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Sample records for chromatography time-scale peptide

  1. Towards Liquid Chromatography Time-Scale Peptide Sequencing and Characterization of Post-Translational Modifications in the Negative-Ion Mode Using Electron Detachment Dissociation Tandem Mass Spectrometry

    DEFF Research Database (Denmark)

    Kjeldsen, Frank; Hørning, Ole B; Jensen, Søren S;

    2008-01-01

    Electron detachment dissociation (EDD) of peptide poly-anions is gentle towards post-translational modifications (PTMs) and produces predictable and interpretable fragment ion types (a., x ions). However, EDD is considered an inefficient fragmentation technique and has not yet been implemented...... in large-scale peptide characterization strategies. We successfully increased the EDD fragmentation efficiency (up to 9%), and demonstrate for the first time the utility of EDD-MS/MS in liquid chromatography time-scale experiments. Peptides and phosphopeptides were analyzed in both positive- and negative...

  2. Optimization of reversed-phase chromatography methods for peptide analytics.

    Science.gov (United States)

    Khalaf, Rushd; Baur, Daniel; Pfister, David

    2015-12-18

    The analytical description and quantification of peptide solutions is an essential part in the quality control of peptide production processes and in peptide mapping techniques. Traditionally, an important tool is analytical reversed phase liquid chromatography. In this work, we develop a model-based tool to find optimal analytical conditions in a clear, efficient and robust manner. The model, based on the Van't Hoff equation, the linear solvent strength correlation, and an analytical solution of the mass balance on a chromatographic column describing peptide retention in gradient conditions is used to optimize the analytical scale separation between components in a peptide mixture. The proposed tool is then applied in the design of analytical reversed phase liquid chromatography methods of five different peptide mixtures. PMID:26620597

  3. Affinity Purification of Insulin by Peptide-Ligand Affinity Chromatography

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The affinity heptapeptide (HWWWPAS) for insulin, selected from phage display library,was coupled to EAH Sepharose 4B gel and packed to a 1-mL column. The column was used for the affinity purification of insulin from protein mixture and commercial insulin preparation. It was observed that the minor impurity in the commercial insulin was removed by the affinity chromatography. Nearly 40 mg of insulin could be purified with the 1-mL affinity column. The results revealed the high specificity and capacity of the affinity column for insulin purification. Moreover, based on the analysis of the amino acids in the peptide sequence, shorter peptides were designed and synthesized for insulin chromatography. As a result, HWWPS was found to be a good alternative to HWWWPAS, while the other two peptides with three or four amino acids showed weak affinity for insulin. The results indicated that the peptide sequence of HWWWPAS was quite conservative for specific binding of insulin.

  4. Diagonal reverse-phase chromatography applications in peptide-centric proteomics: ahead of catalogue-omics?

    Science.gov (United States)

    Gevaert, Kris; Van Damme, Petra; Martens, Lennart; Vandekerckhove, Joël

    2005-10-01

    Diagonal electrophoresis/chromatography was described 40 years ago and was used to isolate specific sets of peptides from simple peptide mixtures such as protease digests of purified proteins. Recently, we have adapted the core technology of diagonal chromatography so that the technique can be used in so-called gel-free, peptide-centric proteome studies. Here we review the different procedures we have developed over the past few years, sorting of methionyl, cysteinyl, amino terminal, and phosphorylated peptides. We illustrate the power of the technique, termed COFRADIC (combined fractional diagonal chromatography), in the case of a peptide-centric analysis of a sputum sol phase sample of a patient suffering from chronic obstructive pulmonary disease (COPD). We were able to identify an unexpectedly high number of intracellular proteins next to known biomarkers.

  5. Stereo-separations of Peptides by Capillary Electrophoresis and Chromatography

    OpenAIRE

    sprotocols

    2014-01-01

    Authors: Afzal Hussain, Iqbal Hussain, Mohamed F. Al-Ajmi & Imran Ali ### Abstract Small peptides (di-, tri-, tetra- penta- hexa etc. and peptides) control many chemical and biological processes. The biological importance of stereomers of peptides is of great value. The stereo-separations of peptides are gaining importance in biological and medicinal sciences and pharmaceutical industries. There is a great need of experimental protocols of stereo-separations of peptides. The vario...

  6. Study on peptide-peptide interaction using high-performance affinity chromatography and quartz crystal microbalance biosensor

    Institute of Scientific and Technical Information of China (English)

    LUO Jia; HUANG YanYan; XIONG ShaoXiang; LIU GuoQuan; ZHAO Rui

    2007-01-01

    The specific interaction between sense and antisense peptides was studied by high-performance affinity chromatography (HPAC) and quartz crystal microbalance (QCM) biosensor. Fragment 1-14 of human interferon-β (hlFN-β) was chosen as sense peptide and its three antisense peptides (AS-IFN 1,AS-IFN 2, and AS-IFN 3) were designed according to the degeneracy of genetic codes. The affinity column was prepared with sense peptide as ligand and the affinity chromatographic behavior was evaluated. Glu-substituted antisense peptide (AS-IFN 3) showed the strongest binding to immobilized sense peptide at pH 7.5. A quartz crystal microbalance-flow injection analysis (QCM-FIA) system was introduced to investigate the recognition process in real-time. The equilibrium dissociation constants between sense peptide and AS-IFN 1, AS-IFN 2 and AS-IFN 3 measured 2.08×10-4, 1.31×10-4 and 2.22×10-5 mol/L, respectively. The mechanism study indicated that the specific recognition between sense peptide and AS-IFN 3 was due to sequence-dependent and multi-modal affinity interaction.

  7. Ensemble Pulsar Time Scale

    Science.gov (United States)

    Yin, D. S.; Gao, Y. P.; Zhao, S. H.

    2016-05-01

    Millisecond pulsars can generate another type of time scale that is totally independent of the atomic time scale, because the physical mechanisms of the pulsar time scale and the atomic time scale are quite different from each other. Usually the pulsar timing observational data are not evenly sampled, and the internals between data points range from several hours to more than half a month. What's more, these data sets are sparse. And all these make it difficult to generate an ensemble pulsar time scale. Hence, a new algorithm to calculate the ensemble pulsar time scale is proposed. Firstly, we use cubic spline interpolation to densify the data set, and make the intervals between data points even. Then, we employ the Vondrak filter to smooth the data set, and get rid of high-frequency noise, finally adopt the weighted average method to generate the ensemble pulsar time scale. The pulsar timing residuals represent clock difference between the pulsar time and atomic time, and the high precision pulsar timing data mean the clock difference measurement between the pulsar time and atomic time with a high signal to noise ratio, which is fundamental to generate pulsar time. We use the latest released NANOGRAV (North American Nanohertz Observatory for Gravitational Waves) 9-year data set to generate the ensemble pulsar time scale. This data set is from the newest NANOGRAV data release, which includes 9-year observational data of 37 millisecond pulsars using the 100-meter Green Bank telescope and 305-meter Arecibo telescope. We find that the algorithm used in this paper can lower the influence caused by noises in timing residuals, and improve long-term stability of pulsar time. Results show that the long-term (> 1 yr) frequency stability of the pulsar time is better than 3.4×10-15.

  8. Selecting protein N-terminal peptides by combined fractional diagonal chromatography.

    Science.gov (United States)

    Staes, An; Impens, Francis; Van Damme, Petra; Ruttens, Bart; Goethals, Marc; Demol, Hans; Timmerman, Evy; Vandekerckhove, Joël; Gevaert, Kris

    2011-07-14

    In recent years, procedures for selecting the N-terminal peptides of proteins with analysis by mass spectrometry have been established to characterize protease-mediated cleavage and protein α-N-acetylation on a proteomic level. As a pioneering technology, N-terminal combined fractional diagonal chromatography (COFRADIC) has been used in numerous studies in which these protein modifications were investigated. Derivatization of primary amines--which can include stable isotope labeling--occurs before trypsin digestion so that cleavage occurs after arginine residues. Strong cation exchange (SCX) chromatography results in the removal of most of the internal peptides. Diagonal, reversed-phase peptide chromatography, in which the two runs are separated by reaction with 2,4,6-trinitrobenzenesulfonic acid, results in the removal of the C-terminal peptides and remaining internal peptides and the fractionation of the sample. We describe here the fully matured N-terminal COFRADIC protocol as it is currently routinely used, including the most substantial improvements (including treatment with glutamine cyclotransferase and pyroglutamyl aminopeptidase to remove pyroglutamate before SCX, and a sample pooling scheme to reduce the overall number of liquid chromatography-tandem mass spectrometry analyses) that were made since its original publication. Completion of the N-terminal COFRADIC procedure takes ~5 d.

  9. Ensemble Pulsar Time Scale

    CERN Document Server

    Rodin, Alexander E

    2010-01-01

    The algorithm of the ensemble pulsar time scale (PT$_{\\rm ens}$) based on the optimal Wiener filtration method has been proposed. This algorithm allows the separation of the contributions to the post-fit pulsar timing residuals of the atomic clock and pulsar itself. Filters were designed with the use of the cross-spectra of the timing residuals. The method has been applied to the timing data of six millisecond pulsars. Direct comparison with the classical method of the weighted average showed that use of the optimal Wiener filters before averaging allows noticeably to improve the fractional instability of the ensemble time scale. Application of the proposed method to the most stable millisecond pulsars with the fractional instability $\\sigma_z < 10^{-15}$ may improve the fractional instability of PT$_{\\rm ens}$ up to the level $\\sim 10^{-16}$.

  10. Improved recovery of proteome-informative, protein N-terminal peptides by combined fractional diagonal chromatography (COFRADIC).

    Science.gov (United States)

    Staes, An; Van Damme, Petra; Helsens, Kenny; Demol, Hans; Vandekerckhove, Joël; Gevaert, Kris

    2008-04-01

    We previously described a proteome-wide, peptide-centric procedure for sorting protein N-terminal peptides and used these peptides as readouts for protease degradome and xenoproteome studies. This procedure is part of a repertoire of gel-free techniques known as COmbined FRActional DIagonal Chromatography (COFRADIC) and highly enriches for alpha-amino-blocked peptides, including alpha-amino-acetylated protein N-terminal peptides. Here, we introduce two additional steps that significantly increase the fraction of such proteome-informative, N-terminal peptides: strong cation exchange (SCX) segregation of alpha-amino-blocked and alpha-amino-free peptides and an enzymatic step liberating pyroglutamyl peptides for 2,4,6-trinitrobenzenesulphonic acid (TNBS) modification and thus COFRADIC sorting. The SCX step reduces the complexity of the analyte mixture by enriching N-terminal peptides and depleting alpha-amino-free internal peptides as well as proline-starting peptides prior to COFRADIC. The action of pyroglutamyl aminopeptidases prior to the first COFRADIC peptide separation results in greatly diminishing numbers of contaminating pyroglutamyl peptides in peptide maps. We further show that now close to 95% of all COFRADIC-sorted peptides are alpha-amino-acetylated and, using the same amount of starting material, our novel procedure leads to an increased number of protein identifications.

  11. Determination of theoretical retention times for peptides analyzed by reversed- -phase high-performance liquid chromatography

    Directory of Open Access Journals (Sweden)

    Jerzy Dziuba

    2011-06-01

    Full Text Available   Background. Peptides are important components of foods mainly due to their biological activity. The basic method of their identification is reversed phase high-performance liquid chromatography coupled with electrospray-ionization mass spectrometry (RP-HPLC-ESI-MS. Retention time (tR prediction in silico is very helpful in analysis of multicomponent peptide mixtures. One of problems associated with RP-HPLC-ESI-MS is deterioration of mass spectra quality by trifluoroacetic acid (TFA. This problem can be avoided through the use of chromatographic columns designed for work with low TFA concentrations in mobile phase. The objective of this study was to determine the correlations between peptide retention times predicted with the use of a program available on-line and experimental retention times obtained using the column working with low TFA concentrations. Material and methods. The set of synthetic peptides and bovine α-lactalbumin fragments (18 peptides was used in the experiment. Theoretical retention times were calculated using Sequence Specific Retention Calculator (SSRC program. The experimental retention times were measured via RP-HPLC-ESI-MS method using column working with low TFA content. The dependence between theoretical and experimental tR was expressed via empirical equations. Results. The best correlation between theoretical and experimental retention times of peptides containing at least four amino acid residues has been obtained when third order polynomial (R² = 0.9536. Prediction quality for di- and tripeptides was significantly lower. The method described can be applied for cysteine-containing peptides although our sample preparation procedure did not include modification of this amino acid, taken into attention by SSRC program. Conclusions. The results of this study validate the usefulness of a third degree polynomial as a simple function describing the correlation between peptide retention times predicted by an on

  12. On the Geologic Time Scale

    NARCIS (Netherlands)

    Gradstein, F.M.; Ogg, J.G.; Hilgen, F.J.

    2012-01-01

    This report summarizes the international divisions and ages in the Geologic Time Scale, published in 2012 (GTS2012). Since 2004, when GTS2004 was detailed, major developments have taken place that directly bear and have considerable impact on the intricate science of geologic time scaling. Precam br

  13. Chromatography.

    Science.gov (United States)

    Brantley, L. Reed, Sr.; Demanche, Edna L.; Klemm, E. Barbara; Kyselka, Will; Phillips, Edwin A.; Pottenger, Francis M.; Yamamoto, Karen N.; Young, Donald B.

    This booklet presents some activities on chromatography. Directions for preparing leaf pigment extracts using alcohol are given, and paper chromatography and thin-layer chromatography are described as modifications of the basic principles of chromatography. (KHR)

  14. Identification of Nitrotyrosine Containing Peptides using Combined Fractional Diagonal Chromatography (COFRADIC) and Off-Line Nano-LC-MALDI

    DEFF Research Database (Denmark)

    Larsen, Trine R; Bache, Nicolai; Gramsbergen, Jan Bert;

    2011-01-01

    , the study of these proteins has been compromised by the lack of good methods for identifying nitrated proteins, their nitration sites and the level of nitration. Here, we present a method for identification of nitrated peptides that allows the site specific assignment of nitration, is easy to use...... and reproducible, and opens up for the possibility to quantify the level of nitration of specific peptides as function of different oxidative conditions, namely combined fractional diagonal chromatography (COFRADIC) in combination with off-line nano-LC-MALDI. We identify six nitrated peptides from in vitro...

  15. Enrichment and Analysis of Nonenzymatically Glycated Peptides: Boronate Affinity Chromatography Coupled with Electron-Transfer Dissociation Mass Spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Qibin; Tang, Ning; Brock, Jonathan W.; Mottaz, Heather M.; Ames, Jennifer M.; Baynes, John; Smith, Richard D.; Metz, Thomas O.

    2007-06-01

    Non-enzymatic glycation of peptides and proteins by D-glucose has important implications in the pathogenesis of diabetes mellitus, particularly in the development of diabetic complications. However, no effective high-throughput methods exist for identifying proteins containing this low abundance post-translational modification in bottom-up proteomic studies. In this report, phenylboronate affinity chromatography was used in a two-step enrichment scheme to selectively isolate first glycated proteins and then glycated, tryptic peptides from human serum glycated in vitro. Enriched peptides were subsequently analyzed by alternating electron transfer dissociation (ETD) and collision induced dissociation (CID) tandem mass spectrometry. It was observed that ETD fragmentation mode resulted in a significantly higher number of glycated peptide identifications (87.6% of all identified peptides) versus CID mode (17.0% of all identified peptides), when utilizing dual glycation enrichment on both the protein and peptide level. This study illustrates that phenylboronate affinity chromatography coupled with LC-MS/MS with ETD as the fragmentation mode is an efficient approach for analyses of glycated proteins and can have broad applications in studies of diabetes mellitus.

  16. Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis

    OpenAIRE

    Young Chon Choi; Ki Rok, Kwon; Suk Ho, Choi

    2006-01-01

    Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. Results : Melittin and other peptide components were separated from bee venom by using gel filtration...

  17. Relative Quantification of Sites of Peptide and Protein Modification Using Size Exclusion Chromatography Coupled with Electron Transfer Dissociation

    Science.gov (United States)

    Xie, Boer; Sharp, Joshua S.

    2016-08-01

    One difficult problem in the analysis of peptide modifications is quantifying isomeric modifications that differ by the position of the amino acid modified. HPLC separation using C18 reverse phase chromatography coupled with electron transfer dissociation (ETD) in tandem mass spectrometry has recently been shown to be able to relatively quantify how much of a given modification occurs at each amino acid position for isomeric mixtures; however, the resolution of reverse phase chromatography greatly complicates quantification of isomeric modifications by ETD because of the chromatographic separation of peptides with identical modifications at different sequence positions. Using peptide oxidation as a model system, we investigated the use of size exclusion chromatography coupled with ETD fragmentation to separate peptide sequences. This approach allows for the benefits of chromatographic separation of peptide sequences while ensuring co-elution of modification isomers for accurate relative quantification of modifications using standard data-dependent acquisitions. Using this method, the relative amount of modification at each amino acid can be accurately measured from single ETD MS/MS spectra in a standard data-dependent acquisition experiment.

  18. Relative Quantification of Sites of Peptide and Protein Modification Using Size Exclusion Chromatography Coupled with Electron Transfer Dissociation.

    Science.gov (United States)

    Xie, Boer; Sharp, Joshua S

    2016-08-01

    One difficult problem in the analysis of peptide modifications is quantifying isomeric modifications that differ by the position of the amino acid modified. HPLC separation using C18 reverse phase chromatography coupled with electron transfer dissociation (ETD) in tandem mass spectrometry has recently been shown to be able to relatively quantify how much of a given modification occurs at each amino acid position for isomeric mixtures; however, the resolution of reverse phase chromatography greatly complicates quantification of isomeric modifications by ETD because of the chromatographic separation of peptides with identical modifications at different sequence positions. Using peptide oxidation as a model system, we investigated the use of size exclusion chromatography coupled with ETD fragmentation to separate peptide sequences. This approach allows for the benefits of chromatographic separation of peptide sequences while ensuring co-elution of modification isomers for accurate relative quantification of modifications using standard data-dependent acquisitions. Using this method, the relative amount of modification at each amino acid can be accurately measured from single ETD MS/MS spectra in a standard data-dependent acquisition experiment. Graphical Abstract ᅟ. PMID:27075875

  19. Statistical inference across time scales

    CERN Document Server

    Duval, Céline

    2011-01-01

    We investigate statistical inference across time scales. We take as toy model the estimation of the intensity of a discretely observed compound Poisson process with symmetric Bernoulli jumps. We have data at different time scales: microscopic, intermediate and macroscopic. We quantify the smooth statistical transition from a microscopic Poissonian regime to a macroscopic Gaussian regime. The classical quadratic variation estimator is efficient in both microscopic and macroscopic scales but surprisingly shows a substantial loss of information in the intermediate scale that can be explicitly related to the sampling rate. We discuss the implications of these findings beyond this idealised framework.

  20. Integral equations on time scales

    CERN Document Server

    Georgiev, Svetlin G

    2016-01-01

    This book offers the reader an overview of recent developments of integral equations on time scales. It also contains elegant analytical and numerical methods. This book is primarily intended for senior undergraduate students and beginning graduate students of engineering and science courses. The students in mathematical and physical sciences will find many sections of direct relevance. The book contains nine chapters and each chapter is pedagogically organized. This book is specially designed for those who wish to understand integral equations on time scales without having extensive mathematical background.

  1. Symmetric Differentiation on Time Scales

    OpenAIRE

    da Cruz, Artur M. C. Brito; Martins, Natalia; Delfim F. M. Torres

    2012-01-01

    We define a symmetric derivative on an arbitrary nonempty closed subset of the real numbers and derive some of its properties. It is shown that real-valued functions defined on time scales that are neither delta nor nabla differentiable can be symmetric differentiable.

  2. Peptides Quantification by Liquid Chromatography with Matrix-Assisted Laser Desorption/Ionization and Selected Reaction Monitoring Detection

    OpenAIRE

    Lesur, Antoine; Varesio, Emmanuel; Domon, Bruno; Hopfgartner, Gérard

    2012-01-01

    We present a novel analytical platform for peptides quantitative assays in biological matrices based on microscale liquid chromatography fractionation and matrix-assisted laser desorption/ionization mass spectrometric detection using the selected reaction monitoring (SRM) mode. The MALDI source was equipped with a high frequency Nd:YAG laser (1000 Hz) and mounted on a triple quadrupole / linear ion trap mass spectrometer (MALDI-QqQLIT). Compared to conventional LC-ESI-SRM/MS, the separated an...

  3. Time Scales in Spectator Fragmentation

    CERN Document Server

    Schwarz, C; Bassini, R; Begemann-Blaich, M L; Gaff-Ejakov, S J; Gourio, D; Gross, C; Imme, G; Iori, I; Kleinevoss, U; Kunde, G J; Kunze, W D; Lynen, U; Maddalena, V; Mahi, M; Möhlenkamp, T; Moroni, A; Müller, W F J; Nociforo, C; Ocker, B; Odeh, T; Petruzzelli, F; Pochodzalla, J; Raciti, G; Riccobene, G; Romano, F; Saija, A; Schnittker, M; Schüttauf, A; Seidel, W; Serfling, V; Sfienti, C; Trautmann, W; Trzcinski, A; Verde, G; Wörner, A; Hong Fei Xi; Zwieglinski, B

    2001-01-01

    Proton-proton correlations and correlations of p-alpha, d-alpha, and t-alpha from spectator decays following Au + Au collisions at 1000 AMeV have been measured with an highly efficient detector hodoscope. The constructed correlation functions indicate a moderate expansion and low breakup densities similar to assumptions made in statistical multifragmentation models. In agreement with a volume breakup rather short time scales were deduced employing directional cuts in proton-proton correlations. PACS numbers: 25.70.Pq, 21.65.+f, 25.70.Mn

  4. Inversion of type of separation system in planar chromatography of peptides, using C18 silica-based adsorbents.

    Science.gov (United States)

    Gwarda, Radosław Ł; Aletańska-Kozak, Monika; Matosiuk, Dariusz; Dzido, Tadeusz H

    2016-04-01

    Our previous results show, that C18 silica-based adsorbents used in high-performance thin-layer chromatography (HPTLC), provide complex retention mechanism basing on various polar and nonpolar interactions. Here we present, that in chromatography of peptides, due to mixed-mode properties of these adsorbents, there is a simple way to obtain inversion of separation system type (from reversed-phase, RP, to normal-phase, NP, and vice versa). The results presented provide detailed information how to obtain inversion mentioned and reflect the extent (the type and concentration of organic solvent, the type and concentration of ion-pairing reagent in the mobile phase) of this phenomenon. We show, that the system type inversion results in significant change of selectivity of separation, which may be especially useful in 2D separation of complex samples of basic/amphoteric compounds such as peptides. This results from the fact, that C18 silica-based HPTLC adsorbents, may be used in hydrophilic interaction chromatography (HILIC) or RP chromatography, in dependence on mobile phase composition.

  5. Purification and identification of O-GlcNAc-modified peptides using phosphate-based alkyne CLICK chemistry in combination with titanium dioxide chromatography and mass spectrometry

    DEFF Research Database (Denmark)

    Parker, Benjamin L; Gupta, Pankaj; Cordwell, Stuart;

    2011-01-01

    -containing peptides were enriched using titanium dioxide chromatography. Modified peptides were analyzed using a combination of higher energy collision dissociation for identification and electron transfer dissociation to localize the site of O-GlcNAc attachment. The enrichment method was developed...

  6. Method for enhanced accuracy in predicting peptides using liquid separations or chromatography

    Science.gov (United States)

    Kangas, Lars J.; Auberry, Kenneth J.; Anderson, Gordon A.; Smith, Richard D.

    2006-11-14

    A method for predicting the elution time of a peptide in chromatographic and electrophoretic separations by first providing a data set of known elution times of known peptides, then creating a plurality of vectors, each vector having a plurality of dimensions, and each dimension representing the elution time of amino acids present in each of these known peptides from the data set. The elution time of any protein is then be predicted by first creating a vector by assigning dimensional values for the elution time of amino acids of at least one hypothetical peptide and then calculating a predicted elution time for the vector by performing a multivariate regression of the dimensional values of the hypothetical peptide using the dimensional values of the known peptides. Preferably, the multivariate regression is accomplished by the use of an artificial neural network and the elution times are first normalized using a transfer function.

  7. Separation of peptides and intact proteins by electrostatic repulsion reversed phase liquid chromatography.

    Science.gov (United States)

    Gritti, Fabrice; Guiochon, Georges

    2014-12-29

    A new brand of BEH-C18 hybrid particles chemically bonded to a leash carrying an amine group permits the implementation of electrostatic repulsive interactions chromatography. Using columns packed with this material, the influence of the concentration of positive charges bonded to the BEH-C18 surface on the overloaded band profiles of a few positively charged peptides and proteins was investigated in the gradient elution mode. Three columns packed with endcapped BEH-C18 particles bonded with three different surface-charge densities (LOW, MEDIUM and HIGH) were used and compared with those provided by a column packed with non-doped, endcapped BEH-C18 particles. The surface concentrations of fixed charges in the LOW, MEDIUM and HIGH columns were estimated at 0.029, 0.050, and 0.064μmol/m(2), for example, about two orders of magnitude smaller than the surface density of bonded C18 chains (2.1μmol/m(2)). Three different mobile phase additives (0.1% v/v of trifluoro-acetic, phosphoric, and formic acid) were used to optimize the purification levels of proteins under different loading conditions. The weak ion-pairing ions (formate and phosphate) generate smaller retention but broader, more fronting band profiles than those eluted with a stronger ion-pairing ion (trifluoroactate). This effect is worse in the presence of fixed charges at the surface of the BEH-C18 particles. This was explained by an enhanced anti-Langmuirian adsorption behavior of the charged proteins in the presence of fixed surface charges. As the protein concentration increases in the bulk, so does the internal ionic strength, the electrostatic repulsive interactions weaken, and retention increases. Band fronting is mostly eliminated by replacing weak ion-pairing acids with TFA with which the adsorption isotherm remains weakly langmuirian. Faster but still complete gradient separation of insulin and myoglobin were achieved with the HIGH column than with the reference neutral column, despite a measurable

  8. Purification of Peptide Components including Melittin from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis

    Directory of Open Access Journals (Sweden)

    Young Chon Choi

    2006-06-01

    Full Text Available Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. Results : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. The fractions obtained from gel filtration chromatography was analyzed by using SDS-PAGE and propionic acid/urea polyacrylamide gel electrophoresis. The melittin obtained from the gel filtration contained residual amount of phospholipase A2 and a protein with molecular weight of 6,000. The contaminating proteins were removed by the second gel filtration chromatography. Conclusion : Gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis are useful to separate peptide components including melittin from bee venom.

  9. Purification and identification of endogenous antioxidant and ACE-inhibitory peptides from donkey milk by multidimensional liquid chromatography and nanoHPLC-high resolution mass spectrometry.

    Science.gov (United States)

    Zenezini Chiozzi, Riccardo; Capriotti, Anna Laura; Cavaliere, Chiara; La Barbera, Giorgia; Piovesana, Susy; Samperi, Roberto; Laganà, Aldo

    2016-08-01

    Donkey milk is a valuable product for the food industry due to its nutraceutical, nutritional, and functional properties. In this work, the endogenous peptides from donkey milk were investigated for their antioxidant and ACE-inhibitory activities, combining a two-dimensional peptide fractionation strategy with high-resolution mass spectrometry, bioinformatics analysis, and in vitro assays. After extraction, the endogenous peptides were fractionated twice, first by polymeric reversed phase and then by hydrophilic interaction chromatography. Fractions were screened for the investigated bioactivities and only the most active ones were finally analyzed by nanoRP-HPLC-MS/MS; this approach allowed to reduce the total number of possible bioactive sequences. Results were further mined by in silico analysis using PeptideRanker, BioPep, and PepBank, which provided a bioactivity score to the identified peptides and matched sequences to known bioactive peptides, in order to select candidates for chemical synthesis. Thus, five peptides were prepared and then compared to the natural occurring ones, checking their retention times and fragmentation patterns in donkey milk alone and in spiked donkey milk samples. Pure peptide standards were finally in vitro tested for the specific bioactivity. In this way, two novel endogenous antioxidant peptides, namely EWFTFLKEAGQGAKDMWR and GQGAKDMWR, and two ACE-inhibitory peptides, namely REWFTFLK and MPFLKSPIVPF, were successfully validated from donkey milk. Graphical Abstract Analytical workflow for purification and identification of bioactive peptides from donkey milk sample. PMID:27325462

  10. Advances in chiral separations of small peptides by capillary electrophoresis and chromatography.

    Science.gov (United States)

    Ali, Imran; Al-Othman, Zeid A; Al-Warthan, Abdulrahman; Asnin, Leonid; Chudinov, Alexander

    2014-09-01

    Many chemical and biological processes are controlled by the stereochemistry of small polypeptides (di-, tri-, tetra-, penta-, hexapeptides, etc). The biological importance of peptide stereoisomers is of great value. Therefore, the chiral resolution of peptides is an important issue in biological and medicinal sciences and drug industries. The chiral resolutions of peptide racemates have been discussed with the use of capillary electrophoresis and chromatographic techniques. The various chiral selectors used were polysaccharides, cyclodextrins, Pirkle types, macrocyclic antibiotics, crown ethers, imprinted polymers, etc. The stereochemistry of dipeptides is also discussed. Besides, efforts are made to explain the chiral recognition mechanisms, which will be helpful in understanding existing and developing new stereoselective analyses. Future perspectives of enantiomeric resolution are also predicted. Finally, the review concludes with the demand of enantiomeric resolution of all naturally occurring and synthetic peptides. PMID:25044566

  11. Stochastic dynamic equations on general time scales

    OpenAIRE

    Martin Bohner; Olexandr M. Stanzhytskyi; Anastasiia O. Bratochkina

    2013-01-01

    In this article, we construct stochastic integral and stochastic differential equations on general time scales. We call these equations stochastic dynamic equations. We provide the existence and uniqueness theorem for solutions of stochastic dynamic equations. The crucial tool of our construction is a result about a connection between the time scales Lebesgue integral and the Lebesgue integral in the common sense.

  12. Kalman plus weights: a time scale algorithm

    Science.gov (United States)

    Greenhall, C. A.

    2001-01-01

    KPW is a time scale algorithm that combines Kalman filtering with the basic time scale equation (BTSE). A single Kalman filter that estimates all clocks simultaneously is used to generate the BTSE frequency estimates, while the BTSE weights are inversely proportional to the white FM variances of the clocks. Results from simulated clock ensembles are compared to previous simulation results from other algorithms.

  13. Some integral inequalities on time scales

    Institute of Scientific and Technical Information of China (English)

    Adnan Tuna; Servet Kutukcu

    2008-01-01

    In this article, we study the reverse Holder type inequality and Holder in-equality in two dimensional case on time scales. We also obtain many integral inequalities by using H(o)lder inequalities on time scales which give Hardy's inequalities as spacial cases.

  14. Peptides quantification by liquid chromatography with matrix-assisted laser desorption/ionization and selected reaction monitoring detection.

    Science.gov (United States)

    Lesur, Antoine; Varesio, Emmanuel; Domon, Bruno; Hopfgartner, Gérard

    2012-10-01

    We present a novel analytical platform for peptides quantitative assays in biological matrices based on microscale liquid chromatography fractionation and matrix-assisted laser desorption/ionization mass spectrometric detection using the selected reaction monitoring (SRM) mode. The MALDI source was equipped with a high frequency Nd:YAG laser (1000 Hz) and mounted on a triple quadrupole/linear ion trap mass spectrometer (MALDI-QqQ(LIT)). Compared to conventional LC-ESI-SRM/MS, the separated analytes are "time-frozen" onto the MALDI plate in fractions, and navigation through the LC chromatogram makes it possible to perform SRM experiments as well as enhanced product ion spectra acquisition for confirmatory analyses without time constraints. The LC spots were analyzed using different rastering speeds ranging from 0.25 to 4 mm/sec with the shortest analysis time of 425 ms/spot. Since the LC runs can be multiplexed and do not need a comprehensive investigation, the present platform offers a valuable alternative to LC-ESI-SRM/MS for high throughput proteomic analyses. In addition, the derivatization of the N-terminal α-amino group by sulfonation was found to be key for the fragmentation of singly charged peptides under low collision energy regime. Under such conditions, y-ion series were observed in the MS/MS spectra, and thus the design of SRM experiments was greatly simplified. The quantitative performance of the platform was compared to that of LC-ESI-SRM/MS by spiking yeast tryptic peptides in human plasma digests. Both platforms exhibited similar sensitivities, accuracy (within ±20%) and precision (under 20%) in the relative quantification mode. As a proof of principle, the relative and absolute quantification of proteins associated with glycolysis, glyoxylate and tricarboxylic acid (TCA) cycles over a growth time course of Saccharomyces cerevisiae on glucose media was successfully performed using isotopic dilution. PMID:22897511

  15. Highly efficient and selective enrichment of peptide subsets combining fluorous chemistry with reversed-phase chromatography

    OpenAIRE

    Ying, Wantao; Perlman, David H.; Li, Lei(Beijing Institute of Petrochemical Technology, Beijing, 102617, People's Republic of China); Théberge, Roger; Costello, Catherine E; McComb, Mark E.

    2009-01-01

    The selective capture of target peptides poses a great challenge to modern chemists and biologists, especially when enriching them from proteome samples possessing extremes in concentration dynamic range and sequence diversity. While approaches based on traditional techniques such as biotin-avidin pairing offer versatile tools to design strategies for selective enrichment, problems are still encountered due to sample loss or poor selectivity of enrichment. Here we show that the recently intro...

  16. Time Scale in Least Square Method

    Directory of Open Access Journals (Sweden)

    Özgür Yeniay

    2014-01-01

    Full Text Available Study of dynamic equations in time scale is a new area in mathematics. Time scale tries to build a bridge between real numbers and integers. Two derivatives in time scale have been introduced and called as delta and nabla derivative. Delta derivative concept is defined as forward direction, and nabla derivative concept is defined as backward direction. Within the scope of this study, we consider the method of obtaining parameters of regression equation of integer values through time scale. Therefore, we implemented least squares method according to derivative definition of time scale and obtained coefficients related to the model. Here, there exist two coefficients originating from forward and backward jump operators relevant to the same model, which are different from each other. Occurrence of such a situation is equal to total number of values of vertical deviation between regression equations and observation values of forward and backward jump operators divided by two. We also estimated coefficients for the model using ordinary least squares method. As a result, we made an introduction to least squares method on time scale. We think that time scale theory would be a new vision in least square especially when assumptions of linear regression are violated.

  17. Hardy type inequalities on time scales

    CERN Document Server

    Agarwal, Ravi P; Saker, Samir H

    2016-01-01

    The book is devoted to dynamic inequalities of Hardy type and extensions and generalizations via convexity on a time scale T. In particular, the book contains the time scale versions of classical Hardy type inequalities, Hardy and Littlewood type inequalities, Hardy-Knopp type inequalities via convexity, Copson type inequalities, Copson-Beesack type inequalities, Liendeler type inequalities, Levinson type inequalities and Pachpatte type inequalities, Bennett type inequalities, Chan type inequalities, and Hardy type inequalities with two different weight functions. These dynamic inequalities contain the classical continuous and discrete inequalities as special cases when T = R and T = N and can be extended to different types of inequalities on different time scales such as T = hN, h > 0, T = qN for q > 1, etc.In this book the authors followed the history and development of these inequalities. Each section in self-contained and one can see the relationship between the time scale versions of the inequalities and...

  18. Peptide mapping of /sup 125/I-labelled membrane protein of influenza viruses by reverse-phase high-performance liquid chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Darveau, A.; Lecomte, J. (Centre de Recherche en Virologie, Institut Armand-Frappier, Laval, Quebec (Canada)); Seidah, N.G.; Chretien, M. (Institut de Recherches Cliniques de Montreal, Montreal (Canada))

    1982-03-01

    The resolution potential of reverse-phase high-performance liquid chromatography (HPLC) for peptide analysis of hydrophobic viral membranes has been investigated, using as a model the membrane (M) protein of influenza virus. Proteolytic digests of /sup 125/I-labelled M protein CNBr fragments, extracted from radioiodinated whole virus, have been separated on a uBondapak C/sub 18/ column with an isopropanol or acetonitrile solvent system. Peptide mapping of trypsin digests of M protein from A/PR/8/34 (H1N1) and A/chicken/Germany/N/49 (H10N7) viruses was identical, whereas Staphylococcus aureus V8 protease digests showed minor differences in at least two peptides. The results also show that HPLC is a powerful tool for the separation of proteolytic digests of viral proteins, since the peptide maps are highly reproducible and recovery was always greater than 85%.

  19. Identification and quantification of peptides and proteins secreted from prostate epithelial cells by unbiased liquid chromatography tandem mass spectrometry using goodness of fit and analysis of variance.

    Science.gov (United States)

    Florentinus, Angelica K; Bowden, Peter; Sardana, Girish; Diamandis, Eleftherios P; Marshall, John G

    2012-02-01

    The proteins secreted by prostate cancer cells (PC3(AR)6) were separated by strong anion exchange chromatography, digested with trypsin and analyzed by unbiased liquid chromatography tandem mass spectrometry with an ion trap. The spectra were matched to peptides within proteins using a goodness of fit algorithm that showed a low false positive rate. The parent ions for MS/MS were randomly and independently sampled from a log-normal population and therefore could be analyzed by ANOVA. Normal distribution analysis confirmed that the parent and fragment ion intensity distributions were sampled over 99.9% of their range that was above the background noise. Arranging the ion intensity data with the identified peptide and protein sequences in structured query language (SQL) permitted the quantification of ion intensity across treatments, proteins and peptides. The intensity of 101,905 fragment ions from 1421 peptide precursors of 583 peptides from 233 proteins separated over 11 sample treatments were computed together in one ANOVA model using the statistical analysis system (SAS) prior to Tukey-Kramer honestly significant difference (HSD) testing. Thus complex mixtures of proteins were identified and quantified with a high degree of confidence using an ion trap without isotopic labels, multivariate analysis or comparing chromatographic retention times.

  20. Further investigation of a peptide extraction method with mesoporous silica using high-performance liquid chromatography coupled with tandem mass spectrometry.

    Science.gov (United States)

    Du, Yan; Wu, Dapeng; Guan, Yafeng

    2016-06-01

    Mobil Composition of Matter No. 41 (MCM-41) was the most frequently used mesoporous silica material to extract peptides from complex biological samples. However, there were confusing extraction conditions and large extraction efficiency variance among related reports, which resulted from unclear understanding about the interaction between the material and peptides. In this study, the extraction mechanism was investigated with one set of tryptic peptides by using high-performance liquid chromatography coupled with triple quadrupole mass spectrometry. Generally, hydrophobic interaction and electrostatic attraction were two major driving forces for extraction of peptides, while electrostatic repulsion greatly weakened the interaction between the material and peptides with isoelectric points below the pH. With most peptides positively charged and MCM-41 slightly negatively charged, most efficient extraction was obtained at pH 3, and it was proved that electrostatic and hydrophobic interaction acted in synergy for extraction of all the peptides. A mixed solution of acetonitrile with buffers of high pH or ion strength was demonstrated to be favorable for elution, which performed much better than the commonly used eluate (mixture of acetonitrile with 0.1% trifluoroacetic acid). Finally, under optimum conditions, it was found that extraction efficiency of MCM-41 for protein digest and human serum was greatly improved. PMID:27059091

  1. The perchlorate anion is more effective than the trifluoroacetate anion as an ion-pairing reagent for reversed-phase chromatography of peptides

    OpenAIRE

    Shibue, M.; Mant, C.T.; Hodges, R.S.

    2005-01-01

    The addition of salts, specifically sodium perchlorate (NaClO4), to mobile phases at acidic pH as ion-pairing reagents for reversed-phase high-performance liquid chromatography (RP-HPLC) has been generally overlooked. To demonstrate the potential of NaClO4 as an effective anionic ion-pairing reagent, we applied RP-HPLC in the presence of 0–100 mM sodium chloride (NaCl), sodium trifluoroacetate (NaTFA) or NaClO4 to two mixtures of synthetic 18-residue peptides: a mixture of peptides with the s...

  2. Far-ultraviolet absorbance detection of sugars and peptides by high-performance liquid chromatography.

    Science.gov (United States)

    Uchiho, Yuichi; Goto, Yusuke; Kamahori, Masao; Aota, Toshimichi; Morisaki, Atsuki; Hosen, Yusuke; Koda, Kimiyoshi

    2015-12-11

    A far-ultraviolet (FUV)-absorbance detector with a transmission flow cell was developed and applied to detect absorbance of sugars and peptides by HPLC. The main inherent limitation of FUV-absorbance detection is the strong absorptions of solvents and atmospheric oxygen in the optical system as well as dissolved oxygen in the solvent. High absorptivity of the solvent and oxygen decreases transmission-light intensity in the flow cell and hinders the absorbance measurement. To solve the above drawbacks, the transmission-light intensity in the flow cell was increased by introducing a new optical system and a nitrogen-purging unit to remove the atmospheric oxygen. The optical system has a photodiode for detecting the reference light at a position of the minus-first-order diffracted light. In addition, acetonitrile and water were selected as usable solvents because of their low absorptivity in the FUV region. As a result of these implementations, the detectable wavelength of the FUV-absorbance detector (with a flow cell having an effective optical path length of 0.5mm) can be extended down to 175nm. Three sugars (glucose, fructose, and sucrose) were successfully detected with the FUV-absorbance detector. These detection results reveal that the absorption peak of sugar in liquid phase lies at around 178nm. The detection limit (S/N=3) in absorbance with a 0.5-mm flow cell at 180nm was 21μAU, which corresponds to 33, 60 and 60μM (198, 360, and 360pmol) for fructose, glucose, and sucrose, respectively. Also, the peptide Met-enkephalin could be detected with a high sensitivity at 190nm. The estimated detection limit (S/N=3) for Met-enkephalin is 29nM (0.29pmol), which is eight times lower than that at 220nm. PMID:26596871

  3. Determination of aromatic and sulfur-containing amino acids, peptides, and proteins using high-performance liquid chromatography with photolytic electrochemical detection

    Energy Technology Data Exchange (ETDEWEB)

    Dou, Lin; Krull, I.S. (Northeastern Univ., Boston, MA (USA))

    1990-12-01

    Aromatic amino acids, sulfur-containing amino acids, peptides containing such constituents, and proteins can now be detected in high-performance liquid chromatography by the use of on-line, postcolumn, continuous photolytic derivatization with electrochemical (HPLC-h{nu}-EC) detection. The overall approach is a very simple, reproducible, rapid, and fully automatable approach for the determination of certain amino acids, peptides, and proteins with excellent selectivity, sensitivity, and linearities of response. Dual-electrode response ratios, lamp-on/lamp-off behavior, and chromatographic capacity factors all contribute to the enhanced selectivity of the overall HPLC-h{nu}-EC determination for these particular classes of bioorganics and biopolymers. The analytical figures of merit, chromatography detection, and method validation approaches have all be optimally derived and demonstrated reproducible. Applications of the basic methodology to real-world samples are demonstrated and validated.

  4. Peptides from two sanguinovorous leeches analyzed by ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometric detector

    Directory of Open Access Journals (Sweden)

    Ling Xiao

    2015-01-01

    Full Text Available Background: Hirudo nipponica Whitman and Poecilobdella manillensis Lesson fall into the family of Hirudinidae Whitman, both of them are sanguinovorous leeches and used a anticoagulant medicines in China. Their medicinal parts are the dried bodies. However, the peptides in the dried body of the two leeches have not been very clear up to now. Objective: To analyze the peptides from two sanguinovorous leeches, H. nipponica and P. manillensis. Materials and Methods: In this article it is reported that the peptides were obtained from anticoagulant active extracted parts of dried bodies of the two leeches and their molecular weights were analyzed by ultra-performance liquid chromatography with electrospray ionization quadrupole time-of-flight mass spectrometry mass spectrometric detector online. Results: Three peptide components were identified from H. nipponica with their molecular weight separately 14998, 15988, and 15956, six peptide components were identified from P. manillensis with molecular weight 9590, 13642, 14998, 17631, 15988, and 16567. Two of peptides from P. manillensis have the same molecular weight 14998 and 15988 as that in H. nipponica. Conclusion: And the two peptides are the main peaks in the base peak ion chromatogram because they occupied a large ratio of total base peak area. Hence the composition of the extracted active part of the two leeches are very close, difference is in that the extract of P. manillensis has more small peptide peaks, but the extract of H. nipponica has not. Furthermore, the tryptic digestion hydrolysates of the extracted active part of each sample were analyzed and the results showed that there were four peaks which only exist in P. manillensis, but not in Hirudo nipponia. They may be the identified peak between the two leeches. This work support the viewpoint that P. manillensis can be used as a medicinal leech as H. nipponia and these peptide components of dried bodies of the two species leeches are a

  5. High performance liquid chromatography (HPLC) fingerprints and primary structure identification of corn peptides by HPLC-diode array detection and HPLC-electrospray ionization tandem mass spectrometry

    OpenAIRE

    Chi Wang; Hui He; Jiu-liang Zhang; Xing Li; Zhi-li Ma

    2016-01-01

    Corn peptides (CPs) are reported to have many biological functions, such as facilitating alcohol metabolism, antioxidation, antitumor, antihypertension, and hepatoprotection. To develop a method for quality control, the high-performance liquid chromatography (HPLC) system was applied. Twenty-eight common peaks were found in all the CPs of corn samples from Enshi, China, based on which, a fingerprinting chromatogram was established for use in quality control in future research. Subsequently, t...

  6. Some Nonlinear Integral Inequalities on Time Scales

    OpenAIRE

    Li Wei Nian; Sheng Weihong

    2007-01-01

    The purpose of this paper is to investigate some nonlinear integral inequalities on time scales. Our results unify and extend some continuous inequalities and their corresponding discrete analogues. The theoretical results are illustrated by a simple example at the end of this paper.

  7. Some Nonlinear Integral Inequalities on Time Scales

    Directory of Open Access Journals (Sweden)

    Li Wei Nian

    2007-01-01

    Full Text Available The purpose of this paper is to investigate some nonlinear integral inequalities on time scales. Our results unify and extend some continuous inequalities and their corresponding discrete analogues. The theoretical results are illustrated by a simple example at the end of this paper.

  8. The Second Noether Theorem on Time Scales

    Directory of Open Access Journals (Sweden)

    Agnieszka B. Malinowska

    2013-01-01

    Full Text Available We extend the second Noether theorem to variational problems on time scales. As corollaries we obtain the classical second Noether theorem, the second Noether theorem for the h-calculus and the second Noether theorem for the q-calculus.

  9. Some Nonlinear Dynamic Inequalities on Time Scales

    Indian Academy of Sciences (India)

    Wei Nian Li; Weihong Sheng

    2007-11-01

    The aim of this paper is to investigate some nonlinear dynamic inequalities on time scales, which provide explicit bounds on unknown functions. The inequalities given here unify and extend some inequalities in (B G Pachpatte, On some new inequalities related to a certain inequality arising in the theory of differential equation, J. Math. Anal. Appl. 251 (2000) 736--751).

  10. Prediction of Molar Extinction Coefficients of Proteins and Peptides Using UV Absorption of the Constituent Amino Acids at 214 nm To Enable Quantitative Reverse Phase High-Performance Liquid Chromatography-Mass Spectrometry Analysis

    NARCIS (Netherlands)

    Kuipers, B.J.H.; Gruppen, H.

    2007-01-01

    The molar extinction coefficients of 20 amino acids and the peptide bond were measured at 214 nm in the presence of acetonitrile and formic acid to enable quantitative comparison of peptides eluting from reversed-phase high-performance liquid chromatography, once identified with mass spectrometry (R

  11. Turbulence time scales in mixing box experiments

    Energy Technology Data Exchange (ETDEWEB)

    Dohan, K.; Sutherland, B.R. [Department of Mathematical and Statistical Sciences, University of Alberta, Edmonton, AB, T6G 2G1 (Canada)

    2002-11-01

    Laboratory experiments are performed to examine eddy time scales in turbulence generated by an oscillating grid in homogeneous fluid using tanks with varying depth and fixed high aspect ratio horizontal cross-section. For high ratios of depth to width, a mean circulation develops in the form of a pair of counter-rotating vortices. In a new technique, pearlescent dye is employed to measure eddy time scales and to determine their power law scaling dependence on the distance from the oscillating grid. Two scaling regimes are observed, one near the source of turbulence and one at intermediate depths. At intermediate depths, the scaling exponent is found to increase if the total fluid depth is larger. We discuss the significance of these results on the use of the law-of-the-wall scaling in the upper oceanic mixed layer. (orig.)

  12. Liquidity crises on different time scales

    Science.gov (United States)

    Corradi, Francesco; Zaccaria, Andrea; Pietronero, Luciano

    2015-12-01

    We present an empirical analysis of the microstructure of financial markets and, in particular, of the static and dynamic properties of liquidity. We find that on relatively large time scales (15 min) large price fluctuations are connected to the failure of the subtle mechanism of compensation between the flows of market and limit orders: in other words, the missed revelation of the latent order book breaks the dynamical equilibrium between the flows, triggering the large price jumps. On smaller time scales (30 s), instead, the static depletion of the limit order book is an indicator of an intrinsic fragility of the system, which is related to a strongly nonlinear enhancement of the response. In order to quantify this phenomenon we introduce a measure of the liquidity imbalance present in the book and we show that it is correlated to both the sign and the magnitude of the next price movement. These findings provide a quantitative definition of the effective liquidity, which proves to be strongly dependent on the considered time scales.

  13. Time scales and structures of wave interaction

    CERN Document Server

    Kartashova, Elena

    2013-01-01

    In this paper we give a general account of Wave Interaction Theory which by now consists of two parts: kinetic wave turbulence theory (WTT), using a statistical description of wave interactions, and the D-model recently introduced in \\emph{Kartashova, PRE \\textbf{86}: 041129 (2012)} describing interactions of distinct modes. Applying time scale analysis to weakly nonlinear wave systems modeled by the focusing nonlinear Sch\\"{o}dinger equation, we give an overview of the structures appearing in Wave Interaction Theory, their time scales and characteristic times. We demonstrate that kinetic cascade and D-cascade are not competing processes but rather two processes taking place at different time scales, at different characteristic levels of nonlinearity and due to different physical mechanisms. Taking surface water waves as an example we show that energy cascades in this system occur at much faster characteristic times than those required by the kinetic WTT but can be described as D-cascades. As D-model has no s...

  14. Separation of small molecular peptides with the same amino acid composition but different sequences by high performance liquid chromatography-electrospray ionization-mass spectrometry

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Peptidomics has emerged as a new discipline in recent years. Mass spectrometry (MS) is the most universal and efficient tool for structure identification of proteins and peptides. However,there is a limitation for the identification of peptides with the same amino acid composition but different se-quences because these peptides have identical mass spectra of molecular ions. This paper presents a high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) method for the separation of small molecular peptides with the same amino acid composition but dif-ferent sequences. Two tripeptides of Gly-Ser-Phe and Gly-Phe-Ser were used as a model sample. The separation behavior has been investigated and the separation conditions have been optimized. Under the optimum conditions,good repeatability was achieved. The developed method could provide a helpful reference for the separation of other peptides with the same amino acid composition but different sequences in the study of proteomics and peptidomics.

  15. Peptide profiling of Internet-obtained Cerebrolysin using high performance liquid chromatography - electrospray ionization ion trap and ultra high performance liquid chromatography - ion mobility - quadrupole time of flight mass spectrometry.

    Science.gov (United States)

    Gevaert, Bert; D'Hondt, Matthias; Bracke, Nathalie; Yao, Han; Wynendaele, Evelien; Vissers, Johannes Petrus Cornelis; De Cecco, Martin; Claereboudt, Jan; De Spiegeleer, Bart

    2015-09-01

    Cerebrolysin, a parenteral peptide preparation produced by controlled digestion of porcine brain proteins, is an approved nootropic medicine in some countries. However, it is also easily and globally available on the Internet. Nevertheless, until now, its exact chemical composition was unknown. Using high performance liquid chromatography (HPLC) coupled to ion trap and ultra high performance liquid chromatography (UHPLC) coupled to quadrupole-ion mobility-time-of-flight mass spectrometry (Q-IM-TOF MS), combined with UniProt pig protein database search and PEAKS de novo sequencing, we identified 638 unique peptides in an Internet-obtained Cerebrolysin sample. The main components in this sample originate from tubulin alpha- and beta-chain, actin, and myelin basic protein. No fragments of known neurotrophic factors like glial cell-derived neurotrophic factor (GDNF), neurotrophin nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF) were found, suggesting that the activities reported in the literature are likely the result of new, hitherto unknown cryptic peptides with nootropic properties. PMID:26017115

  16. Peptide profiling of Internet-obtained Cerebrolysin using high performance liquid chromatography - electrospray ionization ion trap and ultra high performance liquid chromatography - ion mobility - quadrupole time of flight mass spectrometry.

    Science.gov (United States)

    Gevaert, Bert; D'Hondt, Matthias; Bracke, Nathalie; Yao, Han; Wynendaele, Evelien; Vissers, Johannes Petrus Cornelis; De Cecco, Martin; Claereboudt, Jan; De Spiegeleer, Bart

    2015-09-01

    Cerebrolysin, a parenteral peptide preparation produced by controlled digestion of porcine brain proteins, is an approved nootropic medicine in some countries. However, it is also easily and globally available on the Internet. Nevertheless, until now, its exact chemical composition was unknown. Using high performance liquid chromatography (HPLC) coupled to ion trap and ultra high performance liquid chromatography (UHPLC) coupled to quadrupole-ion mobility-time-of-flight mass spectrometry (Q-IM-TOF MS), combined with UniProt pig protein database search and PEAKS de novo sequencing, we identified 638 unique peptides in an Internet-obtained Cerebrolysin sample. The main components in this sample originate from tubulin alpha- and beta-chain, actin, and myelin basic protein. No fragments of known neurotrophic factors like glial cell-derived neurotrophic factor (GDNF), neurotrophin nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF) were found, suggesting that the activities reported in the literature are likely the result of new, hitherto unknown cryptic peptides with nootropic properties.

  17. Simple setup for gas-phase h/d exchange mass spectrometry coupled to electron transfer dissociation and ion mobility for analysis of polypeptide structure on a liquid chromatographic time scale

    DEFF Research Database (Denmark)

    Mistarz, Ulrik Hvid; Brown, Jeffery M; Haselmann, Kim F;

    2014-01-01

    with liquid chromatography and a chip-based automated nanoESI interface, allowing for online gas-phase HDX-MS analysis of peptides and proteins separated on a liquid chromatographic time scale at increased throughput. Furthermore, online gas-phase HDX-MS could be performed in tandem with ion mobility......Gas-phase hydrogen/deuterium exchange (HDX) is a fast and sensitive, yet unharnessed analytical approach for providing information on the structural properties of biomolecules, in a complementary manner to mass analysis. Here, we describe a simple setup for ND3-mediated millisecond gas-phase HDX...

  18. Isolation of cross-linked peptides by diagonal strong cation exchange chromatography for protein complex topology studies by peptide fragment fingerprinting from large sequence databases.

    Science.gov (United States)

    Buncherd, Hansuk; Roseboom, Winfried; Ghavim, Behrad; Du, Weina; de Koning, Leo J; de Koster, Chris G; de Jong, Luitzen

    2014-06-27

    Knowledge of spatial proximity of amino acid residues obtained by chemical cross-linking and mass spectrometric analysis provides information about protein folding, protein-protein interactions and topology of macromolecular assemblies. We show that the use of bis(succinimidyl)-3-azidomethyl glutarate as a cross-linker provides a solution for two major analytical problems of cross-link mapping by peptide fragment fingerprinting (PFF) from complex sequence databases, i.e., low abundance of protease-generated target peptides and lack of knowledge of the masses of linked peptides. Tris(carboxyethyl)phosphine (TCEP) reduces the azido group in cross-linked peptides to an amine group in competition with cleavage of an amide bond formed in the cross-link reaction. TCEP-induced reaction products were separated by diagonal strong cation exchange (SCX) from unmodified peptides. The relation between the sum of the masses of the cleavage products and the mass of the parent cross-linked peptide enables determination of the masses of candidate linked peptides. By reversed phase LC-MS/MS analysis of secondary SCX fractions, we identified several intraprotein and interprotein cross-links in a HeLa cell nuclear extract, aided by software tools supporting PFF from the entire human sequence database. The data provide new information about interacting protein domains, among others from assemblies involved in splicing.

  19. Short-time scale behavior modeling within long-time scale fuel cycle evaluations

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, M.; Tsvetkov, P. [Dept. of Nuclear Engineering, Texas A and M Univ., 3133 TAMU, College Station, TX 77843 (United States); Lucas, S. [Idaho National Laboratory, P.O. Box 1625, Idaho Falls, ID 83415 (United States)

    2012-07-01

    Typically, short-time and long-time scales in nuclear energy system behavior are accounted for with entirely separate models. However, long-term changes in system characteristics do affect short-term transients through material variations. This paper presents an approach to consistently account for short-time scales within a nuclear system lifespan. The reported findings and developments are of significant importance for small modular reactors and other nuclear energy systems operating in autonomous modes. It is necessary to simulate the short time-scale kinetic behavior of the reactor as well as the long time-scale dynamics that occur with fuel burnup. The former is modeled using the point kinetics equations, while the latter is modeled by the Bateman equations. (authors)

  20. Quantum time scales in alpha tunneling

    CERN Document Server

    Kelkar, N G; Nowakowski, M

    2008-01-01

    The theoretical treatment of alpha decay by Gamow is revisited by investigating the quantum time scales in tunneling. The time spent by an alpha particle in front of the barrier and traversing it before escape is evaluated using microscopic alpha nucleus potentials. The half-life of a nucleus is shown to correspond to the time spent by the alpha knocking in front of the barrier. Calculations for medium and super heavy nuclei show that from a multitude of available tunneling time definitions, the transmission dwell time gives the bulk of the lifetime of the decaying state, in most cases.

  1. Short communication: Identification of iron-binding peptides from whey protein hydrolysates using iron (III)-immobilized metal ion affinity chromatography and reversed phase-HPLC-tandem mass spectrometry.

    Science.gov (United States)

    Cruz-Huerta, Elvia; Martínez Maqueda, Daniel; de la Hoz, Lucia; da Silva, Vera S Nunes; Pacheco, Maria Teresa Bertoldo; Amigo, Lourdes; Recio, Isidra

    2016-01-01

    Peptides with iron-binding capacity obtained by hydrolysis of whey protein with Alcalase (Novozymes, Araucaria, PR, Brazil), pancreatin, and Flavourzyme (Novozymes) were identified. Hydrolysates were subjected to iron (III)-immobilized metal ion affinity chromatography, and the bound peptides were sequenced by mass spectrometry. Regardless of the enzyme used, the domains f(42-59) and f(125-137) from β-lactoglobulin enclosed most of identified peptides. This trend was less pronounced in the case of peptides derived from α-lactalbumin, with sequences deriving from diverse regions. Iron-bound peptides exhibited common structural characteristics, such as an abundance of Asp, Glu, and Pro, as revealed by mass spectrometry and AA analysis. In conclusion, this characterization of iron-binding peptides helps clarify the relationship between peptide structure and iron-chelating activity and supports the promising role of whey protein hydrolysates as functional ingredients in iron supplementation treatments. PMID:26601589

  2. [Determination of peptide and protein diversity in venom of the spider Selenocosmia jiafu by high performance liquid chromatography and mass spectrometry].

    Science.gov (United States)

    Hu, Zhaotun; Xiao, Zhen; Zhou, Xi; Chen, Jia; Chen, Bo; Liu, Zhonghua

    2015-06-01

    Selenocosmia jiafu (S. jiafu) is recently identified as a new species of spider in P. R. China. These medium bodied venomous spiders are distributed mainly in the hilly areas of southwest of China, mostly at Yunnan and Guangxi Provinces. In order to understand the composition of the S. jiafu venom, we performed a preliminary analysis of this venom using reversed-phase high performance liquid chromatography (RP-HPLC), matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The S. jiafu venom was separated by RP-HPLC in an analytical C18 column (phenomenex 100 A, 250 mm x 4.6 mm, 5 µm) equilibrated with solution A (distilled water with 0.1% trifluoroacetic acid), using a gradient from 0% to 50% of solution B (acetonitrile with 0.1% trifluoroacetic acid) over 50 min with a flow rate of 1 mL/min. The isolated venom proteins were treated with in-gel digestion separated by SDS- PAGE and then identified by liquid chromatography-electrospray ionization quadrupole-time of flight mass spectrometry (LC-ESI-QTOF-MS) techniques. The results show that more than 40 fractions eluted were monitored at 215 nm in the RP-HPLC chromatogram of the venom of the spider S. jiafu. Most of the fractions were eluted with retention times of 5-15 min and 25-40 min, corresponding to 5%-15% and 25%-40% acetonitrile, respectively. The venom contains 238 peptides that follow a bimodal distribution, with about 62.5% of the peptides having a relative molecular mass of 3,000-4,500 and about 33.2% of the peptides having a relative molecular mass of 1,000-3,000. This distribution model is rather different from those of peptides from other tarantula spider venoms analyzed. To explore the relative molecular mass distribution of the venom proteins, the venom was analyzed by SDS-PAGE using standard protocols. Except for peptides with relative molecular mass lower than 10,000, the SDS

  3. Liquid chromatography-electrospray linear ion trap mass spectrometry analysis of targeted neuropeptides in Tac1(-/-) mouse spinal cords reveals significant lower concentration of opioid peptides.

    Science.gov (United States)

    Saidi, Mouna; Beaudry, Francis

    2015-08-01

    Tachykinin and opioid peptides play a central role in pain transmission, modulation and inhibition. The treatment of pain is very important in medicine and many studies using NK1 receptor antagonists failed to show significant analgesic effects in humans. Recent investigations suggest that both pronociceptive tachykinins and the analgesic opioid systems are important for normal pain sensation. The analysis of opioid peptides in Tac1(-/-) spinal cord tissues offers a great opportunity to verify the influence of the tachykinin system on specific opioid peptides. The objectives of this study were to develop an HPLC-MS/MRM assay to quantify targeted peptides in spinal cord tissues. Secondly, we wanted to verify if the Tac1(-/-) mouse endogenous opioid system is hampered and therefore affects significantly the pain modulatory pathways. Targeted neuropeptides were analyzed by high performance liquid chromatography linear ion trap mass spectrometry. Our results reveal that EM-2, Leu-Enk and Dyn A were down-regulated in Tac1(-/-) spinal cord tissues. Interestingly, Dyn A was almost 3 fold down-regulated (p<0.0001). No significant concentration differences were observed in mouse Tac1(-/-) spinal cords for Met-Enk and CGRP. The analysis of Tac1(-/-) mouse spinal cords revealed noteworthy decreases of EM-2, Leu-Enk and Dyn A concentrations which strongly suggest a significant impact on the endogenous pain-relieving mechanisms. These observations may have insightful impact on future analgesic drug developments and therapeutic strategies.

  4. Identification of bradykinin: related peptides from Phyllomedusa nordestina skin secretion using electrospray ionization tandem mass spectrometry after a single-step liquid chromatography

    Directory of Open Access Journals (Sweden)

    K Conceição

    2009-01-01

    Full Text Available Amphibian skin secretions are a source of potential new drugs with medical and biotechnological applications. Rich in peptides produced by holocrine-type serous glands in the integument, these secretions play different roles, either in the regulation of physiological skin functions or in the defense against predators or microorganisms. The aim of the present work was to identify novel peptides with bradykinin-like structure and/or activity present in the skin of Phyllomedusa nordestina. In order to achieve this goal, the crude skin secretion of this frog was pre-fractionated by solid phase extraction and separated by reversed-phase chromatography. The fractions were screened for low-molecular-mass peptides and sequenced by mass spectrometry. It was possible to identify three novel bradykinin-related peptides, namely: KPLWRL-NH2 (Pnor 3, RPLSWLPK (Pnor 5 and VPPKGVSM (Pnor 7 presenting vascular activities as assessed by intravital microscopy. Pnor 3 and Pnor 7 were able to induce vasodilation. On the other hand, Pnor 5 was a potent vasoconstrictor. These effects were reproduced by their synthetic analogues.

  5. Near infrared spectroscopy compared to liquid chromatography coupled to mass spectrometry and capillary electrophoresis as a detection tool for peptide reaction monitoring.

    Science.gov (United States)

    Petter, Christine H; Heigl, Nico; Bachmann, Stefan; Huck-Pezzei, Verena A C; Najam-Ul-Haq, Muhammad; Bakry, Rania; Bernkop-Schnürch, Andreas; Bonn, Günther K; Huck, Christian W

    2008-05-01

    Peptide interaction is normally monitored by liquid chromatography (LC), liquid chromatography coupled to mass spectrometry (LC-MS), mass spectrometric (MS) methods such as MALDI-TOF/MS or capillary electrophoresis (CE). These analytical techniques need to apply either high pressure or high voltages, which can cause cleavage of newly formed bondages. Therefore, near infrared reflectance spectroscopy (NIRS) is presented as a rapid alternative to monitor the interaction of glutathione and oxytocin, simulating physiological conditions. Thereby, glutathione can act as a nucleophile with oxytocin forming four new conjugates via a disulphide bondage. Liquid chromatography coupled to UV (LC-UV) and mass spectrometry via an electrospray ionisation interface (LC-ESI-MS) resulted in a 82% and a 78% degradation of oxytocin at pH 3 and a 5% and a 7% degradation at pH 6.5. Capillary electrophoresis employing UV-detection (CE-UV) showed a 44% degradation of oxytocin. LC and CE in addition to the NIRS are found to be authentic tools for quantitative analysis. Nevertheless, NIRS proved to be highly suitable for the detection of newly formed conjugates after separating them on a thin layer chromatography (TLC) plate. The recorded fingerprint in the near infrared region allows for a selective distinct qualitative identification of conjugates without the need for expensive instrumentation such as quadrupole or MALDI-TOF mass spectrometers. The performance of the established NIRS method is compared to LC and CE; its advantages are discussed in detail. PMID:18095054

  6. Various Time-Scales of Relaxation

    CERN Document Server

    Ali-Akbari, M; Ebrahim, H; Shahkarami, L

    2016-01-01

    Via gauge-gravity duality, relaxation of far-from-equilibrium initial states in a strongly coupled gauge theory has been investigated. In the system we consider in this paper there are two ways where the state under study can deviate from its equilibrium: anisotropic pressure and time-dependent expectation value of a scalar operator with \\Delta=2. In the gravity theory, this system corresponds to Einstein's general relativity with a non-trivial metric, including the anisotropy function, coupled to a massive scalar matter field. We study the effect of different initial configurations for scalar field and anisotropy function on physical processes such as thermalization, i.e. time evolution of event horizon, equilibration of the expectation value of scalar operator and isotropization. We also discuss time ordering of these time-scales.

  7. Discounting in Games across Time Scales

    Directory of Open Access Journals (Sweden)

    Krishnendu Chatterjee

    2010-06-01

    Full Text Available We introduce two-level discounted games played by two players on a perfect-information stochastic game graph. The upper level game is a discounted game and the lower level game is an undiscounted reachability game. Two-level games model hierarchical and sequential decision making under uncertainty across different time scales. We show the existence of pure memoryless optimal strategies for both players and an ordered field property for such games. We show that if there is only one player (Markov decision processes, then the values can be computed in polynomial time. It follows that whether the value of a player is equal to a given rational constant in two-level discounted games can be decided in NP intersected coNP. We also give an alternate strategy improvement algorithm to compute the value.

  8. Discounting in Games across Time Scales

    CERN Document Server

    Chatterjee, Krishnendu; 10.4204/EPTCS.25.6

    2010-01-01

    We introduce two-level discounted games played by two players on a perfect-information stochastic game graph. The upper level game is a discounted game and the lower level game is an undiscounted reachability game. Two-level games model hierarchical and sequential decision making under uncertainty across different time scales. We show the existence of pure memoryless optimal strategies for both players and an ordered field property for such games. We show that if there is only one player (Markov decision processes), then the values can be computed in polynomial time. It follows that whether the value of a player is equal to a given rational constant in two-level discounted games can be decided in NP intersected coNP. We also give an alternate strategy improvement algorithm to compute the value.

  9. Extension of gyrokinetics to transport time scales

    CERN Document Server

    Parra, Felix I

    2013-01-01

    Gyrokinetic simulations have greatly improved our theoretical understanding of turbulent transport in fusion devices. Most gyrokinetic models in use are delta-f simulations in which the slowly varying radial profiles of density and temperature are assumed to be constant for turbulence saturation times, and only the turbulent electromagnetic fluctuations are calculated. New massive simulations are being built to self-consistently determine the radial profiles of density and temperature. However, these new codes have failed to realize that modern gyrokinetic formulations, composed of a gyrokinetic Fokker-Planck equation and a gyrokinetic quasineutrality equation, are only valid for delta-f simulations that do not reach the longer transport time scales necessary to evolve radial profiles. In tokamaks, due to axisymmetry, the evolution of the axisymmetric radial electric field is a challenging problem requiring substantial modifications to gyrokinetic treatments. In this thesis, I study the effect of turbulence o...

  10. Analysis of aspartyl peptide degradation products by high-performance liquid chromatography and high-performance liquid chromatography-mass spectrometry.

    Science.gov (United States)

    De Boni, Silvia; Oberthür, Christine; Hamburger, Matthias; Scriba, Gerhard K E

    2004-01-01

    A reversed-phase HPLC method for the analysis of degradation products of the model aspartyl tripeptides Phe-Asp-GlyNH2 and Gly-Asp-PheNH2 after incubation at pH 2 and 10 was developed. Most of the compounds could be separated with a gradient of acetonitrile in water containing 0.1% trifluoroacetic acid. Resolution of the isomeric pairs L-Phe-alpha-L-Asp-GlyNH2/L-Phe-beta-L-Asp-GlyNH2 and L-Phe-alpha-D-Asp-GlyOH/L-Phe-beta-D-Asp-GlyOH was achieved with a gradient of acetonitrile in phosphate buffer, pH 5.0. Under acidic conditions the major degradation pathway was cleavage of the peptide backbone amide bonds yielding dipeptides and amino acids, C-terminal deamidation as well as formation of succidinimyl peptides. At alkaline pH both deamidation of the C-terminal amide as well as isomerization and concomitant enantiomerization of Asp were observed. The peaks were identified both by reference substances and by online electrospray mass spectrometry. The results were compared to a previous developed capillary electrophoresis method. Diastereomeric pairs ofpeptides that could not be separated by capillary electrophoresis were resolved by HPLC while the separation of corresponding pairs of alpha- and beta-Asp peptides was not always achieved by HPLC in contrast to capillary electrophoresis illustrating that both techniques can be complimentary in peptide analysis. PMID:14753775

  11. Potential Therapeutic Applications of Mucuna pruriens Peptide Fractions Purified by High-Performance Liquid Chromatography as Angiotensin-Converting Enzyme Inhibitors, Antioxidants, Antithrombotic and Hypocholesterolemic Agents.

    Science.gov (United States)

    Herrera-Chalé, Francisco; Ruiz-Ruiz, Jorge Carlos; Betancur-Ancona, David; Segura-Campos, Maira Rubi

    2016-02-01

    A Mucuna pruriens protein concentrate was hydrolyzed with a digestive (pepsin-pancreatin) enzymatic system. The soluble portion of the hydrolysate was fractionated by ultrafiltration and the ultrafiltered peptide fraction (PF) with lower molecular weight was purified by reversed-phase high-performance liquid chromatography. The PF obtained were evaluated by testing the biological activity in vitro. Fractions showed that the ability to inhibit the angiotensin-converting enzyme had IC50 values that ranged from 2.7 to 6.2 μg/mL. Trolox equivalent antioxidant capacity values ranged from 132.20 to 507.43 mM/mg. The inhibition of human platelet aggregation ranged from 1.59% to 11.11%, and the inhibition of cholesterol micellar solubility ranged from 0.24% to 0.47%. Hydrophobicity, size, and amino acid sequence could be factors in determining the biological activity of peptides contained in fractions. This is the first report that M. pruriens peptides act as antihypertensives, antioxidants, and inhibitors for human platelet aggregation and cholesterol micellar solubility in vitro. PMID:26854846

  12. Homoclinical Structure of Dynamic Equations on Time Scales

    OpenAIRE

    Fen, Mehmet Onur

    2016-01-01

    Homoclinic and heteroclinic motions in dynamics equations on time scales is investigated. The utilized time scale is a specific one such that it is a union of disjoint compact intervals. A numerical example that supports the theoretical results is presented.

  13. Solution-phase-peptide synthesis via the Group-Assisted Purification (GAP) chemistry without using chromatography and recrystallization†

    OpenAIRE

    Wu, Jianbin; An, Guanghui; Lin, Siqi; Xie, Jianbo; Zhou, Wei; Sun, Hao; Pan, Yi; Li, Guigen

    2014-01-01

    The solution phase synthesis of N-protected amino acids and peptides has been achieved through the Group-Assisted Purification (GAP) chemistry by avoiding disadvantages of other methods in regard to the difficult scale-up, expenses of solid and soluble polymers, etc. The GAP synthesis can reduce the use of solvents, silica gels, energy and manpower. In addition, the GAP auxiliary can be conveniently recovered for re-use and is of environmentally friendly benign by substantially reducing waste...

  14. Normality of Ethernet Traffic at Large Time Scales

    Directory of Open Access Journals (Sweden)

    Zhiping Lu

    2013-01-01

    Full Text Available We contribute the quantitative descriptions of the large time scales for the Ethernet traffic to be Gaussian. We focus on the normality property of the accumulated traffic data under different time scales. The investigation is carried out graphically by the quantile-quantile (QQ plots and numerically by statistical tests. The present results indicate that the larger the time scale, the more normal the Ethernet traffic.

  15. Multiple Time Scales and Longitudinal Measurements in Event History Analysis

    OpenAIRE

    Danardono,

    2005-01-01

    A general time-to-event data analysis known as event history analysis is considered. The focus is on the analysis of time-to-event data using Cox's regression model when the time to the event may be measured from different origins giving several observable time scales and when longitudinal measurements are involved. For the multiple time scales problem, procedures to choose a basic time scale in Cox's regression model are proposed. The connections between piecewise constant hazards, time-depe...

  16. Solution-phase-peptide synthesis via the group-assisted purification (GAP) chemistry without using chromatography and recrystallization.

    Science.gov (United States)

    Wu, Jianbin; An, Guanghui; Lin, Siqi; Xie, Jianbo; Zhou, Wei; Sun, Hao; Pan, Yi; Li, Guigen

    2014-02-01

    The solution phase synthesis of N-protected amino acids and peptides has been achieved through the Group-Assisted Purification (GAP) chemistry by avoiding disadvantages of other methods in regard to the difficult scale-up, expenses of solid and soluble polymers, etc. The GAP synthesis can reduce the use of solvents, silica gels, energy and manpower. In addition, the GAP auxiliary can be conveniently recovered for re-use and is environmentally friendly and benign, and substantially reduces waste production in academic labs and industry.

  17. A new generalization of Ostrowski type inequality on time scales

    CERN Document Server

    Liu, Wenjun

    2008-01-01

    In this paper we first extend a generalization of Ostrowski type inequality on time scales for functions whose derivatives are bounded and then unify corresponding continuous and discrete versions. We also point out some particular integral type inequalities on time scales as special cases.

  18. Bounds of Certain Dynamic Inequalities on Time Scales

    Directory of Open Access Journals (Sweden)

    Deepak B. Pachpatte

    2014-10-01

    Full Text Available In this paper we study explicit bounds of certain dynamic integral inequalities on time scales. These estimates give the bounds on unknown functions which can be used in studying the qualitative aspects of certain dynamic equations. Using these inequalities we prove the uniqueness of some partial integro-differential equations on time scales.

  19. Peptide sequencing and characterization of post-translational modifications by enhanced ion-charging and liquid chromatography electron-transfer dissociation tandem mass spectrometry

    DEFF Research Database (Denmark)

    Kjeldsen, Frank; Giessing, Anders; Ingrell, Christian R;

    2007-01-01

    system coupled to an ESI tandem mass spectrometer. Addition of just 0.1% m-NBA changed the average charge state for the identified tryptic BSA peptides from 2.2+ to 2.6+. As a result, the predominant charge states for BSA peptides were changed from 2+ to > or =3+. To evaluate the benefits of peptide...... charge enhancement, the ETD fragmentation efficiency and Mascot peptide score were compared for BSA peptides in charge states 2+ and 3+. In all cases but one, triply charged peptides fragmented more efficiently than the analogues 2+ peptide ions. On average, triply charged peptides received a 68% higher...

  20. Objective quality measurement for audio time-scale modification

    Science.gov (United States)

    Liu, Fang; Lee, Jae-Joon; Kuo, C. C. J.

    2003-11-01

    The recent ITU-T Recommendation P.862, known as the Perceptual Evaluation of Speech Quality (PESQ) is an objective end-to-end speech quality assessment method for telephone networks and speech codecs through the measurement of received audio quality. To ensure that certain network distortions will not affect the estimated subjective measurement determined by PESQ, the algorithm takes into account packet loss, short-term and long-term time warping resulted from delay variation. However, PESQ does not work well for time-scale audio modification or temporal clipping. We investigated the factors that impact the perceived quality when time-scale modification is involved. An objective measurement of time-scale modification is proposed in this research, where the cross-correlation values obtained from time-scale modification synchronization are used to evaluate the quality of a time-scaled audio sequence. This proposed objective measure has been verified by a subjective test.

  1. Temperature dependence of fluctuation time scales in spin glasses

    DEFF Research Database (Denmark)

    Kenning, Gregory G.; Bowen, J.; Sibani, Paolo;

    2010-01-01

    Using a series of fast cooling protocols we have probed aging effects in the spin glass state as a function of temperature. Analyzing the logarithmic decay found at very long time scales within a simple phenomenological barrier model, leads to the extraction of the fluctuation time scale of the s......-aging behavior in scaling aged TRM decay curves, as recently discussed theoretically~\\cite{Sibani09}.......Using a series of fast cooling protocols we have probed aging effects in the spin glass state as a function of temperature. Analyzing the logarithmic decay found at very long time scales within a simple phenomenological barrier model, leads to the extraction of the fluctuation time scale of the...... system at a particular temperature. This is the smallest dynamical time-scale, defining a lower-cut off in a hierarchical description of the dynamics.  We find that this fluctuation time scale, which is approximately equal to atomic spin fluctuation time scales near the transition temperature, follows a...

  2. Absolute quantification of Pru av 2 in sweet cherry fruit by liquid chromatography/tandem mass spectrometry with the use of a stable isotope-labelled peptide.

    Science.gov (United States)

    Ippoushi, Katsunari; Sasanuma, Motoe; Oike, Hideaki; Kobori, Masuko; Maeda-Yamamoto, Mari

    2016-08-01

    Pru av 2, a pathogenesis-related (PR) protein present in the sweet cherry (Prunus avium L.) fruit, is the principal allergen of cherry and one of the chief causes of pollen food syndrome (oral allergy syndrome). In this study, a quantitative assay for this protein was developed with the use of the protein absolute quantification (AQUA) method, which consists of liquid chromatography/tandem mass spectrometry (LC/MS/MS) employing TGC[CAM]STDASGK[(13)C6,(15)N2], a stable isotope-labelled internal standard (SIIS) peptide. This assay gave a linear relationship (r(2)>0.99) in a concentration range (2.3-600fmol/μL), and the overall coefficient of variation (CV) for multiple tests was 14.6%. Thus, the contents of this allergenic protein in sweet cherry products could be determined using this assay. This assay should be valuable for allergological investigations of Pru av 2 in sweet cherry and detection of protein contamination in foods. PMID:26988485

  3. Rapid and specific high-performance liquid chromatography for the in vitro quantification of D-Lys6-GnRH in a microemulsion-type formulation in the presence of peptide oxidation products.

    Science.gov (United States)

    Kafka, Alexandra P; Rades, Thomas; McDowell, Arlene

    2010-02-01

    A high-performance liquid chromatography (HPLC) method for assay of d-Lys(6)-GnRH contained in a microemulsion-type formulation is described. The peptide is extracted from the microemulsion matrix and quantified using a two-step gradient method. Separation from microemulsion compounds and potential peptide oxidation products was achieved on a Jupiter C(18) column at 40 degrees C, using a gradient of 10-35% CH(3)CN for peptide elution. The correlation of peak intensity measured at 220 nm and peptide concentration was linear over the range 2.5-60 microg/mL with a correlation coefficient of 0.9997 and a y-intercept not significantly different from zero (p > 0.05). Intraday and interday variability of the assay was less than 5% for multiple injections of samples containing 7.5, 30 and 60 microg/mL. The lower limit of quantitation was calculated to be 0.38 microg/mL, and the lower limit of detection was 0.13 microg/mL. The assay was applied to samples that were stressed under physiological conditions (37 degrees C, pH 7.4) over 4 days. Three degradation peaks were well resolved from the parent peptide, demonstrating the selectivity of the assay. Off-line MALDI TOF mass spectrometry was applied to identify these degradation species as oxidation products of the peptide.

  4. A remark for dynamic equations on time scales

    OpenAIRE

    Akhmet, Marat; TURAN, Mehmet

    2010-01-01

    We give a proposal to generalize the concept of the differential equations on time scales, such that they can be more appropriate for the analysis of real world problems, and give more opportunities to increase the theoretical depth of investigation.

  5. The limit order book on different time scales

    CERN Document Server

    Eisler, Zoltan; Lillo, Fabrizio

    2007-01-01

    Financial markets can be described on several time scales. We use data from the limit order book of the London Stock Exchange (LSE) to compare how the fluctuation dominated microstructure crosses over to a more systematic global behavior.

  6. BOUNDARY VALUE PROBLEM TO DYNAMIC EQUATION ON TIME SCALE

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    In this paper we consider a nonlinear first-order boundary value problem on a time scale. The existence results of three positive solutions are obtained using fixed point theorems. Finally,examples are presented to illustrate the main results.

  7. Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, HCD and ETD-MS applied to the N-linked glycoproteome of Campylobacter jejuni

    DEFF Research Database (Denmark)

    Scott, Nichollas E; Parker, Benjamin L; Connolly, Angela M;

    2011-01-01

    -linked glycosylation sites in C. jejuni and is the first to utilize HCD fragmentation for glycopeptide identification with intact glycan. We also show that hydrophobic integral membrane proteins are significant targets of glycosylation in this organism. Our data demonstrate that peptide-centric approaches coupled...... by the resistance of the glycan-peptide bond to enzymatic digestion or ss-elimination, and have previously concentrated on soluble glycoproteins compatible with lectin affinity and gel-based approaches. We developed strategies for enriching C. jejuni HB93-13 glycopeptides using zwitterionic hydrophilic interaction...... chromatography (ZIC-HILIC) and examined novel fragmentation, including collision-induced dissociation (CID) and higher energy collisional (C-trap) dissociation (HCD), as well as CID / electron transfer dissociation (ETD) mass spectrometry. CID/HCD enabled the identification of glycan structure and peptide...

  8. Hydrodynamic Time Scales and Temporal Structure in GRBs

    OpenAIRE

    Sari, Re'em; Piran, Tsvi

    1995-01-01

    We calculate the hydrodynamic time scales for a spherical ultra-relativistic shell that is decelerated by the ISM and discuss the possible relations between these time scales and the observed temporal structure in $\\gamma$-ray bursts. We suggest that the bursts' duration is related to the deceleration time, the variability is related to the ISM inhomogeneities and precursors are related to internal shocks within the shell. Good agreement can be achieved for these quantities with reasonable, n...

  9. Controllability of multiplex, multi-time-scale networks

    Science.gov (United States)

    Pósfai, Márton; Gao, Jianxi; Cornelius, Sean P.; Barabási, Albert-László; D'Souza, Raissa M.

    2016-09-01

    The paradigm of layered networks is used to describe many real-world systems, from biological networks to social organizations and transportation systems. While recently there has been much progress in understanding the general properties of multilayer networks, our understanding of how to control such systems remains limited. One fundamental aspect that makes this endeavor challenging is that each layer can operate at a different time scale; thus, we cannot directly apply standard ideas from structural control theory of individual networks. Here we address the problem of controlling multilayer and multi-time-scale networks focusing on two-layer multiplex networks with one-to-one interlayer coupling. We investigate the practically relevant case when the control signal is applied to the nodes of one layer. We develop a theory based on disjoint path covers to determine the minimum number of inputs (Ni) necessary for full control. We show that if both layers operate on the same time scale, then the network structure of both layers equally affect controllability. In the presence of time-scale separation, controllability is enhanced if the controller interacts with the faster layer: Ni decreases as the time-scale difference increases up to a critical time-scale difference, above which Ni remains constant and is completely determined by the faster layer. We show that the critical time-scale difference is large if layer I is easy and layer II is hard to control in isolation. In contrast, control becomes increasingly difficult if the controller interacts with the layer operating on the slower time scale and increasing time-scale separation leads to increased Ni, again up to a critical value, above which Ni still depends on the structure of both layers. This critical value is largely determined by the longest path in the faster layer that does not involve cycles. By identifying the underlying mechanisms that connect time-scale difference and controllability for a simplified

  10. Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry

    Directory of Open Access Journals (Sweden)

    Min Jung Kim

    2013-01-01

    Full Text Available The present study aimed to identify key metabolites related to weight reduction in humans by studying the metabolic profiles of sera obtained from 34 participants who underwent dietary intervention with black soybean peptides (BSP for 12 weeks. This research is a sequel to our previous work in which the effects of BSP on BMI and blood composition of lipid were investigated. Sera of the study were subjected to ultra performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS, and the data were analyzed using partial least-squares discriminate analysis (PLS-DA score plots. Body mass index and percent body fat of the test group were reduced. Levels of betaine, benzoic acid, pyroglutamic acid, pipecolic acid, N-phenylacetamide, uric acid, l-aspartyl-l-phenylalanine, and lysophosphatidyl cholines (lysoPCs (C18:1, C18:2, C20:1, and C20:4 showed significant increases. Levels of l-proline, valine, l-leucine/isoleucine, hypoxanthine, glutamine, l-methionine, phenylpyruvic acid, several carnitine derivatives, and lysoPCs (C14:0, PC16:0, C15:0, C16:0, C17:1, C18:0, and C22:0 were significantly decreased. In particular, lysoPC 16:0 with a VIP value of 12.02 is esteemed to be the most important metabolite for evaluating the differences between the 2 serum samples. Our result confirmed weight-lowering effects of BSP, accompanied by favorable changes in metabolites in the subjects’ blood. Therefore, this research enables us to better understand obesity and increases the predictability of the obesity-related risk by studying metabolites present in the blood.

  11. Time scales and species coexistence in chaotic flows

    CERN Document Server

    Galla, Tobias

    2016-01-01

    Empirical observations in marine ecosystems have suggested a balance of biological and advection time scales as a possible explanation of species coexistence. To characterise this scenario, we measure the time to fixation in neutrally evolving populations in chaotic flows. Contrary to intuition the variation of time scales does not interpolate straightforwardly between the no-flow and well-mixed limits; instead we find that fixation is the slowest at intermediate Damk\\"ohler numbers, indicating long-lasting coexistence of species. Our analysis shows that this slowdown is due to spatial organisation on an increasingly modularised network. We also find that diffusion can either slow down or speed up fixation, depending on the relative time scales of flow and evolution.

  12. Wind power impacts and electricity storage - a time scale perspective

    DEFF Research Database (Denmark)

    Hedegaard, Karsten; Meibom, Peter

    2012-01-01

    power penetration, wind power impacts on different time scales are analysed. Results show consecutive negative and high net load period lengths indicating a significant potential for flexibility measures capable of charging/activating demand and discharging/inactivating demand in periods of 1 h to one......Integrating large amounts of wind power in energy systems poses balancing challenges due to the variable and only partly predictable nature of wind. The challenges cover different time scales from intra-hour, intra-day/day-ahead to several days and seasonal level. Along with flexible electricity...... demand options, various electricity storage technologies are being discussed as candidates for contributing to large-scale wind power integration and these also differ in terms of the time scales at which they can operate. In this paper, using the case of Western Denmark in 2025 with an expected 57% wind...

  13. Multiple time scales of fluvial processes—theory and applications

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Fluvial processes comprise water flow,sediment transport and bed evolution,which normally feature distinct time scales.The time scales of sediment transport and bed deformation relative to the flow essentially measure how fast sediment transport adapts to capacity region in line with local flow scenario and the bed deforms in comparison with the flow,which literally dictates if a capacity based and/or decoupled model is justified.This paper synthesizes the recently developed multiscale theory for sediment-l...

  14. Short—Time Scaling of Variable Ordering of OBDDs

    Institute of Scientific and Technical Information of China (English)

    龙望宁; 闵应骅; 等

    1997-01-01

    A short-time scaling criterion of variable ordering of OBDDs is proposed.By this criterion it is easy and fast to determine which one is better when several variable orders are given,especially when they differ 10% or more in resulted BDD size from each other.An adaptive variable order selection method,based on the short-time scaling criterion,is also presented.The experimental results show that this method is efficient and it makes the heuristic variable ordering methods more practical.

  15. Single-molecule binding experiments on long time scales

    NARCIS (Netherlands)

    Elenko, Mark P.; Szostak, Jack W.; van Oijen, Antoine M.

    2010-01-01

    We describe an approach for performing single-molecule binding experiments on time scales from hours to days, allowing for the observation of slower kinetics than have been previously investigated by single-molecule techniques. Total internal reflection fluorescence microscopy is used to image the b

  16. Holographic Brownian motion and time scales in strongly coupled plasmas

    NARCIS (Netherlands)

    A. Nata Atmaja; J. de Boer; M. Shigemori

    2010-01-01

    We study Brownian motion of a heavy quark in field theory plasma in the AdS/CFT setup and discuss the time scales characterizing the interaction between the Brownian particle and plasma constituents. In particular, the mean-free-path time is related to the connected 4-point function of the random fo

  17. Cognitive componets of speech at different time scales

    DEFF Research Database (Denmark)

    Feng, Ling; Hansen, Lars Kai

    2007-01-01

    have earlier been revealed at multiple time scales corresponding to: phoneme, gender, height and speaker identity. We here show that the same simple unsupervised learning algorithm can detect these cues. Our basic features are 25-dimensional short time Mel-frequency weighted cepstral coefficients...

  18. Speech Compensation for Time-Scale-Modified Auditory Feedback

    Science.gov (United States)

    Ogane, Rintaro; Honda, Masaaki

    2014-01-01

    Purpose: The purpose of this study was to examine speech compensation in response to time-scale-modified auditory feedback during the transition of the semivowel for a target utterance of /ija/. Method: Each utterance session consisted of 10 control trials in the normal feedback condition followed by 20 perturbed trials in the modified auditory…

  19. Multiple time scales in the microwave ionization of Rydberg atoms

    Energy Technology Data Exchange (ETDEWEB)

    Buchleitner, A.; Delande, D.; Zakrzewski, J.; Mantegna, R.N.; Arndt, M.; Walther, H. [Max-Planck-Institut fuer Quantenoptik, Hans-Kopfermann-Strasse 1, D-85748 Garching (Germany)]|[Laboratoire Kastler Brossel, 4 place Jussieu, Tour 12, 1 etage, F-75252 Paris Cedex 05 (France)]|[Instytut Fizyki Uniwersytetu Jagiellonskiego, ul. Reymonta 4, PL-30-059 Krakow (Poland)]|[Dipartimento di Energetica ed Applicazioni di Fisica, Universita di Palermo, Viale delle Scienze, I-90128 Palermo (Italy)]|[Sektion Physik der Universitaet Muenchen, Am Coulombwall 1, D-85748 Garching (Germany)

    1995-11-20

    We investigate the time dependence of the ionization probability of Rydberg atoms driven by microwave fields, both numerically and experimentally. Our exact quantum results provide evidence for an algebraic decay law on suitably chosen time scales, a phenomenon that is considered to be the signature of nonhyperbolic scattering in unbounded classically chaotic motion. {copyright} {ital 1995} {ital The} {ital American} {ital Physical} {ital Society}.

  20. Time Scale Calculus - a new perspectives for synthetic seismogram calculations

    Science.gov (United States)

    Waskiewicz, Kamil; Debski, Wojciech

    2013-04-01

    Synthetic, numerically generated seismograms are one of the key factors of any interpretation of recorded seismic data. At the early stage of development, calculation of full seismic waveforms was impossible due to a limited computational resource so we were forced to used only some selected characteristics of seismic waves relatively easy for numerical calculations like first arrival times, maximum amplitude, approximate source spectra, to name a few. Continues development of computational resources as well as progress in numerical techniques has opened possibilities of generation the full, 3-component seismograms incorporating many physically important elements like wave attenuation, anisotropy or randomness of the media. Although achieved results are impressive we still need new numerical methods to tackle existing problems with the synthetic seismogram generation. In this contribution we present a novel approach to discretization of the wave equation which brings together continues and discrete numerical analysis of the seismic waves. The foundations of this new technique, called Time Scale Calculus, have been formulated by Hilger in late eighties and is very dynamically developing. The Time scale calculus, due to its universality seems to have a great potential when practical applications are considered. Thus we have decided to bring the Time Scale calculus concept closer to geophysical, or more precisely to seismological applications. This presentation is intend as a basic introduction to the time scales calculus considered from seismological point of view. We shortly present and discuss the possibility of using the Time Scales (TS) technique for solving the simplest acoustic 2D wave equation keeping in mind its particular applications for mining induced seismicity.

  1. HMC algorithm with multiple time scale integration and mass preconditioning

    International Nuclear Information System (INIS)

    We present a variant of the HMC algorithm with mass preconditioning (Hasenbusch acceleration) and multiple time scale integration. We have tested this variant for standard Wilson fermions at β = 5.6 and at pion masses ranging from 380 MeV to 680 MeV. We show that in this situation its performance is comparable to the recently proposed HMC variant with domain decomposition as preconditioner. We give an update of the ''Berlin Wall'' figure, comparing the performance of our variant of the HMC algorithm to other published performance data. Advantages of the HMC algorithm with mass preconditioning and multiple time scale integration are that it is straightforward to implement and can be used in combination with a wide variety of lattice Dirac operators. (orig.)

  2. Realization of a time-scale with an optical clock

    CERN Document Server

    Grebing, C; Dörscher, S; Häfner, S; Gerginov, V; Weyers, S; Lipphardt, B; Riehle, F; Sterr, U; Lisdat, C

    2015-01-01

    Optical clocks are not only powerful tools for prime fundamental research, but are also deemed for the re-definition of the SI base unit second as they surpass the performance of caesium atomic clocks in both accuracy and stability by more than an order of magnitude. However, an important obstacle in this transition has so far been the limited reliability of the optical clocks that made a continuous realization of a time-scale impractical. In this paper, we demonstrate how this dilemma can be resolved and that a time-scale based on an optical clock can be established that is superior to one based on even the best caesium fountain clocks. The paper also gives further proof of the international consistency of strontium lattice clocks on the $10^{-16}$ accuracy level, which is another prerequisite for a change in the definition of the second.

  3. Exponentials and Laplace transforms on nonuniform time scales

    Science.gov (United States)

    Ortigueira, Manuel D.; Torres, Delfim F. M.; Trujillo, Juan J.

    2016-10-01

    We formulate a coherent approach to signals and systems theory on time scales. The two derivatives from the time-scale calculus are used, i.e., nabla (forward) and delta (backward), and the corresponding eigenfunctions, the so-called nabla and delta exponentials, computed. With these exponentials, two generalised discrete-time Laplace transforms are deduced and their properties studied. These transforms are compatible with the standard Laplace and Z transforms. They are used to study discrete-time linear systems defined by difference equations. These equations mimic the usual continuous-time equations that are uniformly approximated when the sampling interval becomes small. Impulse response and transfer function notions are introduced. This implies a unified mathematical framework that allows us to approximate the classic continuous-time case when the sampling rate is high or to obtain the standard discrete-time case, based on difference equations, when the time grid becomes uniform.

  4. Entropy Production of Nanosystems with Time Scale Separation

    Science.gov (United States)

    Wang, Shou-Wen; Kawaguchi, Kyogo; Sasa, Shin-ichi; Tang, Lei-Han

    2016-08-01

    Energy flows in biomolecular motors and machines are vital to their function. Yet experimental observations are often limited to a small subset of variables that participate in energy transport and dissipation. Here we show, through a solvable Langevin model, that the seemingly hidden entropy production is measurable through the violation spectrum of the fluctuation-response relation of a slow observable. For general Markov systems with time scale separation, we prove that the violation spectrum exhibits a characteristic plateau in the intermediate frequency region. Despite its vanishing height, the plateau can account for energy dissipation over a broad time scale. Our findings suggest a general possibility to probe hidden entropy production in nanosystems without direct observation of fast variables.

  5. The fission time scale measured with an atomic clock

    NARCIS (Netherlands)

    Kravchuk, VL; Wilschut, HW; Hunyadi, M; Kopecky, S; Lohner, H; Rogachevskiy, A; Siemssen, RH; Krasznahorkay, A; Hamilton, JH; Ramayya, AV; Carter, HK

    2003-01-01

    We present a new direct method of measuring the fission absolute time scale using an atomic clock based on the lifetime of a vacancy in the atomic K-shell. We studied the reaction Ne-20 + Th-232 -> O-16 + U-236* at 30 MeV/u. The excitation energy of about 115 MeV in such a reaction is in the range w

  6. MULTISCALE HOMOGENIZATION OF NONLINEAR HYPERBOLIC EQUATIONS WITH SEVERAL TIME SCALES

    Institute of Scientific and Technical Information of China (English)

    Jean Louis Woukeng; David Dongo

    2011-01-01

    We study the multiscale homogenization of a nonlinear hyperbolic equation in a periodic setting. We obtain an accurate homogenization result. We also show that as the nonlinear term depends on the microscopic time variable, the global homogenized problem thus obtained is a system consisting of two hyperbolic equations. It is also shown that in spite of the presence of several time scales, the global homogenized problem is not a reiterated one.

  7. Development of a capillary high performance liquid chromatography-ion trap-mass spectrometry method for the determination of VLIVP antihypertensive peptide in soybean crops.

    Science.gov (United States)

    Puchalska, Patrycja; García, M Concepción; Marina, M Luisa

    2014-04-18

    Soybean peptide VLIVP presents a very high antihypertensive activity (IC50 value 1.69μM), even higher than extensively studied IPP and VPP peptides from milk. Nevertheless, no much attention has been paid to this peptide and there is no method enabling its determination in soybeans. The aim of this work was the development of an analytical methodology for this purpose. A methodology consisting of the extraction of soybean proteins, their digestion with Protease P enzyme, their chromatographic separation using capillary-HPLC, and IT-MS detection was optimized. Protein extraction was performed by the use of high intensity focused ultrasounds to obtain a reduced extraction time. Optimization of chromatographic and mass spectrometry parameters enabled the separation of VLIVP peptide within just 7min and its sensitive detection. The analytical characteristics of the capillary-HPLC-IT-MS method were evaluated through the study of linearity, LOD, LOQ, study of the presence of matrix interferences, precision, and recovery. The method enabled to detect as low as 3.6ng of peptide and to determine as low as 12ng of peptide in 1g of soybean (as dry basis). Finally, the developed method was applied to the determination of the antihypertensive peptide VLIVP in different soybean varieties. The results showed the highest yield of VLIVP peptide in variety Mazowiecka II from Poland. PMID:24630980

  8. Midfrontal theta tracks action monitoring over multiple interactive time scales.

    Science.gov (United States)

    Cohen, Michael X

    2016-11-01

    Quickly detecting and correcting mistakes is a crucial brain function. EEG studies have identified an idiosyncratic electrophysiological signature of online error correction, termed midfrontal theta. Midfrontal theta has so far been investigated over the fast time-scale of a few hundred milliseconds. But several aspects of behavior and brain activity unfold over multiple time scales, displaying "scale-free" dynamics that have been linked to criticality and optimal flexibility when responding to changing environmental demands. Here we used a novel line-tracking task to demonstrate that midfrontal theta is a transient yet non-phase-locked response that is modulated by task performance over at least three time scales: a few hundred milliseconds at the onset of a mistake, task performance over a fixed window of the previous 5s, and scale-free-like fluctuations over many tens of seconds. These findings provide novel evidence for a role of midfrontal theta in online behavioral adaptation, and suggest new approaches for linking EEG signatures of human executive functioning to its neurobiological underpinnings.

  9. Midfrontal theta tracks action monitoring over multiple interactive time scales.

    Science.gov (United States)

    Cohen, Michael X

    2016-11-01

    Quickly detecting and correcting mistakes is a crucial brain function. EEG studies have identified an idiosyncratic electrophysiological signature of online error correction, termed midfrontal theta. Midfrontal theta has so far been investigated over the fast time-scale of a few hundred milliseconds. But several aspects of behavior and brain activity unfold over multiple time scales, displaying "scale-free" dynamics that have been linked to criticality and optimal flexibility when responding to changing environmental demands. Here we used a novel line-tracking task to demonstrate that midfrontal theta is a transient yet non-phase-locked response that is modulated by task performance over at least three time scales: a few hundred milliseconds at the onset of a mistake, task performance over a fixed window of the previous 5s, and scale-free-like fluctuations over many tens of seconds. These findings provide novel evidence for a role of midfrontal theta in online behavioral adaptation, and suggest new approaches for linking EEG signatures of human executive functioning to its neurobiological underpinnings. PMID:27475291

  10. Mechanism and kinetics of peptide partitioning into membranes from all-atom simulations of thermostable peptides

    OpenAIRE

    Ulmschneider, Martin B.; Doux, Jacques P F; Killian, J. Antoinette; Smith, Jeremy C.; Ulmschneider, Jakob P.

    2010-01-01

    Partitioning properties of transmembrane (TM) polypeptide segments directly determine membrane protein folding, stability, and function, and their understanding is vital for rational design of membrane active peptides. However, direct determination of water-to-bilayer transfer of TM peptides has proved difficult. Experimentally, sufficiently hydrophobic peptides tend to aggregate, while atomistic computer simulations at physiological temperatures cannot yet reach the long time scales required...

  11. Quantification of glycated N-terminal peptide of hemoglobin using derivatization for multiple functional groups of amino acids followed by liquid chromatography/tandem mass spectrometry.

    Science.gov (United States)

    Sakaguchi, Yohei; Kinumi, Tomoya; Yamazaki, Taichi; Takatsu, Akiko

    2016-02-01

    A novel method of amino acid analysis using derivatization of multiple functional groups (amino, carboxyl, and phenolic hydroxyl groups) was applied to measure glycated amino acids in order to quantify glycated peptides and evaluate the degree of glycation of peptide. Amino and carboxyl groups of amino acids were derivatized with 1-bromobutane so that the hydrophobicities and basicities of the amino acids, including glycated amino acids, were improved. These derivatized amino acids could be detected with high sensitivity using LC-MS/MS. In this study, 1-deoxyfructosyl-VHLTPE and VHLTPE, which are N-terminal peptides of the β-chains of hemoglobin, were selected as target compounds. After reducing the peptide sample solution with sodium borohydride, the obtained peptides were hydrolyzed with hydrochloric acid. The released amino acids were then derivatized with 1-bromobutane and analyzed with LC-MS/MS. The derivatized amino acids, including glycated amino acids, could be separated using an octadecyl silylated silica column and good sharp peaks were detected. We show a confirmatory experiment that the proposed method can be applied to evaluate the degree of glycation of peptides, using mixtures of glycated and non-glycated peptide.

  12. Identification and formation of angiotensin-converting enzyme-inhibitory peptides in Manchego cheese by high-performance liquid chromatography-tandem mass spectrometry.

    Science.gov (United States)

    Gómez-Ruiz, José Angel; Ramos, Mercedes; Recio, Isidra

    2004-10-29

    A total of 75 peptides included in the fraction with molecular mass below 3000 from an 8-month-old Manchego cheese could be identified using HPLC coupled on line to an ion trap mass spectrometer. Some previously described peptides with antihypertensive and/or angiotensin-converting enzyme (ACE)-inhibitory activity were detected. The formation of five active sequences was followed during cheese ripening in four different batches of Manchego cheese. Two experimental batches of Manchego cheese elaborated with selected bacterial strains with the aim of improve the organoleptic characteristics demonstrated also a good performance in the formation of peptides with ACE-inhibitory activity during cheese ripening. PMID:15553153

  13. The Role of Time-Scales in Socio-hydrology

    Science.gov (United States)

    Blöschl, Günter; Sivapalan, Murugesu

    2016-04-01

    Much of the interest in hydrological modeling in the past decades revolved around resolving spatial variability. With the rapid changes brought about by human impacts on the hydrologic cycle, there is now an increasing need to refocus on time dependency. We present a co-evolutionary view of hydrologic systems, in which every part of the system including human systems, co-evolve, albeit at different rates. The resulting coupled human-nature system is framed as a dynamical system, characterized by interactions of fast and slow time scales and feedbacks between environmental and social processes. This gives rise to emergent phenomena such as the levee effect, adaptation to change and system collapse due to resource depletion. Changing human values play a key role in the emergence of these phenomena and should therefore be considered as internal to the system in a dynamic way. The co-evolutionary approach differs from the traditional view of water resource systems analysis as it allows for path dependence, multiple equilibria, lock-in situations and emergent phenomena. The approach may assist strategic water management for long time scales through facilitating stakeholder participation, exploring the possibility space of alternative futures, and helping to synthesise the observed dynamics of different case studies. Future research opportunities include the study of how changes in human values are connected to human-water interactions, historical analyses of trajectories of system co-evolution in individual places and comparative analyses of contrasting human-water systems in different climate and socio-economic settings. Reference Sivapalan, M. and G. Blöschl (2015) Time scale interactions and the coevolution of humans and water. Water Resour. Res., 51, 6988-7022, doi:10.1002/2015WR017896.

  14. Tunneling time scale of under-the-barrier forerunners

    CERN Document Server

    García-Calderón, G; Garcia-Calderon, Gaston; Villavicencio, Jorge

    2002-01-01

    Time-dependent analytical solutions to Schr\\"{o}dinger's equation with quantum shutter initial conditions are used to investigate the issue of the tunneling time of forerunners in rectangular potential barriers. By using a time-frequency analysis, we find the existence of a regime characterized by the opacity of the barrier, where the maximum peak of a forerunner in time domain corresponds to a genuine tunneling process. The corresponding time scale represents the tunneling time of the forerunner through the classically forbidden region.

  15. Anomalous multiphoton photoelectric effect in ultrashort time scales.

    Science.gov (United States)

    Kupersztych, J; Raynaud, M

    2005-09-30

    In a multiphoton photoelectric process, an electron needs to absorb a given number of photons to escape the surface of a metal. It is shown for the first time that this number is not a constant depending only on the characteristics of the metal and light, but varies with the interaction duration in ultrashort time scales. The phenomenon occurs when electromagnetic energy is transferred, via ultrafast excitation of electron collective modes, to conduction electrons in a duration less than the electron energy damping time. It manifests itself through a dramatic increase of electron production.

  16. Separation of Time Scales in a Quantum Newton's Cradle

    Science.gov (United States)

    van den Berg, R.; Wouters, B.; Eliëns, S.; De Nardis, J.; Konik, R. M.; Caux, J.-S.

    2016-06-01

    We provide detailed modeling of the Bragg pulse used in quantum Newton's-cradle-like settings or in Bragg spectroscopy experiments for strongly repulsive bosons in one dimension. We reconstruct the postpulse time evolution and study the time-dependent local density profile and momentum distribution by a combination of exact techniques. We further provide a variety of results for finite interaction strengths using a time-dependent Hartree-Fock analysis and bosonization-refermionization techniques. Our results display a clear separation of time scales between rapid and trap-insensitive relaxation immediately after the pulse, followed by slow in-trap periodic behavior.

  17. The MOND limit from space-time scale invariance

    OpenAIRE

    Milgrom, Mordehai

    2008-01-01

    The MOND limit is shown to follow from a requirement of space-time scale invariance of the equations of motion for nonrelativistic, purely gravitational systems; i.e., invariance of the equations of motion under (t,r) goes to (qt,qr), in the limit a0 goes to infinity. It is suggested that this should replace the definition of the MOND limit based on the low-acceleration behavior of a Newtonian-MOND interpolating function. In this way, the salient, deep-MOND results--asymptotically flat rotati...

  18. Brownian motion at fast time scales and thermal noise imaging

    Science.gov (United States)

    Huang, Rongxin

    This dissertation presents experimental studies on Brownian motion at fast time scales, as well as our recent developments in Thermal Noise Imaging which uses thermal motions of microscopic particles for spatial imaging. As thermal motions become increasingly important in the studies of soft condensed matters, the study of Brownian motion is not only of fundamental scientific interest but also has practical applications. Optical tweezers with a fast position-sensitive detector provide high spatial and temporal resolution to study Brownian motion at fast time scales. A novel high bandwidth detector was developed with a temporal resolution of 30 ns and a spatial resolution of 1 A. With this high bandwidth detector, Brownian motion of a single particle confined in an optical trap was observed at the time scale of the ballistic regime. The hydrodynamic memory effect was fully studied with polystyrene particles of different sizes. We found that the mean square displacements of different sized polystyrene particles collapse into one master curve which is determined by the characteristic time scale of the fluid inertia effect. The particle's inertia effect was shown for particles of the same size but different densities. For the first time the velocity autocorrelation function for a single particle was shown. We found excellent agreement between our experiments and the hydrodynamic theories that take into account the fluid inertia effect. Brownian motion of a colloidal particle can be used to probe three-dimensional nano structures. This so-called thermal noise imaging (TNI) has been very successful in imaging polymer networks with a resolution of 10 nm. However, TNI is not efficient at micrometer scale scanning since a great portion of image acquisition time is wasted on large vacant volume within polymer networks. Therefore, we invented a method to improve the efficiency of large scale scanning by combining traditional point-to-point scanning to explore large vacant

  19. Units of relativistic time scales and associated quantities

    OpenAIRE

    Klioner, S.; Capitaine, N.; Folkner, W.; Guinot, B.; Huang, T.-Y.; Kopeikin, S.; Pitjeva, E.; Seidelmann, P. K.; Soffel, M.

    2009-01-01

    This note suggests nomenclature for dealing with the units of various astronomical quantities that are used with the relativistic time scales TT, TDB, TCB and TCG. It is suggested to avoid wordings like "TDB units" and "TT units" and avoid contrasting them to "SI units". The quantities intended for use with TCG, TCB, TT or TDB should be called "TCG-compatible", "TCB-compatible", "TT-compatible" or "TDB-compatible", respectively. The names of the units second and meter for numerical values of ...

  20. Units of relativistic time scales and associated quantities

    CERN Document Server

    Klioner, S; Folkner, W; Guinot, B; Huang, T -Y; Kopeikin, S; Pitjeva, E; Seidelmann, P K; Soffel, M

    2009-01-01

    This note suggests nomenclature for dealing with the units of various astronomical quantities that are used with the relativistic time scales TT, TDB, TCB and TCG. It is suggested to avoid wordings like "TDB units" and "TT units" and avoid contrasting them to "SI units". The quantities intended for use with TCG, TCB, TT or TDB should be called "TCG-compatible", "TCB-compatible", "TT-compatible" or "TDB-compatible", respectively. The names of the units second and meter for numerical values of all these quantities should be used with out any adjectives. This suggestion comes from a special discussion forum created within IAU Commission 52 "Relativity in Fundamental Astronomy".

  1. A Convergent Online Single Time Scale Actor Critic Algorithm

    CERN Document Server

    Di Castro, D

    2009-01-01

    Actor-Critic based approaches were among the first to address reinforcement learning in a general setting. Recently, these algorithms have gained renewed interest due to their generality, good convergence properties, and possible biological relevance. In this paper, we introduce an online temporal difference based actor-critic algorithm which is proved to converge to a neighborhood of a local maximum of the average reward. Linear function approximation is used by the critic in order estimate the value function, and the temporal difference signal, which is passed from the critic to the actor. The main distinguishing feature of the present convergence proof is that both the actor and the critic operate on a similar time scale, while in most current convergence proofs they are required to have very different time scales in order to converge. Moreover, the same temporal difference signal is used to update the parameters of both the actor and the critic. A limitation of the proposed approach, compared to results a...

  2. Empirical mode decomposition using variable filtering with time scale calibrating

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    A novel and efficient method for decomposing a signal into a set of intrinsic mode functions (IMFs) and a trend is proposed. Unlike the original empirical mode decomposition (EMD), which uses spline fits to extract variations from the signal by separating the local mean from the fluctuations in the decomposing process, this new method being proposed takes advantage of the theory of variable finite impulse response (FIR) filtering where filter coefficients and breakpoint frequencies can be adjusted to track any peak-to-peak time scale changes. The IMFs are results of a multiple variable frequency response FIR filtering when signals pass through the filters. Numerical examples validate that in contrast with the original EMD, the proposed method can fine-tune the frequency resolution and suppress the aliasing effectively.

  3. Variation of atmospheric depth profile on different time scales

    CERN Document Server

    Wilczynska, B; Homola, P; Pekala, J; Risse, M; Wilczynski, H

    2006-01-01

    The vertical profile of atmospheric depth is an important element in extensive air shower studies. The depth of shower maximum is one of the most important characteristics of the shower. In the fluorescence technique of shower detection, the geometrical reconstruction provides the altitude of shower maximum, so that an accurate profile of atmospheric depth is needed to convert this altitude to the depth of shower maximum. In this paper the temporal variation of experimentally measured profiles of atmospheric depth at different sites is studied and implications for shower reconstruction are shown. The atmospheric profiles vary on time scales from hours to years. It is shown that the daily variation of the profile is as important as its seasonal variation and should be accounted for in air shower studies. For precise shower reconstruction, the daily profiles determined locally at the site of the air shower detector are recommended.

  4. A Review of Time-Scale Modification of Music Signals

    Directory of Open Access Journals (Sweden)

    Jonathan Driedger

    2016-02-01

    Full Text Available Time-scale modification (TSM is the task of speeding up or slowing down an audio signal’s playback speed without changing its pitch. In digital music production, TSM has become an indispensable tool, which is nowadays integrated in a wide range of music production software. Music signals are diverse—they comprise harmonic, percussive, and transient components, among others. Because of this wide range of acoustic and musical characteristics, there is no single TSM method that can cope with all kinds of audio signals equally well. Our main objective is to foster a better understanding of the capabilities and limitations of TSM procedures. To this end, we review fundamental TSM methods, discuss typical challenges, and indicate potential solutions that combine different strategies. In particular, we discuss a fusion approach that involves recent techniques for harmonic-percussive separation along with time-domain and frequency-domain TSM procedures.

  5. Nonoscillation for second order sublinear dynamic equations on time scales

    Science.gov (United States)

    Erbe, Lynn; Baoguo, Jia; Peterson, Allan

    2009-10-01

    Consider the Emden-Fowler sublinear dynamic equation x[Delta][Delta](t)+p(t)f(x([sigma](t)))=0, where , is a time scale, , where ai>0, 0researchers. In this paper, we allow the coefficient function p(t) to be negative for arbitrarily large values of t. We extend a nonoscillation result of Wong for the second order sublinear Emden-Fowler equation in the continuous case to the dynamic equation (0.1). As applications, we show that the sublinear difference equation has a nonoscillatory solution, for b>0, c>[alpha], and the sublinear q-difference equation has a nonoscillatory solution, for , q>1, b>0, c>1+[alpha].

  6. Estimating ventilation time scales using overturning stream functions

    Science.gov (United States)

    Thompson, Bijoy; Nycander, Jonas; Nilsson, Johan; Jakobsson, Martin; Döös, Kristofer

    2014-06-01

    A simple method for estimating ventilation time scales from overturning stream functions is proposed. The stream function may be computed using either geometric coordinates or a generalized vertical coordinate, such as potential density (salinity in our study). The method is tested with a three-dimensional circulation model describing an idealized semi-enclosed ocean basin ventilated through a narrow strait over a sill, and the result is compared to age estimates obtained from a passive numerical age tracer. The best result is obtained when using the stream function in salinity coordinates. In this case, the reservoir-averaged advection time obtained from the overturning stream function in salinity coordinates agrees rather well with the mean age of the age tracer, and the corresponding maximum ages agree very well.

  7. On the superposition of heterogeneous traffic at large time scales

    Directory of Open Access Journals (Sweden)

    Sidney I. Resnick

    2011-01-01

    Full Text Available Various empirical and theoretical studies indicate that cumulative network traffic is a Gaussian process. However, depending on whether the intensity at which sessions are initiated is large or small relative to the session duration tail, [25] and [15] have shown that traffic at large time scales can be approximated by either fractional Brownian motion (fBm or stable Lévy motion. We study distributional properties of cumulative traffic that consists of a finite number of independent streams and give an explanation of why Gaussian examples abound in practice but not stable Lévy motion. We offer an explanation of how much vertical aggregation is needed for the Gaussian approximation to hold. Our results are expressed as limit theorems for a sequence of cumulative traffic processes whose session initiation intensities satisfy growth rates similar to those used in [25].

  8. Stability theory for dynamic equations on time scales

    CERN Document Server

    Martynyuk, Anatoly A

    2016-01-01

    This monograph is a first in the world to present three approaches for stability analysis of solutions of dynamic equations. The first approach is based on the application of dynamic integral inequalities and the fundamental matrix of solutions of linear approximation of dynamic equations. The second is based on the generalization of the direct Lyapunovs method for equations on time scales, using scalar, vector and matrix-valued auxiliary functions. The third approach is the application of auxiliary functions (scalar, vector, or matrix-valued ones) in combination with differential dynamic inequalities. This is an alternative comparison method, developed for time continuous and time discrete systems. In recent decades, automatic control theory in the study of air- and spacecraft dynamics and in other areas of modern applied mathematics has encountered problems in the analysis of the behavior of solutions of time continuous-discrete linear and/or nonlinear equations of perturbed motion. In the book “Men of Ma...

  9. Two-time-scale population evolution on a singular landscape

    Science.gov (United States)

    Xu, Song; Jiao, Shuyun; Jiang, Pengyao; Ao, Ping

    2014-01-01

    Under the effect of strong genetic drift, it is highly probable to observe gene fixation or gene loss in a population, shown by singular peaks on a potential landscape. The genetic drift-induced noise gives rise to two-time-scale diffusion dynamics on the bipeaked landscape. We find that the logarithmically divergent (singular) peaks do not necessarily imply infinite escape times or biological fixations by iterating the Wright-Fisher model and approximating the average escape time. Our analytical results under weak mutation and weak selection extend Kramers's escape time formula to models with B (Beta) function-like equilibrium distributions and overcome constraints in previous methods. The constructed landscape provides a coherent description for the bistable system, supports the quantitative analysis of bipeaked dynamics, and generates mathematical insights for understanding the boundary behaviors of the diffusion model.

  10. Units of relativistic time scales and associated quantities

    Science.gov (United States)

    Klioner, S. A.; Capitaine, N.; Folkner, W. M.; Guinot, B.; Huang, T.-Y.; Kopeikin, S. M.; Pitjeva, E. V.; Seidelmann, P. K.; Soffel, M. H.

    2010-01-01

    This note suggests nomenclature for dealing with the units of various astronomical quantities that are used with the relativistic time scales TT, TDB, TCB and TCG. It is suggested to avoid wordings like “TDB units” and “TT units” and avoid contrasting them to “SI units”. The quantities intended for use with TCG, TCB, TT or TDB should be called “TCG-compatible”, “TCB-compatible”, “TT-compatible” or “TDB-compatible”, respectively. The names of the units second and meter for numerical values of all these quantities should be used without any adjectives. This suggestion comes from a special discussion forum created within IAU Commission 52 “Relativity in Fundamental Astronomy”.

  11. Shock response of iron on nanosecond time scales

    International Nuclear Information System (INIS)

    Experiments were performed using direct laser irradiation to induce shock waves in pure Fe foils tens of microns thick, using surface velocimetry to measure the response. Compared with experiments on much thicker samples, relatively strong elastic precursors were observed, though these could be caused by the strong texture of the foils rather than the shorter time scale. There was also evidence for the onset of the bcc to hcp phase transition, starting close to the static phase boundary. Radiation hydrodynamics and continuum mechanical simulations were performed to interpret the velocity data, comparing several equations of state with polymorphism and strength. The data were consistent with a phase change rate of the order of 109/s, driven by superpressurization of about 5 GPa. The rate may also depend on texture

  12. 色谱纯化和质谱分析法研究牛骨源咸味肽%Separation, Purification and Analysis of Salty Peptides Derived from Bovine Bone by Chromatography and Mass Spectrometry

    Institute of Scientific and Technical Information of China (English)

    李迎楠; 刘文营; 张顺亮; 成晓瑜

    2016-01-01

    以牛骨为研究对象,通过中空纤维超滤装置、Sephadex G-25凝胶色谱柱对相对分子质量小于5000的酶解产物进行初步分离,选用制备型及分析型高效液相色谱分析仪对咸味肽进行纯化、收集及分析。结果表明:收集得到牛骨源咸味肽为单一组分;用基质辅助激光解析电离-飞行时间质谱仪对咸味肽进行分析,得到其为相对分子质量均小于1000的短肽,其呈现咸味的物质质荷比值可能为679.5109。%The enzymatic hydrolysate of bovine bone with relative molecular weight < 5 000 was separated by hollow fiber ultrafiltration and Sephadex G-25 column chromatography. Two salty peptide fractions from the hydrolysate were purified by preparative high performance liquid chromatography (HPLC) and the pooled samples were analyzed by HPLC. The two fractions were found to consist of a single homogenous component. As analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS), both peptide fractions had a relative molecular weight less than 1000, the salty taste compound of which was m/z 679.510 9.

  13. High-performance liquid chromatography matrix-assisted laser desorption/ionization time-of-flight mass spectrometry peptide fingerprinting of tarantula venoms in the genus Brachypelma: chemotaxonomic and biochemical applications.

    Science.gov (United States)

    Escoubas, P; Célérier, M L; Nakajima, T

    1997-01-01

    Precise identification of arthropod species is fundamental in venom research, particularly in groups where taxonomy problems remain unsolved. High-performance liquid chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) analysis of crude venoms of six tarantula species in the genus Brachypelma showed that the characteristic chromatographic and peptide ion profiles obtained can be used to discriminate amongst closely related species. This method permits rapid mass fingerprinting of large numbers of samples in a reproducible manner, and offers a powerful systematic tool in combination with morphological methods for the classification of tarantula species. The sensitivity and precision of the method may offer a way to solve complex taxonomic relationships not easily resolved by morphological measurements, in a non-destructive manner. Additionally, peptide mapping of crude venoms by MALDI-TOFMS will speed up the discovery of novel ligands of neuronal receptors, since major venom components of related species share a high sequence homology and are likely to possess similar pharmacological properties. PMID:9404038

  14. Neural Computations in a Dynamical System with Multiple Time Scales.

    Science.gov (United States)

    Mi, Yuanyuan; Lin, Xiaohan; Wu, Si

    2016-01-01

    Neural systems display rich short-term dynamics at various levels, e.g., spike-frequency adaptation (SFA) at the single-neuron level, and short-term facilitation (STF) and depression (STD) at the synapse level. These dynamical features typically cover a broad range of time scales and exhibit large diversity in different brain regions. It remains unclear what is the computational benefit for the brain to have such variability in short-term dynamics. In this study, we propose that the brain can exploit such dynamical features to implement multiple seemingly contradictory computations in a single neural circuit. To demonstrate this idea, we use continuous attractor neural network (CANN) as a working model and include STF, SFA and STD with increasing time constants in its dynamics. Three computational tasks are considered, which are persistent activity, adaptation, and anticipative tracking. These tasks require conflicting neural mechanisms, and hence cannot be implemented by a single dynamical feature or any combination with similar time constants. However, with properly coordinated STF, SFA and STD, we show that the network is able to implement the three computational tasks concurrently. We hope this study will shed light on the understanding of how the brain orchestrates its rich dynamics at various levels to realize diverse cognitive functions. PMID:27679569

  15. Forecasting decadal and shorter time-scale solar cycle features

    Science.gov (United States)

    Dikpati, Mausumi

    2016-07-01

    Solar energetic particles and magnetic fields reach the Earth through the interplanetary medium and affect it in various ways, producing beautiful aurorae, but also electrical blackouts and damage to our technology-dependent economy. The root of energetic solar outputs is the solar activity cycle, which is most likely caused by dynamo processes inside the Sun. It is a formidable task to accurately predict the amplitude, onset and peak timings of a solar cycle. After reviewing all solar cycle prediction methods, including empirical as well as physical model-based schemes, I will describe what we have learned from both validation and nonvalidation of cycle 24 forecasts, and how to refine the model-based schemes for upcoming cycle 25 forecasts. Recent observations indicate that within a solar cycle there are shorter time-scale 'space weather' features, such as bursts of various forms of activity with approximately one year periodicity. I will demonstrate how global tachocline dynamics could play a crucial role in producing such space weather. The National Center for Atmospheric Research is sponsored by the National Science Foundation.

  16. On the superimposition of heterogeneous traffic at large time scales

    CERN Document Server

    Lopez-Oliveros, Luis

    2010-01-01

    Various empirical and theoretical studies indicate that cumulative network traffic is a Gaussian process. However, depending on whether the intensity at which sessions are initiated is large or small relative to the session duration tail, Mikosch et a. (Ann Appl Probab, 12:23-68, 2002) and Kaj and Taqqu (Progress Probab, 60:383-427, 2008) have shown that traffic at large time scales can be approximated by either fractional Brownian motion (fBm) or stable Levy motion. We study distributional properties of cumulative traffic that consists of a finite number of independent streams and give an explanation of why Gaussian examples abound in practice but not stable Levy motion. We offer an explanation of how much vertical aggregation is needed for the Gaussian approximation to hold. Our results are expressed as limit theorems for a sequence of cumulative traffic processes whose session initiation intensities satisfy growth rates similar to those used in Mikosch et a. (Ann Appl Probab, 12:23-68, 2002).

  17. Role of relaxation time scale in noisy signal transduction.

    Science.gov (United States)

    Maity, Alok Kumar; Chaudhury, Pinaki; Banik, Suman K

    2015-01-01

    Intra-cellular fluctuations, mainly triggered by gene expression, are an inevitable phenomenon observed in living cells. It influences generation of phenotypic diversity in genetically identical cells. Such variation of cellular components is beneficial in some contexts but detrimental in others. To quantify the fluctuations in a gene product, we undertake an analytical scheme for studying few naturally abundant linear as well as branched chain network motifs. We solve the Langevin equations associated with each motif under the purview of linear noise approximation and derive the expressions for Fano factor and mutual information in close analytical form. Both quantifiable expressions exclusively depend on the relaxation time (decay rate constant) and steady state population of the network components. We investigate the effect of relaxation time constraints on Fano factor and mutual information to indentify a time scale domain where a network can recognize the fluctuations associated with the input signal more reliably. We also show how input population affects both quantities. We extend our calculation to long chain linear motif and show that with increasing chain length, the Fano factor value increases but the mutual information processing capability decreases. In this type of motif, the intermediate components act as a noise filter that tune up input fluctuations and maintain optimum fluctuations in the output. For branched chain motifs, both quantities vary within a large scale due to their network architecture and facilitate survival of living system in diverse environmental conditions. PMID:25955500

  18. Role of relaxation time scale in noisy signal transduction.

    Directory of Open Access Journals (Sweden)

    Alok Kumar Maity

    Full Text Available Intra-cellular fluctuations, mainly triggered by gene expression, are an inevitable phenomenon observed in living cells. It influences generation of phenotypic diversity in genetically identical cells. Such variation of cellular components is beneficial in some contexts but detrimental in others. To quantify the fluctuations in a gene product, we undertake an analytical scheme for studying few naturally abundant linear as well as branched chain network motifs. We solve the Langevin equations associated with each motif under the purview of linear noise approximation and derive the expressions for Fano factor and mutual information in close analytical form. Both quantifiable expressions exclusively depend on the relaxation time (decay rate constant and steady state population of the network components. We investigate the effect of relaxation time constraints on Fano factor and mutual information to indentify a time scale domain where a network can recognize the fluctuations associated with the input signal more reliably. We also show how input population affects both quantities. We extend our calculation to long chain linear motif and show that with increasing chain length, the Fano factor value increases but the mutual information processing capability decreases. In this type of motif, the intermediate components act as a noise filter that tune up input fluctuations and maintain optimum fluctuations in the output. For branched chain motifs, both quantities vary within a large scale due to their network architecture and facilitate survival of living system in diverse environmental conditions.

  19. The quenching time scale and quenching rate of galaxies

    CERN Document Server

    Lian, Jianhui; Zhang, Kai; Kong, Xu

    2016-01-01

    The average star formation rate (SFR) in galaxies has been declining since redshift of 2. A fraction of galaxies quench and become quiescent. We constrain two key properties of the quenching process: the quenching time scale and the quenching rate among galaxies. We achieve this by analyzing the galaxy number density profile in NUV-u color space and the distribution in NUV-u v.s. u-i color-color diagram with a simple toy-model framework. We focus on galaxies in three mass bins between 10 to 10 and 10 to 10.6 solar mass. In the NUV-u v.s. u-i color-color diagram, the red u-i galaxies exhibit a different slope from the slope traced by the star-forming galaxies. This angled distribution and the number density profile of galaxies in NUV-u space strongly suggest that the decline of the SFR in galaxies has to accelerate before they turn quiescent. We model this color-color distribution with a two-phase exponential decline star formation history. The models with an e-folding time in the second phase (the quenching p...

  20. Radionuclide time-scales and recent environmental changes

    International Nuclear Information System (INIS)

    North America and European lakes have been used to monitor environmental changes such as land-use activities, acidification and chemical pollution specially heavy metals, halogenated organic substances and radioactive waste. In such studies radioactive dating using various combinations of low-level counting and spectrometry allows reconstruction of high resolution chronologies of recent sediments. Modelling recent environmental changes due to natural processes and man-made activities on regional and global scales and related consequences on the quality of aquatic life could be followed through systematic studies of lakes from different geographic and climatic regions. Isotope dilution alpha spectrometry using PIPS detectors, radon emanation using ionization chambers and sensitive Ge-gamma spectrometers are utilized to measure Pb-210,Ra-226, Cs-137,Cs-134, K-40 as well as other radionuclides. The mentioned nuclides are used to determine accumulation rates in protected lakes and to evaluate erosion from soils in connection with land-use activities. In such studies Pb-210 and Cs-137 fluxes give additional information on atmospheric and non-atmospheric inputs to lakes. The constructed time-scales (0 - 150 Yrs) allowed us to monitor the evolution of anthropogenic pollution and related loads in various regions. 1 tab.; 12 refs. (author)

  1. Time scale of diffusion in molecular and cellular biology

    Science.gov (United States)

    Holcman, D.; Schuss, Z.

    2014-05-01

    Diffusion is the driver of critical biological processes in cellular and molecular biology. The diverse temporal scales of cellular function are determined by vastly diverse spatial scales in most biophysical processes. The latter are due, among others, to small binding sites inside or on the cell membrane or to narrow passages between large cellular compartments. The great disparity in scales is at the root of the difficulty in quantifying cell function from molecular dynamics and from simulations. The coarse-grained time scale of cellular function is determined from molecular diffusion by the mean first passage time of molecular Brownian motion to a small targets or through narrow passages. The narrow escape theory (NET) concerns this issue. The NET is ubiquitous in molecular and cellular biology and is manifested, among others, in chemical reactions, in the calculation of the effective diffusion coefficient of receptors diffusing on a neuronal cell membrane strewn with obstacles, in the quantification of the early steps of viral trafficking, in the regulation of diffusion between the mother and daughter cells during cell division, and many other cases. Brownian trajectories can represent the motion of a molecule, a protein, an ion in solution, a receptor in a cell or on its membrane, and many other biochemical processes. The small target can represent a binding site or an ionic channel, a hidden active site embedded in a complex protein structure, a receptor for a neurotransmitter on the membrane of a neuron, and so on. The mean time to attach to a receptor or activator determines diffusion fluxes that are key regulators of cell function. This review describes physical models of various subcellular microdomains, in which the NET coarse-grains the molecular scale to a higher cellular-level, thus clarifying the role of cell geometry in determining subcellular function.

  2. Monolithic porous polymer stationary phases in polyimide chips for the fast high-performance liquid chromatography separation of proteins and peptides

    OpenAIRE

    Levkin, Pavel A.; Eeltink, Sebastiaan; Stratton, Thomas R.; Brennen, Reid; Robotti, Karla; Yin, Hongfeng; Killeen, Kevin; Svec, Frantisek; Fréchet, Jean M.J.

    2008-01-01

    Poly(lauryl methacrylate-co-ethylene dimethacrylate) and poly(styrene-co-divinylbenzene) stationary phases in monolithic format have been prepared by thermally initiated free radical polymerization within polyimide chips featuring channels having a cross-section of 200×200 μm and a length of 6.8 cm. These chips were then used for the separation of a mixture of proteins including ribonuclease A, myoglobin, cytochrome c, and ovalbumin, as well as peptides. The separations were monitored by UV a...

  3. Advances in the Geomagnetic Polarity Time Scale--Developments and Integration with the Geologic Time Scale and Future Directions (Invited)

    Science.gov (United States)

    Geissman, J. W.

    2013-12-01

    We celebrate the 50th anniversary of the publication of the Vine-Matthews/Morley-Larochelle hypothesis (Vine and Matthews, Nature, 1963, v. 199, #4897, p. 947-949), which integrated marine magnetic anomaly data with a rapidly evolving terrestrial-based geomagnetic polarity time scale (GPTS). The five decades of research since 1963 have witnessed the expansion and refinement of the GPTS, to the point where ages of magnetochron boundaries, in particular in the Cenozoic, can be estimated with uncertainties better than 0.1%. This has come about by integrating high precision geochronology, cyclostratigraphy at different time scales, and magnetic polarity data of increased quality, allowing extension of the GPTS back into the Paleozoic. The definition of a high resolution GPTS across time intervals of major events in Earth history has been of particular interest, as a specific magnetochron boundary correlated across several localities represents a singular global datum. A prime example is the end Permian, when some 80 percent of genus-level extinctions and a range of 75 to 96 percent species- level extinctions took place in the marine environment, depending upon clade. Much our understanding of the Permian-Triassic boundary (PTB) is based on relatively slowly deposited marine sequences in Europe and Asia, yet a growing body of observations from continental sequences demonstrates a similar extinction event and new polarity data from some of these sequences are critical to refining the GPTS across the PTB and testing synchronicity of marine and terrestrial events. The data show that the end-Permian ecological crisis and the conodont calibrated biostratigraphic PTB both followed a key polarity reversal between a short interval (subchron) of reverse polarity to a considerably longer (chron) of normal polarity. Central European Basin strata (continental Permian and epicontinental Triassic) yield high-quality magnetic polarity stratigraphic records (Szurlies et al., 2003

  4. Two-phase micro- and macro-time scales in particle-laden turbulent channel flows

    Institute of Scientific and Technical Information of China (English)

    Bing Wang; Michael Manhart

    2012-01-01

    The micro- and macro-time scales in two-phase turbulent channel flows are investigated using the direct numerical simulation and the Lagrangian particle trajectory methods for the fluid- and the particle-phases,respectively.Lagrangian and Eulerian time scales of both phases are calculated using velocity correlation functions.Due to flow anisotropy,micro-time scales are not the same with the theoretical estimations in large Reynolds number (isotropic) turbulence.Lagrangian macro-time scales of particle-phase and of fluid-phase seen by particles are both dependent on particle Stokes number.The fluid-phase Lagrangian integral time scales increase with distance from the wall,longer than those time scales seen by particles.The Eulerian integral macro-time scales increase in near-wall regions but decrease in out-layer regions.The moving Eulerian time scales are also investigated and compared with Lagrangian integral time scales,and in good agreement with previous measurements and numerical predictions.For the fluid particles the micro Eulerian time scales are longer than the Lagrangian ones in the near wall regions,while away from the walls the micro Lagrangian time scales are longer.The Lagrangian integral time scales are longer than the Eulerian ones.The results are useful for further understanding two-phase flow physics and especially for constructing accurate prediction models of inertial particle dispersion.

  5. Multiple time scales and the lifetime coefficient of variation: engineering applications.

    Science.gov (United States)

    Kordonsky, K B; Gertsbakh, I

    1997-01-01

    We consider linear combinations of "natural" time scales and choose the "best" one which provides the minimum coefficient of variation of the lifetime. Our time scale is in fact a generalized Miner time scale because the latter is based on an appropriate weighting of the times spent on low and high level loadings. The suggested modus operandi for finding the "best" time scale has many features in common with the approach suggested by Farewell and Cox (1979) and Oakes (1995) which is devoted to multiple time scales in survival analysis.

  6. Input-output description of linear systems with multiple time-scales

    Science.gov (United States)

    Madriz, R. S.; Sastry, S. S.

    1984-01-01

    It is pointed out that the study of systems evolving at multiple time-scales is simplified by studying reduced-order models of these systems valid at specific time-scales. The present investigation is concerned with an extension of results on the time-scale decomposition of autonomous systems to that of input-output systems. The results are employed to study conditions under which positive realness of a transfer function is preserved under singular perturbation. Attention is given to the perturbation theory for linear operators, the multiple time-scale structure of autonomous linear systems, the input-output description of two time-scale linear systems, the positive realness of two time-scale systems, and multiple time-scale linear systems.

  7. Simple setup for gas-phase H/D exchange mass spectrometry coupled to electron transfer dissociation and ion mobility for analysis of polypeptide structure on a liquid chromatographic time scale.

    Science.gov (United States)

    Mistarz, Ulrik H; Brown, Jeffery M; Haselmann, Kim F; Rand, Kasper D

    2014-12-01

    Gas-phase hydrogen/deuterium exchange (HDX) is a fast and sensitive, yet unharnessed analytical approach for providing information on the structural properties of biomolecules, in a complementary manner to mass analysis. Here, we describe a simple setup for ND3-mediated millisecond gas-phase HDX inside a mass spectrometer immediately after ESI (gas-phase HDX-MS) and show utility for studying the primary and higher-order structure of peptides and proteins. HDX was achieved by passing N2-gas through a container filled with aqueous deuterated ammonia reagent (ND3/D2O) and admitting the saturated gas immediately upstream or downstream of the primary skimmer cone. The approach was implemented on three commercially available mass spectrometers and required no or minor fully reversible reconfiguration of gas-inlets of the ion source. Results from gas-phase HDX-MS of peptides using the aqueous ND3/D2O as HDX reagent indicate that labeling is facilitated exclusively through gaseous ND3, yielding similar results to the infusion of purified ND3-gas, while circumventing the complications associated with the use of hazardous purified gases. Comparison of the solution-phase- and gas-phase deuterium uptake of Leu-Enkephalin and Glu-Fibrinopeptide B, confirmed that this gas-phase HDX-MS approach allows for labeling of sites (heteroatom-bound non-amide hydrogens located on side-chains, N-terminus and C-terminus) not accessed by classical solution-phase HDX-MS. The simple setup is compatible with liquid chromatography and a chip-based automated nanoESI interface, allowing for online gas-phase HDX-MS analysis of peptides and proteins separated on a liquid chromatographic time scale at increased throughput. Furthermore, online gas-phase HDX-MS could be performed in tandem with ion mobility separation or electron transfer dissociation, thus enabling multiple orthogonal analyses of the structural properties of peptides and proteins in a single automated LC-MS workflow.

  8. Simple setup for gas-phase H/D exchange mass spectrometry coupled to electron transfer dissociation and ion mobility for analysis of polypeptide structure on a liquid chromatographic time scale.

    Science.gov (United States)

    Mistarz, Ulrik H; Brown, Jeffery M; Haselmann, Kim F; Rand, Kasper D

    2014-12-01

    Gas-phase hydrogen/deuterium exchange (HDX) is a fast and sensitive, yet unharnessed analytical approach for providing information on the structural properties of biomolecules, in a complementary manner to mass analysis. Here, we describe a simple setup for ND3-mediated millisecond gas-phase HDX inside a mass spectrometer immediately after ESI (gas-phase HDX-MS) and show utility for studying the primary and higher-order structure of peptides and proteins. HDX was achieved by passing N2-gas through a container filled with aqueous deuterated ammonia reagent (ND3/D2O) and admitting the saturated gas immediately upstream or downstream of the primary skimmer cone. The approach was implemented on three commercially available mass spectrometers and required no or minor fully reversible reconfiguration of gas-inlets of the ion source. Results from gas-phase HDX-MS of peptides using the aqueous ND3/D2O as HDX reagent indicate that labeling is facilitated exclusively through gaseous ND3, yielding similar results to the infusion of purified ND3-gas, while circumventing the complications associated with the use of hazardous purified gases. Comparison of the solution-phase- and gas-phase deuterium uptake of Leu-Enkephalin and Glu-Fibrinopeptide B, confirmed that this gas-phase HDX-MS approach allows for labeling of sites (heteroatom-bound non-amide hydrogens located on side-chains, N-terminus and C-terminus) not accessed by classical solution-phase HDX-MS. The simple setup is compatible with liquid chromatography and a chip-based automated nanoESI interface, allowing for online gas-phase HDX-MS analysis of peptides and proteins separated on a liquid chromatographic time scale at increased throughput. Furthermore, online gas-phase HDX-MS could be performed in tandem with ion mobility separation or electron transfer dissociation, thus enabling multiple orthogonal analyses of the structural properties of peptides and proteins in a single automated LC-MS workflow. PMID:25375223

  9. Structure and dating errors in the geologic time scale and periodicity in mass extinctions

    Science.gov (United States)

    Stothers, Richard B.

    1989-01-01

    Structure in the geologic time scale reflects a partly paleontological origin. As a result, ages of Cenozoic and Mesozoic stage boundaries exhibit a weak 28-Myr periodicity that is similar to the strong 26-Myr periodicity detected in mass extinctions of marine life by Raup and Sepkoski. Radiometric dating errors in the geologic time scale, to which the mass extinctions are stratigraphically tied, do not necessarily lessen the likelihood of a significant periodicity in mass extinctions, but do spread the acceptable values of the period over the range 25-27 Myr for the Harland et al. time scale or 25-30 Myr for the DNAG time scale. If the Odin time scale is adopted, acceptable periods fall between 24 and 33 Myr, but are not robust against dating errors. Some indirect evidence from independently-dated flood-basalt volcanic horizons tends to favor the Odin time scale.

  10. Almost Periodic Solutions for Neutral-Type BAM Neural Networks with Delays on Time Scales

    OpenAIRE

    Yongkun Li; Li Yang

    2013-01-01

    Using the existence of the exponential dichotomy of linear dynamic equations on time scales, a fixed point theorem and the theory of calculus on time scales, we obtain some sufficient conditions for the existence and exponential stability of almost periodic solutions for a class of neutral-type BAM neural networks with delays on time scales. Finally, a numerical example illustrates the feasibility of our results and also shows that the continuous-time neural network and its dis...

  11. Hamilton-Jacobi-Bellman equations and approximate dynamic programming on time scales.

    Science.gov (United States)

    Seiffertt, John; Sanyal, Suman; Wunsch, Donald C

    2008-08-01

    The time scales calculus is a key emerging area of mathematics due to its potential use in a wide variety of multidisciplinary applications. We extend this calculus to approximate dynamic programming (ADP). The core backward induction algorithm of dynamic programming is extended from its traditional discrete case to all isolated time scales. Hamilton-Jacobi-Bellman equations, the solution of which is the fundamental problem in the field of dynamic programming, are motivated and proven on time scales. By drawing together the calculus of time scales and the applied area of stochastic control via ADP, we have connected two major fields of research. PMID:18632378

  12. Pseudospherical surfaces on time scales: a geometric definition and the spectral approach

    Energy Technology Data Exchange (ETDEWEB)

    Cieslinski, Jan L [Uniwersytet w Bialymstoku, Wydzial Fizyki, ul Lipowa 41, 15-424 Bialystok (Poland)

    2007-10-19

    We define and discuss the notion of pseudospherical surfaces in asymptotic coordinates on time scales. Thus we extend well-known notions of discrete pseudospherical surfaces and smooth pseudosperical surfaces on more exotic domains (e.g., the Cantor set). In particular, we present a new expression for the discrete Gaussian curvature which turns out to be valid for asymptotic nets on any time scale. We show that asymptotic Chebyshev nets on an arbitrary time scale have constant negative Gaussian curvature. We present also the quaternion-valued spectral problem (the Lax pair) and the Darboux-Baecklund transformation for pseudospherical surfaces (in asymptotic coordinates) on arbitrary time scales.

  13. Implementation of Time-Scale Transformation Based on Continuous Wavelet Theory

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The basic objective of time-scale transformation is to compress or expand the signal in time field while keeping the same spectral properties.This paper presents two methods to derive time-scale transformation formula based on continuous wavelet transform.For an arbitrary given square-integrable function f(t),g(t) = f(t/λ) is derived by continuous wavelet transform and its inverse transform.The result shows that time-scale transformation may be obtained through the modification of the time-scale of wavelet function filter using equivalent substitution. The paper demonstrates the result by theoretic derivations and experimental simulation.

  14. Some inequalities of Ostrowski and Grüss type for triple integrals on time scales

    Directory of Open Access Journals (Sweden)

    Nazir Ahmad Mir

    2011-12-01

    Full Text Available In this paper, we establish some inequalities of Ostrowski and Grüss type for triple integrals on arbitrary time scales involving three functions and their partial derivatives. We also discuss the discrete Ostrowski and Grüss type inequalities for triple sumon time scale.

  15. Some inequalities of Ostrowski and Grüss type for triple integrals on time scales

    OpenAIRE

    Nazir Ahmad Mir; Roman Ullah

    2011-01-01

    In this paper, we establish some inequalities of Ostrowski and Grüss type for triple integrals on arbitrary time scales involving three functions and their partial derivatives. We also discuss the discrete Ostrowski and Grüss type inequalities for triple sumon time scale.

  16. Ring intermittency near the boundary of the synchronous time scales of chaotic oscillators

    OpenAIRE

    Zhuravlev, Maxim O.; Koronovskii, Alexey A.; Moskalenko, Olga I.; Ovchinnikov, Alexey A.; Hramov, Alexander E.

    2013-01-01

    In this paper we study both experimentally and numerically the intermittent behavior taking place near the boundary of the synchronous time scales of chaotic oscillators being in the regime of time scale synchronization. We have shown that the observed type of the intermittent behavior should be classified as the ring intermittency.

  17. Ring intermittency near the boundary of the synchronous time scales of chaotic oscillators.

    Science.gov (United States)

    Zhuravlev, Maxim O; Koronovskii, Alexey A; Moskalenko, Olga I; Ovchinnikov, Alexey A; Hramov, Alexander E

    2011-02-01

    In this Brief Report we study both experimentally and numerically the intermittent behavior taking place near the boundary of the synchronous time scales of chaotic oscillators being in the regime of time scale synchronization. We have shown that the observed type of the intermittent behavior should be classified as the ring intermittency.

  18. Necessary optimality conditions for the calculus of variations on time scales

    OpenAIRE

    Ferreira, Rui A. C.; Torres, Delfim F. M.

    2007-01-01

    We study more general variational problems on time scales. Previous results are generalized by proving necessary optimality conditions for (i) variational problems involving delta derivatives of more than the first order, and (ii) problems of the calculus of variations with delta-differential side conditions (Lagrange problem of the calculus of variations on time scales).

  19. The Delta-nabla Calculus of Variations for Composition Functionals on Time Scales

    OpenAIRE

    Dryl, Monika; Torres, Delfim F. M.

    2012-01-01

    We develop the calculus of variations on time scales for a functional that is the composition of a certain scalar function with the delta and nabla integrals of a vector valued field. Euler-Lagrange equations, transversality conditions, and necessary optimality conditions for isoperimetric problems, on an arbitrary time scale, are proved. Interesting corollaries and examples are presented.

  20. Global and Local Color Time Scales to Encode Timeline Events in Ion Trajectories for Glassies

    Directory of Open Access Journals (Sweden)

    J. M. Sharif

    2015-02-01

    Full Text Available Glassy compounds lead directly to high ionic conductivity. Ionic conductivity generates ion trajectories. However, these trajectories have been represented by two-dimensional graph in order to visualize the timeline events in ion trajectories. This study addresses this problem by encoding the timeline events in ion trajectories with global and local color scales. Two time scales have been introduced namely Global Color Time Scale and Local Color Time Scale. The rainbow color has been chosen to represent global time scale meanwhile solid color has been used to generate local time scale. Based on evaluation, these techniques are successful in representing timeline events in ion trajectories for understanding the complicated heterogeneous movement of ion trajectories.

  1. Sub-daily runoff simulations with parameters inferred at the daily time scale

    Directory of Open Access Journals (Sweden)

    J. E. Reynolds

    2015-08-01

    Full Text Available Concentration times in small and medium-sized watersheds (~ 100–1000 km2 are commonly less than 24 h. Flood-forecasting models then require data at sub-daily time scales, but time-series of input and runoff data with sufficient lengths are often only available at the daily time scale, especially in developing countries. This has led to a search for time-scale relationships to infer parameter values at the time scales where they are needed from the time scales where they are available. In this study, time-scale dependencies in the HBV-light conceptual hydrological model were assessed within the generalized likelihood uncertainty estimation (GLUE approach. It was hypothesised that the existence of such dependencies is a result of the numerical method or time-stepping scheme used in the models rather than a real time-scale-data dependence. Parameter values inferred showed a clear dependence on time scale when the explicit Euler method was used for modelling at the same time steps as the time scale of the input data (1–24 h. However, the dependence almost fully disappeared when the explicit Euler method was used for modelling in 1 h time steps internally irrespectively of the time scale of the input data. In other words, it was found that when an adequate time-stepping scheme was implemented, parameter sets inferred at one time scale (e.g., daily could be used directly for runoff simulations at other time scales (e.g., 3 or 6 h without any time scaling and this approach only resulted in a small (if any model performance decrease, in terms of Nash–Sutcliffe and volume-error efficiencies. The overall results of this study indicated that as soon as sub-daily driving data can be secured, flood forecasting in watersheds with sub-daily concentration times is possible with model-parameter values inferred from long time series of daily data, as long as an appropriate numerical method is used.

  2. Environmental records of lacustrine sediments in different time scales:Sediment grain size as an example

    Institute of Scientific and Technical Information of China (English)

    CHEN Jing'an; WAN Guojiang; David Dian Zhang; ZHANG Feng; HUANG Ronggui

    2004-01-01

    Sediment grain size is a conventional proxy of climatic changes. Larger sediment grain size is often interpreted to indicate dry climate during lower lake level while smaller sediment grain size implies wet climate during higher lake level. Through detailed study on sediment grain sizes in Lake Erhai and Lake Chenghai, this paper reveals the different indication significances of sediment grain sizes in different time scales, different resolution investigations. For long time-scale and low resolution (102a or 103a) studies, larger sediment grain size indicates lower lake level, smaller lake area and drier climate while smaller sediment grain size indicates higher lake level, larger lake area and wetter climate. For short time-scale and high resolution (a or 10a) studies, larger sediment grain size reflects more rainfall and wetter climate while smaller sediment grain size reflects less rainfall and drier climate. Environmental information revealed by lacustrine sediment records is often different in different time scales because of the variance of sedimentation resolution, sampling resolution and dating precision. Therefore, paleoclimate implications of environmental proxies in long time-scale and low resolution investigations could not be mechanically applied to short time-scale and high resolution studies. Only after synthetically analyzing the influence manner and extent of all factors on the sediment records in different time scales, can credible conclusions be obtained.

  3. Environmental records of lacustrine sediments in different time scales: Sediment grain size as an example

    Institute of Scientific and Technical Information of China (English)

    CHEN; Jing'an; WAN; Guojiang; David; Dian; Zhang; ZHANG; F

    2004-01-01

    Sediment grain size is a conventional proxy of climatic changes. Larger sediment grain size is often interpreted to indicate dry climate during lower lake level while smaller sediment grain size implies wet climate during higher lake level. Through detailed study on sediment grain sizes in Lake Erhai and Lake Chenghai, this paper reveals the different indication significances of sediment grain sizes in different time scales, different resolution investigations. For long time-scale and low resolution (102a or 103a) studies, larger sediment grain size indicates lower lake level, smaller lake area and drier climate while smaller sediment grain size indicates higher lake level, larger lake area and wetter climate. For short time-scale and high resolution (a or 10a) studies, larger sediment grain size reflects more rainfall and wetter climate while smaller sediment grain size reflects less rainfall and drier climate. Environmental information revealed by lacustrine sediment records is often different in different time scales because of the variance of sedimentation resolution, sampling resolution and dating precision. Therefore, paleoclimate implications of environmental proxies in long time-scale and low resolution investigations could not be mechanically applied to short time-scale and high resolution studies. Only after synthetically analyzing the influence manner and extent of all factors on the sediment records in different time scales, can credible conclusions be obtained.

  4. A wavelet based approach to measure and manage contagion at different time scales

    Science.gov (United States)

    Berger, Theo

    2015-10-01

    We decompose financial return series of US stocks into different time scales with respect to different market regimes. First, we examine dependence structure of decomposed financial return series and analyze the impact of the current financial crisis on contagion and changing interdependencies as well as upper and lower tail dependence for different time scales. Second, we demonstrate to which extent the information of different time scales can be used in the context of portfolio management. As a result, minimizing the variance of short-run noise outperforms a portfolio that minimizes the variance of the return series.

  5. Global Exponential Stability of Delayed Cohen-Grossberg BAM Neural Networks with Impulses on Time Scales

    Directory of Open Access Journals (Sweden)

    Fei Yu

    2009-01-01

    Full Text Available Based on the theory of calculus on time scales, the homeomorphism theory, Lyapunov functional method, and some analysis techniques, sufficient conditions are obtained for the existence, uniqueness, and global exponential stability of the equilibrium point of Cohen-Grossberg bidirectional associative memory (BAM neural networks with distributed delays and impulses on time scales. This is the first time applying the time-scale calculus theory to unify the discrete-time and continuous-time Cohen-Grossberg BAM neural network with impulses under the same framework.

  6. A Study on Time-Scales Ratio and Turbulent Prandtl Number in Ducts of Industrial Applications

    DEFF Research Database (Denmark)

    Rokni, Masoud

    2006-01-01

    This investigation concerns numerical time-scales ratio and turbulent Prandtl number in fully developed turbulent flows in ducts of various cross-sections. The low Reynolds number version of a non-linear eddy viscosity model is proposed to predict the Reynolds stresses and the temperature field...... is solved using a two-equation heat flux model. The computed results compare satisfactory with the available experimental data. The time-scale ratio R is defined as the ratio between the dynamic time-scale (k/ε) and the scalar time-scale(0.5θθ/εθ). Based on existing DNS data and calculations in this work...

  7. Existence Results for Higher-Order Boundary Value Problems on Time Scales

    OpenAIRE

    Sang Yanbin; Liu Jian

    2009-01-01

    By using the fixed-point index theorem, we consider the existence of positive solutions for the following nonlinear higher-order four-point singular boundary value problem on time scales , ; , ; , ; , , where , , , , , , , and is rd-continuous.

  8. Multi-time scale analysis of precipitation variation in Guyuan,China:1957-2005

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Morlet wavelet transformation is used in this paper to analyze the multi-time scale characteristics of precipitation data series from 1957 to 2005 in Guyuan region.The results showed that (1) the annual precipitation evolution process had obvious multi-time scale variation characteristics of 15-25 years,7-12 years and 3-6 years,and different time scales had different oscillation energy densities;(2) the periods at smaller time scales changed more frequently,which often nested in a biggish quasi periodic oscillations,so the concrete time domain should be analyzed if necessary;(3) the precipitation had three main periods (22-year,9-year and 4-year) and the 22-year period was especially outstanding,and the analysis of this main period reveals that the precipitation would be in a relative high water period until about 2012.

  9. Time-scale invariances in preseismic electromagnetic radiation, magnetization and damage evolution of rocks

    Directory of Open Access Journals (Sweden)

    Y. Kawada

    2007-10-01

    Full Text Available We investigate the time-scale invariant changes in electromagnetic and mechanical energy releases prior to a rock failure or a large earthquake. The energy release processes are caused by damage evolutions such as crack propagation, motion of charged dislocation, area-enlargement of sheared asperities and repetitive creep-rate changes. Damage mechanics can be used to represent the time-scale invariant evolutions of both brittle and plastic damages. Irreversible thermodynamics applied to the damage mechanics reveals that the damage evolution produces the variations in charge, dipole and electromagnetic signals in addition to mechanical energy release, and yields the time-scale invariant patterns of Benioff electromagnetic radiation and cumulative Benioff strain-release. The irreversible thermodynamic framework of damage mechanics is also applicable to the seismo-magnetic effect, and the time-scale invariance is recognized in the remanent magnetization change associated with damage evolution prior to a rock failure.

  10. Monotone Iterative Technique for Partial Dynamic Equations of First Order on Time Scales

    Directory of Open Access Journals (Sweden)

    Peiguang Wang

    2008-01-01

    Full Text Available This work is concerned with the monotone iterative technique for partial dynamic equations of first order on time scales and for this purpose, the existence, uniqueness, and comparison results are also established.

  11. The actor-critic algorithm as multi-time-scale stochastic approximation

    OpenAIRE

    Borkar, Vivek S.; Konda, Vijaymohan R

    1997-01-01

    The actor-critic algorithm of Barto and others for simulation-based optimization of Markov decision processes is cast as a two time Scale stochastic approximation. Convergence analysis, approximation issues and an example are studied.

  12. Monotone Iterative Technique for First-Order Nonlinear Periodic Boundary Value Problems on Time Scales

    Directory of Open Access Journals (Sweden)

    Zhao Ya-Hong

    2010-01-01

    Full Text Available We investigate the following nonlinear first-order periodic boundary value problem on time scales: , , . Some new existence criteria of positive solutions are established by using the monotone iterative technique.

  13. OSCILLATION CRITERIA FOR A FOURTH ORDER SUBLINEAR DYNAMIC EQUATION ON TIME SCALE

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Some new criteria for the oscillation of a fourth order sublinear and/or linear dynamic equation on time scale are established. Our results are new for the corresponding fourth order differential equations as well as difference equations.

  14. Empirical study on structural properties in temporal networks under different time scales

    Science.gov (United States)

    Chen, Duanbing

    2015-12-01

    Many network analyzing methods are usually based on static networks. However, temporal networks should be considered so as to investigate real complex systems deeply since some dynamics on these systems cannot be described by static networks accurately. In this paper, four structural properties in temporal networks are empirically studied, including degree, clustering coefficient, adjacent correlation, and connected component. Three real temporal networks with different time scales are analyzed in this paper, including short message, telephone, and router networks. Moreover, structural properties of these temporal networks are compared with that of corresponding static aggregation networks in the whole time window. Some essential differences of structural properties between temporal and static networks are achieved through empirical analysis. Finally, the effect of structural properties on spreading dynamics under different time scales is investigated. Some interesting results such as turning point of structure evolving time scale corresponding to certain spreading dynamics time scale from the point of view of infected scale are achieved.

  15. Existence and exponential stability of positive almost periodic solution for Nicholson's blowflies models on time scales.

    Science.gov (United States)

    Li, Yongkun; Li, Bing

    2016-01-01

    In this paper, we first give a new definition of almost periodic time scales, two new definitions of almost periodic functions on time scales and investigate some basic properties of them. Then, as an application, by using a fixed point theorem in Banach space and the time scale calculus theory, we obtain some sufficient conditions for the existence and exponential stability of positive almost periodic solutions for a class of Nicholson's blowflies models on time scales. Finally, we present an illustrative example to show the effectiveness of obtained results. Our results show that under a simple condition the continuous-time Nicholson's blowflies model and its discrete-time analogue have the same dynamical behaviors. PMID:27468397

  16. ON GLOBAL ROBUST STABILITY FOR COMPETITIVE NEURAL NETWORKS WITH TIME DELAYS AND DIFFERENT TIME SCALES

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    In this paper, using the theory of topological degree and Liapunov functional methods, the authors study the competitive neural networks with time delays and different time scales and present some criteria of global robust stability for this neural network model.

  17. Gas Chromatography.

    Science.gov (United States)

    Karasek, Francis W.; And Others

    1984-01-01

    This review covers fundamental developments in gas chromatography during 1982 and 1983. Literature is considered under these headings: columns; liguid phases; solid supports; sorption processes and solvents; open tubular column gas chromatography; instrumentation; high-resolution columns and applications; other techniques; qualitative and…

  18. Global Uniform Asymptotic Stability of Competitive Neural Networks with Different-Time Scales and Delay

    Institute of Scientific and Technical Information of China (English)

    LI Hong; L(U) Shu; ZHONG Shou-ming

    2005-01-01

    The global uniform asymptotic stability of competitive neural networks with different time scales and delay is investigated. By the method of variation of parameters and the method of inequality analysis, the condition for global uniformly asymptotically stable are given. A strict Lyapunov function for the flow of a competitive neural system with different time scales and delay is presented. Based on the function, the global uniform asymptotic stability of the equilibrium point can be proved.

  19. Combined use of meteorological drought indices at multi-time scales for improving hydrological drought detection.

    Science.gov (United States)

    Zhu, Ye; Wang, Wen; Singh, Vijay P; Liu, Yi

    2016-11-15

    Prediction of hydrological drought in the absence of hydrological records is of great significance for water resources management and risk assessment. In this study, two meteorological drought indices, including standardized precipitation index (SPI) and standardized precipitation evapotranspiration index (SPEI) calculated at different time scales (1 to 12months), were analyzed for their capabilities in detecting hydrological droughts. The predictive skills of meteorological drought indices were assessed through correlation analysis, and two skill scores, i.e. probability of detection (POD) and false alarm rate (FAR). When used independently, indices of short time scales generally performed better than did those of long time scales. However, at least 31% of hydrological droughts were still missed in view of the peak POD score (0.69) of a single meteorological drought index. Considering the distinguished roles of different time scales in explaining hydrological droughts with disparate features, an optimization approach of blending SPI/SPEI at multiple time scales was proposed. To examine the robustness of the proposed method, data of 1964-1990 was used to establish the multiscalar index, then validate during 2000-2010. Results showed that POD exhibited a significant increase when more than two time scales were used, and the best performances were found when blending 8 time scales of SPI and 9 for SPEI, with the corresponding values of 0.82 and 0.85 for POD, 0.205 and 0.21 for FAR, in the calibration period, and even better performance in the validation period. These results far exceeded the performance of any single meteorological drought index. This suggests that when there is lack of streamflow measurements, blending climatic information of multiple time scales to jointly monitor hydrological droughts could be an alternative solution.

  20. Interplay between multiple length and time scales in complex chemical systems

    OpenAIRE

    Bagchi, Biman; Chakravarty, Charusita

    2010-01-01

    Processes in complex chemical systems, such as macromolecules, electrolytes, interfaces, micelles and enzymes, can span several orders of magnitude in length and time scales. The length and time scales of processes occurring over this broad time and space window are frequently coupled to give rise to the control necessary to ensure specificity and the uniqueness of the chemical phenomena. A combination of experimental, theoretical and computational techniques that can address a multiplicity o...

  1. Oscillation of Second-order Nonlinear Dynamic Equation on Time Scales

    Institute of Scientific and Technical Information of China (English)

    YANG Jia-shan

    2013-01-01

    The oscillation for a class of second order nonlinear variable delay dynamic equation on time scales with nonlinear neutral term and damping term was discussed in this article.By using the generalized Riccati technique,integral averaging technique and the time scales theory,some new sufficient conditions for oscillation of the equation are proposed.These results generalize and extend many known results for second order dynamic equations.Some examples are given to illustrate the main results of this article.

  2. Insights from inside the spinodal: Bridging thermalization time scales with smoothed particle hydrodynamics

    Science.gov (United States)

    Pütz, Martin; Nielaba, Peter

    2016-08-01

    We report the influence of the strength of heat bath coupling on the demixing behavior in spinodal decomposing one component liquid-vapor systems. The smoothed particle hydrodynamics (SPH) method with a van der Waals equation of state is used for the simulation. A thermostat for SPH is introduced that is based on the Berendsen thermostat. It controls the strength of heat bath coupling and allows for quenches with exponential temperature decay at a certain thermalization time scale. The present method allows us to bridge several orders of magnitude in the thermalization time scale. The early stage is highly affected by the choice of time scale. A transition from exponential growth to a 1 /2 ordinary power law scaling in the characteristic lengths is observed. At high initial temperatures the growth is logarithmic. The comparison with pure thermal simulations reveals latent heat to raise the mean system temperature. Large thermalization time scales and thermal conductivity are figured out to affect a stagnation of heating, which is explained with convective processes. Furthermore, large thermalization time scales are responsible for a stagnation of growth of domains, which is temporally embedded between early and late stage of phase separation. Therefore, it is considered as an intermediate stage. We present an aspect concerning this stage, namely that choosing larger thermalization time scales increases the duration. Moreover, it is observed that diffuse interfaces are formed during this stage, provided that the stage is apparent. We show that the differences in the evolution between pure thermal simulations and simulations with an instantaneously scaled mean temperature can be explained by the thermalization process, since a variation of the time scale allows for the bridging between these cases of limit.

  3. Insights from inside the spinodal: Bridging thermalization time scales with smoothed particle hydrodynamics.

    Science.gov (United States)

    Pütz, Martin; Nielaba, Peter

    2016-08-01

    We report the influence of the strength of heat bath coupling on the demixing behavior in spinodal decomposing one component liquid-vapor systems. The smoothed particle hydrodynamics (SPH) method with a van der Waals equation of state is used for the simulation. A thermostat for SPH is introduced that is based on the Berendsen thermostat. It controls the strength of heat bath coupling and allows for quenches with exponential temperature decay at a certain thermalization time scale. The present method allows us to bridge several orders of magnitude in the thermalization time scale. The early stage is highly affected by the choice of time scale. A transition from exponential growth to a 1/2 ordinary power law scaling in the characteristic lengths is observed. At high initial temperatures the growth is logarithmic. The comparison with pure thermal simulations reveals latent heat to raise the mean system temperature. Large thermalization time scales and thermal conductivity are figured out to affect a stagnation of heating, which is explained with convective processes. Furthermore, large thermalization time scales are responsible for a stagnation of growth of domains, which is temporally embedded between early and late stage of phase separation. Therefore, it is considered as an intermediate stage. We present an aspect concerning this stage, namely that choosing larger thermalization time scales increases the duration. Moreover, it is observed that diffuse interfaces are formed during this stage, provided that the stage is apparent. We show that the differences in the evolution between pure thermal simulations and simulations with an instantaneously scaled mean temperature can be explained by the thermalization process, since a variation of the time scale allows for the bridging between these cases of limit. PMID:27627369

  4. Time-scales of close-in exoplanet radio emission variability

    CERN Document Server

    See, V; Fares, R; Donati, J -F; Moutou, C

    2015-01-01

    We investigate the variability of exoplanetary radio emission using stellar magnetic maps and 3D field extrapolation techniques. We use a sample of hot Jupiter hosting stars, focusing on the HD 179949, HD 189733 and tau Boo systems. Our results indicate two time-scales over which radio emission variability may occur at magnetised hot Jupiters. The first is the synodic period of the star-planet system. The origin of variability on this time-scale is the relative motion between the planet and the interplanetary plasma that is co-rotating with the host star. The second time-scale is the length of the magnetic cycle. Variability on this time-scale is caused by evolution of the stellar field. At these systems, the magnitude of planetary radio emission is anticorrelated with the angular separation between the subplanetary point and the nearest magnetic pole. For the special case of tau Boo b, whose orbital period is tidally locked to the rotation period of its host star, variability only occurs on the time-scale of...

  5. Micro- and nano- second time scale, high power electrical wire explosions in water.

    Science.gov (United States)

    Grinenko, Alon; Efimov, Sergey; Sayapin, Arkadii; Fedotov, Alexander; Gurovich, Viktor; Krasik, Yakov

    2006-10-01

    Experimental and magneto-hydro-dynamic simulation results of micro- and nanosecond time scale underwater electrical Al, Cu and W wires explosions are presented. A capacitor bank with stored energy up to 6 kJ (discharge current up to 80 kA with 2.5 μs quarter period) was used in microsecond time scale experiments and water forming line generator with current amplitude up to 100 kA and pulse duration of 100 ns were used in nanosecond time scale experiments. Extremely high energy deposition of up to 60 times the atomization enthalpy was registered in nanosecond time scale explosions. A discharge channel evolution and surface temperature were analyzed by streak shadow imaging and using fast photo-diode with a set of interference filters, respectively. Microsecond time scale electrical explosion of cylindrical wire array showed extremely high pressure of converging shock waves at the axis, up to 0.2 MBar. A 1D and 2D magneto-hydro-dynamic simulation demonstrated good agreement with such experimental parameters as discharge channel current, voltage, radius, and temperature.

  6. New time-scale criteria for model simplification of bio-reaction systems

    Directory of Open Access Journals (Sweden)

    Lee Sang

    2008-08-01

    Full Text Available Abstract Background Quasi-steady state approximation (QSSA based on time-scale analysis is known to be an effective method for simplifying metabolic reaction system, but the conventional analysis becomes time-consuming and tedious when the system is large. Although there are automatic methods, they are based on eigenvalue calculations of the Jacobian matrix and on linear transformations, which have a high computation cost. A more efficient estimation approach is necessary for complex systems. Results This work derived new time-scale factor by focusing on the problem structure. By mathematically reasoning the balancing behavior of fast species, new time-scale criteria were derived with a simple expression that uses the Jacobian matrix directly. The algorithm requires no linear transformation or decomposition of the Jacobian matrix, which has been an essential part for previous automatic time-scaling methods. Furthermore, the proposed scale factor is estimated locally. Therefore, an iterative procedure was also developed to find the possible multiple boundary layers and to derive an appropriate reduced model. Conclusion By successive calculation of the newly derived time-scale criteria, it was possible to detect multiple boundary layers of full ordinary differential equation (ODE models. Besides, the iterative procedure could derive the appropriate reduced differential algebraic equation (DAE model with consistent initial values, which was tested with simple examples and a practical example.

  7. Interplay between multiple length and time scales in complex chemical systems

    Indian Academy of Sciences (India)

    Biman Bagchi; Charusita Chakravarty

    2010-07-01

    Processes in complex chemical systems, such as macromolecules, electrolytes, interfaces, micelles and enzymes, can span several orders of magnitude in length and time scales. The length and time scales of processes occurring over this broad time and space window are frequently coupled to give rise to the control necessary to ensure specificity and the uniqueness of the chemical phenomena. A combination of experimental, theoretical and computational techniques that can address a multiplicity of length and time scales is required in order to understand and predict structure and dynamics in such complex systems. This review highlights recent experimental developments that allow one to probe structure and dynamics at increasingly smaller length and time scales. The key theoretical approaches and computational strategies for integrating information across time-scales are discussed. The application of these ideas to understand phenomena in various areas, ranging from materials science to biology, is illustrated in the context of current developments in the areas of liquids and solvation, protein folding and aggregation and phase transitions, nucleation and self-assembly.

  8. Long-time scale spectral diffusion in PMMA: Beyond the TLS model?

    Science.gov (United States)

    Müller, J.; Haarer, D.; Khodykin, O. V.; Kharlamov, B. M.

    1999-05-01

    Spectral diffusion (SD) in PMMA doped with H 2-TPP is investigated at 4.2 K on a time scale of 3 ÷ 10 6 s via optical hole burning. Two contradictory (in frames of the TLS model) results are obtained. The first is the absence of aging effects which put the upper limit for the TLS relaxation times to tens of minutes. The second is an intensive superlogarithmic SD on the whole time scale of the experiment, which evidences the presence of very slow relaxations, independent of the sample history on the time scale of up to 2 months. The presented results provide the clear evidence of the deviation of SD behavior from the TLS model predictions at moderately low temperatures. The concept of structural relaxations is applied for a qualitative interpretation of the experimental data.

  9. Multiple-Time-Scales Hierarchical Frequency Stability Control Strategy of Medium-Voltage Isolated Microgrid

    DEFF Research Database (Denmark)

    Zhao, Zhuoli; Yang, Ping; Guerrero, Josep M.;

    2016-01-01

    . In this way, dynamic stability control that cope with disturbances in short-time scale is implemented by microgrid central controller (MGCC) within Zone B and Zone C. Meanwhile, steady-state stability control to solve the peaks and valleys problem of loads and DGs in long-time scale is executed by microgrid......, it is more complex to control and manage. Thus in order to maintain the frequency stability in multiple-time-scales, a hierarchical control strategy is proposed. The proposed control architecture divides the system frequency in three zones: (A) stable zone, (B) precautionary zone and (C) emergency zone...... energy management system (MEMS) within Zone A. Furthermore, based on the developed complete small-signal state-space model, sensitivity analysis of the eigenvalues is conducted in order to reveal the dynamic stability margin of the MV microgrid, and to identify the proper range of the control parameters...

  10. Predicting Regional Drought on Sub-Seasonal to Decadal Time Scales

    Science.gov (United States)

    Schubert, Siegfried; Wang, Hailan; Suarez, Max; Koster, Randal

    2011-01-01

    Drought occurs on a wide range of time scales, and within a variety of different types of regional climates. It is driven foremost by an extended period of reduced precipitation, but it is the impacts on such quantities as soil moisture, streamflow and crop yields that are often most important from a users perspective. While recognizing that different users have different needs for drought information, it is nevertheless important to understand that progress in predicting drought and satisfying such user needs, largely hinges on our ability to improve predictions of precipitation. This talk reviews our current understanding of the physical mechanisms that drive precipitation variations on subseasonal to decadal time scales, and the implications for predictability and prediction skill. Examples are given highlighting the phenomena and mechanisms controlling precipitation on monthly (e.g., stationary Rossby waves, soil moisture), seasonal (ENSO) and decadal time scales (PD and AMO).

  11. Analytical expression for gas-particle equilibration time scale and its numerical evaluation

    Science.gov (United States)

    Anttila, Tatu; Lehtinen, Kari E. J.; Dal Maso, Miikka

    2016-05-01

    We have derived a time scale τeq that describes the characteristic time for a single compound i with a saturation vapour concentration Ceff,i to reach thermodynamic equilibrium between the gas and particle phases. The equilibration process was assumed to take place via gas-phase diffusion and absorption into a liquid-like phase present in the particles. It was further shown that τeq combines two previously derived and often applied time scales τa and τs that account for the changes in the gas and particle phase concentrations of i resulting from the equilibration, respectively. The validity of τeq was tested by comparing its predictions against results from a numerical model that explicitly simulates the transfer of i between the gas and particle phases. By conducting a large number of simulations where the values of the key input parameters were varied randomly, it was found out that τeq yields highly accurate results when i is a semi-volatile compound in the sense that the ratio of total (gas and particle phases) concentration of i to the saturation vapour concentration of i, μ, is below unity. On the other hand, the comparison of analytical and numerical time scales revealed that using τa or τs alone to calculate the equilibration time scale may lead to considerable errors. It was further shown that τeq tends to overpredict the equilibration time when i behaves as a non-volatile compound in a sense that μ > 1. Despite its simplicity, the time scale derived here has useful applications. First, it can be used to assess if semi-volatile compounds reach thermodynamic equilibrium during dynamic experiments that involve changes in the compound volatility. Second, the time scale can be used in modeling of secondary organic aerosol (SOA) to check whether SOA forming compounds equilibrate over a certain time interval.

  12. Response of vegetation to drought time-scales across global land biomes.

    Science.gov (United States)

    Vicente-Serrano, Sergio M; Gouveia, Célia; Camarero, Jesús Julio; Beguería, Santiago; Trigo, Ricardo; López-Moreno, Juan I; Azorín-Molina, César; Pasho, Edmond; Lorenzo-Lacruz, Jorge; Revuelto, Jesús; Morán-Tejeda, Enrique; Sanchez-Lorenzo, Arturo

    2013-01-01

    We evaluated the response of the Earth land biomes to drought by correlating a drought index with three global indicators of vegetation activity and growth: vegetation indices from satellite imagery, tree-ring growth series, and Aboveground Net Primary Production (ANPP) records. Arid and humid biomes are both affected by drought, and we suggest that the persistence of the water deficit (i.e., the drought time-scale) could be playing a key role in determining the sensitivity of land biomes to drought. We found that arid biomes respond to drought at short time-scales; that is, there is a rapid vegetation reaction as soon as water deficits below normal conditions occur. This may be due to the fact that plant species of arid regions have mechanisms allowing them to rapidly adapt to changing water availability. Humid biomes also respond to drought at short time-scales, but in this case the physiological mechanisms likely differ from those operating in arid biomes, as plants usually have a poor adaptability to water shortage. On the contrary, semiarid and subhumid biomes respond to drought at long time-scales, probably because plants are able to withstand water deficits, but they lack the rapid response of arid biomes to drought. These results are consistent among three vegetation parameters analyzed and across different land biomes, showing that the response of vegetation to drought depends on characteristic drought time-scales for each biome. Understanding the dominant time-scales at which drought most influences vegetation might help assessing the resistance and resilience of vegetation and improving our knowledge of vegetation vulnerability to climate change.

  13. Global scale precipitation from monthly to centennial scales: empirical space-time scaling analysis, anthropogenic effects

    Science.gov (United States)

    de Lima, Isabel; Lovejoy, Shaun

    2016-04-01

    The characterization of precipitation scaling regimes represents a key contribution to the improved understanding of space-time precipitation variability, which is the focus here. We conduct space-time scaling analyses of spectra and Haar fluctuations in precipitation, using three global scale precipitation products (one instrument based, one reanalysis based, one satellite and gauge based), from monthly to centennial scales and planetary down to several hundred kilometers in spatial scale. Results show the presence - similarly to other atmospheric fields - of an intermediate "macroweather" regime between the familiar weather and climate regimes: we characterize systematically the macroweather precipitation temporal and spatial, and joint space-time statistics and variability, and the outer scale limit of temporal scaling. These regimes qualitatively and quantitatively alternate in the way fluctuations vary with scale. In the macroweather regime, the fluctuations diminish with time scale (this is important for seasonal, annual, and decadal forecasts) while anthropogenic effects increase with time scale. Our approach determines the time scale at which the anthropogenic signal can be detected above the natural variability noise: the critical scale is about 20 - 40 yrs (depending on the product, on the spatial scale). This explains for example why studies that use data covering only a few decades do not easily give evidence of anthropogenic changes in precipitation, as a consequence of warming: the period is too short. Overall, while showing that precipitation can be modeled with space-time scaling processes, our results clarify the different precipitation scaling regimes and further allow us to quantify the agreement (and lack of agreement) of the precipitation products as a function of space and time scales. Moreover, this work contributes to clarify a basic problem in hydro-climatology, which is to measure precipitation trends at decadal and longer scales and to

  14. Valuation of Credit Derivatives with Multiple Time Scales in the Intensity Model

    Directory of Open Access Journals (Sweden)

    Beom Jin Kim

    2014-01-01

    Full Text Available We propose approximate solutions for pricing zero-coupon defaultable bonds, credit default swap rates, and bond options based on the averaging principle of stochastic differential equations. We consider the intensity-based defaultable bond, where the volatility of the default intensity is driven by multiple time scales. Small corrections are computed using regular and singular perturbations to the intensity of default. The effectiveness of these corrections is tested on the bond price and yield curve by investigating the behavior of the time scales with respect to the relevant parameters.

  15. Doubly stochastic Poisson process models for precipitation at fine time-scales

    Science.gov (United States)

    Ramesh, Nadarajah I.; Onof, Christian; Xie, Dichao

    2012-09-01

    This paper considers a class of stochastic point process models, based on doubly stochastic Poisson processes, in the modelling of rainfall. We examine the application of this class of models, a neglected alternative to the widely-known Poisson cluster models, in the analysis of fine time-scale rainfall intensity. These models are mainly used to analyse tipping-bucket raingauge data from a single site but an extension to multiple sites is illustrated which reveals the potential of this class of models to study the temporal and spatial variability of precipitation at fine time-scales.

  16. Application of Time Scale to Parameters Tuning of Active Disturbance Rejection Controller for Induction Motor

    Institute of Scientific and Technical Information of China (English)

    SHAO Li-wei; LIAO Xiao-zhong; ZHANG Yu-he

    2007-01-01

    Active disturbance rejection controller (ADRC) has good performance in induction motor (IM) control system, but controller parameter is difficult to tune. A method of tuning ADRC parameter by time scale is analyzed. The IM time scale is obtained by theoretical analysis. Combining the relations between scale time and ADRC parameters, ADRC parameter tuning in IM vector control based stator flux oriented is obtained. This parameter tuning method is validated by simulations and it provides a new technique for tuning of ADRC parameters of IM.

  17. Time-Scales for Non-Inductive Current Buildup in Low-Aspect-Ratio Toroidal Geometry

    International Nuclear Information System (INIS)

    The fundamental differences between inductive and non-inductive current buildup are clarified and the associated time-scales and other implications are discussed. A simulation is presented whereby the plasma current in a low-aspect-ratio torus is increased primarily by the self-generated bootstrap current with only 10% coming from external current drive. The maximum obtainable plasma current by this process is shown to scale with the toroidal field strength. The basic physics setting the time-scales can be obtained from a 1D analysis. Comparisons are made between the timescales found here and those reported in the experimental literature

  18. Separation of time-scales and reparametrization invariance for aging systems

    International Nuclear Information System (INIS)

    We show that the generating functional describing the slow dynamics of spin glass systems is invariant under reparametrizations of the time. This result is general and applies for both infinite and short range models. It follows simply from the assumption that a separation between short time-scales and long time-scales exists in the system, and the constraints of causality and unitarity. Global time reparametrization invariance suggests that the low action excitations in a spin glass may be smoothly spatially varying time reparametrizations. These Goldstone modes may provide the basis for an analytic dynamical theory of short range spin glasses. (author)

  19. A new Geologic Time Scale, with special reference to Precambrian and Neogene

    Institute of Scientific and Technical Information of China (English)

    FelixM.Gradstein; JamesG.Ogg; AlanG.Smith; WouterBleeker; LucasJ.Lourens

    2004-01-01

    A Geologic Time Scale (GTS2004) is presented that integrates currently available stratigraphic and geochronologic information. Key features of the new scale are outlined, how it was constructed, and how it can be further improved. The accompanying International Stratigraphic Chart, issued under auspices of the International Commission on Stratigraphy (ICS), shows the current chronostratigraphic scale and ages with estimates of uncertainty for all stage boundaries. Special reference is made to the Precambrian part of the time scale, which is coming of age in terms of detail, and to the Neogene portion, which has attained an ultra-high-precision absolute-age calibration.

  20. On Qualitative Analysis of Delay Systems and $x^ = f (t, x, x^)$ on Time Scales

    Indian Academy of Sciences (India)

    Yajun Ma; Yu Zhang; Jitao Sun

    2010-04-01

    Here we solve two problems presented in paper [9] (C C Tisdell and A Zaidi, Basic qualitative and quantitative results for solutions to nonlinear, dynamic equations on time scales with an application to economic modelling, Nonlinear Anal. 68 (2008) 3504–3524). We study existence and uniqueness of solutions for delay systems and first-order dynamic equations of the form $x^=f(t,x,x^)$ on time scales by using the Banach’s fixed-point theorem. Some examples are presented to illustrate the efficiency of the proposed results.

  1. Arctic energy budget in relation to sea ice variability on monthly-to-annual time scales

    NARCIS (Netherlands)

    Krikken, F.; Hazeleger, W.

    2015-01-01

    The large decrease in Arctic sea ice in recent years has triggered a strong interest in Arctic sea ice predictions on seasonal-to-decadal time scales. Hence, it is important to understand physical processes that provide enhanced predictability beyond persistence of sea ice anomalies. This study anal

  2. Fixation of competing strategies when interacting agents differ in the time scale of strategy updating

    Science.gov (United States)

    Zhang, Jianlei; Weissing, Franz J.; Cao, Ming

    2016-09-01

    A commonly used assumption in evolutionary game theory is that natural selection acts on individuals in the same time scale; e.g., players use the same frequency to update their strategies. Variation in learning rates within populations suggests that evolutionary game theory may not necessarily be restricted to uniform time scales associated with the game interaction and strategy adaption evolution. In this study, we remove this restricting assumption by dividing the population into fast and slow groups according to the players' strategy updating frequencies and investigate how different strategy compositions of one group influence the evolutionary outcome of the other's fixation probabilities of strategies within its own group. Analytical analysis and numerical calculations are performed to study the evolutionary dynamics of strategies in typical classes of two-player games (prisoner's dilemma game, snowdrift game, and stag-hunt game). The introduction of the heterogeneity in strategy-update time scales leads to substantial changes in the evolution dynamics of strategies. We provide an approximation formula for the fixation probability of mutant types in finite populations and study the outcome of strategy evolution under the weak selection. We find that although heterogeneity in time scales makes the collective evolutionary dynamics more complicated, the possible long-run evolutionary outcome can be effectively predicted under technical assumptions when knowing the population composition and payoff parameters.

  3. POSITIVE SOLUTIONS TO A SECOND-ORDER m-POINT BOUNDARY VALUE PROBLEM ON TIME SCALES

    Institute of Scientific and Technical Information of China (English)

    Liu Yang; Chunfang Shen

    2009-01-01

    By a fixed point theorem in a cone,the existence of at least three positive solutions to a class of second-order multi-point boundary value problem for dynamic equation on time scales with the nonlinear term depends on the first order derivative is studied.

  4. ASYMPTOTIC BEHAVIOR OF SOLUTIONS TO SECOND ORDER IMPULSIVE DIFFERENTIAL EQUATION ON TIME SCALES

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    In this paper,we investigate a second order impulsive differential equation on time scales.Sufficient conditions are given to guarantee that the solutions tend to zero.The notable effect of impulse upon the asymptotic behavior of solutions is stressed in this paper.At last,we illustrate our results with two examples.

  5. Time scales: from Nabla calculus to Delta calculus and vice versa via duality

    OpenAIRE

    Caputo, M. Cristina

    2009-01-01

    In this note we show how one can obtain results from the nabla calculus from results on the delta calculus and vice versa via a duality argument. We provide applications of the main results to the calculus of variations on time scales.

  6. A limit set trichotomy for order-preserving systems on time scales

    Directory of Open Access Journals (Sweden)

    Christian Poetzsche

    2004-04-01

    Full Text Available In this paper we derive a limit set trichotomy for abstract order-preserving 2-parameter semiflows in normal cones of strongly ordered Banach spaces. Additionally, to provide an example, Muller's theorem is generalized to dynamic equations on arbitrary time scales and applied to a model from population dynamics.

  7. Influence of the time scale on the construction of financial networks.

    Directory of Open Access Journals (Sweden)

    Frank Emmert-Streib

    Full Text Available BACKGROUND: In this paper we investigate the definition and formation of financial networks. Specifically, we study the influence of the time scale on their construction. METHODOLOGY/PRINCIPAL FINDINGS: For our analysis we use correlation-based networks obtained from the daily closing prices of stock market data. More precisely, we use the stocks that currently comprise the Dow Jones Industrial Average (DJIA and estimate financial networks where nodes correspond to stocks and edges correspond to none vanishing correlation coefficients. That means only if a correlation coefficient is statistically significant different from zero, we include an edge in the network. This construction procedure results in unweighted, undirected networks. By separating the time series of stock prices in non-overlapping intervals, we obtain one network per interval. The length of these intervals corresponds to the time scale of the data, whose influence on the construction of the networks will be studied in this paper. CONCLUSIONS/SIGNIFICANCE: Numerical analysis of four different measures in dependence on the time scale for the construction of networks allows us to gain insights about the intrinsic time scale of the stock market with respect to a meaningful graph-theoretical analysis.

  8. Space-time scaling invariant traveling wave solutions of some nonlinear fractional equations

    OpenAIRE

    HE, Tianlan; Fang, Hui

    2012-01-01

    In this paper, a homogeneous principle is proposed to seek the space-time scaling invariant traveling wave solutions expressed by power functions for some fractional differential equations. Applying this principle to generalized fractional Benjamin-Ono equations and generalized fractional ZakharovKuznetsov equations, the traveling wave solutions expressed by power functions have been obtained under some parameter conditions.

  9. Fission time-scale from the measurement of pre-scission light particles and -ray multiplicities

    Indian Academy of Sciences (India)

    K Ramachandran; A Chatterjee; A Navin; K Mahata; A Shrivastava; V Tripathi; S Kailas; V Nanal; R G Pillay; A Saxena; R G Thomas; D R Chakrabarty; V M Datar; Suresh Kumar; P K Sahu

    2015-08-01

    An overview of the experimental result on simultaneous measurement of pre-scission neutron, proton, -particle and GDR -ray multiplicities for the reaction 28Si+175Lu at 159 MeV using the BARC–TIFR Pelletron–LINAC accelerator facility is given. The data were analysed using deformation-dependent particle transmission coefficients, binding energies and level densities which are incorporated in the code JOANNE2 to extract fission time-scales and mean deformation of the saddle-to-scission emitter. The neutron, light charged particle and GDR -ray multiplicity data could be explained consistently. The emission of neutrons seems to be favoured towards larger deformation as compared to charged particles. The pre-saddle time-scale is deduced as (0–2) × 10−21 s whereas the saddle-to-scission time-scale is (36–39) × 10−21 s. The total fission time-scale is deduced as (36–41) × 10−21 s.

  10. PERIODIC BOUNDARY VALUE PROBLEM FOR NONLINEAR INTEGRO-DIFFERENTIAL EQUATION OF MIXED TYPE ON TIME SCALES

    Institute of Scientific and Technical Information of China (English)

    Yepeng Xing; Qiong Wang; Valery G. Romanovski

    2009-01-01

    We prove several new comparison results and develop the monotone iterative tech-nique to show the existence of extremal solutions to a kind of periodic boundary value problem (PBVP) for nonlinear integro-differential equation of mixed type on time scales.

  11. Hardy inequality on time scales and its application to half-linear dynamic equations

    Directory of Open Access Journals (Sweden)

    Řehák Pavel

    2005-01-01

    Full Text Available A time-scale version of the Hardy inequality is presented, which unifies and extends well-known Hardy inequalities in the continuous and in the discrete setting. An application in the oscillation theory of half-linear dynamic equations is given.

  12. Time scale of scour around a pile in combined waves and current

    DEFF Research Database (Denmark)

    Petersen, Thor Ugelvig; Sumer, B. Mutlu; Fredsøe, Jørgen

    The time scale of the scour process around a circular vertical pile is studied in combined waves and current. A series of tests were carried out in a flume with pile diameters 40 mm and 75 mm, in both steady current, waves and combined waves and current. In the combined wave and current flow regime...

  13. OSCILLATION FOR NONLINEAR SECOND-ORDER DYNAMIC EQUATIONS ON TIME SCALES

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Through the use of generalized Riccati transformation techniques, we establish some oscillation criteria for one type of nonlinear dynamic equation on time scales. Several examples, including a semilinear dynamic equation and a nonlinear Emden-Fowler dynamic equation, are also given to illustrate these criteria and to improve the results obtained in some references.

  14. Mass action realizations of reaction kinetic system models on various time scales

    Energy Technology Data Exchange (ETDEWEB)

    Hangos, K M; Szederkenyi, G, E-mail: hangos@scl.sztaki.hu, E-mail: szeder@scl.sztaki.hu [Process Control Research Group, Computer and Automation Reseach Institute, Kende u. 13-17, H-1111 Budapest (Hungary)

    2011-01-01

    Complex chemical reaction networks often exhibit different dynamic behaviour on different time scales. A combined approach is proposed in this work for determining physically meaningful mass action realizations of complex chemical reaction networks that describe its dynamic behaviour on different time scales. This is achieved by appropriately reducing the detailed overall mass action kinetic scheme using quasi steady state assumptions fit to the particular time scale, and then searching for an optimal realization using mixed integer linear programing. Furthermore, the relationship between the properties (reversibility, deficiency, stability) of the obtained realizations of the same system on different time scales are also investigated and related to the same properties of the detailed overall model. It is shown that the reduced models obtained by quasi steady state assumptions may show exotic nonlinear behaviour, such as oscillations, when the original detailed is globally asymptotically stable. The proposed methods are illustrated by using a simple Michaelis-Menten type reaction kinetic example. The simplified versions of the well known Brusselator model have also been investigated and presented as a case study.

  15. Extension of the astronomically calibrated (polarity) time scale to the Miocene/Pliocene boundary

    NARCIS (Netherlands)

    Hilgen, F.J.

    1991-01-01

    The early Pleistocene to late Pliocene astronormcally calibrated time scale of Shackleton et al. [1] and Hllgen [2] is extended to the Mlocene/Pllocene boundary This is done by correlating the detailed record of CaCO 3 cycles in the Trubl and the lower part of the overlying Narbone Formation (Rossel

  16. Natural variability of atmospheric temperatures and geomagnetic intensity over a wide range of time scales.

    Science.gov (United States)

    Pelletier, Jon D

    2002-02-19

    The majority of numerical models in climatology and geomagnetism rely on deterministic finite-difference techniques and attempt to include as many empirical constraints on the many processes and boundary conditions applicable to their very complex systems. Despite their sophistication, many of these models are unable to reproduce basic aspects of climatic or geomagnetic dynamics. We show that a simple stochastic model, which treats the flux of heat energy in the atmosphere by convective instabilities with random advection and diffusive mixing, does a remarkable job at matching the observed power spectrum of historical and proxy records for atmospheric temperatures from time scales of one day to one million years (Myr). With this approach distinct changes in the power-spectral form can be associated with characteristic time scales of ocean mixing and radiative damping. Similarly, a simple model of the diffusion of magnetic intensity in Earth's core coupled with amplification and destruction of the local intensity can reproduce the observed 1/f noise behavior of Earth's geomagnetic intensity from time scales of 1 (Myr) to 100 yr. In addition, the statistics of the fluctuations in the polarity reversal rate from time scales of 1 Myr to 100 Myr are consistent with the hypothesis that reversals are the result of variations in 1/f noise geomagnetic intensity above a certain threshold, suggesting that reversals may be associated with internal fluctuations rather than changes in mantle thermal or magnetic boundary conditions. PMID:11875208

  17. DIRECTIONAL DERIVATIVE OF VECTOR FIELD AND REGULAR CURVES ON TIME SCALES

    Institute of Scientific and Technical Information of China (English)

    Emin (O)zyilmaz

    2006-01-01

    The general idea in this paper is to study curves of the parametric equations where the parameter varies in a so-called time scale, which may be an arbitrary closed subset of the set of all real numbers. We introduce the directional derivative according to the vector fields.

  18. POSITIVE SOLUTIONS FOR p-LAPLACIAN DYNAMIC EQUATIONS ON TIME SCALES

    Institute of Scientific and Technical Information of China (English)

    Geng Fengjie; Zhu Deming; Li Hongzhi

    2007-01-01

    The three-point boundary value problems of p-Laplacian dynamic equations on time scales are investigated. By using Krasnosel'skii's fixed-point theorem and fixed-point index theorem, criteria are achieved for the existence of at least one, two or 2n positive solutions.Furthermore, some examples are included to illustrate the main theorems.

  19. Mixing and flushing time scales in the Azhikode Estuary, southwest coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Revichandran, C.; Pylee, A.

    Flushing time scales of the Azhikode Estuary, Kerala, India showed pronounced dry season and wet season signals as well as large inter-annual variation. Cumulative flushing time of the estuary varies from 4.8 tide cycles in April to 1.22 tide cycles...

  20. An Inverse Problem of the Calculus of Variations on Arbitrary Time Scales

    OpenAIRE

    Dryl, Monika; Malinowska, Agnieszka B.; Torres, Delfim F. M.

    2014-01-01

    We consider an inverse extremal problem for variational functionals on arbitrary time scales. Using the Euler-Lagrange equation and the strengthened Legendre condition, we derive a general form for a variational functional that attains a local minimum at a given point of the vector space.

  1. The Available Time Scale: Measuring Foster Parents' Available Time to Foster

    Science.gov (United States)

    Cherry, Donna J.; Orme, John G.; Rhodes, Kathryn W.

    2009-01-01

    This article presents a new measure of available time specific to fostering, the Available Time Scale (ATS). It was tested with a national sample of 304 foster mothers and is designed to measure the amount of time foster parents are able to devote to fostering activities. The ATS has excellent reliability, and good support exists for its validity.…

  2. Charting the Transient Radio Sky on Sub-Second Time-Scales with LOFAR

    NARCIS (Netherlands)

    J.W.T. Hessels

    2011-01-01

    The LOw Frequency ARray (LOFAR) is a radio interferometric telescope that promises to open a largely unexplored window on transient sources in the "radio sky", from time-scales of nanoseconds to years. An important aspect of this will be the study of radio-emitting neutron stars in their various inc

  3. QUALITATIVE BEHAVIORS OF LINEAR TIME-INVARIANT DYNAMIC EQUATIONS ON TIME SCALES

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    We investigate the type of singularity and qualitative structure of solutions to a time-invariant linear dynamic system on time scales. The results truly unify the qualitative behaviors of the system on the continuous and discrete times with any step size.

  4. A hybrid procedure for MSW generation forecasting at multiple time scales in Xiamen City, China

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Lilai, E-mail: llxu@iue.ac.cn [Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021 (China); Xiamen Key Lab of Urban Metabolism, Xiamen 361021 (China); Gao, Peiqing, E-mail: peiqing15@yahoo.com.cn [Xiamen City Appearance and Environmental Sanitation Management Office, 51 Hexiangxi Road, Xiamen 361004 (China); Cui, Shenghui, E-mail: shcui@iue.ac.cn [Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021 (China); Xiamen Key Lab of Urban Metabolism, Xiamen 361021 (China); Liu, Chun, E-mail: xmhwlc@yahoo.com.cn [Xiamen City Appearance and Environmental Sanitation Management Office, 51 Hexiangxi Road, Xiamen 361004 (China)

    2013-06-15

    Highlights: ► We propose a hybrid model that combines seasonal SARIMA model and grey system theory. ► The model is robust at multiple time scales with the anticipated accuracy. ► At month-scale, the SARIMA model shows good representation for monthly MSW generation. ► At medium-term time scale, grey relational analysis could yield the MSW generation. ► At long-term time scale, GM (1, 1) provides a basic scenario of MSW generation. - Abstract: Accurate forecasting of municipal solid waste (MSW) generation is crucial and fundamental for the planning, operation and optimization of any MSW management system. Comprehensive information on waste generation for month-scale, medium-term and long-term time scales is especially needed, considering the necessity of MSW management upgrade facing many developing countries. Several existing models are available but of little use in forecasting MSW generation at multiple time scales. The goal of this study is to propose a hybrid model that combines the seasonal autoregressive integrated moving average (SARIMA) model and grey system theory to forecast MSW generation at multiple time scales without needing to consider other variables such as demographics and socioeconomic factors. To demonstrate its applicability, a case study of Xiamen City, China was performed. Results show that the model is robust enough to fit and forecast seasonal and annual dynamics of MSW generation at month-scale, medium- and long-term time scales with the desired accuracy. In the month-scale, MSW generation in Xiamen City will peak at 132.2 thousand tonnes in July 2015 – 1.5 times the volume in July 2010. In the medium term, annual MSW generation will increase to 1518.1 thousand tonnes by 2015 at an average growth rate of 10%. In the long term, a large volume of MSW will be output annually and will increase to 2486.3 thousand tonnes by 2020 – 2.5 times the value for 2010. The hybrid model proposed in this paper can enable decision makers to

  5. A hybrid procedure for MSW generation forecasting at multiple time scales in Xiamen City, China

    International Nuclear Information System (INIS)

    Highlights: ► We propose a hybrid model that combines seasonal SARIMA model and grey system theory. ► The model is robust at multiple time scales with the anticipated accuracy. ► At month-scale, the SARIMA model shows good representation for monthly MSW generation. ► At medium-term time scale, grey relational analysis could yield the MSW generation. ► At long-term time scale, GM (1, 1) provides a basic scenario of MSW generation. - Abstract: Accurate forecasting of municipal solid waste (MSW) generation is crucial and fundamental for the planning, operation and optimization of any MSW management system. Comprehensive information on waste generation for month-scale, medium-term and long-term time scales is especially needed, considering the necessity of MSW management upgrade facing many developing countries. Several existing models are available but of little use in forecasting MSW generation at multiple time scales. The goal of this study is to propose a hybrid model that combines the seasonal autoregressive integrated moving average (SARIMA) model and grey system theory to forecast MSW generation at multiple time scales without needing to consider other variables such as demographics and socioeconomic factors. To demonstrate its applicability, a case study of Xiamen City, China was performed. Results show that the model is robust enough to fit and forecast seasonal and annual dynamics of MSW generation at month-scale, medium- and long-term time scales with the desired accuracy. In the month-scale, MSW generation in Xiamen City will peak at 132.2 thousand tonnes in July 2015 – 1.5 times the volume in July 2010. In the medium term, annual MSW generation will increase to 1518.1 thousand tonnes by 2015 at an average growth rate of 10%. In the long term, a large volume of MSW will be output annually and will increase to 2486.3 thousand tonnes by 2020 – 2.5 times the value for 2010. The hybrid model proposed in this paper can enable decision makers to

  6. Gas chromatography

    Science.gov (United States)

    Guiochon, Georges; Guillemin, Claude L.

    1990-11-01

    Gas chromatography is a powerful separation technique for gas and vapor mixtures. Combining separation and on-line detection permits accurate quantitative analysis of complex mixtures, including traces of compounds down to parts per trillions in some particular cases. The importance of gas chromatography in quality control and process control in the chemical and drug industry, in environmental pollution investigations and in clinical analysis is critical. The principles of the technique are discussed, the main components of a gas chromatograph are described and some idea of the importance of the applications is given.

  7. The Global Monsoon across Time Scales: is there coherent variability of regional monsoons?

    Science.gov (United States)

    Wang, P. X.; Wang, B.; Cheng, H.; Fasullo, J.; Guo, Z. T.; Kiefer, T.; Liu, Z. Y.

    2014-05-01

    Monsoon has earned increasing attention from the climate community since the last century, yet only recently regional monsoons have been recognized as a global system. It remains a debated issue, however, as to what extent and at which time scales the global monsoon can be viewed as a major mode of climate variability. For this purpose a PAGES Working Group (WG) was set up to investigate the concept of the global monsoon and its future research directions. The WG's synthesis is presented here. On the basis of observation and proxy data, the WG found that the regional monsoons can vary coherently, although not perfectly, at various time scales, ranging from interannual, interdecadal, centennial and millennial, up to orbital and tectonics time scales, conforming the global monsoon concept across time scales. Within the global monsoon system each subsystem has its own features depending on its geographic and topographic conditions. Discrimination of global and regional components in the monsoon system is a key to reveal the driving factors of monsoon variations, hence the global monsoon concept helps to enhance our understanding and to improve future projection of the regional monsoons. This paper starts with a historical review of the global monsoon concept in both modern and paleo-climatology, and an assessment of monsoon proxies used in regional and global scales. The main body of the paper is devoted to a summary of observation data at various time scales, providing evidence for the coherent global monsoon system. The paper concludes with a projection of future monsoon shifts into a warming world. The synthesis will be followed by a companying paper to discuss driving mechanisms and outstanding issues in the global monsoon studies.

  8. Time Scale Optimization and the Hunt for Astronomical Cycles in Deep Time Strata

    Science.gov (United States)

    Meyers, Stephen R.

    2016-04-01

    A valuable attribute of astrochronology is the direct link between chronometer and climate change, providing a remarkable opportunity to constrain the evolution of the surficial Earth System. Consequently, the hunt for astronomical cycles in strata has spurred the development of a rich conceptual framework for climatic/oceanographic change, and has allowed exploration of the geologic record with unprecedented temporal resolution. Accompanying these successes, however, has been a persistent skepticism about appropriate astrochronologic testing and circular reasoning: how does one reliably test for astronomical cycles in stratigraphic data, especially when time is poorly constrained? From this perspective, it would seem that the merits and promise of astrochronology (e.g., a geologic time scale measured in ≤400 kyr increments) also serves as its Achilles heel, if the confirmation of such short rhythms defies rigorous statistical testing. To address these statistical challenges in astrochronologic testing, a new approach has been developed that (1) explicitly evaluates time scale uncertainty, (2) is resilient to common problems associated with spectrum confidence level assessment and 'multiple testing', and (3) achieves high statistical power under a wide range of conditions (it can identify astronomical cycles when present in data). Designated TimeOpt (for "time scale optimization"; Meyers 2015), the method employs a probabilistic linear regression model framework to investigate amplitude modulation and frequency ratios (bundling) in stratigraphic data, while simultaneously determining the optimal time scale. This presentation will review the TimeOpt method, and demonstrate how the flexible statistical framework can be further extended to evaluate (and optimize upon) complex sedimentation rate models, enhancing the statistical power of the approach, and addressing the challenge of unsteady sedimentation. Meyers, S. R. (2015), The evaluation of eccentricity

  9. Characterization of foot-and-mouth disease virus's viral peptides with LC-ESI-MS

    International Nuclear Information System (INIS)

    Peptides and proteins play a central role in numerous biological and physiological processes in living organisms. Viral capsid peptides are part of the viruses' outer shell of genetic materials. Viruses are recognized by immune system via capsid peptides. Depending on this property of capsid peptides, prototypes synthetic peptide-based vaccine can be developed. In this work, we synthesized three different viral peptide sequences of foot-and-mouth disease virus with microwave enhanced solid phase synthesis method. These peptides were characterized by using liquid chromatography electro spray interface mass spectrometry (LC-ESI-MS) with electro spray ionization. We briefly describe the essential facts for peptide characterization. (author)

  10. Ion Chromatography.

    Science.gov (United States)

    Mulik, James D.; Sawicki, Eugene

    1979-01-01

    Accurate for the analysis of ions in solution, this form of analysis enables the analyst to directly assay many compounds that previously were difficult or impossible to analyze. The method is a combination of the methodologies of ion exchange, liquid chromatography, and conductimetric determination with eluant suppression. (Author/RE)

  11. Screening of TACE Peptide Inhibitors from Phage Display Peptide Library

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    To obtain the recombinant tumor necrosis factor-α converting enzyme (TACE) ectodomain and use it as a selective molecule for the screening of TACE peptide inhibitors, the cDNA coding catalytic domain (T800) and full-length ectodomain (T1300) of TACE were amplified by RTPCR, and the expression plasmids were constructed by inserting T800 and T1300 into plasmid pET28a and pET-28c respectively. The recombinant T800 and T1300 were induced by IPTG, and SDSPAGE and Western blotting analysis results revealed that T800 and T1300 were highly expressed in the form of inclusion body. After Ni2+-NTA resin affinity chromatography, the recombinant proteins were used in the screening of TACE-binding peptides from phage display peptide library respectively. After 4 rounds of biopanning, the positive phage clones were analyzed by ELISA, competitive inhibition assay and DNA sequencing. A common amino acid sequence (TRWLVYFSRPYLVAT) was found and synthesized. The synthetic peptide could inhibit the TNF-α release from LPS-stimulated human peripheral blood mononuclear cells (PBMC) up to 60.3 %. FACS analysis revealed that the peptide mediated the accumulation of TNF-α on the cell surface. These results demonstrate that the TACE-binding peptide is an effective antagonist of TACE.

  12. Self-assembling of zinc phthalocyanines on ZnO (1010) surface through multiple time scales.

    Science.gov (United States)

    Melis, Claudio; Raiteri, Paolo; Colombo, Luciano; Mattoni, Alessandro

    2011-12-27

    We adopt a hierarchic combination of theoretical methods to study the assembling of zinc phthalocyanines (ZnPcs) on a ZnO (1010) surface through multiple time scales. Atomistic simulations, such as model potential molecular dynamics and metadynamics, are used to study the energetics and short time evolution (up to ∼100 ns) of small ZnPc aggregates. The stability and the lifetime of large clusters is then studied by means of an atomistically informed coarse-grained model using classical molecular dynamics. Finally, the macroscopic time scale clustering phenomenon is studied by Metropolis Monte Carlo algorithms as a function of temperature and surface coverage. We provide evidence that at room temperature the aggregation is likely to occur at sufficiently high coverage, and we characterize the nature, morphology, and lifetime of ZnPc's clusters. We identify the molecular stripes oriented along [010] crystallographic directions as the most energetically stable aggregates.

  13. A Visual Method of Time Scale Determination using a PC for Radio Sources

    Indian Academy of Sciences (India)

    Yong Huang; Jun-Hui Fan; Jing Pan

    2011-03-01

    Variability is one of the extremely observational properties. In the radio bands, variability is caused by the shock in the jet. In this case, emissions increase rapidly following an exponential curve, and then decrease rapidly also in an exponential curve. The variability time scale is important with regard to the physics carrying on in the jet. However, it is not easy to fit the light curve. In this paper, we proposed a method of light curve fitting on a PC machine, in which the theoretical exponential light curve is adopted to the observations using the least regression method. Using this method, anybody can fit the light curve and get the time scale by moving and clicking themouse.We also used this method to some light curves obtained from the archive and compared our results with those in the literature.

  14. Virtual Testing of Large Composite Structures: A Multiple Length/Time-Scale Framework

    Science.gov (United States)

    Gigliotti, Luigi; Pinho, Silvestre T.

    2015-12-01

    This paper illustrates a multiple length/time-scale framework for the virtual testing of large composite structures. Such framework hinges upon a Mesh Superposition Technique (MST) for the coupling between areas of the structure modelled at different length-scales and upon an efficient solid-to-shell numerical homogenization which exploits the internal symmetries of Unit Cells (UCs). Using this framework, it is possible to minimize the areas of the structure modelled at the lowest- (and computationally demanding) scales and the computational cost required to calculate the homogenised to be used in the higher-scales subdomains of multiscale FE models, as well as to simulate the mechanical response of different parts of the structure using different solvers, depending on where they are expected to provide the most computationally efficient solution. The relevance and key-aspects of the multiple length/time-scale framework are demonstrated through the analysis of a real-sized aeronautical composite component.

  15. Small-time scale network traffic prediction based on a local support vector machine regression model

    Institute of Scientific and Technical Information of China (English)

    Meng Qing-Fang; Chen Yue-Hui; Peng Yu-Hua

    2009-01-01

    In this paper we apply the nonlinear time series analysis method to small-time scale traffic measurement data. The prediction-based method is used to determine the embedding dimension of the traffic data. Based on the reconstructed phase space, the local support vector machine prediction method is used to predict the traffic measurement data, and the BIC-based neighbouring point selection method is used to choose the number of the nearest neighbouring points for the local support vector machine regression model. The experimental results show that the local support vector machine prediction method whose neighbouring points are optimized can effectively predict the small-time scale traffic measurement data and can reproduce the statistical features of real traffic measurements.

  16. Forced bursting and transition mechanism in CO oxidation with three time scales

    Institute of Scientific and Technical Information of China (English)

    Li Xiang-Hong; Bi Qin-Sheng

    2013-01-01

    The mathematical model of CO oxidation with three time scales on platinum group metals is investigated,in which order gaps between the time scales related to external perturbation and the rates associated with different chemical reaction steps exist.Forced bursters,such as point-point type forced bursting and point-cycle type forced bursting,are presented.The bifurcation mechanism of forced bursting is novel,and the phenomenon where two different kinds of spiking states coexist in point-cycle type forced bursting has not been reported in previous work.A double-parameter bifurcation set of the fast subsystem is explored to reveal the transition mechanisms of different forced bursters with parameter variation.

  17. Modelling solar irradiance variability on time scales from minutes to months

    CERN Document Server

    Seleznyov, Andrey D; Krivova, Natalie A; 10.1051/0004-6361/200811138

    2013-01-01

    We analyze and model total solar irradiance variability on time scales from minutes to months, excluding variations due to p-mode oscillations, using a combination of convective and magnetic components. These include granulation, the magnetic network, faculae and sunspots. Analysis of VIRGO data shows that on periods of a day or longer solar variability depends on magnetic activity, but is nearly independent at shorter periods. We assume that only granulation affects the solar irradiance variability on time scales from minutes to hours. Granulation is described as a large sample of bright cells and dark lanes that evolve according to rules deduced from observations and radiation hydrodynamic simulations. Comparison of this model combined with a high time resolution magnetic-field based irradiance reconstruction, with solar data reveals a good correspondence except at periods of 10 to 30 hours. This suggests that the model is missing some power at these periods, which may be due to the absence of supergranulat...

  18. Characteristic time scales of mixing, mass transfer and biomass growth in a Taylor vortex algal photobioreactor.

    Science.gov (United States)

    Gao, Xi; Kong, Bo; Vigil, R Dennis

    2015-12-01

    Recently it has been demonstrated that algal biomass yield can be enhanced using fluid flow patterns known as Taylor vortices. It has been suggested that these growth rate improvements can be attributed to improved light delivery as a result of rapid transport of microorganisms between light and dark regions of the reactor. However, Taylor vortices also strongly impact fluid mixing and interphase (gas-liquid) mass transport, and these in turn may also explain improvements in biomass productivity. To identify the growth-limiting factor in a Taylor vortex algal photobioreactor, experiments were performed to determine characteristic time scales for mixing and mass transfer. By comparing these results with the characteristic time scale for biomass growth, it is shown that algal growth rate in Taylor vortex reactors is not limited by fluid mixing or interphase mass transfer, and therefore the observed biomass productivity improvements are likely attributable to improved light utilization efficiency.

  19. Time-scale effects on the gain-loss asymmetry in stock indices

    Science.gov (United States)

    Sándor, Bulcsú; Simonsen, Ingve; Nagy, Bálint Zsolt; Néda, Zoltán

    2016-08-01

    The gain-loss asymmetry, observed in the inverse statistics of stock indices is present for logarithmic return levels that are over 2 % , and it is the result of the non-Pearson-type autocorrelations in the index. These non-Pearson-type correlations can be viewed also as functionally dependent daily volatilities, extending for a finite time interval. A generalized time-window shuffling method is used to show the existence of such autocorrelations. Their characteristic time scale proves to be smaller (less than 25 trading days) than what was previously believed. It is also found that this characteristic time scale has decreased with the appearance of program trading in the stock market transactions. Connections with the leverage effect are also established.

  20. Climatic changes on millennial time scales--Evidence from a high-resolution loess record

    Institute of Scientific and Technical Information of China (English)

    任剑璋; 丁仲礼; 刘东生; 孙继敏; 周晓权

    1996-01-01

    Studies on a high resolution loess section in Huining County reveal that the behavior of climate shows high instability during the last glaciation. Results reflect that climate in Loess Plateau oscillates on millennial time scales during the last glacial period. These can be teleconnected with the records of Dansgaard-Oeschger cycles and Heinrich events in high latitudes. Results also demonstrate that variations in the intensity of wind regime on the Loess Plateau have a close correlation with the changes of global ice sheets volume. All these suggest that two-level fordngs may drive climate changes in central Asia. The first level is the volume changes of ice sheets and the second level with short time scales is superimposed upon the first level on a nearly global scale.

  1. Time-Scale and Noise Optimality in Self-Organized Critical Adaptive Networks

    CERN Document Server

    Kuehn, Christian

    2011-01-01

    Recent studies have shown that adaptive networks driven by simple local rules can organize into "critical" global steady states, thereby providing another framework for self-organized criticality (SOC). Here we study SOC in an adaptive network considered first by Bornholdt and Rohlf [PRL, 84(26), p.6114-6117, 2000]. We focus on the important convergence to criticality and discover time-scale and noise optimal behaviour as well as a noise-induced phase transition. Due to the complexity of adaptive networks dynamics we suggest to investigate each effect separately by developing simple models. These models reveal three generically possible low-dimensional dynamical behaviors: time-scale resonance (TR), a simplified version of stochastic resonance - which call steady state stochastic resonance (SSR) - as well as noise-induced phase transitions. Thereby, our study not only opens up new directions for optimality in SOC but also applies to a much wider class of dynamical systems.

  2. AN IMPROVED ALGORITHM OF GMM VOICE CONVERSION SYSTEM BASED ON CHANGING THE TIME-SCALE

    Institute of Scientific and Technical Information of China (English)

    Zhou Ying; Zhang Linghua

    2011-01-01

    This paper improves and presents an advanced method of the voice conversion system based on Gaussian Mixture Models (GMM) models by changing the time-scale of speech.The Speech Transformation and Representation using Adaptive Interpolation of weiGHTed spectrum (STRAIGHT) model is adopted to extract the spectrum features,and the GMM models are trained to generate the conversion function.The spectrum features of a source speech will be converted by the conversion function.The time-scale of speech is changed by extracting the converted features and adding to the spectrum.The conversion voice was evaluated by subjective and objective measurements.The results confirm that the transformed speech not only approximates the characteristics of the target speaker,but also more natural and more intelligible.

  3. Selection of outputs for distributed parameter systems by identifiability analysis in the time-scale domain

    Science.gov (United States)

    Teergele, Jane; Danai, Kourosh

    2015-12-01

    A method of sensor location selection is introduced for distributed parameter systems. In this method, the sensitivities of spatial outputs to model parameters are computed by a model and transformed via continuous wavelet transforms into the time-scale domain to characterise the shape attributes of output sensitivities and accentuate their differences. Regions are then sought in the time-scale plane wherein the wavelet coefficient of an output sensitivity surpasses all the others' as indication of the output sensitivity's distinctness. This yields a comprehensive account of identifiability each output provides to the model parameters as the basis of output selection. The proposed output selection strategy is demonstrated for a numerical case of pollutant dispersion by advection and diffusion in a two-dimensional area.

  4. Stability analysis of nonlinear systems by multiple time scaling. [using perturbation methods

    Science.gov (United States)

    Morino, L.

    1974-01-01

    The asymptotic solution for the transient analysis of a general nonlinear system in the neighborhood of the stability boundary was obtained by using the multiple-time-scaling asymptotic-expansion method. The nonlinearities are assumed to be of algebraic nature. Terms of order epsilon to the 3rd power (where epsilon is the order of amplitude of the unknown) are included in the solution. The solution indicates that there is always a limit cycle which is stable (unstable) and exists above (below) the stability boundary if the nonlinear terms are stabilizing (destabilizing). Extension of the solution to include fifth order nonlinear terms is also presented. Comparisons with harmonic balance and with multiple-time-scaling solution of panel flutter equations are also included.

  5. Multiple dynamical time-scales in networks with hierarchically nested modular organization

    Indian Academy of Sciences (India)

    Sitabhra Sinha; Swarup Poria

    2011-11-01

    Many natural and engineered complex networks have intricate mesoscopic organization, e.g., the clustering of the constituent nodes into several communities or modules. Often, such modularity is manifested at several different hierarchical levels, where the clusters defined at one level appear as elementary entities at the next higher level. Using a simple model of a hierarchical modular network, we show that such a topological structure gives rise to characteristic time-scale separation between dynamics occurring at different levels of the hierarchy. This generalizes our earlier result for simple modular networks, where fast intramodular and slow intermodular processes were clearly distinguished. Investigating the process of synchronization of oscillators in a hierarchical modular network, we show the existence of as many distinct time-scales as there are hierarchical levels in the system. This suggests a possible functional role of such mesoscopic organization principle in natural systems, viz., in the dynamical separation of events occurring at different spatial scales.

  6. Time-scale effects on the gain-loss asymmetry in stock indices

    CERN Document Server

    Sándor, Bulcsú

    2016-01-01

    The gain-loss asymmetry, observed in the inverse statistics of stock indices is present for logarithmic return levels that are over $2\\%$, and it is the result of the non-Pearson type auto-correlations in the index. These non-Pearson type correlations can be viewed also as functionally dependent daily volatilities, extending for a finite time interval. A generalized time-window shuffling method is used to show the existence of such auto-correlations. Their characteristic time-scale proves to be smaller (less than $25$ trading days) than what was previously believed. It is also found that this characteristic time-scale has decreased with the appearance of program trading in the stock market transactions. Connections with the leverage effect are also established.

  7. Factors Controlling Variability in the Oxidative Capacity of the Troposphere on Interannual to Interglacial Time Scales

    OpenAIRE

    Murray, Lee Thomas

    2013-01-01

    This thesis explores the natural forces controlling variability of the tropospheric oxidants on interannual to glacial-interglacial time scales. The oxidants (primarily OH and ozone) determine the lifetime of many trace gases of human interest, including air pollutants and long-lived greenhouse gases such as methane. The oxidants respond to meteorological conditions, precursor emissions (natural and anthropogenic), and surface and overhead stratospheric boundary conditions, all of which have ...

  8. Diffusion Time-Scale Invariance, Markovization Processes and Memory Effects in Lennard-Jones Liquids

    OpenAIRE

    Yulmetyev, Renat M.; Mokshin, Anatolii V.; Hänggi, Peter

    2004-01-01

    We report the results of calculation of diffusion coefficients for Lennard-Jones liquids, based on the idea of time-scale invariance of relaxation processes in liquids. The results were compared with the molecular dynamics data for Lennard-Jones system and a good agreement of our theory with these data over a wide range of densities and temperatures was obtained. By calculations of the non-Markovity parameter we have estimated numerically statistical memory effects of diffusion in detail.

  9. Eleven-year cyclicity of the sun on the 2000-year time scale

    Science.gov (United States)

    Nagovitsyn, Yu. A.; Georgieva, K.; Osipova, A. A.; Kuleshova, A. I.

    2015-12-01

    The paper describes the first reconstruction of the annual values of the total area and magnetic flux of sunspots, as well as the Wolf index, on the 2000-year time scale. The procedure was performed by combining data on solar cyclicity amplitudes obtained from a decadal reconstruction (Usoskin et al., 2014) and data on its phases acquired from the numbers of auroras, which is contained in catalogs by Křivský and Silverman.

  10. A time-scale analysis of systematic risk: wavelet-based approach

    OpenAIRE

    Khalfaoui Rabeh, K; Boutahar Mohamed, B

    2011-01-01

    The paper studies the impact of different time-scales on the market risk of individual stock market returns and of a given portfolio in Paris Stock Market by applying the wavelet analysis. To investigate the scaling properties of stock market returns and the lead/lag relationship between them at different scales, wavelet variance and crosscorrelations analyses are used. According to wavelet variance, stock returns exhibit long memory dynamics. The wavelet cross-correlation analysis shows that...

  11. New Insights on the Variability of Ecosystem Functioning Across Time Scales

    Science.gov (United States)

    Pappas, C.; Mahecha, M. D.; Frank, D. C.; Koutsoyiannis, D.

    2015-12-01

    Ecosystem functioning is monitored worldwide over several decades. However, a comparative in-depth characterization of the temporal variability of essential ecosystem processes, such as for example carbon assimilation and respiration is still lacking. The intra-annual (sub-diurnal, diurnal, and seasonal) variability of these processes can be well described by basic mechanisms such as the plant response to light. In contrast, the inter-annual variability and its origins and magnitude, remain highly uncertain. To date, there have only been a few attempts to investigate these issues across sites, ecosystems variables, and time scales, yet a general and comprehensive overview is outstanding. Here, we present a synthesis of a wide range of observations over Europe, namely: (i) eddy covariance measurements of carbon, energy, and water fluxes, (ii) satellite data of leaf area index and photosynthetically active radiation absorbed by plants, (iii) tree-ring widths, and (iv) dendrometer measurements of tree stem radius changes, and we analyze their variability from the half-hourly to the decadal time scale. Our analysis shows that all ecosystems can be characterized by three distinct regimes of variability (sub-daily, daily-seasonal, and seasonal-annual) confined within the ranges of the available resources, i.e., water (precipitation) and energy (radiation and temperature). We find a convergence of the range of variability of hydrometeorological drivers. Surprisingly, such convergence is not reflected in the variability of the ecosystem responses across sites. Although the magnitude of variability of ecosystem functioning varies across sites, the temporal dependences present the same characteristics over time scales spanning five orders of magnitude. We show that this behaviour can be well simulated by combining simple stochastic models with deterministic harmonics (diurnal and annual cycles). This allows us to statistically characterize the short- and long

  12. Existence of Positive Solutions for Higher Order Boundary Value Problem on Time Scales

    Institute of Scientific and Technical Information of China (English)

    XIE DA-PENG; LIU YANG; SUN MING-ZHE; Li Yong

    2013-01-01

    In this paper,we investigate the existence of positive solutions of a class higher order boundary value problems on time scales.The class of boundary value problems educes a four-point (or three-point or two-point) boundary value problems,for which some similar results are established.Our approach relies on the Krasnosel'skii fixed point theorem.The result of this paper is new and extends previously known results.

  13. A typology of time-scale mismatches and behavioral interventions to diagnose and solve conservation problems

    Science.gov (United States)

    Wilson, Robyn S.; Hardisty, David J.; Epanchin-Niell, Rebecca S.; Runge, Michael C.; Cottingham, Kathryn L.; Urban, Dean L.; Maguire, Lynn A.; Hastings, Alan; Mumby, Peter J.; Peters, Debra P. C.

    2016-01-01

    Ecological systems often operate on time scales significantly longer or shorter than the time scales typical of human decision making, which causes substantial difficulty for conservation and management in socioecological systems. For example, invasive species may move faster than humans can diagnose problems and initiate solutions, and climate systems may exhibit long-term inertia and short-term fluctuations that obscure learning about the efficacy of management efforts in many ecological systems. We adopted a management-decision framework that distinguishes decision makers within public institutions from individual actors within the social system, calls attention to the ways socioecological systems respond to decision makers’ actions, and notes institutional learning that accrues from observing these responses. We used this framework, along with insights from bedeviling conservation problems, to create a typology that identifies problematic time-scale mismatches occurring between individual decision makers in public institutions and between individual actors in the social or ecological system. We also considered solutions that involve modifying human perception and behavior at the individual level as a means of resolving these problematic mismatches. The potential solutions are derived from the behavioral economics and psychology literature on temporal challenges in decision making, such as the human tendency to discount future outcomes at irrationally high rates. These solutions range from framing environmental decisions to enhance the salience of long-term consequences, to using structured decision processes that make time scales of actions and consequences more explicit, to structural solutions aimed at altering the consequences of short-sighted behavior to make it less appealing. Additional application of these tools and long-term evaluation measures that assess not just behavioral changes but also associated changes in ecological systems are needed.

  14. Mixed-mode oscillations in a multiple time scale phantom bursting system

    OpenAIRE

    Krupa, Maciej; Vidal, Alexandre; Desroches, Mathieu; Clément, Frédérique

    2012-01-01

    38 pages, 16 figures. International audience In this work we study mixed mode oscillations in a model of secretion of GnRH (Gonadotropin Releasing Hormone). The model is a phantom burster consisting of two feedforward coupled FitzHugh-Nagumo systems, with three time scales. The forcing system (Regulator) evolves on the slowest scale and acts by moving the slow nullcline of the forced system (Secretor). There are three modes of dynamics: pulsatility (transient relaxation oscillation), su...

  15. A General Delta-Nabla Calculus of Variations on Time Scales with Application to Economics

    OpenAIRE

    Dryl, Monika; Torres, Delfim F. M.

    2014-01-01

    We consider a general problem of the calculus of variations on time scales with a cost functional that is the composition of a certain scalar function with delta and nabla integrals of a vector valued field. Euler-Lagrange delta-nabla differential equations are proved, which lead to important insights in the process of discretization. Application of the obtained results to a firm that wants to program its production and investment policies to reach a given production rate and to maximize its ...

  16. Building a minimum frustration framework for brain functions over long time scales.

    Science.gov (United States)

    Tozzi, Arturo; Flå, Tor; Peters, James F

    2016-08-01

    The minimum frustration principle (MFP) is a computational approach stating that, over the long time scales of evolution, proteins' free energy decreases more than expected by thermodynamical constraints as their amino acids assume conformations progressively closer to the lowest energetic state. This Review shows that this general principle, borrowed from protein folding dynamics, can also be fruitfully applied to nervous function. Highlighting the foremost role of energetic requirements, macromolecular dynamics, and above all intertwined time scales in brain activity, the MFP elucidates a wide range of mental processes from sensations to memory retrieval. Brain functions are compared with trajectories that, over long nervous time scales, are attracted toward the low-energy bottom of funnel-like structures characterized by both robustness and plasticity. We discuss how the principle, derived explicitly from evolution and selection of a funneling structure from microdynamics of contacts, is unlike other brain models equipped with energy landscapes, such as the Bayesian and free energy principles and the Hopfield networks. In summary, we make available a novel approach to brain function cast in a biologically informed fashion, with the potential to be operationalized and assessed empirically. © 2016 Wiley Periodicals, Inc. PMID:27114266

  17. Measuring collective behaviour of multicellular ensembles: role of space–time scales

    Indian Academy of Sciences (India)

    S Rajesh; Somdatta Sinha

    2008-06-01

    Living systems are spectacular examples of spatiotemporally organized structures. During the development of complex organization there is dynamic equilibrium between the local and global processes acting at the intra- and intercellular levels in multiple space and time scales. Although in modelling studies such spatiotemporal systems can be described by different space–time scales and at many organizational levels, the experimental quantities measured and predictions useful for practical applications are at a macroscopic (coarser or averaged) level/scale; these are limited by the resolution of the measuring method and experimental protocol. In this work, we address whether the spatiotemporal collective dynamics exhibited by a multiscale system can discriminate between, or be borne out by, the coarse-grained and averaged measurements done at different spatial and temporal scales. Using a simple model of a ring of cells, we show that measurements of both spatial and spatiotemporal average behaviour in this multicellular ensemble can mask the variety of collective dynamics observed at other space–time scales, and exhibit completely different behaviours. Such outcomes of measurements can lead to incomplete and incorrect understanding of physiological functions and pathogenesis in multicell ensembles.

  18. Effects of moonlight on the capturability of frugivorous phyllostomid bats (Chiroptera: Phyllostomidae at different time scales

    Directory of Open Access Journals (Sweden)

    Marco A. R. Mello

    2013-08-01

    Full Text Available Some bat species seem to be lunar phobic, i.e., they avoid flying in bright areas or during bright periods of the night; however, the evidence is still controversial. We think that part of this controversy comes from pooling data on bat captures and moonlight intensity according to broad categories, such as moon phases, which conceal the high variability among nights. Therefore, we used detailed, long-term field data on three phyllostomid bat species, in order to test the hypothesis of lunar phobia at two different time scales: 1 among nights, by pooling data of different nights according to moon phases and testing for differences in the distribution of captures; and 2 within a night, by analyzing the relationship between capturability and moonlight intensity (measured as illuminance in one-hour intervals for 29 individual nights. Although most captures of the studied bat species occurred in the first half of the night, their activity pattern varied largely among nights, and was not always unimodal as commonly assumed. At the larger time scale, all studied bat species showed evidence of lunar phobia, as they were more frequently captured on dark moon phases. Nevertheless, at the smaller time scale, only Carollia perspicillata (Linnaeus, 1758 was less frequently captured on brighter periods of the night. We propose that the unimodal activity pattern assumed for frugivorous phyllostomid bats may be an artifact of data organization, and that activity and lunar phobia are much more variable than previously assumed.

  19. Glottal closure instant and voice source analysis using time-scale lines of maximum amplitude

    Indian Academy of Sciences (India)

    Christophe D’Alessandro; Nicolas Sturmel

    2011-10-01

    1Time-scale representation of voiced speech is applied to voice quality analysis, by introducing the Line of Maximum Amplitude (LoMA) method. This representation takes advantage of the tree patterns observed for voiced speech periods in the time-scale domain. For each period, the optimal LoMA is computed by linking amplitude maxima at each scale of a wavelet transform, using a dynamic programming algorithm. A time-scale analysis of the linear acoustic model of speech production shows several interesting properties. The LoMA points to the glottal closure instants. The LoMA phase delay is linked to the voice open quotient. The cumulated amplitude along the LoMA is related to voicing amplitude. The LoMA spectral centre of gravity is an indication of voice spectral tilt. Following these theoretical considerations, experimental results are reported. Comparative evaluation demonstrates that the LoMA is an effective method for the detection of Glottal Closure Instants (GCI). The effectiveness of LoMA analysis for open quotient, amplitude and spectral tilt estimations is also discussed with the help of some examples.

  20. A biologically plausible model of time-scale invariant interval timing.

    Science.gov (United States)

    Almeida, Rita; Ledberg, Anders

    2010-02-01

    The temporal durations between events often exert a strong influence over behavior. The details of this influence have been extensively characterized in behavioral experiments in different animal species. A remarkable feature of the data collected in these experiments is that they are often time-scale invariant. This means that response measurements obtained under intervals of different durations coincide when plotted as functions of relative time. Here we describe a biologically plausible model of an interval timing device and show that it is consistent with time-scale invariant behavior over a substantial range of interval durations. The model consists of a set of bistable units that switch from one state to the other at random times. We first use an abstract formulation of the model to derive exact expressions for some key quantities and to demonstrate time-scale invariance for any range of interval durations. We then show how the model could be implemented in the nervous system through a generic and biologically plausible mechanism. In particular, we show that any system that can display noise-driven transitions from one stable state to another can be used to implement the timing device. Our work demonstrates that a biologically plausible model can qualitatively account for a large body of data and thus provides a link between the biology and behavior of interval timing.

  1. Multiband optical-NIR variability of blazars on diverse time-scales

    Science.gov (United States)

    Agarwal, Aditi; Gupta, Alok C.; Bachev, R.; Strigachev, A.; Semkov, E.; Wiita, Paul J.; Böttcher, M.; Boeva, S.; Gaur, H.; Gu, M. F.; Peneva, S.; Ibryamov, S.; Pandey, U. S.

    2015-08-01

    To search for optical variability on a wide range of time-scales, we have carried out photometric monitoring of two flat spectrum radio quasars, 3C 454.3 and 3C 279, plus one BL Lac, S5 0716+714, all of which have been exhibiting remarkably high activity and pronounced variability at all wavelengths. CCD magnitudes in B, V, R, and I passbands were determined for ˜7000 new optical observations from 114 nights made during 2011-2014, with an average length of ˜4 h each, at seven optical telescopes: four in Bulgaria, one in Greece, and two in India. We measured multiband optical flux and colour variations on diverse time-scales. Discrete correlation functions were computed among B, V, R, and I observations, to search for any time delays. We found weak correlations in some cases with no significant time lags. The structure function method was used to estimate any characteristic time-scales of variability. We also investigated the spectral energy distribution of the three blazars using B, V, R, I, J, and K passband data. We found that the sources almost always follow a bluer-when-brighter trend. We discuss possible physical causes of the observed spectral variability.

  2. Time Scale Creator - A Visualization and Database Tool for Earth History

    Science.gov (United States)

    Huang, A.; Ogg, J.

    2008-12-01

    Unravelling Earth's history requires the ability to compare biologic, lithologic, chemical, magnetic and other records from different regions. Published correlation charts provide some details, but tend to be unwieldy, difficult to update, and awkward to merge with other records. The Time Scale Creator program of the International Commission on Stratigraphy provides a suite of global and regional reference datasets (approximately 20,000 Phanerozoic datums, plus geochemical and other trends) within a visualization package. Users can append additional regional lithostratigraphic or other datasets, then create on-screen charts for any portion of the geologic time scale with any subsets of the extensive stratigraphic data. In addition to scalable-vector graphics (SVG) or PDF file output, the on-screen display contains "hot-cursor- points" which open up windows with additional information on events, zones, and URL links to external documentation. For example, a user can select from within a datapack with 50 representative stratigraphic columns spanning the British Isles, then display lithologic sections against models of global sea-level trends or adjacent to Sub-boreal ammonite zones, and the pop-up window for each formation is linked directly to the British Geologic Survey lexicon entry. Much in the way that GIS greatly enhances accessibility to spatial data, the Time Scale Creator and its temporal data are completely digital, allowing quick and easy distribution and updating. The database and visualization package are a convenient reference tool, chart-production device, and educational program.

  3. Active open boundary forcing using dual relaxation time-scales in downscaled ocean models

    Science.gov (United States)

    Herzfeld, M.; Gillibrand, P. A.

    2015-05-01

    Regional models actively forced with data from larger scale models at their open boundaries often contain motion at different time-scales (e.g. tidal and low frequency). These motions are not always individually well specified in the forcing data, and one may require a more active boundary forcing while the other exert less influence on the model interior. If a single relaxation time-scale is used to relax toward these data in the boundary equation, then this may be difficult. The method of fractional steps is used to introduce dual relaxation time-scales in an open boundary local flux adjustment scheme. This allows tidal and low frequency oscillations to be relaxed independently, resulting in a better overall solution than if a single relaxation parameter is optimized for tidal (short relaxation) or low frequency (long relaxation) boundary forcing. The dual method is compared to the single relaxation method for an idealized test case where a tidal signal is superimposed on a steady state low frequency solution, and a real application where the low frequency boundary forcing component is derived from a global circulation model for a region extending over the whole Great Barrier Reef, and a tidal signal subsequently superimposed.

  4. A Black Hole Mass-Variability Time Scale Correlation at Submillimeter Wavelengths

    CERN Document Server

    Bower, Geoffrey C; Markoff, Sera; Gurwell, Mark A; Rao, Ramprasad; McHardy, Ian

    2015-01-01

    We analyze the light curves of 413 radio sources at submillimeter wavelengths using data from the Submillimeter Array calibrator database. The database includes more than 20,000 observations at 1.3 and 0.8 mm that span 13 years. We model the light curves as a damped random walk and determine a characteristic time scale $\\tau$ at which the variability amplitude saturates. For the vast majority of sources, primarily blazars and BL Lac objects, we find only lower limits on $\\tau$. For two nearby low luminosity active galactic nuclei, M81 and M87, however, we measure $\\tau=1.6^{+3.0}_{-0.9}$ days and $\\tau=45^{+61}_{-24}$ days, respectively ($2\\sigma$ errors). Including the previously measured $\\tau=0.33\\pm 0.16$ days for Sgr A*, we show an approximately linear correlation between $\\tau$ and black hole mass for these nearby LLAGN. Other LLAGN with spectra that peak in the submm are expected to follow this correlation. These characteristic time scales are comparable to the minimum time scale for emission processes...

  5. A cardioactive peptide from the southern armyworm, Spodoptera eridania.

    Science.gov (United States)

    Furuya, K; Hackett, M; Cirelli, M A; Schegg, K M; Wang, H; Shabanowitz, J; Hunt, D F; Schooley, D A

    1999-01-01

    A cardioactive peptide was isolated from extracts of whole heads of the southern armyworm, Spodoptera eridania. This peptide has the sequence ENFAVGCTPGYQRTADGRCKPTF (Mr = 2516.8), determined from both Edman sequencing and tandem mass spectrometry in combination with off-line micropreparative capillary liquid chromatography. This peptide, termed Spoer-CAP23, has excitatory effects on a semi-isolated heart from larval Manduca sexta, causing an inotropic effect at low concentrations of peptide and chronotropic and inotropic effects at high doses. The threshold concentration for stimulatory effects of the synthetic peptide on the semi-isolated heart was about 1 nM, suggesting a physiological role as a neuropeptide. PMID:10098624

  6. Earth History databases and visualization - the TimeScale Creator system

    Science.gov (United States)

    Ogg, James; Lugowski, Adam; Gradstein, Felix

    2010-05-01

    The "TimeScale Creator" team (www.tscreator.org) and the Subcommission on Stratigraphic Information (stratigraphy.science.purdue.edu) of the International Commission on Stratigraphy (www.stratigraphy.org) has worked with numerous geoscientists and geological surveys to prepare reference datasets for global and regional stratigraphy. All events are currently calibrated to Geologic Time Scale 2004 (Gradstein et al., 2004, Cambridge Univ. Press) and Concise Geologic Time Scale (Ogg et al., 2008, Cambridge Univ. Press); but the array of intercalibrations enable dynamic adjustment to future numerical age scales and interpolation methods. The main "global" database contains over 25,000 events/zones from paleontology, geomagnetics, sea-level and sequence stratigraphy, igneous provinces, bolide impacts, plus several stable isotope curves and image sets. Several regional datasets are provided in conjunction with geological surveys, with numerical ages interpolated using a similar flexible inter-calibration procedure. For example, a joint program with Geoscience Australia has compiled an extensive Australian regional biostratigraphy and a full array of basin lithologic columns with each formation linked to public lexicons of all Proterozoic through Phanerozoic basins - nearly 500 columns of over 9,000 data lines plus hot-curser links to oil-gas reference wells. Other datapacks include New Zealand biostratigraphy and basin transects (ca. 200 columns), Russian biostratigraphy, British Isles regional stratigraphy, Gulf of Mexico biostratigraphy and lithostratigraphy, high-resolution Neogene stable isotope curves and ice-core data, human cultural episodes, and Circum-Arctic stratigraphy sets. The growing library of datasets is designed for viewing and chart-making in the free "TimeScale Creator" JAVA package. This visualization system produces a screen display of the user-selected time-span and the selected columns of geologic time scale information. The user can change the

  7. 反相液相色谱-串联质谱法鉴定油菜蜂花粉中的蛋白质及活性肽%Application of reversed-phase liquid chromatography-tandem mass spectrometry in the identification of protein and bioactivity peptides from rape bee pollen

    Institute of Scientific and Technical Information of China (English)

    郭静; 晏嘉泽; 郭明; 靳艳

    2014-01-01

    Based on the shotgun proteomic method,rape bee pollen protein was prepared with ultrasonic extraction and digested by trypsin,then separated and sequenced by reversed-phase liquid chromatography-tandem mass spectrometry( RPLC-MS / MS),followed by protein data-base searching. After the above analysis,353 peptides were identified and the molecular biolog-ical functions of 239 proteins have been known. The identified molecular biological functions of these proteins mainly included binding activity,enzyme activity,transporter activity,inhibitor activity and so on. Five peptides were obtained after the screening and appropriate amino acid modification among the identified 353 peptides,according to the relationship between the sequence structure of the bioactivity peptide and angiotensin converting enzyme( ACE)inhibi-tor activity. The five peptides were speculated to have ACE inhibitor activity and synthesized to detect ACE inhibitor activity. The results showed that all of the five peptides had good ACE inhibitor activity. The peptides of AELDIVLALF and LAVNLIPFP among the five peptides dis-played especially good ACE inhibition with half maximal inhibitory concentration( IC 50 ) of (10. 65±0. 50)μmol / L and(23. 66±1. 08)μmol / L,respectively. This method is rapid,low-cost and achieves the goal of high-throughput screening of bioactivity peptides that greatly shorten the period of identification compared with traditional methods.%基于鸟枪法蛋白质组学分析方法,使用反相液相色谱-串联质谱(RPLC-MS / MS)系统分析油菜蜂花粉蛋白质的胰蛋白酶酶解产物,结合数据库检索,共鉴定到353条肽段。鉴定到的肽段所归属的蛋白质中有239个蛋白质可检索到其分子生物学功能,主要功能为结合活性、酶活性、运输活性、抑制活性等。根据血管紧张素转化酶( ACE)抑制肽活性与多肽构效之间的关系,从鉴定到的肽段中筛选并适当修饰后得到5

  8. 高效液相色谱法测定多肽AP25的含量及有关物质%Content Determination of Peptide AP25 And Its Related Substances by High Performance Liquid Chromatography

    Institute of Scientific and Technical Information of China (English)

    尼格尔热依·亚迪卡尔; 徐寒梅

    2013-01-01

    建立多肽AP25含量测定及有关物质检查的高效液相色谱方法.采用COSMOSIL C18色谱柱(250 mm×4.6mm,5μm);以0.05 mol/L磷酸二氢钠(用磷酸调pH值至2.5)为流动相A,乙腈为流动相B,进行梯度洗脱;检测波长为220 nm;体积流量为1.0 mL/min;柱温为25℃;进样体积为20μL.在该色谱条件下,各有关物质峰均可与AP25主峰良好分离,理论塔板数不低于5 000,AP25浓度在0.8 ~1.2 mg/mL(r =0.999 6,n=5)和18~42μg/mL(r =0.999 5,n=5)范围内与峰面积呈良好线性关系;检测限和定量限分别为0.5ng和1ng;含量和有关物质检测方法的重复性试验RSD值分别为0.60%和0.48%(n=6),中间精密度试验RSD值分别为0.35%和0.80%(n=12).3批原料药的含量和有关物质检测结果分别为99.56%,99.84%,99.74%和2.17%,0.98%,2.19%.本方法简便易行,灵敏度高,耐用性、专属性良好,准确稳定,可用于测定多肽AP25的含量及有关物质.%To establish a high performance liquid chromatography (HPLC) method for content determination of the peptide AP25 and its related substances,the separation was performed on a COSMOSIL C18 column (250 mm × 4.6mm,5 μm).The mobile phase A consisted of 0.05 mol/L sodium dihydrogen phosphate (adjusting the pH value to 2.5 with the phosphonic acid),the mobile phase B was acetonitrile,the separation process was gradient elution:the ratio of mobile phase B rised from 10% to 40% within 30 minutes.The retention time of AP25 main peak was kept at about 13 minutes.The detection wavelength was at 220 nm and the flow rate was 1.0 mL/min,the column temperature was 25 ℃,and the volume of injection was 20 μL.Under this chromatographic condition,an excellent separation was achieved for AP25 and its related substances,the degree of separability was more than 1.5,and the number of theoretical plates with AP25 was more than 5 000.There was a good linear relationship between the peak area and the concentration of AP25

  9. Atomic Force Microscope Mediated Chromatography

    Science.gov (United States)

    Anderson, Mark S.

    2013-01-01

    The atomic force microscope (AFM) is used to inject a sample, provide shear-driven liquid flow over a functionalized substrate, and detect separated components. This is demonstrated using lipophilic dyes and normal phase chromatography. A significant reduction in both size and separation time scales is achieved with a 25-micron-length column scale, and one-second separation times. The approach has general applications to trace chemical and microfluidic analysis. The AFM is now a common tool for ultra-microscopy and nanotechnology. It has also been demonstrated to provide a number of microfluidic functions necessary for miniaturized chromatography. These include injection of sub-femtoliter samples, fluidic switching, and sheardriven pumping. The AFM probe tip can be used to selectively remove surface layers for subsequent microchemical analysis using infrared and tip-enhanced Raman spectroscopy. With its ability to image individual atoms, the AFM is a remarkably sensitive detector that can be used to detect separated components. These diverse functional components of microfluidic manipulation have been combined in this work to demonstrate AFM mediated chromatography. AFM mediated chromatography uses channel-less, shear-driven pumping. This is demonstrated with a thin, aluminum oxide substrate and a non-polar solvent system to separate a mixture of lipophilic dyes. In conventional chromatographic terms, this is analogous to thin-layer chromatography using normal phase alumina substrate with sheardriven pumping provided by the AFM tip-cantilever mechanism. The AFM detection of separated components is accomplished by exploiting the variation in the localized friction of the separated components. The AFM tip-cantilever provides the mechanism for producing shear-induced flows and rapid pumping. Shear-driven chromatography (SDC) is a relatively new concept that overcomes the speed and miniaturization limitations of conventional liquid chromatography. SDC is based on a

  10. Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography*

    Science.gov (United States)

    Huang, Honggang; Haar Petersen, Martin; Ibañez-Vea, Maria; Lassen, Pernille S.; Larsen, Martin R.; Palmisano, Giuseppe

    2016-01-01

    Cysteine is a rare and conserved amino acid involved in most cellular functions. The thiol group of cysteine can be subjected to diverse oxidative modifications that regulate many physio-pathological states. In the present work, a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) was synthesized to selectively label cysteine-containing peptides (Cys peptides) followed by their enrichment with titanium dioxide (TiO2) and subsequent mass spectrometric analysis. The CysPAT strategy was developed using a synthetic peptide, a standard protein and subsequently the strategy was applied to protein lysates from Hela cells, achieving high specificity and enrichment efficiency. In particular, for Cys proteome analysis, the method led to the identification of 7509 unique Cys peptides from 500 μg of HeLa cell lysate starting material. Furthermore, the method was developed to simultaneously enrich Cys peptides and phosphorylated peptides. This strategy was applied to SILAC labeled Hela cells subjected to 5 min epidermal growth factor (EGF) stimulation. In total, 10440 unique reversibly modified Cys peptides (3855 proteins) and 7339 unique phosphopeptides (2234 proteins) were simultaneously identified from 250 μg starting material. Significant regulation was observed in both phosphorylation and reversible Cys modification of proteins involved in EGFR signaling. Our data indicates that EGF stimulation can activate the well-known phosphorylation of EGFR and downstream signaling molecules, such as mitogen-activated protein kinases (MAPK1 and MAPK3), however, it also leads to substantial modulation of reversible cysteine modifications in numerous proteins. Several protein tyrosine phosphatases (PTPs) showed a reduction of the catalytic Cys site in the conserved putative phosphatase HC(X)5R motif indicating an activation and subsequent de-phosphorylation of proteins involved in the EGF signaling pathway. Overall, the CysPAT strategy is a straight forward, easy and promising

  11. Simultaneous quantification of α-lactalbumin and β-casein in human milk using ultra-performance liquid chromatography with tandem mass spectrometry based on their signature peptides and winged isotope internal standards.

    Science.gov (United States)

    Chen, Qi; Zhang, Jingshun; Ke, Xing; Lai, Shiyun; Li, Duo; Yang, Jinchuan; Mo, Weimin; Ren, Yiping

    2016-09-01

    In recent years, there is an increasing need to measure the concentration of individual proteins in human milk, instead of total human milk proteins. Due to lack of human milk protein standards, there are only few quantification methods established. The objective of the present work was to develop a simple and rapid quantification method for simultaneous determination of α-lactalbumin and β-casein in human milk using signature peptides according to a modified quantitative proteomics strategy. The internal standards containing the signature peptide sequences were synthesized with isotope-labeled amino acids. The purity of synthesized peptides as standards was determined by amino acid analysis method and area normalization method. The contents of α-lactalbumin and β-casein in human milk were measured according to the equimolar relationship between the two proteins and their corresponding signature peptides. The method validation results showed a satisfied linearity (R(2)>0.99) and recoveries (97.2-102.5% for α-lactalbumin and 99.5-100.3% for β-casein). The limit of quantification for α-lactalbumin and β-casein was 8.0mg/100g and 1.2mg/100g, respectively. CVs for α-lactalbumin and β-casein in human milk were 5.2% and 3.0%. The contents of α-lactalbumin and β-casein in 147 human milk samples were successfully determined by the established method and their contents were 205.5-578.2mg/100g and 116.4-467.4mg/100g at different lactation stages. The developed method allows simultaneously determination of α-lactalbumin and β-casein in human milk. The quantitative strategy based on signature peptide should be applicable to other endogenous proteins in breast milk and other body fluids.

  12. Simultaneous quantification of α-lactalbumin and β-casein in human milk using ultra-performance liquid chromatography with tandem mass spectrometry based on their signature peptides and winged isotope internal standards.

    Science.gov (United States)

    Chen, Qi; Zhang, Jingshun; Ke, Xing; Lai, Shiyun; Li, Duo; Yang, Jinchuan; Mo, Weimin; Ren, Yiping

    2016-09-01

    In recent years, there is an increasing need to measure the concentration of individual proteins in human milk, instead of total human milk proteins. Due to lack of human milk protein standards, there are only few quantification methods established. The objective of the present work was to develop a simple and rapid quantification method for simultaneous determination of α-lactalbumin and β-casein in human milk using signature peptides according to a modified quantitative proteomics strategy. The internal standards containing the signature peptide sequences were synthesized with isotope-labeled amino acids. The purity of synthesized peptides as standards was determined by amino acid analysis method and area normalization method. The contents of α-lactalbumin and β-casein in human milk were measured according to the equimolar relationship between the two proteins and their corresponding signature peptides. The method validation results showed a satisfied linearity (R(2)>0.99) and recoveries (97.2-102.5% for α-lactalbumin and 99.5-100.3% for β-casein). The limit of quantification for α-lactalbumin and β-casein was 8.0mg/100g and 1.2mg/100g, respectively. CVs for α-lactalbumin and β-casein in human milk were 5.2% and 3.0%. The contents of α-lactalbumin and β-casein in 147 human milk samples were successfully determined by the established method and their contents were 205.5-578.2mg/100g and 116.4-467.4mg/100g at different lactation stages. The developed method allows simultaneously determination of α-lactalbumin and β-casein in human milk. The quantitative strategy based on signature peptide should be applicable to other endogenous proteins in breast milk and other body fluids. PMID:27295510

  13. Efficient purification of unique antibodies using peptide affinity-matrix columns

    DEFF Research Database (Denmark)

    Jensen, Liselotte Brix; Riise, Erik; Nielsen, Leif Kofoed;

    2004-01-01

    -99. Several peptide epitopes were identified and all of them recognised specifically MK16. One peptide, ER6.1, was selected and linked to beaded agarose and demonstrated excellent performance as a peptide affinity chromatography matrix. This epitope matrix was efficient in the purification of MK16 Fab...

  14. Loschmidt echo in many-spin systems: contrasting time scales of local and global measurements.

    Science.gov (United States)

    Zangara, Pablo R; Bendersky, Denise; Levstein, Patricia R; Pastawski, Horacio M

    2016-06-13

    A local excitation in a quantum many-spin system evolves deterministically. A time-reversal procedure, involving the inversion of the signs of every energy and interaction, should produce the excitation revival. This idea, experimentally coined in nuclear magnetic resonance, embodies the concept of the Loschmidt echo (LE). While such an implementation involves a single spin autocorrelation M(1,1), i.e. a local LE, theoretical efforts have focused on the study of the recovery probability of a complete many-body state, referred to here as global or many-body LE MMB Here, we analyse the relation between these magnitudes, with regard to their characteristic time scales and their dependence on the number of spins N We show that the global LE can be understood, to some extent, as the simultaneous occurrence of N independent local LEs, i.e. MMB∼(M(1,1))(N/4) This extensive hypothesis is exact for very short times and confirmed numerically beyond such a regime. Furthermore, we discuss a general picture of the decay of M1,1 as a consequence of the interplay between the time scale that characterizes the reversible interactions (T(2)) and that of the perturbation (τ(Σ)). Our analysis suggests that the short-time decay, characterized by the time scale τ(Σ), is greatly enhanced by the complex processes that occur beyond T(2) This would ultimately lead to the experimentally observed T(3), which was found to be roughly independent of τ(Σ) but closely tied to T(2). PMID:27140970

  15. Loschmidt echo in many-spin systems: contrasting time scales of local and global measurements.

    Science.gov (United States)

    Zangara, Pablo R; Bendersky, Denise; Levstein, Patricia R; Pastawski, Horacio M

    2016-06-13

    A local excitation in a quantum many-spin system evolves deterministically. A time-reversal procedure, involving the inversion of the signs of every energy and interaction, should produce the excitation revival. This idea, experimentally coined in nuclear magnetic resonance, embodies the concept of the Loschmidt echo (LE). While such an implementation involves a single spin autocorrelation M(1,1), i.e. a local LE, theoretical efforts have focused on the study of the recovery probability of a complete many-body state, referred to here as global or many-body LE MMB Here, we analyse the relation between these magnitudes, with regard to their characteristic time scales and their dependence on the number of spins N We show that the global LE can be understood, to some extent, as the simultaneous occurrence of N independent local LEs, i.e. MMB∼(M(1,1))(N/4) This extensive hypothesis is exact for very short times and confirmed numerically beyond such a regime. Furthermore, we discuss a general picture of the decay of M1,1 as a consequence of the interplay between the time scale that characterizes the reversible interactions (T(2)) and that of the perturbation (τ(Σ)). Our analysis suggests that the short-time decay, characterized by the time scale τ(Σ), is greatly enhanced by the complex processes that occur beyond T(2) This would ultimately lead to the experimentally observed T(3), which was found to be roughly independent of τ(Σ) but closely tied to T(2).

  16. Multiscale Modeling of Human-Water Interactions: The Role of Time-Scales

    Science.gov (United States)

    Bloeschl, G.; Sivapalan, M.

    2015-12-01

    Much of the interest in hydrological modeling in the past decades revolved around resolving spatial variability. With the rapid changes brought about by human impacts on the hydrologic cycle, there is now an increasing need to refocus on time dependency. We present a co-evolutionary view of hydrologic systems, in which every part of the system including human systems, co-evolve, albeit at different rates. The resulting coupled human-nature system is framed as a dynamical system, characterized by interactions of fast and slow time scales and feedbacks between environmental and social processes. This gives rise to emergent phenomena such as the levee effect, adaptation to change and system collapse due to resource depletion. Changing human values play a key role in the emergence of these phenomena and should therefore be considered as internal to the system in a dynamic way. The co-evolutionary approach differs from the traditional view of water resource systems analysis as it allows for path dependence, multiple equilibria, lock-in situations and emergent phenomena. The approach may assist strategic water management for long time scales through facilitating stakeholder participation, exploring the possibility space of alternative futures, and helping to synthesise the observed dynamics of different case studies. Future research opportunities include the study of how changes in human values are connected to human-water interactions, historical analyses of trajectories of system co-evolution in individual places and comparative analyses of contrasting human-water systems in different climate and socio-economic settings. Reference Sivapalan, M. and G. Blöschl (2015) Time Scale Interactions and the Co-evolution of Humans and Water. Water Resour. Res., 51, in press.

  17. The Steppengrille (Gryllus spec./assimilis: selective filters and signal mismatch on two time scales.

    Directory of Open Access Journals (Sweden)

    Matti Michael Rothbart

    Full Text Available In Europe, several species of crickets are available commercially as pet food. Here we investigated the calling song and phonotactic selectivity for sound patterns on the short and long time scales for one such a cricket, Gryllus spec., available as "Gryllus assimilis", the Steppengrille, originally from Ecuador. The calling song consisted of short chirps (2-3 pulses, carrier frequency: 5.0 kHz emitted with a pulse period of 30.2 ms and chirp rate of 0.43 per second. Females exhibited high selectivity on both time scales. The preference for pulse period peaked at 33 ms which was higher then the pulse period produced by males. Two consecutive pulses per chirp at the correct pulse period were already sufficient for positive phonotaxis. The preference for the chirp pattern was limited by selectivity for small chirp duty cycles and for chirp periods between 200 ms and 500 ms. The long chirp period of the songs of males was unattractive to females. On both time scales a mismatch between the song signal of the males and the preference of females was observed. The variability of song parameters as quantified by the coefficient of variation was below 50% for all temporal measures. Hence, there was not a strong indication for directional selection on song parameters by females which could account for the observed mismatch. The divergence of the chirp period and female preference may originate from a founder effect, when the Steppengrille was cultured. Alternatively the mismatch was a result of selection pressures exerted by commercial breeders on low singing activity, to satisfy customers with softly singing crickets. In the latter case the prominent divergence between male song and female preference was the result of domestication and may serve as an example of rapid evolution of song traits in acoustic communication systems.

  18. On the time-scales of magmatism at island-arc volcanoes.

    Science.gov (United States)

    Turner, S P

    2002-12-15

    Precise information on time-scales and rates of change is fundamental to an understanding of natural processes and the development of quantitative physical models in the Earth sciences. U-series isotope studies are revolutionizing this field by providing time information in the range 10(2)-10(4) years, which is similar to that of many modern Earth processes. I review how the application of U-series isotopes has been used to constrain the time-scales of magma formation, ascent and storage beneath island-arc volcanoes. Different elements are distilled-off the subducting plate at different times and in different places. Contributions from subducted sediments to island-arc lava sources appear to occur some 350 kyr to 4 Myr prior to eruption. Fluid release from the subducting oceanic crust into the mantle wedge may be a multi-stage process and occurs over a period ranging from a few hundred kyr to less than one kyr prior to eruption. This implies that dehydration commences prior to the initiation of partial melting within the mantle wedge, which is consistent with recent evidence that the onset of melting is controlled by an isotherm and thus the thermal structure within the wedge. U-Pa disequilibria appear to require a component of decompression melting, possibly due to the development of gravitational instabilities. The preservation of large (226)Ra disequilibria permits only a short period of time between fluid addition and eruption. This requires rapid melt segregation, magma ascent by channelled flow and minimal residence time within the lithosphere. The evolution from basalt to basaltic andesite probably occurs rapidly during ascent or in magma reservoirs inferred from some geophysical data to lie within the lithospheric mantle. The flux across the Moho is broadly andesitic, and some magmas subsequently stall in more shallow crustal-level magma chambers, where they evolve to more differentiated compositions on time-scales of a few thousand years or less.

  19. Gauss-Markov processes as space-time scaled stationary Ornstein-Uhlenbeck processes

    OpenAIRE

    Barczy, Matyas; Kern, Peter

    2014-01-01

    We present a class of Gauss-Markov processes which can be represented as space-time scaled stationary Ornstein-Uhlenbeck processes defined on the real line. To give examples, we study scaled Wiener bridges, Ornstein-Uhlenbeck type bridges, weighted Wiener bridges and so called F-Wiener bridges. By giving counterexamples, we also point out that this kind of representation does not hold in general, e.g., for a zero area Wiener bridge. To give a possible application, we show that our results can...

  20. Transport equation for the time scale of a turbulent scalar field

    International Nuclear Information System (INIS)

    The two-parametric turbulence models cause serious difficulties by modeling the near-wall flows due to absence of the natural boundary condition on the wall for dissipation of the ε turbulence energy and the εθ scalar field destruction. This difficulty may be overcome, if instead of the ε and εθ, as the second parameter of the model, to apply the time scales of the turbulent dynamic and scalar fields. The equation of the scalar field is derived and numerical coefficients included therein, are determined from the simplest problems on the turbulent heat transfer

  1. Chaotic phenomenon and the maximum predictable time scale of observation series of urban hourly water consumption

    Institute of Scientific and Technical Information of China (English)

    柳景青; 张士乔; 俞申凯

    2004-01-01

    The chaotic characteristics and maximum predictable time scale of the observation series of hourly water consumption in Hangzhou were investigated using the advanced algorithm presented here is based on the conventional Wolf's algorithm for the largest Lyapunov exponent. For comparison, the largest Lyapunov exponents of water consumption series with one-hour and 24-hour intervals were calculated respectively. The results indicated that chaotic characteristics obviously exist in the hourly water consumption system; and that observation series with 24-hour interval have longer maximum predictable scale than hourly series. These findings could have significant practical application for better prediction of urban hourly water consumption.

  2. Raman Spectra of Interchanging β lactamase Inhibitor Intermediates on the Millisecond Time Scale

    OpenAIRE

    Torkabadi, Hossein Heidari; Che, Tao; Shou, Jingjing; Shanmugam, Sivaprakash; Crowder, Michael W.; Robert A Bonomo; Pusztai-Carey, Marianne; Carey, Paul R.

    2013-01-01

    Rapid mix - rapid freeze is a powerful method to study the mechanisms of enzyme-substrate reactions in solution. Here we report a protocol that combines this method with normal (non-resonance) Raman microscopy to enable us to define molecular details of intermediates at early time points. With this combined method, SHV-1, a class A β-lactamase, and tazobactam, a commercially available β-lactamase inhibitor, were rapidly mixed on the millisecond time-scale, then were flash-frozen by injecting ...

  3. Oscillation of Third-order Delay Dynamic Equations on Time Scales

    Institute of Scientific and Technical Information of China (English)

    YANG Jia-shan

    2014-01-01

    This paper is concerned with the oscillatory behavior of a class of third-order nonlinear variable delay neutral functional dynamic equations on time scale. By using the generalized Riccati transformation and inequality technique, we establish some new oscilla-tion criteria for the equations. Our results extend and improve some known results, but also unify the oscillation of third-order nonlinear variable delay functional differential equations and functional difference equations with a nonlinear neutral term. Some examples are given to illustrate the importance of our results.

  4. Martensitic phase transition in UNi{sub 2}Sn measured in two time scales

    Energy Technology Data Exchange (ETDEWEB)

    Drost, A. [Netherlands Energy Research Foundation (ECN), Petten (Netherlands); Mulder, F.M. [Rijksuniversiteit Leiden (Netherlands). Kamerlingh Onnes Lab.; Thiel, R.C. [Rijksuniversiteit Leiden (Netherlands). Kamerlingh Onnes Lab.; Frikkee, E. [Netherlands Energy Research Foundation (ECN), Petten (Netherlands); Nieuwenhuys, G.J. [Rijksuniversiteit Leiden (Netherlands). Kamerlingh Onnes Lab.

    1994-08-01

    The martensitic phase transition in the Heusler compound UNi{sub 2}Sn has been investigated by means of neutron powder diffraction and {sup 119}Sn Moessbauer spectroscopy. It is shown that the low-temperature orthorhombic structure is gradually transformed into the high-temperature cubic structure between 200 and 330 K. Although the Moessbauer spectra for these two phases are virtually identical, an extraordinary line broadening is observed in the transition region. The broadening is explained in terms of the thermal excitation of domain walls, moving on the Moessbauer time scale. In the domain walls the atoms hop from cubic to orthorhombic position or vice versa. (orig.).

  5. Rotational relaxation time as unifying time scale for polymer and fiber drag reduction

    CERN Document Server

    Boelens, A M P

    2015-01-01

    Using hybrid Direct Numerical Simulation with Langevin dynamics, a comparison is performed between polymer and fiber stress tensors in turbulent flow. The stress tensors are found to be similar, suggesting a common drag reducing mechanism in the onset regime for both flexible polymers and rigid fibers. Since fibers do not have an elastic backbone this must be a viscous effect. Analysis of the viscosity tensor reveals that all terms are negligible, except the off-diagonal shear viscosity associated with rotation. Based on this analysis, we identify the rotational orientation time as the unifying time scale setting a new time criterion for drag reduction by both flexible polymers and rigid fibers.

  6. Kondo time scales for quantum dots: Response to pulsed bias potentials

    International Nuclear Information System (INIS)

    The response of a quantum dot in the Kondo regime to rectangular pulsed bias potentials of various strengths and durations is studied theoretically. It is found that the rise time is faster than the fall time, and also faster than time scales normally associated with the Kondo problem. For larger values of the pulsed bias, one can induce dramatic oscillations in the induced current with a frequency approximating the splitting between the Kondo peaks that would be present in steady state. The effect persists in the total charge transported per pulse, which should facilitate the experimental observation of the phenomenon. (c) 2000 The American Physical Society

  7. Tropical Pacific/Atlantic Ocean interactions at multi‐decadal time scales

    OpenAIRE

    Latif, Mojib

    2001-01-01

    Analysis of sea surface temperature (SST) observations suggests a pan‐oceanic interaction between the tropical Pacific and the Atlantic Ocean at multi‐decadal time scales, such that periods of anomalously high SSTs in the tropical Pacific are followed by a basin‐wide SST dipole in the Atlantic Ocean with a time delay of a few decades. The SST anomaly structure in the Atlantic Ocean is reminscent of variations in the North Atlantic thermohaline circulation. The two ocean basins are linked thro...

  8. Separation of N-derivatized di- and tri-peptide stereoisomers by micro-liquid chromatography using a quinidine-based monolithic column - Analysis of l-carnosine in dietary supplements.

    Science.gov (United States)

    Wang, Qiqin; Sánchez-López, Elena; Han, Hai; Wu, Huihui; Zhu, Peijie; Crommen, Jacques; Marina, Maria Luisa; Jiang, Zhengjin

    2016-01-01

    In the present study, a new analytical methodology was developed enabling the enantiomeric determination of N-derivatized di- and tri-peptides in dietary supplements using chiral micro-LC on a monolithic column consisting of poly(O-9-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-co-2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) (poly(MQD-co-HEMA-co-EDMA)). After optimization of the mobile phase conditions, a baseline resolution of the stereoisomers of 24 out of 53 N-derivatized di- and tri-peptides was obtained. 3,5-Dinitrobenzoyl- and 3,5-dichlorobenzoyl-peptide stereoisomers were separated with exceptionally high selectivity and resolution. The monolithic column was then applied to the quantitative analysis of l-carnosine and its enantiomeric impurity in three different commercial dietary supplements. Method validation demonstrated satisfactory results in terms of linearity, precision, selectivity, accuracy and limits of detection and quantification. The determined amounts of l-carnosine in commercial formulations were in agreement with the labeled content for all analyzed samples, and the enantiomeric impurity was found to be below the limit of detection (LOD), showing the potential of the poly(MQD-co-HEMA-co-EDMA) monolithic column as a reliable tool for the quality control of l-carnosine in dietary supplements by micro-LC.

  9. Separation of N-derivatized di- and tri-peptide stereoisomers by micro-liquid chromatography using a quinidine-based monolithic column - Analysis of l-carnosine in dietary supplements.

    Science.gov (United States)

    Wang, Qiqin; Sánchez-López, Elena; Han, Hai; Wu, Huihui; Zhu, Peijie; Crommen, Jacques; Marina, Maria Luisa; Jiang, Zhengjin

    2016-01-01

    In the present study, a new analytical methodology was developed enabling the enantiomeric determination of N-derivatized di- and tri-peptides in dietary supplements using chiral micro-LC on a monolithic column consisting of poly(O-9-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-co-2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) (poly(MQD-co-HEMA-co-EDMA)). After optimization of the mobile phase conditions, a baseline resolution of the stereoisomers of 24 out of 53 N-derivatized di- and tri-peptides was obtained. 3,5-Dinitrobenzoyl- and 3,5-dichlorobenzoyl-peptide stereoisomers were separated with exceptionally high selectivity and resolution. The monolithic column was then applied to the quantitative analysis of l-carnosine and its enantiomeric impurity in three different commercial dietary supplements. Method validation demonstrated satisfactory results in terms of linearity, precision, selectivity, accuracy and limits of detection and quantification. The determined amounts of l-carnosine in commercial formulations were in agreement with the labeled content for all analyzed samples, and the enantiomeric impurity was found to be below the limit of detection (LOD), showing the potential of the poly(MQD-co-HEMA-co-EDMA) monolithic column as a reliable tool for the quality control of l-carnosine in dietary supplements by micro-LC. PMID:26410182

  10. Coarse-Grained Modeling of Genetic Circuits as a Function of the Inherent Time Scales

    CERN Document Server

    Labavic, Darka; Hildegard, Wolfhard Janke; Meyer-Ortmanns,

    2012-01-01

    From a coarse-grained perspective the motif of a self-activating species, activating a second species which acts as its own repressor, is widely found in biological systems, in particular in genetic systems with inherent oscillatory behavior. Here we consider a specific realization of this motif as a genetic circuit, in which genes are described as directly producing proteins, leaving out the intermediate step of mRNA production. We focus on the effect that inherent time scales on the underlying fine-grained scale can have on the bifurcation patterns on a coarser scale in time. Time scales are set by the binding and unbinding rates of the transcription factors to the promoter regions of the genes. Depending on the ratio of these rates to the decay times of the proteins, the appropriate averaging procedure for obtaining a coarse-grained description changes and leads to sets of deterministic equations, which differ in their bifurcation structure. In particular the desired intermediate range of regular limit cyc...

  11. Multiple Time Scale Analysis of River Runoff Using Wavelet Transform for Dagujia River Basin, Yantai, China

    Institute of Scientific and Technical Information of China (English)

    LIU Delin; LIU Xianzhao; LI Bicheng; ZHAO Shiwei; LI Xiguo

    2009-01-01

    Based on monOdy river runoff and meteorological data, a method of Morlet wavelet transform was used to analyze the multiple time scale characteristics of river runoffin the Dagnjia River Basin, Yantai City, Shandong Province. The results showed that the total annual river runoff in the Dagujia River Basin decreased significantly from 1966 to 2004, and the rate of decrease was 48×106m3/10yr, which was higher than the mean value of most rivers in China. Multiple time scale characteristics existed, which accounted for different aspects of the changes in annual river runoff, and the major periods of the runoff time series were identified as about 28 years, 14 years and 4 years with decreasing levels of fluctuation. The river runoff evolution process was controlled by changes in precipitation to a certain extent, but it was also greatly influenced by human activities. Also, for different time periods and scales, the impacts of climate changes and human activities on annual river runoff evolution occurred at the same time. Changes in the annual river runoffwere mainly associated with climate change before the 1980s and with human activities after 1981.

  12. A time-scale sensitometric method for evaluating screen - film systems

    Energy Technology Data Exchange (ETDEWEB)

    Goes, E.G.; Pela, C.A.; Ghilardi, N.T. [CIDRA - Centro de Instrumentacao, Dosimetria e Radioprotecao, DFM - FFCLRP - USP, Av. Bandeirantes, 3900-14040 901 Ribeirao Preto, SP (Brazil)

    1997-10-01

    An x-ray sensitometer is used to measure the characteristic curve of radiographic films exposed with fluorescent intensifying screens. The series of relative exposures, necessary to cover the full density range of the film, can be obtained by either time-scale or intensity-scale sensitometric methods. We have developed a convenient method of exposing film-screen systems for time-scale sensitometry. In this method, during exposure the x-ray kilovoltage, tube current and x-ray intensity remain constant and a geometric series of exposures of the film is modulated by varying the exposure time. This time variation can be obtained when a lead disc with different sector openings is rotated in front of the film system by a stepping motor. The conditions normally used are 70 kVp x-rays, 3.5 mm Al total filtration at the tube, and 2.4 m focal spot-film distance. This exposure latitude gives a complete characteristic curve of film-screen systems. (author)

  13. A time-scale sensitometric method for evaluating screen - film systems

    Science.gov (United States)

    Góes, E. G.; Pelá, C. A.; Ghilardi, Netto T.

    1997-10-01

    An x-ray sensitometer is used to measure the characteristic curve of radiographic films exposed with fluorescent intensifying screens. The series of relative exposures, necessary to cover the full density range of the film, can be obtained by either time-scale or intensity-scale sensitometric methods. We have developed a convenient method of exposing film - screen systems for time-scale sensitometry. In this method, during exposure the x-ray kilovoltage, tube current and x-ray intensity remain constant and a geometric series of exposures of the film is modulated by varying the exposure time. This time variation can be obtained when a lead disc with different sector openings is rotated in front of the film system by a stepping motor. The conditions normally used are 70 kVp x-rays, 3.5 mm Al total filtration at the tube, and 2.4 m focal spot - film distance. This exposure latitude gives a complete characteristic curve of film - screen systems.

  14. Modulation of SST, SSS over northern Bay of Bengal on ISO time scale

    Science.gov (United States)

    Rao, Suryachandra A.; Saha, Subodh K.; Pokhrel, Samir; Sundar, Damodaran; Dhakate, Ashish R.; Mahapatra, Somnath; Ali, Sabeer; Chaudhari, Hemantkumar S.; Shreeram, P.; Vasimalla, Suneel; Srikanth, A. S.; Suresh, R. R. V.

    2011-09-01

    High resolution observations of atmospheric and oceanic variables are carried out at northern Bay of Bengal from 22nd July to 6th August 2009 on-board ORV Sagar kanya under the Continental Tropical Convergence Zone research/observational programme. Freshening of surface layer by more than 4 psu within 24 hours is observed, which is followed by warming in the surface layer temperature. The heat and salt budget analysis primarily indicates dominant role of advection processes on the evolution of temperature and salinity. The amount of rainfall received at observation site could not explain the observed freshening, thus an extensive analysis using wavelet coherence is done to find out the source of advected fresh water to the observed location. It is found that surface salinity in the northern Bay of Bengal (at 15° N) varies coherently with the rainfall over Ganga-Brahmaputra catchment area on intraseasonal time scale and with lag of about 60 days. Based on above observations, this study hypothesize that the intraseasonal rainfall variation modulates the amount of river discharge, which in turn modulates the salinity over northern Bay of Bengal on intraseasonal time scale. Since surface warming always follows the surface freshening, the time delay between the rainfall over catchment area and freshening at northern Bay of Bengal may explain the post monsoon warming. Importance of atmosphere-ocean coupling in driving the dynamics of the northern bay of Bengal has been clearly brought out in this study.

  15. Time-Scale Domain Characterization of Time-Varying Ultrawideband Infostation Channel

    Directory of Open Access Journals (Sweden)

    U.A.K. Chude-Okonkwo

    2012-06-01

    Full Text Available The time-scale domain geometrical-based method for the characterization of the time varying ultrawideband (UWB channel typical of an infostation channel is presented. Compared to methods that use Doppler shift as a measure of time-variation in the channel this model provides a more reliable measure of frequency dispersion caused by terminal mobility in the UWB infostation channel. Particularly, it offers carrier frequency independent method of computing wideband channel responses and parameters which are important for ultrawideband systems. Results show that the frequency dispersion of the channel depends on the frequency and not on the choice of bandwidth. And time dispersion depends on bandwidth and not on the frequency. It is also shown that for time-varying UWB, frame length defined over the coherence time obtained with reference to the carrier frequency results in an error margin which can be reduced by using the coherence time defined with respect to the maximum frequency in a given frequency band. And the estimation of the frequency offset using the time-scale domain (wideband model presented here (especially in the case of multiband UWB frequency synchronization is more accurate than using frequency offset estimate obtained from narrowband models.

  16. Formation of the Kuiper Belt by Long Time-Scale Migration of Jovian Planets

    Institute of Scientific and Technical Information of China (English)

    Jian Li; Li-Yong Zhou; Yi-Sui Sun

    2006-01-01

    The orbital migration of Jovian planets is believed to have played an important role in shaping the Kuiper Belt. We investigate the effects of the long time-scale (2 × 107 yr) migration of Jovian planets on the orbital evolution of massless test particles that are initially located beyond 28 AU. Because of the slowness of the migration, Neptune's mean motion resonances capture test particles very efficiently. Taking into account the stochastic behavior during the planetary migration and for proper parameter values, the resulting concentration of objects in the 3:2 resonance is prominent, while very few objects enter the 2:1 resonance,thus matching the observed Kuiper Belt objects very well. We also find that such a long time-scale migration is favorable for exciting the inclinations of the test particles, because it makes the secular resonance possible to operate during the migration. Our analyses show that the v8 secular resonance excites the eccentricities of some test particles, so decreasing their perihelion distances, leading to close encounters with Neptune, which can then pump the inclinations up to 20°.

  17. Finite-difference time-domain simulation of fusion plasmas at radiofrequency time scales

    International Nuclear Information System (INIS)

    Simulation of dense plasmas in the radiofrequency range are typically performed in the frequency domain, i.e., by solving Laplace-transformed Maxwell's equations. This technique is well-suited for the study of linear heating and quasilinear evolution, but does not generalize well to the study of nonlinear phenomena. Conversely, time-domain simulation in this range is difficult because the time scale is long compared to the electron plasma wave period, and in addition, the various cutoff and resonance behaviors within the plasma insure that any explicit finite-difference scheme would be numerically unstable. To resolve this dilemma, explicit finite-difference Maxwell terms are maintained, but a carefully time-centered locally implicit method is introduced to treat the plasma current, such that all linear plasma dispersion behavior is faithfully reproduced at the available temporal and spatial resolution, despite the fact that the simulation time step may exceed the electron gyro and electron plasma time scales by orders of magnitude. Demonstrations are presented of the method for several classical benchmarks, including mode conversion to ion cyclotron wave, cyclotron resonance, propagation into a plasma-wave cutoff, and tunneling through low-density edge plasma

  18. Asymptotic properties of quantum dynamics in bounded domains at various time scales

    International Nuclear Information System (INIS)

    We study a peculiar semiclassical limit of the dynamics of quantum states on a circle and in a box (infinitely deep potential well with rigid walls) as the Planck constant tends to zero and time tends to infinity. Our results describe the dynamics of coherent states on the circle and in the box at all time scales in semiclassical approximation. They give detailed information about all stages of quantum evolution in the semiclassical limit. In particular, we rigorously justify the fact that the spatial distribution of a wave packet is most often close to a uniform distribution. This fact was previously known only from numerical experiments. We apply the results obtained to a problem of classical mechanics: deciding whether the recently suggested functional formulation of classical mechanics is preferable to the traditional one. To do this, we study the semiclassical limit of Husimi functions of quantum states. Both formulations of classical mechanics are shown to adequately describe the system when time is not arbitrarily large. But the functional formulation remains valid at larger time scales than the traditional one and, therefore, is preferable from this point of view. We show that, although quantum dynamics in finite volume is commonly believed to be almost periodic, the probability distribution of the position of a quantum particle in a box has a limit distribution at infinite time if we take into account the inaccuracy in measuring the size of the box.

  19. Variability of Hydroclimate Extremes on Seasonal to Multidecadal Time Scales in the Western US

    Science.gov (United States)

    Bracken, C.; Rajagopalan, B.; Gangopadhyay, S.

    2013-12-01

    Understanding the variability of flood risk on seasonal to multidecadal time scales is critical for a number of activities - such as infrastructure and water resources management, flood mitigation etc. This need is underscored in the Western US with the confluence of socio-economic growth leading to large potential flood damage and water quality impacts and stressed water resources. In this study we perform a systematic analysis of precipitation and streamflow extremes and their links to large scale climate variables. We perform a joint analysis using time and spectral domain methods between seasonal maximum precipitation and large scale climate variables such as sea surface temperatures and sea level pressures. The leading modes from these analyses will identify dominant patterns of variability in space and time. We also obtain insights into the moisture source and delivery mechanisms to various parts of Western US that produce extreme flooding events. We perform similar analysis on seasonal maximum flow to identify consistent mechanism. Existing methods for estimation of risk of extremes is based on extreme value analysis (EVA) assuming stationarity. Clearly the risk of extremes varies in time and space as a function of the strength of the strength of their drivers. Nonstationary EVA methods are emerging and we will apply these to model seasonal precipitation extremes in space incorporating the physical mechanisms identified from the analysis above. This modeling approach can generate nonstationary precipitation and flood frequency estimates at seasonal to multidecadal time scales for infrastructure operations, design and maintenance decisions.

  20. Interdecadal Variations of Phase Delays Between Two Ni(n)o Indices at Different Time Scales

    Institute of Scientific and Technical Information of China (English)

    BIAN Jianchun; YANG Peicai

    2005-01-01

    Phase delays between two Nino indices-sea surface temperatures in Nino regions 1+2 and 3.4(1950-2001)-at different time scales are detected by wavelet analysis. Analysis results show that thereare two types of period bifurcations in the Nino indices and that period bifurcation points exist only in the region where the wavelet power is small. Interdecadal variation features of phase delays between the two indices vary with different time scales. In the periods of 40-72 months, the phase delay changes its sign in 1977: Nino 1+2 indices are 2-4 months earlier than Nino 3.4 indices before 1977, but 3-6 months later afterwards. In the periods of 20-40 months, however, the phase delay changes its sign in another way:Nino 1+2 indices are 1-4 months earlier before 1980 and during 1986-90, but 1-4 months later during 1980-83 and 1993-2001.

  1. Characterization of Microbial Fuel Cells at Microbially and Electrochemically Meaningful Time scales

    KAUST Repository

    Ren, Zhiyong

    2011-03-15

    The variable biocatalyst density in a microbial fuel cell (MFC) anode biofilm is a unique feature of MFCs relative to other electrochemical systems, yet performance characterizations of MFCs typically involve analyses at electrochemically relevant time scales that are insufficient to account for these variable biocatalyst effects. This study investigated the electrochemical performance and the development of anode biofilm architecture under different external loadings, with duplicate acetate-fed singlechamber MFCs stabilized at each resistance for microbially relevant time scales. Power density curves from these steady-state reactors generally showed comparable profiles despite the fact that anode biofilm architectures and communities varied considerably, showing that steady-state biofilm differences had little influence on electrochemical performance until the steady-state external loading was much larger than the reactor internal resistance. Filamentous bacteria were dominant on the anodes under high external resistances (1000 and 5000 Ω), while more diverse rod-shaped cells formed dense biofilms under lower resistances (10, 50, and 265 Ω). Anode charge transfer resistance decreased with decreasing fixed external resistances, but was consistently 2 orders of magnitude higher than the resistance at the cathode. Cell counting showed an inverse exponential correlation between cell numbers and external resistances. This direct link ofMFCanode biofilm evolution with external resistance and electricity production offers several operational strategies for system optimization. © 2011 American Chemical Society.

  2. An 8 hour characteristic time-scale in submillimetre light curves of Sagittarius A*

    CERN Document Server

    Dexter, Jason; Bower, Geoffrey C; Marrone, Daniel P; Stone, Jordan; Plambeck, Richard; Doeleman, Sheperd S

    2013-01-01

    We compile and analyse long term (~10 year) submillimetre (1.3, 0.87, 0.43 mm, submm) wavelength light curves of the Galactic centre black hole, Sagittarius A*. The 0.87 and 0.43 mm data are taken from the literature, while the majority of the 1.3 mm light curve is from previously unpublished SMA and CARMA data. We use Monte Carlo simulations to show that on minute to few hour time-scales the variability is consistent with a red noise process with a 230 GHz power spectrum slope of 2.3+0.8-0.6 at 95% confidence. The light curve is de-correlated (white noise) on very long (month to year) times. In order to identify the transition time between red and white noise, we model the light curves as a stochastic damped random walk process. The models allow a quantitative estimate of this physical characteristic time-scale of 8-4+3 hours at 230 GHz at 95% confidence, with consistent results at 345 and 690 GHz. This corresponds to ~10 orbital times or ~1 inflow (viscous) time at R = 3 Rs, a typical radius producing the 2...

  3. Long time-scale variability of X-ray binaries with late type giant companions

    CERN Document Server

    Filippova, E; Parkin, E R

    2013-01-01

    In this paper we propose and examine a physical mechanism which can lead to the generation of noise in the mass accretion rate of low mass X-ray binaries on time-scales comparable to the orbital period of the system. We consider modulations of mass captured by the compact object from the companion star's stellar wind in binaries with late type giants, systems which usually have long orbital periods. We show that a hydrodynamical interaction of the wind matter within a binary system even without eccentricity results in variability of the mass accretion rate with characteristic time-scales close to the orbital period. The cause of the variability is an undeveloped turbulent motion (perturbed motion without significant vorticity) of wind matter near the compact object. Our conclusions are supported by 3D simulations with two different hydrodynamic codes based on Lagrangian and Eulerian approaches -- the SPH code GADGET and the Eulerian code PLUTO. In this work we assume that the wind mass loss rate of the second...

  4. Cromatografia unificada Unified chromatography

    Directory of Open Access Journals (Sweden)

    Carin von Mühlen

    2004-10-01

    Full Text Available The scope of this study encompasses an overview of the principles of unified chromatography as well as the principles of chromatographic techniques as applied to unified systems, which include gas chromatography, liquid chromatography, supercritical fluid chromatography, high temperature and high pressure liquid chromatography, micro-liquid chromatography, enhanced fluidity chromatography, and solvating gas chromatography. Theoretical considerations and individual instrumental parameters such as mobile phase, sample introduction system, columns, and detection system are also discussed. Future applications of this separation approach are discussed.

  5. Peptidomic Identification of Serum Peptides Diagnosing Preeclampsia.

    Directory of Open Access Journals (Sweden)

    Qiaojun Wen

    Full Text Available We sought to identify serological markers capable of diagnosing preeclampsia (PE. We performed serum peptide analysis (liquid chromatography mass spectrometry of 62 unique samples from 31 PE patients and 31 healthy pregnant controls, with two-thirds used as a training set and the other third as a testing set. Differential serum peptide profiling identified 52 significant serum peptides, and a 19-peptide panel collectively discriminating PE in training sets (n = 21 PE, n = 21 control; specificity = 85.7% and sensitivity = 100% and testing sets (n = 10 PE, n = 10 control; specificity = 80% and sensitivity = 100%. The panel peptides were derived from 6 different protein precursors: 13 from fibrinogen alpha (FGA, 1 from alpha-1-antitrypsin (A1AT, 1 from apolipoprotein L1 (APO-L1, 1 from inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4, 2 from kininogen-1 (KNG1, and 1 from thymosin beta-4 (TMSB4. We concluded that serum peptides can accurately discriminate active PE. Measurement of a 19-peptide panel could be performed quickly and in a quantitative mass spectrometric platform available in clinical laboratories. This serum peptide panel quantification could provide clinical utility in predicting PE or differential diagnosis of PE from confounding chronic hypertension.

  6. Watching the grin fade: tracing the effects of polyploidy on different evolutionary time scales.

    Science.gov (United States)

    Mayfield-Jones, Dustin; Washburn, Jacob D; Arias, Tatiana; Edger, Patrick P; Pires, J Chris; Conant, Gavin C

    2013-04-01

    Polyploidy, or whole-genome duplication (WGD), is a recurrent mutation both in cell lineages and over evolutionary time. By globally changing the relationship between gene copy number and other cellular entities, it can induce dramatic changes at the cellular and phenotypic level. Perhaps surprisingly, then, the insights that these events can bring to understanding other cellular features are not as well appreciated as they could be. In this review, we draw on examples of polyploidy from animals, plants and yeast to explore how investigations of polyploid cells have improved our understanding of the cell cycle, biological network complexity, metabolic phenotypes and tumor biology. We argue that the study of polyploidy across organisms, cell types, and time scales serves not only as a window into basic cell biology, but also as a basis for a predictive biology with applications ranging from crop improvement to treating cancer.

  7. Exponential Stability of Neutral Stochastic Functional Differential Equations with Two-Time-Scale Markovian Switching

    Directory of Open Access Journals (Sweden)

    Junhao Hu

    2014-01-01

    Full Text Available We develop exponential stability of neutral stochastic functional differential equations with two-time-scale Markovian switching modeled by a continuous-time Markov chain which has a large state space. To overcome the computational effort and the complexity, we split the large-scale system into several classes and lump the states in each class into one class by the different states of changes of the subsystems; then, we give a limit system to effectively “replace” the large-scale system. Under suitable conditions, using the stability of the limit system as a bridge, the desired asymptotic properties of the large-scale system with Brownian motion and Poisson jump are obtained by utilizing perturbed Lyapunov function methods and Razumikhin-type criteria. Two examples are provided to demonstrate our results.

  8. Time-resolved and time-scale adaptive measures of spike train synchrony

    CERN Document Server

    Kreuz, Thomas; Greschner, Martin; Andrzejak, Ralph G

    2010-01-01

    A wide variety of approaches to estimate the degree of synchrony between two or more spike trains have been proposed. One of the most recent methods is the ISI-distance which extracts information from the interspike intervals (ISIs) by evaluating the ratio of the instantaneous firing rates. In contrast to most previously proposed measures it is parameter free and time-scale independent. However, it is not well suited to track changes in synchrony that are based on spike coincidences. Here we propose the SPIKE-distance, a complementary measure which is sensitive to spike coincidences but still shares the fundamental advantages of the ISI-distance. In particular, it is easy to visualize in a time-resolved manner and can be extended to a method that is also applicable to larger sets of spike trains. We show the merit of the SPIKE-distance using both simulated and real data.

  9. Attractors of relaxation discrete-time systems with chaotic dynamics on a fast time scale.

    Science.gov (United States)

    Maslennikov, Oleg V; Nekorkin, Vladimir I

    2016-07-01

    In this work, a new type of relaxation systems is considered. Their prominent feature is that they comprise two distinct epochs, one is slow regular motion and another is fast chaotic motion. Unlike traditionally studied slow-fast systems that have smooth manifolds of slow motions in the phase space and fast trajectories between them, in this new type one observes, apart the same geometric objects, areas of transient chaos. Alternating periods of slow regular motions and fast chaotic ones as well as transitions between them result in a specific chaotic attractor with chaos on a fast time scale. We formulate basic properties of such attractors in the framework of discrete-time systems and consider several examples. Finally, we provide an important application of such systems, the neuronal electrical activity in the form of chaotic spike-burst oscillations.

  10. Is there a break in scaling on centennial time scale in Holocene temperature records?

    Science.gov (United States)

    Nilsen, Tine; Rypdal, Kristoffer; Fredriksen, Hege-Beate

    2015-04-01

    A variety of paleoclimatic records have been used to study scaling properties of past climate, including ice core paleotemperature records and multi-proxy reconstructions. Records extending further back in time than the Holocene are divided into glacial/interglacial segments before analysis. The methods used to infer the scaling include the power spectral density (Lomb-Scargle periodogram and standard periodogram), detrended fluctuation analysis, wavelet variance analysis and the Haar fluctuation function. All the methods have individual strengths, weaknesses, uncertainties and biases, and for this reason it is useful to compare results from different methods when possible. Proxy-based reconstructions have limited spatial and temporal coverage, and must be used and interpreted with great care due to uncertainties. By elaborating on physical mechanisms for the actual climate fluctuations seen in the paleoclimatic temperature records as well as uncertainties in both data and methods, we demonstrate the possible pitfalls that may lead to the conclusion that the variability in temperature time series can be separated into different scaling regimes. Categorizing the Earth's surface temperature variability into a «macroweather» and "climate" regime has little or no practical meaning since the different components in the climate system are connected and interact on all time scales. Our most important result is that a break between two different scaling regimes at time scales around one century cannot be identified in Holocene climate. We do, however, observe departures from scaling, which can be attributed to variability such as a single internal quasi-periodic oscillation, an externally forced trend, or a combination of factors. If two scaling regimes are claimed to be present in one single time series, both regimes must be persistent. We show that the limited temporal resolution/length of the records significantly lowers the confidence for such persistence. A total of

  11. Singular perturbations and time scales in the design of digital flight control systems

    Science.gov (United States)

    Naidu, Desineni S.; Price, Douglas B.

    1988-01-01

    The results are presented of application of the methodology of Singular Perturbations and Time Scales (SPATS) to the control of digital flight systems. A block diagonalization method is described to decouple a full order, two time (slow and fast) scale, discrete control system into reduced order slow and fast subsystems. Basic properties and numerical aspects of the method are discussed. A composite, closed-loop, suboptimal control system is constructed as the sum of the slow and fast optimal feedback controls. The application of this technique to an aircraft model shows close agreement between the exact solutions and the decoupled (or composite) solutions. The main advantage of the method is the considerable reduction in the overall computational requirements for the evaluation of optimal guidance and control laws. The significance of the results is that it can be used for real time, onboard simulation. A brief survey is also presented of digital flight systems.

  12. Modeling and Control of a Large Nuclear Reactor A Three-Time-Scale Approach

    CERN Document Server

    Shimjith, S R; Bandyopadhyay, B

    2013-01-01

    Control analysis and design of large nuclear reactors requires a suitable mathematical model representing the steady state and dynamic behavior of the reactor with reasonable accuracy. This task is, however, quite challenging because of several complex dynamic phenomena existing in a reactor. Quite often, the models developed would be of prohibitively large order, non-linear and of complex structure not readily amenable for control studies. Moreover, the existence of simultaneously occurring dynamic variations at different speeds makes the mathematical model susceptible to numerical ill-conditioning, inhibiting direct application of standard control techniques. This monograph introduces a technique for mathematical modeling of large nuclear reactors in the framework of multi-point kinetics, to obtain a comparatively smaller order model in standard state space form thus overcoming these difficulties. It further brings in innovative methods for controller design for systems exhibiting multi-time-scale property,...

  13. Mixed-Mode Oscillations in a piecewise linear system with multiple time scale coupling

    Science.gov (United States)

    Fernández-García, S.; Krupa, M.; Clément, F.

    2016-10-01

    In this work, we analyze a four dimensional slow-fast piecewise linear system with three time scales presenting Mixed-Mode Oscillations. The system possesses an attractive limit cycle along which oscillations of three different amplitudes and frequencies can appear, namely, small oscillations, pulses (medium amplitude) and one surge (largest amplitude). In addition to proving the existence and attractiveness of the limit cycle, we focus our attention on the canard phenomena underlying the changes in the number of small oscillations and pulses. We analyze locally the existence of secondary canards leading to the addition or subtraction of one small oscillation and describe how this change is globally compensated for or not with the addition or subtraction of one pulse.

  14. A Mechanism for Stabilization of Dynamics in Nonlinear Systems with Different Time Scales

    CERN Document Server

    Lopez, Raquel M; Camacho, Erika T

    2009-01-01

    There are many natural, physical, and biological systems that exhibit multiple time scales. For example, the dynamics of a population of ticks can be described in continuous time during their individual life cycle yet discrete time is used to describe the generation of offspring. These characteristics cause the population levels to be reset periodically. A similar phenomenon can be observed in a sociological college drinking model in which the population is reset by the incoming class each year, as described in the 2006 work of Camacho et al. With the latter as our motivation we analytically and numerically investigate the mechanism by which solutions in certain systems with this resetting conditions stabilize. We further utilize the sociological college drinking model as an analogue to analyze certain one-dimensional and two-dimensional nonlinear systems, as we attempt to generalize our results to higher dimensions.

  15. Attractors of relaxation discrete-time systems with chaotic dynamics on a fast time scale

    Science.gov (United States)

    Maslennikov, Oleg V.; Nekorkin, Vladimir I.

    2016-07-01

    In this work, a new type of relaxation systems is considered. Their prominent feature is that they comprise two distinct epochs, one is slow regular motion and another is fast chaotic motion. Unlike traditionally studied slow-fast systems that have smooth manifolds of slow motions in the phase space and fast trajectories between them, in this new type one observes, apart the same geometric objects, areas of transient chaos. Alternating periods of slow regular motions and fast chaotic ones as well as transitions between them result in a specific chaotic attractor with chaos on a fast time scale. We formulate basic properties of such attractors in the framework of discrete-time systems and consider several examples. Finally, we provide an important application of such systems, the neuronal electrical activity in the form of chaotic spike-burst oscillations.

  16. Control of modular multilevel converters based on time-scale analysis and orthogonal functions

    DEFF Research Database (Denmark)

    Zarri, L.; Tani, A.; Mengoni, M.;

    2014-01-01

    Modular multilevel converter (MMC) is a promising multilevel topology for high-voltage applications that has been developed in recent years. The control of MMCs has been analyzed in detail in many papers, showing that the converter capacitors can be kept charged and balanced by controlling the...... circulating current of each leg. The set-point signal of the circulating current is probably the most difficult variable to choose. Typically, it is chosen in such a way to control the total energy and the energy unbalance of each leg. Although several theories are available, the control of the circulating...... current is still a complex task and cannot be fully tackled with traditional linear control techniques. In this paper a multiple time-scale analysis is proposed to determine an approximated model of the MMC that can be used to solve the control problem of the capacitor voltages. In addition, it is shown...

  17. Generalization of the DLA process with different immiscible components by time-scale coarse graining

    Energy Technology Data Exchange (ETDEWEB)

    Postnikov, E B [Kursk State University, Theoretical Physics Department, Radishcheva st, 33, 305000, Kursk (Russian Federation); Ryabov, A B [MV Lomonosov Moscow State University, Physics Faculty, 119992 Moscow (Russian Federation); Loskutov, A [MV Lomonosov Moscow State University, Physics Faculty, 119992 Moscow (Russian Federation)

    2007-10-05

    In the framework of the mean-field approximation we propose a new approach to the description of the growth of fractal structures which are formed as a result of the process of diffusion limited aggregation. Our approach is based on the coarse graining of the time scale which takes into account the property of discreteness of such structures. The obtained system of partial differential equations allows us to evaluate numerically the fractal dimension and the cluster density depending on the distance from the cluster center. The results are in a quite good agreement with values found by the direct numerical simulations. The proposed approach is generalized for the case of the cluster description with different immiscible particles.

  18. Generalization of the DLA process with different immiscible components by time-scale coarse graining

    Science.gov (United States)

    Postnikov, E. B.; Ryabov, A. B.; Loskutov, A.

    2007-10-01

    In the framework of the mean-field approximation we propose a new approach to the description of the growth of fractal structures which are formed as a result of the process of diffusion limited aggregation. Our approach is based on the coarse graining of the time scale which takes into account the property of discreteness of such structures. The obtained system of partial differential equations allows us to evaluate numerically the fractal dimension and the cluster density depending on the distance from the cluster center. The results are in a quite good agreement with values found by the direct numerical simulations. The proposed approach is generalized for the case of the cluster description with different immiscible particles.

  19. Origin of space-separated charges in photoexcited organic heterojunctions on ultrafast time scales

    CERN Document Server

    Janković, Veljko

    2016-01-01

    We present detailed investigation of ultrafast (sub-ps) exciton dynamics in the lattice model of a donor/acceptor heterojunction. Exciton generation by means of a photoexcitation, exciton dissociation, and further charge separation are treated on equal footing. The experimentally observed presence of space-separated charges at $\\lesssim 100$ fs after the photoexcitation is usually attributed to ultrafast transitions from excitons in the donor to charge transfer and charge separated states. Here, we show, however, that space-separated charges appearing on $\\lesssim 100$-fs time scales are predominantly directly optically generated. Our theoretical insights into the ultrafast pump-probe spectroscopy challenge usual interpretations of pump-probe spectra in terms of ultrafast population transfer from donor excitons to space-separated charges.

  20. On simulating flow with multiple time scales using a method of averages

    Energy Technology Data Exchange (ETDEWEB)

    Margolin, L.G. [Los Alamos National Lab., NM (United States)

    1997-12-31

    The author presents a new computational method based on averaging to efficiently simulate certain systems with multiple time scales. He first develops the method in a simple one-dimensional setting and employs linear stability analysis to demonstrate numerical stability. He then extends the method to multidimensional fluid flow. His method of averages does not depend on explicit splitting of the equations nor on modal decomposition. Rather he combines low order and high order algorithms in a generalized predictor-corrector framework. He illustrates the methodology in the context of a shallow fluid approximation to an ocean basin circulation. He finds that his new method reproduces the accuracy of a fully explicit second-order accurate scheme, while costing less than a first-order accurate scheme.

  1. Continent-scale global change attribution in European birds - combining annual and decadal time scales

    DEFF Research Database (Denmark)

    Jørgensen, Peter Søgaard; Böhning-Gaese, Katrin; Thorup, Kasper;

    2016-01-01

    investigate the recent impact of multiple environmental changes on European farmland birds, here focusing on climate change and land use change. We analyze more than 800 time series from 18 countries spanning the past two decades. Analysis of long-term population growth rates documents simultaneous responses......Species attributes are commonly used to infer impacts of environmental change on multiyear species trends, e.g. decadal changes in population size. However, by themselves attributes are of limited value in global change attribution since they do not measure the changing environment. A broader...... foundation for attributing species responses to global change may be achieved by complementing an attributes-based approach by one estimating the relationship between repeated measures of organismal and environmental changes over short time scales. To assess the benefit of this multiscale perspective, we...

  2. The mechanism of bursting phenomena in Belousov-Zhabotinsky(BZ) chemical reaction with multiple time scales

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    The dynamics of a typical Belousov-Zhabotinsky(BZ)reaction with multiple time scales is investigated in this paper.Different forms of periodic bursting phenomena,and specially,three types of chaotic bursters with different structures can be obtained,which are in common with the behaviors observed in experiments.The bifurcations connecting the quiescent state and the repetitive spikes are presented to account for the occurrence of the NKoscillations as well as the different forms of chaotic bursters.The mechanism of the period-adding bifurcation sequences is explored to reveal why the length of the periods in the sequences does not change continuously with the continuous variation of the parameters.

  3. Fluctuations and time scales for bed-load sediment motion over a smooth bed

    Institute of Scientific and Technical Information of China (English)

    Francesco Ballio n; Alessio Radice

    2015-01-01

    Results are presented for experiments of bed-load sediment transport over a plane, smooth bed. The smooth-bed configuration, though not adequate for mimicking natural streams, enables the effects of bed roughness to be filtered out, thus, highlighting the role of flow turbulence for particle dynamics. Sediments were individually tracked along their paths, measuring position and velocity of the individual grains. A number of analyses were then applied to the data: probability density function, auto-correla-tion, and spectra of the grain velocity. Several Lagrangian time scales of particle motion were obtained and compared to available data for the turbulent flow field to determine a phenomenological inter-pretation of the process.

  4. Impulsive Periodic Boundary Value Problems for Dynamic Equations on Time Scale

    Directory of Open Access Journals (Sweden)

    Eric R. Kaufmann

    2009-01-01

    Full Text Available Let 𝕋 be a periodic time scale with period p such that 0,ti,T=mp∈𝕋, i=1,2,…,n, m∈ℕ, and 0

  5. Evidence of Invariance of Time Scale at Critical Point in Ising Meanfield Equilibrium Equation of State

    Institute of Scientific and Technical Information of China (English)

    Muktish Acharyya; Ajanta Bhowal Acharyya

    2011-01-01

    We solve the equilibrium meanfield equation of state of Ising ferromagnet (obtained from Bragg-Williams theory) by Newton-Raphson method.The number of iterations required to get a convergent solution (within a specified accuracy) of equilibrium magnetisation, at any particular temperature, is observed to diverge in a power law fashion as the temperature approaches the critical value.This is identified as the critical slowing down.The exponent is also estimated.This value of the exponent is compared with that obtained from analytic solution.Besides this, the numerical results are also compared with some experimental results exhibiting satisfactory degree of agreement.It is observed from this study that the information of the invariance of time scale at the critical point is present in the meanfield equilibrium equation of state of Ising ferromagnet.

  6. Existence of positive solutions for semipositone dynamic system on time scales

    Directory of Open Access Journals (Sweden)

    You-Wei Zhang

    2008-08-01

    Full Text Available In this paper, we study the following semipositone dynamic system on time scales $$displaylines{ -x^{DeltaDelta}(t=f(t,y+p(t, quad tin(0,T_{mathbb{T}},cr -y^{DeltaDelta}(t=g(t,x, quad tin(0,T_{mathbb{T}},cr x(0=x(sigma^{2}(T=0, cr alpha{y(0}-eta{y^{Delta}{(0}}= gamma{y(sigma(T}+delta{y^{Delta}(sigma(T}=0. }$$ Using fixed point index theory, we show the existence of at least one positive solution. The interesting point is the that nonlinear term is allowed to change sign and may tend to negative infinity.

  7. Positive Solutions for Second-Order m-Point Boundary Value Problems on Time Scales

    Institute of Scientific and Technical Information of China (English)

    Wan Tong LI; Hong Rui SUN

    2006-01-01

    Let T be a time scale such that 0, T ∈ T. By means of the Schauder fixed point theorem and analysis method, we establish some existence criteria for positive solutions of the m-point boundary value problem on time scaleswhere a ∈ Cld((0, T),[0,∞)), f ∈ Cld([0, ∞) × [0, ∞),[0, ∞)), β,γ∈ [0, ∞), ξi ∈ (0, ρ(T)), b, ai ∈(0, ∞) (for i = 1,..., m - 2) are given constants satisfying some suitable hypotheses. We show that this problem has at least one positive solution for sufficiently small b > 0 and no solution for sufficiently large b. Our results are new even for the corresponding differential equation (T= R) and difference equation (T = Z).

  8. Intrinsic dynamics of heart regulatory systems on short time-scales: from experiment to modelling

    CERN Document Server

    Khovanov, I A; McClintock, P V E; Stefanovska, A

    2009-01-01

    We discuss open problems related to the stochastic modeling of cardiac function. The work is based on an experimental investigation of the dynamics of heart rate variability (HRV) in the absence of respiratory perturbations. We consider first the cardiac control system on short time scales via an analysis of HRV within the framework of a random walk approach. Our experiments show that HRV on timescales of less than a minute takes the form of free diffusion, close to Brownian motion, which can be described as a non-stationary process with stationary increments. Secondly, we consider the inverse problem of modeling the state of the control system so as to reproduce the experimentally observed HRV statistics of. We discuss some simple toy models and identify open problems for the modelling of heart dynamics.

  9. A characteristic time scale of tick quotes on foreign currency markets

    CERN Document Server

    Sato, A H

    2005-01-01

    This study investigates that a characteristic time scale on an exchange rate market (USD/JPY) is examined for the period of 1998 to 2000. Calculating power spectrum densities for the number of tick quotes per minute and averaging them over the year yield that the mean power spectrum density has a peak at high frequencies. Consequently it means that there exist the characteristic scales which dealers act in the market. A simple agent model to explain this phenomenon is proposed. This phenomena may be a result of stochastic resonance with exogenous periodic information and physiological fluctuations of the agents. This may be attributed to the traders' behavior on the market. The potential application is both quantitative characterization and classification of foreign currency markets.

  10. Effects of demographic stochasticity on biological community assembly on evolutionary time scales

    KAUST Repository

    Murase, Yohsuke

    2010-04-13

    We study the effects of demographic stochasticity on the long-term dynamics of biological coevolution models of community assembly. The noise is induced in order to check the validity of deterministic population dynamics. While mutualistic communities show little dependence on the stochastic population fluctuations, predator-prey models show strong dependence on the stochasticity, indicating the relevance of the finiteness of the populations. For a predator-prey model, the noise causes drastic decreases in diversity and total population size. The communities that emerge under influence of the noise consist of species strongly coupled with each other and have stronger linear stability around the fixed-point populations than the corresponding noiseless model. The dynamics on evolutionary time scales for the predator-prey model are also altered by the noise. Approximate 1/f fluctuations are observed with noise, while 1/ f2 fluctuations are found for the model without demographic noise. © 2010 The American Physical Society.

  11. Psychological Momentum During and Across Sports Matches: Evidence for Interconnected Time Scales.

    Science.gov (United States)

    Den Hartigh, Ruud J; Van Geert, Paul L; Van Yperen, Nico W; Cox, Ralf F; Gernigon, Christophe

    2016-02-01

    This study on psychological momentum (PM) in sports provides the first experimental test of an interconnection between short-term PM (during a match) and long-term PM (across a series of matches). Twenty-two competitive athletes were striving to win a prize during a rowing-ergometer tournament, consisting of manipulated races. As hypothesized, athletes who had developed long-term positive PM after two successful races were less sensitive to a negative momentum scenario in the third race, compared with athletes who had developed long-term negative PM after two unsuccessful races. More specifically, the exerted efforts, perceptions of momentum, and self-efficacy were higher for participants who had developed long-term positive PM, and their perceptions of momentum and self-efficacy decreased less rapidly. These results illustrate a typical complex dynamical systems property, namely interconnected time scales, and provide deeper insights into the dynamical nature of PM. PMID:27018560

  12. Recurrence CFD - a novel approach to simulate multiphase flows with strongly separated time scales

    CERN Document Server

    Lichtenegger, Thomas

    2016-01-01

    Classical Computational Fluid Dynamics (CFD) of long-time processes with strongly separated time scales is computationally extremely demanding if not impossible. Consequently, the state-of-the-art description of such systems is not capable of real-time simulations or online process monitoring. In order to bridge this gap, we propose a new method suitable to decouple slow from fast degrees of freedom in many cases. Based on the recurrence statistics of unsteady flow fields, we deduce a recurrence process which enables the generic representation of pseudo-periodic motion at high spatial and temporal resolution. Based on these fields, passive scalars can be traced by recurrence CFD. While a first, Eulerian Model A solves a passive transport equation in a classical implicit finite-volume environment, a second, Lagrangian Model B propagates fluid particles obeying a stochastic differential equation explicitly. Finally, this new concept is tested by two multiphase processes - a lab scale oscillating bubble column a...

  13. Sediment fining processes in a mountain stream at multiple time scales

    Science.gov (United States)

    Le Bouteiller, Caroline; Mathys, Nicolle; Klotz, Sebastien

    2015-04-01

    Downstream fining of sediment is observed in most gravel bed rivers, and is attributed to two mechanisms. The first one is an apparent fining that results from a collective effect called selective sorting: smaller grains travel further downstream while larger grains deposit preferentially upstream. The second one is generally referred to as abrasion and encompasses all the fining processes that affect each grain during its travel along the stream. The latter type of processes is dominant in the mountainous streams of the Draix observatory and is the focus of this study. Draix catchments are characterized by hard climatic conditions with winter frost and storm-induced floods, and a very erodible lithology (marl). During the floods, at the time scale of a few minutes, sediment size is reduced by surface abrasion and fragmentation due to the collisions between grains. In between the floods, at the time scale of a few weeks to months, sediments that remain exposed on bars at low flow are affected by weathering due to frost/thaw and wetting/drying alternations, which also reduces their size. Using field measurements, we measured the global sediment fining rate that results from both short-term (flood) and long-term (low flow) processes. The very high value obtained (51%/km) reflects the combination of the soft lithology with hard climatic conditions. We then combined various field and laboratory experiments to quantify the efficiency of each fining process (surface abrasion and fragmentation during a flood, frost/thaw weathering and wetting/drying weathering). Results indicate that short-term and long-term processes are equally efficient and that both are needed to explain the in-situ global fining rates. We finally propose a simplified model to describe the observed fining patterns, which we use to predict the system response to changes in the hydrological or climatic regime.

  14. Continent-scale global change attribution in European birds - combining annual and decadal time scales.

    Science.gov (United States)

    Jørgensen, Peter Søgaard; Böhning-Gaese, Katrin; Thorup, Kasper; Tøttrup, Anders P; Chylarecki, Przemysław; Jiguet, Frédéric; Lehikoinen, Aleksi; Noble, David G; Reif, Jiri; Schmid, Hans; van Turnhout, Chris; Burfield, Ian J; Foppen, Ruud; Voříšek, Petr; van Strien, Arco; Gregory, Richard D; Rahbek, Carsten

    2016-02-01

    Species attributes are commonly used to infer impacts of environmental change on multiyear species trends, e.g. decadal changes in population size. However, by themselves attributes are of limited value in global change attribution since they do not measure the changing environment. A broader foundation for attributing species responses to global change may be achieved by complementing an attributes-based approach by one estimating the relationship between repeated measures of organismal and environmental changes over short time scales. To assess the benefit of this multiscale perspective, we investigate the recent impact of multiple environmental changes on European farmland birds, here focusing on climate change and land use change. We analyze more than 800 time series from 18 countries spanning the past two decades. Analysis of long-term population growth rates documents simultaneous responses that can be attributed to both climate change and land-use change, including long-term increases in populations of hot-dwelling species and declines in long-distance migrants and farmland specialists. In contrast, analysis of annual growth rates yield novel insights into the potential mechanisms driving long-term climate induced change. In particular, we find that birds are affected by winter, spring, and summer conditions depending on the distinct breeding phenology that corresponds to their migratory strategy. Birds in general benefit from higher temperatures or higher primary productivity early on or in the peak of the breeding season with the largest effect sizes observed in cooler parts of species' climatic ranges. Our results document the potential of combining time scales and integrating both species attributes and environmental variables for global change attribution. We suggest such an approach will be of general use when high-resolution time series are available in large-scale biodiversity surveys. PMID:26486804

  15. Streamflow response of a small forested catchment on different time scales

    Directory of Open Access Journals (Sweden)

    A. Zabaleta

    2012-08-01

    Full Text Available The hydrological response of a catchment to rainfall on different time scales is result of a complex system involving a range of physical processes which may operate simultaneously and have different spatial and temporal influences. This paper presents the analysis of streamflow response of a small humid-temperate catchment (Aixola, 4.8 km2 in the Basque Country on different time scales and discusses the role of the controlling factors. Firstly, daily time series analysis was used to establish a hypothesis on the general functioning of the catchment through the relationship between precipitation and discharge on an annual and multi-annual scale (2003–2008. Second, rainfall-runoff relationships and relationships among several hydrological variables, including catchment antecedent conditions, were explored at the event scale (222 events to check and improve the hypothesis. Finally, the evolution of electrical conductivity (EC during some of the monitored storm events (28 events was examined to identify the time-origin of waters. Quick response of the catchment to almost all the rainfall events as well as a considerable regulation capacity was deduced from the correlation and spectral analyses. These results agree with runoff event scale data analysis; however the event analysis revealed the non-linearity of the system, as antecedent conditions play a significant role in this catchment. Further, analysis at the event scale made possible to clarify factors controlling (precipitation, precipitation intensity and initial discharge the different aspects of the runoff response (runoff coefficient and discharge increase for this catchment. Finally, the evolution of EC of the waters enabled the time origin (event or pre-event waters of the quickflow to be established; specifically, the conductivity showed that pre-event waters usually represent a high percentage of the total discharge during runoff peaks. The importance of soil waters in the

  16. Climate scenarios for Olkiluoto on a time-scale of 120,000 years

    Energy Technology Data Exchange (ETDEWEB)

    Pimenoff, N.; Venaelaeinen, A.; Jaervinen, H. [Finnish Meteorological Institute, Helsinki (Finland)

    2011-12-15

    Posiva Oy is planning to dispose of spent nuclear fuel in a repository, to be constructed at a depth of 400 m in the crystalline bedrock at Olkiluoto, Finland. Planning the storage requires careful consideration of many aspects, including an assessment of long-term repository safety. For estimating possible climate states at Olkiluoto on a time-scale of 120,000 years, we analyze climate simulations of an Earth System Model of Intermediate Complexity (CLIMBER-2) coupled with an ice sheet model (SICOPOLIS). The simulations into the future clearly show that the onset of the next glaciation is strongly dependent on the Earth's orbital variations and the atmospheric CO{sub 2} concentration. It is evident that due to global warming, the climate of the next centuries will be warmer and wetter than at present. Most likely, due to global warming and low variations in the Earth's orbit around the sun, the present interglacial will last for at least the next 30,000 years. Further, the future simulations showed that the insolation minima on the Northern Hemisphere 50,000-60,000 and 90,000-100,000 years after the present hold a potential for the onset of the next glaciation. Hence, on a time-scale of 120,000 years, one must take into account climate periods lasting several thousand years having the following features: an interglacial climate, a periglacial climate, a climate with an ice sheet margin near Olkiluoto, a glacial climate with an ice sheet covering Olkiluoto, and a climate with Olkiluoto being depressed below sea level after glaciation due to isostatic depression. Due to the uncertainties related to the evolution of the future climate, it is recommended the simulations into the far future to be used only qualitatively. Quantitative information about glacial climate is achieved from the reconstructions and simulations of the past climate. (orig.)

  17. Approximate method for stochastic chemical kinetics with two-time scales by chemical Langevin equations.

    Science.gov (United States)

    Wu, Fuke; Tian, Tianhai; Rawlings, James B; Yin, George

    2016-05-01

    The frequently used reduction technique is based on the chemical master equation for stochastic chemical kinetics with two-time scales, which yields the modified stochastic simulation algorithm (SSA). For the chemical reaction processes involving a large number of molecular species and reactions, the collection of slow reactions may still include a large number of molecular species and reactions. Consequently, the SSA is still computationally expensive. Because the chemical Langevin equations (CLEs) can effectively work for a large number of molecular species and reactions, this paper develops a reduction method based on the CLE by the stochastic averaging principle developed in the work of Khasminskii and Yin [SIAM J. Appl. Math. 56, 1766-1793 (1996); ibid. 56, 1794-1819 (1996)] to average out the fast-reacting variables. This reduction method leads to a limit averaging system, which is an approximation of the slow reactions. Because in the stochastic chemical kinetics, the CLE is seen as the approximation of the SSA, the limit averaging system can be treated as the approximation of the slow reactions. As an application, we examine the reduction of computation complexity for the gene regulatory networks with two-time scales driven by intrinsic noise. For linear and nonlinear protein production functions, the simulations show that the sample average (expectation) of the limit averaging system is close to that of the slow-reaction process based on the SSA. It demonstrates that the limit averaging system is an efficient approximation of the slow-reaction process in the sense of the weak convergence. PMID:27155630

  18. Variations of characteristic time scales in rotating stratified turbulence using a large parametric numerical study.

    Science.gov (United States)

    Rosenberg, D; Marino, R; Herbert, C; Pouquet, A

    2016-01-01

    We study rotating stratified turbulence (RST) making use of numerical data stemming from a large parametric study varying the Reynolds, Froude and Rossby numbers, Re, Fr and Ro in a broad range of values. The computations are performed using periodic boundary conditions on grids of 1024(3) points, with no modeling of the small scales, no forcing and with large-scale random initial conditions for the velocity field only, and there are altogether 65 runs analyzed in this paper. The buoyancy Reynolds number defined as R(B) = ReFr2 varies from negligible values to ≈ 10(5), approaching atmospheric or oceanic regimes. This preliminary analysis deals with the variation of characteristic time scales of RST with dimensionless parameters, focusing on the role played by the partition of energy between the kinetic and potential modes, as a key ingredient for modeling the dynamics of such flows. We find that neither rotation nor the ratio of the Brunt-Väisälä frequency to the inertial frequency seem to play a major role in the absence of forcing in the global dynamics of the small-scale kinetic and potential modes. Specifically, in these computations, mostly in regimes of wave turbulence, characteristic times based on the ratio of energy to dissipation of the velocity and temperature fluctuations, T(V) and T(P), vary substantially with parameters. Their ratio γ=T(V)/T(P) follows roughly a bell-shaped curve in terms of Richardson number Ri. It reaches a plateau - on which time scales become comparable, γ≈0.6 - when the turbulence has significantly strengthened, leading to numerous destabilization events together with a tendency towards an isotropization of the flow. PMID:26830757

  19. Sensitivity of the simulated precipitation to changes in convective relaxation time scale

    Directory of Open Access Journals (Sweden)

    S. K. Mishra

    2010-10-01

    Full Text Available The paper describes the sensitivity of the simulated precipitation to changes in convective relaxation time scale (TAU of Zhang and McFarlane (ZM cumulus parameterization, in NCAR-Community Atmosphere Model version 3 (CAM3. In the default configuration of the model, the prescribed value of TAU, a characteristic time scale with which convective available potential energy (CAPE is removed at an exponential rate by convection, is assumed to be 1 h. However, some recent observational findings suggest that, it is larger by around one order of magnitude. In order to explore the sensitivity of the model simulation to TAU, two model frameworks have been used, namely, aqua-planet and actual-planet configurations. Numerical integrations have been carried out by using different values of TAU, and its effect on simulated precipitation has been analyzed.

    The aqua-planet simulations reveal that when TAU increases, rate of deep convective precipitation (DCP decreases and this leads to an accumulation of convective instability in the atmosphere. Consequently, the moisture content in the lower- and mid- troposphere increases. On the other hand, the shallow convective precipitation (SCP and large-scale precipitation (LSP intensify, predominantly the SCP, and thus capping the accumulation of convective instability in the atmosphere. The total precipitation (TP remains approximately constant, but the proportion of the three components changes significantly, which in turn alters the vertical distribution of total precipitation production. The vertical structure of moist heating changes from a vertically extended profile to a bottom heavy profile, with the increase of TAU. Altitude of the maximum vertical velocity shifts from upper troposphere to lower troposphere. Similar response was seen in the actual-planet simulations. With an increase in TAU from 1 h to 8 h, there was a significant improvement in the simulation of the seasonal mean precipitation. The

  20. Antimicrobial peptides.

    Science.gov (United States)

    Zhang, Ling-Juan; Gallo, Richard L

    2016-01-11

    Antimicrobial peptides and proteins (AMPs) are a diverse class of naturally occurring molecules that are produced as a first line of defense by all multicellular organisms. These proteins can have broad activity to directly kill bacteria, yeasts, fungi, viruses and even cancer cells. Insects and plants primarily deploy AMPs as an antibiotic to protect against potential pathogenic microbes, but microbes also produce AMPs to defend their environmental niche. In higher eukaryotic organisms, AMPs can also be referred to as 'host defense peptides', emphasizing their additional immunomodulatory activities. These activities are diverse, specific to the type of AMP, and include a variety of cytokine and growth factor-like effects that are relevant to normal immune homeostasis. In some instances, the inappropriate expression of AMPs can also induce autoimmune diseases, thus further highlighting the importance of understanding these molecules and their complex activities. This Primer will provide an update of our current understanding of AMPs. PMID:26766224

  1. Antimicrobial Peptides

    Directory of Open Access Journals (Sweden)

    Ali Adem Bahar

    2013-11-01

    Full Text Available The rapid increase in drug-resistant infections has presented a serious challenge to antimicrobial therapies. The failure of the most potent antibiotics to kill “superbugs” emphasizes the urgent need to develop other control agents. Here we review the history and new development of antimicrobial peptides (AMPs, a growing class of natural and synthetic peptides with a wide spectrum of targets including viruses, bacteria, fungi, and parasites. We summarize the major types of AMPs, their modes of action, and the common mechanisms of AMP resistance. In addition, we discuss the principles for designing effective AMPs and the potential of using AMPs to control biofilms (multicellular structures of bacteria embedded in extracellular matrixes and persister cells (dormant phenotypic variants of bacterial cells that are highly tolerant to antibiotics.

  2. Almost periodic dynamics for impulsive delay neural networks of a general type on almost periodic time scales

    Science.gov (United States)

    Wang, Chao; Agarwal, Ravi P.

    2016-07-01

    In this paper, we improve the new concepts of almost periodic time scales addressed in 2014 to introduce the new concepts of piecewise almost periodic functions. Then we establish some first results for the existence and exponential stability of almost periodic solution for a general type of delay neural networks with impulsive effects. The approaches are based on contraction principle and Gronwall-Bellman's inequality. In addition, the numerical example and simulations are provided to illustrate our time scale methods are feasible and effective. The obtained results are more general and comprehensive than the results obtained on periodic time scales before.

  3. Multi-dimensional liquid chromatography in proteomics-A review

    International Nuclear Information System (INIS)

    Proteomics is the large-scale study of proteins, particularly their expression, structures and functions. This still-emerging combination of technologies aims to describe and characterize all expressed proteins in a biological system. Because of upper limits on mass detection of mass spectrometers, proteins are usually digested into peptides and the peptides are then separated, identified and quantified from this complex enzymatic digest. The problem in digesting proteins first and then analyzing the peptide cleavage fragments by mass spectrometry is that huge numbers of peptides are generated that overwhelm direct mass spectral analyses. The objective in the liquid chromatography approach to proteomics is to fractionate peptide mixtures to enable and maximize identification and quantification of the component peptides by mass spectrometry. This review will focus on existing multidimensional liquid chromatographic (MDLC) platforms developed for proteomics and their application in combination with other techniques such as stable isotope labeling. We also provide some perspectives on likely future developments.

  4. Multiple time scales in the microscopic dynamics of simple and complex liquids as studied by radiation scattering

    Directory of Open Access Journals (Sweden)

    F.J.Bermejo

    2008-03-01

    Full Text Available Experimental investigations into the time-scales spanned by the microscopic dynamics of simple (metallic and molecular liquids, as explored by neutron scattering and muon spin rotation experiments are reviewed.

  5. EXISTENCE AND MULTIPLE EXISTENCE OF POSITIVE SOLUTIONS TO SECOND-ORDER m-POINT BOUNDARY VALUE PROBLEM ON TIME SCALES

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    By different fixed point theorems in cones, sufficient conditions for the existence and multiple existence of positive solutions to a class of second-order multi-point boundary value problem for dynamic equation on time scales are obtained.

  6. Necessary and Sufficient Conditions for the Existence of Positive Solution for Singular Boundary Value Problems on Time Scales

    Directory of Open Access Journals (Sweden)

    Zhang Xuemei

    2009-01-01

    Full Text Available By constructing available upper and lower solutions and combining the Schauder's fixed point theorem with maximum principle, this paper establishes sufficient and necessary conditions to guarantee the existence of as well as positive solutions for a class of singular boundary value problems on time scales. The results significantly extend and improve many known results for both the continuous case and more general time scales. We illustrate our results by one example.

  7. Instrumentation: Ion Chromatography.

    Science.gov (United States)

    Fritz, James S.

    1987-01-01

    Discusses the importance of ion chromatography in separating and measuring anions. The principles of ion exchange are presented, along with some applications of ion chromatography in industry. Ion chromatography systems are described, as well as ion pair and ion exclusion chromatography, column packings, detectors, and programming. (TW)

  8. 高效液相色谱法测定牡蛎大豆肽肉碱口服液中维生素B6含量的不确定度评定%Uncertainty evaluation of determination of vitamin B6 content in oyster soybean peptide carnitine oral liquid by high per- formance liquid chromatography

    Institute of Scientific and Technical Information of China (English)

    庞学斌; 张伟; 邱颖姮; 邬晓鸥

    2015-01-01

    Objective Uncertainty evaluation of determination of vitamin B6 content in oyster soybean peptide carnitine oral liquid by high performance liquid chromatography(HPLC assay). Methods HPLC was adopted for the determination of vitamin B6 content. Mathematical model was established for the evaluation of uncertainty to quantize influential factors of uncertainty and evaluate the uncertainty factors further. Results The content of vitamin B6 in sample oral liquid was 0.498 mg/100mL,in which, the relative uncertaintyof vitamin B6 content was 0.024 9,the standard uncertainty was 0.0124 mg/100 mL,the expanded uncertainty was 0.024 8 mg/100 mL(k=2). Conclusion The calculation method is practical and reliable,being access to the uncertainty eval—uation of content of vitamin B6 in oyster soybean peptide carnitine oral liquid by high performance liquid chromatography. Upon the evaluation,it is found that the quantitative repetitive of the HPLC is the major impact.%目的:评定高效液相色谱法(HPLC法)测定牡蛎大豆肽肉碱口服液中维生素B6含量的不确定度。方法采用HPLC法测定试样中维生素B6的含量,根据建测量模型,确定影响不确定度的分量,并加以评定量化,得出合成不确定度和扩展不确定度。结果试样中维生素B6含量测得值为0.498 mg/100 mL,其中B6含量的相对不确定度为0.0249,标准不确定度为0.0124 mg/100 mL,扩展不确定度为0.0248 mg/100 mL(k=2)。结论建立的计算方法实用、可靠,可以用于HPLC法测定牡蛎大豆肽肉碱口服液中维生素B6含量的不确定度评定,经评定发现,高效液相色谱仪定量重复性对不确定度的影响最大。

  9. Observation of quantum particles on a large space-time scale

    Science.gov (United States)

    Landau, L. J.

    1994-10-01

    A quantum particle observed on a sufficiently large space-time scale can be described by means of classical particle trajectories. The joint distribution for large-scale multiple-time position and momentum measurements on a nonrelativistic quantum particle moving freely in R v is given by straight-line trajectories with probabilities determined by the initial momentum-space wavefunction. For large-scale toroidal and rectangular regions the trajectories are geodesics. In a uniform gravitational field the trajectories are parabolas. A quantum counting process on free particles is also considered and shown to converge in the large-space-time limit to a classical counting process for particles with straight-line trajectories. If the quantum particle interacts weakly with its environment, the classical particle trajectories may undergo random jumps. In the random potential model considered here, the quantum particle evolves according to a reversible unitary one-parameter group describing elastic scattering off static randomly distributed impurities (a quantum Lorentz gas). In the large-space-time weak-coupling limit a classical stochastic process is obtained with probability one and describes a classical particle moving with constant speed in straight lines between random jumps in direction. The process depends only on the ensemble value of the covariance of the random field and not on the sample field. The probability density in phase space associated with the classical stochastic process satisfies the linear Boltzmann equation for the classical Lorentz gas, which, in the limit h→0, goes over to the linear Landau equation. Our study of the quantum Lorentz gas is based on a perturbative expansion and, as in other studies of this system, the series can be controlled only for small values of the rescaled time and for Gaussian random fields. The discussion of classical particle trajectories for nonrelativistic particles on a macroscopic spacetime scale applies also to

  10. Cooling and exhumation of continents at billion-year time scales

    Science.gov (United States)

    Blackburn, T.; Bowring, S. A.; Perron, T.; Mahan, K. H.; Dudas, F. O.

    2011-12-01

    more. Deeper xenoliths record cooling at progressively younger times at similar rates and time-scales. From orogony to eruption of xenoliths onto the surface, the lithospheric thermal history constructed using this technique may exceed a billion years. Combining this cooling history with a lithosphere thermal model yields an estimate for the average integrated rate of craton erosion between 0.00-<0.0025 km/Ma across the orogen; a range far lower than the geologically recent to present day rates for continental erosion (<0.005-0.1 km/Ma). This marks the first ever determination of continental exhumation rates on time-scales that approach the age of the continents themselves and has implications for secular cooling of the asthenosphere.

  11. Variability of interconnected wind plants: correlation length and its dependence on variability time scale

    Science.gov (United States)

    St. Martin, Clara M.; Lundquist, Julie K.; Handschy, Mark A.

    2015-04-01

    The variability in wind-generated electricity complicates the integration of this electricity into the electrical grid. This challenge steepens as the percentage of renewably-generated electricity on the grid grows, but variability can be reduced by exploiting geographic diversity: correlations between wind farms decrease as the separation between wind farms increases. But how far is far enough to reduce variability? Grid management requires balancing production on various timescales, and so consideration of correlations reflective of those timescales can guide the appropriate spatial scales of geographic diversity grid integration. To answer ‘how far is far enough,’ we investigate the universal behavior of geographic diversity by exploring wind-speed correlations using three extensive datasets spanning continents, durations and time resolution. First, one year of five-minute wind power generation data from 29 wind farms span 1270 km across Southeastern Australia (Australian Energy Market Operator). Second, 45 years of hourly 10 m wind-speeds from 117 stations span 5000 km across Canada (National Climate Data Archive of Environment Canada). Finally, four years of five-minute wind-speeds from 14 meteorological towers span 350 km of the Northwestern US (Bonneville Power Administration). After removing diurnal cycles and seasonal trends from all datasets, we investigate dependence of correlation length on time scale by digitally high-pass filtering the data on 0.25-2000 h timescales and calculating correlations between sites for each high-pass filter cut-off. Correlations fall to zero with increasing station separation distance, but the characteristic correlation length varies with the high-pass filter applied: the higher the cut-off frequency, the smaller the station separation required to achieve de-correlation. Remarkable similarities between these three datasets reveal behavior that, if universal, could be particularly useful for grid management. For high

  12. Affinity purification of copper chelating peptides from chickpea protein hydrolysates.

    Science.gov (United States)

    Megías, Cristina; Pedroche, Justo; Yust, Maria M; Girón-Calle, Julio; Alaiz, Manuel; Millan, Francisco; Vioque, Javier

    2007-05-16

    Chickpea protein hydrolysates obtained with alcalase and flavourzyme were used for purification of copper chelating peptides by affinity chromatography using copper immobilized on solid supports. The chelating activity of purified peptides was indirectly measured by the inhibition of beta-carotene oxidation in the presence of copper. Two protein hydrolysates, obtained after 10 and 100 min of hydrolysis, were the most inhibitory of beta-carotene oxidation. Purified copper chelating peptides from these protein hydrolysates contained 19.7 and 35.1% histidine, respectively, in comparison to 2.7 and 2.6% in the protein hydrolysates. Chelating peptides from hydrolysate obtained after 10 min of hydrolysis were the most antioxidative being 8.3 times more antioxidative than the hydrolysate, while chelating peptides purified from protein hydrolysate obtained after 100 min were 3.1 times more antioxidative than its hydrolysate. However, the histidine content was higher in peptides derived from the 100 min hydrolysate (19.7 against 35.1% in 10 min hydrolysate), indicating that this amino acid is not the only factor involved in the antioxidative activity, and other factors such as peptide size or amino acid sequence are also determinant. This manuscript shows that affinity chromatography is a useful procedure for purification of copper chelating peptides. This method can be extended to other metals of interest in nutrition, such as calcium, iron, or zinc. Purified chelating peptides, in addition to their antioxidative properties, may also be useful in food mineral fortification for increasing the bioavailability of these metals.

  13. Relationship between oceanic heat content and sea surface height on interannual time scale

    Institute of Scientific and Technical Information of China (English)

    ZHANG Linlin; SUN Che; HU Dunxin

    2012-01-01

    The relationship between heat content and sea surface height (SSH) in the global ocean on the interannual time scale is examined with satellite altimeter measurements,historical hydrography,and model assimilation outputs.Results show that correlation between altimetric SSH and heat content in the upper 700 m calculated from Ishii data is geographically nonuniform.In the tropical ocean,heat content and SSH are strongly correlated and exhibit nearly the same interannual variations.In the polar ocean,their correlation is relatively weak.Further analysis with Simple Ocean Data Assimilation outputs shows that such nonuniform distribution is not from dynamical origin but from the limited integral depth selected to calculate heat content.The integral depth of 700 m is inadequate to capture variation of the deep main thermocline in the polar region.The halosteric effect also contributes to the nonuniform pattern of correlation,because saline contraction becomes significant in the polar ocean owing to low temperature.

  14. Optically stimulated phosphorescence in orthoclase feldspar over the millisecond to second time scale

    International Nuclear Information System (INIS)

    In the past, time-resolved IR stimulated luminescence (TR-IRSL) curves from feldspar have mainly been measured over a few hundred μs with the purpose of estimating the lifetimes of the components. In this study, we present the decay form of time-resolved IRSL and IR stimulated phosphorescence (IRSP) from orthoclase feldspar covering over 8 orders of magnitude (50 ns to ∼7 s). A detailed characterisation of the slowly decaying signals (ms to s time scales) from feldspar is undertaken to obtain further insight into the role of re-trapping in both the IR stimulated luminescence (IRSL) and the relatively more stable post-IR IRSL signals. The decay form of the different signals examined here shows a weak dependence on preheat temperature and a strong dependence on stimulation temperature. Interestingly, the IRSP curves show a conspicuous kink of which the position is linearly dependent on the on-time duration. The data on thermal dependence of these signals might suggest that the decay behaviour of the time-resolved IRSL and phosphorescence signals mainly reflect the occupancy of electrons in the band tail states with a significant contribution from the shallow traps. This interpretation is supported by thermoluminescence (TL) curves showing the photo-transfer effect during short IR and post-IR IR stimulations.

  15. Mixed-mode oscillations in a multiple time scale phantom bursting system

    CERN Document Server

    Krupa, Maciej; Desroches, Mathieu; Clément, Frédérique

    2012-01-01

    In this work we study mixed mode oscillations in a model of secretion of GnRH (Gonadotropin Releasing Hormone). The model is a phantom burster consisting of two feedforward coupled FitzHugh-Nagumo systems, with three time scales. The forcing system (Regulator) evolves on the slowest scale and acts by moving the slow nullcline of the forced system (Secretor). There are three modes of dynamics: pulsatility (transient relaxation oscillation), surge (quasi steady state) and small oscillations related to the passage of the slow nullcline through a fold point of the fast nullcline. We derive a variety of reductions, taking advantage of the mentioned features of the system. We obtain two results; one on the local dynamics near the fold in the parameter regime corresponding to the presence of small oscillations and the other on the global dynamics, more specifically on the existence of an attracting limit cycle. Our local result is a rigorous characterization of small canards and sectors of rotation in the case of fo...

  16. Time scales of regional circulation of saline fluids in continental aquifers (Armorican massif, Western France

    Directory of Open Access Journals (Sweden)

    A. Armandine Les Landes

    2014-06-01

    Full Text Available In recent decades, saline fluids have been sampled worldwide at great depths in continental basements. Although some of them have been attributed to marine transgressions the mechanisms allowing their circulation is not understood. In this paper, we describe the horizontal and vertical distribution of moderately saline fluids (60 to 1400 mg L−1 sampled at depths ranging from 41 to 200 m in aquifers at the regional scale of the Armorican Massif (northwestern France. The horizontal and vertical distributions of high chloride concentrations are in good agreement with both the altitudinal and vertical limits and succession of the three major transgressions between the Mio-Pliocene and Pleistocene ages. The mean chloride concentration for each transgression area is exponentially related to the time spanned until present. It defines the potential laws of leaching of marine waters by fresh meteoric waters. The results of the Armorican aquifers provide the first observed constraints for the time scales of seawater circulation in the continental basement and the subsequent leaching by fresh meteoric waters. The general trend of increasing chloride concentration with depth and the time frame for the flushing process provide useful information to develop conceptual models of the paleo-functionning of Armorican aquifers.

  17. Modeling and simulation of nuclear fuel in scenarios with long time scales

    Energy Technology Data Exchange (ETDEWEB)

    Espinosa, Carlos E.; Bodmann, Bardo E.J., E-mail: eduardo.espinosa@ufrgs.br, E-mail: bardo.bodmann@ufrgs.br [Universidade Federal do Rio Grande do Sul (DENUC/PROMEC/UFRGS), Porto Alegre, RS (Brazil). Departamento de Engenharia Nuclear. Programa de Pos Graduacao em Engenharia Mecanica

    2015-07-01

    Nuclear reactors play a key role in defining the energy matrix. A study by the Fraunhofer Society shows in different time scales for long periods of time the distribution of energy sources. Regardless of scale, the use of nuclear energy is practically constant. In these scenarios, the nuclear fuel behavior over time is of interest. For kinetics of long-term scales, changing the chemical composition of fuel is significant. Thus, it is appropriate to consider fission products called neutron poisons. Such products are of interest in the nuclear reactor, since they become parasitic neutron absorbers and result in long thermal heat sources. The objective of this work is to solve the kinetics system coupled to neutron poison products. To solve this system, we use similar ideas to the method of Adomian decomposition. Initially, one separates the system of equations as the sum of a linear part and a non-linear part in order to solve a recursive system. The nonlinearity is treated as Adomian polynomial. We present numerical results of the effects of changing the power of a reactor, scenarios such as start-up and shut-down. For these results we consider time dependent reactivity, such as linear reactivity, quadratic polynomial and oscillatory. With these results one can simulate the chemical composition of the fuel due to the reuse of the spent fuel in subsequent cycles. (author)

  18. Field Experience with and Potential for Multi-time Scale Grid Transactions from Responsive Commercial Buildings

    Energy Technology Data Exchange (ETDEWEB)

    Piette, Mary Ann; Kiliccote, Sila; Ghatikar, Girish

    2014-08-01

    The need for and concepts behind demand response are evolving. As the electric system changes with more intermittent renewable electric supply systems, there is a need to allow buildings to provide more flexible demand. This paper presents results from field studies and pilots, as well as engineering estimates of the potential capabilities of fast load responsiveness in commercial buildings. We present a sector wide analysis of flexible loads in commercial buildings, which was conducted to improve resource planning and determine which loads to evaluate in future demonstrations. These systems provide important capabilities for future transactional systems. The field analysis is based on results from California, plus projects in the northwest and east coast. End-uses considered include heating, ventilation, air conditioning and lighting. The timescales of control include day-ahead, as well as day-of, 10-minute ahead and even faster response. This technology can provide DR signals on different times scales to interact with responsive building loads. We describe the latency of the control systems in the building and the round trip communications with the wholesale grid operators.

  19. Saharan dust deposition may affect phytoplankton growth in the Mediterranean sea at ecological time scales.

    Directory of Open Access Journals (Sweden)

    Rachele Gallisai

    Full Text Available The surface waters of the Mediterranean Sea are extremely poor in the nutrients necessary for plankton growth. At the same time, the Mediterranean Sea borders with the largest and most active desert areas in the world and the atmosphere over the basin is subject to frequent injections of mineral dust particles. We describe statistical correlations between dust deposition over the Mediterranean Sea and surface chlorophyll concentrations at ecological time scales. Aerosol deposition of Saharan origin may explain 1 to 10% (average 5% of seasonally detrended chlorophyll variability in the low nutrient-low chlorophyll Mediterranean. Most of the statistically significant correlations are positive with main effects in spring over the Eastern and Central Mediterranean, conforming to a view of dust events fueling needed nutrients to the planktonic community. Some areas show negative effects of dust deposition on chlorophyll, coinciding with regions under a large influence of aerosols from European origin. The influence of dust deposition on chlorophyll dynamics may become larger in future scenarios of increased aridity and shallowing of the mixed layer.

  20. Application of matched filters in time-scale domain for elastography

    Institute of Scientific and Technical Information of China (English)

    ZHEN Bin; WAN Mingxi; SUN Yongmei

    2001-01-01

    Elasticity imaging offers the possibility of detecting changes in elastic properties and assesses the biomechanical properties of soft tissue with increased sensitivity and spatial resolution compared with conventional palpation. However, the range of applied strains is limited by the concomitant increase of echo signal decorrelation. The decorrelation is mainly introduced by diffuse scattering, while the regular scattering is highly correlated. Because the regular scattering and diffuse scattering localize with different patterns in different ranges of time-scale plane, a new method is put forward to detect the regular scattering with matched filters based on wavelet transform using Generalized Likelihood Ratio Test (GLRT). The simulation results illustrate that the change in estimated mean interscatterer spacing introduced by a SNR of -10 dB is 1.1 ± 2.8%. Thus, by tracking the highly correlated regular scattering,the internal strain can be estimated based on the change in interscatterer spacing under the condition of large surface deformation. The experiment studies show that the internal strain can be estimated up to 10% applied deformation in phantom and 5% strain in porcine liver.

  1. Discovery of a large time scale cyclic evolution of radio pulsars rotational frequency.

    Science.gov (United States)

    Beskin, G.; Biryukov, A.; Karpov, S.

    2006-08-01

    The recent massive measurements of pulsar frequency second derivatives have shown that they are 100-1000 times larger than expected for standard pulsar slowdown low. Moreover, the second derivatives as well as braking indices are even negative for about half of pulsars. We explain these paradoxical results on the basis of the statistical analysis of the rotational parameters (frequency, its first and second derivatives) of the subset of 295 pulsars taken mostly from the ATNF database. We have found strong correlation of second and first frequency derivatives either for positive (correlation coefficient r~0.9) and negative (r~0.85) values of second derivative, and of the frequency and and its first derivative (r~0.7). We interpret these dependencies as evolutionary ones due to the first frequency derivative being nearly proportional to the characteristic age. The derived statistical relations as well as "anomalous" values of the second frequency derivative are well explained in the framework of the simple model of cyclic evolution of the rotational frequency of the pulsars. It combines the secular change of the rotational parameters according to the power law with braking index n~5 and harmonic oscillations of 100--1000 years period with an amplitude from 10^-3 Hz for young pulsars to 10^-10 Hz for elder ones. The physical nature of these cyclic variations of the rotational frequency may be similar to the well-known red timing noise, however, with much larger characteristic time scale.

  2. Asymptotic self-similar solutions with a characteristic time-scale

    CERN Document Server

    Waxman, Eli

    2010-01-01

    For a wide variety of initial and boundary conditions, adiabatic one dimensional flows of an ideal gas approach self-similar behavior when the characteristic length scale over which the flow takes place, $R$, diverges or tends to zero. It is commonly assumed that self-similarity is approached since in the $R\\to\\infty(0)$ limit the flow becomes independent of any characteristic length or time scales. In this case the flow fields $f(r,t)$ must be of the form $f(r,t)=t^{\\alpha_f}F(r/R)$ with $R\\propto(\\pm t)^\\alpha$. We show that requiring the asymptotic flow to be independent only of characteristic length scales imply a more general form of self-similar solutions, $f(r,t)=R^{\\delta_f}F(r/R)$ with $\\dot{R}\\propto R^\\delta$, which includes the exponential ($\\delta=1$) solutions, $R\\propto e^{t/\\tau}$. We demonstrate that the latter, less restrictive, requirement is the physically relevant one by showing that the asymptotic behavior of accelerating blast-waves, driven by the release of energy at the center of a co...

  3. A SKOS-based multilingual thesaurus of geological time scale for interoperability of online geological maps

    Science.gov (United States)

    Ma, Xiaogang; Carranza, Emmanuel John M.; Wu, Chonglong; van der Meer, Freek D.; Liu, Gang

    2011-10-01

    The usefulness of online geological maps is hindered by linguistic barriers. Multilingual geoscience thesauri alleviate linguistic barriers of geological maps. However, the benefits of multilingual geoscience thesauri for online geological maps are less studied. In this regard, we developed a multilingual thesaurus of geological time scale (GTS) to alleviate linguistic barriers of GTS records among online geological maps. We extended the Simple Knowledge Organization System (SKOS) model to represent the ordinal hierarchical structure of GTS terms. We collected GTS terms in seven languages and encoded them into a thesaurus by using the extended SKOS model. We implemented methods of characteristic-oriented term retrieval in JavaScript programs for accessing Web Map Services (WMS), recognizing GTS terms, and making translations. With the developed thesaurus and programs, we set up a pilot system to test recognitions and translations of GTS terms in online geological maps. Results of this pilot system proved the accuracy of the developed thesaurus and the functionality of the developed programs. Therefore, with proper deployments, SKOS-based multilingual geoscience thesauri can be functional for alleviating linguistic barriers among online geological maps and, thus, improving their interoperability.

  4. Analysis and modelling of variability and covariability of population spike trains across multiple time scales.

    Science.gov (United States)

    Lyamzin, Dmitry R; Garcia-Lazaro, Jose A; Lesica, Nicholas A

    2012-01-01

    As multi-electrode and imaging technology begin to provide us with simultaneous recordings of large neuronal populations, new methods for modelling such data must also be developed. We present a model of responses to repeated trials of a sensory stimulus based on thresholded Gaussian processes that allows for analysis and modelling of variability and covariability of population spike trains across multiple time scales. The model framework can be used to specify the values of many different variability measures including spike timing precision across trials, coefficient of variation of the interspike interval distribution, and Fano factor of spike counts for individual neurons, as well as signal and noise correlations and correlations of spike counts across multiple neurons. Using both simulated data and data from different stages of the mammalian auditory pathway, we demonstrate the range of possible independent manipulations of different variability measures, and explore how this range depends on the sensory stimulus. The model provides a powerful framework for the study of experimental and surrogate data and for analyzing dependencies between different statistical properties of neuronal populations. PMID:22578115

  5. US stock market efficiency over weekly, monthly, quarterly and yearly time scales

    Science.gov (United States)

    Rodriguez, E.; Aguilar-Cornejo, M.; Femat, R.; Alvarez-Ramirez, J.

    2014-11-01

    In financial markets, the weak form of the efficient market hypothesis implies that price returns are serially uncorrelated sequences. In other words, prices should follow a random walk behavior. Recent developments in evolutionary economic theory (Lo, 2004) have tailored the concept of adaptive market hypothesis (AMH) by proposing that market efficiency is not an all-or-none concept, but rather market efficiency is a characteristic that varies continuously over time and across markets. Within the AMH framework, this work considers the Dow Jones Index Average (DJIA) for studying the deviations from the random walk behavior over time. It is found that the market efficiency also varies over different time scales, from weeks to years. The well-known detrended fluctuation analysis was used for the characterization of the serial correlations of the return sequences. The results from the empirical showed that interday and intraday returns are more serially correlated than overnight returns. Also, some insights in the presence of business cycles (e.g., Juglar and Kuznets) are provided in terms of time variations of the scaling exponent.

  6. Mastering Uncertainty and Risk at Multiple Time Scales in the Future Electrical Grid

    Energy Technology Data Exchange (ETDEWEB)

    Chertkov, Michael [Los Alamos National Laboratory; Bent, Russell W. [Los Alamos National Laboratory; Backhaus, Scott N. [Los Alamos National Laboratory

    2012-07-10

    Today's electrical grids enjoy a relatively clean separation of spatio-temporal scales yielding a compartmentalization of grid design, optimization, control and risk assessment allowing for the use of conventional mathematical tools within each area. In contrast, the future grid will incorporate time-intermittent renewable generation, operate via faster electrical markets, and tap the latent control capability at finer grid modeling scales; creating a fundamentally new set of couplings across spatiotemporal scales and requiring revolutionary advances in mathematics techniques to bridge these scales. One example is found in decade-scale grid expansion planning in which today's algorithms assume accurate load forecasts and well-controlled generation. Incorporating intermittent renewable generation creates fluctuating network flows at the hourly time scale, inherently linking the ability of a transmission line to deliver electrical power to hourly operational decisions. New operations-based planning algorithms are required, creating new mathematical challenges. Spatio-temporal scales are also crossed when the future grid's minute-scale fluctuations in network flows (due to intermittent generation) create a disordered state upon which second-scale transient grid dynamics propagate effectively invalidating today's on-line dynamic stability analyses. Addressing this challenge requires new on-line algorithms that use large data streams from new grid sensing technologies to physically aggregate across many spatial scales to create responsive, data-driven dynamic models. Here, we sketch the mathematical foundations of these problems and potential solutions.

  7. SCALABLE PERCEPTUAL AUDIO REPRESENTATION WITH AN ADAPTIVE THREE TIME-SCALE SINUSOIDAL SIGNAL MODEL

    Institute of Scientific and Technical Information of China (English)

    Al-Moussawy Raed; Yin Junxun; Song Shaopeng

    2004-01-01

    This work is concerned with the development and optimization of a signal model for scalable perceptual audio coding at low bit rates. A complementary two-part signal model consisting of Sines plus Noise (SN) is described. The paper presents essentially a fundamental enhancement to the sinusoidal modeling component. The enhancement involves an audio signal scheme based on carrying out overlap-add sinusoidal modeling at three successive time scales,large, medium, and small. The sinusoidal modeling is done in an analysis-by-synthesis overlapadd manner across the three scales by using a psychoacoustically weighted matching pursuits.The sinusoidal modeling residual at the first scale is passed to the smaller scales to allow for the modeling of various signal features at appropriate resolutions. This approach greatly helps to correct the pre-echo inherent in the sinusoidal model. This improves the perceptual audio quality upon our previous work of sinusoidal modeling while using the same number of sinusoids. The most obvious application for the SN model is in scalable, high fidelity audio coding and signal modification.

  8. Asymptotic Convergence of the Solutions of a Dynamic Equation on Discrete Time Scales

    Directory of Open Access Journals (Sweden)

    J. Diblík

    2012-01-01

    Full Text Available The paper investigates a dynamic equation Δy(tn=β(tn[y(tn−j−y(tn−k] for n→∞, where k and j are integers such that k>j≥0, on an arbitrary discrete time scale T:={tn} with tn∈ℝ, n∈ℤn0−k∞={n0−k,n0−k+1,…}, n0∈ℕ, tn

  9. Atomistic surface erosion and thin film growth modelled over realistic time scales

    International Nuclear Information System (INIS)

    We present results of atomistic modelling of surface growth and sputtering using a multi-time scale molecular dynamics-on-the-fly kinetic Monte Carlo scheme which allows simulations to be carried out over realistic experimental times. The method uses molecular dynamics to model the fast processes and then calculates the diffusion barriers for the slow processes on-the-fly, without any preconceptions about what transitions might occur. The method is applied to the growth of metal and oxide materials at impact energies typical for both vapour deposition and magnetron sputtering. The method can be used to explain growth processes, such as the filling of vacancies and the formation of stacking faults. By tuning the variable experimental parameters on the computer, a parameter set for optimum crystalline growth can be determined. The method can also be used to model sputtering where the particle interactions with the surface occur at a higher energy. It is shown how a steady state can arise in which interstitial clusters are continuously being formed below the surface during an atom impact event which also recombine or diffuse to the surface between impact events. For fcc metals the near surface region remains basically crystalline during the erosion process with a pitted topography which soon attains a steady state roughness.

  10. Southern Ocean SST Variability and Its Relationship with ENSO on Inter-Decadal Time Scales

    Institute of Scientific and Technical Information of China (English)

    YAN Li; DU Yan; ZHANG Lan

    2013-01-01

    Empirical orthogonal function (EOF) analysis reveals a co-variability of Sea surface temperatures (SSTs) in the Southern Hemisphere (0°-60°S).In the South Indian and Atlantic Oceans,there is a subtropical dipole pattern slanted in the southwest-northeast direction.In the South Pacific Ocean,a meridional tripole structure emerges,whose middle pole co-varies with the dipoles in the South Indian and Atlantic Oceans and is used in this study to track subtropical Pacific variability.The South Indian and Atlantic Ocean dipoles and the subtropical Pacific variability are phase-locked in austral summer.On the inter-decadal time scales,the dipoles in the South Indian and Atlantic Oceans weaken in amplitude after 1979/1980.No such weakening is found in the subtropical South Pacific Ocean.Interestingly,despite the reduced amplitude,the correlation of the Indian Ocean and Atlantic dipoles with El Ni(n)o and Southern Oscillation (ENSO) are enhanced after 1979/1980.The same increase in correlation is found for subtropical South Pacific variability after 1979/1980.These inter-decadal modulations imply that the Southem Hemisphere participates in part of the climate shift in the late 1970s.The correlation between Southern Hemisphere SST and ENSO reduces after 2000.

  11. The time scales of the climate-economy feedback and the climatic cost of growth

    Energy Technology Data Exchange (ETDEWEB)

    Hallegatte, Stephane [CIRED - CNRM, Nogent-sur-Marne (France)

    2005-04-01

    This paper is based on the perception that the inertia of climate and socio-economic systems are key parameters in the climate change issue. In a first part, it develops and implements a new approach based on a simple integrated model with a particular focus on an innovative transient impact and adaptation modelling. In a second part, a climate-economy feedback is defined and characterized. It is found that: (i) it has a 70-year characteristic time, which is long when compared to the system's other time-scales, and it cannot act as a natural damping process of climate change; (ii) mitigation has to be anticipated since the feedback of an emission reduction on the economy is significant only after a 20-year delay and really efficient after a one-century delay; (iii) the IPCC methodology, that neglects the feedback from impacts to emissions, is acceptable up to 2100, whatever is the level of impacts. This analysis allows also to define a climatic cost of growth as the additional climate change damages due to the additional emissions linked to economic growth. Usefully, this metric for climate change damages is particularly independent of the baseline scenario. (orig.)

  12. Minimum spanning tree filtering of correlations for varying time scales and size of fluctuations

    CERN Document Server

    Kwapien, Jaroslaw; Forczek, Marcin; Drozdz, Stanislaw

    2016-01-01

    Based on a recently proposed $q$-dependent detrended cross-correlation coefficient $\\rho_q$ (J.~Kwapie\\'n, P.~O\\'swi\\k{e}cimka, S.~Dro\\.zd\\.z, Phys. Rev.~E 92, 052815 (2015)), we introduce a family of $q$-dependent minimum spanning trees ($q$MST) that are selective to cross-correlations between different fluctuation amplitudes and different time scales of multivariate data. They inherit this ability directly from the coefficients $\\rho_q$ that are processed here to construct a distance matrix being the input to the MST-constructing Kruskal's algorithm. In order to illustrate their performance, we apply the $q$MSTs to sample empirical data from the American stock market and discuss the results. We show that the $q$MST graphs can complement $\\rho_q$ in detection of "hidden" correlations that cannot be observed by the MST graphs based on $\\rho_{\\rm DCCA}$ and, therefore, they can be useful in many areas where the multivariate cross-correlations are of interest (e.g., in portfolio analysis).

  13. Evaluating the uncertainty of predicting future climate time series at the hourly time scale

    Science.gov (United States)

    Caporali, E.; Fatichi, S.; Ivanov, V. Y.

    2011-12-01

    A stochastic downscaling methodology is developed to generate hourly, point-scale time series for several meteorological variables, such as precipitation, cloud cover, shortwave radiation, air temperature, relative humidity, wind speed, and atmospheric pressure. The methodology uses multi-model General Circulation Model (GCM) realizations and an hourly weather generator, AWE-GEN. Probabilistic descriptions of factors of change (a measure of climate change with respect to historic conditions) are computed for several climate statistics and different aggregation times using a Bayesian approach that weights the individual GCM contributions. The Monte Carlo method is applied to sample the factors of change from their respective distributions thereby permitting the generation of time series in an ensemble fashion, which reflects the uncertainty of climate projections of future as well as the uncertainty of the downscaling procedure. Applications of the methodology and probabilistic expressions of certainty in reproducing future climates for the periods, 2000 - 2009, 2046 - 2065 and 2081 - 2100, using the 1962 - 1992 period as the baseline, are discussed for the location of Firenze (Italy). The climate predictions for the period of 2000 - 2009 are tested against observations permitting to assess the reliability and uncertainties of the methodology in reproducing statistics of meteorological variables at different time scales.

  14. Determination of peptide and protein diversity in venom of the spider Selenocosmia jiafu by high performance liquid chromatography and mass spectrometry%采用高效液相色谱-质谱考察家福捕鸟蛛粗毒中多肽和蛋白质的多样性

    Institute of Scientific and Technical Information of China (English)

    胡朝暾; 肖震; 周熙; 陈佳; 陈波; 刘中华

    2015-01-01

    solution B( acetonitrile with 0. 1% trifluoroacetic acid)over 50 min with a flow rate of 1 mL/min. The isolated venom proteins were treated with in-gel digestion separated by SDS-PAGE and then identified by liquid chromatography-electrospray ionization quadrupole-time of flight mass spectrometry( LC-ESI-QTOF-MS)techniques. The results show that more than 40 fractions eluted were monitored at 215 nm in the RP-HPLC chromatogram of the venom of the spider S. jiafu. Most of the fractions were eluted with retention times of 5-15 min and 25-40 min,corresponding to 5% -15% and 25% -40% acetonitrile,respectively. The venom contains 238 peptides that follow a bimodal distribution,with about 62. 5% of the peptides having a rela-tive molecular mass of 3 000-4 500 and about 33. 2% of the peptides having a relative molecular mass of 1 000-3 000. This distribution model is rather different from those of peptides from oth-er tarantula spider venoms analyzed. To explore the relative molecular mass distribution of the venom proteins,the venom was analyzed by SDS-PAGE using standard protocols. Except for peptides with relative molecular mass lower than 10 000, the SDS-PAGE electrophoresis revealed three more obvious bands around 50,72 and 90 kD respectively. Further MS analysis indicated that there are mainly hemocyanin,potassium ion channel protein,calcium protease and so on. Altogether,this study not only indicated there are many peptides and proteins in the S. jiafu venom,but also provided a basis for further case-by-case investigation of peptide tox-ins from this venom.

  15. High-performance liquid chromatography of biopolymers

    Energy Technology Data Exchange (ETDEWEB)

    Regnier, F.E.

    1983-10-21

    The ability to separate biological macromolecules with good resolution on liquid chromatographic columns has depended on the development of suitable packing materials. In size exclusion chromatography, molecules are separated by size on the basis of differential permeation of the packing. Ion exchange, hydrophobic interaction (or reversed-phase), and affinity chromatography are all surface-mediated separation methods, although they depend on different retention mechanisms. High-performance liquid chromatographic columns designed for biopolymers offer major advantages over conventional columns in both speed and resolving power. The exponential growth of literature on the high-performance separation of peptides and proteins in particular indicates that the techniques will become the dominant form of column liquid chromatography. 92 refs., 4 figs.

  16. Biosynthetic engineering of nonribosomal peptide synthetases.

    Science.gov (United States)

    Kries, Hajo

    2016-09-01

    From the evolutionary melting pot of natural product synthetase genes, microorganisms elicit antibiotics, communication tools, and iron scavengers. Chemical biologists manipulate these genes to recreate similarly diverse and potent biological activities not on evolutionary time scales but within months. Enzyme engineering has progressed considerably in recent years and offers new screening, modelling, and design tools for natural product designers. Here, recent advances in enzyme engineering and their application to nonribosomal peptide synthetases are reviewed. Among the nonribosomal peptides that have been subjected to biosynthetic engineering are the antibiotics daptomycin, calcium-dependent antibiotic, and gramicidin S. With these peptides, incorporation of unnatural building blocks and modulation of bioactivities via various structural modifications have been successfully demonstrated. Natural product engineering on the biosynthetic level is not a reliable method yet. However, progress in the understanding and manipulation of biosynthetic pathways may enable the routine production of optimized peptide drugs in the near future. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd. PMID:27465074

  17. Ecotonal Control on Vadose-Zone Fluxes in Arid Regions Over Very Long Time Scales

    Science.gov (United States)

    Phillips, F. M.; Walvoord, M. A.; Sandvig, R.

    2003-12-01

    Recent studies indicate that vegetation plays an important role in regulating recharge in semiarid and arid basins over very long time scales. Several lines of evidence from desert floor environments in the southwestern United States suggest that vegetation has established essentially permanent upward hydraulic gradients, effectively precluding diffuse recharge since the transition from woodland to xeric scrub in the early Holocene. However, less xeric vegetation (such as the pygmy piñon and juniper forest) may support significant diffuse recharge. We show comparative water potential and porewater chemistry profiles collected from various vegetation communities in the Chihuahuan Desert of west Texas. The modeled soil water (vapor and liquid) flux regimes illustrate a conversion from substantial downward fluxes under the mixed woodland to upward fluxes under grassland and xeric scrub. Model results also indicated a trend in increasing drying front propagation depth from the grassland to recently-encroached xeric scrub to well-established xeric scrub. Drying fronts are the result of upward soil water fluxes initiated up to 16 thousand years ago in the xeric scrub community. In contrast, the nearby woodland community supports active, and likely episodic, recharge on the order of 5 to 15 mm yr-1. The mechanism by which some vegetation takes up essentially all seasonally available moisture within the root zone, preventing downward soil water fluxes for periods of thousands of years, but adjacent vegetation communities regularly permit downward fluxes, remains to be determined. Nevertheless, these results suggest that understanding the relation between vegetation community and vadose-zone hydrological processes may be the most profitable avenue toward quantifying diffuse groundwater recharge. We hypothesize that vegetation type may be a reasonable proxy for estimating recharge in semiarid and arid basins. Ongoing research is intended to test the hypothesis of ecotonal

  18. Determination of paleoseismic activity over a large time-scale: Fault scarp dating with 36Cl

    Science.gov (United States)

    Mozafari Amiri, Nasim; Tikhomirov, Dmitry; Sümer, Ökmen; Özkaymak, Çaǧlar; Uzel, Bora; Ivy-Ochs, Susan; Vockenhuber, Christof; Sözbilir, Hasan; Akçar, Naki

    2016-04-01

    Bedrock fault scarps are the most direct evidence of past earthquakes to reconstruct seismic activity in a large time-scale using cosmogenic 36Cl dating if built in carbonates. For this method, a surface along the fault scarp with a minimum amount of erosion is required to be chosen as an ideal target point. The section of the fault selected for sampling should cover at least two meters of the fault surface from the lower part of the scarp, where intersects with colluvium wedge. Ideally, sampling should be performed on a continuous strip along the direction of the fault slip direction. First, samples of 10 cm high and 15 cm wide are marked on the fault surface. Then, they are collected using cutters, hammer and chisel in a thickness of 3 cm. The main geometrical factors of scarp dip, scarp height, top surface dip and colluvium dip are also measured. Topographic shielding in the sampling spot is important to be estimated as well. Moreover, density of the fault scarp and colluvium are calculated. The physical and chemical preparations are carried in laboratory for AMS and chemical analysis of the samples. A Matlab® code is used for modelling of seismically active periods based on increasing production rate of 36Cl following each rupture, when a buried section of a fault is exposed. Therefore, by measuring the amount of cosmogenic 36Cl versus height, the timing of major ruptures and their offsets are determined. In our study, Manastır, Mugırtepe and Rahmiye faults in Gediz graben, Priene-Sazlı, Kalafat and Yavansu faults in Büyük Menderes graben and Ören fault in Gökava half-graben have been examined in the seismically active region of Western Turkey. Our results reconstruct at least five periods of high seismic activity during the Holocene time, three of which reveal seismic ruptures beyond the historical pre-existing data.

  19. Revealing the aerosol radiative impact of volcanic ash on synoptic time scales

    Science.gov (United States)

    Walter, Carolin; Rieger, Daniel; Gasch, Philipp; Förstner, Jochen; Vogel, Bernhard

    2016-04-01

    Including the interactions of aerosols with radiation in weather forecast models often leads to perturbations of the temperature field even at locations not directly influenced by the regarded aerosols. They arise out of signals propagating with the speed of sound leading to abrupt changes in cloud cover. The temperature perturbations due to these changes hamper the quantification of the aerosol radiative impact as they can appear in the same order of magnitude. In order to reveal the aerosol radiative impact on synoptic time scales we introduce a new method to separate the aerosol induced temperature effect from atmospheric perturbations. We simulated the impact of volcanic ash aerosol on radiation with the new global to regional scale modelling system ICON-ART (ICOsahedral Nonhydrostatic - Aerosols and Reactive Trace gases; Rieger et al., 2015). Within ICON-ART the radiative fluxes and cooling rates are calculated with the RRTM (Rapid Radiative Transfer Model; Mlawer et al., 1997) for 30 longwave and shortwave bands. To determine the optical properties of the prognostic ash aerosol, Mie calculations were conducted for a compilation of ash refractive indices. We obtain a significant change in 2 m temperature of up to several Kelvin for the Puyehue-Cordon Caulle eruption in 2011. In addition to the temperature effect the atmospheric stability is modified and as a consequence the ash concentrations. The temperature effect during the Eyjafjallajökull eruption in 2010 over Europe is much less pronounced. Nevertheless, we are able to show the impact of volcanic ash on the state of the atmosphere by this eruption.

  20. Synchrony between reanalysis-driven RCM simulations and observations: variation with time scale

    Science.gov (United States)

    de Elía, Ramón; Laprise, René; Biner, Sébastien; Merleau, James

    2016-06-01

    Unlike coupled global climate models (CGCMs) that run in a stand-alone mode, nested regional climate models (RCMs) are driven by either a CGCM or a reanalysis dataset. This feature makes high correlations between the RCM simulation and its driver possible. When the driving dataset is a reanalysis, time correlations between RCM output and observations are also common and to be expected. In certain situations time correlation between driver and driven RCM is of particular interest and techniques have been developed to increase it (e.g. large-scale spectral nudging). For such cases, a question that remains open is whether aggregating in time increases the correlation between RCM output and observations. That is, although the RCM may be unable to reproduce a given daily event, whether it will still be able to satisfactorily simulate an anomaly on a monthly or annual basis. This is a preconception that the authors of this work and others in the community have held, perhaps as a natural extension of the properties of upscaling or aggregating other statistics such as the mean squared error. Here we explore analytically four particular cases that help us partially answer this question. In addition, we use observations datasets and RCM-simulated data to illustrate our findings. Results indicate that time upscaling does not necessarily increase time correlations, and that those interested in achieving high monthly or annual time correlations between RCM output and observations may have to do so by increasing correlation as much as possible at the shortest time scale. This may indicate that even when only concerned with time correlations at large temporal scale, large-scale spectral nudging acting at the time-step level may have to be used.

  1. The plumbing of the global biological pump: Efficiency control through leaks, pathways, and time scales

    Science.gov (United States)

    Pasquier, Benoît; Holzer, Mark

    2016-08-01

    We systematically quantify the pathways and time scales that set the efficiency, Ebio, of the global biological pump by applying Green-function-based diagnostics to a data-assimilated phosphorus cycle embedded in a jointly assimilated ocean circulation. We consider "bio pipes" that consist of phosphorus paths that connect specified regions of last biological utilization with regions where regenerated phosphate first reemerges into the euphotic zone. The bio pipes that contribute most to Ebio connect the Eastern Equatorial Pacific (EEqP) and Equatorial Atlantic to the Southern Ocean ((21 ± 3)% of Ebio), as well as the Southern Ocean to itself ((15 ± 3)% of Ebio). The bio pipes with the largest phosphorus flow rates connect the EEqP to itself and the subantarctic Southern Ocean to itself. The global mean sequestration time of the biological pump is 130 ± 70 years, while the sequestration time of the bio pipe from anywhere to the Antarctic region of the Southern Ocean is 430 ± 30 years. The distribution of phosphorus flowing within a given bio pipe is quantified by its transit-time partitioned path density. For the largest bio pipes, ˜1/7 of their phosphorus is carried by thermocline paths with transit times less than ˜300-400 years, while ˜4/7 of their phosphorus is carried by abyssal paths with transit times exceeding ˜700 years. The path density reveals that Antarctic Intermediate Water carries about a third of the regenerated phosphate last utilized in the EEqP that is destined for the Southern Ocean euphotic zone. The Southern Ocean is where (62 ± 2)% of the regenerated inventory and (69 ± 1)% of the preformed inventory first reemerge into the euphotic zone.

  2. A short-time scale colloidal system reveals early bacterial adhesion dynamics.

    Directory of Open Access Journals (Sweden)

    Christophe Beloin

    2008-07-01

    Full Text Available The development of bacteria on abiotic surfaces has important public health and sanitary consequences. However, despite several decades of study of bacterial adhesion to inert surfaces, the biophysical mechanisms governing this process remain poorly understood, due, in particular, to the lack of methodologies covering the appropriate time scale. Using micrometric colloidal surface particles and flow cytometry analysis, we developed a rapid multiparametric approach to studying early events in adhesion of the bacterium Escherichia coli. This approach simultaneously describes the kinetics and amplitude of early steps in adhesion, changes in physicochemical surface properties within the first few seconds of adhesion, and the self-association state of attached and free-floating cells. Examination of the role of three well-characterized E. coli surface adhesion factors upon attachment to colloidal surfaces--curli fimbriae, F-conjugative pilus, and Ag43 adhesin--showed clear-cut differences in the very initial phases of surface colonization for cell-bearing surface structures, all known to promote biofilm development. Our multiparametric analysis revealed a correlation in the adhesion phase with cell-to-cell aggregation properties and demonstrated that this phenomenon amplified surface colonization once initial cell-surface attachment was achieved. Monitoring of real-time physico-chemical particle surface properties showed that surface-active molecules of bacterial origin quickly modified surface properties, providing new insight into the intricate relations connecting abiotic surface physicochemical properties and bacterial adhesion. Hence, the biophysical analytical method described here provides a new and relevant approach to quantitatively and kinetically investigating bacterial adhesion and biofilm development.

  3. Nitrogen Cycling in the Black Sea on Glacial-Interglacial Time Scales

    Science.gov (United States)

    Quan, T. M.; Wright, J. D.; Falkowski, P. G.

    2010-12-01

    On geological time scales, the nitrogen cycle consists of three main processes: nitrogen fixation, nitrification, and denitrification/anammox, all of which are affected by the amount of oxygen present in the water column. The balance between nitrogen fixation and denitrification/anammox controls the pool of fixed inorganic nitrogen, and can be traced using nitrogen isotopes. We have previously proposed a conceptual model that relates sedimentary δ15N to deep water oxygen concentrations using a simple, non-linear curve. Provided that the sediment record is well-preserved, the nitrogen isotopic profile at a given location reflects history of the relative contributions of nitrogen fixation to denitrifiation and thus the past redox environment. Our goal was to test this model using deep sea sediments from the Black Sea, which alternates from an anoxic, stratified, marine water body during interglacial periods to a less saline, oxygenated lake during glacial events. We find that the measured δ15N profile in general corresponds to the changes in redox state as determined by trace metal concentrations and micropaleontology. Higher δ15N values occur during suboxic transitional periods, with lower δ15N values appearing under anoxic and oxic conditions, supporting our hypothesis that the nitrogen cycle is regulated by oxygen concentration. We also identify some intervals where the correlation between redox proxies appears to fail. While the lack of ancillary data from the paleo-Black Sea makes interpretation difficult, we believe analysis of these intervals may provide more information about Black Sea paleoenvironmental and paleoredox conditions.

  4. What is the Time Scale for Orbital Forcing of the Martian Water Cycle?

    Science.gov (United States)

    Hecht, M. H.

    2003-01-01

    Calculation of the periodic variations in the martian orbital parameters by Ward and subsequent refinements to the theory have inspired numerous models of variation of the martian water cycle. Most of these models have focused on variations in planetary obliquity on a both a short-term (110 kyr) time scale as well as larger oscillations occuring over millions of years. To a lesser extent, variations in planetary eccentricity have also been considered. The third and fastest mode of variation, the precession of the longitude of perihelion, has generally been deemphasized because, among the three parameters, it is the only one that does not change the integrated annual insolation. But as a result of this precession, the asymmetry in peak summer insolation between the poles exceeds 50%, with the maximum cycling between poles every 25.5 kyrs. The relative contribution of these different elements to orbital forcing of climate takes on particular importance in the context of apparently recent waterrelated features such as gullies or polar layered deposits (PLD). Christensen, for example, recently indentified mantling of heavily gullied crater walls as residual dust-covered snow deposits that were responsible for the formation of the gullies in a previous epoch. Christensen assumed that the snow was originally deposited at a period of high obliquity which was stabilized against sublimation by a lag deposit of dust. It is suggested here that not obliquity, but the shortterm oscillations associated with precession of the perihelion may play the dominant role in the formation of gullies, major strata in the polar layered deposits (PLD), and other water-related features.

  5. Ventilation of the Black Sea pycnocline on seasonal and interannual time scales

    Directory of Open Access Journals (Sweden)

    L.I. IVANOV

    2012-12-01

    Full Text Available The paper is a description of temporal variability of winter cooling conditions and estimation of effective cross-isopycnal mixing rates in the Black Sea. Data averaging versus salinity / sigma-t scale was used to filter effects of local dynamics. It is shown that traces of winter mixing events appear well preserved in the temperature-salinity structure, due to the peculiarities of the Black Sea where temperature often acts as a passive tracer with a smaller contribution to density as compared to salinity. Vertical distribution of the magnitudes of temperature oscillations indicates that the convection events have limited effects in modifying the structure of the middle and lower pycnocline on a seasonal time scale. However, long-term fluctuations are well recognised. The magnitudes of the seasonal and long-term temperature fluctuations are comparable only in the upper pycnocline. Three major cooling events can be distinguished from the record of the pycnocline temperature for the past 75 years. The intensive cooling occurred in the late 1920s - early 1930s, early 1950s and late 1980s - early 1990s. Partial renewal of the water of the cold intermediate layer core took place approximately once in two years. The period when convection causes erosion of the pycnocline lasts for only a week. It is shown that a lateral source of heat and salt exists for the upper pycnocline, where it is the cold intermediate water, and for the lower pycnocline, the layer below S@ 20.5, where this lateral source of salt and heat is maintained by disintegrating Bosphorus plume.

  6. Estimating the flood frequency distribution at seasonal and annual time scales

    Directory of Open Access Journals (Sweden)

    E. Baratti

    2012-12-01

    Full Text Available We propose an original approach to infer the flood frequency distribution at seasonal and annual time scale. Our purpose is to estimate the peak flow that is expected for an assigned return period T, independently of the season in which it occurs (i.e. annual flood frequency regime, as well as in different selected sub-yearly periods (i.e. seasonal flood frequency regime. While a huge literature exists on annual flood frequency analysis, few studies have focused on the estimation of seasonal flood frequencies despite the relevance of the issue, for instance when scheduling along the months of the year the construction phases of river engineering works directly interacting with the active river bed, like for instance dams. An approximate method for joint frequency analysis is presented here that guarantees consistency between fitted annual and seasonal distributions, i.e. the annual cumulative distribution is the product of the seasonal cumulative distribution functions, under the assumption of independence among floods in different seasons. In our method the parameters of the seasonal frequency distributions are fitted by maximising an objective function that accounts for the likelihoods of both seasonal and annual peaks. In contrast to previous studies, our procedure is conceived to allow the users to introduce subjective weights to the components of the objective function in order to emphasize the fitting of specific seasons or of the annual peak flow distribution. An application to the time series of the Blue Nile daily flows at the Sudan–Ethiopia border is presented.

  7. "Tectonic Petrameter," An Alternative Method to Teaching the Geologic Time Scale

    Science.gov (United States)

    Posner, E. S.

    2011-12-01

    I have over a decade of experience as a performance poet and am now a graduate student in the geosciences. I have created a performance poem / play script, "Tectonic Petrameter," as an alternative method of teaching the geologic time scale. "The Archean came next and it was a blast. Tectonic plates were smaller and they moved pretty fast. In an enthusiastic flash of ash, volcanic islands smashed together." The use of rhyme and rhythm presents a different and interdisciplinary approach to teaching Earth history that appeals to a wide range of learning styles and makes science fun, while clearly describing important concepts in geology and events in Earth history. "Now it's time to get down with the Coal Swamp Stomp! Tap your feet to the beat of the formation of peat like a plant plantation soaking up the bright heat." "Tectonic Petrameter" by itself is an illustrated spoken-word poem that leads audiences from all levels of scientific background on an excitingly educational journey through geologic time. I will perform my 10-minute memorized poem and present results from my ongoing study to assess the effectiveness of "Tectonic Petrameter" as a teaching tool in K-12 and introductory undergraduate classroom curricula. I propose that using "Tectonic Petrameter" as a performance piece and theatrical play script in K-12 and introductory undergraduate classrooms, as well as in broader community venues, may be an avenue for breaking down barriers related to teaching about Earth's long and complex history. Digital copies of "Tectonic Petrameter" will be made available to interested parties.

  8. Estimating the flood frequency distribution at seasonal and annual time scale

    Directory of Open Access Journals (Sweden)

    E. Baratti

    2012-06-01

    Full Text Available We propose an original approach to infer the flood frequency distribution at seasonal and annual time scale. Our purpose is to estimate the peak flow that is expected for an assigned return period T, independently of the season in which it occurs (i.e. annual flood frequency regime, as well as in different selected sub-yearly periods (i.e. seasonal flood frequency regime. While a huge literature exists on annual flood frequency analysis, few studies have focused on the estimation of seasonal flood frequencies despite the relevance of the issue, for instance when scheduling along the months of the year the construction phases of river engineering works directly interacting with the active river bed, like for instance dams. An approximate method for joint frequency analysis is presented here that guarantees consistency between fitted annual and seasonal distributions, i.e. the annual cumulative distribution is the product of the seasonal cumulative distribution functions, under the assumption of independence among floods in different seasons. In our method the parameters of the seasonal frequency distributions are fitted by maximising an objective function that accounts for the likelihoods of both seasonal and annual peaks. Differently from previous studies, our procedure is conceived to allow the users to introduce subjective weights to the components of the objective function in order to emphasize the fitting of specific seasons or of the annual peak flow distribution. An application to the time series of the Blue Nile daily flows at Sudan-Ethiopia border is presented.

  9. A Time-scale Decomposed Threshold Regression Downscaling Approach to Forecasting South China Early Summer Rainfall

    Science.gov (United States)

    Song, Linye; Duan, Wansuo; Li, Yun; Mao, Jiangyu

    2015-04-01

    A time-scale decomposed threshold regression (TSDTR) downscaling approach to forecasting South China early summer rainfall (SCESR) is described by using long-term observed station rainfall data and the National Oceanic and Atmospheric Administration Extended Reconstructed sea surface temperature (SST) data. It makes use of two distinct regression downscaling models corresponding to the interannual and interdecadal rainfall variability of SCESR. The two models were developed based on the partial least square (PLS) regression technique linking SCESR to SST modes in preceding months on both interannual and interdecadal timescales. Specially, using the datasets in the calibration period 1915-1984, the variability of SCESR and SST were decomposed into interannual and interdecadal components. On the interannual timescale, a threshold PLS regression model was fitted to interannual components of SCESR and March SST patterns by taking account of the modulation of negative and positive phases of the Pacific Decadal Oscillation (PDO). On the interdecadal timescale, a standard PLS regression model was fitted to the relationship between SCESR and preceding November SST patterns. The total rainfall prediction was obtained by the sum of the outputs from both interannual and interdecadal models. Results show that the TSDTR downscaling approach achieved a reasonable skill to predict the observed rainfall in the validation period 1985-2006, compared to other simpler approaches. This study suggests that the TSDTR approach considering different interannual SCESR-SST relationships under the modulation of PDO phases, as well as the interdecadal variability of SCESR associated with SST patterns may provide a new perspective to improve the climate predictions.

  10. Effects of changes in vegetation on precipitation in the northern Tianshan Mountains evaluated using multiple time scales

    Indian Academy of Sciences (India)

    Qinming Qinming Sun; Tong Liu; Zhiquan Han; Yongping Wu; Bai-Lian Li

    2016-04-01

    This study used a combination of the wavelet cross-correlation technique and numerical analysis ofvegetative feedback to study the role of climate–vegetation feedback from 1981 to 2009 in the northernTianshan Mountains, Xinjiang Province, China. The study area included the Irtysh River, the Bortalaand Ili River valleys, the northern slopes of the Tianshan Mountains, and the western Junggar Basin.The feedback effects of changes in vegetation on precipitation appeared to vary in these five regionswhen different time scales are used to examine them. The most useful time scale was generally found tobe 4–6 months. Time lag was another characteristic of this process, and the optimal time lag was 3–4months. Nevertheless, optimal time scale and time lag did not differ significantly in these five regions. Inthis way, the correct time scale of the effects of variations in vegetation on precipitation in this cold, aridarea was found. This time scale and time lag can be assessed through wavelet cross-correlation analysis.Then numerical analysis can be used to improve the accuracy of the analysis.

  11. Climatic changes on orbital and sub-orbital time scale recorded by the Guliya ice core in Tibetan Plateau

    Institute of Scientific and Technical Information of China (English)

    姚檀栋; 徐柏青; 蒲健辰

    2001-01-01

    Based on ice core records in the Tibetan Plateau and Greenland, the features and possible causes of climatic changes on orbital and sub-orbital time scale were discussed. Orbital time scale climatic change recorded in ice core from the Tibetan Plateau is typically ahead of that from polar regions, which indicates that climatic change in the Tibetan Plateau might be earlier than polar regions. The solar radiation change is a major factor that dominates the climatic change on orbital time scale. However, climatic events on sub-orbital time scale occurred later in the Tibetan Plateau than in the Arctic Region, indicating a different mechanism. For example, the Younger Dryas and Heinrich events took place earlier in Greenland ice core record than in Guliya ice core record. It is reasonable to propose the hypothesis that these climatic events were affected possibly by the Laurentide Ice Sheet. Therefore, ice sheet is critically important to climatic change on sub-orbital time scale in some ice ages.

  12. Influence of baryonic physics on the merger time-scale of galaxies in N-body/hydrodynamical simulations

    CERN Document Server

    Jiang, C Y; Lin, W P

    2009-01-01

    Following our previous work(Jiang et al.(2008)), in which we studied the merger time-scale of galaxies in a high-resolution cosmological hydro/N-body simulation, we investigate the potential influence of uncertainties in the numerical implementation of baryonic physics on the merger time-scale. The simulation used in the previous work suffers from the overcooling problem which causes the central galaxies of large halos too massive. This may result in a shortened merger time-scale compared to that in the real universe. We run a similar simulation, but the stellar mass is significantly reduced to model another extreme case of low stellar mass. Our result shows that the merger time-scale is little affected by the star formation recipes, except for the satellites in nearly radial orbits which show a 22 percent higher time-scale in the lower stellar mass case. Since the radial orbits only account for a small part of the satellites' orbits, the fitting formula in Jiang et al.(2008) is still applicable to a reasonab...

  13. Analysis of illegal peptide biopharmaceuticals frequently encountered by controlling agencies.

    Science.gov (United States)

    Vanhee, Celine; Janvier, Steven; Desmedt, Bart; Moens, Goedele; Deconinck, Eric; De Beer, Jacques O; Courselle, Patricia

    2015-09-01

    Recent advances in genomics, recombinant expression technologies and peptide synthesis have led to an increased development of protein and peptide therapeutics. Unfortunately this goes hand in hand with a growing market of counterfeit and illegal biopharmaceuticals, including substances that are still under pre-clinical and clinical development. These counterfeit and illegal protein and peptide substances could imply severe health threats as has been demonstrated by numerous case reports. The Belgian Federal Agency for Medicines and Health Products (FAMHP) and customs are striving, together with their global counterparts, to curtail the trafficking and distributions of these substances. At their request, suspected protein and peptide preparations are analysed in our Official Medicines Control Laboratory (OMCL). It stands to reason that a general screening method would be beneficiary in the battle against counterfeit and illegal peptide drugs. In this paper we present such general screening method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the identification of counterfeit and illegal injectable peptide preparations, extended with a subsequent quantification method using ultra-high performance liquid chromatography with diode array detection (UHPLC-DAD). The screening method, taking only 30 min, is able to selectively detect 25 different peptides and incorporates the proposed minimum of five identification points (IP) as has been recommended for sports drug testing applications. The group of peptides represent substances which have already been detected in illegal and counterfeit products seized by different European countries as well as some biopharmaceutical peptides which have not been confiscated yet by the controlling agencies, but are already being used according to the many internet users forums. Additionally, we also show that when applying the same LC gradient, it is also possible to quantify these peptides without the need for

  14. Analysis of illegal peptide biopharmaceuticals frequently encountered by controlling agencies.

    Science.gov (United States)

    Vanhee, Celine; Janvier, Steven; Desmedt, Bart; Moens, Goedele; Deconinck, Eric; De Beer, Jacques O; Courselle, Patricia

    2015-09-01

    Recent advances in genomics, recombinant expression technologies and peptide synthesis have led to an increased development of protein and peptide therapeutics. Unfortunately this goes hand in hand with a growing market of counterfeit and illegal biopharmaceuticals, including substances that are still under pre-clinical and clinical development. These counterfeit and illegal protein and peptide substances could imply severe health threats as has been demonstrated by numerous case reports. The Belgian Federal Agency for Medicines and Health Products (FAMHP) and customs are striving, together with their global counterparts, to curtail the trafficking and distributions of these substances. At their request, suspected protein and peptide preparations are analysed in our Official Medicines Control Laboratory (OMCL). It stands to reason that a general screening method would be beneficiary in the battle against counterfeit and illegal peptide drugs. In this paper we present such general screening method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the identification of counterfeit and illegal injectable peptide preparations, extended with a subsequent quantification method using ultra-high performance liquid chromatography with diode array detection (UHPLC-DAD). The screening method, taking only 30 min, is able to selectively detect 25 different peptides and incorporates the proposed minimum of five identification points (IP) as has been recommended for sports drug testing applications. The group of peptides represent substances which have already been detected in illegal and counterfeit products seized by different European countries as well as some biopharmaceutical peptides which have not been confiscated yet by the controlling agencies, but are already being used according to the many internet users forums. Additionally, we also show that when applying the same LC gradient, it is also possible to quantify these peptides without the need for

  15. A cocoa peptide protects Caenorhabditis elegans from oxidative stress and β-amyloid peptide toxicity.

    Directory of Open Access Journals (Sweden)

    Patricia Martorell

    Full Text Available BACKGROUND: Cocoa and cocoa-based products contain different compounds with beneficial properties for human health. Polyphenols are the most frequently studied, and display antioxidant properties. Moreover, protein content is a very interesting source of antioxidant bioactive peptides, which can be used therapeutically for the prevention of age-related diseases. METHODOLOGY/PRINCIPAL FINDINGS: A bioactive peptide, 13L (DNYDNSAGKWWVT, was obtained from a hydrolyzed cocoa by-product by chromatography. The in vitro inhibition of prolyl endopeptidase (PEP was used as screening method to select the suitable fraction for peptide identification. Functional analysis of 13L peptide was achieved using the transgenic Caenorhabditis elegans strain CL4176 expressing the human Aβ₁₋₄₂ peptide as a pre-clinical in vivo model for Alzheimer's disease. Among the peptides isolated, peptide 13L (1 µg/mL showed the highest antioxidant activity (P≤0.001 in the wild-type strain (N2. Furthermore, 13L produced a significant delay in body paralysis in strain CL4176, especially in the 24-47 h period after Aβ₁₋₄₂ peptide induction (P≤0.0001. This observation is in accordance with the reduction of Aβ deposits in CL4176 by western blot. Finally, transcriptomic analysis in wild-type nematodes treated with 13L revealed modulation of the proteosomal and synaptic functions as the main metabolic targets of the peptide. CONCLUSIONS/SIGNIFICANCE: These findings suggest that the cocoa 13L peptide has antioxidant activity and may reduce Aβ deposition in a C. elegans model of Alzheimer's disease; and therefore has a putative therapeutic potential for prevention of age-related diseases. Further studies in murine models and humans will be essential to analyze the effectiveness of the 13L peptide in higher animals.

  16. The Influence of Time Scale on the Quantitative Study of Soil and Water Conservation Effect of Grassland

    Institute of Scientific and Technical Information of China (English)

    Xiaoxia; WU; Zhujun; GU

    2015-01-01

    Quantitative analysis of time scale effects is conducive to further understanding of vegetation water and soil conservation mechanism.Based on the observation data of the grass covered and bare soil( control) experimental plots located in Hetian Town,Changting County of Fujian Province from 2007 to 2010,the characteristics of 4 parameters( precipitation,vegetation,RE and SE) were analyzed at precipitation event,month,season,and annual scales,and then the linear regression models were established to describe the relationships between RE( SE)and its influencing factors of precipitation and vegetation. RE( SE) means the ratio of runoff depth( soil loss) of grass covered plot to that of the control plot. Results show that these 4 parameters presented different magnitude and variation on different time scales. RE and SE were relatively stable either within or among different time scales due to their ratios reducing the influence of other factors. The coupling of precipitation and vegetation led to better water conservation effect at lower RE( 0. 7) REs at precipitation event scale as well as at annual scale( R2> 0. 78). For the soil conservation effect,precipitation or / and vegetation was / were the dominated influence factor( s) at precipitation event and annual scales,and the grass LAI could basically describe the positive conservation effect( SE 0. 55),while the maximum 30 min intensity( I30) could describe the negative conservation effect more accurately( SE >1,R2> 0. 79). More uncertainties( R2≈0. 4) exist in the models of both RE and SE at two moderate time scales( month and season). Consequently,factors influencing water and soil conservation effect of grass present different variation and coupling characteristics on different time scales,indicating the importance of time scale at the study on water and soil conservation.

  17. The purification of affinity-labelled active-site peptides

    International Nuclear Information System (INIS)

    The isolation of the labelled peptide from the protein digest, following the affinity labelling of the active sites of enzymes or antibodies, is described. Single-step affinity chromatography utilises the affinity of the native enzymes or antibody for the ligand used to label the same protein. The labelled peptide is the only one in the digest that displays affinity for the immobilised protein and can be released with eluants that dissociate the protein-ligand complex. (Auth.)

  18. Necessary and Sufficient Conditions for the Existence of Positive Solution for Singular Boundary Value Problems on Time Scales

    Directory of Open Access Journals (Sweden)

    Meiqiang Feng

    2009-01-01

    Full Text Available By constructing available upper and lower solutions and combining the Schauder's fixed point theorem with maximum principle, this paper establishes sufficient and necessary conditions to guarantee the existence of Cld[0,1]𝕋 as well as CldΔ[0,1]𝕋 positive solutions for a class of singular boundary value problems on time scales. The results significantly extend and improve many known results for both the continuous case and more general time scales. We illustrate our results by one example.

  19. Delta-Nabla Type Maximum Principles for Second-Order Dynamic Equations on Time Scales and Applications

    Directory of Open Access Journals (Sweden)

    Jiang Zhu

    2014-01-01

    Full Text Available Some delta-nabla type maximum principles for second-order dynamic equations on time scales are proved. By using these maximum principles, the uniqueness theorems of the solutions, the approximation theorems of the solutions, the existence theorem, and construction techniques of the lower and upper solutions for second-order linear and nonlinear initial value problems and boundary value problems on time scales are proved, the oscillation of second-order mixed delat-nabla differential equations is discussed and, some maximum principles for second order mixed forward and backward difference dynamic system are proved.

  20. A Novel Lactic Acid Bacteria Growth-stimulating Peptide from Broad Bean (Vicia faba .) Protein Hydrolysates

    OpenAIRE

    Ping Xiao; Yuan Liu; Rizwan-ur-Rehman; Ran Kang; Yanping Wang

    2015-01-01

    In this study, broad bean protein hydrolysates (BPH) produced by alcalase with strong-stimulating activity for lactic acid bacteria (LAB) was first time reported. In order to obtain the key peptide that have growth-stimulating activity for lactic acid bacteria (LAB), gel filtration chromatography and Reverse Phase High Performance Liquid Chromatography (RP-HPLC) were applied to isolate and purify the peptides from BPH. Finally, F4-2 elicited the highest activity for LAB, corresponding to amin...

  1. Time scales and mechanisms of estuarine variability, a synthesis from studies of San Francisco Bay

    Science.gov (United States)

    Cloern, J.E.; Nichols, F.H.

    1985-01-01

    This review of the preceding papers suggests that temporal variability in San Francisco Bay can be characterized by four time scales (hours, days-weeks, months, years) and associated with at least four mechanisms (variations in freshwater inflow, tides, wind, and exchange with coastal waters). The best understood component of temporal variability is the annual cycle, which is most obviously influenced by seasonal variations in freshwater inflow. The winter season of high river discharge is characterized by: large-scale redistribution of the salinity field (e.g. the upper estuary becomes a riverine system); enhanced density stratification and gravitational circulation with shortened residence times in the bay; decreased tissue concentrations of some contaminants (e.g. copper) in resident bivalves; increased estuarine inputs of river-borne materials such as dissolved inorganic nutrients (N, P, Si), suspended sediments, and humic materials; radical redistributions of pelagic organisms such as copepods and fish; low phutoplankton biomass and primary productivity in the upper estuary; and elimination of freshwater-intolerant species of macroalgae and benthic infauna from the upper estuary. Other mechanisms modulate this river-driven annual cycle: (1) wind speed is highly seasonal (strongest in summer) and causes seasonal variations in atmosphere-water column exchange of dissolved gases, resuspension, and the texture of surficial sediments; (2) seasonal variations in the coastal ocean (e.g. the spring-summer upwelling season) influence species composition of plankton and nutrient concentrations that are advected into the bay; and (3) the annual temperature cycle influences a few selected features (e.g. production and hatching of copepod resting eggs). Much of the interannual variability in San Francisco Bay is also correlated with freshwater inflow: wet years with persistently high river discharge are characterized by persistent winter-type conditions. Mechanisms of

  2. Human peptide transporters

    DEFF Research Database (Denmark)

    Nielsen, Carsten Uhd; Brodin, Birger; Jørgensen, Flemming Steen;

    2002-01-01

    Peptide transporters are epithelial solute carriers. Their functional role has been characterised in the small intestine and proximal tubules, where they are involved in absorption of dietary peptides and peptide reabsorption, respectively. Currently, two peptide transporters, PepT1 and PepT2...

  3. Artificial neural networks for the prediction of peptide drift time in ion mobility mass spectrometry

    Directory of Open Access Journals (Sweden)

    Plasencia Manolo

    2010-04-01

    Full Text Available Abstract Background There is an increasing usage of ion mobility-mass spectrometry (IMMS in proteomics. IMMS combines the features of ion mobility spectrometry (IMS and mass spectrometry (MS. It separates and detects peptide ions on a millisecond time-scale. IMS separates peptide ions based on drift time that is determined by the collision cross-section of each peptide ion in a given experiment condition. A peptide ion's collision cross-section is related to the ion size and shape resulted from the peptide amino acid sequence and their modifications. This inherent relation between the drift time of peptide ion and peptide sequence indicates that the drift time of peptide ions can be used to infer peptide sequence and therefore, for peptide identification. Results This paper describes an artificial neural networks (ANNs regression model for the prediction of peptide ion drift time in IMMS. Each peptide in this work was represented using three descriptors (i.e., molecular weight, sequence length and a two-dimensional sequence index. An ANN predictor consisting of four input nodes, three hidden nodes and one output node was constructed for peptide ion drift time prediction. For the model training and testing, a 10-fold cross-validation strategy was employed for three datasets each containing different charge states. Dataset one contains 212 singly-charged peptide ions, dataset two has 306 doubly-charged peptide ions, and dataset three has 77 triply-charged peptide ions. Our proposed method achieved 94.4%, 93.6% and 74.2% prediction accuracy for singly-, doubly- and triply-charged peptide ions, respectively. Conclusions An ANN-based method has been developed for predicting the drift time of peptide ions in IMMS. The results achieved here demonstrate the effectiveness and efficiency of the prediction model. This work can enhance the confidence of protein identification by combining with current database search approaches for protein identification.

  4. Development of SI-traceable C-peptide certified reference material NMIJ CRM 6901-a using isotope-dilution mass spectrometry-based amino acid analyses.

    Science.gov (United States)

    Kinumi, Tomoya; Goto, Mari; Eyama, Sakae; Kato, Megumi; Kasama, Takeshi; Takatsu, Akiko

    2012-07-01

    A certified reference material (CRM) is a higher-order calibration material used to enable a traceable analysis. This paper describes the development of a C-peptide CRM (NMIJ CRM 6901-a) by the National Metrology Institute of Japan using two independent methods for amino acid analysis based on isotope-dilution mass spectrometry. C-peptide is a 31-mer peptide that is utilized for the evaluation of β-cell function in the pancreas in clinical testing. This CRM is a lyophilized synthetic peptide having the human C-peptide sequence, and contains deamidated and pyroglutamylated forms of C-peptide. By adding water (1.00 ± 0.01) g into the vial containing the CRM, the C-peptide solution in 10 mM phosphate buffer saline (pH 6.6) is reconstituted. We assigned two certified values that represent the concentrations of total C-peptide (mixture of C-peptide, deamidated C-peptide, and pyroglutamylated C-peptide) and C-peptide. The certified concentration of total C-peptide was determined by two amino acid analyses using pre-column derivatization liquid chromatography-mass spectrometry and hydrophilic chromatography-mass spectrometry following acid hydrolysis. The certified concentration of C-peptide was determined by multiplying the concentration of total C-peptide by the ratio of the relative area of C-peptide to that of the total C-peptide measured by liquid chromatography. The certified value of C-peptide (80.7 ± 5.0) mg/L represents the concentration of the specific entity of C-peptide; on the other hand, the certified value of total C-peptide, (81.7 ± 5.1) mg/L can be used for analyses that does not differentiate deamidated and pyroglutamylated C-peptide from C-peptide itself, such as amino acid analyses and immunochemical assays.

  5. Assessments of Drought Impacts on Vegetation in China with the Optimal Time Scales of the Climatic Drought Index

    Directory of Open Access Journals (Sweden)

    Zheng Li

    2015-07-01

    Full Text Available Drought is expected to increase in frequency and severity due to global warming, and its impacts on vegetation are typically extensively evaluated with climatic drought indices, such as multi-scalar Standardized Precipitation Evapotranspiration Index (SPEI. We analyzed the covariation between the SPEIs of various time scales and the anomalies of the normalized difference vegetation index (NDVI, from which the vegetation type-related optimal time scales were retrieved. The results indicated that the optimal time scales of needle-leaved forest, broadleaf forest and shrubland were between 10 and 12 months, which were considerably longer than the grassland, meadow and cultivated vegetation ones (2 to 4 months. When the optimal vegetation type-related time scales were used, the SPEI could better reflect the vegetation’s responses to water conditions, with the correlation coefficients between SPEIs and NDVI anomalies increased by 5.88% to 28.4%. We investigated the spatio-temporal characteristics of drought and quantified the different responses of vegetation growth to drought during the growing season (April–October. The results revealed that the frequency of drought has increased in the 21st century with the drying trend occurring in most of China. These results are useful for ecological assessments and adapting management steps to mitigate the impact of drought on vegetation. They are helpful to employ water resources more efficiently and reduce potential damage to human health caused by water shortages.

  6. Assessments of Drought Impacts on Vegetation in China with the Optimal Time Scales of the Climatic Drought Index.

    Science.gov (United States)

    Li, Zheng; Zhou, Tao; Zhao, Xiang; Huang, Kaicheng; Gao, Shan; Wu, Hao; Luo, Hui

    2015-07-08

    Drought is expected to increase in frequency and severity due to global warming, and its impacts on vegetation are typically extensively evaluated with climatic drought indices, such as multi-scalar Standardized Precipitation Evapotranspiration Index (SPEI). We analyzed the covariation between the SPEIs of various time scales and the anomalies of the normalized difference vegetation index (NDVI), from which the vegetation type-related optimal time scales were retrieved. The results indicated that the optimal time scales of needle-leaved forest, broadleaf forest and shrubland were between 10 and 12 months, which were considerably longer than the grassland, meadow and cultivated vegetation ones (2 to 4 months). When the optimal vegetation type-related time scales were used, the SPEI could better reflect the vegetation's responses to water conditions, with the correlation coefficients between SPEIs and NDVI anomalies increased by 5.88% to 28.4%. We investigated the spatio-temporal characteristics of drought and quantified the different responses of vegetation growth to drought during the growing season (April-October). The results revealed that the frequency of drought has increased in the 21st century with the drying trend occurring in most of China. These results are useful for ecological assessments and adapting management steps to mitigate the impact of drought on vegetation. They are helpful to employ water resources more efficiently and reduce potential damage to human health caused by water shortages.

  7. Time scale of entropic segregation of flexible polymers in confinement: Implications for chromosome segregation in filamentous bacteria

    OpenAIRE

    Arnold, Axel; Jun, Suckjoon

    2007-01-01

    We report molecular dynamics simulations of the segregation of two overlapping chains in cylindrical confinement. We find that the entropic repulsion between the chains can be sufficiently strong to cause segregation on a time scale that is short compared to the one for diffusion. This result implies that entropic driving forces are sufficiently strong to cause rapid bacterial chromosome segregation.

  8. An LMI Approach to Stability for Linear Time-Varying System with Nonlinear Perturbation on Time Scales

    OpenAIRE

    Kanit Mukdasai; Piyapong Niamsup

    2011-01-01

    We consider Lyapunov stability theory of linear time-varying system and derive sufficient conditions for uniform stability, uniform exponential stability, -uniform stability, and h-stability for linear time-varying system with nonlinear perturbation on time scales. We construct appropriate Lyapunov functions and derive several stability conditions. Numerical examples are presented to illustrate the effectiveness of the theoretical results.

  9. An LMI Approach to Stability for Linear Time-Varying System with Nonlinear Perturbation on Time Scales

    OpenAIRE

    Mukdasai, Kanit; Niamsup, Piyapong

    2011-01-01

    We consider Lyapunov stability theory of linear time-varying system and derive sufficient conditions for uniform stability, uniform exponential stability, -uniform stability, and h-stability for linear time-varying system with nonlinear perturbation on time scales. We construct appropriate Lyapunov functions and derive several stability conditions. Numerical examples are presented to illustrate the effectiveness of the theoretical results.

  10. Patterns of similarity of seasonal water balances: A window into streamflow variability over a range of time scales

    NARCIS (Netherlands)

    Berghuijs, W.R.; Sivapalan, M.; Woods, R.A.; Savenije, H.H.G.

    2014-01-01

    Recent hydrologic synthesis efforts have presented evidence that the seasonal water balance is at the core of overall catchment responses, and understanding it will assist in predicting signatures of streamflow variability at other time scales, including interannual variability, the flow duration cu

  11. Native American Students' Understanding of Geologic Time Scale: 4th-8th Grade Ojibwe Students' Understanding of Earth's Geologic History

    Science.gov (United States)

    Nam, Younkyeong; Karahan, Engin; Roehrig, Gillian

    2016-01-01

    Geologic time scale is a very important concept for understanding long-term earth system events such as climate change. This study examines forty-three 4th-8th grade Native American--particularly Ojibwe tribe--students' understanding of relative ordering and absolute time of Earth's significant geological and biological events. This study also…

  12. A novel way to detect correlations on multi-time scales, with temporal evolution and for multi-variables

    Science.gov (United States)

    Yuan, Naiming; Xoplaki, Elena; Zhu, Congwen; Luterbacher, Juerg

    2016-06-01

    In this paper, two new methods, Temporal evolution of Detrended Cross-Correlation Analysis (TDCCA) and Temporal evolution of Detrended Partial-Cross-Correlation Analysis (TDPCCA), are proposed by generalizing DCCA and DPCCA. Applying TDCCA/TDPCCA, it is possible to study correlations on multi-time scales and over different periods. To illustrate their properties, we used two climatological examples: i) Global Sea Level (GSL) versus North Atlantic Oscillation (NAO); and ii) Summer Rainfall over Yangtze River (SRYR) versus previous winter Pacific Decadal Oscillation (PDO). We find significant correlations between GSL and NAO on time scales of 60 to 140 years, but the correlations are non-significant between 1865–1875. As for SRYR and PDO, significant correlations are found on time scales of 30 to 35 years, but the correlations are more pronounced during the recent 30 years. By combining TDCCA/TDPCCA and DCCA/DPCCA, we proposed a new correlation-detection system, which compared to traditional methods, can objectively show how two time series are related (on which time scale, during which time period). These are important not only for diagnosis of complex system, but also for better designs of prediction models. Therefore, the new methods offer new opportunities for applications in natural sciences, such as ecology, economy, sociology and other research fields.

  13. Existence of Positive Solutions for Second-Order m-Point Boundary Value Problems on Time Scales

    Institute of Scientific and Technical Information of China (English)

    Pei-guang Wang; Ying Wang

    2006-01-01

    This paper investigates the existence of positive solutions of the m-point boundary value problem for second-order dynamic equations on time scales, and obtain the result that the problem has at least one positive solution by using functional-type cone expansion-compression fixed point theorem.

  14. Climate Change and Its Effects on Runoff of Kaidu River,Xinjiang, China: A Multiple Time-scale Analysis

    Institute of Scientific and Technical Information of China (English)

    XU Jianhua; CHEN Yaning; JI Minhe; LU Feng

    2008-01-01

    This paper applied an integrated method combining grey relation analysis, wavelet analysis and statistical analysis to study climate change and its effects on runoff of the Kaidu River at multi-time scales. Major findings are as follows: 1) Climatic factors were ranked in the order of importance to annual runoff as average annual temperature, average temperature in autumn, average temperature in winter, annual precipitation, precipitation in flood season, av-erage temperature in summer, and average temperature in spring. The average annual temperature and annual precipi-tation were selected as the two representative factors that impact the annual runoff. 2) From the 32-year time scale, the annual runoff and the average annual temperature presented a significantly rising trend, whereas the annual precipita-tion showed little increase over the period of 1957-2002. By changing the time scale from 32-year to 4-year, we ob-served nonlinear trends with increasingly obvious oscillations for annual runoff, average annual temperature, and an-nual precipitation. 3) The changes of the runoff and the regional climate are closely related, indicating that the runoff change is the result of the regional climate changes. With time scales ranging from 32-year, 16-year, 8-year and to 4-year, there are highly significant linear correlations between the annual runoff and the average annual temperature and the annual precipitation.

  15. Screening for N-glycosylated proteins by liquid chromatography mass spectrometry

    DEFF Research Database (Denmark)

    Bunkenborg, Jakob; Pilch, Bartosz J; Podtelejnikov, Alexandre V;

    2004-01-01

    in complex mixtures by reducing sample complexity and enriching glycoprotein content. Glycosylated proteins are selected by an initial lectin chromatography step and digested with endoproteinase Lys-C. Glycosylated peptides are then selected from the digest mixture by a second lectin chromatography step....... The glycan components are removed with N-glycosidase F and the peptides digested with trypsin before analysis by on-line reversed-phase liquid chromatography mass spectrometry. Using two different lectins, concanavalin A and wheat germ agglutinin, this procedure was applied to human serum and a total of 86 N...

  16. Taking care of business in a flash: constraining the time-scale for low-mass satellite quenching with ELVIS

    Science.gov (United States)

    Fillingham, Sean P.; Cooper, Michael C.; Wheeler, Coral; Garrison-Kimmel, Shea; Boylan-Kolchin, Michael; Bullock, James S.

    2015-12-01

    The vast majority of dwarf satellites orbiting the Milky Way and M31 are quenched, while comparable galaxies in the field are gas rich and star forming. Assuming that this dichotomy is driven by environmental quenching, we use the Exploring the Local Volume in Simulations (ELVIS) suite of N-body simulations to constrain the characteristic time-scale upon which satellites must quench following infall into the virial volumes of their hosts. The high satellite quenched fraction observed in the Local Group demands an extremely short quenching time-scale (˜2 Gyr) for dwarf satellites in the mass range M⋆ ˜ 106-108 M⊙. This quenching time-scale is significantly shorter than that required to explain the quenched fraction of more massive satellites (˜8 Gyr), both in the Local Group and in more massive host haloes, suggesting a dramatic change in the dominant satellite quenching mechanism at M⋆ ≲ 108 M⊙. Combining our work with the results of complementary analyses in the literature, we conclude that the suppression of star formation in massive satellites (M⋆ ˜ 108-1011 M⊙) is broadly consistent with being driven by starvation, such that the satellite quenching time-scale corresponds to the cold gas depletion time. Below a critical stellar mass scale of ˜108 M⊙, however, the required quenching times are much shorter than the expected cold gas depletion times. Instead, quenching must act on a time-scale comparable to the dynamical time of the host halo. We posit that ram-pressure stripping can naturally explain this behaviour, with the critical mass (of M⋆ ˜ 108 M⊙) corresponding to haloes with gravitational restoring forces that are too weak to overcome the drag force encountered when moving through an extended, hot circumgalactic medium.

  17. Biosphere-atmosphere exchange of CO2 in relation to climate: a cross-biome analysis across multiple time scales

    Directory of Open Access Journals (Sweden)

    L. Montagnani

    2009-04-01

    Full Text Available The biosphere-atmosphere flux of CO2 responds to climatic variability at time scales from seconds to years and longer. Quantifying the strength of the interaction between the flux and climate variables at multiple frequencies is necessary to begin understanding the climatic controls on the dynamics of the terrestrial carbon cycle. Orthonormal wavelet transformation (OWT can quantify the interaction between flux and microclimate at multiple frequencies while expressing time series variance in few energetic wavelet coefficients, offering a low-dimensional view of the measured climate-flux interaction. The variability of the net ecosystem exchange of CO2 (NEE, gross ecosystem productivity (GEP and ecosystem respiration (RE, and their co-variability with dominant climatic drivers, are explored with a global dataset consisting of 253 eddy covariance research sites. Results demonstrate that the NEE and GEP wavelet spectra are similar amongst plant functional types (PFT at weekly and shorter time scales, but significant divergence appeared among PFT at the biweekly and longer time scales, at which NEE and GEP are relatively less variable than climate. The RE spectra rarely differ among PFT across time scales. On average, RE spectra had greater low frequency (monthly to interannual variability than NEE, GEP and climate. The low frequency Fourier coefficients of eight sites with more than eight years of data were compared against CANOAK ecosystem model simulations. Both measurements and theory demonstrate that "multi-annual" spectral peaks in flux may emerge at low (4+ years time scales. Biological responses to climate and other internal system dynamics provide the likely explanation for observed multi-annual variability, but data records must be lengthened and measurements of ecosystem state must be made, and made available, to disentangle the mechanisms responsible for these patterns.

  18. Offline High pH Reversed-Phase Peptide Fractionation for Deep Phosphoproteome Coverage

    DEFF Research Database (Denmark)

    Batth, Tanveer S; Olsen, Jesper V

    2016-01-01

    peptide mixtures based on high pH (basic) reversed-phase chromatography prior to phosphopeptide enrichment and mass spectrometric analysis. Peptides are separated on a C18 reversed-phase column under basic conditions and fractions collected in timed intervals followed by concatenation of the fractions...

  19. Hydrolysis of Whey Protein Isolate with Bacillus licheniformis Protease: Fractionation and Identification of Aggregating Peptides

    NARCIS (Netherlands)

    Creusot, N.P.; Gruppen, H.

    2007-01-01

    The objective of this work was to identify the dominant aggregating peptides from a whey protein hydrolysate (degree of hydrolysis of 6.8%) obtained with Bacillus licheniformis protease. The aggregating peptides were fractionated with preparative reversed-phase chromatography and identified with liq

  20. Automated 2D peptide separation on a 1D nano-LC-MS system

    DEFF Research Database (Denmark)

    Taylor, Paul; Nielsen, Peter A; Trelle, Morten Beck;

    2009-01-01

    the on-line separation of highly complex peptide mixtures directly coupled with mass spectrometry-based identification. Here, we present a variation of the traditional MudPIT protocol, combining highly sensitive chromatography using a nanoflow liquid chromatography system (nano-LC) with a two...

  1. Factors Affecting the Inter-annual to Centennial Time Scale Variability of All Indian Summer Monsoon Rainfall

    Science.gov (United States)

    Malik, Abdul; Brönnimann, Stefan

    2016-04-01

    The All Indian Summer Monsoon Rainfall (AISMR) is highly important for the livelihood of more than 1 billion people living in the Indian sub-continent. The agriculture of this region is heavily dependent on seasonal (JJAS) monsoon rainfall. An early start or a slight delay of monsoon, or an early withdrawal or prolonged monsoon season may upset the farmer's agricultural plans, can cause significant reduction in crop yield, and hence economic loss. Understanding of AISMR is also vital because it is a part of global atmospheric circulation system. Several studies show that AISMR is influenced by internal climate forcings (ICFs) viz. ENSO, AMO, PDO etc. as well as external climate forcings (ECFs) viz. Greenhouse Gases, volcanic eruptions, and Total Solar Irradiance (TSI). We investigate the influence of ICFs and ECFs on AISMR using recently developed statistical technique called De-trended Partial-Cross-Correlation Analysis (DPCCA). DPCCA can analyse a complex system of several interlinked variables. Often, climatic variables, being cross correlated, are simultaneously tele-connected with several other variables and it is not easy to isolate their intrinsic relationship. In the presence of non-stationarities and background signals the calculated correlation coefficients can be overestimated and erroneous. DPCCA method removes the non-stationarities and partials out the influence of background signals from the variables being cross correlated and thus give a robust estimate of correlation. We have performed the analysis using NOAA Reconstructed SSTs and homogenised instrumental AISMR data set from 1854-1999. By employing the DPCCA method we find that there is a statistically insignificant negative intrinsic relation (by excluding the influence of ICFs, and ECFs except TSI) between AISMR and TSI on decadal to centennial time scale. The ICFs considerably modulate the relation between AISMR and solar activity between 50-80 year time scales and transform this relationship

  2. SIMAC - A phosphoproteomic strategy for the rapid separation of mono-phosphorylated from multiply phosphorylated peptides

    DEFF Research Database (Denmark)

    Thingholm, Tine E; Jensen, Ole N; Robinson, Phillip J;

    2008-01-01

    spectrometric analysis, such as immobilized metal affinity chromatography or titanium dioxide the coverage of the phosphoproteome of a given sample is limited. Here we report a simple and rapid strategy - SIMAC - for sequential separation of mono-phosphorylated peptides and multiply phosphorylated peptides from...... and an optimized titanium dioxide chromatographic method. More than double the total number of identified phosphorylation sites was obtained with SIMAC, primarily from a three-fold increase in recovery of multiply phosphorylated peptides....

  3. Modelling the water balance of a precise weighable lysimeter for short time scales

    Science.gov (United States)

    Fank, Johann; Klammler, Gernot; Rock, Gerhard

    2015-04-01

    Precise knowledge of the water fluxes between the atmosphere and the soil-plant system and the percolation to the groundwater system is of great importance for understanding and modeling water, solute and energy transfer in the atmosphere-plant-soil-groundwater system. Weighable lysimeters yield the most precise and realistic measures for the change of stored water volume (ΔS), Precipitation (P) which can be rain, irrigation, snow and dewfall and evapotranspiration (ET) as the sum of soil evaporation, evaporation of intercepted water and transpiration. They avoid systematic errors of standard gauges and class-A pans. Lysimeters with controlled suction at the lower boundary allow estimation of capillary rise (C) and leachate (L) on short time scales. Precise weighable large scale (surface >= 1 m2) monolithic lysimeters avoiding oasis effects allow to solve the water balance equation (P - ET - L + C ± ΔS = 0) for a 3D-section of a natural atmosphere-plant-soil-system for a certain time period. Precision and accuracy of the lysimeter measurements depend not only on the precision of the weighing device but also on external conditions, which cannot be controlled or turned off. To separate the noise in measured data sets from signals the adaptive window and adaptive threshold (AWAT) filter (Peters et al., 2014) is used. The data set for the years 2010 and 2011 from the HYDRO-lysimeter (surface = 1 m2, depth = 1 m) in Wagna, Austria (Klammler and Fank, 2014) with a resolution of 0,01 mm for the lysimeter scale and of 0,001 mm for the leachate tank scale is used to evaluate the water balance. The mass of the lysimeter and the mass of the leachate tank is measured every two seconds. The measurements are stored as one minute arithmetic means. Based on calculations in a calibration period from January to May 2010 with different widths of moving window the wmax - Parameter for the AWAT filter was set to 41 minutes. A time series for the system mass ('upper boundary') of the

  4. Modelling the water balance of a precise weighable lysimeter for short time scales

    Science.gov (United States)

    Fank, Johann; Klammler, Gernot; Rock, Gerhard

    2015-04-01

    Precise knowledge of the water fluxes between the atmosphere and the soil-plant system and the percolation to the groundwater system is of great importance for understanding and modeling water, solute and energy transfer in the atmosphere-plant-soil-groundwater system. Weighable lysimeters yield the most precise and realistic measures for the change of stored water volume (ΔS), Precipitation (P) which can be rain, irrigation, snow and dewfall and evapotranspiration (ET) as the sum of soil evaporation, evaporation of intercepted water and transpiration. They avoid systematic errors of standard gauges and class-A pans. Lysimeters with controlled suction at the lower boundary allow estimation of capillary rise (C) and leachate (L) on short time scales. Precise weighable large scale (surface >= 1 m2) monolithic lysimeters avoiding oasis effects allow to solve the water balance equation (P - ET - L + C ± ΔS = 0) for a 3D-section of a natural atmosphere-plant-soil-system for a certain time period. Precision and accuracy of the lysimeter measurements depend not only on the precision of the weighing device but also on external conditions, which cannot be controlled or turned off. To separate the noise in measured data sets from signals the adaptive window and adaptive threshold (AWAT) filter (Peters et al., 2014) is used. The data set for the years 2010 and 2011 from the HYDRO-lysimeter (surface = 1 m2, depth = 1 m) in Wagna, Austria (Klammler and Fank, 2014) with a resolution of 0,01 mm for the lysimeter scale and of 0,001 mm for the leachate tank scale is used to evaluate the water balance. The mass of the lysimeter and the mass of the leachate tank is measured every two seconds. The measurements are stored as one minute arithmetic means. Based on calculations in a calibration period from January to May 2010 with different widths of moving window the wmax - Parameter for the AWAT filter was set to 41 minutes. A time series for the system mass ('upper boundary') of the

  5. Anion-exchange chromatography of phosphopeptides: weak anion exchange versus strong anion exchange and anion-exchange chromatography versus electrostatic repulsion-hydrophilic interaction chromatography.

    Science.gov (United States)

    Alpert, Andrew J; Hudecz, Otto; Mechtler, Karl

    2015-01-01

    Most phosphoproteomics experiments rely on prefractionation of tryptic digests before online liquid chromatography-mass spectrometry. This study compares the potential and limitations of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) and anion-exchange chromatography (AEX). At a pH higher than 5, phosphopeptides have two negative charges per residue and are well-retained in AEX. However, peptides with one or two phosphate groups are not separated from peptides with multiple Asp or Glu residues, interfering with the identification of phosphopeptides. At a pH of 2, phosphate residues have just a single negative charge but Asp and Glu are uncharged. This facilitates the separation of phosphopeptides from unmodified acidic peptides. Singly phosphorylated peptides are retained weakly under these conditions, due to electrostatic repulsion, unless hydrophilic interaction is superimposed in the ERLIC mode. Weak anion-exchange (WAX) and strong anion-exchange (SAX) columns were compared, with both peptide standards and a HeLa cell tryptic digest. The SAX column exhibited greater retention at pH 6 than did the WAX column. However, only about 60% as many phosphopeptides were identified with SAX at pH 6 than via ERLIC at pH 2. In one ERLIC run, 12 467 phosphopeptides were identified, including 4233 with more than one phosphate. We conclude that chromatography of phosphopeptides is best performed at low pH in the ERLIC mode. Under those conditions, the performances of the SAX and WAX materials were comparable. The data have been deposited with the ProteomeXchange with identifier PXD001333. PMID:25827581

  6. Peptide Bond Isomerization in High-Temperature Simulations.

    Science.gov (United States)

    Neale, Chris; Pomès, Régis; García, Angel E

    2016-04-12

    Force fields for molecular simulation are generally optimized to model macromolecules such as proteins at ambient temperature and pressure. Nevertheless, elevated temperatures are frequently used to enhance conformational sampling, either during system setup or as a component of an advanced sampling technique such as temperature replica exchange. Because macromolecular force fields are now put upon to simulate temperatures and time scales that greatly exceed their original design specifications, it is appropriate to re-evaluate whether these force fields are up to the task. Here, we quantify the rates of peptide bond isomerization in high-temperature simulations of three octameric peptides and a small fast-folding protein. We show that peptide octamers with and without proline residues undergo cis/trans isomerization every 1-5 ns at 800 K with three classical atomistic force fields (AMBER99SB-ILDN, CHARMM22/CMAP, and OPLS-AA/L). On the low microsecond time scale, these force fields permit isomerization of nonprolyl peptide bonds at temperatures ≥500 K, and the CHARMM22/CMAP force field permits isomerization of prolyl peptide bonds ≥400 K. Moreover, the OPLS-AA/L force field allows chiral inversion about the Cα atom at 800 K. Finally, we show that temperature replica exchange permits cis peptide bonds developed at 540 K to subsequently migrate back to the 300 K ensemble, where cis peptide bonds are present in 2 ± 1% of the population of Trp-cage TC5b, including up to 4% of its folded state. Further work is required to assess the accuracy of cis/trans isomerization in the current generation of protein force fields. PMID:26866899

  7. Purification of a Novel Antibacterial Short Peptide in Earthworm Eisenia foetida

    Institute of Scientific and Technical Information of China (English)

    Yan-Qin LIU; Zhen-Jun SUN; Chong WANG; Shi-Jie LI; Yu-Zhi LIU

    2004-01-01

    A novel antimicrobial short peptide was purified from earthworm (Eisenia foetida) by a five-step protocol including ammonium sulfate precipitation, ultrafiltration, DE-52 ion exchange chromatography, Sephadex G-10 column chromatography, and C-18 reversed-phase HPLC techniques.The purified peptide was applied to the MALDI-TOP MS to determine the molecular mass and was also subjected to TOF MS-MS analysis to determine the amino acid sequence. As a result, a novel antibacterial peptide, named OEP3121, was obtained, with the molecular mass of 510.8 Da and the sequence being "ACSAG".

  8. Physicochemical properties determining the detection probability of tryptic peptides in Fourier transform mass spectrometry. A correlation study

    DEFF Research Database (Denmark)

    Nielsen, Michael L; Savitski, Mikhail M; Kjeldsen, Frank;

    2004-01-01

    Sequence verification and mapping of posttranslational modifications require nearly 100% sequence coverage in the "bottom-up" protein analysis. Even in favorable cases, routine liquid chromatography-mass spectrometry detects from protein digests peptides covering 50-90% of the sequence. Here we...... investigated the reasons for limited peptide detection, considering various physicochemical aspects of peptide behavior in liquid chromatography-Fourier transform mass spectrometry (LC-FTMS). No overall correlation was found between the detection probability and peptide mass. In agreement with literature data......, the signal increased with peptide hydrophobicity. Surprisingly, the pI values exhibited an opposite trend, with more acidic tryptic peptides detected with higher probability. A mixture of synthesized peptides of similar masses confirmed the hydrophobicity dependence but showed strong positive correlation...

  9. Eddy-correlation measurements of benthic fluxes under complex flow conditions: Effects of coordinate transformations and averaging time scales

    DEFF Research Database (Denmark)

    Lorke, Andreas; McGinnis, Daniel F.; Maeck, Andreas

    2013-01-01

    hours of continuous eddy-correlation measurements of sediment oxygen fluxes in an impounded river, we demonstrate that rotation of measured current velocities into streamline coordinates can be a crucial and necessary step in data processing under complex flow conditions in non-flat environments...... with complex topography. We found that under these conditions neither time series detrending nor coordinate rotation can remove low-frequency velocity variations completely. These variations result in spurious flux contributions and in a pronounced dependence of the derived fluxes on averaging time scales......Eddy-correlation measurements of sediment oxygen uptake rates in aquatic systems are increasingly used to obtain areal-averaged fluxes with a high temporal resolution. Here we discuss the effects of coordinate rotation and averaging time scale for Reynolds decomposition on flux estimates. Using 119...

  10. Computing the transport time scales of a stratified lake on the basis of Tonolli’s model

    Directory of Open Access Journals (Sweden)

    Marco Pilotti

    2014-05-01

    Full Text Available This paper deals with a simple model to evaluate the transport time scales in thermally stratified lakes that do not necessarily completely mix on a regular annual basis. The model is based on the formalization of an idea originally proposed in Italian by Tonolli in 1964, who presented a mass balance of the water initially stored within a lake, taking into account the known seasonal evolution of its thermal structure. The numerical solution of this mass balance provides an approximation to the water age distribution for the conceptualised lake, from which an upper bound to the typical time scales widely used in limnology can be obtained. After discussing the original test case considered by Tonolli, we apply the model to Lake Iseo, a deep lake located in the North of Italy, presenting the results obtained on the basis of a 30 year series of data.

  11. Microscopic description of quantum Lorentz gas and extension of the Boltzmann equation to entire space-time scale

    Science.gov (United States)

    Hashimoto, K.; Kanki, K.; Tanaka, S.; Petrosky, T.

    2016-02-01

    Irreversible processes of weakly coupled one-dimensional quantum perfect Lorentz gas are studied on the basis of the fundamental laws of physics in terms of the complex spectral analysis associated with the resonance state of the Liouville-von Neumann operator. Without any phenomenological operations, such as a coarse-graining of space-time, or a truncation of the higher order correlation, we obtained irreversible processes in a purely dynamical basis in all space and time scale including the microscopic atomic interaction range that is much smaller than the mean-free length. Based on this solution, a limitation of the usual phenomenological Boltzmann equation, as well as an extension of the Boltzmann equation to entire space-time scale, is discussed.

  12. Interval Oscillation Criteria for Forced Second-Order Nonlinear Delay Dynamic Equations with Damping and Oscillatory Potential on Time Scales

    Directory of Open Access Journals (Sweden)

    Hassan A. Agwa

    2016-01-01

    Full Text Available We are concerned with the interval oscillation of general type of forced second-order nonlinear dynamic equation with oscillatory potential of the form rtg1xt,xΔtΔ+p(tg2(x(t,xΔ(txΔ(t+q(tf(x(τ(t=e(t, on a time scale T. We will use a unified approach on time scales and employ the Riccati technique to establish some oscillation criteria for this type of equations. Our results are more general and extend the oscillation criteria of Erbe et al. (2010. Also our results unify the oscillation of the forced second-order nonlinear delay differential equation and the forced second-order nonlinear delay difference equation. Finally, we give some examples to illustrate our results.

  13. Reference evapotranspiration models using different time scales in the Jaboticabal region of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Natália Buzinaro Caporusso

    2014-11-01

    Full Text Available The aim of this paper is to compare 18 reference evapotranspiration models to the standard Penman-Monteith model in the Jaboticabal, São Paulo, region for the following time scales: daily, 5-day, 15-day and seasonal. A total of 5 years of daily meteorological data was used for the following analyses: accuracy (mean absolute percentage error, Mape, precision (R2 and tendency (bias (systematic error, SE. The results were also compared at the 95% probability level with Tukey’s test. The Priestley-Taylor (1972 method was the most accurate for all time scales, the Tanner-Pelton (1960 method was the most accurate in the winter, and the Thornthwaite (1948 method was the most accurate of the methods that only used temperature data in the equations.

  14. Chromatography resin support

    Science.gov (United States)

    Dobos, James G.

    2002-01-01

    An apparatus and method of using an improved chromatography resin support is disclosed. The chromatography support platform is provided by a stainless steel hollow cylinder adapted for being inserted into a chromatography column. An exterior wall of the stainless steel cylinder defines a groove for carrying therein an "O"-ring. The upper surface of the stainless steel column is covered by a fine stainless steel mesh welded to the edges of the stainless steel cylinder. When placed upon a receiving ledge defined within a chromatography column, the "O"-ring provides a fluid tight seal with the inner edge wall of the chromatography cylinder. The stainless steel mesh supports the chromatography matrix and provides a back flushable support which is economical and simple to construct.

  15. The Contribution of Wide Range of Space and Time Scales to the Northward Flux of Westerly Momentum

    Science.gov (United States)

    Straus, D. M.

    1982-01-01

    The contribution of a wide range of time scales to the long time average of atmospheric variances and covariances, and in particular on the contribution of the annual cycle is discussed. The interannual variability of seasonally averaged fluxes is analyzed in the same context. For definiteness, numerical results will be shown for the eddy momentum flux at 200 mb as obtained from seven years of NMC analyses (March 8, 1970 - March 10, 1977). The role of the seasonal cycle is considered.

  16. Systematic study of the effects of mass and time scaling techniques applied in numerical rock mechanics simulations

    Science.gov (United States)

    Heinze, Thomas; Jansen, Gunnar; Galvan, Boris; Miller, Stephen A.

    2016-08-01

    Numerical modeling is a well established tool in rock mechanics studies investigating a wide range of problems. Implicit methods for solving linear equations have the advantage of being unconditionally stable, while explicit methods, although limited by the time step, are often used because of their limited memory demand, their scalability in parallel computing, and simple implementation of complex boundary conditions. In numerical modeling of explicit elastoplastic dynamics where the time step is limited by the material density, mass scaling techniques can be used to overcome this limit and significantly reduce computation time. While often used, the effect of mass and time scaling and how it may influence the numerical results is rarely-mentioned in publications, and choosing the right scaling technique is typically performed by trial and error. To our knowledge, no systematic studies have addressed how mass scaling might affect the numerical results. In this paper, we present results from an extensive and systematic study of the influence of mass and time scaling on the behavior of a variety of rock-mechanical models. We employ a finite difference scheme to model uniaxial and biaxial compression experiments using different mass and time scaling factors, and with physical models of increasing complexity up to a cohesion-weakening frictional-strengthening model (CWFS). We also introduce a normalized energy ratio to assist analyzing mass scaling effects. We find the tested models to be less sensitive to time scaling than to mass scaling, so mass scaling has higher potential for decreasing computational costs. However, we also demonstrate that mass scaling may lead to quantitatively wrong results, so care must be taken in interpreting stress values when mass scaling is used in complicated rock mechanics simulations. Mass scaling significantly influences the stress-strain response of numerical rocks because mass scaling acts as an artificial hardening agent on rock

  17. Stabilization of large drainage basins over geological time scales : Cenozoic West Africa, hot spot swell growth, and the Niger River

    OpenAIRE

    Chardon, Dominique; Grimaud, J. L.; Rouby, D.; BEAUVAIS, Anicet; Christophoul, F.

    2016-01-01

    Reconstructing the evolving geometry of large river catchments over geological time scales is crucial to constraining yields to sedimentary basins. In the case of Africa, it should further help deciphering the response of large cratonic sediment routing systems to Cenozoic growth of the basin-and-swell topography of the continent. Mapping of dated and regionally correlated lateritic paleolandscape remnants complemented by onshore sedimentological archives allows the reconstruction...

  18. Power of the power-laws: lessons from unification of small and large time scales for evolution

    OpenAIRE

    Chowdhury, Debashish; Stauffer, Dietrich; Kunwar, Ambarish

    2002-01-01

    We develop a ``unified'' model that describes both ``micro'' and ``macro'' evolutions within a single theoretical framework. The eco-system is described as a dynamic network; the population dynamics at each node of this network describes the ``micro''-evolution over ecological time scales (i.e., birth, ageing and natural death of individual organisms) while the appearance of new nodes, the slow changes of the links and the disappearance of existing nodes accounts for the ``macro'' evolution o...

  19. A Study on Evalution of Local Time Scale%本地时标评价方法的研究

    Institute of Scientific and Technical Information of China (English)

    黄艳; 高源

    2012-01-01

    Apparently, whether local time scale runs good depends on how it is close to international standard time UTC, but it is more important that the consistency of the second definition reproduced by local time scale and by standard time UTC. Based on the concept defined by BIPM about International Atomic Time (TAI) and Coordinated Universal Time (UTC) , it is proposed that fractional frequency difference and time stability are the technical criteria to evaluate the local time scale. The argument above is proved by data analysis from six worldwide timekeeping laboratories with fractional frequency difference and time stability, and then this dissertation give some specific analysis about the relationship between each laboratory' s time scale generation program and the two indexes.%本地时标UTC(k)运行好坏取决于其与国际标准时间UTC的靠近程度,实际上,本地时标所复现的秒定义与UTC所复现的秒定义之间的一致程度应是更重要的衡量指标.根据国际计量局关于国际标准时间TAI和UTC的定义,提出了相对频率偏差(频率准确度)和时间稳定度是评价本地时标优劣的技术指标.通过对国内外6个守时实验室相对频率偏差、时间稳定度两项指标分析证明了上述观点,并对各实验室时标实现方案进行了简要评述.

  20. PeptideAtlas

    Data.gov (United States)

    U.S. Department of Health & Human Services — PeptideAtlas is a multi-organism, publicly accessible compendium of peptides identified in a large set of tandem mass spectrometry proteomics experiments. Mass...

  1. Multiple time scale evaluation of the energy balance during the maize growing season, and a new reason for energy imbalance

    Institute of Scientific and Technical Information of China (English)

    GUO JianXia; BIAN LinGen; DAI YongJiu

    2009-01-01

    Energy imbalance is a common problem associated with the measurement of surface energy using the eddy covariance method. In the evaluation of the energy balance, people usually pay more attention to the statistical result that the effective energy (the sum of sensible and latent heat) is systematically lower than the available energy (the difference of net radiation and ground heat flux). However, little attention has been paid to the existence of the reversed situation when the effective energy is larger than the available energy or their contribution to the overall energy closure rate. In this paper, based on the analysis of the energy balance on multiple time scales across the maize growth season, we con clude that the non-synchronization of energy components is the main reason for the existence of the reversed case. By shifting the phase of the effective energy components half an hour ahead, the rates of energy closure over all time scales are improved and dramatically reduce the number of the half-hourly samples when the energy ratio exceeds 1 or is below 0.5. According to the characteristics of the energy distribution and transformation over multiple time scales, latent heat is always the main type of energy cost, and the residual of the energy balance increases with the growth of the maize plant surpassing the sensible heat for seventy days. It is suggested that the heat storage and photosynthetic energy play an important role in the energy balance during the growing period of maize.

  2. Estimation of Dynamic VaR in Chinese Stock Markets Based on Time Scale and Extreme Value Theory

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The accuracy and time scale invariance of value-at-risk (VaR) measurement methods for different stock indices and at different confidence levels are tested. Extreme value theory (EVT) is applied to model the extreme tail of standardized residual series of daily/weekly indices losses, and parametric and nonparametric methods are used to estimate parameters of the general Pareto distribution (GPD), and dynamic VaR for indices of three stock markets in China. The accuracy and time scale invariance of risk measurement methods through back-testing approach are also examined. Results show that not all the indices accept time scale invariance; there are some differences in accuracy between different indices at various confidence levels. The most powerful dynamic VaR estimation methods are EVT-GJR-Hill at 97.5% level for weekly loss to Shanghai stock market, and EVT-GARCH-MLE (Hill) at 99.0% level for weekly loss to Taiwan and Hong Kong stock markets, respectively.

  3. PH dependent adhesive peptides

    Science.gov (United States)

    Tomich, John; Iwamoto, Takeo; Shen, Xinchun; Sun, Xiuzhi Susan

    2010-06-29

    A novel peptide adhesive motif is described that requires no receptor or cross-links to achieve maximal adhesive strength. Several peptides with different degrees of adhesive strength have been designed and synthesized using solid phase chemistries. All peptides contain a common hydrophobic core sequence flanked by positively or negatively charged amino acids sequences.

  4. Antimicrobial Peptides in 2014

    Directory of Open Access Journals (Sweden)

    Guangshun Wang

    2015-03-01

    Full Text Available This article highlights new members, novel mechanisms of action, new functions, and interesting applications of antimicrobial peptides reported in 2014. As of December 2014, over 100 new peptides were registered into the Antimicrobial Peptide Database, increasing the total number of entries to 2493. Unique antimicrobial peptides have been identified from marine bacteria, fungi, and plants. Environmental conditions clearly influence peptide activity or function. Human α-defensin HD-6 is only antimicrobial under reduced conditions. The pH-dependent oligomerization of human cathelicidin LL-37 is linked to double-stranded RNA delivery to endosomes, where the acidic pH triggers the dissociation of the peptide aggregate to release its cargo. Proline-rich peptides, previously known to bind to heat shock proteins, are shown to inhibit protein synthesis. A model antimicrobial peptide is demonstrated to have multiple hits on bacteria, including surface protein delocalization. While cell surface modification to decrease cationic peptide binding is a recognized resistance mechanism for pathogenic bacteria, it is also used as a survival strategy for commensal bacteria. The year 2014 also witnessed continued efforts in exploiting potential applications of antimicrobial peptides. We highlight 3D structure-based design of peptide antimicrobials and vaccines, surface coating, delivery systems, and microbial detection devices involving antimicrobial peptides. The 2014 results also support that combination therapy is preferred over monotherapy in treating biofilms.

  5. Peptide Nucleic Acid Synthons

    DEFF Research Database (Denmark)

    2004-01-01

    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  6. Peptide Nucleic Acids

    DEFF Research Database (Denmark)

    2003-01-01

    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  7. Peptide Nucleic Acids

    DEFF Research Database (Denmark)

    1998-01-01

    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  8. Peptide Nucleic Acids (PNA)

    DEFF Research Database (Denmark)

    2002-01-01

    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  9. Peptide-Carrier Conjugation

    DEFF Research Database (Denmark)

    Hansen, Paul Robert

    2015-01-01

    To produce antibodies against synthetic peptides it is necessary to couple them to a protein carrier. This chapter provides a nonspecialist overview of peptide-carrier conjugation. Furthermore, a protocol for coupling cysteine-containing peptides to bovine serum albumin is outlined....

  10. Periodic Solutions for Shunting Inhibitory Cellular Neural Networks of Neutral Type with Time-Varying Delays in the Leakage Term on Time Scales

    OpenAIRE

    Yongkun Li; Lei Wang; Yu Fei

    2014-01-01

    A class of shunting inhibitory cellular neural networks of neutral type with time-varying delays in the leakage term on time scales is proposed. Based on the exponential dichotomy of linear dynamic equations on time scales, fixed point theorems, and calculus on time scales we obtain some sufficient conditions for the existence and global exponential stability of periodic solutions for that class of neural networks. The results of this paper are completely new and complementary to the previous...

  11. Pseudo almost periodic solutions for neutral type high-order Hopfield neural networks with mixed time-varying delays and leakage delays on time scales

    OpenAIRE

    Li, Yongkun; Meng, Xiaofang; Xiong, Lianglin

    2015-01-01

    In this paper, a class of neutral type high-order Hopfield neural networks with mixed time-varying delays and leakage delays on time scales is proposed. Based on the exponential dichotomy of linear dynamic equations on time scales, Banach's fixed point theorem and the theory of calculus on time scales, some sufficient conditions are obtained for the existence and global exponential stability of pseudo almost periodic solutions for this class of neural networks. Our results are completely new....

  12. Periodic Solutions for Shunting Inhibitory Cellular Neural Networks of Neutral Type with Time-Varying Delays in the Leakage Term on Time Scales

    Directory of Open Access Journals (Sweden)

    Yongkun Li

    2014-01-01

    Full Text Available A class of shunting inhibitory cellular neural networks of neutral type with time-varying delays in the leakage term on time scales is proposed. Based on the exponential dichotomy of linear dynamic equations on time scales, fixed point theorems, and calculus on time scales we obtain some sufficient conditions for the existence and global exponential stability of periodic solutions for that class of neural networks. The results of this paper are completely new and complementary to the previously known results even if the time scale =ℝ or ℤ. Moreover, we present illustrative numerical examples to show the feasibility of our results.

  13. Novel antifungal peptides from Ceylon spinach seeds.

    Science.gov (United States)

    Wang, H; Ng, T B

    2001-11-01

    Two novel antifungal peptides, designated alpha- and beta-basrubrins, respectively, were isolated from seeds of the Ceylon spinach Basella rubra. The purification procedure involved saline extraction, (NH(4))(2)SO(4) precipitation, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose and FPLC-gel filtration on Superdex peptide column. alpha- and beta-basrubrins exhibited a molecular weight of 4.3 and 5 kDa, respectively. They inhibited translation in a rabbit reticulocyte system with an IC(50) value of 400 and 100 nM, respectively. alpha- and beta-basrubrin inhibited HIV-1 reverse transcriptase by (79.4 +/- 7.8)% and (54.6 +/- 3.6)%, respectively, at a concentration of 400 microM, and (10.56 +/- 0.92)% and (2.12 +/- 0.81)%, respectively, at a concentration of 40 microM. Both alpha- and beta-basrubrins exerted potent antifungal activity toward Botrytis cinerea, Mycosphaerella arachidicola, and Fusarium oxysporum. PMID:11688973

  14. Column Liquid Chromatography.

    Science.gov (United States)

    Majors, Ronald E.; And Others

    1984-01-01

    Reviews literature covering developments of column liquid chromatography during 1982-83. Areas considered include: books and reviews; general theory; columns; instrumentation; detectors; automation and data handling; multidimensional chromatographic and column switching techniques; liquid-solid chromatography; normal bonded-phase, reversed-phase,…

  15. Liquid Chromatography in 1982.

    Science.gov (United States)

    Freeman, David H.

    1982-01-01

    Reviews trends in liquid chromatography including apparatus, factors affecting efficient separation of a mixture (peak sharpness and speed), simplified problem-solving, adsorption, bonded phase chromatography, ion selectivity, and size exclusion. The current trend is to control chemical selectivity by the liquid phase. (Author/JN)

  16. Improved Methods for the Enrichment and Analysis of Glycated Peptides

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Qibin; Schepmoes, Athena A; Brock, Jonathan W; Wu, Si; Moore, Ronald J; Purvine, Samuel O; Baynes, John; Smith, Richard D; Metz, Thomas O

    2008-12-15

    Non-enzymatic glycation of tissue proteins has important implications in the development of complications of diabetes mellitus. Herein we report improved methods for the enrichment and analysis of glycated peptides using boronate affinity chromatography and electron transfer dissociation mass spectrometry, respectively. The enrichment of glycated peptides was improved by replacing an off-line desalting step with an on-line wash of column-bound glycated peptides using 50 mM ammonium acetate. The analysis of glycated peptides by MS/MS was improved by considering only higher charged (≥3) precursor-ions during data-dependent acquisition, which increased the number of glycated peptide identifications. Similarly, the use of supplemental collisional activation after electron transfer (ETcaD) resulted in more glycated peptide identifications when the MS survey scan was acquired with enhanced resolution. In general, acquiring ETD-MS/MS data at a normal MS survey scan rate, in conjunction with the rejection of both 1+ and 2+ precursor-ions, increased the number of identified glycated peptides relative to ETcaD or the enhanced MS survey scan rate. Finally, an evaluation of trypsin, Arg-C, and Lys-C showed that tryptic digestion of glycated proteins was comparable to digestion with Lys-C and that both were better than Arg-C in terms of the number glycated peptides identified by LC-MS/MS.

  17. Probing the hard and intermediate states of X-ray binaries using short time-scale variability

    Science.gov (United States)

    Skipper, Chris J.; McHardy, Ian M.

    2016-05-01

    Below an accretion rate of approximately a few per cent of the Eddington accretion rate, X-ray binary systems are not usually found in the soft spectral state. However, at accretion rates a factor of a few lower still, in the hard state, there is another spectral transition which is well observed but not well understood. Below {˜ }0.5-1 per cent of the Eddington accretion rate (dot{m}_crit), the spectral index hardens with increasing accretion rate, but above dot{m}_crit, although still in the hard state, the spectral index softens with increasing accretion rate. Here we use a combination of X-ray spectral fitting and a study of short time-scale spectral variability to examine the behaviour of three well-known X-ray binaries: Cygnus X-1, GX 339-4 and XTE J1118+480. In Cygnus X-1 we find separate hard and soft continuum components, and show using root mean square (rms) spectra that the soft component dominates the variability. The spectral transition at dot{m}_crit is clearly present in the hard-state hardness-intensity diagrams of Cygnus X-1. Above dot{m}_crit, GX 339-4 shows similar softer-when-brighter behaviour at both long and short time-scales. Similarly, XTE J1118+480, which remains well below dot{m}_crit, has harder-when-brighter behaviour on all time-scales. We interpret these results in terms of two continuum components: a hard power law which dominates the spectra when the accretion rate is low, probably arising from Comptonization of cyclo-synchrotron photons from the corona, and a soft power law which dominates at higher accretion rates, arising from Comptonization of seed photons from the accretion disc.

  18. Efficient multiple time scale method for modeling compressible vapor plume dynamics inside transient keyhole during fiber laser welding

    Science.gov (United States)

    Pang, Shengyong; Chen, Xin; Li, Wen; Shao, Xinyu; Gong, Shuili

    2016-03-01

    Efficient coupling modeling of multiple time scale interactions between keyhole, weld pool and compressible vapor plume during laser welding has long been limited. To address this problem, we present a highly efficient multiple time scale method combining a novel dual-time stepping and Ghost Fluid interpolation strategy with incompressible and compressible fluid solvers, which allows us predicting the compressible plume dynamics inside transient keyhole in fiber laser welding for the first time. In our method, the compressible dynamic vapor inside the transient keyhole is solved with a Roe scheme based algorithm and the incompressible molten liquid of weld pool is calculated by a Projection method. A novel temperature dependent boundary condition of vapor plume is also proposed for the consideration of the dynamic evaporation phenomena on the transient keyhole wall. It is found that the time dependent distributions of vapor plume characteristics, including temperature, pressure, velocity, density and Mach number distributions inside the transient keyhole induced by laser welding can be reasonably predicted by comparing to experimental and literature data. It is also shown that the proposed multiple time scale method is around 60 times faster than the vapor plume modeling method using a single nanosecond scale time step. For the vapor plume in a typical fiber laser welding process, the results indicate that the peak pressure can be greater than 2.0 atmospheric pressures; the average density is around 0.15-0.3 kg/m3 which is much smaller than the air density; and the local Mach number can be greater than 0.8 or even 1.0 Mach which demonstrates the necessity to treat the vapor plume as a compressible fluid.

  19. A General Systems Theory for Chaos, Quantum Mechanics and Gravity for Dynamical Systems of all Space-Time Scales

    OpenAIRE

    Selvam, A. M.

    2005-01-01

    Non-local connections, i. e. long-range space-time correlations intrinsic to the observed subatomic dynamics of quantum systems is also exhibited by macro-scale dynamical systems as selfsimilar fractal space-time fluctuations and is identified as self-organized criticality. The author has developed a general systems theory for the observed self-organized criticality applicable to dynamical systems of all space-time scales based on the concept that spatial integration of enclosed small-scale f...

  20. Life in the Frequency Domain: the Biological Impacts of Changes in Climate Variability at Multiple Time Scales.

    Science.gov (United States)

    Dillon, Michael E; Woods, H Arthur; Wang, George; Fey, Samuel B; Vasseur, David A; Telemeco, Rory S; Marshall, Katie; Pincebourde, Sylvain

    2016-07-01

    Over the last few decades, biologists have made substantial progress in understanding relationships between changing climates and organism performance. Much of this work has focused on temperature because it is the best kept of climatic records, in many locations it is predicted to keep rising into the future, and it has profound effects on the physiology, performance, and ecology of organisms, especially ectothermic organisms which make up the vast majority of life on Earth. Nevertheless, much of the existing literature on temperature-organism interactions relies on mean temperatures. In reality, most organisms do not directly experience mean temperatures; rather, they experience variation in temperature over many time scales, from seconds to years. We propose to shift the focus more directly on patterns of temperature variation, rather than on means per se, and present a framework both for analyzing temporal patterns of temperature variation and for incorporating those patterns into predictions about organismal biology. In particular, we advocate using the Fourier transform to decompose temperature time series into their component sinusoids, thus allowing transformations between the time and frequency domains. This approach provides (1) standardized ways of visualizing the contributions that different frequencies make to total temporal variation; (2) the ability to assess how patterns of temperature variation have changed over the past half century and may change into the future; and (3) clear approaches to manipulating temporal time series to ask "what if" questions about the potential effects of future climates. We first summarize global patterns of change in temperature variation over the past 40 years; we find meaningful changes in variation at the half day to yearly times scales. We then demonstrate the utility of the Fourier framework by exploring how power added to different frequencies alters the overall incidence of long-term waves of high and low

  1. Studies of Ocean Predictability at Decade to Century Time Scales Using a Global Ocean General Circulation Model in a Parallel Computing Environment

    Energy Technology Data Exchange (ETDEWEB)

    Barnett, T.P.

    1998-11-30

    The objectives of this report are to determine the structure of oceanic natural variability at time scales of decades to centuries, characterize the physical mechanisms responsible for the variability; determine the relative importance of heat, fresh water, and moment fluxes on the variability; determine the predictability of the variability on these times scales. (B204)

  2. Dynamics of condensate formation in stochastic transport with pair-factorized steady states: Nucleation and coarsening time scales

    Science.gov (United States)

    Nagel, Hannes; Janke, Wolfhard

    2016-05-01

    Driven diffusive systems such as the zero-range process (ZRP) and the pair-factorized steady states (PFSS) stochastic transport process are versatile tools that lend themselves to the study of transport phenomena on a generic level. While their mathematical structure is simple enough to allow significant analytical treatment, they offer a variety of interesting phenomena. With appropriate dynamics, the ZRP and PFSS models feature a condensation transition where, for a supercritical density, the translational symmetry breaks spontaneously and excess particles form a single-site or spatially extended condensate, respectively. In this paper we numerically study the typical time scales of the two stages of this condensation process: Nucleation and coarsening. Nucleation is the first stage of condensation where the bulk system relaxes to its stationary distribution and droplet nuclei form in the system. These droplets then gradually grow or evaporate in the coarsening regime to coalesce in a single condensate when the system finally relaxes to the stationary state. We use the ZRP condensation model to discuss the choice of the estimation method for the nucleation time scale and present scaling exponents for the ZRP and PFSS condensation models with respect to the choice of the typical droplet nuclei mass. We then proceed to present scaling exponents in the coarsening regime of the ZRP for partially asymmetric dynamics and the PFSS model for symmetric and asymmetric dynamics.

  3. Dispersive and mixing characteristics for turbulent porous media flows based on local length and time scale measurements

    Science.gov (United States)

    Liburdy, James; Patil, Vishal

    2012-11-01

    Porous media flows have a very wide range of applications, both in engineering applications and natural flows. Local mixing and dispersion is strongly influenced by the complex pore geometry. Understanding mixing properties requires knowledge of the range of scales present within the flow and how they vary with Reynolds number. Experiments have been conducted using time resolved two component PIV based on refractive index matching of the solid and liquid phases. The flow characteristics vary over a large range of Reynolds numbers, typically based on an average pore velocity and hydraulic diameter or bead size as the characteristic length. In this study we examine the effect of increased pore Reynolds number on the turbulence characteristics for Reynolds numbers from approximately 400 to 4000. In particular the integral and Kolmogorov length scales are estimated, along with the determination of the integral velocity and Eulerian time scales. These are then used to estimate the Lagrangian time scale. The asymptotic behavior associated with increasing pore Reynolds number is shown, and used to evaluate the scaling relationships. Results are also used to demonstrate the evaluation of the mechanical dispersion coefficient and that it compares well with results obtained using global methods such as solute breakthrough curves. Funding by NSF grant 0933857, Particulate and Multiphase Processing.

  4. Multiple Time-scale Characteristics of Runoff Variations in Middle Reaches of Huolin River and Their Effects

    Institute of Scientific and Technical Information of China (English)

    LU Xiaoning; DENG Wei; ZHANG Shuqing

    2007-01-01

    The Huolin River is one of the most important water sources for Xianghai wetland, Horqin wetland, and Chaganhu wetland in the western Songnen Plain of Northeast China. The annual runoff series of 46 years at Baiyunhushuo Hydrologic Station, which is located in the middle reaches of the Huolin River, were analyzed by using wavelet analysis. Main objective was to discuss the periodic characteristics of the runoff, and examine the temporal patterns of the Huolin River recharging to the floodplain wetlands in the lower reaches of the river, and the corresponding effects of recharging variation on the environmental evolution of the wetlands. The results show that the annual runoff varied mainly at three time scales. The intensities of periodical signals at different time scales were strongly characterized by local distribution in its time frequency domain. The interdecadal variation at a scale of more than 30yr played a leading role in the temporal pattern of runoff variation, and at this scale, the runoff at Baiyunhushuo Hydrologic Station varied in turn of flood, draught and flood. Accordingly, the landscape of the floodplain wetlands presented periodic features, especially prominent before the 1990s. Compared with intense human activities, the runoff periodic pattern at middle (10-20yr) and small (l-10yr) scales, which has relatively low energy, exerted unobvious effects on the environmental evolution of the floodplain wetlands, especially after the 1990s.

  5. AE index forecast at different time scales through an ANN algorithm based on L1 IMF and plasma measurements

    Science.gov (United States)

    Pallocchia, G.; Amata, E.; Consolini, G.; Marcucci, M. F.; Bertello, I.

    2008-02-01

    The AE index is known to have two main components, one directly driven by the solar wind and the other related to the magnetotail unloading process. As regards the role played by the IMF and solar wind parameters, recently several authors used artificial neural networks (ANN) to forecast AE from solar wind data. Following this track, in this paper we present a study of the AE forecast at different time scales, from 5 min to 1 h, in order to check whether the performance of the ANN prediction varies significantly as a function of the AE time resolution.The study is based on a new ANN Elman network with Bz (in GSM) and Vx as inputs, one hidden layer containing four neurons, four context units and one output neuron. We find that the forecast AE values, during disturbed AE periods, result to be always smaller than the experimental values; on the other hand, the algorithm performance improves as the time scale increases, i.e. the total standard deviation (calculated over a test data set) between the forecast and the Kyoto AE decreases as the averaging time increases. Under the hypothesis that this decrease follows an exponential law, we find that the 1 h scale normalised standard deviation is 0.975, very close to the asymptotic value of 0.95 for an infinite averaging time. We interpret our results in the sense that the unloading component of the AE variations cannot be predicted from IMF and solar wind parameters only.

  6. Climate impact of beef: an analysis considering multiple time scales and production methods without use of global warming potentials

    Science.gov (United States)

    Pierrehumbert, R. T.; Eshel, G.

    2015-08-01

    An analysis of the climate impact of various forms of beef production is carried out, with a particular eye to the comparison between systems relying primarily on grasses grown in pasture (‘grass-fed’ or ‘pastured’ beef) and systems involving substantial use of manufactured feed requiring significant external inputs in the form of synthetic fertilizer and mechanized agriculture (‘feedlot’ beef). The climate impact is evaluated without employing metrics such as {{CO}}2{{e}} or global warming potentials. The analysis evaluates the impact at all time scales out to 1000 years. It is concluded that certain forms of pastured beef production have substantially lower climate impact than feedlot systems. However, pastured systems that require significant synthetic fertilization, inputs from supplemental feed, or deforestation to create pasture, have substantially greater climate impact at all time scales than the feedlot and dairy-associated systems analyzed. Even the best pastured system analyzed has enough climate impact to justify efforts to limit future growth of beef production, which in any event would be necessary if climate and other ecological concerns were met by a transition to primarily pasture-based systems. Alternate mitigation options are discussed, but barring unforseen technological breakthroughs worldwide consumption at current North American per capita rates appears incompatible with a 2 °C warming target.

  7. A robust computational technique for model order reduction of two-time-scale discrete systems via genetic algorithms.

    Science.gov (United States)

    Alsmadi, Othman M K; Abo-Hammour, Zaer S

    2015-01-01

    A robust computational technique for model order reduction (MOR) of multi-time-scale discrete systems (single input single output (SISO) and multi-input multioutput (MIMO)) is presented in this paper. This work is motivated by the singular perturbation of multi-time-scale systems where some specific dynamics may not have significant influence on the overall system behavior. The new approach is proposed using genetic algorithms (GA) with the advantage of obtaining a reduced order model, maintaining the exact dominant dynamics in the reduced order, and minimizing the steady state error. The reduction process is performed by obtaining an upper triangular transformed matrix of the system state matrix defined in state space representation along with the elements of B, C, and D matrices. The GA computational procedure is based on maximizing the fitness function corresponding to the response deviation between the full and reduced order models. The proposed computational intelligence MOR method is compared to recently published work on MOR techniques where simulation results show the potential and advantages of the new approach.

  8. Climate response to changes in atmospheric carbon dioxide and solar irradiance on the time scale of days to weeks

    Science.gov (United States)

    Cao, Long; Bala, Govindasamy; Caldeira, Ken

    2012-09-01

    Recent studies show that fast climate response on time scales of less than a month can have important implications for long-term climate change. In this study, we investigate climate response on the time scale of days to weeks to a step-function quadrupling of atmospheric CO2 and contrast this with the response to a 4% increase in solar irradiance. Our simulations show that significant climate effects occur within days of a stepwise increase in both atmospheric CO2 content and solar irradiance. Over ocean, increased atmospheric CO2 warms the lower troposphere more than the surface, increasing atmospheric stability, moistening the boundary layer, and suppressing evaporation and precipitation. In contrast, over ocean, increased solar irradiance warms the lower troposphere to a much lesser extent, causing a much smaller change in evaporation and precipitation. Over land, both increased CO2 and increased solar irradiance cause rapid surface warming that tends to increase both evaporation and precipitation. However, the physiological effect of increased atmospheric CO2 on plant stomata reduces plant transpiration, drying the boundary layer and decreasing precipitation. This effect does not occur with increased solar irradiance. Therefore, differences in climatic effects from CO2 versus solar forcing are manifested within days after the forcing is imposed.

  9. Time scaling relations for step bunches from models with step-step attractions (B1-type models)

    Science.gov (United States)

    Krasteva, A.; Popova, H.; Akutsu, N.; Tonchev, V.

    2016-03-01

    The step bunching instability is studied in three models of step motion defined in terms of ordinary differential equations (ODE). The source of instability in these models is step-step attraction, it is opposed by step-step repulsion and the developing surface patterns reflect the balance between the two. The first model, TE2, is a generalization of the seminal model of Tersoff et al. (1995). The second one, LW2, is obtained from the model of Liu and Weeks (1998) using the repulsions term to construct the attractions one with retained possibility to change the parameters in the two independently. The third model, MM2, is a minimal one constructed ad hoc and in this article it plays a central role. New scheme for scaling the ODE in vicinal studies is applied towards deciphering the pre-factors in the time-scaling relations. In all these models the patterned surface is self-similar - only one length scale is necessary to describe its evolution (hence B1-type). The bunches form finite angles with the terraces. Integrating numerically the equations for step motion and changing systematically the parameters we obtain the overall dependence of time-scaling exponent β on the power of step-step attractions p as β = 1/(3+p) for MM2 and hypothesize based on restricted set of data that it is β = 1/(5+p) for LW2 and TE2.

  10. Short-time-scale variability in ventilation and export productivity during the formation of Mediterranean sapropel S1

    Science.gov (United States)

    Jilbert, Tom; Reichart, Gert-Jan; Mason, Paul; de Lange, Gert J.

    High-resolution laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) scanning of laminated sediments from the Urania basin is used to investigate short-time-scale variability in export productivity and redox conditions during the formation of eastern Mediterranean sapropel S1. Sedimentary enrichments of molybdenum (Mo), vanadium (V), and uranium (U) reflect deep-water redox conditions, most likely those near to the seawater-brine interface, while enrichment of Ba is related to biogenic barite and hence to export productivity. The enrichments of all four elements show strong variability on multidecadal to multicentennial time scales throughout S1. A partial decoupling of export productivity from redox conditions at the height of sapropel formation suggests that hydrographic changes, i.e., a variable ventilation rate of the eastern Mediterranean, played an important role in determining deep-water redox conditions. A pronounced switch is observed in the enrichments of redox-sensitive trace metals, from dominantly 300-600 year variability during early S1 to dominantly 100-300 year variability during late S1, indicating a change in the mean frequency of variability in the ventilation rate. The presence of a similar shift in the frequency of tropical and extratropical climate records at this time suggests that ventilation of the eastern Mediterranean was coupled to global climate variability.

  11. Plant signalling peptides

    OpenAIRE

    Wiśniewska, Justyna; Trejgell, Alina; Tretyn, Andrzej

    2003-01-01

    Biochemical and genetic studies have identified peptides that play crucial roles in plant growth and development, including defence mechanisms in response to wounding by pests, the control of cell division and expansion, and pollen self-incompatibility. The first two signalling peptides to be described in plants were tomato systemin and phytosulfokine (PSK). There is also biochemical evidence that natriuretic peptide-like molecules, immunologically-relatedt o those found ...

  12. Time scales in LISA

    CERN Document Server

    Pireaux, S

    2007-01-01

    The LISA mission is a space interferometer aiming at the detection of gravitational waves in the [$10^{-4}$,$10^{-1}$] Hz frequency band. In order to reach the gravitational wave detection level, a Time Delay Interferometry (TDI) method must be applied to get rid of (most of) the laser frequency noise and optical bench noise. This TDI analysis is carried out in terms of the coordinate time corresponding to the Barycentric Coordinate Reference System (BCRS), TCB, whereas the data at each of the three LISA stations is recorded in terms of each station proper time. We provide here the required proper time versus BCRS time transformation. We show that the difference in rate of station proper time versus TCB is of the order of $5 10^{-8}$. The difference between station proper times and TCB exhibits an oscillatory trend with a maximum amplitude of about $10^{-3}$ s.

  13. Polycyclic peptide therapeutics.

    Science.gov (United States)

    Baeriswyl, Vanessa; Heinis, Christian

    2013-03-01

    Owing to their excellent binding properties, high stability, and low off-target toxicity, polycyclic peptides are an attractive molecule format for the development of therapeutics. Currently, only a handful of polycyclic peptides are used in the clinic; examples include the antibiotic vancomycin, the anticancer drugs actinomycin D and romidepsin, and the analgesic agent ziconotide. All clinically used polycyclic peptide drugs are derived from natural sources, such as soil bacteria in the case of vancomycin, actinomycin D and romidepsin, or the venom of a fish-hunting coil snail in the case of ziconotide. Unfortunately, nature provides peptide macrocyclic ligands for only a small fraction of therapeutic targets. For the generation of ligands of targets of choice, researchers have inserted artificial binding sites into natural polycyclic peptide scaffolds, such as cystine knot proteins, using rational design or directed evolution approaches. More recently, large combinatorial libraries of genetically encoded bicyclic peptides have been generated de novo and screened by phage display. In this Minireview, the properties of existing polycyclic peptide drugs are discussed and related to their interesting molecular architectures. Furthermore, technologies that allow the development of unnatural polycyclic peptide ligands are discussed. Recent application of these technologies has generated promising results, suggesting that polycyclic peptide therapeutics could potentially be developed for a broad range of diseases. PMID:23355488

  14. Liquid Chromatography on chip

    OpenAIRE

    Faure, Karine

    2010-01-01

    Abstract Liquid chromatography is one of the most powerful separation techniques as illustrated by its leading role in analytical sciences through both academic and industrial communities. Its implementation in microsystems appears to be crucial in the development of ?TAS. If electrophoretic techniques have been widely used in miniaturized devices, liquid chromatography has faced multiple challenges in the downsizing process. During the past five years significant breakthroughs...

  15. Gas chromatography in space

    Science.gov (United States)

    Akapo, S. O.; Dimandja, J. M.; Kojiro, D. R.; Valentin, J. R.; Carle, G. C.

    1999-01-01

    Gas chromatography has proven to be a very useful analytical technique for in situ analysis of extraterrestrial environments as demonstrated by its successful operation on spacecraft missions to Mars and Venus. The technique is also one of the six scientific instruments aboard the Huygens probe to explore Titan's atmosphere and surface. A review of gas chromatography in previous space missions and some recent developments in the current environment of fiscal constraints and payload size limitations are presented.

  16. A method to fast position the trigger time scale of non-cable seismographs based on controllable vibrator

    Science.gov (United States)

    Zheng, F.; Lin, J.; Chen, Z.; Zhang, L.; Huaizhu, Z.; Yang, H.

    2011-12-01

    The data transmission part constitutes the core technology of a non-cable seismograph, whose performance determines the applicability of the entire system. Due to its long collecting duration and high sampling rate, a non-cable seismograph can store a relatively large amount of original seismic data. Yet only a small proportion of the recovered data are effective while the majority of them are ineffective and redundant. High redundancy of data undermines the efficiency of data transmission and processing, so the key to solving the problem comes down to how to precisely determine the triggering time scale and quickly position the effective seismic data. The premise for recovering and processing seismic data in a centralized way is that the clock must be strictly synchronous. The GPS clock synchronization method can synchronize all non-cable seismographs precisely, however, because we are relying on the sync calibration of GPS second pulse signals, under the current condition, we can only get time information as accurate as seconds. This leads to the problem that at a high sampling rate, there could be variations of up to thousands of sampling points when determining the initial sampling point at the triggering moment. To solve this problem, the article proposed an improvement on the vibroseis, adding a vibrator control box and a wireless data transmission module. Figure 1 is a schematic diagram of the vibrator part. The control box comprises the scan signal controller, amplifier, seismic data recorder and GPS controller. The accelerometers are used to collect reference seismic signals and are mounted near the base board and the ground, respectively. As the core component of the vibrator control-box, the GPS controller comprises a high precision crystal oscillator, microsecond counter, GPS unit, scan duration memory, microsecond memory, second memory and data transmitter. It synchronizes GPS clocks, obtains and calculates time information in seconds and microseconds

  17. Photoperiod Regulates vgf-Derived Peptide Processing in Siberian Hamsters.

    Science.gov (United States)

    Noli, Barbara; Brancia, Carla; Pilleri, Roberta; D'Amato, Filomena; Messana, Irene; Manconi, Barbara; Ebling, Francis J P; Ferri, Gian-Luca; Cocco, Cristina

    2015-01-01

    VGF mRNA is induced in specific hypothalamic areas of the Siberian hamster upon exposure to short photoperiods, which is associated with a seasonal decrease in appetite and weight loss. Processing of VGF generates multiple bioactive peptides, so the objective of this study was to determine the profile of the VGF-derived peptides in the brain, pituitary and plasma from Siberian hamsters, and to establish whether differential processing might occur in the short day lean state versus long day fat. Antisera against short sequences at the C- or N- termini of proVGF, as well as against NERP-1, TPGH and TLQP peptides, were used for analyses of tissues, and both immunohistochemistry and enzyme linked immunosorbent assay (ELISA) coupled with high-performance liquid (HPLC) or gel chromatography were carried out. VGF peptide immunoreactivity was found within cortex cholinergic perikarya, in multiple hypothalamic nuclei, including those containing vasopressin, and in pituitary gonadotrophs. ELISA revealed that exposure to short day photoperiod led to a down-regulation of VGF immunoreactivity in the cortex, and a less pronounced decrease in the hypothalamus and pituitary, while the plasma VGF levels were not affected by the photoperiod. HPLC and gel chromatography both confirmed the presence of multiple VGF-derived peptides in these tissues, while gel chromatography showed the presence of the VGF precursor in all tissues tested except for the cortex. These observations are consistent with the view that VGF-derived peptides have pleiotropic actions related to changing photoperiod, possibly by regulating cholinergic systems in the cortex, vasopressin hypothalamic pathways, and the reproductive axis.

  18. Photoperiod Regulates vgf-Derived Peptide Processing in Siberian Hamsters.

    Directory of Open Access Journals (Sweden)

    Barbara Noli

    Full Text Available VGF mRNA is induced in specific hypothalamic areas of the Siberian hamster upon exposure to short photoperiods, which is associated with a seasonal decrease in appetite and weight loss. Processing of VGF generates multiple bioactive peptides, so the objective of this study was to determine the profile of the VGF-derived peptides in the brain, pituitary and plasma from Siberian hamsters, and to establish whether differential processing might occur in the short day lean state versus long day fat. Antisera against short sequences at the C- or N- termini of proVGF, as well as against NERP-1, TPGH and TLQP peptides, were used for analyses of tissues, and both immunohistochemistry and enzyme linked immunosorbent assay (ELISA coupled with high-performance liquid (HPLC or gel chromatography were carried out. VGF peptide immunoreactivity was found within cortex cholinergic perikarya, in multiple hypothalamic nuclei, including those containing vasopressin, and in pituitary gonadotrophs. ELISA revealed that exposure to short day photoperiod led to a down-regulation of VGF immunoreactivity in the cortex, and a less pronounced decrease in the hypothalamus and pituitary, while the plasma VGF levels were not affected by the photoperiod. HPLC and gel chromatography both confirmed the presence of multiple VGF-derived peptides in these tissues, while gel chromatography showed the presence of the VGF precursor in all tissues tested except for the cortex. These observations are consistent with the view that VGF-derived peptides have pleiotropic actions related to changing photoperiod, possibly by regulating cholinergic systems in the cortex, vasopressin hypothalamic pathways, and the reproductive axis.

  19. Insulin C-peptide test

    Science.gov (United States)

    C-peptide ... the test depends on the reason for the C-peptide measurement. Ask your health care provider if ... C-peptide is measured to tell the difference between insulin the body produces and insulin someone injects ...

  20. New theoretical approaches to atomic and molecular dynamics triggered by ultrashort light pulses on the atto- to picosecond time scale

    Energy Technology Data Exchange (ETDEWEB)

    Pabst, Stefan Ulf

    2013-04-15

    The concept of atoms as the building blocks of matter has existed for over 3000 years. A revolution in the understanding and the description of atoms and molecules has occurred in the last century with the birth of quantum mechanics. After the electronic structure was understood, interest in studying the dynamics of electrons, atoms, and molecules increased. However, time-resolved investigations of these ultrafast processes were not possible until recently. The typical time scale of atomic and molecular processes is in the picosecond to attosecond realm. Tremendous technological progress in recent years makes it possible to generate light pulses on these time scales. With such ultrashort pulses, atomic and molecular dynamics can be triggered, watched, and controlled. Simultaneously, the need rises for theoretical models describing the underlying mechanisms. This doctoral thesis focuses on the development of theoretical models which can be used to study the dynamical behavior of electrons, atoms, and molecules in the presence of ultrashort light pulses. Several examples are discussed illustrating how light pulses can trigger and control electronic, atomic, and molecular motions. In the first part of this work, I focus on the rotational motion of asymmetric molecules, which happens on picosecond and femtosecond time scales. Here, the aim is to align all three axes of the molecule as well as possible. To investigate theoretically alignment dynamics, I developed a program that can describe alignment motion ranging from the impulsive to the adiabatic regime. The asymmetric molecule SO{sub 2} is taken as an example to discuss strategies of optimizing 3D alignment without the presence of an external field (i.e., field-free alignment). Field-free alignment is particularly advantageous because subsequent experiments on the aligned molecule are not perturbed by the aligning light pulse. Wellaligned molecules in the gas phase are suitable for diffraction experiments. From the

  1. Towards a Seamless Framework for Drought Analysis and Prediction from Seasonal to Climate Change Time Scales (Plinius Medal Lecture)

    Science.gov (United States)

    Sheffield, Justin

    2013-04-01

    Droughts arguably cause the most impacts of all natural hazards in terms of the number of people affected and the long-term economic costs and ecosystem stresses. Recent droughts worldwide have caused humanitarian and economic problems such as food insecurity across the Horn of Africa, agricultural economic losses across the central US and loss of livelihoods in rural western India. The prospect of future increases in drought severity and duration driven by projected changes in precipitation patterns and increasing temperatures is worrisome. Some evidence for climate change impacts on drought is already being seen for some regions, such as the Mediterranean and east Africa. Mitigation of the impacts of drought requires advance warning of developing conditions and enactment of drought plans to reduce vulnerability. A key element of this is a drought early warning system that at its heart is the capability to monitor evolving hydrological conditions and water resources storage, and provide reliable and robust predictions out to several months, as well as the capacity to act on this information. At longer time scales, planning and policy-making need to consider the potential impacts of climate change and its impact on drought risk, and do this within the context of natural climate variability, which is likely to dominate any climate change signal over the next few decades. There are several challenges that need to be met to advance our capability to provide both early warning at seasonal time scales and risk assessment under climate change, regionally and globally. Advancing our understanding of drought predictability and risk requires knowledge of drought at all time scales. This includes understanding of past drought occurrence, from the paleoclimate record to the recent past, and understanding of drought mechanisms, from initiation, through persistence to recovery and translation of this understanding to predictive models. Current approaches to monitoring and

  2. New theoretical approaches to atomic and molecular dynamics triggered by ultrashort light pulses on the atto- to picosecond time scale

    International Nuclear Information System (INIS)

    The concept of atoms as the building blocks of matter has existed for over 3000 years. A revolution in the understanding and the description of atoms and molecules has occurred in the last century with the birth of quantum mechanics. After the electronic structure was understood, interest in studying the dynamics of electrons, atoms, and molecules increased. However, time-resolved investigations of these ultrafast processes were not possible until recently. The typical time scale of atomic and molecular processes is in the picosecond to attosecond realm. Tremendous technological progress in recent years makes it possible to generate light pulses on these time scales. With such ultrashort pulses, atomic and molecular dynamics can be triggered, watched, and controlled. Simultaneously, the need rises for theoretical models describing the underlying mechanisms. This doctoral thesis focuses on the development of theoretical models which can be used to study the dynamical behavior of electrons, atoms, and molecules in the presence of ultrashort light pulses. Several examples are discussed illustrating how light pulses can trigger and control electronic, atomic, and molecular motions. In the first part of this work, I focus on the rotational motion of asymmetric molecules, which happens on picosecond and femtosecond time scales. Here, the aim is to align all three axes of the molecule as well as possible. To investigate theoretically alignment dynamics, I developed a program that can describe alignment motion ranging from the impulsive to the adiabatic regime. The asymmetric molecule SO2 is taken as an example to discuss strategies of optimizing 3D alignment without the presence of an external field (i.e., field-free alignment). Field-free alignment is particularly advantageous because subsequent experiments on the aligned molecule are not perturbed by the aligning light pulse. Wellaligned molecules in the gas phase are suitable for diffraction experiments. From the

  3. Several Existence Theorems of Multiple Positive Solutions of Nonlinear -Point BVP for an Increasing Homeomorphism and Homomorphism on Time Scales

    Directory of Open Access Journals (Sweden)

    Kang Shugui

    2009-01-01

    Full Text Available Abstract By using fixed point theorems in cones, the existence of multiple positive solutions is considered for nonlinear -point boundary value problem for the following second-order boundary value problem on time scales , , , , where is an increasing homeomorphism and homomorphism and . Some new results are obtained for the existence of twin or an arbitrary odd number of positive solutions of the above problem by applying Avery-Henderson and Leggett-Williams fixed point theorems, respectively. In particular, our criteria generalize and improve some known results by Ma and Castaneda (2001. We must point out for readers that there is only the -Laplacian case for increasing homeomorphism and homomorphism. As an application, one example to demonstrate our results is given.

  4. Prediction of peptide drift time in ion mobility mass spectrometry from sequence-based features

    KAUST Repository

    Wang, Bing

    2013-05-09

    Background: Ion mobility-mass spectrometry (IMMS), an analytical technique which combines the features of ion mobility spectrometry (IMS) and mass spectrometry (MS), can rapidly separates ions on a millisecond time-scale. IMMS becomes a powerful tool to analyzing complex mixtures, especially for the analysis of peptides in proteomics. The high-throughput nature of this technique provides a challenge for the identification of peptides in complex biological samples. As an important parameter, peptide drift time can be used for enhancing downstream data analysis in IMMS-based proteomics.Results: In this paper, a model is presented based on least square support vectors regression (LS-SVR) method to predict peptide ion drift time in IMMS from the sequence-based features of peptide. Four descriptors were extracted from peptide sequence to represent peptide ions by a 34-component vector. The parameters of LS-SVR were selected by a grid searching strategy, and a 10-fold cross-validation approach was employed for the model training and testing. Our proposed method was tested on three datasets with different charge states. The high prediction performance achieve demonstrate the effectiveness and efficiency of the prediction model.Conclusions: Our proposed LS-SVR model can predict peptide drift time from sequence information in relative high prediction accuracy by a test on a dataset of 595 peptides. This work can enhance the confidence of protein identification by combining with current protein searching techniques. 2013 Wang et al.; licensee BioMed Central Ltd.

  5. Probing the Time Scale of FPOP (Fast Photochemical Oxidation of Proteins): Radical Reactions Extend Over Tens of Milliseconds

    Science.gov (United States)

    Vahidi, Siavash; Konermann, Lars

    2016-07-01

    Hydroxyl radical (ṡOH) labeling with mass spectrometry detection reports on protein conformations and interactions. Fast photochemical oxidation of proteins (FPOP) involves ṡOH production via H2O2 photolysis by UV laser pulses inside a flow tube. The experiments are conducted in the presence of a scavenger (usually glutamine) that shortens the ṡOH lifetime. The literature claims that FPOP takes place within 1 μs. This ultrafast time scale implies that FPOP should be immune to labeling-induced artifacts that may be encountered with other techniques. Surprisingly, the FPOP time scale has never been validated in direct kinetic measurements. Here we employ flash photolysis for probing oxidation processes under typical FPOP conditions. Bleaching of the reporter dye cyanine-5 (Cy5) served as readout of the time-dependent radical milieu. Surprisingly, Cy5 oxidation extends over tens of milliseconds. This time range is four orders of magnitude longer than expected from the FPOP literature. We demonstrate that the glutamine scavenger generates metastable secondary radicals in the FPOP solution, and that these radicals lengthen the time frame of Cy5 oxidation. Cy5 and similar dyes are widely used for monitoring the radical dose experienced by proteins in solution. The measured Cy5 kinetics thus strongly suggest that protein oxidation in FPOP extends over a much longer time window than previously thought (i.e., many milliseconds instead of one microsecond). The optical approach developed here should be suitable for assessing the performance of future FPOP-like techniques with improved temporal labeling characteristics.

  6. The effect of model complexity in simulating unsaturated zone flow processes on recharge estimation at varying time scales

    Science.gov (United States)

    Ghasemizade, Mehdi; Moeck, Christian; Schirmer, Mario

    2015-10-01

    Recent increases in computational power have led to the development of more advanced physically-based models which can handle a wide range of environmental processes. Although these models are very useful for increasing our understanding of unsaturated zone flow processes, their outputs usually contain high uncertainty, particularly when the level of complexity is not supported by observations. In this context, the aim of this paper is to compare the performance of three different model conceptualizations of a shallow unsaturated soil zone using the physically-based model HydroGeoSphere (HGS). To accomplish this task, we simulated actual evapotranspiration (ET), water content (WC) and discharge (D) from a weighing lysimeter for each of the conceptual models. Conceptual Model 1 considers the lysimeter as a homogeneous zone with matrix flow, while Conceptual Model 2 has an added preferential flow component. Conceptual Model 3 includes layered heterogeneity in addition to the matrix and preferential flow components. The results indicated that the model performance in reproducing daily ET, WC and D improves when we move from simple models to more complex models. A comparison between event-based, monthly, seasonal and yearly time scales indicates that the simplest conceptual model is not reliable for reproducing event-based discharges. However, it can compete with more complex models at annual scales, although the uncertainty bound for the simple model is very high. While increasing complexity from the simplest to the more complex model leads to lower uncertainty bounds and more reliable values of the lysimeter discharge at monthly and seasonal time scales, uncertainty bounds became larger when complexity increased in the most complex model. This is related to a higher number of unknown model parameters in the calibration which are not supported by the available observation datasets.

  7. Probing the Time Scale of FPOP (Fast Photochemical Oxidation of Proteins): Radical Reactions Extend Over Tens of Milliseconds.

    Science.gov (United States)

    Vahidi, Siavash; Konermann, Lars

    2016-07-01

    Hydroxyl radical (⋅OH) labeling with mass spectrometry detection reports on protein conformations and interactions. Fast photochemical oxidation of proteins (FPOP) involves ⋅OH production via H2O2 photolysis by UV laser pulses inside a flow tube. The experiments are conducted in the presence of a scavenger (usually glutamine) that shortens the ⋅OH lifetime. The literature claims that FPOP takes place within 1 μs. This ultrafast time scale implies that FPOP should be immune to labeling-induced artifacts that may be encountered with other techniques. Surprisingly, the FPOP time scale has never been validated in direct kinetic measurements. Here we employ flash photolysis for probing oxidation processes under typical FPOP conditions. Bleaching of the reporter dye cyanine-5 (Cy5) served as readout of the time-dependent radical milieu. Surprisingly, Cy5 oxidation extends over tens of milliseconds. This time range is four orders of magnitude longer than expected from the FPOP literature. We demonstrate that the glutamine scavenger generates metastable secondary radicals in the FPOP solution, and that these radicals lengthen the time frame of Cy5 oxidation. Cy5 and similar dyes are widely used for monitoring the radical dose experienced by proteins in solution. The measured Cy5 kinetics thus strongly suggest that protein oxidation in FPOP extends over a much longer time window than previously thought (i.e., many milliseconds instead of one microsecond). The optical approach developed here should be suitable for assessing the performance of future FPOP-like techniques with improved temporal labeling characteristics. Graphical Abstract ᅟ. PMID:27067899

  8. Towards generation of bioactive peptides from meat industry waste proteins: Generation of peptides using commercial microbial proteases.

    Science.gov (United States)

    Ryder, Kate; Bekhit, Alaa El-Din; McConnell, Michelle; Carne, Alan

    2016-10-01

    Five commercially available food-grade microbial protease preparations were evaluated for their ability to hydrolyse meat myofibrillar and connective tissue protein extracts to produce bioactive peptides. A bacterial-derived protease (HT) extensively hydrolysed both meat protein extracts, producing peptide hydrolysates with significant in vitro antioxidant and ACE inhibitor activities. The hydrolysates retained bioactivity after simulated gastrointestinal hydrolysis challenge. Gel permeation chromatography sub-fractionation of the crude protein hydrolysates showed that the smaller peptide fractions exhibited the highest antioxidant and ACE inhibitor activities. OFFGEL electrophoresis of the small peptides of both hydrolysates showed that low isoelectric point peptides had antioxidant activity; however, no consistent relationship was observed between isoelectric point and ACE inhibition. Cell-based assays indicated that the hydrolysates present no significant cytotoxicity towards Vero cells. The results indicate that HT protease hydrolysis of meat myofibrillar and connective tissue protein extracts produces bioactive peptides that are non-cytotoxic, should be stable in the gastrointestinal tract and may contain novel bioactive peptide sequences. PMID:27132822

  9. Peptide Nucleic Acids

    DEFF Research Database (Denmark)

    2004-01-01

    A novel class of compounds known as peptide nucleic acids, bind complementary DNA and RNA strands, and generally do so more strongly than the corresponding DNA or RNA strands while exhibiting increased sequence specificity and solubility. The peptide nucleic acids comprise ligands selected from...

  10. CTHRSSVVC Peptide as a Possible Early Molecular Imaging Target for Atherosclerosis

    Science.gov (United States)

    Silva, Rosemeire A.; Giordano, Ricardo J.; Gutierrez, Paulo S.; Rocha, Viviane Z.; Rudnicki, Martina; Kee, Patrick; Abdalla, Dulcinéia S. P.; Puech-Leão, Pedro; Caramelli, Bruno; Arap, Wadih; Pasqualini, Renata; Meneghetti, José C.; Marques, Fabio L. N.; Khoobchandani, Menka; Katti, Kattesh V.; Lugão, Ademar B.; Kalil, Jorge

    2016-01-01

    The purpose of our work was to select phages displaying peptides capable of binding to vascular markers present in human atheroma, and validate their capacity to target the vascular markers in vitro and in low-density lipoprotein receptor knockout (LDLr−/−) mouse model of atherosclerosis. By peptide fingerprinting on human atherosclerotic tissues, we selected and isolated four different peptides sequences, which bind to atherosclerotic lesions and share significant similarity to known human proteins with prominent roles in atherosclerosis. The CTHRSSVVC-phage peptide displayed the strongest reactivity with human carotid atherosclerotic lesions (p 95% yield as determined by high performance liquid chromatography (HPLC), to validate the binding of the peptide in atherosclerotic plaque specimens. The results supported our hypothesis that CTHRSSVVC peptide has a remarkable sequence for the development of theranostics approaches in the treatment of atherosclerosis and other diseases. PMID:27563889

  11. 高效液相色谱-串联质谱法测定养殖环境沉积物中多肽类抗生素残留量%Determination of Peptide Antibiotics Residues in Sediment From Aquaculture Environment by High Performance Liquid Chromatography-Tandem Mass Spectrometry

    Institute of Scientific and Technical Information of China (English)

    钱卓真; 罗冬莲; 罗方方; 叶玫; 汤水粉

    2016-01-01

    A new method for the determination of peptide antibiotics in sediment from aquaculture environment by high performance liquid chromatography-tandem mass spectrometry was developed. The target analytes in sediments were ultrasonically extracted twice with citrate buffer solution and methol mixture (3∶ 4, V/ V), followed by complexation with 0. 5 g of Na2 EDTA, purification with 5 mL of methyl isobutyl ketone, and clean-up with HLB-SPE column. The analytes were separated on a MGII C18 column by gradient elution with 0. 1% formaic acid-0. 1% formaic acid acetonitrile as mobile phase, detected in multiple reaction monitoring (MRM) with electrospray ionization (ESI) under positive ion mode, and quantified by external standard method. The calibration curves were linear (R2 >0. 999) over a concentration range of 10 -10000μg / L for colistin and bacitracin and 4-4000 μg / L for virginiamycin M1 . The limits of detection (S / N = 3) were 5 μg / kg for colistin and bacitracin and 2 μg / kg for virginiamycin M1 . The limits of quantification (S / N=10) was 10 μg / kg for colistin and bacitracin and 4 μg / kg for virginiamycin M1 . At three spiked levels, the recoveries ranged from 79. 7% to 91. 6% (RSD=1. 9% -10. 8% ), showing high sensitivity, good reproducibility and wide applicability.%建立了测定水产养殖环境沉积物中多肽类抗生素残留量的高效液相色谱串联质谱法。沉积物经10 mL甲醇-柠檬酸-Na2 HPO4溶液(3∶4, V/ V)超声提取2次,0.5 g 乙二胺四乙酸二钠络合除杂,5 mL 甲基异丁基甲酮净化,HLB 固相萃取柱进一步富集净化,MGII C18色谱柱分离,0.1%甲酸与0.1%甲酸-乙腈梯度洗脱,ESI+电离,多反应监测模式(MRM)监测,外标法定量。粘菌素和杆菌肽在10~10000μg/ L 范围内,维吉尼霉素 M1在4~4000μg/ L 范围内,线性回归系数均大于0.999,方法检出限为2~5μg/ kg,方法定量限为4~10μg/ kg。在3个浓度添加水平下,多肽类抗生素回收率79.7%~91.6%,

  12. Screening of soy protein-derived hypotriglyceridemic di-peptides in vitro and in vivo

    Directory of Open Access Journals (Sweden)

    Matsui Toshiro

    2011-05-01

    Full Text Available Abstract Background Soy protein and soy peptides have attracted considerable attention because of their potentially beneficial biological properties, including antihypertensive, anticarcinogenic, and hypolipidemic effects. Although soy protein isolate contains several bioactive peptides that have distinct physiological activities in lipid metabolism, it is not clear which peptide sequences are responsible for the triglyceride (TG-lowering effects. In the present study, we investigated the effects of soy protein-derived peptides on lipid metabolism, especially TG metabolism, in HepG2 cells and obese Otsuka Long-Evans Tokushima fatty (OLETF rats. Results In the first experiment, we found that soy crude peptide (SCP-LD3, which was prepared by hydrolyze of soy protein isolate with endo-type protease, showed hypolipidemic effects in HepG2 cells and OLETF rats. In the second experiment, we found that hydrophilic fraction, separated from SCP-LD3 with hydrophobic synthetic absorbent, revealed lipid-lowering effects in HepG2 cells and OLETF rats. In the third experiment, we found that Fraction-C (Frc-C peptides, fractionated from hydrophilic peptides by gel permeation chromatography-high performance liquid chromatography, significantly reduced TG synthesis and apolipoprotein B (apoB secretion in HepG2 cells. In the fourth experiment, we found that the fraction with 0.1% trifluoroacetic acid, isolated from Frc-C peptides by octadecylsilyl column chromatography, showed hypolipidemic effects in HepG2 cells. In the final experiment, we found that 3 di-peptides, Lys-Ala, Val-Lys, and Ser-Tyr, reduced TG synthesis, and Ser-Tyr additionally reduced apoB secretion in HepG2 cells. Conclusion Novel active peptides with TG-lowering effects from soy protein have been isolated.

  13. Alpha-amidated peptides derived from pro-opiomelanocortin in human pituitary tumours

    DEFF Research Database (Denmark)

    Fenger, M; Johnsen, A H

    1988-01-01

    Human pituitary tumours, obtained at surgery for Cushing's disease and Nelson's syndrome, were extracted and the content and molecular forms of pro-opiomelanocortin (POMC)-derived peptides determined by radioimmunoassay, gel chromatography, reversed-phase high-performance liquid chromatography....... In conclusion, all the molecular forms of the amidated peptides detected in tumours from patients with Cushing's disease and Nelson's syndrome were similar to the molecular forms found in the normal human pituitary. The main difference between the tumours and the normal pituitary was the greater amount...... (HPLC) and sequence analysis. In the tumours from patients with Cushing's disease the mean concentrations of amidated peptides relative to the total amount of POMC were as follows: alpha-MSH, 1.7%; amidated gamma-MSH (gamma 1-MSH), 8.5% and the peptide linking gamma-MSH and ACTH in the precursor (hinge...

  14. Variability in net ecosystem exchange from hourly to inter-annual time scales at adjacent pine and hardwood forests: a wavelet analysis.

    Science.gov (United States)

    Stoy, Paul C; Katul, Gabriel G; Siqueira, Mario B S; Juang, Jehn-Yih; McCarthy, Heather R; Kim, Hyun-Seok; Oishi, A Christopher; Oren, Ram

    2005-07-01

    Orthonormal wavelet transformation (OWT) is a computationally efficient technique for quantifying underlying frequencies in nonstationary and gap-infested time series, such as eddy-covariance-measured net ecosystem exchange of CO2 (NEE). We employed OWT to analyze the frequency characteristics of synchronously measured and modeled NEE at adjacent pine (PP) and hardwood (HW) ecosystems. Wavelet cospectral analysis showed that NEE at PP was more correlated to light and vapor pressure deficit at the daily time scale, and NEE at HW was more correlated to leaf area index (LAI) and temperature, especially soil temperature, at seasonal time scales. Models were required to disentangle the impacts of environmental drivers on the components of NEE, ecosystem carbon assimilation (Ac) and ecosystem respiration (RE). Sensitivity analyses revealed that using air temperature rather than soil temperature in RE models improved the modeled wavelet spectral frequency response on time scales longer than 1 day at both ecosystems. Including LAI improved RE model fit on seasonal time scales at HW, and incorporating parameter variability improved the RE model response at annual time scales at both ecosystems. Resolving variability in canopy conductance, rather than leaf-internal CO2, was more important for modeling Ac at both ecosystems. The PP ecosystem was more sensitive to hydrologic variables that regulate canopy conductance: vapor pressure deficit on weekly time scales and soil moisture on seasonal to interannual time scales. The HW ecosystem was sensitive to water limitation on weekly time scales. A combination of intrinsic drought sensitivity and non-conservative water use at PP was the basis for this response. At both ecosystems, incorporating variability in LAI was required for an accurate spectral representation of modeled NEE. However, nonlinearities imposed by canopy light attenuation were of little importance to spectral fit. The OWT revealed similarities and differences in

  15. Mapping molecular flexibility of spin labeled proteins on the nanosecond and longer time scales via CW lineshape analysis and osmolyte-perturbation EPR

    OpenAIRE

    López, Carlos Javier

    2012-01-01

    Proteins in solution exhibit structural fluctuations on a wide range of characteristic time scales, from fast backbone motions in the picosecond to nanosecond range to slow conformational exchange in the microsecond to millisecond time domain. These motions can play important roles in function, thus elucidation of molecular mechanisms underlying function requires experimental techniques capable of measuring motions on these time scales. Site directed spin labeling and EPR spectroscopy (SDSL-...

  16. Truncated glucagon-like peptide I, an insulin-releasing hormone from the distal gut

    DEFF Research Database (Denmark)

    Holst, J J; Orskov, C; Nielsen, O V;

    1987-01-01

    By hydrophobic gel permeation and high pressure liquid chromatography we isolated from pig intestinal mucosa a peptide which corresponds to proglucagon 78-107 as suggested by chromatography and determination of its N-terminal sequence. Natural and synthetic proglucagon 78-107 dose dependently and...... and potently increased insulin secretion from the isolated perfused pig pancreas. Proglucagon 78-107 also secreted by the small intestine may participate in the hormonal control of insulin secretion.......By hydrophobic gel permeation and high pressure liquid chromatography we isolated from pig intestinal mucosa a peptide which corresponds to proglucagon 78-107 as suggested by chromatography and determination of its N-terminal sequence. Natural and synthetic proglucagon 78-107 dose dependently...

  17. Two novel antimicrobial peptides purified from the symbiotic bacteria Xenorhabdus budapestensis NMC-10.

    Science.gov (United States)

    Xiao, Yao; Meng, Fanlu; Qiu, Dewen; Yang, Xiufen

    2012-06-01

    Symbiotic bacteria, which are carried in the intestinal vesicle of the infective stage of juvenile entomopathogenic nematodes, produce broad-spectrum antibiotics. In this study, we aimed to isolate the antimicrobial peptides from the culture of the entomopathogenic bacterium Xenorhabdus budapestensis NMC-10. By screening chromatography columns and optimizing flow rate, pH, salinity and other purification conditions, we identified the final purification procedures which consisted of Q ion-exchange chromatography, gel filtration chromatography and two-step reverse-phase chromatography. Two novel antimicrobial peptides were identified via Q-TOF-TOF and de novo sequencing, and designated as GP-19 and EP-20. Both natural and synthetic peptides demonstrated broad-spectrum antimicrobial activities. The synthetic GP-19 peptide was active against Verticillium dahlia with EC(50) values of 17.54 μg/ml and highly inhibited the growth of a variety of bacteria, while the synthetic EP-20 peptide was highly active against Phytophthora capsici with EC(50) values of 3.14 μg/ml. PMID:22497806

  18. Implications of variability on many time scales for scientific advice on sustainable management of living marine resources

    Science.gov (United States)

    Rice, Jake

    The conceptual basis for understanding and management of living marine resources is built on three basic ecological principles developed in the first half of the past century: the law of the minimum, competitive exclusion, and succession. This paper highlights aspects of these principles that make them insufficient as a sound foundation for understanding and managing marine ecosystems, points out dangers of continuing to use approaches built on them, and presents alternatives which might be more appropriate and of lower risk. To do this, the paper considers variability of marine ecosystems on annual, medium and long-term time scales, highlighting that these scales correspond to less than, approximately equal to, and much greater than, the generation times of dominant predators in the systems. It also considers how each interval of variability may affect directly ecosystems which are controlled from the bottom up, top down, and middle outward, and how position and duration of forcing affect five types of responses: growth, maturation, recruitment, predation, and competition. Generally these five processes have manifestations at the scale of individuals, populations, and ecosystems, attention is drawn to which manifestations are the most significant for each duration and position of forcing. Effects of some combinations of duration of forcing and position of forcing can be explained reasonably well by conventional ecological theory. For other combinations, particularly forcing at time scales of predator generations on top-down or middle-out ecosystems, theory based on contest competition and equilibria are likely to be misleading. In these systems the major dynamics are transients, when many ecosystems are far from their carrying capacities, so scramble competition dominates, and the carrying capacity is not helpful in explaining the system dynamics. This review clarifies the sorts of questions that we should be asking, in order to begin to understand the transient

  19. Size fractionation by slalom chromatography and hydrodynamic chromatography

    OpenAIRE

    Ricardo P. Dias

    2008-01-01

    Hydrodynamic chromatography, also called separation by flow, is based on the use of the parabolic flow profile occurring in open capillaries or in the pores from a column filled with non-porous particles. The hydrodynamic chromatography separation medium, if any, is much simpler than that from size exclusion chromatography (porous particles), the former technique being used in the size-fractionation of many colloids and macromolecules. The transition between hydrodynamic chromatography (obtai...

  20. A liver metalloendopeptidase which degrades the circulating hypotensive peptide hormones bradykinin and atrial natriuretic peptide

    Directory of Open Access Journals (Sweden)

    Carvalho K.M.

    1999-01-01

    Full Text Available A new metalloendopeptidase was purified to apparent homogeneity from a homogenate of normal human liver using successive steps of chromatography on DEAE-cellulose, hydroxyapatite and Sephacryl S-200. The purified enzyme hydrolyzed the Pro7-Phe8 bond of bradykinin and the Ser25-Tyr26 bond of atrial natriuretic peptide. No cleavage was produced in other peptide hormones such as vasopressin, oxytocin or Met- and Leu-enkephalin. This enzyme activity was inhibited by 1 mM divalent cation chelators such as EDTA, EGTA and o-phenanthroline and was insensitive to 1 µM phosphoramidon and captopril, specific inhibitors of neutral endopeptidase (EC 3.4.24.11 and angiotensin-converting enzyme (EC 3.4.15.1, respectively. With Mr 85 kDa, the enzyme exhibits optimal activity at pH 7.5. The high affinity of this endopeptidase for bradykinin (Km = 10 µM and for atrial natriuretic peptide (Km = 5 µM suggests that it may play a physiological role in the inactivation of these circulating hypotensive peptide hormones.