Sample records for chromatography time-scale peptide

  1. Towards Liquid Chromatography Time-Scale Peptide Sequencing and Characterization of Post-Translational Modifications in the Negative-Ion Mode Using Electron Detachment Dissociation Tandem Mass Spectrometry

    DEFF Research Database (Denmark)

    Kjeldsen, Frank; Hørning, Ole B; Jensen, Søren S


    Electron detachment dissociation (EDD) of peptide poly-anions is gentle towards post-translational modifications (PTMs) and produces predictable and interpretable fragment ion types (a., x ions). However, EDD is considered an inefficient fragmentation technique and has not yet been implemented...... coverage) were sequenced by both EDD and ECD. The relative small overlap of identified BSA peptides demonstrates the complementarity of the two dissociation modes. Phosphopeptide mixtures from three trypsin-digested phosphoproteins were subjected to LC-EDD-MS/MS resulting in the identification of five...

  2. Affinity Purification of Insulin by Peptide-Ligand Affinity Chromatography

    Institute of Scientific and Technical Information of China (English)


    The affinity heptapeptide (HWWWPAS) for insulin, selected from phage display library,was coupled to EAH Sepharose 4B gel and packed to a 1-mL column. The column was used for the affinity purification of insulin from protein mixture and commercial insulin preparation. It was observed that the minor impurity in the commercial insulin was removed by the affinity chromatography. Nearly 40 mg of insulin could be purified with the 1-mL affinity column. The results revealed the high specificity and capacity of the affinity column for insulin purification. Moreover, based on the analysis of the amino acids in the peptide sequence, shorter peptides were designed and synthesized for insulin chromatography. As a result, HWWPS was found to be a good alternative to HWWWPAS, while the other two peptides with three or four amino acids showed weak affinity for insulin. The results indicated that the peptide sequence of HWWWPAS was quite conservative for specific binding of insulin.

  3. Diagonal reverse-phase chromatography applications in peptide-centric proteomics: ahead of catalogue-omics? (United States)

    Gevaert, Kris; Van Damme, Petra; Martens, Lennart; Vandekerckhove, Joël


    Diagonal electrophoresis/chromatography was described 40 years ago and was used to isolate specific sets of peptides from simple peptide mixtures such as protease digests of purified proteins. Recently, we have adapted the core technology of diagonal chromatography so that the technique can be used in so-called gel-free, peptide-centric proteome studies. Here we review the different procedures we have developed over the past few years, sorting of methionyl, cysteinyl, amino terminal, and phosphorylated peptides. We illustrate the power of the technique, termed COFRADIC (combined fractional diagonal chromatography), in the case of a peptide-centric analysis of a sputum sol phase sample of a patient suffering from chronic obstructive pulmonary disease (COPD). We were able to identify an unexpectedly high number of intracellular proteins next to known biomarkers.

  4. Irreversibility time scale. (United States)

    Gallavotti, G


    Entropy creation rate is introduced for a system interacting with thermostats (i.e., for a system subject to internal conservative forces interacting with "external" thermostats via conservative forces) and a fluctuation theorem for it is proved. As an application, a time scale is introduced, to be interpreted as the time over which irreversibility becomes manifest in a process leading from an initial to a final stationary state of a mechanical system in a general nonequilibrium context. The time scale is evaluated in a few examples, including the classical Joule-Thompson process (gas expansion in a vacuum).

  5. Applications of hydrophilic interaction chromatography to amino acids, peptides, and proteins. (United States)

    Periat, Aurélie; Krull, Ira S; Guillarme, Davy


    This review summarizes the recent advances in the analysis of amino acids, peptides, and proteins using hydrophilic interaction chromatography. Various reports demonstrate the successful analysis of amino acids under such conditions. However, a baseline resolution of the 20 natural amino acids has not yet been published and for this reason, there is often a need to use mass spectrometry for detection to further improve selectivity. Hydrophilic interaction chromatography is also recognized as a powerful technique for peptide analysis, and there are a lot of papers showing its applicability for proteomic applications (peptide mapping). It is expected that its use for peptide mapping will continue to grow in the future, particularly because this analytical strategy can be combined with reversed-phase liquid chromatography, in a two-dimensional setup, to reach very high resolving power. Finally, the interest in hydrophilic interaction chromatography for intact proteins analysis is less evident due to possible solubility issues and a lack of suitable hydrophilic interaction chromatography stationary phases. To date, it has been successfully employed only for the characterization of membrane proteins, histones, and the separation of glycosylated isoforms of an intact glycoprotein. From our point of view, the number of hydrophilic interaction chromatography columns compatible with intact proteins (higher upper temperature limit, large pore size, etc.) is still too limited.

  6. Selecting protein N-terminal peptides by combined fractional diagonal chromatography. (United States)

    Staes, An; Impens, Francis; Van Damme, Petra; Ruttens, Bart; Goethals, Marc; Demol, Hans; Timmerman, Evy; Vandekerckhove, Joël; Gevaert, Kris


    In recent years, procedures for selecting the N-terminal peptides of proteins with analysis by mass spectrometry have been established to characterize protease-mediated cleavage and protein α-N-acetylation on a proteomic level. As a pioneering technology, N-terminal combined fractional diagonal chromatography (COFRADIC) has been used in numerous studies in which these protein modifications were investigated. Derivatization of primary amines--which can include stable isotope labeling--occurs before trypsin digestion so that cleavage occurs after arginine residues. Strong cation exchange (SCX) chromatography results in the removal of most of the internal peptides. Diagonal, reversed-phase peptide chromatography, in which the two runs are separated by reaction with 2,4,6-trinitrobenzenesulfonic acid, results in the removal of the C-terminal peptides and remaining internal peptides and the fractionation of the sample. We describe here the fully matured N-terminal COFRADIC protocol as it is currently routinely used, including the most substantial improvements (including treatment with glutamine cyclotransferase and pyroglutamyl aminopeptidase to remove pyroglutamate before SCX, and a sample pooling scheme to reduce the overall number of liquid chromatography-tandem mass spectrometry analyses) that were made since its original publication. Completion of the N-terminal COFRADIC procedure takes ~5 d.

  7. Modeling of mixed-mode chromatography of peptides. (United States)

    Bernardi, Susanna; Gétaz, David; Forrer, Nicola; Morbidelli, Massimo


    Mixed-mode chromatographic materials are more and more often used for the purification of biomolecules, such as peptides and proteins. In many instances they in fact exhibit better selectivity values and therefore improve the purification efficiency compared to classical materials. In this work, a model to describe biomolecules retention in cation-exchange/reversed-phase (CIEX-RP) mixed-mode columns under diluted conditions has been developed. The model accounts for the effect of the salt and organic modifier concentration on the biomolecule Henry coefficient through three parameters: α, β and γ. The α parameter is related to the adsorption strength and ligand density, β represents the number of organic modifier molecules necessary to displace one adsorbed biomolecule and γ represents the number of salt molecules necessary to desorb one biomolecule. The latter parameter is strictly related to the number of charges on the biomolecule surface interacting with the ion-exchange ligands and it is shown experimentally that its value is close to the biomolecule net charge. The model reliability has been validated by a large set of experimental data including retention times of two different peptides (goserelin and insulin) on five columns: a reversed-phase C8 column and four CIEX-RP columns with different percentages of sulfonic groups and various concentration values of the salt and organic modifier. It has been found that the percentage of sulfonic groups on the surface strongly affects the peptides adsorption strength, and in particular, in the cases investigated, a CIEX ligand density around 0.04μmol/m(2) leads to optimal retention values.

  8. Modeling of ion-pairing effect in peptide reversed-phase chromatography. (United States)

    Gétaz, David; Hariharan, Subrahmaniam B; Butté, Alessandro; Morbidelli, Massimo


    The modeling of counterion and organic modifier concentration effects in peptide APIs reversed-phase preparative chromatography is discussed in this manuscript. A stoichiometric retention model based on the counterion binding to the charged functional groups of the peptide is proposed. The model parameters were evaluated using a rather large set of retention data measured in mobile phases with various counterions and acetonitrile concentrations. The model parameters were experimentally validated by a new counterion binding measurement technique. The n(max) model parameter value was found to be equal to the peptide net charge, whereas the K model parameter value was found to be specific to the counterion type (i.e. AcO(-)peptide saturation capacity was also investigated. It was shown that, at low acetonitrile concentration, the peptide saturation capacity was constant for all investigated counterion types and concentrations. On the other hand, at intermediate acetonitrile concentration, the peptide saturation capacity was significantly lower and with a tendency to increase with the counterion concentration. On the whole, the developed model provides a reliable a reliable tool for the design and development of peptide purification processes at the preparative and industrial scale.

  9. Improved recovery of proteome-informative, protein N-terminal peptides by combined fractional diagonal chromatography (COFRADIC). (United States)

    Staes, An; Van Damme, Petra; Helsens, Kenny; Demol, Hans; Vandekerckhove, Joël; Gevaert, Kris


    We previously described a proteome-wide, peptide-centric procedure for sorting protein N-terminal peptides and used these peptides as readouts for protease degradome and xenoproteome studies. This procedure is part of a repertoire of gel-free techniques known as COmbined FRActional DIagonal Chromatography (COFRADIC) and highly enriches for alpha-amino-blocked peptides, including alpha-amino-acetylated protein N-terminal peptides. Here, we introduce two additional steps that significantly increase the fraction of such proteome-informative, N-terminal peptides: strong cation exchange (SCX) segregation of alpha-amino-blocked and alpha-amino-free peptides and an enzymatic step liberating pyroglutamyl peptides for 2,4,6-trinitrobenzenesulphonic acid (TNBS) modification and thus COFRADIC sorting. The SCX step reduces the complexity of the analyte mixture by enriching N-terminal peptides and depleting alpha-amino-free internal peptides as well as proline-starting peptides prior to COFRADIC. The action of pyroglutamyl aminopeptidases prior to the first COFRADIC peptide separation results in greatly diminishing numbers of contaminating pyroglutamyl peptides in peptide maps. We further show that now close to 95% of all COFRADIC-sorted peptides are alpha-amino-acetylated and, using the same amount of starting material, our novel procedure leads to an increased number of protein identifications.

  10. On the Geologic Time Scale

    NARCIS (Netherlands)

    Gradstein, F.M.; Ogg, J.G.; Hilgen, F.J.


    This report summarizes the international divisions and ages in the Geologic Time Scale, published in 2012 (GTS2012). Since 2004, when GTS2004 was detailed, major developments have taken place that directly bear and have considerable impact on the intricate science of geologic time scaling. Precam br

  11. Detection and Identification of Heme c-Modified Peptides by Histidine Affinity Chromatography, High-Performance Liquid Chromatography-Mass Spectrometry, and Database Searching

    Energy Technology Data Exchange (ETDEWEB)

    Merkley, Eric D.; Anderson, Brian J.; Park, Jea H.; Belchik, Sara M.; Shi, Liang; Monroe, Matthew E.; Smith, Richard D.; Lipton, Mary S.


    Multiheme c-type cytochromes (proteins with covalently attached heme c moieties) play important roles in extracellular metal respiration in dissimilatory metal-reducing bacteria. Liquid chromatography-tandem mass spectrometry-(LC-MS/MS) characterization of c-type cytochromes is hindered by the presence of multiple heme groups, since the heme c modified peptides are typically not observed, or if observed, not identified. Using a recently reported histidine affinity chromatography (HAC) procedure, we enriched heme c tryptic peptides from purified bovine heart cytochrome c, a bacterial decaheme cytochrome, and subjected these samples to LC-MS/MS analysis. Enriched bovine cytochrome c samples yielded three- to six-fold more confident peptide-spectrum matches to heme-c containing peptides than unenriched digests. In unenriched digests of the decaheme cytochrome MtoA from Sideroxydans lithotrophicus ES-1, heme c peptides for four of the ten expected sites were observed by LC-MS/MS; following HAC fractionation, peptides covering nine out of ten sites were obtained. Heme c peptide spiked into E. coli lysates at mass ratios as low as 10-4 was detected with good signal-to-noise after HAC and LC-MS/MS analysis. In addition to HAC, we have developed a proteomics database search strategy that takes into account the unique physicochemical properties of heme c peptides. The results suggest that accounting for the double thioether link between heme c and peptide, and the use of the labile heme fragment as a reporter ion, can improve database searching results. The combination of affinity chromatography and heme-specific informatics yielded increases in the number of peptide-spectrum matches of 20-100-fold for bovine cytochrome c.

  12. Detection and identification of heme c-modified peptides by histidine affinity chromatography, high-performance liquid chromatography-mass spectrometry, and database searching. (United States)

    Merkley, Eric D; Anderson, Brian J; Park, Jea; Belchik, Sara M; Shi, Liang; Monroe, Matthew E; Smith, Richard D; Lipton, Mary S


    Multiheme c-type cytochromes (proteins with covalently attached heme c moieties) play important roles in extracellular metal respiration in dissimilatory metal-reducing bacteria. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) characterization of c-type cytochromes is hindered by the presence of multiple heme groups, since the heme c modified peptides are typically not observed or, if observed, not identified. Using a recently reported histidine affinity chromatography (HAC) procedure, we enriched heme c tryptic peptides from purified bovine heart cytochrome c, two bacterial decaheme cytochromes, and subjected these samples to LC-MS/MS analysis. Enriched bovine cytochrome c samples yielded 3- to 6-fold more confident peptide-spectrum matches to heme c containing peptides than unenriched digests. In unenriched digests of the decaheme cytochrome MtoA from Sideroxydans lithotrophicus ES-1, heme c peptides for 4 of the 10 expected sites were observed by LC-MS/MS; following HAC fractionation, peptides covering 9 out of 10 sites were obtained. Heme c peptide spiked into E. coli lysates at mass ratios as low as 1×10(-4) was detected with good signal-to-noise after HAC and LC-MS/MS analysis. In addition to HAC, we have developed a proteomics database search strategy that takes into account the unique physicochemical properties of heme c peptides. The results suggest that accounting for the double thioether link between heme c and peptide, and the use of the labile heme fragment as a reporter ion, can improve database searching results. The combination of affinity chromatography and heme-specific informatics yielded increases in the number of peptide-spectrum matches of 20-100-fold for bovine cytochrome c.

  13. Polystyrene-type resin used for peptide synthesis: application for anion-exchange and affinity chromatography. (United States)

    Carvalho, Regina S H; Ianzer, Danielle A; Malavolta, Luciana; Rodrigues, Mauricio M; Cilli, Eduardo M; Nakaie, Clovis R


    This paper deals with an unusual application for a copolymer of styrene-1% divinylbenzene bearing high amount of aminomethyl groups for anion-exchange and affinity chromatography. The so-called aminomethyl resin (AMR), to date only employed for peptide synthesis, swelled appreciably in water and was used successfully to purify negatively charged peptides. By correlating swelling degree of beads with pH of the media, it was possible to estimate that the AMR amino group pK(a) is approximately 5.5. In addition, the synthesized acetyl-(NANP)3-AMR succeeded in the affinity interaction with large antibody molecules related to malaria transmission and raised previously against this dodecapeptide sequence.

  14. Immunoaffinity purification of peptide hormones prior to liquid chromatography-mass spectrometry in doping controls. (United States)

    Thomas, Andreas; Schänzer, Wilhelm; Delahaut, Philippe; Thevis, Mario


    For most peptide hormones prohibited in elite sports the concentrations in plasma or urine are very low (pg/mL). Accordingly, hyphenated purification and enrichment steps prior to mass spectrometric detection are required to obtain sufficient doping control assays. Immunoaffinity purification in combination with nano-scale liquid chromatography coupled to high resolution/high accuracy mass spectrometry was found to have the potential of providing the necessary sensitivity and unambiguous specificity to produce reliable results. With the presented methodology 12 prohibited peptides (porcine insulin, Novolog, Apidra, Lantus DesB30-32 metabolite, Humalog and human insulin, Synacthen (synthetic ACTH analogue), luteinizing hormone-releasing hormone (LH-RH), growth hormone releasing hormone (GH-RH(1-29)) and CJC-1295 (GH-RH analogue), LongR(3)-IGF-1 and IFG-1) were simultaneously purified from plasma/serum or urine. With limits of detection for each target compound ranging in the low pg/mL level (urine), the method enables the determination of urinary peptides at physiologically relevant concentrations. For each class of peptides an appropriate antibody and a respective internal standard was implemented ensuring robust analysis conditions. Due to the fast and simple sample preparation procedure (∼25 samples per day) and the fact that all materials are commercial available, the implementation of the methodology to laboratories from other analytical fields (forensics, pharmacokinetic sciences, etc.) is enabled.

  15. Determination of theoretical retention times for peptides analyzed by reversed- -phase high-performance liquid chromatography

    Directory of Open Access Journals (Sweden)

    Jerzy Dziuba


    Full Text Available   Background. Peptides are important components of foods mainly due to their biological activity. The basic method of their identification is reversed phase high-performance liquid chromatography coupled with electrospray-ionization mass spectrometry (RP-HPLC-ESI-MS. Retention time (tR prediction in silico is very helpful in analysis of multicomponent peptide mixtures. One of problems associated with RP-HPLC-ESI-MS is deterioration of mass spectra quality by trifluoroacetic acid (TFA. This problem can be avoided through the use of chromatographic columns designed for work with low TFA concentrations in mobile phase. The objective of this study was to determine the correlations between peptide retention times predicted with the use of a program available on-line and experimental retention times obtained using the column working with low TFA concentrations. Material and methods. The set of synthetic peptides and bovine α-lactalbumin fragments (18 peptides was used in the experiment. Theoretical retention times were calculated using Sequence Specific Retention Calculator (SSRC program. The experimental retention times were measured via RP-HPLC-ESI-MS method using column working with low TFA content. The dependence between theoretical and experimental tR was expressed via empirical equations. Results. The best correlation between theoretical and experimental retention times of peptides containing at least four amino acid residues has been obtained when third order polynomial (R² = 0.9536. Prediction quality for di- and tripeptides was significantly lower. The method described can be applied for cysteine-containing peptides although our sample preparation procedure did not include modification of this amino acid, taken into attention by SSRC program. Conclusions. The results of this study validate the usefulness of a third degree polynomial as a simple function describing the correlation between peptide retention times predicted by an on

  16. A Systematical Analysis of Tryptic Peptide Identification with Reverse Phase Liquid Chromatography and Electrospray Ion Trap Mass Spectrometry

    Institute of Scientific and Technical Information of China (English)

    Wei Sun; Shuzhen Wu; Xiaorong Wang; Dexian Zheng; Youhe Gao


    In this study we systematically analyzed the elution condition of tryptic peptides and the characteristics of identified peptides in reverse phase liquid chromatography and electrospray tandem mass spectrometry (RPLC-MS/MS) analysis. Following protein digestion with trypsin, the peptide mixture was analyzed by on-line RPLC-MS/MS. Bovine serum albumin (BSA) was used to optimize acetonitrile (ACN) elution gradient for tryptic peptides, and Cytochrome C was used to retest the gradient and the sensitivity of LC-MS/MS. The characteristics of identified peptides were also analyzed. In our experiments, the suitable ACN gradient is 5%to 30% for tryptic peptide elution and the sensitivity of LC-MS/MS is 50 fmol.Analysis of the tryptic peptides demonstrated that longer (more than 10 amino acids) and multi-charge state (+2,+3) peptides are likely to be identified, and the hydropathicity of the peptides might not be related to whether it is more likely to be identified or not. The number of identified peptides for a protein might be used to estimate its loading amount under the same sample background. Moreover, in this study the identified peptides present three types of redundancy, namely identification, charge, and sequence redundancy, which may repress low abundance protein identification.

  17. Integral equations on time scales

    CERN Document Server

    Georgiev, Svetlin G


    This book offers the reader an overview of recent developments of integral equations on time scales. It also contains elegant analytical and numerical methods. This book is primarily intended for senior undergraduate students and beginning graduate students of engineering and science courses. The students in mathematical and physical sciences will find many sections of direct relevance. The book contains nine chapters and each chapter is pedagogically organized. This book is specially designed for those who wish to understand integral equations on time scales without having extensive mathematical background.

  18. Chromatography. (United States)

    Brantley, L. Reed, Sr.; Demanche, Edna L.; Klemm, E. Barbara; Kyselka, Will; Phillips, Edwin A.; Pottenger, Francis M.; Yamamoto, Karen N.; Young, Donald B.

    This booklet presents some activities on chromatography. Directions for preparing leaf pigment extracts using alcohol are given, and paper chromatography and thin-layer chromatography are described as modifications of the basic principles of chromatography. (KHR)

  19. A Time scales Noether's theorem


    Anerot, Baptiste; Cresson, Jacky; Pierret, Frédéric


    We prove a time scales version of the Noether's theorem relating group of symmetries and conservation laws. Our result extends the continuous version of the Noether's theorem as well as the discrete one and corrects a previous statement of Bartosiewicz and Torres in \\cite{BT}.

  20. Enrichment and Analysis of Nonenzymatically Glycated Peptides: Boronate Affinity Chromatography Coupled with Electron-Transfer Dissociation Mass Spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Qibin; Tang, Ning; Brock, Jonathan W.; Mottaz, Heather M.; Ames, Jennifer M.; Baynes, John; Smith, Richard D.; Metz, Thomas O.


    Non-enzymatic glycation of peptides and proteins by D-glucose has important implications in the pathogenesis of diabetes mellitus, particularly in the development of diabetic complications. However, no effective high-throughput methods exist for identifying proteins containing this low abundance post-translational modification in bottom-up proteomic studies. In this report, phenylboronate affinity chromatography was used in a two-step enrichment scheme to selectively isolate first glycated proteins and then glycated, tryptic peptides from human serum glycated in vitro. Enriched peptides were subsequently analyzed by alternating electron transfer dissociation (ETD) and collision induced dissociation (CID) tandem mass spectrometry. It was observed that ETD fragmentation mode resulted in a significantly higher number of glycated peptide identifications (87.6% of all identified peptides) versus CID mode (17.0% of all identified peptides), when utilizing dual glycation enrichment on both the protein and peptide level. This study illustrates that phenylboronate affinity chromatography coupled with LC-MS/MS with ETD as the fragmentation mode is an efficient approach for analyses of glycated proteins and can have broad applications in studies of diabetes mellitus.

  1. Relative Quantification of Sites of Peptide and Protein Modification Using Size Exclusion Chromatography Coupled with Electron Transfer Dissociation (United States)

    Xie, Boer; Sharp, Joshua S.


    One difficult problem in the analysis of peptide modifications is quantifying isomeric modifications that differ by the position of the amino acid modified. HPLC separation using C18 reverse phase chromatography coupled with electron transfer dissociation (ETD) in tandem mass spectrometry has recently been shown to be able to relatively quantify how much of a given modification occurs at each amino acid position for isomeric mixtures; however, the resolution of reverse phase chromatography greatly complicates quantification of isomeric modifications by ETD because of the chromatographic separation of peptides with identical modifications at different sequence positions. Using peptide oxidation as a model system, we investigated the use of size exclusion chromatography coupled with ETD fragmentation to separate peptide sequences. This approach allows for the benefits of chromatographic separation of peptide sequences while ensuring co-elution of modification isomers for accurate relative quantification of modifications using standard data-dependent acquisitions. Using this method, the relative amount of modification at each amino acid can be accurately measured from single ETD MS/MS spectra in a standard data-dependent acquisition experiment.

  2. Integrable Equations on Time Scales


    Gurses, Metin; Guseinov, Gusein Sh.; Silindir, Burcu


    Integrable systems are usually given in terms of functions of continuous variables (on ${\\mathbb R}$), functions of discrete variables (on ${\\mathbb Z}$) and recently in terms of functions of $q$-variables (on ${\\mathbb K}_{q}$). We formulate the Gel'fand-Dikii (GD) formalism on time scales by using the delta differentiation operator and find more general integrable nonlinear evolutionary equations. In particular they yield integrable equations over integers (difference equations) and over $q...

  3. The Separation and Quantitation of Peptides with and without Oxidation of Methionine and Deamidation of Asparagine Using Hydrophilic Interaction Liquid Chromatography with Mass Spectrometry (HILIC-MS) (United States)

    Badgett, Majors J.; Boyes, Barry; Orlando, Ron


    Peptides with deamidated asparagine residues and oxidized methionine residues are often not resolved sufficiently to allow quantitation of their native and modified forms using reversed phase (RP) chromatography. The accurate quantitation of these modifications is vital in protein biotherapeutic analysis because they can affect a protein's function, activity, and stability. We demonstrate here that hydrophilic interaction liquid chromatography (HILIC) adequately and predictably separates peptides with these modifications from their native counterparts. Furthermore, coefficients describing the extent of the hydrophilicity of these modifications have been derived and were incorporated into a previously made peptide retention prediction model that is capable of predicting the retention times of peptides with and without these modifications.

  4. The role of ion-pairing in peak deformations in overloaded reversed-phase chromatography of peptides. (United States)

    Tarafder, Abhijit; Aumann, Lars; Morbidelli, Massimo


    The paper reports a study on the role of ion-pairing behind peak deformations, e.g. peak splitting and even peak disappearance, during the elution of a peptide at highly overloaded conditions in reversed-phase chromatography. Deformation of component peaks is not uncommon in chromatography. There are reports which discuss their occurrence, but mostly at analytical scale, while their occurrence is quite common also in the preparative scale, as in the case discussed in this work. This paper first describes the conditions leading to peak splitting and peak disappearance of an industrial peptide, then explains the plausible reasons behind such behaviour, and finally with experimental analysis demonstrates the role of ion-pairing in causing such behaviour.

  5. Effect of high pH column regeneration on the separation performances in reversed phase chromatography of peptides. (United States)

    Gétaz, David; Gencoglu, Mumun; Forrer, Nicola; Morbidelli, Massimo


    Caustic regeneration procedures are often used in chromatographic purification processes of peptides and proteins to remove irreversibly bound impurities from the stationary phase. Silica-based materials are the most commonly used materials in reversed phase chromatography of peptides. Their limited chemical stability at high pH can be, however, problematic when high pH column regeneration (i.e. cleaning in place) is required. The effect of cleaning in place on the surface chemistry of the stationary phase has been investigated using the Tanaka test. It has been shown that the high pH treatment does not significantly affect the hydrophobicity of the material, but it strongly increases its silanol activity. A representative peptide purification process has been used to investigate the impact of cleaning in place on the separation performance. It has been shown that the caustic regeneration increases the peptide retention at high pH (pH 6.5), due to the interactions between the peptide and the negatively charged silanol groups. These unwanted interactions reduce the separation performances by decreasing the selectivity between the late eluting impurities and the main peptide. However, it has been shown that the effect of the silanol groups on the peptide adsorption and on the separation performance can be minimized by carrying out the purification process at low pH (pH approximately 2). In this case, the silanol groups are protonated and their electrostatic interactions with the positively charged analyte (i.e. peptides) are suppressed. In these conditions, the peptide adsorption and the impurity selectivity is not changing upon high pH column regeneration and the separation performance is not affected.

  6. Identification of Nitrotyrosine Containing Peptides using Combined Fractional Diagonal Chromatography (COFRADIC) and Off-Line Nano-LC-MALDI

    DEFF Research Database (Denmark)

    Larsen, Trine R; Bache, Nicolai; Gramsbergen, Jan Bert;


    Protein nitration take place on tyrosine residues under oxidative stress conditions and may influence a number of processes including enzyme activity, protein-protein interactions and phospho-tyrosine signalling pathways. Nitrated proteins have been identified in a number of diseases, however......, the study of these proteins has been compromised by the lack of good methods for identifying nitrated proteins, their nitration sites and the level of nitration. Here, we present a method for identification of nitrated peptides that allows the site specific assignment of nitration, is easy to use...... and reproducible, and opens up for the possibility to quantify the level of nitration of specific peptides as function of different oxidative conditions, namely combined fractional diagonal chromatography (COFRADIC) in combination with off-line nano-LC-MALDI. We identify six nitrated peptides from in vitro...

  7. Inversion of type of separation system in planar chromatography of peptides, using C18 silica-based adsorbents. (United States)

    Gwarda, Radosław Ł; Aletańska-Kozak, Monika; Matosiuk, Dariusz; Dzido, Tadeusz H


    Our previous results show, that C18 silica-based adsorbents used in high-performance thin-layer chromatography (HPTLC), provide complex retention mechanism basing on various polar and nonpolar interactions. Here we present, that in chromatography of peptides, due to mixed-mode properties of these adsorbents, there is a simple way to obtain inversion of separation system type (from reversed-phase, RP, to normal-phase, NP, and vice versa). The results presented provide detailed information how to obtain inversion mentioned and reflect the extent (the type and concentration of organic solvent, the type and concentration of ion-pairing reagent in the mobile phase) of this phenomenon. We show, that the system type inversion results in significant change of selectivity of separation, which may be especially useful in 2D separation of complex samples of basic/amphoteric compounds such as peptides. This results from the fact, that C18 silica-based HPTLC adsorbents, may be used in hydrophilic interaction chromatography (HILIC) or RP chromatography, in dependence on mobile phase composition.

  8. Purification and identification of O-GlcNAc-modified peptides using phosphate-based alkyne CLICK chemistry in combination with titanium dioxide chromatography and mass spectrometry

    DEFF Research Database (Denmark)

    Parker, Benjamin L; Gupta, Pankaj; Cordwell, Stuart


    -containing peptides were enriched using titanium dioxide chromatography. Modified peptides were analyzed using a combination of higher energy collision dissociation for identification and electron transfer dissociation to localize the site of O-GlcNAc attachment. The enrichment method was developed...

  9. Reversed-phase liquid chromatography of radiolabeled peptides using a C18 guard-PAK precolumn system

    Energy Technology Data Exchange (ETDEWEB)

    Carriere, P.D.; Bennett, H.P. (McGill Univ., Montreal, Quebec (Canada))


    In order to avoid radioactive contamination of high-performance liquid chromatography columns and injectors, we have investigated the use of a Guard-PAK precolumn system for the chromatography of ({sup 125}I) labeled peptides. Two gonadotropin-releasing hormone analogs: (1) (D-Ala6-des-Gly10)-GnRH (GnRH-(Ala6)) and (2) (D-Ser(TBu)6-des-Gly10)-GnRH (GnRH-(Ser6)) and rat prolactin (r-PRL) were radiolabeled with {sup 125}I and subjected to reversed-phase liquid chromatography using a C18 Guard-PAK precolumn system. Major peak fractions of purified ({sup 125}I)GnRH-(Ala6), ({sup 125}I)GnRH-(Ser6), and ({sup 125}I)r-PRL eluted at 24%, 28%, and 55% acetonitrile, respectively. Purified ({sup 125}I)GnRH analogs showed specific high affinity binding to rat anterior pituitary gland membranes (specific activity: 1500-1700 Ci/mmol). Purified ({sup 125}I)r-PRL showed high affinity binding to r-PRL antibody by RIA (specific activity: 70-75 microCi/micrograms). This rapid and efficient chromatographic method should be useful in the separation of a wide range of radiolabeled protein and peptide molecules.

  10. Peptide dot immunoassay and immunoblotting: electroblotting from aluminum thin-layer chromatography plates and isoelectric focusing gels to activated nitrocellulose

    DEFF Research Database (Denmark)

    Bjerrum, O.J.; Holm, A.; Lauritzen, Edgar;


    Peptide dot immunoassay, electroblotting, activated nitrocellulose, dot blot, membranes, peptides and proteins......Peptide dot immunoassay, electroblotting, activated nitrocellulose, dot blot, membranes, peptides and proteins...

  11. Quantitation of the immunodominant 33-mer peptide from α-gliadin in wheat flours by liquid chromatography tandem mass spectrometry (United States)

    Schalk, Kathrin; Lang, Christina; Wieser, Herbert; Koehler, Peter; Scherf, Katharina Anne


    Coeliac disease (CD) is triggered by the ingestion of gluten proteins from wheat, rye, and barley. The 33-mer peptide from α2-gliadin has frequently been described as the most important CD-immunogenic sequence within gluten. However, from more than 890 published amino acid sequences of α-gliadins, only 19 sequences contain the 33-mer. In order to make a precise assessment of the importance of the 33-mer, it is necessary to elucidate which wheat species and cultivars contain the peptide and at which concentrations. This paper presents the development of a stable isotope dilution assay followed by liquid chromatography tandem mass spectrometry to quantitate the 33-mer in flours of 23 hexaploid modern and 15 old common (bread) wheat as well as two spelt cultivars. All flours contained the 33-mer peptide at levels ranging from 91–603 μg/g flour. In contrast, the 33-mer was absent (peptide seems to be justified. PMID:28327674

  12. Separation of peptides and intact proteins by electrostatic repulsion reversed phase liquid chromatography. (United States)

    Gritti, Fabrice; Guiochon, Georges


    A new brand of BEH-C18 hybrid particles chemically bonded to a leash carrying an amine group permits the implementation of electrostatic repulsive interactions chromatography. Using columns packed with this material, the influence of the concentration of positive charges bonded to the BEH-C18 surface on the overloaded band profiles of a few positively charged peptides and proteins was investigated in the gradient elution mode. Three columns packed with endcapped BEH-C18 particles bonded with three different surface-charge densities (LOW, MEDIUM and HIGH) were used and compared with those provided by a column packed with non-doped, endcapped BEH-C18 particles. The surface concentrations of fixed charges in the LOW, MEDIUM and HIGH columns were estimated at 0.029, 0.050, and 0.064μmol/m(2), for example, about two orders of magnitude smaller than the surface density of bonded C18 chains (2.1μmol/m(2)). Three different mobile phase additives (0.1% v/v of trifluoro-acetic, phosphoric, and formic acid) were used to optimize the purification levels of proteins under different loading conditions. The weak ion-pairing ions (formate and phosphate) generate smaller retention but broader, more fronting band profiles than those eluted with a stronger ion-pairing ion (trifluoroactate). This effect is worse in the presence of fixed charges at the surface of the BEH-C18 particles. This was explained by an enhanced anti-Langmuirian adsorption behavior of the charged proteins in the presence of fixed surface charges. As the protein concentration increases in the bulk, so does the internal ionic strength, the electrostatic repulsive interactions weaken, and retention increases. Band fronting is mostly eliminated by replacing weak ion-pairing acids with TFA with which the adsorption isotherm remains weakly langmuirian. Faster but still complete gradient separation of insulin and myoglobin were achieved with the HIGH column than with the reference neutral column, despite a measurable

  13. Kalman plus weights: a time scale algorithm (United States)

    Greenhall, C. A.


    KPW is a time scale algorithm that combines Kalman filtering with the basic time scale equation (BTSE). A single Kalman filter that estimates all clocks simultaneously is used to generate the BTSE frequency estimates, while the BTSE weights are inversely proportional to the white FM variances of the clocks. Results from simulated clock ensembles are compared to previous simulation results from other algorithms.

  14. Some integral inequalities on time scales

    Institute of Scientific and Technical Information of China (English)

    Adnan Tuna; Servet Kutukcu


    In this article, we study the reverse Holder type inequality and Holder in-equality in two dimensional case on time scales. We also obtain many integral inequalities by using H(o)lder inequalities on time scales which give Hardy's inequalities as spacial cases.

  15. Stochastic dynamic equations on general time scales

    Directory of Open Access Journals (Sweden)

    Martin Bohner


    Full Text Available In this article, we construct stochastic integral and stochastic differential equations on general time scales. We call these equations stochastic dynamic equations. We provide the existence and uniqueness theorem for solutions of stochastic dynamic equations. The crucial tool of our construction is a result about a connection between the time scales Lebesgue integral and the Lebesgue integral in the common sense.

  16. The Separation and Quantitation of Peptides with and without Oxidation of Methionine and Deamidation of Asparagine Using Hydrophilic Interaction Liquid Chromatography with Mass Spectrometry (HILIC-MS). (United States)

    Badgett, Majors J; Boyes, Barry; Orlando, Ron


    Peptides with deamidated asparagine residues and oxidized methionine residues are often not resolved sufficiently to allow quantitation of their native and modified forms using reversed phase (RP) chromatography. The accurate quantitation of these modifications is vital in protein biotherapeutic analysis because they can affect a protein's function, activity, and stability. We demonstrate here that hydrophilic interaction liquid chromatography (HILIC) adequately and predictably separates peptides with these modifications from their native counterparts. Furthermore, coefficients describing the extent of the hydrophilicity of these modifications have been derived and were incorporated into a previously made peptide retention prediction model that is capable of predicting the retention times of peptides with and without these modifications. Graphical Abstract ᅟ.

  17. Analysis of therapeutic proteins and peptides using multiangle light scattering coupled to ultra high performance liquid chromatography. (United States)

    Espinosa-de la Garza, Carlos E; Miranda-Hernández, Mariana P; Acosta-Flores, Lilia; Pérez, Néstor O; Flores-Ortiz, Luis F; Medina-Rivero, Emilio


    Analysis of the physical properties of biotherapeutic proteins is crucial throughout all the stages of their lifecycle. Herein, we used size-exclusion ultra high performance liquid chromatography coupled to multiangle light scattering and refractive index detection systems to determine the molar mass, mass-average molar mass, molar-mass dispersity and hydrodynamic radius of two monoclonal antibodies (rituximab and trastuzumab), a fusion protein (etanercept), and a synthetic copolymer (glatiramer acetate) employed as models. A customized instrument configuration was set to diminish band-broadening effects and enhance sensitivity throughout detectors. The customized configuration showed a performance improvement with respect to the high-performance liquid chromatography standard configuration, as observed by a 3 h column conditioning and a higher resolution analysis in 20 min. Analysis of the two monoclonal antibodies showed averaged values of 148.0 kDa for mass-average molar mass and 5.4 nm for hydrodynamic radius, whereas for etanercept these values were 124.2 kDa and 6.9 nm, respectively. Molar-mass dispersity was 1.000 on average for these proteins. Regarding glatiramer acetate, a molar mass range from 3 to 45 kDa and a molar-mass dispersity of 1.304 were consistent with its intrinsic peptide diversity, and its mass-average molar mass was 10.4 kDa. Overall, this method demonstrated an accurate determination of molar mass, overcoming the difficulties of size-exclusion chromatography.

  18. Sample displacement chromatography as a method for purification of proteins and peptides from complex mixtures


    Gajdosik, Martina Srajer; Clifton, James; Josic, Djuro


    Sample displacement chromatography (SDC) in reversed-phase and ion-exchange modes was introduced approximately twenty years ago. This method takes advantage of relative binding affinities of components in a sample mixture. During loading, there is a competition among different sample components for the sorption on the surface of the stationary phase. SDC was first used for the preparative purification of proteins. Later, it was demonstrated that this kind of chromatography can also be perform...

  19. Time Scale in Least Square Method

    Directory of Open Access Journals (Sweden)

    Özgür Yeniay


    Full Text Available Study of dynamic equations in time scale is a new area in mathematics. Time scale tries to build a bridge between real numbers and integers. Two derivatives in time scale have been introduced and called as delta and nabla derivative. Delta derivative concept is defined as forward direction, and nabla derivative concept is defined as backward direction. Within the scope of this study, we consider the method of obtaining parameters of regression equation of integer values through time scale. Therefore, we implemented least squares method according to derivative definition of time scale and obtained coefficients related to the model. Here, there exist two coefficients originating from forward and backward jump operators relevant to the same model, which are different from each other. Occurrence of such a situation is equal to total number of values of vertical deviation between regression equations and observation values of forward and backward jump operators divided by two. We also estimated coefficients for the model using ordinary least squares method. As a result, we made an introduction to least squares method on time scale. We think that time scale theory would be a new vision in least square especially when assumptions of linear regression are violated.

  20. Directly coupled high-performance liquid chromatography-accelerator mass spectrometry measurement of chemically modified protein and peptides. (United States)

    Thomas, Avi T; Stewart, Benjamin J; Ognibene, Ted J; Turteltaub, Kenneth W; Bench, Graham


    Quantitation of low-abundance protein modifications involves significant analytical challenges, especially in biologically important applications, such as studying the role of post-translational modifications in biology and measurement of the effects of reactive drug metabolites. (14)C labeling combined with accelerator mass spectrometry (AMS) provides exquisite sensitivity for such experiments. Here, we demonstrate real-time (14)C quantitation of high-performance liquid chromatography (HPLC) separations by liquid sample accelerator mass spectrometry (LS-AMS). By enabling direct HPLC-AMS coupling, LS-AMS overcomes several major limitations of conventional HPLC-AMS, where individual HPLC fractions must be collected and converted to graphite before measurement. To demonstrate LS-AMS and compare the new technology to traditional solid sample AMS (SS-AMS), reduced and native bovine serum albumin (BSA) was modified by (14)C-iodoacetamide, with and without glutathione present, producing adducts on the order of 1 modification in every 10(6) to 10(8) proteins. (14)C incorporated into modified BSA was measured by solid carbon AMS and LS-AMS. BSA peptides were generated by tryptic digestion. Analysis of HPLC-separated peptides was performed in parallel by LS-AMS, fraction collection combined with SS-AMS, and (for peptide identification) electrospray ionization and tandem mass spectrometry (ESI-MS/MS). LS-AMS enabled (14)C quantitation from ng sample sizes and was 100 times more sensitive to (14)C incorporated in HPLC-separated peptides than SS-AMS, resulting in a lower limit of quantitation of 50 zmol (14)C/peak. Additionally, LS-AMS turnaround times were minutes instead of days, and HPLC trace analyses required 1/6th the AMS instrument time required for analysis of graphite fractions by SS-AMS.

  1. The Second Noether Theorem on Time Scales


    Malinowska, Agnieszka B.; Natália Martins


    We extend the second Noether theorem to variational problems on time scales. As corollaries we obtain the classical second Noether theorem, the second Noether theorem for the $h$ -calculus and the second Noether theorem for the $q$ -calculus.

  2. Hardy type inequalities on time scales

    CERN Document Server

    Agarwal, Ravi P; Saker, Samir H


    The book is devoted to dynamic inequalities of Hardy type and extensions and generalizations via convexity on a time scale T. In particular, the book contains the time scale versions of classical Hardy type inequalities, Hardy and Littlewood type inequalities, Hardy-Knopp type inequalities via convexity, Copson type inequalities, Copson-Beesack type inequalities, Liendeler type inequalities, Levinson type inequalities and Pachpatte type inequalities, Bennett type inequalities, Chan type inequalities, and Hardy type inequalities with two different weight functions. These dynamic inequalities contain the classical continuous and discrete inequalities as special cases when T = R and T = N and can be extended to different types of inequalities on different time scales such as T = hN, h > 0, T = qN for q > 1, etc.In this book the authors followed the history and development of these inequalities. Each section in self-contained and one can see the relationship between the time scale versions of the inequalities and...

  3. Peptide sequencing and characterization of post-translational modifications by enhanced ion-charging and liquid chromatography electron-transfer dissociation tandem mass spectrometry

    DEFF Research Database (Denmark)

    Kjeldsen, Frank; Giessing, Anders; Ingrell, Christian R


    We have tested the effect of m-nitrobenzyl alcohol (m-NBA) as a method to increase the average charge state of protonated gas-phase molecular ions generated by ESI from tryptic peptides and phosphopeptides. Various concentrations of m-NBA were added to the mobile phases of a liquid chromatography...

  4. Multivariable dynamic calculus on time scales

    CERN Document Server

    Bohner, Martin


    This book offers the reader an overview of recent developments of multivariable dynamic calculus on time scales, taking readers beyond the traditional calculus texts. Covering topics from parameter-dependent integrals to partial differentiation on time scales, the book’s nine pedagogically oriented chapters provide a pathway to this active area of research that will appeal to students and researchers in mathematics and the physical sciences. The authors present a clear and well-organized treatment of the concept behind the mathematics and solution techniques, including many practical examples and exercises.

  5. Selection of possible marker peptides for the detection of major ruminant milk proteins in food by liquid chromatography-tandem mass spectrometry. (United States)

    Ansari, Parisa; Stoppacher, Norbert; Rudolf, Judith; Schuhmacher, Rainer; Baumgartner, Sabine


    The aim of this work was the determination of peptides, which can function as markers for identification of milk allergens in food samples. Emphasis was placed on two casein proteins (α- and β-casein) and two whey proteins (α-lactalbumin and β-lactoglobulin). In silico tryptic digestion provided preliminary information about the expected peptides. After tryptic digestion of four milk allergens, the analytical data obtained by combination of reversed-phase high performance liquid chromatography and quadrupole tandem mass spectrometry (LC-MS/MS) led to the identification of 26 peptides. Seven of these peptides were synthesized and used for calibration of the LC-MS/MS system. Species specificity of the selected peptides was sought by BLAST search. Among the selected peptides, only LIVTQTMK from β-lactoglobulin (m/z 467.6, charge 2+) was found to be cow milk specific and could function as a marker. Two other peptides, FFVAPFPEVFGK from α-casein (m/z 693.3, charge 2+) and GPFPIIV from β-casein (m/z 742.5, charge 1+), occur in water buffalo milk too. The other four peptides appear in the milk of other species also and can be used as markers for ruminant species milk. Using these seven peptides, a multianalyte MS-based method was developed. For the establishment of the method, it was applied at first to different dairy samples, and then to chocolate and blank samples, and the peptides could be determined down to 1 ng/mL in food samples. At the end, spiked samples were measured, where the target peptides could be detected with a high recovery (over 50%).

  6. Peptide mapping of /sup 125/I-labelled membrane protein of influenza viruses by reverse-phase high-performance liquid chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Darveau, A.; Lecomte, J. (Centre de Recherche en Virologie, Institut Armand-Frappier, Laval, Quebec (Canada)); Seidah, N.G.; Chretien, M. (Institut de Recherches Cliniques de Montreal, Montreal (Canada))


    The resolution potential of reverse-phase high-performance liquid chromatography (HPLC) for peptide analysis of hydrophobic viral membranes has been investigated, using as a model the membrane (M) protein of influenza virus. Proteolytic digests of /sup 125/I-labelled M protein CNBr fragments, extracted from radioiodinated whole virus, have been separated on a uBondapak C/sub 18/ column with an isopropanol or acetonitrile solvent system. Peptide mapping of trypsin digests of M protein from A/PR/8/34 (H1N1) and A/chicken/Germany/N/49 (H10N7) viruses was identical, whereas Staphylococcus aureus V8 protease digests showed minor differences in at least two peptides. The results also show that HPLC is a powerful tool for the separation of proteolytic digests of viral proteins, since the peptide maps are highly reproducible and recovery was always greater than 85%.

  7. Some Nonlinear Integral Inequalities on Time Scales

    Directory of Open Access Journals (Sweden)

    Li Wei Nian


    Full Text Available The purpose of this paper is to investigate some nonlinear integral inequalities on time scales. Our results unify and extend some continuous inequalities and their corresponding discrete analogues. The theoretical results are illustrated by a simple example at the end of this paper.

  8. The Second Noether Theorem on Time Scales

    Directory of Open Access Journals (Sweden)

    Agnieszka B. Malinowska


    Full Text Available We extend the second Noether theorem to variational problems on time scales. As corollaries we obtain the classical second Noether theorem, the second Noether theorem for the h-calculus and the second Noether theorem for the q-calculus.

  9. The second Noether theorem on time scale


    Malinowska, Agnieszka B.; Martins, Natália


    We extend the second Noether theorem to variational problems on time scales. Our result provides as corollaries the classical second Noether theorem, the second Noether theorem for the $h$-calculus and the second Noether theorem for the $q$-calculus.

  10. Some Nonlinear Dynamic Inequalities on Time Scales

    Indian Academy of Sciences (India)

    Wei Nian Li; Weihong Sheng


    The aim of this paper is to investigate some nonlinear dynamic inequalities on time scales, which provide explicit bounds on unknown functions. The inequalities given here unify and extend some inequalities in (B G Pachpatte, On some new inequalities related to a certain inequality arising in the theory of differential equation, J. Math. Anal. Appl. 251 (2000) 736--751).

  11. Identification and quantification of peptides and proteins secreted from prostate epithelial cells by unbiased liquid chromatography tandem mass spectrometry using goodness of fit and analysis of variance. (United States)

    Florentinus, Angelica K; Bowden, Peter; Sardana, Girish; Diamandis, Eleftherios P; Marshall, John G


    The proteins secreted by prostate cancer cells (PC3(AR)6) were separated by strong anion exchange chromatography, digested with trypsin and analyzed by unbiased liquid chromatography tandem mass spectrometry with an ion trap. The spectra were matched to peptides within proteins using a goodness of fit algorithm that showed a low false positive rate. The parent ions for MS/MS were randomly and independently sampled from a log-normal population and therefore could be analyzed by ANOVA. Normal distribution analysis confirmed that the parent and fragment ion intensity distributions were sampled over 99.9% of their range that was above the background noise. Arranging the ion intensity data with the identified peptide and protein sequences in structured query language (SQL) permitted the quantification of ion intensity across treatments, proteins and peptides. The intensity of 101,905 fragment ions from 1421 peptide precursors of 583 peptides from 233 proteins separated over 11 sample treatments were computed together in one ANOVA model using the statistical analysis system (SAS) prior to Tukey-Kramer honestly significant difference (HSD) testing. Thus complex mixtures of proteins were identified and quantified with a high degree of confidence using an ion trap without isotopic labels, multivariate analysis or comparing chromatographic retention times.

  12. Two-step ion-exchange chromatographic purification combined with reversed-phase chromatography to isolate C-peptide for mass spectrometric analysis. (United States)

    Kabytaev, Kuanysh; Durairaj, Anita; Shin, Dmitriy; Rohlfing, Curt L; Connolly, Shawn; Little, Randie R; Stoyanov, Alexander V


    A liquid chromatography with mass spectrometry on-line platform that includes the orthogonal techniques of ion exchange and reversed phase chromatography is applied for C-peptide analysis. Additional improvement is achieved by the subsequent application of cation- and anion-exchange purification steps that allow for isolating components that have their isoelectric points in a narrow pH range before final reversed-phase mass spectrometry analysis. The utility of this approach for isolating fractions in the desired "pI window" for profiling complex mixtures is discussed.

  13. Special Issue on Time Scale Algorithms (United States)


    unclassified Standard Form 298 (Rev. 8-98) Prescribed by ANSI Std Z39-18 IOP PUBLISHING METROLOGIA Metrologia 45 (2008) doi:10.1088/0026-1394/45/6/E01...special issue of Metrologia presents selected papers from the Fifth International Time Scale Algorithm Symposium (VITSAS), including some of the...Paris at the BIPM in 2002 (see Metrologia 40 (3), 2003) • 5th Symposium: in San Fernando, Spain at the ROA in 2008. The early symposia were concerned

  14. Liquidity crises on different time scales (United States)

    Corradi, Francesco; Zaccaria, Andrea; Pietronero, Luciano


    We present an empirical analysis of the microstructure of financial markets and, in particular, of the static and dynamic properties of liquidity. We find that on relatively large time scales (15 min) large price fluctuations are connected to the failure of the subtle mechanism of compensation between the flows of market and limit orders: in other words, the missed revelation of the latent order book breaks the dynamical equilibrium between the flows, triggering the large price jumps. On smaller time scales (30 s), instead, the static depletion of the limit order book is an indicator of an intrinsic fragility of the system, which is related to a strongly nonlinear enhancement of the response. In order to quantify this phenomenon we introduce a measure of the liquidity imbalance present in the book and we show that it is correlated to both the sign and the magnitude of the next price movement. These findings provide a quantitative definition of the effective liquidity, which proves to be strongly dependent on the considered time scales.

  15. Peptides from two sanguinovorous leeches analyzed by ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometric detector

    Directory of Open Access Journals (Sweden)

    Ling Xiao


    Full Text Available Background: Hirudo nipponica Whitman and Poecilobdella manillensis Lesson fall into the family of Hirudinidae Whitman, both of them are sanguinovorous leeches and used a anticoagulant medicines in China. Their medicinal parts are the dried bodies. However, the peptides in the dried body of the two leeches have not been very clear up to now. Objective: To analyze the peptides from two sanguinovorous leeches, H. nipponica and P. manillensis. Materials and Methods: In this article it is reported that the peptides were obtained from anticoagulant active extracted parts of dried bodies of the two leeches and their molecular weights were analyzed by ultra-performance liquid chromatography with electrospray ionization quadrupole time-of-flight mass spectrometry mass spectrometric detector online. Results: Three peptide components were identified from H. nipponica with their molecular weight separately 14998, 15988, and 15956, six peptide components were identified from P. manillensis with molecular weight 9590, 13642, 14998, 17631, 15988, and 16567. Two of peptides from P. manillensis have the same molecular weight 14998 and 15988 as that in H. nipponica. Conclusion: And the two peptides are the main peaks in the base peak ion chromatogram because they occupied a large ratio of total base peak area. Hence the composition of the extracted active part of the two leeches are very close, difference is in that the extract of P. manillensis has more small peptide peaks, but the extract of H. nipponica has not. Furthermore, the tryptic digestion hydrolysates of the extracted active part of each sample were analyzed and the results showed that there were four peaks which only exist in P. manillensis, but not in Hirudo nipponia. They may be the identified peak between the two leeches. This work support the viewpoint that P. manillensis can be used as a medicinal leech as H. nipponia and these peptide components of dried bodies of the two species leeches are a

  16. Immobilized palladium(II) ion affinity chromatography for recovery of recombinant proteins with peptide tags containing histidine and cysteine. (United States)

    Kikot, Pamela; Polat, Aise; Achilli, Estefania; Fernandez Lahore, Marcelo; Grasselli, Mariano


    Fusion of peptide-based tags to recombinant proteins is currently one of the most used tools for protein production. Also, immobilized metal ion affinity chromatography (IMAC) has a huge application in protein purification, especially in research labs. The combination of expression systems of recombinant tagged proteins with this robust chromatographic system has become an efficient and rapid tool to produce milligram-range amounts of proteins. IMAC-Ni(II) columns have become the natural partners of 6xHis-tagged proteins. The Ni(II) ion is considered as the best compromise of selectivity and affinity for purification of a recombinant His-tagged protein. The palladium(II) ion is also able to bind to side chains of amino acids and form ternary complexes with iminodiacetic acid and free amino acids and other sulfur-containing molecules. In this work, we evaluated two different cysteine- and histidine-containing six amino acid tags linked to the N-terminal group of green fluorescent protein (GFP) and studied the adsorption and elution conditions using novel eluents. Both cysteine-containing tagged GFPs were able to bind to IMAC-Pd(II) matrices and eluted successfully using a low concentration of thiourea solution. The IMAC-Ni(II) system reaches less than 20% recovery of the cysteine-containing tagged GFP from a crude homogenate of recombinant Escherichia coli, meanwhile the IMAC-Pd(II) yields a recovery of 45% with a purification factor of 13.

  17. Highly efficient peptide separations in proteomics Part 1. Unidimensional high performance liquid chromatography. (United States)

    Sandra, Koen; Moshir, Mahan; D'hondt, Filip; Verleysen, Katleen; Kas, Koen; Sandra, Pat


    Sample complexity and dynamic range constitute enormous challenges in proteome analysis. The back-end technology in typical proteomics platforms, namely mass spectrometry (MS), can only tolerate a certain complexity, has a limited dynamic range per spectrum and is very sensitive towards ion suppression. Therefore, component overlap has to be minimized for successful mass spectrometric analysis and subsequent protein identification and quantification. The present review describes the advances that have been made in liquid-based separation techniques with focus on the recent developments to boost the resolving power. The review is divided in two parts; the first part deals with unidimensional liquid chromatography and the second part with bi- and multidimensional liquid-based separation techniques. Part 1 mainly focuses on reversed-phase HPLC due to the fact that it is and will, in the near future, remain the technique of choice to be hyphenated with MS. The impact of increasing the column length, decreasing the particle diameter, replacing the traditional packed beds by monolithics, amongst others, is described. The review is complemented with data obtained in the laboratories of the authors.

  18. Large-scale purification of the synthetic peptide fragment 163-171 of human interleukin-beta by multi-dimensional displacement chromatography. (United States)

    Viscomi, G C; Cardinali, C; Longobardi, M G; Verdini, A S


    Multi-dimensional chromatography has been used successfully in the displacement mode for the purification of the synthetic peptide H-Val-Gln-Gly-Glu-Glu-Ser-Asn-Asp-Lys-OH, the fragment 163-171 of human interleukin-beta. This peptide can mimic several of the in vivo and in vitro immunostimulatory activities of the entire protein, except for the inflammatory effect. A large-scale procedure has been developed to purify the synthetic peptide by reversed-phase (RP) and ion-exchange (IE) displacement chromatography (DC) in a single run without any pretreatment. Masses from 100 mg to about 35 g of the unpurified compounds synthesized by a solid-phase technique on a Merrifield-type resin and obtained by acidolytic cleavage from the solid support, can be purified in this way. In the RP-DC mode the carrier and the displacer were aqueous solutions of 0.1% trifluoroacetic acid and 50 mM benzyltributylammonium chloride, respectively, whereas in the IE-DC mode the carrier was water and the displacer 50 mM ammonium citrate solution. RP-DC and IE-DC were also performed in series by directing the effluent of the RP column onto the IE column. Peptide purities and recoveries greater than 96 and 90%, respectively, were obtained.

  19. Prediction of Molar Extinction Coefficients of Proteins and Peptides Using UV Absorption of the Constituent Amino Acids at 214 nm To Enable Quantitative Reverse Phase High-Performance Liquid Chromatography-Mass Spectrometry Analysis

    NARCIS (Netherlands)

    Kuipers, B.J.H.; Gruppen, H.


    The molar extinction coefficients of 20 amino acids and the peptide bond were measured at 214 nm in the presence of acetonitrile and formic acid to enable quantitative comparison of peptides eluting from reversed-phase high-performance liquid chromatography, once identified with mass spectrometry (R

  20. uncertain dynamic systems on time scales

    Directory of Open Access Journals (Sweden)

    V. Lakshmikantham


    Full Text Available A basic feedback control problem is that of obtaining some desired stability property from a system which contains uncertainties due to unknown inputs into the system. Despite such imperfect knowledge in the selected mathematical model, we often seek to devise controllers that will steer the system in a certain required fashion. Various classes of controllers whose design is based on the method of Lyapunov are known for both discrete [4], [10], [15], and continuous [3–9], [11] models described by difference and differential equations, respectively. Recently, a theory for what is known as dynamic systems on time scales has been built which incorporates both continuous and discrete times, namely, time as an arbitrary closed sets of reals, and allows us to handle both systems simultaneously [1], [2], [12], [13]. This theory permits one to get some insight into and better understanding of the subtle differences between discrete and continuous systems. We shall, in this paper, utilize the framework of the theory of dynamic systems on time scales to investigate the stability properties of conditionally invariant sets which are then applied to discuss controlled systems with uncertain elements. For the notion of conditionally invariant set and its stability properties, see [14]. Our results offer a new approach to the problem in question.

  1. Spur in pico-second time scales

    Energy Technology Data Exchange (ETDEWEB)

    Gopinathan, C.; Girija, G. (Bhabha Atomic Research Centre, Bombay (India). Chemistry Div.)


    The spur diffusion model of aqueous radiation chemistry, proposed in 1953, had run into difficulties with the development of pico-second pulse radiolysis in the late 1960s and early seventies. Using the same values for spur parameters, it was impossible to get good agreement with e/sup -/sub(aq) and OH decay in pico and nano second time scales as well as the steady state molecular product yield measurements. This inconsistency was removed by us by assuming that for a given number of dissociations, a number of radii values for the spur are possible, these radii values being related in a gaussian manner. This new approach proved highly successful in getting agreement between the predictions of the spur diffusion model and the pulse radiolysis results as well as the steady state molecular product yield measurements. Our computations have been extended to cover the entire range of spurs from a single dissociation spur to a thirty dissociation spur. Here again agreement with experimental results is good. This approach also gives interesting insights about the spur formation processes in pico and possibly femto second time scales. We have calculated rate constants for the reactions involving the 'precursor' of the hydrated electron with a number of ions.

  2. Separation of small molecular peptides with the same amino acid composition but different sequences by high performance liquid chromatography-electrospray ionization-mass spectrometry

    Institute of Scientific and Technical Information of China (English)


    Peptidomics has emerged as a new discipline in recent years. Mass spectrometry (MS) is the most universal and efficient tool for structure identification of proteins and peptides. However,there is a limitation for the identification of peptides with the same amino acid composition but different se-quences because these peptides have identical mass spectra of molecular ions. This paper presents a high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) method for the separation of small molecular peptides with the same amino acid composition but dif-ferent sequences. Two tripeptides of Gly-Ser-Phe and Gly-Phe-Ser were used as a model sample. The separation behavior has been investigated and the separation conditions have been optimized. Under the optimum conditions,good repeatability was achieved. The developed method could provide a helpful reference for the separation of other peptides with the same amino acid composition but different sequences in the study of proteomics and peptidomics.

  3. Peptide profiling of Internet-obtained Cerebrolysin using high performance liquid chromatography - electrospray ionization ion trap and ultra high performance liquid chromatography - ion mobility - quadrupole time of flight mass spectrometry. (United States)

    Gevaert, Bert; D'Hondt, Matthias; Bracke, Nathalie; Yao, Han; Wynendaele, Evelien; Vissers, Johannes Petrus Cornelis; De Cecco, Martin; Claereboudt, Jan; De Spiegeleer, Bart


    Cerebrolysin, a parenteral peptide preparation produced by controlled digestion of porcine brain proteins, is an approved nootropic medicine in some countries. However, it is also easily and globally available on the Internet. Nevertheless, until now, its exact chemical composition was unknown. Using high performance liquid chromatography (HPLC) coupled to ion trap and ultra high performance liquid chromatography (UHPLC) coupled to quadrupole-ion mobility-time-of-flight mass spectrometry (Q-IM-TOF MS), combined with UniProt pig protein database search and PEAKS de novo sequencing, we identified 638 unique peptides in an Internet-obtained Cerebrolysin sample. The main components in this sample originate from tubulin alpha- and beta-chain, actin, and myelin basic protein. No fragments of known neurotrophic factors like glial cell-derived neurotrophic factor (GDNF), neurotrophin nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF) were found, suggesting that the activities reported in the literature are likely the result of new, hitherto unknown cryptic peptides with nootropic properties.

  4. Discounting in Games across Time Scales

    Directory of Open Access Journals (Sweden)

    Krishnendu Chatterjee


    Full Text Available We introduce two-level discounted games played by two players on a perfect-information stochastic game graph. The upper level game is a discounted game and the lower level game is an undiscounted reachability game. Two-level games model hierarchical and sequential decision making under uncertainty across different time scales. We show the existence of pure memoryless optimal strategies for both players and an ordered field property for such games. We show that if there is only one player (Markov decision processes, then the values can be computed in polynomial time. It follows that whether the value of a player is equal to a given rational constant in two-level discounted games can be decided in NP intersected coNP. We also give an alternate strategy improvement algorithm to compute the value.

  5. Discounting in Games across Time Scales

    CERN Document Server

    Chatterjee, Krishnendu; 10.4204/EPTCS.25.6


    We introduce two-level discounted games played by two players on a perfect-information stochastic game graph. The upper level game is a discounted game and the lower level game is an undiscounted reachability game. Two-level games model hierarchical and sequential decision making under uncertainty across different time scales. We show the existence of pure memoryless optimal strategies for both players and an ordered field property for such games. We show that if there is only one player (Markov decision processes), then the values can be computed in polynomial time. It follows that whether the value of a player is equal to a given rational constant in two-level discounted games can be decided in NP intersected coNP. We also give an alternate strategy improvement algorithm to compute the value.

  6. Extension of gyrokinetics to transport time scales

    CERN Document Server

    Parra, Felix I


    Gyrokinetic simulations have greatly improved our theoretical understanding of turbulent transport in fusion devices. Most gyrokinetic models in use are delta-f simulations in which the slowly varying radial profiles of density and temperature are assumed to be constant for turbulence saturation times, and only the turbulent electromagnetic fluctuations are calculated. New massive simulations are being built to self-consistently determine the radial profiles of density and temperature. However, these new codes have failed to realize that modern gyrokinetic formulations, composed of a gyrokinetic Fokker-Planck equation and a gyrokinetic quasineutrality equation, are only valid for delta-f simulations that do not reach the longer transport time scales necessary to evolve radial profiles. In tokamaks, due to axisymmetry, the evolution of the axisymmetric radial electric field is a challenging problem requiring substantial modifications to gyrokinetic treatments. In this thesis, I study the effect of turbulence o...

  7. Time-Scale Invariant Audio Data Embedding

    Directory of Open Access Journals (Sweden)

    Mansour Mohamed F


    Full Text Available We propose a novel algorithm for high-quality data embedding in audio. The algorithm is based on changing the relative length of the middle segment between two successive maximum and minimum peaks to embed data. Spline interpolation is used to change the lengths. To ensure smooth monotonic behavior between peaks, a hybrid orthogonal and nonorthogonal wavelet decomposition is used prior to data embedding. The possible data embedding rates are between 20 and 30 bps. However, for practical purposes, we use repetition codes, and the effective embedding data rate is around 5 bps. The algorithm is invariant after time-scale modification, time shift, and time cropping. It gives high-quality output and is robust to mp3 compression.

  8. Isolation of cross-linked peptides by diagonal strong cation exchange chromatography for protein complex topology studies by peptide fragment fingerprinting from large sequence databases. (United States)

    Buncherd, Hansuk; Roseboom, Winfried; Ghavim, Behrad; Du, Weina; de Koning, Leo J; de Koster, Chris G; de Jong, Luitzen


    Knowledge of spatial proximity of amino acid residues obtained by chemical cross-linking and mass spectrometric analysis provides information about protein folding, protein-protein interactions and topology of macromolecular assemblies. We show that the use of bis(succinimidyl)-3-azidomethyl glutarate as a cross-linker provides a solution for two major analytical problems of cross-link mapping by peptide fragment fingerprinting (PFF) from complex sequence databases, i.e., low abundance of protease-generated target peptides and lack of knowledge of the masses of linked peptides. Tris(carboxyethyl)phosphine (TCEP) reduces the azido group in cross-linked peptides to an amine group in competition with cleavage of an amide bond formed in the cross-link reaction. TCEP-induced reaction products were separated by diagonal strong cation exchange (SCX) from unmodified peptides. The relation between the sum of the masses of the cleavage products and the mass of the parent cross-linked peptide enables determination of the masses of candidate linked peptides. By reversed phase LC-MS/MS analysis of secondary SCX fractions, we identified several intraprotein and interprotein cross-links in a HeLa cell nuclear extract, aided by software tools supporting PFF from the entire human sequence database. The data provide new information about interacting protein domains, among others from assemblies involved in splicing.

  9. [Determination of peptide and protein diversity in venom of the spider Selenocosmia jiafu by high performance liquid chromatography and mass spectrometry]. (United States)

    Hu, Zhaotun; Xiao, Zhen; Zhou, Xi; Chen, Jia; Chen, Bo; Liu, Zhonghua


    Selenocosmia jiafu (S. jiafu) is recently identified as a new species of spider in P. R. China. These medium bodied venomous spiders are distributed mainly in the hilly areas of southwest of China, mostly at Yunnan and Guangxi Provinces. In order to understand the composition of the S. jiafu venom, we performed a preliminary analysis of this venom using reversed-phase high performance liquid chromatography (RP-HPLC), matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The S. jiafu venom was separated by RP-HPLC in an analytical C18 column (phenomenex 100 A, 250 mm x 4.6 mm, 5 µm) equilibrated with solution A (distilled water with 0.1% trifluoroacetic acid), using a gradient from 0% to 50% of solution B (acetonitrile with 0.1% trifluoroacetic acid) over 50 min with a flow rate of 1 mL/min. The isolated venom proteins were treated with in-gel digestion separated by SDS- PAGE and then identified by liquid chromatography-electrospray ionization quadrupole-time of flight mass spectrometry (LC-ESI-QTOF-MS) techniques. The results show that more than 40 fractions eluted were monitored at 215 nm in the RP-HPLC chromatogram of the venom of the spider S. jiafu. Most of the fractions were eluted with retention times of 5-15 min and 25-40 min, corresponding to 5%-15% and 25%-40% acetonitrile, respectively. The venom contains 238 peptides that follow a bimodal distribution, with about 62.5% of the peptides having a relative molecular mass of 3,000-4,500 and about 33.2% of the peptides having a relative molecular mass of 1,000-3,000. This distribution model is rather different from those of peptides from other tarantula spider venoms analyzed. To explore the relative molecular mass distribution of the venom proteins, the venom was analyzed by SDS-PAGE using standard protocols. Except for peptides with relative molecular mass lower than 10,000, the SDS

  10. EDITORIAL: Special issue on time scale algorithms (United States)

    Matsakis, Demetrios; Tavella, Patrizia


    This special issue of Metrologia presents selected papers from the Fifth International Time Scale Algorithm Symposium (VITSAS), including some of the tutorials presented on the first day. The symposium was attended by 76 persons, from every continent except Antarctica, by students as well as senior scientists, and hosted by the Real Instituto y Observatorio de la Armada (ROA) in San Fernando, Spain, whose staff further enhanced their nation's high reputation for hospitality. Although a timescale can be simply defined as a weighted average of clocks, whose purpose is to measure time better than any individual clock, timescale theory has long been and continues to be a vibrant field of research that has both followed and helped to create advances in the art of timekeeping. There is no perfect timescale algorithm, because every one embodies a compromise involving user needs. Some users wish to generate a constant frequency, perhaps not necessarily one that is well-defined with respect to the definition of a second. Other users might want a clock which is as close to UTC or a particular reference clock as possible, or perhaps wish to minimize the maximum variation from that standard. In contrast to the steered timescales that would be required by those users, other users may need free-running timescales, which are independent of external information. While no algorithm can meet all these needs, every algorithm can benefit from some form of tuning. The optimal tuning, and even the optimal algorithm, can depend on the noise characteristics of the frequency standards, or of their comparison systems, the most precise and accurate of which are currently Two Way Satellite Time and Frequency Transfer (TWSTFT) and GPS carrier phase time transfer. The interest in time scale algorithms and its associated statistical methodology began around 40 years ago when the Allan variance appeared and when the metrological institutions started realizing ensemble atomic time using more than

  11. Identification of bradykinin: related peptides from Phyllomedusa nordestina skin secretion using electrospray ionization tandem mass spectrometry after a single-step liquid chromatography

    Directory of Open Access Journals (Sweden)

    K Conceição


    Full Text Available Amphibian skin secretions are a source of potential new drugs with medical and biotechnological applications. Rich in peptides produced by holocrine-type serous glands in the integument, these secretions play different roles, either in the regulation of physiological skin functions or in the defense against predators or microorganisms. The aim of the present work was to identify novel peptides with bradykinin-like structure and/or activity present in the skin of Phyllomedusa nordestina. In order to achieve this goal, the crude skin secretion of this frog was pre-fractionated by solid phase extraction and separated by reversed-phase chromatography. The fractions were screened for low-molecular-mass peptides and sequenced by mass spectrometry. It was possible to identify three novel bradykinin-related peptides, namely: KPLWRL-NH2 (Pnor 3, RPLSWLPK (Pnor 5 and VPPKGVSM (Pnor 7 presenting vascular activities as assessed by intravital microscopy. Pnor 3 and Pnor 7 were able to induce vasodilation. On the other hand, Pnor 5 was a potent vasoconstrictor. These effects were reproduced by their synthetic analogues.

  12. Liquid chromatography-electrospray linear ion trap mass spectrometry analysis of targeted neuropeptides in Tac1(-/-) mouse spinal cords reveals significant lower concentration of opioid peptides. (United States)

    Saidi, Mouna; Beaudry, Francis


    Tachykinin and opioid peptides play a central role in pain transmission, modulation and inhibition. The treatment of pain is very important in medicine and many studies using NK1 receptor antagonists failed to show significant analgesic effects in humans. Recent investigations suggest that both pronociceptive tachykinins and the analgesic opioid systems are important for normal pain sensation. The analysis of opioid peptides in Tac1(-/-) spinal cord tissues offers a great opportunity to verify the influence of the tachykinin system on specific opioid peptides. The objectives of this study were to develop an HPLC-MS/MRM assay to quantify targeted peptides in spinal cord tissues. Secondly, we wanted to verify if the Tac1(-/-) mouse endogenous opioid system is hampered and therefore affects significantly the pain modulatory pathways. Targeted neuropeptides were analyzed by high performance liquid chromatography linear ion trap mass spectrometry. Our results reveal that EM-2, Leu-Enk and Dyn A were down-regulated in Tac1(-/-) spinal cord tissues. Interestingly, Dyn A was almost 3 fold down-regulated (p<0.0001). No significant concentration differences were observed in mouse Tac1(-/-) spinal cords for Met-Enk and CGRP. The analysis of Tac1(-/-) mouse spinal cords revealed noteworthy decreases of EM-2, Leu-Enk and Dyn A concentrations which strongly suggest a significant impact on the endogenous pain-relieving mechanisms. These observations may have insightful impact on future analgesic drug developments and therapeutic strategies.

  13. Screening and characterization of reactive compounds with in vitro peptide-trapping and liquid chromatography/high-resolution accurate mass spectrometry. (United States)

    Wei, Cong; Chupak, Louis S; Philip, Thomas; Johnson, Benjamin M; Gentles, Robert; Drexler, Dieter M


    The present study describes a novel methodology for the detection of reactive compounds using in vitro peptide-trapping and liquid chromatography-high-resolution accurate mass spectrometry (LC-HRMS). Compounds that contain electrophilic groups can covalently bind to nucleophilic moieties in proteins and form adducts. Such adducts are thought to be associated with drug-mediated toxicity and therefore represent potential liabilities in drug discovery programs. In addition, reactive compounds identified in biological screening can be associated with data that can be misinterpreted if the reactive nature of the compound is not appreciated. In this work, to facilitate the triage of hits from high-throughput screening (HTS), a novel assay was developed to monitor the formation of covalent peptide adducts by compounds suspected to be chemically reactive. The assay consists of in vitro incubations of test compounds (under conditions of physiological pH) with synthetically prepared peptides presenting a variety of nucleophilic moieties such as cysteine, lysine, histidine, arginine, serine, and tyrosine. Reaction mixtures were analyzed using full-scan LC-HRMS, the data were interrogated using postacquisition data mining, and modified amino acids were identified by subsequent LC-HRMS/mass spectrometry. The study demonstrated that in vitro nucleophilic peptide trapping followed by LC-HRMS analysis is a useful approach for screening of intrinsically reactive compounds identified from HTS exercises, which are then removed from follow-up processes, thus obviating the generation of data from biochemical activity assays.

  14. Noether theorem for Birkhoffian systems on time scales (United States)

    Song, Chuan-Jing; Zhang, Yi


    Birkhoff equations on time scales and Noether theorem for Birkhoffian system on time scales are studied. First, some necessary knowledge of calculus on time scales are reviewed. Second, Birkhoff equations on time scales are obtained. Third, the conditions for invariance of Pfaff action and conserved quantities are presented under the special infinitesimal transformations and general infinitesimal transformations, respectively. Fourth, some special cases are given. And finally, an example is given to illustrate the method and results.

  15. Options for rapid analysis of peptides and proteins, using wide-pore, superficially porous, high-performance liquid chromatography particles with unique bonded-phase ligands. (United States)

    Ricker, Robert D; Woodward, Clifford B; Forrer, Kurt; Permar, Bernard J; Chen, Wu


    The large size and complexity of many proteins constrains the reversed-phase high-performance liquid chromatography packings that are useful for their separation. Wide-pore, superficially porous, silica-based packings with solid 4.5-microm cores and a 0.25-microm porous outer layer (Poroshell) demonstrate a variety of characteristics that are beneficial for the separation of proteins. A shorter diffusion distance allows separations of large molecules at high linear velocities. This benefit over totally porous particles is clearly shown using separations of a peptide-protein standard. The structure and reduced surface area (4.5 m2/g) of these superficially porous particles simplifies interactions with its surface, resulting in improved peak shapes and resolution. Specialized bonding chemistries for low- and high-pH operation may be used to change band-spacing and achieve atypical separations. These rapid analysis options are demonstrated using protein standards and very high molecular weight glycosylated proteins including intact monoclonal antibodies, IgM, alpha2-macroglobulin, and glycophorin. In liquid chromatography-mass spectrometry analysis of a myoglobin peptide digest, bidentate-C18-bonded superficially porous packings achieve complete runs in 4 min and demonstrate an elution pattern that is unique from that of material bonded with sterically protected C18 ligands.

  16. A Quaternary Geomagnetic Instability Time Scale (United States)

    Singer, B. S.


    Reversals and excursions of Earth's geomagnetic field create marker horizons that are readily detected in sedimentary and volcanic rocks worldwide. An accurate and precise chronology of these geomagnetic field instabilities is fundamental to understanding several aspects of Quaternary climate, dynamo processes, and surface processes. For example, stratigraphic correlation between marine sediment and polar ice records of climate change across the cryospheres benefits from a highly resolved record of reversals and excursions. The temporal patterns of dynamo behavior may reflect physical interactions between the molten outer core and the solid inner core or lowermost mantle. These interactions may control reversal frequency and shape the weak magnetic fields that arise during successive dynamo instabilities. Moreover, weakening of the axial dipole during reversals and excursions enhances the production of cosmogenic isotopes that are used in sediment and ice core stratigraphy and surface exposure dating. The Geomagnetic Instability Time Scale (GITS) is based on the direct dating of transitional polarity states recorded by lava flows using the 40Ar/39Ar method, in parallel with astrochronologic age models of marine sediments in which O isotope and magnetic records have been obtained. A review of data from Quaternary lava flows and sediments yields a GITS comprising 10 polarity reversals and 27 excursions during the past 2.6 million years. Nine of the ten reversals bounding chrons and subchrons are associated with 40Ar/39Ar ages of transitionally-magnetized lava flows. The tenth, the Guass-Matuyama chron boundary, is tightly bracketed by 40Ar/39Ar dated ash deposits. Of the 27 well-documented excursions, 14 occurred during the Matuyama chron and 13 during the Brunhes chron; 19 have been dated directly using the 40Ar/39Ar method on transitionally-magnetized volcanic rocks and form the backbone of the GITS. Excursions are clearly not the rare phenomena once thought


    Institute of Scientific and Technical Information of China (English)


    The article is concerned with oscillation of nonautonomous neutral dynamic delay equations on time scales. Sufficient conditions are established for the existence of bounded positive solutions and for oscillation of all solutions of this equation. Some results extend known results for difference equations when the time scale is the set Z+ of positive integers and for differential equations when the time scale is the set R of real numbers.

  18. Concentration and selective fractionation of an antihypertensive peptide from an alfalfa white proteins hydrolysate by mixed ion-exchange centrifugal partition chromatography. (United States)

    Boudesocque, Leslie; Kapel, Romain; Paris, Cedric; Dhulster, Pascal; Marc, Ivan; Renault, Jean-Hugues


    This article reports a promising use of the mixed ion-exchange centrifugal partition chromatography (MIXCPC) technique in the field of downstream processes. A complex alfalfa white protein concentrate hydrolysate (AWPC hydrolysate) showing anti-hypertensive properties was successfully fractionated by MIXCPC to yield a L-valyl-L-tryptophan (VW) enriched fraction in one run. This dipeptide shows an interesting anti-angiotensin converting enzyme (anti-ACE) activity. An analytical method based on RP-LC/MS-MS was developed to quantify the target VW peptide in both the starting material and the enriched fractions. The best results for the MIXCPC fractionation were obtained by the combined use of the quaternary biphasic solvent system, methyl-tert-butylether/acetonitrile/n-butanol/water (2:1:2:5, v/v) in the descending mode, of the lipophilic di(2-ethylhexyl)phosphoric acid (DEHPA) cation-exchanger with an exchanger (DEHPA)/peptides ratio of 15, and of two displacers: calcium chloride and hydrochloric acid. The complexity of the starting material involved the selectivity optimization by splitting the stationary phase into two sections that differed by their triethylamine concentration. From 1g of AWPC hydrolysate containing 0.26% of VW, 30.7 mg of a VW enriched fraction were recovered with a purity of 10.9%, corresponding to a purification factor of 41 and a recovery of 97%.

  19. Temperature dependence of fluctuation time scales in spin glasses

    DEFF Research Database (Denmark)

    Kenning, Gregory G.; Bowen, J.; Sibani, Paolo;


    Using a series of fast cooling protocols we have probed aging effects in the spin glass state as a function of temperature. Analyzing the logarithmic decay found at very long time scales within a simple phenomenological barrier model, leads to the extraction of the fluctuation time scale of the s...

  20. Bounds of Certain Dynamic Inequalities on Time Scales

    Directory of Open Access Journals (Sweden)

    Deepak B. Pachpatte


    Full Text Available In this paper we study explicit bounds of certain dynamic integral inequalities on time scales. These estimates give the bounds on unknown functions which can be used in studying the qualitative aspects of certain dynamic equations. Using these inequalities we prove the uniqueness of some partial integro-differential equations on time scales.

  1. Virus-binding proteins recovered from bacterial culture derived from activated sludge by affinity chromatography assay using a viral capsid peptide. (United States)

    Sano, Daisuke; Matsuo, Takahiro; Omura, Tatsuo


    The contamination of water environments by pathogenic viruses has raised concerns about outbreaks of viral infectious diseases in our society. Because conventional water and wastewater treatment systems are not effective enough to inactivate or remove pathogenic viruses, a new technology for virus removal needs to be developed. In this study, the virus-binding proteins (VBPs) in a bacterial culture derived from activated sludge were successfully recovered. The recovery of VBPs was achieved by applying extracted crude proteins from a bacterial culture to an affinity column in which a custom-made peptide of capsid protein from the poliovirus type 1 (PV1) Mahoney strain (H(2)N-DNPASTTNKDKL-COOH) was immobilized as a ligand. VBPs exhibited the ability to adsorb infectious particles of PV1 Sabin 1 as determined by enzyme-linked immunosorbent assay. The evaluation of surface charges of VBPs with ion-exchange chromatography found that a majority of VBP molecules had a net negative charge under the conditions of affinity chromatography. On the other hand, a calculated isoelectric point implied that the viral peptide in the affinity column was also charged negatively. As a result, the adsorption of the VBPs to the viral peptide in the affinity column occurred with a strong attractive force that was able to overcome the electrostatic repulsive force. Two-dimensional electrophoresis revealed that the isolated VBPs include a number of proteins, and their molecular masses were widely distributed but smaller than 100 kDa. Amino acid sequences of N termini of five VBPs were determined. Homology searches for the N termini against all protein sequences in the National Center for Biotechnology Information (NCBI) database showed that the isolated VBPs in this study were newly discovered proteins. These VBPs that originated with bacteria in activated sludge might be stable, because they are existing in the environment of wastewater treatments. Therefore, a virus removal technology

  2. Solution-phase-peptide synthesis via the group-assisted purification (GAP) chemistry without using chromatography and recrystallization. (United States)

    Wu, Jianbin; An, Guanghui; Lin, Siqi; Xie, Jianbo; Zhou, Wei; Sun, Hao; Pan, Yi; Li, Guigen


    The solution phase synthesis of N-protected amino acids and peptides has been achieved through the Group-Assisted Purification (GAP) chemistry by avoiding disadvantages of other methods in regard to the difficult scale-up, expenses of solid and soluble polymers, etc. The GAP synthesis can reduce the use of solvents, silica gels, energy and manpower. In addition, the GAP auxiliary can be conveniently recovered for re-use and is environmentally friendly and benign, and substantially reduces waste production in academic labs and industry.

  3. The limit order book on different time scales

    CERN Document Server

    Eisler, Zoltan; Lillo, Fabrizio


    Financial markets can be described on several time scales. We use data from the limit order book of the London Stock Exchange (LSE) to compare how the fluctuation dominated microstructure crosses over to a more systematic global behavior.


    Institute of Scientific and Technical Information of China (English)


    In this paper we consider a nonlinear first-order boundary value problem on a time scale. The existence results of three positive solutions are obtained using fixed point theorems. Finally,examples are presented to illustrate the main results.

  5. Controllability of multiplex, multi-time-scale networks (United States)

    Pósfai, Márton; Gao, Jianxi; Cornelius, Sean P.; Barabási, Albert-László; D'Souza, Raissa M.


    The paradigm of layered networks is used to describe many real-world systems, from biological networks to social organizations and transportation systems. While recently there has been much progress in understanding the general properties of multilayer networks, our understanding of how to control such systems remains limited. One fundamental aspect that makes this endeavor challenging is that each layer can operate at a different time scale; thus, we cannot directly apply standard ideas from structural control theory of individual networks. Here we address the problem of controlling multilayer and multi-time-scale networks focusing on two-layer multiplex networks with one-to-one interlayer coupling. We investigate the practically relevant case when the control signal is applied to the nodes of one layer. We develop a theory based on disjoint path covers to determine the minimum number of inputs (Ni) necessary for full control. We show that if both layers operate on the same time scale, then the network structure of both layers equally affect controllability. In the presence of time-scale separation, controllability is enhanced if the controller interacts with the faster layer: Ni decreases as the time-scale difference increases up to a critical time-scale difference, above which Ni remains constant and is completely determined by the faster layer. We show that the critical time-scale difference is large if layer I is easy and layer II is hard to control in isolation. In contrast, control becomes increasingly difficult if the controller interacts with the layer operating on the slower time scale and increasing time-scale separation leads to increased Ni, again up to a critical value, above which Ni still depends on the structure of both layers. This critical value is largely determined by the longest path in the faster layer that does not involve cycles. By identifying the underlying mechanisms that connect time-scale difference and controllability for a simplified

  6. Detection of crossover time scales in multifractal detrended fluctuation analysis (United States)

    Ge, Erjia; Leung, Yee


    Fractal is employed in this paper as a scale-based method for the identification of the scaling behavior of time series. Many spatial and temporal processes exhibiting complex multi(mono)-scaling behaviors are fractals. One of the important concepts in fractals is crossover time scale(s) that separates distinct regimes having different fractal scaling behaviors. A common method is multifractal detrended fluctuation analysis (MF-DFA). The detection of crossover time scale(s) is, however, relatively subjective since it has been made without rigorous statistical procedures and has generally been determined by eye balling or subjective observation. Crossover time scales such determined may be spurious and problematic. It may not reflect the genuine underlying scaling behavior of a time series. The purpose of this paper is to propose a statistical procedure to model complex fractal scaling behaviors and reliably identify the crossover time scales under MF-DFA. The scaling-identification regression model, grounded on a solid statistical foundation, is first proposed to describe multi-scaling behaviors of fractals. Through the regression analysis and statistical inference, we can (1) identify the crossover time scales that cannot be detected by eye-balling observation, (2) determine the number and locations of the genuine crossover time scales, (3) give confidence intervals for the crossover time scales, and (4) establish the statistically significant regression model depicting the underlying scaling behavior of a time series. To substantive our argument, the regression model is applied to analyze the multi-scaling behaviors of avian-influenza outbreaks, water consumption, daily mean temperature, and rainfall of Hong Kong. Through the proposed model, we can have a deeper understanding of fractals in general and a statistical approach to identify multi-scaling behavior under MF-DFA in particular.

  7. Absolute quantification of Pru av 2 in sweet cherry fruit by liquid chromatography/tandem mass spectrometry with the use of a stable isotope-labelled peptide. (United States)

    Ippoushi, Katsunari; Sasanuma, Motoe; Oike, Hideaki; Kobori, Masuko; Maeda-Yamamoto, Mari


    Pru av 2, a pathogenesis-related (PR) protein present in the sweet cherry (Prunus avium L.) fruit, is the principal allergen of cherry and one of the chief causes of pollen food syndrome (oral allergy syndrome). In this study, a quantitative assay for this protein was developed with the use of the protein absolute quantification (AQUA) method, which consists of liquid chromatography/tandem mass spectrometry (LC/MS/MS) employing TGC[CAM]STDASGK[(13)C6,(15)N2], a stable isotope-labelled internal standard (SIIS) peptide. This assay gave a linear relationship (r(2)>0.99) in a concentration range (2.3-600fmol/μL), and the overall coefficient of variation (CV) for multiple tests was 14.6%. Thus, the contents of this allergenic protein in sweet cherry products could be determined using this assay. This assay should be valuable for allergological investigations of Pru av 2 in sweet cherry and detection of protein contamination in foods.

  8. Quantitative Analysis of Autoinducing Peptide I (AIP-I) from Staphylococcus aureus Cultures using Ultrahigh Performance Liquid Chromatography – High Resolving Power Mass Spectrometry (United States)

    Junio, Hiyas A.; Todd, Daniel A.; Ettefagh, Keivan A.; Ehrmann, Brandie M.; Kavanaugh, Jeffrey S.; Horswill, Alexander R.; Cech, Nadja B.


    Staphylococcus aureusinfections acquired in hospitals now cause more deaths per annum in the US than does HIV/AIDS. Perhaps even more alarming is the rise in community associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections, which have spread out of hospital settings and are infecting otherwise healthy individuals. The mechanism of enhanced pathogenesis in CA-MRSA remains unclear, but it has been postulated that high activity in the agr quorum-sensing system could be a contributing factor. The purpose of this study was to develop a quantitative method for analysis of autoinducing peptide I (AIP-I), the activating signal for the agr system in S. aureus. An effective method was developed using ultrahigh performance liquid chromatography (UHPLC) coupled to electrospray ionization mass spectrometry with an LTQ Orbitrap mass spectrometer. Relying on the exceptional resolving power and mass accuracy of this instrument configuration, it was possible to quantify AIP-I directly from the complex growth media of S. aureus cultures with a limit of detection (LOD) of 0.25 μM and a linear dynamic range of 2.3 to 63 μM. The method was then employed to monitor time-dependent production of AIP-I by S. aureus cultures, and it was observed that AIP-I production reached a maximum and leveled off after approximately 16 hrs. Finally, it was determined that virulence of S. aureus was correlated with AIP-I production in some (but not all) strains analyzed. PMID:23703543

  9. Quantitative analysis of a novel antimicrobial peptide in rat plasma by ultra performance liquid chromatography-tandem mass spectrometry

    Institute of Scientific and Technical Information of China (English)

    Ruo-Wen Zhang; Wen-Tao Liu; Lu-Lu Geng; Xiao-Hui Chen; Kai-Shun Bi


    We described the first results of a quantitative ultra performance liquid chromatographytandem mass spectrometry method for a novel antimicrobial peptide (phylloseptin, PSN-1). Chromatographic separation was accomplished on a Waters bridged ethyl hybrid (BEH) C18 (50mm× 2.1 mm, 1.7 μm) column with acetonitrile-water (25:75, v/v) as isocratic mobile phase. Mass spectrometry detection was performed in the positive electrospray ionization mode and by monitoring of the transitions at m/z 679.6/120, 509.6/120 (PSN-1) and m/z 340.7/165 (Thymopentin, IS). Protein precipitation was investigated and the recovery was satisfactory (above 82%). The method was shown to be reproducible and reliable with intra-day precision below 5.3%, inter-day precision below 14.2%, and linear range from 0.02 to 2 lag/mL with r〉0.994. The method was successfully applied to a pharmacokinetic study of PSN-1 in rats after intravenous administration.

  10. Rapid and specific high-performance liquid chromatography for the in vitro quantification of D-Lys6-GnRH in a microemulsion-type formulation in the presence of peptide oxidation products. (United States)

    Kafka, Alexandra P; Rades, Thomas; McDowell, Arlene


    A high-performance liquid chromatography (HPLC) method for assay of d-Lys(6)-GnRH contained in a microemulsion-type formulation is described. The peptide is extracted from the microemulsion matrix and quantified using a two-step gradient method. Separation from microemulsion compounds and potential peptide oxidation products was achieved on a Jupiter C(18) column at 40 degrees C, using a gradient of 10-35% CH(3)CN for peptide elution. The correlation of peak intensity measured at 220 nm and peptide concentration was linear over the range 2.5-60 microg/mL with a correlation coefficient of 0.9997 and a y-intercept not significantly different from zero (p > 0.05). Intraday and interday variability of the assay was less than 5% for multiple injections of samples containing 7.5, 30 and 60 microg/mL. The lower limit of quantitation was calculated to be 0.38 microg/mL, and the lower limit of detection was 0.13 microg/mL. The assay was applied to samples that were stressed under physiological conditions (37 degrees C, pH 7.4) over 4 days. Three degradation peaks were well resolved from the parent peptide, demonstrating the selectivity of the assay. Off-line MALDI TOF mass spectrometry was applied to identify these degradation species as oxidation products of the peptide.

  11. Characteristic Time Scales of Characteristic Magmatic Processes and Systems (United States)

    Marsh, B. D.


    Every specific magmatic process, regardless of spatial scale, has an associated characteristic time scale. Time scales associated with crystals alone are rates of growth, dissolution, settling, aggregation, annealing, and nucleation, among others. At the other extreme are the time scales associated with the dynamics of the entire magmatic system. These can be separated into two groups: those associated with system genetics (e.g., the production and transport of magma, establishment of the magmatic system) and those due to physical characteristics of the established system (e.g., wall rock failure, solidification front propagation and instability, porous flow). The detailed geometry of a specific magmatic system is particularly important to appreciate; although generic systems are useful, care must be taken to make model systems as absolutely realistic as possible. Fuzzy models produce fuzzy science. Knowledge of specific time scales is not necessarily useful or meaningful unless the hierarchical context of the time scales for a realistic magmatic system is appreciated. The age of a specific phenocryst or ensemble of phenocrysts, as determined from isotopic or CSD studies, is not meaningful unless something can be ascertained of the provenance of the crystals. For example, crystal size multiplied by growth rate gives a meaningful crystal age only if it is from a part of the system that has experienced semi-monotonic cooling prior to chilling; crystals entrained from a long-standing cumulate bed that were mechanically sorted in ascending magma may not reveal this history. Ragged old crystals rolling about in the system for untold numbers of flushing times record specious process times, telling more about the noise in the system than the life of typical, first generation crystallization processes. The most helpful process-related time scales are those that are known well and that bound or define the temporal style of the system. Perhaps the most valuable of these

  12. Time scales and species coexistence in chaotic flows

    CERN Document Server

    Galla, Tobias


    Empirical observations in marine ecosystems have suggested a balance of biological and advection time scales as a possible explanation of species coexistence. To characterise this scenario, we measure the time to fixation in neutrally evolving populations in chaotic flows. Contrary to intuition the variation of time scales does not interpolate straightforwardly between the no-flow and well-mixed limits; instead we find that fixation is the slowest at intermediate Damk\\"ohler numbers, indicating long-lasting coexistence of species. Our analysis shows that this slowdown is due to spatial organisation on an increasingly modularised network. We also find that diffusion can either slow down or speed up fixation, depending on the relative time scales of flow and evolution.

  13. Dynamics symmetries of Hamiltonian system on time scales

    Energy Technology Data Exchange (ETDEWEB)

    Peng, Keke, E-mail:; Luo, Yiping, E-mail: [Department of Physics, Zhejiang Sci-Tech University, Hangzhou 310018 (China)


    In this paper, the dynamics symmetries of Hamiltonian system on time scales are studied. We study the symmetries and quantities based on the calculation of variation and Lie transformation group. Particular focus lies in: the Noether symmetry leads to the Noether conserved quantity and the Lie symmetry leads to the Noether conserved quantity if the infinitesimal transformations satisfy the structure equation. As the new application of result, at end of the article, we give a simple example of Noether symmetry and Lie symmetry on time scales.

  14. Multiple time scales of fluvial processes—theory and applications

    Institute of Scientific and Technical Information of China (English)


    Fluvial processes comprise water flow,sediment transport and bed evolution,which normally feature distinct time scales.The time scales of sediment transport and bed deformation relative to the flow essentially measure how fast sediment transport adapts to capacity region in line with local flow scenario and the bed deforms in comparison with the flow,which literally dictates if a capacity based and/or decoupled model is justified.This paper synthesizes the recently developed multiscale theory for sediment-l...

  15. Dynamics symmetries of Hamiltonian system on time scales (United States)

    Peng, Keke; Luo, Yiping


    In this paper, the dynamics symmetries of Hamiltonian system on time scales are studied. We study the symmetries and quantities based on the calculation of variation and Lie transformation group. Particular focus lies in: the Noether symmetry leads to the Noether conserved quantity and the Lie symmetry leads to the Noether conserved quantity if the infinitesimal transformations satisfy the structure equation. As the new application of result, at end of the article, we give a simple example of Noether symmetry and Lie symmetry on time scales.

  16. Short—Time Scaling of Variable Ordering of OBDDs

    Institute of Scientific and Technical Information of China (English)

    龙望宁; 闵应骅; 等


    A short-time scaling criterion of variable ordering of OBDDs is proposed.By this criterion it is easy and fast to determine which one is better when several variable orders are given,especially when they differ 10% or more in resulted BDD size from each other.An adaptive variable order selection method,based on the short-time scaling criterion,is also presented.The experimental results show that this method is efficient and it makes the heuristic variable ordering methods more practical.

  17. Time scale bias in erosion rates of glaciated landscapes. (United States)

    Ganti, Vamsi; von Hagke, Christoph; Scherler, Dirk; Lamb, Michael P; Fischer, Woodward W; Avouac, Jean-Philippe


    Deciphering erosion rates over geologic time is fundamental for understanding the interplay between climate, tectonic, and erosional processes. Existing techniques integrate erosion over different time scales, and direct comparison of such rates is routinely done in earth science. On the basis of a global compilation, we show that erosion rate estimates in glaciated landscapes may be affected by a systematic averaging bias that produces higher estimated erosion rates toward the present, which do not reflect straightforward changes in erosion rates through time. This trend can result from a heavy-tailed distribution of erosional hiatuses (that is, time periods where no or relatively slow erosion occurs). We argue that such a distribution can result from the intermittency of erosional processes in glaciated landscapes that are tightly coupled to climate variability from decadal to millennial time scales. In contrast, we find no evidence for a time scale bias in spatially averaged erosion rates of landscapes dominated by river incision. We discuss the implications of our findings in the context of the proposed coupling between climate and tectonics, and interpreting erosion rate estimates with different averaging time scales through geologic time.

  18. Speech Compensation for Time-Scale-Modified Auditory Feedback (United States)

    Ogane, Rintaro; Honda, Masaaki


    Purpose: The purpose of this study was to examine speech compensation in response to time-scale-modified auditory feedback during the transition of the semivowel for a target utterance of /ija/. Method: Each utterance session consisted of 10 control trials in the normal feedback condition followed by 20 perturbed trials in the modified auditory…

  19. Wind power impacts and electricity storage - a time scale perspective

    DEFF Research Database (Denmark)

    Hedegaard, Karsten; Meibom, Peter


    technologies – batteries, flow batteries, compressed air energy storage, electrolysis combined with fuel cells, and electric vehicles – are moreover categorised with respect to the time scales at which they are suited to support wind power integration. While all of these technologies are assessed suitable...

  20. Modelling of UV radiation variations at different time scales

    Directory of Open Access Journals (Sweden)

    J. L. Borkowski


    Full Text Available Solar UV radiation variability in the period 1976–2006 is discussed with respect to the relative changes in the solar global radiation, ozone content, and cloudiness. All the variables were decomposed into separate components, representing variations of different time scales, using wavelet multi-resolution decomposition. The response of the UV radiation to the changes in the solar global radiation, ozone content, and cloudiness depends on the time scale, therefore, it seems reasonable to model separately the relation between UV and explanatory variables at different time scales. The wavelet components of the UV series are modelled and summed to obtain the fit of observed series. The results show that the coarser time scale components can be modelled with greater accuracy than fine scale components and the fitted values calculated by this method are in better agreement with observed values than values calculated by the regression method, in which variables were not decomposed. The residual standard error in the case of modelling with the use of wavelets is reduced by 14% in comparison to the regression method without decomposition.

  1. Quadratic Lyapunov Function and Exponential Dichotomy on Time Scales

    Institute of Scientific and Technical Information of China (English)



    In this paper, we study the relationship between exponential dichotomy and quadratic Lyapunov function for the linear equation x△ = A(t)x on time scales.Moreover, for the nonlinear perturbed equation x△ = A(t)x + f(t,x) we give the instability of the zero solution when f is sufficiently small.

  2. Exponential stability of dynamic equations on time scales

    Directory of Open Access Journals (Sweden)

    Raffoul Youssef N


    Full Text Available We investigate the exponential stability of the zero solution to a system of dynamic equations on time scales. We do this by defining appropriate Lyapunov-type functions and then formulate certain inequalities on these functions. Several examples are given.

  3. Obesity-Related Metabolomic Analysis of Human Subjects in Black Soybean Peptide Intervention Study by Ultraperformance Liquid Chromatography and Quadrupole-Time-of-Flight Mass Spectrometry

    Directory of Open Access Journals (Sweden)

    Min Jung Kim


    Full Text Available The present study aimed to identify key metabolites related to weight reduction in humans by studying the metabolic profiles of sera obtained from 34 participants who underwent dietary intervention with black soybean peptides (BSP for 12 weeks. This research is a sequel to our previous work in which the effects of BSP on BMI and blood composition of lipid were investigated. Sera of the study were subjected to ultra performance liquid chromatography and quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS, and the data were analyzed using partial least-squares discriminate analysis (PLS-DA score plots. Body mass index and percent body fat of the test group were reduced. Levels of betaine, benzoic acid, pyroglutamic acid, pipecolic acid, N-phenylacetamide, uric acid, l-aspartyl-l-phenylalanine, and lysophosphatidyl cholines (lysoPCs (C18:1, C18:2, C20:1, and C20:4 showed significant increases. Levels of l-proline, valine, l-leucine/isoleucine, hypoxanthine, glutamine, l-methionine, phenylpyruvic acid, several carnitine derivatives, and lysoPCs (C14:0, PC16:0, C15:0, C16:0, C17:1, C18:0, and C22:0 were significantly decreased. In particular, lysoPC 16:0 with a VIP value of 12.02 is esteemed to be the most important metabolite for evaluating the differences between the 2 serum samples. Our result confirmed weight-lowering effects of BSP, accompanied by favorable changes in metabolites in the subjects’ blood. Therefore, this research enables us to better understand obesity and increases the predictability of the obesity-related risk by studying metabolites present in the blood.

  4. Simultaneous Glycan-Peptide Characterization Using Hydrophilic Interaction Chromatography and Parallel Fragmentation by CID, Higher Energy Collisional Dissociation, and Electron Transfer Dissociation MS Applied to the N-Linked Glycoproteome of Campylobacter jejuni* (United States)

    Scott, Nichollas E.; Parker, Benjamin L.; Connolly, Angela M.; Paulech, Jana; Edwards, Alistair V. G.; Crossett, Ben; Falconer, Linda; Kolarich, Daniel; Djordjevic, Steven P.; Højrup, Peter; Packer, Nicolle H.; Larsen, Martin R.; Cordwell, Stuart J.


    Campylobacter jejuni is a gastrointestinal pathogen that is able to modify membrane and periplasmic proteins by the N-linked addition of a 7-residue glycan at the strict attachment motif (D/E)XNX(S/T). Strategies for a comprehensive analysis of the targets of glycosylation, however, are hampered by the resistance of the glycan-peptide bond to enzymatic digestion or β-elimination and have previously concentrated on soluble glycoproteins compatible with lectin affinity and gel-based approaches. We developed strategies for enriching C. jejuni HB93-13 glycopeptides using zwitterionic hydrophilic interaction chromatography and examined novel fragmentation, including collision-induced dissociation (CID) and higher energy collisional (C-trap) dissociation (HCD) as well as CID/electron transfer dissociation (ETD) mass spectrometry. CID/HCD enabled the identification of glycan structure and peptide backbone, allowing glycopeptide identification, whereas CID/ETD enabled the elucidation of glycosylation sites by maintaining the glycan-peptide linkage. A total of 130 glycopeptides, representing 75 glycosylation sites, were identified from LC-MS/MS using zwitterionic hydrophilic interaction chromatography coupled to CID/HCD and CID/ETD. CID/HCD provided the majority of the identifications (73 sites) compared with ETD (26 sites). We also examined soluble glycoproteins by soybean agglutinin affinity and two-dimensional electrophoresis and identified a further six glycosylation sites. This study more than doubles the number of confirmed N-linked glycosylation sites in C. jejuni and is the first to utilize HCD fragmentation for glycopeptide identification with intact glycan. We also show that hydrophobic integral membrane proteins are significant targets of glycosylation in this organism. Our data demonstrate that peptide-centric approaches coupled to novel mass spectrometric fragmentation techniques may be suitable for application to eukaryotic glycoproteins for simultaneous

  5. Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the N-linked glycoproteome of Campylobacter jejuni. (United States)

    Scott, Nichollas E; Parker, Benjamin L; Connolly, Angela M; Paulech, Jana; Edwards, Alistair V G; Crossett, Ben; Falconer, Linda; Kolarich, Daniel; Djordjevic, Steven P; Højrup, Peter; Packer, Nicolle H; Larsen, Martin R; Cordwell, Stuart J


    Campylobacter jejuni is a gastrointestinal pathogen that is able to modify membrane and periplasmic proteins by the N-linked addition of a 7-residue glycan at the strict attachment motif (D/E)XNX(S/T). Strategies for a comprehensive analysis of the targets of glycosylation, however, are hampered by the resistance of the glycan-peptide bond to enzymatic digestion or β-elimination and have previously concentrated on soluble glycoproteins compatible with lectin affinity and gel-based approaches. We developed strategies for enriching C. jejuni HB93-13 glycopeptides using zwitterionic hydrophilic interaction chromatography and examined novel fragmentation, including collision-induced dissociation (CID) and higher energy collisional (C-trap) dissociation (HCD) as well as CID/electron transfer dissociation (ETD) mass spectrometry. CID/HCD enabled the identification of glycan structure and peptide backbone, allowing glycopeptide identification, whereas CID/ETD enabled the elucidation of glycosylation sites by maintaining the glycan-peptide linkage. A total of 130 glycopeptides, representing 75 glycosylation sites, were identified from LC-MS/MS using zwitterionic hydrophilic interaction chromatography coupled to CID/HCD and CID/ETD. CID/HCD provided the majority of the identifications (73 sites) compared with ETD (26 sites). We also examined soluble glycoproteins by soybean agglutinin affinity and two-dimensional electrophoresis and identified a further six glycosylation sites. This study more than doubles the number of confirmed N-linked glycosylation sites in C. jejuni and is the first to utilize HCD fragmentation for glycopeptide identification with intact glycan. We also show that hydrophobic integral membrane proteins are significant targets of glycosylation in this organism. Our data demonstrate that peptide-centric approaches coupled to novel mass spectrometric fragmentation techniques may be suitable for application to eukaryotic glycoproteins for simultaneous

  6. Oligocene calibration of the magnetic polarity time scale (United States)

    Prothero, Donald R.; Denham, Charles R.; Farmer, Harlow G.


    Magnetostratigraphic studies of the Oligocene White River Group in Wyoming, Colorado, Nebraska, and the Dakotas yield a radiometrically dated polarity stratigraphy that provides mid-Tertiary calibration points for the magnetic polarity time scale. Anomaly 12 13 reversal is bracketed by dates of 32.4 and 34.6 m.y., in best agreement with the time scale of LaBrecque and colleagues. The magnetostratigraphy also helps calibrate the Oligocene North American land mammal “ages” and allows correlation with the European marine microfossil zonation. This correlation suggests that the age of the Eocene-Oligocene boundary is 37.0 m.y., contrary to younger dates obtained from glauconites and microtektites. *Present address: Department of Geology, Knox College, Galesburg, Illinois 61401

  7. Long-term variation time scales in OJ 287

    Institute of Scientific and Technical Information of China (English)

    Jun-Hui Fan; Yi Liu; Bo-Chun Qian; Jun Tao; Zhi-Qiang Shen; Jiang-Shui Zhang; Yong Huang; Jin Wang


    The light curve data from 1894 to 2008 are compiled for the BL Lacertae object OJ 287 from the available literature. Periodicity analysis methods (the Discrete Correlation Function-DCE the Jurkevich method, the power spectral (Fourier) analysis, and the CLEANest method) are performed to search for possible periodicites in the light curve of OJ 287. Significance levels are given for the possible periods. The analysis results confirm the existence of the 12.2 ± 0.6 yr time scale and show a hint of a~53 yr time scale. The 12.2 ± 0.6 yr period is used as the orbital period to investigate the supermassive binary black hole system parameters.

  8. HMC algorithm with multiple time scale integration and mass preconditioning

    CERN Document Server

    Urbach, C; Shindler, A; Wenger, U


    We present a variant of the HMC algorithm with mass preconditioning (Hasenbusch acceleration) and multiple time scale integration. We have tested this variant for standard Wilson fermions at beta=5.6 and at pion masses ranging from 380 MeV to 680 MeV. We show that in this situation its performance is comparable to the recently proposed HMC variant with domain decomposition as preconditioner. We give an update of the ``Berlin Wall'' figure, comparing the performance of our variant of the HMC algorithm to other published performance data. Advantages of the HMC algorithm with mass preconditioning and multiple time scale integration are that it is straightforward to implement and can be used in combination with a wide variety of lattice Dirac operators.

  9. HMC algorithm with multiple time scale integration and mass preconditioning (United States)

    Urbach, C.; Jansen, K.; Shindler, A.; Wenger, U.


    We present a variant of the HMC algorithm with mass preconditioning (Hasenbusch acceleration) and multiple time scale integration. We have tested this variant for standard Wilson fermions at β=5.6 and at pion masses ranging from 380 to 680 MeV. We show that in this situation its performance is comparable to the recently proposed HMC variant with domain decomposition as preconditioner. We give an update of the "Berlin Wall" figure, comparing the performance of our variant of the HMC algorithm to other published performance data. Advantages of the HMC algorithm with mass preconditioning and multiple time scale integration are that it is straightforward to implement and can be used in combination with a wide variety of lattice Dirac operators.

  10. Cognitive componets of speech at different time scales

    DEFF Research Database (Denmark)

    Feng, Ling; Hansen, Lars Kai


    Cognitive component analysis (COCA) is defined as unsupervised grouping of data leading to a group structure well aligned with that resulting from human cognitive activity. We focus here on speech at different time scales looking for possible hidden ‘cognitive structure’. Statistical regularities......, assumed to model the basic representation of the human auditory system. The basic features are aggregated in time to obtain features at longer time scales. Simple energy based filtering is used to achieve a sparse representation. Our hypothesis is now basically ecological: We hypothesize that features...... that are essentially independent in a reasonable ensemble can be efficiently coded using a sparse independent component representation. The representations are indeed shown to be very similar between supervised learning (invoking cognitive activity) and unsupervised learning (statistical regularities), hence lending...

  11. Time Scales and Tidal Effects in Minor Mergers

    Institute of Scientific and Technical Information of China (English)

    Yu Lu; Jian-Yan Wei


    We use controlled N-body simulation to investigate the dynamical processes (dynamical friction, tidal truncation, etc.) involved in the merging of small satellites into bigger halos. We confirm the validity of some analytic formulae proposed earlier based on simple arguments. For rigid satellites represented by softened point masses, the merging time scale depends on both the orbital shape and concentration of the satellite. The dependence on orbital ellipticity is roughly a power law, as suggested by Lacey & Cole, and the dependence on satellite concentration is similar to that proposed by White. When merging satellites are represented by non-rigid objects, Tidal effects must be considered. We found that material beyond the tidal radius are stripped off. The decrease in the satellite mass might mean an increase in the merging time scale, but in fact, the merging time is decreased,because the stripped-off material carries away a proportionately larger amount of of orbital energy and angular momentum.

  12. Human learning: Power laws or multiple characteristic time scales?

    Directory of Open Access Journals (Sweden)

    Gottfried Mayer-Kress


    Full Text Available The central proposal of A. Newell and Rosenbloom (1981 was that the power law is the ubiquitous law of learning. This proposition is discussed in the context of the key factors that led to the acceptance of the power law as the function of learning. We then outline the principles of an epigenetic landscape framework for considering the role of the characteristic time scales of learning and an approach to system identification of the processes of performance dynamics. In this view, the change of performance over time is the product of a superposition of characteristic exponential time scales that reflect the influence of different processes. This theoretical approach can reproduce the traditional power law of practice – within the experimental resolution of performance data sets - but we hypothesize that this function may prove to be a special and perhaps idealized case of learning.

  13. Realization of a time-scale with an optical clock

    CERN Document Server

    Grebing, C; Dörscher, S; Häfner, S; Gerginov, V; Weyers, S; Lipphardt, B; Riehle, F; Sterr, U; Lisdat, C


    Optical clocks are not only powerful tools for prime fundamental research, but are also deemed for the re-definition of the SI base unit second as they surpass the performance of caesium atomic clocks in both accuracy and stability by more than an order of magnitude. However, an important obstacle in this transition has so far been the limited reliability of the optical clocks that made a continuous realization of a time-scale impractical. In this paper, we demonstrate how this dilemma can be resolved and that a time-scale based on an optical clock can be established that is superior to one based on even the best caesium fountain clocks. The paper also gives further proof of the international consistency of strontium lattice clocks on the $10^{-16}$ accuracy level, which is another prerequisite for a change in the definition of the second.

  14. Time scales of crystal mixing in magma mushes (United States)

    Schleicher, Jillian M.; Bergantz, George W.; Breidenthal, Robert E.; Burgisser, Alain


    Magma mixing is widely recognized as a means of producing compositional diversity and preconditioning magmas for eruption. However, the processes and associated time scales that produce the commonly observed expressions of magma mixing are poorly understood, especially under crystal-rich conditions. Here we introduce and exemplify a parameterized method to predict the characteristic mixing time of crystals in a crystal-rich magma mush that is subject to open-system reintrusion events. Our approach includes novel numerical simulations that resolve multiphase particle-fluid interactions. It also quantifies the crystal mixing by calculating both the local and system-wide progressive loss of the spatial correlation of individual crystals throughout the mixing region. Both inertial and viscous time scales for bulk mixing are introduced. Estimated mixing times are compared to natural examples and the time for basaltic mush systems to become well mixed can be on the order of 10 days.

  15. Exponentials and Laplace transforms on nonuniform time scales (United States)

    Ortigueira, Manuel D.; Torres, Delfim F. M.; Trujillo, Juan J.


    We formulate a coherent approach to signals and systems theory on time scales. The two derivatives from the time-scale calculus are used, i.e., nabla (forward) and delta (backward), and the corresponding eigenfunctions, the so-called nabla and delta exponentials, computed. With these exponentials, two generalised discrete-time Laplace transforms are deduced and their properties studied. These transforms are compatible with the standard Laplace and Z transforms. They are used to study discrete-time linear systems defined by difference equations. These equations mimic the usual continuous-time equations that are uniformly approximated when the sampling interval becomes small. Impulse response and transfer function notions are introduced. This implies a unified mathematical framework that allows us to approximate the classic continuous-time case when the sampling rate is high or to obtain the standard discrete-time case, based on difference equations, when the time grid becomes uniform.

  16. Time scales for molecule formation by ion-molecule reactions (United States)

    Langer, W. D.; Glassgold, A. E.


    Analytical solutions are obtained for nonlinear differential equations governing the time-dependence of molecular abundances in interstellar clouds. Three gas-phase reaction schemes are considered separately for the regions where each dominates. The particular case of CO, and closely related members of the Oh and CH families of molecules, is studied for given values of temperature, density, and the radiation field. Nonlinear effects and couplings with particular ions are found to be important. The time scales for CO formation range from 100,000 to a few million years, depending on the chemistry and regime. The time required for essentially complete conversion of C(+) to CO in the region where the H3(+) chemistry dominates is several million years. Because this time is longer than or comparable to dynamical time scales for dense interstellar clouds, steady-state abundances may not be observed in such clouds.

  17. Entropy Production of Nanosystems with Time Scale Separation (United States)

    Wang, Shou-Wen; Kawaguchi, Kyogo; Sasa, Shin-ichi; Tang, Lei-Han


    Energy flows in biomolecular motors and machines are vital to their function. Yet experimental observations are often limited to a small subset of variables that participate in energy transport and dissipation. Here we show, through a solvable Langevin model, that the seemingly hidden entropy production is measurable through the violation spectrum of the fluctuation-response relation of a slow observable. For general Markov systems with time scale separation, we prove that the violation spectrum exhibits a characteristic plateau in the intermediate frequency region. Despite its vanishing height, the plateau can account for energy dissipation over a broad time scale. Our findings suggest a general possibility to probe hidden entropy production in nanosystems without direct observation of fast variables.

  18. Inferring Patterns in Network Traffic: Time Scales and Variations (United States)


    2014 Carnegie Mellon University Inferring Patterns in Network Traffic : Time Scales and Variation Soumyo Moitra 1. REPORT DATE 21 OCT 2014 2. REPORT TYPE N/A 3. DATES COVERED 4. TITLE AND SUBTITLE Inferring Patterns in Network Traffic : Time...method and metrics for Situational Awareness • SA  Monitoring trends and changes in traffic • Analysis over time  Time series data analysis • Metrics

  19. Isoperimetric problems on time scales with nabla derivatives


    Almeida, Ricardo; Torres, Delfim F. M.


    We prove a necessary optimality condition for isoperimetric problems under nabla-differentiable curves. As a consequence, the recent results of [M.R. Caputo, A unified view of ostensibly disparate isoperimetric variational problems, Appl. Math. Lett. (2008), doi:10.1016/j.aml.2008.04.004], that put together seemingly dissimilar optimal control problems in economics and physics, are extended to a generic time scale. We end with an illustrative example of application of our main result to a dyn...


    Institute of Scientific and Technical Information of China (English)

    Jean Louis Woukeng; David Dongo


    We study the multiscale homogenization of a nonlinear hyperbolic equation in a periodic setting. We obtain an accurate homogenization result. We also show that as the nonlinear term depends on the microscopic time variable, the global homogenized problem thus obtained is a system consisting of two hyperbolic equations. It is also shown that in spite of the presence of several time scales, the global homogenized problem is not a reiterated one.

  1. The fission time scale measured with an atomic clock

    NARCIS (Netherlands)

    Kravchuk, VL; Wilschut, HW; Hunyadi, M; Kopecky, S; Lohner, H; Rogachevskiy, A; Siemssen, RH; Krasznahorkay, A; Hamilton, JH; Ramayya, AV; Carter, HK


    We present a new direct method of measuring the fission absolute time scale using an atomic clock based on the lifetime of a vacancy in the atomic K-shell. We studied the reaction Ne-20 + Th-232 -> O-16 + U-236* at 30 MeV/u. The excitation energy of about 115 MeV in such a reaction is in the range w

  2. Midfrontal theta tracks action monitoring over multiple interactive time scales. (United States)

    Cohen, Michael X


    Quickly detecting and correcting mistakes is a crucial brain function. EEG studies have identified an idiosyncratic electrophysiological signature of online error correction, termed midfrontal theta. Midfrontal theta has so far been investigated over the fast time-scale of a few hundred milliseconds. But several aspects of behavior and brain activity unfold over multiple time scales, displaying "scale-free" dynamics that have been linked to criticality and optimal flexibility when responding to changing environmental demands. Here we used a novel line-tracking task to demonstrate that midfrontal theta is a transient yet non-phase-locked response that is modulated by task performance over at least three time scales: a few hundred milliseconds at the onset of a mistake, task performance over a fixed window of the previous 5s, and scale-free-like fluctuations over many tens of seconds. These findings provide novel evidence for a role of midfrontal theta in online behavioral adaptation, and suggest new approaches for linking EEG signatures of human executive functioning to its neurobiological underpinnings.

  3. A stable Cenozoic geologic time scale is indispensable

    Institute of Scientific and Technical Information of China (English)

    Amos Salvador


    @@ A stable, standard geologic time scale is indispensable for the clear and precise communication among geologists; it is a basic tool of geologic work. Considerable progress has been made to achieve such a stable time scale. However, during the last few years several proposals have been made to modify the Cenozoic section of the geologic time scale that threaten to destabilize it.Seven articles published in Episodes since 2000 that could contribute to this destabilization are discussed.They provide excellent examples of the profusion of different terminologies, hierarchies, and stratigraphic relationships that have been proposed: to eliminate the Tertiary and the Quaternary or to raise their rank to suberathems; to extend the Neogene to the present; to make the Quaternary a formal subsystem of the Neogene, or consider it an informal stratigraphic unit; to eliminate the Holocene, and to decouple the base of the Pleistocene from the base of the Quaternary. If adopted,these proposals would cause nothing but great confusion and controversy. They disregard the clear preferences of geologists the world over as reflected by the terminology they have been using for many decades. Common sense would dictate the continued use of this terminology in its current, widely accepted form.

  4. Diagonal chromatography to study plant protein modifications

    NARCIS (Netherlands)

    Walton, Alan; Tsiatsiani, Liana; Jacques, Silke; Stes, Elisabeth; Messens, Joris; Van Breusegem, Frank; Goormachtig, Sofie; Gevaert, Kris


    An interesting asset of diagonal chromatography, which we have introduced for contemporary proteome research, is its high versatility concerning proteomic applications. Indeed, the peptide modification or sorting step that is required between consecutive peptide separations can easily be altered and

  5. Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, HCD and ETD-MS applied to the N-linked glycoproteome of Campylobacter jejuni

    DEFF Research Database (Denmark)

    Scott, Nichollas E; Parker, Benjamin L; Connolly, Angela M;


    Campylobacter jejuni is a gastrointestinal pathogen that is able to modify membrane and periplasmic proteins by the N-linked addition of a 7-residue glycan at the strict attachment motif, D/E-X-N-X-S/T. Strategies for a comprehensive analysis of the targets of glycosylation are, however, hampered...... chromatography (ZIC-HILIC) and examined novel fragmentation, including collision-induced dissociation (CID) and higher energy collisional (C-trap) dissociation (HCD), as well as CID / electron transfer dissociation (ETD) mass spectrometry. CID/HCD enabled the identification of glycan structure and peptide......-linked glycosylation sites in C. jejuni and is the first to utilize HCD fragmentation for glycopeptide identification with intact glycan. We also show that hydrophobic integral membrane proteins are significant targets of glycosylation in this organism. Our data demonstrate that peptide-centric approaches coupled...

  6. The Role of Time-Scales in Socio-hydrology (United States)

    Blöschl, Günter; Sivapalan, Murugesu


    Much of the interest in hydrological modeling in the past decades revolved around resolving spatial variability. With the rapid changes brought about by human impacts on the hydrologic cycle, there is now an increasing need to refocus on time dependency. We present a co-evolutionary view of hydrologic systems, in which every part of the system including human systems, co-evolve, albeit at different rates. The resulting coupled human-nature system is framed as a dynamical system, characterized by interactions of fast and slow time scales and feedbacks between environmental and social processes. This gives rise to emergent phenomena such as the levee effect, adaptation to change and system collapse due to resource depletion. Changing human values play a key role in the emergence of these phenomena and should therefore be considered as internal to the system in a dynamic way. The co-evolutionary approach differs from the traditional view of water resource systems analysis as it allows for path dependence, multiple equilibria, lock-in situations and emergent phenomena. The approach may assist strategic water management for long time scales through facilitating stakeholder participation, exploring the possibility space of alternative futures, and helping to synthesise the observed dynamics of different case studies. Future research opportunities include the study of how changes in human values are connected to human-water interactions, historical analyses of trajectories of system co-evolution in individual places and comparative analyses of contrasting human-water systems in different climate and socio-economic settings. Reference Sivapalan, M. and G. Blöschl (2015) Time scale interactions and the coevolution of humans and water. Water Resour. Res., 51, 6988-7022, doi:10.1002/2015WR017896.

  7. Multiple time scale behaviors and network dynamics in liquid methanol. (United States)

    Sharma, Ruchi; Chakravarty, Charusita; Milotti, Edoardo


    Canonical ensemble molecular dynamics simulations of liquid methanol, modeled using a rigid-body, pair-additive potential, are used to compute static distributions and temporal correlations of tagged molecule potential energies as a means of characterizing the liquid state dynamics. The static distribution of tagged molecule potential energies shows a clear multimodal structure with three distinct peaks, similar to those observed previously in water and liquid silica. The multimodality is shown to originate from electrostatic effects, but not from local, hydrogen bond interactions. An interesting outcome of this study is the remarkable similarity in the tagged potential energy power spectra of methanol, water, and silica, despite the differences in the underlying interactions and the dimensionality of the network. All three liquids show a distinct multiple time scale (MTS) regime with a 1/ f (alpha) dependence with a clear positive correlation between the scaling exponent alpha and the diffusivity. The low-frequency limit of the MTS regime is determined by the frequency of crossover to white noise behavior which occurs at approximately 0.1 cm (-1) in the case of methanol under standard temperature and pressure conditions. The power spectral regime above 200 cm (-1) in all three systems is dominated by resonances due to localized vibrations, such as librations. The correlation between alpha and the diffusivity in all three liquids appears to be related to the strength of the coupling between the localized motions and the larger length/time scale network reorganizations. Thus, the time scales associated with network reorganization dynamics appear to be qualitatively similar in these systems, despite the fact that water and silica both display diffusional anomalies but methanol does not.

  8. Tunneling time scale of under-the-barrier forerunners

    CERN Document Server

    García-Calderón, G; Garcia-Calderon, Gaston; Villavicencio, Jorge


    Time-dependent analytical solutions to Schr\\"{o}dinger's equation with quantum shutter initial conditions are used to investigate the issue of the tunneling time of forerunners in rectangular potential barriers. By using a time-frequency analysis, we find the existence of a regime characterized by the opacity of the barrier, where the maximum peak of a forerunner in time domain corresponds to a genuine tunneling process. The corresponding time scale represents the tunneling time of the forerunner through the classically forbidden region.

  9. Anomalous multiphoton photoelectric effect in ultrashort time scales. (United States)

    Kupersztych, J; Raynaud, M


    In a multiphoton photoelectric process, an electron needs to absorb a given number of photons to escape the surface of a metal. It is shown for the first time that this number is not a constant depending only on the characteristics of the metal and light, but varies with the interaction duration in ultrashort time scales. The phenomenon occurs when electromagnetic energy is transferred, via ultrafast excitation of electron collective modes, to conduction electrons in a duration less than the electron energy damping time. It manifests itself through a dramatic increase of electron production.

  10. Evidence for two time scales in long SNS junctions. (United States)

    Chiodi, F; Aprili, M; Reulet, B


    We use microwave excitation to elucidate the dynamics of long superconductor-normal metal-superconductor Josephson junctions. By varying the excitation frequency in the range 10 MHz-40 GHz, we observe that the critical and retrapping currents, deduced from the dc voltage versus dc current characteristics of the junction, are set by two different time scales. The critical current increases when the ac frequency is larger than the inverse diffusion time in the normal metal, whereas the retrapping current is strongly modified when the excitation frequency is above the electron-phonon rate in the normal metal. Therefore the critical and retrapping currents are associated with elastic and inelastic scattering, respectively.

  11. Formation processes and time scales for meteorite parent bodies (United States)

    Weidenschilling, S. J.


    The transition from small particles suspended in the solar nebula to the planetesimals (asteroids) that became the parent bodies of meteorites is examined. Planetesimals probably grew by coagulation of grain aggregates that collided due to different rates of settling and drag-induced orbital decay. Their growth was accompanied by radial transport of solids, possibly sufficient to deplete the primordial mass in the asteroid zone, but with relatively little mixing. The formation of asteroid-sized planetesimals was probably rapid, on a time scale less than 1 Myr.

  12. Complex processes from dynamical architectures with time-scale hierarchy.

    Directory of Open Access Journals (Sweden)

    Dionysios Perdikis

    Full Text Available The idea that complex motor, perceptual, and cognitive behaviors are composed of smaller units, which are somehow brought into a meaningful relation, permeates the biological and life sciences. However, no principled framework defining the constituent elementary processes has been developed to this date. Consequently, functional configurations (or architectures relating elementary processes and external influences are mostly piecemeal formulations suitable to particular instances only. Here, we develop a general dynamical framework for distinct functional architectures characterized by the time-scale separation of their constituents and evaluate their efficiency. Thereto, we build on the (phase flow of a system, which prescribes the temporal evolution of its state variables. The phase flow topology allows for the unambiguous classification of qualitatively distinct processes, which we consider to represent the functional units or modes within the dynamical architecture. Using the example of a composite movement we illustrate how different architectures can be characterized by their degree of time scale separation between the internal elements of the architecture (i.e. the functional modes and external interventions. We reveal a tradeoff of the interactions between internal and external influences, which offers a theoretical justification for the efficient composition of complex processes out of non-trivial elementary processes or functional modes.

  13. Complex processes from dynamical architectures with time-scale hierarchy. (United States)

    Perdikis, Dionysios; Huys, Raoul; Jirsa, Viktor


    The idea that complex motor, perceptual, and cognitive behaviors are composed of smaller units, which are somehow brought into a meaningful relation, permeates the biological and life sciences. However, no principled framework defining the constituent elementary processes has been developed to this date. Consequently, functional configurations (or architectures) relating elementary processes and external influences are mostly piecemeal formulations suitable to particular instances only. Here, we develop a general dynamical framework for distinct functional architectures characterized by the time-scale separation of their constituents and evaluate their efficiency. Thereto, we build on the (phase) flow of a system, which prescribes the temporal evolution of its state variables. The phase flow topology allows for the unambiguous classification of qualitatively distinct processes, which we consider to represent the functional units or modes within the dynamical architecture. Using the example of a composite movement we illustrate how different architectures can be characterized by their degree of time scale separation between the internal elements of the architecture (i.e. the functional modes) and external interventions. We reveal a tradeoff of the interactions between internal and external influences, which offers a theoretical justification for the efficient composition of complex processes out of non-trivial elementary processes or functional modes.

  14. Quantification of glycated N-terminal peptide of hemoglobin using derivatization for multiple functional groups of amino acids followed by liquid chromatography/tandem mass spectrometry. (United States)

    Sakaguchi, Yohei; Kinumi, Tomoya; Yamazaki, Taichi; Takatsu, Akiko


    A novel method of amino acid analysis using derivatization of multiple functional groups (amino, carboxyl, and phenolic hydroxyl groups) was applied to measure glycated amino acids in order to quantify glycated peptides and evaluate the degree of glycation of peptide. Amino and carboxyl groups of amino acids were derivatized with 1-bromobutane so that the hydrophobicities and basicities of the amino acids, including glycated amino acids, were improved. These derivatized amino acids could be detected with high sensitivity using LC-MS/MS. In this study, 1-deoxyfructosyl-VHLTPE and VHLTPE, which are N-terminal peptides of the β-chains of hemoglobin, were selected as target compounds. After reducing the peptide sample solution with sodium borohydride, the obtained peptides were hydrolyzed with hydrochloric acid. The released amino acids were then derivatized with 1-bromobutane and analyzed with LC-MS/MS. The derivatized amino acids, including glycated amino acids, could be separated using an octadecyl silylated silica column and good sharp peaks were detected. We show a confirmatory experiment that the proposed method can be applied to evaluate the degree of glycation of peptides, using mixtures of glycated and non-glycated peptide.

  15. Stability theory for dynamic equations on time scales

    CERN Document Server

    Martynyuk, Anatoly A


    This monograph is a first in the world to present three approaches for stability analysis of solutions of dynamic equations. The first approach is based on the application of dynamic integral inequalities and the fundamental matrix of solutions of linear approximation of dynamic equations. The second is based on the generalization of the direct Lyapunovs method for equations on time scales, using scalar, vector and matrix-valued auxiliary functions. The third approach is the application of auxiliary functions (scalar, vector, or matrix-valued ones) in combination with differential dynamic inequalities. This is an alternative comparison method, developed for time continuous and time discrete systems. In recent decades, automatic control theory in the study of air- and spacecraft dynamics and in other areas of modern applied mathematics has encountered problems in the analysis of the behavior of solutions of time continuous-discrete linear and/or nonlinear equations of perturbed motion. In the book “Men of Ma...

  16. Nonoscillation for second order sublinear dynamic equations on time scales (United States)

    Erbe, Lynn; Baoguo, Jia; Peterson, Allan


    Consider the Emden-Fowler sublinear dynamic equation x[Delta][Delta](t)+p(t)f(x([sigma](t)))=0, where , is a time scale, , where ai>0, 0researchers. In this paper, we allow the coefficient function p(t) to be negative for arbitrarily large values of t. We extend a nonoscillation result of Wong for the second order sublinear Emden-Fowler equation in the continuous case to the dynamic equation (0.1). As applications, we show that the sublinear difference equation has a nonoscillatory solution, for b>0, c>[alpha], and the sublinear q-difference equation has a nonoscillatory solution, for , q>1, b>0, c>1+[alpha].

  17. Holographic Brownian motion and time scales in strongly coupled plasmas

    Energy Technology Data Exchange (ETDEWEB)

    Atmaja, Ardian Nata [Research Center for Physics, Indonesian Institute of Sciences (LIPI), Kompleks PUSPITEK Serpong, Tangerang 15310 (Indonesia); Indonesia Center for Theoretical and Mathematical Physics (ICTMP), Bandung 40132 (Indonesia); Boer, Jan de [Institute for Theoretical Physics, University of Amsterdam, Valckenierstraat 65, 1018 XE Amsterdam (Netherlands); Shigemori, Masaki [Yukawa Institute for Theoretical Physics (YITP), Kyoto University, Kitashirakawa Oiwakecho, Sakyo-ku, Kyoto 606-8502 (Japan); Hakubi Center, Kyoto University, Yoshida-Ushinomiyacho, Sakyo-ku, Kyoto 606-8501 (Japan)


    We study Brownian motion of a heavy quark in field theory plasma in the AdS/CFT setup and discuss the time scales characterizing the interaction between the Brownian particle and plasma constituents. Based on a simple kinetic theory, we first argue that the mean-free-path time is related to the connected 4-point function of the random force felt by the Brownian particle. Then, by holographically computing the 4-point function and regularizing the IR divergence appearing in the computation, we write down a general formula for the mean-free-path time, and apply it to the STU black hole which corresponds to plasma charged under three U(1)R-charges. The result indicates that the Brownian particle collides with many plasma constituents simultaneously.

  18. Liquidity spillover in international stock markets through distinct time scales. (United States)

    Righi, Marcelo Brutti; Vieira, Kelmara Mendes


    This paper identifies liquidity spillovers through different time scales based on a wavelet multiscaling method. We decompose daily data from U.S., British, Brazilian and Hong Kong stock markets indices in order to calculate the scale correlation between their illiquidities. The sample is divided in order to consider non-crisis, sub-prime crisis and Eurozone crisis. We find that there are changes in correlations of distinct scales and different periods. Association in finest scales is smaller than in coarse scales. There is a rise on associations in periods of crisis. In frequencies, there is predominance for significant distinctions involving the coarsest scale, while for crises periods there is predominance for distinctions on the finest scale.

  19. Empirical mode decomposition using variable filtering with time scale calibrating

    Institute of Scientific and Technical Information of China (English)


    A novel and efficient method for decomposing a signal into a set of intrinsic mode functions (IMFs) and a trend is proposed. Unlike the original empirical mode decomposition (EMD), which uses spline fits to extract variations from the signal by separating the local mean from the fluctuations in the decomposing process, this new method being proposed takes advantage of the theory of variable finite impulse response (FIR) filtering where filter coefficients and breakpoint frequencies can be adjusted to track any peak-to-peak time scale changes. The IMFs are results of a multiple variable frequency response FIR filtering when signals pass through the filters. Numerical examples validate that in contrast with the original EMD, the proposed method can fine-tune the frequency resolution and suppress the aliasing effectively.

  20. On the superposition of heterogeneous traffic at large time scales

    Directory of Open Access Journals (Sweden)

    Sidney I. Resnick


    Full Text Available Various empirical and theoretical studies indicate that cumulative network traffic is a Gaussian process. However, depending on whether the intensity at which sessions are initiated is large or small relative to the session duration tail, [25] and [15] have shown that traffic at large time scales can be approximated by either fractional Brownian motion (fBm or stable Lévy motion. We study distributional properties of cumulative traffic that consists of a finite number of independent streams and give an explanation of why Gaussian examples abound in practice but not stable Lévy motion. We offer an explanation of how much vertical aggregation is needed for the Gaussian approximation to hold. Our results are expressed as limit theorems for a sequence of cumulative traffic processes whose session initiation intensities satisfy growth rates similar to those used in [25].

  1. Two-time-scale population evolution on a singular landscape (United States)

    Xu, Song; Jiao, Shuyun; Jiang, Pengyao; Ao, Ping


    Under the effect of strong genetic drift, it is highly probable to observe gene fixation or gene loss in a population, shown by singular peaks on a potential landscape. The genetic drift-induced noise gives rise to two-time-scale diffusion dynamics on the bipeaked landscape. We find that the logarithmically divergent (singular) peaks do not necessarily imply infinite escape times or biological fixations by iterating the Wright-Fisher model and approximating the average escape time. Our analytical results under weak mutation and weak selection extend Kramers's escape time formula to models with B (Beta) function-like equilibrium distributions and overcome constraints in previous methods. The constructed landscape provides a coherent description for the bistable system, supports the quantitative analysis of bipeaked dynamics, and generates mathematical insights for understanding the boundary behaviors of the diffusion model.

  2. Estimating ventilation time scales using overturning stream functions (United States)

    Thompson, Bijoy; Nycander, Jonas; Nilsson, Johan; Jakobsson, Martin; Döös, Kristofer


    A simple method for estimating ventilation time scales from overturning stream functions is proposed. The stream function may be computed using either geometric coordinates or a generalized vertical coordinate, such as potential density (salinity in our study). The method is tested with a three-dimensional circulation model describing an idealized semi-enclosed ocean basin ventilated through a narrow strait over a sill, and the result is compared to age estimates obtained from a passive numerical age tracer. The best result is obtained when using the stream function in salinity coordinates. In this case, the reservoir-averaged advection time obtained from the overturning stream function in salinity coordinates agrees rather well with the mean age of the age tracer, and the corresponding maximum ages agree very well.

  3. A Review of Time-Scale Modification of Music Signals

    Directory of Open Access Journals (Sweden)

    Jonathan Driedger


    Full Text Available Time-scale modification (TSM is the task of speeding up or slowing down an audio signal’s playback speed without changing its pitch. In digital music production, TSM has become an indispensable tool, which is nowadays integrated in a wide range of music production software. Music signals are diverse—they comprise harmonic, percussive, and transient components, among others. Because of this wide range of acoustic and musical characteristics, there is no single TSM method that can cope with all kinds of audio signals equally well. Our main objective is to foster a better understanding of the capabilities and limitations of TSM procedures. To this end, we review fundamental TSM methods, discuss typical challenges, and indicate potential solutions that combine different strategies. In particular, we discuss a fusion approach that involves recent techniques for harmonic-percussive separation along with time-domain and frequency-domain TSM procedures.

  4. Variation of atmospheric depth profile on different time scales

    CERN Document Server

    Wilczynska, B; Homola, P; Pekala, J; Risse, M; Wilczynski, H


    The vertical profile of atmospheric depth is an important element in extensive air shower studies. The depth of shower maximum is one of the most important characteristics of the shower. In the fluorescence technique of shower detection, the geometrical reconstruction provides the altitude of shower maximum, so that an accurate profile of atmospheric depth is needed to convert this altitude to the depth of shower maximum. In this paper the temporal variation of experimentally measured profiles of atmospheric depth at different sites is studied and implications for shower reconstruction are shown. The atmospheric profiles vary on time scales from hours to years. It is shown that the daily variation of the profile is as important as its seasonal variation and should be accounted for in air shower studies. For precise shower reconstruction, the daily profiles determined locally at the site of the air shower detector are recommended.

  5. Terrestrial Waters and Sea Level Variations on Interannual Time Scale (United States)

    Llovel, W.; Becker, M.; Cazenave, A.; Jevrejeva, S.; Alkama, R.; Decharme, B.; Douville, H.; Ablain, M.; Beckley, B.


    On decadal to multi-decadal time scales, thermal expansion of sea waters and land ice loss are the main contributors to sea level variations. However, modification of the terrestrial water cycle due to climate variability and direct anthropogenic forcing may also affect sea level. For the past decades, variations in land water storage and corresponding effects on sea level cannot be directly estimated from observations because these are almost non-existent at global continental scale. However, global hydrological models developed for atmospheric and climatic studies can be used for estimating total water storage. For the recent years (since mid-2002), terrestrial water storage change can be directly estimated from observations of the GRACE space gravimetry mission. In this study, we analyse the interannual variability of total land water storage, and investigate its contribution to mean sea level variability at interannual time scale. We consider three different periods that, each, depend on data availability: (1) GRACE era (2003-2009), (2) 1993-2003 and (3) 1955-1995. For the GRACE era (period 1), change in land water storage is estimated using different GRACE products over the 33 largest river basins worldwide. For periods 2 and 3, we use outputs from the ISBA-TRIP (Interactions between Soil, Biosphere, and Atmosphere-Total Runoff Integrating Pathways) global hydrological model. For each time span, we compare change in land water storage (expressed in sea level equivalent) to observed mean sea level, either from satellite altimetry (periods 1 and 2) or tide gauge records (period 3). For each data set and each time span, a trend has been removed as we focus on the interannual variability. We show that whatever the period considered, interannual variability of the mean sea level is essentially explained by interannual fluctuations in land water storage, with the largest contributions arising from tropical river basins.

  6. Towards a stable numerical time scale for the early Paleogene (United States)

    Hilgen, Frederik; Kuiper, Klaudia; Sierro, Francisco J.; Wotzlaw, Jorn; Schaltegger, Urs; Sahy, Diana; Condon, Daniel


    The construction of an astronomical time scale for the early Paleogene is hampered by ambiguities in the number, correlation and tuning of 405-kyr eccentricity related cycles in deep marine records from ODP cores and land-based sections. The two most competing age models result in astronomical ages for the K/Pg boundary that differ by ~750 kyr (~66.0 Ma of Vandenberghe et al. (2012) versus 65.25 Ma of Westerhold et al. (2012); these ages in turn are consistent with proposed ages for the Fish Canyon sanidine (FCs) that differ by ~300 kyr (28.201 Ma of Kuiper et al. (2008) versus 27.89 Ma of Westerhold et al. (2012)); an even older age of 28.294 Ma is proposed based on a statistical optimization model (Renne et al., 2011). The astronomically calibrated FCs age of 28.201 ± 0.046 Ma of Kuiper et al. (2008), which is consistent with the astronomical age of ~66.0 Ma for the K/Pg boundary, is currently adopted in the standard geological time scale (GTS2012). Here we combine new and published data in an attempt to solve the controversy and arrive at a stable nuemrical time scale for the early Paleogene. Supporting their younger age model, Westerhold et al. (2012) argue that the tuning of Miocene sections in the Mediterranean, which underlie the older FCs age of Kuiper et al. (2008) and, hence, the coupled older early Paleogene age model of Vandenberghe et al. (2012), might be too old by three precession cycles. We thoroughly rechecked this tuning; distinctive cycle patterns related to eccentricity and precession-obliquity interference make a younger tuning that would be consistent with the younger astronomical age of 27.89 Ma for the FCs of Westerhold et al. (2012) challenging. Next we compared youngest U/Pb zircon and astronomical ages for a number of ash beds in the tuned Miocene section of Monte dei Corvi. These ages are indistinguishable, indicating that the two independent dating methods yield the same age when the same event is dated. This is consistent with results

  7. 液质联用多反应监测法定量目标多肽或蛋白质%Quantification of Target Peptides or Proteins by Liquid ChromatographyMass Spectrometry with Multiple Reaction Monitoring

    Institute of Scientific and Technical Information of China (English)

    刘永福; 贾小芳; 腾珍林; 尹林; 刘保池; 张丽军


    为建立优化的血浆内源性多肽提取方法,并且构建目标多肽和蛋白质的质谱定量方法,本研究考察了超滤法、有机溶剂沉淀法和固相萃取法对血浆内源性多肽的提取效果,并通过Tricine-SDS-PAGE对提取效果进行比较.通过液相色谱串联质谱多反应监测(MRM)分析,建立了多肽标准品ESAT-6定量方法,并将ESAT-6定量建立的液相色谱和质谱条件应用于蛋白质的定量,对多肽和蛋白质MRM定量的标准曲线进行了考察.Tricine-SDS-PAGE结果表明,乙腈沉淀法是最佳的血浆内源性多肽提取方法,低分子量的多肽可以得到很好的富集,且能有效地去除高分子蛋白质的污染.液相色谱串联质谱MRM法检测血浆内提取的多肽,标准曲线的线性较好,相关系数为0.999.另外,采用MRM法对胶内分离的蛋白质进行定量,标准曲线的线性相关系数为0.995.综上所述,本研究构建了一种简单有效的血浆多肽提取方法,通过液质联用MRM法成功地实现了目标多肽和蛋白质定量测定.该定量方法可以推广应用于复杂样品中的多肽和蛋白质的定量分析.%This study was to establish a modified plasma peptide extraction method and to develop a mass spectrometry quantification method for target peptides or proteins. Three techniques of ultrafiltration, organic solvent extraction and solid phase extraction were used to extract peptides from plasma samples. Tricine-SDS-PAGE was used to determine the extraction efficiency. Peptide and protein quantification were completed by liquid chromatography combined with mass spectrometry in multiple reaction monitoring (MRM) mode. The Tricine-SDS-PAGE results showed that acetonitrile (CAN) precipitation was the most efficient approach for low molecular plasma peptides enrichment besides for excluding the high molecular protein contaminations. The correlation coefficients of the standard curves were 0. 995 and 0. 999 in the quantification of

  8. Forecasting decadal and shorter time-scale solar cycle features (United States)

    Dikpati, Mausumi


    Solar energetic particles and magnetic fields reach the Earth through the interplanetary medium and affect it in various ways, producing beautiful aurorae, but also electrical blackouts and damage to our technology-dependent economy. The root of energetic solar outputs is the solar activity cycle, which is most likely caused by dynamo processes inside the Sun. It is a formidable task to accurately predict the amplitude, onset and peak timings of a solar cycle. After reviewing all solar cycle prediction methods, including empirical as well as physical model-based schemes, I will describe what we have learned from both validation and nonvalidation of cycle 24 forecasts, and how to refine the model-based schemes for upcoming cycle 25 forecasts. Recent observations indicate that within a solar cycle there are shorter time-scale 'space weather' features, such as bursts of various forms of activity with approximately one year periodicity. I will demonstrate how global tachocline dynamics could play a crucial role in producing such space weather. The National Center for Atmospheric Research is sponsored by the National Science Foundation.

  9. The quenching time scale and quenching rate of galaxies

    CERN Document Server

    Lian, Jianhui; Zhang, Kai; Kong, Xu


    The average star formation rate (SFR) in galaxies has been declining since redshift of 2. A fraction of galaxies quench and become quiescent. We constrain two key properties of the quenching process: the quenching time scale and the quenching rate among galaxies. We achieve this by analyzing the galaxy number density profile in NUV-u color space and the distribution in NUV-u v.s. u-i color-color diagram with a simple toy-model framework. We focus on galaxies in three mass bins between 10 to 10 and 10 to 10.6 solar mass. In the NUV-u v.s. u-i color-color diagram, the red u-i galaxies exhibit a different slope from the slope traced by the star-forming galaxies. This angled distribution and the number density profile of galaxies in NUV-u space strongly suggest that the decline of the SFR in galaxies has to accelerate before they turn quiescent. We model this color-color distribution with a two-phase exponential decline star formation history. The models with an e-folding time in the second phase (the quenching p...

  10. On the superimposition of heterogeneous traffic at large time scales

    CERN Document Server

    Lopez-Oliveros, Luis


    Various empirical and theoretical studies indicate that cumulative network traffic is a Gaussian process. However, depending on whether the intensity at which sessions are initiated is large or small relative to the session duration tail, Mikosch et a. (Ann Appl Probab, 12:23-68, 2002) and Kaj and Taqqu (Progress Probab, 60:383-427, 2008) have shown that traffic at large time scales can be approximated by either fractional Brownian motion (fBm) or stable Levy motion. We study distributional properties of cumulative traffic that consists of a finite number of independent streams and give an explanation of why Gaussian examples abound in practice but not stable Levy motion. We offer an explanation of how much vertical aggregation is needed for the Gaussian approximation to hold. Our results are expressed as limit theorems for a sequence of cumulative traffic processes whose session initiation intensities satisfy growth rates similar to those used in Mikosch et a. (Ann Appl Probab, 12:23-68, 2002).

  11. Selective attention to temporal features on nested time scales. (United States)

    Henry, Molly J; Herrmann, Björn; Obleser, Jonas


    Meaningful auditory stimuli such as speech and music often vary simultaneously along multiple time scales. Thus, listeners must selectively attend to, and selectively ignore, separate but intertwined temporal features. The current study aimed to identify and characterize the neural network specifically involved in this feature-selective attention to time. We used a novel paradigm where listeners judged either the duration or modulation rate of auditory stimuli, and in which the stimulation, working memory demands, response requirements, and task difficulty were held constant. A first analysis identified all brain regions where individual brain activation patterns were correlated with individual behavioral performance patterns, which thus supported temporal judgments generically. A second analysis then isolated those brain regions that specifically regulated selective attention to temporal features: Neural responses in a bilateral fronto-parietal network including insular cortex and basal ganglia decreased with degree of change of the attended temporal feature. Critically, response patterns in these regions were inverted when the task required selectively ignoring this feature. The results demonstrate how the neural analysis of complex acoustic stimuli with multiple temporal features depends on a fronto-parietal network that simultaneously regulates the selective gain for attended and ignored temporal features.

  12. Time scale of diffusion in molecular and cellular biology (United States)

    Holcman, D.; Schuss, Z.


    Diffusion is the driver of critical biological processes in cellular and molecular biology. The diverse temporal scales of cellular function are determined by vastly diverse spatial scales in most biophysical processes. The latter are due, among others, to small binding sites inside or on the cell membrane or to narrow passages between large cellular compartments. The great disparity in scales is at the root of the difficulty in quantifying cell function from molecular dynamics and from simulations. The coarse-grained time scale of cellular function is determined from molecular diffusion by the mean first passage time of molecular Brownian motion to a small targets or through narrow passages. The narrow escape theory (NET) concerns this issue. The NET is ubiquitous in molecular and cellular biology and is manifested, among others, in chemical reactions, in the calculation of the effective diffusion coefficient of receptors diffusing on a neuronal cell membrane strewn with obstacles, in the quantification of the early steps of viral trafficking, in the regulation of diffusion between the mother and daughter cells during cell division, and many other cases. Brownian trajectories can represent the motion of a molecule, a protein, an ion in solution, a receptor in a cell or on its membrane, and many other biochemical processes. The small target can represent a binding site or an ionic channel, a hidden active site embedded in a complex protein structure, a receptor for a neurotransmitter on the membrane of a neuron, and so on. The mean time to attach to a receptor or activator determines diffusion fluxes that are key regulators of cell function. This review describes physical models of various subcellular microdomains, in which the NET coarse-grains the molecular scale to a higher cellular-level, thus clarifying the role of cell geometry in determining subcellular function.

  13. Gas Chromatography (United States)

    Qian, Michael C.

    Gas chromatography (GC) has many applications in the analysis of food products. GC has been used for the determination of fatty acids, triglycerides, cholesterol, gases, water, alcohols, pesticides, flavor compounds, and many more. While GC has been used for other food components such as sugars, oligosaccharides, amino acids, peptides, and vitamins, these substances are more suited to analysis by high performance liquid chromatography. GC is ideally suited to the analysis of volatile substances that are thermally stable. Substances such as pesticides and flavor compounds that meet these criteria can be isolated from a food and directly injected into the GC. For compounds that are thermally unstable, too low in volatility, or yield poor chromatographic separation due to polarity, a derivatization step must be done before GC analysis. The two parts of the experiment described here include the analysis of alcohols that requires no derivatization step, and the analysis of fatty acids which requires derivatization. The experiments specify the use of capillary columns, but the first experiment includes conditions for a packed column.

  14. Application of at-line two-dimensional liquid chromatography-mass spectrometry for identification of small hydrophilic angiotensin I-inhibiting peptides in milk hydrolysates

    NARCIS (Netherlands)

    van Platerink, C.J.; Janssen, H.-G.M.; Haverkamp, J.


    A two-dimensional chromatographic method with mass spectrometric detection has been developed for identification of small, hydrophilic angiotensin I-inhibiting peptides in enzymatically hydrolysed milk proteins. The method involves the further separation of the poorly retained hydrophilic fraction f


    A new automated 2D-(SCX/RP)-nano-LC/MSMS method was developed. Separation of the peptides in the first LC dimension was the main focus of this work, and it was optimized using human serum albumin (HSA) and human lung cell lysate tryptic digests. Samples were reduced and alkylated...

  16. 色谱纯化和质谱分析法研究牛骨源咸味肽%Separation, Purification and Analysis of Salty Peptides Derived from Bovine Bone by Chromatography and Mass Spectrometry

    Institute of Scientific and Technical Information of China (English)

    李迎楠; 刘文营; 张顺亮; 成晓瑜


    以牛骨为研究对象,通过中空纤维超滤装置、Sephadex G-25凝胶色谱柱对相对分子质量小于5000的酶解产物进行初步分离,选用制备型及分析型高效液相色谱分析仪对咸味肽进行纯化、收集及分析。结果表明:收集得到牛骨源咸味肽为单一组分;用基质辅助激光解析电离-飞行时间质谱仪对咸味肽进行分析,得到其为相对分子质量均小于1000的短肽,其呈现咸味的物质质荷比值可能为679.5109。%The enzymatic hydrolysate of bovine bone with relative molecular weight < 5 000 was separated by hollow fiber ultrafiltration and Sephadex G-25 column chromatography. Two salty peptide fractions from the hydrolysate were purified by preparative high performance liquid chromatography (HPLC) and the pooled samples were analyzed by HPLC. The two fractions were found to consist of a single homogenous component. As analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS), both peptide fractions had a relative molecular weight less than 1000, the salty taste compound of which was m/z 679.510 9.

  17. Two-phase micro- and macro-time scales in particle-laden turbulent channel flows

    Institute of Scientific and Technical Information of China (English)

    Bing Wang; Michael Manhart


    The micro- and macro-time scales in two-phase turbulent channel flows are investigated using the direct numerical simulation and the Lagrangian particle trajectory methods for the fluid- and the particle-phases,respectively.Lagrangian and Eulerian time scales of both phases are calculated using velocity correlation functions.Due to flow anisotropy,micro-time scales are not the same with the theoretical estimations in large Reynolds number (isotropic) turbulence.Lagrangian macro-time scales of particle-phase and of fluid-phase seen by particles are both dependent on particle Stokes number.The fluid-phase Lagrangian integral time scales increase with distance from the wall,longer than those time scales seen by particles.The Eulerian integral macro-time scales increase in near-wall regions but decrease in out-layer regions.The moving Eulerian time scales are also investigated and compared with Lagrangian integral time scales,and in good agreement with previous measurements and numerical predictions.For the fluid particles the micro Eulerian time scales are longer than the Lagrangian ones in the near wall regions,while away from the walls the micro Lagrangian time scales are longer.The Lagrangian integral time scales are longer than the Eulerian ones.The results are useful for further understanding two-phase flow physics and especially for constructing accurate prediction models of inertial particle dispersion.

  18. Noether theorem for nonholonomic nonconservative mechanical systems in phase space on time scales (United States)

    Zu, Qi-hang; Zhu, Jian-qing


    The paper focuses on studying the Noether theorem for nonholonomic nonconservative mechanical systems in phase space on time scales. First, the Hamilton equations of nonholonomic nonconservative systems on time scales are established, which is based on the Lagrange equations for nonholonomic systems on time scales. Then, based upon the quasi-invariance of Hamilton action of systems under the infinitesimal transformations with respect to the time and generalized coordinate on time scale, the Noether identity and the conserved quantity of nonholonomic nonconservative systems on time scales are obtained. Finally, an example is presented to illustrate the application of the results.

  19. Multiple time scales and the lifetime coefficient of variation: engineering applications. (United States)

    Kordonsky, K B; Gertsbakh, I


    We consider linear combinations of "natural" time scales and choose the "best" one which provides the minimum coefficient of variation of the lifetime. Our time scale is in fact a generalized Miner time scale because the latter is based on an appropriate weighting of the times spent on low and high level loadings. The suggested modus operandi for finding the "best" time scale has many features in common with the approach suggested by Farewell and Cox (1979) and Oakes (1995) which is devoted to multiple time scales in survival analysis.

  20. Purification of polyclonal antibodies from Cohn fraction II + III, skim milk, and whey by affinity chromatography using a hexamer peptide ligand. (United States)

    Menegatti, Stefano; Naik, Amith D; Gurgel, Patrick V; Carbonell, Ruben G


    HWRGWV, a peptide that binds specifically to the Fc fragment of human immunoglobulin G (IgG), was used for the purification of IgG from Cohn fraction II + III of human plasma and from bovine skim milk and whey. The concentration of sodium chloride and sodium caprylate in the binding buffer as well as the pH of the elution buffer were optimized to achieve high IgG yield and purity. Under optimized conditions, IgG was recovered from plasma fractions with yield and purity up to 84% and 95%, respectively. IgG was also purified from skim milk with 74% yield and 92% purity and from whey with 85% yield and 93% purity. Purification experiments were also performed with Protein A resin and the results were found to be similar to those obtained with the peptide adsorbent.

  1. Investigation on enantiomeric separations of fluorenylmethoxycarbonyl amino acids and peptides by high-performance liquid chromatography using native cyclodextrins as chiral stationary phases. (United States)

    Tang, Y; Zukowski, J; Armstrong, D W


    A systematic study was carried out to investigate enantiomeric separations of fluorenylmethoxycarbonyl (FMOC) amino acids and their peptides. Twenty amino acids were derivatized by 9-fluorenylmethyl chloroformate (FMOC-Cl) and its analogues, FMOC-glycyl-Cl and FMOC-beta-alanyl-Cl. All derivatives were chromatographed on native beta- and gamma-cyclodextrin columns using acetonitrile as the main mobile phase component. The results indicated that glycyl and beta-alanyl groups between FMOC and amino acid moieties enhanced chiral selectivities of amino acid derivatives. The addition of modifiers, triethylamine, acetic acid and methanol, into the mobile phase caused alterations in retention, enantiorecognition and elution order. The structures of amino acids and the type of chiral stationary phase employed exhibited significant impacts on chiral resolutions. It is also found that the number and position of glycyl moieties affect the retentions and enantioselectivities of FMOC derivatized glycyl containing peptides.

  2. Input-output description of linear systems with multiple time-scales (United States)

    Madriz, R. S.; Sastry, S. S.


    It is pointed out that the study of systems evolving at multiple time-scales is simplified by studying reduced-order models of these systems valid at specific time-scales. The present investigation is concerned with an extension of results on the time-scale decomposition of autonomous systems to that of input-output systems. The results are employed to study conditions under which positive realness of a transfer function is preserved under singular perturbation. Attention is given to the perturbation theory for linear operators, the multiple time-scale structure of autonomous linear systems, the input-output description of two time-scale linear systems, the positive realness of two time-scale systems, and multiple time-scale linear systems.

  3. Simple setup for gas-phase H/D exchange mass spectrometry coupled to electron transfer dissociation and ion mobility for analysis of polypeptide structure on a liquid chromatographic time scale. (United States)

    Mistarz, Ulrik H; Brown, Jeffery M; Haselmann, Kim F; Rand, Kasper D


    Gas-phase hydrogen/deuterium exchange (HDX) is a fast and sensitive, yet unharnessed analytical approach for providing information on the structural properties of biomolecules, in a complementary manner to mass analysis. Here, we describe a simple setup for ND3-mediated millisecond gas-phase HDX inside a mass spectrometer immediately after ESI (gas-phase HDX-MS) and show utility for studying the primary and higher-order structure of peptides and proteins. HDX was achieved by passing N2-gas through a container filled with aqueous deuterated ammonia reagent (ND3/D2O) and admitting the saturated gas immediately upstream or downstream of the primary skimmer cone. The approach was implemented on three commercially available mass spectrometers and required no or minor fully reversible reconfiguration of gas-inlets of the ion source. Results from gas-phase HDX-MS of peptides using the aqueous ND3/D2O as HDX reagent indicate that labeling is facilitated exclusively through gaseous ND3, yielding similar results to the infusion of purified ND3-gas, while circumventing the complications associated with the use of hazardous purified gases. Comparison of the solution-phase- and gas-phase deuterium uptake of Leu-Enkephalin and Glu-Fibrinopeptide B, confirmed that this gas-phase HDX-MS approach allows for labeling of sites (heteroatom-bound non-amide hydrogens located on side-chains, N-terminus and C-terminus) not accessed by classical solution-phase HDX-MS. The simple setup is compatible with liquid chromatography and a chip-based automated nanoESI interface, allowing for online gas-phase HDX-MS analysis of peptides and proteins separated on a liquid chromatographic time scale at increased throughput. Furthermore, online gas-phase HDX-MS could be performed in tandem with ion mobility separation or electron transfer dissociation, thus enabling multiple orthogonal analyses of the structural properties of peptides and proteins in a single automated LC-MS workflow.

  4. Implementation of Time-Scale Transformation Based on Continuous Wavelet Theory

    Institute of Scientific and Technical Information of China (English)


    The basic objective of time-scale transformation is to compress or expand the signal in time field while keeping the same spectral properties.This paper presents two methods to derive time-scale transformation formula based on continuous wavelet transform.For an arbitrary given square-integrable function f(t),g(t) = f(t/λ) is derived by continuous wavelet transform and its inverse transform.The result shows that time-scale transformation may be obtained through the modification of the time-scale of wavelet function filter using equivalent substitution. The paper demonstrates the result by theoretic derivations and experimental simulation.

  5. Applying Chromatography. (United States)

    Klein, Jessie W.; Patev, Paul


    Presents three experiments to introduce students to different kinds of chromatography: (1) paper chromatography; (2) gel filtration chromatography; and (3) reverse-phase liquid chromatography. Written in the form of a laboratory manual, explanations of each of the techniques, materials needed, procedures, and a glossary are included. (PVD)

  6. Holder's and Hardy's Two Dimensional Diamond-alpha Inequalities on Time Scales

    CERN Document Server

    Ammi, Moulay Rchid Sidi


    We prove a two dimensional Holder and reverse-Holder inequality on time scales via the diamond-alpha integral. Other integral inequalities are established as well, which have as corollaries some recent proved Hardy-type inequalities on time scales.

  7. Unique Existence Theorem of Solution of Almost Periodic Differential Equations on Time Scales

    Directory of Open Access Journals (Sweden)

    Meng Hu


    Full Text Available By using the theory of calculus on time scales and M-matrix theory, the unique existence theorem of solution of almost periodic differential equations on almost periodic time scales is established. The result can be used to a large of dynamic systems.

  8. Unique Existence Theorem of Solution of Almost Periodic Differential Equations on Time Scales


    Meng Hu; Lili Wang


    By using the theory of calculus on time scales and M-matrix theory, the unique existence theorem of solution of almost periodic differential equations on almost periodic time scales is established. The result can be used to a large of dynamic systems.

  9. Error estimates for asymptotic solutions of dynamic equations on time scales

    Directory of Open Access Journals (Sweden)

    Gro Hovhannisyan


    Full Text Available We establish error estimates for first-order linear systems of equations and linear second-order dynamic equations on time scales by using calculus on a time scales [1,4,5] and Birkhoff-Levinson's method of asymptotic solutions [3,6,8,9].

  10. Statistics of bedload transport over steep slopes: Separation of time scales and collective motion

    CERN Document Server

    Heyman, J; Ma, H B; Ancey, C


    Steep slope streams show large fluctuations of sediment discharge across several time scales. These fluctuations may be inherent to the internal dynamics of the sediment transport process. A probabilistic framework thus seems appropriate to analyze such a process. In this letter, we present an experimental study of bedload transport over a steep slope flume for small to moderate Shields numbers. The sampling technique allows the acquisition of high-resolution time series of the solid discharge. The resolved time scales range from $10^{-2}$s up to $10^{5}$s. We show that two distinct time scales can be observed in the probability density function for the waiting time between moving particles. We make the point that the separation of time scales is related to collective dynamics. Proper statistics of a Markov process including collective entrainment are derived. The separation of time scales is recovered theoretically for low entrainment rates.

  11. Global and Local Color Time Scales to Encode Timeline Events in Ion Trajectories for Glassies

    Directory of Open Access Journals (Sweden)

    J. M. Sharif


    Full Text Available Glassy compounds lead directly to high ionic conductivity. Ionic conductivity generates ion trajectories. However, these trajectories have been represented by two-dimensional graph in order to visualize the timeline events in ion trajectories. This study addresses this problem by encoding the timeline events in ion trajectories with global and local color scales. Two time scales have been introduced namely Global Color Time Scale and Local Color Time Scale. The rainbow color has been chosen to represent global time scale meanwhile solid color has been used to generate local time scale. Based on evaluation, these techniques are successful in representing timeline events in ion trajectories for understanding the complicated heterogeneous movement of ion trajectories.

  12. Quantitative profiling of PrP(Sc) peptides by high-performance liquid chromatography mass spectrometry to investigate the diversity of prions. (United States)

    Gielbert, Adriana; Davis, Linda A; Sayers, A Robin; Tang, Yue; Hope, James; Sauer, Maurice J


    Prions are proteins that can exist in two (or more) folding states, a normal or cellular form and a series of infectious or prion forms, which are prone to aggregate. The prion form can induce conversion of the cellular form and so transmit phenotypic effects of this structural rearrangement within and between cells and organisms. The conversion of PrP(C), the mammalian prion glycoprotein, to its prion form, PrP(Sc), in the brain is a precursor to progressive neurological degeneration, and the various folded forms of PrP(Sc) (defined by the size and glycosylation of protease-resistant core peptides of the PrP aggregates, PrP(res)) are characteristic of a particular neurodegenerative phenotype or prion disease. Here, quantitative multiplex mass spectrometry was used for N-terminal amino acid profiling (N-TAAP) of PrP(res) from sheep affected by scrapie, the prion disease of small ruminants, to rapidly assess the diversity of prions within particular flocks. In 29 cases, PrP(res) concentrations varied from below the limit of detection (350 fmol/g) to 15 pmol/g wet brain. Although most had a single N-TAAP profile, two novel variants were identified: one common to the ARH/ARQ animals in this study and one in an animal of the wild-type sheep PrP genotype (ARQ/ARQ).

  13. An antifungal peptide from baby lima bean. (United States)

    Wang, H X; Ng, T B


    A 6-kDa antifungal peptide with inhibitory activity on mycelial growth in Fusarium oxysporum, Mycosphaerella arachidicola, and Physalospora piricola was isolated from baby lima beans. The peptide suppressed growth in M. arachidicola with an IC(50) of 0.87 muM and inhibited activity of HIV-1 reverse transcriptase with an IC(50) of 4 muM. The peptide exhibited an N-terminal amino acid sequence similar to those of leguminous defensins. The isolation procedure comprised ion exchange chromatography on diethylaminoethyl (DEAE)-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on carboxymethyl (CM)-cellulose, and gel filtration by fast protein liquid chromatography on Superdex 75. The peptide was unadsorbed on DEAE-cellulose and Affi-gel blue gel but was adsorbed on CM-cellulose.

  14. Global Exponential Stability of Delayed Cohen-Grossberg BAM Neural Networks with Impulses on Time Scales

    Directory of Open Access Journals (Sweden)

    Fei Yu


    Full Text Available Based on the theory of calculus on time scales, the homeomorphism theory, Lyapunov functional method, and some analysis techniques, sufficient conditions are obtained for the existence, uniqueness, and global exponential stability of the equilibrium point of Cohen-Grossberg bidirectional associative memory (BAM neural networks with distributed delays and impulses on time scales. This is the first time applying the time-scale calculus theory to unify the discrete-time and continuous-time Cohen-Grossberg BAM neural network with impulses under the same framework.

  15. Uniformly Almost Periodic Functions and Almost Periodic Solutions to Dynamic Equations on Time Scales

    Directory of Open Access Journals (Sweden)

    Yongkun Li


    Full Text Available Firstly, we propose a concept of uniformly almost periodic functions on almost periodic time scales and investigate some basic properties of them. When time scale T=ℝ or ℤ, our definition of the uniformly almost periodic functions is equivalent to the classical definitions of uniformly almost periodic functions and the uniformly almost periodic sequences, respectively. Then, based on these, we study the existence and uniqueness of almost periodic solutions and derive some fundamental conditions of admitting an exponential dichotomy to linear dynamic equations. Finally, as an application of our results, we study the existence of almost periodic solutions for an almost periodic nonlinear dynamic equations on time scales.


    Institute of Scientific and Technical Information of China (English)


    In this paper, using the theory of topological degree and Liapunov functional methods, the authors study the competitive neural networks with time delays and different time scales and present some criteria of global robust stability for this neural network model.

  17. Oscillation Criteria for Fourth-Order Nonlinear Dynamic Equations on Time Scales

    Directory of Open Access Journals (Sweden)

    Xin Wu


    Full Text Available We establish some new oscillation criteria for nonlinear dynamic equation of the form on an arbitrary time scale with , where are positive rd-continuous functions. An example illustrating the importance of our result is included.

  18. Freshwater flushing time scales of the Vashishti Estuary, west coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    DineshKumar, P.K.; Sarma, R.V.; Zingde, M.D.

    Results are presented for the Vashishti estuary, Kerala, India to evaluate its freshwater flushing time scales based on 8 sets of observations of longitudinal salinity distributions. The results of the flushing time using the fraction of freshwater...

  19. Time-scale invariances in preseismic electromagnetic radiation, magnetization and damage evolution of rocks

    Directory of Open Access Journals (Sweden)

    Y. Kawada


    Full Text Available We investigate the time-scale invariant changes in electromagnetic and mechanical energy releases prior to a rock failure or a large earthquake. The energy release processes are caused by damage evolutions such as crack propagation, motion of charged dislocation, area-enlargement of sheared asperities and repetitive creep-rate changes. Damage mechanics can be used to represent the time-scale invariant evolutions of both brittle and plastic damages. Irreversible thermodynamics applied to the damage mechanics reveals that the damage evolution produces the variations in charge, dipole and electromagnetic signals in addition to mechanical energy release, and yields the time-scale invariant patterns of Benioff electromagnetic radiation and cumulative Benioff strain-release. The irreversible thermodynamic framework of damage mechanics is also applicable to the seismo-magnetic effect, and the time-scale invariance is recognized in the remanent magnetization change associated with damage evolution prior to a rock failure.

  20. A Study on Time-Scales Ratio and Turbulent Prandtl Number in Ducts of Industrial Applications

    DEFF Research Database (Denmark)

    Rokni, Masoud


    This investigation concerns numerical time-scales ratio and turbulent Prandtl number in fully developed turbulent flows in ducts of various cross-sections. The low Reynolds number version of a non-linear eddy viscosity model is proposed to predict the Reynolds stresses and the temperature field...... is solved using a two-equation heat flux model. The computed results compare satisfactory with the available experimental data. The time-scale ratio R is defined as the ratio between the dynamic time-scale (k/ε) and the scalar time-scale(0.5θθ/εθ). Based on existing DNS data and calculations in this work...

  1. Multi-time scale analysis of precipitation variation in Guyuan,China:1957-2005

    Institute of Scientific and Technical Information of China (English)


    Morlet wavelet transformation is used in this paper to analyze the multi-time scale characteristics of precipitation data series from 1957 to 2005 in Guyuan region.The results showed that (1) the annual precipitation evolution process had obvious multi-time scale variation characteristics of 15-25 years,7-12 years and 3-6 years,and different time scales had different oscillation energy densities;(2) the periods at smaller time scales changed more frequently,which often nested in a biggish quasi periodic oscillations,so the concrete time domain should be analyzed if necessary;(3) the precipitation had three main periods (22-year,9-year and 4-year) and the 22-year period was especially outstanding,and the analysis of this main period reveals that the precipitation would be in a relative high water period until about 2012.

  2. Time-scaled scenario of low-energy heavy-ion collisions

    CERN Document Server

    Iwata, Yoritaka


    The underlying scenario of low-energy heavy-ion collisions is presented based on time-dependent density-functional calculations. A classification of several types of reaction dynamics is given with respect to their time-scales.

  3. Empirical study on structural properties in temporal networks under different time scales (United States)

    Chen, Duanbing


    Many network analyzing methods are usually based on static networks. However, temporal networks should be considered so as to investigate real complex systems deeply since some dynamics on these systems cannot be described by static networks accurately. In this paper, four structural properties in temporal networks are empirically studied, including degree, clustering coefficient, adjacent correlation, and connected component. Three real temporal networks with different time scales are analyzed in this paper, including short message, telephone, and router networks. Moreover, structural properties of these temporal networks are compared with that of corresponding static aggregation networks in the whole time window. Some essential differences of structural properties between temporal and static networks are achieved through empirical analysis. Finally, the effect of structural properties on spreading dynamics under different time scales is investigated. Some interesting results such as turning point of structure evolving time scale corresponding to certain spreading dynamics time scale from the point of view of infected scale are achieved.

  4. Neuere Chromatographie (United States)

    Hostettmann, K.


    Besides high-performance liquid chromatography (HPLC) which is now a well-established and currently used technique, several emerging methods for the isolation and separation of natural products are receiving considerable attention. Centrifugal thin-layer chromatography is a very rapid technique, but limited in resolution. Of special interest are the recently developed support-free liquid-liquid chromatography methods such as droplet counter-current chromatography (DCCC) and rotation locular counter-current chromatography (RLCC). This latter method was applied to the separation of the enantiomers of (±)-norephedrine.


    Institute of Scientific and Technical Information of China (English)


    We first investigate some basic properties of dynamic equations on time scales,and propose contained curves to describe the jump direction of the discrete points.Then we perform qualitative analysis regarding the planar predator-prey systems on time scales,thereby obtain two theorems of this system.At last,we emulate application examples to discuss the parameters of the system.

  6. Time-scale invariances in preseismic electromagnetic radiation, magnetization and damage evolution of rocks


    Kawada, Y.; H. Nagahama; Nakamura, N.


    International audience; We investigate the time-scale invariant changes in electromagnetic and mechanical energy releases prior to a rock failure or a large earthquake. The energy release processes are caused by damage evolutions such as crack propagation, motion of charged dislocation, area-enlargement of sheared asperities and repetitive creep-rate changes. Damage mechanics can be used to represent the time-scale invariant evolutions of both brittle and plastic damages. Irreversible thermod...

  7. Physical time scale in kinetic Monte Carlo simulations of continuous-time Markov chains. (United States)

    Serebrinsky, Santiago A


    We rigorously establish a physical time scale for a general class of kinetic Monte Carlo algorithms for the simulation of continuous-time Markov chains. This class of algorithms encompasses rejection-free (or BKL) and rejection (or "standard") algorithms. For rejection algorithms, it was formerly considered that the availability of a physical time scale (instead of Monte Carlo steps) was empirical, at best. Use of Monte Carlo steps as a time unit now becomes completely unnecessary.

  8. Oscillation of Second-order Nonlinear Dynamic Equation on Time Scales

    Institute of Scientific and Technical Information of China (English)

    YANG Jia-shan


    The oscillation for a class of second order nonlinear variable delay dynamic equation on time scales with nonlinear neutral term and damping term was discussed in this article.By using the generalized Riccati technique,integral averaging technique and the time scales theory,some new sufficient conditions for oscillation of the equation are proposed.These results generalize and extend many known results for second order dynamic equations.Some examples are given to illustrate the main results of this article.

  9. Estimating the distribution of rest-frame time-scales for blazar jets: a statistical approach (United States)

    Liodakis, I.; Blinov, D.; Papadakis, I.; Pavlidou, V.


    In any flux-density limited sample of blazars, the distribution of the time-scale modulation factor Δt΄/Δt, which quantifies the change in observed time-scales compared to the rest-frame ones due to redshift and relativistic compression follows an exponential distribution with a mean depending on the flux limit of the sample. In this work, we produce the mathematical formalism that allows us to use this information in order to uncover the underlining rest-frame probability density function of measurable time-scales of blazar jets. We extensively test our proposed methodology using a simulated Flat Spectrum Radio Quasar population with a 1.5 Jy flux-density limit in the simple case (where all blazars share the same intrinsic time-scale), in order to identify limits of applicability and potential biases due to observational systematics and sample selection. We find that for monitoring with time intervals between observations longer than ∼30 per cent of the intrinsic time-scale under investigation the method loses its ability to produce robust results. For time intervals of ∼3 per cent of the intrinsic time-scale, the error of the method is as low as 1 per cent in recovering the intrinsic rest-frame time-scale. We applied our method to rotations of the optical polarization angle of blazars observed by RoboPol. We found that the intrinsic time-scales of the longest duration rotation event in each blazar follows a narrow distribution, well described by a normal distribution with mean 87 d and standard deviation 5 d. We discuss possible interpretations of this result.

  10. Combined use of meteorological drought indices at multi-time scales for improving hydrological drought detection. (United States)

    Zhu, Ye; Wang, Wen; Singh, Vijay P; Liu, Yi


    Prediction of hydrological drought in the absence of hydrological records is of great significance for water resources management and risk assessment. In this study, two meteorological drought indices, including standardized precipitation index (SPI) and standardized precipitation evapotranspiration index (SPEI) calculated at different time scales (1 to 12months), were analyzed for their capabilities in detecting hydrological droughts. The predictive skills of meteorological drought indices were assessed through correlation analysis, and two skill scores, i.e. probability of detection (POD) and false alarm rate (FAR). When used independently, indices of short time scales generally performed better than did those of long time scales. However, at least 31% of hydrological droughts were still missed in view of the peak POD score (0.69) of a single meteorological drought index. Considering the distinguished roles of different time scales in explaining hydrological droughts with disparate features, an optimization approach of blending SPI/SPEI at multiple time scales was proposed. To examine the robustness of the proposed method, data of 1964-1990 was used to establish the multiscalar index, then validate during 2000-2010. Results showed that POD exhibited a significant increase when more than two time scales were used, and the best performances were found when blending 8 time scales of SPI and 9 for SPEI, with the corresponding values of 0.82 and 0.85 for POD, 0.205 and 0.21 for FAR, in the calibration period, and even better performance in the validation period. These results far exceeded the performance of any single meteorological drought index. This suggests that when there is lack of streamflow measurements, blending climatic information of multiple time scales to jointly monitor hydrological droughts could be an alternative solution.

  11. Time scales of porphyry Cu deposit formation: insights from titanium diffusion in quartz (United States)

    Mercer, Celestine N.; Reed, Mark H.; Mercer, Cameron M.


    Porphyry dikes and hydrothermal veins from the porphyry Cu-Mo deposit at Butte, Montana, contain multiple generations of quartz that are distinct in scanning electron microscope-cathodoluminescence (SEM-CL) images and in Ti concentrations. A comparison of microprobe trace element profiles and maps to SEM-CL images shows that the concentration of Ti in quartz correlates positively with CL brightness but Al, K, and Fe do not. After calibrating CL brightness in relation to Ti concentration, we use the brightness gradient between different quartz generations as a proxy for Ti gradients that we model to determine time scales of quartz formation and cooling. Model results indicate that time scales of porphyry magma residence are ~1,000s of years and time scales from porphyry quartz phenocryst rim formation to porphyry dike injection and cooling are ~10s of years. Time scales for the formation and cooling of various generations of hydrothermal vein quartz range from 10s to 10,000s of years. These time scales are considerably shorter than the ~0.6 m.y. overall time frame for each porphyry-style mineralization pulse determined from isotopic studies at Butte, Montana. Simple heat conduction models provide a temporal reference point to compare chemical diffusion time scales, and we find that they support short dike and vein formation time scales. We interpret these relatively short time scales to indicate that the Butte porphyry deposit formed by short-lived episodes of hydrofracturing, dike injection, and vein formation, each with discrete thermal pulses, which repeated over the ~3 m.y. generation of the deposit.

  12. Global Uniform Asymptotic Stability of Competitive Neural Networks with Different-Time Scales and Delay

    Institute of Scientific and Technical Information of China (English)

    LI Hong; L(U) Shu; ZHONG Shou-ming


    The global uniform asymptotic stability of competitive neural networks with different time scales and delay is investigated. By the method of variation of parameters and the method of inequality analysis, the condition for global uniformly asymptotically stable are given. A strict Lyapunov function for the flow of a competitive neural system with different time scales and delay is presented. Based on the function, the global uniform asymptotic stability of the equilibrium point can be proved.

  13. A two-time-scale autopilot for high-performance aircraft (United States)

    Menon, P. K. A.; Chatterji, G. B.; Cheng, V. H. L.


    A two-time-scale autopilot is proposed for the Aircraft Controls Design Challenge problem. This control law uses a nonlinear aircraft model constructed from the given vehicle simulation. The vehicle model is partitioned into slow translational dynamics and fast rotational dynamics. Feedback linearization is then employed to synthesize control laws for these two-time scales. Due to the nature of the synthesis, the control law is suitable for automatic trajectory following, and also for pilot control.

  14. Simultaneous enrichment of cysteine-containing peptides and phosphopeptides using a cysteine-specific phosphonate adaptable tag (CysPAT) in combination with titanium dioxide (TiO2) chromatography

    DEFF Research Database (Denmark)

    Huang, Honggang; Pedersen, Martin Haar; Ibañez-Vea, Maria


    to selectively label cysteine-containing peptides (Cys peptides) followed by their enrichment with titanium dioxide (TiO2) and subsequent mass spectrometric analysis. The CysPAT strategy was developed using a synthetic peptide, a standard protein and subsequently the strategy was applied to protein lysates from...

  15. Insights from inside the spinodal: Bridging thermalization time scales with smoothed particle hydrodynamics (United States)

    Pütz, Martin; Nielaba, Peter


    We report the influence of the strength of heat bath coupling on the demixing behavior in spinodal decomposing one component liquid-vapor systems. The smoothed particle hydrodynamics (SPH) method with a van der Waals equation of state is used for the simulation. A thermostat for SPH is introduced that is based on the Berendsen thermostat. It controls the strength of heat bath coupling and allows for quenches with exponential temperature decay at a certain thermalization time scale. The present method allows us to bridge several orders of magnitude in the thermalization time scale. The early stage is highly affected by the choice of time scale. A transition from exponential growth to a 1 /2 ordinary power law scaling in the characteristic lengths is observed. At high initial temperatures the growth is logarithmic. The comparison with pure thermal simulations reveals latent heat to raise the mean system temperature. Large thermalization time scales and thermal conductivity are figured out to affect a stagnation of heating, which is explained with convective processes. Furthermore, large thermalization time scales are responsible for a stagnation of growth of domains, which is temporally embedded between early and late stage of phase separation. Therefore, it is considered as an intermediate stage. We present an aspect concerning this stage, namely that choosing larger thermalization time scales increases the duration. Moreover, it is observed that diffuse interfaces are formed during this stage, provided that the stage is apparent. We show that the differences in the evolution between pure thermal simulations and simulations with an instantaneously scaled mean temperature can be explained by the thermalization process, since a variation of the time scale allows for the bridging between these cases of limit.

  16. A multi-time scale approach to remaining useful life prediction in rolling bearing (United States)

    Qian, Yuning; Yan, Ruqiang; Gao, Robert X.


    This paper presents a novel multi-time scale approach to bearing defect tracking and remaining useful life (RUL) prediction, which integrates enhanced phase space warping (PSW) with a modified Paris crack growth model. As a data-driven method, PSW describes the dynamical behavior of the bearing being tested on a fast-time scale, whereas the Paris crack growth model, as a physics-based model, characterizes the bearing's defect propagation on a slow-time scale. Theoretically, PSW constructs a tracking metric by evaluating the phase space trajectory warping of the bearing vibration data, and establishes a correlation between measurement on a fast-time scale and defect growth variables on a slow-time scale. Furthermore, PSW is enhanced by a multi-dimensional auto-regression (AR) model for improved accuracy in defect tracking. Also, the Paris crack growth model is modified by a time-piecewise algorithm for real-time RUL prediction. Case studies performed on two run-to-failure experiments indicate that the developed technique is effective in tracking the evolution of bearing defects and accurately predict the bearing RUL, thus contributing to the literature of bearing prognosis .

  17. Time-scales of close-in exoplanet radio emission variability (United States)

    See, V.; Jardine, M.; Fares, R.; Donati, J.-F.; Moutou, C.


    We investigate the variability of exoplanetary radio emission using stellar magnetic maps and 3D field extrapolation techniques. We use a sample of hot Jupiter hosting stars, focusing on the HD 179949, HD 189733 and τ Boo systems. Our results indicate two time-scales over which radio emission variability may occur at magnetized hot Jupiters. The first is the synodic period of the star-planet system. The origin of variability on this time-scale is the relative motion between the planet and the interplanetary plasma that is corotating with the host star. The second time-scale is the length of the magnetic cycle. Variability on this time-scale is caused by evolution of the stellar field. At these systems, the magnitude of planetary radio emission is anticorrelated with the angular separation between the subplanetary point and the nearest magnetic pole. For the special case of τ Boo b, whose orbital period is tidally locked to the rotation period of its host star, variability only occurs on the time-scale of the magnetic cycle. The lack of radio variability on the synodic period at τ Boo b is not predicted by previous radio emission models, which do not account for the co-rotation of the interplanetary plasma at small distances from the star.

  18. Time-scales of close-in exoplanet radio emission variability

    CERN Document Server

    See, V; Fares, R; Donati, J -F; Moutou, C


    We investigate the variability of exoplanetary radio emission using stellar magnetic maps and 3D field extrapolation techniques. We use a sample of hot Jupiter hosting stars, focusing on the HD 179949, HD 189733 and tau Boo systems. Our results indicate two time-scales over which radio emission variability may occur at magnetised hot Jupiters. The first is the synodic period of the star-planet system. The origin of variability on this time-scale is the relative motion between the planet and the interplanetary plasma that is co-rotating with the host star. The second time-scale is the length of the magnetic cycle. Variability on this time-scale is caused by evolution of the stellar field. At these systems, the magnitude of planetary radio emission is anticorrelated with the angular separation between the subplanetary point and the nearest magnetic pole. For the special case of tau Boo b, whose orbital period is tidally locked to the rotation period of its host star, variability only occurs on the time-scale of...

  19. Micro- and nano- second time scale, high power electrical wire explosions in water. (United States)

    Grinenko, Alon; Efimov, Sergey; Sayapin, Arkadii; Fedotov, Alexander; Gurovich, Viktor; Krasik, Yakov


    Experimental and magneto-hydro-dynamic simulation results of micro- and nanosecond time scale underwater electrical Al, Cu and W wires explosions are presented. A capacitor bank with stored energy up to 6 kJ (discharge current up to 80 kA with 2.5 μs quarter period) was used in microsecond time scale experiments and water forming line generator with current amplitude up to 100 kA and pulse duration of 100 ns were used in nanosecond time scale experiments. Extremely high energy deposition of up to 60 times the atomization enthalpy was registered in nanosecond time scale explosions. A discharge channel evolution and surface temperature were analyzed by streak shadow imaging and using fast photo-diode with a set of interference filters, respectively. Microsecond time scale electrical explosion of cylindrical wire array showed extremely high pressure of converging shock waves at the axis, up to 0.2 MBar. A 1D and 2D magneto-hydro-dynamic simulation demonstrated good agreement with such experimental parameters as discharge channel current, voltage, radius, and temperature.

  20. Interplay between multiple length and time scales in complex chemical systems

    Indian Academy of Sciences (India)

    Biman Bagchi; Charusita Chakravarty


    Processes in complex chemical systems, such as macromolecules, electrolytes, interfaces, micelles and enzymes, can span several orders of magnitude in length and time scales. The length and time scales of processes occurring over this broad time and space window are frequently coupled to give rise to the control necessary to ensure specificity and the uniqueness of the chemical phenomena. A combination of experimental, theoretical and computational techniques that can address a multiplicity of length and time scales is required in order to understand and predict structure and dynamics in such complex systems. This review highlights recent experimental developments that allow one to probe structure and dynamics at increasingly smaller length and time scales. The key theoretical approaches and computational strategies for integrating information across time-scales are discussed. The application of these ideas to understand phenomena in various areas, ranging from materials science to biology, is illustrated in the context of current developments in the areas of liquids and solvation, protein folding and aggregation and phase transitions, nucleation and self-assembly.

  1. Long-time scale spectral diffusion in PMMA: Beyond the TLS model? (United States)

    Müller, J.; Haarer, D.; Khodykin, O. V.; Kharlamov, B. M.


    Spectral diffusion (SD) in PMMA doped with H 2-TPP is investigated at 4.2 K on a time scale of 3 ÷ 10 6 s via optical hole burning. Two contradictory (in frames of the TLS model) results are obtained. The first is the absence of aging effects which put the upper limit for the TLS relaxation times to tens of minutes. The second is an intensive superlogarithmic SD on the whole time scale of the experiment, which evidences the presence of very slow relaxations, independent of the sample history on the time scale of up to 2 months. The presented results provide the clear evidence of the deviation of SD behavior from the TLS model predictions at moderately low temperatures. The concept of structural relaxations is applied for a qualitative interpretation of the experimental data.

  2. Response of vegetation to drought time-scales across global land biomes. (United States)

    Vicente-Serrano, Sergio M; Gouveia, Célia; Camarero, Jesús Julio; Beguería, Santiago; Trigo, Ricardo; López-Moreno, Juan I; Azorín-Molina, César; Pasho, Edmond; Lorenzo-Lacruz, Jorge; Revuelto, Jesús; Morán-Tejeda, Enrique; Sanchez-Lorenzo, Arturo


    We evaluated the response of the Earth land biomes to drought by correlating a drought index with three global indicators of vegetation activity and growth: vegetation indices from satellite imagery, tree-ring growth series, and Aboveground Net Primary Production (ANPP) records. Arid and humid biomes are both affected by drought, and we suggest that the persistence of the water deficit (i.e., the drought time-scale) could be playing a key role in determining the sensitivity of land biomes to drought. We found that arid biomes respond to drought at short time-scales; that is, there is a rapid vegetation reaction as soon as water deficits below normal conditions occur. This may be due to the fact that plant species of arid regions have mechanisms allowing them to rapidly adapt to changing water availability. Humid biomes also respond to drought at short time-scales, but in this case the physiological mechanisms likely differ from those operating in arid biomes, as plants usually have a poor adaptability to water shortage. On the contrary, semiarid and subhumid biomes respond to drought at long time-scales, probably because plants are able to withstand water deficits, but they lack the rapid response of arid biomes to drought. These results are consistent among three vegetation parameters analyzed and across different land biomes, showing that the response of vegetation to drought depends on characteristic drought time-scales for each biome. Understanding the dominant time-scales at which drought most influences vegetation might help assessing the resistance and resilience of vegetation and improving our knowledge of vegetation vulnerability to climate change.

  3. Enhanced identification and exploitation of time scales for model reduction in stochastic chemical kinetics. (United States)

    Gómez-Uribe, Carlos A; Verghese, George C; Tzafriri, Abraham R


    Widely different time scales are common in systems of chemical reactions and can be exploited to obtain reduced models applicable to the time scales of interest. These reduced models enable more efficient computation and simplify analysis. A classic example is the irreversible enzymatic reaction, for which separation of time scales in a deterministic mass action kinetics model results in approximate rate laws for the slow dynamics, such as that of Michaelis-Menten. Recently, several methods have been developed for separation of slow and fast time scales in chemical master equation (CME) descriptions of stochastic chemical kinetics, yielding separate reduced CMEs for the slow variables and the fast variables. The paper begins by systematizing the preliminary step of identifying slow and fast variables in a chemical system from a specification of the slow and fast reactions in the system. The authors then present an enhanced time-scale-separation method that can extend the validity and improve the accuracy of existing methods by better accounting for slow reactions when equilibrating the fast subsystem. The resulting method is particularly accurate in systems such as enzymatic and protein interaction networks, where the rates of the slow reactions that modify the slow variables are not a function of the slow variables. The authors apply their methodology to the case of an irreversible enzymatic reaction and show that the resulting improvements in accuracy and validity are analogous to those obtained in the deterministic case by using the total quasi-steady-state approximation rather than the classical Michaelis-Menten. The other main contribution of this paper is to show how mass fluctuation kinetics models, which give approximate evolution equations for the means, variances, and covariances of the concentrations in a chemical system, can feed into time-scale-separation methods at a variety of stages.

  4. Multiple time scales in modeling the incidence of infections acquired in intensive care units

    Directory of Open Access Journals (Sweden)

    Martin Wolkewitz


    Full Text Available Abstract Background When patients are admitted to an intensive care unit (ICU their risk of getting an infection will be highly depend on the length of stay at-risk in the ICU. In addition, risk of infection is likely to vary over calendar time as a result of fluctuations in the prevalence of the pathogen on the ward. Hence risk of infection is expected to depend on two time scales (time in ICU and calendar time as well as competing events (discharge or death and their spatial location. The purpose of this paper is to develop and apply appropriate statistical models for the risk of ICU-acquired infection accounting for multiple time scales, competing risks and the spatial clustering of the data. Methods A multi-center data base from a Spanish surveillance network was used to study the occurrence of an infection due to Methicillin-resistant Staphylococcus aureus (MRSA. The analysis included 84,843 patient admissions between January 2006 and December 2011 from 81 ICUs. Stratified Cox models were used to study multiple time scales while accounting for spatial clustering of the data (patients within ICUs and for death or discharge as competing events for MRSA infection. Results Both time scales, time in ICU and calendar time, are highly associated with the MRSA hazard rate and cumulative risk. When using only one basic time scale, the interpretation and magnitude of several patient-individual risk factors differed. Risk factors concerning the severity of illness were more pronounced when using only calendar time. These differences disappeared when using both time scales simultaneously. Conclusions The time-dependent dynamics of infections is complex and should be studied with models allowing for multiple time scales. For patient individual risk-factors we recommend stratified Cox regression models for competing events with ICU time as the basic time scale and calendar time as a covariate. The inclusion of calendar time and stratification by ICU

  5. Characteristic time scales in the American dollar-Mexican peso exchange currency market (United States)

    Alvarez-Ramirez, Jose


    Daily fluctuations of the American dollar-Mexican peso exchange currency market are studied using multifractal analysis methods. It is found evidence of multiaffinity of daily fluctuations in the sense that the qth-order (roughness) Hurst exponent Hq varies with changes in q. It is also found that there exist several characteristic time scales ranging from week to year. Accordingly, the market exhibits persistence in the sense that instabilities introduced by market events acting around the characteristic time scales (mainly, quarter and year) would propagate through the future market activity. Some implications of our results on the regulation of the dollar-mexpeso market activity are discussed.

  6. Doubly stochastic Poisson process models for precipitation at fine time-scales (United States)

    Ramesh, Nadarajah I.; Onof, Christian; Xie, Dichao


    This paper considers a class of stochastic point process models, based on doubly stochastic Poisson processes, in the modelling of rainfall. We examine the application of this class of models, a neglected alternative to the widely-known Poisson cluster models, in the analysis of fine time-scale rainfall intensity. These models are mainly used to analyse tipping-bucket raingauge data from a single site but an extension to multiple sites is illustrated which reveals the potential of this class of models to study the temporal and spatial variability of precipitation at fine time-scales.

  7. On Qualitative Analysis of Delay Systems and $x^ = f (t, x, x^)$ on Time Scales

    Indian Academy of Sciences (India)

    Yajun Ma; Yu Zhang; Jitao Sun


    Here we solve two problems presented in paper [9] (C C Tisdell and A Zaidi, Basic qualitative and quantitative results for solutions to nonlinear, dynamic equations on time scales with an application to economic modelling, Nonlinear Anal. 68 (2008) 3504–3524). We study existence and uniqueness of solutions for delay systems and first-order dynamic equations of the form $x^=f(t,x,x^)$ on time scales by using the Banach’s fixed-point theorem. Some examples are presented to illustrate the efficiency of the proposed results.

  8. Application of Time Scale to Parameters Tuning of Active Disturbance Rejection Controller for Induction Motor

    Institute of Scientific and Technical Information of China (English)

    SHAO Li-wei; LIAO Xiao-zhong; ZHANG Yu-he


    Active disturbance rejection controller (ADRC) has good performance in induction motor (IM) control system, but controller parameter is difficult to tune. A method of tuning ADRC parameter by time scale is analyzed. The IM time scale is obtained by theoretical analysis. Combining the relations between scale time and ADRC parameters, ADRC parameter tuning in IM vector control based stator flux oriented is obtained. This parameter tuning method is validated by simulations and it provides a new technique for tuning of ADRC parameters of IM.

  9. Oscillation Criteria for Second-Order Quasilinear Neutral Delay Dynamic Equations on Time Scales

    Directory of Open Access Journals (Sweden)

    Guangrong Zhang


    Full Text Available We establish some new oscillation criteria for the second-order quasilinear neutral delay dynamic equations [r(t(zΔ(tγ]Δ+q1(txα(τ1(t+q2(txβ(τ2(t=0 on a time scale 𝕋, where z(t=x(t+p(tx(τ0(t, 0<α<γ<β. Our results generalize and improve some known results for oscillation of second-order nonlinear delay dynamic equations on time scales. Some examples are considered to illustrate our main results.

  10. The Available Time Scale: Measuring Foster Parents' Available Time to Foster (United States)

    Cherry, Donna J.; Orme, John G.; Rhodes, Kathryn W.


    This article presents a new measure of available time specific to fostering, the Available Time Scale (ATS). It was tested with a national sample of 304 foster mothers and is designed to measure the amount of time foster parents are able to devote to fostering activities. The ATS has excellent reliability, and good support exists for its validity.…

  11. Design of turbulent tangential micro-mixers that mix liquids on the nanosecond time scale

    NARCIS (Netherlands)

    Mitic, Sandra; Nieuwkasteele, van Jan W.; Berg, van den Albert; Vries, de Simon


    Unravelling (bio)chemical reaction mechanisms and macromolecular folding pathways on the (sub)microsecond time scale is limited by the time resolution of kinetic instruments for mixing reactants and observation of the progress of the reaction. To improve the mixing time resolution, turbulent four- a

  12. Influence of the time scale on the construction of financial networks.

    Directory of Open Access Journals (Sweden)

    Frank Emmert-Streib

    Full Text Available BACKGROUND: In this paper we investigate the definition and formation of financial networks. Specifically, we study the influence of the time scale on their construction. METHODOLOGY/PRINCIPAL FINDINGS: For our analysis we use correlation-based networks obtained from the daily closing prices of stock market data. More precisely, we use the stocks that currently comprise the Dow Jones Industrial Average (DJIA and estimate financial networks where nodes correspond to stocks and edges correspond to none vanishing correlation coefficients. That means only if a correlation coefficient is statistically significant different from zero, we include an edge in the network. This construction procedure results in unweighted, undirected networks. By separating the time series of stock prices in non-overlapping intervals, we obtain one network per interval. The length of these intervals corresponds to the time scale of the data, whose influence on the construction of the networks will be studied in this paper. CONCLUSIONS/SIGNIFICANCE: Numerical analysis of four different measures in dependence on the time scale for the construction of networks allows us to gain insights about the intrinsic time scale of the stock market with respect to a meaningful graph-theoretical analysis.

  13. Existence of Solutions for Nonlinear Four-Point -Laplacian Boundary Value Problems on Time Scales

    Directory of Open Access Journals (Sweden)

    Topal SGulsan


    Full Text Available We are concerned with proving the existence of positive solutions of a nonlinear second-order four-point boundary value problem with a -Laplacian operator on time scales. The proofs are based on the fixed point theorems concerning cones in a Banach space. Existence result for -Laplacian boundary value problem is also given by the monotone method.

  14. Mixing and flushing time scales in the Azhikode Estuary, southwest coast of India

    Digital Repository Service at National Institute of Oceanography (India)

    Revichandran, C.; Pylee, A.

    Flushing time scales of the Azhikode Estuary, Kerala, India showed pronounced dry season and wet season signals as well as large inter-annual variation. Cumulative flushing time of the estuary varies from 4.8 tide cycles in April to 1.22 tide cycles...


    Institute of Scientific and Technical Information of China (English)


    Through the use of generalized Riccati transformation techniques, we establish some oscillation criteria for one type of nonlinear dynamic equation on time scales. Several examples, including a semilinear dynamic equation and a nonlinear Emden-Fowler dynamic equation, are also given to illustrate these criteria and to improve the results obtained in some references.

  16. Mass action realizations of reaction kinetic system models on various time scales

    Energy Technology Data Exchange (ETDEWEB)

    Hangos, K M; Szederkenyi, G, E-mail:, E-mail: [Process Control Research Group, Computer and Automation Reseach Institute, Kende u. 13-17, H-1111 Budapest (Hungary)


    Complex chemical reaction networks often exhibit different dynamic behaviour on different time scales. A combined approach is proposed in this work for determining physically meaningful mass action realizations of complex chemical reaction networks that describe its dynamic behaviour on different time scales. This is achieved by appropriately reducing the detailed overall mass action kinetic scheme using quasi steady state assumptions fit to the particular time scale, and then searching for an optimal realization using mixed integer linear programing. Furthermore, the relationship between the properties (reversibility, deficiency, stability) of the obtained realizations of the same system on different time scales are also investigated and related to the same properties of the detailed overall model. It is shown that the reduced models obtained by quasi steady state assumptions may show exotic nonlinear behaviour, such as oscillations, when the original detailed is globally asymptotically stable. The proposed methods are illustrated by using a simple Michaelis-Menten type reaction kinetic example. The simplified versions of the well known Brusselator model have also been investigated and presented as a case study.

  17. A limit set trichotomy for order-preserving systems on time scales

    Directory of Open Access Journals (Sweden)

    Christian Poetzsche


    Full Text Available In this paper we derive a limit set trichotomy for abstract order-preserving 2-parameter semiflows in normal cones of strongly ordered Banach spaces. Additionally, to provide an example, Muller's theorem is generalized to dynamic equations on arbitrary time scales and applied to a model from population dynamics.


    Institute of Scientific and Technical Information of China (English)

    Liu Yang; Chunfang Shen


    By a fixed point theorem in a cone,the existence of at least three positive solutions to a class of second-order multi-point boundary value problem for dynamic equation on time scales with the nonlinear term depends on the first order derivative is studied.

  19. Fixation of competing strategies when interacting agents differ in the time scale of strategy updating (United States)

    Zhang, Jianlei; Weissing, Franz J.; Cao, Ming


    A commonly used assumption in evolutionary game theory is that natural selection acts on individuals in the same time scale; e.g., players use the same frequency to update their strategies. Variation in learning rates within populations suggests that evolutionary game theory may not necessarily be restricted to uniform time scales associated with the game interaction and strategy adaption evolution. In this study, we remove this restricting assumption by dividing the population into fast and slow groups according to the players' strategy updating frequencies and investigate how different strategy compositions of one group influence the evolutionary outcome of the other's fixation probabilities of strategies within its own group. Analytical analysis and numerical calculations are performed to study the evolutionary dynamics of strategies in typical classes of two-player games (prisoner's dilemma game, snowdrift game, and stag-hunt game). The introduction of the heterogeneity in strategy-update time scales leads to substantial changes in the evolution dynamics of strategies. We provide an approximation formula for the fixation probability of mutant types in finite populations and study the outcome of strategy evolution under the weak selection. We find that although heterogeneity in time scales makes the collective evolutionary dynamics more complicated, the possible long-run evolutionary outcome can be effectively predicted under technical assumptions when knowing the population composition and payoff parameters.

  20. Time scales: from Nabla calculus to Delta calculus and vice versa via duality


    Caputo, M. Cristina


    In this note we show how one can obtain results from the nabla calculus from results on the delta calculus and vice versa via a duality argument. We provide applications of the main results to the calculus of variations on time scales.


    Institute of Scientific and Technical Information of China (English)

    Emin (O)zyilmaz


    The general idea in this paper is to study curves of the parametric equations where the parameter varies in a so-called time scale, which may be an arbitrary closed subset of the set of all real numbers. We introduce the directional derivative according to the vector fields.

  2. Stability and Convergence of Solutions to Volterra Integral Equations on Time Scales

    Directory of Open Access Journals (Sweden)

    Eleonora Messina


    Full Text Available We consider Volterra integral equations on time scales and present our study about the long time behavior of their solutions. We provide sufficient conditions for the stability and investigate the convergence properties when the kernel of the equations vanishes at infinity.

  3. The time scales of the scattering of energetic protons in interplanetary space


    Stevens, G.; Hick, P


    Observations with the directional spectrometer DFH aboard ISEE3 have been used to obtain results on the scattering time scales of energetic protons. Depending on the duration of the scattering process the particle distribution will be subjected to either phase scattering or full scattering. Our analysis of some representative events shows that full scattering is applicable to shock-associated events.


    Institute of Scientific and Technical Information of China (English)

    Geng Fengjie; Zhu Deming; Li Hongzhi


    The three-point boundary value problems of p-Laplacian dynamic equations on time scales are investigated. By using Krasnosel'skii's fixed-point theorem and fixed-point index theorem, criteria are achieved for the existence of at least one, two or 2n positive solutions.Furthermore, some examples are included to illustrate the main theorems.

  5. Fission time-scale from the measurement of pre-scission light particles and -ray multiplicities

    Indian Academy of Sciences (India)

    K Ramachandran; A Chatterjee; A Navin; K Mahata; A Shrivastava; V Tripathi; S Kailas; V Nanal; R G Pillay; A Saxena; R G Thomas; D R Chakrabarty; V M Datar; Suresh Kumar; P K Sahu


    An overview of the experimental result on simultaneous measurement of pre-scission neutron, proton, -particle and GDR -ray multiplicities for the reaction 28Si+175Lu at 159 MeV using the BARC–TIFR Pelletron–LINAC accelerator facility is given. The data were analysed using deformation-dependent particle transmission coefficients, binding energies and level densities which are incorporated in the code JOANNE2 to extract fission time-scales and mean deformation of the saddle-to-scission emitter. The neutron, light charged particle and GDR -ray multiplicity data could be explained consistently. The emission of neutrons seems to be favoured towards larger deformation as compared to charged particles. The pre-saddle time-scale is deduced as (0–2) × 10−21 s whereas the saddle-to-scission time-scale is (36–39) × 10−21 s. The total fission time-scale is deduced as (36–41) × 10−21 s.

  6. Hardy inequality on time scales and its application to half-linear dynamic equations

    Directory of Open Access Journals (Sweden)

    Řehák Pavel


    Full Text Available A time-scale version of the Hardy inequality is presented, which unifies and extends well-known Hardy inequalities in the continuous and in the discrete setting. An application in the oscillation theory of half-linear dynamic equations is given.


    Institute of Scientific and Technical Information of China (English)


    We investigate the type of singularity and qualitative structure of solutions to a time-invariant linear dynamic system on time scales. The results truly unify the qualitative behaviors of the system on the continuous and discrete times with any step size.


    Institute of Scientific and Technical Information of China (English)

    Yepeng Xing; Qiong Wang; Valery G. Romanovski


    We prove several new comparison results and develop the monotone iterative tech-nique to show the existence of extremal solutions to a kind of periodic boundary value problem (PBVP) for nonlinear integro-differential equation of mixed type on time scales.

  9. Multi-time Scale Coordination of Distributed Energy Resources in Isolated Power Systems

    Energy Technology Data Exchange (ETDEWEB)

    Mayhorn, Ebony; Xie, Le; Butler-Purry, Karen


    In isolated power systems, including microgrids, distributed assets, such as renewable energy resources (e.g. wind, solar) and energy storage, can be actively coordinated to reduce dependency on fossil fuel generation. The key challenge of such coordination arises from significant uncertainty and variability occurring at small time scales associated with increased penetration of renewables. Specifically, the problem is with ensuring economic and efficient utilization of DERs, while also meeting operational objectives such as adequate frequency performance. One possible solution is to reduce the time step at which tertiary controls are implemented and to ensure feedback and look-ahead capability are incorporated to handle variability and uncertainty. However, reducing the time step of tertiary controls necessitates investigating time-scale coupling with primary controls so as not to exacerbate system stability issues. In this paper, an optimal coordination (OC) strategy, which considers multiple time-scales, is proposed for isolated microgrid systems with a mix of DERs. This coordination strategy is based on an online moving horizon optimization approach. The effectiveness of the strategy was evaluated in terms of economics, technical performance, and computation time by varying key parameters that significantly impact performance. The illustrative example with realistic scenarios on a simulated isolated microgrid test system suggests that the proposed approach is generalizable towards designing multi-time scale optimal coordination strategies for isolated power systems.


    Institute of Scientific and Technical Information of China (English)


    In this paper,we investigate a second order impulsive differential equation on time scales.Sufficient conditions are given to guarantee that the solutions tend to zero.The notable effect of impulse upon the asymptotic behavior of solutions is stressed in this paper.At last,we illustrate our results with two examples.

  11. Periodic Solutions for a Class of Singular Hamiltonian Systems on Time Scales

    Directory of Open Access Journals (Sweden)

    Xiaofang Meng


    Full Text Available We are concerned with a class of singular Hamiltonian systems on time scales. Some results on the existence of periodic solutions are obtained for the system under consideration by means of the variational methods and the critical point theory.

  12. Time scale of scour around a pile in combined waves and current

    DEFF Research Database (Denmark)

    Petersen, Thor Ugelvig; Sumer, B. Mutlu; Fredsøe, Jørgen

    The time scale of the scour process around a circular vertical pile is studied in combined waves and current. A series of tests were carried out in a flume with pile diameters 40 mm and 75 mm, in both steady current, waves and combined waves and current. In the combined wave and current flow regime...

  13. A multiple time-scale model for TCP bandwidth sharing under user heterogeneity

    NARCIS (Netherlands)

    Abendroth, D.; Berg, J.L. van den; Mandjes, M.


    Building on the vast body of existing TCP models, we develop a novel versatile model that explicitly captures user heterogeneity, and takes into consideration dynamics at both the packet level and the flow level. It is described how the resulting multiple time-scale model can be numerically evaluate

  14. Arctic energy budget in relation to sea ice variability on monthly-to-annual time scales

    NARCIS (Netherlands)

    Krikken, F.; Hazeleger, W.


    The large decrease in Arctic sea ice in recent years has triggered a strong interest in Arctic sea ice predictions on seasonal-to-decadal time scales. Hence, it is important to understand physical processes that provide enhanced predictability beyond persistence of sea ice anomalies. This study anal

  15. Almost Periodic Solutions to Dynamic Equations on Time Scales and Applications

    Directory of Open Access Journals (Sweden)

    Yongkun Li


    equations on time scales. Then, as an application, using these concepts and results, we establish sufficient conditions for the existence and exponential stability of almost periodic solution to a class of Hopfield neural networks with delays. Finally, two examples and numerical simulations given to illustrate our results are plausible and meaningful.

  16. Charting the Transient Radio Sky on Sub-Second Time-Scales with LOFAR

    NARCIS (Netherlands)

    Hessels, J.W.T.


    The LOw Frequency ARray (LOFAR) is a radio interferometric telescope that promises to open a largely unexplored window on transient sources in the "radio sky", from time-scales of nanoseconds to years. An important aspect of this will be the study of radio-emitting neutron stars in their various inc

  17. Anti-control of chaos of single time-scale brushless DC motor. (United States)

    Ge, Zheng-Ming; Chang, Ching-Ming; Chen, Yen-Sheng


    Anti-control of chaos of single time-scale brushless DC motors is studied in this paper. In order to analyse a variety of periodic and chaotic phenomena, we employ several numerical techniques such as phase portraits, bifurcation diagrams and Lyapunov exponents. Anti-control of chaos can be achieved by adding an external constant term or an external periodic term.

  18. Assessment of the methods for determining net radiation at different time-scales of meteorological variables

    Directory of Open Access Journals (Sweden)

    Ni An


    Full Text Available When modeling the soil/atmosphere interaction, it is of paramount importance to determine the net radiation flux. There are two common calculation methods for this purpose. Method 1 relies on use of air temperature, while Method 2 relies on use of both air and soil temperatures. Nowadays, there has been no consensus on the application of these two methods. In this study, the half-hourly data of solar radiation recorded at an experimental embankment are used to calculate the net radiation and long-wave radiation at different time-scales (half-hourly, hourly, and daily using the two methods. The results show that, compared with Method 2 which has been widely adopted in agronomical, geotechnical and geo-environmental applications, Method 1 is more feasible for its simplicity and accuracy at shorter time-scale. Moreover, in case of longer time-scale, daily for instance, less variations of net radiation and long-wave radiation are obtained, suggesting that no detailed soil temperature variations can be obtained. In other words, shorter time-scales are preferred in determining net radiation flux.

  19. Existence and global exponential stability of periodic solutions for n-dimensional neutral dynamic equations on time scales. (United States)

    Li, Bing; Li, Yongkun; Zhang, Xuemei


    In this paper, by using the existence of the exponential dichotomy of linear dynamic equations on time scales and the theory of calculus on time scales, we study the existence and global exponential stability of periodic solutions for a class of n-dimensional neutral dynamic equations on time scales. We also present an example to illustrate the feasibility of our results. The results of this paper are completely new and complementary to the previously known results even in both the case of differential equations (time scale [Formula: see text]) and the case of difference equations (time scale [Formula: see text]).

  20. A hybrid procedure for MSW generation forecasting at multiple time scales in Xiamen City, China

    Energy Technology Data Exchange (ETDEWEB)

    Xu, Lilai, E-mail: [Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021 (China); Xiamen Key Lab of Urban Metabolism, Xiamen 361021 (China); Gao, Peiqing, E-mail: [Xiamen City Appearance and Environmental Sanitation Management Office, 51 Hexiangxi Road, Xiamen 361004 (China); Cui, Shenghui, E-mail: [Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021 (China); Xiamen Key Lab of Urban Metabolism, Xiamen 361021 (China); Liu, Chun, E-mail: [Xiamen City Appearance and Environmental Sanitation Management Office, 51 Hexiangxi Road, Xiamen 361004 (China)


    Highlights: ► We propose a hybrid model that combines seasonal SARIMA model and grey system theory. ► The model is robust at multiple time scales with the anticipated accuracy. ► At month-scale, the SARIMA model shows good representation for monthly MSW generation. ► At medium-term time scale, grey relational analysis could yield the MSW generation. ► At long-term time scale, GM (1, 1) provides a basic scenario of MSW generation. - Abstract: Accurate forecasting of municipal solid waste (MSW) generation is crucial and fundamental for the planning, operation and optimization of any MSW management system. Comprehensive information on waste generation for month-scale, medium-term and long-term time scales is especially needed, considering the necessity of MSW management upgrade facing many developing countries. Several existing models are available but of little use in forecasting MSW generation at multiple time scales. The goal of this study is to propose a hybrid model that combines the seasonal autoregressive integrated moving average (SARIMA) model and grey system theory to forecast MSW generation at multiple time scales without needing to consider other variables such as demographics and socioeconomic factors. To demonstrate its applicability, a case study of Xiamen City, China was performed. Results show that the model is robust enough to fit and forecast seasonal and annual dynamics of MSW generation at month-scale, medium- and long-term time scales with the desired accuracy. In the month-scale, MSW generation in Xiamen City will peak at 132.2 thousand tonnes in July 2015 – 1.5 times the volume in July 2010. In the medium term, annual MSW generation will increase to 1518.1 thousand tonnes by 2015 at an average growth rate of 10%. In the long term, a large volume of MSW will be output annually and will increase to 2486.3 thousand tonnes by 2020 – 2.5 times the value for 2010. The hybrid model proposed in this paper can enable decision makers to

  1. Diagonal chromatography to study plant protein modifications. (United States)

    Walton, Alan; Tsiatsiani, Liana; Jacques, Silke; Stes, Elisabeth; Messens, Joris; Van Breusegem, Frank; Goormachtig, Sofie; Gevaert, Kris


    An interesting asset of diagonal chromatography, which we have introduced for contemporary proteome research, is its high versatility concerning proteomic applications. Indeed, the peptide modification or sorting step that is required between consecutive peptide separations can easily be altered and thereby allows for the enrichment of specific, though different types of peptides. Here, we focus on the application of diagonal chromatography for the study of modifications of plant proteins. In particular, we show how diagonal chromatography allows for studying proteins processed by proteases, protein ubiquitination, and the oxidation of protein-bound methionines. We discuss the actual sorting steps needed for each of these applications and the obtained results. This article is part of a Special Issue entitled: Plant Proteomics--a bridge between fundamental processes and crop production, edited by Dr. Hans-Peter Mock.

  2. Time scales of autonomic information flow in near-term fetal sheep

    Directory of Open Access Journals (Sweden)

    Martin eFrasch


    Full Text Available Autonomic information flow (AIF characterizes fetal heart rate (FHR variability (fHRV in the time scale dependent complexity domain and discriminates sleep states (high voltage/low frequency (HV/LF and low voltage/high frequency (LV/HF electrocortical activity. However, the physiologic relationship of AIF time scales to the underlying sympathetic and vagal rhythms is not known. Understanding this relationship will enhance the benefits derived from using fHRV to monitor fetal health non-invasively. We analyzed AIF measured as Kullback-Leibler entropy in fetal sheep in late gestation as function of vagal and sympathetic modulation of fHRV, using atropine and propranolol respectively (n=6, and also analyzed changes in fHRV during sleep states (n=12. Atropine blockade resulted in complexity decrease at 2.5 Hz compared to baseline HV/LF and LV/HF states and at 1.6 Hz compared to LV/HF. Propranolol blockade resulted in complexity increase in the 0.8-1 Hz range compared to LV/HF and in no changes when compared to HV/LF. During LV/HF state activity, fHRV complexity was lower at 2.5 Hz and higher at 0.15-0.19 Hz than during HV/LF. Our findings show that in mature fetuses near term vagal activity contributes to fHRV complexity on a wider range of time scales than sympathetic activity. Related to sleep, during LV/HF we found lower complexity at short-term time scale where complexity is also decreased due to vagal blockade. We conclude that vagal and sympathetic modulations of fHRV show sleep state-dependent and time scale-dependent complexity patterns captured by AIF analysis of fHRV. Specifically, we observed a vagally mediated and sleep state-dependent change in these patterns at a time scale around 2.5 Hz (0.2 seconds. A paradigm of state-dependent nonlinear sympathovagal modulation of fHRV is discussed.

  3. Orthogonal separation and identification of long-chain peptides from scorpion Buthus martensi Karsch venom by using two-dimensional mixed-mode reversed phase-reversed phase chromatography coupled to tandem mass spectrometry. (United States)

    Xu, Junyan; Zhang, Xiuli; Guo, Zhimou; Yan, Jingyu; Yu, Long; Li, Xiuling; Xue, Xingya; Liang, Xinmiao


    Peptide components of scorpion venom have been employed as useful pharmacological tools in the study of ion channel function. The isolation of individual components is necessary for determination of their biological significance. Here, we have described a novel reversed phase (RP)/ion exchange stationary phase, Click oligo ethylene glycol (Click OEG), and the chromatographic efficiency of its mixed-mode sorbent in peptide separation experiments. The Click OEG presents a mixed-mode RP/weak anion-exchange type stationary phase at pH 3.5 and mixed-mode RP/weak cation-exchange type stationary phase at pH 6.0, and it was suitable for separation of long-chain peptides in scorpion venom. Subsequently, a two dimensional mixed-mode RP-RP system based Click OEG and C18 with different pH values in two dimensions was developed for orthogonal separation of scorpion venom. Furthermore, two fractions were analyzed in depth, and 11 long-chain peptides were purified and sequences were identified by using tandem mass spectrometry incorporating the tryptic approach. Among these, we isolated six novel peptides including one peptide with a new sequence and five transcript-level peptides, and speculated on their possible bioactivities.

  4. Small-time scale network traffic prediction based on a local support vector machine regression model

    Institute of Scientific and Technical Information of China (English)

    Meng Qing-Fang; Chen Yue-Hui; Peng Yu-Hua


    In this paper we apply the nonlinear time series analysis method to small-time scale traffic measurement data. The prediction-based method is used to determine the embedding dimension of the traffic data. Based on the reconstructed phase space, the local support vector machine prediction method is used to predict the traffic measurement data, and the BIC-based neighbouring point selection method is used to choose the number of the nearest neighbouring points for the local support vector machine regression model. The experimental results show that the local support vector machine prediction method whose neighbouring points are optimized can effectively predict the small-time scale traffic measurement data and can reproduce the statistical features of real traffic measurements.

  5. Time-Scale and Noise Optimality in Self-Organized Critical Adaptive Networks

    CERN Document Server

    Kuehn, Christian


    Recent studies have shown that adaptive networks driven by simple local rules can organize into "critical" global steady states, thereby providing another framework for self-organized criticality (SOC). Here we study SOC in an adaptive network considered first by Bornholdt and Rohlf [PRL, 84(26), p.6114-6117, 2000]. We focus on the important convergence to criticality and discover time-scale and noise optimal behaviour as well as a noise-induced phase transition. Due to the complexity of adaptive networks dynamics we suggest to investigate each effect separately by developing simple models. These models reveal three generically possible low-dimensional dynamical behaviors: time-scale resonance (TR), a simplified version of stochastic resonance - which call steady state stochastic resonance (SSR) - as well as noise-induced phase transitions. Thereby, our study not only opens up new directions for optimality in SOC but also applies to a much wider class of dynamical systems.

  6. Selection of outputs for distributed parameter systems by identifiability analysis in the time-scale domain (United States)

    Teergele, Jane; Danai, Kourosh


    A method of sensor location selection is introduced for distributed parameter systems. In this method, the sensitivities of spatial outputs to model parameters are computed by a model and transformed via continuous wavelet transforms into the time-scale domain to characterise the shape attributes of output sensitivities and accentuate their differences. Regions are then sought in the time-scale plane wherein the wavelet coefficient of an output sensitivity surpasses all the others' as indication of the output sensitivity's distinctness. This yields a comprehensive account of identifiability each output provides to the model parameters as the basis of output selection. The proposed output selection strategy is demonstrated for a numerical case of pollutant dispersion by advection and diffusion in a two-dimensional area.

  7. Stability analysis of nonlinear systems by multiple time scaling. [using perturbation methods (United States)

    Morino, L.


    The asymptotic solution for the transient analysis of a general nonlinear system in the neighborhood of the stability boundary was obtained by using the multiple-time-scaling asymptotic-expansion method. The nonlinearities are assumed to be of algebraic nature. Terms of order epsilon to the 3rd power (where epsilon is the order of amplitude of the unknown) are included in the solution. The solution indicates that there is always a limit cycle which is stable (unstable) and exists above (below) the stability boundary if the nonlinear terms are stabilizing (destabilizing). Extension of the solution to include fifth order nonlinear terms is also presented. Comparisons with harmonic balance and with multiple-time-scaling solution of panel flutter equations are also included.

  8. Characteristic time scales of mixing, mass transfer and biomass growth in a Taylor vortex algal photobioreactor. (United States)

    Gao, Xi; Kong, Bo; Vigil, R Dennis


    Recently it has been demonstrated that algal biomass yield can be enhanced using fluid flow patterns known as Taylor vortices. It has been suggested that these growth rate improvements can be attributed to improved light delivery as a result of rapid transport of microorganisms between light and dark regions of the reactor. However, Taylor vortices also strongly impact fluid mixing and interphase (gas-liquid) mass transport, and these in turn may also explain improvements in biomass productivity. To identify the growth-limiting factor in a Taylor vortex algal photobioreactor, experiments were performed to determine characteristic time scales for mixing and mass transfer. By comparing these results with the characteristic time scale for biomass growth, it is shown that algal growth rate in Taylor vortex reactors is not limited by fluid mixing or interphase mass transfer, and therefore the observed biomass productivity improvements are likely attributable to improved light utilization efficiency.

  9. Time-scale effects on the gain-loss asymmetry in stock indices

    CERN Document Server

    Sándor, Bulcsú


    The gain-loss asymmetry, observed in the inverse statistics of stock indices is present for logarithmic return levels that are over $2\\%$, and it is the result of the non-Pearson type auto-correlations in the index. These non-Pearson type correlations can be viewed also as functionally dependent daily volatilities, extending for a finite time interval. A generalized time-window shuffling method is used to show the existence of such auto-correlations. Their characteristic time-scale proves to be smaller (less than $25$ trading days) than what was previously believed. It is also found that this characteristic time-scale has decreased with the appearance of program trading in the stock market transactions. Connections with the leverage effect are also established.

  10. Time-scale effects on the gain-loss asymmetry in stock indices (United States)

    Sándor, Bulcsú; Simonsen, Ingve; Nagy, Bálint Zsolt; Néda, Zoltán


    The gain-loss asymmetry, observed in the inverse statistics of stock indices is present for logarithmic return levels that are over 2 % , and it is the result of the non-Pearson-type autocorrelations in the index. These non-Pearson-type correlations can be viewed also as functionally dependent daily volatilities, extending for a finite time interval. A generalized time-window shuffling method is used to show the existence of such autocorrelations. Their characteristic time scale proves to be smaller (less than 25 trading days) than what was previously believed. It is also found that this characteristic time scale has decreased with the appearance of program trading in the stock market transactions. Connections with the leverage effect are also established.

  11. Climatic changes on millennial time scales--Evidence from a high-resolution loess record

    Institute of Scientific and Technical Information of China (English)

    任剑璋; 丁仲礼; 刘东生; 孙继敏; 周晓权


    Studies on a high resolution loess section in Huining County reveal that the behavior of climate shows high instability during the last glaciation. Results reflect that climate in Loess Plateau oscillates on millennial time scales during the last glacial period. These can be teleconnected with the records of Dansgaard-Oeschger cycles and Heinrich events in high latitudes. Results also demonstrate that variations in the intensity of wind regime on the Loess Plateau have a close correlation with the changes of global ice sheets volume. All these suggest that two-level fordngs may drive climate changes in central Asia. The first level is the volume changes of ice sheets and the second level with short time scales is superimposed upon the first level on a nearly global scale.

  12. A Visual Method of Time Scale Determination using a PC for Radio Sources

    Indian Academy of Sciences (India)

    Yong Huang; Jun-Hui Fan; Jing Pan


    Variability is one of the extremely observational properties. In the radio bands, variability is caused by the shock in the jet. In this case, emissions increase rapidly following an exponential curve, and then decrease rapidly also in an exponential curve. The variability time scale is important with regard to the physics carrying on in the jet. However, it is not easy to fit the light curve. In this paper, we proposed a method of light curve fitting on a PC machine, in which the theoretical exponential light curve is adopted to the observations using the least regression method. Using this method, anybody can fit the light curve and get the time scale by moving and clicking themouse.We also used this method to some light curves obtained from the archive and compared our results with those in the literature.

  13. Multiple dynamical time-scales in networks with hierarchically nested modular organization

    Indian Academy of Sciences (India)

    Sitabhra Sinha; Swarup Poria


    Many natural and engineered complex networks have intricate mesoscopic organization, e.g., the clustering of the constituent nodes into several communities or modules. Often, such modularity is manifested at several different hierarchical levels, where the clusters defined at one level appear as elementary entities at the next higher level. Using a simple model of a hierarchical modular network, we show that such a topological structure gives rise to characteristic time-scale separation between dynamics occurring at different levels of the hierarchy. This generalizes our earlier result for simple modular networks, where fast intramodular and slow intermodular processes were clearly distinguished. Investigating the process of synchronization of oscillators in a hierarchical modular network, we show the existence of as many distinct time-scales as there are hierarchical levels in the system. This suggests a possible functional role of such mesoscopic organization principle in natural systems, viz., in the dynamical separation of events occurring at different spatial scales.

  14. Multiple-Time-Scales Hierarchical Frequency Stability Control Strategy of Medium-Voltage Isolated Microgrid

    DEFF Research Database (Denmark)

    Zhao, Zhuoli; Yang, Ping; Guerrero, Josep M.


    In this paper, an islanded medium-voltage (MV) microgrid placed in Dongao Island is presented, which integrates renewable-energy-based distributed generations (DGs), energy storage system (ESS), and local loads. In an isolated microgrid without connection to the main grid to support the frequency....... In this way, dynamic stability control that cope with disturbances in short-time scale is implemented by microgrid central controller (MGCC) within Zone B and Zone C. Meanwhile, steady-state stability control to solve the peaks and valleys problem of loads and DGs in long-time scale is executed by microgrid...... energy management system (MEMS) within Zone A. Furthermore, based on the developed complete small-signal state-space model, sensitivity analysis of the eigenvalues is conducted in order to reveal the dynamic stability margin of the MV microgrid, and to identify the proper range of the control parameters...

  15. Self-assembling of zinc phthalocyanines on ZnO (1010) surface through multiple time scales. (United States)

    Melis, Claudio; Raiteri, Paolo; Colombo, Luciano; Mattoni, Alessandro


    We adopt a hierarchic combination of theoretical methods to study the assembling of zinc phthalocyanines (ZnPcs) on a ZnO (1010) surface through multiple time scales. Atomistic simulations, such as model potential molecular dynamics and metadynamics, are used to study the energetics and short time evolution (up to ∼100 ns) of small ZnPc aggregates. The stability and the lifetime of large clusters is then studied by means of an atomistically informed coarse-grained model using classical molecular dynamics. Finally, the macroscopic time scale clustering phenomenon is studied by Metropolis Monte Carlo algorithms as a function of temperature and surface coverage. We provide evidence that at room temperature the aggregation is likely to occur at sufficiently high coverage, and we characterize the nature, morphology, and lifetime of ZnPc's clusters. We identify the molecular stripes oriented along [010] crystallographic directions as the most energetically stable aggregates.

  16. Digital signal processing techniques for pitch shifting and time scaling of audio signals (United States)

    Buś, Szymon; Jedrzejewski, Konrad


    In this paper, we present the techniques used for modifying the spectral content (pitch shifting) and for changing the time duration (time scaling) of an audio signal. A short introduction gives a necessary background for understanding the discussed issues and contains explanations of the terms used in the paper. In subsequent sections we present three different techniques appropriate both for pitch shifting and for time scaling. These techniques use three different time-frequency representations of a signal, namely short-time Fourier transform (STFT), continuous wavelet transform (CWT) and constant-Q transform (CQT). The results of simulation studies devoted to comparison of the properties of these methods are presented and discussed in the paper.


    Institute of Scientific and Technical Information of China (English)

    Zhou Ying; Zhang Linghua


    This paper improves and presents an advanced method of the voice conversion system based on Gaussian Mixture Models (GMM) models by changing the time-scale of speech.The Speech Transformation and Representation using Adaptive Interpolation of weiGHTed spectrum (STRAIGHT) model is adopted to extract the spectrum features,and the GMM models are trained to generate the conversion function.The spectrum features of a source speech will be converted by the conversion function.The time-scale of speech is changed by extracting the converted features and adding to the spectrum.The conversion voice was evaluated by subjective and objective measurements.The results confirm that the transformed speech not only approximates the characteristics of the target speaker,but also more natural and more intelligible.

  18. Continent-scale global change attribution in European birds - combining annual and decadal time scales

    DEFF Research Database (Denmark)

    Jørgensen, Peter Søgaard; Böhning-Gaese, Katrin; Thorup, Kasper;


    Species attributes are commonly used to infer impacts of environmental change on multiyear species trends, e.g. decadal changes in population size. However, by themselves attributes are of limited value in global change attribution since they do not measure the changing environment. A broader...... foundation for attributing species responses to global change may be achieved by complementing an attributes-based approach by one estimating the relationship between repeated measures of organismal and environmental changes over short time scales. To assess the benefit of this multiscale perspective, we...... on or in the peak of the breeding season with the largest effect sizes observed in cooler parts of species' climatic ranges. Our results document the potential of combining time scales and integrating both species attributes and environmental variables for global change attribution. We suggest such an approach...

  19. Universality and extremal aging for dynamics of spin glasses on sub-exponential time scales

    CERN Document Server

    Arous, G Ben


    We consider Random Hopping Time (RHT) dynamics of the Sherrington - Kirkpatrick (SK) model and p-spin models of spin glasses. For any of these models and for any inverse temperature we prove that, on time scales that are sub-exponential in the dimension, the properly scaled clock process (time-change process) of the dynamics converges to an extremal process. Moreover, on these time scales, the system exhibits aging like behavior which we called extremal aging. In other words, the dynamics of these models ages as the random energy model (REM) does. Hence, by extension, this confirms Bouchaud's REM-like trap model as a universal aging mechanism for a wide range of systems which, for the first time, includes the SK model.

  20. Analysis of the endogenous peptide profile of milk: identification of 248 mainly casein-derived peptides. (United States)

    Baum, Florian; Fedorova, Maria; Ebner, Jennifer; Hoffmann, Ralf; Pischetsrieder, Monika


    Milk is an excellent source of bioactive peptides. However, the composition of the native milk peptidome has only been partially elucidated. The present study applied matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) directly or after prefractionation of the milk peptides by reverse-phase high-performance liquid chromatography (RP-HPLC) or OFFGEL fractionation for the comprehensive analysis of the peptide profile of raw milk. The peptide sequences were determined by MALDI-TOF/TOF or nano-ultra-performance liquid chromatography-nanoelectrospray ionization-LTQ-Orbitrap-MS. Direct MALDI-TOF-MS analysis led to the assignment of 57 peptides. Prefractionation by both complementary methods led to the assignment of another 191 peptides. Most peptides originate from α(S1)-casein, followed by β-casein, and α(S2)-casein. κ-Casein and whey proteins seem to play only a minor role as peptide precursors. The formation of many, but not all, peptides could be explained by the activity of the endogenous peptidases, plasmin or cathepsin D, B, and G. Database searches revealed the presence of 22 peptides with established physiological function, including those with angiotensin-converting-enzyme (ACE) inhibitory, immunomodulating, or antimicrobial activity.

  1. Eleven-year cyclicity of the sun on the 2000-year time scale (United States)

    Nagovitsyn, Yu. A.; Georgieva, K.; Osipova, A. A.; Kuleshova, A. I.


    The paper describes the first reconstruction of the annual values of the total area and magnetic flux of sunspots, as well as the Wolf index, on the 2000-year time scale. The procedure was performed by combining data on solar cyclicity amplitudes obtained from a decadal reconstruction (Usoskin et al., 2014) and data on its phases acquired from the numbers of auroras, which is contained in catalogs by Křivský and Silverman.

  2. A typology of time-scale mismatches and behavioral interventions to diagnose and solve conservation problems (United States)

    Wilson, Robyn S.; Hardisty, David J.; Epanchin-Niell, Rebecca S.; Runge, Michael C.; Cottingham, Kathryn L.; Urban, Dean L.; Maguire, Lynn A.; Hastings, Alan; Mumby, Peter J.; Peters, Debra P. C.


    Ecological systems often operate on time scales significantly longer or shorter than the time scales typical of human decision making, which causes substantial difficulty for conservation and management in socioecological systems. For example, invasive species may move faster than humans can diagnose problems and initiate solutions, and climate systems may exhibit long-term inertia and short-term fluctuations that obscure learning about the efficacy of management efforts in many ecological systems. We adopted a management-decision framework that distinguishes decision makers within public institutions from individual actors within the social system, calls attention to the ways socioecological systems respond to decision makers’ actions, and notes institutional learning that accrues from observing these responses. We used this framework, along with insights from bedeviling conservation problems, to create a typology that identifies problematic time-scale mismatches occurring between individual decision makers in public institutions and between individual actors in the social or ecological system. We also considered solutions that involve modifying human perception and behavior at the individual level as a means of resolving these problematic mismatches. The potential solutions are derived from the behavioral economics and psychology literature on temporal challenges in decision making, such as the human tendency to discount future outcomes at irrationally high rates. These solutions range from framing environmental decisions to enhance the salience of long-term consequences, to using structured decision processes that make time scales of actions and consequences more explicit, to structural solutions aimed at altering the consequences of short-sighted behavior to make it less appealing. Additional application of these tools and long-term evaluation measures that assess not just behavioral changes but also associated changes in ecological systems are needed.

  3. Existence of Positive Solutions for Higher Order Boundary Value Problem on Time Scales

    Institute of Scientific and Technical Information of China (English)



    In this paper,we investigate the existence of positive solutions of a class higher order boundary value problems on time scales.The class of boundary value problems educes a four-point (or three-point or two-point) boundary value problems,for which some similar results are established.Our approach relies on the Krasnosel'skii fixed point theorem.The result of this paper is new and extends previously known results.

  4. Multiplicity results for a generalized Sturm-Liouville dynamical system on time scales


    Zhang Youwei


    Abstract By applying the fixed point theorem in cones, some new and general results on the existence of positive solution to second order generalized Sturm-Liouville dynamical system on time scale T u 1 Δ Δ ( t ) + h 1 ( t ) f 1 ( t , u 1 ( t ) , u 2 ( t ) , u 1 Δ ( t ) , u 2 Δ ( t ) ) = 0 , u 2 Δ Δ ( t ) + h 2 ( t ) f 2 ( t , u 1 ( ...

  5. A time-scale analysis of systematic risk: wavelet-based approach


    Khalfaoui Rabeh, K; Boutahar Mohamed, B


    The paper studies the impact of different time-scales on the market risk of individual stock market returns and of a given portfolio in Paris Stock Market by applying the wavelet analysis. To investigate the scaling properties of stock market returns and the lead/lag relationship between them at different scales, wavelet variance and crosscorrelations analyses are used. According to wavelet variance, stock returns exhibit long memory dynamics. The wavelet cross-correlation analysis shows that...

  6. Lindstedt series and Hamilton-Jacobi equation for hyperbolic tori in three time scales problems

    CERN Document Server

    Gallavotti, G; Mastropietro, V; Gallavotti, Giovannni; Gentile, Guido; Astropietro, Vieri M


    Interacting systems consisting of two rotators and a pendulum are considered, in a case in which the uncoupled systems have three very different characteristic time scales. The abundance of unstable quasi periodic motions in phase space is studied via Lindstedt series. The result is a strong improvement, compared to our previous results, on the domain of validity of bounds that imply existence of invariant tori, large homoclinic angles, long heteroclinic chains and drift--diffusion in phase space.

  7. Lyapunov-type inequality for a higher order dynamic equation on time scales. (United States)

    Sun, Taixiang; Xi, Hongjian


    The purpose of this work is to establish a Lyapunov-type inequality for the following dynamic equation [Formula: see text]on some time scale T under the anti-periodic boundary conditions [Formula: see text], where [Formula: see text] for [Formula: see text] and [Formula: see text], [Formula: see text] with [Formula: see text] and [Formula: see text], p is the quotient of two odd positive integers and [Formula: see text] with [Formula: see text].

  8. Separation of time-scales and model reduction for stochastic reaction networks

    CERN Document Server

    Kang, Hye-Won


    A stochastic model for a chemical reaction network is embedded in a one-parameter family of models with species numbers and rate constants scaled by powers of the parameter. A systematic approach is developed for determining appropriate choices of the exponents that can be applied to large complex networks. When the scaling implies subnetworks have different time-scales, the subnetworks can be approximated separately providing insight into the behavior of the full network through the analysis of these lower dimensional approximations.

  9. Effects of moonlight on the capturability of frugivorous phyllostomid bats (Chiroptera: Phyllostomidae at different time scales

    Directory of Open Access Journals (Sweden)

    Marco A. R. Mello


    Full Text Available Some bat species seem to be lunar phobic, i.e., they avoid flying in bright areas or during bright periods of the night; however, the evidence is still controversial. We think that part of this controversy comes from pooling data on bat captures and moonlight intensity according to broad categories, such as moon phases, which conceal the high variability among nights. Therefore, we used detailed, long-term field data on three phyllostomid bat species, in order to test the hypothesis of lunar phobia at two different time scales: 1 among nights, by pooling data of different nights according to moon phases and testing for differences in the distribution of captures; and 2 within a night, by analyzing the relationship between capturability and moonlight intensity (measured as illuminance in one-hour intervals for 29 individual nights. Although most captures of the studied bat species occurred in the first half of the night, their activity pattern varied largely among nights, and was not always unimodal as commonly assumed. At the larger time scale, all studied bat species showed evidence of lunar phobia, as they were more frequently captured on dark moon phases. Nevertheless, at the smaller time scale, only Carollia perspicillata (Linnaeus, 1758 was less frequently captured on brighter periods of the night. We propose that the unimodal activity pattern assumed for frugivorous phyllostomid bats may be an artifact of data organization, and that activity and lunar phobia are much more variable than previously assumed.

  10. A biologically plausible model of time-scale invariant interval timing. (United States)

    Almeida, Rita; Ledberg, Anders


    The temporal durations between events often exert a strong influence over behavior. The details of this influence have been extensively characterized in behavioral experiments in different animal species. A remarkable feature of the data collected in these experiments is that they are often time-scale invariant. This means that response measurements obtained under intervals of different durations coincide when plotted as functions of relative time. Here we describe a biologically plausible model of an interval timing device and show that it is consistent with time-scale invariant behavior over a substantial range of interval durations. The model consists of a set of bistable units that switch from one state to the other at random times. We first use an abstract formulation of the model to derive exact expressions for some key quantities and to demonstrate time-scale invariance for any range of interval durations. We then show how the model could be implemented in the nervous system through a generic and biologically plausible mechanism. In particular, we show that any system that can display noise-driven transitions from one stable state to another can be used to implement the timing device. Our work demonstrates that a biologically plausible model can qualitatively account for a large body of data and thus provides a link between the biology and behavior of interval timing.

  11. A multi-time-scale analysis of chemical reaction networks: II. Stochastic systems. (United States)

    Kan, Xingye; Lee, Chang Hyeong; Othmer, Hans G


    We consider stochastic descriptions of chemical reaction networks in which there are both fast and slow reactions, and for which the time scales are widely separated. We develop a computational algorithm that produces the generator of the full chemical master equation for arbitrary systems, and show how to obtain a reduced equation that governs the evolution on the slow time scale. This is done by applying a state space decomposition to the full equation that leads to the reduced dynamics in terms of certain projections and the invariant distributions of the fast system. The rates or propensities of the reduced system are shown to be the rates of the slow reactions conditioned on the expectations of fast steps. We also show that the generator of the reduced system is a Markov generator, and we present an efficient stochastic simulation algorithm for the slow time scale dynamics. We illustrate the numerical accuracy of the approximation by simulating several examples. Graph-theoretic techniques are used throughout to describe the structure of the reaction network and the state-space transitions accessible under the dynamics.

  12. Measuring collective behaviour of multicellular ensembles: role of space–time scales

    Indian Academy of Sciences (India)

    S Rajesh; Somdatta Sinha


    Living systems are spectacular examples of spatiotemporally organized structures. During the development of complex organization there is dynamic equilibrium between the local and global processes acting at the intra- and intercellular levels in multiple space and time scales. Although in modelling studies such spatiotemporal systems can be described by different space–time scales and at many organizational levels, the experimental quantities measured and predictions useful for practical applications are at a macroscopic (coarser or averaged) level/scale; these are limited by the resolution of the measuring method and experimental protocol. In this work, we address whether the spatiotemporal collective dynamics exhibited by a multiscale system can discriminate between, or be borne out by, the coarse-grained and averaged measurements done at different spatial and temporal scales. Using a simple model of a ring of cells, we show that measurements of both spatial and spatiotemporal average behaviour in this multicellular ensemble can mask the variety of collective dynamics observed at other space–time scales, and exhibit completely different behaviours. Such outcomes of measurements can lead to incomplete and incorrect understanding of physiological functions and pathogenesis in multicell ensembles.

  13. Glottal closure instant and voice source analysis using time-scale lines of maximum amplitude

    Indian Academy of Sciences (India)

    Christophe D’Alessandro; Nicolas Sturmel


    1Time-scale representation of voiced speech is applied to voice quality analysis, by introducing the Line of Maximum Amplitude (LoMA) method. This representation takes advantage of the tree patterns observed for voiced speech periods in the time-scale domain. For each period, the optimal LoMA is computed by linking amplitude maxima at each scale of a wavelet transform, using a dynamic programming algorithm. A time-scale analysis of the linear acoustic model of speech production shows several interesting properties. The LoMA points to the glottal closure instants. The LoMA phase delay is linked to the voice open quotient. The cumulated amplitude along the LoMA is related to voicing amplitude. The LoMA spectral centre of gravity is an indication of voice spectral tilt. Following these theoretical considerations, experimental results are reported. Comparative evaluation demonstrates that the LoMA is an effective method for the detection of Glottal Closure Instants (GCI). The effectiveness of LoMA analysis for open quotient, amplitude and spectral tilt estimations is also discussed with the help of some examples.

  14. On the simulation of protein folding by short time scale molecular dynamics and distributed computing. (United States)

    Fersht, Alan R


    There are proposals to overcome the current incompatibilities between the time scales of protein folding and molecular dynamics simulation by using a large number of short simulations of only tens of nanoseconds (distributed computing). According to the principles of first-order kinetic processes, a sufficiently large number of short simulations will include, de facto, a small number of long time scale events that have proceeded to completion. But protein folding is not an elementary kinetic step: folding has a series of early conformational steps that lead to lag phases at the beginning of the kinetics. The presence of these lag phases can bias short simulations toward selecting minor pathways that have fewer or faster lag steps and so miss the major folding pathways. Attempts to circumvent the lags by using loosely coupled parallel simulations that search for first-order transitions are also problematic because of the difficulty of detecting transitions in molecular dynamics simulations. Nevertheless, the procedure of using parallel independent simulations is perfectly valid and quite feasible once the time scale of simulation proceeds past the lag phases into a single exponential region.

  15. A Black Hole Mass-Variability Time Scale Correlation at Submillimeter Wavelengths

    CERN Document Server

    Bower, Geoffrey C; Markoff, Sera; Gurwell, Mark A; Rao, Ramprasad; McHardy, Ian


    We analyze the light curves of 413 radio sources at submillimeter wavelengths using data from the Submillimeter Array calibrator database. The database includes more than 20,000 observations at 1.3 and 0.8 mm that span 13 years. We model the light curves as a damped random walk and determine a characteristic time scale $\\tau$ at which the variability amplitude saturates. For the vast majority of sources, primarily blazars and BL Lac objects, we find only lower limits on $\\tau$. For two nearby low luminosity active galactic nuclei, M81 and M87, however, we measure $\\tau=1.6^{+3.0}_{-0.9}$ days and $\\tau=45^{+61}_{-24}$ days, respectively ($2\\sigma$ errors). Including the previously measured $\\tau=0.33\\pm 0.16$ days for Sgr A*, we show an approximately linear correlation between $\\tau$ and black hole mass for these nearby LLAGN. Other LLAGN with spectra that peak in the submm are expected to follow this correlation. These characteristic time scales are comparable to the minimum time scale for emission processes...

  16. Modeling Climate Responses to Spectral Solar Forcing on Centennial and Decadal Time Scales (United States)

    Wen, G.; Cahalan, R.; Rind, D.; Jonas, J.; Pilewskie, P.; Harder, J.


    We report a series of experiments to explore clima responses to two types of solar spectral forcing on decadal and centennial time scales - one based on prior reconstructions, and another implied by recent observations from the SORCE (Solar Radiation and Climate Experiment) SIM (Spectral 1rradiance Monitor). We apply these forcings to the Goddard Institute for Space Studies (GISS) Global/Middle Atmosphere Model (GCMAM). that couples atmosphere with ocean, and has a model top near the mesopause, allowing us to examine the full response to the two solar forcing scenarios. We show different climate responses to the two solar forCing scenarios on decadal time scales and also trends on centennial time scales. Differences between solar maximum and solar minimum conditions are highlighted, including impacts of the time lagged reSponse of the lower atmosphere and ocean. This contrasts with studies that assume separate equilibrium conditions at solar maximum and minimum. We discuss model feedback mechanisms involved in the solar forced climate variations.

  17. Active open boundary forcing using dual relaxation time-scales in downscaled ocean models (United States)

    Herzfeld, M.; Gillibrand, P. A.


    Regional models actively forced with data from larger scale models at their open boundaries often contain motion at different time-scales (e.g. tidal and low frequency). These motions are not always individually well specified in the forcing data, and one may require a more active boundary forcing while the other exert less influence on the model interior. If a single relaxation time-scale is used to relax toward these data in the boundary equation, then this may be difficult. The method of fractional steps is used to introduce dual relaxation time-scales in an open boundary local flux adjustment scheme. This allows tidal and low frequency oscillations to be relaxed independently, resulting in a better overall solution than if a single relaxation parameter is optimized for tidal (short relaxation) or low frequency (long relaxation) boundary forcing. The dual method is compared to the single relaxation method for an idealized test case where a tidal signal is superimposed on a steady state low frequency solution, and a real application where the low frequency boundary forcing component is derived from a global circulation model for a region extending over the whole Great Barrier Reef, and a tidal signal subsequently superimposed.

  18. High pH reversed-phase chromatography with fraction concatenation as an alternative to strong-cation exchange chromatography for two-dimensional proteomic analysis


    Yang, Feng; Shen, Yufeng; Camp, David G.; Smith, Richard D.


    Orthogonal high-resolution separations are critical for attaining improved analytical dynamic range and protein coverage in proteomic measurements. High pH reversed-phase liquid chromatography (RPLC) followed by fraction concatenation affords better peptide analysis than conventional strong-cation exchange (SCX) chromatography applied for the two-dimensional proteomic analysis. For example, concatenated high pH reversed-phase liquid chromatography increased identification for peptides (1.8-fo...

  19. Earth History databases and visualization - the TimeScale Creator system (United States)

    Ogg, James; Lugowski, Adam; Gradstein, Felix


    The "TimeScale Creator" team ( and the Subcommission on Stratigraphic Information ( of the International Commission on Stratigraphy ( has worked with numerous geoscientists and geological surveys to prepare reference datasets for global and regional stratigraphy. All events are currently calibrated to Geologic Time Scale 2004 (Gradstein et al., 2004, Cambridge Univ. Press) and Concise Geologic Time Scale (Ogg et al., 2008, Cambridge Univ. Press); but the array of intercalibrations enable dynamic adjustment to future numerical age scales and interpolation methods. The main "global" database contains over 25,000 events/zones from paleontology, geomagnetics, sea-level and sequence stratigraphy, igneous provinces, bolide impacts, plus several stable isotope curves and image sets. Several regional datasets are provided in conjunction with geological surveys, with numerical ages interpolated using a similar flexible inter-calibration procedure. For example, a joint program with Geoscience Australia has compiled an extensive Australian regional biostratigraphy and a full array of basin lithologic columns with each formation linked to public lexicons of all Proterozoic through Phanerozoic basins - nearly 500 columns of over 9,000 data lines plus hot-curser links to oil-gas reference wells. Other datapacks include New Zealand biostratigraphy and basin transects (ca. 200 columns), Russian biostratigraphy, British Isles regional stratigraphy, Gulf of Mexico biostratigraphy and lithostratigraphy, high-resolution Neogene stable isotope curves and ice-core data, human cultural episodes, and Circum-Arctic stratigraphy sets. The growing library of datasets is designed for viewing and chart-making in the free "TimeScale Creator" JAVA package. This visualization system produces a screen display of the user-selected time-span and the selected columns of geologic time scale information. The user can change the

  20. An antifungal peptide from the coconut. (United States)

    Wang, H X; Ng, T B


    A chromatographic procedure consisting of ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, and gel filtration by fast performance liquid chromatography on Supedex 75 was utilized to isolate a 10 kDa antifungal peptide from coconut flesh. The peptide was unadsorbed on DEAE-cellulose, but adsorbed on Affi-gel blue gel and CM-cellulose. It displayed antifungal activity against Fusarium oxysporum, Mycosphaerella arachidicola and Physalospora piricola. The IC50 values of its inhibitory activities on mycelial growth in M. arachidicola and HIV-1 reverse transcriptase activity were respectively 1.2 and 52.5 microM.

  1. Screening of TACE Peptide Inhibitors from Phage Display Peptide Library

    Institute of Scientific and Technical Information of China (English)


    To obtain the recombinant tumor necrosis factor-α converting enzyme (TACE) ectodomain and use it as a selective molecule for the screening of TACE peptide inhibitors, the cDNA coding catalytic domain (T800) and full-length ectodomain (T1300) of TACE were amplified by RTPCR, and the expression plasmids were constructed by inserting T800 and T1300 into plasmid pET28a and pET-28c respectively. The recombinant T800 and T1300 were induced by IPTG, and SDSPAGE and Western blotting analysis results revealed that T800 and T1300 were highly expressed in the form of inclusion body. After Ni2+-NTA resin affinity chromatography, the recombinant proteins were used in the screening of TACE-binding peptides from phage display peptide library respectively. After 4 rounds of biopanning, the positive phage clones were analyzed by ELISA, competitive inhibition assay and DNA sequencing. A common amino acid sequence (TRWLVYFSRPYLVAT) was found and synthesized. The synthetic peptide could inhibit the TNF-α release from LPS-stimulated human peripheral blood mononuclear cells (PBMC) up to 60.3 %. FACS analysis revealed that the peptide mediated the accumulation of TNF-α on the cell surface. These results demonstrate that the TACE-binding peptide is an effective antagonist of TACE.

  2. Gas chromatography (United States)

    Guiochon, Georges; Guillemin, Claude L.


    Gas chromatography is a powerful separation technique for gas and vapor mixtures. Combining separation and on-line detection permits accurate quantitative analysis of complex mixtures, including traces of compounds down to parts per trillions in some particular cases. The importance of gas chromatography in quality control and process control in the chemical and drug industry, in environmental pollution investigations and in clinical analysis is critical. The principles of the technique are discussed, the main components of a gas chromatograph are described and some idea of the importance of the applications is given.

  3. Peptide identification (United States)

    Jarman, Kristin H [Richland, WA; Cannon, William R [Richland, WA; Jarman, Kenneth D [Richland, WA; Heredia-Langner, Alejandro [Richland, WA


    Peptides are identified from a list of candidates using collision-induced dissociation tandem mass spectrometry data. A probabilistic model for the occurrence of spectral peaks corresponding to frequently observed partial peptide fragment ions is applied. As part of the identification procedure, a probability score is produced that indicates the likelihood of any given candidate being the correct match. The statistical significance of the score is known without necessarily having reference to the actual identity of the peptide. In one form of the invention, a genetic algorithm is applied to candidate peptides using an objective function that takes into account the number of shifted peaks appearing in the candidate spectrum relative to the test spectrum.

  4. Intramolecular stable isotope distributions detect plant metabolic responses on century time scales (United States)

    Schleucher, Jürgen; Ehlers, Ina; Augusti, Angela; Betson, Tatiana


    Plants respond to environmental changes on a vast range of time scales, and plant gas exchanges constitute important feedback mechanisms in the global C cycle. Responses on time scales of decades to centuries are most important for climate models, for prediction of crop productivity, and for adaptation to climate change. Unfortunately, responses on these timescale are least understood. We argue that the knowledge gap on intermediate time scales is due to a lack of adequate methods that can bridge between short-term manipulative experiments (e.g. FACE) and paleo research. Manipulative experiments in plant ecophysiology give information on metabolism on time scales up to years. However, this information cannot be linked to results from retrospective studies in paleo research, because little metabolic information can be derived from paleo archives. Stable isotopes are prominent tools in plant ecophysiology, biogeochemistry and in paleo research, but in all applications to date, isotope ratios of whole molecules are measured. However, it is well established that stable isotope abundance varies among intramolecular groups of biochemical metabolites, that is each so-called "isotopomer" has a distinct abundance. This intramolecular variation carries information on metabolic regulation, which can even be traced to individual enzymes (Schleucher et al., Plant, Cell Environ 1999). Here, we apply intramolecular isotope distributions to study the metabolic response of plants to increasing atmospheric [CO2] during the past century. Greenhouse experiments show that the deuterium abundance among the two positions in the C6H2 group of photosynthetic glucose depends on [CO2] during growth. This is observed for all plants using C3 photosynthesis, and reflects the metabolic flux ratio between photorespiration and photosynthesis. Photorespiration is a major C flux that limits assimilation in C3 plants, which encompass the overwhelming fraction of terrestrial photosynthesis and the

  5. THz time scale structural rearrangements and binding modes in lysozyme-ligand interactions. (United States)

    Woods, K N


    Predicting the conformational changes in proteins that are relevant for substrate binding is an ongoing challenge in the aim of elucidating the functional states of proteins. The motions that are induced by protein-ligand interactions are governed by the protein global modes. Our measurements indicate that the detected changes in the global backbone motion of the enzyme upon binding reflect a shift from the large-scale collective dominant mode in the unbound state towards a functional twisting deformation that assists in closing the binding cleft. Correlated motion in lysozyme has been implicated in enzyme function in previous studies, but detailed characterization of the internal fluctuations that enable the protein to explore the ensemble of conformations that ultimately foster large-scale conformational change is yet unknown. For this reason, we use THz spectroscopy to investigate the picosecond time scale binding modes and collective structural rearrangements that take place in hen egg white lysozyme (HEWL) when bound by the inhibitor (NAG)3. These protein thermal motions correspond to fluctuations that have a role in both selecting and sampling from the available protein intrinsic conformations that communicate function. Hence, investigation of these fast, collective modes may provide knowledge about the mechanism leading to the preferred binding process in HEWL-(NAG)3. Specifically, in this work we find that the picosecond time scale hydrogen-bonding rearrangements taking place in the protein hydration shell with binding modify the packing density within the hydrophobic core on a local level. These localized, intramolecular contact variations within the protein core appear to facilitate the large cooperative movements within the interfacial region separating the α- and β- domain that mediate binding. The THz time-scale fluctuations identified in the protein-ligand system may also reveal a molecular mechanism for substrate recognition.

  6. Time scales of foam stability in shallow conduits: Insights from analogue experiments (United States)

    Spina, L.; Scheu, B.; Cimarelli, C.; Arciniega-Ceballos, A.; Dingwell, D. B.


    Volcanic systems can exhibit periodical trends in degassing activity, characterized by a wide range of time scales. Understanding the dynamics that control such periodic behavior can provide a picture of the processes occurring in the feeding system. Toward this end, we analyzed the periodicity of outgassing in a series of decompression experiments performed on analogue material (argon-saturated silicone oil plus glass beads/fibers) scaled to serve as models of basaltic magma. To define the effects of liquid viscosity and crystal content on the time scale of outgassing, we investigated both: (1) pure liquid systems, at differing viscosities (100 and 1000 Pa s), and (2) particle-bearing suspensions (diluted and semidiluted). The results indicate that under dynamic conditions (e.g., decompressive bubble growth and fluid ascent within the conduit), the periodicity of foam disruption may be up to several orders of magnitude less than estimates based on the analysis of static conditions. This difference in foam disruption time scale is inferred to result from the contribution of bubble shear and bubble growth to inter-bubble film thinning. The presence of particles in the semidiluted regime is further linked to shorter bubble bursting times, likely resulting from contributions of the presence of a solid network and coalescence processes to the relative increase in bubble breakup rates. Finally, it is argued that these experiments represent a good analogue of gas-piston activity (i.e., the periodical rise-and-fall of a basaltic lava lake surface), implying a dominant role for shallow foam accumulation as a source process for these phenomena.

  7. Loschmidt echo in many-spin systems: contrasting time scales of local and global measurements. (United States)

    Zangara, Pablo R; Bendersky, Denise; Levstein, Patricia R; Pastawski, Horacio M


    A local excitation in a quantum many-spin system evolves deterministically. A time-reversal procedure, involving the inversion of the signs of every energy and interaction, should produce the excitation revival. This idea, experimentally coined in nuclear magnetic resonance, embodies the concept of the Loschmidt echo (LE). While such an implementation involves a single spin autocorrelation M(1,1), i.e. a local LE, theoretical efforts have focused on the study of the recovery probability of a complete many-body state, referred to here as global or many-body LE MMB Here, we analyse the relation between these magnitudes, with regard to their characteristic time scales and their dependence on the number of spins N We show that the global LE can be understood, to some extent, as the simultaneous occurrence of N independent local LEs, i.e. MMB∼(M(1,1))(N/4) This extensive hypothesis is exact for very short times and confirmed numerically beyond such a regime. Furthermore, we discuss a general picture of the decay of M1,1 as a consequence of the interplay between the time scale that characterizes the reversible interactions (T(2)) and that of the perturbation (τ(Σ)). Our analysis suggests that the short-time decay, characterized by the time scale τ(Σ), is greatly enhanced by the complex processes that occur beyond T(2) This would ultimately lead to the experimentally observed T(3), which was found to be roughly independent of τ(Σ) but closely tied to T(2).

  8. The Steppengrille (Gryllus spec./assimilis: selective filters and signal mismatch on two time scales.

    Directory of Open Access Journals (Sweden)

    Matti Michael Rothbart

    Full Text Available In Europe, several species of crickets are available commercially as pet food. Here we investigated the calling song and phonotactic selectivity for sound patterns on the short and long time scales for one such a cricket, Gryllus spec., available as "Gryllus assimilis", the Steppengrille, originally from Ecuador. The calling song consisted of short chirps (2-3 pulses, carrier frequency: 5.0 kHz emitted with a pulse period of 30.2 ms and chirp rate of 0.43 per second. Females exhibited high selectivity on both time scales. The preference for pulse period peaked at 33 ms which was higher then the pulse period produced by males. Two consecutive pulses per chirp at the correct pulse period were already sufficient for positive phonotaxis. The preference for the chirp pattern was limited by selectivity for small chirp duty cycles and for chirp periods between 200 ms and 500 ms. The long chirp period of the songs of males was unattractive to females. On both time scales a mismatch between the song signal of the males and the preference of females was observed. The variability of song parameters as quantified by the coefficient of variation was below 50% for all temporal measures. Hence, there was not a strong indication for directional selection on song parameters by females which could account for the observed mismatch. The divergence of the chirp period and female preference may originate from a founder effect, when the Steppengrille was cultured. Alternatively the mismatch was a result of selection pressures exerted by commercial breeders on low singing activity, to satisfy customers with softly singing crickets. In the latter case the prominent divergence between male song and female preference was the result of domestication and may serve as an example of rapid evolution of song traits in acoustic communication systems.

  9. Muscles innervated by a single motor neuron exhibit divergent synaptic properties on multiple time scales. (United States)

    Blitz, Dawn M; Pritchard, Amy E; Latimer, John K; Wakefield, Andrew T


    Adaptive changes in the output of neural circuits underlying rhythmic behaviors are relayed to muscles via motor neuron activity. Pre- and postsynaptic properties of neuromuscular junctions can impact the transformation from motor neuron activity to muscle response. Further, synaptic plasticity occurring on the time scale of inter-spike intervals can differ between multiple muscles innervated by the same motor neuron. In rhythmic behaviors, motor neuron bursts can elicit additional synaptic plasticity. However, it is unknown if plasticity regulated by the longer time scale of inter-burst intervals also differs between synapses from the same neuron, and whether any such distinctions occur across a physiological activity range. To address these issues, we measured electrical responses in muscles innervated by a chewing circuit neuron, the lateral gastric (LG) motor neuron, in a well-characterized small motor system, the stomatogastric nervous system (STNS) of the Jonah crab, Cancer borealis In vitro and in vivo, sensory, hormonal and modulatory inputs elicit LG bursting consisting of inter-spike intervals of 50-250 ms and inter-burst intervals of 2-24 s. Muscles expressed similar facilitation measured with paired stimuli except at the shortest inter-spike interval. However distinct decay time constants resulted in differences in temporal summation. In response to bursting activity, augmentation occurred to different extents and saturated at different inter-burst intervals in the three muscles. Further, augmentation interacted with facilitation, resulting in distinct intra-burst facilitation between muscles. Thus, responses of multiple target muscles diverge across a physiological activity range due to distinct synaptic properties sensitive to multiple time scales.

  10. On the time-scales of magmatism at island-arc volcanoes. (United States)

    Turner, S P


    Precise information on time-scales and rates of change is fundamental to an understanding of natural processes and the development of quantitative physical models in the Earth sciences. U-series isotope studies are revolutionizing this field by providing time information in the range 10(2)-10(4) years, which is similar to that of many modern Earth processes. I review how the application of U-series isotopes has been used to constrain the time-scales of magma formation, ascent and storage beneath island-arc volcanoes. Different elements are distilled-off the subducting plate at different times and in different places. Contributions from subducted sediments to island-arc lava sources appear to occur some 350 kyr to 4 Myr prior to eruption. Fluid release from the subducting oceanic crust into the mantle wedge may be a multi-stage process and occurs over a period ranging from a few hundred kyr to less than one kyr prior to eruption. This implies that dehydration commences prior to the initiation of partial melting within the mantle wedge, which is consistent with recent evidence that the onset of melting is controlled by an isotherm and thus the thermal structure within the wedge. U-Pa disequilibria appear to require a component of decompression melting, possibly due to the development of gravitational instabilities. The preservation of large (226)Ra disequilibria permits only a short period of time between fluid addition and eruption. This requires rapid melt segregation, magma ascent by channelled flow and minimal residence time within the lithosphere. The evolution from basalt to basaltic andesite probably occurs rapidly during ascent or in magma reservoirs inferred from some geophysical data to lie within the lithospheric mantle. The flux across the Moho is broadly andesitic, and some magmas subsequently stall in more shallow crustal-level magma chambers, where they evolve to more differentiated compositions on time-scales of a few thousand years or less.

  11. Green chromatography. (United States)

    Płotka, Justyna; Tobiszewski, Marek; Sulej, Anna Maria; Kupska, Magdalena; Górecki, Tadeusz; Namieśnik, Jacek


    Analysis of organic compounds in samples characterized by different composition of the matrix is very important in many areas. A vast majority of organic compound determinations are performed using gas or liquid chromatographic methods. It is thus very important that these methods have negligible environmental impact. Chromatographic techniques have the potential to be greener at all steps of the analysis, from sample collection and preparation to separation and final determination. The paper summarizes the approaches used to accomplish the goals of green chromatography. While complete elimination of sample preparation would be an ideal approach, it is not always practical. Solventless extraction techniques offer a very good alternative. Where solvents must be used, the focus should be on the minimization of their consumption. The approaches used to make chromatographic separations greener differ depending on the type of chromatography. In gas chromatography it is advisable to move away from using helium as the carrier gas because it is a non-renewable resource. GC separations using low thermal mass technology can be greener because of energy savings offered by this technology. In liquid chromatography the focus should be on the reduction of solvent consumption and replacement of toxic and environmentally hazardous solvents with more benign alternatives. Multidimensional separation techniques have the potential to make the analysis greener in both GC and LC. The environmental impact of the method is often determined by the location of the instrument with respect to the sample collection point.

  12. Ion Chromatography. (United States)

    Mulik, James D.; Sawicki, Eugene


    Accurate for the analysis of ions in solution, this form of analysis enables the analyst to directly assay many compounds that previously were difficult or impossible to analyze. The method is a combination of the methodologies of ion exchange, liquid chromatography, and conductimetric determination with eluant suppression. (Author/RE)

  13. Optically stimulated phosphorescence in orthoclase feldspar over the millisecond to second time scale

    DEFF Research Database (Denmark)

    Ankjærgaard, Christina; Jain, Mayank


    In the past, time-resolved IR stimulated luminescence (TR-IRSL) curves from feldspar have mainly been measured over a few hundred μs with the purpose of estimating the lifetimes of the components. In this study, we present the decay form of time-resolved IRSL and IR stimulated phosphorescence (IRSP......) from orthoclase feldspar covering over 8 orders of magnitude (50 ns to 7 s). A detailed characterisation of the slowly decaying signals (ms to s time scales) from feldspar is undertaken to obtain further insight into the role of re-trapping in both the IR stimulated luminescence (IRSL...

  14. Stability and periodicity of solutions for delay dynamic systems on time scales

    Directory of Open Access Journals (Sweden)

    Zhi-Qiang Zhu


    Full Text Available This article concerns the stability and periodicity of solutions to the delay dynamic system $$ x^{\\triangle}(t=A(t x(t + F(t, x(t, x(g(t+C(t $$ on a time scale. By the inequality technique for vectors, we obtain some stability criteria for the above system. Then, by using the Horn fixed point theorem, we present some conditions under which our system is asymptotically periodic and its periodic solution is unique. In particular, the periodic solution is positive under proper assumptions.

  15. Wildfire Disturbance and Sediment Transfers over Millennial Time Scales: A Numerical Modelling Study (United States)

    Martin, Y.


    Wildfire may lead to accelerated soil erosion, debris flow and shallow landsliding activity in the years following disturbance. This study focuses on coastal drainage basins in British Columbia over millennial time scales, for which accelerated rates of shallow landsliding following wildfire may be of particular significance. An algorithm for wildfire occurrence, based on lake and sediment charcoal studies undertaken in coastal British Columbia and western Washington over millennial time scales (for example, Gavin et al., 2003), is incorporated into a numerical model of sediment routing over these same time scales. A stochastic rule set for wildfire frequency, based on a Weibull distribution of fire return intervals, assigns years of fire occurrence in the model. In terms of location, south-facing aspects are assigned a 25 times greater susceptibility to wildfire than north-facing aspects. As a first-order approximation, it is supposed that loss of tree root strength resulting from stand-replacing wildfires is comparable in its effects to clearcut logging. Therefore, documentation of increased shallow landslide activity associated with logging is used to adjust landsliding transport equations for the years following wildfire disturbance. Thereafter, landsliding rates are returned to pre-disturbance values. Fire return intervals, particularly those on north-facing aspects, can be relatively long in coastal British Columbia when compared to return intervals typically found in drier mountain ranges. This study investigates the degree to which wildfire disturbance affects sediment routing and delivery to channels over millennial time scales in coastal British Columbia. Sensitivity to model parameters is evaluated. Further investigations of wildfire effects on geomorphic process operation will lead to improved understanding of natural disturbance regimes to which ecosystems adjust over both the short and long term. Such information can be used to evaluate possible

  16. Transient time-domain resonances and the time scale for tunneling

    CERN Document Server

    García-Calderón, G; Garc\\'{\\i}a-Calder\\'on, Gast\\'on; Villavicencio, Jorge


    Transient {\\it time-domain resonances} found recently in time-dependent solutions to Schr\\"{o}dinger's equation are used to investigate the issue of the tunneling time in rectangular potential barriers. In general, a time frequency analysis shows that these transients have frequencies above the cutoff frequency associated with the barrier height, and hence correspond to non-tunneling processes. We find, however, a regime characterized by the barrier opacity, where the peak maximum $t_{max}$ of the {\\it time-domain resonance} corresponds to under-the-barrier tunneling. We argue that $t_{max}$ represents the relevant tunneling time scale through the classically forbidden region.

  17. Resonance Raman kinetic spectroscopy of bacteriorhodopsin on the microsecond time scale

    Energy Technology Data Exchange (ETDEWEB)

    Campion, A.; El-Sayed, M.A.; Terner, J.


    Using a rotating disk with a slit of variable width, a continuous wave argon ion laser, and an Optical Multichanel Analyzer for detection, a new technique is reported which should, in principle, be capable of recording resonance Raman spectra with time resolution of 100 ns. The resonance Raman spectra of the intermediates of the photosynthetic cycle of bacteriorhodopsin are recorded on the microsecond time scale. Both the kinetic results and the resonance enhancement profile suggest that deprotonation results in an intermediate preceding bM/sub 412/ that has an optical absorption maximum at a wavelength longer than that of bM/sub 412/.

  18. Comparing trends in hydrometeorological average and extreme data sets around the world at different time scales

    Directory of Open Access Journals (Sweden)

    Thomas Rosmann


    New hydrological insights for the region: Results indicate that trends can be found for all variables and on all latitudes, with an increase of global temperature in the analysed time period. Fewer trends were observed in extreme value data. Trends in discharge data were predominantly negative, and precipitation trends were not very common. In some cases, an opposing pattern was observed in the northern and southern hemisphere. The highest number of trends was found at the annual and least on the daily resolution, nevertheless, trend patterns for discharges remained similar at different time scales. Some of the factors that might influence these results are discussed.

  19. Dimensional Reduction for Filters of Nonlinear Systems with Time-Scale Separation (United States)


    Rapp, Edwin Kreuzer and N. Sri Namachchivaya, “Reduced Nor- mal Forms for Nonlinear Control of Underactuated Hoisting Systems ,” Archive of Applied Mechanics , Vol.82, 2012, pp. 297 - 315. 7 ... Mechanics , Vol. 78(6), 2011, pp. 61001-1 - 61001-10. 8. Lee DeVille, N. Sri Namachchivaya and Zoi Rapti, “Noisy Two Dimensional Non-Hamiltonian System ...AFRL-OSR-VA-TR-2013-0009 Dimensional Reduction for Filters of Nonlinear Systems with Time- Scale Separation Namachchivaya, N

  20. Oscillation of Third-order Delay Dynamic Equations on Time Scales

    Institute of Scientific and Technical Information of China (English)

    YANG Jia-shan


    This paper is concerned with the oscillatory behavior of a class of third-order nonlinear variable delay neutral functional dynamic equations on time scale. By using the generalized Riccati transformation and inequality technique, we establish some new oscilla-tion criteria for the equations. Our results extend and improve some known results, but also unify the oscillation of third-order nonlinear variable delay functional differential equations and functional difference equations with a nonlinear neutral term. Some examples are given to illustrate the importance of our results.

  1. Hamilton-Jacobi equation, heteroclinic chains and Arnol'd diffusion in three time scales systems

    CERN Document Server

    Gallavotti, G; Mastropietro, V; Gallavotti, Giovanni; Gentile, Guido; Mastropietro, Vieri


    Interacting systems consisting of two rotators and a point mass near a hyperbolic fixed point are considered, in a case in which the uncoupled systems have three very different characteristic time scales. The abundance of quasi periodic motions in phase space is studied via the Hamilton-Jacobi equation. The main result, a high density theorem of invariant tori, is derived by the classical canonical transformation method extending previous results. As an application the existence of long heteroclinic chains (and of Arnol'd diffusion) is proved for systems interacting through a trigonometric polynomial in the angle variables.

  2. Time Scale Analysis of Interest Rate Spreads and Output Using Wavelets

    Directory of Open Access Journals (Sweden)

    Marco Gallegati


    Full Text Available This paper adds to the literature on the information content of different spreads for real activity by explicitly taking into account the time scale relationship between a variety of monetary and financial indicators (real interest rate, term and credit spreads and output growth. By means of wavelet-based exploratory data analysis we obtain richer results relative to the aggregate analysis by identifying the dominant scales of variation in the data and the scales and location at which structural breaks have occurred. Moreover, using the “double residuals” regression analysis on a scale-by-scale basis, we find that changes in the spread in several markets have different information content for output at different time frames. This is consistent with the idea that allowing for different time scales of variation in the data can provide a fruitful understanding of the complex dynamics of economic relationships between variables with non-stationary or transient components, certainly richer than those obtained using standard time domain methods.

  3. Characterization of Microbial Fuel Cells at Microbially and Electrochemically Meaningful Time scales

    KAUST Repository

    Ren, Zhiyong


    The variable biocatalyst density in a microbial fuel cell (MFC) anode biofilm is a unique feature of MFCs relative to other electrochemical systems, yet performance characterizations of MFCs typically involve analyses at electrochemically relevant time scales that are insufficient to account for these variable biocatalyst effects. This study investigated the electrochemical performance and the development of anode biofilm architecture under different external loadings, with duplicate acetate-fed singlechamber MFCs stabilized at each resistance for microbially relevant time scales. Power density curves from these steady-state reactors generally showed comparable profiles despite the fact that anode biofilm architectures and communities varied considerably, showing that steady-state biofilm differences had little influence on electrochemical performance until the steady-state external loading was much larger than the reactor internal resistance. Filamentous bacteria were dominant on the anodes under high external resistances (1000 and 5000 Ω), while more diverse rod-shaped cells formed dense biofilms under lower resistances (10, 50, and 265 Ω). Anode charge transfer resistance decreased with decreasing fixed external resistances, but was consistently 2 orders of magnitude higher than the resistance at the cathode. Cell counting showed an inverse exponential correlation between cell numbers and external resistances. This direct link ofMFCanode biofilm evolution with external resistance and electricity production offers several operational strategies for system optimization. © 2011 American Chemical Society.

  4. Stability of conditionally invariant sets and controlled uncertain dynamic systems on time scales

    Directory of Open Access Journals (Sweden)

    Lakshmikantham V.


    Full Text Available A basic feedback control problem is that of obtaining some desired stability property from a system which contains uncertainties due to unknown inputs into the system. Despite such imperfect knowledge in the selected mathematical model, we often seek to devise controllers that will steer the system in a certain required fashion. Various classes of controllers whose design is based on the method of Lyapunov are known for both discrete [4], [10], [15], and continuous [3–9], [11] models described by difference and differential equations, respectively. Recently, a theory for what is known as dynamic systems on time scales has been built which incorporates both continuous and discrete times, namely, time as an arbitrary closed sets of reals, and allows us to handle both systems simultaneously [1], [2], [12], [13]. This theory permits one to get some insight into and better understanding of the subtle differences between discrete and continuous systems. We shall, in this paper, utilize the framework of the theory of dynamic systems on time scales to investigate the stability properties of conditionally invariant sets which are then applied to discuss controlled systems with uncertain elements. For the notion of conditionally invariant set and its stability properties, see [14]. Our results offer a new approach to the problem in question.

  5. Coarse-Grained Modeling of Genetic Circuits as a Function of the Inherent Time Scales

    CERN Document Server

    Labavic, Darka; Hildegard, Wolfhard Janke; Meyer-Ortmanns,


    From a coarse-grained perspective the motif of a self-activating species, activating a second species which acts as its own repressor, is widely found in biological systems, in particular in genetic systems with inherent oscillatory behavior. Here we consider a specific realization of this motif as a genetic circuit, in which genes are described as directly producing proteins, leaving out the intermediate step of mRNA production. We focus on the effect that inherent time scales on the underlying fine-grained scale can have on the bifurcation patterns on a coarser scale in time. Time scales are set by the binding and unbinding rates of the transcription factors to the promoter regions of the genes. Depending on the ratio of these rates to the decay times of the proteins, the appropriate averaging procedure for obtaining a coarse-grained description changes and leads to sets of deterministic equations, which differ in their bifurcation structure. In particular the desired intermediate range of regular limit cyc...

  6. Interdecadal Variations of Phase Delays Between Two Ni(n)o Indices at Different Time Scales

    Institute of Scientific and Technical Information of China (English)

    BIAN Jianchun; YANG Peicai


    Phase delays between two Nino indices-sea surface temperatures in Nino regions 1+2 and 3.4(1950-2001)-at different time scales are detected by wavelet analysis. Analysis results show that thereare two types of period bifurcations in the Nino indices and that period bifurcation points exist only in the region where the wavelet power is small. Interdecadal variation features of phase delays between the two indices vary with different time scales. In the periods of 40-72 months, the phase delay changes its sign in 1977: Nino 1+2 indices are 2-4 months earlier than Nino 3.4 indices before 1977, but 3-6 months later afterwards. In the periods of 20-40 months, however, the phase delay changes its sign in another way:Nino 1+2 indices are 1-4 months earlier before 1980 and during 1986-90, but 1-4 months later during 1980-83 and 1993-2001.

  7. Time scaling with efficient time-propagation techniques for atoms and molecules in pulsed radiation fields

    CERN Document Server

    Hamido, Aliou; Madroñero, Javier; Mota-Furtado, Francisca; O'Mahony, Patrick; Frapiccini, Ana Laura; Piraux, Bernard


    We present an ab initio approach to solve the time-dependent Schr\\"odinger equation to treat electron and photon impact multiple ionization of atoms or molecules. It combines the already known time scaled coordinate method with a new high order time propagator based on a predictor-corrector scheme. In order to exploit in an optimal way the main advantage of the time scaled coordinate method namely that the scaled wave packet stays confined and evolves smoothly towards a stationary state the modulus square of which being directly proportional to the electron energy spectra in each ionization channel, we show that the scaled bound states should be subtracted from the total scaled wave packet. In addition, our detailed investigations suggest that multi-resolution techniques like for instance, wavelets are the most appropriate ones to represent spatially the scaled wave packet. The approach is illustrated in the case of the interaction of an one-dimensional model atom as well as atomic hydrogen with a strong osci...

  8. Long time-scale variability of X-ray binaries with late type giant companions

    CERN Document Server

    Filippova, E; Parkin, E R


    In this paper we propose and examine a physical mechanism which can lead to the generation of noise in the mass accretion rate of low mass X-ray binaries on time-scales comparable to the orbital period of the system. We consider modulations of mass captured by the compact object from the companion star's stellar wind in binaries with late type giants, systems which usually have long orbital periods. We show that a hydrodynamical interaction of the wind matter within a binary system even without eccentricity results in variability of the mass accretion rate with characteristic time-scales close to the orbital period. The cause of the variability is an undeveloped turbulent motion (perturbed motion without significant vorticity) of wind matter near the compact object. Our conclusions are supported by 3D simulations with two different hydrodynamic codes based on Lagrangian and Eulerian approaches -- the SPH code GADGET and the Eulerian code PLUTO. In this work we assume that the wind mass loss rate of the second...

  9. Atomistic protein folding simulations on the submillisecond time scale using worldwide distributed computing. (United States)

    Pande, Vijay S; Baker, Ian; Chapman, Jarrod; Elmer, Sidney P; Khaliq, Siraj; Larson, Stefan M; Rhee, Young Min; Shirts, Michael R; Snow, Christopher D; Sorin, Eric J; Zagrovic, Bojan


    Atomistic simulations of protein folding have the potential to be a great complement to experimental studies, but have been severely limited by the time scales accessible with current computer hardware and algorithms. By employing a worldwide distributed computing network of tens of thousands of PCs and algorithms designed to efficiently utilize this new many-processor, highly heterogeneous, loosely coupled distributed computing paradigm, we have been able to simulate hundreds of microseconds of atomistic molecular dynamics. This has allowed us to directly simulate the folding mechanism and to accurately predict the folding rate of several fast-folding proteins and polymers, including a nonbiological helix, polypeptide alpha-helices, a beta-hairpin, and a three-helix bundle protein from the villin headpiece. Our results demonstrate that one can reach the time scales needed to simulate fast folding using distributed computing, and that potential sets used to describe interatomic interactions are sufficiently accurate to reach the folded state with experimentally validated rates, at least for small proteins.

  10. The learning of isometric force time scales is differentially influenced by constant and variable practice. (United States)

    King, Adam C; Newell, Karl M


    This experiment was set up to investigate the influence of constant and variable practice on performance accuracy and the time- and frequency-dependent structure of the force output dynamics in the learning of an irregular isometric force pattern. Traditional approaches to the variability of practice hypothesis have demonstrated benefits of task-induced variability at the outcome level of behavior, but there have been limited investigations of the effect of practice conditions on movement execution and particularly the multiple time scale processes of force output. During the practice phase, variability was induced along the force-time dimension of the target pattern for the variable practice condition (different wave forms), but the wave forms exhibited the same distributional properties of the frequency content (1/f noise: β = -1.5) as the constant practice condition. The results showed that both practice conditions exhibited similar reductions in task error as a function of practice. However, constant practice produced greater changes in the time- and frequency-dependent properties of force output than variable practice, including a higher relative change in the contribution from faster (4-12 Hz) time scale mechanisms. Generalization tests to novel target patterns revealed that the task dynamics had a greater influence than the effect of practice conditions. Collectively, the findings support the adaptive nature of force output structure and the perspective that practice conditions can produce differential effects on the outcome and execution levels of motor behavior.

  11. Weyl-Titchmarsh Theory for Time Scale Symplectic Systems on Half Line

    Directory of Open Access Journals (Sweden)

    Roman Šimon Hilscher


    Full Text Available We develop the Weyl-Titchmarsh theory for time scale symplectic systems. We introduce the M(λ-function, study its properties, construct the corresponding Weyl disk and Weyl circle, and establish their geometric structure including the formulas for their center and matrix radii. Similar properties are then derived for the limiting Weyl disk. We discuss the notions of the system being in the limit point or limit circle case and prove several characterizations of the system in the limit point case and one condition for the limit circle case. We also define the Green function for the associated nonhomogeneous system and use its properties for deriving further results for the original system in the limit point or limit circle case. Our work directly generalizes the corresponding discrete time theory obtained recently by S. Clark and P. Zemánek (2010. It also unifies the results in many other papers on the Weyl-Titchmarsh theory for linear Hamiltonian differential, difference, and dynamic systems when the spectral parameter appears in the second equation. Some of our results are new even in the case of the second-order Sturm-Liouville equations on time scales.

  12. Multiple Time Scale Analysis of River Runoff Using Wavelet Transform for Dagujia River Basin, Yantai, China

    Institute of Scientific and Technical Information of China (English)

    LIU Delin; LIU Xianzhao; LI Bicheng; ZHAO Shiwei; LI Xiguo


    Based on monOdy river runoff and meteorological data, a method of Morlet wavelet transform was used to analyze the multiple time scale characteristics of river runoffin the Dagnjia River Basin, Yantai City, Shandong Province. The results showed that the total annual river runoff in the Dagujia River Basin decreased significantly from 1966 to 2004, and the rate of decrease was 48×106m3/10yr, which was higher than the mean value of most rivers in China. Multiple time scale characteristics existed, which accounted for different aspects of the changes in annual river runoff, and the major periods of the runoff time series were identified as about 28 years, 14 years and 4 years with decreasing levels of fluctuation. The river runoff evolution process was controlled by changes in precipitation to a certain extent, but it was also greatly influenced by human activities. Also, for different time periods and scales, the impacts of climate changes and human activities on annual river runoff evolution occurred at the same time. Changes in the annual river runoffwere mainly associated with climate change before the 1980s and with human activities after 1981.

  13. Time-Scale Domain Characterization of Time-Varying Ultrawideband Infostation Channel

    Directory of Open Access Journals (Sweden)

    U.A.K. Chude-Okonkwo


    Full Text Available The time-scale domain geometrical-based method for the characterization of the time varying ultrawideband (UWB channel typical of an infostation channel is presented. Compared to methods that use Doppler shift as a measure of time-variation in the channel this model provides a more reliable measure of frequency dispersion caused by terminal mobility in the UWB infostation channel. Particularly, it offers carrier frequency independent method of computing wideband channel responses and parameters which are important for ultrawideband systems. Results show that the frequency dispersion of the channel depends on the frequency and not on the choice of bandwidth. And time dispersion depends on bandwidth and not on the frequency. It is also shown that for time-varying UWB, frame length defined over the coherence time obtained with reference to the carrier frequency results in an error margin which can be reduced by using the coherence time defined with respect to the maximum frequency in a given frequency band. And the estimation of the frequency offset using the time-scale domain (wideband model presented here (especially in the case of multiband UWB frequency synchronization is more accurate than using frequency offset estimate obtained from narrowband models.

  14. Formation of the Kuiper Belt by Long Time-Scale Migration of Jovian Planets

    Institute of Scientific and Technical Information of China (English)

    Jian Li; Li-Yong Zhou; Yi-Sui Sun


    The orbital migration of Jovian planets is believed to have played an important role in shaping the Kuiper Belt. We investigate the effects of the long time-scale (2 × 107 yr) migration of Jovian planets on the orbital evolution of massless test particles that are initially located beyond 28 AU. Because of the slowness of the migration, Neptune's mean motion resonances capture test particles very efficiently. Taking into account the stochastic behavior during the planetary migration and for proper parameter values, the resulting concentration of objects in the 3:2 resonance is prominent, while very few objects enter the 2:1 resonance,thus matching the observed Kuiper Belt objects very well. We also find that such a long time-scale migration is favorable for exciting the inclinations of the test particles, because it makes the secular resonance possible to operate during the migration. Our analyses show that the v8 secular resonance excites the eccentricities of some test particles, so decreasing their perihelion distances, leading to close encounters with Neptune, which can then pump the inclinations up to 20°.

  15. Relativistic electron acceleration and decay time scales in the inner and outer radiation belts: SAMPEX (United States)

    Baker, D. N.; Blake, J. B.; Callis, L. B.; Cummings, J. R.; Hovestadt, D.; Kanekal, S.; Klecker, B.; Mewaldt, R. A.; Zwickl, R. D.


    High-energy electrons have been measured systematically in a low-altitude (520 x 675 km), nearly polar (inclination = 82 deg) orbit by sensitive instruments onboard the Solar, Anomalous, and Magnetospheric Particle Explorer (SAMPEX). Count rate channels with electron energy thresholds ranging from 0.4 MeV to 3.5 MeV in three different instruments have been used to examine relativistic electron variations as a function of L-shell parameter and time. A long run of essentially continuous data (July 1992 - July 1993) shows substantial acceleration of energetic electrons throughout much of the magnetosphere on rapid time scales. This acceleration appears to be due to solar wind velocity enhancements and is surprisingly large in that the radiation belt 'slot' region often is filled temporarily and electron fluxes are strongly enhanced even at very low L-values (L aprroximately 2). A superposed epoch analysis shows that electron fluxes rise rapidly for 2.5 is approximately less than L is approximately less than 5. These increases occur on a time scale of order 1-2 days and are most abrupt for L-values near 3. The temporal decay rate of the fluxes is dependent on energy and L-value and may be described by J = Ke-t/to with t(sub o) approximately equals 5-10 days. Thus, these results suggest that the Earth's magnetosphere is a cosmic electron accelerator of substantial strength and efficiency.

  16. Protein purification using PDZ affinity chromatography. (United States)

    Walkup, Ward G; Kennedy, Mary B


    PDZ domains function in nature as protein-binding domains within scaffold and membrane-associated proteins. They comprise approximately 90 residues and undergo specific, high-affinity interactions with complementary C-terminal peptide sequences, other PDZ domains, and/or phospholipids. We have previously shown that the specific, strong interactions of PDZ domains with their ligands make them well suited for use in affinity chromatography. This unit provides protocols for the PDZ affinity chromatography procedure that are applicable for the purification of proteins that contain PDZ domains or PDZ domain-binding ligands, either naturally or introduced by genetic engineering. We detail the preparation of affinity resins composed of PDZ domains or PDZ domain peptide ligands coupled to solid supports. These resins can be used to purify proteins containing endogenous or genetically introduced PDZ domains or ligands, eluting the proteins with free PDZ domain peptide ligands.

  17. 高效液相色谱法测定多肽AP25的含量及有关物质%Content Determination of Peptide AP25 And Its Related Substances by High Performance Liquid Chromatography

    Institute of Scientific and Technical Information of China (English)

    尼格尔热依·亚迪卡尔; 徐寒梅


    建立多肽AP25含量测定及有关物质检查的高效液相色谱方法.采用COSMOSIL C18色谱柱(250 mm×4.6mm,5μm);以0.05 mol/L磷酸二氢钠(用磷酸调pH值至2.5)为流动相A,乙腈为流动相B,进行梯度洗脱;检测波长为220 nm;体积流量为1.0 mL/min;柱温为25℃;进样体积为20μL.在该色谱条件下,各有关物质峰均可与AP25主峰良好分离,理论塔板数不低于5 000,AP25浓度在0.8 ~1.2 mg/mL(r =0.999 6,n=5)和18~42μg/mL(r =0.999 5,n=5)范围内与峰面积呈良好线性关系;检测限和定量限分别为0.5ng和1ng;含量和有关物质检测方法的重复性试验RSD值分别为0.60%和0.48%(n=6),中间精密度试验RSD值分别为0.35%和0.80%(n=12).3批原料药的含量和有关物质检测结果分别为99.56%,99.84%,99.74%和2.17%,0.98%,2.19%.本方法简便易行,灵敏度高,耐用性、专属性良好,准确稳定,可用于测定多肽AP25的含量及有关物质.%To establish a high performance liquid chromatography (HPLC) method for content determination of the peptide AP25 and its related substances,the separation was performed on a COSMOSIL C18 column (250 mm × 4.6mm,5 μm).The mobile phase A consisted of 0.05 mol/L sodium dihydrogen phosphate (adjusting the pH value to 2.5 with the phosphonic acid),the mobile phase B was acetonitrile,the separation process was gradient elution:the ratio of mobile phase B rised from 10% to 40% within 30 minutes.The retention time of AP25 main peak was kept at about 13 minutes.The detection wavelength was at 220 nm and the flow rate was 1.0 mL/min,the column temperature was 25 ℃,and the volume of injection was 20 μL.Under this chromatographic condition,an excellent separation was achieved for AP25 and its related substances,the degree of separability was more than 1.5,and the number of theoretical plates with AP25 was more than 5 000.There was a good linear relationship between the peak area and the concentration of AP25

  18. Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography* (United States)

    Huang, Honggang; Haar Petersen, Martin; Ibañez-Vea, Maria; Lassen, Pernille S.; Larsen, Martin R.; Palmisano, Giuseppe


    Cysteine is a rare and conserved amino acid involved in most cellular functions. The thiol group of cysteine can be subjected to diverse oxidative modifications that regulate many physio-pathological states. In the present work, a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) was synthesized to selectively label cysteine-containing peptides (Cys peptides) followed by their enrichment with titanium dioxide (TiO2) and subsequent mass spectrometric analysis. The CysPAT strategy was developed using a synthetic peptide, a standard protein and subsequently the strategy was applied to protein lysates from Hela cells, achieving high specificity and enrichment efficiency. In particular, for Cys proteome analysis, the method led to the identification of 7509 unique Cys peptides from 500 μg of HeLa cell lysate starting material. Furthermore, the method was developed to simultaneously enrich Cys peptides and phosphorylated peptides. This strategy was applied to SILAC labeled Hela cells subjected to 5 min epidermal growth factor (EGF) stimulation. In total, 10440 unique reversibly modified Cys peptides (3855 proteins) and 7339 unique phosphopeptides (2234 proteins) were simultaneously identified from 250 μg starting material. Significant regulation was observed in both phosphorylation and reversible Cys modification of proteins involved in EGFR signaling. Our data indicates that EGF stimulation can activate the well-known phosphorylation of EGFR and downstream signaling molecules, such as mitogen-activated protein kinases (MAPK1 and MAPK3), however, it also leads to substantial modulation of reversible cysteine modifications in numerous proteins. Several protein tyrosine phosphatases (PTPs) showed a reduction of the catalytic Cys site in the conserved putative phosphatase HC(X)5R motif indicating an activation and subsequent de-phosphorylation of proteins involved in the EGF signaling pathway. Overall, the CysPAT strategy is a straight forward, easy and promising

  19. Atomic Force Microscope Mediated Chromatography (United States)

    Anderson, Mark S.


    The atomic force microscope (AFM) is used to inject a sample, provide shear-driven liquid flow over a functionalized substrate, and detect separated components. This is demonstrated using lipophilic dyes and normal phase chromatography. A significant reduction in both size and separation time scales is achieved with a 25-micron-length column scale, and one-second separation times. The approach has general applications to trace chemical and microfluidic analysis. The AFM is now a common tool for ultra-microscopy and nanotechnology. It has also been demonstrated to provide a number of microfluidic functions necessary for miniaturized chromatography. These include injection of sub-femtoliter samples, fluidic switching, and sheardriven pumping. The AFM probe tip can be used to selectively remove surface layers for subsequent microchemical analysis using infrared and tip-enhanced Raman spectroscopy. With its ability to image individual atoms, the AFM is a remarkably sensitive detector that can be used to detect separated components. These diverse functional components of microfluidic manipulation have been combined in this work to demonstrate AFM mediated chromatography. AFM mediated chromatography uses channel-less, shear-driven pumping. This is demonstrated with a thin, aluminum oxide substrate and a non-polar solvent system to separate a mixture of lipophilic dyes. In conventional chromatographic terms, this is analogous to thin-layer chromatography using normal phase alumina substrate with sheardriven pumping provided by the AFM tip-cantilever mechanism. The AFM detection of separated components is accomplished by exploiting the variation in the localized friction of the separated components. The AFM tip-cantilever provides the mechanism for producing shear-induced flows and rapid pumping. Shear-driven chromatography (SDC) is a relatively new concept that overcomes the speed and miniaturization limitations of conventional liquid chromatography. SDC is based on a

  20. Simultaneous quantification of α-lactalbumin and β-casein in human milk using ultra-performance liquid chromatography with tandem mass spectrometry based on their signature peptides and winged isotope internal standards. (United States)

    Chen, Qi; Zhang, Jingshun; Ke, Xing; Lai, Shiyun; Li, Duo; Yang, Jinchuan; Mo, Weimin; Ren, Yiping


    In recent years, there is an increasing need to measure the concentration of individual proteins in human milk, instead of total human milk proteins. Due to lack of human milk protein standards, there are only few quantification methods established. The objective of the present work was to develop a simple and rapid quantification method for simultaneous determination of α-lactalbumin and β-casein in human milk using signature peptides according to a modified quantitative proteomics strategy. The internal standards containing the signature peptide sequences were synthesized with isotope-labeled amino acids. The purity of synthesized peptides as standards was determined by amino acid analysis method and area normalization method. The contents of α-lactalbumin and β-casein in human milk were measured according to the equimolar relationship between the two proteins and their corresponding signature peptides. The method validation results showed a satisfied linearity (R(2)>0.99) and recoveries (97.2-102.5% for α-lactalbumin and 99.5-100.3% for β-casein). The limit of quantification for α-lactalbumin and β-casein was 8.0mg/100g and 1.2mg/100g, respectively. CVs for α-lactalbumin and β-casein in human milk were 5.2% and 3.0%. The contents of α-lactalbumin and β-casein in 147 human milk samples were successfully determined by the established method and their contents were 205.5-578.2mg/100g and 116.4-467.4mg/100g at different lactation stages. The developed method allows simultaneously determination of α-lactalbumin and β-casein in human milk. The quantitative strategy based on signature peptide should be applicable to other endogenous proteins in breast milk and other body fluids.

  1. Identification of multiple antimicrobial peptides from the skin of fine-spined frog, Hylarana spinulosa (Ranidae). (United States)

    Yang, Xiaolong; Hu, Yuhong; Xu, Shiqi; Hu, Yonghong; Meng, Hao; Guo, Chao; Liu, Yuliang; Liu, Jingze; Yu, Zhijun; Wang, Hui


    In this study, peptidomics and genomics analyses were used to study antimicrobial peptides from the skin of Hylarana spinulosa. Twenty-nine different antimicrobial peptide precursors were characterized from the skin of H. spinulosa, which produce 23 mature antimicrobial peptides belonging to 12 different families. To confirm the actual presence and characteristics of these antimicrobial peptides in the skin tissue extractions from H. spinulosa, we used two distinct methods, one was peptide purification method that combined gel filtration chromatography and reversed-phase high performance liquid chromatography (RP-HPLC), and the other was peptidomics approach based on liquid chromatography in conjunction with tandem mass spectrometry (LC-MS/MS). In the peptidomics approach, incomplete tryptic digestion and gas-phase fractionation (GPF) analysis were used to increase peptidome coverage and reproducibility of peptide ion selection. Multiple species of microorganisms were chosen to test and analyze the antimicrobial activities and spectrum of these antimicrobial peptides.

  2. Efficient purification of unique antibodies using peptide affinity-matrix columns

    DEFF Research Database (Denmark)

    Jensen, Liselotte Brix; Riise, Erik; Nielsen, Leif Kofoed;


    -99. Several peptide epitopes were identified and all of them recognised specifically MK16. One peptide, ER6.1, was selected and linked to beaded agarose and demonstrated excellent performance as a peptide affinity chromatography matrix. This epitope matrix was efficient in the purification of MK16 Fab...

  3. Pleurostrin, an antifungal peptide from the oyster mushroom. (United States)

    Chu, K T; Xia, Lixin; Ng, T B


    A 7kDa peptide, with inhibitory activity on mycelial growth in the fungi Fusaerium oxysporum, Mycosphaerella arachidicola and Physalospora piricola, was isolated from fresh fruiting bodies of the oyster mushroom. The isolation procedure entailed extraction with an aqueous buffer, ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel and gel filtration by fast protein liquid chromatography on Superdex 75. The protein was unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel. It demonstrated an N-terminal sequence different from known antifungal proteins and peptides.

  4. Probing Time-Dependent Molecular Dipoles on the Attosecond Time Scale (United States)

    Neidel, Ch.; Klei, J.; Yang, C.-H.; Rouzée, A.; Vrakking, M. J. J.; Klünder, K.; Miranda, M.; Arnold, C. L.; Fordell, T.; L'Huillier, A.; Gisselbrecht, M.; Johnsson, P.; Dinh, M. P.; Suraud, E.; Reinhard, P.-G.; Despré, V.; Marques, M. A. L.; Lépine, F.


    Photoinduced molecular processes start with the interaction of the instantaneous electric field of the incident light with the electronic degrees of freedom. This early attosecond electronic motion impacts the fate of the photoinduced reactions. We report the first observation of attosecond time scale electron dynamics in a series of small- and medium-sized neutral molecules (N2, CO2, and C2H4), monitoring time-dependent variations of the parent molecular ion yield in the ionization by an attosecond pulse, and thereby probing the time-dependent dipole induced by a moderately strong near-infrared laser field. This approach can be generalized to other molecular species and may be regarded as a first example of molecular attosecond Stark spectroscopy.

  5. Asymptotic Expansions of Backward Equations for Two-time-scale Markov Chains in Continuous Time

    Institute of Scientific and Technical Information of China (English)

    G Yin; Dung Tien Nguyen


    This work develops asymptotic expansions for solutions of systems of backward equations of timeinhomogeneons Markov chains in continuous time. Owing to the rapid progress in technology and the increasing complexity in modeling, the underlying Markov chains often have large state spaces, which make the computational tasks infeasible. To reduce the complexity, two-time-scale formulations are used. By introducing a small parameter ε> 0 and using suitable decomposition and aggregation procedures, it is formulated as a singular perturbation problem. Both Markov chains having recurrent states only and Markov chains including also transient states are treated. Under certain weak irreducibility and smoothness conditions of the generators, the desired asymptotic expansions are constructed. Then error bounds are obtained.

  6. Fluctuations and time scales for bed-load sediment motion over a smooth bed

    Institute of Scientific and Technical Information of China (English)

    Francesco Ballio n; Alessio Radice


    Results are presented for experiments of bed-load sediment transport over a plane, smooth bed. The smooth-bed configuration, though not adequate for mimicking natural streams, enables the effects of bed roughness to be filtered out, thus, highlighting the role of flow turbulence for particle dynamics. Sediments were individually tracked along their paths, measuring position and velocity of the individual grains. A number of analyses were then applied to the data: probability density function, auto-correla-tion, and spectra of the grain velocity. Several Lagrangian time scales of particle motion were obtained and compared to available data for the turbulent flow field to determine a phenomenological inter-pretation of the process.

  7. Time-Scale and Time-Frequency Analyses of Irregularly Sampled Astronomical Time Series

    Directory of Open Access Journals (Sweden)

    S. Roques


    Full Text Available We evaluate the quality of spectral restoration in the case of irregular sampled signals in astronomy. We study in details a time-scale method leading to a global wavelet spectrum comparable to the Fourier period, and a time-frequency matching pursuit allowing us to identify the frequencies and to control the error propagation. In both cases, the signals are first resampled with a linear interpolation. Both results are compared with those obtained using Lomb's periodogram and using the weighted waveletZ-transform developed in astronomy for unevenly sampled variable stars observations. These approaches are applied to simulations and to light variations of four variable stars. This leads to the conclusion that the matching pursuit is more efficient for recovering the spectral contents of a pulsating star, even with a preliminary resampling. In particular, the results are almost independent of the quality of the initial irregular sampling.

  8. Effects of demographic stochasticity on biological community assembly on evolutionary time scales

    KAUST Repository

    Murase, Yohsuke


    We study the effects of demographic stochasticity on the long-term dynamics of biological coevolution models of community assembly. The noise is induced in order to check the validity of deterministic population dynamics. While mutualistic communities show little dependence on the stochastic population fluctuations, predator-prey models show strong dependence on the stochasticity, indicating the relevance of the finiteness of the populations. For a predator-prey model, the noise causes drastic decreases in diversity and total population size. The communities that emerge under influence of the noise consist of species strongly coupled with each other and have stronger linear stability around the fixed-point populations than the corresponding noiseless model. The dynamics on evolutionary time scales for the predator-prey model are also altered by the noise. Approximate 1/f fluctuations are observed with noise, while 1/ f2 fluctuations are found for the model without demographic noise. © 2010 The American Physical Society.

  9. The mechanism of bursting phenomena in Belousov-Zhabotinsky(BZ) chemical reaction with multiple time scales

    Institute of Scientific and Technical Information of China (English)


    The dynamics of a typical Belousov-Zhabotinsky(BZ)reaction with multiple time scales is investigated in this paper.Different forms of periodic bursting phenomena,and specially,three types of chaotic bursters with different structures can be obtained,which are in common with the behaviors observed in experiments.The bifurcations connecting the quiescent state and the repetitive spikes are presented to account for the occurrence of the NKoscillations as well as the different forms of chaotic bursters.The mechanism of the period-adding bifurcation sequences is explored to reveal why the length of the periods in the sequences does not change continuously with the continuous variation of the parameters.

  10. Breakup time scale studied in the 8 GeV/c $\\pi^{-}$ + $^{197}Au$ reaction

    CERN Document Server

    Pienkowski, L; Lefort, T; Hsi, W C; Beaulieu, L; Botvina, A; Back, B B; Breuer, H; Gushue, S; Korteling, R G; Laforest, R; Martin, E; Ramakrishnan, E; Remsberg, L P; Rowland, D; Ruangma, A; Viola, V E; Winchester, E M; Yennello, S J


    Experimental data from the reaction of an 8.0 GeV/c pi- beam incident on a 197Au target have been analyzed in order to investigate the integrated breakup time scale for hot residues. Alpha-particle energy spectra and particle angular distributions supported by a momentum tensor analysis suggest that at large excitation energy, above 3-5 MeV/nucleon, light-charged particles are emitted prior to or at the same time as the emission of the heavy fragments. Comparison with the SMM and GEMINI models is presented. A binary fission-like mechanism fits the experimental data at low excitation energies, but seems unable to reproduce the data at excitation energies above 3-5 MeV/nucleon.

  11. Time-resolved and time-scale adaptive measures of spike train synchrony

    CERN Document Server

    Kreuz, Thomas; Greschner, Martin; Andrzejak, Ralph G


    A wide variety of approaches to estimate the degree of synchrony between two or more spike trains have been proposed. One of the most recent methods is the ISI-distance which extracts information from the interspike intervals (ISIs) by evaluating the ratio of the instantaneous firing rates. In contrast to most previously proposed measures it is parameter free and time-scale independent. However, it is not well suited to track changes in synchrony that are based on spike coincidences. Here we propose the SPIKE-distance, a complementary measure which is sensitive to spike coincidences but still shares the fundamental advantages of the ISI-distance. In particular, it is easy to visualize in a time-resolved manner and can be extended to a method that is also applicable to larger sets of spike trains. We show the merit of the SPIKE-distance using both simulated and real data.

  12. Time-resolved and time-scale adaptive measures of spike train synchrony. (United States)

    Kreuz, Thomas; Chicharro, Daniel; Greschner, Martin; Andrzejak, Ralph G


    A wide variety of approaches to estimate the degree of synchrony between two or more spike trains have been proposed. One of the most recent methods is the ISI-distance which extracts information from the interspike intervals (ISIs) by evaluating the ratio of the instantaneous firing rates. In contrast to most previously proposed measures it is parameter free and time-scale independent. However, it is not well suited to track changes in synchrony that are based on spike coincidences. Here we propose the SPIKE-distance, a complementary measure which is sensitive to spike coincidences but still shares the fundamental advantages of the ISI-distance. In particular, it is easy to visualize in a time-resolved manner and can be extended to a method that is also applicable to larger sets of spike trains. We show the merit of the SPIKE-distance using both simulated and real data.

  13. On simulating flow with multiple time scales using a method of averages

    Energy Technology Data Exchange (ETDEWEB)

    Margolin, L.G. [Los Alamos National Lab., NM (United States)


    The author presents a new computational method based on averaging to efficiently simulate certain systems with multiple time scales. He first develops the method in a simple one-dimensional setting and employs linear stability analysis to demonstrate numerical stability. He then extends the method to multidimensional fluid flow. His method of averages does not depend on explicit splitting of the equations nor on modal decomposition. Rather he combines low order and high order algorithms in a generalized predictor-corrector framework. He illustrates the methodology in the context of a shallow fluid approximation to an ocean basin circulation. He finds that his new method reproduces the accuracy of a fully explicit second-order accurate scheme, while costing less than a first-order accurate scheme.

  14. Watching the grin fade: tracing the effects of polyploidy on different evolutionary time scales. (United States)

    Mayfield-Jones, Dustin; Washburn, Jacob D; Arias, Tatiana; Edger, Patrick P; Pires, J Chris; Conant, Gavin C


    Polyploidy, or whole-genome duplication (WGD), is a recurrent mutation both in cell lineages and over evolutionary time. By globally changing the relationship between gene copy number and other cellular entities, it can induce dramatic changes at the cellular and phenotypic level. Perhaps surprisingly, then, the insights that these events can bring to understanding other cellular features are not as well appreciated as they could be. In this review, we draw on examples of polyploidy from animals, plants and yeast to explore how investigations of polyploid cells have improved our understanding of the cell cycle, biological network complexity, metabolic phenotypes and tumor biology. We argue that the study of polyploidy across organisms, cell types, and time scales serves not only as a window into basic cell biology, but also as a basis for a predictive biology with applications ranging from crop improvement to treating cancer.

  15. Quantum physical states for describing photonic assisted chemical change: I. Torsional phenomenon at femtosecond time scale

    CERN Document Server

    Tapia, O


    Femtosecond torsional relaxation processes experimentally detected and recently reported by Clark et al. (Nature Phys. 8,225 (2012)) are theoretically dissected with a Hilbert/Fock quantum physical (QP) framework incorporating entanglement of photon/matter base states overcoming standard semi-classic vibrational descriptions. The quantum analysis of a generic Z/E (cis/trans) isomerization in abstract QP terms shed light to fundamental roles played by photonic spin and excited electronic singlet coupled to triplet states. It is shown that one photon activation cannot elicit femtosecond phenomenon, while a two-photon pulse would do. Estimated time scales for the two-photon case indicate the process to lie between a slower than electronic Franck-Condon-like transition yet faster than (semi-classic) vibration relaxation ones.

  16. A Mechanism for Stabilization of Dynamics in Nonlinear Systems with Different Time Scales

    CERN Document Server

    Lopez, Raquel M; Camacho, Erika T


    There are many natural, physical, and biological systems that exhibit multiple time scales. For example, the dynamics of a population of ticks can be described in continuous time during their individual life cycle yet discrete time is used to describe the generation of offspring. These characteristics cause the population levels to be reset periodically. A similar phenomenon can be observed in a sociological college drinking model in which the population is reset by the incoming class each year, as described in the 2006 work of Camacho et al. With the latter as our motivation we analytically and numerically investigate the mechanism by which solutions in certain systems with this resetting conditions stabilize. We further utilize the sociological college drinking model as an analogue to analyze certain one-dimensional and two-dimensional nonlinear systems, as we attempt to generalize our results to higher dimensions.

  17. Attractors of relaxation discrete-time systems with chaotic dynamics on a fast time scale. (United States)

    Maslennikov, Oleg V; Nekorkin, Vladimir I


    In this work, a new type of relaxation systems is considered. Their prominent feature is that they comprise two distinct epochs, one is slow regular motion and another is fast chaotic motion. Unlike traditionally studied slow-fast systems that have smooth manifolds of slow motions in the phase space and fast trajectories between them, in this new type one observes, apart the same geometric objects, areas of transient chaos. Alternating periods of slow regular motions and fast chaotic ones as well as transitions between them result in a specific chaotic attractor with chaos on a fast time scale. We formulate basic properties of such attractors in the framework of discrete-time systems and consider several examples. Finally, we provide an important application of such systems, the neuronal electrical activity in the form of chaotic spike-burst oscillations.

  18. Time-scale characteristics of Kasai river hydrological regime variability for 1940-1999

    CERN Document Server

    Mbuebue, Jean-Marie Tshitenge; Mwamba, Vincent Lukanda; Phuati, Edmond Phuku; Bantu, Albert Kazadi Mukenga; Keto, Franck Tondozi


    The present study was undertaken with the aim of contributing to the characterization of the nonstationary variability of the hydrological regime of the Kasai River using the wavelet analysis for 1940-1999. The rainfalls and discharge over Kasai Basin have marked fluctuations with a perceptible downward trend and some shift around 1950, 1960, 1970, 1983 and 1994. The results show that rainfalls over Kasai basin and the discharge at Ilebo station patterns exhibit a strong annual oscillation and some intermittent oscillations in 2-8 years (1950-1975, 1983-1995) and 8-16 years (1970-1999) time scales. The wavelet coherence analysis reveals a weak possible connection between hydrological variables (rainfalls, discharge) and climate indices relative to sea surface temperature and atmospheric circulation over Atlantic tropical, Indian and Pacific Oceans (coherence less than 0.55).

  19. Application of intrinsic time-scale decomposition (ITD) to EEG signals for automated seizure prediction. (United States)

    Martis, Roshan Joy; Acharya, U Rajendra; Tan, Jen Hong; Petznick, Andrea; Tong, Louis; Chua, Chua Kuang; Ng, Eddie Yin Kwee


    Intrinsic time-scale decomposition (ITD) is a new nonlinear method of time-frequency representation which can decipher the minute changes in the nonlinear EEG signals. In this work, we have automatically classified normal, interictal and ictal EEG signals using the features derived from the ITD representation. The energy, fractal dimension and sample entropy features computed on ITD representation coupled with decision tree classifier has yielded an average classification accuracy of 95.67%, sensitivity and specificity of 99% and 99.5%, respectively using 10-fold cross validation scheme. With application of the nonlinear ITD representation, along with conceptual advancement and improvement of the accuracy, the developed system is clinically ready for mass screening in resource constrained and emerging economy scenarios.

  20. Origin of space-separated charges in photoexcited organic heterojunctions on ultrafast time scales

    CERN Document Server

    Janković, Veljko


    We present detailed investigation of ultrafast (sub-ps) exciton dynamics in the lattice model of a donor/acceptor heterojunction. Exciton generation by means of a photoexcitation, exciton dissociation, and further charge separation are treated on equal footing. The experimentally observed presence of space-separated charges at $\\lesssim 100$ fs after the photoexcitation is usually attributed to ultrafast transitions from excitons in the donor to charge transfer and charge separated states. Here, we show, however, that space-separated charges appearing on $\\lesssim 100$-fs time scales are predominantly directly optically generated. Our theoretical insights into the ultrafast pump-probe spectroscopy challenge usual interpretations of pump-probe spectra in terms of ultrafast population transfer from donor excitons to space-separated charges.

  1. Small quantum absorption refrigerator in the transient regime: Time scales, enhanced cooling, and entanglement. (United States)

    Brask, Jonatan Bohr; Brunner, Nicolas


    A small quantum absorption refrigerator, consisting of three qubits, is discussed in the transient regime. We discuss time scales for coherent dynamics, damping, and approach to the steady state, and we study cooling and entanglement. We observe that cooling can be enhanced in the transient regime, in the sense that lower temperatures can be achieved compared to the steady-state regime. This is a consequence of coherent dynamics but can occur even when this dynamics is strongly damped by the dissipative thermal environment, and we note that precise control over couplings or timing is not needed to achieve enhanced cooling. We also show that the amount of entanglement present in the refrigerator can be much larger in the transient regime compared to the steady state. These results are of relevance to future implementations of quantum thermal machines.

  2. Evidence of Invariance of Time Scale at Critical Point in Ising Meanfield Equilibrium Equation of State

    Institute of Scientific and Technical Information of China (English)

    Muktish Acharyya; Ajanta Bhowal Acharyya


    We solve the equilibrium meanfield equation of state of Ising ferromagnet (obtained from Bragg-Williams theory) by Newton-Raphson method.The number of iterations required to get a convergent solution (within a specified accuracy) of equilibrium magnetisation, at any particular temperature, is observed to diverge in a power law fashion as the temperature approaches the critical value.This is identified as the critical slowing down.The exponent is also estimated.This value of the exponent is compared with that obtained from analytic solution.Besides this, the numerical results are also compared with some experimental results exhibiting satisfactory degree of agreement.It is observed from this study that the information of the invariance of time scale at the critical point is present in the meanfield equilibrium equation of state of Ising ferromagnet.

  3. Mixed-Mode Oscillations in a piecewise linear system with multiple time scale coupling (United States)

    Fernández-García, S.; Krupa, M.; Clément, F.


    In this work, we analyze a four dimensional slow-fast piecewise linear system with three time scales presenting Mixed-Mode Oscillations. The system possesses an attractive limit cycle along which oscillations of three different amplitudes and frequencies can appear, namely, small oscillations, pulses (medium amplitude) and one surge (largest amplitude). In addition to proving the existence and attractiveness of the limit cycle, we focus our attention on the canard phenomena underlying the changes in the number of small oscillations and pulses. We analyze locally the existence of secondary canards leading to the addition or subtraction of one small oscillation and describe how this change is globally compensated for or not with the addition or subtraction of one pulse.

  4. Experimental investigation of atomic collisions in time scales varying from nanosecond to microseconds

    Energy Technology Data Exchange (ETDEWEB)

    Glover, R D; Laban, D E; Matherson, K J; Wallace, W; Sang, R T, E-mail: [Centre for Quantum Dynamics, Griffith University, Nathan, Queensland 4111 (Australia)


    We present the results from two experiments investigating collisions that differ in time scale by three orders of magnitude. The first experiment enables the determination of absolute total collision cross sections using a technique that measures a change in the loss rate of trapped atoms from a magneto optical trap (MOT). We also investigate light assisted collision processes between cold metastable neon atoms in the {sup 3}P{sub 2} metastable state within the MOT. A catalysis laser is scanned in frequency across the {sup 3}P{sub 2} - {sup 3}D{sub 3} cooling transition and the ionization rate was observed. Ionization spectra are obtained which demonstrate a dependence on the magnetic sublevels of the transition that the catalysis laser is exciting.

  5. Wind speed forecasting at different time scales: a non parametric approach

    CERN Document Server

    D'Amico, Guglielmo; Prattico, Flavio


    The prediction of wind speed is one of the most important aspects when dealing with renewable energy. In this paper we show a new nonparametric model, based on semi-Markov chains, to predict wind speed. Particularly we use an indexed semi-Markov model, that reproduces accurately the statistical behavior of wind speed, to forecast wind speed one step ahead for different time scales and for very long time horizon maintaining the goodness of prediction. In order to check the main features of the model we show, as indicator of goodness, the root mean square error between real data and predicted ones and we compare our forecasting results with those of a persistence model.

  6. Optimal feedback scheme and universal time scaling for Hamiltonian parameter estimation. (United States)

    Yuan, Haidong; Fung, Chi-Hang Fred


    Time is a valuable resource and it is expected that a longer time period should lead to better precision in Hamiltonian parameter estimation. However, recent studies in quantum metrology have shown that in certain cases more time may even lead to worse estimations, which puts this intuition into question. In this Letter we show that by including feedback controls this intuition can be restored. By deriving asymptotically optimal feedback controls we quantify the maximal improvement feedback controls can provide in Hamiltonian parameter estimation and show a universal time scaling for the precision limit under the optimal feedback scheme. Our study reveals an intriguing connection between noncommutativity in the dynamics and the gain of feedback controls in Hamiltonian parameter estimation.

  7. Modeling and Control of a Large Nuclear Reactor A Three-Time-Scale Approach

    CERN Document Server

    Shimjith, S R; Bandyopadhyay, B


    Control analysis and design of large nuclear reactors requires a suitable mathematical model representing the steady state and dynamic behavior of the reactor with reasonable accuracy. This task is, however, quite challenging because of several complex dynamic phenomena existing in a reactor. Quite often, the models developed would be of prohibitively large order, non-linear and of complex structure not readily amenable for control studies. Moreover, the existence of simultaneously occurring dynamic variations at different speeds makes the mathematical model susceptible to numerical ill-conditioning, inhibiting direct application of standard control techniques. This monograph introduces a technique for mathematical modeling of large nuclear reactors in the framework of multi-point kinetics, to obtain a comparatively smaller order model in standard state space form thus overcoming these difficulties. It further brings in innovative methods for controller design for systems exhibiting multi-time-scale property,...

  8. Time scales for formation and spreading of velocity shells of pickup ions in the solar wind (United States)

    Gaffey, J. D., Jr.; Wu, C. S.; Winske, D.


    This paper discusses the process of assimilation (pickup) by the solar wind of newly ionized atoms and molecules. Generally, the pickup process is considered to evolve in three stages: (1) the initial interaction of newly created ions with the interplanetary magnetic field to form the ring-beam distribution; (2) pitch angle scattering of the ring beam to form a hollow shell; and (3) slower velocity diffusion to form a partially filled-in shell distribution. Using numerical simulations of turbulence such as would occur naturally in the solar wind and such as would be encountered near cometary bow shocks, the processes of shell formation and evolution are studied, and the results are used to estimate the time scales for shell formation and diffusion in several situations of recent observational interest, the interstellar He data obtained by AMPTE and cometary ion pickup distributions obtained by various spacecraft at comets Giacobini-Zinner and Halley.

  9. Lead isotopic studies of lunar soils - Their bearing on the time scale of agglutinate formation (United States)

    Church, S. E.; Tilton, G. R.; Chen, J. H.


    Fines (smaller than 75 microns) and bulk soil were studied to analyze loss of volatile lead; losses of the order of 10% to 30% radiogenic lead during the production of agglutinates are assessed. Lead isotope data from fine-agglutinate pairs are analyzed for information on the time scale of micrometeorite bombardment, from the chords generated by the data in concordia diagrams. Resulting mean lead loss ages were compared to spallogenic gas exposure ages for all samples. Labile parentless radiogenic Pb residing preferentially on or in the fines is viewed as possibly responsible for aberrant lead loss ages. Bulk soils plot above the concordia curve (in a field of excess radiogenic Pb) for all samples with anomalous ages.

  10. Intrinsic dynamics of heart regulatory systems on short time-scales: from experiment to modelling

    CERN Document Server

    Khovanov, I A; McClintock, P V E; Stefanovska, A


    We discuss open problems related to the stochastic modeling of cardiac function. The work is based on an experimental investigation of the dynamics of heart rate variability (HRV) in the absence of respiratory perturbations. We consider first the cardiac control system on short time scales via an analysis of HRV within the framework of a random walk approach. Our experiments show that HRV on timescales of less than a minute takes the form of free diffusion, close to Brownian motion, which can be described as a non-stationary process with stationary increments. Secondly, we consider the inverse problem of modeling the state of the control system so as to reproduce the experimentally observed HRV statistics of. We discuss some simple toy models and identify open problems for the modelling of heart dynamics.

  11. Singular perturbations and time scales in the design of digital flight control systems (United States)

    Naidu, Desineni S.; Price, Douglas B.


    The results are presented of application of the methodology of Singular Perturbations and Time Scales (SPATS) to the control of digital flight systems. A block diagonalization method is described to decouple a full order, two time (slow and fast) scale, discrete control system into reduced order slow and fast subsystems. Basic properties and numerical aspects of the method are discussed. A composite, closed-loop, suboptimal control system is constructed as the sum of the slow and fast optimal feedback controls. The application of this technique to an aircraft model shows close agreement between the exact solutions and the decoupled (or composite) solutions. The main advantage of the method is the considerable reduction in the overall computational requirements for the evaluation of optimal guidance and control laws. The significance of the results is that it can be used for real time, onboard simulation. A brief survey is also presented of digital flight systems.

  12. Time scales of critical events around the Cretaceous-Paleogene boundary. (United States)

    Renne, Paul R; Deino, Alan L; Hilgen, Frederik J; Kuiper, Klaudia F; Mark, Darren F; Mitchell, William S; Morgan, Leah E; Mundil, Roland; Smit, Jan


    Mass extinctions manifest in Earth's geologic record were turning points in biotic evolution. We present (40)Ar/(39)Ar data that establish synchrony between the Cretaceous-Paleogene boundary and associated mass extinctions with the Chicxulub bolide impact to within 32,000 years. Perturbation of the atmospheric carbon cycle at the boundary likely lasted less than 5000 years, exhibiting a recovery time scale two to three orders of magnitude shorter than that of the major ocean basins. Low-diversity mammalian fauna in the western Williston Basin persisted for as little as 20,000 years after the impact. The Chicxulub impact likely triggered a state shift of ecosystems already under near-critical stress.

  13. Existence of positive solutions for semipositone dynamic system on time scales

    Directory of Open Access Journals (Sweden)

    You-Wei Zhang


    Full Text Available In this paper, we study the following semipositone dynamic system on time scales $$displaylines{ -x^{DeltaDelta}(t=f(t,y+p(t, quad tin(0,T_{mathbb{T}},cr -y^{DeltaDelta}(t=g(t,x, quad tin(0,T_{mathbb{T}},cr x(0=x(sigma^{2}(T=0, cr alpha{y(0}-eta{y^{Delta}{(0}}= gamma{y(sigma(T}+delta{y^{Delta}(sigma(T}=0. }$$ Using fixed point index theory, we show the existence of at least one positive solution. The interesting point is the that nonlinear term is allowed to change sign and may tend to negative infinity.

  14. Recurrence CFD - a novel approach to simulate multiphase flows with strongly separated time scales

    CERN Document Server

    Lichtenegger, Thomas


    Classical Computational Fluid Dynamics (CFD) of long-time processes with strongly separated time scales is computationally extremely demanding if not impossible. Consequently, the state-of-the-art description of such systems is not capable of real-time simulations or online process monitoring. In order to bridge this gap, we propose a new method suitable to decouple slow from fast degrees of freedom in many cases. Based on the recurrence statistics of unsteady flow fields, we deduce a recurrence process which enables the generic representation of pseudo-periodic motion at high spatial and temporal resolution. Based on these fields, passive scalars can be traced by recurrence CFD. While a first, Eulerian Model A solves a passive transport equation in a classical implicit finite-volume environment, a second, Lagrangian Model B propagates fluid particles obeying a stochastic differential equation explicitly. Finally, this new concept is tested by two multiphase processes - a lab scale oscillating bubble column a...

  15. Climate scenarios for Olkiluoto on a time-scale of 120,000 years

    Energy Technology Data Exchange (ETDEWEB)

    Pimenoff, N.; Venaelaeinen, A.; Jaervinen, H. [Finnish Meteorological Institute, Helsinki (Finland)


    Posiva Oy is planning to dispose of spent nuclear fuel in a repository, to be constructed at a depth of 400 m in the crystalline bedrock at Olkiluoto, Finland. Planning the storage requires careful consideration of many aspects, including an assessment of long-term repository safety. For estimating possible climate states at Olkiluoto on a time-scale of 120,000 years, we analyze climate simulations of an Earth System Model of Intermediate Complexity (CLIMBER-2) coupled with an ice sheet model (SICOPOLIS). The simulations into the future clearly show that the onset of the next glaciation is strongly dependent on the Earth's orbital variations and the atmospheric CO{sub 2} concentration. It is evident that due to global warming, the climate of the next centuries will be warmer and wetter than at present. Most likely, due to global warming and low variations in the Earth's orbit around the sun, the present interglacial will last for at least the next 30,000 years. Further, the future simulations showed that the insolation minima on the Northern Hemisphere 50,000-60,000 and 90,000-100,000 years after the present hold a potential for the onset of the next glaciation. Hence, on a time-scale of 120,000 years, one must take into account climate periods lasting several thousand years having the following features: an interglacial climate, a periglacial climate, a climate with an ice sheet margin near Olkiluoto, a glacial climate with an ice sheet covering Olkiluoto, and a climate with Olkiluoto being depressed below sea level after glaciation due to isostatic depression. Due to the uncertainties related to the evolution of the future climate, it is recommended the simulations into the far future to be used only qualitatively. Quantitative information about glacial climate is achieved from the reconstructions and simulations of the past climate. (orig.)

  16. Sensitivity of the simulated precipitation to changes in convective relaxation time scale

    Directory of Open Access Journals (Sweden)

    S. K. Mishra


    Full Text Available The paper describes the sensitivity of the simulated precipitation to changes in convective relaxation time scale (TAU of Zhang and McFarlane (ZM cumulus parameterization, in NCAR-Community Atmosphere Model version 3 (CAM3. In the default configuration of the model, the prescribed value of TAU, a characteristic time scale with which convective available potential energy (CAPE is removed at an exponential rate by convection, is assumed to be 1 h. However, some recent observational findings suggest that, it is larger by around one order of magnitude. In order to explore the sensitivity of the model simulation to TAU, two model frameworks have been used, namely, aqua-planet and actual-planet configurations. Numerical integrations have been carried out by using different values of TAU, and its effect on simulated precipitation has been analyzed.

    The aqua-planet simulations reveal that when TAU increases, rate of deep convective precipitation (DCP decreases and this leads to an accumulation of convective instability in the atmosphere. Consequently, the moisture content in the lower- and mid- troposphere increases. On the other hand, the shallow convective precipitation (SCP and large-scale precipitation (LSP intensify, predominantly the SCP, and thus capping the accumulation of convective instability in the atmosphere. The total precipitation (TP remains approximately constant, but the proportion of the three components changes significantly, which in turn alters the vertical distribution of total precipitation production. The vertical structure of moist heating changes from a vertically extended profile to a bottom heavy profile, with the increase of TAU. Altitude of the maximum vertical velocity shifts from upper troposphere to lower troposphere. Similar response was seen in the actual-planet simulations. With an increase in TAU from 1 h to 8 h, there was a significant improvement in the simulation of the seasonal mean precipitation. The

  17. Approximate method for stochastic chemical kinetics with two-time scales by chemical Langevin equations (United States)

    Wu, Fuke; Tian, Tianhai; Rawlings, James B.; Yin, George


    The frequently used reduction technique is based on the chemical master equation for stochastic chemical kinetics with two-time scales, which yields the modified stochastic simulation algorithm (SSA). For the chemical reaction processes involving a large number of molecular species and reactions, the collection of slow reactions may still include a large number of molecular species and reactions. Consequently, the SSA is still computationally expensive. Because the chemical Langevin equations (CLEs) can effectively work for a large number of molecular species and reactions, this paper develops a reduction method based on the CLE by the stochastic averaging principle developed in the work of Khasminskii and Yin [SIAM J. Appl. Math. 56, 1766-1793 (1996); ibid. 56, 1794-1819 (1996)] to average out the fast-reacting variables. This reduction method leads to a limit averaging system, which is an approximation of the slow reactions. Because in the stochastic chemical kinetics, the CLE is seen as the approximation of the SSA, the limit averaging system can be treated as the approximation of the slow reactions. As an application, we examine the reduction of computation complexity for the gene regulatory networks with two-time scales driven by intrinsic noise. For linear and nonlinear protein production functions, the simulations show that the sample average (expectation) of the limit averaging system is close to that of the slow-reaction process based on the SSA. It demonstrates that the limit averaging system is an efficient approximation of the slow-reaction process in the sense of the weak convergence.

  18. Streamflow response of a small forested catchment on different time scales

    Directory of Open Access Journals (Sweden)

    A. Zabaleta


    Full Text Available The hydrological response of a catchment to rainfall on different time scales is result of a complex system involving a range of physical processes which may operate simultaneously and have different spatial and temporal influences. This paper presents the analysis of streamflow response of a small humid-temperate catchment (Aixola, 4.8 km2 in the Basque Country on different time scales and discusses the role of the controlling factors. Firstly, daily time series analysis was used to establish a hypothesis on the general functioning of the catchment through the relationship between precipitation and discharge on an annual and multi-annual scale (2003–2008. Second, rainfall-runoff relationships and relationships among several hydrological variables, including catchment antecedent conditions, were explored at the event scale (222 events to check and improve the hypothesis. Finally, the evolution of electrical conductivity (EC during some of the monitored storm events (28 events was examined to identify the time-origin of waters. Quick response of the catchment to almost all the rainfall events as well as a considerable regulation capacity was deduced from the correlation and spectral analyses. These results agree with runoff event scale data analysis; however the event analysis revealed the non-linearity of the system, as antecedent conditions play a significant role in this catchment. Further, analysis at the event scale made possible to clarify factors controlling (precipitation, precipitation intensity and initial discharge the different aspects of the runoff response (runoff coefficient and discharge increase for this catchment. Finally, the evolution of EC of the waters enabled the time origin (event or pre-event waters of the quickflow to be established; specifically, the conductivity showed that pre-event waters usually represent a high percentage of the total discharge during runoff peaks. The importance of soil waters in the

  19. Separation of N-derivatized di- and tri-peptide stereoisomers by micro-liquid chromatography using a quinidine-based monolithic column - Analysis of l-carnosine in dietary supplements. (United States)

    Wang, Qiqin; Sánchez-López, Elena; Han, Hai; Wu, Huihui; Zhu, Peijie; Crommen, Jacques; Marina, Maria Luisa; Jiang, Zhengjin


    In the present study, a new analytical methodology was developed enabling the enantiomeric determination of N-derivatized di- and tri-peptides in dietary supplements using chiral micro-LC on a monolithic column consisting of poly(O-9-[2-(methacryloyloxy)-ethylcarbamoyl]-10,11-dihydroquinidine-co-2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) (poly(MQD-co-HEMA-co-EDMA)). After optimization of the mobile phase conditions, a baseline resolution of the stereoisomers of 24 out of 53 N-derivatized di- and tri-peptides was obtained. 3,5-Dinitrobenzoyl- and 3,5-dichlorobenzoyl-peptide stereoisomers were separated with exceptionally high selectivity and resolution. The monolithic column was then applied to the quantitative analysis of l-carnosine and its enantiomeric impurity in three different commercial dietary supplements. Method validation demonstrated satisfactory results in terms of linearity, precision, selectivity, accuracy and limits of detection and quantification. The determined amounts of l-carnosine in commercial formulations were in agreement with the labeled content for all analyzed samples, and the enantiomeric impurity was found to be below the limit of detection (LOD), showing the potential of the poly(MQD-co-HEMA-co-EDMA) monolithic column as a reliable tool for the quality control of l-carnosine in dietary supplements by micro-LC.


    Institute of Scientific and Technical Information of China (English)


    By different fixed point theorems in cones, sufficient conditions for the existence and multiple existence of positive solutions to a class of second-order multi-point boundary value problem for dynamic equation on time scales are obtained.

  1. Influence of time scale wind speed data on sustainability analysis for irrigating greenhouse crops (United States)

    Díaz Méndez, Rodrigo; García Llaneza, Joaquín; Peillón, Manuel; Perdigones, Alicia; Sanchez, Raul; Tarquis, Ana M.; Garcia, Jose Luis


    Appropriate water supply at crop/farm level, with suitable costs, is becoming more and more important. Energy management is closely related to water supply in this context, being wind energy one of the options to be considered, using wind pumps for irrigation water supply. Therefore, it is important to characterize the wind speed frequency distribution to study the technical feasibility to use its energy for irrigation management purpose. The general objective of this present research is to analyze the impact of time scale recorded wind speed data in the sustainability for tomato (Solanum lycopersicum L.) grown under greenhouse at Cuban conditions using drip irrigation system. For this porpoise, a daily estimation balance between water needs and water availability was used to evaluate the feasibility of the most economic windmill irrigation system. Several factors were included: wind velocity (W, m/s) in function of the time scale averaged, flow supplied by the wind pump as a function of the elevation height (H, m) and daily greenhouse evapotranspiration. Monthly volumes of water required for irrigation (Dr, m3/ha) and in the water tank (Vd, m3), as well as the monthly irrigable area (Ar, ha), were estimated by cumulative deficit water budgeting taking in account these factors. Three-hourly wind velocity (W3h, m/s) data from 1992 till 2008 was available for this study. The original data was grouped in six and twelve hourly data (W6h and W12h respectively) as well as daily data (W24h). For each time scale the daily estimation balance was applied. A comparison of the results points out a need for at least three-hourly data to be used mainly in the months in which mean wind speed are close or below the pumps threshold speed to start-up functioning. References Manuel Esteban Peillon Mesa, Ana Maria Tarquis Alfonso, José Luis García Fernández, and Raúl Sánchez Calvo. The use of wind pumps for irrigating greenhouse tomato crops: a case study in Cuba. Geophysical

  2. Observation of quantum particles on a large space-time scale (United States)

    Landau, L. J.


    A quantum particle observed on a sufficiently large space-time scale can be described by means of classical particle trajectories. The joint distribution for large-scale multiple-time position and momentum measurements on a nonrelativistic quantum particle moving freely in R v is given by straight-line trajectories with probabilities determined by the initial momentum-space wavefunction. For large-scale toroidal and rectangular regions the trajectories are geodesics. In a uniform gravitational field the trajectories are parabolas. A quantum counting process on free particles is also considered and shown to converge in the large-space-time limit to a classical counting process for particles with straight-line trajectories. If the quantum particle interacts weakly with its environment, the classical particle trajectories may undergo random jumps. In the random potential model considered here, the quantum particle evolves according to a reversible unitary one-parameter group describing elastic scattering off static randomly distributed impurities (a quantum Lorentz gas). In the large-space-time weak-coupling limit a classical stochastic process is obtained with probability one and describes a classical particle moving with constant speed in straight lines between random jumps in direction. The process depends only on the ensemble value of the covariance of the random field and not on the sample field. The probability density in phase space associated with the classical stochastic process satisfies the linear Boltzmann equation for the classical Lorentz gas, which, in the limit h→0, goes over to the linear Landau equation. Our study of the quantum Lorentz gas is based on a perturbative expansion and, as in other studies of this system, the series can be controlled only for small values of the rescaled time and for Gaussian random fields. The discussion of classical particle trajectories for nonrelativistic particles on a macroscopic spacetime scale applies also to

  3. Variability of interconnected wind plants: correlation length and its dependence on variability time scale (United States)

    St. Martin, Clara M.; Lundquist, Julie K.; Handschy, Mark A.


    The variability in wind-generated electricity complicates the integration of this electricity into the electrical grid. This challenge steepens as the percentage of renewably-generated electricity on the grid grows, but variability can be reduced by exploiting geographic diversity: correlations between wind farms decrease as the separation between wind farms increases. But how far is far enough to reduce variability? Grid management requires balancing production on various timescales, and so consideration of correlations reflective of those timescales can guide the appropriate spatial scales of geographic diversity grid integration. To answer ‘how far is far enough,’ we investigate the universal behavior of geographic diversity by exploring wind-speed correlations using three extensive datasets spanning continents, durations and time resolution. First, one year of five-minute wind power generation data from 29 wind farms span 1270 km across Southeastern Australia (Australian Energy Market Operator). Second, 45 years of hourly 10 m wind-speeds from 117 stations span 5000 km across Canada (National Climate Data Archive of Environment Canada). Finally, four years of five-minute wind-speeds from 14 meteorological towers span 350 km of the Northwestern US (Bonneville Power Administration). After removing diurnal cycles and seasonal trends from all datasets, we investigate dependence of correlation length on time scale by digitally high-pass filtering the data on 0.25-2000 h timescales and calculating correlations between sites for each high-pass filter cut-off. Correlations fall to zero with increasing station separation distance, but the characteristic correlation length varies with the high-pass filter applied: the higher the cut-off frequency, the smaller the station separation required to achieve de-correlation. Remarkable similarities between these three datasets reveal behavior that, if universal, could be particularly useful for grid management. For high

  4. Peptidomic Identification of Serum Peptides Diagnosing Preeclampsia.

    Directory of Open Access Journals (Sweden)

    Qiaojun Wen

    Full Text Available We sought to identify serological markers capable of diagnosing preeclampsia (PE. We performed serum peptide analysis (liquid chromatography mass spectrometry of 62 unique samples from 31 PE patients and 31 healthy pregnant controls, with two-thirds used as a training set and the other third as a testing set. Differential serum peptide profiling identified 52 significant serum peptides, and a 19-peptide panel collectively discriminating PE in training sets (n = 21 PE, n = 21 control; specificity = 85.7% and sensitivity = 100% and testing sets (n = 10 PE, n = 10 control; specificity = 80% and sensitivity = 100%. The panel peptides were derived from 6 different protein precursors: 13 from fibrinogen alpha (FGA, 1 from alpha-1-antitrypsin (A1AT, 1 from apolipoprotein L1 (APO-L1, 1 from inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4, 2 from kininogen-1 (KNG1, and 1 from thymosin beta-4 (TMSB4. We concluded that serum peptides can accurately discriminate active PE. Measurement of a 19-peptide panel could be performed quickly and in a quantitative mass spectrometric platform available in clinical laboratories. This serum peptide panel quantification could provide clinical utility in predicting PE or differential diagnosis of PE from confounding chronic hypertension.

  5. Antimicrobial Peptides

    Directory of Open Access Journals (Sweden)

    Ali Adem Bahar


    Full Text Available The rapid increase in drug-resistant infections has presented a serious challenge to antimicrobial therapies. The failure of the most potent antibiotics to kill “superbugs” emphasizes the urgent need to develop other control agents. Here we review the history and new development of antimicrobial peptides (AMPs, a growing class of natural and synthetic peptides with a wide spectrum of targets including viruses, bacteria, fungi, and parasites. We summarize the major types of AMPs, their modes of action, and the common mechanisms of AMP resistance. In addition, we discuss the principles for designing effective AMPs and the potential of using AMPs to control biofilms (multicellular structures of bacteria embedded in extracellular matrixes and persister cells (dormant phenotypic variants of bacterial cells that are highly tolerant to antibiotics.

  6. Strong cation exchange chromatography in analysis of posttranslational modifications: innovations and perspectives. (United States)

    Edelmann, Mariola J


    Strong cation exchange (SCX) chromatography has been utilized as an excellent separation technique that can be combined with reversed-phase (RP) chromatography, which is frequently used in peptide mass spectrometry. Although SCX is valuable as the second component of such two-dimensional separation methods, its application goes far beyond efficient fractionation of complex peptide mixtures. Here I describe how SCX facilitates mapping of the protein posttranslational modifications (PTMs), specifically phosphorylation and N-terminal acetylation. The SCX chromatography has been mainly used for enrichment of these two PTMs, but it might also be beneficial for high-throughput analysis of other modifications that alter the net charge of a peptide.

  7. Evaluating the uncertainty of predicting future climate time series at the hourly time scale (United States)

    Caporali, E.; Fatichi, S.; Ivanov, V. Y.


    A stochastic downscaling methodology is developed to generate hourly, point-scale time series for several meteorological variables, such as precipitation, cloud cover, shortwave radiation, air temperature, relative humidity, wind speed, and atmospheric pressure. The methodology uses multi-model General Circulation Model (GCM) realizations and an hourly weather generator, AWE-GEN. Probabilistic descriptions of factors of change (a measure of climate change with respect to historic conditions) are computed for several climate statistics and different aggregation times using a Bayesian approach that weights the individual GCM contributions. The Monte Carlo method is applied to sample the factors of change from their respective distributions thereby permitting the generation of time series in an ensemble fashion, which reflects the uncertainty of climate projections of future as well as the uncertainty of the downscaling procedure. Applications of the methodology and probabilistic expressions of certainty in reproducing future climates for the periods, 2000 - 2009, 2046 - 2065 and 2081 - 2100, using the 1962 - 1992 period as the baseline, are discussed for the location of Firenze (Italy). The climate predictions for the period of 2000 - 2009 are tested against observations permitting to assess the reliability and uncertainties of the methodology in reproducing statistics of meteorological variables at different time scales.

  8. Southern Ocean SST Variability and Its Relationship with ENSO on Inter-Decadal Time Scales

    Institute of Scientific and Technical Information of China (English)

    YAN Li; DU Yan; ZHANG Lan


    Empirical orthogonal function (EOF) analysis reveals a co-variability of Sea surface temperatures (SSTs) in the Southern Hemisphere (0°-60°S).In the South Indian and Atlantic Oceans,there is a subtropical dipole pattern slanted in the southwest-northeast direction.In the South Pacific Ocean,a meridional tripole structure emerges,whose middle pole co-varies with the dipoles in the South Indian and Atlantic Oceans and is used in this study to track subtropical Pacific variability.The South Indian and Atlantic Ocean dipoles and the subtropical Pacific variability are phase-locked in austral summer.On the inter-decadal time scales,the dipoles in the South Indian and Atlantic Oceans weaken in amplitude after 1979/1980.No such weakening is found in the subtropical South Pacific Ocean.Interestingly,despite the reduced amplitude,the correlation of the Indian Ocean and Atlantic dipoles with El Ni(n)o and Southern Oscillation (ENSO) are enhanced after 1979/1980.The same increase in correlation is found for subtropical South Pacific variability after 1979/1980.These inter-decadal modulations imply that the Southem Hemisphere participates in part of the climate shift in the late 1970s.The correlation between Southern Hemisphere SST and ENSO reduces after 2000.

  9. Application of matched filters in time-scale domain for elastography

    Institute of Scientific and Technical Information of China (English)

    ZHEN Bin; WAN Mingxi; SUN Yongmei


    Elasticity imaging offers the possibility of detecting changes in elastic properties and assesses the biomechanical properties of soft tissue with increased sensitivity and spatial resolution compared with conventional palpation. However, the range of applied strains is limited by the concomitant increase of echo signal decorrelation. The decorrelation is mainly introduced by diffuse scattering, while the regular scattering is highly correlated. Because the regular scattering and diffuse scattering localize with different patterns in different ranges of time-scale plane, a new method is put forward to detect the regular scattering with matched filters based on wavelet transform using Generalized Likelihood Ratio Test (GLRT). The simulation results illustrate that the change in estimated mean interscatterer spacing introduced by a SNR of -10 dB is 1.1 ± 2.8%. Thus, by tracking the highly correlated regular scattering,the internal strain can be estimated based on the change in interscatterer spacing under the condition of large surface deformation. The experiment studies show that the internal strain can be estimated up to 10% applied deformation in phantom and 5% strain in porcine liver.

  10. Time scales of representation in the human brain: weighing past information to predict future events

    Directory of Open Access Journals (Sweden)

    Lee eHarrison


    Full Text Available The estimates that humans make of statistical dependencies in the environment and therefore their representation of uncertainty crucially depend on the integration of data over time. As such, the extent to which past events are used to represent uncertainty has been postulated to vary over the cortex. For example, primary visual cortex responds to rapid perturbations in the environment, while frontal cortices involved in executive control encode the longer term contexts within which these perturbations occur. Here we tested whether primary and executive regions can be distinguished by the number of past observations they represent. This was based on a decay-dependent model that weights past observations from a Markov process and Bayesian Model Selection (BMS to test the prediction that neuronal responses are characterised by different decay half-lives depending on location in the brain. We show distributions of brain responses for short and long term decay functions in primary and secondary visual and frontal cortices, respectively. We found that visual and parietal responses are released from the burden of the past, enabling an agile response to fluctuations in events as they unfold. In contrast, frontal regions are more concerned with average trends over longer time scales within which local variations are embedded. Specifically, we provide evidence for a temporal gradient for representing context within the prefrontal cortex and possibly beyond to include primary sensory and association areas.

  11. Mechanisms and time scales of glacial inception simulated with an Earth system model of intermediate complexity

    Directory of Open Access Journals (Sweden)

    R. Calov


    Full Text Available We investigate glacial inception and glacial thresholds in the climate-cryosphere system utilising the Earth system model of intermediate complexity CLIMBER-2, which includes modules for atmosphere, terrestrial vegetation, ocean and interactive ice sheets. The latter are described by the three-dimensional polythermal ice-sheet model SICOPOLIS. A bifurcation which represents glacial inception is analysed with two different model setups: one setup with dynamical ice-sheet model and another setup without it. The respective glacial thresholds differ in terms of maximum boreal summer insolation at 65° N (hereafter referred as Milankovich forcing (MF. The glacial threshold of the configuration without ice-sheet dynamics corresponds to a much lower value of the MF compared to the full model. If MF attains values only slightly below the aforementioned threshold there is fast transient response. Depending on the value of MF relative to the glacial threshold, the transient response time of inland-ice volume in the model configuration with ice-sheet dynamics ranges from 10 000 to 100 000 years. We investigate implications of these time scales for past glacial inceptions and for the overdue Holocene glaciation hypothesis by Ruddiman (W. F. Ruddiman, Climatic Change 2003, Vol. 61, 261–293. We also have shown that the asynchronous coupling between climate and inland-ice components allows one sufficient realistic simulation of glacial inception and, at the same time, a considerable reduction of computational costs.

  12. High-precision astrometry on the VLT/FORS1 at time scales of few days

    CERN Document Server

    Lazorenko, P F; Dominik, M; Pepe, F; Ségransan, D; Udry, S


    We investigate the accuracy of astrometric measurements with the VLT/FORS1 camera and consider potential applications. The study is based on two-epoch (2000 and 2002/2003) frame series of observations of a selected Galactic Bulge sky region that were obtained with FORS1 during four consecutive nights each. Reductions were carried out with a novel technique that eliminates atmospheric image motion and does not require a distinction between targets and reference objects. The positional astrometric precision was found to be limited only by the accuracy of the determination of the star photocentre, which is typically 200-300 microarcsec per single measurement for bright unsaturated stars B=18-19. Several statistical tests have shown that at time-scales of 1-4 nights the residual noise in measured positions is essentially a white noise with no systematic instrumental signature and no significant deviation from a Gaussian distribution. Some evidence of a good astrometric quality of the VLT for frames separated by t...

  13. Multi-Time Scale Control of Demand Flexibility in Smart Distribution Networks

    Directory of Open Access Journals (Sweden)

    Bishnu P. Bhattarai


    Full Text Available This paper presents a multi-timescale control strategy to deploy electric vehicle (EV demand flexibility for simultaneously providing power balancing, grid congestion management, and economic benefits to participating actors. First, an EV charging problem is investigated from consumer, aggregator, and distribution system operator’s perspectives. A hierarchical control architecture (HCA comprising scheduling, coordinative, and adaptive layers is then designed to realize their coordinative goal. This is realized by integrating multi-time scale controls that work from a day-ahead scheduling up to real-time adaptive control. The performance of the developed method is investigated with high EV penetration in a typical residential distribution grid. The simulation results demonstrate that HCA efficiently utilizes demand flexibility stemming from EVs to solve grid unbalancing and congestions with simultaneous maximization of economic benefits to the participating actors. This is ensured by enabling EV participation in day-ahead, balancing, and regulation markets. For the given network configuration and pricing structure, HCA ensures the EV owners to get paid up to five times the cost they were paying without control.

  14. Saharan dust deposition may affect phytoplankton growth in the Mediterranean sea at ecological time scales.

    Directory of Open Access Journals (Sweden)

    Rachele Gallisai

    Full Text Available The surface waters of the Mediterranean Sea are extremely poor in the nutrients necessary for plankton growth. At the same time, the Mediterranean Sea borders with the largest and most active desert areas in the world and the atmosphere over the basin is subject to frequent injections of mineral dust particles. We describe statistical correlations between dust deposition over the Mediterranean Sea and surface chlorophyll concentrations at ecological time scales. Aerosol deposition of Saharan origin may explain 1 to 10% (average 5% of seasonally detrended chlorophyll variability in the low nutrient-low chlorophyll Mediterranean. Most of the statistically significant correlations are positive with main effects in spring over the Eastern and Central Mediterranean, conforming to a view of dust events fueling needed nutrients to the planktonic community. Some areas show negative effects of dust deposition on chlorophyll, coinciding with regions under a large influence of aerosols from European origin. The influence of dust deposition on chlorophyll dynamics may become larger in future scenarios of increased aridity and shallowing of the mixed layer.

  15. Asymptotic self-similar solutions with a characteristic time-scale

    CERN Document Server

    Waxman, Eli


    For a wide variety of initial and boundary conditions, adiabatic one dimensional flows of an ideal gas approach self-similar behavior when the characteristic length scale over which the flow takes place, $R$, diverges or tends to zero. It is commonly assumed that self-similarity is approached since in the $R\\to\\infty(0)$ limit the flow becomes independent of any characteristic length or time scales. In this case the flow fields $f(r,t)$ must be of the form $f(r,t)=t^{\\alpha_f}F(r/R)$ with $R\\propto(\\pm t)^\\alpha$. We show that requiring the asymptotic flow to be independent only of characteristic length scales imply a more general form of self-similar solutions, $f(r,t)=R^{\\delta_f}F(r/R)$ with $\\dot{R}\\propto R^\\delta$, which includes the exponential ($\\delta=1$) solutions, $R\\propto e^{t/\\tau}$. We demonstrate that the latter, less restrictive, requirement is the physically relevant one by showing that the asymptotic behavior of accelerating blast-waves, driven by the release of energy at the center of a co...

  16. A SKOS-based multilingual thesaurus of geological time scale for interoperability of online geological maps (United States)

    Ma, Xiaogang; Carranza, Emmanuel John M.; Wu, Chonglong; van der Meer, Freek D.; Liu, Gang


    The usefulness of online geological maps is hindered by linguistic barriers. Multilingual geoscience thesauri alleviate linguistic barriers of geological maps. However, the benefits of multilingual geoscience thesauri for online geological maps are less studied. In this regard, we developed a multilingual thesaurus of geological time scale (GTS) to alleviate linguistic barriers of GTS records among online geological maps. We extended the Simple Knowledge Organization System (SKOS) model to represent the ordinal hierarchical structure of GTS terms. We collected GTS terms in seven languages and encoded them into a thesaurus by using the extended SKOS model. We implemented methods of characteristic-oriented term retrieval in JavaScript programs for accessing Web Map Services (WMS), recognizing GTS terms, and making translations. With the developed thesaurus and programs, we set up a pilot system to test recognitions and translations of GTS terms in online geological maps. Results of this pilot system proved the accuracy of the developed thesaurus and the functionality of the developed programs. Therefore, with proper deployments, SKOS-based multilingual geoscience thesauri can be functional for alleviating linguistic barriers among online geological maps and, thus, improving their interoperability.

  17. Genome-Wide Motif Statistics are Shaped by DNA Binding Proteins over Evolutionary Time Scales (United States)

    Qian, Long; Kussell, Edo


    The composition of a genome with respect to all possible short DNA motifs impacts the ability of DNA binding proteins to locate and bind their target sites. Since nonfunctional DNA binding can be detrimental to cellular functions and ultimately to organismal fitness, organisms could benefit from reducing the number of nonfunctional DNA binding sites genome wide. Using in vitro measurements of binding affinities for a large collection of DNA binding proteins, in multiple species, we detect a significant global avoidance of weak binding sites in genomes. We demonstrate that the underlying evolutionary process leaves a distinct genomic hallmark in that similar words have correlated frequencies, a signal that we detect in all species across domains of life. We consider the possibility that natural selection against weak binding sites contributes to this process, and using an evolutionary model we show that the strength of selection needed to maintain global word compositions is on the order of point mutation rates. Likewise, we show that evolutionary mechanisms based on interference of protein-DNA binding with replication and mutational repair processes could yield similar results and operate with similar rates. On the basis of these modeling and bioinformatic results, we conclude that genome-wide word compositions have been molded by DNA binding proteins acting through tiny evolutionary steps over time scales spanning millions of generations.

  18. Modeling and simulation of nuclear fuel in scenarios with long time scales

    Energy Technology Data Exchange (ETDEWEB)

    Espinosa, Carlos E.; Bodmann, Bardo E.J., E-mail:, E-mail: [Universidade Federal do Rio Grande do Sul (DENUC/PROMEC/UFRGS), Porto Alegre, RS (Brazil). Departamento de Engenharia Nuclear. Programa de Pos Graduacao em Engenharia Mecanica


    Nuclear reactors play a key role in defining the energy matrix. A study by the Fraunhofer Society shows in different time scales for long periods of time the distribution of energy sources. Regardless of scale, the use of nuclear energy is practically constant. In these scenarios, the nuclear fuel behavior over time is of interest. For kinetics of long-term scales, changing the chemical composition of fuel is significant. Thus, it is appropriate to consider fission products called neutron poisons. Such products are of interest in the nuclear reactor, since they become parasitic neutron absorbers and result in long thermal heat sources. The objective of this work is to solve the kinetics system coupled to neutron poison products. To solve this system, we use similar ideas to the method of Adomian decomposition. Initially, one separates the system of equations as the sum of a linear part and a non-linear part in order to solve a recursive system. The nonlinearity is treated as Adomian polynomial. We present numerical results of the effects of changing the power of a reactor, scenarios such as start-up and shut-down. For these results we consider time dependent reactivity, such as linear reactivity, quadratic polynomial and oscillatory. With these results one can simulate the chemical composition of the fuel due to the reuse of the spent fuel in subsequent cycles. (author)

  19. Multiplicity results for a generalized Sturm-Liouville dynamical system on time scales

    Directory of Open Access Journals (Sweden)

    Zhang Youwei


    Full Text Available Abstract By applying the fixed point theorem in cones, some new and general results on the existence of positive solution to second order generalized Sturm-Liouville dynamical system on time scale T u 1 Δ Δ ( t + h 1 ( t f 1 ( t , u 1 ( t , u 2 ( t , u 1 Δ ( t , u 2 Δ ( t = 0 , u 2 Δ Δ ( t + h 2 ( t f 2 ( t , u 1 ( t , u 2 ( t , u 1 Δ ( t , u 2 Δ ( t = 0 , t ∈ [ t 1 , t 2 ] T , a u i ( t 1 - b u i Δ ( t 1 = ∑ k = 1 m - 2 a k u i ( ξ k , c u i ( σ ( t 2 + d u i Δ ( σ ( t 2 = ∑ k = 1 m - 2 b k u i ( ξ k , ( i = 1 , 2 , are obtained. The first-order Δ-derivatives are involved in the nonlinear terms explicitly. Mathematics Subject Classification (2000 39A10


    Institute of Scientific and Technical Information of China (English)

    Al-Moussawy Raed; Yin Junxun; Song Shaopeng


    This work is concerned with the development and optimization of a signal model for scalable perceptual audio coding at low bit rates. A complementary two-part signal model consisting of Sines plus Noise (SN) is described. The paper presents essentially a fundamental enhancement to the sinusoidal modeling component. The enhancement involves an audio signal scheme based on carrying out overlap-add sinusoidal modeling at three successive time scales,large, medium, and small. The sinusoidal modeling is done in an analysis-by-synthesis overlapadd manner across the three scales by using a psychoacoustically weighted matching pursuits.The sinusoidal modeling residual at the first scale is passed to the smaller scales to allow for the modeling of various signal features at appropriate resolutions. This approach greatly helps to correct the pre-echo inherent in the sinusoidal model. This improves the perceptual audio quality upon our previous work of sinusoidal modeling while using the same number of sinusoids. The most obvious application for the SN model is in scalable, high fidelity audio coding and signal modification.

  1. Multi-resolution schemes for time scaled propagation of wave packets

    CERN Document Server

    Frapiccini, Ana Laura; Mota-Furtado, Francisca; O'Mahony, Patrick F; Piraux, Bernard


    We present a detailed analysis of the time scaled coordinate approach and its implementation for solving the time-dependent Schr\\"odinger equation describing the interaction of atoms or molecules with radiation pulses. We investigate and discuss the performance of multi-resolution schemes for the treatment of the squeezing around the origin of the bound part of the scaled wave packet. When the wave packet is expressed in terms of B-splines, we consider two different types of breakpoint sequences: an exponential sequence with a constant density and an initially uniform sequence with a density of points around the origin that increases with time. These two multi-resolution schemes are tested in the case of a one-dimensional gaussian potential and for atomic hydrogen. In the latter case, we also use Sturmian functions to describe the scaled wave packet and discuss a multi-resolution scheme which consists in working in a sturmian basis characterized by a set of non-linear parameters. Regarding the continuum part ...

  2. The time scales of the climate-economy feedback and the climatic cost of growth

    Energy Technology Data Exchange (ETDEWEB)

    Hallegatte, Stephane [CIRED - CNRM, Nogent-sur-Marne (France)


    This paper is based on the perception that the inertia of climate and socio-economic systems are key parameters in the climate change issue. In a first part, it develops and implements a new approach based on a simple integrated model with a particular focus on an innovative transient impact and adaptation modelling. In a second part, a climate-economy feedback is defined and characterized. It is found that: (i) it has a 70-year characteristic time, which is long when compared to the system's other time-scales, and it cannot act as a natural damping process of climate change; (ii) mitigation has to be anticipated since the feedback of an emission reduction on the economy is significant only after a 20-year delay and really efficient after a one-century delay; (iii) the IPCC methodology, that neglects the feedback from impacts to emissions, is acceptable up to 2100, whatever is the level of impacts. This analysis allows also to define a climatic cost of growth as the additional climate change damages due to the additional emissions linked to economic growth. Usefully, this metric for climate change damages is particularly independent of the baseline scenario. (orig.)

  3. Time scales of regional circulation of saline fluids in continental aquifers (Armorican massif, Western France

    Directory of Open Access Journals (Sweden)

    A. Armandine Les Landes


    Full Text Available In recent decades, saline fluids have been sampled worldwide at great depths in continental basements. Although some of them have been attributed to marine transgressions the mechanisms allowing their circulation is not understood. In this paper, we describe the horizontal and vertical distribution of moderately saline fluids (60 to 1400 mg L−1 sampled at depths ranging from 41 to 200 m in aquifers at the regional scale of the Armorican Massif (northwestern France. The horizontal and vertical distributions of high chloride concentrations are in good agreement with both the altitudinal and vertical limits and succession of the three major transgressions between the Mio-Pliocene and Pleistocene ages. The mean chloride concentration for each transgression area is exponentially related to the time spanned until present. It defines the potential laws of leaching of marine waters by fresh meteoric waters. The results of the Armorican aquifers provide the first observed constraints for the time scales of seawater circulation in the continental basement and the subsequent leaching by fresh meteoric waters. The general trend of increasing chloride concentration with depth and the time frame for the flushing process provide useful information to develop conceptual models of the paleo-functionning of Armorican aquifers.

  4. Minimum spanning tree filtering of correlations for varying time scales and size of fluctuations

    CERN Document Server

    Kwapien, Jaroslaw; Forczek, Marcin; Drozdz, Stanislaw


    Based on a recently proposed $q$-dependent detrended cross-correlation coefficient $\\rho_q$ (J.~Kwapie\\'n, P.~O\\'swi\\k{e}cimka, S.~Dro\\.zd\\.z, Phys. Rev.~E 92, 052815 (2015)), we introduce a family of $q$-dependent minimum spanning trees ($q$MST) that are selective to cross-correlations between different fluctuation amplitudes and different time scales of multivariate data. They inherit this ability directly from the coefficients $\\rho_q$ that are processed here to construct a distance matrix being the input to the MST-constructing Kruskal's algorithm. In order to illustrate their performance, we apply the $q$MSTs to sample empirical data from the American stock market and discuss the results. We show that the $q$MST graphs can complement $\\rho_q$ in detection of "hidden" correlations that cannot be observed by the MST graphs based on $\\rho_{\\rm DCCA}$ and, therefore, they can be useful in many areas where the multivariate cross-correlations are of interest (e.g., in portfolio analysis).

  5. Characterization of discontinuities in the Stripa granite. Time-scale heater experiment

    Energy Technology Data Exchange (ETDEWEB)

    Thorpe, R.


    The report describes the methodology and results of a detailed study of geologic discontinuities associated with the time-scale heater test at the Stripa mine in Sweden. Mapping of the floor of the experiment tunnel coupled with observation of core samples from beneath the drift indicate that four N-striking shear fractures dip steeply through the 6 x 10 x 25-m rock mass. Oblique-thrust faulting has produced displacements of up to 2 m on one of the surfaces, and its inferred 3-D configuration is consistent with observed slickensiding. Resolution of locally measured principal stresses on the shear plane yields a theoretical shear direction that also coincides with the slickensiding. Four distinct joint sets exist locally, one of which coincides with the shear fractures. Another lies nearly horizontal, and two others are steeply inclined. Fracture length and spacing distributions for the four joint sets are shown to be lognormal. Two of the sets lie perpendicular to principal stress directions. The fact that one of these two joint sets apparently post-dates other fracturing and is normal to the minimum principal stress suggests that it is due to isostatic rebound.

  6. Multiple time scales in cataclysmic binaries. The low-field magnetic dwarf nova DO Draconis

    CERN Document Server

    Andronov, I L; Han, W; Kim, Y; Yoon, J -N


    We study the variability of the cataclysmic variable DO Dra, on time-scales of between minutes and decades. The characteristic decay time dt/dm=0.902(3) days/mag was estimated from our 3 nights of CCD R observations. The quiescent data show a photometric wave with a cycle about 303(15)d. We analyzed the profile of the composite (or mean) outburst. We discovered however, that a variety of different outburst heights and durations had occurred, contrary to theoretical predictions. With increasing maximum brightness, we find that the decay time also increases; this is in contrast to the model predictions, which indicate that outbursts should have a constant shape. This is interpreted as representing the presence of outburst-to-outburst variability of the magnetospheric radius. A presence of a number of missed weak narrow outbursts is predicted from this statistical relationship. A new type of variability is detected, during 3 subsequent nights in 2007: periodic (during one nightly run) oscillations with rapidly-d...

  7. Satellite quenching time-scales in clusters from projected phase space measurements matched to simulated orbits (United States)

    Oman, Kyle A.; Hudson, Michael J.


    We measure the star formation quenching efficiency and time-scale in cluster environments. Our method uses N-body simulations to estimate the probability distribution of possible orbits for a sample of observed Sloan Digital Sky Survey galaxies in and around clusters based on their position and velocity offsets from their host cluster. We study the relationship between their star formation rates and their likely orbital histories via a simple model in which star formation is quenched once a delay time after infall has elapsed. Our orbit library method is designed to isolate the environmental effect on the star formation rate due to a galaxy's present-day host cluster from `pre-processing' in previous group hosts. We find that quenching of satellite galaxies of all stellar masses in our sample (109-10^{11.5}M_{⊙}) by massive (> 10^{13} M_{⊙}) clusters is essentially 100 per cent efficient. Our fits show that all galaxies quench on their first infall, approximately at or within a Gyr of their first pericentric passage. There is little variation in the onset of quenching from galaxy-to-galaxy: the spread in this time is at most ˜2 Gyr at fixed M*. Higher mass satellites quench earlier, with very little dependence on host cluster mass in the range probed by our sample.

  8. A study of photothermal laser ablation of various polymers on microsecond time scales. (United States)

    Kappes, Ralf S; Schönfeld, Friedhelm; Li, Chen; Golriz, Ali A; Nagel, Matthias; Lippert, Thomas; Butt, Hans-Jürgen; Gutmann, Jochen S


    To analyze the photothermal ablation of polymers, we designed a temperature measurement setup based on spectral pyrometry. The setup allows to acquire 2D temperature distributions with 1 μm size and 1 μs time resolution and therefore the determination of the center temperature of a laser heating process. Finite element simulations were used to verify and understand the heat conversion and heat flow in the process. With this setup, the photothermal ablation of polystyrene, poly(α-methylstyrene), a polyimide and a triazene polymer was investigated. The thermal stability, the glass transition temperature Tg and the viscosity above Tg were governing the ablation process. Thermal decomposition for the applied laser pulse of about 10 μs started at temperatures similar to the start of decomposition in thermogravimetry. Furthermore, for polystyrene and poly(α-methylstyrene), both with a Tg in the range between room and decomposition temperature, ablation already occurred at temperatures well below the decomposition temperature, only at 30-40 K above Tg. The mechanism was photomechanical, i.e. a stress due to the thermal expansion of the polymer was responsible for ablation. Low molecular weight polymers showed differences in photomechanical ablation, corresponding to their lower Tg and lower viscosity above the glass transition. However, the difference in ablated volume was only significant at higher temperatures in the temperature regime for thermal decomposition at quasi-equilibrium time scales.

  9. Mastering Uncertainty and Risk at Multiple Time Scales in the Future Electrical Grid

    Energy Technology Data Exchange (ETDEWEB)

    Chertkov, Michael [Los Alamos National Laboratory; Bent, Russell W. [Los Alamos National Laboratory; Backhaus, Scott N. [Los Alamos National Laboratory


    Today's electrical grids enjoy a relatively clean separation of spatio-temporal scales yielding a compartmentalization of grid design, optimization, control and risk assessment allowing for the use of conventional mathematical tools within each area. In contrast, the future grid will incorporate time-intermittent renewable generation, operate via faster electrical markets, and tap the latent control capability at finer grid modeling scales; creating a fundamentally new set of couplings across spatiotemporal scales and requiring revolutionary advances in mathematics techniques to bridge these scales. One example is found in decade-scale grid expansion planning in which today's algorithms assume accurate load forecasts and well-controlled generation. Incorporating intermittent renewable generation creates fluctuating network flows at the hourly time scale, inherently linking the ability of a transmission line to deliver electrical power to hourly operational decisions. New operations-based planning algorithms are required, creating new mathematical challenges. Spatio-temporal scales are also crossed when the future grid's minute-scale fluctuations in network flows (due to intermittent generation) create a disordered state upon which second-scale transient grid dynamics propagate effectively invalidating today's on-line dynamic stability analyses. Addressing this challenge requires new on-line algorithms that use large data streams from new grid sensing technologies to physically aggregate across many spatial scales to create responsive, data-driven dynamic models. Here, we sketch the mathematical foundations of these problems and potential solutions.

  10. Mixed-mode oscillations in a multiple time scale phantom bursting system

    CERN Document Server

    Krupa, Maciej; Desroches, Mathieu; Clément, Frédérique


    In this work we study mixed mode oscillations in a model of secretion of GnRH (Gonadotropin Releasing Hormone). The model is a phantom burster consisting of two feedforward coupled FitzHugh-Nagumo systems, with three time scales. The forcing system (Regulator) evolves on the slowest scale and acts by moving the slow nullcline of the forced system (Secretor). There are three modes of dynamics: pulsatility (transient relaxation oscillation), surge (quasi steady state) and small oscillations related to the passage of the slow nullcline through a fold point of the fast nullcline. We derive a variety of reductions, taking advantage of the mentioned features of the system. We obtain two results; one on the local dynamics near the fold in the parameter regime corresponding to the presence of small oscillations and the other on the global dynamics, more specifically on the existence of an attracting limit cycle. Our local result is a rigorous characterization of small canards and sectors of rotation in the case of fo...

  11. Field Experience with and Potential for Multi-time Scale Grid Transactions from Responsive Commercial Buildings

    Energy Technology Data Exchange (ETDEWEB)

    Piette, Mary Ann; Kiliccote, Sila; Ghatikar, Girish


    The need for and concepts behind demand response are evolving. As the electric system changes with more intermittent renewable electric supply systems, there is a need to allow buildings to provide more flexible demand. This paper presents results from field studies and pilots, as well as engineering estimates of the potential capabilities of fast load responsiveness in commercial buildings. We present a sector wide analysis of flexible loads in commercial buildings, which was conducted to improve resource planning and determine which loads to evaluate in future demonstrations. These systems provide important capabilities for future transactional systems. The field analysis is based on results from California, plus projects in the northwest and east coast. End-uses considered include heating, ventilation, air conditioning and lighting. The timescales of control include day-ahead, as well as day-of, 10-minute ahead and even faster response. This technology can provide DR signals on different times scales to interact with responsive building loads. We describe the latency of the control systems in the building and the round trip communications with the wholesale grid operators.

  12. Peptide arrays for screening cancer specific peptides. (United States)

    Ahmed, Sahar; Mathews, Anu Stella; Byeon, Nara; Lavasanifar, Afsaneh; Kaur, Kamaljit


    In this paper, we describe a novel method to screen peptides for specific recognition by cancer cells. Seventy peptides were synthesized on a cellulose membrane in an array format, and a direct method to study the peptide-whole cell interaction was developed. The relative binding affinity of the cells for different peptides with respect to a lead 12-mer p160 peptide, identified by phage display, was evaluated using the CyQUANT fluorescence of the bound cells. Screening allowed identification of at least five new peptides that displayed higher affinity (up to 3-fold) for MDA-MB-435 and MCF-7 human cancer cells compared to the p160 peptide. These peptides showed very little binding to the control (noncancerous) human umbilical vein endothelial cells (HUVECs). Three of these peptides were synthesized separately and labeled with fluorescein isothiocyanate (FITC) to study their uptake and interaction with the cancer and control cells using confocal laser scanning microscopy and flow cytometry. The results confirmed the high and specific affinity of an 11-mer peptide 11 (RGDPAYQGRFL) and a 10-mer peptide 18 (WXEAAYQRFL) for the cancer cells versus HUVECs. Peptide 11 binds different receptors on target cancer cells as its sequence contains multiple recognition motifs, whereas peptide 18 binds mainly to the putative p160 receptor. The peptide array-whole cell binding assay reported here is a complementary method to phage display for further screening and optimization of cancer targeting peptides for cancer therapy and diagnosis.

  13. Polymer-Peptide Nanoparticles: Synthesis and Characterization (United States)

    Dong, He; Shu, Jessica Y.; Xu, Ting


    Conjugation of synthetic polymers to peptides offers an efficient way to produce novel supramolecular structures. Herein, we report an attempt to prepare synthetic micellar nanoparticles using amphiphilic peptide-polymer conjugates as molecular building blocks. Spherical nanoparticles were formed upon dissolution of peptides in PBS buffer through the segregation of hydrophobic and hydrophilic segments. Both molecular and nano- structures were thoroughly investigated by a variety of biophysical techniques, including circular dichroism (CD), dynamic light scattering (DLS), size exclusion chromatography (SEC), transmission electron microscopy (TEM) and small angle X-ray scattering (SAXS). The results demonstrate that structural properties of these biohybrid materials depend on both the geometry of the hydrophobic domain and the size of synthetic polymers. Given the diversity of functional peptide sequences, hydrophilic polymers and hydrophobic moieties, these materials would be expected to self-assemble into various types of nanostructures to cover a wide range of biological applications.

  14. Phosphopeptide enrichment by immobilized metal affinity chromatography

    DEFF Research Database (Denmark)

    Thingholm, Tine E.; Larsen, Martin R.


    Immobilized metal affinity chromatography (IMAC) has been the method of choice for phosphopeptide enrichment prior to mass spectrometric analysis for many years and it is still used extensively in many laboratories. Using the affinity of negatively charged phosphate groups towards positively...... charged metal ions such as Fe3+, Ga3+, Al3+, Zr4+, and Ti4+ has made it possible to enrich phosphorylated peptides from peptide samples. However, the selectivity of most of the metal ions is limited, when working with highly complex samples, e.g., whole-cell extracts, resulting in contamination from...

  15. Cooling and exhumation of continents at billion-year time scales (United States)

    Blackburn, T.; Bowring, S. A.; Perron, T.; Mahan, K. H.; Dudas, F. O.


    Hat Block collided at ~1.8 Ga. Rutile U-Pb data from multiple xenoliths, each exhumed from a different depth within the crustal column reveal a range of dates that varies as a function of xenolith residence depth. The shallowest mid- to lower crustal xenoliths (~25 km) cooled first, yielding the youngest dates and yet cooled at rates between 0.1-0.25 °C/Ma over 500 My or more. Deeper xenoliths record cooling at progressively younger times at similar rates and time-scales. From orogony to eruption of xenoliths onto the surface, the lithospheric thermal history constructed using this technique may exceed a billion years. Combining this cooling history with a lithosphere thermal model yields an estimate for the average integrated rate of craton erosion between 0.00-<0.0025 km/Ma across the orogen; a range far lower than the geologically recent to present day rates for continental erosion (<0.005-0.1 km/Ma). This marks the first ever determination of continental exhumation rates on time-scales that approach the age of the continents themselves and has implications for secular cooling of the asthenosphere.

  16. Effects of changes in vegetation on precipitation in the northern Tianshan Mountains evaluated using multiple time scales

    Indian Academy of Sciences (India)

    Qinming Qinming Sun; Tong Liu; Zhiquan Han; Yongping Wu; Bai-Lian Li


    This study used a combination of the wavelet cross-correlation technique and numerical analysis ofvegetative feedback to study the role of climate–vegetation feedback from 1981 to 2009 in the northernTianshan Mountains, Xinjiang Province, China. The study area included the Irtysh River, the Bortalaand Ili River valleys, the northern slopes of the Tianshan Mountains, and the western Junggar Basin.The feedback effects of changes in vegetation on precipitation appeared to vary in these five regionswhen different time scales are used to examine them. The most useful time scale was generally found tobe 4–6 months. Time lag was another characteristic of this process, and the optimal time lag was 3–4months. Nevertheless, optimal time scale and time lag did not differ significantly in these five regions. Inthis way, the correct time scale of the effects of variations in vegetation on precipitation in this cold, aridarea was found. This time scale and time lag can be assessed through wavelet cross-correlation analysis.Then numerical analysis can be used to improve the accuracy of the analysis.

  17. Influence of baryonic physics on the merger time-scale of galaxies in N-body/hydrodynamical simulations

    CERN Document Server

    Jiang, C Y; Lin, W P


    Following our previous work(Jiang et al.(2008)), in which we studied the merger time-scale of galaxies in a high-resolution cosmological hydro/N-body simulation, we investigate the potential influence of uncertainties in the numerical implementation of baryonic physics on the merger time-scale. The simulation used in the previous work suffers from the overcooling problem which causes the central galaxies of large halos too massive. This may result in a shortened merger time-scale compared to that in the real universe. We run a similar simulation, but the stellar mass is significantly reduced to model another extreme case of low stellar mass. Our result shows that the merger time-scale is little affected by the star formation recipes, except for the satellites in nearly radial orbits which show a 22 percent higher time-scale in the lower stellar mass case. Since the radial orbits only account for a small part of the satellites' orbits, the fitting formula in Jiang et al.(2008) is still applicable to a reasonab...

  18. Climatic changes on orbital and sub-orbital time scale recorded by the Guliya ice core in Tibetan Plateau

    Institute of Scientific and Technical Information of China (English)

    姚檀栋; 徐柏青; 蒲健辰


    Based on ice core records in the Tibetan Plateau and Greenland, the features and possible causes of climatic changes on orbital and sub-orbital time scale were discussed. Orbital time scale climatic change recorded in ice core from the Tibetan Plateau is typically ahead of that from polar regions, which indicates that climatic change in the Tibetan Plateau might be earlier than polar regions. The solar radiation change is a major factor that dominates the climatic change on orbital time scale. However, climatic events on sub-orbital time scale occurred later in the Tibetan Plateau than in the Arctic Region, indicating a different mechanism. For example, the Younger Dryas and Heinrich events took place earlier in Greenland ice core record than in Guliya ice core record. It is reasonable to propose the hypothesis that these climatic events were affected possibly by the Laurentide Ice Sheet. Therefore, ice sheet is critically important to climatic change on sub-orbital time scale in some ice ages.

  19. A short-time scale colloidal system reveals early bacterial adhesion dynamics.

    Directory of Open Access Journals (Sweden)

    Christophe Beloin


    Full Text Available The development of bacteria on abiotic surfaces has important public health and sanitary consequences. However, despite several decades of study of bacterial adhesion to inert surfaces, the biophysical mechanisms governing this process remain poorly understood, due, in particular, to the lack of methodologies covering the appropriate time scale. Using micrometric colloidal surface particles and flow cytometry analysis, we developed a rapid multiparametric approach to studying early events in adhesion of the bacterium Escherichia coli. This approach simultaneously describes the kinetics and amplitude of early steps in adhesion, changes in physicochemical surface properties within the first few seconds of adhesion, and the self-association state of attached and free-floating cells. Examination of the role of three well-characterized E. coli surface adhesion factors upon attachment to colloidal surfaces--curli fimbriae, F-conjugative pilus, and Ag43 adhesin--showed clear-cut differences in the very initial phases of surface colonization for cell-bearing surface structures, all known to promote biofilm development. Our multiparametric analysis revealed a correlation in the adhesion phase with cell-to-cell aggregation properties and demonstrated that this phenomenon amplified surface colonization once initial cell-surface attachment was achieved. Monitoring of real-time physico-chemical particle surface properties showed that surface-active molecules of bacterial origin quickly modified surface properties, providing new insight into the intricate relations connecting abiotic surface physicochemical properties and bacterial adhesion. Hence, the biophysical analytical method described here provides a new and relevant approach to quantitatively and kinetically investigating bacterial adhesion and biofilm development.

  20. Possible petrological controls on the location and time scale of slow slip in SW Japan (United States)

    Wallis, S.; Mizukami, T.; Yokoyama, H.; Hiramatsu, Y.; Arai, S.; Kawahara, H.; Nagaya, T.


    To examine the possibility that there was a petrological control on the location and nature of episodic tremor and slip (ETS), we compared the petrological characteristics of wedge mantle material to the results of recent geophysical observations in the Shikoku area, southwest Japan. This study revealed a close relationship between predicted mineral assemblages in the mantle wedge and the characteristics of slow slip behaviour recorded in the Shikoku area: Short-term ETSs take place in the antigorite +olivine stability field and silent long-term slow slip events (SSEs) take place in the antigorite+brucite stability field. The petrology of the mantle wedge may be an important control on the fluid pressure along the subduction interface and influence the time scales of SSEs. The Cretaceous Sanbagawa oceanic subduction complex of SW Japan preserves fragments of the former mantle wedge in contact with subducted slab units. P-T paths and peak P-T conditions show this belt formed as the result of subduction of a young slab under relatively warm conditions. These characteristics make the Sanbagawa belt a good analogue to modern warm subduction zones such as the Philippine Sea subduction zone beneath SW Japan and offer the possibility of directly examining the former plate boundary. Mantle wedge units derived from shallow depths show evidence for widely developed primary brucite and antigorite. In contrast, units derived from greater depths and higher peak temeratures consist dominantly of antigorite and olivine. Observations of the natural serpentinites suggest that the shallow serpentinite with brucite shows higher absorbency of water and provides fluid pathways that can reduce the fluid pore pressure on the subduction boundary.

  1. Nitrogen Cycling in the Black Sea on Glacial-Interglacial Time Scales (United States)

    Quan, T. M.; Wright, J. D.; Falkowski, P. G.


    On geological time scales, the nitrogen cycle consists of three main processes: nitrogen fixation, nitrification, and denitrification/anammox, all of which are affected by the amount of oxygen present in the water column. The balance between nitrogen fixation and denitrification/anammox controls the pool of fixed inorganic nitrogen, and can be traced using nitrogen isotopes. We have previously proposed a conceptual model that relates sedimentary δ15N to deep water oxygen concentrations using a simple, non-linear curve. Provided that the sediment record is well-preserved, the nitrogen isotopic profile at a given location reflects history of the relative contributions of nitrogen fixation to denitrifiation and thus the past redox environment. Our goal was to test this model using deep sea sediments from the Black Sea, which alternates from an anoxic, stratified, marine water body during interglacial periods to a less saline, oxygenated lake during glacial events. We find that the measured δ15N profile in general corresponds to the changes in redox state as determined by trace metal concentrations and micropaleontology. Higher δ15N values occur during suboxic transitional periods, with lower δ15N values appearing under anoxic and oxic conditions, supporting our hypothesis that the nitrogen cycle is regulated by oxygen concentration. We also identify some intervals where the correlation between redox proxies appears to fail. While the lack of ancillary data from the paleo-Black Sea makes interpretation difficult, we believe analysis of these intervals may provide more information about Black Sea paleoenvironmental and paleoredox conditions.

  2. Relationship between the Arctic oscillation and surface air temperature in multi-decadal time-scale (United States)

    Tanaka, Hiroshi L.; Tamura, Mina


    In this study, a simple energy balance model (EBM) was integrated in time, considering a hypothetical long-term variability in ice-albedo feedback mimicking the observed multi-decadal temperature variability. A natural variability was superimposed on a linear warming trend due to the increasing radiative forcing of CO2. The result demonstrates that the superposition of the natural variability and the background linear trend can offset with each other to show the warming hiatus for some period. It is also stressed that the rapid warming during 1970-2000 can be explained by the superposition of the natural variability and the background linear trend at least within the simple model. The key process of the fluctuating planetary albedo in multi-decadal time scale is investigated using the JRA-55 reanalysis data. It is found that the planetary albedo increased for 1958-1970, decreased for 1970-2000, and increased for 2000-2012, as expected by the simple EBM experiments. The multi-decadal variability in the planetary albedo is compared with the time series of the AO mode and Barents Sea mode of surface air temperature. It is shown that the recent AO negative pattern showing warm Arctic and cold mid-latitudes is in good agreement with planetary albedo change indicating negative anomaly in high latitudes and positive anomaly in mid-latitudes. Moreover, the Barents Sea mode with the warm Barents Sea and cold mid-latitudes shows long-term variability similar to planetary albedo change. Although further studies are needed, the natural variabilities of both the AO mode and Barents Sea mode indicate some possible link to the planetary albedo as suggested by the simple EBM to cause the warming hiatus in recent years.

  3. Ventilation of the Black Sea pycnocline on seasonal and interannual time scales

    Directory of Open Access Journals (Sweden)



    Full Text Available The paper is a description of temporal variability of winter cooling conditions and estimation of effective cross-isopycnal mixing rates in the Black Sea. Data averaging versus salinity / sigma-t scale was used to filter effects of local dynamics. It is shown that traces of winter mixing events appear well preserved in the temperature-salinity structure, due to the peculiarities of the Black Sea where temperature often acts as a passive tracer with a smaller contribution to density as compared to salinity. Vertical distribution of the magnitudes of temperature oscillations indicates that the convection events have limited effects in modifying the structure of the middle and lower pycnocline on a seasonal time scale. However, long-term fluctuations are well recognised. The magnitudes of the seasonal and long-term temperature fluctuations are comparable only in the upper pycnocline. Three major cooling events can be distinguished from the record of the pycnocline temperature for the past 75 years. The intensive cooling occurred in the late 1920s - early 1930s, early 1950s and late 1980s - early 1990s. Partial renewal of the water of the cold intermediate layer core took place approximately once in two years. The period when convection causes erosion of the pycnocline lasts for only a week. It is shown that a lateral source of heat and salt exists for the upper pycnocline, where it is the cold intermediate water, and for the lower pycnocline, the layer below S@ 20.5, where this lateral source of salt and heat is maintained by disintegrating Bosphorus plume.

  4. A search for long-time-scale, low-frequency radio transients (United States)

    Murphy, Tara; Kaplan, David L.; Croft, Steve; Lynch, Christene; Callingham, J. R.; Bannister, Keith; Bell, Martin E.; Hurley-Walker, Natasha; Hancock, Paul; Line, Jack; Rowlinson, Antonia; Lenc, Emil; Intema, H. T.; Jagannathan, P.; Ekers, Ronald D.; Tingay, Steven; Yuan, Fang; Wolf, Christian; Onken, Christopher A.; Dwarakanath, K. S.; For, B.-Q.; Gaensler, B. M.; Hindson, L.; Johnston-Hollitt, M.; Kapińska, A. D.; McKinley, B.; Morgan, J.; Offringa, A. R.; Procopio, P.; Staveley-Smith, L.; Wayth, R.; Wu, C.; Zheng, Q.


    We present a search for transient and highly variable sources at low radio frequencies (150-200 MHz) that explores long time-scales of 1-3 yr. We conducted this search by comparing the TIFR GMRT Sky Survey Alternative Data Release 1 (TGSS ADR1) and the GaLactic and Extragalactic All-sky Murchison Widefield Array (GLEAM) survey catalogues. To account for the different completeness thresholds in the individual surveys, we searched for compact GLEAM sources above a flux density limit of 100 mJy that were not present in the TGSS ADR1; and also for compact TGSS ADR1 sources above a flux density limit of 200 mJy that had no counterpart in GLEAM. From a total sample of 234 333 GLEAM sources and 275 612 TGSS ADR1 sources in the overlap region between the two surveys, there were 99 658 GLEAM sources and 38 978 TGSS ADR sources that passed our flux density cut-off and compactness criteria. Analysis of these sources resulted in three candidate transient sources. Further analysis ruled out two candidates as imaging artefacts. We analyse the third candidate and show it is likely to be real, with a flux density of 182 ± 26 mJy at 147.5 MHz. This gives a transient surface density of ρ = (6.2 ± 6) × 10-5 deg-2. We present initial follow-up observations and discuss possible causes for this candidate. The small number of spurious sources from this search demonstrates the high reliability of these two new low-frequency radio catalogues.

  5. The plumbing of the global biological pump: Efficiency control through leaks, pathways, and time scales (United States)

    Pasquier, Benoît; Holzer, Mark


    We systematically quantify the pathways and time scales that set the efficiency, Ebio, of the global biological pump by applying Green-function-based diagnostics to a data-assimilated phosphorus cycle embedded in a jointly assimilated ocean circulation. We consider "bio pipes" that consist of phosphorus paths that connect specified regions of last biological utilization with regions where regenerated phosphate first reemerges into the euphotic zone. The bio pipes that contribute most to Ebio connect the Eastern Equatorial Pacific (EEqP) and Equatorial Atlantic to the Southern Ocean ((21 ± 3)% of Ebio), as well as the Southern Ocean to itself ((15 ± 3)% of Ebio). The bio pipes with the largest phosphorus flow rates connect the EEqP to itself and the subantarctic Southern Ocean to itself. The global mean sequestration time of the biological pump is 130 ± 70 years, while the sequestration time of the bio pipe from anywhere to the Antarctic region of the Southern Ocean is 430 ± 30 years. The distribution of phosphorus flowing within a given bio pipe is quantified by its transit-time partitioned path density. For the largest bio pipes, ˜1/7 of their phosphorus is carried by thermocline paths with transit times less than ˜300-400 years, while ˜4/7 of their phosphorus is carried by abyssal paths with transit times exceeding ˜700 years. The path density reveals that Antarctic Intermediate Water carries about a third of the regenerated phosphate last utilized in the EEqP that is destined for the Southern Ocean euphotic zone. The Southern Ocean is where (62 ± 2)% of the regenerated inventory and (69 ± 1)% of the preformed inventory first reemerge into the euphotic zone.

  6. Determination of paleoseismic activity over a large time-scale: Fault scarp dating with 36Cl (United States)

    Mozafari Amiri, Nasim; Tikhomirov, Dmitry; Sümer, Ökmen; Özkaymak, Çaǧlar; Uzel, Bora; Ivy-Ochs, Susan; Vockenhuber, Christof; Sözbilir, Hasan; Akçar, Naki


    Bedrock fault scarps are the most direct evidence of past earthquakes to reconstruct seismic activity in a large time-scale using cosmogenic 36Cl dating if built in carbonates. For this method, a surface along the fault scarp with a minimum amount of erosion is required to be chosen as an ideal target point. The section of the fault selected for sampling should cover at least two meters of the fault surface from the lower part of the scarp, where intersects with colluvium wedge. Ideally, sampling should be performed on a continuous strip along the direction of the fault slip direction. First, samples of 10 cm high and 15 cm wide are marked on the fault surface. Then, they are collected using cutters, hammer and chisel in a thickness of 3 cm. The main geometrical factors of scarp dip, scarp height, top surface dip and colluvium dip are also measured. Topographic shielding in the sampling spot is important to be estimated as well. Moreover, density of the fault scarp and colluvium are calculated. The physical and chemical preparations are carried in laboratory for AMS and chemical analysis of the samples. A Matlab® code is used for modelling of seismically active periods based on increasing production rate of 36Cl following each rupture, when a buried section of a fault is exposed. Therefore, by measuring the amount of cosmogenic 36Cl versus height, the timing of major ruptures and their offsets are determined. In our study, Manastır, Mugırtepe and Rahmiye faults in Gediz graben, Priene-Sazlı, Kalafat and Yavansu faults in Büyük Menderes graben and Ören fault in Gökava half-graben have been examined in the seismically active region of Western Turkey. Our results reconstruct at least five periods of high seismic activity during the Holocene time, three of which reveal seismic ruptures beyond the historical pre-existing data.

  7. "Tectonic Petrameter," An Alternative Method to Teaching the Geologic Time Scale (United States)

    Posner, E. S.


    I have over a decade of experience as a performance poet and am now a graduate student in the geosciences. I have created a performance poem / play script, "Tectonic Petrameter," as an alternative method of teaching the geologic time scale. "The Archean came next and it was a blast. Tectonic plates were smaller and they moved pretty fast. In an enthusiastic flash of ash, volcanic islands smashed together." The use of rhyme and rhythm presents a different and interdisciplinary approach to teaching Earth history that appeals to a wide range of learning styles and makes science fun, while clearly describing important concepts in geology and events in Earth history. "Now it's time to get down with the Coal Swamp Stomp! Tap your feet to the beat of the formation of peat like a plant plantation soaking up the bright heat." "Tectonic Petrameter" by itself is an illustrated spoken-word poem that leads audiences from all levels of scientific background on an excitingly educational journey through geologic time. I will perform my 10-minute memorized poem and present results from my ongoing study to assess the effectiveness of "Tectonic Petrameter" as a teaching tool in K-12 and introductory undergraduate classroom curricula. I propose that using "Tectonic Petrameter" as a performance piece and theatrical play script in K-12 and introductory undergraduate classrooms, as well as in broader community venues, may be an avenue for breaking down barriers related to teaching about Earth's long and complex history. Digital copies of "Tectonic Petrameter" will be made available to interested parties.

  8. Hydrological connectivity of hillslopes and streams: Characteristic time scales and nonlinearities (United States)

    McGuire, Kevin J.; McDonnell, Jeffrey J.


    Subsurface flow from hillslopes is widely recognized as an important contributor to streamflow generation; however, processes that control how and when hillslopes connect to streams remain unclear. We investigated stream and hillslope runoff dynamics through a wet-up period in watershed 10 of the H. J. Andrews Experimental Forest in the western Cascades of Oregon where the riparian zone has been removed by debris flows. We examined the controls on hillslope-stream connectivity on the basis of observations of hydrometric, stable isotope, and applied tracer responses and computed transit times for multiple runoff components for a series of storms during the wet-up phase of the 2002-2003 winter rainy season. Hillslope discharge was distinctly threshold-like with a near linear response and average quick flow ratio of 0.58 when antecedent rainfall was greater than 20 mm. Hillslope and stream stormflow varied temporally and showed strong hysteretic relationships. Event water mean transit times (8-34 h) and rapid breakthrough from applied hillslope tracer additions demonstrated that subsurface contributing areas extend far upslope during events. Despite rapid hillslope transport processes during events, soil water and runoff mean transit times during nonstorm conditions were greater than the time scale of storm events. Soil water mean transit times ranged between 10 and 25 days. Hillslope seepage and catchment base flow mean transit times were between 1 and 2 years. We describe a conceptual model that captures variable physical flow pathways, their synchronicity, threshold activation, hysteresis, and transit times through changing antecedent wetness conditions that illustrate the different stages of hillslope and stream connectivity.

  9. On the variability of Pacific Ocean tides at seasonal to decadal time scales: Observed vs modelled (United States)

    Devlin, Adam Thomas

    forward in time to the predicted sea level in 2100. Results suggest that stations with large positive combined A-TATs produce total water levels that are greater than those predicted by an increase in MSL alone, increasing the chances of high-water events. Part II examines the mechanisms behind the yearly (TAT) variability in the Western Tropical Pacific Ocean. Significant amplitude TATs are found at more than half of 26 gauges for each of the two strongest tidal constituents, K1 (diurnal) and M2 (semidiurnal). For the lesser constituents analyzed (O1 and S2), significant trends are observed at ten gauges. Part III analyzes the seasonal behavior of tides (STATs) at twenty tide gauges in the Southeast Asian waters, which exhibit variation by 10 -- 30% of mean tidal amplitudes. A barotropic ocean tide model that considers the seasonal effects of MSL, stratification, and geostrophic and Ekman velocity is used to explain the observed seasonal variability in tides due to variations in monsoon-influenced climate forcing, with successful results at about half of all gauges. The observed changes in tides are best explained by the influence of non-tidal velocities (geostrophic and Ekman), though the effect of changing stratification is also an important secondary causative mechanism. From the results of these surveys and investigations, it is concluded that short-term fluctuations in MSL and tidal properties at multiple time scales may be as important in determining the state of future water levels as the long-term trends. Global explanations for the observed tidal behavior have not been found in this study; however, significant regional explanations are found at the yearly time scale in the Solomon Sea, and at the seasonal time scale in Southeast Asia. It is likely that tidal sensitivity to annual and seasonal variations in MSL at other locations also are driven by locally specific processes, rather than factors with basin-wide coherence. (Abstract shortened by ProQuest.).

  10. Affinity purification of copper chelating peptides from chickpea protein hydrolysates. (United States)

    Megías, Cristina; Pedroche, Justo; Yust, Maria M; Girón-Calle, Julio; Alaiz, Manuel; Millan, Francisco; Vioque, Javier


    Chickpea protein hydrolysates obtained with alcalase and flavourzyme were used for purification of copper chelating peptides by affinity chromatography using copper immobilized on solid supports. The chelating activity of purified peptides was indirectly measured by the inhibition of beta-carotene oxidation in the presence of copper. Two protein hydrolysates, obtained after 10 and 100 min of hydrolysis, were the most inhibitory of beta-carotene oxidation. Purified copper chelating peptides from these protein hydrolysates contained 19.7 and 35.1% histidine, respectively, in comparison to 2.7 and 2.6% in the protein hydrolysates. Chelating peptides from hydrolysate obtained after 10 min of hydrolysis were the most antioxidative being 8.3 times more antioxidative than the hydrolysate, while chelating peptides purified from protein hydrolysate obtained after 100 min were 3.1 times more antioxidative than its hydrolysate. However, the histidine content was higher in peptides derived from the 100 min hydrolysate (19.7 against 35.1% in 10 min hydrolysate), indicating that this amino acid is not the only factor involved in the antioxidative activity, and other factors such as peptide size or amino acid sequence are also determinant. This manuscript shows that affinity chromatography is a useful procedure for purification of copper chelating peptides. This method can be extended to other metals of interest in nutrition, such as calcium, iron, or zinc. Purified chelating peptides, in addition to their antioxidative properties, may also be useful in food mineral fortification for increasing the bioavailability of these metals.

  11. The Influence of Time Scale on the Quantitative Study of Soil and Water Conservation Effect of Grassland

    Institute of Scientific and Technical Information of China (English)

    Xiaoxia; WU; Zhujun; GU


    Quantitative analysis of time scale effects is conducive to further understanding of vegetation water and soil conservation mechanism.Based on the observation data of the grass covered and bare soil( control) experimental plots located in Hetian Town,Changting County of Fujian Province from 2007 to 2010,the characteristics of 4 parameters( precipitation,vegetation,RE and SE) were analyzed at precipitation event,month,season,and annual scales,and then the linear regression models were established to describe the relationships between RE( SE)and its influencing factors of precipitation and vegetation. RE( SE) means the ratio of runoff depth( soil loss) of grass covered plot to that of the control plot. Results show that these 4 parameters presented different magnitude and variation on different time scales. RE and SE were relatively stable either within or among different time scales due to their ratios reducing the influence of other factors. The coupling of precipitation and vegetation led to better water conservation effect at lower RE( 0. 7) REs at precipitation event scale as well as at annual scale( R2> 0. 78). For the soil conservation effect,precipitation or / and vegetation was / were the dominated influence factor( s) at precipitation event and annual scales,and the grass LAI could basically describe the positive conservation effect( SE 0. 55),while the maximum 30 min intensity( I30) could describe the negative conservation effect more accurately( SE >1,R2> 0. 79). More uncertainties( R2≈0. 4) exist in the models of both RE and SE at two moderate time scales( month and season). Consequently,factors influencing water and soil conservation effect of grass present different variation and coupling characteristics on different time scales,indicating the importance of time scale at the study on water and soil conservation.

  12. Instrumentation: Ion Chromatography. (United States)

    Fritz, James S.


    Discusses the importance of ion chromatography in separating and measuring anions. The principles of ion exchange are presented, along with some applications of ion chromatography in industry. Ion chromatography systems are described, as well as ion pair and ion exclusion chromatography, column packings, detectors, and programming. (TW)

  13. Delta-Nabla Type Maximum Principles for Second-Order Dynamic Equations on Time Scales and Applications

    Directory of Open Access Journals (Sweden)

    Jiang Zhu


    Full Text Available Some delta-nabla type maximum principles for second-order dynamic equations on time scales are proved. By using these maximum principles, the uniqueness theorems of the solutions, the approximation theorems of the solutions, the existence theorem, and construction techniques of the lower and upper solutions for second-order linear and nonlinear initial value problems and boundary value problems on time scales are proved, the oscillation of second-order mixed delat-nabla differential equations is discussed and, some maximum principles for second order mixed forward and backward difference dynamic system are proved.

  14. Determination of peptide and protein diversity in venom of the spider Selenocosmia jiafu by high performance liquid chromatography and mass spectrometry%采用高效液相色谱-质谱考察家福捕鸟蛛粗毒中多肽和蛋白质的多样性

    Institute of Scientific and Technical Information of China (English)

    胡朝暾; 肖震; 周熙; 陈佳; 陈波; 刘中华


    solution B( acetonitrile with 0. 1% trifluoroacetic acid)over 50 min with a flow rate of 1 mL/min. The isolated venom proteins were treated with in-gel digestion separated by SDS-PAGE and then identified by liquid chromatography-electrospray ionization quadrupole-time of flight mass spectrometry( LC-ESI-QTOF-MS)techniques. The results show that more than 40 fractions eluted were monitored at 215 nm in the RP-HPLC chromatogram of the venom of the spider S. jiafu. Most of the fractions were eluted with retention times of 5-15 min and 25-40 min,corresponding to 5% -15% and 25% -40% acetonitrile,respectively. The venom contains 238 peptides that follow a bimodal distribution,with about 62. 5% of the peptides having a rela-tive molecular mass of 3 000-4 500 and about 33. 2% of the peptides having a relative molecular mass of 1 000-3 000. This distribution model is rather different from those of peptides from oth-er tarantula spider venoms analyzed. To explore the relative molecular mass distribution of the venom proteins,the venom was analyzed by SDS-PAGE using standard protocols. Except for peptides with relative molecular mass lower than 10 000, the SDS-PAGE electrophoresis revealed three more obvious bands around 50,72 and 90 kD respectively. Further MS analysis indicated that there are mainly hemocyanin,potassium ion channel protein,calcium protease and so on. Altogether,this study not only indicated there are many peptides and proteins in the S. jiafu venom,but also provided a basis for further case-by-case investigation of peptide tox-ins from this venom.

  15. Simultaneous Determination of 5 Peptide Antibiotics in Bovine Milk Samples by Liquid Chromatography-Tandem Mass Spectrometry%液相色谱-串联质谱法测定牛奶中5种多肤类抗生素

    Institute of Scientific and Technical Information of China (English)

    刘佳佳; 金芬; 佘永新; 刘洪斌; 史晓梅; 王淼; 王静; 徐思远


    建立了牛奶中杆菌肽、粘杆菌素A、粘杆菌素B、维吉尼霉素和万占霉素5种多肽类抗生素的反相液相色谱-串联质谱(HPLC-MS/MS)检测方法.牛奶样品经甲醇-0.1%甲酸水提取后,用4%三氯乙酸乙睛除蛋白,液-液萃取后,采用0.1%甲酸(A)和0.1%甲酸乙腈(B)作为流动相进行梯度洗脱.质谱(ESI+)采用多离子检测模式(MRM)对两价态或三价态的定性和定量离子进行监测.结果表明,5种多肽类抗生素在25,50和100 μg/kg的添加水平的回收率为75.1%~120.1%;相对标准偏差小于15.7%;方法检出限为0.2~5.6 μg/kg.本方法前处理操作快速简单,重复性好,满足对牛奶中多肤类抗生素的快速、准确的检测要求,适合大量样品的准确定性和定量分析.%A sensitive liquid chromatography tandem mass spectrometric (LC-MS/MS) method has been developed for simultaneous determination of bacitracin, colistin A, colistin B, virginiamycin and vancomycin in bovine milk samples. Milk samples were extracted with a mixture of methanol 0. 1% formic acid and water, and deproteinized with 4% trichloroacetic acid in acetonitrile. The five peptide antibiotics in the extract were separated on a reversed phase using a gradient elution program of 0. 1% formic acid aqueous solution (A) and 0. 1% formic acid in acetonitrile solution (B). Using LC-MS/MS (ESI) with multiple reaction monitoring (MRM), identification of the major components of the five kinds of peptide antibiotics was performed based upon the intensities of mass fragments from the respective doubly or triply charged precursor ions. The recoveries were 75.1%-120. 1% for the five polypeptides at spiked levels of 25, 50 and 100 tg/kg, and the relative standard deviation (RSD) was less than 15.7%. The limit of detection (LOD) for the five peptide antibiotics was 0.2-5.6 μg/kg. The method is suitable for quantitative and qualitative analysis of peptide antibiotics in a sufficient number of

  16. Assessments of Drought Impacts on Vegetation in China with the Optimal Time Scales of the Climatic Drought Index. (United States)

    Li, Zheng; Zhou, Tao; Zhao, Xiang; Huang, Kaicheng; Gao, Shan; Wu, Hao; Luo, Hui


    Drought is expected to increase in frequency and severity due to global warming, and its impacts on vegetation are typically extensively evaluated with climatic drought indices, such as multi-scalar Standardized Precipitation Evapotranspiration Index (SPEI). We analyzed the covariation between the SPEIs of various time scales and the anomalies of the normalized difference vegetation index (NDVI), from which the vegetation type-related optimal time scales were retrieved. The results indicated that the optimal time scales of needle-leaved forest, broadleaf forest and shrubland were between 10 and 12 months, which were considerably longer than the grassland, meadow and cultivated vegetation ones (2 to 4 months). When the optimal vegetation type-related time scales were used, the SPEI could better reflect the vegetation's responses to water conditions, with the correlation coefficients between SPEIs and NDVI anomalies increased by 5.88% to 28.4%. We investigated the spatio-temporal characteristics of drought and quantified the different responses of vegetation growth to drought during the growing season (April-October). The results revealed that the frequency of drought has increased in the 21st century with the drying trend occurring in most of China. These results are useful for ecological assessments and adapting management steps to mitigate the impact of drought on vegetation. They are helpful to employ water resources more efficiently and reduce potential damage to human health caused by water shortages.

  17. An astronomical time scale for the Maastrichtian at the Zumaia and Sopelana sections (Basque country, northern Spain)

    DEFF Research Database (Denmark)

    Batenburg, Sietske J.; Gale, Andy S.; Sprovieri, Mario;


    The rhythmically bedded limestone–marl alternations in the coastal cliffs of Sopelana and Zumaia in the Basque country, northern Spain, permit testing and refining of existing Maastrichtian chronologies (latest Cretaceous). The recently established astronomical time scale for the late Maastrichtian...

  18. Time scale of entropic segregation of flexible polymers in confinement: Implications for chromosome segregation in filamentous bacteria


    Arnold, Axel; Jun, Suckjoon


    We report molecular dynamics simulations of the segregation of two overlapping chains in cylindrical confinement. We find that the entropic repulsion between the chains can be sufficiently strong to cause segregation on a time scale that is short compared to the one for diffusion. This result implies that entropic driving forces are sufficiently strong to cause rapid bacterial chromosome segregation.

  19. Time scale of entropic segregation of flexible polymers in confinement: Implications for chromosome segregation in filamentous bacteria (United States)

    Arnold, Axel; Jun, Suckjoon


    We report molecular dynamics simulations of the segregation of two overlapping chains in cylindrical confinement. We find that the entropic repulsion between chains can be sufficiently strong to cause segregation on a time scale that is short compared to the one for diffusion. This result implies that entropic driving forces are sufficiently strong to cause rapid bacterial chromosome segregation.

  20. Ontology-aided annotation, visualization and generalization of geological time-scale information from online geological map services

    NARCIS (Netherlands)

    Ma, X.; Carranza, E.J.M.; Wu, C.; Meer, F.D. van der


    Geological maps are increasingly published and shared online, whereas tools and services supporting information retrieval and knowledge discovery are underdeveloped. In this study, we developed an ontology of geological time scale by using a RDF (Resource Description Framework) model to represent th

  1. Native American Students' Understanding of Geologic Time Scale: 4th-8th Grade Ojibwe Students' Understanding of Earth's Geologic History (United States)

    Nam, Younkyeong; Karahan, Engin; Roehrig, Gillian


    Geologic time scale is a very important concept for understanding long-term earth system events such as climate change. This study examines forty-three 4th-8th grade Native American--particularly Ojibwe tribe--students' understanding of relative ordering and absolute time of Earth's significant geological and biological events. This study also…

  2. On the Oscillation for Second-Order Half-Linear Neutral Delay Dynamic Equations on Time Scales

    Directory of Open Access Journals (Sweden)

    Quanxin Zhang


    Full Text Available We discuss oscillation criteria for second-order half-linear neutral delay dynamic equations on time scales by using the generalized Riccati transformation and the inequality technique. Under certain conditions, we establish four new oscillation criteria. Our results in this paper are new even for the cases of =ℝ and =ℤ.

  3. Assessments of Drought Impacts on Vegetation in China with the Optimal Time Scales of the Climatic Drought Index

    Directory of Open Access Journals (Sweden)

    Zheng Li


    Full Text Available Drought is expected to increase in frequency and severity due to global warming, and its impacts on vegetation are typically extensively evaluated with climatic drought indices, such as multi-scalar Standardized Precipitation Evapotranspiration Index (SPEI. We analyzed the covariation between the SPEIs of various time scales and the anomalies of the normalized difference vegetation index (NDVI, from which the vegetation type-related optimal time scales were retrieved. The results indicated that the optimal time scales of needle-leaved forest, broadleaf forest and shrubland were between 10 and 12 months, which were considerably longer than the grassland, meadow and cultivated vegetation ones (2 to 4 months. When the optimal vegetation type-related time scales were used, the SPEI could better reflect the vegetation’s responses to water conditions, with the correlation coefficients between SPEIs and NDVI anomalies increased by 5.88% to 28.4%. We investigated the spatio-temporal characteristics of drought and quantified the different responses of vegetation growth to drought during the growing season (April–October. The results revealed that the frequency of drought has increased in the 21st century with the drying trend occurring in most of China. These results are useful for ecological assessments and adapting management steps to mitigate the impact of drought on vegetation. They are helpful to employ water resources more efficiently and reduce potential damage to human health caused by water shortages.

  4. Climate Change and Its Effects on Runoff of Kaidu River,Xinjiang, China: A Multiple Time-scale Analysis

    Institute of Scientific and Technical Information of China (English)

    XU Jianhua; CHEN Yaning; JI Minhe; LU Feng


    This paper applied an integrated method combining grey relation analysis, wavelet analysis and statistical analysis to study climate change and its effects on runoff of the Kaidu River at multi-time scales. Major findings are as follows: 1) Climatic factors were ranked in the order of importance to annual runoff as average annual temperature, average temperature in autumn, average temperature in winter, annual precipitation, precipitation in flood season, av-erage temperature in summer, and average temperature in spring. The average annual temperature and annual precipi-tation were selected as the two representative factors that impact the annual runoff. 2) From the 32-year time scale, the annual runoff and the average annual temperature presented a significantly rising trend, whereas the annual precipita-tion showed little increase over the period of 1957-2002. By changing the time scale from 32-year to 4-year, we ob-served nonlinear trends with increasingly obvious oscillations for annual runoff, average annual temperature, and an-nual precipitation. 3) The changes of the runoff and the regional climate are closely related, indicating that the runoff change is the result of the regional climate changes. With time scales ranging from 32-year, 16-year, 8-year and to 4-year, there are highly significant linear correlations between the annual runoff and the average annual temperature and the annual precipitation.

  5. Biogeochemical Filtering of Solute Signals Explored as a Function of Transport and Reaction Time Scales (United States)

    Zanardo, S.; Basu, N. B.; Rao, P. C.


    complex biogeochemistry of pesticides. We calibrated and validated the model against nitrate and atrazine data collected in the Little Vermillion River basin (~400 km2; Illinois, IN). Comparison with data showed an excellent agreement of the model outputs with the observed hydrographs and chemographs. The model was then used to explore the effect of time constants on biogeochemical signatures. Four time constants were used to characterize the coupling of hydrologic and biogeochemical processes considered in the model: 1) mean rainfall frequency; 2) mean residence time; 3) mass-transfer rate constant; 4) degradation rate constant. The effect of the four temporal constants on the hillslope biogeochemical response was explored through a sesnsitivity analyses. We hypothesize that a single dimensionless number, a combination of all four temporal constants, could explain the observed patterns. Because this dimensionless number embeds all of the relevant time scales, relative significance of the hydrologic and biogeochemical processes can be examined.

  6. The Rhine delta. A record of sediment trapping over time scales from millennia to decades

    Energy Technology Data Exchange (ETDEWEB)

    Middelkoop, Hans; Perk, Marcel van der [Utrecht Univ. (Netherlands). Dept. of Physical Geography; Erkens, Gilles [Utrecht Univ. (Netherlands). Dept. of Physical Geography; Deltares, Utrecht (Netherlands)


    At the land-ocean interface, large river deltas are major sinks of sediments and associated matter. Over the past decennia, many studies have been conducted on the palaeogeographic development of the Rhine delta and overbank deposition on the Rhine floodplains. This paper aims to synthesise these research results with special focus on the amounts and changes of overbank fines trapped in the Rhine delta and their controls at different time scales in the past, present and future. Sediment trapping in the Rhine delta throughout the Holocene was quantified using a detailed database of the Holocene delta architecture. Additional historic data allowed the reconstruction of the development of the river's floodplain during the period of direct human modification of the river. Using heavy metals as tracers, overbank deposition rates over the past century were determined. Measurements of overbank deposition and channel bed sediment transport in recent years, together with modelling studies of sediment transport and deposition have provided detailed insight in the present-day sediment deposition on the floodplains, as well as their controls. Estimated annual suspended sediment deposition rates were about 1.4 x 10{sup 9} kg year{sup -1} between 6,000 and 3,000 years BP and increased to about 2.1 x 10{sup 9} kg year{sup -1} between 3,000 and 1,000 years BP. After the rivers were embanked by artificial levees between 1100 and 1300 AD, the amount of sediment trapped in the floodplains reduced to about 1.16 x 10{sup 9} kg year{sup -1}. However, when accounting for re-entrainment of previously deposited sediment, the actual sediment trapping of the embanked floodplains was about 1.86 x 10{sup 9} kg year{sup -1}. Downstream of the lower Waal branch an inland delta developed that trapped another 0.4 x 10{sup 9} kg year{sup -1} of overbank fines. Since the width of channel was artificially reduced and the banks were fixed by a regular array of groynes around 1850, the average

  7. High-performance liquid chromatography of biopolymers

    Energy Technology Data Exchange (ETDEWEB)

    Regnier, F.E.


    The ability to separate biological macromolecules with good resolution on liquid chromatographic columns has depended on the development of suitable packing materials. In size exclusion chromatography, molecules are separated by size on the basis of differential permeation of the packing. Ion exchange, hydrophobic interaction (or reversed-phase), and affinity chromatography are all surface-mediated separation methods, although they depend on different retention mechanisms. High-performance liquid chromatographic columns designed for biopolymers offer major advantages over conventional columns in both speed and resolving power. The exponential growth of literature on the high-performance separation of peptides and proteins in particular indicates that the techniques will become the dominant form of column liquid chromatography. 92 refs., 4 figs.

  8. Analysis of illegal peptide biopharmaceuticals frequently encountered by controlling agencies. (United States)

    Vanhee, Celine; Janvier, Steven; Desmedt, Bart; Moens, Goedele; Deconinck, Eric; De Beer, Jacques O; Courselle, Patricia


    Recent advances in genomics, recombinant expression technologies and peptide synthesis have led to an increased development of protein and peptide therapeutics. Unfortunately this goes hand in hand with a growing market of counterfeit and illegal biopharmaceuticals, including substances that are still under pre-clinical and clinical development. These counterfeit and illegal protein and peptide substances could imply severe health threats as has been demonstrated by numerous case reports. The Belgian Federal Agency for Medicines and Health Products (FAMHP) and customs are striving, together with their global counterparts, to curtail the trafficking and distributions of these substances. At their request, suspected protein and peptide preparations are analysed in our Official Medicines Control Laboratory (OMCL). It stands to reason that a general screening method would be beneficiary in the battle against counterfeit and illegal peptide drugs. In this paper we present such general screening method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the identification of counterfeit and illegal injectable peptide preparations, extended with a subsequent quantification method using ultra-high performance liquid chromatography with diode array detection (UHPLC-DAD). The screening method, taking only 30 min, is able to selectively detect 25 different peptides and incorporates the proposed minimum of five identification points (IP) as has been recommended for sports drug testing applications. The group of peptides represent substances which have already been detected in illegal and counterfeit products seized by different European countries as well as some biopharmaceutical peptides which have not been confiscated yet by the controlling agencies, but are already being used according to the many internet users forums. Additionally, we also show that when applying the same LC gradient, it is also possible to quantify these peptides without the need for

  9. Identification of oxidised proteins in the matrix of rice leaf mitochondria by immunoprecipitation and two-dimensional liquid chromatography-tandem mass spectrometry

    DEFF Research Database (Denmark)

    Kristensen, B.K.; Askerlund, P.; Bykova, N.V.


    of peptides was analysed by nano-HPLC coupled online to an ESI-Quad-TOF mass spectrometer. The peptides were separated by stepwise ion exchange chromatography followed by reverse phase chromatography (2D-LC), and analysed by MS/MS. Proteins were identified by un-interpreted fragment ion database searches...

  10. Sulphonic acid strong cation-exchange restricted access columns in sample cleanup for profiling of endogenous peptides in multidimensional liquid chromatography. Structure and function of strong cation-exchange restricted access materials. (United States)

    Machtejevas, E; Denoyel, R; Meneses, J M; Kudirkaite, V; Grimes, B A; Lubda, D; Unger, K K


    In this work, the pore structural parameters and size exclusion properties of LiChrospher strong cation-exchange and reverse phase restricted access materials (RAM) are analysed. The molecular weight size exclusion limit for polystyrenes was found to be about 17.7 kDa, while for standard proteins, the molecular weight size exclusion limit was higher, at approximately 25 kDa. The average pore diameter on a volume basis calculated from the pore network model changes from 8.5 nm (native LiChrospher) to 8.6 nm (diol derivative) to 8.2 nm (sulphonic acid derivative) to 6.9 nm (n-octadecyl derivative). Additional characterisations were performed on restricted access materials with nitrogen sorption at 77 K, water adsorption at 25 degrees C, intrusion-extrusion of water (in order to evaluate the hydrophobic properties of the pores of the hydrophobic RAM), and zeta potential measurements by microelectrophoresis. For peptide analysis out of the biofluids, the strong cation-exchange functionality seems to be particularly suitable mainly because of the high loadability of the strong cation-exchange restricted access material (SCX-RAM) and the fact that one can work under non-denaturing conditions to perform effective chromatographic separations. For bacitracin, the dynamic capacity of the SCX-RAM columns does not reach its maximum value in the analysed range. For lysozyme, the dynamic capacity reaches a value of 0.08 mg/ml of column volume before column is overloaded. Additionally, the proper column operating conditions that lead to the total effective working time of the RAM column to be equal to approximately 500 injections (depending on the type of sample), is comprehensively described. The SCX-RAM column was used in the same system analysing urine samples for the period of 1 month (approximately 150 injections) with run-to-run reproducibility below 5% RSD and below 10% RSD for the relative fractions.

  11. Human peptide transporters

    DEFF Research Database (Denmark)

    Nielsen, Carsten Uhd; Brodin, Birger; Jørgensen, Flemming Steen;


    Peptide transporters are epithelial solute carriers. Their functional role has been characterised in the small intestine and proximal tubules, where they are involved in absorption of dietary peptides and peptide reabsorption, respectively. Currently, two peptide transporters, PepT1 and PepT2...

  12. Modelling the water balance of a precise weighable lysimeter for short time scales (United States)

    Fank, Johann; Klammler, Gernot; Rock, Gerhard


    Precise knowledge of the water fluxes between the atmosphere and the soil-plant system and the percolation to the groundwater system is of great importance for understanding and modeling water, solute and energy transfer in the atmosphere-plant-soil-groundwater system. Weighable lysimeters yield the most precise and realistic measures for the change of stored water volume (ΔS), Precipitation (P) which can be rain, irrigation, snow and dewfall and evapotranspiration (ET) as the sum of soil evaporation, evaporation of intercepted water and transpiration. They avoid systematic errors of standard gauges and class-A pans. Lysimeters with controlled suction at the lower boundary allow estimation of capillary rise (C) and leachate (L) on short time scales. Precise weighable large scale (surface >= 1 m2) monolithic lysimeters avoiding oasis effects allow to solve the water balance equation (P - ET - L + C ± ΔS = 0) for a 3D-section of a natural atmosphere-plant-soil-system for a certain time period. Precision and accuracy of the lysimeter measurements depend not only on the precision of the weighing device but also on external conditions, which cannot be controlled or turned off. To separate the noise in measured data sets from signals the adaptive window and adaptive threshold (AWAT) filter (Peters et al., 2014) is used. The data set for the years 2010 and 2011 from the HYDRO-lysimeter (surface = 1 m2, depth = 1 m) in Wagna, Austria (Klammler and Fank, 2014) with a resolution of 0,01 mm for the lysimeter scale and of 0,001 mm for the leachate tank scale is used to evaluate the water balance. The mass of the lysimeter and the mass of the leachate tank is measured every two seconds. The measurements are stored as one minute arithmetic means. Based on calculations in a calibration period from January to May 2010 with different widths of moving window the wmax - Parameter for the AWAT filter was set to 41 minutes. A time series for the system mass ('upper boundary') of the

  13. Protein identification using nano liquid chromatography-tandem mass spectrometry. (United States)

    Negroni, Luc


    Tandem mass spectrometry is an efficient technique for the identification of peptides on the basis of their fragmentation pattern (MS/MS scan). It can generate individual spectra for each peptide, thereby creating a powerful tool for protein identification on the basis of peptide characterization. This important advance in automatic data acquisition has allowed an efficient association between liquid chromatography and tandem mass spectrometry, and the use of nanocolumns and nanoelectrospray ionization has dramatically increased the efficiency of this method. Now large sets of peptides can be identified at a femtomole level. At the end of the process, batch processing of the MS/MS spectra produces peptide lists that identify purified proteins or protein mixtures with high confidence.

  14. Development of SI-traceable C-peptide certified reference material NMIJ CRM 6901-a using isotope-dilution mass spectrometry-based amino acid analyses. (United States)

    Kinumi, Tomoya; Goto, Mari; Eyama, Sakae; Kato, Megumi; Kasama, Takeshi; Takatsu, Akiko


    A certified reference material (CRM) is a higher-order calibration material used to enable a traceable analysis. This paper describes the development of a C-peptide CRM (NMIJ CRM 6901-a) by the National Metrology Institute of Japan using two independent methods for amino acid analysis based on isotope-dilution mass spectrometry. C-peptide is a 31-mer peptide that is utilized for the evaluation of β-cell function in the pancreas in clinical testing. This CRM is a lyophilized synthetic peptide having the human C-peptide sequence, and contains deamidated and pyroglutamylated forms of C-peptide. By adding water (1.00 ± 0.01) g into the vial containing the CRM, the C-peptide solution in 10 mM phosphate buffer saline (pH 6.6) is reconstituted. We assigned two certified values that represent the concentrations of total C-peptide (mixture of C-peptide, deamidated C-peptide, and pyroglutamylated C-peptide) and C-peptide. The certified concentration of total C-peptide was determined by two amino acid analyses using pre-column derivatization liquid chromatography-mass spectrometry and hydrophilic chromatography-mass spectrometry following acid hydrolysis. The certified concentration of C-peptide was determined by multiplying the concentration of total C-peptide by the ratio of the relative area of C-peptide to that of the total C-peptide measured by liquid chromatography. The certified value of C-peptide (80.7 ± 5.0) mg/L represents the concentration of the specific entity of C-peptide; on the other hand, the certified value of total C-peptide, (81.7 ± 5.1) mg/L can be used for analyses that does not differentiate deamidated and pyroglutamylated C-peptide from C-peptide itself, such as amino acid analyses and immunochemical assays.

  15. Pomegranin, an antifungal peptide from pomegranate peels. (United States)

    Guo, Guang; Wang, He Xiang; Ng, Tzi Bun


    A new antifungal peptide designated as pomegranin, with an N-terminal sequence resembling that of rice disease resistance NB-S-LRR-like protein, was isolated from fresh pomegranate peels by ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, and gel filtration by fast protein liquid chromatography on Superdex 75. Pomegranin was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel. It exhibited a molecular mass of 11 kDa in both gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It inhibited mycelial growth in the fungi Botrytis cinerea and Fusarium oxysporum with an IC(50) of 2 microM and 6.1 microM, respectively. It was devoid of hemagglutinating, ribonuclease, deoxyribonuclease and protease inhibitory activities.

  16. Basic Principles of Chromatography (United States)

    Ismail, Baraem; Nielsen, S. Suzanne

    Chromatography has a great impact on all areas of analysis and, therefore, on the progress of science in general. Chromatography differs from other methods of separation in that a wide variety of materials, equipment, and techniques can be used. [Readers are referred to references (1-19) for general and specific information on chromatography.]. This chapter will focus on the principles of chromatography, mainly liquid chromatography (LC). Detailed principles and applications of gas chromatography (GC) will be discussed in Chap. 29. In view of its widespread use and applications, high-performance liquid chromatography (HPLC) will be discussed in a separate chapter (Chap. 28). The general principles of extraction are first described as a basis for understanding chromatography.

  17. Application of mimotope peptides of fumonisin b1 in Peptide ELISA. (United States)

    Liu, Xing; Xu, Yang; He, Qing-hua; He, Zhen-yun; Xiong, Zheng-ping


    Anti-fumonisin B(1) (FB(1)) McAb 1D11 was used as the target for biopanning from a phage random loop-constrained heptapeptide library. After three cycles of panning, seven phages with three mimotope peptides were selected to mimic the binding of FB(1) to 1D11. After the identification of phage ELISA, the phage clone that showed the best linear range of detection was chosen for further research. One peptide with the inserted peptide sequence of the phage was synthetized, named CT-452. An indirect competitive ELISA (peptide ELISA) for detecting FB(1) was established using the CT-452-bovine serum albumin conjugate as coating antigen. The linear range of the inhibition curve was 1.77-20.73 ng/mL. The half inhibitory concentration (IC50) was 6.06 ng/mL, and the limit of detection was 1.18 ng/mL. This method was compared with conventional indirect ELISA (commercial ELISA kit) and high-performance liquid chromatography (HPLC), and the results showed the reliability of the peptide ELISA for the determination of FB(1) in cereal samples. The relationship between the CT-452 and FB(1) standard concentrations in peptide ELISA was evaluated. The results indicated that synthetic peptide CT-452 can replace the FB(1) standard to establish an immunoassay free of FB(1).

  18. Computing the transport time scales of a stratified lake on the basis of Tonolli’s model

    Directory of Open Access Journals (Sweden)

    Marco Pilotti


    Full Text Available This paper deals with a simple model to evaluate the transport time scales in thermally stratified lakes that do not necessarily completely mix on a regular annual basis. The model is based on the formalization of an idea originally proposed in Italian by Tonolli in 1964, who presented a mass balance of the water initially stored within a lake, taking into account the known seasonal evolution of its thermal structure. The numerical solution of this mass balance provides an approximation to the water age distribution for the conceptualised lake, from which an upper bound to the typical time scales widely used in limnology can be obtained. After discussing the original test case considered by Tonolli, we apply the model to Lake Iseo, a deep lake located in the North of Italy, presenting the results obtained on the basis of a 30 year series of data.

  19. Reference evapotranspiration models using different time scales in the Jaboticabal region of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Natália Buzinaro Caporusso


    Full Text Available The aim of this paper is to compare 18 reference evapotranspiration models to the standard Penman-Monteith model in the Jaboticabal, São Paulo, region for the following time scales: daily, 5-day, 15-day and seasonal. A total of 5 years of daily meteorological data was used for the following analyses: accuracy (mean absolute percentage error, Mape, precision (R2 and tendency (bias (systematic error, SE. The results were also compared at the 95% probability level with Tukey’s test. The Priestley-Taylor (1972 method was the most accurate for all time scales, the Tanner-Pelton (1960 method was the most accurate in the winter, and the Thornthwaite (1948 method was the most accurate of the methods that only used temperature data in the equations.

  20. Interval Oscillation Criteria for Forced Second-Order Nonlinear Delay Dynamic Equations with Damping and Oscillatory Potential on Time Scales

    Directory of Open Access Journals (Sweden)

    Hassan A. Agwa


    Full Text Available We are concerned with the interval oscillation of general type of forced second-order nonlinear dynamic equation with oscillatory potential of the form rtg1xt,xΔtΔ+p(tg2(x(t,xΔ(txΔ(t+q(tf(x(τ(t=e(t, on a time scale T. We will use a unified approach on time scales and employ the Riccati technique to establish some oscillation criteria for this type of equations. Our results are more general and extend the oscillation criteria of Erbe et al. (2010. Also our results unify the oscillation of the forced second-order nonlinear delay differential equation and the forced second-order nonlinear delay difference equation. Finally, we give some examples to illustrate our results.

  1. Efficient purification of unique antibodies using peptide affinity-matrix columns

    DEFF Research Database (Denmark)

    Jensen, Liselotte Brix; Riise, Erik; Nielsen, Leif Kofoed;


    Phage display technology was used to identify peptide ligands with unique specificity for a monoclonal model antibody, MK16, that recognises the human multiple sclerosis associated MHC class II molecule DR2 in complex with a myelin basic protein (MBP)-derived peptide corresponding to residue 85......-99. Several peptide epitopes were identified and all of them recognised specifically MK16. One peptide, ER6.1, was selected and linked to beaded agarose and demonstrated excellent performance as a peptide affinity chromatography matrix. This epitope matrix was efficient in the purification of MK16 Fab...

  2. Linear regulator design for stochastic systems by a multiple time scales method. [with application to F-8 aircraft longitudinal control (United States)

    Teneketzis, D.; Sandell, N. R., Jr.


    This paper develops a hierarchically-structured, suboptimal controller for a linear stochastic system composed of fast and slow subsystems. It is proved that the controller is optimal in the limit as the separation of time scales of the subsystems becomes infinite. The methodology is illustrated by design of a controller to suppress the phugoid and short period modes of the longitudinal dynamics of the F-8 aircraft.

  3. Noether Theory for Hamiltonian System on Time Scales%时间尺度上Hamilton系统的Noether理论

    Institute of Scientific and Technical Information of China (English)



    提出并研究时间尺度上Hamilton系统的Noether对称性与守恒量问题.建立了时间尺度上Hamilton原理,导出了相应的Hamilton正则方程.基于时间尺度上Hamilton作用量在群的无限小变换下的不变性,建立了时间尺度上Hamilton系统的Noether定理.定理的证明分成两步:第一步,在时间不变的无限小变换群下给出证明;第二步,利用时间重新参数化技术得到了一般无限小变换群下的定理.给出了经典和离散两种情况下Hamilton系统的Noether守恒量.文末举例说明结果的应用.%The Noether symmetry and the conserved quantity for a Hamiltonian system on time scales are proposed and studied in this paper.The Hamilton principle on time scales is established,and corresponding Hamilton canonical equations are deduced.Based upon the invariance of the Hamilton action on time scales under the infinitesimal transformations of a group,the Noether theorem for the Hamiltonian system on time scales is established.The proof of the theorem is composed of two steps.First,we prove the Noether theorem under the infinitesimal transformations of a special one-parameter group without varying the time.Second using the technique of time-re-parameterization,we obtain the Noether theorem in its general form.The Noether-type conserved quantities for Hamiltonian system in both the classical and the discrete cases are given.At the end of the paper,two examples are given to illustrate the application of the theorem.

  4. Release of a single neurotransmitter from an identified interneuron coherently affects motor output on multiple time scales


    Dacks, Andrew M.; Weiss, Klaudiusz R.


    Neurotransmitters can have diverse effects that occur over multiple time scales often making the consequences of neurotransmission difficult to predict. To explore the consequences of this diversity, we used the buccal ganglion of Aplysia to examine the effects of GABA release by a single interneuron, B40, on the intrinsic properties and motor output of the radula closure neuron B8. B40 induces a picrotoxin-sensitive fast IPSP lasting milliseconds in B8 and a slow EPSP lasting seconds. We fou...

  5. The Contribution of Wide Range of Space and Time Scales to the Northward Flux of Westerly Momentum (United States)

    Straus, D. M.


    The contribution of a wide range of time scales to the long time average of atmospheric variances and covariances, and in particular on the contribution of the annual cycle is discussed. The interannual variability of seasonally averaged fluxes is analyzed in the same context. For definiteness, numerical results will be shown for the eddy momentum flux at 200 mb as obtained from seven years of NMC analyses (March 8, 1970 - March 10, 1977). The role of the seasonal cycle is considered.

  6. Quantification of Hydrological Responses Due to Climate Change and Human Activities over Various Time Scales in South Korea

    Directory of Open Access Journals (Sweden)

    Sangho Lee


    Full Text Available Hydrological responses are being impacted by both climate change and human activities. In particular, climate change and regional human activities have accelerated significantly during the last three decades in South Korea. The variation in runoff due to the two types of factors should be quantitatively investigated to aid effective water resources’ planning and management. In water resources’ planning, analysis using various time scales is useful where rainfall is unevenly distributed. However, few studies analyzed the impacts of these two factors over different time scales. In this study, hydrologic model-based approach and hydrologic sensitivity were used to separate the relative impacts of these two factors at monthly, seasonal and annual time scales in the Soyang Dam upper basin and the Seom River basin in South Korea. After trend analysis using the Mann–Kendall nonparametric test to identify the causes of gradual change, three techniques, such as the double mass curve method, Pettitt’s test and the BCP (Bayesian change point analysis, were used to detect change points caused by abrupt changes in the collected observed runoff. Soil and Water Assessment Tool (SWAT models calibrated from the natural periods were used to calculate the impacts of human activities. Additionally, six Budyko-based methods were used to verify the results obtained from the hydrological-based approach. The results show that impacts of climate change have been stronger than those of human activities in the Soyang Dam upper basin, while the impacts of human activities have been stronger than those of climate change in the Seom River basin. Additionally, the quantitative characteristics of relative impacts due to these two factors were identified at the monthly, seasonal and annual time scales. Finally, we suggest that the procedure used in this study can be used as a reference for regional water resources’ planning and management.

  7. Systematic study of the effects of mass and time scaling techniques applied in numerical rock mechanics simulations (United States)

    Heinze, Thomas; Jansen, Gunnar; Galvan, Boris; Miller, Stephen A.


    Numerical modeling is a well established tool in rock mechanics studies investigating a wide range of problems. Implicit methods for solving linear equations have the advantage of being unconditionally stable, while explicit methods, although limited by the time step, are often used because of their limited memory demand, their scalability in parallel computing, and simple implementation of complex boundary conditions. In numerical modeling of explicit elastoplastic dynamics where the time step is limited by the material density, mass scaling techniques can be used to overcome this limit and significantly reduce computation time. While often used, the effect of mass and time scaling and how it may influence the numerical results is rarely-mentioned in publications, and choosing the right scaling technique is typically performed by trial and error. To our knowledge, no systematic studies have addressed how mass scaling might affect the numerical results. In this paper, we present results from an extensive and systematic study of the influence of mass and time scaling on the behavior of a variety of rock-mechanical models. We employ a finite difference scheme to model uniaxial and biaxial compression experiments using different mass and time scaling factors, and with physical models of increasing complexity up to a cohesion-weakening frictional-strengthening model (CWFS). We also introduce a normalized energy ratio to assist analyzing mass scaling effects. We find the tested models to be less sensitive to time scaling than to mass scaling, so mass scaling has higher potential for decreasing computational costs. However, we also demonstrate that mass scaling may lead to quantitatively wrong results, so care must be taken in interpreting stress values when mass scaling is used in complicated rock mechanics simulations. Mass scaling significantly influences the stress-strain response of numerical rocks because mass scaling acts as an artificial hardening agent on rock

  8. Perspective: On the relevance of slower-than-femtosecond time scales in chemical structural-dynamics studies

    Directory of Open Access Journals (Sweden)

    Philip Coppens


    Full Text Available A number of examples illustrate structural-dynamics studies of picosecond and slower photo-induced processes. They include molecular rearrangements and excitations. The information that can be obtained from such studies is discussed. The results are complementary to the information obtained from femtosecond studies. The point is made that all pertinent time scales should be covered to obtain comprehensive insight in dynamic processes of chemical and biological importance.

  9. Estimation of Dynamic VaR in Chinese Stock Markets Based on Time Scale and Extreme Value Theory

    Institute of Scientific and Technical Information of China (English)


    The accuracy and time scale invariance of value-at-risk (VaR) measurement methods for different stock indices and at different confidence levels are tested. Extreme value theory (EVT) is applied to model the extreme tail of standardized residual series of daily/weekly indices losses, and parametric and nonparametric methods are used to estimate parameters of the general Pareto distribution (GPD), and dynamic VaR for indices of three stock markets in China. The accuracy and time scale invariance of risk measurement methods through back-testing approach are also examined. Results show that not all the indices accept time scale invariance; there are some differences in accuracy between different indices at various confidence levels. The most powerful dynamic VaR estimation methods are EVT-GJR-Hill at 97.5% level for weekly loss to Shanghai stock market, and EVT-GARCH-MLE (Hill) at 99.0% level for weekly loss to Taiwan and Hong Kong stock markets, respectively.

  10. Multiple time scale evaluation of the energy balance during the maize growing season, and a new reason for energy imbalance

    Institute of Scientific and Technical Information of China (English)

    GUO JianXia; BIAN LinGen; DAI YongJiu


    Energy imbalance is a common problem associated with the measurement of surface energy using the eddy covariance method. In the evaluation of the energy balance, people usually pay more attention to the statistical result that the effective energy (the sum of sensible and latent heat) is systematically lower than the available energy (the difference of net radiation and ground heat flux). However, little attention has been paid to the existence of the reversed situation when the effective energy is larger than the available energy or their contribution to the overall energy closure rate. In this paper, based on the analysis of the energy balance on multiple time scales across the maize growth season, we con clude that the non-synchronization of energy components is the main reason for the existence of the reversed case. By shifting the phase of the effective energy components half an hour ahead, the rates of energy closure over all time scales are improved and dramatically reduce the number of the half-hourly samples when the energy ratio exceeds 1 or is below 0.5. According to the characteristics of the energy distribution and transformation over multiple time scales, latent heat is always the main type of energy cost, and the residual of the energy balance increases with the growth of the maize plant surpassing the sensible heat for seventy days. It is suggested that the heat storage and photosynthetic energy play an important role in the energy balance during the growing period of maize.

  11. Analysis of proteins and peptides by electromigration methods in microchips. (United States)

    Štěpánová, Sille; Kašička, Václav


    This review presents the developments and applications of microchip electromigration methods in the separation and analysis of peptides and proteins in the period 2011-mid-2016. The developments in sample preparation and preconcentration, microchannel material, and surface treatment are described. Separations by various microchip electromigration methods (zone electrophoresis in free and sieving media, affinity electrophoresis, isotachophoresis, isoelectric focusing, electrokinetic chromatography, and electrochromatography) are demonstrated. Advances in detection methods are reported and novel applications in the areas of proteomics and peptidomics, quality control of peptide and protein pharmaceuticals, analysis of proteins and peptides in biomatrices, and determination of physicochemical parameters are shown.

  12. Electron capture dissociation of singly and multiply phosphorylated peptides

    DEFF Research Database (Denmark)

    Stensballe, A; Jensen, Ole Nørregaard; Olsen, J V


    Analysis of phosphotyrosine and phosphoserine containing peptides by nano-electrospray Fourier transform ion cyclotron resonance (FTICR) mass spectrometry established electron capture dissociation (ECD) as a viable method for phosphopeptide sequencing. In general, ECD spectra of synthetic...... and native phosphopeptides appeared less complex than conventional collision activated dissociation (CAD) mass spectra of these species. ECD of multiply protonated phosphopeptide ions generated mainly c- and z(.)-type peptide fragment ion series. No loss of water, phosphate groups or phosphoric acid from......(III)-affinity chromatography combined with nano-electrospray FTMS/ECD facilitated phosphopeptide analysis and amino acid sequencing from crude proteolytic peptide mixtures....

  13. Automated 2D peptide separation on a 1D nano-LC-MS system

    DEFF Research Database (Denmark)

    Taylor, Paul; Nielsen, Peter A; Trelle, Morten Beck;


    the on-line separation of highly complex peptide mixtures directly coupled with mass spectrometry-based identification. Here, we present a variation of the traditional MudPIT protocol, combining highly sensitive chromatography using a nanoflow liquid chromatography system (nano-LC) with a two...

  14. Truncated glucagon-like peptide I, an insulin-releasing hormone from the distal gut

    DEFF Research Database (Denmark)

    Holst, J J; Orskov, C; Nielsen, O V;


    By hydrophobic gel permeation and high pressure liquid chromatography we isolated from pig intestinal mucosa a peptide which corresponds to proglucagon 78-107 as suggested by chromatography and determination of its N-terminal sequence. Natural and synthetic proglucagon 78-107 dose dependently...

  15. SIMAC - A phosphoproteomic strategy for the rapid separation of mono-phosphorylated from multiply phosphorylated peptides

    DEFF Research Database (Denmark)

    Thingholm, Tine E; Jensen, Ole N; Robinson, Phillip J


    spectrometric analysis, such as immobilized metal affinity chromatography or titanium dioxide the coverage of the phosphoproteome of a given sample is limited. Here we report a simple and rapid strategy - SIMAC - for sequential separation of mono-phosphorylated peptides and multiply phosphorylated peptides from...... and an optimized titanium dioxide chromatographic method. More than double the total number of identified phosphorylation sites was obtained with SIMAC, primarily from a three-fold increase in recovery of multiply phosphorylated peptides....

  16. Toxin Inhibition - Deconvolution Strategies and Assay Screening of Combinatorial Peptide Libraries (United States)


    to micellar electrokinetic chromatography (MEKC) [34, 35, 36]. At 50 mM concentration SDS forms micelles; the negatively charged polar sulfate head...synthetic derivatives of peptide hormones by capillary zone electrophoresis and micellar electrokinetic chromatography with ultraviolet-absorption and...BoNT MI Mean inhibition MEKC Micellar electrokinetic chromatography SNAP-25 Synapotsomal associated Protein 25 kDa S, P1, P2 Substrate and product I

  17. Molecular dynamics simulations of helical antimicrobial peptides in SDS micelles: what do point mutations achieve?

    DEFF Research Database (Denmark)

    Khandelia, Himanshu; Kaznessis, Yiannis N


    We report long time scale simulations of the 18-residue helical antimicrobial peptide ovispirin-1 and its analogs novispirin-G10 and novispirin-T7 in SDS micelles. The SDS micelle serves as an economical and effective model for a cellular membrane. Ovispirin, which is initially placed along a mic...

  18. Purification of a Novel Antibacterial Short Peptide in Earthworm Eisenia foetida

    Institute of Scientific and Technical Information of China (English)

    Yan-Qin LIU; Zhen-Jun SUN; Chong WANG; Shi-Jie LI; Yu-Zhi LIU


    A novel antimicrobial short peptide was purified from earthworm (Eisenia foetida) by a five-step protocol including ammonium sulfate precipitation, ultrafiltration, DE-52 ion exchange chromatography, Sephadex G-10 column chromatography, and C-18 reversed-phase HPLC techniques.The purified peptide was applied to the MALDI-TOP MS to determine the molecular mass and was also subjected to TOF MS-MS analysis to determine the amino acid sequence. As a result, a novel antibacterial peptide, named OEP3121, was obtained, with the molecular mass of 510.8 Da and the sequence being "ACSAG".

  19. PeptideAtlas (United States)

    U.S. Department of Health & Human Services — PeptideAtlas is a multi-organism, publicly accessible compendium of peptides identified in a large set of tandem mass spectrometry proteomics experiments. Mass...

  20. Release of a single neurotransmitter from an identified interneuron coherently affects motor output on multiple time scales. (United States)

    Dacks, Andrew M; Weiss, Klaudiusz R


    Neurotransmitters can have diverse effects that occur over multiple time scales often making the consequences of neurotransmission difficult to predict. To explore the consequences of this diversity, we used the buccal ganglion of Aplysia to examine the effects of GABA release by a single interneuron, B40, on the intrinsic properties and motor output of the radula closure neuron B8. B40 induces a picrotoxin-sensitive fast IPSP lasting milliseconds in B8 and a slow EPSP lasting seconds. We found that the excitatory effects of this slow EPSP are also mediated by GABA. Together, these two GABAergic actions structure B8 firing in a pattern characteristic of ingestive programs. Furthermore, we found that repeated B40 stimulation induces a persistent increase in B8 excitability that was occluded in the presence of the GABA B receptor agonist baclofen, suggesting that GABA affects B8 excitability over multiple time scales. The phasing of B8 activity during the feeding motor programs determines the nature of the behavior elicited during that motor program. The persistent increase in B8 excitability induced by B40 biased the activity of B8 during feeding motor programs causing the motor programs to become more ingestive in nature. Thus, a single transmitter released from a single interneuron can have consequences for motor output that are expressed over multiple time scales. Importantly, despite the differences in their signs and temporal characteristics, the three actions of B40 are coherent in that they promote B8 firing patterns that are characteristic of ingestive motor outputs.

  1. Time scales and mechanisms of relaxation in the energy landscape of polymer glass under deformation: direct atomistic modeling. (United States)

    Lyulin, Alexey V; Michels, M A J


    Molecular-dynamics simulation is used to explore the influence of thermal and mechanical history of typical glassy polymers on their deformation. Polymer stress-strain and energy-strain developments have been followed for different deformation velocities, also in closed extension-recompression loops. The latter simulate for the first time the experimentally observed mechanical rejuvenation and overaging of polymers, and energy partitioning reveals essential differences between mechanical and thermal rejuvenation. All results can be qualitatively interpreted by considering the ratios of the relevant time scales: for cooling down, for deformation, and for segmental relaxation.

  2. The unusual optical afterglow of the gamma-ray burst GRB 021004 Color changes and short-time-scale variability

    CERN Document Server

    Bersier, D F; Winn, J N; Grav, T; Holman, M J; Matheson, T; Mochejska, B; Steeghs, D; Walker, A R; Garnavich, P M; Quinn, J; Jha, S; Calitz, H; Meintjes, P


    We report UBVRI observations of the optical afterglow of the gamma-ray burst GRB 021004. We observed significant (10-20%) deviations from a power law decay on several time scales, ranging from a few hours down to 20-30 minutes. We also observed a significant color change starting ~1.5 days after the burst, confirming the spectroscopic results already reported by Matheson et al. (2002). We discuss these results in the context of several models that have recently been proposed to account for the anomalous photometric behavior of this event.

  3. Characteristic time scales of coalescence of silver nanocomposite and nanoparticle films induced by continuous wave laser irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Paeng, Dongwoo; Grigoropoulos, Costas P., E-mail: [Department of Mechanical Engineering, University of California Berkeley, Berkeley, California 94720-1740 (United States); Lee, Daeho [Department of Mechanical Engineering, Gachon University, Seongnam-si, Gyeonggi-do 461-701 (Korea, Republic of)


    In-situ optical probing has been performed to analyze and compare the characteristic coalescence time scales of silver ion-doped polyvinylalcohol nanocomposite (Ag-PVA NC) and polyvinylpyrrolidone-capped silver nanoparticle (Ag-PVP NP) films subjected to continuous wave laser irradiation. The Ag-PVA NC yielded conductive metallic patterns by photothermal reduction of PVA, formation of nanoparticles from silver ions and their subsequent coalescence. On the other hand, Ag-PVP NP thin films produced conductive patterns through only coalescence of nanoparticles. Upon laser irradiation, Ag-PVA NC and Ag-PVP NP films exhibited different coalescence characteristics.

  4. Antimicrobial Peptides in 2014

    Directory of Open Access Journals (Sweden)

    Guangshun Wang


    Full Text Available This article highlights new members, novel mechanisms of action, new functions, and interesting applications of antimicrobial peptides reported in 2014. As of December 2014, over 100 new peptides were registered into the Antimicrobial Peptide Database, increasing the total number of entries to 2493. Unique antimicrobial peptides have been identified from marine bacteria, fungi, and plants. Environmental conditions clearly influence peptide activity or function. Human α-defensin HD-6 is only antimicrobial under reduced conditions. The pH-dependent oligomerization of human cathelicidin LL-37 is linked to double-stranded RNA delivery to endosomes, where the acidic pH triggers the dissociation of the peptide aggregate to release its cargo. Proline-rich peptides, previously known to bind to heat shock proteins, are shown to inhibit protein synthesis. A model antimicrobial peptide is demonstrated to have multiple hits on bacteria, including surface protein delocalization. While cell surface modification to decrease cationic peptide binding is a recognized resistance mechanism for pathogenic bacteria, it is also used as a survival strategy for commensal bacteria. The year 2014 also witnessed continued efforts in exploiting potential applications of antimicrobial peptides. We highlight 3D structure-based design of peptide antimicrobials and vaccines, surface coating, delivery systems, and microbial detection devices involving antimicrobial peptides. The 2014 results also support that combination therapy is preferred over monotherapy in treating biofilms.

  5. Peptide Nucleic Acids (PNA)

    DEFF Research Database (Denmark)


    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  6. Peptide Nucleic Acids

    DEFF Research Database (Denmark)


    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  7. Peptide Nucleic Acids

    DEFF Research Database (Denmark)


    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  8. Peptide Nucleic Acid Synthons

    DEFF Research Database (Denmark)


    A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker....

  9. Peptide-Carrier Conjugation

    DEFF Research Database (Denmark)

    Hansen, Paul Robert


    To produce antibodies against synthetic peptides it is necessary to couple them to a protein carrier. This chapter provides a nonspecialist overview of peptide-carrier conjugation. Furthermore, a protocol for coupling cysteine-containing peptides to bovine serum albumin is outlined....

  10. PH dependent adhesive peptides (United States)

    Tomich, John; Iwamoto, Takeo; Shen, Xinchun; Sun, Xiuzhi Susan


    A novel peptide adhesive motif is described that requires no receptor or cross-links to achieve maximal adhesive strength. Several peptides with different degrees of adhesive strength have been designed and synthesized using solid phase chemistries. All peptides contain a common hydrophobic core sequence flanked by positively or negatively charged amino acids sequences.

  11. First isolation of an antifungal lipid transfer peptide from seeds of a Brassica species. (United States)

    Lin, Peng; Xia, Lixin; Ng, T B


    An antifungal peptide with a molecular mass of 9412 and an N-terminal sequence exhibiting notable homology to those of lipid transfer proteins was isolated from seeds of the vegetable Brassica campestris. The purification protocol entailed ion exchange chromatography on Q-Sepharose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography by fast protein liquid chromatography (FPLC) on Mono S, and gel filtration by FPLC on a Superdex peptide column. The antifungal peptide was adsorbed on Affi-gel blue gel and Mono S. It inhibited mycelial growth in Fusarium oxysporum and Mycosphaerella arachidicola with an IC(50) value of 8.3 microM and 4.5 microM, respectively. It exhibited dose-dependent binding to lyso-alpha-lauroyl phosphatidylcholine. The present findings constitute the first report on a non-specific lipid transfer protein from the seeds of a Brassica species.

  12. A peptide with potent antifungal and antiproliferative activities from Nepalese large red beans. (United States)

    Ma, D Z; Wang, H X; Ng, T B


    An antifungal defensin-like peptide with a molecular mass of 7.1kDa was isolated from dried Nepalese large red beans (Phaseolus angularis). The purification protocol employed included ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-Sepharose, and gel filtration by fast protein liquid chromatography on Superdex 75. The antifungal peptide was unadsorbed on DEAE-cellulose, and adsorbed on Affi-gel blue gel and SP-Sepharose. The antifungal peptide inhibited mycelial growth in Fusarium oxysporum and Mycosphaerella arachidicola with an IC(50) value of 1.4 and 1.8 microM, respectively. It did not inhibit HIV-1 reverse transcriptase when tested up to 200 microM. It exerted an antiproliferative action on L1210 leukemia cells and MBL2 lymphoma cells with an IC(50) of 15 and 60 microM, respectively.

  13. Sub-Daily Runoff Simulations with Parameters Inferred at the Daily Time Scale: Impacts of the temporal distribution of rainfall in parameter inference. (United States)

    Reynolds Puga, Jose Eduardo; Halldin, Sven; Xu, Chong-Yu; Seibert, Jan


    Flood forecasting at sub-daily time scales are commonly required in regions where sub-daily observational data are not available. This has led to approaches to estimate model parameters at sub-daily time scales from data with a lower time resolution. Reynolds et al. (2015) show that parameters inferred at one time scale (e.g., daily) may be used directly for runoff simulations at other time scales (e.g., 1 h) when the modelling time step is the same and sufficiently small during calibration and simulation periods. Their approach produced parameter distributions at daily and sub-daily time scales that were similar and relatively constant across the time scales. The transfer of parameter values across time scales resulted in small model-performance decrease as opposed to when the parameter sets inferred at their respective time scale were used. This decrease in performance may be attributed to the degree of information lost, in terms of the physical processes occurring at short time scales, when the rainfall-runoff data used during the parameter-inference phase become coarser. It is not yet fully understood how the aggregation (or disaggregation) of the rainfall-runoff data affects parameter inference. In this study we analyse the impacts of the temporal distribution of rainfall for inferring model parameters at a coarse time scale and their effects in model performance when they are used at finer time scales, where data may not be available for calibration. The motivation is to improve runoff predictions and model performance at sub-daily time scales when parameters inferred at the daily scale are used for simulating at these scales. First, we calibrated the HBV-light conceptual hydrological model at the daily scale, but modelled discharge internally in 1-h time steps using 3 disaggregation procedures of the rainfall data. This was done in an attempt to maximise the information content of the input data used for calibration at the daily scale. One disaggregation

  14. A New Amide Proton R{sub 1{rho}} Experiment Permits Accurate Characterization of Microsecond Time-scale Conformational Exchange

    Energy Technology Data Exchange (ETDEWEB)

    Eichmueller, Christian; Skrynnikov, Nikolai R. [Purdue University, Department of Chemistry (United States)], E-mail:


    A new off-resonance spin-lock experiment to record relaxation dispersion profiles of amide protons is presented. The sensitivity-enhanced HSQC-type sequence is designed to minimize the interference from cross-relaxation effects and ensure that the dispersion profiles in the absence of {mu}s-ms time-scale dynamics are flat. Toward this end (i) the proton background is eliminated by sample deuteration (Ishima et al., 1998), (ii) {sup 1}H spin lock is applied to two-spin modes 2(H{sub x}Sin {theta} + H{sub z}Cos {theta})N{sub z}, and (iii) the tilt angle {theta} {approx} 35 deg. is maintained throughout the series of measurements (Desvaux et al. Mol. Phys., 86 (1995) 1059). The relaxation dispersion profiles recorded in this manner sample a wide range of effective rf field strengths (up to and in excess of 20 kHz) which makes them particularly suitable for studies of motions on the time scale {<=}100 {mu}s. The new experiment has been tested on the Ca{sup 2+}-loaded regulatory domain of cardiac troponin C. Many residues show pronounced dispersions with remarkably similar correlation times of 30 {mu}s. Furthermore, these residues are localized in the regions that have been previously implicated in conformational changes (Spyracopoulos et al. Biochemistry, 36 (1997) 12138)

  15. Dispersion analysis of Lamb waves and damage detection for aluminum structures using ridge in the time-scale domain (United States)

    Li, Fucai; Meng, Guang; Ye, Lin; Lu, Ye; Kageyama, Kazuro


    In this paper, the dispersion of Lamb waves in aluminum structures was systematically analyzed to differentiate the mode of each package in Lamb wave signals and localize damage. Piezoelectric transducers were bonded on the surfaces of aluminum structures, functioning as actuator and sensor to excite and acquire Lamb waves, respectively. Wavelet transform was applied to the acquired Lamb wave signals, in which the optimal mother wavelet was selected using the concept of Shannon entropy to obtain the most accurate location of each wave package. The ridge and contour of the Lamb wave signals in the time-scale domain were obtained to distinguish the mode of each wave package and pinpoint these packages for estimating the actual group velocities of dispersion curves and localizing damage. The proposed approach could help search the actual dispersion curves in the excitation frequency band by using only one Lamb wave signal. Ridges in the time-scale domain and the actual group velocities were further used to identify damage in the structures. Results demonstrate that the proposed approaches were effective in dispersion analysis, wave mode differentiation and damage localization.

  16. A robust computational technique for model order reduction of two-time-scale discrete systems via genetic algorithms. (United States)

    Alsmadi, Othman M K; Abo-Hammour, Zaer S


    A robust computational technique for model order reduction (MOR) of multi-time-scale discrete systems (single input single output (SISO) and multi-input multioutput (MIMO)) is presented in this paper. This work is motivated by the singular perturbation of multi-time-scale systems where some specific dynamics may not have significant influence on the overall system behavior. The new approach is proposed using genetic algorithms (GA) with the advantage of obtaining a reduced order model, maintaining the exact dominant dynamics in the reduced order, and minimizing the steady state error. The reduction process is performed by obtaining an upper triangular transformed matrix of the system state matrix defined in state space representation along with the elements of B, C, and D matrices. The GA computational procedure is based on maximizing the fitness function corresponding to the response deviation between the full and reduced order models. The proposed computational intelligence MOR method is compared to recently published work on MOR techniques where simulation results show the potential and advantages of the new approach.

  17. Dynamics of condensate formation in stochastic transport with pair-factorized steady states: Nucleation and coarsening time scales (United States)

    Nagel, Hannes; Janke, Wolfhard


    Driven diffusive systems such as the zero-range process (ZRP) and the pair-factorized steady states (PFSS) stochastic transport process are versatile tools that lend themselves to the study of transport phenomena on a generic level. While their mathematical structure is simple enough to allow significant analytical treatment, they offer a variety of interesting phenomena. With appropriate dynamics, the ZRP and PFSS models feature a condensation transition where, for a supercritical density, the translational symmetry breaks spontaneously and excess particles form a single-site or spatially extended condensate, respectively. In this paper we numerically study the typical time scales of the two stages of this condensation process: Nucleation and coarsening. Nucleation is the first stage of condensation where the bulk system relaxes to its stationary distribution and droplet nuclei form in the system. These droplets then gradually grow or evaporate in the coarsening regime to coalesce in a single condensate when the system finally relaxes to the stationary state. We use the ZRP condensation model to discuss the choice of the estimation method for the nucleation time scale and present scaling exponents for the ZRP and PFSS condensation models with respect to the choice of the typical droplet nuclei mass. We then proceed to present scaling exponents in the coarsening regime of the ZRP for partially asymmetric dynamics and the PFSS model for symmetric and asymmetric dynamics.

  18. Galaxy Merger Morphologies and Time-Scales from Simulations of Equal-Mass Gas-Rich Disc Mergers

    CERN Document Server

    Lotz, Jennifer M; Cox, T J; Primack, Joel R


    A key obstacle to understanding the galaxy merger rate and its role in galaxy evolution is the difficulty in constraining the merger properties and time-scales from instantaneous snapshots of the real universe. The most common way to identify galaxy mergers is by morphology, yet current theoretical calculations of the time-scales for galaxy disturbances are quite crude. We present a morphological analysis of a large suite of GADGET N-Body/hydro-dynamical equal-mass gas-rich disc galaxy mergers which have been processed through the Monte-Carlo radiative transfer code SUNRISE. With the resulting images, we examine the dependence of quantitative morphology (G, M20, C, A) in the SDSS g-band on merger stage, dust, viewing angle, orbital parameters, gas properties, supernova feedback, and total mass. We find that mergers appear most disturbed in G-M20 and asymmetry at the first pass and at the final coalescence of their nuclei, but can have normal quantitative morphologies at other merger stages. The merger observa...

  19. Multiple Time-scale Characteristics of Runoff Variations in Middle Reaches of Huolin River and Their Effects

    Institute of Scientific and Technical Information of China (English)

    LU Xiaoning; DENG Wei; ZHANG Shuqing


    The Huolin River is one of the most important water sources for Xianghai wetland, Horqin wetland, and Chaganhu wetland in the western Songnen Plain of Northeast China. The annual runoff series of 46 years at Baiyunhushuo Hydrologic Station, which is located in the middle reaches of the Huolin River, were analyzed by using wavelet analysis. Main objective was to discuss the periodic characteristics of the runoff, and examine the temporal patterns of the Huolin River recharging to the floodplain wetlands in the lower reaches of the river, and the corresponding effects of recharging variation on the environmental evolution of the wetlands. The results show that the annual runoff varied mainly at three time scales. The intensities of periodical signals at different time scales were strongly characterized by local distribution in its time frequency domain. The interdecadal variation at a scale of more than 30yr played a leading role in the temporal pattern of runoff variation, and at this scale, the runoff at Baiyunhushuo Hydrologic Station varied in turn of flood, draught and flood. Accordingly, the landscape of the floodplain wetlands presented periodic features, especially prominent before the 1990s. Compared with intense human activities, the runoff periodic pattern at middle (10-20yr) and small (l-10yr) scales, which has relatively low energy, exerted unobvious effects on the environmental evolution of the floodplain wetlands, especially after the 1990s.

  20. On the Use of Space-Environmental Satellite Data for Global Magnetohydrodynamic Simulations. Time-Scale Initialisation Approach (United States)

    Lorenzo, Maibys Sierra; Domingues, Margarete Oliveira; Mecías, Angela León; Menconi, Varlei Everton; Mendes, Odim


    A global magnetohydrodynamic (MHD) model describes the solar-terrestrial system and the physical processes that live in it. Information obtained from satellites provides input to MHD model to compose a more realistic initial state for the equations and, therefore, more accurate simulations. However, the use of high resolution in time data can produce numerical instabilities that quickly interrupt the simulations. Moreover, satellite time series may have gaps which could be a problem in this context. In order to contribute to the overcoming of such challenges, we propose in this work a methodology based on a variant of the continuous wavelet transform to introduce environmental satellite data on the global resistive MHD model originally developed by Prof. Ogino at the University of Nagoya. Our methodology uses a simplified time-scale version of the original data that preserves the most important spectral features of the phenomena of interest. Then, we can do a long-term integration using this MHD model without any computational instability, while preserving the main time-scale features of the original data set and even overcome possible occurrence of gaps on the satellite data. This methodology also contributes to keeping more realistic physical results.

  1. Climate impact of beef: an analysis considering multiple time scales and production methods without use of global warming potentials (United States)

    Pierrehumbert, R. T.; Eshel, G.


    An analysis of the climate impact of various forms of beef production is carried out, with a particular eye to the comparison between systems relying primarily on grasses grown in pasture (‘grass-fed’ or ‘pastured’ beef) and systems involving substantial use of manufactured feed requiring significant external inputs in the form of synthetic fertilizer and mechanized agriculture (‘feedlot’ beef). The climate impact is evaluated without employing metrics such as {{CO}}2{{e}} or global warming potentials. The analysis evaluates the impact at all time scales out to 1000 years. It is concluded that certain forms of pastured beef production have substantially lower climate impact than feedlot systems. However, pastured systems that require significant synthetic fertilization, inputs from supplemental feed, or deforestation to create pasture, have substantially greater climate impact at all time scales than the feedlot and dairy-associated systems analyzed. Even the best pastured system analyzed has enough climate impact to justify efforts to limit future growth of beef production, which in any event would be necessary if climate and other ecological concerns were met by a transition to primarily pasture-based systems. Alternate mitigation options are discussed, but barring unforseen technological breakthroughs worldwide consumption at current North American per capita rates appears incompatible with a 2 °C warming target.

  2. A Robust Computational Technique for Model Order Reduction of Two-Time-Scale Discrete Systems via Genetic Algorithms

    Directory of Open Access Journals (Sweden)

    Othman M. K. Alsmadi


    Full Text Available A robust computational technique for model order reduction (MOR of multi-time-scale discrete systems (single input single output (SISO and multi-input multioutput (MIMO is presented in this paper. This work is motivated by the singular perturbation of multi-time-scale systems where some specific dynamics may not have significant influence on the overall system behavior. The new approach is proposed using genetic algorithms (GA with the advantage of obtaining a reduced order model, maintaining the exact dominant dynamics in the reduced order, and minimizing the steady state error. The reduction process is performed by obtaining an upper triangular transformed matrix of the system state matrix defined in state space representation along with the elements of B, C, and D matrices. The GA computational procedure is based on maximizing the fitness function corresponding to the response deviation between the full and reduced order models. The proposed computational intelligence MOR method is compared to recently published work on MOR techniques where simulation results show the potential and advantages of the new approach.

  3. Climatic and human impacts on quasi-periodic and abrupt changes of sedimentation rate at multiple time scales in Lake Taihu, China (United States)

    Liu, Huiyu; Xu, Xiaojuan; Lin, Zhenshan; Zhang, Mingyang; Mi, Ying; Huang, Changchun; Yang, Hao


    With the ensemble Empirical Mode Decomposition Method (EEMD) and the non-parametric Mann-Kendall Test, the quasi-periodic and abrupt changes of sedimentation rate at multiple time scales, and their relations to climate changes and human activities from 1951 to 2010 in Meiliang Bay of Lake Taihu (China) were studied. The results showed the following. (1) The change in sedimentation rate can be completely decomposed into three quasi-periodic changes on 3.7, 6.4, and 24-yr time scales, and a long-term trend. (2) The quasi-periodic changes in sedimentation rate are significantly and positively related to changes in annual average temperature at 6.4 and 24-yr time scales and human activities at 3.7-yr time scales, and not significantly related to precipitation at these time scales. The trend of sedimentation rate has a negative relation with temperature, but positive relations with precipitation and human activities. As a whole, the total variance contribution of climate changes to the quasi-periodic changes of sedimentation rate is close to that of human activities; (3) Temperature and precipitation are possibly related to the abrupt change of sedimentation rate as a whole. Floods have significant impacts on abrupt changes in the sedimentation rate at 3.7, 6.4 and 24-yr time scales. Moreover, some abrupt changes of sedimentation rate at 3.7- and 6.4-yr time scales are partly related to the changes of precipitation at 3.1-yr time scale and temperature at 5-yr time scale. The results of this study will help identify the impacts of climate change and human activities on lake sedimentation at different time scales, and will be available for use as a guide for reasonable development and effective protection of lake resources.

  4. Fun with Paper Chromatography. (United States)

    Coleman, Dava; Hounshell, Paul B.


    Discusses paper chromatographic techniques and provides examples of typical classroom activities. Includes description of retardation values obtained during chromatography exercises and suggests using them for math lessons. (JN)

  5. Three dimensional liquid chromatography coupling ion exchange chromatography/hydrophobic interaction chromatography/reverse phase chromatography for effective protein separation in top-down proteomics. (United States)

    Valeja, Santosh G; Xiu, Lichen; Gregorich, Zachery R; Guner, Huseyin; Jin, Song; Ge, Ying


    To address the complexity of the proteome in mass spectrometry (MS)-based top-down proteomics, multidimensional liquid chromatography (MDLC) strategies that can effectively separate proteins with high resolution and automation are highly desirable. Although various MDLC methods that can effectively separate peptides from protein digests exist, very few MDLC strategies, primarily consisting of 2DLC, are available for intact protein separation, which is insufficient to address the complexity of the proteome. We recently demonstrated that hydrophobic interaction chromatography (HIC) utilizing a MS-compatible salt can provide high resolution separation of intact proteins for top-down proteomics. Herein, we have developed a novel 3DLC strategy by coupling HIC with ion exchange chromatography (IEC) and reverse phase chromatography (RPC) for intact protein separation. We demonstrated that a 3D (IEC-HIC-RPC) approach greatly outperformed the conventional 2D IEC-RPC approach. For the same IEC fraction (out of 35 fractions) from a crude HEK 293 cell lysate, a total of 640 proteins were identified in the 3D approach (corresponding to 201 nonredundant proteins) as compared to 47 in the 2D approach, whereas simply prolonging the gradients in RPC in the 2D approach only led to minimal improvement in protein separation and identifications. Therefore, this novel 3DLC method has great potential for effective separation of intact proteins to achieve deep proteome coverage in top-down proteomics.

  6. Time scales in LISA

    CERN Document Server

    Pireaux, S


    The LISA mission is a space interferometer aiming at the detection of gravitational waves in the [$10^{-4}$,$10^{-1}$] Hz frequency band. In order to reach the gravitational wave detection level, a Time Delay Interferometry (TDI) method must be applied to get rid of (most of) the laser frequency noise and optical bench noise. This TDI analysis is carried out in terms of the coordinate time corresponding to the Barycentric Coordinate Reference System (BCRS), TCB, whereas the data at each of the three LISA stations is recorded in terms of each station proper time. We provide here the required proper time versus BCRS time transformation. We show that the difference in rate of station proper time versus TCB is of the order of $5 10^{-8}$. The difference between station proper times and TCB exhibits an oscillatory trend with a maximum amplitude of about $10^{-3}$ s.

  7. Improved Methods for the Enrichment and Analysis of Glycated Peptides

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Qibin; Schepmoes, Athena A; Brock, Jonathan W; Wu, Si; Moore, Ronald J; Purvine, Samuel O; Baynes, John; Smith, Richard D; Metz, Thomas O


    Non-enzymatic glycation of tissue proteins has important implications in the development of complications of diabetes mellitus. Herein we report improved methods for the enrichment and analysis of glycated peptides using boronate affinity chromatography and electron transfer dissociation mass spectrometry, respectively. The enrichment of glycated peptides was improved by replacing an off-line desalting step with an on-line wash of column-bound glycated peptides using 50 mM ammonium acetate. The analysis of glycated peptides by MS/MS was improved by considering only higher charged (≥3) precursor-ions during data-dependent acquisition, which increased the number of glycated peptide identifications. Similarly, the use of supplemental collisional activation after electron transfer (ETcaD) resulted in more glycated peptide identifications when the MS survey scan was acquired with enhanced resolution. In general, acquiring ETD-MS/MS data at a normal MS survey scan rate, in conjunction with the rejection of both 1+ and 2+ precursor-ions, increased the number of identified glycated peptides relative to ETcaD or the enhanced MS survey scan rate. Finally, an evaluation of trypsin, Arg-C, and Lys-C showed that tryptic digestion of glycated proteins was comparable to digestion with Lys-C and that both were better than Arg-C in terms of the number glycated peptides identified by LC-MS/MS.

  8. Membrane-mediated capillary electrophoresis: interaction of cationic peptides with bicelles. (United States)

    Mills, John O; Holland, Lisa A


    Electrokinetic capillary chromatography is applied to determine the membrane affinity of peptides using both 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) micelles and DHPC/1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) bicelles under controlled conditions. The effect of temperature and the bicelle q value in surface association with cationic peptides is studied. The cationic peptides selected have a well-defined membrane structure (indolicidin), induced secondary structure (melittin, magainin 2), or do not possess classical secondary structure (atrial natriuretic peptide (ANP) 1-28, 4-28, 5-27). Electrokinetic capillary chromatography facilitated by DMPC and DHPC additives provides a rapid means of estimating lipophilicity and screening for peptides that have membrane affinity.

  9. Efficient purification of unique antibodies using peptide affinity-matrix columns

    DEFF Research Database (Denmark)

    Jensen, Liselotte Brix; Riise, Erik; Nielsen, Leif Kofoed


    fragments and had no affinity for other antibodies. Using this peptide matrix MK16 IgG could be purified from cell culture supernatants thereby separating MK16 IgG from bovine IgG normally present in the enriched growth media used for such cells. Investigations of the fine specificity of the ER6.1 peptide......Phage display technology was used to identify peptide ligands with unique specificity for a monoclonal model antibody, MK16, that recognises the human multiple sclerosis associated MHC class II molecule DR2 in complex with a myelin basic protein (MBP)-derived peptide corresponding to residue 85......-99. Several peptide epitopes were identified and all of them recognised specifically MK16. One peptide, ER6.1, was selected and linked to beaded agarose and demonstrated excellent performance as a peptide affinity chromatography matrix. This epitope matrix was efficient in the purification of MK16 Fab...

  10. Atmospheric and Oceanic Response to Southern Ocean Deep Convection Oscillations on Decadal to Centennial Time Scales in Climate Models (United States)

    Martin, T.; Reintges, A.; Park, W.; Latif, M.


    Many current coupled global climate models simulate open ocean deep convection in the Southern Ocean as a recurring event with time scales ranging from a few years to centennial (de Lavergne et al., 2014, Nat. Clim. Ch.). The only observation of such event, however, was the occurrence of the Weddell Polynya in the mid-1970s, an open water area of 350 000 km2 within the Antarctic sea ice in three consecutive winters. Both the wide range of modeled frequency of occurrence and the absence of deep convection in the Weddell Sea highlights the lack of understanding concerning the phenomenon. Nevertheless, simulations indicate that atmospheric and oceanic responses to the cessation of deep convection in the Southern Ocean include a strengthening of the low-level atmospheric circulation over the Southern Ocean (increasing SAM index) and a reduction in the export of Antarctic Bottom Water (AABW), potentially masking the regional effects of global warming (Latif et al., 2013, J. Clim.; Martin et al., 2014, Deep Sea Res. II). It is thus of great importance to enhance our understanding of Southern Ocean deep convection and clarify the associated time scales. In two multi-millennial simulations with the Kiel Climate Model (KCM, ECHAM5 T31 atmosphere & NEMO-LIM2 ~2˚ ocean) we showed that the deep convection is driven by strong oceanic warming at mid-depth periodically overriding the stabilizing effects of precipitation and ice melt (Martin et al., 2013, Clim. Dyn.). Sea ice thickness also affects location and duration of the deep convection. A new control simulation, in which, amongst others, the atmosphere grid resolution is changed to T42 (~2.8˚), yields a faster deep convection flip-flop with a period of 80-100 years and a weaker but still significant global climate response similar to CMIP5 simulations. While model physics seem to affect the time scale and intensity of the phenomenon, the driving mechanism is a rather robust feature. Finally, we compare the atmospheric and

  11. Liquid Chromatography in 1982. (United States)

    Freeman, David H.


    Reviews trends in liquid chromatography including apparatus, factors affecting efficient separation of a mixture (peak sharpness and speed), simplified problem-solving, adsorption, bonded phase chromatography, ion selectivity, and size exclusion. The current trend is to control chemical selectivity by the liquid phase. (Author/JN)

  12. Column Liquid Chromatography. (United States)

    Majors, Ronald E.; And Others


    Reviews literature covering developments of column liquid chromatography during 1982-83. Areas considered include: books and reviews; general theory; columns; instrumentation; detectors; automation and data handling; multidimensional chromatographic and column switching techniques; liquid-solid chromatography; normal bonded-phase, reversed-phase,…

  13. Novel Concepts for Drug Hypersensitivity Based on the Use of Long-Time Scale Molecular Dynamic Simulation (United States)

    Murai, Takahiro; Kawashita, Norihito


    The discovery that several drug hypersensitivity reactions (DHRs) are associated with specific human leukocyte antigen (HLA) alleles has attracted increasing research interest. However, the underlying mechanisms of these HLA-induced DHRs remain unclear, especially for drug-induced immediate activation of T-cell clones (TCCs). Recently, a novel hypothesis involving partial detachment between self-peptide(s) and the HLA molecule (altered peptide-HLA (pHLA) model) has been proposed to explain these phenomena. In order to clarify this hypothesis, we performed long-timescale molecular dynamics (MD) simulations. We focused on HLA-B⁎57:01-restricted abacavir hypersensitivity reactions (AHRs), one of the most famous DHRs. One of the simulation results showed that this altered-pHLA model might be driven by an increase in the distance not only between HLA and self-peptides but also between the α1 and α2 helices of HLA. Our findings provide novel insights into abacavir-induced immediate activation of TCCs and these findings might also be applied to other DHRs, such as HLA-B⁎58:01-restricted allopurinol hypersensitivity reactions. PMID:27999707

  14. Novel Concepts for Drug Hypersensitivity Based on the Use of Long-Time Scale Molecular Dynamic Simulation

    Directory of Open Access Journals (Sweden)

    Takahiro Murai


    Full Text Available The discovery that several drug hypersensitivity reactions (DHRs are associated with specific human leukocyte antigen (HLA alleles has attracted increasing research interest. However, the underlying mechanisms of these HLA-induced DHRs remain unclear, especially for drug-induced immediate activation of T-cell clones (TCCs. Recently, a novel hypothesis involving partial detachment between self-peptide(s and the HLA molecule (altered peptide-HLA (pHLA model has been proposed to explain these phenomena. In order to clarify this hypothesis, we performed long-timescale molecular dynamics (MD simulations. We focused on HLA-B⁎57:01-restricted abacavir hypersensitivity reactions (AHRs, one of the most famous DHRs. One of the simulation results showed that this altered-pHLA model might be driven by an increase in the distance not only between HLA and self-peptides but also between the α1 and α2 helices of HLA. Our findings provide novel insights into abacavir-induced immediate activation of TCCs and these findings might also be applied to other DHRs, such as HLA-B⁎58:01-restricted allopurinol hypersensitivity reactions.

  15. Periodic Solutions for a Semi-Ratio-Dependent Predator-Prey System with Delays on Time Scales

    Directory of Open Access Journals (Sweden)

    Xiaoquan Ding


    Full Text Available This paper is devoted to the existence of periodic solutions for a semi-ratio-dependent predator-prey system with time delays on time scales. With the help of a continuation theorem based on coincidence degree theory, we establish necessary and sufficient conditions for the existence of periodic solutions. Our results show that for the most monotonic prey growth such as the logistic, the Gilpin, and the Smith growth, and the most celebrated functional responses such as the Holling type, the sigmoidal type, the Ivlev type, the Monod-Haldane type, and the Beddington-DeAngelis type, the system always has at least one periodic solution. Some known results are shown to be special cases of the present paper.

  16. Eddy-correlation measurements of benthic fluxes under complex flow conditions: Effects of coordinate transformations and averaging time scales

    DEFF Research Database (Denmark)

    Lorke, Andreas; McGinnis, Daniel F.; Maeck, Andreas


    hours of continuous eddy-correlation measurements of sediment oxygen fluxes in an impounded river, we demonstrate that rotation of measured current velocities into streamline coordinates can be a crucial and necessary step in data processing under complex flow conditions in non-flat environments......Eddy-correlation measurements of sediment oxygen uptake rates in aquatic systems are increasingly used to obtain areal-averaged fluxes with a high temporal resolution. Here we discuss the effects of coordinate rotation and averaging time scale for Reynolds decomposition on flux estimates. Using 119...... in the context of the theoretical concepts underlying eddy-correlation measurements and a set of recommendations for planning and analyses of flux measurements are derived....

  17. New theoretical approaches to atomic and molecular dynamics triggered by ultrashort light pulses on the atto- to picosecond time scale

    Energy Technology Data Exchange (ETDEWEB)

    Pabst, Stefan Ulf


    The concept of atoms as the building blocks of matter has existed for over 3000 years. A revolution in the understanding and the description of atoms and molecules has occurred in the last century with the birth of quantum mechanics. After the electronic structure was understood, interest in studying the dynamics of electrons, atoms, and molecules increased. However, time-resolved investigations of these ultrafast processes were not possible until recently. The typical time scale of atomic and molecular processes is in the picosecond to attosecond realm. Tremendous technological progress in recent years makes it possible to generate light pulses on these time scales. With such ultrashort pulses, atomic and molecular dynamics can be triggered, watched, and controlled. Simultaneously, the need rises for theoretical models describing the underlying mechanisms. This doctoral thesis focuses on the development of theoretical models which can be used to study the dynamical behavior of electrons, atoms, and molecules in the presence of ultrashort light pulses. Several examples are discussed illustrating how light pulses can trigger and control electronic, atomic, and molecular motions. In the first part of this work, I focus on the rotational motion of asymmetric molecules, which happens on picosecond and femtosecond time scales. Here, the aim is to align all three axes of the molecule as well as possible. To investigate theoretically alignment dynamics, I developed a program that can describe alignment motion ranging from the impulsive to the adiabatic regime. The asymmetric molecule SO{sub 2} is taken as an example to discuss strategies of optimizing 3D alignment without the presence of an external field (i.e., field-free alignment). Field-free alignment is particularly advantageous because subsequent experiments on the aligned molecule are not perturbed by the aligning light pulse. Wellaligned molecules in the gas phase are suitable for diffraction experiments. From the

  18. Towards a Seamless Framework for Drought Analysis and Prediction from Seasonal to Climate Change Time Scales (Plinius Medal Lecture) (United States)

    Sheffield, Justin


    Droughts arguably cause the most impacts of all natural hazards in terms of the number of people affected and the long-term economic costs and ecosystem stresses. Recent droughts worldwide have caused humanitarian and economic problems such as food insecurity across the Horn of Africa, agricultural economic losses across the central US and loss of livelihoods in rural western India. The prospect of future increases in drought severity and duration driven by projected changes in precipitation patterns and increasing temperatures is worrisome. Some evidence for climate change impacts on drought is already being seen for some regions, such as the Mediterranean and east Africa. Mitigation of the impacts of drought requires advance warning of developing conditions and enactment of drought plans to reduce vulnerability. A key element of this is a drought early warning system that at its heart is the capability to monitor evolving hydrological conditions and water resources storage, and provide reliable and robust predictions out to several months, as well as the capacity to act on this information. At longer time scales, planning and policy-making need to consider the potential impacts of climate change and its impact on drought risk, and do this within the context of natural climate variability, which is likely to dominate any climate change signal over the next few decades. There are several challenges that need to be met to advance our capability to provide both early warning at seasonal time scales and risk assessment under climate change, regionally and globally. Advancing our understanding of drought predictability and risk requires knowledge of drought at all time scales. This includes understanding of past drought occurrence, from the paleoclimate record to the recent past, and understanding of drought mechanisms, from initiation, through persistence to recovery and translation of this understanding to predictive models. Current approaches to monitoring and

  19. Several Existence Theorems of Multiple Positive Solutions of Nonlinear -Point BVP for an Increasing Homeomorphism and Homomorphism on Time Scales

    Directory of Open Access Journals (Sweden)

    Kang Shugui


    Full Text Available Abstract By using fixed point theorems in cones, the existence of multiple positive solutions is considered for nonlinear -point boundary value problem for the following second-order boundary value problem on time scales , , , , where is an increasing homeomorphism and homomorphism and . Some new results are obtained for the existence of twin or an arbitrary odd number of positive solutions of the above problem by applying Avery-Henderson and Leggett-Williams fixed point theorems, respectively. In particular, our criteria generalize and improve some known results by Ma and Castaneda (2001. We must point out for readers that there is only the -Laplacian case for increasing homeomorphism and homomorphism. As an application, one example to demonstrate our results is given.

  20. Coherent motions and time scales that control heat and mass transfer at wind-swept water surfaces (United States)

    Turney, D. E.


    Forecast of the heat and chemical budgets of lakes, rivers, and oceans requires improved predictive understanding of air-water interfacial transfer coefficients. Here we present laboratory observations of the coherent motions that occupy the air-water interface at wind speeds (U10) 1.1-8.9 m/s. Spatiotemporal near-surface velocity data and interfacial renewal data are made available by a novel flow tracer method. The relative activity, velocity scales, and time scales of the various coherent interfacial motions are measured, namely for Langmuir circulations, streamwise streaks, nonbreaking wind waves, parasitic capillary waves, nonturbulent breaking wind waves, and turbulence-generating breaking wind waves. Breaking waves exhibit a sudden jump in streamwise interfacial velocity wherein the velocity jumps up to exceed the wave celerity and destroys nearby parasitic capillary waves. Four distinct hydrodynamic regimes are found to exist between U10 = 0 and 8.9 m/s, each with a unique population balance of the various coherent motions. The velocity scales, time scales, and population balance of the different coherent motions are input to a first-principles gas transfer model to explain the waterside transfer coefficient (kw) as well as experimental patterns of temperature and gas concentration. The model mixes concepts from surface renewal and divergence theories and requires surface divergence strength (β), the Lagrangian residence time inside the upwelling zone (tLu), and the total lifetime of new interface before it is downwelled (tLT). The model's output agrees with time-averaged measurements kw, patterns of temperature in infrared photographs, and spatial patterns of gas concentration and kw from direct numerical simulations. Several nondimensional parameters, e.g. βtLu and τstLT where τs is the interfacial shear rate, determine the effectiveness of a particular type of coherent motion for affecting kw.