WorldWideScience

Sample records for chorion

  1. -Human Chorionic Gonadotropin Production

    Directory of Open Access Journals (Sweden)

    Michael J. Russell

    2008-01-01

    Full Text Available Dedifferentiated liposarcomas may display a variety of “heterologous” lines of differentiation, including osseous, vascular, skeletal, and/or smooth muscular. There have been six previously reported examples of leiomyosarcomas associated with high levels of serum human chorionic gonadotropin (hCG production, comprised of cases originating from the retroperitoneum, spermatic cord, small intestine, and uterus. This report describes the first example of a dedifferentiated liposarcoma that combined both of the aforementioned features: extensive heterologous (leiomyosarcomatous differentiation and -hCG production (maximum serum levels 1046 mIU/ml, reference <5 mIU/ml. The tumor, which originated in the retroperitoneum in the region of the right kidney, was rapidly progressive and ultimately fatal within three months of its diagnosis. In addition to characteristic morphologic features, lipogenic and smooth muscle differentiation were confirmed with immunohistochemical stains for MDM2 and smooth muscle actin, respectively. The tumor also displayed diffuse immunoreactivity for -hCG in both primary and metastatic sites. This case further expands the clinicopathologic spectrum of lipogenic tumors.

  2. A comprehensive analysis of the chorion locus in silkmoth.

    Science.gov (United States)

    Chen, Zhiwei; Nohata, Junko; Guo, Huizhen; Li, Shenglong; Liu, Jianqiu; Guo, Youbing; Yamamoto, Kimiko; Kadono-Okuda, Keiko; Liu, Chun; Arunkumar, Kallare P; Nagaraju, Javaregowda; Zhang, Yan; Liu, Shiping; Labropoulou, Vassiliki; Swevers, Luc; Tsitoura, Panagiota; Iatrou, Kostas; Gopinathan, Karumathil P; Goldsmith, Marian R; Xia, Qingyou; Mita, Kazuei

    2015-11-10

    Despite more than 40 years of intense study, essential features of the silkmoth chorion (eggshell) are still not fully understood. To determine the precise structure of the chorion locus, we performed extensive EST analysis, constructed a bacterial artificial chromosome (BAC) contig, and obtained a continuous genomic sequence of 871,711 base pairs. We annotated 127 chorion genes in two segments interrupted by a 164 kb region with 5 non-chorion genes, orthologs of which were on chorion bearing scaffolds in 4 ditrysian families. Detailed transcriptome analysis revealed expression throughout choriogenesis of most chorion genes originally categorized as "middle", and evidence for diverse regulatory mechanisms including cis-elements, alternative splicing and promoter utilization, and antisense RNA. Phylogenetic analysis revealed multigene family associations and faster evolution of early chorion genes and transcriptionally active pseudogenes. Proteomics analysis identified 99 chorion proteins in the eggshell and micropyle localization of 1 early and 6 Hc chorion proteins.

  3. LepChorionDB, a database of Lepidopteran chorion proteins and a set of tools useful for the identification of chorion proteins in Lepidopteran proteomes.

    Science.gov (United States)

    Giannopoulos, Nikolaos G; Michalopoulos, Ioannis; Papandreou, Nikos C; Malatras, Apostolos; Iconomidou, Vassiliki A; Hamodrakas, Stavros J

    2013-02-01

    Chorion proteins of Lepidoptera have a tripartite structure, which consists of a central domain and two, more variable, flanking arms. The central domain is highly conserved and it is used for the classification of chorion proteins into two major classes, A and B. Annotated and unreviewed Lepidopteran chorion protein sequences are available in various databases. A database, named LepChorionDB, was constructed by searching 5 different protein databases using class A and B central domain-specific profile Hidden Markov Models (pHMMs), developed in this work. A total of 413 Lepidopteran chorion proteins from 9 moths and 1 butterfly species were retrieved. These data were enriched and organised in order to populate LepChorionDB, the first relational database, available on the web, containing Lepidopteran chorion proteins grouped in A and B classes. LepChorionDB may provide insights in future functional and evolutionary studies of Lepidopteran chorion proteins and thus, it will be a useful tool for the Lepidopteran scientific community and Lepidopteran genome annotators, since it also provides access to the two pHMMs developed in this work, which may be used to discriminate A and B class chorion proteins. LepChorionDB is freely available at http://bioinformatics.biol.uoa.gr/LepChorionDB.

  4. Prenatal diagnosis of chorionicity in twins.

    LENUS (Irish Health Repository)

    Hassan, T

    2012-02-01

    The aim of this audit was to assess the accuracy of transabdominal ultrasound scan in predicting chorionicity in twin pregnancies in our unit. The presence or absence of lambda sign, T-sign, dividing membrane thickness and number of placentae were used to determine chorionicity. We retrospectively analysed these antenatal markers in 268 sets of twins delivered over a 5 year period and compared it with the postpartum placental histology and neonatal gender. Of 268 twin deliveries, 204 (76%) had both chorionicity and placental histology to compare. 67 of 84 (80%) were correctly diagnosed antenatally as monochorionic and 137 of 151 (91%) as dichorionic. In 31 cases (15%) the ultrasound diagnosis of chorionicity didn\\'t match placental histology. Seventeen were thought to be monochorionic antenatally but were confirmed dichorionic on placental histology. Overall chorionicity was correctly diagnosed in 171\\/204 (84%) using transabdominal ultrasound scan (USS) in all trimesters. However the sensitivity and specificity of USS was much higher for dichorionic twins when carried out before 14 weeks of gestation.

  5. The Prenatal Environment in Twin Studies: A Review on Chorionicity

    OpenAIRE

    Marceau, Kristine; McMaster, Minni T. B.; Smith, Taylor F.; Daams, Joost G.; van Beijsterveldt, Catharina E.M.; Boomsma, Dorret I.; Valerie S. Knopik

    2016-01-01

    A literature search was conducted to identify articles examining the association of chorionicity (e.g., whether twins share a single chorion and thus placenta or have separate chorions/placentas) and genetics, psychiatry/behavior, and neurological manifestations in humans twins and higher-order multiples. The main aim was to assess how frequently chorionicity has been examined in relation to heritability estimates, and to assess which phenotypes may be most sensitive to, or affected by, bias ...

  6. Candida Sepsis Following Transcervical Chorionic Villi Sampling

    OpenAIRE

    Israel Potasman; Roni Gonen; Alona Paz

    2001-01-01

    Background: The use of invasive devices and broad spectrum antibiotics has increased the rate of candidal superinfections.Candida sepsis associated with pregnancy is rare. Candida sepsis following chorionic villi sampling (CVS) has never been reported. Case: A 31-year-old pregnant woman presented with signs of sepsis one day after undergoing transcervical CVS. Blood culture and curettage material yielded C. albicans. She was treated with 400 mg of fluconazole daily for 4 weeks and completely ...

  7. Transabdominal chorion villus biopsi ved abnormt ultralydfund i 2. trimester

    DEFF Research Database (Denmark)

    Hertz, Jens Michael; Jensen, P K; Henriques, U;

    1990-01-01

    . Three patients with abnormal ultrasonic findings in the second trimester were, therefore, submitted to transabdominal chorion villus biopsy and, in all three cases, a karyotype was available within 48 hours. Chorion villus biopsy in the second (and third) trimester is indicated in pregnancies in which...

  8. The Prenatal Environment in Twin Studies: A Review on Chorionicity.

    Science.gov (United States)

    Marceau, Kristine; McMaster, Minni T B; Smith, Taylor F; Daams, Joost G; van Beijsterveldt, Catharina E M; Boomsma, Dorret I; Knopik, Valerie S

    2016-05-01

    A literature search was conducted to identify articles examining the association of chorionicity (e.g., whether twins share a single chorion and thus placenta or have separate chorions/placentas) and genetics, psychiatry/behavior, and neurological manifestations in humans twins and higher-order multiples. The main aim was to assess how frequently chorionicity has been examined in relation to heritability estimates, and to assess which phenotypes may be most sensitive to, or affected by, bias in heritability estimates because of chorionicity. Consistent with the theory that some chorionicity effects could lead to overestimation and others to underestimation of heritability, there were instances of each across the many phenotypes reviewed. However, firm conclusions should not be drawn since some of the outcomes were only examined in one or few studies and often sample sizes were small. While the evidence for bias due to chorionicity was mixed or null for many outcomes, results do, however, consistently suggest that heritability estimates are underestimated for measures of birth weight and early growth when chorionicity is not taken into account. PMID:26944881

  9. Fertility Rates of Ewes Treated with Medroxyprogesterone and Injected with Equine Chorionic Gonadotropin plus Human Chorionic Gonadotropin in Anoestrous Season

    OpenAIRE

    I.W. Santos; Binsfeld, L. C.; Weiss, R. R.; Kozicki, L. E.

    2010-01-01

    The aim of the present paper was to investigate the efficiency of the equine chorionic gonadotropin (eCG) plus human chorionic gonadotropin (hCG) associated with medroxyprogesterone acetate (MAP) to estrous ewes synchronization. Ninety Texel ewes were investigated during seasonal anoestrous. The ewes received intravaginal sponges containing MAP (60 mg) for nine days. At the time of sponges' withdrawal, the ewes were divided into three groups (G): (1) receiving 2 mL of saline i.m. (n = 30), (2...

  10. Four years' cytogenetic experience with the culture of chorionic villi

    NARCIS (Netherlands)

    Sikkema-Raddatz, B; Bouman, K; Verschuuren-Bemelmans, CC; Stoepker, M; Mantingh, A; Beekhuis, [No Value; de Jong, B

    2000-01-01

    In 1958 chorionic villus samples, investigated by culture method, we found 137 (7%) abnormalities. The abnormal results were classified in certain abnormal (generalised abnormal at high probability) and uncertain abnormal (potentially confined to the placenta) results. Certain abnormal were 73 cases

  11. OBSERVATIONS ON VASCULAR PATTERN OF CHORIONIC BLOOD VESSELS OF PLACENTA

    Directory of Open Access Journals (Sweden)

    Yousuf Sarwar

    2013-10-01

    Full Text Available ABSTRACT: BACKGROUND: Placenta is a choriodecidual structure develops during pregnancy implanted on the uterine wall and car ries vital functions. It is connected to the foetus through umbilical cord. The branches of umbilical vessels that traverse along foetal surface of placenta are referred as chorionic vessels. There are two different patterns of chorionic vessels – Dispersa l and Magistral. In the dispersal type, the umbilical vessels undergo successive divisions with gradually diminishing caliber towards periphery while in magistral pattern the vessels traverse to the edge of placenta without appreciable decrease in diameter of vessels. The present study has been done for visualization of the pattern of chorionic vessels in placenta obtained from labour room of a tertiary care hospital in eastern Bihar, India. MATERIALS AND METHODS: A total of one hundred and fifty (150 fres h and intact placenta of full term pregnancies collected from Obstetrics & Gynaecology department were included in the study. After washing with distilled water, removal of blood clots were done with slight digital pressure applied over arteries and vein a nd later on by irrigation with saline. The cut end of the umbilical cord was carefully visualized to identify the umbilical arteries and vein. Dye was injected into umbilical vessels under normal physiological pressure and diameter of chorionic blood vesse ls were taken at the center and periphery. Ultimately each vessel was followed and examined to observe the dispersal and magistral pattern of chorionic blood vessels of placenta. RESULTS AND CONCLUSION: Out of the total of one hundred and fifty (150 full term placenta obtained and examined in the Anatomy department during the study period, 64% Dispersal type & 36 % of Magistral type of arterial pattern of branching of chorionic vessels were observed. Vein and its tributaries presented Dispersal pattern in 60% and Magistral pattern in 40%. There is a significant

  12. Feasibility and safety of transabdominal chorionic villus sampling

    International Nuclear Information System (INIS)

    Background: Chorionic Villus Sampling (CVS) is the technique of choice for prenatal diagnosis prior to 12 weeks gestation. The objective of this study was to determine the feasibility, and pattern of complications following first trimester Trans-abdominal Chorionic Villus Sampling (TA-CVS). Methods: This was a descriptive study conducted in the Obstetrics and Gynaecology Department Military Hospital (MH) Rawalpindi from Jan 2007 to July 2008. Couples at risk of giving birth to a child with genetic disorder were identified and counselled. Trans-abdominal Chorionic Villus Sampling was done using double needle technique under ultrasound guidance. Immediate and late complications were followed up. Data was analysed using SPPS-10. Results: On 200 cases chorionic villus sampling was done as an outdoor procedure. Most common indication was thalassaemia trait 75 (37.5%). Most procedures were done between 12-13 weeks. All placental positions including 104 (52%) posterior and 71 (35.5%) anterior were approachable. Most aspirations were easy, however, in 30 (15%) the aspiration was difficult. Overall success rate was 100%. In 158 (79%) of the cases sample yield was good. One (0.5%) patient had vaginal bleeding and three (1.5%) had placental haematoma formation. Most patients (84%) experienced mild pain during the procedure. The procedure related miscarriage occurred in 2 (1%) patients while another patient developed this complication after 6 weeks. Conclusion: First trimester TA-CVS is an accurate and safe invasive prenatal diagnostic procedure. Placentas in almost any position can be approached without any significant risk to mother and the foetus. (author)

  13. Prenatal Diagnosis by Amniocentesis and Chorionic Villus Biopsy

    OpenAIRE

    Reynolds, J.L.

    1986-01-01

    Prenatal diagnosis forms only a small part of day-to-day family practice, but the techniques are of critical importance to couples at risk of having a child affected by genetic disorder. Second trimester amniocentesis will probably be replaced by first trimester chorionic villus biopsy and recombinant DNA technology, but the ethical and moral problems related to prenatal diagnosis are not so easily solved. Family physicians need to examine their own attitudes toward the handicapped before the...

  14. Beta-Subunit of Human Chorionic Gonadotropin in Malignant Lymphoma : An Immunohistochemical Study

    OpenAIRE

    Senba, Masachika; Watanabe, Masami

    1991-01-01

    We present a rare case of a 77-year-old Japanese man with malignant lymphoma associated with production of beta-subunit of human chorionic gonadotropin in the cytoplasms of lymphoma cells in the lymph nodes. By immunoperoxidase staining, numerous tumor cells were reacted with beta-subunit of human chorionic gonadotropin. To the best of our knowledge, production of beta-subunit of human chorionic gonadotropin in the cytoplasm of lymphoma cells has not been reported. This patient evidences that...

  15. 77 FR 55481 - Withdrawal of Approval of New Animal Drug Applications; Chorionic Gonadotropin; Naloxone...

    Science.gov (United States)

    2012-09-10

    ...; Chorionic Gonadotropin; Naloxone; Oxymorphone; Oxytocin AGENCY: Food and Drug Administration, HHS. ACTION... Pharmaceuticals hydrochloride) Inc., 100 Painters Injection. Dr., Chadds Ford, PA 19317. 046-822 VETOCIN...

  16. CHROMATOGRAPHIC SEPERATION AND PURIFICATION OF HUMAN CHORIONIC GONADOTROPIN

    Institute of Scientific and Technical Information of China (English)

    WangHaifeng; XuYutai; 等

    1995-01-01

    Separation and purification of human chorionic gonadotropion(HCG) in the urine sample of early pregnant women by D3520 resin adsorption chromatography is reported.The crude product obtained by DEAE-Cellulose 23 and DEAE-Sephadex A50 column chromatography showed a high activity of HCG. Further purification of the sample by gel filtration chromatography on a Sephadex G75 column gives a final preparation of 6000-6500 IU/mg.The preparation meets the requirements of the pyrogn test in Chinese Law of Pharmacopeia.

  17. [Demonstration and evolution of chorionic gonadotropin in ewes].

    Science.gov (United States)

    Lacroix, M C; Martal, J

    1979-02-26

    Occurrence of ovine Chorionic Gonadotropin (oCG) is demonstrated in placenta and amniotic fluid with the use of a radioreceptor assay (corpus luteum membranes) in ewes. Identification of oCG is possible as early as 15th day of pregnancy. It should be secreted at a constant rate, and its maximum concentration is recorded on the 130th day. This hormonal factor might be one of the major components accountable for high progesteronemia observed during the 100 last days of pregnancy. PMID:110484

  18. Chorionic plate arterial function is altered in maternal obesity

    OpenAIRE

    Hayward, C.E.; Higgins, L.; Cowley, E.J.; Greenwood, S. l.; Mills, T.A.; Sibley, C. P.; Wareing, M.

    2013-01-01

    Objectives To characterise Chorionic Plate Artery (CPA) function in maternal obesity, and investigate whether leptin exposure reproduces the obese CPA phenotype in normal-BMI women. Study design CPA responses to the thromboxane-A2 mimetic U46619 (pre/post leptin incubation), to the nitric oxide donor sodium nitroprusside (SNP) and the occurrence of tone oscillations (pre/post leptin incubation) were assessed in 46 term placentas from women of normal (18.5–24.9) or obese (>30) Body Mass Index ...

  19. Fertility Rates of Ewes Treated with Medroxyprogesterone and Injected with Equine Chorionic Gonadotropin plus Human Chorionic Gonadotropin in Anoestrous Season

    Directory of Open Access Journals (Sweden)

    I. W. Santos

    2010-01-01

    Full Text Available The aim of the present paper was to investigate the efficiency of the equine chorionic gonadotropin (eCG plus human chorionic gonadotropin (hCG associated with medroxyprogesterone acetate (MAP to estrous ewes synchronization. Ninety Texel ewes were investigated during seasonal anoestrous. The ewes received intravaginal sponges containing MAP (60 mg for nine days. At the time of sponges' withdrawal, the ewes were divided into three groups (G: (1 receiving 2 mL of saline i.m. (n=30, (2 receiving eCG 400 IU i.m. (n=30, and (3 receiving eCG 400 IU plus hCG 200 IU i.m. (n=30. Twelve h after sponges' removal, teaser rams were used to estrus check and remained with the ewes for 96 h. The artificial insemination was made with fresh semen 10 h after estrus detection. The effect of the treatment was not significant for the estrous rates among the groups: 73%, 90%, and 86%, respectively. The main effect was observed in the pregnancy and lambing rates among the groups: 70%, 86%, 56%, and 80%, 120%, 56%, respectively. Based on these results from our study, the use of the MAP—eCG is the best choice to improve the fertility rate on ewes.

  20. Luteinizing hormone and human chorionic gonadotropin: origins of difference.

    Science.gov (United States)

    Choi, Janet; Smitz, Johan

    2014-03-01

    Luteinizing hormone (LH) and human chorionic gonadotropin (hCG) are widely recognized for their roles in ovulation and the support of early pregnancy. Aside from the timing of expression, however, the differences between LH and hCG have largely been overlooked in the clinical realm because of their similar molecular structures and shared receptor. With technologic advancements, including the development of highly purified and recombinant gonadotropins, researchers now appreciate that these hormones are not as interchangeable as once believed. Although they bind to a common receptor, emerging evidence suggests that LH and hCG have disparate effects on downstream signaling cascades. Increased understanding of the inherent differences between LH and hCG will foster more effective diagnostic and prognostic assays for use in a variety of clinical contexts and support the individualization of treatment strategies for conditions such as infertility.

  1. Chorionic gonadotropin and uterine dialogue in the primate

    Directory of Open Access Journals (Sweden)

    Strakova Zuzana

    2004-07-01

    Full Text Available Abstract Implantation is a complex spatio-temporal interaction between the growing embryo and the mother, where both players need to be highly synchronized to be able to establish an effective communication to ensure a successful pregnancy. Using our in vivo baboon model we have shown that Chorionic Gonadotropin (CG, as the major trophoblast derived signal, not only rescues the corpus luteum but also modulates the uterine environment in preparation for implantation. This response is characterized by an alteration in both the morphological and biochemical activity in the three major cell types: luminal and glandular epithelium and stromal fibroblasts. Furthermore, CG and factors from the ovary have a synergistic effect on the receptive endometrium. Novel local effects of CG which influence the immune system to permit the survival of the fetal allograft and prevent endometrial cell death are also discussed in this review. An alternate extracellular signal-regulated kinase (ERK activation pathway observed in epithelial endometrial cells and the possibility of differential expression of the CG/LH-R isoforms during gestation, open many questions regarding the mechanism of action of CG and its signal transduction pathway within the primate endometrium.

  2. Effect of Mifepristone on the Telomerase Activity in Chorion and Decidua during Early Pregnancy

    Institute of Scientific and Technical Information of China (English)

    Ge-qing XIA; Ya-li XIONG; Yong-hong SUN

    2004-01-01

    Objective To investigate telomerase activity in chorion and decidua from abortion induced by mifepristone incorporated with misoprostol at early pregnancy Methods TRAP-SYBR Green assay was used to detect the expression of telomerase. Forty specimen were obtained from medicinal abortion (experiment group) and forty were from normal induced abortion (control group).Results Positive expression, of chorion telomerase was significantly different between the experimental group (28%, 11/40) and the control group (73%, 29/40) (P<0. 05).While in decidua, the positive rate was 28% (11/40) in the experimental group and 20% (9/40) in the control group, there was no significant difference (P>0. 05).Conclusion It is suggested that miferistone may significantly decrease the telomerase activity in chorion but not in decidua.

  3. Development of ovine chorionic somatomammotropin hormone-deficient pregnancies.

    Science.gov (United States)

    Baker, Callie M; Goetzmann, Lindsey N; Cantlon, Jeremy D; Jeckel, Kimberly M; Winger, Quinton A; Anthony, Russell V

    2016-05-01

    Intrauterine growth restriction (IUGR) is a leading cause of neonatal mortality and morbidity. Chorionic somatomammotropin hormone (CSH), a placenta-specific secretory product found at high concentrations in maternal and fetal circulation throughout gestation, is significantly reduced in human and sheep IUGR pregnancies. The objective of this study was to knock down ovine CSH (oCSH) expression in vivo using lentiviral-mediated short-hairpin RNA to test the hypothesis that oCSH deficiency would result in IUGR of near-term fetal lambs. Three different lentiviral oCSH-targeting constructs were used and compared with pregnancies (n = 8) generated with a scrambled control (SC) lentiviral construct. Pregnancies were harvested at 135 days of gestation. The most effective targeting sequence, "target 6" (tg6; n = 8), yielded pregnancies with significant reductions (P ≤ 0.05) in oCSH mRNA (50%) and protein (38%) concentrations, as well as significant reductions (P ≤ 0.05) in placental (52%) and fetal (32%) weights compared with the SC pregnancies. Fetal liver weights were reduced 41% (P ≤ 0.05), yet fetal liver insulin-like growth factor-I (oIGF1) and -II mRNA concentrations were reduced (P ≤ 0.05) 82 and 71%, respectively, and umbilical artery oIGF1 concentrations were reduced 62% (P ≤ 0.05) in tg6 pregnancies. Additionally, fetal liver oIGF-binding protein (oIGFBP) 2 and oIGFBP3 mRNA concentrations were reduced (P ≤ 0.05), whereas fetal liver oIGFBP1 mRNA concentration was not impacted nor was maternal liver oIGF and oIGFBP mRNA concentrations or uterine artery oIGF1 concentrations (P ≥ 0.10). Based on our results, it appears that oCSH deficiency does result in IUGR, by impacting placental development as well as fetal liver development and function. PMID:26887431

  4. The immunohistochemical features of chorion and endometrium in women with early pregnancy losses

    Directory of Open Access Journals (Sweden)

    Trohimovych O.V.

    2015-03-01

    Full Text Available Background. According to present views proliferation and apoptosis plays an important role in the mechanisms of placenta formation, particularly in process of vasculogenesis that begins on the third week of pregnancy. Objective: To investigate the immunohistochemical features of chorion and endometrium in women with early pregnancy losses. Methods. 98 samples of abortive and surgical material in pregnant women in terms of 4-10 weeks of pregnancy were analyzed. 48 samples were obtained from women with missed abortion (1st group, 20 samples from women with spontaneous abortion (2nd group; 20 samples from women with tubal pregnancy (3rd group; 10 samples from pregnant women with normal singleton pregnancy, after medical abortion on their own wish (control group. Histological, histochemical and immunohistochemical methods were used. Monoclonal antibodies (MAbs to Ki-67 and bcl-2 were applied. Results. Pathological picture of spontaneous abortion is characterized by the dominance of violation of blood circulation, presence of significant foci of hemorrhage occurring parallel with a pronounced disruption of apoptosis and proliferation in chorionic villi and decidual tissue, leading to the detachment of placenta. Conclusion. In 75% of cases of tubal pregnancy ectopic cytotrophoblast invasion is caused by prominent changes in the structure of the fallopian tube. First of all due to the inflammatory process and a sharp reduction of proliferation in the basal chorion and parietal deciduitis in 65% of cases. Citation: Trohimovych OV. [The immunohistochemical features of chorion and endometrium in women with early pregnancy losses]. Morphologia. 2015;9(1:58-64. Russian.

  5. Usefulness of chorionic villus sampling for prenatal diagnosis of thalassaemia: a clinical study in eastern India

    Directory of Open Access Journals (Sweden)

    Sujoy Dasgupta

    2015-06-01

    Conclusions: Thalassaemia is a prevalent condition in Eastern India. Chorionic villus sampling is an effective and safe method for early diagnosis of fetal thalassaemia that helps to prevent birth of thalassaemic babies. [Int J Reprod Contracept Obstet Gynecol 2015; 4(3.000: 790-794

  6. Linear regression of postevacuation serum human chorionic gonadotropin concentrations predicts postmolar gestational trophoblastic neoplasia

    NARCIS (Netherlands)

    Lybol, C.; Sweep, F.C.; Ottevanger, P.B.; Massuger, L.F.A.G.; Thomas, C.M.G.

    2013-01-01

    OBJECTIVE: Currently, human chorionic gonadotropin (hCG) follow-up after evacuation of hydatidiform moles is essential to identify patients requiring chemotherapeutic treatment for gestational trophoblastic neoplasia (GTN). We propose a model based on linear regression of postevacuation serum hCG co

  7. Egg morphology and chorionic ultrastructure of key stored product insect pests of the United States

    Science.gov (United States)

    Eggs of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae) were imaged with scanning electron microscopy to explore how respiratory openings on the chorion surface may be related to the efficacy of fumigants. Each P. interpunctella eg...

  8. Primary pulmonary choriocarcinoma after human chorionic gonadotropin normalization following hydatidiform mole

    DEFF Research Database (Denmark)

    Maestá, Izildinha; Leite, Fábio Vicente; Michelin, Odair Carlito;

    2010-01-01

    BACKGROUND: Primary pulmonary choriocarcinoma (PPC) is rare and frequently leads to death. CASES: Two young patients presented with previous molar pregnancy and spontaneous serum human chorionic gonadotropin (hCG) normalization. Patient 1 was referred to our center after partial response to chemo...

  9. A review of luteinising hormone and human chorionic gonadotropin when used in assisted reproductive technology

    DEFF Research Database (Denmark)

    Ezcurra, Diego; Humaidan, Peter

    2014-01-01

    of the hormones, follicle-stimulating hormone (FSH), luteinising hormone (LH) and human chorionic gonadotropin (hCG), but also other biologically active contaminants, such as growth factors, binding proteins and prions. The actual amount of molecular LH in hMG preparations varies considerably due...

  10. Influence of chorionicity on perinatal outcome in a large cohort of Danish twin pregnancies

    DEFF Research Database (Denmark)

    Oldenburg, Anna; Rode, Line; Bødker, Birgit;

    2012-01-01

    Objective To assess outcome in twin pregnancies according to chorionicity. Methods A cohort was retrieved from local ultrasound databases at 14 obstetric departments in Denmark, comprising all twin pregnancies with two live fetuses scanned between weeks 11 to 14 in the period 1 January 2004 to 31...

  11. Pre-eclampsia (PE) and Chorionicity in Women with Twin Gestations

    Science.gov (United States)

    Singh, Anupama; Singh, Arati; Surapaneni, Tarakeswari; Nirmalan, Praveen Kumar

    2014-01-01

    Background: Pre-Eclampsia (PE) affects 6-31% of pregnant women with multiple gestations. There are conflicting reports on the association of PE with Chorionicity and zygosity; however, there is a lack of information on this potential association in a population of pregnant Asian Indian women. Aim: To determine as to whether chorionicity and zygosity were associated with PE in a population of Asian Indian women with twin gestations. Settings and Design: A retrospective observational study was done at a single tertiary care centre in Southern India. Material and Methods: The study included pregnant women with twin gestations, who was delivered at the study institute in 2012. Hypertension in pregnancy was categorized, based on the criteria of the International Society for the Study of Hypertension in Pregnancy. Chorionicity was determined by using ultrasonography and zygosity was determined, based on clinical criteria. Point estimates and the 95% Confidence Intervals (CI) around point estimates of PE and associations of chorionicity and zygosity with PE were determined by using bivariate analysis, logistic regression models and area under Receiver Operator Characteristic (ROC) curves. Results: This study included 208 women with twin gestations. The incidence of PE in dichorionic twin gestations was 13.17% (n=22, 95% CI: 8.66, 18.96), it was 4.87% (n=2, 95% CI: 0.83, 15.19) in monochorionic twin gestations, it was 16.36% (n=9, 95% CI: 8.29, 27.91) in dizygous twin gestations and it was 4.88% (n=2, 95% CI: 0.83, 15.19) in monozygous twin gestations. Neither chorionicity (adjusted OR: 2.59, 95% CI: 0.55, 12.19) nor zygosity (adjusted OR 2.72, 95% CI: 0.49, 15.13) were associated with PE In a multivariate logistic regression model. Conclusion: Although it was not statistically significant, the clinical incidence of PE was higher in dichorionic and dizygous twin gestations. PMID:24596736

  12. Characterization of N-linked oligosaccharides in chorion peroxidase of Aedes aegypti mosquito.

    Science.gov (United States)

    Li, Junsuo S; Li, Jianyong

    2005-09-01

    A peroxidase is present in the chorion of Aedes aegypti eggs and catalyzes chorion protein cross-linking during chorion hardening, which is critical for egg survival in the environment. The unique chorion peroxidase (CPO) is a glycoprotein. This study deals with the N-glycosylation site, structures, and profile of CPO-associated oligosaccharides using mass spectrometric techniques and enzymatic digestion. CPO was isolated from chorion by solubilization and several chromatographic methods. Mono-saccharide composition was analyzed by HPLC with fluorescent detection. Our data revealed that carbohydrate (D-mannose, N-acetyl D-glucosamine, D-arabinose, N-acetyl D-galactosamine, and L-fucose) accounted for 2.24% of the CPO molecular weight. A single N-glycosylation site (Asn328-Cys- Thr) was identified by tryptic peptide mapping and de novo sequencing of native and PNGase A-deglycosylated CPO using matrix-assisted laser/desorption/ionization time-of-flight mass spectrometry (MALDI/TOF/MS) and liquid chromatography/tandem mass spectrometry (LC/MS/MS). The Asn328 was proven to be a major fully glycosylated site. Potential tryptic glycopeptides and profile were first assessed by MALDI/TOF/MS and then by precursor ion scanning during LC/MS/MS. The structures of N-linked oligosaccharides were elucidated from the MS/MS spectra of glycopeptides and exoglycosidase sequencing of PNGase A-released oligosaccharides. These CPO-associated oligosaccharides had dominant Man3GlcNAc2 and Man3 (Fuc) GlcNAc2 and high mannose-type structures (Man(4-8)GlcNAc2). The truncated structures, Man2GlcNAc2 and Man2 (Fuc) GlcNAc2, were also identified. Comparison of CPO activity and Stokes radius between native and deglycosylated CPO suggests that the N-linked oligosaccharides influence the enzyme activity by stabilizing its folded state.

  13. Retrospective study of trisomy 18 in chorionic villi with fluorescent in situ hybridization on archival direct preparations

    OpenAIRE

    Van Opstal, Diane; Berg, Cardi; Jahoda, M.; Brandenburg, Helen; Los, F.J.; in't Veld, Peter

    1995-01-01

    textabstractTrisomy 18 in direct chorionic villus preparations needs further investigation since the chromosome abnormality may be confined to the placenta and may not represent the actual fetal karyotype. We performed, retrospectively, fluorescent in situ hybridization (FISH) with the chromosome 18 centromere probe (L1.84) on interphase nuclei of destained slides of all cases of full trisomy 18 (n=22) and mosaic trisomy 18 (n=8) detected among 7600 first-trimester chorionic villus samples du...

  14. Generation of iPSC lines from primary human chorionic villi cells

    Directory of Open Access Journals (Sweden)

    Björn Lichtner

    2015-11-01

    Full Text Available Primary human chorionic villi (CV cells were used to generate the iPSC line by retroviral transduction of the four Yamanaka-factors OCT4, SOX2, KLF4 and c-MYC. Pluripotency was confirmed both in vivo and in vitro. The transcriptomes of the CV-derived iPSC lines and the human embryonic stem cell lines—H1 and H9 have a Pearson correlation of 0.929 and 0.943 respectively.

  15. Expression of human chorionic gonadotropin beta in gastric carcinoma: A retrospective immunohistochemical study

    OpenAIRE

    Murhekar Kanchan; Anuratha Jayaram; Majhi Urmila; Rajkumar Thangarajan

    2009-01-01

    Context: Beta Human Chorionic Gonadotropin (βHCG), a marker of the trophoblastic neoplasm, is also secreted by non-trophoblastic neoplasms including gastric carcinomas. Its role in disease progression remains unclear. Aim: To investigate the incidence of βHCG positivity in gastric carcinomas and correlate its presence with the biological behavior of the tumor. Setting and Design: A hospital-based, immunohistochemical study. Materials and Methods: One hundred and fifty formali...

  16. Ovarian reaction and estrus manifestation in delayed puberty gilts after treatment with equine chorionic gonadotropin

    OpenAIRE

    Stančić Ivan B; Bošnjak Darko V; Radović Ivan B; Stančić Blagoje L.; Harvey Roger B; Anderson Robin C

    2012-01-01

    Abstract Background Prolonged pre-insemination anestrus (i.e. delayed puberty) is a major contributing factor for culling up to 30% of the replacement gilts at large breeding farm units in Vojvodina. It is imperative to determine if these gilts are acyclic (prepubertal) or cyclic, but just fail to exhibit behavioural estrus. Recent investigations demonstrate that treatment with equine chorionic gonadotropin (eCG) can increase the diestrous phase duration in sexually mature gilts. Based on the...

  17. Amnion and Chorion Membranes: Potential Stem Cell Reservoir with Wide Applications in Periodontics

    OpenAIRE

    Akanksha Gupta; Suresh D. Kedige; Kanu Jain

    2015-01-01

    The periodontal therapy usually aims at elimination of disease causing bacteria and resolution of inflammation. It involves either resective or regenerative surgery to resolve the inflammation associated defects. Over the years, several methods have been used for achievement of periodontal regeneration. One of the oldest biomaterials used for scaffolds is the fetal membrane. The amniotic membranes of developing embryo, that is, amnion (innermost lining) and chorion (a layer next to it), have ...

  18. Equine Chorionic Gonadotropin and Human Chorionic Gonadotropin Stimulation Increase the Number of Luteinized Follicles and the Progesterone Level Compared with Cabergoline Stimulation in Anoestrus Bitches.

    Science.gov (United States)

    Jurczak, A; Domosławska, A; Bukowska, B; Janowski, T

    2016-08-01

    In this study, ovarian morphologies and blood progesterone concentrations following oestrous induction in bitches were examined. Fifty-three clinically healthy anoestrus bitches received cabergoline at a daily dose of 5 μg/kg of body weight per os for 21 days (group I) or subcutaneous equine chorionic gonadotropin at a dose of 20 IU/kg of body weight for five consecutive days with an additional 500 IU s.c. per bitch of human chorionic gonadotropin on the last day of treatment (group II). Twenty bitches that spontaneously displayed oestrous signs were left untreated and served as controls (group III). The induced oestrous rates and ovulation rates in groups I and II were 60.0% vs 64.3% and 86.7% vs 83.3%, respectively. Morphological assessments of the ovarian structures after ovariohysterectomy revealed an increase in the number of luteinized follicles and cysts in group II compared with the two other groups (p similar in all groups. In accordance with the above-mentioned alteration, the progesterone concentration in the gonadotropin group (II) was increased (p similar and typical of normally cycling bitches. In conclusion, gonadotropin treatment is associated with an increased progesterone level during the periovulatory period that probably originates from luteinized follicles, whereas cabergoline treatment induces cycles with both physiological progesterone concentrations and ovarian morphologies. PMID:27321641

  19. An unexpected reason for elevated human chorionic gonadotropin in a young woman. Cervical squamous carcinoma.

    Science.gov (United States)

    Mustafa, Aynur; Bozdag, Zehra; Tepe, Neslihan B; Ozcan, Husiyen C

    2016-08-01

    Human chorionic gonadotropin has been used for decades, in addition to specific investigations, to detect pregnancy, trophoblastic tumors, as well as congenital defects. Rarely, it can be elevated in  non-trophoblastic tumors such as squamous cell cancers and germ cell tumors. A 33-year-old Asian Syrian female had irregular menses accompanied with feelings of heaviness in the vagina. In addition to routine investigations, we measured the serum beta human chorionic gonadotropin (ß-HCG) level (based on the patient's complaint of amenorrhea), which was 50.05 ml UI/ml. Cervical biopsy revealed a non-keratinized large cell squamous carcinoma. After excluding other causes, ß-hCG elevation was explained by the ectopic secretion of cancer cells line. Cervical biopsy was suggestive of large cell non-keratinizing squamous cell carcinoma and positive for human chorionic gonadotropin on immunohistochemistry. As a result, we manage the possibility of ectopic secretion of ß-HCG from non- trophoblastic disease. PMID:27464870

  20. An unexpected reason for elevated human chorionic gonadotropin in a young woman Cervical squamous carcinoma

    Science.gov (United States)

    Mustafa, Aynur; Bozdağ, Zehra; Tepe, Neslihan B.; Ozcan, Huseyin C.

    2016-01-01

    Human chorionic gonadotropin has been used for decades, in addition to specific investigations, to detect pregnancy, trophoblastic tumors, as well as congenital defects. Rarely, it can be elevated in non-trophoblastic tumors such as squamous cell cancers and germ cell tumors. A 33-year-old Asian Syrian female had irregular menses accompanied with feelings of heaviness in the vagina. In addition to routine investigations, we measured the serum beta human chorionic gonadotropin (ß-HCG) level (based on the patient’s complaint of amenorrhea), which was 50.05 ml UI/ml. Cervical biopsy revealed a non-keratinized large cell squamous carcinoma. After excluding other causes, ß-hCG elevation was explained by the ectopic secretion of cancer cells line. Cervical biopsy was suggestive of large cell non-keratinizing squamous cell carcinoma and positive for human chorionic gonadotropin on immunohistochemistry. As a result, we manage the possibility of ectopic secretion of ß-HCG from non- trophoblastic disease. PMID:27464870

  1. Reduced expression of 15-hydroxy prostaglandin dehydrogenase in chorion during labor is associated with decreased PRB and increased PRA and GR expression.

    Science.gov (United States)

    Li, Yuan; He, Ping; Sun, Qianqian; Liu, Jie; Gao, Lu; You, Xingji; Gu, Hang; Ni, Xin

    2013-05-01

    The chorion laeve controls the levels of active prostaglandins within the uterus by NAD-dependent 15-hydroxy prostaglandin dehydrogenase (PGDH). The expression of PGDH in chorion is modulated by glucocorticoids and progesterone. In this study, we investigated glucocorticoid receptor (GR) and progesterone receptor A and B (PRA and PRB) in the regulation of PGDH expression in chorion, and we determined whether reduced PGDH expression in chorion during labor is associated with the changes in GR and PR expression by real-time RT-PCR and Western blot analysis. Dexamethasone (DEX) inhibited PGDH expression whereas progesterone stimulated PGDH expression in chorionic trophoblasts. DEX suppressed PGDH expression in GR overexpression and PR knockdown cells. The inhibitory effect of DEX did not occur in GR knockdown cells. Progesterone inhibited PGDH in GR overexpression and PR knockdown cells and it stimulated PGDH in PRB overexpression cells whereas it suppressed PGDH in PRA overexpression cells. Knockdown of c-Jun resulted in a loss of progesterone- and DEX-induced effects. PGDH was down-regulated in chorion tissues during labor. PRB was decreased whereas PRA and GR were increased in chorion during labor. Glucocorticoids inhibit PGDH expression via GR in chorionic trophoblasts. Progesterone enhances PGDH expression through PRB, whereas it inhibits PGDH expression via GR and PRA. Decreased PGDH expression is associated with increased GR and PRA, although decreased PRB, in chorion during labor.

  2. Effects and mechanisms of action of sildenafil citrate in human chorionic arteries.

    LENUS (Irish Health Repository)

    Maharaj, Chrisen H

    2009-01-01

    OBJECTIVES: Sildenafil citrate, a specific phosphodiesterase-5 inhibitor, is increasingly used for pulmonary hypertension in pregnancy. Sildenafil is also emerging as a potential candidate for the treatment of intra-uterine growth retardation and for premature labor. Its effects in the feto-placental circulation are not known. Our objectives were to determine whether phosphodiesterase-5 is present in the human feto-placental circulation, and to characterize the effects and mechanisms of action of sildenafil citrate in this circulation. STUDY DESIGN: Ex vivo human chorionic plate arterial rings were used in all experiments. The presence of phosphodiesterase-5 in the feto-placental circulation was determined by western blotting and immunohistochemical staining. In a subsequent series of pharmacologic studies, the effects of sildenafil citrate in pre-constricted chorionic plate arterial rings were determined. Additional studies examined the role of cGMP and nitric oxide in mediating the effects of sildenafil. RESULTS: Phosphodiesterase-5 mRNA and protein was demonstrated in human chorionic plate arteries. Immunohistochemistry demonstrated phosphodiesterase-5 within the arterial muscle layer. Sildenafil citrate produced dose dependent vasodilatation at concentrations at and greater than 10 nM. Both the direct cGMP inhibitor methylene blue and the cGMP-dependent protein kinase inhibitor Rp-8-Br-PET-cGMPS significantly attenuated the vasodilation produced by sildenafil citrate. Inhibition of NO production with L-NAME did not attenuate the vasodilator effects of sildenafil. In contrast, sildenafil citrate significantly enhanced the vasodilation produced by the NO donor sodium nitroprusside. CONCLUSION: Phosphodiesterase-5 is present in the feto-placental circulation. Sildenafil citrate vasodilates the feto-placental circulation via a cGMP dependent mechanism involving increased responsiveness to NO.

  3. Small gene family encoding an eggshell (chorion) protein of the human parasite Schistosoma mansoni

    Energy Technology Data Exchange (ETDEWEB)

    Bobek, L.A.; Rekosh, D.M.; Lo Verde, P.T.

    1988-08-01

    The authors isolated six independent genomic clones encoding schistosome chorion or eggshell proteins from a Schistosoma mansoni genomic library. A linkage map of five of the clones spanning 35 kilobase pairs (kbp) of the S. mansoni genome was constructed. The region contained two eggshell protein genes closely linked, separated by 7.5 kbp of intergenic DNA. The two genes of the cluster were arranged in the same orientation, that is, they were transcribed from the same strand. The sixth clone probably represents a third copy of the eggshell gene that is not contained within the 35-kbp region. The 5- end of the mRNA transcribed from these genes was defined by primer extension directly off the RNA. The ATCAT cap site sequence was homologous to a silkmoth chorion PuTCATT cap site sequence, where Pu indicates any purine. DNA sequence analysis showed that there were no introns in these genes. The DNA sequences of the three genes were very homologous to each other and to a cDNA clone, pSMf61-46, differing only in three or four nucleotices. A multiple TATA box was located at positions -23 to -31, and a CAAAT sequence was located at -52 upstream of the eggshell transcription unit. Comparison of sequences in regions further upstream with silkmoth and Drosophila sequences revealed very short elements that were shared. One such element, TCACGT, recently shown to be an essential cis-regulatory element for silkmoth chorion gene promoter function, was found at a similar position in all three organisms.

  4. [Exclusion of Sandhoff disease (Tay-Sachs 0 variant) by chorion biopsy].

    Science.gov (United States)

    Veszprémi, B; Baranyai, Z; Klujber, L; Arany, A

    1992-04-01

    Transcervical chorionic villus sampling with ultrasound guidance at the 11-th week of pregnancy was made at a woman with the history of one lethal case of Sandhoff disease. The total hexosaminidase and the hexosaminidase A were determined. At the 16-th week amniocentesis was performed and the characteristic enzymes were determined from the amniotic cell culture. The results of the examinations made possible to advise the patient to carry out the pregnancy. The examinations after delivery confirmed the newborn to be a carrier. PMID:1522989

  5. Quantitation of human chorionic gonadotropin with the Abbott IMx immunochemistry analyzer.

    Science.gov (United States)

    van Oers, R J; Leerkes, B; Bertschi, I

    1990-01-01

    The analytical performance of the Abbott IMx automated system was studied and initial results from the human chorionic gonadotropin assay are presented: Within run and between day precision was good, the majority of all CVs lying between 3 and 5%; Patient results showed excellent comparability with our routine method; No significant specimen-related carry-over was detected; The automated IMx dilution protocol was found to be superior to the manual procedure; The usefulness of the instrument in routine use and as a "Stat" analyzer was very good. PMID:2179460

  6. [Mechanism of regulation of synthesis and secretion of human chorionic gonadotropin (hCG) during pregnancy].

    Science.gov (United States)

    Barrera, David; Chirinos, Mayel; García-Becerra, Rocío

    2008-01-01

    Human chorionic gonadotropin (hCG) is an essential hormone for development and sustaining of gestation. Adequate hCG production is fundamental for pregnancy success since abnormal hCG serum concentrations have been correlated with pregnancy anomalies such as recurrent abortions and preeclampsia. Regulation of hCG production involves diverse molecules associated with different signaling pathways, which have complicated the establishment of the mechanisms involved in its production. The present study provides a critical review of the most relevant findings related to hCG production and functions during pregnancy, in order to help to understand some related pathologies and to treat them more adequately.

  7. The birds and the beans: a low-fidelity simulator for chorionic villus sampling skill acquisition.

    Science.gov (United States)

    Wax, Joseph R; Cartin, Angelina; Pinette, Michael G

    2012-08-01

    Because no simulation models are described for chorionic villus sampling (CVS), we sought to design and construct a CVS training simulator. Using materials available from our labor floor and local supermarket, we built and demonstrated a practical model for learning transabdominal and transcervical CVS. The simulator can be used to teach single- or dual-operator transabdominal CVS and traditional transcervical CVS. Aspirated "villi" immediately inform the teacher and learner of successful procedures. No image degradation or sonographically visible tracks resulted from use, permitting more than one trainee to benefit from a model. This model for transabdominal and transcervical CVS provides realistic imaging, tactile sensations, and immediate feedback.

  8. 1ST-TRIMESTER MATERNAL SERUM HUMAN CHORIONIC-GONADOTROPIN AS A MARKER FOR FETAL CHROMOSOMAL DISORDERS

    NARCIS (Netherlands)

    VANLITH, JMM

    1992-01-01

    The Dutch Working Party on Prenatal Diagnosis has initiated a study on the possibilities of first-trimester screening for fetal chromosomal disorders. We report on maternal serum human chorionic gonadotrophin (MS-hCG) measurements in 1348 pregnancies with a chromosomally normal fetus and 53 pregnanc

  9. Prediction of miscarriage and stillbirth at 11-13 weeks and the contribution of chorionic villus sampling

    NARCIS (Netherlands)

    Akolekar, Ranjit; Bower, Sarah; Flack, Nicola; Bilardo, Caterina M.; Nicolaides, Kypros H.

    2011-01-01

    Objectives To derive models for estimating risk of miscarriage and stillbirth from maternal characteristics and findings of first-trimester screening for aneuploidies and to define the procedure-related risk of chorionic villus sampling (CVS) after adjusting for these factors. Method We examined 33

  10. Retrospective study of trisomy 18 in chorionic villi with fluorescent in situ hybridization on archival direct preparations

    NARCIS (Netherlands)

    A.R.M. van Opstal (Diane); C.D.F. van den Berg (Cardi); M.G. Jahoda (M.); H. Brandenburg (Helen); F.J. Los; P.A. In't Veld (Peter)

    1995-01-01

    textabstractTrisomy 18 in direct chorionic villus preparations needs further investigation since the chromosome abnormality may be confined to the placenta and may not represent the actual fetal karyotype. We performed, retrospectively, fluorescent in situ hybridization (FISH) with the chromosome 18

  11. beta-glucuronidase deficiency as cause of recurrent hydrops fetalis : The first early prenatal diagnosis by chorionic villus sampling

    NARCIS (Netherlands)

    Van Eyndhoven, HWF; Ter Brugge, HG; Van Essen, AJ; Kleijer, WJ

    1998-01-01

    We describe a patient with recurrent non-immune hydrops fetalis diagnosed as mucopolysaccharidosis type VII. This rare autosomal recessive disorder is caused by a beta-glucuronidase deficiency. Chorionic villus sampling was performed in the 11 th week of the subsequent pregnancy and beta-glucuronida

  12. Biological variation of free β chorionic gonadotropin and pregnancy-associated plasma protein A in first trimester pregnancies

    DEFF Research Database (Denmark)

    Sennels, Henriette P; Jørgensen, Finn Stener; Sørensen, Steen

    2011-01-01

    Abstract Background: Trisomy 21 risk estimation in first trimester pregnancies can be performed by a combined test based on ultrasound measurement of fetal nuchal translucency thickness and maternal plasma concentrations of free β human chorionic gonadotropin (hCGβ) and pregnancy-associated plasma...

  13. Biological variation of free β chorionic gonadotropin and pregnancy-associated plasma protein A in first trimester pregnancies

    DEFF Research Database (Denmark)

    Sennels, Henriette P; Jørgensen, Finn Stener; Sørensen, Steen

    2011-01-01

    Abstract Background: Trisomy 21 risk estimation in first trimester pregnancies can be performed by a combined test based on ultrasound measurement of fetal nuchal translucency thickness and maternal plasma concentrations of free ß human chorionic gonadotropin (hCGß) and pregnancy-associated plasma...

  14. Fetal ductus venosus flow velocity waveforms and maternal serum AFP before and after first-trimester transabdominal chorionic villus sampling

    NARCIS (Netherlands)

    C.A. Brezinka (Christoph); A.M. Hagenaars (A.); J.W. Wladimiroff (Juriy); F.J. Los

    1995-01-01

    textabstractDoppler flow velocity waveform recording in the fetal ductus venosus and umbilical artery as well as maternal blood sampling for serum alpha-fetoprotein (MSAFP) was performed before and after transabdominal chorion villus sampling (TACVS) in 36 women of advanced maternal age (≥ 36 years)

  15. Evidence for, and Associated Risks with, the Human Chorionic Gonadotropin Supplemented Diet.

    Science.gov (United States)

    Butler, Stephen A; Cole, Laurence A

    2016-11-01

    Trend diets can be commonplace amongst those who are trying to lose weight but in most cases there is some shred of evidence to suggest they might be of some benefit. Seldom is there a diet which is such a fad that it is not only completely unfounded but also potential harmful. The human chorionic gonadotropin or "hCG diet" is such a diet, which after half a century still has no evidence to support its efficacy; in fact all scientific publications subsequent to the original article counter these claims. In this short communication, we review the literature and present data on exactly what some of the hCG diet preparations actually contain and highlight that, based on current data, these may do more harm than good. It is worrying that more consideration is not given to the possible danger of administration of hCG preparations to individuals without an evidence-based rational. PMID:27010890

  16. Mechanism of Trypanosoma cruzi Placenta Invasion and Infection: The Use of Human Chorionic Villi Explants

    Directory of Open Access Journals (Sweden)

    Ricardo E. Fretes

    2012-01-01

    Full Text Available Congenital Chagas disease, a neglected tropical disease, endemic in Latin America, is associated with premature labor and miscarriage. During vertical transmission the parasite Trypanosoma cruzi (T. cruzi crosses the placental barrier. However, the exact mechanism of the placental infection remains unclear. We review the congenital transmission of T. cruzi, particularly the role of possible local placental factors that contribute to the vertical transmission of the parasite. Additionally, we analyze the different methods available for studying the congenital transmission of the parasite. In that context, the ex vivo infection with T. cruzi trypomastigotes of human placental chorionic villi constitutes an excellent tool for studying parasite infection strategies as well as possible local antiparasitic mechanisms.

  17. A model for gas and nutrient exchange in the chorionic vasculature system of the mouse placenta

    Science.gov (United States)

    Mirbod, Parisa; Sled, John

    2015-11-01

    The aim of this study is to develop an analytical model for the oxygen and nutrient transport from the umbilical cord to the small villous capillaries. The nutrient and carbon dioxide removal from the fetal cotyledons in the mouse placental system has also been considered. This model describes the mass transfer between the fetal and the maternal red blood cells in the chorionic arterial vasculature system. The model reveals the detail fetal vasculature system and its geometry and the precise mechanisms of mass transfer through the placenta. The dimensions of the villous capillaries, the total length of the villous trees, the total villi surface area, and the total resistance to mass transport in the fetal villous trees has also been defined. This is the first effort to explain the reason why there are at least 7 lobules in the mouse placenta from the fluid dynamics point of view.

  18. Prenatal cytogenetic diagnosis after transabdominal chorionic villus sampling in the first trimester

    DEFF Research Database (Denmark)

    Therkelsen, A J; Jensen, P K; Hertz, Jens Michael;

    1988-01-01

    First trimester prenatal cytogenetic diagnosis was attempted in 350 pregnancies after transabdominal chorionic villus sampling. The cytogenetic investigation was performed using both a short-term method (24 h incubation) and cell culture. Adequate samples were obtained in 99.1 per cent and in all...... these cases the fetal karyotype was established. A chromosome abnormality was found in 2.0 per cent of cases. A discrepancy between the karyotype obtained after 24 h incubation and the karyotype in cell culture was observed in 2.3 per cent. Maternal cell contamination in the cultures was confirmed in 13...... cytogenetic investigation until the problems with karyotype discrepancy and maternal cell contamination have been further clarified....

  19. Choriocarcinoma with negative urinary and serum beta human chorionic gonadotropin (β HCG : A case report

    Directory of Open Access Journals (Sweden)

    Mehra Reeti

    2005-12-01

    Full Text Available This was a rare case where a patient presented clinically as a case of post abortal sepsis and ultrasound showing the picture of an intramural degenerating fibroid. Her serum and urine both were negative for b human chorionic gonadotropin (bHCG. Patient succumbed to choriocarcinoma 1 month later. Failure to detect urinary and serum bHCG lead to maternal mortality due to the choriocarcinoma. The failure to detect, certain degradation products of HCG which may predominate in gestational trophoblastic neoplasia, by many common HCG testing kits lead to the error of diagnosis. Only 3 of the 7 common commercial serum HCG tests appropriately detects nicked HCG and its free bHCG, DPC immulite assay, being the most sensitive method. Though of rare occurrence, this awareness is important for diagnosis and follow-up of gestational trophoblastic neoplasia and could have been life saving in our case.

  20. Mussel adhesive enhances the immobilization of human chorionic gonadotrophin to a solid support.

    Science.gov (United States)

    Burzio, V A; Silva, T; Pardo, J; Burzio, L O

    1996-10-15

    Polystyrene microtiter plates coated with 0.30 microgram/ well of the adhesive polyphenolic protein purified from the mussel Aulacomya ater showed enhanced capacity to immobilize antigens such as human chorionic gonadotrophin (hCG). Uncoated and coated wells were activated with different amounts of hCG (from 2 to 500 ng), blocked with bovine serum albumin, and tested with anti-hCG monoclonal antibodies and antimouse IgG conjugated with peroxidase. The reading at 492 nm of the uncoated wells activated with 500 ng of hCG was similar to that obtained with coated wells but using 5 to 10 ng of antigen. The coating procedure also resulted in better sensitivity to detect low concentration of monoclonal antibodies and better signal-to-noise ratio. The capacity of the mussel coating to immobilize hCG, as well as the immunoreactivity of the attached antigen, remained stable for several months. PMID:8921186

  1. Studies on stabilities of some human chorionic gonadotropin complexes with {beta}-emitting radionuclides

    Energy Technology Data Exchange (ETDEWEB)

    Maiti, Moumita [Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064 (India); Sen, Kamalika [Department of Chemistry, University of Calcutta, 92 APC Road, Kolkata 700009 (India); Sen, Souvik [Malda Town Divisional Railway Hospital, Malda 732102 (India); Lahiri, Susanta, E-mail: susanta.lahiri@saha.ac.i [Chemical Sciences Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064 (India)

    2011-02-15

    Human chorionic gonadotropin (hCG) is a peptide hormone, whose one of the structural subunits is identical to that of thyroid-stimulating hormone (TSH). As a consequence, the receptors of TSH also act as receptor for hCG hormone. Keeping in mind this interesting property of hCG we have studied the complex formation ability of various no-carrier-added {beta}-emitting isotopes of {sup 61}Cu (3.3 h), {sup 62}Zn (9.2 h), {sup 90}Nb (14.60 h) and {sup 99}Mo (66.02 h) with hCG molecule. Stability of the hCG-M (M=metal ions) complexes was investigated by dialysis with respect to triple distilled water and ringer lactate solution, which has the same composition as extracellular fluid.

  2. Nuclear Translocation of Nuclear Factor Kappa B in First Trimester Deciduas and Chorionic Villi in Early Spontaneous Miscarriage Women

    Directory of Open Access Journals (Sweden)

    Chun-fang Yan

    2010-02-01

    Full Text Available The nuclear factor kappa B is widely expressed in the distinct subpopulations of chorionic villi and deciduas of first-trimester pregnancies. We examined the cellular distribution and expression of nuclear factor kappa B in the human first-trimester chorionic villi and deciduas of women with early spontaneous miscarriage and viable pregnancy by confocal laser scanning microscope and immunohistochemistry. There is a greater nuclear translocation of nuclear factor kappa B is restricted to villous stromal cells, decidual stromal cells, glandular epithelial cells and vessel endothelial cells in early spontaneous miscarriage than in viable pregnancies. Collectively these observations suggest that over-activation of nuclear factor kappa B has a relationship with early spontaneous miscarriages.

  3. Human chorionic gonadotropin administration is associated with high pregnancy rates during ovarian stimulation and timed intercourse or intrauterine insemination

    OpenAIRE

    Abdel-Razeq Sonya; Mitwally Mohamed F; Casper Robert F

    2004-01-01

    Abstract Background There are different factors that influence treatment outcome after ovarian stimulation and timed-intercourse or intrauterine insemination (IUI). After patient age, it has been suggested that timing of insemination in relation to ovulation is probably the most important variable affecting the success of treatment. The objective of this study is to study the value of human chorionic gonadotropin (hCG) administration and occurrence of luteinizing hormone (LH) surge in timing ...

  4. Angiogenic properties of dehydrated human amnion/chorion allografts: therapeutic potential for soft tissue repair and regeneration

    OpenAIRE

    Koob, Thomas J; Lim, Jeremy J.; Massee, Michelle; Zabek, Nicole; Rennert, Robert; Gurtner, Geoffrey; Li, William W

    2014-01-01

    Background Chronic wounds are associated with a number of deficiencies in critical wound healing processes, including growth factor signaling and neovascularization. Human-derived placental tissues are rich in regenerative cytokines and have been shown in randomized clinical trials to be effective for healing chronic wounds. In this study, PURION® Processed (MiMedx Group, Marietta, GA) dehydrated human amnion/chorion membrane tissue allografts (dHACM, EpiFix®, MiMedx) were evaluated for prope...

  5. Association between altered placental human chorionic gonadotrophin (hCG) production and the occurrence of cryptorchidism: a retrospective study

    OpenAIRE

    Chedane, Carole; Puissant, Hugues; Weil, Dominique; Rouleau, Stéphanie; Coutant, Régis

    2014-01-01

    Background An increase in cryptorchidism has been reported in many countries. One mechanism could be low fetal testosterone production possibly secondary to altered placental human chorionic gonadotrophin (hCG) release. Our Objective was to compare hCG values from maternal blood between boys with cryptorchidism and normal boys. Methods Total hCG and α-fetoprotein (AFP) values [12–16 weeks of gestation; from the double test for Down syndrome screening) were compared between cases of cryptorchi...

  6. Changes of serum and chorion-villi contents of EGF in early pregnant women undergone artificial abortion

    International Nuclear Information System (INIS)

    Objective: To investigate the changes of serum and chorion-villi contents of EGF in pregnant women undergone artificial abortion with drug (mifepristone) or surgery (curettage). Methods: Serum epidermal growth factor (EGF), E2, progesterone levels changes as well as chorion-villi EGF contents were measured with RIA in 36 pregnant women with drug abortion (before and after mifepristone 25mg bid x 3 days), 30 pregnant women undergone curettage (determined twice, 3 days apart) and 32 controls (serum only). Results: Serum EGF, E2, and progesterone contents in all pregnant women were significantly higher than those in controls (P<0.01). The chorion-villi contents of EGF in patients undergone drug abortion were significantly lower than those in patients undergone curettage (P<0.05). Both serum EGF and progesterone contents dropped after 3 days treatment with mifepristone (vs those in curettage group, P<0.05). Conclusion: Mifepristone might exert the effect of abortion through decrease of EGF levels, which was detrimental to fetus growth. (authors)

  7. Ovarian hyperstimulation syndrome prevention strategies: reducing the human chorionic gonadotropin trigger dose.

    Science.gov (United States)

    Kashyap, Sonya; Parker, Kasey; Cedars, Marcelle I; Rosenwaks, Zev

    2010-11-01

    This article reviews the biological plausibility and evidence for the use of a low triggering dose of human chorionic gonadotropin (hCG) in the prevention of ovarian hyperstimulation syndrome (OHSS). A systematic search of the literature revealed very little published data for or against the use of low-dose hCG in the prevention of OHSS after assisted reproductive technology. We have had success at avoiding OHSS as a result of gentle stimulation and low-dose sliding scale hCG trigger based on estradiol (E₂) levels. We therefore present the biological plausibility for such an approach by reviewing the relationship between OHSS, vascular endothelial growth factor, and hCG; the physiology of hCG; the relationship between risk of OHSS and E₂ at trigger; and the physiology of alternative methods of triggering such as recombinant luteinizing hormone and gonadotropin-releasing hormone agonist. We also present the results of a quasi-experimental before and after study of the sliding scale protocol for hCG trigger dose in in vitro fertilization with or without intracytoplasmic sperm injection cycles.

  8. A Graphene Oxide-Based Fluorescent Method for the Detection of Human Chorionic Gonadotropin

    Directory of Open Access Journals (Sweden)

    Ning Xia

    2016-10-01

    Full Text Available Human chorionic gonadotropin (hCG has been regarded as a biomarker for the diagnosis of pregnancy and some cancers. Because the currently used methods (e.g., disposable Point of Care Testing (POCT device for hCG detection require the use of many less stable antibodies, simple and cost-effective methods for the sensitive and selective detection of hCG have always been desired. In this work, we have developed a graphene oxide (GO-based fluorescent platform for the detection of hCG using a fluorescein isothiocyanate (FITC-labeled hCG-specific binding peptide aptamer (denoted as FITC-PPLRINRHILTR as the probe, which can be manufactured cheaply and consistently. Specifically, FITC-PPLRINRHILTR adsorbed onto the surface of GO via electrostatic interaction showed a poor fluorescence signal. The specific binding of hCG to FITC-PPLRINRHILTR resulted in the release of the peptide from the GO surface. As a result, an enhanced fluorescence signal was observed. The fluorescence intensity was directly proportional to the hCG concentration in the range of 0.05–20 IU/mL. The detection limit was found to be 20 mIU/mL. The amenability of the strategy to hCG analysis in biological fluids was demonstrated by assaying hCG in the urine samples.

  9. Interaction Of Calcium Phosphate Nanoparticles With Human Chorionic Gonadotropin Modifies Secondary And Tertiary Protein Structure

    Directory of Open Access Journals (Sweden)

    Al-Hakeim Hussein K

    2015-12-01

    Full Text Available Calcium phosphate nanoparticles (CaPNP have good biocompatibility and bioactivity inside human body. In this study, the interaction between CaPNP and human chorionic gonadotropin (hCG was analyzed to determine the changes in the protein structure in the presence of CaPNP and the quantity of protein adsorbed on the CaPNP surface. The results showed a significant adsorption of hCG on the CaPNP nanoparticle surface. The optimal fit was achieved using the Sips isotherm equation with a maximum adsorption capacity of 68.23 µg/mg. The thermodynamic parameters, including ∆H° and ∆G°, of the adsorption process are positive, whereas ∆S° is negative. The circular dichroism results of the adsorption of hCG on CaPNP showed the changes in its secondary structure; such changes include the decomposition of α-helix strand and the increase in β-pleated sheet and random coil percentages. Fluorescence study indicated minimal changes in the tertiary structure near the microenvironment of the aromatic amino acids such as tyrosine and phenyl alanine caused by the interaction forces between the CaPNP and hCG protein. The desorption process showed that the quantity of the hCG desorbed significantly increases as temperature increases, which indicates the weak forces between hCG and the surface.

  10. Expression of Human Chorionic Gonadotropin β (hCGβ) in Lactobacillus Casei

    Institute of Scientific and Technical Information of China (English)

    Hong-mei WANG; Hong JIANG; Zheng-hong YUAN; Da-jin LI

    2003-01-01

    Objective To construct a recombinant lactobacillus (Lb.) strain excreting the human chorionic gonadotropin beta-subunit, hCGβMethods The hCGβ cDNA was ligated to the signal peptide sequence of S-layer protein from Lb. brevis and then cloned into down-stream of lactose-inducible promoter of an integrative plasmid, pIlac. After electroporation into Lb. casei CECT5276, PCR using the genomic DNA of the recombinant lactobacillus as template was performed to confirm whether the hCGβ gene had been integrated into the genome. Radioimmunoassay (RIA) was used to determine the level of hCGβ in the supernatant and the cell lysate. Results The hCGβ was integrated into the genome of Lb. casei CECT5276. The highest concentration of hCGβ in the culture supernatant amounted to 440 mIU/mL 21 h after lactose induction. About 2/3 of the objective proteins were excreted into the supernatant.Conclusion We have obtained stable and efficient hCGβ excretion in Lb. casei, which was inducible by lactose.

  11. Polymer nanoparticles as fluorescent labels in a fluoroimmunoassay for human chorionic gonadotropin

    International Nuclear Information System (INIS)

    A sensitive fluoroimmunoassay (FIA) was developed for the determination of human chorionic gonadotropin (β-HCG). It is based on fluorescent polymer nanoparticles (PFNPs) coated with anti-β-HCG monoclonal antibodies in a sandwich type of fluoroimmunoassay. The PFNPs were synthesized by precipitation polymerization using methacrylic acid (MAA) as the monomer, trimethylolpropane trimethacrylate as the cross-linker, azobisisobutyronitrile as the radical initiator, and fluorescein as the fluorophore. Anti-β-HCG monoclonal antibody was labeled with the PFNPs and then used in a FIA of β-HCG in human serum samples using low-fluorescent transparent 96-well microtiter plates. The calibration graph for β-HCG is linear over the range from 1. 25 to 300 mIU mL-1 with a detection limit of 0. 3 mIU mL-1 (3σ). The relative standard deviation for seven parallel measurements of 10 mIU mL-1 of β-HCG is 3. 8%. The method has the specificity of an immunoassay and the sensitivity of fluorescent nanoparticle label technology. (author)

  12. Analysis of human chorionic gonadotropin-monoclonal antibody interaction in BIAcore

    Indian Academy of Sciences (India)

    Banerjee Ashish; Gundlupet Satyanarayana Murthy

    2004-03-01

    Kinetic studies of macromolecular ligand-ligate interaction have generated ample interest since the advent of plasmon resonance based instruments like BIAcore. Most of the studies reported in literature assume a simple 1 : 1 Langmuir binding and complete reversibility of the system. However we observed that in a high affinity antigen-antibody system [human chorionic gonadotropin-monoclonal antibody (hCG-mAb)] dissociation is insignificant and the sensogram data cannot be used to measure the equilibrium and kinetic parameters. At low concentrations of mAb the complete sensogram could be fitted to a single exponential. Interestingly we found that at higher mAb concentrations, the binding data did not conform to a simple bimolecular model. Instead, the data fitted a two-step model, which may be because of surface heterogeneity of affinity sites. In this paper, we report on the global fit of the sensograms. We have developed a method by which a single two-minute sensogram can be used in high affinity systems to measure the association rate constant of the reaction and the functional capacity of the ligand (hCG) immobilized on the chip. We provide a rational explanation for the discrepancies generally observed in most of the BIAcore sensograms.

  13. Value of human chorionic gonadotropin compared to CEA in discriminating benign from malignant effusions.

    Science.gov (United States)

    Lamerz, R; Stoetzer, O J; Mezger, J; Brandt, A; Darsow, M; Wilmanns, W

    1999-01-01

    Human chorionic gonadotropin (HCG) is expressed in germ cell tumors and urothelial, breast, lung and colon cancers. The aim of the study was to investigate if the determination of HCG in comparison with CEA is able to discriminate between malignant and benign effusions. Effusion and partially serum samples of 61 patients with benign (g.i., heart/kidney isnuff.) and 116 patients with malignant diseases (g.i., gynec., lung, misc., CUP) were investigated. HCG was specifically determined by an IRMA using 2 monoclonal antibodies, CEA by a conventional double Ab RIA. Cytological staining was preformed using the Pappenheim-method on cytospin preparations. Significant differences (p gynec. (32.1%) gynec. (60%) pleural effusions with high specificity (90% at 5 IU/l) but low sensitivity of 31% increasing in g.i., lung and gynecologic cases, CEA a more general TM with higher sensitivity of 45% increasing in g.i., gynecologic and lung cases (sp. 100% at 9 ng/ml) both adding significantly to cytology-negative effusions. PMID:10470169

  14. Effect of Adding Human Chorionic Gonadotropin to The Endometrial Preparation Protocol in Frozen Embryo Transfer Cycles

    Directory of Open Access Journals (Sweden)

    Maryam Eftekhar

    2012-01-01

    Full Text Available Background: Human chorionic gonadotropin (HCG, one of the initial embryonic signals, isprobably a major regulator of the embryo-endometrial relationship. This study aims to assess theadvantage of HCG supplementation during the secretory phase of hormonally prepared cycles forthe transfer of cryopreserved-thawed embryos.Materials and Methods: This study was a randomized clinical trial. Infertile women who werecandidates for frozen-thawed embryo transfers entered the study and were divided into two groups,HCG and control. The endometrial preparation method was similar in both groups: all women receivedestradiol valerate (6 mg po per day from the second day of the menstrual cycle and progesteronein oil (100 mg intramuscular (I.M. when the endometrial thickness reached 8 mm. Estradiol andprogesterone were continued until the tenth week of gestation. In the HCG group, patients received anHCG 5000 IU injection on the first day of progesterone administration and the day of embryo transfer.Results: In this study, 130 couples participated: 65 in the HCG group and 65 in the control group.There was no statistically significant difference between groups regarding basic characteristics.Implantation rate, chemical pregnancy, clinical pregnancy, ongoing pregnancy, and abortion rateswere similar in both groups.Conclusion: Although HCG has some advantages in assisted reproductive technology (ARTcycles, our study did not show any benefit of HCG supplementation during the secretory phase offrozen cycles (Registration Number: IRCT201107266420N4.

  15. Ovarian reaction and estrus manifestation in delayed puberty gilts after treatment with equine chorionic gonadotropin

    Directory of Open Access Journals (Sweden)

    Stančić Ivan B

    2012-08-01

    Full Text Available Abstract Background Prolonged pre-insemination anestrus (i.e. delayed puberty is a major contributing factor for culling up to 30% of the replacement gilts at large breeding farm units in Vojvodina. It is imperative to determine if these gilts are acyclic (prepubertal or cyclic, but just fail to exhibit behavioural estrus. Recent investigations demonstrate that treatment with equine chorionic gonadotropin (eCG can increase the diestrous phase duration in sexually mature gilts. Based on these finding, the aim of the present studies was to determine the reproductive status of delayed puberty gilts following injection with eCG. Methods Two experiments were conducted on a swine breeding farm in Vojvodina. In Exp. 1, 20 prepubertal (acyclic gilts, and 120 sexually mature (cyclic gilts were injected with a single injection of 400 IU eCG + 200 IU human chorionic gonadotropin (hCG or with 1000 IU eCG (cyclic gilts, at d5, d11 or d17 after spontaneous estrus detection, to determine their ovarian reaction and induced estrus manifestation. In Exp. 2, sixty delayed puberty gilts (estrus not detected until 8 month of age, av. 258 days were culled from breeding herd and slaughtered to determine their reproductive status based on ovarian anatomical features. The second group of gilts (n = 60 was treated with a single 1000 IU eCG injection to determine their reproductive status, based on the interval between eCG injection to estrus detection and duration. The data were analyzed by descriptive statistics, t-test, analysis of variance and Duncan’s test in the software package Statistics 10th. Results Ovulations were induced in 90% of acyclic (sexually immature and, on average, 93.3% of cyclic (sexually mature gilts after the eCG injection. On average, 4 days after the eCG injection, estrus was detected in 85% of the treated acyclic (sexually immature gilts and in 95% (19/20 of the cyclic (sexually mature gilts, treated with eCG on day 17

  16. Structural analysis of gubernaculum testis in cryptorchid patients submitted to treatment with human chorionic gonadotrophin

    Directory of Open Access Journals (Sweden)

    Charbel S. El Zoghbi

    2007-04-01

    Full Text Available OBJECTIVES: To make a histological and stereological analysis of gubernaculum testis elastic system fibers, collagen and striated muscle cells in patients with cryptorchidism treated with human chorionic gonadotrophin (hCG. MATERIALS AND METHODS: Gubernacula tissue samples were obtained from 12 patients with cryptorchidism. Patients' ages ranged from 1 to 3 years (mean 1.8. Of the 12 patients, 6 were treated with hCG. The samples were stained with Masson's trichrome to highlight muscle fibers and collagen, and Weigert's resorcin-fuchsin to highlight the elastic system fibers. The volumetric density of these components was determined by stereological methods. RESULTS: The volumetric density of collagen was increased in patients treated with hCG, ranging from 85.62% to 94.48%, while in patients not submitted to hCG treatment the volume density ranged from 52.12% to 89.46% (p = 0.0052. The volumetric density of the elastic system fibers was higher in patients treated with hCG, ranging from 9.62% to 19.62%, while patients not submitted to hCG treatment the volume density of elastic system fibers was between 10.38% and 12.38% (p = 0.0030. The volume density of striated muscle fibers in patients treated with hCG ranged from 4.76% to 39.16%, while and in patients not treated hCG it ranged from 3.24% to 11.14% (p = 0.0052. CONCLUSION: Gubernacular components alter significantly when submitted to treatment with hCG. Patients who underwent hCG treatment and had no complete testicular migration had an increase in the concentration of elastic and striated muscle fibers and a decrease in the volumetric density of collagen.

  17. Serum human chorionic gonadotropin is associated with angiogenesis in germ cell testicular tumors

    Directory of Open Access Journals (Sweden)

    Avilés-Salas Alejandro

    2009-08-01

    Full Text Available Abstract Background Germ cell testicular tumors have survival rate that diminishes with high tumor marker levels, such as human chorionic gonadotropin (hCG. hCG may regulate vascular neoformation through vascular endothelial growth factor (VEGF. Our purpose was to determine the relationship between hCG serum levels, angiogenesis, and VEGF expression in germ cell testicular tumors. Methods We conducted a retrospective study of 101 patients. Serum levels of hCG, alpha-fetoprotein (AFP, and lactate dehydrogenase were measured prior to surgery. Vascular density (VD and VEGF tissue expression were determined by immunohistochemistry and underwent double-blind analysis. Results Histologically, 46% were seminomas and 54%, non-seminomas. Median follow-up was 43 ± 27 months. Relapse was present in 7.5% and mortality in 11.5%. Factors associated with high VD included non-seminoma type (p = 0.016, AFP ≥ 14.7 ng/mL (p = 0.0001, and hCG ≥ 25 mIU/mL (p = 0.0001. In multivariate analysis, the only significant VD-associated factor was hCG level (p = 0.04. When hCG levels were stratified, concentrations ≥ 25 mIU/mL were related with increased neovascularization (p Conclusion This is the first study that relates increased serum hCG levels with vascularization in testicular germ cell tumors. Hence, its expression might play a role in tumor angiogenesis, independent of VEGF expression, and may explain its association with poor prognosis. hCG might represent a molecular target for therapy.

  18. Expression of human chorionic gonadotropin beta in gastric carcinoma: A retrospective immunohistochemical study

    Directory of Open Access Journals (Sweden)

    Murhekar Kanchan

    2009-01-01

    Full Text Available Context: Beta Human Chorionic Gonadotropin (βHCG, a marker of the trophoblastic neoplasm, is also secreted by non-trophoblastic neoplasms including gastric carcinomas. Its role in disease progression remains unclear. Aim: To investigate the incidence of βHCG positivity in gastric carcinomas and correlate its presence with the biological behavior of the tumor. Setting and Design: A hospital-based, immunohistochemical study. Materials and Methods: One hundred and fifty formalin-fixed, paraffin-embedded tissue specimens from histopathologically confirmed cases of gastric carcinoma were immunostained using an indigenously developed antibody against βHCG. Tumors with diffuse reactivity to βHCG were considered as positive. Those with occasional, focal or no reactivity to βHCG were considered as negative. Statistical Analysis: Differences in βHCG staining were compared according to the histological grade and surgical stage using the χ2 test. Using the Cox proportional hazards model, the time till the onset of development of an adverse outcome after surgery (defined as death, local or distant metastasis was compared between the bHCG positive and negative tumors. Results: Twenty-eight (18.7% of the 150 specimens were βHCG positive. No association was found between the histological grade (P=0.49 and the surgical stage (P=0.19 with βHCG positivity. The median disease-free survival after surgery was not different among bHCG positive and negative tumors. Risk of an adverse outcome after surgery was significantly associated with the stage of the tumor (Hazard ratio=2.9, 95% confidence interval: 1.1-7.4. No association was observed with grade or βHCG positivity. Conclusion: βHCG immunoreactivity was observed in about one-fifth of the gastric cancers. bHCG reactivity, however, played no role in the biological behavior.

  19. Evaluation of melengestrol acetate and equine chorionic gonadotropin for out-of-season breeding in sheep on Prince Edward Island.

    OpenAIRE

    Keefe, G P; Wichtel, J J

    2000-01-01

    The purpose of this study was to compare the efficacy of a recommended protocol of oral melengestrol acetate (MGA) to intravaginal medroxyprogesterone acetate (MPA), with or without equine chorionic gonadotropin (eCG), for out-of-season breeding of sheep on Prince Edward Island. One hundred and twenty ewes were assigned to 1 of 4 groups in a factorial design and were treated with either an intravaginal MPA sponge or oral MGA. Ewes received either an intramuscular injection of eCG or a saline ...

  20. Separation of beta-human chorionic gonadotropin and immunoglobulin G by a miniaturized size exclusion chromatography column

    Science.gov (United States)

    Yang, Yongmo; Chae, Junseok

    2009-04-01

    This report describes a miniaturized size exclusion chromatography column that effectively preseparates raw samples for medical point-of-care testing (POCT) devices. The minicolumn is constructed of polydimethylsiloxane fabricated on a glass slide. The minicolumn separates 300 ng/ml of beta-human chorionic gonadotropin (β-hCG) from an immunoglobulin G (IgG)-rich solution (100 μg/ml) in 7.7 min, with 2.23 resolution and 0.018 mm plate height. The complete analyte discrimination shows potential for the sample preparation stage of POCT devices for cancer screening, prognosis, and monitoring.

  1. Human chorionic gonadotropin and implantation%人绒毛膜促性腺激素与胚胎种植

    Institute of Scientific and Technical Information of China (English)

    李洁; 陈晓燕; 徐少元

    2013-01-01

    Human chorionic gonadotropin(HCG)is a glycoprotein hormone comprised of 2 subunits a and b that are noncovalently joined. HCG is primarily produced by the embryo and later by the syncytiotrophoblast. HCG has been detected at variable levels both in pre-implantation embryo culture media in vitro and in women serum after fertilization around implantation period or early pregnancy. HCG is not only considered to assess embryo competence both in anatomic and biochemical,but also contributes to improve luteal function and endometrial modification or placenta construction for supporting early pregnancy.

  2. Human chorionic gonadotropin: Different glycoforms and biological activity depending on its source of production.

    Science.gov (United States)

    Fournier, Thierry

    2016-06-01

    Human chorionic gonadotropin (hCG) is the first hormonal message from the placenta to the mother. It is detectable in maternal blood two days after implantation and behaves like a super LH agonist stimulating progesterone secretion by the corpus luteum. In addition to maintaining the production of progesterone until the placenta itself produces it, hCG also has a role in myometrial quiescence and local immune tolerance. Specific to humans, hCG is a complex glycoprotein composed of two highly glycosylated subunits. The α-subunit is identical to the pituitary gonadotropin hormones (LH, FSH, TSH), contains two N-glycosylation sites, and is encoded by a single gene (CGA). By contrast, the β-subunits are distinct for each hormones and confer both receptor and biological specificity, although LH and hCG bind to the same receptor (LH/CG-R). The hCG ß-subunit is encoded by a cluster of genes (CGB) and contains two sites of N-glycosylation and four sites of O-glycosylation. The hCG glycosylation state varies with the stage of pregnancy, its source of production and in the pathology. It is well established that hCG is mainly secreted into maternal blood, where it peaks at 8-10weeks of gestation (WG), by the syncytiotrophoblast (ST), which represents the endocrine tissue of the human placenta. The invasive extravillous trophoblast (iEVT) also secretes hCG, and in particular hyperglycosylated forms of hCG (hCG-H) also produced by choriocarcinoma cells. In maternal blood, hCG-H is elevated during early first trimester corresponding to the trophoblastic cell invasion process and then decreases. In addition to its endocrine role, hCG has autocrine and paracrine roles. It promotes formation of the ST and angiogenesis through LH/CG-R but has no effect on trophoblast invasion in vitro. By contrast, hCG-H stimulates trophoblast invasion and angiogenesis by interacting with the TGFß receptor in a LH/CG-R independent signalling pathway. hCG is largely used in antenatal screening

  3. New discoveries on the biology and detection of human chorionic gonadotropin

    Directory of Open Access Journals (Sweden)

    Cole Laurence A

    2009-01-01

    Full Text Available Abstract Human chorionic gonadotropin (hCG is a glycoprotein hormone comprising 2 subunits, alpha and beta joined non covalently. While similar in structure to luteinizing hormone (LH, hCG exists in multiple hormonal and non-endocrine agents, rather than as a single molecule like LH and the other glycoprotein hormones. These are regular hCG, hyperglycosylated hCG and the free beta-subunit of hyperglycosylated hCG. For 88 years regular hCG has been known as a promoter of corpus luteal progesterone production, even though this function only explains 3 weeks of a full gestations production of regular hCG. Research in recent years has explained the full gestational production by demonstration of critical functions in trophoblast differentiation and in fetal nutrition through myometrial spiral artery angiogenesis. While regular hCG is made by fused villous syncytiotrophoblast cells, extravillous invasive cytotrophoblast cells make the variant hyperglycosylated hCG. This variant is an autocrine factor, acting on extravillous invasive cytotrophoblast cells to initiate and control invasion as occurs at implantation of pregnancy and the establishment of hemochorial placentation, and malignancy as occurs in invasive hydatidiform mole and choriocarcinoma. Hyperglycosylated hCG inhibits apoptosis in extravillous invasive cytotrophoblast cells promoting cell invasion, growth and malignancy. Other non-trophoblastic malignancies retro-differentiate and produce a hyperglycosylated free beta-subunit of hCG (hCG free beta. This has been shown to be an autocrine factor antagonizing apoptosis furthering cancer cell growth and malignancy. New applications have been demonstrated for total hCG measurements and detection of the 3 hCG variants in pregnancy detection, monitoring pregnancy outcome, determining risk for Down syndrome fetus, predicting preeclampsia, detecting pituitary hCG, detecting and managing gestational trophoblastic diseases, diagnosing quiescent

  4. Early gestation chorionic villi-derived stromal cells for fetaltissue engineering

    Institute of Scientific and Technical Information of China (English)

    Lee Lankford; Taryn Selby; James Becker; Volodymyr Ryzhuk; Connor Long; Diana Farmer; Aijun Wang

    2015-01-01

    AIM To investigate the potential for early gestationplacenta-derived mesenchymal stromal cells (PMSCs) forfetal tissue engineering.METHODS: PMSCs were isolated from early gestationchorionic villus tissue by explant culture. Chorionic villussampling (CVS)-size tissue samples (mean = 35.93 mg)were used to test the feasibility of obtaining large cellnumbers from CVS within a clinically relevant timeframe.We characterized PMSCs isolated from 6 donor placentasby flow cytometry immunophenotyping, multipotencyassays, and through immunofluorescent staining. Proteinsecretion from PMSCs was examined using two cytokinearray assays capable of probing for over 70 factorsin total. Delivery vehicle compatibility of PMSCs wasdetermined using three common scaffold systems: fibringlue, collagen hydrogel, and biodegradable nanofibrousscaffolds made from a combination of polylactic acid (PLA)and poly(lactic-co-glycolic acid) (PLGA). Viral transductionof PMSCs was performed using a Luciferase-GFPcontaininglentiviral vector and efficiency of transductionwas tested by fluorescent microscopy and flow cytometryanalysis.RESULTS: We determined that an average of 2.09 ×106 (SD ± 8.59 × 105) PMSCs could be obtained fromCVS-size tissue samples within 30 d (mean = 27 d, SD± 2.28), indicating that therapeutic numbers of cells canbe rapidly expanded from very limited masses of tissue.Immunophenotyping by flow cytometry demonstratedthat PMSCs were positive for MSC markers CD105,CD90, CD73, CD44, and CD29, and were negative forhematopoietic and endothelial markers CD45, CD34,and CD31. PMSCs displayed trilineage differentiationcapability, and were found to express developmentaltranscription factors Sox10 and Sox17 as well as neuralrelatedstructural proteins NFM, Nestin, and S100β.Cytokine arrays revealed a robust and extensive profileof PMSC-secreted cytokines and growth factors, anddetected 34 factors with spot density values exceeding103. Detected factors

  5. The Influences of Mifepristone, Norethisterone and Tamoxifen on the Glycosphingolipids Compositions from Human Chorionic Tissue during Early Pregnancy

    Institute of Scientific and Technical Information of China (English)

    孙翠翔; 周剑萍; 于传鑫; 张俊慧; 张厦英; 刘银刊

    1996-01-01

    By using the Ladisch partitioning and microscale-analysis of HPTLC the comparative quantitative and qualitative studies of gangtiosides (Gg) and neutral gtycosphingotipids ( N-GSL ) compositions from human chorionic villi tissues of normal early pregnant women and women treated with mifepristone, norethisterone (NET) and tamoxifen (TAM) were reported in this paper. The patterns of Gg and N-GSL in three treated groups were similar to those in normal pregnant group, The total values of Gg from the ckorionic villi tissues reduced significantly in three treated groups (P<0. 01), In all treated groups, the amounts of NeuNAC-Gal-Glc-cer (GM3) , NeuNAC-NeuNAC-Gal-Gtc-cer ( GD3) and Gal-GalNAC-(NeuNAC)-Gal-Glc-eer(GM1 ) were decreased significantly compared with those in normal (P<0. 01 or P<0. 05), In NET and TAM groups, Neu NAC-Gal-GalNAC-(NeuNAC-NeuNAC )-Gal-Glc-cer( GT16 ) was markedly lower than that in normal (P<0. 01), The total values of N-GSL extracted from the chorionic villi tissues were obviously higher in mifepristone and TAM groups than those in normal (P<0. 01). The Gal-Gte-cer(LacCer) (CDH) and Gal-Gal-Glc-cer(Gat-LacCer) (CTH)were greatly increased in mifepristone group as compared with normal (P<0. 05 ), Paragloboside : Gat-GalNAC-Gal-Gtc-cer (PG) in NETgroup was significantly higher than that in normal (P<0. 01 ).

  6. A thicker chorion gives ova of Atlantic salmon (Salmo salar L.) the upper hand against Saprolegnia infections.

    Science.gov (United States)

    Songe, M M; Willems, A; Sarowar, M N; Rajan, K; Evensen, Ø; Drynan, K; Skaar, I; van West, P

    2016-07-01

    Since the ban of malachite green in the fish farming industry, finding alternative ways of controlling Saprolegnia infections has become of utmost importance. Much effort has been made to elucidate the mechanisms by which Saprolegnia invades fish eggs. Little is known about the defence mechanisms of the hosts, making some eggs more prone to infection than others. One clue might lie in the composition of the eggs. As the immune system in the embryos is not developed yet, the difference in infection levels could be explained by factors influenced by the mother herself, by either transferring passive immunity, influencing the physical aspects of the eggs or both. One of the physical aspects that could be influenced by the female is the chorion, the extracellular coat surrounding the fish egg, which is in fact the first major barrier to be overcome by Saprolegnia spp. Our results suggest that a thicker chorion in eggs from Atlantic salmon gives a better protection against Saprolegnia spp. In addition to the identification of differences in sensitivity of eggs in a fish farm set-up, we were able to confirm these results in a laboratory-controlled challenge experiment. PMID:26644366

  7. First trimester pregnancy-associated plasma protein A and human chorionic gonadotropin-beta in early and late pre-eclampsia

    DEFF Research Database (Denmark)

    Karahasanovic, Azra; Sørensen, Steen; Nilas, Lisbeth

    2014-01-01

    Abstract Background: The aim of this study was to compare pregnancy-associated plasma protein A (PAPP-A) and the β-subunit of human chorionic gonadotropin (hCGβ) measured in maternal plasma at the first trimester screening, in women who later developed early or late pre-eclampsia (PE) to women...

  8. False negative NIPT results: Risk figures for chromosomes 13,18 and 21 based on chorionic villi results in 5967 cases and literature review

    NARCIS (Netherlands)

    A.R.M. van Opstal (Diane); M. Srebniak (Malgorzata); J. Polak (Joke); F.A.T. de Vries (Femke); L.C. Govaerts (Lutgarde); M. Joosten (Marieke); A. Go (Attie); M.F.C.M. Knapen (Maarten); C.D.F. van den Berg (Cardi); K.E.M. Diderich (Karin); R-J.H. Galjaard (Robert-Jan)

    2016-01-01

    textabstractNon-invasive prenatal testing (NIPT) demonstrated a small chance for a false negative result. Since the "fetal" DNA in maternal blood originates from the cytotrophoblast of chorionic villi (CV), some false negative results will have a biological origin. Based on our experience with cytog

  9. DETECTION OF STRAND BREAKS OF DNA IN HUMAN EARLY CHORIONIC VILLUS CELLS INDUCED BY DIAGNOSTIC ULTRASOUND USING 32p-LABELED ALU HYBRIDIZATION

    Institute of Scientific and Technical Information of China (English)

    Wang Caifeng; Li Xu; Zhang Yunjing

    2006-01-01

    Objective To explore if strand breaks of DNA in human early chorionic villus cells in uterus were induced by diagnostic ultrasound and to evaluate the method used for detection of single-stranded breaks and doublestranded breaks in human DNA. Methods 60 normal pregnant women aged 20-30, who underwent artificial abortion during 6-8 weeks of gestation, were randomly divided into 2 experimental groups: All 30 cases were exposed to diagnostic ultrasound in uterus for 10 minutes, and 24 hours later chorionic villi were extracted; the other 30 cases were taken as the control group. Single-stranded DNA and double-stranded DNA in villus cells in all cases were isolated by the alkaline unwinding combined with hydroxylapatite chromatography, and were quantitatively detected using32 P-labeled Alu probe for dot-blotting hybridization. Results There was no significant difference in quantity and percentage in single-stranded DNA and double-stranded DNA between 2 groups (P>0.05). 32 P-Alu probe could only hybridize with human DNA, and could detect DNA isolated from as few as 2.5 × 103 chorionic villus cells and 0.45 ng DNA in human leukocytes. Conclusion The results suggested that there were no DNA strand damages in human chorionic villus cells when the uterus was exposed to diagnostic ultrasound for 10 minutes. The method, 32P-Alu probe for dot-blotting hybridization, was even more specific, sensitive and accurate than conventional approaches.

  10. A peptide mimic of an antigenic loop of alpha-human chorionic gonadotropin hormone: solution structure and interaction with a llama V-HH domain

    NARCIS (Netherlands)

    Ferrat, G.; Renisio, J.G.; Morelli, X.; Slootstra, J.W.; Meloen, R.; Cambillau, C.; Darbon, H.

    2002-01-01

    The X-ray structure of a ternary complex between human chorionic gonadotropin hormone (hCG) and two Fvs recognizing its alpha and beta subunits has been recently determined. The Fvs recognize the elongated hCG molecule by its two ends, one being the Leu-12-Cys-29 loop of the alpha subunit. We have d

  11. Amniotic membrane is a potential regenerative option for chronic non-healing wounds: a report of five cases receiving dehydrated human amnion/chorion membrane allograft.

    Science.gov (United States)

    Mrugala, Andrew; Sui, Audrey; Plummer, Malgorzata; Altman, Igor; Papineau, Elaine; Frandsen, Devn; Hill, Danielle; Ennis, William J

    2016-08-01

    A case series of five patients with a total of six chronic non-healing wounds (>30 day duration) were non-randomly selected to evaluate the performance, safety and handling properties of dehydrated human amnion/chorion membrane allograft, an amniotic membrane scaffolding product. The patients had lower extremity wounds that had previously failed standard of care within a university outpatient/inpatient wound healing programme. Five wounds treated with dehydrated amnion/chorion membrane allograft showed a mean 43% area reduction from baseline (51% median) at 3 weeks into treatment and completely healed with a 64-day median time to closure (SD ±27·6 days). One wound worsened at 3 weeks and was found to have a complete central vein obstruction that was treated with long-term mild compression but still eventually healed at 6 months. Removing this outlier, the four responding wounds had a 72% mean and 69% median change in area from baseline, at the 3 week point. All five patients received only one application of dehydrated human amnion/chorion membrane allograft, and there were no adverse events. The product was easy to use, administer and handle. In summary, dehydrated human amnion/chorion membrane allograft appears to be a safe, effective and easy to use therapy for chronic non-healing wounds. This study describes the details of these clinical cases and provides an overview of the current evidence on the use of amniotic tissue in clinical practice. PMID:25974156

  12. Treatment of cryptorchidism with human chorionic gonadotropin or gonadotropin releasing hormone. A double-blind controlled study of 243 boys

    DEFF Research Database (Denmark)

    Christiansen, P; Müller, J; Buhl, S;

    1988-01-01

    We have conducted a modified double-blind study on the effect of human chorionic gonadotropin (hCG), gonadotropin releasing hormone (GnRH) and placebo on bilateral and unilateral maldescended testes. One hundred and fifty-five boys with bilateral and 88 boys with unilateral cryptorchidism fulfilled...... = 0.0009). Both testes descended in 25% of the boys following treatment with hCG, and improvement in the position of the testes was obtained in a further 25% of the cases. hCG administration resulted in complete testicular descent in 14% of boys with unilateral cryptorchidism compared with 3 and 0...... the use of hCG in the treatment of maldescended testes, whereas the study did not support a general use of GnRH administered intranasally....

  13. Inhibin and activin modulate the release of gonadotropin-releasing hormone, human chorionic gonadotropin, and progesterone from cultured human placental cells.

    OpenAIRE

    Petraglia, F; Vaughan, J.; Vale, W

    1989-01-01

    Although it is clear that human chorionic gonadotropin (hCG) and progesterone play fundamental roles in pregnancy, the regulation of placental production of these hormones remains to be defined. Recent evidence suggests that the human placenta expresses proteins related to inhibin (alpha beta subunits) or activin (beta beta subunits). Inhibin and activin (follicle-stimulating hormone-releasing protein) possess opposing activities in several biological systems including pituitary follicle-stim...

  14. Development of Some Organs Derived from the Three Embryonic Germ Layer in a Degus Ectopic Pregnancy and Presence of a Cytotrophoblast That Mimics Human Chorionic Placenta

    Directory of Open Access Journals (Sweden)

    C. Bosco

    2014-01-01

    Full Text Available This report describes a case of abdominal pregnancy in an adult female degu from which we recovered two large tissular masses from the peritoneal cavity. The bigger one showed a number of thin vascular connections to the serosa layer of the small intestine. It was also directly connected to the smaller mass by a thin membranous process. The surface of the bigger mass facing the small intestine wall showed the presence of chorionic villous that resembled a villous human chorionic placenta, rather than the hemomonochorial labyrinthine placenta, characteristic of this species. This unusual finding leads us to postulate that in the degu’s uterus the cytotrophoblast is exposed to a number of factors that will activate cascades of cellular and molecular events that ultimately will be signaling the cytotrophoblast to develop into a labyrinthine hemomonochorial placenta. In absence of the proper uterine environment, as is the case of the abdominal pregnancy in the peritoneal cavity reported here, the lack of signaling will lead the cytotrophoblast to develop into a villous chorionic placenta, similar to that observed in human.

  15. The Expression of Integrin β3 and Intercellular Adhesion Molecule(ICAM-1)in Decidua and Chorionic Villi during Mifepristone Induced Abortion

    Institute of Scientific and Technical Information of China (English)

    李瑞珍; 王振海; 吴瑞芳

    1999-01-01

    The effects of mifepristone with misoprostol on the expression of the integrin β3 and intercellular adhesion motecule-1 (ICAM-1)in decidua and chorionic villi tissues in early pregnancy in 10 cases were investigated by immuno-ftow cytometry(the eyper-iment group).At the same time,the other 10 cases induced by mechanical vacuum as-piration were collected as the control.The results showed that,the positive rate of inte-grin β3 and ICAM-1 in decidua of the experiment group were 19.1±5. 01% and 20.61±6. 51%;while those in chorionic villi were 21.32±4. 38% and 20. 29±6. 49%,which were significantly lower than those in the control group.These results suggested that integrin β3 and ICAM-1 may take part in the maintenance of early pregnancy.The mechanism of mifepristone induced abortion may be mediated by the down-regulation of the integrin β3 and ICAM-1 expression in decidua and chorionic villi.

  16. Expression of Beta-Human Chorionic Gonadotropin Genes in Renal Cell Cancer and Benign Renal Disease Tissues

    Institute of Scientific and Technical Information of China (English)

    姜永光; 曾甫清; 肖传国; 刘俊敏

    2003-01-01

    To study the expression of beta-human chorionic gonadotropin (βhCG) genes in renal cellcarcinomas (RCC) and benign renal disease tissues, nested reverse transcription-polymerase chainreaction (RT-PCR) and restriction endonuclease analysis were employed to detect the expression ofβhCG genes in 44 cases of RCC tissues and 24 cases of benign renal disease tissues. It was foundthat 52% RCC samples revealed positive for βhCG mRNA expression. Positive rate in advancedstage and poorly differentiated RCC was higher, but there was no significant difference. The posi-tive rate of βhCG mRNA expression was 54% in 24 cases of benign renal tissues, including 3 casesout of 6 polycystic kidneys, 7 cases out of 13 renal atrophies, 2 cases out of 2 oncocytomas and 1case out of 2 pyonephrotic kidneys. β7 was most frequently transcribed subtype gene independent onthe histology. These findings suggested βhCG gene transcription is not only involved in RCC but al-so in benign renal diseases.

  17. A controlled study of human chorionic gonadotrophin induced ovulation versus urinary luteinizing hormone surge for timing of intrauterine insemination.

    Science.gov (United States)

    Martinez, A R; Bernadus, R E; Voorhorst, F J; Vermeiden, J P; Schoemaker, J

    1991-10-01

    Forty-eight patients in a programme of intrauterine insemination (IUI) were randomized in a cross-over study. All were stimulated with clomiphene citrate (CC) and inseminated either after follicular rupture induced by human chorionic gonadotrophin (HCG) or after a spontaneous urinary luteinizing hormone (LH) surge. The HCG was administered when follicles of 18-22 mm in diameter were observed on ultrasound and IUI was performed 37-40 h thereafter. The monitoring of a urinary LH peak was carried out using a rapid urinary LH test. IUI took place approximately 22 h after detection of the LH surge. Overall, the pregnancy rates were 9.3% (4/43) after HCG induced ovulation and 20.5% (9/44) after spontaneous ovulation (P = 0.12). Analysis of mid-cycle events showed that following sonographic criteria, the HCG injection was performed significantly earlier in the cycle compared with the spontaneous LH surge. In addition, the mean diameter of the preovulatory follicles was significantly smaller and insemination was substantially earlier in the HCG induced cycles. These findings suggest that a beneficial effect arises from allowing the natural process of final follicular maturation to occur. PMID:1752926

  18. Seasonal changes and human chorionic gonadotrophin (hCG) effects on innate immune genes expression in goldfish (Carassius auratus).

    Science.gov (United States)

    Zhong, Huan; Zhou, Yi; Yu, Fan; Xiao, Jun; Gan, Xi; Zhang, Ming

    2014-06-01

    We profiled the expression of a group of proinflammatory immune genes, comprising TNFα-1, TNFα-2, IFN-γ, IL1β-1, IL1β-2, CCL-1, and CXCL-8 in liver, head kidney, gills, and spleen of goldfish, during the reproductive cycle and in response to injection of the hormone human chorionic gonadotrophin (hCG). Most genes showed higher expression during the breeding season in both sexes. However, activation of immune responses was much stronger in female goldfish. Injection with hCG, an analog of luteinizing hormone (LH), which is involved in numerous reproductive functions, markedly changed gene expression in most studied organs, in both male and female goldfish. Again, female goldfish were found to be more responsive than male goldfish. The strongest activation of these genes was seen 7 days post-injection; the effect was dose dependent with a lower dose being in general more effective. For several of the genes, the gills were the most responsive tissue and, in male goldfish, gills were often the only responsive tissue, suggesting an important immunological role for gills during breeding. The data suggest that increasing expression levels are regulated by LH arising during the breeding season, with greater sensitivity in female goldfish than in male goldfish. These data support an interaction between the innate immune system and the reproductive axis. PMID:24709628

  19. Regenerative and reparative effects of human chorion-derived stem cell conditioned medium on photo-aged epidermal cells.

    Science.gov (United States)

    Li, Qiankun; Chen, Yan; Ma, Kui; Zhao, Along; Zhang, Cuiping; Fu, Xiaobing

    2016-01-01

    Epidermal cells are an important regenerative source for skin wound healing. Aged epidermal cells have a low ability to renew themselves and repair skin injury. Ultraviolet (UV) radiation, particularly UVB, can cause photo-aging of the skin by suppressing the viability of human epidermal cells. A chorion-derived stem cell conditioned medium (CDSC-CNM) is thought to have regenerative properties. This study aimed to determine the regenerative effects of CDSC-CNM on UVB-induced photo-aged epidermal cells. Epidermal cells were passaged four times and irradiated with quantitative UVB, and non-irradiated cells served as a control group. Cells were then treated with different concentrations of CDSC-CNM. Compared to the non-irradiated group, the proliferation rates and migration rates of UVB-induced photo-aged epidermal cells significantly decreased (p photo-aged epidermal cells significantly improved their viability, and their ROS generation and DNA damage decreased. The secretory factors in CDSC-CNM, including epidermal growth factor (EGF), transforming growth factor-β (TGF-β), interleukin (IL)-6, and IL-8 and the related signaling pathway protein levels, increased compared to the control medium (CM). The potential regenerative and reparative effects of CDSC-CNM indicate that it may be a candidate material for the treatment of prematurely aged skin. The functions of the secretory factors and the mechanisms of CDSC-CNM therapy deserve further attention.

  20. Predictive value of early serum beta-human chorionic gonadotrophin for the successful outcome in women undergoing in vitro fertilization

    Directory of Open Access Journals (Sweden)

    Neeta Singh

    2013-01-01

    Full Text Available Aims: Pregnancies achieved by in vitro fertilization (IVF are at increased risk of adverse outcome. The main objective of this study was to evaluate the predictive value of β-human chorionic gonadotrophin (β-HCG and age of the patient for the successful outcome in IVF. Materials and Methods: A retrospective study was done in 139 pregnancies after IVF at single IVF center from June 2007 to July 2012. The age of the patient and initial serum values of β-HCG on day 14 of embryo transfer were correlated with ongoing pregnancy (>12 weeks gestation. Results: The β-HCG level on day 14 of more than 347 mIU/ml has a sensitivity of 72.2% and specificity of 73.6% in prediction of pregnancy beyond 12 weeks period of gestation. Positive likelihood ratio (LR is 2.74 and negative LR is 0.37, (receiver operating characteristic area = 0.79. Discussion: In IVF cycles, there is a lot of stress on the couples while the cycle is going on. There was a positive correlation between the higher values of early serum β-HCG levels and ongoing pregnancy. Hence, it can be used as an independent predictor of a successful outcome of IVF cycle. Conclusion: We concluded from our study that early serum β-HCG can be used as a predictor of a successful outcome in IVF.

  1. Human chorionic gonadotropin administration is associated with high pregnancy rates during ovarian stimulation and timed intercourse or intrauterine insemination

    Directory of Open Access Journals (Sweden)

    Abdel-Razeq Sonya

    2004-07-01

    Full Text Available Abstract Background There are different factors that influence treatment outcome after ovarian stimulation and timed-intercourse or intrauterine insemination (IUI. After patient age, it has been suggested that timing of insemination in relation to ovulation is probably the most important variable affecting the success of treatment. The objective of this study is to study the value of human chorionic gonadotropin (hCG administration and occurrence of luteinizing hormone (LH surge in timing insemination on the treatment outcome after follicular monitoring with timed-intercourse or intrauterine insemination, with or without ovarian stimulation. Methods Retrospective analysis of 2000 consecutive completed treatment cycles (637 timed-intercourse and 1363 intrauterine insemination cycles. Stimulation protocols included clomiphene alone or with FSH injection, letrozole (an aromatase inhibitor alone or with FSH, and FSH alone. LH-surge was defined as an increase in LH level ≥200% over mean of preceding two days. When given, hCG was administered at a dose of 10,000 IU. The main outcome was clinical pregnancy rate per cycle. Results Higher pregnancy rates occurred in cycles in which hCG was given. Occurrence of an LH-surge was associated with a higher pregnancy rate with clomiphene treatment, but a lower pregnancy rate with FSH treatment. Conclusions hCG administration is associated with a favorable outcome during ovarian stimulation. Awaiting occurrence of LH-surge is associated with a better outcome with CC but not with FSH treatment.

  2. Mutations in a novel, cryptic exon of the luteinizing hormone/chorionic gonadotropin receptor gene cause male pseudohermaphroditism.

    Directory of Open Access Journals (Sweden)

    Nina Kossack

    2008-04-01

    Full Text Available BACKGROUND: Male pseudohermaphroditism, or Leydig cell hypoplasia (LCH, is an autosomal recessive disorder in individuals with a 46,XY karyotype, characterized by a predominantly female phenotype, a blind-ending vagina, absence of breast development, primary amenorrhea, and the presence of testicular structures. It is caused by mutations in the luteinizing hormone/chorionic gonadotropin receptor gene (LHCGR, which impair either LH/CG binding or signal transduction. However, molecular analysis has revealed that the LHCGR is apparently normal in about 50% of patients with the full clinical phenotype of LCH. We therefore searched the LHCGR for novel genomic elements causative for LCH. METHODS AND FINDINGS: In the present study we have identified a novel, primate-specific bona fide exon (exon 6A within the LHCGR gene. It displays composite characteristics of an internal/terminal exon and possesses stop codons triggering nonsense-mediated mRNA decay (NMD in LHCGR. Transcripts including exon 6A are physiologically highly expressed in human testes and granulosa cells, and result in an intracellular, truncated LHCGR protein of 209 amino acids. We sequenced exon 6A in 16 patients with unexplained LCH and detected mutations in three patients. Functional studies revealed a dramatic increase in the expression of the mutated internal exon 6A transcripts, indicating aberrant NMD. These altered ratios of LHCGR transcripts result in the generation of predominantly nonfunctional LHCGR isoforms, thereby preventing proper expression and functioning. CONCLUSIONS: The identification and characterization of this novel exon not only identifies a new regulatory element within the genomic organization of LHCGR, but also points toward a complex network of receptor regulation, including events at the transcriptional level. These findings add to the molecular diagnostic tools for LCH and extend our understanding of the endocrine regulation of sexual differentiation.

  3. Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight.

    Directory of Open Access Journals (Sweden)

    Charalambos Demetriou

    Full Text Available CONTEXT: Fetal growth involves highly complex molecular pathways. IGF2 is a key paternally expressed growth hormone that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been studied in term tissues. Conversely the maternally expressed growth suppressor, PHLDA2, has a significant negative correlation between its term placental expression and birth weight. OBJECTIVE: The aim of this study is to address the role in early gestation of expression of IGF1, IGF2, their receptors IGF1R and IGF2R, and PHLDA2 on term birth weight. DESIGN: Real-time quantitative PCR was used to investigate mRNA expression of IGF1, IGF2, IGF1R, IGF2R and PHLDA2 in chorionic villus samples (CVS (n = 260 collected at 11-13 weeks' gestation. Expression was correlated with term birth weight using statistical package R including correction for several confounding factors. RESULTS: Transcript levels of IGF2 and IGF2R revealed a significant positive correlation with birth weight (0.009 and 0.04, respectively. No effect was observed for IGF1, IGF1R or PHLDA2 and birth weight. Critically, small for gestational age (SGA neonates had significantly lower IGF2 levels than appropriate for gestational age neonates (p = 3.6 × 10(-7. INTERPRETATION: Our findings show that IGF2 mRNA levels at 12 weeks gestation could provide a useful predictor of future fetal growth to term, potentially predicting SGA babies. SGA babies are known to be at a higher risk for type 2 diabetes. This research reveals an imprinted, parentally driven rheostat for in utero growth.

  4. Human chorionic gonadotrophin in early gestation induces growth of estrogenic ovarian follicles and improves primiparous sow fertility during summer.

    Science.gov (United States)

    Seyfang, Jemma; Langendijk, P; Chen, T Y; Bouwman, E; Kirkwood, R N

    2016-09-01

    Reduced summer farrowing rates may be due to inadequate corpora luteal (CL) support. Porcine CL become dependent on LH from 12 d of pregnancy and the embryonic estrogen signal for maternal recognition of pregnancy (MRP) is initiated at about 11-12 d after insemination. We hypothesised that injection of the LH analogue human chorionic gonadotropin (hCG) would induce growth of estrogenic follicles and, by mimicking the signal for MRP and stimulating progesterone secretion, increase primiparous sow fertility. In Experiment 1, during a 28 d lactation 53 mixed parity sows were full-fed either throughout lactation (n=16) or until 18 d and then feed restricted during the last 10 d of lactation (n=36). At 12 d after mating restrict-fed sows were injected with 1000IU hCG (n=17) or were not injected (n=19); the full-fed sows acted as non-treated positive controls. Transrectal ovarian ultrasound exams were performed on days 12, 16, 20, 24, and 28; blood samples were obtained on days 12, 14, and 15 for estradiol and progesterone assay. For Experiment 2, during the summer months primiparous sows received 1000IU hCG 12 d after mating (n=28) or were non-injected controls (n=27). Pregnancy status was determined at 28 d and sows allowed to go to term to determine farrowing rates and litter sizes. In Experiment 1, injection of hCG increased (Pfeeding level on wean-estrus interval, farrowing rate or subsequent litter size. In Experiment 2, hCG injection was associated with a higher pregnancy rate (Psow fertility during the summer months.

  5. Energy status and HIF signalling in chorionic villi show no evidence of hypoxic stress during human early placental development.

    Science.gov (United States)

    Cindrova-Davies, T; van Patot, M Tissot; Gardner, L; Jauniaux, E; Burton, G J; Charnock-Jones, D S

    2015-03-01

    Early human placental and embryonic development occurs in a physiologically low oxygen environment supported by histiotrophic secretions from endometrial glands. In this study, we compare the placental metabolomic profile in the first, second and third trimesters to determine whether the energy demands are adequately met in the first trimester. We investigated whether hypoxia-inducible factors, HIF-1α and/or HIF-2α, might regulate transcription during the first trimester. First and second trimester tissue was collected using a chorionic villus sampling-like (CVS) technique. Part of each villus sample was frozen immediately and the remainder cultured under 2 or 21% O2 ± 1 mM H2O2, and ±the p38 MAPK pathway inhibitor, PD169316. Levels of HIF-1α were assessed by western blotting and VEGFA, PlGF and GLUT3 transcripts were quantified by RT-PCR. Term samples were collected from normal elective Caesarean deliveries. There were no significant differences in concentrations of ADP, NAD(+), lactate, and glucose, and in the ATP/ADP ratio, across gestational age. Neither HIF-1α nor HIF-2α could be detected in time-zero CVS samples. However, culture under any condition (2 or 21% O2 ± 1 mM H2O2) increased HIF-1α and HIF-2α. HIF-1α and HIF-2α were additionally detected in specimens retrieved after curettage. HIF-1α stabilization was accompanied by significant increases in VEGFA and GLUT3 and a decrease in PlGF mRNAs. These effects were suppressed by PD169316. In conclusion, our data suggest that first trimester placental tissues are not energetically compromised, and that HIF-1α is unlikely to play an appreciable role in regulating transcriptional activity under steady-state conditions in vivo. However, the pathway may be activated by stress conditions.

  6. Application of trans-abdominal chorionic villus sampling in prenatal diagnosis of chromosomal diseases in first trimester of gestation

    Institute of Scientific and Technical Information of China (English)

    Qi Qing-wei; Xiang Yang; Hao Na; Zhou Jing; Lu Ke; Tan Li; Sun Nian-hu

    2008-01-01

    Objective:To evaluate the feasibility and safety of prenatal diagnosis by traneabdominal chorionic villus sam-pling(TA-CVS)via the guidance of B-mode ultrasound in the first trimester of gestation.To explore the technique of long time culture and chromosome preparation of villi in early pregnancy.To evaluate the feasibility of the above techniques in the application of the prenatal cytogenetic diagnosis.Methods:One hundred and thirty-five singleton pregnancies at risk were referred from January 2001 to Decem-ber 2007.Results:The average maternal age was 35.2 years.TA-CVS was performed in the 10~13th weeks of gestation and the average gestational age was 10.89 weeks.All attempts at sampling were successful.The rate of operation-associated fetal loss was 0.74%.The failure rate of prenatal diagnosis because of inadequate amount of specimen was 0.The average culture time was 5-7 days.The success rate of the cell culture was 98.5%.No maternal con-temination and bacterial contamination happened.Fifteen cases of abnormal karyotype and one case of confined pla-cantel mosaiciem were diagnosed.Conclusion:TA-CVS appears to be safe and feasible and might to be offered in the prenatal diagnosis in the first trimester of gestation.The technique of long time culture and chromosome preparation of villi is stable and reliable.It is feasible to apply these techniques in the clinical practice of prenatal cytogenetic diagnose in the early pregnancy.

  7. Comparison of Pregnancy Rate in Simultaneous Human Chorionic Gonadotropin Administration with Intrauterine Insemination Vs. Standard intrauterine Insemination

    Directory of Open Access Journals (Sweden)

    Sahar Molaee

    2014-04-01

    Full Text Available Objective: Artificial insemination (AI has been used to treat infertile couples for approximately 200 years. Time interval between administration of Human Chorionic Gonadotropin (HCG and intrauterine insemination (IUI is one of controversial subjects in AI. Fertility rate is in the highest level on the day before ovulation. The aim of study is to compare pregnancy rate in standard IUI versus simultaneous HCG administration with IUI. Materials and Methods: In a randomized, controlled clinical trial, 141 women 20-35 years selected for IUI among patients referring to infertility clinic of Al-Zahra hospital in Tabriz, Iran in 2013.they were entered into study in two groups of simultaneous (n= 71 and standard (n = 70. After ovulation stimulation by clomiphene and human menopausal gonadotropin (HMG and creating up to 3 dominant follicles in each patient, a single dose of HCG was administered; thereafter, IUI was done in the simultaneous group instantly and 34-40 hours following HCG injection in the standard group. Results: In the standard and simultaneous groups, mean pregnancy rate per patient was 25.7% and 21.1% respectively (p= 0.52 and pregnancy rate per IUI cycle was 16.4% and 15.3% respectively (p= 0.82 that was not significantly different. Furthermore, between two groups in terms of age, the number of mature follicles, and endometrial thickness, there was no significant difference. Conclusion: Although difference in pregnancy rate between two groups was not statistically significant, simultaneous HCG with IUI is a comfort method for patients due to less cost and fewer patient's referrals to clinic.

  8. A unique case of growth hormone and human chorionic gonadotropin treatment in a 45,X male with Y: autosome translocation and literature review.

    Science.gov (United States)

    Mareri, Arianna; Iezzi, MariaLaura; Salvatore, Alessia; Ligas, Claudio; D'Alessandro, Elvira

    2016-07-01

    Maleness associated with a 45,X karyotype is a rare condition in childhood. It is usually diagnosed in adult age because of infertility. We report a unique case of an unbalanced translocation t(Y;21) in a 14-year-old boy with 45,X karyotype referred because of short stature, thin habitus and puberty delay. Hormone analysis showed low serum levels of basal testosterone, insulin-like growth factor (IGF-I) and gonadotrophins. Diagnosis of GH deficiency and puberty delay were made. He was treated with human chorionic gonadotropin (hCG) and GH therapy, respectively, for 6 and 24 months. PMID:27054600

  9. Human chorionic gonadotrophin in early gestation induces growth of estrogenic ovarian follicles and improves primiparous sow fertility during summer.

    Science.gov (United States)

    Seyfang, Jemma; Langendijk, P; Chen, T Y; Bouwman, E; Kirkwood, R N

    2016-09-01

    Reduced summer farrowing rates may be due to inadequate corpora luteal (CL) support. Porcine CL become dependent on LH from 12 d of pregnancy and the embryonic estrogen signal for maternal recognition of pregnancy (MRP) is initiated at about 11-12 d after insemination. We hypothesised that injection of the LH analogue human chorionic gonadotropin (hCG) would induce growth of estrogenic follicles and, by mimicking the signal for MRP and stimulating progesterone secretion, increase primiparous sow fertility. In Experiment 1, during a 28 d lactation 53 mixed parity sows were full-fed either throughout lactation (n=16) or until 18 d and then feed restricted during the last 10 d of lactation (n=36). At 12 d after mating restrict-fed sows were injected with 1000IU hCG (n=17) or were not injected (n=19); the full-fed sows acted as non-treated positive controls. Transrectal ovarian ultrasound exams were performed on days 12, 16, 20, 24, and 28; blood samples were obtained on days 12, 14, and 15 for estradiol and progesterone assay. For Experiment 2, during the summer months primiparous sows received 1000IU hCG 12 d after mating (n=28) or were non-injected controls (n=27). Pregnancy status was determined at 28 d and sows allowed to go to term to determine farrowing rates and litter sizes. In Experiment 1, injection of hCG increased (P<0.001) follicle diameter and serum concentrations of estradiol (P<0.01) and progesterone (P<0.05). There were no effects of lactation feeding level on wean-estrus interval, farrowing rate or subsequent litter size. In Experiment 2, hCG injection was associated with a higher pregnancy rate (P<0.05) and farrowing rate (P<0.08). There was no effect on litter size. These data confirm that hCG stimulates growth of estrogenic follicles and CL function, and improves primiparous sow fertility during the summer months. PMID:27397793

  10. Ovarian and Adrenal Androgens and Their Link to High Human Chorionic Gonadotropin Levels: A Prospective Controlled Study

    Directory of Open Access Journals (Sweden)

    René Rodríguez-Gutiérrez

    2014-01-01

    Full Text Available Background. Although the association between human chorionic gonadotropin (hCG and hyperandrogenism was identified more than 40 years ago, relevant questions remain unanswered. Design and Methods. We conducted a prospective, longitudinal, and controlled study in 23 women with a diagnosis of a complete hydatidiform mole (HM. Results. All participants completed the study. Before HM evacuation mean hCG was markedly higher in the cases than in the control group (P≤0.001. Free testosterone (T and dehydroepiandrosterone sulfate (DHEA-S were found to be higher in the cases (2.78 ± 1.24 pg/mL and 231.50 ± 127.20 μ/dL when compared to the control group (1.50 ± 0.75 pg/mL and 133.59 ± 60.69 μ/dL (P=0.0001 and 0.001, respectively. There was a strong correlation between hCG and free T/total T/DHEA-S concentrations (r=0.78; P≤0.001, r=0.74;  P≤0.001, and r=0.71;  P≤0.001, respectively. In the cases group 48 hours after HM evacuation, hCG levels were found to be significantly lower when compared to initial levels (P=0.001 and free T and DHEA-S declined significantly (P=0.0002 and 0.009. Conclusion. Before uterus evacuation, hCG, free T, and DHEA-S levels were significantly higher when compared with controls finding a strong correlation between hCG and free T/DHEA-S levels. Forty-eight hours after HM treatment hCG levels declined and the difference was lost. A novel finding of our study is that in cases, besides free T, DHEA-S was also found to be significantly higher and both the ovaries and adrenal glands appear to be the sites of this androgen overproduction.

  11. Optimisation of an oviposition protocol employing human chorionic and pregnant mare serum gonadotropins in the Barred Frog Mixophyes fasciolatus (Myobatrachidae

    Directory of Open Access Journals (Sweden)

    Clulow John

    2012-08-01

    Full Text Available Abstract Background Protocols for the hormonal induction of ovulation and oviposition are essential tools for managing threatened amphibians with assisted reproduction, but responses vary greatly between species and even broad taxon groups. Consequently, it is necessary to assess effectiveness of such protocols in representative species when new taxa become targets for induction. The threatened genus Mixophyes (family Myobatrachidae has amongst the highest proportion of endangered species of all the Australian amphibians. This study developed and optimised the induction of oviposition in a non-threatened member of this taxon, the great barred frog (Mixophyes fasciolatus. Methods Gravid female M. fasciolatus were induced to oviposit on one or more occasions by administration of human chorionic gonadotropin (hCG with or without priming with pregnant mare serum gonadotropin (PMSG. Treatments involved variations in hormone doses and combinations (administered via injection into the dorsal lymph sacs, and timing of administration. Pituitary homogenates from an unrelated bufonid species (Rhinella marina were also examined with hCG. Results When injected alone, hCG (900 to 1400 IU induced oviposition. However, priming with two time dependent doses of PMSG (50 IU, 25 IU increased responses, with lower doses of hCG (200 IU. Priming increased response rates in females from around 30% (hCG alone to more than 50% (p = 0.035, and up to 67%. Increasing the interval between the first PMSG dose and first hCG dose from 3 to 6 days also produced significant improvement (p Conclusions This study found that M. fasciolatus is amongst the few amphibian species (including Xenopus (Silurana and some bufonids that respond well to the induction of ovulation utilising mammalian gonadotropins (hCG. The optimal protocol for M. fasciolatus involved two priming doses of PMSG (50 IU and 25 IU administered at 6 and 4 days respectively, prior to two doses of hCG (100 IU, 24

  12. Follicle-stimulating hormone to substitute equine chorionic gonadotropin in the synchronization of ovulation in Santa Inês ewes

    Directory of Open Access Journals (Sweden)

    Bianor Matias Cardoso Neto

    2012-03-01

    Full Text Available The substitution of equine chorionic gonadotropin (eCG by follicle-stimulating hormone (FSH in protocols for synchronization of ovulation in Santa Inês ewes was assessed. Ten females were submitted to the insertion of intravaginal sponges containing 60 mg medroxyprogesterone acetate for 10 days; after this period sponges were withdrawn and the animals were randomly divided into two groups. Group 1 (n = 5: intramuscular injection of 0.5 mL d-cloprostenol and 300 UI eCG; Group 2 (n = 5: intramuscular injection of 0.5 mL d-cloprostenol and 20 mg FSH. Interval between sponge withdrawal and estrus beginning was 27.7 h and 35.9 h for eCG and FSH, respectively. Interval between sponge withdrawal and the end of estrus was 55.8 h for eCG treatment and 55.6 h for FSH treatment. Estrus length was 29.3 h and 19.6 h, for eCG and FSH treatments, respectively. The biggest follicle and the second in size measured 0.74 cm and 0.54 cm for eCG treatment, whereas for the FSH treatment they measured 0.73 and 0.50 cm. The interval between the beginning of estrus and ovulation was similar within all groups: 21.0 h for eCG treated ewes and 25.2 h for the ones treated with FSH. Ewes treated with eCG presented an interval of 47.5 h between sponge withdrawal and ovulation, while the ones treated with FSH presented a 61.1 h interval. Ovulation occurred 8.3 h before the end of estrus in the eCG group. On the other hand, ewes treated with FSH ovulated 5.5 h after the end of estrus. Estrus and ovulation were efficiently synchronized in Santa Inês ewes by using long-term progestogen protocol associated to the administration of 20 mg FSH, along with Prostaglandin F2α (PGF2α at the moment of sponge withdrawal, thus substituting the use of eCG.

  13. Low-dose human chorionic gonadotropin adjunct to an antagonist protocol in assisted reproductive technology: a randomized trial study.

    Directory of Open Access Journals (Sweden)

    Marzieh Aghahosseini

    2014-06-01

    Full Text Available The aim of this study was to evaluate the outcomes of adding low-dose hCG (human chorionic gonadotropin, as an LH active supplement, to a GnRH antagonist protocol in patients undergoing assisted reproduction techniques. In this parallel-group randomized clinical trial, 137 infertile female outpatients aged 20 - 39 years were randomized into two groups: hCG group and non-hCG group. All patients received r-FSH (150-300 IU and then a GnRH-antagonist, Cetrorelix (0.25 mg/day. Concomitantly with Cetrorelix, patients in the hCG group received low-dose hCG (200 IU daily, but the patients in the non-hCG group did not. 10,000 IU Urinary hCG (10,000 IU was injected to all patients, and ICSI was performed after oocyte retrieval. The primary outcome of this study was comparing the pregnancy rates between two study groups. Other differences between two groups such as serum estradiol concentration, fertilization rate, etc. were considered as secondary outcomes. A total of 130 patients completed this trial. No significant difference was detected between pregnancy rates of the two groups (P=0.52 as well as the fertilization, implantation and ongoing pregnancy rates (P=0.11, P=0.75 and P=0.06 respectively. The only significant difference between two groups was a higher concentration of estradiol in the hCG-treated patients (P<0.05. HCG-treated patients experienced a shorter treatment duration and a lower r-FSH required dose than the non-hCG group, but none of these differences were statistically significant (P=0.19 and P=0.10, respectively. The findings of the current study did not support advantages of adding low-dose hCG to GnRH antagonist plus r-FSH protocol in an unselected population of patients. Well-designed trials with a larger sample size for specific patients' subgroups are warranted.

  14. First-trimester maternal serum human chorionic gonadotrophin as a marker for fetal chromosomal disorders. The Dutch Working Party on Prenatal Diagnosis.

    Science.gov (United States)

    Van Lith, J M

    1992-06-01

    The Dutch Working Party on Prenatal Diagnosis has initiated a study on the possibilities of first-trimester screening for fetal chromosomal disorders. We report on maternal serum human chorionic gonadotrophin (MS-hCG) measurements in 1348 pregnancies with a chromosomally normal fetus and 53 pregnancies with a chromosomally abnormal fetus. The median MS-hCG concentration in 24 pregnancies with Down's syndrome was 1.19 multiples of the normal median (MoM). The MS-hCG distributions in normal and Down's syndrome pregnancies did not differ significantly (t-test: t = 1.945, p greater than 0.05). We also found no difference between normal pregnancies and pregnancies with other chromosomal disorders (six cases of trisomy 18, MoM = 0.80; four cases of sex chromosome abnormality, MoM = 1.01; 17 cases of chromosomal mosaicism in chorionic villi, MoM = 1.11). Selecting an upper limit at the 90th centile could detect 25 per cent of pregnancies with Down's syndrome. We conclude that, in the first trimester, MS-hCG as a screening factor for Down's syndrome is of minor value. However, MS-hCG could be a useful factor in a first-trimester screening programme based on a combination of markers. PMID:1387477

  15. Chorionic villus sampling

    Science.gov (United States)

    ... many different genetic conditions, including: Down syndrome Hemoglobinopathies Tay-Sachs disease Talk to your health care provider about ... chap 11. Read More Amniocentesis Biopsy Rh incompatibility Tay-Sachs disease Update Date 11/16/2014 Updated by: ...

  16. Chorionic Villus Sampling (CVS)

    Science.gov (United States)

    ... report card Careers Archives Pregnancy Before or between pregnancies Nutrition, weight & fitness Prenatal care Body & lifestyle changes Is it safe? ... Last reviewed: August, 2011 Pregnancy Before or between pregnancies Nutrition, weight & fitness Prenatal care Body & lifestyle changes Is it safe? ...

  17. A randomized three-way cross-over study in healthy pituitary-suppressed women to compare the bioavailability of human chorionic gonadotrophin (Pregnyl®) after intramuscular and subcutaneous administration

    NARCIS (Netherlands)

    Mannaerts, B.M.J.L.; Geurts, T.B.P.; Odink, J.

    1998-01-01

    The objective of this study was to compare the bioavailability of s.c. and i.m. administration of human chorionic gonadotrophin (HCG; Pregnyl®). In a randomized, single centre, three-way cross-over study, 18 healthy pituitary-suppressed volunteers were assigned to single HCG injections of 5000 and 1

  18. Human chorionic gonadotropin (hCG) regression normograms for patients with high-risk gestational trophoblastic neoplasia treated with EMA/CO (etoposide, methotrexate, actinomycin D, cyclophosphamide and vincristine) chemotherapy

    NARCIS (Netherlands)

    Lybol, C.; Westerdijk, K.; Sweep, F.C.; Ottevanger, P.B.; Massuger, L.F.A.G.; Thomas, C.M.G.

    2012-01-01

    Background We present normograms for human chorionic gonadotropin (hCG) regression in patients with high-risk gestational trophoblastic neoplasia (GTN) successfully treated with multiagent chemotherapy in order to predict treatment resistance. Patients and methods We collected data for 46 patients w

  19. Birth after human chorionic gonadotropin-primed oocyte in vitro maturation and fertilization with testicular sperm in a normo-ovulatory patient

    Science.gov (United States)

    González-Ortega, Claudia; Piña-Aguilar, Raul Eduardo; Cancino-Villareal, Patricia; Gutiérrez-Gutiérrez, Antonio Martin

    2016-01-01

    In this report, we present a case of in vitro maturation (IVM) with surgical retrieved testicular sperm in a normo-ovulatory female. Human chorionic gonadotropin-primed IVM, testicular biopsy for sperm retrieval and intracytoplasmic sperm injection with fresh sperm were performed. Fourteen cumulus-oocyte complexes were obtained in germinal vesicle or metaphase I stage, eight oocytes reached metaphase II, seven presumptive zygotes were obtained, and three cleavage stages embryos in day 2 were transferred producing a singleton pregnancy. A single healthy newborn was obtained. Our results suggest that IVM may be an alternative for in vitro fertilization in normo-ovulatory women even if surgical retrieval of sperm is needed. Further research is required to depict contributing factors to the success of IVM in indications different from polycystic ovaries syndrome and the role of male gamete. PMID:27803591

  20. Endometrial cysteine-rich secretory protein 3 is inhibited by human chorionic gonadotrophin, and is increased in the decidua of tubal ectopic pregnancy

    DEFF Research Database (Denmark)

    Horne, A W; Duncan, W C; King, A E;

    2009-01-01

    gestation-matched women undergoing surgical termination of pregnancy (n = 8), evacuation of uterus for miscarriage (n = 6) and surgery for EP (n = 11) was subjected to quantitative RT-PCR, morphological assessment, immunohistochemistry and western blot analysis. Sera were analysed for progesterone and human...... endometrium from women with ectopic and intrauterine gestations could be used to identify candidate diagnostic biomarkers for EP. The aim of this study was to further investigate the decidual gene with the highest fold increase in EP, cysteine-rich secretory protein-3 (CRISP-3). Decidualized endometrium from...... chorionic gonadotrophin (hCG) levels. Immortalized endometrial epithelial cells were cultured with physiological concentrations of hCG. CRISP-3 mRNA and protein expression were greater in endometrium from ectopic when compared with intrauterine pregnancies (P protein was localized...

  1. 44 cases of transabdominal chorionic villus biopsy applied analysis%44例经腹绒毛活检产前诊断的应用分析

    Institute of Scientific and Technical Information of China (English)

    邓璐莎; 郭晓玲; 钟进; 陈建仪; 朱晓丹; 伍秋艳

    2013-01-01

    XObjective: Analysize the transabdominal chorionic villus biopsy in prenatal diagnosis. Methods: Selected 44 cases with single fetus in 2010 January to 2011 December in our hospital out - patient examination with the indications for prenatal diagnosis of informed consent by transabdominal chorionic villus biopsy, and selected the same period with the indications for prenatal diagnosis of umbilical vein puncture line of singleton pregnancies in 50 cases were compared, respectively to calculate its operation success rate and pregnancy loss rate. Results; 44 cases of abdominal cashmere wool biopsy in pregnant women, check out the chromosomal abnormality in 4 cases, a case of trisomy 18,2 cases of trisomy 21, 1 cases with 45, XO, 5 cases were severe a - thalassemia, induction of labor. Among it, 1 patient with fetal death in utero after transabdominal chorionic villus in 3 days, which of chromosome diagnosed trisomy 21. The remaining track until birth, without placenta! hematoma, placenta] abruption, vaginal bleeding, fetal structural abnormalities, operation success rate of 100% , pregnancy loss rate of 2. 27% , compared to abdominal umbilical vein puncture, P > 0. 05, the difference was not statistically significant, can not think of two methods of operation success rate and pregnancy loss rate unequal. But the umbilical vein puncture in general need after 22 weeks of pregnancy, the transabdominal chorionic villus biopsy in early and timely discovery , pregnant women to reduce the emotional and psychological pressure. Conclusion: Early pregnant chorionic villi biopsy with early diagnosis, high accuracy, less complications, is a safe, accurate and reliable prenatal diagnostic techniques.%目的 对经腹绒毛活检在产前诊断中的应用分析.方法 选择2010年1月至2011年12月在我院产检门诊就诊有产前诊断指征的单胎孕妇44例,经知情同意后行经腹绒毛活检,同时选择同时期有产前诊断指征的行

  2. Independent prognostic value of preoperative serum markers CA 242, specific tissue polypeptide antigen and human chorionic gonadotrophin beta, but not of carcinoembryonic antigen or tissue polypeptide antigen in colorectal cancer.

    OpenAIRE

    Carpelan-Holmström, M; Haglund, C.; Lundin, J; Alfthan, H.; Stenman, U H; Roberts, P. J.

    1996-01-01

    The prognostic value of preoperative serum concentrations of carcinoembryonic antigen (CEA), CA 242, tissue polypeptide antigen (TPA), specific tissue polypeptide antigen (TPS) and human chorionic gonadotrophin beta (hCG beta) in 251 patients with colorectal cancer (39 Dukes' A, 98 Dukes' B, 56 Dukes' C and 58 Dukes' D) was investigated. When using the cut-off levels recommended for diagnostic purposes, there was a significantly longer overall survival in patients with low tumour marker level...

  3. The Analysis of Early Pregnancy Human Chorionic Gonadotropin Level%孕早期人绒毛膜促性腺激素水平分析

    Institute of Scientific and Technical Information of China (English)

    徐丽

    2015-01-01

    目的:分析孕早期人绒毛膜促性腺激素水平。方法将我院进行人绒毛膜促性腺激素检查的678例孕早期女性按照病史、妇科检查、B超检查结果分为正常妊娠组,先兆流产组,胚停组,异位妊娠组,然后对所有孕早期女性进行β-HCG水平测定。结果四组第六周的β-HCG均高于第五周,呈逐渐上升的趋势。先兆流产组、胚停组和异位妊娠组第五、第六周β-HCG 值及48 h倍增率均低于同期正常妊娠组,差异有统计学意义(P<0.05)。结论孕早期血清HCG水平检测对于先兆流产、异位妊娠等的诊断、鉴别诊断、转归、预后,避免过度治疗或过分保胎形成过期流产继发凝血功能障碍有重要的临床意义。%Objective Analysis early pregnancy human chorionic gonadotropin level.Methods678 cases of early pregnancy human chorionic gonadotropin in our hospital were divided into normal pregnancy, threatened abortion, embryo stop the group, ectopic pregnancy group according to history, gynecological examination, B-ultrasound, all early pregnant women were measured early β-HCG level.ResultsFour groups of six weeks of β-HCG were significantly higher than the fifth week, showed an increasing trend. Threatened abortion group, the embryos stop group and ectopic pregnancy group iffth and sixth weeks of β-HCG value and 48 h were significantly lower than doubled over the same period in normal pregnancy group, the difference was statistically signiifcant (P<0.05). Conclusion It has important clinical significance for Trimester serum HCG level detection for threatened abortion, ectopic pregnancy, such as diagnosis, differential diagnosis, prognosis, prognosis, can avoid over-treatment or excessive formation of missed abortion miscarriage secondary coagulopathy.

  4. Contribution of aquaporin 9 and multidrug resistance-associated protein 2 to differential sensitivity to arsenite between primary cultured chorion and amnion cells prepared from human fetal membranes

    Energy Technology Data Exchange (ETDEWEB)

    Yoshino, Yuta [Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392 (Japan); Yuan, Bo, E-mail: yuanbo@toyaku.ac.jp [Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392 (Japan); Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, 1550 4th St, RH584E Box 2911 San Francisco, CA 94158-2911 (United States); Kaise, Toshikazu [Laboratory of Environmental Chemodynamics, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392 (Japan); Takeichi, Makoto [Yoneyama Maternity Hospital, 2-12 Shin-machi, Hachioji, Tokyo 192-0065 (Japan); Tanaka, Sachiko; Hirano, Toshihiko [Department of Clinical Pharmacology, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392 (Japan); Kroetz, Deanna L. [Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, 1550 4th St, RH584E Box 2911 San Francisco, CA 94158-2911 (United States); Toyoda, Hiroo [Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392 (Japan)

    2011-12-15

    Arsenic trioxide (arsenite, As{sup III}) has shown a remarkable clinical efficacy, whereas its side effects are still a serious concern. Therefore, it is critical to understand the effects of As{sup III} on human-derived normal cells for revealing the mechanisms underlying these side effects. We examined the effects of As{sup III} on primary cultured chorion (C) and amnion (A) cells prepared from human fetal membranes. A significant dose-dependent As{sup III}-mediated cytotoxicity was observed in the C-cells accompanied with an increase of lactate dehydrogenase (LDH) release. Higher concentrations of As{sup III} were required for the A-cells to show cytotoxicity and LDH release, suggesting that the C-cells were more sensitive to As{sup III} than the A-cells. The expression levels of aquaporin 9 (AQP9) were approximately 2 times higher in the C-cells than those in the A-cells. Both intracellular arsenic accumulation and its cytotoxicity in the C-cells were significantly abrogated by sorbitol, a competitive AQP9 inhibitor, in a dose-dependent manner. The protein expression levels of multidrug resistance-associated protein (MRP) 2 were downregulated by As{sup III} in the C-cells, but not in the A-cells. No significant differences in the expression levels of MRP1 were observed between C- and A-cells. The protein expression of P-glycoprotein (P-gp) was hardly detected in both cells, although a detectable amount of its mRNA was observed. Cyclosporine A, a broad-spectrum inhibitor for ABC transporters, and MK571, a MRP inhibitor, but not PGP-4008, a P-gp specific inhibitor, potently sensitized both cells to As{sup III}-mediated cytotoxicity. These results suggest that AQP9 and MRP2 are involved in controlling arsenic accumulation in these normal cells, which then contribute to differential sensitivity to As{sup III} cytotoxicity between these cells. -- Highlights: Black-Right-Pointing-Pointer Examination of effect of As{sup III} on primary cultured chorion (C) and amnion

  5. The value of placental protein 13, β-human chorionic gonadotropin and progesterone in the prediction of miscarriages in threatened miscarriage patients.

    Science.gov (United States)

    Yalçin, I; Taşkin, S; Pabuçcu, E G; Söylemez, F

    2015-04-01

    The aim of this paper was to investigate the levels of maternal serum placental protein13 (PP13), beta human chorionic gonadotropin (β-hCG) and progesterone in the prediction of miscarriages in threatened miscarriages. A total of 110 patients with a gestational age threatened miscarriage) and 68 patients were allocated as controls. A total of six miscarriages were observed in the study group. β-hCG levels were significantly lower in the group with threatened miscarriage when compared with controls (p = 0.018). There was no statistically significant difference in regard to progesterone and PP13 levels occurred between two groups (p = 0.653 and p = 0.062, respectively). Following receiver operating characteristic (ROC) analysis, the β-hCG parameter was found useful in differentiating miscarriages from the threatened miscarriage group (p = 0.031). PP13 and progesterone parameters in predicting miscarriages were not found as statistically significant (p = 0.084 and p = 0.914, respectively). This study suggests that β-hCG measurements could be useful in predicting spontaneous miscarriage in women presenting with threatened miscarriage. Even though PP13 seems unfeasible to be used as a predictive marker for miscarriage, factors affecting PP13 levels should be considered along with the need for comprehensive studies including larger patient populations. PMID:25153203

  6. Diagnostic sensitivity of serum carcinoembryonic antigen, carbohydrate antigen 19-9, alpha-fetoprotein, and beta-human chorionic gonadotropin in esophageal carcinoma (receiver operating characteristic curve analysis

    Directory of Open Access Journals (Sweden)

    Bhawna Bagaria

    2015-01-01

    Full Text Available Background: Esophageal carcinomas are very lethal disease relatively unresponsive to therapy. The continued development of new and more effective chemotherapeutic agents and regimens offers hope that in the future, this carcinoma may be amenable to either more effective palliative treatment or possibly increased cure. We, therefore, aimed to evaluate the marker with best diagnostic sensitivity in esophageal carcinoma. Materials and Methods: Serum carcinoembryonic antigen (CEA, carbohydrate antigen 19-9 (CA19-9, alpha-fetoprotein (AFP, and beta-human chorionic gonadotropin (β-HCG levels were assessed in healthy subjects (n = 50 and patients (n = 50 initially diagnosed of esophageal carcinoma by endoscopic examination and biopsy before receiving any therapy. The data were analyzed using SPSS software version 10.0 (SPSS Inc. USA and MedCalc to estimate mean ± standard deviation, the significance of the observed differences (P value, for calculating sensitivity and for plotting receiver operating characteristic curves. Results: Sensitivity of CEA, CA19-9, AFP, and β-HCG detected in esophagus cancer was 38%, 18%, 10%, and 26% respectively. Conclusion: From the above studied markers, CEA has the highest sensitivity followed by β-HCG, CA19-9 and AFP. Although the sensitivity of tumor markers in esophagus cancer is low, they may be useful additional parameter in the prediction of neoplasms involved at the early stage of tumor growth.

  7. Down-regulation of messenger ribonucleic acid encoding an importer of sulfoconjugated steroids during human chorionic gonadotropin-induced follicular luteinization in vivo.

    Science.gov (United States)

    Brown, Kristy A; Bouchard, Nadine; Lussier, Jacques G; Sirois, Jean

    2007-01-01

    Members of the organic anion transporting polypeptide (SLCO/OATP) superfamily are capable of importing anionic compounds across the lipid bilayer in a sodium-independent manner. Member 2B1 has been shown to transport few substrates, two of which are dihydroepiandrosterone-3-sulfate (DHEA-S) and estrone-3-sulfate. Steroid sulfatase (STS) catalyses the hydrolysis of these steroids into their unconjugated counterparts. The objective of this study was to investigate the regulation of SLCO2B1 and STS mRNAs during human chorionic gonadotropin (hCG)-induced ovulation/luteinization. The equine SLCO2B1 cDNA was cloned and shown to encode a 709-amino acid protein (OATP2B1) that is highly conserved when compared to mammalian orthologs. RT-PCR/Southern blot analyses were performed to study the regulation of SLCO2B1 and STS transcripts in equine preovulatory follicles isolated between 0 and 39h after hCG treatment. Results showed high levels of SLCO2B1 mRNA expression before hCG, with a marked decrease observed in follicles obtained 24-39h post-hCG (Pimport sulfoconjugated DHEA and estrogens, and that STS can remove the sulfonate moiety from these steroids, their down-regulation in luteinizing preovulatory follicles may provide an additional biochemical basis for the decrease in ovarian 17beta-estradiol biosynthesis after the LH surge.

  8. False Negative NIPT Results: Risk Figures for Chromosomes 13, 18 and 21 Based on Chorionic Villi Results in 5967 Cases and Literature Review.

    Directory of Open Access Journals (Sweden)

    Diane Van Opstal

    Full Text Available Non-invasive prenatal testing (NIPT demonstrated a small chance for a false negative result. Since the "fetal" DNA in maternal blood originates from the cytotrophoblast of chorionic villi (CV, some false negative results will have a biological origin. Based on our experience with cytogenetic studies of CV, we tried to estimate this risk. 5967 CV samples of pregnancies at high risk for common aneuplodies were cytogenetically investigated in our centre between January 2000 and December 2011. All cases of fetal trisomy 13, 18 and 21 were retrospectively studied for the presence of a normal karyotype or mosaicism < 30% in short-term cultured (STC- villi. 404 cases of trisomies 13, 18 and 21 were found amongst 5967 samples (6,8%. Of these 404 cases, 14 (3,7% had a normal or low mosaic karyotype in STC-villi and therefore would potentially be missed with NIPT. It involved 2% (5/242 of all trisomy 21 cases and 7.3% (9/123 of all trisomy 18 cases. In 1:426 (14/5967 NIPT samples of patients at high risk for common aneuploidies, a trisomy 18 or 21 will potentially be missed due to the biological phenomenon of absence of the chromosome aberration in the cytotrophoblast.

  9. Stereological and morphometric analysis of collagen and seminiferous tubules in testes of patients with cryptorchidism submitted or not to treatment with human chorionic gonadotrophin

    Directory of Open Access Journals (Sweden)

    Luciano A. Favorito

    2005-12-01

    Full Text Available OBJECTIVES: Quantify the distribution of collagen and analyze the seminiferous tubules diameter in the testis of patients with cryptorchidism, to verify if the previous use of human chorionic gonadotrophin (hCG affects these structures. MATERIALS AND METHODS: Samples of parenchymal tissue of cryptorchid testis obtained during peroperative biopsies were collected from 26 patients. Sixteen samples were embedded in paraffin and stained with picrosirius red to evidence fibers of collagen system. The quantification of these fibers was determined by stereological methods, using a test system M-42. To obtain seminiferous tubules diameter we used 10 of the 26 samples. These samples were embedded in Epon and the analyses were carried out in semi-thin sections, stained with toluidin blue. The selected results of each group were statistically analyzed and compared by the student's t and Tukey-Kramer's tests. RESULTS: The testicular interstitium and lamina propria of patients treated with hCG showed statistically significant less collagen system fibers, when compared to the testes of patients nontreated (0.30% versus 0.39%, p = 0.0079. The seminiferous tubules diameters were not statistically significant different between the testes of patients treated and nontreated with hCG (67.5 versus 59.35 µm, p = 0.0609. CONCLUSIONS: hCG use in the cryptorchidism could delay, at least temporarily, a progressive growth of fibers of collagen system. We did not find statistically significant difference in the seminiferous tubular diameters between treated and nontreated patients.

  10. Elevated levels of beta-human chorionic gonadotropin and human placental lactogen between 11-13 week's gestation and subsequent pregnancy complications in Oman i women

    International Nuclear Information System (INIS)

    The association between abnormal levels of maternal serum b-human chorionic gonadotrophin (b-HCG) and human placental lactogen (HPL) measured in early pregnancy and future poor pregnancy outcome is fairly well established. Little is known on how such prognostic information can be used to avoid future complications of pregnancy and improve their outcome. We undertook a prospective study, which was designed to assess the efficiency of maternal serum biochemical markers b-HCG and HPL for the detection of different pregnancy and labor complications. The blood samples were taken from pregnant patients who attended the antenatal clinic at Sultan Qaboos University Hospital in Muscat, Sultanate of Oman, from December 2001 to October 2002. These patients were selected randomly. A total of 200 Omani women, none diabetic, with singleton pregnancies between 11 and 13 weeks gestation were recruited into the study. Gestational age was calculated from the first day of the last menstrual period, unless ultrasonography showed a discrepancy of more than 14 days. Excluded from the study were pregnancies with fetal anomalies, multiple gestations and insulin dependent maternal diabetes. Biochemical analysis of both maternal serum markers was performed in the clinical biochemistry laboratory using an automated immunometric technique supplied by Beckman Coulter for b-HCG and manual radioimmunoassay utilizing a gamma radiation scintillation counter for HPL. Both assays underwent internal, trilevel quality controls. The selected patients were monitored for the following complications: pregnancy induced hypertension (PIH), gestational diabetes, polyhydramnios, antepartum hemorrhage, intrauterine growth retardation (IUGR) (birth weight <10th per centile for gestation), low Apgar score (7 or less) and emergency cesarean section. 75 patients did not develop any pregnancy complications and delivered vaginally. This was our control group. 85 patients developed one of the above mentioned problems

  11. A prospective, randomised comparative study of weekly versus biweekly application of dehydrated human amnion/chorion membrane allograft in the management of diabetic foot ulcers

    Science.gov (United States)

    Zelen, Charles M; Serena, Thomas E; Snyder, Robert J

    2014-01-01

    The aim of this study is to determine if weekly application of dehydrated human amnion/chorion membrane allograft reduce time to heal more effectively than biweekly application for treatment of diabetic foot ulcers. This was an institutional review board-approved, registered, prospective, randomised, comparative, non-blinded, single-centre clinical trial. Patients with non-infected ulcers of ≥ 4 weeks duration were included for the study. They were randomised to receive weekly or biweekly application of allograft in addition to a non-adherent, moist dressing with compressive wrapping. All wounds were offloaded. The primary study outcome was mean time to healing. Overall, during the 12-week study period, 92·5% (37/40) ulcers completely healed. Mean time to complete healing was 4·1 ± 2·9 versus 2·4 ± 1·8 weeks (P = 0·039) in the biweekly versus weekly groups, respectively. Complete healing occurred in 50% versus 90% by 4 weeks in the biweekly and weekly groups, respectively (P = 0·014). Number of grafts applied to healed wounds was similar at 2·4 ± 1·5 and 2·3 ± 1·8 for biweekly versus weekly groups, respectively (P = 0·841). These results validate previous studies showing that the allograft is an effective treatment for diabetic ulcers and show that wounds treated with weekly application heal more rapidly than with biweekly application. More rapid healing may decrease clinical operational costs and prevent long-term medical complications. PMID:24618401

  12. The Higher Response of Vascular Endothelial Growth Factor and Angiotensin-II to Human Chorionic Gonadotropin in Women with Polycystic Ovary Syndrome

    Directory of Open Access Journals (Sweden)

    Junwei Qu

    2015-02-01

    Full Text Available Background: This research investigated the response of vascular active factors, vascular endothelial growth factor (VEGF and angiotensin-II (AT-II to ovarian stimulation during 24 hours in patients with polycystic ovary syndrome (PCOS. Materials and Methods: In this clinical trial study, 52 patients with PCOS and 8 control cases were stimulated with human chorionic gonadotropin (HCG on the 4th to 7th day of the patients’ natural or induced menstrual cycles. We measured VEGF and AT-II by radioimmunoassay before the injection (0 hour and 3, 8, 12, 18 and 24 hours after the stimulation. Results: After ovarian stimulation, there was substantially higher level of VEGF in typical PCOS patients than the other three groups at the 3 hour time point (p<0.05, while there were no significant differences in VEGF at all the other time points among the four groups. As for AT-II, before and at all time points after the ovarian stimulation, it seemed that the AT-II levels in patients’ sera with different phenotypes of PCOS by the Rotterdam criteria were all higher than in the control group although the differences were not statistically significant. The level of AT-II in typical PCOS patients was also significantly higher than the other three groups at the 3 hour time point (p<0.05, while no significant differences at all the other time points among the four groups were observed. Conclusion: The response to the stimulation varied among patients with different phenotypes of PCOS according to the Rotterdam criteria. Serum VEGF and AT-II were possible contributors to an increased risk of developing ovarian hyperstimulation syndrome (OHSS in patients with typical PCOS during the early follicular phase (3 hours after ovarian stimulation (Registration Number: NCT02265861.

  13. Expression of VEGF receptors VEFGR-1 and VEGFR-2, angiopoietin receptors Tie-1 and Tie-2 in chorionic villi tree during early pregnancy.

    Directory of Open Access Journals (Sweden)

    Ramazan Demir

    2010-02-01

    Full Text Available The aim of this study was to determine the expression of VEGF and its receptors in placentas from normal pregnancies between 22 days p.c. and 48 days p.c. of very early pregnancy. Placental tissues carried out from 19 pregnant women were examined. Immunohistochemical technique, electron microscopy were employed to evaluate the factors expression. In the new developing mesenchymal villi and immature intermediate villi VEGF and its receptors VEGFR-1 and VEGFR-2 immunoreactivity was detected in all the placental components, while in the stem villi and in the chorionic plate with large vessels only in some components. In the mesenchymal villi and immature intermediate villi VEGFR-1 and -2, and angiopoietin receptors Tie-1 and -2 immunoreactivity was dominantly observed in the heamangiogenic cells and cells cords, whereas the matured villi showed immunoreactivity only in other components. The ultrastructural findings were higher in respect to the all of the early pregnancy days. The placental samples from all of pregnancies, showed the VEGF and its receptors in optimal expression levels, whereas the angiopoietin receptors Tie-1 and -2 showed a higher expression levels in respect to other study factors. The receptors protein levels increased from the early days to the advanced days of gestation, but this alteration was not significant. The intensity of the immunolabeling for these proteins were not significant compared to to each other of gestatin days were examined. These findings demonstrated that a dysregulation of the placental expression of the VEGF and its receptors related to the different degrees of the gestational periods. Probably, this event may be related to complete vasculugenesis and angiogenesis in placental villi.

  14. Contribution of aquaporin 9 and multidrug resistance-associated protein 2 to differential sensitivity to arsenite between primary cultured chorion and amnion cells prepared from human fetal membranes.

    Science.gov (United States)

    Yoshino, Yuta; Yuan, Bo; Kaise, Toshikazu; Takeichi, Makoto; Tanaka, Sachiko; Hirano, Toshihiko; Kroetz, Deanna L; Toyoda, Hiroo

    2011-12-01

    Arsenic trioxide (arsenite, As(III)) has shown a remarkable clinical efficacy, whereas its side effects are still a serious concern. Therefore, it is critical to understand the effects of As(III) on human-derived normal cells for revealing the mechanisms underlying these side effects. We examined the effects of As(III) on primary cultured chorion (C) and amnion (A) cells prepared from human fetal membranes. A significant dose-dependent As(III)-mediated cytotoxicity was observed in the C-cells accompanied with an increase of lactate dehydrogenase (LDH) release. Higher concentrations of As(III) were required for the A-cells to show cytotoxicity and LDH release, suggesting that the C-cells were more sensitive to As(III) than the A-cells. The expression levels of aquaporin 9 (AQP9) were approximately 2 times higher in the C-cells than those in the A-cells. Both intracellular arsenic accumulation and its cytotoxicity in the C-cells were significantly abrogated by sorbitol, a competitive AQP9 inhibitor, in a dose-dependent manner. The protein expression levels of multidrug resistance-associated protein (MRP) 2 were downregulated by As(III) in the C-cells, but not in the A-cells. No significant differences in the expression levels of MRP1 were observed between C- and A-cells. The protein expression of P-glycoprotein (P-gp) was hardly detected in both cells, although a detectable amount of its mRNA was observed. Cyclosporine A, a broad-spectrum inhibitor for ABC transporters, and MK571, a MRP inhibitor, but not PGP-4008, a P-gp specific inhibitor, potently sensitized both cells to As(III)-mediated cytotoxicity. These results suggest that AQP9 and MRP2 are involved in controlling arsenic accumulation in these normal cells, which then contribute to differential sensitivity to As(III) cytotoxicity between these cells.

  15. Preliminary evidence for associations between second-trimester human chorionic gonadotropin and unconjugated oestriol levels with pregnancy outcome in Down syndrome pregnancies.

    Science.gov (United States)

    Benn, P A

    1998-04-01

    Fifty-six cases of Down syndrome were identified in a population of women who had undergone maternal serum triple marker screening [alpha-fetoprotein (AFP), human chorionic gonadotropin (hCG), and unconjugated oestriol (uE3) analyses]. These affected pregnancies represented all known cases present in the population of 34,368 women screened. Using a 1:270 mid-trimester Down syndrome risk to define the screen-positive group, 42 affected pregnancies were screen-positive (medians: AFP = 0.79 MOM, hCG = 2.13 MOM, uE3 = 0.62 MOM, age 34.6 years) and 14 pregnancies were screen-negative (medians: AFP = 0.82 MOM, hCG = 1.57 MOM, uE3 = 0.92 MOM, age 24.2 years). Four affected pregnancies were associated with in utero death and each of these cases was associated with relatively extreme values of AFP, hCG, and uE3, including the three highest levels of hCG in the entire series of Down syndrome pregnancies. Twenty-nine (15 screen-positive and 14 screen-negative) affected pregnancies resulted in liveborns. Down syndrome pregnancies had a significantly shorter gestational term than controls, and Down syndrome babies were also lighter than controls, even after adjustment for sex and gestational age. In affected pregnancies, a low uE3 level appeared to be associated with a greater chance of a small-for-gestational age baby. No correlations could be demonstrated between AFP or hCG levels and gestational age-adjusted term weight. Based on this small series, it would appear that uE3 may be particularly useful in detecting those Down syndrome cases associated with small-for-gestational age fetuses. A very high hCG value may indicate a higher probability of fetal death. PMID:9602476

  16. Dose- and time-related changes in aerobic metabolism, chorionic disruption, and oxidative stress in embryonic medaka (Oryzias latipes): Underlying mechanisms for silver nanoparticle developmental toxicity

    International Nuclear Information System (INIS)

    Silver nanoparticles (AgNPs) are widely employed in commercial products, and are thus inevitably released into the aquatic environment. Many studies have indicated that AgNPs could induce toxicological effects on embryonic fish. To understand the mechanism of AgNP developmental toxicity, we determined the effects of AgNPs on the egg membrane, aerobic metabolism, antioxidant system, lipid peroxidation, as well as reactive oxygen species (ROS) and singlet oxygen (1O2) generation in early-life medaka fish (Oryzias latipes). AgNP treatment at 62.5–1000 μg/L caused significant increase in retarded development and abnormalities. Destruction of the surface ornamentation and egg envelope was observed at a higher AgNP concentration (≥125 μg/L) using light microscopy and scanning electron microscopy. A dose-dependent increase in lactate dehydrogenase activity, an indicator of anaerobic metabolism, and superoxide dismutase activity was observed in the treated embryos. In contrast, the total reduced glutathione level decreased. A high thiobarbituric acid reactive substance concentration was generated upon AgNP exposure from day 1 to day 7 postfertilisation. The biochemical parameters suggested that oxidative stress was induced by the AgNPs. Unexpectedly, a dose-dependent reduction in ROS and 1O2 generation upon high AgNP exposure (≥250 μg/L) was observed. Although the morphological damages induced by the AgNPs were irreversible, restorable antioxidant defenses were noted in the well-developed embryos. This finding supported the idea that the stage of morphogenesis and organogenesis is a critical window to chemical exposure or environmental stress. Overall, the results suggested that hypoxia, disturbed egg chorion, and oxidative stress are mechanistically associated with AgNP toxicity in embryonic fish.

  17. Reproductive outcomes of Alpine goats primed with progesterone and treated with human chorionic gonadotropin during the anestrus-to-estrus transition season.

    Science.gov (United States)

    Alvarado-Espino, A S; Meza-Herrera, C A; Carrillo, E; González-Álvarez, V H; Guillen-Muñoz, J M; Ángel-García, O; Mellado, M; Véliz-Deras, F G

    2016-04-01

    This study aimed to determine the possible effects of a single injection of human chorionic gonadotropin (hCG) as a means for estrus induction in acyclic French-Alpine goats during the reproductive transition period at 25°N, 103°W. The potential effects of hCG upon ovarian function and reproductive performance of goats were also assessed. Multiparous acyclic French-Alpine goats (n = 39; 37.4 ± 8 .5 kg) were primed with 20mg progesterone (P4) 1 day prior to hCG administration. Thereafter, does were treated either with saline (hCG-0; n = 10), 50 (hCG-50; n = 9), 100 (hCG-100; n = 10), or 300 IU of hCG (hCG-300; n = 10). Ovarian structures and pregnancy were monitored by transrectal ultrasonography. In addition, after hCG application, goats were monitored twice daily (0800 and 1800 h) to detect estrus signs, with the use of aproned, sexually active bucks treated with testosterone. Goats were bred 12h after the onset of estrus. Two days after hCG administration, the number of large follicles was higher (P 0.05), being always accompanied by ovulation. Pregnancy rate (67, 100, and 70%), kidding rate (55%, 80%, and 70%), and litter size (1.6 ± 0.5, 1.5 ± 0.5, and 1.5 ± 0.5) for hCG-50, hCG-100, and hCG-300, respectively, did not differ among the hCG-treated does. Therefore, the combined use of P4-priming plus a 100-IU hCG injection is an effective protocol for inducing estrus in non-cycling Alpine goats during the anestrus-to-estrus transition period, which is of key importance for both goat producers and industrializers. PMID:26944772

  18. A comparison of human chorionic gonadotropin and luteinizing hormone releasing hormone on the induction of spermiation and amplexus in the American toad (Anaxyrus americanus

    Directory of Open Access Journals (Sweden)

    Kouba Andrew J

    2012-08-01

    Full Text Available Abstract Background Captive breeding programs for endangered amphibian species often utilize exogenous hormones for species that are difficult to breed. The purpose of our study was to compare the efficacy of two different hormones at various concentrations on sperm production, quantity and quality over time in order to optimize assisted breeding. Methods Male American toads (Anaxyrus americanus were divided into three separate treatment groups, with animals in each group rotated through different concentrations of luteinizing hormone releasing hormone analog (LHRH; 0.1, 1.0, 4.0 and 32 micrograms/toad, human chorionic gonadotropin (hCG; 50, 100, 200, and 300 IU, or the control over 24 hours. We evaluated the number of males that respond by producing spermic urine, the sperm concentration, percent motility, and quality of forward progression. We also evaluated the effects of hCG and LHRH on reproductive behavior as assessed by amplexus. Data were analyzed using the Generalized Estimating Equations incorporating repeated measures over time and including the main effects of treatment and time, and the treatment by time interaction. Results The hormone hCG was significantly more effective at stimulating spermiation in male Anaxyrus americanus than LHRH and showed a dose-dependent response in the number of animals producing sperm. At the most effective hCG dose (300 IU, 100% of the male toads produced sperm, compared to only 35% for the best LHRH dose tested (4.0 micrograms. In addition to having a greater number of responders (P  Conclusion There is a clear dichotomy between the two hormones’ physiological responses on gamete production and stimulation of amplexus. Understanding how these two hormones influence physiology and reproductive behaviors in amphibians will have direct bearing on establishing similar breeding protocols for endangered species.

  19. Dose- and time-related changes in aerobic metabolism, chorionic disruption, and oxidative stress in embryonic medaka (Oryzias latipes): Underlying mechanisms for silver nanoparticle developmental toxicity

    Energy Technology Data Exchange (ETDEWEB)

    Wu Yuan, E-mail: uyuan@mail.ustc.edu.cn [Department of Public Health, Anhui Medical University, Hefei (China); State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing (China); Zhou Qunfang [State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing (China)

    2012-11-15

    Silver nanoparticles (AgNPs) are widely employed in commercial products, and are thus inevitably released into the aquatic environment. Many studies have indicated that AgNPs could induce toxicological effects on embryonic fish. To understand the mechanism of AgNP developmental toxicity, we determined the effects of AgNPs on the egg membrane, aerobic metabolism, antioxidant system, lipid peroxidation, as well as reactive oxygen species (ROS) and singlet oxygen ({sup 1}O{sub 2}) generation in early-life medaka fish (Oryzias latipes). AgNP treatment at 62.5-1000 {mu}g/L caused significant increase in retarded development and abnormalities. Destruction of the surface ornamentation and egg envelope was observed at a higher AgNP concentration ({>=}125 {mu}g/L) using light microscopy and scanning electron microscopy. A dose-dependent increase in lactate dehydrogenase activity, an indicator of anaerobic metabolism, and superoxide dismutase activity was observed in the treated embryos. In contrast, the total reduced glutathione level decreased. A high thiobarbituric acid reactive substance concentration was generated upon AgNP exposure from day 1 to day 7 postfertilisation. The biochemical parameters suggested that oxidative stress was induced by the AgNPs. Unexpectedly, a dose-dependent reduction in ROS and {sup 1}O{sub 2} generation upon high AgNP exposure ({>=}250 {mu}g/L) was observed. Although the morphological damages induced by the AgNPs were irreversible, restorable antioxidant defenses were noted in the well-developed embryos. This finding supported the idea that the stage of morphogenesis and organogenesis is a critical window to chemical exposure or environmental stress. Overall, the results suggested that hypoxia, disturbed egg chorion, and oxidative stress are mechanistically associated with AgNP toxicity in embryonic fish.

  20. The innermost chorionic layer of Drosophila. I. The role of chorin octamers in the formation of a family of interdigitating crystalline plates.

    Science.gov (United States)

    Akey, C W; Edelstein, S J

    1987-02-20

    The innermost chorionic layer (ICL) within egg shells of Drosophila melanogaster is composed of thin, abutting three-dimensional crystalline plates which form a closed, membrane-like sheath. Collectively, the crystals within the sheath appear to form a family of related three-dimensional crystals in space group C222; however, specimens prepared for electron microscopy are actually two-dimensional crystals in c222. The projected structures of the negatively stained crystals have been studied by minimal dose electron microscopy employing image reconstruction methods. Thin sections indicate that unit cells within the ICL are composed of paired layers; top and bottom layers are related by centrally located 2-fold axes, aligned parallel to the surface of the ICL. The most probable structural unit of the crystals is a tetramer of chorin dimers with a point group symmetry of 222, which is denoted a chorin octamer. Projection maps were computed from average transforms of two-dimensional crystals for delta (the primitive unit cell angle) equal to 84 degrees, 90 degrees and 97 degrees (+/- 1.5 degrees). The maps indicate that the molecular transitions responsible for the observed family of crystals involve concerted intramolecular rearrangements about molecular 2-fold axes. The significance in vivo of the family of crystals within the ICL is not known; however, structural considerations suggest that the observed polymorphism may reflect one facet of an intrinsic bonding flexibility of the ICL octamer that may play a role in the formation of interplate junctions and the assembly of a continuous closed sheath. The ICL may therefore serve as a structural bridge between the vitelline membrane-wax layer and the endochondrial floor, allowing the larva to shed the inner egg shell layers during hatching.

  1. Evaluation of dual trigger with gonadotropin-releasing hormone agonist and human chorionic gonadotropin in improving oocyte maturity rates: A prospective randomized study

    Directory of Open Access Journals (Sweden)

    Nalini Mahajan

    2016-01-01

    Full Text Available BACKGROUND: Mature oocytes are prerequisite for achieving the process of in vitro fertilization. Human chorionic gonadotropin (hCG is the standard trigger used for stimulating ovulation but is associated with ovarian hyperstimulation syndrome (OHSS. Gonadotropin-releasing hormone agonist trigger achieves oocyte maturation and lowers the incidence of OHSS, but it has limitations of higher pregnancy loss rate and miscarriage rates. Coadministration of both hormones is found to improve the pregnancy rates and the number of mature oocytes retrieved. We aimed to assess if the dual trigger is better than the conventional hCG in triggering oocyte maturation. METHODOLOGY: The study included 76 female patients aged 24–43 years who were randomly divided into two groups with 38 patients in each arm. The study included patients with antimullerian hormone (AMH 4 ng/ml and AFC/ovary >12 to avoid OHSS risk with hCG trigger. RESULTS: The study showed statistically insignificant differences between dual group versus hCG group in terms of the number of oocytes retrieved (10.0 ± 5.6 vs. 8.7 ± 5.0; P = 0.2816, the number of mature oocytes recovered (8.4 ± 5.0 vs. 7.2 ± 4.0; P = 0.2588, fertilization rate (5.9 ± 4.2 vs. 5.6 ± 3.3; P = 0.7390, and the number of usable embryos on day 3 (4.0 ± 3.0 vs. 4.0 ± 2.4; P = 0.8991. CONCLUSION: The dual trigger is equivalent to hCG in triggering oocyte maturation.

  2. Evaluation of dual trigger with gonadotropin-releasing hormone agonist and human chorionic gonadotropin in improving oocyte maturity rates: A prospective randomized study

    Science.gov (United States)

    Mahajan, Nalini; Sharma, Shilpa; Arora, Puneet Rana; Gupta, Shalu; Rani, Kumkum; Naidu, Padmaja

    2016-01-01

    BACKGROUND: Mature oocytes are prerequisite for achieving the process of in vitro fertilization. Human chorionic gonadotropin (hCG) is the standard trigger used for stimulating ovulation but is associated with ovarian hyperstimulation syndrome (OHSS). Gonadotropin-releasing hormone agonist trigger achieves oocyte maturation and lowers the incidence of OHSS, but it has limitations of higher pregnancy loss rate and miscarriage rates. Coadministration of both hormones is found to improve the pregnancy rates and the number of mature oocytes retrieved. We aimed to assess if the dual trigger is better than the conventional hCG in triggering oocyte maturation. METHODOLOGY: The study included 76 female patients aged 24–43 years who were randomly divided into two groups with 38 patients in each arm. The study included patients with antimullerian hormone (AMH) 4 ng/ml and AFC/ovary >12 to avoid OHSS risk with hCG trigger. RESULTS: The study showed statistically insignificant differences between dual group versus hCG group in terms of the number of oocytes retrieved (10.0 ± 5.6 vs. 8.7 ± 5.0; P = 0.2816), the number of mature oocytes recovered (8.4 ± 5.0 vs. 7.2 ± 4.0; P = 0.2588), fertilization rate (5.9 ± 4.2 vs. 5.6 ± 3.3; P = 0.7390), and the number of usable embryos on day 3 (4.0 ± 3.0 vs. 4.0 ± 2.4; P = 0.8991). CONCLUSION: The dual trigger is equivalent to hCG in triggering oocyte maturation. PMID:27382235

  3. Salmo salar: morfología ultraestructural de la pared del corion en ovas normales y con problemas de eclosión Salmo salar: ultrastructural morphology of chorion, from normal and with hatching problems ova

    Directory of Open Access Journals (Sweden)

    R Jaramillo

    2009-01-01

    Full Text Available El “corion duro” es un fenómeno de reciente ocurrencia y se caracteriza porque algunos alevines con ojo no son capaces de degradar el corion que les rodea o lo hacen sólo de manera parcial, produciéndose en ambos casos la muerte del alevín. Este fenómeno ha causado fracasos reproductivos importantes en algunas pisciculturas del sur de Chile, desconociéndose hasta ahora la razón de su ocurrencia. En este estudio se realizaron mediciones del grosor del corion de Salmo salar así como de las fibras que forman parte de éste en ovas fertilizadas, no fertilizadas, con desarrollo normal y ovas que no lograron eclosionar. Los resultados obtenidos indican que las leves variaciones observadas en el grosor del corion entre los diferentes estados de desarrollo de las ovas estudiadas no explican la ocurrencia del “corion duro”. Sin embargo, la disposición de las fibras proteicas que forman el estrato interno del corion parece ser la respuesta a este fenómeno. Las ovas que no eclosionan presentan “corion duro” y exhiben un estrato interno carente de poros a diferencia de lo que se observa en las ovas que desarrollan normalmente y que logran la eclosión. Se propone que problemas en la síntesis de alguna de las sub-unidades que forman la coriogenina serían los responsables de la formación de un estrato interno carente de poros, siendo ésta la razón del “corion duro”.Either eyed alevins unable to digest the chorion or those digesting it partially, are both suffering the “hard chorion” phenomenon; in both cases alevins can not survive producing high mortality rates. During recent years, the salmon farming industry in Southern Chile has been facing massive mortality events caused by “hard chorion”. Since “hard chorion” is a recently observed phenomenon there are no literature reporting its origin. The aim of this study was to find differences in the ultrastructure of chorion fibers from Salmo salar ova at different

  4. 应用SNP微阵列检测稽留流产组织绒毛染色体异常%SNP microarray analysis of retention abortion chorionic villus

    Institute of Scientific and Technical Information of China (English)

    王玲红; 任晨春; 田英; 王文靖; 王承缙; 陈淑琴; 梁玥宏; 张海霞; 张月香

    2015-01-01

    Objective To compare villus cell culture and karyotype analysis with single nucleotide polymorphism (SNP) microarray technology for the detection of chorionic villus chromosome in patients with retention of abortion.Methods Forty cases were analyzed with the two methods.Results Chorionic villus culturing was successful in 29 cases,among which 10 were found to have an abnormal karyotypes.For the SNP microarray analysis,all 40 cases were successful,among which 16 were shown to have an abnormal molecular karyotype.Conclusion SNP microarray technology is highly accurate and specific,which is particularly suitable for the detection of chromosomal deletions or duplications,uniparental disomy,lowpercentage mosaicism and other chromosomal abnormalities.It has provided an effective supplement to the conventional chorionic villus culture and karyotype analysis.%目的 比较用绒毛细胞培养核型分析与单核苷酸多态性(single nucleotide polymorphism,SNP)微阵列技术对稽留流产绒毛进行染色体检测的优缺点.方法 对确诊为稽留流产的40位孕妇应用上述两种方法检测绒毛染色体.结果 绒毛培养成功29份,发现核型异常10例;SNP微阵列均检测成功,发现异常分子核型16例.结论 SNP微阵列检测准确率高,特异性强,尤其能检测微缺失或重复、单亲二倍体、低比例嵌合体等染色体异常,是对传统的绒毛细胞培养核型分析的有效补充.

  5. Plasma progesterone profile and conception rate following exogenous supplementation of gonadotropin-releasing hormone, human chorionic gonadotropin, and progesterone releasing intra-vaginal device in repeat-breeder crossbred cows

    OpenAIRE

    N. K. J. Pandey; Gupta, H. P.; Shiv Prasad; S. K. Sheetal

    2016-01-01

    Aim: This study was designed to evaluate the effect of gonadotropin-releasing hormone (GnRH), human chorionic gonadotropin (hCG), and progesterone impregnated intra-vaginal device on progesterone profile and conception rate in repeat-breeding crossbred cows. Materials and Methods: Repeat-breeding crossbred cows aged 3-8 years (n=32), lactating and negative to white side test were randomly divided into four groups: Group 1 (Control, n=8), Group 2 (GnRH at 10 μg i.m, n=8), Group 3 (hCG at 15...

  6. 125I-labelled human chorionic gonadotrophin (hCG) as an elimination marker in the evaluation of hCG decline during chemotherapy in patients with testicular cancer

    OpenAIRE

    Christensen, T. B.; Engbaek, F; Marqversen, J; Nielsen, S I; Kamby, C.; Maase, H von der

    1999-01-01

    The rate of reduction in the concentration of serum human chorionic gonadotrophin (hCG) following chemotherapy for germ cell tumours may follow a complex pattern, with longer apparent half-life during later stages of chemotherapy, even in patients treated successfully. The commonly used half-life of less than 3 days for hCG to monitor the effect of chemotherapy in patients with germ cell tumours of the testis may represent too simple a model. 125I-labelled hCG was injected intravenously in 27...

  7. Synchronization of ovulation in crossbred dairy heifers using gonadotrophin-releasing hormone agonist, prostaglandin F2a and human chorionic gonadotrophin or estradiol benzoate

    Directory of Open Access Journals (Sweden)

    Castilho C.

    2000-01-01

    Full Text Available Girolando (Gir x Holstein is a very common dairy breed in Brazil because it combines the rusticity of Gir (Bos indicus with the high milk yield of Holstein (Bos taurus. The ovarian follicular dynamics and hormonal treatments for synchronization of ovulation and timed artificial insemination were studied in Girolando heifers. The injection of a gonadotrophin-releasing hormone (GnRH agonist was followed 6 or 7 days (d later by prostaglandin F2a (PGF2a. Twenty-four hours after PGF2a injection either human chorionic gonadotropin (hCG, GPh-d6 and GPh-d7 groups or estradiol benzoate (EB, GPE-d6 and GPE-d7 groups was administered to synchronize ovulation and consequently allow timed artificial insemination (AI 24 and 30 h after hCG and EB injection, respectively. Follicular dynamics in Girolando heifers was characterized by the predominance of three follicular waves (71.4% with sizes of dominant follicles (10-13 mm and corpus luteum (approximately 20 mm similar to those for Bos indicus cattle. In the GnRH-PGF-hCG protocol, hCG administration induced earlier ovulation (67.4 h, P<0.01 compared to the control group (GnRH-PGF and a better synchronization of ovulation, since most of it occurred within a period of 12 to 17 h. Pregnancy rate after timed AI was 42.8 (3/7, GPh-d6 to 50% (7/14, GPh-d7. In contrast, estradiol benzoate (GnRH-PGF-EB protocol synchronized ovulation of only 5 of 11 heifers from the GPE-d7 group and of none (0/7 from the GPE-d6 group, which led to low pregnancy rates after timed AI (27.3 and 0%, respectively. However, since a small number of Girolando heifers was used to determine pregnancy rates in the present study, pregnancy rates should be confirmed with a larger number of animals.

  8. Chromosome karyotype analysis of chorionic villi in 84 patients with early spontaneous abortion%84例自然流产绒毛细胞培养及染色体核型分析

    Institute of Scientific and Technical Information of China (English)

    沈素岩; 张金艳

    2016-01-01

    目的:探讨自然流产患者绒毛细胞培养及染色体核型分析的重要性及临床意义,为下次妊娠做出指导。方法:对84例患者在无菌条件下取绒毛组织,进行细胞培养及染色体核型分析。结果:实施染色体核型分析的患者84例,女胎明显多于男胎,有统计学意义;异常核型43例,正常核型41例,二者男女性别比例无统计学意义;异常核型以染色体数目异常为主(83.72%),以三体最常见(58.33%)。结论:绒毛细胞培养染色体核型分析具有临床意义,对下次妊娠指导起了重要作用,为优生工作的开展提供了重要指标。%Objective:To investigate the importance and clinical significance of chromosome karyotype analysis from chorionic villi in patients with spontaneous abortion, and to provide guidance for the next pregnancy.Methods:chorionic villi cultivation and chromosome karyotype analysis were done from 84 cases of spontaneous abortion.Results:Among 84 cases of fetus, the female fetal were significantly more than male fetal, with statistical significance.43 cases of abnormal karyotype and 41 cases with normal karyotype were checked out. The ratio of sex had no statistical significance;Numerical abnormality was the majar part of chromosome abnormality (83.72%),and the most common cases were autosomal trisomies (58.33%).Conclusion: Chromosome karyotype analysis from chorionic villi has clinical significance.And it had important function for the next pregnancy guidance.It could provide the important index for the development of the eugenic work.

  9. Smoking cessation early in pregnancy and birth weight, length, head circumference, and endothelial nitric oxide synthase activity in umbilical and chorionic vessels: an observational study of healthy singleton pregnancies

    DEFF Research Database (Denmark)

    Andersen, Malene R; Simonsen, Ulf; Uldbjerg, Niels;

    2009-01-01

    BACKGROUND: Reduced production of the vasodilator nitric oxide (NO) in fetal vessels in pregnant smokers may lower the blood flow to the fetus and result in lower birth weight, length, and head circumference. The present study measured endothelial NO synthase (eNOS) activity in fetal umbilical...... and chorionic vessels from nonsmokers, smokers, and ex-smokers and related the findings to the fetal outcome. METHODS AND RESULTS: Of 266 healthy, singleton pregnancies, 182 women were nonsmokers, 43 were smokers, and 41 stopped smoking early in pregnancy. eNOS activity and concentration were quantified...... in endothelial cells of the fetal vessels. Cotinine, lipid profiles, estradiol, l-arginine, and dimethylarginines that may affect NO production were determined in maternal and fetal blood. Serum cotinine verified self-reported smoking. Newborns of smokers had a lower weight (P

  10. Epitope mapping from real time kinetic studies – Role of cross-linked disulphides and incidental interacting regions in affinity measurements: Study with human chorionic gonadotropin and monoclonal antibodies

    Indian Academy of Sciences (India)

    Nonavinakere Seetharam Srilatha; P Tamil Selvi; Gundlupet Satyanarayana Murthy

    2005-06-01

    Real time kinetic studies were used to map conformational epitopes in human chorionic gonadotropin (hCG) for two monoclonal antibodies (MAbs). The epitopes were identified in the regions (5–14 and 55–62). The association rate constant (+1) was found to be altered by chemical modification of hCG, and the ionic strength of the reaction medium. Based on these changes, we propose the presence of additional interactions away from the epitope-paratope region in the hCG-MAb reaction. We have identified such incidental interacting regions (IIRs) in hCG to be the loop region 35–47 and 60–84. The IIRs contribute significantly towards the of the interaction. Therefore, in a macromolecular interaction of hCG and its MAb, is determined not only by epitopeparatope interaction but also by the interaction of the nonepitopic-nonparatopic IIRs. However, the specificity of the interaction resides exclusively with the epitope-paratope pair.

  11. Significance of chorionic villus karyotype analysis for abnormal early pregnancy ultrasound screening%早孕期超声筛查异常行绒毛核型分析的意义

    Institute of Scientific and Technical Information of China (English)

    吴坚柱; 谢英俊; 陈宝江; 陈健生; 林少宾

    2011-01-01

    目的 探讨早孕期超声筛查异常行绒毛核型分析的意义.方法 对65例早孕期超声筛查发现异常的孕妇行绒毛核型分析.结果 在65例早孕期超声筛查异常孕妇的胎儿绒毛核型中,检出异常核型28例,异常率为43.08%,主要为18-三体、21-三体和45,X.在异常的超声指征中,全身皮下水肿7例,5例核型异常;全身皮下水肿合并其他异常9例,8例核型异常;胚胎停育11例,6例核型异常;鼻骨异常合并其他异常3例,2例核型异常;NT增厚18例,4例核型异常;NT增厚合并其他异常6例,4例核型异常;多发畸形8例,3例核型异常;淋巴水囊瘤1例有核型异常.结论 早孕期超声筛查异常孕妇的胎儿染色体异常率高,对其行绒毛核型分析是必要的.%Objective: To study the significance of chorionic villus karyotype analysis for abnormal early pregnancy ultrasound screening. Methods: Chorionic villus karyotype analysis were done for 65 cases of abnormal early pregnancy ultrasound screening of pregnant women. Results: 28 cases of abnormal karyotypes were detected in 65 cases of fetal chorionic villus karyotypes from pregnant women with abnormal early pregnancy ultrasound screening. The abnormal rate was 43.08%, the mainly abnormal karyotypes were trisomy 18, trisomy 21 and 45, X. Of the indications of abnormal ultrasound systemic subcutaneous edema was found in 7 cases, 5 cases of them had abnormal karyotypes; systemic subcutaneous edema associated with other abnormalities in 9 cases, 8 cases with abnormal karyotypes; embryo damage in 11 cases, 6 cases with abnormal karyotypes; nasal bone abnormalities associated with other abnormalities in 3 cases, 2 cases with abnormal karyotypes; nuchal translucency thickening in 18 cases, 4 cases with abnormal karyotypes; nuchal translucency thickening associated with other abnormalities in 6 cases, 4 cases with abnormal karyotypes; multiple malformations in 8 cases, 3 cases with abnormal karyotypes; lymph

  12. Iodine-123-labeled radiotracers for cardiovascular and testicular imaging studies: labeling of phenyl fatty acids for myocardial studies, fibronectin for thrombus localization, and human chorionic gonadotropin for testicular studies

    International Nuclear Information System (INIS)

    Development and evaluation of iodine-123-labeled compounds for potential applications in certain imaging studies are described. A new method utilizing an organothallium intermediate has been developed to radioiodinate phenyl fatty acids. The method is simple, and consistently high yields (80 to 90%) can be obtained on a routine basis. The biodistribution patterns of the product prepared by this method were found to be very similar to those produced by the direct electrophilic substitution method. Radioiodinated fibronectin (FN) localized in experimental thrombi and pulmonary emboli. In dogs the deep-vein thrombi could be visualized within 3 hr of postinjection of the tracer. Radioiodinated human chorionic gonadotropin (HCG) localized in rat testes which permitted the scintigraphic visualization of scrotal as well as abdominal testes in rats. The localization appears to be receptor mediated

  13. Genetic diagnosis of chorionic villi from first-trimester miscarriages by SNP array%早期自然流产绒毛组织SNP-array遗传学诊断研究

    Institute of Scientific and Technical Information of China (English)

    罗春玉; 胡平; 王艳; 杨吟秋; 徐青; 骆潇洁; 李璃; 孟露露; 马定远; 许争峰

    2014-01-01

    Objective:To investigate the clinical value of single nucleotide polymor-phism array ( SNP-array) in the genetic diagnosis of chorionic villi from first-trimester miscar-riages. Methods:A cohort of 82 patients with first-trimester miscarriage undergoing dilation and curettage were enrolled in this study. Karyotyping by standard G-banding analysis was carried out on the cultured chorionic villi cell, while SNP-array analysis was performed on genomic DNA extracted from chorionic villi. Results:72 of 82 cases were successfully analyzed by G-banding karyotyping and the remaining 10 cases were failed,while SNP-array analysis succee-ded in all of the 82 cases. G-banding detected 35 normal and 37 abnormal karyotypes. SNP-ar-ray detected 30 cases of normal and 52 abnormal karyotypes. SNP-array revealed 6 abnormal chromosomes in 10 cases without G-banding analyses. Analyses from 12 cases by G-banding were discordant with those by SNP-array. SNP-array detected 4 cases of uniparental disomies ( UPD) . Conclusion:SNP-array could be used for genetic diagnosis of the first-trimester mis-carriages,due to its high-accuracy,high-throughput and rapid-analysis.%目的:初步探讨单核苷酸多态性阵列( SNP-array)在早期流产绒毛遗传学诊断中的临床应用价值。方法:选取临床诊断为早期自然流产的82例患者,刮宫术后获取绒毛组织,行常规绒毛细胞培养G显带核型分析,并同时提取绒毛组织DNA进行SNP-ar-ray检测,比较两者的检测结果。结果:常规绒毛细胞培养G显带核型诊断成功率87.8%(72/82),SNP-array诊断成功率为100%(82/82)。 G显带分析获得结果72例,核型正常35例,核型异常37例,异常率51.4%(37/72)。82例SNP-array分析结果中,核型正常30例,核型异常52例,异常率63.4%(52/82)。 G显带分析失败的10例标本中, SNP-array检出6例异常;G显带与SNP-array结果不符的12例中,包括2例全基因组单亲二倍体( uniparental disomy,UPD),2例是部分染色

  14. Ex vivo expansion of hematopoietic stem- and progenitor cells from cord blood in coculture with mesenchymal stroma cells from amnion, chorion, Wharton's jelly, amniotic fluid, cord blood, and bone marrow.

    Science.gov (United States)

    Klein, Caroline; Strobel, Julian; Zingsem, Jürgen; Richter, Richard H; Goecke, Tamme W; Beckmann, Matthias W; Eckstein, Reinhold; Weisbach, Volker

    2013-12-01

    In most cases, the amount of hematopoietic stem and progenitor cells (HSPCs) in a single cord blood (CB) unit is not sufficient for allogenic transplantation of adults. Therefore, two CB units are usually required. The ex vivo expansion of HSPCs from CB in coculture with mesenchymal stroma cells (MSCs) might be an alternative. It was investigated, whether bone marrow-derived MSCs, which have to be obtained in an invasive procedure, introduce a further donor and increases the risk of transmissible infectious diseases for the patient can be replaced by MSCs from amnion, chorion, Wharton's jelly, amniotic fluid, and CB, which can be isolated from placental tissue which is readily available when CB is sampled. In a two-step ex vivo coculture mononuclear cells from cryopreserved CB were cultured with different MSC-feederlayers in a medium supplemented with cytokines (stem cell factor, thrombopoietin [TPO], and granulocyte colony-stimulating factor). Expansion rates were analyzed as well, by long-term culture-initiating cell (LTC-IC) and colony-forming unit (CFU) assays, as by measuring CD34(+)- and CD45(+)-cells. Due to the comparably low number of 5×10(2) to 1×10(4) CD34(+)-cells per cm(2) MSC-monolayer, we observed comparably high expansion rates from 80 to 391,000 for CFU, 70 to 313,000 for CD34(+)-, and 200 to 352,000 for CD45(+)-cells. Expansion of LTC-IC was partly observed. Compared to the literature, we found a better expansion rate of CD34(+)-cells with MSCs from all different sources. This is probably due to the comparably low number of 5×10(2) to 1×10 CD34(+)-cells per cm(2) MSC-monolayer we used. Comparably, high expansion rates were observed from 80 to 391,000 for CFUs, 70 to 313,000 for CD34(+)-, and 200 to 352,000 for CD45(+)-cells. However, the expansion of CD34(+)-cells was significantly more effective with MSCs from bone marrow compared to MSCs from amnion, chorion, and Wharton's jelly. The comparison of MSCs from bone marrow with MSCs from CB and

  15. Plasma progesterone profile and conception rate following exogenous supplementation of gonadotropin-releasing hormone, human chorionic gonadotropin, and progesterone releasing intra-vaginal device in repeat-breeder crossbred cows

    Science.gov (United States)

    Pandey, N. K. J.; Gupta, H. P.; Prasad, Shiv; Sheetal, S. K.

    2016-01-01

    Aim: This study was designed to evaluate the effect of gonadotropin-releasing hormone (GnRH), human chorionic gonadotropin (hCG), and progesterone impregnated intra-vaginal device on progesterone profile and conception rate in repeat-breeding crossbred cows. Materials and Methods: Repeat-breeding crossbred cows aged 3-8 years (n=32), lactating and negative to white side test were randomly divided into four groups: Group 1 (Control, n=8), Group 2 (GnRH at 10 µg i.m, n=8), Group 3 (hCG at 1500 IU i.m., n=8), and Group 4 (progesterone impregnated intra-vaginal device at 958 mg, n=8). All the treatme nts were given on 5th daypostbreeding and in Group 4 intra-vaginally implanted device was withdrawn on 9th day (i.e., implant inserted for total 4 days) of the estrous cycle. Blood samples were collected on day 0, 5, 10, 15, and day 20 of estrous cycle, and plasma was separated for progesterone estimation. Results: Accessory corpus luteum was not formed in crossbred cows of Group4 and control group. However, total 6 and 8 accessory corpora lutea were found in Group 2 and Group 3, respectively. In pregnant cows, the plasma progesterone concentration increased continuously from day 0 to day 20. In non-pregnant cows, it increased from day 0 to day 15 and then declined. The conception rate on day 60 in Group 1, Group 2, Group 3, and Group 4 was 37.5%, 50%, 75%, and 37.5%, respectively. Conclusions: Treating repeat-breeder cows with hCG is effective in increasing conception rate by developing accessory corpora lutea and higher progesterone level.

  16. Human endometrial milk fat globule-epidermal growth factor 8 (MFGE8) is up regulated by estradiol at the transcriptional level, and its secretion via microvesicles is stimulated by human chorionic gonadotropin (hCG)

    KAUST Repository

    Sarhan, Abbaa

    2013-10-17

    Objective: We have recently showed that MFGE8, a novel epithelial cell protein in the human endometrium, upregulated during the window of implantation. We hypothesized that MFGE8 may act as a key modulator of endometrial remodeling and trophoblast invasion. The aims of this study were (i) to investigate the in vitro regulation of human endometrial epithelial cells MFGE8 transcription, translation, and secretion by sex steroids and hCG; and (ii) to examine the possibility of MFGE8 secretion via microvesicles. Design: Experimental in vitro study using Ishikawa cells. Setting: University center. Interventions: Treatment with estradiol (E2), progesterone (P4), and human chorionic gonatropin (hCG). Main outcome measures: MFGE8 mRNA and protein expression, and identification of secreted microvesicles by mass spectrometry (MS) and immunoblotting. Results: E2, but not P4 or hCG, significantly upregulated MFGE8 mRNA expression. hCG significantly increased MFGE8 secretion. Microvesicels obtained after ultracentrifugation were visualized with atomic force microscopy ranging from ~100 to 200 nm. In addition to the expected 46 kD protein, the microvesicles contained a second form of secreted MFGE8 measuring ~30 kD which was confirmed by MS. Conclusions: We demonstrated (i) dual effects of E2 and hCG on the regulation of MFGE8, and (ii) MFGE8 protein secretion in association with microvesicles. MFGE8 has the potential to modulate endometrial function and implantation via exocrine and/ or paracrine-autocrine effects. To the best of our knowledge, this is the first demonstration of microvesicular secretion of any regulatory protein by endometrial epithelial cells, providing initial evidence suggestive of microvesicular participation in cellular trafficking information in the non-pregnant and pregnant endometrium.

  17. Effects of equine chorionic gonadotrophin on follicular, luteal and conceptus development of non-lactating Bos indicus beef cows subjected to a progesterone plus estradiol-based timed artificial insemination protocol

    Directory of Open Access Journals (Sweden)

    Paulo Pitaluga Costa e Silva Filho

    2013-08-01

    Full Text Available The aim of this study was to evaluate the effects of equine chorionic gonadotropin (eCG on ovarian follicular responses, corpus luteum (CL development and conceptus length on day 16 after timed artificial insemination (TAI. A total of 124 cows at day 0 (D0 received 2 mg of estradiol benzoate (EB and the insertion of a progesterone (P4 intravaginal device. Eight days later, the device was removed, and cows received 0.15 mg of prostaglandin and 0.5 mg of estradiol cypionate (EC, and were randomly assigned to 1 of 2 treatments: eCG (n=60, in which cows received 300 U of eCG; and control (n=64. Cows were TAI 48 h after P4 device removal. The diameter of the largest follicle (LF present on D8 and D10 and of CL on D15 and D26 were measured. Conceptus recovered rate, conceptus length, CL diameter and weight were determined at slaughter on D26. Plasma P4 concentration was determined on D15 and D26. Follicular growth from D8 to D10 (P=0.03, the diameter of CL at D15 (P=0.03 and D26 (P=0.003 and the CL weight at day 26 (P=0.04 were greater in the eCG group than the control. However, there was no effect of eCG treatment on oestrus occurrence, conceptus recovery rate and length, or P4 concentrations on either D15 or D26. In conclusion, although eCG increases follicular responses and the diameter of the CL, this gonadotropin treatment does not influence the length of the conceptus or the P4 concentration on the subsequent oestrus cycle.

  18. Expression of tumor necrosis factor receptor 1 protein in chorionic villi of pregnant women with UESA%不明原因自然流产绒毛组织TNFR1的表达

    Institute of Scientific and Technical Information of China (English)

    闫春芳; 于学文; 王立琴; 马瑞霞; 李旭

    2009-01-01

    目的 探讨肿瘤坏死因子受体1在绒毛组织中的表达及其与不明原因早期自然流产的关系.方法 采用共聚焦激光显微镜和免疫组化技术,对31例妊娠6~10周不明原因早期自然流产患者(流产组)和30例同期妊娠的健康妇女(对照组)绒毛组织中肿瘤坏死因子受体1的表达进行半定量和定位检测.结果 共聚焦显微镜观察结果为:流产组绒毛间质、血管内皮、细胞滋养层、合体滋养层细胞肿瘤坏死因子受体1表达荧光强度分别为35.99±6.35、44.11±11.89、51.40±15.26和50.76±15.74,对照组分别为32.64±5.01、42.82±8.13、49.67±10.19和48.70±10.40.流产组绒毛间质肿瘤坏死因子受体1表达荧光强度高于对照组,有显著性差异(t=2.208,P0.05).免疫组化结果为:流产组绒毛间质、血管内皮、细胞滋养层及合体滋养层细胞肿瘤坏死因子受体1表达率分别为100.00%(31/31)、80.65%(25/31)、100.00%(31/31)和90.32%(28/31),对照组分别为75.00%(18/24)、58.33%(14/24)、100.00%(24/24)和95.83%(23/24),流产组绒毛间质肿瘤坏死因子受体1阳性表达率明显高于对照组,经Fisher's精确概率法检验,差异具有显著性(P=0.001).免疫组化还显示:流产组中,与合体滋养层接触的细胞滋养层和血管内皮细胞肿瘤坏死因子受体1阳性表达增强.结论 绒毛间质细胞肿瘤坏死因子受体1表达水平的升高,可能通过调节肿瘤坏死因子-α的效应,导致胎儿界面局部绒毛组织间质细胞的功能改变或损害;部分细胞滋养层及血管内皮细胞肿瘤坏死因子受体1表达水平的升高加速了细胞滋养层细胞的程序性死亡,使细胞滋养层生长受限制,可能与不明原因早期自然流产的发生有关.%Objective To investigate expression of tumor necrosis factor receptor 1 (TNFR1) in chorionic villi of pregnant women with unexplained early spontaneous abortion(UESA) and relationship of the expression with UESA

  19. Antiequine chorionic gonadotropin (eCG) antibodies generated in goats treated with eCG for the induction of ovulation modulate the luteinizing hormone and follicle-stimulating hormone bioactivities of eCG differently.

    Science.gov (United States)

    Hervé, Virginie; Roy, François; Bertin, Jean; Guillou, Florian; Maurel, Marie-Christine

    2004-01-01

    In dairy goats, treatments associating a progestogen and the equine chorionic gonadotropin (eCG) are the easiest way to induce and synchronize estrus and ovulation and to permit artificial insemination (AI) and/or out of season breeding. From the first treatment, the injection of eCG induces, in some females, the production of anti-eCG antibodies (Abs) that will interfere with the effectiveness of subsequent treatments. These anti-eCG Abs delay the preovulatory LH surge and the ovulation time, leading to poor fertility of the treated females. In this study, by in vitro bioassays, we show that anti-eCG Abs can positively or negatively modulate the LH and/or FSH bioactivities of eCG. Moreover, the modulation level of eCG bioactivity does not depend on the anti-eCG Ab affinity for eCG, as shown by surface plasmon resonance technology. The specificity of anti-eCG Abs tested by competitive ELISA highlighted the importance of a glycan environment in the recognition mechanism, especially the sialic acids specific to eCG. The different effects of anti-eCG Abs on eCG bioactivities could be explained by two hypotheses. First, steric hindrance preventing the interaction of eCG with its receptors would explain the inhibitory effect of some anti-eCG Abs; second, a conformational change in eCG by anti-eCG Abs could induce inhibition or potentiation of eCG bioactivities. It is significant that these modulations of eCG bioactivities by anti-eCG Abs impact mainly on the FSH bioactivity of eCG, which is essential for ovarian stimulation and subsequent fertility after treatment and AI, and to a lesser extent on LH bioactivity.

  20. Human Chorionic Gonadotropin Has Anti-Inflammatory Effects at the Maternal-Fetal Interface and Prevents Endotoxin-Induced Preterm Birth, but Causes Dystocia and Fetal Compromise in Mice.

    Science.gov (United States)

    Furcron, Amy-Eunice; Romero, Roberto; Mial, Tara N; Balancio, Amapola; Panaitescu, Bogdan; Hassan, Sonia S; Sahi, Aashna; Nord, Claire; Gomez-Lopez, Nardhy

    2016-06-01

    Human chorionic gonadotropin (hCG) is implicated in the maintenance of uterine quiescence by down-regulating myometrial gap junctions during pregnancy, and it was considered as a strategy to prevent preterm birth after the occurrence of preterm labor. However, the effect of hCG on innate and adaptive immune cells implicated in parturition is poorly understood. Herein, we investigated the immune effects of hCG at the maternal-fetal interface during late gestation, and whether this hormone can safely prevent endotoxin-induced preterm birth. Using immunophenotyping, we demonstrated that hCG has immune effects at the maternal-fetal interface (decidual tissues) by: 1) increasing the proportion of regulatory T cells; 2) reducing the proportion of macrophages and neutrophils; 3) inducing an M1 → M2 macrophage polarization; and 4) increasing the proportion of T helper 17 cells. Next, ELISAs were used to determine whether the local immune changes were associated with systemic concentrations of progesterone, estradiol, and/or cytokines (IFNgamma, IL1beta, IL2, IL4, IL5, IL6, IL10, IL12p70, KC/GRO, and TNFalpha). Plasma concentrations of IL1beta, but not progesterone, estradiol, or any other cytokine, were increased following hCG administration. Pretreatment with hCG prevented endotoxin-induced preterm birth by 44%, proving the effectiveness of this hormone as an anti-inflammatory agent. However, hCG administration alone caused dystocia and fetal compromise, as proven by Doppler ultrasound. These results provide insight into the mechanisms whereby hCG induces an anti-inflammatory microenvironment at the maternal-fetal interface during late gestation, and demonstrate its effectiveness in preventing preterm labor/birth. However, the deleterious effects of this hormone on mothers and fetuses warrant caution. PMID:27146032

  1. 人绒毛膜促性腺激素对实验性大鼠乳腺癌化学预防作用的分析%Chemical Prevention Role of Human Chorionic Gonadochopin on Experimental Rat Breast Cancer

    Institute of Scientific and Technical Information of China (English)

    刘艺; 王菡; 陆迪

    2012-01-01

    Objective To study human chorionic gonadochopin( hCG )on experimental rat breast cancer chemoprevention role. Methods 50 female SD rats with no birth were selected and randomly divided into early hCG intervention group,late hCG intervention group,tamoxifen( TAM )group,exposure group,and blank control group, 10 in each group. Cancer formation of the 5 groups were analyzed and compared. Results Breast tumors occurred in every group,the biggest diameter of the tumor visible was about 1. 8 cm, smallest was 0. 5 cm. Cancer rate of early hCG intervention group and exposure group was significantly different (P0.05 ). Cancer rate of late hCG intervention group and exposure group was not significantly different ( P >0.05 ). Differences in mammary gland,mammary terminal buds, catheter, vesicular buds and matured Lob leaflets of the 5 groups were statistically significant( P <0. 05 ). Conclusion hCG can effectively reduce the cancer rate of the experimental rats.%目的 探讨人绒毛膜促性腺激素(hCG)对实验性大鼠乳腺癌的化学预防作用.方法 将未经产的50只SD雌性大鼠随机分为hCG早干预组,hCG晚干预组,他莫昔芬(TAM)组,暴露组,空白对照组,各10只,对5组的成癌情况进行对比分析.结果 各组均有乳腺肿瘤发生,肉眼可见的肿瘤最大直径约1.8 cm,最小直径约0.5 cm.hCG早干预组与暴露组的成癌率比较差异有统计学意义(P<0.05),与TAM组的成癌率比较差异无统计学意义(P>0.05).hCG晚干预组与暴露组的成癌率比较差异无统计学意义(P>0.05).5组大鼠乳腺、乳腺终末芽苞、终末导管、泡状芽苞和Lob成熟的小叶相比差异有统计学意义(P<0.05).结论 hCG能够有效地降低实验性大鼠的成癌率.

  2. 21 CFR 522.1081 - Chorionic gonadotropin.

    Science.gov (United States)

    2010-04-01

    ... repeated in 14 days if the animal's behavior or examination of the ovaries per rectum indicates retreatment... Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS IMPLANTATION OR INJECTABLE DOSAGE FORM NEW ANIMAL DRUGS §...

  3. 黄体酮与绒毛膜促性腺激素对先兆流产的效果分析%The Application Effectiveness Analysis of Progesterone and Chorionic Gonadotrophin in Threatened Abortion

    Institute of Scientific and Technical Information of China (English)

    孙晓海; 李东升

    2013-01-01

    目的:通过观察黄体酮和绒毛膜促性腺激素(HCG)对先兆流产的治疗效果,探讨两种药物对妊娠结局的影响。方法:选取门诊诊断为早期先兆流产的孕妇279例,分为三组,A 组给予黄体酮20mg 肌内注射,1次/d ;B 组给予HCG 2000IU 肌内注射,1次/d ;C 组为对照组,仅予卧床休息,阴道出血停止后维持用药1周。追踪随访观察对象的妊娠成功率、足月分娩率、流产率、早产率、新生儿畸形率、新生儿并发症、新生儿体重等指标。结果:A 组与 B 组在妊娠成功率及流产率方面差异无统计学意义,但是同 C 组比较差异均有统计学意义。 A 组、B 组新生儿体重高于 C 组,差异有统计学意义。三组新生儿畸形率、新生儿并发症比较,差异均无统计学意义。结论:黄体酮与 HCG 均能改善先兆流产患者的预后,且对母儿无不良影响。%Objective :To observe the efficacy of Progesterone and Chorionic Gonadotrophin in the treatment of threat-ened abortion ,and approach the effective of two kinds of medicine in the treatment of pregnancy outcome .Methods :The patients was diagnosed as threatened abortion in early pregnant women 279 cases ,divided into 3 groups ,intramuscular injection of Progesterone 20mg ,1 times/day for group A ;intramuscular injection of HCG 2 000IU ,1 times/day for group B ;group C was control group ,only to be bed rest .The deadline for the treatment was vaginal bleeding 1 week af-ter the cessation of maintenance medication .Followed up the success rate of pregnancy observed objects ,full-term de-livery rate ,miscarriage rate ,premature delivery rate ,neonatal malformation rate ,neonatal complications ,neonatal weight and other indicators .Results :The successful pregnancy rate and abortion rate were not significance of difference between group A and group B ,but compared with the C group ,there were significant differences .The neonatal weight

  4. Messenger RNAs encoding the beta subunits of guinea pig (Cavia porcellus) luteinizing hormone (gpLH) and putative chorionic gonadotropin (gpCG) are transcribed from a single-copy gpLH/CGbeta gene.

    Science.gov (United States)

    Sherman, G B; Heilman, D F; Hoss, A J; Bunick, D; Lund, L A

    2001-06-01

    Neither gene locus nor gene sequence characterizations have been reported for the beta subunits of guinea pig (gp) LH and putative gp chorionic gonadotropin (CG). Descriptions of this locus would allow comparison with functionally relevant molecular genetic features of other species' homologous loci including the single-copy equid LH/CGbeta gene and the primate LHbeta-CGbeta gene cluster locus. Contiguous cDNA and genomic DNA fragments spanning the entire mature coding sequence of gpLHbeta mRNA, gpCGbeta mRNA and a homologous gpLH/CGbeta gene were amplified using PCR methodologies. With the exception of one silent mutation, the two cDNA and the genomic sequences were identical where they overlapped. Comparison of guinea pig coding sequence with LHbeta, CGbeta and LH/CGbeta sequences of other vertebrate species revealed the following order of similarity expressed as per cent coding sequence identity: rhinoceros LHbeta (83.6%)>pig LHbeta (81.8%)>donkey LH/CGbeta=bovine LHbeta (81.5%)> horse LH/CGbeta (80.6%)>dog LHbeta (79.7%)>human LHbeta (78.2%)>rat LHbeta (77.9%)>human CGbeta (75.8%)>turkey LHbeta (52.7%); values that are generally consistent with recently postulated phylogenetic relationships. Like the consensus mammalian LHbeta gene, the 5'-flanking region of the gpLH/CGbeta gene contains a single TATA sequence 37 bp upstream of the translation start codon. The first in-frame stop codon occurred at codon position +122 which is consistent with the 121 amino acid residue length of the consensus mammalian mature LHbeta peptide. To estimate gene copy number, full-length gpLHbeta cDNA was radiolabeled and hybridized to Southern blots of guinea pig genomic DNA digested with a panel of six restriction endonucleases. The resulting simple hybridization pattern strongly suggested that there is a single-copy gpLH/CGbeta gene. Northern analysis of total pituitary RNA using the same probe indicated that gpLHbeta transcript size is indistinguishable from that of consensus

  5. Evaluation of a second trimester triple marker screening test for fetal status using alpha-fetoprotein (aFP), human chorionic gonadotropin (hCG) and unconjugated estriol (uE{sub 3})

    Energy Technology Data Exchange (ETDEWEB)

    Mi, Seong Young; Kim, Jong Ho; Choi, Seung Hun [Chung Ang Gil Hospital, Inchon (Korea, Republic of)

    1997-07-01

    Our purpose was to assess the utility of maternal serum triple-marker screening test using alpha-fetoprotein (aFP), human Chorionic Gonadotropin (hCG) and unconjugated Estriol (uE{sub 3}) for fetal chromosomal abnormalities. 1,767 venous blood samples (4ml) between 15 and 20 week's gestation for maternal serum screening from January to October 1996, were tested with Kodak Amerix-M triple marker radioimmunoassay kits. Risk analysis was achieved with interpretive software such as Alpha (LMS, Kodak Clinical Diagnostics). Marker levels are transformed into multiples of median (MOM), which represent an interpretation of (weight regressed) patient marker levels relative to regressed median levels for stated gestation. By multivariate anaysis, the three MOM values are combined to generate a liklihood ratio. Calculation of a patient, risk is the product of liklihood ratio and age-related risk. Risk assessment is weight for maternal age. The median values of aFP, hCG and uE{sub 3} were well correlated with gestational age, respectively (r=0.94, p=0.003; r=-0.97, p=0.029; r=0.99, p<0.001, respectively). The median value of hCG were correlated withmateral age (r=0.13, p=0.04) but those of aFP and eU{sub 3} weren't (r=-0.17, p=0.22; r=0.36, p=0.09, respectively). The values of aFP, CG and uE{sub 3} between pregnancy younger than 35 years-old (n=87) and older than that (n=1640) were 51.67{+-}27.44, vs 54.65{+-}126.36, 46.45{+-}30.08 vs 51.33{+-}38.50 and 8.01{+-}11.01 vs 6.68{+-}7.23, respectively but all of them failed to show significant differences. A second-trimester risk for trisomy 21 > or = 1:270 was considered screen positive. Patients were screen positive for trisomy 21 if aFP < or 0.7 multiples of median (MOM), hCG> or 2.1 MOM and E{sub 3} < or 0.7 MOM. Patients were screen positive for neural tube defect if aFP >2.5 MOM. The initial screen-positive rate for both Down' syndrome and neural tube defect were 1.46% (26/1767); 0.73% (13/1767) with each other

  6. Apoptosis and Expression of Related Genes in Early Pregnant Chorionic Villi and Decidua%米非司酮药物流产后绒毛蜕膜细胞凋亡的相关研究

    Institute of Scientific and Technical Information of China (English)

    施晓华; 钱小泉; 张品南; 陈飞琴; 冯国飞

    2014-01-01

    Objective:To investigate the mechanism of mifepristone on apoptosis and proliferation in early pregnant chorionic villi and decidua.Methods:The specimen of early pergnant chorinic villi and decidua was obtained from 20 cases of mifepriston contragestation (study group),20 eases of requesting temination of pregnancy by curettage (the control).The paraffin sections were used to defermine apoptotic cells by TdT-mediated dUTP-biotin nick and labeling method,to identify bcl-2,bax,fas,fasL and proliferating cell nuclear antigen (PCNA) by immunohistochemistry,to demonstrate fas and fasL mRNA by in situ hybridization.Results:In the control,apoptosis cells were mainly observed in syncytiotrophoblast,but not in cytotrophoblast cell,occationally observed in decidua cells.The antigen of bax,fas,fasL were present in syncytiotrophoblast cells and decidua with lower amount.While bcl-2 antigen staning was strong in cytotrophoblastic cells and in decidual cells only.In study group,apoptotic cells were increased in syncytiotrophoblast cells of villi and visualized in decidua cells.The expression of fas,fasL and bax was also higher than that of the control.Conclusion:Mifepristone increased apoptosis in syncytiotrophoblast and decidua cells,but had no effect on the expression of bcl-2 and PCNA.%目的:探讨米非司酮对早孕绒毛蜕膜细胞凋亡、增殖的作用机制.方法:以20例药物流产患者为研究组,以20例非意愿妊娠要求行人工流产负压吸引刮宫术的患者为对照组,分别收集绒毛和蜕膜标本,应用原位末端标记法(TUNEL)检测细胞凋亡,并采用免疫组织化学方法检测bcl-2、bax、fas、fasL、增殖细胞核抗原(PCNA)5种蛋白在绒毛和蜕膜中的分布与表达强度,同时应用原位杂交法测定fas与asLmRNA的分布与表达强度.结果:凋亡细胞在正常早孕绒毛合体滋养细胞中少量存在,蜕膜中偶见;绒毛、蜕膜中bcl-2、bax、fas、fasL、PCNA均有表达.采用米非司酮药物

  7. FISH快速检测自然流产绒毛染色体非整倍体异常的临床价值%Rapid Detection of Chromosomal Aneuploidy in Chorionic Villus of Spontaneous Abortion by Fluorescence in Situ Hybridization

    Institute of Scientific and Technical Information of China (English)

    吴庆华; 史惠蓉; 解艳华; 江淼; 赵振华; 鲁宁; 孔祥东

    2012-01-01

    目的:探讨应用荧光原位杂交技术( FISH)对早期自然流产绒毛染色体非整倍体检测的临床价值.方法:对30例因自然流产行清宫术的绒毛组织行FISH分析,使用7种探针对13、16、18、21、22号和X、Y染色体进行了检测,并对这30例流产夫妇行外周血淋巴细胞染色体常规核型分析.结果:FISH分析的30例自然流产的绒毛组织中,有17例检测出了异常信号,检出率为57%,其中8例16-三体、2例22-三体、2例13-三体和5例三倍体.30例自然流产夫妇外周血淋巴细胞染色体核型未见异常.结论:FISH技术可以快速、简便地检测出流产物绒毛组织染色体非整倍体的异常,FISH技术的应用可以为自然流产夫妇遗传咨询提供重要的信息.%Objective;To evaluate the application of fluorescence in situ hybridization (FISH) for the detection of chromosomal aneuploidy in chorionic villus of spontaneous abortion. Methods:Thirty cases of chori-onic villus were analyzed by FISH, and seven probes were used to detect chromosomes 13, 16, 18, 21, 22, X, Y respectively. Results:In the thirty cases, seventeen cases were shown abnormal signals by FISH with detection rate of 57%, including eight cases of 16-trisomy, two cases of 22-trisomy, two cases of 13-trisomy and five cases of triploidy. Conclusions:FISH analysis is a rapid and easy method in the detection of chromosomal aneuploidy in chorionic villus. The application of FISH can provide important information of genetic counseling for the patients with spontaneous abortion.

  8. IVF-ET术后流产患者绒毛和蜕膜组织凋亡及相关调控蛋白的表达%Human chorionic villi and deciduas apoptosis and related modulin expression of IVF-ET failure

    Institute of Scientific and Technical Information of China (English)

    张先霞; 王繁

    2012-01-01

    目的:检测体外受精-胚胎移植(IVF-ET)术后早期自然流产患者绒毛和蜕膜组织凋亡及相关调控蛋白血管内皮生长因子(VEGF)、Bcl-2的表达,探讨其发病和基因调控机制.方法:采用免疫组织化学染色技术检测IVF-ET术后早期自然流产者(IVF-ET组)绒毛及蜕膜组织Bcl-2、VEGF的表达水平,并以同期正常早孕者(正常早孕组)的标本为对照.结果:VEGF主要表达于绒毛滋养细胞和血管内皮细胞胞浆.IVF-ET组VEGF表达明显低于正常早孕组,差异具有统计学意义(P<0.05).Bcl-2主要在绒毛合体滋养细胞上表达.IVF-ET组滋养细胞及蜕膜细胞染色较浅,表达呈弱阳性,正常早孕组染色较深,表达强阳性,差异有统计学意义(P<0.05).结论:IVF-ET术后早期自然流产者绒毛及蜕膜组织中VEGF及Bcl-2表达显著降低,提示早期妊娠中VEGF及Bcl-2的低表达可能是导致IVF-ET术后早期自然流产的原因之一.%Objective: To investigate the human chorionic villi and deciduas apoptosis and related regulatory protein (VEGF, Bc1-2) expression in early spontaneous abortion after treatment of in vitro fertilization-embryo transfer (IVF-ET) and explore the pathomechanisra of spontaneous abortion. Methods: The expression of VEGF and Bcl-2 in the chorionic villi and deciduas was detected and analyzed in 30 cases of early spontaneous abortion patients after in vitro fertilization-embryo transfer (IVF-ET) through immunohistochemisty method, Thirty cases of normal early pregnancy as control. Results: Immunohistochemistry displayed that VEGF mainly expressed in cytoplasm of villi trophocyte and vascular endothelial cell. The VEGF level of early spontaneous abortion after IVF-ET group was significantly lower than that of normal early pregnancy group (P<0.05). Bcl-2 mainly expressed in the villi syncytiotrophoblast. Trophoblasts and decidual staining were shallow and weakly positive in early spontaneous abortion after IVF-ET group. But

  9. Serum beta human chorionic gonadotropin(β-HCG) and Progesterone predict the failing intrauterine and normal intrauterine pregnancies by ROC curve%ROC曲线对妊娠结局预测模式的评价

    Institute of Scientific and Technical Information of China (English)

    杜欣莹; 孙婧; 孙红; 李彩霞; 王文君

    2011-01-01

    目的 通过监测正常旱孕、自然流产与异位妊娠妇女血清绒毛膜促性腺激素(β-HCG)及孕酮(P)水平,建立正常早孕、自然流产与异位妊娠的妊娠结局预测模式,并利用ROC曲线评价其预测妊娠结局的能力.方法 选择正常早期单胎妊娠对照组191例,自然流产患者150例、异位妊娠患者204例.用电发光免疫法测血清β-HCG及P水平,建立单独β-HCG、P以及β-HCG和P联合积分预测模式.结果 正常早孕组孕龄血清β-HCG平均值为(7812.52±3599.29)IU/L,P平均值为(35.27±12.91)nmol/L;自然流产组血清β-HCG平均值为(1298.07±1808.83)IU/L,P平均值为(6.66±4.42)nmol/L;异位妊娠组β-HCG平均值为(2118.23±2905.75)IU/L,P平均值为(6.37±5.58)nmol/L;自然流产组血清β-HCG及P水平明显低于正常早孕对照组,独立样本t检验t=21.75,P=0.000(95%CI:5925.31-7103.79)及t=28.49,P=0.000(95%CI:26.52-30.46),差异有显著性意义;异位妊娠组血清β-HCG及P水平明显低于正常早孕对照组,独立样本t检验t=17.25,P=0.000(95%CI:5045.02-60.05);通过ROC曲线分析血清β-HCG、P水平及联合积分预测妊娠结局的能力,曲线下面积分别为0.879、0.973、0.984,(P值均<0.05),95%CI分别为(0.845-0.913)、(0.959-0.986)、(0.973-0.994).结论 单独P较β-HCG水平具有更好的预测非正常妊娠的能力;联合积分预测妊娠结局的能力最强;单独P和联合积分与单独β-HCG预测妊娠结局的能力的差异有统计学意义,但二者的差异无统计学意义;随妊娠时间的延长,正常早孕β-HCG水平有迅速增加的趋势,异位妊娠有缓慢增加的趋势,而自然流产则有降低趋势;P水平不能有效区分自然流产与异位妊娠.%Objective: The current study was undertaken to find out the predictive value of human chorionic gonadotropin and Progesterone between normal intrauterine pregnancies, spontaneous abortion and ectopic pregnancy. Methods: The study evaluated 545 naturally conceived

  10. Interleukin-10 modifies the effects of interleukin-1beta and tumor necrosis factor-alpha on the activity and expression of prostaglandin H synthase-2 and the NAD+-dependent 15-hydroxyprostaglandin dehydrogenase in cultured term human villous trophoblast and chorion trophoblast cells.

    Science.gov (United States)

    Pomini, F; Caruso, A; Challis, J R

    1999-12-01

    The concentrations of tumor necrosis factor-alpha (TNFalpha) and interleukin-1beta (IL-1beta), two inflammatory cytokines in amniotic fluid, have been shown to rise during chorioamnionitis. This is probably related to activation of the immune system in order to intensify the inflammatory process and to protect the maternal and fetal organism from infectious agents. These cytokines activate the PG biosynthetic pathway in several tissues, but few studies have examined effects on PG-metabolizing enzymes. When PGs are produced by increased synthesis and/or decreased metabolism at the chorio-decidual interface, labor can be induced. Interleukin-10 (IL-10) is known to act as an antiinflammatory cytokine. The goals of this study were to evaluate the interaction of IL-10 with IL-1beta and TNFalpha on PG synthesis and to determine the effects of IL-10, IL-1beta, and TNFalpha on PG metabolism using purified cultures of villous trophoblast and chorion trophoblast cells prepared from placentas of patients at term. Cells were treated with IL-1beta and TNFalpha with or without IL-10 for various times up to 24 h. Levels of messenger ribonucleic acid (mRNA) encoding PGH synthase-2 (PGHS-2) and NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (PGDH) were quantified by Northern blotting, and PGE2 and 13,14-dihydro-15-keto-PGF2alpha (PGFM) output in the medium was measured by RIA. IL-1beta increased PGHS-2 mRNA and PGE2 output from villous and chorion trophoblasts and decreased PGDH mRNA in villous trophoblasts (all P < 0.05). These effects were reversed by IL-10. We found no change in PGHS-2 mRNA or PGE2 output in either trophoblast type treated with TNFalpha, but TNFalpha reduced PGDH mRNA in villous trophoblast, and this effect was reversed by IL-10 (both P < 0.05). We conclude that proinflammatory cytokines can influence PG output through effects on PG synthesis and metabolism and that these effects may be opposed by an antiinflammatory cytokine. These interactions may be

  11. The relationship between unexplained early spontaneous abortion and the expression of membrane tumor necrosis factor receptor 1 protein in chorionic villi%绒毛组织mTNFR1的表达与不明原因早期自然流产的关系

    Institute of Scientific and Technical Information of China (English)

    闫春芳; 马瑞霞; 王立琴; 于学文

    2011-01-01

    Objection: To investigated the relationship between unexplained early spontaneous abortion and the expression of membrane tumor necrosis factor receptor 1 protein in chorionic villi. Methods :Thirty-one women, who had unexplained spontaneous abortion at 6-10 weeks of gestational period ,were used as abortion group. The control group consisted of 30 normal pregnancy women who preferred artificial abortion at 6-10 weeks gestational period as well. Chorionic villous samples from the two groups were obtained at the time of abortion. Immunohistochemistry were used to determine the location and intensity of mTNFR1 in all the chorionic villous. Results: The expressive rate of mTNFR1 in villous stromal cells, vessel endothelial,cytotrophoblast and syncytiotrophoblast cells of abortion group were 100. 0 % (31/31) 、80. 7 % (25/31) 、100. 0% (31/31)and 90. 3% ( 28/31)respectively, and the control group were 75. 0 % (18/24)、 58. 3% (14/24)、 100. 0% (30/30)and 95. 8%(23/24)respectively. The expressive rate of mTNFR1 in villous stromal cells from the abortion group was higher than that in control group(100. 0% vs 75. 0%,P<0. 01). Whereas, there were no signification difference of mTNFR1 expressive rate in vessel endothelial, cytotrophoblast and syncytiotrophoblast cells between the two groups ( P> 0. 05) , immunohistochemistry results also reveal that the intensity of mTNFR1 expression in villous stromal cells from the abortion group were stronger than that in control group ((P<0. 01). Conclusion: The over expressing mTNFR1 in villous stromal cells, can possibly cause pathological changes or tissue damage , destruct immune balance of maternal-fetal interfaces in chorionic villi stormal locally, may responsible for unexplained early spontaneous abortion spontaneous abortion.%目的:探讨早孕绒毛组织中肿瘤坏死因子受体1(mTNFR1)的表达水平及其与不明原因早期自然流产的关系.方法:采用免疫组化技术,对31

  12. Gonadotrofina coriônica eqüina: purificação, caracterização e resposta ovariana em ovinos e suínos Equine chorionic gonadotrophin: purification, characterization and ovarian activity in ewes and gilts

    Directory of Open Access Journals (Sweden)

    José Antonio Guimarães Aleixo

    1995-01-01

    Full Text Available A gonadotrofina coriônica equina (eCG foi purificada e caracterizada com respeito ao grau de pureza e atividade biológica. A pureza de quatro preparações foi determinada por eletroforese e a atividade biológica pelo incremento do peso ovariano de ratas imaturas (40 - 50g e pela indução de ovulação em ovelhas e leitoas. A análise eletroforética revelou a presença de três bandas polipeptídicas. A atividade biológica media foi de 313 UI/mg de proteína. Sessenta e cinco (65 ovelhas, fora da estação reprodutiva, foram divididas ao acaso em dois grupos os quais receberam implantes vaginais de esponjas impregnadas com acetato de medroxiprogesterona por um período de l l a 14 dias. No grupo I (55 ovelhas, foram injetadas (IM 500UI do eCG purificado no momento da retirada das esponjas, enquanto que no grupo II (10 ovelhas foram injetadas 500UI de eCG comercial. Uma semana após a aplicação do eCG as ovelhas foram submetidas a um exame laparoscópico para avaliar o número de ovulações. Obteve-se uma média de 2,1 ± 0,3 e 1,8 ± 0,3 ovulações (P>0,05 para as ovelhas dos grupos I e II, respectivamente. De 120 leitoas pré-púberes, com peso médio de 87,2 kg, 90 (grupo I foram injetadas com 500UI do eCG purificado e, às 72 horas, 500UI de hCG (gonadotrofina coriônica humana, e 30 leitoas (grupo II não receberam injeção hormonal. Observou-se a presença de 25,9 ± 22,2 e 0,0 corpora lutea (PEquine chorionic gonadotrophin (eCG was purified and characterized with respect to its purity and bionological activity. The purity of four preparations was determined by electrophoresis, and the biological activity by increasing of the ovarian weight ot immature female rats (40-50g and induction ot ovulation of ewes and gilts. Electrophoretic analysis revealed three polipeptidic bands. The mean biological activity was 313UI/mg of protein. Sixty-five ewes, not in reproductive season, were divided randomly in two groups that received vaginal

  13. Optimal timing for human chorionic gonadotrophin administration from the point of view of the mature/immature oocytes ratio in controlled ovarian stimulation cycles%从超排周期所获成熟卵与不成熟卵比例的临床结局评议hCG诱发排卵最佳时机

    Institute of Scientific and Technical Information of China (English)

    李媛

    2012-01-01

    In current controlled ovarian stimulation protocol, usage of recombination follicle stimulating hormone and gonadotropin releasing hormone analogue can to avert from the dominant follicle selection mechanism which results in single follicle maturation and ovulation. Because of this progress, multiple follicles can he recruited and develop simultaneously, so that more oocytes could he retrieved. Whereas because of the absence of endogenous luteinizing hormone surge, human chorionic gonadotropin (hCG) has to be administrated to trigger final follicle maturation and induce ovulation. At the end of follicular phase, there would he a series of ovulatary follicles with different diameters. It is tricky to determine optimal timing of hCG administration due to lack of synchronization. The current criteria for hCG administration is arbitrary and not uniformed. Each IVF program would draft their own criteria and determine the timing according to the characteristics of each cycle. And in particular cases, immature oocyte rate is unbearable because of inappropriate hCG trigger timing. So far the relationship between hCG administration and IVF/ICSI outcome remains uncertain. It is seemingly that, generally the " trigger window" does exist and is not bitterly rigid. Based on our experience, oocytes with superior development capacity are aspirated from fairly large follicles with a diameter between 20 and 22 mm. At the same time, character of the patient, hormone levels and endometrium advancement should be considered. Because lack of knowledge of folliculogenesis and insufficiency of current monitoring methods, the exact timing of hCG administration could not be pinpointed. In the future, the development of perifollicular vascularization evaluation and serum/follicular anti-Mullerian hormone measurement will assist in identifying the optimal timing of hCG administration.

  14. Application of copy number variation analysis based on next-generation sequencing in the genetic analysis of missed abortion chorionic villi%基于高通量测序的染色体组拷贝数分析技术在流产胚胎组织遗传学诊断中的应用价值

    Institute of Scientific and Technical Information of China (English)

    戚红; 蔡莉蓉; 祝建疆; 杨锴; 闻小慧; 曾雯; 陈佳靓

    2016-01-01

    genetic analysis of missed abortion chorionic villi. Methods From August 2012 to May 2014, chorionic villi from 74 cases of missed abortion at 6-13 gestational weeks in Haidian Maternal and Child Health Hospital were collected and analyzed by karyotype analysis and NGS-CNVA. The results of the two methods were compared. Results (1) Karyotype analysis was carried out for the villi from the 74 missed abortion patients. Thirty cases were euploid, 26 cases were aneuploid, while 18 cases had structural abnormalities. The resolution of the karyotyping was 320 bands and the average report time was 22 days. (2) All of the 74 samples obtained NGS-CNVA results and the report time was 7-10 days. (3) The NGS-CNVA results of 56 cases were consistent with karyotype. Among them, 28 cases (28/56, 50%) had no copy number variants (CNV), and 19 cases (19/56, 34%)had CNV between 1 Mb and 10 Mb. 9 cases (9/56,16%) had CNV≥10 Mb found by NGS-CNVA, but not found by karyotyping. (4) According to the results of NGS-CNVA, karyotype were reviewed. The reviewed results found 7 cases with CNV<10 Mb and 3 cases with CNV≥10 Mb in 30 cases which got normal karyotype results at the first analysis. (5) Among the 18 cases of structural abnormalities, 6 cases were Robertsonian translocation. Sequencing technology could confirm the specific area of chromosome deletion/duplication in 8 cases, but could not locate them. Conclusions NGS-CNVA has lower failure rate, higher resolution, lower specimen requirement and shorter report time than karyotype analysis when used for the genetic analysis of missed chorionic villi . NGS-CNVA could be a useful genetic analysis method for the missed abortion villi.

  15. CD4、CCR5、CXCR4和DC-SIGN分子在 HIV-1感染者及正常人晚孕胎盘及早孕绒毛的表达%Experssion ofCD4, CCR5, CXCR4 and DC-SIGN in chorionic villi and human placentae with or without HIV-1 infection

    Institute of Scientific and Technical Information of China (English)

    董晓梅; 董杉; 彭淋; 蔡卫平; 禤庆山; 王辉; 王自能; 王声湧

    2012-01-01

    Objective To investigate the expression of HIV-1 receptors CD4, co-receptors CCR5, CXCR4 and DC-SIGN in human placenta and chorionic villi and to explore the mechanism of in-utero transmission of human immunodeficiency virus type 1( HIV-1). Methods 11 placentas from HIV-1 seropositive women, 13 placentas from normal placentas and 10 cases of early pregnancy abortion villi were collected. Immunohistochemistry method was used to detect the expression of CD4, CCR5, CXCR4 and DC-SIGN. Results There were individual differences of CD4 expression in placenta , the positive rate of the three groups was 70.00% , 61. 54% and 72. 73% , respectively. There was no statistical difference among three groups. All of CXCR4, CCR5, and DC-SIGN had positive expression in the placenta, they all located at the trophoblast cells and stromal of villi. The level of CXCR4, CCR5, and DC-SIGN expression in chorionic villi from first trimester were lower than those in placentas from the third trimester, the difference among the three groups was significant (t1=-4. 09,P1<0.001;t2 =-4. 80,P2<0. 001;t3 = -4. 57,P3 =0. 001). Conclusions With the expression of CD4, CCR5, CXCR4 and DC-SIGN, placenta possessed the molecular basis of HIV-1 infection. There are individual differences in the expression of CD4 molecules in trophoblast cells. The expressions of CCR5 , CXCR4 and DC-SIGN molecules in the placenta from the third trimester were higher than those in chorionic villi from first trimester, which might be related with the fact that most of MTCT occurred at the third trimester stage.%目的 明确CD4、CCR5、CXCR4和DC-SIGN分子在HIV-1不同感染状态晚孕胎盘和早孕绒毛的存在及表达情况,为探索HIV-1宫内传播的分子机制提供理论依据.方法 收集11例HIV-1感染孕妇胎盘、13例正常孕妇胎盘和10例早孕流产绒毛,免疫组化检测并比较3组孕妇胎盘或绒毛组织中HIV-1相关受体CD4、CCR5、CXCR4和DC -SIGN分子的存在

  16. 基于高通量测序的染色体组拷贝数分析技术在流产胚胎组织遗传学诊断中的应用价值%Application of copy number variation analysis based on next-generation sequencing in the genetic analysis of missed abortion chorionic villi

    Institute of Scientific and Technical Information of China (English)

    戚红; 蔡莉蓉; 祝建疆; 杨锴; 闻小慧; 曾雯; 陈佳靓

    2016-01-01

    Objective To investigate the value of copy number variation analysis based on next-generation sequencing (NGS-CNVA) in the genetic analysis of missed abortion chorionic villi. Methods From August 2012 to May 2014, chorionic villi from 74 cases of missed abortion at 6-13 gestational weeks in Haidian Maternal and Child Health Hospital were collected and analyzed by karyotype analysis and NGS-CNVA. The results of the two methods were compared. Results (1) Karyotype analysis was carried out for the villi from the 74 missed abortion patients. Thirty cases were euploid, 26 cases were aneuploid, while 18 cases had structural abnormalities. The resolution of the karyotyping was 320 bands and the average report time was 22 days. (2) All of the 74 samples obtained NGS-CNVA results and the report time was 7-10 days. (3) The NGS-CNVA results of 56 cases were consistent with karyotype. Among them, 28 cases (28/56, 50%) had no copy number variants (CNV), and 19 cases (19/56, 34%)had CNV between 1 Mb and 10 Mb. 9 cases (9/56,16%) had CNV≥10 Mb found by NGS-CNVA, but not found by karyotyping. (4) According to the results of NGS-CNVA, karyotype were reviewed. The reviewed results found 7 cases with CNV<10 Mb and 3 cases with CNV≥10 Mb in 30 cases which got normal karyotype results at the first analysis. (5) Among the 18 cases of structural abnormalities, 6 cases were Robertsonian translocation. Sequencing technology could confirm the specific area of chromosome deletion/duplication in 8 cases, but could not locate them. Conclusions NGS-CNVA has lower failure rate, higher resolution, lower specimen requirement and shorter report time than karyotype analysis when used for the genetic analysis of missed chorionic villi . NGS-CNVA could be a useful genetic analysis method for the missed abortion villi.%目的:探讨基于高通量测序的染色体组拷贝数分析(NGS-CNVA)技术在流产胚胎组织遗传学诊断中的价值。方法选择2012年8月至2014年5月间在

  17. 30例经腹绒毛活检在孕早期产前诊断中的应用分析%Analysis of application of trans-abdominal chorionic villusin sampling in the prenatal diagnosis in the first trimester

    Institute of Scientific and Technical Information of China (English)

    田丽蕴; 范琦慧

    2015-01-01

    Objective To analyze the application of trans-abdominal chorionic villus sampling in the prenatal diagnosis in the first trimester. Methods A total of 70 patients with single birth and indication of invasive prenatal diagnosis in our hospital from November 2013 to January 2015 were selected. 30 pregnant women in the first trimester was given trans-abdominal chorionic villus sampling (TA-CVS), and 40 pregnant women in the second trimester was given am-niocentesis. Surgery successful rate and pregnancy loss rate were calculated in the two groups. Results In the 30 preg-nant women undergone TA-CVS, chromosome abnormality was detected in 15 cases and induced labor was carried out for all (including 1 case of stillbirth by re-examination 1 week after the surgery of trisomy-21), including 5 cases of trisomy-21, 3 cases of trisomy-13, 5 cases of 45 XO and 2 cases of trisomy-18. The rest of pregnant women with nor-mal testing results of chromosome were traced and observed until delivery. Complications such as placental hematoma, vaginal bleeding and discharge and infants' acromesomelic dysplasia were not seen in the 30 pregnant women. Com-pared with the 40 cases receiving amniocentesis, the surgery successful rate was 100%, and the pregnancy loss rate was 3.33%, and the difference was not statistically significant (P>0.05). Conclusion Amniocentesis should be performed in 18 weeks. Therefore, TA-CVS is able to detect the problem in early stage, so as to alleviate pregnant women's emo-tional and mental pressure. TA-CVS in the first trimester is an early, safe, accurate and reliable invasive technology for prenatal diagnosis. Successful surgery in real practice depends on various aspects.%目的 分析经腹绒毛活检在早孕期产前诊断中的应用. 方法 选择我院 2013年11月~2015年1月有介入性产前诊断指征的单胎病例70例,30例孕早期孕妇行经腹绒毛活检(trans-abdominal chorionic villus sampling, TA-CVS),40例孕中

  18. Karyotype Analysis on Chorionic Villus of Early Pregnancy Loss in Relation to Different Ultrasound Findings%早期妊娠自然流产患者的超声表现与绒毛染色体核型分析

    Institute of Scientific and Technical Information of China (English)

    刘玉昆; 刘颖琳; 杜涛; 陈立斌; 刘梅兰; 陈慧; 谭剑平; 张建平

    2013-01-01

    [目的]探讨早期妊娠自然流产患者的不同超声表现与绒毛染色体异常的关系.[方法]2008年1月至2011年12月,在我院诊治的早期自然流产患者,流产前有定期B超和人绒毛膜促性腺激素(HCG)测定,流产后成功行绒毛细胞培养和染色体核型分析者共183例.根据流产前的B超分为有胎心组和无胎心组,比较两组的染色体核型分析结果有无差异.[结果]183例绒毛染色体核型分析中,检出异常核型109例(59.6%),正常核型74例(40.4%).有胎心组102例,异常染色体发生率61.8%,无胎心组81例,异常染色体发生率56.8%,两组比较无统计学差异(P>0.05).有胎心组中最常见的异常染色体为(45,X)、三倍体和16三体,而无胎心组中最常见的异常染色体为16三体,无1例(45,X)和三倍体.有胎心组中可存活常染色体三体(21三体、18三体、13三体)、(45,X)、三倍体29例,占异常染色体的(46.0%),明显高于无胎心组,仅3例,占异常染色体的6.5%,(P<0.05).在有胎心组中,可存活常染色体三体(21三体、18三体、13三体)、(45,X)、三倍体的胚芽长度平均(17.7±6.4)mm,明显大于其他异常染色体的胚芽长度(8.8±5.3)mm (P< 0.05)和正常染色体的胚芽长度(11.1±8.4) mm(P< 0.05).[结论]绒毛染色体异常是早期自然流产的主要原因,可存活常染色体三体(21三体、18三体、13三体)、(45,X)、三倍体多发生于已有胎心的自然流产者.%[ Objective ] To compare the chromosomal karyotype of chorionic villus in early pregnancy loss in relation to different ultrasound findings. [Methods] From January 2008 to December 2011, 183 patients with early pregnancy loss were evaluated by regular ultrasound and human chorionic gonadotrophin (HCG) during pregnancy and karyotyping was performed on chorionic villus after curettage. The proportion and type of chromosomal anomalies in two groups defined by presence or absence of cardiac activity before

  19. Impact of progesterone level on the day of human chorionic gonadotrophin administration on outcome of in vitro fertilization%HCG注射日孕酮水平和体外受精-胚胎移植妊娠结局的关系探讨

    Institute of Scientific and Technical Information of China (English)

    王丽娟; 胡晓东; 莫美兰; 彭月婷; 曾勇

    2013-01-01

    Objective:To evaluate the impact of progesterone level on the day of human chorionic gonadotrophin administration on pregnancy outcome of in vitro fertilization in controlled ovarian hyperstmiulation.Methods:Retrospective analysis the 889 cycles of IVF -ET which were used with down regulation long protocol in our reproductive medical centre from October 2009 to March 2011,and 56 cycles of FET which had given up fresh embryo transfer because of high progesterone levels from October 2009 to November 2011.All the patients were divided into three groups according to serum progesterone level on the day of HCG administration,group A:P < 3.17nmol/L 657 cycles; group B:3.17nmol/L≤P <4.76nmol/L 181 cycles; group C:P≥4.76nmol/L 77 cycles.Results:Compared with the other two groups,P≥ 1.5ng/ml group significantly reduced implantation and pregnancy rates but the average follical diameter and the actopic pregnancy rate was significantly higher (P < 0.01).There were no statistical differences in the number of fetilization rates,embryos cleavage rates,high quality embryos rates.Compared with C group,the patients giving up fresh embryo transfer because of high progesterone levels have higher clinical pregnancy rates of frozen thawed embryo transfer cycles.Conclusions:In controlled ovarian hyperstmiulation,the increase of progesterone level on the day of human chorionic gonadotrophin admin istration has effect only on clinical pregnancy outcome of IVF-ET,without affecting on clinical pregnancy rate of thawed embryo transfer.Therefore,the patients who have high progesterone levels maybe considered giving up fresh embryo transfer and freezing total embryo.%目的 探讨在控制性超排卵中,HCG注射日孕酮水平与体外受精-胚胎移植(IVF-ET)妊娠结局的关系.方法 回顾性分析2009年10月至2011年3月在我院生殖中心采用长方案垂体降调节行IVF-ET的889个新鲜周期,以及2009年10月至2011年11月因HCG日P≥4.76nmol/L而取

  20. 21 CFR 522.1079 - Serum gonadotropin and chorionic gonadotropin.

    Science.gov (United States)

    2010-04-01

    ... SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS IMPLANTATION OR INJECTABLE DOSAGE FORM NEW.... (i) Gilts. For induction of fertile estrus (heat) in healthy prepuberal (noncycling) gilts. (ii) Sows. For induction of estrus in healthy weaned sows experiencing delayed return to estrus. (3)...

  1. Association of human chorionic gonadotropin level in embryo culture media with early embryo development%人胚胎源性人绒毛促性腺激素在胚胎培养液中的水平与早期胚胎发育的关系

    Institute of Scientific and Technical Information of China (English)

    王海英; 张仁礼; 韩冬; 刘彩霞; 蔡佳杰; 毕燕玲; 闻安民; 全松

    2014-01-01

    Objective To investigate the association of human chorionic gonadotropin (HCG) level on day 3 of embryo culture with embryo development. Methods Spent culture media were collected from individually cultured embryos on day 3 of in vitro fertilization and embryo transfer (IVF-ET) cycles. HCG concentration in the culture media was measured using an ELISA kit and its association with embryo development was assessed. Results In the 163 samples of embryo culture media from 60 patients, HCG was positive in 153 sample (93.8%) with a mean level of 0.85 ± 0.43 mIU/ml. The concentration of hCG in the culture media increased gradually as the number of blastomeres increased (F=2.273, P=0.03), and decreased as the morphological grade of the embryo was lowered (F=3.900, P=0.02). Conclusion ELISA is capable of detecting HCG levels in spent culture media of embryos on day 3 of in vitro culture. The concentration of HCG in spent culture media is positively correlated with the status of early embryo development and implantation rate and thus serves as a useful marker for embryo selection in IVF-ET procedure.%目的:探讨人胚胎源性人绒毛促性腺激素(HCG)在第3天胚胎培养液中的表达及其与早期胚胎发育间的关系。方法收集IVF-ET患者体外第3天单个培养胚胎培养液,通过ELISA方法检测培养液中HCG浓度,比较其与胚胎数目、分级、碎片等传统评分间的关系。结果在60个病人的163份第3天胚胎培养液标本中,胎源性HCG的检测率是93.8%(153/163),表达浓度是0.85±0.43 mIU/ml。HCG浓度与胚胎卵裂球细胞数呈正相关。随着细胞数增加,培养液中HCG表达也提高(P<0.05)。随着胚胎形态评分级别的下降,培养液中HCG表达下降(P<0.05)。结论 ELISA方法能检测出第3天胚胎培养液中胎源性HCG的微量表达。胚胎培养液中胎源性HCG水平与传统胚胎形态评估有相关性,提示它有可能作为新的评估胚胎发育的无创性方法。

  2. IGF-II Y LA GONADOTROPINA CORIÓNICA REGULAN LA PROLIFERACIÓN, MIGRACIÓN E INVASIÓN DE CÉLULAS DE TROFOBLASTO HUMANO IGF-II and Chorionic Gonadotropin Regulate Proliferation, Migration and Invasion of Human Trophoblast Cells

    Directory of Open Access Journals (Sweden)

    RICARDO CABEZAS-PEREZ

    Full Text Available Son conocidas las propiedades del factor de crecimiento similar a la insulina tipo II (IGF-II y de la hormona gonadotropina coriónica (hCG en implantación y migración trofoblástica; sin embargo, los mecanismos moleculares a través de los cuales ejercen sus efectos no están completamente caracterizados. El objetivo de este estudio fue establecer la interacción potencial entre los efectos funcionales de hCG e IGF-II en la regulación de la proliferación, migración e invasión trofoblástica. Utilizando la línea celular HTR-8/SVneo de trofoblasto extravelloso se estableció que IGF-II promueve la proliferación celular y de manera novedosa se demostró que hCG, a concentraciones elevadas, es capaz de estimular la proliferación trofoblástica, a través de un mecanismo independiente al empleado por IGF-II. En contraste, la capacidad invasiva del trofoblasto fue regulada por IGF-II y hCG, planteando la existencia de un efecto aditivo en sus acciones. En conclusión, nuestros resultados demuestran el papel de hCG e IGF-II en la regulación de la proliferación e invasión del trofoblasto y plantean la existencia de interacciones a nivel de sus acciones biológicas, contribuyendo a un mejor entendimiento de la biología del trofoblasto y sus patologías.Both IGF-II and chorionic gonadotropin (hCG are important regulators of human trophoblast migration and implantation; however the molecular mechanisms are not fully understood. The purpose of this study was to determine the potential cross-talk between functional effects of hCG and IGF-II in the regulation of trophoblast proliferation, migration and invasion. Using the HTR-8/SVneo trophobast cell line we found that IGF-II stimulates cell proliferation and, for the first time we demonstrate that hCG at high doses is able to promote trophoblast proliferation through a mechanism independent of IGF-II. In contrast, trophoblast invasiveness was regulated by both IGF-II and hCG and an additive

  3. Relationship between Serum Progesterone Concetrations and Pregnancy on the day of Human Chorionic Gonadotropin Adiministration during Luteal Phase Long Protocol%黄体期长方案中人绒毛膜促性腺激素注射日血清孕酮水平与临床妊娠关系的研究

    Institute of Scientific and Technical Information of China (English)

    张胜坤; 程静; 黄朝霞; 吕杰强

    2012-01-01

    目的 探讨黄体期长方案中人绒毛膜促性腺激素(HCG)注射日血清孕酮水平与临床妊娠的关系.方法 回顾性分析2009年1月~ 2010年10月在温州医学院附属第二医院生殖中心行黄体期长方案且有完整资料的370个IVF周期.以1.0、1.5、2.0、2.5、3.0ng/ml分别作为截断值,比较不同截断值时高低孕酮组的一般临床资料、实验室指标及临床妊娠率.结果 不同截断值时,两组的临床妊娠率无明显差别(P>0.05);当以1.0、1.5、2.0和2.5ng/ml为截断值时,高孕酮组获卵数、成熟卵数、HCG日雌二醇水平高于低孕酮组,差异有统计学意义(P≤0.05).结论 1VF-ET黄体期长方案中HCG注射日孕酮水平在一定范围内升高对临床妊娠无影响,高孕酮水平可能是较多的卵泡同步发育造成的.%Objective To explore the relationship between serum progesterone concentration and pregnancy on the day of human chorionic gonadotropin ( HCC) during long protocol. Methods From January 2009 to October 2010, 370 circles with serum progesterone measurement on the day of HCG administration for final ooeyte maturation in IVF were analyzed retrospectively in Center of Reproductive Health in The Second Affiliated Hospital of Wenzhou Medical Colleage. When the cutoff of serum progesterone were set at 1.0,1.5,2.0, 2. S ,3.0ng/ml, respectively,general clinical data,laboratory results and the clinical pregnancy rate between all patients at lower and higher group at different cutoff were analyzed. Results No significant difference was found with respect to pregnant rate between lower and higher serum progesterone groups at any cutoff. However, the number of ooeyte retrieved was larger in higher group than in the lower group when the cutoff was 1.0,1.5,2.0 and 2. 5ng/ml, and so the estriol level and mature eggs. Conclusion The serum progesterone concentration on the day of HCC administration do not influence clinical pregnant rate in the long protocol. The

  4. Effect of Kaempferol on the BaP Induced Apoptosis in Human Chorionic Trophoblast Cells HTR8/SV neo%山奈酚对BaP引起的人绒毛膜外滋养层细胞HTR8/SV neo凋亡的影响

    Institute of Scientific and Technical Information of China (English)

    连立芬; 陈亚琼; 侯海燕; 曹波; 吴念

    2014-01-01

    目的:研究山奈酚对苯并芘(BaP)引起的人绒毛膜外滋养层细胞HTR8/SV neo凋亡的影响。方法:通过四甲基偶氮唑盐(MTT)法观察及测定,最终选取10μmol/L BaP和0.01,0.1,1μmol/L山奈酚共同处理人HTR8/SV neo细胞48 h,光镜下观察不同浓度BaP作用下细胞生长情况,吉姆萨(Gimesa)染色及Hochest33342荧光染色观察细胞凋亡情况,利用流式细胞术测定细胞凋亡率变化。结果:①流式细胞术测定结果表明BaP单独作用细胞存活率为(72.58±0.29)%,与0.01,0.1,1μmol/L山奈酚同时作用时细胞存活率分别为(84.96±1.34)%、(89.54±1.64)%和(91.01±1.26)%,两者同时作用组细胞存活率均高于BaP单独作用组,差异均有统计学意义(P<0.01)。②单独BaP作用可引起细胞染色质浓缩,出现凋亡小体等典型凋亡形态、细胞核致密浓染、碎裂状。结论:山奈酚对BaP引起的HTR8/SV neo细胞凋亡有明显的抑制作用。%Objective:To study the effect of kaempferol on the BaP induced apoptosis in human chorionic trophoblast cells HTR8/SV neo. Methods:10μmol/L BaP were added 0.01,0.1,1μmol/L kaempferol jointly deal with human HTR8/SV neo cells 48 h,observed cells growth under the inverted microscope,Gimesa staining and Hochest33342 fluorescent staining observed morphologic changes,determination of apoptosis rate changes by flow cytometry. Results: ①Flow cytometry results showed that BaP alone cell viability was (72.58 ±0.29)%, with 0.01,0.1,1 μmol/L kaempferol the role of cell viability was (84.96 ±1.34)%, (89.54 ±1.64)% and (91.01 ±1.26)%, respectively, compared with BaP alone group, the differences were statistically significant (P<0.01). ②BaP alone can cause cell chromatin condensation,apoptotic bodies and other typical apoptotic morphology,dense hyperchromatic nuclei,fragmentation shape. Conclusions:Kaempferol showed a protective effect on BaP induced apoptosis in

  5. 血清β人绒毛膜促性腺激素与孕早期血脂对预测妊娠高血压疾病的价值%Value of Serum β Human Chorionic Gonadotropin and Blood Lipid in Early Pregnancy in Predicting Pregnancy-induced Hypertension Disease

    Institute of Scientific and Technical Information of China (English)

    邝清梅; 张文慧; 陈文英; 李银芳

    2015-01-01

    目的:探讨血清β人绒毛膜促性腺激素(β-HCG)与孕早期血脂对预测妊娠高血压疾病的价值。方法选取2011年1月至2014年1月入住我院的妊娠高血压产妇150例,设为妊娠高血压组,另选取同期入住我院的正常健康产妇150例为正常对照组。比较两组孕妇一般性资料、孕早期(37~60天)血脂水平以及妊娠中期血清β-HCG水平,分析孕妇并发妊娠高血压的危险因素。结果妊高组患者的β-HCG、甘油三酯、胆固醇、高密度脂蛋白、低密度脂蛋白水平均明显高于正常组,差异具有统计学意义(P<0.05)。 Logistic回归分析显示,甘油三酯(OR =2.561)、高密度脂蛋白(OR =2.023)、β-HCG (OR =1.347)和低密度脂蛋白(OR =1.248)均是导致妊娠高血压的危险因素。结论血清β人绒毛膜促性腺激素与孕早期血脂均是导致妊娠高血压的危险因素,可以作为妊高症诊断的临床参考。%Objective To explore the value of serumβhuman chorionic gonadotropin (β-HCG) and blood lipid in early pregnancy in predicting pregnancy-induced hypertension disease. Methods 150 cases of pregnant women with pregnancy-induced hypertension disease admitted to our hospital from January 2011 to January 2014 were selected as gestational hypertension group, 150 cases of healthy pregnant women admitted to our hospital in same period were set as normal group. The general information, levels of blood lipid in early pregnancy (37 ~ 60 d) and serum β-HCG level in second trimester of pregnancy were compared between two groups, the risk factors of pregnancy-induced hypertension was analyzed. Results The levels ofβ-HCG, triglyceride, cholesterol, high-density lipoprotein, low density lipoprotein in gestational hypertension group were significantly higher than those in control group, with statistical difference (P<0.05). The analysis of Logistic regression showed that triglyceride (OR =2.561), high-density lipoprotein (OR =2

  6. A curva de regressão da gonadotrofina coriônica humana é útil no diagnóstico precoce da neoplasia trofoblástica gestacional pós-molar? Are curves of human chorionic gonadotropin useful in the early diagnosis of post-molar trophoblastic neoplasia?

    Directory of Open Access Journals (Sweden)

    Lúcia Regina Marques Gomes Delmanto

    2007-10-01

    Full Text Available OBJETIVO: avaliar a utilidade da curva de regressão normal da gonadotrofina coriônica humana (hCG no diagnóstico precoce de neoplasia trofoblástica gestacional pós-molar (NTG. MÉTODOS: estudo longitudinal, incluindo 105 pacientes com mola hidatiforme completa (MHC acompanhadas no Centro de Doenças Trofoblásticas de Botucatu, entre 1998 e 2005. Os títulos da hCG sérica foram mensurados quinzenalmente em todas as pacientes. Curvas individuais de regressão da hCG das 105 pacientes foram estabelecidas. A comparação entre a curva de regressão normal estabelecida em nosso serviço com as curvas individuais da hCG foi usada no rastreamento e diagnóstico (platô/ascensão de NTG. O número de semanas pós-esvaziamento quando a hCG excedeu o limite normal foi comparado com o número semanas em que a hCG apresentou platô/ascensão. RESULTADOS: das 105 pacientes com MHC, 80 apresentaram remissão espontânea (RE e 25 desenvolveram NTG. Das 80 pacientes com RE, 7 (8,7% apresentaram, inicialmente, dosagem da hCG acima do normal, mas, no devido tempo, alcançaram a remissão. Todas as 25 pacientes com NTG apresentaram desvio da curva normal da hCG em 3,8±2,5 semanas e mostraram platô ou ascensão em 8,4±2,9 semanas (pPURPOSE: to evaluate the usefulness of the normal human chorionic gonadotropin (hCG regression curve in the early diagnosis of post-molar trophoblastic neoplasia (GTN. METHODS: a longitudinal study including 105 patients with complete hydatidiform mole (CHM followed up at the Botucatu Center of Trophoblastic Diseases from 1998 to 2005. Serial serum hCG titers were measured fortnightly in all patients. Individual curves of the 105 patients were built. Comparison between the normal regression curve established at our center with individual hCG curves was used to screen and diagnose (plateau/rise GTN. The number of weeks postevacuation when hCG levels exceeded the normal limits was compared with the number of weeks when h

  7. 妊娠早期检测血清E2、P、HCG的动态变化对妊娠结局的预测价值%Dynamic Changes of Serum Estradiol,Progesterone and Human Chorionic Gonadotropin on the Predictive Value of Early Pregnancy Outcome

    Institute of Scientific and Technical Information of China (English)

    龚衍; 曾玖芝; 陈德新; 万虹

    2014-01-01

    目的:探讨妊娠早期检测孕酮(P)、雌二醇(E 2)、血绒毛膜促性腺激素(HCG)的动态变化对妊娠结局的预测价值。方法选择自然周期监测排卵后怀孕者228例,根据妊娠结局分为3组:正常妊娠组、流产组、宫外孕组。以排卵日为0d,首次检测排卵后15~17d 的血清雌二醇、孕酮、绒毛膜促性腺激素,间隔2~3d 后第2次检测,再间隔7d 第3次检测,间隔10d 以上第4次检测。比较不同间隔时间3种激素的差异。进一步比较雌二醇差值的增减比例。结果正常宫内孕组雌二醇、绒毛膜促性腺激素呈增长趋势,孕酮变化较小。流产组雌二醇、孕酮下降,绒毛膜促性腺激素有增有降,且增幅明显小于正常宫内孕组。宫外孕组雌二醇波动小,孕酮、绒毛膜促性腺激素呈下降趋势。雌二醇增加者在正常宫内孕组为91.3%,流产组为6.12%,宫外孕组为73.17%,差异有统计学意义(P <0.05)。结论在妊娠早期尚不能靠B 超确定妊娠结局前,检测血清雌二醇、孕酮、绒毛膜促性腺激素的动态变化对妊娠结局有预测价值。%Objective To investigate the dynamic changes of serum estradiol(E 2 ),progesterone(P)andhuman chorionic gonadotropin(HCG)on the predictive value of early pregnancy. Methods Choose 228 pregnant women after ovulation monitoring in natural cycle. According pregnancy outcomes,they were divided into 3 groups: normal pregnancy group,abortion,ectopic preg-nancy group. Set the ovulation day is 0 day,test the serum level of E 2 ,P and HCG on 15 ~ 17 day firstly,the second test after 2 ~3 days,third test after 7 days,and last test after 10 days. Compare the differences of hormonal on different intervals in three groups. Results E 2 and HCG had growing trends in normal pregnancy group,however P had small changes. E 2 and P decreased in abor-tion group,P was fluctuated,and the increase was significantly less than in the normal group. P and HCG were

  8. 体外受精-胚胎移植周期中注射人绒毛膜促性腺激素日患者血清孕酮水平与妊娠结局的相关性%Correlation between serum progesterone level at the day with human chorionic gonadotrophin administration and the outcome of pregnancy in in-vitro fertilization

    Institute of Scientific and Technical Information of China (English)

    奚秋萍; 蔡令波; 冯婷; 严正杰; 刘嘉茵; 冒韵东; 高彦; 丁卫; 王媁; 马翔; 刁飞扬; 黄洁; 钱晓乔

    2010-01-01

    Objective To investigate the relationship between serum progesterone level at the day with human chorionic gonadotrophin (hCG) administration and pregnant outcome from in in-vitro fertilization-embryo transfer(IVF-ET). Methods From Mar. 2002 to Apr. 2007, 786 cycles with serum progesterone measurement on the day of hCG administration for final oocyte maturation in IVF were analyzed retrospectively in Reproductive Medicine Center in First Affiliated Hospital of Nanjing Medical University.All stimulations were down-regulated with gronadotrophin release hormone agonist (GnRH-a) in both long protocols and short protocols before gonadotrophin stimulation. When the thresholds of serum progesterone were set at 5.5, 6.0,6.5,7.0,7.5,8.0,8.5 and 9.0 nmol/L, respectively. If the level of progesterone was less than the thresholds, those patients were in lower progesterone group, on the contrary, more than the threshold value, those patients were in higher progesterone group. The laboratory results and the clinical outcomes between all patients at lower and higher progesterone group at different thresholds value were analyzed. Results The rate of normal fertilization, quality embryos, successful implantation, chemical pregnancy, clinical pregnancy and live birth did not exhibit remarkable difference between patients with higher and lower serum progesterone level at multiple thresholds on the day of hCG administration in the 786 cycles (P >0.05). However, when the thresholds of serum progesterone were at 8.5 and 9.0 nmol/L, early abortion rates of 27.3% (3/11) and 3/7 in higher progesterone group were significantly higher than 8.8% (26/297) and 8.6% (26/301) in lower progesterone group (P0.05);以8.5及9.0 nmol/L为孕酮水平界值时,高孕酮水平患者的早期流产率分别为27.3%(3/11)和3/7,均高于低孕酮水平者[分别为8.8%(26/297)和8.6%(26/301)],差异均有统计学意义(P<0.05);以9.0 nmol/L为孕酮水平界值时,高孕酮水平患者的总流产率为3

  9. Gonadotrofina coriônica humana e hormônio liberador de gonadotrofina como indutores da reprodução do jundiá = Human chorionic gonadotropin and gonadotropin-releasing hormone as breeding inducers of jundiá

    Directory of Open Access Journals (Sweden)

    Paulo César Falanghe Carneiro

    2008-07-01

    Full Text Available Este trabalho foi realizado com o objetivo de avaliar parâmetros reprodutivos de machos e fêmeas de jundiá adultos após a aplicação do HCG (gonadotrofina coriônica humana e do GnRHa (hormônio liberador do hormônio gonadotrópico e compará-los àqueles obtidos quando usado o extrato hipofisário da carpa comum. Etapa 1 (Machos - quatro grupos, com oito machos cada, receberam os seguintes tratamentos: M1: sem hormônio; M2: extrato hipofisário 0,5 mg kg-1; M3: HCG 200 UI kg-1; M4: GnRHa+inibidor dopaminergético 0,1 glóbulo(Ovopel® kg-1. Etapa 2 (fêmeas – 36 fêmeas foram divididas em cinco grupos: F1: extrato hipofisário em duas aplicações, 0,5 e 5,0 mg kg-1; F2: HCG em duas aplicações, 200 e 400 UI kg-1; F3: HCG 400 UI kg-1 em doseúnica; F4: HCG 1000 UI kg-1 em dose única; F5: GnRHa+inibidor dopaminergético 1 glóbulo(Ovopel® kg-1. O uso do extrato hipofisário da carpa comum aumentou significativamente o volume de sêmen liberado e estimulou maior quantidade de fêmeas à liberar óvulos. O HCG e o GnRHa não apresentaram resultados positivos no tocante à reprodução induzida do jundiá, nas doses utilizadas neste estudo.In this study we analyzed reproductive parameters of males and females of jundiá induced by HCG (human chorionic gonadotropin and GnRHa (gonadotropin-releasing hormone, and compared the results tothose using carp pituitary extract. Stage 1 (Males - four groups with eight males each received one of the following treatments: M1: without hormone; M2: pituitary extract 0.5 mg kg-1; M3: HCG 200 UI kg-1; M4: GnRHa+dopamine receptor antagonist 0.1 pellet (Ovopel® kg-1. Stage 2 (Females – thirty-six females were separated in 5 groups: F1: pituitary extract in two applications, 0.5 and 5.0 mg kg-1; F2: HCG in two applications, 200 and 400 UI kg-1; F3: HCG 400 UI kg-1 in a single dose; F4: HCG 1000 UI kg-1 in a singledose; F5: GnRHa+dopamine receptor antagonist 1 pellet (Ovopel® kg-1. The pituitary extract

  10. Complicações materno-fetais da biópsia de vilo corial: experiência de um centro especializado do Nordeste do Brasil Fetal and maternal complications of chorionic villus sampling: results from a specialized center in the Northeast of Brazil

    Directory of Open Access Journals (Sweden)

    Antonio Carlos Vieira Lopes

    2007-07-01

    Full Text Available OBJETIVO: avaliar complicações maternas e fetais após realização de biópsia de vilo corial (BVC para diagnóstico pré-natal de alterações genéticas, na cidade de Salvador (BA. MÉTODOS: série de 958 gestantes de risco para cromossomopatias, submetidas à BVC realizada entre a nona e a 24ª semanas de gestação, por via transabdominal, utilizando agulha espinhal 18G 3½, guiada por ultra-sonografia, entre 1990 e 2006. As variáveis para a análise de complicações imediatas foram cólicas uterinas, hematoma subcoriônico, punção acidental da cavidade amniótica, dor no local da punção, amniorrexe, desconforto abdominal, bradicardia fetal e sangramento vaginal, e para complicações tardias, dor abdominal, sangramento vaginal, amniorrexe, infecção e abortamento espontâneo. Complicações obstétricas e fetais (parto prematuro, descolamento prematuro de placenta, placenta prévia e malformações anatômicas fetais foram também estudadas. Para análise estatística, utilizaram-se o chi² e o teste t de Student ou Mann-Whitney; o nível de significância foi 5%. RESULTADOS: a média de idade das gestantes foi 36,3±4,9 anos. Complicações imediatas foram encontradas em 182 (19% casos (cólica uterina em 14%, hematoma subcoriônico em 1,8% e punção amniótica acidental em 1,3% e tardias em 32 (3,3% casos (sangramento vaginal em 1,6%, dor abdominal em 1,4%, amniorrexe em 0,3% e aborto espontâneo em 1,6%. Não foi observado descolamento prematuro de placenta, placenta prévia ou malformação fetal. CONCLUSÕES: a BVC revelou-se procedimento simples e seguro. A BVC pode ser utilizada em gestantes que necessitam de diagnóstico pré-natal devido ao risco de anomalias genéticas.PURPOSE: to evaluate fetal maternal complications after chorionic villus sampling (CVS for prenatal diagnosis of genetic disorders in pregnant women of Salvador (BA, Brazil. METHODS: case-series study of 958 pregnancies with high risk for chromosomal

  11. 壳聚糖-HCG缓释制剂对鱼类生殖内分泌机能的影响%STUDIES ON THE EFFECTS OF SUSTAINED-RELEASE DELIVERY SYSTEMS OF CHITIN-HUMAN CHORIONIC GONADOTROPIN (CHITIN-HCG) ON REPRODUCTION ENDOCRINE FUNCTION OFGOLDFISH (CARASSIUS AURATUSL. VAR)

    Institute of Scientific and Technical Information of China (English)

    侯志帅; 温海深; 尹相菡

    2014-01-01

    以壳聚糖为原料制备壳聚糖-HCG 缓释制剂,进行激素埋植实验,间隔一定时间检测雌和雄鱼性腺发育、血清主要性激素含量和繁殖内分泌相关基因表达特征。结果表明:在埋植壳聚糖-HCG 缓释激素后,雌鱼性成熟系数(GSI)、血清睾酮(T)水平、血清雌二醇(E2)水平在6-30d内较对照组效果明显;雄鱼GSI仅在第6天显著高于对照组,血清T水平在第2、第14天高于对照组。血清E2水平在实验期间与对照组无显著差异。RT-PCR结果显示:性腺型P450芳香化酶(CYP19A)在性腺中表达丰富,心脏中最少。雌鱼性腺P450芳香化酶(CYP19A)基因mRNA相对表达量在第2、第6天显著高于对照组,雄鱼在第6天显著高于对照,雌鱼性腺雌激素受体(ERα)基因mRNA相对表达量在14-30d显著高于对照组,雄鱼在第6至第21天显著高于对照组。研究表明,壳聚糖-HCG 缓释制剂一次埋植后可在21d 内稳定持久地释放激素,对调节鱼类的生殖机能具有良好的促进效果。%With Sustained-release delivery systems of Chitin-Human Chorionic Gonadotropin (Chitin-HCG), at regular intervals after implantation of Chitin-HCG, we detected the gonadal developments, the levels of major sex hormones in serum and the relative expression levels of genes for reproduction endocrine function of female and male fish. The re-sults showed that implanted the Chitin-HCG, the gonado-somatic index (GSI), levels of testosterone (T) and estradiol (E2) of female fish were more effective than the controlled group from the 6th day to the 30th day. TheGSIof male fish was significantly higher than the controlled group only at the 6th day after implantation. The levels of testosterone (T) of male fish were significantly higher than the controlled group at the 2nd and the 14th day after implantation and the levels of estradiol (E2) of male fish showed no significant difference with the controlled group. With the RT

  12. The clinical significance of alpha-fetoprotein, human chorionic gonadotropin and unconjugated estriol in perinatal screening%甲胎蛋白、人绒毛膜促性腺激素及非结合雌三醇在产前筛查中的临床意义

    Institute of Scientific and Technical Information of China (English)

    庞春玉; 吴学礼

    2016-01-01

    目的:探讨甲胎蛋白、人绒毛膜促性腺激素及非结合雌三醇在产前筛查中的临床意义。方法:选取我院2014年12月至2015年6月孕周为15~20周的妊娠孕妇血清标本2070例,予以产前甲胎蛋白、人绒毛膜促性腺激素及非结合雌三醇的检测,并且结合孕妇的年龄、孕周、体质量等因素,运用配套的仪器产前筛查风险软件统计唐氏综合症、18–三体综合征及神经管缺陷的风险比例,予以高危孕妇做详细确诊,并依据随访与诊断结果比较高危组与低危组甲胎蛋白、人绒毛膜促性腺激素、非结合雌三醇水平,及出生缺陷阳性确诊率,产前筛查孕妇的年龄分布与出生缺陷的筛查阳性率。结果:高危组甲胎蛋白水平显著高于低危组[(28.74±1.35) vs.(6.93±1.20)μg/L],人绒毛膜促性腺激素水平显著高于低危组[(2.43±0.37) vs.(1.76±0.25) ng/L],非结合雌三醇水平明显低于低危组[(5.35±1.23) vs.(7.26±1.14)μg/L],两组比较差异明显(P35岁孕妇(26.24%);高危组孕妇出生缺陷总确诊阳性率(7.14%)明显高于低危孕妇(0.21%),两组比较差异显著。结论:甲胎蛋白、人绒毛膜促性腺激素及非结合雌三醇产前筛查能够有效筛查出生缺陷的概率,达到优生,值得推广运用。%Objective: To investigate the clinical signiifcance of alpha-fetoprotein (AFP), human chorionic gonadotropin (HCG) and unconjugated estriol (uE3) in perinatal screening.Methods: Selected 2 070 serum samples in pregnant women with gestational age of 15~20 weeks from December 2014 to June 2015 in our hospital, detected AFP, HCG and uE3 in prenatal testing, and combining with the age, gestational age, body weight and other factors, counted the riskratio of Down syndrome, trisomy 18 and the proportion of risk of neural tube defects by supporting instrument risk prenatal screening sotfware to diagnose in detail for high-risk women, and compared

  13. 血清瘦素、胱抑素C和人绒毛膜促性腺激素在妊娠高血压综合征检测中的意义%Study on clinical significance of serum leptin,cystatin C and human chorionic gonadotropin in patients with pregnancy - induced hyper- tension

    Institute of Scientific and Technical Information of China (English)

    刘炳刚; 李虹; 刘大庆

    2015-01-01

    目的:观察血清瘦素、胱抑素 C(CysC)和人绒毛膜促性腺激素(β- HCG)在妊娠高血压综合征检测中的意义。方法选取2011年1月至2014年6月收治的妊娠高血压患者150例为妊高症组;同期正常妊娠的患者60例为正常妊娠组;另选同期健康非妊娠妇女30例为健康对照组。观察健康对照组,正常妊娠组和妊高症组的瘦素、CysC 和β- HCG 水平,与妊高症严重程度的关系,各指标之间的相互关系。结果正常妊娠组和妊高症组的瘦素、CysC 和β-HCG 水平明显高于健康对照组( P ﹤0.01),而妊高症组明显高于正常妊娠组( P ﹤0.01)。随着妊高症病情严重程度的增加,瘦素,CysC 和β- HCG 水平出现明显的增高( P ﹤0.01)。妊高症患者瘦素水平与 CysC( r =0.673,P ﹤0.01)和β- HCG( r =0.838,P ﹤0.01)水平呈正相关,CysC 与β- HCG( r =0.792,P ﹤0.01)水平呈正相关。结论检测瘦素、CysC 和β- HCG 水平有助于对妊娠高血压患者的诊断和分级,其异常升高应引起临床的高度重视。%Objective To observe the clinical significance of serum leptin,cystatin C(CysC)and human chorionic gonadotropin(β -HCG)in patients with pregnancy - induced hypertension. Methods A total of 150 patients with pregnancy - induced hypertension during January 2011 to June 2014 were selected as pregnancy - induced hypertension group;60 patients with normal pregnancy at the same period were allocated in normal pregnancy group,and 30 healthy women without pregnancy were selected as healthy control group. Levels of leptin,CysC and β - HCG were examined among pregnancy - induced hypertension group,normal pregnancy group and healthy control group,the relationship among levels of leptin,CysC and β - HCG and the severity of patients with pregnancy - induced hypertension were observed,the correlation between each marker had been evaluated. Results Levels of leptin

  14. Determination of serum β-human chorionic gonadotropin, progesterone, carbohydrate antigen 125,creatinekinase and creatinekinase/progesterone for early diagnosis of ectopic pregnancy%血清人绒毛膜促性腺激素β亚基、孕酮、糖类抗原125、肌酸激酶及肌酸激酶/孕酮值在异位妊娠早期诊断中的应用价值

    Institute of Scientific and Technical Information of China (English)

    刘金花; 徐吟亚; 姜波玲

    2011-01-01

    Objective To explore the value of serum p-human chorionic gonadotropin (p-HCG), progesterone( P), carbohydrate antigen 125 (CA125) ,creatinekinase(CK) and creatinekinase/progesterone (CK/P) for early diagnosis of ec-topic pregnancy (EP). Methods Serum contents of p-HCG,P,CA125 (with chemiluminescent immunoassay) and CK (with enzymatic kinetic method) were determined in 72 patients with suspected early ectopic pregnancy (observation group) and 72 normal pregnant women (control group) as to analyze the value of serum p-HCG combined with P,CA125,CK and CK/P for early diagnosis of EP. Results The serum levels of p-HCG, P,CA125 in the observation group were significantly lower than those in the control group (P 0. 05) ; the serum level of CK/P in the observation group was significantly higher than that in the control group (P<0.01).The specificity and accuracy of serum p-HCG combined with P,CA125,CK/P for early diagnosis of EP were respectively 88.9% and 91.7%. Conclusion Combined determination of p-HCG,P.CA125 and CK/P may contribute to the early diagnosis of EP and help to provide a valuable clinical diagnosis.%目的 探讨血清人绒毛膜促性腺激素β亚基(β-HCG)、孕酮(P)、糖类抗原125 (CA125)、肌酸激酶(CK)及CK/P值在异位妊娠早期诊断中的应用价值.方法 选择临床可疑异位妊娠患者72例作为观察组,正常宫内妊娠72例作为对照组.于治疗前采用化学发光免疫分析法测定血清β-HCG、P及CA125水平,采用酶动力学方法检测CK水平,在血清β-HCG基础上分析多项指标联合检测对早期异位妊娠的诊断价值.结果 观察组血清β-HCG、P及CA125水平均显著低于对照组(P<0.01);观察组的CK较对照组略有升高,但差别无统计学意义(P>0.05);观察组的血清CK/P值显著高于对照组(P<0.01).血清β-HCG、P、CA125及CK/P值4项指标联合检测可将诊断特异度、准确性分别提高至88.9%、91.7%.结论 血清β-HCG、P、CA125及CK/P值

  15. Effects of equine chorionic gonadotropin, human chorionic gonadotropin, and laparoscopic artificial insemination on embryo, endocrine, and luteal characteristics in the domestic cat.

    Science.gov (United States)

    Roth, T L; Wolfe, B A; Long, J A; Howard, J G; Wildt, D E

    1997-07-01

    The effects of gonadotropin treatment and laparoscopic artificial insemination (AI) on embryo quality, serum progesterone and estradiol concentrations, and luteal progesterone content were examined in the domestic cat. These data were compared to similar historical data reported for naturally estrual, mated queens. All queens in this study (n = 32) were treated with eCG followed by 1) natural breeding (eCG-NB), 2) NB and hCG (eCG-NB-hCG), 3) NB and a sham AI procedure (eCG-NB-sham AI), or 4) hCG and actual AI (eCG-hCG-AI). Queens ovulating in response to treatment were ovariohysterectomized, and oviducts and uteri were flushed to collect embryos. Ovarian structures were recorded, corpora lutea (CL) were excised and evaluated for progesterone content, and serum was analyzed for estradiol-17beta and progesterone. Follicle and CL numbers ranged from 0 to 28 and 2 to 42 per cat, respectively, and treatment means did not differ (p > or = 0.05) among groups. Embryos were recovered from oviducts and uterine horns in all treatment groups, and recovery ranged from 60-96%. Mean embryo number per queen ranged from 8.2 +/- 2.6 to 23.2 +/- 3.8 and did not differ (p > or = 0.05) among groups. However, the proportions of unfertilized oocytes were greater (p or = 0.05) among treatments. Compared to historical controls (naturally estrual, mated queens), eCG-NB queens produced > 4 times as many good-quality embryos and blastocysts. Similarly, eCG-hCG-AI-treated queens produced > 4 times the number of oocytes and embryos, although a high proportion of these were poor quality and did not develop to blastocysts. Together, these results indicate that queens treated with eCG are capable of consistently producing many good-quality embryos, at least half of which develop to blastocysts in culture. These data support the use of eCG in felids and suggest that other factors are responsible for reduced pregnancy success and small litter sizes following assisted reproduction. PMID:9209095

  16. 南昌地区妊娠14~20周妇女血清甲胎蛋白和游离绒毛膜促性腺激素的MOM值测定及临床应用%Detection and clinical application of MOM values of serum alpha-fetoprotein and free human chorionic gonadotropin in pregnant women during 14~20 gestational weeks in Nanchang region

    Institute of Scientific and Technical Information of China (English)

    赖华; 刘艳秋; 刘淮

    2011-01-01

    目的:运用计算出的AFP及F-βHCG的MOM值与目前采用的MOM值对孕妇进行胎儿神经管缺陷畸形及唐氏综合征筛查,期待能更有效地筛查出南昌地区胎儿神经管缺陷及唐氏综合征高危孕妇.方法:依据2005~2007年到江西省妇幼保健院产前诊断门诊就诊的孕妇各孕周AFP、F-βHCG的中位数,计算出AFP、F-βHCG的MOM值,用计算出的MOM值(研究组)和目前采用的MOM值(对照组)分别对2008年来该院产前诊断门诊就诊的孕妇进行胎儿神经管缺陷畸形及唐氏综合征筛查,对筛查出的高危孕妇进行羊水检测和随访,比较两种MOM值对胎儿神经管缺陷畸形和唐氏综合征筛查的阳性率、假阴性率及假阳性率.结果:神经管缺陷畸形、唐氏综合征阳性率研究组高于对照组,具有统计学意义(P<0.01);假阴性率研究组与对照组比较,结果无统计学意义;假阳性率研究组较对照组降低,结果具有统计学意义,(神经管缺陷畸形P<0.05,唐氏综合征P<0.01).结论:应用本地区MOM值进行神经管缺陷畸形及唐氏综合征筛查,能更有效地筛查出本地区的神经管缺陷畸形及唐氏综合征胎儿.%Objective: To screen fetal neural tube defect and Down's syndrome by calculated MOM values and values currently used of serum alpha - fetoprotein (AFP) and free β human chorionic gonadotropin (F - β HCG) in pregnant women, in order to screen out the high risk pregnant women of fetal neural tube defect and Down's syndrome in Nanchang region.Methods: The MOM values of AFP and F - β HCG were calculated according to the medians of AFP and F - β HCG in pregnant women of different gostational weeks who visited the prenatal diagnosis outpatient of the hospital from 2005 to 2007, then the calculated MOM values ( study group) and the MOM values currently used ( control group) were used to screen fetal neural tube defect and Down's syndrome among the pregnant women who visited the prenatal

  17. The relationship between the single level of serum human chorionic gonadotropin(HCG) at 14~15th day after embryo transfer and the multiple-pregnancy, the gender of fetal, the clinic abortion%胚胎移植术14~16天后单次血HCG值与多胎,胎儿性别和临床流产之关系

    Institute of Scientific and Technical Information of China (English)

    贾婵维; 王树玉; 马延敏; 兰永连; 周丽颖; 刘英; 张军; 余兰

    2011-01-01

    Objective: To determine the relationship between serum human chorionic gonadotrop (HCG) at 14 days - 16 days after embryo transfer and pregnancy outcome in long run. Methods: The pregnancy outcomes of 329 cases whose gestational sacs were detected and the serum HCG levels of 14 days - 16 days after fresh cycle IVF/ICSI - ET were retrospectively analyzed. Among 329 cases, there were 145 case detected the serum HCG level at the 14th day after embryo transfer, 102 cases detected the serum HCG level at the 15th day after embryo transfer and 82 cases at the 16th day after embryo transfer. According to the day of detecting serum HCG. We divided the 329 cases into four groups by their outcomes: multiple pregnancy, singleton female infant, singleton male infant, clinical abortion. And according the day of detecting serum HCG we compared their levels of serum HCG after embryo transfer using One - Way ANOVA test. Results: 1. The levels of serum HCG went down according to multiple pregnancies, singleton female infants, singleton male infants, clinical abortions. There was the significant difference between multiple pregnancies and other groups. 2. At the 14th day after embryo - transfer, there was the significant difference of the serum HCG levels between singleton female infants and singleton male infants, clinical abortions but there was no significant difference between singleton male infants and clinical abortions. 3. At 15th and 16th day after embryo -transfer, there was the significant difference of the serum HCG levels between singleton female infants and clinical abortions but no significant difference between singleton male infants and clinical abortions.There was also no significant difference between singleton female infants and singleton male infants. Conclusion: 1. The levels of serum HCG at the 14 - 16th day after embryo - transfer were correlated with their pregnancy outcome. The mean levels of serum HCG step down according multiple pregnancy, singleton female

  18. 77 FR 55413 - New Animal Drugs; Chorionic Gonadotropin; Naloxone; Oxymorphone; Oxytocin

    Science.gov (United States)

    2012-09-10

    ... Gonadotropin; Naloxone; Oxymorphone; Oxytocin AGENCY: Food and Drug Administration, HHS. ACTION: Final rule.... Painters Dr., Chadds Ford, PA 19317. 046-822 VETOCIN (oxytocin) United Vaccines, A Harlan...

  19. Myth vs. Fact: The Human Chorionic Gonadotropin (hCG) Diet

    Science.gov (United States)

    ... 500 calories per day on the diet. Experts worry that this diet puts people’s health at risk. In general, women need 1,600 to 2,400 calories per day and men need 2,000 to 3,000 calories per day to maintain a healthy weight. Following a healthy diet when eating only ...

  20. Development of a radioreceptor assay for human chorion gonadotropin: Application in normal and pathological pregnancies

    International Nuclear Information System (INIS)

    Rats testes were homogenised, and the binding capacity of several dilutions of these were tested with iodine125-labelled human choriongonadotropin. Investigations about binding over a period of 36 hrs. with 3 different temperatures, inhibition tests and cross reaction analyses for determining the specificity were carried out. 2 assay systems could be developed. The highly sensitive assay was applied at early pregnancy, at suspected disturbed or ectopic gravidity and allowed to measure the hCG-serum concentration above the physiological basal secretion of hLH. The less sensitive assay was used for measuring hCG in later stages of pregnancy, chorionepitheliomas and other hCG producing tumours. With the highly sensitive and specific assay, hCG was determinable 8 to 10 days post conceptionem. (orig.)

  1. Prenatal Genetic Diagnosis in 481 Amniocentesis, Chorion Villi Sample and Cordocentesis Specimens

    Directory of Open Access Journals (Sweden)

    Turgay Budak

    2007-01-01

    Full Text Available In this study, we evaluated a total of 481 amniocentesis , cordocentesis and corion villi sample specimens from patients who were referred to the Prenatal Diagnostic Laboratory of Department of Medical Biology and Genetics Department of Medical Faculty of University of Dicle, between 1999 and 2001. A total of 24 specimens were found cytogenetically abnormal, of which 11 were trisomy 21 ( Down Syndrome, two were Down Syndrome with Robertsonian type of translocation between chromosome 14 and 21, one was mosaic Down Syndrome , one was balanced translocated chromosome carrier, two were Turner Syndrome, one was triple X syndrome, two were triploidy, one was partial trisomy 3, one was derivative chromosome, one was nonrepetitive numerical and structural abnormality, and one was marker chromosome. Unfortunately, we could not have results in 15 of culture samples. There were no false positive and false negative results.

  2. ABNORMAL DIFFERENTIATION OF THE CHORION AND ITS RELEVANCE TO THE DEVELOPMENT OF THE CONCEPTUS

    Directory of Open Access Journals (Sweden)

    DD Farhud; H Sadighi ; M.R. Mohammad Hassani ; A. Samavat; R. Zakizadeh ; Z. Yazdani

    1996-08-01

    Full Text Available Thalassemias, because of climatic, geographic and ecological conditions, are the most common among the genetically endemic in Iran, especially m provinces adjacent to the Caspian Sea and the Persian Gulf. There are over 14,000 cases of thalassemia major reported in Iran. Data, collected by the Iranian Ministry of Health, and analyzed at the Department of Human Genetics, School of Public Health, Tehran University of Medical Sciences, showed relative distribution of 3194 patients referring for the iron chelating drug, disferal, in 24 provinces in Iran. 3304 cases were studied for sex and age groups. Higher percentages and sex ratios were observed in each age group and further clarified as the age increased. 3386 cases were considered for ABO and Rh blood groups. Significant high incidence of group 0 (41.228% was followed by groups A (29.090% and B (23.2 13%, and group AB with the lowest (6.467%. A significant low incidence of Rh negative was also observed (6.852%.

  3. Midtrimester serum B-subunit human chorionic gonadotropin levels and the subsequent development of preeclampsia

    Directory of Open Access Journals (Sweden)

    Moghaddami Tabrizi N

    2001-09-01

    Full Text Available The pre-eclampsia/eclampsia is one of the commonest conditions peculiar to pregnancy, to describe the occurrence of hypertension, proteinuria and edema in pregnancy. It is about seven times more frequent in first gestation and uncommon before 20 week's gestation. Serum BhCG levels were prospectively determined in 260 midtrimester singleton pregnancies where admitted from October 1999 until September 2000 at Mirza Kouchak Khan hospital, prenatal clinic. Obstetric chart review was undertaken after delivery to identify cases in which pre eclampsia developed. The median maternal age was 26.0 years, and the median gestational age at the time of blood collection was 19.0 weeks. Of these, 17 cases (6.5 percent had pre eclampsia. The median level of the BhCG was 35060.5 mlu/ml in those with pre eclampsia, whereas that in those without pre eclampsia was 33755.1 mlu/ml. The difference was not significant (P=0.28. Although elevated midtrimester serum BhCG levels in clinically normal patients was reported in severaly pre eclampsia women, this is not a good test for early detection of mild pre eclampsia.

  4. Structural and functional analysis of rare missense mutations in human chorionic gonadotrophin β-subunit

    DEFF Research Database (Denmark)

    Nagirnaja, Liina; Venclovas, Česlovas; Rull, Kristiina;

    2012-01-01

    CG by applying a combination of in silico (sequence and structure analysis, molecular dynamics) and in vitro (co-immunoprecipitation, immuno- and bioassays) approaches. The carrier status of each mutation was determined for 1086 North-Europeans [655 patients with recurrent miscarriage (RM)/431 healthy controls......) and was inherited by two of seven studied live born children. The mutation caused ~50% of secreted β-subunits to acquire an alternative conformation, but did not affect its biological activity. For the CGB8 p.Arg8Trp (rs72556341) substitution, the applied in vitro methods revealed no alterations in the assembly...... of intact hCG as also supported by an in silico analysis. In summary, the accumulated data indicate that only mutations with neutral or mild functional consequences might be tolerated in the major hCGβ genes CGB5 and CGB8....

  5. THE EFFECTS OF HUMAN CHORIONIC GONADOTROPIN AND TYROSINE PROTEIN KINASE ON THE GROWTHOF HYBRIDOMA CELLS

    Institute of Scientific and Technical Information of China (English)

    HANShou-Wei; LIUShah-Ling; CAOZe-Yi; CHENMan-Ling

    1989-01-01

    In recent years, the production and development of receptor monoclonal antibodies (McAB) have been attentively studied. Wc observed the effects of human ehorionicgonadotropin (HCG) and tyrosinc protein kinase (TPK) on the growth of two hybridoma

  6. Graded hyperthyroidism and serum human chorionic gonadotropin concentration in patients with trophoblastic disease

    International Nuclear Information System (INIS)

    Serum thyroid hormone and basal and post TRH stimulated levels of TSH were measured in 48 female subjects of mean age 29.3 ± 9.2 and mean gravida 2.9 ± 2.6 with trophoblastic disease (TD), both benign and malignant. Normal pregnant women (n=21) served as controls. Twenty-five patients showed a normal response to TRH (Group i) while the rest (Group ii) had subnormal response while thyroid hormone levels were increased. Two subgroups iiA and iiB were formed within Group ii on the basis of the free T4 levels (measured by equilibrium dialysis) falling below or above the 25th percentile. hCG levels were higher in Group ii than in Group i and a stepwise significant increase in the mean level of this hormone was observed in Group i to iiA and iiB. Significant correlation between hCG levels and those of thyroxine, free thyroxine, and triiodothyronine were found in TD patients as a whole, but not within the different subgroups. Clinical signs were minimal, with proximal muscle weakness and fine finger tumours observed in 10 patients in Group iiB. The study shows that the incidence of biochemical hyperthyroidism is higher than was reported before sensitive methods for TSH measurement were available, and postulates that increased hCG concentrations in themselves and/or abnormal metabolic variants of hCG produced by trophoblastic tumours may act as thyroid stimulators in this condition. 64 refs, 5 figs, 4 tabs

  7. Correlation between second trimester beta human chorionic gonadotropin levels and pregnancy outcome in high risk group

    Directory of Open Access Journals (Sweden)

    Praveen Sharma

    2016-07-01

    Conclusions: Women with bad obstetric history and unexplained elevation of serum beta hCG during second trimester are at an increased risk for PIH and IUGR. [Int J Reprod Contracept Obstet Gynecol 2016; 5(7.000: 2358-2361

  8. Insulin stimulates synthesis and release of human chorionic gonadotropin by choriocarcinoma cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Ren, S.G.; Braunstein, G.D. (Univ. of California School of Medicine, Los Angeles (USA))

    1991-03-01

    Recent studies have shown that insulin regulates placental lactogen, progesterone, and estrogen production from human trophoblast cells. This study was performed to examine whether insulin also regulates the production of hCG by this type of cell. After 24-36 h of preincubation, JEG-3 and JAR cells (2-3 x 10(5) cells/ml.well) or human term trophoblast cells (1 x 10(6) cells/ml.well) were exposed to the test hormone in serum-free Dulbecco's Modified Eagle's Medium for 24-96 h. Secretion of hCG from JEG-3 cells was stimulated by human insulin, human proinsulin, or porcine insulin in a dose-dependent manner, with lowest effective doses of 6.7, 96, and 53 mg/L, respectively. Time-course studies showed that hCG secretion peaked at 72-96 h with insulin exposure; in contrast, no decernable peak was seen without insulin in serum-free media. Exposure of JEG-3 cells for 24 h to 209 mg/liter insulin stimulated hCG synthesis, with 40 +/- 3% more immunoreactive intracellular hCG (P less than 0.05). Cells grown in the presence of insulin and (35S)methionine had 47 +/- 21% more labeled intracellular hCG and 56 +/- 13% more immunoprecipitable (35S)methionine-hCG secreted into the medium than the control cultures (P less than 0.05). During this time period, human placental lactogen release and total trichloroacetice acid-precipitable (35S)methionine protein were not increased. The insulin-induced stimulation of hCG synthesis was inhibited by cycloheximide. Additionally, insulin did not significantly affect total intracellular protein during 24-96 h of incubation. Insulin also increased hCG release from JAR cells, but not from human term trophoblast cells. A mouse monoclonal antibody to the IGF-I receptor inhibited the stimulation of insulin in JEG-3 cells.

  9. Insulin stimulates synthesis and release of human chorionic gonadotropin by choriocarcinoma cell lines

    International Nuclear Information System (INIS)

    Recent studies have shown that insulin regulates placental lactogen, progesterone, and estrogen production from human trophoblast cells. This study was performed to examine whether insulin also regulates the production of hCG by this type of cell. After 24-36 h of preincubation, JEG-3 and JAR cells (2-3 x 10(5) cells/ml.well) or human term trophoblast cells (1 x 10(6) cells/ml.well) were exposed to the test hormone in serum-free Dulbecco's Modified Eagle's Medium for 24-96 h. Secretion of hCG from JEG-3 cells was stimulated by human insulin, human proinsulin, or porcine insulin in a dose-dependent manner, with lowest effective doses of 6.7, 96, and 53 mg/L, respectively. Time-course studies showed that hCG secretion peaked at 72-96 h with insulin exposure; in contrast, no decernable peak was seen without insulin in serum-free media. Exposure of JEG-3 cells for 24 h to 209 mg/liter insulin stimulated hCG synthesis, with 40 +/- 3% more immunoreactive intracellular hCG (P less than 0.05). Cells grown in the presence of insulin and [35S]methionine had 47 +/- 21% more labeled intracellular hCG and 56 +/- 13% more immunoprecipitable [35S]methionine-hCG secreted into the medium than the control cultures (P less than 0.05). During this time period, human placental lactogen release and total trichloroacetice acid-precipitable [35S]methionine protein were not increased. The insulin-induced stimulation of hCG synthesis was inhibited by cycloheximide. Additionally, insulin did not significantly affect total intracellular protein during 24-96 h of incubation. Insulin also increased hCG release from JAR cells, but not from human term trophoblast cells. A mouse monoclonal antibody to the IGF-I receptor inhibited the stimulation of insulin in JEG-3 cells

  10. The Effect of Human Chorionic Gonadotropin Treatment Before Testicular Sperm Extraction in Non-Obstructive Azoospermia

    Directory of Open Access Journals (Sweden)

    Ümit Gul

    2014-12-01

    Full Text Available Aim: To investigate our experience on empirical hCG treatment of patients with idiopathic non-obstructive azoospermia (NOA. Material and Method: hCG group consisted of 34 patients who were empirically treated with hCG despite normal serum FSH and LH levels and normal testicular volumes. hCG was administered as 2500 IU twice weekly subcutaneous injections for 10 to 14 weeks prior to testicular sperm extraction (TESE. Control group consisted of 49 age and spouse age matched patients who underwent TESE in the same time period. Sperm retrieval rate (SRR, and follicle stimulating hormone (FSH, lutenizing hormone (LH and testosterone levels, volume of testicles, fertilization rate (FR, implantation rate (IR, pregnancy rate (PR, live birth rate (LBR and cancel rate (CR and surgical technique were compared between the two groups. Results: Conventional technique was used in 14 of the 17 patients (82.3% with successful sperm retrieval in the hCG group, and 18 of the 28 patients (64.3% in the control group (p=0.170. There were no differences between groups in terms of SRR (p=0.338. There were no significant differences in patient age, mean infertility period, mean values of FSH, LH, testosterone, estradiol levels, and testis volume between the two groups (p>0.05. There were no statistically significant differences for FR, IR, PR, LBR between the two groups (p>0.05. Discussion: Empirical hCG treatment in patients with idiopathic NOA did not result in improved SRR. hCG treatment did not have any effect on the success of ICSI.

  11. Expression of the Inducible Nitric Oxide Synthase Isoform in Chorionic Villi in the Early Spontaneous Abortion

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    To investigate the relationship between inducible nitric oxide synthase (iNOS) and the early spontaneous abortion. , in situ hybridization and immunohistochemistry were used to detect the expression of iNOS in trophoblasts in the early pregnancy with and without spontaneous abortion (group Ⅰ and group Ⅱ ). By light microscopy and computer color magic image analysis system (CMIAS), light density (D) and the positive cell number per statistic square (N/S) in situ hybridization were used to analyze the positive cell index, while total positive cells (N) and the positive unit (Pu) were used in immunohistochemistry. By in situ hybridization, D and N/S in trophoblasts were 0. 35±0. 028, 0. 07±0. 011 respectively in group Ⅰ and 0. 18±0. 016,0. 015±0. 003 in group Ⅱ . In terms of immunohistochemical staining, N and Pu were 0. 058±±0. 007, 11. 94±2. 01 in group Ⅰ and 0. 013±0. 009, 1. 08±0. 35 in group Ⅱ in trophoblasts. Significant differences existed between two groups. It is concluded that the higher nitric oxide produced by the higher expression of iNOS in trophoblasts might play an important role in the early spontaneous abortion.

  12. Serum inhibin B, FSH, LH and testosterone levels before and after human chorionic gonadotropin stimulation in prepubertal boys with cryptorchidism

    DEFF Research Database (Denmark)

    Christiansen, P; Andersson, A-M; Skakkebaek, N E;

    2002-01-01

    Several studies have indicated that cryptorchidism is associated with degenerative changes in both Sertoli cells and germ cells. The gonadal peptide hormone inhibin B reflects Sertoli cell function. Low inhibin B levels are found in a large portion of formerly cryptorchid men who show compromised...... seminiferous tubule function. It is not known if inhibin B can be used to demonstrate early damage of seminiferous tubules in prepubertal boys with cryptorchidism....

  13. Optimisation of an oviposition protocol employing human chorionic and pregnant mare serum gonadotropins in the Barred Frog Mixophyes fasciolatus (Myobatrachidae)

    OpenAIRE

    Clulow John; Clulow Simon; Guo Jitong; French Andrew J; Mahony Michael J; Archer Michael

    2012-01-01

    Abstract Background Protocols for the hormonal induction of ovulation and oviposition are essential tools for managing threatened amphibians with assisted reproduction, but responses vary greatly between species and even broad taxon groups. Consequently, it is necessary to assess effectiveness of such protocols in representative species when new taxa become targets for induction. The threatened genus Mixophyes (family Myobatrachidae) has amongst the highest proportion of endangered species of...

  14. The Effects of Fetal Gender on Serum Human Chorionic Gonadotropin and Testosterone in Normotensive and Preeclamptic Pregnancies

    Directory of Open Access Journals (Sweden)

    Nahid Lorzadeh

    2012-01-01

    gender and significantly higher in mlae-bearing than in female-bearing pregnancies. Conclusion. According to our results, there is a correlation between maternal serum hCG and testosterone levels and preeclampsia. Therefore these tests can be used as routine during 30–38 weeks of gestation. High maternal serum concentrations of these markers can predict preeclampsia.

  15. Induction of ovulation in quarter horse mares through the use of deslorelin acetate and human chorionic gonadotrophin (hCG)

    OpenAIRE

    Tatiana Figueiredo; Roman Paiva; Luiz Ernandes Kozicki; Fernanda Kaercher; Romildo Romualdo Weiss; Ivo Walter dos Santos; Priscilla Ricabone Muradas

    2011-01-01

    The aim this study was to compare two protocols of induction for ovulation by desloreline acetate and hCG in Quarter Horse mares. The choice of the animals was based on the observations by the estrus, by rectal palpation of the ovaries and by ultrassonography of the follicular dynamics. After estrus detection and follicle control, the measurement of the follicles and the classification of uterus were carried out. The animals that had dominant follicle (diameter more than 35 mm) and swollen ut...

  16. Induction of ovulation in quarter horse mares through the use of deslorelin acetate and human chorionic gonadotrophin (hCG

    Directory of Open Access Journals (Sweden)

    Tatiana Figueiredo

    2011-06-01

    Full Text Available The aim this study was to compare two protocols of induction for ovulation by desloreline acetate and hCG in Quarter Horse mares. The choice of the animals was based on the observations by the estrus, by rectal palpation of the ovaries and by ultrassonography of the follicular dynamics. After estrus detection and follicle control, the measurement of the follicles and the classification of uterus were carried out. The animals that had dominant follicle (diameter more than 35 mm and swollen uterus were used. In these conditions, the mares received hCG or desloreline acetate. Once ovulation occurred, the artificial insemination was carried. Two groups were performed: G1 (20 animals received 1.5 mg desloreline acetate and G2 (20 animals received 1700 IU of hCG. Following 6h intervals, the control follicular was performed by ultrasonography. The follicular average diameter was 42.6 cm for the groups and set up a score of 0 to 3 of uterine edema displayed by the device as well as the time of ovulation. In conclusion, the desloreline acetate showed better performance than hCG, because the ovulation was induced in less time (nine hours than hCG (p<0.05.The pregnancy rate was 80 and 75 %, respectively in G1 and G2.

  17. Semen quality in men with disseminated testicular cancer : relation with human chorionic gonadotropin beta-subunit and pituitary gonadal hormones

    NARCIS (Netherlands)

    de Bruin, Daphne; de Jong, Igle J.; Arts, Eugene G. J. M.; Nuver, Janine; Dullaart, Robin P. F.; Sluiter, Willem J.; Hoekstra, Harald J.; Sleijfer, Dirk T.; Gietema, Jourik A.

    2009-01-01

    Objective: To compare the semen quality and hormonal status between patients with testicular cancer and normal versus increased serum levels of beta-hCG. Design: Retrospective study. Setting: Academic research environment. Patient(s): All 203 patients with testicular cancer who required chemotherapy

  18. High-resolution Elemental Mapping of Human Placental Chorionic Villi Using Synchrotron X-ray Fluorescence Spectroscopy

    Energy Technology Data Exchange (ETDEWEB)

    Punshon, Tracy; Chen, Si; Finney, Lydia; Howard, Louisa; Jackson, Brian P.; Karagas, Margaret R.; Ornvold, Kim

    2015-09-01

    The placenta is the organ that mediates transport of nutrients and waste materials between mother and fetus. Synchrotron X-ray fluorescence (SXRF) microanalysis is a tool for imaging the distribution and quantity of elements in biological tissue, which can be used to study metal transport across biological membranes. Our aims were to pilot placental biopsy specimen preparation techniques that could be integrated into an ongoing epidemiology birth cohort study without harming rates of sample acquisition. We studied the effects of fixative (formalin or glutaraldehyde) and storage duration (30 days or immediate processing) on metal distribution and abundance and investigated a thaw-fixation protocol for archived specimens stored at -80 A degrees C. We measured fixative elemental composition with and without a placental biopsy via inductively coupled plasma mass spectrometry (ICP-MS) to quantify fixative-induced elemental changes. Formalin-fixed specimens showed hemolysis of erythrocytes. The glutaraldehyde-paraformaldehyde solution in HEPES buffer (GTA-HEPES) had superior anatomical preservation, avoided hemolysis, and minimized elemental loss, although some cross-linking of exogenous Zn was evident. Elemental loss from tissue stored in fixative for 1 month showed variable losses (a parts per thousand 40 % with GTA-HEPES), suggesting storage duration be controlled for. Thawing of tissue held at -80 A degrees C in a GTA-HEPES solution provided high-quality visual images and elemental images

  19. Association of testicular non-Hodgkin's lymphomas with elevated serum levels of human chorionic gonadotropin-like material

    DEFF Research Database (Denmark)

    Møller, Michael Boe

    1996-01-01

    in nontesticular non-germ cell tumors including non-Hodgkin's lymphomas (NHL) as well. It has never been investigated whether testicular NHL is also associated with elevated S-hCG-1. In the present study the relationship of testicular NHL with increased S-hCG-1 was investigated. In the Danish population-based NHL...... registry, LYFO registry, 12 cases with testicular involvement of the lymphoma at the time of diagnosis and that had S-hCG-1 measured prior to treatment were identified, and cases with elevated S-hCG-1 were analyzed clinicopathologically. Of these, 2 patients had elevated levels. Both cases were high...

  20. Serum insulin-like factor 3 is highly correlated with intratesticular testosterone in normal men with acute, experimental gonadotropin deficiency stimulated with low-dose human chorionic gonadotropin

    DEFF Research Database (Denmark)

    Roth, Mara Y; Lin, Kat; Bay, Katrine;

    2013-01-01

    To study the potential role for using serum biomarkers, including insulin-like factor 3 (INSL3), 17α-hydroxyprogesterone, antimüllerian hormone, and inhibin B, as correlates of intratesticular T (IT-T) concentrations in men....

  1. Triggering of final oocyte maturation with gonadotropin-releasing hormone agonist or human chorionic gonadotropin. Live birth after frozen-thawed embryo replacement cycles

    DEFF Research Database (Denmark)

    Griesinger, Georg; Kolibianakis, E M; Papanikolaou, E G;

    2007-01-01

    . PATIENT(S): Patients under observation previously had been recruited into two concurrently performed, independent, randomized controlled trials (comparing hCG with GnRH-agonist for triggering final oocyte maturation in GnRH-antagonist multiple-dose protocols in normal responder patients) encompassing...

  2. Early and late half-life of human chorionic gonadotropin as a predictor of persistent trophoblast after laparoscopic conservative surgery for an ectopic pregnancy

    OpenAIRE

    Billieux, Marie-Hélène

    2003-01-01

    Cette étude a été effectuée dans le but de déterminer si le calcul de la 1ère ou 2ème demi-vie de l'hormone chorionogonadotrophique (hCG) permettait d'identifier la présence de trophoblaste persistant après traitement chirurgical conservateur de grossesse extra-utérine par laparoscopie. Après un abortus ou un accouchement, l'hCG décroît, dans la circulation sanguine, selon une cinétique de 1er ordre, caractérisée par une disparition rapide (1ère demi-vie) puis plus lente (2ème demi-vie). Entr...

  3. Early and late half-life of human chorionic gonadotropin as a predictor of persistent trophoblast after laparoscopic conservative surgery for an ectopic pregnancy

    OpenAIRE

    Billieux, Marie-Hélène; Bischof, Paul

    2005-01-01

    Cette étude a été effectuée dans le but de déterminer si le calcul de la 1ère ou 2ème demi-vie de l'hormone chorionogonadotrophique (hCG) permettait d'identifier la présence de trophoblaste persistant après traitement chirurgical conservateur de grossesse extra-utérine par laparoscopie. Après un abortus ou un accouchement, l'hCG décroît, dans la circulation sanguine, selon une cinétique de 1er ordre, caractérisée par une disparition rapide (1ère demi-vie) puis plus lente (2ème demi-vie). Entr...

  4. 胎盘闭塞性动脉内膜炎致死胎一例%Stillbirth due to obliterative endarteritis of chorionic villi: report of a case

    Institute of Scientific and Technical Information of China (English)

    廖秋林

    2009-01-01

    @@ 患者女,27岁.因"停经36周,胎死宫内"于2008年9月收住院.患者平素月经规律,孕60~+d出现恶心、呕吐等早孕反应,孕4~+个月后始自觉胎动,孕2个月余在外院查单纯疱疹病毒型-IgG(+).

  5. hCGβ在乳酸杆菌中的分泌性表达%Expression of Human Chorionic Gonadotropin Beta-Subunit in Lactobacillus Casei

    Institute of Scientific and Technical Information of China (English)

    王红梅; 李大金; 姜红; 袁正宏

    2003-01-01

    目的:探索乳酸杆菌携带表达hCGβ避孕疫苗的抗生育作用.方法:用PCR从短小乳酸杆菌染色体中扩增得到S-层蛋白的信号肽序列,将其与hCGβ基因连接后克隆到乳酸杆菌整合型表达载体pIlac的lac启动子下游,得到质粒pIlac hCGβ,电穿孔转化干酪氏乳酸杆菌Lb. casei CECT5276.挑选转化后的耐药菌落,在含红霉素培养基中培养,抽提其染色体DNA,PCR及测序鉴定证实hCGβ基因整合到Lb. casei的基因组中.hCG放免检测上清及细胞裂解液中的hCGβ.结果:hCGβ基因已经整合到Lb. casei的基因组中;细菌产生的目的蛋白约有3/4分泌进入培养上清,而加入诱导剂乳糖21 h后,上清中的蛋白浓度达到峰值,为440 mIU/mL.重组菌在无选择压力条件下培养50代后仍可分泌hCGβ.结论:hCGβ在乳酸杆菌中得到了稳定的、分泌性的表达.

  6. DETECTION OF STRAND BREAKS OF DNA IN HUMAN EARLY CHORIONIC VILLUS CELLS INDUCED BY DIAGNOSTIC ULTRASOUND USING ~(32)P-LABELED ALU HYBRIDIZATION

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Genetic effects of ultrasound on fetus havebeen extensively studied since ultrasonograph waswidely appliedin obstetric practice.In recent years,there have been reports onin vivomolecular geneticeffects of diagnostic ultrasound[1-4].In vitroexperi-ments have found that single-stranded breaks(ssbs)and double-stranded breaks(dsbs)in DNA are themain indices for DNA lesions induced by ultra-sound[5].But,no reports on whether ultrasound cancausein vivossbs and dsbs in DNAare available.Tofurther explore the potent...

  7. Pig endometrium expresses the polyol pathway enzymes necessary to convert glucose to fructose prior to implantation with a shift to chorion expression post-implantation

    Science.gov (United States)

    Glucose and fructose are abundant hexose sugars in pig conceptuses (embryo/fetus and associated placenta). While glucose is mostly catabolized for energy, in vitro studies implicate fructose as a substrate for the biosynthesis of glycoaminoglycans, phospholipids, and nucleic acids as well as a signa...

  8. The impact of Human Chorionic Gonadotropin (hCG) on growth and food conversion efficiency in Royal Danio, Barilius bakeri Day.

    Science.gov (United States)

    Antony, Jomol; Natarajan, P

    2016-03-01

    In aquaculture, growth hormones attract many researchers as they play a key role in the regulation of body weight and metabolism. In this study, we observed the influence of hCG on growth in Barilius bakeri, a common ornamental fish endemic to the Western Ghats, Kerala, India. Juveniles of B. bakeri 2.19 ± 0.92 g were selected and stocked in the experimental tanks of size 90 x 45 x 45 cm. Water quality parameters were examined periodically and were maintained under tolerable limits. The feeds were formulated at 40% protein level. Different levels of hCG i.e. @ 2.5, 5, 7.5, 10, 12.5 and 15 ppm were tested for both short term and long term. Fishes were fed with experimental diets twice daily at the rate of 5% body weight. Fishes were examined fortnightly and their length and weight documented. Their gross body compositions were also analyzed. The results of the study were subjected to ANOVA using statistical package SPSS version 10.1. Feed incorporated with 12.5 ppm hCG showed the best result in providing percentage gain in length, weight and SGR. The FCE, FAE, PER and protein digestibility values were highest in fishes given feed incorporated with 12.5 ppm hCG. PMID:27145636

  9. Analysis of recombinant and native human lutropin/luteotrophin and human chorionic gonadotropin by reversed-phase high performance liquid chromatography

    International Nuclear Information System (INIS)

    Specific RP-HPLC conditions for the analysis of recombinant and native hLH and hCG preparations and of their subunits were set up. Heterodimeric hLH and hCG and their alpha and beta subunits all migrated with significantly different retention times (tR) in the following order of increasing hydrophobicity: alpha-hCG R = 38.35 +- 0.42 min; RSD = 1.1 %; n = 4 preparations), while the hCG main peak ran about 4 % faster when compared to this average value. Human LH, hFSH and hCG peaks could also be identified in the heterogeneous urinary preparations. Quantitative analysis could be validated for the seven homogeneous preparations and accuracy, precision and sensitivity were calculated on the basis of a highly linear dose-response curve (r=0.99998; p<0.0001; n=20). Quantification of the different gonadotropins in the heterogeneous urinary preparations was also carried out, though with clear accuracy limitations. (author)

  10. The Study on Immuno-response and Antisera Properties of Recombinant β-subunit of Human Chorionic Gonadotropin in C57 Black Mouse

    Institute of Scientific and Technical Information of China (English)

    周清平; 申庆祥; 李卫华; 丁训诚

    1997-01-01

    In this study, the immuno-response of recombinant hCG-β and natural hCCwas compctratively investigated by using Freund's adjuvant. The results showed that, the properties and merits of the antibodies elicited by both kinds of hCG-β were similar. The antisera had high affinity for binding with hCG (Kαγβ=5.86×106/mol/L, Kαγβ=8.18×106/mol/L), and were found to be effective in in hibiting the binding of 125I-hCG to receptors in rat testes. Results also indicated that, similar to the antisera induced by natural hCG-β, the recombinant hCG-β induced antisera had capacity of neutralizing the biological activities of hCG. Recombinant hCG-β could be used as an immunogen for contraceptive vaccine.

  11. Human umbilical cord blood mononuclear cells and chorionic plate-derived mesenchymal stem cells promote axon survival in a rat model of optic nerve crush injury

    OpenAIRE

    CHUNG, SOKJOONG; RHO, SEUNGSOO; KIM, GIJIN; Kim, So-Ra; Baek, Kwang-Hyun; KANG, MYUNGSEO; Lew, Helen

    2016-01-01

    The use of mesenchymal stem cells (MSCs) in cell therapy in regenerative medicine has great potential, particularly in the treatment of nerve injury. Umbilical cord blood (UCB) reportedly contains stem cells, which have been widely used as a hematopoietic source and may have therapeutic potential for neurological impairment. Although ongoing research is dedicated to the management of traumatic optic nerve injury using various measures, novel therapeutic strategies based on the complex underly...

  12. The effect of chorionicity and twin-to-twin delivery time interval on short-term outcome of the second twin

    DEFF Research Database (Denmark)

    Hjortø, Sofie; Nickelsen, Carsten; Petersen, Janne;

    2013-01-01

    found no difference in the decrease of pH (p = 0.912) and Apgar (p = 0.609) in relation to increasing time interval. Neonatal unit (NICU) admissions did not differ (p = 0.167). Apgar ≤ 7 (p Apgar...... ≤ 7 and second twin pH ≤ 7.00 (p = 0.003) increased second twin risk of NICU admission. Conclusions: Increasing delivery interval was associated with a significant decrease in pH and Apgar, but there was no difference between MC and DC twins. Low Apgar of the first twin increased the risk of second...

  13. Designing a Long Acting Erythropoietin by Fusing Three Carboxyl-Terminal Peptides of Human Chorionic Gonadotropin β Subunit to the N-Terminal and C-Terminal Coding Sequence

    Directory of Open Access Journals (Sweden)

    Fuad Fares

    2011-01-01

    Full Text Available A new analog of EPO was designed by fusing one and two CTPs to the N-terminal and C-terminal ends of EPO (EPO-(CTP3, respectively. This analog was expressed and secreted efficiently in CHO cells. The in vitro test shows that the activity of EPO-(CTP3 in TFI-1 cell proliferation assay is similar to that of EPO-WT and commercial rHEPO. However, in vivo studies indicated that treatment once a week with EPO-(CTP3 (15 μg/kg dramatically increased (~8 folds haematocrit as it was compared to rHuEPO. Moreover, it was found that EPO-(CTP3 is more effective than rHuEPO and Aranesp in increasing reticulocyte number in mice blood. The detected circulatory half-lives of rHuEPO, Aranesp, and EPO-(CTP3 following IV injection of 20 IU were 4.4, 10.8, and 13.1 h, respectively. These data established the rational for using this chimera as a long-acting EPO analog in clinics. The therapeutic efficacy of EPO-CTP analog needs to be established in higher animals and in human clinical trials.

  14. Levels of the epidermal growth factor-like peptide amphiregulin in follicular fluid reflect the mode of triggering ovulation: a comparison between gonadotrophin-releasing hormone agonist and urinary human chorionic gonadotrophin

    DEFF Research Database (Denmark)

    Humaidan, Peter; Mikkelsen, Anne Lis; Fukuda, Misao;

    2011-01-01

    . INTERVENTION(S): Ovulation triggered with either urinary hCG or GnRH agonist (GnRH-a). Controls: 15 FF samples from small antral follicles (3-9 mm) and 12 FF samples from natural cycle. MAIN OUTCOME MEASURE(S): Follicular fluid concentration of AR, P(4), E(2), vascular endothelial growth factor, and inhibin B...

  15. 1,500 IU human chorionic gonadotropin administered at oocyte retrieval rescues the luteal phase when gonadotropin-releasing hormone agonist is used for ovulation induction: a prospective, randomized, controlled study

    DEFF Research Database (Denmark)

    Al Humaidan, Peter Samir Heskjær; Ejdrup Bredkjaer, Helle; Westergaard, Lars Grabow;

    2010-01-01

    OBJECTIVE: To prospectively assess the reproductive outcome with a small bolus of hCG administered on the day of oocyte retrieval after ovulation induction with a GnRH agonist (GnRHa). DESIGN: Prospective, randomized trial. SETTING: Three hospital-based IVF clinics. PATIENT(S): Three hundred five...

  16. HCG及PLF水平与胎儿发育迟缓的相关研究%Correlation of levels of human chorionic gonadotropin and Placental isoferritin with pathogenesis of Intrauterine fetus growth retardation

    Institute of Scientific and Technical Information of China (English)

    梁一波; 黄思毅; 莫伟; 高卉; 苏小锋

    2008-01-01

    目的 探讨孕妇血浆绒毛膜促性腺激素(HCG)及胎盘异铁蛋白(PLF)水平与胎儿宫内发育迟缓(IUCR)发生、发展的关系.方法 对晚孕早期(孕24~34周)最初正常的1 802例孕妇,根据随访妊娠结局分为IUGR组(64例),用化学发光法进行血浆绒毛膜促性腺激素和胎盘异铁蛋白的水平测定,并与正常组(50例)对比分析.结果 IUGR组β-HCG为(50.63±8.86)ug/L较正常组(18.76±3.69)ug/L明显升高;而PLF(242.76±43.69)mg/L低于正常组(716.95±82.63)mg/L,差异有统计学意义(P<0.01);绒毛膜促性腺激素与胎盘异铁蛋白水平呈负相关(r=-0.563,P<0.01).结论 晚孕早期绒毛膜促性腺激素水平升高和胎盘异铁蛋白水平的降低与胎儿宫内发育迟缓的发生有密切关系.

  17. Association of recombinant bovine somatotropin (rBST with equine chorionic gonadotropin (eCG on antral follicle count and oocyte production in Holstein and Tabapuã heifers

    Directory of Open Access Journals (Sweden)

    Hévea de Morais

    2012-12-01

    Full Text Available The objective of this study was to investigate whether the use of rbST and eCG prior to ultrasound-guided follicular aspiration (OPU improves oocyte yield and quality in Tabapuã and Holstein heifers. The study was conducted in two phases, 20 days apart, in a change-over design. The dominant follicle was ablated two days (D-2 before two treatments: stimulation (6 Holstein and 8 Tabapuã, 500 mg of rbST (Boostin® on D0 and 500 IU of eCG (Folligon on D2; and control (6 Holstein and 8 Tabapuã, in which heifers received injections of the excipient. Heifers were aspirated on D4. Oocytes were subjected to a well established commercial in vitro embryo production protocol (Vitrogen® and embryos were evaluated seven days after fertilization. There was an effect from the interaction of treatment and breed, so that hormonal stimulation increased antral follicle count (2-8 mm in Tabapuã (29.9±2.6 to 41.4±2.6, but not in Holstein heifers (14.4±2.6 to 15.5±2.6. Tabapuã heifers had higher mean antral follicle count than Holsteins (35.6±1.8 vs. 15.0±2.1. The number of viable oocytes was not increased by stimulation in Tabapuã (from 4.7±1.0 to 5.2±1.1 in control and simulation, respectively or in Holstein heifers (1.3±1.9 to 2.0±1.6 in control and simulation, respectively. There was no difference in the percentage of heifers with more than five viable oocytes in the group treated (33 vs 27%. The number of blastocysts was not affected by treatment (1.75 vs. 1.00 in hormonal stimulation and control, respectively. The increase in antral follicle count in the stimulated Tabapuã heifers did not reflect upon oocyte yield. The differential breed response to the hormonal treatment underscores the need for additional tests, especially for the Holstein breed, in order to enhance OPU efficiency.

  18. β-HCG、AFU对原发性肝细胞癌诊断价值%Diagnostic value of β- human chorionic gonadotrophin and fucosidase in the primary hepatic carcinoma

    Institute of Scientific and Technical Information of China (English)

    张云东; 韩兴权; 杨杰

    2006-01-01

    目的 评估人绒毛膜促性腺激素(β-HCG)、α-L-岩藻糖苷酶(AFU)联合检测对原发性肝细胞癌(PHC)的诊断价值.方法 应用微粒子酶免发光技术(MEIA)测定40例PHC,20例肝硬化和20例健康志愿者血清中β-HCG、AFU、甲胎蛋白(AFP)、糖链抗原19-9(CA19-9)、糖链抗原125(CA125)、癌胚抗原(CEA)等6种PHC癌性标志物.结果 PHC患者上述6种指标与肝硬化组和正常人组比较显著增高(P<0.05),Ⅰ期PHC的β-HCG、AFU和AFP与Ⅱ、Ⅲ期PHC相比,变化无显著性.结论 检测β-HCG、AFU有助于发现AFP阴性肝癌,两者联合检测可望提高PHC早期诊断率.

  19. 人绒毛膜促性腺激素(HCG)对杂交黄牛、水牛受胎率的影响%Effects of Human Chorionic Gonadotropin (HCG) on Pregnancy Rate in Hybrid Yellow Cattle and Water Buffalos

    Institute of Scientific and Technical Information of China (English)

    吴师; 李华慧; 覃现才; 宾娟容

    2011-01-01

    近年来,广西象州县的多个牛人工授精品改点的受胎率,黄牛为33%~51%,水牛为35%~55%,母牛的受胎率仍有较大的提升空间.该试验利用HCG能够促进母牛卵泡发育和排卵这一特性,在母牛发情时肌肉注射HCG后进行输精,并观察其对母牛受胎率的影响,以及是否能够提高牛人工授精的效果.试验结果表明,使用HCG能够明显提高牛人工授精的受胎率,同时也提高了工作效率.

  20. 控制性超促排卵中人绒毛膜促性腺激素最佳扳机时间的探讨%Best Time of Human Chorionic Gonadotropin to Trigger Ovulation in Controlled Ovarian Hyperstimulation

    Institute of Scientific and Technical Information of China (English)

    蔡素芬; 张顺吉; 龚斐

    2012-01-01

    本文综合分析了hCG注射日LH、E2、P、E2/P水平,及hCG注射日卵泡大小、每卵泡血清E2水平等各因素与hCG注射时机的相互关系,并指出hCG扳机时机需在卵泡大小达到一定程度,大卵泡达到一定比例,每卵泡E2水平达到200~350 pg/ml之间时,兼顾各激素水平及内膜情况进行综合考虑.%There were two important elements in in vitro fertilization and embryo transplantation (IVF-ET). One is to obtain a synchronous mature egg of high quality which can develop into a plantation potencial embryo after fertilization; the other is a synchronous developped endometrium which allows the embryo implantation. And how to choose the appropriate hCG time to trigger ovulation is crucial to affect above two factors. hCG can stimulate the luteinizing hormone (LH) surge, which has the effect on evoke follicle maturation, ovulation, and corpus luteum funcion. Therefore, hCG injection has become a indispensable part in controlled ovarian hyperstimulation (COH). If the injection of hCG is too earlier, the cumulus cells are too small and attach follicle wall tightly, which will influence the oocyte recovery, and most of collected oocytes may be immatured which will result in low fertilization rate. If hCG injection is too late, endogenous LH surge will occur and make early recovery of oocytes mitosis, so that the oocytes will miss the best time of fertilization, cleavage and implantation after oocyte retrival. Now the studies focus on the best time of hCG injection, and there are no uniform standards in the whole world. Our study reviewed papers of hCG injection and summarize the related factors.

  1. 125I-labelled human chorionic gonadotrophin (hCG) as an elimination marker in the evaluation of hCG decline during chemotherapy in patients with testicular cancer

    DEFF Research Database (Denmark)

    Christensen, T B; Engbaek, F; Marqversen, J;

    1999-01-01

    days (3.6-6.8 days), whereas four out of five patients not achieving long-term remission had half-lives shorter than 3 days. After the third treatment cycle, eight patients who achieved long-term remission had hCG half-lives longer than 3 days (7.4-17.0 days). In these patients, the plasma...... disappearance of [125I]hCG was equivalent to that of hCG. Thus, the slow decline of hCG represented a slow plasma disappearance rather than a hCG production from vital tumour cells and could, consequently, not be used to select patients for additional or intensified chemotherapy. The concept of a fixed half...

  2. Effect of intrauterine insemination after injecting human chorionic gonadotropin at different times on pregnancy rate%注射hCG后不同时间行宫腔内人工受精对妊娠率的影响

    Institute of Scientific and Technical Information of China (English)

    江梅; 靳镭; 徐婕; 聂睿; 彭芳昕

    2010-01-01

    目的:探讨注射hCG后不同时间行宫腔内人工受精(Intrauterine insemination,IUI)对妊娠率的影响.方法:回顾性分析同济医院生殖中心472对夫妇实施IUI治疗的561个周期.根据注射hCG后行IUI的不同时间分为2组:hCG后24 hIUI组和36 h IUI组,比较两组IUI后的排卵时间及其对妊娠率的影响.结果:hCG后24 h IUI 359个周期和hCG后36 h IUI 202个周期的妊娠率分别为10.86%、10.89%,P>0.05,差异无统计学意义;两组24 h内排卵的344个周期妊娠率为13.08%,明显高于24 h内未排卵的217个周期妊娠串7.37%,P<0.05,差异有统计学意义.结论:影响IUI结局的因素并非注射hCG后IUI的时间,而是IUI后的排卵时间,距排卵时间越近妊娠率越高.阴道B超联合尿LH半定量试纸检测可以较准确预测排卵时间,有利于提高其周期妊娠率.

  3. 血清β-绒毛膜促性腺激素和孕酮检测在先兆流产早期诊断中的临床意义%Clinical significance of serum β-human chorionic gonadotropin and progesterone detections in early diagnosis of patientswith threatened miscarriage

    Institute of Scientific and Technical Information of China (English)

    朱学菁

    2015-01-01

    目的 探讨血清β-绒毛膜促性腺激素(β-hCG)和孕酮检测在先兆流产早期诊断中的临床意义.方法 240例早孕孕妇分为正常妊娠组(n=76)、先兆性流产组(n=87)和流产组(n=77).比较3组孕妇血清孕酮和β-hCG水平.结果 妊娠第4、5、6周,流产组孕妇的血清孕酮水平显著低于其他2组(P<0.05),先兆性流产组孕妇的血清孕酮水平显著低于正常妊娠组(P<0.05);妊娠第4周时,3组孕妇血清βhCG水平无显著差异(P>0.05);妊娠第5、6周,3组孕妇血清β-hCG水平均显著上升(P<0.05),且流产组孕妇的血清βhCG水平显著低于先兆流产组(P<0.05),先兆流产组的血清β-hCG水平显著低于正常妊娠组(P<0.05).结论 妊娠早期孕妇的血清β-hCG水平和孕酮水平可作为辅助诊断先兆妊娠和预测妊娠结局的指标.

  4. Sincronização do estro, indução da ovulação e fertilidade de ovelhas deslanadas após tratamento hormonal com gonadotrofina coriônica eqüina Estrus synchronization, ovulation induction and fertility in hairless ewes treated with equine chorionic gonadotrophin

    Directory of Open Access Journals (Sweden)

    F.E.F. Dias

    2001-10-01

    Full Text Available Utilizou-se a sincronização do estro com esponjas vaginais de 30mg de acetato de fluorogestona durante 12 dias para avaliar o efeito de diferentes doses de eCG sobre o desempenho reprodutivo de ovelhas. Na retirada das esponjas as ovelhas foram divididas em três grupos para receberem 0 (n=26, 200 (n=30 ou 400UI (n=30 de eCG. O estro foi detectado a cada 12h utilizando-se um rufião. As fêmeas foram inseminadas por laparoscopia 60h após a retirada das esponjas. Realizaram-se colheitas de sangue aos 5 e 18 dias pós-inseminação para dosagem de progesterona e determinação do número de ovulações e prenhezes, respectivamente. A fertilidade foi verificada por ecografia aos 60 dias e ao parto. Das 86 ovelhas 70,9% apresentaram estro. Essa porcentagem foi maior (PThe aim of this experiment was to evaluate the effect of different doses of eCG on reproductive performance of ewes. The estrus was synchronized with vaginal sponges with 30mg FGA during 12 days. At the time of sponge removal, ewes were distributed in three groups: 0 (n=26, 200IU eCG (n=30 and 400IU eCG (n=30. The estrus was detected by aid of a vasectomized ram. The ewes were inseminated by laparoscopy, 60h after sponge removal. Blood samples were collected at 5 and 18 days after insemination, in order to determine progesterone concentration. It was observed 70.9% of ewes in estrus after the end of treatment. The females that received eCG showed higher (P<0.05 percentage of estrus: 96.7% (400IU and 76.7% (200IU versus 34.6% (0 IU. The interval between the end of treatment and estrus onset was higher (P<0.05 in the group that did not receive eCG (54.7±6.3h in comparison to the groups that received 200IU (45.9±7.8 h and 400 IU eCG (40.4±10.3h. A lower (P<0.05 number of ovulating and pregnant ewes in the group that did not receive eCG was observed. The eCG withdraw resulted in negative effect on reproductive performance of ewes.

  5. Vilosidades e outras estruturas coriônicas encontradas no sangue periférico de gestantes: número, tamanho e uma técnica para sua recuperação = Villus and other chorionic structures found at the peripheral blood of pregnant women: number, size, and a technique for their recovery

    Directory of Open Access Journals (Sweden)

    Luz, Nilo Pereira

    2008-01-01

    Conclusões: a fixação imediata das amostras foi apresentada como um novo método para recuperar as estruturas coriônicas presentes no sangue periférico de gestantes. Elas foram identificadas, contadas e medidas. Sua quantidade e dimensões foram grandes. Os mecanismos que podem explicar seu surgimento e seu destino, bem como o significado de sua presença e quantidade no sangue periférico das gestantes foram discutidos.

  6. Detection of chromosome aneuploidy and copy number variation in abortion fetus or chorionic villi using NGS technology%NGS技术检测自然流产胚胎或绒毛染色体非整倍体及拷贝数变异的研究

    Institute of Scientific and Technical Information of China (English)

    赵佳; 毕川; 高雅; 季刚; 汪凌云; 张红云; 李云; 王军; 王威

    2015-01-01

    目的 探索新一代测序技术(NGS)在检测自然流产胚胎或绒毛组织染色体非整倍体和拷贝数变异(CNV)应用中的价值.方法 选择20例自然流产患者的绒毛进行染色体核型分析,同时应用NGS技术进行染色体非整倍体和拷贝数变异的检测,并以染色体核型分析结果为“金标准”进行NGS方法的评估.后于201 3年共收集1 074例自然流产胚胎或绒毛组织,应用NGS技术完成染色体非整倍体和CNV的检测,并对检测结果进行分析.结果 20例自然流产样本的NGS结果与核型分析结果对比,检测灵敏度和特异性均为100%.临床检测的1 074例流产组织样本中,42例样本DNA不符合质控标准,实际完成检测1032例.1 032例组织样本中阳性445例(43.12%),其中非整倍体369例(82.92%),以16、X、22、21、15、1 8号染色体高发;CNV共76例(17.08%),阳性样本集中发生在8-12w.阴性587例(56.88%).根据孕妇年龄将样本分为三组比较,差异有统计学意义(P<0.01).结论 NGS技术用于检测流产组织的非整倍体和拷贝数变异具有较高的灵敏度和特异性,是适用于临床的有效检测方法.

  7. 激光捕获显微切割技术诊断石蜡切片中早期胚胎成分与亲权关系的研究%Separation of chorionic villi from paraffin-embedded early abortion materials and confirmation of parent-child relationship by laser capture microdissection

    Institute of Scientific and Technical Information of China (English)

    李艮平; 李彩霞; 郝建文; 万立华; 胡兰; 韩俊平; 杨帆

    2012-01-01

    目的 通过激光捕获显微切割技术捕获石蜡切片中胚胎绒毛细胞,进行低体积扩增,实现胚胎个体分型,证实亲子关系.方法 通过激光捕获显微技术捕获石蜡切片上胚胎绒毛细胞,设置20、30、40个细胞数目组,分别捕获不同数目绒毛细胞,利用低体积扩增仪进行1.5μl体系PCR扩增,采用3130遗传分析仪得出胚胎个体分型图谱,并比较各细胞数目组胚胎个体识别的检出率、等位基因丢失率和非特异性扩增情况.最后进行实际案例应用.结果 捕获20个胚胎绒毛细胞即可获得胚胎个体分型,40个细胞数目组的胚胎分型检出率最高,等位基因丢失率和非特异性扩增率最低.20个细胞数目反之.结论 激光捕获显微切割技术结合低体积扩增技术可应用于石蜡切片,实现胚胎与母体成分的精确分离并确证亲权关系.%Objective It remains difficult to genotype the mixture of maternal and embryo from early abortion tissues to investigate forensic evidence and confirm parent-child relationship. Our study was aimed to confirm the parent-child relationship by using laser capture microdissection ( LCM) to capture the embryonic villi cells in paraffin-embedded section for genotyping embryonic individual via ultralow volume PCR amplification. Methods Embryonic villi cells in the paraffin sections was captured by LCM technology. With the setting of cell numbers at 20, 30 and 40 cells as a group, villus cells of different cell numbers were captured and subjected to PCR amplification at low volume of 1. 5 μl by using 3130 Genetic Analysis instrument to yield embryonic genotyping maps. The artificial alleles, detection rate and allelic dropout rate of each group were compared. The technology was also applied on an actual case. Results Embryonic villi cells in the paraffin sections were captured by LCM technology. With the setting of cell numbers at 20, 30 and 40 cells for group, villus cells of different cell numbers were captured and subjected to PCR amplification at low volume of 1. 5 jμ1, and embryonic genotyping maps were obtained. The rate of embryo genotyping in the group of 40 cells was the highest whereas the rate of allele loss and non-specific amplification was the lowest. Vise versa for the group of 20 cells. Conclusion LCM combined with the low volume amplification technique can be applied to paraffin-embedded tissue sections to achieve accurate separation of the embryo and maternal components and obtain conclusive evidence for parental rights.

  8. Efecto de diferentes dosis de gonadotrofina coriónica equina sobre la respuesta reproductiva de hembras ovinas con un tratamiento para inducción de celos Effect of different doses of equine chorionic gonadotropin on reproductive performance in sheep with oestrus induction treatment cycle

    Directory of Open Access Journals (Sweden)

    R Catalano

    2007-12-01

    Full Text Available El objetivo de este trabajo fue comparar el efecto de diferentes dosis de eCG en un tratamiento para inducción de celos en borregas (Ensayo 1 y ovejas (Ensayo 2 (Frisona x Corriedale en anestro estacional sobre variables reproductivas. Se utilizaron esponjas intravaginales con 60 mg de acetato de medroxiprogesterona por 10 días y al retiro de las mismas se administraron 300 UI (grupo G300 ó 500 UI (grupo G500 de eCG. El porcentaje de celo no fue diferente entre grupos (Ensayo 1 = 100%; Ensayo 2 = 81,2%. En el Ensayo 1 se observó una tendencia a diferir en el porcentaje de borregas que ovularon (G300 = 50,0%; G500= 90,0%. P=0,06 y una diferencia significativa en el porcentaje de preñez (G300 = 20,0%; G500 = 70,0%. PThe aim of this study was to evaluate the reproductive performance of seasonally anoestrus hoggets (Trial 1 and ewes (Trial 2 (Frisona x Corriedale treated with intravaginal sponges containing 60 mg of medroxiprogesterone acetate during 10 days and different doses of eCG. At the end of treatment, 300 IU of eCG (group G300 or 500 IU of eCG (group G500 were injected intramuscularly. Oestrus rate was not different between groups (Trial 1= 100%; Trial 2= 81.2%; P>0.05. In the trial 1, the percentage of hoggets that ovulated tended to differ between groups (G300= 50.0%; G500= 90.0%; P=0.06 and the pregnancy rate was signifi cant different (G300= 20.0%; G500= 70.0%; P<0.05. In the trial 2, the pregnancy and the fertility rates were signifi cant different between groups G300 and G500 (6.3 and 9.1 vs 56.3 and 60.0% respectively; P<0.05. It was concluded that the use of 300 UI of eCG in a treatment of oestrus induction of seasonally anoestrus hoggets and ewes affect negatively the reproductive performance compared with the use of 500 UI of eCG.

  9. Analysis of recombinant and native human lutropin/luteotrophin and human chorionic gonadotropin by reversed-phase high performance liquid chromatography; Analise de luteotrofina humana e de gonadotrofina corionica humana, recombinante e natural, por cromatografia liquida de alta eficiencia em fase reversa

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, Beatriz Elane de

    2009-07-01

    Specific RP-HPLC conditions for the analysis of recombinant and native hLH and hCG preparations and of their subunits were set up. Heterodimeric hLH and hCG and their alpha and beta subunits all migrated with significantly different retention times (t{sub R}) in the following order of increasing hydrophobicity: alpha-hCG < alpha-hLH < hCG < hLH < -beta-hCG < beta-hLH. With basis on these conditions, a total of eleven preparations were studied: the International Standard of recombinant hLH-WHO 96/602, a commercial recombinant and two highly purified pituitary hLH, a recombinant and two urinary hCG preparations and four heterogeneous urinary products containing hLH + hFSH. All hLH preparations showed very similar retention times for the main peak (t{sub R} = 38.35 +- 0.42 min; RSD = 1.1 %; n = 4 preparations), while the hCG main peak ran about 4 % faster when compared to this average value. Human LH, hFSH and hCG peaks could also be identified in the heterogeneous urinary preparations. Quantitative analysis could be validated for the seven homogeneous preparations and accuracy, precision and sensitivity were calculated on the basis of a highly linear dose-response curve (r=0.99998; p<0.0001; n=20). Quantification of the different gonadotropins in the heterogeneous urinary preparations was also carried out, though with clear accuracy limitations. (author)

  10. Efecto de diferentes dosis de gonadotrofina coriónica equina sobre la respuesta reproductiva de hembras ovinas con un tratamiento para inducción de celos Effect of different doses of equine chorionic gonadotropin on reproductive performance in sheep with oestrus induction treatment cycle

    OpenAIRE

    Catalano, R.; Teruel, M.; J Cabodevila; S Callejas

    2007-01-01

    El objetivo de este trabajo fue comparar el efecto de diferentes dosis de eCG en un tratamiento para inducción de celos en borregas (Ensayo 1) y ovejas (Ensayo 2) (Frisona x Corriedale) en anestro estacional sobre variables reproductivas. Se utilizaron esponjas intravaginales con 60 mg de acetato de medroxiprogesterona por 10 días y al retiro de las mismas se administraron 300 UI (grupo G300) ó 500 UI (grupo G500) de eCG. El porcentaje de celo no fue diferente entre grupos (Ensayo 1 = 100%; E...

  11. Gonadotrofina coriônica eqüina: purificação, caracterização e resposta ovariana em ovinos e suínos Equine chorionic gonadotrophin: purification, characterization and ovarian activity in ewes and gilts

    OpenAIRE

    José Antonio Guimarães Aleixo; João Carlos Deschamps; Vilceu Bordignon; Cláudio Alves Pimentel; José Carlos Ferrugem Moraes

    1995-01-01

    A gonadotrofina coriônica equina (eCG) foi purificada e caracterizada com respeito ao grau de pureza e atividade biológica. A pureza de quatro preparações foi determinada por eletroforese e a atividade biológica pelo incremento do peso ovariano de ratas imaturas (40 - 50g) e pela indução de ovulação em ovelhas e leitoas. A análise eletroforética revelou a presença de três bandas polipeptídicas. A atividade biológica media foi de 313 UI/mg de proteína. Sessenta e cinco (65) ovelhas, fora da es...

  12. The effect of epidermal growth factor and fibroblast growth factor on the proliferation of human chorionic cells in vitro%表皮生长因子和成纤维细胞生长因子对绒毛细胞体外增殖的影响

    Institute of Scientific and Technical Information of China (English)

    唐雪莲; 赵坤; 谢梅青; 谢建生; 姚吉龙; 古艳

    2013-01-01

    Objective; To study the effect of epidermal growth factor (EGF) and fibroblast growth factor (FGF) on the proliferation of human trophoblastic cells. Methods: 12 samples of human first trimester villus tissue and 16 samples villus tissue of reappear a-bortion were used. The confluent cells were digested, cultured with different concentrations of EGF, FGF, EGF + FGF or for 24, 48 and 72h. The proliferation and apoptosis of cells were detected by MTT method and TUNEL staining. Results: The speed of cell growth was increased with the concentration of 10. Ong/ml EGF and FGF ( P 0. 05 ). Conclusion; EGF or FGF can control the proliferation of human trophoblastic cells. EGF or FGF significantly promoted the proliferation of human trophoblastic cells at a definite concentration. The cooperation of EGF and FGF exhibits the best result.%目的 探讨表皮生长因子(EGF),成纤维细胞生长因子(FGF),对人绒毛滋养层细胞体外生长的调控作用.方法 对12例人早孕绒毛离体组织及16例复发性流产绒毛进行体外分离培养,细胞汇合后消化,做细胞增殖试验,在相同的生长期加入EGF,FGF,EGF+FGF培养24,48,72h,用MTT法、DNA切口末端标记法测定EGF,FCF对2组绒毛滋养层细胞的作用.结果 10.0ng/ml EGF及FGF刺激细胞生长,EGF +FGF刺激细胞生长的作用最强,10.0ng/ml EGF,FCF不刺激细胞凋亡,两组结果具有一致性,MTT法、DNA切口末端标记法结果一致.结论 人滋养层细胞的生长受到EGF,FGF的调控,EGF及FGF具有促进细胞增殖作用;EGF,FGF联合应用具有最佳的协同效应.

  13. Efecto de la administración de diferentes dosis de gonadotrofina coriónica equina sobre la respuesta ovárica y el desarrollo in vitro de embriones de ratón Effect of different doses of equine chorionic gonadotropin on ovary response and in vitro mouse embryo development

    Directory of Open Access Journals (Sweden)

    M. T. Teruel

    2006-12-01

    Full Text Available El objetivo del trabajo fue evaluar el efecto de diferentes dosis de gonadotrofina coriónica equina (eCG (5, 7,5 o 10 UI sobre parámetros ováricos y desarrollo in vitro de embriones de hembras ratón Balb C. El peso y diámetro ovárico fueron superiores en animales tratados con 7,5 y 10 UI de eCG que en animales controles (PThe aim of this study was to evaluate the effect of different doses of equine gonatotropin hormone (eCG (5, 7.5 or 10 IU on the ovary response and in vitro embryo development in Balb C mice. The ovary weight and diameter increased for 7.5 and 10 IU of eCG compared to control group (P0.05. In vitro, the differentiation was not modified by the dose level. The hatching rate at 96 h was higher for embryos from 7.5 IU compared to 10 IU (82.22 vs 64.39, P<0.05. We concluded that, the percentage of normal oocytes and morulae and the differentiation rate are not dependent of the dose of eCG, however, the hatching in vitro is dose eCG dependent.

  14. Desenvolvimento folicular no pós-parto de vacas da raça Gir tratadas com acetato de buserelina (GnRH ou gonadotrofina coriônica humana (hCG Follicular development in Gir breed cows (Bos indicus treated with buserelin acetate or human chorionic gonadotrophin during the postpartum period

    Directory of Open Access Journals (Sweden)

    Álan Maia Borges

    2004-12-01

    Full Text Available Foram utilizadas 22 vacas da raça Gir (Bos indicus em anestro pós-parto, amamentando e mantidas sob regime de pastejo. O padrão de crescimento folicular foi estudado em 15 animais, enquanto os outros sete foram utilizados somente no acompanhamento da resposta aos tratamentos hormonais. Ultra-sonografia em tempo real foi realizada diariamente, do quarto ao qüinquagésimo dia de lactação, por meio de aparelho equipado com probe de 5MHz. Nenhum animal apresentou estro, ovulação ou luteinização folicular durante o período de avaliação. Verificaram-se quatro a oito ondas foliculares surgindo em intervalos de seis a sete dias, cujo diâmetro máximo dos folículos dominantes e subordinados foi de 8,9±0,6 e 6,3±0,7mm, respectivamente. Aos 56 dias pós-parto, as 22 vacas foram divididas em dois grupos: 11 tratadas com 4,2 mig de acetato de buserelina e 11 com 3.000UI de hCG. A ovulação, regressão e luteinização folicular foram verificadas em animais dos dois tratamentos e uma nova onda folicular surgiu dentro de dois a três dias após a terapia hormonal. Corpos lúteos de menor tamanho e concentrações de progesterona abaixo de 1ng/mL indicaram baixa atividade luteal.This experiment was carried out with 22 anestrus Gir cows (Bos indicus, with the postpartum follicular growth studied in 15 of these suckling and kept on pasture, while the seven others were used only for hormonal treatments. Daily real time ultrasonography was done from 4th to the 50th day, using B-mode ultrasound scanner equipped with a linear array 5.0MHz probe. No animal showed estrus, ovulation or follicular luteinization during de experimental period. Four to eight follicular waves emerged each six or seven days and maximum diameter of dominant and subordinate follicles were 8.9±0.6mm and 6.3±0.7mm, respectively. On day 56 of postpartum all cows were separated in two groups: 11 cows were treated either with 4.2 mug of buserelin acetate and 11 with 3,000UI of hCG. Ovulation, follicular regression and luteinization were observed in the two treatments animals, and one new follicular wave emerged within two to three days after the hormonal treatments. Small size corpus luteum and progesterone concentrations under 1ng/mL showed lower luteal activity.

  15. MOSAICISM CONFINED TO PLACENTA IN PREGNANCIES WITH ADVERSE OUTCOME

    Institute of Scientific and Technical Information of China (English)

    向阳; KarinSundberg; BjarneBeck; 孙念怙

    1995-01-01

    Chorionic villi and feral tissues from 50 pathological human conceptions ar gesrarional weeks 9-40 were cultured and cytogenetically analyzed to explore the existence of chromosomal mosaicism confined to the extraembryonic tissues and to clarify the relationship between confined placental mosaicism and adverse outcome of pregnancy. Chorionic villi and fetal rlssues from 12 second trimester gesrations terminated for social reasons served as a control group. In two pathological gestations, true mosaicism was found exclusively in chorionic cells and could not be confirmed in cells derived from the fetal tissues, One of these was severely growth retarded, Concordant results were obtained in all other cases,

  16. Genetics Home Reference: familial male-limited precocious puberty

    Science.gov (United States)

    ... and luteinizing hormone triggers these cells to produce androgens. Androgens, including testosterone, are the hormones that control male ... chorionic gonadotropin. In males, the overactive receptor causes excess production of testosterone, which triggers male sexual development ...

  17. How Is Testicular Cancer Diagnosed?

    Science.gov (United States)

    ... proteins called tumor markers , such as alpha-fetoprotein (AFP) and human chorionic gonadotropin (HCG). When these tumor ... that there is a testicular tumor. Rises in AFP or HCG can also help doctors tell which ...

  18. General Information about Testicular Cancer

    Science.gov (United States)

    ... are used to detect testicular cancer: Alpha-fetoprotein (AFP). Beta-human chorionic gonadotropin (β-hCG). Tumor marker ... places in the body, and blood levels of AFP, β-hCG, and LDH). Type of cancer. Size ...

  19. Experiment list: SRX100887 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ranes were collected from women who underwent planned cesarean delivery at term, before labor and without ru...an || cell description=chorion cells (outermost of two fetal membranes), fetal membranes were collected from women

  20. Prenatal diagnostic procedures used in pregnancies with congenital malformations in 14 regions of Europe

    NARCIS (Netherlands)

    Garne, E; Loane, M; de Vigan, C; Scarano, G; de Walle, H; Gillerot, Y; Stoll, C; Addor, MC; Stone, D; Gener, B; Feijoo, M; Mosquera-Tenreiro, C; Gatt, M; Queisser-Luft, A; Baena, N; Dolk, H

    2004-01-01

    Objective To investigate outcomes of ultrasound investigations (US) and invasive diagnostic procedures in cases of congenital malformations (CM), and to compare the use of invasive prenatal test techniques (amniocentesis (AC) versus chorionic villus sampling (CVS)) among European populations. Design

  1. What Is Testicular Cancer?

    Science.gov (United States)

    ... increases blood levels of HCG (human chorionic gonadotropin). Teratoma: Teratomas are germ cell tumors with areas that, ... There are 3 main types of teratomas: Mature teratomas are tumors formed by cells similar to cells ...

  2. Drug: D03478 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available gonadotropin alfa D03478 Choriogonadotropin alfa (USAN/INN) USP drug classification [BR:br08302] Hormonal Agents, Stimulant/Replaceme...nt/Modifying (Pituitary) Chorionic Gonadotropin D03478 C

  3. A modest but significant effect of CGB5 gene promoter polymorphisms in modulating the risk of recurrent miscarriage

    DEFF Research Database (Denmark)

    Rull, Kristiina; Christiansen, Ole Bjarne; Nagirnaja, Liina;

    2013-01-01

    To confirm the effect of single nucleotide polymorphisms (SNPs) in chorionic gonadotropin beta (CGB) genes in modulating the susceptibility to recurrent miscarriage (RM) in Danes and in a meta-analysis across Danes and the discovery samples from Estonia and Finland.......To confirm the effect of single nucleotide polymorphisms (SNPs) in chorionic gonadotropin beta (CGB) genes in modulating the susceptibility to recurrent miscarriage (RM) in Danes and in a meta-analysis across Danes and the discovery samples from Estonia and Finland....

  4. Progestin Exposure Before Gonadotropin Stimulation Improves Embryo Development after In Vitro Fertilization in the Domestic Cat1

    OpenAIRE

    Pelican, Katharine M.; Spindler, Rebecca E.; Pukazhenthi, Budhan S.; Wildt, David E.; Ottinger, Mary A.; Howard, JoGayle

    2010-01-01

    This study investigated the influence of progestin priming and ovarian quiescence on response to exogenous gonadotropin stimulation in the cat. Because a subpopulation of cats routinely ovulated spontaneously, there also was the opportunity to examine the ovary's reaction to the added impact of endogenously secreted progestagen. Queens were given 1) equine chorionic gonadotropin (eCG) plus human chorionic gonadotropin (hCG) only (control; n = 9 cats), 2) GnRH antagonist (antide) injections fo...

  5. Oral Progestin Priming Increases Ovarian Sensitivity to Gonadotropin Stimulation and Improves Luteal Function in the Cat1

    OpenAIRE

    Stewart, Rosemary A.; Pelican, Katharine M.; Crosier, Adrienne E.; Pukazhenthi, Budhan S.; Wildt, David E.; Ottinger, Mary Ann; Howard, JoGayle

    2012-01-01

    As the only domesticated species known to exhibit both induced and spontaneous ovulation, the cat is a model for understanding the nuances of ovarian control. To explore ovarian sensitivity to exogenous gonadotropins and the influence of progestin priming, we conducted a study of queens that were down-regulated with oral progestin or allowed to cycle normally, followed by low or high doses of equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG). Our metrics included 1) f...

  6. Reproductive system

    Institute of Scientific and Technical Information of China (English)

    1995-01-01

    950267 Secretion of sex hormone in response to sin-gle or repeated administration of human chorionic go-nadotropin in adult males.LI Jianyuan(李江源),etal.General Hosp PLA.Beijing,100853.Med J ChinPLA 1994:19(6):422-424.Fourteen healthy adult males accepted intramuscular2000 IU of human chorionic gonadotropin (HCG) in 3ways such as single injection,one injection daily for 2successive days and 12 rejections in

  7. Effective treatment for malignant mediastinal teratoma.

    OpenAIRE

    Parker, D; Holford, C P; Begent, R. H.; Newlands, E S; Rustin, G J; Makey, A R; Bagshawe, K D

    1983-01-01

    Primary malignant mediastinal teratoma is a rare tumour previously regarded as inevitably fatal. In a series of eight male patients with a mean age of 24 years five remain alive and well. All patients showed raised serum concentrations of human chorionic gonadotrophin or alpha fetoprotein. The patients were treated with intermittent combination chemotherapy that included cisplatin. Six patients responded to chemotherapy with a fall in human chorionic gonadotrophin or alpha fetoprotein to near...

  8. A meta-analysis of screening Down's syndrome with maternal pregnancy associated plasma protein-A combined with free beta human chorionic gonadotropin and fetal nuchal translucency in the first trimester%妊娠相关血浆蛋白A联合游离人绒毛膜促性腺激素、胎儿颈项透明层筛查唐氏综合征的meta分析

    Institute of Scientific and Technical Information of China (English)

    刘举珍; 卢颖州; 钱敏; 杨兴华; 何燕; 何电

    2014-01-01

    目的:系统评价妊娠相关血浆蛋白-A(PAPP-A)联合游离人绒毛膜促性腺激素(F-βHCG)、胎儿颈项透明层(NT)在孕早期筛查唐氏综合征的价值.方法:按照系统评价的方法,计算机检索Medline (PubMed)、EMBase、Cochrane library、万方数据库、中国知网(CNKI)和中国生物医学文献光盘数据库(CBM)发表的中英文文献,根据纳入和排除标准选择合格的文献进行方法学质量评价,并摘录数据;采用Meta Disc 1.4软件对数据进行异质性检验和meta分析,计算合并效应量.结果:符合条件的文献共12篇,累计唐氏综合征患儿783例,正常婴儿213 943例.这12项研究不存在阈值效应异质性和非阈值效应异质性,汇总的灵敏度为88%(95%CI:0.86~ 0.90),特异度为95%(95%CI:0.95~ 0.95),AUC为0.97,阳性似然比为19.17(95%CI:16.8~ 21.9).结论:当假阳性为5%时,该筛查方法的灵敏度为88%,在唐氏综合征早期筛查中具有较高的应用价值.

  9. Uso da gonadotrofina coriônica eqüina em receptoras de embriões para avaliar o incremento da progesterona endógena no dia da inovulação e sua correlação com a taxa de prenhez Use of equine chorionic gonadotrophin in heifers embryo receptors to evaluate the increment of endogenous progesterone in the inovulation day in relation to pregnancy rate

    OpenAIRE

    Carlos Martins Rodrigues; Elias Nunes Martins; Alencariano José da Silva Falcão; Gentil Vanini de Moraes; Egon José Fuck; Rejane Machado Cardozo; Ciro Moraes Barros

    2002-01-01

    O experimento foi realizado com a finalidade de avaliar a influência da administração de gonadotrofina coriônica eqüina (eCG) em receptoras de embrião na formação de corpos lúteos acessórios e a correlação com o índice de prenhez. Utilizaram-se 64 novilhas de 18 a 30 meses de idade, mestiças Simental, com peso médio de 400 kg, as quais foram avaliadas por palpação retal, entre os dias 7 e 12 após a manifestação do cio, considerado como cio base. Neste intervalo de 7 a 12 dias após o cio, defi...

  10. Uso da gonadotrofina coriônica eqüina em receptoras de embriões para avaliar o incremento da progesterona endógena no dia da inovulação e sua correlação com a taxa de prenhez Use of equine chorionic gonadotrophin in heifers embryo receptors to evaluate the increment of endogenous progesterone in the inovulation day in relation to pregnancy rate

    Directory of Open Access Journals (Sweden)

    Carlos Martins Rodrigues

    2002-04-01

    Full Text Available O experimento foi realizado com a finalidade de avaliar a influência da administração de gonadotrofina coriônica eqüina (eCG em receptoras de embrião na formação de corpos lúteos acessórios e a correlação com o índice de prenhez. Utilizaram-se 64 novilhas de 18 a 30 meses de idade, mestiças Simental, com peso médio de 400 kg, as quais foram avaliadas por palpação retal, entre os dias 7 e 12 após a manifestação do cio, considerado como cio base. Neste intervalo de 7 a 12 dias após o cio, definido como dia zero (D0, os animais que se encontravam com corpo lúteo fisiológico foram divididos, aleatoriamente, em 4 tratamentos, sendo que os animais do tratamento 1 (controle, n=21, receberam solução fisiológica, via intramuscular (IM; no tratamento 2 (n=14 receberam 200 UI de eCG (FOLLIGON® - Intervet, via IM; no tratamento 3 (n=16, receberam 400 UI de eCG, via IM; e no tratamento 4 (n=13, receberam 600 UI de eCG, via IM. Transcorridos 2 dias (D2 da aplicação do eCG, administrou-se 2 mL de prostaglandina F2 alfa (PROSOLVIN® - Intervet por animal. Em seguida, observou-se a manifestação de cio e, 7 dias após a sua detecção, avaliou-se os animais, por ultrassonografia, para seleção das fêmeas aptas a receber os embriões congelados. Vinte e três dias após a inovulação, procedeu-se ao diagnóstico de prenhez por ultrassonografia. Não houve diferença (P > 0,05 para a taxa de prenhez. Concluiu-se que o uso de eCG em receptoras de embrião congelado não melhorou os índices de aproveitamento das receptoras nem a taxa de prenhez.The experiment was carried out to evaluate the influence of pregnant Mare Serum Gonadotrophin (eCG administration in embryo receptors on the formation of accessory corpus luteum and correlation with the pregnancy rate. Sixty-four beef cattle heifers -18 to 30 months old were used, ½ Simental, with 400 kg of average live weight. The heifers were evaluated through rectal palpation between days 7 to 12 after heat detection, considered as heat base. In this interval from 7 to 12 days after the heat, defined as zero day (D0, the animals identified with physiological corpus luteum were divided in four treatments. The animals in treatment 1 (control, n=21 received physiologic solution intramuscle (IM; in treatment 2 (n=16 received 200 IU of eCG (FOLLIGON - Intervet, IM; in treatment 3 (n=14 received 400 IU of eCG IM; and in treatment 4 (n=13 received 600 IU of eCG IM. Two days after (D2 eCG application all the animals received 2.0 mL of prostaglandin F2 alpha (PROSOLVIN - Intervet. Following, the animals' heat manifestation was observed, and, seven days after heat detection, the animals were evaluated for ultrasonography to select females able to receive frozen embryos. Twenty-three days after inovulation, the pregnancy diagnostic was detected through ultrasonography. There was no statistic difference (p > 0.05 for pregnancy rate. These results show that eCG used in receptors of frozen embryos neither improved the receptors' performance rate, nor the pregnancy rate.

  11. Gonadotrofina coriônica humana e hormônio liberador de gonadotrofina como indutores da reprodução do jundiá - DOI: 10.4025/actascianimsci.v30i3.929 Human chorionic gonadotropin and gonadotropin-releasing hormone as breeding inducers of jundiá - DOI: 10.4025/actascianimsci.v30i3.929

    Directory of Open Access Journals (Sweden)

    Paulo César Falanghe Carneiro

    2008-11-01

    Full Text Available Este trabalho foi realizado com o objetivo de avaliar parâmetros reprodutivos de machos e fêmeas de jundiá adultos após a aplicação do HCG (gonadotrofina coriônica humana e do GnRHa (hormônio liberador do hormônio gonadotrópico e compará-los àqueles obtidos quando usado o extrato hipofisário da carpa comum. Etapa 1 (Machos - quatro grupos, com oito machos cada, receberam os seguintes tratamentos: M1: sem hormônio; M2: extrato hipofisário 0,5 mg kg-1; M3: HCG 200 UI kg-1; M4: GnRHa+inibidor dopaminergético 0,1 glóbulo(Ovopel® kg-1. Etapa 2 (fêmeas – 36 fêmeas foram divididas em cinco grupos: F1: extrato hipofisário em duas aplicações, 0,5 e 5,0 mg kg-1; F2: HCG em duas aplicações, 200 e 400 UI kg-1; F3: HCG 400 UI kg-1 em dose única; F4: HCG 1000 UI kg-1 em dose única; F5: GnRHa+inibidor dopaminergético 1 glóbulo(Ovopel® kg-1. O uso do extrato hipofisário da carpa comum aumentou significativamente o volume de sêmen liberado e estimulou maior quantidade de fêmeas à liberar óvulos. O HCG e o GnRHa não apresentaram resultados positivos no tocante à reprodução induzida do jundiá, nas doses utilizadas neste estudo.Hormone acquisition represents high cost, justifying the importance of studies searching for better results on inducing fish reproduction in laboratory conditions. This study analyzed the efficiency of HCG and GnRHa for gonad maturation and gamete production in jundiá, and compare the results to those using pituitary extract. Stage 1 (Males - Four groups with eight males each received the following treatments: M1: Without hormone; M2: Pituitary extract 0.5 mg/kg; M3: HCG 200 UI/kg; M4: GnRHa+dopamine receptor antagonist 0.1 pellet/kg. Stage 2 (Females – Thirty six females were separated in 5 groups: F1: Pituitary extract in two applications, 0.5 and 5.0 mg/kg; F2: HCG in two applications, 200 and 400 UI/kg; F3: HCG 400 UI/kg in a single dose; F4: HCG 1000 UI/kg in a single dose; F5: GnRHa+dopamine receptor antagonist 1 pellet/kg. The pituitary extract increased significantly the volume of semen and was more efficient in the final gonad maturation and ovulation, stimulating a higher number of females. The pituitary extract is efficient for the induction of jundia males and females during reproduction procedures, corroborating preview information. HCG does not induce jundiá breeders with doses employed in this study and GnRHa is not suitible to induce jundia breeders under dose recommended by the manufacturer to other tropical species.

  12. Release of LHRH-activity from human fetal membranes upon exposure to PGE/sub 2/, oxytocin and isoproterenol

    Energy Technology Data Exchange (ETDEWEB)

    Poisner, A.M.; Poisner, R.; Becca, C.R.; Conn, P.M.

    1986-03-01

    The authors have previously reported that superfused chorion laeve (fetal membranes) release LHRH-like immunoreactivity upon exposure to angiotensin II. They have now studied the effects of other agonists on the release of LHRH-activity and something of its chemical nature. Fetal membranes were obtained from placentas delivered by cesarean section, the amnion stripped from the chorion, and the chorion superfused in an Amicon thin-channel device with the maternal surface facing up. The whole device was submerged in a 37 C water bath and perfused with a modified Locke's solution at 0.4 - 1.0 ml/min. LHRH-activity was measured by radioimmunoassay using three different antisera against LHRH. The release of LHRH-activity was stimulated by 6-10 min exposure to PGE/sub 2/, oxytocin, and isoproterenol. Extracts of chorion were studied using gel filtration on Sephacryl S-200 and ultrafiltration with Amicon PM-10 filters. The bulk of the LHRH-activity appeared as a higher molecular weight form (about 70,000 daltons). Since oxytocin has been reported to release PGE/sub 2/ from chorion, it may release LHRH-activity by virtue of liberating endogenous PGE/sub 2/. The chemical nature of the LHRH-activity is presently under investigation.

  13. Human pituitary and placental hormones control human insulin-like growth factor II secretion in human granulosa cells

    Energy Technology Data Exchange (ETDEWEB)

    Ramasharma, K.; Li, C.H.

    1987-05-01

    Human granulosa cells cultured with calf serum actively proliferated for 18-20 generations and secreted progesterone into the medium; progesterone levels appeared to decline with increase in generation number. Cells cultured under serum-free conditions secreted significant amounts of progesterone and insulin-like growth factor II (IGF-II). The progesterone secretion was enhanced by the addition of human follitropin, lutropin, and chorionic gonadotropin but not by growth hormone. These cells, when challenged to varying concentrations of human growth hormone, human chorionic somatomammotropin, human prolactin, chorionic gonadotropin, follitropin, and lutropin, secreted IGF-II into the medium as measured by specific IGF-II RIA. Among these human hormones, chorionic gonadotropin, follitropin, and lutropin were most effective in inducing IGF-II secretion from these cells. When synthetic lutropin-releasing hormone and ..cap alpha..-inhibin-92 were tested, only lutropin-releasing hormone was effective in releasing IGF-II. The results described suggest that cultured human granulosa cells can proliferate and actively secrete progesterone and IGF-II into the medium. IGF-II production in human granulosa cells was influenced by a multi-hormonal complex including human growth hormone, human chorionic somatomammotropin, and prolactin.

  14. Human pituitary and placental hormones control human insulin-like growth factor II secretion in human granulosa cells

    International Nuclear Information System (INIS)

    Human granulosa cells cultured with calf serum actively proliferated for 18-20 generations and secreted progesterone into the medium; progesterone levels appeared to decline with increase in generation number. Cells cultured under serum-free conditions secreted significant amounts of progesterone and insulin-like growth factor II (IGF-II). The progesterone secretion was enhanced by the addition of human follitropin, lutropin, and chorionic gonadotropin but not by growth hormone. These cells, when challenged to varying concentrations of human growth hormone, human chorionic somatomammotropin, human prolactin, chorionic gonadotropin, follitropin, and lutropin, secreted IGF-II into the medium as measured by specific IGF-II RIA. Among these human hormones, chorionic gonadotropin, follitropin, and lutropin were most effective in inducing IGF-II secretion from these cells. When synthetic lutropin-releasing hormone and α-inhibin-92 were tested, only lutropin-releasing hormone was effective in releasing IGF-II. The results described suggest that cultured human granulosa cells can proliferate and actively secrete progesterone and IGF-II into the medium. IGF-II production in human granulosa cells was influenced by a multi-hormonal complex including human growth hormone, human chorionic somatomammotropin, and prolactin

  15. Modern Technologies In Ectopic Pregnancy Diagnostics On Hospital Stage

    Directory of Open Access Journals (Sweden)

    L.V. Kaushanskaya

    2009-12-01

    Full Text Available The present research provides analysis of results of preoperative examination of 680 patients with ectopic pregnancy depending on the range of surgical treatment. It has been shown that in case of progressive ectopic pregnancy the diagnostic significance of concentrations of human chorionic gonadotropin, transvaginal examination and laparos-copy depends on the duration of pregnancy. When the term of ectopic pregnancy is 3-4 weeks monitoring of p chorionic gonadotropin in blood serum (99.5%, transvaginal examination (58% and laparoscopy (78.5% are more informative. When the term of ectopic pregnancy is more than 4 weeks there is a high diagnostic value of monitoring p-subunit of chorionic gonadotropin (99.5%, transvaginal examination (68% and laparoscopy (99,5%. The research has proved that pregnancy period of 3-4 weeks is optimal for laparoscopy and other operations

  16. Impact of a new national screening policy for Down's syndrome in Denmark

    DEFF Research Database (Denmark)

    Ekelund, Charlotte K; Jørgensen, Finn Stener; Petersen, Olav Bjørn;

    2008-01-01

    OBJECTIVES: To evaluate the impact of a screening strategy in the first trimester, introduced in Denmark during 2004-6, on the number of infants born with Down's syndrome and the number of chorionic villus samplings and amniocenteses, and to determine detection and false positive rates...... and newborn infants with Down's syndrome diagnosed prenatally and postnatally and number of chorionic villus samplings and amniocenteses carried out. Secondary outcomes measured were number of women screened in 2005 and 2006, screen positive rate, and information on screening in 2005 and 2006 for infants...... with a postnatal diagnosis of Down's syndrome. RESULTS: The number of infants born with Down's syndrome decreased from 55-65 per year during 2000-4 to 31 in 2005 and 32 in 2006. The total number of chorionic villus samplings and amniocenteses carried out decreased from 7524 in 2000 to 3510 in 2006. The detection...

  17. Hypothetical role of prostaglandins in the onset of preterm labor after fetal surgery.

    Science.gov (United States)

    Pomini, Francesco; Noia, Giuseppe; Mancuso, Salvatore

    2007-01-01

    Preterm labor is one of the most important factors limiting the advancement of fetal surgery programs. While prostaglandins (PGs) have long been indicated as the key factor in the initiation of labor in humans, there is significant evidence showing that the chorionic membrane acts as a powerful barrier between the decidua/myometrium and amniotic PGs during normal pregnancy. After either open or endoscopic fetal surgery the imperfect, non-hermetical closure of the chorion permits leakage of PGs from the amnionic sac, allowing them to reach the decidua and myometrium. The surgical wound in the chorionic barrier could be the major factor involved in preterm labor and delivery after human fetal surgery. PMID:17135752

  18. Multipotent stromal stem cells from human placenta demonstrate high therapeutic potential.

    Science.gov (United States)

    Nazarov, Igor; Lee, Jae W; Soupene, Eric; Etemad, Sara; Knapik, Derrick; Green, William; Bashkirova, Elizaveta; Fang, Xiaohui; Matthay, Michael A; Kuypers, Frans A; Serikov, Vladimir B

    2012-05-01

    We describe human chorionic mesenchymal stem cell (hCMSC) lines obtained from the chorion of human term placenta with high therapeutic potential in human organ pathology. hCMSCs propagated for more than 100 doublings without a decrease in telomere length and with no telomerase activity. Cells were highly positive for the embryonic stem cell markers OCT-4, NANOG, SSEA-3, and TRA-1-60. In vitro, cells could be differentiated into neuron-like cells (ectoderm), adipocytes, osteoblasts, endothelial-like cells (mesoderm), and hepatocytes (endoderm)-derivatives of all three germ layers. hCMSCs effectively facilitated repair of injured epithelium as demonstrated in an ex vivo-perfused human lung preparation injured by Escherichia coli endotoxin and in in vitro human lung epithelial cultures. We conclude that the chorion of human term placenta is an abundant source of multipotent stem cells that are promising candidates for cell-based therapies. PMID:23197815

  19. Impact of a new national screening policy for Down's syndrome in Denmark: population based cohort study

    DEFF Research Database (Denmark)

    Ekelund, Charlotte Kvist; Petersen, O.B.; Sundberg, K.;

    2008-01-01

    OBJECTIVES: To evaluate the impact of a screening strategy in the first trimester, introduced in Denmark during 2004-6, on the number of infants born with Down's syndrome and the number of chorionic villus samplings and amniocenteses, and to determine detection and false positive rates in the...... newborn infants with Down's syndrome diagnosed prenatally and postnatally and number of chorionic villus samplings and amniocenteses carried out. Secondary outcomes measured were number of women screened in 2005 and 2006, screen positive rate, and information on screening in 2005 and 2006 for infants with...... a postnatal diagnosis of Down's syndrome. RESULTS: The number of infants born with Down's syndrome decreased from 55-65 per year during 2000-4 to 31 in 2005 and 32 in 2006. The total number of chorionic villus samplings and amniocenteses carried out decreased from 7524 in 2000 to 3510 in 2006. The...

  20. A re-assessment of biochemical marker distributions in T21 affected and unaffected twin pregnancies in the first trimester

    DEFF Research Database (Denmark)

    Madsen, Helen Nordahl; Tørring, Niels

    unaffected and 47 trisomy 21 affected twin pregnancies were included in the study. Chorionicity specific medians were generated for PAPP-A and free β-hCG from gestational age 8 to 14 weeks. Multiple of the median for each of the markers were calculated. Detection rates (DR) and false-positive rates (FPR....... Allowing for gestation and chorionicity, twin pregnancies affected with trisomy 21 had higher levels of free β-hCG and lower levels of PAPP-A. Adding biochemistry into the risk assessment increased the DR for fetal trisomy 21 in dizygotic twin pregnancies from 78% to 90%, and decreased the FPR from 8...... biochemical markers pregnancy-associated plasma protein-A (PAPP-A) and free β-human chorionic gonadotropin (free β-hCG) in twin pregnancies relative to singleton pregnancies and establish improved screening procedure for chromosomal anomalies such as trisomy 21 in twin pregnancies. METHODS A total of 4843...

  1. Impact of a new national screening policy for Down's syndrome in Denmark: population based cohort study

    DEFF Research Database (Denmark)

    Ekelund, Charlotte K; Jørgensen, Finn Stener; Petersen, Olav Bjørn;

    2008-01-01

    OBJECTIVES: To evaluate the impact of a screening strategy in the first trimester, introduced in Denmark during 2004-6, on the number of infants born with Down's syndrome and the number of chorionic villus samplings and amniocenteses, and to determine detection and false positive rates...... and newborn infants with Down's syndrome diagnosed prenatally and postnatally and number of chorionic villus samplings and amniocenteses carried out. Secondary outcomes measured were number of women screened in 2005 and 2006, screen positive rate, and information on screening in 2005 and 2006 for infants...... with a postnatal diagnosis of Down's syndrome. RESULTS: The number of infants born with Down's syndrome decreased from 55-65 per year during 2000-4 to 31 in 2005 and 32 in 2006. The total number of chorionic villus samplings and amniocenteses carried out decreased from 7524 in 2000 to 3510 in 2006. The detection...

  2. Distribution of CA 125 in placental tissues.

    Science.gov (United States)

    Fuith, L C; Müller-Holzner, E; Marth, C; Perkmann, E; Zeimet, A; Daxenbichler, G

    1989-01-01

    The presence of the tumor marker CA 125 was studied in different compartments of the human placenta. Levels of CA 125 in the cytosol of chorionic villi ranged from 27-17100 U/g (median 560 U/g). In the placental amnion and chorion concentrations ranged from 175-29000 U/g, median 1060 U/g and were not statistically different. In the umbilical cord values were significantly lower (range 44-7600 U/g; median 180 U/g). Maternal serum probes were above the upper limit of normal in all cases (range 48-500 U/ml; median 131 U/ml). Immunohistochemistry detected CA 125 exclusively within the amniotic cells of the placenta and the umbilical cord. This might be because CA 125 fixes more to insoluble structures in the amnion or because of contamination of chorionic villi with the underlying decidua.

  3. Familial functional anorchism: a review of etiology and management.

    Science.gov (United States)

    Connors, M H; Styne, D M

    1985-06-01

    Identical male twins with small penes and bilateral unpalpable gonads were unresponsive to human chorionic gonadotropin stimulation. Both infants had elevated levels of gonadotropins. The size of the penis did not meet fully the criteria for micropenis and the organ was responsive to testosterone therapy. The use of primary human chorionic gonadotropin stimulation followed by testosterone measurements is indicated for children with cryptorchidism in whom the etiology of micropenis is in doubt. We report the first observation of anorchism in identical twins. PMID:3999209

  4. NCBI nr-aa BLAST: CBRC-OLAT-15-0021 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OLAT-15-0021 ref|NP_037110.1| luteinizing hormone/choriogonadotropin receptor ...[Rattus norvegicus] sp|P16235|LSHR_RAT Lutropin-choriogonadotropic hormone receptor precursor (LH/CG-R) (LSH-R) (Lute...inizing hormone receptor) pir||I77463 luteinizing hormone/chorionic gonadotropin receptor - rat gb|...AAA41528.1| choriogonadotropic hormone receptor gb|AAA41529.1| luteinizing hormon...e receptor gb|AAB22680.1| luteinizing hormone/chorionic gonadotropin receptor; LH/CG receptor [Rattus sp.] gb|EDM02617.1| lute

  5. NCBI nr-aa BLAST: CBRC-GGAL-03-0008 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GGAL-03-0008 ref|NP_037110.1| luteinizing hormone/choriogonadotropin receptor ...[Rattus norvegicus] sp|P16235|LSHR_RAT Lutropin-choriogonadotropic hormone receptor precursor (LH/CG-R) (LSH-R) (Lute...inizing hormone receptor) pir||I77463 luteinizing hormone/chorionic gonadotropin receptor - rat gb|...AAA41528.1| choriogonadotropic hormone receptor gb|AAA41529.1| luteinizing hormon...e receptor gb|AAB22680.1| luteinizing hormone/chorionic gonadotropin receptor; LH/CG receptor [Rattus sp.] gb|EDM02617.1| lute

  6. NCBI nr-aa BLAST: CBRC-TGUT-05-0004 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TGUT-05-0004 ref|NP_037110.1| luteinizing hormone/choriogonadotropin receptor ...[Rattus norvegicus] sp|P16235|LSHR_RAT Lutropin-choriogonadotropic hormone receptor precursor (LH/CG-R) (LSH-R) (Lute...inizing hormone receptor) pir||I77463 luteinizing hormone/chorionic gonadotropin receptor - rat gb|...AAA41528.1| choriogonadotropic hormone receptor gb|AAA41529.1| luteinizing hormon...e receptor gb|AAB22680.1| luteinizing hormone/chorionic gonadotropin receptor; LH/CG receptor [Rattus sp.] gb|EDM02617.1| lute

  7. NCBI nr-aa BLAST: CBRC-LAFR-01-3395 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-LAFR-01-3395 ref|NP_037110.1| luteinizing hormone/choriogonadotropin receptor ...[Rattus norvegicus] sp|P16235|LSHR_RAT Lutropin-choriogonadotropic hormone receptor precursor (LH/CG-R) (LSH-R) (Lute...inizing hormone receptor) pir||I77463 luteinizing hormone/chorionic gonadotropin receptor - rat gb|...AAA41528.1| choriogonadotropic hormone receptor gb|AAA41529.1| luteinizing hormon...e receptor gb|AAB22680.1| luteinizing hormone/chorionic gonadotropin receptor; LH/CG receptor [Rattus sp.] gb|EDM02617.1| lute

  8. NCBI nr-aa BLAST: CBRC-ETEL-01-0424 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ETEL-01-0424 ref|NP_037110.1| luteinizing hormone/choriogonadotropin receptor ...[Rattus norvegicus] sp|P16235|LSHR_RAT Lutropin-choriogonadotropic hormone receptor precursor (LH/CG-R) (LSH-R) (Lute...inizing hormone receptor) pir||I77463 luteinizing hormone/chorionic gonadotropin receptor - rat gb|...AAA41528.1| choriogonadotropic hormone receptor gb|AAA41529.1| luteinizing hormon...e receptor gb|AAB22680.1| luteinizing hormone/chorionic gonadotropin receptor; LH/CG receptor [Rattus sp.] gb|EDM02617.1| lute

  9. NCBI nr-aa BLAST: CBRC-MMUS-17-0192 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-17-0192 ref|NP_037110.1| luteinizing hormone/choriogonadotropin receptor ...[Rattus norvegicus] sp|P16235|LSHR_RAT Lutropin-choriogonadotropic hormone receptor precursor (LH/CG-R) (LSH-R) (Lute...inizing hormone receptor) pir||I77463 luteinizing hormone/chorionic gonadotropin receptor - rat gb|...AAA41528.1| choriogonadotropic hormone receptor gb|AAA41529.1| luteinizing hormon...e receptor gb|AAB22680.1| luteinizing hormone/chorionic gonadotropin receptor; LH/CG receptor [Rattus sp.] gb|EDM02617.1| lute

  10. NCBI nr-aa BLAST: CBRC-OANA-01-1570 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OANA-01-1570 ref|NP_037110.1| luteinizing hormone/choriogonadotropin receptor ...[Rattus norvegicus] sp|P16235|LSHR_RAT Lutropin-choriogonadotropic hormone receptor precursor (LH/CG-R) (LSH-R) (Lute...inizing hormone receptor) pir||I77463 luteinizing hormone/chorionic gonadotropin receptor - rat gb|...AAA41528.1| choriogonadotropic hormone receptor gb|AAA41529.1| luteinizing hormon...e receptor gb|AAB22680.1| luteinizing hormone/chorionic gonadotropin receptor; LH/CG receptor [Rattus sp.] gb|EDM02617.1| lute

  11. NCBI nr-aa BLAST: CBRC-CFAM-10-0034 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CFAM-10-0034 ref|NP_037110.1| luteinizing hormone/choriogonadotropin receptor ...[Rattus norvegicus] sp|P16235|LSHR_RAT Lutropin-choriogonadotropic hormone receptor precursor (LH/CG-R) (LSH-R) (Lute...inizing hormone receptor) pir||I77463 luteinizing hormone/chorionic gonadotropin receptor - rat gb|...AAA41528.1| choriogonadotropic hormone receptor gb|AAA41529.1| luteinizing hormon...e receptor gb|AAB22680.1| luteinizing hormone/chorionic gonadotropin receptor; LH/CG receptor [Rattus sp.] gb|EDM02617.1| lute

  12. NCBI nr-aa BLAST: CBRC-FRUB-02-0389 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FRUB-02-0389 ref|NP_037110.1| luteinizing hormone/choriogonadotropin receptor ...[Rattus norvegicus] sp|P16235|LSHR_RAT Lutropin-choriogonadotropic hormone receptor precursor (LH/CG-R) (LSH-R) (Lute...inizing hormone receptor) pir||I77463 luteinizing hormone/chorionic gonadotropin receptor - rat gb|...AAA41528.1| choriogonadotropic hormone receptor gb|AAA41529.1| luteinizing hormon...e receptor gb|AAB22680.1| luteinizing hormone/chorionic gonadotropin receptor; LH/CG receptor [Rattus sp.] gb|EDM02617.1| lute

  13. NCBI nr-aa BLAST: CBRC-RNOR-06-0010 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-06-0010 ref|NP_037110.1| luteinizing hormone/choriogonadotropin receptor ...[Rattus norvegicus] sp|P16235|LSHR_RAT Lutropin-choriogonadotropic hormone receptor precursor (LH/CG-R) (LSH-R) (Lute...inizing hormone receptor) pir||I77463 luteinizing hormone/chorionic gonadotropin receptor - rat gb|...AAA41528.1| choriogonadotropic hormone receptor gb|AAA41529.1| luteinizing hormon...e receptor gb|AAB22680.1| luteinizing hormone/chorionic gonadotropin receptor; LH/CG receptor [Rattus sp.] gb|EDM02617.1| lute

  14. NCBI nr-aa BLAST: CBRC-GACU-06-0009 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GACU-06-0009 ref|NP_037110.1| luteinizing hormone/choriogonadotropin receptor ...[Rattus norvegicus] sp|P16235|LSHR_RAT Lutropin-choriogonadotropic hormone receptor precursor (LH/CG-R) (LSH-R) (Lute...inizing hormone receptor) pir||I77463 luteinizing hormone/chorionic gonadotropin receptor - rat gb|...AAA41528.1| choriogonadotropic hormone receptor gb|AAA41529.1| luteinizing hormon...e receptor gb|AAB22680.1| luteinizing hormone/chorionic gonadotropin receptor; LH/CG receptor [Rattus sp.] gb|EDM02617.1| lute

  15. Are there characteristics of the false-negative cases from the first trimester combined screening programme for Down syndrome?

    DEFF Research Database (Denmark)

    Hartwig, Tanja Schlaikjær; Sørensen, Steen; Jørgensen, Finn Stener

    2014-01-01

    based on screening results of false-negative cases. Screening results from false-negative cases show that maternal age is lower, nuchal translucency smaller, pregnancy-associated plasma protein-A level higher, β-human chorionic gonadotropin level lower and crown-rump length bigger than among true...... of false-negative cases tend toward the values of unaffected pregnancies with lower maternal age, smaller nuchal translucency, higher pregnancy-associated plasma protein-A level, lower β-human chorionic gonadotropin level and bigger crown-rump length than among true positive cases....

  16. Longer time-to-pregnancy in spontaneously conceived pregnancies is associated with lower PAPP-A and free β-hCG in first trimester screening for Down syndrome

    DEFF Research Database (Denmark)

    Kirkegaard, I.; Uldbjerg, N.; Tabor, Ann;

    2014-01-01

    OBJECTIVE: The aim of this study was to investigate whether subfertility, measured as longer time-to-pregnancy (TTP) in spontaneously conceived pregnancies, affects the first trimester levels of pregnancy-associated plasma protein-A (PAPP-A) and free beta-human chorionic gonadotrophin (β-hCG) and......OBJECTIVE: The aim of this study was to investigate whether subfertility, measured as longer time-to-pregnancy (TTP) in spontaneously conceived pregnancies, affects the first trimester levels of pregnancy-associated plasma protein-A (PAPP-A) and free beta-human chorionic gonadotrophin (β...

  17. First-trimester risk calculation for trisomy 13, 18, and 21: comparison of the screening efficiency between 2 locally developed programs and commercial software

    DEFF Research Database (Denmark)

    Sørensen, Steen; Momsen, Günther; Sundberg, Karin;

    2011-01-01

    Reliable individual risk calculation for trisomy (T) 13, 18, and 21 in first-trimester screening depends on good estimates of the medians for fetal nuchal translucency thickness (NT), free β-subunit of human chorionic gonadotropin (hCGβ), and pregnancy-associated plasma protein-A (PAPP-A) in mate......Reliable individual risk calculation for trisomy (T) 13, 18, and 21 in first-trimester screening depends on good estimates of the medians for fetal nuchal translucency thickness (NT), free β-subunit of human chorionic gonadotropin (hCGβ), and pregnancy-associated plasma protein-A (PAPP...

  18. First-trimester risk calculation for trisomy 13, 18, and 21: comparison of the screening efficiency between 2 locally developed programs and commercial software

    DEFF Research Database (Denmark)

    Sørensen, Steen; Momsen, Günther; Sundberg, Karin;

    2011-01-01

    Reliable individual risk calculation for trisomy (T) 13, 18, and 21 in first-trimester screening depends on good estimates of the medians for fetal nuchal translucency thickness (NT), free ß-subunit of human chorionic gonadotropin (hCGß), and pregnancy-associated plasma protein-A (PAPP-A) in mate......Reliable individual risk calculation for trisomy (T) 13, 18, and 21 in first-trimester screening depends on good estimates of the medians for fetal nuchal translucency thickness (NT), free ß-subunit of human chorionic gonadotropin (hCGß), and pregnancy-associated plasma protein-A (PAPP...

  19. Determination of optical properties of normal and adenomatous human colon tissues in vitro using integrating sphere techniques

    Institute of Scientific and Technical Information of China (English)

    Hua-Jiang Wei; Da Xing; Jian-Jun Lu; Huai-Min Gu; Guo-Yong Wu; Ying Jin

    2005-01-01

    AIM: The purpose of the present study is to compare the optical properties of normal human colon mucosa/submucosa and muscle layer/chorion, and adenomatous human colon mucosa/submucosa and muscle layer/chorion in vitro at 476.5, 488, 496.5, 514.5 and 532 nm. We believe these differences in optical properties should help differential diagnosis of human colon tissues by using optical methods.METHODS: In vitro optical properties were investigated for four kinds of tissues: normal human colon mucosa/submucosa and muscle layer/chorion, and adenomatous human colon mucosa/submucosa and muscle layer/chorion. Tissue samples were taken from 13 human colons (13 adenomatous, 13 normal). From the normal human colons a total of 26 tissue samples, with a mean thickness of 0.40 mm, were used (13 from mucosa/submucosa and 13 from muscle layer/chorion), and from the adenomatous human bladders a total of 26 tissue samples, with a mean thickness of 0.40 mm, were used (13 from mucosa/submucosa and 13 from muscle layer/chorion). The measurements were performed using a double-integratingsphere setup and the optical properties were assessed from these measurements using the adding-doubling method that was considered reliable.RESULTS: The results of measurement showed that there were significant differences in the absorption coefficients and scattering coefficients between normal and adenomatous human colon mucosa/submucosa at the same wavelength,and there were also significant differences in the two optical parameters between both colon muscle layer/chorion at the same wavelength. And there were large differences in the anisotropy factors between both colon mucosa/submucosa at the same wavelength, there were also large differences in the anisotropy factors between both colon muscle layer/chorion at the same wavelength.There were large differences in the value ranges of the absorption coefficients, scattering coefficients and anisotropy factors between both colon mucosa/submucosa,and there

  20. High macrosomia rate in healthy fetuses after enlarged nuchal translucency

    NARCIS (Netherlands)

    Timmerman, E.; Pajkrt, E.; Snijders, R. J. M.; Bilardo, C. M.

    2014-01-01

    ObjectiveThe aim of this study was to investigate the association of the first trimester screening variables nuchal translucency (NT), pregnancy associated plasma protein (PAPP-A), and free beta-human chorionic gonadotrophin (hCG) and birth weight, with a focus on the prediction of macrosomia. Metho

  1. Autism Spectrum Disorder Risk in Relation to Maternal Mid-Pregnancy Serum Hormone and Protein Markers from Prenatal Screening in California

    Science.gov (United States)

    Windham, Gayle C.; Lyall, Kristen; Anderson, Meredith; Kharrazi, Martin

    2016-01-01

    We examined prenatal screening markers and offspring autism spectrum disorder (ASD) using California statewide data on singleton births in 1996 and 2002. Second trimester levels of unconjugated estriol (uE3), human chorionic gonadotropin (hCG), and maternal serum alpha-fetoprotein (MSAFP) were compared between mothers of children with ASD…

  2. EFFECT OF TAMOXIFEN ON THE SECRETORY FUNCTION OF HUMAN TROPHOBLASTIC CELLS

    Institute of Scientific and Technical Information of China (English)

    HANGui-Zhen; CHUYun-Hong; ZHOUYu-Fen

    1989-01-01

    Gu et al observed the morphological damages and the suppressed secretion of HCG in vitro cultured human placental chorionic tissues caused by tamoxifen (20ug/ml).TarnoxiFeu blood concentrations, during oral administration. 20rag, b.i.d, in early pregnant

  3. Is routine karyotyping required in prenatal samples with a molecular or metabolic referral?

    NARCIS (Netherlands)

    Kooper, A.J.A.; Pieters, J.J.; Faas, B.H.W.; Hoefsloot, L.H.; Burgt, C.J.A.M. van der; Zondervan, H.A.; Smits, A.P.T.

    2012-01-01

    ABSTRACT: As a routine, karyotyping of invasive prenatal samples is performed as an adjunct to referrals for DNA mutation detection and metabolic testing. We performed a retrospective study on 500 samples to assess the diagnostic value of this procedure. These samples included 454 (90.8%) chorionic

  4. Effects of growth hormone, prolactin, and placental lactogen on insulin content and release, and deoxyribonucleic acid synthesis in cultured pancreatic islets

    DEFF Research Database (Denmark)

    Nielsen, Jens Høiriis

    1982-01-01

    The direct effects of human GH (hGH), ovine pituitary PRL (oPRL), and human chorionic somatomammotropin [placental lactogen (hPL)] on the endocrine pancreas were studied in isolated pancreatic islets maintained in tissue culture. Islets of Langerhans were isolated by collagenase treatment of panc...

  5. Screening in early pregnancy : more than Down syndrome alone

    NARCIS (Netherlands)

    Wortelboer, E.J.

    2010-01-01

    Abstract Since 2004 it was allowed to inform all pregnant women on the possibility of having a screening test for DS, with the first-trimester combined test (maternal serum concentrations of pregnancy-associated plasma protein A (PAPP-A) and the free beta subunit of human chorion gonadotrophin (f?-h

  6. Ultrastructure of Proechinophthirus zumpti (Anoplura, Echinophthiriidae by scanning electron microscopy

    Directory of Open Access Journals (Sweden)

    Dolores del Carmen Castro

    2002-09-01

    Full Text Available The ultrastructure of Proechinophthirus zumpti Werneck, 1955, mainly the external chorionic features of the egg, is described through electronic microscopy techniques. This species was first cited in Argentina, infesting Arctocephalus australis (Zimmermann, 1873. The morphological adaptations of adults and nymphs are described in both species of Proechinophthirus parasitic on Otariidae: P. fluctus (Ferris, 1916 and P. zumpti.

  7. Improved first-trimester Down syndrome screening performance by lowering the false-positive rate: a prospective study of 9941 low-risk women

    DEFF Research Database (Denmark)

    Wøjdemann, K R; Shalmi, A C; Christiansen, M;

    2005-01-01

    OBJECTIVE: To determine the performance of screening for Down syndrome (DS) and other major chromosomal abnormalities using nuchal translucency (NT), free beta-human chorionic gonadotropin (beta-hCG) and pregnancy-associated plasma protein-A (PAPP-A) in a prospective study of a non...

  8. Risk of fetal loss associated with invasive testing following combined first-trimester screening for Down syndrome

    DEFF Research Database (Denmark)

    Wulff, C B; Gerds, T A; Rode, L;

    2016-01-01

    OBJECTIVE: To assess prospectively the risk of fetal loss associated with chorionic villus sampling (CVS) and amniocentesis (AC) following combined first-trimester screening (cFTS) for Down syndrome. METHODS: This was a nationwide population-based study (Danish Fetal Medicine Database, 2008...

  9. Optimizing heterologous expression in Dictyostelium : importance of 5 ' codon adaptation

    NARCIS (Netherlands)

    Vervoort, EB; van Ravestein, A; van Peij, NNME; Heikoop, JC; van Haastert, OJM; Verheijden, GF; Linskens, MHK; Heikoop, Judith C.; Haastert, Peter J.M. van; Verheijden, Gijs F.

    2000-01-01

    Expression of heterologous proteins in Dictyostelium discoideum presents unique research opportunities, such as the functional analysis of complex human glycoproteins after random mutagenesis, In one study, human chorionic gonadotropin (hCG) and human follicle stimulating hormone were expressed in D

  10. A new marker set that identifies fetal cells in maternal circulation with high specificity

    DEFF Research Database (Denmark)

    Hatt, Lotte; Brinch, Marie; Singh, Ripudaman;

    2014-01-01

    OBJECTIVE: Fetal cells from the maternal circulation (FCMBs) have the potential to replace cells from amniotic fluid or chorionic villi in a diagnosis of common chromosomal aneuploidies. Good markers for enrichment and identification are lacking. METHOD: Blood samples from 78 normal pregnancies...

  11. A prospective study evaluating the performance of first trimester combined screening for trisomy 21 using repeated sampling of the maternal serum markers PAPP-A and free β-hCG

    DEFF Research Database (Denmark)

    Ekelund, Charlotte Kvist; Wright, Dave; Ball, Susan;

    2012-01-01

    To prospectively evaluate the performance of first-trimester combined screening for trisomy 21 using the biochemical markers pregnancy-associated plasma protein-A (PAPP-A) and free beta-human chorionic gonadotropin (free β-hCG) obtained before and at the time of the nuchal translucency (NT) scan....

  12. Temporal effects of maternal and pregnancy characteristics on serum PAPP-A and free β-hCG at 7-14 weeks' gestation

    DEFF Research Database (Denmark)

    Ball, Susan; Ekelund, Charlotte; Wright, Dave;

    2013-01-01

    Objective: Investigate gestational age dependent effects of racial origin, smoking status and mode of conception on maternal serum levels of free ß-human chorionic gonadotropin (β-hCG) and pregnancy associated plasma protein-A (PAPP-A) at 7-14 weeks' gestation. Methods: Data arise from prospective...

  13. Gonadotropin-releasing hormone agonist versus HCG for oocyte triggering in antagonist assisted reproductive technology cycles

    NARCIS (Netherlands)

    M.A.F.M. Youssef; F. van der Veen; H.G. Al-Inany; G. Griesinger; M.H. Mochtar; M. van Wely

    2010-01-01

    Background Gonadotropin-releasing hormone (GnRH) antagonist protocols for pituitary down regulation in in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) allow the use of GnRH agonists for triggering final oocyte maturation. Currently, human chorionic gonadotropin (HCG) is stil

  14. Invasive Mole Presenting as Acute Haemoperitoneum

    Directory of Open Access Journals (Sweden)

    Sunesh Kumar, N. Vimala, Suneeta Mittal

    2004-07-01

    Full Text Available We report a case of invasive hydatidiform mole presenting as an acute primary haemoperitoneum.The patient presented with acute abdominal pain and signs of haemoperitoneum. Emergencylaparotomy revealed a molar pregnancy perforating through the uterine fundus, resulting in massivehaemoperitoneum. The serum beta chorionic gonado-tropin (ß-hCG levels regressed spontaneouslyfollowing evacuation of the molar pregnancy.

  15. Aborting a Malformed Fetus: A Debatable Issue in Saudi Arabia

    OpenAIRE

    Al-Alaiyan, Saleh; Khalid M AlFaleh

    2012-01-01

    Congenital anomalies contribute a significant proportion of infant morbidity and mortality, as well as fetal mortality. They are generally grouped into three major categories: structural/metabolic, congenital infections, and other conditions. The most prevalent conditions include congenital heart defects, orofacial clefts, Down syndrome, and neural tube defects. Several prenatal diagnostic procedures have been introduced, both cytogenetic (such as chorion biopsy, amniocentesis and funiculocen...

  16. First trimester combined screening - focus on early biochemistry

    DEFF Research Database (Denmark)

    Tørring, Niels

    2016-01-01

    First trimester combined screening (cFTS) for foetal trisomy 21 has become an established method in many countries. The screening is based on a combination of maternal-age-related risk, ultrasound (nuchal translucency) and two maternal serum biochemical markers, free beta human chorionic gonadotr...

  17. Noninvasive prenatal detection of genetic defects

    NARCIS (Netherlands)

    Oever, Jessica Maria Elisabeth van den

    2016-01-01

    Current prenatal diagnostics is mainly based on obtaining fetal DNA through invasive procedures such as chorionic villi sampling (CVS) or amniocentesis. These procedures are associated with a small, but significant risk of fetal loss. The discovery of the presence of cell-free fetal DNA (cffDNA) in

  18. Cell-free fetal DNA in the maternal circulation originates from the cytotrophoblast : proof from an unique case

    NARCIS (Netherlands)

    Hochstenbach, Ron; Nikkels, Peter G J; Elferink, Martin G; Oudijk, Martijn A; van Oppen, Carla; van Zon, Patrick; van Harssel, Jeske; Schuring-Blom, Heleen; Page-Christiaens, Godelieve C M L

    2015-01-01

    Noninvasive prenatal testing (NIPT) and direct karyotyping of cytotrophoblast were normal for a male fetus, but cultured chorionic villus mesenchymal cells and umbilical cord fibroblasts showed nonmosaic trisomy 18. This observation provides direct evidence for the cytotrophoblastic origin of cell-f

  19. Inhibition of diabetes in NOD mice by human pregnancy factor

    NARCIS (Netherlands)

    Khan, N.A.; Khan, A.; Savelkoul, H.F.J.; Benner, R.

    2001-01-01

    Clinical symptoms of Th1 mediated autoimmune diseases regress in many patients during pregnancy. A prominent feature of pregnancy is the presence of human chorionic gonadotrophin hormone (hCG) in blood and urine. In this report we tested the effect of clinical grade hCG (c-hCG) on the development of

  20. Prenatal diagnosis of autosomal dominant hereditary spastic paraplegia (SPG4) using direct mutation detection

    DEFF Research Database (Denmark)

    Nielsen, Jørgen E; Koefoed, Pernille; Kjaergaard, Susanne;

    2004-01-01

    individuals, the affected father, the mother, and fetal DNA from an ongoing pregnancy by chorionic villus sampling (CVS) in the first trimester. The spastin gene (SPG4) was completely sequenced. RESULTS: A novel 832insGdelAA frameshift mutation, predicted to cause loss of functional protein, was identified...

  1. Development of a preparation and staining method for fetal erythroblasts in maternal blood : Simultaneous immunocytochemical staining and FISH analysis

    NARCIS (Netherlands)

    Oosterwijk, JC; Mesker, WE; Ouwerkerk-van Velzen, MCM; Knepfle, CFHM; Wiesmeijer, KC; van den Burg, MJM; Beverstock, GC; Bernini, LF; van Ommen, Gert-Jan B; Kanhai, HHH; Tanke, HJ

    1998-01-01

    In order to detect fetal nucleated red blood cells (NRBCs) in maternal blood, a protocol was developed which aimed at producing a reliable staining method for combined immunocytochemical and FISH analysis. The technique had to be suitable for eventual automated screening of slides. Chorionic villi w

  2. De Novo-Synthesized Retinoic Acid in Ovarian Antral Follicles Enhances FSH-Mediated Ovarian Follicular Cell Differentiation and Female Fertility.

    Science.gov (United States)

    Kawai, Tomoko; Yanaka, Noriyuki; Richards, JoAnne S; Shimada, Masayuki

    2016-05-01

    Retinoic acid (RA) is the active form of vitamin A and is synthesized from retinol by two key enzymes, alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). As the physiological precursor of RA, retinol impacts female reproductive functions and fertility. The expression of Adh1 and Adh5 as well as Aldh1a1 and Aldh1a7 are significantly increased in the ovaries of mice treated with equine chorionic gonadotropin/FSH. The RA receptor is expressed and localized in granulosa cells and is activated by endogenous RA as indicated by LacZ expression in granulosa cells of RA-responsive transgene-LacZ transgenic mice (RA reporter mice). Coinjection of the ADH inhibitor, 4-methylpyrazole, with equine chorionic gonadotropin significantly decreases the number and developmental competence of oocytes ovulated in response to human chorionic gonadotropin/LH as compared with controls. Injections of RA completely reverse the effects of the inhibitor of ovulation and oocyte development. When mice were fed a retinol-free, vitamin A-deficient diet that significantly reduced the serum levels of retinol, the expression of the LH receptor (Lhcgr) was significantly lower in the ovaries of the vitamin A-deficient mice, and injections of human chorionic gonadotropin failed to induce genes controlling ovulation. These results indicate that ovarian de novo biosynthesis of RA is required for the follicular expression of Lhcgr in granulosa cells and their ability to respond to the ovulatory LH surge. PMID:27022678

  3. Identification of patients with persistent trophoblastic disease after complete hydatidiform mole by using a normal 24-hour urine hCG regression curve

    NARCIS (Netherlands)

    Cromvoirt, S.M. van; Thomas, C.M.G.; Quinn, M.A.; McNally, O.M.; Bekkers, R.L.M.

    2014-01-01

    OBJECTIVE: The aim of this study was to establish a reference 24-hour urine human chorionic gonadotropin (hCG) regression curve in patients with complete hydatidiform mole (CHM) as diagnostic tool in the prediction of persistent trophoblastic disease (PTD). METHODS: From 2004 to 2011, 312 cases suit

  4. Cell-free fetal DNA in the maternal circulation originates from the cytotrophoblast: proof from an unique case

    OpenAIRE

    Hochstenbach, Ron; Nikkels, Peter G. J.; Martin G Elferink; Oudijk, Martijn A; Oppen, Carla; van Zon, Patrick; van Harssel, Jeske; Schuring-Blom, Heleen; Page-Christiaens, Godelieve C M L

    2015-01-01

    Key Clinical Message Noninvasive prenatal testing (NIPT) and direct karyotyping of cytotrophoblast were normal for a male fetus, but cultured chorionic villus mesenchymal cells and umbilical cord fibroblasts showed nonmosaic trisomy 18. This observation provides direct evidence for the cytotrophoblastic origin of cell-free fetal DNA and yields a biological explanation for falsely reassuring NIPT results.

  5. Cell-free fetal DNA in the maternal circulation originates from the cytotrophoblast : proof from an unique case

    OpenAIRE

    Hochstenbach, Ron; Nikkels, Peter G. J.; Martin G Elferink; Oudijk, Martijn A; Oppen, Carla; van Zon, Patrick; van Harssel, Jeske; Schuring-Blom, Heleen; Page-Christiaens, Godelieve C M L

    2015-01-01

    Noninvasive prenatal testing (NIPT) and direct karyotyping of cytotrophoblast were normal for a male fetus, but cultured chorionic villus mesenchymal cells and umbilical cord fibroblasts showed nonmosaic trisomy 18. This observation provides direct evidence for the cytotrophoblastic origin of cell-free fetal DNA and yields a biological explanation for falsely reassuring NIPT results.

  6. Probability distributions for measures of placental shape and morphology

    International Nuclear Information System (INIS)

    Birthweight at delivery is a standard cumulative measure of placental growth, but is a crude summary of other placental characteristics, such as, e.g., the chorionic plate size, and the shape and position of the umbilical cord insertion. Distributions of such measures across a cohort reveal information about the developmental history of the chorionic plate which is unavailable from an analysis based solely on the mean and standard deviation. Various measures were determined from digitized images of chorionic plates obtained from the pregnancy, infection, and nutrition study, a prospective cohort study of preterm birth in central North Carolina between 2002 and 2004. Centroids (geometric centers) and umbilical cord insertions were taken directly from the images. Chorionic plate outlines were obtained from an interpolation based on a Fourier series, while eccentricity (of the best-fit ellipse), skewness, and kurtosis were determined from the method of moments. Histograms of each variable were compared against the normal, lognormal, and Lévy distributions. Only a single measure (eccentricity) followed a normal distribution. All others followed lognormal or ‘heavy-tailed’ distributions for moderate to extreme deviations from the mean, where the relative likelihood far exceeded those of a normal distribution. (paper)

  7. Relapse rates after two versus three consolidation courses of methotrexate in the treatment of low-risk gestational trophoblastic neoplasia.

    NARCIS (Netherlands)

    Lybol, C.; Sweep, F.C.; Harvey, R.; Mitchell, H.; Short, D.; Thomas, C.M.G.; Ottevanger, P.B.; Savage, P.M.; Massuger, L.F.A.G.; Seckl, M.J.

    2012-01-01

    OBJECTIVE: Methotrexate (MTX) alternating with folinic acid is a commonly used treatment regimen for low-risk gestational trophoblastic neoplasia (GTN). In The Netherlands, two courses of MTX are administered after normalization of serum human chorionic gonadotrophin (hCG) levels (consolidation cour

  8. Prenatal diagnosis in women of advanced maternal age

    NARCIS (Netherlands)

    H. Brandenburg (Helen)

    1992-01-01

    textabstractIn this thesis several aspects of prenatal diagnosis in women of advanced maternal age were studied. The effects of the increasing number of elderly gravidas. the lowering of the maternal age at which prenatal diagnosis became accessible and the introduction of chorionic villus sampling,

  9. WISECONDOR: detection of fetal aberrations from shallow sequencing maternal plasma based on a within-sample comparison scheme

    NARCIS (Netherlands)

    Straver, R.; Sistermans, E.A.; Holstege, H.; Visser, A.; Oudejans, C.B.M.; Reinders, M.J.T.

    2013-01-01

    Genetic disorders can be detected by prenatal diagnosis using Chorionic Villus Sampling, but the 1:100 chance to result in miscarriage restricts the use to fetuses that are suspected to have an aberration. Detection of trisomy 21 cases noninvasively is now possible owing to the upswing of next-gener

  10. Immunohistological demonstration of intermediate trophoblast in the diagnosis of uterine versus ectopic pregnancy

    DEFF Research Database (Denmark)

    Sørensen, Flemming Brandt; Marcussen, N; Daugaard, H O;

    1991-01-01

    Immunohistological demonstration of human placental lactogen (hPL) in non-villous, mononuclear intermediate trophoblastic cells may be of routine diagnostic value, when chorionic villi are absent in endometrial curettings from patients suspected of miscarriage of an intrauterine pregnancy. The hi...

  11. Reference ranges and determinants of total hCG levels during pregnancy: the Generation R Study

    NARCIS (Netherlands)

    T.I.M. Korevaar (Tim); E.A.P. Steegers (Eric); Y.B. de Rijke (Yolanda); S. Schalekamp-Timmermans (S.); W. Visser (Willy); A. Hofman (Albert); V.W.V. Jaddoe (Vincent); H.W. Tiemeier (Henning); T.J. Visser (Theo); M. Medici (Marco); R.P. Peeters (Robin)

    2015-01-01

    textabstractHuman chorionic gonadotropin (hCG) is a pregnancy hormone secreted by the placental synctiotrophoblast cell layer that has been linked to fetal growth and various placental, uterine and fetal functions. In order to investigate the effects of hCG on clinical endpoints, knowledge on refere

  12. Twin pregnancy

    DEFF Research Database (Denmark)

    Sperling, Lene; Tabor, A

    2001-01-01

    Determination of chorionicity is one of the most important issues in the management of twin pregnancy. Modern ultrasound equipment has made it possible to accurately assess placentation already in the first trimester with the lambda sign. With regard to prenatal diagnosis, it is important to know...... for clinicians caring for twin pregnancies....

  13. How Do Health Care Providers Diagnose Birth Defects?

    Science.gov (United States)

    ... amniocentesis are Down syndrome and certain types of muscular dystrophy . Because amniocentesis can cause a miscarriage in about 1 out of 200 cases, it is usually only recommended for pregnancies in which the risk of genetic disorders or other problems is high. Chorionic Villus ...

  14. How Do Health Care Providers Diagnose Intellectual & Developmental Disabilities (IDDs)?

    Science.gov (United States)

    ... amniocentesis are Down syndrome and certain types of muscular dystrophy . Because amniocentesis can cause a miscarriage in about 1 out of 200 cases, it is usually only recommended for pregnancies in which the risk of genetic disorders or other problems is high. Chorionic Villus ...

  15. Cell-free fetal DNA in the maternal circulation originates from the cytotrophoblast: proof from an unique case

    Science.gov (United States)

    Hochstenbach, Ron; Nikkels, Peter G J; Elferink, Martin G; Oudijk, Martijn A; van Oppen, Carla; van Zon, Patrick; van Harssel, Jeske; Schuring-Blom, Heleen; Page-Christiaens, Godelieve C M L

    2015-01-01

    Key Clinical Message Noninvasive prenatal testing (NIPT) and direct karyotyping of cytotrophoblast were normal for a male fetus, but cultured chorionic villus mesenchymal cells and umbilical cord fibroblasts showed nonmosaic trisomy 18. This observation provides direct evidence for the cytotrophoblastic origin of cell-free fetal DNA and yields a biological explanation for falsely reassuring NIPT results. PMID:26185654

  16. Hormonal treatment may harm the germ cells in 1 to 3-year-old boys with cryptorchidism

    DEFF Research Database (Denmark)

    Cortes, Dina; Thorup, Jørgen Mogens; Visfeldt, J

    2000-01-01

    Hormonal treatment with human chorionic gonadotropin (HCG) or gonadotropin releasing hormone may be given initially for cryptorchidism. We evaluated whether hormonal treatment is safe for the germ cells in boys with cryptorchidism 1 to 3 years old in whom follicle-stimulating hormone, luteinizing...

  17. Laser capture microdissection coupled with RNA-seq reveals that short cuboidal trophoblast cells of the porcine placenta possess a transcriptome consistent with a migratory cell type

    Science.gov (United States)

    The porcine placenta is classified as epitheliochorial with a diffuse distribution of chorionic villi that do not invade maternal tissues. Thus, the maternal and fetal bloodstreams are separated by six distinct tissue layers. The porcine fetal-maternal interface (FMI) is substantially folded to incr...

  18. Placental Development in Ongoing Pregnancy and Miscarriage

    NARCIS (Netherlands)

    A.D. Reus (Averil)

    2015-01-01

    markdownabstract__Abstract__ In this thesis three-dimensional ultrasound, three-dimensional power Doppler ultrasound, virtual reality and histologic examination of the chorionic villous vascularization were used to investigate early placental development in normal ongoing pregnancy as well as misca

  19. Phototoxicity of TiO2 nanoparticles to zebrafish (Danio rerio) is dependent on life stage

    Science.gov (United States)

    The zebrafish (Danio rerio) embryo has been increasingly used as a model to evaluate toxicity of manufactured nanomaterials. Many studies indicate that the embryo chorion may protect animals from toxic effects of nanomaterials, suggesting that post-hatch life stages may be more s...

  20. Delayed haemorrhage in conservative surgery for ectopic pregnancy.

    Science.gov (United States)

    Rachagan, S P; Neoh, H S

    1990-03-01

    A case of delayed haemorrhage after conservative surgery for ectopic pregnancy is presented. Brief pathophysiology of the condition is presented. The importance of beta-subunit human chorionic gonadotrophin monitoring of the serum in this patient is highlighted. Surgical procedures to prevent this complication are also discussed.

  1. Non-invasive prenatal molecular detection of a fetal point mutation for congenital adrenal hyperplasia using co-amplification at lower denaturation temperature PCR

    Institute of Scientific and Technical Information of China (English)

    DU Juan; ZOU Xin; PAN Yi; LI Shuang-fei; LU Guang-xiu

    2010-01-01

    @@ Conventional prenatal diagnosis relies on invasive chorionic biopsy or amniocentesis, which increases the risk of miscarriage, and is undertaken at 11-20 weeks gestation.1 The discovery of cell-free fetal DNA in maternal plasma has, however, offered a new strategy for non-invasive prenatal diagnosis.2

  2. Maternal risk factors for abnormal placental growth: The national collaborative perinatal project

    Directory of Open Access Journals (Sweden)

    Nicholson Wanda K

    2008-09-01

    Full Text Available Abstract Background Previous studies of maternal risk factors for abnormal placental growth have focused on placental weight and placental ratio as measures of placental growth. We sought to identify maternal risk factors for placental weight and two neglected dimensions of placental growth: placental thickness and chorionic plate area. Methods We conducted an analysis of 24,135 mother-placenta pairs enrolled in the National Collaborative Perinatal Project, a prospective cohort study of pregnancy and child health. We defined growth restriction as th percentile and hypertrophy as > 90th percentile for three placental growth dimensions: placental weight, placental thickness and chorionic plate area. We constructed parallel multinomial logistic regression analyses to identify (a predictors of restricted growth (vs. normal and (b predictors of hypertrophic growth (vs. normal. Results Black race was associated with an increased likelihood of growth restriction for placental weight, thickness and chorionic plate area, but was associated with a reduced likelihood of hypertrophy for these three placental growth dimensions. We observed an increased likelihood of growth restriction for placental weight and chorionic plate area among mothers with hypertensive disease at 24 weeks or beyond. Anemia was associated with a reduced likelihood of growth restriction for placental weight and chorionic plate area. Pre-pregnancy BMI and pregnancy weight gain were associated with a reduced likelihood of growth restriction and an increased likelihood of hypertrophy for all three dimensions of placental growth. Conclusion Maternal risk factors are either associated with placental growth restriction or placental hypertrophy not both. Our findings suggest that the placenta may have compensatory responses to certain maternal risk factors suggesting different underlying biological mechanisms.

  3. GnRH agonist triggering

    DEFF Research Database (Denmark)

    Kol, Shahar; Humaidan, Peter; Al Humaidan, Peter Samir Heskjær

    2013-01-01

    The concept that a bolus of gonadotrophin-releasing hormone agonist (GnRHa) can replace human chorionic gonadotrophin (HCG) as a trigger of final oocyte maturation was introduced several years ago. Recent developments in the area strengthen this premise. GnRHa trigger offers important advantages...... triggering concept should be challenged and that the GnRHa trigger is the way to move forward with thoughtful consideration of the needs, safety and comfort of our patients. Routinely, human chorionic gonadotrophin (HCG) is used to induce ovulation in fertility treatments. This approach deviates...... significantly from physiology and often results in insufficient hormonal support in early pregnancy and in ovarian hyperstimulation syndrome (OHSS). An alternative approach is to use a gonadotrophin-releasing hormone (GnRH) agonist which allows a more physiological trigger of ovulation and, most importantly...

  4. Evolution of placental function in mammals

    DEFF Research Database (Denmark)

    Carter, Anthony Michael

    2012-01-01

    Placenta has a wide range of functions. Some are supported by novel genes that have evolved following gene duplication events while others require acquisition of gene expression by the trophoblast. Although not expressed in the placenta, high-affinity fetal hemoglobins play a key role in placental...... mother to fetus is maintained by expression of classic sugar and amino acid transporters at the trophoblast microvillous and basal membranes. In contrast, placental peptide hormones have arisen largely by gene duplication, yielding for example chorionic gonadotropins from the luteinizing hormone gene...... and placental lactogens from the growth hormone and prolactin genes. There has been a remarkable degree of convergent evolution with placental lactogens emerging separately in the ruminant, rodent, and primate lineages and chorionic gonadotropins evolving separately in equids and higher primates. Finally...

  5. Combined assessment of polymorphisms in the LHCGR and FSHR genes predict chance of pregnancy after in vitro fertilization

    DEFF Research Database (Denmark)

    Lindgren, I; Bååth, M; Uvebrant, K;

    2016-01-01

    STUDY QUESTION: Can gonadotrophin receptor variants separately or in combination, be used for the prediction of pregnancy chances in in vitro fertilization (IVF) trials? SUMMARY ANSWER: The luteinizing hormone/human chorionic gonadotrophin receptor (LHCGR) variant N312S and the follicle-stimulati......STUDY QUESTION: Can gonadotrophin receptor variants separately or in combination, be used for the prediction of pregnancy chances in in vitro fertilization (IVF) trials? SUMMARY ANSWER: The luteinizing hormone/human chorionic gonadotrophin receptor (LHCGR) variant N312S and the follicle...... prior to IVF, it has not been possible to predict neither the pregnancy outcome, nor the response to the hormone with accuracy. If LHCGR and FSHR variants are recognized as biomarkers for chance of pregnancy, more individualized and thereby more efficient treatment modalities can be developed. STUDY...

  6. EXPRESSION OF P53 PROTEIN AND PROLIFERATING CELL NUCLEAR ANTIGEN IN HUMAN GESTATION TROPHOBLASTIC DISEASE

    Institute of Scientific and Technical Information of China (English)

    黄铁军; 王志忠; 方光光; 刘志恒

    2004-01-01

    Objective: To study the relationship between p53 protein, proliferating cell nuclear antigen (PCNA) expression and benign or malignant gestational trophoblastic disease (MGTD). Methods: The histotomic sections of 48 patients with gestational trophoblastic disease and 24 patients of normal chorionic villi were stained using immunohistochemistry. The monoclonal antibodies were used to determine p53 protein and PCNA. Results: The frequency of p53 and PCNA positive expression were significantly different among the chorionic villi of normal pregnancy, hydratidiform mole (HM) and MGTD. But neither p53 nor PCNA has any relation with the clinical staging or metastasis of MGTD. Conclusion: Both P53 and PCNA are valuable in diagnosis of human gestational trophoblastic disease.

  7. Conversion of thyroxine to triiodothyronine and reverse triiodothyronine in human placenta and fetal membranes.

    Science.gov (United States)

    Banovac, K; Bzik, L; Tislarić, D; Sekso, M

    1980-01-01

    Conversion of thyroxine (T4) to 3,5,3'-triiodothyronine (T3) and reverse 3,3',5'-triiodothyronine (rT3) was measured in vitro in human placenta and fetal membranes. T4 (5 micrograms/ml) was incubated in 0.15 mol/l phosphate buffer with tissue homogenates for 2 h at 37 degrees C, and the T3 and rT3 generated were determined in ethanol extract using RIA methods. The placenta and chorion homogenates converted more T4 to T3 than to rT3; the placenta was more active than the chorion. In both tissues the highest converting activity was found in microsomal fractions. PMID:7399397

  8. Sex hormone studies by radioimmunoassay in pregnant and non-pregnant women and in women treated with hormonal contraceptives

    International Nuclear Information System (INIS)

    Blood concentration profiles for follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, testosterone, estradiol, estriol, progesterone, cortisol and sex hormonebinding globulin throughout a menstrual cycle were derived from measurements by radioimmunoassay and related procedures on serial blood samples from 16 normal women as controls. Similar studies were then performed on 9 normal women receiving a low-dose oral contraceptive combination of D-norgestrel and ethynlestradiol. Further studies were performed on 9 out of 16 normal women in whom progestational contraception was carried out with orally administered lynestrenol or intramuscularly administered norethindrone enathate and on 12 normal pregnant women from the 28th to the 38th week of pregnancy. Additional studies embracing chorionic gonadotropin progesterone and 17-hydroxyprogesterone were performed on 10 normal pregnant women from the 6th to the 12th week of pregnancy. Detailed results are presented and their significance discussed

  9. Using Fluorescence in situ Hybridization to Identify DMD/BMD Deletion Carriers

    Institute of Scientific and Technical Information of China (English)

    Ren-li WANG; Yan-ping XIAO; Xiu-rong JIANG

    2003-01-01

    Objective To identify the deletions in Duchenne/Becker muscular dystrophy (DMD/BMD) by using fluorescence in situ hybridization (FISH) Methods The exon-specific cosmid DNA probes (representing 18 exons) were used to perform one-color FISH on metaphase and interphase preparations. The peripheral blood samples from 9 normal people (4 males and 5 females) and 5 females from independent deletion DMD/BMD families, as well as 2 amniotic fluid specimens and 2 chorionic villus samples (CVS) from normal pregnant females were analyzed.Results 72%~100% of peripheral blood lymphocyte metaphases or interphases, 60%~70% of amniocyte interphases, and 95~99% of chorionic villus cell interphases showed expected signals. One suspected female was identified as deletion carriers and two were excluded.Conclusion FISH in combination with other available techniques allows efficient screening of DMD/BMD deletion carriers, which also lay the ground work for prenatal diagnosis for potential fetal carriers.

  10. Regulation of Pattern Formation and Gene Amplification During Drosophila Oogenesis by the miR-318 microRNA

    DEFF Research Database (Denmark)

    Ge, Wanzhong; Deng, Qiannan; Guo, Ting;

    2015-01-01

    Pattern formation during epithelial development requires the coordination of multiple signaling pathways. Here, we investigate the functions of an ovary-enriched miRNA, miR-318, in epithelial development during Drosophila oogenesis. miR-318 maternal loss-of-function mutants were female sterile...... and laid eggs with abnormal morphology. Removal of miR-318 disrupted the dorsal-anterior follicle cell patterning, resulting in abnormal dorsal appendages. miR-318 mutant females also produced thin and fragile eggshells, due to impaired chorion gene amplification. We provide evidence that the ecdysone...... signaling pathway activates expression of miR-318 and that miR-318 cooperates with Tramtrack69 (Ttk69) to control the switch from endocycling to chorion gene amplification during differentiation of the follicular epithelium. The multiple functions of miR-318 in oogenesis illustrate the importance of mi...

  11. Ootaxonomic investigation of five Lutzomyia species (Diptera, Psychodidae from Venezuela

    Directory of Open Access Journals (Sweden)

    AM Fausto

    2001-02-01

    Full Text Available The eggshell fine structure of five sand fly species from Venezuela belonging to the genus Lutzomyia (L. migonei, L. ovallesi, L. absonodonta, L. gomezi and L. panamensis was examined by scanning electron microscopy. The chorionic sculpturing of L. migonei, L. ovallesi, L. absonodonta and L. gomezi was characterized by series of columns arranged in palisade to form sinuous ridges. In inter-ridge areas, the basal layer was covered with fibrous material. The outer chorion of L. panamensis had a pattern known as "mountain- or volcano-like". The morphology of the posterior pole and aeropyle had a common structure in the five species, with some species-specific characters. The eggshell features of the five species are compared with those of other phlebotomine sand flies.

  12. Variation of urinary reproductive hormones of a captive female African elephant during gestation%圈养雌性非洲象妊娠期尿中性激素水平的变化

    Institute of Scientific and Technical Information of China (English)

    刘学锋; 张耀华; 秦志刚; 王璐; 原蕾; 张轶卓

    2012-01-01

    In this study we monitored the urinary levels of progesterone (P), estradiol (E2) , estriol (E3) and chorion-ic gonadotropin (CG) of a female African elephant (Loxodonta africana) during pregnancy using radioimmunoassay. It was shown that progesterone levels increased gradually during early pregnancy, peaking at the 4th - 6th month of pregnancy and then dropping to the basal level at delivery. The estriol concentration decreased slowly during gestation, whereas the estradiol level did not change significantly during pregnancy. This is the first report of CG during gestation of African elephant. CG level was almost undetectable in the first three month of pregnancy, and it began to rise slowly after the progesterone peak (4-6 month of gestation). These results provide references for changes of P and CG in pregnancy.

  13. Alcohol consumption at the time of conception and spontaneous abortion

    DEFF Research Database (Denmark)

    Henriksen, Tine Brink; Hjollund, Niels Henrik; Jensen, Tina Kold;

    2004-01-01

    The authors studied the association between female and male alcohol intakes at the time of conception and the risk of spontaneous abortion, including early pregnancy loss detected by urinary human chorionic gonadotropin. After a nationwide mailing to about 50,000 members of four trade unions....... Women collected morning urine for 10 days from the first day of vaginal bleeding in each cycle. The authors detected 186 pregnancies: 131 resulted in childbirth, and 55 resulted in spontaneous abortion (34 detected by urinary human chorionic gonadotropin). Depending on the intake in the cycle...... of conception and the adjustment factors, female alcohol intake was associated with 2-3 times the adjusted risk of spontaneous abortion compared with no intake, and male alcohol intake was associated with 2-5 times the adjusted risk. Only the adjusted relative risks for 10 or more drinks/week compared...

  14. First-line chemotherapy in low-risk gestational trophoblastic neoplasia.

    LENUS (Irish Health Repository)

    Alazzam, Mo'iad

    2012-01-01

    This is an update of a Cochrane review that was first published in Issue 1, 2009. Gestational trophoblastic neoplasia (GTN) is a rare but curable disease arising in the fetal chorion during pregnancy. Most women with low-risk GTN will be cured by evacuation of the uterus with or without single-agent chemotherapy. However, chemotherapy regimens vary between treatment centres worldwide and the comparable benefits and risks of these different regimens are unclear.

  15. Frequencies of fetal chromosomal abnormalities at prenatal diagnosis: 10 years experiences in a single institution.

    OpenAIRE

    Park, S. Y.; J.W. Kim; Y.M. Kim; Kim, J.M.; Lee, M. H.; Lee, B. Y.; Han, J. Y.; Kim, M. Y.; Yang, J. H.; Ryu, H. M.

    2001-01-01

    We present frequencies of fetal chromosomal abnormalities in 4,907 prenatal cytogenetic examinations at Samsung Cheil Hospital from 1988 to 1997 for 10 yr duration. Prenatal karyotypes were undertaken in 3,913 amniotic fluid samples, 800 chorionic villi samples, and 194 percutaneous umbilical blood samples. The frequency of fetal abnormal karyotypes was 3.1% (150 cases). Numerical chromosome abnormalities were 87 cases (1.8%) and structural aberrations of chromosomes were 63 cases (1.3%). In ...

  16. First-Trimester Combined Screening Is Effective for the Detection of Unbalanced Chromosomal Translocations at 11 to 12 Weeks of Gestation

    OpenAIRE

    Huang, ShangYu; Chang, Chialin; Cheng, PoJen; Hsiao, ChingHua; Soong, YungKuei; Duan, Tao

    2014-01-01

    The first trimester combined screening, which analyzes fetal nuchal translucency and levels of free β-human chorionic gonadotropin (β-hCG) and pregnancy-associated plasma protein A (PAPP-A) in maternal serum, is routinely used to detect abnormal pregnancies associated with Down syndrome and other trisomy aneuploidies. Based on the hypothesis that major chromosomal translocations could lead to similar biochemical and developmental outcomes during early embryo development, we compared these mar...

  17. The Incidence and Type of Chromosomal Translocations from Prenatal Diagnosis of 3800 Patients in the Republic of Macedonia

    OpenAIRE

    Vasilevska, M; Ivanovska, E; Kubelka Sabit, K; E. Sukarova-Angelovska; Dimeska, G

    2013-01-01

    Robertsonian and reciprocal chromosomal translocations are the most frequent type of structural chromosomal aberrations in the human population. We report the frequency and type of detected translocations in 10 years of prenatal diagnosis of 3800 prenatal samples. The materials came from amniocentesis and chorionic villus samples (CVS). We detected seven Robertsonian translocations (0.18%), eight autosomal reciprocal translocations (0.21%) and one sex chromosome translocation (0.03%). The ove...

  18. Sviluppo di una piattaforma per la diagnosi prenatale non invasiva di malattie genetiche in epoca gestazionale precoce

    OpenAIRE

    Capponi, Valentina

    2016-01-01

    Prenatal diagnosis of aneuploidies and monogenic diseases is usually performed by amniocentesis or chorionic villous sampling. However, these procedures are associated with 0.5%-2% risk of miscarriage. The discovery of cell free fetal DNA (cffDNA) in maternal plasma in 1997 has provided a new source of fetal genetic material that can be safely obtained from maternal blood and successfully processed for non invasive genetic diagnosis (NIPD). In this study is described a new a...

  19. Serum screening in first trimester to predict pre-eclampsia, small for gestational age and preterm delivery: systematic review and meta-analysis

    OpenAIRE

    Zhong, Yan; Zhu, Fufan; Ding, Yiling

    2015-01-01

    Background Early assessment before the establishment of placental dysfunction has the potential to improve treatment and prognosis for clinical practice.The objective of the study is to investigate the accuracy of serum biochemical markers(Pregnancy- Associated Plasma Protein-A (PAPP-A), human Chorionic Gonadotropin (hCG), Placental Growth Factor (PlGF), Placental Protein 13 (PP13) used in first trimester serum screening in predicting preelampsia, small for gestational age (SGA) and preterm d...

  20. Subclinical abortions in patients treated with clomiphene citrate

    International Nuclear Information System (INIS)

    Using radioimmunoassay for human chorionic gonadotrophin beta-subunit, 39 treatment cycles of clomiphene citrate therapy were studied prospectively for incidence of subclinical abortions. Eight treatment cycles resulted in clinically recognizable pregnancies and three other treatment cycles ended up with subclinical abortions. The plasma progesterone levels in patients with subclinical abortions at the 13th day after ovulation were lower than those in patients with normal pregnancies. (author)

  1. Breast carcinoma with choriocarcinomatous features: a case report and review of the literature

    OpenAIRE

    Zhu, Yanyun; Liu, Mei; Li, Jinyu; Jing, Fangfang; Linghu, Ruixia; Guo, Xiaoqin; JIAO, SHUNCHANG; Yang, Junlan

    2014-01-01

    Background Breast carcinoma with choriocarcinomatous features (BCCF) is a rare variant of breast cancer, characterized by high expression of human chorionic gonadotropin (HCG) in cancer cells such as multinucleated syncytiotrophoblast-like giant cells. The first case of BCCF was reported in 1981 by Saigo and Rosen. Only one case of BCCF was reported to show no component of breast ductal carcinoma, and only partially cancer cells, such as multinucleated syncytiotrophoblast-like giant cells, ex...

  2. Advances in the management of metastatic non-seminomatous germ cell tumours during the cisplatin era: a single-institution experience.

    OpenAIRE

    Gerl, A; Clemm, C.; Schmeller, N.; Hartenstein, R.; Lamerz, R.; Wilmanns, W.

    1996-01-01

    Long-term outcome was reviewed in 266 consecutive patients with metastatic non-seminomatous germ cell tumours treated at a single institution. The overall 3 year survival was 77%, and 3 year progression-free survival was 71%. Multivariate analysis identified the following clinical features as independent prognostic factors: the presence of liver, bone or brain metastasis, serum human chorionic gonadotropin > or = 10000 U l-1 and/or alpha-fetoprotein > or = 1000 ng ml-1, a mediastinal mass > 5...

  3. The efficacy of recombinant versus urinary HCG in ART outcome

    OpenAIRE

    Maryam Eftekhar; Mohammad Ali Khalili; Elham Rahmani

    2012-01-01

    Background: Human chorionic gonadotropin (HCG) has been used as a replacement for the mid-cycle luteinizing hormone (LH) surge for several years. The recent arrival of recombinant DNA technology has made recombinant HCG (rHCG) accessible. Objective: To assess efficacy of rHCG compared to urinary HCG (uHCG) for triggering of ovulation and induction of final oocyte maturation in assisted reproductive cycles. Materials and Methods: 200 patients who were candidate for ICSI were randomly divided i...

  4. Prenatal diagnosis of morquio disease type A using a simple fluorometric enzyme assay

    OpenAIRE

    Zhao, Hui; Diggelen, Otto; Thoomes, R.; Huijmans, Jan; Young, E.; Mazurczak, T; Kleijer, Wim

    1990-01-01

    textabstractA new fluorogenic substrate, 4 methylumbelliferyl B-D-6-sulphogalactoside, was used for the assay of galactose-6-sulphate sulphatase activity in chorionic villi, cultured villus cells, and amniocytes. The fluorometric assay is much more convenient than the conventional assay using radiolabelled, sulphated oligosaccharides. Both types of substrate were used in the prenatal diagnosis of three pregnancies at risk for Morquio type A disease using amniocytes. These enzyme tests, as wel...

  5. Human pituitary and placental hormones control human insulin-like growth factor II secretion in human granulosa cells.

    OpenAIRE

    Ramasharma, K; Li, C. H.

    1987-01-01

    Human granulosa cells cultured with calf serum actively proliferated for 18-20 generation and secreted progesterone into the medium; progesterone levels appeared to decline with increase in generation number. Cells cultured under serum-free conditions secreted significant amounts of progesterone and insulin-like growth factor II (IGF-II). The progesterone secretion was enhanced by the addition of human follitropin, lutropin, and chorionic gonadotropin but not by growth hormone. These cells, w...

  6. Genetic analysis of familial isolated growth hormone deficiency type I.

    OpenAIRE

    Phillips, J. A.; Parks, J. S.; Hjelle, B L; Herd, J E; Plotnick, L P; Migeon, C. J.; Seeburg, P H

    1982-01-01

    Nuclear DNA from individuals belonging to nine different families in which two sibs were affected with isolated growth hormone deficiency type I were studied by restriction endonuclease analysis. By using 32P-labeled human growth hormone or the homologous human chorionic somatomammotropin complementary DNA (cDNA) sequences as a probe, the growth hormone genes of affected individuals from all families yielded normal restriction patterns. Polymorphic restriction endonuclease sites (HincII and M...

  7. Adnexal mass in tubal pregnancy

    International Nuclear Information System (INIS)

    Off the evaluation of clinical significance and pathologic correlation of echogenic adnexal mass in tubal pregnancy, which is separated from ipsilateral ovary, we performed a retrospective analysis of 15 proved tubal pregnancies. All showed hematosalpinx containing blood coagulum and chorionic villi with intact fallopian tube.The echogenicity of the adnexal mass was considered to represent the nonclotted or clotted blood within the fallopian tube. We conclude that the discrete, echogenic adnexal mass is the suggestive finding of unrupturedtubal pregnancy

  8. Synthesis of fucosyl-containing glycoproteins of the vitelline coat in oocytes of Ciona intestinalis (Ascidia).

    OpenAIRE

    F.Rosati; Cotelli, F.; De Santis, R.; A. Monroy; Pinto, M. R.

    1982-01-01

    The sperm receptors of the ascidian oocyte are located at the outer surface of the vitelline coat (formerly called the chorion). The fucose residues are the receptor's most important components for sperm recognition and binding. We asked whether the fucosyl-containing glycoproteins of the vitelline coat are a product of the oocyte, the follicle cells, or the test cells. Ovaries of Ciona intestinalis were injected with L-[3H]fucose and the progress of its incorporation was followed by using au...

  9. Injectable polymer microspheres enhance immunogenicity of a contraceptive peptide vaccine

    OpenAIRE

    Cui, Chengji; Stevens, Vernon C.; Schwendeman, Steven P.

    2006-01-01

    Advanced contraceptive peptide vaccines suffer from the unavailability of adjuvants capable of enhancing the antibody response with acceptable safety. We sought to overcome this limitation by employing two novel poly(lactic-co-glycolic acid) (PLGA) microsphere formulations to deliver a synthetic human chorionic gonadotropin (hCG) peptide antigen co-synthesized with a T-cell epitope from tetanus toxoid, C-TT2-CTP35: surface-conjugated immunogen to induce phagocytosis; and encapsulated peptide ...

  10. In-vitro maturation and cryopreservation of oocytes at the time of oophorectomy

    OpenAIRE

    Walls, Melanie L.; Kirsty Douglas; Ryan, John P.; Jason Tan; Roger Hart

    2015-01-01

    A 27 year old female presented for fertility preservation prior to undergoing pelvic radiotherapy. She had previously undergone a radical laparoscopic hysterectomy for cervical carcinoma seven months earlier. A trans-vaginal oocyte aspiration was not advisable due to a vaginal recurrence of the disease. Due to a polycystic ovarian morphology (PCO), follicle stimulating hormone (FSH) priming with no human chorionic gonadotrophin (hCG) trigger was performed prior to oophorectomy followed by ex-...

  11. Etude de nouveaux biomarqueurs de toxicité induite par des micropolluants (benzo(a)pyrène et phtalate de bis(2-ethylhexyle)) sur des modèles de placenta humain

    OpenAIRE

    Wakx, Anaïs,

    2014-01-01

    Prenatal exposure to pollutants is commonly evaluated using placenta as a barrier between mother and fetus. Here, we consider placenta as a target organ for toxic agents. To achieve this, we selected a trophoblastic cell model, which is adapted to toxicological studies. In clinical studies, pregnancy pathologies are associated to changes in human placental lactogen (hPL) and human chorionic gonadotropin (hCG) secretions. Our in vitro work links exposure to micropollutants (mono(2-ethylhexyl)p...

  12. First-trimester prenatal diagnosis of Tay-Sachs disease.

    OpenAIRE

    Grabowski, G. A.; Kruse, J. R.; Goldberg, J D; Chockkalingam, K; Gordon, R E; Blakemore, K J; Mahoney, M. J.; Desnick, R J

    1984-01-01

    The prenatal diagnosis of Tay-Sachs disease was made in two at-risk fetuses by the analysis of chorionic villi obtained at 9 and 11 menstrual weeks, respectively. The diagnoses were based on the absence of beta-hexosaminidase A activity as determined by: (1) specific enzyme assays, (2) anion-exchange chromatography, and (3) cellulose acetate gel electrophoresis. The enzymatic diagnoses were confirmed on fetal tissue as well as by ultrastructural demonstration of the first-trimester fetal neur...

  13. Molar Pregnancy with False Negative β-hCG Urine in the Emergency Department

    OpenAIRE

    Hunter, Christopher L.; Ladde, Jay

    2011-01-01

    This case describes an atypical presentation of molar pregnancy in an emergency department patient with abdominal pain and vaginal bleeding. The patient demonstrated clinical features of hydatidiform mole, including acute discharge of a large, grape-like vesicular mass, despite multiple negative urine pregnancy tests. These false-negative qualitative human chorionic gonadotropin assays were likely caused by the “high-dose hook effect” and may have delayed proper care of the patient, who displ...

  14. Embolization of uterine artery as an emergency treatment for vaginal bleeding due to ectopic cervical pregnancy

    OpenAIRE

    Hari A; Indira B; Venkateswarlu J

    2015-01-01

    A 25-year-old, primigravida presented with a history of vaginal bleeding since 3 days. Two months ago she had conceived spontaneously. Pelvic ultrasonography revealed cervical pregnancy of 8 weeks duration. Parenteral methotrexate failed to terminate pregnancy and serum beta- human chorionic gonadotropin levels continued to rise. In order to achieve haemostasis, catheter angiography and bilateral uterine artery embolization were done followed by curettage of cervical canal. The patient recove...

  15. Non-invasive prenatal diagnosis of fetal trisomy 21 using cell-free fetal DNA in maternal blood

    OpenAIRE

    Lim, Ji Hyae; Park, So Yeon; Ryu, Hyun Mee

    2013-01-01

    Since the existence of cell-free fetal DNA (cff-DNA) in maternal circulation was discovered, it has been identified as a promising source of fetal genetic material in the development of reliable methods for non-invasive prenatal diagnosis (NIPD) of fetal trisomy 21 (T21). Currently, a prenatal diagnosis of fetal T21 is achieved through invasive techniques, such as chorionic villus sampling or amniocentesis. However, such invasive diagnostic tests are expensive, require expert technicians, and...

  16. Noninvasive prenatal diagnosis of fetal RhD status using cell-free fetal DNA in maternal plasma with TaqMan® real-time PCR assay

    OpenAIRE

    Rekhviashvili, Tea

    2007-01-01

    Prenatal diagnosis is now part of established obstetric practice in many countries. However, conventional methods of prenatal diagnosis of obtaining fetal tissues for genetic analysis, including amniocentesis and chorionic villus sampling, are invasive and constitute a finite risk to the unborn fetus1. At present, it is widely accepted that both intact fetal cells as well as cell-free fetal DN A are present in the maternal circulation and can be recovered for non-invasive prena...

  17. Prenatal diagnosis of hemoglobinopathies: from fetoscopy to coelocentesis

    OpenAIRE

    Gianfranca Damiani; Margherita Vinciguerra; Cristina Jakil; Monica Cannata; Filippo Cassarà; Francesco Picciotto; Giovanna Schillaci; Valentina Cigna; Disma Renda; Aldo Volpes; Francesca Sammartano; Samuela Milone; Adolfo Allegra; Cristina Passarello; Filippo Leto

    2014-01-01

    Prenatal diagnosis of hemoglobinopathies involves the study of fetal material from blood, amniocytes, trophoblast coelomatic cells and fetal DNA in maternal circulation. Its first application dates back to the 70s and it involves globin chain synthesis analysis on fetal blood. In the 1980s molecular analysis was introduced as well as amniocentesis and chorionic villi sampling under high-resolution ultrasound imaging. The application of direct sequencing and polymerase chain reactionbased meth...

  18. Tracking fetal development through molecular analysis of maternal biofluids☆

    OpenAIRE

    Edlow, Andrea G; Bianchi, Diana W.

    2012-01-01

    Current monitoring of fetal development includes fetal ultrasonography, chorionic villus sampling or amniocentesis for chromosome analysis, and maternal serum biochemical screening for analytes associated with aneuploidy and open neural tube defects. Over the last 15 years, significant advances in noninvasive prenatal diagnosis (NIPD) via cell-free fetal (cff) nucleic acids in maternal plasma have resulted in the ability to determine fetal sex, RhD genotype, and aneuploidy. Cff nucleic acids ...

  19. Simple method for backyard production of snakehead (Channa striata Bloch) fry

    OpenAIRE

    Yaakov, W.A.A.W.; Ali, A. B.

    1992-01-01

    The snakehead (Channa striata ) is a common freshwater fish species in Malaysia. Details are given of a simple technique for breeding this species, suitable for small-scale farmers practising backyard aquaculture. Two techniques were used to induce spawning - the first used water level manipulation to simulate rain and the second used injected with human chorionic gonadotropic hormone. The former, more natural, spawning technique was found to provide a viable alternative for the small-scale f...

  20. Relationship between the serum B-HCG and preeclempsia and itsseverity

    OpenAIRE

    Z Basirat; Sh. Barat; M.Haji Ahmadi

    2006-01-01

    Background and purpose: Preeclampsia as the most common complication of pregnancies has unknown physiopathology. Because the role of serum Beta-human Chorionic Gonadotropin (B-hCG) in pathophysiology of preeclampsia is not well known, we decided to determine the correlation between serum concentration of B-HCG and preeclampsia .Materials and Methods: This case-control study performed on two groups of 40 preeclamptic nulliparous and 40 healthy term pregnant women at obstetrics and gynecology w...

  1. Choriocarcinoma presenting with thyrotoxicosis

    OpenAIRE

    Sotello, David; Rivas, Ana Marcella; Test, Victor J.; Lado-Abeal, Joaquin

    2016-01-01

    We describe a 26-year-old man with metastatic choriocarcinoma who presented with hyperthyroidism associated with elevated β-human chorionic gonadotropin (B-HCG) and respiratory failure secondary to diffuse lung metastasis. After the first cycle of chemotherapy, the concentration of B-HCG dramatically decreased and the patient became euthyroid, allowing us to discontinue antithyroid medications. The patient's hyperthyroidism was caused by stimulation of the thyroid gland by high B-HCG levels, ...

  2. Perbaikan Fenotipe Pertumbuhan Anak Babi Lokal Melalui Penyuntikan Gonadotropin Sebelum Induk Dikawinkan (IMPROVEMENT OF GROWTH PHENOTYPE OF LOCAL PIGLET BY GONADOTROPHIN INJECTION OF SOW PRIOR TO MATING)

    OpenAIRE

    1Debby Jacqueline Jochebed Rayer; Muladno; Hera Maheshwari; Wasmen Manalu

    2016-01-01

    An experiment was designed to study the growth phenotypes of piglets born to sows injected withpregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) prior to mating inorder to improve endogenous secretions of pregnant hormones during pregnancy. The experimental sowsused in this study were 10 local breed sows with body weight ranges of 30-40 kg. Before mating, estrouscycles of the experimental sows were synchronized by injecting 3.75 mg prostaglandin twice with 14 days...

  3. The Camel Tick, Hyalomma (Hyalomma dromedarii Koch, 1844 (Ixodoidea: Ixodidae: Description of the Egg and Redescription of the Larva by Scanning Electron Microscopy

    Directory of Open Access Journals (Sweden)

    Ashraf Ahmed Montasser

    2006-01-01

    Full Text Available The present study describes the egg of Hyalomma (H. dromedarii for the first time and adds more features to the larva using Scanning Electron Microscopy (SEM in order to extend our knowledge on these acarine stages to be useful for further taxonomical or control studies. With the purpose of presenting exact description of acarine eggs, it is preferable to examine them both with and without SEM processing. SEM processing caused partial removal of the chorion which makes the egg shell clear and easily observed. The study revealed rough surface of egg shell which was surrounded by the chorion. The egg shell was perforated particularly at poles. The chorion appeared as a finely perforated cloth. Different forms of bumps were noticed between egg shell and chorion. Length, width, l/w ratio and pore diameter of the egg were measured. SEM investigation of the larva revealed smooth scutum with slight irregular ornamentation and horizontally folded extensible cuticle with vertical ridges. At least 2 types of cuticular openings were noticed on the extensible cuticle of the idiosoma. The first type was represented by 1 pair on dorsal side and 2 pairs on ventral one. It was surrounded with thick integumental ring and guarded with 2 internal lips. The second type was numerous, slit-like and without rings or lips. Dorsolateral plate of the hypostome carried numerous oval, tile-like and elevated denticles while ventral one carried 4 rows of posteriorly directed retrograde conical denticles. Mouth enclosed 2 cheliceral digits, each terminated with 3 lobes. Each lobe is supported with 2 or 3 conical denticles which were externally directed to the posterior. Haller`s organ on the tarsus of the first pair of legs consisted of anterior pit and posterior capsule. The pit contained 6 conical sensillae while the capsule opening had extensively branched margin. Measurements of the whole body, idiosoma, scutum, eye, capitulum, hypostome, palp, cuticular pores, legs and

  4. Risk assessment adjusted for gestational age in maternal serum screening for Down's syndrome.

    OpenAIRE

    Gardosi, J.; Mongelli, M.

    1993-01-01

    OBJECTIVE--To investigate the relation between errors in calculation of gestational age and assessment of risk of Down's syndrome and to analyse the implications for screening programmes. DESIGN--Retrospective analysis of dating of gestational age by menstrual history v ultrasound scan. Computer program with maternal age and concentrations of alpha fetoprotein and free beta human chorionic gonadotrophin to calculate risk for a range of expected dates of delivery. Computer simulated prospectiv...

  5. Non-Invasive Prenatal Testing

    OpenAIRE

    McGillivray, Barbara C.

    1988-01-01

    The rate of newborns with trisomy 21 (Down syndrome) who have been referred to our pediatric newborn clinic is very high. This shows that prenatal screening in the region is not carried out well. Prenatal diagnosis and screening methods include invasive prenatal diagnosis methods (amniocentesis, chorionic villus sampling (CVS), and cordocentesis) and non-invasive prenatal diagnosis (NIPT) which cell free fetal DNA (cffDNA) screening of maternal blood samples. After the discovery of the signs ...

  6. Messenger Ribonucleic Acid Expressions of Hepatocyte Growth Factor, Angiopoietins and Their Receptors During Follicular Development in Gilts

    OpenAIRE

    Shimizu, Takashi; Iijima, Koji; SASADA, Hiroshi; Sato, Eimei; 清水, 隆

    2003-01-01

    Angiogenic factors are associated with angiogenesis during follicular development in the mammalian ovary. The aim of the present study was to determine the relationships between the vascular network and mRNA expressions of angiopoietins (Ang)-1, Ang-2 and hepatocyte growth factor (HGF), and their receptors in follicles at different developmental stages during follicular development. Ovaries in gilts were collected 72 h after equine chorionic gonadotropin (eCG, 1250 IU) treatment for histologi...

  7. Strategy for reliable prenatal detection of normal male carriers of the fragile X syndrome

    OpenAIRE

    Halley, Dicky; Ouweland, Ans; Deelen, Wouter; Verma, Chandra; Oostra, Ben

    1994-01-01

    textabstractPrenatal diagnosis of fragile X syndrome identifying full mutations has been described. Here we report on a case of a prenatal test concerning a normal male carrier of the fragile X syndrome. Southern blot analysis of the fragile X gene resulted in the identification of a premutation in DNA isolated from the chorionic villus sample. Using a polymerase chain reaction (PCR)based assay the CGG repeat length was determined to be 82 CGG repeat units. Confirmation of this premutation in...

  8. Coexistence of Gastric Adenocarcinoma and Choriocarcinoma: Complete Response to Trastuzumab and Chemotherapy

    OpenAIRE

    GUNDUZ, SEYDA; Elpek, Gulsum Ozlem; Uysal, Mukremin; Goksu, Sema Sezgin; Tatli, Murat; Arslan, Deniz; Coskun, Hasan Senol; BOZCUK, Hakan; Savas, Burhan; Ozdogan, Mustafa

    2012-01-01

    Gastric choriocarcinoma is a rare neoplasm and usually accompanies gastric adenocarcinoma. The prognosis is poor due to the aggressive course of the disease. A 57-year-old female patient with weight loss and abdominal pain was examined. The patient was operated following the examination, and pathological analysis revealed the presence of a gastric adenocarcinoma associated with choriocarcinoma. Immunohistochemical analysis showed a positive reaction with antibodies to beta-human chorionic gon...

  9. Circulating Cell Free DNA in the Diagnosis of Trophoblastic Tumors

    OpenAIRE

    Openshaw, Mark R.; Harvey, Richard A.; Sebire, Neil J; Baljeet Kaur; Naveed Sarwar; Michael J Seckl; Fisher, Rosemary A.

    2015-01-01

    Gestational trophoblastic neoplasia (GTN) represents a group of diseases characterized by production of human chorionic gonadotropin (hCG). Since non-gestational tumors may occasionally secrete hCG, histopathological diagnosis is important for appropriate clinical management. However, a histopathological diagnosis is not always available. We therefore investigated the feasibility of extracting cell free DNA (cfDNA) from the plasma of women with GTN for use as a “liquid biopsy” in patients wit...

  10. Heptageniidae (Insecta, Ephemeroptera of Thailand

    Directory of Open Access Journals (Sweden)

    Boonsatien Boonsoong

    2013-02-01

    Full Text Available Nine genera and twenty-two species of heptageniid mayflies from Thailand are defined in this present work as well as one suggested further subgenus, Compsoneuria (Siamoneuria kovaci (species “incertae sedis” including some particular characters. Taxonomic remarks, diagnoses, line drawings of key characters, distribution, habitat and biological data, and a larval key to the genera and species are provided. The chorionic eggs of eight genera and eight species were observed and shown using a scanning electron microscope.

  11. Clinical Variables Affecting The Pregnancy Rate of Intracervical Insemination Using Cryopreserved Donor Spermatozoa: A Retrospective Study in China

    OpenAIRE

    Xiao-Jun Chen; Li-Ping Wu; Hai-Lian Lan; Li Zhang; Yi-Min Zhu

    2012-01-01

    Background: The aim of this study was to investigate whether several clinical variables can affect the pregnancy rate of intracervical insemination (ICI) using cryopreserved donor spermatozoa. Materials and Methods: In this retrospective study, age, years of infertility, cervicitis, urinary luteinizing hormone (LH) surge, insemination number, uterus position, endometrial thickness and morphology, maximal follicle diameter, and the number of dominant follicles on the day of human chorionic gon...

  12. Luteal phase support for assisted reproduction cycles (Review)

    OpenAIRE

    Linden, M.; Buckingham, K.; Farquhar, C; Kremer, J A M; Metwally, M.

    2011-01-01

    BACKGROUND: Progesterone prepares the endometrium for pregnancy by stimulating proliferation in response to human chorionic gonadotropin (hCG), which is produced by the corpus luteum. This occurs in the luteal phase of the menstrual cycle. In assisted reproduction techniques (ART) the progesterone or hCG levels, or both, are low and the natural process is insufficient, so the luteal phase is supported with either progesterone, hCG or gonadotropin releasing hormone (GnRH) agonists. Luteal phas...

  13. Role of human cytomegalovirus in the proliferation and invasion of extravillous cytotrophoblasts isolated from early placentae

    OpenAIRE

    Liu, Tao; Zheng, Xiaofei; Li, Qin; Chen, Juanjuan; Yin, Zongzhi; Xiao, Juan; Zhang, Dandan; Li, Wei; Qiao, Yuan; Chen, Suhua

    2015-01-01

    Aim: We investigated the role of human cytomegalovirus (HCMV) and its mechanism in extravillous cytotrophoblast (EVT) proliferation and invasion in vitro. Methods: Differential enzymatic digestion combined with gradient centrifugation, was used to isolate primary EVT from human chorionic villi collected from early placentae of healthy pregnant women. HCMV infection was determined by immunofluorescence staining of HCMVpp65 antigen expression. An MTT assay was used to examine the role of HCMV i...

  14. 46,XY,DUP(10Q) IN DIRECT CVS PREPARATION AND MOSAIC 48,XXXY,DUP(10Q) IN CVS LONG-TERM CULTURE AND FETAL TISSUE

    NARCIS (Netherlands)

    SIJMONS, RH; SIKKEMARADDATZ, B; KLOOSTERMAN, MD; BRIET, JW; DEJONG, B; LESCHOT, NJ

    1995-01-01

    Chorionic villus sampling (CVS) was performed on a 40-year-old woman at 9 1/2 menstrual weeks because of advanced maternal age. The direct preparation showed 46,XY,dup(10)(q11.2q23.2). CVS long-term culture and fetal tissue revealed a rare additional abnormality: 48,XXXY,dup(10)(q11.2q23.2). This ab

  15. Short-Term Pharmacological Suppression of the Hyperprolactinemia of Infertile hCG-Overproducing Female Mice Persistently Restores Their Fertility

    OpenAIRE

    Ratner, Laura D.; Gonzalez, Betina; Ahtiainen, Petteri; Di Giorgio, Noelia P.; Poutanen, Matti; Calandra, Ricardo S.; Ilpo T Huhtaniemi; Rulli, Susana B.

    2012-01-01

    Female infertility is often associated with deregulation of hormonal networks, and hyperprolactinemia is one of the most common endocrine disorders of the hypothalamic-pituitary axis affecting the reproductive functions. We have shown previously that transgenic female mice overexpressing human chorionic gonadotropin β-subunit (hCGβ+ mice), and producing elevated levels of bioactive LH/hCG, exhibit increased production of testosterone and progesterone, are overweight and infertile, and develop...

  16. Цитогенетичний аналіз хоріону при вагітності, що не розвивається, за матеріалами регіонального репродуктивного центру

    OpenAIRE

    Avramenko, N. V.; Barkovsky, D. Ye.; Semenenko, I. V.; Kalabuchova, N. A.

    2015-01-01

    Aim. Detection of chromosomal aberrations in patients during spontaneous abortion.Methods and results. Cytogenetic study of chorionic samples of the abortions on first trimester of pregnancy. According to the study, the karyotype anomalies were the cause of cardiac embryonic development in 59% of cases. Conclusion. Due to numerous cytogenetic researches of abortion material, anomalies of karyotype are the leading reason of embryonic destruction. The present paper summarizes results of cytogen...

  17. EST Table: BY927519 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available e binding)|GO:0050826(response to freezing) 10/09/28 53 %/117 aa prf||1209265U chorion protein B11 10/08/29 n.h 10/08/28 n.h 10/09/10 n.h 10/09/10 n.h 10/09/10 n.h BY927519 fcP8 ...

  18. Environmental Levels of para-Nonylphenol Are Able to Affect Cytokine Secretion in Human Placenta

    OpenAIRE

    Bechi, Nicoletta; Ietta, Francesca; Romagnoli, Roberta; Jantra, Silke; Cencini, Marco; Galassi, Gianmichele; Serchi, Tommaso; Corsi, Ilaria; Focardi, Silvano; Paulesu, Luana

    2009-01-01

    Background para-Nonylphenol (p-NP) is a metabolite of alkylphenols widely used in the chemical industry and manufacturing. It accumulates in the environment, where it acts with estrogen-like activity. We previously showed that p-NP acts on human placenta by inducing trophoblast differentiation and apoptosis. Objective The aim of the present study was to investigate the effect of p-NP on cytokine secretion in human placenta. Methods In vitro cultures of chorionic villous explants from human pl...

  19. Macrophage Migration Inhibitory Factor Is Up-Regulated in Human First-Trimester Placenta Stimulated by Soluble Antigen of Toxoplasma gondii, Resulting in Increased Monocyte Adhesion on Villous Explants

    OpenAIRE

    Ferro, Eloisa Amália Vieira; Mineo, José Roberto; Ietta, Francesca; Bechi, Nicoletta; Romagnoli, Roberta; Silva, Deise Aparecida Oliveira; Sorda, Giuseppina; Bevilacqua, Estela; Paulesu, Luana Ricci

    2008-01-01

    Considering the potential role of macrophage migration inhibitory factor (MIF) in the inflammation process in placenta when infected by pathogens, we investigated the production of this cytokine in chorionic villous explants obtained from human first-trimester placentas stimulated with soluble antigen from Toxoplasma gondii (STAg). Parallel cultures were performed with villous explants stimulated with STAg, interferon-γ (IFN-γ), or STAg plus IFN-γ. To assess the role of placental MIF on monoc...

  20. Plasmodium falciparum induces a Th1/Th2 disequilibrium, favoring the Th1-type pathway, in the human placenta

    OpenAIRE

    Fievet, Nadine; Moussa, M.; Tami, G.; Maubert, B.; Cot, Michel; Deloron, P.; Chaouat, G.

    2001-01-01

    During pregnancy, a local and systemic Th2 bias of maternal immunity favors Th1-dependent infections such as malaria. This study measured cytokines secreted in cultures of chorionic villi, placental blood cells (PBC), and serum in term placentas from 88 malaria-infected and -noninfected Cameroon women. Interleukin (IL)-2 and-4 were consistently low ; IL-1Beta, IL-6, granulocyte-macrophage colony-stimulating factor, and transforming growth factor (TGF)-Beta2 were highest in PBC cultures. Malar...

  1. Left-sided gastroschisis with placenta findings: case report and literature review

    OpenAIRE

    Shi, Yang; Farinelli, Christine K; Chang, Michael S; CARPENTER, PHILIP M.

    2012-01-01

    Gastroschisis is a congenital abdominal-wall defect that typically occurs to the right of the umbilicus. Only twenty-one cases of left-sided gastroschisis have been described in the literature. Here we report a large left-sided gastroschisis with pulmonary hypoplasia, scoliosis, ventricular septal defect and absence of gallbladder. Section of placental membranes revealed vacuolization of the amnion, without increased macrophage infiltration of the chorion. Postmortem comparative genomic hybri...

  2. Morphological and Physiological Characteristics that Contribute to Insecticide Resistance in Bed Bug (Cimex lectularius L.) Eggs.

    OpenAIRE

    Delong, Brittany E

    2014-01-01

    Although bed bug eggs are a difficult life stage to control with our currently labeled insecticides, few studies have examined how bed bug egg morphology and physiology is potentially related to pesticide resistance in bed bug eggs. Bed bug egg morphological features were examined using scanning electron microscopy (SEM) and the chorion and respiration structures were identified. Scanning electron microscopy photographs and bed bug egg measurements indicated there were no morphological differ...

  3. Maternal use of acetaminophen, ibuprofen, and acetylsalicylic acid during pregnancy and risk of cryptorchidism

    DEFF Research Database (Denmark)

    Jensen, Morten Søndergaard; Rebordosa, Cristina; Thulstrup, Ane Marie;

    2010-01-01

    Cyclooxygenase (COX) inhibitors-acetaminophen, ibuprofen and acetylsalicylic acid-have endocrine-disruptive properties in the rainbow trout. In humans, aspirin blocks the androgen response to human chorionic gonadotropin (hCG), and, because hCG-stimulated androgen production in utero is crucial...... for normal testicular descent, exposure to COX inhibitors at vulnerable times during gestation may impair testicular descent. We examined whether prenatal exposure to acetaminophen, ibuprofen, and acetylsalicylic acid was associated with increased occurrence of cryptorchidism....

  4. Urethral metastasis from non-seminomatous germ cell tumor: a case report

    OpenAIRE

    Joffe Johnathan; Chilka Sameer; Wah Tze; Agarwal Vijay; Stark Dan

    2011-01-01

    Abstract Introduction We present a case of nonseminomatous germ cell tumor of the testes with acute urinary retention secondary to urethral metastasis. This presentation, and similar cases of urethral metastasis from this tumor, have not been reported previously. Case presentation A 35-year-old Caucasian man presented to hospital with a history of acute urinary retention. On examination he was found to have right testicular enlargement with raised β-human chorionic gonadotrophin, serum α-feto...

  5. Development of a protoplast based transformation system for genetic engineering of oil palm

    OpenAIRE

    Mat Yunus, Abdul Masani

    2013-01-01

    The major aim of the thesis was to develop the prerequisites for efficient genetic engineering of oil palm by DNA microinjection with the long-term objective to generate transgenic oil palm producing recombinant proteins, PIPP (a chimeric antibody against human chorionic gonadotropin; hCG), D12 (a human antibody against dental carries) and HSA (human serum albumin). The products will be synthesized in the leaf, mesocarp and kernel tissues of oil palm with the respects of plants must be stable...

  6. Localization and the possible role of plasminogen activators and inhibitors in early stages of placentation

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The distribution of mRNAs of tissue type (t) and urokinase type (u) plasminogen activator (PA) plus their corresponding inhibitors, type-1 (PAI-1) and type-2 (PAI-2) have been studied in the tissues of human first and second trimester placentae by in situ hybridization. The results show that: (ⅰ) All the molecules, tPA, uPA, PAI-1 and PAI-2, were identified in the blood vessels, the majority of extravillous trophoblastic cells of the decidual layer between Rohr's and Nitabuch's stria and in the trophoblast cells lining the chorionic plate, basal plate, intercotyledonary septae and cytotrophoblast cells of the chorionic villous tree. (ⅱ) No expression of such probes was observed in the basal and chorionic plate, glandular cells of the decidua, the septal tissues or the villous core mesenchyme. The co-distribution of the molecules observed suggests that the co-ordinated expression of the activators and inhibitors in various cells of the placental tissue may play a role in angiogenesis related to conversion of spiral arteries into utero-placental arteries and establishment of a chorio-decidual blood flow during early stages of placentation.

  7. A re-assessment of biochemical marker distributions in T21 affected and unaffected twin pregnancies in the first trimester

    DEFF Research Database (Denmark)

    Madsen, Helen Nordahl; Ball, Susan; Wright, Dave;

    2011-01-01

    for chromosomal abnormalities such as trisomy 21 in twin pregnancies. METHODS: 4843 unaffected and 47 trisomy 21-affected twin pregnancies were included in the study. Chorionicity-specific medians were generated for PAPP-A and free β-hCG from gestational ages 8 to 14 weeks. Multiple of the median values for each...... increased the detection rate for fetal trisomy 21 in dizygotic twin pregnancies from 78 to 90%, and decreased the false-positive rate from 8.0 to 5.9%. CONCLUSION: Generation of chorionicity-specific medians for the biochemical markers and their use in risk assessment can improve the performance of first...... show a gestational age-specific increase relative to singleton medians. Allowing for gestation and chorionicity, twin pregnancies affected with trisomy 21 had higher levels of free β-hCG and lower levels of PAPP-A. Adding biochemistry into the risk assessment using a fixed risk cut-off of 1 in 100...

  8. Hepatosplenomegaly Associated with Transient Abnormal Myelopoiesis in Down Syndrome: An Autopsy Case of a Stillborn Fetus

    Directory of Open Access Journals (Sweden)

    Michiko Yuki

    2015-06-01

    Full Text Available A 38-year-old primiparous mother (gravida 1, para 0 at 27 weeks and 6 days' gestation reported that fetal movements had been absent for 6 days. All serological markers for infection were negative. Chorionic villus sampling at stillbirth delivery revealed trisomy 21 (47, XX, +21, indicative of Down syndrome. The macerated baby was female and weighed 1,290 g. There was no evidence of hydrops fetalis. Proliferating blast cells expressing megakaryoblastic/megakaryocytic antigen CD61 were mainly seen within the vessels, and some cells infiltrated outside of the vessels in almost all organs. Vessels of the umbilical cord and chorionic villi were filled with proliferating blast cells, but the blast cells were not apparent in the bone marrow. The diagnosis of transient abnormal myelopoiesis in Down syndrome was made. Hepatomegaly (64.5 g was due to congestion and infiltration of CD61-positive blast cells within the vascular lumina and expanding outside the lumina accompanied by fibrotic change. The cause of death was attributed to liver insufficiency caused by liver fibrosis. An umbilical cord and chorionic villi examination may be helpful in the diagnosis of transient abnormal myelopoiesis when post-mortem examination is not permitted.

  9. Zika Virus Targets Different Primary Human Placental Cells, Suggesting Two Routes for Vertical Transmission.

    Science.gov (United States)

    Tabata, Takako; Petitt, Matthew; Puerta-Guardo, Henry; Michlmayr, Daniela; Wang, Chunling; Fang-Hoover, June; Harris, Eva; Pereira, Lenore

    2016-08-10

    Zika virus (ZIKV) infection during pregnancy is linked to severe birth defects, but mother-to-fetus transmission routes are unknown. We infected different primary cell types from mid- and late-gestation placentas and explants from first-trimester chorionic villi with the prototype Ugandan and a recently isolated Nicaraguan ZIKV strain. ZIKV infects primary human placental cells and explants-cytotrophoblasts, endothelial cells, fibroblasts, and Hofbauer cells in chorionic villi and amniotic epithelial cells and trophoblast progenitors in amniochorionic membranes-that express Axl, Tyro3, and/or TIM1 viral entry cofactors. ZIKV produced NS3 and E proteins and generated higher viral titers in amniotic epithelial cells from mid-gestation compared to late-gestation placentas. Duramycin, a peptide that binds phosphatidylethanolamine in enveloped virions and precludes TIM1 binding, reduced ZIKV infection in placental cells and explants. Our results suggest that ZIKV spreads from basal and parietal decidua to chorionic villi and amniochorionic membranes and that targeting TIM1 could suppress infection at the uterine-placental interface. PMID:27443522

  10. Triploidy--Observations in 154 Diandric Cases.

    Directory of Open Access Journals (Sweden)

    Nanna Brink Scholz

    Full Text Available Hydatidiform moles (HMs are abnormal human pregnancies with vesicular chorionic villi, imposing two clinical challenges; miscarriage and a risk of gestational trophoblastic neoplasia (GTN. The parental type of most HMs are either diandric diploid (PP or diandric triploid (PPM. We consecutively collected 154 triploid or near-triploid samples from conceptuses with vesicular chorionic villi. We used analysis of DNA markers and/or methylation sensitive-MLPA and collected data from registries and patients records. We performed whole genome SNP analysis of one case of twinning (PP+PM.In all 154 triploids or near-triploids we found two different paternal contributions to the genome (P1P2M. The ratios between the sex chromosomal constitutions XXX, XXY, and XYY were 5.7: 6.9: 1.0. No cases of GTN were observed. Our results corroborate that all triploid human conceptuses with vesicular chorionic villi have the parental type P1P2M. The sex chromosomal ratios suggest approximately equal frequencies of meiosis I and meiosis II errors with selection against the XYY conceptuses or a combination of dispermy, non-disjunction in meiosis I and meiosis II and selection against XYY conceptuses. Although single cases of GTN after a triploid HM have been reported, the results of this study combined with data from previous prospective studies estimate the risk of GTN after a triploid mole to 0% (95% CI: 0-1,4%.

  11. Placental mesenchymal dysplasia: chronological observation of placental images during gestation and review of the literature.

    Science.gov (United States)

    Ohira, Satoshi; Ookubo, Nao; Tanaka, Kyoko; Takatsu, Akiko; Kobara, Hisanori; Kikuchi, Norihiko; Ohya, Ayumi; Kanai, Makoto; Shiozawa, Tanri

    2013-01-01

    Placental mesenchymal dysplasia (PMD) is characterized by multiple hypoechoic vesicles which are similar to molar changes in the placenta; however, the process of such morphological changes of PMD during pregnancy has not been fully understood. We performed a review of all PMD cases published in English and identified 49 articles including 110 cases. With regard to the gestational age at which the multicystic pattern was seen, approximately 70% of cases were diagnosed at 13-20 weeks of gestation. Another characteristic feature of PMD is varicose dilation of fetal chorionic vessels. As many as 90% of cases were diagnosed as placenta with dilated fetal chorionic vessels in the third trimester. We also report a case of PMD which was found at 10 weeks of gestation according to ultrasonic molar patterns. Serial observations of the placenta using ultrasound and magnetic resonance imaging revealed that multicystic lesions became smaller after 23 weeks. In contrast, dilated placental vessels on the fetal side became apparent at 38 weeks. The present review highlights that placental vesicular lesions of PMD may precede dilation of fetal chorionic vessels during pregnancy. It also indicates the potential of a gradual reduction in size of PMD's placental vesicular lesions by serial study of placental images.

  12. Human reproduction functions: Evaluation with radiobioassay

    International Nuclear Information System (INIS)

    Many studies reveal that the ovary is capable of responding to an adequate gonadotropic stimulus long before menarche. Similarly, the pituitary is capable of producing gonadotropins in response to an adequate hypothalamic signal before menarche. Recent studies in the primate confirm that the hypothalamus signals are temporarily different before menarche as compared to the reproductive years, so that if the luteotropic hormone (LRH) stimulus is pulsed to the pituitary at the required time sequence, the follicle stimulating hormone (FSH) and the luteinizing hormone (LH) as well as ovulation can be achieved even in the immature monkey. HPL is another hormone produced by the syncytiotrophoblast that is also used to identify pregnancies at a higher risk of fetal demise. It shares structural similarities with the human growth hormone (hGH) and PRL. HPL is diabetogenic. Its effect is mediated through glucose metabolism. Circulating HPL is elevated during multiple gestations. Its circulating levels in fact correlate with the fetoplacental mass. It has a short half-life and the larger the fetoplacental mass, the higher the HPL level. Hyperglycemic states are associated with a decrease in HPL levels, and hypoglycemia is associated with elevated levels of HPL. RIA through the measurement of HPL has helped in the management of the high-risk fetus and its mother. Through RIA other fetoplacental hormones are identifiable and their levels are obtainable. There is a human chorionic ACTH, a human chorionic TSH, and a human chorionic PRL. These can be involved in health and disease

  13. Placentation in mammals: Definitive placenta, yolk sac, and paraplacenta.

    Science.gov (United States)

    Carter, A M; Enders, A C

    2016-07-01

    An overview is given of variations in placentation with particular focus on yolk sac, paraplacenta, and other structures important to histotrophic nutrition. The placenta proper varies in general shape, internal structure, and the number of tissues in the interhemal barrier. Yolk sac membranes persist to term in insectivores, colugos, rodents, and lagomorphs. In the latter two orders, they are of known importance for maternal-fetal transfer of antibodies, vitamins, lipids, and proteins. The detached yolk sac of bats is also active throughout gestation. A vascular paraplacenta, or smooth chorioallantois, has known functions in ruminants and carnivores and is found in several other orders of mammal where its function has yet to be explored. In human gestation, the chorion (avascular chorioallantois) is important for hormone synthesis. The true chorion of squirrels and hedgehogs is avascular but may nevertheless allow transfer from mother to fetus through the exocelom. Hemophagous areas with columnar trophoblast are paraplacental structures in carnivores and elephants but occur also within the placenta as in hyenas and moles. In shrews, it is the yolk sac that ingests and processes red cells. Areolas and chorionic vesicles are other structures important for absorption of uterine secretions and ingestion of cellular debris. In conclusion, we find that paraplacental structures, while showing less variation than the placenta proper, contribute not just to the integrity of overall placentation, but in various ways to maternal-fetal interrelationships. PMID:27155730

  14. Impairment of Angiogenic Sphingosine Kinase-1/Sphingosine-1-Phosphate Receptors Pathway in Preeclampsia

    Science.gov (United States)

    Dobierzewska, Aneta; Palominos, Macarena; Sanchez, Marianela; Dyhr, Michael; Helgert, Katja; Venegas-Araneda, Pia; Tong, Stephen; Illanes, Sebastian E.

    2016-01-01

    Preeclampsia (PE), is a serious pregnancy disorder characterized in the early gestation by shallow trophoblast invasion, impaired placental neo-angiogenesis, placental hypoxia and ischemia, which leads to maternal and fetal morbidity and mortality. Here we hypothesized that angiogenic sphingosine kinase-1 (SPHK1)/sphingosine-1-phosphate (S1P) receptors pathway is impaired in PE. We found that SPHK1 mRNA and protein expression are down-regulated in term placentae and term chorionic villous explants from patients with PE or severe PE (PES), compared with controls. Moreover, mRNA expression of angiogenic S1PR1 and S1PR3 receptors were decreased in placental samples of PE and PES patients, whereas anti-angiogenic S1PR2 was up-regulated in chorionic villous tissue of PES subjects, pointing to its potential atherogenic and inflammatory properties. Furthermore, in in vitro (JAR cells) and ex vivo (chorionic villous explants) models of placental hypoxia, SPHK1 mRNA and protein were strongly up-regulated under low oxygen tension (1% 02). In contrast, there was no change in SPHK1 expression under the conditions of placental physiological hypoxia (8% 02). In both models, nuclear protein levels of HIF1A were increased at 1% 02 during the time course, but there was no up-regulation at 8% 02, suggesting that SPHK1 and HIF1A might be the part of the same canonical pathway during hypoxia and that both contribute to placental neovascularization during early gestation. Taken together, this study suggest the SPHK1 pathway may play a role in the human early placentation process and may be involved in the pathogenesis of PE. PMID:27284992

  15. The cytoplasmic 60 kDa progesterone receptor isoform predominates in the human amniochorion and placenta at term

    Directory of Open Access Journals (Sweden)

    Bell Stephen C

    2009-03-01

    Full Text Available Abstract Background The mechanism that initiates human parturition has been proposed to be 'functional progesterone withdrawal' whereby the 116 kDa B-isoform of the progesterone receptor (PR-B switches in favour of the 94 kDa A-isoform (PR-A in reproductive tissues. Recently, other PR isoforms, PR-S, PR-C and PR-M generated from the same gene have been identified and partially characterised. Methods and Results Using immunohistochemical, western blotting and RT-PCR techniques, evidence is provided that indicates the major PR isoform present in human term fetal membranes (amnion and chorion and syncytiotrophoblast of the placenta is neither of the classical nuclear PR-B or PR-A isoforms but is the N-terminally truncated 60 kDa PR-C isoform. Evidence is also provided that this 60 kDa isoform resides in the cytoplasm of the expressing cell types. Data are also presented to show that PR-B, PR-A and PR-S isoforms are essentially absent from the amnion and chorion, whereas PR isoforms A, B, C and S are all present in the decidua, with PR-A being the major isoform. The syncytiotrophoblast of the placenta contains the cytoplasmic 60 kDa isoform, but not isoforms PR-A, PR-B or PR-S. Conclusion The major PR isoform in the amnion, chorion and placenta is a 60 kDa protein that could be PR-C, suggesting that the cytoplasmic isoform has a specific role in extra-embryonic tissues and may be involved in the regulation of human parturition.

  16. Olfactomedin 1 Deficiency Leads to Defective Olfaction and Impaired Female Fertility.

    Science.gov (United States)

    Li, Rong; Diao, Honglu; Zhao, Fei; Xiao, Shuo; El Zowalaty, Ahmed E; Dudley, Elizabeth A; Mattson, Mark P; Ye, Xiaoqin

    2015-09-01

    Olfactomedin 1 (OLFM1) is a glycoprotein highly expressed in the brain. Olfm1(-/-) female mice were previously reported to have reduced fertility. Previous microarray analysis revealed Olfm1 among the most highly upregulated genes in the uterine luminal epithelium upon embryo implantation, which was confirmed by in situ hybridization. We hypothesized that Olfm1 deficiency led to defective embryo implantation and thus impaired fertility. Indeed, Olfm1(-/-) females had defective embryo implantation. However, Olfm1(-/-) females rarely mated and those that mated rarely became pregnant. Ovarian histology indicated the absence of corpora lutea in Olfm1(-/-) females, indicating defective ovulation. Superovulation using equine chorionic gonadotropin-human chorionic gonadotropin rescued mating, ovulation, and pregnancy, and equine chorionic gonadotropin alone rescued ovulation in Olfm1(-/-) females. Olfm1(-/-) females had a 13% reduction of hypothalamic GnRH neurons but comparable basal serum LH levels and GnRH-induced LH levels compared with wild-type controls. These results indicated no obvious local defects in the female reproductive system and a functional hypothalamic-pituitary-gonadal axis. Olfm1(-/-) females were unresponsive to the effects of male bedding stimulation on pubertal development and estrous cycle. There were 41% fewer cFos-positive cells in the mitral cell layer of accessory olfactory bulb upon male urine stimulation for 90 minutes. OLFM1 was expressed in the main and accessory olfactory systems including main olfactory epithelium, vomeronasal organ, main olfactory bulb, and accessory olfactory bulb, with the highest expression detected in the axon bundles of olfactory sensory neurons. These data demonstrate that defective fertility in Olfm1(-/-) females is most likely a secondary effect of defective olfaction. PMID:26107991

  17. Productive infection of bovine papillomavirus type 2 in the placenta of pregnant cows affected with urinary bladder tumors.

    Directory of Open Access Journals (Sweden)

    Sante Roperto

    Full Text Available Papillomaviruses (PVs are believed to be highly epitheliotropic as they usually establish productive infections within stratified epithelia. In vitro, various PVs appear to complete their entire life-cycle in different trophoblastic cell lines. In this study, infection by and protein expression of bovine papillomavirus type 2 (BPV-2 in the uterine and chorionic epithelium of the placenta has been described in four cows suffering from naturally occurring papillomavirus-associated urothelial bladder tumors. E5 oncoprotein was detected both by Western blot analysis and immunohistochemically. It appears to be complexed and perfectly co-localized with the activated platelet-derived growth factor ß receptor (PDGFßR by laser scanning confocal microscopy. The activated PDGFßR might be involved in organogenesis and neo-angiogenesis rather than in cell transformation during pregnancy. The major capsid protein, L1, believed to be only expressed in productive papillomavirus infection has been detected by Western blot analysis. Immunohistochemical investigations confirmed the presence of L1 protein both in the cytoplasm and nuclei of cells of the uterine and chorionic epithelium. Trophoblastic cells appear to be the major target for L1 protein expression. Finally, the early protein E2, required for viral DNA replication and known to be expressed during a productive infection, has been detected by Western blot and immunohistochemically. Electron microscopic investigations detected viral particles in nuclei of uterine and chorionic epithelium. This study shows that both active and productive infections by BPV-2 in the placenta of pregnant cows can occur in vivo.

  18. Olfactomedin 1 Deficiency Leads to Defective Olfaction and Impaired Female Fertility.

    Science.gov (United States)

    Li, Rong; Diao, Honglu; Zhao, Fei; Xiao, Shuo; El Zowalaty, Ahmed E; Dudley, Elizabeth A; Mattson, Mark P; Ye, Xiaoqin

    2015-09-01

    Olfactomedin 1 (OLFM1) is a glycoprotein highly expressed in the brain. Olfm1(-/-) female mice were previously reported to have reduced fertility. Previous microarray analysis revealed Olfm1 among the most highly upregulated genes in the uterine luminal epithelium upon embryo implantation, which was confirmed by in situ hybridization. We hypothesized that Olfm1 deficiency led to defective embryo implantation and thus impaired fertility. Indeed, Olfm1(-/-) females had defective embryo implantation. However, Olfm1(-/-) females rarely mated and those that mated rarely became pregnant. Ovarian histology indicated the absence of corpora lutea in Olfm1(-/-) females, indicating defective ovulation. Superovulation using equine chorionic gonadotropin-human chorionic gonadotropin rescued mating, ovulation, and pregnancy, and equine chorionic gonadotropin alone rescued ovulation in Olfm1(-/-) females. Olfm1(-/-) females had a 13% reduction of hypothalamic GnRH neurons but comparable basal serum LH levels and GnRH-induced LH levels compared with wild-type controls. These results indicated no obvious local defects in the female reproductive system and a functional hypothalamic-pituitary-gonadal axis. Olfm1(-/-) females were unresponsive to the effects of male bedding stimulation on pubertal development and estrous cycle. There were 41% fewer cFos-positive cells in the mitral cell layer of accessory olfactory bulb upon male urine stimulation for 90 minutes. OLFM1 was expressed in the main and accessory olfactory systems including main olfactory epithelium, vomeronasal organ, main olfactory bulb, and accessory olfactory bulb, with the highest expression detected in the axon bundles of olfactory sensory neurons. These data demonstrate that defective fertility in Olfm1(-/-) females is most likely a secondary effect of defective olfaction.

  19. Electro-microinjection of fish eggs with an immobile capillary electrode.

    Science.gov (United States)

    Shirakashi, Ryo; Yasui, Tatsuo; Memmel, Simon; Sukhorukov, Vladimir L

    2015-11-01

    Microinjection with ultra-fine glass capillaries is widely used to introduce cryoprotective agents and other foreign molecules into animal cells, oocytes, and embryos. The fragility of glass capillaries makes difficult the microinjection of fish eggs and embryos, which are usually protected by a hard outer shell, called the chorion. In this study, we introduce a new electromechanical approach, based on the electropiercing of fish eggs with a stationary needle electrode. The electropiercing setup consists of two asymmetric electrodes, including a μm-scaled nickel needle placed opposite to a mm-scaled planar counter-electrode. A fish egg is immersed in low-conductivity solution and positioned between the electrodes. Upon application of a short electric pulse of sufficient field strength, the chorion is electroporated and the egg is attracted to the needle electrode by positive dielectrophoresis. As a result, the hard chorion and the subjacent yolk membrane are impaled by the sharp electrode tip, thus providing direct access to the egg yolk plasma. Our experiments on early-stage medaka fish embryos showed the applicability of electro-microinjection to fish eggs measuring about 1 mm in diameter. We optimized the electropiercing of medaka eggs with respect to the field strength, pulse duration, and conductivity of bathing medium. We microscopically examined the injection of dye solution into egg yolk and the impact of electropiercing on embryos' viability and development. We also analyzed the mechanisms of electropiercing in comparison with the conventional mechanical microinjection. The new electropiercing method has a high potential for automation, e.g., via integration into microfluidic devices, which would allow a large-scale microinjection of fish eggs for a variety of applications in basic research and aquaculture. PMID:26649129

  20. Primary Gastric Chorioadenocarcinoma.

    Science.gov (United States)

    Baraka, Bahaaeldin A; Al Kharusi, Suad S; Al Bahrani, Bassim J; Bhathagar, Gunmala

    2016-09-01

    Primary gastric chorioadenocarcinoma (PGC) is a rare and rapidly invasive tumor. Choriocarcinoma is usually known to be of endometrial origin and gestational; however, it has been reported in other extragenital organs, such as the gall bladder, prostate, lung, liver, and the gastrointestinal tract. Human chorionic gonadotropin related neoplasms of the stomach are seldom discussed in the literature. We report a case of PGC in a 56-year-old man treated with a standard non-gestational choriocarcinoma chemotherapy regimen, EMA/CO (etoposide, methotrexate, actinomycin D, cyclophosphamide, vincristine), with a complete response and good tolerability. PMID:27602194

  1. Growth and development in Syrian Awassi ewes during patterns of ovarian follicular different reproductive stages monitored by laparoscopy

    International Nuclear Information System (INIS)

    An in situ study was conducted on Syrian Awassi ewes, inside and outside the breeding season, untreated or treated with vaginal sponges together with or without equine chorionic gonadotropin aiming at monitoring the ovaries' diameters, number of different follicular categories, number, diameters and lifespan of corpora lutea in each ovary using a laparoscope and finding out the relationship among these parameters and progesterone concentration. For the first time, it has been possible to characterise the Syrian Awassi sheep at the ovarian follicles level during different reproductive stages using a laparoscope. (author)

  2. Pituitary and placental hormone levels in pseudocyesis.

    Science.gov (United States)

    Osotimehin, B O; Ladipo, O A; Adejuwon, C A; Otolorin, E O

    1981-10-01

    Twelve patients with clinical features of pseudocyesis were divided into two groups according to the presence or absence of galactorrhea. The mean serum prolactin level of patients with galactorrhea was significantly higher than the normal values of the patients without galactorrhea. The mean serum levels of luteinizing hormone and follicle-stimulating hormone were markedly elevated in patients without galactorrhea. This was especially true of luteinizing hormone. Serum levels of human chorionic gonadotropin were undetectable in all patients. The significance of these observations is discussed.

  3. Effects of Dietary Plant-Origin Glucosylceramide on Bowel Inflammation in DSS-Treated Mice.

    Science.gov (United States)

    Arai, Katsuhito; Mizobuchi, Yu; Tokuji, Yoshihiko; Aida, Kazuhiko; Yamashita, Shinji; Ohnishi, Masao; Kinoshita, Mikio

    2015-01-01

    The effects of dietary plant-origin glucosylceramide (GlcCer) on symptoms similar to those of inflammatory bowel diseasewere investigated in dextran sulfate sodium salt (DSS)-treated mice. Dietary GlcCer suppressed decreases in body weight due to DSS administration. To determine its effects on the colon, we examined its surface under a microscope following toluidine blue staining. Dietary GlcCer decreased DSS-induced chorionic crypt injury and elevated myeloperoxidase levels. Moreover, dietary GlcCer significantly suppressed the production of cytokines by the intestinal mucosa. These results provide evidence for the suppression of DSS-induced inflammation by dietary GlcCer.

  4. Improving the luteal phase after ovarian stimulation

    DEFF Research Database (Denmark)

    Andersen, Claus Yding; Vilbour Andersen, K

    2014-01-01

    The human chorionic gonadotrophin (HCG) trigger used for final follicular maturation in connection with assisted reproduction treatment combines ovulation induction and early luteal-phase stimulation of the corpora lutea. The use of a gonadotrophin-releasing hormone agonist (GnRHa) for final...... follicular maturation has, however, for the first time allowed a separation of the ovulatory signal from the early luteal-phase support. This has generated new information that may improve the currently employed luteal-phase support. Thus, combined results from a number of randomized controlled trials using...

  5. Preovulatory progesterone concentration associates significantly to follicle number and LH concentration but not to pregnancy rate

    DEFF Research Database (Denmark)

    Yding Andersen, Claus; Bungum, Leif; Nyboe Andersen, Anders;

    2011-01-01

    Using data from a large prospective randomized controlled trial that evaluated the effect of recombinant LH (rLH)co-administration for ovarian stimulation, the present study assessed whether progesterone concentration on the day of human chorionic gonadotrophin (HCG) administration was associated...... with pregnancy outcome. Progesterone concentration was measured on stimulation day 1 and on the day of HCG administration in 475 patients who underwent IVF/intracytoplasmic sperm injection treatment following ovarian stimulation with gonadotrophin-releasing hormone (GnRH) agonist and recombinant FSH...

  6. Choriocarcinoma syndrome を来した性腺外胚細胞腫瘍に対してModified BEP レジメンによる導入化学療法が奏効した1例

    OpenAIRE

    大島, 純平; 植村, 元秀; 加藤, 大悟; 永原, 啓; 木内, 寛; 辻村, 晃; 野々村, 祝夫

    2014-01-01

    We present a case study of a 46-year-old man with extra gonadal germ cell tumor with multiple lung metastases and very high levels (324, 100 mIU/ml) of the tumor marker human chorionic gonadotropin (hCG). He underwent chemotherapy with VP-16, ifosfamide and cisplatinum regimen, but on day 2, he noticed strong dyspnea. A chest X-ray showed bilateral infiltration of the lungs, and he was diagnosed with acute respiratory distress syndrome (ARDS) from choriocarcinoma syndrome. After ARDS improved...

  7. Placentation in mammals

    DEFF Research Database (Denmark)

    Carter, A M; Enders, A C

    2016-01-01

    An overview is given of variations in placentation with particular focus on yolk sac, paraplacenta, and other structures important to histotrophic nutrition. The placenta proper varies in general shape, internal structure, and the number of tissues in the interhemal barrier. Yolk sac membranes....... Areolas and chorionic vesicles are other structures important for absorption of uterine secretions and ingestion of cellular debris. In conclusion, we find that paraplacental structures, while showing less variation than the placenta proper, contribute not just to the integrity of overall placentation...

  8. Detection of Human Parvovirus B19 Nonstrutural Protein DNA by Nested-Polymerase Chain Reaction in Gravida Serum and Pregnant Tissues

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A new nested-polymerase chain reaction (nested-PCR) assay was developed to detect human parvovirus B19 DNA corresponding to the nonstructural protein in clinical specimens in a routine diagnostic laboratory. The sensitivity of this highly specific assay was up to 0. 005 fg of B19 DNA. Parvovirus B19 was identified in sera of 20 pregnant women with abnormal pregnant outcome. Among these 20 cases, intrauterine parvovirus infection did exist in 7 pregnant women because parvovirus B19 DNA was detected in the pregnant tissues of them such as placenta tissues,chorionic villi, amniotic fluid, fetal spleen, liver and abdominal fluids.

  9. Screening and Testing in Multiples.

    Science.gov (United States)

    Evans, Mark I; Andriole, Stephanie; Evans, Shara M

    2016-06-01

    The choice of screening or invasive procedure in twin pregnancies is a personal choice of whether the patient wishes to take a small risk of having a baby with a serious disorder versus a small risk of having a complication because she wishes to avoid that. How to interpret such risks has profound effects on the perceived value of techniques, either leading to a decision to screening or going directly to chorionic villus sampling. There are profound issues surrounding the data and the interpretation of the data. No single short review can exhaustively examine all of the issues. PMID:27235913

  10. Paraneoplastic precocious puberty and excessively rapid somatic growth associated with pediatric malignant hepatic tumor: 1 report of 2 cases

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Ok Hwa; Bahk, Yong Whee [Cathalic University College of Medicine, Seoul (Korea, Republic of)

    1990-04-15

    Sexual precocity in a 28-months-old boy and markedly accelerated skeletal growth with large body in a 5 years and 5 months-old-girl are reported. The former resulted from human chorionic gonadotropin-producing hepatoblastoma and the latter from cerebral gigantism associated with hepatoma. These two different disorders are discussed on the common basis of rare association of malignant hepatic tumors with precocious sexual and / or somatic growth. The clinical manifestations, chemical abnormalities, and the radiologic findings are presented with a brief review of the literature.

  11. Prenatal diagnosis of Morquio disease type A using a simple fluorometric enzyme assay.

    Science.gov (United States)

    Zhao, H; Van Diggelen, O P; Thoomes, R; Huijmans, J; Young, E; Mazurczak, T; Kleijer, W J

    1990-02-01

    A new fluorogenic substrate, 4 methylumbelliferyl beta-D-6-sulphogalactoside, was used for the assay of galactose-6-sulphate sulphatase activity in chorionic villi, cultured villus cells, and amniocytes. The fluorometric assay is much more convenient than the conventional assay using radiolabelled, sulphated oligosaccharides. Both types of substrate were used in the prenatal diagnosis of three pregnancies at risk for Morquio type A disease using amniocytes. These enzyme tests, as well as electrophoresis of glycosaminoglycans in the amniotic fluid, indicated affected fetuses in two pregnancies and a non-affected fetus in one. PMID:2111546

  12. NCBI nr-aa BLAST: CBRC-PCAP-01-0876 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available AltName: Full=LH/CG-R; AltName: Full=LSH-R; AltName: Full=Luteinizing hormone receptor; Flags: Precursor pir||I77463 lute...CBRC-PCAP-01-0876 ref|NP_037110.1| luteinizing hormone/choriogonadotropin receptor ...| choriogonadotropic hormone receptor [Rattus norvegicus] gb|AAA41529.1| luteinizing hormone receptor [Rattu...s norvegicus] gb|AAB22680.1| luteinizing hormone/chorionic gonadotropin receptor [Rattus sp.] gb|EDM02617.1| lute

  13. NCBI nr-aa BLAST: CBRC-MMUR-01-1524 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available AltName: Full=LH/CG-R; AltName: Full=LSH-R; AltName: Full=Luteinizing hormone receptor; Flags: Precursor pir||I77463 lute...CBRC-MMUR-01-1524 ref|NP_037110.1| luteinizing hormone/choriogonadotropin receptor ...| choriogonadotropic hormone receptor [Rattus norvegicus] gb|AAA41529.1| luteinizing hormone receptor [Rattu...s norvegicus] gb|AAB22680.1| luteinizing hormone/chorionic gonadotropin receptor [Rattus sp.] gb|EDM02617.1| lute

  14. Cisplatinum dose dependent response in germ cell cancer evaluated by tumour marker modelling

    DEFF Research Database (Denmark)

    Carl, J; Christensen, T B; von der Maase, H

    1992-01-01

    This study presents an analysis on longitudinal tumour marker series in twenty-two patients with non-seminomatous germ cell cancers treated with cisplatinum (DDP) based combination chemotherapy. Series of alphafoetoprotein (AFP), human chorionic gonadotrophin (HCG) and lactate dehydrogenase (LDH...... faster than AFP producing cells, and were 3-5-fold more sensitive to the chemotherapy given than AFP producing cells. Treatment response versus DDP dose appeared to be bi-phasic, but with no significant change in treatment efficiency within the given range of DDP doses....

  15. In vitro fertility rate of 129 strain is improved by Buserelin (GnRH) administration prior to superovulation

    OpenAIRE

    Vasudevan, K; Sztein, J M

    2012-01-01

    The 129 mice are well recognized for their low fertility and it is speculated that this lack of fertility may be due to oocyte condition. In this study we investigated superovulation regimens for 129S1/SvImJ mouse strain to improve the oocyte quality and fertility rate of in vitro fertilization (IVF). Female mice were divided into four groups based on hormone and timing of injection. Group 1 received pregnant mare serum gonatotropin (PMSG) and 48 hours later human chorionic gonadotropin (hCG)...

  16. Mixed ovarian germ cell tumor composed of immature teratoma, yolk sac tumor and embryonal carcinoma.

    Science.gov (United States)

    Wang, Ying; Zhou, Feng; Qian, Zhida; Qing, Jiale; Zhao, Mengdam; Huang, Lili

    2014-11-01

    We report the case of a 19-year-old woman experiencing lower abdominal distension and pain. Laboratory tests indicated elevated serum levels of Alpha-Fetoprotein (AFP) and human Chorionic Gonadotropin (hCG). A large mass was detected in the abdomen by physical examination and by transvaginal ultrasonography. Exploratory laparotomy was performed, and a smooth-surfaced, spherical, solid tumor was found on the left ovary, measuring 11.5 x 9.9 x 6.9 cm. Histological evaluation revealed that the tumor consisted of a combination of immature teratoma, Yolk Sac Tumor, and embryonal carcinoma; this is a very rare combination in mixed germ cell tumors. PMID:25518772

  17. Autocorrelation and cross correlation between free hCGβ and PAPP-A in repeated sampling during first trimester of pregnancy

    DEFF Research Database (Denmark)

    Nørgaard, Pernille; Wright, Dave; Ball, Susan;

    2013-01-01

    Theoretically, repeated sampling of free β-human chorionic gonadotropin (hCGβ) and pregnancy associated plasma protein-A (PAPP-A) in the first trimester of pregnancy might improve performance of risk assessment of trisomy 21 (T21). To assess the performance of a screening test involving repeated...... measures of biochemical markers, correlations between markers must be estimated. The aims of this study were to calculate the autocorrelation and cross-correlation between hCGβ and PAPP-A in the first trimester of pregnancy and to investigate the possible impact of gestational age at the first sample...... and time between sampling on the correlation....

  18. Surface plasmon resonance imaging for parallelized detection of protein biomarkers

    Science.gov (United States)

    Piliarik, Marek; Párová, Lucie; Vaisocherová, Hana; Homola, Jiří

    2009-05-01

    We report a novel high-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of protein biomarkers. The biosensor is based on a high-performance SPR imaging sensor with polarization contrast and internal referencing which yields a considerably higher sensitivity and resolution than conventional SPR imaging systems (refractive index resolution 2 × 10-7 RIU). We combined the SPR imaging biosensor with microspotting to create an array of antibodies. DNA-directed protein immobilization was utilized for the spatially resolved attachment of antibodies. Using Human Chorionic Gonadotropin (hCG) as model protein biomarker, we demonstrated the potential for simultaneous detection of proteins in up to 100 channels.

  19. [Laparoscopic radical cystectomy and orthotopic urinary diversion in the male patient: technique].

    Science.gov (United States)

    Nuñez-Mora, C; Cabrera, P; García-Mediero, J M; de Fata, F Ramón; González, J; Angulo, J

    2011-04-01

    Radical cystectomy with extended pelvic lymphadenectomy remains the treatment of choice for muscle invasive bladder cancer and non-metastatic chorion-invasive high grade tumors resistant to treatment with intravesical chemotherapy. During the last decade the procedure has been refined and we have acquired the skills necessary to perform it using the laparoscopic approach. In this way, the oncologic and functional outcomes obtained can be compared to those of its open counterpart. This article describes in detail the technique of radical cystoprostatectomy and urinary diversion in the male patient conducted by our group in an attempt to improve the knowledge and spread of this always difficult procedure. PMID:21487170

  20. High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains—selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG

    OpenAIRE

    Blanchard, Véronique; Gadkari, R.P; George, A.V.E.; Roy, R; Gerwig, G J; Leeflang, B.R.; Dighe, R R; Boelens, R.; Kamerling, J P

    2008-01-01

    The methylotrophic yeast Pichia pastoris is widely used for the production of recombinant glycoproteins. With the aim to generate biologically active 15N-labeled glycohormones for conformational studies focused on the unravelling of the NMR structures in solution, the P. pastoris strains GS115 and X-33 were explored for the expression of human chorionic gonadotropin (phCG) and human follicle-stimulating hormone (phFSH). In agreement with recent investigations on the N-glycosylation of phCG, p...

  1. Dynamics of extracellular matrix in ovarian follicles and corpora lutea of mice

    OpenAIRE

    Irving-Rodgers, Helen F.; Hummitzsch, Katja; Murdiyarso, Lydia S.; Bonner, Wendy M.; Sado, Yoshikazu; Ninomiya, Yoshifumi; Couchman, John R.; Sorokin, Lydia M; Rodgers, Raymond J.

    2009-01-01

    Despite the mouse being an important laboratory species, little is known about changes in its extracellular matrix (ECM) during follicle and corpora lutea formation and regression. Follicle development was induced in mice (29 days of age/experimental day 0) by injections of pregnant mare’s serum gonadotrophin on days 0 and 1 and ovulation was induced by injection of human chorionic gonadotrophin on day 2. Ovaries were collected for immunohistochemistry (n=10 per group) on days 0, 2 and 5. Ano...

  2. Cri-du-chat syndrome: clinical profile and prenatal diagnosis.

    Directory of Open Access Journals (Sweden)

    Tullu M

    1998-10-01

    Full Text Available Prenatal diagnosis of cri-du-chat syndrome is described in 2 pregnancies. In Case 1, the mother was a balanced translocation carrier and had 2 previously affected off springs. Prenatal diagnosis by chorion villus sampling and cordocentesis was successful in diagnosing an affected conceptus and the pregnancy was electively terminated. Case 2 was referred for nonimmune foetal hydrops and cordocentesis revealed deletion 5p. This second case was noteworthy for the fact that deletion 5p has not been reported to cause foetal hydrops.

  3. Study on the immunosuppressants in the peripartum stages in sows

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The dynamic level of immunosuppressants such as alpha-fetoprotein(α-FP), gestation associated protein, progesterone, estradiol, chorionic gonadotropin, etc. of peripheral sera and the regulatory mechanism of cellular immunity in the peripartum stages in sows were determined by advanced radioimmunoassay (RIA) and immunochemistry methods in this study. The effects on lymphocyte cultivated in vitro by the immunosuppressants mentioned above were tested by cell culture technology, respectively. The results were as follows: (1) α-FP: The level of α-FP of peripheral sera in the stage of ante-parturition was slightly higher than that of post-parturition in sows, but it didn't show statistical difference. The concentration of α-FP of peripheral sera in conceptus sows had no obvious effect on lymphocyte cultivated in vitro, and it indicated that α-FP was not the major immunosuppressant in the pregnancy of sows. However, the α-FP, whose concentration was equal to that of amniotic fluid, had obvious inhibition on lymphocyte cultivated in vitro. (2) Gestation associated protein: The level of gestation associated protein was maintained highly (100-162 mg/L) in peripheral sera and amniotic fluid in the stage of ante-parturition in sows, then descended gradually after parturition and disappeared step by step 5-10 d after parturition. The gestation associated protein, whose concentration were equal to that of peripheral sera and amniotic fluid, were added to lymphocyte culture in vitro, and the effect on lymphocyte activity was not observed yet. This showed that gestation associated protein was not the major immunosuppressant in the peripartum stage in sows. (3) Estradiol and progesterone: Estradiol or progesterone at the level of sera in pregnant sows, could lower the rate of lymphocyte transformation and the level of cellular immunity. (4) Chorionic gonadotropin: The level of chorionic gonadotropin of peripheral sera in the stage of ante-parturition was slightly higher than

  4. The diagnostic potential of maternal plasma in detecting fetal diseases by DNA test

    Directory of Open Access Journals (Sweden)

    Saha Biswajit

    2004-01-01

    Full Text Available Conventionally, DNA based investigations for fetal diseases are done by chorionic villous sampling and amniocentesis. Both are invasive techniques. Recently, molecular diagnosis has also been made possible in early pregnancy from maternal blood which is noninvasive and advantageous. Most of the researches have tried to identify the Y chromosome marker(s to detect a male fetus and paternally inherited allele. This is currently helpful to detect a very few genetic disorders including Rh D status in Rh negative women in early pregnancy and preeclampsia a few weeks preceding the clinical onset. This is a potential area for prenatal diagnosis in future.

  5. Présence de réceptacles séminaux chez les Caméléons

    NARCIS (Netherlands)

    Saint Girons, H.

    1962-01-01

    La partie distale de l’oviducte des Caméléons montre, peu avant son débouché dans le cloaque, des réceptacles séminaux constitués par de simples tubes en doigt de gant, apparemment dépourvus d’activité sécrétrice, qui pénètrent dans le chorion. Des spermatozoïdes ont été trouvés en grand nombre dans

  6. Hormone Stories in Argentina.Obesity in children and early diagnosis of pregnancy in the 1930s and 1940s

    Directory of Open Access Journals (Sweden)

    Cecilia Rustoyburu

    2015-09-01

    Full Text Available This article inquires, from a gender perspective, knowledge production about hormones in Argentina, between the years 1930-1940. We understand that a historical analysis of scientific ideas and clinical practices allows explore how they involved on the social scenario which they belong, plus the institutional contexts where they are elaborated. We will focus on endocrinologist's Hospital de Niños de Buenos Aires perspective about obesity on childhood, and adipose genital síndrome, andearly diagnosis of pregnancy and chorionic gonadotropin hormone in the 1930s and 1940s

  7. Rate of Hepatitis B Virus Infection in Pregnant Women Determined by a Monoclonal Hepatitis B Surface Antigen Immunoassay

    OpenAIRE

    Gotstein, Melissa G.; Aide, Paula M.; Coleman, Paul F.; Sanborn, Mark R.

    2002-01-01

    The rate of HBsAg in 6,976 B-human chorionic gonadotropin (B-hCG)-positive specimens, as determined by the Auszyme Monoclonal assay (Abbott Laboratories, Abbott Park, Ill.), was 0.56% (39 of 6,986 repeatedly reactive [RR] and confirmed-positive specimens). All RR and confirmed specimens were hepatitis B virus positive by at least one additional test, yielding an assay specificity of 99.96%. The findings argue against unique attributes in the pregnant population that might produce inaccurate a...

  8. A unique scrotal extratesticular epidermod cyst attached to the seminal vesicles.

    Science.gov (United States)

    Sağlam, Hasan Salih; Kumsar, Sükrü; Köse, Osman; Adsan, Oztuğ

    2013-01-01

    A 46-year-old man was admitted with a scrotal long standing painless mass. The workup included physical examination, alpha-fetoprotein (αFP) and beta-human chorionic gonadotropin (β-hCG) analyses, scrotal ultrasound, magnetic resonance imaging and urethrocystoscopy. Surgical exploration revealed a separate mass between the testes extending superiorly with a thin stalk. It was dissected easily to the anterior aspect of the seminal vesicles and removed from the junction to the seminal vesicles. Pathology reported an epidermoid cyst. To our knowledge this is the first case of a scrotal extratesticular epidermoid cyst attached to the seminal vesicles.

  9. Rate of hepatitis B virus infection in pregnant women determined by a monoclonal hepatitis B surface antigen immunoassay.

    Science.gov (United States)

    Gotstein, Melissa G; Aide, Paula M; Coleman, Paul F; Sanborn, Mark R

    2002-09-01

    The rate of HBsAg in 6,976 B-human chorionic gonadotropin (B-hCG)-positive specimens, as determined by the Auszyme Monoclonal assay (Abbott Laboratories, Abbott Park, Ill.), was 0.56% (39 of 6,986 repeatedly reactive [RR] and confirmed-positive specimens). All RR and confirmed specimens were hepatitis B virus positive by at least one additional test, yielding an assay specificity of 99.96%. The findings argue against unique attributes in the pregnant population that might produce inaccurate assay results. PMID:12202601

  10. Choriocarcinoma presenting with thyrotoxicosis.

    Science.gov (United States)

    Sotello, David; Rivas, Ana Marcella; Test, Victor J; Lado-Abeal, Joaquin

    2016-01-01

    We describe a 26-year-old man with metastatic choriocarcinoma who presented with hyperthyroidism associated with elevated β-human chorionic gonadotropin (B-HCG) and respiratory failure secondary to diffuse lung metastasis. After the first cycle of chemotherapy, the concentration of B-HCG dramatically decreased and the patient became euthyroid, allowing us to discontinue antithyroid medications. The patient's hyperthyroidism was caused by stimulation of the thyroid gland by high B-HCG levels, as shown by the marked improvement of the patient's thyroid function panel after chemotherapy. PMID:26722165

  11. Growth and Development of the Uterus and Placenta of Superovulated Gilts

    OpenAIRE

    REVOLSON ALEXIUS MEGE; SYAHRUN HAMDANI NASUTION; NASTITI KUSUMORINI; WASMEN MANALU

    2007-01-01

    Forty eight gilts with average body weight of 107.83 + 5.08 kg were used in experiments to study the use of pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (hCG) as superovulation agent in gilts to increase piglet production. Four groups of twelve gilts were injected with PMSG dan hCG dose levels of 0, 600, 1200, and 1800 IU/gilt. Injections were conducted three days before estrus. During gestation, gilts were placed in colony pigpens. On days 15, 35, and 70 of ges...

  12. Microdissection testicular sperm extraction in patients with non-mosaic Klinefelter syndrome in programme in vitro fertilization: literature review and cases report

    Directory of Open Access Journals (Sweden)

    I. I. Vityazeva

    2014-12-01

    Full Text Available The article describes modern methods of examination, preoperative preparation and surgical treatment of infertility in men with non-mosaic form of Klinefelter syndrome (SK by microdissection method of testicular sperm retrieval. Men with SK responsive to hormonal therapy with aromatase inhibitors and human chorionic gonadotropin (НCG, have a greater chance of a favourable outcome of infertility treatment. Testicular volume, testosterone levels and test results with НCG are important prognostic factors in this group of patients. We present a clinical case of treatment of infertility in men with SK by microTESE (microdissection testicular sperm extraction.

  13. Microdissection testicular sperm extraction in patients with non-mosaic Klinefelter syndrome in programme in vitro fertilization: literature review and cases report

    Directory of Open Access Journals (Sweden)

    I. I. Vityazeva

    2014-01-01

    Full Text Available The article describes modern methods of examination, preoperative preparation and surgical treatment of infertility in men with non-mosaic form of Klinefelter syndrome (SK by microdissection method of testicular sperm retrieval. Men with SK responsive to hormonal therapy with aromatase inhibitors and human chorionic gonadotropin (НCG, have a greater chance of a favourable outcome of infertility treatment. Testicular volume, testosterone levels and test results with НCG are important prognostic factors in this group of patients. We present a clinical case of treatment of infertility in men with SK by microTESE (microdissection testicular sperm extraction.

  14. Hyperthyroidism in pregnancy

    DEFF Research Database (Denmark)

    Nygaard, Birte

    2015-01-01

    INTRODUCTION: Hyperthyroidism is characterised by high levels of serum thyroxine and triiodothyronine, and low levels of thyroid-stimulating hormone. The main causes of hyperthyroidism in pregnancy are Graves' disease and chorionic gonadotrophin (hCG)-mediated hyperthyroidism. METHODS AND OUTCOMES......: We conducted a systematic review and aimed to answer the following clinical question: What are the effects of antithyroid drug treatments for hyperthyroidism in pregnancy? We searched: Medline, Embase, The Cochrane Library, and other important databases up to June 2014 (Clinical Evidence reviews...

  15. Update on procedure-related risks for prenatal diagnosis techniques

    DEFF Research Database (Denmark)

    Tabor, Ann; Alfirevic, Zarko

    2010-01-01

    Introduction: As a consequence of the introduction of effective screening methods, the number of invasive prenatal diagnostic procedures is steadily declining. The aim of this review is to summarize the risks related to these procedures. Material and Methods: Review of the literature. Results: Data...... from randomised controlled trials as well as from systematic reviews and a large national registry study are consistent with a procedure-related miscarriage rate of 0.5-1.0% for amniocentesis as well as for chorionic villus sampling (CVS). In single-center studies performance may be remarkably good due...... invasive procedures calls for quality assurance and monitoring of operators' performance....

  16. Diagnosis and prediction of parental origin of triploidies by fetal nuchal translucency and maternal serum free beta-hCG and PAPP-A at 11-14 weeks of gestation

    DEFF Research Database (Denmark)

    Barken, Sidsel Svennekjær; Skibsted, L.; Jensen, Lars Nimb;

    2008-01-01

    consent, quantitative fluorescence polymerase chain reaction analysis was performed on the five cases where we received blood from both parents and tissue from fetuses. In four cases the origin of the triploidy was paternal and in one maternal, in accordance with previous findings in type I and type II......The study objective was to determine the parental origin of triploidy in relation to findings from early risk assessment in a combined screening program between 2004 and the end of 2006. Triploidy was diagnosed in six chorion villus samples and two samples from missed abortions. After informed...

  17. Characterization and histologic localization of human growth hormone-variant gene expression in the placenta.

    OpenAIRE

    Liebhaber, S A; Urbanek, M; Ray, J.; Tuan, R.S.; Cooke, N E

    1989-01-01

    The human growth hormone-variant (hGH-V) gene is one of five highly similar growth hormone-related genes clustered on the short arm of chromosome 17. Although the pattern of expression of the adjacent normal growth hormone (hGH-N) and chorionic somatomammotropin (hCS) genes in this cluster are well characterized, the expression of the hGH-V gene remains to be defined. In previous studies, we have demonstrated that the hGH-V gene is transcribed in the term placenta and expressed as two alterna...

  18. Interferon-gamma and tumor necrosis factor-alpha sensitize primarily resistant human endometrial stromal cells to Fas-mediated apoptosis

    DEFF Research Database (Denmark)

    Fluhr, Herbert; Krenzer, Stefanie; Stein, Gerburg M;

    2007-01-01

    of Fas by an agonistic anti-Fas antibody. Incubation of ESCs with the early embryonic signal human chorionic gonadotropin (hCG, CGB) does not influence their reaction to Fas stimulation. The sensitizing effect of IFN-gamma and TNF-alpha was accompanied by a significant upregulation of Fas and FLICE......The subtle interaction between the implanting embryo and the maternal endometrium plays a pivotal role during the process of implantation. Human endometrial stromal cells (ESCs) express Fas and the implanting trophoblast cells secrete Fas ligand (FASLG, FasL), suggesting a possible role for Fas...

  19. First Trimester Maternal Serum Screening Using Biochemical Markers PAPP-A and Free β-hCG for Down Syndrome, Patau Syndrome and Edward Syndrome

    OpenAIRE

    Shiefa, S.; Amargandhi, M.; Bhupendra, J.; Moulali, S.; Kristine, T.

    2012-01-01

    The first trimester screening programme offers a noninvasive option for the early detection of aneuploidy pregnancies. This screening is done by a combination of two biochemical markers i.e. serum free β-human chorionic gonadotrophin (free β-hCG) and pregnancy associated plasma protein A (PAPP-A), maternal age and fetal nuchal translucency (NT) thickness at 11 + 0–13 + 6 weeks of gestation. A beneficial consequence of screening is the early diagnosis or trisomies 21, 18 and 13. At 11 + 0–13 +...

  20. Bruk av amniocenteser og chorionbiopsier i Norge

    Directory of Open Access Journals (Sweden)

    Guttorm Haugen

    2009-10-01

    Full Text Available  SAMMENDRAGInvasiv prenatal diagnostikk i form av amniocentese (fostervannsprøve og chorionbiopsi (morkakeprøveutføres i ca. 2% av alle svangerskap i Norge per år. Dette er betydelig færre undersøkelser ennhva som utføres i de andre nordiske land. De fleste får utført amniocentese pga. høy maternell alder(aldersindikasjon som her i landet er ≥ 38 år ved fødselstermin. Chorionbiopsi er forbeholdt kvinnermed kjente arvelige lidelser i familien, dvs. kvinner med høy risiko for å få et affisert foster. De undersøkelsersom foreligger over svangerskapsutfall samt forekomst av komplikasjoner etter amniocenteseog chorionbiopsi er hovedsakelig utført i andre land på kvinner med generelt lavere risiko ( ≥ 35 år ennfor dem som får utført invasiv prenatal diagnostikk i Norge. Pga. vår restriktive praksis kan ikke disseresultatene uten videre overføres til Norge. Vi mangler eksakte data over svangerskapsutfall og evt.komplikasjoner etter disse undersøkelsene i en norsk populasjon.Haugen G, van der Hagen CB. The use of amniocentesis and chorionic villus sampling in Norway.Nor J Epidemiol ENGLISH SUMMARYAmniocentesis or chorionic villus sampling are performed in about 2% of all pregnancies in Norwaywhich is far less than in the other Nordic countries. Most of the amniocenteses are performed due toadvanced maternal age. In Norway this is defined as maternal age ≥ 38 years at term. Couples withknown chromosomal aberrations or genetic diseases in their families, i.e. women at a high risk of havingan affected fetus, are offered chorionic villus sampling. Earlier studies on complications and pregnancyoutcome following amniocentesis or chorionic villus sampling have been performed in other countriesmainly on women at a lower risk ( ≥ 35 years than for the women having such tests in Norway. We donot have data on pregnancy outcome and possible complications following amniocentesis and chorionicvillus sampling in a Norwegian

  1. Pro-inflammatory profile of preeclamptic placental mesenchymal stromal cells: new insights into the etiopathogenesis of preeclampsia.

    Directory of Open Access Journals (Sweden)

    Alessandro Rolfo

    Full Text Available UNLABELLED: The objective of the present study was to evaluate whether placental mesenchymal stromal cells (PDMSCs derived from normal and preeclamptic (PE chorionic villous tissue presented differences in their cytokines expression profiles. Moreover, we investigated the effects of conditioned media from normal and PE-PDMSCs on the expression of pro-inflammatory Macrophage migration Inhibitory Factor (MIF, Vascular Endothelial Growth Factor (VEGF, soluble FMS-like tyrosine kinase-1 (sFlt-1 and free β-human Chorionic Gonadotropin (βhCG by normal term villous explants. This information will help to understand whether anomalies in PE-PDMSCs could cause or contribute to the anomalies typical of preeclampsia. METHODS: Chorionic villous PDMSCs were isolated from severe preeclamptic (n = 12 and physiological control term (n = 12 placentae. Control and PE-PDMSCs's cytokines expression profiles were determined by Cytokine Array. Control and PE-PDMSCs were plated for 72 h and conditioned media (CM was collected. Physiological villous explants (n = 48 were treated with control or PE-PDMSCs CM for 72 h and processed for mRNA and protein isolation. MIF, VEGF and sFlt-1 mRNA and protein expression were analyzed by Real Time PCR and Western Blot respectively. Free βhCG was assessed by immunofluorescent. RESULTS: Cytokine array showed increased release of pro-inflammatory cytokines by PE relative to control PDMSCs. Physiological explants treated with PE-PDMSCs CM showed significantly increased MIF and sFlt-1 expression relative to untreated and control PDMSCs CM explants. Interestingly, both control and PE-PDMSCs media induced VEGF mRNA increase while only normal PDMSCs media promoted VEGF protein accumulation. PE-PDMSCs CM explants released significantly increased amounts of free βhCG relative to normal PDMSCs CM ones. CONCLUSIONS: Herein, we reported elevated production of pro-inflammatory cytokines by PE-PDMSCs. Importantly, PE PDMSCs

  2. Urinary gonadotrophin peptide--isolation and purification, and its immunohistochemical distribution in normal and neoplastic tissues.

    OpenAIRE

    Kardana, A.; M. E. Taylor; Southall, P. J.; Boxer, G. M.; Rowan, A. J.; Bagshawe, K. D.

    1988-01-01

    A urinary gonadotrophin peptide (UGP) was isolated and purified from semi-purified human chorionic gonadotrophin (hCG), prepared from pregnancy urine. The peptide showed hCG-B subunit activity and no hCG-alpha subunit activity as demonstrated by binding studies with the relevant antibodies. It had a molecular weight significantly less than hCG-B subunit. The peptide was linked to thyroglobulin and this conjugate used to immunise rabbits and mice. A radioimmunoassay (RIA) using 125I-UGP and th...

  3. Heterophile Antibody Interference led to Unneeded Chemotherapy in a Testicular Cancer Patient.

    Science.gov (United States)

    Soares, Daniele G; Millot, Françoise; Lacroix, Isabelle; Lotz, Jean-Pierre

    2016-11-01

    Human heterophile antibodies may develop after infection or contact with animal tissues or animal serum products. These antibodies have the capacity to bind to the animal immunoglobulins used in immunoassays leading to erroneous results. We here report a case of a testicular germ cell tumor patient who developed heterophile antibodies during the surveillance period of his disease. Following false-positive results of human chorionic gonadotropin (hCG) he received unneeded chemotherapy. This article also stresses the problem of using serum tumor markers without no major imaging abnormalities to diagnose a patients' relapse. PMID:27617210

  4. Heterophilic antibodies interfering with radioimmunoassay. A false-positive pregnancy test

    International Nuclear Information System (INIS)

    A young woman with amenorrhea had a consistently positive pregnancy test result (serum radioimmunoassay measurement of #betta#-human chorionic gonadotropin hormone). No fetal or placental tissue was found after uterine curettage and exploratory laparotomy. The false-positive pregnancy test result was due to heterophilic antibovine and antigoat antibodies in the patient's serum. These antibodies interfered with radioimmunoassays using goat antibodies. This case shows that serum heterophilic antibodies can interfere with immunoassays and result in unnecessary diagnostic procedures and/or unnecessary treatment

  5. A Resource for the Transcriptional Signature of Bona Fide Trophoblast Stem Cells and Analysis of Their Embryonic Persistence

    Directory of Open Access Journals (Sweden)

    Georg Kuales

    2015-01-01

    Full Text Available Trophoblast stem cells (TSCs represent the multipotent progenitors that give rise to the different cells of the embryonic portion of the placenta. Here, we analysed the expression of key TSC transcription factors Cdx2, Eomes, and Elf5 in the early developing placenta of mouse embryos and in cultured TSCs and reveal surprising heterogeneity in protein levels. We analysed persistence of TSCs in the early placenta and find that TSCs remain in the chorionic hinge until E9.5 and are lost shortly afterwards. To define the transcriptional signature of bona fide TSCs, we used inducible gain- and loss-of-function alleles of Eomes or Cdx2, and EomesGFP, to manipulate and monitor the core maintenance factors of TSCs, followed by genome-wide expression profiling. Combinatorial analysis of resulting expression profiles allowed for defining novel TSC marker genes that might functionally contribute to the maintenance of the TSC state. Analyses by qRT-PCR and in situ hybridisation validated novel TSC- and chorion-specific marker genes, such as Bok/Mtd, Cldn26, Duox2, Duoxa2, Nr0b1, and Sox21. Thus, these expression data provide a valuable resource for the transcriptional signature of bona fide and early differentiating TSCs and may contribute to an increased understanding of the transcriptional circuitries that maintain and/or establish stemness of TSCs.

  6. Phenylalanine metabolism regulates reproduction and parasite melanization in the malaria mosquito.

    Science.gov (United States)

    Fuchs, Silke; Behrends, Volker; Bundy, Jacob G; Crisanti, Andrea; Nolan, Tony

    2014-01-01

    The blood meal of the female malaria mosquito is a pre-requisite to egg production and also represents the transmission route for the malaria parasite. The proper and rapid assimilation of proteins and nutrients in the blood meal creates a significant metabolic challenge for the mosquito. To better understand this process we generated a global profile of metabolite changes in response to blood meal of Anopheles gambiae, using Gas Chromatography-Mass Spectrometry (GC-MS). To disrupt a key pathway of amino acid metabolism we silenced the gene phenylalanine hydroxylase (PAH) involved in the conversion of the amino acid phenylalanine into tyrosine. We observed increased levels of phenylalanine and the potentially toxic metabolites phenylpyruvate and phenyllactate as well as a reduction in the amount of tyrosine available for melanin synthesis. This in turn resulted in a significant impairment of the melanotic encapsulation response against the rodent malaria parasite Plasmodium berghei. Furthermore silencing of PAH resulted in a significant impairment of mosquito fertility associated with reduction of laid eggs, retarded vitellogenesis and impaired melanisation of the chorion. Carbidopa, an inhibitor of the downstream enzyme DOPA decarboxylase that coverts DOPA into dopamine, produced similar effects on egg melanization and hatching rate suggesting that egg chorion maturation is mainly regulated via dopamine. This study sheds new light on the role of amino acid metabolism in regulating reproduction and immunity.

  7. Bisphenol A Alters β-hCG and MIF Release by Human Placenta: An In Vitro Study to Understand the Role of Endometrial Cells

    Directory of Open Access Journals (Sweden)

    C. Mannelli

    2014-01-01

    Full Text Available A proper fetomaternal immune-endocrine cross-talk in pregnancy is fundamental for reproductive success. This might be unbalanced by exposure to environmental chemicals, such as bisphenol A (BPA. As fetoplacental contamination with BPA originates from the maternal compartment, this study investigated the role of the endometrium in BPA effects on the placenta. To this end, in vitro decidualized stromal cells were exposed to BPA 1 nM, and their conditioned medium (diluted 1 : 2 was used on chorionic villous explants from human placenta. Parallel cultures of placental explants were directly exposed to 0.5 nM BPA while, control cultures were exposed to the vehicle (EtOH 0.1%. After 24–48 h, culture medium from BPA-treated and control cultures was assayed for concentration of hormone human Chorionic Gonadotropin (β-hCG and cytokine Macrophage Migration Inhibitory Factor (MIF. The results showed that direct exposure to BPA stimulated the release of both MIF and β-hCG. These effects were abolished/diminished in placental cultures exposed to endometrial cell-conditioned medium. GM-MS analysis revealed that endometrial cells retain BPA, thus reducing the availability of this chemical for the placenta. The data obtained highlight the importance of in vitro models including the maternal component in reproducing the effects of environmental chemicals on human fetus/placenta.

  8. Localization and possible role of membrane type metallo-proteinase and tissue inhibitors of metalloproteinase-1 in early stages of placentation

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Human placental tissues from the first and second trimesters of gestation have been investigated using riboprobe in situ hybridisation of mRNA sequences coding for membrane type metalloproteinase (MT-1-MMP) and tissue inhibitors of metalloproteinase-1 (TIMP-1). Results show that (i) both mRNAs express at a relatively high level in the chorion laeve trophoblast cells and the adjacent decidual cells of fetal membrane; (ii) the most abundant expression of the two mRNAs was found in the extravillous trophoblast between Rohrs and Nitabuch striae of basal plate, trophoblast shell and gland cells of the decidua; (iii) isolated or small groups of cytotrophoblast cells in the chorionic villi and in the cells lining arterioles in decidua and stem villi also expressed both MT-1-MMP and TIMP-1 at defferent extents. The data suggest that the coordinated expression of the MT-MMP and its inhibitor TIMP in defferent cells of the placental tissue may play an essential role in trophoblast invasion and angiogenesis related to placentation in the first two trimesters of gestation. They may also have an ability to effect separation of fetal from material tissue at a favorable junctional site during parturition.

  9. Prenatal diagnosis of Crigler-Najjar syndrome type I by single-strand conformation polymorphism (SSCP).

    Science.gov (United States)

    Francoual, Jeanne; Trioche, Pascale; Mokrani, Chahnez; Seboui, Hassen; Khrouf, Naïma; Chalas, Jacqueline; Clement, Marina; Capel, Liliane; Tachdjian, Gérard; Labrune, Philippe

    2002-10-01

    Crigler-Najjar syndrome type I (CN-I) is a rare and severe inherited disorder of bilirubin metabolism, caused by the total deficiency of bilirubin-UDP-glucuronosyltransferase (UGT) activity. Enzymatic diagnosis cannot be performed in chorionic villi or amniocytes as UGT is not active in these tissues. The cloning of the UGT1 gene and the identification of disease-causing mutations have led to the possibility of performing DNA-based diagnosis. Here we report DNA-based prenatal diagnosis of CN-I in two Tunisian families in whom CN-I patients were diagnosed. As we had previously shown that CN-I was, in Tunisia, associated with homozygosity for the Q357R mutation within the UGT1 gene, we were able to detect this mutation in both families and to show that it was easily recognized by single-strand conformation polymorphism (SSCP) analysis. In both cases, SSCP analysis of fetal DNA showed that the fetus was heterozygous for the Q357R mutation. In one family, the pregnancy was carried to term and a healthy baby was born, whereas, in the other family, the pregnancy is still continuing. Thus the prenatal diagnosis of CN-I is possible, provided disease-causing mutations have been identified. SSCP analysis of DNA prepared either from amniocytes or from chorionic villi is a simple, reliable and fast method for prenatal diagnosis.

  10. Production of an acellular matrix from amniotic membrane for the synthesis of a human skin equivalent.

    Science.gov (United States)

    Sanluis-Verdes, Anahí; Yebra-Pimentel Vilar, Maria Teresa; García-Barreiro, Juan Javier; García-Camba, Marta; Ibáñez, Jacinto Sánchez; Doménech, Nieves; Rendal-Vázquez, Maria Esther

    2015-09-01

    Human amniotic membrane (HAM) has useful properties as a dermal matrix substitute. The objective of our work was to obtain, using different enzymatic or chemical treatments to eliminate cells, a scaffold of acellular HAM for later use as a support for the development of a skin equivalent. The HAM was separated from the chorion, incubated and cryopreserved. The membrane underwent different enzymatic and chemical treatments to eliminate the cells. Fibroblasts and keratinocytes were separately obtained from skin biopsies of patients following a sequential double digestion with first collagenase and then trypsin-EDTA (T/E). A skin equivalent was then constructed by seeding keratinocytes on the epithelial side and fibroblasts on the chorionic side of the decellularizated HAM. Histological, immunohistochemical, inmunofluorescent and molecular biology studies were performed. Treatment with 1% T/E at 37 °C for 30 min totally removed epithelial and mesenchymal cells. The HAM thus treated proved to be a good matrix to support adherence of cells and allowed the achievement of an integral and intact scaffold for development of a skin equivalent, which could be useful as a skin substitute for clinical use.

  11. Empty follicle syndrome-Still an enigma

    Directory of Open Access Journals (Sweden)

    Deepika Krishna

    2008-01-01

    Full Text Available Empty follicle syndrome (EFS, although rare with an incidence of 0.2-7%, is a frustrating condition where no oocytes are retrieved in in vitro fertilization (IVF, even though ultrasound and estradiol measurements show the presence of many potential follicles. It is a complex phenomenon that cannot be explained by low bioavailability of human chorionic gonadotrophin alone; neither can it be reliably diagnosed by the measurement of serum beta-human chorionic gonadotrophin (bhCG on the day of oocyte retrieval (OR, except possibly when the bhCG concentration is very low. Here we report a case who underwent intracytoplasmic sperm injection (ICSI for her partner′s severe oligoasthenozoospermia. Controlled ovarian hyperstimulation (COH was done in her first cycle of ICSI, using a gonadotrophin-releasing hormone (GnRH agonist long protocol with follicle-stimulating hormone (FSH and human menopausal gonadotrophin (HMG. However, as we were unable to retrieve any oocytes, her COH protocol was changed in the subsequent cycle with a successful outcome.

  12. Tissue-specific Differentiation Potency of Mesenchymal Stromal Cells from Perinatal Tissues.

    Science.gov (United States)

    Kwon, Ahlm; Kim, Yonggoo; Kim, Myungshin; Kim, Jiyeon; Choi, Hayoung; Jekarl, Dong Wook; Lee, Seungok; Kim, Jung Min; Shin, Jong-Chul; Park, In Yang

    2016-04-05

    Human perinatal tissue is an abundant source of mesenchymal stromal cells(MSCs) and lacks the ethical concerns. Perinatal MSCs can be obtained from various tissues as like amnion, chorion, and umbilical cord. Still, little is known of the distinct nature of each MSC type. In this study, we successfully isolated and cultured MSCs from amnion(AMSCs), chorion(CMSCs), and umbilical cord(UC-MSCs). Proliferation potential was different among them, that AMSCs revealed the lowest proliferation rate due to increased Annexin V and senescence-associated β-galactosidase positive cells. We demonstrated distinct characteristic gene expression according to the source of the original tissue using microarray. In particular, genes associated with apoptosis and senescence including CDKN2A were up-regulated in AMSCs. In CMSCs, genes associated with heart morphogenesis and blood circulation including HTR2B were up-regulated. Genes associated with neurological system processes including NPY were up-regulated in UC-MSCs. Quantitative RT-PCR confirmed the gene expression data. And in vitro differentiation of MSCs demonstrated that CMSCs and UC-MSCs had a more pronounced ability to differentiate into cardiomyocyte and neural cells, respectively. This study firstly demonstrated the innate tissue-specific differentiation potency of perinatal MSCs which can be helpful in choosing more adequate cell sources for better outcome in a specific disease.

  13. Triple test role in identifying chromosomal disorders in the second trimester of pregnancy

    Directory of Open Access Journals (Sweden)

    Artenie Vlad

    2011-06-01

    Full Text Available The triple test plays a very important role in identifying chromosomal disorders, in the prenatal screening of the second pregnancy trimester. The scope of our research resides in investigating the level of human chorionic gonadotropin, alpha-fetoprotein and unconjugated estriol (markers that make-up the triple test, in the serum sampled and analysed from a group of 135 pregnant women. The observation of the above mentioned markers is made in order to identify the pregnancies that present a higher risk for the appearance of chromosomal disorders. We also, decided to associate the values gathered for human chorionic gonadotropin, alpha-fetoprotein and unconjugated estriol, with the maternal age. The interpretation of the data was made using the PRISCA 4.0 software, considering by default the gestational age, smoking, in vitro fertilization, diabetic status, medical history of the mother. We must say that the patients were pregnant in the second trimester, period specific for triple test survey and are not the same patients included in the double test survey. Following the conducted biochemical analyses normal values were obtained, values that fit the ranges specified in the specific literature, but also values that were outside the normal ranges, indentifying in this way pregnancies with high risk for 21 and 18 trisomy.

  14. Effect of Culture Medium Supplementation with b-mercaptoethanol and Amino Acid on Canine Intergeneric Embryo Development with Porcine Oocyte Cytoplasm Recipient

    Directory of Open Access Journals (Sweden)

    Yuda Heru Fibrianto

    2015-11-01

    Full Text Available The present study investigated the effect of culture medium supplementation with mercaptoethanol ( MEand amino acid (AA on canine intergeneric embryo development with porcine oocyte cytoplasm. Porcine cumulusoocyte complexes (COCs were collected from slaughterhouse and matured in TCM-199 supplemented with 26.2mM NaHCO, 3.05 mM glucose, 0.91 mM sodium pyruvate, 0.57 mM L-cysteine, 75 mg/l kanamycin, 10 ng/ml 3epidermal growth factor, equine chorionic gonadotropin (eCG, 10 IU/ml human chorionic gonadotropin (hCG,and 10% (v/v porcine follicular fluid (pFF at 39 °C in a humidified atmosphere of 5% CO for 42-44 h and donor cell 2collected from ear skin afghanhound male dog. After somatic cell nuclear transfer (SCNT, embryo developmentwere examined for cleavage rate and 144 hr for final development after cultured in media. The result shows that,amino acid and mercapoethanol addition in culture medium (NCSU-23 have no effect on embryo development.The development rate of embryo until 16 cell stage in NCSU and NCSU supplement are 4.67% and morula stage are3.73% and 4.67%.Key words : intergeneric clone embryo, canine, ( amino acid (AA

  15. DNA immunization with plasmids expressing hCGbeta-chimeras.

    Science.gov (United States)

    Terrazzini, Nadia; Hannesdóttir, Sólveig; Delves, Peter J; Lund, Torben

    2004-06-01

    Human chorionic gonadotropin has been used as an anti-fertility vaccine and as a target for cancer immunotherapy. We have explored the use of DNA immunization with the aim of improving the immunogenicity of this hormone. Stimulating the muscle with electric pulses following intramuscular injection of plasmids expressing hCGbeta resulted in higher levels of human chorionic gonadotropin (hCG)-specific antibodies, which could be further enhanced following a protein boost with hCG mixed with adjuvant. DNA vaccines encoding a membrane attached or a secreted form of hCGbeta produced similar-albeit relatively modest-antibody responses. Providing hCGbeta with additional T cell help by vaccinating with a plasmid encoding a hCGbeta-hFc fusion protein did not further increase the antibody levels in the immunized animals. However, immunization of mice with a construct encoding hCGbeta fused to C3d(3) produced significantly lower antibody levels relative to mice immunized with the hCGbeta-alone expression plasmid, even though the hCGbeta-C3d(3) chimera was expected to facilitate cross-linking of the antigen-specific B-cell receptor and CR2 thereby lowering the threshold of activation. Thus the limiting factor determining the antibody levels following hCGbeta immunization, at least for DNA immunization, is related to the amount of protein available rather than the form of protein produced or lack of T cell epitopes. PMID:15149771

  16. Assessment of fetal cell chimerism in transgenic pig lines generated by Sleeping beauty transposition.

    Science.gov (United States)

    Garrels, Wiebke; Holler, Stephanie; Taylor, Ulrike; Herrmann, Doris; Niemann, Heiner; Ivics, Zoltan; Kues, Wilfried A

    2014-01-01

    Human cells migrate between mother and fetus during pregnancy and persist in the respective host for long-term after birth. Fetal microchimerism occurs also in twins sharing a common placenta or chorion. Whether microchimerism occurs in multiparous mammals such as the domestic pig, where fetuses have separate placentas and chorions, is not well understood. Here, we assessed cell chimerism in litters of wild-type sows inseminated with semen of transposon transgenic boars. Segregation of three independent monomeric transposons ensured an excess of transgenic over non-transgenic offspring in every litter. Transgenic siblings (n = 35) showed robust ubiquitous expression of the reporter transposon encoding a fluorescent protein, and provided an unique resource to assess a potential cell trafficking to non-transgenic littermates (n = 7) or mothers (n = 4). Sensitive flow cytometry, fluorescence microscopy, and real-time PCR provided no evidence for microchimerism in porcine littermates, or piglets and their mothers in both blood and solid organs. These data indicate that the epitheliochorial structure of the porcine placenta effectively prevents cellular exchange during gestation. PMID:24811124

  17. Increased nuchal translucency and diaphragmatic hernia. A case report.

    Science.gov (United States)

    Daniilidis, A; Balaouras, D; Psarra, N; Chitzios, D; Tzafettas, M; Balaouras, G; Vrachnis, N

    2015-01-01

    Increased nuchal translucency (NT) thickness is present in 40% of fetuses with diaphragmatic hernia, including 80% of those that result in neonatal death and in 20% of the survivors. A 33-year-old nulliparous woman had first trimester scan at 12 weeks. The fetus had a NT of 2.3 mm, normal ductus venosus (DV), and tricuspid doppler and present nasal bone. Pregnancy-associated plasma protein A (PAPP-A) was 0.59 MoM and beta-human chorionic gonadotropin (b-hCG) 2.56 MoM. The couple did not opt for chorionic villous sampling (CVS) and repeat ultrasound examination was advised. At 18 weeks, ultrasound revealed left sided diaphragmatic hernia. The couple consented for termination of the pregnancy. The molecular test showed normal karyotype and male gender. In such cases with intrathoracic herniation of abdominal viscera, the increased NT may be the consequence of venous congestion due to mediastinal compression. The prolonged compression of the lungs causes pulmonary hypoplasia. Increased NT with normal fetal karyotype is associated with structural fetal anomalies like diaphragmatic hernia and screening at 16-18 weeks is imperative. PMID:26054128

  18. Expression of natural antimicrobials by human placenta and fetal membranes.

    Science.gov (United States)

    King, A E; Paltoo, A; Kelly, R W; Sallenave, J-M; Bocking, A D; Challis, J R G

    2007-01-01

    Preterm birth associated with infection is a major clinical problem. We hypothesized that this condition is associated with altered expression of natural antimicrobial molecules (beta-defensins (HBD), elafin). Therefore, we examined expression of these molecules and their regulation by proinflammatory cytokines in placentae and fetal membranes from term pregnancy. HBD1-3 and elafin were localized by immunohistochemistry in fetal membranes and placenta. Real-time quantitative PCR was used to examine mRNA expression in primary trophoblast cells treated with inflammatory molecules. HBD1-3 and elafin were immunolocalized to placental and chorion trophoblast layers of fetal membranes and placenta. Immunoreactivity was also observed in amnion epithelium and decidua. No differences were noted between samples from women who were not in labour compared to those in active labour. In in vitro cultures of primary trophoblast cells, HBD2 and elafin mRNA expression was upregulated by the proinflammatory cytokine, IL-1beta. These results suggest that the chorion and placental trophoblast layers may be key barriers to the progression of infection in the pregnant uterus. Natural antimicrobial expression may be altered in response to inflammatory mediator expression associated with the onset of labour and/or uterine infection, providing increased protection when the uterus may be particularly susceptible to infection. PMID:16513165

  19. Effects of diets supplemented by fish oil on sex ratio of pups in bitch.

    Science.gov (United States)

    Gharagozlou, Faramarz; Youssefi, Reza; Akbarinejad, Vahid

    2016-01-01

    The present study was conducted to evaluate the effect of fish oil supplementation prior to mating on secondary sex ratio of pups (the proportion of males at birth) in bitches. Sixty five bitches (German Shepherd, n = 35; Husky, n = 30) were enrolled in the study. Bitches (140-150 days post-estrus) were given 2% per dry matter intake palm oil and fish oil in the control (n = 33) and treatment (n = 32) groups, respectively. To induce estrus, bitches were received equine chorionic gonadotropin (eCG) administration (50 IU kg(-1)) 30 days after nutritional supplementation followed by human chorionic gonadotropin (hCG) administration (500 IU per dog) seven days later. Bitches were introduced to dogs of the same breed after hCG administration. The weight of bitches was increased over time (p 0.05). The mating rate, pregnancy rate and litter size were not influenced by treatment and breed. Secondary sex ratio was higher in the treatment (105/164; 64.00%) than in the control (68/147; 46.30%) group (p birth. In addition, it appears that there might be variation among dog breeds with regard to the sex ratio of offspring. PMID:27482354

  20. The fowl tick, Argas (Persicargas) persicus (Ixodoidea: Argasidae): description of the egg and redescription of the larva by scanning electron microscopy.

    Science.gov (United States)

    Montasser, Ashraf A

    2010-12-01

    This scanning electron microscopy study revealed that the egg of Argas persicus was covered with chorion which appeared as a wrinkled layer containing regions of three textures. The first had elevated parts of slightly rough surface. The second had irregular smooth elevations; each carried numerous parallel horizontal foldings with vertical ridges. The last region had rough surface with irregularly shaped projections. Following the removal of the chorion, shell was observed to have one polar micropyle and numerous slit like openings. Length (L), width (W) and L/W ratio of the egg were measured. Investigation of larvae revealed extensively folded integument of idiosoma and spherical or elongated tubercles on dorsal plate. Mouth enclosed between ventral hypostome and two dorsal chelicerae. Hypostome carried four longitudinal rows of conical denticles. Each chelicera was made up of two segments; the basal one appeared as a pocket for the distal one. Haller's organ consisted of an anterior pit containing seven sensilla and a posterior capsule with four apertures. Distribution of chemo- and mechano-sensilla on the body was examined. Measurements of whole body, idiosoma, dorsal plate, capitulum, hypostome, palp and different types of sensilla both on the body and Haller's organ are also presented. PMID:20607364

  1. Effective treatment for malignant mediastinal teratoma.

    Science.gov (United States)

    Parker, D; Holford, C P; Begent, R H; Newlands, E S; Rustin, G J; Makey, A R; Bagshawe, K D

    1983-12-01

    Primary malignant mediastinal teratoma is a rare tumour previously regarded as inevitably fatal. In a series of eight male patients with a mean age of 24 years five remain alive and well. All patients showed raised serum concentrations of human chorionic gonadotrophin or alpha fetoprotein. The patients were treated with intermittent combination chemotherapy that included cisplatin. Six patients responded to chemotherapy with a fall in human chorionic gonadotrophin or alpha fetoprotein to near normal levels and they then had radical excision of the remaining tumour. Living malignant tumour was found in four of the specimens and these patients received postoperative chemotherapy. One patient died after eight months and the remaining five patients are alive and well 13-136 months after the start of treatment. The two patients who did not undergo surgery died at one month and 15 months. Intermittent combination chemotherapy and carefully timed radical excision of these tumours would appear to have produced better results than have been reported in other series.

  2. Invasive implantation and intimate placental associations in a placentotrophic African lizard, Trachylepis ivensi (scincidae).

    Science.gov (United States)

    Blackburn, Daniel G; Flemming, Alexander F

    2012-02-01

    In the viviparous lizard Trachylepis ivensi (Scincidae) of central Africa, reproducing females ovulate tiny ∼1 mm eggs and supply the nutrients for development by placental means. Histological study shows that this species has evolved an extraordinary placental pattern long thought to be confined to mammals, in which fetal tissues invade the uterine lining to contact maternal blood vessels. The vestigial shell membrane disappears very early in development, allowing the egg to absorb uterine secretions. The yolk is enveloped precocially by the trilaminar yolk sac and no isolated yolk mass or yolk cleft develops. Early placentas are formed from the chorion and choriovitelline membranes during the neurula through pharyngula stages. During implantation, cells of the chorionic ectoderm penetrate between uterine epithelial cells. The penetrating tissue undergoes hypertrophy and hyperplasia, giving rise to sheets of epithelial tissue that invade beneath the uterine epithelium, stripping it away. As a result, fetal epithelium entirely replaces the uterine epithelium, and lies in direct contact with maternal capillaries and connective tissue. Placentation is endotheliochorial and fundamentally different from that of all other viviparous reptiles known. Further, the pattern of fetal membrane development (with successive loss and re-establishment of an extensive choriovitelline membrane) is unique among vertebrates. T. ivensi represents a new extreme in placental specializations of reptiles, and is the most striking case of convergence on the developmental features of viviparous mammals known. PMID:21956253

  3. Microarray-Based Analysis of Methylation Status of CpGs in Placental DNA and Maternal Blood DNA--Potential New Epigenetic Biomarkers for Cell Free Fetal DNA-Based Diagnosis.

    Directory of Open Access Journals (Sweden)

    Lotte Hatt

    Full Text Available Epigenetic markers for cell free fetal DNA in the maternal blood circulation are highly interesting in the field of non-invasive prenatal testing since such markers will offer a possibility to quantify the amount of fetal DNA derived from different chromosomes in a maternal blood sample. The aim of the present study was to define new fetal specific epigenetic markers present in placental DNA that can be utilized in non-invasive prenatal diagnosis. We have conducted a high-resolution methylation specific beadchip microarray study assessing more than 450.000 CpG sites. We have analyzed the DNA methylation profiles of 10 maternal blood samples and compared them to 12 1st trimesters chorionic samples from normal placentas, identifying a number of CpG sites that are differentially methylated in maternal blood cells compared to chorionic tissue. To strengthen the utility of these differentially methylated CpG sites to be used with methyl-sensitive restriction enzymes (MSRE in PCR-based NIPD, we furthermore refined the list of selected sites, containing a restriction sites for one of 16 different methylation-sensitive restriction enzymes. We present a list of markers on chromosomes 13, 18 and 21 with a potential for aneuploidy testing as well as a list of markers for regions harboring sub-microscopic deletion- or duplication syndromes.

  4. Decorin expression is decreased in first trimester placental tissue from pregnancies with small for gestation age infants at birth.

    Science.gov (United States)

    Murthi, P; van Zanten, D E; Eijsink, J J H; Borg, A J; Stevenson, J L; Kalionis, B; Chui, A K; Said, J M; Brennecke, S P; Erwich, J J H M

    2016-09-01

    Fetal growth restriction (FGR) is a leading cause of perinatal morbidity and mortality. FGR pregnancies are often associated with histological evidence of placental vascular thrombosis. The proteoglycans are important components and regulators of vascular homeostasis. Previous studies from our laboratory highlighted mRNA and protein expression differences in placental proteoglycan decorin (DCN), within a clinically well-characterised cohort of third-trimester idiopathic FGR compared with gestation-matched uncomplicated control pregnancies. We also showed that decorin contributes to abnormal angiogenesis and increased thrombin generation in vitro. These observations suggest that DCN gene expression may contribute to the etiology of FGR. Small for gestational age (SGA) is frequently used as a proxy for FGR and is defined as a birth weight below the 10th percentile of a birth weight curve. We therefore made use of a unique resource of first trimester tissues obtained via chorionic villus sampling during the first trimester to investigate the temporal relationship between altered DCN expression and any subsequent development of SGA. We hypothesized that placental DCN expression is decreased early in gestation in SGA pregnancies. Surplus chorionic villus specimens from 15 women subsequently diagnosed with FGR and 50 from women with uncomplicated pregnancies were collected. DCN mRNA and DCN protein were determined using real-time PCR and immunoblotting, respectively. Both DCN mRNA and protein were significantly decreased in placentae from first-trimester SGA-pregnancies compared with controls (p development of SGA. PMID:27577711

  5. Comparative radiochemical and radio-immunometrical study on trophoblast-dependent serum parameters under parturition, and radio-immunological detection of pregnancy-specific β1-glycoprotein [SP1] in early pregnancy

    International Nuclear Information System (INIS)

    77 deliveries were studied clinically and chemically bench-scale using data acquisition sheets applicable for computer operation. According to a catalog 30 normal, 32 abnormal and 15 births featuring EPH gestosis were clearly defined and classified. Radiochemical or radio-immunometrical methods were used to simultaneously analyse and evaluate statistically the following 'trophoblast-dependent serum parameters' in subpartem and postpartem maternal blood, and in aterialized or pooled venous-arterialized umbilicalchord blood in the case groups mentioned above: - 17 β-hydroxysteroid; NAD(P)-oxydoreductase (17 β-HSD; - free oestriol (fE3); -β-subunit of Human Chorionic Gonadotropin (β-hCG); - Human Chorionic Somatomammatropin (hCS=hPL); - and pregnancy-specific β1-glycoprotein (SP1). A systematic study was made for the statistical correlations between trophoblast - dependent serum parameters and plancental and neonatal findings as well as for possible correlations between the serum parameters in both the maternal and fetal blood distribution spaces. (orig./MG)

  6. Fetal growth disorders in twin gestations.

    LENUS (Irish Health Repository)

    Breathnach, Fionnuala M

    2012-06-01

    Twin growth is frequently mismatched. This review serves to explore the pathophysiologic mechanisms that underlie growth aberrations in twin gestations, the prenatal recognition of abnormal twin growth, and the critical importance of stratifying management of abnormal twin growth by chorionicity. Although poor in utero growth of both twins may reflect maternal factors resulting in global uteroplacental dysfunction, discordant twin growth may be attributed to differences in genetic potential between co-twins, placental dysfunction confined to one placenta only, or one placental territory within a shared placenta. In addition, twin-twin transfusion syndrome represents a distinct entity of which discordant growth is a common feature. Discordant growth is recognized as an independent risk factor for adverse perinatal outcome. Intertwin birth weight disparity of 18% or more should be considered to represent a discordance threshold, which serves as an independent risk factor for adverse perinatal outcome. At this cutoff, perinatal morbidity is found to increase both for the larger and the smaller twin within a discordant pair. There remains uncertainty surrounding the sonographic parameters that are most predictive of discordance. Although heightening of fetal surveillance in the face of discordant twin growth follows the principles applied to singleton gestations complicated by fetal growth restriction, the timing of intervention is largely influenced by chorionicity.

  7. Phenylalanine metabolism regulates reproduction and parasite melanization in the malaria mosquito.

    Directory of Open Access Journals (Sweden)

    Silke Fuchs

    Full Text Available The blood meal of the female malaria mosquito is a pre-requisite to egg production and also represents the transmission route for the malaria parasite. The proper and rapid assimilation of proteins and nutrients in the blood meal creates a significant metabolic challenge for the mosquito. To better understand this process we generated a global profile of metabolite changes in response to blood meal of Anopheles gambiae, using Gas Chromatography-Mass Spectrometry (GC-MS. To disrupt a key pathway of amino acid metabolism we silenced the gene phenylalanine hydroxylase (PAH involved in the conversion of the amino acid phenylalanine into tyrosine. We observed increased levels of phenylalanine and the potentially toxic metabolites phenylpyruvate and phenyllactate as well as a reduction in the amount of tyrosine available for melanin synthesis. This in turn resulted in a significant impairment of the melanotic encapsulation response against the rodent malaria parasite Plasmodium berghei. Furthermore silencing of PAH resulted in a significant impairment of mosquito fertility associated with reduction of laid eggs, retarded vitellogenesis and impaired melanisation of the chorion. Carbidopa, an inhibitor of the downstream enzyme DOPA decarboxylase that coverts DOPA into dopamine, produced similar effects on egg melanization and hatching rate suggesting that egg chorion maturation is mainly regulated via dopamine. This study sheds new light on the role of amino acid metabolism in regulating reproduction and immunity.

  8. Study of Positive and Negative Consequences of Using GnRH Antagonist in Intrauterine Insemination Cycles

    Directory of Open Access Journals (Sweden)

    Maryam Bagheri

    2009-01-01

    Full Text Available Background: To assess the usefulness of premature luteinization hormone (LH surge preventionin an intrauterine insemination (IUI cycle by GnRH antagonist administration.Materials and Methods: Sixty patients with unexplained or mild male infertility or minimalto mild endometriosis were enrolled in this prospective randomized controlled trial. There weretwenty patients in group A (with GnRH antagonist and 40 patients in group B (without GnRHantagonist.In all of the participants, clomiphene citrate and human menopausal gonadotropin (CC+HMG wereused for ovarian stimulation. When at least one follicle with ≥ 16 mm diameter was seen, LH surgewas checked by a urinary LH kit. In patients with negative results, human chorionic gonadotropinwas continued in both groups, but in group A 0.25 mg Ganirelix SQ was administered for two days,,then in both groups human chorionic gonadotropin (HCG was injected on the third day and IUIwas done 36-40 hours later. Ongoing pregnancy was the primary outcome.Results: Baseline characters and clinical parameters were similar in both groups with the exceptionof ≥14 mm follicles which were higher in group A (p value= 0.003. The pregnancy rate in bothgroups was not significantly different, although it was higher in group B (10% in group A and 15%in group B.Conclusion: At least in CC+HMG stimulated cycles for IUI, the occurrence of premature LHsurge could have a useful rule and GnRH antagonist administration could be an inappropriateintervention.

  9. Bisphenol A alters β-hCG and MIF release by human placenta: an in vitro study to understand the role of endometrial cells.

    Science.gov (United States)

    Mannelli, C; Ietta, F; Carotenuto, C; Romagnoli, R; Szostek, A Z; Wasniewski, T; Skarzynski, D J; Paulesu, Luana

    2014-01-01

    A proper fetomaternal immune-endocrine cross-talk in pregnancy is fundamental for reproductive success. This might be unbalanced by exposure to environmental chemicals, such as bisphenol A (BPA). As fetoplacental contamination with BPA originates from the maternal compartment, this study investigated the role of the endometrium in BPA effects on the placenta. To this end, in vitro decidualized stromal cells were exposed to BPA 1 nM, and their conditioned medium (diluted 1 : 2) was used on chorionic villous explants from human placenta. Parallel cultures of placental explants were directly exposed to 0.5 nM BPA while, control cultures were exposed to the vehicle (EtOH 0.1%). After 24-48 h, culture medium from BPA-treated and control cultures was assayed for concentration of hormone human Chorionic Gonadotropin ( β -hCG) and cytokine Macrophage Migration Inhibitory Factor (MIF). The results showed that direct exposure to BPA stimulated the release of both MIF and β -hCG. These effects were abolished/diminished in placental cultures exposed to endometrial cell-conditioned medium. GM-MS analysis revealed that endometrial cells retain BPA, thus reducing the availability of this chemical for the placenta. The data obtained highlight the importance of in vitro models including the maternal component in reproducing the effects of environmental chemicals on human fetus/placenta. PMID:24737926

  10. Alcohol consumption at the time of conception and spontaneous abortion.

    Science.gov (United States)

    Henriksen, Tine Brink; Hjollund, Niels Henrik; Jensen, Tina Kold; Bonde, Jens Peter; Andersson, Anna-Maria; Kolstad, Henrik; Ernst, Erik; Giwercman, Aleksander; Skakkebaek, Niels Erik; Olsen, Jørn

    2004-10-01

    The authors studied the association between female and male alcohol intakes at the time of conception and the risk of spontaneous abortion, including early pregnancy loss detected by urinary human chorionic gonadotropin. After a nationwide mailing to about 50,000 members of four trade unions in Denmark in 1992-1994, 430 couples without previous pregnancy attempts were enrolled when birth control was discontinued, and they were followed until a clinically recognized pregnancy or for six menstrual cycles. Alcohol intake and potential confounding factors were reported in monthly questionnaires. Women collected morning urine for 10 days from the first day of vaginal bleeding in each cycle. The authors detected 186 pregnancies: 131 resulted in childbirth, and 55 resulted in spontaneous abortion (34 detected by urinary human chorionic gonadotropin). Depending on the intake in the cycle of conception and the adjustment factors, female alcohol intake was associated with 2-3 times the adjusted risk of spontaneous abortion compared with no intake, and male alcohol intake was associated with 2-5 times the adjusted risk. Only the adjusted relative risks for 10 or more drinks/week compared with no intake were statistically significant. Both male and female alcohol intakes during the week of conception increased the risk of early pregnancy loss. PMID:15383410

  11. Acute chorioamnionitis and funisitis: definition, pathologic features, and clinical significance.

    Science.gov (United States)

    Kim, Chong Jai; Romero, Roberto; Chaemsaithong, Piya; Chaiyasit, Noppadol; Yoon, Bo Hyun; Kim, Yeon Mee

    2015-10-01

    Acute inflammatory lesions of the placenta consist of diffuse infiltration of neutrophils at different sites in the organ. These lesions include acute chorioamnionitis, funisitis, and chorionic vasculitis and represent a host response (maternal or fetal) to a chemotactic gradient in the amniotic cavity. While acute chorioamnionitis is evidence of a maternal host response, funisitis and chorionic vasculitis represent fetal inflammatory responses. Intraamniotic infection generally has been considered to be the cause of acute chorioamnionitis and funisitis; however, recent evidence indicates that "sterile" intraamniotic inflammation, which occurs in the absence of demonstrable microorganisms induced by "danger signals," is frequently associated with these lesions. In the context of intraamniotic infection, chemokines (such as interleukin-8 and granulocyte chemotactic protein) establish a gradient that favors the migration of neutrophils from the maternal or fetal circulation into the chorioamniotic membranes or umbilical cord, respectively. Danger signals that are released during the course of cellular stress or cell death can also induce the release of neutrophil chemokines. The prevalence of chorioamnionitis is a function of gestational age at birth, and present in 3-5% of term placentas and in 94% of placentas delivered at 21-24 weeks of gestation. The frequency is higher in patients with spontaneous labor, preterm labor, clinical chorioamnionitis (preterm or term), or ruptured membranes. Funisitis and chorionic vasculitis are the hallmarks of the fetal inflammatory response syndrome, a condition characterized by an elevation in the fetal plasma concentration of interleukin-6, and associated with the impending onset of preterm labor, a higher rate of neonatal morbidity (after adjustment for gestational age), and multiorgan fetal involvement. This syndrome is the counterpart of the systemic inflammatory response syndrome in adults: a risk factor for short- and long

  12. Antenatal cytogenetic testing in Havana, Cuba.

    Science.gov (United States)

    Méndez-Rosado, Luis A; Quiñones, Olga; Molina, Odalys; González, Nereida; del Sol, Marylin; Maceiras, Luanda; Bravo, Yomisleidy

    2014-01-01

    INTRODUCTION Antenatal cytogenetic testing was started in Havana in 1984, as a diagnostic option for fetal chromosome complement. The techniques applied are amniocyte culture, chorionic villus sampling, cordocentesis and fluorescence in situ hybridization in interphase cells. OBJECTIVE Describe the results of antenatal cytogenetic testing in the cytogenetic laboratory of the Cuba's National Medical Genetics Center in Havana, from 1984 through 2012. METHODS A retrospective descriptive study was carried out of the 22,928 pregnant women who had antenatal testing with conclusive results during the period 1984-2012. Information was obtained from laboratory databases for four antenatal diagnostic techniques. Variables studied were: antenatal diagnostic method, indications for genetic testing, type of chromosomal abnormality detected and couple's decision concerning pregnancy continuation if hereditary disease was diagnosed. Results were reported in absolute numbers and percentages. RESULTS Overall positivity was 2.8% (641 cases). Of the total, 20,565 samples were from amniocyte culture (558 positive cases, 2.7%); 1785 chorionic villus sampling (38 positive, 2.1%); 407 cord blood culture (28 positive, 6.9%); and 171 fluorescence in situ hybridization in interphase cells (17 positive, 9.9%). Advanced maternal age was the predominant indication for amniocyte culture and chorionic villus sampling. Positivity was higher for the two less frequently used methods, cordocentesis (6.9% positivity) and fluorescence in situ hybridization (9.9%). The predominant chromosomal abnormality was Down syndrome, with 45.4% of cases detected (291/641; 279 pure lines and 12 mosaic trisomies), followed by Edward syndrome with 12% (77/641, 71 pure lines and 6 mosaics) and Patau syndrome 4.7% (30/641, 27 pure lines and 3 mosaics). Sexual aneuploidy with pure lines affected 6.9% of cases (44/641) and with mosaicism 4.7% (30/641). Structural chromosomal abnormalities were detected in 22.5% of cases

  13. Ultrasensitive ELISA using enzyme-loaded nanospherical brushes as labels.

    Science.gov (United States)

    Qu, Zhenyuan; Xu, Hong; Xu, Ping; Chen, Kaimin; Mu, Rong; Fu, Jianping; Gu, Hongchen

    2014-10-01

    Improving the detection sensitivity of enzyme-linked immunosorbent assay (ELISA) is of utmost importance for meeting the demand of early disease diagnosis. Herein we report an ultrasensitive ELISA system using horseradish peroxidase (HRP)-loaded nanospherical poly(acrylic acid) brushes (SPAABs) as labels. HRP was covalently immobilized in SPAABs with high capacity and activity via an efficient "chemical conjugation after electrostatic entrapment" (CCEE) process, thus endowing SPAABs with high amplification capability as labels. The periphery of SPAAB-HRP was further utilized to bind a layer of antibody with high density for efficient capture of analytes owing to the three-dimensional architecture of SPAABs. Using human chorionic gonadotrophin (hCG) as a model analyte, the SPAAB-amplified system drastically boosted the detection limit of ELISA to 0.012 mIU mL(-1), a 267-fold improvement as compared to conventional ELISA systems.

  14. Application of immunomagnetic particles to enzyme-linked immunosorbent assay (ELISA) for improvement of detection sensitivity of HCG.

    Science.gov (United States)

    Kuo, Hsiao-Ting; Yeh, Jay Z; Wu, Po-Hua; Jiang, Chii-Ming; Wu, Ming-Chang

    2012-01-01

    This investigation was aimed at using superparamagnetic particles to enzyme-linked immunosorbent assay (SPIO-ELISA) of human chorionic gonadotropin (hCG) to enhance detection sensitivity of hCG. We found that N-(3-dimethyl aminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) was the best cross-linking reagent to link anti hCG α antibody to superparamagnetic particle (SPIO-anti hCG α antibody immunomagnetic particle). To improve the specificity of the assay, a horse radish peroxidase (HRP)-labeled anti-hCG beta monoclonal antibody was used to detect captured hCG using double antibody sandwich ELISA assay. SPIO-ELISA application to determine hCG increased the sensitivity to 1 mIU/mL, which is a level of sensitivity enabling the diagnosis of pregnancy during the early gestational period.

  15. The science behind 25 years of ovarian stimulation for in vitro fertilization.

    Science.gov (United States)

    Macklon, Nick S; Stouffer, Richard L; Giudice, Linda C; Fauser, Bart C J M

    2006-04-01

    To allow selection of embryos for transfer after in vitro fertilization, ovarian stimulation is usually carried out with exogenous gonadotropins. To compensate for changes induced by stimulation, GnRH analog cotreatment, oral contraceptive pretreatment, late follicular phase human chorionic gonadotropin, and luteal phase progesterone supplementation are usually added. These approaches render ovarian stimulation complex and costly. The stimulation of multiple follicular development disrupts the physiology of follicular development, with consequences for the oocyte, embryo, and endometrium. In recent years, recombinant gonadotropin preparations have become available, and novel stimulation protocols with less detrimental effects have been developed. In this article, the scientific background to current approaches to ovarian stimulation for in vitro fertilization is reviewed. After a brief discussion of the relevant aspect of ovarian physiology, the development, application, and consequences of ovarian stimulation strategies are reviewed in detail.

  16. Salvage therapy in patients with germ cell tumors.

    Science.gov (United States)

    Einhorn, Lawrence H

    2015-01-01

    Testicular cancer is the most curable metastatic solid tumor. Initial chemotherapy is evidence based with risk stratification into three prognostic categories: good, intermediate, and advanced disease. Guidelines for disease management following progression after initial cisplatin combination chemotherapy are less clear. Options include salvage surgery for patients with anatomically confined relapse, standard-dose cisplatin combination chemotherapy, or high-dose chemotherapy with carboplatin plus etoposide with peripheral blood stem cell transplantation. Proper interpretation of a presumed relapse can be complicated. Growing masses on imaging studies might reflect a growing teratoma. Persistent elevations of serum human chorionic gonadotropin (hCG) or alpha fetoprotein (AFP) are only an indication for salvage therapy if there is a definitive rise in the tumor marker. Elevated and rising serum hCG as the only evidence of recurrence can be because of cross reactivity with luteinizing hormone or usage of marijuana rather than progressive cancer. Elevated liver function tests can cause rising serum AFP. PMID:25993183

  17. Abnormal hCG levels in a patient with treated stage I seminoma: a diagnostic dilemma.

    LENUS (Irish Health Repository)

    Aherne, Noel J

    2008-01-01

    BACKGROUND: We report the case of a patient with treated Stage Ia seminoma who was found to have an elevated beta human chorionic gonadotrophin (hCG) on routine follow - up. This instigated restaging and could have lead to commencement of chemotherapy. CASE PRESENTATION: The patient was a bodybuilder, and following a negative metastatic work - up, admitted to injecting exogenous beta hCG. This was done to reduce withdrawal symptoms from androgen abuse. The patient remains well eight years post diagnosis. CONCLUSION: This case highlights the need for surgical oncologists to conduct vigilant screening of young male patients with a history of testicular germ cell tumours and who may indulge in steroid abuse.

  18. Triploid pregnancies, genetic and clinical features of 158 cases

    DEFF Research Database (Denmark)

    Joergensen, Mette W; Niemann, Isa; Rasmussen, Anders A;

    2014-01-01

    OBJECTIVE: The purpose of this study was to analyze the correlation between the genetic constitution and the phenotype in triploid pregnancies. STUDY DESIGN: One hundred fifty-eight triploid pregnancies were identified in hospitals in Western Denmark from April 1986 to April 2010. Clinical data...... than MMP cases (P cases, the possible karyotypes had similar frequencies, whereas, in PPM cases, 43% had the karyotype 69, XXX, 51% had the karyotype 69, XXY, and 6% had the karyotype 69, XYY. Molar phenotype was seen only in PPM cases. However, PPM cases with a nonmolar phenotype were...... also seen. For both parental genotypes, various fetal phenotypes were seen at autopsy. Levels of human chorionic gonadotropin in maternal serum were low in MMP cases and varying in PPM cases, some being as low as in the MMP cases. CONCLUSION: In a triploid pregnancy, suspicion of hydatidiform mole...

  19. Maternal use of fertility drugs and risk of cancer in children--a nationwide population-based cohort study in Denmark

    DEFF Research Database (Denmark)

    Hargreave, Marie; Jensen, Allan; Nielsen, Thor Schütt Svane;

    2015-01-01

    and follicle-stimulating hormone], gonadotropin-releasing hormone analogs, human chorionic gonadotropins, progesterone and other fertility drugs). We found no statistically significant association between maternal use of fertility drugs and risk for overall cancer in childhood or young adulthood. However......Large population-based studies are needed to examine the effect of maternal use of fertility drugs on the risk of cancer in children, while taking into account the effect of the underlying infertility. A cohort of 123,322 children born in Denmark between 1964 and 2006 to 68,255 women who had been...... evaluated for infertility was established. We used a case-cohort design and calculated hazard ratios (HRs) for cancer in childhood (0-19 years) and in young adulthood (20-29 years) associated with maternal use of six groups of fertility drugs (clomiphene, gonadotropins [i.e., human menopausal gonadotropins...

  20. The Luteal Phase after GnRHa Trigger-Understanding An Enigma

    DEFF Research Database (Denmark)

    Leth-Moller, Kathrine; Hammer Jagd, Sandra; Humaidan, Peter

    2014-01-01

    The luteal phase of all stimulated in vitro fertilization/intra-cytoplasmic sperm injection (IVF/ICSI) cycles is disrupted, which makes luteal phase support (LPS) mandatory. The cause of the disruption is thought to be the multifollicular development achieved during ovarian stimulation which...... phase defect. With the intro- duction of the gonadotropin-releasing hormone (GnRH) antagonist protocol, it became feasible to trigger final oocyte maturation and ovulation with a single bolus of GnRH agonist (GnRHa) as an alternative to human chorionic gonadotropin (hCG). GnRHa trig- gering presents...... several advantages, including the reduction in or even elimination of ovarian hyperstimulation syndrome. Despite the potential advantages of GnRHa trig- gering, previous randomized controlled trials reported a poor clinical outcome with high rates of early pregnancy losses, despite supplementation...