Sample records for chlorouracils

  1. Anaerobic biodegradation of halogenated and nonhalogenated N-, s-, and o-heterocyclic compounds in aquifer slurries (United States)

    Adrian, Neal R.; Suflita, Joseph M.


    The fate of several halogenated and nonhalogenated heterocyclic compounds in anoxic aquifer slurries was investigated Substrate depletion and methane formation were monitored in serum bottle incubations by HPLC and GC, respectively Pyridine, pyrimidine, thiophene, and furan were not mineralized following an 11-month incubation, but the corresponding carboxylated or oxygenated compounds were That is, >74% of the theoretically expected amount of methane was recovered from nicotinic acid, uracil, or 2-furoic acid Chlorinated derivatives, like 2 chloro- or 6-chloronicotinic acid, as well as 4 chloro- and 5-chlorouracil resisted mineralization However, 5-bromouracil was reductively dehalogenated to stoichiometric amounts of uracil, whereas 2-chloropyrimidine was metabolized to a more polar unidentified compound that resisted further anaerobic biodegradation Microorganisms acclimated to 5-bromouracil were unable to transform 4 chloro or 5 chlorouracil These findings illustrate how the structure of heterocyclic contaminants influences their susceptibility to anaerobic decay

  2. Experimental thermochemical study of fluoro-, chloro-, and bromo-derivatives of uracil

    International Nuclear Information System (INIS)

    Ribeiro da Silva, Manuel A.V.; Amaral, Luísa M.P.F.; Szterner, Piotr


    Highlights: ► Combustion calorimetry was used to determine Δ f H m ∘ (cr) of halo derivatives of uracil. ► Gas-phase Δ f H m ∘ of the studied compounds have been derived. ► The influence of the halogen atoms on the enthalpic stability of the halo-uracils is discussed. - Abstract: The values of the standard molar enthalpies of formation, of 5-fluorouracil, 5-chlorouracil, 5-bromouracil, and 6-chlorouracil, in the crystalline phase, at T = 298.15 K, were derived from the standard massic energies of combustion, in oxygen, at T = 298.15 K, measured by rotating bomb combustion calorimetry. The combination of the derived values of the standard molar enthalpies of formation, in the crystalline phase, and the literature values of the standard molar enthalpies of sublimation, allowed the calculation of the standard molar enthalpies of formation, in the gaseous phase, at T = 298.15 K, which are interpreted in terms of the influence of the halogen atoms upon the enthalpic stability of the halogen derivatives of uracil.

  3. Investigation of tritium tracer in nucleic acid components by hydrogenolysis of corresponding precursors. 7. Preparation of tritiated uracil, uridine-5'-mono-, di- and triphosphates of high molar activity

    Energy Technology Data Exchange (ETDEWEB)

    Myasoedov, N F; Sidorov, G V; Kuznetsova, O B; Frank-Kamenetskaya, M D; Lazurkina, T Yu; Orlova, V A


    Preparation of (5,6-/sup 3/H) uracil by hydrogenolysis of 5-bromo-6-chlorouracil with molar activity (1.59-1.63)x10/sup 15/ Bg/mol has been described. Hydrogenolysis reaction kinetics is studied, effect of the reaction conditions on molar activity of dehalogenation product is considered. Using enzyme fraction, separated from the E.coli cells tritium-labelled preparations of UMP, UTP and UTP from (5.6-/sup 3/H) uracil are made. Kinetics of enzymatic reactions is studied and conditions, permitting to synthesize both UMP and UTP and a mixture of nucleotides of different degree of phosphorylation in approximately equal amounts are selected. Separation of nucleotides labelled with tritium is conducted using ion exchange chromatography on DEAE cellulose. Molar activity of nucleotides labelled with tritium equals molar activity of initial (5.6-/sup 3/H) uracil, and radiochemical purity constitutes more than 95%.

  4. 5-Chlorouracil and 5-bromouracil acid-base equilibrium study in water and DMSO by NMR spectroscopy (United States)

    Abdrakhimova, G. S.; Ovchinnikov, M. Yu; Lobov, A. N.; Spirikhin, L. V.; Khursan, S. L.; Ivanov, S. P.


    Mechanism of 5-chloro- and 5-bromouracil deprotonation in water and dimethyl sulfoxide (DMSO) has been studied by the 13C and 1H NMR spectroscopy. NMR spectra were interpreted using DFT quantum chemical calculations at the CSGT-PCM-TPSSTPSS/6-311+G(d, p) level of theory. It was found that 5-chloro- (5ClU) and 5-bromouracil (5BrU) are present as a mixture of two anionic forms where the deprotonation is realized at the first (N1) and the third (N3) positions of the pyrimidine ring. N1 form is major for water-alkaline [xAN1/xAN3 (5ClU) = 0.65/0.35 and xAN1/xAN3 (5BrU) = 0.72/0.28, x - molar fraction] and the only one for DMSO solution.

  5. Interaction of electrons with biomolecules and development of a helium cluster source

    International Nuclear Information System (INIS)

    Denifl, S.


    In the main part of the present work electron interaction (attachment/ionization) with molecules of biological relevance has been studied in the electron energy range from about 0 to 70 eV. For these measurements a crossed neutral / high resolution electron beams apparatus in conjunction with a quadrupole mass spectrometer has been used. The present work should evaluate the description of the damage induced by high energy radiation since low secondary electrons with energies below 20 eV are created in a large amount in the interaction of the primary radiation with cell compounds. Thus dissociative electron attachment (DEA) and electron impact ionization near the threshold of biomulecules has been studied below 20 eV under isolated conditions. DEA to the DNA/RNA molecules thymine, cytosine and uracil has been carried out. As for most of the recently studied simple biomolecules (like isolated DNA bases, amino acids and sugars) no parent ion has been observed. It turned out that the most abundant fragment ions for DNA/RNA bases are the dehydrogenated bases. In addition to DNA/RNA bases also electron interaction with 6-Chlorouracil has been studied. Another part of this thesis is the construction of a He cluster source. Helium clusters are most difficult to produce as temperatures of about 10 K have to be reached in the stagnation chamber at the stagnation pressure of about 20 bar. The newly developed source allows achieving stagnation conditions for a helium cluster production. (author)