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Sample records for chirurgie cardiovasculaire est

  1. Activité physique, indice de masse corporelle, masse graisseuse par impédance: lequel de ces facteurs est le plus associé au risque cardiovasculaire pour nos patients?

    OpenAIRE

    SPRENG, L.

    2015-01-01

    Contexte: Les maladies cardiovasculaires représentent la première cause de mortalité en Suisse et dans le reste du monde depuis des années. Pourtant, elles sont le plus souvent causées par des facteurs de risque évitables et modifiables comme le tabac, l'inactivité physique, le surpoids, le stress, etc. Un dépistage est recommandé dès l'âge de 40 ans et chaque 5 ans chez toutes personnes asymptomatiques afin de pouvoir traiter au plus vite l'apparition d'un nouveau facteur de risqu...

  2. L’effet de l’extubation precoce apres chirurgie cardiaque pour la rehabilitation post opératoire

    Science.gov (United States)

    Cheikhrouhou, Hichem; Kharrat, Amine; Derbel, Rahma; Ellouze, Yesmine; Jmal, Karim; Ben Jmaa, Hela; Elkamel, Mohamed Ali; Frikha, Imed; Karoui, Abdelhamid

    2017-01-01

    Résumé Introduction La réhabilitation post opératoire en chirurgie cardiaque est basée sur une prise en charge médico-chirurgicale visant à diminuer la durée de séjour hospitalier et les coûts de soins de cette chirurgie à haut risque. L’extubation trachéale précoce, définie par une extubation avant la sixième heure, constitue la pierre angulaire du Fast-tracking. L’objectif de notre étude est d’évaluer dans notre institution la pratique du fast-track et notamment de l’extubation trachéale précoce en chirurgie cardiaque adulte programmée. Méthodes Nous avons conduit une étude descriptive incluant tous les patients âgés de plus 18 ans opérés consécutivement d’une chirurgie cardiaque programmée et qui ont été pris en charge en post-opératoire dans l’unité de réanimation post-opératoire du Service de chirurgie thoracique et cardiovasculaire du CHU HABIB BOURGUIBA de SFAX. Les critères d’inclusion des malades: âgés de 18 ans et plus, opérés d’une chirurgie cardiaque programmée, dont la prise en charge post-opératoire s’est faite dans l’unité de réanimation post-opératoire du service de chirurgie cardio-vasculaire et thoracique. Notre protocole d’anesthésie a été standardisé pour tous les patients: Propofol, Rémifentanil, Cisatracrium. Nous avons noté le délai d’extubation en post opératoire et les facteurs qui allongent ce délai d’extubation. Résultats Nous avons colligé 200 observations de malades consécutifs qui ont bénéficié d’une chirurgie cardiaque programmée. Parmi ces malades 115 étaient opérés pour pontage aorto-coronaire, 79 pour chirurgie valvulaire et 6 pour chirurgie combinée ou autre. Les caractéristiques démographiques sont comparables. En post-opératoire, nous avons pu extuber 152 soit 76% de nos malades avant la sixième heure. Quarante-huit patients n’ont pas pu être extubés avant la sixième heure. Les principales causes de l’échec de l

  3. Les manifestations cardiovasculaires chez les hemodialyses ...

    African Journals Online (AJOL)

    Le but de l'étude est d'analyser sur une période de 12 mois chez 75 patients en hémodialyse chronique, âgés de 38ans en moyenne, le les aspects cliniques, thérapeutiques et évolutifs des manifestations cardiovasculaires. La prévalence est de 87,20% chez les patients. Les signes fonctionnels les plus fréquents sont la ...

  4. L'effet de l'extubation precoce apres chirurgie cardiaque pour la ...

    African Journals Online (AJOL)

    ... une étude descriptive incluant tous les patients âgés de plus 18 ans opérés consécutivement d'une chirurgie cardiaque programmée et qui ont été pris en charge en post-opératoire dans l'unité de réanimation post-opératoire du Service de chirurgie thoracique et cardiovasculaire du CHU HABIB BOURGUIBA de SFAX.

  5. Est-il encore justifié et éthique de réaliser un bilan urodynamique avant la chirurgie de l’incontinence d’effort de la femme ?

    OpenAIRE

    Fritel, Xavier

    2015-01-01

    International audience; De nombreux chirurgiens ont l’habitude de demander un bilan urodynamique avant de réaliser une chirurgie de l’incontinence urinaire de la femme en particulier en cas d’incontinence mixte. Cet usage est conforme aux recommandations des sociétés savantes françaises (Collège National des Gynécologues et Obstétriciens Français et Association Française d’Urologie) publiées en 2010...

  6. Less is More? : Dépistage des facteurs de risque cardiovasculaire chez les enfants

    OpenAIRE

    Leyvraz, Magali; Bloetzer, Clemens; Bovet, Pascal; Suris, Joan-Carles; Simeoni, Umberto

    2016-01-01

    Les facteurs de risque cardiovasculaire se développent dès l’enfance et augmentent le risque de maladies cardiovasculaires à l’âge adulte. Sur la base de ces observations, le dépistage universel de facteurs de risque comme l’hypertension ou la dyslipidémie est parfois proposé chez les enfants. Faut-il recommander un dépistage universel des facteurs de risque des maladies cardiovasculaires dès l’enfance?

  7. Cholestérol alimentaire et morbi/mortalité cardiovasculaire

    Directory of Open Access Journals (Sweden)

    Hansel Boris

    2015-03-01

    Full Text Available Si l’hypercholestérolémie est un facteur de risque avéré des maladies cardiovasculaires, le rôle du cholestérol alimentaire dans la survenue de ces maladies reste discuté. À l’origine, les travaux d’Anitshkow chez le lapin ont montré qu’une alimentation riche en cholestérol se traduit par le développement de plaques d’athérome. Les études d’intervention chez l’homme ont examiné l’effet du cholestérol alimentaire et/ou des œufs sur les lipides plasmatiques, aboutissant à des résultats contradictoires. Elles ont également fait émerger la notion complexe d’hypo- et d’hyper-répondeur au cholestérol. L’épidémiologie observationnelle ne permet pas non plus de conclure à un effet délétère du cholestérol alimentaire chez l’homme. Néanmoins, chez les sujets diabétiques, une association positive entre la consommation et l’incidence des événements cardiovasculaires est retrouvée assez constamment, sans que l’on puisse l’expliquer de manière convaincante. Il n’existe pas d’essai clinique examinant l’impact des aliments riches en cholestérol sur la survenue des événements cardiovasculaires. Toutefois, les résultats des études MRFIT et PREDIMED ne vont pas dans le sens d’un effet délétère du cholestérol alimentaire. À l’inverse, les travaux réalisés sur le transporteur intestinal du cholestérol NPC1L1 sont en faveur de l’intérêt d’inhiber l’absorption du cholestérol pour réduire l’incidence des maladies cardiovasculaires. L’ensemble des données disponibles n’est pas en faveur d’un rôle clé du cholestérol alimentaire dans la survenue des maladies cardiovasculaires. Elles ne permettent donc pas d’en faire une cible prioritaire du régime à proposer pour prévenir les maladies cardiovasculaires.

  8. Dépistage des maladies cardiovasculaires chez des étudiants de l ...

    African Journals Online (AJOL)

    Introduction: Les maladies cardiovasculaires (MCV) constituent l'une des principales causes de mortalité dans les pays en développement. Le dépistage de ces dernières chez des jeunes est un défi dans la lutte contre leur expansion. Le but de cette étude était de dépister ces maladies au sein d'une population jeunes ...

  9. Etude clinique des facteurs de risque cardiovasculaire dans la ...

    African Journals Online (AJOL)

    l'attention des pouvoirs publics afin de définir une politique nationale de lutte contre l'HTA et les autres facteurs de risque cardiovasculaire. Mots clés : Hypertension artérielle, Epidémiologie, Facteurs de risque, Togo Clinical study of cardiovascular risk factors in adult population in Niamtougou (a secondary town of north ...

  10. Causes de report d'intervention chirurgicale en chirurgie pédiatrique ...

    African Journals Online (AJOL)

    Le but de se travail était de déterminer les causes de report du programme opératoire en chirurgie pédiatrique au CHU de Brazzaville. Il s'est agi d'une étude prospective descriptive réalisée dans le Service de chirurgie pédiatrique du CHU de Brazzaville entre juillet et décembre 2014, soit 6 mois. Elle a portée sur les ...

  11. Chirurgie in die Grieks-Romeinse era

    Directory of Open Access Journals (Sweden)

    François P. Retief

    2006-09-01

    Full Text Available In die Grieks-Romeinse era het mediese behandeling kenmerkend uit drie elemente bestaan, naamlik regimen (dieet en gesonde leefwyse, geneesmiddels en chirurgie – laasgenoemde alleen toegepas indien regimen en geneesmiddels onsuksesvol was. Bewyse van primitiewe chirurgie dateer terug na die Bronstydperk, en in Homerus se eposse is heelwat vermelding van die chirurgiese hantering van oorlogswonde, met tussenkoms van die gode. Met die koms van empiriese geneeskunde in die 5de eeu v.C. het chirurgie in die Hippokratiese Corpus prominent gefigureer met beduidende bydraes in veral die ortopediese veld en hoofbeserings. Uitbouing van anatomiese en fisiologiese kennis, gebaseer op disseksie van menslike kadawers in Alexandrië vanaf die laat 4de eeu v.C., het chirurgie ’n hupstoot gegee. Teen die Romeinse era vanaf die 2de eeu v.C. het snykundetegnieke (en -instrumente beduidend verbeter, maar is steeds oorwegend deur Griekse geneeshere beoefen. Van geneeshere is steeds verwag om al drie bovermelde terapeutiese modaliteite te bemeester, maar chirurgie het meer aansien verwerf en daar is al meer in onderafdelings van chirurgie soos oogheelkunde, vrouesiektes en verloskunde, blaaskwale en mond- en keelsnykunde gespesialiseer. Militêre geneeskunde was in die Romeinse Ryk ’n belangrike aktiwiteit, en het veral traumachirurgie uitgebou. Betreding van die buik- en toraksholtes was nie meer noodwendig fataal nie, en veeartsenykunde het tot stand gekom. Die eerste beduidende chirurgiehandboek ná die Hippokratiese Corpus is in die 1ste eeu n.C. deur Celsus opgestel. Vanaf die 3de eeu het die chirurgieberoep min vordering gemaak, die beroepstaal het mettertyd van Grieks na Latyn verander en kundigheid is later veral deur Islam-geneeshere na die Middeleeue en later oorgedra.

  12. Infections du site Operatoire en Chirurgie Orthopedique ...

    African Journals Online (AJOL)

    Mots clés: Infection site opératoire chirurgie orthopédique. English Title: Infections of the operative site in orthopedic surgery: clinical presentations, etiologies and management. English Abstract. Introduction: In orthopedic surgery, wound site infections constitute a difficult situation to the surgeon; and for the patient. The aim ...

  13. Acides gras oméga 3 et maladies cardiovasculaires : approches épidémiologiques

    Directory of Open Access Journals (Sweden)

    Ducimetiere Pierre

    2004-01-01

    Full Text Available La prise alimentaire d’acides gras oméga 3 a de nombreuses conséquences biologiques en particulier dans les domaines intéressant le risque cardiovasculaire (thrombose, métabolisme lipidique, inflammation. A partir de l’énorme littérature épidémiologique sur le sujet, on peut conclure que la consommation de poisson « gras » ou d’oméga 3 à longue chaîne a un effet protecteur sur la mortalité coronaire et plus généralement sur les maladies cardiovasculaires. Il existe des indications en faveur d’un effet similaire de l’apport en alphalinolénique (ALA. Les essais de prévention secondaire avec des oméga 3 à longue chaîne ont confirmé ces résultats mais les essais de conseils nutritionnels favorisant la consommation d’ALA ne sont qu’indicatifs. Il n’existe guère « d’evidence » en faveur du rôle des oméga sur le développement quantitatif de l’athérome alors que leur effet protecteur sur la vulnérabilité cardiaque aux arythmies est bien établie. Ces données expérimentales et cliniques fournissent une base biologique à la relation observée entre un apport augmenté de poisson ou d’oméga 3 et un risque abaissé de mort subite dans plusieurs études de cohortes.

  14. prise en charge des complications de la chirurgie partielle du larynx ...

    African Journals Online (AJOL)

    Introduction : Le cancer du larynx est un cancer fréquent occupant la première place des cancers des voies aéro-digestives chez l'homme en ... l'importance de la prise en charge des complications de la chirurgie partielle du cancer du larynx. ..... Mémoire pour l'obtention du certificat de capacité en orthophonie. Nantes.

  15. Exposition précoce au cholestérol et risque cardiovasculaire à l’âge adulte

    Directory of Open Access Journals (Sweden)

    Martin Jean-Charles

    2015-03-01

    Full Text Available Le lait maternel apporte au nourrisson l’équivalent de 5 à 10 fois la quantité de cholestérol de celle d’un adulte. En comparaison, les laits infantiles historiquement fabriqués à base de graisses laitières apportaient également du cholestérol au nourrisson mais en quantité moindre en raison d’une dilution avec les graisses végétales utilisées pour ajuster les apports. Ce contraste lait maternel/laits infantiles s’est encore creusé depuis une vingtaine d’années en raison d’un usage maintenant quasi-systématique des matières grasses végétales dans les formules commercialisées en Europe. Des études épidémiologiques suffisamment nombreuses comparant l’allaitement à l’alimentation aux formules soulignent un risque de maladie cardiovasculaire à l’âge adulte supérieur chez les enfants initialement alimentés aux formules. Des études animales, bien qu’incomplètes, suggèrent qu’il existe une programmation précoce du métabolisme du cholestérol liée à la nature des apports initiaux en ce stérol. Cette programmation est susceptible de retentir à long terme sur la cholestérolémie et le risque cardiovasculaire. Le point sur les données disponibles est livré dans cette revue, ainsi que les moyens possibles à mettre en œuvre pour répondre à la question de l’importance des apports en cholestérol en période néonatale.

  16. Qualite des soins du site operatoire en chirurgie orthopedique et ...

    African Journals Online (AJOL)

    Qualite des soins du site operatoire en chirurgie orthopedique et traumatologique au chu Sylvanus Olympio (so) ... été utilisée en prophylaxie ; le drainage et le lavage de la plaie opératoire en fin d'intervention n'ont pas été systématiques mais variaient selon les opérateurs et le type de chirurgie pratiqué ; les compresses ...

  17. Mécanismes des maladies cardiovasculaires chez le sujet noir africain :le vieillissement artériel précoce serait-il un déterminant ?

    OpenAIRE

    Lemogoum, Daniel

    2015-01-01

    RESUME DES TRAVAUXLa rigidification des gros troncs artériels (GTA) est physiologiquement liée à l’âge et s’accélère sous l’influence de certains facteurs de risque cardiovasculaire tels que l’hypertension artérielle (HTA), le diabète, le tabagisme, la dyslipidémie, l’obésité, la consommation excessive de sel, la sédentarité et l’hérédité. Le vieillissement artériel prématuré est un déterminant majeur du risque d’événements cardiovasculaires. Il se caractérise par une altération des propriété...

  18. anesthésie pour chirurgie de l'ulcère de Buruli : 8 mois d'expérience ...

    African Journals Online (AJOL)

    L'ulcère de Buruli (UB) est une affection invalidante due à Mycobacterium ulcerans qui atteint la peau et les tissus sous-jacents. Le traitement des lésions repose sur la chirurgie et l'antibiothérapie spécifique. Le but de cette étude rétrospective était d'évaluer les particularités de l'anesthésie réalisée pour cette chirurgie à ...

  19. Comportements à risque et maladies cardio-vasculaires. Comment infléchir le cours des choses?

    OpenAIRE

    Etienne, Anne-Marie; Krzesinski, Jean-Marie; André, Jean-François

    2014-01-01

    «Comportements à risques et maladies cardio-vasculaires, comment infléchir le cours des choses ?» Entre recommandations, croyances et attitudes des médecins, croyances, attitudes et vécu des patients, comment améliorer la prévention dans le domaine cardio-vasculaire ?

  20. Épidémiologie managériale des facteurs de risque cardiovasculaire ...

    African Journals Online (AJOL)

    Ces données appellent une enquête globale dans le district pour la planification et la mise en oeuvre urgente des interventions de santé publique pour réduire les complications. Mots clés: Prévalence, facteurs de risque cardiovasculaire et de cancers, Lot Quality Assurance Sampling, Zowla, Togo. English Title: Managerial ...

  1. Gradients socioéconomiques du risque cardiovasculaire chez les enfants et les adolescents canadiens

    Directory of Open Access Journals (Sweden)

    Y. Shi

    2016-01-01

    Full Text Available Introduction : Les maladies cardiovasculaires (MCV et leurs facteurs de risque présentent des gradients socioéconomiques clairs chez les adultes canadiens, mais présentent des ambiguïtés chez les enfants. L'objectif de cette étude est de vérifier l'existence ou non de gradients socioéconomiques dans les marqueurs physiologiques du risque de MCV chez les enfants et les adolescents canadiens. Méthodologie : À partir des données transversales combinées de l'Enquête canadienne sur les mesures de la santé 2007-2011, nous avons étudié, chez 2 149 enfants (6 à 11 ans et 2 073 adolescents (12 à 17 ans et selon le sexe, les marqueurs de risque cardiovasculaire suivants : excès de poids (y compris l'obésité, score de capacité aérobique (SCA, pression artérielle (PA, lipides sanguins (totaux, cholestérol LDL et HDL, triglycérides, métabolisme du glucose et protéine C réactive (CRP. Des analyses de régression logistique et de régression linéaire multidimensionnelles ont permis de dégager les tendances relatives au risque cardiovasculaire en fonction de la suffisance du revenu du ménage et du niveau de scolarité des parents, après ajustement en fonction de l'âge et de l'origine ethnique, et après stratification par groupe d'âge et par sexe. Résultats : La prévalence de l'obésité était sensiblement plus élevée chez les jeunes garçons que chez les jeunes filles (prévalence de 18,5 %, intervalle de confiance [IC] à 95 % : 15,6 à 21,5 contre 7,7 %, IC à 95 % : 5,2 à 10,3. Toutefois, des gradients socioéconomiques négatifs ont été observés en ce qui concerne le risque d'adiposité chez les jeunes filles et les adolescentes, et non chez les garçons. Parmi les enfants et les adolescents, les garçons étaient en meilleure condition physique que les filles (SCA moyen de 541, IC à 95 % : 534 à 546 contre 501, IC à 95 % : 498 à 505 chez les enfants; 522, IC à 95 % : 514 à 529 contre 460, IC à 95 % : 454

  2. Centre de chirurgie ambulatoire : implantation et développement au sein du Réseau Santé Valais en 2005

    OpenAIRE

    Buchard, Samuel

    2006-01-01

    Concept organisationel La chirurgie ambulatoire est avant tout un concept organisationnel centré sur le patient. "L'organisation est au centre du concept, le patient au centre de l'organisation" en rupture avec la pratique de l'hospitalisation traditionnelle où le patient est en attente des événements générés par les différents services (admission, radiologie, bloc opératoire, ...). Son développement suppose une évolution passant d'une simple distribution des ressources centrée sur l'acte chi...

  3. Place de la chirurgie dans la prise en charge des cancers du sein ...

    African Journals Online (AJOL)

    Etudier la place de la chirurgie dans la prise en charge des cancers du sein au centre hospitalier universitaire Yalgado Ouédraogo. Nous avons réalisé une étude prospective et descriptive sur dix (10) mois portant sur la place de la chirurgie dans le cancer du sein. Elle a eu pour cadre les services de ...

  4. La chirurgie réfractive de la cataracte: des technologies novatrices élargissent les choix des patients canadiens.

    Science.gov (United States)

    Potvin, Richard

    2016-11-01

    La chirurgie de la cataracte a toujours consisté à extraire le cristallin opacifié de l'œil pour le remplacer par une lentille artificielle. Cette intervention rend la vision du patient plus nette, sans pour autant corriger l'erreur de réfraction, c'est-à-dire la nécessité de porter des lunettes ou des lentilles cornéennes après l'opération. Les instruments diagnostiques et chirurgicaux modernes permettent désormais de corriger ces erreurs de réfraction au moment de l'opération, ce qui a fait émerger une nouvelle notion, celle de la chirurgie réfractive de la cataracte. Même si cette correction n'est pas nécessaire sur le plan médical, de nombreux patients se réjouissent de la possibilité de ne plus porter de lunettes ou d'en porter moins après l'opération, même s'ils doivent engager des frais. © 2016 The Canadian College of Health Leaders.

  5. Kystes hydatiques de la rate: chirurgie radicale ou conservatrice?

    Directory of Open Access Journals (Sweden)

    Abdelmalek Ousadden

    2010-06-01

    Full Text Available INTRODUCTION: Concernant l’hydatidose, la localisation splénique vient en 3ème position après le foie et les poumons. En l’absence de traitement médical réellement efficace, l’hydatidose splénique amène souvent à la chirurgie. L’apparition du traitement percutané et la tendance actuellement conservatrice de la chirurgie surtout pour une pathologie bénigne, remettent en cause la splénectomie radicale. METHODES: Notre travail rétrospectif, a porté sur 23 cas d’hydatidose splénique isolés ou multi-viscérale. Le diagnostic reposait principalement sur le couple échographie abdominale et sérologie hydatique. Ces patients ont bénéficié dans leur majorité d’une splénectomie ou d’une résection du dôme saillant du kyste. RESULTATS: La morbidité postopératoire a été plus importante en cas de résection du dôme saillant (une hémorragie d’origine splénique, 3 abcès sur cavité résiduelle et 2 récidives. La mortalité a été nulle. Nous proposons une classification qui permet de stratifier les indications opératoires en fonction du type de kyste hydatique de la rate. CONCLUSION: Le choix entre chirurgie radicale ou conservatrice reste difficile vu les contraintes de chacune, les complications postopératoires respectives et la diversité des situations cliniques. Notre classification facilite ce choix.

  6. Choc anaphylactique au cours de la chirurgie de kyste hydatique du foie : à propos d’un cas

    Directory of Open Access Journals (Sweden)

    Mohame Boughalem

    2010-08-01

    Full Text Available Nous rapportons l’observation d’un choc anaphylactique survenue au cours d’une chirurgie de kystes hydatiques hépatiques multiples. Il s’agit d’un enfant de 13 ans, de sexe féminin, sans antécédents pathologiques notables, en particulier allergique, programmé pour cure chirurgicale de kystes hydatiques hépatiques multiples. L’examen préopératoire est strictement normal. La patiente est classée ASA (American Society of Anaesthesiologists I. L’intervention est réalisée sous anesthésie générale. Au moment de la manipulation du deuxième kyste, une augmentation des pressions d’insufflation apparaît, avec des râles sibilants au niveau des deux champs pulmonaires et une désaturation, suivi d’un collapsus vasculaire avec hypotension artérielle 50/30. La prise en charge est débutée aussitôt par un remplissage vasculaire par du sérum salé 9%, suivi par des bolus d’adrénaline de 0,1mg à trois reprises, puis d’une perfusion continue à raison. L’adrénaline est arrêtée au bout de 48 heures et la patiente est adressée au service de chirurgie à j+4 de l’intervention. Le choc anaphylactique est une complication connue de la rupture intra péritonéale spontanée ou post-traumatique du kyste hydatique du foie. Sa survenue en peropératoire est devenue exceptionnelle, grâce aux mesures de prévention chirurgicale entretenues en peropératoire par l’éviction de manipulation du kyste, de le vider avant d’injecter le scolicide, et de ne pas l’administrer sous forte pression.

  7. L'obésité en consultation cardiologique à Lomé: prévalence et facteurs de risque cardio-vasculaire associés - étude chez 1200 patients

    Science.gov (United States)

    Pessinaba, Soulemane; Yayehd, Komlavi; Pio, Machiude; Baragou, Réné; Afassinou, Yaovi; Tchérou, Tchaa; Damorou, Findibé

    2012-01-01

    Introduction Introduction: Les objectifs de ce travail étaient de déterminer la fréquence de l'obésité et celle des autres facteurs de risque cardio-vasculaire chez ces patients obèses à Lomé (Togo). Méthodes Il s'est agi d'une étude multicentrique transversale de prévalence. Elle s'est déroulée du 05 septembre 2005 au 04 mars 2006 et a porté sur les malades vus en consultation externe dans 3 services de cardiologie de la commune de Lomé. Ont été inclus dans cette étude les malades ayant un surpoids selon les normes de l'OMS. Résultats Parmi 1200 patients vus en consultations, 779 (64,92%) avaient une surcharge pondérale. L’âge moyen était de 49,53 ± 17,24 ans. L'obésité était plus fréquente chez les femmes (79,49%) que chez les hommes (20,51%). Un antécédent d'obésité familiale (61,8%) était le principal facteur favorisant. Les autres facteurs de risque cardio-vasculaire retrouvés étaient: sédentarité (82% vs50% chez les non obèses), hypertension artérielle (54,8% vs 39,2%), alcool (50,9% vs 43,9%), dyslipidémie (34,5% vs 20%), diabète (30,9% vs 10,7%) et tabac (14,1% vs 20,3%). La différence était statistiquement significative entre les deux groupes. Les principales complications cardiovasculaires observées chez les obèses étaient: l'ischémie myocardique (26,7%), l'hypertrophie ventriculaire gauche (46,4%), la dilatation cavitaire cardiaque (30,1%) et les accidents vasculaires cérébraux (7,1%). Conclusion L'obésité est un problème de santé publique au Togo. Sa prévalence est très élevée et elle est le plus souvent associée aux autres facteurs de risque cardio-vasculaire. Des mesures préventives doivent être mises en jeu pour lutter contre ce facteur de risque. Mots clés: Obésité, prévalence, facteurs de risque. PMID:23133699

  8. Surgery of malignant pancreatic tumors; Chirurgie maligner Pankreastumoren

    Energy Technology Data Exchange (ETDEWEB)

    Loos, M.; Friess, H.; Kleeff, J. [Klinikum rechts der Isar, Technische Universitaet Muenchen (Germany). Chirurgische Klinik und Poliklinik

    2009-02-15

    Ductal adenocarcinoma is the most common malignant tumor of the pancreas. Despite great efforts in basic and clinical pancreatic cancer research, the prognosis remains poor with an overall 5-year survival rate of less than 5%. Complete surgical resection represents the only curative treatment option and 5-year survival rates of 20-25% can be achieved following curative resection and adjuvant chemotherapy. Although pancreatic surgery is considered one of the most technically demanding and challenging procedures, there has been constant progress in surgical techniques and advances in perioperative care with a modern interdisciplinary approach including anesthesiology, oncology, radiology and nursing. This has reduced morbidity and especially mortality rates in high-volume centers. Among extended resection procedures multivisceral and venous resections are technically feasible and should be considered if a complete tumor resection can be achieved. Multimodal regimens have shown promising results, however, only adjuvant chemotherapy is supported by solid evidence from randomized controlled trials. (orig.) [German] Das duktale Adenokarzinom ist der haeufigste maligne Pankreastumor. Trotz intensiver Anstrengungen auf dem Gebiet der Pankreasforschung konnte die Gesamtprognose in den vergangenen Jahren nicht entscheidend verbessert werden. Die einzige potenziell kurative Therapie ist die chirurgische Resektion. In Kombination mit einer adjuvanten Chemotherapie liegen die 5-Jahres-Ueberlebensraten aktuell bei 20-25%. Dank kontinuierlicher Weiterentwicklung der chirurgischen Operationstechnik und Verbesserungen der perioperativen Versorgung der Patienten mit einer engen interdisziplinaeren Zusammenarbeit (Chirurgie, Anaesthesie, Onkologie, Radiologie und Pflege) konnten die perioperative Morbiditaets- und Mortalitaetsraten in den vergangenen Jahren deutlich gesenkt werden. Unter den erweiterten Resektionsverfahren sind Venen- oder multiviszerale Resektionen technisch sicher

  9. Les avancees en chirurgie du cancer colo-rectal | Mouafo Tambo ...

    African Journals Online (AJOL)

    Revue Africaine de Chirurgie et Spécialités. Journal Home · ABOUT THIS JOURNAL · Advanced Search · Current Issue · Archives · Journal Home > Vol 10, No 3 (2016) >. Log in or Register to get access to full text downloads. Username, Password, Remember me, or Register. DOWNLOAD FULL TEXT Open Access ...

  10. Anesthésie d'un greffé cardiaque en chirurgie non cardiaque: à ...

    African Journals Online (AJOL)

    Le nombre et la durée de survie des patients transplantés cardiaque sont en augmentation. Une partie de ces patients se présentent régulièrement pour une chirurgie générale en dehors de la transplantation cardiaque. L'anesthésie chez ces patients peut être difficile en raison des particularités physiologiques du cœur ...

  11. Revue Africaine de Chirurgie et Spécialités - Vol 9, No 3 (2015)

    African Journals Online (AJOL)

    Les séquelles de brûlures en chirurgie pédiatrique au CHU Gabriel Touré de Bamako · EMAIL FULL TEXT EMAIL FULL TEXT DOWNLOAD FULL TEXT DOWNLOAD FULL TEXT. O Coulibaly, Y Coulibaly, I Toure, M Keita, Y Coulibaly, A Togo, I Diakite, L Kante, A Traore, BT Dembele, M Konate, G Diallo, 29-34 ...

  12. Dérivations bilio-digestives dans la chirurgie palliative du cancer de ...

    African Journals Online (AJOL)

    Le but de ce travail était d'évaluer l'importance de la dérivation bilio-digestive dans le traitement palliatif des cancers de la tête du pancréas. Il s'agissait d'une étude rétrospective sur une période de 11 ans et 6 mois (janvier 1999 à juin 2010) dans le service de chirurgie A du CHU du Point G. Les dossiers cliniques de 45 ...

  13. Krása v očích plastické chirurgie

    OpenAIRE

    Nováková, Hana

    2014-01-01

    Univerzita Karlova v Praze Institut sociologických studií Katedra sociologie Nováková Hana Bakalářská práce: Krása v očích plastické chirurgie Abstract The objective of this bachelor thesis is to examine the cosmetic surgery phenomenon from the sociological point of view. Social conditions under which cosmetic surgery are carried out in such quantity and popularity are defined and explored. Paper also contains a summary of patients' opinions about cosmetic surgery and explains the way they th...

  14. Rapport tour de taille/taille: Marqueur du risque cardio-vasculaire

    OpenAIRE

    Khélifi, N.; Falfoul, A.; Bhouri, S.; Boumefteh, S.; Jammoussi, H.; Berriche, O.; Amrouche, C.; Blouza, S.

    2012-01-01

    Objectif Déterminer la relation entre le rapport tour de taille/taille (RTT) et le profil métabolique, tensionnel et syndrome métabolique chez une population d’adultes obèses. Matériels et méthodes Étude transversale colligeant 134 adultes obèses ayant consulté à l’Unité de l’Obésité à l’Institut National de Nutrition de Tunis. Résultats L’âge moyen de nos patients est de 42 ± 12,5 ans. Le sexe Ratio (F/H) est de 11,18. 51,5 % présentent un syndrome métabolique(SM). Le RTT est...

  15. Analyse Pharmaceutique de la prescription des antibiotiques au service des brules et chirurgie plastique de l'Hopital Militaire d'Instruction Mohammed V, Maroc

    Science.gov (United States)

    Benziane, H.; Karfo, R.; Siah, S.; Taoufik, J.

    2011-01-01

    Summary L'infection est longtemps restée la principale cause de mortalité chez le brûlé grave. Ce travail a pour but d'évaluer la prescription des antibiotiques dans notre Service des Brûlés et Chirurgie Plastique, au regard du dossier d'autorisation de mise sur le marché (Résumé des caractéristiques du produit). Tous les patients sous antibiothérapie, au Service des Brûlés et Chirurgie Plastique de l'Hôpital Militaire d'Instruction Mohammed V de Rabat, Maroc, durant la période janvier 2008/mai 2009, ont été inclus. Il s'agit d'une étude rétrospective qui a analysé 41 dossiers de patients. Les antibiotiques utilisés par le service appartiennent à différentes familles. Les plus utilisés sont les bêta-lactamines (65%), glycopeptides (10,5%), aminosides (9%), quinolones (7%) et colistine (4,3%), avec 4,2% pour les autres classes d'antibiotiques (métronidazole 500 mg en perfusion, fluconazole injectable 100 mg/50 ml, rifampicine 600 mg en perfusion, sulfadiazine argentique crème, acide fusidique 2% crème, etc.); 70% des prescriptions sont non documentées. La voie injectable est prépondérante (89%). En tout, 227 ordonnances nominatives d'antibiothérapie ont été analysées: la posologie et les contre-indications ont été respectées par rapport à l'autorisation de mise sur le marché. Trois cas d'interactions médicamenteuses ont été relevés (fluconazole-rifampicine, fluconazole-Saccharomyces boulardii, amikacine-vancomycine). Ce constat montre l'importance de l'analyse pharmaceutique des prescriptions des antibiotiques dans un service utilisant des antibiotiques de la réserve hospitalière, donc actifs mais très toxiques. PMID:22396670

  16. Chirurgie de la lithiase biliaire par La célioscopie

    OpenAIRE

    BOUZIDI, M; CHEMLAL, L

    2015-01-01

    Durant la période 2009-2011; 59 patients ont été traités chirurgicalement par la coelioscopie et ils ont bénéficié d'une prise en charge au niveau du service de chirurgie « B » C.H.U Tlemcen. La lithiase biliaire reste une pathologie accès fréquent au sein de notre population qui atteint autant les sujets jeunes que les sujets âgée, autant de femme que d’homme. Le traitement chirurgical reste la seule solution pour cette maladie et malgré ses inconvénients la célioscopie ...

  17. Pronostic visuel et évolution réfractive après chirurgie de la cataracte congénitale avec implantation primaire: étude d'une série de 108 cas

    Science.gov (United States)

    Hafidi, Zouheir; Ibrahimy, Wafaa; Ahid, Samir; Handor, Hanan; Ouafae Cherkaoui, Lalla; Bencherif, Zahid; Laghmari, Mina; Ouazzanni, Btissam; Boutimzine, Noureddine; Daoudi, Rajae

    2013-01-01

    La cataracte congénitale constitue la cause la plus fréquente de cécité évitable chez les enfants. Le but de cette étude est d’évaluer le pronostic réfractif et fonctionnel, des enfants opérés de cataracte congénitale avec implantation. Il s'agit d'une étude rétrospective de 108 enfants, dont 85 cataractes bilatérales, 23 unilatérales opérés entre 2007 et 2011. La réfraction a été mesurée à 1 mois, 3 mois, 6 mois, 1 an, 2 ans, 3 ans et/ou 4 ans en post-opératoire. La meilleure acuité visuelle corrigée, ainsi que l'incidence des complications post-opératoires ont été analysé. L’âge moyen de la chirurgie était de 25 mois avec une durée moyenne de suivi de 3,17 ans. Les complications retrouvées étaient l'inflammation, la prolifération secondaire, et le glaucome. L'acuité visuelle (AV) moyenne corrigée finale était de 5,75/10e pour les formes bilatérales, et de 4,16/10e pour les unilatérales (p = 0,001). Les facteurs de mauvais pronostic retrouvés étaient l’âge tardif de la chirurgie, la densité de la cataracte et la survenue de complications (p = 0,001). L'incidence des complications post-opératoires était significativement plus élevé chez les enfants opérés à un jeune âge (p = 0,001). Les facteurs de mauvais pronostic visuel chez les enfants opérés pour cataracte congénitale avec implantation, sont représentés par le caractère unilatéral de la cataracte, l’âge tardif de la chirurgie, la densité de la cataracte et la survenue de complications post opératoires. PMID:24672622

  18. Procedure di controllo delle infezioni della ferita chirurgica nelle chirurgie generali della regione Piemonte

    Directory of Open Access Journals (Sweden)

    A. Castella

    2003-05-01

    Full Text Available

    Introduzione: le infezioni della ferita chirurgica (Surgical Site Infection=SSI presentano nelle realtà occidentali una frequenza relativa del 15 - 20% e tassi di incidenza dal 2 - 3% al 12 - 15%, in relazione alla classe di intervento. Concorrono all’insorgenza di SSI le condizioni cliniche del paziente, la durata della degenza pre operatoria, il tipo di intervento chirurgico, le pratiche assistenziali. Lo studio di queste ultime consente di individuare e analizzare quali procedure siano adottate fra quelle definite in letteratura di provata efficacia nella prevenzione delle SSI e quali siano di uso comune pur non esistendo per esse evidenza di efficacia. Obiettivo: descrivere e analizzare le procedure di controllo delle SSI messe in atto nelle chirurgie generali degli ospedali del Piemonte sotto il profilo assistenziale e ambientale.

    Materiale e metodi: lo studio prevede una settimana di osservazione nelle sale operatorie di chirurgia generale da parte di personale formato. Saranno indagati con schede appositamente strutturate: 1 tutti gli interventi, per rilevarne le caratteristiche e le misure di controllo pre e intraoperatorie rivolte al paziente; 2 i comportamenti degli operatori in sala; 3 i dati relativi al controllo ambientale. Al fine di poter confrontare oggettivamente le diverse situazioni sarà assegnato ad ogni valutazione un punteggio, sulla base delle evidenze di efficacia disponibili.

    Risultati: i dati raccolti consentiranno di evidenziare situazioni di inosservanza delle misure di controllo efficaci, disomogeneità di comportamenti fra presidi ospedalieri o intra “team” chirurgici, di avviare interventi di correzione attraverso produzione di documenti regionali e attività di formazione mirata.

  19. Traitement s?quentiel de la lithiase biliaire versus chirurgie seule: analyse par le score de propension

    OpenAIRE

    Toughrai, Imane; Ahid, Samir; Laalim, Said Ait; Majdoub, Karim Ibn; Mazaz, Khalid; Taleb, Khalid Ait

    2013-01-01

    Introduction L'objectif ?tant de comparer, r?trospectivement, les r?sultats du traitement combin? s?quentiel ? ceux de la chirurgie ouverte chez des patients pris en charge pour lithiase v?siculaire (LV) et lithiase de la voie biliaire principale (LVBP) durant la m?me hospitalisation M?thodes L??tude a concern? 132 patients pris en charge au CHU Hassan de F?s entre D?cembre 2003 et D?cembre 2011. Nos patients ont ?t? r?partis sur deux groupes th?rapeutiques. Le premier correspondant aux malad...

  20. Étude comparative de la mortalité cardiovasculaire dans la zone ICAPROS, une zone transfrontalière

    OpenAIRE

    Majérus, Paul

    2014-01-01

    L’objectif principal de cette étude est de comparer les niveaux de mortalité de la France, de la Belgique et d’une zone transfrontalière franco-belge, appelée zone ICAPROS, pour l’ensemble des maladies de l’appareil circulatoire (ICD-10 : I00-I99 ). Nous y étudions aussi la mortalité relative à deux causes secondaires incluses dans les maladies de l’appareil circulatoire, à savoir les infarctus aigus du myocarde (ICD-10 : I21-I22 ) et les maladies cérébrovasculaires (ICD-10 : I60-I69). La pri...

  1. EST-SSRs

    African Journals Online (AJOL)

    user1

    2012-09-04

    Sep 4, 2012 ... sequence repeats (EST-SSRs) for Musa and their applicability in authentication of a Musa ... understanding of Musa genetics, in marker-trait associations, thereby enhancing the effectiveness of breeding programs. Key words: EST ...... antioxidant, in cavendish banana. J. Agric. Food Chem. 48(3):844-. 848.

  2. Supplementation of fat grafts with adipose-derived regenerative cells in reconstructive surgery [Stammzellangereicherte Fetttransplantation in der rekonstruktiven Chirurgie

    Directory of Open Access Journals (Sweden)

    Herold, C.

    2012-09-01

    Full Text Available [english] Introduction: The fraction of regenerative cells in adipose tissue has been described to be even higher than in bone marrow. Adipose tissue itself is excessively available in most patients. Given that adipose tissue is abundant in majority of patients adipose derrived stem cells (ASCs have come under scrutiny for regenerative procedures in reconstructive surgery.Material and methods: ASCs were extracted by the Celution system for enrichment of fat grafts that were administered in patients with decreased wound healing, soft tissue or scar defects.Results: All patients were satisfied after reconstruction with ASCs augmented fat grafts and no side effects were observed. Discussion: The Celution system provides fast recovery of ASCs which can be immediately utilized for appropriate application. Since a high number of stem cells are harvested from fat tissue no expansion of cells is needed as described for bone marrow derived stem cells. Enrichment of fat graft with ASCs is of great interest due to their reported angiogenetic effect. The reported cases demonstrate the potential of ASCs in the field of regenerative medicine and encourage further application in reconstructive surgery.[german] Einleitung: Es konnte gezeigt werden, dass der Anteil regenerativer Zellen im Fettgewebe höher als im Knochenmark ist. Fettgewebe hingegen ist bei den meisten Patienten exzessiv vorhanden. Das legt den Einsatz von ASCs (adipose derived stem cells bei regenerativen Anwendungen in der rekonstruktiven Chirurgie nahe.Material und Methoden: Mit dem Celution System von Cytori Therapeutics Inc. prozessierte, ASC angereicherte Fetttransplantate werden an vier Patienten mit Weichteildefiziten und störenden Narben sowie Wundheilungsstörungen angewendet.Ergebnisse: Insbesondere bei Patienten mit Weichteildefiziten und Narben konnte eine suffiziente Volumenaugmentation und ansprechende Verbesserung der Narben erzielt werden. Es wurden keine Nebenwirkungen

  3. Le syndrome de sevrage nicotinique après chirurgie cardiaque: à ...

    African Journals Online (AJOL)

    L'agitation post-opératoire constitue une pathologie extrêmement fréquente. Les étiologies à évoquer en réanimation sont nombreuses. Le syndrome de sevrage à la nicotine est une cause possible mais rarement évoquée. Nous rapportons le cas d'un patient tabagique qui a été admis en unité de soins intensifs pour un ...

  4. EST Vocabulary Instruction

    Directory of Open Access Journals (Sweden)

    Célia D.S. Bell

    2012-05-01

    Full Text Available This study aims at contributing to the investigation on the instruction of EST (English for Science and Technology vocabulary, in terms of receptive use of the language. It evaluates the effectiveness of two teaching approaches to the acquisition of vocabulary. The first approach consisted of teaching vocabulary through the use of dictionaries, where the words were merely translated into the learners’ L1 or defined in the target language thus promoting superficial level of word processing. The second approach employed activities promoting deep level of word processing. Data were analysed quantitatively. Results indicated that the two approaches seem to have some equipotentiality, as far as EST vocabulary is concerned.

  5. Les maladies de coeur dans les recueils de recettes médicales et pharmaceutiques de la fin du Moyen Âge

    OpenAIRE

    Lorcin, Marie-Thérèse

    2014-01-01

    Les maladies cardio-vasculaires arrivent en tête des causes de décès dans l'Occident actuel, devançant le cancer qui inspire pourtant une terreur plus grande. C'est le privilège des populations bien nourries mais usées par le tabac, l'alcool, le stress, et surtout exposées, par l'allongement constant de la durée de la vie, à ces affections dont la fréquence croît avec l'âge. Les prouesses de la chirurgie cardiaque ne suffisent pas à renverser la tendance, puisque les maladies cardiovasculaire...

  6. Traitement séquentiel de la lithiase biliaire versus chirurgie seule: analyse par le score de propension

    Science.gov (United States)

    Toughrai, Imane; Ahid, Samir; Laalim, Said Ait; Majdoub, Karim Ibn; Mazaz, Khalid; Taleb, Khalid Ait

    2013-01-01

    Introduction L'objectif étant de comparer, rétrospectivement, les résultats du traitement combiné séquentiel à ceux de la chirurgie ouverte chez des patients pris en charge pour lithiase vésiculaire (LV) et lithiase de la voie biliaire principale (LVBP) durant la même hospitalisation Méthodes L’étude a concerné 132 patients pris en charge au CHU Hassan de Fès entre Décembre 2003 et Décembre 2011. Nos patients ont été répartis sur deux groupes thérapeutiques. Le premier correspondant aux malades ayant eu une sphincterotomie endoscopique première suivie d'une cholécystectomie par voie cœlioscopique. Le deuxième incluant les malades ayant eu un traitement exclusivement chirurgical. La comparaison des deux groupes a porté sur les résultats en termes de morbi-mortalité, de séjour post opératoire et d'efficacité du traitement. Résultats L'appariement des deux groupes thérapeutiques a permis de retenir 64 patients. L’âge moyen a été de 54 ans ± 14 avec un sexe ratio F/H de 2. Quatorze pourcents de nos malades avaient des tares associées. L'ictère ± angiocholite a été noté chez 76,6% de nos patients. Des anomalies du bilan hépatique ont été relevées chez plus de 70% des malades. L’échographie abdominale et le scanner ont été réalisés chez 97% et 30% des malades respectivement. La médiane du séjour post opératoire a été de 2,5 jours pour le premier groupe versus 5 jours pour le deuxième. Nous avons eu un cas de décès post opératoire dans le premier groupe. Les complications post opératoires ont été à type d'un cas de fistule biliaire et 2 cas de sepsis dans le premier groupe. Deux cas de lithiase résiduelle ont été observés dans le groupe de chirurgie seule. Conclusion Le choix d'une stratégie de prise en charge de la lithiase biliaire devra prendre en considération le plateau technique disponible et le degré d'expertise des opérateurs. Dans l'attente d'une maîtrise parfaite du traitement de la

  7. Le statut matrimonial et la qualité des liens sociaux des personnes souffrant de maladies cardiovasculaires influent-ils sur les inégalités liées aux changements des comportements à risque?

    OpenAIRE

    Tchicaya, Anastase; Baumann, Michèle

    2016-01-01

    Les maladies cardiovasculaires constituent l’une des principales causes de morbidité et de mortalité dans les pays développés. Liées aux modes de vie et aux choix alimentaires, elles représentent un problème sociétal et de santé publique majeur. Dans une étude de suivi des patients ayant passé un examen des coronaires 5 ans plus tôt au Luxembourg (n=1289), notre communication tentera de mettre en évidence l’impact du statut matrimonial et des liens sociaux sur la prise de conscience des fa...

  8. Troubles du sommeil, symptômes anxio-dépressifs et risque cardio-vasculaire chez les hypertendus noirs africains: étude transversale de 414 hypertendus suivis en ambulatoire au CHU de Ouagadougou (Burkina Faso)

    Science.gov (United States)

    Yaméogo, Nobila Valentin; Samadoulougou, André; Kagambèga, Larissa Justine; Yaméogo, Aimé Arsène; Ilboudo, Eric; Millogo, Georges; Kologo, Jonas; Toguyeni, Jean Yves; Simporé, Jacques; Zabsonré, Patrice

    2015-01-01

    Introduction Dans le but de déterminer la fréquence des troubles du sommeil, de l'anxiété et de la dépression, et de rechercher l'existence d'une relation entre ces troubles et le risque cardio-vasculaire global chez les hypertendus noirs africains, nous avons réalisé une étude transversale de mai 2010 à mars 2011 à l'unité de consultation externe du service de cardiologie du CHU-Yalgado Ouédraogo de Ouagadougou qui a inclut 414 hypertendus adultes suivis en ambulatoire. Après un examen clinique à la recherche des facteurs de risque cardio-vasculaire, deux auto-questionnaires ont été administrés. Méthodes Le questionnaire de l’«European Sleep Center » pour la recherche des troubles du sommeil et, l’échelle «Hospital Anxiety and Depression Scale » de Zigmond et Snaith pour la recherche de l'anxiété et la dépression. Le diagnostic du syndrome d'apnée du sommeil était clinique et basé sur la présence des 4 symptômes principaux: hypersomnolence diurne, éveils nocturnes fréquents avec nycturie, asthénie matinale avec ou sans céphalées et ronflements importants. Le risque cardio-vasculaire global était calculé grâce à l’équation d'Anderson tirée de l’étude de Framingham. L'analyse des données a été réalisée par le logiciel SPSS version 17. La comparaison des variables a été effectuée grâce au test de Khi 2 pour les variables qualitatives et au test «t» de Student pour les variables quantitatives. Le seuil de signification a été fixé à 5%. Résultats L’échantillon était composé de 414 patients dont 248 femmes (59,9%). L’âge moyen était de 54,6 ± 9,3 ans. Les troubles du sommeil étaient retrouvés dans 72,2% des cas. Ils étaient dominés par l'insomnie (49,2%), le syndrome d'apnée du sommeil (33,5%) et le syndrome des jambes sans repos (25,8%). L'anxiété était retrouvée dans 37,1% des cas et la dépression dans 16,6% des cas. Le risque cardio-vasculaire global était faible dans 21,0%, mod

  9. CitEST libraries

    Directory of Open Access Journals (Sweden)

    Maria Luísa P. Natividade Targon

    2007-01-01

    Full Text Available In order to obtain a better understanding of what is citrus, 33 cDNA libraries were constructed from different citrus species and genera. Total RNA was extracted from fruits, leaves, flowers, bark, seeds and roots, and subjected or not to different biotic and abiotic stresses (pathogens and drought and at several developmental stages. To identify putative promoter sequences, as well as molecular markers that could be useful for breeding programs, one shotgun library was prepared from sweet orange (Citrus sinensis var. Olimpia. In addition, EST libraries were also constructed for a citrus pathogen, the oomycete Phythophthora parasitica in either virulent or avirulent form. A total of 286,559 cDNA clones from citrus were sequenced from their 5’ end, generating 242,790 valid reads of citrus. A total of 9,504 sequences were produced in the shotgun library and the valid reads were assembled using CAP3. In this procedure, we obtained 1,131 contigs and 4,083 singletons. A total of 19,200 cDNA clones from P. parasitica were sequenced, resulting in 16,400 valid reads. The number of ESTs generated in this project is, to our knowledge, the largest citrus sequence database in the world.

  10. Chirurgie des grassmanniennes

    CERN Document Server

    Lafforgue, Laurent

    2003-01-01

    Les compactifications diverses de variétés de modules sont un thème important et récurrent des mathématiques modernes, et elles connaissent un grand nombre d'applications. Ce livre traite le cas de cellules de Schubert minces, qui sont de sous-variétés naturelles de grassmanniennes. L'auteur a été amené à traiter ces questions par un cas particulier lié à ses travaux sur le programme de Langlands. Dans cette monographie, il en développe une théorie plus systématique, présentant le fortes similarités avec celle des modules du courbes stables. The various compactifications of moduli spaces are an important recurrent theme of modern mathematics, and they have a large number of applications. This book treats the case of thin Schubert varieties, which are natural subvarieties of Grassmannians. The author was led to these questions by a particular case linked to his work on the Langlands program. In this monograph, he develops the theory in a more systematic way, which exhibits strong similarities...

  11. Chirurgie angeborener Herzfehler

    Science.gov (United States)

    Schreiber, Christian; Libera, Paul; Lange, Rüdiger

    Störungen der embryonalen Entwicklung in der frühen Phase der Schwangerschaft können zu Fehlbildungen am Herz- und Gefäßsystem führen. Die Häufigkeit liegt bei 0.8-1 % aller lebend geborenen Kinder. In Deutschland werden jedes Jahr etwa 6.000 Kinder mit einem Herzfehler geboren (Quelle: http://www.kompetenznetzahf.de). Das Spektrum reicht von einfachen Fehlern, die das Herz-Kreislauf-System wenig beeinträchtigen, bis zu sehr schweren Herzerkrankungen, die unbehandelt zum Tode führen. Fortschritte der Kinderkardiologie, Herzchirurgie und Anästhesie ermöglichen heute ein Überleben bei über 90 % der Patienten. Auch die spezialisierte Pränataldiagnostik (vorgeburtliche Diagnostik) ermöglicht schon die frühe Weichenstellung für mögliche Therapieoptionen. Bei der chirurgischen Therapie ist jedoch festzuhalten, dass ein Herzfehler entweder korrigierend behandelt wird oder nur "palliiert“ werden kann. Bei letzterer Therapie wird bei einem Patienten eine medizinische Maßnahme durchgeführt, die nicht die Herstellung normaler Körperfunktionen zum Ziel hat, sondern in Anpassung an die physiologischen Besonderheiten des Patienten dessen Zustand lediglich stabilisiert und optimiert. Dies kann beispielsweise bei einer nicht korrigierbaren angeborenen Fehlbildung notwendig sein, bei der lediglich eine funktionelle Herzkammer vorhanden ist (z. B. hypoplastisches Linksherz). Hierbei muss eine prothetische Verbindung zur Lungenstrombahn in der Folgezeit entfernt werden.

  12. Laser-chirurgie

    OpenAIRE

    КУБЛАНОВА А.А.; САПАРГАЛИЕВА С.А.; ДЕНИСОВА О.С.

    2015-01-01

    Das Wort „Laser“ steht für „Light Amplification by Stimulated Emission of Radiation“. Laserstrahlen sind starke Lichtstrahlen, die durch elektrische Stimulation eines bestimmtes Stoffs (fest, flüssig oder gasförmig) erzeugt werden.Laser werden nach dem Stoff benannt, mit dem sie den Strahl erzeugen. Weitverbreitete Laser sind u. a. Kohlendioxidlaser und Argonlaser.Die Kombination aus der Stärke eines Lichtstrahls und der Fähigkeit, diesen auf eine kleine Fläche zu konzentrieren, verhilft Lase...

  13. EST2Prot: Mapping EST sequences to proteins

    Directory of Open Access Journals (Sweden)

    Lin David M

    2006-03-01

    Full Text Available Abstract Background EST libraries are used in various biological studies, from microarray experiments to proteomic and genetic screens. These libraries usually contain many uncharacterized ESTs that are typically ignored since they cannot be mapped to known genes. Consequently, new discoveries are possibly overlooked. Results We describe a system (EST2Prot that uses multiple elements to map EST sequences to their corresponding protein products. EST2Prot uses UniGene clusters, substring analysis, information about protein coding regions in existing DNA sequences and protein database searches to detect protein products related to a query EST sequence. Gene Ontology terms, Swiss-Prot keywords, and protein similarity data are used to map the ESTs to functional descriptors. Conclusion EST2Prot extends and significantly enriches the popular UniGene mapping by utilizing multiple relations between known biological entities. It produces a mapping between ESTs and proteins in real-time through a simple web-interface. The system is part of the Biozon database and is accessible at http://biozon.org/tools/est/.

  14. galaxieEST: addressing EST identity through automated phylogenetic analysis.

    Science.gov (United States)

    Nilsson, R Henrik; Rajashekar, Balaji; Larsson, Karl-Henrik; Ursing, Björn M

    2004-07-05

    Research involving expressed sequence tags (ESTs) is intricately coupled to the existence of large, well-annotated sequence repositories. Comparatively complete and satisfactory annotated public sequence libraries are, however, available only for a limited range of organisms, rendering the absence of sequences and gene structure information a tangible problem for those working with taxa lacking an EST or genome sequencing project. Paralogous genes belonging to the same gene family but distinguished by derived characteristics are particularly prone to misidentification and erroneous annotation; high but incomplete levels of sequence similarity are typically difficult to interpret and have formed the basis of many unsubstantiated assumptions of orthology. In these cases, a phylogenetic study of the query sequence together with the most similar sequences in the database may be of great value to the identification process. In order to facilitate this laborious procedure, a project to employ automated phylogenetic analysis in the identification of ESTs was initiated. galaxieEST is an open source Perl-CGI script package designed to complement traditional similarity-based identification of EST sequences through employment of automated phylogenetic analysis. It uses a series of BLAST runs as a sieve to retrieve nucleotide and protein sequences for inclusion in neighbour joining and parsimony analyses; the output includes the BLAST output, the results of the phylogenetic analyses, and the corresponding multiple alignments. galaxieEST is available as an on-line web service for identification of fungal ESTs and for download / local installation for use with any organism group at http://galaxie.cgb.ki.se/galaxieEST.html. By addressing sequence relatedness in addition to similarity, galaxieEST provides an integrative view on EST origin and identity, which may prove particularly useful in cases where similarity searches return one or more pertinent, but not full, matches and

  15. La vitamine E : état actuel des connaissances, rôle dans la prévention cardio-vasculaire, biodisponibilité

    Directory of Open Access Journals (Sweden)

    Leger Claude-Louis

    2000-05-01

    Full Text Available Les propriétés anti-oxydantes de la vitamine E – qui font l’objet d’une large majorité des travaux effectués actuellement sur cette vitamine – n’ont été reconnues qu’au cours des années 50. La vitamine E avait cependant été découverte quelque trente ans plus tôt. Mais, paradoxe intéressant, son rôle comme micronutriment essentiel n’a été reconnu qu’à la fin des années 60. Cette vitamine jouit d’un certain nombre de particularités. C’est l’anti-oxydant majeur des milieux lipidiques (huiles, membranes biologiques, lipoprotéines. Elle présente différentes formes moléculaires : des vitamères a, b, g et d, chaque vitamère existant sous différentes formes stéréo-isomériques dont une seule, parmi les huit formes possibles, est naturelle : la forme RRR (figure 1. La molécule présente deux parties : un noyau 6-OH-chromane qui possède la fonction anti-oxydante, et une chaîne latérale à 16 atomes de carbone de structure isoprénique, celle-ci définissant deux grandes familles : les tocophérols à chaîne latérale saturée et les tocotriénols avec une chaîne latérale présentant trois doubles liaisons. Cette chaîne latérale détermine la lipophilicité et la stéréochimie de la molécule. C’est la stéréochimie de la molécule qui détermine à son tour la reconnaissance préférentielle de la forme RRR-a-tocophérol par un transporteur hépatique spécifique dont le rôle physiologique est aujourd’hui reconnu. Chacune de ces formes moléculaires (le nombre théorique est de 64 peut être caractérisée par une activité vitaminique E, définie par référence au pouvoir anti-abortif chez la rate gestante. Les tableaux 1 et 2 donnent cette activité en fonction de la molécule considérée. La forme naturelle de l’a-tocophérol, le RRR-a-tocophérol, possède l’activité la plus élevée, alors que la stéréo-isomérie de la molécule n’exerce aucun effet sur ses propriétés anti

  16. Evaluation de la formation des résidents en chirurgie générale et digestive en Tunisie

    Science.gov (United States)

    Mahmoudi, Ammar; Noomen, Faouzi; Nasr, Mohamed; Zouari, Khadija; Hamdi, Abdelaziz

    2015-01-01

    Introduction De nombreux moyens sont mis à disposition des résidents en chirurgie générale et digestive pour assurer leur formation théorique et pratique. Cependant, le niveau d'utilisation de ces différents outils et leur impact sur la formation des résidents n'ont jamais été évalués. L'objectif de notre étude était d’étudier l’état des lieux des moyens de formation utilisés par les résidents pour évaluer leurs degrés de satisfaction et leurs propositions en vue d'améliorer leur formation. Méthodes Un questionnaire anonyme a été distribué aux résidents de chirurgie générale et digestive de l'année 2012-2013. Ce questionnaire portait sur les caractéristiques démographiques, les ressources pédagogiques, ainsi que le cursus médical et universitaire. Une évaluation de la formation ainsi qu'un recueil des propositions faites en vue d'améliorer leurs formations étaient réalisées. Résultats Cinquante résidents sur 83 ont répondu au questionnaire. L'orientation de carrière la plus fréquente était l'hospitalo-universitaire dans 70% des cas. La pratique quotidienne et l'internet étaient les deux ressources pédagogiques les plus utilisées. La formation chirurgicale était jugée satisfaisante par seulement 10% des répondants. Parmi l'ensemble des propositions faites, l'apprentissage sur simulateur chirurgical, l'existence d'un ouvrage national de référence, et l'institution d'un tutorat par un chirurgien senior recueillaient plus de 80% d'avis favorable. Conclusion La majorité des résidents jugent leur formation non satisfaisante. Une meilleure information sur les ressources déjà existantes, un renforcement du compagnonnage et un accès plus large à un apprentissage sur simulateur chirurgical permettraient de diminuer ce sentiment d'insatisfaction. PMID:26587174

  17. EST databases and web tools for EST projects.

    Science.gov (United States)

    Shen, Yao-Qing; O'Brien, Emmet; Koski, Liisa; Lang, B Franz; Burger, Gertraud

    2009-01-01

    This chapter outlines key considerations for constructing and implementing an EST database. Instead of showing the technological details step by step, emphasis is put on the design of an EST database suited to the specific needs of EST projects and how to choose the most suitable tools. Using TBestDB as an example, we illustrate the essential factors to be considered for database construction and the steps for data population and annotation. This process employs technologies such as PostgreSQL, Perl, and PHP to build the database and interface, and tools such as AutoFACT for data processing and annotation. We discuss these in comparison to other available technologies and tools, and explain the reasons for our choices.

  18. School version of ESTE EU

    International Nuclear Information System (INIS)

    Carny, P.; Suchon, D.; Chyly, M.; Smejkalova, E.; Fabova, V.

    2008-01-01

    ESTE EU is information system and software for radiological impacts assessment to the territory of the country in case of radiation accident inside/outside of the country .The program enables to model dispersion of radioactive clouds in small-scale and meso-scale. The system enables the user to estimate prediction of the source term (release to the atmosphere ) for any point of radiation/nuclear accident in Europe (for any point of the release, but especially for the sites of European power reactors ). The system enables to utilize results of real radiological monitoring in the process of source term estimation. Radiological impacts of release to the atmosphere are modelled and calculated across the Europe and displayed in the geographical information system (GIS). The school version of ESTE EU is intended for students of the universities which are interested in or could work in the field of emergency response, radiological and nuclear accidents, dispersion modelling, radiological impacts calculation and urgent or preventive protective measures implementation. The school version of ESTE EU is planned to be donated to specialized departments of faculties in Slovakia, Czech Republic, etc. System can be fully operated in Slovak, Czech or English language. (authors)

  19. School version of ESTE EU

    International Nuclear Information System (INIS)

    Carny, P.; Suchon, D.; Chyly, M.; Smejkalova, E.; Fabova, V.

    2009-01-01

    ESTE EU is information system and software for radiological impacts assessment to the territory of the country in case of radiation accident inside/outside of the country .The program enables to model dispersion of radioactive clouds in small-scale and meso-scale. The system enables the user to estimate prediction of the source term (release to the atmosphere ) for any point of radiation/nuclear accident in Europe (for any point of the release, but especially for the sites of European power reactors ). The system enables to utilize results of real radiological monitoring in the process of source term estimation. Radiological impacts of release to the atmosphere are modelled and calculated across the Europe and displayed in the geographical information system (GIS). The school version of ESTE EU is intended for students of the universities which are interested in or could work in the field of emergency response, radiological and nuclear accidents, dispersion modelling, radiological impacts calculation and urgent or preventive protective measures implementation. The school version of ESTE EU is planned to be donated to specialized departments of faculties in Slovakia, Czech Republic, etc. System can be fully operated in Slovak, Czech or English language. (authors)

  20. Qui est Méduse?

    Directory of Open Access Journals (Sweden)

    Julia Kristeva

    2015-04-01

    Full Text Available Une belle histoire de têtes coupées traverse l'Antiquité grecque : celle des Gorgones, trois monstres ailés au corps de femme et à la chevelure de serpents, dont le regard changeait en pierre celui qui se risquait à les contempler – Méduse, Euryalé et Sthéno. Pour commencer, Méduse est une jeune fille qui se fait remarquer : séduite puis violée par Poséidon, elle se révèle fertile puisqu'elle accouche de faux jumeaux, le cheval Pégase et le géant Chrysaor. Une longue fable la transforme en terrible puissance. Pour simplifier, disons que le monstre est par deux fois tué : Persée assassine d'abord Méduse pour assurer la protection de sa mère, Danaé, importunée par le roi Polydectès. Ensuite, il délivre Andromède des liens méduséens qui la retiennent et, pour ce faire, tranche la tête de la Gorgone. La mer se teinte de sang, tandis que le vainqueur tient à l'écart l'épouvantail de Méduse, de peur que ne soient changés en statues de pierre ceux qui s'exposeraient à sa vue.

  1. La parole est à vous

    CERN Multimedia

    Staff Association

    2015-01-01

    Nous avons identifié, ci-dessous, 3 grands chapitres pour lesquelles bon nombre d’entre vous se posent des questions et ont fréquemment sollicités leurs délégués, qui n’ont pas toujours pu donner de réponses : La retraite et sa préparation. L’affiliation aux régimes sécurité sociale de nos états hôtes et ses conséquences au moment de la retraite. La fiscalité en général. Le service juridique, que nous avons interrogé récemment, n’est pas toujours informé de vos interrogations et de ce fait, il n’a pas encore pu investiguer sur ces questions auprès des services compétents. Il nous a suggéré de vous interroger afin de collecter vos questions, de les synthétiser, afin de les transmettre...

  2. SNP-finding in pig mitochondrial ESTs

    DEFF Research Database (Denmark)

    Scheibye-Alsing, Karsten; Cirera Salicio, Susanna; Gilchrist, M.J.

    2008-01-01

    The Sino-Danish pig genome project produced 685 851 ESTs (Gorodkin et al. 2007), of which 41 499 originated from the mitochondrial genome. In this study, the mitochondrial ESTs were assembled, and 374 putative SNPs were found. Chromatograms for the ESTs containing SNPs were manually inspected...

  3. Villa d'Este i Tivoli

    DEFF Research Database (Denmark)

    Bach-Nielsen, Carsten

    2012-01-01

    Om Villa d'Este, en renæssancehave ved Rom, der tematiserer Herkules' valg mellem dydens og lastens vej......Om Villa d'Este, en renæssancehave ved Rom, der tematiserer Herkules' valg mellem dydens og lastens vej...

  4. Ortodoncia estética invisible

    OpenAIRE

    Chávez Sevillano, Manuel Gustavo; Soldevilla Galarza, Luciano

    2014-01-01

    El adulto suele ofrecer resistencia a los convencionales tratamientos ortodóncicos, debido a la necesidad de llevar brackets visibles, tanto metálicos como estéticos de porcelana. El concepto de Ortodoncia Estética Invisible u Ortodoncia Lingual cubre las expectativas de este tipo de pacientes. La técnica multibrackets con aparatología lingual tiene aproximadamente 25 años de desarrollo y con la experiencia de los casos tratados, se ha llegado a la concepción de una técnica completamente prot...

  5. A caminho da estética

    Directory of Open Access Journals (Sweden)

    Amaral Filho, Fausto dos Santos

    2007-01-01

    Full Text Available O presente estudo tenta compreender, ainda que de maneira sumária, quais os caminhos que a Estética tomou; desde Hegel e a dedução filosófica dos conceitos, até a corrente, influenciada por Wittgenstein, da análise da liguagem comum, acabando por apontar, diante das dificuldades dos referidos caminhos, para a Estética da circularidade; tanto a de Heidegger quanto a de George Dickie

  6. EST Reading Curriculum & Instruction: An Alignment Analysis

    Directory of Open Access Journals (Sweden)

    Lubna Ali Mohammed

    2015-02-01

    Full Text Available In order for a curriculum to achieve its goals, it is imperative that the curriculum is coherent at all levels. In order to determine the coherency of a curriculum, the alignment of its theoretical underpinning and the label of a curriculum is one of the aspects that can be examined. The purpose of the current study was to examine if the theoretical grounding of the Yemeni EST senior secondary curriculum is in alignment with its communicative-based curriculum label. In line with its label, the reading curriculum embedded in the EST curriculum should be developed and designed based on the Communicative Language Teaching (CLT, which should be reflected in the EST reading instructional practice. In analysing the alignment of the communicative language teaching approach in the EST senior secondary school reading curriculum, the primary focus of the analysis was on the major SLA theories and L2 reading theories and the related instructional approaches. The data for this study were collected via an ESP textbook review as well as from the observation of the EST reading classroom instruction. The findings show that the EST senior secondary reading curriculum was not developed based on the socio-cognitive and socio-cultural theories which are the grounding principles of the communicative approach. Instead, the findings show that the EST senior secondary reading curriculum was primarily developed based on the cognitive information processing theory, in which teaching instruction reflects the student as an individual information processor and the focus on teaching vocabulary and grammatical rules using reading texts in non-communicative manner. Keywords: Communicative Approach, SLA Theories, L2 Reading Theories

  7. Turismo y estética formativa

    Directory of Open Access Journals (Sweden)

    Clara Inés Sánchez Arciniegas

    2002-11-01

    Full Text Available ¿Existe alguna armonía entre el conocimiento y la estética? Sin duda alguna el hombre con conocimiento y sin virtud de la estética es un autómata; este necesita de las diferentes manifestaciones en las que se encuentra plasmada y necesita recordarlas continuamente. La estética como doctrina de conocimiento no es reciente, en el siglo xviii cuando por primera vez se define teóricamente hasta nuestros días en la Universidad actual. Esta última aparentemente menos evidente debido a las nuevas formas de aprendizaje con herramientas tecnológicas muy avanzadas y contraria a la imagen del universitario que hace dos siglos como complemento a su educación se distraía a través del Gran Tour, partida que tiene el turismo para fomentar el goce estético. ¿Cuál es entonces la relación entre distracción y formación? Se debe entonces reflexionar y comprender que el conocimiento interdisciplinario y la metodología debe procurar en los hombres actitudes adecuadas frente a la cultura y el arte a través del conocimiento.

  8. EST-PAC a web package for EST annotation and protein sequence prediction

    Directory of Open Access Journals (Sweden)

    Strahm Yvan

    2006-10-01

    Full Text Available Abstract With the decreasing cost of DNA sequencing technology and the vast diversity of biological resources, researchers increasingly face the basic challenge of annotating a larger number of expressed sequences tags (EST from a variety of species. This typically consists of a series of repetitive tasks, which should be automated and easy to use. The results of these annotation tasks need to be stored and organized in a consistent way. All these operations should be self-installing, platform independent, easy to customize and amenable to using distributed bioinformatics resources available on the Internet. In order to address these issues, we present EST-PAC a web oriented multi-platform software package for expressed sequences tag (EST annotation. EST-PAC provides a solution for the administration of EST and protein sequence annotations accessible through a web interface. Three aspects of EST annotation are automated: 1 searching local or remote biological databases for sequence similarities using Blast services, 2 predicting protein coding sequence from EST data and, 3 annotating predicted protein sequences with functional domain predictions. In practice, EST-PAC integrates the BLASTALL suite, EST-Scan2 and HMMER in a relational database system accessible through a simple web interface. EST-PAC also takes advantage of the relational database to allow consistent storage, powerful queries of results and, management of the annotation process. The system allows users to customize annotation strategies and provides an open-source data-management environment for research and education in bioinformatics.

  9. Errare humanum est : sincronia, diacronia e erro

    OpenAIRE

    Teixeira, José

    1996-01-01

    O falante de uma língua natural, na maior parte dos casos, tem sempre à sua disposição mais que uma forma/fórmula. A sincronia não é um conjunto de processos unívocos (de comunicar) mas sim um sistema de identidades processuais. O que o falante faz é procurar, buscar, optar por um ou por outro. Este processo não é imóvel, fixo, estático, mas errante, sinónimo de dinâmico.

  10. Estética del rock

    OpenAIRE

    Rey, Sebastián

    2014-01-01

    El objetivo del presente artículo es el de analizar la estética del Rock en términos de la experiencia que ofrece este género musical. En primer lugar se construirá una relación entre el Nacimiento de la tragedia de Nietzsche y el surgimiento del Rock, bajo la premisa de que el origen del Rock es eminentemente dionisíaco; luego se mostrará una forma de la experiencia en la vida cotidiana de quien escucha Rock, en donde se da cuenta de la necesidad de expresar los sentimientos de placer y disp...

  11. Expressed sequence tags (ESTs) and single nucleotide ...

    African Journals Online (AJOL)

    Expressed Sequence Tags (ESTs) and Single Nucleotide Polymorphisms (SNPs) are providing in depth knowledge in plant biology, breeding and biotechnology. The emergence of many novel molecular marker techniques are changing and accelerating the process of producing mutations in plant molecular biology ...

  12. Repeats and EST analysis for new organisms

    Directory of Open Access Journals (Sweden)

    Jonassen Inge

    2008-01-01

    Full Text Available Abstract Background Repeat masking is an important step in the EST analysis pipeline. For new species, genomic knowledge is scarce and good repeat libraries are typically unavailable. In these cases it is common practice to mask against known repeats from other species (i.e., model organisms. There are few studies that investigate the effectiveness of this approach, or attempt to evaluate the different methods for identifying and masking repeats. Results Using zebrafish and medaka as example organisms, we show that accurate repeat masking is an important factor for obtaining a high quality clustering. Furthermore, we show that masking with standard repeat libraries based on curated genomic information from other species has little or no positive effect on the quality of the resulting EST clustering. Library based repeat masking which often constitutes a computational bottleneck in the EST analysis pipeline can therefore be reduced to species specific repeat libraries, or perhaps eliminated entirely. In contrast, substantially improved results can be achived by applying a repeat library derived from a partial reference clustering (e.g., from mapping sequences against a partially sequenced genome. Conclusion Of the methods explored, we find that the best EST clustering is achieved after masking with repeat libraries that are species specific. In the absence of such libraries, library-less masking gives results superior to the current practice of using cross-species, genome-based libraries.

  13. An EST database from saffron stigmas

    Directory of Open Access Journals (Sweden)

    Chiusano Maria Luisa

    2007-10-01

    Full Text Available Abstract Background Saffron (Crocus sativus L., Iridaceae flowers have been used as a spice and medicinal plant ever since the Greek-Minoan civilization. The edible part – the stigmas – are commonly considered the most expensive spice in the world and are the site of a peculiar secondary metabolism, responsible for the characteristic color and flavor of saffron. Results We produced 6,603 high quality Expressed Sequence Tags (ESTs from a saffron stigma cDNA library. This collection is accessible and searchable through the Saffron Genes database http://www.saffrongenes.org. The ESTs have been grouped into 1,893 Clusters, each corresponding to a different expressed gene, and annotated. The complete set of raw EST sequences, as well as of their electopherograms, are maintained in the database, allowing users to investigate sequence qualities and EST structural features (vector contamination, repeat regions. The saffron stigma transcriptome contains a series of interesting sequences (putative sex determination genes, lipid and carotenoid metabolism enzymes, transcription factors. Conclusion The Saffron Genes database represents the first reference collection for the genomics of Iridaceae, for the molecular biology of stigma biogenesis, as well as for the metabolic pathways underlying saffron secondary metabolism.

  14. An EST database from saffron stigmas.

    Science.gov (United States)

    D'Agostino, Nunzio; Pizzichini, Daniele; Chiusano, Maria Luisa; Giuliano, Giovanni

    2007-10-09

    Saffron (Crocus sativus L., Iridaceae) flowers have been used as a spice and medicinal plant ever since the Greek-Minoan civilization. The edible part - the stigmas - are commonly considered the most expensive spice in the world and are the site of a peculiar secondary metabolism, responsible for the characteristic color and flavor of saffron. We produced 6,603 high quality Expressed Sequence Tags (ESTs) from a saffron stigma cDNA library. This collection is accessible and searchable through the Saffron Genes database http://www.saffrongenes.org. The ESTs have been grouped into 1,893 Clusters, each corresponding to a different expressed gene, and annotated. The complete set of raw EST sequences, as well as of their electopherograms, are maintained in the database, allowing users to investigate sequence qualities and EST structural features (vector contamination, repeat regions). The saffron stigma transcriptome contains a series of interesting sequences (putative sex determination genes, lipid and carotenoid metabolism enzymes, transcription factors). The Saffron Genes database represents the first reference collection for the genomics of Iridaceae, for the molecular biology of stigma biogenesis, as well as for the metabolic pathways underlying saffron secondary metabolism.

  15. FRIEDMAN Thomas, La terre est plate

    OpenAIRE

    Kociemba, Valérie

    2008-01-01

    « L’Église dit que la terre est plate, mais j’ai vu l’ombre sur la lune et j’ai plus foi en l’ombre qu’en l’église. » (Ferdinand Magellan) Bien des siècles plus tard, Thomas Friedman réitère l’affirmation en titre de son dernier ouvrage : La terre est plate, Une brève histoire du xxie siècle. À quelle Église appartient donc ce brillant éditorialiste du « New York Times » ? À l’Église de la toute puissance des nouvelles technologies de l’information, et il n’en fait pas mystère puisqu’il décla...

  16. Bessines – Le Grand Champ Est

    OpenAIRE

    Lemaître, Séverine; Landreau, Guilhem; Ferrié, Jean-Georges; Maguer, Patrick

    2013-01-01

    Identifiant de l'opération archéologique : 204625 Date de l'opération : 2008 (SP) Plusieurs opérations de diagnostics et de fouilles ont été entreprises depuis 2003 au sud-est de la Communauté d’Agglomérations de Niort. Elles montrent une occupation protohistorique particulièrement dense de l’âge du Bronze à l’âge du Fer, au sud-est de l’ancien golfe des Pictons, notamment sur les communes adjacentes de Niort, à Aiffres et à Bessines. Suite au dépôt d’un projet de ZAC, une opération de diagno...

  17. Comparaison n'est pas raison…

    Directory of Open Access Journals (Sweden)

    Yves Guermond

    2004-11-01

    Full Text Available Dans le contexte très politisé de la question palestinienne il est difficile de séparer l’analyse scientifique des réactions passionnelles, et l’utilisation de l’apartheid comme repoussoir peut donner l’impression de dédouaner le gouvernement israélien. C’est d’ailleurs un peu semble-t-il l’intention de Frédéric Giraut quand il écrit que « l’usage radical et systématique de l’analogie vise à condamner l’Etat d’Israël et le sionisme dans leur nature même ». « Cette approche infondée » serait ,...

  18. La actualidad estética

    Directory of Open Access Journals (Sweden)

    Alfonso Hanssen

    1966-07-01

    Full Text Available "La gente que se interesa por la filosofía del arte pertenece más o menos a dos tipos: artistas con inclinación filosófica o filósofos con gusto artístico". En mayor o menor grado, la peculiaridad y limitación de la estética han tenido que ver con esta disyuntiva.

  19. Estética, Subjetividad y Conocimiento

    Directory of Open Access Journals (Sweden)

    López Avendaño, Olimpia

    2002-12-01

    Full Text Available Este artículo trata de la tradicional dicotomía conocimiento objetivo-subjetivo. En él se analiza la posición de diferentes autores en búsqueda de los elementos que conducen al nacimiento de un nuevo paradigma, integrador, que supere la antinomia y permita visualizar al conocimiento pero sobre todo al individuo que lo produce, integralmente.

  20. Amar por cartas - este inferno de amara

    Directory of Open Access Journals (Sweden)

    Matildes Demetrio dos Santos

    1999-10-01

    Full Text Available Este trabalho tem por objetivo estudar o amor nas Folhas caídas e nas Cartas de amor à Viscondessa da Luz, de Almeida Garrett.Seus poemas cheios de erotismo e sensua lidade, inspirados numa mulher casada, Rosa Montúrar Infante, escandalizaram a sociedade portuguesado sécu lo XIX. Suas cartas de amor, em segredo, criavam uma ficção de características místicas.

  1. Beleza e cirurgia estética

    OpenAIRE

    Vilas Bôas, Luana Michele da Silva

    2012-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas. Programa de Pós-Graduação em Psicologia O objetivo desse estudo foi identificar as representações sociais de homens e mulheres com diferentes formações acadêmicas a respeito de beleza e da cirurgia estética. Trata-se de um estudo de natureza descritiva e comparativa, com delineamento transversal, com a participação de 120 universitários dos cursos de Educação Física, Artes e Exatas, dis...

  2. Signaling pathways in a Citrus EST database

    Directory of Open Access Journals (Sweden)

    Angela Mehta

    2007-01-01

    Full Text Available Citrus spp. are economically important crops, which in Brazil are grown mainly in the State of São Paulo. Citrus cultures are attacked by several pathogens, causing severe yield losses. In order to better understand this culture, the Millenium Project (IAC Cordeirópolis was launched in order to sequence Citrus ESTs (expressed sequence tags from different tissues, including leaf, bark, fruit, root and flower. Plants were submitted to biotic and abiotic stresses and investigated under different development stages (adult vs. juvenile. Several cDNA libraries were constructed and the sequences obtained formed the Citrus ESTs database with almost 200,000 sequences. Searches were performed in the Citrus database to investigate the presence of different signaling pathway components. Several of the genes involved in the signaling of sugar, calcium, cytokinin, plant hormones, inositol phosphate, MAPKinase and COP9 were found in the citrus genome and are discussed in this paper. The results obtained may indicate that similar mechanisms described in other plants, such as Arabidopsis, occur in citrus. Further experimental studies must be conducted in order to understand the different signaling pathways present.

  3. AcEST(EST sequences of Adiantum capillus-veneris and their annotation) - AcEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us AcEST AcEST(EST sequences of Adiantum capillus-veneris and their annotation) Data detail Dat...a name AcEST(EST sequences of Adiantum capillus-veneris and their annotation) DOI 10.18908/lsdba.nbdc00839-0...01 Description of data contents EST sequence of Adiantum capillus-veneris and its annotation (clone ID, libr...le search URL http://togodb.biosciencedbc.jp/togodb/view/archive_acest#en Data acquisition method Capillary ...ainst UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases) Number of data entries Adiantum capillus-veneris

  4. Marketing! Onde está o Paradigma?

    Directory of Open Access Journals (Sweden)

    Deosir Flávio Lobo de Castro Júnior

    2015-09-01

    Full Text Available O debate quantitativo-qualitativo, não é uma discussão recente. O objetivo desse trabalho, portanto, é verificar por meio do conceito de paradigma, novas perspectivas de compreender as pesquisas acadêmicas em marketing, a evolução do pensamento de marketing e as metodologias utilizadas nos estudos da qualidade de serviço. Sem a pretensão de esgotar este assunto e apresentar uma conclusão final, são apresentadas pesquisas que apontam para a necessidade e a importância da pesquisa qualitativa, uma vez que auxilia ao pesquisador uma melhor compreensão da natureza complexa do mundo social em que vivemos. Segundo Santana e Gomes (2007, após análise da discussão de Hegel e Kant, concluem que a razão e a própria epistemologia são construções históricas e evoluem a partir de contradições. O presente artigo está dividido em cinco momentos. Na primeira parte apresenta-se além da introdução a constituição dos objetivos desse ensaio teórico. Na segunda parte apresenta-se uma breve discussão a respeito do conceito de paradigma e o marketing. Na terceira parte apresenta-se uma retrospectiva histórica do marketing e sua evolução de suas escolas a partir dos estudos de Miranda e Arruda (2004. Na quarta parte, são apresentadas as metodologias dos estudos sobre qualidade de serviços e finalmente na quinta parte apresenta-se as considerações finais.

  5. Crystal structure of hyperthermophilic esterase EstE1 and the relationship between its dimerization and thermostability properties

    Directory of Open Access Journals (Sweden)

    Koh Eunhee

    2007-07-01

    Full Text Available Abstract Background EstE1 is a hyperthermophilic esterase belonging to the hormone-sensitive lipase family and was originally isolated by functional screening of a metagenomic library constructed from a thermal environmental sample. Dimers and oligomers may have been evolutionally selected in thermophiles because intersubunit interactions can confer thermostability on the proteins. The molecular mechanisms of thermostabilization of this extremely thermostable esterase are not well understood due to the lack of structural information. Results Here we report for the first time the 2.1-Å resolution crystal structure of EstE1. The three-dimensional structure of EstE1 exhibits a classic α/β hydrolase fold with a central parallel-stranded beta sheet surrounded by alpha helices on both sides. The residues Ser154, Asp251, and His281 form the catalytic triad motif commonly found in other α/β hydrolases. EstE1 exists as a dimer that is formed by hydrophobic interactions and salt bridges. Circular dichroism spectroscopy and heat inactivation kinetic analysis of EstE1 mutants, which were generated by structure-based site-directed mutagenesis of amino acid residues participating in EstE1 dimerization, revealed that hydrophobic interactions through Val274 and Phe276 on the β8 strand of each monomer play a major role in the dimerization of EstE1. In contrast, the intermolecular salt bridges contribute less significantly to the dimerization and thermostability of EstE1. Conclusion Our results suggest that intermolecular hydrophobic interactions are essential for the hyperthermostability of EstE1. The molecular mechanism that allows EstE1 to endure high temperature will provide guideline for rational design of a thermostable esterase/lipase using the lipolytic enzymes showing structural similarity to EstE1.

  6. Punta del Este terrane : a better knowledge

    International Nuclear Information System (INIS)

    Preciozzi, F.; Sanchez Bettucci, L; Basei, M.

    2002-01-01

    Field Punta del Este (TPE) is constituted by a series of gneisses and migmatites formed in the range of 1000 and 900 Ma, and have been intensely reworked during Rio Doce orogeny (ca. 600-500 Ma). This cortical segment represents high-grade metamorphic terrain, correlatable with complex gneiss Southwest Africa, particularly with Kibariáno Belt - Namaqualanos recognized in the southwestern portion of Africa (Namibia). U-Pb zircon ages in tonalite granitoids indicate values ​​between 1000 and 900 Ma and were interpreted as indicating the time of generation of these rocks. This should also be the age of high-grade metamorphism that affected a large part of the gneissic rocks of the region. Moreover, the anatectic related mobilized leucosomes ages of migmatites gave ca. 520-540 Ma, indicating that the metamorphic conditions (overlapping) during the Rio Doce orogeny reached at least the amphibolite facies. The metasedimentary cover TPE occurs in the vicinity of the towns of La Paloma Rocha. These are represented by a siliciclastic metasedimentary sequence represented by Rocha Formation. Despite the poly phase deformation and low grade metamorphism affecting this training, primary structures are common such as stratification plano-parallel, cross-bedding, hummock y and massive levels with gradational stratification

  7. APOSENTADORIA: como professores vivenciam este momento?

    Directory of Open Access Journals (Sweden)

    Cristiane Nervis Conrado Machado

    2017-05-01

    Full Text Available O presente artigo buscou compreender as implicações que a aposentadoria causa na vida de professores, levando em conta o significado que a aposentadoria representa à estes, considerando os pontos de maior mudança após a aposentadoria, bem como os aspectos positivos e negativos causados pela aposentadoria na percepção dos sujeitos da pesquisa. Para tanto, foi realizada uma pesquisa qualitativa caracterizada como exploratória e descritiva com professores aposentados por mais de dois anos, com idade entre 58 e 66 anos, através de entrevistas individuais, com roteiros semiestruturados. A análise dos dados foi por meio da análise de conteúdo de Bardin (2011. Entre os principais resultados, destaca-se o significado de “liberdade” que a aposentadoria representa, a rotina como maior mudança e flexibilidade de horários dentre os pontos positivos e a falta dos alunos como aspecto negativo, contudo, pode-se perceber que a aposentadoria possibilitou e contribuiu para que os professores pudessem desfrutar de momentos de lazer, dedicando cuidado à família e realizando seus anseios e vontades.

  8. Diabète en milieu urbain de Ouagadougou au Burkina Faso: profil ...

    African Journals Online (AJOL)

    Outre ses complications métaboliques, le diabète est un facteur de risque cardiovasculaire majeur. Le but de notre étude était de ... de risque cardiovasculaire. Conclusion: il est urgent de mettre en place un programme national de lutte contre le diabète sucré et les autres maladies non transmissibles au Burkina Faso.

  9. Epaisseur de l'intima-média carotidienne et facteurs de risque ...

    African Journals Online (AJOL)

    échographie est un marqueur de risque cardio-vasculaire. L'objectif de ce travail est d'établir la corrélation entre l'épaisseur de l'intima-média carotidienne commune et les facteurs de risque cardio-vasculaire chez des sujets asymptomatiques.

  10. Kohvik-restoran C'est La Vie = Cafe-restaurant C'est La Vie

    Index Scriptorium Estoniae

    2008-01-01

    Kohvik-restorani C'est La Vie (Suur-Karja 5, Tallinn) sisekujundusest. Sisearhitekt: Maile Grünberg, kes kavandas ka valdavalt mööbli. Laevalgusti disainis Tõnis Vellama. Skulptuuride (koopiad) autor on keraamik Ülle Rajasalu. Maile Grünbergist, tema viimased tööd. Keldrikorruse ja I korruse plaan, 8 värv. vaadet, foto M. Grünbergist

  11. El nuevo regionalismo en el Este de Asia El nuevo regionalismo en el Este de Asia

    Directory of Open Access Journals (Sweden)

    Adriana Roldán Pérez

    2008-12-01

    Full Text Available Las ideas de un nuevo regionalismo surgen en el Este de Asia a finales de losaños noventa como respuesta al cambiante entorno económico mundial; estorepresenta un quiebre en la fuerte tendencia multilateralista que ha predominadohistóricamente en la región. El siguiente trabajo pretende analizar cuáles son losprincipales impedimentos para la creación de un regionalismo en el Este de Asia,qué factores están motivando un nuevo regionalismo allí y cuáles son las iniciativasregionalistas de mayor relevancia.The ideas of a new regionalism arose in East Asia in the late 90s as a response to the world’s new economic scenario. This situation sets up a framework that contrasts with the strong multilateral tendencies which have historically prevailed in the region. This work aims to analyze the obstacles for the creation of a new regionalism in East Asia, the factors motivating it, and the most relevant regionalist initiatives.

  12. EST Express: PHP/MySQL based automated annotation of ESTs from expression libraries

    Directory of Open Access Journals (Sweden)

    Pardinas Jose R

    2008-04-01

    Full Text Available Abstract Background Several biological techniques result in the acquisition of functional sets of cDNAs that must be sequenced and analyzed. The emergence of redundant databases such as UniGene and centralized annotation engines such as Entrez Gene has allowed the development of software that can analyze a great number of sequences in a matter of seconds. Results We have developed "EST Express", a suite of analytical tools that identify and annotate ESTs originating from specific mRNA populations. The software consists of a user-friendly GUI powered by PHP and MySQL that allows for online collaboration between researchers and continuity with UniGene, Entrez Gene and RefSeq. Two key features of the software include a novel, simplified Entrez Gene parser and tools to manage cDNA library sequencing projects. We have tested the software on a large data set (2,016 samples produced by subtractive hybridization. Conclusion EST Express is an open-source, cross-platform web server application that imports sequences from cDNA libraries, such as those generated through subtractive hybridization or yeast two-hybrid screens. It then provides several layers of annotation based on Entrez Gene and RefSeq to allow the user to highlight useful genes and manage cDNA library projects.

  13. EST Express: PHP/MySQL based automated annotation of ESTs from expression libraries.

    Science.gov (United States)

    Smith, Robin P; Buchser, William J; Lemmon, Marcus B; Pardinas, Jose R; Bixby, John L; Lemmon, Vance P

    2008-04-10

    Several biological techniques result in the acquisition of functional sets of cDNAs that must be sequenced and analyzed. The emergence of redundant databases such as UniGene and centralized annotation engines such as Entrez Gene has allowed the development of software that can analyze a great number of sequences in a matter of seconds. We have developed "EST Express", a suite of analytical tools that identify and annotate ESTs originating from specific mRNA populations. The software consists of a user-friendly GUI powered by PHP and MySQL that allows for online collaboration between researchers and continuity with UniGene, Entrez Gene and RefSeq. Two key features of the software include a novel, simplified Entrez Gene parser and tools to manage cDNA library sequencing projects. We have tested the software on a large data set (2,016 samples) produced by subtractive hybridization. EST Express is an open-source, cross-platform web server application that imports sequences from cDNA libraries, such as those generated through subtractive hybridization or yeast two-hybrid screens. It then provides several layers of annotation based on Entrez Gene and RefSeq to allow the user to highlight useful genes and manage cDNA library projects.

  14. Doelgroepsegmentatie in de primaire preventie van cardiovasculaire ziekten

    NARCIS (Netherlands)

    Kok, F.J.

    1982-01-01

    Profiles of target groups (target group segmentation) in the primary prevention of cardiovascular disease (CVD), can lead to an effective target group-directed approach in health education. Objectives in this thesis are: 1) To identify demographic and socioeconomic characteristics of such target

  15. Steriele handschoenen bij kleine chirurgie niet nodig

    NARCIS (Netherlands)

    M.L. Bruens (Marco); P.J. van den Berg (Pieter); R.W.M. Giard (Raimond)

    2011-01-01

    textabstractAccording to the practice guideline of the Dutch Workingparty on Infection Prevention (WIP) sterile gloves have to be worn during minor surgery by the general practitioner. This is based on the microbiological principles of Spaulding and is not supported by other evidence. Current

  16. LA CHIRURGIE ENDOSCOPIQUE BILATERALE ET SIMULTANEE ...

    African Journals Online (AJOL)

    Two patients underwent bilateral simultaneous ureteroscopy for ureteral calculi, on two others bilateral simultaneous PCNL was performed in treatment of renal lithiasis. In one patient bilateral simultaneous ureteroscopic extraction of ureteral calculi was done prior to bilateral PCNL for renal lithiasis. Ureteral stenting was ...

  17. Algunas observaciones sobre el "control del estímulo"

    OpenAIRE

    Ribes Iñesta,Emilio

    2011-01-01

    Se reflexiona sobre el concepto de control del estímulo en la teoría del condicionamiento operante y, en especial, sobre el concepto de estímulo discriminativo. Para ello se señalan los problemas lógicos y definicionales que reviste el uso del concepto de estímulo en general y el de estímulo discriminativo en particular. Se reseñan diversos experimentos del autor para mostrar la incapacidad del concepto de estímulo discriminativo para dar cuenta de una serie de datos que muestran las insufici...

  18. PCR-Based EST Mapping in Wheat (Triticum aestivum L.

    Directory of Open Access Journals (Sweden)

    J. PERRY GUSTAFSON

    2009-04-01

    Full Text Available Mapping expressed sequence tags (ESTs to hexaploid wheat is aimed to reveal the structure and function of the hexaploid wheat genome. Sixty eight ESTs representing 26 genes were mapped into all seven homologous chromosome groups of wheat (Triticum aestivum L using a polymerase chain reaction technique. The majority of the ESTs were mapped to homologous chromosome group 2, and the least were mapped to homologous chromosome group 6. Comparative analysis between the EST map from this study and the EST map based on RFLPs showed 14 genes that have been mapped by both approaches were mapped to the same arm of the same homologous chromosome, which indicated that using PCR-based ESTs was a reliable approach in mapping ESTs in hexaploid wheat.

  19. Le développement durable : est-il possible? est-il mesurable?

    Directory of Open Access Journals (Sweden)

    Belmokaddem MOSTEFA

    2011-04-01

    Full Text Available A la première question, nous répondrons bien sûr, par l’affirmative puisque tous les pays sont engagés dans une nouvelle approche du développement qui ne consiste pas seulement à produire le plus de biens et au moindre coût, afin de vendre beaucoup et réaliser de grands profits. C’était la vision en vigueur dans toutes les techniques de gestion mais on s’est rendu compte que cette production, supposée au moindre coût, était en fait, souvent d’un coût exorbitant, relativement aux dégâts occasionnés dans l’environnement et dans les nuisances occasionnées à la santé de l’homme et à sa tranquillité. Il est temps d’inclure d’autres variables dans le calcul du prix de revient tout en revoyant certaines techniques de production trop salissantes, dangereuses ou grosses consommatrices d’énergie. Nous répondrons aussi par la négative quand on pense qu’au niveau micro économique, les comportements sont édictés par les satisfactions immédiates et les maximisations des profits. Ces comportements qui ne s’intéressent qu’aux résultats à court terme, ne s’intègrent pas facilement dans les plans de développement visant les équilibres macro économiques à long terme.A la deuxième question, nous dirons qu’il y a effectivement des indicateurs pour tester les niveaux de qualité dans l’éducation, la santé, les ressources naturelles... et les gains tirés au niveau social mais les calculs économiques qui déterminent la rentabilité des projets, les profits espérés, les risques financiers encourus ne sont pas toujours en rapport avec les efforts pour réaliser le développement durable qui, eux dépassent la simple notion d’efficacité et de satisfaction. Quand on pense aux générations futures, ce n’est pas seulement en termes de durée dans le temps mais aussi dans la manière d’apprécier les gains espérés.Notre recherche repose sur ces questions qui minent bien des efforts alors qu

  20. Communication interventriculaire ischémique: à propos d'un cas observé dans le service de cardiologie du CHU-Yalgado Ouedraogo de Ouagadougou (Burkina Faso)

    Science.gov (United States)

    Yaméogo, Nobila Valentin; Ilboudo, Maurice; Seghda, Arthur; Kologo, Jonas; Millogo, Georges; Toguyéni, Boubakar Jean Yves; Samadoulougou, André; Zabsonré, Patrice

    2014-01-01

    La rupture myocardique est une complication rare mais souvent fatale de l'infarctus du myocarde aigu récent. Une patiente âgée de 72 ans, présentant une douleur thoracique typiquement angineuse évoluant depuis 34 jours, en insuffisance cardiaque globale était reçue pour une exploration cardio-vasculaire. L'examen physique retrouvait un souffle holosystolique endapexien d'intensité 3/6, irradiant en rayon de roue. La troponine T était élevée à quatre fois la normale et l'ECG objectivait une lésion sous épicardique en antéroseptoapical et une nécrose dans le même territoire. L’échodoppler cardiaque retrouvait un anévrisme septoapicolatéral avec une solution de continuité dans le segment apical du septum interventriculaire (CIV). Traitée par énoxaparine, antiagrégant plaquettaire, diurétique de l'anse, dérivés morphiniques et oxygène, la patiente présente au deuxième jour de son hospitalisation un collapsus cardio-vasculaire et décède dans un tableau de choc cardiogénique malgré l'administration des amines vasopressives à forte dose. La coronarographie n'a pu être réalisée. Ce cas illustre la gravité des complications mécaniques de l'infarctus du myocarde. L'absence de chirurgie cardiaque dans notre pays explique en grande partie l’évolution fatale de cette CIV ischémique. PMID:25922631

  1. EST data - RED | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ...st.zip File URL: ftp://ftp.biosciencedbc.jp/archive/red/LATEST/red_est.zip File size: 629 KB Simple search U...ase Database Description Download License Update History of This Database Site Policy | Contact Us EST data - RED | LSDB Archive ...

  2. Estética en prótesis removibles

    OpenAIRE

    LVM Lucas; FH Gennari; Marcello Cohello Goiato; DM Dos Santos; A Moreno; RM Falcón-Antenucci

    2010-01-01

    La estética actualmente recibe una importante consideración en el contexto social. Una sonrisa atractiva es aspiración de la mayoría de los pacientes que precisan tratamiento protésico. Los implantes propiciaron una mejoría significativa, con relación a las prótesis removibles, debido a su funcionalidad. El objetivo de este estudio fue explorar en la literatura científica moderna, a fin de hallar aspectos relacionados con la estética en la rehabilitación mediante prótesis removibles. Fueron e...

  3. Esferas estáticas en relatividad general

    Directory of Open Access Journals (Sweden)

    J. Ospino

    2007-01-01

    Full Text Available Recientemente K. Lake ha presentado un algoritmo que permit e obtener todas las soluciones estáticas de las ecuaciones de Einstein, para el caso de un fluido perfecto con simetría esférica, a partir de u na sola función dada. Este algoritmo se extiende al caso de un fluido localmen te anisótropo. Como era de esperar, este nuevo formalismo requiere del cono cimiento de dos funciones en lugar de una. Para ilustrar el método se dedu cen de nuevo algunas soluciones conocidas.

  4. Rapid EST isolation from chromosome 1R of rye

    Directory of Open Access Journals (Sweden)

    Chen Yu-Hong

    2008-03-01

    Full Text Available Abstract Background To obtain important expressed sequence tags (ESTs located on specific chromosomes is currently difficult. Construction of single-chromosome EST library could be an efficient strategy to isolate important ESTs located on specific chromosomes. In this research we developed a method to rapidly isolate ESTs from chromosome 1R of rye by combining the techniques of chromosome microdissection with hybrid specific amplification (HSA. Results Chromosome 1R was isolated by a glass needle and digested with proteinase K (PK. The DNA of chromosome 1R was amplified by two rounds of PCR using a degenerated oligonucleotide 6-MW sequence with a Sau3AI digestion site as the primer. The PCR product was digested with Sau3AI and linked with adaptor HSA1, then hybridized with the Sau3AI digested cDNA with adaptor HSA2 of rye leaves with and without salicylic acid (SA treatment, respectively. The hybridized DNA fragments were recovered by the HSA method and cloned into pMD18-T vector. The cloned inserts were released by PCR using the partial sequences in HSA1 and HSA2 as the primers and then sequenced. Of the 94 ESTs obtained and analyzed, 6 were known sequences located on rye chromosome 1R or on homologous group 1 chromosomes of wheat; all of them were highly homologous with ESTs of wheat, barley and/or other plants in Gramineae, some of which were induced by abiotic or biotic stresses. Isolated in this research were 22 ESTs with unknown functions, probably representing some new genes on rye chromosome 1R. Conclusion We developed a new method to rapidly clone chromosome-specific ESTs from chromosome 1R of rye. The information reported here should be useful for cloning and investigating the new genes found on chromosome 1R.

  5. Aproximaciones a una estética de lo americano

    Directory of Open Access Journals (Sweden)

    María del Milagro Casalla

    2010-01-01

    Full Text Available El legado filosófico que nos ha dejado Rodolfo Kusch tiene cada vez más presencia y valor en este largo itinerario de descubrirnos como americanos. La pasión de este pensador por la América profunda es una fuente de ins- piración para quienes creemos que vivir en América no es simplemente un episodio geográfico sino un horizonte desde y a partir del cual construimos nuestra identidad. En este trabajo se pretende recrear sus consideracio - nes estéticas recogidas en su ensayo “Anotaciones para una estética de lo americano”, escrito en el año 1955. Aunque se trata de uno de los primeros escritos de Rodolfo Kusch, en el mismo se encuentran presentes numerosos conceptos que luego serán tematizados extensamente a lo largo de toda su obra. Considero que el abordaje a sus consideraciones estéticas no ha sido tan frecuentado como sí otros aspectos de su pensamiento (ser-estar, geocultura, símbolo y religión, etc.. Por ello, creo que este trabajo puede constituir una orientación preeliminar para futuras interpretaciones y, a la vez, ser un humilde homenaje a su filosofía, que, a lo largo de los años, sigue estimulando a antiguas y nuevas generaciones de pensadores dedicados a la filosofía latinoamericana.

  6. Wheat EST resources for functional genomics of abiotic stress

    Directory of Open Access Journals (Sweden)

    Links Matthew G

    2006-06-01

    Full Text Available Abstract Background Wheat is an excellent species to study freezing tolerance and other abiotic stresses. However, the sequence of the wheat genome has not been completely characterized due to its complexity and large size. To circumvent this obstacle and identify genes involved in cold acclimation and associated stresses, a large scale EST sequencing approach was undertaken by the Functional Genomics of Abiotic Stress (FGAS project. Results We generated 73,521 quality-filtered ESTs from eleven cDNA libraries constructed from wheat plants exposed to various abiotic stresses and at different developmental stages. In addition, 196,041 ESTs for which tracefiles were available from the National Science Foundation wheat EST sequencing program and DuPont were also quality-filtered and used in the analysis. Clustering of the combined ESTs with d2_cluster and TGICL yielded a few large clusters containing several thousand ESTs that were refractory to routine clustering techniques. To resolve this problem, the sequence proximity and "bridges" were identified by an e-value distance graph to manually break clusters into smaller groups. Assembly of the resolved ESTs generated a 75,488 unique sequence set (31,580 contigs and 43,908 singletons/singlets. Digital expression analyses indicated that the FGAS dataset is enriched in stress-regulated genes compared to the other public datasets. Over 43% of the unique sequence set was annotated and classified into functional categories according to Gene Ontology. Conclusion We have annotated 29,556 different sequences, an almost 5-fold increase in annotated sequences compared to the available wheat public databases. Digital expression analysis combined with gene annotation helped in the identification of several pathways associated with abiotic stress. The genomic resources and knowledge developed by this project will contribute to a better understanding of the different mechanisms that govern stress tolerance in

  7. activismo y estéticas en experimento

    Directory of Open Access Journals (Sweden)

    Omar Rincón

    2005-01-01

    Full Text Available Podemos comunicarlo todo: tenemos las tecnologías de la comunicación cada vez más cerca; pero no podemos contar nada, porque no tenemos nada qué decir, ni sabemos cómo decirlo. Hay que inventarlo todo, intervenir de nuevo la sociedad desde la comunicación. Es necesario convertir los medios en experiencia estética, social y política. ¿Cómo? Usando a las tecnologías para el activismo y el experimento; recurriendo a los llamado retro, porque no es lo más actual, lo más mercado, como experiencia de inspiración y emoción. Hay que volver a las estéticas, a los modos de narrar y a las historias que están inscritas en nuestras identidades locales; en nuestras sensibilidades femeninas, juveniles o sexuales; en nuestras tradiciones étnicas, o en nuestros modos urbanos. Sólo basta una minicámara para inventar el universo. En este ensayo se realiza un alegato contra los universos estético-narrativopolíticos construidos por la máquina televisiva y la homogeneidad del mercado. En este texto se argumenta a favor del activismo comunicativo, de la exploración tecnológica y de la inspiración étnica como estrategias para generar una mayor conciencia sobre el valor social de la comunicación, la intervención estético-narrativa de la sociedad y la importancia política de los medios de la gente.

  8. Estética en prótesis removibles

    Directory of Open Access Journals (Sweden)

    LVM Lucas

    2010-06-01

    Full Text Available La estética actualmente recibe una importante consideración en el contexto social. Una sonrisa atractiva es aspiración de la mayoría de los pacientes que precisan tratamiento protésico. Los implantes propiciaron una mejoría significativa, con relación a las prótesis removibles, debido a su funcionalidad. El objetivo de este estudio fue explorar en la literatura científica moderna, a fin de hallar aspectos relacionados con la estética en la rehabilitación mediante prótesis removibles. Fueron encontrados factores importantes como: la selección de los dientes artificiales, la disposición de los mismos, la caracterización de la base de resina acrílica, además de los tipos de retenedores para las prótesis parciales removibles. La planificación y aplicación apropiada de este procedimiento, con criterios estéticos correctos, debe ser responsabilidad del profesional.

  9. El leviatán estético

    Directory of Open Access Journals (Sweden)

    Jorge Peñuela

    2011-05-01

    Full Text Available En este ensayo exploro algunos argumentos de Hannah Arendt, con el fin de mostrar las razones que explican el odio que se manifestaba en las discusiones artísticas de comienzos del siglo XX, odio que persiste aún en muchos escenarios artísticos actuales. Con Arendt sostengo que este fenómeno no está superado, porque el odio es la herramienta que despliega el Capital, para amalgamar a todos los desheredados que ha creado. Contextualizo en la práctica artística colombiana el siguiente argumento de Arendt: el capitalismo creó una amalgama de frustrados sociales, políticos y estéticos que destruye la esfera pública como lugar para el acontecer de la política, amalgama que denominó populacho. Introduzco la categoría de populacho estético, para hablar del odio que ha animado las prácticas artísticas en Colombia desde 1940.

  10. Construction of EST Database for Comparative Gene Studies of Acanthamoeba

    Science.gov (United States)

    Moon, Eun-Kyung; Kim, Joung-Ok; Xuan, Ying-Hua; Yun, Young-Sun; Kang, Se Won; Lee, Yong Seok; Ahn, Tae-In; Hong, Yeon-Chul; Chung, Dong-Il

    2009-01-01

    The genus Acanthamoeba can cause severe infections such as granulomatous amebic encephalitis and amebic keratitis in humans. However, little genomic information of Acanthamoeba has been reported. Here, we constructed Acanthamoeba expressed sequence tags (EST) database (Acanthamoeba EST DB) derived from our 4 kinds of Acanthamoeba cDNA library. The Acanthamoeba EST DB contains 3,897 EST generated from amebae under various conditions of long term in vitro culture, mouse brain passage, or encystation, and downloaded data of Acanthamoeba from National Center for Biotechnology Information (NCBI) and Taxonomically Broad EST Database (TBestDB). The almost reported cDNA/genomic sequences of Acanthamoeba provide stand alone BLAST system with nucleotide (BLAST NT) and amino acid (BLAST AA) sequence database. In BLAST results, each gene links for the significant information including sequence data, gene orthology annotations, relevant references, and a BlastX result. This is the first attempt for construction of Acanthamoeba database with genes expressed in diverse conditions. These data were integrated into a database (http://www.amoeba.or.kr). PMID:19488415

  11. Le travail social est-il de gauche ?

    OpenAIRE

    Girault, Eloïse

    2011-01-01

    Le sociographe est une revue de recherches en travail social, publiée par différents instituts de formation en travail social et qui entend proposer « un cadre de travail, d'écritures, de lectures et d'échanges pour développer une réflexion sur les méthodes et les pratiques d'intervention ». Dans ce numéro, Le sociographe s'efforce d'apporter des éléments de réponse à la question suivante : « Le travail social est-il de gauche ? ». Cette interrogation est fort légitime dans la mesure où, comm...

  12. Estética afirmativa em Arthur Schopenhauer.

    OpenAIRE

    Bastos, Eduardo Reina

    2012-01-01

    A tradição filosófica caracteriza a filosofia de Arhur Schopenhauer como pessimista como dado acabado e incontornável. Esta dissertação tem como principal objetivo demonstrar que sua parte estética se insere como uma alternativa a esta constatação e demonstrar que existe um grande contraste entre criação e negação. Através de uma reconstrução de sua teoria do conhecimento, a chamada pré-estética, e reconstrução de sua estética, nos alçamos àquela que serve como força apaziguadora da Vontade, ...

  13. Construction, database integration, and application of an Oenothera EST library.

    Science.gov (United States)

    Mrácek, Jaroslav; Greiner, Stephan; Cho, Won Kyong; Rauwolf, Uwe; Braun, Martha; Umate, Pavan; Altstätter, Johannes; Stoppel, Rhea; Mlcochová, Lada; Silber, Martina V; Volz, Stefanie M; White, Sarah; Selmeier, Renate; Rudd, Stephen; Herrmann, Reinhold G; Meurer, Jörg

    2006-09-01

    Coevolution of cellular genetic compartments is a fundamental aspect in eukaryotic genome evolution that becomes apparent in serious developmental disturbances after interspecific organelle exchanges. The genus Oenothera represents a unique, at present the only available, resource to study the role of the compartmentalized plant genome in diversification of populations and speciation processes. An integrated approach involving cDNA cloning, EST sequencing, and bioinformatic data mining was chosen using Oenothera elata with the genetic constitution nuclear genome AA with plastome type I. The Gene Ontology system grouped 1621 unique gene products into 17 different functional categories. Application of arrays generated from a selected fraction of ESTs revealed significantly differing expression profiles among closely related Oenothera species possessing the potential to generate fertile and incompatible plastid/nuclear hybrids (hybrid bleaching). Furthermore, the EST library provides a valuable source of PCR-based polymorphic molecular markers that are instrumental for genotyping and molecular mapping approaches.

  14. An optimized procedure greatly improves EST vector contamination removal

    Directory of Open Access Journals (Sweden)

    Wu Huan-Bin

    2007-11-01

    Full Text Available Abstract Background The enormous amount of sequence data available in the public domain database has been a gold mine for researchers exploring various themes in life sciences, and hence the quality of such data is of serious concern to researchers. Removal of vector contamination is one of the most significant operations to obtain accurate sequence data containing only a cDNA insert from the basecalls output by an automatic DNA sequencer. Popular bioinformatics programs to accomplish vector trimming include LUCY, cross_match and SeqClean. Results In a recent study, where the program SeqClean was used to remove vector contamination from our test set of EST data compiled through various library construction systems, however, a significant number of errors remained after preliminary trimming. These errors were later almost completely corrected by simply using a re-linearized form of the cloning vector to compare against the target ESTs. The modified trimming procedure for SeqClean was also compared with the trimming efficiency of the other two popular programs, LUCY2, and cross_match. Using SeqClean with a re-linearized form of the cloning vector significantly surpassed the other two programs in all tested conditions, while the performance of the other two programs was not influenced by the modified procedure. Vector contamination in dbEST was also investigated in this study: 2203 out of the 48212 ESTs sampled from dbEST (2007-04-18 freeze were found to match sequences in UNIVEC. Conclusion Vector contamination remains a serious concern to the data quality in the public sequence database nowadays. Based on the results presented here, we feel that our modified procedure with SeqClean should be recommended to all researchers for the task of vector removal from EST or genomic sequences.

  15. SPODOBASE : an EST database for the lepidopteran crop pest Spodoptera

    Directory of Open Access Journals (Sweden)

    Sabourault Cécile

    2006-06-01

    Full Text Available Abstract Background The Lepidoptera Spodoptera frugiperda is a pest which causes widespread economic damage on a variety of crop plants. It is also well known through its famous Sf9 cell line which is used for numerous heterologous protein productions. Species of the Spodoptera genus are used as model for pesticide resistance and to study virus host interactions. A genomic approach is now a critical step for further new developments in biology and pathology of these insects, and the results of ESTs sequencing efforts need to be structured into databases providing an integrated set of tools and informations. Description The ESTs from five independent cDNA libraries, prepared from three different S. frugiperda tissues (hemocytes, midgut and fat body and from the Sf9 cell line, are deposited in the database. These tissues were chosen because of their importance in biological processes such as immune response, development and plant/insect interaction. So far, the SPODOBASE contains 29,325 ESTs, which are cleaned and clustered into non-redundant sets (2294 clusters and 6103 singletons. The SPODOBASE is constructed in such a way that other ESTs from S. frugiperda or other species may be added. User can retrieve information using text searches, pre-formatted queries, query assistant or blast searches. Annotation is provided against NCBI, UNIPROT or Bombyx mori ESTs databases, and with GO-Slim vocabulary. Conclusion The SPODOBASE database provides integrated access to expressed sequence tags (EST from the lepidopteran insect Spodoptera frugiperda. It is a publicly available structured database with insect pest sequences which will allow identification of a number of genes and comprehensive cloning of gene families of interest for scientific community. SPODOBASE is available from URL: http://bioweb.ensam.inra.fr/spodobase

  16. Profil des Urgences Cardio-Vaculaires (UCV) au Togo a propos de ...

    African Journals Online (AJOL)

    Les AVC constituent l'essentielles es urgences cardio-vasculaires avec un taux de 78,3%. Le taux de mortalité est de 17,1% ce taux est plus enregistré entre 40 et 39 ans. Le taux de morbidité est de 64,1%. Nous retiendrons que les urgences cardio-vasculaires sont très fréquentes au Togo et touche une population très ...

  17. Análisis estático de programas

    OpenAIRE

    Arroyo, Marcelo; Bavera, Francisco; Regis, Germán

    2010-01-01

    En el presente trabajo se describen las líneas de investigación en desarrollo por el grupo de investigación en análisis estático de propiedades de programas. Los integrantes del grupo atacan el problema del análisis de propiedades de programas mediante diferentes enfoques. Algunos trabajan a nivel de modelos mientras que otros lo hacen directamente a nivel de código fuente. Se utilizan técnicas tanto de análisis estático liviano como pesados (basados en demostradores de teoremas o model-ch...

  18. Estéticas de las culturas de ocio

    OpenAIRE

    Sañudo Vélez, Luis Guillermo

    2009-01-01

    El presente trabajo tiene como propósito explorar el significado estético del ocio en la cultura contemporánea. Para cumplir este objetivo ha sido necesario hacer un breve balance de la manera como el concepto de ocio se ha entendido tanto en los escritos de algunos destacados filósofos (Aristóteles, Séneca y Kant) como en los escritos de algunos pensadores sociales modernos (Thorstein Veblen) y contemporáneos (Jean Baudrillard, Erich Weber, Joffre Dumazedier, Gilles Lipovetsky, Vicente Verdú...

  19. Dia mundial da espirometria na ESTeSL

    OpenAIRE

    Dias, Hermínia Brites; Carolino, Elisabete

    2011-01-01

    Objectivos do estudo: associar a ESTeSL e a AC de CPL às comemorações do Dia Mundial da Espirometria promovidas pela Fundação Europeia do Pulmão; realizar o maior número possível de espirometrias; consciencializar a comunidade para o papel da espirometria no diagnóstico precoce da patologia respiratória; colocar a ESTeSL, enquanto entidade formadora de Técnicos de Cardiopneumologia, na primeira linha das iniciativas relacionadas com o estudo espirométrico; identificar possíveis alter...

  20. Evidencias visuales de las nuevas estéticas mestizas

    OpenAIRE

    Andrade Polo, Pedro Gustavo

    2017-01-01

    El presente trabajo, pretende reflexionar y evidenciar, la visualidad que ha tenido la estética de la imagen latinoamericana en su proceso de mestizaje, concentrándose en aquella identidad fragmentaria y poco codificada que se da en los espacios locales y populares .Planteo entonces una actitud que se presenta emancipada de una estética formal. Despojada de la carga que históricamente ha impuesto la “identidad” latinoamericana, se instala libre e irresponsable para crear sus íconos y definici...

  1. Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean (Ricinus communis L.

    Directory of Open Access Journals (Sweden)

    Yang Jun-Bo

    2010-12-01

    Full Text Available Abstract Background The castor bean (Ricinus communis L., a monotypic species in the spurge family (Euphorbiaceae, 2n = 20, is an important non-edible oilseed crop widely cultivated in tropical, sub-tropical and temperate countries for its high economic value. Because of the high level of ricinoleic acid (over 85% in its seed oil, the castor bean seed derivatives are often used in aviation oil, lubricants, nylon, dyes, inks, soaps, adhesive and biodiesel. Due to lack of efficient molecular markers, little is known about the population genetic diversity and the genetic relationships among castor bean germplasm. Efficient and robust molecular markers are increasingly needed for breeding and improving varieties in castor bean. The advent of modern genomics has produced large amounts of publicly available DNA sequence data. In particular, expressed sequence tags (ESTs provide valuable resources to develop gene-associated SSR markers. Results In total, 18,928 publicly available non-redundant castor bean EST sequences, representing approximately 17.03 Mb, were evaluated and 7732 SSR sites in 5,122 ESTs were identified by data mining. Castor bean exhibited considerably high frequency of EST-SSRs. We developed and characterized 118 polymorphic EST-SSR markers from 379 primer pairs flanking repeats by screening 24 castor bean samples collected from different countries. A total of 350 alleles were identified from 118 polymorphic SSR loci, ranging from 2-6 per locus (A with an average of 2.97. The EST-SSR markers developed displayed moderate gene diversity (He with an average of 0.41. Genetic relationships among 24 germplasms were investigated using the genotypes of 350 alleles, showing geographic pattern of genotypes across genetic diversity centers of castor bean. Conclusion Castor bean EST sequences exhibited considerably high frequency of SSR sites, and were rich resources for developing EST-SSR markers. These EST-SSR markers would be particularly

  2. A estética como factor promotor da aprendizagem

    Directory of Open Access Journals (Sweden)

    Fátima Maria BEZERRA BARBOSA

    2012-02-01

    Full Text Available La reflexión filosófica sobre el arte, lo hermoso, lo bueno, lo verdadero y su capacidad de promover el desarrollo humano es un tema que remonta a Platón en la cultura occidental. Considerando que la educación debe ser entendida como un fenómeno integral, que abarca no sólo las dimensiones intelectuales sino también las dimensiones sensibles, la intuición y el raciocinio, las capacidades imaginativas y creativas, el «saber ser» y el «saber estar» parejas del «conocer» y del «saber hacer», pensamos que es incuestionable la importancia de la estética en el fenómeno educativo. El objetivo de este trabajo es demostrar la importancia de la educación estética como motor fundamental de desarrollo humano y, en este sentido, como catalizador de la motivación y del aprendizaje, para que, a través de la imaginación y de la creatividad, sea posible la innovación en la construcción de vías alternativas para un mundo más humano y placentero.

  3. Releyendo Frankfurt: la Experiencia Estética en Adorno

    Directory of Open Access Journals (Sweden)

    Stra, Sebastián Matías

    2013-01-01

    Full Text Available [es]Intentaremos en este trabajo explorar la noción de Experiencia Estética en la obra de Theodor Adorno, entendiéndola como una compleja mediación conceptual que nos puede permitir dar cuenta de las oscuras configuraciones de la relación entre arte, cultura y conocimiento en la producción del pensador de Frankfurt. En este marco, cruzaremos las reflexiones estéticas adornianas con lecturas más cercanas a nuestro campo disciplinar, como la noción de Industria Cultural y la interpretación del mito y su derivación en la conformación de la racionalidad moderna. [en] In this work, we will try to explore the notion of Aesthetic Experience in Theodor Adorno`s work, understanding it as a complex conceptual mediation that can allow us getting in the dark settings of the relationship between art, culture and knowledge in the production of Frankfurt`s thinker. Like this, we are going to cross adornians aesthetic reflections with readings that are going to be closer to our disciplinary field, as the notion of cultural industry and the interpretation of myth and its derivation in the configuration of modern rationality.

  4. Estética y espiritualidad. [RESEÑA

    OpenAIRE

    Caballero, J.L. (Juan Luis)

    2014-01-01

    Francisco Javier SANCHO FERMÍN (coord.), Estética y espiritualidad. «Via pulchritudinis». La belleza en el arte sagrado, la educación, la música, la arquitectura, el cine, la pintura, Burgos: Monte Carmelo – Ávila: CITeSUniversidad de la Mística, 2012, 31

  5. “Quem Está Falando?”

    Directory of Open Access Journals (Sweden)

    Rôssi Alves Gonçalves

    2016-07-01

    comunitários, a princípio apadrinhados por ilustres nomes da cultura brasileira, vêm pontuando o meio cultural com obras, no mínimo, provocativas e que desnudam questões que já pareciam esgotadas. Com isso, estão conseguindo movimentar o tradicional cânone literário brasileiro.

  6. Ambientes virtuais e redes: estética e socialidade

    Directory of Open Access Journals (Sweden)

    Daniela Stevanin Hoffmann

    2011-12-01

    Full Text Available Este artigo propõe-se a apresentar dois ambientes virtuais, o AMADIS e o Orkut, a fim de estabelecer relações entre eles, tendo como apoio teórico os conceitos de estética e socialidade. O primeiro é um ambiente virtual de aprendizagem desenvolvido para fins pedagógicos; o segundo é um ambiente virtual que tem alcance mundial e agrega indivíduos por suas redes de interesses e amizades. O objetivo deste artigo é analisar o entrelaçamento entre teoria e algumas das características dos ambientes, suas semelhanças e distinções, a partir do estudo da formação de redes digitais suscitadas por influências estéticas. Foi realizado um estudo qualitativo comparativo estrutural entre os ambientes, colocando-os em paralelo, traçando analogias entre suas áreas, funcionalidades e possibilidades de ações dos usuários. Ao final do estudo e da argumentação, conclui-se que as redes de relações entre os conceitos-chave do estudo da correlação estética-socialidade (neotribalismo, corpo coletivo, ator-persona, espacialidade e pertença, de acordo com Maffesoli, estruturadas a partir do confronto entre as subdivisões dos ambientes, são rearranjáveis e recriáveis.

  7. GrowInclusive : la plateforme tant attendue est en construction ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    4 janv. 2018 ... La croissance inclusive est devenue une idée centrale dans la plupart des plans de développement nationaux, mais pour beaucoup, elle reste avant tout une aspiration. Les gouvernements, les bailleurs de fonds internationaux et les organisations de la société civile manquent de mécanismes éprouvés ...

  8. Novel expressed sequence tag- simple sequence repeats (EST ...

    African Journals Online (AJOL)

    Using different bioinformatic criteria, the SUCEST database was used to mine for simple sequence repeat (SSR) markers. Among 42,189 clusters, 1,425 expressed sequence tag- simple sequence repeats (EST-SSRs) were identified in silico. Trinucleotide repeats were the most abundant SSRs detected. Of 212 primer pairs ...

  9. EST Table: DN236912 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN236912 EST00037 10/09/29 79 %/179 aa ref|NP_001119732.1| lebocin-3 precursor [Bom...byx mori] sp|P55796.2|LEB3_BOMMO RecName: Full=Lebocin-3; Short=LEB 3; Flags: Precursor dbj|BAA22883.1| leboc

  10. Las afecciones estéticas: un problema para prevenir

    Directory of Open Access Journals (Sweden)

    Yulisa Otero Baxter

    2001-08-01

    Full Text Available La estética facial asociada con los dientes ha preocupado a la sociedad desde la antigüedad. El temor al rechazo social por cambios de forma, color o posiciones dentales, puede producir un gran impacto psicológico en los pacientes. Con el objetivo de valorar la repercusión psicosocial de las alteraciones estéticas faciales, de determinar el nivel de prioridad que ocupan los tratamientos estéticos dentales y demostrar así la necesidad de intensificar nuestra labor educativa encaminada a evitar aquellas afecciones estéticas que más inciden en nuestra población, procedimos a la aplicación de una encuesta anónima en 110 pacientes que acudieron al Servicio de Estomatología Integral de la Facultad de Estomatología entre noviembre de 1999 y abril del 2000. Se recogió información sobre: actitud asumida por pacientes afectados, prioridades de tratamientos, etcétera. Dicha información fue procesada en tablas estadísticas. Los resultados del trabajo nos permiten concluir que las afecciones estéticas dentales tienen gran repercusión psicosocial, lo que unido al haber encontrado las lesiones traumáticas como las afecciones estéticas de mayor prevalencia, nos evidencia la necesidad de enfatizar nuestra labor educativa en este aspecto.Society has worried about facial esthetics associated with teeth since ancient times. Being afraid of social rejection due to changes of dental form, color or positions may produce a great psychological impact on patients. In order to assess the psychosocial repercussion of the facial esthetic alterations, to determine the priorty level of the esthetic dental treatments and to show the need of intensifying our educative work directed to prevent the most common esthetic affections in our population, we did an anonimous survey among 110 patients that received attention at the Service of Comprehensive Stomatology of the Faculty of Stomatology between November, 1999, and April, 2000. Information was gathered

  11. ¿Está en peligro el periodismo?

    Directory of Open Access Journals (Sweden)

    José Rodríguez Elizondo

    2012-07-01

    Full Text Available Debatir sobre este tema tiene una historia propia. En un artículo publicado el año 2000 me referí al futuro de la prensa, las nuevas tecnologías de la comunicación y la hiper y auto-información como eventuales amenazas. El tema, de apariencia imponente y con hipótesis ominosas, tuvo de mi parte una decodificación optimista. Dado que aquel futuro lleva ya más de una década de desarrollo, este nuevo artículo servirá para contrastar las percepciones y los pronósticos de entonces. Lo haré en el disfrute de la mayor libertad crítica que es posible concebir, que es la que se expresa como autocrítica.

  12. Sobre a estética platônica

    Directory of Open Access Journals (Sweden)

    Luis Felipe Bellintani Ribeiro

    2007-04-01

    Full Text Available O texto começa pela consideração da aparente posição desfavorável da estética, entendida tanto como o domínio da arte, quanto como o relativo à sensação, perante a metafísica platônica. A consideração posterior, porém, do caráter ontológico do critério metafísico, que, em tese, poria a arte e a sensação a seu reboque, ensejou, mais que uma reabilitação, uma inversão: a metafísica platônica é que seria fundada numa estética.

  13. Pedro Almodovara filmu stils un estētika

    OpenAIRE

    Majorenkova, Madara

    2013-01-01

    Šī darba nosaukums ir „Pedro Almodovara filmu stils un estētika”. Darba galvenais mērķis ir pierādīt, ka P.Almodovaram piemīt savs stils un estētika. Almodovara karjeras uzplaukums sākās “La Movida Madrileña” laikā. “La Movida Madrileña” ir sociokulturāla kustība, kura parādījās pirmajos pārejas gados pēc Franko režīma Spānijā. Šī laikmeta kino atainoja visu to, kas bija aizliegts Franko režīma laikā, piemēram, homoseksualitāte, narkotiku lietošana, prostitūtica, transseksualitāte. Dar...

  14. ¿Está escuchando? ASP (:60)

    Centers for Disease Control (CDC) Podcasts

    2015-09-08

    Este anuncio de servicio público de radio motivan a las mujeres a aprender los síntomas del cáncer ginecológico y a prestar atención a lo que les dice su cuerpo.  Created: 9/8/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 9/8/2015.

  15. ¿Está escuchando? ASP (:30)

    Centers for Disease Control (CDC) Podcasts

    2015-09-08

    Este anuncio de servicio público de radio motivan a las mujeres a aprender los síntomas del cáncer ginecológico y a prestar atención a lo que les dice su cuerpo.  Created: 9/8/2015 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 9/8/2015.

  16. Extra heavy crudes and bitumens in the new EST technology

    International Nuclear Information System (INIS)

    Montanari, R.

    2001-01-01

    Strategic and market reasons dictate for the diversification of the supply sources. The huge reserves of extra heavy crudes and oil sands bitumen will provide the answer in this direction, but it is mandatory the availability of a suitable technology to process these poor quality feedstocks. EST Technology, developed by Snamprogetti and EniTecnologie, allows to get full conversion to produce high quality distillates [it

  17. « Le Paradis, c’est les autres »

    Directory of Open Access Journals (Sweden)

    Rachid Amirou

    2008-11-01

    Full Text Available L’engouement pour des sites ou des lieux est souvent dû à des « Nous » (des communautés éphémères, qui se constituent et qui consacrent ainsi l’espace de visite. La consécration collective est souvent un préalable au succès de certaines destinations (Ibiza, Saint-Tropez, Marrakech, etc.. Le succès des formules de villages de vacances hermétiquement fermés à l’extérieur et indifférents au pays de séjour atteste de l’existence d’un état d’esprit qu’on a classé sous le terme générique de « bulle touristique ». Cependant, la constitution de ces « bulles » – c’est-à-dire la rencontre d’un imaginaire, d’un « nous » et d���un espace touristiques – ne se fait pas uniquement par l’exclusion de l’autre. Elle repose aussi sur un principe interne positif : le rêve d’une sociabilité parfaite, une sorte de paradis relationnel. Le « paradis » touristique, c’est d’abord une rencontre de plain-pied avec les autres (« l’ambiance », souvent d’ailleurs avec les mêmes – rencontre synonyme de relations différentes et moins stressantes, basées sur une connivence réelle ou fantasmée. La généalogie de ce paradis touristique relationnel, qui peut être aussi un enfer pour les autres, reste à décrire et à expliciter, en montrant comment il compose le noyau central de l’imaginaire touristique.

  18. MELOGEN: an EST database for melon functional genomics.

    Science.gov (United States)

    Gonzalez-Ibeas, Daniel; Blanca, José; Roig, Cristina; González-To, Mireia; Picó, Belén; Truniger, Verónica; Gómez, Pedro; Deleu, Wim; Caño-Delgado, Ana; Arús, Pere; Nuez, Fernando; Garcia-Mas, Jordi; Puigdomènech, Pere; Aranda, Miguel A

    2007-09-03

    Melon (Cucumis melo L.) is one of the most important fleshy fruits for fresh consumption. Despite this, few genomic resources exist for this species. To facilitate the discovery of genes involved in essential traits, such as fruit development, fruit maturation and disease resistance, and to speed up the process of breeding new and better adapted melon varieties, we have produced a large collection of expressed sequence tags (ESTs) from eight normalized cDNA libraries from different tissues in different physiological conditions. We determined over 30,000 ESTs that were clustered into 16,637 non-redundant sequences or unigenes, comprising 6,023 tentative consensus sequences (contigs) and 10,614 unclustered sequences (singletons). Many potential molecular markers were identified in the melon dataset: 1,052 potential simple sequence repeats (SSRs) and 356 single nucleotide polymorphisms (SNPs) were found. Sixty-nine percent of the melon unigenes showed a significant similarity with proteins in databases. Functional classification of the unigenes was carried out following the Gene Ontology scheme. In total, 9,402 unigenes were mapped to one or more ontology. Remarkably, the distributions of melon and Arabidopsis unigenes followed similar tendencies, suggesting that the melon dataset is representative of the whole melon transcriptome. Bioinformatic analyses primarily focused on potential precursors of melon micro RNAs (miRNAs) in the melon dataset, but many other genes potentially controlling disease resistance and fruit quality traits were also identified. Patterns of transcript accumulation were characterised by Real-Time-qPCR for 20 of these genes. The collection of ESTs characterised here represents a substantial increase on the genetic information available for melon. A database (MELOGEN) which contains all EST sequences, contig images and several tools for analysis and data mining has been created. This set of sequences constitutes also the basis for an oligo

  19. CONQuEST - Menu-selectable database system

    Energy Technology Data Exchange (ETDEWEB)

    Yeko, J.D. (Illinois State Geological Survey, Champaign (USA))

    1989-08-01

    The well database unit of the Illinois State Geological Survey Oil and Gas section began to design and develop a technically advanced oil and gas database system in 1988. The CONQuEST system integrates and replaces the existing oil and gas, water, coal, and geotechnical database systems. CONQuEST uses a distributed relational data model that allows integrated storage and retrieval of different data and well types in an almost unlimited variety of report forms. The software, written in C, consists of five menu-selectable modules that allow a novice computer user to enter, edit, retrieve, and report data. The GeoDES module is used to enter data from paper records and consists of numerous fill-in-the-blank screens. The TIDE module is used to edit or delete any existing data. There are two modules for data retrieval. QuARTz is used for quick, preplanned retrievals and ToPAz is used for self-designed retrievals. ToPAz retrieval designs may be saved and added to the menu systems and then accessed through the QuARTz module. The COReS module consists of numerous predesigned report options. Standard Query Language (SQL) is also available as an option. CONQuEST is currently run on a Digital Equipment Corporation VAX, that is interfaced to a series of PCs; however, all software can be run on a PC only. Benefits of the CONQuEST system over the previous system are increased speed, greater flexibility, the ability to run on a PC, and the menu system that allows for successful access and use of the data by novice computer users. Data system use is available to the general public for a fee.

  20. MELOGEN: an EST database for melon functional genomics

    Directory of Open Access Journals (Sweden)

    Puigdomènech Pere

    2007-09-01

    Full Text Available Abstract Background Melon (Cucumis melo L. is one of the most important fleshy fruits for fresh consumption. Despite this, few genomic resources exist for this species. To facilitate the discovery of genes involved in essential traits, such as fruit development, fruit maturation and disease resistance, and to speed up the process of breeding new and better adapted melon varieties, we have produced a large collection of expressed sequence tags (ESTs from eight normalized cDNA libraries from different tissues in different physiological conditions. Results We determined over 30,000 ESTs that were clustered into 16,637 non-redundant sequences or unigenes, comprising 6,023 tentative consensus sequences (contigs and 10,614 unclustered sequences (singletons. Many potential molecular markers were identified in the melon dataset: 1,052 potential simple sequence repeats (SSRs and 356 single nucleotide polymorphisms (SNPs were found. Sixty-nine percent of the melon unigenes showed a significant similarity with proteins in databases. Functional classification of the unigenes was carried out following the Gene Ontology scheme. In total, 9,402 unigenes were mapped to one or more ontology. Remarkably, the distributions of melon and Arabidopsis unigenes followed similar tendencies, suggesting that the melon dataset is representative of the whole melon transcriptome. Bioinformatic analyses primarily focused on potential precursors of melon micro RNAs (miRNAs in the melon dataset, but many other genes potentially controlling disease resistance and fruit quality traits were also identified. Patterns of transcript accumulation were characterised by Real-Time-qPCR for 20 of these genes. Conclusion The collection of ESTs characterised here represents a substantial increase on the genetic information available for melon. A database (MELOGEN which contains all EST sequences, contig images and several tools for analysis and data mining has been created. This set of

  1. Multinaturalismo y estéticas de alteridad

    Directory of Open Access Journals (Sweden)

    Mario Madroñero Morillo

    2012-09-01

    Full Text Available La propuesta de una estética de alteridad problematiza la concepción de representación y su historia. Los conceptos de mimesis, formación y figuración serán aquí removidos del lugar habitual que ocupan en la teoría estética y la práctica artística, buscando una etno-grafía y estética de alteridad que permita una aproximación y ahondamiento de una relación vital con la alteridad de la tierra. Tal relación se aborda desde las márgenes que permiten los conceptos de la antropología pos-estructural propuestos por Eduardo Viveiros de Castro,multinaturalismo y perspectivismo, referidos a experiencias chamánicas y del pensar amerindio, como trazo y rasgo de modos de ser diferentes, de “procesos de alteración diferencial”, que tendrán en algunas acciones artísticas su exposición y donación de sentido.

  2. Professor-psicopedagogo: o que este profissional faz na escola

    Directory of Open Access Journals (Sweden)

    Caroline Andrea Pottker

    Full Text Available Este estudo teve como objetivo identificar o porquê da presença do professor-psicopedagogo nas escolas e qual a função que este desempenha dentro delas, bem como, suas implicações no processo ensino-aprendizagem. Para tanto, foram entrevistados dez professores-psicopedagogos, pertencentes a cinco escolas localizadas no Estado do Paraná. Os resultados apontaram que o cargo de professor-psicopedagogo foi ocupado por professores que tinham o título de especialista em Psicopedagogia, os quais passaram a realizar avaliações psicopedagógicas dos alunos com dificuldades de aprendizagem. Segundo a maioria desses profissionais, uma de suas funções constitui-se em auxiliar o professor que apresenta em sala de aula alunos com dificuldades de aprendizagem, assim como tentar compreender o porquê de estes alunos não estarem aprendendo, sobretudo, ajudá-los na reversão desta problemática. Concluímos que a atuação deste profissional nestas escolas pouco tem contribuído para resolução das dificuldades de aprendizagem, pois centram-se no indivíduo e não no processo ensino-aprendizagem.

  3. Gene expression profiling of chicken primordial germ cell ESTs

    Directory of Open Access Journals (Sweden)

    Lim Dajeong

    2006-08-01

    Full Text Available Abstract Background Germ cells are the only cell type that can penetrate from one generation to next generation. At the early embryonic developmental stages, germ cells originally stem from primordial germ cells, and finally differentiate into functional gametes, sperm in male or oocyte in female, after sexual maturity. This study was conducted to investigate a large-scale expressed sequence tag (EST analysis in chicken PGCs and compare the expression of the PGC ESTs with that of embryonic gonad. Results We constructed 10,851 ESTs from a chicken cDNA library of a collection of highly separated embryonic PGCs. After chimeric and problematic sequences were filtered out using the chicken genomic sequences, there were 5,093 resulting unique sequences consisting of 156 contigs and 4,937 singlets. Pearson chi-square tests of gene ontology terms in the 2nd level between PGC and embryonic gonad set showed no significance. However, digital gene expression profiling using the Audic's test showed that there were 2 genes expressed significantly with higher number of transcripts in PGCs compared with the embryonic gonads set. On the other hand, 17 genes in embryonic gonads were up-regulated higher than those in the PGC set. Conclusion Our results in this study contribute to knowledge of mining novel transcripts and genes involved in germline cell proliferation and differentiation at the early embryonic stages.

  4. Analysis of Health Data from Ten Years of Polaris Submarine Patrols

    Science.gov (United States)

    1979-01-01

    en revanche, ceux-la ont signale moiro de maladies - genito-urinaires, systemiques (infections et mononucleose ci-inclus), craniennes, et...classifications suivantes est diminuee: respiratoire, otorhinolaryngologique, gastro-tntestinaux, cardiovasculaire ,. urologique, et general (qui comprend

  5. Cholécystectomie laparoscopique ambulatoire: première expérience en Tunisie

    OpenAIRE

    Zaafouri, Haithem; Mrad, Skander; Khedhiri, Nizar; Haddad, Dhafer; Bouhafa, Ahmed; Maamer, Anis Ben

    2017-01-01

    Résumé Introduction La cholécystectomie laparoscopique est le gold standard de la prise en charge des calculs vésiculaires symptomatiques. Il existe une importante controverse quant au fait de savoir si elle devrait être pratiquée en chirurgie ambulatoire ou dans le cadre d'une chirurgie avec hospitalisation d'une nuit pour ce qui concerne la sécurité des patients. Le but du travail est d’évaluer l'impact de la cholécystectomie laparoscopique en chirurgie ambulatoire versus en chirurgie avec ...

  6. Hora do jogo: os professores estão prontos?

    Directory of Open Access Journals (Sweden)

    Eliana Maria Magnani

    2018-04-01

      Hora del juego: ¿los profesores están preparados? RESUMEN Las ideas discutidas en este texto están relacionadas con las actividades de enseñanza, investigación y extensión desarrolladas desde 2012 a través de la Universidad Estadual de Oeste de Paraná – UNIOESTE. El artículo señala la importancia de la clasificación y catalogación de juegos educativos, explicitando qué es un importante conocimiento propuesto a los Centros Municipales de Educación Infantil (CMEIs, localizados en una ciudad del Suroeste de Paraná, CMEIs que, en función de una ley local, empezaron la (re implementación de ludotecas en 2007. La metodología se efectuó en fases, dos de dichas fases fueron significativas para la revisión de la implementación, a saber: diagnóstico de cómo se organizaban los materiales existentes en las ludotecas, curso sobre la importancia de la clasificación y catalogación de diferentes tipos de juegos. Para ello, toda revisión se apoyó en las ideas de Jean Piaget (2010/1946, 1994/1932, quien elaboró una teoría sobre el juego. Dichos conocimientos sirvieron para la comprensión de que los maestros no están preparados para este tipo de actividad didáctico-pedagógica, pues dicha educación es un proceso que demanda toma de conciencia de propia praxis, la cual requiere el (re aprendizaje a respecto de los objetivos de diferentes tipos de juegos y del desarrollo infantil, siendo que ello necesita ocurrir en la formación inicial y en la formación continua de profesores. Por consiguiente, es necesaria la involucración de los (futuros profesores en actividades lúdicas de enseñanza, investigación y extensión realizadas en universidades. Palabras clave: Clasificación de Juegos. Educación Infantil. Formación docente.

  7. A salmonid EST genomic study: genes, duplications, phylogeny and microarrays

    Directory of Open Access Journals (Sweden)

    Brahmbhatt Sonal

    2008-11-01

    Full Text Available Abstract Background Salmonids are of interest because of their relatively recent genome duplication, and their extensive use in wild fisheries and aquaculture. A comprehensive gene list and a comparison of genes in some of the different species provide valuable genomic information for one of the most widely studied groups of fish. Results 298,304 expressed sequence tags (ESTs from Atlantic salmon (69% of the total, 11,664 chinook, 10,813 sockeye, 10,051 brook trout, 10,975 grayling, 8,630 lake whitefish, and 3,624 northern pike ESTs were obtained in this study and have been deposited into the public databases. Contigs were built and putative full-length Atlantic salmon clones have been identified. A database containing ESTs, assemblies, consensus sequences, open reading frames, gene predictions and putative annotation is available. The overall similarity between Atlantic salmon ESTs and those of rainbow trout, chinook, sockeye, brook trout, grayling, lake whitefish, northern pike and rainbow smelt is 93.4, 94.2, 94.6, 94.4, 92.5, 91.7, 89.6, and 86.2% respectively. An analysis of 78 transcript sets show Salmo as a sister group to Oncorhynchus and Salvelinus within Salmoninae, and Thymallinae as a sister group to Salmoninae and Coregoninae within Salmonidae. Extensive gene duplication is consistent with a genome duplication in the common ancestor of salmonids. Using all of the available EST data, a new expanded salmonid cDNA microarray of 32,000 features was created. Cross-species hybridizations to this cDNA microarray indicate that this resource will be useful for studies of all 68 salmonid species. Conclusion An extensive collection and analysis of salmonid RNA putative transcripts indicate that Pacific salmon, Atlantic salmon and charr are 94–96% similar while the more distant whitefish, grayling, pike and smelt are 93, 92, 89 and 86% similar to salmon. The salmonid transcriptome reveals a complex history of gene duplication that is

  8. Kyste hydatique du rein chez l’enfant: à propos de 8 cas

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    A. Ksia

    2014-12-01

    Conclusion: Le traitement du kyste hydatique du rein chez l’enfant est essentiellement chirurgical et en règle conservateur. Il peut être mené par chirurgie à ciel ouvert ou par chirurgie vidéo-assistée qui a l’avantage d’être mini-invasive. Le pronostic est fréquemment favorable.

  9. War Medicine and Surgery, Punishment and Torture at the time of the Regiments of Renown - Chirurgie de guerre, médecine militaire, punitions et torture à l’époque des compagnies de mercenaires - Medicina e Chirurgia da guerra, Punizioni e Tortura all’epoca delle Compagnie di Ventura

    Directory of Open Access Journals (Sweden)

    Lugaresi M.

    2009-08-01

    Full Text Available This article highlights the main surgical and medical issues related to the age of the Regiments of Renown. Analyzing the main causes of death of Condottieres and Captains in the period 1300-1580, the treatment of wounds and the development of skills involved in the surgery of war, the more frequent Italian epidemic diseases in that time, the punishments and torture in force in the Republic of Venice, are described.Cet article expose les aspects les plus importants en matière de médecine et de chirurgie à l'époque des compagnies de mercenaires. En analysant les principales causes de décès des condottières et des capitaines entre 1300 et 1580, l’autrice décrit le traitement des blessures et le développement des techniques chirurgicales de guerre, les épidémies les plus fréquentes en Italie à cette époque-là, les peines et les tortures en vigueur sous la République Sérénissime de Venise.Questo articolo illustra i principali aspetti medici e chirurgici connessi all’epoca delle Compagnie di Ventura. Analizzando le principali cause di decesso dei Condottieri e dei Capitani nel periodo 1300-1580, vengono descritte la cura delle ferite e lo sviluppo delle tecniche applicate nella chirurgia da guerra, le epidemie più frequenti che colpirono l’Italia in tale epoca, le pene e la tortura in vigore nella Serenissima Repubblica di Venezia.

  10. Esplenectomía incidental: ¿Está justificada?

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    Eddy Sierra Enrique

    1997-12-01

    Full Text Available Se revisaron 77 historias clínicas de pacientes que presentaban lesiones incidentales del bazo ocurridas entre 1985 y 1994, durante operaciones intraabdominales y por examen laparoscópico. Las funciones inmunológicas del bazo y su papel contra las infecciones están bien definidas, por lo cual al ser suprimido este órgano a consecuencia de lesiones incidentales, favorecen las infecciones en dichos pacientes de por vida. De las 77 lesiones incidentales se realizaron 47(61 % esplenectomías incidentales y 30(39 % cirugías conservadoras. La úlcera duodenal y la hernia hiatal son las causas principales de esplenectomía incidental. Se presentaron 11 infecciones (23,4 % y 1 fallecido (2,1 % en la esplenectomía incidental contra 1 infección (3,3 % y ningún fallecido en la cirugía conservadora. Se llega a la conclusión de que la esplenectomía no está justificada dadas las desventajas que presenta77 medical histories of patients who presented incidental injuries of the spleen occurred between 1985 and 1994, during intraabdominal operations and by laparoscopic examination, were reviewed. The immunological functions of the spleen and its role against infections are well defined, so the removal of this organ due to incidental injuries favors the appearance of infections in these patients for the rest of their lives. Of the 77 incidental injuries 47 (61 % incidental splenectomies and 30 (39 % conservative surgeries were performed. Duodenal ulcer and hiatal hernia proved to be the main causes of incidental splenectomy. There were 11 infections (23.4 % and 1 death (2.1 % in the incidental splenectomy compared with 1 infection (3.3 % and no death in the conservative surgery. It is concluded that splenectomy is not justified because of its disadvantages

  11. Distribution of DNA repair-related ESTs in sugarcane

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    W.C. Lima

    2001-12-01

    Full Text Available DNA repair pathways are necessary to maintain the proper genomic stability and ensure the survival of the organism, protecting it against the damaging effects of endogenous and exogenous agents. In this work, we made an analysis of the expression patterns of DNA repair-related genes in sugarcane, by determining the EST (expressed sequence tags distribution in the different cDNA libraries of the SUCEST transcriptome project. Three different pathways - photoreactivation, base excision repair and nucleotide excision repair - were investigated by employing known DNA repair proteins as probes to identify homologous ESTs in sugarcane, by means of computer similarity search. The results showed that DNA repair genes may have differential expressions in tissues, depending on the pathway studied. These in silico data provide important clues on the potential variation of gene expression, to be confirmed by direct biochemical analysis.As vias de reparo de DNA são requeridas para manter a necessária estabilidade genômica e garantir a sobrevivência do organismo, frente aos efeitos deletérios causados por fatores endógenos e exógenos. Neste trabalho, realizamos a análise dos padrões de expressão dos genes de reparo de DNA encontrados na cana-de-açúcar, pela determinação da distribuição de ESTs nas diferentes bibliotecas de cDNA no projeto de transcriptoma SUCEST. Três vias de reparo - fotorreativação, reparo por excisão de bases e reparo por excisão de nucleotídeos - foram estudadas através do uso de proteínas de reparo como sondas para identificação de ESTs homólogos em cana-de-açúcar, com base na procura computacional de similaridade. Os resultados indicam que os genes de reparo de DNA possuem uma expressão diferencial nos tecidos, dependendo da via de reparo analisada. Esses dados in silico fornecem importantes indícios da expressão diferencial, a qual deve ser confirmada por análises bioquímicas diretas.

  12. Qu’est-ce que le naturalisme ?

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    Olivier Morin

    2012-01-01

    Full Text Available Cet article répond aux critiques récemment adressées aux tenants du « naturalisme social » dans les pages de la revue SociologieS. Il le fait en défendant un naturalisme minimal, bâti sur trois idées : l’interdisciplinarité est permise ; la pensée est un processus causal qui a lieu dans un monde matériel ; l’humanité est une espèce animale. Ces idées pourront sembler triviales ; le débat auquel nous assistons montre qu’il n’en est rien. Je passerai en revue les arguments les plus fréquemment opposés à ce naturalisme. Le dialogue entre sciences cognitives et sciences sociales ne prélude pas à une O.P.A. de la neurobiologie sur les autres disciplines. Aucun chercheur sérieux ne voit la cognition humaine comme un assemblage de modules à l’architecture entièrement fixée dès la naissance et incapables d’interagir avec leur environnement. Le fait que les processus mentaux n’aient pas lieu uniquement dans nos têtes est aujourd’hui un lieu commun des sciences cognitives. Enfin, l’étude de l’évolution de l’humanité, vue comme une espèce biologique, est porteuse de conclusions qui pour être très générales, n’en sont pas moins pertinentes pour les sciences sociales.What is Naturalism?This article responds to recent critics addressed to the advocates of « social naturalism » in the journal SociologieS. It does so by defending a minimal naturalism structured around three ideas: interdisciplinarity is allowed; thought is a causal process that occurs in a material world; humanity is a species. These ideas may seem trivial; the debate we’re assisting to shows us otherwise. I will go through the arguments that most frequently oppose naturalism. The dialogue between cognitive and social sciences is not a prelude to the takeover of neurobiology on other disciplines. No serious researcher sees human cognition as an assembly of architectural modules entirely fixed from birth and incapable of

  13. The pier and building of the European Solar Telescope (EST)

    Science.gov (United States)

    Bettonvil, F. C. M.; Codina, R.; Gómez Merchán, A.; Hammerschlag, R. H.; Hartman, J. J. M.; Hernández Suárez, E.; Jägers, A. P. L.; Murga Llano, G.; Pelser, J. W.; Sliepen, G.

    2010-07-01

    EST (European Solar Telescope) is a 4-m class solar telescope, which is currently in the conceptual design phase. EST will be located in the Canary Islands and will aim at high spectral, spatial and temporal resolution observations in the photosphere and chromosphere, using a suite of instruments that can produce efficiently two-dimensional spectropolarimetric information of the thermal, dynamic and magnetic properties of the plasma over many scale heights. The pier is defined as the construction that supports the telescope and the enclosure. It needs a certain height to minimize daytime ground turbulence. At the bottom of the pier a large instrument lab is located, 16 m in diameter and 10 m high. To the pier is attached a service building that accommodates all auxiliary services, possibly together with a separate building. Solid concrete- and open framework piers are compared, in terms of stability, thermal properties and flow characteristics and building structures in terms of construction issues. FE and CFD analysis are used to give qualitative insight in the differences between the alternatives. The preferred alternative is a cone shaped pier surrounded by an open framework.

  14. La seducción como estética

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    Claudia Barrera

    2012-09-01

    Full Text Available La seducción es tratada desde la literatura, la sociología, la psicología y la política como una estrategia de manipulación. Sin embargo, si se la estudia desde el campo filosófico y su tradición estética vemos sus poderes constructores de una sensibilidad poética por las fuerzas atractivas y repulsivas que contiene. La seducción, como fuerza e impulso, se inscribe en un movimiento en que el aparecer de la realidad, la imaginación y, desde luego, el arte —fuente de emotividad y sensaciones— permiten valorarla. La estética de la seducción se despliega desde la subjetividad y no desde los contenidos de la filosofía del arte. Su capacidad relacional y sus poderes condensados en el espíritu del hombre no pueden pertenecer al aparato instrumental de la sociedad de consumo o las estrategias de manipulación donde ha sido estigmatizada. Por esto la seducción es presentada como fuente de deseo y potencia vital.

  15. Arquitectura corporal: pasiones deportivas e identificaciones estéticas

    Directory of Open Access Journals (Sweden)

    Barreto Vargas, Carmen Marina

    2006-12-01

    Full Text Available In present-day societies, prevailing discourses on health, body and aesthetics regard the practice of sports as both a lifestyle and a way to constructing the body —a body increasingly constrained within certain standards of beauty in which the gender factor plays a clearly important role. The author analyzes this and other factors in the relationship between the body as a social construct and the sporting physical activity as a means that makes it possible.

    En nuestras sociedades actuales, los discursos predominantes sobre la salud, el cuerpo y la estética se vuelven hacia el deporte como un estilo de vida y como una vía de construcción del cuerpo; un cuerpo que debe, cada vez más, situarse dentro de unos estándares de belleza y, en cuya lectura, la clave de género tiene un papel ciertamente importante. El presente artículo analiza algunas de estas claves en la relación entre la construcción social del cuerpo y la práctica físico-deportiva en tanto que medio para hacerla posible.

  16. Upgrading Unconventional Oil Resources with the EST Process

    Energy Technology Data Exchange (ETDEWEB)

    Delbianco, Alberto; Meli, Salvatori; Panariti, Nicolleta; Rispoli, Giacomo

    2007-07-01

    We strongly believe that unconventional oils will play a much larger role in the growth of supply than is currently recognized. As a matter of fact, whereas the earth's conventional proven world oil reserves are 1.3 trillion barrels, extra-heavy plus bitumen resources amount to about 4 trillion barrels. The unconventional oils are characterized by low API gravity (<10), high viscosity and high concentration of poisons such as sulphur, nitrogen, metals, and asphaltenes. For this reason, a key role for the full exploitation of these hydrocarbon resources is played by the downstream processes that are required to upgrade and convert them into valuable products. In this scenario, Eni has developed a novel hydrocracking process (EST: Eni Slurry Technology) which is particularly well-suited for the conversion and upgrading of heavy feedstocks (conventional vacuum residues, extra-heavy oils and bitumen). EST employs nano-sized hydrogenation catalysts and an original process scheme that allow complete feedstock conversion to an upgraded synthetic crude oil (SCO) with an API gravity gain greater than 20 and avoid the production of residual by-products, such as pet-coke or heavy fuel oil. A Commercial Demonstration Unit (CDP) of 1200 bbl/d capacity is successfully operating in the Eni's Taranto refinery since November 2005. (auth)

  17. Fotomontajes feministas: dispositivos estético-políticos

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    Miguel Alfonso Bouhaben

    2017-12-01

    Full Text Available El presente artículo tiene el objetivo de estudiar los dispositivos estéticos y políticos que ponen en práctica los fotomontajes feministas de Martha Rosler y Barbara Kruger. Para ello, es necesario identificar cómo reaccionan estas obras contra el sistema impuesto por el patriarcado a través de la cultura mediática dominante. En los fotomontajes de Martha Rosler vamos a analizar los modos de cosificación de la mujer y las formas de control domopolítico que se establecen en el hogar. Además, nos adentraremos en los dispositivos de montaje y remontaje de imágenes, y de estructuración diferencial y deconstructiva, que la artista pone en práctica con fines emancipatorios. En el caso de Barbara Kruger nos centraremos en su crítica a los modos de representación de la mujer en los medios de comunicación. Asimismo, levantaremos acta de sus dispositivos de apropiación y de interrelación imagen-texto. Finalmente, haremos un balance de las implicaciones éticas y ontológicas que subyacen a estos dispositivos estético-políticos, en relación con las tesis defendidas por el feminismo.

  18. EVER-EST: a virtual research environment for Earth Sciences

    Science.gov (United States)

    Marelli, Fulvio; Albani, Mirko; Glaves, Helen

    2016-04-01

    There is an increasing requirement for researchers to work collaboratively using common resources whilst being geographically dispersed. By creating a virtual research environment (VRE) using a service oriented architecture (SOA) tailored to the needs of Earth Science (ES) communities, the EVEREST project will provide a range of both generic and domain specific data management services to support a dynamic approach to collaborative research. EVER-EST will provide the means to overcome existing barriers to sharing of Earth Science data and information allowing research teams to discover, access, share and process heterogeneous data, algorithms, results and experiences within and across their communities, including those domains beyond Earth Science. Researchers will be able to seamlessly manage both the data involved in their computationally intensive disciplines and the scientific methods applied in their observations and modelling, which lead to the specific results that need to be attributable, validated and shared both within the community and more widely e.g. in the form of scholarly communications. Central to the EVEREST approach is the concept of the Research Object (RO) , which provides a semantically rich mechanism to aggregate related resources about a scientific investigation so that they can be shared together using a single unique identifier. Although several e-laboratories are incorporating the research object concept in their infrastructure, the EVER-EST VRE will be the first infrastructure to leverage the concept of Research Objects and their application in observational rather than experimental disciplines. Development of the EVEREST VRE will leverage the results of several previous projects which have produced state-of-the-art technologies for scientific data management and curation as well those which have developed models, techniques and tools for the preservation of scientific methods and their implementation in computational forms such as

  19. Comprehensive EST analysis of the symbiotic sea anemone, Anemonia viridis

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    Deleury Emeline

    2009-07-01

    Full Text Available Abstract Background Coral reef ecosystems are renowned for their diversity and beauty. Their immense ecological success is due to a symbiotic association between cnidarian hosts and unicellular dinoflagellate algae, known as zooxanthellae. These algae are photosynthetic and the cnidarian-zooxanthellae association is based on nutritional exchanges. Maintenance of such an intimate cellular partnership involves many crosstalks between the partners. To better characterize symbiotic relationships between a cnidarian host and its dinoflagellate symbionts, we conducted a large-scale EST study on a symbiotic sea anemone, Anemonia viridis, in which the two tissue layers (epiderm and gastroderm can be easily separated. Results A single cDNA library was constructed from symbiotic tissue of sea anemones A. viridis in various environmental conditions (both normal and stressed. We generated 39,939 high quality ESTs, which were assembled into 14,504 unique sequences (UniSeqs. Sequences were analysed and sorted according to their putative origin (animal, algal or bacterial. We identified many new repeated elements in the 3'UTR of most animal genes, suggesting that these elements potentially have a biological role, especially with respect to gene expression regulation. We identified genes of animal origin that have no homolog in the non-symbiotic starlet sea anemone Nematostella vectensis genome, but in other symbiotic cnidarians, and may therefore be involved in the symbiosis relationship in A. viridis. Comparison of protein domain occurrence in A. viridis with that in N. vectensis demonstrated an increase in abundance of some molecular functions, such as protein binding or antioxidant activity, suggesting that these functions are essential for the symbiotic state and may be specific adaptations. Conclusion This large dataset of sequences provides a valuable resource for future studies on symbiotic interactions in Cnidaria. The comparison with the closest

  20. Plan de Punta del Este, 1935: modelo de ciudad balnearia

    Directory of Open Access Journals (Sweden)

    Juan Alberto Articardi

    2013-07-01

    Full Text Available There are a few relatively known examples that show how modern town planning in South America developed. One of the few examples is Carlos Gómez Gavazzo’s Punta del Este Town Plan that was developed between 1934 and 1935 on his return to Uruguay after having worked with Le Corbusier. The town plan was presented to the National Tourist Board as a resort city for the east coast. The concept that was applied to Punta del Este’s resort can be interpreted as a defence of modern town planning that adapted its spatial design to topographical, geographical, and climatic considerations, and transformed the urban structure as well as the peninsula’s profile.

  1. Relatório de estágio

    OpenAIRE

    Santos, Filipa R. Bolota Velho Dinis dos

    2011-01-01

    Descrevemos, no presente relatório, o percurso realizado ao longo de três meses de estágio que decorreu numa empresa de turismo com sede na Covilhã (Turistrela). O produto final nunca é um facto isolado, mas um somatório de vários factores que para ele contribuem. Neste contexto, embora o resultado global, em termos objectivos, tenha sido a realização do Website Polistrade, muito aconteceu, até à construção do mesmo. Foi uma tarefa que implicou investigação, planificação e reflexão diár...

  2. La radio no está amenazada por internet

    Directory of Open Access Journals (Sweden)

    Bruce Girard

    2015-01-01

    Full Text Available La radio es mucho más penetrante y asequible, el uso de los servicios de Internet puede contribuir a informar mejor a sus clientes y establecer interactividad. En vista que por ahora la accesibilidad del Internet es accesible solo a unos pocos, para la radio no es una amenaza el Internet. Enuncia los roles de la radio y señala que está considerada como un vínculo comunitario. Trata además sobre las radio-cadenas satelitales, la radio e Internet: convergencia para la democracia y el crecimiento de la brecha de los que acceden a la información.

  3. Inferindo personalidade por meio de estórias

    Directory of Open Access Journals (Sweden)

    Guilherme Gomes Cardoso

    2014-11-01

    Full Text Available Recentemente aspectos sutis do ser humano começaram a ser estudados e utilizados por computadores, um desses é a personalidade. Existem vários estudos que analisam como estruturá-la e extraí-la computacionalmente. No entanto, um dos gargalos no seu estado da arte atual é a forma não sutil para inferir personalidade do usuário. Este trabalho tem como objetivo mostrar o relato de uma experiência que demonstra os passos iniciais e os resultados parciais de uma pesquisa em andamento sobre um método alternativo e mais sutil para inferir a personalidade do usuário. Os resultados apresentam o uso de histórias como uma abordagem promissora, entretanto demanda novos testes baseados em metodologias estatisticamente mais consistentes.

  4. Mundus Totus Exilium Est: Reflections on the Critic in Exile

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    Robert T. Tally Jr.

    2011-05-01

    Full Text Available In his reflections on the project of literary criticism, Erich Auerbach cites the wisdom of a twelfth-century monk, who understood that while the ‘tender beginner’ cleaves to nationality, in the well-developed human being, ‘the whole world is a foreign country [mundus totus exilium est].’ What Auerbach means by quoting this is that a critic must work through his or her attachments to native soil, detach oneself from the local prejudices and comforts, and engage with one’s place as a foreigner or exile, who can thereby map such spaces critically without the distortions caused by undue familiarity. The exiles, émigrés, nomads, renegades, and refugees who create our literary maps also call for a criticism attuned to the spatial peculiarities of the conditions of exile. In an era of globalization, in which the project of literature is fundamentally transnational, the critic who can view the entire world as a foreign land is best suited to making sense of these postnational ensembles.

  5. Orden de no reanimar, consideraciones sobre este problema

    Directory of Open Access Journals (Sweden)

    Dr. G. Luis Cereceda

    2011-05-01

    Se analizan los principales problemas, comenzando por las dificultades según los diferentes escenarios (servicio de urgencia, pabellón de maternidad, sala de hospitalizados, unidad de intensivo, pabellones quirúrgicos; se analiza la incertidumbre sobre el pronóstico de cada pacientes y sobre quién(es deben tomar las decisiones sobre el fin de la vida. Idealmente debe ser el paciente autónomo quien tome la decisión, pero en otros casos se requieren alternativas, una de ellas es la Orden Unilateral. Otros problemas son la Validez de las órdenes de no-RCP en los diferentes tiempos evolutivos, el problema de la Futilidad y la Validez de las “Directivas Anticipadas”, en este grupo resalta el enfoque POLST (Physician’s Order for Life Sustaining Treatment como más comprehensivo; como complemento ha surgido también el concepto AND (Allow Natural Death, que podría estar destinado a remplazar la orden de no-RCP (DNR.

  6. Qu'est que Lila dit? Construyendo Europa

    Directory of Open Access Journals (Sweden)

    Susana Torrado Morales

    2008-01-01

    Full Text Available En este momento, Europa se encuentra inmersa en el proceso de construcción de lo que algunos llaman “alma europea”, otros “sueño europeo”, y que podemos identificar, de forma más directa, con una “identidad europea”. Una “identidad europea” definida como “…aquellos valores que los europeos comparten y que otras partes del mundo admiran”. Pero, ¿realmente existen esos valores comunes?, ¿se muestran en el cine europeo?, ¿se aprecian en películas como Lila dit ça, Monsieur Ibrahim et les fleurs du Coran o Solino? In this moment, Europe is involved in the process of making up a “European soul”, also called “European identity”. The definition of European identity is: “... those Values than European People share and other countries admire”. But, really this values exist? And, if it was the case, we can find it in the European Cinema? In films as Lila dit ça, Monsieur Ibrahim et les fleurs du Coran or Solino?

  7. Putative resistance genes in the CitEST database

    Directory of Open Access Journals (Sweden)

    Simone Guidetti-Gonzalez

    2007-01-01

    Full Text Available Disease resistance in plants is usually associated with the activation of a wide variety of defense responses to prevent pathogen replication and/or movement. The ability of the host plant to recognize the pathogen and to activate defense responses is regulated by direct or indirect interaction between the products of plant resistance (R and pathogen avirulence (Avr genes. Attempted infection of plants by avirulent pathogens elicits a battery of defenses often followed by the collapse of the challenged host cells. Localized host cell death may help to prevent the pathogen from spreading to uninfected tissues, known as hypersensitive response (HR. When either the plant or the pathogen lacks its cognate gene, activation of the plant’s defense responses fails to occur or is delayed and does not prevent pathogen colonization. In the CitEST database, we identified 1,300 reads related to R genes in Citrus which have been reported in other plant species. These reads were translated in silico, and alignments of their amino acid sequences revealed the presence of characteristic domains and motifs that are specific to R gene classes. The description of the reads identified suggests that they function as resistance genes in citrus.

  8. Estética como fisiologia aplicada em Nietzsche

    Directory of Open Access Journals (Sweden)

    Renato Bittencourt

    2010-07-01

    Full Text Available A proposta deste artigo consiste em pensarmos a relação intrínseca que Nietzsche estabelece entre a estética e a compreensão orgânica da vida humana, associada diretamente a uma perspectiva fisiológica. Cabe ressaltar que a noção de fisiologia em Nietzsche não se fundamenta em parâmetros puramente biológicos ou materiais, mas envolve todos os atos criativos do indivíduo. Por conseguinte, para compreendermos de forma precisa a realização da criatividade artística é de suma importância que se faça valer a importância da fisiologia do artista no processo de elaboração da obra de arte. Onde os instintos vitais se encontram em declínio existencial, haverá uma obra de arte decadente; onde os instintos vitais manifestam exuberância, capacidade de assimilação e saúde, haverá uma glorificação da existência através da arte.

  9. EST and microarray analysis of horn development in Onthophagus beetles

    Directory of Open Access Journals (Sweden)

    Tang Zuojian

    2009-10-01

    Full Text Available Abstract Background The origin of novel traits and their subsequent diversification represent central themes in evo-devo and evolutionary ecology. Here we explore the genetic and genomic basis of a class of traits that is both novel and highly diverse, in a group of organisms that is ecologically complex and experimentally tractable: horned beetles. Results We developed two high quality, normalized cDNA libraries for larval and pupal Onthophagus taurus and sequenced 3,488 ESTs that assembled into 451 contigs and 2,330 singletons. We present the annotation and a comparative analysis of the conservation of the sequences. Microarrays developed from the combined libraries were then used to contrast the transcriptome of developing primordia of head horns, prothoracic horns, and legs. Our experiments identify a first comprehensive list of candidate genes for the evolution and diversification of beetle horns. We find that developing horns and legs show many similarities as well as important differences in their transcription profiles, suggesting that the origin of horns was mediated partly, but not entirely, by the recruitment of genes involved in the formation of more traditional appendages such as legs. Furthermore, we find that horns developing from the head and prothorax differ in their transcription profiles to a degree that suggests that head and prothoracic horns are not serial homologs, but instead may have evolved independently from each other. Conclusion We have laid the foundation for a systematic analysis of the genetic basis of horned beetle development and diversification with the potential to contribute significantly to several major frontiers in evolutionary developmental biology.

  10. Duchamp e a anestesia estética na publicidade

    Directory of Open Access Journals (Sweden)

    João Anzanello Carrascoza

    2008-09-01

    Full Text Available A criação publicitária resulta de uma prática de bricolagem levada a termo pelo redator e pelo diretor de arte – profissionais que atuam em dupla nas agências de propaganda e são os responsáveis pela elaboração das mensagens. Nesse processo, lançam mão de todo tipo de material cultural para criar as peças solicitadas. Ambos se valem sistematicamente, como matéria-prima, de citações de imagens e enunciados fundadores, interferindo em seu significado, o que nos faz pensar nos ready-mades de Duchamp. Este artigo traz uma reflexão sobre o ready-made como método de criação na propaganda e suas implicações no mundo dos signos. Palavras-chave: Publicidade; processo criativo; bricolagem, dialogismo; ready-made. ABSTRACT The creation of advertising is the result of a bricolage process between a writer and an art director leading to an end result. These professional people work as partnership teams in advertising agencies and are responsible for the development of advertising messages. In this process, they use all forms of cultural material to stimulate them in creating the requested pieces. Both individuals systematically draw from the raw material of existing images and phrases to create meaning in the advertising in a way that makes us think of Duchamp’s ready-mades. This article is a reflection on the ready-made as a method of advertisement creation and the implications of it in the world of signs. Keywords: Advertisement; creative process; bricolage, dialogism; ready-made.

  11. Comme c'est curieux, comme c'est bizarre et quelle coïncidence ! concours de nouvelles

    CERN Document Server

    2015-01-01

    Elles sont partout : dans la nature, nichées au coeur des tournesols, des cristaux de quartz et des coquilles d'escargot sous la forme du nombre d'or ; dans nos vies ponctuées de rencontres, d'événements qui se font écho. Coïncidences : simples fruits du hasard, vraiment, ou signes d'une causalité cachée ? Un pigeon aux intestins facétieux rencontre un Président de la République malchanceux... Un obus rencontre une chambre à coucher – mais ses occupants ont rendez-vous avec un gâteau… Un dictateur qui abuse son peuple à coups de coïncidences truquées sera démasqué grâce à la rencontre inopinée d'un officier et d'un poisson… Jonglant avec les probabilités, l'Histoire et l'actualité, les lauréats de cette 6e édition ont montré, cette fois encore, que la rigueur scientifique est la meilleure amie de la fantaisie et de l'imagination. Quant à la table ronde qui ouvre ce recueil, elle croise étymologie, chaînes de causalités et lignes d'univers pour arriver à ce constat, partag�...

  12. Le bonheur n’est pas l’essentiel ?

    Directory of Open Access Journals (Sweden)

    Salome Berthon

    2007-01-01

    Full Text Available Si nos recherches ethnologiques portent en creux la marque du bonheur et du plaisir, à notre connaissance, ces notions ont rarement sinon jamais été exploitées systématiquement. Des émotions positives que l’on nous narre, en des termes souvent valorisants, aux formes du plaisir que les corps, expressifs ou silencieux, traduisent, c’est au croisement de nos terrains respectifs, pointant différents indicateurs, que nous avons tenté de définir ces notions. Visant à dépasser l’anecdotique et le liminaire au moyen d’une méthodologie « prospective », cette écriture commune fait place libre à une réflexion sur le bonheur et les plaisirs en anthropologie. Distinguant l’immédiateté du plaisir, de l’aspect différé, construit, négocié du bonheur, nous tâchons de voir en quoi, selon les situations et les relations, ils relèvent tantôt du « choisi », tantôt du « subi ». Nous portons ensuite un regard réflexif sur la position du chercheur, dont la subjectivité et les affects n’ont de cesse de mettre son ethnographie et son objectivité à l’épreuve. Si son autorité le conduit à dénier cette part émotionnelle, il devra pourtant s’engager, donner de sa personne pour gagner la confiance de ses interlocuteurs, ouvrir au discours et dévoiler quelques traces, faire surgir le non-dit des émotions, observer tant l’intime que le collectif et, in fine, comprendre ce qui du bonheur fait sens. Amorçant notre conclusion, nous nous interrogeons sur les causes et les effets de ces émotions positives, croisant pratiques et représentations, paroles et silences, automatisme et évidence, naturalité et construction, stabilité et mouvement, etc.

  13. All 5' EST - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ...n of data contents 5' EST sequences Data file File name: CSV: kome_est_5end_all.zip File URL: ftp://ftp.biosciencedbc.jp/archiv...fasta.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_est_5end_...se Description Download License Update History of This Database Site Policy | Contact Us All 5' EST - KOME | LSDB Archive ...

  14. All 3' EST - KOME | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ...n of data contents 3' EST sequences Data file File name: CSV: kome_est_3end_all.zip File URL: ftp://ftp.biosciencedbc.jp/archiv...fasta.zip File URL: ftp://ftp.biosciencedbc.jp/archive/kome/LATEST/kome_est_3end_...se Description Download License Update History of This Database Site Policy | Contact Us All 3' EST - KOME | LSDB Archive ...

  15. Alicyclobacillus acidocaldarius Thermophilic Esterase EST2's Activity in Milk and Cheese Models

    NARCIS (Netherlands)

    Mandrich, L.; Manco, M.; Rossie, M.; Floris, E.; Jansen-van den Bosch, T.; Smit, G.; Wouters, J.A.

    2006-01-01

    The aim of this work was to investigate the behavior of thermophilic esterase EST2 from Alicyclobacillus acidocaldarius in milk and cheese models. The pure enzyme was used to compare the EST2 hydrolytic activity to the activity of endogenous esterase EstA from Lactococcus lactis. The results

  16. Eine neue Methode zur Injektion und Markierung in der Chirurgie

    OpenAIRE

    Özmen, Dogan Beyhan

    2002-01-01

    Die flexible Endoskopie erlaubt ambulante Untersuchungen. Durch die komplikationslose Anwendbarkeit der Endoskope und vereinfachten Untersuchungstechniken sind die Belastungen für den Patienten weniger geworden. Die Injektionen im Gastro-Intestinal-Trakt sind immer noch schwierig durchzuführen. Sie benöt igen ein gut ausgebildetes Team mit erfahrenem Endoskopiker. Das zeigt sich insbesondere beim Markieren von Abtragungsstellen nach Polypektomien im Colon. Das ist wichtig bei R...

  17. Les contusions abdominales dans les services de chirurgie au chu ...

    African Journals Online (AJOL)

    The spleen and the liver were the most injured organs (68% and 21% of cases). All patients were operated. Total splenectomy was ... The emergency context and shortage of facilities have complicated the diagnostic procedure of abdominal contusions in our region. In consequence, clinical examination and diagnostic ...

  18. Kosteneffectiviteit van bariatrische chirurgie : Een review van economische evaluaties

    NARCIS (Netherlands)

    Suijkerbuijk, Anita; Van Gils, Paul; De Wit, Ardine; Feenstra, Talitha

    2013-01-01

    Synopsis Bariatric surgery appears to be a cost-effective intervention for moderately to severely obese people compared to single lifestyle interventions. This appears from a review of the literature on cost-effectiveness of bariatric surgery. Almost all studies included lifestyle advice before and

  19. La chirurgie conservatrice dans le cancer du rein

    African Journals Online (AJOL)

    A. Qarro

    Renal cancer;. Conservative surgery;. Nephron-sparing;. Clamping;. Cold ischemia. Conservative surgery in kidney cancer. Abstract. Kidney cancer accounts for 3% of cancers. It ranks third in urological cancers after prostate and bladder cancers. Since the radical nephrectomy described by Robson in 1963, there has been ...

  20. Malformations du tube neural en chirurgie pediatrique du chu de ...

    African Journals Online (AJOL)

    encéphalocèle et 6 cas de sipina bifida. La cure chirurgicale a été le principal mode de prise en charge des patients. Seuls 91,38% des cas ont été opérés. Pour toutes les malformations du tube neural, nous avons enregistré 7 décès dont 2 décès ...

  1. Die plastisch-rekonstruktive Chirurgie aus Sicht der Kinderchirurgie

    Directory of Open Access Journals (Sweden)

    Finke, Rainer

    2013-02-01

    Full Text Available Plastic reconstructive surgery from a pediatric surgical point of view means more than to apply adult surgical techniques and methods to pediatric conditions. A child’s multidimensional characteristics and particularity, development potential, wishes and expectations must be respected during planning and conduction of surgical procedures. In this article the relevance of the four pillars of plastic surgery for the different phases of childhood are portrayed. Especially the legal and ethnic problems of treatment modalities of disorders of sex differentiation are discussed, particularly taking into consideration the heterogeneity of this entity. We see no indication for gender assigning operations during childhood since an informed consent cannot be obtained due to the age of the patient. A cosmetic and only gender emphasizing operation for a child with congenital adrenal hyperplasia may only be performed in concordance with the guidelines of the German Ethics Council and after extensive and independent consultation.

  2. Chirurgie endoscopique de l'appareil urinaire au CHU Sylvanus ...

    African Journals Online (AJOL)

    Le but de cette étude étant de souligner les insuffisances et les points forts de cette technique au plan national puis d'accroître cette spécificité chirurgicale très bénéfique, au plan économie et de durée de séjour réduit pour la prise en charge des patients, malgré le système d'entretien énorme sans cesse croissant au plan ...

  3. Antibioprophylaxie en chirurgie propre et propre-contaminee ...

    African Journals Online (AJOL)

    Methodology and results: We carried out a prospective study to compare two groups: one (A) with 74 patients subjected to antibiotic prophylaxis; the other (B) with 87 patients subjected to seven days administration of probabilistic antibiotherapy. The rates of infections were 2.7% and 3.4% respectively in the group A and B ...

  4. La morbidite infectieuse post-operatoire en chirurgie ...

    African Journals Online (AJOL)

    In sub-Saharan Africa, there is an increased incidence of postoperative infection in gynaecological and obstetrical surgical interventions. In the present study, we sought to describe the morbidity of post-operative infections at the University Teaching Hospital. This should prompt us to evaluate the efficiency of the fight ...

  5. Relatório de estágio II de Paula Cristina Paiva da Rocha

    OpenAIRE

    Rocha, Paula Cristina Paiva da

    2017-01-01

    Este relatório de estágio encontra-se inserido na unidade curricular Estágio II, no âmbito do curso, Mestrado Integrado em Ciências Farmacêuticas. Este estágio apresenta a duração total de cinco meses, sendo que 3 meses decorrem em Farmácia Hospitalar, e os restantes 2 meses decorrem em Farmácia Comunitária. Este relatório de estágio consiste na descrição de todas as atividades desenvolvidas no seu decorrer, tanto nos Serviços Farmacêuticos do Hospital General Universitário de Castellón, ...

  6. Construction of full-length cDNA library and development of EST-derived simple sequence repeat (EST-SSR) markers in Senecio scandens.

    Science.gov (United States)

    Qian, Gang; Ping, Junjiao; Lu, Jian; Zhang, Zhen; Wang, Lei; Xu, Delin

    2014-12-01

    Senecio scandens Buch.-Ham. ex D. Don (Compositae) is a crucial source of Chinese traditional medicine with antibacterial properties. We constructed a cDNA library and obtained expressed sequence tags (ESTs) to show the distribution of gene ontology annotations for mRNAs, using an individual plant with superior antibacterial characteristics. Analysis of comparative genomics indicates that the putative uncharacterized proteins (21.07%) might be derived from "molecular function unknown" clones or rare transcripts. Furthermore, the Compositae had high cross-species transferability of EST-derived simple sequence repeats (EST-SSR), based on valid amplifications of 206 primer pairs developed from the newly assembled expressed sequence tag sequences in Artemisia annua L. Among those EST-SSR markers, 52 primers showed polymorphic amplifications between individuals with contrasting diverse antibacterial traits. Our sequence data and molecular markers will be cost-effective tools for further studies such as genome annotation, molecular breeding, and novel transcript profiles within Compositae species.

  7. Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug Phenacoccus solenopsis.

    Science.gov (United States)

    Li, Hui; Lang, Kun-Ling; Fu, Hai-Bin; Shen, Chang-Peng; Wan, Fang-Hao; Chu, Dong

    2015-12-01

    The cotton mealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution. © 2014 Institute of Zoology, Chinese Academy of Sciences.

  8. EST2uni: an open, parallel tool for automated EST analysis and database creation, with a data mining web interface and microarray expression data integration.

    Science.gov (United States)

    Forment, Javier; Gilabert, Francisco; Robles, Antonio; Conejero, Vicente; Nuez, Fernando; Blanca, Jose M

    2008-01-07

    Expressed sequence tag (EST) collections are composed of a high number of single-pass, redundant, partial sequences, which need to be processed, clustered, and annotated to remove low-quality and vector regions, eliminate redundancy and sequencing errors, and provide biologically relevant information. In order to provide a suitable way of performing the different steps in the analysis of the ESTs, flexible computation pipelines adapted to the local needs of specific EST projects have to be developed. Furthermore, EST collections must be stored in highly structured relational databases available to researchers through user-friendly interfaces which allow efficient and complex data mining, thus offering maximum capabilities for their full exploitation. We have created EST2uni, an integrated, highly-configurable EST analysis pipeline and data mining software package that automates the pre-processing, clustering, annotation, database creation, and data mining of EST collections. The pipeline uses standard EST analysis tools and the software has a modular design to facilitate the addition of new analytical methods and their configuration. Currently implemented analyses include functional and structural annotation, SNP and microsatellite discovery, integration of previously known genetic marker data and gene expression results, and assistance in cDNA microarray design. It can be run in parallel in a PC cluster in order to reduce the time necessary for the analysis. It also creates a web site linked to the database, showing collection statistics, with complex query capabilities and tools for data mining and retrieval. The software package presented here provides an efficient and complete bioinformatics tool for the management of EST collections which is very easy to adapt to the local needs of different EST projects. The code is freely available under the GPL license and can be obtained at http://bioinf.comav.upv.es/est2uni. This site also provides detailed instructions

  9. EST2uni: an open, parallel tool for automated EST analysis and database creation, with a data mining web interface and microarray expression data integration

    Directory of Open Access Journals (Sweden)

    Nuez Fernando

    2008-01-01

    Full Text Available Abstract Background Expressed sequence tag (EST collections are composed of a high number of single-pass, redundant, partial sequences, which need to be processed, clustered, and annotated to remove low-quality and vector regions, eliminate redundancy and sequencing errors, and provide biologically relevant information. In order to provide a suitable way of performing the different steps in the analysis of the ESTs, flexible computation pipelines adapted to the local needs of specific EST projects have to be developed. Furthermore, EST collections must be stored in highly structured relational databases available to researchers through user-friendly interfaces which allow efficient and complex data mining, thus offering maximum capabilities for their full exploitation. Results We have created EST2uni, an integrated, highly-configurable EST analysis pipeline and data mining software package that automates the pre-processing, clustering, annotation, database creation, and data mining of EST collections. The pipeline uses standard EST analysis tools and the software has a modular design to facilitate the addition of new analytical methods and their configuration. Currently implemented analyses include functional and structural annotation, SNP and microsatellite discovery, integration of previously known genetic marker data and gene expression results, and assistance in cDNA microarray design. It can be run in parallel in a PC cluster in order to reduce the time necessary for the analysis. It also creates a web site linked to the database, showing collection statistics, with complex query capabilities and tools for data mining and retrieval. Conclusion The software package presented here provides an efficient and complete bioinformatics tool for the management of EST collections which is very easy to adapt to the local needs of different EST projects. The code is freely available under the GPL license and can be obtained at http://bioinf.comav.upv.es/est

  10. A experiência estética sob um olhar fenomenológico

    Directory of Open Access Journals (Sweden)

    Alice Casanova Reis

    2011-01-01

    Full Text Available Este artículo describe la experiencia estética como un fenómeno centrado en la percepción sensorial. El trabajo se desarrolla a partir de una investigación de la psicología social basado en la fenomenología, sobre todo en los planteamientos de Merleau-Ponty y Dufrenne. Concibe la experiencia estética como un modo de relación mediada por la percepción sensorial de un objeto estético. Se analizan diversos aspectos de la experiencia estética: la dimensión relacional y sensible; ¿cómo se constituye el sujeto y el objeto estético; la dimensión corporal de la experiencia estética; la intencionalidad en la estética; la experiencia estética de la naturaleza. Llegamos a la conclusión de que hay un significado ontológico de la experiencia estética, ya que proporciona una apertura a la diferencia por el contacto con la alteridad, y una nueva mirada a la realidad. Se destaca al final la relevancia de esta experiencia a la Psicología Social.

  11. Development and production of an oligonucleotide MuscleChip: use for validation of ambiguous ESTs

    Directory of Open Access Journals (Sweden)

    Lanfranchi Gerolamo

    2002-10-01

    Full Text Available Abstract Background We describe the development, validation, and use of a highly redundant 120,000 oligonucleotide microarray (MuscleChip containing 4,601 probe sets representing 1,150 known genes expressed in muscle and 2,075 EST clusters from a non-normalized subtracted muscle EST sequencing project (28,074 EST sequences. This set included 369 novel EST clusters showing no match to previously characterized proteins in any database. Each probe set was designed to contain 20–32 25 mer oligonucleotides (10–16 paired perfect match and mismatch probe pairs per gene, with each probe evaluated for hybridization kinetics (Tm and similarity to other sequences. The 120,000 oligonucleotides were synthesized by photolithography and light-activated chemistry on each microarray. Results Hybridization of human muscle cRNAs to this MuscleChip (33 samples showed a correlation of 0.6 between the number of ESTs sequenced in each cluster and hybridization intensity. Out of 369 novel EST clusters not showing any similarity to previously characterized proteins, we focused on 250 EST clusters that were represented by robust probe sets on the MuscleChip fulfilling all stringent rules. 102 (41% were found to be consistently "present" by analysis of hybridization to human muscle RNA, of which 40 ESTs (39% could be genome anchored to potential transcription units in the human genome sequence. 19 ESTs of the 40 ESTs were furthermore computer-predicted as exons by one or more than three gene identification algorithms. Conclusion Our analysis found 40 transcriptionally validated, genome-anchored novel EST clusters to be expressed in human muscle. As most of these ESTs were low copy clusters (duplex and triplex in the original 28,000 EST project, the identification of these as significantly expressed is a robust validation of the transcript units that permits subsequent focus on the novel proteins encoded by these genes.

  12. Rapid in silico cloning of genes using expressed sequence tags (ESTs).

    Science.gov (United States)

    Gill, R W; Sanseau, P

    2000-01-01

    Expressed sequence tags (ESTs) are short single-pass DNA sequences obtained from either end of cDNA clones. These ESTs are derived from a vast number of cDNA libraries obtained from different species. Human ESTs are the bulk of the data and have been widely used to identify new members of gene families, as markers on the human chromosomes, to discover polymorphism sites and to compare expression patterns in different tissues or pathologies states. Information strategies have been devised to query EST databases. Since most of the analysis is performed with a computer, the term "in silico" strategy has been coined. In this chapter we will review the current status of EST databases, the pros and cons of EST-type data and describe possible strategies to retrieve meaningful information.

  13. Lecture actentielle dans Trop c'est trop de Protais Asseng | IKANGA ...

    African Journals Online (AJOL)

    La naissance de cet enfant lui conférait le titre de "Papa national" Bakony symbolise la recherche de bonheur sur le dos d'un humain. C'est de l'exploitation de la femme par l'homme. Mais la multipare Bissabey, son épouse, n'est pas de son avis. Cette instrumentalisation est alors la pomme de discorde entre le couple et ...

  14. Towards the ictalurid catfish transcriptome: generation and analysis of 31,215 catfish ESTs

    Directory of Open Access Journals (Sweden)

    Dunham Rex

    2007-06-01

    Full Text Available Abstract Background EST sequencing is one of the most efficient means for gene discovery and molecular marker development, and can be additionally utilized in both comparative genome analysis and evaluation of gene duplications. While much progress has been made in catfish genomics, large-scale EST resources have been lacking. The objectives of this project were to construct primary cDNA libraries, to conduct initial EST sequencing to generate catfish EST resources, and to obtain baseline information about highly expressed genes in various catfish organs to provide a guide for the production of normalized and subtracted cDNA libraries for large-scale transcriptome analysis in catfish. Results A total of 17 cDNA libraries were constructed including 12 from channel catfish (Ictalurus punctatus and 5 from blue catfish (I. furcatus. A total of 31,215 ESTs, with average length of 778 bp, were generated including 20,451 from the channel catfish and 10,764 from blue catfish. Cluster analysis indicated that 73% of channel catfish and 67% of blue catfish ESTs were unique within the project. Over 53% and 50% of the channel catfish and blue catfish ESTs, respectively, had significant similarities to known genes. All ESTs have been deposited in GenBank. Evaluation of the catfish EST resources demonstrated their potential for molecular marker development, comparative genome analysis, and evaluation of ancient and recent gene duplications. Subtraction of abundantly expressed genes in a variety of catfish tissues, identified here, will allow the production of low-redundancy libraries for in-depth sequencing. Conclusion The sequencing of 31,215 ESTs from channel catfish and blue catfish has significantly increased the EST resources in catfish. The EST resources should provide the potential for microarray development, polymorphic marker identification, mapping, and comparative genome analysis.

  15. La estética figural en la última escritura y trabajos visuales de Enrique Lihn

    OpenAIRE

    Juan Zapata Gacitúa; Mariela Fuentes Leal

    2015-01-01

    En este artículo analizo la última producción creativa de Enrique Lihn; por una parte, la que está vinculada con su poesía y, por otra parte, la relacionada con sus trabajos visuales. Ambas variantes de su trabajo están en una estrecha relación hacia el final de su vida, lo cual es posible demostrar desde la estética figural de Jean-Francois Lyotard y sobre la base de la crítica que ha estudiado la etapa final de la vida y de la obra del creador chileno.

  16. La estética del entorno y el cognitivismo científico

    OpenAIRE

    Rosa Martínez, María del Mar

    2014-01-01

    Abandonado el estudio de la naturaleza durante casi dos siglos, la estética del entorno entronca hoy con la estética de la naturaleza de Kant y debate sus presupuestos en dos posiciones fundamentales: cognitivismo y no-cognitivismo. En particular, el llamado cognitivismo científico reclama para el juicio estético la mediación imprescindible de conceptos científicos. En el artículo se discute la teoría de Allen Carlson, su principal representante, y se compara con la estética no cognitivista d...

  17. Hermon est

    OpenAIRE

    Aliquot, Julien

    2012-01-01

    105. Deir el-Aachaiyer Architecture : Krencker & Zschietzschmann 1938, p. 256-264, 292-293, pl. 110-115 et 118, 27 (état des lieux en 1905, compléments en 1933) ; Dentzer-Feydy 1999, p. 531-532 et 551 (notes et dessins de W.J. Bankes) ; Chéhab 1949-1950, p. 111 (tête tourelée de Tyché). Photos : Taylor 1971, p. 86-89 ; Blas de Roblès, Pieri & Yon 2004, p. 179-180 ; Nordiguian 2005, p. 104-105. Site revu. Épigraphie : IGLS 11, 4 (dédicace). Le village moderne de Deir el-Aachaiyer (1250 m d’alt...

  18. Analysis of inheritance mode in chrysanthemum using EST-derived SSR markers

    NARCIS (Netherlands)

    Park, Sang Kun; Arens, Paul; Esselink, Danny; Lim, Jin Hee; Shin, Hak Ki

    2015-01-01

    To study the inheritance mode of hexaploid chrysanthemum (random or preferential chromosome pairing), a segregation analysis was carried out using SSR markers derived from chrysanthemum ESTs in the public domain. A total of 248 EST-SSR primer pairs were screened in chrysanthemum cultivars

  19. Bajo un azul dilatado : Ensayo para una estética musical sudamericana

    OpenAIRE

    Menacho, Luis

    2015-01-01

    Tesis doctoral acerca de lineamientos desde donde pensar una estética musical de tradición escrita en la música sudamericana compuesta después de 1960. El abordaje de análisis de obras y compositores, sus vinculaciones contextuales y propuestas estéticas a la luz de un pensar sudamericano. Facultad de Bellas Artes

  20. Development of genomic SSR and potential EST-SSR markers in ...

    African Journals Online (AJOL)

    In addition, forty four EST-SSRs which can be amplified with expected sizes were identified from a B. chinense root cDNA library. The genomic SSR markers and potential EST-SSR markers developed in the present study should be useful for genetic diversity and molecular marker assistant selection breeding research in ...

  1. In silico comparative analysis of EST-SSRs in three cotton genomes

    African Journals Online (AJOL)

    reading 6

    2012-08-28

    Aug 28, 2012 ... In this study, expressed sequence tags- simple sequence repeat (EST-SSRs) were surveyed in three cotton genomes (Gossypium arboreum, Ga; Gossypium raimondii, Gr and Gossypium hirsutum, Gh). The frequency of EST-SSRs was highest in Gr, and motif type for hexanucleotide was obviously.

  2. The Reality of Fiction and the Ethics of Storytelling in Eleanor Estes's "The Witch Family"

    Science.gov (United States)

    Heinecken, Dawn

    2010-01-01

    This essay examines Eleanor Estes's critically neglected 1960 novel "The Witch Family", arguing that the novel anticipates some of the major preoccupations of later children's literature in its early concern with issues of textuality. While Estes is largely known as a writer of simple family stories, "The Witch Family" is an innovative work of…

  3. A second essential function of the Est1-binding arm of yeast telomerase RNA.

    Science.gov (United States)

    Lebo, Kevin J; Niederer, Rachel O; Zappulla, David C

    2015-05-01

    The enzymatic ribonucleoprotein telomerase maintains telomeres in many eukaryotes, including humans, and plays a central role in aging and cancer. Saccharomyces cerevisiae telomerase RNA, TLC1, is a flexible scaffold that tethers telomerase holoenzyme protein subunits to the complex. Here we test the hypothesis that a lengthy conserved region of the Est1-binding TLC1 arm contributes more than simply Est1-binding function. We separated Est1 binding from potential other functions by tethering TLC1 to Est1 via a heterologous RNA-protein binding module. We find that Est1-tethering rescues in vivo function of telomerase RNA alleles missing nucleotides specifically required for Est1 binding, but not those missing the entire conserved region. Notably, however, telomerase function is restored for this condition by expressing the arm of TLC1 in trans. Mutational analysis shows that the Second Essential Est1-arm Domain (SEED) maps to an internal loop of the arm, which SHAPE chemical mapping and 3D modeling suggest could be regulated by conformational change. Finally, we find that the SEED has an essential, Est1-independent role in telomerase function after telomerase recruitment to the telomere. The SEED may be required for establishing telomere extendibility or promoting telomerase RNP holoenzyme activity. © 2015 Lebo et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

  4. Fukushima - radiological impact on the Central Europe simulated by the system ESTE

    International Nuclear Information System (INIS)

    Carny, P.; Suchon, D.; Smejkalova, E.; Chyly, M.; Krpelanova, M.; Liptak, L.

    2011-01-01

    Radiological impact of the events at nuclear installations Fukushima Dai-ichi on the Central Europe were modelled by system ESTE. Events in Fukushima and their radiological impacts are modelled mainly due to verification of our approaches, algorithms and models of ESTE. (authors)

  5. Bin mapping of genomic and EST-derived SSRs in melon (Cucumis melo L.).

    Science.gov (United States)

    Fernandez-Silva, I; Eduardo, I; Blanca, J; Esteras, C; Picó, B; Nuez, F; Arús, P; Garcia-Mas, J; Monforte, Antonio José

    2008-12-01

    We report the development of 158 primer pairs flanking SSR motifs in genomic (gSSR) and EST (EST-SSR) melon sequences, all yielding polymorphic bands in melon germplasm, except one that was polymorphic only in Cucurbita species. A similar polymorphism level was found among EST-SSRs and gSSRs, between dimeric and trimeric EST-SSRs, and between EST-SSRs placed in the open reading frame or any of the 5'- or 3'-untranslated regions. Correlation between SSR length and polymorphism was only found for dinucleotide EST-SSRs located within the untranslated regions, but not for trinucleotide EST-SSRs. Transferability of EST-SSRs to Cucurbita species was assayed and 12.7% of the primer pairs amplified at least in one species, although only 5.4% were polymorphic. A set of 14 double haploid lines from the cross between the cultivar "Piel de Sapo" and the accession PI161375 were selected for the bin mapping approach in melon. One hundred and twenty-one SSR markers were newly mapped. The position of 46 SSR loci was also verified by genotyping the complete population. A final bin-map was constructed including 80 RFLPs, 212 SSRs, 3 SNPs and the Nsv locus, distributed in 122 bins with an average bin length of 10.2 cM and a maximum bin length of 33 cM. Map density was 4.2 cM/marker or 5.9 cM/SSR.

  6. Ya no estás más a mi lado, corazón: estética Camp en América Latina

    OpenAIRE

    Hueso Fibla, Silvia

    2012-01-01

    Esta tesis doctoral consta de tres capítulos cuyo recorrido pretende ilustrar que la puerta de entrada a la Estética Camp pasa por una mirada diferente sobre los productos culturales de masas y que su puerta de salida en América Latina atraviesa la Estética Neobarroca; por tanto, el Camp resulta tanto física como conceptualmente central en el trabajo. Se inserta el análisis de tres novelas y una obra teatral para mostrar cuáles son los mecanismos que el Camp adopta en América Latina: Tengo mi...

  7. La música de Beethoven en el pensament estètic d'Adorno

    OpenAIRE

    Custodio de Alba, Josep

    2007-01-01

    Aquest treball té com a finalitat una reconstrucció interpretativa del pensament estètic d'Adorno sobre la música de Beethoven i una valoració de la pretensió adorniana de fer de la interpretació estètica de Beethoven el punt de partida de la seva filosofia de la música. Este trabajo tiene como finalidad una reconstrucción interpretativa del pensamiento estético de Adorno sobre la música de Beethoven y una valoración de la pretensión adorniana de hacer de la interpretación estética de Beet...

  8. A estética política das mídias locativas

    Directory of Open Access Journals (Sweden)

    Lucia Santaella

    2008-04-01

    Full Text Available Las prácticas de acceso propiciadas por los dispositivos móviles conectados al Sistema de Posicionamiento Global (GPS están haciendo surgir un nuevo espacio social de combinaciones inextricables entre lo virtual (el ciberespacio y los ambientes físicos en los que se mueve nuestro cuerpo biológico. Este trabajo busca presentar las nuevas modalidades de comunicación, conexión e interacción que están apareciendo bajo el nombre de “medios de comunicación locativos”. Éstos son procesos que están dando origen a una estética políticamente orientada, cuyas principales características se discutirán en el artículo.

  9. Effets cardiovasculaires d'un bloqueur calcique en hypoxie d'altitude

    DEFF Research Database (Denmark)

    Dugas, L; Dubray, C; Herry, J P

    1995-01-01

    High altitude pulmonary oedema can be successfully treated and prevented by calcium channel blockers. Moreover, calcium entering in the cells could explain the congestive phenomena of acute mountain sickness (AMS). These findings led us to study the action of a calcium channel blocker, isradipine...

  10. Effect of ionizing radiation on cardiovascular system; Les effets des rayonnements ionisants sur le systeme cardiovasculaire

    Energy Technology Data Exchange (ETDEWEB)

    Milliat, F.; Benderitter, M.; Gaugler, M.H. [Institut de radioprotection et de surete nucleaire, DRPH, SRBE, Laboratoire de radiopathologie et de therapies experimentales (LRTE), Fontenay-aux-Roses (France)

    2011-07-01

    Radiotherapy treatment for cancer of the chest, mediastinal area or the neck area is associated with increased risk of cardiovascular disease. With the increasing number of cancer patients and the increased treatment efficiency, the number of cancer survivors is increasing exponentially. The cancer survivors live longer and their long-term follow-up must be considered. The cardiovascular toxicity is mainly associated with the treatment of breast cancer, Hodgkin's lymphoma and head and neck cancer. Radiation-induced cardiovascular effects are insidious and chronic. Their occurrence is linked to numerous factors including the age of the patient at the beginning of the radiotherapy schedule, the number of years following radiotherapy, the doses (and volume) to the heart and the large vessels (coronary and carotid arteries), and the association with the traditional cardiovascular risk factors. Pathophysiological mechanisms remain unclear and, even if similarities with age-related atherosclerosis were established, the specificities of the radiation-induced atherosclerosis for high doses remain to be discovered. For low/moderate doses of ionising radiation, recent epidemiological studies provide evidence of increased risk of cardiovascular pathologies. A better knowledge of the mechanisms associated with the radiation-induced cardiovascular pathologies and the more precise identification of the populations at risk in the future should allow a more effective care of these patients with cardiovascular risk. (authors)

  11. Acides gras oméga-3 et risque cardiovasculaire

    Directory of Open Access Journals (Sweden)

    Dallongeville Jean

    2010-07-01

    Full Text Available Linoleic acid (LA and alpha-linolenic acid (ALA are precursors of omega-6 and omega-3 fatty acids. Eicosapentaenoic acid (EPA and docosahexaenoic acid (DHA are synthesized from ALA. These fatty acids play important role in various biological functions. The effects of omega-3 on cardiovascular risk factors – such as lipoprotein levels, blood pressure and glycemia – are very modest at standard low doses, significant only for large intakes. Randomized trials have shown that oils enriched in EPA and DHA have an undeniable impact on heart rhythm. Consumers of long-chain fatty acid have lower heart rates than controls. Conversely, patients with established arrhythmias do not appear to benefit from treatment with these fatty acids. The observation of food habits in population cohorts helped to defined the effects of rich in ALA, EPA, DHA on the risk of myocardial infarction, coronary heart disease and sudden death. For ALA the results are inconsistent and appropriate randomized clinical trials are still conclude. The effects of EPA and DHA are better established. The meta-analysis of cohort studies shows that consumption of omega-3 long- chain (EPA-DHA reduces total mortality, death from cardiac causes, sudden death and possibly stroke. The data from prevention trials with omega-3 long-chain showed a decrease in coronary events, including fatal, in secondary prevention. These results are relatively consistent to believe that EPA and DHA intake reduces the fatal complications of myocardial infarction. Clinical trials in primary prevention are still missing. However, the sum of evidence support the recommendation for consumption of fish, EPA and DHA in patients at high risk of coronary event.

  12. SolEST database: a "one-stop shop" approach to the study of Solanaceae transcriptomes.

    Science.gov (United States)

    D'Agostino, Nunzio; Traini, Alessandra; Frusciante, Luigi; Chiusano, Maria Luisa

    2009-11-30

    Since no genome sequences of solanaceous plants have yet been completed, expressed sequence tag (EST) collections represent a reliable tool for broad sampling of Solanaceae transcriptomes, an attractive route for understanding Solanaceae genome functionality and a powerful reference for the structural annotation of emerging Solanaceae genome sequences. We describe the SolEST database http://biosrv.cab.unina.it/solestdb which integrates different EST datasets from both cultivated and wild Solanaceae species and from two species of the genus Coffea. Background as well as processed data contained in the database, extensively linked to external related resources, represent an invaluable source of information for these plant families. Two novel features differentiate SolEST from other resources: i) the option of accessing and then visualizing Solanaceae EST/TC alignments along the emerging tomato and potato genome sequences; ii) the opportunity to compare different Solanaceae assemblies generated by diverse research groups in the attempt to address a common complaint in the SOL community. Different databases have been established worldwide for collecting Solanaceae ESTs and are related in concept, content and utility to the one presented herein. However, the SolEST database has several distinguishing features that make it appealing for the research community and facilitates a "one-stop shop" for the study of Solanaceae transcriptomes.

  13. Analysis of tall fescue ESTs representing different abiotic stresses, tissue types and developmental stages

    Directory of Open Access Journals (Sweden)

    Zhao Xuechun

    2008-03-01

    Full Text Available Abstract Background Tall fescue (Festuca arundinacea Schreb is a major cool season forage and turf grass species grown in the temperate regions of the world. In this paper we report the generation of a tall fescue expressed sequence tag (EST database developed from nine cDNA libraries representing tissues from different plant organs, developmental stages, and abiotic stress factors. The results of inter-library and library-specific in silico expression analyses of these ESTs are also reported. Results A total of 41,516 ESTs were generated from nine cDNA libraries of tall fescue representing tissues from different plant organs, developmental stages, and abiotic stress conditions. The Festuca Gene Index (FaGI has been established. To date, this represents the first publicly available tall fescue EST database. In silico gene expression studies using these ESTs were performed to understand stress responses in tall fescue. A large number of ESTs of known stress response gene were identified from stressed tissue libraries. These ESTs represent gene homologues of heat-shock and oxidative stress proteins, and various transcription factor protein families. Highly expressed ESTs representing genes of unknown functions were also identified in the stressed tissue libraries. Conclusion FaGI provides a useful resource for genomics studies of tall fescue and other closely related forage and turf grass species. Comparative genomic analyses between tall fescue and other grass species, including ryegrasses (Lolium sp., meadow fescue (F. pratensis and tetraploid fescue (F. arundinacea var glaucescens will benefit from this database. These ESTs are an excellent resource for the development of simple sequence repeat (SSR and single nucleotide polymorphism (SNP PCR-based molecular markers.

  14. Estándares de calidad de la información y democracia de calidad

    Directory of Open Access Journals (Sweden)

    José Villamarin Carrascal

    2013-06-01

    Full Text Available Este artículo propone seis estándares de calidad de la información, indicadores mínimos para el desarrollo de un periodismo ético, responsable y creador de ciudadanía. Se complementa con casos de la prensa nacional y mundial en los que la ausencia de esos estándares puede terminar en evidentes formas de manipulación. Propone que la recuperación de la credibilidad de un discurso público como el periodismo depende de la rigurosidad de sus prácticas informativas.

  15. ESTIMA, a tool for EST management in a multi-project environment.

    Science.gov (United States)

    Kumar, Charu G; LeDuc, Richard; Gong, George; Roinishivili, Levan; Lewin, Harris A; Liu, Lei

    2004-11-04

    Single-pass, partial sequencing of complementary DNA (cDNA) libraries generates thousands of chromatograms that are processed into high quality expressed sequence tags (ESTs), and then assembled into contigs representative of putative genes. Usually, to be of value, ESTs and contigs must be associated with meaningful annotations, and made available to end-users. A web application, Expressed Sequence Tag Information Management and Annotation (ESTIMA), has been created to meet the EST annotation and data management requirements of multiple high-throughput EST sequencing projects. It is anchored on individual ESTs and organized around different properties of ESTs including chromatograms, base-calling quality scores, structure of assembled transcripts, and multiple sources of comparison to infer functional annotation, Gene Ontology associations, and cDNA library information. ESTIMA consists of a relational database schema and a set of interactive query interfaces. These are integrated with a suite of web-based tools that allow a user to query and retrieve information. Further, query results are interconnected among the various EST properties. ESTIMA has several unique features. Users may run their own EST processing pipeline, search against preferred reference genomes, and use any clustering and assembly algorithm. The ESTIMA database schema is very flexible and accepts output from any EST processing and assembly pipeline. ESTIMA has been used for the management of EST projects of many species, including honeybee (Apis mellifera), cattle (Bos taurus), songbird (Taeniopygia guttata), corn rootworm (Diabrotica vergifera), catfish (Ictalurus punctatus, Ictalurus furcatus), and apple (Malus x domestica). The entire resource may be downloaded and used as is, or readily adapted to fit the unique needs of other cDNA sequencing projects. The scripts used to create the ESTIMA interface are freely available to academic users in an archived format from http

  16. ESTIMA, a tool for EST management in a multi-project environment

    Directory of Open Access Journals (Sweden)

    Lewin Harris A

    2004-11-01

    Full Text Available Abstract Background Single-pass, partial sequencing of complementary DNA (cDNA libraries generates thousands of chromatograms that are processed into high quality expressed sequence tags (ESTs, and then assembled into contigs representative of putative genes. Usually, to be of value, ESTs and contigs must be associated with meaningful annotations, and made available to end-users. Results A web application, Expressed Sequence Tag Information Management and Annotation (ESTIMA, has been created to meet the EST annotation and data management requirements of multiple high-throughput EST sequencing projects. It is anchored on individual ESTs and organized around different properties of ESTs including chromatograms, base-calling quality scores, structure of assembled transcripts, and multiple sources of comparison to infer functional annotation, Gene Ontology associations, and cDNA library information. ESTIMA consists of a relational database schema and a set of interactive query interfaces. These are integrated with a suite of web-based tools that allow a user to query and retrieve information. Further, query results are interconnected among the various EST properties. ESTIMA has several unique features. Users may run their own EST processing pipeline, search against preferred reference genomes, and use any clustering and assembly algorithm. The ESTIMA database schema is very flexible and accepts output from any EST processing and assembly pipeline. ESTIMA has been used for the management of EST projects of many species, including honeybee (Apis mellifera, cattle (Bos taurus, songbird (Taeniopygia guttata, corn rootworm (Diabrotica vergifera, catfish (Ictalurus punctatus, Ictalurus furcatus, and apple (Malus x domestica. The entire resource may be downloaded and used as is, or readily adapted to fit the unique needs of other cDNA sequencing projects. Conclusions The scripts used to create the ESTIMA interface are freely available to academic users in

  17. La importancia de la experiencia estética y el arte actual

    OpenAIRE

    Tejada, Jesús

    2003-01-01

    Es imprescindible que la reflexión estética aborde el problema de la crisis del arte actual y que analice las distintas respuestas que en la práctica se le están dando. La renovación del diálogo con planteamientos estéticos tradicionales puede arrojar una nueva luz sobre la actividad de la creatividad artística, al destacar los conceptos de emoción y conocimiento entre otros.

  18. Literatura chilena en digital: mapas, estéticas y conceptualizaciones

    OpenAIRE

    Carolina Gaínza C.

    2017-01-01

    En el presente artículo se busca analizar una experiencia estética vinculada a lo digital, que tiene como principales componentes la posibilidad de interactuar, participar y manipular las obras creadas en este formato. Con tal fin, se presentará un mapa de la literatura digital en Chile, para luego profundizar en el análisis de dos aspectos que permiten caracterizar una estética de lo digital: la hipertextualidad y el hackeo cultural. A partir de estos elementos y tomando en cuenta que la lit...

  19. Restauraciones estéticas en el sector anterior con resina compuesta. reporte de un caso.

    OpenAIRE

    Perdomo Pacheco, Yecenia

    2011-01-01

    Las carillas directas se están convirtiendo en una solución para casi todos los problemas estéticos de la odontología actual. Los nuevos materiales de resina compuesta aparecidos en los últimos años han mejorado de forma importante sus propiedades mecánicas y estéticas, sobre todo la estabilidad del color, la textura y el pulido. Es por eso que cuando un paciente tiene una sonrisa con pequeñas alteraciones dentales, el uso de tratamientos conservadores tales como recontorneado gingival y/o gi...

  20. Relatório de estágio II de Catarina Sofia Fernandes da Costa

    OpenAIRE

    Nunes, Catarina Sofia Moreira

    2017-01-01

    O presente relatório de estágio, elaborado no âmbito na unidade curricular estágio II do Mestrado Integrado em Ciências Farmacêuticas do Instituto Universitário de Ciências da Saúde, aborda as atividades do farmacêutico hospitalar e do farmacêutico comunitário, dividindo-se, por isso, em duas partes. O estágio hospitalar decorreu entre março e abril, no Hospital Pedro Hispano, no qual houve oportunidade de objetivar todas as atividades inerentes ao farmacêutico hospitalar, e ao circuito d...

  1. El aporte literario y estético de the Beatles

    Directory of Open Access Journals (Sweden)

    Gustavo Hernández Castro

    2013-07-01

    Full Text Available Este artículo intenta dilucidar el aporte literario y estético de la banda de rock and roll The Beatles a partir del reacomodo económico e ideológico moderno de la segunda mitad del siglo veinte. El punto de partida, para este análisis, es el pensamiento existencialista, específicamente las categorías del “absurdo”, “ser” y la “nada”, como conceptos de una espiral histórica en contradicción para nuestro tiempo.

  2. Redes Inalámbricas 802.11n el Nuevo Estándar

    OpenAIRE

    Héctor de Jesús Carlos Pérez; Karla Rocío Galván Salazar

    2006-01-01

    Existen varias compañías que se encuentran trabajando juntas para poder desarrollar el nuevo estándar llamado 802.11n. El estándar 802.11n será compatible con los estándares anteriores y contará con una mayor velocidad que las redes inalámbricas existentes, en donde se podrá transmitir video de alta calidad. Se espera que esta tecnología salga para el 2007.

  3. Relatório de Estágio Curricular – Gesto Apreciativo, Lda (Guarda)

    OpenAIRE

    Lino, Cristina

    2012-01-01

    Este Relatório resulta do Estágio Curricular realizado na empresa de consultoria e formação profissional, Gesto Apreciativo, Lda, na Guarda, onde foram realizadas tarefas nos vários departamentos da empresa, e que se enquadram no âmbito da Licenciatura em Gestão de Recursos Humanos da Escola Superior de Tecnologia e Gestão do Instituto Politécnico da Guarda. Durante as 400 horas em que decorreu o Estágio, foram desenvolvidas diversas atividades, quer na área de Recursos Humanos, quer noutr...

  4. Database Description - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us ClEST Database Description General information of database Database name ClEST Alternative n... Tsukuba, Ibaraki 305-8566 Japan Tel: +81-29-861-6812 (ext.222-36812) Fax: +81-29-861-6812 E-mail: Database ...classification Nucleotide Sequence Databases Organism Taxonomy Name: Cimex lectul...arius Taxonomy ID: 79782 Database description Expressed sequence tags (EST) database of unique organs and wh...ole bodies of the bedbug, Cimex lectularius Features and manner of utilization of database The bedbug Cimex

  5. LE CARCINOME ONCOCYTAIRE DE LA PAROTIDE : A PROPOS D ...

    African Journals Online (AJOL)

    Après chirurgie, les récidives sont fréquentes. Le rôle de la radio- thérapie et de chimiothérapie est toujours controversé.Le pronostic est mauvais. Mots clés : Carcinome oncocytaire, parotide, chirurgie, radiothérapie. SUMMARY. Introduction : Oncocyticcarcinomais a rare salivary gland tumor. its management is not codified ...

  6. Algunas consideraciones analíticas sobre el estándar de vida

    Directory of Open Access Journals (Sweden)

    Luis Fernando Gamboa

    2010-05-01

    Full Text Available El presente documento muestra las dificultades teóricas de la identificación del bienestar individual y las ventajas de interpretar el Índice de Condiciones de Vida (ICV y el Índice del Sistema de Selección de Beneficiarios (I-sisben como indicadores de estándar de vida. Se discuten el concepto de bienestar individual, tal como se concibe en la teoría económica convencional y en la teoría de Sen, y el concepto de estándar de vida. Se presentan dos lecturas que ha recibido el ICV: como un indicador de utilidad y como un indicador de estándar de vida. como resultado se recomienda leer el ICV como un indicador de estándar de vida, puesto que es una visión menos simplista que la tradicional

  7. El arte en el umbral. Estética y cultura del mercado

    Directory of Open Access Journals (Sweden)

    CARLOS FAJARDO FAJARDO

    2009-01-01

    Full Text Available Esta conferencia se centra en algunos aspectos donde creemos observar ciertas transformaciones importantes e ineludibles en la estética contemporánea. De este modo nos interesa indagar sobre las condiciones del arte y de las sensibilidades bajo los macroproyectos del mercado y de los medios, como también la mutación en algunas categorías de la estética moderna, tales como el concepto de obra de arte, el gusto estético, lo sublime, las relaciones entre arte y diseño. No es su propósito el rastrear la densa y exhaustiva historia de dichas mutaciones, más bien hemos realizado una mirada general a sus características actuales, mostrando algunos aspectos que operan en su interior.

  8. Restauraciones Estéticas en el Sector Anterior con Resina Compuesta. Reporte de un Caso.

    Directory of Open Access Journals (Sweden)

    Yecenia Perdomo Pacheco

    2011-10-01

    Full Text Available Las carillas directas se están convirtiendo en una solución para casi todos los problemas estéticos de la odontología actual. Los nuevos materiales de resina compuesta aparecidos en los últimos años han mejorado de forma importante sus propiedades mecánicas y estéticas, sobre todo la estabilidad del color, la textura y el pulido. Es por eso que cuando un paciente tiene una sonrisa con pequeñas alteraciones dentales, el uso de tratamientos conservadores tales como recontorneado gingival y/o gingivoplastia, blanqueamiento dental y restauraciones en resina, proporcionan un tratamiento predecible, con un resultado estético duradero, a un costo moderado.

  9. L'etat, C'est Moi: Why provincial Intra-governmental disputes in ...

    African Journals Online (AJOL)

    L'etat, C'est Moi: Why provincial Intra-governmental disputes in Shout Africa remain ungoverned by the final constitution and the Intergovernmental Relations Framework Act - and how we can best resolve them.

  10. [Development of new SSR markers from EST of SSH cDNA libraries on rose fragrance].

    Science.gov (United States)

    Yan, Hui-Jun; Zhang, Hao; Xie, Ji-Rong; Li, Shu-Fa; Jian, Hong-Ying; Qiu, Xian-Qin; Wang, Qi-Gang; Wang, Ji-Hua; Tang, Kai-Xue

    2009-09-01

    The new SSR markers of rose related fragrance were developed based on the SSH cDNA libraries of rose floral scent mutant. In this study, 10 EST-SSRs (2.6%) from 391 ESTs in the libraries were identified. Six EST-SSRs primers were designed to sequence flanking SSRs. The primer pairs designed were screened on the wild-type Jinyindao, which has flowers full of pleasant scent, and the mutant-type Wangriqinghuai without perceivable floral scent. Five primer pairs were amplified effectively in Jinyindao and Wangriqinghuai, and 3 were polymorphic between Jinyindao and Wangriqinghuai. Eighteen rose cultivars including fragrant roses and nonfragrant roses were identified by the five prime pairs. These results proved that EST-SSR markers are effective markers to identify the polymorphism of the rose.

  11. Composição arquitetônica e qualidade estética

    Directory of Open Access Journals (Sweden)

    Antônio Tarcísio da Luz Reis

    Full Text Available Este artigo analisa a relação entre composição arquitetônica e qualidade estética, considerando os pressupostos da estética filosófica e da estética empírica, e o papel da estética formal e da estética simbólica em explicar tal relação. Edificações históricas e contemporâneas com distintos níveis de ordem e estímulo visual são avaliadas por pessoas com distintos níveis e tipos de formação acadêmica e são identificadas as razões para tais avaliações. A coleta de dados inclui levantamentos de arquivo, levantamentos físicos, questionários e entrevistas realizados com 60 arquitetos, 60 não arquitetos com curso universitário e 60 pessoas sem curso universitário, que avaliaram imagens de três edificações históricas e três edificações contemporâneas, categorizadas conforme segue: ordem e estímulo visual; ordem e pouco estímulo visual; e desordem. As respostas dos questionários foram analisadas através de testes estatísticos não paramétricos, tais como Kruskal-Wallis e Kendall W. As informações fornecidas através das entrevistas foram analisadas por meio de suas frequências e significados. Os resultados indicam, que, embora os arquitetos tendam a valorizar a existência de ordem na composição arquitetônica e os não arquitetos a valorizar a existência de estímulo visual, quando ambos estão presentes a composição arquitetônica tende a ser avaliada positivamente.

  12. EST analysis in Ginkgo biloba: an assessment of conserved developmental regulators and gymnosperm specific genes.

    Science.gov (United States)

    Brenner, Eric D; Katari, Manpreet S; Stevenson, Dennis W; Rudd, Stephen A; Douglas, Andrew W; Moss, Walter N; Twigg, Richard W; Runko, Suzan J; Stellari, Giulia M; McCombie, W R; Coruzzi, Gloria M

    2005-10-15

    Ginkgo biloba L. is the only surviving member of one of the oldest living seed plant groups with medicinal, spiritual and horticultural importance worldwide. As an evolutionary relic, it displays many characters found in the early, extinct seed plants and extant cycads. To establish a molecular base to understand the evolution of seeds and pollen, we created a cDNA library and EST dataset from the reproductive structures of male (microsporangiate), female (megasporangiate), and vegetative organs (leaves) of Ginkgo biloba. RNA from newly emerged male and female reproductive organs and immature leaves was used to create three distinct cDNA libraries from which 6,434 ESTs were generated. These 6,434 ESTs from Ginkgo biloba were clustered into 3,830 unigenes. A comparison of our Ginkgo unigene set against the fully annotated genomes of rice and Arabidopsis, and all available ESTs in Genbank revealed that 256 Ginkgo unigenes match only genes among the gymnosperms and non-seed plants--many with multiple matches to genes in non-angiosperm plants. Conversely, another group of unigenes in Gingko had highly significant homology to transcription factors in angiosperms involved in development, including MADS box genes as well as post-transcriptional regulators. Several of the conserved developmental genes found in Ginkgo had top BLAST homology to cycad genes. We also note here the presence of ESTs in G. biloba similar to genes that to date have only been found in gymnosperms and an additional 22 Ginkgo genes common only to genes from cycads. Our analysis of an EST dataset from G. biloba revealed genes potentially unique to gymnosperms. Many of these genes showed homology to fully sequenced clones from our cycad EST dataset found in common only with gymnosperms. Other Ginkgo ESTs are similar to developmental regulators in higher plants. This work sets the stage for future studies on Ginkgo to better understand seed and pollen evolution, and to resolve the ambiguous phylogenetic

  13. Excitatriz estática en base al microcontrolador Intel 8751

    OpenAIRE

    Rivera Gaibor, Juan Carlos; Ledesma Galindo, Bolívar

    1993-01-01

    Se presenta la descripción y características de una excitatriz estática de 4.8 KW realizada en base al microcontrolador Intel 8751, el diseño del conversor estático utilizado, los circuitos de medición, y el software desarrollado para el control del conversor con tiristores y para la regulación del voltaje generado.

  14. José Antonio Ocampo : l'inclusion sociale et économique est ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    14 oct. 2015 ... Le développement rural est essentiel à la paix en Colombie, compte tenu du fait que le conflit qui perdure est principalement à caractère rural, a déclaré l'éminent économiste du développement, José Antonio Ocampo, lors d'une allocution qui a attiré un grand nombre de personnes, à Ottawa, le 25 ...

  15. Soutenir des politiques d'alimentation plus saine en Asie du Sud-Est ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Soutenir des politiques d'alimentation plus saine en Asie du Sud-Est. Près d'un quart des décès causés par des maladies non transmissibles dans le monde surviennent en Asie du Sud-Est. Ce projet tentera de résoudre le problème en menant des recherches sur la façon dont les politiques peuvent favoriser des ...

  16. En Asie du Sud-Est, la recherche en écosanté contribue à la ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    29 avr. 2016 ... C'est en Thaïlande que l'on observe la plus forte incidence de cholangiocarcinome, forme mortelle de cancer du foie notamment attribuable à une infection parasitaire par l'opisthorchiase (Opisthorchis viverrini). À certains endroits du nord-est de la Thaïlande, près de 85 % des habitants sont infectés par ...

  17. Two EST-derived marker systems for cultivar identification in tree peony.

    Science.gov (United States)

    Zhang, J J; Shu, Q Y; Liu, Z A; Ren, H X; Wang, L S; De Keyser, E

    2012-02-01

    Tree peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree peony expressed sequence tag (EST) database. Using target region amplification polymorphism (TRAP), 19 of 39 primer pairs showed good amplification for 56 accessions with amplicons ranging from 120 to 3,000 bp long, among which 99.3% were polymorphic. In contrast, 7 of 21 primer pairs demonstrated adequate amplification with clear bands for simple sequence repeats (SSRs) developed from ESTs, and a total of 33 alleles were found in 56 accessions. The similarity matrices generated by TRAP and EST-SSR markers were compared, and the Mantel test (r = 0.57778, P = 0.0020) showed a moderate correlation between the two types of molecular markers. TRAP markers were suitable for DNA fingerprinting and EST-SSR markers were more appropriate for discriminating synonyms (the same cultivars with different names due to limited information exchanged among different geographic areas). The two sets of EST-derived markers will be used further for genetic linkage map construction and quantitative trait locus detection in tree peony.

  18. Fronteras imaginadas y estereotipos : la creación de un monstruo llamado Europa del Este

    Directory of Open Access Journals (Sweden)

    Daniel Esparza

    2009-01-01

    Full Text Available En este artículo se observa como se han formado en el «oeste», desde la Guerra Fría hasta hoy, algunos estereotipos negativos sobre la marca «Europa del Este», la cual está cargada de connotaciones negativas (ciudades grises, guerras, inmigración ilegal, pobreza o criminalidad. Al mismo tiempo, como si de un oasis se tratara, se observa parojicamente como la marca Praga o Budapest (capitales de países de «Europa del Este» no son percibidas negativamente, precisamente porque no están relacionadas directamente con el Este, sino con centroeuropa y con imágenes positivas asociadas a la herencia artístico-cultural de los imperios centrales: el sacro imperio germano-romano y el austrohúngaro.In this article it is observed how from the Cold War till today have been formed in the West significant stereotypes towards Eastern Europe, which are endowed with negative images, such as grey towns, illegal immigration, wars, poverty or criminality. At the same time it is observed, paradoxically, how the brand Prague or Budapest (capitals of «Eastern European» countries are not perceived negatively, precisely because they are not directly associated with the East, but with the cultural heritage form the old central empires: the Sacred Roman-German empire first, and the Habsburg empire later.

  19. Update of the Diatom EST Database: a new tool for digital transcriptomics

    Science.gov (United States)

    Maheswari, Uma; Mock, Thomas; Armbrust, E. Virginia; Bowler, Chris

    2009-01-01

    The Diatom Expressed Sequence Tag (EST) Database was constructed to provide integral access to ESTs from these ecologically and evolutionarily interesting microalgae. It has now been updated with 130 000 Phaeodactylum tricornutum ESTs from 16 cDNA libraries and 77 000 Thalassiosira pseudonana ESTs from seven libraries, derived from cells grown in different nutrient and stress regimes. The updated relational database incorporates results from statistical analyses such as log-likelihood ratios and hierarchical clustering, which help to identify differentially expressed genes under different conditions, and allow similarities in gene expression in different libraries to be investigated in a functional context. The database also incorporates links to the recently sequenced genomes of P. tricornutum and T. pseudonana, enabling an easy cross-talk between the expression pattern of diatom orthologs and the genome browsers. These improvements will facilitate exploration of diatom responses to conditions of ecological relevance and will aid gene function identification of diatom-specific genes and in silico gene prediction in this largely unexplored class of eukaryotes. The updated Diatom EST Database is available at http://www.biologie.ens.fr/diatomics/EST3. PMID:19029140

  20. Development of Novel Polymorphic EST-SSR Markers in Bailinggu (Pleurotus tuoliensis for Crossbreeding

    Directory of Open Access Journals (Sweden)

    Yueting Dai

    2017-11-01

    Full Text Available Identification of monokaryons and their mating types and discrimination of hybrid offspring are key steps for the crossbreeding of Pleurotus tuoliensis (Bailinggu. However, conventional crossbreeding methods are troublesome and time consuming. Using RNA-seq technology, we developed new expressed sequence tag-simple sequence repeat (EST-SSR markers for Bailinggu to easily and rapidly identify monokaryons and their mating types, genetic diversity and hybrid offspring. We identified 1110 potential EST-based SSR loci from a newly-sequenced Bailinggu transcriptome and then randomly selected 100 EST-SSRs for further validation. Results showed that 39, 43 and 34 novel EST-SSR markers successfully identified monokaryons from their parent dikaryons, differentiated two different mating types and discriminated F1 and F2 hybrid offspring, respectively. Furthermore, a total of 86 alleles were detected in 37 monokaryons using 18 highly informative EST-SSRs. The observed number of alleles per locus ranged from three to seven. Cluster analysis revealed that these monokaryons have a relatively high level of genetic diversity. Transfer rates of the EST-SSRs in the monokaryons of closely-related species Pleurotus eryngii var. ferulae and Pleurotus ostreatus were 72% and 64%, respectively. Therefore, our study provides new SSR markers and an efficient method to enhance the crossbreeding of Bailinggu and closely-related species.

  1. New functions of the este system - new possibilities for emergency response

    International Nuclear Information System (INIS)

    Carny, P.

    2005-01-01

    The ESTE system (Emergency Source Term Evaluation) is support instrument for off-site emergency response and its main objective is to assist to the crisis staff: - to mitigate radiological consequences of significant releases; - to manage the protective measures; - to manage emergency monitoring. At national level the ESTE system are implemented at the Emergency Response Centre of the Czech Republic (SUJB) and Austrian versions are implemented at the Crisis Centre of the Austrian Republic (BMLFUW). ESTE system can now be utilized not only in close (40 km) vicinity of the point of the release (NPP), but radiological impacts are now calculated across the whole country or over the country border. Puff Trajectory Model (PTM) with the background of geographical information system (GIS) is included in este. Numerical weather prediction data (wind fields) predicted for the whole or the part of the country are online connected with este and utilized for the puffs movement simulation and impacts calculations. It means that not only meteorological data from the point of release (measured or predicted), but 'meteorological data wind field' predicted for larger region across the country are used by the este system. (author)

  2. Generation and analysis of expressed sequence tags (ESTs) for marker development in yam (Dioscorea alata L.)

    Science.gov (United States)

    2011-01-01

    Background Anthracnose (Colletotrichum gloeosporioides) is a major limiting factor in the production of yam (Dioscorea spp.) worldwide. Availability of high quality sequence information is necessary for designing molecular markers associated with resistance. However, very limited sequence information pertaining to yam is available at public genome databases. Therefore, this collaborative project was developed for genetic improvement and germplasm characterization of yams using molecular markers. The current investigation is focused on studying gene expression, by large scale generation of ESTs, from one susceptible (TDa 95-0310) and two resistant yam genotypes (TDa 87-01091, TDa 95-0328) challenged with the fungus. Total RNA was isolated from young leaves of resistant and susceptible genotypes and cDNA libraries were sequenced using Roche 454 technology. Results A total of 44,757 EST sequences were generated from the cDNA libraries of the resistant and susceptible genotypes. Greater than 56% of ESTs were annotated using MapMan Mercator tool and Blast2GO search tools. Gene annotations were used to characterize the transcriptome in yam and also perform a differential gene expression analysis between the resistant and susceptible EST datasets. Mining for SSRs in the ESTs revealed 1702 unique sequences containing SSRs and 1705 SSR markers were designed using those sequences. Conclusion We have developed a comprehensive annotated transcriptome data set in yam to enrich the EST information in public databases. cDNA libraries were constructed from anthracnose fungus challenged leaf tissues for transcriptome characterization, and differential gene expression analysis. Thus, it helped in identifying unique transcripts in each library for disease resistance. These EST resources provide the basis for future microarray development, marker validation, genetic linkage mapping and QTL analysis in Dioscorea species. PMID:21303556

  3. Generation and analysis of expressed sequence tags (ESTs for marker development in yam (Dioscorea alata L.

    Directory of Open Access Journals (Sweden)

    Robert Asiedu

    2011-02-01

    Full Text Available Abstract Background Anthracnose (Colletotrichum gloeosporioides is a major limiting factor in the production of yam (Dioscorea spp. worldwide. Availability of high quality sequence information is necessary for designing molecular markers associated with resistance. However, very limited sequence information pertaining to yam is available at public genome databases. Therefore, this collaborative project was developed for genetic improvement and germplasm characterization of yams using molecular markers. The current investigation is focused on studying gene expression, by large scale generation of ESTs, from one susceptible (TDa 95-0310 and two resistant yam genotypes (TDa 87-01091, TDa 95-0328 challenged with the fungus. Total RNA was isolated from young leaves of resistant and susceptible genotypes and cDNA libraries were sequenced using Roche 454 technology. Results A total of 44,757 EST sequences were generated from the cDNA libraries of the resistant and susceptible genotypes. Greater than 56% of ESTs were annotated using MapMan Mercator tool and Blast2GO search tools. Gene annotations were used to characterize the transcriptome in yam and also perform a differential gene expression analysis between the resistant and susceptible EST datasets. Mining for SSRs in the ESTs revealed 1702 unique sequences containing SSRs and 1705 SSR markers were designed using those sequences. Conclusion We have developed a comprehensive annotated transcriptome data set in yam to enrich the EST information in public databases. cDNA libraries were constructed from anthracnose fungus challenged leaf tissues for transcriptome characterization, and differential gene expression analysis. Thus, it helped in identifying unique transcripts in each library for disease resistance. These EST resources provide the basis for future microarray development, marker validation, genetic linkage mapping and QTL analysis in Dioscorea species.

  4. Characterization and development of EST-SSR markers in sweet potato (Ipomoea batatas (L.) Lam).

    Science.gov (United States)

    Kim, Jin-Hee; Kim, Jun-Hoi; Jo, Won-Sam; Ham, Jeong-Gwan; Chung, Il Kyung; Kim, Kyung-Min

    2016-12-01

    In this study, a cDNA library was constructed from the total RNA of sweet potato leaves. A total of 789 copies of the cDNA were cloned in Escherichia coli by employing the pGEM-T Easy vector. Sequencing was carried out by Solgent Co. (Korea). As many as 579 expressed sequence tag-simple sequence repeat (EST-SSR) markers were designed (73.38%) from the known cDNA nucleotide base sequences. The lengths of the developed EST-SSR markers ranged from 100 to 499 bp (average length 238 bp). Their motif sequence types were varied, with most being dinucleotides and pentanucleotides, and the most commonly found motifs were CAGAAT (29.0%) and TCT (2.8%). Based on these SSR-containing sequences, 619 pairs of high-quality SSR primers were designed using WebSat and Primer3web. The total number of primers designed was 144. Polymorphism was evident in 82 EST-SSR markers among 20 Korean sweet potato cultivars tested and in 90 EST-SSR markers in the two parents of a mapping population, Yeseumi and Annobeny. In this study, the hexaploid sweet potato (2n = 6x = 90) EST-SSR markers were developed in the absence of full-sequence data. Moreover, by acting as a molecular tag for particular traits, the EST-SSR marker can also simultaneously identify information about the corresponding gene. These EST-SSR markers will allow the molecular analysis of sweet potato to be done more efficiently. Thus, we can develop high-quality sweet potato while overcoming the challenges from climate change and other unfavorable conditions.

  5. Formação de classes funcionais de estímulos musicais

    Directory of Open Access Journals (Sweden)

    Alex Roberto Machado

    2009-04-01

    Full Text Available O objetivo deste experimento foi verificar o efeito do treino discriminativo sobre a formação de classes funcionais de melodias em andamentos e modos diferentes. Nove estudantes universitários do 2° período da graduação em Psicologia foram divididos em três grupos: Modo, Andamento ou Misto. O software SomPsi, produzido especialmente para este estudo, treinou os participantes e gerou relatórios com informações sobre os desempenhos, nas quatro fases do procedimento. O treino proposto mostrou-se eficiente para a formação das classes funcionais de estímulos sonoros musicais. Observou-se que agrupamentos de estímulos pela propriedade andamento foram mais facilmente discriminados que os agrupados pela melodia. A manipulação combinada das duas propriedades, em condição convergente, permitiu desempenho superior dos participantes. A rápida formação de classes funcionais e equivalentes de estímulos musicais, a partir de procedimentos de ensino automatizado de relações condicionais entre estímulos musicais, indica a possibilidade de uso desse procedimento na iniciação à música.

  6. Hacia una estética de las nuevas tecnologías como proyecto educativo

    Directory of Open Access Journals (Sweden)

    Juan Sebastián López

    2011-01-01

    Full Text Available Este artículo parte de la descripción de un cambio de época en curso, analiza - do en los niveles del orden social, la cultura, la comunicación y las tecnologías. De ahí se extraen una serie de retos a los que se enfrenta cualquier proyecto de educación contemporáneo. En este marco problémico, se examinarán algunos puntos fundamentales de la teoría estética de Theodor Adorno. Lo que se intenta proponer es una concepción de la estética asumida como mediación conceptual de la experiencia del sujeto con su entorno. Este será el primer paso para consolidar un proyecto educativo que resuelva uno de sus retos más urgentes: reducir la brecha entre el desarrollo tecnológico y sus correspondientes mediaciones culturales.

  7. Estética fenomenológica. La obra de arte arquitectónica

    Directory of Open Access Journals (Sweden)

    Pedragosa, Pau

    2009-04-01

    Full Text Available The purpose of this article is to discuss the possibility and task of aesthetics under the phenomenological point of view. I focus on architecture and analyse its stratified structure, according to Roman Ingarden’s analysis, and the specific character of the aesthetic attitude against other attitudes that architecture also demands, according to the observations of Paul Valéry. I finally raise the central question about the possibility of the understanding art in general and architecture in particular, comparing phenomenological aesthetics and aesthetics of negativity.El propósito de este artículo es discutir la posibilidad y tarea de la estética bajo un enfoque fenomenológico. Nos centramos en la arquitectura, de la cual analizamos su estructura estratificada, siguiendo los análisis de Roman Ingarden, y la especificidad de la actitud estética, frente a otras actitudes que también reclama la arquitectura, según las observaciones de Paul Valéry. Planteamos finalmente la cuestión central acerca de la posibilidad de la comprensión del arte en general y de la arquitectura en particular comparando la estética fenomenológica con la estética de la negatividad.

  8. Developing new SSR markers from ESTs of pea (Pisum sativum L.).

    Science.gov (United States)

    Gong, Ya-ming; Xu, Sheng-chun; Mao, Wei-hua; Hu, Qi-zan; Zhang, Gu-wen; Ding, Ju; Li, Ya-dan

    2010-09-01

    The development of expressed sequence tags (ESTs) from pea has provided a useful source for mining novel simple sequence repeat (SSR) markers. In the present research, in order to find EST-derived SSR markers, 18 552 pea ESTs from the National Center for Biotechnology Information (NCBI) database were downloaded and assembled into 10 086 unigenes. A total of 586 microsatellites in 530 unigenes were identified, indicating that merely 5.25% of sequences contained SSRs. The most abundant SSRs within pea were tri-nucleotide repeat motifs, and among all the tri-nucleotide repeats, the motif GAA was the most abundant type. In total, 49 SSRs were used for primer design. EST-SSR loci were subsequently screened on 10 widely adapted varieties in China. Of these, nine loci showed polymorphic profiles that revealed two to three alleles per locus. The polymorphism information content value ranged from 0.18 to 0.58 with an average of 0.41. Furthermore, transferable analysis revealed that some of these loci showed transferability to faba bean. Because of their polymorphism and transferability, these nine novel EST-SSRs will be valuable tools for marker-assisted breeding and comparative mapping of pea in the future.

  9. Impact d'une hyperhomocystéinémie sur les lipoprotéines ...

    African Journals Online (AJOL)

    hyperhomocystéinémie est associée aux maladies cardiovasculaires. Nous avons également mis en évidence, cette association chez le Rat des sables, Psammomys obesus. Un état d‟hyperhomocystéinémie est installé par une administration chronique ...

  10. Los patrones estéticos en los albores del siglo XXI. Hacia una revisión de los estudios en torno a este tema

    Directory of Open Access Journals (Sweden)

    CARMEN BAÑUELOS

    1994-01-01

    Full Text Available El culto del cuerpo se ha convertido en nuestros días en un fenómeno social de gran significación. La presentación del yo físico adquiere una especial relevancia en los nuevos estilos de vida. Diversos grupos sociales (médicos, psicologos, sociólogos, modistos, publicistas, estilistas, esteticistas, periodistas... centran su interés en la definición social del "cuerpo". el objetivo es contribuir a la creación de un modelo ideal único y generalizable, legitimando determinadas prácticas sociales dirigidas hacia este fín. La definición de este modelo esta en manos de un grupo de "diseñadores de cuerpos" que asumen el papel de especificar los límites de la normalidad corporal en nuestra sociedad. La expresión corporal se ha convertido en un lenguaje que, en último extremo, manifiesta el status y la posición social. Por otra parte, en las últimas décadas se plantea la cuestión de conocer como las diferencias biológicas entre los individuos se transforman en factores de discriminación social. En este sentido, diversos problemas de salud actuales chocan con la definición de "cuerpo" ideal. La gordura es un buen ejemplo para comprender como las modas sociales utilizan la categorización de enfermedad "etiquetando" lo "normal" en un momento dado como "patológico" en otro. La exclusión social de las personas obesas, el rechazo a la obesidad y la construcción social de su figura marginal correspondiente, adquieren una relevancia merecedora de un análisis sociológico mas exhaustivo. Por este motivo, es necesario comprender como se han impuesto criterios sociales para estigmatizar a personas cuyo peso no es un riesgo para la salud ni un obstaculo para su vida social...

  11. Needles in the EST haystack: large-scale identification and analysis of excretory-secretory (ES proteins in parasitic nematodes using expressed sequence tags (ESTs.

    Directory of Open Access Journals (Sweden)

    Shivashankar H Nagaraj

    2008-09-01

    Full Text Available Parasitic nematodes of humans, other animals and plants continue to impose a significant public health and economic burden worldwide, due to the diseases they cause. Promising antiparasitic drug and vaccine candidates have been discovered from excreted or secreted (ES proteins released from the parasite and exposed to the immune system of the host. Mining the entire expressed sequence tag (EST data available from parasitic nematodes represents an approach to discover such ES targets.In this study, we predicted, using EST2Secretome, a novel, high-throughput, computational workflow system, 4,710 ES proteins from 452,134 ESTs derived from 39 different species of nematodes, parasitic in animals (including humans or plants. In total, 2,632, 786, and 1,292 ES proteins were predicted for animal-, human-, and plant-parasitic nematodes. Subsequently, we systematically analysed ES proteins using computational methods. Of these 4,710 proteins, 2,490 (52.8% had orthologues in Caenorhabditis elegans, whereas 621 (13.8% appeared to be novel, currently having no significant match to any molecule available in public databases. Of the C. elegans homologues, 267 had strong "loss-of-function" phenotypes by RNA interference (RNAi in this nematode. We could functionally classify 1,948 (41.3% sequences using the Gene Ontology (GO terms, establish pathway associations for 573 (12.2% sequences using Kyoto Encyclopaedia of Genes and Genomes (KEGG, and identify protein interaction partners for 1,774 (37.6% molecules. We also mapped 758 (16.1% proteins to protein domains including the nematode-specific protein family "transthyretin-like" and "chromadorea ALT," considered as vaccine candidates against filariasis in humans.We report the large-scale analysis of ES proteins inferred from EST data for a range of parasitic nematodes. This set of ES proteins provides an inventory of known and novel members of ES proteins as a foundation for studies focused on understanding the

  12. Needles in the EST Haystack: Large-Scale Identification and Analysis of Excretory-Secretory (ES) Proteins in Parasitic Nematodes Using Expressed Sequence Tags (ESTs)

    Science.gov (United States)

    Nagaraj, Shivashankar H.; Gasser, Robin B.; Ranganathan, Shoba

    2008-01-01

    Background Parasitic nematodes of humans, other animals and plants continue to impose a significant public health and economic burden worldwide, due to the diseases they cause. Promising antiparasitic drug and vaccine candidates have been discovered from excreted or secreted (ES) proteins released from the parasite and exposed to the immune system of the host. Mining the entire expressed sequence tag (EST) data available from parasitic nematodes represents an approach to discover such ES targets. Methods and Findings In this study, we predicted, using EST2Secretome, a novel, high-throughput, computational workflow system, 4,710 ES proteins from 452,134 ESTs derived from 39 different species of nematodes, parasitic in animals (including humans) or plants. In total, 2,632, 786, and 1,292 ES proteins were predicted for animal-, human-, and plant-parasitic nematodes. Subsequently, we systematically analysed ES proteins using computational methods. Of these 4,710 proteins, 2,490 (52.8%) had orthologues in Caenorhabditis elegans, whereas 621 (13.8%) appeared to be novel, currently having no significant match to any molecule available in public databases. Of the C. elegans homologues, 267 had strong “loss-of-function” phenotypes by RNA interference (RNAi) in this nematode. We could functionally classify 1,948 (41.3%) sequences using the Gene Ontology (GO) terms, establish pathway associations for 573 (12.2%) sequences using Kyoto Encyclopaedia of Genes and Genomes (KEGG), and identify protein interaction partners for 1,774 (37.6%) molecules. We also mapped 758 (16.1%) proteins to protein domains including the nematode-specific protein family “transthyretin-like” and “chromadorea ALT,” considered as vaccine candidates against filariasis in humans. Conclusions We report the large-scale analysis of ES proteins inferred from EST data for a range of parasitic nematodes. This set of ES proteins provides an inventory of known and novel members of ES proteins as a

  13. Desenvolvimento de competências gerais durante o estágio supervisionado

    OpenAIRE

    Gladys Amelia Vélez Benito; Kamila Medani Tristão; Ana Claúdia Schuab Faria de Paula; Mariana Andrade dos Santos; Lorena Jácome Ataide; Rita de Cássia Duarte Lima

    2012-01-01

    Este artigo descreve as competências gerais dos profissionais de saúde e aponta possíveis articulações com o estágio supervisionado. O objetivo é chamar a atenção dos enfermeiros e futuros profissionais da área, sobre a importância de se vivenciar o estágio supervisionado, durante a formação, para a aquisição e aprimoramento das competências gerais dos profissionais de saúde, além das competências e habilidades específicas da Enfermagem. Nos serviços de saúde, tanto na esfera pública quanto n...

  14. Identification and Characterization of MicroRNAs in Macaca fascicularis by EST Analysis

    Directory of Open Access Journals (Sweden)

    Hao Yang

    2012-01-01

    Full Text Available MicroRNAs (miRNAs are small noncoding RNAs which repress gene expression at the posttranscriptional level. In this study, an expressed sequence tag (EST-based combined method was applied for the detection of miRNAs in Macaca fascicularis which is used as a model animal extensively in medical experiments, particularly those involved with neuroscience and disease. Initially, previously known miRNA sequences from metazoans were used to blast with the EST databases of Macaca fascicularis, and then a range of filtering criteria was conducted to remove some pseudo ones. At last a total of 8 novel conserved miRNAs were identified; their functions were further predicted and analyzed. Together, our study provides insight into miRNAs and their functions in Macaca fascicularis, indicating that the EST analysis is an efficient and affordable alternative approach for identifying novel miRNA candidates.

  15. Los saberes del arte. LA experiencia estética en Nietzsche

    Directory of Open Access Journals (Sweden)

    Luis Eduardo Gama

    2008-01-01

    sobre el arte pueda tener al interior de la esfera estética misma: ¿qué es lo específico del arte frente a otras esferas de la acción humana? ¿En qué consiste propiamente la experiencia estética? ¿Qué resulta para el individuo y para la cultura del encuentro con el fenómeno del arte? El presente artículo constituye un primer intento de responder estas cuestiones. Su base de análisis se concentra en El nacimiento de la tragedia, y en menor medida en la fisiología del arte del Crepúsculo de los ídolos. La tesis central que se defiende es que la experiencia estética para Nietzsche es esencialmente una experiencia de conocimiento, de un tipo de saber que da acceso a la verdad.

  16. Pairagon+N-SCAN_EST: a model-based gene annotation pipeline

    DEFF Research Database (Denmark)

    Arumugam, Manimozhiyan; Wei, Chaochun; Brown, Randall H

    2006-01-01

    This paper describes Pairagon+N-SCAN_EST, a gene annotation pipeline that uses only native alignments. For each expressed sequence it chooses the best genomic alignment. Systems like ENSEMBL and ExoGean rely on trans alignments, in which expressed sequences are aligned to the genomic loci...... of putative homologs. Trans alignments contain a high proportion of mismatches, gaps, and/or apparently unspliceable introns, compared to alignments of cDNA sequences to their native loci. The Pairagon+N-SCAN_EST pipeline's first stage is Pairagon, a cDNA-to-genome alignment program based on a Pair......HMM probability model. This model relies on prior knowledge, such as the fact that introns must begin with GT, GC, or AT and end with AG or AC. It produces very precise alignments of high quality cDNA sequences. In the genomic regions between Pairagon's cDNA alignments, the pipeline combines EST alignments...

  17. Dispositivos estéticos y formas sensibles de la emancipación

    Directory of Open Access Journals (Sweden)

    María Luciana Cadahia

    2016-08-01

    Full Text Available Se explora la noción de dispositivo desde una perspectiva estético-política. Se inquieren los motivos para distanciarnos de las lecturas de Agamben, Esposito y el Colectivo tiqqun,se examina la interpretación de Deleuze al respecto, en una dimensión poco conocida, y se amplía esta perspectiva hacia una concepción estética del dispositivo, atendiendo para ello a Martín-Barbero. Finalmente, se considera el vínculo problemático entre el dispositivo y la positividad estética, para acercarnos a Schiller, quien da las pautas para disolver el vínculo problemático.

  18. Estrabismo após toxina botulínica para fins estéticos

    Directory of Open Access Journals (Sweden)

    Marta Halfeld Ferrari Alves Lacordia

    2011-06-01

    Full Text Available RESUMO Nas últimas décadas, a toxina botulínica tem sido aplicada para fins terapêuticos em inúmeras afecções. A toxina do tipo A foi aprovada primeiramente em 1989 para o uso em estrabismo, blefaroespasmo e espasmo hemifacial de pacientes maiores que 12 anos de idade. A observação de que pacientes tratados de blefaroespasmo com toxina botulínica apresentavam um efeito adicional de diminuição das rugas de expressão inspirou estudos sobre a aplicação cosmética da toxina. Recentemente, a toxina botulínica do tipo A foi aprovada para fins estéticos. Este trabalho relata um caso de ocorrência de estrabismo após aplicação de toxina botulínica na face para fins estéticos.

  19. Efficient Serial and Parallel Algorithms for Selection of Unique Oligos in EST Databases.

    Science.gov (United States)

    Mata-Montero, Manrique; Shalaby, Nabil; Sheppard, Bradley

    2013-01-01

    Obtaining unique oligos from an EST database is a problem of great importance in bioinformatics, particularly in the discovery of new genes and the mapping of the human genome. Many algorithms have been developed to find unique oligos, many of which are much less time consuming than the traditional brute force approach. An algorithm was presented by Zheng et al. (2004) which finds the solution of the unique oligos search problem efficiently. We implement this algorithm as well as several new algorithms based on some theorems included in this paper. We demonstrate how, with these new algorithms, we can obtain unique oligos much faster than with previous ones. We parallelize these new algorithms to further improve the time of finding unique oligos. All algorithms are run on ESTs obtained from a Barley EST database.

  20. Cross-species EST alignments reveal novel and conserved alternative splicing events in legumes

    Directory of Open Access Journals (Sweden)

    Brendel Volker

    2008-02-01

    Full Text Available Abstract Background Although originally thought to be less frequent in plants than in animals, alternative splicing (AS is now known to be widespread in plants. Here we report the characteristics of AS in legumes, one of the largest and most important plant families, based on EST alignments to the genome sequences of Medicago truncatula (Mt and Lotus japonicus (Lj. Results Based on cognate EST alignments alone, the observed frequency of alternatively spliced genes is lower in Mt (~10%, 1,107 genes and Lj (~3%, 92 genes than in Arabidopsis and rice (both around 20%. However, AS frequencies are comparable in all four species if EST levels are normalized. Intron retention is the most common form of AS in all four plant species (~50%, with slightly lower frequency in legumes compared to Arabidopsis and rice. This differs notably from vertebrates, where exon skipping is most common. To uncover additional AS events, we aligned ESTs from other legume species against the Mt genome sequence. In this way, 248 additional Mt genes were predicted to be alternatively spliced. We also identified 22 AS events completely conserved in two or more plant species. Conclusion This study extends the range of plant taxa shown to have high levels of AS, confirms the importance of intron retention in plants, and demonstrates the utility of using ESTs from related species in order to identify novel and conserved AS events. The results also indicate that the frequency of AS in plants is comparable to that observed in mammals. Finally, our results highlight the importance of normalizing EST levels when estimating the frequency of alternative splicing.

  1. Psicologia Organizacional e do Trabalho: Relato de Experiência em Estágio Supervisionado

    Directory of Open Access Journals (Sweden)

    Priscilla de Oliveira Martins-Silva

    Full Text Available Resumo Trata-se de relato de experiência em estágio supervisionado em Psicologia Organizacional e do Trabalho (POT envolvendo um grupo de alunos de uma Instituição de Educação Superior (IES localizada no Estado do Espírito Santo. O objetivo central foi discutir os aspectos teóricos e práticos que influenciaram na formação profissional do discente em Psicologia, tendo como foco o estágio supervisionado. Além de relatar a experiência, foi realizado um levantamento junto aos ex-alunos sobre as contribuições do estágio na sua formação educacional e profissional. Da experiência relatada foi possível verificar que: a instituição das Diretrizes Curriculares Nacionais (DCN constituiu-se em um marco na flexibilização curricular dos cursos; o estágio é um componente curricular de integração entre ensino, pesquisa e prática; e o estágio em POT requer dos discentes conhecimentos multidisciplinares. Além desses aspectos, observou-se que a prática do estágio supervisionado indica o rompimento com a imagem negativa do psicólogo que atua em POT, limitada à aplicação de testes e psicotécnicas. De fato, a atividade do psicólogo em POT mostrou-se muito mais ampla e complexa, sobretudo em termos dos impactos de sua intervenção no ambiente organizacional.

  2. Evaluación estética de seis tipos de coronas para dientes primarios

    Directory of Open Access Journals (Sweden)

    Héctor Alejandro Ramírez Peña

    2017-03-01

    Full Text Available Objetivo: Evaluar las preferencias estéticas en relación con el color y la forma de coronas primarias utilizadas para dientes incisivos superiores primarios, mediante la realización de una encuesta a miembros de la Academia Mexicana de Odontología Pediatrica (AMOP. Material y Métodos: Se establecieron seis grupos de estudio con seis coronas diferentes: grupo 1, coronas de zirconia EZ-Pedo; grupo 2, coronas de zirconia NuSmile Zr; grupo 3, coronas estéticas hechas en el consultorio; grupo 4, coronas de fundas de celuloide; grupo 5, coronas estéticas prefabricadas NuSmile signature; y grupo 6, coronas estéticas fenestradas. Se llevaron a cabo encuestas con la finalidad de conocer las preferencias estéticas de estas diferentes coronas, con la finalidad de conocer cuál es la mejor opción para su uso en el consultorio dental. Resultados: Noventa miembros de la AMOP realizaron una encuesta válida, y se determinó que el grupo 4 fue el mejor evaluado, seguido de los grupos 2, 5, 1, 6 y 3. Se identificaron diferencias significativas entre los diferentes grupos. Conclusiones: Las coronas de fundas de celuloide fueron seleccionadas como mejor alternativa de uso en los dientes primarios anteriores, por parte de los miembros de la AMOP; asimismo, se consideró a las coronas de zirconia como una buena opción terapéutica. Es recomendable que se implemente el tratamiento estético en dientes primarios, para realizar un tratamiento integral.

  3. La nueva Estética transcendental del Opus Postumum de Kant

    Directory of Open Access Journals (Sweden)

    Leopoldo Prieto López

    2014-11-01

    Full Text Available Este artículo presenta las razones de la transformación de la teoría kantiana de la sensilibilidad,es decir, de la estética trascendental. La estética trascendental de la Crítica de la razón pura contenía una clara aporía: por un lado, la cosa en sí era entendida como la causa de la sensación, pero, por otro, la categoría de causa no era de legítima aplicación a la cosa en sí. Este insatisfactorio estatuto epistemológico de la estética trascendental y de la afección sensible fue vivamente discutido y criticado, ya en tiempos de Kant, en lo que se conoce con el nombre de disputa en torno a la cosa en sí,en la que intervinieron, entre otros, Jacobi, Reinhold, Beck y Schelling. De este modo Kant, sea para hacer más coherente el propio sistema, sea incluso por ser de algún modo influenciado por tales críticas,llevó a cabo en el Opus Postumum una profunda reforma de la teoría de la sensibilidad, que se fundará sobre dos principios: la transformación de la vieja afección transcendente en la nueva teoría de la autoafección (Selbstaffizierung, y consecuentemente, la disolución de la cosa en sí dentro de la potencia sintética del yo, que ahora no es afectado por la cosa, sino por sí mismo

  4. Generation, annotation and analysis of ESTs from Trichoderma harzianum CECT 2413

    Directory of Open Access Journals (Sweden)

    Gutiérrez Santiago

    2006-07-01

    Full Text Available Abstract Background The filamentous fungus Trichoderma harzianum is used as biological control agent of several plant-pathogenic fungi. In order to study the genome of this fungus, a functional genomics project called "TrichoEST" was developed to give insights into genes involved in biological control activities using an approach based on the generation of expressed sequence tags (ESTs. Results Eight different cDNA libraries from T. harzianum strain CECT 2413 were constructed. Different growth conditions involving mainly different nutrient conditions and/or stresses were used. We here present the analysis of the 8,710 ESTs generated. A total of 3,478 unique sequences were identified of which 81.4% had sequence similarity with GenBank entries, using the BLASTX algorithm. Using the Gene Ontology hierarchy, we performed the annotation of 51.1% of the unique sequences and compared its distribution among the gene libraries. Additionally, the InterProScan algorithm was used in order to further characterize the sequences. The identification of the putatively secreted proteins was also carried out. Later, based on the EST abundance, we examined the highly expressed genes and a hydrophobin was identified as the gene expressed at the highest level. We compared our collection of ESTs with the previous collections obtained from Trichoderma species and we also compared our sequence set with different complete eukaryotic genomes from several animals, plants and fungi. Accordingly, the presence of similar sequences in different kingdoms was also studied. Conclusion This EST collection and its annotation provide a significant resource for basic and applied research on T. harzianum, a fungus with a high biotechnological interest.

  5. Signs of positive selection of somatic mutations in human cancers detected by EST sequence analysis

    International Nuclear Information System (INIS)

    Babenko, Vladimir N; Basu, Malay K; Kondrashov, Fyodor A; Rogozin, Igor B; Koonin, Eugene V

    2006-01-01

    Carcinogenesis typically involves multiple somatic mutations in caretaker (DNA repair) and gatekeeper (tumor suppressors and oncogenes) genes. Analysis of mutation spectra of the tumor suppressor that is most commonly mutated in human cancers, p53, unexpectedly suggested that somatic evolution of the p53 gene during tumorigenesis is dominated by positive selection for gain of function. This conclusion is supported by accumulating experimental evidence of evolution of new functions of p53 in tumors. These findings prompted a genome-wide analysis of possible positive selection during tumor evolution. A comprehensive analysis of probable somatic mutations in the sequences of Expressed Sequence Tags (ESTs) from malignant tumors and normal tissues was performed in order to access the prevalence of positive selection in cancer evolution. For each EST, the numbers of synonymous and non-synonymous substitutions were calculated. In order to identify genes with a signature of positive selection in cancers, these numbers were compared to: i) expected numbers and ii) the numbers for the respective genes in the ESTs from normal tissues. We identified 112 genes with a signature of positive selection in cancers, i.e., a significantly elevated ratio of non-synonymous to synonymous substitutions, in tumors as compared to 37 such genes in an approximately equal-sized EST collection from normal tissues. A substantial fraction of the tumor-specific positive-selection candidates have experimentally demonstrated or strongly predicted links to cancer. The results of EST analysis should be interpreted with extreme caution given the noise introduced by sequencing errors and undetected polymorphisms. Furthermore, an inherent limitation of EST analysis is that multiple mutations amenable to statistical analysis can be detected only in relatively highly expressed genes. Nevertheless, the present results suggest that positive selection might affect a substantial number of genes during

  6. annot8r: GO, EC and KEGG annotation of EST datasets.

    Science.gov (United States)

    Schmid, Ralf; Blaxter, Mark L

    2008-04-09

    The expressed sequence tag (EST) methodology is an attractive option for the generation of sequence data for species for which no completely sequenced genome is available. The annotation and comparative analysis of such datasets poses a formidable challenge for research groups that do not have the bioinformatics infrastructure of major genome sequencing centres. Therefore, there is a need for user-friendly tools to facilitate the annotation of non-model species EST datasets with well-defined ontologies that enable meaningful cross-species comparisons. To address this, we have developed annot8r, a platform for the rapid annotation of EST datasets with GO-terms, EC-numbers and KEGG-pathways. annot8r automatically downloads all files relevant for the annotation process and generates a reference database that stores UniProt entries, their associated Gene Ontology (GO), Enzyme Commission (EC) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) annotation and additional relevant data. For each of GO, EC and KEGG, annot8r extracts a specific sequence subset from the UniProt dataset based on the information stored in the reference database. These three subsets are then formatted for BLAST searches. The user provides the protein or nucleotide sequences to be annotated and annot8r runs BLAST searches against these three subsets. The BLAST results are parsed and the corresponding annotations retrieved from the reference database. The annotations are saved both as flat files and also in a relational postgreSQL results database to facilitate more advanced searches within the results. annot8r is integrated with the PartiGene suite of EST analysis tools. annot8r is a tool that assigns GO, EC and KEGG annotations for data sets resulting from EST sequencing projects both rapidly and efficiently. The benefits of an underlying relational database, flexibility and the ease of use of the program make it ideally suited for non-model species EST-sequencing projects.

  7. SEffEst: Effort estimation in software projects using fuzzy logic and neural networks

    Directory of Open Access Journals (Sweden)

    Israel

    2012-08-01

    Full Text Available Academia and practitioners confirm that software project effort prediction is crucial for an accurate software project management. However, software development effort estimation is uncertain by nature. Literature has developed methods to improve estimation correctness, using artificial intelligence techniques in many cases. Following this path, this paper presents SEffEst, a framework based on fuzzy logic and neural networks designed to increase effort estimation accuracy on software development projects. Trained using ISBSG data, SEffEst presents remarkable results in terms of prediction accuracy.

  8. La dimensión estética del poder mediático

    OpenAIRE

    Ximena González

    2009-01-01

    El poder de los medios de comunicación y información es, en la mayoría de los casos, analizado a partir de larelación comando-obediencia, es decir como poder coercitivo. Esto conlleva a centrarse en los efectos de dicho poder, yno en lo que hace la particularidad del poder mediático en la reestructuración de las sociedades actuales. Este artículoplantea caracterizar este poder mediático - multiplicado por el desarrollo masivo de las tecnologías de informacióny comunicación - ya no únicamente ...

  9. Entretelones de una "estética operatoria" Luis Juan Guerrero y Walter Benjamin

    OpenAIRE

    Luis Ignacio García

    2009-01-01

    En este artículo se ensaya una aproximación a la labor estético-filosófica del argentino Luis Juan Guerrero (1899-1957), a través de un cotejo de su temprana y productiva recepción de algunos aspectos centrales de la obra de Walter Benjamin. Se intenta mostrar que esta clave de lectura permite un acceso privilegiado a la labor de Guerrero, a la vez que ayuda a construir una imagen más compleja y matizada de la generación intelectual "antipositivista" a la que perteneció. La olvidada obra de G...

  10. Práticas de letramento docente no estágio supervisionado de letras estrangeiras

    OpenAIRE

    Reichmann, Carla Lynn

    2012-01-01

    Neste artigo discuto a relevância de práticas de letramento no estágio. Alinhando-me aos novos estudos de letramento (BARTON et al., 2000) e considerando a escrita como elemento identitário de formação (KLEIMAN, 2007), adoto a perspectiva bakhtiniana de gênero a fim de investigar relatos autobiográficos alicerçados em fotobiografias escolares produzidas em uma disciplina de estágio em língua estrangeira, em 2011. Neste recorte analiso as vozes enunciativas que ecoam em dois relatos, em especi...

  11. Budismo Zen: repercusiones estéticas en Oriente y Occidente

    OpenAIRE

    Villalba Fernández, Javier

    2011-01-01

    En la presente tesis se aborda el estudio del movimiento o corriente budista de Meditación (conocido como Ch’an en China, Sŏn en Corea y Zen en Japón) y se analizan las repercusiones estéticas de la doctrina de este tipo de budismo. En la primera parte (Budismo Zen) se revisan de los antecedentes filosóficos, religiosos e históricos, y se analizan los desarrollos doctrinales e institucionales de esta corriente “de meditación” del budismo, todo lo cual resulta esencial para valorar apropiadame...

  12. Comparación entre varias poblaciones estándar para el ajuste por edades

    OpenAIRE

    Llorca Díaz Javier; Prieto Salceda Dolores; Dierssen Sotos Trinidad; Delgado-Rodríguez Miguel

    2000-01-01

    FUNDAMENTO: Analizar el efecto que tiene la elección de una población de referencia sobre diferentes indicadores derivados de las tasas de mortalidad ajustadas por edad. MÉTODOS: Las tasas de mortalidad por diferentes causas de muerte en España de 1971 a 1992 son ajustadas empleando cuatro poblaciones de referencia: poblaciones españolas de 1971 y 1992, población estándar europea y población estándar mundial. Los resultados obtenidos con las cuatro poblaciones se comparan empleando tres indic...

  13. EST analysis in Ginkgo biloba: an assessment of conserved developmental regulators and gymnosperm specific genes

    Directory of Open Access Journals (Sweden)

    Runko Suzan J

    2005-10-01

    Full Text Available Abstract Background Ginkgo biloba L. is the only surviving member of one of the oldest living seed plant groups with medicinal, spiritual and horticultural importance worldwide. As an evolutionary relic, it displays many characters found in the early, extinct seed plants and extant cycads. To establish a molecular base to understand the evolution of seeds and pollen, we created a cDNA library and EST dataset from the reproductive structures of male (microsporangiate, female (megasporangiate, and vegetative organs (leaves of Ginkgo biloba. Results RNA from newly emerged male and female reproductive organs and immature leaves was used to create three distinct cDNA libraries from which 6,434 ESTs were generated. These 6,434 ESTs from Ginkgo biloba were clustered into 3,830 unigenes. A comparison of our Ginkgo unigene set against the fully annotated genomes of rice and Arabidopsis, and all available ESTs in Genbank revealed that 256 Ginkgo unigenes match only genes among the gymnosperms and non-seed plants – many with multiple matches to genes in non-angiosperm plants. Conversely, another group of unigenes in Gingko had highly significant homology to transcription factors in angiosperms involved in development, including MADS box genes as well as post-transcriptional regulators. Several of the conserved developmental genes found in Ginkgo had top BLAST homology to cycad genes. We also note here the presence of ESTs in G. biloba similar to genes that to date have only been found in gymnosperms and an additional 22 Ginkgo genes common only to genes from cycads. Conclusion Our analysis of an EST dataset from G. biloba revealed genes potentially unique to gymnosperms. Many of these genes showed homology to fully sequenced clones from our cycad EST dataset found in common only with gymnosperms. Other Ginkgo ESTs are similar to developmental regulators in higher plants. This work sets the stage for future studies on Ginkgo to better understand seed and

  14. EST analysis in Ginkgo biloba: an assessment of conserved developmental regulators and gymnosperm specific genes

    Science.gov (United States)

    Brenner, Eric D; Katari, Manpreet S; Stevenson, Dennis W; Rudd, Stephen A; Douglas, Andrew W; Moss, Walter N; Twigg, Richard W; Runko, Suzan J; Stellari, Giulia M; McCombie, WR; Coruzzi, Gloria M

    2005-01-01

    Background Ginkgo biloba L. is the only surviving member of one of the oldest living seed plant groups with medicinal, spiritual and horticultural importance worldwide. As an evolutionary relic, it displays many characters found in the early, extinct seed plants and extant cycads. To establish a molecular base to understand the evolution of seeds and pollen, we created a cDNA library and EST dataset from the reproductive structures of male (microsporangiate), female (megasporangiate), and vegetative organs (leaves) of Ginkgo biloba. Results RNA from newly emerged male and female reproductive organs and immature leaves was used to create three distinct cDNA libraries from which 6,434 ESTs were generated. These 6,434 ESTs from Ginkgo biloba were clustered into 3,830 unigenes. A comparison of our Ginkgo unigene set against the fully annotated genomes of rice and Arabidopsis, and all available ESTs in Genbank revealed that 256 Ginkgo unigenes match only genes among the gymnosperms and non-seed plants – many with multiple matches to genes in non-angiosperm plants. Conversely, another group of unigenes in Gingko had highly significant homology to transcription factors in angiosperms involved in development, including MADS box genes as well as post-transcriptional regulators. Several of the conserved developmental genes found in Ginkgo had top BLAST homology to cycad genes. We also note here the presence of ESTs in G. biloba similar to genes that to date have only been found in gymnosperms and an additional 22 Ginkgo genes common only to genes from cycads. Conclusion Our analysis of an EST dataset from G. biloba revealed genes potentially unique to gymnosperms. Many of these genes showed homology to fully sequenced clones from our cycad EST dataset found in common only with gymnosperms. Other Ginkgo ESTs are similar to developmental regulators in higher plants. This work sets the stage for future studies on Ginkgo to better understand seed and pollen evolution, and to

  15. Psicologia Organizacional e do Trabalho: Relato de Experiência em Estágio Supervisionado

    OpenAIRE

    Martins-Silva, Priscilla de Oliveira; Silva Junior, Annor da; Libardi, Mayra Barcelos Amado de Oliveira

    2015-01-01

    Resumo Trata-se de relato de experiência em estágio supervisionado em Psicologia Organizacional e do Trabalho (POT) envolvendo um grupo de alunos de uma Instituição de Educação Superior (IES) localizada no Estado do Espírito Santo. O objetivo central foi discutir os aspectos teóricos e práticos que influenciaram na formação profissional do discente em Psicologia, tendo como foco o estágio supervisionado. Além de relatar a experiência, foi realizado um levantamento junto aos ex-alunos sobre as...

  16. L'entreprise publique est-elle nécessairement moins efficace?

    OpenAIRE

    Gérard Charreaux

    1997-01-01

    L'objectif de cet article est de réexaminer la question de l'inefficience des entreprises publiques à la lumière de la théorie de la gouvernance des entreprises. Deux questions principales sont posées. Quelles sont les différences principales entre les systèmes de gouvernance des entreprises publiques et des entreprises privées? Dans quelle mesure et par quelles voies ces différences de systèmes peuvent-elles expliquer les écarts de performance? La réflexion est notamment articulée autour de ...

  17. Evaluación estética de seis tipos de coronas para dientes primarios

    OpenAIRE

    Héctor Alejandro Ramírez Peña; Eyra Elvyra Rangel Padilla; Héctor R. Martínez-Menchaca; Gerardo Rivera Silva; Gabriela Arredondo Campo; Natalia Barba Borrego; Gustavo Israel Martínez; Roberto Valencia Hitte

    2017-01-01

    Objetivo: Evaluar las preferencias estéticas en relación con el color y la forma de coronas primarias utilizadas para dientes incisivos superiores primarios, mediante la realización de una encuesta a miembros de la Academia Mexicana de Odontología Pediatrica (AMOP). Material y Métodos: Se establecieron seis grupos de estudio con seis coronas diferentes: grupo 1, coronas de zirconia EZ-Pedo; grupo 2, coronas de zirconia NuSmile Zr; grupo 3, coronas estéticas hechas en el consultorio; grupo 4, ...

  18. La Etica Periodística está relegada en Chile

    Directory of Open Access Journals (Sweden)

    Pablo Portales

    2015-01-01

    Full Text Available El realce o el deterioro de la ética periodística en Chile está fuertemente condicionado por el contexto político y económico en que se desenvuelve el periosmo. A continuación describiremos la líneas gruesas del marco jurídico-político y económico en que están insertos los medios de información. Finalmente se mostrarán las características del comportamiento ético.

  19. Notas críticas sobre el aprendizaje estético

    Directory of Open Access Journals (Sweden)

    Pablo García Andreva

    2013-05-01

    Full Text Available El presente trabajo es una reflexión en torno a la estética como uno de los principales factores que vertebran los patrones de la conciencia social. Un acercamiento a las influencias de la plástica, -referente de la actividad creadora que coordina los aspectos emocionales e intelectuales en la educación del niño-, nos mostrará la importancia que conlleva el hecho de mediar en el aprendizaje de un equilibrado juicio estético.

  20. Plan de negocio de un centro de medicina estética

    OpenAIRE

    Moreira Chulilla, Sara

    2014-01-01

    La preparación y la redacción de un plan de negocio puede ser una labor relativamente compleja para personas poco dadas al proceso de reflexión, que es un perfil muy común entre los emprendedores. Sin embargo, y a pesar de estas dificultades, su elaboración es altamente beneficiosa para todo tipo de empresas, tanto de nueva creación como aquellas que ya están en funcionamiento. El plan de negocio que atañe a esta empresa es de una clínica de medicina estética, y se ha decidido hacerlo sobr...

  1. Avaliação dos fatores determinantes da estética do perfil facial

    OpenAIRE

    Reis,Sílvia Augusta Braga; Abrão,Jorge; Claro,Cristiane Aparecida de Assis; Capelozza Filho,Leopoldino

    2011-01-01

    OBJETIVO: avaliar a influência da idade, do sexo, da relação oclusal sagital, do Padrão Facial e de 8 medidas do perfil facial sobre a estética do perfil. MÉTODOS: foram utilizadas tabelas de contingência, o Teste Qui-quadrado e o coeficiente de Cramér para avaliar a possível associação entre a nota dada por 32 avaliadores (14 ortodontistas, 12 leigos e 6 artistas) para a estética do perfil de 100 brasileiros - adultos, leucodermas, portadores de selamento labial passivo - e a idade, o sexo, ...

  2. Texto guía de estática y resistencia de materiales

    OpenAIRE

    De la Cruz Morales, Claudia Jenny

    1996-01-01

    El siguiente trabajo consta de la unión de la estática como asignatura con la resistencia de materiales que se dictan en la carrera de ingeniería civil. En su primera etapa se presentan unas generalidades de la estática en cuando al manejo de vectores, cuerpo rígido, equilibrio de cuerpo rígido, etc. y algunos ejemplos. El tema de elementos prismáticos sometidos a fuerza axial ya inicia un acercamiento a la resistencia de materiales, más directo, en forma genérica se plantea el estado ...

  3. Plataforma de ensayo de inductancias para convertidores estáticos

    OpenAIRE

    Román Lumbreras, Manuel; Velasco Quesada, Guillermo; Conesa Roca, Alfons; Pérez Delgado, Raúl; Codina, Albert; González, Jaime

    2009-01-01

    Reproducido con autorización del Grupo Tecnipublicaciones Este artículo presenta una plataforma para la realización de ensayos, en condiciones reales, sobre las inductancias que se instalarán en serie entre los convertidores estáticos y la red eléctrica en centrales solares fotovoltaicas con un consumo mínimo sin necesidad de convertidores y paneles para la generación fotovoltaica. Su finalidad es servir tanto para el ensayo y el control de la calidad de las inductancias fabric...

  4. Quatro diferentes visões sobre o estágio supervisionado

    OpenAIRE

    Rodrigues,Micaías Andrade

    2013-01-01

    O estágio supervisionado de ensino é um momento muito importante para os estudantes durante os cursos de formação inicial de professores. Buscando compreender melhor esse momento, foi realizada uma pesquisa que objetivou compreender quatro diferentes visões sobre o estágio: a visão legal, a visão dos autores que estudam a temática e a visão dos professores colaboradores. Além de pesquisas bibliográficas e documentais, foram coletadas respostas de 72 alunos de dois cursos de licenciatura da Un...

  5. Estímulos e barreiras à criatividade no ambiente de trabalho

    OpenAIRE

    Faria, Maria de Fátima Bruno de; Alencar, Eunice Maria Lima Soriano de

    1996-01-01

    Por este estudo objetivou-se investigar estímulos e barreiras à criatividade no ambiente das organizações, com a finalidade de subsidiar a futura construção de instrumento que possibilite identificar fatores ambientais que afetam a criatividade, presentes no dia-a-dia das organizações brasileiras. A amostra consistiu de 25 profissionais de distintas organizações do Distrito Federal, 19 do sexo feminino e seis do masculino, 20 sujeitos com formação universitária e cinco com segundo grau. Todos...

  6. Relatório de estágio II de Rita Daniela Cerqueira Ferreira

    OpenAIRE

    Ferreira, Rita Daniela Cerqueira

    2017-01-01

    A unidade curricular de Estágio II, no âmbito do Mestrado Integrado em Ciências Farmacêuticas do Instituto Universitário de Ciências da Saúde, a qual se assume como um complemento aos conhecimentos adquiridos ao longo dos cinco anos. O presente relatório descreve todas as atividades desenvolvidas nos Serviços Farmacêuticos do Centro Hospitalar do Porto (CHP), bem como da Farmácia Cabanelas, ao longo dos cinco meses de estágio, dando a conhecer a organização e o papel do farmacêutico em ca...

  7. Manejo integral estético del sector anterior en el paciente con fluorosis dental

    OpenAIRE

    Giandoménico Villota, Camilo Andrés

    2014-01-01

    Actualmente, la odontología estética ha alcanzado gran importancia en la población general, lo cual se evidencia en la alta demanda de pacientes que buscan una sonrisa armónica, bella, blanca y llamativa. Uno de los principales problemas que más aqueja a la población colombiana y de relevancia en salud pública es la fluorosis dental, que se describe como una “patología endémica asociada al exceso de ingesta del ion flúor, cuya manifestación dental acarrea un problema estético que se caracteri...

  8. Baisse de la transmission de la douve du foie dans le nord-est de la ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    mortelle de cancer du foie liée à l'O. viverrini. Dans cer- taines régions, près de 85 % de la population est porteuse du parasite. Les villages du nord-est de la Thaïlande, où l'on raffole du poisson cru, ont des taux d'infection parmi les plus élevés du pays. Les personnes infectées peuvent porter le parasite adulte pendant ...

  9. Relatório de estágio na empresa A Equipa

    OpenAIRE

    Ávila, Marta Pena da Costa

    2017-01-01

    O presente relatório pretende descrever a experiência de estágio da estudante Marta Pena da Costa de Ávila na agência criativa A EQUIPA. A realização do estágio teve como objetivo principal tomar contacto com a realidade profissional a partir da área do design, possibilitar a implementação de conhecimentos adquiridos durante o mestrado, ampliando a sua compreensão e desenvolvendo competências em contexto laboral. ...

  10. Conservación de la cepa Lecanicillium lecanii en agua destilada estéril

    OpenAIRE

    Angela Ofelia Díaz Llanes; Teresita Lemes Rodríguez

    2009-01-01

    Se evaluó el método de preservación en agua destilada para el hongo entomopatógeno Lecanicillium lecanii, con el objetivo de definir sus posibilidades de aplicación en la conservación de este hongo. El estudio se realizó inoculando asépticamente las esporas en bulbos que contenían agua destilada estéril y fueron almacenados a temperatura ambiente y refrigeración. La efectividad del método fue evaluada mediante determinaciones de viabilidad, características macroscópicas y microscópicas del ho...

  11. Relatório de Estágio Curricular – Adecco (Viseu)

    OpenAIRE

    Almeida, Ana

    2012-01-01

    O trabalho desenvolvido ao longo de dois meses e meio incidiu na área dos Recursos Humanos, sobretudo no trabalho temporário, na empresa Adecco. No decorrer do estágio tive a oportunidade de observar e participar nas diversas tarefas que foram surgindo. Todas estas oportunidades tiveram como finalidade a aquisição de um conjunto de competências que permitiram que o estágio fosse bastante enriquecedor. Esta aprendizagem focou-se nas áreas do recrutamento e seleção, onde efetuava o atendi...

  12. El origen de la cerámica en el Este de Asia

    OpenAIRE

    Sola Fernández de Zuazo, Garazi

    2017-01-01

    Este trabajo trata sobre el origen de la cerámica. Las teorías tradicionales situaban esta innovación en el Próximo Oriente durante la “Revolución Neolítica” descrita por Gordon Childe. En cambio, los nuevos descubrimientos realizados en yacimientos del Este de Asia nos muestran que la cerámica apareció varios milenios antes en esta zona. La cerámica más antigua fue hallada en yacimientos en cuevas del sur de China y presenta una cronología varios milenios anterior a la aparición de asentamie...

  13. El cuidado de enfermeria ante los procesos quirúrgicos estéticos

    OpenAIRE

    Laguado Jaimes, Elveny; Yaruro Bacca, Karol; Hernández Calderón, Emily Jurani

    2015-01-01

    Introducción: El cuidado de enfermería dado en diferentes situaciones no puede estar ajeno al auge de las cirugías estéticas y su relación con la construcción social y cultural del significado del cuerpo, y las respuestas humanas de las personas que deben ser intervenidas. Desde algunas perspectivas sociológicas se describe la influencia de los paradigmas estéticos sobre los ideales y las prácticas de transformación actuales que se realizan los individuos para alcanzar la belleza ...

  14. Teatros negros e suas estéticas na cena teatral brasileira

    OpenAIRE

    Jesus, Cristiane Sobral Correa

    2016-01-01

    Este trabalho procurará refletir sobre a trajetória de alguns artistas independentes e grupos de teatro negro brasileiro que atuaram antes e depois do Teatro Experimental do Negro, companhia teatral criada nos anos quarenta por Abdias do Nascimento, mapeando suas especificidades, singularidades estéticas, conquistas, avanços e desafios impostos pela realidade do cenário cultural brasileiro. E por sabermos que os artistas negros de ontem e de hoje ainda são caracterizados a partir de estereóti...

  15. Isolation and Characterization of Novel EST-Derived Genic Markers in Pisum sativum (Fabaceae

    Directory of Open Access Journals (Sweden)

    Shalu Jain

    2013-10-01

    Full Text Available Premise of the study: Novel markers were developed for pea (Pisum sativum from pea expressed sequence tags (ESTs having significant homology to Medicago truncatula gene sequences to investigate genetic diversity, linkage mapping, and cross-species transferability. Methods and Results: Seventy-seven EST-derived genic markers were developed through comparative mapping between M. truncatula and P. sativum in which 75 markers produced PCR products and 33 were polymorphic among 16 pea genotypes. Conclusions: The novel markers described here will be useful for future genetic studies of P. sativum; their amplification in lentil (Lens culinaris demonstrates their potential for use in closely related species.

  16. El Auditorio Inquietante: Estética sonora en los artgames

    OpenAIRE

    Camacho Blanco, Luis Felipe

    2017-01-01

    Los videojuegos se empiezan a erigir como el género artístico predominante del siglo XXI. Con un lenguaje ya maduro y una estética propia, aquellas limitaciones que tenían otrora se han convertido en grandes herramientas estéticas y semióticas con las que desencadenar distintos procesos emocionales. El sonido y la música han conseguido tener sus propias funciones dentro de los artgames, diferenciadas de las de otros medios audiovisuales, y lo más importante, se ha creado una so...

  17. Gestión de proyectos digitales bajo el estándar PMBOK®

    OpenAIRE

    García Vas, Ricardo

    2016-01-01

    The objective of this final degree project is introduce the PMBOK® standard for the management of a project in a communication and technology company. El siguiente proyecto final de carrera tiene como objetivo la implementación del estándar PMBOK® para la gestión de un proyecto en una empresa de comunicación y tecnología. El següent projecte final de carrera té com a objectiu la implementació de l'estàndard PMBOK® per a la gestió d'un projecte en una empresa de comunicació i tecnologia....

  18. Relatório de Estágio: Clube Desportivo Candal

    OpenAIRE

    Menezes, Guilherme Corrêa

    2013-01-01

    O presente relatório foi elaborado na sequência do estágio desenvolvido no âmbito do curso de Mestrado em Fisioterapia, opção Desporto da Escola Superior de Tecnologia da Saúde do Porto, com o propósito de obtenção do grau de Mestre. O estágio foi realizado no Clube Desportivo Candal em Vila Nova de Gaia, entre os meses de setembro e dezembro de 2012, num total de aproximadamente 200 horas, tendo como o principal objetivo aprofundar/desenvolver competências específicas da prática profissio...

  19. Analysis of gene expression by ESTs from suppression subtractive hybridization library in Chenopodium album L. under salt stress.

    Science.gov (United States)

    Gu, Lili; Xu, Dongsheng; You, Tianyu; Li, Xiuming; Yao, Shixiang; Chen, Shasha; Zhao, Juan; Lan, Haiyan; Zhang, Fuchun

    2011-11-01

    To identify genes expression in Chenopodium album exposed to NaCl stress and screen ESTs related to salt stress, a subtractive suppression hybridization (SSH) library of C. album under salt stress was constructed in the present study. Random EST sequencing produced 825 high-quality ESTs with GenBank ID GE746311-GE747007, which had 301 bp of average size and were clustered into 88 contigs and 550 singletons. They were classified into 12 categories according to their function annotations. 635 ESTs (76.97%) showed similarities to gene sequences in the non-redundancy database, while 190 ESTs (23.03%) showed low or no similarities. The transcriptional profiles of 56 ESTs randomly selected from 347 unknown or novel ESTs of SSH library under varying NaCl concentration and at different time points were analyzed. The results indicated that a high proportion of tested ESTs were activated by salt stress. Four in 56 ESTs responded to NaCl were also enhanced in expression level when exposed to ABA and PEG stresses. The above four ESTs were validated by northern blotting which was consistent with the results of RT-PCR. The results suggested that genes corresponded to these ESTs might be involved in stress response or regulation. The complete sequences and detailed function of these ESTs need to be further studied.

  20. "Need, kes on käest libisenud..." : [luuletused] / Kalev Kesküla

    Index Scriptorium Estoniae

    Kesküla, Kalev, 1959-2010

    2000-01-01

    Sisu: "Need, kes on käest libisenud..." ; Armastuse kolmjalg ; Inimese määratlus ; Aja määratlus ; Taasleitud Pariis ; Dr. Faustus ; "Päev, mu tegus tööandja..." ; "Mõned kadunud asjad..." ; "Keskealine mees..." ; "Unustuse keha..."

  1. Generation of EST and Microarray Resources for Functional Genomic Studies on Chicken Intestinal Health

    NARCIS (Netherlands)

    Hemert, van S.; Ebbelaar, B.H.; Smits, M.A.; Rebel, J.M.J.

    2003-01-01

    Expressed sequenced tags (ESTs) and microarray resources have a great impact on the ability to study host response in mice and humans. Unfortunately, these resources are not yet available for domestic farm animals. The aim of this study was to provide genomic resources to study chicken intestinal

  2. Imágenes mentales I. Los estímulos visuales y auditivos

    Directory of Open Access Journals (Sweden)

    Isidoro Arroyo Almaraz

    2012-04-01

    Full Text Available Se investigan las características del recuerdo de las imágenes mentalesgeneradas por estímulos, según: a modalidad sensorial de presentación:visual o auditiva (palabra y sonido, b grado de concreción, c grado deriqueza de la imagen mental (I.M..Los resultados obtenidos demuestran que la capacidad de recuerdo de lasimágenes mentales depende de la modalidad de presentación del estímulo y de su grado de concreción. Si la modalidad es icónica, la imagen mental será mejor recordada, cuanto más rico sea el estímulo en rasgos figurativos. Si la modalidad es auditiva, la capacidad de recuerdo de la imagen mental, dependerá, por una parte, de la capacidad del estímulo parapermitir la subvocalización. Y, por otra parte, de la capacidad individualpara crear una representación visual de un sonido.

  3. Cluster - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ...e File name: clest_cluster.zip File URL: ftp://ftp.biosciencedbc.jp/archive/clest/LATEST/clest_cluster.zip F...ownload License Update History of This Database Site Policy | Contact Us Cluster - ClEST | LSDB Archive ...

  4. Clone - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data ...le URL: ftp://ftp.biosciencedbc.jp/archive/clest/LATEST/clest_clone.zip File size: 660 KB Simple search URL ...ion Download License Update History of This Database Site Policy | Contact Us Clone - ClEST | LSDB Archive ...

  5. Development of new candidate gene and EST-based molecular markers for Gossypium species

    Science.gov (United States)

    New source of molecular markers accelerates the efforts in improving cotton fiber traits and aid in developing high-density integrated genetic maps. We developed new markers based on candidate genes and G. arboreum expressed sequence tag (EST) sequences, and validated them through amplification, ge...

  6. Genome-wide analysis of immune system genes by EST profiling

    Science.gov (United States)

    Giallourakis, Cosmas; Benita, Yair; Molinie, Benoit; Cao, Zhifang; Despo, Orion; Pratt, Henry E.; Zukerberg, Lawrence R.; Daly, Mark J.; Rioux, John D.; Xavier, Ramnik J.

    2013-01-01

    Profiling studies of mRNA and miRNA, particularly microarray-based studies, have been extensively used to create compendia of genes that are preferentially expressed in the immune system. In some instances, functional studies have been subsequently pursued. Recent efforts such as ENCODE have demonstrated the benefit of coupling RNA-Seq analysis with information from expressed sequence tags (ESTs) for transcriptomic analysis. However, the full characterization and identification of transcripts that function as modulators of human immune responses remains incomplete. In this study, we demonstrate that an integrated analysis of human ESTs provides a robust platform to identify the immune transcriptome. Beyond recovering a reference set of immune-enriched genes and providing large-scale cross-validation of previous microarray studies, we discovered hundreds of novel genes preferentially expressed in the immune system, including non-coding RNAs. As a result, we have established the Immunogene database, representing an integrated EST “road map” of gene expression in human immune cells, which can be used to further investigate the function of coding and non-coding genes in the immune system. Using this approach, we have uncovered a unique metabolic gene signature of human macrophages and identified PRDM15 as a novel overexpressed gene in human lymphomas. Thus we demonstrate the utility of EST profiling as a basis for further deconstruction of physiologic and pathologic immune processes. PMID:23616578

  7. Suur vigurmarssimine EST-TATTOO 2004 / Kerstin Käärik

    Index Scriptorium Estoniae

    Käärik, Kerstin

    2004-01-01

    12. novembril toimus Saku Suurhallis vigurmarsietendus EST-TATTOO 2004, üritusega tähistati Eesti ühinemist NATO-ga. Esinesid Läti ja Leedu piirivalveorkestrid, Eesti Kaitseväe Orkester, Piirivalve Orkester ning Politseiorkester. Üritusel osales ka president Arnold Rüütel

  8. , TOUCHE PAS À MA TERRE, I ! ' C'EST MA VIE !

    International Development Research Centre (IDRC) Digital Library (Canada)

    .interpares.ca. Fondée en 1975, Inter Pares, dont le nom signifie « entre égaux », est une organisation canadienne de justice sociale œuvrant au Canada et dans le Sud globalisé. Inter Pares s'attache à faire connaître les causes et les effets ...

  9. Heidegger: Poesía, estética y verdad

    Directory of Open Access Journals (Sweden)

    Marta null

    2010-01-01

    Full Text Available El análisis de la concepción del lenguaje en Heidegger nos sirve como punto de partida e hilo conductor para explicar su teoría estética y la vinculación y relaciones entre estética y ontología; y entre estética y verdad, entendida ésta como aletheia, como un "sacar del olvido", como "des-ocultación". En segundo lugar, analizamos las características constitutivas del Da-sein, en cuanto ser-en-el-mundo, desde el punto de vista del lenguaje. En tercer lugar, al considerar el horizonte histórico como ontológicamente constitutivo del Da-sein, intentamos explicar qué significa esta temporalidad desde la vida auténtica y qué significa crear; y también, por qué la creación de una obra de arte ocupa un lugar preferencial en relación con la propuesta heideggeriana. Nos preguntamos, por último, qué es Poesía para Heidegger y en qué sentidos es indisociable de la verdad (aletheia. E intentamos interpretar el alcance de la Poesía (Dichtung en relación con la estética, así como del lenguaje en relación con la verdad.

  10. Captura da atenção por estímulos emocionais

    Directory of Open Access Journals (Sweden)

    Fátima Erthal

    2004-04-01

    Full Text Available No presente estudo investigamos se estímulos emocionais distrativos são capazes de interferir na realização de tarefas nas quais é exigido um alto engajamento da atenção. Para tanto foi realizado um teste de discriminação de orientação entre duas barras apresentadas bilateralmente na periferia do campo visual (julgamento igual ou diferente. O grau de dificuldade da tarefa dependia da diferença de orientação das mesmas. Simultaneamente era apresentada uma figura central (neutra ou negativa entre as barras. Foi observado que as figuras negativas interferiram no desempenho dos voluntários mesmo na tarefa mais difícil, que exigia maior engajamento atencional. Além disto, quando o reconhecimento posterior destas figuras era testado, figuras negativas eram mais lembradas do que neutras. Estes dados sugerem que estímulos emocionais são privilegiados na disputa por recursos de atenção. É possível que esse privilégio ocorra devido a relevância destes estímulos, sendo dificilmente ignorados.

  11. Identification de Staphylococcus aureus : est-il possible de réduire ...

    African Journals Online (AJOL)

    SARAH

    31 janv. 2014 ... influencer la recherche de la staphylocoagulase libre ? 6027. Identification de Staphylococcus aureus : est-il possible de réduire le volume de plasma de lapin sans influencer la recherche de la staphylocoagulase libre ? Bankolé H.S., Dougnon T.V., Fiogbé E.P., Amoussou A.N., Baba-Moussa L.

  12. Estéticas y Políticas del Nazismo

    Directory of Open Access Journals (Sweden)

    Jordi Claramonte Arrufat

    2013-07-01

    Full Text Available Cuando se alude a las Estéticas características del Nazismo, solemos empezar por pensar en grandes desfiles, encuentros de la juventud, marchas de antorchas, masivos sermones al aire libre y multitudes enardecidas dispuestas a lanzarse a degüello sobre el enemigo más próximo. Sin embargo no es eso lo que se desprende en absoluto, ni de un análisis de los materiales y prácticas, ni del estudio de los textos y declaraciones de los principales responsables de la producción estética del nazismo. Veremos en este artículo, por el contrario, que la apuesta de la inmensa mayoría de la producción estética nazi estuvo vinculada a la producción y estabilización de ciudadanos tan normales y razonables como nos sea dado concebir hoy mismo.

  13. In silico comparative analysis of EST-SSRs in three cotton genomes ...

    African Journals Online (AJOL)

    The frequency of EST-SSRs was highest in Gr, and motif type for hexanucleotide was obviously abundant in Gr. Trinucleotide repeats were the most abundant motif; AT and AG, AAG and ATC were the most frequent motifs for dinucleotide and trinucleotide, respectively. The repeat number was greatly diverse between the ...

  14. Development and Testing of New Gene-Homologous EST-SSRs for Eucalyptus gomphocephala (Myrtaceae

    Directory of Open Access Journals (Sweden)

    Donna Bradbury

    2013-07-01

    Full Text Available Premise of the study: New microsatellite (simple sequence repeat [SSR] primers were developed from Eucalyptus expressed sequence tags (ESTs and optimized for genetic studies of the southwestern Australian tree E. gomphocephala, which is severely impacted by tree health decline and habitat fragmentation. Methods and Results: A total of 133 gene-homologous EST-SSR primer pairs were designed for Eucalyptus, and 44 were screened in E. gomphocephala. Of these, 17 produced reliable amplification products and 11 were polymorphic. Between two and 13 alleles were observed per locus, and observed heterozygosities ranged from 0.172 to 0.867. All 17 EST-SSRs that amplified E. gomphocephala cross-amplified to at least one of E. marginata, E. camaldulensis, and E. victrix. Conclusions: This set of EST-SSR primer pairs will be valuable tools for future population genetic studies of E. gomphocephala and other eucalypts, particularly for studying gene-linked variation and informing seed-sourcing strategies for ecological restoration.

  15. Análisis estático de seguridad en redes de transporte

    OpenAIRE

    Riquelme Santos, Jesús Manuel

    1998-01-01

    La creciente complejidad de la red eléctrica hace que el estudio de la seguridad sea un problema costoso computacionalmente. Por tanto se plantea la necesidad de disponer de una herramienta adecuada que facilite este análisis. Se inició pues, en colaborac

  16. Extra heavy oil and refinery residues upgrading through Eni Slurry Technology : first EST commercial unit

    Energy Technology Data Exchange (ETDEWEB)

    Rispoli, G.; Sanfilippo, D.; Amoroso, A [Eni S.p.A., Rome (Italy)

    2009-07-01

    The production of heavy crude oils is projected to continue to grow in the upstream oil industry given that large reserves of unconventional extra heavy crude and bitumen exist in several geographic areas including Canada and Venezuela. As reserves of conventional crude oil continue to decline, these unconventional feedstocks are becoming an opportunity to pursue, but they require effective technologies for upgrading and meeting the growing demand for light and middle distillate fuels. This paper described the proprietary technology that offers a solution to upstream and downstream oil producers for bottom-of-the-barrel upgrading. En i Slurry Technology (EST) is constructing an industrial plant in its Sannazzaro refinery in Italy. The plant is designed to convert 23,000 BPSD of vacuum residue into high quality diesel and other valuable refinery streams such as liquefied petroleum gas, naphtha and jet fuel. EST is an H-addition process characterized by the use of a special homogeneous isothermal intrinsically safe reactor, and of a nano-dispersed non-ageing catalyst. EST converts more than 98 per cent of any type of residues to about 110 per cent volume of light products and distillates or extra heavy oils to high quality bottomless SCO. In typical performance, HDS is greater than 85 per cent, HDM greater than 99 per cent and HDCCR greater than 97 per cent. EST also achieves the target of zero fuel oil - zero coke. 12 refs., 4 tabs., 5 figs.

  17. SSR mining in oil palm EST database: application in oil palm ...

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Genetics; Volume 89; Issue 2. SSR mining in oil palm EST ... Research Article Volume 89 Issue 2 August 2010 pp 135-145 ... The polymerase chain reaction (PCR) products of three primer-pairs detected in E. guineensis, E. oleifera, C. nucifera and Jessinia bataua were cloned and sequenced.

  18. Faire campagne en ville : L'agriculture urbaine en Afrique de l'Est ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Faire campagne en ville : L'agriculture urbaine en Afrique de l'Est. Couverture du livre Faire campagne en ville : L'agriculture urbaine en Afrique de l. Auteur(s):. Auxumite G. Egziabher, Diana Lee-Smith, Daniel G. Maxwell, Pyar Ali Memon, Luc Mougeot et Camillus J. Sawio. Maison(s) d'édition: CRDI. 1 janvier 1995. ISBN :.

  19. 1 1) Pourquoi est-ce que je ne parviens pas à ouvrir les formulaires ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    IDRC CRDI

    Faites vos calculs avec soin car votre demande pourrait ne pas être admissible.) 6) Existe-t-il une liste de pays admissibles dans lesquels mon établissement ou organisme est autorisé à travailler ou à avoir des partenaires ? Non, le CRDI n'a rien de tel; cependant, il ne soutient des travaux que dans les pays à faible.

  20. Estética urbana: uma análise através das ideias de ordem, estímulo visual, valor histórico e familiaridade

    Directory of Open Access Journals (Sweden)

    Antônio Tarcísio da Luz Reis

    Full Text Available Este artigo examina a dicotomia entre a abordagem da estética filosófica e a da estética empírica, assim como os impactos da estética formal e da estética simbólica, a partir das respostas de usuários do espaço urbano de Porto Alegre. São investigados os impactos estéticos causados por cenas urbanas com diferentes níveis de ordem e estímulo visual, com e sem valor histórico, e com diferentes níveis de familiaridade. É também examinada a existência ou não de diferenças entre as respostas estéticas de arquitetos, não arquitetos com curso superior e pessoas com o primeiro ou segundo grau. Ainda, são identificadas as razões para as avaliações realizadas por esses três grupos. Os principais resultados evidenciam o potencial da estética empírica em explicar as avaliações estéticas e revelam o impacto positivo e preponderante da ideia de ordem e estímulo visual em tais avaliações.

  1. Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas).

    Science.gov (United States)

    Wang, Zhangying; Li, Jun; Luo, Zhongxia; Huang, Lifei; Chen, Xinliang; Fang, Boping; Li, Yujun; Chen, Jingyi; Zhang, Xiongjian

    2011-10-20

    Currently there exists a limited availability of genetic marker resources in sweetpotato (Ipomoea batatas), which is hindering genetic research in this species. It is necessary to develop more molecular markers for potential use in sweetpotato genetic research. With the newly developed next generation sequencing technology, large amount of transcribed sequences of sweetpotato have been generated and are available for identifying SSR markers by data mining. In this study, we investigated 181,615 ESTs for the identification and development of SSR markers. In total, 8,294 SSRs were identified from 7,163 SSR-containing unique ESTs. On an average, one SSR was found per 7.1 kb of EST sequence with tri-nucleotide motifs (42.9%) being the most abundant followed by di- (41.2%), tetra- (9.2%), penta- (3.7%) and hexa-nucleotide (3.1%) repeat types. The top five motifs included AG/CT (26.9%), AAG/CTT (13.5%), AT/TA (10.6%), CCG/CGG (5.8%) and AAT/ATT (4.5%). After removing possible duplicate of published EST-SSRs of sweetpotato, a total of non-repeat 7,958 SSR motifs were identified. Based on these SSR-containing sequences, 1,060 pairs of high-quality SSR primers were designed and used for validation of the amplification and assessment of the polymorphism between two parents of one mapping population (E Shu 3 Hao and Guang 2k-30) and eight accessions of cultivated sweetpotatoes. The results showed that 816 primer pairs could yield reproducible and strong amplification products, of which 195 (23.9%) and 342 (41.9%) primer pairs exhibited polymorphism between E Shu 3 Hao and Guang 2k-30 and among the 8 cultivated sweetpotatoes, respectively. This study gives an insight into the frequency, type and distribution of sweetpotato EST-SSRs and demonstrates successful development of EST-SSR markers in cultivated sweetpotato. These EST-SSR markers could enrich the current resource of molecular markers for the sweetpotato community and would be useful for qualitative and quantitative

  2. A set of EST-SNPs for map saturation and cultivar identification in melon

    Directory of Open Access Journals (Sweden)

    Monforte Antonio J

    2009-07-01

    Full Text Available Abstract Background There are few genomic tools available in melon (Cucumis melo L., a member of the Cucurbitaceae, despite its importance as a crop. Among these tools, genetic maps have been constructed mainly using marker types such as simple sequence repeats (SSR, restriction fragment length polymorphisms (RFLP and amplified fragment length polymorphisms (AFLP in different mapping populations. There is a growing need for saturating the genetic map with single nucleotide polymorphisms (SNP, more amenable for high throughput analysis, especially if these markers are located in gene coding regions, to provide functional markers. Expressed sequence tags (ESTs from melon are available in public databases, and resequencing ESTs or validating SNPs detected in silico are excellent ways to discover SNPs. Results EST-based SNPs were discovered after resequencing ESTs between the parental lines of the PI 161375 (SC × 'Piel de sapo' (PS genetic map or using in silico SNP information from EST databases. In total 200 EST-based SNPs were mapped in the melon genetic map using a bin-mapping strategy, increasing the map density to 2.35 cM/marker. A subset of 45 SNPs was used to study variation in a panel of 48 melon accessions covering a wide range of the genetic diversity of the species. SNP analysis correctly reflected the genetic relationships compared with other marker systems, being able to distinguish all the accessions and cultivars. Conclusion This is the first example of a genetic map in a cucurbit species that includes a major set of SNP markers discovered using ESTs. The PI 161375 × 'Piel de sapo' melon genetic map has around 700 markers, of which more than 500 are gene-based markers (SNP, RFLP and SSR. This genetic map will be a central tool for the construction of the melon physical map, the step prior to sequencing the complete genome. Using the set of SNP markers, it was possible to define the genetic relationships within a collection of forty

  3. Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas

    Directory of Open Access Journals (Sweden)

    Li Yujun

    2011-10-01

    Full Text Available Abstract Background Currently there exists a limited availability of genetic marker resources in sweetpotato (Ipomoea batatas, which is hindering genetic research in this species. It is necessary to develop more molecular markers for potential use in sweetpotato genetic research. With the newly developed next generation sequencing technology, large amount of transcribed sequences of sweetpotato have been generated and are available for identifying SSR markers by data mining. Results In this study, we investigated 181,615 ESTs for the identification and development of SSR markers. In total, 8,294 SSRs were identified from 7,163 SSR-containing unique ESTs. On an average, one SSR was found per 7.1 kb of EST sequence with tri-nucleotide motifs (42.9% being the most abundant followed by di- (41.2%, tetra- (9.2%, penta- (3.7% and hexa-nucleotide (3.1% repeat types. The top five motifs included AG/CT (26.9%, AAG/CTT (13.5%, AT/TA (10.6%, CCG/CGG (5.8% and AAT/ATT (4.5%. After removing possible duplicate of published EST-SSRs of sweetpotato, a total of non-repeat 7,958 SSR motifs were identified. Based on these SSR-containing sequences, 1,060 pairs of high-quality SSR primers were designed and used for validation of the amplification and assessment of the polymorphism between two parents of one mapping population (E Shu 3 Hao and Guang 2k-30 and eight accessions of cultivated sweetpotatoes. The results showed that 816 primer pairs could yield reproducible and strong amplification products, of which 195 (23.9% and 342 (41.9% primer pairs exhibited polymorphism between E Shu 3 Hao and Guang 2k-30 and among the 8 cultivated sweetpotatoes, respectively. Conclusion This study gives an insight into the frequency, type and distribution of sweetpotato EST-SSRs and demonstrates successful development of EST-SSR markers in cultivated sweetpotato. These EST-SSR markers could enrich the current resource of molecular markers for the sweetpotato community and would

  4. Development of ESTs from chickpea roots and their use in diversity analysis of the Cicer genus

    Directory of Open Access Journals (Sweden)

    Eshwar K

    2005-08-01

    Full Text Available Abstract Background Chickpea is a major crop in many drier regions of the world where it is an important protein-rich food and an increasingly valuable traded commodity. The wild annual Cicer species are known to possess unique sources of resistance to pests and diseases, and tolerance to environmental stresses. However, there has been limited utilization of these wild species by chickpea breeding programs due to interspecific crossing barriers and deleterious linkage drag. Molecular genetic diversity analysis may help predict which accessions are most likely to produce fertile progeny when crossed with chickpea cultivars. While, trait-markers may provide an effective tool for breaking linkage drag. Although SSR markers are the assay of choice for marker-assisted selection of specific traits in conventional breeding populations, they may not provide reliable estimates of interspecific diversity, and may lose selective power in backcross programs based on interspecific introgressions. Thus, we have pursued the development of gene-based markers to resolve these problems and to provide candidate gene markers for QTL mapping of important agronomic traits. Results An EST library was constructed after subtractive suppressive hybridization (SSH of root tissue from two very closely related chickpea genotypes (Cicer arietinum. A total of 106 EST-based markers were designed from 477 sequences with functional annotations and these were tested on C. arietinum. Forty-four EST markers were polymorphic when screened across nine Cicer species (including the cultigen. Parsimony and PCoA analysis of the resultant EST-marker dataset indicated that most accessions cluster in accordance with the previously defined classification of primary (C. arietinum, C. echinospermum and C. reticulatum, secondary (C. pinnatifidum, C. bijugum and C. judaicum, and tertiary (C. yamashitae, C. chrossanicum and C. cuneatum gene-pools. A large proportion of EST alleles (45% were only

  5. Characterization and development of EST-derived SSR markers in cultivated sweetpotato (Ipomoea batatas)

    Science.gov (United States)

    2011-01-01

    Background Currently there exists a limited availability of genetic marker resources in sweetpotato (Ipomoea batatas), which is hindering genetic research in this species. It is necessary to develop more molecular markers for potential use in sweetpotato genetic research. With the newly developed next generation sequencing technology, large amount of transcribed sequences of sweetpotato have been generated and are available for identifying SSR markers by data mining. Results In this study, we investigated 181,615 ESTs for the identification and development of SSR markers. In total, 8,294 SSRs were identified from 7,163 SSR-containing unique ESTs. On an average, one SSR was found per 7.1 kb of EST sequence with tri-nucleotide motifs (42.9%) being the most abundant followed by di- (41.2%), tetra- (9.2%), penta- (3.7%) and hexa-nucleotide (3.1%) repeat types. The top five motifs included AG/CT (26.9%), AAG/CTT (13.5%), AT/TA (10.6%), CCG/CGG (5.8%) and AAT/ATT (4.5%). After removing possible duplicate of published EST-SSRs of sweetpotato, a total of non-repeat 7,958 SSR motifs were identified. Based on these SSR-containing sequences, 1,060 pairs of high-quality SSR primers were designed and used for validation of the amplification and assessment of the polymorphism between two parents of one mapping population (E Shu 3 Hao and Guang 2k-30) and eight accessions of cultivated sweetpotatoes. The results showed that 816 primer pairs could yield reproducible and strong amplification products, of which 195 (23.9%) and 342 (41.9%) primer pairs exhibited polymorphism between E Shu 3 Hao and Guang 2k-30 and among the 8 cultivated sweetpotatoes, respectively. Conclusion This study gives an insight into the frequency, type and distribution of sweetpotato EST-SSRs and demonstrates successful development of EST-SSR markers in cultivated sweetpotato. These EST-SSR markers could enrich the current resource of molecular markers for the sweetpotato community and would be useful for

  6. EVER-EST: European Virtual Environment for Research in Earth Science Themes

    Science.gov (United States)

    Glaves, H.; Albani, M.

    2016-12-01

    EVER-EST is an EC Horizon 2020 project having the goal to develop a Virtual Research Environment (VRE) providing a state-of-the-art solution to allow Earth Scientists to preserve their work and publications for reference and future reuse, and to share with others. The availability of such a solution, based on an innovative concept and state of art technology infrastructure, will considerably enhance the quality of how Earth Scientists work together within their own institution and also across other organizations, regions and countries. The concept of Research Objects (ROs), used in the Earth Sciences for the first time, will form the backbone of the EVER-EST VRE infrastructure. ROs will enhance the ability to preserve, re-use and share entire or individual parts of scientific workflows and all the resources related to a specific scientific investigation. These ROs will also potentially be used as part of the scholarly publication process. EVER-EST is building on technologies developed during almost 15 years of research on Earth Science data management infrastructures. The EVER-EST VRE Service Oriented Architecture is being meticulously designed to accommodate at best the requirements of a wide range of Earth Science communities and use cases: focus is put on common requirements and on minimising the level of complexity in the EVER-EST VRE to ensure future sustainability within the user communities beyond the end of the project. The EVER-EST VRE will be validated through its customisation and deployment by four Virtual Research Communities (VRCs) from different Earth Science disciplines and will support enhanced interaction between data providers and scientists in the Earth Science domain. User community will range from bio-marine researchers (Sea Monitoring use case), to common foreign and security policy institutions and stakeholders (Land Monitoring for Security use case), natural hazards forecasting systems (Natural Hazards use case), and disaster and risk

  7. A set of EST-SNPs for map saturation and cultivar identification in melon.

    Science.gov (United States)

    Deleu, Wim; Esteras, Cristina; Roig, Cristina; González-To, Mireia; Fernández-Silva, Iria; Gonzalez-Ibeas, Daniel; Blanca, José; Aranda, Miguel A; Arús, Pere; Nuez, Fernando; Monforte, Antonio J; Picó, Maria Belén; Garcia-Mas, Jordi

    2009-07-15

    There are few genomic tools available in melon (Cucumis melo L.), a member of the Cucurbitaceae, despite its importance as a crop. Among these tools, genetic maps have been constructed mainly using marker types such as simple sequence repeats (SSR), restriction fragment length polymorphisms (RFLP) and amplified fragment length polymorphisms (AFLP) in different mapping populations. There is a growing need for saturating the genetic map with single nucleotide polymorphisms (SNP), more amenable for high throughput analysis, especially if these markers are located in gene coding regions, to provide functional markers. Expressed sequence tags (ESTs) from melon are available in public databases, and resequencing ESTs or validating SNPs detected in silico are excellent ways to discover SNPs. EST-based SNPs were discovered after resequencing ESTs between the parental lines of the PI 161375 (SC) x 'Piel de sapo' (PS) genetic map or using in silico SNP information from EST databases. In total 200 EST-based SNPs were mapped in the melon genetic map using a bin-mapping strategy, increasing the map density to 2.35 cM/marker. A subset of 45 SNPs was used to study variation in a panel of 48 melon accessions covering a wide range of the genetic diversity of the species. SNP analysis correctly reflected the genetic relationships compared with other marker systems, being able to distinguish all the accessions and cultivars. This is the first example of a genetic map in a cucurbit species that includes a major set of SNP markers discovered using ESTs. The PI 161375 x 'Piel de sapo' melon genetic map has around 700 markers, of which more than 500 are gene-based markers (SNP, RFLP and SSR). This genetic map will be a central tool for the construction of the melon physical map, the step prior to sequencing the complete genome. Using the set of SNP markers, it was possible to define the genetic relationships within a collection of forty-eight melon accessions as efficiently as with SSR

  8. Competência Informacional: um olhar para a Dimensão Estética

    Directory of Open Access Journals (Sweden)

    Eliane Rodrigues Mota Orelo

    Full Text Available A Competência Informacional é uma temática que vem se destacando nos últimos anos, dada a sua relevância para a sociedade. Ser competente em informação significa desenvolver um conjunto de habilidades para o uso eficiente dos recursos informacionais, o aprendizado ao longo da vida e o pensamento crítico. Para ser desenvolvida em plenitude, a Competência Informacional precisa fundar-se em quatro dimensões: Técnica, Estética, Ética e Política, mantendo-se o equilíbrio entre elas. Dá ênfase à Dimensão Estética da Competência Informacional: tema ainda obscuro no que se refere ao seu estudo e aplicação na Ciência da Informação. Essa dimensão é aquela que se relaciona com a sensibilidade, com a criatividade. Sendo assim, relacionar a Estética à Competência Informacional se reflete na construção de uma sociedade equilibrada, generosa e transformadora, pois as ideias estéticas são, em última análise, ideias de liberdade humana, estando aí inclusas as emoções, por meio da imaginação, o que possibilita às pessoas perceber propriedades, como encanto, equilíbrio, harmonia e ritmo. A Dimensão Estética relaciona-se, deste modo, com a construção do conhecimento pela sensibilidade, pelas percepções de mundo e sua relação com a ética, pois contribui de forma significativa para o desenvolvimento dos indivíduos.

  9. Generation, annotation, and analysis of ESTs from midgut tissue of adult female Anopheles stephensi mosquitoes

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    Bhagwat Bhakti

    2009-08-01

    Full Text Available Abstract Background Malaria is a tropical disease caused by protozoan parasite, Plasmodium, which is transmitted to humans by various species of female anopheline mosquitoes. Anopheles stephensi is one such major malaria vector in urban parts of the Indian subcontinent. Unlike Anopheles gambiae, an African malaria vector, transcriptome of A. stephensi midgut tissue is less explored. We have therefore carried out generation, annotation, and analysis of expressed sequence tags from sugar-fed and Plasmodium yoelii infected blood-fed (post 24 h adult female A. stephensi midgut tissue. Results We obtained 7061 and 8306 ESTs from the sugar-fed and P. yoelii infected mosquito midgut tissue libraries, respectively. ESTs from the combined dataset formed 1319 contigs and 2627 singlets, totaling to 3946 unique transcripts. Putative functions were assigned to 1615 (40.9% transcripts using BLASTX against UniProtKB database. Amongst unannotated transcripts, we identified 1513 putative novel transcripts and 818 potential untranslated regions (UTRs. Statistical comparison of annotated and unannotated ESTs from the two libraries identified 119 differentially regulated genes. Out of 3946 unique transcripts, only 1387 transcripts were mapped on the A. gambiae genome. These also included 189 novel transcripts, which were mapped to the unannotated regions of the genome. The EST data is available as ESTDB at http://mycompdb.bioinfo-portal.cdac.in/cgi-bin/est/index.cgi. Conclusion 3946 unique transcripts were successfully identified from the adult female A. stephensi midgut tissue. These data can be used for microarray development for better understanding of vector-parasite relationship and to study differences or similarities with other malaria vectors. Mapping of putative novel transcripts from A. stephensi on the A. gambiae genome proved fruitful in identification and annotation of several genes. Failure of some novel transcripts to map on the A. gambiae genome

  10. Gene discovery using massively parallel pyrosequencing to develop ESTs for the flesh fly Sarcophaga crassipalpis

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    Hahn Daniel A

    2009-05-01

    Full Text Available Abstract Background Flesh flies in the genus Sarcophaga are important models for investigating endocrinology, diapause, cold hardiness, reproduction, and immunity. Despite the prominence of Sarcophaga flesh flies as models for insect physiology and biochemistry, and in forensic studies, little genomic or transcriptomic data are available for members of this genus. We used massively parallel pyrosequencing on the Roche 454-FLX platform to produce a substantial EST dataset for the flesh fly Sarcophaga crassipalpis. To maximize sequence diversity, we pooled RNA extracted from whole bodies of all life stages and normalized the cDNA pool after reverse transcription. Results We obtained 207,110 ESTs with an average read length of 241 bp. These reads assembled into 20,995 contigs and 31,056 singletons. Using BLAST searches of the NR and NT databases we were able to identify 11,757 unique gene elements (ES. crassipalpis unigenes among GO Biological Process functional groups with that of the Drosophila melanogaster transcriptome suggests that our ESTs are broadly representative of the flesh fly transcriptome. Insertion and deletion errors in 454 sequencing present a serious hurdle to comparative transcriptome analysis. Aided by a new approach to correcting for these errors, we performed a comparative analysis of genetic divergence across GO categories among S. crassipalpis, D. melanogaster, and Anopheles gambiae. The results suggest that non-synonymous substitutions occur at similar rates across categories, although genes related to response to stimuli may evolve slightly faster. In addition, we identified over 500 potential microsatellite loci and more than 12,000 SNPs among our ESTs. Conclusion Our data provides the first large-scale EST-project for flesh flies, a much-needed resource for exploring this model species. In addition, we identified a large number of potential microsatellite and SNP markers that could be used in population and systematic

  11. DEVELOPMENT OF EST-SSR MARKERS TO ASSESS GENETIC DIVERSITY OF BROCCOLI AND ITS RELATED SPECIES

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    Nur Kholilatul Izzah

    2017-01-01

    Full Text Available Development of Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR markers derived from public database is known to be more efficient, faster and low cost. The objective of this study was to generate a new set of EST-SSR markers for broccoli and its related species and their usefulness for assessing their genetic diversity. A total of 202 Brassica oleracea ESTs were retrieved from NCBI and then assembled into 172 unigenes by means of CAP3 program. Identification of SSRs was carried out using web-based tool, RepeatMasker software. Afterwards, EST-SSR markers were developed using Primer3 program. Among the identified SSRs, trinucleotide repeats were the most common repeat types, which accounted for about 50%. A total of eight primer pairs were successfully designed and yielded amplification products. Among them, five markers were polymorphic and displayed a total of 30 alleles with an average number of six alleles per locus. The polymorphic markers were subsequently used for analyzing genetic diversity of 36 B. oleracea cultivars including 22 broccoli, five cauliflower and nine kohlrabi cultivars based on genetic similarity matrix as implemented in NTSYS program. At similarity coefficient of 61%, a UPGMA clustering dendrogram effectively separated 36 genotypes into three main groups, where 30 out of 36 genotypes were clearly discriminated. The result obtained in the present study would help breeders in selecting parental lines for crossing. Moreover, the novel EST-SSR markers developed in the study could be a valuable tool for differentiating cultivars of broccoli and related species.

  12. Strengths and weaknesses of EST-based prediction of tissue-specific alternative splicing

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    Vingron Martin

    2004-09-01

    Full Text Available Abstract Background Alternative splicing contributes significantly to the complexity of the human transcriptome and proteome. Computational prediction of alternative splice isoforms are usually based on EST sequences that also allow to approximate the expression pattern of the related transcripts. However, the limited number of tissues represented in the EST data as well as the different cDNA construction protocols may influence the predictive capacity of ESTs to unravel tissue-specifically expressed transcripts. Methods We predict tissue and tumor specific splice isoforms based on the genomic mapping (SpliceNest of the EST consensus sequences and library annotation provided in the GeneNest database. We further ascertain the potentially rare tissue specific transcripts as the ones represented only by ESTs derived from normalized libraries. A subset of the predicted tissue and tumor specific isoforms are then validated via RT-PCR experiments over a spectrum of 40 tissue types. Results Our strategy revealed 427 genes with at least one tissue specific transcript as well as 1120 genes showing tumor specific isoforms. While our experimental evaluation of computationally predicted tissue-specific isoforms revealed a high success rate in confirming the expression of these isoforms in the respective tissue, the strategy frequently failed to detect the expected restricted expression pattern. The analysis of putative lowly expressed transcripts using normalized cDNA libraries suggests that our ability to detect tissue-specific isoforms strongly depends on the expression level of the respective transcript as well as on the sensitivity of the experimental methods. Especially splice isoforms predicted to be disease-specific tend to represent transcripts that are expressed in a set of healthy tissues rather than novel isoforms. Conclusions We propose to combine the computational prediction of alternative splice isoforms with experimental validation for

  13. Estéticas no design de joias contemporâneas

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    Erika Yamamoto Lee

    2015-06-01

    Full Text Available O presente artigo refere-se à ação de produzir joias, na contemporaneidade, considerando o pensamento, o conhecimento e o sentimento do autor joalheiro. A história da joalheria e a da estética são relacionadas, ressaltando as mudanças sociológicas das principais rupturas escolásticas nas artes e na filosofia das formas. A atividade do designer de joias está atrelada aos estudos do mercado, da trajetória do belo, na sociedade, com o objetivo de encarnar na forma e sua essência no objeto estético. O conhecimento em profundidade sobre o belo e suas categorias estéticas seguem o seu tempo e mercado, permitindo ao designer liberar o caminho para o juízo crítico, a faculdade de pensar a forma, na fronteira entre teoria e prática, emoção e cálculo. A joia contemporânea reluz o belo, a técnica, a ideia, o imaginário. A estética contemporânea pondera a escassez de matéria-prima e os vários pensamentos que refletem na criação da forma. O conteúdo deste texto pincela brevemente a importância do conhecimento do designer no desenvolvimento de novas formas para atender às exigências do mercado.

  14. Surviving extreme polar winters by desiccation: clues from Arctic springtail (Onychiurus arcticus EST libraries

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    Kube Michael

    2007-12-01

    Full Text Available Abstract Background Ice, snow and temperatures of -14°C are conditions which most animals would find difficult, if not impossible, to survive in. However this exactly describes the Arctic winter, and the Arctic springtail Onychiurus arcticus regularly survives these extreme conditions and re-emerges in the spring. It is able to do this by reducing the amount of water in its body to almost zero: a process that is called "protective dehydration". The aim of this project was to generate clones and sequence data in the form of ESTs to provide a platform for the future molecular characterisation of the processes involved in protective dehydration. Results Five normalised libraries were produced from both desiccating and rehydrating populations of O. arcticus from stages that had previously been defined as potentially informative for molecular analyses. A total of 16,379 EST clones were generated and analysed using Blast and GO annotation. 40% of the clones produced significant matches against the Swissprot and trembl databases and these were further analysed using GO annotation. Extraction and analysis of GO annotations proved an extremely effective method for identifying generic processes associated with biochemical pathways, proving more efficient than solely analysing Blast data output. A number of genes were identified, which have previously been shown to be involved in water transport and desiccation such as members of the aquaporin family. Identification of these clones in specific libraries associated with desiccation validates the computational analysis by library rather than producing a global overview of all libraries combined. Conclusion This paper describes for the first time EST data from the arctic springtail (O. arcticus. This significantly enhances the number of Collembolan ESTs in the public databases, providing useful comparative data within this phylum. The use of GO annotation for analysis has facilitated the identification of a

  15. Marcuse e a crítica estética da modernidade – uma nova educação estética?

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    Ricardo Barbosa

    2014-10-01

    Full Text Available O artigo examina a leitura que Marcuse faz de Schiller, em relação à necessidade de uma educação estética. Para ambos a razão precisa estar ancorada na sensibilidade. Introduz-se também aqui a nocão da linguagem como mediadora. Abstract: The article examines the various ways in which Marcuse interpretates Schiller in relation to an aesthetic education. For both reason must be anchored upon sensibility. Here the question of language as mediator is introduced. Keywords: Aesthetic experience; reason; sensibility; language. Ricardo Barbosa é professor do Departamento de Filosofia da UERJ.

  16. Sequencing and analysis of full-length cDNAs, 5'-ESTs and 3'-ESTs from a cartilaginous fish, the elephant shark (Callorhinchus milii).

    KAUST Repository

    Brenner, Sydney

    2012-10-08

    Cartilaginous fishes are the most ancient group of living jawed vertebrates (gnathostomes) and are, therefore, an important reference group for understanding the evolution of vertebrates. The elephant shark (Callorhinchus milii), a holocephalan cartilaginous fish, has been identified as a model cartilaginous fish genome because of its compact genome (∼910 Mb) and a genome project has been initiated to obtain its whole genome sequence. In this study, we have generated and sequenced full-length enriched cDNA libraries of the elephant shark using the \\'oligo-capping\\' method and Sanger sequencing. A total of 6,778 full-length protein-coding cDNA and 10,701 full-length noncoding cDNA were sequenced from six tissues (gills, intestine, kidney, liver, spleen, and testis) of the elephant shark. Analysis of their polyadenylation signals showed that polyadenylation usage in elephant shark is similar to that in mammals. Furthermore, both coding and noncoding transcripts of the elephant shark use the same proportion of canonical polyadenylation sites. Besides BLASTX searches, protein-coding transcripts were annotated by Gene Ontology, InterPro domain, and KEGG pathway analyses. By comparing elephant shark genes to bony vertebrate genes, we identified several ancient genes present in elephant shark but differentially lost in tetrapods or teleosts. Only ∼6% of elephant shark noncoding cDNA showed similarity to known noncoding RNAs (ncRNAs). The rest are either highly divergent ncRNAs or novel ncRNAs. In addition to full-length transcripts, 30,375 5\\'-ESTs and 41,317 3\\'-ESTs were sequenced and annotated. The clones and transcripts generated in this study are valuable resources for annotating transcription start sites, exon-intron boundaries, and UTRs of genes in the elephant shark genome, and for the functional characterization of protein sequences. These resources will also be useful for annotating genes in other cartilaginous fishes whose genomes have been targeted for

  17. La metaphore est-elle la marque de l’insuffisance de l’esprit?

    Directory of Open Access Journals (Sweden)

    Isabelle Oliveira

    2010-01-01

    Full Text Available L’une des caractéristiques les plus communément reconnues à la discipline de la terminologie est sa conceptualité. Au contraire de l’opinion émise sur la métaphore, la terminologie s’efforce de travailler à partir de notions précises dont le sens doit être délimité et l’articulation à d’autres notions explicitée afin de bannir les ambiguïtés et les fausses évidences qu’elles véhiculent. Elle se distingue aussi du texte poétique caractérisé en particulier par la richesse des images et le jeu sur les signifiants. On comprend dès lors pourquoi la métaphore apparaît de prime abord comme une ennemie du champ de la terminologie qu’il faudrait bannir à tout prix. Cette figure de rhétorique consistant en un transport de sens risque à tout moment de rendre inopérant l’effort d’univocité et de rigueur du travail du terminologue. Mais la métaphore n’est pourtant pas absente des textes de spécialité. Il faut dès lors se demander si cette présence est « accidentelle”, contingente, ou si elle est incontournable et signifiante. Pour cela, il nous faudra étudier à la fois la place de la métaphore dans le texte de spécialité, et le discours des scientifiques portant sur elle. Mais une telle démarche nous laisse prisonnier de la dichotomie entre concept et métaphore et masque peutêtre une parenté inaperçue entre eux. Si le concept est habité par la métaphore, qu’en est-il du discours de spécialité? N’est-il pas réduit à n’être qu’un vaste poème qui ne s’avouerait pas comme tel? Est-elle seulement une figure de style ou aurait-elle un impact qui outrepasseraitsa modeste fonction discursive ? Il semble bien, même si le débat sur la métaphore et son usage est loin d’être clos. Notre théorie s’inscrit plutôt dans le sens d’une réévaluation du sens, d’une extension toujours plus grande de l’impact de ce trope qui, plus qu’une simple finalité esthétique, aurait une

  18. Entretelones de una "estética operatoria" Luis Juan Guerrero y Walter Benjamin

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    Luis Ignacio García

    2009-06-01

    Full Text Available En este artículo se ensaya una aproximación a la labor estético-filosófica del argentino Luis Juan Guerrero (1899-1957, a través de un cotejo de su temprana y productiva recepción de algunos aspectos centrales de la obra de Walter Benjamin. Se intenta mostrar que esta clave de lectura permite un acceso privilegiado a la labor de Guerrero, a la vez que ayuda a construir una imagen más compleja y matizada de la generación intelectual "antipositivista" a la que perteneció. La olvidada obra de Guerrero se compone principalmente de trabajos sobre ética, psicología y estética. Acotaremos nuestra atención a su principal trabajo, y acaso la obra sobre estética más ambiciosa jamás escrita en nuestro país: Estética Operatoria en sus Tres Direcciones. Se muestra en el artículo que la summa estética de Guerrero, si bien expresa con toda claridad las preocupaciones centrales de una generación intelectual marcada por la cultura alemana de entreguerras y por la problemática de la "crisis de la cultura", inscribe, a su vez, una anomalía -benjaminiana-, una inflexión político-materialista, que pone en cuestión la idea frecuente de que la "crisis de la cultura" una y otra vez diagnosticada en aquella época mostró siempre alternativas "espiritualistas" de resolución.This essay deals with the aesthetic-philosophical work of the Argentinean Luis Juan Guerrero (1899-1957, through a consideration of its early and fruitful reception of some central aspects of Walter Benjamin's work. It is shown that this perspective allows a privileged access to Guerrero's work, at the same time that it makes more complex the image of the "anti-positivistic" intellectual generation in Argentina. Guerrero's forgotten work includes principally contributions on ethics, psychology and aesthetics. This essay will pay attention only to his most important work, maybe the most ambitious aesthetic work ever written in Argentina: Operatory Aesthetics in its Three

  19. Desartificação da arte e construtos estético-sociais

    Directory of Open Access Journals (Sweden)

    Rodrigo Duarte

    2012-09-01

    Full Text Available Este artigo pretende examinar a relação entre a concepção de desartificação (Entkunstung, de Theodor Adorno, e a minha própria noção de “construto estético-social”. A primeira se refere à situação da arte numa época dominada pela indústria cultural e suas mercadorias, na qual, sob certas circunstâncias, os próprios artistas reduzem sua expressão a uma estrutura mínima a fim de prevenir as ameaças à sua arte por parte do “mundo administrado” (Adorno. Já o conceito de “construto estético-social é uma tentativa de compreender alguns fenômenos estéticos contemporâneos que apresentam, por um lado, traços de mercadorias culturais, já que renunciam à complexidade formal das obras de arte propriamente ditas e são – pelo menos parcialmente – veiculados por típicos meios de massa; por outro lado, eles não se enquadram totalmente na categoria de mercadoria cultural, uma vez que apresentam aspectos críticos à sociedade tardo-capitalista e são ligados a ações políticas originadas em comunidades muito pobres, que se entendem a si mesmas como dispostas a transformar aquela sociedade. Fenômenos que se enquadram na descrição de “construtos estético-sociais” podem ser encontrados, por exemplo, na cultura hip hop. A conclusão preliminar a que se chega é que, apesar de a arte desertificada e os “construtos estético-sociais” não serem exatamente a mesma coisa, há uma ligação subterrânea entre essas duas concepções, uma vez que ambas operam – de modo mais ou menos intencional – uma redução na forma de sua expressão a fim de fazer frente às exigências de uma cultura contemporânea autêntica.

  20. El maestro: un artesano del lenguaje. Escritura y experiencia estética de la palabra

    Directory of Open Access Journals (Sweden)

    Claudia Arcila Rojas

    2015-06-01

    Full Text Available La identidad de una obra expresada a través de la escritura se teje en las voces y esfuerzos que se encarnan en memoria y dejan huella en el relato. El maestro es acción de este lenguaje movido por el historial social y cultural que permite hacer de la escritura un oficio ético y estético donde la mano construye y nombra las emociones que atraviesan al cuerpo. La escritura es el testimonio vital del maestro como artesano; es el arte del lenguaje que sale de su silencio con la lectura del relato, pero es, además, la potencia del pensamiento puesto en pretexto estético donde se pregunta y se reflexiona por el tiempo y por el espacio de la palabra, es decir, las cronologías, kairologías, territorialidades y escenarios de las esferas educativas. Sobre estos presupuestos se propone una discusión pedagógica en torno a la necesidad de pensar la escritura artesanal como intención estética y política donde se plasma el testimonio del compromiso ético del maestro, pero también la perspectiva de los nuevos retos que la educación debe asumir para reencontrar el lenguaje con la vida y con las herencias socioculturales que dan cuenta del sujeto y de las subjetividades que confluyen en los procesos de formación, y que tienen despliegue y búsqueda en el escenario creativo y productivo de la escritura. Se intentará, entonces, reivindicar una pedagogía de la posibilidad en el lenguaje, una narratividad donde la memoria traza un puente para el encuentro de épocas, de rostros, de acontecimientos, de silencios y de saberes, donde el maestro está llamado a tejer, tallar, dibujar, componer y comunicar las artesanías que la educación ha olvidado, es decir, recuperar la memoria estética, lo cual también implica un olvido de las formas hegemónicas del arte.

  1. annot8r: GO, EC and KEGG annotation of EST datasets

    Directory of Open Access Journals (Sweden)

    Schmid Ralf

    2008-04-01

    Full Text Available Abstract Background The expressed sequence tag (EST methodology is an attractive option for the generation of sequence data for species for which no completely sequenced genome is available. The annotation and comparative analysis of such datasets poses a formidable challenge for research groups that do not have the bioinformatics infrastructure of major genome sequencing centres. Therefore, there is a need for user-friendly tools to facilitate the annotation of non-model species EST datasets with well-defined ontologies that enable meaningful cross-species comparisons. To address this, we have developed annot8r, a platform for the rapid annotation of EST datasets with GO-terms, EC-numbers and KEGG-pathways. Results annot8r automatically downloads all files relevant for the annotation process and generates a reference database that stores UniProt entries, their associated Gene Ontology (GO, Enzyme Commission (EC and Kyoto Encyclopaedia of Genes and Genomes (KEGG annotation and additional relevant data. For each of GO, EC and KEGG, annot8r extracts a specific sequence subset from the UniProt dataset based on the information stored in the reference database. These three subsets are then formatted for BLAST searches. The user provides the protein or nucleotide sequences to be annotated and annot8r runs BLAST searches against these three subsets. The BLAST results are parsed and the corresponding annotations retrieved from the reference database. The annotations are saved both as flat files and also in a relational postgreSQL results database to facilitate more advanced searches within the results. annot8r is integrated with the PartiGene suite of EST analysis tools. Conclusion annot8r is a tool that assigns GO, EC and KEGG annotations for data sets resulting from EST sequencing projects both rapidly and efficiently. The benefits of an underlying relational database, flexibility and the ease of use of the program make it ideally suited for non

  2. Use of EST database markers from M. truncatula in the transferability to other forage legumes.

    Science.gov (United States)

    Chandra, Amaresh

    2011-05-01

    In general tropical forage legumes lack microsatellites or simple sequence repeat (SSR) markers. Development of genic SSR markers from expressed sequence tagged (EST) database is an alternate and efficient approach to generate the standard DNA markers for genome analysis of such crop species. In the present paper a total of 816 EST-SSRs containing perfect repeats of mono (33.5%), di (14.7%), tri (39.3%), tetra (2.7%), penta (0.7%) and hexa (0.4%) nucleotides were identified from 1,87,763 ESTs of Medicago truncatula. Along with, 70 (8.5%) SSRs of a compound type were also observed. Seven primer pairs of tri repeats were tested for cross transferability in 19 accessions of forage legumes comprising 11 genera. At two different annealing temperatures (55 and 60 degreesC) all primer pairs except AJ410087 reacted with many accessions of forage legumes. Atotal of 51 alleles were detected with six M. truncatula EST-SSRs primer-pairs against DNAfrom 19 accessions representing 11 genera where number of alleles ranged from 2 to 13. The cross-transferability of these EST-SSRs was 40.6% at 55 degreesC and 32.3% at 60 degreesC annealing temperature. 24 alleles of the total 50 (48%) at 55 degreesC and 27 of 51 (53%) at 60 degreesC were polymorphic among the accessions. These 27 polymorphic amplicons identified could be used as DNA markers. This study demonstrates the developed SSR markers from M. truncatula ESTs as a valuable genetic markers and also proposes the possibility of transferring these markers between species of different genera of the legumes of forage importance. It was evident from the results obtained with a set of Desmanthus virgatus accessions where SequentialAgglomerative Hierarchical and Nested (SAHN) cluster analysis based on Dice similarity and Unweighted Pair Group Method with Arithmetic mean Algorithm (UPGMA) revealed significant variability (24 to 74%) among the accessions. High bootstrap values (>30) supported the nodes generated by dendrogram analysis of

  3. Expressed sequence tags (ESTs from immune tissues of turbot (Scophthalmus maximus challenged with pathogens

    Directory of Open Access Journals (Sweden)

    Lemos Manuel L

    2008-09-01

    Full Text Available Abstract Background The turbot (Scophthalmus maximus; Scophthalmidae; Pleuronectiformes is a flatfish species of great relevance for marine aquaculture in Europe. In contrast to other cultured flatfish, very few genomic resources are available in this species. Aeromonas salmonicida and Philasterides dicentrarchi are two pathogens that affect turbot culture causing serious economic losses to the turbot industry. Little is known about the molecular mechanisms for disease resistance and host-pathogen interactions in this species. In this work, thousands of ESTs for functional genomic studies and potential markers linked to ESTs for mapping (microsatellites and single nucleotide polymorphisms (SNPs are provided. This information enabled us to obtain a preliminary view of regulated genes in response to these pathogens and it constitutes the basis for subsequent and more accurate microarray analysis. Results A total of 12584 cDNAs partially sequenced from three different cDNA libraries of turbot (Scophthalmus maximus infected with Aeromonas salmonicida, Philasterides dicentrarchi and from healthy fish were analyzed. Three immune-relevant tissues (liver, spleen and head kidney were sampled at several time points in the infection process for library construction. The sequences were processed into 9256 high-quality sequences, which constituted the source for the turbot EST database. Clustering and assembly of these sequences, revealed 3482 different putative transcripts, 1073 contigs and 2409 singletons. BLAST searches with public databases detected significant similarity (e-value ≤ 1e-5 in 1766 (50.7% sequences and 816 of them (23.4% could be functionally annotated. Two hundred three of these genes (24.9%, encoding for defence/immune-related proteins, were mostly identified for the first time in turbot. Some ESTs showed significant differences in the number of transcripts when comparing the three libraries, suggesting regulation in response to these

  4. GarlicESTdb: an online database and mining tool for garlic EST sequences

    Directory of Open Access Journals (Sweden)

    Choi Sang-Haeng

    2009-05-01

    Full Text Available Abstract Background Allium sativum., commonly known as garlic, is a species in the onion genus (Allium, which is a large and diverse one containing over 1,250 species. Its close relatives include chives, onion, leek and shallot. Garlic has been used throughout recorded history for culinary, medicinal use and health benefits. Currently, the interest in garlic is highly increasing due to nutritional and pharmaceutical value including high blood pressure and cholesterol, atherosclerosis and cancer. For all that, there are no comprehensive databases available for Expressed Sequence Tags(EST of garlic for gene discovery and future efforts of genome annotation. That is why we developed a new garlic database and applications to enable comprehensive analysis of garlic gene expression. Description GarlicESTdb is an integrated database and mining tool for large-scale garlic (Allium sativum EST sequencing. A total of 21,595 ESTs collected from an in-house cDNA library were used to construct the database. The analysis pipeline is an automated system written in JAVA and consists of the following components: automatic preprocessing of EST reads, assembly of raw sequences, annotation of the assembled sequences, storage of the analyzed information into MySQL databases, and graphic display of all processed data. A web application was implemented with the latest J2EE (Java 2 Platform Enterprise Edition software technology (JSP/EJB/JavaServlet for browsing and querying the database, for creation of dynamic web pages on the client side, and for mapping annotated enzymes to KEGG pathways, the AJAX framework was also used partially. The online resources, such as putative annotation, single nucleotide polymorphisms (SNP and tandem repeat data sets, can be searched by text, explored on the website, searched using BLAST, and downloaded. To archive more significant BLAST results, a curation system was introduced with which biologists can easily edit best-hit annotation

  5. ¿Estás nervioso? Las elecciones desde una villa del Gran Buenos Aires

    Directory of Open Access Journals (Sweden)

    María Cecilia Ferraudi Curto

    2011-11-01

    Full Text Available En este artículo pretendo explorar la política en el Gran Buenos Aires a partir de un análisis de las elecciones legislativas de 2009 desde mi etnografía en una villa de La Matanza en proceso de urbanización. A partir de allí, intentaré dar cuenta de una serie de perspectivas que quedan opacadas en los análisis centrados en procesos electorales. Mientras éstos enfocan hacia las estrategias de campaña de los candidatos y los entramados políticos desde los cuales construyen apoyos para explicar los resultados electorales (o intentar predecirlos, el punto central de este artículo consiste en mostrar cómo las elecciones se imponen y son apropiadas localmente a partir de una perspectiva centrada en la urbanización de la villa.

  6. A estética realista dos filmes sobre a ditadura militar no Brasil

    Directory of Open Access Journals (Sweden)

    Helena Stigger

    2008-12-01

    Full Text Available O cinema canaliza em seu aporte uma impressão de realidade baseada na experiência empírica do realizador e da sociedade ao qual ele pertence. Em sua condição de discurso, o cinema, assim como todas as formas narrativas, abrange um enunciado e um espectador. Diferentemente de um romance, a “gramática” cinematográfica é composta por um conjunto de elementos como a imagem, som, música entre outros que, organizados, contam uma história. Entretanto, a forma como estes componentes são gravados em imagens e ordenados numa seqüência narrativa não é única. Desta forma, podemos pensar qual é o propósito de uma escolha estética usual nos filmes Pra frente Brasil e Batismo de Sangue para representar a Ditadura Militar Brasileira.

  7. RIEN N’EST FIXE: L’EXPÉRIENCE-NIETZSCHE ET LE QUOTIDIEN ÉDUCATIONNEL

    Directory of Open Access Journals (Sweden)

    Luciano Bedin da Costa

    2011-12-01

    Full Text Available Cet article est une lecture du quotidienne éducationnel par la philosophie de riedrich Nietzsche (1844-1900 avec des concepts tels quels : expérience, nihilisme, ressentiment, mauvaise conscience, force et de la volonté de puissance. La théorie de Nietzsche est utilisé comme un outil de réflexion sur les pratiques quotidiennes vécu dans l'éducation, de rompre avec l'inertie du discours des pleurs et nihiliste. Ce que nous appelons l'expérience de Nietzsche implique un autre regard, une grande santé et une éthique pour une évasion de la cristallisation et production des différences. MOTS-CLÉS : expérience; Nietzsche, nihilisme, formation, volonté de puissance

  8. A Centaur in Salaryman's Clothing: Parody and Play in est em's Centaur Manga

    Directory of Open Access Journals (Sweden)

    Anne Lee

    2016-07-01

    Full Text Available Japanese manga artist est em (esu to emu is notable for blurring genre boundaries and subverting established conventions in various publications since her debut in 2006. Two of her works, Hatarake, kentaurosu! (‘Work, Centaur!’ and equus, focus exclusively on male centaurs in homosocial settings. Classified as shōjo (girls’ manga and BL (‘boys’ love’ manga respectively, these two works allow female readers to enjoy the pleasures of homoerotic subtexts and intertextual parody. This paper examines how conventions of sexuality and gender, particularly hegemonic masculinity and heterosexuality, are constructed/deconstructed in est em’s centaur manga using the framework of intertextuality, with particular emphasis on parody, pleasure and play. By placing centaurs in realistic, everyday settings, these works present a critique of Japan’s contemporary salaryman culture, while also highlighting issues of alienation and otherness that both female readers and gay men face in their daily lives.

  9. Sobre la educación estética en el ámbito familiar

    OpenAIRE

    Urpí-Guercia, C. (Carmen); Naval, C. (Concepción)

    2006-01-01

    En 2004, la ONU conmemoró el Año Internacional de la Familia. Es nuestro interés tratar aquí un aspecto de la educación con frecuencia inescuchado (la educación estética) en un ámbito educativo primordial como es la familia, y a menudo también pasado por alto o dado por supuesto, centrándonos especialmente en la experiencia del cine como colaboradora de la educación estética. Resaltamos la relevancia del estilo familiar de vida, los hábitos elementales de higiene, vestimenta y alimentación, e...

  10. Da estética ao terrorismo: Agamben, entre Nietzsche e Heidegger

    Directory of Open Access Journals (Sweden)

    Cláudio Oliveira

    2012-09-01

    Full Text Available O artigo tenta demonstrar que, em seu primeiro livro, O homem sem conteúdo, Giorgio Agamben se move entre a ideia heideggeriana de uma destruição da estética e a ideia nietzschiana de uma destruição da estética do expectador. O artigo mostra como Agamben reduz estas duas ideias, que na origem são distintas, em uma única e mesma ideia. O artigo tenta ainda demonstrar como Agamben, partindo dessa discussão com Nietzsche e Heidegger, chega a uma ideia própria da arte, em nosso tempo, como possuindo um caráter terrorista.

  11. A bela alma e a estética goethiana do símbolo

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    Wilma Patricia Maas

    2011-08-01

    Full Text Available Pretende-se acompanhar o desenvolvimento do pensamento estético de Goethe entre os anos de 1780 e 1790 paralelamente a sua expressão no livro VI do romance Os anos de aprendizado de Wilhelm Meister (1795-1796, no qual se narra a trajetória da Bela Alma em direção ao progressivo abandono dos símbolos religiosos como suporte sensível da fé. Entende-se que a convivência de Goethe com Karl Philipp Moritz foi decisiva para a concepção dos ensaios “Sobre os objetos das artes plásticas” (1797 e “Imitação simples, maneira, estilo” (1789, fundamentais para a constituição do pensamento estético de Goethe à época.

  12. Quel est l'impact des business cycles sur les performances des fonds de Private Equity

    OpenAIRE

    Lauber, Jérôme; Seiler, Robert

    2018-01-01

    Les actifs financiers cotés sont sensibles aux cycles économiques et aux aléas des autres classes d’actifs, mais qu’en est-il pour les actifs financiers non-cotés ? Ce travail s’intéresse aux performances des fonds de Private Equity, plus précisément aux fonds Buyout et Secondary aux Etats-Unis, et à leur sensibilité à des facteurs exogènes aux fonds tels que les données macroéconomiques et la performance des marchés publics. L’objectif est tout d’abord de définir quels facteurs exogènes au f...

  13. La dimensión estética del poder mediático

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    Ximena González

    2009-05-01

    Full Text Available El poder de los medios de comunicación y información es, en la mayoría de los casos, analizado a partir de larelación comando-obediencia, es decir como poder coercitivo. Esto conlleva a centrarse en los efectos de dicho poder, yno en lo que hace la particularidad del poder mediático en la reestructuración de las sociedades actuales. Este artículoplantea caracterizar este poder mediático - multiplicado por el desarrollo masivo de las tecnologías de informacióny comunicación - ya no únicamente como poder sobre, sino como poder simbólico, es decir como poder meramenteestético.

  14. « Tout homme est un prophète »

    Directory of Open Access Journals (Sweden)

    Anne-Cécile Pottier-Thoby

    2000-05-01

    Full Text Available Ecrivain de la spiritualité chrétienne, Julien Green, grand lecteur de la Bible, de Blake et de Léon Bloy, voit dans le romancier une sorte de prophète capable de déchiffrer les signes du temps, et "la trame invisible de l'existence". Le Journal de l'écrivain est aussi ponctué d'attentions aux présages, et aux signes annonciateurs. Dans un de ses romans, Varouna, c'est même tout le récit qui s'appuie sur un rêve prophétique. Mais Julien Green prend soin de distinguer les prophètes des faux-prophètes, comme les psychanalystes.

  15. Leer lo estético desde lo político

    OpenAIRE

    Verónica Peñafiel

    2013-01-01

    En el Ecuador, y específicamente en la vida política del país, el teatro ha jugado un papel más importante del que se le ha reconocido. Pensar lo estético relacionado con lo político, puede ser una interesante clave de lectura para el teatro quiteño y, me arriesgo a afirmar, para el teatro a secas. Este análisis no se lo hará desde el teatro político, se pretende, más bien, analizar la dimensión política del mismo y de la relación entre teatro y política. Dicha dimensión política ha tomado di...

  16. EST analysis of gene expression in the tentacle of Cyanea capillata.

    Science.gov (United States)

    Yang, Yanzhen; Cun, Shujian; Xie, Xiaojin; Lin, Jianghai; Wei, Jianwen; Yang, Wenli; Mou, Chunyan; Yu, Cuiling; Ye, Lanting; Lu, Yang; Fu, Zhiyan; Xu, Anlong

    2003-03-13

    Jellyfish, Cyanea capillata, has an important position in head patterning and ion channel evolution, in addition to containing a rich source of toxins. In the present study, 2153 expressed sequence tags (ESTs) from the tentacle cDNA library of C. capillata were analyzed. The initial ESTs consisted of 198 clusters and 818 singletons, which revealed approximately 1016 unique genes in the data set. Among these sequences, we identified several genes related to head and foot patterning, voltage-dependent anion channel gene and genes related to biological activities of venom. Five kinds of proteinase inhibitor genes were found in jellyfish for the first time, and some of them were highly expressed with unknown functions.

  17. Oeste frente a Este: Globalización temprana y gran divergencia

    OpenAIRE

    Rafael Dobado González; Alfredo García Hiernaux; David Guerrero Burbano

    2013-01-01

    Este trabajo expande nuestra investigación previa sobre la integración del mercado de granos en Europa y América (Dobado, García-Hiernaux y Guerrero, 2012). Usando la misma metodología econométrica, presentamos ahora: 1) la búsqueda de evidencia estadística en el Este de una “Globalización temprana” semejante a la encontrada en el Oeste desde mediados del siglo XVIII; 2) un estudio de la integración de los mercados en china y Japón y su funcionamiento en comparación con los países occidentale...

  18. La guerre contre les drogues illicites : Est-ce qu’elle est perdue ? - Has the war against drugs been lost ? - La guerra contro le droghe è persa ?

    Directory of Open Access Journals (Sweden)

    Dominick M.

    2009-08-01

    Full Text Available Le système légal du Mexique est dans un état de ruine à cause des cartels de la drogue soutenus et subventionnés par les États-Unis d'Amerique. En effet, les États-Unis sont le marché principal de la marijuana, de la cocaine et, en moindre quantité, des drogues synthétiques provénantes du Mexique. L’article met en évidence que les régions frontalières du Mexique sont pris en otage entre d’une part, les principaux producteurs mondiaux de coca et d’autre part, les principaux consommateurs (de l’Amerique du Nord et de l’Europe. Pour cela, l’avis de l’autrice et de son collaborateur est que le Mexique ne peut résoudre seul les problèmes liés au narcotrafique. The Mexican legal system is in a state of ruin because of illegal drug cartels supported, sustained and subsidized by the United States of America. It is the U.S. which is the market for marijuana, cocaine and to a lesser extent synthetic drugs trafficked through Mexico. This article evidences that especially the frontier regions of Mexico have been taken hostage between, on the one hand, the principal world producers of cocaine and, on the other hand, the principal consumers (from North America and from Europe. Accordingly, it is the view of the author and her collaborator that Mexico cannot resolve in isolation the problems involved in narco-trafficking.Il sistema legale messicano versa in uno stato disastroso a causa dei cartelli della droga sostenuti e sovvenzionati dagli Stati Uniti d’America. Infatti, gli USA rappresentano il principale mercato della marijuana, della cocaina e delle droghe sintetiche (queste ultime in misura minore provenienti dal Messico. L’articolo mette in evidenza che le regioni frontaliere del Messico sono prese in ostaggio, da una parte, dai principali produttori modiali di cocaina e, dall’altra, dai principali consumatori (che si trovano nell’America del Nord ed in Europa. Pertanto, secondo l’autrice ed il suo collaboratore

  19. Le salaire minimum n'est pas toujours le meilleur moyen d'accroître ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    4 mai 2016 ... La hausse du salaire minimum a longtemps été considérée, pour la plupart des pays, comme un moyen de protéger les travailleurs pauvres et leur famille. De fait, cette intervention vigoureuse sur le marché du travail est une mesure de protection sociale à laquelle ont recours beaucoup de pays ...

  20. La pisciculture en cage est prisée au Népal | CRDI - Centre de ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    25 oct. 2010 ... Des chercheurs du Nepal Agricultural Research Council ont constaté que l'eau du réservoir créé par le barrage se prêtait exceptionnellement bien à l'élevage des poissons, en particulier des carpes, dans de grandes cages flottantes. Le milieu est idéal pour les poissons car le plancton y abonde.

  1. Pays en transition : pour que les sociétés se transforment, il est ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Conscient du fait que les périodes de transformation risquent de provoquer le chaos, mais qu'elles sont également porteuses de rapides progrès sur le plan social et économique, le CRDI est intervenu dans quelque 25 pays ayant amorcé une transition de la guerre à la paix, de la dictature à la démocratie ou d'une ...

  2. Identification de Staphylococcus aureus : est-il possible de réduire ...

    African Journals Online (AJOL)

    Dans le contexte de pays moins avancé comme le Bénin, cela est un premier jet en vue de l'efficience des méthodes de diagnostic au laboratoire d'analyses biomédicales. Mots-clés : staphylocoagulase libre, plasma de lapin, efficience. ABSTRACT. Objective: The high cost of lyophilized rabbit's plasma, which is used for ...

  3. Regard critique sur On Est Ensemble: 852 mots pour comprendre le ...

    African Journals Online (AJOL)

    Résumé: L'ouvrage intitulé On Est Ensemble: 852 mots pour comprendre le français du Gabon (L. Ditougou, 2009) constitue une contribution importante dans l'histoire du français parlé au Gabon. Cette œuvre présente cependant quelques insuffisances sur le plan métalexicographique. Celles-ci portent sur les pré-textes, ...

  4. El gusto estético en la globalización

    Directory of Open Access Journals (Sweden)

    Carlos Fajardo Fajardo

    2014-09-01

    Full Text Available ¿Cómo opera el proceso del gusto y de la emoción estética en la disgregación entre el sujeto y el objeto posmodernos? La pluralidad, la heterogeneidad y lo multi-procesal conforman el corpus del juicio de gusto actual. Su resultado es la construcción de otros tipos de figuras del mundo, nuevas imágenes de lo real, diversas y distintas, creadas a partir no de la unidad y universalidad del placer estético clásico y dieciochesco, sino por medio de una emoción intersubjetiva y multiforme. El gusto es víctima del tiempo. De lo interesante estético burgués se ha pasado a lo impactante y espectacularizado posindustrial. Entonces, lo light, la alta costura, el turismo, el “mundo del arte”, la word music, la publicidad, los diseños del hogar, la farándula, los artistas jet, la literatura de autoayuda y de intimidades de famosos son las nuevas esferas de un gusto que ha puesto contra la pared todas las antiguas competencias de críticos de arte modernos y del público lector ilustrado en general. ¿Qué tipo de gusto actualmente ejercitamos?, o bien, ¿de qué manera hemos mutado el juicio de gusto en disgusto fragmentado, indescible, descentrado? Este ensayo contribuye a dar algunas respuestas a estos interrogantes.

  5. Il est temps que le Canada rende la justice accessible à tous | CRDI ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Il est temps que le Canada rende la justice accessible à tous. Adrian Di Giovanni, Lotta Teale, Vivek Maru. 29 juin 2016. Des étudiantes à l'école secondaire Female Experimental High School à Herat en Afghanistan. Photo : Graham Crouch / Banque mondiale. Lorsqu'on prive un enfant d'accès à l'école ou qu'on exclut ...

  6. The mining of toxin-like polypeptides from EST database by single residue distribution analysis

    Science.gov (United States)

    2011-01-01

    Background Novel high throughput sequencing technologies require permanent development of bioinformatics data processing methods. Among them, rapid and reliable identification of encoded proteins plays a pivotal role. To search for particular protein families, the amino acid sequence motifs suitable for selective screening of nucleotide sequence databases may be used. In this work, we suggest a novel method for simplified representation of protein amino acid sequences named Single Residue Distribution Analysis, which is applicable both for homology search and database screening. Results Using the procedure developed, a search for amino acid sequence motifs in sea anemone polypeptides was performed, and 14 different motifs with broad and low specificity were discriminated. The adequacy of motifs for mining toxin-like sequences was confirmed by their ability to identify 100% toxin-like anemone polypeptides in the reference polypeptide database. The employment of novel motifs for the search of polypeptide toxins in Anemonia viridis EST dataset allowed us to identify 89 putative toxin precursors. The translated and modified ESTs were scanned using a special algorithm. In addition to direct comparison with the motifs developed, the putative signal peptides were predicted and homology with known structures was examined. Conclusions The suggested method may be used to retrieve structures of interest from the EST databases using simple amino acid sequence motifs as templates. The efficiency of the procedure for directed search of polypeptides is higher than that of most currently used methods. Analysis of 39939 ESTs of sea anemone Anemonia viridis resulted in identification of five protein precursors of earlier described toxins, discovery of 43 novel polypeptide toxins, and prediction of 39 putative polypeptide toxin sequences. In addition, two precursors of novel peptides presumably displaying neuronal function were disclosed. PMID:21281459

  7. Analysis of cassava (Manihot esculenta) ESTs: A tool for the discovery of genes

    International Nuclear Information System (INIS)

    Zapata, Andres; Neme, Rafik; Sanabria, Carolina; Lopez, Camilo

    2011-01-01

    Cassava (Manihot esculenta) is the main source of calories for more than 1,000 millions of people around the world and has been consolidated as the fourth most important crop after rice, corn and wheat. Cassava is considered tolerant to abiotic and biotic stress conditions; nevertheless these characteristics are mainly present in non-commercial varieties. Genetic breeding strategies represent an alternative to introduce the desirable characteristics into commercial varieties. A fundamental step for accelerating the genetic breeding process in cassava requires the identification of genes associated to these characteristics. One rapid strategy for the identification of genes is the possibility to have a large collection of ESTs (expressed sequence tag). In this study, a complete analysis of cassava ESTs was done. The cassava ESTs represent 80,459 sequences which were assembled in a set of 29,231 unique genes (unigen), comprising 10,945 contigs and 18,286 singletones. These 29,231 unique genes represent about 80% of the genes of the cassava's genome. Between 5% and 10% of the unigenes of cassava not show similarity to any sequences present in the NCBI database and could be consider as cassava specific genes. a functional category was assigned to a group of sequences of the unigen set (29%) following the Gene Ontology Vocabulary. the molecular function component was the best represented with 43% of the sequences, followed by the biological process component (38%) and finally the cellular component with 19%. in the cassava ESTs collection, 3,709 microsatellites were identified and they could be used as molecular markers. this study represents an important contribution to the knowledge of the functional genomic structure of cassava and constitutes an important tool for the identification of genes associated to agricultural characteristics of interest that could be employed in cassava breeding programs.

  8. En quoi est-ce que cela aidera Veronica ? | CRDI - Centre de ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    5 nov. 2010 ... Il est fier que « chaque dispensaire du district de Rakai ait un ordinateur ». Il raconte comment, dans la deuxième année seulement de mise à l'essai de ce nouveau système, ils ont déjà empêché une épidémie de paludisme. Leurs rapports de surveillance des maladies transmis en temps opportun ont ...

  9. Et le gagnant est... Brendan Baker en entrevue | CRDI - Centre de ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    2 févr. 2011 ... Plus les Canadiens verront d'exemples positifs, plus ils comprendront les difficultés que doivent surmonter les populations des pays en développement, et plus ils éprouveront du souci et de l'empathie à leur égard. Si le piège de la pauvreté persiste encore, c'est entre autres parce que les gens d'ici ...

  10. Le big bang n'est pas une théorie comme les autres

    CERN Document Server

    Bonnet-Bidaud, Jean-Marc; Leglu, Dominique; Reinisch, Gilbert

    2009-01-01

    Le big bang n'est pas une théorie comme les autres. Ce n'est d'ailleurs pas une théorie physique au sens propre du terme, mais un scénario cosmologique issu des équations de la relativité générale. Il est le modèle qui s'ajuste le mieux aux observations actuelles, mais à quel prix ? Il nous livre un Univers composé à 96 % de matière et d'énergie noires inconnues. C'est donc un euphémisme que de dire que le big bang semble poser autant - sinon plus - de questions qu'il n'en résout. En ce sens, le big bang apparaît davantage comme une paramétrisation de notre ignorance plutôt que comme une modélisation d'un phénomène. Pourtant, le succès du big bang et l'adhésion qu'il suscite, tant dans la sphère scientifique que dans la sphère médiatique, ne se démentent pas. Surmédiatisé, son statut dépasse celui de modèle théorique, et la simple évocation de son nom suffit pour justifier des opérations de marketing scientifique ou rejeter des cosmologies alternatives. Pour éclaircir les pr...

  11. Novel and Stress Relevant EST Derived SSR Markers Developed and Validated in Peanut.

    Directory of Open Access Journals (Sweden)

    Tejas C Bosamia

    Full Text Available With the aim to increase the number of functional markers in resource poor crop like cultivated peanut (Arachis hypogaea, large numbers of available expressed sequence tags (ESTs in the public databases, were employed for the development of novel EST derived simple sequence repeat (SSR markers. From 16424 unigenes, 2784 (16.95% SSRs containing unigenes having 3373 SSR motifs were identified. Of these, 2027 (72.81% sequences were annotated and 4124 gene ontology terms were assigned. Among different SSR motif-classes, tri-nucleotide repeats (33.86% were the most abundant followed by di-nucleotide repeats (27.51% while AG/CT (20.7% and AAG/CTT (13.25% were the most abundant repeat-motifs. A total of 2456 EST-SSR novel primer pairs were designed, of which 366 unigenes having relevance to various stresses and other functions, were PCR validated using a set of 11 diverse peanut genotypes. Of these, 340 (92.62% primer pairs yielded clear and scorable PCR products and 39 (10.66% primer pairs exhibited polymorphisms. Overall, the number of alleles per marker ranged from 1-12 with an average of 3.77 and the PIC ranged from 0.028 to 0.375 with an average of 0.325. The identified EST-SSRs not only enriched the existing molecular markers kitty, but would also facilitate the targeted research in marker-trait association for various stresses, inter-specific studies and genetic diversity analysis in peanut.

  12. Regional characterization of suspected land Punta del Este ( east of the Republica Oriental del Uruguay )

    International Nuclear Information System (INIS)

    Preciozzi, F.; Sanchez Bettucci, L.; Oyhantcabal, P.; Pecoits, E.; Aubet, N.; Peel, E.; Basei, M.

    2003-01-01

    Field Suspect Punta del Este (TSPE) is located in the easternmost portion of Uruguay (Figure 1). This is bounded to the west by Shear Zone Punta del Este-Cordillera (Masquelin, 1990; Preciozzi et al., 1999; 2002), while the east with Rocha makes Formation (Hasui et al, 1975;.. Sanchez-Bettucci and Mezzano, 1993). The TSPE is represented by gneisses and Migmatites formed in the range of 1100 and 630 Ma (Preciozzi et al, in prep.) and have been intensively reworked during Rio Doce orogeny (ca. 600 Ma). Field Suspect Punta del Este (TSPE) is separated from the Neoproterozoic granitoids or granitoids by central Shear Zone Punta del Este-Cordillera represented by a thin strip of mylonite s and gneisses mylonitic able to anatexis. Granitoids are sub alkaline trend throughout of this guideline. The mylonitic foliation has a general direction between N15ºE and N40ºE with sub vertical slope where development ductile quartz and muscovite bands observed (Figure 2). The base of TSPE consists essentially of porphyritic granitoids deformed with biotite and muscovite, and ortho gneisses oftalmíticos granitoids, showing an attenuation of that reaching isotropic deformation terms. From studies in zircons from a sample of ortho gneisses (UCUR 03) by U / Pb conventional and SHRIMP ages of 753 ± 14 Ma for the edge obtained (consistent with the conventional age) and 1000 Ma for zircon center. This has allowed us to recognize in the protoliths one Grenvillian heritage in this area, preserved during crustal melting processes. Also, new U / Pb (Preciozzi et al., In prep.) Data show a orogénesis penicontemporánea to the Brasiliano cycle would be responsible for most of the generation of the rocks that make up the TSPE

  13. 23. X avati Pärnu muuseumis näitus "EST-design"

    Index Scriptorium Estoniae

    2002-01-01

    Väljapanek annab ülevaate eesti tootedisaini hetkeseisust ja tutvustab eesti disaini ekspordiks loodud kaubamärki. EST-designi kontseptsiooni on välja töötanud Toivo Rande, märgi on kujundanud Sven Sõrmus. Näitusel esinevad M. Grünberg, E. Jürgenson, T. Luisk, T. Mang, G. Nahkur, A. Puuraid, M. Pärn, K. Raidmets, T. Raidmets, K. Soans, T. Vellama

  14. Evaluation of the uranium anomaly of Punta del Este in the territory Isla de la Juventud

    International Nuclear Information System (INIS)

    Sanchez R, J.; Macola, E.; Alcalde, J.; Olivera, J.; Fernandez, P.; Villarino, J.

    1995-01-01

    The work show the projection and execution of the geologic-geophysics works on profiles executed to verificate the airborne spectrometric anomaly uranium in the territory the Island Young located in Punta del Este. As soon as the interpretation of the prospection works realizated as fundamental conclusion we obtained that this anomaly is associated to reed soils development over limestone, which have not contains of phosphorus

  15. Divina scriptura mundus est intelligibilis. L’ermeneutica biblica nel pensiero di Giovanni Scoto Eriugena

    OpenAIRE

    Cavallini, Andrea

    2012-01-01

    2010 - 2011 La tesi dottorale di Andrea Cavallini, dal titolo ‘Divina Scriptura mundus est intelligibilis’. L’ermeneutica biblica nel pensiero di Giovanni Scoto Eriugena, prende le mosse dalla constatazione che, nell’ormai vasto panorama della bibliografia eriugeniana, non esistono monografie che esaminino Giovanni Scoto nella sua attività di interprete della Bibbia. L’opera eriugeniana conta solo due scritti che si possono definire ‘esegetici’ in senso tecnico: un’omelia sul p...

  16. A Experiência Estética na Perspectiva Naturalista

    Directory of Open Access Journals (Sweden)

    Alberto Marinho Ribas Semeler

    2017-02-01

    Full Text Available Este artigo analisa algumas questões da neuroestética e suas relações com as reflexões históricas sobre a base biológica da experiência estética. A constatação dos vínculos entre biologia e estética não é novidade do mundo tecnológico, pois os gregos já pensavam a esse respeito, como é o caso da teoria naturalista de Aristóteles. O sentido da visão é compreendido enquanto meio produtor de conhecimento e intelecto visual. A neuroestética estabelece um diálogo entre tradição e inovação ao compreender as artes visuais como um subproduto da função evolutiva cerebral revalorizando-a no contexto da pesquisa científica.

  17. El argumento de la pobreza del estímulo, una vez más

    Directory of Open Access Journals (Sweden)

    Liza Skidelsky

    2016-11-01

    Full Text Available El argumento más conocido en favor del innatismo de ciertas estructuras mentales sigue siendo el ‘Argumento de la Pobreza del estímulo’ (APE. La idea general del APE es que el conocimiento que se requiere para desarrollar una cierta capacidad cognitiva excede en gran medida la información disponible en el entorno, de manera que el organismo contribuye con información innata. Un examen de la literatura del APE lingüístico muestra que aún no está del todo claro qué clase de argumento es y lo que realmente muestra. Mi objetivo en este trabajo es ofrecer un diagnóstico de la estrategia innatista que utiliza el APE. Así, distingo tres tipos de APE y argumento, en primer lugar, que la versión más apropiada, según ciertos criterios empíricos y teóricos, no parece ser suficiente para el innatismo lingüístico y, en segundo lugar, que para ser suficiente, suele complementarse con un argumento ‘de sillón’, cuya consecuencia es que convierte al innatismo en una hipótesis empírica debilitada.

  18. Characterization of variable EST SSR markers for Norway spruce (Picea abies L.

    Directory of Open Access Journals (Sweden)

    Spiess Nadine

    2011-10-01

    Full Text Available Abstract Background Norway spruce is widely distributed across Europe and the predominant tree of the Alpine region. Fast growth and the fact that timber can be harvested cost-effectively in relatively young populations define its status as one of the economically most important tree species of Northern Europe. In this study, EST derived simple sequence repeat (SSR markers were developed for the assessment of putative functional diversity in Austrian Norway spruce stands. Results SSR sequences were identified by analyzing 14,022 publicly available EST sequences. Tri-nucleotide repeat motifs were most abundant in the data set followed by penta- and hexa-nucleotide repeats. Specific primer pairs were designed for sixty loci. Among these, 27 displayed polymorphism in a testing population of 16 P. abies individuals sampled across Austria and in an additional screening population of 96 P. abies individuals from two geographically distinct Austrian populations. Allele numbers per locus ranged from two to 17 with observed heterozygosity ranging from 0.075 to 0.99. Conclusions We have characterized variable EST SSR markers for Norway spruce detected in expressed genes. Due to their moderate to high degree of variability in the two tested screening populations, these newly developed SSR markers are well suited for the analysis of stress related functional variation present in Norway spruce populations.

  19. La resistencia y la estética de la existencia en Michel Foucault

    Directory of Open Access Journals (Sweden)

    Reinaldo Giraldo Díaz

    2008-01-01

    parte del artículo me ocupo de las motivaciones del pensador francés para su retorno a Grecia; analizo los desplazamientos que realiza a lo largo de su tarea de búsqueda y la distinción que hace entre ética y moral. Igualmente, afirmo que Michel Foucault llega hasta la cultura griega y grecolatina y encuentra las técnicas de sí mismo que permiten la realización de una historia de la conformación de la subjetividad, a las cuales les da el nombre de artes de la existencia. En la segunda parte abordo la cuestión del poder pastoral, la gubernamentalidad y la Ilustración. Por último, teniendo en cuenta que Foucault no cree que el único punto posible de resistencia al poder político esté en la relación de uno consigo mismo, esta resistencia permite fragmentar el poder e inventar posibilidades de vida inéditas, pues, la tarea fundamental de nuestro tiempo consiste en realizar aquel tipo de acción que tiene como fin producir un nuevo tipo de subjetividad que permita liberarse a la vez del Estado y del tipo de individualización que está ligado a él, por lo cual, debemos promover nuevas formas de subjetividad por medio del rechazo de este tipo de individualidad que se nos ha impuesto durante siglos.

  20. O pensamento social de universitários sobre beleza e cirurgia estética

    Directory of Open Access Journals (Sweden)

    Luana Michele da Silva Vilas Bôas

    2017-01-01

    Full Text Available El estudio tuvo como objetivo identificar las representaciones sociales de hombres y mujeres con diferentes antecedentes académicos, en cuanto a belleza y cirugía estética. Este estudio es una investigación descriptiva y comparativa, con trazabilidad transversal, con la participación de 120 estudiantes universitarios; Plan de investigación múltiple método diseñado por A.S. de Rosa a través de un cuestionario semiestructurado, auto aplicable, dividido en cinco partes; que envuelve análisis estadístico descriptivo, relacional y análisis textual de redes asociativas. Los resultados ponen de manifiesto diferencias significativas en las representaciones sociales de los grupos en relación con los objetos sociales, belleza y cirugía estética, en términos de contenidos, evaluaciones y dimensiones socio-psicológicas. La proximidad de individuos y grupos con el cuerpo confirma parcialmente las evidencias de variables influyentes en términos de género y formación académica de los participantes en las representaciones de los objetos encuestados.

  1. Formação docente: reflexões sobre o estágio curricular

    Directory of Open Access Journals (Sweden)

    Jeâni Kelle Landre Rosa

    2012-01-01

    Full Text Available O presente artigo apresenta reflexões e discussões sobre a importância e o papel do estágio curricular na formação prática de alunos do curso de licenciatura em Ciências Biológicas. A partir da análise do estágio curricular dos acadêmicos do 7º e 9º período, foram identificados os aspectos favoráveis e desfavoráveis e formuladas sugestões que visam melhorar a qualidade do mesmo. A pesquisa acerca da realidade e dos problemas do estágio supervisionado foi realizada por meio de análise documental (diário de campo e narrativas e questionários. Com auxílio da literatura especializada, conseguimos aprofundar a compreensão dos mecanismos de articulação entre teoria e prática no cotidiano escolar, o que poderá contribuir para a construção de um currículo mais coerente com a realidade da sala de aula da Educação Básica.

  2. Restos de vida en Bajo este sol tremendo de Carlos Busqued

    Directory of Open Access Journals (Sweden)

    Marcos Seifert

    2017-07-01

    Full Text Available Este trabajo se propone analizar cómo la novela de Carlos Busqued Bajo este sol tremendo (2009 se apoya en la representación de vidas desechadas, basura y todo tipo de restos para instalarse en una tensión entre orden y exceso. La narración plantea una lógica en la que la basura, en lugar de constituirse en un indicio que posibilite la reconstrucción del paisaje social, exhibe lo desbordante, lo inasimilable y los vacíos de sentido. En el mundo que nos presenta Busqued lo viviente se vuelve resto en la medida en que encarna una heterogeneidad irreductible a cualquier estructura social y productiva. This paper analyzes the way in which Carlos Busqued's novel Bajo este sol tremendo (2009 is based on the representation of discarded lives, garbage, and all kinds of remains and thus proposes a tension between order and excess. The narration raises a logic in which garbage, instead of constituting an indicator that allows for the reconstruction of the social landscape, exhibits the overflowing, the unassimilable, and the voids of meaning. In the world that Busqued presents, the living becomes a remainder because it embodies an irreducible heterogeneity with regards to any social and productive structure.

  3. Development and Characterization of 37 Novel EST-SSR Markers in Pisum sativum (Fabaceae

    Directory of Open Access Journals (Sweden)

    Xiaofeng Zhuang

    2013-01-01

    Full Text Available Premise of the study: Simple sequence repeat markers were developed based on expressed sequence tags (EST-SSR and screened for polymorphism among 23 Pisum sativum individuals to assist development and refinement of pea linkage maps. In particular, the SSR markers were developed to assist in mapping of white mold disease resistance quantitative trait loci. Methods and Results: Primer pairs were designed for 46 SSRs identified in EST contiguous sequences assembled from a 454 pyrosequenced transcriptome of the pea cultivar, ‘LIFTER’. Thirty-seven SSR markers amplified PCR products, of which 11 (30% SSR markers produced polymorphism in 23 individuals, including parents of recombinant inbred lines, with two to four alleles. The observed and expected heterozygosities ranged from 0 to 0.43 and from 0.31 to 0.83, respectively. Conclusions: These EST-SSR markers for pea will be useful for refinement of pea linkage maps, and will likely be useful for comparative mapping of pea and as tools for marker-based pea breeding.

  4. Leer lo estético desde lo político

    Directory of Open Access Journals (Sweden)

    Verónica Peñafiel

    2013-06-01

    Full Text Available En el Ecuador, y específicamente en la vida política del país, el teatro ha jugado un papel más importante del que se le ha reconocido. Pensar lo estético relacionado con lo político, puede ser una interesante clave de lectura para el teatro quiteño y, me arriesgo a afirmar, para el teatro a secas. Este análisis no se lo hará desde el teatro político, se pretende, más bien, analizar la dimensión política del mismo y de la relación entre teatro y política. Dicha dimensión política ha tomado diversas caras. Con los matices posibles, ha pasado de las salas a la calle, del costumbrismo a lo grotesco, de las compañías a los grupos. Comprender estos matices con la óptica de lo político puede dar pistas para articular una lógica estética del teatro quiteño

  5. Comparación entre varias poblaciones estándar para el ajuste por edades

    Directory of Open Access Journals (Sweden)

    Llorca Díaz Javier

    2000-01-01

    Full Text Available FUNDAMENTO: Analizar el efecto que tiene la elección de una población de referencia sobre diferentes indicadores derivados de las tasas de mortalidad ajustadas por edad. MÉTODOS: Las tasas de mortalidad por diferentes causas de muerte en España de 1971 a 1992 son ajustadas empleando cuatro poblaciones de referencia: poblaciones españolas de 1971 y 1992, población estándar europea y población estándar mundial. Los resultados obtenidos con las cuatro poblaciones se comparan empleando tres indicadores: diferencia entre las tasas de 1992 y 1971, razón entre las tasas de 1992 y 1971y cambio anual porcentual entre 1971 y 1992. RESULTADOS: En la mayor parte de las causas de muerte estudiadas incluyendo el total de causas, la cardiopatía isquémica y casi todos los tumores, la razón de tasas y el porcentaje de cambio anual son similares con independencia de cuál sea la población estándar empleada. En cambio, la diferencia de tasas es muy diferente en función de la población de referencia. En las enfermedades infecciosas y el cáncer de testículo se produce la situación contraria: la diferencia de tasas es robusta mientras que la razón de tasas varía con la población estándar. Finalmente, la mortalidad por neumonía, enfermedad de Parkinson y cáncer de encéfalo muestra cambios en los tres indicadores utilizados. CONCLUSIONES: Es necesario realizar un análisis de las tasas específicas por edad antes de proceder a su ajuste. Este análisis permite averiguar si el ajuste por edades es correcto y qué indicador (diferencia, razón o cambio porcentual será adecuado para realizar comparaciones.

  6. Estética en prótesis removibles The aesthetics in removable prostheses

    Directory of Open Access Journals (Sweden)

    LVM Lucas

    2010-06-01

    Full Text Available La estética actualmente recibe una importante consideración en el contexto social. Una sonrisa atractiva es aspiración de la mayoría de los pacientes que precisan tratamiento protésico. Los implantes propiciaron una mejoría significativa, con relación a las prótesis removibles, debido a su funcionalidad. El objetivo de este estudio fue explorar en la literatura científica moderna, a fin de hallar aspectos relacionados con la estética en la rehabilitación mediante prótesis removibles. Fueron encontrados factores importantes como: la selección de los dientes artificiales, la disposición de los mismos, la caracterización de la base de resina acrílica, además de los tipos de retenedores para las prótesis parciales removibles. La planificación y aplicación apropiada de este procedimiento, con criterios estéticos correctos, debe ser responsabilidad del profesional.Nowadays, aesthetics is very appreciated in the social context and a attractive smile is the object of desire in most of patients requiring prosthesis treatment. In case of removable prosthesis, total or partial, this feature is more significant since some or all teeth may be absents. Implants leading to a significant improvement in relation to function and comfort of removable prosthesis users, but the aesthetics of these prostheses had little attention in the current scientific literature. Thus, the aim of present article was to make a review of the literature on the aesthetic features related to rehabilitation with removable prostheses. In present study we found as significant factors for the aesthetics in removable partial and total prostheses: selection of artificial teeth and their arrangement, characterization of acrylic resin basis and also the types and location of retainers for the removable partial prostheses. We conclude that the involvement and the aesthetic knowledge during the appropriate planning of this process is responsibility of the professional.

  7. Literatura chilena en digital: mapas, estéticas y conceptualizaciones

    Directory of Open Access Journals (Sweden)

    Carolina Gaínza C.

    2017-01-01

    Full Text Available En el presente artículo se busca analizar una experiencia estética vinculada a lo digital, que tiene como principales componentes la posibilidad de interactuar, participar y manipular las obras creadas en este formato. Con tal fin, se presentará un mapa de la literatura digital en Chile, para luego profundizar en el análisis de dos aspectos que permiten caracterizar una estética de lo digital: la hipertextualidad y el hackeo cultural. A partir de estos elementos y tomando en cuenta que la literatura digital es aquella creada para ser leída en la pantalla de un dispositivo electrónico, se analizan el poema A veces cubierto por las aguas de Carlos Cociña y el poemario Clickable poem@s de Luis Correa-Díaz. Estas obras nos permiten preguntarnos por el estatus de la literatura y la poesía en la era digital, vinculadas a una experiencia estética que pone énfasis en la intervención y el deseo de participación. PALABRAS CLAVE: literatura digital en Chile, poesía digital, estética digital, hipertexto. This article examines an aesthetic experience associated with the digital, which is characterized by the ability of the reader to interact, participate, and manipulate literary works created in this format. First, a map of Chilean digital literature will be presented and then two aspects will be analyzed which allow a description of an aesthetic of the digital: hypertextuality and cultural hacking. As a result of this analysis, and considering that digital literature is that which is created to be read on the screen of an electronic device, two poems will be investigated: A veces cubierto por las aguas by Carlos Cociña and Clickable poem@s by Luis Correa-Díaz. These texts allow us to think about the status of literature and poetry in the digital era, linked with an aesthetic experience which emphasizes intervention and the wish to participate. Keywords: Digital literature in Chile, digital poetry, digital aesthetics, hypertext.

  8. Gingivectomia como alternativa estética de la cirugía Periodontal

    Directory of Open Access Journals (Sweden)

    Stephanye Ariza Martínez

    2013-10-01

    Full Text Available Title: Gingivectomy like a cosmetic alternative of periodontal surgery.ResumenLa gingivectomía consiste en la escisión y eliminación de tejido gingival, con el objetivo del corte de la pared de tejidos blandos de una bolsa para disminuir su profundidad. Corrige dicha discrepancia, y elimina también bolsas supra óseas, agrandamientos fibrosos o edematosos de la encía, transformación de márgenes redondeados o engrosados en la forma ideal, y abscesos periodontales supra óseos. Para este procedimiento quirúrgico es necesario que el paciente tenga una encía insertada amplia, y dejar una zona funcionalmente adecuada para no eliminar la encía remanente y así no correr el riesgo de eliminar toda la encía. Se reporta un caso clínico de un paciente femenino de 28 años de edad que presenta inconformidad estética por dejar apreciar gran cantidad de encía cuando sonríe y por consiguiente dientes con corona clínica corta. Se obtuvieron excelentes resultados estéticos en el tratamiento y en su evolución. La finalidad de este reporte es presentar la gingivectomía como tratamiento estético periodontal en pacientes con sonrisa de encía que pueden ser antiestéticas para el paciente y conseguir una sonrisa más bella y armoniosa. (DUAZARY 2012 No. 1, 72 - 74AbstractGingivectomy involves the excision and removal of gum tissue, which aims to cut the soft tissue wall of a periodontal pocket and thus decrease its depth, corrects this discrepancy and also eliminates supra osseous pockets, eliminating gingival enlargements of gum process, conducing to margins rounded or thickened in the ideal, periodontal supra osseous. For this surgical procedure is necessary that the patient has a good quantity of attached gingival and leave an area not functionally adequate to remove the gum residue and thus avoid the risk of eliminating all the remaining gums. In this paper is reported a case of a female patient aged 28 presents aesthetic disagreement lot of

  9. La gratuité des soins est rentable : la preuve par un projet pilote au Burkina Faso

    OpenAIRE

    Blanchet, Karl; Zonon, Noël Adannou; Aggagliate, Thierry

    2012-01-01

    Karl Blanchet est chercheur à la London School of Hygiene and Tropical Medicine. Noël Adannou Zonon est expert en santé publique pour Terre des Hommes et Thierry Aggagliate est le directeur de Programmes de Terre des Hommes au Burkina Faso. Cet article est un résumé du document de capitalisation : K. Blanchet, N. Adannou Zonon, B. Nébié, T. Agagliate, G. Viala, Exemption de paiement : pour l’accès universel aux soins de santé des enfants de moins de 5 ans et des femmes enceintes. Bonnes prati...

  10. Pattern analysis approach reveals restriction enzyme cutting abnormalities and other cDNA library construction artifacts using raw EST data.

    Science.gov (United States)

    Zhou, Sun; Ji, Guoli; Liu, Xiaolin; Li, Pei; Moler, James; Karro, John E; Liang, Chun

    2012-05-03

    Expressed Sequence Tag (EST) sequences are widely used in applications such as genome annotation, gene discovery and gene expression studies. However, some of GenBank dbEST sequences have proven to be "unclean". Identification of cDNA termini/ends and their structures in raw ESTs not only facilitates data quality control and accurate delineation of transcription ends, but also furthers our understanding of the potential sources of data abnormalities/errors present in the wet-lab procedures for cDNA library construction. After analyzing a total of 309,976 raw Pinus taeda ESTs, we uncovered many distinct variations of cDNA termini, some of which prove to be good indicators of wet-lab artifacts, and characterized each raw EST by its cDNA terminus structure patterns. In contrast to the expected patterns, many ESTs displayed complex and/or abnormal patterns that represent potential wet-lab errors such as: a failure of one or both of the restriction enzymes to cut the plasmid vector; a failure of the restriction enzymes to cut the vector at the correct positions; the insertion of two cDNA inserts into a single vector; the insertion of multiple and/or concatenated adapters/linkers; the presence of 3'-end terminal structures in designated 5'-end sequences or vice versa; and so on. With a close examination of these artifacts, many problematic ESTs that have been deposited into public databases by conventional bioinformatics pipelines or tools could be cleaned or filtered by our methodology. We developed a software tool for Abnormality Filtering and Sequence Trimming for ESTs (AFST, http://code.google.com/p/afst/) using a pattern analysis approach. To compare AFST with other pipelines that submitted ESTs into dbEST, we reprocessed 230,783 Pinus taeda and 38,709 Arachis hypogaea GenBank ESTs. We found 7.4% of Pinus taeda and 29.2% of Arachis hypogaea GenBank ESTs are "unclean" or abnormal, all of which could be cleaned or filtered by AFST. cDNA terminal pattern analysis, as

  11. Pattern analysis approach reveals restriction enzyme cutting abnormalities and other cDNA library construction artifacts using raw EST data

    Directory of Open Access Journals (Sweden)

    Zhou Sun

    2012-05-01

    Full Text Available Abstract Background Expressed Sequence Tag (EST sequences are widely used in applications such as genome annotation, gene discovery and gene expression studies. However, some of GenBank dbEST sequences have proven to be “unclean”. Identification of cDNA termini/ends and their structures in raw ESTs not only facilitates data quality control and accurate delineation of transcription ends, but also furthers our understanding of the potential sources of data abnormalities/errors present in the wet-lab procedures for cDNA library construction. Results After analyzing a total of 309,976 raw Pinus taeda ESTs, we uncovered many distinct variations of cDNA termini, some of which prove to be good indicators of wet-lab artifacts, and characterized each raw EST by its cDNA terminus structure patterns. In contrast to the expected patterns, many ESTs displayed complex and/or abnormal patterns that represent potential wet-lab errors such as: a failure of one or both of the restriction enzymes to cut the plasmid vector; a failure of the restriction enzymes to cut the vector at the correct positions; the insertion of two cDNA inserts into a single vector; the insertion of multiple and/or concatenated adapters/linkers; the presence of 3′-end terminal structures in designated 5′-end sequences or vice versa; and so on. With a close examination of these artifacts, many problematic ESTs that have been deposited into public databases by conventional bioinformatics pipelines or tools could be cleaned or filtered by our methodology. We developed a software tool for Abnormality Filtering and Sequence Trimming for ESTs (AFST, http://code.google.com/p/afst/ using a pattern analysis approach. To compare AFST with other pipelines that submitted ESTs into dbEST, we reprocessed 230,783 Pinus taeda and 38,709 Arachis hypogaea GenBank ESTs. We found 7.4% of Pinus taeda and 29.2% of Arachis hypogaea GenBank ESTs are “unclean” or abnormal, all of which could be cleaned

  12. Rôle des protéines matricielles dans l’hypoxie et l’angiogenèse

    OpenAIRE

    Germain, Stéphane

    2016-01-01

        Responsable : Stéphane Germain Recherche : hypoxie, angiogenèse et intégrité vasculaire en pathologie cardiovasculaire et tumorale L’hypoxie, ou diminution de la pression partielle d’oxygène, est une caractéristique des tumeurs et des maladies cardiovasculaires ischémiques. Cette altération du microenvironnement hypoxique provoque une reprogrammation de l’expression de gènes codant pour des protéines impliquées dans la régulation de l’intégrité vasculaire et la reperfusion tissulaire. Not...

  13. Trabalho e conhecimento estético Work and aesthetical knowledge

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    Ronaldo Rosas Reis

    2004-09-01

    Full Text Available Este ensaio busca expor o conceito 'corpo trabalhador' e desenvolver uma reflexão crítica sobre o princípio educativo da estética e a origem do conhecimento artístico apreendido na teoria marxista como forma de contribuição para os estudos desenvolvidos na área de Trabalho e Educação, na qual identificamos uma lacuna quanto à questão estética como elemento de problematização da história cultural do trabalhador. A partir desta análise, o texto apresenta as principais demandas ideológicas e metamorfoses conceituais da teleologia burguesa da arte e do ensino de arte no Brasil nos documentos oficiais da legislação educacional brasileira mais recente.This essay attempts to examine the concept 'working body' and develop a critical reflection about the educational principle of aesthetics and the origin of artistic knowledge, understood in Marxist theory as a contribution to the studies developed in the areas of Work and Education. In our view, these studies fail to consider the aesthetical issue as one of the elements in the cultural history of the worker. On the basis of this analysis, the text presents the main ideological demands and conceptual changes of the bourgeois teleology of art and of the teaching of art in Brazil in the official documents of the most recent Brazilian legislation on education.

  14. Exercer a Postura Crítica: Desafios no Estágio em Psicologia Escolar

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    Adriana Marcondes Machado

    Full Text Available Os estudantes de graduação do curso de Psicologia do Instituto de Psicologia da Universidade de São Paulo, ao cursarem as disciplinas relacionadas à área da Psicologia Escolar, realizam estágios em instituições educativas. Imbuídos de uma postura crítica, os estagiários entram em contato com demandas de tratamento, prevenção e adaptação e compreendem a produção histórica destas. Todavia, em muitos momentos, os estagiários julgam essas demandas como se fossem equivocadas e, dessa forma, não conseguem perceber a multiplicidade de elementos que as constituem e as formas para agir em sua produção. O objetivo deste artigo é apresentar um percurso de trabalho enfatizando as estratégias criadas para dar conta de um dos desafios que se apresentam no grupo de supervisão: intervir nesse momento no qual os estagiários consideram o funcionamento da escola como algo exterior a eles. As estratégias criadas indicam que a desconstrução necessária na formação dos estudantes de Psicologia para que aprendam a intervir nos processos institucionais vividos durante os estágios requer, também, a desconstrução de uma forma de pensar que cria um suposto sujeito fora do diagrama de forças em que o processo do estágio faz parte.

  15. Characterization of simple sequence repeats (SSRs from Phlebotomus papatasi (Diptera: Psychodidae expressed sequence tags (ESTs

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    Hamarsheh Omar

    2011-09-01

    Full Text Available Abstract Background Phlebotomus papatasi is a natural vector of Leishmania major, which causes cutaneous leishmaniasis in many countries. Simple sequence repeats (SSRs, or microsatellites, are common in eukaryotic genomes and are short, repeated nucleotide sequence elements arrayed in tandem and flanked by non-repetitive regions. The enrichment methods used previously for finding new microsatellite loci in sand flies remain laborious and time consuming; in silico mining, which includes retrieval and screening of microsatellites from large amounts of sequence data from sequence data bases using microsatellite search tools can yield many new candidate markers. Results Simple sequence repeats (SSRs were characterized in P. papatasi expressed sequence tags (ESTs derived from a public database, National Center for Biotechnology Information (NCBI. A total of 42,784 sequences were mined, and 1,499 SSRs were identified with a frequency of 3.5% and an average density of 15.55 kb per SSR. Dinucleotide motifs were the most common SSRs, accounting for 67% followed by tri-, tetra-, and penta-nucleotide repeats, accounting for 31.1%, 1.5%, and 0.1%, respectively. The length of microsatellites varied from 5 to 16 repeats. Dinucleotide types; AG and CT have the highest frequency. Dinucleotide SSR-ESTs are relatively biased toward an excess of (AXn repeats and a low GC base content. Forty primer pairs were designed based on motif lengths for further experimental validation. Conclusion The first large-scale survey of SSRs derived from P. papatasi is presented; dinucleotide SSRs identified are more frequent than other types. EST data mining is an effective strategy to identify functional microsatellites in P. papatasi.

  16. Global comparative analysis of ESTs from the southern cattle tick, Rhipicephalus (Boophilus microplus

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    Pertea Geo

    2007-10-01

    Full Text Available Abstract Background The southern cattle tick, Rhipicephalus (Boophilus microplus, is an economically important parasite of cattle and can transmit several pathogenic microorganisms to its cattle host during the feeding process. Understanding the biology and genomics of R. microplus is critical to developing novel methods for controlling these ticks. Results We present a global comparative genomic analysis of a gene index of R. microplus comprised of 13,643 unique transcripts assembled from 42,512 expressed sequence tags (ESTs, a significant fraction of the complement of R. microplus genes. The source material for these ESTs consisted of polyA RNA from various tissues, lifestages, and strains of R. microplus, including larvae exposed to heat, cold, host odor, and acaricide. Functional annotation using RPS-Blast analysis identified conserved protein domains in the conceptually translated gene index and assigned GO terms to those database transcripts which had informative BlastX hits. Blast Score Ratio and SimiTri analysis compared the conceptual transcriptome of the R. microplus database to other eukaryotic proteomes and EST databases, including those from 3 ticks. The most abundant protein domains in BmiGI were also analyzed by SimiTri methodology. Conclusion These results indicate that a large fraction of BmiGI entries have no homologs in other sequenced genomes. Analysis with the PartiGene annotation pipeline showed 64% of the members of BmiGI could not be assigned GO annotation, thus minimal information is available about a significant fraction of the tick genome. This highlights the important insights in tick biology which are likely to result from a tick genome sequencing project. Global comparative analysis identified some tick genes with unexpected phylogenetic relationships which detailed analysis attributed to gene losses in some members of the animal kingdom. Some tick genes were identified which had close orthologues to mammalian genes

  17. Introduction : où en est l'analyse de réseaux en histoire?

    Directory of Open Access Journals (Sweden)

    Michel Bertrand

    2011-12-01

    Full Text Available Le concept de réseau est aujourd'hui largement entré dans le vocabulaire des sciences sociales. En histoire, l'introduction du vocabulaire des réseaux a souvent été liée à des démarches situées à une échelle « micro » et travaillant à mettre en évidence l'agency individuelle. Depuis les années 1990, une analyse de réseaux plus formalisée a fait des apparitions épisodiques, et inégales selon les domaines linguistiques, dans d'autres travaux historiques fondés au contraire sur des observations systématiques à une échelle macro. Après 30 ans d'une intégration de la catégorie à la démarche historique, un véritable savoir-faire historien émerge autour des questionnements auxquels elle est associée et des méthodologies qu'elle implique. Cependant, si l'analyse de réseaux a déjà largement fait la preuve de son intérêt dans certains domaines spécifiques de l’histoire, il existe trop peu de dialogue entre ceux qui la pratiquent ; notre souhait, en donnant à voir les parentés et les différences entre des textes issus de pays et de sous-disciplines variés, est bien de promouvoir un tel dialogue.

  18. Capacidad de carga estática en rodamientos. Normalización y tendencias.

    Directory of Open Access Journals (Sweden)

    A. García Toll

    2007-01-01

    Full Text Available Para ilustrar la funcionalidad de la norma NC-ISO 76: 2006, establecida a partir de una adopción idéntica de la Norma ISO 76: 1987/ amd.1: 1999 (E “Cojinetes de Rodamiento – Capacidad de Carga Estática”, son mostradas las correspondencias entre las magnitudes de capacidad de carga estática declaradas en los catálogos técnicos de reconocidos fabricantes de rodamientos con los valores presentes en NC-ISO 76 y considerando diferentes geometrías de rodamientos radiales de bolas rígidos y de contacto angular, rodamientos axiales de bolas y rodamientos de rodillos cilíndricos. Adicionalmente, el estudio ha permitido establecer las dependencias entre la capacidad de carga nominal estática del rodamiento y algunos parámetros geométricos, como el diámetro del elemento rodante, el diámetro interior y la relación diámetro interior - diámetro exterior del cojinete.At the present time, Standard NC-ISO 76: 2006 is a national standard established as identical adoption of ISO standard 76:1987 / amd.1: 1999 (E "Rolling Bearings - Static Load Rating¨. In this sense, the static load rating of rolling bearings declared in technical catalogs of renowned bearing manufacturers and the basic static load rating according to calculation procedures stated in Standard NC-ISO 76 are compared. The evaluation has taken into account different geometries of radial groove ball bearings, angular contact ball bearings, thrust ball bearings and radial roller bearings. Additionally, they are established dependences between the static load rating of rolling bearings and the interior diameter of different types of radial and thrust bearings.

  19. Horizonte negativo da arte na era da saturação estética

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    Eugênio Trivinho

    2001-11-01

    Full Text Available Inspirado no arco teórico que vai da Escola de Frankfurt ao pós-estruturalismo francês e ao pós-modernismo teórico, o presente ensaio discute a condição da arte no contexto social-histórico de excesso de produção tecnoestética, a partir da segunda metade do século XX. Na esteira (contra metodológica de radicalização de todas as hipóteses de trabalho, como política de reflexão teórica voltada para a otimização da epistème, a argumentação traça os principais momentos da trajetória social-histórica da arte. Nesse quadro, a tendência da argumentação, no que toca à empiria estética, descarta tanto o apoio melancólico a formas, esquemas e contextos artísticos do passado, quanto sobretudo o apoio político-ufanista a formas, esquemas e contextos artísticos que reforçam o fetichismo estético integral, socialmente instituído; e, no que toca à teoria, evita tanto o essencialismo da estética moderna de Theodor Adorno, quanto o niilismo irônico do pós-modernismo de Jean Baudrillard acerca do destino da arte.

  20. Determinación de sexo en restos prehistóricos aislados del este uruguayo

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    Figueiro, Gonzalo

    2001-01-01

    Full Text Available El frecuente hallazgo de restos humanos desarticulados y dispersos en sitios de la región Este del territorio uruguayo da lugar a la necesidad de recurrir en muchos casos a métodos alternativos de determinación de sexo, basados en análisis discriminantes. Las muestras de las que se parte en estos análisis son ya de poblaciones actuales, ya de poblaciones prehistóricas distintas a las representadas en el registro arqueológico uruguayo. Al determinar el número mínimo de individuos de un conjunto disperso, la edad y el sexo de los individuos representados cobran gran utilidad, y la precisión de los métodos de determinación de sexo basados en piezas aisladas se vuelve crucial. Este trabajo presenta la evaluación de 24 ecuaciones discriminantes canónicas sobre medidas de 7 huesos, en una muestra arqueológica de 15 individuos de dos sitios con estructuras monticulares ("cerritos de indios" del Este Uruguayo. El sexo de los esqueletos fue determinado anteriormente mediante métodos basados en la pelvis, el cráneo, o ambos. Los métodos evaluados presentaron grados de exactitud general de entre el 30 y el 100 por ciento, sin una dirección particular en los errores de clasificación. Esto pone de relieve la necesidad de revisar las ecuaciones utilizadas, recurriendo ya sea a una reelaboración de los coeficientes utilizados, o a un desplazamiento de los puntos de corte.

  1. A plausibilidade da pós-história no sentido estético

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    Rodrigo Duarte

    2011-01-01

    Full Text Available A ideia do "fim da história", subentendida no capítulo final da Fenomenologia do espírito, serviu de base para o início de uma discussão, feita a partir das posições assumidas por Alexandre Kojève nos seus cursos sobre Hegel em Paris, na década de 1930, e em sua publicação no final dos anos 1940 (com reedição em 1968, voltou à baila com o artigo de Francis Fukuyama, de 1989, sobre o "fim da história", no qual ele comemorava o fim do "socialismo real" e a hegemonia mundial completa dos Estados Unidos da América. Passada a euforia sobre a "nova ordem mundial", inclusive em virtude de sucessivas crises econômicas, é interessante recolocar a questão sobre as condições sob as quais são aceitáveis conceitos associados a esse tema, especialmente o substantivo "pós-história" e o adjetivo "pós-histórico". A tese a ser defendida nesse artigo é a de que o campo da estética é um âmbito em que esses conceitos são defensáveis. Como exemplos de reflexões estéticas frutíferas que deles se valem, são consideradas a noção de "arte pós-histórica", de Arthur Danto, e os desdobramentos estéticos do conceito de "pós-história", tal como sustentado por Vilém Flusser.

  2. MuSICa at GRIS: a prototype image slicer for EST at GREGOR

    Science.gov (United States)

    Calcines, A.; Collados, M.; López, R. L.

    2013-05-01

    This communication presents a prototype image slicer for the 4-m European Solar Telescope (EST) designed for the spectrograph of the 1.5-m GREGOR solar telescope (GRIS). The design of this integral field unit has been called MuSICa (Multi-Slit Image slicer based on collimator-Camera). It is a telecentric system developed specifically for the integral field, high resolution spectrograph of EST and presents multi-slit capability, reorganizing a bidimensional field of view of 80 arcsec^{2} into 8 slits, each one of them with 200 arcsec length × 0.05 arcsec width. It minimizes the number of optical components needed to fulfil this multi-slit capability, three arrays of mirrors: slicer, collimator and camera mirror arrays (the first one flat and the other two spherical). The symmetry of the layout makes it possible to overlap the pupil images associated to each part of the sliced entrance field of view. A mask with only one circular aperture is placed at the pupil position. This symmetric characteristic offers some advantages: facilitates the manufacturing process, the alignment and reduces the costs. In addition, it is compatible with two modes of operation: spectroscopic and spectro-polarimetric, offering a great versatility. The optical quality of the system is diffraction-limited. The prototype will improve the performances of GRIS at GREGOR and is part of the feasibility study of the integral field unit for the spectrographs of EST. Although MuSICa has been designed as a solar image slicer, its concept can also be applied to night-time astronomical instruments (Collados et al. 2010, Proc. SPIE, Vol. 7733, 77330H; Collados et al. 2012, AN, 333, 901; Calcines et al. 2010, Proc. SPIE, Vol. 7735, 77351X)

  3. The Macrostomum lignano EST database as a molecular resource for studying platyhelminth development and phylogeny.

    Science.gov (United States)

    Morris, Joshua; Ladurner, Peter; Rieger, Reinhard; Pfister, Daniela; Del Mar De Miguel-Bonet, Maria; Jacobs, David; Hartenstein, Volker

    2006-11-01

    We report the development of an Expressed Sequence Tag (EST) resource for the flatworm Macrostomum lignano. This taxon is of interest due to its basal placement within the flatworms. As such, it provides a useful comparative model for understanding the development of neural and sensory organization. It was anticipated on the basis of previous studies [e.g., Sánchez-Alvarado et al., Development, 129:5659-5665, (2002)] that a wide range of developmental markers would be expressed in later-stage macrostomids, and this proved to be the case, permitting recovery of a range of gene sequences important in development. To this end, an adult Macrostomum cDNA library was generated and 7,680 Macrostomum ESTs were sequenced from the 5' end. In addition, 1,536 of these aforementioned sequences were sequenced from the 3' end. Of the roughly 5,416 non-redundant sequences identified, 68% are similar to previously reported genes of known function. In addition, nearly 100 specific clones were obtained with potential neural and sensory function. From these data, an annotated searchable database of the Macrostomum EST collection has been made available on the web. A major objective was to obtain genes that would allow reconstruction of embryogenesis, and in particular neurogenesis, in a basal platyhelminth. The sequences recovered will serve as probes with which the origin and morphogenesis of lineages and tissues can be followed. To this end, we demonstrate a protocol for combined immunohistochemistry and in situ hybridization labeling in juvenile Macrostomum, employing homologs of lin11/lim1 and six3/optix. Expression of these genes is shown in the context of the neuropile/muscle system.

  4. Le criquet du Mato Grosso : l'agriculture est-elle responsable ?

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    Lecoq, M.

    1995-01-01

    Full Text Available Le criquet du Mato Grosso : l'agriculture est-elle responsable ?. Investigations through bibliographical references and among the local population provided a collection of documents and evidences showing without any doubt that Rhammatocerus schistocercoides (ReHN, 1906 [Orth. Acrididae Gomphocerinae] outbreaks in Mato Grosso (Brazil are not a new event neither by nature nor by magnitude. Therefore, the present conception on the origin of those outbreaks need to be drastically revised. The previous hypotheses, implying an overrated agricultural development of concerned areas since 1980, must then be rejected.

  5. Relatório de Estágio Curricular – Altran & Fidelidade Mundial (Lisboa)

    OpenAIRE

    Dias, Adriana

    2016-01-01

    O presente relatório descreve e ilustra o trabalho desenvolvido durante o estágio curricular do TeSP em Testes de Software através da Altran, na Fidelidade. Neste âmbito foram realizadas muitas atividades diversificadas, das quais se destacam os testes de software, (funcionais, exploratórios, regressivos e automáticos), a metodologia de gestão de atividades de teste, o desenvolvimento de manuais de suporte aos utilizadores das várias ferramentas utilizadas durante a execução dos testes de sof...

  6. L'IAGU est une ONG internationale spécialisée dans la recherche ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    DALBERG

    de l'IAGU sont pris en charge uniquement par les frais de gestion des projets de recherche. En 2008, la ... la préparation d'un Plan Local de Développement Territorial Durable, outil utilisable par les collectivités .... la stratégie et la définition d'un axe stratégique majeur qu'est le développement d'une offre diversifiée de ...

  7. Las notas graves de la sonata de los estímulos y la nota final

    Directory of Open Access Journals (Sweden)

    OSCAR COMAS RODRÍGUEZ

    2013-01-01

    Full Text Available Este ensayo plantea la necesidad de identificar los efectos del devenir, a lo largo de casi treinta años, de una política estatal en educación superior destinada a premiar económicamente los productos del trabajo académico. El cambio generacional, y las transformaciones del contexto, hacen ineludible proponer estrategias para el resurgimiento de la vida académica y hacer frente a las fisuras de metas y compromisos institucionales que se han visto alteradas, a partir de beneficios económicos no incorporados salarialmente a la normativa institucional.

  8. Cuerpos en Expresión: Vivencialidades, Prácticas, Estéticas

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    Adrián Scribano

    2016-04-01

    Full Text Available Con gran alegría compartmos con ustedes el número 20 de la Revista Latnoamericana de Estudios sobre Cuerpos, Emociones y Sociedad. Este número aniversario concreta un gran esfuerzo colectvo que desde el año 2009 y de manera cuatrimestral vienen realizando ininterrumpidamente el “Programa de Ac- ción Colectva y Conficto Social” del CIECS (CONICET y UNC, el “Grupo de Estudios sobre Sociología de las Emociones y los Cuerpos” del IIGG-UBA y la “Red La- tnoamericana de Estudios Sociales sobre las Emocio- nes y los Cuerpos”

  9. Estágio hospitalar em Optometria em Ciências da Visão

    OpenAIRE

    Macário, Daniel Fernando Bartolomeu

    2009-01-01

    Realizado o primeiro ano do Mestrado em Optometria em Ciências da Visão, onde varias vezes abordamos o paciente de optometria sob uma perspectiva diferente, surge então a oportunidade de realizar um estágio hospitalar de forma a estar um pouco mais dentro do dia-adia desse campo que varias vezes tinha ouvido falar mas que ainda me era desconhecido. Com a aprovação e o apoio do Dr. Santiago Garcia, médico oftalmologista do Hospital Universitário de Salamanca, tive a oportunidade de conhecer es...

  10. Medellín: ciudad ocasional: tres despliegues estéticos

    OpenAIRE

    Muñoz Castrillón, Sebastián

    2014-01-01

    La ciudad es un conjunto de afectaciones tangibles e intangibles que se configuran desde los espacios que son habitados por unas estructuras sociales en constante movimiento. Los espacios se encuentran cargados de particularidades que se reconocen en la sociedad a modo de sistemas autoorganizados. La confrontación de las formas de habitar el espacio en las ciudades marca diferencias y genera identidad a los territorios. En este caso se mira la ciudad de Medellín como un espacio en el cual la ...

  11. Costos estándar y su aplicación en el sector manufacturero colombiano

    OpenAIRE

    Duque-Roldán, María Isabel; Osorio-Agudelo, Jair Albeiro; Agudelo-Hernández, Didier Mauricio

    2011-01-01

    De acuerdo con José Álvarez-López, Joan Amat-i-Salas, Tomás Balada-Ortega, Felipe Blanco-Ibarra, Jesús Lizcano-Álvarez y Vicente Ripoll-Feliu (1996), el avance más notable que se ha producido en la Contabilidad de Costos con enfoque de gestión ha sido el desarrollo del Sistema de Costos Estándar. Su objetivo es disciplinar y controlar todas y cada una de las actividades y operaciones llevadas a cabo en la empresa con la finalidad de mejorar los resultados. Al prever y disciplinar los costos p...

  12. Estándares de interactividad en televisión híbrida

    Directory of Open Access Journals (Sweden)

    Rafael Sotelo

    2016-12-01

    Full Text Available La interactividad con contenido lineal como “a demanda” provisto por las emisoras de Televisión Digital terrestre es un requerimiento cada vez mayor por parte de los televidentes. Esta tecnología ha evolucionado hasta el concepto actual de Televisión Híbrida. El presente artículo describe la arquitectura general de los sistemas de Televisión Híbrida, y presenta los principales estándares de interactividad en Televisión Híbrida utilizados y en desarrollo actualmente a nivel mundial.

  13. Oil palm (Elaeis guineensis Jacq.) tissue culture ESTs: identifying genes associated with callogenesis and embryogenesis.

    Science.gov (United States)

    Low, Eng-Ti L; Alias, Halimah; Boon, Soo-Heong; Shariff, Elyana M; Tan, Chi-Yee A; Ooi, Leslie Cl; Cheah, Suan-Choo; Raha, Abdul-Rahim; Wan, Kiew-Lian; Singh, Rajinder

    2008-05-29

    Oil palm (Elaeis guineensis Jacq.) is one of the most important oil bearing crops in the world. However, genetic improvement of oil palm through conventional breeding is extremely slow and costly, as the breeding cycle can take up to 10 years. This has brought about interest in vegetative propagation of oil palm. Since the introduction of oil palm tissue culture in the 1970s, clonal propagation has proven to be useful, not only in producing uniform planting materials, but also in the development of the genetic engineering programme. Despite considerable progress in improving the tissue culture techniques, the callusing and embryogenesis rates from proliferating callus cultures remain very low. Thus, understanding the gene diversity and expression profiles in oil palm tissue culture is critical in increasing the efficiency of these processes. A total of 12 standard cDNA libraries, representing three main developmental stages in oil palm tissue culture, were generated in this study. Random sequencing of clones from these cDNA libraries generated 17,599 expressed sequence tags (ESTs). The ESTs were analysed, annotated and assembled to generate 9,584 putative unigenes distributed in 3,268 consensi and 6,316 singletons. These unigenes were assigned putative functions based on similarity and gene ontology annotations. Cluster analysis, which surveyed the relatedness of each library based on the abundance of ESTs in each consensus, revealed that lipid transfer proteins were highly expressed in embryogenic tissues. A glutathione S-transferase was found to be highly expressed in non-embryogenic callus. Further analysis of the unigenes identified 648 non-redundant simple sequence repeats and 211 putative full-length open reading frames. This study has provided an overview of genes expressed during oil palm tissue culture. Candidate genes with expression that are modulated during tissue culture were identified. However, in order to confirm whether these genes are suitable as

  14. Oil palm (Elaeis guineensis Jacq. tissue culture ESTs: Identifying genes associated with callogenesis and embryogenesis

    Directory of Open Access Journals (Sweden)

    Ooi Leslie CL

    2008-05-01

    Full Text Available Abstract Background Oil palm (Elaeis guineensis Jacq. is one of the most important oil bearing crops in the world. However, genetic improvement of oil palm through conventional breeding is extremely slow and costly, as the breeding cycle can take up to 10 years. This has brought about interest in vegetative propagation of oil palm. Since the introduction of oil palm tissue culture in the 1970s, clonal propagation has proven to be useful, not only in producing uniform planting materials, but also in the development of the genetic engineering programme. Despite considerable progress in improving the tissue culture techniques, the callusing and embryogenesis rates from proliferating callus cultures remain very low. Thus, understanding the gene diversity and expression profiles in oil palm tissue culture is critical in increasing the efficiency of these processes. Results A total of 12 standard cDNA libraries, representing three main developmental stages in oil palm tissue culture, were generated in this study. Random sequencing of clones from these cDNA libraries generated 17,599 expressed sequence tags (ESTs. The ESTs were analysed, annotated and assembled to generate 9,584 putative unigenes distributed in 3,268 consensi and 6,316 singletons. These unigenes were assigned putative functions based on similarity and gene ontology annotations. Cluster analysis, which surveyed the relatedness of each library based on the abundance of ESTs in each consensus, revealed that lipid transfer proteins were highly expressed in embryogenic tissues. A glutathione S-transferase was found to be highly expressed in non-embryogenic callus. Further analysis of the unigenes identified 648 non-redundant simple sequence repeats and 211 putative full-length open reading frames. Conclusion This study has provided an overview of genes expressed during oil palm tissue culture. Candidate genes with expression that are modulated during tissue culture were identified. However

  15. Transformation de la production avicole en Asie du Sud-Est | CRDI ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    4 mai 2016 ... Depuis l'épidémie de grippe aviaire qui a frappé la Chine et l'Asie du Sud-Est au début des années 2000, les politiques gouvernementales visant à réduire le risque de nouvelles flambées ont donné lieu au déménagement de milliers de petits aviculteurs dans des zones de production avicole désignées.

  16. Generation and analysis of 9792 EST sequences from cold acclimated oat, Avena sativa

    Science.gov (United States)

    Bräutigam, Marcus; Lindlöf, Angelica; Zakhrabekova, Shakhira; Gharti-Chhetri, Gokarna; Olsson, Björn; Olsson, Olof

    2005-01-01

    Background Oat is an important crop in North America and northern Europe. In Scandinavia, yields are limited by the fact that oat cannot be used as a winter crop. In order to develop such a crop, more knowledge about mechanisms of cold tolerance in oat is required. Results From an oat cDNA library 9792 single-pass EST sequences were obtained. The library was prepared from pooled RNA samples isolated from leaves of four-week old Avena sativa (oat) plants incubated at +4°C for 4, 8, 16 and 32 hours. Exclusion of sequences shorter than 100 bp resulted in 8508 high-quality ESTs with a mean length of 710.7 bp. Clustering and assembly identified a set of 2800 different transcripts denoted the Avena sativa cold induced UniGene set (AsCIUniGene set). Taking advantage of various tools and databases, putative functions were assigned to 1620 (58%) of these genes. Of the remaining 1180 unclassified sequences, 427 appeared to be oat-specific since they lacked any significant sequence similarity (Blast E values > 10-10) to any sequence available in the public databases. Of the 2800 UniGene sequences, 398 displayed significant homology (BlastX E values ≤ 10-10) to genes previously reported to be involved in cold stress related processes. 107 novel oat transcription factors were also identified, out of which 51 were similar to genes previously shown to be cold induced. The CBF transcription factors have a major role in regulating cold acclimation. Four oat CBF sequences were found, belonging to the monocot cluster of DREB family ERF/AP2 domain proteins. Finally in the total EST sequence data (5.3 Mbp) approximately 400 potential SSRs were found, a frequency similar to what has previously been identified in Arabidopsis ESTs. Conclusion The AsCIUniGene set will now be used to fabricate an oat biochip, to perform various expression studies with different oat cultivars incubated at varying temperatures, to generate molecular markers and provide tools for various genetic

  17. Háptica en el automóvil: Condiciones estéticas

    OpenAIRE

    Pozuelo Segura, Eduardo

    2009-01-01

    La palabra HÁPTICA proviene del griego “Hapthai” y se refiere a todo lo relativo al sentido del tacto. Sin embargo, las nuevas tecnologías se han encargado de ampliar el significado de la palabra a “todas las sensaciones que experimenta un individuo con sus cinco sentidos”. El presente proyecto analiza en detalle este nuevo concepto de háptica en el ámbito de la automoción. Ha sido realizado en las instalaciones del Centro Técnico de SEAT en Martorell (Barcelona), en la secc...

  18. Filosofía y literatura: la experiencia estética en Estanislao Zuleta

    OpenAIRE

    Perdigón Lesmes, Cristhian

    2013-01-01

    Dos son los principales propósitos de esta investigación: por un lado, dar cuenta de la manera en que Estanislao Zuleta hizo filosofía desde la literatura, y, por otro, destacar como consecuencia principal de un hacer filosófico tan particular la presencia en la obra de Zuleta de una inconclusa teoría estética. En procura de cumplir estos objetivos, tres serán los derroteros a seguir: primero, desde el concepto de Lebenswelt y la noción freudiana de sublimación, justificar la compleja relació...

  19. Relatório de estágio supervisionado (3º ciclo)

    OpenAIRE

    Campos, Paula Maria Fernandes

    2010-01-01

    A prática pedagógica é uma unidade curricular prevista no Mestrado em Ensino de Educação Musical do Ensino básico, figurando o estágio, naquela compreendido, como uma oportuniddade para fazer a conexão entre a prática e a teoria, de forma reflexiva, de toda a aprendizagem até então adquirida. Prevê, de igual modo, que se concretize o domínio das técnicas do ensino/aprendizagem, habilitando desse modo para o exercício da actividade profissional de professor. (...)

  20. Antimatière Est-elle la clé de l'Univers?

    CERN Multimedia

    Grousson, Mathieu

    2009-01-01

    "De quoi est fait l'Univers? De matière ordinaire et, selon la théorie actuelle, de matière noire et d'énergie noire... dont nul ne sait rien! Et s'il s'agissait plutôt d'antimatière? Car elle permet de réécrire l'histoire du cosmos sans déroger à ses lois et, cette fois, sans nulle zone d'ombre. Reste à vérifier qu'elle antigravite bien..." (14 pages)

  1. Madres adultas y madres adolescentes, ¿dónde están las diferencias?

    OpenAIRE

    Hidalgo García, María Victoria; Moreno Rodríguez, María del Carmen; Sánchez Hidalgo, José

    2002-01-01

    En este artículo presentamos los resultados de una investigación en la que se estudió el proceso de convertirse en madres de 107 mujeres, 48 madres adolescentes y 59 madres adultas. Los resultados de nuestro estudio han mostrado que la maternidad adolescente y la maternidad adulta son realidades diferentes. En concreto, las chicas que se convierten en madres durante la adolescencia cuentan con menos recursos y fuentes de apoyo para el ejercicio de su maternidad, mantienen ideas evolutivo-educ...

  2. Quid est veritas? Augustinus oor waarheid en leuen - en latere ontwikkelings1

    Directory of Open Access Journals (Sweden)

    J. H. van Wyk

    2000-12-01

    Full Text Available Quid est veritas? Augustine on truth and lying - and the aftermath. In this article the author investigates the (very difficult issue of truth and lying. What does it mean to speak the truth and when does a person tell a lie? The Augustinian as well as post-Augustinian views are enumerated and some of the main truth theories evaluated. In conclusion the author tries to make a final assessment and to open new ways of thinking the truth. Truth is a multidimensional category which should never be over-simplified.

  3. Geología del este de Costa Rica y el norte de Panamá

    OpenAIRE

    Escalante, Gregorio; Astorga, Allana

    2011-01-01

    Costa Rica y Panamá forman parte del Bloque Chorotega, el cual conforma una provincia geológica de basamento oceánico, de edad Mesozoica - Conozoica que representa un arco de islas intraoceánico maduro, que ha evolucionado desde el Cretácico Superior. El segmento formado por el este de Costa Rica y el norte de Panamá constituye una unidad tectónica limitada por fallas, donde el límite norte corresponde con el Cinturón Deformado del Norte de Panamá, que en la latitud de Puerto Limón, se transf...

  4. Comentario al margen del artículo estenosis malignas del estómago

    Directory of Open Access Journals (Sweden)

    Mario Gaitán Yanguas

    1954-09-01

    Sin embargo, existen en los archivos del Instituto varios casos de sarcomas gástricos que me parece deben hacerse conocer no con el ánimo de polemizar ni de restar meritos a tan valioso aporte científico como es el de Jácome y colaboradores, sino únicamente como contribución al conocimiento de un problema de tanta importancia como es el del cáncer del estómago y especialmente en lo que se refiere desde el punto de vista patológico a los sarcomas.

  5. Análisis retórico del esténcil o estarcido

    Directory of Open Access Journals (Sweden)

    Heiner Mercado-Percia

    2012-01-01

    Full Text Available En esta exposición intentaré dar respuesta a las siguientes preguntas: ¿Puede expresarse un discurso retórico por medio de una imagen? Y si esto es posible ¿cómo esta imagen puede persuadir a un auditorio? Estas preguntas surgen a partir de una imagen de esténcil o estarcido llamada Disney War,apareció en los muros de muchas ciudades del mundo, incluida Medellín, y que es promocionada o expuesta a través de la Internet.

  6. L'Inde : où est le milieu ? | IDRC - International Development ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    1 avr. 2011 ... Des entrepreneurs futés, favorisés par les réformes économiques opérées au début des années 1990, ont tiré parti des nouveaux débouchés sur les ... À long terme toutefois, M. Mazumdar est d'avis qu'il s'agit d'un encouragement manifeste à privilégier l'expansion horizontale plutôt que verticale. S'il était ...

  7. License - ClEST | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us ClEST License License to Use This Database Last updated : 2012/05/22 You may use this database...ense terms regarding the use of this database and the requirements you must follow in using this database. The license for this datab...re Alike 2.1 Japan . If you use data from this database, please be sure attribute this database as follows: ...tive Commons Attribution-Share Alike 2.1 Japan is found here . With regard to this database..., you are licensed to: freely access part or whole of this database, and acquire data; freely redi

  8. Uma nova proposta para o estágio de pratica de ensino em psicologia

    Directory of Open Access Journals (Sweden)

    Mary Neide Damico Figueiró

    1991-08-01

    Full Text Available The objective of this work was to propose a new alternative for the Teaching Practicum in Psychology which could provide better experiences for the licentiate student in the Educational field in terms of quality and in regards to the community needs. The methodology of this work consisted of planning, accomplishing and evaluating the extension courses and events of 20 or 30 hours taught by the licentiate students to Primary and Secondary school students, teachers of pre, school and primary school, nursery employees and the community in general. The results showed that in a significantly higher proportion the licentiate students considered, the experience in Teachining Practicum and the contribution of it to his/her professional career to be excellent. The clientele, as a whole, evaluated positively the quality of the courses and the range of the proposed objectives. It was concluded that the new alternative has brought new improvement to the Teaching Practicum, providing an integration of students of Higher Education with the primary and secondary teaching and the community in general" in favor of the knowledge democratization. O objetivo do presente trabalho foi propor uma nova alternativa para os estágios de Prática de Ensino em Psicologia que aprimorasse as experiências do licenciando no campo da Educação, dando mais qualidade a esses estágios, num vínculo com as necessidades da comunidade. A metodologia consistiu no planejamento, execução e avaliação de cursos-evento-extensão de 20 ou 30 horas pelos licenciados junto a alunos de 1° e 2° graus, professores de pré-escola e 1° grau, funcionários de creche e comunidade em geral. Quanto aos resultados, os licenciandos avaliaram, numa proporção significativamente maior, como sendo ótima a experiência em Prática de Ensino e a contribuição do estágio para a sua vida profissional. A clientela, em sua totalidade, avaliou de forma positiva a qualidade dos cursos e o alcance dos

  9. Práticas de letramento docente no estágio supervisionado de letras estrangeiras

    Directory of Open Access Journals (Sweden)

    Carla Lynn Reichmann

    Full Text Available Neste artigo discuto a relevância de práticas de letramento no estágio. Alinhando-me aos novos estudos de letramento (BARTON et al., 2000 e considerando a escrita como elemento identitário de formação (KLEIMAN, 2007, adoto a perspectiva bakhtiniana de gênero a fim de investigar relatos autobiográficos alicerçados em fotobiografias escolares produzidas em uma disciplina de estágio em língua estrangeira, em 2011. Neste recorte analiso as vozes enunciativas que ecoam em dois relatos, em especial as vozes de personagem e autor (BRONCKART, 1999; 2006, para sintonizar com as vozes e identidades sociais que se constituem nos textos. Em suma, retomando Kleiman (2006; 2011, saliento o impacto vital do trabalho do professor como agente de letramento, levando em conta a heterogeneidade das trajetórias de letramento e formação.

  10. « Un seul oiseau en cage / La liberté est en deuil »

    Directory of Open Access Journals (Sweden)

    Concours Twittérature service culturel de l’Ambassade de France au Canada

    2017-05-01

    Full Text Available « Un seul oiseau en cage / La liberté est en deuil » (Jacques Prévert Concours de twittérature (Semaine de la Francophonie 2017 Pour célébrer la Semaine de la Francophonie 2017, le service culturel de l’Ambassade de France au Canada a lancé en mars dernier un concours de twittérature, autour de la figure du poète et artiste français Jacques Prévert, disparu il y a tout juste quarante ans. En 140 signes maximum – incluant le mot dièse #Prévert et le tag @francetoronto – élèves, enseignants, parents, poètes en herbe comme poètes confirmés, ont pu laisser libre cours à leur imagination en répondant au vers de la plume de Prévert – « Un seul oiseau en cage / La liberté est en deuil » – à partir de leur compte Twitter.

  11. Overexpressed Genes/ESTs and Characterization of Distinct Amplicons on 17823 in Breast Cancer Cells

    Directory of Open Access Journals (Sweden)

    Ayse E. Erson

    2001-01-01

    Full Text Available 17823 is a frequent site of gene amplification in breast cancer. Several lines of evidence suggest the presence of multiple amplicons on 17823. To characterize distinct amplicons on 17823 and localize putative oncogenes, we screened genes and expressed sequence tags (ESTs in existing physical and radiation hybrid maps for amplification and overexpression in breast cancer cell lines by semiquantitative duplex PCR, semiquantitative duplex RT-PCR, Southern blot, Northern blot analyses. We identified two distinct amplicons on 17823, one including TBX2 and another proximal region including RPS6KB1 (PS6K and MUL. In addition to these previously reported overexpressed genes, we also identified amplification and overexpression of additional uncharacterized genes and ESTs, some of which suggest potential oncogenic activity. In conclusion, we have further defined two distinct regions of gene amplification and overexpression on 17823 with identification of new potential oncogene candidates. Based on the amplification and overexpression patterns of known and as of yet unrecognized genes on 17823, it is likely that some of these genes mapping to the discrete amplicons function as oncogenes and contribute to tumor progression in breast cancer cells.

  12. Sentido de la responsabilidad con la salud: perspectiva de sujetos que reivindican este derecho

    Directory of Open Access Journals (Sweden)

    Ángela María Franco-Cortés, Colombia.

    2015-07-01

    Full Text Available (analítico: En este artículo presentamos los resultados de una investigación que realizamos con el objetivo de comprender el sentido que tiene la responsabilidad con la salud para miembros de organizaciones sociales que se movilizan por el derecho a la vida saludable. Es una investigación cualitativa, soportada epistemológica y metodológicamente en la hermenéutica y en la fenomenología social. Develamos tres tendencias sobre el sentido de la responsabilidad con la salud que orientan las acciones individuales y colectivas de estos sujetos: la responsabilidad como compasión (perspectiva judeo-cristiana y como actitud de servicio con quien está enfermo; como tensión entre la presencia del Estado y la resistencia/movilización de los ciudadanos y las ciudadanas por el derecho a los servicios; y como compromiso político y vinculación solidaria a un proyecto colectivo por una sociedad distinta.

  13. Sentido de la responsabilidad con la salud: perspectiva de sujetos que reivindican este derecho

    Directory of Open Access Journals (Sweden)

    Ángela María Franco-Cortés

    2015-01-01

    Full Text Available En este artículo presentamos los resultados de una investigación querealizamos con el objetivo de comprender el sentido que tiene la responsabilidad con la salud paramiembros de organizaciones sociales que se movilizan por el derecho a la vida saludable. Es unainvestigación cualitativa, soportada epistemológica y metodológicamente en la hermenéutica y enla fenomenología social. Develamos tres tendencias sobre el sentido de la responsabilidad con lasalud que orientan las acciones individuales y colectivas de estos sujetos: la responsabilidad comocompasión (perspectiva judeo-cristiana y como actitud de servicio con quien está enfermo; comotensión entre la presencia del Estado y la resistencia/movilización de los ciudadanos y las ciudadanaspor el derecho a los servicios; y como compromiso político y vinculación solidaria a un proyectocolectivo por una sociedad distinta.

  14. El escritor no es nada, Nadie: Saer contra una estética latinoamericana

    Directory of Open Access Journals (Sweden)

    Rafael Arce

    2009-07-01

    Full Text Available En un ensayo estupendo, Alejo Carpentier señala la dificultad que entraña dar definiciones sobre nociones harto complejas que atraviesan la historia del arte y de la literatura. No obstante, su recorrido lo obliga a asumir cierta definición o, mejor, cierto rasgo conceptual que permitiría el acceso a una cierta definición, para el caso de su interés, del barroco, noción harto compleja que lo ayudará para llegar a la de lo real maravilloso. Después de una breve síntesis acerca de los avatares de esta noción a través de la historia del arte, ilustrada sobre todo por ejemplos tomados de la arquitectura, Carpentier se decide por este rasgo capital: lo que caracterizaría más propiamente al barroco como posición estética es un declarado horror al vacío. La caracterización, el rasgo conceptual, es de tipo causal: el horror al vacío sería la causa, el motivo, del movimiento, o de la actitud más bien, barroca.

  15. El escritor no es nada, Nadie: Saer contra una estética latinoamericana

    Directory of Open Access Journals (Sweden)

    Rafael Arce

    2009-06-01

    Full Text Available En un ensayo estupendo, Alejo Carpentier señala la dificultad que entraña dar definiciones sobre nociones harto complejas que atraviesan la historia del arte y de la literatura. No obstante, su recorrido lo obliga a asumir cierta definición o, mejor, cierto rasgo conceptual que permitiría el acceso a una cierta definición, para el caso de su interés, del barroco, noción harto compleja que lo ayudará para llegar a la de lo real maravilloso. Después de una breve síntesis acerca de los avatares de esta noción a través de la historia del arte, ilustrada sobre todo por ejemplos tomados de la arquitectura, Carpentier se decide por este rasgo capital: lo que caracterizaría más propiamente al barroco como posición estética es un declarado horror al vacío. La caracterización, el rasgo conceptual, es de tipo causal: el horror al vacío sería la causa, el motivo, del movimiento, o de la actitud más bien, barroca.

  16. Prerrafaelismo y quietismo estético en Aromas de leyenda

    Directory of Open Access Journals (Sweden)

    Sánchez Moreiras, Miriam

    2005-12-01

    Full Text Available In my Aromas de leyenda study, book of poems wroten by the Spanish Modernist author Valle- Inclán, I have attempted to show how this work is in debt to Pre-Raphaelite aesthetics and, also, to esoteric and mystic doctrines, strongly present in the last years of XIX century and relaborated by Valle in his book La lámpara maravillosa. At the same time, I have identified, although lightly, the figure oí ambivalence, which is, like the scholarship Peter Zima has showed, the main Modernism aesthetic innovation and which makes in clear the links between this Valle's work and the art of his time.Con el análisis de Aromas de leyenda de Valle Inclán he pretendido demostrar la deuda que esta obra tiene tanto con el código estético prerrafaelita como con las doctrinas esotéricas y místicas, de gran vigencia a finales del siglo XIX y reelaboradas por Valle en su libro de 1916 La lámpara maravillosa. Igualmente he identificado, aunque de modo muy leve, la presencia en Aromas de la figura de la ambivalencia, que es, como demuestra el estudioso Peter Zima, la principal innovación estética del Modernismo y que pone en evidencia la filiación de esta obra de Valle con el arte de su tiempo.

  17. SSR and EST-SSR-based genetic linkage map of cassava (Manihot esculenta Crantz).

    Science.gov (United States)

    Sraphet, Supajit; Boonchanawiwat, Athipong; Thanyasiriwat, Thanwanit; Boonseng, Opas; Tabata, Satoshi; Sasamoto, Shigemi; Shirasawa, Kenta; Isobe, Sachiko; Lightfoot, David A; Tangphatsornruang, Sithichoke; Triwitayakorn, Kanokporn

    2011-04-01

    Simple sequence repeat (SSR) markers provide a powerful tool for genetic linkage map construction that can be applied for identification of quantitative trait loci (QTL). In this study, a total of 640 new SSR markers were developed from an enriched genomic DNA library of the cassava variety 'Huay Bong 60' and 1,500 novel expressed sequence tag-simple sequence repeat (EST-SSR) loci were developed from the Genbank database. To construct a genetic linkage map of cassava, a 100 F(1) line mapping population was developed from the cross Huay Bong 60 by 'Hanatee'. Polymorphism screening between the parental lines revealed that 199 SSRs and 168 EST-SSRs were identified as novel polymorphic markers. Combining with previously developed SSRs, we report a linkage map consisted of 510 markers encompassing 1,420.3 cM, distributed on 23 linkage groups with a mean distance between markers of 4.54 cM. Comparison analysis of the SSR order on the cassava linkage map and the cassava genome sequences allowed us to locate 284 scaffolds on the genetic map. Although the number of linkage groups reported here revealed that this F(1) genetic linkage map is not yet a saturated map, it encompassed around 88% of the cassava genome indicating that the map was almost complete. Therefore, sufficient markers now exist to encompass most of the genomes and efficiently map traits in cassava.

  18. Factores asociados a recaídas por tuberculosis en Lima este - Perú

    Directory of Open Access Journals (Sweden)

    María Ríos Hipólito

    2002-01-01

    Full Text Available Objetivo: Determinar los factores de riesgo asociados a recaídas por tuberculosis en Lima Este - Perú, entre marzo y diciembre del 2000. Materiales y métodos: estudio caso-control. Se definió a los casos (184 como los pacientes que recibieron tratamiento con el esquema I alguna vez, egresaron como curados y volvieron a presentar otro episodio de tuberculosis BK positivo durante 1999. Los controles (368 fueron los pacientes nuevos con tuberculosis BK positivo tratados en 1998 que no recayeron. Resultados: se asociaron significativamente a las recaídas el sexo masculino, la edad mayor de 50 años, el consumo de drogas, la residencia en un área urbana, el hacinamiento, la percepción errada de la enfermedad (PEE y la desocupación, no así el contacto con un paciente tuberculoso. Luego del análisis multivariado, sólo se asociaron el área urbana, el hacinamiento, la PEE y el tratamiento irregular. Conclusiones: la residencia en un área urbana, el hacinamiento, la PEE y la irregularidad en el tratamiento son factores asociados significativamente a recaídas en pacientes con TBC pulmonar BK(+ de Lima Este, Perú.

  19. Nitrogen assimilation in Citrus based on CitEST data mining

    Directory of Open Access Journals (Sweden)

    Ester Wickert

    2007-01-01

    Full Text Available Assimilation of nitrate and ammonium are vital procedures for plant development and growth. From these primary paths of inorganic nitrogen assimilation, this metabolism integrates diverse paths for biosynthesis of macromolecules, such as amino acids and nucleotides, and the central intermediate metabolism, like carbon metabolism and photorespiration. This paper reports research performed in the CitEST (Citrus Expressed Sequence Tag database for the main genes involved in nitrogen metabolism and those previously described in other organisms. The results show that a complete cluster of genes involved in the assimilation of nitrogen and the metabolisms of glutamine, glutamate, aspartate and asparagine can be found in the CitEST data. The main enzymes found were nitrate reductase (NR, nitrite reductase (NiR, glutamine synthetase (GS, glutamate synthetase (GOGAT, glutamate dehydrogenase (GDH, aspartate aminotransferase (AspAT and asparagine synthetase (AS. The different enzymes involved in this metabolism have been shown to be highly conserved among the Citrus and Poncirus species. This work serves as a guide for future functional analysis of these enzymes in citrus.

  20. CAMÕES REVISITADO: (SOBRE QUE FAREI COM ESTE LIVRO?, DE JOSÉ SARAMAGO

    Directory of Open Access Journals (Sweden)

    Marcio Roberto Pereira

    2014-12-01

    Full Text Available

    O objetivo deste trabalho é analisar a construção literária de Camões na obra Que farei com este livro?, de José Saramago. Por causa da dificuldade em publicar sua obra máxima, Os Lusíadas, Camões percorre as diversas instâncias portuguesas no intuito de conseguir dar concretude ao seu trabalho de anos no exílio. Recorrendo a aspectos históricos e lacunas sobre a biografia de Camões, Saramago cria uma obra que mostra a inércia que reinava em Portugal após as grandes conquistas. Obra de contraponto entre o passado e o presente, Que farei com este livro? representa uma importante reflexão sobre o papel do escritor e seus desafios em momentos de decadência.

  1. Masse rénale avec image stellaire centrale: Quel est votre diagnostic?

    Directory of Open Access Journals (Sweden)

    A. Qarro

    2013-03-01

    Full Text Available Le carcinome chromophobe se voit essentiellement à la cinquième décennie et représente 5% des tumeurs rénales. Il existe deux sous types de cancer à cellules chromophobes: le type clair (70% et le type éosinophile. La variante à cellules éosinophiles doit être distinguée de l'oncocytome. Ce dernier se caractérise par une cicatrice fibreuse centrale d'aspect stellaire. Le carcinome chromophobe et l'oncocytome peuvent même coexister dans le cadre du syndrome de Birt-Hogg-Dubé. Certaines tumeurs appelées hybrides partagent des caractéristiques architecturales et cytologiques de ces deux tumeurs. Le pronostic du carcinome chromophobe est favorable. Il est le plus souvent limité au rein et de bas grade nucléaire. Il semblerait donc licite dans les cas ou un examen extemporané mettrait en évidence une tumeur chromophobe, de limiter l'intervention à une néphrectomie partielle si techniquement réalisable.

  2. Gene discovery using next-generation pyrosequencing to develop ESTs for Phalaenopsis orchids

    Science.gov (United States)

    2011-01-01

    Background Orchids are one of the most diversified angiosperms, but few genomic resources are available for these non-model plants. In addition to the ecological significance, Phalaenopsis has been considered as an economically important floriculture industry worldwide. We aimed to use massively parallel 454 pyrosequencing for a global characterization of the Phalaenopsis transcriptome. Results To maximize sequence diversity, we pooled RNA from 10 samples of different tissues, various developmental stages, and biotic- or abiotic-stressed plants. We obtained 206,960 expressed sequence tags (ESTs) with an average read length of 228 bp. These reads were assembled into 8,233 contigs and 34,630 singletons. The unigenes were searched against the NCBI non-redundant (NR) protein database. Based on sequence similarity with known proteins, these analyses identified 22,234 different genes (E-value cutoff, e-7). Assembled sequences were annotated with Gene Ontology, Gene Family and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Among these annotations, over 780 unigenes encoding putative transcription factors were identified. Conclusion Pyrosequencing was effective in identifying a large set of unigenes from Phalaenopsis. The informative EST dataset we developed constitutes a much-needed resource for discovery of genes involved in various biological processes in Phalaenopsis and other orchid species. These transcribed sequences will narrow the gap between study of model organisms with many genomic resources and species that are important for ecological and evolutionary studies. PMID:21749684

  3. Câncer de estômago: fatores de risco

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    Anna Valéria de Britto

    Full Text Available Há cinqüenta anos o câncer de estômago vem perdendo a importância em termos de morbi-mortalidade em países considerados de primeiro mundo. Isso não ocorre no Brasil. Os principais fatores de risco evidenciados a partir de estudos epidemiológicos em várias populações do mundo e associados a essa neoplasia são alguns padrões de dieta. Com a descoberta do papel carcinogênico das nitrosaminas e do potencial anti-oxidante da vitamina C, algumas das associações entre câncer gástrico e padrões de dieta passaram a ser parcialmente entendidas. Com a descrição da Helicobacter pylori em 1983 e as evidências da relação dessa bactéria com certos processos patológicos do estômago, alguns precursores do câncer gástrico, novos elementos foram agregados ao processo fisiopatológico dessa entidade. O conhecimento hoje adquirido sobre a fisiopatologia do câncer gástrico, mesmo que parcial, fornece perspectivas estimulantes para prevenção e diagnóstico precoce.

  4. Câncer de estômago: fatores de risco

    Directory of Open Access Journals (Sweden)

    Britto Anna Valéria de

    1997-01-01

    Full Text Available Há cinqüenta anos o câncer de estômago vem perdendo a importância em termos de morbi-mortalidade em países considerados de primeiro mundo. Isso não ocorre no Brasil. Os principais fatores de risco evidenciados a partir de estudos epidemiológicos em várias populações do mundo e associados a essa neoplasia são alguns padrões de dieta. Com a descoberta do papel carcinogênico das nitrosaminas e do potencial anti-oxidante da vitamina C, algumas das associações entre câncer gástrico e padrões de dieta passaram a ser parcialmente entendidas. Com a descrição da Helicobacter pylori em 1983 e as evidências da relação dessa bactéria com certos processos patológicos do estômago, alguns precursores do câncer gástrico, novos elementos foram agregados ao processo fisiopatológico dessa entidade. O conhecimento hoje adquirido sobre a fisiopatologia do câncer gástrico, mesmo que parcial, fornece perspectivas estimulantes para prevenção e diagnóstico precoce.

  5. Detection of RNA structures in porcine EST data and related mammals

    DEFF Research Database (Denmark)

    Seemann, Ernst Stefan; Gilchrist, Michael J.; Hofacker, Ivo L.

    2007-01-01

    -Danish PigEST resource http://pigest.ku.dk which also contains expression information distributed on 97 non-normalized cDNA libraries. RESULTS: We constructed a pipeline, EST2ncRNA, to search for known and novel ncRNAs. The pipeline utilises sequence similarity to ncRNA databases (blast), structure...... and expression in at least ten of the non-normalised cDNA libraries. We conclude that the contigs with RNAz and known predictions are in general expressed at a much lower level than protein coding transcripts. In addition, we also observe that our ncRNA candidates constitute about one to two percent of the genes...... expressed in the cDNA libraries. Intriguingly, the cDNA libraries from developmental (brain) tissues contain the highest amount of ncRNA candidates, about two percent. These observations are related to existing knowledge and hypotheses about the role of ncRNAs in higher organisms. Furthermore, about 80...

  6. EST and transcriptome analysis of cephalochordate amphioxus--past, present and future.

    Science.gov (United States)

    Wang, Yu-Bin; Chen, Shu-Hwa; Lin, Chun-Yen; Yu, Jr-Kai

    2012-03-01

    The cephalochordates, commonly known as amphioxus or lancelets, are now considered the most basal chordate group, and the studies of these organisms therefore offer important insights into various levels of evolutionary biology. In the past two decades, the investigation of amphioxus developmental biology has provided key knowledge for understanding the basic patterning mechanisms of chordates. Comparative genome studies of vertebrates and amphioxus have uncovered clear evidence supporting the hypothesis of two-round whole-genome duplication thought to have occurred early in vertebrate evolution and have shed light on the evolution of morphological novelties in the complex vertebrate body plan. Complementary to the amphioxus genome-sequencing project, a large collection of expressed sequence tags (ESTs) has been generated for amphioxus in recent years; this valuable collection represents a rich resource for gene discovery, expression profiling and molecular developmental studies in the amphioxus model. Here, we review previous EST analyses and available cDNA resources in amphioxus and discuss their value for use in evolutionary and developmental studies. We also discuss the potential advantages of applying high-throughput, next-generation sequencing (NGS) technologies to the field of amphioxus research.

  7. Application of remifentanil for conscious sedation and analgesia in short-term ERCP and EST surgery.

    Science.gov (United States)

    Sun, Guo-Qiang; Gao, Bao-Feng; Li, Guan-Jun; Lei, Yun-Long; Li, Jie

    2017-04-01

    This study aims to observe and evaluate the use of remifentanil in conscious sedation and analgesia for the safety and comfort of patients undergoing short-term endoscopic retrograde cholangiopancreatography (ERCP) and endoscopic sphincterotomy (EST).Sixty-eight patients who underwent ERCP and EST were randomly divided into two groups: research group and control group. Patients in the research group were intravenously injected with remifentanil (80-2/3* age) for 1 to 2 minutes, combined with the intravenous injection of propofol (20-30 mg) during the course of treatment. ERCP surgery was performed when Ramsay sedation scale (RSS) score reached 2-3. During the surgery, patients were closely monitored for cough symptoms, aspiration, and respiratory and circulatory system performance, and timely treatment was performed. Sedative drugs were not given in patients in the control group.In research group, the circulatory and respiratory depression of patients was mild, only one patient needed to be treated, and there was no arrhythmia requiring treatment. Five patients had respiratory depression (blood oxygen saturation decreased to bear promise for a widespread use in future clinical practice.

  8. Estéticas del exilio: el debate acerca del expresionismo

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    María Verónica Galfione

    2015-06-01

    Full Text Available El presente trabajo reconstruye los aspectos fundamentales del debate acerca del problema del expresionismo que tuvo lugar a mediados de la década del 30 entre diversos intelectuales alemanes de izquierda que se hallaban exiliados. Este debate, que fue protagonizado por autores como Georg Lukács, Ernst Bloch, Anna Seghers o Bertolt Brecht, se halló orientado a determinar el modo en que debía ser concebido un arte de carácter auténticamente revolucionario. En este contexto, resulta de nuestro interés referirnos a los aportes de Ernst Bloch y de Georg Lukács en la medida en que estos ilustran los dos extremos entre los cuales se debatió el pensamiento marxista de la década del 30.  Por un lado, la defensa del arte de vanguardia y la apuesta a descubrir un nuevo tipo de registro temporal. Por el otro, la apelación al realismo del siglo XIX y el intento de rescatar la sincronía histórica y la expectativa de un desarrollo inmanente del socialismo a partir de las contradicciones de las sociedades capitalistas avanzadas.

  9. Origens do realismo na teoria estética marxista do entreguerras

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    Marcos Rogério Cordeiro

    2016-12-01

    Full Text Available Resumo: O objetivo deste artigo é apontar como percursos intelectuais distintos, na primeira metade do século XX, levaram importantes nomes da teoria estética a um método marxista comum, incorporado às discussões literárias no período entreguerras. Nesse sentido, busca-se demonstrar como o período formativo mais idealista do círculo de Bakhtin e dos formalistas russos, bem como o de György Lukács e de Walter Benjamin, não impediram que suas obras culminassem numa noção mais dialética e historicizada sobre o conceito estético de realismo. Assim, buscaremos enfatizar que a ideia de uma condensação não mecânica da realidade na forma artística é a baliza central de todos esses autores, e é o que, em última instância, mais unifica que aparta suas distintas teorias. Palavras-chave: Realismo, Marxismo, Bakhtin, Lukács, Benjamin. 

  10. Enhanced rhamnolipid production by Pseudomonas aeruginosa overexpressing estA in a simple medium.

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    Leticia Dobler

    Full Text Available A modified Pseudomonas aeruginosa strain capable of overexpressing the estA gene, an encoding gene for a membrane-bound esterase, was constructed and its rhamnolipid (RML production was studied. Fermentations using wild-type (WT and modified P. aeruginosa strains were conducted until exhaustion of glycerol in Medium Salt Production, using two different C/N ratios. At a C/N of 83.2, the modified strain produced up to 3.9 times more RMLs than the WT, yielding a maximum concentration of 14.62 g/L RML when measured by HPLC and 22 g/L by the orcinol assay. Cell-free supernatant from the modified strain reduced surface tension to 29.4 mN/m and had a CMC of 240 mg/L and CMD of 56.05. This is the first report on the construction of an estA-based recombinant strain for RML production.

  11. Estímulo ao desenvolvimento infantil: produção do conhecimento em enfermagem

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    Bruna Cristine Peres Falbo

    2012-02-01

    Full Text Available Este estudo teve como objetivo identificar as ações de enfermagem para estímulo ao desenvolvimento infantil, em periódicos nacionais e internacionais, no período de 2000 a 2009. Revisão integrativa da literatura, nas bases de dados Medline e Lilacs, com análise de quinze artigos. Os resultados apontam o lúdico como essencial ao desenvolvimento infantil, devendo ser explorado pela enfermagem por meio da arte, música, brinquedos e teatro. Amenizar o estresse da hospitalização com adequação ambiental reduz o seu impacto sobre o desenvolvimento infantil. Orientar e intervir nos inter-relacionamentos são ações de enfermagem para o desenvolvimento social e comportamental infantil. Os temas abordados na literatura são fundamentais para uma prática de enfermagem no cuidado integral à saúde da criança.

  12. L’effet, c'est moi: Marguerite Yourcenar lectrice de Nietzsche

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    May Chehab

    2008-07-01

    Full Text Available La déconstruction nietzschéenne de la philosophie du sujet traverse toute l’œuvre de Marguerite Yourcenar : par un doute constant quant à la validité philosophique de la tradition occidentale du ‘moi’ ; par la conviction que le ‘je’ est un fait de langage et l’une des grandes erreurs de l’entendement intellectualiste face à l’intuitionnisme des philosophies orientales ; par la substitution d’une constellation du sujet à la place du royaume de l’unique. Naître à soi n’est plus soumission religieuse mais exigence personnelle d’élévation morale, dont l’accomplissement nécessite que l’on acquiesce à son destin, ceci constituant la seule liberté accessible au genre humain. À la transcendance chrétienne vient se substituer une immanence esthétique qui donne forme au projet yourcenarien de connaissance de soi.

  13. Estágio curricular supervisionado: análise da experiência discente

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    Tiago Cristiano de Lima

    2014-01-01

    Full Text Available Estudo qualitativo cujo objetivo foi analisar a experiência de estudantes no desenvolvimento das atividades do Estágio Curricular Supervisionado do curso de graduação em enfermagem de uma universidade pública do estado de São Paulo. O material foi constituído pelo relatório final dos discentes sobre o estágio desenvolvido. A análise documental foi utilizada para compreender as experiências vivenciadas pelos estudantes. Foram analisados seis documentos, utilizando-se como referencial a análise de conteúdo temático, na tentativa de identificar significados e obter inferências sobre os documentos produzidos. Os relatos permitiram concluir que a experiência dos discentes foi positiva, contribuindo para a construção de um elo entre a teoria oferecida pela universidade e a prática do serviço. O crescimento pessoal e profissional obtido foi referido pelos estudantes como fundamental para se sentirem capacitados a atuar como profissionais seguros e competentes.

  14. Molecular and functional characterization of mulberry EST encoding remorin (MiREM) involved in abiotic stress.

    Science.gov (United States)

    Checker, Vibha G; Khurana, Paramjit

    2013-11-01

    Group1 remorins may help the plants to optimize their growth under adverse conditions by their involvement in mediating osmotic stress responses in plants. Mulberry (Morus indica), a deciduous woody tree, serves as the cardinal component of the sericulture industry. Genomic endeavors in sequencing of mulberry ESTs provided clues to stress-specific clones, but their functional relevance remains fragmentary. Therefore in this study, we assessed the functional significance of a remorin gene family member that was identified in leaf ESTs. Remorins represent a large, plant-specific multigene family gaining importance in recent times with respect to their role in plant-microbe interactions, although their role in response to environmental stresses remains speculative as in vivo functions of remorin genes are limited. Mulberry remorin (MiREM) localizes to plasma membrane and is ubiquitously present in all plant organs. Expression analysis of MiREM by northern analysis reveals that its transcript increases under different abiotic stress conditions especially during dehydration and salt stress, implicating it in regulation of stress signaling pathways. Concomitantly, transgenic Arabidopsis plants overexpressing heterologous remorin show tolerance to dehydration and salinity at the germination and seedling stages as revealed by percentage germination, root inhibition assays, fresh weight and activity of photosystem II. This study predicts the possible function of group 1 remorin gene in mediating osmotic stress thus bringing novel perspectives in understanding the function of remorins in plant abiotic stress responses.

  15. Comprehensive comparisons of the current human, mouse, and rat RefSeq, Ensembl, EST, and FANTOM3 datasets

    DEFF Research Database (Denmark)

    Nordström, Karl J V; Mirza, Majd A I; Larsson, Thomas P

    2006-01-01

    . The results indicate large discrepancies between the datasets, as considerable numbers of unique transcripts can be found in each dataset. Despite the high number of ncRNA (38,129 in mouse) there are also almost 20,000 EST clusters in both mouse and humans with more than one EST that do not overlap any...

  16. Characterization and Development of EST-SSRs by Deep Transcriptome Sequencing in Chinese Cabbage (Brassica rapa L. ssp. pekinensis

    Directory of Open Access Journals (Sweden)

    Qian Ding

    2015-01-01

    Full Text Available Simple sequence repeats (SSRs are among the most important markers for population analysis and have been widely used in plant genetic mapping and molecular breeding. Expressed sequence tag-SSR (EST-SSR markers, located in the coding regions, are potentially more efficient for QTL mapping, gene targeting, and marker-assisted breeding. In this study, we investigated 51,694 nonredundant unigenes, assembled from clean reads from deep transcriptome sequencing with a Solexa/Illumina platform, for identification and development of EST-SSRs in Chinese cabbage. In total, 10,420 EST-SSRs with over 12 bp were identified and characterized, among which 2744 EST-SSRs are new and 2317 are known ones showing polymorphism with previously reported SSRs. A total of 7877 PCR primer pairs for 1561 EST-SSR loci were designed, and primer pairs for twenty-four EST-SSRs were selected for primer evaluation. In nineteen EST-SSR loci (79.2%, amplicons were successfully generated with high quality. Seventeen (89.5% showed polymorphism in twenty-four cultivars of Chinese cabbage. The polymorphic alleles of each polymorphic locus were sequenced, and the results showed that most polymorphisms were due to variations of SSR repeat motifs. The EST-SSRs identified and characterized in this study have important implications for developing new tools for genetics and molecular breeding in Chinese cabbage.

  17. Statistics information of rice EST mapping results - RGP estmap2001 | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us RGP estmap2001 Statistics information of rice EST mapping results Data detail Data name Statistics...of This Database Site Policy | Contact Us Statistics information of rice EST mapping results - RGP estmap2001 | LSDB Archive ...

  18. Research

    African Journals Online (AJOL)

    abp

    hypertension artérielle (HTA) est la pathologie et le facteur de risque cardiovasculaire le plus important. Actuellement, on estime qu'environ un milliard de personnes dans le monde souffre d'. HTA et ce chiffre devrait encore augmenter ...

  19. Malformations vasculaires au cours du syndrome de Williams ...

    African Journals Online (AJOL)

    Le syndrome de Williams-Beuren est une maladie génétique rare, il associe classiquement une dysmorphie faciale assez spécifique, des malformations cardiovasculaires et un profil neuropsychologique particulier. Nous rapportons les observations de trois enfants atteints du syndrome de Williams-Beuren en insistant ...

  20. Isolation and characterization of a novel cold-adapted esterase, MtEst45, from Microbulbifer thermotolerans DAU221

    Directory of Open Access Journals (Sweden)

    Yong-Suk eLee

    2016-03-01

    Full Text Available A novel esterase, MtEst45, was isolated from a fosmid genomic library of Microbulbifer thermotolerans DAU221. The encoding gene is predicted to have a mass of 45,564 Da and encodes 495 amino acids, excluding a 21 amino acid signal peptide. MtEst45 showed a low amino acid identity (approximately 23–24% compared with other lipolytic enzymes belonging to Family III, a closely related bacterial lipolytic enzyme family. MtEst45 also showed a conserved GXSXG motif, G131IS133YG135, which was reported as active site of known lipolytic enzymes, and the putative catalytic triad composed of D237 and H265. Because these mutants of MtEst45, which was S133A, D237N, and H265L, had no activity, these catalytic triad essential for the enzyme catalysis. MtEst45 was overexpressed in Escherichia coli BL21 (DE3 and purified via His-tag affinity chromatography. The optimal pH and temperature of MtEst45 were estimated to be 8.17 and 46.27°C by response surface methodology, respectively. Additionally, MtEst45 was also active between 1–15°C. The optimal hydrolysis substrate for MtEst45 among p-nitrophenyl esters (C2–C18 was p-nitrophenyl butyrate, and the Km and Vmax values were 0.0998 mM and 550 μmol/min/mg of protein, respectively. MtEst45 was strongly inhibited by Hg2+, Zn2+, and Cu2+ ions; by phenylmethanesulfonyl fluoride; and by β-mercaptoethanol. Ca2+ did not affect the enzyme’s activity. These biochemical properties, sequence identity, and phylogenetic analysis suggest that MtEst45 represents a novel and valuable bacterial lipolytic enzyme family and is useful for biotechnological applications.

  1. Es posible el arte sin estética una aproximación al problema de la estética en el libro ¿Qué es el arte? de Arthur Danto

    Directory of Open Access Journals (Sweden)

    Edison Francisco Viveros Chavarría

    2016-01-01

    Full Text Available Este artículo de reflexión se ocupa de analizar la noción de estética en el arte expuesta por Danto en el texto “¿Qué es el arte?”. La hipótesis de lectura es que el arte es posible sin estética si por estética se entiende sólo la exposición de lo bello que da placer visual. Sin embargo, el arte no se puede separar de la estética, si ésta es comprendida como la manera en que las partes de una obra se muestran al mismo tiempo que la justificación para exponerlas de una forma o de otra. Se concluye que la clave para la solución del problema referido a si es posible el arte sin estética, se encuentra en la precisión filosófica, la consistencia y el sentido de pluralidad que Danto le da a la noción de significado encarnado en contraste, pero no en la incompatibilidad con la estética como exposición de lo bello que da placer visual. La estética es indisoluble del arte sí y sólo si lo bello no es el eje central de esta relación, sino algo que hace parte de la expresión artística. Estética no es sinónimo de belleza. Es decir, la belleza puede ser eludible para el arte. Sin embargo, la belleza no puede ser separada de la vida humana porque constituye un valor para los hombres porque es el encuentro positivo entre éstos.

  2. Estéticas tecnológicas en la imagen educativa

    Directory of Open Access Journals (Sweden)

    Kuchen, Regina Elena

    2012-01-01

    Full Text Available [es] Las estéticas tecnológicas son tomadas como puerta de entrada para el estudio de las imágenes destinadas a la transmisión de conocimiento. El análisis de un libro sobre el cuerpo humano es el pretexto para desarrollar la hipótesis de que las estéticas tecnológicas exteriorizan fenómenos más profundos y generales, que lejos de acotarse a la inscripción de éstas en el imaginario técnico contemporáneo, dan cuenta de la dinámica interna de la cultura visual.El propósito de estas observaciones, más allá de la descripción de las estéticas tecnológicas en el entramado de relaciones entre los códigos de la percepción, de la representación y del saber (planos de codificación relevantes en nuestra cultura visual, es contribuir a reconsiderar el potencial cognitivo de las imágenes para pensar proyectivamente la producción de textos visuales que exploten la potencialidad lúdica, heurística y sinérgica de la imagen, en la transmisión de conocimiento. [en] Technological esthetics is considered a door that enables the study of images aimed at knowledge transmission. The analysis of a book about a human body is an excuse for the hypothesis development that techological esthetics externalizes deeper and broader phenomena that far from being restricted to its inscription in contemporary technical representations account for the internal dynamics of the visual culture.The aim of these observations beyond the description of technological esthetics in a web wound round relations among perception, representation and knowledege codes (codification planes relevant to our visual culture is to contribute to a reappraisal of the cognitive potential of images in order to think in a prospective way about the production of visual texts that exploit the playful, heuristic and synergetic potentiality of images for knowledge transmission.

  3. An elm EST database for identifying leaf beetle egg-induced defense genes

    Directory of Open Access Journals (Sweden)

    Büchel Kerstin

    2012-06-01

    Full Text Available Abstract Background Plants can defend themselves against herbivorous insects prior to the onset of larval feeding by responding to the eggs laid on their leaves. In the European field elm (Ulmus minor, egg laying by the elm leaf beetle ( Xanthogaleruca luteola activates the emission of volatiles that attract specialised egg parasitoids, which in turn kill the eggs. Little is known about the transcriptional changes that insect eggs trigger in plants and how such indirect defense mechanisms are orchestrated in the context of other biological processes. Results Here we present the first large scale study of egg-induced changes in the transcriptional profile of a tree. Five cDNA libraries were generated from leaves of (i untreated control elms, and elms treated with (ii egg laying and feeding by elm leaf beetles, (iii feeding, (iv artificial transfer of egg clutches, and (v methyl jasmonate. A total of 361,196 ESTs expressed sequence tags (ESTs were identified which clustered into 52,823 unique transcripts (Unitrans and were stored in a database with a public web interface. Among the analyzed Unitrans, 73% could be annotated by homology to known genes in the UniProt (Plant database, particularly to those from Vitis, Ricinus, Populus and Arabidopsis. Comparative in silico analysis among the different treatments revealed differences in Gene Ontology term abundances. Defense- and stress-related gene transcripts were present in high abundance in leaves after herbivore egg laying, but transcripts involved in photosynthesis showed decreased abundance. Many pathogen-related genes and genes involved in phytohormone signaling were expressed, indicative of jasmonic acid biosynthesis and activation of jasmonic acid responsive genes. Cross-comparisons between different libraries based on expression profiles allowed the identification of genes with a potential relevance in egg-induced defenses, as well as other biological processes, including signal transduction

  4. ¿Lo que este texto quiere [o no quiere] decir?

    Directory of Open Access Journals (Sweden)

    Daiane Lopes

    2014-07-01

    Full Text Available Sabemos que la literatura suscita conocimiento. El proceso de aprendizaje a través de la lectura literaria acompaña todas las fases de la vida. Sin embargo, una definición para ese tipo de conocimiento es compleja, ya que él es subjetivo y se manifiesta de modo muy individual. Eso ocurre debido al diálogo que pasa entre texto, autor y lector. Se cada individuo carga consigo un texto, al leer, nuestra producción de sentidos irá diferir de la producción de los demás lectores. De esa forma, expondremos, en el presente estudio, algunas reflexiones sobre el conocimiento propiciado por la lectura literaria. Primeramente, presentaremos una síntesis del cuento “En Casa”, de Anton Pavlovitch Tchekhov, texto que motivó la realización del artículo y que servirá de ejemplo para todas las ponderaciones presentadas. Enseguida, realizaremos algunas consideraciones sobre la literatura infantil, considerándola como el inicio del contacto con el texto literario. Argumentaremos sobre los efectos de la literatura tanto en adultos como en niños, así como sobre las maneras de presentación de tal arte al lector. Finalmente, intentaremos caracterizar el conocimiento provocado por la literatura, teniendo como base, entre otros, autores como Vigotsky y Umberto Eco. El texto es compuesto de cuestionamientos y de posibles respuestas, ya que no posee la intención de exhibir convicciones, pero de promover reflexiones sobre la temática. En nuestra investigación teórica, percibimos, entonces, que no es posible contestar a la indagación sobre “lo que un texto quiere decir”, cuestionamiento que intitula el artigo. Tal respuesta irá sufrir variaciones todas las veces que la lectura fuere hecha por lectores diferentes o hasta mismo cuando el mismo lector leer el mismo texto otra vez. Las posibles respuestas, en realidad, estarán respondiendo a una otra cuestión: ¿cómo es el conocimiento propiciado por la literatura? Para este, presentaremos

  5. The EVER-EST Virtual Research Environment for the European Volcano Supersites

    Science.gov (United States)

    Salvi, S.; Trasatti, E.; Rubbia, G.; Romaniello, V.; Marelli, F.

    2017-12-01

    EVER-EST (European Virtual Environment for Research - Earth Science Themes) is an European H2020 project (2015-2018) aimed at the creation of a Virtual Research Environment (VRE) for the Earth Sciences. The VRE is intended to enhance the ability to collaborate and share knowledge and experience among scientists. One of the innovations of the project is the exploitation of the "Research Object" concept (http://www.rohub.org). Research Objects encapsulate not only data and publications, but also algorithms, codes, results, and workflows that can be stored, shared and re-used. Four scientific communities are involved in the EVER-EST project: land monitoring, natural hazards, marine biology, and the GEO Geohazard Supersites community (http://www.earthobservations.org/gsnl.php). The latter is represented in the project by INGV and the University of Iceland, and has provided user requirements to tailor the VRE to the common needs of the worldwide Supersite communities. To develop and test the VRE we have defined user scenarios and created Research Objects embedding research activities and workflows on the Permanent Supersites Campi Flegrei, Mount Etna and Icelandic Volcanoes (http://vm1.everest.psnc.pl/supersites/). While these Supersites are test sites for the platform, during the last year of the project other Supersites may also be involved to demonstrate the added value of the collaborative environment in research activities aiming to support Disaster Risk Reduction. Using the VRE, scientists are able to collaborate with colleagues located in different parts of the world, in a simple and effective way. This includes being able to remotely access and share data, research results and ideas, to carry out training sessions and discussions, to compare different results and models, and to synthesize many different pieces of information in a single consensus product to be disseminated to end-users. In particular, a further need of the Supersite scientists, which can be

  6. An elm EST database for identifying leaf beetle egg-induced defense genes.

    Science.gov (United States)

    Büchel, Kerstin; McDowell, Eric; Nelson, Will; Descour, Anne; Gershenzon, Jonathan; Hilker, Monika; Soderlund, Carol; Gang, David R; Fenning, Trevor; Meiners, Torsten

    2012-06-15

    Plants can defend themselves against herbivorous insects prior to the onset of larval feeding by responding to the eggs laid on their leaves. In the European field elm (Ulmus minor), egg laying by the elm leaf beetle ( Xanthogaleruca luteola) activates the emission of volatiles that attract specialised egg parasitoids, which in turn kill the eggs. Little is known about the transcriptional changes that insect eggs trigger in plants and how such indirect defense mechanisms are orchestrated in the context of other biological processes. Here we present the first large scale study of egg-induced changes in the transcriptional profile of a tree. Five cDNA libraries were generated from leaves of (i) untreated control elms, and elms treated with (ii) egg laying and feeding by elm leaf beetles, (iii) feeding, (iv) artificial transfer of egg clutches, and (v) methyl jasmonate. A total of 361,196 ESTs expressed sequence tags (ESTs) were identified which clustered into 52,823 unique transcripts (Unitrans) and were stored in a database with a public web interface. Among the analyzed Unitrans, 73% could be annotated by homology to known genes in the UniProt (Plant) database, particularly to those from Vitis, Ricinus, Populus and Arabidopsis. Comparative in silico analysis among the different treatments revealed differences in Gene Ontology term abundances. Defense- and stress-related gene transcripts were present in high abundance in leaves after herbivore egg laying, but transcripts involved in photosynthesis showed decreased abundance. Many pathogen-related genes and genes involved in phytohormone signaling were expressed, indicative of jasmonic acid biosynthesis and activation of jasmonic acid responsive genes. Cross-comparisons between different libraries based on expression profiles allowed the identification of genes with a potential relevance in egg-induced defenses, as well as other biological processes, including signal transduction, transport and primary metabolism

  7. Ética y estética (Novenas Conferencias Aranguren, 2000

    Directory of Open Access Journals (Sweden)

    Trías, Eugenio

    2001-12-01

    Full Text Available Wittgenstein's Tractatus contains a mysterious aphorism to the effect that «ethics and aesthetics are the same». The existence of an underlying connivance between ethics and aesthetics has been acknowledged ever since ancient times. Here we try to look into this hard and interesting issue from the open horizon of boundary-philosophy, as put forward in my latest publications. The task will be carried out at three stages. The first one is given over to an elucidation of one core concept of ethics, freedom, in an interactive connection with our fore-understanding of our human condition. Then we raise the (Kantian question about how the ethical «symbolically resounds» through art. The third and last stage brings up the subject of the link between ethics and aesthetics on the ground of several examples, both ancient and modern (from the great tragedies and some artistic manifestations of our modernity.

    Un misterioso aforismo del Tractatus de Wittgenstein enuncia que «ética y estética son lo mismo». Que una corriente de complicidad existe entre ética y estética es reconocido desde la antigüedad. Lo ético se argumenta en la praxis; el arte lo hace a través de la poiésis, o creación, ya definida por Platón y Aristóteles. Aquí se intenta abordar esta interesante y difícil cuestión desde el horizonte abierto, a través de mis últimas publicaciones, por la filosofía del límite, y se realizará en tres etapas. Una primera dedicada a esclarecer uno de los conceptos nucleares de la ética, la libertad, en relación de interacción con la precomprensión que podemos poseer relativa a nuestra humana conditio. Se planteará luego la cuestión (kantiana de que a través del arte «resuena simbólicamente» lo ético. En una tercera y última etapa se desarrolla el tema del nexo entre ética y estética, dándose algunos ejemplos pertinentes, antiguos y modernos (de las grandes

  8. Métropolisation et recomposition territoriale du Nord-Est tunisien

    OpenAIRE

    Dlala, Habib

    2007-01-01

    Le report de la croissance du Grand Tunis sur sa zone d’influence immédiate s’est opéré tardivement de manière extensive et en l’absence d’une fonctionnalité fondée sur la division spatiale du travail et d’un différentiel de qualité suffisamment tranché. Les processus à l’oeuvre dans les zones périurbaines et rurales avoisinantes ainsi que la satellisation des villes petites et moyennes situées à 30-40 et 60-70 kilomètres autour de la capitale créent une vaste zone de solidarité métropolitain...

  9. Entidades socioafetivas: do casamento à união estável e à monoparentalidade

    OpenAIRE

    Russo, José

    2005-01-01

    Esta dissertação tem como objetivo fazer uma releitura das entidades familiares, passando pelo casamento, pela união estável e pela família monoparental. De início, é feita uma abordagem socioantropológica da família, para, em seguida, aprofundar uma análise do instituto do casamento em suas formas de constituição, seus efeitos jurídicos e seus aspectos de dissolução. Serão também examinadas as dificuldades enfrentadas pela sociedade brasileira na aceitação da proteção legal à ...

  10. Capitalismo, esquizofrenia y consenso de la estética relacional

    Directory of Open Access Journals (Sweden)

    Eric Alliez

    2006-10-01

    Full Text Available Este ensayo presenta un análisis crítico del llamado “arte relacional” y de la ruptura de éste con las vanguardias modernas. Para el desarrollo de la crítica, el autor da cuenta de la situación paradójica de las prácticas artísticas contemporáneas y su fundamento discursivo y “amigable”. Finalmente, señala los compromisos del arte contemporáneo con una lógica del consenso y del intercambio mercantilista, en tanto la reducción de la sensación a la opinión, el acontecimiento a la exposición y el concepto a la mercancía, en la obra de arte, en la “posmodernidad”.

  11. Controle los mosquitos que están en el exterior (Controlling Mosquitoes Outside)

    Centers for Disease Control (CDC) Podcasts

    2016-07-11

    Los mosquitos pueden transmitir virus como el del zika. En este podcast, el Sr. Francisco le enseñará a usted y a su vecina Adriana diferentes maneras para ayudar a reducir la cantidad de mosquitos fuera de su casa. Los consejos incluyen eliminar áreas de agua estancada donde los mosquitos ponen sus huevos, usar larvicidas para matar mosquitos jóvenes, y reparar grietas y cubrir las ventilaciones de los pozos sépticos. También aprenderá cómo se usan los aviones que ayudan a rociar insecticida para los mosquitos.  Created: 7/11/2016 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 7/11/2016.

  12. Alongamento estático ativo no desempenho em provas de potência e velocidade

    OpenAIRE

    Oliveira, Fábio Carlos Lucas de

    2011-01-01

    Dissertação de mestrado em Biocinética, apresentada à Faculdade de Ciências do Desporto e Educação Física da Universidade de Coimbra O propósito deste estudo foi avaliar a influência das tarefas de alongamento estático ativo em provas de potência e velocidade como o “salto vertical com contra-movimento” (CMJ) e o “sprint de 20 metros”. A amostra foi composta por 22 atletas, do sexo masculino, praticantes de rugby7 e handebol, com média de idade 24 ± 6,2 anos. Os atletas foram, aleatoriamen...

  13. est "ailleurs" ? Sémantique lexicale de l'adverbe spatial "ailleurs".

    OpenAIRE

    Lammert, Marie

    2013-01-01

    Comme d’autres mots, nous pensons que l’adverbe spatial ailleurs contient deux types d’information : un sens conceptuel ou descriptif et un sens procédural ou instructionnel qui dépend du processus sémantico-référentiel à l’œuvre. Cet article postule qu’ailleurs est un adverbe anaphorique (dans une conception mémorielle de l’anaphore) et non pas un adverbe déictique (comme le pense Stein-Zintz, 2008). La résolution anaphorique nécessite alors un point de repère spatial à partir duquel la réfé...

  14. ¿De qué lado estás?

    Directory of Open Access Journals (Sweden)

    Thomas Sheehan

    2017-08-01

    Full Text Available En la época moderna se prioriza uno de nuestros dos «lados», el «lado» analítico, a expensas de nuestro «lado» más sintético e intuitivo. Las nefastas consecuencias del énfasis en la razón instrumental que empezó en la época de Francis Bacon han sido descritoselocuentemente por varios pensadores importantes, incluyendo Max Weber, Martin Heidegger y Jürgen Habermas. El descuido de nuestras capacidades imaginativas puede empobrecer nuestras producciones artísticas y científicas y puede dificultar el acceso a la experiencia religiosa. La solución pasa por fomentar las habilidades que están siendo marginadas progresivamente en el sistema educativo actual en un intento de conseguir un sano equilibrio o armonía entre las distintas habilidades cognitivas del ser humano.

  15. EXPERIÊNCIA E DOCUMENTAÇÃO: É POSSÍVEL ARTICULAR ESTES CONCEITOS?

    OpenAIRE

    Cláudia Inês Horn; Jacqueline Silva da Silva

    2011-01-01

    Este trabalho apresenta o conceito de experiência fundamentado em Larrosa (2002, 2004) e a sua articulação com a documentação pedagógica. A experiência é o que nos passa, ou o que nos acontece, ou o que nos toca. O sujeito da experiência seria como um território de passagem, na qual aquilo que passa afeta de algum modo. A documentação pedagógica é vista como uma possibilidade de tornar visível a construção da memória do grupo de crianças em seu contexto escolar, trazendo os conceitos de obser...

  16. Porrinha: quando as probabilidades estão além de dados e moedas

    Directory of Open Access Journals (Sweden)

    Paulo Guilherme Pinheiro dos Santos

    2014-06-01

    Full Text Available A utilização de jogos que fazem parte do cotidiano dos alunos é muito importante para o interesse e aprendizado de qualquer disciplina. O jogo denominado porrinha foi utilizado com o objetivo de modelar um caso de cálculo das probabilidades. Este trabalho é classificado como ensaio teórico e é mostrado o desenvolvimento da esperança matemática da variável considerada neste jogo, a soma. Alguns resultados para dois, três e quatros jogadores são desenvolvidos. O jogo da porrinha é adequado para criar uma sequência didática para o ensino de probabilidade

  17. A estética documental no cinema ficcional de horror

    OpenAIRE

    Amanda Neves de Oliveira

    2015-01-01

    O presente trabalho tem como proposta estudar o uso de estruturas documentais no cinema ficcional de horror, tipo de narrativa que ficou conhecida popularmente como found footage. Esses filmes fazem uso de uma linguagem propositalmente híbrida, associando a forma do documentário ao conteúdo da ficção e, hoje, encontram-se tão em voga que já possuem até mesmo clichês e estereótipos. A intenção é entender de que forma o gênero do horror se apropria douso de uma estética associada a registros do...

  18. El problema de la consultoría está en la t

    Directory of Open Access Journals (Sweden)

    Julio César Alonso

    2013-01-01

    Full Text Available El objetivo de este caso es reforzar la capacidad del lector de aproximarse de forma sistémica a un proble- ma que requiere el uso de herramientas econométricas, en especial la regresión múltiple. Para ello, el pro- blema se contextualiza en una empresa consultora que debe estimar una función de demanda y debe de- terminar qué pruebas de hipótesis deben hacerse para los coeficientes del modelo de regresión lineal estimado. Esta discusión toca un tema que parece darse por sentado, pero que refleja la necesidad de re- flexionar acerca de las razones por las cuales se hace uso de una prueba t individual de 2 colas o de 1 cola y la relación con la teoría económica.

  19. Relatório de estágio em surf na equipa Surftechnique

    OpenAIRE

    Moisés, João Alexandre Fernandes

    2014-01-01

    Mestrado em Treino Desportivo O relatório de estágio profissional foi uma análise do treino, da performance e da competição no surf. Dividiu-se em 4 áreas distintas, que são, a observação e analise do treino, a periodização e preparação física, a investigação e a competição. Estas ligam-se entre si e retractam as actividades desenvolvidas nas épocas 2011 e 2012, na Surftechnique, quer no grupo de competição, quer no atleta em particular. O primeiro passo foi a observação e analise do...

  20. O estético em Fazenda Modelo: novela pecuária, de Chico Buarque

    OpenAIRE

    Silva, Vitoria Regina Xavier da

    2009-01-01

    O estudo realizado nesta dissertação buscou a valorização do projeto estético de Chico Buarque em Fazenda Modelo: novela pecuária (1975), narrativa de estréia do autor, considerando que a leitura feita pela crítica literária destaca motivações políticas referentes ao contexto histórico de produção do Brasil da década de 1970, marcado pelo regime militar. Nossa leitura voltou-se para o plano de ficção narrativa e aos elementos de composição da montagem do texto. Com o inventário das escolhas t...

  1. Estados alterados de consciencia: lo que la fMRI está revelando

    Directory of Open Access Journals (Sweden)

    Alba Martínez López

    2015-07-01

    Full Text Available El diagnóstico diferencial de los estados alterados de consciencia (coma, estados vegetativos y estados de mínima consciencia es complicado y puede provocar numerosas confusiones. Las técnicas de neuroimagen actuales están aportando información novedosa no sólo en el diagnóstico, sino también en ámbitos conceptuales y de rehabilitación. Revisamos aquí un estudio reciente que utiliza resonancia magnética funcional (fMRI para mostrar que el nivel de consciencia real de un paciente puede ser diferente al que indica una técnica comportamental clásica. Asimismo, se abren nuevas vías a la hora de poder establecer algún tipo de comunicación de estas personas con el medio exterior.

  2. La Primera Guerra Mundial. Un conflicto que llega desde el Este

    Directory of Open Access Journals (Sweden)

    Carmen Scocozza

    2015-07-01

    Full Text Available El aniversario de la PGM representó una oportunidad para reflexionar sobre la actualidad de un conflicto que cambió radicalmente la fisionomía del mapa europeo. En particular, a través de una reconstrucción historiográfica que coloca las raíces de la guerra en el Este de Europa. Desde esta perspectiva, se quiere analizar cómo las problemáticas presentes en Europa Oriental se convirtieron en el detonante de una crisis generalizada. En ese orden, se pretende destacar que el conflicto tuvo un carácter más radical en los territorios herederos de políticas imperiales seculares y que produjo consecuencias desestabilizadoras que siguen afectando los equilibrios mundiales.

  3. Le dessalement de l’eau de mer est-il une voie d’avenir?

    OpenAIRE

    Jean-Noël Salomon

    2012-01-01

    La pénurie en eau semble devenir une perspective inquiétante pour l’humanité. Aujourd’hui les pays en situation de stress hydrique sont de plus en plus nombreux tandis que les besoins ne cessent de grandir. Face à ce constat, nombre de solutions techniques ont été apportées dont celle du dessalement de l’eau de mer. Cette activité est en pleine expansion du fait d’une amélioration constante des techniques et d’un coût de plus en plus réduit. Déjà le dessalement représente la principale source...

  4. Development of 198 novel EST-derived microsatellites in Eucalyptus (Myrtaceae).

    Science.gov (United States)

    He, Xudong; Wang, Yu; Li, Fagen; Weng, Qijie; Li, Mei; Xu, Li-an; Shi, Jisen; Gan, Siming

    2012-04-01

    Expressed sequence tag (EST)-derived microsatellites were identified in Eucalyptus through screening the GenBank database. The loci were sequence-verified and explored for polymorphism among 20 genotypes. In total, 198 novel microsatellites were developed from 8262 unigenes, with the identity of 73.6-100% to the original sequences and presence of the expected repeat motifs. One hundred and eighty-four markers proved to be polymorphic among 10 E. urophylla and 10 E. tereticornis genotypes, with the number of alleles per locus, observed heterozygosity, and polymorphic information content being 2-17 (mean: 7.11), 0-1.0 (mean: 0.4511), and 0.0940-0.9131 (mean: 0.6571), respectively. These markers will be useful for germplasm characterization, genome mapping, and gene tagging for economic traits in the two species examined and may have potential for genetic applications in Eucalyptus.

  5. Câncer de estômago: fatores de risco

    OpenAIRE

    Britto,Anna Valéria de

    1997-01-01

    Há cinqüenta anos o câncer de estômago vem perdendo a importância em termos de morbi-mortalidade em países considerados de primeiro mundo. Isso não ocorre no Brasil. Os principais fatores de risco evidenciados a partir de estudos epidemiológicos em várias populações do mundo e associados a essa neoplasia são alguns padrões de dieta. Com a descoberta do papel carcinogênico das nitrosaminas e do potencial anti-oxidante da vitamina C, algumas das associações entre câncer gástrico e padrões de di...

  6. Neural Network Prediction of Translation Initiation Sites in Eukaryotes: Perspectives for EST and Genome analysis

    DEFF Research Database (Denmark)

    Pedersen, Anders Gorm; Nielsen, Henrik

    1997-01-01

    Translation in eukaryotes does not always start at the first AUG in an mRNA, implying that context information also plays a role.This makes prediction of translation initiation sites a non-trivial task, especially when analysing EST and genome data where the entire mature mRNA sequence is not known....... In this paper, we employ artificial neural networks to predict which AUG triplet in an mRNA sequence is the start codon. The trained networks correctly classified 88% of Arabidopsis and 85% of vertebrate AUG triplets. We find that our trained neural networks use a combination of local start codon context...... and global sequence information. Furthermore, analysis of false predictions shows that AUGs in frame with the actual start codon are more frequently selected than out-of-frame AUGs, suggesting that our nteworks use reading frame detection. A number of conflicts between neural network predictions and database...

  7. SNP mining porcine ESTs with MAVIANT, a novel tool for SNP evaluation and annotation

    DEFF Research Database (Denmark)

    Panitz, Frank; Stengaard, Henrik; Hornshoj, Henrik

    2007-01-01

    MOTIVATION: Single nucleotide polymorphisms (SNPs) analysis is an important means to study genetic variation. A fast and cost-efficient approach to identify large numbers of novel candidates is the SNP mining of large scale sequencing projects. The increasing availability of sequence trace data...... in public repositories makes it feasible to evaluate SNP predictions on the DNA chromatogram level. MAVIANT, a platform-independent Multipurpose Alignment VIewing and Annotation Tool, provides DNA chromatogram and alignment views and facilitates evaluation of predictions. In addition, it supports direct...... manual annotation, which is immediately accessible and can be easily shared with external collaborators. RESULTS: Large-scale SNP mining of polymorphisms bases on porcine EST sequences yielded more than 7900 candidate SNPs in coding regions (cSNPs), which were annotated relative to the human genome. Non...

  8. Defining a large set of full-length clones from a Xenopus tropicalis EST project.

    Science.gov (United States)

    Gilchrist, Michael J; Zorn, Aaron M; Voigt, Jana; Smith, James C; Papalopulu, Nancy; Amaya, Enrique

    2004-07-15

    Amphibian embryos from the genus Xenopus are among the best species for understanding early vertebrate development and for studying basic cell biological processes. Xenopus, and in particular the diploid Xenopus tropicalis, is also ideal for functional genomics. Understanding the behavior of genes in this accessible model system will have a significant and beneficial impact on the understanding of similar genes in other vertebrate systems. Here we describe the analysis of 219,270 X. tropicalis expressed sequence tags (ESTs) from four early developmental stages. From these, we have deduced a set of unique expressed sequences comprising approximately 20,000 clusters and 16,000 singletons. Furthermore, we developed a computational method to identify clones that contain the complete coding sequence and describe the creation for the first time of a set of approximately 7000 such clones, the full-length (FL) clone set. The entire EST set is cloned in a eukaryotic expression vector and is flanked by bacteriophage promoters for in vitro transcription, allowing functional experiments to be carried out without further subcloning. We have created a publicly available database containing the FL clone set and related clustering data (http://www.gurdon.cam.ac.uk/informatics/Xenopus.html) and we make the FL clone set publicly available as a resource to accelerate the process of gene discovery and function in this model organism. The creation of the unique set of expressed sequences and the FL clone set pave the way toward a large-scale systematic analysis of gene sequence, gene expression, and gene function in this vertebrate species.

  9. Evolution and comparative genomics of subcellular specializations: EST sequencing of Torpedo electric organ.

    Science.gov (United States)

    Nazarian, Javad; Berry, Deborah L; Sanjari, Salar; Razvi, Mohammed; Brown, Kristy; Hathout, Yetrib; Vertes, Akos; Dadgar, Sherry; Hoffman, Eric P

    2011-03-01

    Uncharacterized open reading frames (ORFs) in human genomic sequence often show a high degree of evolutionary conservation, yet have little or no tissue EST or protein data suggestive of protein product function. The encoded proteins may have highly restricted expression in specialized cells, subcellular specializations, and/or narrow windows during development. One such highly specialized and minute subcellular compartment is the neuromuscular junction (NMJ), where motorneurons contact muscle fibers. The electric Torpedo ray has evolved to expand the NMJ structure to the size of a large organ (electroplax organ), and we hypothesized that Torpedo electroplax proteins would be candidates for human ESTs expressed at the human NMJ. A total of 9719 primary electroplax cDNA clones were sequenced. We identified 44 human ORFs showing high (>63%) amino acid identity to Torpedo electroplax transcripts with enrichment for mRNA splicing motifs (SH2 and pre-mRNA splicing domains), an observation potentially important for the strict nuclear domains maintained by myonuclei underlying the NMJ. We generated antibodies against two uncharacterized human genes (C19orf29 [Drosophila cactin] and C15orf24) and showed that these were indeed expressed at the murine NMJ. Cactin, a member of the Rel transcription factor family in Drosophila, localized to the postsynaptic cytosol of the NMJ and nuclear membrane. C15orf24 protein localized to the murine postsynaptic sarcolemma. We show a novel approach towards identifying proteins expressed at a subcellular specialization using evolutionary diversity of organ function and cross-species mapping. Copyright © 2010 Elsevier B.V. All rights reserved.

  10. Este tiempo clausurado que no me pertenece: Los años falsos, de Josefina Vicens

    Directory of Open Access Journals (Sweden)

    Gerardo Bustamante Bermúdez

    2011-07-01

    Full Text Available En 1982, Josefina Vicens dio a conocer su segunda novela titulada Los años falsos. En este artículo se analiza el tema del patriarcado mexicano en las estructuras familiares en donde el poder, la violencia, la infidelidad, la corrupción, el machismo y la subordinación de las mujeres respecto a la autoridad del patriarca constituyen la base de un discurso incisivo en el que Vicens observa las relaciones de género dentro de la familia tradicional mexicana. A lo largo de este texto se habla sobre la identidad asignada al protagonista de la novela, así como la ambigüedad respecto a su construcción de género que, no obstante, queda al margen de sus deseos y posibles realizaciones. Se trata de una vida anulada por una especie de testamento de género por parte del patriarca ausente / presente. In 1982, Josefina Vicens published her second novel entitled The False Years. This article discusses the issue of Mexican patriarchy in family structures where power, violence, infidelity, corruption, machismo and the subordination of women with regard to the authority of the patriarch form the basis of an incisive discourse in which Vicens observes gender relations within the traditional Mexican family. The identity of the novel’s protagonist is discussed throughout the narrative, as is his ambivalence with respect to the construction of his gender, a feeling relegated to the margins of his wishes and possible acheivements. It is the portrait of a life nullified by a sort of testament of gender handed down by the absent/present figure of the patriarch.

  11. Estética del devenir adverso en la narrativa de Franz Kafka

    Directory of Open Access Journals (Sweden)

    Adriana De La Hoz

    2015-08-01

    Full Text Available En este ensayo se disertará sobre un problema que parece ser poco cuestionado en los trabajos precedentes que se han inspirado en la incomparable narrativa de Franz Kafka. Hablamos en particular de la “estética de lo adverso”, de la imposibilidad, del obstáculo, del fracaso; aspectos que se desvelan como un vector temático con desarrollo semántico y de constante aparición en la obra del escritor checo. Frente a tan intrincada semiosis, nuestra pretensión es responder parcialmente a algunas preguntas que buscan abrir espacios sígnicos que permitan hacer una lectura más amplia y, quizá, más edificante de la obra kafkiana, lo que necesariamente concluirá en el fracaso como una nueva alternativa hermenéutica, o una alternativa hermenéutica construida desde el fracaso. This essay deals with a problem that does not seem to be questioned in preceding works based on Franz Kafta’s incomparable narrative. We are specifically speaking of “the aesthetics of the adverse,” the impossibility, the obstacle, the failure; aspects that are revealed as a topic with semantic development and with permanent appearance in the work of this Czech writer. Before such a complicated semiosis, our intention is to partially respond to some questions intended to open sign spaces that allow having a wider and maybe more edifying reading of Kaffa’s work, which will necessarily result in the failure as a new hermeneutic alternative or a hermeneutic alternative built from failure.

  12. Bioinformatic screening of human ESTs for differentially expressed genes in normal and tumor tissues

    Directory of Open Access Journals (Sweden)

    Mouchiroud Dominique

    2006-04-01

    Full Text Available Abstract Background Owing to the explosion of information generated by human genomics, analysis of publicly available databases can help identify potential candidate genes relevant to the cancerous phenotype. The aim of this study was to scan for such genes by whole-genome in silico subtraction using Expressed Sequence Tag (EST data. Methods Genes differentially expressed in normal versus tumor tissues were identified using a computer-based differential display strategy. Bcl-xL, an anti-apoptotic member of the Bcl-2 family, was selected for confirmation by western blot analysis. Results Our genome-wide expression analysis identified a set of genes whose differential expression may be attributed to the genetic alterations associated with tumor formation and malignant growth. We propose complete lists of genes that may serve as targets for projects seeking novel candidates for cancer diagnosis and therapy. Our validation result showed increased protein levels of Bcl-xL in two different liver cancer specimens compared to normal liver. Notably, our EST-based data mining procedure indicated that most of the changes in gene expression observed in cancer cells corresponded to gene inactivation patterns. Chromosomes and chromosomal regions most frequently associated with aberrant expression changes in cancer libraries were also determined. Conclusion Through the description of several candidates (including genes encoding extracellular matrix and ribosomal components, cytoskeletal proteins, apoptotic regulators, and novel tissue-specific biomarkers, our study illustrates the utility of in silico transcriptomics to identify tumor cell signatures, tumor-related genes and chromosomal regions frequently associated with aberrant expression in cancer.

  13. Sugarcane expressed sequences tags (ESTs encoding enzymes involved in lignin biosynthesis pathways

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    Ramos Rose Lucia Braz

    2001-01-01

    Full Text Available Lignins are phenolic polymers found in the secondary wall of plant conductive systems where they play an important role by reducing the permeability of the cell wall to water. Lignins are also responsible for the rigidity of the cell wall and are involved in mechanisms of resistance to pathogens. The metabolic routes and enzymes involved in synthesis of lignins have been largely characterized and representative genes that encode enzymes involved in these processes have been cloned from several plant species. The synthesis of lignins is liked to the general metabolism of the phenylpropanoids in plants, having enzymes (e.g. phenylalanine ammonia-lyase (PAL, cinnamate 4-hydroxylase (C4H and caffeic acid O-methyltransferase (COMT common to other processes as well as specific enzymes such as cinnamoyl-CoA reductase (CCR and cinnamyl alcohol dehydrogenase (CAD. Some maize and sorghum mutants, shown to have defective in CAD and/or COMT activity, are easier to digest because they have a reduced lignin content, something which has motivated different research groups to alter the lignin content and composition of model plants by genetic engineering try to improve, for example, the efficiency of paper pulping and digestibility. In the work reported in this paper, we have made an inventory of the sugarcane expressed sequence tag (EST coding for enzymes involved in lignin metabolism which are present in the sugarcane EST genome project (SUCEST database. Our analysis focused on the key enzymes ferulate-5-hydroxylase (F5H, caffeic acid O-methyltransferase (COMT, caffeoyl CoA O-methyltransferase (CCoAOMT, hydroxycinnamate CoA ligase (4CL, cinnamoyl-CoA reductase (CCR and cinnamyl alcohol dehydrogenase (CAD. The comparative analysis of these genes with those described in other species could be used as molecular markers for breeding as well as for the manipulation of lignin metabolism in sugarcane.

  14. A comprehensive assessment of the transcriptome of cork oak (Quercus suber) through EST sequencing.

    Science.gov (United States)

    Pereira-Leal, José B; Abreu, Isabel A; Alabaça, Cláudia S; Almeida, Maria Helena; Almeida, Paulo; Almeida, Tânia; Amorim, Maria Isabel; Araújo, Susana; Azevedo, Herlânder; Badia, Aleix; Batista, Dora; Bohn, Andreas; Capote, Tiago; Carrasquinho, Isabel; Chaves, Inês; Coelho, Ana Cristina; Costa, Maria Manuela Ribeiro; Costa, Rita; Cravador, Alfredo; Egas, Conceição; Faro, Carlos; Fortes, Ana M; Fortunato, Ana S; Gaspar, Maria João; Gonçalves, Sónia; Graça, José; Horta, Marília; Inácio, Vera; Leitão, José M; Lino-Neto, Teresa; Marum, Liliana; Matos, José; Mendonça, Diogo; Miguel, Andreia; Miguel, Célia M; Morais-Cecílio, Leonor; Neves, Isabel; Nóbrega, Filomena; Oliveira, Maria Margarida; Oliveira, Rute; Pais, Maria Salomé; Paiva, Jorge A; Paulo, Octávio S; Pinheiro, Miguel; Raimundo, João A P; Ramalho, José C; Ribeiro, Ana I; Ribeiro, Teresa; Rocheta, Margarida; Rodrigues, Ana Isabel; Rodrigues, José C; Saibo, Nelson J M; Santo, Tatiana E; Santos, Ana Margarida; Sá-Pereira, Paula; Sebastiana, Mónica; Simões, Fernanda; Sobral, Rómulo S; Tavares, Rui; Teixeira, Rita; Varela, Carolina; Veloso, Maria Manuela; Ricardo, Cândido P P

    2014-05-15

    Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic information. Cork oak forests are of great ecological importance and represent a major economic and social resource in Southern Europe and Northern Africa. However, global warming is threatening the cork oak forests by imposing thermal, hydric and many types of novel biotic stresses. Despite the economic and social value of the Q. suber species, few genomic resources have been developed, useful for biotechnological applications and improved forest management. We generated in excess of 7 million sequence reads, by pyrosequencing 21 normalized cDNA libraries derived from multiple Q. suber tissues and organs, developmental stages and physiological conditions. We deployed a stringent sequence processing and assembly pipeline that resulted in the identification of ~159,000 unigenes. These were annotated according to their similarity to known plant genes, to known Interpro domains, GO classes and E.C. numbers. The phylogenetic extent of this ESTs set was investigated, and we found that cork oak revealed a significant new gene space that is not covered by other model species or EST sequencing projects. The raw data, as well as the full annotated assembly, are now available to the community in a dedicated web portal at http://www.corkoakdb.org. This genomic resource represents the first trancriptome study in a cork producing species. It can be explored to develop new tools and approaches to understand stress responses and developmental processes in forest trees, as well as the molecular cascades underlying cork differentiation and disease response.

  15. Analysis of newly established EST databases reveals similarities between heart regeneration in newt and fish

    Directory of Open Access Journals (Sweden)

    Weis Patrick

    2010-01-01

    Full Text Available Abstract Background The newt Notophthalmus viridescens possesses the remarkable ability to respond to cardiac damage by formation of new myocardial tissue. Surprisingly little is known about changes in gene activities that occur during the course of regeneration. To begin to decipher the molecular processes, that underlie restoration of functional cardiac tissue, we generated an EST database from regenerating newt hearts and compared the transcriptional profile of selected candidates with genes deregulated during zebrafish heart regeneration. Results A cDNA library of 100,000 cDNA clones was generated from newt hearts 14 days after ventricular injury. Sequencing of 11520 cDNA clones resulted in 2894 assembled contigs. BLAST searches revealed 1695 sequences with potential homology to sequences from the NCBI database. BLAST searches to TrEMBL and Swiss-Prot databases assigned 1116 proteins to Gene Ontology terms. We also identified a relatively large set of 174 ORFs, which are likely to be unique for urodele amphibians. Expression analysis of newt-zebrafish homologues confirmed the deregulation of selected genes during heart regeneration. Sequences, BLAST results and GO annotations were visualized in a relational web based database followed by grouping of identified proteins into clusters of GO Terms. Comparison of data from regenerating zebrafish hearts identified biological processes, which were uniformly overrepresented during cardiac regeneration in newt and zebrafish. Conclusion We concluded that heart regeneration in newts and zebrafish led to the activation of similar sets of genes, which suggests that heart regeneration in both species might follow similar principles. The design of the newly established newt EST database allows identification of molecular pathways important for heart regeneration.

  16. Plastics and microplastics on recreational beaches in Punta del Este (Uruguay): Unseen critical residents?

    Science.gov (United States)

    Lozoya, J P; Teixeira de Mello, F; Carrizo, D; Weinstein, F; Olivera, Y; Cedrés, F; Pereira, M; Fossati, M

    2016-11-01

    Beaches are social-ecological systems that provide several services improving human well-being. However, as one of the major coastal interfaces they are subject to plastic pollution, one of the most significant global environmental threats at present. For the first time for Uruguayan beaches, this study assessed and quantified the accumulation of plastic and microplastic debris on sandy beaches of the major touristic destination Punta del Este during the austral spring of 2013. Aiming to provide valuable information for decision-making, we performed a detailed analysis of plastic debris, their eventual transport pathways to the coast (from land and sea), and the associated persistent pollutants. The results indicated that the smallest size fractions (plastic debris, and their levels did not differ from baseline values reported for similar locations. The abundance of plastic debris was significantly and positively correlated with both the presence of possible land-based sources (e.g. storm-water drains, beach bars, beach access, car parking, and roads), and dissipative beach conditions. The analysis of coastal currents suggested some potential deposition areas along Punta del Este, and particularly for resin pellets, although modeling was not conclusive. From a local management point of view, the development and use of indices that allow predicting trends in the accumulation of plastic debris would be critically useful. The time dimension (e.g. seasonal) should also be considered for this threat, being crucial for locations such as Uruguay, where the use of beaches increases significantly during the summer. This first diagnosis aims to generate scientific baseline, necessary for improved management of plastic litter on beaches and their watersheds. Copyright © 2016 Elsevier Ltd. All rights reserved.

  17. Generation of Phaseolus vulgaris ESTs and investigation of their regulation upon Uromyces appendiculatus infection

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    Nguyen Henry T

    2009-04-01

    Full Text Available Abstract Background Phaseolus vulgaris (common bean is the second most important legume crop in the world after soybean. Consequently, yield losses due to fungal infection, like Uromyces appendiculatus (bean rust, have strong consequences. Several resistant genes were identified that confer resistance to bean rust infection. However, the downstream genes and mechanisms involved in bean resistance to infection are poorly characterized. Results A subtractive bean cDNA library composed of 10,581 unisequences was constructed and enriched in sequences regulated by either bean rust race 41, a virulent strain, or race 49, an avirulent strain on cultivar Early Gallatin carrying the resistance gene Ur-4. The construction of this library allowed the identification of 6,202 new bean ESTs, significantly adding to the available sequences for this plant. Regulation of selected bean genes in response to bean rust infection was confirmed by qRT-PCR. Plant gene expression was similar for both race 41 and 49 during the first 48 hours of the infection process but varied significantly at the later time points (72–96 hours after inoculation mainly due to the presence of the Avr4 gene in the race 49 leading to a hypersensitive response in the bean plants. A biphasic pattern of gene expression was observed for several genes regulated in response to fungal infection. Conclusion The enrichment of the public database with over 6,000 bean ESTs significantly adds to the genomic resources available for this important crop plant. The analysis of these genes in response to bean rust infection provides a foundation for further studies of the mechanism of fungal disease resistance. The expression pattern of 90 bean genes upon rust infection shares several features with other legumes infected by biotrophic fungi. This finding suggests that the P. vulgaris-U. appendiculatus pathosystem could serve as a model to explore legume-rust interaction.

  18. Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR Marker Resources for Diversity Analysis of Mango (Mangifera indica L.

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    Natalie L. Dillon

    2014-01-01

    Full Text Available In this study, a collection of 24,840 expressed sequence tags (ESTs generated from five mango (Mangifera indica L. cDNA libraries was mined for EST-based simple sequence repeat (SSR markers. Over 1,000 ESTs with SSR motifs were detected from more than 24,000 EST sequences with di- and tri-nucleotide repeat motifs the most abundant. Of these, 25 EST-SSRs in genes involved in plant development, stress response, and fruit color and flavor development pathways were selected, developed into PCR markers and characterized in a population of 32 mango selections including M. indica varieties, and related Mangifera species. Twenty-four of the 25 EST-SSR markers exhibited polymorphisms, identifying a total of 86 alleles with an average of 5.38 alleles per locus, and distinguished between all Mangifera selections. Private alleles were identified for Mangifera species. These newly developed EST-SSR markers enhance the current 11 SSR mango genetic identity panel utilized by the Australian Mango Breeding Program. The current panel has been used to identify progeny and parents for selection and the application of this extended panel will further improve and help to design mango hybridization strategies for increased breeding efficiency.

  19. Ocio estético valioso en la Poética de Aristóteles

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    Mª Luisa Amigo Fernández De Arroyabe

    2015-01-01

    Full Text Available Este artículo se centra en el ocio estético valioso en Aristóteles, partiendo del análisis de la Poética y, específicamente, de la comprensión de la experiencia estética como actividad mental. Se destaca su dimensión ética, realzando su valor en el desarrollo del ser humano, así como el de la propia obra de arte, en la medida en que el poeta ha sabido construirla, encarnando en la trama los hechos y universalizándolos, al modo que le es propio. Aristóteles se refiere a la tragedia, pero por extensión esta valoración proporciona una pauta de lectura de la experiencia de ocio estético. El marco autónomo para el ámbito estético, que inaugura Aristóteles, y su valoración vinculada a la dimensión ética, a la comprensión intelectual y al placer estético proporcionan la base para sostener que el ocio estético se confirma como un horizonte enmarcado en la senda de lo valioso y adecuado para realizar la vida que le es propia al ser humano.

  20. La importancia de los costos estándar para propósitos empresariales: un estudio de caso

    OpenAIRE

    Alzate Castro, Wilmar Alexander

    2015-01-01

    Resumen: Este trabajo documenta las características, críticas, metodologías de cálculo y ventajas que tienen los costos estándar como herramienta de gestión y control en las organizaciones, a la luz de la aplicación de las buenas prácticas empresariales y los Estándares Internacionales de Información Financiera – IFRS. El trabajo contribuye en la aplicación de los diagramas de control para facilitar la selección de las variaciones que han de ser examinadas, determinar sus causas y establecer ...