WorldWideScience

Sample records for chinese shrimp fenneropenaeus

  1. Effects of different diets on the dietary attractability and selectivity of Chinese shrimp, Fenneropenaeus chinensis

    Science.gov (United States)

    Huang, Guoqiang; Dong, Shuanglin; Wang, Fang

    2005-01-01

    Attractabilities of different diets and dietary selectivity of Chinese shrimp, Fenneropenaeus chinensis were studied through behavior observation and feeding experiment, respectively. The five diets used in the experiment are: Fish Flesh (FF), Shrimp Flesh (SF), Clam Foot (CF), Polychaete Worm (PW), and Formulated Diet (FD). No significant differences of attractability exist between any two different diets when every two natural diets or all five diets are provided simultaneously. On the other hand, significant differences of attractability exist between FD and every single natural diet when they are provided simultaneously. Results of behavioral observation indicate that natural diets are more attractive than FD. In feeding experiment, Chinese shrimp has distinct selectivity on different diets. It positively selects CF and PW, negatively selects FF and SF, and excludes FD absolutely. The results of the present studies indicate that the dietary selectivity of shrimp was based not only on the attractabilities of the diets, but also on the responses such as growth and food conversion.

  2. Effects of Different Diets on the Dietary Attractability and Selectivity of Chinese Shrimp, Fenneropenaeus chinensis

    Institute of Scientific and Technical Information of China (English)

    HUANG Guoqiang; DONG Shuanglin; WANG Fang

    2005-01-01

    Attractabilities of different diets and dietary selectivity of Chinese shrimp, Fenneropenaeus chinensis were studied through behavior observation and feeding experiment, respectively. The five diets used in the experiment are: Fish Flesh (FF), Shrimp Flesh (SF), Clam Foot (CF), Polychaete Worm (PW), and Formulated Diet (FD). No significant differences of attractability exist between any two different diets when every two natural diets or all five diets are provided simultaneously. On the other hand, significant differences of attractability existbetween FD and every single natural diet when they are provided simultaneously. Results of behavioral observation indicate that natural diets are more attractive than FD.In feeding experiment, Chineseshrimp has distinct selectivity on different diets. It positively selects CF and PW, negatively selects FF and SF, and excludes FD absolutely. The results of the present studies indicate that the dietary selectivity of shrimp was based not only on the attractabilities of the diets, but also on the responses such as growth and food conversion.

  3. QTL associated with WSSV-resistance and growth-related traits in the Chinese shrimp Fenneropenaeus chinensis

    OpenAIRE

    WANG Wei-Ji; ZHANG Tian-Shi; YANG Cui-Hua; Luan, Sheng; Kong, Jie; Wang, Qing-Yin

    2008-01-01

    A virgin female Chinese shrimp Fenneropenaeus chinensis was selected from the fourth generation of a successively WSSV(White Spot Syndrome Virus) resistance population and mated with a wild male through artificial insemination to produce the F1 and F2 generation. These shrimp were challenged with WSSV and the F2 generation were collected and scored for WSSV resistance, standard length, total body length and body weight. AFLP (Amplified Fragment Length Polymorphism) markers were used to constr...

  4. Purification and identification of a clotting protein from the hemolymph of Chinese shrimp ( Fenneropenaeus chinensis)

    Science.gov (United States)

    Wang, Baojie; Peng, Hongni; Liu, Mei; Jiang, Keyong; Zhang, Guofan; Wang, Lei

    2013-09-01

    The clotting protein (CP) plays important and diverse roles in crustaceans, such as coagulation and lipid transportation. A clotting protein was purified from the hemolymph of Chinese shrimp Fenneropenaeus chinensis (named as Fc-CP) with Q sepharose HP anion-exchange chromatography and phenyl sepharose HP hydrophobic interaction chromatography. Fc-CP was able to form stable clots in vitro in the presence of hemocyte lysate and Ca2+, suggesting that the clotting reaction is catalyzed by a Ca2+-dependent transglutaminase in shrimp hemocytes. The molecular mass of Fc-CP was 380 kDa under non-reducing conditions and 190 kDa under reducing conditions as was determined with SDS-PAGE. CP exists as disulfide-linked homodimers and oligomers. The N-terminal amino acid sequence of Fc-CP was identical to that of shrimps including Penaeus monodon, Farfantepenaeus paulensis and Litopenaeus vannamei; and similar to that of other decapods. The purified Fc-CP was digested with trypsin and verified on an ABI 4700 matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) mass spectrometry. Our results will aid to better understanding the coagulation mechanism of shrimp hemolymph.

  5. Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis

    Institute of Scientific and Technical Information of China (English)

    LIU Fengsong; LI Fuhua; XIANG Jianhai; DONG Bo; LIU Yichen; ZHANG Xiaojun; ZHANG Liusuo

    2008-01-01

    A new member of antimicrobial protein genes of the Crustin family was cloned from haemocytes of the Chinese shrimp Fennero-penaeus chinensis by 3'and 5' RACE.The full-length cDNA of Crustin-like gene contains a 390 bp open reading frame,encoding 130 amino acids.The deduced peptide contains a putative signal peptide of 17 amino acids and mature peptide of 113 amino acids.The molecular mass of the deduced mature peptide is 12.3 ku.It is highly cationic with a theoretical isoelectric point of 8.5.The deduced amino acids sequence of this Crustin showed high homology with those of Penaeus (Litopenaeus) setferus.Northern blotting showed that the cloned Crustin gene was mainly expressed in haemocytes,gill,intestine,and RNA in situ hy-bridization indicated that the Crustin gene was constitutively expressed exclusively in haemocytes of these tissues.Capillary elee-trephoresis RT-PCR analysis showed that Crustin was up-regulated dramatically from 12 to 48 h after a brief decrease of mRNA during first 6 h in response to microbe infection.The level of Crustin mRNA began to restore at 72 h post-challenge.This indica-ted that Crustin gene might play an important role when shrimps are infected by bacterial pathogen.

  6. Establishment of microsatellite-based triplex PCR for parentage analysis of Chinese shrimp Fenneropenaeus chinensis

    Institute of Scientific and Technical Information of China (English)

    GAO Huan; KONG Jie; LIU Ping; MENG Xianhong; LUAN Sheng; ZHANG Tianshi

    2007-01-01

    Through exploring the microsatellite primers from the random genome sequences of Chinese shrimp (Fenneropenaeus chinensis), some microsatellite primers were obtained with rich polymorphic genetic information, and a triplex PCR was established using three primers (RS1101, RS0683 and H081 primers). By adjusting the final concentration of Mg2+, dNTP and primers, and using a touch-town PCR program, the optimum amplification parameters of PCR system were obtained, which could successfully amplify the three primers in a PCR reaction. In the denatured PAGE gel, the amplified DNA fragments of three primers RS1 101,RS0683 and H081 could be easily identified each other. For the triplex PCR system, the PPE (probabilities of paternity exclusion) is 0.967 9,and the DP (discrimination power) is 0.999 327.Using the triplex PCR to test ten individuals of a parentage and their parents, an individual was excluded from the parentage in all of the three microsatellite loci, which might be mixed into the parentage for some unknown reason such as factitious misplay. The triplex PCR will be of great practical value in identifying the parentages of F. chinensis.

  7. A Successful Microbound Diet for the Larval Culture of Chinese Shrimp Fenneropenaeus chinensis

    Institute of Scientific and Technical Information of China (English)

    WANG Xinxia; MAI Kangsen

    2005-01-01

    A 13-day feeding trial was carried out to evaluate the effectiveness of a microbound diet for rearing the larvae of Chinese shrimp Fenneropenaeus chinensis in comparison with the live foods that consist of Isochrysis galbana, Chlorella vulgaris, Tetraselmis chuii, rotifer (Brachionus plicatilis) and Artemia sp. Larvae of 0 to 13d post-hatch (dph) were reared in a temperature-controlled semi-open culture system and stocked at a density of 100 larvae L-1 in tanks, each containing 50 L sterilized seawater with salinity 30-32. Larvae were manually fed either the live foods or the microbound diet 6 times per day. At 13 dph, the growth of the larvae fed on the microbound diet was approximately 84% of that fed on the live foods.The survival rate of the larvae fed on the microbound diet was 44.29% at 13 dph, which was not significantly different from that of larvae fed on live foods (63.55%). The body length and development index (DI) of the larvae fed on the microbound diet were always lower than those of larvae fed on live foods. However, the differences reached significant levels only at 11and 13dph (P<0.05). The mean dry weight loss of the microbound diet was 9.2% after 90min immersion in seawater, indicating that this diet has a good water stability. The microbound diet contains 52.23% crude protein and 10.27% lipid and is easy to prepare. These characteristics of the diet suggest good potentials for its successful use in the larviculture of other penaeid and fish species.

  8. A successful microbound diet for the larval culture of Chinese shrimp Fenneropenaeus chinensis

    Science.gov (United States)

    Wang, Xinxia; Mai, Kangsen

    2005-07-01

    A 13-day feeding trial was carried out to evaluate the effectiveness of a microbound diet for rearing the larvae of Chinese shrimp Fenneropenaeus chinensis in comparison with the live foods that consist of Isochrysis galbana, Chlorella vulgaris, Tetraselmis chuii, rotifer (Brachionus plicatilis) and Artemia sp. Larvae of 0 to 13d post-hatch (dph) were reared in a temperature-controlled semi-open culture system and stocked at a density of 100 larvae L-1 in tanks, each containing 50 L sterilized seawater with salinity 30 32. Larvae were manually fed either the live foods or the microbound diet 6 times per day. At 13dph, the growth of the larvae fed on the microbound diet was approximately 84% of that fed on the live foods. The survival rate of the larvae fed on the microbound diet was 44.29% at 13 dph, which was not significantly different from that of larvae fed on live foods (63.55% ). The body length and development index (DI) of the larvae fed on the microbound diet were always lower than those of larvae fed on live foods. However, the differences reached significant levels only at 11 and 13dph (P<0.05). The mean dry weight loss of the microbound diet was 9.2% after 90min immersion in seawater, indicating that this diet has a good water stability. The microbound diet contains 5223% crude protein and 10.27% lipid and is easy to prepare. These characteristics of the diet suggest good potentials for its successful use in the larviculture of other penaeid and fish species.

  9. QTL associated with WSSV-resistance and growth-related traits in the Chinese shrimp Fenneropenaeus chinensis

    Directory of Open Access Journals (Sweden)

    WANG Wei-Ji

    2008-12-01

    Full Text Available A virgin female Chinese shrimp Fenneropenaeus chinensis was selected from the fourth generation of a successively WSSV(White Spot Syndrome Virus resistance population and mated with a wild male through artificial insemination to produce the F1 and F2 generation. These shrimp were challenged with WSSV and the F2 generation were collected and scored for WSSV resistance, standard length, total body length and body weight. AFLP (Amplified Fragment Length Polymorphism markers were used to construct a linkage map of F.chinensis based on this F2 family. The MAPMAKER/QTL1.1 software was applied in QTL (Quantitative Traits Loci mapping of standard length, total body length, weight and WSSV-resistant of F.chinensis based on interval mapping method (IM. At the criterion of LOD (Limit of Detection >2.0, one QTL associated with standard length, two with total body length, two with body weight and two with WSSV-resistance were detected. These QTL were distributed on three linkage groups. The variance-explained value of QTLs were between 26.6% and 66.9%. Three QTL for body weight, total body length and WSSV-resistance were detected on LG11, two QTLs for standard length and total body length shared a same LG (Linkage Group, and two QTL of WSSV-resistance and body weight were distributed on another LG. These results suggested that the WSSV-resistance and the body shape traits of F.chinensis are correlated[Acta Zoologica Sinica 54(6: 1075 – 1081, 2008].

  10. Cloning,characterization,and expression analysis of the DEAD-box family genes,Fc-vasa and Fc-PL10a,in Chinese shrimp (Fenneropenaeus chinensis)

    Institute of Scientific and Technical Information of China (English)

    周倩如; 邵明瑜; 秦贞奎; 康庆浩; 张志峰

    2010-01-01

    RNA helicases of the DEAD-box and related families are involved in various cellular processes including DNA replication,DNA repair,and RNA processing.However,the function of DEAD-box proteins in aquaculture species is poorly understood at molecular level.We obtained the full-length cDNA sequences of two genes encoding helicase-related proteins,Fc-vasa and Fc-PL10a,from the testes of Chinese shrimp,Fenneropenaeus chinensis.The two predicted amino acid sequences contain all the conserved motifs characterized ...

  11. Ecological effects of co-culturing the sea cucumber Apostichopus japonicus with the Chinese white shrimp Fenneropenaeus chinensis in an earthen pond

    Science.gov (United States)

    Zhou, Shun; Ren, Yichao; Pearce, Christopher M.; Dong, Shuanglin; Tian, Xiangli; Gao, Qinfeng; Wang, Fang

    2016-03-01

    Using net enclosures in an earthen pond, we established three culture treatments with the sea cucumber Apostichopus japonicus and the Chinese white shrimp Fenneropenaeus chinensis: monoculture of sea cucumbers (C), monoculture of shrimp (S), and co-culture of the two species (CS). We measured levels of suspended particulate matter in the water column; total organic matter, total organic carbon, total nitrogen, and carbon/nitrogen ratios in both settling particles and the sediment; and chlorophyll a levels in the sediment. We then compared these variables between the three treatments. We also examined growth, survival, and yield of the two species in the different treatments. From June to September, the mean monthly suspended particulate matter sedimentation rates in the CS and S treatments were significantly (Pculture were all significantly greater than those of sea cucumbers in monoculture. There were no significant differences among any of these variables for shrimp reared in the two systems. The bioturbation of the sediment and fecal production of the shrimp likely supplied natural food for the sea cucumbers. Co-culture of the two species is a viable option for increasing yield per unit area, maximizing use of the water body, and diversifying crop production.

  12. Molecular cloning and characterisation of a pattern recognition protein, lipopolysaccharide and beta-1,3-glucan binding protein (LGBP) from Chinese shrimp Fenneropenaeus chinensis.

    Science.gov (United States)

    Liu, Fengsong; Li, Fuhua; Dong, Bo; Wang, Xiaomei; Xiang, Jianhai

    2009-03-01

    A pattern recognition protein (PRP), lipopolysaccharide and beta-1,3-glucan binding protein (LGBP) cDNA was cloned from the haemocyte of Chinese shrimp Fenneropenaeus chinensis by the techniques of homology cloning and RACE. Analysis of nucleotide sequence revealed that the full-length cDNA of 1,275 bp has an open reading frame of 1,098 bp encoding a protein of 366 amino acids including a 17 amino acid signal peptide. Sequence comparison of the deduced amino acid sequence of F. chinensis LGBP showed a high identity of 94%, 90%, 87%, 72% and 63% with Penaeus monodon BGBP, Litopenaeus stylirostris LGBP, Marsupenaeu japonicus BGBP, Homarus gammarus BGBP and Pacifastacus leniusculus LGBP, respectively. The calculated molecular mass of the mature protein is 39,857 Da with a deduced pI of 4.39. Two putative integrin binding motifs, RGD (Arg-Gly-Asp) and a potential recognition motif for beta-1,3-linkage of polysaccharides were observed in LGBP sequence. RT-PCR analysis showed that LGBP gene expresses in haemocyte and hepatopancreas only, but not in other tissues. Capillary electrophoresis RT-PCR method was used to quantify the variation of mRNA transcription level during artificial infection with heat-killed Vibrio anguillarum and Staphylococcus aureusin. A significant enhancement of LGBP transcription was appeared at 6 h post-injection in response to bacterial infection. These results have provided useful information to understand the function of LGBP in shrimp. PMID:18163220

  13. Expression, Purification and Activity Assay of the Recombinant Protein of Catechol-O-Methyltransferase from Chinese White Shrimp (Fenneropenaeus chinensis

    Directory of Open Access Journals (Sweden)

    Dian-Xiang Li

    2010-01-01

    Full Text Available Problem statement: We have previously cloned a gene of Chinese white shrimp Catechol O-Methyltransferase (designated Fc-COMT and characterized the gene expression pattern. In this study, expression and purification as well as activity assay of the recombinant Fc-COMT was further conducted. Approach: Using pET-30a (+ as a prokaryotic expression vector, the recombinant Fc- COMT was expressed in the supernatant of Escherichia coli lysate and easily purified by His-Bind resin chromatography. SDS-PAGE analysis showed that the molecular mass of recombinant Fc-COMT was approximately 30,000 Da, in good agreement with the software-predicted molecular weight. The enzymatic activity of recombinant Fc-COMT was tested using Dihydroxybenzoic Acid (DHBAc as a substrate. Results: The methyl products of DHBAc, Vanillic Acid (VA and Isovanillic Acid (IVA, were detected in the enzymatic reaction mixture with recombinant Fc-COMT by High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS. Conclusion: The recombinant Fc-COMT has catalytic activity of transferring methyl group from S-Adenosyl-L-Methionine (SAM to the 3’ hydroxyl or 4’ hydroxyl group of benzyl ring of DHBAc.

  14. Peptidomic analysis of Chinese shrimp ( Fenneropenaeus chinensis) hemolymph by magnetic bead-based MALDI-TOF MS

    Science.gov (United States)

    Wang, Baojie; Liu, Mei; Jiang, Keyong; Zhang, Guofan; Wang, Lei

    2013-03-01

    Peptides in shrimp hemolymph play an important role in the innate immune response. Analysis of hemolymph will help to detect and identify potential novel biomarkers of microbial infection. We used magnetic bead-based purification (ClinProt system) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) to characterize shrimp hemolymph peptides. Shrimp serum and plasma were used as the source of samples for comparative analysis, and it was found that serum was more suitable for shrimp hemolymph peptidomic analysis. To screen potential specific biomarkers in serum of immune-challenged shrimps, we applied magnetic bead-based MALDI-TOF MS to serum samples from 10 immune-challenged and 10 healthy shrimps. The spectra were analyzed using FlexAnalysis 3.0 and ClinProTools 2.1 software. Thirteen peptide peaks significantly different between the two groups were selected as candidate biomarkers of lipopolysaccharide (LPS)-infection. The diagnostic model established by genetic algorithm using five of these peaks was able to discriminate LPS-challenged shrimps from healthy control shrimps with a sensitivity of 90% and a specificity of 100%. Our approach in MALDITOF MS-based peptidomics is a powerful tool for screening bioactive peptides or biomarkers derived from hemolymph, and will help to enable a better understanding of the innate immune response of shrimps.

  15. Effects of salinity fluctuation frequency on the growth, molting rate and hemolymph 20-hydroxyecdysone concentration in Juvenile Chinese shrimp, Fenneropenaeus chinensis

    Science.gov (United States)

    Ding, Sen; Wang, Fang; Sun, Hao; Dong, Shuanglin

    2009-09-01

    This experiment was conducted to examine the effects of salinity fluctuation frequency on the growth, molting rate and hemolymph 20-hydroxyecdysone (20-HE) concentration for juvenile Fenneropenaeus chinensis with an initial wet body weight of 1.115 g±0.012 g. The salinity of the control treatment (represented by D0) was 28 throughout the experiment, whereas treatments D2, D4, D6 and D8 were subjected to different salinity fluctuation frequencies of 2, 4, 6 and 8 d, respectively. The range of salinity fluctuation was 4. The salinity in treatments D2, D4, D6 and D8 was kept at 28 for 2, 4, 6 and 8 d, respectively; each original salinity was decreased abruptly to salinity 24, which first lasted for another 2 d and was then raised to its initial value 28. This constituted a salinity fluctuation cycle and afterwards the cycle repeated. It was found that: 1) The weight gain of shrimp in treatment D4 was 202.9% and significantly higher than that in treatment D0 ( Phemolymph 20-HE concentration of shrimps in all treatments was at low levels and increased first gradually during the inter-molt period and then increased sharply. It reached a peak value during the pre-molt stage, and then decreased abruptly during the post-molt stage when the lowest level occurred. 3) During the post-molt stage, the hemolymph 20-HE concentration in treatment D2 was 7.47 pg μL-1 and significantly higher than that in treatment D0 ( Phemolymph 20-HE concentration among all five treatments during the inter-molt stage ( P>0.05). During the pre-molt stage, the hemolymph 20-HE concentration in treatment D6 was significantly higher than that in treatment D0 ( Phemolymph 20-HE concentration in treatment D2 was significantly lower than that in other treatments ( Phemolymph 20-HE concentration at the pre-molt stage.

  16. 中国对虾(Fenneropenaeus chinensis)多性状复合育种方案的遗传和经济评估%Genetic evaluation and investment appraisal of the multi-trait selection breeding program in Chinese shrimp Fenneropenaeus chinensis

    Institute of Scientific and Technical Information of China (English)

    栾生; 金武; 孔杰; 罗坤; 孟宪红; 张天时; 王清印

    2013-01-01

    设计中国对虾Fenneropenaeus chinensis多性状复合育种方案,模拟选择20个世代,预测和评估目标性状(收获体重(BW),存活率(SR)和饲料摄入量(FI))的遗传进展及经济效益.利用选择指数理论,估计目标性状的选择反应和遗传进展;通过三级金字塔传递系统(核心群、扩繁群和生产群)放大遗传进展并计算其经济效益;对影响利润(RP)和效益成本比率(BCR)的生物学参数(遗传力、育种目标是否包括FI),经济学参数(对虾价格、饲料价格、贴现率、初投资、年费用)和运行参数(首次回报年份、扩繁效率)进行敏感性分析.结果表明,在基础参数值下,BW、SR和FI每个世代的选择反应分别为0.81g、1.41%和1.30 g;以1 000尾虾为单位计算,BW、SR和FI的经济加权值分别为32元,20元和-8元,育种目标的遗传进展为43.69元,育种方案执行20年产生的RP和BCR分别为8 660.99万元和14.34.敏感性分析显示,生物学参数中,目标性状遗传力变化对RP和BCR影响程度中等,遗传力值越高,RP和BCR越大;将FI经济加权值设为0,育种方案的RP和BCR明显高于基础参数.经济学参数中,对虾价格的变化对RP和BCR影响较大;饲料价格、贴现率和年费用的变化对RP和BCR影响较小,但初投资变化对BCR影响较大.运行参数中,首次回报年份越晚,RP和BCR越低.扩繁效率是影响RP和BCR的最重要因素.高扩繁效率下,生产群规模扩大1 736.15%,RP和BCR分别提高1 866.92%和1 736.75%.%The genetic gain and economic benefit from a multi-trait breeding scheme with Chinese shrimp (Fennero-penaeus chinensis) were calculated after performing 20 selections. The breeding object was made up of harvest body weight (BW), survival rate (SR) and feed intake (FI), but the trait FI was not included in the selection index. Selection response and genetic gain of the breeding object were estimated based on selection index theory. Economic benefit

  17. The Use of Eugenol as an Anesthetic in Transportation of With Indian Shrimp (Fenneropenaeus indicus) Post Larvae

    OpenAIRE

    Akbari, Sohrab; Khoshnod, Mohammad J.; Rajaian, Hamid; Afsharnasab, Mohammad

    2010-01-01

    The safe margin of eugenol for sedation of the healthy post larvae (PL) of white Indian shrimp (Fenneropenaeus indicus) was determined by estimating the median lethal concentration of eugenol during 24 hours (24hLC50). Sedation concentration of eugenol was obtained experimentally by considering behavioral factors of PLs such as response to indirect stimuli and changes in water quality parameters. Applying the estimated concentrations of eugenol for 12 hours transportation of PLs, biometric pa...

  18. Heat-shock protein 70 expression in shrimp Fenneropenaeus chinensis during thermal and immune-challenged stress

    Institute of Scientific and Technical Information of China (English)

    GUO Zhenyu; JIAO Chuanzhen; XIANG Jianhai

    2004-01-01

    Using western immunoblotting, we obtained heat-shock protein 70 (HSP70) induction data and distribution in different tissues from shrimp Fenneropenaeus chinensis during thermal and immune-challenged stresses. This is probably the first report of the effects of various stressors on the expression of HSP70 in shrimp. HSP70 was prominently induced in hepatopancreas and gills, but not in muscle, eyestalk and hemolymph, when the shrimp were exposed to heat shock and Vibrio anguillavium-challenged stresses. Cold shock and WSSV treatment had no significant effects on the levels of HSP70 expression in all tissues examined. HSP70 induction was greatest after 2 h exposure to heat shock stress, which was elevated after acute heat shock exposure of 10℃ above ambient temperature.

  19. The complete mitochondrial genome of banana shrimp Fenneropenaeus merguiensis with phylogenetic consideration.

    Science.gov (United States)

    Zhang, Dianchang; Huang, Jianhua; Zhou, Falin; Gong, Fahui; Jiang, Shigui

    2016-07-01

    The complete mitochondrial genome sequence of Fenneropenaeus merguiensis was determined by shotgun assembly method. The complete mitochondrial DNA sequence is a circular molecule with 16,023 bp in length including 13 protein-coding genes, 22 transfer RNA genes, 2 rRNA genes and a control region. The gene arrangements are consistent with the pan crustacean ground pattern. The molecular analyses provided robust evidence for the monophyly of Fenneropenaeus, but Litopenaeus was not monophyletic. Phylogenetic analyses robustly supported the fact that genus Penaeus s.l. contains the two lineages: Marsupenaeus and Penaeus s.s+ Fenneropenaeus + Litopenaeus + Farfantepenaeus. PMID:26053939

  20. A Study on the Contribution of Different Food Sources to Shrimp Growth in an Intensive Fenneropenaeus chinensis Pond

    Institute of Scientific and Technical Information of China (English)

    SU Yuepeng; MA Shen; TIAN Xiangli; DONG Shuanglin

    2008-01-01

    Stable isotope methods can be used to determine the food sources and prey items of aquatic organisms accurately andreliably.This study examined the relative contribution of artificial foods (the formulated feed and Artemia) and natural foods toshrimp growth in an intensive Fenneropenaeus chinensis pond by using carbon and nitrogen stable isotopes.The results showed that the nutrition utilization efficiency of the harvested shrimp was low,only 33.18% of feed nitrogen and 21.73% of feed carbon beingconverted to shrimp flesh.Our stable isotope results showed that the shrimp obtained nutrition for maximum growth from artificialfoods,whose contribution was 93.5%,with the remaining attributed to the natural foods.However,there was 0.94 t harvested shrimpderived from natural foods (the rest of 13.56t harvested shrimp derived from artificial foods) in lha intensive pond with a shrimpproduction of 14.50 tha-1.Therefore,unit area shrimp production can be increased by increasing the contribution proportion of natu-ral foods in intensive shrimp farming.

  1. Molecular Characterization of a Newly Identified Subfamily Member of Penaeidin from two Penaeid Shrimps, Fenneropenaeus indicus and Metapenaeus monoceros.

    Science.gov (United States)

    Afsal, V V; Antony, Swapna P; Philip, Rosamma; Bright Singh, I S

    2016-03-01

    Penaeidins are a major group of antimicrobial peptides found in penaeid shrimps. This study reports a new isoform of penaeidin from the hemocytes of Indian white shrimp, Fenneropenaeus indicus (Fi-PEN, JX657680), and the pink shrimp, Metapenaeus monoceros (Mm-PEN, KF275674). Mm-PEN is also the first antimicrobial peptide to be identified from M. monoceros. The complete coding sequences of the newly identified Fi-PEN and Mm-PEN consisted of an ORF of 338 bp encoding 71 amino acids with a predicted molecular weight of 5.66 kDa and a pI of 9.38. The penaeidins had its characteristic signal peptide region (19 amino acids), which was followed by a mature peptide with a proline-rich domain (24 amino acids) at the N-terminal region and a cysteine-rich domain (28 amino acids) at the C-terminal region, designating it to penaeidin-3 subgroup. Structural analysis revealed an alpha-helix in its secondary structure and an extended structure at the proline-rich domain. The newly identified penaeidin isoform showed maximum similarity of 63 % to a penaeidin-3 isoform of P. monodon, which further proves it to be a new isoform. Phylogenetic analysis showed that it possessed similar evolutionary status like other penaeidins, which has subsequently diverged at different phases of evolution. The wide distribution of penaeidins in penaeid shrimps indicates the importance of these AMPs in the innate immunity. PMID:26607699

  2. The genetic diversity and differentiation of shrimp Fenneropenaeus chinensis in the Yellow Sea revealed by polymorphism in control region of mitochondrial DNA

    Directory of Open Access Journals (Sweden)

    L Wang

    2014-11-01

    Full Text Available Chinese white shrimp Fenneropenaeus chinensis is a commercially important species in northern China and Korea. In the present study, the genetic diversity of five populations collected from Qingdao (QD, Rizhao (RZ of China, and Narodo Island (KN, Taean (KT, Yeongguang (KY of Korea in the Yellow Sea was investigated using the mitochondrial control region (CR. The length of the amplified partial mitochondrial control region (mtCR ranged from 600 to 622 bp, and the sequence variations were distributed among 13 polymorphic sites. The pattern of nucleotide substitution was biased in favour of transitions over transversions in variable sites, including 12 transitions (si, 4 A↔G and 8 T↔C changes and only one was transversion (sv, 1 T↔G changes. Altogether, 24 unique haplotypes were identified from five populations in Yellow Sea. The overall haplotype diversity and nucleotide diversity were 0.368 - 0.421 and 0.052 - 0.079, respectively, and the lowest genetic diversity was found in QD population. There was no differentiation between the two Chinese populations (FST = 0.039. Within the Korean populations, there was a slight differentiation (FST = 0.075, p < 0.05 between KN and KT. The relative bigger differentiation was shown between RZ and KN population (FST = 0.170, p < 0.05. The relative further genetic distance was shown between RZ and KN population as well as between QD and KN population, while the relative closer genetic distance was shown between KT and KY, and between KT and RZ population. The low variability in the mitochondrial control region among F. chinensis in the Yellow Sea indicated the low genetic diversity in comparison to other shrimp species. The results suggested a slight population differentiation among F. chinensis populations. Such information will assist in sustainable use, management, and conservation of the species

  3. Effects of different levels of live food replacement with microdiet on growth factors, survival and resistance to salinity stress of Indian white shrimp post-larvae (Fenneropenaeus indicus)

    OpenAIRE

    Seyed Hossein Hoseinifar; Parviz Zare

    2013-01-01

    In this study, partial and complete replacement of live food (Artemia nauplii) with a microdiet was investigated in post-larval Indian white shrimp, Fenneropenaeus indicus. Post-larvae (PL1) were stocked into nine 20-L vessels (5-L water volume) at a density of 50 L-1. Shrimp were fed six times a day over 10 days. At the end of the feeding period, there was no significant difference in quality index (stress-test survival) among treatments. Statistical analysis of results showed that partial o...

  4. Nitrogen and Phosphorus Budget of a Polyculture System of Sea Cucumber (Apostichopus japonicus), Jellyfish (Rhopilema esculenta) and Shrimp (Fenneropenaeus chinensis)

    Institute of Scientific and Technical Information of China (English)

    LI Junwei; DONG Shuanglin; GAO Qinfeng; ZHU Changbo

    2014-01-01

    The nitrogen (N) and phosphorus (P) budget and the ecological efficiency of a polyculture system of sea cucumber (Apostichopus japonicus), jellyfish (Rhopilema esculenta) and shrimp (Fenneropenaeus chinensis) were studied in a cofferdam, 120.2 ha in size. The nutrients were supplied by spring tide inflow. In total, 139600 kg N yr-1 and 9730 kg P yr-1 input to the system;while 118900 kg N yr-1 and 2840 kg P yr-1 outflowed from the system concurrently, thus the outflow was 85.7%(N) and 29.2%(P) of inflow. The production of N and P was 889.5 kg yr-1 and 49.28 kg yr-1 (sea cucumber) and 204 kg yr-1 and 18.03 kg yr-1 (jellyfish and shrimp), respectively. The utilization rate of N and P by polycultured animals was 7.8‰and 6.9‰, respectively, 21.9%and 38%higher than that of monocultured sea cucumber. Our results indicated that the polyculture system was an efficient culture system of animals and a remediation system of coastal environment as well;it scavenged 14.3%and 70.8%of N and P, respectively. Such an ecological efficiency may be improved further by increasing either the stocking density or the size of sea cucumber or both.

  5. Nitrogen and phosphorus budget of a polyculture system of sea cucumber ( Apostichopus japonicus), jellyfish ( Rhopilema esculenta) and shrimp ( Fenneropenaeus chinensis)

    Science.gov (United States)

    Li, Junwei; Dong, Shuanglin; Gao, Qinfeng; Zhu, Changbo

    2014-06-01

    The nitrogen (N) and phosphorus (P) budget and the ecological efficiency of a polyculture system of sea cucumber ( Apostichopus japonicus), jellyfish ( Rhopilema esculenta) and shrimp ( Fenneropenaeus chinensis) were studied in a cofferdam, 120.2 ha in size. The nutrients were supplied by spring tide inflow. In total, 139600 kg N yr-1 and 9730 kg P yr-1 input to the system; while 118900 kg N yr-1 and 2840 kg P yr-1 outflowed from the system concurrently, thus the outflow was 85.7% (N) and 29.2% (P) of inflow. The production of N and P was 889.5 kg yr-1 and 49.28 kg yr-1 (sea cucumber) and 204 kg yr-1 and 18.03 kg yr-1 (jellyfish and shrimp), respectively. The utilization rate of N and P by polycultured animals was 7.8‰ and 6.9‰, respectively, 21.9% and 38% higher than that of monocultured sea cucumber. Our results indicated that the polyculture system was an efficient culture system of animals and a remediation system of coastal environment as well; it scavenged 14.3% and 70.8% of N and P, respectively. Such an ecological efficiency may be improved further by increasing either the stocking density or the size of sea cucumber or both.

  6. A genetic linkage map of marine shrimp Penaeus ( Fenneropenaeus) chinensis based on AFLP, SSR, and RAPD markers

    Science.gov (United States)

    Liu, Bo; Wang, Qingyin; Li, Jian; Liu, Ping; He, Yuying

    2010-07-01

    The Chinese shrimp Penaeus ( Fenneropaeneus) chinensis is an important species in marine fishery and aquaculture in China. A female Chinese shrimp Penaeus ( Fenneropaeneus) chinensis was captured from west coast of the Korean peninsula and mated with a “Yellow Sea No. 1” male to produce the first filial generation (F1) 100 F2 full-sib progeny from brother-sister crosses between F1 families was used for the mapping study. A genetic linkage map of the Chinese shrimp was constructed, based on 354 markers, including 300 amplified fragment length polymorphism (AFLP) markers, 42 microsatellite (SSR) markers, and 12 randomly amplified polymorphism (RAPD) markers. Forty-seven linkage groups (LGs) were identified. The total map length was 4 580.5 cM, with an average spacing of 11.3 cM, covering 75.8% of the estimated genome size. The construction of this genetic linkage map was part of a genetic breeding program. This linkage map will contribute to the discovery of genes and quantitative trait loci (QTLs) in Chinese shrimp.

  7. Assessment of antimicrobial activity of c-type lysozyme from Indian shrimp Fenneropenaeus indicus

    Directory of Open Access Journals (Sweden)

    Viswanathan Karthik

    2014-10-01

    Full Text Available Objective: To assess the multitudinal antimicrobial effects of recombinant lysozyme from Fenneropenaeus indicus (rFi-Lyz in comparison with commercially available recombinant hen egg white lysozyme (rHEWL. Methods: Antimicrobial activity of the recombinant rFi-Lyz using several Gram positive, Gram negative bacteria and fungi in comparison with rHEWL has been evaluated. rFi-Lyz was expressed and purified using Ni2+ affinity chromatography. The effect of rFi-Lyz in the growth of yeast Candida krusei, plant molds Rhizoctonia solani and Fusarium solani was assessed by well diffusion assay in petri plates with potato dextrose agar. Results: rFi-Lyz exhibited high inhibitory activity on Gram positive bacteria such as Staphylococcus aureus and Bacillus subtilis. Among various Gram negative bacteria tested Klebsiella pneumoniae exhibited the highest inhibition followed by Pseudomonas aeruginosa and Shigella dysenteriae. rFi-Lyz also exhibited significant inhibition on two marine pathogens Aeromonas veronii and Vibrio alginolyticus. Among the various fungal strains tested, rFi-Lyz inhibited the growth of budding yeast Candida krusei significantly. Further the growth of two other plants fungus Rhizoctonia solani and Fusarium oxysporum were retarded by rFi-Lyz in the plate inhibition assay. Conclusions: rFi-Lyz exhibits a broad spectrum of antimicrobial activity like a natural antibiotic on various pathogenic bacteria and fungal strains.

  8. Assessment of antimicrobial activity of c-type lysozyme from Indian shrimp Fenneropenaeus indicus

    Institute of Scientific and Technical Information of China (English)

    Viswanathan Karthik; Thomas Ancy; Dharmaraj Ramkumar; Narayanasamy Mathivanan; Rangarajan Badri Narayanan

    2014-01-01

    Objective: To assess the multitudinal antimicrobial effects of recombinant lysozyme fromFenneropenaeus indicus (rFi-Lyz) in comparison with commercially available recombinant hen egg white lysozyme (rHEWL).Methods:Antimicrobial activity of the recombinant rFi-Lyz using several Gram positive, Gram negative bacteria and fungi in comparison with rHEWL has been evaluated. rFi-Lyz was expressed and purified using Ni2+ affinity chromatography. The effect of rFi-Lyz in the growth of yeast Candida krusei, plant molds Rhizoctonia solani and Fusarium solani was assessed by well diffusion assay in petri plates with potato dextrose agar.Results: rFi-Lyz exhibited high inhibitory activity on Gram positive bacteria such as Staphylococcus aureus and Bacillus subtilis. Among various Gram negative bacteria testedKlebsiella pneumoniae exhibited the highest inhibition followed by Pseudomonas aeruginosa and Shigella dysenteriae. rFi-Lyz also exhibited significant inhibition on two marine pathogens Aeromonas veronii and Vibrio alginolyticus. Among the various fungal strains tested, rFi-Lyz inhibited the growth of budding yeast Candida krusei significantly. Further the growth of two other plants fungus Rhizoctonia solani and Fusarium oxysporum were retarded by rFi-Lyz in the plate inhibition assay.Conclusions: rFi-Lyz exhibits a broad spectrum of antimicrobial activity like a natural antibiotic on various pathogenic bacteria and fungal strains.

  9. Genetic parameters for cold tolerance and body weight of Chinese fleshy prawn,Fenneropenaeus chinensis

    Institute of Scientific and Technical Information of China (English)

    LI Wenjia; LUAN Sheng; LUO Kun; SUI Juan; LU Xia; WANG Qingyin; KONG Jie

    2016-01-01

    The inability ofFenneropenaeus chinensis to tolerate low temperatures is of major economic concern in temperate climates, as it reduces their growing season and leads to over-winter mortality. In this study, the heritability of body weight under low grow-out temperature and cold tolerance inF. chinensis were first investigated and estimated using 88 ful-sib families, which might provide crucial information in Chinese fleshy prawn breeding programs. The heritability for body weight under suitable and low temperature ofF. chinensis were both moderate (0.158 0±0.307 5 and 0.132 0±0.026 9 respectively); the large coefficient of variation (approximately 21%) and moderate estimate of heritability for body weight indicated substantial potential for selective breeding. The heritability estimate for cold tolerance was low (0.019 2±0.023 5), and showed no significant differences from zero (P>0.05). A weak genetic correlation between cold tolerance and body weight was also estimated in the present study, also showing no significant differences from zero (P>0.05). Thus, more research needs to be conducted on the more accurate heritability estimate of cold tolerance and genetic correlations between traits inF. chinensis to further improve the achievement of breeding goals.

  10. Molecular cloning of peroxinectin gene and its expression in response to peptidoglycan and Vibrio harveyi in Indian white shrimp Fenneropenaeus indicus.

    Science.gov (United States)

    Shanthi, Sathappan; Manju, Sivalingam; Rajakumaran, Perumal; Vaseeharan, Baskaralingam

    2014-12-01

    The cDNA sequence of peroxinectin was obtained from the haemocytes of Indian white shrimp Fenneropenaeus indicus using RT-PCR and RACE. Fenneropenaeus indicus peroxinectin (Fi-Pxn) sequence has an open reading frame (ORF) of 2415 bp encoding a protein of 804 amino acids with 21 residues signal sequence. The mature protein has molecular mass of 89.8 kDa with an estimated pI of 8.6. Two putative integrin-binding motifs, RGD and KGD, were observed at the basic N-terminal and C-terminal part of the mature aminoacid sequence. Fi-Pxn nucleotide sequence comparison showed high homology to mud crab Scylla serrata (89%) and to various vertebrate and invertebrate species. qRT-PCR showed peroxinectin mRNA transcript in haemocytes of F. indicus increased at 6 h post injection of peptidoglycan and Vibrio harveyi. The Fi-Pxn was mainly expressed in the tissues of haemocytes and the heart. The moulting stage responses showed Fi-Pxn expression in premoult stages D0/1 and D0/2. PMID:25072536

  11. Haemocyanin content of shrimp (Fenneropenaeus chinensis) associated with white spot syndrome virus and Vibrio harveyi infection process.

    Science.gov (United States)

    Chang, Yanhong; Xing, Jing; Tang, Xiaoqian; Sheng, Xiuzhen; Zhan, Wenbin

    2016-01-01

    Haemocyanin (Hc) is frequently reported to vary significantly by physiological status and environmental stress in Crustaceans. In this paper, the shrimp Fenneropenaeus chinensis was infected with different concentrations of white spot syndrome virus (WSSV) and Vibrio harveyi. Then, the variation of Hc and total protein content of the haemolymph (TPCH) were investigated using the established double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) and Coomassie brilliant blue method, respectively. The results showed that the Hc content peaked at 12 h post-infection (PI) in the 10(-2), 10(-4) and 10(-6) viral supernatant (VS) groups, and the maximum was 93.03 ± 2.55 mg ml(-1), 77.57 ± 6.02 mg ml(-1) and 70.25 ± 3.96 mg ml(-1), respectively. TPCH reached the maximum of 108.18 ± 1.36 mg ml(-1) and 103.49 ± 1.33 mg ml(-1) at 12 h PI in the 10(-2) and 10(-4) VS groups, respectively. The maximum was 96.94 ± 1.06 mg ml(-1) at 24 h PI in the 10(-6) VS group. In the V. harveyi infection groups, the Hc content reached a maximum of 87.97 ± 4.39 mg ml(-1) at 36 h PI in the 10(6) CFU ml(-1) group, 73.74 ± 4.38 mg ml(-1) and 72.47 ± 2.09 mg ml(-1) at 12 h PI in the 10(7) and 10(8) CFU ml(-1) groups, respectively. TPCH reached a maximum of 111.16 ± 0.86 mg ml(-1) at 36 h PI in the 10(6) CFU ml(-1) group, 100.41 ± 0.51 mg ml(-1) and 101.94 ± 0.47 mg ml(-1) at 12 h PI in the 10(7) and 10(8) CFU ml(-1) groups, respectively. These data showed that both Hc content and TPCH varied as the same extent after infection. The up-regulation of the Hc content at 6-36 h PI might be a reference threshold for shrimp infection. PMID:26616234

  12. Chromosome Behavior of Heat Shock Induced Triploid in Fenneropenaeus Chinensis

    Institute of Scientific and Technical Information of China (English)

    张晓军; 李富花; 相建海

    2003-01-01

    Triploidy was induced in Chinese shrimp Fenneropenaeus chinensis by 30 + 0.5 ℃ heat shock treatment (initiated at 20 min after fertilization) for 10 min to inhibit the release of PB2 at 18.0℃ . The highest triploid rate obtained was 84.5% in nauplius stage. The effect of heat shock treatment on meiosis and cleavage of eggs was investigated in this work aimed to establish ef ficient procedures for triploid induction and to gain understanding of the mechanism of triploid production. Three pronuclei that could be observed in the treated eggs under fluorescence microscope developed into triploid embryos. Some abnormal chromosome behavior was observed in heat shocked eggs.

  13. Comparative growth and viability of hybrids between two populations of Chinese shrimp (Fennropenaeus chinensis)

    Institute of Scientific and Technical Information of China (English)

    TIAN Yi; KONG Jie; YANG Cuihua

    2006-01-01

    Fenneropenaeus chinensis is confined to the Yellow Sea and Bohai Sea in China and the West Coast of the Korean Peninsula. Intra- and intercross populations were produced between Rushany (YP) and Korean (KN) populations. Seven traits were recorded. The heterosis of hybrids was computed and comparison between treatments was performed by ANOVA. At the fourth month after post-larvae, six indexes of growth trait and viability showed a range of heterosis, ranging from 0.514% to 14.950%. At the fifth month after post-larvae, six indexes of growth trait and viability ranged from -9.000% to 19.090%. The negative heterosis was observed in CL, HST and viability. The heterosis of KN♀×YP♂ tended generally to increase as the age of the Chinese shrimp increased while the heterosis of YP♀×KN♂ tended to decrease. The results indicated that the viability of reciprocal hybrids were not significantly different (P>0.05) from their parents during the experiment. The result of ANOVA indicated that the F1 hybrids were significantly different (P<0.05) in WST and TW at the fourth month. The multiple comparisons of LSD test indicated that the hybrids of YP♀×KN♂ were significantly different (P<0.05) from their parents in TW. The hybrids of YP♀×KN♂ were significantly different (P<0.05) from the other three combinations in WST. At the fifth months, the F1 hybrids had significant difference (P<0.05) in body weight while other traits showed no significant differences (P>0.05) from their parents. The multiple comparisons of LSD test indicated that the hybrids of KN♀×YP♂ were significantly different (P<0.05) from the KN parents in TW. The results indicate that in experimental conditions, the F1 hybrids created from two populations of Chinese shrimp showed a certain level of heterosis for growth performance and viability. The crossing scheme may improve growth performance and viability in Chinese shrimp, but the improvement may be limited because effective

  14. Influence of Agathi grandiflora active principles inhibit viral multiplication and stimulate immune system in Indian white shrimp Fenneropenaeus indicus against white spot syndrome virus infection.

    Science.gov (United States)

    Bindhu, Francis; Velmurugan, Subramanian; Donio, Mariathason Birdilla Selva; Michaelbabu, Mariavincent; Citarasu, Thavasimuthu

    2014-12-01

    Five herbs including Adathoda vasica, Agathi grandiflora, Leucas aspera, Psoralea corylifolia, and Quercus infectoria were selected to screen the antiviral and immunostimulant activity against white spot syndrome virus (WSSV) and Vibrio harveyi respectively using different organic polar and non-polar solvents. Based on the initial screening results, ethyl acetate and methanolic extracts of A. grandiflora had strong antiviral and immunostimulant activities. Those extracts incubated with WSSV injected Fenneropenaeus indicus got only 20% mortality and no PCR positive signals were seen in two step PCR amplification. The methanolic extracts of A. grandiflora were further purified through silica column chromatography and the fractions screened again for antiviral and immunostimulant activity. The secondary screening results revealed that, the fractions of F5 to F7 had effectively controlled the WSSV multiplication and V. harveyi growth. The pooled fractions (F5 to F7) was structurally characterized by gas chromatograph-mass spectrometry (GC-MS) analysis and few compounds were identified including 3,7.11,15-Tetramethyl-2-Hexane-1-ol, pytol and 1,2-Benzenedicarboxylic acid, diisooctyl ester. The pooled fractions were mixed with the basal feed ingredients at the concentration of 100 (D-1), 200 (D-2), 300 (D-3) and 400 (D-4) mg kg(-1) and the diets fed to the F. indicus (9.0 ± 0.5 g) for 30 days. After the completion of feeding trail, they were challenged with virulent WSSV and studied the cumulative mortality, molecular diagnosis by quantitative real time PCR (qRT-PCR), biochemical, haematological and immunological parameters. The control diet fed F. indicus succumbed to death 100% within 3 days whereas the D-3 and D-4 helped to reduced the cumulative mortality of 60-80% respectively. The qRT-PCR revealed that, the WSSV copy number was gradually decreased when increasing concentration of A. grandiflora extract active fraction in the diets. The diets D-3 and D-4 helped to

  15. Cloning, sequencing and expression analysis of cDNA encoding a constitutive heat shock protein 70 (HSC70) in Fenneropenaeus chinensis

    Institute of Scientific and Technical Information of China (English)

    JIAO Chuanzhen; WANG Zaizhao; LI Fuhua; ZHANG Chengsong; XIANG Jianhai

    2004-01-01

    The cDNA encoding hsc70 of Chinese shrimp Fenneropenaeus chinensis was cloned from hepatopancreas by RT-PCR based on its EST sequence. The full length cDNA of 2090 bp contained an open reading frame of 1956 nucleotides and partial 5′- and 3′-untranslated region(5′- and 3′-UTR). PCR amplification and sequencing analysis showed the existence of introns in the region of 1-547 bp, but they did not exist in the region of 548-2090 bp of hsc70 cDNA. When the deduced 652 amino acid sequence of HSC70 was compared with the members of HSP70 family from other organisms, the results showed 85.9% similarity with HSC71 from Oncorhynchus mykiss and HSC70 from Homo sapiens. It also exhibited 85.8% similarity with HSP70 from Mus musculu and 85.4% with HSC70 from Manduca sexta. Expression analysis showed that hsc70 mRNA was espressed constitutively in hepatopancreas, muscle, eyestalks, haemocytes, heart, ovary, intestine and gills in Fenneropenaeus chinensis. No difference could be detected on hsc70 mRNA level in muscle between heat-shocked and control animals.

  16. Foreign gene transfer into Chinese shrimps (Penaeus chinensis) with gene gun

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Plasmids pG DNA-RZ1 with a GFP (green fluorescent protein) reporter gene and a ribozyme gene incising penaeid white spot baculovirus (WSBV) were first introduced into the fertilized eggs of Chinese shrimps by gene gun. The treated and control samples of different development stages were observed with a fluorescent microscope. The transient expression of GFP gene was high in nauplius and zoea larvae. Results from RT-PCR and PCR for adults showed that the foreign genes had been transferred into the shrimps and had expressed the corresponding proteins. This work has established a transgenic method for penaeid shrimps, which will set base for the application of genetic engineering breeding into industry.

  17. Life cycle assessment of Chinese shrimp farming systems targeted for export and domestic sales.

    Science.gov (United States)

    Cao, Ling; Diana, James S; Keoleian, Gregory A; Lai, Qiuming

    2011-08-01

    We conducted surveys of six hatcheries and 18 farms for data inputs to complete a cradle-to-farm-gate life cycle assessment (LCA) to evaluate the environmental performance for intensive (for export markets in Chicago) and semi-intensive (for domestic markets in Shanghai) shrimp farming systems in Hainan Province, China. The relative contribution to overall environmental performance of processing and distribution to final markets were also evaluated from a cradle-to-destination-port perspective. Environmental impact categories included global warming, acidification, eutrophication, cumulative energy use, and biotic resource use. Our results indicated that intensive farming had significantly higher environmental impacts per unit production than semi-intensive farming in all impact categories. The grow-out stage contributed between 96.4% and 99.6% of the cradle-to-farm-gate impacts. These impacts were mainly caused by feed production, electricity use, and farm-level effluents. By averaging over intensive (15%) and semi-intensive (85%) farming systems, 1 metric ton (t) live-weight of shrimp production in China required 38.3 ± 4.3 GJ of energy, as well as 40.4 ± 1.7 t of net primary productivity, and generated 23.1 ± 2.6 kg of SO(2) equiv, 36.9 ± 4.3 kg of PO(4) equiv, and 3.1 ± 0.4 t of CO(2) equiv. Processing made a higher contribution to cradle-to-destination-port impacts than distribution of processed shrimp from farm gate to final markets in both supply chains. In 2008, the estimated total electricity consumption, energy consumption, and greenhouse gas emissions from Chinese white-leg shrimp production would be 1.1 billion kW·h, 49 million GJ, and 4 million metric tons, respectively. Improvements suggested for Chinese shrimp aquaculture include changes in feed composition, farm management, electricity-generating sources, and effluent treatment before discharge. Our results can be used to optimize market-oriented shrimp supply chains and promote more

  18. Isolation and expression analysis of an MAPKK gene from Fenneropenaeus chinensis in response to white spot syndrome virus infection.

    Science.gov (United States)

    Li, Xupeng; Kong, Jie; Meng, Xianhong; Luo, Kun; Luan, Sheng; Cao, Baoxiang; Liu, Ning

    2016-08-01

    Mitogen-activated kinase kinase (MAPKK) is an important gene involved in the host-virus interaction process. To obtain a better understanding of MAPKK in the interaction process between the Chinese shrimp Fenneropenaeus chinensis and white spot syndrome virus (WSSV), we cloned the sequence of an MAPKK cDNA from F. chinensis (FcMAPKK) and investigated the effect of FcMAPKK on WSSV infection. The results showed that the FcMAPKK gene contained a 1227 bp open reading frame (ORF), which encoded a highly conserved protein with a serine/threonine protein kinase catalytic (S_TKc) domain. The deduced amino acid sequence of FcMAPKK shared identities between 11.9 and 92.6% with MAPKKs from vertebrate, invertebrate, plant and fungus species. The FcMAPKK was expressed in all the examined tissues in the normal F. chinensis. FcMAPKK expression level was highest in the hepatopancreas where it was approximately 2.6-fold the expression level in the gill, and lowest in the muscle where it was approximately 0.3-fold the expression level in the hepatopancreas. The FcMAPKK expression levels in the muscle, gill, and hepatopancreas were all changed post WSSV challenge. The FcMAPKK expression was significantly (P process, and hijacking of FcMAPKK might be required for WSSV replication in F. chinensis. PMID:27164214

  19. 中国对虾体内1株益生菌的筛选与初步鉴定%Screening and Identification of a Potential Probiotic in Shrimp, Fenneropenaeus chinensis

    Institute of Scientific and Technical Information of China (English)

    张玲; 谭北平; 麦康森; 艾庆辉; 张文兵; 徐玮

    2008-01-01

    为给对虾专用益生菌制剂的研制与应用提供理论依据和基础数据,研究了从白斑病毒(WSSV) 耐过中国对虾(Fenneropenaeus chinensis)肠道中分离的1株海洋细菌B12的益生特性和安全性,并结合形态观察、生理生化特征和16S rDNA序列分析对菌株B12进行了分类鉴定.结果表明:菌株B12能抑制对虾致病性哈维氏弧菌 (Vibrio harveyi)和副溶血弧菌(V. parahaemolyticus)的生长,不分泌溶血素.对β-内酰胺类、头孢类、氨基糖苷类、喹诺酮类抗生素均敏感;对大环内酯类、四环素类抗生素均耐药;对糖肽类抗生素中度敏感.毒性试验表明菌株B12对中国对虾幼体没有明显毒副作用.该菌为革兰氏阴性可动杆菌,鞭毛极生,菌体大小为(0.5~0.6)×(1.1~1.2) μm,接触酶、氧化酶阳性,葡萄糖发酵产酸,能还原硝酸盐,产淀粉酶,不产明胶酶,不能利用丙二酸.16S rDNA的部分序列分析显示菌株B12与嗜盐单胞菌(Halomonas sp.)SB J85具有98.15%的相似性.形态观察、生理生化特征和16S rDNA序列分析证实B12为嗜盐单胞菌属(Halomonas sp.).

  20. cDNA cloning, characterization and expression analysis of a novel antimicrobial peptide gene penaeidin-3 (Fi-Pen3) from the haemocytes of Indian white shrimp Fenneropenaeus indicus.

    Science.gov (United States)

    Shanthi, S; Vaseeharan, B

    2012-03-20

    A new member of antimicrobial peptide genes of the penaeidin family, penaeidin 3, was cloned from the haemocytes of Indian white shrimp Fenneropeneaus indicus (F. indicus), by reverse transcription PCR (RT-PCR) and rapid amplification of cDNA end (RACE-PCR) methods. The complete nucleotide sequence of cDNA clone of Indian white shrimp F. indicus Penaeidin 3 (Fi-Pen3) was 243bp long and has an open reading frame which encodes 80 amino acid peptide. The homology analysis of Fi-Pen3 sequence with other Penaeidins 3 shows higher similarity with Penaeus monodon (92%). The theoretical 3D structure generated through ab initio modelling indicated the presence of two-disulphide bridges in the alpha-helix. The signal peptide sequence of Fi-Pen3 is almost entirely homologous to that of other Penaeidin 3 of crustaceans, while differing relatively in the N-terminal domain of the mature peptide. The mature peptide has a predicted molecular weight of 84.9kDa, and a theoretical pI of 9.38. Phylogenetic analysis of Fi-Pen3 shows high resemblance with other Pen-3 from P. monodon, Litopenaeus stylirostris, Litopenaeus vannamei and Litopenaeus setiferus. Fi-Pen3 found to be expressed in haemocytes, heart, hepatopancreas, muscles, gills, intestine, and eyestalk with higher expression in haemocytes. Microbial challenge resulted in mRNA up-regulation, up to 6h post injection of Vibrio parahemolyticus. The Fi-Pen3 mRNA expression of F. indicus in the premolt stage (D(01) and D(02)) was significantly up-regulated than the postmolt (A and B) and intermolt stages (C). The findings of the present paper underline the involvement of Fi-Pen3 in innate immune system of F. indicus. PMID:21885268

  1. Differentially proteomic analysis of the Chinese shrimp at WSSV latent and acute infection stages by iTRAQ approach.

    Science.gov (United States)

    Li, Shihao; Li, Fuhua; Sun, Zheng; Zhang, Xiaojun; Xiang, Jianhai

    2016-07-01

    As the direct executors of biological function, the expression level of proteins will reveal the molecular mechanisms regulating WSSV acute infection more directly. In the present study, the iTRAQ approach was applied to identifying differentially expressed proteins in Chinese shrimp during WSSV latent infection and acute infection. A total of 4051 unique peptides corresponding to 1286 proteins were identified. 118 unique proteins showed differential up-regulation and 122 proteins were down-regulated in shrimp during WSSV acute infection compared with those in WSSV latent infection stage. A number of proteins related to actin-myosin cytoskeleton process, including myosin, actin, tubulin, clathrin, and tropomyosin were found up-regulated in shrimp at WSSV AI stage, indicating that the phagocytosis process was involved in WSSV AI stage. The apoptosis process in shrimp during WSSV AI seemed to be inhibited because some proteins suppressive on apoptosis were up-regulated, such as ALG-2 interacting protein x, Hsp90, 14-3-3-like protein, peroxiredoxin 5, peroxiredoxin 6 and adenine nucleotide translocase 2. Association analysis between the proteomic data and the previous transcriptome data was performed. Quantitative real-time PCR and western blot were carried out to verify the reliability of the proteomics data. The present study provided a comprehensive view of molecular mechanisms regulating WSSV acute infection at the protein level. PMID:27192146

  2. Lack of gender effect on the pharmacokinetics of oxytetracycline in Fenneropenaeus chinensis after intramuscular administration

    Science.gov (United States)

    Sun, Ming; Li, Jian; Chang, Zhiqiang; Guo, Wenting; Zhao, Fazhen; Li, Jitao

    2015-04-01

    Fenneropenaeus chinensis, an economically important shrimp species, currently suffers from epizootic diseases due to high density stocking and bacterial infections. Oxytetracycline (OTC) has been widely used to treat various systemic bacterial infections in shrimp farming. In the present study, the effect of gender on pharmacokinetics of OTC in F. chinensis was investigated. The OTC concentrations in hemolymph of shrimp after single intramuscular administration (75 mg OTC per kg body weight) were analyzed by high performance liquid chromatography and best described with a two-compartment open model which is characterized by a short elimination half-life, low clearance, and a relatively large apparent volume of distribution. The pharmacokinetic equations were C t = 58.54e-0.38 t + 11.67e-0.04 t for females; and C t = 27.94e-0.28 t + 14.87e-0.04 t for males. The distribution and elimination half-lives of OTC were 1.82 and 19.58 h, respectively, in females and 2.50 and 16.11 h, respectively, in males at 22°C. The areas under the drug concentration curve were 480 mg L-1 h-1 in females and 430 mg L-1 h-1 in males. The total body clearance of the drug was 157.11 mL kg-1 h-1 in females and 172.47 mL kg-1 h-1 in males. The apparent volume distribution was 4.44 in females and 4.01 L kg-1 in males. There was no significant difference in pharmacokinetic parameters between female and male shrimps, indicating that there is no need to consider the gender effect in clinical use of OTC in F. chinensis farming.

  3. Effects of salinity fluctuation in different ranges on the intermolt period and growth of juvenile Fenneropenaeus chinensis

    Institute of Scientific and Technical Information of China (English)

    MU Yingchun; WANG Fang; DONG Shuanglin; DONG Shaoshuai; ZHU Changbo

    2005-01-01

    Experiments were conducted to examine the effects of salinity fluctuation on the intermolt period and growth of Fenneropenaeus chinensis with an initial body weight of (0.553 2±0.000 1)g (mean plus or minus SE). The salinity (S0) of the control treatment is 30throughout the experiment, while the treatments S2, S4, S7 and S10 are subjected to different salinity fluctuations, and the ranges are 2, 4,7 and 10, respectively. After a 30-day feeding trial, the longest and the shortest intermolt periods of the shrimp occur under Treatments S10 and S2, respectively (difference 37.14%). The special growth rates (SGR) of the shrimp under the five treatments are ranked as S7>S4> S2> S10>S0, and the SGR of the shrimp under Treatment S0 is lower than that under Treatments S4 and S7 by 22.36% and 28.11%,respectively (P<0.05). The minimal feed intake (FI) of the experimental animals occurs in Treatment S0 (84.04% and 88.54% of that under Treatments S4 and S7, respectively) (P<0.05). There are no significant differences in the food conversion efficiency (FCE) of the shrimp under the five treatments (P>0.05).

  4. The complete mitochondrial genomes of the yellowleg shrimp Farfantepenaeus californiensis and the blue shrimp Litopenaeus stylirostris (Crustacea: Decapoda).

    Science.gov (United States)

    Peregrino-Uriarte, Alma B; Varela-Romero, Alejandro; Muhlia-Almazán, Adriana; Anduro-Corona, Iván; Vega-Heredia, Sarahí; Gutiérrez-Millán, Luis E; De la Rosa-Vélez, Jorge; Yepiz-Plascencia, Gloria

    2009-03-01

    Mitochondria play key roles in many cellular processes. Description of penaeid shrimp genes, including mitochondrial genomes are fairly recent and some are focusing on commercially important shrimp as the Pacific shrimp Litopenaeus vannamei that is being used for aquaculture not only in America, but also in Asia. Much less is known about other Pacific shrimp such as the yellowleg shrimp Farfantepenaeus californiensis and the blue shrimp Litopenaeus stylirostris. We report the complete mitogenomes from these last two Pacific shrimp species. Long DNA fragments were obtained by PCR and then used to get internal fragments for sequencing. The complete F. californiensis and L. stylirostris mtDNAs are 15,975 and 15,988 bp long, containing the 37 common sequences and a control region of 990 and 999 bp, respectively. The gene order is identical to that of the tiger shrimp Penaeus monodon. Secondary structures for the 22 tRNAs are proposed and phylogenetic relationships for selected complete crustacean mitogenomes are included. Phylogenomic relationships among five shrimp show strong statistical support for the monophyly of the genus across the analysis. Litopenaeus species define a clade, with close relationship to Farfantepenaeus, and both clade with the sister group of Penaeus and Fenneropenaeus. PMID:20403743

  5. Primary carbon sources for juvenile penaeid shrimps in a mangrove-fringed Bay of Inhaca Island, Mozambique: a dual carbon and nitrogen isotope analysis.

    OpenAIRE

    Macia, A.

    2004-01-01

    A study to estimate the relative importance of mangrove primary carbon and nitrogen sources to five commercial penaeid shrimps species was done at Saco da Inhaca, a non-estuarine mangrove-fringed bay on Inhaca Island, southern Mozambique. Carbon and nitrogen stable isotope ratios were determined in a variety of primary producers (mangroves, epiphytes, phytoplankton and seagrasses), sediments and in five penaeid shrimp species (Penaeus (Fenneropenaeus) indicus, P. japonicus, P. semisulcatus, M...

  6. SUMO and SUMO-Conjugating Enzyme E2 UBC9 Are Involved in White Spot Syndrome Virus Infection in Fenneropenaeus chinensis.

    Directory of Open Access Journals (Sweden)

    Xiaoqian Tang

    Full Text Available In previous work, small ubiquitin-like modifier (SUMO in hemocytes of Chinese shrimp Fenneropenaeus chinensis was found to be up-regulated post-white spot syndrome virus (WSSV infection using proteomic approach. However, the role of SUMO in viral infection is still unclear. In the present work, full length cDNAs of SUMO (FcSUMO and SUMO-conjugating enzyme E2 UBC9 (FcUBC9 were cloned from F. chinensis using rapid amplification of cDNA ends approach. The open reading frame (ORF of FcSUMO encoded a 93 amino acids peptide with the predicted molecular weight (M.W of 10.55 kDa, and the UBC9 ORF encoded a 160 amino acids peptide with the predicted M.W of 18.35 kDa. By quantitative real-time RT-PCR, higher mRNA transcription levels of FcSUMO and FcUBC9 were detected in hemocytes and ovary of F. chinensis, and the two genes were significantly up-regulated post WSSV infection. Subsequently, the recombinant proteins of FcSUMO and FcUBC9 were expressed in Escherichia coli BL21 (DE3, and employed as immunogens for the production of polyclonal antibody (PAb. Indirect immunofluorescence assay revealed that the FcSUMO and UBC9 proteins were mainly located in the hemocytes nuclei. By western blotting, a 13.5 kDa protein and a 18.7 kDa protein in hemocytes were recognized by the PAb against SUMO or UBC9 respectively. Furthermore, gene silencing of FcSUMO and FcUBC9 were performed using RNA interference, and the results showed that the number of WSSV copies and the viral gene expressions were inhibited by knockdown of either SUMO or UBC9, and the mortalities of shrimp were also reduced. These results indicated that FcSUMO and FcUBC9 played important roles in WSSV infection.

  7. 中国对虾素在大肠杆菌(Escherichia coli)中的重组表达%RECOMBINANT EXPRESSION OF CH-PENAEIDIN FROM CHINESE SHRIMP FENNEROPENAEUS CHINENSIS IN ESCHERICHIA COLI

    Institute of Scientific and Technical Information of China (English)

    薛剑峰; 康翠洁; 王金星; 赵小凡; 相建海

    2005-01-01

    中国对虾素是从中国对虾血细胞中克隆得到的一种抗菌肽.为了进一步研究中国对虾素(CHP)的功能并为制备特异性抗体作准备,采用大肠杆菌表达外源蛋白的方法,进行了对虾素原核表达的研究.根据从中国对虾血细胞中克隆得到的对虾素基因设计特异性引物扩增中国对虾素成熟肽基因(Chp),插入原核表达载体pGEX-4T-1中,在E.coli BL21表达融合蛋白GST-CHP.结果表明,不同表达菌株的融合蛋白表达量为30%-34%,同一菌株在诱导5h后能达到最高表达量.利用GST亲和层析纯化融合蛋白,将融合蛋白用凝血酶裂解以得到CHP,其分子量约为5.6kDa,N-端测序结果与期望的成熟对虾肽序列一致.用液体生长抑制方法检测活性,表明重组GST-CHP蛋白及CHP均表现出对大肠杆菌的抑菌活性.

  8. Effects of Dietary Fly Maggot Musca domestica on Growth and Body Compositions in Chinese Shrimp Fenneropenaeus chinensis Juveniles%投喂蝇蛆对中国明对虾生长及生化组成的影响

    Institute of Scientific and Technical Information of China (English)

    郑伟; 董志国; 王兴强; 曹梅; 阎斌伦; 李士虎

    2010-01-01

    在试验生态水槽系统内,研究蝇蛆在日粮中不同配比对中国明对虾幼虾生长及生化组成的影响.以蝇蛆在日粮中的所占比例设计了5个梯度组合,经过35 d投喂,对虾体长、体质量、特定生长率和成活率升高,对虾肌肉水解氨基酸的总量及其中的必需氨基酸、呈味氨基酸含量增加;在配合饲料中混合投喂3/8以上的蝇蛆可显著提高中国明对虾幼虾的生长速率(P<0.05).对虾肌肉中∑n-3 HUFA、∑n-6 HUFA的相对含量随着蝇蛆投喂量的增加而增加,蝇蛆投喂组的必需脂肪酸相对含量均显著低于对照组,多不饱和脂肪酸显著高于对照组.C22∶6n-3/C18∶2n-6比值显著高于对照组,且随着蝇蛆投喂量的增加比值增加.

  9. Analysis of the genetic diversity and differentiation of Fenneropenaeus penicillatus populations using AFLP technology

    Institute of Scientific and Technical Information of China (English)

    ZHANG Guiling; CAO Yuanyu; LI Zhongbao; CHEN Jin; ZHAO Binli; LEI Guanggao; WANG Zhanlin

    2012-01-01

    Fenneropenaeus penicillatus (redtail shrimp) is an important marine commercial animal in China.Recently,its resources have been depleted rapidly as a result of,for example,over-exploitation and environmental degradation of spawning grounds.Therefore,we analyzed the genetic diversity and differentiation of nine wild populations of F.penicillatus of China (Ningde,Lianjiang,Putian,Xiamen,Quanzhou,Zhangpu,Dongshan,Nanao,and Shenzhen populations) by amplified fragment length polymorphism (AFLP) technology,to provide genetic information necessary for resource protection,rejuvenation,artificial breeding,and sustainable use of the resource.Eight AFLP primer pairs were used for amplification,and 508 bands were detected among the populations.The results show that the percentage of polymorphic loci (P) ranged from 41.34% to 63.58%; the Nei's gene diversity (H) of the populations was 0.119 4-0.230 5; and Sharnon's Information Index (I) was 0.184 1-0.342 5.These genetic data indicate that the genetic diversity of F.penicillatus was high.The genetic differentiation coefficient (Gsr=0.216 2) and gene flow (Nm=1.812 4) show that there was a high level of genetic differentiation and a moderate level of gene flow among populations.More studies on the genetic differentiation mechanism of F.penicillatus along the south-eastern coast of China need to be conducted to find more effective scientific protection strategies for the conservation ofF.penicillatus genetic resources.

  10. Establishment of two-dimensional electrophoresis (2-DE) technique in muscle proteome of Fenneropenaeus chinensis%中国明对虾肌肉组织蛋白质双向电泳技术体系的建立

    Institute of Scientific and Technical Information of China (English)

    刘志远; 励建荣; 李学鹏; 李婷婷; 王彦波; 黄和; 陈华健; 陆维克

    2013-01-01

    In order to use two-dimensional electrophoresis(2-DE)analysis in the muscle proteins of Chinese shrimp(Fenneropenaeus chinensis) ,its muscle proteins were separated using immobilized pH gradient 2-DE after dissolving. By optimizing different extraction methods,pH of IPG gel strips and its length,isoelectric focusing programs, and loading amount, etc, the proteins were successfully extracted from Chinese shrimp muscle and were separated by 2-DE. After silver staining or Coomassie brilliant blue staining,PDQuest image analysis software was applied to analyze the 2-DE images. The results showed that most of shrimp muscle protein isoelectric points were between 4 and 7. The 2-DE related techniques was constructed and optimized in muscle proteome of Chinese shrimp by comparative tests on different extraction methods, IPG gel strips, isoelectric focusing programs,salt bridge,and sample volume,etc. The results showed that the resolution and reproducibility of 2-DE profiles were significantly improved by adding thiourea, CHAPS and DTT in lysis buffer,active rehydrating of 17 cm(pH 4 -7)IPG gel strips,loading the sample 120 |xg,prolonging the time of desalting, increasing the voltage and power of isoelectric focusing, employing the salt bridge, preparing 12. 5% SDS-PAGE gel,and dying the gels by the silver staining. It shows that the repeatability and stability are good enough.%为了探索并建立中国明对虾的肌肉蛋白双向电泳体系,实验将中国明对虾的肌肉组织溶解处理后,通过固相pH梯度胶条等电聚焦、SDS-PAGE垂直电泳对蛋白质进行分离,对不同的裂解液配方、IPG胶条的pH范围及其长度、等电聚焦程序、上样量等进行了优化,并分别利用银染和考染方法进行染色,应用PDQuest软件对图谱进行了初步分析.结果显示,中国明对虾肌肉蛋白的等电点主要位于4~7之间,裂解液中添加硫脲、CHAPS、DTT等,可以增加对虾肌肉蛋白的提取率;采用17 cm,pH 4~7

  11. Environmental and human health risks of antimicrobials used in Fenneropenaeus chinensis aquaculture production in China.

    Science.gov (United States)

    Sun, Ming; Chang, Zhiqiang; Van den Brink, Paul J; Li, Jian; Zhao, Fazhen; Rico, Andreu

    2016-08-01

    This study aimed to quantify the environmental fate of antimicrobials applied in Fenneropenaeus chinensis aquaculture production in China and to assess their potential risks for surrounding aquatic ecosystems, for the promotion of antimicrobial resistance in target and non-target bacteria and for consumers eating shrimp products that contain antimicrobial residues. For this, we first used the results of an environmental monitoring study performed with the antimicrobial sulfamethazine to parameterize and calibrate the ERA-AQUA model, a mass balance model suited to perform risk assessments of veterinary medicines applied in aquaculture ponds. Next, a scenario representing F. chinensis production in China was built and used to perform risk assessments for 21 antimicrobials which are regulated for aquaculture in China. Results of the model calibration showed a good correspondence between the predicted and the measured sulfamethazine concentrations, with differences within an order of magnitude. Results of the ecological risk assessment showed that four antimicrobials (levofloxacin, sarafloxacin, ampicillin, sulfadiazine) are expected to have adverse effects on primary producers, while no short-term risks were predicted for invertebrates and fish exposed to farm wastewater effluents containing antimicrobial residues. Half of the evaluated antimicrobials showed potential to contribute to antimicrobial resistance in bacteria exposed to pond water and farm effluents. A withdrawal period of three weeks is recommended for antimicrobials applied via oral administration to F. chinensis in order to comply with the current national and international toxicological food safety standards. The results of this study indicate the need to improve the current regulatory framework for the registration of aquaculture antimicrobials in China and suggest compounds that should be targeted in future aquaculture risk assessments and environmental monitoring studies. PMID:27137193

  12. Monoclonal antibody based immunodot for specific detection of proteins of the shrimp Penaeus species.

    Science.gov (United States)

    Abhiman, P B; Shankar, K M; Patil, Rajreddy; Suresh Babu, P P; Sahoo, A K; Shamasundar, B A

    2014-05-01

    Frozen shrimp continued to be the single largest item of export from India in terms of value accounting for about 44% of the total marine export earnings. Headless, peeled frozen shrimp is a common and dominant item in the market and there is need for differentiating peeled Penaeus sp from Metapenaeus, Parapenopsis and Macrobrachium sp as consumer preference and price vary. Furthermore, there is need to find out original species used in value addition of shrimp products. Hence, it is essential for development of simple and consumer friendly technique for the identification of shrimp and their products in the market. Two monoclonal antibodies (MAbs) C-15 (IgG3) and C-52 (IgG2a) reacting with 65 and 47 kD proteins of Penaeus monodon respectively in the Western blot were selected. In epitope analysis by immunodot, the two MAbs reacted and recognized specific proteins of P. monodon, Fenneropenaeus indicus and Littopenaeus vannamei and not that of Metapenaeus, Parapenopsis, Macrobrachium rosenbergii, crabs and fishes. The immunodot required 120 min for completion. The sensitivity of the immunodot to detect proteins of P. monodon was 0.225 mg with MAb C-15 and 0.028 mg with MAb C-52. The MAb based immunodot developed, could be used for identifying and differentiating meat of P. monodon, F. indicus, and L. vannamei from that of Metapenaeus, Parapenopsis, M. rosenbergii, crabs and fishes. PMID:24803705

  13. Shrimp Survey Data

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Northern Shrimp Survey was initiated in 1983 by the Atlantic States Marine Fisheries Commission (ASMFC) and monitors the relative abundance (number of shrimp),...

  14. Shrimp Farming in the Classroom.

    Science.gov (United States)

    Ruggiero, Lovelle

    2000-01-01

    Describes a project on culturing shrimp. Presents observational labs and the experimentation procedure. Provides general information about shrimp, their life span, optimum temperatures, and other important information. (YDS)

  15. The effects of L-ascorbyl-2-polyphosphate on the growth and immunity of chinese shrimp (Fenneropenaeus chinensis) juvenile%Vc-2-多聚磷酸酯对中国对虾稚虾生长、免疫及体内Vc含量的影响

    Institute of Scientific and Technical Information of China (English)

    秦志华; 李健; 王群; 刘淇

    2007-01-01

    用添加不同浓度LAPP的饲料投喂中国对虾30 d,每隔5 d取对虾血淋巴,测定血清中超氧化物歧化酶活力、溶菌活力、酚氧化酶活力;试验结束时测定盐度突变对中国对虾成活率的影响及其体内维生素C积累量的变化情况.结果显示,高浓度LAPP组对虾较对照组及低浓度组3种酶活力都达到了显著差异(P<0.05),但是酶活力并不是持续升高,一段时间后又有所回落;盐度突变试验结果表明,添加0.3%组显著提高了对虾的成活率;HPLC法测定对虾体内维生素C积累量的变化情况显示,在一定范围内对虾体内的维生素C积累量随着饲料中维生素C添加水平的提高而提高.在本试验条件下,建议中国对虾稚虾饲料LAPP的适宜添加量为3 000 mg/kg.

  16. Antimicrobial and Antifungal Effects of Acid and Water-Soluble Chitosan Extracted from Indian Shrimp (Fenneropenaeus indicus Shell

    Directory of Open Access Journals (Sweden)

    Ali Taheri

    2013-06-01

    Full Text Available Background & Objective : Currently, efforts are underway to seek new and effective antimicrobial agents, and marine resources are potent candidates for this aim. The following study was conducted to investigate the efficacy of water-soluble and acid-soluble chitosan against some pathogenic organisms.   Materials & Method s: Inhibition zone of different concentrations (5, 7.5, and 10 mg/ml of acid- soluble and water-soluble chitosan were examined for in vitro antibacterial activity against 4 kinds of hospital bacteria and penicillium sp. Results were compared with 4 standard antibiotics: streptomycin, gentamicin, tetracycline, and erythromycin. Furthermore, minimum inhibitory concentration and minimum lethal concentration were determined.   Results: Inhibition activity of acid-soluble chitosan (10% showed the best result (p value < 0.05, whereas water-soluble chitosan exhibited the least antibacterial effects (p value < 0.05. Chitosan demonstrated maximum effect on V. cholera cerotype ogava , and the least effect was seen on E. coli (p value < 0.05. Acid-soluble chitosan had a more potent effect than the standard antibiotics. Also, acid-soluble chitosan (10% and water-soluble chitosan showed maximum inhibitory effects on penicillium sp.   Conclusion: Chitosan showed maximum antibacterial effect against S. aureus, V. cholerae cerotype ogava, and water-soluble chitosan demonstrated good antifungal effects, revealing a statistically significant difference with common antibacterial and antifungal medicines.

  17. Effects on survival time of Fenneropenaeus chinensis challenged by different doses of WSSV and diets%WSSV人工感染量和饵料对中国明对虾存活时间的影响

    Institute of Scientific and Technical Information of China (English)

    李旭鹏; 孟宪红; 孔杰; 曹宝祥; 刘宁; 罗坤; 李伟亚

    2013-01-01

    为揭示不同白斑综合征病毒(white spot syndrome virus,WSSV)病毒量对于中国明对虾存活时间和存活率的影响,实验设计了逐尾、定量人工感染实验,在确保每尾中国明对虾进食特定量WSSV毒饵后进行观察、分析.结果显示,分别喂食含5.2×108 copies、1.0×109copies、2.1×109 copies WSSV的毒饵,对虾平均存活时间分别是389.3、323.3和187.3 h,差异极显著(P<0.01);对虾最终累计死亡率都为100%.研究表明,致死量范围内,WSSV的感染量越低,对虾的平均存活时间越长.为了揭示饵料对中国明对虾抗病性能的影响,对已感染WSSV的中国明对虾投喂不同饵料.结果显示,分别喂食活卤虫、鲜蛤肉和配合饲料,对虾平均存活时间分别是281.7、173.9和164.9 h;喂食活卤虫的实验组平均存活时间显著高于喂食配合饲料和鲜蛤肉的实验组(P<0.01);喂食配合饲料和鲜蛤肉的对虾平均存活时间无显著差异(P>0.05);3组累计死亡率都为100%,结果表明,与配合饲料和鲜蛤肉相比,喂食活卤虫更能增强对虾抗WSSV的能力.%White spot syndrome virus(WSSV) is one kind of destructive virus affecting shrimp industry in the world. Output of Fenneropenaeus chinensis which once was a staple species has been greatly affected by outbreak of WSSV since 1993 in China. To produce WSSV-resistance shrimp strains is an important approach to revive the shrimp industry. To select survived F. chinensis as broodstock via WSSV challenge test can increase the efficiency of breeding. However, mortality of shrimps could reach nearly 100% once they were infected with WSSV, which increases the difficulty for shrimp breeding. In this study for the purpose to reveal effect of different WSSV amount on F. chinensis,juvenile F. chinensis were infected with a certain amount of WSSV individually and the survival time and survival rate were observed. The results showed that survival time of shrimps infected

  18. Predicted sub-populations in a marine shrimp proteome as revealed by combined EST and cDNA data from multiple Penaeus species

    Directory of Open Access Journals (Sweden)

    Kotewong Rattanawadee

    2010-11-01

    Full Text Available Abstract Background Many species of marine shrimp in the Family Penaeidae, viz. Penaeus (Litopenaeus vannamei, Penaeus monodon, Penaeus (Fenneropenaeus chinensis, and Penaeus (Marsupenaeus japonicus, are animals of economic importance in the aquaculture industry. Yet information about their DNA and protein sequences is lacking. In order to predict their collective proteome, we combined over 270,000 available EST and cDNA sequences from the 4 shrimp species with all protein sequences of Drosophila melanogaster and Caenorhabditis elegans. EST data from 4 other crustaceans, the crab Carcinus maenas, the lobster Homarus americanus (Decapoda, the water flea Daphnia pulex, and the brine shrimp Artemia franciscana were also used. Findings Similarity searches from EST collections of the 4 shrimp species matched 64% of the protein sequences of the fruit fly, but only 45% of nematode proteins, indicating that the shrimp proteome content is more similar to that of an insect than a nematode. Combined results with 4 additional non-shrimp crustaceans increased matching to 78% of fruit fly and 56% of nematode proteins, suggesting that present shrimp EST collections still lack sequences for many conserved crustacean proteins. Analysis of matching data revealed the presence of 4 EST groups from shrimp, namely sequences for proteins that are both fruit fly-like and nematode-like, fruit fly-like only, nematode-like only, and non-matching. Gene ontology profiles of proteins for the 3 matching EST groups were analyzed. For non-matching ESTs, a small fraction matched protein sequences from other species in the UniProt database, including other crustacean-specific proteins. Conclusions Shrimp ESTs indicated that the shrimp proteome is comprised of sub-populations of proteins similar to those common to both insect and nematode models, those present specifically in either model, or neither. Combining small EST collections from related species to compensate for their

  19. Shrimp arginine kinase being a binding protein of WSSV envelope protein VP31

    Science.gov (United States)

    Ma, Cuiyan; Gao, Qiang; Liang, Yan; Li, Chen; Liu, Chao; Huang, Jie

    2016-03-01

    Viral entry into the host is the earliest stage of infection in the viral life cycle in which attachment proteins play a key role. VP31 (WSV340/WSSV396), an envelope protein of white spot syndrome virus (WSSV), contains an Arg-Gly-Asp (RGD) peptide domain known as a cellular attachment site. At present, the process of VP31 interacting with shrimp host cells has not been explored. Therefore, the VP31 gene was cloned into pET30a (+), expressed in Escherichia coli strain BL21 and purified with immobilized metal ion affinity chromatography. Four gill cellular proteins of shrimp (Fenneropenaeus chinensis) were pulled down by an affinity column coupled with recombinant VP31 (rVP31), and the amino acid sequences were identified with MALDI-TOF/TOF mass spectrometry. Hemocyanin, beta-actin, arginine kinase (AK), and an unknown protein were suggested as the putative VP31 receptor proteins. SDS-PAGE showed that AK is the predominant binding protein of VP31. An i n vitro binding activity experiment indicated that recombinant AK's (rAK) binding activity with rVP31 is comparable to that with the same amount of WSSV. These results suggested that AK, as a member of the phosphagen kinase family, plays a role in WSSV infection. This is the first evidence showing that AK is a binding protein of VP31. Further studies on this topic will elucidate WSSV infection mechanism in the future.

  20. South Atlantic Shrimp System

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The SEFSC, in cooperation with the South Atlantic states, collects South Atlantic shrimp data from dealers and fishermen. These data are collected to provide catch,...

  1. Gulf Shrimp System

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Gulf of Mexico Shrimp Landings - This data set contains catch (landed catch) and effort for fishing trips made by the larger vessels that fish near and offshore for...

  2. Burrowing behavior of penaeid shrimps

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Brown shrimp, Farfantepenaeus aztecus, and white shrimp, Litopenaeus setiferus, were held were held under natural light conditions before experiments. Experiments...

  3. Investigating Brine Shrimp.

    Science.gov (United States)

    Duran, Lena Ballone

    2003-01-01

    Presents a brine shrimp activity designed for students in grades 5-12 to foster authentic scientific inquiry in addition to providing an engaging and exciting avenue for student exploration. Emphasizes that inquiry should be a critical component in the science classroom. (KHR)

  4. Shrimp Ball Soup

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Press the spring onion and ginger, add 25 grams water, soak for ten minutes, filter and remove the residue.Mince the shrimp meat and add pork fat. Add Shaoxing wine, egg white, spring onion and ginger juice, salt, MSG, and cornstarch, and blend. Slice the ham, and clean the bean sprouts. Put clear stock in a wok and heat.

  5. Nutritional aspects of irradiated shrimp

    International Nuclear Information System (INIS)

    Data available in the literature on the nutritional aspects of irradiated shrimp are reviewed and the indication is that irradiation of shrimp at doses up to about 3.2 kGy does not significantly affect the levels of its protein, fat, carbohydrate and ash. There are no reports on the effect of irradiation of shrimp above 3.2 kGy on these components. Limited information available indicates that there are some minor changes in the fatty acid composition of shrimp as a result of irradiation. Irradiation also causes some changes in the amino acid composition of shrimp; similar changes occur due to canning and hot-air drying. Some of the vitamins in shrimp, such as thiamine, are lost as a result of irradiation but the loss is less extensive than in thermally processed shrimp. Protein quality of shrimp, based on the growth of rats as well as that of Tetrahymena pyriformis, is not affected by irradiation. No adverse effects attributed to irradiation were found either in short-term or long-term animal feeding tests

  6. Expression and characterisation of tiger shrimp Penaeus monodon penaeidin (mo-penaeidin) in various tissues, during early embryonic development and moulting stages.

    Science.gov (United States)

    Chiou, Tzu-Ting; Lu, Jenn-Kan; Wu, Jen-Leih; Chen, Thomas T; Ko, Chi-Fong; Chen, Jiann-Chu

    2007-01-01

    A penaeidin family, mo-penaeidin was cloned from the haemocytes of tiger shrimp Penaeus monodon using genomic polymerase chain reaction (PCR) by gene specific primers. Analysis of nucleotide sequence revealed that this mo-penaeidin consists of 1348 bp containing one intron (680 bp) and two exons (210 and 458 bp). It has an open reading frame (ORF) of 222 p, which encodes a protein of 74 amino acids including a signal peptide of 19 amino acids. The calculated molecular mass of the mature protein (55 amino acids) is 6.059 kDa with an estimated pI of 9.3. The deduced amino acid sequence of mo-penaeidin has similarity to that of penaeidin from Fenneropenaeus chinensis (73%), Farfantepenaeus paulensis (66%), Litopenaeus schmitti (53-67%), L. stylirostris (50-67%), L. setiferus (50-62%), L. vannamei (44-66%), and Marsupenaeus japonicus (33%), respectively. Phylogenetic tree analysis indicated that penaeidin (including mo-penaeidin, penaeidin, and penaeidin 5, 2, 3k, 3c1) of P. monodon is distinct from penaeidin 1, penaeidin 2, penaeidin 3 and penaeidin 4 of other penaeid shrimps. The mo-penaeidin mRNA was detected in various tissues including ovary and mandibular organ. The mo-penaeidin mRNA was present in one cell to postlarva stage with higher level at nauplius I (9h post hatching) and higher expression during the intermoult stage indicating an early innate immunity and different immunity at moulting stage. PMID:16820207

  7. SHRIMP II analysis of minerals

    International Nuclear Information System (INIS)

    Full text: Ion microprobes are specialised mass spectrometers that allow in situ isotopic and abundance measurements of chemically complex materials with a spatial resolution of 5 to 20 micrometers. The Sensitive High mass Resolution Ion Micro-Probe (SHRIMP II), an Australian built instrument, is designed to achieve high mass resolution (>5000 MRP at the 1% level) and great sensitivity, with detection limits in the ppb range for most elements in the Periodic Table. The SHRIMP ion microprobes have revolutionised geochronology and they are primarily used for U-Th-Pb dating of zircons from geological samples. The in situ analysis capability of the SHRIMP is now recognised as the best method for obtaining highly precise ages for zircons and other accessory minerals in ancient rocks with complex geological histories. The Western Australian SHRIMP II, which is located at Curtin University, provides rapid, precise measurements of isotope ratios in minerals and glasses and has been applied to a wide variety of geological problems and to a few applied physics problems. During the last three years we have expanded the number of minerals that can be used for U-Th-Pb age dating to include titanite, rutile, apatite, badellyite, perovskite and monazite. In addition to age dating the SHRIMP can be used for isotopic tracing. SHRIMP analysis provides the spatial resolution required to study S isotopes in very complex ores from many of Australia's largest ore deposits (Mt Isa, Broken Hill, Olympic Dam). Sulphur isotope ratios can be measured with a precision of 1-2 ppm in both sulphides and sulphates. In situ S isotopic compositions of galena (PbS), sphalerite (ZnS), chalcopyrite (CuS) and pyrite (FeS) has been crucial in the development of models of ore deposition. The in situ analytical capability and the high sensitivity of the SHRIMP II has allowed measurement of, diffusion profiles of REE and other trace element in melt couples, the mineral-melt partition coefficients of these

  8. Morphological Variation of Different Chinese Geographic Populations of White Shrimp (Exopalaemon modestus Heller)%中国秀丽白虾不同地理群体的形态变异

    Institute of Scientific and Technical Information of China (English)

    陈杰; 郭慧; 陈立侨; 王晓艳; 韩洁

    2007-01-01

    分别测量了300尾采自中国五个淡水湖(太湖、滇池、呼伦湖、兴凯湖和巢湖)秀丽白虾的10个形态学指标,运用三种多元统计方法对不同地理群体间的形态差异进行了比较研究.聚类分析结果表明,太湖群体和滇池群体的形态最为接近,而与兴凯湖群体、呼伦湖群体和巢湖群体的趋异程度逐渐增加.判别分析按雌、雄个体分别建立了5个群体的判别函数,其中雌体的判别准确率为80.0%~93.5%(P1),87.5%~93.5%(P2),综合判别率为90.8%;雄体的判别准确率为:80.0%~90.3%(P1),77.1%~95.2%(P2),综合判别率为87.8%.五个地理群体间的形态差异须有多项参数综合判别才能辨识.主成份分析构建的三个雌体主成份的累积贡献率为75.97%,而三个雄体主成份的累积贡献率为73.63%,五个地理群体间的形态差异主要取决于全长、尾肢长、头胸甲宽、腹部长和腹部宽等5个形态学特征.提示秀丽白虾的形态变异与其生活的水体理化因子和底质不同密切相关,推测环境因子对白虾形态特征的选择作用,使其群体表型具有可塑性.此外,不当的引种和人工养殖对滇池群体种质混杂的不良影响,需要进一步深入研究,并在选种过程中加以重视.%Morphological data of white shrimp (Exopalaemon modestus Heller) collected from five fresh water lakes in China were analyzed. Ten morphometric traits were measured from over 300 shrimps and the degree of morphological similarity among sites was calculated using multivariate analysis techniques. Eight from the eleven adjusted morphometric traits in females as well as six in males were effective to discriminate differences among five populations. Discriminant analysis revealed that over 90.8% females and 87.8% males of individuals could be classified to their sites of origin. The results of cluster analysis and principal component analysis revealed that populations from Taihu Lake and Dianchi Lake

  9. Vibrio harveyi modulated gene expression in Penaeus monodon and Fenneropenaeus indicus

    Digital Repository Service at National Institute of Oceanography (India)

    Nayak, S.

    (Saulnier et al., 2000). Major epizootics of vibriosis have been reported for P. monodon from the Indo-Pacific region, P. japonicus from Japan, and P. vannamei from Ecuador, Peru, Colombia and Central America (Lightner, 1996). For a long time many... and termination sites, 5�� and 3�� untranslated regions, promoter regions, and exon-intron splice sites. �� �� �� �� �� �� �� �� �� �� �� �� �� �� �� �� �� �� Chapter 2 Review of Literature �� �� 2.1 Introduction Shrimps are among the most economically...

  10. Polarization signals in mantis shrimps

    Science.gov (United States)

    Cronin, Thomas W.; Chiou, Tsyr-Huei; Caldwell, Roy L.; Roberts, Nicholas; Marshall, Justin

    2009-08-01

    While color signals are well known as a form of animal communication, a number of animals communicate using signals based on patterns of polarized light reflected from specialized body parts or structures. Mantis shrimps, a group of marine crustaceans, have evolved a great diversity of such signals, several of which are based on photonic structures. These include resonant scattering devices, structures based on layered dichroic molecules, and structures that use birefringent layers to produce circular polarization. Such biological polarizers operate in different spectral regions ranging from the near-UV to medium wavelengths of visible light. In addition to the structures that are specialized for signal production, the eyes of many species of mantis shrimp are adapted to detect linearly polarized light in the ultraviolet and in the green, using specialized sets of photoreceptors with oriented, dichroic visual pigments. Finally, a few mantis shrimp species produce biophotonic retarders within their photoreceptors that permit the detection of circularly polarized light and are thus the only animals known to sense this form of polarization. Mantis shrimps use polarized light in species-specific signals related to mating and territorial defense, and their means of manipulating light's polarization can inspire designs for artificial polarizers and achromatic retarders.

  11. The Brine Shrimp's Butterfly Stroke

    OpenAIRE

    Johnson, Brennan; Garrity, Deborah; Dasi, Lakshmi Prasad

    2011-01-01

    We investigate the fluid dynamics of brine shrimp larvae swimming in this gallery of fluid motion video. Time resolved particle image velocimetry was performed using nano-particles as seeding material to measure the time dependent velocity and vorticity fields. The Reynolds number of the flow was roughly 8 and the Womerseley number (ratio of periodic forcing to viscous forcing) was about 5. Vorticity dynamics reveals the formation of a vortex ring structure at the tip of each arm at the begin...

  12. Studies on luminous, Vibrio harveyi associated with shrimp culture system rearing Panaeus monodon

    Digital Repository Service at National Institute of Oceanography (India)

    Kannapiran, E.; Ravindran, J.; Chandrasekar, R.; Kalaiarasi, A.

    and V. harveyi density were low at that time. Vaseeharan and Ramasamy (2003) also reported that the growth of pathogenic V. harveyi isolated from Penaeus monodon with black gill disease was controlled by non-pathogenic B. subtilis BT23 as probiotic...: Vibrios associated with Penaeus chinensis (Crustacea: Decapoda) larvae and post-larvae in Chinese shrimp hatcheries. Aquaculture 169, 121-132 (1998). Vaseeharan, B. and P. Ramasamy: Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible...

  13. The Effects of Shrimp Gut Probiotic Bacteria on the Shrimp Larvae (Penaeus Chinensis)

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The survival rates of shrimp larvae in different stage are higher than those of control groups when probiotic bacteria strains X4B-1 and X1B-1 which are isolated from gut of adult shrimp are added into the little volume rearing sea water of shrimp larvae. The effects of probiotic bacteria are evaluated by challenge test (pathogenic bacteria Z3G2 isolated from disease shrimp larvae in the hatchery of Jimo town) and low salinity stress resistance tests on shrimp larvae, the survival rate and lengths of the shrimp larvae in the experiment are determined. Results indicate that 1. The survival rate, ability of resistant to low salinity, lengths of the delivered shrimp larvae are improved after the strains of probiotic bacteria, X4B-1 or X1B-1, are added into the rearing sea water of hatchery. 2. The addition of the probiotic bacteria could not influence the change of the bacteria number, NH3-N and COD value in the rearing sea water. 3. The probiotic bacteria used in the experiment have many enzymes such as Lipase, Amylase, Gelatinase and Lecithinase. These enzymes may help the probiotic bacteria to digest the food components fed to shrimp larvae and increase the digestive efficiency of post larvae. This may be one of the reasons why these probiotic bacteria are beneficial to the shrimp larvae.

  14. Function of shrimp STAT during WSSV infection.

    Science.gov (United States)

    Wen, Rong; Li, Fuhua; Li, Shihao; Xiang, Jianhai

    2014-06-01

    JAK/STAT signaling pathway plays key roles in the antiviral immunity of mammals, fish and insect. However, limited knowledge is known about the function of JAK/STAT signaling pathway in the antiviral immunity of shrimp although virus disease has caused severe mortality in shrimp aquaculture. In order to understand the function of JAK/STAT signaling pathway in the antiviral immunity of shrimp, dsRNA interfering technique was used to silence the expression of STAT gene in Litopenaeus vannamei, and the mortality of shrimp was detected after WSSV infection. Furthermore, the expressions of some potential target genes regulated by STAT or genes related to RNA interfering pathway were detected in STAT silenced shrimp during WSSV infection. The WSSV copy number in STAT silenced shrimp was 10(2)-10(3) copies/ng DNA which was much lower than that in the control. The mortality in STAT silenced shrimp caused by WSSV infection decreased very significantly compared to their controls. The function of STAT was verified in vitro cultured cells of hematopoietic tissue of crayfish Cherax quadricarinatus by adding specific inhibitor of STAT3(S3I-201), and the cultured cells treated with S3I-201 showed much less WSSV copy number than their controls, which further suggested that STAT might be helpful for the replication of WSSV. Expression analysis on the potential STAT target genes and genes in RNA interfering pathway provide important information for understanding the functional mechanism of STAT in antiviral immunity of shrimp. PMID:24727196

  15. Signalling by the cleaner shrimp Periclimenes longicarpus

    OpenAIRE

    Chapuis L.; Bshary R.

    2010-01-01

    Signals increase the fitness of a sender by altering the behaviour of receivers. For cooperative interactions biological market theory proposes that signalling strength may be linked to supply and demand. In this context, a recent laboratory experiment demonstrated that cleaner shrimps may advertise their service to client reef fish and that the advertisement is linked to hunger levels. We investigated signalling by the cleaner shrimp Periclimenes longicarpus in the field to test more detaile...

  16. The Brine Shrimp's Butterfly Stroke

    CERN Document Server

    Johnson, Brennan; Dasi, Lakshmi Prasad

    2011-01-01

    We investigate the fluid dynamics of brine shrimp larvae swimming in this gallery of fluid motion video. Time resolved particle image velocimetry was performed using nano-particles as seeding material to measure the time dependent velocity and vorticity fields. The Reynolds number of the flow was roughly 8 and the Womerseley number (ratio of periodic forcing to viscous forcing) was about 5. Vorticity dynamics reveals the formation of a vortex ring structure at the tip of each arm at the beginning of the power stroke. This two vortex system evolves dramatically with time as the stroke progresses. The outer circulation is noted to weaken while the inner circulation strengthens over the power stroke. The gaining strength of the inner vortex correlates with the acceleration and forward movement of the larvae.

  17. Sensory quality of frozen shrimp Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Inês Maria Barbosa Nunes Queiroga

    2014-09-01

    Full Text Available The objective of this research was to evaluate the sensory quality of the marine shrimp Litopenaeus vannamei grown and stored in a freezer. A sensory analysis consisted of the Quality Index Method (MIQ to review the raw shrimp and Descriptive Analysis (AD in cooked shrimp in samples stored for a period of 90 days, using eight previously trained panelists. Accommodation comprising shrimp filet (100-120 pieces / kg samples were subjected to freezing in liquid nitrogen (- 86 °C, Freezing Tunnel (- 35 °C and Domestic Freezer (- 18 °C. At 0, 30, 60 and 90 days of storage in these freezing systems, determination of pH, water holding capacity (WHC, weight loss during cooking (WLC, shearing force, color, total volatile bases (TVB and thiobarbituric acid reactive substances (TBARS were performed. The attributes manual firmness and softness showed better results in rapid freezing. The color parameters (a *, b * and L *, WHC and WCL were higher at 90 days of storage, no significant losses were observed. Highlighted the strength of greater shear in slowly frozen samples at 90 days, confirming the results reported by the sensory panel. At 90 days of storage, the frozen shrimp showed good sensory quality and physical and chemistry characteristics. The shelf life of this shrimp could be set at about 90 days.

  18. Effect of high intensity ultrasound on the allergenicity of shrimp

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The tropomyosin fraction of shrimp proteins is potentially responsible for allergic reaction in individuals with genetic predisposition to allergy. However, there are no efficient and safe methods to reduce its allergenicity. High intensity ultrasound is known to change the structure of proteins. This study is aimed at assessing high intensity ultrasound's effect on the allergenicity of shrimp allergen. Shrimp and purified shrimp allergen were treated with high intensity ultrasound for 30~180 min. Extracts of treated samples were analyzed by enzyme-linked immunosorbent assay (ELISA) with pool serum of shrimp allergy patients and polyclonal anti-allergen antibodies and by immunoblotting after polyacrylamide gel electrophoresis. Shrimp treated with high intensity ultrasound showed a decrease in allergenicity measured with ELISA. A linear relationship between the immune response induced by treated shrimp allergen and the applied treatment time was observed. The decrease in allergenicity was confirmed by immunoblot assays with shrimp allergic patients serum. Allergenicity of shrimp allergen extracted from treated shrimp was higher than that of purified shrimp allergen with the same treatment time. Gel-filtration HPLC was applied for analysis of shrimp allergen after treatment with high intensity ultrasound. Some fractions were appeared with increasing treatment time. The results suggested that high intensity ultrasound could be used to reduce the allergenicity of shrimp.

  19. 大蒜对印度明对虾黑鳃病原耐药菌株哈维氏弧菌抗菌活性的研究%Antibacterial activity of Allium sativum against multidrug-resistant Vibrio harveyi isolated from black gill-diseased Fenneropenaeus indicus

    Institute of Scientific and Technical Information of China (English)

    杨仲明(译)

    2012-01-01

    本研究针对大蒜提取物对印度明对虾(Fenneropenaeusindicus)黑腮病病原多重耐药哈维氏弧菌(Vibrioharveyi)体外和体内的抗菌活性进行了评价。先利用市售的几种抗生素记录致病性哈维氏弧菌的抗菌谱。然后,利用琼脂扩散法来研究大蒜对哈维氏弧菌的抗菌活性。将哈维氏弧菌接种在琼脂平板上,对每种大蒜提取物(鲜榨大蒜提取物-FsGE,冻干大蒜提取物-FDGE以及用甲醇提取的大蒜提取物-MGE)设计三种不同的浓度。结果表明,鲜榨大蒜提取物对哈维氏弧菌的抑菌活性明显优于冻干大蒜提取物和用甲醇提取的大蒜提取物。本研究分别在对虾基础饲料中添加O%(对照)、0.5%和1%浓度梯度的大蒜来评价大蒜治疗感染哈维氏弧菌的对虾的效果。结果表明,综合大蒜提取物的短期和长期防治(1%含量)的效果分析,对虾累积死亡率降低了75%。本研究探讨了鲜榨大蒜提取物替代抗生素在印度明对虾饲养防病上的优点。%The present study evaluated the in vitro and in vivo inhibitory effects of Allium sativum extracts against the multidrug- resistant Vibrio harveyi, isolated from the black gill-diseased Fenneropenaeus indicus. Initially, the antibiogram of pathogenic V. harveyi was documented for its resistance towards selected commercial antibiotics. Further, antibacterial activity of A. sativum against Vibrio harveyi was performed by the agar well diffusion method. V. harveyi culture was seeded over the agar plates, and each of the garlic extracts (freshly squeezed garlic extract-FSGE, freeze-dried garlic extract-FDGE and methanolic garlic extract-MGE) was tested at three different concentrations. FSGE showed greater inhibitory activity against V. harveyi than FDGE and MGE. Three diets of standard meal preparation for shrimp containing 0% garlic as a control, 0. 5% garlic and 1% garlic treatments were formulated to

  20. Incidence and inactivation of Listeria spp. on frozen shrimp

    Science.gov (United States)

    Foodborne illness outbreaks occasionally occur as a result of microbiologically contaminated crustaceans, including shrimp. Foodborne pathogens occasionally found on shrimp include Listeria monocytogenes, Salmonella spp., Staphylococcus aureus, and Vibrios. In this study the microbiological qualit...

  1. 2012 Economic Survey of Gulf State Shrimp License Holders

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This mail survey collected economic data on inshore commercial shrimp fishermen who held licenses to commercially harvest shrimp in state waters of the U.S. Gulf of...

  2. Development of Researches on Preservation and Storage Technology of Shrimps

    Institute of Scientific and Technical Information of China (English)

    刘海玲; 杨春莉; 杨春瑜

    2015-01-01

    With the rapid development of the world’s fisheries, shrimp consumption and trade are occupying an increasingly important position in the international aquatic products market. China is the world’s largest producer of shrimp and is also the main exporter of shrimp, however, compared with developed countries, technology level of shrimp production and processing in our country is still low at present, especially in aspect of shrimp preservation and storage techniques. So it is necessary to study various technologies and applications of sterilization and conclude the effective methods of shrimp preservation and storage. Traditional storage, chemical and biological technology, low temperature preservation, heat treatment, gas preservation and physical sterilization technology are reviewed in this paper based on shrimp preservation aiming to provide certain theoretical reference and practical basis for shelf life extension of shrimp.

  3. Shrimp Farms and Mangroves, Gulf of Fonseca

    Science.gov (United States)

    2002-01-01

    For decades, astronauts on space missions have documented land use changes around the world. In this pair of images, astronauts track the development of shrimp farming along the Honduran coastline of the Gulf of Fonseca between 1989 and 2001. Mariculture, primarily shrimp farming, has become a leading agricultural effort in Honduras. The regional transformation of large tracts of coastal swamps into shrimp farms blossomed throughout the 1990s. The top image was taken with color infrared film in 1989. Dense vegetation, like the coastal mangrove swamps and the forested slopes of Volcan Cosiguina show up as dark red. The bottom image, taken with color visible film by the crew of the most recent Space Shuttle mission in December 2001 shows that hundreds of square kilometers of coastal swamp, primarily in Honduras, have been converted to shrimp ponds. These appear as the light-colored, rectilinear land use pattern. The Honduras shrimp farms were hit hard by flooding after Hurricane Mitch in 1998, and a devastating virus in 1999-2000. It is not known how many of the ponds in this view are still functional. A vigorous debate continues about the sustainability of the shrimp farms and the impacts to the environment and coastal ecosystem due to mangrove clearing and mariculture waste production. Apart from the shrimp farms, the other prominent feature on these images is the impressive volcano Cosiguina, which erupted explosively in 1859 (the largest recorded eruption in the Western Hemisphere). Photograph STS-108-717-85 was taken in the December 2001 by the crew of Space Shuttle mission 108 using a Hasselblad camera with 250-mm lens. Photograph STS030-93-15 was taken in May 1989 using a Hasselblad camera and color infrared film. Both images are provided by the Earth Sciences and Image Analysis Laboratory at Johnson Space Center. Additional images taken by astronauts and cosmonauts can be viewed at the NASA-JSC Gateway to Astronaut Photography of Earth.

  4. Brine shrimp cytotoxic activities of Hippophae rhamnoides Linn leaves extracts

    OpenAIRE

    Javid Ali; Bashir Ahmad

    2015-01-01

    Objective: To evaluate brine shrimp lethality assay of solvent extracts (aqueous, methanol, ethanol, acetone, ethyl acetate, chloroform and n-hexane) of Hippophae rhamnoides (H. rhamnoides) leaves. Methods: Brine shrimp cytotoxicity assay was used to assess the cytotoxic potential of H. rhamnoides leaves extracts. Three vials for concentration of each extract were made and 10 shrimps per vial (30 shrimps per dilution) were transferred to specific concentration of each extrac...

  5. Gamma Irradiation for the Inhibition of Shrimp (Penaeus aztecus) Allergy

    International Nuclear Information System (INIS)

    Food irradiation technology was conducted to reduce shrimp allergy. The experiment was designated in 3 portions as follows; A, the irradiation of raw shrimp; B the irradiation of shrimp and then cooking; and C, cooking the shrimp and then irradiation. Gamma irradiation was done with doses of 1, 3, 5, 7, 10 kGy. A shrimp sarcoplasmic protein solution (SSPS) and a myofibrillar protein solution (SMPS) were prepared from A portion. Cooked shrimp protein solutions were also prepared from B and C portions. The binding abilities of the shrimp allergic patients' IgE and mouse monoclonal Ab 4.9.5 (mAb 4.9.5), produced to the shrimp heat-stable protein, to each sample solution were determined by ELISA. Binding abilities of patients' IgE and mAb 4.9.5 to irradiated shrimp fractions were dose-dependently reduced. The cooking treatment after irradiation was more effective than the irradiation treatment after cooking in the reduction of the binding abilities of IgE and IgG. SDS-PAGE was performed to compare irradiated shrimp proteins with non-irradiated shrimp proteins. SDS-PAGE showed that no bands were changed by gamma irradiation. The results indicated that food irradiation with an adequate dose can be reduce allergenicity of shrimp

  6. The efficacy testing of irradiated shrimp paste

    International Nuclear Information System (INIS)

    Two lots of shrimp paste from commercial source in Samutsakhon were irradiated at a recommended minimum dose of 6 kGy using a J S 8900 cobalt-60 carrier gamma irradiator of Thai Irradiation Center in Patum Thani. Red Perspex dosimeter were used to measure the absorbed dose throughout the product with emphasis on the region of minimum and maximum absorbed dose. This way, it was aimed to compare the dose effects of gamma irradiation on the microbiological, chemical and sensory quality of shrimp paste. The results indicated that the shrimp paste received minimum and maximum absorbed dose of 6.85 and 12.83 kGy with dose uniformity ratio of 1.87 . Throughput rate is 468 kilogram per hour. The microbiological load of shrimp paste was rather high resulting in not compliance with Thai industrial standard 1080-2535. Irradiation at 6.8 kGy reduced total viable bacterial count by one log cycle. Although the irradiated product was organoleptic ally acceptable and could be kept for 16 months at room temperature, mold and Clostridium perfringens were still present in some samples after irradiation and during prolonged storage in amount that exceeds the limitation of Thai industrial standard. Chemical properties such as p H, moisture and sodium chloride content of irradiated shrimp paste were not significantly changed after irradiation

  7. Biological treatment of shrimp production wastewater.

    Science.gov (United States)

    Boopathy, Raj

    2009-07-01

    Over the last few decades, there has been an increase in consumer demand for shrimp, which has resulted in its worldwide aquaculture production. In the United States, the stringent enforcement of environmental regulations encourages shrimp farmers to develop new technologies, such as recirculating raceway systems. This is a zero-water exchange system capable of producing high-density shrimp yields. The system also produces wastewater characterized by high levels of ammonia, nitrate, nitrite, and organic carbon, which make waste management costs prohibitive. Shrimp farmers have a great need for a waste management method that is effective and economical. One such method is the sequencing batch reactor (SBR). A SBR is a variation of the activated sludge biological treatment process. This process uses multiple steps in the same reactor to take the place of multiple reactors in a conventional treatment system. The SBR accomplishes equalization, aeration, and clarification in a timed sequence in a single reactor system. This is achieved through reactor operation in sequences, which includes fill, react, settle, decant, and idle. A laboratory scale SBR was successfully operated using shrimp aquaculture wastewater. The wastewater contained high concentrations of carbon and nitrogen. By operating the reactors sequentially, namely, aerobic and anoxic modes, nitrification and denitrification were achieved as well as removal of carbon. Ammonia in the waste was nitrified within 4 days. The denitrification of nitrate was achieved by the anoxic process, and 100% removal of nitrate was observed within 15 days of reactor operation. PMID:19396482

  8. Determination of Biogenic Amines in Different Shrimp Species for Export

    International Nuclear Information System (INIS)

    This study is part of the project on the ''Quality Assurance of Different Shrimp Species for Export''. Local shrimp samples were collected from Ministry of Livestock and Fisheries and various private enterprises. Contents of biogenic amines were determined by using benzoyl chloride derivatization method with HPLC (reverse phase high performance liquid chromatography). Based on the biogenic amines, quality index of shrimps were correlated with freshness index so that the grade of shrimp samples can be classified as excellent, good, and acceptable. All sizes of shrimps such as extra large, large, medium were found to excceptable respectively

  9. Improvement of bacteriological quality of frozen shrimp by gamma radiation

    International Nuclear Information System (INIS)

    The possible use of gamma irradiation at doses of 2 and 3 kGy to improve bacteriological quality of frozen shrimp was investigated. The effects of gamma irradiation on salmonella viability in frozen shrimp and on sensory quality of frozen shrimp were also evaluated. Treatment of frozen shrimp at 3 kGy reduced bacterial load by 2 log cycles and eliminated Enterobacteriaceae, E. coli, V. parahaemolyticus, and S. aureus. Coliform was still present in a 3 kGy treated samples but in a very small percentage and the count was less than 10 cell/g. Salmonella was not detected in either irradiated or non-irradiated frozen shrimp. A difference in radiation resistance was noted among five salmonella serotypes tested. S. lexington was the most resistant and S. anatum was the least resistant. Salmonella resistance was higher in frozen inoculated shrimp than in refrigerated inoculated shrimp. A dose of at least 4.2 kGy is required for a seven log cycle reduction of salmonella contamination in frozen shrimp. Off-flavors were produced in frozen shrimp irradiated at 4.2 kGy but diminished during subsequent frozen storage. However, irradiated frozen shrimp was of acceptable quality for at least four months. Dosage at 3 kGy appeared to be sufficient for improving bacteriological quality of frozen shrimp

  10. Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish

    Institute of Scientific and Technical Information of China (English)

    WANG Dongdong; LI Fuhua; LI Shihao; WEN Rong; XIANG Jianhai

    2012-01-01

    Antimicrobial peptides (AMPs),as key immune effectors,play important roles in the innate immune system of invertebrates.Different types of AMPs,including Penaeidin,Crustin,ALF (antilipopolysaccharide factor) have been identified in different penaeid shrimp; however,systematic analyses on the function of different AMPs in shrimp responsive to different types of bacteria are very limited.In this study,we analyzed the expression profiles of AMPs in the Chinese shrimps,Fenneropenaeus chinensis,simultaneously by real-time RT-PCR (reverse transcription-polymerase chain reaction) when shrimp were challenged with Micrococcus lysodeikticus (Gram-positive,G+) or Vibrio anguillarium (Gram-negative,G).Different AMPs showed different expression profiles when shrimp were injected with one type of bacterium,and one AMP also showed different expression profiles when shrimp were challenged with different bacteria.Furthermore,the expression of these AMPs showed temporal expression profiles,suggesting that different AMPs function coordinately in bacteria-infected shrimp.An RNA interference approach was used to study the function of the Relish transcription factor in regulating the transcription of different AM Ps.The current study showed that Relish could regulate the transcription of different AMPs in shrimp.Differential expression profiles of AMPs in shrimp injected with different types of bacteria indicated that a complicated antimicrobial response network existed in shrimp.These data contribute to our understanding of immunity in shrimp and may provide a strategy for the control of disease in shrimp.

  11. Expression profiles of antimicrobial peptides (AMPs) and their regulation by Relish

    Science.gov (United States)

    Wang, Dongdong; Li, Fuhua; Li, Shihao; Wen, Rong; Xiang, Jianhai

    2012-07-01

    Antimicrobial peptides (AMPs), as key immune effectors, play important roles in the innate immune system of invertebrates. Different types of AMPs, including Penaeidin, Crustin, ALF (antilipopolysaccharide factor) have been identified in different penaeid shrimp; however, systematic analyses on the function of different AMPs in shrimp responsive to different types of bacteria are very limited. In this study, we analyzed the expression profiles of AMPs in the Chinese shrimps, Fenneropenaeus chinensis, simultaneously by real-time RT-PCR (reverse transcription-polymerase chain reaction) when shrimp were challenged with Micrococcus lysodeikticus (Gram-positive, G+) or Vibrio anguillarium (Gram-negative, G-). Different AMPs showed different expression profiles when shrimp were injected with one type of bacterium, and one AMP also showed different expression profiles when shrimp were challenged with different bacteria. Furthermore, the expression of these AMPs showed temporal expression profiles, suggesting that different AMPs function coordinately in bacteria-infected shrimp. An RNA interference approach was used to study the function of the Relish transcription factor in regulating the transcription of different AMPs. The current study showed that Relish could regulate the transcription of different AMPs in shrimp. Differential expression profiles of AMPs in shrimp injected with different types of bacteria indicated that a complicated antimicrobial response network existed in shrimp. These data contribute to our understanding of immunity in shrimp and may provide a strategy for the control of disease in shrimp.

  12. CO₂ efflux from shrimp ponds in Indonesia.

    Directory of Open Access Journals (Sweden)

    Frida Sidik

    Full Text Available The conversion of mangrove forest to aquaculture ponds has been increasing in recent decades. One of major concerns of this habitat loss is the release of stored 'blue' carbon from mangrove soils to the atmosphere. In this study, we assessed carbon dioxide (CO₂ efflux from soil in intensive shrimp ponds in Bali, Indonesia. We measured CO₂ efflux from the floors and walls of shrimp ponds. Rates of CO₂ efflux within shrimp ponds were 4.37 kg CO₂ m⁻² y⁻¹ from the walls and 1.60 kg CO₂ m⁻² y⁻¹ from the floors. Combining our findings with published data of aquaculture land use in Indonesia, we estimated that shrimp ponds in this region result in CO₂ emissions to the atmosphere between 5.76 and 13.95 Tg y⁻¹. The results indicate that conversion of mangrove forests to aquaculture ponds contributes to greenhouse gas emissions that are comparable to peat forest conversion to other land uses in Indonesia. Higher magnitudes of CO₂ emission may be released to atmosphere where ponds are constructed in newly cleared mangrove forests. This study indicates the need for incentives that can meet the target of aquaculture industry without expanding the converted mangrove areas, which will lead to increased CO₂ released to atmosphere.

  13. Neoliberalism and shrimp industry in Ecuador

    Directory of Open Access Journals (Sweden)

    Nadia Romero Salgado

    2014-04-01

    Full Text Available Analysis of the effects of the shrimp industry development in the mangrove ecosystem, the socio-environmental conflicts generated and its relationship with neoliberalism and the financial crisis of 1999 in Ecuador. After a review of the importance of the mangrove ecosystem, the stages of the shrimp expansion, its promoters, the mangrove deforestation and the socio-environmental effects caused, I will analyze the shrimp crisis, its parallels with the financial crisis of 1999 and its subsequent recovery. I will show that the shrimp industry expanded in mangrove areas in order to reduce costs, even breaking the law and creating environmental degradation, vulnerability of the costs and loss of natural resources, based on the exploitation and privatization of a public good. This created unemployment, migration and impoverishment to local populations and costs that the State will have to assume. Therefore, it is a process of “accumulation by dispossession” characteristic of neoliberalism.

  14. How snapping shrimp snap: through cavitating bubbles

    NARCIS (Netherlands)

    Versluis, Michel; Schmitz, Barbara; Heydt, von der Anna; Lohse, Detlef

    2000-01-01

    The snapping shrimp (Alpheus heterochaelis) produces a loud snapping sound by an extremely rapid closure of its snapper claw. One of the effects of the snapping is to stun or kill prey animals. During the rapid snapper claw closure, a high-velocity water jet is emitted from the claw with a speed exc

  15. On the Sound of Snapping Shrimp

    NARCIS (Netherlands)

    Versluis, Michel; Heydt, von der Anna; Lohse, Detlef; Schmitz, Barbara

    2001-01-01

    Snapping shrimp produce a snapping sound by an extremely rapid closure of their snapper claw. Source levels reported for Alpheus heterochaelis are as high as 220 dB (peak-to-peak) re. 1 µPa at 1 m distance. The loud snap has been attributed to the mechanical contact made when the snapper claw contra

  16. Is the Dutch shrimp fishery sustainable?

    NARCIS (Netherlands)

    Welleman, H.C.; Daan, N.

    2001-01-01

    The fishery of the brown shrimp (Crangon crangon LINNEAUS 1758) is a widespread human activity in the coastal zone. Yet management of this fishery has never been implemented. The question is raised whether an uncontrolled fishery is sustainable or the conceivable ecological stress results in recruit

  17. Solar Powered Automatic Shrimp Feeding System

    Directory of Open Access Journals (Sweden)

    Dindo T. Ani

    2015-12-01

    Full Text Available - Automatic system has brought many revolutions in the existing technologies. One among the technologies, which has greater developments, is the solar powered automatic shrimp feeding system. For instance, the solar power which is a renewable energy can be an alternative solution to energy crisis and basically reducing man power by using it in an automatic manner. The researchers believe an automatic shrimp feeding system may help solve problems on manual feeding operations. The project study aimed to design and develop a solar powered automatic shrimp feeding system. It specifically sought to prepare the design specifications of the project, to determine the methods of fabrication and assembly, and to test the response time of the automatic shrimp feeding system. The researchers designed and developed an automatic system which utilizes a 10 hour timer to be set in intervals preferred by the user and will undergo a continuous process. The magnetic contactor acts as a switch connected to the 10 hour timer which controls the activation or termination of electrical loads and powered by means of a solar panel outputting electrical power, and a rechargeable battery in electrical communication with the solar panel for storing the power. By undergoing through series of testing, the components of the modified system were proven functional and were operating within the desired output. It was recommended that the timer to be used should be tested to avoid malfunction and achieve the fully automatic system and that the system may be improved to handle changes in scope of the project.

  18. Key Performance Characteristics of Organic Shrimp Aquaculture in Southwest Bangladesh

    Directory of Open Access Journals (Sweden)

    Christian Reinhard Vogl

    2012-05-01

    Full Text Available In Bangladesh, black tiger shrimp (Penaeus monodon; Fabricius, 1798 aquaculture has come to be one of the most important sectors in both the rural and national economies. Likewise, organic shrimp aquaculture has emerged as an alternative farming enterprise for farmers especially in the southwestern districts of Bangladesh. The present study aims to show key performance characteristics of organic shrimp farmers and farming in a prototypical shrimp farming area in Bangladesh. Data was collected in 2009 from organic shrimp farmers in the Kaligonj and Shyamnagar sub-districts through questionnaire interviews, transect walks and focus group discussions. The mean productivity of organic shrimp farming in the area is 320 kg ha−1 yr−1 (ranging from 120 to 711 kg ha−1year−1. Organic farmers are more likely to have a higher monthly income and less aquaculture experience. Moreover, suitable landholdings and classified labor distribution have been found to play an important role in the development of organic shrimp aquaculture. The most common assets of organic shrimp aquaculture are high yield, low production cost, available post larvae and high market prices. Small business farmers are likely to earn more income benefits from organic shrimp aquaculture than their larger-scale counterparts. Finally, the paper suggests that more research is needed to stimulate the success of organic shrimp aquaculture.

  19. Shrimp aquaculture in low salinity water feeded with worm flavor

    Directory of Open Access Journals (Sweden)

    Wenceslao Valenzuela Quiñónez

    2012-09-01

    Full Text Available Shrimp aquaculture in Sinaloa is one of the top economic enterprises, generating many jobs and earns significant incomes every year. Shrimp feed is an essential part of maintaining healthy production. In this initial approach of shrimp growth in low salinity water, were tested two formulas of animal protein composed of 40% (APL1 and 20% (APL2 worm protein, a commercial diet, and no supplementary feed. Physicochemical parameters did not have a direct influence in shrimpbehavior. After six weeks of experimentation, shrimp fed with commercial diet had a weight gain 20% higher than those feed with worm protein. There were no significantly differences between sizes with respect to 40% animal protein and 20% animal protein with the commercial diet (P  0.05. However, shrimp fed worm protein had lower mortality. The use of worm protein could be an option to maintain a high quantity of shrimp reared in low salinity waters.

  20. Development of the brine shrimp Artemia is accelerated during spaceflight

    Science.gov (United States)

    Spooner, B. S.; Metcalf, J.; DeBell, L.; Paulsen, A.; Noren, W.; Guikema, J. A.

    1994-01-01

    Developmentally arrested brine shrimp cysts have been reactivated during orbital spaceflight on two different Space Shuttle missions (STS-50 and STS-54), and their subsequent development has been compared with that of simultaneously reactivated ground controls. Flight and control brine shrimp do not significantly differ with respect to hatching rates or larval morphology at the scanning and transmission EM levels. A small percentage of the flight larvae had defective nauplier eye development, but the observation was not statistically significant. However, in three different experiments on two different flights, involving a total of 232 larvae that developed in space, a highly significant difference in degree of flight to control development was found. By as early as 2.25 days after reactivation of development, spaceflight brine shrimp were accelerated, by a full instar, over ground control brine shrimp. Although developing more rapidly, flight shrimp grew as long as control shrimp at each developmental instar or stage.

  1. The impact of shrimp farming on mangrove ecosystems

    DEFF Research Database (Denmark)

    Ashton, Elizabeth Clare

    2008-01-01

    . Policy to position shrimp farms behind mangroves can be effective but also requires good institutional capacity and coordination, effective enforcement, incentives, land tenure and participation of all stakeholders for success. Better management practices have been identified which reduce impacts......Farmed shrimp production and value continue to increase with Asia producing the global majority of shrimp and the USA, Japan and Europe being the main importers. Shrimp farming systems are very diverse in their management, size and impacts. There are many causes for mangrove loss but the conversion...... of mangroves to shrimp farms has caused considerable attention. The major issues of shrimp farming include the loss of important ecological and socio-economic functions of mangrove ecosystems, changes in hydrology, salinization, introduction of non-native species and diseases, pollution from effluents...

  2. Quantitative role of shrimp fecal bacteria in organic matter fluxes in a recirculating shrimp aquaculture system.

    Science.gov (United States)

    Beardsley, Christine; Moss, Shaun; Malfatti, Francesca; Azam, Farooq

    2011-07-01

    Microorganisms play integral roles in the cycling of carbon (C) and nitrogen (N) in recirculating aquaculture systems (RAS) for fish and shellfish production. We quantified the pathways of shrimp fecal bacterial activities and their role in C- and N-flux partitioning relevant to culturing Pacific white shrimp, Penaeus (Litopenaeus) vannamei, in RAS. Freshly produced feces from P. vannamei contained 0.6-7 × 10(10) bacteria g(-1) dry wt belonging to Bacteroidetes (7%), Alphaproteobacteria (4%), and, within the Gammaproteobacteria, almost exclusively to the genus Vibrio (61%). Because of partial disintegration of the feces (up to 27% within 12 h), the experimental seawater became inoculated with fecal bacteria. Bacteria grew rapidly in the feces and in the seawater, and exhibited high levels of aminopeptidase, chitinase, chitobiase, alkaline phosphatase, α- and β-glucosidase, and lipase activities. Moreover, fecal bacteria enriched the protein content of the feces within 12 h, potentially enriching the feces for the coprophagous shrimp. The bacterial turnover time was much faster in feces (1-10 h) than in mature RAS water (350 h). Thus, shrimp fecal bacteria not only inoculate RAS water but also contribute to bacterial abundance and productivity, and regulate system processes important for shrimp health. PMID:21426366

  3. Shrimp aquaculture in low salinity water feeded with worm flavor

    OpenAIRE

    Wenceslao Valenzuela Quiñónez; Gerardo Rodríguez-Quiroz; Héctor Manuel Esparza Leal; Eusebio Nava Pérez

    2012-01-01

    Shrimp aquaculture in Sinaloa is one of the top economic enterprises, generating many jobs and earns significant incomes every year. Shrimp feed is an essential part of maintaining healthy production. In this initial approach of shrimp growth in low salinity water, were tested two formulas of animal protein composed of 40% (APL1) and 20% (APL2) worm protein, a commercial diet, and no supplementary feed. Physicochemical parameters did not have a direct influence in shrimpbehavior. After six we...

  4. Immunological-based assays for specific detection of shrimp viruses

    OpenAIRE

    Chaivisuthangkura, Parin; Longyant, Siwaporn; Sithigorngul, Paisarn

    2014-01-01

    Among shrimp viral pathogens, white spot syndrome virus (WSSV) and yellow head virus (YHV) are the most lethal agents, causing serious problems for both the whiteleg shrimp, Penaeus (Litopenaeus) vannamei, and the black tiger shrimp, Penaeus (Penaeus) monodon. Another important virus that infects P. vannamei is infectious myonecrosis virus (IMNV), which induces the white discoloration of affected muscle. In the cases of taura syndrome virus and Penaeus stylirostris densovirus (PstDNV; formerl...

  5. Optimization of carotenoids extraction from Penaeus semisulcatus shrimp wastes

    OpenAIRE

    Gholamreza jahed Khaniki; Parisa Sadighara; Ramin Nabizadeh Nodehi; Mahmood Alimohammadi; Naiema Vakili Saatloo

    2013-01-01

    Objective: To find effective method for carotenoids extraction from shrimp waste which is one of the important sources of natural carotenoids and produced in large quantities in Iran. Methods: Two methods of carotenoids extraction, enzymatic and alkaline (NaOH 1 normal) treatment, were assayed. About 5 g of gritted shrimp wastes were used at each stage. For alkaline treatment, sodium hydroxide were added to shrimp waste. After 48 h, the mixture was filtered and centrifuged. ...

  6. Immune defence White Spot Syndrome Virus infected shrimp, Penaeus monodon

    OpenAIRE

    Arts, J.A.J.

    2006-01-01

    White spot syndrome virus (WSSV) is the most important viral pathogen of cultured penaeid shrimp worldwide. Since the initial discovery of the virus inTaiwanin 1992, it has spread to shrimp farming regions in Southeast Asia, theAmericas, Europe and theMiddle Eastcausing major economic losses. The virus has a wide host range among crustaceans and induces distinctive clinical signs (white spots)on the inner surface of the exoskeletonof penaeid shrimps.Limited data is available about the immune ...

  7. Feed palatability and the alternative protein sources in shrimp feed

    OpenAIRE

    Chutima Tantikitti

    2014-01-01

    Feed palatability in carnivorous aquaculture species, shrimps in particular, has been crucially related to the presence of compounds acting as attractants that are commonly associated with the prey components under wild conditions. Thus a nutritionally adequate and organoleptically-pleasing diet is essential to achieve satisfactory intake and growth in shrimps. Historically, fishmeal has been an essential dietary component of intensive shrimp cultures because of its nutrient composit...

  8. STRATEGIES FOR DEVELOPING SUSTAINABLE AND COMPETITIVE CLUSTER FOR SHRIMP INDUSTRY

    OpenAIRE

    Anas M. Fauzi; R.Dikky Indrawan; Alim Setiawan Slamet; Farida Ratna Dewi; Lindawati Kartika; Firmansjah S

    2012-01-01

    Kampung Vannamei as shrimp cluster is being developed since 2004 by PT CP Prima, tbk Surabaya through Shrimp Culture Health Management transformation technology to several traditional farmers in Gresik, Lamongan, Tuban, and Madura areas. The research objectives aims to identify and mapping of stakeholder, to analyze interaction of stakeholders, to formulate strategy from internal and external environment factors and to set priority on strategy to develop sustainable and competitive shrimp clu...

  9. Differential gene expression analysis based on expressed sequence tags(EST) from different tissues of Fenneropenaeus chinensis%基于中国明对虾不同组织EST数据的基因表达差异分析

    Institute of Scientific and Technical Information of China (English)

    隗健凯; 柳承璋; 张晓军; 王兵; 董波; 李富花; 相建海

    2013-01-01

    Bioinformatics analysis was conducted based on the acquired expressed sequence tags(ESTs) data from different tissues of Fenneropenaeus chinensis.A total of 10 446,2 690,1 067 and 1 282 original ESTs were obtained from cephalothorax,blood,eyestalk,and ovary of adult F.chinensis respectively.By clustering and assembling,3 454,1 053,406 and 544 unigenes were generated,and then annotated by searching in NR,GO,KEGG databases.The tissue specific transcripts were identified through sequence homology analysis and classified by GO annotation.The result indicated that specific transcripts in blood group were specifically enriched in the GO term of virion part,locomotion,rhythmic process,cell killing and multi-organism process.Specific transcripts in eyestalk group were specifically enriched in the GO term of molecular transducer activity,response to stimulus,multicellular organismal process,pigmentation and biological regulation.Specific transcripts in ovary group were specifically enriched in the GO term of nutrient reservoir activity,reproduction,anatomical structure formation,transporter activity and establishment of localization.We also analyzed the highly expressed genes in each tissue according to the number of ESTs of each unigene.The result indicated that genes encoding peritrophin,elongation factor 1-alpha,thrombospondin and arginine kinase were widespread and highly expressed in tissues,suggesting that they were involved in a variety of important biological processes in shrimp.In addition,genes encoding penaeidin,cytochrome c oxidase and 14-3-3-like protein were highly expressed in blood group and genes encoding arrestin and rhodopsin were highly expressed in eyestalk group.For understanding the expressions of common genes,we analyzed genes related to peritrophin and peroxiredoxin,revealing that genes of peritrophin in F.chinensis were multiform and highly expressed.This result may provide reference for further functional study of peritrophin.%对前期获得的

  10. A review of the chemical aspects of irradiated shrimp

    International Nuclear Information System (INIS)

    The literature was reviewed for information on the chemical aspects of irradiated shrimp. Low-dose irradiation can effectively control spoilage and pathogenic organisms and extend the refrigerated shelf life of shrimp. Radiation-induced increases in black discoloration occur in some species during storage, but can be minimized by proper blanching. Some loss of the characteristic pink color occurs at doses above 2.5 kGy, and irradiation off-odors occur above 1.5 kGy. These changes can be minimized by irradiating the shrimp when they are frozen. No significant change in protein, fat, carbohydrate, and ash content occurs as a result of low-dose irradiation. Low-dose irradiation does not produce any detectable change in the levels of volatile carbonyl compounds; irradiation at 8 kGy results in a transient increase in the total volatile compounds during subsequent storage in ice, but the increase is higher in the unirradiated shrimp. Radiation-induced changes in the fatty acid composition of shrimp are small. Some minor changes in the amino acid composition occur in irradiated shrimp; similar changes occur due to other processes such as canning and drying in hot air. Some vitamins in shrimp such as thiamine are affected by irradiation. But the loss is less extensive than in thermally processed shrimp. The protein efficiency ratio is not affected by irradiation of shrimp, and no adverse effects attributed to irradiation were found in animal feeding studies

  11. 丁香酚对中国对虾幼虾麻醉效果的初步研究%Studies on Anesthetic Effect of Eugenol on Juvenile Fenneropenaeus chinensis

    Institute of Scientific and Technical Information of China (English)

    黄雪芹; 孔杰; 张天时; 罗坤; 赖光艳

    2008-01-01

    [Objective] The aim of the study is to seek a good anesthetic to Fenneropenaeus chinensis. [Method] The anesthetic effect of eugenol to juvenile Fenneropenaeus chinensis was investigated. [Result] The juveniles could be effectively anaesthetized by 50-400 mg/L eugenol aqueous solution with temperature of 24 ℃. Within the concentration range of 50-400 mg/L, the increase of the eugenol concentration could shorten the time required for anesthesia, meanwhile could prolong the time for recovery. The recovered rate of prawn reached 100% when the eugenol concentration was lower than 200 mg/L, while the recovered rate of prawn was just 66.67% when the eugenol concentration was higher than 400 mg/L. The survival rate of prawns in test group was 100% from the observation of three consecutive days. For the specific dose, the anesthetic effect enhanced with the increase of water temperature (18-27℃). [Conclusion] Eugenol is a safe and efficient anesthetics that can be applied in genetic breeding of prawn.

  12. Vitamin requirements of juvenile penaeid shrimp

    OpenAIRE

    Conklin, D.

    1989-01-01

    The results of supplementing crustacean feeds with vitamins are examined specifically from the standpoint of shrimp culture. Micro-nutrients selected for discussion include: water-soluble vitamins of the B-complex, choline and inositol, vitamin C and the fat-soluble group of vitamins: A, D, E and K. Ways in which utilization of vitamins and ultimately dietary demand are altered by physiological state, conditions of culture, as well as factors which impact on feed levels, are explored.

  13. Dead shrimp blues: a global assessment of extinction risk in freshwater shrimps (Crustacea: Decapoda: Caridea).

    Science.gov (United States)

    De Grave, Sammy; Smith, Kevin G; Adeler, Nils A; Allen, Dave J; Alvarez, Fernando; Anker, Arthur; Cai, Yixiong; Carrizo, Savrina F; Klotz, Werner; Mantelatto, Fernando L; Page, Timothy J; Shy, Jhy-Yun; Villalobos, José Luis; Wowor, Daisy

    2015-01-01

    We present the first global assessment of extinction risk for a major group of freshwater invertebrates, caridean shrimps. The risk of extinction for all 763 species was assessed using the IUCN Red List criteria that include geographic ranges, habitats, ecology and past and present threats. The Indo-Malayan region holds over half of global species diversity, with a peak in Indo-China and southern China. Shrimps primarily inhabit flowing water; however, a significant subterranean component is present, which is more threatened than the surface fauna. Two species are extinct with a further 10 possibly extinct, and almost one third of species are either threatened or Near Threatened (NT). Threats to freshwater shrimps include agricultural and urban pollution impact over two-thirds of threatened and NT species. Invasive species and climate change have the greatest overall impact of all threats (based on combined timing, scope and severity of threats). PMID:25807292

  14. Dead shrimp blues: a global assessment of extinction risk in freshwater shrimps (Crustacea: Decapoda: Caridea.

    Directory of Open Access Journals (Sweden)

    Sammy De Grave

    Full Text Available We present the first global assessment of extinction risk for a major group of freshwater invertebrates, caridean shrimps. The risk of extinction for all 763 species was assessed using the IUCN Red List criteria that include geographic ranges, habitats, ecology and past and present threats. The Indo-Malayan region holds over half of global species diversity, with a peak in Indo-China and southern China. Shrimps primarily inhabit flowing water; however, a significant subterranean component is present, which is more threatened than the surface fauna. Two species are extinct with a further 10 possibly extinct, and almost one third of species are either threatened or Near Threatened (NT. Threats to freshwater shrimps include agricultural and urban pollution impact over two-thirds of threatened and NT species. Invasive species and climate change have the greatest overall impact of all threats (based on combined timing, scope and severity of threats.

  15. Dietary supplementation of probiotic Bacillus PC465 isolated from the gut of Fenneropenaeus chinensis improves the health status and resistance of Litopenaeus vannamei against white spot syndrome virus.

    Science.gov (United States)

    Chai, Peng-Cheng; Song, Xiao-Ling; Chen, Guo-Fu; Xu, Hua; Huang, Jie

    2016-07-01

    This study conducted a 30-day feeding trial and a subsequent 20-day anti-virus infection trial to determine the effects of probiotic Bacillus PC465 on the growth, health status, and disease resistance of Litopenaeus vannamei. Shrimp samples were fed with three practical diets prepared from shrimp feed containing varying probiotic doses [0 (control), 10(7), and 10(9) CFU g(-1)]. Probiotic supplementation significantly increased the weight gain and survival of L. vannamei (p digestive enzymes, such as amylase, protease, and lipase, in the shrimp mid-gut significantly increased in the probiotic-fed groups on days 15 and 30, except lipase on day 30. The influence of 10(9) CFU g(-1) on enzyme activities was also greater than that of 10(7) CFU g(-1). Scanning electron microscopy revealed folds and large ravines across the interior surface of the mid-gut, and the number of these folds and ravines increased significantly after the probiotic was administered. The probiotic treatment significantly (p digestion, and nutrient absorption of L. vannamei. Probiotic treatment also enhances the microbial structures in the gut, promotes the immune status of shrimp, and provides protection against viral infection. The supplementation with 10(9) CFU g(-1) can also improve the growth and survival of L. vannamei. PMID:27177431

  16. Closed recirculating system for shrimp-mollusk polyculture

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    This paper deals with a new system of aquaculture, i.e., a closed recirculating system for shrimp-mollusk polyculture. The culture system consisted of several shrimp ponds, a mollusk water-purifying pond and a reservoir. During the production cycle, water circulated between the shrimp and mollusk ponds, and the reservoir compensated for water loss from seepage and evaporation. Constricted tagelus, Sinonovacula constricta, was selected as the cultured mollusk, and Pacific white shrimp, Litopenaeus vannamei, as the cultured shrimp. The main managing measures during the production cycle were: setting and using the aerators; introducing the probiotic products timely into the shrimp ponds; adopting a "pen-closing" method for controlling shrimp viral epidemics; setting the flow diversion barriers in the mollusk pond to keep the circulating water flowing through the pond along a sine-like curve and serve as substrate for biofilm; no direct feeding was necessary for the cultured mollusk until the co-cultured shrimp was harvested; natural foods in the water from the shrimp ponds was used for their foods. Two sets of the system were used in the experiment in 2002 and satisfactory results were achieved. The average yield of the shrimp was 11 943.5 kg/hm2, and that of the mollusk was 16 965kg/hm2. After converting the mollusk yield into shrimp yield at their market price ratio, the food coefficient of the entire system averaged at as low as 0.81. The water quality in the ponds was maintained at a desirable level and no viral epidemics were discovered during the production cycle.

  17. Closed recirculating system for shrimp-mollusk polyculture

    Science.gov (United States)

    Wu, Xiongfei; Zhao, Zhidong; Li, Deshang; Chang, Kangmei; Tong, Zhuanshang; Si, Liegang; Xu, Kaichong; Ge, Bailin

    2005-12-01

    This paper deals with a new system of aquaculture, i.e., a closed recirculating system for shrimp-mollusk polyculture. The culture system consisted of several shrimp ponds, a mollusk water-purifying pond and a reservoir. During the production cycle, water circulated between the shrimp and mollusk ponds, and the reservoir compensated for water loss from seepage and evaporation. Constricted tagelus, Sinonovacula constricta, was selected as the cultured mollusk, and Pacific white shrimp, Litopenaeus vannamei, as the cultured shrimp. The main managing measures during the production cycle were: setting and using the aerators; introducting the probiotic products timely into the shrimp ponds; adopting a “pen-closing” method for controlling shrimp viral epidemics; setting the flow diversion barriers in the mollusk pond to keep the circulating water flowing through the pond along a sine-like curve and serve as substrate for biofilm; no direct feeding was necessary for the cultured mollusk until the co-cultured shrimp was harvested; natural foods in the water from the shrimp ponds was used for their foods. Two sets of the system were used in the experiment in 2002 and satisfactory results were achieved. The average yield of the shrimp was 11 943.5 kg/hm2, and that of the mollusk was 16 965 kg/hm2. After converting the mollusk yield into shrimp yield at their market price ratio, the food coefficient of the entire system averaged at as low as 0.81. The water quality in the ponds was maintained at a desirable level and no viral epidemics were discovered during the production cycle.

  18. Bacterial dynamics in intestines of the black tiger shrimp and the Pacific white shrimp during Vibrio harveyi exposure.

    Science.gov (United States)

    Rungrassamee, Wanilada; Klanchui, Amornpan; Maibunkaew, Sawarot; Karoonuthaisiri, Nitsara

    2016-01-01

    The intestinal microbiota play important roles in health of their host, contributing to maintaining the balance and resilience against pathogen. To investigate effects of pathogen to intestinal microbiota, the bacterial dynamics upon a shrimp pathogen, Vibrio harveyi, exposures were determined in two economically important shrimp species; the black tiger shrimp (BT) and the Pacific white shrimp (PW). Both shrimp species were reared under the same diet and environmental conditions. Shrimp survival rates after the V. harveyi exposure revealed that the PW shrimp had a higher resistance to the pathogen than the BT shrimp. The intestinal bacterial profiles were determined by denaturing gradient gel electrophoresis (DGGE) and barcoded pyrosequencing of the 16S rRNA sequences under no pathogen challenge control and under pathogenic V. harveyi challenge. The DGGE profiles showed that the presence of V. harveyi altered the intestinal bacterial patterns in comparison to the control in BT and PW intestines. This implies that bacterial balance in shrimp intestines was disrupted in the presence of V. harveyi. The barcoded pyrosequencing analysis showed the similar bacterial community structures in intestines of BT and PW shrimp under a normal condition. However, during the time course exposure to V. harveyi, the relative abundance of bacteria belong to Vibrio genus was higher in the BT intestines at 12h after the exposure, whereas relative abundance of vibrios was more stable in PW intestines. The principle coordinates analysis based on weighted-UniFrac analysis showed that intestinal bacterial population in the BT shrimp lost their ability to restore their bacterial balance during the 72-h period of exposure to the pathogen, while the PW shrimp were able to reestablish their bacterial population to resemble those seen in the unexposed control group. This observation of bacterial disruption might correlate to different mortality rates observed between the two shrimp species

  19. Biology, genome organization and evolution of parvoviruses in marine shrimp

    Science.gov (United States)

    A number of parvoviruses are now know to infect marine shrimp, and these viruses alone or in combination with other viruses have the potential to cause major losses in shrimp aquaculture globally. This review provides a comprehensive overview of the biology, genome organization, gene expression, and...

  20. COMPARATIVE STUDIES ON NITROGEN BUDGETS OF CLOSED SHRIMP POLYCULTURE SYSTEMS"

    Institute of Scientific and Technical Information of China (English)

    齐振雄; 李德尚; 张曼平; 董双林

    2001-01-01

    April to October, 1997 comparative studios on the nitrogen budgets of closed shrimp polyculture systems showed that, in all the studied polyculture systems, nitrogen from feeds and fertilizers were the main input items, which comprised 70.7% - 83.9% of the total input nitrogen, 3.2% - 7.4% of which was provided by nitrogen fixation. It was in monoculture enclosures (Y-4, Y-11 and Y-12) that the percentage reached the maximum value. The output nitrogen in harvested products comprised 10.8% - 24.6% of total input nitrogen, and the highest percentage, 24.6%, was found in shrimp-fish-tagelus polyculture systems. In shrimp monoculture and shrimp-fish polyculture systems, they were 19.1% and 21.9%, respectively. The nitrogen utilization efficiency was different and varied from 12.2% to 20.1%. The highest, 20.1%, was found in shrimp-fish-tagelus polycultttre systems, and the average of 20.0% was found in shrimp-tagelus polyculture systems. The lowest, 12.2%, was found in shrimp monoculture systems.All the nitrogen utilization efficiencies in shrimp-fish systems or shrimp-scallop systems seemed to be higher than that of the monoculture system, but they showed little statistical difference. The main outputs of nitrngen were found in sediment mud, and comprised 48.2% -60.8% of the total input, the lowest percentage was found in shrimp-fish-tagelus polyculture systems, and the highost percentage in shrimp-scallop systems.During the experiment, nitrogen lost through denitrification and ammonia volatilization comprised 1.9% - 6.2%, averaged 2.8%, of the total input, and the loss through seepage comprised 5.9% - 8.9% of the total.The estimated nitrogen attached to the enclosure wall comprised 3.7% - 13.3% of the total, and was highest in shrimp monoculture systems. Compared with the classic shrimp farming industry, the closed shrimp polycul-ture systems may improve the nitrogen utilization efficiency, and hence reduce the environmental impacts on coastal waters. The nitrogen

  1. COMPARATIVE STUDIES ON NITROGEN BUDGETS OF CLOSED SHRIMP POLYCULTURE SYSTEMS

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    April to October, 1997 comparative studies on the nitrogen budgets of closed shrimp polyculture systems showed that, in all the studied polyculture systems, nitrogen from feeds and fertilizers were the main input items, which comprised 70.7%-83.9% of the total input nitrogen, 3.2%-7.4% of which was provided by nitrogen fixation. It was in monoculture enclosures (Y-4, Y-11 and Y-12) that the percentage reached the maximum value. The output nitrogen in harvested products comprised 10.8%-24.6% of total input nitrogen, and the highest percentage, 24.6%, was found in shrimp-fish-tagelus polyculture systems. In shrimp monoculture and shrimp-fish polyculture systems, they were 19.1% and 21.9%, respectively. The nitrogen utilization efficiency was different and varied from 12.2% to 20.1%. The highest, 20.1%, was found in shrimp-fish-tagelus polyculture systems, and the average of 20.0% was found in shrimp-tagelus polyculture systems. The lowest, 12.2%, was found in shrimp monoculture systems. All the nitrogen utilization efficiencies in shrimp-fish systems or shrimp-scallop systems seemed to be higher than that of the monoculture system, but they showed little statistical difference. The main outputs of nitrogen were found in sediment mud, and comprised 48.2%-60.8% of the total input, the lowest percentage was found in shrimp-fish-tagelus polyculture systems, and the highest percentage in shrimp-scallop systems. During the experiment, nitrogen lost through denitrification and ammonia volatilization comprised 1.9%-6.2%, averaged 2.8%, of the total input, and the loss through seepage comprised 5.9%-8.9% of the total. The estimated nitrogen attached to the enclosure wall comprised 3.7%-13.3% of the total, and was highest in shrimp monoculture systems. Compared with the classic shrimp farming industry, the closed shrimp polyculture systems may improve the nitrogen utilization efficiency, and hence reduce the environmental impacts on coastal waters. The nitrogen discharging

  2. Shelf life extension of dried shrimps by irradiation

    International Nuclear Information System (INIS)

    By reducing the number of bacteria, irradiation with a dose of 3 kGy increased the shelf life of dried and salted shrimp. Combining radiation with low-temperature storage further increased shelf life. Vacuum packaging the shrimp in polyethylene-laminated polyethylene bags resulted in extension of the shelf life by more than 15 weeks. Packing in tupperware and polyethylene bags was less effective. Most of the bacteria in the dried and salted shrimps were halophiles. Salmonellae and coliforms were not detected in the samples studied. During storage, ammonia was formed in the shrimps. Irradiation caused less ammonia to be formed. Low-temperature storage also reduced ammonia formation. The sensory quality of the shrimps decreased with increasing amounts of ammonia. (author). 9 refs, 25 figs

  3. Marine shrimp aquaculture and natural resource degradation in Thailand

    Science.gov (United States)

    Flaherty, Mark; Karnjanakesorn, Choomjet

    1995-01-01

    Rising demand for shrimp in the developed nations has helped to foster a dramatic growth in marine shrimp aquaculture, particularly in South America and South Asia. In Thailand, Marine shrimp aquaculture is now an important earmer of foreign exchange. The growth in Production has been achieved through the expansion of the culture area and the adoption of intensive production methods. The conversion of near-shore areas to shrimp culture, however, is proving to have many consequences that impinge on the environmental integrity of coastal areas. This paper reviews the development of Thailand's marine shrimp culture industry and examines the nature of the environmental impacts that are emerging. It then discusses the implications these have for rural poor and the long-term viability of the culture industry.

  4. Consumer method to control Salmonella and Listeria species in shrimp.

    Science.gov (United States)

    Edwards, Genevieve; Janes, Marlene; Lampila, Lucina; Supan, John

    2013-01-01

    The purpose of this study was to determine whether the current consumer method of boiling shrimp until floating and pink in color is adequate for destroying Listeria and Salmonella. Shrimp samples were submerged in bacterial suspensions of Listeria and Salmonella for 30 min and allowed to air dry for 1 h under a biosafety cabinet. Color parameters were then measured with a spectrophotometer programmed with the CIELAB system. Twenty-four shrimp samples were divided into groups (days 0, 1, or 2) and stored at 4°C. The samples were treated by placing them in boiling water (100°C) on days 0, 1, and 2. The shrimp were immediately removed from the boiling water once they floated to the surface, and color parameters were measured. Bacterial counts were determined, and the log CFU per gram was calculated. The effect of sodium tripolyphosphate on the color change of cooked shrimp also was determined. Initial bacterial counts on shrimp after air drying were 5.31 ± 0.14 log CFU/g for Salmonella Enteritidis, 5.24 ± 0.31 log CFU/g for Salmonella Infantis, 5.40 ± 0.16 log CFU/g for Salmonella Typhimurium, 3.91 + 0.11 log CFU/g for Listeria innocua, 4.45 ± 0.11 log CFU/g for Listeria monocytogenes (1/2a), and 3.70 ± 0.22 log CFU/g for Listeria welshimeri. On days 0, 1, and 2, all bacterial counts were reduced to nondetectable levels for shrimp samples that floated. The average time for shrimp to float was 96 ± 8 s. The bacterial counts remained at nondetectable levels (<10 log CFU/g) during refrigerated (4°C) storage of cooked shrimp for 2 days. The redness, yellowness, and lightness were significantly higher (P < 0.0001) for the cooked shrimp than for the uncooked shrimp on all days tested. The standard deviation for redness in the cooked shrimp was large, indicating a wide range of pink coloration on all days tested. The results suggest that boiling shrimp until they float will significantly reduce Listeria and Salmonella contamination, but color change is not a good

  5. Shrimp quality and safety management along the supply chain in Benin

    OpenAIRE

    Dabade, D.S.

    2015-01-01

    Abstract This thesis focuses on quality and safety management of tropical shrimp (Penaeus spp.) using Benin (West Africa) as an example of a shrimp exporting country. The entire supply chain, from fishing areas (brackish waters) to shrimp processing plants, was investigated. The steps of the chain prior to shrimp processing at the freezer plants were critical for shrimp quality and safety because of prevailing temperature abuse and inappropriate hygienic conditions. Combining culture-dependen...

  6. Impacts of Shrimp Farming on the Socioeconomic and Environmental Conditions in the Coastal Regions of Bangladesh

    OpenAIRE

    Islam, M S; M. Serajul Islam; Wahab, M.A.; A.A. Miah; A.H.M. Mustafa Kamal

    2003-01-01

    The study was carried out to analyze the comparative economic returns of alternate shrimp-crop farming and to assess the socioeconomic and environmental impacts of shrimp farming in coastal areas of Bangladesh. Shrimp farmers as well as other groups of people such as land lessors, shrimp farm labourers and shrimp seed collectors directly benefited and affected were randomly selected for the study. Accordingly, an appropriate number of all these sample households were selected from four differ...

  7. Beyond symbiosis: cleaner shrimp clean up in culture.

    Science.gov (United States)

    Militz, Thane A; Hutson, Kate S

    2015-01-01

    Cleaner organisms exhibit a remarkable natural behaviour where they consume ectoparasites attached to "client" organisms. While this behaviour can be utilized as a natural method of parasitic disease control (or biocontrol), it is not known whether cleaner organisms can also limit reinfection from parasite eggs and larvae within the environment. Here we show that cleaner shrimp, Lysmata amboinensis, consume eggs and larvae of a harmful monogenean parasite, Neobenedenia sp., in aquaculture. Shrimp consumed parasite eggs under diurnal (63%) and nocturnal (14%) conditions as well as infectious larvae (oncomiracidia) diurnally (26%). Furthermore, we trialled the inclusion of cleaner shrimp for preventative parasite management of ornamental fish, Pseudanthias squamipinnis, and found shrimp reduced oncomiracidia infection success of host fish by half compared to controls (held without shrimp). Fish held without cleaner shrimp exhibited pigmentation changes as a result of infection, possibly indicative of a stress response. These results provide the first empirical evidence that cleaner organisms reduce parasite loads in the environment through non-symbiotic cleaning activities. Our research findings have relevance to aquaculture and the marine ornamental trade, where cleaner shrimp could be applied for prophylaxis and control of ectoparasite infections. PMID:25706952

  8. Prediction of shelled shrimp weight by machine vision

    Institute of Scientific and Technical Information of China (English)

    Peng-min PAN; Jian-ping LI; Gu-lai LV; Hui YANG; Song-ming ZHU; Jian-zhong LOU

    2009-01-01

    The weight of shelled shrimp is an important parameter for grading process. The weight prediction of shelled shrimp by contour area is not accurate enough because of the ignorance of the shrimp thickness. In this paper, a multivariate prediction model containing area, perimeter, length, and width was established. A new calibration algorithm for extracting length of shelled shrimp was proposed, which contains binary image thinning, branch recognition and elimination, and length reconstruction, while its width was calculated during the process of length extracting. The model was further validated with another set of images from 30 shelled shrimps. For a comparison purpose, artificial neural network (ANN) was used for the shrimp weight predication. The ANN model resulted in a better prediction accuracy (with the average relative error at 2.67%), but took a tenfold increase in calculation time compared with the weight-area-perimeter (WAP) model (with the average relative error at 3.02%). We thus conclude that the WAP model is a better method for the prediction of the weight of shelled red shrimp.

  9. Determination of Contamination Profiles of Human Bacterial Pathogens in Shrimp Obtained from Java, Indonesia

    International Nuclear Information System (INIS)

    Shrimp continues to be an important export commodity for Indonesia and contributed significantly to the country’s revenue. However, shrimp exports have been frequently rejected by importing countries due to filth, Salmonella and insanitary conditions. This study was conducted to evaluate the profiles of bacterial contamination of ocean and aquaculture shrimp obtained from the area of West, Central and East Java; frozen shrimp and shrimp during industry production of frozen shrimp. The study indicated that both ocean and aquaculture shrimp obtained from the study area were heavily contaminated. On the average, shrimp obtained from West Java were more contaminated than those obtained from East and Central Java. The total bacterial counts were generally higher in ocean shrimp than those of aquaculture ones. Salmonella was present in two of 32 samples of ocean shrimp and in four of 32 samples of aquaculture shrimp obtained from the study area. Vibrio cholerae was not detected in shrimp from West Java, but was found in three out of 16 samples obtained from East and Central Java. V. parahaemolyticus was frequently identified in aquaculture shrimp but absent in fresh ocean shrimp. Studies on shrimp collected from six sampling points during frozen shrimp production revealed that processing will reduce the number of total bacterial, E. coli, and Staphylococal counts. However, the processing did not effectively reduce the incidence of Salmonella or V. parahaemolyticus when the raw material has been contaminated with the pathogens. Sizing and grading as well as arrangement of shrimp before freezing were considered as the critical points where bacteria should be controlled to inhibit growth and cross contamination with bacteria such as Listeria. Implementation of Good Agricultural Practices in production of raw shrimp as well as Hazard Analysis Critical Control Point at the line processing are expected to improve the quality of fresh and frozen shrimp. (author)

  10. Development of shrimp in small ponds

    Directory of Open Access Journals (Sweden)

    Armando Adolfo Ortega Salas

    2014-06-01

    Full Text Available Development of white shrimp Litopenaeus vannamei in small ponds ( 6 m3 in fresh water (2-3‰ and seawater; ponds 3.66 x 1.65 x 1.0 m; availability of fresh water, sea water, aeration and drainage. Two cycles of three months each were made. The postlarvae were acclimated to seawater fresh water in four days. Four hundred postlarvas/m3 were seeded in freshwater pond and 500 in the pool of seawater. First, a culture of Daphnia magna in the freshwater pond, also appeared chyronomid larvae; Artemia cysts were seeded in sea water as a dietary supplement. The shrimp were fed Camaronina (25% protein at libitum, daily; is offered on a tray of food; the temperature ranged between 27 and 30° C, oxygen 4.26 ± 1.43 mg / L , pH between 7 and 8 . Detritus siphoned every third day. Water changes between 10 and 20% are often performed. The feed conversion rate (FCR was 1:1.3 . The shrimp were measured in length and weight to calculate weekly growth by Bertalanffy model. Survival in the first cycle was 95.8 , and 97.9% for the second cycle. In seawater parameters of the population of the first cycle were k = 0.0301, L ∞ = 322.16 and t0 = -0.8852, the second cycle of k = 0.0203, L ∞ = 294.42 and t0 = -5.3771. The biomass of 27 kg was obtained for the first cycle and 16 kg for the second cycle. Freshwater population parameters of the first cycle were k = 0.0957, L ∞ = 146.98 and t0 = - 0.93; in the second cycle of k = 0.0172 , L ∞ = 367.82 and t0 = - 4.60. The biomass of 26 kg was obtained for the first cycle and 16 kg for the second cycle. The results indicate a rapid growth during the first 10 weeks. In small ponds can be handled well aseptic conditions without disease problems, good crop was obtained.

  11. COMPARISON OF CARBON AND NITROGEN FLUXES IN TIDEFLAT FOOD WEBS DOMINATED BY BURROWING SHRIMP OR BY CULTURED OYSTERS

    Science.gov (United States)

    Two species of indigenous, thalassinid burrowing shrimps are pests to the benthic culture of Pacific oysters (Crassostrea gigas) because deposition of sediment excavated by the shrimps buries or smothers the oysters. Carbaryl pesticide is used to reduce burrowing shrimp densitie...

  12. Shrimp farming in estuarine environment: Points to ponder

    Digital Repository Service at National Institute of Oceanography (India)

    Achuthankutty, C.T.

    The capture fishery resources have almost reached a sustainable yield and the production can only be supplemented through culture fishery practices. This is particularly true in case of marine shrimps. The annual foreign exchange earning from marine...

  13. 2008 Economic Survey of Gulf State Shrimp License Holders

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This mail survey collected data on the economic performance of active commercial shrimp harvesters who primarily operated in inshore waters of western Florida,...

  14. Sustainable shrimp farming in India - Prospects and challenges

    Digital Repository Service at National Institute of Oceanography (India)

    Achuthankutty, C.T.

    Shrimp farming has grown into a multi-crore industry in India. It has vast potential for further expansion. However, this growing industry needs to develop appropriate indigenous technologies in specific areas to make it a sustainable and profitable...

  15. DISSOLVED AIR FLOTATION TREATMENT OF GULF SHRIMP CANNERY WASTEWATER

    Science.gov (United States)

    This study reports on the operation of a plant scale dissolved air flotation system installed to define and evaluate attainable shrimp cannery wastewater treatment levels. The system was operated in all three modes of DAF pressurization. Destabilizing coagulants investigation inc...

  16. Toxic factors of Vibrio strains pathogenic to shrimp

    OpenAIRE

    Goarant, Cyrille; Herlin, Jose; Brizard, Raphael; Marteau, Anne-laure; Martin, C.; Martin, B.(CERN, Geneva, Switzerland)

    2000-01-01

    Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V: penaeicida, V. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish c...

  17. Production and characterization of Chitosan from shrimp shells waste

    OpenAIRE

    Anshar Patria

    2013-01-01

    This research aims to study the production of chitosan from shrimp shell waste origin andcharacterize the chitosan quality of shrimp shell waste that includes parameters yield, solubility,intrinsic viscosity, molecular weight and deacetylation degree. Results showed that the treatment ofheating temperature and heating time in the process of deacetylation caused significant (P≤0.01)influence on yield, solubility, intrinsic viscosity, molecular weight and deacetylation degree of producedchitosa...

  18. Bacterial Selection from Shrimp Ponds for Degradation of Organic Matters

    Directory of Open Access Journals (Sweden)

    Powtongsook, S.

    2007-01-01

    Full Text Available Accumulation of ammonia, nitrite and hydrogen sulfide in a shrimp pond is generally caused by incomplete degradation of residual organic matters from overfeeding and from organic wastes released by shrimps. The phenomenon affects shrimp growth and survival rate. The objectives of this investigation were to screen for a bacterial strain able to digest organic residues and to evaluate the changes of residues by bacterial activities under natural conditions. The results from this work showed that the isolated strain, Bacillus cereus S1, had the highest protease activity (57.1 U/ml with the presence of glucoamylase and lipase activities (4.5 and 0.13 U/ml, respectively. Under an aseptic condition in 1-L flasks containing seawater with 0.1% shrimp feed, B. cereus S1 degraded organic matters and significantly reduced chemical oxygen demand (COD (70.8%. An amount of ammonia-nitrogen was increased during the first 5 days of incubation due to the degradation of organic compounds in shrimp feed. However, it declined afterward with nitrate-nitrogen increase and unchanged nitrite nitrogen content. Under natural conditions in 10-L glass jars containing seawater with 0.05% shrimp feed and 0.05% sediment, B. cereus S1 and a commercial bacterial product (Inpicin-G could reduce COD (4.5% and 15.8%, respectively and biochemical oxygen demand (BOD (35.1 and 11.4%, respectively. However, similar changes of ammonia-nitrogen, nitrate-nitrogen and nitrite-nitrogen contents in water samples were observed. The results indicate that this selected bacterium could reduce organic compound accumulations on a laboratory scale. In addition, the strain did not produce any enterotoxins compared to a toxin standard. Therefore, the bacterium, Bacillus cereus S1, could be applied to decrease organic matters accumulated in shrimp pond without any harm to shrimps or consumers.

  19. Scenarios for Resilient Shrimp Aquaculture in Tropical Coastal Areas

    OpenAIRE

    Boer, Willem F; Roel Bosma; Han van Dijk; Leontine Visser; Van Zwieten, Paul A. M.; Simon R. Bush; Marc Verdegem

    2010-01-01

    We contend there are currently two competing scenarios for the sustainable development of shrimp aquaculture in coastal areas of Southeast Asia. First, a landscape approach, where farming techniques for small-scale producers are integrated into intertidal areas in a way that the ecological functions of mangroves are maintained and shrimp farming diseases are controlled. Second, a closed system approach, where problems of disease and effluent are eliminated in closed recirculation ponds behind...

  20. Brine Shrimp Ecology In The Great Salt Lake, Utah

    OpenAIRE

    Wurtsbaugh, Wayne A.

    1995-01-01

    Hypersaline lakes are noted for their simple communities which facilitate understanding ecological interactions (Williams et al. 1990; Wurtsbaugh 1992; Jellison and Melack 1988). Nevertheless, we still cannot easily predict how environmental changes will effect the population dynamics in these lakes, at least in part because even these simple ecosystems may be more complex than we .realize. Many hypersaline lakes are dominated by the brine shrimp Artemia spp. The production of brine shrimp is...

  1. Import price analysis of shrimp products in international market%虾产品国际市场进口价格分析

    Institute of Scientific and Technical Information of China (English)

    宁夏; 孙琛

    2012-01-01

    [Objective]Import and export status of shrimp products and its influencing factors were analyzed in order to provide references for government and corporate decision-making on shrimp product productions and sales. [Method] Through analyzing the supply and demand of major importing countries and their competitors regarding production factors that are affecting international shrimp import price, improvement measures and suggestions for the development of Chinese shrimp industry were put forward. [Result]Import price of shrimp products in the United States and Japan, despite being impacted by trade barrier, financial crisis, human errors, and natural disasters, slowly went through the normal business cycle with peaks and dips; on the other hand, the import price of shrimp products in Spain gradually descended with little fluctuation due to its significantly horrendous economic crisis. Regarding shrimp exports to different countries, China, Thailand, Indonesia, and India were the predominant producers and shrimp exports increased year by year. [Suggestion] In order to strengthen positive and stable growth of the Chinese shrimp trade, quality and quantity of shrimp products should be improved; export structure should be adjusted and industry organization should be promoted.%[目的]分析虾产品的贸易情况及影响因素,为我国政府及企业制定决策提供参考.[方法]通过对主要进口国的需求状况和生产国的竞争状况进行分析,探讨影响国际虾产品进口价格变动因素,并为我国虾产业的发展提供对策建议.[结果]美国和日本的虾产品进口价格呈缓慢下降至一定区域后再缓慢上升的趋势,影响因素包括贸易壁垒、金融危机及各种人为或自然灾害;西班牙则是呈缓慢下降至一定区域后呈小范围波动的趋势,受金融危机的影响较大.从各国的虾类产量来看,中国、泰国、印度尼西亚与印度是主要生产国,且产量呈逐年增加的趋势.[

  2. STRATEGIES FOR DEVELOPING SUSTAINABLE AND COMPETITIVE CLUSTER FOR SHRIMP INDUSTRY

    Directory of Open Access Journals (Sweden)

    Anas M. Fauzi

    2012-09-01

    Full Text Available Kampung Vannamei as shrimp cluster is being developed since 2004 by PT CP Prima, tbk Surabaya through Shrimp Culture Health Management transformation technology to several traditional farmers in Gresik, Lamongan, Tuban, and Madura areas. The research objectives aims to identify and mapping of stakeholder, to analyze interaction of stakeholders, to formulate strategy from internal and external environment factors and to set priority on strategy to develop sustainable and competitive shrimp cluster in the Kampung vannamei. Primary data was collected through stakeholders’ discussion forums, questionnaires, and interviews with relevant actors. Observations to the business unit also performed to determine the production and business conditions, particularly in capturing information about the threat and challenges. While the secondary data is used in policy documents national and local area statistics, and relevant literature. Analyses were performed by using the SRI International cluster pyramid, diamond porter’s analysis, SWOT and Matrix TOWS analysis, and analytical hierarchy process. Analyses were performed by the methods discussed in qualitative and descriptive. There are 7 strategies could be implemented to develop sustainable and competitive shrimp cluster. However, it is recommended to implement the strategy base on priority, which the first priority is strategy to improve linkages between businesses in the upstream and downstream industries into multi stakeholders’ platform in shrimp industry.Keywords: Shrimp, Cluster, Competitiveness, Diamond Porter, SWOT Analysis, AHP

  3. Protection of blue shrimp (Litopenaeus stylirostris) against the White Spot Syndrome Virus (WSSV) when injected with shrimp lysozyme.

    Science.gov (United States)

    Mai, Wei-jun; Wang, Wei-na

    2010-04-01

    In this study we found that a blue shrimp (Litopenaeus stylirostris) lysozyme gene (Lslzm) was up-regulated in WSSV-infected shrimp, suggesting that lysozyme is involved in the innate response of shrimp to this virus. Shrimp were intramuscularly injected with Lslzm protein to identify how this recombinant protein protects L. stylirostris from WSSV infection and to determine how this protein influences nonspecific cellular and humoral defense mechanisms. Higher survival rates and a lower viral load (compared with controls) were reported for shrimps that were first injected with the Lslzm protein and then infected with WSSV. In addition, the Lslzm expression level and the immunological parameters (including THC, phagocytic activity, respiratory burst activity, phenoloxidase activity and lysozyme activity) were all significantly higher in the WSSV-infected shrimp treated with the Lslzm protein, compared with the controls. These results indicate that lysozyme is effective at blocking WSSV infection in L. stylirostris and that lysozyme modulates the cellular and humoral defense mechanisms after they are suppressed by the WSSV virus. PMID:20074645

  4. Chinese Cooking.

    Science.gov (United States)

    Kane, Tony

    This unit, intended for secondary level students, is a general introduction to Chinese cooking. It is meant to inform students about the origins of Chinese cooking styles in their various regional manifestations, and it can be used to discuss how and why different cultures develop different styles of cooking. The first part of the unit, adapted…

  5. Economic Data Collection for Gulf of Mexico South Atlantic Shrimp Fisheries

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Annual Economic Survey of Federal Gulf and Atlantic Shrimp Permit Holders collects data about operating expenses and costs of owning and maintaining shrimp...

  6. 75 FR 12175 - Certain Frozen Warmwater Shrimp from India: Preliminary Results of Antidumping Duty...

    Science.gov (United States)

    2010-03-15

    .... sales: sales planning, personnel training, sales promotion, packing, inventory maintenance in India... performed the following selling functions: sales planning, personnel training, sales promotion, packing... Antidumping Duty Order: Certain Frozen Warmwater Shrimp from India, 70 FR 5147 (Feb. 1, 2005) (Shrimp...

  7. Simulating environmental effects on brown shrimp production in the northern Gulf of Mexico

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Brown shrimp (Farfantepenaeus aztecus) are a commercially important fishery species of the northern Gulf of Mexico. Young shrimp settle in estuarine salt marsh...

  8. Conflicting interests in the use of Kerala's penaeid shrimp resources: A case question

    Digital Repository Service at National Institute of Oceanography (India)

    Stephen, D.; David, J.; Anand, P.E.V.

    In the drive to increase foreign exchange on the shrimp resource base, the development strategy has been to bring additional backwater area under the open semi-intensive culture system. Apparently, the nature of the shrimp resource complex...

  9. 78 FR 33345 - Certain Frozen Warmwater Shrimp From Malaysia: Preliminary Countervailing Duty Determination

    Science.gov (United States)

    2013-06-04

    ... International Trade Administration Certain Frozen Warmwater Shrimp From Malaysia: Preliminary Countervailing... shrimp) from Malaysia. The period of investigation (POI) is January 1, 2011, through December 31, 2011... Malaysia,'' dated concurrently with this notice (Preliminary Decision Memorandum). For this...

  10. Rice versus Shrimp Production in Thailand: Is There Really a Conflict?

    OpenAIRE

    Mekhora, Thamrong; McCann, Laura M.J.

    2003-01-01

    Shrimp farming in Thailand has had disastrous effects on the environment in the past, which has prompted a government ban on shrimp production in inland areas. However, a new low-salinity shrimp farming system has developed that seems to have fewer disease and environmental problems than previous systems but competes with rice production for land and water resources. The present study found that shrimp farming exhibits increasing returns to scale and is much more profitable than rice farming,...

  11. Comparative Study of Traditional Vs. Scientific Shrimp Farming in West Bengal: A Technical Efficiency Analysis

    OpenAIRE

    Poulomi Bhattacharya

    2008-01-01

    Applying a Stochastic Production Frontier to farm-level data from shrimp farmers in West Bengal, India, this paper examines technical efficiency and its determinants in both scientific and traditional shrimp farming systems. The empirical results suggest high degrees of technical inefficiency among the shrimp farmers at household level. The scientific shrimp farmers have a higher technical efficiency than their traditional counterparts. This necessitates government policy initiatives and exte...

  12. Decomposing mangrove litter supports a microbial biofilm with potential nutritive value to penaeid shrimp post larvae

    OpenAIRE

    Gatune, C.; Vanreusel, A.; Cnudde, C.; Ruwa, R.; Bossier, P; M. De Troch

    2012-01-01

    The use of fish meal in shrimp culture not only contributes to the decline of wild fish stocks, but also undermines its profitability and enhances ecosystem pollution. There is an urgent need for alternative natural food supply in shrimp cultures. The present study investigated the potential of mangrove litter from Rhizophora mucronata and the associated microbial biofilm as food for shrimp post larvae of Penaeus indicus and Penaeus monodon in a community-based ecological shrimp farm in Mtwap...

  13. Global and local governance of shrimp farming in the Mekong Delta, Vietnam

    OpenAIRE

    Tran Thi Thu, H.

    2012-01-01

    The Mekong Delta is one of seven ecological regions in Vietnam where aquaculture and shrimp products are internationally traded and the shrimp farmers are firmly embedded in a global system of production and trade. The growth of shrimp aquaculture, in addition to population growth and higher levels of investment, has left coastal resources in the Mekong Delta increasingly vulnerable to rapid changes in land and resource use. The shrimp industry, made up of multiple stakeholders and fragmented...

  14. Quality perceptions of stakeholders in Beninese export-oriented shrimp chain.

    Science.gov (United States)

    Dabade, D Sylvain; den Besten, Heidy M W; Azokpota, Paulin; Nout, M J Rob; Hounhouigan, D Joseph; Zwietering, Marcel H

    2014-09-01

    In recent years, the Beninese shrimp sector has faced a ban on export to the European Union due to lack of compliance with food safety standards. The present study aimed at obtaining insight into the factors that determine shrimp quality and safety in Benin. A survey was conducted to investigate the relationships between stakeholders, the conditions under which shrimps are handled at fishing areas and processed at shrimp plants, and the stakeholders' perceptions of quality. A total of 325 fishermen, 128 intermediate traders, 12 collectors, and 3 shrimp processing plant managers were interviewed face to face. The results showed that various specific relations existed between the stakeholders. For example, loyalty was ensured by family relationships, or incentives were provided to ensure a supply of shrimps between stakeholders. Shrimp handling practices during the steps prior to shrimp processing at the plants were not in agreement with the requirements of the European regulations. For example, shrimps were kept at ambient temperature (28 ± 1°C) by 94.1% of fishermen and 60.9% of intermediate traders. Shrimps were also stored in inappropriate holding containers and washed with nonpotable water. Fishermen, intermediate traders, and collectors considered shrimp size and texture their priority quality attributes, whereas plant managers considered shrimp appearance (freshness) and texture their priority quality attributes. This survey demonstrated that the steps prior to shrimp processing at the plants are the critical steps for shrimp quality and safety because of temperature abuse and inappropriate hygienic conditions. There is a need to communicate and provide incentives for the stakeholders in the first part of the chain to give priority to shrimp freshness. Moreover, training in Good Fishing Practices and safe food handling practices and evaluation of compliance with the practices through monitoring will contribute to better shrimp quality and safety management

  15. 77 FR 36998 - Proposed Information Collection; Comment Request; Mandatory Shrimp Vessel and Gear...

    Science.gov (United States)

    2012-06-20

    ... Shrimp Vessel and Gear Characterization Survey AGENCY: National Oceanic and Atmospheric Administration... Shrimp Fishery Management Plan (FMP). The regulations for the Gulf Shrimp Vessel and Gear... and Gear Characterization Form. The form will be provided by NMFS at the time of permit...

  16. 78 FR 40436 - Proposed Information Collection; Comment Request; Mandatory Shrimp Vessel and Gear...

    Science.gov (United States)

    2013-07-05

    ... Shrimp Vessel and Gear Characterization Survey AGENCY: National Oceanic and Atmospheric Administration... Shrimp Fishery Management Plan (FMP). The regulations for the Gulf Shrimp Vessel and Gear... Gear Characterization Form. The form will be provided by NMFS at the time of permit application...

  17. 75 FR 22370 - Certain Frozen Warmwater Shrimp From Brazil, Ecuador, India, the People's Republic of China...

    Science.gov (United States)

    2010-04-28

    ... Value: Certain Frozen and Canned Warmwater Shrimp From Brazil, 69 FR 76910 (December 23, 2004) (Brazil...: Certain Frozen and Canned Warmwater Shrimp from the People's Republic of China, 69 FR 70997 (December 8... Than Fair Value and Antidumping Duty Order: Certain Frozen Warmwater Shrimp from Brazil, 70 FR...

  18. Shrimp disease management using bioactive marine secondary metabolites: an eco-friendly approach

    OpenAIRE

    Selvin, J.; Lipton, A.P.

    2003-01-01

    Vibriosis caused by opportunistic and secondary bacterial pathogens is still a serious disease problem in aquaculture of the black tiger shrimp Penaeus monodon. Attempts were made for controlling shrimp bacterial disease using Marine Secondary Metabolites (MSMs). Findings indicated that the MSMs of seaweed Ulva fasciata and Dendrilla nigra are effective for controlling shrimp bacterial pathogens.

  19. Global and local governance of shrimp farming in the Mekong Delta, Vietnam

    NARCIS (Netherlands)

    Tran Thi Thu, H.

    2012-01-01

    The Mekong Delta is one of seven ecological regions in Vietnam where aquaculture and shrimp products are internationally traded and the shrimp farmers are firmly embedded in a global system of production and trade. The growth of shrimp aquaculture, in addition to population growth and higher levels

  20. 77 FR 31062 - Programs To Reduce Incidental Capture of Sea Turtles in Shrimp Fisheries; Certifications Pursuant...

    Science.gov (United States)

    2012-05-24

    ... governing the incidental capture of sea turtles in its commercial shrimp fishery comparable to the program... regulatory program in their commercial shrimp trawl fishery. The Department also certified 26 shrimp... are: the Bahamas, Belize, China, the Dominican Republic, Fiji, Hong Kong, Jamaica, Oman, Peru,...

  1. 78 FR 50379 - Certain Frozen Warmwater Shrimp From Thailand: Final Negative Countervailing Duty Determination

    Science.gov (United States)

    2013-08-19

    ..., Inc.; Hi-Seas of Dulac, Inc.; Indian Ridge Shrimp Co.; JBS Packing Co., Inc.; Lafitte Frozen Foods... Shrimp From Thailand: Preliminary Countervailing Duty Determination, 78 FR 33350 (June 4, 2013..., ``Certain Frozen Warmwater Shrimp from Ecuador, India, Indonesia, Malaysia, People's Republic of...

  2. 78 FR 50391 - Certain Frozen Warmwater Shrimp From the People's Republic of China: Final Affirmative...

    Science.gov (United States)

    2013-08-19

    ....; Indian Ridge Shrimp Co.; JBS Packing Co., Inc.; Lafitte Frozen Foods Corp.; M&M Shrimp (Biloxi Freezing... From the People's Republic of China: Preliminary Countervailing Duty Determination, 78 FR 33346 (June 4..., ``Certain Frozen Warmwater Shrimp from Ecuador, India, Indonesia, Malaysia, People's Republic of...

  3. Microbial changes and growth of Listeria monocytogenes during chilled storage of brined shrimp ( Pandalus borealis )

    DEFF Research Database (Denmark)

    Mejlholm, Ole; Kjeldgaard, J.; Modberg, A.;

    2008-01-01

    Thirteen storage trials and ten challenge tests were carried out to examine microbial changes, spoilage and the potential growth of Listeria monocytogenes in brined shrimp (Pandalus borealis). Shrimp in brine as well as brined and drained shrimp in modified atmosphere packaging (MAP) were produce...

  4. Toxic factors of Vibrio strains pathogenic to shrimp.

    Science.gov (United States)

    Goarant, C; Herlin, J; Brizard, R; Marteau, A L; Martin, C; Martin, B

    2000-03-14

    Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V. penaeicida, V. alginolyticus and V. nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus stylirostris) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida was found only after cultivation at low temperature (20 degrees C) and not at higher temperature (30 degrees C). This points to the fact that 'Syndrome 93' episodes are triggered by temperature drops. The assays used here demonstrate the usefulness of primary shrimp cell cultures to study virulence mechanisms of shrimp pathogenic bacteria. PMID:10782343

  5. Immunological-based assays for specific detection of shrimp viruses.

    Science.gov (United States)

    Chaivisuthangkura, Parin; Longyant, Siwaporn; Sithigorngul, Paisarn

    2014-02-12

    Among shrimp viral pathogens, white spot syndrome virus (WSSV) and yellow head virus (YHV) are the most lethal agents, causing serious problems for both the whiteleg shrimp, Penaeus (Litopenaeus) vannamei, and the black tiger shrimp, Penaeus (Penaeus) monodon. Another important virus that infects P. vannamei is infectious myonecrosis virus (IMNV), which induces the white discoloration of affected muscle. In the cases of taura syndrome virus and Penaeus stylirostris densovirus (PstDNV; formerly known as infectious hypodermal and hematopoietic necrosis virus), their impacts were greatly diminished after the introduction of tolerant stocks of P. vannamei. Less important viruses are Penaeus monodon densovirus (PmDNV; formerly called hepatopancreatic parvovirus), and Penaeus monodon nucleopolyhedrovirus (PemoNPV; previously called monodon baculovirus). For freshwater prawn, Macrobrachium rosenbergii nodavirus and extra small virus are considered important viral pathogens. Monoclonal antibodies (MAbs) specific to the shrimp viruses described above have been generated and used as an alternative tool in various immunoassays such as enzyme-linked immunosorbent assay, dot blotting, Western blotting and immunohistochemistry. Some of these MAbs were further developed into immunochromatographic strip tests for the detection of WSSV, YHV, IMNV and PemoNPV and into a dual strip test for the simultaneous detection of WSSV/YHV. The strip test has the advantages of speed, as the result can be obtained within 15 min, and simplicity, as laboratory equipment and specialized skills are not required. Therefore, strip tests can be used by shrimp farmers for the pond-side monitoring of viral infection. PMID:24567913

  6. Feed palatability and the alternative protein sources in shrimp feed

    Directory of Open Access Journals (Sweden)

    Chutima Tantikitti

    2014-02-01

    Full Text Available Feed palatability in carnivorous aquaculture species, shrimps in particular, has been crucially related to the presence of compounds acting as attractants that are commonly associated with the prey components under wild conditions. Thus a nutritionally adequate and organoleptically-pleasing diet is essential to achieve satisfactory intake and growth in shrimps. Historically, fishmeal has been an essential dietary component of intensive shrimp cultures because of its nutrient composition and compounds of high attractability. However, in recent years the fishmeal supplies have been dwindling due to over hunting, a diminishing natural fish-stock, elevating prices and market volatility. This has led to search for cheaper sources of suitable protein as fishmeal substitutes. To improve the palatability of diets, various substances have been investigated for their effectiveness in aqua-feed including natural feed ingredients and synthetic flavor substances. For crustacean, attractants characteristically are of low molecular weight, water and ethanol soluble, and amphoteric or basic compounds that are released from potential prey items. Compounds such as free amino acids, especially taurine, hydroxyproline, glycine, arginine, glutamic acid and alanine have been identified to stimulate feeding in shrimps. The same has been identified with organic acids, nucleotides and nucleosides, betaine, and some small peptides. Palatability also has been associated with animal’s past experience with the feed. Understanding the factors that regulate feed palatability is therefore primary for successful shrimp culture.

  7. Bioaccumulation of animal adenoviruses in the pink shrimp

    Directory of Open Access Journals (Sweden)

    Roger B. Luz

    2015-09-01

    Full Text Available Adenoviruses are among the most promising viral markers of fecal contamination. They are frequently found in the water, sediment and soil of regions impacted by human activity. Studies of the bioaccumulation of enteric viruses in shrimp are scarce. The cities located in the northern coast of the lake systems in Southern Brazil have high urbanization and intensive farming rates, and poor sewage collection and treatment. One hundred (n = 100 Farfantepenaeus paulensis pink-shrimp specimens and 48 water samples were collected from coastal lagoons between June 2012 and May 2013. Water samples were concentrated and the shrimp, mashed. After DNA extraction, samples were analyzed by real time polymerase chain reaction (qPCR in order to detect and quantify viral genomes. Thirty-five percent of shrimp samples were positive for contamination, predominantly by avian adenoviruses. A total of 91.7% of water samples contained adenoviruses DNA, with the human form being the most frequent. Our results provided evidence of significant bioaccumulation of adenoviruses in shrimp, showing the extent of the impact of fecal pollution on aquatic ecosystems.

  8. Bioaccumulation of animal adenoviruses in the pink shrimp.

    Science.gov (United States)

    Luz, Roger B; Staggemeier, Rodrigo; Fabres, Rafael B; Soliman, Mayra C; Souza, Fernanda G; Gonçalves, Raoni; Fausto, Ivone V; Rigotto, Caroline; Heinzelmann, Larissa S; Henzel, Andréia; Fleck, Juliane D; Spilki, Fernando R

    2015-01-01

    Adenoviruses are among the most promising viral markers of fecal contamination. They are frequently found in the water, sediment and soil of regions impacted by human activity. Studies of the bioaccumulation of enteric viruses in shrimp are scarce. The cities located in the northern coast of the lake systems in Southern Brazil have high urbanization and intensive farming rates, and poor sewage collection and treatment. One hundred (n = 100) Farfantepenaeus paulensis pink-shrimp specimens and 48 water samples were collected from coastal lagoons between June 2012 and May 2013. Water samples were concentrated and the shrimp, mashed. After DNA extraction, samples were analyzed by real time polymerase chain reaction (qPCR) in order to detect and quantify viral genomes. Thirty-five percent of shrimp samples were positive for contamination, predominantly by avian adenoviruses. A total of 91.7% of water samples contained adenoviruses DNA, with the human form being the most frequent. Our results provided evidence of significant bioaccumulation of adenoviruses in shrimp, showing the extent of the impact of fecal pollution on aquatic ecosystems. PMID:26413052

  9. Brine shrimp cytotoxic activities of Hippophae rhamnoides Linn leaves extracts

    Institute of Scientific and Technical Information of China (English)

    JavidAli; BashirAhmad

    2015-01-01

    Objective: To evaluate brine shrimp lethality assay of solvent extracts (aqueous, methanol, ethanol, acetone, ethyl acetate, chloroform and n-hexane) of Hippophae rhamnoides (H. rhamnoides) leaves. Methods: Brine shrimp cytotoxicity assay was used to assess the cytotoxic potential of H. rhamnoides leaves extracts. Three vials for concentration of each extract were made and 10 shrimps per vial (30 shrimps per dilution) were transferred to specific concentration of each extract. Results: The mortality of aqueous extract was 46.7%, methanol extract was 46.7%, ethanolic extract was 50.0%, ethyl acetate was 26.7%, acetone extract was 33.3%, chloroform extract was 40.0% and n-hexane extract was 33.3%. The lowest LD50 was found in methanol extracts (1199.97 µg/mL). Brine shrimp cytotoxicity of tested extracts of H. rhamnoides showed that mortality rate was concentration dependent. Conclusions: It is concluded that bioactive components are present in all leaves extracts of H. rhamnoides, which could be accounted for its pharmacological effects. Thus, the results support the uses of this plant species in traditional medicine.

  10. Shrimp cephalothorax meal in laying hen diets.

    Directory of Open Access Journals (Sweden)

    Catalina Salas-Durán

    2015-06-01

    Full Text Available The aim of this study was to meassure the effect of shrimp meal (SM in commercial laying hen diets. From April to September 2013, in Costa Rica, Pleuroncodes planipes was used to obtain a meal (SM with a yield of 15%, particle size of 256 μg and negative for Salmonella sp. Proximate analysis was performed to the SM: crude protein (40.67%, ether extract (11.05%, crude fiber (7.12%, ash (27.48%, calcium (9.03%, phosphorus (2.66%, amino acid profile, pepsin digestibility (84% and acidity (8.34. Subsequently, a trial was performed with 140 40-week-old Hy-Line Brown laying hens, fed with four different diets containing increasing levels of inclusion of SM (0%, 5%, 10%, and 15% during four weeks; and formulated according to the ideal protein and digestible amino acids concepts; being isocaloric and isoproteic. The variables experimentally evaluated were: production percentage, feed intake, body weight, mortality, egg weight and feed conversion ratio. Only egg weight changed significantly between treatments in the third week (p<0.05. The hens fed with 5% SM laid heavier eggs. It is suggested to evaluate a level of SM inclusion up to 15% in laying hens diets.

  11. Monogamy in a Hyper-Symbiotic Shrimp.

    Science.gov (United States)

    Baeza, J Antonio; Simpson, Lunden; Ambrosio, Louis J; Guéron, Rodrigo; Mora, Nathalia

    2016-01-01

    Theory predicts that monogamy is adaptive in resource-specialist symbiotic crustaceans inhabiting relatively small and morphologically simple hosts in tropical environments where predation risk away from hosts is high. We tested this prediction in Pontonia manningi, a hyper-symbiotic shrimp that dwells in the mantle cavity of the Atlantic winged oyster Pteria colymbus that, in turn, infects gorgonians from the genus Pseudopterogorgia in the Caribbean Sea. In agreement with theory, P. manningi were found dwelling as heterosexual pairs in oysters more frequently than expected by chance alone. Males and females also inhabited the same host individual independent of the female gravid condition or of the developmental stage of brooded embryos. While the observations above argue in favor of monogamy in P. manningi, there is evidence to suggest that males of the studied species are moderately promiscuous. That females found living solitary in oysters most often brooded embryos, and that males allocated more to weaponry (major claw size) than females at any given size suggest that males might be roaming among host individuals in search of and, fighting for, receptive females. All available information depicts a rather complex mating system in P. manningi: primarily monogamous but with moderately promiscuous males. PMID:26934109

  12. Chinese astronomy

    OpenAIRE

    Macfarlane, Alan; Cullen, Christopher

    2004-01-01

    Standing in the observatory in Beijing, Christopher Cullen discusses the nature and sophistication of Chinese astronomy in the medieval period. The political as well as the intellectual interest in astronomy is outlined.

  13. Chinese Confucianism

    OpenAIRE

    Macfarlane, Alan; Cullen, Christopher

    2004-01-01

    Confucianism has deeply influenced Chinese civilization. Christopher Cullen describes its effect on education, social structure and knowledge over the past centuries, against the backdrop of a Confucian building in Beijing.

  14. Microprobe analysis of brine shrimp grown on meteorite extracts

    International Nuclear Information System (INIS)

    Nuclear microprobe methods have been used to investigate the uptake and distribution of various elements by brine shrimps and their unhatched eggs when grown in extracts of the Murchison and Allende carbonaceous meteorites, which were selected as model space resources. Measurements were carried out using a focussed 2 MeV proton beam raster scanned over the samples in order to obtain the average elemental concentrations. Line scans across the egg and shrimp samples show uptake of elements such as Mg, Ni, S and P which are present in the meteorites. The results confirmed that carbonaceous chondrite materials can provide nutrients, including high levels of the essential nutrient phosphate. The concentrations of these elements varied significantly between shrimp and eggs grown in extracts of the two meteorite types, which can help in identifying optimal growth media. Our results illustrate that nuclear microprobe techniques can determine elemental concentrations in organisms exposed to meteorite derived media and thus help in identifying useful future resources

  15. Detection of irradiation in cuticles of commercial shrimps

    International Nuclear Information System (INIS)

    Laboratory for Detection of Irradiated Food has more and more orders for detection of irradiation in shrimps. A method adapted to achieve detection of irradiation in this material is a thermoluminescence measure. However, the procedure is time-consuming - needs several days to receive the result. The aim of presented study was to elaborate the EPR examination of the possible irradiation in this product. As a result od the performed studies it can be concluded, that the EPR measurement of cuticles of shrimps can be adapted as the preliminary, screening test proving the irradiation of shrimp. It has to be stressed, however, that the lack of a specific, hydroxyapatite born EPR signal in the spectrum cannot suggest that sample was not irradiated

  16. Microprobe analysis of brine shrimp grown on meteorite extracts

    Energy Technology Data Exchange (ETDEWEB)

    Kennedy, J. [National Isotope Centre, GNS Science, 30 Gracefield Road, Lower Hutt (New Zealand)]. E-mail: j.kennedy@gns.cri.nz; Mautner, M.N. [Soil, Plant and Ecological Sciences Division, Lincoln University (New Zealand) and Department of Chemistry, University of Canterbury, Christchurch 8001 (New Zealand)]. E-mail: m.mautner@solis1.com; Barry, B. [National Isotope Centre, GNS Science, 30 Gracefield Road, Lower Hutt (New Zealand); Markwitz, A. [National Isotope Centre, GNS Science, 30 Gracefield Road, Lower Hutt (New Zealand)

    2007-07-15

    Nuclear microprobe methods have been used to investigate the uptake and distribution of various elements by brine shrimps and their unhatched eggs when grown in extracts of the Murchison and Allende carbonaceous meteorites, which were selected as model space resources. Measurements were carried out using a focussed 2 MeV proton beam raster scanned over the samples in order to obtain the average elemental concentrations. Line scans across the egg and shrimp samples show uptake of elements such as Mg, Ni, S and P which are present in the meteorites. The results confirmed that carbonaceous chondrite materials can provide nutrients, including high levels of the essential nutrient phosphate. The concentrations of these elements varied significantly between shrimp and eggs grown in extracts of the two meteorite types, which can help in identifying optimal growth media. Our results illustrate that nuclear microprobe techniques can determine elemental concentrations in organisms exposed to meteorite derived media and thus help in identifying useful future resources.

  17. Microprobe analysis of brine shrimp grown on meteorite extracts

    Science.gov (United States)

    Kennedy, J.; Mautner, M. N.; Barry, B.; Markwitz, A.

    2007-07-01

    Nuclear microprobe methods have been used to investigate the uptake and distribution of various elements by brine shrimps and their unhatched eggs when grown in extracts of the Murchison and Allende carbonaceous meteorites, which were selected as model space resources. Measurements were carried out using a focussed 2 MeV proton beam raster scanned over the samples in order to obtain the average elemental concentrations. Line scans across the egg and shrimp samples show uptake of elements such as Mg, Ni, S and P which are present in the meteorites. The results confirmed that carbonaceous chondrite materials can provide nutrients, including high levels of the essential nutrient phosphate. The concentrations of these elements varied significantly between shrimp and eggs grown in extracts of the two meteorite types, which can help in identifying optimal growth media. Our results illustrate that nuclear microprobe techniques can determine elemental concentrations in organisms exposed to meteorite derived media and thus help in identifying useful future resources.

  18. The Effect of Latitudinal Variation on Shrimp Reproductive Strategies

    Science.gov (United States)

    van de Kerk, Madelon; Jones Littles, Chanda; Saucedo, Omar; Lorenzen, Kai

    2016-01-01

    Reproductive strategies comprise the timing and frequency of reproductive events and the number of offspring per reproductive event, depending on factors such as climate conditions. Therefore, species that exhibit plasticity in the allocation of reproductive effort can alter their behavior in response to climate change. Studying how the reproductive strategy of species varies along the latitudinal gradient can help us understand and predict how they will respond to climate change. We investigated the effects of the temporal allocation of reproductive effort on the population size of brown shrimp (Farfantepenaeus aztecus) along a latitudinal gradient. Multiple shrimp species exhibit variation in their reproductive strategies, and given the economic importance of brown shrimp to the commercial fishing sector of the Unites States, changes in the timing of their reproduction could have significant economic and social consequences. We used a stage-based, density-dependent matrix population model tailored to the life history of brown shrimp. Shrimp growth rates and environmental carrying capacity were varied based on the seasonal climate conditions at different latitudes, and we estimated the population size at equilibrium. The length of the growing season increased with decreasing latitude and the reproductive strategy leading to the highest population size changed from one annual birth pulse with high reproductive output to continuous low-output reproduction. Hence, our model confirms the classical paradigm of continuous reproduction at low latitudes, with increased seasonality of the breeding period towards the poles. Our results also demonstrate the potential for variation in climate to affect the optimal reproductive strategy for achieving maximum population sizes. Certainly, understanding these dynamics may inform more comprehensive management strategies for commercially important species like brown shrimp. PMID:27158895

  19. Identification of Effective Organic Carbon for Biofloc Shrimp Culture System

    Directory of Open Access Journals (Sweden)

    K. Sakkaravarthi

    2015-01-01

    Full Text Available This study aims to identify an effective carbon source which enhances not only the microbial load but also the shrimps growth. The carbon source cane sugar, molasses and jaggery were tested in 100 L culture tanks with stocking density of 30 m‾2 Peneus monodon seeds for a culture period of 40 days. Jaggery biofloc tank shows highest floc formation (22 mL, highest survival (86% and highest ABW (6 g, proximate composition of jagerry biofloc had higher protein level (35% followed by mollases and sugar culture tanks. It confirms jaggery is a new efficient carbon source for shrimp culture system.

  20. Issues, Impacts, and Implications of Shrimp Aquaculture in Thailand

    Science.gov (United States)

    Dierberg; Kiattisimkul

    1996-09-01

    Water quality impacts to and from intensive shrimp aquaculture in Thailand are substantial. Besides the surface and subsurface salinization of freshwaters, loadings of solids, oxygen-consuming organic matter, and nutrients to receiving waters are considerable when the cumulative impacts from water exchange during the growout cycle, pond drainage during harvesting, and illegal pond sediment disposal are taken into account. Although just beginning to be considered in Thailand, partial recirculating and integrated intensive farming systems are producing promising, if somewhat limited, results. By providing on-site treatment of the effluent from the shrimp growout ponds, there is less reliance on using outside water supplies, believed to be the source of the contamination.The explosion in the number of intensively operated shrimp farms has not only impacted the coastal zone of Thailand, but has also resulted in an unsustainable aquaculture industry. Abandonment of shrimp ponds due to either drastic, disease-caused collapses or more grandual, year-to-year reductions in the productivity of the pond is common. To move Thailand towards a more sustainable aquaculture industry and coastal zone environment, integrated aquaculture management is needed. Components of integrated aquaculture management are technical and institutional. The technical components involve deployment of wastewater treatment and minimal water-use systems aimed at making aquaculture operations more hydraulically closed. Before this is possible, technical and economic feasibility studies on enhanced nitrification systems and organic solids removal by oxidation between production cycles and/or the utilization of plastic pond liners need to be conducted. The integration of semi-intensive aquaculture within mangrove areas also should be investigated since mangrove losses attributable to shrimp aquaculture are estimated to be between 16 and 32 % of the total mangrove area destroyed betweeen 1979 and 1993

  1. [The use of shrimp and turkey buzzard flour and milk whey in food for shrimp under controlled laboratory conditions].

    Science.gov (United States)

    Arosemena, G

    1990-01-01

    Two diets were prepared for the shrimp's development in the tanks, based on fish meals, shrimp's head meals, "gallinaza" and milk whey. The diets had a protein concentration of 37.8% and 45%, using as control a commercial diet with 35% of protein. Twelve juvenile shrimps with average weights of 2.30 gr. to 3.09 gr were used in each of the six-40 liter aquariums under laboratory controlled conditions for a 28 days period. The growth increments were low in spite of the high protein concentration, due to a deficiency in some essential aminoacid and were not successful in supplementing it with the protein sources used. The aquarium areas turned out to be limiting and growth was affected by density. The statistic analysis showed no significant differences in the treatments used. PMID:2330427

  2. Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio.

    Science.gov (United States)

    Munoz, M; Vandenbulcke, F; Garnier, J; Gueguen, Y; Bulet, P; Saulnier, D; Bachère, E

    2004-04-01

    The present study reports for the first time the involvement of an antimicrobial peptide in the defense reactions of a shrimp infected by a pathogenic Vibrio, Vibrio penaeicida. New members of the penaeidin family were characterized in the shrimp Litopenaeus stylirostris by RT-PCR and RACE-PCR from hemocyte total RNAs, and by mass spectrometry detection and immunolocalization of mature peptides in shrimp hemocytes. In infected shrimps, bacteria and penaeidin distribution colocalized in the gills and the lymphoid organ that represented the main infected sites. Moreover, the shrimp immune response to infection involved massive hemocyte recruitment to infection sites where released penaeidin may participate in the isolation and elimination of the bacteria, We show that the ability of the shrimps to circumvent shrimp infections is closely related to a recovery phase based on the hematopoietic process. PMID:15095016

  3. A feasibility study of gamma irradiation on Thailand frozen shrimps (Penaeus monodon)

    International Nuclear Information System (INIS)

    Two lots of frozen precooked shrimps from Thailand ''PENAEUS MONODON'' Black tiger variety were irradiated at 1.8 to 3.6 kGy. This way, it was hoped to compare the effects of gamma irradiation on the microbiological quality and the organoleptic properties of frozen precooked shrimps after transportation from Thailand to Canada. The results indicated that the extension of shelf-life based on mesophiles content was from 33 days for the control to more than 47 days for the irradiated shrimps stored at 3 ± 1oC. The results of sensory evaluation gave slightly fresher odor for the control than the irradiated shrimps. On day one, this effect was more apparent. The results of hedonic tests showed that the irradiated shrimps were acceptable during storage. In conclusion, the results demonstrate that the combined treatments (freezing plus irradiation) of precooked shrimps are useful for increasing the storage life of shrimps without affecting consumer acceptability. (Author)

  4. DETECTED OF AERO GENE IN AEROMONAS HYDROPHILA ISOLATES FROM SHRIMP AND PEELED SHRIMP SAMPLES IN LOCAL MARKETS

    Directory of Open Access Journals (Sweden)

    Alaa Kareem Niamah

    2012-10-01

    Full Text Available A 62 isolates were isolated from 33 samples of shrimp and peeled shrimp in three local markets in Basrah city. A 36 isolates were identified to be Aeromonas hydrophila bacteria depending on morphological, microscopic examinations and ten biochemical tests. 83.33% (30 isolates from isolates produced ß-haemolysis on tryptone soy agar with 5% sheep blood. Aero gene detection by polymerase chain reaction technique, Theband appearance in the amplified gene of bacteria shows the molecular weight of aerolysin (424 bp. A 100% (36 isolates of Aeromonas hydrophila isolates were contained Aero gene.

  5. IMPACT OF PARASITISM BY GREGARINES (Nematopsis sp IN FARMING SHRIMP Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Francisco M. Guzmán-Sáenz

    2014-07-01

    Full Text Available Infestation of gregarines from genus Nematopsis in Pacific white shrimp Litopenaeus vannamei is commonly associated with a decrease in production and low weight, as well as a possible predisposition to viral infections. The goal of our past research was to evaluate the effect of the Nematopsis sp. gregarine parasitosis on the growth of farm-grown Litopenaeus vannamei shrimp. Four cages were built with PVC pipes and plastic screens with a 6mm light diameter, 1,7m in height, and 1,5m in width and length (2,23 m3, and were installed in a farm-growing area in La Pesca, Tamaulipas. Twenty-nine previously weighed shrimp, L. vannamei, were placed in each cage. These had a “2” (6 to 10 parasites per shrimp degree of infestation severity by Nematopsis sp. The shrimp in 2 cages were fed for 5 days with the farm feed and 6g/kg of sodium monensin. The shrimp in the remaining cages were fed with commercial feed. After 5 days, the shrimp in the 4 cages were fed with a balanced feed for 38 days. After this, the biomass of each cage was recorded. The shrimp in the control cages weighed an average of 7,12g at the beginning of research and 9,27g at the end, and the treated shrimp weighed 7,09g and 9,88g, respectively. The non-treated shrimp gained 2,5g and the treated shrimp gained 2,79g. This present research shows that the negative effect on the shrimp growth caused by Nematopsis sp. infestation can be reversed if the infected shrimp are treated against infestation with sodium monensin, which possesses a therapeutic effect that eliminated the infestation without affecting the shrimp growth.

  6. Absorption and retention of selenium from shrimps in man

    DEFF Research Database (Denmark)

    Bugel, S. H.; Sandstrom, B.; Larsen, Erik Huusfeldt

    2001-01-01

    This study was undertaken to evaluate the bioavailability of selenium in shrimps, a possible good source of selenium, by measurements of the absorption and retention of selenium and the effects on plasma selenium concentration and glutathione peroxidase activity. Twelve healthy young subjects (9F...... commencement of the study, after 2, 4, and 6 weeks. The selenium intake increased from 39.4 +/- 15.3 mug/d to 127 +/- 5.5 mug/d with the addition of shrimps. The apparent absorption of selenium from shrimps was 83 +/- 4%, Faecal and urinary selenium excretion was 32.5 +/- 17.0 mug/d and 21.2 +/- 9.0 mug/d, re...... spectively and the total retention of selenium was 3.1 +/- 1.1 mg. Plasma selenium concentrations were 95.2 +/- 9.7 mug/L and 101.5 +/- 9.7 mug/L before and after six weeks of shrimp intake, respectively (p...

  7. Halogenated contaminants in farmed salmon, trout, tilapia, pangasius, and shrimp.

    Science.gov (United States)

    van Leeuwen, S P J; van Velzen, M J M; Swart, C P; van der Veen, I; Traag, W A; de Boer, J

    2009-06-01

    Polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins and dibenzo-p-furans (PCDD/Fs), organochlorine pesticides (OCPs), polybrominated diphenyl ethers (PBDEs), hexabromocyclododecane diastereomers (HBCDs), and perfluorinated compounds (PFCs) were analyzed in popular farmed fish such as salmon, trout, tilapia, and pangasius and in farmed shrimp. The samples originated from southeast Asia, Europe, and South America. Results show the following: (i) Carnivorous species contained higher contaminant concentrations than omnivorous species. (ii) Contaminant concentrations generally decreased per species in the following order of salmon > trout > tilapia approximately equal to pangasius approximately equal to shrimp. (iii) Most contaminant concentrations decreased in the following order of PCBs approximately equal to dichloro-diphenyl-trichloroethanes (DDTs) > hexachlorobenzene approximately equal to pentachlorobenzene approximately equal to dieldrin approximately equal to PBDEs approximately equal to alpha-HBCD approximately equal to perfluorooctane sulfonate (PFOS) > World Health Organization toxic equivalents (WHO-TEQ) [PCDD/Fs and dioxin-like (dl)-PCBs]. (iv) Contaminant concentrations were very low (mostly <1 ng/g wet weight) and far below the European and Dutch legislative limits. (v) Contaminant concentrations in farmed shrimp, pangasius, and tilapia were lower than those in wild fish, whereas contaminant concentrations in farmed salmon and trout were higher than those in lean wild marine fish. From the five species investigated, salmon is predominantly responsible (97%) for human exposure to the sum of the investigated contaminants. The contribution of trout, tilapia, pangasius, and shrimp is small (3%) because contaminant concentrations and consumption volumes were much lower. PMID:19569323

  8. 77 FR 27411 - Sea Turtle Conservation; Shrimp Trawling Requirements

    Science.gov (United States)

    2012-05-10

    ... presenting various approaches to regulating trawl fisheries in the Atlantic Ocean (74 FR 21627). The scoping... hard TEDs conforming to a generic description and one type of soft TED--the Parker soft TED (see 50 CFR... shrimp fisheries and the skills required in their use are consistent with the skill set and...

  9. Optimization of carotenoids extraction from Penaeus semisulcatus shrimp wastes

    Directory of Open Access Journals (Sweden)

    Gholamreza jahed Khaniki

    2013-09-01

    Full Text Available Objective: To find effective method for carotenoids extraction from shrimp waste which is one of the important sources of natural carotenoids and produced in large quantities in Iran. Methods: Two methods of carotenoids extraction, enzymatic and alkaline (NaOH 1 normal treatment, were assayed. About 5 g of gritted shrimp wastes were used at each stage. For alkaline treatment, sodium hydroxide were added to shrimp waste. After 48 h, the mixture was filtered and centrifuged. Results: Alcalase extraction produced (234.00±2.00 mg/L carotenoid and NaOH extraction produced (170.00±1.53 mg/L carotenoid. Based on the samples analyzed, alcalase enzyme showed more efficiency than NaOH extraction to achieve carotenoids from shrimp waste. Conclusions: It can be concluded that using alcalase enzyme for carotenoids extraction can produce higher carotenoids concentration than NaOH extraction method. So alcalase enzyme method can be used for achieving this kind of antioxidant.

  10. On the vaccination of shrimp against white spot syndrome virus

    NARCIS (Netherlands)

    Witteveldt, J.

    2006-01-01

    More than a decade after its discovery inSouth-East Asia, White Spot Syndrome Virus (WSSV) is still the most important (viral) pathogen in the shrimp culture industry. Despite the shift from culturingPenaeusmonodon

  11. THE BRINE SHRIMP (ARTMA SALINA) LETHALITY OF SOME FERULAGO SPECIES

    OpenAIRE

    F. HIRLAK, S. DOĞANCA, E. GÜRKAN, O.T. TÜZÜN,

    2015-01-01

    In this work the bio-activities of the extracts obtained from F. asparagifoliaBoiss., F. humilis Boiss., F. aucheri Boiss. (Umbelliferae) are investigatedusing the Brine shrimp method.Key words: Ferulago aucheri, F. humilis, F. asparagifolia, Brineshrimp (Artemia salina).

  12. Bringing Scientific Inquiry Alive Using Real Grass Shrimp Research

    Science.gov (United States)

    Aultman, Terry; Curran, Mary Carla; Partridge, Michael

    2010-01-01

    This lesson was developed for middle school students using actual research on grass shrimp ("Palaemonetes pugio") to illustrate the process of a scientific investigation. The research was conducted at Savannah State University and funded by the National Oceanic and Atmospheric Administration (NOAA) Office of Education through the Living Marine…

  13. A Circo-Like Virus Isolated from Penaeus monodon Shrimps.

    OpenAIRE

    Pham, Hanh T.; Yu, Qian; Boisvert, Maude; Van, Hanh T; Bergoin, Max; Tijssen, Peter

    2014-01-01

    A virus with a circular Rep-encoding single-stranded DNA (ssDNA) (CRESS-DNA) genome (PmCV-1) was isolated from Penaeus monodon shrimps in Vietnam. The gene structure of the 1,777-nucleotide (nt) genome was similar to that of circoviruses and cycloviruses, but the nucleic acid and protein sequence identities to these viruses were very low.

  14. Haemocytic defence in black tiger shrimp (Penaeus monodon)

    NARCIS (Netherlands)

    Braak, van de C.B.T.

    2002-01-01

    Tropical shrimp culture is one of the fastest growing aquaculture sectors in the world. Since this production sector is highly affected by infectious pathogens, disease control is nowadays a priority. Effective prevention methods can be developed more efficiently when quantitative assays for the eva

  15. Talking about Brine Shrimps: Three Ways of Analysing Pupil Conversations.

    Science.gov (United States)

    Tunnicliffe, Sue Dale; Reiss, Michael J.

    1999-01-01

    Applies three distinct analyses to recorded and transcribed student conversations (n=240) about brine shrimps. The complementary analytic methods provide information on the content of pupils' conversations in terms of the observations made, the ways in which pupils make sense of their observations, and the ways in which students use conversation…

  16. Brine Shrimp and Their Habitat, An Environmental Investigation.

    Science.gov (United States)

    National Wildlife Federation, Washington, DC.

    This environmental unit is one of a series designed for integration within the existing curriculum. The unit is self-contained and students are encouraged to work at their own speed. The philosophy of the unit is based on an experience-oriented process that encourages independent student work. This unit explores the life cycle of brine shrimp and…

  17. Soybean meal, distillers grains replace fishmeal in experimental shrimp diets

    Science.gov (United States)

    The objective of this study was to evaluate inclusion of distiller’s dried grains with solubles (DDGS) as partial replacement of commercial, solvent-extracted soybean meal (SBM) in fish meal-free diets for Pacific white shrimp, Litopenaeus vannamei. Aquaria connected to a recirculating biofiltratio...

  18. Absorption and retention of selenium from shrimps in man

    DEFF Research Database (Denmark)

    Bugel, S. H.; Sandstrom, B.; Larsen, Erik Huusfeldt

    2001-01-01

    and 3M) received 100 g of shrimps each day for six weeks in addition to their habitual diet. In the third week of the study a balance period was inserted in which the subjects received all food from the department and collected faeces and urine over 5 days. Blood sam ples were collected at...

  19. Optimization of carotenoids extraction from Penaeus semisulcatus shrimp wastes

    Institute of Scientific and Technical Information of China (English)

    Gholamreza jahed Khaniki; Parisa Sadighara; Ramin Nabizadeh Nodehi; Mahmood Alimohammadi; Naiema Vakili Saatloo

    2013-01-01

    Objective: To find effective method for carotenoids extraction from shrimp waste which is one of the important sources of natural carotenoids and produced in large quantities in Iran. Methods: Two methods of carotenoids extraction, enzymatic and alkaline (NaOH 1 normal) treatment, were assayed. About 5 g of gritted shrimp wastes were used at each stage. For alkaline treatment, sodium hydroxide were added to shrimp waste. After 48 h, the mixture was filtered and centrifuged.Results:Alcalase extraction produced (234.00±2.00) mg/L carotenoid and NaOH extraction produced (170.00±1.53) mg/L carotenoid. Based on the samples analyzed, alcalase enzyme showed more efficiency than NaOH extraction to achieve carotenoids from shrimp waste.Conclusions:It can be concluded that using alcalase enzyme for carotenoids extraction can produce higher carotenoids concentration than NaOH extraction method. So alcalase enzyme method can be used for achieving this kind of antioxidant.

  20. Issues, impacts, and implications of shrimp aquaculture in Thailand

    Science.gov (United States)

    Dierberg, Forrest E.; Kiattisimkul, Woraphan

    1996-09-01

    Water quality impacts to and from intensive shrimp aquaculture in Thailand are substantial. Besides the surface and subsurface salinization of freshwaters, loadings of solids, oxygen-consuming organic matter, and nutrients to receiving waters are considerable when the cumulative impacts from water exchange during the growout cycle, pond drainage during harvesting, and illegal pond sediment disposal are taken into account. Although just beginning to be considered in Thailand, partial recirculating and integrated intensive farming systems are producing promising, if somewhat limited, results. By providing on-site treatment of the effluent from the shrimp growout ponds, there is less reliance on using outside water supplies, believed to be the source of the contamination. The explosion in the number of intensively operated shrimp farms has not only impacted the coastal zone of Thailand, but has also resulted in an unsustainable aquaculture industry. Abandonment of shrimp ponds due to either drastic, disease-caused collapses or more grandual, year-to-year reductions in the productivity of the pond is common. To move Thailand towards a more sustainable aquaculture industry and coastal zone environment, integrated aquaculture management is needed. Components of integrated aquaculture management are technical and institutional. The technical components involve deployment of wastewater treatment and minimal water-use systems aimed at making aquaculture operations more hydraulically closed. Before this is possible, technical and economic feasibility studies on enhanced nitrification systems and organic solids removal by oxidation between production cycles and/or the utilization of plastic pond liners need to be conducted. The integration of semi-intensive aquaculture within mangrove areas also should be investigated since mangrove losses attributable to shrimp aquaculture are estimated to be between 16 and 32% of the total mangrove area destroyed betweeen 1979 and 1993

  1. GROWING NIPA PALM FOR RESTORATION OF ABANDONED SHRIMP PONDS

    Institute of Scientific and Technical Information of China (English)

    Noparat Bamroongrugsa; Chorthip Purintavarakul

    2006-01-01

    Rapid increase in the production of marine shrimps in the coastal zone has resulted in large - scale conversion of mangrove forests to shrimp ponds. Productions of the shrimps in some regions have been unstable. Consequently, a number of ponds are left idle. Restoring environmental conditions within the pond site by planting a suitable mangrove species considered an option. The experiment herein was carried out to explore the effect of planting methods on growth of Nipa palm in abandoned shrimp ponds in the Southern Thailand. Economically, coastal villagers in this area earn their income from the palm in several ways, the most importantly in Nipa sugar production. Most seedlings were planted on top of furrows were constructed in the pond to avoid flooding, except for some seedlings which were planted in the pond base where additional pond sludge were slightly filled up to compare their growth rates. The results showed that planting seedlings with attached rhizomes had the best growth rate and without seedling death at 14 months after planting. At this stage, however, the height of seedlings grown from the fallen fruits was similar to those growth from bare rooted seedlings (70 cm tall). Also, seedling mortality was not observed when growth from fallen fruits whereas about6% mortality was experienced by the bare rooted ones. Among the bare rooted seedling treatments, however no significant differences in growth rates were found when applying the soil with chemical fertilizers or farm manure or liming with CaCO3. In addition, it was found that seedling growth measured at 40 months after planting for those growths in pond base was almost 100% greater than those grown on the furrows. Therefore, this finding may be applied for the restoration of abandoned shrimp ponds by growing Nipa palm.

  2. Accumulation of pesticide residues by shrimp, fish and brine shrimp during pond culture at Ghorabari (District Thatta)

    International Nuclear Information System (INIS)

    Residual level of persistent organo chlorines (OC) such as sigma-HCH (alpha-HCH, beta-HCH, gamma-HCH, delta-HCH, sigma-DDT (o,p'-DDD, op-DDE, p,p-DDE pp-DDD, pp'-DDT, o,p'-DDT), dieldrin and endrin were measured in a number of water samples from Ambro creek and their accumulation in shrimp (Penaeus merguiensis and P. penicillatus), fish (Otolithes ruber) and brine shrimp (Artemia sp.) reared in ponds for a period of four months. Samples were extracted with organic solvents, and quantified using gas chromatography-electron capture detection (GC/ECD). It has been found that results of animal tissue and water are not same however OCs, (mainly sigma DT and beta-HCH 4,4-DDT, Dieldrin + 2,4-DDT, and Methoxychlor were detected in all samples ). Heptachlor exo-epoxide were found in fish and Artemia sp. and absent in all shrimp samples. Heptachlor endo-epoxide was detected only in Artemia sp. and average residual concentration of OCs in Artemia sp. was 0.004-0.09 ppm. Methoxychlor was found in the highest quantity in all the samples whether it was fish, shrimp or Artemia. In fish average residual concentration of all (OCs) in individual sample was 0.03 - 0.180 ppm. (author)

  3. Chinese Dream

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    The general managers of South Korean auto giants Hyundai and Kia have high hopes for the growing Chinese auto market. Both companies went through a painstaking period as the financial crisis first roared across the globe. Jin Shan-fa, General Manager of Hyundai Motor Group

  4. An investigation on the application of ohmic heating of cold water shrimp and brine mixtures

    DEFF Research Database (Denmark)

    Pedersen, Søren Juhl; Feyissa, Aberham Hailu; Brøkner Kavli, Sissel Therese;

    2016-01-01

    Cooking is an important unit-operation in the production of cooked and peeled shrimps. The present study explores the feasibility of using ohmic heating for cooking of shrimps. The focus is on investigating the effects of different process parameters on heating time and quality of ohmic cooked...... shrimps (Pandalus Borelias). The shrimps were heated to a core temperature of 72 °C in a brine solution using a small batch ohmic heater. Three experiments were performed: 1) a comparative analyses of the temperature development between different sizes of shrimps and thickness (head and tail region...... of the shrimp) over varying salt concentrations (10 kg m−3 to 20 kg m−3) and electric field strengths (1150 V m−1 to 1725 V m−1) with the heating time as the response; 2) a 2 level factorial experiment for screening the impact of processing conditions using electric field strengths of 1250 V m−1 and 1580 V m−1...

  5. The effect of gamma irradiation on physical and nutritional quality of Penaeus Monodon shrimps

    International Nuclear Information System (INIS)

    Two lots of frozen precooked shrimps from Thailand ''PENAEUS MONODON'' Black tiger variety were irradiated at 1.8 to 3.6 kGy. This way, it was hoped to compare the effects of gamma irradiation on the chemical and physical qualities of frozen precooked shrimps after transportation from Thailand to Canada. The results indicated that the color measurement expressed in terms of lightness (L*) showed a relative stability during storage of processed shrimps. However, based on the value (θ) a more intense red color was observed for the control and the reference. The cooked irradiated and nonirradiated shrimps presented a significant decrease (p ≤ 0.01) of 22% in firmness during the first week of storage. Thus, irradiation did not impair the firmness of shrimps after treatment or during storage. Also, based on the statistical analysis, the irradiation treatment did not affect the content of amino acids of precooked shrimps. (Author)

  6. Autonomous water-cleaning machine using solar energy in shrimp ponds

    OpenAIRE

    Dang, Thien Ngon

    2012-01-01

    Limited water exchange shrimp culture technology is commonly used today in many shrimp farms in Vietnam to reduce water usage, input of diseases and discharge of nutrient-rich effluents into environment as well as to increase the production per unit area. However, a remaining problem in this technology is that the water quality in shrimp ponds will be reduced due to limitation of water exchange for a long period. The accumulation of inorganic components such as waste feed, bacterial deposits ...

  7. Econometric analysis on economies of scale: An application to rice and shrimp production in Thailand

    OpenAIRE

    Mekhora, Thamrong

    1999-01-01

    Shrimp production in Thailand has historically been undertaken in the saline and brackish waters of coastal mangroves. In recent years rising demand and prices for shrimp and falling productivity of mangrove areas have motivated an expansion of shrimp production into the fresh-water margins of river estuaries that were previously used for rice cultivation. Generalised additive models, which offer a comprehensive approach to regression analysis, are mainly used for empirical analysis, and mode...

  8. Knowledge and innovation relationships in the shrimp industry in Thailand and Mexico

    OpenAIRE

    Lebel, Louis; Garden, Po; Luers, Amy; Manuel-Navarrete, David; Giap, Dao Huy

    2009-01-01

    Experts, government officials, and industry leaders concerned about the sustainability of shrimp aquaculture believe they know what farmers need to know and should be doing. They have framed sustainability as a technical problem that, at the farm level, is to be solved by better shrimp and management of ponds and businesses. Codes of conduct, standards, and regulations are expected to bring deviant practices into line. Shrimp farmers are often cornered in a challenging game of knowledge in wh...

  9. Research on Surimi Beat Break Parameters and Shaping Process for Simulation Products of Shelled Shrimps

    Directory of Open Access Journals (Sweden)

    Wei He

    2015-07-01

    Full Text Available This study describes the production process of simulation products of shelled shrimps. Through the determination experiment of gel strength, the process parameter, the optimal period of beat break, is drawn in white silver carp surimi beat break which is suitable for simulation shelled shrimps. Using CATIA V5 R16, reverse design and manufacture of simulation shelled shrimps mould die is completed.

  10. Molecular epidemiology of Vibrio nigripulchritudo, a pathogen of cultured penaeid shrimp (Litopenaeus stylirostris) in New Caledonia

    OpenAIRE

    Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Saulnier, Denis; Leroux, Frederique

    2006-01-01

    A collection of 57 isolates of Vibrio nigripulchritudo from either diseased or healthy shrimp and from shrimp farms environment was studied in order to gain a better understanding of the epidemiology of this pathogen, notably isolated from two distinct shrimp disease complexes. Molecular typing using two different techniques, arbitrarily primed PCR (AP-PCR) and multi-locus sequence typing (MLST), studied together with experimental pathology data allowed a relevant epidemiological insight into...

  11. The American brine shrimp as an exotic invasive species in the western Mediterranean

    OpenAIRE

    Amat, Francisco; Hontoria, Francisco; Ruiz, Olga; Green, Andy J.; Sánchez, Marta I.; Figuerola, Jordi; Hortas, F.

    2005-01-01

    The hypersaline environments and salterns present in the western Mediterranean region (including Italy, southern France, the Iberian Peninsula and Morocco) contain autochthonous forms of the brine shrimp Artemia, with parthenogenetic diploid and tetraploid strains coexisting with the bisexual species A. sal- ina. Introduced populations of the American brine shrimp A. franciscana have also been recorded in these Mediterranean environments since the 1980s. Based on brine shrimp cyst samples...

  12. Ecological limitations and appropriation of ecosystem support by shrimp farming in Colombia

    Science.gov (United States)

    Larsson, Jonas; Folke, Carl; Kautsky, Nils

    1994-09-01

    Shrimp farming in mangrove areas has grown dramatically in Asia and Latin America over the past decade. As a result, demand for resources required for farming, such as feed, seed, and clean water, has increased substantially. This study focuses on semiintensive shrimp culture as practiced on the Caribbean coast of Colombia. We estimated the spatial ecosystem support that is required to produce the food inputs, nursery areas, and clean water to the shrimp farms, as well as to process wastes. We also made an estimate of the natural and human-made resources necessary to run a typical semiintensive shrimp farm. The results show that a semiintensive shrimp farm needs a spatial ecosystem support—the ecological footprint—that is 35-190 times larger than the surface area of the farm. A typical such shrimp farm appropriates about 295 J of ecological work for each joule of edible shrimp protein produced. The corresponding figure for industrial energy is 40:1. More than 80% of the ecological primary production required to feed the shrimps is derived from external ecosystems. In 1990 an area of 874-2300 km2 of mangrove was required to supply shrimp postlarvae to the farms in Colombia, corresponding to a total area equivalent to about 20-50% of the country’s total mangrove area. The results were compared with similar estimates for other food production systems, particularly aquacultural ones. The comparison indicates that shrimp farming ranks as one of the most resource-intensive food production systems, characterizing it as an ecologically unsustainable throughput system. Based on the results, we discuss local, national, and regional appropriation of ecological support by the semiintensive shrimp farms. Suggestions are made for how shrimp farming could be transformed into a food production system that is less environmentally degrading and less dependent on external support areas.

  13. Testing for Market Integration and the Law of One Price in World Shrimp Markets

    OpenAIRE

    Vinuya, Ferdinand D.

    2006-01-01

    Using import price data from Japan, United States, and the European Union, we test if shrimp price movements in these markets indicate an integrated world market for shrimp. We utilize cointegration techniques to investigate if prices in these markets share a common stochastic trend and if the law of one price holds. Results point to strong linkage among Japanese, American, and European markets. The results on the aggregated shrimp markets are checked against the results at a more disaggregat...

  14. Potentials of converting microalgae into brine shrimp Artemia

    OpenAIRE

    Sorgeloos, P

    1985-01-01

    High densities of brine shrimp Artemia can be cultured in flow-through systems using the effluent of microalgae cultures as a combined source of culture medium and food. It has been proven at the "St. Croix Artificial Upwelling Mariculture Project" that in comparison with the fast growing clam Tapes japonica, brine shrimp assure a much more efficient conversion of plant into animal biomass. It appears from the given examples that microalgae conversion into brine shrimp Artemia offers efficien...

  15. Effects of low molecular weight chitosan (LMC-1) on shrimp preservation

    Science.gov (United States)

    Yu, Guang-Li; Wang, Yuan-Hong; Liu, Shu-Qing; Tian, Xue-Lin

    1996-06-01

    This study on the effects of low molecular weight chitosan (LMC-1) and shrimp preserving agents such as phytic acid (PA), sodium bisulfite (SB), and crustacean preservative (CP) on the preservation of shrimp ( Trachypenaeus curvirostris) and the bacteriostasis of LMC-1 showed that: (1) Different LMC-1 concentration has different bacteriostasis on E. coli, B. subtilis and S. aureau; (2) LMC-1 and CP are better than PA and SB for preserving the freshness of shrimp stored at 4 °C.

  16. An Economic Analysis of Shrimp Farm Expansion and Mangrove Conversion in Thailand

    OpenAIRE

    Barbier, Edward B.; Mark Cox

    2004-01-01

    We analyzed the factors determining mangrove conversion by shrimp farmers in the coastal provinces of Thailand between 1979–1996. The profit-maximizing behavior of a shrimp farmer converting mangrove area under open access conditions is modeled. Aggregate mangrove clearing by all farmers in coastal areas is empirically estimated through a provincial-level panel analysis. The price of shrimp, minimum wage, distance from market, ammonium phosphate price (used for feed), population growth, gross...

  17. The Effect of Various Methods of Defrosting on Microbial Contamination of Frozen Banana Shrimp (Penaeus merguiensis)

    Institute of Scientific and Technical Information of China (English)

    Ladan Mansouri-Najand

    2012-01-01

    Objective: Background and aim: The most common and the best way of preventing microbial decay of marine foods is using freezing technology and the cycles and methods of defrosting have considerable effect on microbial changes of frozen shrimp. Shrimp is one of the marine foods that due to high active water (aw) and neutral PH and autolytic enzymes have high decay. Thus, in this study the effect of various methods of defrosts on microbial contamination of shrimp was investigated. Methods: This study was an empirical design on Penaeus merguiensis. The shrimps were divided into three groups including 1- peeled and headless (PUD), 2- Complete, 3- Headless (with skin) being frosted and defrosted in three cycles. Each group of shrimp was classified in terms of the type of defrosting method in three groups as 1- Microwave, 2- Refrigerator, 3-Water and were investigated in 3 cycles with the interval of 4 days. In this investigation, the total bacteria, Psychrophil bacteria, coliforms and Staphylococcus aureus were counted in private culture mediums. For data analysis, repeated measure Anova was used. Results: All the bacteria including Psychrophil bacteria, coliforms and Staphylococcus aureus during the cycles had significant reduction process and this reduction showed significant reduction in complete shrimp and defrosting with refrigerator compared to other groups (P<0.05). Conclusions: According to the results, complete shrimp was the best kind of shrimp in terms of microbial load. Thus, avoiding temperature changes during transportation and avoiding unduly defrosts in maintaining the quality of the frozen shrimp is proposed.

  18. Combined effect of external ammonia and molt stage on the blue shrimp Litopenaeus stylirostris physiological response

    OpenAIRE

    Mugnier, Chantal; Justou, Carole

    2004-01-01

    The effect of ambient ammonia and molt stage on the physiological response of the blue shrimp Litopenaeus stylirostris was studied. Shrimps were submitted to 54.6 mg l(-1) ammonia-N (1.76 mg l(-1) NH3-N) for 24 h. Only shrimps in stages C, D-0, D-1 and D-2 were used for the analysis. Haemolymph was assayed for osmoregulatory capacity (OC), magnesium ion (Mg ion), total proteins, oxyhaemocyanin, lactate and glucose. Molt stage had an effect on OC, Mg ion and total proteins in control shrimps, ...

  19. Social and ecological challenges of market-oriented shrimp farming in Vietnam.

    Science.gov (United States)

    Lan, Ngo Thi Phuong

    2013-01-01

    Vietnam is one of the largest shrimp exporters in the world. Since 2010, Vietnam has earned about two billion dollars annually through shrimp exports. As a fertile area of greatest potential for agricultural production in Vietnam, the Mekong Delta has been a major contributor to the country's achievements, especially in the agricultural sector. During recent decades, trade liberation along with various policies in support of aquaculture has accelerated the development of shrimp production in the Delta. Based on an ethnographic study of shrimp farming in the Mekong Delta of Vietnam, I assert that along with great rewards arising from the expansion of shrimp farming areas, productivity, and export value, the shrimp industry has brought various environmental, economic and social challenges. Consequently, shrimp farming is a risky business and local inhabitants have relied on various strategies to cope with these challenges. Risk mitigation in shrimp production and labor migration are the two important strategies of local inhabitants for securing their livelihoods. Water pollution and poor quality post-larvae shrimp are direct consequences of market-oriented production. PMID:24386621

  20. Production and characterization of Chitosan from shrimp shells waste

    Directory of Open Access Journals (Sweden)

    Anshar Patria

    2013-07-01

    Full Text Available This research aims to study the production of chitosan from shrimp shell waste origin andcharacterize the chitosan quality of shrimp shell waste that includes parameters yield, solubility,intrinsic viscosity, molecular weight and deacetylation degree. Results showed that the treatment ofheating temperature and heating time in the process of deacetylation caused significant (P≤0.01influence on yield, solubility, intrinsic viscosity, molecular weight and deacetylation degree of producedchitosan. Whereas, the interaction between heating temperature and heating time on the process ofdeacetylation gave no significant effect on yield, solubility, intrinsic viscosity, molecular weight anddeacetylation degree of chitosan produced. The best results in this study were obtained from heatingtemperature of 100˚C and 80 min heating time.

  1. Guiding brine shrimp through mazes by solving reaction diffusion equations

    Science.gov (United States)

    Singal, Krishma; Fenton, Flavio

    Excitable systems driven by reaction diffusion equations have been shown to not only find solutions to mazes but to also to find the shortest path between the beginning and the end of the maze. In this talk we describe how we can use the Fitzhugh-Nagumo model, a generic model for excitable media, to solve a maze by varying the basin of attraction of its two fixed points. We demonstrate how two dimensional mazes are solved numerically using a Java Applet and then accelerated to run in real time by using graphic processors (GPUs). An application of this work is shown by guiding phototactic brine shrimp through a maze solved by the algorithm. Once the path is obtained, an Arduino directs the shrimp through the maze using lights from LEDs placed at the floor of the Maze. This method running in real time could be eventually used for guiding robots and cars through traffic.

  2. Shrimp Lipids: A Source of Cancer Chemopreventive Compounds

    Directory of Open Access Journals (Sweden)

    Armando Burgos-Hernández

    2013-10-01

    Full Text Available Shrimp is one of the most popular seafoods worldwide, and its lipids have been studied for biological activity in both, muscle and exoskeleton. Free fatty acids, triglycerides, carotenoids, and other lipids integrate this fraction, and some of these compounds have been reported with cancer chemopreventive activities. Carotenoids and polyunsaturated fatty acids have been extensively studied for chemopreventive properties, in both in vivo and in vitro studies. Their mechanisms of action depend on the lipid chemical structure and include antioxidant, anti-proliferative, anti-mutagenic, and anti-inflammatory activities, among others. The purpose of this review is to lay groundwork for future research about the properties of the lipid fraction of shrimp.

  3. Chinese restaurant syndrome

    Science.gov (United States)

    Chinese restaurant syndrome is a set of symptoms that some people have after eating Chinese food. A food additive ... Chinese restaurant syndrome is most often diagnosed based on the symptoms. The health care provider may ask the following ...

  4. CHINESE JOURNAL OF CHEMISTRY

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    @@Chinese Journal of Chemistry is an international journal published in English by the Chinese Chemical Society with its editorial office hosted by Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences.

  5. Chinese Culture and Leadership.

    Science.gov (United States)

    Wong, Kam-Cheung

    2001-01-01

    Describes essential characteristics of Chinese philosophical tradition; Discusses Western perspectives on value leadership in education, particularly moral leadership. Discuses moral leadership from a Chinese philosophical perspective, especially Confucianism. Draws implications for using Chinese cultural and philosophical traditions to develop…

  6. Brine shrimp lethality bioassay of selected Centaurea L. species (Asteraceae)

    OpenAIRE

    Janaćković P.; Tešević V.; Marin P.D.; Milosavljević S.; Duletić-Laušević Sonja; Janaćković Slavica; Veljić M.

    2008-01-01

    Ether extracts of 15 Centaurea L. species (Asteraceae) methanol extracts of 12species, and cnicin isolated from C. derventana were tested for general bioactivity using the brine shrimp lethality test. Cnicin showed the most potent activity with LC50 0.2. Also, ether extract of C. splendens showed significant activity with LC50 7.3, as did methanol extract of C. arenaria with LC50 12.4.

  7. Cyanobacterial cytotoxicity versus toxicity to brine shrimp Artemia salina

    Czech Academy of Sciences Publication Activity Database

    Hisem, D.; Hrouzek, Pavel; Tomek, P.; Tomšíčková, J.; Zapomělová, Eliška; Skácelová, K.; Lukešová, Alena; Kopecký, Jiří

    2011-01-01

    Roč. 57, č. 1 (2011), s. 76-83. ISSN 0041-0101 R&D Projects: GA ČR GAP504/10/1501 Institutional research plan: CEZ:AV0Z50200510; CEZ:AV0Z60170517; CEZ:AV0Z60660521 Keywords : Secondary metabolites * Brine shrimp bioassay * Health risk assessment Subject RIV: EE - Microbiology, Virology Impact factor: 2.508, year: 2011

  8. Hydraulic preferences of shrimps and fishes in tropical insular rivers

    OpenAIRE

    Girard, V.; Monti, D.; Valade, P.; Lamouroux, N.; Mallet, J.P.; Grondin, H.

    2014-01-01

    Hydraulic habitat models based on the preferences of species for the hydraulic characteristics of their microhabitats are frequently used to evaluate the impact on the habitat of a change in river flow regime. Their application in a tropical insular environment is still limited as little is known about the hydraulic preferences of species. Hydraulic preference models have been developed for 15 taxa (diadromous shrimps and fishes) sampled in 52 rivers in the Caribbean (the French West Indies) ...

  9. A Circo-Like Virus Isolated from Penaeus monodon Shrimps.

    Science.gov (United States)

    Pham, Hanh T; Yu, Qian; Boisvert, Maude; Van, Hanh T; Bergoin, Max; Tijssen, Peter

    2014-01-01

    A virus with a circular Rep-encoding single-stranded DNA (ssDNA) (CRESS-DNA) genome (PmCV-1) was isolated from Penaeus monodon shrimps in Vietnam. The gene structure of the 1,777-nucleotide (nt) genome was similar to that of circoviruses and cycloviruses, but the nucleic acid and protein sequence identities to these viruses were very low. PMID:24435870

  10. Market Integration for Shrimp and the Effect of Catastrophic Events

    OpenAIRE

    Harri, Ardian; Muhammad, Andrew; Jones, Keithly G.

    2010-01-01

    Seasonal unit-root testing and seasonal cointegration methods are employed to investigate the price transmission in U.S. shrimp markets. ARIMA and Vector Error Correction Models (VECM) are used to identify the effect of catastrophic events on individual price series in one region and the spillover effects in the price series for other regions. Results showed that a cointegrating relation exists between neighboring states, specifically between Alabama and Mississippi and Louisiana and Texas. C...

  11. Agro fertilizer from Myanmar traditional shrimp sauce and paste waste

    International Nuclear Information System (INIS)

    The new growth agro fertilizer (npi) compost prepared from Myanmar traditional shrimp sauce and paste waste mixed with other ingredients [agricultural waste and animal waste (night soil)] was found to promote and enhance the growth as well as the rice crop to produce in higher percentage yield per acre as compare to the use of normal rice crops fertilizer and even to that of the current used EM compost fertilizer. (author)

  12. Conservation by irradiation of the blanched and cooled shrimps

    International Nuclear Information System (INIS)

    In this work, we studied the gamma radiation influence on the initial reduction of contaminated germs and on physicochemical characteristics of shrimps storage and cooled at 21 days. These doses of 1.5; 2.5 and 3. 5 KGy proved insufficient to reduce in a significant way the initial rate of contamination mesophiles,, halophilous and psychrotrophes germs. 3.5 KGy was the most effectiveness. The contamination focal germs miss in the control samples. The toxinfections germs miss completely in all samples. In addition to the pH, the nitrogen basic volatile total and trimethylamine quantity increased during the cooled storage of shrimps. The capacity of water retention decreased indifferently by the doses applied. The irradiation made it possible to lengthen the shelf life of shrimps from 10 and 6 days respectively for 3,5 and 2,5 KGy. Finally, the irradiation doses did not affect neither the loss of weight during cooking neither the variations of pH at the end of dry and wet cooking.(author)

  13. The Chinese Banking System

    OpenAIRE

    Grant Turner; Nicholas Tan; Dena Sadeghian

    2012-01-01

    The Chinese banking system is critical to the functioning of the Chinese economy, being the main conduit through which savings are allocated to investment opportunities. Banking activity in China has grown rapidly over the past decade in association with the expansion of the Chinese economy, and the Chinese banking system now includes some of the world’s largest banks. Chinese banks have become more commercially orientated over this period, although the Chinese Government retains considerable...

  14. An investigation into mechanical strength of exoskeleton of hydrothermal vent shrimp (Rimicaris exoculata) and shallow water shrimp (Pandalus platyceros) at elevated temperatures

    Energy Technology Data Exchange (ETDEWEB)

    Verma, Devendra; Tomar, Vikas, E-mail: tomar@purdue.edu

    2015-04-01

    This investigation reports a comparison of the exoskeleton mechanical strength of deep sea shrimp species Rimicaris exoculata and shallow water shrimp species Pandalus platyceros at temperatures ranging from 25 °C to 80 °C using nanoindentation experiments. Scanning Electron Microscopy (SEM) observations suggest that both shrimp exoskeletons have the Bouligand structure. Differences in the structural arrangement and chemical composition of both shrimps are highlighted by SEM and EDX (Energy Dispersive X-ray) analyses. The variation in the elastic moduli with temperature is found to be correlated with the measured compositional differences. The reduced modulus of R. exoculata is 8.26 ± 0.89 GPa at 25 °C that reduces to 7.61 ± 0.65 GPa at 80 °C. The corresponding decrease in the reduced modulus of P. platyceros is from 27.38 ± 2.3 GPa at 25 °C to 24.58 ± 1.71 GPa at 80 °C. The decrease in reduced moduli as a function of temperature is found to be dependent on the extent of calcium based minerals in exoskeleton of both types of shrimp exoskeletons. - Highlights: • Shrimp species Pandalus platyceros and Rimicaris exoculata exoskeletons are analyzed. • Temperature dependent properties of shrimp exoskeleton are compared. • Mechanical properties are correlated with structure and composition of exoskeleton. • Mechanical properties reduce with increase in temperature. • Presence of biominerals gives better thermal stability to structure.

  15. An investigation into mechanical strength of exoskeleton of hydrothermal vent shrimp (Rimicaris exoculata) and shallow water shrimp (Pandalus platyceros) at elevated temperatures

    International Nuclear Information System (INIS)

    This investigation reports a comparison of the exoskeleton mechanical strength of deep sea shrimp species Rimicaris exoculata and shallow water shrimp species Pandalus platyceros at temperatures ranging from 25 °C to 80 °C using nanoindentation experiments. Scanning Electron Microscopy (SEM) observations suggest that both shrimp exoskeletons have the Bouligand structure. Differences in the structural arrangement and chemical composition of both shrimps are highlighted by SEM and EDX (Energy Dispersive X-ray) analyses. The variation in the elastic moduli with temperature is found to be correlated with the measured compositional differences. The reduced modulus of R. exoculata is 8.26 ± 0.89 GPa at 25 °C that reduces to 7.61 ± 0.65 GPa at 80 °C. The corresponding decrease in the reduced modulus of P. platyceros is from 27.38 ± 2.3 GPa at 25 °C to 24.58 ± 1.71 GPa at 80 °C. The decrease in reduced moduli as a function of temperature is found to be dependent on the extent of calcium based minerals in exoskeleton of both types of shrimp exoskeletons. - Highlights: • Shrimp species Pandalus platyceros and Rimicaris exoculata exoskeletons are analyzed. • Temperature dependent properties of shrimp exoskeleton are compared. • Mechanical properties are correlated with structure and composition of exoskeleton. • Mechanical properties reduce with increase in temperature. • Presence of biominerals gives better thermal stability to structure

  16. 78 FR 14069 - Fisheries of the Caribbean, Gulf of Mexico, and South Atlantic; Shrimp Fishery Off the Southern...

    Science.gov (United States)

    2013-03-04

    ... overfished and overfishing status determination criteria for pink shrimp. DATES: Written comments must be... closure. Overfished and Overfishing Status Determination Criteria for Pink Shrimp Amendment 9 would update the overfished and overfishing status determination criteria (biomass at maximum sustainable yield...

  17. Chromatographic, NMR and vibrational spectroscopic investigations of astaxanthin esters: application to "Astaxanthin-rich shrimp oil" obtained from processing of Nordic shrimps.

    Science.gov (United States)

    Subramanian, B; Thibault, M-H; Djaoued, Y; Pelletier, C; Touaibia, M; Tchoukanova, N

    2015-11-01

    Astaxanthin (ASTX) is a keto carotenoid, which possesses a non-polar linear central conjugated chain and polar β-ionone rings with ketone and hydroxyl groups at the extreme ends. It is well known as a super anti-oxidant, and recent clinical studies have established its nutritional benefits. Although it occurs in several forms, including free molecule, crystalline, aggregates and various geometrical isomers, in nature it exists primarily in the form of esters. Marine animals accumulate ASTX from primary sources such as algae. Nordic shrimps (P. borealis), which are harvested widely in the Atlantic Ocean, form a major source of astaxanthin esters. "Astaxanthin-rich shrimp oil" was developed as a novel product in a shrimp processing plant in Eastern Canada. A compositional analysis of the shrimp oil was performed, with a view to possibly use it as a nutraceutical product for humans and animals. Astaxanthin-rich shrimp oil contains 50% MUFAs and 22% PUFAs, of which 20% are omega-3. In addition, the shrimp oil contains interesting amounts of EPA and DHA, with 10%/w and 8%/w, respectively. Astaxanthin concentrations varied between 400 and 1000 ppm, depending on the harvesting season of the shrimp. Astaxanthin and its esters were isolated from the oil and analysed by NMR, FTIR and Micro-Raman spectroscopy. Astaxanthin mono- and diesters were synthesized and used as standards for the analysis of astaxanthin-rich shrimp oil. NMR and vibrational spectroscopy techniques were successfully used for the rapid characterization of monoesters and diesters of astaxanthin. Raman spectroscopy provided important intermolecular interactions present in the esterified forms of astaxanthin molecules. Also discussed in this paper is the use of NMR, FTIR and Micro-Raman spectroscopy for the detection of astaxanthin esters in shrimp oil. PMID:26393239

  18. 采用 OIE 标准检测养殖对虾中传染性皮下及造血组织坏死病毒(IHHNV)的 PCR 检出类型%PCR Detecting Types by the OIE Standards for Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) in Farmed Shrimp

    Institute of Scientific and Technical Information of China (English)

    袁颜颜; 杨冰; 万晓媛; 刘笋; 刘天齐; 黄倢

    2015-01-01

    Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is a kind of common virus in shrimp culture industry in China, influencing prawn breeding all over the world. IHHNV is harmful to Litopenaeus stylirostris and the mortality can be as high as 90%. For Litopenaeus vannamei, the mortality is not high, but can suffer from runt deformity syndrome (RDS). IHHNV infection in Penaeus monodon is usually subclinical, but RDS, reduced growth rates and reduced culture performance have been reported in IHHNV-infected stocks. OIE recommended 5 sets of primer pairs to detect different genotypes of IHHNV. In order to know the detecting types in China, we used four sets of primer pairs in the PCR protocols recommended by the OIE standards to detect penaeid shrimp samples collected in 2011 and 2012 from different provinces. These four sets of primer pairs are 389F/389R, 392F/392R, 77012F/77353R, 309F/309R, respectively. The detection results showed that IHHNV positives were detected in the samples of L. vannamei, P. monodon, Fenneropenaeus chinensis and Melicertus latisulcatus, but not in the sample of Palaemon carincauda. The positive percentage of L. vannamei was the highest, 70.8% (389F/R) in 2011 and 34.3% (389F/R) in 2012, and that of F. chinensis was the lowest, which is 8.3%. The more positive of penaeid shrimp samples in 2011 were detected than that in 2012. The positive percentage of IHHNV in penaeid shrimp samples from East China is higher than that from North China and South China. We got four detecting types from the PCR detection with 4 sets of primer pairs recommended by the OIE standards. For detected typeⅠ, the positive rate is 90% for all the four primer sets. For detected typeⅡ, the positive rate is 4% for primer set 389F/R. For detected type Ⅲ, the positive rate is 4% for primer sets of 389F/389R, 392F/392R and 309F/309R. For detected type Ⅳ, the positive rate is 2% for primer sets of 389F/389R and 309F/309R. The survey provided valuable

  19. Abundance and size of Gulf shrimp in Louisiana's coastal estuaries following the Deepwater Horizon oil spill.

    Directory of Open Access Journals (Sweden)

    Joris L van der Ham

    Full Text Available The Deepwater Horizon oil spill impacted Louisiana's coastal estuaries physically, chemically, and biologically. To better understand the ecological consequences of this oil spill on Louisiana estuaries, we compared the abundance and size of two Gulf shrimp species (Farfantepeneus aztecus and Litopeneus setiferus in heavily affected and relatively unaffected estuaries, before and after the oil spill. Two datasets were used to conduct this study: data on shrimp abundance and size before the spill were available from Louisiana Department of Wildlife and Fisheries (LDWF. Data on shrimp abundance and size from after the spill were independently collected by the authors and by LDWF. Using a Before-After-Control-Impact with Paired sampling (BACIP design with monthly samples of two selected basins, we found brown shrimp to become more abundant and the mean size of white shrimp to become smaller. Using a BACIP with data on successive shrimp year-classes of multiple basins, we found both species to become more abundant in basins that were affected by the spill, while mean shrimp size either not change after the spill, or increased in both affected and unaffected basins. We conclude that following the oil spill abundances of both species increased within affected estuaries, whereas mean size may have been unaffected. We propose two factors that may have caused these results: 1 exposure to polycyclic aromatic hydrocarbons (PAHs may have reduced the growth rate of shrimp, resulting in a delayed movement of shrimp to offshore habitats, and an increase of within-estuary shrimp abundance, and 2 fishing closures established immediately after the spill, may have resulted in decreased fishing effort and an increase in shrimp abundance. This study accentuates the complexities in determining ecological effects of oil spills, and the need of studies on the organismal level to reveal cause-and-effect relationships of such events.

  20. Experimental evaluation of co-culture of juvenile sea cucumbers, Holothuria scabra (Jaeger), with juvenile blue shrimp, Litopenaeus stylirostris (Stimpson)

    OpenAIRE

    Purcell, S.; Patrois, Jacques; Fraisse, N

    2006-01-01

    The co-culture of juvenile sea cucumber Holothuria scabra (Jaeger), or 'sandfish', with juvenile blue shrimp Litopenaeus stylirostris (Stimpson) was tested by growing groups in co-culture and monoculture for 3 weeks in tanks with enriched sand substratum. Feed was supplied on trays, accessible only to shrimp. Survival of shrimp and sandfish was high in all treatments (73-100%). Growth of shrimp did not differ between monoculture and co-culture, but sandfish grew significantly slower in co-cul...

  1. Impact of solid shrimp pond waste materials on mangrove growth and mortality: a case study from Pak Phanang, Thailand

    OpenAIRE

    Vaiphasa, C.; Boer, de, F.R.; A. K. Skidmore; Panitchart, S.; Vaiphasa, T.; Bamrongrugsa, N.; Santitamnont, P.

    2007-01-01

    One of the most serious threats to tropical mangrove ecosystems caused by shrimp farming activities is the poor management of pond wastematerials.We hypothesise thatmangroves can tolerate chemical residues discharged from shrimp farms and can be used as biofilters, but the capability of mangroves to cope with solid sediments dredged from shrimp ponds is limited. Our study in Pak Phanang, Thailand, confirmed that the excess sediments discharged from nearby shrimp ponds reduced mangrove growth ...

  2. 75 FR 53947 - Certain Frozen Warmwater Shrimp From Brazil, India, the People's Republic of China, Thailand, and...

    Science.gov (United States)

    2010-09-02

    ... Canned Warmwater Shrimp From Brazil, 69 FR 76910 (December 23, 2004) (``Brazil Final Determination... Warmwater Shrimp From the People's Republic of China, 69 FR 70997 (December 8, 2004) (``China Final... Fair Value and Antidumping Duty Order: Certain Frozen Warm water Shrimp from Brazil, 70 FR...

  3. Application of risk perception and communication strategies to manage disease outbreaks of coastal shrimp farming in developing countires

    DEFF Research Database (Denmark)

    Ahsan, Dewan

    2008-01-01

    farming like, production risks, technical risks, economical risks and disease of shrimp. However, risk of economic losses due to shrimp mortality (for diseases) is the major concern of the shrimp producers of Bangladesh, India, Thailand, China and many other countries. Poor water quality and high stocking...

  4. Inactivation of natural microflora and Listeria innocua on raw whole shrimp by ozonated water, antimicrobial coatings, and cryogenic freezing

    Science.gov (United States)

    Shrimp have been associated with foodborne illness outbreaks. A survey was conducted to investigate the microbiological safety and quality of frozen raw whole shrimp in local US markets. Results from 32 brands of raw whole shrimp samples available in local retail markets representing 9 countries of ...

  5. Chinese Calendar and Chinese Telegraphic Code.

    Science.gov (United States)

    Defense Language Inst., Monterey, CA.

    This manual contains: (1) Chinese calendars for the hundred years from 1881 to 1980; and (2) the Chinese telegraphic code. Each page in Part One presents the calendar for each year in both Chinese and English. There are 97 charts in Part Two representing the telegraphic code. (AMH)

  6. 77 FR 13275 - Certain Frozen Warmwater Shrimp From India: Preliminary Results of Antidumping Duty...

    Science.gov (United States)

    2012-03-06

    ...) expenses, profit, and U.S. packing costs. For each respondent, we calculated the cost of materials and... Warmwater Shrimp from India, 70 FR 5147 (Feb. 1, 2005) (Shrimp Order). \\2\\ See Antidumping or Countervailing Duty Order, Finding, or Suspended Investigation; Opportunity to Request Administrative Review, 76...

  7. 76 FR 12025 - Certain Frozen Warmwater Shrimp From India: Preliminary Results of Antidumping Duty...

    Science.gov (United States)

    2011-03-04

    ...) expenses, profit, and U.S. packing costs. For each respondent, we calculated the cost of materials and...: Certain Frozen Warmwater Shrimp from India, 70 FR 5147 (Feb. 1, 2005) (Shrimp Order). On February 1, 2010... Suspended Investigation; Opportunity to Request Administrative Review, 75 FR 5037 (Feb. 1, 2010)....

  8. 78 FR 50381 - Certain Frozen Warmwater Shrimp From Malaysia: Final Affirmative Countervailing Duty Determination

    Science.gov (United States)

    2013-08-19

    ...) Sdn. Bhd., and Charoen Pokphand Foods (Malaysia) Sdn. Bhd., (collectively, the Asia Aquaculture... Aquaculture), Star 10.80 Feedmills (M) Sdn. Bhd. (Star Feedmills), and Charoen Pokphand Foods (Malaysia) Sdn....; Indian Ridge Shrimp Co.; JBS Packing Co., Inc.; Lafitte Frozen Foods Corp.; M&M Shrimp (Biloxi...

  9. MODELING THE RELATIONSHIP BETWEEN SHRIMP MARICULTURE AND WATER QUALITY IN THE RIO CHONE ESTUARY, ECUADOR

    Science.gov (United States)

    The Rio Chone estuary in Ecuador has been heavily altered by the conversion of over 90% of the original mangrove forest to shrimp ponds. We carried out computational experiments using both hydrodynamic and shrimp pond models to investigate factors leading to declines in estuarine...

  10. 75 FR 1078 - Frozen Warmwater Shrimp From Brazil, China, India, Thailand, and Vietnam

    Science.gov (United States)

    2010-01-08

    ... warmwater shrimp from Brazil, China, India, Thailand, and Vietnam (70 FR 5143-5156).\\2\\ The Commission is... part 207), as most recently amended at 74 FR 2847 (January 16, 2009). \\1\\ No response to this request... shrimp from Thailand with respect to certain manufacturer/exporters. 74 FR 5638 (January 30, 2009)....

  11. Shipping trial of irradiated frozen shrimp from Thailand to the Netherlands

    International Nuclear Information System (INIS)

    Quality of non-irradiated and irradiated frozen shrimp after shipping trial from Thailand to the Netherlands were investigated. Commercial frozen shell on headless shrimp and pre-cooked and peeled shrimp were irradiated with 0, 2, and 3 kGy at the Office of Atomic Energy for Peace (OAEP), Thailand, and transported by sea to the International Facility for Food Irradiation Technology (IFFIT), the Netherlands. It was found that both types of frozen shrimp before shipment are of good quality. Radiation with 3 kGy resulted in 2-3 log cycles reduction of mesophilic and psychrotrophic colony counts and caused no significant change in sensory quality. The irradiated products were found to be free from coliforms, faecal coliforms, Escherichia coli, salmonella, vibrio parahaemolyticus and Staphylococcus aureus. There was no increase in bacterial count in irradiated frozen shrimps after two months of transportation. The results of flora analysis show that radiation does not present any hazard resulting from a shift in the microflora. The surviving microflora in both types of irradiated frozen shrimp consisted mainly of Micrococcus spp.. Besides, irradiation with dose of 3 kGy did not affect the sensory quality of the frozen shrimp within duration of transportation. It can be concluded that irradiation improves the bacteriological quality of frozen shrimp for transportation to the Netherlands

  12. 78 FR 33349 - Certain Frozen Warmwater Shrimp From Indonesia: Negative Preliminary Countervailing Duty...

    Science.gov (United States)

    2013-06-04

    ... flour of at least 95 percent purity has been applied; (3) with the entire surface of the shrimp flesh thoroughly and evenly coated with the flour; (4) with the non-shrimp content of the end product constituting.... Voluntary Respondents 6. Use of Facts Otherwise Available and Adverse Inferences 7. Analysis of Programs...

  13. 78 FR 33346 - Certain Frozen Warmwater Shrimp From the People's Republic of China: Preliminary Countervailing...

    Science.gov (United States)

    2013-06-04

    ...''' layer of rice or wheat flour of at least 95 percent purity has been applied; (3) with the entire surface of the shrimp flesh thoroughly and evenly coated with the flour; (4) with the non-shrimp content of.... Analysis of Programs 7. Calculation of the All Others Rate 8. Disclosure and Public Comment 9....

  14. 78 FR 33344 - Certain Frozen Warmwater Shrimp From India: Preliminary Countervailing Duty Determination

    Science.gov (United States)

    2013-06-04

    ... flour of at least 95 percent purity has been applied; (3) with the entire surface of the shrimp flesh thoroughly and evenly coated with the flour; (4) with the non-shrimp content of the end product constituting.... Analysis of Programs 6. Calculation of the All Others Rate 7. ITC Notification 8. Disclosure and...

  15. 78 FR 33350 - Certain Frozen Warmwater Shrimp From Thailand: Preliminary Countervailing Duty Determination

    Science.gov (United States)

    2013-06-04

    ...''' layer of rice or wheat flour of at least 95 percent purity has been applied; (3) with the entire surface of the shrimp flesh thoroughly and evenly coated with the flour; (4) with the non-shrimp content of... Investigation 3. Injury Test 4. Subsidies Valuation 5. Analysis of Programs 6. ITC Notification 7....

  16. 78 FR 33342 - Certain Frozen Warmwater Shrimp From the Socialist Republic of Vietnam: Preliminary...

    Science.gov (United States)

    2013-06-04

    ... flour of at least 95 percent purity has been applied; (3) with the entire surface of the shrimp flesh thoroughly and evenly coated with the flour; (4) with the non-shrimp content of the end product constituting... Countervailing Duty Law to Imports from the Vietnam 5. Subsidies Valuation ] 6. Analysis of Programs...

  17. 77 FR 23222 - Certain Frozen Warmwater Shrimp From the Socialist Republic of Vietnam: Final Results of...

    Science.gov (United States)

    2012-04-18

    ... limited liability company into a joint stock company, changing its name to C. P. Vietnam Livestock... Republic of Vietnam, 70 FR 5152 (February 1, 2005) (``VN Shrimp Order''). \\2\\ See VN Shrimp Order; see also... Duty Administrative Review, 75 FR 47771 (August 9, 2010). On December 13, 2011, C. P....

  18. 78 FR 50385 - Certain Frozen Warmwater Shrimp from India: Final Affirmative Countervailing Duty Determination

    Science.gov (United States)

    2013-08-19

    ... Warmwater Shrimp From India: Preliminary Countervailing Duty Determination, 78 FR 33344 (June 4, 2013... scope of this investigation does not include brine-frozen shrimp.\\4\\ We have ] addressed this request..., Thailand, and Socialist Republic of Vietnam--Final Scope Memorandum Regarding Onboard Brine-Frozen...

  19. 78 FR 50389 - Certain Frozen Warmwater Shrimp From Ecuador: Final Affirmative Countervailing Duty Determination

    Science.gov (United States)

    2013-08-19

    ...: Preliminary Negative Countervailing Duty Determination, 78 FR 33347 (June 4, 2013) (Preliminary Determination... investigation does not include ] brine-frozen shrimp.\\4\\ We have addressed this request and comments thereon in... Socialist Republic of Vietnam--Final Scope Memorandum Regarding Onboard Brine-Frozen Shrimp,'' which...

  20. Little Shrimp, Big Results: A Model of an Integrative, Cross-Curricular Activity

    Science.gov (United States)

    Ackerson, Nicole; Piser, Carol; Walka, Keith

    2010-01-01

    This integrative, cross-curricular lab engages middle school biology students in an exercise involving ecology, arthropod biology, and mathematics. Students research the anatomy and behavioral patterns of a species of brine shrimp, compare the anatomy of adult and juvenile brine shrimp, and graph and interpret results. In this article, the authors…

  1. 78 FR 50387 - Certain Frozen Warmwater Shrimp From the Socialist Republic of Vietnam: Final Affirmative...

    Science.gov (United States)

    2013-08-19

    ...: Preliminary Countervailing Duty Determination, 78 FR 33342 (June 4, 2013) (Preliminary Determination). This... investigation does not include brine-frozen shrimp.\\4\\ We have addressed this request and comments thereon in... Socialist Republic of Vietnam--Final Scope Memorandum Regarding Onboard Brine-Frozen Shrimp,'' which...

  2. 78 FR 35643 - Frozen Warmwater Shrimp from China, Ecuador, India, Indonesia, Malaysia, Thailand, and Vietnam...

    Science.gov (United States)

    2013-06-13

    ... COMMISSION Frozen Warmwater Shrimp from China, Ecuador, India, Indonesia, Malaysia, Thailand, and Vietnam..., Malaysia, Thailand, and Vietnam of frozen warmwater shrimp, provided for in subheadings 0306.17.00, 1605.21... found to be subsidized by the Governments of China, India, Malaysia, Thailand, and Vietnam and that...

  3. 78 FR 764 - Frozen Warmwater Shrimp From China, Ecuador, India, Indonesia, Malaysia, Thailand, and Vietnam...

    Science.gov (United States)

    2013-01-04

    ... COMMISSION Frozen Warmwater Shrimp From China, Ecuador, India, Indonesia, Malaysia, Thailand, and Vietnam..., Malaysia, Thailand, and Vietnam of frozen warmwater shrimp, provided for in subheadings 0306.17.00, 1605.21... subsidized by the Governments of China, Ecuador, India, Indonesia, Malaysia, Thailand, and Vietnam....

  4. Major shrimp pathogenic virus in america and their relationship with low salinity environments

    Directory of Open Access Journals (Sweden)

    Daniel Enrique Godínez Siordia

    2012-09-01

    Full Text Available Shrimp aquaculture is an expanding activity in many countries in which the health status is a determining factor for its success. In this review we present world shrimp viral agents, with emphasis in America reported virus and the influence of water salinity.

  5. Quality perceptions of stakeholders in Beninese export-oriented shrimp chain

    NARCIS (Netherlands)

    Dabade, D.S.; Besten, den H.M.W.; Azokpota, P.; Nout, M.J.R.; Hounhouigan, D.J.; Zwietering, M.H.

    2014-01-01

    In recent years, the Beninese shrimp sector has faced a ban on export to the European Union due to lack of compliance with food safety standards. The present study aimed at obtaining insight into the factors that determine shrimp quality and safety in Benin. A survey was conducted to investigate the

  6. Comparison live adult Artemia and squid meat on the growth of Penaeid shrimp Metapenaeus dobsoni (Miers)

    Digital Repository Service at National Institute of Oceanography (India)

    Nair, S.R.S.; Achuthankutty, C.T.; Royan, J.P.

    An experiment lasting 14 weeks was conducted to compare the efficiency of live adult Artemia with fresh squid meat on growth of penaeid shrimp Metapenaeus dobsoni. The shrimps were found actively feeding on live Artemia and grew 84% more than those...

  7. Effect of gamma-irradiation on frozen shrimps for decontamination of pathogenic bacteria

    International Nuclear Information System (INIS)

    Twelve samples of imported frozen shrimps were used in this study. The total aerobic bacteria were at 2 x 104 to 6 x 106 per gram. A few of Vibrio parahaemolyticus, V. mimicus, V. alginolyticus, V. vulnificus, V. fluvialis and Listeria monocytogenes were isolated from many samples. However, Salmonella was not detected in any of the samples. After exposure to 4 - 5 kGy of gamma-rays, the total aerobic bacteria in frozen shrimps were reduced by approximately 2-3 log cycles. The dose necessary to reduce the vibrio isolates and Aeromonas hydrophila at a level of below 10-4 per gram was about 3 kGy in frozen shrimps, whereas about 3.5 kGy was required for L. monocytogenes and Salmonella typhimurium. In this study, unpleasant off-odor was clearly detected in the non-frozen shrimps irradiated at 2.5 kGy. On the other hand, off-odor was negligible in the frozen product below 5 kGy irradiation. No remarkable changes of peroxide values were obtained up to 9 kGy of irradiation in the frozen shrimps. However peroxide values of non-frozen shrimps were clearly increased even irradiated at 4 kGy. Trimethylamine content was not changed at doses below 10 kGy in both of frozen and non-frozen shrimps. Shelf-life of defrosted shrimps were extended ca. 2 times under non-frozen market conditions. (author)

  8. Detetermination of hydrocarbons by GC-MS for identification of irradiated shrimp

    International Nuclear Information System (INIS)

    The shrimp were irradiated by 60Co γ rays at the dose of 1, 3, 5, and 7 kGy. The shrimp was firstly Soxhlet extracted and then purification of the shrimp oil was carried out by solid-phase extraction column to remove the fat and pigment. The hydrocarbons contained in the shrimp were determined by GC-MS among irradiated and unirradiated samples. The results show that C17:1 and C16:1 produces were the new types of hydrocarbons in irradiated shrimp with recovery of 78.2% and 88.06%, respectively. Content of C17:1 and C16:1 increased with the increase of the absorbed dose. (authors)

  9. Scanning electron microscope observations of brine shrimp larvae from space shuttle experiments

    Science.gov (United States)

    DeBell, L.; Paulsen, A.; Spooner, B.

    1992-01-01

    Brine shrimp are encysted as gastrula stage embryos, and may remain dehydrated and encysted for years without compromising their viability. This aspect of brine shrimp biology is desirable for studying development of animals during space shuttle flight, as cysts placed aboard a spacecraft may be rehydrated at the convenience of an astronaut, guaranteeing that subsequent brine shrimp development occurs only on orbit and not on the pad during launch delays. Brine shrimp cysts placed in 5 ml syringes were rehydrated with salt water and hatched during a 9 day space shuttle mission. Subsequent larvae developed to the 8th larval stage in the sealed syringes. We studied the morphogenesis of the brine shrimp larvae and found the larvae from the space shuttle experiments similar in rate of growth and extent of development, to larvae grown in sealed syringes on the ground. Extensive differentiation and development of embryos and larvae can occur in a microgravity environment.

  10. A multi-biomarker approach to assess the impact of farming systems on black tiger shrimp (Penaeus monodon).

    Science.gov (United States)

    Tu, Huynh Thi; Silvestre, Frederic; Wang, Neil; Thome, Jean-Pierre; Phuong, Nguyen Thanh; Kestemont, Patrick

    2010-11-01

    This study examined the advantages of the use of biomarkers as an early warning system by applying it to different shrimp farming systems in Soctrang and Camau provinces, main shrimp producers in Mekong River Delta, Vietnam. Shrimp were collected at 15 different farms divided into four different farming systems: three farms were converted from originally rice paddies into intensive shrimp farming systems (IS1, IS2, IS3); three farms were rice-shrimp integrated farming systems (RS4, RS5, RS6); three farms were intensive farming systems (IS7, IS8, IS9); six farms were extensive shrimp farming systems (From ES1 to ES6). Lipid peroxidation (LPO) and total glutathione (GSH) were measured as well as catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST) and acetylcholinesterase activities (ACHE). Organ specificity was observed between gills and hepatopancreas with generally higher activity of GST in gills (GSTG) whereas the contrary was observed for LPO level in gills (LPOG). Hierarchical clustering and principal component analysis clearly indicated that shrimp reared in extensive culture system formed a distinct group from those reared in intensive or rice-shrimp integrated systems. CAT in gills (CATG), GPX in gills (GPXG) and hepatopancreas (GPXHP) and ACHE in muscle (ACHEM) of shrimp collected in extensive farms showed a general higher level than those in intensively farmed shrimp. On the contrary, we observed clear high levels of GSTG and GST in hepatopancreas (GSTHP) and LPOG and hepatopancreas (LPOHP) of shrimp sampled in intensive and rice-shrimp integrated systems. Thus, we propose that LPO and CAT, GPX, GST and ACHE can be used as a set of biomarkers for the assessment of health condition and can discriminate between shrimp cultivated in different farming systems. These findings provide the usefulness of integrating a set of biomarkers to define the health status of shrimp in different shrimp culture systems. PMID:20943254

  11. Genotyping of White Spot Syndrome Virus in Chinese Cultured Shrimp during 1998-1999

    Institute of Scientific and Technical Information of China (English)

    Yan-wei Tan; Zheng-li Shi

    2011-01-01

    Recent studies showed that white spot syndrome virus(WSSV)isolates from different geographic locations share a high genetic similarity except the variable regions in ORF23/24 and ORF14/15,and variable number of tandem repeats(VNTR)within ORF94.In this study,genotyping was performed according to these three variable regions among WSSV isolates collected during 1998/1999 from Southern China.These WSSV isolates contain a deletion of 1168,5657,5898,9316 and 11093 bp,respectively in the variable region ORF23/24compared with WSSV-TW,and a deletion of 4749 or 5622 bp in the variable region ORF14/15 relative to TH-96-II.Four types of repeat units(RUs)(6,8,9 and 13 RUs)in ORF94 were detected in these isolates,with the shortest 6 RUs as the most prevalent type.Our results provide important information for a better understanding of the spatio-temporal transmission mode and the WSSV genetic evolution lineage.

  12. Effects of gamma irradiation on chemical, microbial quality and shelf life of shrimp

    International Nuclear Information System (INIS)

    In the present study the combined effect of gamma irradiation (1, 3 and 5 kGy) and storage at two temperatures: refrigeration (+4 °C) and frozen (−18 °C), on the shelf-life extension of fresh shrimp meat was investigated. The study was based on microbiological and physicochemical changes occuring in the shrimp samples. Total volatile base nitrogen values and trimethylamine values for irradiated shrimp samples were significantly lower than non-irradiated samples at both storage temperatures, and the rate of decrease was more pronounced in samples irradiated at the higher dose (p<0.05). Thiobarbituric acid values for irradiated shrimp samples were significantly higher than non-irradiated samples at both storage temperatures (p<0.05). pH values of shrimp samples were affected significantly by both irradiating dose and storage temperatures (p<0.05). Microbial counts for non-irradiated shrimp samples were higher than the respective irradiated samples at both storage temperatures (p<0.05). The results revealed that irradiation at high dose (5 kGy) might enhance lipid oxidation, although the growth of microorganisms and protein oxidation was inhibited. - Highlights: ► We examine the combined effect of irradiation and storage low temperatures on shrimp. ► Irradiation and low temperature storage reduce the bacterial growth in shrimp. ► Irradiation with frozen storage extend the shelf-life of shrimp meat to about 90 days. ► The combined application stabilize the chemical characteristics of shrimp meat

  13. Bioturbating shrimp alter the structure and diversity of bacterial communities in coastal marine sediments.

    Science.gov (United States)

    Laverock, Bonnie; Smith, Cindy J; Tait, Karen; Osborn, A Mark; Widdicombe, Steve; Gilbert, Jack A

    2010-12-01

    Bioturbation is a key process in coastal sediments, influencing microbially driven cycling of nutrients as well as the physical characteristics of the sediment. However, little is known about the distribution, diversity and function of the microbial communities that inhabit the burrows of infaunal macroorganisms. In this study, terminal-restriction fragment length polymorphism analysis was used to investigate variation in the structure of bacterial communities in sediment bioturbated by the burrowing shrimp Upogebia deltaura or Callianassa subterranea. Analyses of 229 sediment samples revealed significant differences between bacterial communities inhabiting shrimp burrows and those inhabiting ambient surface and subsurface sediments. Bacterial communities in burrows from both shrimp species were more similar to those in surface-ambient than subsurface-ambient sediment (R=0.258, P<0.001). The presence of shrimp was also associated with changes in bacterial community structure in surrounding surface sediment, when compared with sediments uninhabited by shrimp. Bacterial community structure varied with burrow depth, and also between individual burrows, suggesting that the shrimp's burrow construction, irrigation and maintenance behaviour affect the distribution of bacteria within shrimp burrows. Subsequent sequence analysis of bacterial 16S rRNA genes from surface sediments revealed differences in the relative abundance of bacterial taxa between shrimp-inhabited and uninhabited sediments; shrimp-inhabited sediment contained a higher proportion of proteobacterial sequences, including in particular a twofold increase in Gammaproteobacteria. Chao1 and ACE diversity estimates showed that taxon richness within surface bacterial communities in shrimp-inhabited sediment was at least threefold higher than that in uninhabited sediment. This study shows that bioturbation can result in significant structural and compositional changes in sediment bacterial communities, increasing

  14. Immune response and disease resistance of shrimp fed biofloc grown on different carbon sources.

    Science.gov (United States)

    Ekasari, Julie; Hanif Azhar, Muhammad; Surawidjaja, Enang H; Nuryati, Sri; De Schryver, Peter; Bossier, Peter

    2014-12-01

    The objective of this study was to document the immunological effects of growing shrimp in biofloc systems. The experiment consisted of four types of biofloc systems in which bioflocs were produced by daily supplementation of four different carbon sources, i.e. molasses, tapioca, tapioca-by-product, and rice bran, at an estimated C/N ratio of 15 and a control system without any organic carbon addition. Each biofloc system was stocked with Pacific white shrimp (Litopenaeus vannamei) juveniles that were reared for 49 days. The use of tapioca-by-product resulted in a higher survival (93%) of the shrimp as compared to the other carbon sources and the control. The highest yield and protein assimilation was observed when tapioca was used as the carbon source. After 49 days, phenoloxidase (PO) activity of the shrimp grown in all biofloc systems was higher than that of the shrimp from the control system. Following a challenge test by injection with infectious myonecrosis virus (IMNV), the levels of PO and respiratory burst (RB) activity in the shrimp of all biofloc treatments were higher than that of the challenged shrimp from the control treatment. An increased immunity was also suggested by the survival of the challenged shrimp from the experimental biofloc groups that was significantly higher as compared to the challenged shrimp from the control treatment, regardless of the organic carbon source used to grow the bioflocs. Overall, this study demonstrated that the application of biofloc technology may contribute to the robustness of cultured shrimp by immunostimulation and that this effect is independent of the type of carbon source used to grow the flocs. PMID:25218685

  15. Management of Chinese restaurant

    OpenAIRE

    Cui , Longbo

    2009-01-01

    With Chinese economy developing rapidly, the Chinese restaurant is under the spotlight, but the management of Chinese restaurant is weak at the moment, especially on the service management, which is an important part of service management in the Chinese restaurant. On the other hand, the managers of Chinese restaurant should pay more attention on the service management for instance brand, service innovation. Service management is core and essential concept for every service company recently, ...

  16. Polysaccharides as Alternative Moisture Retention Agents for Shrimp.

    Science.gov (United States)

    Torti, Michael J; Sims, Charles A; Adams, Charles M; Sarnoski, Paul J

    2016-03-01

    Phosphates are used as moisture retention agents (MRAs) by the shrimp industry. Although they are effective, phosphates are expensive, need to be listed on a food label, and overuse can often lead to a higher product cost for consumers. Polysaccharides were researched as alternative MRAs. Polysaccharides are usually inexpensive, are considered natural, and can have nutritional benefits. Research was conducted to determine whether polysaccharides yielded similar functional impacts as phosphates. Treatments included a 0.5% fibercolloid solution isolated from citrus peel, an 8% pectin solution, a 0.5% xanthan gum (XG) solution, a 1% carboxymethyl cellulose solution, and conventionally used 4% sodium tripolyphosphate (STP). Experimental treatments were compared to a distilled water control to gauge effectiveness. Freezing, boiling, and oven drying studies were performed to determine how moisture retention in shrimp differed using these different treatments. Water activity was measured to determine any potential differences in shelf life. Solution uptake was also determined to understand how well the treatments enhanced water binding. For moisture loss by freezing, 4% STP and the 0.5% fibercolloid solution functioned the best. The 4% STP treated shrimp lost the least amount of moisture during boiling. The 0.5% fibercolloid and 0.5% XG treatment outperformed phosphates in respect to moisture uptake ability. None of the treatments had a major effect on water activity. All treatments were rated similar in consumer sensory acceptability tests except for pectin, which was rated lower by the sensory panel. Overall, polysaccharides were found to be viable alternatives to phosphates. PMID:26849189

  17. Isotopic incorporation rates and discrimination factors in mantis shrimp crustaceans.

    Directory of Open Access Journals (Sweden)

    Maya S deVries

    Full Text Available Stable isotope analysis has provided insights into the trophic ecology of a wide diversity of animals. Knowledge about isotopic incorporation rates and isotopic discrimination between the consumer and its diet for different tissue types is essential for interpreting stable isotope data, but these parameters remain understudied in many animal taxa and particularly in aquatic invertebrates. We performed a 292-day diet shift experiment on 92 individuals of the predatory mantis shrimp, Neogonodactylus bredini, to quantify carbon and nitrogen incorporation rates and isotope discrimination factors in muscle and hemolymph tissues. Average isotopic discrimination factors between mantis shrimp muscle and the new diet were 3.0 ± 0.6 ‰ and 0.9 ± 0.3 ‰ for carbon and nitrogen, respectively, which is contrary to what is seen in many other animals (e.g. C and N discrimination is generally 0-1 ‰ and 3-4 ‰, respectively. Surprisingly, the average residence time of nitrogen in hemolymph (28.9 ± 8.3 days was over 8 times longer than that of carbon (3.4 ± 1.4 days. In muscle, the average residence times of carbon and nitrogen were of the same magnitude (89.3 ± 44.4 and 72.8 ± 18.8 days, respectively. We compared the mantis shrimps' incorporation rates, along with rates from four other invertebrate taxa from the literature, to those predicted by an allometric equation relating carbon incorporation rate to body mass that was developed for teleost fishes and sharks. The rate of carbon incorporation into muscle was consistent with rates predicted by this equation. Our findings provide new insight into isotopic discrimination factors and incorporation rates in invertebrates with the former showing a different trend than what is commonly observed in other animals.

  18. Pathogenesis of acute hepatopancreatic necrosis disease (AHPND) in shrimp.

    Science.gov (United States)

    Lai, Hung-Chiao; Ng, Tze Hann; Ando, Masahiro; Lee, Chung-Te; Chen, I-Tung; Chuang, Jie-Cheng; Mavichak, Rapeepat; Chang, Sheng-Hsiung; Yeh, Mi-De; Chiang, Yi-An; Takeyama, Haruko; Hamaguchi, Hiro-o; Lo, Chu-Fang; Aoki, Takashi; Wang, Han-Ching

    2015-12-01

    Acute hepatopancreatic necrosis disease (AHPND), also called early mortality syndrome (EMS), is a recently emergent shrimp bacterial disease that has resulted in substantial economic losses since 2009. AHPND is known to be caused by strains of Vibrio parahaemolyticus that contain a unique virulence plasmid, but the pathology of the disease is still unclear. In this study, we show that AHPND-causing strains of V. parahaemolyticus secrete the plasmid-encoded binary toxin PirAB(vp) into the culture medium. We further determined that, after shrimp were challenged with AHPND-causing bacteria, the bacteria initially colonized the stomach, where they started to produce PirAB(vp) toxin. At the same early time point (6 hpi), PirB(vp) toxin, but not PirA(vp) toxin, was detected in the hepatopancreas, and the characteristic histopathological signs of AHPND, including sloughing of the epithelial cells of the hepatopancreatic tubules, were also seen. Although some previous studies have found that both components of the binary PirAB(vp) toxin are necessary to induce a toxic effect, our present results are consistent with other studies which have suggested that PirB(vp) alone may be sufficient to cause cellular damage. At later time points, the bacteria and PirA(vp) and PirB(vp) toxins were all detected in the hepatopancreas. We also show that Raman spectroscopy "Whole organism fingerprints" were unable to distinguish between AHPND-causing and non-AHPND causing strains. Lastly, by using minimum inhibitory concentrations, we found that both virulent and non-virulent V. parahaemolyticus strains were resistant to several antibiotics, suggesting that the use of antibiotics in shrimp culture should be more strictly regulated. PMID:26549178

  19. Floc contribution on spawning performance of blue shrimp Litopenaeus stylirostris

    OpenAIRE

    Emerenciano, Mauricio; Cuzon, Gerard; Goguenheim, Jean; Gaxiola, Gabriela

    2012-01-01

    The floc system has been successfully applied for shrimp grow-out, but little is known about floc contribution on reproductive performance. In a 30-day trial, spawning performance of broodstock was evaluated in floc and earthen ponds. Floc spawners achieved better results (P < 0.05) compared with pond spawners in terms of number of spawns per ablated female (2.9 vs. 1.3), spawns per spawning female (3.6 vs. 2.4) and number of consecutive maturation (average of 3.6 times compared with 2.5 time...

  20. Combined electrohydrodynamic (EHD) and vacuum freeze drying of shrimp

    International Nuclear Information System (INIS)

    To improve the drying qualities of shrimp, a combination of electrohydrodynamic (EHD) and vacuum freeze drying (FD) is examined. The drying rate, the shrinkage, the rehydration ratio, and the sensory properties including the color and trimness of the dried products under different drying methods (including combination drying of EHD and FD, EHD drying and FD drying) are measured. Compared with FD and EHD drying alone, the combined process consumes less drying time, and the product processed by combined drying displays lower shrinkage, higher rehydration rate and better sensory qualities.

  1. Kinetics of Demineralization of Shrimp Shell Using Lactic Acid

    Directory of Open Access Journals (Sweden)

    Alewo Opuada AMEH

    2014-02-01

    Full Text Available Shrimp exoskeleton was demineralized using lactic acid and the kinetics of the demineralisation was studied. The residual concentration of calcium after acid treatment was used as a measure of the degree of demineralisation. Kinetic data was obtained using five acid concentrations (0.2, 0.4, 0.6, 0.8 and 1M and the obtained kinetic data was fitted to the shrinking core model. For all concentrations, the best predictive model was determined to be ash layer diffusion controlled mechanism.

  2. Northern Shrimp (Pandalus borealis) on Flemish Cap Surveys 2014

    OpenAIRE

    Casas-Sánchez, J.M. (José Miguel)

    2014-01-01

    A stratified random bottom trawl survey on Flemish Cap was carried out from June 25th to July 23th 2014. The area surveyed was extended up to depths of 800 fathoms (1450 meters) following the same procedures as in previous years. This year a total of 181 valid hauls were made by the vessel R/V Vizconde de Eza with the usual survey gear (Lofoten), 120 up to 730 meters depth. The surveyed area has properly prospected the 32 strata planned. The general indexes for shrimp were estimated taken int...

  3. Northern Shrimp (Pandalus borealis) on Flemish Cap Surveys 2015

    OpenAIRE

    Casas-Sánchez, J.M. (José Miguel)

    2015-01-01

    A stratified random bottom trawl survey on Flemish Cap was carried out from June 21th to July 26th 2015. The area surveyed was extended up to depths of 800 fathoms (1450 meters) following the same procedures as in previous years. This year a total of 181 valid hauls were made by the vessel R/V Vizconde de Eza with the usual survey gear (Lofoten), 120 up to 730 meters depth. The surveyed area has properly prospected the 32 strata planned. The general indexes for shrimp were estimated taken int...

  4. Northern Shrimp (Pandalus borealis) on Flemish Cap Surveys 2012

    OpenAIRE

    Casas-Sánchez, J.M. (José Miguel)

    2012-01-01

    A stratified random bottom trawl survey on Flemish Cap was carried out from June 26th to July 24th 2012. The area surveyed was extended up to depths of 800 fathoms (1450 meters) following the same procedures as in previous years. This year a total of 174 valid hauls were made by the vessel R/V Vizconde de Eza with the usual survey gear (Lofoten), 118 up to 730 meters depth. The surveyed area has properly prospected the 32 strata planned. The general indexes for shrimp were estimated taken int...

  5. Northern Shrimp (Pandalus borealis) on Flemish Cap Surveys 2013

    OpenAIRE

    Casas-Sánchez, J.M. (José Miguel)

    2013-01-01

    A stratified random bottom trawl survey on Flemish Cap was carried out from June 26th to July 23th 2013. The area surveyed was extended up to depths of 800 fathoms (1450 meters) following the same procedures as in previous years. This year a total of 181 valid hauls were made by the vessel R/V Vizconde de Eza with the usual survey gear (Lofoten), 120 up to 730 meters depth. The surveyed area has properly prospected the 32 strata planned. The general indexes for shrimp were estimated taken int...

  6. [Exploitation of the shrimp trawl fishery in the period 1991-1999 at the Gulf of Nicoya, Costa Rica].

    Science.gov (United States)

    Tabash Blanco, Farid A

    2007-03-01

    In Costa Rica, the Gulf of Nicoya shrimp fishery originated in 1952 and represented one of the most important economic activities in the region. Nevertheless, overfishery reduced the captured volumes to levels that prevent this commercial activity. I analyzed official fishery statistics between 1991 and 1999. These species involved are: two species of white shrimp, (Litopenaeus occidentalis and L. stylirostris), the "titi" shrimp (Xiphopenaeus riveti), the brown shrimp (Farfantepenaeus californiensis), the "pinki" shrimp (F. brevirostris) and the "fidel" shrimp (Solenocera agassizi). All the species reached the Maximum Sustainable Yield in the decades of 1970 and 1980 and are now found at over-exploitation levels. I recommend that this shrimp trawl fishery be completely closed down. PMID:18457129

  7. A multi-biomarker approach to assess the impact of farming systems on black tiger shrimp (Penaeus monodon)

    OpenAIRE

    Thi Tu, H.; SILVESTRE, F.; Wang, N; Thomé, J.-P.; Thanh Phuong, N.; Kestemont, P

    2010-01-01

    This study examined the advantages of the use of biomarkers as an early warning system by applying it to different shrimp farming systems in Soctrang and Camau provinces, main shrimp producers in Mekong River Delta, Vietnam. Shrimp were collected at 15 different farms divided into four different farming systems: three farms were converted from originally rice paddies into intensive shrimp farming systems (IS1, IS2, IS3); three farms were rice–shrimp integrated farming systems (RS4, RS5, RS6);...

  8. Effects of process parameters on quality changes of shrimp during drying in a jet-spouted bed dryer.

    Science.gov (United States)

    Niamnuy, C; Devahastin, S; Soponronnarit, S

    2007-11-01

    The objective of the present study was to investigate the effects of various parameters, that is, concentration of salt solution (2%, 3%, 4%[w/v]), boiling time (3, 5, 7 min), drying air temperature (80, 100, 120 degrees C), and size of shrimp, on the kinetics of drying and various quality attributes of shrimp, namely, shrinkage, rehydration ability, texture, colors, and microstructure, during drying in a jet-spouted bed dryer. In addition, the effects of these processing parameters on the sensory attributes of dried shrimp were also investigated. Small shrimp (350 to 360 shrimp/kg) and large shrimp (150 to 160 shrimp/kg) were boiled and then dried until their moisture content was around 25% (d.b.). It was found that the degree of color changes, toughness, and shrinkage of shrimp increased while the rehydration ability decreased with an increase in the concentration of salt solution and boiling time. Size of shrimp and drying temperature significantly affected all quality attributes of dried shrimp. The conditions that gave the highest hedonic scores of sensory evaluation for small dried shrimp are the concentration of salt solution of 2% (w/v), boiling time of 7 min, and drying air temperature of 120 degrees C. On the other hand, the conditions that gave the highest hedonic scores of sensory evaluation for large dried shrimp are the concentration of salt solution of 4% (w/v), boiling time of 7 min, and drying air temperature of 100 degrees C. The quality attributes of dried shrimp measured by instruments correlated well with the sensory attributes, especially the color of dried shrimp. PMID:18034725

  9. Current status of shrimp farming in the southwestern coast (Manroe Island, Kollam of India

    Directory of Open Access Journals (Sweden)

    C. Shakir

    2010-10-01

    Full Text Available A survey on 58 shrimp farms (42 operational and 16 non-operational was conducted within the Manroe island over 12 weeks. The black tiger shrimp, Penaeus monodon was the main species cultured. Presently, small-scale farmers carry out 90% shrimp aquaculture in Manroe Island. The farming is characterized by modified extensive and semi-intensive production systems, largely dependent on small ponds of less than 1 ha. These farms account for ~90% of the total area utilized for shrimp culture. The stocking densities range between 4 to 6 post larvae /m2 and average shrimp yields after 90-120 days is 320-400 kg.ha-1. As per our survey the average production cost for shrimp per hectare is at least 1,50,000 per cycle. During the 1990s, shrimp was cash crop in the Kollam with an average production of 900 kg.ha-1. Currently, the unsustainable practices and devastating outbreaks of disease has forced the industry to shut down.

  10. Modulation of Mutagenicity of Various Mutagens by Shrimp Flesh and Skin Extracts in Salmonella Test

    Directory of Open Access Journals (Sweden)

    S. Mehrabian

    2006-01-01

    Full Text Available Many mutagens and carcinogens may act through the generation of Reactive Oxygen Species (ROS in cells that maybe related to cancer. The carotenoid astaxanthin-the red pigment for the attractive coloration in the skin and flesh of shrimp-has attracted considerable interest in recent years because of its superior antioxidative activity to most of the hydrophobic antioxidants. According to the important role of carotenoids in human health, the main carotenoid pigment in flesh and by-products of the different shrimp species from penaeidae family-Astaxanthin-was extracted and separated by thin layer chromatography using silica gel, subsequently. The identifications were confirmed by fourier transform infrared (ET-IR spectroscopy. The effect of these extracts on mutagenicity and carcinogenicity induced by Sodium Azide and potassium permanganate was investigated in bacterial assay system, i.e., the Ames test with salmonella typhimurium TA100, TA104. These strains have a certain mutation in their histidin operon, which are related to the external histidin source. Such a mutation can be reversed through contact with a diagnostic mutagen, but in shrimp extracts prevented from effect of this mutagen in which, after repeated test series under standardized condition, the anticarcinogenic effect of flesh and skin of shrimp was proven and the shrimp skin showed stronger anticarcinogenic effect in comparison with shrimp flesh. Different shrimp species from penaeidae family indicated similar antricariongenic effects.

  11. The effects of Aloe vera (Aloe barbadensis) coating on the quality of shrimp during cold storage.

    Science.gov (United States)

    Soltanizadeh, Nafiseh; Mousavinejad, Mohsen S

    2015-10-01

    Green tiger shrimp (Penaeus semisulcatus) is an important aquaculture species worldwide. Its perishable nature, however, needs preservation methods to ensure its quality and shelf life. In this study, the effects of Aloe vera coating on the quality and shelf life of shrimps during cold storage were investigated. Shrimp samples were dipped in aqueous solutions containing 25%, 50%, 75%, and 100% Aloe vera gel before storage at 4 °C for 7 days. Drip loss, pH, TBA, TVB-N, and texture of both the control and treated shrimp samples were analyzed periodically. There were significant differences between coated shrimps and the control group in all parameters evaluated. Aloe vera at 75% and 100% concentrations was able to prevent lipid oxidation and drip loss properly; however, coatings containing 25% Aloe vera did not have the desired effects on these characteristics. Shrimps coated with higher concentrations of Aloe vera had better textural properties during cold storage. Results also indicated the positive effects of Aloe vera coating on the sensory quality of shrimp. PMID:26396412

  12. Contests with deadly weapons: telson sparring in mantis shrimp (Stomatopoda).

    Science.gov (United States)

    Green, P A; Patek, S N

    2015-09-01

    Mantis shrimp strike with extreme impact forces that are deadly to prey. They also strike conspecifics during territorial contests, yet theoretical and empirical findings in aggressive behaviour research suggest competitors should resolve conflicts using signals before escalating to dangerous combat. We tested how Neogonodactylus bredini uses two ritualized behaviours to resolve size-matched contests: meral spread visual displays and telson (tailplate) strikes. We predicted that (i) most contests would be resolved by meral spreads, (ii) meral spreads would reliably signal strike force and (iii) strike force would predict contest success. The results were unexpected for each prediction. Contests were not resolved by meral spreads, instead escalating to striking in 33 of 34 experiments. The size of meral spread components did not strongly correlate with strike force. Strike force did not predict contest success; instead, winners delivered more strikes. Size-matched N. bredini avoid deadly combat not by visual displays, but by ritualistically and repeatedly striking each other's telsons until the loser retreats. We term this behaviour 'telson sparring', analogous to sparring in other weapon systems. We present an alternative framework for mantis shrimp contests in which the fight itself is the signal, serving as a non-lethal indicator of aggressive persistence or endurance. PMID:26399976

  13. Control of social monogamy through aggression in a hermaphroditic shrimp

    Directory of Open Access Journals (Sweden)

    Wong Janine WY

    2011-11-01

    Full Text Available Abstract Introduction Sex allocation theory predicts that in small mating groups simultaneous hermaphroditism is the optimal form of gender expression. Under these conditions, male allocation is predicted to be very low and overall per-capita reproductive output maximal. This is particularly true for individuals that live in pairs, but monogamy is highly susceptible to cheating by both partners. However, certain conditions favour social monogamy in hermaphrodites. This study addresses the influence of group size on group stability and moulting cycles in singles, pairs, triplets and quartets of the socially monogamous shrimp Lysmata amboinensis, a protandric simultaneous hermaphrodite. Results The effect of group size was very strong: Exactly one individual in each triplet and exactly two individuals in each quartet were killed in aggressive interactions, resulting in group sizes of two individuals. All killed individuals had just moulted. No mortality occurred in single and pair treatments. The number of moults in the surviving shrimp increased significantly after changing from triplets and quartets to pairs. Conclusion Social monogamy in L. amboinensis is reinforced by aggressive expulsion of supernumerous individuals. We suggest that the high risk of mortality in triplets and quartets results in suppression of moulting in groups larger than two individuals and that the feeding ecology of L. amboinensis favours social monogamy.

  14. Structure of Penaeus stylirostris Densovirus, a Shrimp Pathogen

    Energy Technology Data Exchange (ETDEWEB)

    Kaufmann, Bärbel; Bowman, Valorie D.; Li, Yi; Szelei, Jozsef; Waddell, Peter J.; Tijssen, Peter; Rossmann, Michael G. (INRS); (Purdue)

    2010-11-16

    Penaeus stylirostris densovirus (PstDNV), a pathogen of penaeid shrimp, causes significant damage to farmed and wild shrimp populations. In contrast to other parvoviruses, PstDNV probably has only one type of capsid protein that lacks the phospholipase A2 activity that has been implicated as a requirement during parvoviral host cell infection. The structure of recombinant virus-like particles, composed of 60 copies of the 37.5-kDa coat protein, the smallest parvoviral capsid protein reported thus far, was determined to 2.5-{angstrom} resolution by X-ray crystallography. The structure represents the first near-atomic resolution structure within the genus Brevidensovirus. The capsid protein has a {beta}-barrel 'jelly roll' motif similar to that found in many icosahedral viruses, including other parvoviruses. The N-terminal portion of the PstDNV coat protein adopts a 'domain-swapped' conformation relative to its twofold-related neighbor similar to the insect parvovirus Galleria mellonella densovirus (GmDNV) but in stark contrast to vertebrate parvoviruses. However, most of the surface loops have little structural resemblance to any of the known parvoviral capsid proteins.

  15. Prevalence and Antimicrobial Resistance of Vibrio spp. in Retail and Farm Shrimps in Ecuador.

    Science.gov (United States)

    Sperling, L; Alter, T; Huehn, S

    2015-11-01

    The aim of this study was to investigate the prevalence of Vibrio spp. in shrimp at retail and in shrimp farms in Ecuador and to determine the antimicrobial agent resistance patterns of farm isolates. The presence of genes linked to early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) also was evaluated. Vibrio spp. were isolated from retail shrimps in Cuenca, Ecuador, and farm shrimps originating from provinces El Oro and Guayas, Ecuador. A total of 229 shrimp samples were collected, of which 71 originated from retail markets in Cuenca and 158 came from shrimp farms. Overall, 219 (95.6%) samples tested positive for Vibrio spp. Vibrio parahaemolyticus (80.8%) was the most common species detected, followed by Vibrio alginolyticus (50.2%), Vibrio cholerae (11.3%), and Vibrio vulnificus (3.5%). None of the V. parahaemolyticus isolates carried the virulence-associated tdh and trh genes. In V. parahaemolyticus shrimp farm isolates, high resistance was found to ampicillin (92.2%), and intermediate resistance was found to tetracycline (51.3%) and amikacin (22.1%). Of the V. parahaemolyticus strains, 68 were resistant to at least three antimicrobial agents, and 2 were resistant to seven antimicrobial agents simultaneously. Up to 18 resistant isolates were found for V. alginolyticus, whereas V. vulnificus and V. cholerae isolates were more susceptible. None of the V. parahaemolyticus isolates carried the EMS-AHPND plasmid. The results of this study revealed the ubiquitous occurrence of Vibrio spp. in shrimps at retail and on shrimp farms in Ecuador. PMID:26555534

  16. Drying Strategy of Shrimp using Hot Air Convection and Hybrid Infrared Radiation/Hot Air Convection

    Directory of Open Access Journals (Sweden)

    Supawan TIRAWANICHAKUL

    2008-01-01

    Full Text Available The main objective of the research was to study the effect of drying temperatures using infrared irradiation and electric heating convection on dehydration and was to investigate the effect of drying conditions on the quality of the shrimp. Two sizes of fresh shrimp (100 shrimp/kg and 200 shrimp/kg with initial moisture content of 270 - 350 % dry-basis were dried under various conditions while the final moisture content of dried shrimp was in ranges between 20 and 25 % dry-basis. Hot air flow rates of 1.0 -   1.2 m/s, drying temperatures of 40 - 90 °C and infrared intensities of 1,785.7 - 3,571.4 W/m2 were used in these experiments. The experimental results showed that the rate of moisture content transfer of both sizes of shrimps decreased exponentially with drying time while increasing drying temperature significantly affected to the drying kinetics and quality of the shrimps. Effective diffusion coefficients of both shrimps were determined by a diffusion model forming a finite cylindrical shape was in order of 10-7 m2/s and this effective diffusion coefficient value was relatively dependent on the drying temperature compared to the initial moisture content. The quality analysis of dried shrimp using an infrared source and electric heating source found that the redness value (Hunter a-value of dried samples using hybrid infrared radiation and electric heating had a higher colour uniformity than other drying methods. Additionally, shrinkage and rehydration properties were insignificantly different for all drying strategies (p < 0.05 and drying using infrared radiation had higher drying rates compared to electric heat convection, corresponding to relatively low drying times.

  17. Northern Shrimp (Pandalus borealis) Recruitment in West Greenland Waters

    DEFF Research Database (Denmark)

    Pedersen, S. A.; Storm, L. M.

    2002-01-01

    Lipid class and fatty acid compositions were determined in shrimp larvae (Pandalus borealis and P. montagui) collected along transects across banks on the West Greenland shelf in June 1999, May and July 2000. The lipid class contents were investigated as indices of larval shrimp lipid condition and...... food type. Fatty acid compositions were investigated for lipid biomarkers to establish trophic relationships between larval shrimp and potential prey. Phospholipids were the dominant lipid class in all six pelagic development stages of larval P. borealis and P. montagui, accounting for 80 to 92% of the...

  18. Antimicrobial effect of dietary oregano essential oil against Vibrio bacteria in shrimps

    Directory of Open Access Journals (Sweden)

    Gracia-Valenzuela M.H.

    2014-01-01

    Full Text Available The effect of dietary oregano essential oils on the growth of Vibrio bacteria in shrimps was evaluated. Shrimps were fed: (i food with oregano oil with a high level of thymol; (ii food with oregano oil with a high level of carvacrol, and (iii food without oregano oil (the control. The animals were infected by three species of Vibrio (vulnificus, parahaemolyticus and cholerae. The microbial counts of Vibrio species were significantly lower (p <0.05 in tissues from animals whose food was supplemented with oregano oil. We concluded that dietary supplementation of shrimps with oregano oil provides antimicrobial activity into the body of the penaeids.

  19. Experimental design and estimation of growth rate distributions in size-structured shrimp populations

    International Nuclear Information System (INIS)

    We discuss inverse problem results for problems involving the estimation of probability distributions using aggregate data for growth in populations. We begin with a mathematical model describing variability in the early growth process of size-structured shrimp populations and discuss a computational methodology for the design of experiments to validate the model and estimate the growth-rate distributions in shrimp populations. Parameter-estimation findings using experimental data from experiments so designed for shrimp populations cultivated at Advanced BioNutrition Corporation are presented, illustrating the usefulness of mathematical and statistical modeling in understanding the uncertainty in the growth dynamics of such populations

  20. Effect of farm made feeds on polyculture of shrimp (Penaeus monodon) and three brackishwater finfish species

    OpenAIRE

    Shofiquzzoha, A.F.M; Islam, M.L; Ahmed, S.U.

    2003-01-01

    A study was conducted to evaluate the effects of feed made from locally available ingredients on polyculture of shrimp and three brackishwater finfish species. Hatchery produced post-larvae (PL) of shrimp Penaeus monodon (0.005g) were stocked at the rate of 15,000 PLs/ha. Brackishwater finfish species Liza parsia, Mugil cephalus and Rhinomugil corsula of 0.63-1.4lg collected from local rivers were stocked at the rate 8,000, 1,000 and 2,000/ha, respectively in four treatments. Shrimp and fi...

  1. Evaluation of the bioactivities of some Myanmar medicinal plants using brine shrimp (Artemia salina) toxicity test

    International Nuclear Information System (INIS)

    For a variety of toxic substances, brine shrimp larvae (Artemia salina) are usually used as a simple bioassay method and it is also applied for natural product research. The brine shrimp larvae (nauplii) are obtained by natural hatching method from Artemia cysts. By using the larvae, the results from these experiments lead to the lethal dose, LD50 values of extracts of selected medicinal plants. Activities of a broad range of plant extracts are manifested as toxicity to the brine shrimp. Screening results with six plant extracts are compared with pure caffeine. This method is rapid, reliable, inexpensive and convenient. (author)

  2. A Brine Shrimp Bioassay for Measuring Toxicity and Remediation of Chemicals

    Science.gov (United States)

    Lieberman, Marya

    1999-12-01

    A bioassay using Artemia franciscana (brine shrimp) was adapted to measure the toxicity of household chemicals. One project is described in which students collect dose-response curves for seven commercial flea-killing products. Next, groups of students researched the insecticidal ingredients of the flea products. On the basis of the structures of the active ingredients, they chose remediation methods to make the flea product less toxic to brine shrimp; procedures included copper-catalyzed hydrolysis, adsorption onto activated charcoal, bleach treatment, and photodegradation. No special equipment or supplies are necessary for the bioassay other than the brine shrimp eggs, which can be obtained at any aquarium store.

  3. Influence of probiotics on the growth and digestive enzyme activity of white Pacific shrimp ( Litopenaeus vannamei)

    Science.gov (United States)

    Gómez, R. Geovanny D.; Shen, M. A.

    2008-05-01

    The influence of Bacillus probiotics on the digestive enzyme activity and the growth of Litopenaeus vannamei were determined in this study. The shrimp was treated with five percentages (1.5, 3.0, 4.5, 6.0 and 7.5) of probiotics ( Bacillus spp.) supplemented to the feed and cultured for 45d. The growth measured as the weight gain at the end of culturing was significantly ( Pprobiotic-treated shrimps than that of the control (without receiving probiotics). Activities of protease and amylase, two digestive enzymes of the midgut gland and the intestine were significantly ( Pprobiotic-treated shrimp than in the control.

  4. Happy (Chinese) New Year!

    Science.gov (United States)

    Johnson, Georgia G.

    1979-01-01

    Suggestions are made for a classroom celebration of Chinese New Year, including discussion of the Chinese calendar and customs, a short list of appropriate children's stories, and food ideas, including a recipe for fortune cookies. (SJL)

  5. Chinese restaurant syndrome

    Directory of Open Access Journals (Sweden)

    Balachandran C

    1991-01-01

    Full Text Available A 24-year-old Chinese student with history of recurrent attacks of flushing with burning and dryness of face of 4 years duration showed exacerbation of the symptoms after oral provocation with 1 mg of Chinese salt. Patient was treated with 50 mg pyridoxine daily and restriction of the Chinese salt in diet with moderate improvement.

  6. Chinese varkens in Nederland

    OpenAIRE

    Zhang, W.L.; Huiskes, J.H.

    1992-01-01

    In China zijn in totaal 300 miljoen varkens van meer dan 100 rassen. Ze worden voor verschillende soorten productie gebruikt. Sommige Chinese rassen zijn in Frankrijk, Nederland en U.S.A. geomporteerd. De vraag is of Chinese varkens nuttig kunnen zijnvoor de varkensproductie in Nederland en zo ja welke Chinese varkens

  7. Transboundary movement of shrimp viruses in crustaceans and their products: a special risk?

    Science.gov (United States)

    Jones, Brian

    2012-06-01

    Shrimp and shrimp products form the most valuable internationally traded fisheries commodity, and the volumes are huge, estimated to be about 3.6 million tonnes. However, despite the existence under the General Agreement on Tariffs and Trade, of the Sanitary and Phytosanitary Measures (SPS Agreement) and the activities of the World Organisation for Animal Health (OIE), viral shrimp epizootics have spread and continue to spread, affecting world production. Though most attention has focussed on the movement of live shrimp product, the spread of new and emerging diseases through other crustaceans and their nonviable products is of increasing concern. The risks associated with the unrestricted movement of nonviable product will be outlined and measures that can be taken to mitigate the risk are discussed. Ultimately, for crustacean diseases, the paradigm under which the OIE has operated for the past 80 years needs to change. PMID:22434004

  8. 78 FR 33347 - Certain Frozen Warmwater Shrimp From Ecuador: Preliminary Negative Countervailing Duty Determination

    Science.gov (United States)

    2013-06-04

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF COMMERCE International Trade Administration Certain Frozen Warmwater Shrimp From Ecuador: Preliminary Negative... of Commerce. SUMMARY: The Department of Commerce (``the Department'') preliminarily determines...

  9. Final Critical Habitat for Longhorn Fairy Shrimp (Branchinecta longiantenna) - Vernal Pool Species

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — These data identify, in general, the areas where final critical habitat for Longhorn fairy shrimp (Branchinecta longiantenna), a vernal pool crustacean, occur.

  10. Molecular epidemiology of Vibrio nigripulchritudo, a pathogen of cultured penaeid shrimp (Litopenaeus stylirostris) in New Caledonia.

    Science.gov (United States)

    Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; de Decker, Sophie; Saulnier, Denis; le Roux, Frédérique

    2006-11-01

    A collection of 57 isolates of Vibrio nigripulchritudo from either diseased or healthy shrimp and from shrimp farms environment was studied in order to gain a better understanding of the epidemiology of this pathogen, notably isolated from two distinct shrimp disease complexes. Molecular typing using two different techniques, arbitrarily primed PCR (AP-PCR) and multi-locus sequence typing (MLST), studied together with experimental pathology data allowed a relevant epidemiological insight into this possibly emerging pathogen. Additionally, results obtained with the two molecular typing techniques were congruent and allowed discriminating the strains associated with the "Summer Syndrome" from strains isolated from other contexts, especially the other shrimp vibriosis "Syndrome 93". These results highlight that the "Summer Syndrome" is most probably caused by an emergent clonal pathogen that therefore deserves surveillance and that AP-PCR can satisfactorily be used for that purpose. PMID:16413158

  11. Penaeoid and sergestoid shrimps from the deep scattering layer (DSL) in the Arabian Sea

    Digital Repository Service at National Institute of Oceanography (India)

    Karuppasamy, P.K.; Menon, N.G.

    Results of a preliminary study on the occurrence and distribution of seventeen species of Penaeoid and Sergestoid shrimps from the deep scattering layer (DSL) of the Indian EEZ of Arabian Sea are presented here based on the IKMT samples collected...

  12. Production of giant freshwater prawn postlarvae in penaeid prawn (shrimp) hatchery: An experience

    Digital Repository Service at National Institute of Oceanography (India)

    Achuthankutty, C.T.; Chatterji, A.; Sripada, R.A.; Desai, U.M.

    A pilot scale penaeid prawn (shrimp) hatchery was established at the National Institute of Oceanography, Goa, India in 1996 with an installed production capacity of 2 million postlarvae (PL 20) as part of the Technology Project in Mission Mode Semi...

  13. Clonal occurrence of Salmonella Weltevreden in cultured shrimp in the Mekong Delta, Vietnam

    DEFF Research Database (Denmark)

    Uddin, Gazi Md Noor; Larsen, Marianne Halberg; Barco, Lisa;

    2015-01-01

    yielding S. Agona, with no difference in Salmonella occurrence between the two production systems. Pulsed field gel electrophoresis (PFGE) of S. Weltevreden showed closely related XbaI pulse types, suggesting a clonal relationship despite the farms and shrimp samples being epidemiologically unrelated. S......This study investigated the occurrence, serovar and antimicrobial resistance of Salmonella spp. in shrimp samples from intensive and extensive farms located in three different provinces in the Mekong Delta, Vietnam. Shrimp from 11 of the 48 farms all contained S. Weltevreden, except for one farm....... Weltevreden was susceptible to most antimicrobials tested, with a few strains being resistant to florfenicol, chloramphenicol, sulfamethoxazole or trimethoprim. Future studies of the ecology of S. Weltevreden should establish if this serovar may survive better and even multiply in warm-water shrimp farm...

  14. The potential of Yucca schidigera extract to reduce the ammonia pollution from shrimp farming.

    Science.gov (United States)

    Santacruz-Reyes, Roberto A; Chien, Yew-Hu

    2012-06-01

    This study investigated the feasibility of using Yucca schidigera extract (YUPE) to reduce ammonia pollution resulting from shrimp farming. YUPE was added at ratios of 18, 36, and 72 mg L(-1) to effluent from an experimental shrimp culture system containing total ammonia nitrogen (TAN) of 0.592, 0.672, and 0.718 mg L(-1). TAN reduction increased with an increase in YUPE, and at 18 mg L(-1) reduced TAN by 71-72% and 86-87% at 6 and 24h, respectively. Shrimp feed containing YUPE at 300, 600, and 1200 mg kg(-1) was submerged, allowing the ammonia to leach out. The addition of YUPE in feed reduced TAN leaching by 50-83% compared with the control. The application of YUPE in water or feed could considerably reduce the accumulation of ammonia in waste water discharge during shrimp production, thereby providing a feasible solution to ammonia pollution in coastal environments. PMID:22440573

  15. Flour production from shrimp by-products and sensory evaluation of flour-based products

    Directory of Open Access Journals (Sweden)

    Thiago Mendes Fernandes

    2013-08-01

    Full Text Available The objective of this work was to evaluate the production of flour using by-products (cephalothorax obtained from the shrimp (Litopenaeus vannamei industry, and to perform a sensory analysis of shrimp flour-based products. Physicochemical and microbiological analyses on fresh cephalothorax and on manufactured flour were performed, as well as the determination of cholesterol content of this flour, and the sensorial evaluation of soup and pastry made with this flour. By the microbiological analyses, no pathogenic microorganism was detected in the samples. Physicochemical analyses of flour showed high levels of protein (50.05% and minerals (20.97%. Shrimp cephalothorax flour showed high levels of cholesterol. The sensory evaluation indicated a good acceptance of the products, with satisfactory acceptability index (81% for soup, and 83% for pastry, which indicates that shrimp cephalothorax in the form of flour has a potential for developing new products.

  16. THE BRINE SHRIMP (ARTEMIA SALINA) LETHALITY OF Brassica oleracea var. capitata

    OpenAIRE

    O. T. TÜZÜN, E. GÜRKAN, S. DOĞANCA, F. HIRLAK,

    2015-01-01

    This work covers up the bio-activities of the five fractions obtained from the ethanolic extract of Brassica oleracea var. capitata (Cruciferae).Key Words: Brassica oleracea var. capitata, Brine shrimp (Artemia salina)

  17. Use of a Brine Shrimp Assay to Study Herbal Teas in the Classroom.

    Science.gov (United States)

    Opler, Annette; Mizell, Rebecca; Robert, Alexander; Cervantes-Cervantes, Miguel; Kincaid, Dwight; Kennelly, Edward J.

    2002-01-01

    Introduces a brine shrimp assay to demonstrate the effects of the biological activity of herbal remedies. Describes two protocols, one using aqueous extracts and the other using methanol extracts. (Contains 21 references.) (YDS)

  18. 78 FR 50383 - Certain Frozen Warmwater Shrimp From the Republic of Indonesia: Final Negative Countervailing...

    Science.gov (United States)

    2013-08-19

    ...: Negative Preliminary Countervailing Duty Determination, 78 FR 33349 (June 4, 2013) (Preliminary... Memorandum Regarding Onboard Brine-Frozen Shrimp'' (Scope Memorandum), which is dated concurrently with and... suspend liquidation of entries of subject merchandise. \\4\\ See Preliminary Determination, 78 FR...

  19. Brine shrimp lethality and antibacterial activity of extracts from the bark of Schleichera oleosa

    Institute of Scientific and Technical Information of China (English)

    Laxman Pokhrel; Bigyan Sharma; Gan B Bajracharya

    2015-01-01

    Objective: To determine the antibacterial efficacy and brine shrimp toxicity of extracts (hexane, dichloromethane, ethyl acetate, methanol and water) obtained from the bark of Schleichera oleosa. Methods: The powdered bark sample was Soxhlet extracted sequentially in hexanes, dichloromethane, ethyl acetate, methanol and water. Antibacterial evaluation was carried out by following the agar diffusion method and amoxicillin disc was used as a reference. Slightly modified Meyer’s method was used to determine the toxicity of the extracts in brine shrimps. Results: Among the nine bacterial strains tested, the methanolic and aqueous extracts showed promising antibacterial efficacy against Serratia marcescens, Escherarichia coli, Bacillus subtilis and Micrococcus luteus. None of the extracts were found significantly toxic to brine shrimps. Conclusions: Strong antibacterial activity and low brine shrimp toxicity of methanolic and aqueous extracts can provide new antibacterial compounds.

  20. 78 FR 11221 - Frozen Warmwater Shrimp From China, Ecuador, India, Indonesia, Malaysia, Thailand, and Vietnam

    Science.gov (United States)

    2013-02-15

    ... COMMISSION Frozen Warmwater Shrimp From China, Ecuador, India, Indonesia, Malaysia, Thailand, and Vietnam... States is materially injured by reason of imports from China, Ecuador, India, Indonesia, Malaysia... the Governments of China, Ecuador, India, Indonesia, Malaysia, Thailand, and Vietnam.\\2\\ \\1\\...

  1. 78 FR 13325 - Certain Frozen Warmwater Shrimp From the People's Republic of China, Ecuador, India, Indonesia...

    Science.gov (United States)

    2013-02-27

    ..., Malaysia, Thailand, and the Socialist Republic of Vietnam: Postponement of Preliminary Determinations in... frozen warmwater shrimp from the People's Republic of China, Ecuador, India, Indonesia, Malaysia... of China,Ecuador, India, Indonesia, Malaysia, Thailand, and the Socialist Republic of...

  2. Brine shrimp lethality and antibacterial activity of extracts from the bark of Schleichera oleosa

    Directory of Open Access Journals (Sweden)

    Laxman Pokhrel

    2015-08-01

    Full Text Available Objective: To determine the antibacterial efficacy and brine shrimp toxicity of extracts (hexane, dichloromethane, ethyl acetate, methanol and water obtained from the bark of Schleichera oleosa. Methods: The powdered bark sample was Soxhlet extracted sequentially in hexanes, dichloromethane, ethyl acetate, methanol and water. Antibacterial evaluation was carried out by following the agar diffusion method and amoxicillin disc was used as a reference. Slightly modified Meyer’s method was used to determine the toxicity of the extracts in brine shrimps. Results: Among the nine bacterial strains tested, the methanolic and aqueous extracts showed promising antibacterial efficacy against Serratia marcescens, Escherarichia coli, Bacillus subtilis and Micrococcus luteus. None of the extracts were found significantly toxic to brine shrimps. Conclusions: Strong antibacterial activity and low brine shrimp toxicity of methanolic and aqueous extracts can provide new antibacterial compounds.

  3. Production dynamics of Brine Shrimp (Artemia franciscana) in the San Francisco Bay National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Abundance, stage structure and population dynamics of brine shrimp Artemia franciscana in pond M4 of the San Francisco Bay National Wildlife Refuge were documented...

  4. Assimilation and subcellular partitioning of elements by grass shrimp collected along an impact gradient

    Energy Technology Data Exchange (ETDEWEB)

    Seebaugh, David R. [Department of Biology, Graduate School and University Center, City University of New York, 365 Fifth Avenue, New York, NY 10016 (United States); Wallace, William G., E-mail: wallace@mail.csi.cuny.edu [Department of Biology, Graduate School and University Center, City University of New York, 365 Fifth Avenue, New York, NY 10016 (United States); Department of Biology, College of Staten Island, 6S-310, City University of New York, 2800 Victory Boulevard, Staten Island, NY 10314 (United States)

    2009-06-28

    Chronic exposure to polluted field conditions can impact metal bioavailability in prey and may influence metal transfer to predators. The present study investigated the assimilation of Cd, Hg and organic carbon by grass shrimp Palaemonetes pugio, collected along an impact gradient within the New York/New Jersey Harbor Estuary. Adult shrimp were collected from five Staten Island, New York study sites, fed {sup 109}Cd- or {sup 203}Hg-labeled amphipods or {sup 14}C-labeled meals and analyzed for assimilation efficiencies (AE). Subsamples of amphipods and shrimp were subjected to subcellular fractionation to isolate metal associated with a compartment presumed to contain trophically available metal (TAM) (metal associated with heat-stable proteins [HSP - e.g., metallothionein-like proteins], heat-denatured proteins [HDP - e.g., enzymes] and organelles [ORG]). TAM-{sup 109}Cd% and TAM-{sup 203}Hg% in radiolabeled amphipods were {approx}64% and {approx}73%, respectively. Gradients in AE-{sup 109}Cd% ({approx}54% to {approx}75%) and AE-{sup 203}Hg% ({approx}61% to {approx}78%) were observed for grass shrimp, with the highest values exhibited by shrimp collected from sites within the heavily polluted Arthur Kill complex. Population differences in AE-{sup 14}C% were not observed. Assimilated {sup 109}Cd% partitioned to the TAM compartment in grass shrimp varied between {approx}67% and {approx}75%. {sup 109}Cd bound to HSP in shrimp varied between {approx}15% and {approx}47%, while {sup 109}Cd associated with metal-sensitive HDP was {approx}17% to {approx}44%. Percentages of assimilated {sup 109}Cd bound to ORG were constant at {approx}10%. Assimilated {sup 203}Hg% associated with TAM in grass shrimp did not exhibit significant variation. Percentages of assimilated {sup 203}Hg bound to HDP ({approx}47%) and ORG ({approx}11%) did not vary among populations and partitioning of {sup 203}Hg to HSP was not observed. Using a simplified biokinetic model of metal accumulation from

  5. Assimilation and subcellular partitioning of elements by grass shrimp collected along an impact gradient

    International Nuclear Information System (INIS)

    Chronic exposure to polluted field conditions can impact metal bioavailability in prey and may influence metal transfer to predators. The present study investigated the assimilation of Cd, Hg and organic carbon by grass shrimp Palaemonetes pugio, collected along an impact gradient within the New York/New Jersey Harbor Estuary. Adult shrimp were collected from five Staten Island, New York study sites, fed 109Cd- or 203Hg-labeled amphipods or 14C-labeled meals and analyzed for assimilation efficiencies (AE). Subsamples of amphipods and shrimp were subjected to subcellular fractionation to isolate metal associated with a compartment presumed to contain trophically available metal (TAM) (metal associated with heat-stable proteins [HSP - e.g., metallothionein-like proteins], heat-denatured proteins [HDP - e.g., enzymes] and organelles [ORG]). TAM-109Cd% and TAM-203Hg% in radiolabeled amphipods were ∼64% and ∼73%, respectively. Gradients in AE-109Cd% (∼54% to ∼75%) and AE-203Hg% (∼61% to ∼78%) were observed for grass shrimp, with the highest values exhibited by shrimp collected from sites within the heavily polluted Arthur Kill complex. Population differences in AE-14C% were not observed. Assimilated 109Cd% partitioned to the TAM compartment in grass shrimp varied between ∼67% and ∼75%. 109Cd bound to HSP in shrimp varied between ∼15% and ∼47%, while 109Cd associated with metal-sensitive HDP was ∼17% to ∼44%. Percentages of assimilated 109Cd bound to ORG were constant at ∼10%. Assimilated 203Hg% associated with TAM in grass shrimp did not exhibit significant variation. Percentages of assimilated 203Hg bound to HDP (∼47%) and ORG (∼11%) did not vary among populations and partitioning of 203Hg to HSP was not observed. Using a simplified biokinetic model of metal accumulation from the diet, it is estimated that site-specific variability in Cd AE by shrimp and tissue Cd burdens in field-collected prey (polychaetes Nereis spp.) could potentially

  6. Physiochemical Changes and Optimization of Phosphate-Treated Shrimp (Litopenaeus vannamei ) Using Response Surface Methodology

    OpenAIRE

    Omar, Saiah Djebbour; Yang, Je-Eun; Oh, Sang-Cheol; Kim, Dae-Wook; Lee, Yang-Bong

    2016-01-01

    The objective of this study was to determine the factors responsible for the changed physiochemical properties of unpeeled shrimp treated in cold phosphate solution (2~4°C) with the intervention of 4 factors: phosphate concentration, dipping time, rotation speed, and volume of brine solution. Response surface analysis was used to characterize the effect of the phosphate treatment on shrimps by running 33 treatments for optimizing the experiment. For each treatment, phosphate amount, moisture ...

  7. A GIS based Site Suitability Analysis for Shrimp Cultivation in the Coastal Region of Bangladesh

    OpenAIRE

    Abu Syeed, Kazi

    2007-01-01

    Bangladesh geographically comprised one of the largest delta landscapes of the world. Almost 6.7% of country’stotal area (147570 sqkm.) is covered byrivers and inland water bodies. These water bodies being rich in fishproduction meet the majority of the demand of protein. Bangladesh produces world's fourth largest quantity of fishand it is collected from the inland water bodies. Though shrimps were easily available in the inland water bodies forhundreds of years but shrimp culture as an expor...

  8. Protective Efficacy of Clinacanthus nutans on Yellow-head Disease in Black Tiger Shrimp (Penaeus monodon)

    OpenAIRE

    Direkbusarakom, S.; Ruangpan, L.; Ezura, Y.; Yoshimizu, M.

    1998-01-01

    The leaves of Clinacanthus nutans Lindua, a well-known Thai traditional medicine against viral disease in human being, were used to extract antiviral substances by ethanol using soxhlet apparatus. The extract was tested for its activity against yellow-head rhabdovirus (YRV) in black tiger shrimp (Penaeus monodon). Virucidal effect was investigated by observation of mortality of the shrimp injected with the extract-treated virus. The results showed that extract of C. nutans inhibited YRV in vi...

  9. Genetic improvement strategy in small aquaculture industries : the new caledonian shrimp experience

    OpenAIRE

    Goyard, Emmanuel; Goarant, Cyrille; Brun, Pierre; Herlin, Jose; Pham, Dominique; Beliaeff, Benoit; Harache, Yves; Loubersac, Lionel; Patrois, Jacques

    2009-01-01

    Shrimp farming in New Caledonia relies on the culture of a domesticated strain of Litopenaeus stylirostris introduced from Mexico at a time when genetic principles were of little or no consideration. Since then, advances in agriculture and for some aquatic species of importance led caledonian shrimp farmers to reconsider the appropriateness of a genetic improvement strategy adapted to local biotechnical and economical constraints. This questioning involves many different and interrelated aspe...

  10. Probiotic P-acidilactici application in shrimp Litopenaeus stylirostris culture subject to vibriosis in New Caledonia

    OpenAIRE

    Castex, Mathieu; Chim, Liet; Pham, Dominique; Lemaire, Pierrette; Wabete, Nelly; Nicolas, Jean-Louis; Schmidely, P; Mariojouls, C.

    2008-01-01

    We studied the effects of a lactic acid bacterium, Pediococcus acidilactici (strain MA 18/5M, CNCM), as a dietary probiotic on growth performance and some nutritional and microbiological aspects of the shrimp Litopenaeus stylirostris. Experimental shrimp culture was carried out over 10 weeks, using floating cages of 14 m(2) each set in earthen ponds, in a New Caledonia farm affected by "Summer syndrome", a septicaemic vibriosis caused by Vibrio nigripulchritudo. The experiment design testing ...

  11. Searching for genetic markers of virulence in Vibrio nigripulchritudo, a Litopenaeus stylirostris shrimp pathogen

    OpenAIRE

    Reynaud, Yann

    2007-01-01

    Since 1997, a new pathology seasonally occurs in new caledonian shrimp farms during the warm season. Diseased Litopenaeus stylirostris shrimp suffer from a systemic vibriosis which was attributed to highly pathogenic Vibrio nigripulchritudo. At the present time, only two farms among 17 are affected by the so called "summer syndrome". In such a context it appears urgent to develop reliable diagnostic tools to detect V. nigripulchritudo strains and to differentiate highly virulent strains from ...

  12. Optimization of the production of shrimp waste protein hydrolysate using microbial proteases adopting response surface methodology

    OpenAIRE

    Dey, Satya S.; Dora, Krushna Chandra

    2011-01-01

    Protein hydrolysates were produced from shrimp waste mainly comprising head and shell of Penaeus monodon by enzymatic hydrolysis for 90 min using four microbial proteases (Alcalase, Neutrase, Protamex, Flavourzyme) where PR(%) and DH (%) of respective enzymes were compared to select best of the lot. Alcalase, which showed the best result, was used to optimize hydrolysis conditions for shrimp waste hydrolysis by response surface methodology using a central composite design. A model equation wa...

  13. Characterisation of Some Immune Genes in the Black Tiger Shrimp, Penaeus monodon

    OpenAIRE

    Sritunyalucksana, Kallaya

    2001-01-01

    The molecular mechanisms of the immune system in shrimp, Penaeus monodon, are completely unknown, despite its economic importance as an aquaculture species, especially in Asia and Latin America. The genes and their gene products involved in the prophenoloxidase activating system, which is considered to be a non-self recognition and defence system in many invertebrates, have been isolated and characterised in shrimp. These include a zymogen of this cascade, prophenoloxidase (proPO); a cell adh...

  14. Brine shrimp lethality and acute oral toxicity studies on Swietenia mahagoni (Linn.) Jacq. seed methanolic extract

    OpenAIRE

    Geethaa Sahgal; Surash Ramanathan; Sreenivasan Sasidharan; Mohd. Nizam Mordi; Sabariah Ismail; Sharif Mahsufi Mansor

    2010-01-01

    Background: The seeds of Swietenia mahagoni have been applied in folk medicine for the treatment of hypertension, diabetes, malaria, amoebiasis, cough, chest pain, and intestinal parasitism. Here we are the first to report on the toxicity of the Swietenia mahagoni crude methanolic (SMCM) seed extract. Methods: SMCM seed extract has been studied for its brine shrimp lethality and acute oral toxicity, in mice. Results: The brine shrimp lethality bioassay shows a moderate cytotoxicity at high co...

  15. Comparing the potential for dispersal via waterbirds of a native and an invasive brine shrimp

    OpenAIRE

    Sánchez, Marta I.; Hortas, F.; Figuerola, Jordi; Green, Andy J.

    2012-01-01

    Migratory waterbirds are likely to have a major role in the spread of many exotic aquatic invertebrates by passive dispersal. However, in the field, this has so far only been confirmed in the case of the American brine shrimp Artemia franciscana, which is spreading quickly around the Mediterranean region. We compared experimentally the capacity of A. franciscana and the native brine shrimp Artemia parthenogenetica to disperse via migratory shorebirds. After Artemia resting eggs (cysts) were f...

  16. A comparison between two brine shrimp assays to detect in vitro cytotoxicity in marine natural products

    OpenAIRE

    Pérez Pilar; Hernández-Inda Zaira L; Carballo José; García-Grávalos María D

    2002-01-01

    Abstract Background The brine shrimp lethality assay is considered a useful tool for preliminary assessment of toxicity. It has also been suggested for screening pharmacological activities in plant extracts. However, we think that it is necessary to evaluate the suitability of the brine shrimp methods before they are used as a general bio-assay to test natural marine products for pharmacological activity. Material and Methods The bioactivity of the isopropanolic (2-PrOH) extracts of 14 specie...

  17. Signalling by the cleaner shrimp Periclimenes longicarpus

    OpenAIRE

    Chapuis, Lucille; Bshary, Redouan

    2012-01-01

    Signals increase the fitness of a sender by altering the behaviour of receivers. For cooperative interactions biological market theory proposes that signalling strength may be linked to supply and demand. In this context, a recent laboratory experiment demonstrated that cleaner shrimps may advertise their service to client reef fish and that the advertisement is linked to hunger levels. We investigated signalling by the cleaner shrimp Periclimenes longicarpus in the field to test more detaile...

  18. Acute toxicity, brine shrimp cytotoxicity, anthelmintic and relaxant potentials of fruits of Rubus fruticosus Agg

    OpenAIRE

    Ali, Niaz; Aleem, Umer; Ali Shah, Syed Wadood; Shah, Ismail; Junaid, Muhammad; Ahmed, Ghayour; Ali, Waqar; Ghias, Mehreen

    2013-01-01

    Background Rubus fruticosus is used in tribal medicine as anthelmintic and an antispasmodic. In the current work, we investigated the anthelmintic and antispasmodic activities of crude methanol extract of fruits of R. fruticosus on scientific grounds. Acute toxicity and brine shrimp cytotoxicity activity of the extract were also performed. Methods Acute toxicity study of crude methanol extract of R. fruticosus was performed on mice. In vitro Brine shrimp cytotoxicity assay was performed on sh...

  19. Leptalpheus pereirai sp. nov., a new alpheid shrimp from Panama and Venezuela (Decapoda: Caridea).

    Science.gov (United States)

    Anker, Arthur; Caripe, Jonathan Vera

    2016-01-01

    A new species of the infaunal alpheid shrimp genus Leptalpheus Williams, 1965 is described based on material from three localities on the Caribbean coast of Panama and Isla Chimana Grande, Venezuela. Leptalpheus pereirai sp. nov. belongs to a group of species characterised by the presence of well-developed adhesive disks on the major chela and appears to dwell in burrows of the large callianassid ghost shrimp, Glypturus acanthochirus Stimpson 1866. PMID:27395620

  20. Application of bacterial lipopolysaccharide to improve survival of the black tiger shrimp after Vibrio harveyi exposure.

    Science.gov (United States)

    Rungrassamee, Wanilada; Maibunkaew, Sawarot; Karoonuthaisiri, Nitsara; Jiravanichpaisal, Pikul

    2013-10-01

    This study investigates an effect of bacterial lipopolysaccharide (LPS) as feed supplement to improve immunity of the black tiger shrimp (Penaeus monodon). LPS was coated to commercial feed pellets and given to the shrimp once or twice a day for 10 days before an exposure with shrimp pathogenic bacterium Vibrio harveyi. The growth rates, percent weight gains, total hemocyte and granulocyte counts and survival rates of shrimp between the LPS-coated pellet fed groups and a control group where shrimp fed with commercial feed pellets were compared. After 10 days of the feeding trials, growth rates were not significantly different in all groups, suggesting no toxicity from LPS supplement. To determine beneficial effect of LPS diets, each group was subsequently exposed to V. harveyi by immersion method and the survival rates were recorded for seven days after the immersion. Regardless of the dosages of LPS, the shrimp groups fed with LPS-coated pellets showed higher survival rates than the control group. There was no significant difference in survival rates between the two LPS dosages groups. In addition to survival under pathogen challenge, we also determine effect of LPS on immune-related genes after 10-day feeding trial. Gene expression analysis in the P. monodon intestines revealed that antilipopolysaccharide factor isoform 3 (ALF3), C-type lectin, and mucine-like peritrophin (mucin-like PM) were expressed significantly higher in a group fed with LPS supplemental diet once or twice a day than in a control group. The transcript levels of C-type lectin and mucin-like PM had increased significantly when LPS was given once a day, while significant induction of ALF3 transcripts was observed when shrimp were fed with LPS twice a day. The up-regulation of the immune gene levels in intestines and higher resistance to V. harveyi of the shrimp fed with LPS provide the evidence for potential application of LPS as an immunostimulant in P. monodon farming. PMID:23751331

  1. Moulds identification and detection of aflatoxin B1 on commercial codiments fermented of shrimp

    Directory of Open Access Journals (Sweden)

    NOOR SOESANTI HANDAJANI

    2006-07-01

    Full Text Available Indonesian tropical climate have an opportunity for fungi growth as Aspergillus flavus Link which can produce aflatoxin within foodstuffs, include condiment of fermented shrimp. Aflatoxin B1 is the dangerous agent having roles as carcinogenic, mutagenic and teratogenic. The aims of this research were known kinds of moulds and detection of aflatoxin B1 on commercial condiments fermented of shrimp. Two brands of commercial condiments fermented of shrimp were taken from traditional markets and supermarkets in Surakarta. Isolation was done by made suspension of sample in aquadets. Suspension on appropriate dilutions was grown on CDA (Czapek’s Dox Agar media with surface spread. The grown colonies were separated and grown on PDA (Potato Dextrose Agar slant media. Furthermore, isolates were cultured on CDA and MEA (Malt Extract Agar media. The grown colonies were microscopes and microscopes examined and identified. Existence of aflatoxin B1 was known by Commercial RIDA Screen ELISA Kit that could detect qualitatively and quantitatively with detection sensitive < 1.7 ppb. Moulds that could be isolated from condiments fermented of shrimp were: Aspergillus flavus Link, Aspergillus niger van Tieghem, Aspergillus wentii Wehmer, Aspergillus PU1 or Aspergillus PU2 and Penicillium citrinum Thom. There was aflatoxin B1 contaminated to 2 brands of commercial condiments fermented of shrimp that were examined. Traditional markets’ commercial condiments fermented of shrimp contained higher aflatoxin B1 than supermarkets’. The brands of commercial condiment of fermented shrimp which had better inner package quality contained lower aflatoxin B1 than the worst inner package quality of commercial condiments of fermented of shrimp.

  2. Multiple host switching events shape the evolution of symbiotic palaemonid shrimps (Crustacea: Decapoda)

    OpenAIRE

    HORKÁ I.; De Grave, S; Fransen, C.H.J.M.; Petrusek, A; Duris, Z.

    2016-01-01

    The majority of the almost 1,000 species of Palaemonidae, the most speciose family of caridean shrimp, largely live in symbioses with marine invertebrates of different phyla. These associations range from weak epibiosis to obligatory endosymbiosis and from restricted commensalism to semi-parasitism, with the specialisation to particular hosts likely playing a role in the diversification of this shrimp group. Our study elucidates the evolutionary history of symbiotic palaemonids based on a phy...

  3. Antimicrobial effect of dietary oregano essential oil against Vibrio bacteria in shrimps

    OpenAIRE

    Gracia-Valenzuela M.H.; Vergara-Jiménez M.J.; Baez-Flores M.E.; Cabrera-Chavez F.

    2014-01-01

    The effect of dietary oregano essential oils on the growth of Vibrio bacteria in shrimps was evaluated. Shrimps were fed: (i) food with oregano oil with a high level of thymol; (ii) food with oregano oil with a high level of carvacrol, and (iii) food without oregano oil (the control). The animals were infected by three species of Vibrio (vulnificus, parahaemolyticus and cholerae). The microbial counts of Vibrio species were significantly lower (p

  4. Determination of total sulfite in shrimp with rapid steam distillation methods

    OpenAIRE

    Vyncke, W.

    1993-01-01

    The efficiency of two types of steam distillation apparatus viz. a rapid distillation unit (Tecator) and a compact distillation apparatus according to Antonacopoulos vas evaluated, using either iodimetric titration or colorimetric determination with Ellman's reagent. There was no interference from the shrimp matrix. Recoveries of sulfite added to shrimp averaged 99,6% (s = 3,8%) for concentrations of at least 3 µg/g with Tecator and 10 /µg/g with Antonacopoulos. Best results were obtained wit...

  5. Bacterial communities associated with white shrimp (Litopenaeus vannamei) larvae at early developmental stages

    OpenAIRE

    ANTONIUS SUWANTO; YULIN LESTARI; MAGGY TENNAWIJAYA SUHARTONO; ARTINI PANGASTUTI

    2010-01-01

    Bacterial communities associated with white shrimp (Litopenaeus vannamei) larvae at early developmental stages. Biodiversitas 11 (2): 65-68.Terminal Restriction Fragment Length Polymorphism (T-RFLP) was used to monitor the dynamics of the bacterial communities associated with early developmental stages of white shrimp (Litopenaeus vannamei) larvae. Samples for analysis were egg, hatching nauplii, 24 hours old nauplii, and 48 hours old nauplii which were collected from one cycle of production ...

  6. Biotechnological process of chitin recovery from shrimp waste using Lactobacillus plantarum NCDN4

    OpenAIRE

    Le, Thanh Ha; Nguyen, Thi Ha

    2015-01-01

    Chitin in shrimp waste is tightly associated with proteins, lipids, pigments and mineral deposits. Therefore, these source materials have to be pretreated to remove these components. For a long time, chemical process has been used widely for extraction of chitin from shrimp waste. The chemical process however led to severe environmental damage and low chitin quality. The biological process has been shown promising to replace the harsh chemical process to reduce the environment impact. In our ...

  7. New aspects of the use of inert diets for high density culturing of brine shrimp

    OpenAIRE

    Dobbeleir, J.; Adam, N.(Princeton University, Princeton, U.S.A); Bossuyt, E.; Bruggeman, E.; Sorgeloos, P

    1980-01-01

    Brine shrimp being non-selective filter-feeders, cheap agricultural waste products should be evaluated as potential food sources for their controlled mass culturing. Preliminary results of screening tests performed with wheat bran, soybean meal , rice bran and whey powder in culturing systems of various size are reported. Wheat bran appears to be unsuitable as monodiet for brine shrimp. The other products tested support good growth although specific precautions have to be taken with some food...

  8. Effect of Probiotics on the Hatchery Seed Production of Black Tiger Shrimp, Penaeus monodon (Fabricius)

    OpenAIRE

    P. Soundarapandian; Babu, R

    2010-01-01

    In recent years, the diseases of shrimps hindered the development of shrimp culture. Hence, the use of probiotic bacteria in aquaculture has tremendous scope and the study of the application of probiotics in aquaculture has a glorious future. In the present study, the probiotics was applied (experimental) for the larval rearing of P. monodon which is compared with control tanks (without probiotics). The temperature and alkalinity of both control and experimental tanks were more of less same. ...

  9. Preliminary Study of BAC Library Construction in Black Tiger Shrimp, Penaeus monodon

    OpenAIRE

    Suwit WUTHISUTHIMETHAVEE; Aoki, Takashi; Hirono, Ikuo; Tassanakajon, Anchalee

    2009-01-01

    Availability of shrimp genome information is necessary for shrimp genetic studies and large-insert DNA clones, bacterial artificial chromosome (BACs) serve as valuable tools for obtaining genomic sequences. The construction of a BAC library was achieved from this preliminary study of P. monodon. High molecular weight (HMW) genomic DNA was isolated from abdominal muscle and the resulting hemocytes were of high quality and sufficient quantity for a BAC library construction. This BAC library was...

  10. COLONIZATION OF MANGROVE FOREST AT ABANDONED SHRIMP-POND OF SEGARA ANAKAN-CILACAP

    OpenAIRE

    Tjut Sugandawaty Djohan

    2015-01-01

    During the 1996 to 1997, large areas of mangrove forest in the Segara Anakan were cleared and converted into intensive shrimp-ponds. After one to two years, these shrimp-ponds failed and were abandoned. These abandoned ponds created large gap areas and canopy gaps, which were colonized by mangrove shrub and liana. The Segara Anakan mangrove also experienced heavy siltation, and there were tree cuttings from the remnant of the mangrove trees. This research aimed to study the colonization of ma...

  11. BRINE SHRIMP LETHALITY BIOASSAY OF GLAUCIUM GRANDIFLORUM VAR. GRANDIFLORUM

    OpenAIRE

    A. SARI, Ç. ÜNSAL, İ. SARIOĞLU, A. SARI, Ç. ÜNSAL, İ. SARIOĞLU

    2013-01-01

    Türkiye'nin 3 farklı bölgesinden toplanan Glaucium grandiflorum Boiss. et Huet var. grandiflorum örneklerinin toprak üstü kısımlarından elde edilen alkaloit ekstreleri ve bu ekstrelerden elde edilen majör alkaloitler allokriptopin, protopİn, (+)-izokoridin, (+)-korİdin üzerinde brİne shrimp lethality testi yapılarak sitotoksisiteleri İncelenmiştir. Glaucium grandiflorum var. grandiflorum türünün 3 örneği de önemli oranda sitotoksik aktİvite göstermiştir. Allokriptopin, protopin, (+)-izokoridi...

  12. Diseases of the eye of farmed shrimp Penaeus monodon.

    Science.gov (United States)

    Smith, P T

    2000-12-21

    Lesions were found in the eyes of cultured shrimp Penaeus monodon that displayed non-specific signs of disease, including lethargy, dark pigmentation, brown gills, empty midgut, anorexia, white tail muscle, necrosis of uropods and fouled cuticle. Eye lesions were associated with sexual development in moribund shrimp in at least 1 disease event. Suppurative inflammation, granuloma and malacia were observed in histological examination of the eye and the causative agents of lesions appear to be Vibrio spp. and a rod-shaped virus (similar to Lymphoid Organ Virus, Gill-Associated Virus [GAV] and Yellow-Head Virus). Suppurative inflammation was characterised by edema, infiltration of haemocytes and local sites of abscesses. Eyes with granuloma usually appeared white in pond-side examinations, and histology showed that fibrous tissue replaced ommatidia, ganglia and internal structures of the eye. Malacia of the eye was characterised by necrosis of nervous tissue, vacuolation and vascular proliferation in the medulla ganglia. Levels of presumptive Vibrionaceace were high in moribund specimens and Gram-negative rods were observed in some specimens as free particles in the interstitial fluid and haemolymph in the eye. Transmission electron microscopy showed that nerve cells in the fasciculated zone (near the basement membrane) contained cytoplasmic vesicles (1 to 3 microm in diameter) with particles (15 to 26 nm in diameter) and rod-shaped nucleocapsids. The rods were similar to those of GAV and were 130 to 260 nm long, 10 to 16 nm in diameter and had helical symmetry with a screw-like thread (2.4 to 3.5 nm pitch). Also, unidentified enveloped virions, averaging 74 nm in diameter, were observed in cytoplasmic vesicles in the fasciculated zone. In conclusion, it is suggested that bacterial and viral infections of the eye could result in impaired neuroendocrine functions, which may cause a range of clinical signs of disease. PMID:11206731

  13. Proteomic Analyses of the Shrimp White Spot Syndrome Virus

    Institute of Scientific and Technical Information of China (English)

    Yan-wei TAN; Zheng-li SHI

    2008-01-01

    White spot syndrome virus (WSSV), a unique member within the virus family Nimaviridae, is the most notorious aquatic virus infecting shrimp and other crustaceans and has caused enormous economic losses in the shrimp farming industry worldwide. Therefore, a comprehensive understanding of WSSV morphogenesis, structural proteins, and replication is essential for developing prevention measures of this serious parasite. The viral genome is approximately 300kb and contains more than 180 open reading frames (ORF). However, most of proteins encoded by these ORF have not been characterized. Due to the importance of WSSV structural proteins in the composition of the virion structure, infection process and interaction with host cells, knowledge of structural proteins is essential to understanding WSSV entry and infection as well as for exploring effective prevention measures. This review article summarizes mainly current investigations on WSSV structural proteins including the relative quantities, localization, function and protein-protein interactions. Traditional proteomic studies of 1D or 2D gel electrophoresis separations and mass spectrometry (MS) followed by database searches have identified a total of 39 structural proteins. Shotgun proteomics and iTRAQ were initiated to identify more structural proteins. To date, it is estimated that WSSV is assembled by at least 59 structural proteins, among them 35 are defined as the envelope fraction (including tegument proteins) and 9 as nucleocapsid proteins. Furthermore, the interaction within several major structural proteins has also been investigated. This identitification and characterization of WSSV protein components should help in the understanding of the viral assembly process and elucidate the roles of several major structural proteins.

  14. Multimeric hemoglobin of the Australian brine shrimp Parartemia.

    Science.gov (United States)

    Coleman, M; Matthews, C M; Trotman, C N

    2001-04-01

    The hemoglobin molecule of the commercially important brine shrimp Artemia sp. has been used extensively as a model for the study of molecular evolution. It consists of nine globin domains joined by short linker sequences, and these domains are believed to have originated through a series of duplications from an original globin gene. In addition, in Artemia, two different polymers of hemoglobin, called C and T, are found which differ by 11.7% at the amino acid level and are believed to have diverged about 60 MYA. This provides a set of data of 18 globin domain sequences that have evolved in the same organism. The pattern of amino acid substitution between these two polymers is unusual, with pairs of equivalent domains displaying differences of up to 2.7-fold in total amino acid substitution. Such differences would reflect a similar range of molecular-clock rates in what appear to be duplicate, structurally equivalent domains. In order to provide a reference outgroup, we sequenced the cDNA for a nine-domain hemoglobin (P) from another genus of brine shrimp, Parartemia zietziana, which differs morphologically and ecologically from Artemia and is endemic to Australia. Parartemia produces only one hundredth the amount of hemoglobin that Artemia produces and does not upregulate production in response to low oxygen partial pressure. Comparison of the globin domains at the amino acid and DNA levels suggests that the Artemia globin T gene has accumulated substitutions differently from the Parartemia P and Artemia C globin genes. We discuss the questions of accelerated evolution after duplication and possible functions for the Parartemia globin. PMID:11264409

  15. Chinese Food in America

    OpenAIRE

    Jou, Diana T.

    2011-01-01

    How did Chinese food get to look like this? With more than 41,000 Chinese restaurants in America - 3 times the number of McDonald’s restaurants - Chinese food is one of the most accepted and misunderstood cuisines in the United States. From large cities to small towns, locals can always count on an order of orange chicken in a takeout box, with a few fortune cookies thrown in the bag. But what Americans view as Chinese food is far from a traditional Chinese meal, wh...

  16. Danish-accented Chinese

    DEFF Research Database (Denmark)

    Wang, Lei; Sloos, Marjoleine 莱娜; Zhang, Chun

    In search for a linguistic basis for the education of Chinese as a foreign language CFL in Denmark, we set up a new line of investigation into CFL. This research focuses on the phonetics and phonology of Mandarin Chinese as compared to Danish. Considering the sound systems of both languages, we...... note some differences and similarities. The most remarkable differences are: -Chinese has rhotic sounds (pinyin ch, zh, sh, r) but Danish does not -Chinese has affricates (c z ch zh tɕ j) but Danish does not What Danish shares with Chinese is the contrast between aspirated and plain consonants: pa...

  17. RECOVERY OF CHITIN AND CHITOSAN FROM SHRIMP WASTE BY CHEMICAL AND MICROBIAL METHODS

    Directory of Open Access Journals (Sweden)

    A. Khanafari, R. Marandi, Sh. Sanatei

    2008-01-01

    Full Text Available Shrimp waste is the most important chitin source for commercial use. In this study chitin and chitosan were extracted from Penaeus semisulcatus waste collected from a shrimp processing landing center situated at Persian Gulf in south of Iran by chemical and microbial methods. Chitin and chitosan were extracted by alkali-acid treatment and the yields were 510 and 410mg/g, respectively. Demineralization is an important step in the chitin purification process from shrimp waste. Chemical extraction method included the use of NaOH solution and acetic acid. In microbial extraction, organic acids (lactic acid produced by probiotic bacteria was used to demineralize microbial deproteinized shrimp shells. The study showed that the effectiveness of using lactic acid bacteria especially added Fe (NO33 as extra nitrogen source for demineralization of shrimp shells than chemical method (1750 against 810mg/g. Chitin and chitosan extracted from shrimp waste by chemical and microbial methods was crystalline powder, non-harmful and odorless, white and off-white, respectively. The moisture content was calculated as 63.8%. The amount of Ca, Fe, Cu and Mn present in the shells was 168, 35.58, 38.28 and 6.72mg/L, obtained by atomic absorption spectroscopy, respectively. The amount of calcium in the shells was 25 times higher than manganese. The results suggested Lactobacillus plantarum (PTTC 1058 is an attractive source of recovery for chitin and chitosan.

  18. Effect of stocking density on extensive production of freshwater shrimp in coal mine reclamation ponds

    International Nuclear Information System (INIS)

    The use of post-mining reclamation ponds for the production of freshwater shrimp was evaluated by examining different stocking densities. Juvenile shrimp (Macrobrachium rosenbergii) averaging 0.5g each were stocked into four existing ponds at Peabody's Ken Surface Mine at 6,175; 12,350; 18,500; and 24,700/ha (2,500, 5,000, 7,500, and 10,000/acre) on June 1, 1995. Shrimp were fed twice a week for 103 days, with harvest conducted September 13, 1995. Survival averaged 40%, overall. Average individual weight size was inversely related to stocking density ranging from 52 g (8.7 shrimp/lb) at 6,175/ha to 20.3 g (22.7 shrimp/lb) at 18,500/acre. Total production was directly related to stocking density ranging from 97 kg/ha (86 lbs/acre) at low density to 211 kg/ha (188 lbs/acre) at 18,500/ha shrimp acre. The major difficulty was at harvest due to difficulty in draining ponds. Construction of designed culture ponds with gravity drains during reclamation could greatly enhance survival, harvestability, and commercial feasibility

  19. Acute Toxicity and Neurotoxicity of Chlorpyrifos in Black Tiger Shrimp, Penaeus monodon

    Directory of Open Access Journals (Sweden)

    Tassanee Eamkamon

    2012-01-01

    Full Text Available Acute toxicity and neurotoxicity of chlorpyrifos were determined in black tiger shrimp, P. monodon. LC50 values after 24 to 96 h of exposure were between 149.55 and 59.16 nmol/L. To determine the neurotoxicity of chlorpyrifos, the inhibition of acetylcholinesterase was monitored in the gill of the shrimps exposed to lethal (0.019, 0.194, and 1.942 µmol/L and sub-lethal (0.019, 0.194, and 1.942 nmol/L concentrations of chlorpyrifos. In lethal dose exposure, the AChE activities observed in shrimp exposed to 0.194, and 1.942 µmol/L of chlorpyrifos were significantly lower (1.7 and 3.3 times than that of control shrimp after 30 min of exposure (p<0.05. In sub-lethal exposure tests, the AChE activity of shrimp was significantly lower (1.9 times than that of control shrimp after exposure to 1.942 nmol/L of chlorpyrifos for 72 h (p<0.05. The sensitive reduction of AChE activity at the sub-lethal concentration, which was 30 times lower than 96 h LC50 value found in this study, indicates the potential use as a biomarker of chlorpyrifos exposure.

  20. Knowledge and innovation relationships in the shrimp industry in Thailand and Mexico.

    Science.gov (United States)

    Lebel, Louis; Garden, Po; Luers, Amy; Manuel-Navarrete, David; Giap, Dao Huy

    2016-04-26

    Experts, government officials, and industry leaders concerned about the sustainability of shrimp aquaculture believe they know what farmers need to know and should be doing. They have framed sustainability as a technical problem that, at the farm level, is to be solved by better shrimp and management of ponds and businesses. Codes of conduct, standards, and regulations are expected to bring deviant practices into line. Shrimp farmers are often cornered in a challenging game of knowledge in which their livelihoods are at stake. In the commodity chain there are multiple relations with both suppliers and buyers, not all of which are trustworthy. The social networks shrimp farmers belong to are crucial for sifting out misinformation and multiplying insights from personal experience in learning by doing. Successful farmers become part of a learning culture through seminars, workshops, and clubs in which knowledge and practices are continually re-evaluated. The combination of vertical and horizontal relationships creates a set of alternative arenas that together are critical to bridging knowledge and action gaps for shrimp farmers. Government and industry initiatives for improving links between knowledge and practice for sustainability have largely succeeded when incentives are aligned: shrimp grow better in healthy environments, and using fewer resources means higher profits. PMID:19892739

  1. The Effect of Three Culture Methods on Intensive Culture System of Pacific White Shrimp (Litopenaeus vannamei)

    Institute of Scientific and Technical Information of China (English)

    MA Zhen; WAN Rong; SONG Xiefa; GAO Lei

    2013-01-01

    Different culture methods may affect the intensive culture system of Pacific white shrimp (Litopenaeus vannamei) regarding water quality and growth and economic performance.This study evaluated the potential effects of three culture methods through cultivation of juvenile shrimps under consistent tank management conditions for 84d.The three methods involved shrimp cultivation in different tanks,i.e.,outdoor tanks with cement bottom (mode-C),greenhouse tanks with cement bottom (mode-G) and outdoor tanks with mud-substrate (mode-M).Results showed that water temperature was significantly higher in mode-G than that in mode-C (P <0.05).In contrast to the other two treatments,mode-M had stable pH after 50d cultivation of shrimps.In the mid-late period,the average concentrations of TAN,NO2-N,DIP and COD were significantly lower in mode-M and mode-G compared with those in mode-C (P <0.05).Despite lack of differences in the final shrimp weight among different treatments (P >0.05),mode-M had significantly higher shrimp yield,survival rate and feed conversion rate (P < 0.05) than other modes.There were significant differences in revenue and net return among different treatments (P<0.05).These demonstrated that the treatments of mode-G and mode-M were conductive to the intensive culture system ofL.vannamei.

  2. The Origin of Large-Bodied Shrimp that Dominate Modern Global Aquaculture.

    Directory of Open Access Journals (Sweden)

    Javier Robalino

    Full Text Available Several shrimp species from the clade Penaeidae are farmed industrially for human consumption, and this farming has turned shrimp into the largest seafood commodity in the world. The species that are in demand for farming are an anomaly within their clade because they grow to much larger sizes than other members of Penaeidae. Here we trace the evolutionary history of the anomalous farmed shrimp using combined data phylogenetic analysis of living and fossil species. We show that exquisitely preserved fossils of †Antrimpos speciosus from the Late Jurassic Solnhofen limestone belong to the same clade as the species that dominate modern farming, dating the origin of this clade to at least 145 mya. This finding contradicts a much younger Late Cretaceous age (ca. 95 mya previously estimated for this clade using molecular clocks. The species in the farmed shrimp clade defy a widespread tendency, by reaching relatively large body sizes despite their warm water lifestyles. Small body sizes have been shown to be physiologically favored in warm aquatic environments because satisfying oxygen demands is difficult for large organisms breathing in warm water. Our analysis shows that large-bodied, farmed shrimp have more gills than their smaller-bodied shallow-water relatives, suggesting that extra gills may have been key to the clade's ability to meet oxygen demands at a large size. Our combined data phylogenetic tree also suggests that, during penaeid evolution, the adoption of mangrove forests as habitats for young shrimp occurred multiple times independently.

  3. The Origin of Large-Bodied Shrimp that Dominate Modern Global Aquaculture

    Science.gov (United States)

    Wilkins, Blake; Bracken-Grissom, Heather D.; Chan, Tin-Yam; O’Leary, Maureen A.

    2016-01-01

    Several shrimp species from the clade Penaeidae are farmed industrially for human consumption, and this farming has turned shrimp into the largest seafood commodity in the world. The species that are in demand for farming are an anomaly within their clade because they grow to much larger sizes than other members of Penaeidae. Here we trace the evolutionary history of the anomalous farmed shrimp using combined data phylogenetic analysis of living and fossil species. We show that exquisitely preserved fossils of †Antrimpos speciosus from the Late Jurassic Solnhofen limestone belong to the same clade as the species that dominate modern farming, dating the origin of this clade to at least 145 mya. This finding contradicts a much younger Late Cretaceous age (ca. 95 mya) previously estimated for this clade using molecular clocks. The species in the farmed shrimp clade defy a widespread tendency, by reaching relatively large body sizes despite their warm water lifestyles. Small body sizes have been shown to be physiologically favored in warm aquatic environments because satisfying oxygen demands is difficult for large organisms breathing in warm water. Our analysis shows that large-bodied, farmed shrimp have more gills than their smaller-bodied shallow-water relatives, suggesting that extra gills may have been key to the clade’s ability to meet oxygen demands at a large size. Our combined data phylogenetic tree also suggests that, during penaeid evolution, the adoption of mangrove forests as habitats for young shrimp occurred multiple times independently. PMID:27415002

  4. PREDICTABLE AND PRICE VOLATILITY RISK IN THE BRAZILIAN MARKET INTEGRATION OF SHRIMP

    Directory of Open Access Journals (Sweden)

    Israel José dos Santos Felipe

    2015-12-01

    Full Text Available The present paper has the purpose of investigate the dynamics of the volatility structure in the shrimp prices in the Brazilian fish market. Therefore, a description of the initial aspects of the shrimp price series was made. From this information, statistics tests were made and selected univariate models to be price predictors. It´s presented as an exploratory research of applied nature with quantitative approach. The database was collected through direct contact with the Society of General Warehouses of São Paulo (CEAGESP.The results showed that the great variability in the active price is directly related with the gain and loss of the market agents. The price series presents a strong seasonal and biannual effect. The average structure of price of shrimp in the last 12 years was R$ 11.58 and external factors besides the production and marketing (U.S. antidumping, floods and pathologies strongly affected the prices. Among the tested models for predicting prices of shrimp, four were selected, which through the prediction methodologies of "One Step Ahead" with 12 periods horizon , proved to be statistically more robust. We concluded that the dynamic pricing of commodity shrimp is strongly influenced by external productive factors and that these phenomena cause seasonal effects in the prices. Through statistical modeling is possible to minimize the risk and uncertainty embedded in the fish market, thus, the sales and marketing strategies for the Brazilian shrimp can be consolidated and widespread.

  5. Knowledge and innovation relationships in the shrimp industry in Thailand and Mexico

    Science.gov (United States)

    Lebel, Louis; Garden, Po; Luers, Amy; Manuel-Navarrete, David; Giap, Dao Huy

    2016-01-01

    Experts, government officials, and industry leaders concerned about the sustainability of shrimp aquaculture believe they know what farmers need to know and should be doing. They have framed sustainability as a technical problem that, at the farm level, is to be solved by better shrimp and management of ponds and businesses. Codes of conduct, standards, and regulations are expected to bring deviant practices into line. Shrimp farmers are often cornered in a challenging game of knowledge in which their livelihoods are at stake. In the commodity chain there are multiple relations with both suppliers and buyers, not all of which are trustworthy. The social networks shrimp farmers belong to are crucial for sifting out misinformation and multiplying insights from personal experience in learning by doing. Successful farmers become part of a learning culture through seminars, workshops, and clubs in which knowledge and practices are continually re-evaluated. The combination of vertical and horizontal relationships creates a set of alternative arenas that together are critical to bridging knowledge and action gaps for shrimp farmers. Government and industry initiatives for improving links between knowledge and practice for sustainability have largely succeeded when incentives are aligned: shrimp grow better in healthy environments, and using fewer resources means higher profits. PMID:19892739

  6. Advances, challenges, and directions in shrimp disease control: the guidelines from an ecological perspective.

    Science.gov (United States)

    Xiong, Jinbo; Dai, Wenfang; Li, Chenghua

    2016-08-01

    High-density aquaculture has led to increasing occurrences of diseases in shrimp. Thus, it is imperative to establish effective and quantitative strategies for preventing and predicting these diseases. Water quality indices and investigations of specific pathogen abundance provide only a qualitative evaluation of the risk of shrimp disease and can be inaccurate. To address these shortcomings, we introduced intestinal indicative assemblages as independent variables with which to quantitatively predict incidences of shrimp disease. Given the ignorance regarding the niches differences in the shrimp intestine throughout its developmental stages, the use of probiotics in aquaculture has had limited success. Therefore, we propose the exploration of effective probiotic bacteria from shrimp intestinal flora and the establishment of therapeutic strategies dependent on shrimp age. Following ecological selection principles, we hypothesize that the larval stage provides the best opportunity to establish a desired gut microbiota through preemptive colonization of the treated rearing water with known probiotics. To employ this strategy, however, substantial barriers must be overcome. PMID:27333908

  7. ADOPTION OF GOOD MANAGEMENT PRACTICE (GMP) IN SMALL AND MEDIUM SCALE VANNAMEI SHRIMP FARMS ON THE NORTHERN SHORE EAST JAVA

    OpenAIRE

    Paula Florina; Sri Hartoyo; Sukardi Sukardi

    2012-01-01

    The objectives of this research were to describe the value chain of Vannamei shrimp industry on the northern shore of East Java, to identify the current management practice that is being implemented by small to medium scale shrimp farmers, to analyze the level of adoption of Good Management Practice by small to medium scale shrimp farmers and to identify the challenges that prevent small to medium scale farmers from adopting Good Management Practice. This research utilized descriptive qualita...

  8. Source identification and entry pathways of banned antibiotics nitrofuran and chloramphenicol in shrimp value chain of Bangladesh

    OpenAIRE

    M. Jakiul Islam; Afroza Akter Liza; A.H.M. Mohsinul Reza; M. Shaheed Reza; Mohammed Nurul Absar Khan; Md. Kamal

    2014-01-01

    Background: Contamination with residues of banned carcinogenic antibiotic drugs like nitrofuran metabolites and chloramphenicol (CAP) in frozen shrimp products has become a major concern of food safety for exporting countries. In the present study an approach was taken to identify the sources of such harmful antibiotics in the shrimp value chain of Bangladesh, one of the major shrimp countries. Materials and Methods: Inputs of farms and hatchery systems including feed, feed additives, fee...

  9. Feasibility of polyculture of blue shrimp Litopenaeus stylirostris and goldlined rabbitfish Siganus lineatus in a mesocosm system

    OpenAIRE

    Luong, Trung Cong; Hochard, Sebastien; Royer, Florence; Lemonnier, Hugues; Letourneur, Yves

    2014-01-01

    The study was conducted to (1) estimate the effects of polyculture of blue shrimp Litopenaeus stylirostris with goldlined rabbitfish Siganus lineatus on production, environmental quality and water and sediment metabolism and (2) to determine if blue shrimp and goldlined rabbitfish polyculture is possible. The experiment was carried out for 12 weeks in a mesocosm system that consisted of 12 circular outdoor mesocosm fiberglass tanks (1.7 m2, 1275 L water volume). Shrimp (2.9 g) were randomly s...

  10. Biological, physiological, immunological and nutritional assessment of farm-reared Litopenaeus stylirostris shrimp affected or unaffected by vibriosis

    OpenAIRE

    Mugnier, Chantal; Justou, Carole; Lemonnier, Hugues; Patrois, Jacques; Ansquer, Dominique; Goarant, Cyrille; Le Coz, Jean-rene

    2013-01-01

    Shrimp aquaculture in New Caledonia is subject to seasonal mortalities during grow-out due to highly virulent Vibrio nigripulchritudo (Vn). To understand the mechanisms affecting shrimp resistance and leading to significant mortality, a shrimp ecophysiology and immunology survey was conducted on two farms, the first considered as a "control" farm (HC), the second affected by the disease (DF). Mortality observed during the survey at DF started 50 days after stocking and was typical of this dis...

  11. Oxidative stress response of black tiger shrimp (Penaeus monodon) to enrofloxacin and to culture system

    OpenAIRE

    Thi Tu, H.; Silvestre, F.; A. Bernard; Douny, C.; Thanh Phuong, N.; Tai Tao, C.; Maghuin-Rogister, G; KESTEMONT P.

    2008-01-01

    In Vietnam, enrofloxacin is one of the most commonly used antibiotics in shrimp farms. Although the European Union set a maximum residue limit (MRL) of 100 µg/kg for enrofloxacin and its metabolite ciprofloxacin in edible tissues of aquatic animals for human consumption, only few data are available on its potential deleterious effects on shrimp. This study aimed to investigate the impacts of enrofloxacin on oxidative stress in hepatopancreas and gills of black tiger shrimp (Penaeus monodon). ...

  12. Bioencapsulation of the antibacterial drug sarafloxacin in nauplii of the brine shrimp Artemia franciscana

    OpenAIRE

    Dixon, B.A.; Van Poucke, S; Chair, M; Dehasque, M.; Nelis, H J; Sorgeloos, P.; De Leenheer, A P

    1995-01-01

    Nauplii of the brine shrimp Artemia franciscana were enriched with the antibacterial drug sarafloxacin hydrochloride to determine if levels effective against four pathogenetic strains of Vibrio spp. would accumulate in brine shrimp. Three vibrio strains were Vibrio anguillarum; the fourth was V. vulnificus. Concentrations of sarafloxacin ranging from 1 to 40% (weight: volume) were incorporated into the oil phase of Super Selco brine shrimp diets. Enriched nauplii and controls were sampled at ...

  13. Shrimp Farming Practices in the Puttallam District of Sri Lanka: Implications for Disease Control, Industry Sustainability, and Rural Development

    OpenAIRE

    M. Nalaka Munasinghe; Craig Stephen; Preeni Abeynayake; Abeygunawardena, Indra S.

    2010-01-01

    Shrimp farming has great potential to diversify and secure income in rural Sri Lanka, but production has significantly declined in recent years due to civil conflicts, some unsustainable practices and devastating outbreaks of disease. We examined management practices affecting disease prevention and control in the Puttalam district to identify extension services outputs that could support sustainable development of Sri Lankan shrimp farming. A survey on 621 shrimp farms (603 operational and 1...

  14. Impact of the commercial fishery on the population of bait shrimp (Penaeus spp.) in Biscayne Bay, 1986

    OpenAIRE

    Campos, W. L.; Berkeley, S.A.

    2003-01-01

    Monthly population size of bait shrimp in the Bay was estimated from December 1984 to July 1985. Growth rates for male and female P. duorarum showed that pink shrimp exhibit a mean residence time in the nursery area (Biscayne Bay) of approximately 21 weeks. Monthly mortality rates were determined for each sex of pink shrimp. It was estimated that 23% and 26% of the male and female monthly population size, respectively, was absorbed by both the fishery and ecosystem monthly. Monthly prop...

  15. A Demographic Profile of Participants in Two Gulf of Mexico Inshore Shrimp Fisheries and Their Response to the Texas Closure

    OpenAIRE

    Nance, James M.; Garfield, Nina; Paredes, J. Anthony

    1991-01-01

    A social study of the shrimp fisheries of Galveston Bay, Tex., and Calcasieu Lake, La., was made during the summer of 1987 to examine the impacts of the seasonal closure of the Federal waters off Texas and to understand the infrastructure and demographic processes of these two diverse fisheries. Survey instruments were administered to 159 shrimp boat captains: 89 from Galveston Bay and 70 from Calcasieu Lake. Shrimp-house owners were interviewed in each region as well. The results suggest ...

  16. Observations on the biology of the endangered stygobiotic shrimp Palaemonias alabamae, with notes on P. ganteri (Decapoda: Atyidae)

    OpenAIRE

    Martha Cooper; John Cooper

    2011-01-01

    Palaemonias alabamae is endemic to subterranean waters in northern Alabama. Its type locality is Shelta Cave, Madison County, and ostensibly conspecifi c shrimps have been found in Bobcat and two other caves. Pollution and other factors may have extirpated the shrimp from the type locality. In Shelta Cave the species is smaller than the shrimp in Bobcat Cave and P. ganteri in Mammoth Cave, Kentucky. Adult female P. alabamae (s.s.) and P. ganteri are larger than males. Female P. alabamae with ...

  17. Effect of financial and environmental variables on the production efficiency of white leg shrimp farms in Khan Hoa province, Vietnam

    OpenAIRE

    Akter, Shamima

    2010-01-01

    Since 1997, development of white leg shrimp farming in Vietnam has attracted considerable attention due to its high export potential. Therefore, it is now necessary to assess the annual efficiency performance of white leg shrimp farms in order to improve Vietnam’s aquaculture policy. This paper based on a character sample of 61 white leg shrimp farmers in Khanh Hoa province, representing about 13.2% of such farmers in Khanh Hoa, Vietnam. Using an output-oriented VRS Data Envelopment Analysis...

  18. Measurement of pollution levels of organochlorine and organophosphorus pesticides in water, soil, sediment, and shrimp to identify possible impacts on shrimp production at Jiquilisco Bay.

    Science.gov (United States)

    Nomen, Rosa; Sempere, Julià; Chávez, Francisco; de López, Nelly Amaya; Rovira, Ma Dolores

    2012-09-01

    This study aims to identify levels of several organochlorine and organophosphorus compounds in shrimp-raising areas of coastal El Salvador, to assess potential impacts on shrimp growth and survival that hamper the sustainability of aquaculture in the region. The paper reports the current levels of γ-HCH, 4,4'-DDT, 4,4'-DDE, 4,4'-DDD, endrin, dieldrin, heptachlor, parathion, methyl parathion, and etoprophos in soils (depth 20 cm), sediments (depth 5 cm), shrimp (Penaeus sp.), and water of three rearing ponds and also in the sediment (depth 5 cm) and water surrounding those ponds in Jiquilisco Bay. Sampling was carried out during the dry (January-March) and rainy (June-August) seasons of 2008. The presence of pesticides in the samples of water, shrimp, and sediment at shrimp ponds was not detected in either season; however, in soil samples (depth 20 cm) taken from these ponds, heptachlor, endrin, dieldrin, 4,4'-DDD, and 4,4'-DDT were identified at concentrations below the method limit of quantification (LOQ), and 4,4'-DDE was found in a concentration falling in the range from 3.85 to 19.61 ng/g. In samples of water taken at the bay water intakes to the rearing ponds, we observed dieldrin concentrations in the range between 0.085 ng/mL and 0.182 ng/mL during the dry season. In the samples of sediments taken in the surrounding areas of shrimp ponds, we found-for both seasons-that in 60 % of the samples, 4,4'-DDE was present in concentrations ranging from 3.75 ng/g to 30.97 ng/g. Additionally, in the rainy season, we observed heptachlor in sediment at concentrations below the method quantification limit. It was concluded that organochlorine compounds from pesticides are still present in Jiquilisco Bay, trapped in deep sediment, even though they have been banned since the 1980s. These were not detected in shrimp tissue, surface water, and shallow sediment in rearing ponds, and hence, we do not believe their presence has any major impact on shrimp production in sampled

  19. Drying Characteristics and Physical and Nutritional Properties of Shrimp Meat as Affected by Different Traditional Drying Techniques

    Directory of Open Access Journals (Sweden)

    P. T. Akonor

    2016-01-01

    Full Text Available The influence of different drying methods on physical and nutritional properties of shrimp meat was investigated in this study. Peeled shrimps were dried separately using an air-oven dryer and a tunnel solar dryer. The drying profile of shrimp meat was determined in the two drying systems by monitoring moisture loss over the drying period. Changes in color, proximate composition, and rehydration capacity were assessed. The rate of moisture removal during solar drying was faster than the air-oven drying. The development of red color during drying was comparable among the two methods, but solar-dried shrimps appeared darker (L⁎=47.4 than the air-oven-dried (L⁎=49.0. Chemical analysis indicated that protein and fat made up nearly 20% and 2% (wb of the shrimp meat, respectively. Protein and ash content of shrimp meat dried under the two dryer types were comparable but fat was significantly (p<0.05 higher in oven-dried meat (2.1%, compared to solar-dried meat (1.5%. Although rehydration behavior of shrimp from the two drying systems followed a similar pattern, solar-dried shrimp absorbed moisture more rapidly. The results have demonstrated that different approaches to drying may affect the physical and nutritional quality of shrimp meat differently.

  20. Estimating the Economic Impact of the Wild Shrimp, Penaeus sp., Fishery: A Study of Terrebonne Parish, Louisiana

    OpenAIRE

    LaFleur, Elizabeth; Yeates, Diane; Aysen, Angelina

    2005-01-01

    Two approaches are used to estimate the economic impact of domestic wild shrimp, Penaeus sp., fishing in Terrebonne Parish, Louisiana. A 2002 survey of commercial shrimp fishermen in the Parish yields information on sales and operating costs, and results are used to estimate a 1-yr sales effect in the Parish of $36.7 to $128.1 million due to shrimp fishing. In addition, 2001 shrimp ticket sales data ($49.9 million) are input into a REMI (Regional Economic Models, Inc.) model built for t...

  1. Strategy of Quality Improvement of Pond Shrimp Post Harvest Management (Penaeus monodon Fabricius in Mahakam Delta (Case Study

    Directory of Open Access Journals (Sweden)

    Andi Noor Asikin

    2012-10-01

    Full Text Available Mahakam Delta area, which is situated in Kutai Kartanegara Regency, has become a pond center that gives a significant contribution to shrimp export of East Kalimantan Province. Pond-produced shrimps, however, do not always accord to the intention of cold storage companies at expected price. The companies even sometimes reject the request due to poor quality of the product. The decreasing shrimp quality may be due to maintenance process by the pond farmers as well as the improper collectors. In the other hand, importing countries have decided more and more restricted requirements for the imported fishing products. This study was held in Muara Jawa, Anggana, and Muara Badak Districts using techniques of data collection of in-depth interview with twelve respondents. In order to improve shrimp quality, farmers or the producers have to formulate a strategy towards the improvement of the post harvest shrimps from the pond using an Analytical Hierarchy Process (AHP. Results of the AHP consist of as follows: (1 application of pond shrimp post harvest technology and improvement of facilities and accommodation that support the quality improvement of the pond shrimps, (2 human resource quality improvement of pond farmers, collectors, and field instructors, (3 development of pond culture by applying best practices principle and local policies in order to improve the quality of the pond shrimps, and (4 improvement of interagency coordination, monitoring, and evaluation to enhance the pond shrimp quality

  2. ADOPTION OF GOOD MANAGEMENT PRACTICE (GMP IN SMALL AND MEDIUM SCALE VANNAMEI SHRIMP FARMS ON THE NORTHERN SHORE EAST JAVA

    Directory of Open Access Journals (Sweden)

    Paula Florina

    2012-09-01

    Full Text Available The objectives of this research were to describe the value chain of Vannamei shrimp industry on the northern shore of East Java, to identify the current management practice that is being implemented by small to medium scale shrimp farmers, to analyze the level of adoption of Good Management Practice by small to medium scale shrimp farmers and to identify the challenges that prevent small to medium scale farmers from adopting Good Management Practice. This research utilized descriptive qualitative method using multiple-case study. Data collection was conducted through interviews with experts and various actors in shrimp industry and interviews and observations in shrimp farms. From this research it can be concluded that the farming activity gives the largest contribution in final product value, that the current management practice in observed intensive Vannamei shrimp farms is in line with recommendations of various institutions with the exception of waste management, that the observed intensive Vannamei shrimp farms have high adoption level of Good Management Practice (GMP for various stages of shrimp farming activity with the exception of waste management, and that there is a tendency that farmers follow the good practices that have direct impact to the success of the production, and that the main challenges of GMP adoption are the lack of a comprehensive GMP manual, the lack of structured technology dissemination, and the lack of incentive for the farmers to adopt waste management practices.Keywords: Litopenaeus Vannamei, Shrimp Farm, Good Management Practice, Value Chain, Multiple-Case Study

  3. Relating large-scale climate variability to local species abundance: ENSO forcing and shrimp in Breton Sound, Louisiana, USA

    Science.gov (United States)

    Piazza, Bryan P.; LaPeyre, Megan K.; Keim, B.D.

    2010-01-01

    Climate creates environmental constraints (filters) that affect the abundance and distribution of species. In estuaries, these constraints often result from variability in water flow properties and environmental conditions (i.e. water flow, salinity, water temperature) and can have significant effects on the abundance and distribution of commercially important nekton species. We investigated links between large-scale climate variability and juvenile brown shrimp Farfantepenaeus aztecus abundance in Breton Sound estuary, Louisiana (USA). Our goals were to (1) determine if a teleconnection exists between local juvenile brown shrimp abundance and the El Niño Southern Oscillation (ENSO) and (2) relate that linkage to environmental constraints that may affect juvenile brown shrimp recruitment to, and survival in, the estuary. Our results identified a teleconnection between winter ENSO conditions and juvenile brown shrimp abundance in Breton Sound estuary the following spring. The physical connection results from the impact of ENSO on winter weather conditions in Breton Sound (air pressure, temperature, and precipitation). Juvenile brown shrimp abundance effects lagged ENSO by 3 mo: lower than average abundances of juvenile brown shrimp were caught in springs following winter El Niño events, and higher than average abundances of brown shrimp were caught in springs following La Niña winters. Salinity was the dominant ENSO-forced environmental filter for juvenile brown shrimp. Spring salinity was cumulatively forced by winter river discharge, winter wind forcing, and spring precipitation. Thus, predicting brown shrimp abundance requires incorporating climate variability into models.

  4. The known two types of transglutaminases regulate immune and stress responses in white shrimp, Litopenaeus vannamei.

    Science.gov (United States)

    Chang, Chin-Chyuan; Chang, Hao-Che; Liu, Kuan-Fu; Cheng, Winton

    2016-06-01

    Transglutaminases (TGs) play critical roles in blood coagulation, immune responses, and other biochemical functions, which undergo post-translational remodeling such as acetylation, phosphorylation and fatty acylation. Two types of TG have been identified in white shrimp, Litopenaeus vannamei, and further investigation on their potential function was conducted by gene silencing in the present study. Total haemocyte count (THC), differential haemocyte count (DHC), phenoloxidase activity, respiratory bursts (release of superoxide anion), superoxide dismutase activity, transglutaminase (TG) activity, haemolymph clotting time, and phagocytic activity and clearance efficiency to the pathogen Vibrio alginolyticus were measured when shrimps were individually injected with diethyl pyrocarbonate-water (DEPC-H2O) or TG dsRNAs. In addition, haemolymph glucose and lactate, and haemocytes crustin, lysozyme, crustacean hyperglycemic hormone (CHH), transglutaminaseI (TGI), transglutaminaseII (TGII) and clotting protein (CP) mRNA expression were determined in the dsRNA injected shrimp under hypothermal stress. Results showed that TG activity, phagocytic activity and clearance efficiency were significantly decreased, but THC, hyaline cells (HCs) and haemolymph clotting time were significantly increased in the shrimp which received LvTGI dsRNA and LvTGI + LvTGII dsRNA after 3 days. However, respiratory burst per haemocyte was significantly decreased in only LvTGI + LvTGII silenced shrimp. In hypothermal stress studies, elevation of haemolymph glucose and lactate was observed in all treated groups, and were advanced in LvTGI and LvTGI + LvTGII silenced shrimp following exposure to 22 °C. LvCHH mRNA expression was significantly up-regulated, but crustin and lysozyme mRNA expressions were significantly down-regulated in LvTGI and LvTGI + LvTGII silenced shrimp; moreover, LvTGII was significantly increased, but LvTGI was significantly decreased in LvTGI silenced shrimp

  5. Shelf-life extension of dried shrimps by irradiation and packaging techniques

    International Nuclear Information System (INIS)

    Dried shrimps were air-packaged or N2-packaged in the flexible pouch of oriented polypropylene (OPP), polyvinylidene chloride coated polypropylene (KOP) and ethylene vinyl alcohol copolymer (EVOH). The packages were irradiated with gamma ray from cobalt 60 at 3 and 8 kGy. Non-irradiated dried shrimps were used as a control. The samples were stored at 30 deg C and 70+-5 percent RH for 4 months. Non-enzymatic browning induced the discoloration of dried shrimps, and oxidation of astaxanthin proceeded after irradiation and during storage. The hunter color difference, delta E, of all the samples was increased, especially in the non-irradiated air-packages samples. The decrease in astaxanthin content was found in both air - and N2-packaged samples during storage. The number of microorganisms was reduced by irradiation dose of 3 and 8 kGy from 6.5 to 4.2 and 2.7 log CFU/g, respectively. The decreased concentration of O2 in the package also reduced the number of microorganisms. The air packaged dried shrimps in the OPP pouch deteriorated during storage. Shrinkage of the pouches and growth of halophilic bacteria on the surface of dried shrimps were found at a sub(w) of 0.75. The discoloration of dried shrimps in the OPP pouch was more significant than that in other pouches. Irradiation at 3 and 8 kGy in combination with packaging in KOP and EVOH pouches containing N2 atmosphere was one of the promising methods for prolonging shelf-life of dried shrimps at room temperature

  6. Resistance to the crayfish plague pathogen, Aphanomyces astaci, in two freshwater shrimps.

    Science.gov (United States)

    Svoboda, J; Mrugała, A; Kozubíková-Balcarová, E; Kouba, A; Diéguez-Uribeondo, J; Petrusek, A

    2014-09-01

    Aphanomyces astaci, the causal agent of the crayfish plague, has recently been confirmed to infect also freshwater-inhabiting crabs. We experimentally tested the resistance of freshwater shrimps, another important decapod group inhabiting freshwaters, to this pathogen. We exposed individuals of two Asian shrimp species, Macrobrachium dayanum and Neocaridina davidi, to zoospores of the pathogen strain isolated from Procambarus clarkii, a known A. astaci carrier likely to get into contact with shrimps. The shrimps were kept in separate vessels up to seven weeks; exuviae and randomly chosen individuals were sampled throughout the experiment. Shrimp bodies and exuviae were tested for A. astaci presence by a species-specific quantitative PCR. The results were compared with amounts of A. astaci DNA in an inert substrate to distinguish potential pathogen growth in live specimens from persisting spores or environmental DNA attached to their surface. In contrast to susceptible crayfish Astacus astacus, we did not observe mortality of shrimps. The amount of detected pathogen DNA was decreasing steadily in the inert substrate, but it was still detectable several weeks after zoospore addition, which should be considered in studies relying on molecular detection of A. astaci. Probably due to moulting, the amount of A. astaci DNA was decreasing in N. davidi even faster than in the inert substrate. In contrast, high pathogen DNA levels were detected in some non-moulting individuals of M. dayanum, suggesting that A. astaci growth may be possible in tissues of this species. Further experiments are needed to test for the potential of long-term A. astaci persistence in freshwater shrimp populations. PMID:25064254

  7. The potential use of constructed wetlands in a recirculating aquaculture system for shrimp culture

    International Nuclear Information System (INIS)

    Constructed wetlands improved water qualities and consequently increased the shrimp growth and survival in a recirculating system. - A pilot-scale constructed wetland unit, consisting of free water surface (FWS) and subsurface flow (SF) constructed wetlands arranged in series, was integrated into an outdoor recirculating aquaculture system (RAS) for culturing Pacific white shrimp (Litopenaeus vannamei). This study evaluated the performance of the wetland unit in treating the recirculating wastewater and examined the effect of improvement in water quality of the culture tank on the growth and survival of shrimp postlarvae. During an 80-day culture period, the wetland unit operated at a mean hydraulic loading rate of 0.3 m/day and effectively reduced the influent concentrations of 5-day biochemical oxygen demand (BOD5, 24%), suspended solids (SS, 71%), chlorophyll a (chl-a, 88%), total ammonium (TAN, 57%), nitrite nitrogen (NO2-N, 90%) and nitrate nitrogen (NO3-N, 68%). Phosphate (PO4-P) reduction was the least efficient (5.4%). The concentrations of SS, Chl-a, turbidity and NO3-N in the culture tank water in RAS were significantly (P≤0.05) lower than those in a control aquaculture system (CAS) that simulated static pond culture without wetland treatment. However, no significant difference (P≤0.05) in BOD5, TAN and NO2-N was found between the two systems. At the end of the study, the harvest results showed that shrimp weight and survival rate in the RAS (3.8±1.8 g/shrimp and 90%) significantly (P≤0.01) exceeded those in the CAS (2.3±1.5 g/shrimp and 71%). This study concludes that constructed wetlands can improve the water quality and provide a good culture environment, consequently increasing the shrimp growth and survival without water exchange, in a recirculating system

  8. A study on biosorption of copper ions by fungal chitosan: an alternative to shrimp chitosan

    Directory of Open Access Journals (Sweden)

    Sanaz Behnam

    2015-02-01

    Full Text Available   Introduction : One of the main applications of chitosan is for heavy metals removal from waste waters. Industrially, chitosan is produced through deacetylation of chitin present in shellfish waste. Another source of chitosan is the cell wall of zygomycetes fungi with several advantages over shellfish wastes .   Materials and method s: Fungal chitosan purified from biomass of Mucor indicus and shrimp chitosan were applied and compared for removal of copper ions from aqueous solution. The effects of pH (3 to 5.5, copper ion concentration (5 to 52 mg l-1, the amount of chitosan (200 to 3000 mg l-1, adsorption time, temperature, and presence of other metal ions on the biosorption of Cu2+ were investigated .   Results : Maximum adsorption capacities for fungal and shrimp chitosans were 58.5 and 60.7 mg g-1, respectively. T he rate of copper adsorption by the fungal chitosan was significantly higher than that by the shrimp chitosan. Among p seudo-first order, pseudo-second order, intra-particle diffusion, and Elovich models, Ho’s pseudo-second order model was the best model for fitting the kinetic data. The adsorption capacity increased for both types of chitosans by increasing the solution pH. However, temperature and presence of other ions did not show significant effects on the biosorption capacity of copper. The isotherm data were very well described by Langmuir, Freundlich, and Redlich-Peterson models .   Discussion and conclusion : Both fungal and shrimp chitosans can effectively be used for removal of copper ions from aqueous solutions. Adsorption process for fungal chitosan is fast, while the process is slower for the shrimp chitosan. Therefore, from the kinetics point of view, the fungal chitosan is preferable compared with the shrimp chitosan . Key words: Biosorption, Copper, Fungal chitosan, Shrimp chitosan, Water treatment .

  9. Application of Irradiated Pro biotic Microorganism in Black Tiger Shrimp (Penaeus monodon Fabricius) Culture

    International Nuclear Information System (INIS)

    Marine shrimp culture in Thailand has been developed continuously for the past two decades. This development will ensure the highest level of shrimp quality that will be suitable for the consumption of the people in the country and also aboard. The trend of culture system emphasizes on disease prevention more than treatment which will consequently limit the application of drug and chemicals. Application of pro biotic has been one means of this prevention that are commonly practiced by shrimp farmers. This research was conducted to compare the efficacy of normal Bacillus subtilis isolate from shrimp intestine and an irradiated B. subtilis as a pro biotic in shrimp feed. It was found that overall results were quite the same. These included the broth Co-culture assay. Effects on immune functions were conducted with Penaeus monodon with initial average weight of 17 gms by feeding with 3 gms/kg feed of spore of these two pro biotic for two mouths. The results indicated that both pro biotic caused significant improvement on percent phagocytosis only at the forth week of feeding trial and the overall enhancement of bactericidal activity. However, total haemocyte count and phenoloxidase activity were not altered. Total bacterial count in shrimp intestine was also conducted during the two month trial. the results indicated significant reduction of Vibrio spp. of both pro biotic groups when compared with the control. Number of Bacillus spp. in intestine were continuously high even after pro biotic treatment had been stopped Growth rate of experiment and control shrimp was not significantly different.

  10. Uptake and retention of 124Sb in the common mussel, shrimp and shore crab

    International Nuclear Information System (INIS)

    The uptake of radioactive antimony from water and food by mussels, shrimps and shore crabs and the subsequent loss in non-radioactive sea water were studied with 124Sb. The concentration factors of about 0.6 for mussels and 2.5 for shrimps, reached by direct uptake from sea water, remained considerably below stable-antimony concentration factors reported for these organisms. The loss of 124Sb after uptake of the radionuclide by mussels during 19 and 32 days respectively, could be described by a loss from two compartments with different rates. The distribution of the radionuclide in the animals did not change during the retention period. Antimony-124 taken up from sea water by shrimps was largely accounted for by adsorption to the exoskeleton, as was shown by the effect of moulting on the time course of subsequent loss in non-radioactive sea water. Antimony-124 applied to freeze-dried mussel flesh that was fed to shrimps was lost according to a single exponential function. The mean biological half-life of 124Sb elimination was about 10 days. Two components were shown to be present in the retention of 124Sb in two groups of shore crabs, one of which was fed freeze-dried shrimps spiked with 124Sb, while the second group received shrimps labelled by uptake of 124Sb with food. The only significant difference between the two groups was a longer mean biological half-life of the short-lived component in the second group. In shrimps and crabs only a small fraction of 124Sb taken up with food ends up in the exoskeleton. About 45% of retained activity in crabs was found in the digestive gland. (author)

  11. The potential use of constructed wetlands in a recirculating aquaculture system for shrimp culture

    Energy Technology Data Exchange (ETDEWEB)

    Lin, Y.-F.; Jing, S.-R.; Lee, D.-Y

    2003-05-01

    Constructed wetlands improved water qualities and consequently increased the shrimp growth and survival in a recirculating system. - A pilot-scale constructed wetland unit, consisting of free water surface (FWS) and subsurface flow (SF) constructed wetlands arranged in series, was integrated into an outdoor recirculating aquaculture system (RAS) for culturing Pacific white shrimp (Litopenaeus vannamei). This study evaluated the performance of the wetland unit in treating the recirculating wastewater and examined the effect of improvement in water quality of the culture tank on the growth and survival of shrimp postlarvae. During an 80-day culture period, the wetland unit operated at a mean hydraulic loading rate of 0.3 m/day and effectively reduced the influent concentrations of 5-day biochemical oxygen demand (BOD{sub 5}, 24%), suspended solids (SS, 71%), chlorophyll a (chl-a, 88%), total ammonium (TAN, 57%), nitrite nitrogen (NO{sub 2}-N, 90%) and nitrate nitrogen (NO{sub 3}-N, 68%). Phosphate (PO{sub 4}-P) reduction was the least efficient (5.4%). The concentrations of SS, Chl-a, turbidity and NO{sub 3}-N in the culture tank water in RAS were significantly (P{<=}0.05) lower than those in a control aquaculture system (CAS) that simulated static pond culture without wetland treatment. However, no significant difference (P{<=}0.05) in BOD{sub 5}, TAN and NO{sub 2}-N was found between the two systems. At the end of the study, the harvest results showed that shrimp weight and survival rate in the RAS (3.8{+-}1.8 g/shrimp and 90%) significantly (P{<=}0.01) exceeded those in the CAS (2.3{+-}1.5 g/shrimp and 71%). This study concludes that constructed wetlands can improve the water quality and provide a good culture environment, consequently increasing the shrimp growth and survival without water exchange, in a recirculating system.

  12. Studies on the immunomodulatory effect of extract of Cyanodon dactylon in shrimp, Penaeus monodon, and its efficacy to protect the shrimp from white spot syndrome virus (WSSV).

    Science.gov (United States)

    Balasubramanian, G; Sarathi, M; Venkatesan, C; Thomas, John; Hameed, A S Sahul

    2008-12-01

    The present study investigates the protection of shrimp Penaeus monodon against white spot syndrome virus (WSSV) using antiviral plant extract derived from Cyanodon dactylon and the modulation of the shrimp non-specific immunity. To determine the antiviral activity, the shrimp were treated by both in vitro (intramuscular injection) and in vivo (orally with feed) methods at the concentration of 2mg per animal and 2% of the plant extract incorporated with commercially available artificial pellet feed, respectively. The antiviral activity of C. dactylon plant extract was confirmed by PCR, bioassay and Western blot analysis. In the present study, anti-WSSV activity of C. dactylon plant extract by in vivo and in vitro methods showed strong antiviral activity and the immunological parameters such as proPO, O(2)(-), NO, THC and clotting time were all significantly (Pprice, we believe that oral administration of C. dactylon plant extract along with the pellet feed is a potential prophylactic agent against WSSV infection of shrimp. PMID:18834943

  13. Grow-out of sandfish Holothuria scabra in ponds shows that co-culture with shrimp Litopenaeus stylirostris is not viable

    OpenAIRE

    Bell, J.; Agudo, N.; Purcell, S.; Blazer, P; Simutoga, M; Pham, Dominique; Della Patrona, Luc

    2007-01-01

    We examined the potential for producing the large numbers of sandfish (Holothuria scabra) needed for restocking programmes by co-culturing juveniles with the shrimp Litopenaeus stylirostris in earthen ponds. Our experiments in hapas within shrimp ponds were designed to detect any deleterious effects of sandfish on shrimp, and vice versa. These experiments showed that a high stocking density of juvenile sandfish had no significant effects on growth and survival of shrimp. However, survival and...

  14. CHINESE OF HUMANITY

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    A discussion of chinese curriculum of primary school under the background of new curriculum reform Mao xinjuan Feng haiying [Abstract] in recent years, Chinese learning received more and more attention by people article mainly from the national studies this course concepts, the curriculum reform of elementary school curriculum requirements and how to effective implementation of primary national studies course several aspects under the background of curriculum reform of Chinese primary curriculum the new school

  15. A physical model of the extreme mantis shrimp strike: kinematics and cavitation of Ninjabot

    International Nuclear Information System (INIS)

    To study the mechanical principles and fluid dynamics of ultrafast power-amplified systems, we built Ninjabot, a physical model of the extremely fast mantis shrimp (Stomatopoda). Ninjabot rotates a to-scale appendage within the environmental conditions and close to the kinematic range of mantis shrimp's rotating strike. Ninjabot is an adjustable mechanism that can repeatedly vary independent properties relevant to fast aquatic motions to help isolate their individual effects. Despite exceeding the kinematics of previously published biomimetic jumpers and reaching speeds in excess of 25 m s−1 at accelerations of 3.2 × 104 m s−2, Ninjabot can still be outstripped by the fastest mantis shrimp, Gonodactylus smithii, measured for the first time in this study. G. smithii reached 30 m s−1 at accelerations of 1.5 × 105 m s−2. While mantis shrimp produce cavitation upon impact with their prey, they do not cavitate during the forward portion of their strike despite their extreme speeds. In order to determine how closely to match Ninjabot and mantis shrimp kinematics to capture this cavitation behavior, we used Ninjabot to produce strikes of varying kinematics and to measure cavitation presence or absence. Using Akaike Information Criterion to compare statistical models that correlated cavitation with a variety of kinematic properties, we found that in rotating and accelerating biological conditions, cavitation inception is best explained only by maximum linear velocity. (paper)

  16. Effects of gamma radiation on the survival and growth of brine shrimp, Artemia salina

    International Nuclear Information System (INIS)

    To determine the effects of gamma radiation on the survival and growth of brine shrimp, nauplii and adults were exposed to different doses of radiation and maintained at different salinities and temperatures. The LD50 determination demonstrated that nauplii were thirty to forty times more sensitive than the adults and had a 25-day LD50 of 450 rads. Radiation and salinity reduced survival of adult female brine shrimp more than the males. The interaction of salinity, 5 and 50 ppt, temperature, 10, 20 and 300C, and radiation, 25 to 100 kilorads, decreased the survival of both male and female brine shrimp, with the males having the greater sensitivity. Brine shrimp nauplii irradiated with doses of 500 and 2,500 rads had accelerated growth and matured earlier than the controls. A test of the effect of crowding on growth showed that volume per individual was important, and a dose of 500 rads accelerated brine shrimp growth in all concentrations, with the greatest increase at 8 ml/nauplius

  17. Combination effects of radiation, packaging and N2 gas for shelf-life extension of shrimp

    International Nuclear Information System (INIS)

    To evaluate the influence of different parameters on the shelf-life of cooked shrimp experiments were made, introducing radiation in the shrimp (peeled and unpeeled), temperatures (deep frozen at -350C and chilled at 50C) and packing materials (EG-0 and CP) to reduce the oxidation effects, the experiment was performed under the addition of the nitrogen gas. The irradiation was done with gamma rays emitted by a cobalt-60 source of 5.55x1015Bp (150 KCi) at a dose rate of 6 kGy. The usual irradiation dose of 1.5 and 3 kGy were applied as well as a range of 0.5, 1, 2, 3, 4, 5, 6 and 8 kGy. The experiments confirmed that the radiation sensitivity of microorganism like coliforms and aerobic microorganisms and enterobacteriaceae is temperature depending. The peroxide value showed also to be temperature depending and in addition of the packaging material. The TNA-N value was not significantly influenced by the applied radiation doses. Shrimp irradiated at chilled conditions showed off-flavours at 1.5 kGy. Off-flavours could be detected at a dose of 3 kGy when the shrimp was irradiated at deep frozen condition. No difference was observed on the radiation effects on peeled and unpeeled shrimps

  18. A field survey of chemicals and biological products used in shrimp farming

    International Nuclear Information System (INIS)

    This study documented the use of chemicals and biological products in marine and brackish water shrimp farming in Thailand, the world's top producer of farmed shrimp. Interviews were conducted with 76 shrimp farmers in three major shrimp producing regions, the eastern Gulf coast, the southern Gulf coast and the Andaman coast area. Farmers in the study used on average 13 different chemicals and biological products. The most commonly used products were soil and water treatment products, pesticides and disinfectants. Farmers in the southern Gulf coast area used a larger number of products than farmers in the other two areas. In the study, the use of more than 290 different chemicals and biological products was documented. Many of the pesticides, disinfectants and antibiotics used by the farmers could have negative effects on the cultured shrimps, cause a risk for food safety, occupational health, and/or have negative effects on adjacent ecosystems. Manufacturers and retailers of the products often neglected to provide farmers with necessary information regarding active ingredient and relevant instructions for safe and efficient use

  19. Physiochemical Changes and Optimization of Phosphate-Treated Shrimp (Litopenaeus vannamei ) Using Response Surface Methodology.

    Science.gov (United States)

    Omar, Saiah Djebbour; Yang, Je-Eun; Oh, Sang-Cheol; Kim, Dae-Wook; Lee, Yang-Bong

    2016-03-01

    The objective of this study was to determine the factors responsible for the changed physiochemical properties of unpeeled shrimp treated in cold phosphate solution (2~4°C) with the intervention of 4 factors: phosphate concentration, dipping time, rotation speed, and volume of brine solution. Response surface analysis was used to characterize the effect of the phosphate treatment on shrimps by running 33 treatments for optimizing the experiment. For each treatment, phosphate amount, moisture content, and weight gain were measured. The results showed that phosphate concentration is the most important factor than other factors for facilitating phosphate penetration in the meat of the shrimp and for getting the best result. The optimum condition of phosphate-treated shrimp in this study was 110 to 120 min dipping time, 500 to 550 mL brine solution for 100 g shrimp sample, and 190 to 210 rpm agitation speed. The studied conditions can be applied in fisheries and other food industries for good phosphate treatments. PMID:27069905

  20. Problems related to the foreign exchange earnings of Surinam's shrimp industry

    Directory of Open Access Journals (Sweden)

    Pottier, D.

    1989-01-01

    Full Text Available The shrimp grounds of Surinam are actually fully exploited by some 136 trawlers of the Florida type, ranging from 100 to 130 GRT. Through export and import levies and fishing licence fees the country was able to obtain a small piece of the foreign currency cake. The Republic of Surinam has been looking for means to increase the benefits from its shrimp business. The alleged huge profits made by foreign vessels inspired the country to invest in a national shrimp fleet and man it with national crew (SUGAM. For reasons explained in the text Sugam has difficultes to operate with a gain. This paper will review Sugam's attempt to earn hard currency. An analysis is made of the foreign exchange earnings of Sugam and is compared with the results of a Korean company. The main conclusions are as follows : shrimp exploitation in a fully exploited Exclusive Economic Zone can only be brought to success by fishermen of proven ability. Each vessel should make trips lasting from 45 to 60 days and be some 280 days at sea per year. Surinam should also reconsider its present policy of shrimp purchasing.

  1. Replacement of fish meal with black soldier fly meal in practical diets for Pacific whiteleg shrimp Litopenaeus vannamei

    Science.gov (United States)

    Shrimp account for 15 percent of the total value of internationally traded fishery products, and currently are the largest single aquaculture commodity in value terms. The Pacific whiteleg shrimp (Litopenaeus vannamei) generated USD 11 billion from a production volume of 2.7 million metric tons (mm...

  2. 76 FR 23972 - Certain Frozen Warmwater Shrimp From Brazil, India, the People's Republic of China, Thailand, and...

    Science.gov (United States)

    2011-04-29

    ... (``Sunset'') Review, 75 FR 103 (January 4, 2010), and Frozen Warmwater Shrimp From Brazil, China, India... International Trade Administration Certain Frozen Warmwater Shrimp From Brazil, India, the People's Republic of...) from Brazil, India, the People's Republic of China (PRC), Thailand, and the Socialist Republic...

  3. 76 FR 23277 - Certain Frozen Warmwater Shrimp From Brazil, India, the People's Republic of China, Thailand, and...

    Science.gov (United States)

    2011-04-26

    ... Warmwater Shrimp From Brazil, 69 FR 76910 (December 23, 2004); Notice of Final Determination of Sales at... Canned Warmwater Shrimp From the People's Republic of China, 69 FR 70997 (December 8, 2004); Notice of... the People's Republic of China, 70 FR 5149 (February 1, 2005); Notice of Amended Final...

  4. 75 FR 27299 - Certain Frozen Warmwater Shrimp from Brazil, India, the People's Republic of China and Thailand...

    Science.gov (United States)

    2010-05-14

    ... and Antidumping Duty Order: Certain Frozen Warmwater Shrimp from Brazil, 70 FR 5143 (February 1, 2005... the People's Republic of China, 70 FR 5149 (February 1, 2005); and Notice of Amended Final... International Trade Administration Certain Frozen Warmwater Shrimp from Brazil, India, the People's Republic...

  5. Impact of solid shrimp pond waste materials on mangrove growth and mortality: a case study from Pak Phanang, Thailand

    NARCIS (Netherlands)

    Vaiphasa, C.; Boer, de W.F.; Skidmore, A.K.; Panitchart, S.; Vaiphasa, T.; Bamrongrugsa, N.; Santitamnont, P.

    2007-01-01

    One of the most serious threats to tropical mangrove ecosystems caused by shrimp farming activities is the poor management of pond wastematerials.We hypothesise thatmangroves can tolerate chemical residues discharged from shrimp farms and can be used as biofilters, but the capability of mangroves to

  6. Determination of oxytetracycline residue in shrimp using a portable time-resolved analyzer and HPLC-MS/MS validation

    Science.gov (United States)

    Oxytetracycline (OTC) is the most prominent antibiotic in shrimp aquaculture. In this work, OTC residue in shrimp muscle is determined using a portable analyzer built in this laboratory based on europium-sensitized luminescence (ESL). First, OTC is extracted in McIlvaine buffer at pH 4.0 with 0.1 ...

  7. Water pollution by intensive brackish shrimp farming in south-east Vietnam: Causes and options for control

    NARCIS (Netherlands)

    Pham Thi Ahn,; Kroeze, C.; Bush, S.R.; Mol, A.P.J.

    2010-01-01

    This paper focuses on both the environmental impact of intensive shrimp farming in the coastal region of Vietnam and the identification of options for cleaner production. We investigated water pollution, sediment contamination and the spread of diseases related to shrimp farming in the Can Gio distr

  8. Current status of genetics and genomics of reared penaeid shrimp: information relevant to access and benefit sharing.

    Science.gov (United States)

    Andriantahina, Farafidy; Liu, Xiaolin; Feng, Tingting; Xiang, Jianhai

    2013-08-01

    At present, research and progress in shrimp genomics and genetics show significant developments. Shrimp genetics and genomics also show immense potential for an increased production in a way that meets shrimp culture progress goals for the third millennium. This review article aims to provide an overview of its current status and future direction, discusses questions that need focused research to address them, and summarizes areas where genetics and genomics knowledge can make a positive difference to shrimp culture sustainability. Sustainable progress of penaeid shrimps will depend upon feasible solutions for environmental, research, economic, consumer problems, proper development, and planning policy enforcement. It is recommended that increased funding for biotechnology research and progress be directed to expand worldwide commercial shrimp culture and address environmental and public health issues. For any researcher or shrimp company member who has attempted to or whom would like to thoroughly search the literature to gain a complete understanding of the current state of shrimp genetics and genomics, this publication will be an invaluable source of reference materials, some of which is reported here for the first time. PMID:23529408

  9. Circularly Polarized Light as a Communication Signal in Mantis Shrimps.

    Science.gov (United States)

    Gagnon, Yakir Luc; Templin, Rachel Marie; How, Martin John; Marshall, N Justin

    2015-12-01

    Animals that communicate using conspicuous body patterns face a trade-off between desired detection by intended receivers and undesired detection from eavesdropping predators, prey, rivals, or parasites. In some cases, this trade-off favors the evolution of signals that are both hidden from predators and visible to conspecifics. Animals may produce covert signals using a property of light that is invisible to those that they wish to evade, allowing them to hide in plain sight (e.g., dragonfish can see their own, otherwise rare, red bioluminescence). The use of the polarization of light is a good example of a potentially covert communication channel, as very few vertebrates are known to use polarization for object-based vision. However, even these patterns are vulnerable to eavesdroppers, as sensitivity to the linearly polarized component of light is widespread among invertebrates due to their intrinsically polarization sensitive photoreceptors. Stomatopod crustaceans appear to have gone one step further in this arms race and have evolved a sensitivity to the circular polarization of light, along with body patterns producing it. However, to date we have no direct evidence that any of these marine crustaceans use this modality to communicate with conspecifics. We therefore investigated circular polarization vision of the mantis shrimp Gonodactylaceus falcatus and demonstrate that (1) the species produces strongly circularly polarized body patterns, (2) they discriminate the circular polarization of light, and (3) that they use circular polarization information to avoid occupied burrows when seeking a refuge. PMID:26585281

  10. Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond.

    Science.gov (United States)

    Sheu, Shih-Yi; Lin, Kuan-Yin; Chou, Jui-Hsing; Chang, Poh-Shing; Arun, A B; Young, Chiu-Chung; Chen, Wen-Ming

    2007-05-01

    A yellow-coloured, aerobic, Gram-negative, rod-shaped bacterial strain, designated B-I(T), was isolated from the water of a shrimp (Litopenaeus vannamei) mariculture pond in Taiwan. No species with a validly published name showed 16S rRNA gene sequence similarity of more than 96.5 % to this novel isolate. The highest sequence similarities displayed by strain B-I(T) (93.2-96.1 %) were to members of the genus Tenacibaculum. The phenotypic properties of this organism were consistent with its classification in the genus Tenacibaculum. The novel isolate could be distinguished from all Tenacibaculum species by several phenotypic characteristics. The major fatty acids were iso-C(15 : 0) (22 %), summed feature 3 (C(16 : 1)omega7c and/or iso-C(15 : 0) 2-OH; 21.3 %), iso-C(17 : 0) 3-OH (12.7 %) and iso-C(15 : 1) (8.7 %). The G+C content of the genomic DNA was 35.2 mol%. Hence, genotypic and phenotypic data demonstrate that strain B-I(T) should be classified within a novel species in the genus Tenacibaculum, for which the name Tenacibaculum litopenaei sp. nov. is proposed. The type strain is B-I(T) (=BCRC 17590(T)=LMG 23706(T)). PMID:17473274

  11. Chinese Foods; Teacher's Handbook.

    Science.gov (United States)

    Huang, Joe, Ed.

    Different styles of Chinese cooking, traditional food items, cooking utensils, serving techniques, and the nutritional value of Chinese cooking are described in this teaching guide. Lesson plans for the preparation of simple dishes are presented. Recipes, a shopping guide to San Francisco's Chinatown, a guide to sources of supplies, and a…

  12. Confucius Teaching Chinese Abroad

    Institute of Scientific and Technical Information of China (English)

    TANG YUANKAI

    2010-01-01

    @@ On December 1, 2009, the Confucius Institute in Lyon, France, held a plaque-unveiling ceremony. Thomas Boutonnet, a Frenchman who has studied Chinese for 10 years in France and China and who is also an institute supervisor, said the institute would offer courses in Chinese language and culture covering legal, wade and cultural fields.

  13. FROM THE CHINESE PRESS

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    Equality and harmony are mainstream in Chinese marriage. The conclusion was made by a systematic investigation in 1996 on love and marriage relations between couples in Shanghai, Harbin, Guangdong, Gansu and other regions. Six thousand couples were surveyed in a multi-period, separated level probability sampling; the research was conducted by the study group, "Marriage quality during the period of Chinese social

  14. Say That in Chinese

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Demand for Chinese language learning is fueling all aspects of the market, most notably the textbook publication industry Alarge-scale series of Chinese lan-guage textbooks are to be pub-lished in the coming years jointly by the China International Publi

  15. On Developing Business Chinese.

    Science.gov (United States)

    Hong, Wei

    1996-01-01

    Examines the significance of foreign languages for business, particularly Business Chinese, in the 1990s; its curriculum requirements; and the impact of business languages on international business. The article proposes a developmental plan for Business Chinese at the college level including goals, course materials, learning activities, and…

  16. Chinese by Choice

    Science.gov (United States)

    Beem, Kate

    2008-01-01

    A 2004 College Board survey revealed that school districts around America wanted to offer Chinese, but finding qualified teachers was a problem, says Selena Cantor, director of Chinese Language and Culture Initiatives for the College Board. So last year, a new College Board program brought guest teachers from China to school districts in 31…

  17. Equilibria of Chinese Auctions

    DEFF Research Database (Denmark)

    Branzei, Simina; Forero, Clara; Larson, Kate;

    Chinese auctions are a combination between a raffle and an auction and are held in practice at charity events or festivals. In a Chinese auction, multiple players compete for several items by buying tickets, which can be used to win the items. In front of each item there is a basket, and the play...

  18. Technical Efficiency of Shrimp Farming in Andhra Pradesh: Estimation and Implications

    Directory of Open Access Journals (Sweden)

    I. Sivaraman

    2015-04-01

    Full Text Available Shrimp farming is a key subsector of Indian aquaculture which has seen a remarkable growth in the past decades and has a tremendous potential in future. The present study analyzes the technical efficiency of the shrimp famers of East Godavari district of Andhra Pradesh using the Stochastic Production Frontier Function with the technical inefficiency effects. The estimates mean technical efficiency of the farmers was 93.06 % which means the farmers operate at 6.94 % below the production frontier production. Age, education, experience of the farmers and their membership status in farmers associations and societies were found to have a significant effect on the technical efficiency. The variation in the technical efficiency also confirms the differences in the extent of adoption of the shrimp farming technology among the farmers. Proper technical training opportunities could facilitate the farmers to adopt the improved technologies to increase their farm productivity.

  19. Antimutagenicity and Antiproliferative Studies of Lipidic Extracts from White Shrimp (Litopenaeus vannamei)

    Science.gov (United States)

    Wilson-Sanchez, Griselda; Moreno-Félix, Carolina; Velazquez, Carlos; Plascencia-Jatomea, Maribel; Acosta, Anita; Machi-Lara, Lorena; Aldana-Madrid, María-Lourdes; Ezquerra-Brauer, Josafat-Marina; Robles-Zepeda, Ramón; Burgos-Hernandez, Armando

    2010-01-01

    An organic extract from fresh shrimp (Litopenaeus vannamei) was studied for antimutagenic and antiproliferative properties using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9) and a cancer cell line (B-cell lymphoma), respectively. Shrimp extract was sequentially fractionated by thin layer chromatography (TLC) and each fraction was tested for antimutagenic and antiproliferative activities. Crude organic extracts obtained from shrimp reduced the number of revertants caused by aflatoxina B1, showing a dose-response type of relationship. Sequential TLC fractionation of the active extracts produced several antimutagenic and/or antiproliferative fractions. These results suggested that the lipid fraction of the tested species contained compounds with chemoprotective properties that reduce the mutagenicity of AFB1 and proliferation of a cancer cell line. PMID:21139845

  20. Antimutagenicity and Antiproliferative Studies of Lipidic Extracts from White Shrimp (Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Carolina Moreno-Félix

    2010-11-01

    Full Text Available An organic extract from fresh shrimp (Litopenaeus vannamei was studied for antimutagenic and antiproliferative properties using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9 and a cancer cell line (B-cell lymphoma, respectively. Shrimp extract was sequentially fractionated by thin layer chromatography (TLC and each fraction was tested for antimutagenic and antiproliferative activities. Crude organic extracts obtained from shrimp reduced the number of revertants caused by aflatoxina B1, showing a dose-response type of relationship. Sequential TLC fractionation of the active extracts produced several antimutagenic and/or antiproliferative fractions. These results suggested that the lipid fraction of the tested species contained compounds with chemoprotective properties that reduce the mutagenicity of AFB1 and proliferation of a cancer cell line.

  1. Arsenic speciation in shrimp and mussel from the Mid-Atlantic hydrothermal vents

    DEFF Research Database (Denmark)

    Larsen, Erik Huusfeldt; Quetel, C. R.; Munoz, R.; FialaMedioni, A.; Donard, O. F. X.

    Specimens of shrimp (Rimicaris exoculata) and mussel (Bathymodiolus puteoserpentis) were collected 3500 m below the ocean surface at the hydrothermal vents of the mid-Atlantic Ridge (TAG and Snake Pit sites, respectively). Arsenic, a potentially toxic element, is among the substances emitted by the...... hydrothermal vents. The hydrothermal vent shrimp, which are known to be a primary consumer of the primary producing chemolithoautotrophic bacteria, contained arsenic at 13 mu g g(-1) almost exclusively as arsenobetaine (AsB). Arsenic was present in the soft:issues of the mussel at 40 mu g g(-1) and the major...... of arsenic species found in the shrimp and mussel species in the deep-sea is similar to that found in their counterparts from the ocean surface. It is concluded that the autotrophic bacteria of the hydrothermal vent ecosystem and the symbiotic bacteria harboured in the mussel species are responsible...

  2. Effects of pesticides on DNA and protein of shrimp larvae Litopenaeus stylirostris of the California Gulf.

    Science.gov (United States)

    Galindo Reyes, J Guillermo; Leyva, Nancy R; Millan, Olivia A; Lazcano, Guadalupe A

    2002-10-01

    Recently, diverse pathologies and massive mortalities have been presented in shrimp hatcheries located along the California Gulf; therefore, toxic responses of shrimp larvae were used as biomarkers of pesticide pollution, because in this region intensive agriculture is practiced. Shrimp larvae were exposed to DDT, azinphosmethyl, permethrine, parathion, chlorpyrifos, malathion, endosulfan, and carbaryl, in order to determine LC50, DNA adducts and/or breaks, and total protein in larvae. The results indicate reductions in protein and DNA in larvae exposed to these pesticides, and in those exposed to DDT, breaks and/or adducts were registered. It is possible that pesticide pollution is a cause of these problems, because reduction in protein indicates a decrease in larvae growth rate and DNA breaks or adducts have been related to pathologies and carcinogenesis in many aquatic organisms. PMID:12568452

  3. Metal content of the gulf of California blue shrimp Litopenaeus stylirostris (Stimpson).

    Science.gov (United States)

    Frías-Espericueta, M G; Izaguirre-Fierro, G; Valenzuela-Quiñonez, F; Osuna-López, J I; Voltolina, D; López-López, G; Muy-Rangel, M D; Rubio-Castro, W

    2007-08-01

    The blue shrimp Litopenaeus stylirostris is the main target species of the Gulf of California shrimping fleet, and its heavy metal content might be of concern for human health because of the increasing contamination of the Gulf. The Cd content of shrimp caught by commercial trawlers ranged from 0.38 to 1.05 microg/g and the mean value was significantly lower in the northern fishing grounds. Pb ranged from 3.19 to 9.59 microg/g and was significantly higher in the northern area. There were no significant geographic difference in the case of Cu and Zn (respective means = 25.4 and 57.8 microg/g). The mean values of all metals show that none is of particular concern for human health. PMID:17639322

  4. Detection of hepatopancreatic parvovirus (HPV) in wild shrimp from India by nested polymerase chain reaction (PCR).

    Science.gov (United States)

    Manjanaik, B; Umesha, K R; Karunasagar, Indrani; Karunasagar, Iddya

    2005-02-28

    The prevalence of hepatopancreatic parvovirus (HPV) in wild penaeid shrimp samples from India was studied by nested polymerase chain reaction (PCR) using primers designed in our laboratory. The virus could be detected in 9 out of 119 samples by non-nested PCR. However, by nested PCR 69 out of 119 samples were positive. The PCR results were confirmed by hybridization with digoxigenin-labelled DNA probe. Shrimp species positive by non-nested PCR included Penaeus monodon, Penaeus indicus and Penaeus semisulcatus and by nested PCR Parapenaeopsis stylifera, Penaeus japonicus, Metapenaeus monoceros, M. affinis, M. elegans, M. dobsoni, M. ensis and Solenocera choprai. This is the first report on the prevalence of HPV in captured wild shrimp from India. PMID:15819441

  5. Prevalence and characterization of Vibrio cholerae isolated from shrimp products imported into Denmark

    DEFF Research Database (Denmark)

    Dalsgaard, A.; Bjergskov, T.; Jeppesen, V.F.;

    1996-01-01

    A total of 3,555 metric tonnes of warm water shrimp were imported into Denmark from December 1994 to July 1995. V. cholerae O1 was not detected in any of the 748 samples analyzed. Non-Ol V. cholerae was found in a single (0.1%) cooked frozen shrimp product and in five (0.7%) raw frozen products...... contained plasmids or genes encoding cholera toxin (CT) or heat-stable enterotoxin (NAG-ST), The absence of V. cholerae O1 and the low number of samples containing CT and NAG-ST negative non-Ol strains in imported shrimp suggest that I! cholerae in such products may not constitute a public health problem....

  6. Impact of metal pollution on shrimp Crangon affinis by NMR-based metabolomics.

    Science.gov (United States)

    Ji, Chenglong; Yu, Deliang; Wang, Qing; Li, Fei; Zhao, Jianmin; Wu, Huifeng

    2016-05-15

    Both cadmium and arsenic are the important metal/metalloid pollutants in the Bohai Sea. In this work, we sampled the dominant species, shrimp Crangon affinis, from three sites, the Middle of the Bohai Sea (MBS), the Yellow River Estuary (YRE) and the Laizhou Bay (LZB) along the Bohai Sea. The concentrations of metals/metalloids in shrimps C. affinis indicated that the YRE site was polluted by Cd and Pb, while the LZB site was contaminated by As. The metabolic differences between shrimps C. affinis from the reference site (MBS) and metal-pollution sites (YRE and LZB) were characterized using NMR-based metabolomics. Results indicated that the metal pollutions in YRE and LZB induced disturbances in osmotic regulation and energy metabolism via different metabolic pathways. In addition, a combination of alanine and arginine might be the biomarker of Cd contamination, while BCAAs and tyrosine could be the biomarkers of arsenic contamination in C. affinis. PMID:26920426

  7. The effect of simulated gastrointestinal digestion on shrimp Penaeus vannamei allergenicity

    Institute of Scientific and Technical Information of China (English)

    GUO Yongchao; LI Zhenxing; LIN Hong

    2009-01-01

    Pen a 1 is a major shrimp allergen that induces an allergy. The aim of this study is to assess the effect of simulated gastrointestinal fluids on the allergenicity of Pen a 1. Purified Pen a 1 from shrimp (Penaeus vannamei) was subjected to digestion in SGF (simulated gastric fluid) and SIF (simulated intestinal fluid) for a set time. The allergenicity of digestive Pen a 1 was analyzed by immunoblotting and Ci-ELISA, using pool sera from patients with shrimp specific IgE. The results showed that Pen a 1 exhibited a decrease in allergenicity with increasing digestion time in the SGF and SIF. However, Pen a l exhibited strong resistance to digestive fluids, and all yielded fragments (33 kD, 23 kD, and 14 kD) showed allergic activity. Therefore, anti-digestion may be an important factor for Pen a 1 to induce an allergy.

  8. Analysis of production and environmental effects of Nile tilapia and white shrimp culture in Thailand

    DEFF Research Database (Denmark)

    Ferreira, J.G.; Falconer, L.; Kittiwanich, J.;

    2015-01-01

    Two case studies from Southeast Asia are used to analyse production, environmental effects, and economic optimisation of Nile tilapia (Oreochromis niloticus) and white shrimp (Penaeus vannamei) pond culture. A projection of these data is made for the whole of Thailand. The results are analysed on a...... regional scale based on site selection using multi-criteria evaluation (MCE). Farm-scale culture was simulated for (i) tilapia monoculture in Chiang Rai; (ii) shrimp monoculture in Chanthaburi; and (iii) Integrated Multi-Trophic Aquaculture (IMTA) of tilapia and shrimp in Chon Buri. Together, these...... provinces produced 17,500 tonnes of tilapia in 2012, with a significant proportion exported to North America and Europe. Growth models for both species were developed, calibrated, and validated, and used to simulate population dynamics of cultivated animals, and sediment diagenesis and eutrophication in...

  9. Influence of Probiotics on the Growth and Digestive Enzyme Activity of White Pacific Shrimp (Litopenaeus vannamei)

    Institute of Scientific and Technical Information of China (English)

    G(o)mez R. Geovanny D.; MA Shen

    2008-01-01

    The influence of Bacillus probiotics on the digestive enzyme activity and the growth of Litopenaeus vannamei were de-termined in this study. The shrimp was treated with five percentages (1.5, 3.0, 4.5, 6.0 and 7.5) of probiotics (Bacillus spp.) supple-mented to the feed and cultured for 45d. The growth measured as the weight gain at the end of culturing was significantly (P<0.05) higher in probiotic-treated shrimps than that of the control (without receiving probiotics). Activities of protease and amylase, two digestive enzymes of the midgut gland and the intestine were significantly (P<0.05) higher in probiotic-treated shrimp than in the control.

  10. Arsenic speciation in shrimp and mussel from the Mid-Atlantic hydrothermal vents

    DEFF Research Database (Denmark)

    Larsen, Erik Huusfeldt; Quetel, C. R.; Munoz, R.;

    1997-01-01

    Specimens of shrimp (Rimicaris exoculata) and mussel (Bathymodiolus puteoserpentis) were collected 3500 m below the ocean surface at the hydrothermal vents of the mid-Atlantic Ridge (TAG and Snake Pit sites, respectively). Arsenic, a potentially toxic element, is among the substances emitted by the...... hydrothermal vents. The hydrothermal vent shrimp, which are known to be a primary consumer of the primary producing chemolithoautotrophic bacteria, contained arsenic at 13 mu g g(-1) almost exclusively as arsenobetaine (AsB). Arsenic was present in the soft:issues of the mussel at 40 mu g g(-1) and the major...... of arsenic species found in the shrimp and mussel species in the deep-sea is similar to that found in their counterparts from the ocean surface. It is concluded that the autotrophic bacteria of the hydrothermal vent ecosystem and the symbiotic bacteria harboured in the mussel species are responsible...

  11. Modern Chinese History Studies

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Famous Foreign Scholars' Lecture Tours in China Around the May Fourth Movement and Changes in Chinese Intellectual Circles From 1919 to 1924, John Dewey and four other famous foreign scholars came to China on lecture tours. These tours were great cultural undertakings to spread Western learning to the East after the First World War. The lectures these schol- ars gave helped to deepen the thoughts of Chinese people, and at the same time encouraged the diversification and evolution of Chinese intellectual circles. Firstly, the lectures hastened the birth of a contemporary Chinese wave of reflection on mo- dernity, and provided a basis for the theoretical views and cultural appeals of Liang Qichao and other members of the socalled "Orient Culture Faction," thereby increasing the tension intrinsic to the development of the New Culture Movement and to the expansion of intellectual horizons in Chinese intellectual circles.

  12. Chinese Companies in Switzerland

    Directory of Open Access Journals (Sweden)

    Esther Kessler

    2014-10-01

    Full Text Available In recent years, some of China’s leading firms have made headlines with their European expansion, by either opening new facilities or by acquiring or merging with significant enterprises in Europe. The goal of this paper is to contribute to the existing literature by examining Chinese enterprises expanding into Switzerland. The study also allows some conclusions for Chinese companies entering Central and Eastern Europe. We analyze via interviews the motivations of Chinese companies to expand into Switzerland as well as their behavior and the impediments in their internationalization process. Our findings show that Chinese companies fail to take advantage of certain benefits of western economies (such as open information and stable rule of law. To move forward efficiently, they should develop competence in dealing systematically with readily available market information, building professional networks that recognize a separation between business life and personal life, and managing their Chinese and foreign employees in the foreign cultural environment.

  13. On Chinese Parody Translation

    Institute of Scientific and Technical Information of China (English)

    熊俊

    2013-01-01

    Chinese parody, as a traditional figure of speech, has captured more and more attention from scholars. The researches conducted up to date are inadequate in theorizing and exploring its translation. This paper, based on the comparative data analysis of Chinese parody translation examples in different types of texts, attempts to probe into the means about how to achieve the clos⁃est function equivalence in rendering Chinese parody under the guidance of Sociosemiotic Approach. It is found that the nature of Chinese parody translation is to achieve the closest natural equivalence or similarity in expressive function, informative func⁃tion, vocative function and aesthetic function in its equivalents in English. And it is suggested that borrowing, imitating, para⁃phrasing and adapting are effective strategies in translating Chinese parody.

  14. Effects of predation on diel activity and habitat use of the coral-reef shrimp Cinetorhynchus hendersoni (Rhynchocinetidae)

    Science.gov (United States)

    Ory, Nicolas C.; Dudgeon, David; Duprey, Nicolas; Thiel, Martin

    2014-09-01

    Nonlethal effects of predators on prey behaviour are still poorly understood, although they may have cascading effects through food webs. Underwater observations and experiments were conducted on a shallow fringing coral reef in Malaysia to examine whether predation risks affect diel activity, habitat use, and survival of the rhynchocinetid shrimp Cinetorhynchus hendersoni. The study site was within a protected area where predatory fish were abundant. Visual surveys and tethering experiments were conducted in April-May 2010 to compare the abundance of shrimps and predatory fishes and the relative predation intensity on shrimps during day and night. Shrimps were not seen during the day but came out of refuges at night, when the risk of being eaten was reduced. Shrimp preferences for substrata of different complexities and types were examined at night when they could be seen on the reef; complex substrata were preferred, while simple substrata were avoided. Shrimps were abundant on high-complexity columnar-foliate Porites rus, but tended to make little use of branching Acropora spp. Subsequent tethering experiments, conducted during daytime in June 2013, compared the relative mortality of shrimps on simple (sand-rubble, massive Porites spp.) and complex ( P. rus, branching Acropora spp.) substrata under different predation risk scenarios (i.e., different tether lengths and exposure durations). The mortality of shrimps with short tethers (high risk) was high on all substrata while, under low and intermediate predation risks (long tethers), shrimp mortality was reduced on complex corals relative to that on sand-rubble or massive Porites spp. Overall, mortality was lowest on P. rus. Our study indicates that predation risks constrain shrimp activity and habitat choice, forcing them to hide deep inside complex substrata during the day. Such behavioural responses to predation risks and their consequences for the trophic role of invertebrate mesoconsumers warrant further

  15. BAC end sequencing of Pacific white shrimp Litopenaeus vannamei: a glimpse into the genome of Penaeid shrimp

    Science.gov (United States)

    Zhao, Cui; Zhang, Xiaojun; Liu, Chengzhang; Huan, Pin; Li, Fuhua; Xiang, Jianhai; Huang, Chao

    2012-05-01

    Little is known about the genome of Pacific white shrimp ( Litopenaeus vannamei). To address this, we conducted BAC (bacterial artificial chromosome) end sequencing of L. vannamei. We selected and sequenced 7 812 BAC clones from the BAC library LvHE from the two ends of the inserts by Sanger sequencing. After trimming and quality filtering, 11 279 BAC end sequences (BESs) including 4 609 pairedends BESs were obtained. The total length of the BESs was 4 340 753 bp, representing 0.18% of the L. vannamei haploid genome. The lengths of the BESs ranged from 100 bp to 660 bp with an average length of 385 bp. Analysis of the BESs indicated that the L. vannamei genome is AT-rich and that the primary repeats patterns were simple sequence repeats (SSRs) and low complexity sequences. Dinucleotide and hexanucleotide repeats were the most common SSR types in the BESs. The most abundant transposable element was gypsy, which may contribute to the generation of the large genome size of L. vannamei. We successfully annotated 4 519 BESs by BLAST searching, including genes involved in immunity and sex determination. Our results provide an important resource for functional gene studies, map construction and integration, and complete genome assembly for this species.

  16. Dual oxidases participate in the regulation of intestinal microbiotic homeostasis in the kuruma shrimp Marsupenaeus japonicus.

    Science.gov (United States)

    Yang, Hui-Ting; Yang, Ming-Chong; Sun, Jie-Jie; Shi, Xiu-Zhen; Zhao, Xiao-Fan; Wang, Jin-Xing

    2016-06-01

    The metazoan gut lumen harbors numerous microbial communities. Tolerance for high bacterial counts and maintenance of microbiota homeostasis remain insufficiently studied. In this study, we identified a novel dual oxidase (MjDUOX2) involved in reactive oxygen species (ROS) production in the kuruma shrimp Marsupenaeus japonicus. MjDUOX2 is a transmembrane protein with an N-signal peptide region (19 aa) and a peroxidase homology domain (PHD, 554 aa) in the extracellular region; seven transmembrane regions; and three EF (calcium-binding region) domains (110 aa), a FAD-binding domain (104 aa), and a NAD-binding domain (156 aa) in the intracellular region. The novel MjDUOX2 exhibits a relatively low similarity (26.84% identity) to a previously reported DUOX in the shrimp (designated as MjDUOX1). The mRNA of MjDUOXs was widely distributed in the hemocytes, heart, hepatopancreas, gills, stomach, and intestine. Oral infection of the shrimp with pathogenic bacteria upregulated the mRNA expression of MjDUOXs and increased the ROS level in the intestine. However, High ROS level could inhibit the expression of MjDUOXs in shrimp after Vibrio anguillarum infection. Knockdown of MjDUOXs by RNA interference (RNAi) decreased the ROS level, increased the bacterial count in the intestine, and decreased the survival rate of the MjDUOX-RNAi shrimp infected with V. anguillarum. These results suggest that MjDUOXs play an important role for microbiota homeostasis in intestine of shrimp. PMID:26845611

  17. Supplement and Consumption of Dissolved Oxygen and Their Seasonal Variations in Shrimp Pond

    Institute of Scientific and Technical Information of China (English)

    孙耀; 张淑芳; 陈聚法; 宋云利

    2001-01-01

    On the basis of the research of DO budget or kinetics in shrimp pond, the main influence process of DO and its seasonal variations are quantitatively described through redividing the budget process and modifying the quantitative method of the process. The percentages of oxygen demand of various processes in the total oxygen demand are different in shrimp cultivation seasons. It is showed that the dissolved oxygen demand of mini-organisms is the major affected factor of DO in this environment and approximately accounts for 64.1~74.1% of the total oxygen demand. In the early period of shrimp culture, the dissolved oxygen demand of allotrophic bacteria degrading organic matters is much lower than that of phytoplankton respiration. But in the midterrn and later period, it is about 50% of the total oxygen demand because of the higher water temperature and more serious self-pollution. The dissolved oxygen demand of sediment is lower and just 19.1~28.8%, while the percentage of shrimp oxygen demand is lower. The effect of phytoplankton on DO in shrimp culturing water has dualism. One is the oxygen producing process of photosynthesis and the other is the oxygen consumption process of respiration. It is estimated that the dissolved oxygen demand of phytoplankton respiration is approximately one-fifth of the oxygen produced by photosynthesis under normal illumination conditions. The dissolved oxygen demand of al lotrophic bacteria degrading organic matters and the total oxygen demand of sediment increase 4 times and 1.7 times respectively from the early period to the midterm and later period.Obviously, the DO of culturing water can be also greatly improved by controlling the selfpollution of organic matters during shrimp culture.

  18. Sediment load and bioconcentration of heavy metals by shrimp (Peaneus notalis) from Epe Lagoon, Nigeria.

    Science.gov (United States)

    Olusegun, Ayejuyo O; Olalekan, Omojole M; Oladipo, Ojoniyi J

    2010-04-01

    In many developing countries, surface waters, especially rivers and lagoons, have become deposition sites for all sorts of wastes. Three sampling sites located trigonally on Epe lagoon, Lagos, Nigeria were identified, and water, sediment, and commonly farmed shrimp, Peaneus notalis, were sampled and assessed for heavy metal concentrations. Zn, Pb, Ni, Cu, Cr, Cd, and Mn were determined in the aqua regia digests of the samples by means of Perkin Elmer AAnalyst 200 atomic absorption spectrophotometer. Heavy metals occurred above detection limits at all sites. The highest load of these trace elements was found in sediment samples followed by the water samples. The levels observed in the shrimp samples, however, were much lower than WHO levels. In the distribution, zinc was the highest metal found in the samples ranging from 17.94 +/- 2.60 mg/L in water to 37.85 +/- 9.35 microg/g in sediments. The least was Cd 0.03 +/- 0.01 mg/L in water and 0.28 +/- 0.04 microg/g in sediments. Lead was the most widely varied metal in shrimp with a 84.38% coefficient of variation. Apart from Cr, the metals appeared to be fairly and evenly distributed in the sediment, with coefficient of variation ranging from 14.29% (Cd) to 24.76% (Mn) The variations were between 14.49% (Zn) and 66.67% (Cr) in the water samples. The paired t test carried out between water and sediment, water and shrimp, and shrimp and sediment was significant at p = 0.05. Three-factor analysis of variance did not reveal any significant difference in metal burdens among the trigonally located sites under investigation. Shrimp samples showed evidence of bioaccumulation but are still below internationally established levels. PMID:19267208

  19. Selection of proteolytic bacteria with ability to inhibit Vibrio harveyi during white shrimp (Litopenaeus vannamei cultivation

    Directory of Open Access Journals (Sweden)

    Suntinanalert, P.

    2007-03-01

    Full Text Available Five isolates of bacteria with high proteolytic activity, isolated from water samples of intensive shrimp ponds in southern Thailand, were selected to test for the ability to control the shrimp pathogen Vibrioharveyi. 70 μl of each culture broth were investigated for their ability to inhibit V. harveyi using an agar well diffusion test but only one isolate W3 gave a reasonable sized inhibition zone of 21.62 mm. This zone wassimilar to that of oxolinic acid (2 μg and sulfamethoxazole (25 μg. The W3 isolate was identified as Pseudomonas sp. Shrimp cultivation in aquaria was conducted to investigate the inhibition of V. harveyi bythe isolate W3. The experiment consisted of a treatment of the shrimp culture with an inoculum of the isolate W3 and V. harveyi (biocontrol set, a positive control set (only inoculation of V. harveyi and a negativecontrol set as without inoculation. No mortality was found in the negative control. Shrimp mortality in the biocontrol set (33% was lower than that in the positive control set (40%; however, it showed no significantdifference (p>0.05. The average numbers of V. harveyi over 12 days of the biocontrol set were lower than those in the positive control set by about 1 log cycle although the numbers were not significantly different(p>0.05. The shrimp growth rate at day 32 of cultivation was in order of the biocontrol treatment (10.17% > the negative control treatment (9.44% > the positive control set (9.28%, but no significant difference (p>0.05 was observed among treatments.

  20. Socio-Economic Structure of the Deep Water Pink Shrimp Fisheries

    Directory of Open Access Journals (Sweden)

    G. Gungor

    2007-09-01

    Full Text Available This research was done to be determined the socio-economic structure of shrimp fishery in the Marmara sea. The main material of this research is the original data which has been obtained by face-to-face interviewing with the vessel owners via using questionnaires during 2003-2004 shrimp fishery season. In the questionnaires the fallowing data has been taken places which are given respectively : About vessels and fishery activities: Length of vessels, engine power, construction material, fishery equipments, vessel construction place and date, the number of crew in per vessel, quantity of fishing shrimps according to the maths, fishing periods, average selling price, marketing channels. About social-economic structures vessels‟ owners: Vessels‟ owners age, beginning year of shrimp fishing, education, number and ages of family members, professions cost of shrimp fishing and gross revenues and etc., of fathers, having social security and etc. It was utilized as a demographic indicator for some fishery community‟s characteristics. The vessels which are taken into sample were determined by using “Strafied Random Sampling Method”. There were 63 vessels calculated by using formula. Vessels were classified among three groups. In the first group it was accepted that the vessels which are smaller than 10 m were called as “small”, 10-15 m were “medium” and longer than 15 m were “big”. In the first group it was calculated and then into consideration for interviewing 27 vessels, in the second group 29 vessels, and into last group 7 vessels. All data were analyzed by these three groups comparatively. The research data obtained were analyzed by using Microsoft word and Excel programs. Consequently, the detailed data were summarized in the table by absolute and rational values. According to the research findings; although many problems have to be solved related with shrimp fisheries most the families could get adequate level of income

  1. Histopathological and biochemical evidence of hepatopancreatic toxicity caused by cadmium in white shrimp, Palaemonetes argentinus.

    Science.gov (United States)

    Chiodi Boudet, L N; Polizzi, P; Romero, M B; Robles, A; Marcovecchio, J E; Gerpe, M S

    2015-03-01

    Cadmium (Cd) is one of the most common pollutants in the environment and induces a range of tissue changes or damages and organ dysfunction. The histopathological effects of Cd and lipid peroxidation (LPO) on hepatopancreas of the freshwater shrimp, Palaemonetes argentinus, were studied. Shrimp were obtained from two lagoons with contrasting environmental quality, De los Padres (LP, impacted site) and Nahuel Rucá (NR, reference site), and were exposed to 3.06 and 12.24µgCdL(-1) for 3, 7, 10 and 15 days. The health status of both populations was also evaluated by histological analysis of control individuals. After exposure, shrimp were transferred to clean water for 28 days to evaluate the recuperation capacity of hepatopancreas. Control shrimp from NR exhibited a normal hepatopancreas structure; unlike control shrimp from LP which showed several alterations. These results were attributed to the different environmental quality of lagoons. The exposure to Cd resulted in several alterations in the histological structure of the hepatopancreas of both populations. The observed alterations included haemocytic and connective infiltrations in the intertubular space, erosioned microvilli, ripple of basal lamina, atrophied epithelium and necrosis, however, the latter was only observed in shrimp from LP. The exposure also caused an increase of LPO levels in both populations. P. argentinus was able to repair the hepatopancreas structure from the damage caused by Cd, evidenced by the histopathological results and LPO levels. Obtained results are indicating that the histological analysis of the hepatopancreas proved to be a highly sensitive method for evaluating water quality, in both environmental and laboratory conditions. PMID:25521337

  2. Biological screening of chitosan derivatives using Artemia spp. (brine shrimp test)

    International Nuclear Information System (INIS)

    The present study reported on the screening of six selected chitosan derivatives using the brine shrimp lethality bioassay. In addition, the irradiation effects towards the compounds at 25 kGy were also studied. Chitosan is a natural polysaccharide derived from chitin, extracted from the exoskeletons of crustaceans and insects as well as walls of some bacteria and fungi. Brine shrimp test is employed for the screening of toxicity of chitosan derivatives. Toxicity test was carried out by adding different concentrations of tested samples to approximately 5 to 15 Artemia salina larvae. Biological activity using the brine shrimp bioassay was recorded as LC50 i.e. lethal concentration that kills 50% of the larvae within 24 hours of contact with the samples. Compounds are considered toxic when the LC50 value is lower than 1 mg/ml by brine shrimp bioassay and practically non-toxic when the value is larger. Of the samples tested, none were toxic to the brine shrimp (LC50 > 1 mg/ml). The LC50 values of all chitosan derivatives tested, control and irradiated at 25 kGy were above 1 mg/ml thus all tested samples are considered non-toxic. This study demonstrated that irradiation at 25 kGy showed no significant effects towards the toxicity of the chitosan derivatives. After irradiation, only NO-CMC exhibited marked decrease in LC50 value, reduced by 3-fold from 34.96 mg/ml to 11.07 mg/ml while O-CMC (5.45 mg/ml to 5 mg/ml) showed no clear differences based on rough estimation. This study suggested that brine shrimp bioassay is a simple, reliable and convenient method that could provide useful clues of the relative toxic potential of the sample tested. (Author)

  3. Study on Translating Chinese into Chinese Sign Language

    Institute of Scientific and Technical Information of China (English)

    徐琳; 高文

    2000-01-01

    Sign language is a visual-gestural language mainly used by hearing impaired people to communicate with each other. Gesture and facial expression are important grammar parts of sign language. In this paper, a text-based transfor mation method of Chinese-Chinese sign language machine translation is proposed.Gesture and facial expression models are created. And a practical system is im plemented. The input of the system is Chinese text. The output of the system is "graphics person" who can gesticulate Chinese sign language accompanied by facial expression that corresponds to the Chinese text entered so as to realize automatic translation from Chinese text to Chinese sign language.

  4. Chinese nuclear insurance and Chinese nuclear insurance pool

    International Nuclear Information System (INIS)

    Chinese Nuclear Insurance Started with Daya Bay Nuclear Power Station, PICC issued the insurance policy. Nuclear insurance cooperation between Chinese and international pool's organizations was set up in 1989. In 1996, the Chinese Nuclear Insurance Pool was prepared. The Chinese Nuclear Insurance Pool was approved by The Chinese Insurance Regulatory Committee in May of 1999. The principal aim is to centralize maximum the insurance capacity for nuclear insurance from local individual insurers and to strengthen the reinsurance relations with international insurance pools so as to provide the high quality insurance service for Chinese nuclear industry. The Member Company of Chinese Nuclear Pool and its roles are introduced in this article

  5. Male claspers in clam shrimps (Crustacea, Branchiopoda) in the light of evolution

    DEFF Research Database (Denmark)

    Kaji, Tomonari; Fritsch, Martin; Schwentner, Martin; Olesen, Jørgen; Richter, Stefan

    2014-01-01

    Male "clam shrimps" possess highly modified first (and second) trunk limbs for clasping the carapace of females during copulation. Claspers are present in all three clam shrimp taxa (Laevicaudata, Spinicaudata, and Cyclestherida) but despite striking similarities in their morphology and function......, the matter of their homology is controversial. In this study, we address the question of the homology and evolution of these structures by comparing the developmental transformation of an unspecialized trunk limb into a clasper. In addition, we study the musculature and the nervous system in trunk...

  6. IMPACT OF PARASITISM BY GREGARINES (Nematopsis sp) IN FARMING SHRIMP Litopenaeus vannamei

    OpenAIRE

    Francisco M. Guzmán-Sáenz; Roberto Pérez-Castañeda; Gilberto Gutiérrez-Salazar; Pablo González-Alanís; Mario Hernández-Acosta; Jesús G. Sánchez-Martínez

    2014-01-01

    Infestation of gregarines from genus Nematopsis in Pacific white shrimp Litopenaeus vannamei is commonly associated with a decrease in production and low weight, as well as a possible predisposition to viral infections. The goal of our past research was to evaluate the effect of the Nematopsis sp. gregarine parasitosis on the growth of farm-grown Litopenaeus vannamei shrimp. Four cages were built with PVC pipes and plastic screens with a 6mm light diameter, 1,7m in height, and 1,5m in width ...

  7. Acute Toxicity and Neurotoxicity of Chlorpyrifos in Black Tiger Shrimp, Penaeus monodon

    OpenAIRE

    Tassanee Eamkamon; Sirawut Klinbunga; Kumthorn Thirakhupt; Piamsak Menasveta; Narongsak Puanglarp

    2012-01-01

    Acute toxicity and neurotoxicity of chlorpyrifos were determined in black tiger shrimp, P. monodon. LC50 values after 24 to 96 h of exposure were between 149.55 and 59.16 nmol/L. To determine the neurotoxicity of chlorpyrifos, the inhibition of acetylcholinesterase was monitored in the gill of the shrimps exposed to lethal (0.019, 0.194, and 1.942 µmol/L) and sub-lethal (0.019, 0.194, and 1.942 nmol/L) concentrations of chlorpyrifos. In lethal dose exposure, the AChE activities observed in sh...

  8. Effect of high hydrostatic pressure on the microbiological, biochemical characteristics of white shrimp Litopenaeus vannamei.

    Science.gov (United States)

    Li, Xiu-Xia; Tian, Xin; Li, Jian-Rong

    2016-06-01

    Using thermal processing (TP) treatment (100 ℃, 1-8 min) as a control, the effects of high hydrostatic pressure (HHP, 200-500 MPa, 2.5-20 min) on the microbiological and biochemical characteristics of white shrimp Litopenaeus vannamei were investigated. The results showed that the efficiency of polyphenol oxidase (PPO) inactivation and log reduction of total plate count (TPC) by HHP treatment were all significantly lower than by TP treatment (p treatment was significantly lower than with TP treatment (p treatment turned the appearance of shrimps slightly pink. PMID:26199222

  9. The brine shrimp ( Artemia parthenogenetica) as encapsulation organism for prophylactic chemotherapy of fish and prawn

    Science.gov (United States)

    Cao, Ji-Xiang; Bian, Bo-Zhong; Li, Ming-Ren

    1996-06-01

    Brine shrimp ( Artemia parthenogenetica) which had ingested three water-insoluble antibacterial drugs i.e. sulfadiazine(SD), oxytetracycline (OTC) and erythromycin estolate (ERY-Es) were fed to Tilapia and Mysis III of Penaeus orientalis K. The drug contents in the predators were then determined. After administration of drugs to Tilapia and Mysis III, through the bio-encapsulation of the brine shrimp, efficacious therapeutical concentration of OTC and ERY-Es (but not SD) in the predators could be reached and maintained for more than 8 hours.

  10. Phylogeography and local endemism of the native Mediterranean brine shrimp Artemia salina (Branchiopoda: Anostraca)

    DEFF Research Database (Denmark)

    Muñoz, Joaquin; Gómez, Africa; Green, Andy J.;

    2008-01-01

    There has been a recent appreciation of the ecological impacts of zooplanktonic species invasions. The North American brine shrimp Artemia franciscana is one such alien invader in hyper-saline water ecosystems at a global scale. It has been shown to outcompete native Artemia species, leading to...... their local extinction. We used partial sequences of the mitochondrial Cytochrome c Oxidase Subunit 1 (COI or cox1) gene to investigate the genetic diversity and phylogeography of A. salina, an extreme halophilic sexual brine shrimp, over its known distribution range (Mediterranean Basin and South...

  11. Assessing Ecological Impacts of Shrimp and Sewage Effluent: Biological Indicators with Standard Water Quality Analyses

    Science.gov (United States)

    Jones, A. B.; O'Donohue, M. J.; Udy, J.; Dennison, W. C.

    2001-01-01

    Despite evidence linking shrimp farming to several cases of environmental degradation, there remains a lack of ecologically meaningful information about the impacts of effluent on receiving waters. The aim of this study was to determine the biological impact of shrimp farm effluent, and to compare and distinguish its impacts from treated sewage effluent. Analyses included standard water quality/sediment parameters, as well as biological indicators including tissue nitrogen (N) content, stable isotope ratio of nitrogen (δ 15N), and amino acid composition of inhabitant seagrasses, mangroves and macroalgae. The study area consisted of two tidal creeks, one receiving effluent from a sewage treatment plant and the other from an intensive shrimp farm. The creeks discharged into the western side of Moreton Bay, a sub-tropical coastal embayment on the east coast of Australia. Characterization of water quality revealed significant differences between the creeks, and with unimpacted eastern Moreton Bay. The sewage creek had higher concentrations of dissolved nutrients (predominantly NO-3/NO-2 and PO3-4, compared to NH+4 in the shrimp creek). In contrast, the shrimp creek was more turbid and had higher phytoplankton productivity. Beyond 750 m from the creek mouths, water quality parameters were indistinguishable from eastern Moreton Bay values. Biological indicators detected significant impacts up to 4 km beyond the creek mouths (reference site). Elevated plant δ 15N values ranged from 10·4-19·6‰ at the site of sewage discharge to 2·9-4·5‰ at the reference site. The free amino acid concentration and composition of seagrass and macroalgae was used to distinguish between the uptake of sewage and shrimp derived N. Proline (seagrass) and serine (macroalgae) were high in sewage impacted plants and glutamine (seagrass) and alanine (macroalgae) were high in plants impacted by shrimp effluent. The δ 15N isotopic signatures and free amino acid composition of inhabitant

  12. Signal Analysis for Assessment and Prediction of the Artificial Habitat in Shrimp Aquaculture

    Science.gov (United States)

    Hernández, José Juan Carbajal; Sanchez Fernandez, Luis Pastor; Oropeza Rodríguez, José Luis; Felipe Riverón, Edgardo Manuel

    This paper presents a novel work for prediction of artificial habitat in shrimp aquaculture based on environmental signal analysis. The physical-chemical variables that are involved into the system are studied for modeling and predicting environmental patterns.The prediction model is built using AR models that reconstruct a partial section of a particular measured signal. The physical-chemical variables are classified based on the negative ecological impact using a new statistical model that calculates the frequency and the deviation of the measurements. A fuzzy inference system processes the level classifications using aquaculture rules that define all the cases calculating the condition of the shrimp habitat.

  13. Environmental Impact Assessment of Shrimp Culture Practice in Southwest Coastal Region of Bangladesh

    Science.gov (United States)

    Islam, M. M.; Rouf, M. A.; Hambrey, J.

    2008-12-01

    The rapid unplanned expansion of coastal aquaculture in Bangladesh poses risks in degrading environment. The unsustainable aquaculture practices are suspected to impart significant nutrient load to the ecosystem. Nevertheless, shrimp production is very less and susceptible to sever mortality. For this, the study aimed to understand the management practices and nutrient flux from some represented shrimp ponds in southwest coastal region of Bangladesh. A comprehensive study from 2002 to 2004 was done in an aquatic system comprising a river (Semi diurnal tidal system), a canal and 10 shrimp ponds along with wider area survey to verify the issues under a DFID-funded research project involving Nautilus Consultants Ltd. (UK). In the study area (Dumuria under the district Khulna) shrimp culture practices were mostly improved extensive. Shrimp were being produced without proper pond preparation, fry nursing, stocking or feed management due to lack of technical somehow and the risks involved with higher levels of investment. Consequently production rates were very low, averaging only 191 Kg/ha. Very high mortality of shrimp was reported in some of the farms using shallow ponds immediately after heavy rainfall. This may due to the sudden fluctuation of water pH and/or water temperature and salinity. The water quality in shrimp ponds during grow out period was in acceptable range except lower trend of DO and ammonia nitrogen. Year round water quality observations indicated that there were no major differences among the river, canal and pond water that might be due to the high water flushing rate (468%). The nutrient dynamics estimation indicates that 48.7 kg/ha/cycle of nitrogen and 28.96 kg/ha/cycle of phosphorus were added to the pond as an input mostly from fertilizer and feed. Among the inputs, only 33.4% of nitrogen and 6% of phosphorus were removed as harvested form. A large portion, 39.1% N and 92% P were remained in the sediments and unaccounted for. Average intake of

  14. Low-input Modified Extensive Shrimp Culture System for Penaeus monodon Restrian Vibriosis

    Digital Repository Service at National Institute of Oceanography (India)

    Fernandes, S.O.; Sreepada, R.A.; Kulkarni, S.S.; Karekar. S.V.; Shirodkar, R.R.; Vogelsang, C.; LokaBharathi, P.A.

    .10 × 103 CFU g-1. Counts of LB were found to be below detectable levels in hepatopancreas between 105 to 135-doc. 1.3 Correlation analysis None of the measured environmental variables (temperature, salinity, pH, DO) showed any significant correlation... = 65, pond sediment = 29 and shrimp hepatopancreas = 20 International Journal of Marine Science 2013, Vol.3, No.40, 319-332 http://ijms.sophiapublisher.com 324 1.5 Average weight, feed conversion ratio and survival Cultured shrimps attained...

  15. Research in Ancient Chinese Language

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    JIANG Ji-cheng, A Brief Study of Arabian-Chinese Diaphone in Huihui Prescription Abstract: Based on meterials of Arabian- Chinese diaphone in Huihui Prescription, this paper studies all Chinese phonetic initials and finals in Yuan dynasty, including 21 initials and 34 finals. Key Words: Huihui Prescription, Arabian- Chinese diaphone, transferred sound, International Phonetic Alphabet

  16. Geographical distribution of pelagic decapod shrimp in the Atlantic Ocean.

    Science.gov (United States)

    Judkins, David C

    2014-01-01

    Ninety-one species of pelagic decapod shrimp were identified in 938 midwater-trawl collections taken between 1963 and 1974 from the North and South Atlantic. Distributional maps are provided for the most frequently occurring species. Nighttime abundance of most species was greatest within the upper 200 m. Degree of geographical overlap was estimated using the geometric mean of the proportion of joint occurrences with a value ≥ 0.5 deemed significant. Geographical distributions tended to be unique, and only 31 species had values ≥ 0.5 with one or more other species. Species within genera and within phylogenetic subgroups of Sergia were generally parapatric or partially overlapping in distribution. Five geographical groupings of co-occurring species across genera were identified: Subpolar-Temperate, Southern Hemisphere, Central, Tropical, Eastern Tropical and Western Tropical. The two species of the Southern Hemisphere group are circumpolar at temperate latitudes. The 12 species of the Central group occurred throughout the subtropical and tropical North and South Atlantic. The eight species of the Tropical group occurred broadly across the equatorial Atlantic and Caribbean with ranges usually extending into the Gulf of Mexico and northward in the Gulf Stream. The two species of the Western Tropical group occurred most often in the western tropics, but there were scattered occurrences at subtropical latitudes. The four species of the Eastern Tropical group were endemic to the Mauritanian Upwelling and the Angola-Benguela Frontal zones off western Africa. Two of the three species in the Subpolar-Temperate group had bipolar distributions, and all three occurred in the Mediterranean and in the Mauritanian Upwelling zone. Most Central, Tropical and Western Tropical species were present in the in the Gulf of Mexico. The 10 species from the Mediterranean were a mixture of Subpolar-Temperate, Central and benthopelagic species. Patterns of distribution in Atlantic pelagic

  17. The Brine Shrimp Artemia: Adapted to Critical Life Conditions

    Science.gov (United States)

    Gajardo, Gonzalo M.; Beardmore, John A.

    2012-01-01

    The brine shrimp Artemia is a micro-crustacean, well adapted to the harsh conditions that severely hypersaline environments impose on survival and reproduction. Adaptation to these conditions has taken place at different functional levels or domains, from the individual (molecular-cellular-physiological) to the population level. Such conditions are experienced by very few equivalent macro-planktonic organisms; thus, Artemia can be considered a model animal extremophile offering a unique suite of adaptations that are the focus of this review. The most obvious is a highly efficient osmoregulation system to withstand up to 10 times the salt concentration of ordinary seawater. Under extremely critical environmental conditions, for example when seasonal lakes dry-out, Artemia takes refuge by producing a highly resistant encysted gastrula embryo (cyst) capable of severe dehydration enabling an escape from population extinction. Cysts can be viewed as gene banks that store a genetic memory of historical population conditions. Their occurrence is due to the evolved ability of females to “perceive” forthcoming unstable environmental conditions expressed by their ability to switch reproductive mode, producing either cysts (oviparity) when environmental conditions become deleterious or free-swimming nauplii (ovoviviparity) that are able to maintain the population under suitable conditions. At the population level the trend is for conspecific populations to be fragmented into locally adapted populations, whereas species are restricted to salty lakes in particular regions (regional endemism). The Artemia model depicts adaptation as a complex response to critical life conditions, integrating and refining past and present experiences at all levels of organization. Although we consider an invertebrate restricted to a unique environment, the processes to be discussed are of general biological interest. Finally, we highlight the benefits of understanding the stress response of

  18. The brine shrimp Artemia: adapted to critical life conditions

    Directory of Open Access Journals (Sweden)

    Gonzalo M Gajardo

    2012-06-01

    Full Text Available The brine shrimp Artemia is a micro-crustacean, well adapted to the harsh conditions that severely hypersaline environments impose on survival and reproduction. Adaptation to these conditions has taken place at different functional levels or domains, from the individual (molecular-cellular-physiological to the population level. Such conditions are experienced by very few equivalent macro-planktonic organisms; thus, Artemia can be considered a model animal extremophile offering a unique suite of adaptations that are the focus of this review. The most obvious is a highly efficient osmoregulation system to withstand up to 10 times the salt concentration of ordinary seawater. Under extremely critical environmental conditions, for example when seasonal lakes dry out, Artemia takes refuge by producing a highly resistant encysted gastrula embryo (cyst capable of severe dehydration enabling an escape from population extinction. Cysts can be viewed as gene banks that store a genetic memory of historical population conditions. Their occurrence is due to the evolved ability of females to perceive forthcoming unstable environmental conditions expressed by their ability to switch reproductive mode, producing either cysts (oviparity when environmental conditions become deleterious or free-swimming nauplii (ovoviviparity that are able to maintain the population under suitable conditions.At the population level the trend is for conspecific populations to be fragmented into locally adapted populations, whereas species are restricted to salty lakes in particular regions (regional endemism. The Artemia model depicts adaptation as a complex response to critical life conditions, integrating and refining past and present experiences at all levels of organization. Although we consider an invertebrate restricted to a unique environment, the processes to be discussed are of general biological interest. Finally, we highlight the benefits of understanding the stress

  19. Northern Shrimp (Pandalus borealis) Recruitment in West Greenland Waters. Part I. Distribution of Pandalus Shrimp Larvae in Relation to Hydrography and Plankton

    DEFF Research Database (Denmark)

    Pedersen, S. A.; Storm, L. M.; Simonsen, C. S.

    2002-01-01

    Plankton samples and oceanographic data were obtained during transect studies across fishing banks over the West Greenland shelf areas in June 1999, May, and July 2000. The hypothesis that larval shrimp are linked to the behaviour of hydrographic fronts was tested by determining whether larval...... shrimp abundance was linked to plankton distributions, species composition and primary productivity. We found six pelagic developmental stages (ZI–ZVI) in two species of Pandalus larvae. P. borealis was the most abundant species in all stages from ZII to ZVI. The smaller P. montagui larvae were slightly...... more advanced in development than P. borealis, suggesting later hatching or longer development time for P. borealis larvae. In May, high concentrations of newly hatched ZI larvae were caught near the coast and at fjord stations. In June and July, larvae in development stage ZIV dominated the catches...

  20. Determination of Profiles of Salmonella and Pathogenic Vibrio SPP. in Black Tiger Shrimp for Export by Introduction of Quality Assured Microbiological Assays

    International Nuclear Information System (INIS)

    Studies were conducted on contamination by Salmonella and pathogenic Vibrio spp. in samples of aquaculture black tiger shrimp, the water supply (canal water which is supplied as raw pond water before treatment), pond water, feed materials, and fresh and frozen shrimp. Salmonella was detected in samples of the water supply, pond water, feed materials, fresh shrimp at farm, fresh shrimp from wholesale market and frozen shrimp destined for export at levels of 13.95%, 1.53%, 1.14%, 3.17%, 30.4% and 0.21% respectively. V. cholerae non 01 was found in one sample of water from a culture pond of 131 tested (0.8%). V. parahaemolyticus was found in samples of canal water, pond water, fresh black tiger shrimp collected at farms, fresh black tiger shrimp collected at wholesale shrimp markets and frozen black tiger shrimp destined for export at levels of 2.3%, 5.3%, 14.3%, 48 % and 0.2% respectively. The strains identified as V. parahaemolyticus were examined for the presence or absence of the TDH and TRH. The incidence of TDH (KP+) was 2.67% (seven of 262 strains) and of TRH (urease reaction) was 1.15% (three of 262 strains). Salmonella and V. parahaemolyticus were found in a high percentage in fresh black tiger shrimp collected from wholesale shrimp markets. These shrimp are used as raw material for domestic consumption and for processing for exported shrimp products. Therefore GMP and/or Hazard Analysis Critical Control Point (HACCP) system for shrimp distributors/producers should be applied. (author)

  1. Success factors of Chinese restaurants

    OpenAIRE

    Aakala, Liwen Heli

    2009-01-01

    The objectives of the thesis fall into three aspects: 1) understanding Chinese entrepreneurship through some major aspects; such as, the characteristics of Chinese entrepreneur and successful skills needed; 2) scanning the Chinese culture that is associated with their entrepreneurial success in restaurant business as well as understanding the Finnish culture that affects Chinese restaurants’ presence in Finland; 3) acquainting with the competitive strategies that those Chinese restaurants emp...

  2. Let's make the brown shrimp green ! Evaluation of discarding practices in the North Sea Brown shrimp (Crangon crangon L.) fishery

    OpenAIRE

    H. POLET

    2005-01-01

    The Brown Shrimp (Crangon crangon L.) fishery in the North Sea, the focus of this study, is carried out with small meshed nets in vulnerable areas like coastal zones and estuaries. The discarding practices associated with it have been regarded as a problem for many years. The discussion, however, was difficult since no sufficiently reliable discard data were available. The need for data on this issue and a solution for the discard problem was the starting point of the study. The main objectiv...

  3. The Contrast of Chinese and English in the Translation of Chinese Poetry

    OpenAIRE

    Ning Li

    2009-01-01

    Chinese poetry is the soul of Chinese literature and Chinese culture. A good translation of a Chinese verse can promote the prevalence of Chinese culture. In the translation of Chinese poetry, translators should not only keep the characteristics of Chinese poems, but also embody the English characteristics. This article analyzed some versions of translation and proposed factors affecting the translation of Chinese poetry.

  4. Effect of gamma-irradiation on frozen shrimps for inactivation of microorganisms and shelf-life extension under defrosted

    International Nuclear Information System (INIS)

    The effect of gamma-irradiation on inactivation of microorganisms in frozen shrimps was investigated for improving the hygienic quality and shelf-life after defrosted. Count of total aerobic bacteria in three different kinds of frozen shrimps were decreased below 104 per gram at 4 kGy of irradiation, and showed the similar pattern of inactivation curves under the same frozen condition . Coliforms were eliminated below 4 kGy of irradiation. Radiation-resistance of aerobic bacteria in frozen condition was larger than defrosted condition, and the necessary dose for inactivation of aerobic bacteria below the count of 104 per gram was required 3 kGy at defrosted condition, and 4 kGy at frozen condition. However, freezing could remarkably reduce the production of radiation induced off-odor from shrimps. Trimethylamine content in frozen shrimps did not get effect up to 50 kGy irradiation. Vibrio parahaemolyticus was isolated from 1 to 3 numbers in 100g of frozen shrimps, and necessary dose for elimination was decided as 1.5 to 2.0 kGy in frozen shrimps. Shelf-life of irradiated shrimps at 3 and 5 kGy was found to be 6 days at the storage condition of 5degC, whereas the unirradiated samples were putrefied below 3 days of storage. (author)

  5. Cadmium concentrations in shrimp (Penaeus semisulcatus and Penaeus monodon) caught from the coastal areas in Southern Iran.

    Science.gov (United States)

    Rahimi, E; Zaker, Sh; Farfani, M H Dehghani; Araghi, M Roghani; Vafaei, M; Goudarzi, M A

    2013-04-01

    This study was undertaken to determine the concentration of cadmium in two shrimp species, namely, Penaeus semisulcatus and Penaeus monodon caught from the coastal areas in southern Iran. Cadmium concentration was determined by graphite furnace atomic absorption spectrophotometry in 91 shrimp samples after nitric acid/perchloric acid digestion. Accuracy of the analysis was checked by various methods including the use of reference material. The mean ± SD of cadmium concentrations in shrimp samples were 0.128 ± 0.144 (μg/g). The cadmium concentrations ranged from 0.010 to 0.96 μg/g of the muscle tissues of shrimp. Higher cadmium concentration in shrimp samples was found in summer (significant p cadmium in shrimp is lower than the maximum allowed levels according to International standards, although the concentration of cadmium in only one sample was more than the amount recommended by Food and Agriculture Organization. Therefore, no risk to the consumer arises from the cadmium contents of the shrimp caught in these areas. PMID:22301817

  6. Metal and metalloid bioaccumulation in the Pacific blue shrimp Litopenaeus stylirostris (Stimpson) from New Caledonia: laboratory and field studies.

    Science.gov (United States)

    Metian, Marc; Hédouin, Laetitia; Eltayeb, Mohamed M; Lacoue-Labarthe, Thomas; Teyssié, Jean-Louis; Mugnier, Chantal; Bustamante, Paco; Warnau, Michel

    2010-01-01

    The present work aimed at better understanding metal and metalloid bioaccumulation in the edible Pacific blue shrimp Litopenaeus stylirostris, using both laboratory and field approaches. In the laboratory, the bioaccumulation kinetics of Ag, Cd, Co, Cr, and Zn have been investigated in shrimp exposed via seawater and food, using the corresponding gamma-emitting radiotracers ((110 m)Ag, (109)Cd, (57)Co, (51)Cr, and (65)Zn) and highly sensitive nuclear detection techniques. Results showed that hepatopancreas and intestine concentrated the metals to the highest extent among the blue shrimp organs and tissues. Moulting was found to play a non negligible detoxification role for Co, Cr and, to a lesser extent, Zn. Metal retention by L. stylirostris widely varied (from a few days to several months), according to the element and exposure pathway considered (a given metal was usually less strongly retained when ingested with food than when it was taken up from the dissolved phase). In the field study, Ag, As, Cd, Co, Cr, Cu, Mn, Ni, and Zn were analysed in shrimp collected from a New Caledonian aquaculture pond. Metal concentrations in the shrimp muscles were generally relatively low and results confirmed the role played by the digestive organs and tissues in the bioaccumulation/storage/detoxification of metals in the Pacific blue shrimp. Preliminary risk considerations indicate that consumption of the shrimp farmed in New Caledonia is not of particular concern for human health. PMID:20637480

  7. Effect of low-dose gamma irradiation on the shelf-life and quality of frozen shrimp (Penaeus merquiensis)

    International Nuclear Information System (INIS)

    The effects of low-dose gamma irradiation on the shelf-life and quality of frozen shrimp (Penaeus merquiensis) has been studied. Fresh peeled shrimp and shell on headless shrimp were frozen and irradiated at doses of 2 and 4 kGy and held at -18 degC for seven months. Changes in total aerobic count, pH, trimethylamine nitrogen, total volatile basic nitrogen, rancidity and sensory of irradiated and non-irradiated frozen peeled and shell on headless shrimp were determined. Both frozen peeled and shell on headless shrimp, non-irradiated and irradiated at 2 and 4 kGy, were found to be within the acceptable range on a nine point hedonic scale even after seven months of frozen storage. However, storage period should not be more than four months, from a viewpoint of quality retention. Irradiation of frozen shrimp at doses of 2 and 4 kGy reduced microbial levels by 2-3 log cycles. The irradiated product was found to be free of faecal coliform and Escherichia coli. The chemical, physical, and bacteriological attributes used as objective indices did not serve as a useful index of quality. Their values were low and variable during storage to correlate them with sensory quality. It is proposed that dose of 2 kGy is adequate for a radurized frozen shrimp process

  8. High prevalence of Enterocytozoon hepatopenaei in shrimps Penaeus monodon and Litopenaeus vannamei sampled from slow growth ponds in India.

    Science.gov (United States)

    Biju, Narayanan; Sathiyaraj, Ganesan; Raj, Mithun; Shanmugam, Venu; Baskaran, Babu; Govindan, Umamaheswari; Kumaresan, Gayathri; Kasthuriraju, Karthick Kannan; Chellamma, Thampi Sam Raj Yohannan

    2016-08-01

    Hepatopancreatic microsporidiosis in cultivated Litopenaeus vannamei and Penaeus monodon is caused by the newly emerged pathogen Enterocytozoon hepatopenaei (EHP). It has been detected in shrimp cultured in China, Vietnam and Thailand and is suspected to have occurred in Malaysia and Indonesia and to be associated with severely retarded growth. Due to retarded shrimp growth being reported at farms in the major grow-out states of Tamilnadu, Andhra Pradesh and Odisha in India, shrimp were sampled from a total of 235 affected ponds between March 2014 and April 2015 to identify the presence of EHP. PCR and histology detected a high prevalence of EHP in both P. monodon and L. vannamei, and infection was confirmed by in situ hybridization using an EHP-specific DNA probe. Histology revealed basophilic inclusions in hepatopancreas tubule epithelial cells in which EHP was observed at various developmental stages ranging from plasmodia to mature spores. The sequence of a region of the small subunit rDNA gene amplified by PCR was found to be identical to EHP sequences deposited in GenBank. Bioassays confirmed that EHP infection could be transmitted orally to healthy shrimp. Histology also identified bacterial co-infections in EHP-infected shrimp sampled from slow-growth ponds with low-level mortality. The data confirm that hepatopancreatic microsporidiosis caused by EHP is prevalent in shrimp being cultivated in India. EHP infection control measures thus need to be implemented urgently to limit impacts of slowed shrimp growth. PMID:27503918

  9. Economic aspects of the introduction of radiation preservation of brown shrimp in the Federal Republic of Germany

    International Nuclear Information System (INIS)

    Radurization of brown shrimp and shrimp meat to improve the shelflife and the hygienic quality is technically and economically feasible. Prospects of the process for the shrimp fisheries in the Federal Republic of Germany are good. Costs for treatment with a dose of 100 krad are only 0.10 DM per kg under favorable conditions, and about 2.00 DM per kg under the most unfavorable conditions. In view of the benefits to consumer and producer, and in relation to retail prices which sometimes exceed 25.00 DM per kg, such costs appear reasonable. Economic conditions of shrimp fisheries favour the first installation at a central location in Schleswig-Holstein where about one half of the total German landings could be irradiated. It is suggested to test the market and consumer reactions with experimental lots of shrimp irradiated by use of a mobile irradiator at one of the shrimp harbours. Such a commercial-scale experiment should render enough data to determine whether an investment in a permanent irradiator for brown shrimp will be worthwhile. (author)

  10. Bioconcentration of the anionic surfactant linear alkylbenzene sulfonate (LAS) in the marine shrimp Palaemonetes varians: A radiotracer study

    International Nuclear Information System (INIS)

    Highlights: • The marine shrimp Palaemonetes varians concentrates waterborne linear alkylbenzene sulfonate (LAS). • Size/weight plays a role in the LAS concentration in shrimp. • The LAS previously concentrated in the shrimp are rapidly depurated and weakly retained. • Shrimp’s target compartments for concentration of waterborne LAS are mainly located in the cephalothorax. - Abstract: Uptake and depuration kinetics of dissolved [14C]C12-6-linear alkylbenzene sulfonate (LAS) were determined in the shrimp Palaemonetes varians using environmentally relevant exposure concentration. The shrimp concentrated LAS from seawater with a mean BCF value of 120 L kg−1 after a 7-day exposure. Uptake biokinetics were best described by a saturation model, with an estimated BCFss, of 159 ± 34 L kg−1, reached after 11.5 days. Shrimp weight influenced significantly BCF value with smaller individuals presenting higher affinity to LAS. To the light of a whole body autoradiography, major accumulation of LAS occurred in the cephalothorax circulatory system (gills, heart, hepatopancreas) and ocular peduncle, but not in the flesh, limiting potential transfer to human consumers. LAS depuration rate constant value of the shrimp was 1.18 ± 0.08 d−1 leading to less than 1% of remaining LAS in its tissues after 8 days of depuration

  11. Shrimp predation on 0-group plaice: contrasts between field data and predictions of an individual-based model

    Science.gov (United States)

    Burrows, Michael T.; Gontarek, Steven J.; Nash, Richard D. M.; Gibson, Robin N.

    2001-06-01

    An individual-based model has been used to predict the effects of size-dependent predation by shrimp Crangon crangon on populations of newly settled 0-group plaice Pleuronectes platessa. The model predicted changes in population size and thus mortality rate of plaice during settlement and the following period until the plaice reached 30 mm in length and were invulnerable to further shrimp predation. Population parameters derived from seven years' catch data for 0-group plaice and shrimp from the west coast of Scotland were supplied to the model for direct comparison of predicted and observed mortality rates for each of the seven years. The contrast of predicted and observed mortality was used to test the hypothesis that predation by shrimp is a major factor regulating populations of juvenile plaice. There was no evidence for control of plaice populations by shrimp predation at the study site, since a negative relationship between plaice mortality and shrimp density was observed. Alternative explanations for the divergence of prediction and observations included (i) a general failure of the model to adequately describe predator and prey populations, (ii) limited effects of consumption of plaice by shrimp, and (iii) responses of both species to a third factor such as temperature producing an apparent association of survival and recruitment in the respective species.

  12. Cultural Characteristics of Chinese Cuisine:From Contrastive Studies of English and Chinese

    Institute of Scientific and Technical Information of China (English)

    乞聪妮

    2014-01-01

    Chinese cuisine plays an important role in Chinese culture. The paper illustrates the features of Chinese cuisine in Chi-nese dish naming from different perspectives, and analyze them from contrastive studies of English and Chinese.

  13. Chinese Female Creativity

    Institute of Scientific and Technical Information of China (English)

    VALERIE; SARTOR

    2011-01-01

    "Many foreigners mistakenly believe that Chinese women are creatively oppressed,that they have been oppressed for centuries," Teacher Yang said,glancing at me wryly."That’s correct," I replied, lifting my eyebrows.

  14. Traditional Chinese Biotechnology

    Science.gov (United States)

    Xu, Yan; Wang, Dong; Fan, Wen Lai; Mu, Xiao Qing; Chen, Jian

    The earliest industrial biotechnology originated in ancient China and developed into a vibrant industry in traditional Chinese liquor, rice wine, soy sauce, and vinegar. It is now a significant component of the Chinese economy valued annually at about 150 billion RMB. Although the production methods had existed and remained basically unchanged for centuries, modern developments in biotechnology and related fields in the last decades have greatly impacted on these industries and led to numerous technological innovations. In this chapter, the main biochemical processes and related technological innovations in traditional Chinese biotechnology are illustrated with recent advances in functional microbiology, microbial ecology, solid-state fermentation, enzymology, chemistry of impact flavor compounds, and improvements made to relevant traditional industrial facilities. Recent biotechnological advances in making Chinese liquor, rice wine, soy sauce, and vinegar are reviewed.

  15. Chinese remainder codes

    Institute of Scientific and Technical Information of China (English)

    ZHANG Aili; LIU Xiufeng

    2006-01-01

    Chinese remainder codes are constructed by applying weak block designs and the Chinese remainder theorem of ring theory.The new type of linear codes take the congruence class in the congruence class ring R/I1 ∩ I2 ∩…∩ In for the information bit,embed R/Ji into R/I1 ∩ I2 ∩…∩ In,and assign the cosets of R/Ji as the subring of R/I1 ∩ I2 ∩…∩ In and the cosets of R/Ji in R/I1 ∩ I2 ∩…∩ In as check lines.Many code classes exist in the Chinese remainder codes that have high code rates.Chinese remainder codes are the essential generalization of Sun Zi codes.

  16. Chinese Remainder Codes

    Institute of Scientific and Technical Information of China (English)

    张爱丽; 刘秀峰; 靳蕃

    2004-01-01

    Chinese Remainder Codes are constructed by applying weak block designs and Chinese Remainder Theorem of ring theory. The new type of linear codes take the congruence class in the congruence class ring R/I1∩I2∩…∩In for the information bit, embed R/Ji into R/I1∩I2∩…∩In, and asssign the cosets of R/Ji as the subring of R/I1∩I2∩…∩In and the cosets of R/Ji in R/I1∩I2∩…∩In as check lines. There exist many code classes in Chinese Remainder Codes, which have high code rates. Chinese Remainder Codes are the essential generalization of Sun Zi Codes.

  17. Traditional Chinese biotechnology.

    Science.gov (United States)

    Xu, Yan; Wang, Dong; Fan, Wen Lai; Mu, Xiao Qing; Chen, Jian

    2010-01-01

    The earliest industrial biotechnology originated in ancient China and developed into a vibrant industry in traditional Chinese liquor, rice wine, soy sauce, and vinegar. It is now a significant component of the Chinese economy valued annually at about 150 billion RMB. Although the production methods had existed and remained basically unchanged for centuries, modern developments in biotechnology and related fields in the last decades have greatly impacted on these industries and led to numerous technological innovations. In this chapter, the main biochemical processes and related technological innovations in traditional Chinese biotechnology are illustrated with recent advances in functional microbiology, microbial ecology, solid-state fermentation, enzymology, chemistry of impact flavor compounds, and improvements made to relevant traditional industrial facilities. Recent biotechnological advances in making Chinese liquor, rice wine, soy sauce, and vinegar are reviewed. PMID:19888561

  18. Assessment of cyst production potential of a natural population of brine shrimp Artemia

    Digital Repository Service at National Institute of Oceanography (India)

    Royan, J.P.; Sumitra-Vijayaraghavan; Krishnakumari, L.

    Studies on a natural population of Artemia in the salterns of Jamnagar indicated that the population is parthenogenetic. These shrimps reach a maximum size of 9 mm. Number of cysts per brood varies from 10-32. Adults form about 68% of the total...

  19. Mercury content and their risk assessment in farmed shrimp Litopenaeus vannamei from NW Mexico.

    Science.gov (United States)

    Delgado-Alvarez, C G; Ruelas-Inzunza, J; Osuna-López, J I; Voltolina, D; Frías-Espericueta, M G

    2015-01-01

    The main objective of this study was to evaluate the total mercury content in hepatopancreas and edible muscle of the whiteleg shrimp Litopenaeus vannamei cultured along the NW coast of Mexico, and to evaluate the potential human health risk due to their consumption. Samples were obtained between May and June 2010 in 26 shrimp farms from the three most important shrimp-producing states of NW Mexico, and total Hg was analyzed after reduction with SnCl2 in a mercury analyzer. The ranges of Hg concentrations of the hepatopancreas were 0.101±0.03-0.184±0.13 μg g(-1) in Sonora, 0.077±0.055-0.813±0.363 μg g(-1) in Sinaloa and 0.139±0.037-0.791±0.33 μg g(-1) in Nayarit. In the muscle, values were from 0.078±0.02 to 0.539±0.09 μg g(-1) in Sonora, 0.154±0.03-0.861±0.423 μg g(-1) in Sinaloa and 0.121±0.041-1.48±0.44 μg g(-1) in Nayarit. Considering the concentrations of Hg in the muscle and the national consumption rate, shrimp farmed in NW Mexico does not represent a risk for human health (HQ<1). PMID:25303662

  20. Genetic and morphological differentiation in the Sakura shrimp (Sergia lucens) between Japanese and Taiwanese populations

    NARCIS (Netherlands)

    Imai, H.; Hanamura, Y.; Cheng, J.-H.

    2013-01-01

    The Sakura shrimp Sergia lucens is a remarkable meso-pelagic crustacean species, which is harvested for human consumption in restricted geographical areas of Taiwan and Japan in the northwestern Pacific. Nucleotide sequence analysis of the mitochondrial DNA control region was conducted to investigat

  1. Acerola Fruit as a Possible Antimelanotic Agent in White Shrimp (Litopenaeus vannamei

    Directory of Open Access Journals (Sweden)

    Alex Augusto Gonçalves

    2015-01-01

    Full Text Available This study aimed to testify acerola’s inhibitory activity on melanosis in farmed white shrimp (L. vannamei. Shrimp samples underwent immersion in different treatments (control group (C, metabisulphite solution group (M, and acerola solution group (A. Quality Index Method (QIM was used to measure the shrimp shelf life, during 21 days, where microbiological, chemical, and sensory analyses were performed at each 3 days. Groups C and A exceeded the limit of mesophilic bacterial count within 15 days of storage, and for M the period was 18 days. As for the psychrophilic bacteria, C and A both crossed the limit on day 12 and M on the 15th day. Group M had the highest TVBN and pH means, followed by A and lastly C. Yet, TMA analysis showed highest values in group C, second A, and finally M. Sensory analysis determined the shelf life of groups C, M, and A as 10.5, 14.6, and 12.3 days, respectively. There were not many significant differences between the groups in all analyses; hence, dipping shrimp in acerola solutions would not suffice to inhibit melanosis formation. Further studies are needed to fully determine acerola’s antimelanosic potential.

  2. Assessment of nitrogen and sulphur cycle bacteria and shrimp production in ponds treated with biological products

    Institute of Scientific and Technical Information of China (English)

    Thangapalam Jawahar Abraham; Shubhadeep Ghosh; Debasis Sasmal

    2015-01-01

    Objective:To study the influence of biological products on the levels of nitrogen and sulphur cycle bacteria in shrimp culture systems of West Bengal, India. Methods: The pond water and sediment samples were analyzed for physico-chemical parameters as per standard methods. The bacteria involved in ammonification, nitrification, denitrification, sulphate reduction and sulphur oxidation were enumerated by most probable number technique. Results:The semi-intensive and modified extensive shrimp farms used a variety of biological products during various stages of production. No biological products were used in traditional farms. The water and sediment samples of modified extensive system recorded significantly higher mean heterotrophic bacterial counts. The counts of ammonia, nitrite and sulphur oxidizers, and nitrate and sulphate reducers varied among the systems. The cycling of nitrogen and sulphur appeared to be affected with the intensification of culture practices. Conclusions:The application of biological products in certain systems helped to maintain the bacteria involved in nitrogen and sulphur cycles and safe levels of ammonia, nitrite and nitrate. An assessment of these metabolically active bacteria in shrimp culture ponds and the application of right kind microbial products would help ameliorate the organic pollution in shrimp aquaculture.

  3. DISTINCTIVE LOCALIZATION OF GROUP 3 LATE EMBRYOGENESIS ABUNDANT SYNTHESIZING CELLS DURING BRINE SHRIMP DEVELOPMENT.

    Science.gov (United States)

    Kim, Bo Yong; Song, Hwa Young; Kim, Mi Young; Lee, Bong Hee; Kim, Kyung Joo; Jo, Kyung Jin; Kim, Suhng Wook; Lee, Seung Gwan; Lee, Boo Hyung

    2015-07-01

    Despite numerous studies on late embryogenesis abundant (LEA) proteins, their functions, roles, and localizations during developmental stages in arthropods remain unknown. LEA proteins protect crucial proteins against osmotic stress during the development and growth of various organisms. Thus, in this study, fluorescence in situ hybridization was used to determine the crucial regions protected against osmotic stress as well as the distinctive localization of group 3 (G3) LEA(+) cells during brine shrimp development. Several cell types were found to synthesize G3 LEA RNA, including neurons, muscular cells, APH-1(+) cells, and renal cells. The G3 LEA(+) neuronal cell bodies outside of the mushroom body projected their axonal bundles to the central body, but those inside the mushroom body projected their axonal bundles toward the deutocerebrum without innervating the central body. The cell bodies inside the mushroom body received axons of the G3 LEA(+) sensory cells at the medial ventral cup of the nauplius eye. Several glands were found to synthesize G3 LEA RNA during the nauplius stages of brine shrimp, including the sinus, antennal I and II, salt, and three ectodermal glands. This study provides the first demonstration of the formation of G3 LEA(+) sinus glands at the emergence stages of brine shrimp. These results suggest that G3 LEA protein is synthesized in several cell types. In particular, specific glands play crucial roles during the emergence and nauplius stages of brine shrimp. PMID:25781424

  4. Seasonal and laboratory variations in the health of grass shrimp Palaemonetes pugio: dodecyl sodium sulfate bioassay

    Energy Technology Data Exchange (ETDEWEB)

    Tatem, H.E. (Waterways Experiment Station, Vicksburg, MS); Anderson, J.W.; Neff, J.M.

    1976-09-01

    The reported study involved numerous static toxicity tests with DSS (synthetic anionic detergent of the linear alkylate sulphonate type) on groups of grass shrimp, primarily Palaemonetes pugio (Holthius), collected from June 1972 through April 1974 from the same area of Galveston Bay, Texas. An attempt was made to determine the seasonal variation in the resistance of these crustaceans to DSS and the response of the organisms to DSS after time in the laboratory. The data indicate that grass shrimp tolerated substantial concentrations of DSS in comparison to marine fish and crustaceans. Grass shrimp are most tolerant to DDS during spring and summer and least tolerant during the winter months. The data demonstrated that organism health declined with increasing time in the laboratory. Although the grass shrimp exhibited 100% survival and behaved in a normal fashion during a holding period of months they were less resistant to DSS after being held only two weeks. The results suggest that the best procedures to follow regarding toxicity testing of aquatic organisms would be to begin experiments as soon as possible after collection. Ideally, the testing would be performed under temperature and salinity parameters similar to field conditions so that acclimation times could be as short as possible.

  5. Stylicins, a new family of antimicrobial peptides from the Pacific blue shrimp Litopenaeus stylirostris.

    Science.gov (United States)

    Rolland, J L; Abdelouahab, M; Dupont, J; Lefevre, F; Bachère, E; Romestand, B

    2010-03-01

    The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenaeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pI=5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containing 13 cysteine residues. The recombinant Ls-Stylicin1 has been isolated in both monomeric and dimeric forms. Both display strong antifungal activity against Fusarium oxysporum (1.25 microMshrimp, but lower antimicrobial activity against Gram (-) bacteria, Vibrio sp. (40 microMshrimp is thought to be the first member of a shrimp antimicrobial peptide family, which we termed stylicins. PMID:20061030

  6. 76 FR 18157 - Certain Frozen Warmwater Shrimp From Brazil, India, and Thailand: Notice of Initiation of...

    Science.gov (United States)

    2011-04-01

    ...., Ltd Shing Fu Seaproducts Development Co Siam Food Supply Co., Ltd Siam Intersea Co., Ltd Siam Marine... administrative review of the order on shrimp from India with respect to Vaibhav Sea Foods because in the 2004... Coreline Exports Corlim Marine Exports Pvt. Ltd Damco India Private Devi Fisheries Limited Devi Marine...

  7. 75 FR 17693 - Certain Frozen Warmwater Shrimp from Brazil, India, and Thailand: Notice of Initiation of...

    Science.gov (United States)

    2010-04-07

    ... Food Supply Co., Ltd Siam Intersea Co., Ltd Siam Marine Products Co. Ltd Siam Marine Frozen Foods Co... India Private Devi Fisheries Limited Devi Marine Food Exports Private Ltd./Kader Exports Private Limited... Foods, and Ananda Aqua Applications. See Certain Frozen Warmwater Shrimp From India: Preliminary...

  8. Bioencapsulation of Two Different Vibrio Species in Nauplii of the Brine Shrimp (Artemia franciscana)

    OpenAIRE

    Gomez-Gil, Bruno; Herrera-Vega, Maria A.; Abreu-Grobois, F. Alberto; Roque, Ana

    1998-01-01

    Two groups of nauplii from the brine shrimp (Artemia franciscana) were enriched with different bacteria, and the dynamics of bacterial uptake by the nauplii were observed. This study showed that the efficiency of Artemia nauplii in bioencapsulating bacteria strongly depends on the type of bacteria used, time of exposure, and status (live or dead) of the bacteria.

  9. White spot syndrome virus molecular epidemiology: relation with shrimp farming and disease outbreaks

    NARCIS (Netherlands)

    Tran Thi Tuyet, H.

    2012-01-01

    White spot syndrome virus (WSSV), the causative agent of white spot disease (WSD), has been responsible for most shrimp production losses around the world since the early 1990s. Previous research has focused mainly on the characterization of WSSV genomic variation to gain a better insight in the evo

  10. Culturable fungal diversity of shrimp Litopenaeus vannamei boone from breeding farms in Brazil

    Directory of Open Access Journals (Sweden)

    Lidiane Roberta Cruz da Silva

    2011-03-01

    Full Text Available Litopenaeus vannamei, which is the most common shrimp species cultivated in the northeast of Brazil, is very susceptible to microbial diseases, and this consequently affects productivity. There are reports of bacteria, viruses and protozoa in these shrimp, but not fungi. This study aims to isolate and identify fungi present in shrimp Litopenaeus vannamei, and in their nursery waters, at two breeding farms in Brazil. The pathogenic potential of the isolates was assessed through the qualitative detection of proteases and aflatoxin B production. The 146 isolated fungi comprised 46 species. Aspergillus, Penicillium and Furarium were the three most relevant genera and Aspergillus flavus was the predominant species with a total of 33 isolates. Most of the isolated species are known as potentially pathogenic to humans and other animals. Eighteen isolates of A. flavus and two of A. parasiticus were able to produce aflatoxin B and 33 out of the 46 species produced protease, indicating that these fungi may also become pathogenic to shrimp and their consumers.

  11. Iron oxide deposits associated with the ectosymbiotic bacteria in the hydrothermal vent shrimp Rimicaris exoculata

    Directory of Open Access Journals (Sweden)

    P. Compère

    2008-09-01

    Full Text Available The Rimicaris exoculata shrimp is considered as a primary consumer that dominates the fauna of most Mid-Atlantic Ridge (MAR hydrothermal ecosystems. These shrimps harbour in their gill chambers an important ectosymbiotic community of chemoautotrophic bacteria associated with iron oxide deposits. The structure and elemental composition of the mineral concretions associated with these bacteria have been investigated by using LM, ESEM, TEM STEM and EDX microanalyses. The nature of the iron oxides in shrimps obtained from the Rainbow vent field has also been determined by Mössbauer spectroscopy. This multidisciplinary approach has revealed that the three layers of mineral crust in the Rimicaris exoculata shrimps consist of large concretions formed by aggregated nanoparticles of two-line ferrihydrite and include other minor elements as Si, Ca, Mg, S and P, probably present as silicates cations, sulphates or phosphates respectively that may contribute to stabilise the ferrihydrite form of iron oxides. TEM-observations on the bacteria have revealed their close interactions with these minerals. Abiotic and biotic precipitation could occur within the gill chamber of Rimicaris exoculata, suggesting the biologically-mediated formation of the iron oxide deposits. The difference of the bacterial density in the three-mineral crust layers could be correlated to the importance of the iron oxide concretions and suggest that the first mineral particles precipitates on the lower layer which could be considered as the most likely location of iron-oxidizing bacteria.

  12. Concentration of metals in shrimps and crabs from Thane-Bassein creek system, Maharashtra

    Digital Repository Service at National Institute of Oceanography (India)

    Krishnamurti, A; Nair, V.R.

    Levels of Cu, Zn, Cd, Pb and Ni in shrimps and crabs from Thane-Bassein creek system, Maharashtra, India were evaluated for a period of one year and reported as baseline for future monitoring of this vital ecosystem. The pattern of concentration...

  13. EXTRACTION OF ASTAXANTHIN ESTERS FROM SHRIMP WASTE BY CHEMICAL AND MICROBIAL METHODS

    Directory of Open Access Journals (Sweden)

    A. Khanafari, A. Saberi, M. Azar, Gh. Vosooghi, Sh. Jamili, B. Sabbaghzadeh

    2007-04-01

    Full Text Available The carotenoid pigments specifically astaxanthin has many significant applications in food, pharmaceutical and cosmetic industries. The goal of this research was the extraction of Astaxanthin from a certain Persian Gulf shrimp species waste (Penaeus semisulcatus, purification and identification of the pigment by chemical and microbial methods. Microbial fermentation was obtained by inoculation of two Lactobacillus species Lb. plantarum and Lb. acidophilus in the medium culture containing shrimp waste powder by the intervention of lactose sugar, yeast extract, the composition of Both and the coolage (-20oC. The carotenoids were extracted by an organic solvent system. After purification of astaxanthin with the thin layer chromatography method by spectrophotometer, NMR and IR analysis the presence of astaxanthin esters was recognized in this specific species of Persian Gulf shrimp. Results obtained from this study showed that the coolage at –20 oC not only does not have an amplifying effect on the production of astaxanthin but also slightly reduces this effect. Also the effect of intervention of lactose sugar showed more effectiveness in producing astaxanthin than yeast extract or more than with the presence of both. The results also indicated that there is not much difference in the ability of producing the pigment by comparing both Lb. plantarum and Lb. acidophillus. Also results showed the microbial method of extraction of astaxanthin is more effective than chemical method. The pigment extracted from certain amount of shrimp powder, 23.128 mg/g, was calculated.

  14. Effects of Gamma Irradiation on Quality in the Processing of Low Salted and Fermented Shrimp

    International Nuclear Information System (INIS)

    Irradiation technology was applied to develop low salted and fermented shrimp that has better sensory quality and a longer shelf-life without any food additives. Different levels of salt (10, 15, and 20%, w/w) were added to the salted and fermented shrimp and the samples were irradiated at 0, 2.5, 5.0, 7.5, and 10.0 kGy with a gamma source (Co-60). Proximate composition, salinity, water activity (a), pH, total bacterial count, and general acceptance were analyzed during fermentation at 15 degrees after irradiation. The proximate analysis, salinity, and a were not affected by gamma irradiation during fermentation. However, pH and total bacteria, as well as sensory evaluation, were changed variously with processing conditions such as sodium chloride concentration and irradiation dose. The combinations of 15% salt concentration with 10 kGy irradiation dose and 20% with 5 kGy or above were effective for shelf-life enhancement of the salted and fermented shrimp by adequate suppression of microorganisms during fermentation at 15 degrees. The results showed that the sensory quality of the sample was maintained up to 10 weeks after fermentation. Therefore, it was considered that gamma irradiation was effective in processing low salted and fermented shrimp and extending their shelf-life without adding any food additives

  15. ACUTE AND CHRONIC TOXICITY OF BREVETOXIN TO OYSTERS AND GRASS SHRIMP

    Science.gov (United States)

    Walker, Calvin C., James T. Winstead, Steven S. Foss, Janis C. Kurtz, James Watts, Jeanne E. Scott and William S. Fisher. In press. Acute and Chronic Toxicity of Brevetoxin to Oysters and Grass Shrimp (Abstract). To be presented at the SETAC Fourth World Congress, 14-18 November ...

  16. Study on shrimp waste water and vermicompost as a nutrient source for bell peppers

    Science.gov (United States)

    The aquaculture industry generates significant nutrient-rich wastewater that is released into streams and rivers causing environmental concern. The objective of this controlled environment study was to evaluate the effect of waste shrimp water (SW), vermicompost (VC), at rates of 10%, 20%, 40%, and ...

  17. The Potential Use of Electricity to Control Burrowing Shrimp in Oyster Aquaculture Beds

    Science.gov (United States)

    Thalassinid shrimp cause significant problems for oyster aquaculture in the Pacific Northwest (USA) where oysters succumb to the physical disruption of the sediment by the burrowing activity of these animals. While electrofishing is a commonly used technique to capture fish and some invertebrates i...

  18. Distribution of bacteria in frozen shrimps and their decontamination by gamma irradiation

    International Nuclear Information System (INIS)

    Six samples of frozen shrimps were obtained through importing company mainly from South-east Asian countries. Five samples of frozen shrimps contained 1.5 x 105 to 6.2 x 106 per gram of total aerobic bacteria, and 4 samples contained 1.4 x 102 to 2.2 x 103 per gram of coliforms, which are higher contamination than Japanese standard of hygenic level for frozen food products (total counts below 105 per gram and no coliforms). The dominant bacteria in total counts were consisted with psychrotrophic Moraxella, Arthrobacter, Micrococcus, Acinetobacter, Flavobacterium, Corynebacterium, Pseudomonas and etc, in all kinds of frozen shrimps. Salmonellae or enteric Vibrio were could not isolated by ordinary isolation methods, and there were isolated many strains of Proteus mirabilis instead of these bacteria. Necessary dose of gamma irradiation for decontamination of bacteria to hygenic level was decided as 3 ∼ 5 kGy from the inactivation curves of total bacteria, coliforms, Proteus mirabilis and Salmonella typhymurium in frozen shrimps. Off-odor was suppressed by frozen condition of irradiation. (author)

  19. Distribution of bacteria in frozen shrimps and their decontamination by gamma irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Ito, Hitoshi; Adulyatham, P.; Ishigaki, Isao

    1987-11-01

    Six samples of frozen shrimps were obtained through importing company mainly from South-east Asian countries. Five samples of frozen shrimps contained 1.5 x 10/sup 5/ to 6.2 x 10/sup 6/ per gram of total aerobic bacteria, and 4 samples contained 1.4 x 10/sup 2/ to 2.2 x 10/sup 3/ per gram of coliforms, which are higher contamination than Japanese standard of hygenic level for frozen food products (total counts below 10/sup 5/ per gram and no coliforms). The dominant bacteria in total counts were consisted with psychrotrophic Moraxella, Arthrobacter, Micrococcus, Acinetobacter, Flavobacterium, Corynebacterium, Pseudomonas and etc, in all kinds of frozen shrimps. Salmonellae or enteric Vibrio were could not isolated by ordinary isolation methods, and there were isolated many strains of Proteus mirabilis instead of these bacteria. Necessary dose of gamma irradiation for decontamination of bacteria to hygenic level was decided as 3 approx. 5 kGy from the inactivation curves of total bacteria, coliforms, Proteus mirabilis and Salmonella typhymurium in frozen shrimps. Off-odor was suppressed by frozen condition of irradiation.

  20. Effect of gamma radiation on microbiological quality of shrimp paste (Kapi)

    International Nuclear Information System (INIS)

    Seven brands of shrimp paste (Kapi) sold in supermarkets were investigated for microbiological quality and quantity of salt. The effect of gamma radiation at dose 1 to 6 kGy on microbiological quality and on sensory quality of shrimp paste were also evaluated. Total viable bacterial counts of surveyed samples ranged from 1.20 x 104 to 4.00 x 105 colony forming unit (cfu) per gram and no detectable number of Enterobacteriaceae, coliforms, Faecal coliforms, Escherichia coil, Salmonella, Vibrio parahaemolyticus and Staphylococcus aureus was found. The most probable number (MPN) per gram of Clostridium perfringens is ranged from 9 to 240. Percentage of salt in surveyed shrimp pastes was rather high (ranged from 19.08 to 25.08). Radiation with 4 kGy resulted in 2 log cycles reduction of total viable bacterial counts and 2-4 log cycles reduction of halophilic bacteria, but no decrease in the most probable number per gram of CI. perfringens. A dose of 6 kGy gamma radiation was adequate for decreasing the MPN per gram of C. perfringens. No significant change in sensory scores for odour and flavor was observed in irradiated shrimp paste. Only in irradiated at dose of 6 kGy was the significant change in colour observed