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Sample records for chiiki tekigo gata

  1. Research cooperation project in fiscal 2001. Research cooperation on putting into practical use of district complying type photovoltaic power generation system; 2001 nendo kenkyu kyoryoku jigyo. Chiiki tekigo gata taiyoko hatsuden system tou no jitsuyoka ni kansuru kenkyu kyoryoku

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    Research cooperation has been performed with an intention of establishing technologies to develop a design program for photovoltaic power generation systems suitable for the diverse natural and social conditions in China, the performance inspections and quality enhancement thereon. This paper summarizes the achievements in fiscal 2001. For installing the photovoltaic power generation system, procurement, transportation and installation were carried out in Gansu Province for a 8.4 kW integrated system and in Qinghai Province for 10 kW and 12 kW integrated systems. Visits were made to the sites to confirm the operating conditions, and maintenance and management conditions of the systems having been installed to date. With regard to performance evaluation devices of the cells and modules, some pieces of the equipment were repaired and re-adjusted, and training for using them was executed. Eight persons have visited Japan from China and participated in the training in Japan for utilization and operation of the photovoltaic power generation systems for the persons involved in the system operation. In addition, in order to determine the future information exchange directions, workshops were held with PV experts and manufacturers participating from Japan to exchange items of information. (NEDO)

  2. Pancreas-specific deletion of mouse Gata4 and Gata6 causes pancreatic agenesis

    Science.gov (United States)

    Xuan, Shouhong; Borok, Matthew J.; Decker, Kimberly J.; Battle, Michele A.; Duncan, Stephen A.; Hale, Michael A.; Macdonald, Raymond J.; Sussel, Lori

    2012-01-01

    Pancreatic agenesis is a human disorder caused by defects in pancreas development. To date, only a few genes have been linked to pancreatic agenesis in humans, with mutations in pancreatic and duodenal homeobox 1 (PDX1) and pancreas-specific transcription factor 1a (PTF1A) reported in only 5 families with described cases. Recently, mutations in GATA6 have been identified in a large percentage of human cases, and a GATA4 mutant allele has been implicated in a single case. In the mouse, Gata4 and Gata6 are expressed in several endoderm-derived tissues, including the pancreas. To analyze the functions of GATA4 and/or GATA6 during mouse pancreatic development, we generated pancreas-specific deletions of Gata4 and Gata6. Surprisingly, loss of either Gata4 or Gata6 in the pancreas resulted in only mild pancreatic defects, which resolved postnatally. However, simultaneous deletion of both Gata4 and Gata6 in the pancreas caused severe pancreatic agenesis due to disruption of pancreatic progenitor cell proliferation, defects in branching morphogenesis, and a subsequent failure to induce the differentiation of progenitor cells expressing carboxypeptidase A1 (CPA1) and neurogenin 3 (NEUROG3). These studies address the conserved and nonconserved mechanisms underlying GATA4 and GATA6 function during pancreas development and provide a new mouse model to characterize the underlying developmental defects associated with pancreatic agenesis. PMID:23006325

  3. Transcription factors GATA-4 and GATA-6 in normal and neoplastic human gastrointestinal mucosa

    Directory of Open Access Journals (Sweden)

    Mäki Markku

    2008-04-01

    Full Text Available Abstract Background Human gastrointestinal mucosa regenerates vigorously throughout life, but the factors controlling cell fate in mature mucosa are poorly understood. GATA transcription factors direct cell proliferation and differentiation in many organs, and are implicated in tumorigenesis. GATA-4 and GATA-6 are considered crucial for the formation of murine gastrointestinal mucosa, but their role in human gastrointestinal tract remains unexplored. We studied in detail the expression patterns of these two GATA factors and a GATA-6 down-stream target, Indian hedgehog (Ihh, in normal human gastrointestinal mucosa. Since these factors are considered important for proliferation and differentiation, we also explored the possible alterations in their expression in gastrointestinal neoplasias. The expression of the carcinogenesis-related protein Indian hedgehog was also investigated in comparison to GATA factors. Methods Samples of normal and neoplastic gastrointestinal tract from children and adults were subjected to RNA in situ hybridization with 33P labelled probes and immunohistochemistry, using an avidin-biotin immunoperoxidase system. The pathological tissues examined included samples of chronic and atrophic gastritis as well as adenomas and adenocarcinomas of the colon and rectum. Results GATA-4 was abundant in the differentiated epithelial cells of the proximal parts of the gastrointestinal tract but was absent from the distal parts. In contrast, GATA-6 was expressed throughout the gastrointestinal epithelium, and in the distal gut its expression was most intense at the bottom of the crypts, i.e. cells with proliferative capacity. Both factors were also present in Barrett's esophagus and metaplasia of the stomach. GATA-6 expression was reduced in colon carcinoma. Ihh expression overlapped with that of GATA-6 especially in benign gastrointestinal neoplasias. Conclusion The results suggest differential but overlapping functions for GATA-4 and

  4. Achievement report (2/2) on R and D in fiscal 1999 on environment adaptive next-generation supersonic propulsion system. R and D of environment adaptive next-generation supersonic propulsion engine; 1999 nendo seika hokokusho. Kankyo tekigo gata jisedai choonsoku suishin system no kenkyu kaihatsu - Kankyo tekigo gata jisedai choonsokuki you enjin kaihatsu (2/2)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-12-01

    With an objective to develop an environment adaptive next-generation supersonic propulsion system, research and development have been performed on noise reducing technologies, NOx exhaust reducing technologies, CO2 exhaust reducing technologies, and environment adaptive engine system technologies. This paper summarizes the achievements in fiscal 1999. In developing the CO2 exhaust reducing technologies, discussions were given on the following items as the researches on a technology to apply three-dimensional fiber-reinforced materials to large structures: application of metal-based composite materials (MMC) to the fan rotor; application of CMC, TiAl materials to turbine structural materials; TiAl shroud support, CMC shroud, high load turbine cascades supported by the composite materials, and application of the CMC, TiAl materials to the gas exhaust nozzle. In developing the environment adaptive engine system technologies, flows were established to judge the achievement of the project target of noise reduction, NOx exhaust reduction and CO2 exhaust suppression. Furthermore, in the demonstration and research of the engine integration, the setting was conducted on the engine test schedule, and so was the primary setting for criteria on parts to be assembled into the engine. (NEDO)

  5. Hypermethylation of the GATA binding protein 4 (GATA4) promoter in Chinese pediatric acute myeloid leukemia

    International Nuclear Information System (INIS)

    Tao, Yan-Fang; Fang, Fang; Hu, Shao-Yan; Lu, Jun; Cao, Lan; Zhao, Wen-Li; Xiao, Pei-Fang; Li, Zhi-Heng; Wang, Na-Na; Xu, Li-Xiao; Du, Xiao-Juan; Sun, Li-Chao; Li, Yan-Hong; Li, Yi-Ping; Xu, Yun-Yun; Ni, Jian; Wang, Jian; Feng, Xing; Pan, Jian

    2015-01-01

    Acute myeloid leukemia (AML) is the second-most common form of leukemia in children. Aberrant DNA methylation patterns are a characteristic feature of AML. GATA4 has been suggested to be a tumor suppressor gene regulated by promoter hypermethylation in various types of human cancers although the expression and promoter methylation of GATA4 in pediatric AML is still unclear. Transcriptional expression levels of GATA4 were evaluated by semi-quantitative and real-time PCR. Methylation status was investigated by methylation-specific PCR (MSP) and bisulfate genomic sequencing (BGS). The prognostic significance of GATA4 expression and promoter methylation was assessed in 105 cases of Chinese pediatric acute myeloid leukemia patients with clinical follow-up records. MSP and BGS analysis showed that the GATA4 gene promoter is hypermethylated in AML cells, such as the HL-60 and MV4-11 human myeloid leukemia cell lines. 5-Aza treatment significantly upregulated GATA4 expression in HL-60 and MV4-11 cells. Aberrant methylation of GATA4 was observed in 15.0 % (3/20) of the normal bone marrow control samples compared to 56.2 % (59/105) of the pediatric AML samples. GATA4 transcript levels were significantly decreased in AML patients (33.06 ± 70.94; P = 0.011) compared to normal bone marrow/idiopathic thrombocytopenic purpura controls (116.76 ± 105.39). GATA4 promoter methylation was correlated with patient leukocyte counts (WBC, white blood cells) (P = 0.035) and minimal residual disease MRD (P = 0.031). Kaplan-Meier survival analysis revealed significantly shorter overall survival time in patients with GATA4 promoter methylation (P = 0.014). Epigenetic inactivation of GATA4 by promoter hypermethylation was observed in both AML cell lines and pediatric AML samples; our study implicates GATA4 as a putative tumor suppressor gene in pediatric AML. In addition, our findings imply that GATA4 promoter methylation is correlated with WBC and MRD. Kaplan-Meier survival analysis

  6. The ubiquitin ligase SCFFBXW7α promotes GATA3 degradation.

    Science.gov (United States)

    Song, Nan; Cao, Cheng; Tang, Yiman; Bi, Liyuan; Jiang, Yong; Zhou, Yongsheng; Song, Xin; Liu, Ling; Ge, Wenshu

    2018-03-01

    GATA3 is a key transcription factor in cell fate determination and its dysregulation has been implicated in various types of malignancies. However, how the abundance and function of GATA3 are regulated remains unclear. Here, we report that GATA3 is physically associated with FBXW7α, and FBXW7α destabilizes GATA3 through assembly of a SKP1-CUL1-F-box E3 ligase complex. Importantly, we showed that FBXW7α promotes GATA3 ubiquitination and degradation in a GSK3 dependent manner. Furthermore, we demonstrated that FBXW7α inhibits breast cancer cells survival through destabilizing GATA3, and the expression level of FBXW7α is negatively correlated with that of GATA3 in breast cancer samples. This study indicated that FBXW7α is a critical negative regulator of GATA3 and revealed a pathway for the maintenance of GATA3 abundance in breast cancer cells. © 2017 Wiley Periodicals, Inc.

  7. Regulatory network of GATA3 in pediatric acute lymphoblastic leukemia.

    Science.gov (United States)

    Hou, Qianqian; Liao, Fei; Zhang, Shouyue; Zhang, Duyu; Zhang, Yan; Zhou, Xueyan; Xia, Xuyang; Ye, Yuanxin; Yang, Hanshuo; Li, Zhaozhi; Wang, Leiming; Wang, Xi; Ma, Zhigui; Zhu, Yiping; Ouyang, Liang; Wang, Yuelan; Zhang, Hui; Yang, Li; Xu, Heng; Shu, Yang

    2017-05-30

    GATA3 polymorphisms were reported to be significantly associated with susceptibility of pediatric B-lineage acute lymphoblastic leukemia (ALL), by impacting on GATA3 expression. We noticed that ALL-related GATA3 polymorphism located around in the tissue-specific enhancer, and significantly associated with GATA3 expression. Although the regulatory network of GATA3 has been well reported in T cells, the functional status of GATA3 is poorly understood in B-ALL. We thus conducted genome-wide gene expression association analyses to reveal expression associated genes and pathways in nine independent B-ALL patient cohorts. In B-ALL patients, 173 candidates were identified to be significantly associated with GATA3 expression, including some reported GATA3-related genes (e.g., ITM2A) and well-known tumor-related genes (e.g., STAT4). Some of the candidates exhibit tissue-specific and subtype-specific association with GATA3. Through overexpression and down-regulation of GATA3 in leukemia cell lines, several reported and novel GATA3 regulated genes were validated. Moreover, association of GATA3 expression and its targets can be impacted by SNPs (e.g., rs4894953), which locate in the potential GATA3 binding motif. Our findings suggest that GATA3 may be involved in multiple tumor-related pathways (e.g., STAT/JAK pathway) in B-ALL to impact leukemogenesis through epigenetic regulation.

  8. GATA-2 and GATA-3 regulate trophoblast-specific gene expression in vivo.

    NARCIS (Netherlands)

    G.T. Ma (Grace); M.E. Roth (Matthew); J.C. Groskopf (John); F.G. Grosveld (Frank); J.D. Engel (Douglas); D.I.H. Linzer (Daniel); F.Y. Tsai (Fong-Ying); S.H. Orkin (Stuart)

    1997-01-01

    textabstractWe previously demonstrated that the zinc finger transcription factors GATA-2 and GATA-3 are expressed in trophoblast giant cells and that they regulate transcription from the mouse placental lactogen I gene promoter in a transfected trophoblast cell line. We present evidence here that

  9. Regulatory network of GATA3 in pediatric acute lymphoblastic leukemia

    OpenAIRE

    Hou, Qianqian; Liao, Fei; Zhang, Shouyue; Zhang, Duyu; Zhang, Yan; Zhou, Xueyan; Xia, Xuyang; Ye, Yuanxin; Yang, Hanshuo; Li, Zhaozhi; Wang, Leiming; Wang, Xi; Ma, Zhigui; Zhu, Yiping; Ouyang, Liang

    2017-01-01

    GATA3 polymorphisms were reported to be significantly associated with susceptibility of pediatric B-lineage acute lymphoblastic leukemia (ALL), by impacting on GATA3 expression. We noticed that ALL-related GATA3 polymorphism located around in the tissue-specific enhancer, and significantly associated with GATA3 expression. Although the regulatory network of GATA3 has been well reported in T cells, the functional status of GATA3 is poorly understood in B-ALL. We thus conducted genome-wide gene...

  10. Overexpression of human GATA-1 and GATA-2 interferes with spine formation and produces depressive behavior in rats.

    Directory of Open Access Journals (Sweden)

    Miyeon Choi

    Full Text Available Functional consequences to which vertebrate GATA transcription factors contribute in the adult brain remain largely an open question. The present study examines how human GATA-1 and GATA-2 (hGATA-1 and hGATA-2 are linked to neuronal differentiation and depressive behaviors in rats. We investigated the effects of adeno-associated viral expression of hGATA-1 and hGATA-2 (AAV-hGATA1 and AAV-hGATA2 in the dentate gyrus (DG of the dorsal hippocampus on dendrite branching and spine number. We also examined the influence of AAV-hGATA1 and AAV-hGATA2 infusions into the dorsal hippocampus on rodent behavior in models of depression. Viral expression of hGATA-1 and hGATA-2 cDNA in rat hippocampal neurons impaired dendritic outgrowth and spine formation. Moreover, viral-mediated expression of hGATA-1 and hGATA-2 in the dorsal hippocampus caused depressive-like deficits in the forced swim test and learned helplessness models of depression, and decreased the expression of several synapse-related genes as well as spine number in hippocampal neurons. Conversely, shRNA knockdown of GATA-2 increased synapse-related gene expression, spine number, and dendrite branching. The results demonstrate that hGATA-1 and hGATA-2 expression in hippocampus is sufficient to cause depressive like behaviors that are associated with reduction in spine synapse density and expression of synapse-related genes.

  11. Pediatric MDS: GATA screen the germline.

    Science.gov (United States)

    Stieglitz, Elliot; Loh, Mignon L

    2016-03-17

    In this issue of Blood, Wlodarski and colleagues demonstrate that as many as 72% of adolescents diagnosed with myelodysplastic syndrome (MDS) and monosomy 7 harbor germline mutations in GATA2. Although pediatric MDS is a very rare diagnosis, occurring in 0.8 to 4 cases per million, Wlodarski et al screened >600 cases of primary or secondary MDS in children and adolescents who were enrolled in the European Working Group on MDS consortium over a period of 15 years. The overall frequency of germline GATA2 mutations in children with primary MDS was 7%, and 15% in those presenting with advanced disease. Notably, mutations in GATA2 were absent in patients with therapy-related MDS or acquired aplastic anemia.

  12. GATA transcription factors in testicular adrenal rest tumours

    Directory of Open Access Journals (Sweden)

    Manon Engels

    2017-11-01

    Full Text Available Testicular adrenal rest tumours (TARTs are benign adrenal-like testicular tumours that frequently occur in male patients with congenital adrenal hyperplasia. Recently, GATA transcription factors have been linked to the development of TARTs in mice. The aim of our study was to determine GATA expression in human TARTs and other steroidogenic tissues. We determined GATA expression in TARTs (n = 16, Leydig cell tumours (LCTs; n = 7, adrenal (foetal (n = 6 + adult (n = 10 and testis (foetal (n = 13 + adult (n = 8. We found testis-like GATA4, and adrenal-like GATA3 and GATA6 gene expressions by qPCR in human TARTs, indicating mixed testicular and adrenal characteristics of TARTs. Currently, no marker is available to discriminate TARTs from LCTs, leading to misdiagnosis and incorrect treatment. GATA3 and GATA6 mRNAs exhibited excellent discriminative power (area under the curve of 0.908 and 0.816, respectively, while immunohistochemistry did not. GATA genes contain several CREB-binding sites and incubation with 0.1 mM dibutyryl cAMP for 4 h stimulated GATA3, GATA4 and GATA6 expressions in a human foetal testis cell line (hs181.tes. Incubation of adrenocortical cells (H295RA with ACTH, however, did not induce GATA expression in vitro. Although ACTH did not dysregulate GATA expression in the only human ACTH-sensitive in vitro model available, our results do suggest that aberrant expression of GATA transcription factors in human TARTs might be involved in TART formation.

  13. GATA-1 directly regulates Nanog in mouse embryonic stem cells

    Energy Technology Data Exchange (ETDEWEB)

    Li, Wen-Zhong; Ai, Zhi-Ying [College of Life Sciences, Northwest A& F University, Yangling 712100 (China); Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A& F University, Yangling 712100 (China); Wang, Zhi-Wei [School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui 230027 (China); Chen, Lin-Lin [College of Life Sciences, Northwest A& F University, Yangling 712100 (China); Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A& F University, Yangling 712100 (China); Guo, Ze-Kun, E-mail: gzknwaf@126.com [College of Veterinary Medicine, Northwest A& F University, Yangling 712100 (China); Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A& F University, Yangling 712100 (China); Zhang, Yong, E-mail: zylabnwaf@126.com [College of Veterinary Medicine, Northwest A& F University, Yangling 712100 (China); Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A& F University, Yangling 712100 (China)

    2015-09-25

    Nanog safeguards pluripotency in mouse embryonic stem cells (mESCs). Insight into the regulation of Nanog is important for a better understanding of the molecular mechanisms that control pluripotency of mESCs. In a silico analysis, we identify four GATA-1 putative binding sites in Nanog proximal promoter. The Nanog promoter activity can be significantly repressed by ectopic expression of GATA-1 evidenced by a promoter reporter assay. Mutation studies reveal that one of the four putative binding sites counts for GATA-1 repressing Nanog promoter activity. Direct binding of GATA-1 on Nanog proximal promoter is confirmed by electrophoretic mobility shift assay and chromatin immunoprecipitation. Our data provide new insights into the expanded regulatory circuitry that coordinates Nanog expression. - Highlights: • The Nanog proximal promoter conceives functional element for GATA-1. • GATA-1 occupies the Nanog proximal promoter in vitro and in vivo. • GATA-1 transcriptionally suppresses Nanog.

  14. Analysis list: GATA3 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available GATA3 Blood,Breast,Neural + hg19 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/GATA3.1.tsv http:...//dbarchive.biosciencedbc.jp/kyushu-u/hg19/target/GATA3.5.tsv http://dbarchive.bioscie...ncedbc.jp/kyushu-u/hg19/target/GATA3.10.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/GATA3.Blood.tsv,http:...//dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/GATA3.Breast.tsv,http:/.../dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/GATA3.Neural.tsv http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/colo/Blood.gml,http:

  15. Dynamics of GATA1 binding and expression response in a GATA1-induced erythroid differentiation system

    Directory of Open Access Journals (Sweden)

    Deepti Jain

    2015-06-01

    Full Text Available During the maturation phase of mammalian erythroid differentiation, highly proliferative cells committed to the erythroid lineage undergo dramatic changes in morphology and function to produce circulating, enucleated erythrocytes. These changes are caused by equally dramatic alterations in gene expression, which in turn are driven by changes in the abundance and binding patterns of transcription factors such as GATA1. We have studied the dynamics of GATA1 binding by ChIP-seq and the global expression responses by RNA-seq in a GATA1-dependent mouse cell line model for erythroid maturation, in both cases examining seven progressive stages during differentiation. Analyses of these data should provide insights both into mechanisms of regulation (early versus late targets and the consequences in cell physiology (e.g., distinctive categories of genes regulated at progressive stages of differentiation. The data are deposited in the Gene Expression Omnibus, series GSE36029, GSE40522, GSE49847, and GSE51338.

  16. Combinatorial regulation of tissue specification by GATA and FOG factors

    Science.gov (United States)

    Chlon, Timothy M.; Crispino, John D.

    2012-01-01

    The development of complex organisms requires the formation of diverse cell types from common stem and progenitor cells. GATA family transcriptional regulators and their dedicated co-factors, termed Friend of GATA (FOG) proteins, control cell fate and differentiation in multiple tissue types from Drosophila to man. FOGs can both facilitate and antagonize GATA factor transcriptional regulation depending on the factor, cell, and even the specific gene target. In this review, we highlight recent studies that have elucidated mechanisms by which FOGs regulate GATA factor function and discuss how these factors use these diverse modes of gene regulation to control cell lineage specification throughout metazoans. PMID:23048181

  17. Protein-altering and regulatory genetic variants near GATA4 implicated in bicuspid aortic valve

    DEFF Research Database (Denmark)

    Yang, Bo; Zhou, Wei-Wu; Jiao, Jiao

    2017-01-01

    . We identify association with a noncoding variant 151 kb from the gene encoding the cardiac-specific transcription factor, GATA4, and near-significance for p.Ser377Gly in GATA4. GATA4 was interrupted by CRISPR-Cas9 in induced pluripotent stem cells from healthy donors. The disruption of GATA4...

  18. GATA3 expression in triple-negative breast cancers.

    Science.gov (United States)

    Byrne, David J; Deb, Siddhartha; Takano, Elena A; Fox, Stephen B

    2017-07-01

    GATA-binding protein 3 (GATA3) is a well-studied transcription factor found to be essential in the development of luminal breast epithelium and has been identified in a variety of tumour types, including breast and urothelial carcinomas, making it a useful immunohistochemistry marker in the diagnosis of both primary and metastatic disease. We investigated GATA3 protein expression in a 106 primary triple-negative breast carcinomas (100 basal-like, six non-basal-like) using Cell Marque mouse monoclonal anti-GATA3 (L50-823). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to quantify mRNA expression in 22 triple-negative breast cancers (TNBCs) (20 primary and two cell lines), four luminal (three primary and one cell line) and five human epidermal growth factor receptor 2 (HER2) (four primary and one cell line) amplified tumours. In 98 TNBCs where IHC was assessable, 47 (48%) had a 1+ or greater staining with 20 (21%) having high GATA3 expression when using a weighted scoring. Our study has demonstrated that GATA3 expression is common in primary triple-negative breast carcinomas. It also suggests that although GATA3 is an oestrogen receptor (ER) regulated gene, it still proves useful in differentiating between primary and metastatic tumours in patients with a history of breast cancer regardless of its molecular subtype. © 2017 John Wiley & Sons Ltd.

  19. VEGF and IHH rescue definitive hematopoiesis in Gata-4 and Gata-6-deficient murine embryoid bodies.

    Science.gov (United States)

    Pierre, Monique; Yoshimoto, Momoko; Huang, Lan; Richardson, Matthew; Yoder, Mervin C

    2009-09-01

    Murine embryonic stem cells can be differentiated into embryoid bodies (EBs), which serve as an in vitro model recapitulating many aspects of embryonic yolk sac hematopoiesis. Differentiation of embryonic stem cells deficient in either Gata-4 or Gata-6 results in EBs with disrupted visceral endoderm (VE). While lack of VE has detrimental effects on hematopoiesis in vivo, it is unclear whether lack of VE affects hematopoiesis in EBs. Therefore, we compared Gata-4 null (G4N) and Gata-6 null (G6N) EBs with wild-type EBs to assess their ability to commit to hematopoietic cells. EB VE formation was examined using cell-sorting techniques and analysis visceral endoderm gene expression. Hematopoietic progenitor potential of EBs cultured under various conditions was assessed using colony-forming assays. Definitive erythroid, granulocyte-macrophage, and mixed colonies were significantly reduced in G4N and G6N EBs compared to wild-type EBs. Vascular endothelial growth factor (VEGF) expression and secretion were also reduced in both G4N and G6N EBs, consistent with VE serving as a site of VEGF production. Addition of exogenous VEGF(165), to EB cultures completely rescued definitive colony-forming cells in G4N and G6N EBs. This rescue response could be blocked by addition of soluble Flk-1 Fc to EB cultures. Similarly, addition of exogenous Indian hedgehog to EB cultures also recovers the diminishment in definitive hematopoiesis in a reversible manner. These results suggest that the absence of VE in G4N and G6N EBs does not prevent emergence of definitive progenitors from EBs. However, the decreased level of VEGF and Indian hedgehog production in VE devoid G4N and G6N EBs attenuates definitive hematopoietic progenitor cell expansion.

  20. Gata4 expression in lateral mesoderm is downstream of BMP4 and isactivated directly by Forkhead and GATA transcription factors through adistal enhancer element

    Energy Technology Data Exchange (ETDEWEB)

    Rojas, Anabel; De Val, Sarah; Heidt, Analeah B.; Xu, Shan-Mei; Bristow, James; Black, Brian L.

    2005-05-20

    The GATA family of zinc-finger transcription factors plays key roles in the specification and differentiation of multiple cell types during development. GATA4 is an early regulator of gene expression during the development of endoderm and mesoderm, and genetic studies in mice have demonstrated that GATA4 is required for embryonic development.Despite the importance of GATA4 in tissue specification and differentiation, the mechanisms by which Gata4 expression is activated and the transcription factor pathways upstream of GATA4 remain largely undefined. To identify transcriptional regulators of Gata4 in the mouse,we screened conserved noncoding sequences from the mouse Gata4 gene for enhancer activity in transgenic embryos. Here, we define the regulation of a distal enhancer element from Gata4 that is sufficient to direct expression throughout the lateral mesoderm, beginning at 7.5 days of mouse embryonic development. The activity of this enhancer is initially broad but eventually becomes restricted to the mesenchyme surrounding the liver. We demonstrate that the function of this enhancer in transgenic embryos is dependent upon highly conserved Forkhead and GATA transcription factor binding sites, which are bound by FOXF1 and GATA4,respectively. Furthermore, the activity of the Gata4 lateral mesoderm enhancer is attenuated by the BMP antagonist Noggin, and the enhancer is not activated in Bmp4-null embryos. Thus, these studies establish that Gata4 is a direct transcriptional target of Forkhead and GATA transcription factors in the lateral mesoderm, and demonstrate that Gata4lateral mesoderm enhancer activation requires BMP4, supporting a model in which GATA4 serves as a downstream effector of BMP signaling in the lateral mesoderm.

  1. Differential developmental expression of transcription factors GATA-4 and GATA-6, their cofactor FOG-2 and downstream target genes in testicular carcinoma in situ and germ cell tumors

    DEFF Research Database (Denmark)

    Salonen, Jonna; Rajpert-De Meyts, E; Mannisto, Susanna

    2010-01-01

    Testicular germ cell cancer is the most common malignancy among young males. The pre-invasive precursor, carcinoma in situ testis (CIS), presumably originates from arrested and transformed fetal gonocytes. Given that GATA transcription factors have essential roles in embryonic and testicular deve...... development, we explored the expression of GATA-4, GATA-6, cofactor friend of GATA (FOG)-2, and downstream target genes during human testis development and addressed the question whether changes in this pathway may contribute to germ cell neoplasms....

  2. Whole genome duplications and expansion of the vertebrate GATA transcription factor gene family

    Directory of Open Access Journals (Sweden)

    Bowerman Bruce

    2009-08-01

    Full Text Available Abstract Background GATA transcription factors influence many developmental processes, including the specification of embryonic germ layers. The GATA gene family has significantly expanded in many animal lineages: whereas diverse cnidarians have only one GATA transcription factor, six GATA genes have been identified in many vertebrates, five in many insects, and eleven to thirteen in Caenorhabditis nematodes. All bilaterian animal genomes have at least one member each of two classes, GATA123 and GATA456. Results We have identified one GATA123 gene and one GATA456 gene from the genomic sequence of two invertebrate deuterostomes, a cephalochordate (Branchiostoma floridae and a hemichordate (Saccoglossus kowalevskii. We also have confirmed the presence of six GATA genes in all vertebrate genomes, as well as additional GATA genes in teleost fish. Analyses of conserved sequence motifs and of changes to the exon-intron structure, and molecular phylogenetic analyses of these deuterostome GATA genes support their origin from two ancestral deuterostome genes, one GATA 123 and one GATA456. Comparison of the conserved genomic organization across vertebrates identified eighteen paralogous gene families linked to multiple vertebrate GATA genes (GATA paralogons, providing the strongest evidence yet for expansion of vertebrate GATA gene families via genome duplication events. Conclusion From our analysis, we infer the evolutionary birth order and relationships among vertebrate GATA transcription factors, and define their expansion via multiple rounds of whole genome duplication events. As the genomes of four independent invertebrate deuterostome lineages contain single copy GATA123 and GATA456 genes, we infer that the 0R (pre-genome duplication invertebrate deuterostome ancestor also had two GATA genes, one of each class. Synteny analyses identify duplications of paralogous chromosomal regions (paralogons, from single ancestral vertebrate GATA123 and GATA456

  3. GATA transcription factors associate with a novel class of nuclear bodies in erythroblasts and megakaryocytes.

    NARCIS (Netherlands)

    A.G. Elefanty (Andrew); M. Antoniou (Michael); N. Custodio; M. Carmo-Fonseca; F.G. Grosveld (Frank)

    1996-01-01

    textabstractThe nuclear distribution of GATA transcription factors in murine haemopoietic cells was examined by indirect immunofluorescence. Specific bright foci of GATA-1 fluorescence were observed in erythroleukaemia cells and primary murine erythroblasts and megakaryocytes, in addition to diffuse

  4. Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation.

    Science.gov (United States)

    Chennupati, Vijaykumar; Veiga, Diogo Ft; Maslowski, Kendle M; Andina, Nicola; Tardivel, Aubry; Yu, Eric Chi-Wang; Stilinovic, Martina; Simillion, Cedric; Duchosal, Michel A; Quadroni, Manfredo; Roberts, Irene; Sankaran, Vijay G; MacDonald, H Robson; Fasel, Nicolas; Angelillo-Scherrer, Anne; Schneider, Pascal; Hoang, Trang; Allam, Ramanjaneyulu

    2018-04-02

    Ribosomal proteins (RP) regulate specific gene expression by selectively translating subsets of mRNAs. Indeed, in Diamond-Blackfan anemia and 5q- syndrome, mutations in RP genes lead to a specific defect in erythroid gene translation and cause anemia. Little is known about the molecular mechanisms of selective mRNA translation and involvement of ribosomal-associated factors in this process. Ribonuclease inhibitor 1 (RNH1) is a ubiquitously expressed protein that binds to and inhibits pancreatic-type ribonucleases. Here, we report that RNH1 binds to ribosomes and regulates erythropoiesis by controlling translation of the erythroid transcription factor GATA1. Rnh1-deficient mice die between embryonic days E8.5 and E10 due to impaired production of mature erythroid cells from progenitor cells. In Rnh1-deficient embryos, mRNA levels of Gata1 are normal, but GATA1 protein levels are decreased. At the molecular level, we found that RNH1 binds to the 40S subunit of ribosomes and facilitates polysome formation on Gata1 mRNA to confer transcript-specific translation. Further, RNH1 knockdown in human CD34+ progenitor cells decreased erythroid differentiation without affecting myelopoiesis. Our results reveal an unsuspected role for RNH1 in the control of GATA1 mRNA translation and erythropoiesis.

  5. GATA4 and GATA6 Knockdown During Luteinization Inhibits Progesterone Production and Gonadotropin Responsiveness in the Corpus Luteum of Female Mice.

    Science.gov (United States)

    Convissar, Scott M; Bennett, Jill; Baumgarten, Sarah C; Lydon, John P; DeMayo, Francesco J; Stocco, Carlos

    2015-12-01

    The surge of luteinizing hormone triggers the genomic reprogramming, cell differentiation, and tissue remodeling of the ovulated follicle, leading to the formation of the corpus luteum. During this process, called luteinization, follicular granulosa cells begin expressing a new set of genes that allow the resulting luteal cells to survive in a vastly different hormonal environment and to produce the extremely high amounts of progesterone (P4) needed to sustain pregnancy. To better understand the molecular mechanisms involved in the regulation of luteal P4 production in vivo, the transcription factors GATA4 and GATA6 were knocked down in the corpus luteum by crossing mice carrying Gata4 and Gata6 floxed genes with mice carrying Cre recombinase fused to the progesterone receptor. This receptor is expressed exclusively in granulosa cells after the luteinizing hormone surge, leading to recombination of floxed genes during follicle luteinization. The findings demonstrated that GATA4 and GATA6 are essential for female fertility, whereas targeting either factor alone causes subfertility. When compared to control mice, serum P4 levels and luteal expression of key steroidogenic genes were significantly lower in conditional knockdown mice. The results also showed that GATA4 and GATA6 are required for the expression of the receptors for prolactin and luteinizing hormone, the main luteotropic hormones in mice. The findings demonstrate that GATA4 and GATA6 are crucial regulators of luteal steroidogenesis and are required for the normal response of luteal cells to luteotropins. © 2015 by the Society for the Study of Reproduction, Inc.

  6. GATA Factor-Dependent Positive-Feedback Circuit in Acute Myeloid Leukemia Cells

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    Koichi R. Katsumura

    2016-08-01

    Full Text Available The master regulatory transcription factor GATA-2 triggers hematopoietic stem and progenitor cell generation. GATA2 haploinsufficiency is implicated in myelodysplastic syndrome (MDS and acute myeloid leukemia (AML, and GATA2 overexpression portends a poor prognosis for AML. However, the constituents of the GATA-2-dependent genetic network mediating pathogenesis are unknown. We described a p38-dependent mechanism that phosphorylates GATA-2 and increases GATA-2 target gene activation. We demonstrate that this mechanism establishes a growth-promoting chemokine/cytokine circuit in AML cells. p38/ERK-dependent GATA-2 phosphorylation facilitated positive autoregulation of GATA2 transcription and expression of target genes, including IL1B and CXCL2. IL-1β and CXCL2 enhanced GATA-2 phosphorylation, which increased GATA-2-mediated transcriptional activation. p38/ERK-GATA-2 stimulated AML cell proliferation via CXCL2 induction. As GATA2 mRNA correlated with IL1B and CXCL2 mRNAs in AML-M5 and high expression of these genes predicted poor prognosis of cytogenetically normal AML, we propose that the circuit is functionally important in specific AML contexts.

  7. Screening of 99 Danish patients with congenital heart disease for GATA4 mutations

    DEFF Research Database (Denmark)

    Zhang, Litu; Tümer, Zeynep; Jacobsen, Joes Ramsøe

    2006-01-01

    stenosis. We have screened 99 unrelated Danish patients with different CHD phenotypes to evaluate the prevalence of GATA4 mutations in CHD. No pathogenic mutations were found among the patients, suggesting that GATA4 mutations are relatively rare among CHD patients. Thus, the diagnostic importance of GATA4...

  8. B-GATA transcription factors - insights into their structure, regulation and role in plant development

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    Claus eSchwechheimer

    2015-02-01

    Full Text Available GATA transcription factors are evolutionarily conserved transcriptional regulators that recognize promoter elements with a G-A-T-A core sequence. In comparison to animal genomes, the GATA transcription factor family in plants is comparatively large with approximately 30 members. In spite of a long-standing interest of plant molecular biologists in GATA factors, only research conducted in the last years has led to reliable insights into their functions during plant development. Here, we review the current knowledge on B-GATAs, one of four GATA factor subfamilies from Arabidopsis thaliana. We show that B-GATAs can be subdivided based on structural features and their biological function into family members with a C-terminal LLM- (leucine-leucine-methionine domain or an N-terminal HAN- (HANABA TARANU domain. The paralogous GNC (GATA, NITRATE-INDUCIBLE, CARBON-METABOLISM INVOLVED and CGA1/GNL (CYTOKININ-INDUCED GATA1/GNC-LIKE are introduced as LLM-domain containing B-GATAs from Arabidopsis that control germination, greening, senescence and flowering time downstream from several growth regulatory signals including light and the hormones gibberellin, auxin, and cytokinin. Arabidopsis HAN and its monocot-specific paralogs from rice (NECK LEAF1, maize (TASSEL SHEATH1, and barley (THIRD OUTER GLUME are HAN-domain-containing B-GATAs with a predominant role in embryo development and floral development. We also review GATA23, a regulator of lateral root initiation from Arabidopsis, that is closely related to GNC and GNL but has a degenerate LLM-domain that is seemingly specific for the Brassicaceae family. The Brassicaceae-specific GATA23 together with the above-mentioned monocot-specific HAN-domain GATAs provide evidence that neofunctionalization of the B-GATAs was used during plant evolution to expand the functional repertoire of these transcription factors.

  9. GATA2 Deficiency and Epstein-Barr Virus Disease.

    Science.gov (United States)

    Cohen, Jeffrey I

    2017-01-01

    GATA2 is a transcription factor that binds to the promoter of hematopoietic genes. Mutations in one copy of the gene are associated with haploinsufficiency and reduced levels of protein. This results in reduced numbers of several cell types important for immune surveillance including dendritic cells, monocytes, CD4, and NK cells, as well as impaired NK cell function. Recently, GATA2 has been associated with several different presentations of severe Epstein-Barr virus (EBV) disease including primary infection requiring repeated hospitalizations, chronic active EBV disease, EBV-associated hydroa vacciniforme with hemophagocytosis, and EBV-positive smooth muscle tumors. EBV was found predominantly in B cells in each of the cases in which it was studied, unlike most cases of chronic active EBV disease in which the virus is usually present in T or NK cells. The variety of EBV-associated diseases seen in patients with GATA2 deficiency suggest that additional forms of severe EBV disease may be found in patients with GATA2 deficiency in the future.

  10. GATA2 Deficiency and Epstein–Barr Virus Disease

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    Jeffrey I. Cohen

    2017-12-01

    Full Text Available GATA2 is a transcription factor that binds to the promoter of hematopoietic genes. Mutations in one copy of the gene are associated with haploinsufficiency and reduced levels of protein. This results in reduced numbers of several cell types important for immune surveillance including dendritic cells, monocytes, CD4, and NK cells, as well as impaired NK cell function. Recently, GATA2 has been associated with several different presentations of severe Epstein–Barr virus (EBV disease including primary infection requiring repeated hospitalizations, chronic active EBV disease, EBV-associated hydroa vacciniforme with hemophagocytosis, and EBV-positive smooth muscle tumors. EBV was found predominantly in B cells in each of the cases in which it was studied, unlike most cases of chronic active EBV disease in which the virus is usually present in T or NK cells. The variety of EBV-associated diseases seen in patients with GATA2 deficiency suggest that additional forms of severe EBV disease may be found in patients with GATA2 deficiency in the future.

  11. GATA3 mutation in a family with hypoparathyroidism, deafness and renal dysplasia syndrome.

    Science.gov (United States)

    Zhu, Zi-Yang; Zhou, Qiao-Li; Ni, Shi-Ning; Gu, Wei

    2014-08-01

    The hypoparathyroidism, deafness and renal dysplasia (HDR) syndrome is an autosomal dominant disorder primarily caused by GATA3 gene mutation. We report here a case that both of a Chinese boy and his father had HDR syndrome which caused by a novel mutation of GATA3. Polymerase chain reaction and DNA sequencing was performed to detect the exons of the GATA3 gene for mutation analysis. Sequence analysis of GATA3 revealed a heterozygous nonsense mutation in this family: a mutation of GATA3 at exon 2 (c.515C >A) that resulted in a premature stop at codon 172 (p.S172X) with a loss of two zinc finger domains. We identified a novel nonsense mutation which will expand the spectrum of HDR-associated GATA3 mutations.

  12. GATA3 expression in advanced breast cancer: prognostic value and organ-specific relapse.

    Science.gov (United States)

    McCleskey, Brandi C; Penedo, Thuy L; Zhang, Kui; Hameed, Omar; Siegal, Gene P; Wei, Shi

    2015-11-01

    GATA3 is a transcription factor regulating luminal cell differentiation in the mammary glands and has been implicated in the luminal types of breast carcinoma. The prognostic significance of GATA3 in breast cancer remains controversial. In this study, we assessed the prognostic value of the molecule in a subset of 62 advanced breast cancers and 10 control breast cancers (no metastasis after follow-up). GATA3 expression levels in luminal tumors of advanced stage were significantly higher than that of the human epidermal growth factor receptor 2 (HER2) subtype and triple-negative carcinomas, as expected, but were similar to those of the luminal controls. Furthermore, 88% of nonluminal tumors showed variable GATA3 expression, for which the HER2 subtype had significantly higher GATA3 expression than that of the triple-negative carcinomas. Interestingly, GATA3 levels were significantly lower in carcinomas with lung relapse compared to those with metastatic recurrence to other organs, thus reflecting the findings in animal models. No significant difference was observed between tumors with bone relapse and those metastasized to nonskeletal sites. Moreover, high GATA3 expression was significantly associated with favorable relapse-free survival and overall survival. These findings suggest that GATA3 may not act solely as a luminal differentiation marker, and further uncovering the molecular pathways by which GATA3 regulates the downstream targets will be crucial to our understanding of breast cancer dissemination. Copyright© by the American Society for Clinical Pathology.

  13. Generation of mice harbouring a conditional loss-of-function allele of Gata6

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    Duncan Stephen A

    2006-04-01

    Full Text Available Abstract The zinc finger transcription factor GATA6 is believed to have important roles in the development of several organs including the liver, gastrointestinal tract and heart. However, analyses of the contribution of GATA6 toward organogenesis have been hampered because Gata6-/- mice fail to develop beyond gastrulation due to defects in extraembryonic endoderm function. We have therefore generated a mouse line harbouring a conditional loss-of-function allele of Gata6 using Cre/loxP technology. LoxP elements were introduced into introns flanking exon 2 of the Gata6 gene by homologous recombination in ES cells. Mice containing this altered allele were bred to homozygosity and were found to be viable and fertile. To assess the functional integrity of the loxP sites and to confirm that we had generated a Gata6 loss-of-function allele, we bred Gata6 'floxed' mice to EIIa-Cre mice in which Cre is ubiquitously expressed, and to Villin-Cre mice that express Cre in the epithelial cells of the intestine. We conclude that we have generated a line of mice in which GATA6 activity can be ablated in a cell type specific manner by expression of Cre recombinase. This line of mice can be used to establish the role of GATA6 in regulating embryonic development and various aspects of mammalian physiology.

  14. Continued expression of GATA3 is necessary for cochlear neurosensory development.

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    Jeremy S Duncan

    Full Text Available Hair cells of the developing mammalian inner ear are progressively defined through cell fate restriction. This process culminates in the expression of the bHLH transcription factor Atoh1, which is necessary for differentiation of hair cells, but not for their specification. Loss of several genes will disrupt ear morphogenesis or arrest of neurosensory epithelia development. We previously showed in null mutants that the loss of the transcription factor, Gata3, results specifically in the loss of all cochlear neurosensory development. Temporal expression of Gata3 is broad from the otic placode stage through the postnatal ear. It therefore remains unclear at which stage in development Gata3 exerts its effect. To better understand the stage specific effects of Gata3, we investigated the role of Gata3 in cochlear neurosensory specification and differentiation utilizing a LoxP targeted Gata3 line and two Cre lines. Foxg1(Cre∶Gata3(f/f mice show recombination of Gata3 around E8.5 but continue to develop a cochlear duct without differentiated hair cells and spiral ganglion neurons. qRT-PCR data show that Atoh1 was down-regulated but not absent in the duct whereas other hair cell specific genes such as Pou4f3 were completely absent. In addition, while Sox2 levels were lower in the Foxg1(Cre:Gata3(f/f cochlea, Eya1 levels remained normal. We conclude that Eya1 is unable to fully upregulate Atoh1 or Pou4f3, and drive differentiation of hair cells without Gata3. Pax2-Cre∶Gata3(f/f mice show a delayed recombination of Gata3 in the ear relative to Foxg1(Cre:Gata3(f/f . These mice exhibited a cochlear duct containing patches of partially differentiated hair cells and developed only few and incorrectly projecting spiral ganglion neurons. Our conditional deletion studies reveal a major role of Gata3 in the signaling of prosensory genes and in the differentiation of cochlear neurosenory cells. We suggest that Gata3 may act in combination with Eya1, Six1, and

  15. GATA4 Is Sufficient to Establish Jejunal Versus Ileal Identity in the Small IntestineSummary

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    Cayla A. Thompson

    2017-05-01

    Full Text Available Background & Aims: Patterning of the small intestinal epithelium along its cephalocaudal axis establishes three functionally distinct regions: duodenum, jejunum, and ileum. Efficient nutrient assimilation and growth depend on the proper spatial patterning of specialized digestive and absorptive functions performed by duodenal, jejunal, and ileal enterocytes. When enterocyte function is disrupted by disease or injury, intestinal failure can occur. One approach to alleviate intestinal failure would be to restore lost enterocyte functions. The molecular mechanisms determining regionally defined enterocyte functions, however, are poorly delineated. We previously showed that GATA binding protein 4 (GATA4 is essential to define jejunal enterocytes. The goal of this study was to test the hypothesis that GATA4 is sufficient to confer jejunal identity within the intestinal epithelium. Methods: To test this hypothesis, we generated a novel Gata4 conditional knock-in mouse line and expressed GATA4 in the ileum, where it is absent. Results: We found that GATA4-expressing ileum lost ileal identity. The global gene expression profile of GATA4-expressing ileal epithelium aligned more closely with jejunum and duodenum rather than ileum. Focusing on jejunal vs ileal identity, we defined sets of jejunal and ileal genes likely to be regulated directly by GATA4 to suppress ileal identity and promote jejunal identity. Furthermore, our study implicates GATA4 as a transcriptional repressor of fibroblast growth factor 15 (Fgf15, which encodes an enterokine that has been implicated in an increasing number of human diseases. Conclusions: Overall, this study refines our understanding of an important GATA4-dependent molecular mechanism to pattern the intestinal epithelium along its cephalocaudal axis by elaborating on GATA4’s function as a crucial dominant molecular determinant of jejunal enterocyte identity. Microarray data from this study have been deposited into

  16. cAMP-dependent proteolysis of GATA-6 is linked to JNK-signaling pathway

    Energy Technology Data Exchange (ETDEWEB)

    Ushijima, Hironori [Department of Molecular Biology, School of Pharmacy, Iwate Medical University, 2-1-1, Nishitokuta, Yahaba, Shiwagun, Iwate 028-3694 (Japan); Maeda, Masatomo, E-mail: mmaeda@iwate-med.ac.jp [Department of Molecular Biology, School of Pharmacy, Iwate Medical University, 2-1-1, Nishitokuta, Yahaba, Shiwagun, Iwate 028-3694 (Japan)

    2012-07-13

    Highlights: Black-Right-Pointing-Pointer A JNK inhibitor SP600125 inhibited cAMP-dependent proteolysis of GATA-6. Black-Right-Pointing-Pointer Effect of a JNK activator anisomycin on the proteolysis was examined. Black-Right-Pointing-Pointer Anisomycin stimulated the export of nuclear GATA-6 into the cytoplasm. Black-Right-Pointing-Pointer JNK activated the CRM1 mediated nuclear export of GATA-6. Black-Right-Pointing-Pointer JNK further stimulated slowly the degradation of GATA-6 by cytoplasmic proteasomes. -- Abstract: A JNK inhibitor SP600125 inhibited cAMP-dependent proteolysis of GATA-6 by proteasomes around its IC50. We further examined the effects of SP600125 on the degradation of GATA-6 in detail, since an activator of JNK (anisomycin) is available. Interestingly, anisomycin immediately stimulated the export of nuclear GATA-6 into the cytoplasm, and then the cytoplasmic content of GATA-6 decreased slowly through degradation by proteasomes. Such an effect of anisomycin was inhibited by SP600125, indicating that the observed phenomenon might be linked to the JNK signaling pathway. The inhibitory effect of SP600125 could not be ascribed to the inhibition of PKA, since phosphorylation of CREB occurred in the presence of dbcAMP and SP600125. The nuclear export of GATA-6 was inhibited by leptomycin B, suggesting that CRM1-mediated export could be activated by anisomycin. Furthermore, it seems likely that the JNK activated by anisomycin may stimulate not only the nuclear export of GATA-6 through CRM1 but also the degradation of GATA-6 by cytoplasmic proteasomes. In contrast, A-kinase might activate only the latter process through JNK.

  17. Transcription factor GATA-4 is a marker of anaplasia in adrenocortical neoplasms of the domestic ferret (Mustela putorius furo).

    Science.gov (United States)

    Peterson, R A; Kiupel, M; Bielinska, M; Kiiveri, S; Heikinheimo, M; Capen, C C; Wilson, D B

    2004-07-01

    Adrenocortical neoplasms are a common cause of morbidity in neutered ferrets. Recently we showed that gonadectomized DBA/2J mice develop adrenocortical tumors that express transcription factor GATA-4. Therefore, we screened archival specimens of adrenocortical neoplasms from neutered ferrets to determine whether GATA-4 could be used as a tumor marker in this species. Nuclear immunoreactivity for GATA-4 was evident in 19/22 (86%) of ferret adrenocortical carcinomas and was prominent in areas exhibiting myxoid differentiation. Normal adrenocortical cells lacked GATA-4 expression. Two other markers of adrenocortical tumors in gonadectomized mice, inhibin-alpha and luteinizing hormone receptor, were coexpressed with GATA-4 in some of the ferret tumors. No GATA-4 expression was observed in three cases of nodular hyperplasia, but patches of anaplastic cells expressing GATA-4 were evident in 7/14 (50%) of tumors classified as adenomas. We conclude that GATA-4 can function as a marker of anaplasia in ferret adrenocortical tumors.

  18. Estrogen promotes megakaryocyte polyploidization via estrogen receptor beta-mediated transcription of GATA1.

    Science.gov (United States)

    Du, C; Xu, Y; Yang, K; Chen, S; Wang, X; Wang, S; Wang, C; Shen, M; Chen, F; Chen, M; Zeng, D; Li, F; Wang, T; Wang, F; Zhao, J; Ai, G; Cheng, T; Su, Y; Wang, J

    2017-04-01

    Estrogen is reported to be involved in thrombopoiesis and the disruption of its signaling may cause myeloproliferative disease, yet the underlying mechanisms remain largely unknown. GATA-binding factor 1 (GATA1) is a key regulator of megakaryocyte (MK) differentiation and its deficiency will lead to megakaryoblastic leukemia. Here we show that estrogen can dose-dependently promote MK polyploidization and maturation via activation of estrogen receptor beta (ERβ), accompanied by a significant upregulation of GATA1. Chromatin immunoprecipitation and a dual luciferase assay demonstrate that ERβ can directly bind the promoter region of GATA1 and activate its transcription. Steroid receptor coactivator 3 (SRC3) is involved in ERβ-mediated GATA1 transcription. The deficiency of ERβ or SRC3, similar to the inhibition of GATA1, leads to the impediment of estrogen-induced MK polyploidization and platelet production. Further investigations reveal that signal transducer and activator of transcription 1 signaling pathway downstream of GATA1 has a crucial role in estrogen-induced MK polyploidization, and ERβ-mediated GATA1 upregulation subsequently enhances nuclear factor erythroid-derived 2 expression, thereby promoting proplatelet formation and platelet release. Our study provides a deep insight into the molecular mechanisms of estrogen signaling in regulating thrombopoiesis and the pathogenesis of ER deficiency-related leukemia.

  19. The Gata3 transcription factor is required for the survival of embryonic and adult sympathetic neurons

    NARCIS (Netherlands)

    K. Tsarovina (Konstantina); T. Reiff (Tobias); J. Stubbusch (Jutta); D. Kurek (Dorota); F.G. Grosveld (Frank); R. Parlato (Rosanna); G. Schütz (Günther); H. Rohrer (Hermann)

    2010-01-01

    textabstractThe transcription factor Gata3 is essential for the development of sympathetic neurons and adrenal chromaffin cells. As Gata3 expression is maintained up to the adult stage, we addressed its function in differentiated sympathoadrenal cells at embryonic and adult stages by conditional

  20. GATA-3 is involved in the development of serotonergic neurons in the caudal raphe nuclei

    NARCIS (Netherlands)

    J. van der Wees (Jacqueline); A. Karis (Alar); E. Goedknegt; M. Rutteman; F.G. Grosveld (Frank); J.H. van Doorninck (Hikke); C.I. de Zeeuw (Chris)

    1999-01-01

    textabstractAbstract The GATA-3 transcription factor shows a specific and restricted expression pattern in the developing and adult mouse brain. In the present study we investigated the role of GATA-3 in the caudal raphe system, which is known to operate as a modulator of motor activity. We

  1. Molecular analysis of the interaction between the hematopoietic master transcription factors GATA-1 and PU.1

    DEFF Research Database (Denmark)

    Liew, Chu Wai; Rand, Kasper Dyrberg; Simpson, Raina J Y

    2006-01-01

    GATA-1 and PU.1 are transcription factors that control erythroid and myeloid development, respectively. The two proteins have been shown to function in an antagonistic fashion, with GATA-1 repressing PU.1 activity during erythropoiesis and PU.1 repressing GATA-1 function during myelopoiesis. It has...... also become clear that this functional antagonism involves direct interactions between the two proteins. However, the molecular basis for these interactions is not known, and a number of inconsistencies exist in the literature. We have used a range of biophysical methods to define the molecular details...... of the GATA-1-PU.1 interaction. A combination of NMR titration data and extensive mutagenesis revealed that the PU.1-Ets domain and the GATA-1 C-terminal zinc finger (CF) form a low affinity interaction in which specific regions of each protein are implicated. Surprisingly, the interaction cannot be disrupted...

  2. A Gata2-Dependent Transcription Network Regulates Uterine Progesterone Responsiveness and Endometrial Function

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    Cory A. Rubel

    2016-10-01

    Full Text Available Altered progesterone responsiveness leads to female infertility and cancer, but underlying mechanisms remain unclear. Mice with uterine-specific ablation of GATA binding protein 2 (Gata2 are infertile, showing failures in embryo implantation, endometrial decidualization, and uninhibited estrogen signaling. Gata2 deficiency results in reduced progesterone receptor (PGR expression and attenuated progesterone signaling, as evidenced by genome-wide expression profiling and chromatin immunoprecipitation. GATA2 not only occupies at and promotes expression of the Pgr gene but also regulates downstream progesterone responsive genes in conjunction with the PGR. Additionally, Gata2 knockout uteri exhibit abnormal luminal epithelia with ectopic TRP63 expressing squamous cells and a cancer-related molecular profile in a progesterone-independent manner. Lastly, we found a conserved GATA2-PGR regulatory network in both human and mice based on gene signature and path analyses using gene expression profiles of human endometrial tissues. In conclusion, uterine Gata2 regulates a key regulatory network of gene expression for progesterone signaling at the early pregnancy stage.

  3. GATA-3 EXPRESSION IN PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH BRONCHIAL ASTHMA

    Directory of Open Access Journals (Sweden)

    V. N. Mineev

    2010-01-01

    Full Text Available The aim of the study is to establish the features of expression of GATA-3 in peripheral lymphocytes from bronchial asthma patients (BA. Material and methods. 10 healthy controls, 15 patients with allergic (atopic and 15 persons with non-allergic BA were examined. A transcription factor GATA-3 expressed in peripheral lymphocytes was analyzed by Western blot after the lymphocytes were lysed. Preparation of cell lysates, and Western blotting were performed by means of a standard procedure (Amersham. An antibody against GATA-3 (Abcam, UK was used. Levels of the protein were analyzed versus β-actin levels using anti-actin antibody (Sigma Aldrich, USA. Results. Expression of GATA-3 was significantly increased in lymphocytes of patients with allergic BA as compared to healthy persons and non-allergic BA patients. The level of GATA-3 negatively correlated with the degree of airflow obstruction and positively correlated with dosage of parenteral steroids administered. Conclusion. GATA-3 may play a key role in the pathophysiology of BA. One may suggest that increased expression of GATA-3 transcription factor in atopic BA underlie high levels of Th2-cytokines production in allergic disease

  4. SUMOylation regulates the transcriptional repression activity of FOG-2 and its association with GATA-4.

    Science.gov (United States)

    Perdomo, José; Jiang, Xing-Mai; Carter, Daniel R; Khachigian, Levon M; Chong, Beng H

    2012-01-01

    Friend of GATA 2 (FOG-2), a co-factor of several GATA transcription factors (GATA-4, -5 and 6), is a critical regulator of coronary vessel formation and heart morphogenesis. Here we demonstrate that FOG-2 is SUMOylated and that this modification modulates its transcriptional activity. FOG-2 SUMOylation occurs at four lysine residues (K324, 471, 915, 955) [corrected]. Three of these residues are part of the characteristic SUMO consensus site (ψKXE), while K955 is found in the less frequent TKXE motif. Absence of SUMOylation did not affect FOG-2's nuclear localization. However, mutation of the FOG-2 SUMOylation sites, or de-SUMOylation, with SENP-1 or SENP-8 resulted in stronger transcriptional repression activity in both heterologous cells and cardiomyocytes. Conversely, increased FOG-2 SUMOylation by overexpression of SUMO-1 or expression of a SUMO-1-FOG-2 fusion protein rendered FOG-2 incapable of repressing GATA-4-mediated activation of the B-type natriuretic peptide (BNP) promoter. Moreover, we demonstrate both increased interaction between a FOG-2 SUMO mutant and GATA-4 and enhanced SUMOylation of wild-type FOG-2 by co-expression of GATA-4. These data suggest a new dynamics in which GATA-4 may alter the activity of FOG-2 by influencing its SUMOylation status.

  5. Regulation of an Autoimmune Model for Multiple Sclerosis in Th2-Biased GATA3 Transgenic Mice

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    Viromi Fernando

    2014-01-01

    Full Text Available T helper (Th2 cells have been proposed to play a neuroprotective role in multiple sclerosis (MS. This is mainly based on “loss-of-function” studies in an animal model for MS, experimental autoimmune encephalomyelitis (EAE, using blocking antibodies against Th2 related cytokines, and knockout mice lacking Th2-related molecules. We tested whether an increase of Th2 responses (“gain-of-function” approach could alter EAE, the approach of novel GATA binding protein 3 (GATA3-transgenic (tg mice that overexpress GATA3, a transcription factor required for Th2 differentiation. In EAE induced with myelin oligodendrocyte glycoprotein (MOG35−55 peptide, GATA3-tg mice had a significantly delayed onset of disease and a less severe maximum clinical score, compared with wild-type C57BL/6 mice. Histologically, GATA3-tg mice had decreased levels of meningitis and demyelination in the spinal cord, and anti-inflammatory cytokine profiles immunologically, however both groups developed similar levels of MOG-specific lymphoproliferative responses. During the early stage, we detected higher levels of interleukin (IL-4 and IL-10, with MOG and mitogen stimulation of regional lymph node cells in GATA3-tg mice. During the late stage, only mitogen stimulation induced higher IL-4 and lower interferon-γ and IL-17 production in GATA3-tg mice. These results suggest that a preexisting bias toward a Th2 immune response may reduce the severity of inflammatory demyelinating diseases, including MS.

  6. Genomic profiling identifies GATA6 as a candidate oncogene amplified in pancreatobiliary cancer.

    Directory of Open Access Journals (Sweden)

    Kevin A Kwei

    2008-05-01

    Full Text Available Pancreatobiliary cancers have among the highest mortality rates of any cancer type. Discovering the full spectrum of molecular genetic alterations may suggest new avenues for therapy. To catalogue genomic alterations, we carried out array-based genomic profiling of 31 exocrine pancreatic cancers and 6 distal bile duct cancers, expanded as xenografts to enrich the tumor cell fraction. We identified numerous focal DNA amplifications and deletions, including in 19% of pancreatobiliary cases gain at cytoband 18q11.2, a locus uncommonly amplified in other tumor types. The smallest shared amplification at 18q11.2 included GATA6, a transcriptional regulator previously linked to normal pancreas development. When amplified, GATA6 was overexpressed at both the mRNA and protein levels, and strong immunostaining was observed in 25 of 54 (46% primary pancreatic cancers compared to 0 of 33 normal pancreas specimens surveyed. GATA6 expression in xenografts was associated with specific microarray gene-expression patterns, enriched for GATA binding sites and mitochondrial oxidative phosphorylation activity. siRNA mediated knockdown of GATA6 in pancreatic cancer cell lines with amplification led to reduced cell proliferation, cell cycle progression, and colony formation. Our findings indicate that GATA6 amplification and overexpression contribute to the oncogenic phenotypes of pancreatic cancer cells, and identify GATA6 as a candidate lineage-specific oncogene in pancreatobiliary cancer, with implications for novel treatment strategies.

  7. SUMOylation regulates the transcriptional repression activity of FOG-2 and its association with GATA-4.

    Directory of Open Access Journals (Sweden)

    José Perdomo

    Full Text Available Friend of GATA 2 (FOG-2, a co-factor of several GATA transcription factors (GATA-4, -5 and 6, is a critical regulator of coronary vessel formation and heart morphogenesis. Here we demonstrate that FOG-2 is SUMOylated and that this modification modulates its transcriptional activity. FOG-2 SUMOylation occurs at four lysine residues (K324, 471, 915, 955 [corrected]. Three of these residues are part of the characteristic SUMO consensus site (ψKXE, while K955 is found in the less frequent TKXE motif. Absence of SUMOylation did not affect FOG-2's nuclear localization. However, mutation of the FOG-2 SUMOylation sites, or de-SUMOylation, with SENP-1 or SENP-8 resulted in stronger transcriptional repression activity in both heterologous cells and cardiomyocytes. Conversely, increased FOG-2 SUMOylation by overexpression of SUMO-1 or expression of a SUMO-1-FOG-2 fusion protein rendered FOG-2 incapable of repressing GATA-4-mediated activation of the B-type natriuretic peptide (BNP promoter. Moreover, we demonstrate both increased interaction between a FOG-2 SUMO mutant and GATA-4 and enhanced SUMOylation of wild-type FOG-2 by co-expression of GATA-4. These data suggest a new dynamics in which GATA-4 may alter the activity of FOG-2 by influencing its SUMOylation status.

  8. GATA4 Regulates Epithelial Cell Proliferation to Control Intestinal Growth and Development in MiceSummary

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    Bridget M. Kohlnhofer

    2016-03-01

    Full Text Available Background & Aims: The embryonic small intestinal epithelium is highly proliferative, and although much is known about mechanisms regulating proliferation in the adult intestine, the mechanisms controlling epithelial cell proliferation in the developing intestine are less clear. GATA4, a transcription factor that regulates proliferation in other developing tissues, is first expressed early in the developing gut in midgut endoderm. GATA4 function within midgut endoderm and the early intestinal epithelium is unknown. Methods: By using Sonic Hedgehog Cre to eliminate GATA4 in the midgut endoderm of mouse embryos, we determined the impact of loss of GATA4 on intestinal development, including epithelial cell proliferation, between embryonic day (E9.5 and E18.5. Results: We found that intestinal length and width were decreased in GATA4 mutants compared with controls. GATA4-deficient intestinal epithelium contained fewer cells, and epithelial girth was decreased. We further observed a decreased proportion of proliferating epithelial cells at E10.5 and E11.5 in GATA4 mutants. We showed that GATA4 binds to chromatin containing GATA4 consensus binding sites within cyclin D2 (Ccnd2, cyclin-dependent kinase 6 (Cdk6, and frizzled 5 (Fzd5. Moreover, Ccnd2, Cdk6, and Fzd5 transcripts were reduced at E11.5 in GATA4 mutant tissue. Villus morphogenesis was delayed, and villus structure was abnormal in GATA4 mutant intestine. Conclusions: Our data identify GATA4 as an essential regulator of early intestinal epithelial cell proliferation. We propose that GATA4 controls proliferation in part by directly regulating transcription of cell-cycle mediators. Our data further suggest that GATA4 affects proliferation through transcriptional regulation of Fzd5, perhaps by influencing the response of the epithelium to WNT signaling. Keywords: Transcriptional Regulation, WNT Signaling, Villus Morphogenesis

  9. Hypoxia-inducible factor 1-mediated human GATA1 induction promotes erythroid differentiation under hypoxic conditions.

    Science.gov (United States)

    Zhang, Feng-Lin; Shen, Guo-Min; Liu, Xiao-Ling; Wang, Fang; Zhao, Ying-Ze; Zhang, Jun-Wu

    2012-08-01

    Hypoxia-inducible factor promotes erythropoiesis through coordinated cell type-specific hypoxia responses. GATA1 is essential to normal erythropoiesis and plays a crucial role in erythroid differentiation. In this study, we show that hypoxia-induced GATA1 expression is mediated by HIF1 in erythroid cells. Under hypoxic conditions, significantly increased GATA1 mRNA and protein levels were detected in K562 cells and erythroid induction cultures of CD34(+) haematopoietic stem/progenitor cells. Enforced HIF1α expression increased GATA1 expression, while HIF1α knockdown by RNA interference decreased GATA1 expression. In silico analysis revealed one potential hypoxia response element (HRE). The results from reporter gene and mutation analysis suggested that this element is necessary for hypoxic response. Chromatin immunoprecipitation (ChIP)-PCR showed that the putative HRE was recognized and bound by HIF1 in vivo. These results demonstrate that the up-regulation of GATA1 during hypoxia is directly mediated by HIF1.The mRNA expression of some erythroid differentiation markers was increased under hypoxic conditions, but decreased with RNA interference of HIF1α or GATA1. Flow cytometry analysis also indicated that hypoxia, desferrioxamine or CoCl(2) induced expression of erythroid surface markers CD71 and CD235a, while expression repression of HIF1α or GATA1 by RNA interference led to a decreased expression of CD235a. These results suggested that HIF1-mediated GATA1 up-regulation promotes erythropoiesis in order to satisfy the needs of an organism under hypoxic conditions. © 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.

  10. Identification of functional mutations in GATA4 in patients with congenital heart disease.

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    Erli Wang

    Full Text Available Congenital heart disease (CHD is one of the most prevalent developmental anomalies and the leading cause of noninfectious morbidity and mortality in newborns. Despite its prevalence and clinical significance, the etiology of CHD remains largely unknown. GATA4 is a highly conserved transcription factor that regulates a variety of physiological processes and has been extensively studied, particularly on its role in heart development. With the combination of TBX5 and MEF2C, GATA4 can reprogram postnatal fibroblasts into functional cardiomyocytes directly. In the past decade, a variety of GATA4 mutations were identified and these findings originally came from familial CHD pedigree studies. Given that familial and sporadic CHD cases allegedly share a basic genetic basis, we explore the GATA4 mutations in different types of CHD. In this study, via direct sequencing of the GATA4 coding region and exon-intron boundaries in 384 sporadic Chinese CHD patients, we identified 12 heterozygous non-synonymous mutations, among which 8 mutations were only found in CHD patients when compared with 957 controls. Six of these non-synonymous mutations have not been previously reported. Subsequent functional analyses revealed that the transcriptional activity, subcellular localization and DNA binding affinity of some mutant GATA4 proteins were significantly altered. Our results expand the spectrum of GATA4 mutations linked to cardiac defects. Together with the newly reported mutations, approximately 110 non-synonymous mutations have currently been identified in GATA4. Our future analysis will explore why the evolutionarily conserved GATA4 appears to be hypermutable.

  11. Gene expression profiling identifies HOXB4 as a direct downstream target of GATA-2 in human CD34+ hematopoietic cells.

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    Tohru Fujiwara

    Full Text Available Aplastic anemia is characterized by a reduced hematopoietic stem cell number. Although GATA-2 expression was reported to be decreased in CD34-positive cells in aplastic anemia, many questions remain regarding the intrinsic characteristics of hematopoietic stem cells in this disease. In this study, we identified HOXB4 as a downstream target of GATA-2 based on expression profiling with human cord blood-derived CD34-positive cells infected with control or GATA-2 lentiviral shRNA. To confirm the functional link between GATA-2 and HOXB4, we conducted GATA-2 gain-of-function and loss-of-function experiments, and HOXB4 promoter analysis, including luciferase assay, in vitro DNA binding analysis and quantitative ChIP analysis, using K562 and CD34-positive cells. The analyses suggested that GATA-2 directly regulates HOXB4 expression through the GATA sequence in the promoter region. Furthermore, we assessed GATA-2 and HOXB4 expression in CD34-positive cells from patients with aplastic anemia (n = 10 and idiopathic thrombocytopenic purpura (n = 13, and demonstrated that the expression levels of HOXB4 and GATA-2 were correlated in these populations (r = 0.6573, p<0.01. Our results suggested that GATA-2 directly regulates HOXB4 expression in hematopoietic stem cells, which may play an important role in the development and/or progression of aplastic anemia.

  12. Electrostatic study of Alanine mutational effects on transcription: application to GATA-3:DNA interaction complex.

    Science.gov (United States)

    El-Assaad, Atlal; Dawy, Zaher; Nemer, Georges

    2015-01-01

    Protein-DNA interaction is of fundamental importance in molecular biology, playing roles in functions as diverse as DNA transcription, DNA structure formation, and DNA repair. Protein-DNA association is also important in medicine; understanding Protein-DNA binding kinetics can assist in identifying disease root causes which can contribute to drug development. In this perspective, this work focuses on the transcription process by the GATA Transcription Factor (TF). GATA TF binds to DNA promoter region represented by `G,A,T,A' nucleotides sequence, and initiates transcription of target genes. When proper regulation fails due to some mutations on the GATA TF protein sequence or on the DNA promoter sequence (weak promoter), deregulation of the target genes might lead to various disorders. In this study, we aim to understand the electrostatic mechanism behind GATA TF and DNA promoter interactions, in order to predict Protein-DNA binding in the presence of mutations, while elaborating on non-covalent binding kinetics. To generate a family of mutants for the GATA:DNA complex, we replaced every charged amino acid, one at a time, with a neutral amino acid like Alanine (Ala). We then applied Poisson-Boltzmann electrostatic calculations feeding into free energy calculations, for each mutation. These calculations delineate the contribution to binding from each Ala-replaced amino acid in the GATA:DNA interaction. After analyzing the obtained data in view of a two-step model, we are able to identify potential key amino acids in binding. Finally, we applied the model to GATA-3:DNA (crystal structure with PDB-ID: 3DFV) binding complex and validated it against experimental results from the literature.

  13. Anisomycin-induced GATA-6 degradation accompanying a decrease of proliferation of colorectal cancer cell

    Energy Technology Data Exchange (ETDEWEB)

    Ushijima, Hironori; Horyozaki, Akiko; Maeda, Masatomo, E-mail: mmaeda@nupals.ac.jp

    2016-09-09

    Transcription factor GATA-6 plays a key role in normal cell differentiation of the mesoderm and endoderm. On the other hand, GATA-6 is abnormally overexpressed in many clinical gastrointestinal cancer tissue samples, and accelerates cell proliferation or an anti-apoptotic response in cancerous tissues. We previously showed that activation of the JNK signaling cascade causes proteolysis of GATA-6. In this study, we demonstrated that anisomycin, a JNK activator, stimulates nuclear export of GATA-6 in a colorectal cancer cell line, DLD-1. Concomitantly, anisomycin remarkably inhibits the proliferation of DLD-1 cells via G2/M arrest in a plate culture. However, it did not induce apoptosis under growth arrest conditions. Furthermore, the growth of DLD-1 cells in a spheroid culture was suppressed by anisomycin. Although 5-FU showed only a slight inhibitory effect on 3D spheroid cultures, the same concentration of 5-FU together with a low concentration of anisomycin exhibited strong growth inhibition. These results suggest that the induction of GATA-6 dysfunction may be more effective for chemotherapy for colorectal cancer, although the mechanism underlying the synergistic effect of 5-FU and anisomycin remains unknown. - Highlights: • Anisomycin induces proteolysis of GATA-6 in DLD-1 cells. • Anisomycin remarkably inhibits the proliferation of DLD-1 cells via G2/M arrest. • Anisomycin suppresses the growth of spheroids of DLD-1, and enhances the effect of 5-FU.

  14. Mechanism governing heme synthesis reveals a GATA factor/heme circuit that controls differentiation.

    Science.gov (United States)

    Tanimura, Nobuyuki; Miller, Eli; Igarashi, Kazuhiko; Yang, David; Burstyn, Judith N; Dewey, Colin N; Bresnick, Emery H

    2016-02-01

    Metal ion-containing macromolecules have fundamental roles in essentially all biological processes throughout the evolutionary tree. For example, iron-containing heme is a cofactor in enzyme catalysis and electron transfer and an essential hemoglobin constituent. To meet the intense demand for hemoglobin assembly in red blood cells, the cell type-specific factor GATA-1 activates transcription of Alas2, encoding the rate-limiting enzyme in heme biosynthesis, 5-aminolevulinic acid synthase-2 (ALAS-2). Using genetic editing to unravel mechanisms governing heme biosynthesis, we discovered a GATA factor- and heme-dependent circuit that establishes the erythroid cell transcriptome. CRISPR/Cas9-mediated ablation of two Alas2 intronic cis elements strongly reduces GATA-1-induced Alas2 transcription, heme biosynthesis, and surprisingly, GATA-1 regulation of other vital constituents of the erythroid cell transcriptome. Bypassing ALAS-2 function in Alas2 cis element-mutant cells by providing its catalytic product 5-aminolevulinic acid rescues heme biosynthesis and the GATA-1-dependent genetic network. Heme amplifies GATA-1 function by downregulating the heme-sensing transcriptional repressor Bach1 and via a Bach1-insensitive mechanism. Through this dual mechanism, heme and a master regulator collaborate to orchestrate a cell type-specific transcriptional program that promotes cellular differentiation. © 2015 The Authors.

  15. Efficient production of platelets from mouse embryonic stem cells by enforced expression of Gata2 in late hemogenic endothelial cells

    Energy Technology Data Exchange (ETDEWEB)

    Kawaguchi, Manami [Stem Cell Project, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506 (Japan); Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510 (Japan); Kitajima, Kenji [Stem Cell Project, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506 (Japan); Kanokoda, Mai [Stem Cell Project, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506 (Japan); Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510 (Japan); Suzuki, Hidenori [Division of Morphological and Biomolecular Research, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8602 (Japan); Miyashita, Kazuya; Nakajima, Marino [Stem Cell Project, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506 (Japan); Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510 (Japan); Nuriya, Hideko [Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506 (Japan); Kasahara, Kohji [Laboratory of Biomembrane, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506 (Japan); Hara, Takahiko, E-mail: hara-tk@igakuken.or.jp [Stem Cell Project, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo, 156-8506 (Japan); Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510 (Japan)

    2016-06-03

    Platelets are essential for blood circulation and coagulation. Previous study indicated that overexpression of Gata2 in differentiated mouse embryonic stem cells (ESCs) resulted in robust induction of megakaryocytes (Mks). To evaluate platelet production capacity of the Gata2-induced ESC-derived Mks, we generated iGata2-ESC line carrying the doxycycline-inducible Gata2 expression cassette. When doxycycline was added to day 5 hemogenic endothelial cells in the in vitro differentiation culture of iGata2-ESCs, c-Kit{sup −}Tie2{sup −}CD41{sup +} Mks were predominantly generated. These iGata2-ESC-derived Mks efficiently produced CD41{sup +}CD42b{sup +}CD61{sup +} platelets and adhered to fibrinogen-coated glass coverslips in response to thrombin stimulation. Transmission electron microscopy analysis demonstrated that the iGata2-ESC-derived platelets were discoid-shaped with α-granules and an open canalicular system, but were larger than peripheral blood platelets in size. These results demonstrated that an enforced expression of Gata2 in late HECs of differentiated ESCs efficiently promotes megakaryopoiesis followed by platelet production. This study provides valuable information for ex vivo platelet production from human pluripotent stem cells in future. -- Highlights: •Megakaryocytes are efficiently induced by Gata2 from ESC-derived day 5 HECs. •Gata2-induced ESC-derived megakaryocytes are c-Kit{sup −}Tie2{sup −}CD41{sup +}. •Gata2-induced ESC-derived megakaryocytes produce larger discoid-shaped platelets. •Gata2-induced ESC-derived platelets bind fibrinogen upon thrombin stimulation.

  16. Efficient production of platelets from mouse embryonic stem cells by enforced expression of Gata2 in late hemogenic endothelial cells

    International Nuclear Information System (INIS)

    Kawaguchi, Manami; Kitajima, Kenji; Kanokoda, Mai; Suzuki, Hidenori; Miyashita, Kazuya; Nakajima, Marino; Nuriya, Hideko; Kasahara, Kohji; Hara, Takahiko

    2016-01-01

    Platelets are essential for blood circulation and coagulation. Previous study indicated that overexpression of Gata2 in differentiated mouse embryonic stem cells (ESCs) resulted in robust induction of megakaryocytes (Mks). To evaluate platelet production capacity of the Gata2-induced ESC-derived Mks, we generated iGata2-ESC line carrying the doxycycline-inducible Gata2 expression cassette. When doxycycline was added to day 5 hemogenic endothelial cells in the in vitro differentiation culture of iGata2-ESCs, c-Kit − Tie2 − CD41 + Mks were predominantly generated. These iGata2-ESC-derived Mks efficiently produced CD41 + CD42b + CD61 + platelets and adhered to fibrinogen-coated glass coverslips in response to thrombin stimulation. Transmission electron microscopy analysis demonstrated that the iGata2-ESC-derived platelets were discoid-shaped with α-granules and an open canalicular system, but were larger than peripheral blood platelets in size. These results demonstrated that an enforced expression of Gata2 in late HECs of differentiated ESCs efficiently promotes megakaryopoiesis followed by platelet production. This study provides valuable information for ex vivo platelet production from human pluripotent stem cells in future. -- Highlights: •Megakaryocytes are efficiently induced by Gata2 from ESC-derived day 5 HECs. •Gata2-induced ESC-derived megakaryocytes are c-Kit − Tie2 − CD41 + . •Gata2-induced ESC-derived megakaryocytes produce larger discoid-shaped platelets. •Gata2-induced ESC-derived platelets bind fibrinogen upon thrombin stimulation.

  17. Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2.

    Directory of Open Access Journals (Sweden)

    Jeffrey R Shearstone

    Full Text Available Therapeutic intervention aimed at reactivation of fetal hemoglobin protein (HbF is a promising approach for ameliorating sickle cell disease (SCD and β-thalassemia. Previous studies showed genetic knockdown of histone deacetylase (HDAC 1 or 2 is sufficient to induce HbF. Here we show that ACY-957, a selective chemical inhibitor of HDAC1 and 2 (HDAC1/2, elicits a dose and time dependent induction of γ-globin mRNA (HBG and HbF in cultured primary cells derived from healthy individuals and sickle cell patients. Gene expression profiling of erythroid progenitors treated with ACY-957 identified global changes in gene expression that were significantly enriched in genes previously shown to be affected by HDAC1 or 2 knockdown. These genes included GATA2, which was induced greater than 3-fold. Lentiviral overexpression of GATA2 in primary erythroid progenitors increased HBG, and reduced adult β-globin mRNA (HBB. Furthermore, knockdown of GATA2 attenuated HBG induction by ACY-957. Chromatin immunoprecipitation and sequencing (ChIP-Seq of primary erythroid progenitors demonstrated that HDAC1 and 2 occupancy was highly correlated throughout the GATA2 locus and that HDAC1/2 inhibition led to elevated histone acetylation at well-known GATA2 autoregulatory regions. The GATA2 protein itself also showed increased binding at these regions in response to ACY-957 treatment. These data show that chemical inhibition of HDAC1/2 induces HBG and suggest that this effect is mediated, at least in part, by histone acetylation-induced activation of the GATA2 gene.

  18. GATA-1 and c-myb crosstalk during red blood cell differentiation through GATA-1 binding sites in the c-myb promoter

    Czech Academy of Sciences Publication Activity Database

    Bartůněk, Petr; Králová, Jarmila; Blendiger, G.; Dvořák, Michal; Zenke, M.

    2003-01-01

    Roč. 22, č. 13 (2003), s. 1927-1935 ISSN 0950-9232 R&D Projects: GA ČR GV301/98/K042 Institutional research plan: CEZ:AV0Z5052915 Keywords : GATA-1 * c-myb * erythropoiesis Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 6.495, year: 2003

  19. A single cis element maintains repression of the key developmental regulator Gata2.

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    Jonathan W Snow

    2010-09-01

    Full Text Available In development, lineage-restricted transcription factors simultaneously promote differentiation while repressing alternative fates. Molecular dissection of this process has been challenging as transcription factor loci are regulated by many trans-acting factors functioning through dispersed cis elements. It is not understood whether these elements function collectively to confer transcriptional regulation, or individually to control specific aspects of activation or repression, such as initiation versus maintenance. Here, we have analyzed cis element regulation of the critical hematopoietic factor Gata2, which is expressed in early precursors and repressed as GATA-1 levels rise during terminal differentiation. We engineered mice lacking a single cis element -1.8 kb upstream of the Gata2 transcriptional start site. Although Gata2 is normally repressed in late-stage erythroblasts, the -1.8 kb mutation unexpectedly resulted in reactivated Gata2 transcription, blocked differentiation, and an aberrant lineage-specific gene expression pattern. Our findings demonstrate that the -1.8 kb site selectively maintains repression, confers a specific histone modification pattern and expels RNA Polymerase II from the locus. These studies reveal how an individual cis element establishes a normal developmental program via regulating specific steps in the mechanism by which a critical transcription factor is repressed.

  20. GATA2 is associated with familial early-onset coronary artery disease.

    Directory of Open Access Journals (Sweden)

    Jessica J Connelly

    2006-08-01

    Full Text Available The transcription factor GATA2 plays an essential role in the establishment and maintenance of adult hematopoiesis. It is expressed in hematopoietic stem cells, as well as the cells that make up the aortic vasculature, namely aortic endothelial cells and smooth muscle cells. We have shown that GATA2 expression is predictive of location within the thoracic aorta; location is suggested to be a surrogate for disease susceptibility. The GATA2 gene maps beneath the Chromosome 3q linkage peak from our family-based sample set (GENECARD study of early-onset coronary artery disease. Given these observations, we investigated the relationship of several known and novel polymorphisms within GATA2 to coronary artery disease. We identified five single nucleotide polymorphisms that were significantly associated with early-onset coronary artery disease in GENECARD. These results were validated by identifying significant association of two of these single nucleotide polymorphisms in an independent case-control sample set that was phenotypically similar to the GENECARD families. These observations identify GATA2 as a novel susceptibility gene for coronary artery disease and suggest that the study of this transcription factor and its downstream targets may uncover a regulatory network important for coronary artery disease inheritance.

  1. Inhibition of lectin-like oxidized low-density lipoprotein receptor-1 reduces cardiac fibroblast proliferation by suppressing GATA Binding Protein 4

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Bin; Liu, Ning-Ning; Liu, Wei-Hua; Zhang, Shuang-Wei; Zhang, Jing-Zhi; Li, Ai-Qun [Department of Cardiology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou (China); Guangzhou Institute of Cardiovascular Disease, Guangzhou (China); Liu, Shi-Ming, E-mail: gzliushiming@126.com [Department of Cardiology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou (China); Guangzhou Institute of Cardiovascular Disease, Guangzhou (China)

    2016-07-08

    Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) and GATA Binding Protein 4 (GATA4) are important for the growth of cardiac fibroblasts (CFs). When deregulated, LOX-1 and GATA4 can cause cardiac remodeling. In the present study, we found novel evidence that GATA4 was required for the LOX-1 regulation of CF proliferation. The inhibition of LOX-1 by RNA interference LOX-1 lentivirus resulted in the loss of PI3K/Akt activation and GATA4 protein expression. The overexpression of LOX-1 by lentivirus rescued CF proliferation, PI3K/Akt activation, and GATA4 protein expression. Moreover, GATA4 overexpression enhanced CF proliferation with LOX-1 inhibition. We also found that the inhibition of PI3K/Akt activation by LY294002, a PI3K inhibitor, reduced cell proliferation and protein level of GATA4. In summary, GATA4 may play an important role in the LOX-1 and PI3K/Akt regulation of CF proliferation. -- Highlights: •GATA4 is regulated by LOX-1 signaling in CFs. •GATA4 is involved in LOX-1 regulating CF proliferation. •GATA4 is regulated by PI3K/Akt signaling in CFs.

  2. The GATA3 gene is involved in leprosy susceptibility in Brazilian patients.

    Science.gov (United States)

    Medeiros, Priscila; da Silva, Weber Laurentino; de Oliveira Gimenez, Bruna Beatriz; Vallezi, Keren Bastos; Moraes, Milton Ozório; de Souza, Vânia Niéto Brito; Latini, Ana Carla Pereira

    2016-04-01

    Leprosy outcome is a complex trait and the host-pathogen-environment interaction defines the emergence of the disease. Host genetic risk factors have been successfully associated to leprosy. The 10p13 chromosomal region was linked to leprosy in familial studies and GATA3 gene is a strong candidate to be part of this association. Here, we tested tag single nucleotide polymorphisms at GATA3 in two case-control samples from Brazil comprising a total of 1633 individuals using stepwise strategy. The A allele of rs10905284 marker was associated with leprosy resistance. Then, a functional analysis was conducted and showed that individuals carrying AA genotype express higher levels of GATA-3 protein in lymphocytes. So, we confirmed that the rs10905284 is a locus associated to leprosy and influences the levels of this transcription factor in the Brazilian population. Copyright © 2016 Elsevier B.V. All rights reserved.

  3. [Study of allelic polymorphism of (GATA)n-containing loci in parthenogenetic lizards Darevskia unisexualis (Lacertidae)].

    Science.gov (United States)

    Korchagin, V I; Martirosian, I A; Omel'chenko, A V; Darevskiĭ, I S; Ryskov, A P; Tokarskaia, O N

    2004-10-01

    The genesis of mini- and microsatellite loci, which is under extensive study in humans and some other bisexual species, have been virtually overlooked in species with clonal mode of reproduction. Earlier, using multilocus DNA fingerprinting, we have examined variability of some mini- and microsatellite DNA markers in parthenogenetic lizards from the genus Darevskia. In particular, mutant (GATA)n-restrictive DNA fragments were found in Darevskia unisexualis. In the present study, we examined intraspecific polymorphism of three cloned loci of D. unisexualis--Du323, Du215, and Du281--containing (GATA)7GAT(GATA)2, GAT(GATA)9, and (GATA)10TA(GATA) microsatellite clusters, respectively. Different levels of intrapopulation and interpopulation variability of these loci were found. Locus Du281 showed the highest polymorphism--six allelic variants (in the sample of 68 DNA specimens). Three alleles were found for locus Du215. The Du325 locus was electrophoretically invariant. The primers chosen for loci Du323, Du215, and Du281 were also used for PCR analysis of homologous loci in two presumptive parental bisexual species, D. valentini and D. nairensis. The PCR products of the corresponding loci of the parental species had approximately the same size (approximately 200 bp) as their counterparts in D. unisexualis, but the polymorphism levels of the paternal, maternal, and hybrid species were shown to be somewhat different. These data on the structure of the D. unisexualis loci provide a possibility to study genetic diversity in the parthenogenetic species D. unisexualis and other related unisexual and bisexual species of this genus, which can provide new information on the origin of parthenogenetic species and on the phylogenetic relationships in the genus Darevskia. These data can also be used for resolving problems of marking the lizard genome, which is still poorly studied.

  4. GATA3 is a master regulator of the transcriptional response to low-dose ionizing radiation in human keratinocytes

    Energy Technology Data Exchange (ETDEWEB)

    Bonin, F.; Molina, M.; Berthier-Vergnes, O.; Lamartine, J. [Universite de Lyon, Lyon, F-69003 (France); Universite Lyon 1, Lyon, F-69003 (France); CNRS, UMR5534, Centre de Genetique Moleculaire et Cellulaire, Villeurbanne, F-69622 (France); Malet, C.; Ginestet, C. [Centre Leon Berard, Service de Radiotherapie, Lyon F-69008 (France); Martin, M.T. [Laboratoire de Genomique et Radiobiologie de la Keratinopoiese, CEA, IRCM, Evry F-91000 (France)

    2009-07-01

    Background: The general population is constantly exposed to low levels of radiation through natural, occupational or medical irradiation. Even if the biological effects of low-level radiation have been intensely debated and investigated, the molecular mechanisms underlying the cellular response to low doses remain largely unknown. Results: The present study investigated the role of GATA3 protein in the control of the cellular and molecular response of human keratinocytes exposed to a 1 cGy dose of X-rays. Chromatin immunoprecipitation showed GATA3 to be able to bind the promoter of 4 genes responding to a 1 cGy exposure. To go further into the role of GATA3 after ionizing radiation exposure, we studied the cellular and molecular consequences of radiation in GATA3 knock-down cells. Knockdown was obtained by lentiviral-mediated expression of an shRNA targeting the GATA3 transcript in differentiated keratinocytes. First, radiosensitivity was assessed: the toxicity, in terms of immediate survival (with XTT test), associated with 1 cGy radiation was found to be increased in GATA3 knock-down cells. The impact of GATA3 knock-down on the transcriptome of X-ray irradiated cells was also investigated, using oligonucleotide micro-arrays to assess changes between 3 h and 72 h post-irradiation in normal vs GATA3 knock-down backgrounds; transcriptome response was found to be completely altered in GATA3 knock-down cells, with a strong induction/repression peak 48 h after irradiation. Functional annotation revealed enrichment in genes known to be involved in chaperone activity, TGF{beta} signalling and stress response. Conclusion: Taken together, these data indicate that GATA3 is an important regulator of the cellular and molecular response of epidermal cells to very low doses of radiation. (authors)

  5. Esrrb directly binds to Gata6 promoter and regulates its expression with Dax1 and Ncoa3

    International Nuclear Information System (INIS)

    Uranishi, Kousuke; Akagi, Tadayuki; Koide, Hiroshi; Yokota, Takashi

    2016-01-01

    Estrogen-related receptor beta (Esrrb) is expressed in embryonic stem (ES) cells and is involved in self-renewal ability and pluripotency. Previously, we found that Dax1 is associated with Esrrb and represses its transcriptional activity. Further, the disruption of the Dax1–Esrrb interaction increases the expression of the extra-embryonic endoderm marker Gata6 in ES cells. Here, we investigated the influences of Esrrb and Dax1 on Gata6 expression. Esrrb overexpression in ES cells induced endogenous Gata6 mRNA and Gata6 promoter activity. In addition, the Gata6 promoter was found to contain the Esrrb recognition motifs ERRE1 and ERRE2, and the latter was the responsive element of Esrrb. Associations between ERRE2 and Esrrb were then confirmed by biotin DNA pulldown and chromatin immunoprecipitation assays. Subsequently, we showed that Esrrb activity at the Gata6 promoter was repressed by Dax1, and although Dax1 did not bind to ERRE2, it was associated with Esrrb, which directly binds to ERRE2. In addition, the transcriptional activity of Esrrb was enhanced by nuclear receptor co-activator 3 (Ncoa3), which has recently been shown to be a binding partner of Esrrb. Finally, we showed that Dax1 was associated with Ncoa3 and repressed its transcriptional activity. Taken together, the present study indicates that the Gata6 promoter is activated by Esrrb in association with Ncoa3, and Dax1 inhibited activities of Esrrb and Ncoa3, resulting maintenance of the undifferentiated status of ES cells. - Highlights: • Esrrb induced Gata6 expression in ES cells. • Gata6 promoter activity was enhanced by Esrrb, which was repressed by Dax1. • Dax1 associated with the Gata6 promoter via Esrrb. • Dax1 associated with Ncoa3 and repressed its transcriptional activity.

  6. GATA3 is a master regulator of the transcriptional response to low-dose ionizing radiation in human keratinocytes

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    Bonin, F.; Molina, M.; Berthier-Vergnes, O.; Lamartine, J.; Malet, C.; Ginestet, C.; Martin, M.T.

    2009-01-01

    Background: The general population is constantly exposed to low levels of radiation through natural, occupational or medical irradiation. Even if the biological effects of low-level radiation have been intensely debated and investigated, the molecular mechanisms underlying the cellular response to low doses remain largely unknown. Results: The present study investigated the role of GATA3 protein in the control of the cellular and molecular response of human keratinocytes exposed to a 1 cGy dose of X-rays. Chromatin immunoprecipitation showed GATA3 to be able to bind the promoter of 4 genes responding to a 1 cGy exposure. To go further into the role of GATA3 after ionizing radiation exposure, we studied the cellular and molecular consequences of radiation in GATA3 knock-down cells. Knockdown was obtained by lentiviral-mediated expression of an shRNA targeting the GATA3 transcript in differentiated keratinocytes. First, radiosensitivity was assessed: the toxicity, in terms of immediate survival (with XTT test), associated with 1 cGy radiation was found to be increased in GATA3 knock-down cells. The impact of GATA3 knock-down on the transcriptome of X-ray irradiated cells was also investigated, using oligonucleotide micro-arrays to assess changes between 3 h and 72 h post-irradiation in normal vs GATA3 knock-down backgrounds; transcriptome response was found to be completely altered in GATA3 knock-down cells, with a strong induction/repression peak 48 h after irradiation. Functional annotation revealed enrichment in genes known to be involved in chaperone activity, TGFβ signalling and stress response. Conclusion: Taken together, these data indicate that GATA3 is an important regulator of the cellular and molecular response of epidermal cells to very low doses of radiation. (authors)

  7. ASSOCIATION BETWEEN SPECIFIC FEATURES OF GATA3 TRANSCRIPTION FACTOR EXPRESSION AND LYMPH NODE METASTASIS IN LUMINAL BREAST CANCER

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    S. V. Vtorushin

    2017-01-01

    Full Text Available Currently, the study of the markers of cell differentiation, proliferative regulators, and molecules involved in the development of drug resistance mechanisms in breast cancer is extremely important. The transcription factor GATA3 plays an essential role in the differentiation and proliferative activity of luminal breast cancer cells, being a tumor suppressor. The GATA3 positive expression is most frequently observed in invasive carcinoma of no special type. High expression of GATA3 is associated with low-grade ER-positive cancer with a favorable prognosis. Low GATA3 expression is observed in patients with high-grade and hormone receptor-negative cancer. The study of GATA3 expression is necessary for understanding the development of drug resistance mechanisms and developing approaches to overcome them as well as for determining the response to hormone therapy. Aim. The present study was undertaken to study the expression characteristics of the transcription factor GATA3 in patients with luminal breast cancer and to evaluate their relationship with the parameters of lymphogenous metastasis. Material and methods. The study included 64 patients with stage T1–4N1–3M0 invasive breast cancer. The primary tumor tissue and all removed lymph nodes were morphologically examined. The diagnosis was established according to the WHO criteria (2012. Results. Low GATA3 expression was associated with a high risk of lymph node metastases, while high GATA3 expression was associated with the absence of lymph node metastases. Heterogeneous GATA3 expression was associated with high risk of lymph node metastasis, and as a consequence, with poor prognosis. Conclusion. The relationship between the expression of GATA3 protein and lymphogenic metastasis in patients with luminal breast cancer was found.

  8. c-kitpos GATA-4 high rat cardiac stem cells foster adult cardiomyocyte survival through IGF-1 paracrine signalling.

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    Nanako Kawaguchi

    2010-12-01

    Full Text Available Resident c-kit positive (c-kitpos cardiac stem cells (CSCs could be considered the most appropriate cell type for myocardial regeneration therapies. However, much is still unknown regarding their biological properties and potential.We produced clones of high and low expressing GATA-4 CSCs from long-term bulk-cultured c-kitpos CSCs isolated from adult rat hearts. When c-kitpos GATA-4 high expressing clonal CSCs (cCSCs were co-cultured with adult rat ventricular cardiomyocytes, we observed increased survival and contractility of the cardiomyocytes, compared to cardiomyocytes cultured alone, co-cultured with fibroblasts or c-kitpos GATA-4 low expressing cCSCs. When analysed by ELISA, the concentration of IGF-1 was significantly increased in the c-kitpos GATA-4 high cCSC/cardiomyocyte co-cultures and there was a significant correlation between IGF-1 concentration and cardiomyocyte survival. We showed the activation of the IGF-1 receptor and its downstream molecular targets in cardiomyocytes co-cultured with c-kitpos GATA-4 high cCSCs but not in cardiomyocytes that were cultured alone, co-cultured with fibroblasts or c-kitpos GATA-4 low cCSCs. Addition of a blocking antibody specific to the IGF-1 receptor inhibited the survival of cardiomyocytes and prevented the activation of its signalling in cardiomyocytes in the c-kitpos GATA-4 high cCSC/cardiomyocyte co-culture system. IGF-1 supplementation or IGF-1 high conditioned medium taken from the co-culture of c-kitpos GATA-4 high cCSCs plus cardiomyocytes did extend the survival and contractility of cardiomyocytes cultured alone and cardiomyocytes co-cultured with c-kitpos GATA-4 low cCSCs.c-kitpos GATA-4 high cCSCs exert a paracrine survival effect on cardiomyocytes through induction of the IGF-1R and signalling pathway.

  9. Displasia cística mamária em uma gata

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    Cartagna Lúcia Maria

    2005-01-01

    Full Text Available Displasia cística mamária ou mastose é uma rara condição que afeta gatas adultas ou idosas. O presente relato descreve um caso de displasia cística mamária diagnosticado em uma gata sem raça definida de sete anos de idade, com histórico clínico de aumento de volume mamário generalizado por mais de um ano. O diagnóstico foi realizado com base nos achados clínicos, citopatológicos e histopatológicos.

  10. Statins activate GATA-6 and induce differentiated vascular smooth muscle cells

    International Nuclear Information System (INIS)

    Wada, Hiromichi; Abe, Mitsuru; Ono, Koh; Morimoto, Tatsuya; Kawamura, Teruhisa; Takaya, Tomohide; Satoh, Noriko; Fujita, Masatoshi; Kita, Toru; Shimatsu, Akira; Hasegawa, Koji

    2008-01-01

    The beneficial effects of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins) beyond cholesterol lowering involve their direct actions on vascular smooth muscle cells (VSMCs). However, the effects of statins on phenotypic modulation of VSMCs are unknown. We herein show that simvastatin (Sm) and atorvastatin (At) inhibited DNA synthesis in human aortic VSMCs dose-dependently, while cell toxicity was not observed below the concentration of 1 μM of Sm or 100 nM of At. Stimulating proliferative VSMCs with Sm or At induced the expression of SM-α-actin and SM-MHC, highly specific markers of differentiated phenotype. Sm up-regulated the binding activity of GATA-6 to SM-MHC GATA site and activated the transfected SM-MHC promoter in proliferative VSMCs, while mutating the GATA-6 binding site abolished this activation. Geranylgeranylpyrophosphate (10 μM), an inhibitor of Rho family proteins, abolished the statin-mediated induction of the differentiated phenotype in VSMCs. These findings suggest that statins activate GATA-6 and induce differentiated VSMCs

  11. Statins activate GATA-6 and induce differentiated vascular smooth muscle cells

    Energy Technology Data Exchange (ETDEWEB)

    Wada, Hiromichi [Division of Translational Research, National Hospital Organization Kyoto Medical Center, 1-1 Mukaihata-cho, Fukakusa, Fushimi-ku, Kyoto 612-8555 (Japan); Abe, Mitsuru; Ono, Koh [Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Morimoto, Tatsuya; Kawamura, Teruhisa; Takaya, Tomohide [Division of Translational Research, National Hospital Organization Kyoto Medical Center, 1-1 Mukaihata-cho, Fukakusa, Fushimi-ku, Kyoto 612-8555 (Japan); Satoh, Noriko [Division of Metabolic Research, National Hospital Organization Kyoto Medical Center, Kyoto (Japan); Fujita, Masatoshi [Department of Human Health Sciences, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Kita, Toru [Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto (Japan); Shimatsu, Akira [Clinical Research Institute, National Hospital Organization Kyoto Medical Center, Kyoto (Japan); Hasegawa, Koji [Division of Translational Research, National Hospital Organization Kyoto Medical Center, 1-1 Mukaihata-cho, Fukakusa, Fushimi-ku, Kyoto 612-8555 (Japan)

    2008-10-03

    The beneficial effects of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins) beyond cholesterol lowering involve their direct actions on vascular smooth muscle cells (VSMCs). However, the effects of statins on phenotypic modulation of VSMCs are unknown. We herein show that simvastatin (Sm) and atorvastatin (At) inhibited DNA synthesis in human aortic VSMCs dose-dependently, while cell toxicity was not observed below the concentration of 1 {mu}M of Sm or 100 nM of At. Stimulating proliferative VSMCs with Sm or At induced the expression of SM-{alpha}-actin and SM-MHC, highly specific markers of differentiated phenotype. Sm up-regulated the binding activity of GATA-6 to SM-MHC GATA site and activated the transfected SM-MHC promoter in proliferative VSMCs, while mutating the GATA-6 binding site abolished this activation. Geranylgeranylpyrophosphate (10 {mu}M), an inhibitor of Rho family proteins, abolished the statin-mediated induction of the differentiated phenotype in VSMCs. These findings suggest that statins activate GATA-6 and induce differentiated VSMCs.

  12. GATA-dependent regulation of TPO-induced c-mpl gene expression during megakaryopoiesis.

    Science.gov (United States)

    Sunohara, Masataka; Morikawa, Shigeru; Fuse, Akira; Sato, Iwao

    2014-01-01

    Thrombopoietin (TPO) and its receptor, c-Mpl, play the crucial role during megakaryocytopoiesis. Previously, we have shown that the promoter activity of c-mpl induced by TPO is modulated by transcription through a PKC-dependent pathway and that GATA(-77) is involved as a positive regulatory element in TPO-induced c-mpl gene expression in the megakaryoblastic CMK cells. In this research, to examine participating possibility of GATA promoter element in TPO- induced c-mpl gene expression through a PKC-independent pathway, the promoter activity of site-directed mutagenesis and the effect of potein kinase C modulator were measured by a transient transfection assay system. Together with our previous results on the TPO-induced c-mpl promoter, this study indicates destruction of -77GATA in c-mpl promoter decreased the activity by 47.3% under existence of GF109203. These results suggest that GATA promoter element plays significant role in TPO-induced c-mpl gene expression through a PKC-independent pathway.

  13. hHO-1 combined with GATA-4 transduction promotes myocardial transdifferentiation and anti- apoptosis of rat mesenchymal stem cells

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    Ning-bo DENG

    2017-06-01

    Full Text Available Objectives To explore if the rat bone marrow mesenchymal stem cells (BMSCs modified by human heme oxygenase 1 (hHO-1 gene combined with GATA-4 gene may promote the ability of anti-apoptosis and myocardial transdifferentiation in vitro in hypoxia ischemic environment. Methods The rat BMSCs were isolated and cultured by whole bone marrow adherence and identified in vitro, and then were transfected with recombinant adenovirus; Western blotting was used to determinate the optimal time of gene expression; the genetically modified BMSCs were taken to hypoxia serum-free conditions simulating ischemia hypoxia microenvironment in vivo; CCK-8 kit and trypan blue staining were performed to detect the 12, 24, 48 and 72h survival rates in hypoxia ischemia respectively; flow cytometry was used to detect the apoptosis of BMSCs in hypoxia ischemia for 24h. The cardiomyocyte-specific cardiac troponin I (cTnI was detected by Western blotting and cellular immunofluorescence. Results The 12, 24, 48 and 72h survival rates were higher in hHO-1+GATA-4 group cultured in ischemia and hypoxia condition than in hHO-1 group (P<0.05 and GATA-4 group (P<0.05. After 24h cultivation in ischemia hypoxia condition, the apoptotic rates were lower in hHO-1+GATA-4 group than in hHO-1 group (P<0.05 and GATA-4 group (P<0.05. No significant difference existed in cTnI expressions between GATA-4 group and hHO-1+GATA-4 group. Conclusion Compared with transfection of hHO-1 or GATA-4 single gene, hHO-1 combined with GATA-4 transduction can significantly increase the survival rate of BMSCs in hypoxia ischemic condition, but myocardial transdifferentiation does not increase significantly. DOI: 10.11855/j.issn.0577-7402.2017.04.08

  14. MGMT, GATA6, CD81, DR4, and CASP8 gene promoter methylation in glioblastoma

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    Skiriute Daina

    2012-06-01

    Full Text Available Abstract Background Methylation of promoter region is the major mechanism affecting gene expression in tumors. Recent methylome studies of brain tumors revealed a list of new epigenetically modified genes. Our aim was to study promoter methylation of newly identified epigenetically silenced genes together with already known epigenetic markers and evaluate its separate and concomitant role in glioblastoma genesis and patient outcome. Methods The methylation status of MGMT, CD81, GATA6, DR4, and CASP8 in 76 patients with primary glioblastomas was investigated. Methylation-specific PCR reaction was performed using bisulfite treated DNA. Evaluating glioblastoma patient survival time after operation, patient data and gene methylation effect on survival was estimated using survival analysis. Results The overwhelming majority (97.3% of tumors were methylated in at least one of five genes tested. In glioblastoma specimens gene methylation was observed as follows: MGMT in 51.3%, GATA6 in 68.4%, CD81 in 46.1%, DR4 in 41.3% and CASP8 in 56.8% of tumors. Methylation of MGMT was associated with younger patient age (p CASP8 with older (p MGMT methylation was significantly more frequent event in patient group who survived longer than 36 months after operation (p CASP8 was more frequent in patients who survived shorter than 36 months (p MGMT, GATA6 and CASP8 as independent predictors for glioblastoma patient outcome (p MGMT and GATA6 were independent predictors for patient survival in younger patients’ group, while there were no significant associations observed in older patients’ group when adjusted for therapy. Conclusions High methylation frequency of tested genes shows heterogeneity of glioblastoma epigenome and the importance of MGMT, GATA6 and CASP8 genes methylation in glioblastoma patient outcome.

  15. Assessment of the role of transcript for GATA-4 as a marker of unfavorable outcome in human adrenocortical neoplasms

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    Martin Regina M

    2004-07-01

    Full Text Available Abstract Background Malignant neoplasia of the adrenal cortex is usually associated with very poor prognosis. When adrenocortical neoplasms are diagnosed in the early stages, distinction between carcinoma and adenoma can be very difficult to accomplish, since there is yet no reliable marker to predict tumor recurrence or dissemination. GATA transcription factors play an essential role in the developmental control of cell fate, cell proliferation and differentiation, organ morphogenesis, and tissue-specific gene expression. Normal mouse adrenal cortex expresses GATA-6 while its malignant counterpart only expresses GATA-4. The goal of the present study was to assess whether this reciprocal change in the expression of GATA factors might be relevant for predicting the prognosis of human adrenocortical neoplasms. Since human adrenal cortices express luteinizing hormone (LH/hCG receptor and the gonadotropins are known to up-regulate GATA-4 in gonadal tumor cell lines, we also studied the expression of LH/hCG receptor. Methods We conducted a study on 13 non-metastasizing (NM and 10 metastasizing/recurrent (MR tumors obtained from a group of twenty-two adult and pediatric patients. The expression of GATA-4, GATA-6, and LH/hCG receptor (LHR in normal and tumoral human adrenal cortices was analysed using reverse transcriptase-polymerase chain reaction (RT-PCR complemented by dot blot hybridization. Results Messenger RNA for GATA-6 was detected in normal adrenal tissue, as well as in the totality of NM and MR tumors. GATA-4, by its turn, was detected in normal adrenal tissue, in 11 out of 13 NM tumors, and in 9 of the 10 MR tumors, with larger amounts of mRNA found among those presenting aggressive clinical behavior. Transcripts for LH receptor were observed both in normal tissue and neoplasms. A more intense LHR transcript accumulation was observed on those tumors with better clinical outcome. Conclusion Our data suggest that the expression of GATA-6 in

  16. Organization and differential expression of the GACA/GATA tagged somatic and spermatozoal transcriptomes in Buffalo Bubalus bubalis

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    Srivastava Jyoti

    2008-03-01

    Full Text Available Abstract Background Simple sequence repeats (SSRs of GACA/GATA have been implicated with differentiation of sex-chromosomes and speciation. However, the organization of these repeats within genomes and transcriptomes, even in the best characterized organisms including human, remains unclear. The main objective of this study was to explore the buffalo transcriptome for its association with GACA/GATA repeats, and study the structural organization and differential expression of the GACA/GATA repeat tagged transcripts. Moreover, the distribution of GACA and GATA repeats in the prokaryotic and eukaryotic genomes was studied to highlight their significance in genome evolution. Results We explored several genomes and transcriptomes, and observed total absence of these repeats in the prokaryotes, with their gradual accumulation in higher eukaryotes. Further, employing novel microsatellite associated sequence amplification (MASA approach using varying length oligos based on GACA and GATA repeats; we identified and characterized 44 types of known and novel mRNA transcripts tagged with these repeats from different somatic tissues, gonads and spermatozoa of water buffalo Bubalus bubalis. GACA was found to be associated with higher number of transcripts compared to that with GATA. Exclusive presence of several GACA-tagged transcripts in a tissue or spermatozoa, and absence of the GATA-tagged ones in lung/heart highlights their tissue-specific significance. Of all the GACA/GATA tagged transcripts, ~30% demonstrated inter-tissue and/or tissue-spermatozoal sequence polymorphisms. Significantly, ~60% of the GACA-tagged and all the GATA-tagged transcripts showed highest or unique expression in the testis and/or spermatozoa. Moreover, ~75% GACA-tagged and all the GATA-tagged transcripts were found to be conserved across the species. Conclusion Present study is a pioneer attempt exploring GACA/GATA tagged transcriptome in any mammalian species highlighting their

  17. Preferential expression of NY-BR-1 and GATA-3 in male breast cancer.

    Science.gov (United States)

    Biserni, Giovanni Battista; Di Oto, Enrico; Moskovszky, Linda Eszter; Foschini, Maria Pia; Varga, Zsuzsanna

    2018-02-01

    Male breast cancer is an uncommon disease often discovered in advanced stage; thus, in the setting of metastatic adenocarcinoma, breast origin must be taken to account. Breast markers as NY-BR-1, GATA-3, mammaglobin, and BRST-2 are established tools for labelling primary and metastatic female breast cancer; however, none of them has been sufficiently studied in male breast cancer. The aim of this study was to analyze the expression of these markers in male breast cancer. Thirty consecutive cases of male breast cancer and eight loco-regional metastases were re-revaluated, assembled in tissue micro array (TMA), and stained with immunohistochemistry (IHC) for NY-BR-1, GATA-3, mammaglobin, and BRST-2. The IHC stains were scored either positive or negative. In addition, concordant expression patterns of primary tumors and matched metastasis were noted. 30 of 30 (100%) primary tumors and 8 of 8 (100%) metastases were positive for NY-BR-1. 30 of 30 (100%) primary tumors and 6 of 8 (75%) metastases were positive for GATA-3. 22 of 30 (73.3%) primary tumors and 6 of 8 (75%) metastases were positive for Mammaglobin. 18 of 30 (60%) primary tumors and 5 of 8 (62.5%) metastases were positive for BRST-2. Differences in staining percentage were not significant with Fisher's exact test. We found a high sensitivity for all the markers analyzed. Moreover, the expression of NY-BR-1 and GATA-3 seemed the most effective for labelling male breast cancer in primary and metastatic setting.

  18. Anagrelide represses GATA-1 and FOG-1 expression without interfering with thrombopoietin receptor signal transduction.

    Science.gov (United States)

    Ahluwalia, M; Donovan, H; Singh, N; Butcher, L; Erusalimsky, J D

    2010-10-01

     Anagrelide is a selective inhibitor of megakaryocytopoiesis used to treat thrombocytosis in patients with chronic myeloproliferative disorders. The effectiveness of anagrelide in lowering platelet counts is firmly established, but its primary mechanism of action remains elusive.  Here, we have evaluated whether anagrelide interferes with the major signal transduction cascades stimulated by thrombopoietin in the hematopoietic cell line UT-7/mpl and in cultured CD34(+) -derived human hematopoietic cells. In addition, we have used quantitative mRNA expression analysis to assess whether the drug affects the levels of known transcription factors that control megakaryocytopoiesis.  In UT-7/mpl cells, anagrelide (1μm) did not interfere with MPL-mediated signaling as monitored by its lack of effect on JAK2 phosphorylation. Similarly, the drug did not affect the phosphorylation of STAT3, ERK1/2 or AKT in either UT-7/mpl cells or primary hematopoietic cells. In contrast, during thrombopoietin-induced megakaryocytic differentiation of normal hematopoietic cultures, anagrelide (0.3μm) reduced the rise in the mRNA levels of the transcription factors GATA-1 and FOG-1 as well as those of the downstream genes encoding FLI-1, NF-E2, glycoprotein IIb and MPL. However, the drug showed no effect on GATA-2 or RUNX-1 mRNA expression. Furthermore, anagrelide did not diminish the rise in GATA-1 and FOG-1 expression during erythropoietin-stimulated erythroid differentiation. Cilostamide, an exclusive and equipotent phosphodiesterase III (PDEIII) inhibitor, did not alter the expression of these genes.  Anagrelide suppresses megakaryocytopoiesis by reducing the expression levels of GATA-1 and FOG-1 via a PDEIII-independent mechanism that is differentiation context-specific and does not involve inhibition of MPL-mediated early signal transduction events. © 2010 International Society on Thrombosis and Haemostasis.

  19. Spectrum of myeloid neoplasms and immune deficiency associated with germline GATA2 mutations

    OpenAIRE

    Mir, Muhammad A; Kochuparambil, Samith T; Abraham, Roshini S; Rodriguez, Vilmarie; Howard, Matthew; Hsu, Amy P; Jackson, Amie E; Holland, Steven M; Patnaik, Mrinal M

    2015-01-01

    Guanine-adenine-thymine-adenine 2 (GATA2) mutated disorders include the recently described MonoMAC syndrome (Monocytopenia and Mycobacterium avium complex infections), DCML (dendritic cell, monocyte, and lymphocyte deficiency), familial MDS/AML (myelodysplastic syndrome/acute myeloid leukemia) (myeloid neoplasms), congenital neutropenia, congenital lymphedema (Emberger's syndrome), sensorineural deafness, viral warts, and a spectrum of aggressive infections seen across all age groups. While c...

  20. Regulation of Nitrogen Metabolism by GATA Zinc Finger Transcription Factors in Yarrowia lipolytica

    OpenAIRE

    Pomraning, Kyle R.; Bredeweg, Erin L.; Baker, Scott E.

    2017-01-01

    ABSTRACT Fungi accumulate lipids in a manner dependent on the quantity and quality of the nitrogen source on which they are growing. In the oleaginous yeast Yarrowia lipolytica, growth on a complex source of nitrogen enables rapid growth and limited accumulation of neutral lipids, while growth on a simple nitrogen source promotes lipid accumulation in large lipid droplets. Here we examined the roles of nitrogen catabolite repression and its regulation by GATA zinc finger transcription factors...

  1. Investigating the DNA-binding ability of GATA-1-N-terminal zinc finger

    International Nuclear Information System (INIS)

    Wong, R.; Newton, A.; Crossley, M.; Mackay, J.

    2001-01-01

    Erythroid transcription factor GATA-1 interacts with both DNA and other proteins through its zinc finger domains (ZnFs). While it has been known for me time that the C-terminal ZnF binds DNA at GATA sites, only recently has it been observed that the N-terminal finger (NF) is capable of interacting with GATC sites. Further, a number of naturally occurring mutations in NF (V205M, G208S, R216Q, D218G) that lead to anaemia and thrombocytopenia have been identified. We are interested in characterising the NF-DNA interaction and determining the effects of mutation upon this interaction. Using nuclear magnetic resonance (NMR) spectroscopy, we have observed an interaction between recombinant NF and a 16-mer DNA duplex containing a core GATC sequence. This result forms the basis from which residues in NF involved in DNA binding can be identified, and work is being carried out to improve the quality of the NMR data with the aim of determining the solution structure of the NF-DNA complex. The DNA-binding affinity of both wild-type and mutant NFs mentioned above is also being investigated using isothermal titration calorimetry. These data suggest that the strength of the interaction between NF and the 16-mer DNA duplex is in the sub-micromolar range, and comparisons between the DNA-binding affinities of the NF mutants are being made. Together, these studies will help us to understand how GATA-1 acts as a transcriptional regulator and how mutations in NF domain of GATA-1 may lead to blood disorders

  2. The GATA transcription factor egl-27 delays aging by promoting stress resistance in Caenorhabditis elegans.

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    Xiao Xu

    Full Text Available Stress is a fundamental aspect of aging, as accumulated damage from a lifetime of stress can limit lifespan and protective responses to stress can extend lifespan. In this study, we identify a conserved Caenorhabditis elegans GATA transcription factor, egl-27, that is involved in several stress responses and aging. We found that overexpression of egl-27 extends the lifespan of wild-type animals. Furthermore, egl-27 is required for the pro-longevity effects from impaired insulin/IGF-1 like signaling (IIS, as reduced egl-27 activity fully suppresses the longevity of worms that are mutant for the IIS receptor, daf-2. egl-27 expression is inhibited by daf-2 and activated by pro-longevity factors daf-16/FOXO and elt-3/GATA, suggesting that egl-27 acts at the intersection of IIS and GATA pathways to extend lifespan. Consistent with its role in IIS signaling, we found that egl-27 is involved in stress response pathways. egl-27 expression is induced in the presence of multiple stresses, its targets are significantly enriched for many types of stress genes, and altering levels of egl-27 itself affects survival to heat and oxidative stress. Finally, we found that egl-27 expression increases between young and old animals, suggesting that increased levels of egl-27 in aged animals may act to promote stress resistance. These results identify egl-27 as a novel factor that links stress and aging pathways.

  3. Association of autoimmune Addison's disease with alleles of STAT4 and GATA3 in European cohorts.

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    Anna L Mitchell

    Full Text Available Gene variants known to contribute to Autoimmune Addison's disease (AAD susceptibility include those at the MHC, MICA, CIITA, CTLA4, PTPN22, CYP27B1, NLRP-1 and CD274 loci. The majority of the genetic component to disease susceptibility has yet to be accounted for.To investigate the role of 19 candidate genes in AAD susceptibility in six European case-control cohorts.A sequential association study design was employed with genotyping using Sequenom iPlex technology. In phase one, 85 SNPs in 19 genes were genotyped in UK and Norwegian AAD cohorts (691 AAD, 715 controls. In phase two, 21 SNPs in 11 genes were genotyped in German, Swedish, Italian and Polish cohorts (1264 AAD, 1221 controls. In phase three, to explore association of GATA3 polymorphisms with AAD and to determine if this association extended to other autoimmune conditions, 15 SNPs in GATA3 were studied in UK and Norwegian AAD cohorts, 1195 type 1 diabetes patients from Norway, 650 rheumatoid arthritis patients from New Zealand and in 283 UK Graves' disease patients. Meta-analysis was used to compare genotype frequencies between the participating centres, allowing for heterogeneity.We report significant association with alleles of two STAT4 markers in AAD cohorts (rs4274624: P = 0.00016; rs10931481: P = 0.0007. In addition, nominal association of AAD with alleles at GATA3 was found in 3 patient cohorts and supported by meta-analysis. Association of AAD with CYP27B1 alleles was also confirmed, which replicates previous published data. Finally, nominal association was found at SNPs in both the NF-κB1 and IL23A genes in the UK and Italian cohorts respectively.Variants in the STAT4 gene, previously associated with other autoimmune conditions, confer susceptibility to AAD. Additionally, we report association of GATA3 variants with AAD: this adds to the recent report of association of GATA3 variants with rheumatoid arthritis.

  4. All-trans retinoic acid directs urothelial specification of murine embryonic stem cells via GATA4/6 signaling mechanisms.

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    Joshua R Mauney

    2010-07-01

    Full Text Available The urinary bladder and associated tract are lined by the urothelium, a transitional epithelium that acts as a specialized permeability barrier that protects the underlying tissue from urine via expression of a highly specific group of proteins known as the uroplakins (UP. To date, our understanding of the developmental processes responsible for urothelial differentiation has been hampered due to the lack of suitable models. In this study, we describe a novel in vitro cell culture system for derivation of urothelial cells from murine embryonic stem cells (ESCs following cultivation on collagen matrices in the presence all trans retinoic acid (RA. Upon stimulation with micromolar concentrations of RA, ESCs significantly downregulated the pluripotency factor OCT-4 but markedly upregulated UP1A, UP1B, UP2, UP3A, and UP3B mRNA levels in comparison to naïve ESCs and spontaneously differentiating controls. Pan-UP protein expression was associated with both p63- and cytokeratin 20-positive cells in discrete aggregating populations of ESCs following 9 and 14 days of RA stimulation. Analysis of endodermal transcription factors such as GATA4 and GATA6 revealed significant upregulation and nuclear enrichment in RA-treated UP2-GFP+ populations. GATA4-/- and GATA6-/- transgenic ESC lines revealed substantial attenuation of RA-mediated UP expression in comparison to wild type controls. In addition, EMSA analysis revealed that RA treatment induced formation of transcriptional complexes containing GATA4/6 on both UP1B and UP2 promoter fragments containing putative GATA factor binding sites. Collectively, these data suggest that RA mediates ESC specification toward a urothelial lineage via GATA4/6-dependent processes.

  5. miR-200c and GATA binding protein 4 regulate human embryonic stem cell renewal and differentiation

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    Hsiao-Ning Huang

    2014-03-01

    Full Text Available Human embryonic stem cells (hESCs are functionally unique for their self-renewal ability and pluripotency, but the molecular mechanisms giving rise to these properties are not fully understood. hESCs can differentiate into embryoid bodies (EBs containing ectoderm, mesoderm, and endoderm. In the miR-200 family, miR-200c was especially enriched in undifferentiated hESCs and significantly downregulated in EBs. The knockdown of the miR-200c in hESCs downregulated Nanog expression, upregulated GATA binding protein 4 (GATA4 expression, and induced hESC apoptosis. The knockdown of GATA4 rescued hESC apoptosis induced by downregulation of miR-200c. miR-200c directly targeted the 3′-untranslated region of GATA4. Interestingly, the downregulation of GATA4 significantly inhibited EB formation in hESCs. Overexpression of miR-200c inhibited EB formation and repressed the expression of ectoderm, endoderm, and mesoderm markers, which could partially be rescued by ectopic expression of GATA4. Fibroblast growth factor (FGF and activin A/nodal can sustain hESC renewal in the absence of feeder layer. Inhibition of transforming growth factor-β (TGF-β/activin A/nodal signaling by SB431542 treatment downregulated the expression of miR-200c. Overexpression of miR-200c partially rescued the expression of Nanog/phospho-Smad2 that was downregulated by SB431542 treatment. Our observations have uncovered novel functions of miR-200c and GATA4 in regulating hESC renewal and differentiation.

  6. Angiotensin II induces apoptosis in intestinal epithelial cells through the AT2 receptor, GATA-6 and the Bax pathway

    International Nuclear Information System (INIS)

    Sun, Lihua; Wang, Wensheng; Xiao, Weidong; Liang, Hongyin; Yang, Yang; Yang, Hua

    2012-01-01

    Highlights: ► Ang II-induced apoptosis in intestinal epithelial cell through AT2 receptor. ► The apoptosis process involves in the Bax/Bcl-2 intrinsic pathway. ► GATA-6 short hairpin RNA reduced Bax expression, but not Bcl-2. ► GATA-6 may play a critical role in apoptosis in response to the Ang II challenge. -- Abstract: Angiotensin II (Ang II) has been shown to play an important role in cell apoptosis. However, the mechanisms of Ang-II-induced apoptosis in intestinal epithelial cells are not fully understood. GATA-6 is a zinc finger transcription factor expressed in the colorectal epithelium, which directs cell proliferation, differentiation and apoptosis. In the present study we investigated the underlying mechanism of which GATA-6 affects Ang-II induced apoptosis in intestinal epithelial cells. The in vitro intestinal epithelial cell apoptosis model was established by co-culturing Caco-2 cells with Ang II. Pretreatment with Angiotensin type 2 (AT2) receptor antagonist, PD123319, significantly reduced the expression of Bax and prevented the Caco-2 cells apoptosis induced by Ang II. In addition, Ang II up-regulated the expression of GATA-6. Interestingly, GATA-6 short hairpin RNA prevented Ang II-induced intestinal epithelial cells apoptosis and reduced the expression of Bax, but not Bcl-2. Taken together, the present study suggests that Angiotensin II promotes apoptosis in intestinal epithelial cells through GATA-6 and the Bax pathway in an AT2 receptor-dependent manner.

  7. Novel mutation of GATA4 gene in Kurdish population of Iran with nonsyndromic congenital heart septals defects.

    Science.gov (United States)

    Soheili, Fariborz; Jalili, Zahra; Rahbar, Mahtab; Khatooni, Zahed; Mashayekhi, Amir; Jafari, Hossein

    2018-03-01

    The mutations in GATA4 gene induce inherited atrial and ventricular septation defects, which is the most frequent forms of congenital heart defects (CHDs) constituting about half of all cases. We have performed High resolution melting (HRM) mutation scanning of GATA4 coding exons of nonsyndrome 100 patients as a case group including 39 atrial septal defects (ASD), 57 ventricular septal defects (VSD) and four patients with both above defects and 50 healthy individuals as a control group. Our samples are categorized according to their HRM graph. The genome sequencing has been done for 15 control samples and 25 samples of patients whose HRM analysis were similar to healthy subjects for each exon. The PolyPhen-2 and MUpro have been used to determine the causative possibility and structural stability prediction of GATA4 sequence variation. The HRM curve analysis exhibit that 21 patients and 3 normal samples have deviated curves for GATA4 coding exons. Sequencing analysis has revealed 12 nonsynonymous mutations while all of them resulted in stability structure of protein 10 of them are pathogenic and 2 of them are benign. Also we found two nucleotide deletions which one of them was novel and one new indel mutation resulting in frame shift mutation, and 4 synonymous variations or polymorphism in 6 of patients and 3 of normal individuals. Six or about 50% of these nonsynonymous mutations have not been previously reported. Our results show that there is a spectrum of GATA4 mutations resulting in septal defects. © 2018 Wiley Periodicals, Inc.

  8. Fisiología reproductiva y control de los ciclos estrales en la gata doméstica

    OpenAIRE

    Giménez, F.; Stornelli, María Cecilia; Savignone, César A.; Tittarelli, Claudia Marcela; Sota, Rodolfo Luzbel de la; Stornelli, María Alejandra

    2006-01-01

    La gata doméstica es poliéstrica estacional, con ovulación inducida por el coito. Sin embargo, la ovulación espontánea puede ocurrir en algunas hembras. El celo ocurre con un intervalo de 14 a 19 días en aquellas gatas expuestas a un fotoperíodo largo (14 horas luz diarias). El ciclo estral felino se divide en cuatro períodos: proestro, estro, interestro y anestro. Cada etapa presenta ciertas particularidades que diferencian una de otra. Existen diferentes métodos para prevenir tanto la ocurr...

  9. Tumor de células da granulosa com metástases numa gata

    Directory of Open Access Journals (Sweden)

    Márcia Regina Giacóia

    1999-01-01

    Full Text Available As características clínicas, macroscópicas e microscópicas de um tumor de células da granulosa no ovário de uma gata de 2 anos de idade são descritas neste trabalho. Essa neoplasia é rara em gatos, principalmente devido à idade apresentada pelo animal. O diagnóstico foi feito clinicamente através de exame ultra-sonográfico. O tumor, uma massa cística na região do ovário esquerdo, metastatizou para o epíploo e para os pulmões. As células tumorais expressaram vimentina e arranjavam-se num padrão sarcomatoso e difuso, sustentado por fino estroma fibrovascular. A presença de sinais clínicos, como perda de pêlos e repetição de estros, é indicativa de síndrome paraneoplásica ortoendócrina, devido à excessiva estimulação estrogênica. A existência desse tipo de tumor deve ser considerada no diagnóstico diferencial de distúrbios comportamentais em gatas.

  10. Wnt3 and Gata4 regulate axon regeneration in adult mouse DRG neurons.

    Science.gov (United States)

    Duan, Run-Shan; Liu, Pei-Pei; Xi, Feng; Wang, Wei-Hua; Tang, Gang-Bin; Wang, Rui-Ying; Saijilafu; Liu, Chang-Mei

    2018-05-05

    Neurons in the adult central nervous system (CNS) have a poor intrinsic axon growth potential after injury, but the underlying mechanisms are largely unknown. Wingless-related mouse mammary tumor virus integration site (WNT) family members regulate neural stem cell proliferation, axon tract and forebrain development in the nervous system. Here we report that Wnt3 is an important modulator of axon regeneration. Downregulation or overexpression of Wnt3 in adult dorsal root ganglion (DRG) neurons enhances or inhibits their axon regeneration ability respectively in vitro and in vivo. Especially, we show that Wnt3 modulates axon regeneration by repressing mRNA translation of the important transcription factor Gata4 via binding to the three prime untranslated region (3'UTR). Downregulation of Gata4 could restore the phenotype exhibited by Wnt3 downregulation in DRG neurons. Taken together, these data indicate that Wnt3 is a key intrinsic regulator of axon growth ability of the nervous system. Copyright © 2018 Elsevier Inc. All rights reserved.

  11. RhIL-11 treatment normalized Th1/Th2 and T-bet/GATA-3 imbalance in in human immune thrombocytopenic purpura (ITP).

    Science.gov (United States)

    Lin, Ying; Zhou, Xieming; Guo, Wenjian; Li, Qianqian; Pan, Xiahui; Bao, Yunhua; He, Muqing; Zhu, Baoling; Lin, Xiaoji; Jin, Limin; Yao, Rongxin

    2016-09-01

    Immune thrombocytopenia (ITP) is an autoimmune hemorrhagic disorder characterized by reduction in platelet counts. T helper 1 (Th1) cells polarization with an increased shift of Th1/Th2 ratio has been reported in ITP. This shift is associated with transcription factor T-box expressed in T cells (T-bet) upregulation and GATA-binding protein 3 (GATA-3) downregulation, leading to an increased T-bet/GATA-3 ratio. Our previous in vitro study showed that recombinant human interleukin-11 (rhIL-11) could normalize Th1/Th2 imbalance in the peripheral blood mononuclear cells (PBMCs) isolated from adult ITP patients, which co-occurred with T-bet/GATA-3 ratio restoration. In this report, we investigated whether rhIL-11 had therapeutic effect in clinical ITP patients and whether rhIL-11 treatment could normalize Th1/Th2 and T-bet/GATA-3 levels in vivo. We found rhIL-11 treatment had a response rate of 67.7% and significantly decreased Th1 and T-bet levels but increased Th2 and GATA-3 levels in ITP patients who showed good response, normalizing Th1/Th2 and T-bet/GATA-3 ratios similar to that in healthy controls. Thus our study suggested rhIL-11 was effective with tolerable adverse effects in ITP. The treatment strategy warrants further clinical investigation. Copyright © 2016 Elsevier B.V. All rights reserved.

  12. Multiplex Ligation-Dependent Probe Amplification Analysis of GATA4 Gene Copy Number Variations in Patients with Isolated Congenital Heart Disease

    Directory of Open Access Journals (Sweden)

    Valentina Guida

    2010-01-01

    Full Text Available GATA4 mutations are found in patients with different isolated congenital heart defects (CHDs, mostly cardiac septal defects and tetralogy of Fallot. In addition, GATA4 is supposed to be the responsible gene for the CHDs in the chromosomal 8p23 deletion syndrome, which is recognized as a malformation syndrome with clinical symptoms of facial anomalies, microcephaly, mental retardation, and congenital heart defects. Thus far, no study has been carried out to investigate the role of GATA4 copy number variations (CNVs in non-syndromic CHDs. To explore the possible occurrence of GATA4 gene CNVs in isolated CHDs, we analyzed by multiplex ligation-dependent probe amplification (MLPA a cohort of 161 non-syndromic patients with cardiac anomalies previously associated with GATA4 gene mutations. The patients were mutation-negative for GATA4, NKX2.5, and FOG2 genes after screening with denaturing high performance liquid chromatography. MLPA analysis revealed that normalized MLPA signals were all found within the normal range values for all exons in all patients, excluding a major contribution of GATA4 gene CNVs in CHD pathogenesis.

  13. Cyclin D-Cdk4 is regulated by GATA-1 and required for megakaryocyte growth and polyploidization.

    Science.gov (United States)

    Muntean, Andrew G; Pang, Liyan; Poncz, Mortimer; Dowdy, Steven F; Blobel, Gerd A; Crispino, John D

    2007-06-15

    Endomitosis is a unique form of cell cycle used by megakaryocytes, in which the latter stages of mitosis are bypassed so that the cell can increase its DNA content and size. Although several transcription factors, including GATA-1 and RUNX-1, have been implicated in this process, the link between transcription factors and polyploidization remains undefined. Here we show that GATA-1-deficient megakaryocytes, which display reduced size and polyploidization, express nearly 10-fold less cyclin D1 and 10-fold increased levels of p16 compared with their wild-type counterparts. We further demonstrate that cyclin D1 is a direct GATA-1 target in megakaryocytes, but not erythroid cells. Restoration of cyclin D1 expression, when accompanied by ectopic overexpression of its partner Cdk4, resulted in a dramatic increase in megakaryocyte size and DNA content. However, terminal differentiation was not rescued. Of note, polyploidization was only modestly reduced in cyclin D1-deficient mice, likely due to compensation by elevated cyclin D3 expression. Finally, consistent with an additional defect conferred by increased levels of p16, inhibition of cyclin D-Cdk4 complexes with a TAT-p16 fusion peptide significantly blocked polyploidization of wild-type megakaryocytes. Together, these data show that GATA-1 controls growth and polyploidization by regulating cyclin D-Cdk4 kinase activity.

  14. Cyclin D–Cdk4 is regulated by GATA-1 and required for megakaryocyte growth and polyploidization

    Science.gov (United States)

    Muntean, Andrew G.; Pang, Liyan; Poncz, Mortimer; Dowdy, Steven F.; Blobel, Gerd A.

    2007-01-01

    Endomitosis is a unique form of cell cycle used by megakaryocytes, in which the latter stages of mitosis are bypassed so that the cell can increase its DNA content and size. Although several transcription factors, including GATA-1 and RUNX-1, have been implicated in this process, the link between transcription factors and polyploidization remains undefined. Here we show that GATA-1–deficient megakaryocytes, which display reduced size and polyploidization, express nearly 10-fold less cyclin D1 and 10-fold increased levels of p16 compared with their wild-type counterparts. We further demonstrate that cyclin D1 is a direct GATA-1 target in megakaryocytes, but not erythroid cells. Restoration of cyclin D1 expression, when accompanied by ectopic overexpression of its partner Cdk4, resulted in a dramatic increase in megakaryocyte size and DNA content. However, terminal differentiation was not rescued. Of note, polyploidization was only modestly reduced in cyclin D1–deficient mice, likely due to compensation by elevated cyclin D3 expression. Finally, consistent with an additional defect conferred by increased levels of p16, inhibition of cyclin D-Cdk4 complexes with a TAT-p16 fusion peptide significantly blocked polyploidization of wild-type megakaryocytes. Together, these data show that GATA-1 controls growth and polyploidization by regulating cyclin D-Cdk4 kinase activity. PMID:17317855

  15. A novel GATA3 nonsense mutation in a newly diagnosed adult patient of hypoparathyroidism, deafness, and renal dysplasia (HDR) syndrome.

    Science.gov (United States)

    Nanba, Kazutaka; Usui, Takeshi; Nakamura, Michikazu; Toyota, Yuko; Hirota, Keisho; Tamanaha, Tamiko; Kawashima, Sachiko-Tsukamoto; Nakao, Kanako; Yuno, Akiko; Tagami, Tetsuya; Naruse, Mitsuhide; Shimatsu, Akira

    2013-01-01

    Hypoparathyroidism, deafness, and renal dysplasia (HDR) syndrome is an autosomal dominant disorder caused by a GATA3 gene mutation. Here we report a novel mutation of GATA3 in a patient diagnosed with HDR syndrome at the age of 58 with extensive intracranial calcification. A 58-year-old Japanese man showed severe hypocalcemia and marked calcification in the basal ganglia, cerebellum, deep white matter, and gray-white junction on computed tomography (CT). The serum intact parathyroid hormone level was relatively low against low serum calcium concentration. The patient had been diagnosed with bilateral sensorineural deafness in childhood and had a family history of hearing disorders. Imaging studies revealed no renal anomalies. The patient was diagnosed with HDR syndrome, and genetic testing was performed. Genetic analysis of GATA3 showed a novel nonsense mutation at codon 198 (S198X) in exon 3. The S198X mutation leads to a loss of two zinc finger deoxyribonucleic acid (DNA) binding domains and is considered to be responsible for HDR syndrome. We identified a novel nonsense mutation of GATA3 in an adult patient with HDR syndrome who showed extensive intracranial calcification.

  16. Association of GATA2 Deficiency With Severe Primary Epstein-Barr Virus (EBV) Infection and EBV-associated Cancers.

    Science.gov (United States)

    Cohen, Jeffrey I; Dropulic, Lesia; Hsu, Amy P; Zerbe, Christa S; Krogmann, Tammy; Dowdell, Kennichi; Hornung, Ronald L; Lovell, Jana; Hardy, Nancy; Hickstein, Dennis; Cowen, Edward W; Calvo, Katherine R; Pittaluga, Stefania; Holland, Steven M

    2016-07-01

    Most patients infected with Epstein-Barr virus (EBV) are asymptomatic, have nonspecific symptoms, or have self-limiting infectious mononucleosis. EBV, however, may result in severe primary disease or cancer. We report EBV diseases associated with GATA2 deficiency at one institution and describe the hematology, virology, and cytokine findings. Seven patients with GATA2 deficiency developed severe EBV disease. Three presented with EBV infectious mononucleosis requiring hospitalization, 1 had chronic active EBV disease (B-cell type), 1 had EBV-associated hydroa vacciniforme-like lymphoma with hemophagocytic lymphohistiocytosis, and 2 had EBV-positive smooth muscle tumors. Four of the 7 patients had severe warts and 3 had disseminated nontuberculous mycobacterial infections. All of the patients had low numbers of monocytes, B cells, CD4 T cells, and natural killer cells. All had elevated levels of EBV in the blood; 2 of 3 patients tested had expression of the EBV major immediate-early gene in the blood indicative of active EBV lytic infection. Mean plasma levels of tumor necrosis factor α, interferon γ, and interferon gamma-induced protein 10 were higher in patients with GATA2 deficiency than in controls. GATA2 is the first gene associated with EBV hydroa vacciniforme-like lymphoma. GATA2 deficiency should be considered in patients with severe primary EBV infection or EBV-associated cancer, especially in those with disseminated nontuberculous mycobacterial disease and warts. Published by Oxford University Press for the Infectious Diseases Society of America 2016. This work is written by (a) US Government employee(s) and is in the public domain in the US.

  17. GATA-4 and FOG-2 expression in pediatric ovarian sex cord-stromal tumors replicates embryonal gonadal phenotype: results from the TREP project.

    Science.gov (United States)

    Virgone, Calogero; Cecchetto, Giovanni; Ferrari, Andrea; Bisogno, Gianni; Donofrio, Vittoria; Boldrini, Renata; Collini, Paola; Dall'Igna, Patrizia; Alaggio, Rita

    2012-01-01

    GATA proteins are a family of zinc finger transcription factors regulating gene expression, differentiation and proliferation in various tissues. The expression of GATA-4 and FOG-2, one of its modulators, was studied in pediatric Sex Cord-Stromal tumors of the ovary, in order to evaluate their potential role as diagnostic markers and prognostic factors. Clinical and histological data of 15 patients, enrolled into the TREP Project since 2000 were evaluated. When available, immunostaines for FOG-2, GATA-4, α-Inhibin, Vimentin and Pancytokeratin were also analyzed. In our series there were 6 Juvenile Granulosa Cell Tumors (JGCT), 6 Sertoli-Leydig Cell Tumors (SLCT), 1 Cellular Fibroma, 1 Theca Cell Tumor and 1 Stromal Sclerosing Tumor (SST). Thirteen patients obtained a complete remission (CR), 1 reached a second CR after the removal of a metachronous tumor and 1 died of disease. Inhibin was detectable in 11/15, Vimentin in 13/15, Pancytokeratin in 6/15, GATA-4 in 5/13 and FOG-2 in 11/15. FOG-2 was highly expressed in 5/6 JGCT, while GATA-4 was weakly detectable only in 1 of the cases. SLCT expressed diffusely FOG-2 (4/6) and GATA-4 (3/5). GATA-4 and FOG-2 were detected in fibroma and thecoma but not in the SST. Pediatric granulosa tumors appear to express a FOG-2/GATA-4 phenotype in keeping with primordial ovarian follicles. High expression of GATA-4 does not correlate with aggressive behaviour as seen in adults, but it is probably involved in cell proliferation its absence can be associated with the better outcome of JGCT. SLCTs replicate the phenotype of Sertoli cells during embryogenesis in normal testis. In this group, the lack of expression of FOG-2 in tumors in advanced stages might reveal a hypothetical role in inhibiting GATA-4 cell proliferation pathway. In fibroma/thecoma group GATA-4 and FOG-2 point out the abnormal activation of GATA pathway and might be involved in the onset of these tumors.

  18. Spectrum of myeloid neoplasms and immune deficiency associated with germline GATA2 mutations

    International Nuclear Information System (INIS)

    Mir, Muhammad A; Kochuparambil, Samith T; Abraham, Roshini S; Rodriguez, Vilmarie; Howard, Matthew; Hsu, Amy P; Jackson, Amie E; Holland, Steven M; Patnaik, Mrinal M

    2015-01-01

    Guanine-adenine-thymine-adenine 2 (GATA2) mutated disorders include the recently described MonoMAC syndrome (Monocytopenia and Mycobacterium avium complex infections), DCML (dendritic cell, monocyte, and lymphocyte deficiency), familial MDS/AML (myelodysplastic syndrome/acute myeloid leukemia) (myeloid neoplasms), congenital neutropenia, congenital lymphedema (Emberger's syndrome), sensorineural deafness, viral warts, and a spectrum of aggressive infections seen across all age groups. While considerable efforts have been made to identify the mutations that characterize this disorder, pathogenesis remains a work in progress with less than 100 patients described in current literature. Varying clinical presentations offer diagnostic challenges. Allogeneic stem cell transplant remains the treatment of choice. Morbidity, mortality, and social costs due to the familial nature of the disease are considerable. We describe our experience with the disorder in three affected families and a comprehensive review of current literature

  19. Sr isotope zoning in plagioclase from andesites at Cabo De Gata, Spai

    DEFF Research Database (Denmark)

    Waight, Tod Earle; Tørnqvist, Jakob

    2018-01-01

    Plagioclase crystals in andesites from the Cabo De Gata region show generally radiogenic Sr isotope compositions and consistent core to rim increases in 87Sr/86Sr that are indicative of open system processes in the lithosphere and crustal contamination during crystallization. High-grade metamorphic...... rocks of the Alpujárride and Nevado-Filábride complexes represent the most likely crustal contaminants. The plagioclases are characterized by subtly zoned and resorbed calcic cores (An73-86). These cores also have radiogenic 87Sr/86Sr (0.7127-0.7129), although less radiogenic than plagioclase rims......, groundmass plagioclase and whole rock compositions (up to 87Sr/86Sr = 0.7135). These cores are interpreted to represent early crystallization of plagioclase from hydrous melts emplaced into the lower crust. The parental melts to these andesites must therefore have already inherited their radiogenic Sr...

  20. Fiscal 1998 regional consortium R and D project (Regional consortium field). Report on R and D of production technology of hybrid-type biocompatible hard tissue replacing materials (1st fiscal year); 1998 nendo chiiki konsoshiamu kenkyu kaihatsu jigyo. Chiiki konsoshiamu bun'ya (hybrid gata seitai yugo kinosei kososhiki daitai sozai seizo gijutsu no kenkyu kaihatsu)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    This project aims at development of high-strength high- biocompatible {beta}-type Ti alloy with lower modulli of elasticity composed of non-toxic elements, phosphate calcium ceramics for improving the biocompatibility and coating technology, and establishment of production technology of hybrid-type biocompatible hard tissue replacing materials. In fiscal 1998, the project promoted the following: Design of high-biocompatible {beta}-type Ti alloy materials, development of thermomechanical treatment for improving dynamic characteristics of such alloy, survey on practical melting and casting technologies and dental precision casting process, evaluation of the biocompatibility of the alloy by cytotoxicity, selection of tools for precision machining and surface finishing, control of contents, orientation and precipitation of biocompatible crystals such as {beta}- Ca(PO{sub 3}){sub 2} fibers for further improvement of the biocompatibility. This paper also outlines the survey results on the market needs, market size and market share for the feasibility of these materials. (NEDO)

  1. FY 2000 report on the results of the regional consortium R and D project - Regional consortium energy R and D. Third year report. R and D of a hybrid pulse plasma coating (HPPC) system; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki consortium energy kenkyu kaihatsu. Hybrid gata pulse plasma coating (HPPC) system no kenkyu kaihatsu (dai 3 nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The development was proceeded with of the surface reforming technology system in which plasma CVD technology and PSII (plasma source ion implantation) technology are connected. HPPC forms DLC (diamond like carbon) layers excellent in adhesion and ceramic layers on the surface of work pieces such as complicatedly shaped dies and tools/sliding parts. Element technologies are the raw material gas pulse introducing technology for efficiently introducing raw materials into the reaction container in a state of gas, high density plasma formation technology and pulse plasma formation technology, minus pulse voltage application technology for vertically implanting various ions on the surface of complicatedly shaped work pieces, and advanced hybrid control technology enabling the control of ion species/implantation amount/layer formation speed. In FY 2000, using the prototype of the HPPC system, studies were made of the development of the DLC layer process and the low temperature DLC layer process, and development and commercialization of technology to improve throwing power, etc. (NEDO)

  2. DAF-16/FOXO and EGL-27/GATA promote developmental growth in response to persistent somatic DNA damage.

    Science.gov (United States)

    Mueller, Michael M; Castells-Roca, Laia; Babu, Vipin; Ermolaeva, Maria A; Müller, Roman-Ulrich; Frommolt, Peter; Williams, Ashley B; Greiss, Sebastian; Schneider, Jennifer I; Benzing, Thomas; Schermer, Bernhard; Schumacher, Björn

    2014-12-01

    Genome maintenance defects cause complex disease phenotypes characterized by developmental failure, cancer susceptibility and premature ageing. It remains poorly understood how DNA damage responses function during organismal development and maintain tissue functionality when DNA damage accumulates with ageing. Here we show that the FOXO transcription factor DAF-16 is activated in response to DNA damage during development, whereas the DNA damage responsiveness of DAF-16 declines with ageing. We find that in contrast to its established role in mediating starvation arrest, DAF-16 alleviates DNA-damage-induced developmental arrest and even in the absence of DNA repair promotes developmental growth and enhances somatic tissue functionality. We demonstrate that the GATA transcription factor EGL-27 co-regulates DAF-16 target genes in response to DNA damage and together with DAF-16 promotes developmental growth. We propose that EGL-27/GATA activity specifies DAF-16-mediated DNA damage responses to enable developmental progression and to prolong tissue functioning when DNA damage persists.

  3. Dew contribution to the water balance in a semiarid coastal steppe ecosystem (Cabo de Gata, SE Spain)

    International Nuclear Information System (INIS)

    Moro, M. J.; Were, A.; Morillas, L.; Villagarcia, L.; Canton, Y.; Lazaro, R.; Serrano-Ortiz, P.; Kowalski, A. S.; Domingo, F.

    2009-01-01

    Dewfall deposition can be a significant source of moisture in arid and semiarid ecosystems, thus contribution to improve daily and annual water balances. Occurrence, frequency and amount of dewfall were measured in the Balsa Blanca site (Cabo de Gata, Almeria, Spain) from January 2007 to May 2008. this area has a sparse vegetation cover dominated by Stipa tenacissima combined with bare soil and biological soil crusts. (Author) 3 refs.

  4. Endoglin expression in blood and endothelium is differentially regulated by modular assembly of the Ets/Gata hemangioblast code.

    Science.gov (United States)

    Pimanda, John E; Chan, Wan Y I; Wilson, Nicola K; Smith, Aileen M; Kinston, Sarah; Knezevic, Kathy; Janes, Mary E; Landry, Josette-Renée; Kolb-Kokocinski, Anja; Frampton, Jonathan; Tannahill, David; Ottersbach, Katrin; Follows, George A; Lacaud, Georges; Kouskoff, Valerie; Göttgens, Berthold

    2008-12-01

    Endoglin is an accessory receptor for TGF-beta signaling and is required for normal hemangioblast, early hematopoietic, and vascular development. We have previously shown that an upstream enhancer, Eng -8, together with the promoter region, mediates robust endothelial expression yet is inactive in blood. To identify hematopoietic regulatory elements, we used array-based methods to determine chromatin accessibility across the entire locus. Subsequent transgenic analysis of candidate elements showed that an endothelial enhancer at Eng +9 when combined with an element at Eng +7 functions as a strong hemato-endothelial enhancer. Chromatin immunoprecipitation (ChIP)-chip analysis demonstrated specific binding of Ets factors to the promoter as well as to the -8, +7+9 enhancers in both blood and endothelial cells. By contrast Pu.1, an Ets factor specific to the blood lineage, and Gata2 binding was only detected in blood. Gata2 was bound only at +7 and GATA motifs were required for hematopoietic activity. This modular assembly of regulators gives blood and endothelial cells the regulatory freedom to independently fine-tune gene expression and emphasizes the role of regulatory divergence in driving functional divergence.

  5. GATA4-mediated cardiac hypertrophy induced by D-myo-inositol 1,4,5-tris-phosphate

    International Nuclear Information System (INIS)

    Zhu Zhiming; Zhu Shanjun; Liu Daoyan; Yu Zengping; Yang Yongjian; Giet, Markus van der; Tepel, Martin

    2005-01-01

    We evaluated the effects of D-myo-inositol 1,4,5-tris-phosphate on cardiac hypertrophy. D-myo-inositol 1,4,5-tris-phosphate augmented cardiac hypertrophy as evidenced by its effects on DNA synthesis, protein synthesis, and expression of immediate-early genes c-myc and c-fos, β-myosin heavy chain, and α-actin. The administration of D-myo-inositol 1,4,5-tris-phosphate increased the expression of nuclear factor of activated T-cells and cardiac-restricted zinc finger transcription factor (GATA4). Real-time quantitative RT-PCR showed that D-myo-inositol 1,4,5-tris-phosphate-induced GATA4 mRNA was significantly enhanced even in the presence of the calcineurin inhibitor, cyclosporine A. The effect of D-myo-inositol 1,4,5-tris-phosphate was blocked after inhibition of inositol-trisphosphate receptors but not after inhibition of c-Raf/mitogen-activated protein kinase kinase (MEK)/mitogen-activated protein kinase (ERK) or p38 mitogen-activated protein kinase pathways. The study shows that D-myo-inositol 1,4,5-tris-phosphate-induced cardiac hypertrophy is mediated by GATA4 but independent from the calcineurin pathway

  6. Seafloor mapping of the southeast Iberian margin (from Cabo de Palos to Cabo de Gata)

    Science.gov (United States)

    Lastras, Galderic; Leon, César; Elvira, Elena; Pascual, Laura; Muñoz, Araceli; de Cárdenas, Enrique; Acosta, Juan; Canals, Miquel

    2014-05-01

    We present the multibeam bathymetry and derived maps of the southeast Iberian margin from Cabo de Palos to Cabo de Gata, 37º35'N to 35º45'N and 2º10'W to 0º20'E, from the coastline down to the Algero-Balearic abyssal plain at depths exceeding 2600 m. The edition of of the maps is carried out within the Complementary Action VALORPLAT ("Scientific valorisation of multibeam bathymetry data from the Spanish continental shelf and slope"), funded by the Spanish Ministry of Economy and Competitivity. The multibeam bathymetry data of the slope and abyssal plain were obtained during different surveys in 2004, 2006 and 2007 on board R/V Vizconde de Eza with a Simrad EM300 multibeam echo-sounder as part of the CAPESME Project, a collaboration between the Spanish Institute of Oceanography (IEO) and General Secretariat of Fisheries (SGP), primarily aiming at creating maps of the fishing grounds of the Mediterranean continental margins of Spain. Multibeam bathymetry data from the continental shelf were obtained within the ESPACE project, also in a cooperative frame between IEO and SGP. The map series is constituted by a general map at 1:400,000 scale and 14 detailed maps at 1:75,000 scale, which include inset maps on slope gradients and seafloor nature (rock or sediment type), the later obtained with rock dredges and Shipeck sediment dredges. Both the detailed maps and the general map are available in paper print, and the whole collection is also distributed in an edited USB. The geological features displayed in the different maps include the continental shelf, with abundant geomorphic features indicative of past sea-level changes, the continental slope carved by the Palos, Tiñoso, Cartagena Este, Cartagena Oeste, Águilas, Almanzora, Alias, Garrucha and Gata submarine canyons, the Mazarrón, Palomares and Al-Mansour escarpments, the Abubácer, Maimonides and Yusuf ridges, the Águilas and Al-Mansour seamounts, and the Algero-Balearic abyssal plain where prominent

  7. High frequency of GATA2 mutations in patients with mild chronic neutropenia evolving to MonoMac syndrome, myelodysplasia, and acute myeloid leukemia.

    Science.gov (United States)

    Pasquet, Marlène; Bellanné-Chantelot, Christine; Tavitian, Suzanne; Prade, Naïs; Beaupain, Blandine; Larochelle, Olivier; Petit, Arnaud; Rohrlich, Pierre; Ferrand, Christophe; Van Den Neste, Eric; Poirel, Hélène A; Lamy, Thierry; Ouachée-Chardin, Marie; Mansat-De Mas, Véronique; Corre, Jill; Récher, Christian; Plat, Geneviève; Bachelerie, Françoise; Donadieu, Jean; Delabesse, Eric

    2013-01-31

    Congenital neutropenia is a group of genetic disorders that involve chronic neutropenia and susceptibility to infections. These neutropenias may be isolated or associated with immunologic defects or extra-hematopoietic manifestations. Complications may occur as infectious diseases, but also less frequently as myelodysplastic syndrome (MDS) or acute myeloid leukemia (AML). Recently, the transcription factor GATA2 has been identified as a new predisposing gene for familial AML/MDS. In the present study, we describe the initial identification by exome sequencing of a GATA2 R396Q mutation in a family with a history of chronic mild neutropenia evolving to AML and/or MDS. The subsequent analysis of the French Severe Chronic Neutropenia Registry allowed the identification of 6 additional pedigrees and 10 patients with 6 different and not previously reportedGATA2 mutations (R204X, E224X, R330X, A372T, M388V, and a complete deletion of the GATA2 locus). The frequent evolution to MDS and AML in these patients reveals the importance of screening GATA2 in chronic neutropenia associated with monocytopenia because of the frequent hematopoietic transformation, variable clinical expression at onset, and the need for aggressive therapy in patients with poor clinical outcome. Mutations of key transcription factor in myeloid malignancies.

  8. Predictive mapping using GIS to locate epithermal gold deposits at Cabo de Gata (Prov. of Almeria, Spain); Cartografia predictiva mediante SIG de depositos epitermales de oro en Cabo de Gata, Almeria, Espana

    Energy Technology Data Exchange (ETDEWEB)

    Rogol-Sanchez, J. P.; Chica-Olmo, M.; Rodriguez-Galiano, V.; Pardo-Iguzquiza, E.

    2011-07-01

    The main aim of mineral potential mapping is to generate predictive maps showing the spatial distribution of a numerical index of favour ability for the presence of a mineral deposit of the type sought. We have studied the mineral favorability for epithermal gold deposits in the Cabo de Gata volcanic field in the Province of Almeria in Spain. Predictive maps deriving from the models suggest the presence of several potentially favourable zones. The performance of predictive maps is similar in most cases. Nevertheless, data-driven methods are able to capture more readily the spatial distribution of known gold occurrences in the area. (Author) 32 refs.

  9. The evolutionary duplication and probable demise of an endodermal GATA factor in Caenorhabditis elegans.

    Science.gov (United States)

    Fukushige, Tetsunari; Goszczynski, Barbara; Tian, Helen; McGhee, James D

    2003-10-01

    We describe the elt-4 gene from the nematode Caenorhabditis elegans. elt-4 is predicted to encode a very small (72 residues, 8.1 kD) GATA-type zinc finger transcription factor. The elt-4 gene is located approximately 5 kb upstream of the C. elegans elt-2 gene, which also encodes a GATA-type transcription factor; the zinc finger DNA-binding domains are highly conserved (24/25 residues) between the two proteins. The elt-2 gene is expressed only in the intestine and is essential for normal intestinal development. This article explores whether elt-4 also has a role in intestinal development. Reporter fusions to the elt-4 promoter or reporter insertions into the elt-4 coding regions show that elt-4 is indeed expressed in the intestine, beginning at the 1.5-fold stage of embryogenesis and continuing into adulthood. elt-4 reporter fusions are also expressed in nine cells of the posterior pharynx. Ectopic expression of elt-4 cDNA within the embryo does not cause detectable ectopic expression of biochemical markers of gut differentiation; furthermore, ectopic elt-4 expression neither inhibits nor enhances the ectopic marker expression caused by ectopic elt-2 expression. A deletion allele of elt-4 was isolated but no obvious phenotype could be detected, either in the gut or elsewhere; brood sizes, hatching efficiencies, and growth rates were indistinguishable from wild type. We found no evidence that elt-4 provided backup functions for elt-2. We used microarray analysis to search for genes that might be differentially expressed between L1 larvae of the elt-4 deletion strain and wild-type worms. Paired hybridizations were repeated seven times, allowing us to conclude, with some confidence, that no candidate target transcript could be identified as significantly up- or downregulated by loss of elt-4 function. In vitro binding experiments could not detect specific binding of ELT-4 protein to candidate binding sites (double-stranded oligonucleotides containing single or multiple

  10. Fungal Morphology, Iron Homeostasis, and Lipid Metabolism Regulated by a GATA Transcription Factor in Blastomyces dermatitidis.

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    Amber J Marty

    2015-06-01

    Full Text Available In response to temperature, Blastomyces dermatitidis converts between yeast and mold forms. Knowledge of the mechanism(s underlying this response to temperature remains limited. In B. dermatitidis, we identified a GATA transcription factor, SREB, important for the transition to mold. Null mutants (SREBΔ fail to fully complete the conversion to mold and cannot properly regulate siderophore biosynthesis. To capture the transcriptional response regulated by SREB early in the phase transition (0-48 hours, gene expression microarrays were used to compare SREB∆ to an isogenic wild type isolate. Analysis of the time course microarray data demonstrated SREB functioned as a transcriptional regulator at 37°C and 22°C. Bioinformatic and biochemical analyses indicated SREB was involved in diverse biological processes including iron homeostasis, biosynthesis of triacylglycerol and ergosterol, and lipid droplet formation. Integration of microarray data, bioinformatics, and chromatin immunoprecipitation identified a subset of genes directly bound and regulated by SREB in vivo in yeast (37°C and during the phase transition to mold (22°C. This included genes involved with siderophore biosynthesis and uptake, iron homeostasis, and genes unrelated to iron assimilation. Functional analysis suggested that lipid droplets were actively metabolized during the phase transition and lipid metabolism may contribute to filamentous growth at 22°C. Chromatin immunoprecipitation, RNA interference, and overexpression analyses suggested that SREB was in a negative regulatory circuit with the bZIP transcription factor encoded by HAPX. Both SREB and HAPX affected morphogenesis at 22°C; however, large changes in transcript abundance by gene deletion for SREB or strong overexpression for HAPX were required to alter the phase transition.

  11. Gonadectomia em gatas impúberes: técnica anestésica

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    Sandra Mastrocinque

    2006-12-01

    Full Text Available O objetivo deste estudo foi descrever e avaliar um protocolo anestésico em filhotes de gatas submetidas a gonadectomia. Foram estudadas 40 fêmeas, com 2 a 4 meses de idade, sem raça definida, encaminhadas para ovariohisterectomia. O protocolo anestésico empregado consistiu de pré-medicação com acepromazina e meperidina e a indução e manutenção anestésicas realizadas com tiletamina-zolazepam. Foram avaliadas freqüências cardíaca e respiratória, saturação periférica da oxihemoglobina, temperatura corpórea, qualidade do relaxamento de coto uterino e pedículos ovarianos, tempo cirúrgico e de recuperação anestésica, bem como qualidade da recuperação anestésica e outros efeitos adversos. Houve redução da freqüência respiratória e manutenção da cardíaca no período trans-operatório. Apenas dois animais necessitaram de suplementação com oxigênio e a qualidade da recuperação foi satisfatoriamente tranqüila. O relaxamento dos pedículos ovarianos e coto uterino foi adequado e não foram observados demais efeitos adversos. Diante dos resultados obtidos pode-se concluir que a técnica anestésica empregada foi segura e efetiva nos animais estudados.

  12. Reciprocal patterns of allergen-induced GATA-3 expression in peripheral blood mononuclear cells from atopics vs. non-atopics.

    Science.gov (United States)

    Macaubas, C; Lee, P T; Smallacombe, T B; Holt, B J; Wee, C; Sly, P D; Holt, P G

    2002-01-01

    T helper (Th)2 cytokines are considered to play a central role in the induction and expression of allergic disease. However, the relative importance of individual cytokines is unclear, and overall disease pathogenesis appears to involve the coordinate activities of a range of Th2 cytokines acting in sequence or in parallel. The present study examines an alternative approach to the study of cytokine gene function in atopy, focusing instead upon T cell transcription factors (TFs) which play a role in the regulation of multiple cytokine genes. To investigate the allergen-induced expression of the TF GATA-3 and c-Maf in peripheral blood mononuclear cells (PBMCs) and in cytokine-driven Th polarization. PBMC from house dust mite (HDM)-atopic and non-atopics were stimulated in vitro with allergen or anti-CD3/IL-2. TF expression was analysed by semiquantitative RT-PCR and major findings were validated by real-time PCR. Cell separations were performed to analyse the contribution of CD45RO+ cells. CD4+ cord blood cells were Th1 or Th2 polarized in vitro by exogenous cytokines and TF expression analysed by Northern blot and real-time PCR. Results We demonstrate for the first time that during differentiation of CD4+ CD45RA+ naïve human T cells towards Th2 commitment, and during allergen-specific reactivation of peripheral CD4+ CD45RO+ Th2 memory cells in established atopics, expression of the Th2-associated TF GATA-3 is rapidly up-regulated, whereas T cells from non-atopics display equally rapid GATA-3 down-regulation under identical conditions of allergen stimulation. These findings identify Th2-associated TFs as key determinants of the atopic phenotype, suggesting their unique potential as therapeutic targets for disease control.

  13. Epigenetic subgroups of esophageal and gastric adenocarcinoma with differential GATA5 DNA methylation associated with clinical and lifestyle factors.

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    Xinhui Wang

    Full Text Available BACKGROUND: Adenocarcinomas located near the gastroesophageal junction have unclear etiology and are difficult to classify. We used DNA methylation analysis to identify subtype-specific markers and new subgroups of gastroesophageal adenocarcinomas, and studied their association with epidemiological risk factors and clinical outcomes. METHODOLOGY/PRINCIPAL FINDINGS: We used logistic regression models and unsupervised hierarchical cluster analysis of 74 DNA methylation markers on 45 tumor samples (44 patients of esophageal and gastric adenocarcinomas obtained from a population-based case-control study to uncover epigenetic markers and cluster groups of gastroesophageal adenocarcinomas. No distinct epigenetic differences were evident between subtypes of gastric and esophageal cancers. However, we identified two gastroesophageal adenocarcinoma subclusters based on DNA methylation profiles. Group membership was best predicted by GATA5 DNA methylation status. We analyzed the associations between these two epigenetic groups and exposure using logistic regression, and the associations with survival time using Cox regression in a larger set of 317 tumor samples (278 patients. There were more males with esophageal and gastric cardia cancers in Cluster Group 1 characterized by higher GATA5 DNA methylation values (all p<0.05. This group also showed associations of borderline statistical significance with having ever smoked (p-value = 0.07, high body mass index (p-value = 0.06, and symptoms of gastroesophageal reflux (p-value = 0.07. Subjects in cluster Group 1 showed better survival than those in Group 2 after adjusting for tumor differentiation grade, but this was not found to be independent of tumor stage. CONCLUSIONS/SIGNIFICANCE: DNA methylation profiling can be used in population-based studies to identify epigenetic subclasses of gastroesophageal adenocarcinomas and class-specific DNA methylation markers that can be linked to

  14. En tránsito a ningún lugar. Sobre Diario de una pasajera de Ágata Gligo

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    Alberto Giordano

    2014-06-01

    This essay presents a reading of Ágata Gligo’s Diario de una pasajera heedful to the ways in which her writing responds to the experiences of unhappiness and frustration related to the autobiographical circumstances that are in their origin: the disease (cancer and the literary block. The present workdiscusses how the act of understanding the disease and its social consequences reduce the diary’s writing performative power great thanksand how the distrust of the language dynamics and the novelist idealization obstruct the diary’s possibility of becoming a literary and existential immanent search.

  15. Regulation of Nitrogen Metabolism by GATA Zinc Finger Transcription Factors in Yarrowia lipolytica

    Energy Technology Data Exchange (ETDEWEB)

    Pomraning, Kyle R.; Bredeweg, Erin L.; Baker, Scott E.

    2017-02-15

    ABSTRACT

    Fungi accumulate lipids in a manner dependent on the quantity and quality of the nitrogen source on which they are growing. In the oleaginous yeastYarrowia lipolytica, growth on a complex source of nitrogen enables rapid growth and limited accumulation of neutral lipids, while growth on a simple nitrogen source promotes lipid accumulation in large lipid droplets. Here we examined the roles of nitrogen catabolite repression and its regulation by GATA zinc finger transcription factors on lipid metabolism inY. lipolytica. Deletion of the GATA transcription factor genesgzf3andgzf2resulted in nitrogen source-specific growth defects and greater accumulation of lipids when the cells were growing on a simple nitrogen source. Deletion ofgzf1, which is most similar to activators of genes repressed by nitrogen catabolite repression in filamentous ascomycetes, did not affect growth on the nitrogen sources tested. We examined gene expression of wild-type and GATA transcription factor mutants on simple and complex nitrogen sources and found that expression of enzymes involved in malate metabolism, beta-oxidation, and ammonia utilization are strongly upregulated on a simple nitrogen source. Deletion ofgzf3results in overexpression of genes with GATAA sites in their promoters, suggesting that it acts as a repressor, whilegzf2is required for expression of ammonia utilization genes but does not grossly affect the transcription level of genes predicted to be controlled by nitrogen catabolite repression. Both GATA transcription factor mutants exhibit decreased expression of genes controlled by carbon catabolite repression via the repressormig1, including genes for beta-oxidation, highlighting the complex interplay between regulation of carbon, nitrogen, and lipid metabolism.

    IMPORTANCENitrogen source is

  16. HER2 and GATA4 are new prognostic factors for early-stage ovarian granulosa cell tumor—a long-term follow-up study

    International Nuclear Information System (INIS)

    Färkkilä, Anniina; Andersson, Noora; Bützow, Ralf; Leminen, Arto; Heikinheimo, Markku; Anttonen, Mikko; Unkila-Kallio, Leila

    2014-01-01

    Granulosa cell tumors (GCTs) carry a risk of recurrence also at an early stage, but reliable prognostic factors are lacking. We assessed clinicopathological prognostic factors and the prognostic roles of the human epidermal growth factor receptors (HER 2–4) and the transcription factor GATA4 in GCTs. We conducted a long-term follow-up study of 80 GCT patients with a mean follow-up time of 16.8 years. A tumor-tissue microarray was immunohistochemically stained for HER2–4 and GATA4. Expression of HER2–4 mRNA was studied by means of real time polymerase chain reaction and HER2 gene amplification was analyzed by means of silver in situ hybridization. The results were correlated to clinical data on recurrences and survival. We found that GCTs have an indolent prognosis, with 5-year disease-specific survival (DSS) being 97.5%. Tumor recurrence was detected in 24% of the patients at a median of 7.0 years (range 2.6–18 years) after diagnosis. Tumor stage was not prognostic of disease-free survival (DFS). Of the molecular prognostic factors, high-level expression of HER2, and GATA4, and high nuclear atypia were prognostic of shorter DFS. In multivariate analyses, high-level coexpression of HER2 and GATA4 independently predicted DFS (hazard ratio [HR] 8.75, 95% CI 2.20–39.48, P = 0.002). High-level expression of GATA4 also predicted shorter DSS (HR 3.96, 95% CI 1.45–12.57, P = 0.006). In multivariate analyses, however, tumor stage (II–III) and nuclear atypia were independent prognostic factors of DSS. In conclusion HER2 and GATA4 are new molecular prognostic markers of GCT recurrence, which could be utilized to optimize the management and follow-up of patients with early-stage GCTs

  17. Senp1 drives hypoxia-induced polycythemia via GATA1 and Bcl-xL in subjects with Monge's disease.

    Science.gov (United States)

    Azad, Priti; Zhao, Huiwen W; Cabrales, Pedro J; Ronen, Roy; Zhou, Dan; Poulsen, Orit; Appenzeller, Otto; Hsiao, Yu Hsin; Bafna, Vineet; Haddad, Gabriel G

    2016-11-14

    In this study, because excessive polycythemia is a predominant trait in some high-altitude dwellers (chronic mountain sickness [CMS] or Monge's disease) but not others living at the same altitude in the Andes, we took advantage of this human experiment of nature and used a combination of induced pluripotent stem cell technology, genomics, and molecular biology in this unique population to understand the molecular basis for hypoxia-induced excessive polycythemia. As compared with sea-level controls and non-CMS subjects who responded to hypoxia by increasing their RBCs modestly or not at all, respectively, CMS cells increased theirs remarkably (up to 60-fold). Although there was a switch from fetal to adult HgbA0 in all populations and a concomitant shift in oxygen binding, we found that CMS cells matured faster and had a higher efficiency and proliferative potential than non-CMS cells. We also established that SENP1 plays a critical role in the differential erythropoietic response of CMS and non-CMS subjects: we can convert the CMS phenotype into that of non-CMS and vice versa by altering SENP1 levels. We also demonstrated that GATA1 is an essential downstream target of SENP1 and that the differential expression and response of GATA1 and Bcl-xL are a key mechanism underlying CMS pathology. © 2016 Azad et al.

  18. Elevated expression of steroidogenesis pathway genes; CYP17, GATA6 and StAR in prenatally androgenized rats.

    Science.gov (United States)

    Jahromi, Marziyeh Salehi; Tehrani, Fahimeh Ramezani; Noroozzadeh, Mahsa; Zarkesh, Maryam; Ghasemi, Asghar; Zadeh-Vakili, Azita

    2016-11-15

    It is believed that excess androgen exposure of the fetus, via altered gene expression, causes hyperandrogenism a key feature of polycystic ovary syndrome (PCOS). The aim of this study was to evaluate expression of Cytochrome P450-17 (CYP17), GATA-binding protein (GAGT6) and Steroidogenic acute regulatory protein (StAR), genes of adult female rats prenatally exposed to androgen excess, closely reflect endocrine and ovarian disturbances of PCOS in women, by comparing them during different phases of estrus cycle with those of non-treated rats. Both the adult prenatally testosterone exposed and control rats (n=23, each) were divided into four groups based on their observed vaginal smear (proestrus, estrus, metestrus and diestrus) and the relative expression of CYP17, GATA6 and StAR genes was measured in ovarian theca cells using Cyber-green Real-Time PCR. Serum sex steroid hormones and gonadotropins levels were measured using the ELISA method; a comparison of these two groups showed that there was an overall increase in the studied genes (CYP17; 2.39 fold change, 95% CI: 1.23-3.55; PPCOS. Copyright © 2016 Elsevier B.V. All rights reserved.

  19. Art27 interacts with GATA4, FOG2 and NKX2.5 and is a novel co-repressor of cardiac genes.

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    Daniel R Carter

    Full Text Available Transcription factors play a crucial role in regulation of cardiac biology. FOG-2 is indispensable in this setting, predominantly functioning through a physical interaction with GATA-4. This study aimed to identify novel co-regulators of FOG-2 to further elaborate on its inhibitory activity on GATA-4. The Art27 transcription factor was identified by a yeast-2-hybrid library screen to be a novel FOG-2 protein partner. Characterisation revealed that Art27 is co-expressed with FOG-2 and GATA-4 throughout cardiac myocyte differentiation and in multiple structures of the adult heart. Art27 physically interacts with GATA-4, FOG-2 and other cardiac transcription factors and by this means, down-regulates their activity on cardiac specific promoters α-myosin heavy chain, atrial natriuretic peptide and B-type natriuretic peptide. Regulation of endogenous cardiac genes by Art27 was shown using microarray analysis of P19CL6-Mlc2v-GFP cardiomyocytes. Together these results suggest that Art27 is a novel transcription factor that is involved in downregulation of cardiac specific genes by physically interacting and inhibiting the activity of crucial transcriptions factors involved in cardiac biology.

  20. Halomonas indalinina sp.nov., a moderately halophilic bacterium isolated from a solar saltern in Cabo de Gata, Al,eria, southern Spain

    NARCIS (Netherlands)

    Cabrera, A.; Aguilera, M.; Fuentes Enriquez de Salamanca, S.; Incerti, C.; Russell, N.J.; Ramos-Cormenzana, A.; Monteoliva-Sanchez, M.

    2007-01-01

    moderately halophilic bacterium, strain CG2.1T, isolated from a solar saltern at Cabo de Gata, a wildlife reserve located in the province of Almería, southern Spain, was subjected to a polyphasic taxonomic study. This organism was an aerobic, motile, Gram-negative rod that produced orange-pigmented

  1. Identification of GATA2 and AP-1 activator elements within the enhancer VNTR occurring in intron 5 of the human SIRT3 gene

    Science.gov (United States)

    Human SIRT3 gene contains an intronic VNTR enhancer. A T > C transition occurring in the second repeat of each VNTR allele implies the presence/absence of a putative GATA binding motif. A partially overlapping AP-1 site, not affected by the transition, was also identified. Aims of the present study ...

  2. The solution structure of the N-terminal zinc finger of GATA-1 reveals a specific binding face for the transcriptional co-factor FOG

    International Nuclear Information System (INIS)

    Kowalski, K.; Czolij, R.; King, G.F.; Crossley, M.; Mackay, J.P.

    1999-01-01

    Zinc fingers (ZnFs) are generally regarded as DNA-binding motifs. However, a number of recent reports have implicated particular ZnFs in the mediation of protein-protein interactions. The N-terminal ZnF of GATA-1 (NF) is one such finger, having been shown to interact with a number of other proteins, including the recently discovered transcriptional co-factor FOG. Here we solve the three-dimensional structure of the NF in solution using multidimensional 1H/15N NMR spectroscopy, and we use 1H/15N spin relaxation measurements to investigate its backbone dynamics. The structure consists of two distorted β-hairpins and a single α-helix, and is similar to that of the C-terminal ZnF of chicken GATA-1. Comparisons of the NF structure with those of other C4-type zinc binding motifs, including hormone receptor and LIM domains, also reveal substantial structural homology. Finally, we use the structure to map the spatial locations of NF residues shown by mutagenesis to be essential for FOG binding, and demonstrate that these residues all lie on a single face of the NF. Notably, this face is well removed from the putative DNA- binding face of the NF, an observation which is suggestive of simultaneous roles for the NF; that is, stabilisation of GATA-1 DNA complexes and recruitment of FOG to GATA-1-controlled promoter regions

  3. Expression Analysis of Gata4, Tbx5 and Nkx2.5 Genes Involved in Congenital Heart Disease

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    Mahta Mazaheri-Naeeini

    2016-04-01

    Full Text Available Background Congenital heart disease (CHD is the most widespread congenital disease in newborn babies and is one of the main causes of death worldwide. The causal agent of heart congenital diseases is unknown but genetic factors have an important role in prevalence of disease. Objectives The main objective of this research is comparison of the gene expression level of three Gata4, Tbx5 and Nkx2.5 genes in three groups of children between 6 months and 13 year old with congenital heart disease. Patients and Methods In this case-control study, 30 samples from each cyanotic and acyanotic patients and 30 samples from healthy children as control were used. RNA extraction was done using commercial kit and gene expression analysis was performed by qRT-PCR approach in three replication using Gata4, Tbx5 and Nkx2.5 genes. Data analysis was done by REST software. Results The results of RNA extraction and cDNA synthesis of all sample showed high quantity and quality of genetic materials. Expression level of tested genes was reduced in two patients group. In cyanotic group reduction was more than acyanotic samples. All tested gene were reduced in both group. Tbx5 gene was suppressed more than other genes. Conclusions Based on our results we could conclude that a gene family play an important role in cardiogenesis process and heart formation. These genes are closely related together. So a genetic consultation for such diseases on parents of these patients to determine the probable genetic mutations is recommended.

  4. Nicotine promotes initiation and progression of KRAS-induced pancreatic cancer via Gata6-dependent dedifferentiation of acinar cells in mice.

    Science.gov (United States)

    Hermann, Patrick C; Sancho, Patricia; Cañamero, Marta; Martinelli, Paola; Madriles, Francesc; Michl, Patrick; Gress, Thomas; de Pascual, Ricardo; Gandia, Luis; Guerra, Carmen; Barbacid, Mariano; Wagner, Martin; Vieira, Catarina R; Aicher, Alexandra; Real, Francisco X; Sainz, Bruno; Heeschen, Christopher

    2014-11-01

    Although smoking is a leading risk factor for pancreatic ductal adenocarcinoma (PDAC), little is known about the mechanisms by which smoking promotes initiation or progression of PDAC. We studied the effects of nicotine administration on pancreatic cancer development in Kras(+/LSLG12Vgeo);Elas-tTA/tetO-Cre (Ela-KRAS) mice, Kras(+/LSLG12D);Trp53+/LSLR172H;Pdx-1-Cre (KPC) mice (which express constitutively active forms of KRAS), and C57/B6 mice. Mice were given nicotine for up to 86 weeks to produce blood levels comparable with those of intermediate smokers. Pancreatic tissues were collected and analyzed by immunohistochemistry and reverse transcriptase polymerase chain reaction; cells were isolated and assayed for colony and sphere formation and gene expression. The effects of nicotine were also evaluated in primary pancreatic acinar cells isolated from wild-type, nAChR7a(-/-), Trp53(-/-), and Gata6(-/-);Trp53(-/-) mice. We also analyzed primary PDAC cells that overexpressed GATA6 from lentiviral expression vectors. Administration of nicotine accelerated transformation of pancreatic cells and tumor formation in Ela-KRAS and KPC mice. Nicotine induced dedifferentiation of acinar cells by activating AKT-ERK-MYC signaling; this led to inhibition of Gata6 promoter activity, loss of GATA6 protein, and subsequent loss of acinar differentiation and hyperactivation of oncogenic KRAS. Nicotine also promoted aggressiveness of established tumors as well as the epithelial-mesenchymal transition, increasing numbers of circulating cancer cells and their dissemination to the liver, compared with mice not exposed to nicotine. Nicotine induced pancreatic cells to acquire gene expression patterns and functional characteristics of cancer stem cells. These effects were markedly attenuated in K-Ras(+/LSL-G12D);Trp53(+/LSLR172H);Pdx-1-Cre mice given metformin. Metformin prevented nicotine-induced pancreatic carcinogenesis and tumor growth by up-regulating GATA6 and promoting

  5. [Effect of Acupuncture on the Expression of Transcription Factor T-bet/GATA-3 in Plasma of Rats with Chronic Fatigue Syndrome].

    Science.gov (United States)

    Wang, Xiang-Yi; Liu, Chang-Zheng; Lei, Bo

    2017-06-25

    To observe the effect of acupuncture on the expression of T-box expressed in T cell (T-bet)/GATA binding factor-3 (GATA-3) in plasma of rats with chronic fatigue syndrome (CFS) and explore the mechanism of acupuncture treatment for CFS. Forty-eight healthy male SD rats were randomly divided into blank control group, CFS model group, acupuncture group, and ginsenoside group (12 rats in each group). CFS rat model was established by combining restriction and cold water swimming. Acupuncture was applied to "Baihui"(GV 20), "Guanyuan" (CV 4) and "Zusanli" (ST 36, bilate-ral) acupoints, once a day for two weeks. The ginsenoside group was gavage administrated with ginsenoside, once a day for two weeks. After 14 days, behavioural changes were observed, and the expression levels of T-bet/GATA-3 genes in plasma were detected by RT-PCR. Compared with the blank control group, the time for immobility of forced suspensory test was signi-ficantly longer ( P <0.05) and the time for exhaustive swimming was significantly shortened ( P <0.05) in the CFS model group. Compared with the model group, the two indexes above-mentioned were reversed ( P <0.05) both in the acupuncture group and the ginsenoside group, and the effects in the acupuncture group were more significant than those in the ginsenoside group ( P <0.05). Compared with the blank control group, the expression level of T-cell transcription factor T-bet gene in plasma was higher in the CFS model group ( P <0.05), companied with lower GATA-3 gene expression ( P <0.05). The ratio of T-bet/GATA-3 was higher in the model group than in the blank control group( P <0.05). Compared with the CFS model group, all the indexes above-mentioned were reversed ( P <0.05) in the two treatment groups. Acupuncture group showed a better effect on reducing T-bet gene expression than the ginsenoside group ( P <0.05). Acupuncture can decrease the expression level of T-bet gene while increase the expression of GATA-3 gene, which may be associated

  6. Symbolic Representation and Protection of the Marine Landscape in the Natural Park of Cabo de Gata-Níjar (Almería- Spain

    Directory of Open Access Journals (Sweden)

    Carmen Gaona Pisonero

    2017-09-01

    Full Text Available This case study article centered on the Natural Park of Cabo de Gata-Níjar (Almería-España on the correspondence or mismatch between the virtual landscape representations and the socio-cultural reality of the protected natural space. Therefore, the purpose of this article is to analyze the reconstruction of the natural market of Cabo de Gata Níjar in some sectors of the Spanish public sphere. The reflection focuses on the visibility of the virtual symbolic representative plains of space. The analysis of the results detects the virtual emic representations a reframing of the Park characterized by an on-line spectacular landscape. A dreamy exaltation in the emic representations virtual which practically invisibilizan real problems attacking against the coasts and reserve marine of this area. On the contrary, the media representation reflects with greater correspondence the dialectical reality of the landscape.

  7. PEER REVIEW (Correlation between GATA-3, Ki67 and p53 expressions to Histopathology grading of Breast Cancer bin Makassar, Indonesia)

    OpenAIRE

    Islam, Andi Asadul

    2016-01-01

    - PEER REVIEW Judul karya Ilmiah (artikel): Correlation between GATA-3, Ki67 and p53 expressions to Histopathology grading of Breast Cancer bin Makassar, Indonesia : 9 Orang : Penulis Ketiga : a. Nama Jurnal : Cancer Research Journal b. Nomor ISSN : 2330-8192, E: 2330-8214 c. Volume,nomor, bulan, tahun : Volume 4, No. 3 Tahun 2016 d. Penerbit : Science PG e. DOI artikel (Jika ada) : 10.11648/j.crj .20160403.11 f. Alamat web Jurnal : www.sciencepublishingg...

  8. Picroside II Attenuates Airway Inflammation by Downregulating the Transcription Factor GATA3 and Th2-Related Cytokines in a Mouse Model of HDM-Induced Allergic Asthma.

    Directory of Open Access Journals (Sweden)

    Jin Choi

    Full Text Available Picroside II isolated from Pseudolysimachion rotundum var. subintegrum has been used as traditional medicine to treat inflammatory diseases. In this study, we assessed whether picroside II has inhibitory effects on airway inflammation in a mouse model of house dust mite (HDM-induced asthma. In the HDM-induced asthmatic model, picroside II significantly reduced inflammatory cell counts in the bronchoalveolar lavage fluid (BALF, the levels of total immunoglobulin (Ig E and HDM-specific IgE and IgG1 in serum, airway inflammation, and mucus hypersecretion in the lung tissues. ELISA analysis showed that picroside II down-regulated the levels of Th2-related cytokines (including IL-4, IL-5, and IL-13 and asthma-related mediators, but it up-regulated Th1-related cytokine, IFNγ in BALF. Picroside II also inhibited the expression of Th2 type cytokine genes and the transcription factor GATA3 in the lung tissues of HDM-induced mice. Finally, we demonstrated that picroside II significantly decreased the expression of GATA3 and Th2 cytokines in developing Th2 cells, consistent with in vivo results. Taken together, these results indicate that picroside II has protective effects on allergic asthma by reducing GATA3 expression and Th2 cytokine bias.

  9. Effects of Shen-Fu Injection on the Expression of T-Cell-Specific Transcription Factors T-bet/Gata-3 in Porcine Postresuscitation Lung Injury

    Directory of Open Access Journals (Sweden)

    Wei Gu

    2013-01-01

    Full Text Available Shen-Fu injection (SFI derived from the ancient traditional Chinese medicine. In this study, the effects of SFI on the expression of T-bet/GATA-3 and its potential mechanisms causing the shift of T cells from Th2 to Th1 on postresuscitation lung injury were examined in a porcine model of cardiac arrest. 30 pigs were randomly divided into SHAM ( and three return of spontaneous circulation (ROSC groups ( per group; 24 pigs were subjected to 8 min of electrically induced cardiac arrest and 2 min of basic life support, which received central venous injection of Shen-Fu (SFI, epinephrine (EP or saline (SA. After successful ROSC, 18 surviving pigs were sacrificed at 24 h after ROSC ( per group. The levels of serum and lung tissue interleukin (IL-4 and interferon (IFN-γ were measured by ELISA, and the protein and mRNA levels of GATA-3 and T-bet in the lung tissue were determined by western blotting and quantitative real-time polymerase chain reaction, respectively. Compared with the EP and SA groups, SFI treatment reduced the levels of IL-4 (, increased levels of IFN-γ (, and induced T-bet mRNA upregulation and GATA-3 mRNA downregulation (. SFI attenuated lung injury and regulated lung immune disorders. Therefore, SFI could protect postresuscitation lung injury by modulating a Th1/Th2 imbalance.

  10. Biodistribution of the GATA-3-specific DNAzyme hgd40 after inhalative exposure in mice, rats and dogs

    International Nuclear Information System (INIS)

    Turowska, Agnieszka; Librizzi, Damiano; Baumgartl, Nadja; Kuhlmann, Jens; Dicke, Tanja; Merkel, Olivia; Homburg, Ursula; Höffken, Helmut; Renz, Harald; Garn, Holger

    2013-01-01

    The DNAzyme hgd40 was shown to effectively reduce expression of the transcription factor GATA-3 RNA which plays an important role in the regulation of Th2-mediated immune mechanisms such as in allergic bronchial asthma. However, uptake, biodistribution and pharmacokinetics of hgd40 have not been investigated yet. We examined local and systemic distribution of hgd40 in naive mice and mice suffering from experimental asthma. Furthermore, we evaluated the pharmacokinetics as a function of dose following single and repeated administration in rats and dogs. Using intranasal administration of fluorescently labeled hgd40 we demonstrated that the DNAzyme was evenly distributed in inflamed asthmatic mouse lungs within minutes after single dose application. Systemic distribution was investigated in mice using radioactive labeled hgd40. After intratracheal application, highest amounts of hgd40 were detected in the lungs. High amounts were also detected in the bladder indicating urinary excretion as a major elimination pathway. In serum, low systemic hgd40 levels were detected already at 5 min post application (p.a.), subsequently decreasing over time to non-detectable levels at 2 h p.a. As revealed by Single Photon Emission Computed Tomography, trace amounts of hgd40 were detectable in lungs up to 7 days p.a. Also in the toxicologically relevant rats and dogs, hgd40 was detectable in blood only shortly after inhalative application. The plasma pharmacokinetic profile was dose and time dependent. Repeated administration did not lead to drug accumulation in plasma of dogs and rats. These pharmacokinetic of hgd40 provide guidance for clinical development, and support an infrequent and convenient dose administration regimen. - Highlights: • Local and systemic distribution of GATA-3-specific DNAzyme hgd40 was investigated. • Pharmacokinetics of hgd40 was tested in rats and dogs. • hgd40 dissolved in PBS was easily taken up into the lungs after local application. • No

  11. Biodistribution of the GATA-3-specific DNAzyme hgd40 after inhalative exposure in mice, rats and dogs

    Energy Technology Data Exchange (ETDEWEB)

    Turowska, Agnieszka [sterna biologicals GmbH and Co. KG, Marburg (Germany); Librizzi, Damiano [Department of Nuclear Medicine, University Hospital Giessen and Marburg GmbH, Baldingerstrasse, 35043 Marburg (Germany); Baumgartl, Nadja [Institute of Laboratory Medicine and Pathobiochemistry-Molecular Diagnostics, Philipps University of Marburg (Germany); Kuhlmann, Jens; Dicke, Tanja [sterna biologicals GmbH and Co. KG, Marburg (Germany); Merkel, Olivia [Department of Pharmaceutical Sciences, Wayne State University, Detroit (United States); Homburg, Ursula [sterna biologicals GmbH and Co. KG, Marburg (Germany); Höffken, Helmut [Department of Nuclear Medicine, University Hospital Giessen and Marburg GmbH, Baldingerstrasse, 35043 Marburg (Germany); Renz, Harald [Institute of Laboratory Medicine and Pathobiochemistry-Molecular Diagnostics, Philipps University of Marburg (Germany); Garn, Holger, E-mail: garn@staff.uni-marburg.de [Institute of Laboratory Medicine and Pathobiochemistry-Molecular Diagnostics, Philipps University of Marburg (Germany)

    2013-10-15

    The DNAzyme hgd40 was shown to effectively reduce expression of the transcription factor GATA-3 RNA which plays an important role in the regulation of Th2-mediated immune mechanisms such as in allergic bronchial asthma. However, uptake, biodistribution and pharmacokinetics of hgd40 have not been investigated yet. We examined local and systemic distribution of hgd40 in naive mice and mice suffering from experimental asthma. Furthermore, we evaluated the pharmacokinetics as a function of dose following single and repeated administration in rats and dogs. Using intranasal administration of fluorescently labeled hgd40 we demonstrated that the DNAzyme was evenly distributed in inflamed asthmatic mouse lungs within minutes after single dose application. Systemic distribution was investigated in mice using radioactive labeled hgd40. After intratracheal application, highest amounts of hgd40 were detected in the lungs. High amounts were also detected in the bladder indicating urinary excretion as a major elimination pathway. In serum, low systemic hgd40 levels were detected already at 5 min post application (p.a.), subsequently decreasing over time to non-detectable levels at 2 h p.a. As revealed by Single Photon Emission Computed Tomography, trace amounts of hgd40 were detectable in lungs up to 7 days p.a. Also in the toxicologically relevant rats and dogs, hgd40 was detectable in blood only shortly after inhalative application. The plasma pharmacokinetic profile was dose and time dependent. Repeated administration did not lead to drug accumulation in plasma of dogs and rats. These pharmacokinetic of hgd40 provide guidance for clinical development, and support an infrequent and convenient dose administration regimen. - Highlights: • Local and systemic distribution of GATA-3-specific DNAzyme hgd40 was investigated. • Pharmacokinetics of hgd40 was tested in rats and dogs. • hgd40 dissolved in PBS was easily taken up into the lungs after local application. • No

  12. FY 2000 report on the results of the regional consortium R and D project - Regional consortium energy field. Second year report. Development of the energy saving manufacturing process of smart materials having electromagnetic wave absorbing function using the microwave-hydrothermal method; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki contortium energy bun'ya. Micro ha - suinetsuho wo riyoshita denjiha kyushu kino wo yusuru smart zairyo no sho energy gata seizo process no kaihatsu (dai 2 nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The development was proceeded with of electromagnetic wave absorbing materials (board) which dispersed carbon fiber as conducting material and ferrite as magnetic material to matrices such as resin and cement. With the multi-layer structure as a basis, the material has wave absorbing ability in the area of 300MHz-60GHz band. The material is presumed to be applied to wall construction use materials and bodies of electronic equipment since it prevents the radio wave reflection caused by structures such as bridges. Ferrite was synthesized by microwave-hydrothermal method (500kPa, 2.54GHz). Further, carbon fiber was covered with ferrite for improvement of absorption characteristics. Studies were made in the following 5 fields: 1) design of smart materials and development of hybrid process technology; 2) study on the evaluation of wave absorbing function; 3) R and D of the manufacturing process of smart forming materials; 4) development of the fiber surface processing process using ocean resource; 5) comprehensive investigational study. In 1), study was conducted on relations among electromagnetic shielding characteristics of the ferrite-covering carbon fiber, fiber length and fiber content. (NEDO)

  13. FY 2000 report on the results of the regional consortium R and D project - Regional consortium field. Third year report. R and D of the manufacturing technology of hybrid type biocompatible hard tissue substituting materials; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki consortium bun'ya. Hybrid gata seitai yugo kinosei kososhiki daitai sozai seizo gijutsu no kenkyu kaihatsu (dai 3 nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    As instrument materials substituting for hard tissue such as tooth root and artificial hip joint, the technology development is being proceeded with of the use of high-biocompatible {beta} Ti alloys coated by hydroxy apatite. Studies were made in the following 6 fields: 1) design of high-biocompatible {beta} Ti alloys, system control and dynamic evaluation; 2) development of high efficiency calcium phosphate ceramics (hydroxy apatite) and development of surface coating technology; 3) development of melting casting technology of high-biocompatible {beta} Ti alloys; 4) biological evaluation of biocompatibility of high-biocompatible {beta} Ti alloys and completion of dental precision casting technology; 5) basic research on affinity of low rigidity Ti alloys (trial manufacture); 6) development of technology of precision processing and precision finishing processing of alloys. In 5), in the test on affinity of the trial-manufactured alloys, stainless steel and existing Ti alloys, it was verified that the trial-manufactured alloys were excellent in affinity. (NEDO)

  14. The nT1 translocation separates vulval regulatory elements from the egl-18 and elt-6 GATA factor genes.

    Science.gov (United States)

    Koh, Kyunghee; Bernstein, Yelena; Sundaram, Meera V

    2004-03-01

    egl-18 and elt-6 are partially redundant, adjacent genes encoding GATA factors essential for viability, seam cell development, and vulval development in Caenorhabditis elegans. The nT1 reciprocal translocation causes a strong Vulvaless phenotype, and an nT1 breakpoint was previously mapped to the left arm of LGIV, where egl-18/elt-6 are located. Here we present evidence that the nT1 vulval phenotype is due to a disruption of egl-18/elt-6 function specifically in the vulva. egl-18 mutations do not complement nT1 for vulval defects, and the nT1 breakpoint on LGIV is located within approximately 800 bp upstream of a potential transcriptional start site of egl-18. In addition, we have identified a approximately 350-bp cis-regulatory region sufficient for vulval expression just upstream of the nT1 breakpoint. By examining the fusion state and division patterns of the cells in the developing vulva of nT1 mutants, we demonstrate that egl-18/elt-6 prevent fusion and promote cell proliferation at multiple steps of vulval development.

  15. Sr isotope zoning in plagioclase from andesites at Cabo De Gata, Spain: Evidence for shallow and deep contamination

    Science.gov (United States)

    Waight, Tod E.; Tørnqvist, Jakob B.

    2018-05-01

    Plagioclase crystals in andesites from the Cabo De Gata region show generally radiogenic Sr isotope compositions and consistent core to rim increases in 87Sr/86Sr that are indicative of open system processes in the lithosphere and crustal contamination during crystallization. High-grade metamorphic rocks of the Alpujárride and Nevado-Filábride complexes represent the most likely crustal contaminants. The plagioclases are characterized by subtly zoned and resorbed calcic cores (An73-86). These cores also have radiogenic 87Sr/86Sr (0.7127-0.7129), although typically less radiogenic than plagioclase rims, groundmass plagioclase and whole rock compositions (up to 87Sr/86Sr = 0.7135). These cores are interpreted to represent early crystallization of plagioclase from hydrous melts emplaced into the lower crust. The parental melts to these andesites must therefore have already inherited their radiogenic Sr isotope compositions prior to entering the lower crust and before the onset of crystallization of plagioclase, which is inconsistent with previous models suggesting that the generally radiogenic nature of Sr in these volcanics reflects large amounts of crustal contamination. Instead, the isotope systematics are consistent with models invoked significant addition of a subducted sediment component to the mantle source. The high-An% plagioclase cores are characterized by resorption textures, which are consistent with dissolution during rapid decompression and/or devolatisation during magma migration from the lower crust into upper crustal magma chambers.

  16. Constraining controls on carbonate sequences with high-resolution chronostratigraphy: Upper Miocene, Cabo de Gata region, SE Spain

    Science.gov (United States)

    Montgomery, P.; Farr, M.R.; Franseen, E.K.; Goldstein, R.H.

    2001-01-01

    A high-resolution chronostratigraphy has been developed for Miocene shallow-water carbonate strata in the Cabo de Gata region of SE Spain for evaluation of local, regional and global factors that controlled platform architecture prior to and during the Messinian salinity crisis. Paleomagnetic data were collected from strata at three localities. Mean natural remanent magnetization (NRM) ranges between 1.53 ?? 10-8 and 5.2 ?? 10-3 Am2/kg. Incremental thermal and alternating field demagnetization isolated the characteristic remanent magnetization (ChRM). Rock magnetic studies show that the dominant magnetic mineral is magnetite, but mixtures of magnetite and hematite occur. A composite chronostratigraphy was derived from five stratigraphic sections. Regional stratigraphic data, biostratigraphic data, and an 40Ar/39Ar date of 8.5 ?? 0.1 Ma, for an interbedded volcanic flow, place the strata in geomagnetic polarity Chrons C4r to C3r. Sequence-stratigraphic and diagenetic evidence indicate a major unconformity at the base of depositional sequence (DS)3 that contains a prograding reef complex, suggesting that approximately 250 000 yr of record (Subchrons C3Br.2r to 3Br.1r) are missing near the Messinian-Tortonian boundary. Correlation to the GPTS shows that the studied strata represent five third- to fourth-order DSs. Basal units are temperate to subtropical ramps (DS1A, DS1B, DS2); these are overlain by subtropical to tropical reefal platforms (DS3), which are capped by subtropical to tropical cyclic carbonates (Terminal Carbonate Complex, TCC). Correlation of the Cabo de Gata record to the Melilla area of Morocco, and the Sorbas basin of Spain indicate that early - Late Tortonian ramp strata from these areas are partially time-equivalent. Similar strata are extensively developed in the Western Mediterranean and likely were influenced by a cool climate or influx of nutrients during an overall rise in global sea-level. After ramp deposition, a sequence boundary (SB3) in

  17. UTERINE PROLAPSE IN QUEEN AND UTERINE PROLAPSO UTERINO EM GATA E RETROFLEXÃO UTERINA EM CADELA

    Directory of Open Access Journals (Sweden)

    Giuliano Queiroz Mostachio

    2008-10-01

    Full Text Available

    Obstetrical emergencies are problem in veterinary clinics and hospital. So, the aim of this report is to describe the clinical-surgical aspect of one of them, the uterine prolapse. Complete protrusion and retroflexion of uterus had been diagnosed in a queen and female dog, respectively. After the stabilization of the animals and reduction of the prolapses followed by ovary-hysterectomy, one of the animals came to death due to septicemia and hypovolemic shock. Rapid assessment and intensive treatments are required to sustain the life of the animal.

    KEY WORDS: Cat, dog, uterine prolapse, uterine retroflexion.

    Emergências obstétricas, como o prolapso uterino, constituem um problema em clínicas e hospitais veterinários. Neste relato descreve-se o aspecto clínico-cirúrgico do prolapso uterino. Diagnosticaram-se, em uma gata e em uma cadela, protrusão completa e retroflexão uterina, respectivamente. Após a estabilização dos animais e redução dos prolapsos seguidos de ovário-histerectomia, um dos animais veio a óbito, em decorrência de septicemia e choque hipovolêmico. Portanto, a rápida avaliação e tratamentos intensivos são requeridos para assegurar a vida do animal.

     

    PALAVRAS-CHAVES: Cão, gato, prolapso uterino, retroflexão uterina

  18. GATA-Dependent Glutaminolysis Drives Appressorium Formation in Magnaporthe oryzae by Suppressing TOR Inhibition of cAMP/PKA Signaling.

    Science.gov (United States)

    Marroquin-Guzman, Margarita; Wilson, Richard A

    2015-04-01

    Fungal plant pathogens are persistent and global food security threats. To invade their hosts they often form highly specialized infection structures, known as appressoria. The cAMP/ PKA- and MAP kinase-signaling cascades have been functionally delineated as positive-acting pathways required for appressorium development. Negative-acting regulatory pathways that block appressorial development are not known. Here, we present the first detailed evidence that the conserved Target of Rapamycin (TOR) signaling pathway is a powerful inhibitor of appressorium formation by the rice blast fungus Magnaporthe oryzae. We determined TOR signaling was activated in an M. oryzae mutant strain lacking a functional copy of the GATA transcription factor-encoding gene ASD4. Δasd4 mutant strains could not form appressoria and expressed GLN1, a glutamine synthetase-encoding orthologue silenced in wild type. Inappropriate expression of GLN1 increased the intracellular steady-state levels of glutamine in Δasd4 mutant strains during axenic growth when compared to wild type. Deleting GLN1 lowered glutamine levels and promoted appressorium formation by Δasd4 strains. Furthermore, glutamine is an agonist of TOR. Treating Δasd4 mutant strains with the specific TOR kinase inhibitor rapamycin restored appressorium development. Rapamycin was also shown to induce appressorium formation by wild type and Δcpka mutant strains on non-inductive hydrophilic surfaces but had no effect on the MAP kinase mutant Δpmk1. When taken together, we implicate Asd4 in regulating intracellular glutamine levels in order to modulate TOR inhibition of appressorium formation downstream of cPKA. This study thus provides novel insight into the metabolic mechanisms that underpin the highly regulated process of appressorium development.

  19. GATA-Dependent Glutaminolysis Drives Appressorium Formation in Magnaporthe oryzae by Suppressing TOR Inhibition of cAMP/PKA Signaling.

    Directory of Open Access Journals (Sweden)

    Margarita Marroquin-Guzman

    2015-04-01

    Full Text Available Fungal plant pathogens are persistent and global food security threats. To invade their hosts they often form highly specialized infection structures, known as appressoria. The cAMP/ PKA- and MAP kinase-signaling cascades have been functionally delineated as positive-acting pathways required for appressorium development. Negative-acting regulatory pathways that block appressorial development are not known. Here, we present the first detailed evidence that the conserved Target of Rapamycin (TOR signaling pathway is a powerful inhibitor of appressorium formation by the rice blast fungus Magnaporthe oryzae. We determined TOR signaling was activated in an M. oryzae mutant strain lacking a functional copy of the GATA transcription factor-encoding gene ASD4. Δasd4 mutant strains could not form appressoria and expressed GLN1, a glutamine synthetase-encoding orthologue silenced in wild type. Inappropriate expression of GLN1 increased the intracellular steady-state levels of glutamine in Δasd4 mutant strains during axenic growth when compared to wild type. Deleting GLN1 lowered glutamine levels and promoted appressorium formation by Δasd4 strains. Furthermore, glutamine is an agonist of TOR. Treating Δasd4 mutant strains with the specific TOR kinase inhibitor rapamycin restored appressorium development. Rapamycin was also shown to induce appressorium formation by wild type and Δcpka mutant strains on non-inductive hydrophilic surfaces but had no effect on the MAP kinase mutant Δpmk1. When taken together, we implicate Asd4 in regulating intracellular glutamine levels in order to modulate TOR inhibition of appressorium formation downstream of cPKA. This study thus provides novel insight into the metabolic mechanisms that underpin the highly regulated process of appressorium development.

  20. Effector/memory CD4 T cells making either Th1 or Th2 cytokines commonly co-express T-bet and GATA-3.

    Directory of Open Access Journals (Sweden)

    Arundhoti Das

    Full Text Available Naïve CD4 T (NCD4T cells post-activation undergo programming for inducible production of cytokines leading to generation of memory cells with various functions. Based on cytokine based polarization of NCD4T cells in vitro, programming for either 'Th1' (interferon-gamma [IFNg] or 'Th2' (interleukin [IL]-4/5/13 cytokines is thought to occur via mutually exclusive expression and functioning of T-bet or GATA-3 transcription factors (TFs. However, we show that a high proportion of mouse and human memory-phenotype CD4 T (MCD4T cells generated in vivo which expressed either Th1 or Th2 cytokines commonly co-expressed T-bet and GATA-3. While T-bet levels did not differ between IFNg-expressing and IL-4/5/13-expressing MCD4T cells, GATA-3 levels were higher in the latter. These observations were also confirmed in MCD4T cells from FVB/NJ or aged C57BL/6 or IFNg-deficient mice. While MCD4T cells from these strains showed greater Th2 commitment than those from young C57BL/6 mice, pattern of co-expression of TF was similar. Effector T cells generated in vivo following immunization also showed TF co-expression in Th1 or Th2 cytokine producing cells. We speculated that the difference in TF expression pattern of MCD4T cells generated in vivo and those generated in cytokine polarized cultures in vitro could be due to relative absence of polarizing conditions during activation in vivo. We tested this by NCD4T cell activation in non-polarizing conditions in vitro. Anti-CD3 and anti-CD28-mediated priming of polyclonal NCD4T cells in vitro without polarizing milieu generated cells that expressed either IFNg or IL-4/5/13 but not both, yet both IFNg- and IL-4/5/13-expressing cells showed upregulation of both TFs. We also tested monoclonal T cell populations activated in non-polarizing conditions. TCR-transgenic NCD4T cells primed in vitro by cognate peptide in non-polarizing conditions which expressed either IFNg or IL-4/5/13 also showed a high proportion of cells co

  1. Braçadeira de poliamida e fio de náilon na ovário-histerectomia em gatas

    OpenAIRE

    PRADO, Tales Dias do

    2012-01-01

    A ovário-histerectomia representa um dos procedimentos mais realizados pela clínica de pequenos animais, tendo por finalidade o controle da natalidade, prevenção de cio e pseudogestação, como também, tratar afecções do trato reprodutor feminino. Foram descritas muitas variações de técnicas de ovário-histerectomia, incluindo, além da tradicional abordagem pela linha média, abordagem pelo flanco e diversas abordagens laparoscópicas. Participaram, no presente estudo, 24 gatas hígidas, sem raça d...

  2. Importância do binômio design e engenharia no beneficiamento de rejeito mineral de opala e ágata na produção de camafeus por usinagem CNC

    OpenAIRE

    Camila Sieburger Tessmann

    2009-01-01

    No estado do Rio Grande do Sul, os garimpos de pedras preciosas estão distribuídos em duas regiões principais: no Distrito Mineiro de Ametista do Sul (maior produtor de ametista) e no Distrito Mineiro de Salto do Jacuí (maior produtor de ágata). Nestes dois distritos mineiros são explorados geodos, parcial ou totalmente preenchidos por camadas intercaladas de ágata, quartzo incolor, ametista, calcita e opala. O processo de extração desses geodos gera grande quantidade de rejeito, composto tan...

  3. A genetic variant near GATA3 implicated in inherited susceptibility and etiology of benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS).

    Science.gov (United States)

    Na, Rong; Helfand, Brian T; Chen, Haitao; Conran, Carly A; Crawford, Susan E; Hayward, Simon W; Tammela, Teuvo L J; Hoffman-Bolton, Judy; Zheng, Siqun L; Walsh, Patrick C; Schleutker, Johanna; Platz, Elizabeth A; Isaacs, William B; Xu, Jianfeng

    2017-08-01

    Benign prostatic hyperplasia (BPH) and associated lower urinary tract symptoms (LUTS) are common conditions. Little is known about their etiologies except that studies have suggested a substantial heritable component. Our objective is to provide a comprehensive, genome-wide evaluation of inherited risks and possible mechanisms of etiology in BPH. We performed a three-stage, genome-wide association study (GWAS) of men from three independent populations, the REduction by DUtasteride of prostate Cancer Events (REDUCE) trial, the CLUE II cohort, and a Finnish hospital-based population. DNA samples were genotyped using the Illumina HumanOmniExpress BeadChip in REDUCE and CLUE II, and using the Sequenom iPLEX system for the confirmation stage in the Finnish population. A logistic regression model was used to evaluate the association between each SNP and BPH/LUTS. Fourteen SNPs reached P BPH pathogenesis and progression. Rs17144046 located near GATA3 was significantly associated with BPH/LUTS in three independent populations, but did not reach a stringent GWAS significance level. Genetic variants of GATA3 may play a role in the inherited susceptibility and etiology of BPH/LUTS. Further research in this area is needed. © 2017 Wiley Periodicals, Inc.

  4. Plasmacytoid Urothelial Carcinoma of the Urinary Bladder Metastatic to the Duodenum: A Case Report—Diagnostic Relevance of GATA3 Immunohistochemistry

    Directory of Open Access Journals (Sweden)

    Hermann Brustmann

    2017-01-01

    Full Text Available Plasmacytoid urothelial carcinoma (PUC of the urinary bladder is a rare and aggressive subtype of urothelial carcinoma. Its deceptive morphology is characterized by a discohesive growth of cells with plasmacytoid morphology. Since this tumor might be confused with plasmacytoma, lymphoma, or carcinoma variants, appropriate diagnosis in small biopsy samples could be challenging. This study reports the case of a 53-year-old man who presented with frequent nocturnal urgency, without hematuria. A transurethral bladder and a prostate resection specimen displayed infiltration of neoplastic cells in a spray-like discohesive pattern with occasional formation of small irregular nests and cord-like arrangements. The basic morphology of the tumor cells was plasmacytoid, with eccentric nuclei and eosinophilic cytoplasm. Tumor cells grew through the lamina muscularis mucosae, with splintering of the bladder wall musculature and infiltration of prostatic tissue. They displayed strong and diffuse nuclear reactivity for p53 and GATA3. Eight months after surgery, the patient experienced upper abdominal discomfort. A duodenal biopsy showed infiltration of plasmacytoid atypical cells strongly immunoreactive for GATA3, consistent with the previously diagnosed PUC. The patient died eleven months after the primary diagnosis of his PUC of tumor cachexia losing about 50% of his original body weight, furthermore, with ascites and intraperitoneal tumor spread.

  5. Drosophila C-terminal binding protein, dCtBP is required for sensory organ prepattern and sharpens proneural transcriptional activity of the GATA factor Pnr.

    Science.gov (United States)

    Biryukova, Inna; Heitzler, Pascal

    2008-11-01

    The peripheral nervous system is required for animals to detect and to relay environmental stimuli to central nervous system for the information processing. In Drosophila, the precise spatial and temporal expression of two proneural genes achaete (ac) and scute (sc), is necessary for development of the sensory organs. Here we present an evidence that the transcription co-repressor, dCtBP acts as a negative regulator of sensory organ prepattern. Loss of dCtBP function mutant exhibits ectopic sensory organs, while overexpression of dCtBP results in a dramatic loss of sensory organs. These phenotypes are correlated with mis-emerging of sensory organ precursors and perturbated expression of proneural transcription activator Ac. Mammalian CtBP-1 was identified via interaction with the consensus motif PXDLSX(K/R) of adenovirus E1A oncoprotein. We demonstrated that dCtBP binds directly to PLDLS motif of Drosophila Friend of GATA-1 protein, U-shaped and sharpens the adult sensory organ development. Moreover, we found that dCtBP mediates multivalent interaction with the GATA transcriptional activator Pannier and acts as a direct co-repressor of the Pannier-mediated activation of proneural genes. We demonstrated that Pannier genetically interacts with dCtBP-interacting protein HDAC1, suggesting that the dCtBP-dependent regulation of Pannier activity could utilize a repressive mechanism involving alteration of local chromatine structure.

  6. Senp1 drives hypoxia-induced polycythemia via GATA1 and Bcl-xL in subjects with Monge’s disease

    Science.gov (United States)

    Azad, Priti; Zhao, Huiwen W.; Ronen, Roy; Zhou, Dan; Poulsen, Orit; Hsiao, Yu Hsin; Bafna, Vineet

    2016-01-01

    In this study, because excessive polycythemia is a predominant trait in some high-altitude dwellers (chronic mountain sickness [CMS] or Monge’s disease) but not others living at the same altitude in the Andes, we took advantage of this human experiment of nature and used a combination of induced pluripotent stem cell technology, genomics, and molecular biology in this unique population to understand the molecular basis for hypoxia-induced excessive polycythemia. As compared with sea-level controls and non-CMS subjects who responded to hypoxia by increasing their RBCs modestly or not at all, respectively, CMS cells increased theirs remarkably (up to 60-fold). Although there was a switch from fetal to adult HgbA0 in all populations and a concomitant shift in oxygen binding, we found that CMS cells matured faster and had a higher efficiency and proliferative potential than non-CMS cells. We also established that SENP1 plays a critical role in the differential erythropoietic response of CMS and non-CMS subjects: we can convert the CMS phenotype into that of non-CMS and vice versa by altering SENP1 levels. We also demonstrated that GATA1 is an essential downstream target of SENP1 and that the differential expression and response of GATA1 and Bcl-xL are a key mechanism underlying CMS pathology. PMID:27821551

  7. Single-cell-derived mesenchymal stem cells overexpressing Csx/Nkx2.5 and GATA4 undergo the stochastic cardiomyogenic fate and behave like transient amplifying cells

    International Nuclear Information System (INIS)

    Yamada, Yoji; Sakurada, Kazuhiro; Takeda, Yukiji; Gojo, Satoshi; Umezawa, Akihiro

    2007-01-01

    Bone marrow-derived stromal cells can give rise to cardiomyocytes as well as adipocytes, osteocytes, and chondrocytes in vitro. The existence of mesenchymal stem cells has been proposed, but it remains unclear if a single-cell-derived stem cell stochastically commits toward a cardiac lineage. By single-cell marking, we performed a follow-up study of individual cells during the differentiation of 9-15c mesenchymal stromal cells derived from bone marrow cells. Three types of cells, i.e., cardiac myoblasts, cardiac progenitors and multipotent stem cells were differentiated from a single cell, implying that cardiomyocytes are generated stochastically from a single-cell-derived stem cell. We also demonstrated that overexpression of Csx/Nkx2.5 and GATA4, precardiac mesodermal transcription factors, enhanced cardiomyogenic differentiation of 9-15c cells, and the frequency of cardiomyogenic differentiation was increased by co-culturing with fetal cardiomyocytes. Single-cell-derived mesenchymal stem cells overexpressing Csx/Nkx2.5 and GATA4 behaved like cardiac transient amplifying cells, and still retained their plasticity in vivo

  8. Fiscal 2000 achievement report on the venture business assisting type regional consortium - Minor business creation base type. Development of 1-chip multifunctional motion sensor and its application to intelligent module; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo seika hokokusho. 1 chip gata takino undo sensor no kaihatsu to intelligent module eno tekiyo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The aim is to embody an intelligent micromodule for sensing bodily motions. For this purpose, technologies were established for high accuracy/high aspect ratio etching of crystals and for detecting angular velocity and acceleration, and a 1-chip multifunctional motion sensor was developed. The results of the efforts are briefly described below. A 1-chip multifunctional motion sensor (device size: 16 times 6 times 0.3mm) was developed, capable of simultaneously detecting uniaxial acceleration and uniaxial angular velocity, and an operating circuit was established for the detection. Using the 1-chip multifunctional motion sensor, a wrist watch type intelligent module was developed, capable of discriminating between various patterns of human behavior (walking, jogging, desk work, etc.). An intelligent module and the host computer were connected by wire or radio enabling the real-time observation of a patient's kinetic behavior, and this helped develop an application program allowing the quantification of the rate of recovery of patients undergoing rehabilitation. Using an intelligent module, an application program was developed enabling a laryngeal patient to establish communication by a physical action in case of emergency. (NEDO)

  9. Energy saving type area hot water supply system using heat of hot waste water from the sludge center as hot source for hot water; New energy rokko airando CITY. Surajjisenta karano onhaisuinetsu wo kyuyuyo netsugen ni riyosuru sho energy gata chiiki onsui kyokyu system

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-01-01

    Heat source of area hot water supply system in Rokko island City (man-made island) is heat of combustion at the sludge center (sludge incineration plant) in this island. Dehydrated sludge cakes (230ton/day) brought from seven sewage disposal plants in Kobe City is combusted (850degC) in the fluid bed hearth. Combustion gas washed in the scrubber, hot waste water after the washing give heat into heat transfer water in the first heat exchanger. Temperature being 64degC in summer and about 50degC in winter, this heat transfer water is sent into the second heat exchanger at every condominium building throughout the pipe line system circulating in the area. At each home, gas heater and hot water supply devices fitted, additional combustion is not necessary in summer but is used according to demand in other seasons. This hot water supply service has been carried out since 1988 and at present has been used by 3600 homes. Amount of supplying hot water being about 3000cu.m/day, saving is calculated roughly as 60% of gas for hot water supply. Fee for this system is 1500/yen/month uniformly for each home. 14 figs.

  10. In Vivo Interplay between p27Kip1, GATA3, ATOH1, and POU4F3 Converts Non-sensory Cells to Hair Cells in Adult Mice

    Directory of Open Access Journals (Sweden)

    Bradley J. Walters

    2017-04-01

    Full Text Available Summary: Hearing loss is widespread and persistent because mature mammalian auditory hair cells (HCs are nonregenerative. In mice, the ability to regenerate HCs from surrounding supporting cells (SCs declines abruptly after postnatal maturation. We find that combining p27Kip1 deletion with ectopic ATOH1 expression surmounts this age-related decline, leading to conversion of SCs to HCs in mature mouse cochleae and after noise damage. p27Kip1 deletion, independent of canonical effects on Rb-family proteins, upregulated GATA3, a co-factor for ATOH1 that is lost from SCs with age. Co-activation of GATA3 or POU4F3 and ATOH1 promoted conversion of SCs to HCs in adult mice. Activation of POU4F3 alone also converted mature SCs to HCs in vivo. These data illuminate a genetic pathway that initiates auditory HC regeneration and suggest p27Kip1, GATA3, and POU4F3 as additional therapeutic targets for ATOH1-mediated HC regeneration. : Auditory hair cells are nonregenerative, resulting in persistent hearing loss upon damage. Walters et al. find that manipulating two genes, p27Kip1 and Atoh1, induces the conversion of nonsensory cells to hair cells in adult mice. This effect is mediated by GATA3 and POU4F3, where POU4F3 alone was found to convert nonsensory cells. Keywords: regeneration, aging, differentiation, proliferation, development, cancer, sensory, cochlea, hearing

  11. Smad4 mediated BMP2 signal is essential for the regulation of GATA4 and Nkx2.5 by affecting the histone H3 acetylation in H9c2 cells

    International Nuclear Information System (INIS)

    Si, Lina; Shi, Jin; Gao, Wenqun; Zheng, Min; Liu, Lingjuan; Zhu, Jing; Tian, Jie

    2014-01-01

    Highlights: • BMP2 can upregulated cardiac related gene GATA4, Nkx2.5, MEF2c and Tbx5. • Inhibition of Smad4 decreased BMP2-induced hyperacetylation of histone H3. • Inhibition of Smad4 diminished BMP2-induced overexpression of GATA4 and Nkx2.5. • Inhibition of Smad4 decreased hyperacetylated H3 in the promoter of GATA4 and Nkx2.5. • Smad4 is essential for BMP2 induced hyperacetylated histone H3. - Abstract: BMP2 signaling pathway plays critical roles during heart development, Smad4 encodes the only common Smad protein in mammals, which is a pivotal nuclear mediator. Our previous studies showed that BMP2 enhanced the expression of cardiac transcription factors in part by increasing histone H3 acetylation. In the present study, we tested the hypothesis that Smad4 mediated BMP2 signaling pathway is essential for the expression of cardiac core transcription factors by affecting the histone H3 acetylation. We successfully constructed a lentivirus-mediated short hairpin RNA interference vector targeting Smad4 (Lv-Smad4) in rat H9c2 embryonic cardiac myocytes (H9c2 cells) and demonstrated that it suppressed the expression of the Smad4 gene. Cultured H9c2 cells were transfected with recombinant adenoviruses expressing human BMP2 (AdBMP2) with or without Lv-Smad4. Quantitative real-time RT-PCR analysis showed that knocking down of Smad4 substantially inhibited both AdBMP2-induced and basal expression levels of cardiac transcription factors GATA4 and Nkx2.5, but not MEF2c and Tbx5. Similarly, chromatin immunoprecipitation (ChIP) analysis showed that knocking down of Smad4 inhibited both AdBMP2-induced and basal histone H3 acetylation levels in the promoter regions of GATA4 and Nkx2.5, but not of Tbx5 and MEF2c. In addition, Lv-Smad4 selectively suppressed AdBMP2-induced expression of HAT p300, but not of HAT GCN5 in H9c2 cells. The data indicated that inhibition of Smad4 diminished both AdBMP2 induced and basal histone acetylation levels in the promoter regions of

  12. Smad4 mediated BMP2 signal is essential for the regulation of GATA4 and Nkx2.5 by affecting the histone H3 acetylation in H9c2 cells

    Energy Technology Data Exchange (ETDEWEB)

    Si, Lina; Shi, Jin; Gao, Wenqun [Heart Centre, Children’s Hospital of Chongqing Medical University, 136 Zhongshan 2nd Road, Yu Zhong District, Chongqing 400014 (China); Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, 136 Zhongshan 2nd Road, Yu Zhong District, Chongqing 400014 (China); Zheng, Min [Heart Centre, Children’s Hospital of Chongqing Medical University, 136 Zhongshan 2nd Road, Yu Zhong District, Chongqing 400014 (China); Liu, Lingjuan; Zhu, Jing [Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, 136 Zhongshan 2nd Road, Yu Zhong District, Chongqing 400014 (China); Tian, Jie, E-mail: jietian@cqmu.edu.cn [Heart Centre, Children’s Hospital of Chongqing Medical University, 136 Zhongshan 2nd Road, Yu Zhong District, Chongqing 400014 (China)

    2014-07-18

    Highlights: • BMP2 can upregulated cardiac related gene GATA4, Nkx2.5, MEF2c and Tbx5. • Inhibition of Smad4 decreased BMP2-induced hyperacetylation of histone H3. • Inhibition of Smad4 diminished BMP2-induced overexpression of GATA4 and Nkx2.5. • Inhibition of Smad4 decreased hyperacetylated H3 in the promoter of GATA4 and Nkx2.5. • Smad4 is essential for BMP2 induced hyperacetylated histone H3. - Abstract: BMP2 signaling pathway plays critical roles during heart development, Smad4 encodes the only common Smad protein in mammals, which is a pivotal nuclear mediator. Our previous studies showed that BMP2 enhanced the expression of cardiac transcription factors in part by increasing histone H3 acetylation. In the present study, we tested the hypothesis that Smad4 mediated BMP2 signaling pathway is essential for the expression of cardiac core transcription factors by affecting the histone H3 acetylation. We successfully constructed a lentivirus-mediated short hairpin RNA interference vector targeting Smad4 (Lv-Smad4) in rat H9c2 embryonic cardiac myocytes (H9c2 cells) and demonstrated that it suppressed the expression of the Smad4 gene. Cultured H9c2 cells were transfected with recombinant adenoviruses expressing human BMP2 (AdBMP2) with or without Lv-Smad4. Quantitative real-time RT-PCR analysis showed that knocking down of Smad4 substantially inhibited both AdBMP2-induced and basal expression levels of cardiac transcription factors GATA4 and Nkx2.5, but not MEF2c and Tbx5. Similarly, chromatin immunoprecipitation (ChIP) analysis showed that knocking down of Smad4 inhibited both AdBMP2-induced and basal histone H3 acetylation levels in the promoter regions of GATA4 and Nkx2.5, but not of Tbx5 and MEF2c. In addition, Lv-Smad4 selectively suppressed AdBMP2-induced expression of HAT p300, but not of HAT GCN5 in H9c2 cells. The data indicated that inhibition of Smad4 diminished both AdBMP2 induced and basal histone acetylation levels in the promoter regions of

  13. GATA4 Variants in Individuals With a 46,XY Disorder of Sex Development (DSD May or May Not Be Associated With Cardiac Defects Depending on Second Hits in Other DSD Genes

    Directory of Open Access Journals (Sweden)

    Idoia Martinez de LaPiscina

    2018-04-01

    Full Text Available Disorders of sex development (DSD consist of a wide range of conditions involving numerous genes. Nevertheless, about half of 46,XY individuals remain genetically unsolved. GATA4 gene variants, mainly related to congenital heart defects (CHD, have also been recently associated with 46,XY DSD. In this study, we characterized three individuals presenting with 46,XY DSD with or without CHD and GATA4 variants in order to understand the phenotypical variability. We studied one patient presenting CHD and 46,XY gonadal dysgenesis, and two patients with a history of genetically unsolved 46,XY DSD, also known as male primary hypogonadism. Mutation analysis was carried out by candidate gene approach or targeted gene panel sequencing. Functional activity of GATA4 variants was tested in vitro on the CYP17 promoter involved in sex development using JEG3 cells. We found two novel and one previously described GATA4 variants located in the N-terminal zinc finger domain of the protein. Cys238Arg variant lost transcriptional activity on the CYP17 promoter reporter, while Trp228Cys and Pro226Leu behaved similar to wild type. These results were in line with bioinformatics simulation studies. Additional DSD variations, in the LRP4 and LHCGR genes, respectively, were identified in the two 46,XY individuals without CHD. Overall, our study shows that human GATA4 mutations identified in patients with 46,XY DSD may or may not be associated with CHD. Possible explanations for phenotypical variability may comprise incomplete penetrance, variable sensitivity of partner genes, and oligogenic mechanisms.

  14. Method of determination of the leaf area of the potato cultivar Ágata from linear dimensionsMétodo de determinação da área foliar da cultivar de batata Ágata a partir de dimensões lineares

    Directory of Open Access Journals (Sweden)

    Sidnei Osmar Jadoski

    2012-12-01

    Full Text Available The cultivar Ágata is currently the most widely cultivated in areas of potato production in Brazil, however, are necessaries basic information about its characteristics, especially on the development of the canopy. The work was developed in experimental area of the Unit of Research in Potato Culture and Microclimate for Agriculture, in the department of Agronomy of the UniversidadeEstadual do Centro Oeste (Unicentro, in Guarapuava (PR. The aim was to evaluate the efficiency of the adjustment of different mathematic models to determine the leaf area of potato plants of the cultivar Ágata. The evaluations were performed considering the dimensions of a 100 seeds of several sizes which were randomly collected in the phase of maximum vegetative development of plants. The data obtained were submitted to analysis of regression, being tested six different mathematic models: linear, quadratic, cubic functions and the exponential models from growth, Gauss and Lorentz, using the length and the height of leaves as independent variable to the adjustment in function of the real leaf area measured in laboratory. The variable length and the product of length x width of the leaf were those who presented the best results of adjustments to the models tested. With more emphasis to the length, for which all the models testes present statistical significance. It was concluded that the leaf length is the most appropriated parameter to be used in evaluation of the leaf area of potato plants cultivar Ágata, being the exponential function of growth and Lorentz the less recommended since its lowest adjustment coefficient. A cultivar Ágata é atualmente a mais cultivada nas lavouras de produção de batata no Brasil, contudo, ainda são necessárias informações básicas sobre suas características, especialmente sobre o desenvolvimento do dossel vegetativo.O trabalho foi desenvolvido em área experimental da Unidade de Pesquisa em Bataticultura e Microclima para a

  15. Xenotransplante ovariano de gatas domésticas em camundongas C57BL/6 SCID e sua resposta á gonadotrofina coriõnica equina

    OpenAIRE

    Santos, Fernanda Araujo dos

    2015-01-01

    Xenotransplante ovariano é uma técnica reprodutiva auxiliar que permite a conservação do germoplasma de espécies de alto valor zootécnico ou em perigo de extinção. O uso de gonadotrofinas exógenas auxilia no desenvolvimento desses tecidos xenotransplantados e na obtenção de folículos viáveis para produção in vitro de embriões (PIVE), entretanto esse uso não foi relatado em xenotransplante de ovários de gatas com fêmeas C57BL/6 SCID como receptora. Dessa forma, o objetivo desse trabalho ...

  16. INDUÇÃO DE OVULAÇÃO COM SWAB VAGINAL EM GATAS DOMÉSTICAS E SEUS EFEITOS SOBRE A MORFOLOGIA UTERINA

    OpenAIRE

    Silva, Sandra Cristina Becker; Silva, Maíra Corona da; Silva, Fabiana Lessa; Snoeck, Paola Pereira das Neves

    2017-01-01

    Resumo A ovulação em gatas é induzida por um reflexo neuroendócrino atribuído à estimulação mecânica dos receptores sensoriais durante o coito. Esta estimulação pode ser simulada com auxílio do swab vaginal, desencadeando a pseudogestação. Objetivou-se verificar a eficiência da indução de ovulação com swab, a fim de estabelecer um tratamento contraceptivo natural para felinos domésticos, bem como os efeitos sobre o útero do uso repetido dessa técnica. Na primeira fase do trabalho, foram avali...

  17. Stable T-bet+GATA-3+ Th1/Th2 Hybrid Cells Arise In Vivo, Can Develop Directly from Naive Precursors, and Limit Immunopathologic Inflammation

    Science.gov (United States)

    Peine, Michael; Fröhlich, Anja; Hegazy, Ahmed N.; Kühl, Anja A.; Grevelding, Christoph G.; Höfer, Thomas; Hartmann, Susanne; Löhning, Max

    2013-01-01

    Differentiated T helper (Th) cell lineages are thought to emerge from alternative cell fate decisions. However, recent studies indicated that differentiated Th cells can adopt mixed phenotypes during secondary immunological challenges. Here we show that natural primary immune responses against parasites generate bifunctional Th1 and Th2 hybrid cells that co-express the lineage-specifying transcription factors T-bet and GATA-3 and co-produce Th1 and Th2 cytokines. The integration of Th1-promoting interferon (IFN)-γ and interleukin (IL)-12 signals together with Th2-favoring IL-4 signals commits naive Th cells directly and homogeneously to the hybrid Th1/2 phenotype. Specifically, IFN-γ signals are essential for T-bet+GATA-3+ cells to develop in vitro and in vivo by breaking the dominance of IL-4 over IL-12 signals. The hybrid Th1/2 phenotype is stably maintained in memory cells in vivo for months. It resists reprogramming into classic Th1 or Th2 cells by Th1- or Th2-promoting stimuli, which rather induce quantitative modulations of the combined Th1 and Th2 programs without abolishing either. The hybrid phenotype is associated with intermediate manifestations of both Th1 and Th2 cell properties. Consistently, hybrid Th1/2 cells support inflammatory type-1 and type-2 immune responses but cause less immunopathology than Th1 and Th2 cells, respectively. Thus, we propose the self-limitation of effector T cells based on the stable cell-intrinsic balance of two opposing differentiation programs as a novel concept of how the immune system can prevent excessive inflammation. PMID:23976880

  18. C. elegans GATA factors EGL-18 and ELT-6 function downstream of Wnt signaling to maintain the progenitor fate during larval asymmetric divisions of the seam cells.

    Science.gov (United States)

    Gorrepati, Lakshmi; Thompson, Kenneth W; Eisenmann, David M

    2013-05-01

    The C. elegans seam cells are lateral epithelial cells arrayed in a single line from anterior to posterior that divide in an asymmetric, stem cell-like manner during larval development. These asymmetric divisions are regulated by Wnt signaling; in most divisions, the posterior daughter in which the Wnt pathway is activated maintains the progenitor seam fate, while the anterior daughter in which the Wnt pathway is not activated adopts a differentiated hypodermal fate. Using mRNA tagging and microarray analysis, we identified the functionally redundant GATA factor genes egl-18 and elt-6 as Wnt pathway targets in the larval seam cells. EGL-18 and ELT-6 have previously been shown to be required for initial seam cell specification in the embryo. We show that in larval seam cell asymmetric divisions, EGL-18 is expressed strongly in the posterior seam-fated daughter. egl-18 and elt-6 are necessary for larval seam cell specification, and for hypodermal to seam cell fate transformations induced by ectopic Wnt pathway overactivation. The TCF homolog POP-1 binds a site in the egl-18 promoter in vitro, and this site is necessary for robust seam cell expression in vivo. Finally, larval overexpression of EGL-18 is sufficient to drive expression of a seam marker in other hypodermal cells in wild-type animals, and in anterior hypodermal-fated daughters in a Wnt pathway-sensitized background. These data suggest that two GATA factors that are required for seam cell specification in the embryo independently of Wnt signaling are reused downstream of Wnt signaling to maintain the progenitor fate during stem cell-like divisions in larval development.

  19. Stable T-bet(+GATA-3(+ Th1/Th2 hybrid cells arise in vivo, can develop directly from naive precursors, and limit immunopathologic inflammation.

    Directory of Open Access Journals (Sweden)

    Michael Peine

    Full Text Available Differentiated T helper (Th cell lineages are thought to emerge from alternative cell fate decisions. However, recent studies indicated that differentiated Th cells can adopt mixed phenotypes during secondary immunological challenges. Here we show that natural primary immune responses against parasites generate bifunctional Th1 and Th2 hybrid cells that co-express the lineage-specifying transcription factors T-bet and GATA-3 and co-produce Th1 and Th2 cytokines. The integration of Th1-promoting interferon (IFN-γ and interleukin (IL-12 signals together with Th2-favoring IL-4 signals commits naive Th cells directly and homogeneously to the hybrid Th1/2 phenotype. Specifically, IFN-γ signals are essential for T-bet(+GATA-3(+ cells to develop in vitro and in vivo by breaking the dominance of IL-4 over IL-12 signals. The hybrid Th1/2 phenotype is stably maintained in memory cells in vivo for months. It resists reprogramming into classic Th1 or Th2 cells by Th1- or Th2-promoting stimuli, which rather induce quantitative modulations of the combined Th1 and Th2 programs without abolishing either. The hybrid phenotype is associated with intermediate manifestations of both Th1 and Th2 cell properties. Consistently, hybrid Th1/2 cells support inflammatory type-1 and type-2 immune responses but cause less immunopathology than Th1 and Th2 cells, respectively. Thus, we propose the self-limitation of effector T cells based on the stable cell-intrinsic balance of two opposing differentiation programs as a novel concept of how the immune system can prevent excessive inflammation.

  20. Caracterização química e física de batatas ágata e monalisa minimamente processadas Chemical and physical characterization of fresh-cut potatoes

    Directory of Open Access Journals (Sweden)

    Lívia L. O. Pineli

    2006-03-01

    Full Text Available O objetivo deste trabalho foi caracterizar química e fisicamente batatas minimamente processadas durante o armazenamento refrigerado. Batatas (Solanum tuberosum, L. ágata e monalisa foram minimamente processadas como minibatatas. Após o processamento, as batatas foram acondicionadas sob vácuo parcial e, posteriormente, armazenadas em câmaras frias a 5 e 15ºC, por nove dias. A cada três dias, foram avaliadas as seguintes variáveis: firmeza, atividade da polifenoloxidase e peroxidase, açúcares solúveis totais, amido e vitamina C total. Nas batatas armazenadas a 15ºC, constatou-se que, após nove dias de armazenamento, sua firmeza era 3,3 vezes menor em batatas monalisa e 4,3 vezes menor para a cultivar ágata, quando comparadas com o produto recém-processado. A atividade da polifenoloxidase mostrou-se praticamente estável em batatas monalisa armazenadas a 5ºC. Batatas monalisa minimamente processadas apresentaram maior atividade da peroxidase a 5ºC, sendo 86% maior do que a atividade desta enzima em batatas ágata ao final do período experimental. O teor inicial de açúcares solúveis totais nas batatas minimamente processadas era 28% maior na cultivar monalisa, quando comparada com tubérculos de ágata. As duas cultivares apresentaram tendência de elevação do teor de amido nos primeiros três dias, para as duas temperaturas estudadas. Em ambas as cultivares, armazenamento a 5ºC possibilitou maior manutenção dos teores de vitamina C.The present work was carried out aiming to evaluate chemical and physical characteristics of fresh-cut potatoes stored under 5 and 15°C. Potatoes, (Solanum tuberosum L. ágata and Monalisa were minimally processed as baby potatoes. After processing, the material was packed under partial vacuum and stored at 5 and 15°C for nine days. Every three days tubers were evaluated for firmness, enzymatic activity (polyphenoloxidase and peroxidase, total soluble sugars, starch, and total vitamin C

  1. Vasohibin 2 promotes epithelial-mesenchymal transition in human breast cancer via activation of transforming growth factor β 1 and hypoxia dependent repression of GATA-binding factor 3.

    Science.gov (United States)

    Tu, Min; Li, Zhanjun; Liu, Xian; Lv, Nan; Xi, Chunhua; Lu, Zipeng; Wei, Jishu; Song, Guoxin; Chen, Jianmin; Guo, Feng; Jiang, Kuirong; Wang, Shui; Gao, Wentao; Miao, Yi

    2017-03-01

    Vasohibin 2 (VASH2) is identified as an angiogenic factor, and has been implicated in tumor angiogenesis, proliferation and epithelial-mesenchymal transition (EMT). To investigate the EMT role of VASH2 in breast cancer, we overexpressed or knocked down expression of VASH2 in human breast cancer cell lines. We observed that VASH2 induced EMT in vitro and in vivo. The transforming growth factor β1 (TGFβ1) pathway was activated by VASH2, and expression of a dominant negative TGFβ type II receptor could block VASH2-mediated EMT. In clinical breast cancer tissues VASH2 positively correlated with TGFβ1 expression, but negatively correlated with E-cadherin (a marker of EMT) expression. Under hypoxic conditions in vitro or in vivo, we found that down-regulation of estrogen receptor 1 (ESR1) in VASH2 overexpressing ESR1 positive cells suppressed E-cadherin. Correlation coefficient analysis indicated that VASH2 and ESR1 expression were negatively correlated in clinical human breast cancer tissues. Further study revealed that a transcription factor of ESR1, GATA-binding factor 3 (GATA3), was down-regulated by VASH2 under hypoxia or in vivo. These findings suggest that VASH2 drives breast cancer cells to undergo EMT by activation of the TGFβ1 pathway and hypoxia dependent repression GATA3-ESR1 pathway, leading to cancer metastasis. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  2. The +37 kb Cebpa Enhancer Is Critical for Cebpa Myeloid Gene Expression and Contains Functional Sites that Bind SCL, GATA2, C/EBPα, PU.1, and Additional Ets Factors

    Science.gov (United States)

    Cooper, Stacy; Guo, Hong; Friedman, Alan D.

    2015-01-01

    The murine Cebpa gene contains an evolutionarily conserved 453 bp enhancer located at +37 kb that, together with its promoter, directs expression to myeloid progenitors and to long-term hematopoietic stem cells in transgenic mice. In human acute myeloid leukemia cases, the enhancer lacks point mutations but binds the RUNX1-ETO oncoprotein. The enhancer contains the H3K4me1 and H3K27Ac histone modifications, denoting an active enhancer, at progressively increasing levels as long-term hematopoietic stem cells transition to granulocyte-monocyte progenitors. We previously identified four enhancer sites that bind RUNX1 and demonstrated that their integrity is required for maximal enhancer activity in 32Dcl3 myeloid cells. The +37 kb Cebpa enhancer also contains C/EBP, Ets factor, Myb, GATA, and E-box consensus sites conserved in the human +42 kb CEBPA enhancer. Mutation of the two C/EBP, seven Ets, one Myb, two GATA, or two E-box sites reduces activity of an enhancer-promoter reporter in 32Dcl3 cells. In 293T gel shift assays, exogenous C/EBPα binds both C/EBP sites, c-Myb binds the Myb site, PU.1 binds the second Ets site, PU.1, Fli-1, ERG, and Ets1 bind the sixth Ets site, GATA2 binds both GATA sites, and SCL binds the second E-box. Endogenous hematopoietic RUNX1, PU.1, Fli-1, ERG, C/EBPα, GATA2, and SCL were previously shown to bind the enhancer, and we find that endogenous PU.1 binds the second Ets site in 32Dcl3 cells. Using CRISPR/Cas9, we developed 32Dcl3 lines in which the wild-type enhancer alleles are replaced with a variant mutant in the seven Ets sites. These lines have 20-fold reduced Cebpa mRNA when cultured in IL-3 or G-CSF, demonstrating a critical requirement for enhancer integrity for optimal Cebpa expression. In addition, these results indicate that the +37 kb Cebpa enhancer is the focus of multiple regulatory transcriptional pathways that impact its expression during normal hematopoiesis and potentially during myeloid transformation. PMID:25938608

  3. Caracterização química e física de batatas 'Ágata' minimamente processadas, embaladas sob diferentes atmosferas modificadas ativas Chemical and physical characterization of fresh-cut 'Ágata' potatoes packed under different active modified atmospheres

    Directory of Open Access Journals (Sweden)

    Lívia Lacerda Oliveira Pineli

    2005-10-01

    Full Text Available O objetivo deste trabalho foi avaliar as alterações químicas e físicas em batatas 'Ágata' minimamente processadas, embaladas sob diferentes atmosferas, durante o armazenamento refrigerado. Batatas 'Ágata' foram minimamente processadas como minibatatas e embaladas em filmes de náilon multicamadas. Os tratamentos aplicados no momento da embalagem foram o vácuo parcial e as misturas 10%CO2, 2%O2, 88%N2 ou 5%CO2, 5%O2, 90%N2. Em seguida, foram armazenadas a 5ºC. A cada três dias, amostras foram avaliadas quanto ao índice de escurecimento, atividade da polifenoloxidase e peroxidase, firmeza, sólidos solúveis totais e acidez titulável. O armazenamento sob vácuo parcial foi o mais eficaz no controle do escurecimento, da minimização da atividade da polifenoloxidase e da peroxidase, prevenindo alterações nos teores de sólidos solúveis totais, redução da firmeza, encharcamento da embalagem e maior acidez titulável. Os demais tratamentos apresentaram rápido desenvolvimento do escurecimento, aumento de firmeza em decorrência de ressecamento dos tubérculos, elevação nos sólidos solúveis totais e menor desenvolvimento da acidez em comparação ao tratamento sob vácuo parcial. A utilização de vácuo parcial foi a mais recomendada para a manutenção da qualidade das minibatatas. Todavia, outros tratamentos que busquem a manutenção da firmeza e frescor, sem o desenvolvimento de off-flavors e sem escurecimento, devem ser avaliados.The objective of this work was to evaluate chemical and physical characteristics of fresh-cut 'Ágata' potatoes packed under different active modified atmospheres, during refrigerated storage. 'Ágata' potatoes were minimally processed as baby potatoes and packed in nylon multilayer films. The treatments applied were partial vacuum, and atmospheres with 10%CO2, 2%O2, 88%N2 and 5%CO2, 5%O2, 90%N2. After that, they were stored at 5ºC. Every three days, tubers were evaluated for browning index

  4. Cooperative action of multiple cis-acting elements is required for N-myc expression in branchial arches: specific contribution of GATA3.

    Science.gov (United States)

    Potvin, Eric; Beuret, Laurent; Cadrin-Girard, Jean-François; Carter, Marcelle; Roy, Sophie; Tremblay, Michel; Charron, Jean

    2010-11-01

    The precise expression of the N-myc proto-oncogene is essential for normal mammalian development, whereas altered N-myc gene regulation is known to be a determinant factor in tumor formation. Using transgenic mouse embryos, we show that N-myc sequences from kb -8.7 to kb +7.2 are sufficient to reproduce the N-myc embryonic expression profile in developing branchial arches and limb buds. These sequences encompass several regulatory elements dispersed throughout the N-myc locus, including an upstream limb bud enhancer, a downstream somite enhancer, a branchial arch enhancer in the second intron, and a negative regulatory element in the first intron. N-myc expression in the limb buds is under the dominant control of the limb bud enhancer. The expression in the branchial arches necessitates the interplay of three regulatory domains. The branchial arch enhancer cooperates with the somite enhancer region to prevent an inhibitory activity contained in the first intron. The characterization of the branchial arch enhancer has revealed a specific role of the transcription factor GATA3 in the regulation of N-myc expression. Together, these data demonstrate that correct N-myc developmental expression is achieved via cooperation of multiple positive and negative regulatory elements.

  5. The C. elegans embryonic fate specification factor EGL-18 (GATA) is reutilized downstream of Wnt signaling to maintain a population of larval progenitor cells.

    Science.gov (United States)

    Gorrepati, Lakshmi; Eisenmann, David M

    2015-01-01

    In metazoans, stem cells in developing and adult tissues can divide asymmetrically to give rise to a daughter that differentiates and a daughter that retains the progenitor fate. Although the short-lived nematode C. elegans does not possess adult somatic stem cells, the lateral hypodermal seam cells behave in a similar manner: they divide once per larval stage to generate an anterior daughter that adopts a non-dividing differentiated fate and a posterior daughter that retains the seam fate and the ability to divide further. Wnt signaling pathway is known to regulate the asymmetry of these divisions and maintain the progenitor cell fate in one daughter, but how activation of the Wnt pathway accomplished this was unknown. We describe here our recent work that identified the GATA transcription factor EGL-18 as a downstream target of Wnt signaling necessary for maintenance of a progenitor population of larval seam cells. EGL-18 was previously shown to act in the initial specification of the seam cells in the embryo. Thus the acquisition of a Wnt-responsive cis-regulatory module allows an embryonic fate specification factor to be reutilized later in life downstream of a different regulator (Wnt signaling) to maintain a progenitor cell population. These results support the use of seam cell development in C. elegans as a simple model system for studying stem and progenitor cell biology.

  6. A novel in vivo adjuvant activity of kaempferol: enhanced Tbx-21, GATA-3 expression and peritoneal CD11c+MHCII+ dendritic cell infiltration.

    Science.gov (United States)

    Singh, Divya; Tanwar, Himanshi; Das, Sudeshna; Ganju, Lilly; Singh, Shashi Bala

    2018-02-28

    Kaempferol, a natural flavonol present in various traditional medicinal plants, is known to possess potent anti-inflammatory properties. This study was designed to study the adjuvant effect of kaempferol administration along with ovalbumin antigen (K + O) in balb/c mice. Mice were immunized with kaempferol (100 and 50 mg/kg body weight) without or with ovalbumin (20 µg/mouse). After priming, booster was administered on day 21. Antigen specific IgG titers and its subtypes, on day 28, were estimated by indirect ELISA. Effect of kaempferol administration on CD11c + MHCII + peritoneal dendritic cells was studied by flow cytometry. Expression levels of proteins Tbx21, GATA-3, BLIMP-1, Caspase-1 and Oct-2 were studied by western blotting. LPS activated IL-1β production by peritoneal cells of immunized mice was estimated by sandwich ELISA. Ovalbumin specific IgG, IgG1 and IgG2a antibody titers in sera samples of K + O immunized mice increased significantly (p Kaempferol increased the infiltration of peritoneal CD11c + MHCII + dendritic cells but failed to enhance LPS activated IL-1β by peritoneal macrophages and suppressed caspase-1 protein expression as compared to that in ovalbumin immunized mice. Present study strongly demonstrates the novel adjuvant activity of kaempferol in vivo and its potential as an immunostimulatory agent.

  7. Needs/seeds research committee of welfare apparatus; Fukushi kiki needs seeds tekigo chosa kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    As to the development of welfare apparatus, a study was made on the present status of the periphery environment, problems, measures and policies. As subjects in production, the following were pointed out: small scale of the market, lots of brands/small quantity of production, various corporate sizes in the industry, no competition among countries, modular structuring, etc. As to the standardization, the necessity was asserted for the following: rationalization of production/consumption, security of product quality, provision of standards for accurate judgement of the purchase, simplification of inspection under the Pharmaceutical Law, benefit systems. In relation to evaluation/technical guidance, details of the evaluation, persons involved in the evaluation, and the evaluation form were arranged including not only the evaluation of individual tools, but grasp of the needs, selection of themes, and technical guidance under the development. The fundamental development of the welfare apparatus is being conducted in universities, national research institutes, etc., and necessities were pointed out for a study of trial use of the apparatus as a step next to the prototype making, and a system to support makers for the period from after the development up to the commercialization. 5 refs., 8 figs., 26 tabs.

  8. Surface modification of biocompatible materials. Seitai tekigo zairyo no hyomen kaishitsu

    Energy Technology Data Exchange (ETDEWEB)

    Tateishi, T [Mechanical Engineering Lab., Tsukuba, Ibaraki (Japan)

    1993-07-05

    The necessary conditions for biocompatible materials such as human bone, joints and teeth are mainly classified into biological condition and mechanical conditions. The former condition is consisted of chemical stability without causing poisoning or allergy, compatibility of good biological tissue, no carcinogenesis and not antigenicity, no decomposition and degradation inside human body, not causing adsorbate or precipitate. As for the latter, appropriate static strength, elastic modulus and hardness together with the characteristics like fatigue resistance, wear resistance and lubricating properties are given. As for other conditions for biomaterials, characteristics as a functional material, workability, adhesion and so forth are important. When surface nitrification of sintered Ti, forging Ti, sintered Ti-6Al-4V and forging Ti-6Al-4V is carried out, the solubility is decreased significantly by surface nitrification. The powder generated by the wear of nitriding Ti-6Al-4V with ultra high molecular weight polyethylene (UHMWPE) is less cell poisoning than the powder caused by the wear of the other alumina, stabilized zirconia, hydroxy apatite with UHMWPE. 16 refs., 2 figs., 1 tab.

  9. Investigational research on welfare equipment needs/seeds adaptation; Fukushi kiki needs seeds tekigo chosa kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-03-01

    At the advent of the aging society, investigated are images of welfare equipment to be developed. In the range where the use of welfare equipment is to be studied, the following are included: self care, excretion, transfer, and support of work other than care, as assistance to daily life; exercise function, communication function, and environment arrangement, as assistance to social participation. Of the images of the welfare equipment classified by the overall evaluation, the following are cited as what supports daily life: a lift usable even in the existing houses, a bump remover by which bumps in the house are removed without making large repairs, a urine collector for females, a training device to prevent urinary incontinence, reduction in the bed-fast elderly, a device to prevent bedsore, a system for safely watching demented old people, a system in which clothes are automatically washed, dried, and hanged on a hanger, etc. What supports social participation are a system for sports experience by virtual reality, sound user interface, etc. For the aged to be healthy physically and mentally, it is essential to be taken good care of and create things to live for by participating the society. For this, it is necessary that the government establish the public policies and that a people be conscious of welfare. 9 figs., 33 tabs.

  10. Eletroacupuntura e morfina sobre parâmetros cardiorrespiratórios em gatas submetidas à ovariosalpingohisterectomia eletiva Electroacupuncture and morphine on cardiorespiratory parameters on cat elective ovariohysterectomy

    Directory of Open Access Journals (Sweden)

    Marilda Onghero Taffarel

    2011-12-01

    Full Text Available Objetivou-se avaliar os efeitos da morfina e da eletroacupuntura sobre parâmetros cardiorrespiratórios e consumo do anestésico isofluorano em gatas submetidas à ovariosalpingohisterectomia eletiva. Foram utilizadas 18 gatas hígidas, adultas, distribuídas em três grupos: Eletroacupuntura, Morfina e Controle, as quais receberam acepromazina, propofol e isoflurano. Nos animais dos grupos Controle e Morfina foram introduzidas agulhas em acupontos falsos, e nos do grupo Eletroacupuntura nos pontos Zusanli e Yanglingquan. A eletroestimulação no grupo submetido a Eletroacupuntura foi realizada na frequência de 2 e 100Hz. Nos animais do grupo Morfina, administrou-se a dose de 0,3mg/kg por via intramuscular e no grupo Controle não foi realizado tratamento. O registro das variáveis foi obtido anteriormente à administração da acepromazina; 10 minutos após a aplicação desse fármaco; após indução e estabilização anestésica; decorridos 30 minutos do início da eletroestimulação ou aplicação da morfina; e a cada 10 minutos, durante 60 minutos. Foram estudadas temperatura retal, frequência respiratória e cardíaca, saturação parcial de oxiemoglobina, pressão arterial média, volume total de isoflurano consumido e volume inspirado de isoflurano. Os dados foram submetidos a Análise de Perfil. Somente os animais do grupo Eletroacupuntura (GE não apresentaram diferença significativa entre os momentos para a pressão arterial média, e observou-se melhor estabilidade cardíaca nos animais do grupo Eletroacupuntura e Morfina. O volume inspirado de isoflurano foi 58,33% menor no grupo Eletroacupuntura e 22,02% no grupo Morfina quando comparados ao grupo Controle. Pode-se concluir que eletroestimulação dos acupontos Zusanli e Yanglingquan, diminui o volume inspirado de isoflurano e promove estabilidade cardiorrespiratória, sendo superior ao uso da morfina.The aim of this work was to evaluate the morphine and electroacupuncture

  11. Friend of GATA (FOG interacts with the nucleosome remodeling and deacetylase complex (NuRD to support primitive erythropoiesis in Xenopus laevis.

    Directory of Open Access Journals (Sweden)

    Mizuho S Mimoto

    Full Text Available Friend of GATA (FOG plays many diverse roles in adult and embryonic hematopoiesis, however the mechanisms by which it functions and the roles of potential interaction partners are not completely understood. Previous work has shown that overexpression of FOG in Xenopus laevis causes loss of blood suggesting that in contrast to its role in mammals, FOG might normally function to repress erythropoiesis in this species. Using loss-of-function analysis, we demonstrate that FOG is essential to support primitive red blood cell (RBC development in Xenopus. Moreover, we show that it is specifically required to prevent excess apoptosis of circulating primitive RBCs and that in the absence of FOG, the pro-apoptotic gene Bim-1 is strongly upregulated. To identify domains of FOG that are essential for blood development and, conversely, to begin to understand the mechanism by which overexpressed FOG represses primitive erythropoiesis, we asked whether FOG mutants that are unable to interact with known co-factors retain their ability to rescue blood formation in FOG morphants and whether they repress erythropoiesis when overexpressed in wild type embryos. We find that interaction of FOG with the Nucleosome Remodeling and Deacetylase complex (NuRD, but not with C-terminal Binding Protein, is essential for normal primitive RBC development. In contrast, overexpression of all mutant and wild type constructs causes a comparable repression of primitive erythropoiesis. Together, our data suggest that a requirement for FOG and its interaction with NuRD during primitive erythropoiesis are conserved in Xenopus and that loss of blood upon FOG overexpression is due to a dominant-interfering effect.

  12. Vascularização arterial dos cornos uterinos em gatas gestantes sem raça definida (Felis catus Linnaeus, 1758

    Directory of Open Access Journals (Sweden)

    Rosana Marques Silva

    2004-02-01

    Full Text Available Utilizaram-se 24 gatas gestantes, sem raça definida, doadas para o estudo da vascularização arterial dos cornos uterinos. Em 20 animais, com o intervalo gestacional entre 7 e 9 semanas, a aorta abdominal foi injetada com Látex-Neoprene 650 corado, associado ao Sulfato de Bário 1004. Os animais foram então radiografados, fixados em solução aquosa de formol 10% e dissecados para estudo da distribuição dos vasos arteriais destinados às regiões paraplacentárias e de cintas placentárias. Em 4 animais realizou-se o exame por Ecografia Power Doppler, onde se observou que nas regiões das cintas placentárias o índice de resistência dos vasos apresenta-se menor do que o encontrado ao longo da artéria uterina. Em todas as observações, a artéria uterina é o principal vaso a irrigar os cornos uterinos, emitindo de 2 a 17 ramos, com maior freqüência de 5 a 12 ramos, os quais se distribuem nas regiões paraplacentárias e/ou das cintas placentárias, formando arcadas anastomósticas. Não houve diferenças estatísticas significativas do número de ramos para cada região individualmente, porém a análise das radiografias contrastadas demonstrou haver uma concentração maior de contraste nas regiões das cintas placentárias. A artéria ovárica participa da vascularização arterial da extremidade cranial dos cornos uterinos, onde se anastomosa com ramos da artéria uterina.

  13. Friend of GATA (FOG) interacts with the nucleosome remodeling and deacetylase complex (NuRD) to support primitive erythropoiesis in Xenopus laevis.

    Science.gov (United States)

    Mimoto, Mizuho S; Christian, Jan L

    2012-01-01

    Friend of GATA (FOG) plays many diverse roles in adult and embryonic hematopoiesis, however the mechanisms by which it functions and the roles of potential interaction partners are not completely understood. Previous work has shown that overexpression of FOG in Xenopus laevis causes loss of blood suggesting that in contrast to its role in mammals, FOG might normally function to repress erythropoiesis in this species. Using loss-of-function analysis, we demonstrate that FOG is essential to support primitive red blood cell (RBC) development in Xenopus. Moreover, we show that it is specifically required to prevent excess apoptosis of circulating primitive RBCs and that in the absence of FOG, the pro-apoptotic gene Bim-1 is strongly upregulated. To identify domains of FOG that are essential for blood development and, conversely, to begin to understand the mechanism by which overexpressed FOG represses primitive erythropoiesis, we asked whether FOG mutants that are unable to interact with known co-factors retain their ability to rescue blood formation in FOG morphants and whether they repress erythropoiesis when overexpressed in wild type embryos. We find that interaction of FOG with the Nucleosome Remodeling and Deacetylase complex (NuRD), but not with C-terminal Binding Protein, is essential for normal primitive RBC development. In contrast, overexpression of all mutant and wild type constructs causes a comparable repression of primitive erythropoiesis. Together, our data suggest that a requirement for FOG and its interaction with NuRD during primitive erythropoiesis are conserved in Xenopus and that loss of blood upon FOG overexpression is due to a dominant-interfering effect.

  14. Conserved regulatory modules in the Sox9 testis-specific enhancer predict roles for SOX, TCF/LEF, Forkhead, DMRT, and GATA proteins in vertebrate sex determination.

    Science.gov (United States)

    Bagheri-Fam, Stefan; Sinclair, Andrew H; Koopman, Peter; Harley, Vincent R

    2010-03-01

    While the primary sex determining switch varies between vertebrate species, a key downstream event in testicular development, namely the male-specific up-regulation of Sox9, is conserved. To date, only two sex determining switch genes have been identified, Sry in mammals and the Dmrt1-related gene Dmy (Dmrt1bY) in the medaka fish Oryzias latipes. In mice, Sox9 expression is evidently up-regulated by SRY and maintained by SOX9 both of which directly activate the core 1.3 kb testis-specific enhancer of Sox9 (TESCO). How Sox9 expression is up-regulated and maintained in species without Sry (i.e. non-mammalian species) is not understood. In this study, we have undertaken an in-depth comparative genomics approach and show that TESCO contains an evolutionarily conserved region (ECR) of 180 bp which is present in marsupials, monotremes, birds, reptiles and amphibians. The ECR contains highly conserved modules that predict regulatory roles for SOX, TCF/LEF, Forkhead, DMRT, and GATA proteins in vertebrate sex determination/differentiation. Our data suggest that tetrapods share common aspects of Sox9 regulation in the testis, despite having different sex determining switch mechanisms. They also suggest that Sox9 autoregulation is an ancient mechanism shared by all tetrapods, raising the possibility that in mammals, SRY evolved by mimicking this regulation. The validation of ECR regulatory sequences conserved from human to frogs will provide new insights into vertebrate sex determination. Copyright 2009 Elsevier Ltd. All rights reserved.

  15. Nitrogen-responsive Regulation of GATA Protein Family Activators Gln3 and Gat1 Occurs by Two Distinct Pathways, One Inhibited by Rapamycin and the Other by Methionine Sulfoximine*

    Science.gov (United States)

    Georis, Isabelle; Tate, Jennifer J.; Cooper, Terrance G.; Dubois, Evelyne

    2011-01-01

    Nitrogen availability regulates the transcription of genes required to degrade non-preferentially utilized nitrogen sources by governing the localization and function of transcription activators, Gln3 and Gat1. TorC1 inhibitor, rapamycin (Rap), and glutamine synthetase inhibitor, methionine sulfoximine (Msx), elicit responses grossly similar to those of limiting nitrogen, implicating both glutamine synthesis and TorC1 in the regulation of Gln3 and Gat1. To better understand this regulation, we compared Msx- versus Rap-elicited Gln3 and Gat1 localization, their DNA binding, nitrogen catabolite repression-sensitive gene expression, and the TorC1 pathway phosphatase requirements for these responses. Using this information we queried whether Rap and Msx inhibit sequential steps in a single, linear cascade connecting glutamine availability to Gln3 and Gat1 control as currently accepted or alternatively inhibit steps in two distinct parallel pathways. We find that Rap most strongly elicits nuclear Gat1 localization and expression of genes whose transcription is most Gat1-dependent. Msx, on the other hand, elicits nuclear Gln3 but not Gat1 localization and expression of genes that are most Gln3-dependent. Importantly, Rap-elicited nuclear Gln3 localization is absolutely Sit4-dependent, but that elicited by Msx is not. PP2A, although not always required for nuclear GATA factor localization, is highly required for GATA factor binding to nitrogen-responsive promoters and subsequent transcription irrespective of the gene GATA factor specificities. Collectively, our data support the existence of two different nitrogen-responsive regulatory pathways, one inhibited by Msx and the other by rapamycin. PMID:22039046

  16. Nitrogen-responsive regulation of GATA protein family activators Gln3 and Gat1 occurs by two distinct pathways, one inhibited by rapamycin and the other by methionine sulfoximine.

    Science.gov (United States)

    Georis, Isabelle; Tate, Jennifer J; Cooper, Terrance G; Dubois, Evelyne

    2011-12-30

    Nitrogen availability regulates the transcription of genes required to degrade non-preferentially utilized nitrogen sources by governing the localization and function of transcription activators, Gln3 and Gat1. TorC1 inhibitor, rapamycin (Rap), and glutamine synthetase inhibitor, methionine sulfoximine (Msx), elicit responses grossly similar to those of limiting nitrogen, implicating both glutamine synthesis and TorC1 in the regulation of Gln3 and Gat1. To better understand this regulation, we compared Msx- versus Rap-elicited Gln3 and Gat1 localization, their DNA binding, nitrogen catabolite repression-sensitive gene expression, and the TorC1 pathway phosphatase requirements for these responses. Using this information we queried whether Rap and Msx inhibit sequential steps in a single, linear cascade connecting glutamine availability to Gln3 and Gat1 control as currently accepted or alternatively inhibit steps in two distinct parallel pathways. We find that Rap most strongly elicits nuclear Gat1 localization and expression of genes whose transcription is most Gat1-dependent. Msx, on the other hand, elicits nuclear Gln3 but not Gat1 localization and expression of genes that are most Gln3-dependent. Importantly, Rap-elicited nuclear Gln3 localization is absolutely Sit4-dependent, but that elicited by Msx is not. PP2A, although not always required for nuclear GATA factor localization, is highly required for GATA factor binding to nitrogen-responsive promoters and subsequent transcription irrespective of the gene GATA factor specificities. Collectively, our data support the existence of two different nitrogen-responsive regulatory pathways, one inhibited by Msx and the other by rapamycin.

  17. PTEN differentially regulates expressions of ICAM-1 and VCAM-1 through PI3K/Akt/GSK-3β/GATA-6 signaling pathways in TNF-α-activated human endothelial cells.

    Science.gov (United States)

    Tsoyi, Konstantin; Jang, Hwa Jin; Nizamutdinova, Irina Tsoy; Park, Kyungok; Kim, Young Min; Kim, Hye Jung; Seo, Han Geuk; Lee, Jae Heun; Chang, Ki Churl

    2010-11-01

    Phosphotase and tensin homolog deleted on chromosome 10 (PTEN) is a potent negative regulator of PI3K/Akt pathway. Here, we tried to elucidate the role of PTEN in the regulation of endothelial adhesion molecules, vascular cell adhesion molecule (VCAM)-1 and intracellular adhesion molecule (ICAM)-1, induced by TNF-α in human endothelial cells (ECs). Transfection with PTEN overexpressing vector resulted in the significant decrease in phosphorylation of Akt in TNF-α-treated ECs. PTEN strongly inhibited VCAM-1 but not ICAM-1, however this inhibitory effect was reversed by co-transfection with constitutively active-Akt (CA-Akt-HA) in TNF-α-stimulated ECs. Additionally, silencing of PTEN with specific siRNA showed significant increase of phosphor-Akt compared with TNF-α alone treated ECs. siPTEN significantly upregulated VCAM-1 but was indifferent to ICAM-1 in TNF-α-treated cells. Further, chromatin immunoprecipitation (ChIP) assay showed that PTEN targets GATA-6 but not IRF-1 binding to VCAM-1 promoter. In addition, GATA-6 is associated with glycogen synthesis kinase-3beta (GSK-3β) which is in turn regulated by PTEN-dependent Akt activity. Finally, PTEN significantly prevented monocyte adhesion to TNF-α-induced ECs probably through VCAM-1 regulation. It is concluded that PTEN selectively inhibits expression of VCAM-1 but not ICAM-1 through modulation of PI3K/Akt/GSK-3β/GATA-6 signaling cascade in TNF-α-treated ECs. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

  18. Johne's disease in cattle is associated with enhanced expression of genes encoding IL-5, GATA-3, tissue inhibitors of matrix metalloproteinases 1 and 2, and factors promoting apoptosis in peripheral blood mononuclear cells

    DEFF Research Database (Denmark)

    Coussens, P.M.; Pudrith, C.B.; Skovgaard, Kerstin

    2005-01-01

    remodeling deficiencies through higher expression of tissue inhibitor of matrix metalloproteinase (TIMP) 1 and TIMP2 RNA and lower expression of matrix metalloproteinase (MMP) 14 RNA than similar cells from healthy controls, and that cells within the PBMC population of M. paratuberculosis-infected cows...... upon by quantitative real-time PCR (Q-RT-PCR). Our results indicate that T cells within PBMCs from M. paratuberculosis-infected cows have adopted a predominant Th 2-like phenotype (enhanced expression of IL-5, GATA 3, and possibly IL-4 mRNA), that cells within infected cow PBMCs may exhibit tissue...

  19. Caudal brain infarctions in a kitten – case reportInfartos em região encefálica caudal em gata filhote – relato de caso

    Directory of Open Access Journals (Sweden)

    Lucas Alécio Gomes

    2013-05-01

    Full Text Available Stroke is uncommon in animals compared with humans because of the lower incidence of atherosclerosis and primary hypertension. However with advanced imaging, vascular disease is being recognized with increasing frequency in veterinary medicine. Cerebrovascular disease can be subdivided into infarction and hemorrhage, although the two categories overlap in the case of hemorrhagic infarcts. The aim of thisarticle is to report the neurological manifestations associated with stroke (infarctions in at two-month old, domestic shorthair cat. Neurological evaluation revealed head tilt, tetraparesis, proprioceptive deficits in all four limbs, and decreased pupillary light reflex. Further, manifestations of neurological dysfunctions were acute and progressive. At the necropsy, grossly there were hemorrhage and necrosis at mid-brain and cerebellum. Histopathology confirmed liquefactive necrosis at the mid-brain and cerebellum. The neurological manifestations associated with the pathological findings are suggestive of an anoxic infarction probably due to vascular occlusion. Em animais é baixa a incidência de arterosclerose e hipertensão primária. Devido a tal característica, infarto cerebral é incomum nos mesmos. Entretanto, com o avanço das modalidades de imagem, doença vascular está sendo reconhecida com maio frequência na medicina veterinária. Doença cerebrovascular pode ser subdividida em infarto e hemorragia, embora as duas categorias se interponham no caso de infartos hemorrágicos. Assim sendo, o objetivo deste artigo é descrever as manifestações neurológicas associadas a acidente vascular (infartos em uma gata de dois meses de idade, sem raça definida e domiciliada. Na avaliação neurológica observou-se inclinação de cabeça, tetraparesia, déficits proprioceptivos nos quatro membros e diminuição do reflexo pupilar a luz. Além disso, os problemas neurológicos foram agudos e progressivos. Na necropsia macroscopicamente

  20. Helicase-like transcription factor (Hltf regulates G2/M transition, Wt1/Gata4/Hif-1a cardiac transcription networks, and collagen biogenesis.

    Directory of Open Access Journals (Sweden)

    Rebecca A Helmer

    Full Text Available HLTF/Hltf regulates transcription, remodels chromatin, and coordinates DNA damage repair. Hltf is expressed in mouse brain and heart during embryonic and postnatal development. Silencing Hltf is semilethal. Seventy-four percent of congenic C57BL/6J Hltf knockout mice died, 75% within 12-24 hours of birth. Previous studies in neonatal (6-8 hour postpartum brain revealed silencing Hltf disrupted cell cycle progression, and attenuated DNA damage repair. An RNA-Seq snapshot of neonatal heart transcriptome showed 1,536 of 20,000 total transcripts were altered (p < 0.05 - 10 up- and 1,526 downregulated. Pathway enrichment analysis with MetaCore™ showed Hltf's regulation of the G2/M transition (p=9.726E(-15 of the cell cycle in heart is nearly identical to its role in brain. In addition, Brca1 and 12 members of the Brca1 associated genome surveillance complex are also downregulated. Activation of caspase 3 coincides with transcriptional repression of Bcl-2. Hltf loss caused downregulation of Wt1/Gata4/Hif-1a signaling cascades as well as Myh7b/miR499 transcription. Hltf-specific binding to promoters and/or regulatory regions of these genes was authenticated by ChIP-PCR. Hif-1a targets for prolyl (P4ha1, P4ha2 and lysyl (Plod2 collagen hydroxylation, PPIase enzymes (Ppid, Ppif, Ppil3 for collagen trimerization, and lysyl oxidase (Loxl2 for collagen-elastin crosslinking were downregulated. However, transcription of genes for collagens, fibronectin, Mmps and their inhibitors (Timps was unaffected. The collective downregulation of genes whose protein products control collagen biogenesis caused disorganization of the interstitial and perivascular myocardial collagen fibrillar network as viewed with picrosirius red-staining, and authenticated with spectral imaging. Wavy collagen bundles in control hearts contrasted with collagen fibers that were thin, short and disorganized in Hltf null hearts. Collagen bundles in Hltf null hearts were tangled and

  1. FY 2001 report on the survey of the formation promotion subsidy project on the environmentally friendly type energy community. Potential survey of the introduction of a processing/power generation system using ASR in the South region of Tochigi Prefecture; 2001 nendo kankyo chowa gata energy komyuniti keisei sokushin hojo jigyo. Tochigi ken nanbu chiiki ni okeru ASR (Shuredda dust) wo nenryo to shita shori hatsuden system no donyu kanosei chosa chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For the consistent processing of waste automobile in Tochigi Prefecture and the neighboring area, survey/study were made of the thermal recycle using shredder dust, ASR (automobile shredder residue), as fuel as well as the material recycle. As to the amount of material recycle of waste automobile, the amount of potential recycle of iron, copper, plastic, etc. was estimated, and it was found out that the potential recycle of parts such as engine was 40-45%, that of iron scrap 35-40%, and that of ASR 20-25%. The amount of fuel (ASR amount) to be thrown into the gasification power generation system was set up at 150t. As the gasification power generation system, the pyrolysis gasifier and shaft kiln gasification melting furnace were selected. In the study of economical efficiency of the gasification power generation system, the following were considered: ASR processing price, plant cost, subsidy, running cost, char receiving price, power selling price, etc., but even in the case of the pyrolysis gasifier with highest valuation, both payback years and IRR did not reach the target. (NEDO)

  2. Fiscal 2000 achievement report on the venture business assisting type regional consortium - Core industry creation type. Research and development of energy conservation oriented collective detoxification system for dioxins and toxic heavy metals in fly ash and soil; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo seika hokokusho. Hibai dojochu no dioxin rui yugai jukinzoku no sho energy gata ikkatsu mugaika shori system ni kansuru kenkyu kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Efforts are made to develop a system to collectively detoxify dioxins and heavy metals in incinerator fly ash or in polluted soil using microbes and/or chemicals. In the use of chemicals, verification tests are conducted for a system to detoxify dioxins and toxic heavy metals simultaneously. In the use of microbes, an effort is made to develop a low cost energy conservation type treatment system based on what is provided by the study of microbes such as Phanerochaete chrysosporium. As for the treatment of fly ash, an effort is made to recycle it into resources usable in the development of marketable products. For the detoxification of dioxins using chemicals, they are dechlorinated in a chemical reduction reaction. For their detoxification with help of microbes, it is found in an experiment of ash fly dioxin decomposition using Phanerochaete chrysosporium that the microbe reduces dioxins in both liquid phase and solid phase systems. The result of verification tests using chemicals shows that the reference level of 3ng-TEQ/g is easily cleared in the treatment of high concentration ash fly. In the detoxification of heavy metals, data is obtained indicating that the elution level of lead in ash fly is found reduced by 70-85% after treatment. (NEDO)

  3. Effects of β-D-mannuronic acid, as a novel non-steroidal anti-inflammatory medication within immunosuppressive properties, on IL17, RORγt, IL4 and GATA3 gene expressions in rheumatoid arthritis patients

    Directory of Open Access Journals (Sweden)

    Barati A

    2017-03-01

    Full Text Available Anis Barati,1 Ahmad Reza Jamshidi,2,* Hossein Ahmadi,1 Zahra Aghazadeh,1 Abbas Mirshafiey1,* 1Department of Immunology, School of Public Health, 2Iranian Institute for Health Sciences Research, Rheumatology Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran *These authors contributed equally to this work Abstract: Rheumatoid arthritis (RA is the most common form of chronic inflammatory arthritis characterized by pain, swelling and destruction of joints, with a resultant disability. Disease-modifying anti-rheumatic drugs (DMARDs and biological drugs can interfere with the disease process. In this study, the effect of β-D-mannuronic acid (M2000 as a novel non-steroidal anti-inflammatory drug (NSAID with immunosuppressive and anti-inflammatory effects together with antioxidant effects was evaluated on IL17, RORγt, IL4 and GATA3 gene expression in 12 RA patients. Previously, M2000 driven from sodium alginate (natural product; patented, DEU: 102016113018.4 has shown a notable efficacy in experimental models of multiple sclerosis, RA and nephrotic syndrome. This study was performed on 12 patients with RA who had an inadequate response to conventional treatments. During this trial, patients were permitted to continue the conventional therapy excluding NSAIDs. M2000 was administered orally at a dose of 500 mg twice daily for 12 weeks. The peripheral blood mononuclear cells (PBMCs were collected before and after treatment to evaluate the expression levels of IL4, GATA3, IL17 and RORγt. The gene expression results showed that M2000 has a potent efficacy, so that it could not only significantly decrease IL17 and RORγt levels but also increase IL4 and GATA3 levels after 12 weeks of treatment. Moreover, the gene expression results were in accordance with the clinical and preclinical assessments. In conclusion, M2000 as a natural novel agent has therapeutic and immunosuppressive properties on RA patients (identifier

  4. HEMANGIOMA HEPÁTICO PRIMÁRIO EM GATA PERSA COM DOENÇA RENAL POLICÍSTICA PRIMARY HEPATIC HEMANGIOMA IN PERSIAN CAT WITH POLYCYSTIC KIDNEY DISEASE

    Directory of Open Access Journals (Sweden)

    Valdemiro Amaro da Silva Júnior

    2008-07-01

    Full Text Available

    Hemangiomas primários do fígado são neoplasias benignas que podem comprometer a sobrevivência do animal, em virtude da compressão de vasos, ductos e hepatócitos adjacentes, especialmente pela possibilidade de rupturas e morte por hipovolemia. Por serem raros em felinos, objetiva-se relatar o caso de uma gata Persa, com 10 anos de idade, cujos sinais clínicos eram: aumento de volume abdominal, vômitos intermitentes, apatia e anorexia, além de cios irregulares. No exame radiográfico se observou massa tecidual radiopaca no fígado. Na ultra-sonografia hepática, percebiam-se contornos irregulares, parênquima heterogêneo, hiperecogênico, com áreas cavitárias, sugestivo de neoplasia e cistos. No exame macroscópico, visibilizaram-se ascite sanguinolenta, esteatose hepática e massa neoplásica única com 12 cm de comprimento por 8 cm de largura e diversos cistos. Os ovários e rins se apresentavam policísticos e havia hiperplasia endometrial cística. Microscopicamente foram diagnosticadas no fígado formações císticas limitadas por endotélio e delgada cápsula de tecido conjuntivo, esteatose e hepatite periportal mononuclear-linfocitária com proliferação de ductos bilíferos. A massa tumoral originava-se a partir do tecido conjuntivo da cápsula hepática e se caracterizava pela presença de brotos vasculares oriundos de células endoteliais, com dilatação e anastomose de vasos nas áreas mais superficiais. Os achados observados são compatíveis com o diagnóstico de hemangioma hepático primário do tipo capilar-cavernoso. Nos ovários, no útero, nos rins e fígado, constatou-se doença policística.

     

    PALAVRAS-CHAVES: Fígado, gato, neoplasia vascular.

    Primaries hepatics hemangiomas are benign tumors that may compel limitations to animal survival owing to the compression of blood vessels, ducts and contiguous hepatocytes, especially due a

  5. Respostas cardiorrespiratória e metabólica do propofol nas formulações em emulsão lipídica ou microemulsão em gatas

    Directory of Open Access Journals (Sweden)

    Renato Batista Tamanho

    2013-08-01

    Full Text Available O presente estudo objetiva avaliar os efeitos cardiorrespiratórios e metabólicos do propofol em emulsão lipídica e microemulsão em gatas. Foram utilizadas 12 gatas, hígidas, adultas, alocadas em dois grupos: microemulsão (MICRO, n=6 e emulsão lipídica (EMU, n=6, os quais receberam propofol, na respectiva formulação, em dose suficiente para intubação. Em seguida, foram intubados, fornecendo-se oxigênio 100%, em sistema sem reinalação de gases. Ato contínuo, iniciou-se a infusão de propofol na dose de 0,3mg kg-1 min-1 durante 90 minutos. A dose necessária para indução foi de 9,5±1,3mg kg-1 e 10±1mg kg-1 para MICRO e EMU, respectivamente. Os valores de pressão arterial sistólica (PAS, pressão arterial média (PAM, pressão arterial diastólica (PAD e pH foram menores, em todos os momentos, no EMU em relação ao MICRO; a f do EMU foi menor de T30 até T75 em relação ao MICRO. A PaCO2 do EMU foi maior de T15 até T90. Os tempos de extubação, decúbito esternal, deambulação e recuperação total foram de 40,6±30,7; 91±37,5; 134,5±54,5 e 169,1±55,4 minutos no MICRO e de 68,8±37,3; 133,3±85,3; 171,3±77,1 e 233,1±60,6 minutos no EMU, respectivamente. Houve aumento da enzima Alanina Aminotransferase de 12 às 72h no EMU e de 48 às 72h no MICRO. O propofol em microemulsão apresentou características clínicas de indução e manutenção, bem como efeitos metabólicos semelhantes à formulação em emulsão lipídica. A formulação em microemulsão proporcionou maior estabilidade cardiovascular e respiratória para indução e infusão contínua em gatas hígidas.

  6. Minocycline Blocks Asthma-associated Inflammation in Part by Interfering with the T Cell Receptor-Nuclear Factor κB-GATA-3-IL-4 Axis without a Prominent Effect on Poly(ADP-ribose) Polymerase*

    Science.gov (United States)

    Naura, Amarjit S.; Kim, Hogyoung; Ju, Jihang; Rodriguez, Paulo C.; Jordan, Joaquin; Catling, Andrew D.; Rezk, Bashir M.; Elmageed, Zakaria Y. Abd; Pyakurel, Kusma; Tarhuni, Abdelmetalab F.; Abughazleh, Mohammad Q.; Errami, Youssef; Zerfaoui, Mourad; Ochoa, Augusto C.; Boulares, A. Hamid

    2013-01-01

    Minocycline protects against asthma independently of its antibiotic function and was recently reported as a potent poly(ADP-ribose) polymerase (PARP) inhibitor. In an animal model of asthma, a single administration of minocycline conferred excellent protection against ovalbumin-induced airway eosinophilia, mucus hypersecretion, and Th2 cytokine production (IL-4/IL-5/IL-12(p70)/IL-13/GM-CSF) and a partial protection against airway hyperresponsiveness. These effects correlated with pronounced reduction in lung and sera allergen-specific IgE. A reduction in poly(ADP-ribose) immunoreactivity in the lungs of minocycline-treated/ovalbumin-challenged mice correlated with decreased oxidative DNA damage. The effect of minocycline on PARP may be indirect, as the drug failed to efficiently block direct PARP activation in lungs of N-methyl-N′-nitro-N-nitroso-guanidine-treated mice or H2O2-treated cells. Minocycline blocked allergen-specific IgE production in B cells potentially by modulating T cell receptor (TCR)-linked IL-4 production at the mRNA level but not through a modulation of the IL-4-JAK-STAT-6 axis, IL-2 production, or NFAT1 activation. Restoration of IL-4, ex vivo, rescued IgE production by minocycline-treated/ovalbumin-stimulated B cells. IL-4 blockade correlated with a preferential inhibition of the NF-κB activation arm of TCR but not GSK3, Src, p38 MAPK, or ERK1/2. Interestingly, the drug promoted a slightly higher Src and ERK1/2 phosphorylation. Inhibition of NF-κB was linked to a complete blockade of TCR-stimulated GATA-3 expression, a pivotal transcription factor for IL-4 expression. Minocycline also reduced TNF-α-mediated NF-κB activation and expression of dependent genes. These results show a potentially broad effect of minocycline but that it may block IgE production in part by modulating TCR function, particularly by inhibiting the signaling pathway, leading to NF-κB activation, GATA-3 expression, and subsequent IL-4 production. PMID:23184953

  7. Minocycline blocks asthma-associated inflammation in part by interfering with the T cell receptor-nuclear factor κB-GATA-3-IL-4 axis without a prominent effect on poly(ADP-ribose) polymerase.

    Science.gov (United States)

    Naura, Amarjit S; Kim, Hogyoung; Ju, Jihang; Rodriguez, Paulo C; Jordan, Joaquin; Catling, Andrew D; Rezk, Bashir M; Abd Elmageed, Zakaria Y; Pyakurel, Kusma; Tarhuni, Abdelmetalab F; Abughazleh, Mohammad Q; Errami, Youssef; Zerfaoui, Mourad; Ochoa, Augusto C; Boulares, A Hamid

    2013-01-18

    Minocycline protects against asthma independently of its antibiotic function and was recently reported as a potent poly(ADP-ribose) polymerase (PARP) inhibitor. In an animal model of asthma, a single administration of minocycline conferred excellent protection against ovalbumin-induced airway eosinophilia, mucus hypersecretion, and Th2 cytokine production (IL-4/IL-5/IL-12(p70)/IL-13/GM-CSF) and a partial protection against airway hyperresponsiveness. These effects correlated with pronounced reduction in lung and sera allergen-specific IgE. A reduction in poly(ADP-ribose) immunoreactivity in the lungs of minocycline-treated/ovalbumin-challenged mice correlated with decreased oxidative DNA damage. The effect of minocycline on PARP may be indirect, as the drug failed to efficiently block direct PARP activation in lungs of N-methyl-N'-nitro-N-nitroso-guanidine-treated mice or H(2)O(2)-treated cells. Minocycline blocked allergen-specific IgE production in B cells potentially by modulating T cell receptor (TCR)-linked IL-4 production at the mRNA level but not through a modulation of the IL-4-JAK-STAT-6 axis, IL-2 production, or NFAT1 activation. Restoration of IL-4, ex vivo, rescued IgE production by minocycline-treated/ovalbumin-stimulated B cells. IL-4 blockade correlated with a preferential inhibition of the NF-κB activation arm of TCR but not GSK3, Src, p38 MAPK, or ERK1/2. Interestingly, the drug promoted a slightly higher Src and ERK1/2 phosphorylation. Inhibition of NF-κB was linked to a complete blockade of TCR-stimulated GATA-3 expression, a pivotal transcription factor for IL-4 expression. Minocycline also reduced TNF-α-mediated NF-κB activation and expression of dependent genes. These results show a potentially broad effect of minocycline but that it may block IgE production in part by modulating TCR function, particularly by inhibiting the signaling pathway, leading to NF-κB activation, GATA-3 expression, and subsequent IL-4 production.

  8. Expression of alpha V integrin is modulated by Epstein-Barr virus nuclear antigen 3C and the metastasis suppressor Nm23-H1 through interaction with the GATA-1 and Sp1 transcription factors

    International Nuclear Information System (INIS)

    Choudhuri, Tathagata; Verma, Subhash C.; Lan, Ke; Robertson, Erle S.

    2006-01-01

    Epstein-Barr virus (EBV) is a lymphotrophic herpesvirus infecting most of the world's population. It is associated with a number of human lymphoid and epithelial tumors and lymphoproliferative diseases in immunocompromised patients. A subset of latent EBV antigens is required for immortalization of primary B-lymphocytes. The metastatic suppressor Nm23-H1 which is downregulated in human invasive breast carcinoma reduces the migration and metastatic activity of breast carcinoma cells when expressed from a heterologous promoter. Interestingly, the EBV nuclear antigen 3C (EBNA3C) reverses these activities of Nm23-H1. The alpha V integrins recognize a variety of ligands for signaling and are involved in cell migration and proliferation and also serve as major receptors for extracellular-matrix-mediated cell adhesion and migration. The goal of this study was to determine if Nm23-H1 and EBNA3C can modulate alpha V integrin expression and downstream activities. The results of our studies indicate that Nm23-H1 downregulates alpha V intregrin expression in a dose responsive manner. In contrast, EBNA3C can upregulate alpha V integrin expression. Furthermore, the study showed that the association of the Sp1 and GATA transcription factors with Nm23-H1 is required for modulation of the alpha V integrin activity. Thus, these results suggest a direct correlation between the alpha V integrin expression and the interaction of Nm23-H1 with EBNA3C

  9. Myocardial Gene Expression of T-bet, GATA-3, Ror-γt, FoxP3, and Hallmark Cytokines in Chronic Chagas Disease Cardiomyopathy: An Essentially Unopposed TH1-Type Response

    Directory of Open Access Journals (Sweden)

    Luciana Gabriel Nogueira

    2014-01-01

    Full Text Available Background. Chronic Chagas disease cardiomyopathy (CCC, a late consequence of Trypanosoma cruzi infection, is an inflammatory cardiomyopathy with prognosis worse than those of noninflammatory etiology (NIC. Although the T cell-rich myocarditis is known to play a pathogenetic role, the relative contribution of each of the functional T cell subsets has never been thoroughly investigated. We therefore assessed gene expression of cytokines and transcription factors involved in differentiation and effector function of each functional T cell subset (TH1/TH2/TH17/Treg in CCC, NIC, and heart donor myocardial samples. Methods and Results. Quantitative PCR showed markedly upregulated expression of IFN-γ and transcription factor T-bet, and minor increases of GATA-3; FoxP3 and CTLA-4; IL-17 and IL-18 in CCC as compared with NIC samples. Conversely, cytokines expressed by TH2 cells (IL-4, IL-5, and IL-13 or associated with Treg (TGF-β and IL-10 were not upregulated in CCC myocardium. Expression of TH1-related genes such as T-bet, IFN-γ, and IL-18 correlated with ventricular dilation, FoxP3, and CTLA-4. Conclusions. Results are consistent with a strong local TH1-mediated response in most samples, possibly associated with pathological myocardial remodeling, and a proportionally smaller FoxP3+CTLA4+ Treg cell population, which is unable to completely curb IFN-γ production in CCC myocardium, therefore fueling inflammation.

  10. DESCOLORIMENTO DE EFLUENTE ASSOCIADO AO TINGIMENTO DE ÁGATAS CONTENDO RODAMINA B COM DIFERENTES COMBINAÇÕES FOTOCATALÍTICAS VIA PROCESSOS OXIDATIVOS AVANÇADOS (POA´s

    Directory of Open Access Journals (Sweden)

    Tiago Bender Wermuth

    2013-12-01

    Full Text Available O tingimento é um dos processos de acabamento das pedras semi-preciosas até a sua comercialização. Este é muito usado na indústria da ágata utilizando elevada demanda de água o que gera uma grande quantidade de águas residuárias, contendo moléculas corantes complexas, como a Rodamina B, que apresenta características de poluente orgânico com ecotoxicidade e genotoxicidade elevadas. Neste trabalho objetivou-se buscar o desenvolvimento de POA’s capazes de degradar os efluentes sintéticos provenientes do tingimento de pedras semi-preciosas contendo o corante Rodamina B (C.I 45170. Para uma melhor eficiência de remoção da Rodamina B, utilizou-se dois métodos distintos de fixação do catalisador utilizado. Primeiramente se trabalho com sílica-gel branca (1-3 mm, Tyvek e posteriormente com o Polietileno de baixa densidade (PEBD. Realizada a preparação dos materiais, investigou-se algumas combinações de métodos como UV/TiO2/SiO2/O3, UV/TiO2/PEBD/O3 bem como, o monitoramento de alguns parâmetros, como pH e a absorbância de amostras sintéticas. Os testes foram realizados em um reator em acrílico composto de lâmpada germicida (SCT – G13/T8/15W, onde se trabalhou com um volume útil de 1 L.Compondo ainda o reator, utilizou-se uma proveta de 1 litro e uma bomba peristáltica. A concentração de Rodamina B utilizada foi de 20 mg L-1. A partir dos resultados obtidos foi possível verificar que a combinação do UV/TiO2/PEBD/O3 mostrou-se ser mais eficiente. Essa combinação proporcionou um valor de constante cinética na ordem de 28,6 x 10-3 min-1. Se comparado aos demais processos investigados, pode-se observar que se teve um aumento do valor de k.

  11. Leucogram and serum acute phase protein concentrations in queens submitted to conventional or videolaparoscopic ovariectomy Leucograma e concentrações séricas de proteínas de fase aguda de gatas submetidas à ovariectomia convencional ou por videolaparoscopia

    Directory of Open Access Journals (Sweden)

    A.E. Alves

    2010-02-01

    Full Text Available Thirty health queens were submitted to ovariectomy by conventional technique or by videolaparoscopy. In order to study the intensity of inflammatory response by means of acute phase protein analysis and white blood cell count, serum samples were taken before and until 144 hours after the surgical procedures. The protein concentrations that were significantly increased 24 hours after surgical procedures were: ceruloplasmin, hemopexin, haptoglobin, and α1-acid glycoprotein, 69.8%, 103.5%, 117.3%, and 199.0%, respectively, for conventional ovariectomy; and 22.3%, 46.1%, 79.8%, and 74.6%, respectively, for laparoscopic ovariectomy. Therefore, inflammatory response was more intense in queens submitted to conventional ovariectomy. Results indicate that the increase or decrease in acute phase proteins, as well as in white blood cells count, may be useful in the evaluation of inflammatory response induced by these surgical procedures.Trinta gatas, saudáveis, foram submetidas à ovariectomia pela técnica convencional e por videolaparoscopia. Amostras de sangue foram obtidas com o objetivo de verificar a intensidade da resposta inflamatória por meio da análise das concentrações de proteinas de fase aguda e contagem de leucócitos antes e até 144 horas após procedimento cirúrgico. As proteínas que apresentaram aumento significativo 24 horas após a cirurgia foram: ceruloplasmina, hemopexina, haptoglobina e α1-glicoproteína ácida, 69,8%, 103,5%, 117,3% e 199,0%, respectivamente, para ovariectomia convencional, e 22,3%, 46,1%, 79,8% e 74,6%, respectivamente, para ovariectomia por videolaparoscopia. A resposta inflamatória foi mais evidente nas gatas submetidas à ovariectomia convencional. Os resultados mostram aumento e diminuição na concentração de proteínas de fase aguda e na contagem de leucócitos, podendo ser utilizados na avaliação da resposta inflamatória induzida por procedimentos cirúrgicos.

  12. Bucket elevator type continuous unloader; Basket elevator gata renzokushiki anroda

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-04-20

    This unloader was delivered to Wakayama branch of Sumikin Butsuryu in the site of Wakayama steel plant of Sumitomo Metal Industries Ltd. for landing sub-materials for ironworks. Main specifications: (1) Capacity: 400t/h for limestone, 280t/h for gypsum, (2) Kinds of ships: 5,000DWT maximum, (3) Boom swing radius: 20m. Features: (1) Simple light-weight unloader for not large ships for main materials but small coastal service ships which works with not swing motion but only traveling and normal-directional motion of the under structure of a bucket elevator unit, (2) Various useful functions such as ground conveyance, truck loading, back yard stacking and back yard shipment for handling of various sub-materials. (translated by NEDO)

  13. Role of GATA Transcription Factors in the T Cell Lineage

    NARCIS (Netherlands)

    J.P. van Hamburg (Jan Piet)

    2008-01-01

    textabstractT lymphocytes play a central role in the mammalian immune response against potentially hazardous pathogens, such as parasites, bacteria, viruses and fungi. These cells have the remarkable capacity to specifically recognize foreign substances, termed antigens, to which they respond by

  14. GATA: A graphic alignment tool for comparative sequenceanalysis

    Energy Technology Data Exchange (ETDEWEB)

    Nix, David A.; Eisen, Michael B.

    2005-01-01

    Several problems exist with current methods used to align DNA sequences for comparative sequence analysis. Most dynamic programming algorithms assume that conserved sequence elements are collinear. This assumption appears valid when comparing orthologous protein coding sequences. Functional constraints on proteins provide strong selective pressure against sequence inversions, and minimize sequence duplications and feature shuffling. For non-coding sequences this collinearity assumption is often invalid. For example, enhancers contain clusters of transcription factor binding sites that change in number, orientation, and spacing during evolution yet the enhancer retains its activity. Dotplot analysis is often used to estimate non-coding sequence relatedness. Yet dot plots do not actually align sequences and thus cannot account well for base insertions or deletions. Moreover, they lack an adequate statistical framework for comparing sequence relatedness and are limited to pairwise comparisons. Lastly, dot plots and dynamic programming text outputs fail to provide an intuitive means for visualizing DNA alignments.

  15. 2D resistivity survey in complex geological structure area. Application to the volcanic area; Fukuzatsuna chishitsu kozo chiiki ni okeru hiteiko nijigen tansa. Kazangan chiiki deno tekiorei

    Energy Technology Data Exchange (ETDEWEB)

    Asakawa, S; Ikuma, T; Tanifuji, R [DIA Consultants Co. Ltd., Tokyo (Japan)

    1996-05-01

    Introduced herein is an application of 2D resistivity survey to a volcanic rock area where the survey result is difficult to interpret because of its complex geological structure. In a dam site survey, main problems involve the permeability of water through faults and weathered, altered zones. At this site, a 2D resistivity survey was conducted, a 2D geological structure was deduced from the resistivity section, and the result was examined. It was found that resistivity distribution was closely related to hydrographic factors, but no obvious correlation was detected between rock classes and R, Q, and D. In conducting investigations into a section planned for a highway tunnel, it was learned that the problem was a volcanic ash layer to collapse instantly upon absorbing water, and the distribution of the ash layer, not to be disclosed by boring, was subjected to a 2D resistivity survey. The survey was conducted into the structure above where the tunnel would run, and further into the face, and studies were made about what layer was reflected by the resistivity distribution obtained by analysis. The result of the analysis agreed with the details of the layer that was disclosed afterward. 4 figs., 1 tab.

  16. Regional new energy vision for Soryo Town, Hiroshima Prefecture; 2001 nendo Soryo cho chiiki shin energy vision. Chiiki shin energy vision sakutei tou jigyo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    A regional new energy vision was formulated for the town for 'contributing to the solution of environmental problems' and 'contributing to the reenergization of the regional community through utilizing resources in the region.' The final energy consumption of the town is 103,315 times 10{sup 3} MJ, with the transportation sector accounting for a percentage higher than the nation-wide average. The town is characterized in that it has but a small number of manufacturers, offices, or stores. As for new energy, it was estimated that 9117 times 10{sup 3} MJ/year would be gained by methanol produced from cultured weed, 5696 times 10{sup 3} MJ/year by medium/small-scale hydropower generation, 5,233 times 10{sup 3} MJ/year by photovoltaic power generation, and so forth. New energy introduction projects were discussed, which included medium/small-scale hydropower generation at a counter dam; the introduction of photovoltaic power into principal public facilities, general households, and agricultural facilities; introduction of solar heat appliances into principal public facilities and general households; introduction of clean energy automobiles into the town office and general households; and the introduction of pellet-fired stoves into principal public facilities and general households. (NEDO)

  17. Report on new energy vision in Koushida Town area; Koushidacho chiiki shin energy vision hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    A new energy vision was established in the Koushida Town area in Miyagi Prefecture. The population of Koushida Town has leveled off in recent years at 20,245 persons in fiscal 2000. However, number of people working outside the town is increasing, and the ratio of the daytime population is decreasing slowly. Agriculture in this town depends mainly on production rice of such brands as 'Hitomebore' and 'Sasanishiki', wherein the agricultural production amount is 3 billion 260 million yen in fiscal 1998. Commercial activities are decreasing its power with the annual retail sales amount of 17 billion 800 million yen, while the product shipping amount from the attracted factories is on the increasing trend, at 25 billion 700 million yen in 1998. The energy consumption is 526 times 10{sup 9} kcal in total, accounted for by the business department at 42.0%, the transportation department at 32.3%, and the consumer department at 25.7%. The annual utilizable quantity of new energies amounts to 28,816 times 10{sup 6} kcal, made available from solar power, wind power, bio-mass, and refuses, which is about 5.5% of the final energy consumption of the town. The new energy introducing project includes introduction of photovoltaic power generation into the town office building, facilities for interchange of citizens and schools, conversion of the automobiles used in the official organizations into clean energy fueled automobiles, the Eco-town Plan for the east side of Koushida Station, and the project of utilizing bio-mass supplied from the livestock market. (NEDO)

  18. New energy visions for the Town of Ichinohe district; Ichinohecho chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    The new energy visions are drawn for Town of Ichinohe, Iwate Pref. The town's population has been generally decreasing since 1975, from 21,433 to 17,906 in 1995. The town consumed energy totaling 288,691Gcal in 1998, of which the transportation sector accounts for 55%, followed by the domestic, commercial and industrial sectors, in this order, the industrial sector accounting for only 7%. The estimated new energy sources available to the town are wind power, power generated by temperature difference at rivers or using snow, solar energy, biomass energy and energy from wastes, in the order of quantity, totaling 137GWh/y as electrical power and 45.6Tcal/y as heat. The priority projects include making Ichinohe Primary School an eco-school, construction of a biomass demonstration plant in the Okunakayama district, introduction of a boiler firing wood chips at Okunakayama Heights, construction of a wind power mill at Takamori Heights, and introduction of new energies by the citizens. (NEDO)

  19. Report on new energy vision in Koushida Town area; Koushidacho chiiki shin energy vision hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    A new energy vision was established in the Koushida Town area in Miyagi Prefecture. The population of Koushida Town has leveled off in recent years at 20,245 persons in fiscal 2000. However, number of people working outside the town is increasing, and the ratio of the daytime population is decreasing slowly. Agriculture in this town depends mainly on production rice of such brands as 'Hitomebore' and 'Sasanishiki', wherein the agricultural production amount is 3 billion 260 million yen in fiscal 1998. Commercial activities are decreasing its power with the annual retail sales amount of 17 billion 800 million yen, while the product shipping amount from the attracted factories is on the increasing trend, at 25 billion 700 million yen in 1998. The energy consumption is 526 times 10{sup 9} kcal in total, accounted for by the business department at 42.0%, the transportation department at 32.3%, and the consumer department at 25.7%. The annual utilizable quantity of new energies amounts to 28,816 times 10{sup 6} kcal, made available from solar power, wind power, bio-mass, and refuses, which is about 5.5% of the final energy consumption of the town. The new energy introducing project includes introduction of photovoltaic power generation into the town office building, facilities for interchange of citizens and schools, conversion of the automobiles used in the official organizations into clean energy fueled automobiles, the Eco-town Plan for the east side of Koushida Station, and the project of utilizing bio-mass supplied from the livestock market. (NEDO)

  20. New energy introduction guidebook for Chugoku area; Shin energy donyu guidebook. Chugoku chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-05

    A guidebook was edited for the purpose of assisting introduction of new energies. Sections consist of Section 1, new energies available in the Chugoku area; Section 2, procedure for new energy introduction; Section 3, assistance institutions by energy; and Section 4, the status of new energy introduction in the Chugoku area, the future introduction targets, and referential materials. Section 1, upon defining the new energies, summarized features of photovoltaic power generation, solar heat utilization, wind power generation, wastes power generation, cogeneration, fuel cells, clean energy fueled automobiles, and introduction records up to the year 2000. Section 2 summarizes the general roles of local governments in the introduction, processing procedures for individual programs, related regulations, and summary of the assistance institutions. Section 3 puts into order the assistance institutions by energy. Section 4 summarizes precautions, targets and records in introduction by each new energy. The referential materials include a list of the Japanese assistance institutions, and subsidy institutions related to new energies for local governments in the Chugoku area. (NEDO)

  1. New energy vision in Daito Town area; Daitocho chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A new energy vision was established in the Daito Township area in Iwate Prefecture. The energy consumed by Daito town as a whole in fiscal 1998 is about 213,685 two-hundred-kL drums, accounted for by the consumer department at 35.7%, the transportation department at 33.3%, and the industrial department at 31.0%. Carbon dioxide emission amount is estimated to be 109,647 t-CO2. General households in the town consume fossil fuel largely for room heating, whose energy conservation or substitution by new energies is important. The utilizable quantity of new energies in the town include solar energy, forest bio-mass, agricultural resource bio-mass, livestock excreta bio-mass, and refuses, whose total quantity is estimated to be 23,161 drums. The new energy utilization project includes enlightenment activities by introducing a photovoltaic power generator and a small wind power generator into Daito Middle School and the Murone Plateau Natural Energy Park, introduction of solar heat generated hot water supply devices for public facilities and town-operated houses, a discussion on a pellet production line in the wood processing industrial complex, and introduction of pellet boilers into public facilities. (NEDO)

  2. Feasibility study of Copahue geothermal development project, Argentina; Aruzenchin kyowakoku Copahue chiiki no chinetsu kaihatsu chosa

    Energy Technology Data Exchange (ETDEWEB)

    Abe, M.; Yamada, M.; Nakanishi, S.; Todaka, N. [Electric Power Development Co. Ltd., Tokyo (Japan); Fujita, T.

    1996-03-15

    Geothermal resources have been evaluated in the Copahue district. For this survey administrated by JICA, drilling of a deep test well with a small bore (COP-3) and blowout tests were conducted. This district is located at the east side of the Andes elongated in the N-S direction. Volcanoes with similar active periods and active states are distributed in the N-S direction, which forms a divide with the Pacific Ocean side. The Copahue-Caviahue composite volcano in the surveyed area is located nearly in the central part of the volcanic zone, which forms a special ring topography. Geology of the Copahue district consists of volcanic effusive rocks during the Tertiary and Quaternary periods. There are five prospecting fields with geothermal indications in the surveyed area. From the survey, an area of about 13 km{sup 2} was evaluated as a geothermal resource field. Especially, in the area of about 4 km{sup 2} including COP-1, 2 and 3 wells, the steam predominant type reservoirs were found out. As a result of estimation of the geothermal resource potential by the volumetric method, a highly prospective power generation potential of 30 MW was evaluated in the field where the steam predominant type reservoirs were confirmed by the boring wells. 7 refs., 13 figs., 1 tab.

  3. New energy vision of the Gotsu city area; 2001 nendo Gotsu shi chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Gotsu City, Shimane Prefecture, an investigational study was conducted of the energy consumption amount of the city, existence amount of new energy, project for new energy introduction, etc., and a new energy vision was worked out. The energy consumption amount of the city was estimated at 1,558,022 Gcal. By sector, the energy consumption amount consisted of 80.0% in the industrial sector, 11.2% of in the transportation sector, 5.8% in the residential sector and 3.0% in the business sector. In the industrial sector, the main energy consumption industries were the paper manufacturing industry and tile industry. As the project for new energy introduction, the following were studied: project for the wind farm where a lot of wind power generators are installed; park as a symbol having 1 or 2 wind power generators; introduction of solar energy use water warmer/photovoltaic power generation to public facilities; system for promotion of the introduction of solar energy use water warmer/photovoltaic power generation to households; project on CO2 reduction by installation of LNG station; refuse/waste power generation project; project on utilization of the waste heat from tile makers. (NEDO)

  4. Performance analysis in Japanese field test program; Field test data kara no chiiki hatsuden kaiseki hoho

    Energy Technology Data Exchange (ETDEWEB)

    Sugiyama, H.; Kurokawa, K.; Uchida, D. [Tokyo University of Agriculture and Technology, Tokyo (Japan); Otani, K.; Sakuta, K.; Tsuda, I. [Electrotechnical Laboratory, Tsukuba (Japan); Oshiro, T.; Sakamoto, K. [Japan Quality Assurance Organization, Tokyo (Japan)

    1997-11-25

    Power generation characteristics are investigated using data collected in photovoltaic power generation field tests being undertaken by NEDO (New Energy and Industrial Technology Development Organization). The photovoltaic power generation system performance is evaluated by examining loss factors leading to degradation in system performance, with weather conditions such as variation in insolation and air temperature, inverter performance, and shadows cast by surrounding buildings taken into consideration. As the result, it is found that the important loss factors are degradation in module performance due to elevated temperature, drift in the maximum output control, degraded inverter performance due to input power variation, effect of shadows, etc. It is learned that system is greatly affected by degradation in module performance due to increased temperature in summer and by shadows in winter, the two being responsible for the output coefficient dropping to approximately 75% throughout the year. The output coefficient frequency distribution charts for the 75 test sites confirm that the rate is as low as 70-80% at many sites. As for the system operating time, it tends to be longer in West Japan where the annual insolation rate is higher. 3 refs., 10 figs.

  5. New energy vision in Gamagori City area (Aichi Prefecture); Gamagorishi chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    With an objective of early realization of a district society harmonized with the natural environment, a vision was established as a guideline to achieve systematic and effective introduction of new energies, with cooperation from citizens, business entities, and the administration. The activity achievements were put into order by the following six items: 1) movements in introducing new energy systems, 2) identification of the characteristics of the district and major relevant measures, 3) identification of the current status and characteristics of the energy demand structure in the district, 4) estimation of existence and utilizable quantity of new energies, 5) needs of introduction of new energies in the area, and 6) introduction possibilities, realization measures, and proliferation acceleration measures. Item 6 is composed of considerations given from the result of the initial stage investigation, basic ideas, targets of the introduction, introduction policies, and promotion of energy countermeasures. Plans were made on a solar heat hot water supply system, a photovoltaic power generation system, a wind power generation system, and introduction of clean energy fueled automobiles. Annual supply of 221,790 GJ was estimated. The major components are the automobiles, solar heat hot water system, and power generation systems. (NEDO)

  6. New energy vision of Oshimizu Town; 2001 nendo Oshimizu machi chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Oshimizu Town, Ishikawa Prefecture, an investigational study was conducted of the energy consumption amount of the city, existence amount of new energy and project for new energy introduction, and a vision was worked out. The energy consumption amount of the city was 3.02 x 10{sup 11} kcal, out of which electric power was 1.53 x 10{sup 11} kcal, petroleum 1.33 x 10{sup 11} kcal, and LPG 1.59 x 10{sup 10} kcal. As the project for new energy introduction, the following were studied: introduction of photovoltaic power generation to the general center/Oshimizu Junior High School/general household; solar energy utilization for the general center/general household; introduction of wind power generation to the Azuma Line of forest road; biomass power generation project using cattle feces/hog feces; RDF project for waste from Oshimizu Town; introduction of clean energy vehicle (hybrid car/electric car). The amount of new energy introduction from these was estimated at 1,716kL in crude oil conversion, which is equal to 5.3% of the energy demand amount. (NEDO)

  7. Report on energy saving vision in Santo-cho region; Santocho chiiki sho energy vision hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    An energy-saving vision was decided on in Santo-cho region in Hyogo Prefecture, with its outline reported. This town is such that about 80% of the region is mountains, forests and wilderness and that aging is advancing at the rate above that of Hyogo Prefecture or the national average. Nearly entire energy of the town is dependent on the supply from outside. The energy consumption is somewhat increasing as a whole, with that of the people's livelihood/domestic sector and of transportation sector are rising. In the classification of fuels, electricity is growing in consumption. As an energy-saving vision, it aimed principally at personal surroundings in which every one got into the habit of saving energy continuously without being forced. The basic plan for the energy conservation drive consisted of inducement to an energy-saving life style, energy conservation to be spread by the next generation children, continuation of energy saving activity rooted in the region, and promotion of energy conservation as a basis for introducing new energy. The diffusion and enlightenment for children destined to lead the next generation were defined as a particularly important assignment, as was the promotion of energy conservation and environmental education. (NEDO)

  8. New energy vision of the Kosaka town area; 2001 nendo Kosaka machi chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Kosaka Town, Akita Prefecture, an investigational study was conducted of the energy demand of the town, existence amount of new energy, project on new energy introduction, etc., and a new energy vision was worked out. The amount of energy demand of the town was 2,442,464 GJ/y. The energy demand consists of C heavy oil (about 1/3) and electric power (about 1/3) in the industrial sector, which are outstanding, and others including kerosene (about 12%) in the commercial/residential sector and light oil (about 10%) and gasoline (about 7%) in the transportation sector. The CO2 emissions from these are 60,000 t-C/y. As the project on the new energy introduction, study was made on the introduction of solar energy/snow cold energy space cooling to the insurance welfare center/interchange center, introduction of the photovoltaic power generation to the Korakukan area/elementary school/junior high school, introduction of the snow melting system using soil heat to town street/sidewalk, use of clean energy vehicle to public vehicle, use of ligneous biomass energy, etc. (NEDO)

  9. New energy vision in Kitakata City area; Kitakatashi chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A new energy vision was established in Kitakata City in Fukushima Prefecture. The population of Kitakata City in 2000 was 37,490, not having experienced extreme increase or decrease. Population employed in the tertiary industry occupies the largest ratio at 36.9%, followed by the secondary industry. The energy demand of the entire city is about 3300 trillion Joule, and carbon dioxide emitted therefrom is estimated about 77,000 t-C annually. Calculation of new energy amount in existence reveals about 4190 trillion Joule annually, which corresponds to about 1.3 times the energy demand quantity of the city. The new energy introducing project includes introduction of photovoltaic power generation into the public hall and schools, utilization of solar heat for greenhouses, introduction of solar heat and wind power hybrid power generation into the Green Tourism experiencing facilities, parks, street lights, and evacuation places, introduction of small to medium hydropower generation plants into Kitanogo, Kuranoyu and Happo-Ike, utilization of cold heat of snow, introduction of clean energy fueled automobiles into the public organizations, utilization of bio-mass energy, wastes burning power generation and heat utilization. (NEDO)

  10. Regional new energy vision for Sakurae town; Sakuraecho chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    The town is situated in a mountainous region typical of the Iwami district, Shimane Prefecture, rich in natural energy resources with as many as four hydroelectric power stations in service. Such local features were taken into account fully when a regional new energy vision was formulated for showing the town the course to follow for promoting the introduction of new energy which is environmentally friendly. The results of related efforts are described in eight chapters, which are (1) the outline of the vision, (2) survey of the trend of new energy, (3) description of Sakurae town, (4) townspeople's awareness of energy matters, (5) rate of energy consumption, (6) available amount of new energy, (7) basic policy for introducing new energy, and (8) projects for introducing new energy. Part (8) covers a photovoltaic project, wind power project, ligneous biomass energy introduction project, and the introduction of clean energy vehicles. Under the ligneous biomass energy introduction project, business profitability is discussed of a scrap wood fueled power plant. (NEDO)

  11. District energy-saving visions for Town of Towa; Towa machi chiiki sho energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    The energy-saving visions are drawn for Town of Towa, Iwate Pref., to promote energy saving and abatement of gas emissions which cause global warming. The priority projects in the energy-saving visions include introduction of energy-saving type vehicles, houses/buildings, illuminators; introduction of solar energy utilization systems and alternative energy (e.g., wood, wastes from domestic animals and microhydroelectric power generation); and monitoring/observation of energy consumption situations and energy-saving education. The energy-saving target is set at 3,120kL as crude oil. The energy-saving activities studied for the administrative and public sectors include introduction of energy-saving type vehicles and buildings; introduction of solar energy utilization systems (for roads and public facilities when they are replaced) and microhydroelectric power generation; and monitoring/observation of energy consumption situations and energy-saving education. The energy-saving target is set at 80kL as crude oil. (NEDO)

  12. New energy vision in Iwaizumi Township area; 2000 nendo Iwaizumicho chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A new energy vision was established in the Iwaizumi Township area in Iwate Prefecture. The heat energy consumed by each household in Iwaizumi Town is 20.5 two-hundred-liter drums, while transportation (railways, buses, trucks and private cars) consumes 12.2 drums annually, and the electric energy field 4.7 drums annually. As the town as a whole, the ratio of utilization as heat energy is relatively high. Therefore, new energy introduction should be focused importantly on use of solar heat hot water supply device and high-level utilization of forestry fuels. The new energy introduction project includes introduction of bio-mass energy to produce heat energy and electric power using livestock excreta, cut woods, thinned woods, and kitchen refuses as the resources, structuring a new energy model for the 'Communication Land Iwaizumi', activation of the central urban area by introducing micro hydropower generation, assistance to proliferation of new energies to town-operated houses, households, and business entities, introduction of new energies into the disaster prevention center, schools, and insurance, medical and welfare facilities, introduction of clean energy automobiles, and uplift of consciousness of town people. (NEDO)

  13. New energy vision of the Yokohama town area; 2001 nendo Yokohama machi chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Yokohama Town, Aomori Prefecture, an investigational study was conducted of the structure of energy demand of the town, existence amount of new energy, introduction of new energy, etc., and a new energy vision was worked out. The population of Yokohama Town was 5,508 as of October 2000, which is slightly decreasing. The number of household was unchanged, though. The amount of energy demand was estimated at 21,368 kL/y in crude oil conversion. The energy demand consisted of petroleum-base energy such as kerosene, light oil, heavy oil and gasoline (about 70%), electric power (20%) and LPG (10%). In the action plan for new energy introduction, the following were studied: wind power generation of which the plan has already been worked on by a private company (15 x 1,750kW class generator), introduction of the photovoltaic power generation to Yokohama Junior High School, hothouse vegetable cultivation using exhaust heat from hot spring, and 'Yokohama Rape Blossoms Eco Project' that mainly includes the agricultural land protection/oil squeezing from rapeseed/recycle of waste cooking oil as fuel, for which rape blossoms as one of the most important resources in Yokohama Town are used. (NEDO)

  14. Seismic microzonation on peak ground acceleration for Sapporo area; Sapporo chiiki no seismic microzonation

    Energy Technology Data Exchange (ETDEWEB)

    Hattori, S [Muroran Institute of Technology, Hokkaido (Japan); Vuetibau, L

    1997-10-22

    With an objective for use as fundamental information and data for measures to prevent disasters from regional earthquakes, microzonation was carried out on the Sapporo area. The present study has conducted comparisons and discussions on frequency distributions of maximum surface acceleration in the Sapporo area and the previously analyzed Muroran, Tomakomai and Obihiro areas. In the Muroran area, the frequency is distributed widely from 50 to 220 gal, while it is distributed narrowly from 110 to 170 gal in the Tomakomai area, and 90 to 160 gal in the Obihiro area. Such distributions have been formed possibly because the Muroran area has different kinds of grounds exist with hills and lowlands tangled, while the Tomakomai and Obihiro areas have nearly the same ground conditions with the topography being flat and the areas being located in an alluvial plain with soft Quaternary bed having been grown to a great thickness. The Sapporo area showed distribution close to that in the Muroran area. The maximum surface acceleration may vary even in the same mesh. This indicates that more detailed seismic microzonation is necessary to establish more effective means to prevent disasters from earthquakes. 10 refs., 4 figs., 1 tab.

  15. Regional new energy vision for Fukuoka city; Fukuokashi chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A regional new energy vision has been formulated for Fukuoka city, Fukuoka Prefecture, which will serve as a basic guide to the promotion of new energy introduction which is one of the measures for coping with environmental problems. The vision is described in five chapters of (1) the local characteristics of Fukuoka city, (2) energy situation in Fukuoka city, (3) basic program, (4) high priority plans, and (5) efforts to enhance the vision toward realization. Discussed as high priority plans are the introduction of new energy into public buildings above all others, high efficiency utilization of refuse-derived energy, utilization of new energy at water supply and drainage facilities, utilization of water temperature difference energy, introduction of clean energy vehicles, promotion of a new energy industry, and the extension of assistance for encouraging citizens and industrialists to adopt new energy. In extending assistance for encouraging the introduction of new energy, studies are made about measures for introducing new energy into individual residences and about a citizenry-involving new energy adopting industry (offering a site to the power generation industry) under the basal conditions that information be supplied sufficiently and that the citizenry and administration cooperate with each other in driving forward the vision. (NEDO)

  16. New energy vision of the Ehime prefecture area; 2001 nendo Ehime ken chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For the contribution to environmental preservation and stable supply of energy, 'a new energy vision of the Ehime prefecture area' was worked out which is to be a guide for new energy introduction using photovoltaic power, wind power, etc. In the Uma area, the following were proposed: introduction of waste energy cogeneration to the paper manufacturing industry; study of introduction of small wind turbine to household and business establishment. In the Niihama/Saijo area, introduction of cogeneration to business establishment; creation of the processing/assembly industry for materials/parts of equipment of photovoltaic power generation/wind power generation. In the Imabari area, introduction of natural gas cogeneration to business establishment, creation of the industry for equipment/parts of photovoltaic power generation. In the Matsuyama area, introduction of cogeneration to business establishment; utilization of biomass energy; creation of the industry of production/processing of resin for solar module use. In the Yawatahama/Oozu area, utilization of wind energy for agriculture/forestry, fisheries and tourism fields; study of introduction of biomass energy. In the Uwajima area, utilization of wind power energy for agriculture/fisheries, fisheries and tourism fields; introduction of biomass energy. (NEDO)

  17. New energy vision of the Noogata city area; 2001 nendo Noogata shi chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Noogata City, Fukuoka Prefecture, an investigational study was conducted of the energy demand amount of the city, existence amount of new energy, project for new energy introduction, etc., and a vision was worked out. The energy consumption amount of the city was estimated at 4,825.4 x 10{sup 6} MJ/y. It consisted of 47.1% in the industrial sector, 26.1% in the commercial/residential sector and 24.9% in the transportation sector. The rate of energy source was 65.7% of petroleum-base energy and 25.1% of electric power. As the project for new energy introduction, the following were studied: introduction of wind power generation/photovoltaic power generation to the flower park at the foot of Mt. Fukuchi and Nakanoshima park on the sandbank of the Onga river; introduction of photovoltaic power generation to library. Moreover, as future models of the introduction, the potential study was made on the following: installation of the stockbreeding waste biogas plant at the compost center; installation of fuel cell system using digestion gas from night soil treatment facilities; installation of the natural gas cogeneration system in the project on redevelopment of the urban area, etc. (NEDO)

  18. Regional new energy vision for Hita City; 2001 nendo Hita shi chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for elevating people's consciousness of such in Hita City, Oita Prefecture, surveys and studies were conducted concerning the energy supply/demand trend in the city, the amount of new energy resources in existence, and new energy introduction plans, and a vision was formulated. The city's energy consumption is estimated at 16,216,376 GJ, with the industrial sector responsible for 78.23%, the transportation sector for 11.35%, and the residential and commercial sector for 9.73%. It is in the public sector, general households, forestry, and hog raising that new energy will be utilized. Key projects for new energy introduction were discussed, which covered a residence incorporating a combination of new energies such as solar heat for water heating and photovoltaic power; lumber drying facilities utilizing forestry generated biomass; cogeneration fueled by methane gas from livestock excrements and garbage; photovoltaic power for schools, the city office, etc.; use of photovoltaic power, temperature difference energy, and methane gas at purification centers, etc.; use of clean energy vehicles for official use; etc. (NEDO)

  19. Magnetic structure of Akita-Yakeyama volcano; Akita Yakeyama chiiki no jikiteki kozo

    Energy Technology Data Exchange (ETDEWEB)

    Okuma, S [Geological Survey of Japan, Tsukuba (Japan)

    1996-10-01

    Akita-Yakeyama is a Quaternary volcano located in the north-western part of the Sengan geothermal area. Geothermal resource surveys have been performed from the middle of the 1970s in the Sengan geothermal area, and the Sumikawa geothermal power plant started its operation in 1995. By the analysis, the magnetic basement depth became lower in the north of Yakeyama body. However, the distribution including low magnetic anomaly in the south of Yakeyama body could not be explained only by a simple magnetic structure model. In this study, the magnetic anomaly in this area was analyzed in detail, and a construction of the magnetic structure model was intended by taking borehole data into account. The low magnetic intensity zone with low amplitude agreed well with the geothermal alteration zone. The high magnetic intensity zone and the low magnetic intensity zone corresponded with the distributions of volcanic rocks magnetized normally and reversely, respectively. Kakkonda and Matsukawa geothermal power plants were located in the distinct low magnetic intensity zones. There was a low magnetic intensity zone with long wave length over the whole Sengan geothermal area. A high magnetic intensity zone was observed in the northern foot of this area. From the magnetic measurements of borehole sample rocks, this was found to correspond with the newly formed granite body. 9 refs., 4 figs.

  20. Characteristics of microtremors in Hanshin area; Hanshin chiiki ni okeru bido no kihon tokusei nitsuite

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, H; Suzuki, K; Yamanaka, H; Seo, K [Tokyo Institute of Technology, Tokyo (Japan)

    1996-10-01

    To increase general understandings of fundamental characteristics of microtremors in Hanshin area, microtremors have been observed at Sumiyoshi River-side, Rokko Island, and Nishinomiya. Relations were discussed among the stability of predominant period, successive fluctuation and meteorological conditions. For the analysis, cosine-type taper was conducted before and after 10% of the observed records, and Fourier spectrum was calculated by smoothing using Parzen window with a band width 0.3 Hz. Geometrical mean of two components was used as a horizontal component. At the observation points except Sumiyoshi River-side, predominance in the short period side was not distinct, and was not stable due to the successive fluctuation of predominant period. However, there was less successive fluctuation of spectrum ratio between different two points in the band with period more than 1 sec. Since there was a close correlation between the successive fluctuation in this band and the wind velocity or air pressure, the microtremors in this band was affected by a single vibration source. Ground characteristics could be illustrated by the averages of ratio against base points of moving observation. 14 refs.

  1. Energy saving vision in Miyazu City region (FY2000); Miyazushi chiiki sho energy vision (2000 nendo)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-07-01

    An energy-saving vision was decided on in Miyazu City, Kyoto Prefecture, with its outline reported. The city promulgated the 'Miyazu City plan for the prevention of global warming' earlier this year, aiming at reduction in carbon dioxide exhaust by 25% in ten years ending 2010, and accordingly, also formulated the energy-saving vision on the basis of the plan. The measures with the citizens' life in mind were, for example, encouragement to participate in energy-saving monitor activity, publication of data of survey result, implementation of 'Miyazu eco-life school', support for citizens' voluntary action, utilization of public information/home pages, renewal of equipment and facilities, and preparation of performance/price data for new housing construction and so on. In addition, survey on actual condition, examination for improvements, etc., were among the measures intended for such sectors as hotels/inns, public transportation, manufacturing, and construction, and for the City administration itself. Furthermore, as measures to collaborate with energy suppliers, building an information exchange system is under consideration. (NEDO)

  2. Magnetotelluric (MT) surveying in the Kakkonda geothermal area; Kakkonda chinetsu chiiki deno MT ho tansa jikken

    Energy Technology Data Exchange (ETDEWEB)

    Ogawa, Y; Takakura, S [Geological Survey of Japan, Tsukuba (Japan); Uchida, T [New Energy and Industrial Technology Development Organization, Tokyo, (Japan)

    1997-05-27

    This paper describes the MT surveying conducted at the vicinity of a deep well (WD-1) in the Kakkonda geothermal area. For the MT surveying, the interval between measuring points was 300 m. Electric field dipole with a length of 300 m was given in the direction of traverse line, and that with a length of 50 m was given in the direction perpendicular to the line. Three components of magnetic field were measured using induction coil. Frequency band of the data was between 0.0005 Hz and 300 Hz. Characteristics of specific resistance model obtained from the MT surveying were illustrated. Low specific resistance zone less than 30 ohm-m was observed in the shallow zone below the altitude 0 m. This zone was comparable to the distribution of montmorillonite. High specific resistance anomaly was analyzed at the western half of the traverse line in the altitude between 0 m and -2000 m, which corresponded to the collective part of focuses. The bottom surface of the anomaly agreed well with the upper surface of neo-granite, i.e., the lower limits of earthquakes. Low specific resistance was observed in the altitude below -2000 m. 17 refs., 5 figs.

  3. New energy vision of the Akita city area; 2001 nendo Akita shi chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Akita City, an investigational study was conducted of the energy demand of the town, existence amount of new energy, project on new energy introduction, etc., and a new energy vision was worked out. The population of Akita City was 317,868 as of December 31, 2000, which is continuously on the increase. The amount of energy demand of the whole city was 990,000 kL/y. The energy demand consists of fossil fuels such as petroleum, coal and propane gas (77.7%) and electric power (22.3%). The CO2 emissions from these are 2,908,000 t-CO2. As the project on the new energy introduction, study was made on the installation of the photovoltaic power system and wind power system at base centers and introduction of the waste power system at the Goshono Works. As the model project, study was made on the introduction of the photovoltaic power generation and wind power generation to street light and public facilities, road snow melting/space heating and cooling using soil heat, introduction of clean energy vehicles, BDF introduction using waste cooking oil and rate blossoms, etc. (NEDO)

  4. Report on regional new energy vision for Kahoku town; Kahokucho chiiki shin energy vision hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    A regional new energy vision has been formulated for Kahoku town, Kochi Prefecture, which features the introduction of recoverable energy harmonious with nature and friendly to human life and environments. The results of activities conducted in this connection are compiled into nine chapters, which cover (1) Kahoku-town's local characteristics relative to new energy, (2) survey of townspeople's awareness of new energy, (3) amounts of new energy in existence, (4) basic policy toward introduction, (5) study of concrete measures for promoting introduction, (6) study of prioritized projects, (7) establishment of target amounts for introduction, (8) coordination with energy conservation measures, and (9) a system for thrusting forward the regional new energy vision toward embodiment. Chapter (7) comprises the way of setting target values for introduction; study of the target values to be achieved by the industry, administration, and citizenry, respectively; and a conclusion on the target values. Studies are conducted in the chapter about the introduction of solar energy, wind power generation, small scale hydroelectric power generation, cogeneration, clean energy vehicles, and the like. It is predicted that 960 kl/year will be introduced in fiscal 2010 in terms of oil, which accounts for 12% of energy consumption. (NEDO)

  5. New energy vision in Tochigi Prefectural area; Tochigiken chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    With an objective to structure a circulation type society that imposes less load on the environment, a new energy vision was established in the Tochigi Prefectural area. This plan is an overall plan to promote introduction of new energies, and at the same time serves as a guideline when prefectural residents, business entities and the administration attempt to introduce new energies. The plan was prepared upon viewing the year 2010. The new energy introduction quantity in 2010 was estimated to be 470,000 kL as converted into crude oil (4.7% of the consumption in fiscal 1997). The plan document covers the following five fields: 1) the basic conception of the vision establishment, 2) the current status and problems surrounding the energies, 3) the basic directionality in introducing new energies, 4) policy systems and role sharing toward introducing new energies, and 5) case studies and case presentation. Item 4 is composed of policy systems for new energy introduction, projects to be implemented with emphasis, roles of working organizations, and institutions for promotion. The projects to be executed with emphasis consist of five items including initiative introduction into facilities utilized by the prefectural residents. (NEDO)

  6. New energy visions for the Town of Ichinohe district; Ichinohecho chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    The new energy visions are drawn for Town of Ichinohe, Iwate Pref. The town's population has been generally decreasing since 1975, from 21,433 to 17,906 in 1995. The town consumed energy totaling 288,691Gcal in 1998, of which the transportation sector accounts for 55%, followed by the domestic, commercial and industrial sectors, in this order, the industrial sector accounting for only 7%. The estimated new energy sources available to the town are wind power, power generated by temperature difference at rivers or using snow, solar energy, biomass energy and energy from wastes, in the order of quantity, totaling 137GWh/y as electrical power and 45.6Tcal/y as heat. The priority projects include making Ichinohe Primary School an eco-school, construction of a biomass demonstration plant in the Okunakayama district, introduction of a boiler firing wood chips at Okunakayama Heights, construction of a wind power mill at Takamori Heights, and introduction of new energies by the citizens. (NEDO)

  7. Report on new energy vision in Fudai Village area; Fudaimura chiiki shin energy vision hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    A new energy vision was established in the Fudai Village area in Iwate Prefecture. The population of Fudai Village is decreasing from the peak of 4,103 persons in 1985. Its basic industry is fishery and agricultural industries, with the fish and marine product raising industry particularly active. The energy consumption in Fudai Village is, in the decreasing order, the transportation field (48.5%), the social and household field (26.5%), social industry, and manufacturing industry. Homes consume room heating energy at a greater extent. Energies are supplied by fuel oil at 53,554 Gcal (79.3%) and electric power at 11,551 Gcal (17.1%). The new energy introducing project has discussed introduction of cogeneration into the Kurosaki House, introduction of photovoltaic power generation and solar heat utilization systems, and introduction wind power generation as a tourism symbol. Discussions were also given on conversion of the automobiles used in the official organizations into clean energy automobiles. Further discussions were given on introduction of small new energy facilities, such as hybrid illumination, small wind power generation, and photovoltaic power generation, into the village office, the station square, the fish market, facilities in the fishing port, the children's hall, and the Road Station. (NEDO)

  8. Regional new energy vision for Yakumo village; Yakumomura chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A regional new energy vision has been formulated for Yakumo village, Shimane Prefecture, which abides with the village doctrines of introducing new energy for quality life and building a community advanced in terms of environmental protection. Yakumo village, which has a population of approximately 6,800 engaged mainly in agriculture or forestry, consumed 5 times 10{sup 5} GJ in energy in fiscal 1998. The results of activities conducted in this connection are summarized in nine chapters which are (1) the trends of affairs surrounding new energy, (2) Yakumo village's local characteristics as seen from the new energy viewpoint, (3) collection of information on new energy measures taken by local governments, (4) estimation of demand for energy in Yakumo village, (5) estimation of the existing amount and available amount of new energy in Yakumo village, (6) study of the basic concept and basic policy of the vision, (7) study of new energy introduction, (8) approach to the issue by thinking, and (9) reference materials. In concrete terms, discussion is held about introduction into public facilities of photovoltaic power generation and water heaters, wind power generation, cogeneration, and waste heat utilization. For the sake of gardening at public facilities, for instance, the introduction of photovoltaic power generation is taken up. (NEDO)

  9. Regional new energy vision for Aogaki town; Aogakicho chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A regional new energy vision has been formulated as part of Phase 4 of the Town Development Program of Aogaki town, Hyogo Prefecture, calling for 'building a town, comfortable to live in and full of hope, by utilizing rich natural environments.' The results of activities conducted in this connection are summarized in five chapters, which are (1) the description of the new energy vision, (2) Aogaki-town's energy characteristics, (3) basic plan, (4) driving forward of prioritized plans, and (5) toward the driving forward of the new energy vision. Chapter (4) comprises the goal of the formulation of the prioritized plans, the definition of the prioritized plans, and the establishment of individual projects. Established as the individual projects are the introduction of new energy linked to the inhibition of carbon dioxide gas emissions, use of wind energy for multiple purposes, public facilities preceding others in the introduction of new energy, building of environmentally symbiotic residences utilizing new energy, utilization of new energy at recreation facilities, and the popularization of and enlightening about new energy and its introduction through the participation of the local population. (NEDO)

  10. New energy vision in Mobara City area (Chiba Prefecture); Mobarashi chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    With the saying {sup M}obara, you find good when you live in it' as the target, the 'new energy vision for Mobara City area' was established. The vision is intended to utilize natural gas existing around the city, and utilize energy saving and new energy technologies. Specifically, the vision was established by a discussion committee composed of people of experience and academic standing, related business entities, and organizations, who took into consideration the natural environment, status of energy existence, and economic activities. The activity achievements were put into order by the following seven items: 1) the situation surrounding new energies, 2) district characteristics and viewpoints of introducing new energies, 3) energy demand, 4) existence quantity of new energies, 5) actual status of new energy introduction, and result of hearing investigations, 6) directionality and conception of introducing new energies, and 7) specific project objectives related to new energy introduction. Five projects were set up, including introduction of natural gas fueled automobiles, natural gas utilizing cogeneration and fuel cells, and effective utilization of wastes and bio-mass. (NEDO)

  11. New energy vision in Shioya Town area (Tochigi Prefecture); Shioyacho chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A new energy vision in Shioya Town area was established with objectives to make clear what is to be tackled by the administration and the residents in their respective standings, and elevate their consciousness to the environmental preservation and consciousness regarding the energy problems. The target for new energy introduction quantity in fiscal 2010 was set as CO2 emission quantity reduction to about 3,900 t-c/year, a reduction of 5% of the quantity in fiscal 1999. The activity achievements were put into order by the following seven items: 1) summary of implementing investigations, 2) basic district characteristics, 3) the current status and estimation of energy consumption, 4) estimated calculation on possibility of new energy utilization, 5) discussions and problems on possibilities of the introduction as seen from the district characteristics, 6) the new energy vision, and 7) the future assignments. Item 6 is composed of the introduction target, measures for introduction to achieve the target in fiscal 2010, and development of the leading projects. The projects include small to middle size hydropower generation, photovoltaic and wind power hybrid type power generation, and introduction of clean energy fueled automobiles. (NEDO)

  12. New energy vision in Murone Village area; 2000 nendo Muronemura chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A new energy vision was established in the Murone Village area in Iwate Prefecture. The population of the village as of 1995 was 6,552 persons, which has continued with slow decrease in the last decade, revealing remarkable departure from agriculture. Energy consumption in this village per person is 11.5 two-hundred-liter drums, and carbon dioxide discharge per person is 5.6t-CO2. This is about 60% of the national average, because of no existence of thermal power plants and factories. The existing quantity of new energies is occupied by bio-mass as the largest because more than 70% of the village area is forest, followed by solar beam and heat. However, in the case of forest, since the energy is actually utilized only from the felled woods, the utilizable quantity is considerably small, whereas the availability of solar beam and heat is higher. With regard to introduction of new energies, the efforts of the introduction thereof are directed to introduction into primary schools, the village office, the special nursing home for elderly people, and the meal providing center for middle schools, in addition to proliferation and enlightenment of household solar systems, and photovoltaic power generation systems. Furthermore, discussions were given on introduction of a pellet burning power plant utilizing waste woods. (NEDO)

  13. Regional new energy vision for Joetsu City; 2001 nendo Joetsu shi chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for enhancing people's consciousness of such at Joetsu City, Niigata Prefecture, surveys and studies were conducted involving energy consumption of the city, the amount of new energy resources in existence, and new energy introduction projects, and a vision was formulated. The city consumes 3,593 times 10{sup 9} kcal in energy, with the industrial sector responsible for 47.5%, the transportation sector 28.1%, and the residential/commercial sector 24.3%. As for the energy type, oil based fuel accounts for 58.3%, electric power 26.8%, and town gas 8.6%. The amount of carbon dioxide emissions due to energy consumption is estimated at 1,160 kt-CO2. Studies conducted for new energy introduction covered the supply of photovoltaic power to a project on municipal housing construction and a project on promoting the buildup of environment preservation facilities; the installation of a photovoltaic power generation system for a Citizens' Forest buildup project; the installation of a wind power generation system at the Minowadai Square of Recreation and Relaxation for the citizens; the installation of eco-stations to deal with gasoline, propane gas, and natural gas; the extension of financial support for the purchase of low-pollution vehicles; and so forth. (NEDO)

  14. Regional new energy vision for Tadami Town; 2001 nendo Tadami machi chiiki shin energy vision

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for enhancing people's consciousness of such at Tadami Town, Fukushima Prefecture, surveys and studies were conducted involving the energy demand characteristics of the town, the amount of new energy resources in existence, and new energy introduction projects, and a vision was formulated. Though there is not a great change through the year in town's demand for power, more power is necessary in winter for heating and lighting. As for demand for gas and oil, demand for heating oil reaches its peak in winter. New energy introduction projects were discussed, which would utilize snow for the dynamization of industries, and studies were conducted about the selection of items to be stored in snow rooms, selection of proper cold heat utilization systems, care to be taken in the designing of facilities, installation of snow rooms, development of specialty goods proper to the town, and so forth. Eco-school development was also studied as an environmental education project harmonizing with the local circumstances. Discussed for the fostering of welfare were an energy business for a Welfare House, snow handling measures linked to new energy utilization systems, and the like. (NEDO)

  15. Manufacturing process of rewritable CD and DVD; Kakikae gata CD, DVD no seizo process

    Energy Technology Data Exchange (ETDEWEB)

    Horie, M. [Mitsubishi Chemical Corp., Tokyo (Japan)

    1998-11-05

    Development of rewritable DVD is desired which has feedback compatibility with the existing players and ROM drives. The DVD is formed on a substrate with a thickness of 0.6 mm (dummy plate), and is composed of the multilayer film, i.e., protective coating made of UV hardening resin, reflective layer, dielectric protective layer, phase change type recording layer, and polycarbonate layer from the lower in order. A pair of dummy plates are pasted to enhance the mechanical strength, which has the same thickness of 1.2 mm as CD. Since the grooves and prepits are very fine, injection molding is more difficult compared with CD. Since a great stress change occurs during the formation and initialization processes of multilayer of phase change recording medium layer which is particular to DVD manufacturing process, control of warping is significant. Control of warping of the substrate is also significant, which affects the pasting and the whole process. The recording layer, dielectric protective layer and reflective layer are formed by the discharge spattering. The initialization is a particular process to the phase change medium. Complete non-recording state can be obtained by crystallizing the amorphous recording layer after the film formation. 3 figs.

  16. Static characteristics of a pilot relief valve; Baransupisuton gata ririfu ben no sei tokusei

    Energy Technology Data Exchange (ETDEWEB)

    Washio, S.; Yonguang YU; Nakamura, Y. [Okayama Univ. (Japan). School of Engineering

    1997-09-25

    Among a lot of hydraulic valves, relief valve is the most fundamental and important valve which takes change of pressure control. It is essential to know the working characteristics accurately, in order to predict the performance of . system. So far, the numerical simulation of relief valve has been tried and the mathematical models of individual component characteristics based on the knowledge of hydraulics are proposed. In this report, it was revealed that the static relation among the pressure drop, flow rate and opening area for a constriction can be represented, not by the traditional hydraulic orifice equation which has always been used for the purpose but by a new one including an additional pressure loss proportional to the flow rate and the fluid viscosity and inversely proportional to the square of the opening area. The new characteristic equation has proved to consistently forecast the experimental findings in which the rise in oil temperature results in an increase in the piston displacement, but causes little changes as regards regulated pressure. It has also turned out that contrary to conventional preconception, the fluid force exerted on a poppet is negligible. 6 refs., 14 figs., 1 tab.

  17. Estudo retrospectivo de 207 casos de tumores mamários em gatas

    Directory of Open Access Journals (Sweden)

    Monique Togni

    2013-03-01

    Full Text Available Este estudo teve como objetivos determinar os tumores mais prevalentes em gatos e relacionar os tumores mamários a alguns de seus fatores prognósticos. Os arquivos do Laboratório de Patologia Veterinária (LPV da Universidade Federal de Santa Maria (UFSM foram revisados e um total de 1.427 protocolos de biopsias e necropsias de felinos, entre 2000 e 2011, foi encontrado. Com base nas informações dos arquivos, foi estabelecida a relação entre os tumores e alguns fatores como sexo, idade, raça, estado reprodutivo, uso de contraceptivos, número e localização das glândulas afetadas, ulcerações, tamanho do neoplasma, metástases distantes e para os linfonodos. Assim, observou-se que os tumores de mama foram o segundo diagnóstico mais prevalente, após os tumores de pele. Todos os gatos com tumores mamários eram fêmeas, sendo os sem raça definida e os idosos os mais afetados. Os neoplasmas malignos foram diagnosticados com maior frequência, seguidos pelos tumores não neoplásicos e pelos neoplasmas benignos. Os tumores menores eram, na sua maioria, carcinomas. Ulcerações estavam presentes não só em neoplasmas malignos, mas também em alterações não neoplásicas. Metástases distantes foram encontradas principalmente nos pulmões e na pele.

  18. A single oncogenic enhancer rearrangement causes concomitant EVI1 and GATA2 deregulation in leukemia

    NARCIS (Netherlands)

    Gröschel, Stefan; Sanders, Mathijs A; Hoogenboezem, Remco; de Wit, Elzo; Bouwman, Britta A M; Erpelinck, Claudia; van der Velden, Vincent H J; Havermans, Marije; Avellino, Roberto; van Lom, Kirsten; Rombouts, Elwin J; van Duin, Mark; Döhner, Konstanze; Beverloo, H Berna; Bradner, James E; Döhner, Hartmut; Löwenberg, Bob; Valk, Peter J M; Bindels, Eric M J; de Laat, Wouter; Delwel, Ruud

    2014-01-01

    Chromosomal rearrangements without gene fusions have been implicated in leukemogenesis by causing deregulation of proto-oncogenes via relocation of cryptic regulatory DNA elements. AML with inv(3)/t(3;3) is associated with aberrant expression of the stem-cell regulator EVI1. Applying functional

  19. Molten carbonate fuel cell power generation system. Yoyu tansan prime en gata nenryo denchi hatsuden sochi

    Energy Technology Data Exchange (ETDEWEB)

    Uematsu, K; Hatori, S [Ishikawajima-Harima Heavy Industries Co. Ltd., Tokyo (Japan)

    1991-11-01

    In an indirect internal reforming type molten carbonate fuel cell, the reforming temperature is limited less than the operating temperature of the fuel cell, as the heat source for reforming is depended on the reaction heat at about 650 {degree} C of the fuel cell. To improve the reforming rate at the low temperature range, it is considered to increase S/C (ratio of steam to carbon), but this scheme will cause such problems as to increase the voltage drop of the anode, to drop the heat recovery ratio on the cogenerator, to increase the capacity of the heat exchanger, etc. In this invention, in the power generating plant of a molten carbonate fuel cell the inert gas is added to the reforming raw gas, and in addition to the above the gas is mixed with steam and led into the reforming chamber of the plant. When the inert gas which is not directly concerned in the reforming reaction is added to, total mol number increases and the reforming reaction is acceralated. Consequently, the reforming rate can be raised, though the reforming temperature is low. 2 figs.

  20. Direct regulation of Gata3 expression determines the T helper differentiation potential of Notch

    NARCIS (Netherlands)

    Amsen, Derk; Antov, Andrey; Jankovic, Dragana; Sher, Alan; Radtke, Freddy; Souabni, Abdallah; Busslinger, Meinrad; McCright, Brent; Gridley, Thomas; Flavell, Richard A.

    2007-01-01

    CD4(+) T helper cells differentiate into T helper 1 (Th1) or Th2 effector lineages, which orchestrate immunity to different types of microbes. Both Th1 and Th2 differentiation can be induced by Notch, but what dictates which of these programs is activated in response to Notch is not known. By using

  1. Mutational Analysis of GATA4 and NKX2.5 Genes in Dilated ...

    African Journals Online (AJOL)

    All rights reserved. Available ... Results: Evaluation of the age and sex of the patients indicated that DCM was more prevalent among ... NKX 2.5 are essential for cardiac development, and .... severe left ventricular dysfunction and a lower.

  2. Supplymentary type semiconductor device and manufacturing method. Soho gata handotai sochi oyobi sono seizo hoho

    Energy Technology Data Exchange (ETDEWEB)

    Uno, Masaaki

    1990-01-08

    As a supplementary type semiconductor device has a complicated structure, it is extremely difficult to construct it in a three dimensional structure. This invention aims to reduce its occupying area by forming p-channel and n-channel transistors in a solid structure; moreover in an easy method of production. In other words, an opening is made in the element-forming region of a semiconductor substrate, forming a gate-insulation film on each of the p-type and n-type semiconductors which are exposed on the two facing surfaces; on it formed a gate electrode; p-type semiconductor surface is used as a channel domain; a drain region of n-channel transistor on one surface and a source region on another surface; the n-type semiconductor surface corresponding to the gate electrode is used as a channel region; a source region of the n-channel transistor is formed on the same surface and the drain region on the substrate surface. Occupied area is thus made less and the production gets easier. 20 figs.

  3. Achievement report for fiscal 1999 on auxiliary New Sunshine Project. Development of coal hydrogasification technology (Survey and research on its social acceptability); 1999 nendo New Sunshine keikaku hojo jigyo seika hokokusho. Sekitan suiso tenka gaska gijutsu kaihatsu - Shakai tekigo sei ni kansuru chosa kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    With an objective to evaluate feasibility of practical use and economy of the coal hydrogasification technology (the ARCH process), survey and research have been performed. This paper summarizes the achievements in fiscal 1999. In the survey on the social acceptability, survey has been made on the future trend in the demand and supply and the price of LNG, LPG, and coal for hydrogasification. As a result, it was discovered that the price of LNG imported into Japan is determined as if linked with the crude oil price, and Saudi Arabia is the price leader of the LPG price. With respect to the survey on the possibility of international cooperation, surveys were conducted on the prospects of the long-term demand and supply in China, natural gas resources, and the demand and supply thereof. The feasibility study has estimated the product gas manufacturing cost after the process has been improved. In the trial calculation on the three-mode cost, it was discovered that, although the profit from byproducts is great, the BTX maximized mode causes the manufacturing cost to be higher by as much as 2 to 3 yen per Nm{sup 3} than that of other modes because of higher unit consumption in raw materials and higher construction cost. (NEDO)

  4. R and D of suitable unit to heightening in grade of mobile wireless telegraphy system; Test on data transmission characteristics and research on transmission method. Ido musen system no kodoka ni tekigo kano na sochi no kaihatsu kenkyu; Deta denso tokusei shiken oyobi denso hoho no kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Onigawara, Shuichi; Koyama, Masayoshi; Masunaga, Katsutoshi [Kyushu Electric Power Co., Inc., Fukuoka (Japan)

    1989-03-30

    In order to heighten the grade by the present mobile wireless telegraphy system, through the experimentation and research on both data transmission and error correction methods, both data and facsimile transmission were confirmed to be possible together with the following result: In the character transmission by a CPU connection and other encoded transmissions in which even an error by one bit considerably influences the contents of data, 1200bps, as able to be widened in service area, is profitable. While the data transmission in the mobile wireless telegraphy generates many errors due to phasing and noise along the propagation route. To attenuate those errors, error correction encoding is effectively applied with a correction method to be selected by kind of data. Further the 150MHz band, as more largely influenced by urban noise than the 400MHz band, narrows service area in the urban district. While the mobile wireless telegraphy is restricted in frequency band region. 12 refs., 26 figs., 9 tabs.

  5. FY 2000 report on the survey of extraction of areas promising in strategic geothermal development. Kurikoma peripheral region; 2000 nendo senryakuteki chinetsu kaihatsu yubo chiiki chushutsu chosa hokokusho. Kurikoma shuhen chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    An investigational study was conducted for the Kurikoma peripheral region with the aim of contributing to the selection of important areas for survey, plans to be worked out for introduction of medium-term/long-term and effective geothermal development promotion survey, estimation of the developmental resource amount in the areas, etc. In the study, based on the geological survey, geochemical survey, physical survey and well survey which were conducted directly for the Kurikoma peripheral region, the data needed to elucidate geothermal structure elements were extracted and re-analyzed to make geothermal models. Based on the geothermal system conceptual model made, the data on the areas already developed, etc. were arranged to make criteria for extraction of promising areas. By the criteria, the following three areas were selected, and geothermal structure models were made for the areas: promising area 1 in geothermal development (Sanzugawa-Oyu spa, 66km{sup 2}), promising area 2 (Wasabizawa-Akinomiya area, 37km{sup 2}), promising area 3 (Onikobe-Kamitaki spa, 21km{sup 2}). These promising areas were evaluated by the resource amount density, data likelihood, regulation by the natural park law, etc. Important areas for survey were selected and the resource amount was calculated. (NEDO)

  6. FY 2000 report on the survey of extraction of areas promising in strategic geothermal development. Hachimantai south region; 2000 nendo senryakuteki chinetsu kaihatsu yubo chiiki chushutsu chosa hokokusho. Hachimantai nanbu chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    An investigational study was conducted for the Hachimantai south region with the aim of contributing to plans to be worked out for introduction of medium-term/long-term and important geothermal development promotion surveys, estimation of the developmental resource amount, etc. In the survey, the data were widely collected/arranged on the geothermal development surveys so far made in the Hachimantai south region, and wide-area geothermal system conceptual models in this region were constructed. Based on the wide-area geothermal system conceptual models and the manual for strategic survey, the following were extracted as areas for which the existence of geothermal reservoir structures is expected: Toshichi spa and the southeast area, Kakkonda-Matsukawa-Iwateyama area, Nyuto spa and the southwest area. Further, from the criteria for extraction such as the resource amount density, the natural park law and data likelihood, extracted were the Moroedake area (9km{sup 2}), Matsukawa east area (10km{sup 2}), Omatsukurayama south area (9km{sup 2}) and Kakkonda east area (14km{sup 2}). In the Monte Carlo analysis by the stored heat method, the resource amount was estimated as 5MWe, 14MWe, 4MWe and 48MWe, respectively. (NEDO)

  7. Report on strategic survey on promising areas for geothermal development in fiscal 1999. Hohi Area (second report); 1999 nendo chinetsu kaihatsu yubo chiiki wo taisho to shita senryakuteki chosa hokokusho. 2. Hohi chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    This paper describes a strategic consideration on promising areas for geothermal development in fiscal 1999. Areas of importance to be given surveys corresponding to the geothermal development promotion survey range C were extracted from the promising areas extracted in the previous year, based on further objective analysis and evaluation criteria. Geothermal structure models were prepared on each promising area from geological and geothermal structural elements according to the standard method for the geothermal structure model structuring technology. Amount of geothermal resources in the promising areas was evaluated by using the evaluation supporting tools. In order to extract the areas of importance to be given the surveys, the extraction criteria were discussed based on the resource density, the Natural Park Law, and the data accuracy. Furthermore, comprehensive evaluation was given on geothermal structure properties, location and environmental conditions based on the geothermal structure models. Areas expected of effective achievements in the development promotion survey were selected, and a reservoir conception model was prepared. Assuming the single flash power generation, the resource amount was evaluated by using the Monte Carlo analysis of the Stored Heat Law. Social and environmental issues were also considered. A draft of the optimum survey program corresponding to each survey stage was prepared, with the areas of importance to be given the survey as the object. (NEDO)

  8. FY 2000 report on the survey for extraction of areas promising in strategic exploration geothermal development. Kirishima region; 2000 nendo senryakuteki chosa chinetsu kaihatsu yubo chiiki chushutsu chosa hokokusho. Kirishima chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    An investigational study was conducted for the Kirishima region with the aim of contributing to working out future medium- and long-term plans of geothermal development promotion survey, estimation of the developmental resource amount, extraction of technology development subjects effectively connecting to the increase in kW, etc. In the study, literature is collected such as reports of the surveys already made in the Kirishima region and the periphery, the data were re-analyzed and divided into geological structure elements and geological structure elements, and a conceptual model of the geothermal system in the whole Kirishima region was made. As a result, two areas were extracted for which the existence of high temperature geothermal reservoirs is expected. The total resource amount was estimated at 2,785MWe at promising area 1 and at 3,237MWe at promising area 2. With the resource amount density, data likelihood and distribution status of national/quasi-national parks as extraction criteria, 6 out of these promising areas were focused on important areas for survey. Geothermal structure models were made for each of the 6 areas, and the evaluation of the resource amount using evaluation assist tools was carried out. (NEDO)

  9. Report on new energy vision establishment in Oga City area; Ogashi chiiki shin energy vision sakutei hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A new energy vision was established in Oga City in Akita Prefecture. The population of the city is continuing the decreasing trend since 1955 to 30,462 persons as of 2000. The number of employed people by industry is in the trend of decreasing in the primary industry, leveling off in the second industry, and increasing in the tertiary industry. The annual energy demand in this city is 838,000 MWh in electric power and 302 times 10{sup 13} J, accounted for by the consumer department at about 44.4%, the transportation department at about 28.3% and the industrial department at 27.3%. The carbon dioxide emission amount therefrom is estimated 68,100 t-C. The expected usable quantity of new energies in the city would be available in solar heat as the largest source, followed by solar beam and wind power, whose amount is estimated 136.41 times 10{sup 13}J annually in total. The new energy introducing project includes introduction of wind power generation into Namahage Line and its vicinity and the Ikari area, manufacture of fuel from refuses, introduction of clean energy fueled automobiles into the cars used by the city office, introduction of photovoltaic into the general gymnasium and primary and middle schools, and solar heat utilization at the general gymnasium. (NEDO)

  10. MT2-D inversion analysis in Kakkonda geothermal field; Kakkonda chinetsu chiiki ni okeru MT ho nijigen kaiseki

    Energy Technology Data Exchange (ETDEWEB)

    Yamane, K; Takasugi, S [Geothermal Energy Research and Development Co. Ltd., Tokyo (Japan); Miyazaki, S [Japan Metals and Chemicals Co. Ltd., Tokyo (Japan); Uchida, T [New Energy and Industrial Technology Development Organization, Tokyo, (Japan)

    1996-05-01

    Data, collected from an MT method-assisted survey conducted in the Kakkonda geothermal region in 1987, was re-examined, and a new structure was found. The review was carried out by use of a 2D analysis in the TM mode. According to the 1D analysis of 1987 and the geological data gathered then, it is estimated that the resistivity structure of this region runs in the northwest-southeast direction. A northeast-southwest traverse line was set for this analysis, orthogonal to the strike, and the impedance at each observation spot was caused to rotate to this direction across the whole range of frequency. Furthermore, in 1994-95, surveys were conducted using arrayed CSMT/MT methods. All these sum up to indicate that a high-resistivity region extends northwest in the southwestern part of the Kakkonda river but that there exists a low-resistivity region of several 10 Ohm m centering about the B traverse line. The high-resistivity region deep in the ground being the target of excavation in the Kakkonda region, to collect knowledge about this high-resistivity is important, and here the effectiveness of the 2d analysis has been verified. 5 refs., 11 figs.

  11. Report of regional new energy vision for Kannami Town; 2001 nendo Kannami cho chiiki shin energy vision hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For promoting the introduction of new energy and for enhancing people's consciousness of such at Kannami Town, Shizuoka Prefecture, surveys and studies were conducted involving the energy consumption structure of the town, the amount of new energy resources in existence, and new energy introduction projects, and a vision was formulated. The town consumes approximately 5-million GJ/year, with the industrial sector consuming 47%, the transportation sector 29%, and the residential and commercial sector 26%. When a comparison is made, the percentages of the residential/commercial and transportation sectors are relatively higher than the national average. Projects for new energy introduction were studied, which involved photovoltaic power generation for the town office, community hall, primary and junior high schools, nursery schools, and general residences; solar heat utilization for public facilities, greenhouses, and general residences; wind power generation for sightseeing facilities, public gardens, and emergency evaluation areas; utilization of sewage waste heat for the sewage purification center; utilization of hot spa waste heat in the urban area; a biomass-fueled power plant; medium- and mini-scale hydroelectric power generation for the drinking water treatment plant; refuse-fueled power generation at refuse incineration facilities; use of clean-energy automobiles; natural gas-fueled cogeneration for public organizations; and so forth. (NEDO)

  12. New energy vision of the Gushikami Village area; 2001 nendo Gushikami son chiiki shin energy vision sakutei tou jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Gushikami Village, Okinawa Prefecture, an investigational study was conducted of the energy demand of the village, existence amount of new energy, plan for new energy introduction, etc., and a vision was worked out. The energy demand of Gushikami Village was estimated at 640.2 x 10{sup 6} MJ/y. By sector, it consisted of 37.1% in the transportation sector, 32.0% in the commercial/residential sector and 30.8% in the industrial sector. The rate of energy source was 73.7% of petroleum-base fuel and 26.3% of electric power. As the plan for new energy introduction, the following were studied: introduction of photovoltaic power generation to the village office/museum/elementary school/junior high school, introduction of solar heat utilization facilities to the meal providing center/gymnasium, introduction of wind power generation to the sports park, project of wind power generation on the sugar cane hill (600kW x 6 turbines), cogeneration by composting of stockbreeding waste and methane, introduction of clean energy car to official vehicle, introduction of natural gas vehicle, installation of experimental plant for conversion of sugar cane to methanol, etc. (NEDO)

  13. Report on new energy vision at Hinase Town area; 2001 nendo Hinase cho chiiki shin energy vision hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    With an objective of accelerating introduction of and uplifting consciousness on new energies at Hinase Town in Hyogo Prefecture, investigations and discussions were given on the energy demand, quantity of new energies available, and the new energy introduction projects for the town, thus a new energy vision was established. The estimated amount of energy consumption in Hinase Town is 242,814,320,000 kcal in fiscal 2000. The amount of carbon dioxide discharge associated therewith is 76.57 t-CO2/year. Regarding the energy consumption by departments, the industrial department consumes the most, followed by the transportation department, civil household department, and civil business department. The new energy introduction projects have discussed servicing of the bio-gas plant utilizing organic wastes and cogeneration, the new energy introduction projects for the Natural Energy Island at Kakui Island and the ocean ranch consisted of the use of solar heat water warmer, solar cell powered street lights, photovoltaic power generation, and electric cars for sightseeing visitors, the Eco-school promotion project for primary schools, introduction of energy saving devices into the town office, and the new energy proliferation and enlightenment project. (NEDO)

  14. Fiscal 1999 edition. Guidebook for introducing new energies in Kinki District; Kinki chiiki shin energy donyu guide book

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-09-01

    The captioned guidebook is composed of the following subjects: (1) the current status and problems of new energies (the energy status and the current status of new energies in Japan, and approaches to new energies in Japan); (2) the status of introduction and works in Kinki District (the outline of introduction and works in Kinki District on photovoltaic electric power generation, solar heat utilization, wind power generation, wastes power generation, clean energy fueled automobiles, cogeneration, fuel cells, unused energies, and other reproducible energies); (3) NEDO related supportive institutions (new energy introduction promotion projects by NEDO, and the outline of different supportive institutions); (4) new energy introduction flow (total flow leading to the introduction, and introduction flow by new energies); (5) the grand new energy prize in Kinki District (what is the grand new energy prize? and cases of prizes awarded in Kinki District); (6) new energy related supportive institutions (a list of the related institutions, and the outline of different supportive institutions); and (7) new energy consultation windows and contacts (consultation windows and contacts for general new energy aspects and the government related supportive institutions, and consultation windows by new energies). The grand new energy prize is awarded by New Energy Foundation as the 'institution for public recognition of new energy devices of the 21st century type'. (NEDO)

  15. Report on a regional new energy vision establishment in Yatsuka Township; Yatsukamachi chiiki shin energy vision sakutei jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    Surveys and discussions were given on establishing a new energy vision in Yatsuka Township in Shimane Prefecture. For the survey on available amount of natural energies (sunlight and wind), all-weather insolation required for solar energy power generation was analyzed by using the NEDO information, and wind was analyzed based on observations at Matsue Meteorological Observatory, Sakai-minato Observatory, and Nakaura Water Gate. In discussing the system, power generation amount in the Matsue area was estimated for the case when solar cells of single crystal silicon plus amorphous hybrid type HIP-H48B1 are used. For wind power generation, power generation amount was calculated from observation values derived at the Nakaura water gate when wind mills with diameters from 2 to 10 m are used. Furthermore, calculations were performed on power amount generated uniformly by using a hybrid system using solar energy power generation and wind power generation. As a result of the discussions, a plan was established to introduce the new energies by means of a solar and wind energy hybrid power generation system to the greenhouse 'Green Stellar' symbolizing the Japan's largest peony production, being capable of year-round peony cultivation. The system is planned to be introduced to the town office building and other public organizations sequentially according to annual programs. (NEDO)

  16. Survey report on establishing a regional new energy vision in Gohoku Village; Gohokuson chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    A regional new energy vision was established in Gohoku Village in Kochi Prefecture. Significance of this vision as a guideline for new energy introduction lies in preservation of natural environment, development promotion of the district, and building of a village strong against disasters. According to the discussion on energy availability in Gohoku Village, the expected usable amount will be about 5.13 million kWh/year by using solar energy power generation, about 2.25 billion kcal/year by utilizing solar heat, about 42 million kWh/year by utilizing wind energy, about 1.07 million kWh/year by using a small hydroelectric power plant, and about 31 million kcal/year by using wastes energy. The important new energy introduction projects are planned for introducing solar energy power generation, solar heat utilization, wind power generation, and small hydroelectric power generation in the area of 'Green Park Hodono' to make it a new energy model park (an ecology park), introducing solar energy power generation and solar heat utilization in the health and welfare center, day-service center, and special aged nursing home, and introducing clean energy fueled automobiles in official use. (NEDO)

  17. Geothermal development promotion survey. No. B-3 Kumaishi area; Chinethu kaihatsu sokushin chosa seika hokokusho. No. B-3. Kumaishi chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    This paper summarizes the result of a geothermal resource survey carried out from fiscal 1995 through fiscal 1997 in the Kumaishi Area located in the western center of Oshima Peninsula in the south western part of Hokkaido. The survey for this area was performed with an objective to search the spread of high temperature area continuing from the adjoining Yakumo area where the highest temperature of 233 degrees C has been confirmed in a structural test well (N63-YK-6), to identify high water permeating areas in great depth points underground, and to find possibility of existence of geothermal resources. The survey included geological and alteration zone survey, geochemical survey, gravitation exploration, electromagnetic exploration, drilling and temperature logging of five structural test wells, water injection test, temperature restoration test, core survey and hot water survey. Also performed were environmental effect survey, rock age measurement, alteration age measurement, and fluid inclusion test. As a result of the survey, it was identified that the verified geothermal hot water has been stored in granites with poor water permeability, whose low temperature has not formed convection systems spreading in wide areas despite having been heated, making each system exist in small scale independently. It was estimated that the possibility is low to expect geothermal power generation. (NEDO)

  18. Fiscal 1999 edition. Guidebook for introducing new energies in Kinki District; Kinki chiiki shin energy donyu guide book

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-09-01

    The captioned guidebook is composed of the following subjects: (1) the current status and problems of new energies (the energy status and the current status of new energies in Japan, and approaches to new energies in Japan); (2) the status of introduction and works in Kinki District (the outline of introduction and works in Kinki District on photovoltaic electric power generation, solar heat utilization, wind power generation, wastes power generation, clean energy fueled automobiles, cogeneration, fuel cells, unused energies, and other reproducible energies); (3) NEDO related supportive institutions (new energy introduction promotion projects by NEDO, and the outline of different supportive institutions); (4) new energy introduction flow (total flow leading to the introduction, and introduction flow by new energies); (5) the grand new energy prize in Kinki District (what is the grand new energy prize? and cases of prizes awarded in Kinki District); (6) new energy related supportive institutions (a list of the related institutions, and the outline of different supportive institutions); and (7) new energy consultation windows and contacts (consultation windows and contacts for general new energy aspects and the government related supportive institutions, and consultation windows by new energies). The grand new energy prize is awarded by New Energy Foundation as the 'institution for public recognition of new energy devices of the 21st century type'. (NEDO)

  19. Basic survey report on regional new energy vision for Ehime Prefecture; Ehimeken chiiki shin energy vision kiso chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    A new energy vision has been formulated to establish guidelines for popularizing and utilizing new energy which generates but a small amount of environmental impact. The results of surveys conducted in this connection are described in five chapters, which cover (1) the trend of new energy, (2) current situation of new energy in Ehime Prefecture, (3) calculation of the existing amount and available amount of new energy, (4) calculation of the introducible amount of new energy, and (5) the basic policy toward the introduction of new energy. Part (4) covers solar energy, wind energy, waste-derived energy, temperature difference energy, minor scale hydroelectric power generation, biomass energy, and like others. It states that a total of 540,000 kl/year will be available in terms of oil, equivalent to approximately 6% of Ehime Prefecture's 1997 energy consumption, and that it is expected that in fiscal 2010 photovoltaic/passive solar energy utilization, cogeneration, and wind power will yield 152,000, 314,000, and 27,000 kl/year respectively. (NEDO)

  20. Regional new energy vision formulation for Nichinan City. Fiscal 2001 report; 2001 nendo Nichinan shi chiiki shin energy vision sakuteisho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For promoting the introduction of new energy and for elevating people's consciousness of such in Nichinan City, Miyazaki Prefecture, surveys and studies were conducted concerning the energy demand of the city, the amount of new energy resources in existence, and new energy introduction plans, and then a vision was formulated. The city's energy demand is estimated to be approximately 4,028-million MJ/year, which includes 46% for the transportation sector, 30% for the people's livelihood-related business sector, 17% for the residential sector, and 7% for the industrial sector. The energy demand is covered 62% by oil-based fuels and 30% by electric power. Projects discussed for new energy introduction included the introduction of photovoltaic power into a lifelong learning center, a comprehensive center for health and welfare, and primary schools; introduction of solar hot water supply systems into school meal providing facilities; introduction of medium-size wind power systems (five systems); medium- or small-scale hydroelectric power generation on the Sakatani river; use of street light lighted by photovoltaic/wind hybrid power systems; introduction of clean energy vehicles; power generation utilizing livestock excrement; introduction of photovoltaic, hydroelectric, and methane gas power generation into a sewage treatment plant; and the use of RPF (refuse paper and plastic fuel) at the Nichinan plant of Oji Paper Co., Ltd. (NEDO)

  1. FY 2001 Report of regional new energy vision for Futaba Town; 2001 nendo Futaba machi chiiki shin energy vision hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for enhancing people's consciousness of such at Futaba Town, Fukushima Prefecture, surveys and studies were conducted involving the amount of energy consumed by the town, the amount of new energy resources in existence, and new energy introduction projects, and a vision was formulated. The annual energy consumption of the town is 113,049 times 10{sup 6} kcal, with automobiles consuming 47.2%, manufacturing industries 19.8%, households 18.9%, and commerce 12.6%. The amount of carbon dioxide due to the consumption is estimated at 9,999 times 10{sup 3} kg-C in total. Studies were made about new energy introduction projects toward 2010, which involved the introduction of photovoltaic power generation systems and solar heat utilization systems into four public facilities and 214 general residences; introduction of nine clean energy vehicles into public facilities and 181 into general residences; a wind power station near the seashore; a mini-scale hydroelectric power system for irrigation; introduction of new energy for illuminating sightseeing roads and parks, and so forth. (NEDO)

  2. Report on establishing new energy vision at Furen Town area; 2001 nendo Furen cho chiiki shin energy vision sakutei hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    With an object of accelerating introduction of and uplifting consciousness on new energies at the city of Furen in Hokkaido, investigations and discussions were given on the energy demand, quantity of new energies in existence, and new energy introduction projects of the city, thus a new energy vision was established. The estimated amount of energy consumed in Furen Town is 119,846 x 10{sup 3} kWh. By departments, the transportation department has the highest consumption ratio, followed by general household. Energy types are kerosene, followed by heavy oil, electric power and LPG in the decreasing order. The important new energy introduction projects have discussed the ''Snow Storehouse Workshop'' being a low temperature rice storage facility in Bibai City, which performs cold storage and shipment adjustment of agricultural products, through-the-year agriculture to perform house cultivation by using rice straws and rice husks as the heat energy source, adjustment of temperatures of paddy fields by using calorific power of rice husks, electric power generation and heat supply by using heat of combustion of chips from lumber factories, electric power generation and heat supply by using livestock excreta (bio-gas), and introduction of new energies into schools and parks. (NEDO)

  3. Investigation report on establishment of new energy vision in Toyohashi area; Toyohashi chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    The 'new energy vision for Toyohashi (Aichi Prefecture) area' was established from the viewpoint that proliferation of new energy use, which is less familiar and higher in cost, requires induction by urban and environmental measures, and consistency with district characteristics. The achievements were put into order as the report on the Toyohashi area new energy vision and the investigation on the vision establishment. The vision is composed by the following four items: 1) basic matters concerning the vision, 2) ideas and policies of the vision, 3) major measures for introducing new energies, and 4) promotion of the vision. Item 3) presents the introduction measures, introduction project, and measure development by areas. Considerations on the introduction measures include enlightenment for proliferation, expansion in information provision, development of preferential treatments, initiative introduction into public facilities, and the measures to strengthen cooperative works by citizens, business entities, and the administration. The following four projects were set: introduction of wastes power generation into the Resource Production Center (wastes treatment plant), improvement of the surplus heat utilization facilities, and introduction of wind power generation into the General Agricultural Park. (NEDO)

  4. Investigation report on establishment of new energy vision in Toyohashi area; Toyohashi chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    The 'new energy vision for Toyohashi (Aichi Prefecture) area' was established from the viewpoint that proliferation of new energy use, which is less familiar and higher in cost, requires induction by urban and environmental measures, and consistency with district characteristics. The achievements were put into order as the report on the Toyohashi area new energy vision and the investigation on the vision establishment. The vision is composed by the following four items: 1) basic matters concerning the vision, 2) ideas and policies of the vision, 3) major measures for introducing new energies, and 4) promotion of the vision. Item 3) presents the introduction measures, introduction project, and measure development by areas. Considerations on the introduction measures include enlightenment for proliferation, expansion in information provision, development of preferential treatments, initiative introduction into public facilities, and the measures to strengthen cooperative works by citizens, business entities, and the administration. The following four projects were set: introduction of wastes power generation into the Resource Production Center (wastes treatment plant), improvement of the surplus heat utilization facilities, and introduction of wind power generation into the General Agricultural Park. (NEDO)

  5. Fiscal 2000 report on regional new energy vision for Tanoura town; 2000 nendo Tauracho chiiki shin energy vision hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    A new energy vision has been formulated to make an environmentally friendly community of Tanoura town, Kumamoto Prefecture, which is a 6,000-people town facing Shiranui Bay and consisting of seashore, the streets, and a mountainous district. The amount of new energy in existence, when converted into electric power, will be 10 GWh/year accounting for approximately 20% of 1999 power consumption. The types of new energy subjected to study are solar energy, wind energy, biomass energy, refuse-derived energy, and geothermal energy, with solar energy surpassing the rest. In the new energy vision, the roles of the administration, citizens, and industrialists are made obvious; new energy introduction projects are established for the introduction of photovoltaic power, solar heat, and wind power into Hot Spring Center; and clean energy vehicles will be acquired to serve as official vehicles. The new energy vision booklet comprises six chapters, with chapter 1 dealing with the basic philosophy of vision formulation. (NEDO)

  6. Georesistivity structure in the central part of North-Eastern Japan Arc; Tohoku chiho chubu chiiki no denki dendodo kozo

    Energy Technology Data Exchange (ETDEWEB)

    Fujinawa, Y. [National Research Institute for Disaster Prevention, Tsukuba (Japan); Kawakami, N. [GERD Geothermal Energy Research and Development Co. Ltd., Tokyo (Japan); Ueshima, M. [The University of Tokyo, Tokyo (Japan). Earthquake Research Institute; Honkura, Y. [Tokyo Institute of Technology, Tokyo (Japan)

    1998-03-13

    MT measurement of transects was made in the central part of North-Eastern Japan Arc to clarify tectonics of subduction zones. 1-D, 2-D and 3-D resistivity structures are observed in surface layers, and zones shallower and deeper than a Conrad surface, respectively. A main structure direction is S-N or NNE-SSW. Ishinomaki-Chokai tectonic line and low- resistivity zones due to Quaternary volcanos (Naruko, Onikobe) exist in a backbone range region. Resistivity is 100{Omega}{center_dot}m or less by Bostick Inversion except Mesozoic and Palaeozoic layers in the southern Kitakami mountainous region, resulting in a good agreement with previous results in a north transect. Resistivity is several {Omega}{center_dot}m and depth is several km around Shinjo basin and in surface layers of Kitakami River region. The backbone range region shows complex resistivity structures because of volcanic activity and wide-area hot water activity. High-resistivity layers correspond to stable Mesozoic and Palaeozoic land layers. Seismic velocity increases in the low-resistivity zone. Earthquake generally occurs at the boundary between resistivity structures. 68 refs., 9 figs., 2 tabs.

  7. Plan to promote new energy introduction in Niigata Prefecture area; Niigataken chiiki shin energy donyu suishin keikaku

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The 'Plan to promote new energy introduction in Niigata Prefecture area' was established to leave the conditions that everybody can live with affluence and comfort in the next generation. The plan lasts for ten years until 2010. Upon identifying the district characteristics of Niigata Prefecture, and based on the results of investigations on new energy existence quantity, utilization possibility thereof, and consciousness of residents of the prefecture, considerations were given that the plan shows the basic policy to promote proliferation of the new energies, and serves as the guideline for practical implementation. The plan document is composed of the following four items: 1) the foreword, 2) the current status of energies, 3) new energies expected of introduction, and 4) basic measures. The energy consumption was estimated to increase to 1.345 times that of fiscal 1990 in the year 2010, the increase being mainly in consumer and household use. The targeted quantity for new energy introduction was set to 90,000 kl annually as converted to petroleum. Expected new energy applications would include photovoltaic power generation, snow energy and solar heat utilization, and cogeneration utilizing natural gas. (NEDO)

  8. Report of the results of the fiscal 1997 regional consortium R and D project. Regional consortium energy field / research and development of a reverse engineering system for local industrial articles (first fiscal year); 1997 nendo chiiki consortium kenkyu kaihatsu jigyo. Chiiki consortium bun`ya / chiiki kogeihin muke reverse engineering system no kenkyu kaihatsu (daiichi nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    The paper described the fiscal 1997 result of the research on the application of local industrial article use reverse engineering (RE, it had been regarded as imitation and reproduction technology, but has recently been as a part of the production information integration). With the actual local industrial article as standard, which has features in design and was added with local traditionality, such as glass having 3D free-form surface, 3D shape information modeling technology RE is developed, and a system for direct automatic metal mold processing is established, aiming at shortening of delivery date, cost reduction and higher grade. The target of the research is Okinawa prefecture. In fiscal 1997, study was made of the speeding-up of the non-contact 3D shape information inputting (multi-point simultaneous inputting by CCD of the laser reflected light of the actual model) and the data storage technology. Cast iron was selected as molding materials, judging from reactivity/machinability with heat resistant glass. Also studied was the blend of glass materials suitable for press forming. Further, studies were proceeded with of development of intellectual cutting tools into which sensor function is integrated and of virtual reality for facilitating/accelerating the design up to metal mold manufacturing. 39 refs., 88 figs., 21 tabs.

  9. FY 2000 report on the results of the regional consortium R and D project - Regional new technology creation R and D. First year report. R and D of medicine toxicity assessment use hepatocyte chip; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo. Chiiki consortium kenkyu kaihatsu / chiiki shingijutsu soshutsu kenkyu kaihatsu. Dokubutsu dokusei hyokayo kansaibo chip no kenkyu kaihatsu (dai 1 nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Judging that in the beginning of the toxicity test, the necessary information/knowledge are acquired not by using animal itself but only by using liver system (hepatocyte), the development was proceeded with of the hepatocyte chip substituting for the animal experiment. Studies were made in the following five fields: 1) design of functional matrices; 2) development of the pharmacological assessment technology; 3) development of cell sensing technology/biosensor fabrication; 4) study of a possibility and the limit of cell patterning technology; 5) survey of the market scale. In 1), studies were made of the establishment of the 3D culture method for hepatocyte and the construction of hepatocyte models for medicine assessment using pieces of liver. In the latter, by including capsule using the sugar introduced with galactose residue which is special sugar of hepatocyte to alginic acid and chitosan which are natural polymer and also hepatocyte isolated into sponge, aggregate was formed, and its use as a medicine assessment use liver model was tried to be made. (NEDO)

  10. Preliminary study of airborne electromagnetic survey using grounded source; Chihyo source gata kuchu denji tansa no kisoteki kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Mogi, T [Kyushu University, Fukuoka (Japan). Faculty of Engineering; Shimoizumi, M [Kitakyushu Polytechnic College, Kitakyushu (Japan); Kusunoki, K [Central Research Institute of Electric Power Industry, Tokyo (Japan); Morikawa, T [Dowa Engineering Co. Ltd., Okayama (Japan); Jomori, N [Chiba Electronics Research Institute, Chiba (Japan)

    1996-05-01

    For the development of an airborne electromagnetic prospecting method capable of deeper exploration, a basic study was made about a system wherein a transmitter (source) is positioned on the ground and the receiving is done in the sky. Even in case of this airborne electromagnetic method, the TDEM method is supposedly advantageous over others as in case of groundborne exploration. In the study, the transient response of an airborne vertical magnetic field to a horizontal layered structure was calculated. The current source was 2000m long with a capacity of 30A. The one-layer structure was a 10 Ohm m semi-infinite ground, and the two-layer structure had a 100 Ohm m structure just under the one-layer structure. The result of the calculation suggests that, in the absence of a layer of extremely low resistivity, observation of an approximately 1 second long transient response aboard a helicopter flying at approximately 50km/h will enable an approximately 1000m deep exploration. Problems to affect airborne observation, such as swinging, natural magnetic field fluctuation, and artificially produced noises were investigated by use of a magnetometer suspended from a helicopter in flight. 2 refs., 6 figs.

  11. Studies on energy system for an energy-saving society; Sho energy gata shakai ni okeru energy system kento

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    The system to which new energy technology and energy saving technology were introduced was constructed for case studies of urban areas including core cities and the peripheral areas, and the quantitative analysis was conducted on environmental effects, etc. In the energy supply system model, the following element technologies were all considered: cogeneration system, sewage water heat, river water heat, the photovoltaic power generation, energy storage/heat storage/cold heat storage, adsorption type refrigerator, etc. Also considered were power interchange between clusters, system power buying/power selling, heat interchange or no heat interchange, etc. As a result, it was found that when constructing the energy system which synthetically takes into account thermoelectric ratios, rates of simultaneous loads, ratios of daytime/nighttime in the energy supply and demand in the urban area, the energy saving effect multiplicatively increases, and the energy system using cogeneration and unused energy such as refuse and sewage in the urban area and river water brings an energy saving effect of 32% especially in the concentrated cluster. 83 figs., 45 tabs.

  12. Drilling series. 4. ; Planning geothermal drilling (rotary type). Kussaku series. 4. ; Chinetsusei no kussaku keikaku (shutoshite rotary gata)

    Energy Technology Data Exchange (ETDEWEB)

    Ito, T. (S.K. Engineering Co. Ltd., Tokyo (Japan))

    1994-01-31

    The present report explained how to plan the drilling of geothermal well, and select the easing, drilling mud water and drilling rig in order to obtain the steam and hot water. The geothermal wells can be generally classified into exploration wells, production wells and reduction wells. The exploration well is a well to survey the underground strata, geological structure, and existence of steam and hot water, while the production well is a well to produce the steam and hot water. The reduction well is a well to condense the hot water produced by the production well and steam having passed through the power-generating turbine, and return them as condensate underground. The geothermal well is characterized by its high temperature, mud leakage, corrosive matter and scale, all of which make its drilling difficult and its management troublesome for the production and reduction. To plan the drilling, the order of processing are distinct conditioning of drilling differently by type of well, collection of geological survey data, programing for the casing and selection of drilling rig. The present report also gave the stress to affect the casing and standard of steel pipes to be used for the casing. 3 figs., 4 tabs.

  13. UTERINE PROLAPSE IN QUEEN AND UTERINE PROLAPSO UTERINO EM GATA E RETROFLEXÃO UTERINA EM CADELA

    OpenAIRE

    Giuliano Queiroz Mostachio; Wilter Ricardo Russiano Vicente; Diogo José Cardilli; Tathiana Ferguson Motheo; Gilson Hélio Toniollo

    2008-01-01

    Obstetrical emergencies are problem in veterinary clinics and hospital. So, the aim of this report is to describe the clinical-surgical aspect of one of them, the uterine prolapse. Complete protrusion and retroflexion of uterus had been diagnosed in a queen and female dog, respectively. After the stabilization of the animals and reduction of the prolapses followed by ovary-hysterectomy, one of the animals came to death due to septicemia and hypovolemic sho...

  14. IL-33 promotes GATA-3 polarization of gut-derived T cells in experimental and ulcerative colitis

    DEFF Research Database (Denmark)

    Seidelin, Jakob Benedict; Coskun, Mehmet; Kvist, Peter Helding

    2015-01-01

    of the immune response in experimental colitis (piroxicam-accelerated colitis (PAC) in IL-10 -/- mice, dextran sodium sulfate (DSS) model) and UC.METHODS: Colonic IL-33 expression was determined in UC (8 active UC, 8 quiescent UC, and 7 controls) and experimental colitis. Mesenteric lymph node (MesLN) T cells...

  15. Experimental study on power augmentation of Savonius rotor; Savonius gata fusha no shutsuryoku zokyo ni kansuru jikkenteki kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Sato, S; Kikuchi, K; Ushiyama, I [Ashikaga Institute of Technology, Tochigi (Japan)

    1997-11-25

    Wind power now being used is mostly for power generation, and the power generating rotor is represented by the horizontal propeller type. The vertical type, such as Savonius rotor which uses drag force, may be used for special purposes. The Savonius rotor has been used for water pumping-up and ventilation for its characteristics of low rotational speed and high torque. The authors have proposed, based on the data collected by operating a wind mill of 10W, a method for reducing resistance by deflecting wind flowing onto the return bucket to augment drag force, in an attempt to make the system more functional. The Savonius rotor is equipped with a semi-cylindrical cover, and guide and side plates, to follow their effects. It is found that these plates work to augment power without needing expansion of sweeping area. 4 refs., 12 figs.

  16. Fundamental study on airborne electromagnetic survey using grounded source; Chihyo source gata kuchu denji tansa no kisoteki kenkyu. 2

    Energy Technology Data Exchange (ETDEWEB)

    Mogi, T; Fujimitsu, Y [Kyushu University, Fukuoka (Japan). Faculty of Engineering; Tanaka, Y [Kyoto University, Kyoto (Japan). Faculty of Science; Jomori, N [Chiba Electronics Research Institute, Chiba (Japan); Morikawa, T [Dowa Engineering Co. Ltd., Okayama (Japan); Kusunoki, K [Central Research Institute of Electric Power Industry, Tokyo (Japan)

    1997-05-27

    With an objective to develop an airborne electromagnetic survey method for greater depths achievable of exploration, a discussion was given on an exploration method of a type in which a transmitting device is placed on the ground to receive signals in an atmosphere. A prototype exploration apparatus is mounted with a fluxgate magnetometer, an attitude meter, a GPS, and a battery. This exploration apparatus is suspended on a 30 meter long rope from a helicopter to perform the exploration. Two flight tests on this apparatus were carried out in the Unzen area, Nagasaki Prefecture and the Motomiya area, Wakayama Prefecture. The ground source was extended to a distance of 1.5 km, and a current of about 20 A was flown with a quiescent wave having four-second cycles. The helicopter flew nearly horizontally at a ground speed of about 50 km, a flight altitude of 450 m above sea level, and a terrain clearances of 100 to 400 m. The obtained data had variations in correspondence with changes in roll and pitch angles, whereas the variation of about 5000 nT was reduced to about 1000 nT as a result of correction. It was not possible, however, to correct completely the variation with short cycles, requiring further discussions on frequency characteristics of the magnetometer. 6 figs., 1 tab.

  17. FY1995 study of feedback type gait training system; 1995 nendo feedback gata hoko kuren sochi ni kansuru kenkyu kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    The purpose of this project is to develop and demonstrate the utility of feedback type gait training equipment designed for the measurement and evaluation by a walking training of the aged or patient. As similar concepts of walking training, a locomotion in the water for the aged is applied in rehabilitation. Our development of this study established the system of a suspending mechanism which revolves around the prop, and a walking on the circular type force plate by the aged or patient. It is possible to detect a walking reaction force of several patients from force plate simultaneously. And then, the data from force plate makes feedback signal to put up the patient like a buoyancy in the water. Concerning the evaluations of walking pattern a step range, a hanging ratio and a walking speed, etc. are acquired for each patient by the acknowledgment base. This system is actively able to perform a walking training continuously compared with conventional passive gait equipment. (NEDO)

  18. Studies of a thermal energy storage unit with ice on coils; Ice on coil gata kori chikunetsuso no kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Ito, S; Miura, N [Kanagawa Institute of Technology, Kanagawa (Japan)

    1996-10-27

    Study was made of an ice-on-coil heat storage tank for power load levelling. Prior to the prediction of performance of the system as a whole, the performance of the heat storage tank itself needs to be predicted. A brine (35.9% water solution of ethylene glycol) cooled by a refrigerating machine was poured from the upper end of the piping in the heat storage tank (consisting of 19 spiral pipes or coils arranged in parallel in the vertical direction) for the collection of ice around the coils. Ice grew thicker with the passage of time but there was no remarkable decrease in the transfer of heat because there was an increase in the area of contact between ice and water, and the brine exit temperature remained constant over a prolonged period of time. There was a close agreement between experiment results and theoretical conclusions throughout the heat accumulation process, including changes with time in the thickness of ice on the coils, all pointing to the appropriateness of this analytical effort. To melt the ice, water was poured into the tank top at a predetermined rate. Water chilly at 2-4{degree}C was recovered at the tank bottom, stable in the amount produced. As for the use of spiral pipes for making ice, the laminar heat transfer rate in such pipes are supposed to be more than two times higher than that in straight pipes, and this was quite effective in accelerating heat transfer. 7 refs., 11 figs.

  19. Quantitative controls on location and architecture of carbonate depositional sequences: upper miocene, cabo de gata region, se Spain

    Science.gov (United States)

    Franseen, E.K.; Goldstein, R.H.; Farr, M.R.

    1998-01-01

    Sequence stratigraphy, pinning-point relative sea-level curves, and magnetostratigraphy provide the quantitative data necessary to understand how rates of sea-level change and different substrate paleoslopes are dominant controls on accumulation rate, carbonate depositional sequence location, and internal architecture. Five third-order (1-10 my) and fourth-order (0.1-1.0 my) upper Miocene carbonate depositional sequences (DS1A, DS1B, DS2, DS3, TCC) formed with superimposed higher-frequency sea-level cycles in an archipelago setting in SE Spain. Overall, our study indicates when areas of high substrate slope (> 15??) are in shallow water, independent of climate, the location and internal architecture of carbonate deposits are not directly linked to sea-level position but, instead, are controlled by location of gently sloping substrates and processes of bypass. In contrast, if carbonate sediments are generated where substrates of low slope ( 15.6 cm/ky to ??? 2 cm/ky and overall relative sea level rose at rates of 17-21.4 cm/ky. Higher frequency sea-level rates were about 111 to more than 260 cm/ky, producing onlapping, fining- (deepening-) upward cycles. Decreasing accumulation rates resulted from decreasing surface area for shallow-water sediment production, drowning of shallow-water substrates, and complex sediment dispersal related to the archipelago setting. Typical systems tract and parasequence development should not be expected in "bypass ramp" settings; facies of onlapping strata do not track base level and are likely to be significantly different compared to onlapping strata associated with coastal onlap. Basal and upper DS2 reef megabreccias (indicating the transition from cool to warmer climatic conditions) were eroded from steep upslope positions and redeposited downslope onto areas of gentle substrate during rapid sea-level falls (> 22.7 cm/ky) of short duration. Such rapid sea-level falls and presence of steep slopes are not conducive to formation of forced regressive systems tracts composed of downstepping reef clinoforms. The DS3 reefal platform formed where shallow water coincided with gently sloping substrates created by earlier deposition. Slow progradation (0.39-1.45 km/my) is best explained by the lack of an extensive bank top, progressively falling sea level, and low productivity resulting from siliciclastic debris and excess nutrients shed from nearby volcanic islands. Although DS3 strata were deposited during a third-order relative sea-level cycle, a typical transgresse??e systems tract is not recognizable, indicating that the initial relative rise in sea level was too rapid (??? 19 cm/ky). Downstepping reefs, forming a forced regressive systems tract, were deposited during the relative sea-level fall at the end of DS3, indicating that relatively slow rates of fall (10 cm/ky or less) over favorable paleoslope conditions are conducive to generation of forced regressive systems tracts consisting of downstepping reef clinoforms. The TCC sequence consists of four shallow -water sedimentary cycles that were deposited during a 400 ky to 100 ky time span. Such shallow-water cycles, typical of many platforms, form only where shallow water intersects gently sloping substrates. The relative thicknesses of cycles (< 2 m to 15 m thick), magnitudes of relative sea-level fluctuations associated with each cycle (25-30 m), high rates of relative sea-level fluctuations (minimum of 25-120 cm/ky), and the widespread distribution of similar TCC cycles in the Mediterranean and elsewhere are supportive of a glacio-eustati

  20. Solar cell array for driving MOS type FET gate. MOS gata EFT gate kudoyo taiyo denchi array

    Energy Technology Data Exchange (ETDEWEB)

    Murakami, S; Yoshida, K; Yoshiki, T; Yamaguchi, Y; Nakayama, T; Owada, Y

    1990-03-12

    There has been a semiconductor relay utilizing MOS type FET (field effect transistor). Concerning the solar cells used for a semiconductor relay, it is required to separate the cells by forming insulating oxide films first and to form semiconductor layers by using many mask patterns, since a crystal semiconductor is used. Thereby its manufacturing process becomes complicated and laminification as well as thin film formation are difficult, In view of the above, this invention proposes a solar cell array for driving a MOS type FET gate consisting of amorphous silicon semiconductor cells, which are used for a semiconductor relay with solar cells generating electromotive power by the light of a light emitting diode and a MOS type FET that the power output of the above solar cells is supplied to its gate, and which are connected in series with many steps. 9 figs.

  1. Study on fault diagnostic system using modularized knowledge; Mojuru gata chishiki wo mochiita ijo shindan system ni kansuru kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Shimada, Y.; Sayama, H.; Suzuki, K. [Okayama Univ. (Japan). Dept. of Industrial and Mechanical Engineering

    1997-08-15

    Recently, a fault diagnostic expert system was prosperously developed as an objective of chemical plants and nuclear power plants. In this paper, a fault diagnostic method using modularized knowledge was proposed, a fault diagnostic system was constructed for an experimental plant, and the effectiveness of this method was clarified by carrying out a fault diagnostic experiment. The characteristics of the proposed fault diagnostic system were as follows: The necessary knowledge for diagnosing faults was made into each process element. Based on this method, the revision and addition of a knowledge base could be carried out in each element, and the design change of a plant could be flexibly corresponded by only changing the related part of the process flow graph. The estimated results were stored into the working memory, not only faults of an element in which faults resulted could be estimated, but also the fault propagating path could be clarified. 8 refs., 6 figs., 3 tabs.

  2. FY 1999 report on the results of the project supplementary to the New Sunshine Project - Feasibility of coal hydrogasification technology in China. Investigational study of the social adaptability (Feasibility study of the international cooperation - Report of Beijing Research Institute of Coal Chemistry); 1999 nendo New Sunshine keikaku hojo jigyo (Bessatsu, Kokusai kyoryoku kanosei chosa Pekin Bai kagaku kenkyujo) hokokusho. Sekitan suiso tenka gaska gijutsu kaihatsu - Shakai tekigo sei ni kansuru chosa kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    For the purpose of establishing the coal hydrogasification technology which has a possibility of producing high-quality substitute natural gas in quantity and at low cost, an investigational study of the social adaptability was made. In this fiscal year, the following were carried out: outlook of energy supply/demand in China and the problems, natural gas resource and the plan for the use, actual state of the town gas business and the future plan, etc. As a part of the study, Beijing Research Institute of Coal Chemistry, China Coal Research Institute, made a survey under the research contract. As to the general situation of natural gas in China, report was made on the following: present situation of the development of natural gas resource in China, present situation of town gas in large cities of China, present situation and outlook of coal development and utilization in China, assessment of the coal mine area adaptable to coal hydrogasification, etc. In the survey of the area suitable for coal hydrogasification, report was made on the present situation and future of energy supply/demand in Shanghai, Shanxi, Shenfua and Xinjiang, present situation and future of town gas supply, etc. Survey/report were also made on the coal hydrogasification technology and the applicability. (NEDO)

  3. FY 2000 report on the results of the project supplementary to the New Sunshine Project - Feasibility of coal hydrogasification technology in China. II - Final report. Investigational study of the social adaptability (Feasibility study of the international cooperation - Report of Beijing Research Institute of Coal Chemistry); 2000 nendo New Sunshine keikaku hojo jigyo (Bessatsu kokusai kyoryoku kanosei chosa Pekin Bai kagaku kenkyujo) hokokusho. Sekitan suiso tenka gaska gijutsu kaihatsu - Shakai tekigo sei ni kansuru chosa kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    For the purpose of establishing the coal hydrogasification technology which has a possibility of producing high-quality substitute natural gas in quantity and at low cost, an investigational study of the social adaptability was made. In this fiscal year, the following were carried out: natural gas resource and the plan for the use in China, actual state of the town gas business and the future plan, etc. As a part of the study, Beijing Research Institute of Coal Chemistry, China Coal Research Institute, made a survey under the research contract. As a result of the survey, the following was found out: In Xinjiang and Urumchi, Uigur Autonomous Region, there is an abundant coal resource that is suitable for coal hydrogasification, the transportation pipeline of natural gas had been constructed, and public facilities are prepared, and therefore, both cities are suitable for the construction of coal hydrogasification plant. Datong, Shanxi Province, is a largest city of coal production, enables the long-term coal supply for coal hydrogasification, and has a plan for remodeling of old facilities and construction of new facilities for the introduction of natural gas, and therefore, the city is suitable for the construction of coal hydrogasification plant. (NEDO)

  4. Geothermal development promotion survey report. No. 26. Akan region; 1988-1991 chinetsu kaihatsu sokushin chosa hokokusho. No. 26 Akan chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1992-03-01

    The results of surveys conducted in the Akan region, Hokkaido, in fiscal 1988-1990 are compiled in this report. Conducted were a geological/alteration zone survey, geochemical survey, gravity prospecting, electromagnetic surveillance (simplified magnetotelluric method), electric prospecting (Schlumberger method), electric prospecting (mise-a-la-masse method), heat flow rate survey, test boring, geothermal water survey, environmental impact survey, and so forth. The surveys resulted in conclusions mentioned below. Fractures running NE-SW are dominant, and those closely related to prominent geothermal signs are found in the Akan Seibu fault group in the western part of the Akan region. The test boring results show that there are high-temperature zones of 292.1 degrees C, 194.9 degrees C, and 245.9 degrees C. Geothermal fluids were discharged by well N2-AK-7 at a rate of 4.7-4.8 t/h in steam and 0.3-0.4 t/h in neutral SO{sub 4}-HCO{sub 3} type geothermal water. High-temperature steam-dominated geothermal resources are expected to exist deep in the ground in this region, and the area where the Akan Seibu fault group is distributed may be named as a location containing promising geothermal resources. (NEDO)

  5. Regional new energy vision formulation survey for Makurazaki City. Fiscal 2001 report; 2001 nendo Makurazaki shi chiiki shin energy vision sakutei tou chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for elevating people's consciousness of such in Makurazaki City, Kagoshima Prefecture, surveys and studies were conducted concerning the energy demand of the city, the amount of new energy resources in existence, and new energy introduction plans, and then a vision was formulated. The city's energy demand in fiscal 1999 is estimated at approximately 3,206 TJ, with the industrial sector demanding 1,820 TJ, the residential and commercial sector demanding 701 TJ, and the transportation sector demanding 684 TJ. Energy consuming industries in this prefecture are fisheries industry and food industry. Pilot projects for new energy introduction were discussed, which included the introduction of photovoltaic power into the city hall, primary and junior high schools, a school meal providing center, and a city-managed hospital; installation of hybrid power-lighted light at the city hall, along the Kedo river, and in the Hinokami park; introduction of a solar heat utilization system into a nursing home for elderly people; introduction of wind power into education facilities; conversion of waste of cooking oil into diesel fuel; introduction of clean energy vehicles into public service; and new energy projects to be contracted to the private sector. (NEDO)

  6. Report on the project on working out the new energy vision of Hisai City; 2001 nendo Hisai shi chiiki shin energy vision sakutei tou jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Hisai City, Mie Prefecture, an investigational study was conducted of the energy demand structure, existence amount of new energy and project for new energy introduction, and a vision was worked out. The energy consumption amount of the city was 21,500 lorries in the industrial sector, 11,200 lorries in the transportation sector, 2,700 lorries in the business sector and 2,400 lorries in the residential sector in terms of tank-lorry loaded oil. As features, the following were cited: growth in the transportation sector, mostly in automobiles, was great; growth in the electric power consumption in the residential sector was great. As the project for new energy introduction, the following were supposed and studied in terms of the economical efficiency and environmental acceptability: introduction of cogeneration to hospital; introduction of ligneous biomass power generation to hospital; substitution of wood burning boiler for A heavy oil boiler for hot water supply use; introduction of photovoltaic power generation for home use and business use; introduction of solar heat use hot water supply device. (NEDO)

  7. Report on the geothermal development promotion survey. No.B-7. Kuwanosawa area; Chinetsu kaihatsu sokushin chosa hokokusho. No. B-7 Kuwanosawa chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    The paper summed up the results of the geothermal development promotion survey B 'Kuwanosawa area' which was carried out in Yuzawa city, Akita prefecture, from FY 1998 to FY 1999. In the survey, the following were conducted for the comprehensive analysis: geology/alteration zone survey, gravity exploration, electromagnetic exploration, environmental effect survey, well geology survey by drilling structural boreholes of N11-KN-1 and N12-KN-2, cuttings test, temperature log, temperature recovery test, electrical log, water injection test, etc. The geology in the Kuwanosawa area is composed of Pre-neogene period basement rocks, Neogene system and Quaternary system. In this area, there were recognized no gush of geothermal fluids such as hot spring and fumarolic gas and no obvious geothermal manifestation such as high-temperature places and new geothermal alteration zones. Around N12-KN-2, there exists the geothermal water with comparatively high-temperature/high-Cl concentration which is similar to that in the Wasabizawa area, but how it flows is unknown because there was no lost circulation in the depths of the borehole. Around N11-KN-1, there exists the low-temperature/low-Cl concentration geothermal water originating in meteoric water, and therefore, the area can be a rechargeable area. The Kuwanosawa area is regarded as the periphery of the Wasabizawa-Akinomiya geothermal area. (NEDO)

  8. Report on the survey for working out the new energy vision of the Urugi village area; 2001 nendo Urugi mura chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it at Urugi Village, Nagano Prefecture, an investigational study was conducted of characteristics of the natural environment/characteristics of social economy of the village, existence amount of new energy, project for new energy introduction, etc., and a vision was worked out. Urugi Village is 800 meters above sea level and in the riches of the Nature being surrounded by mountains on all sides. The population of the village was 734 as of March 1993, which is decreasing by 14-15/y. The aging rate was very high, 32.3%. As the project for new energy introduction, the following were studied: 10kW class photovoltaic power generation facilities to be installed at Furusato-kan and by the road of Hiraya Pass, 2.5kW class wind power generation facilities to be installed at Hiraya Pass for education of new energy, installation of a 10kW class mini hydroelectric power plant supplying power to the auto-camping site, stockbreeding biomass cogeneration which conducts fermentation of the methane from animal feces and garbage generated in Urugi Village, ligneous biomass cogeneration, etc. (NEDO)

  9. Regional new energy vision for Kobuchizawa Town. Eco-energy town - Kobuchizawa; 2001 nendo Kobuchisawa cho chiiki shin energy vision hokokusho. Eco energy town Kobuchisawa

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for enhancing people's consciousness of such at Kobuchizawa Town, Yamanashi Prefecture, surveys and studies were conducted on the trend of energy consumption in the town, the amount of new energy resources in existence, and new energy introduction projects, and a vision was formulated. The town consumes 635,021 GJ/year in energy, with the industrial sector consuming 34.6%, the transportation sector 24.8%, the residential sector 21.8%, and the commercial sector 18.7%. The energy sources are electric power, kerosene, LPG, gasoline, gas oil, and heavy oil, as named in the descending order in terms of amount. Studies were made about new energy introduction projects, which included the use of photovoltaic power for outdoor lights, primary schools, nursery schools, the flower park (Fiore Kobuchizawa), mountain huts, and for the Kannondaira sightseeing spot; mini-scale hydroelectric power generation for the maintenance of the Otaki spa and existing water channel utilities; wind power generation for improvement on mountain huts; introduction of clean-energy vehicles; introduction of cogeneration (natural gas); and a business for effectively utilizing biomass. (NEDO)

  10. Report of the researcher exchange promotion project on the environmental issues in the Asia-Pacific region; Asia/Taiheiyo chiiki kankyo mondai kenkyusha koryu sokushin jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    Proposals have been made for the establishment of a network (ETERNET-APR) linking those involved in the research and development of environmental technology in the Asia-Pacific region in order to limit the environmental impact of industrial activity. By pursuing active exchanges of information and personnel, researchers in environmental technology in the Asia-Pacific region have been making serious efforts to establish such a network. This fiscal year, the Internet Web site of the ETERNET-APR has been created using the data collected to date. This database includes information on some 350 researchers and 200 research projects from seven countries. The first international symposium was successfully held at Environmental Research Institute of Chulalongkorn University in Thailand (ERIC), hosting 200 environmental researchers from 10 countries in the Asia-Pacific region. Tripartite sister laboratories ties among the National Institute for Resources and Environment (NIRE) and three Korean laboratories were forged. The sister laboratory project between ICETT and ERIC is also proving effective. These successes prove that intraregional joint research, the objective of ETERNET-APR, has begun to take shape in this year

  11. Report on investigation new energy vision establishment in Zao Town area in fiscal 2000; 2000 nendo Zaocho chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A new energy vision was established in the Zao Town area in Miyagi Prefecture. The population of Zao Town is 14,240 persons as of 2000, continuing the slow decreasing trend since 1986. The energy consumption is accounted for by the industrial department at about 50% of the total consumption, followed by the transportation department at about 30%, and the consumer department at about 20%. Discussions on the new energy utilization possibility revealed 30,905 MWh annually by photovoltaic power generation, and 34,630Gcal annually by solar heat utilization. Discussions were also given on power wind generation, small to medium hydropower generation, utilization of hot spring heat, and others. The new energy introducing project includes introduction of photovoltaic power generation into such public facilities as the public hall and athletic park, private sector operated houses and business facilities, introduction of wind power generation into the surrounding areas of the Zao Heart Land and skiing areas, snow melting utilizing hot spring heat, conversion of the automobiles used in the official organizations into clean energy fueled automobiles, installation of bio-mass plants, illumination of parks and streets utilizing photovoltaic and wind power generation systems, and micro hydropower generation in small to medium size rivers. (NEDO)

  12. Report on the geothermal development promotion survey. No.34. Kaminoyu/Santai area; Chinetsu kaihatsu sokushin chosa hokokusho. No. 34 Kaminoyu Santai chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-03-01

    The paper summed up the results of the geothermal development promotion survey 'Kaminoyu/Santai area' which was carried out at Yakumo town, Yamakoshi county, and Mori town, Kayabe county, Hokkaido, from FY 1990 to FY 1992. In the survey, the following were conducted for the comprehensive analysis: surface survey such as geology/alteration zone survey, geochemical survey and gravity exploration, test to induce jetting of geothermal fluids by drilling 7 boreholes, temperature/pressure log, etc. The geological structure of this area is featured by the uplift structure/caldera structure and the anticline structure. In the wide-area geothermal system, it is thought that the low-temperature seawater permeates underground by mixture with meteoric water, is heated, and forms the deep geothermal water. It is thought that the hot spring water in this area is stored in the hot spring reservoir by mixture with the surface water, and it is gushing. In the area, the region which is regarded as favorable as passage/storage place of geothermal fluid is a region of 1.5km width extending linearly in the E-W direction from the Nigorikawa basin to Kaminoyu. Further, it is thought that fractures were unfavorably developed in the Santai/Kaminoyu area. (NEDO)

  13. Regional new energy vision for Kakegawa City. Formulation survey report; 2001 nendo Kakegawa shi chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for enhancing people's consciousness of such at Kakegawa City, Shizuoka Prefecture, surveys and studies were conducted involving the energy demand of the city, the amount of new energy resources in existence, and new energy introduction projects, and a vision was formulated. The city consumes approximately 11,638 GJ/year, with the industrial sector consuming 4,896 GJ, the residential/commercial sector 3,608 GJ, and the transportation sector 3,134 GJ. Energy types are electric power, gas oil, heavy oil, gasoline, kerosene, LPG, and town gas, as listed in the descending order in terms of consumption rate. The amount of carbon dioxide resulting from the consumption is estimated at 673,214 t-CO2. Projects for new energy introduction were studied, which involved photovoltaic power generation for the general-purpose gymnasium, kindergartens, the school meal center, and meeting places; introduction of solar heat utilization systems into public facilities; financial support system for the purchase of solar heat utilization apparatuses; refuse-fueled power generation and heat utilization at a new cleaning center; utilization of biogas from garbage and stock raising waste; utilization of woody resources; clean-energy vehicles; and so on. (NEDO)

  14. Regional new energy vision formulation project for Ukiha Town. Fiscal 2001 report; 2001 nendo Ukiha machi chiiki shin energy vision sakutei tou jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for enhancing people's consciousness of such in Ukiha Town, Fukuoka Prefecture, surveys and studies were conducted involving the amount of energy needed by the town, the amount of new energy resources in existence, and new energy introduction projects, and a vision was formulated. The energy consumption of the town is estimated at 158,886 drums/year in terms of crude oil - 56.2% in electric power, 19.5% in gasoline, 10.5% in gas oil, 6.9% in kerosene, 4.2% in fuel oil A, and so forth. Projects for introducing new energies were studied, which included the introduction of photovoltaic power systems into public facilities, gymnastic halls, and comprehensive welfare centers; introduction of a large wind power system (1,500 kW) in the vicinity of the Michinoeki roadside service spot; installation of a power plant fueled by woody biomass (scrap wood, saw dust, and the like); introduction of clean energy vehicles for official use; installation of photovoltaic/wind hybrid power-lighted street lamps in the shopping area; installation of light traps for agriculture burned by photovoltaic power; and financial aids for encouraging the installation of photovoltaic power generation systems in general residences. (NEDO)

  15. Regional new energy vision formulation for Kadogawa Town, Miyazaki Prefecture. Report; 2001 nendo Miyagi ken Kadokawa cho chiiki shin energy vision sakutei tou jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for elevating people's consciousness of such in Kadogawa Town, Miyazaki Prefecture, surveys and studies were conducted concerning the energy demand in the town, the amount of new energy resources in existence, and new energy introduction plans, and then a vision was formulated. The town's energy demand is estimated to be 1,197.4-million MJ/year, which includes 44.1% for the transportation sector, 22.9 for the residential sector, 22.7% for the industrial sector, and 10.3% for the people's livelihood-related business sector. The energy demand is covered 62.7% by oil-based fuels, 22.7% by electric power, and 13.4% by LP gas. Projects discussed for new energy introduction included the introduction of photovoltaic power into the town office, primary schools, and a comprehensive workers' welfare center; introduction of a solar hot water supply system and hybrid power-lighted street light into a seaside park; introduction wind power into Motoyama Park; mini-scale hydroelectric power on the Isuzu river; introduction of clean energy vehicles into the town office; power generation fueled by methane gas from livestock excrement; and introduction of natural gas fueled cogeneration into a resort house. (NEDO)

  16. Regional new energy vision formulation survey for Ochi Town. Report; 2001 nendo Ouchi cho chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for elevating people's consciousness of such in Ochi Town, Saga Prefecture, surveys and studies were conducted involving the amount of energy needed by the town, the amount of new energy resources in existence, and new energy introduction programs, and a vision was formulated. The demand for energy of the town is estimated at 613,575 times 10{sup 9} J, with the industrial sector responsible for 47.7%, the transportation sector for 26.6%, and the residential and commercial sector for 25.7%. Electric power, heavy oil, gas oil, gasoline, and kerosene are demanded, named in the descending order in terms of magnitude of demand. Key projects were discussed for new energy introduction, which included the introduction of photovoltaic power into a culture exchange center; nighttime illumination of Mikaeri Waterfall by micro-scale hydroelectric power; installation of a water purification system for the Ikisa Dam using photovoltaic power and micro-scale hydroelectric power; introduction of photovoltaic power into a comprehensive sports park and the Ochi Junior High School; and large-scale agricultural production and processing plant operation using photovoltaic power. (NEDO)

  17. Plans for promoting district energy-saving visions for City of Wakkanai; Wakkanai-shi chiiki sho energy vision suishin keikakusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    The district energy-saving visions and promotion plans therefor are drawn for City of Wakkanai, to promote energy saving and abatement of gas emissions which cause global warming. The conception of the energy-saving introduction project includes introduction of co-generation systems in the facilities of pivotal functions in the city center, and energy-saving systems in a municipal heated pool and public hot spring facilities. The facilities of pivotal functions in the city, e.g., the municipal office buildings, municipal hospital and all-around culture center of the city are supplied with heat in a centralized manner from the energy center, and the energy-saving effect is expected when co-generation systems are introduced in the energy center. The study results indicate that introduction of the co-generation systems in the energy center has a potential energy-saving effect of 2,400Gcal/y. The energy-saving effects are also analyzed for each of the municipal office buildings, municipal hospital, all-around culture center, municipal heated pool and public hot spring facilities, including those potentially realized by improving illumination and air supply/discharge facilities. (NEDO)

  18. Report on the geothermal development promotion survey. No.36. Mt. Amemasudake area; Chinetsu kaihatsu sokushin chosa hokokusho. No. 36 Amemasudake chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-03-01

    The paper summed up the results of the geothermal development promotion survey 'Mt. Amemasudake area' which was carried out at Akaigawa village, Yoichi county, Hokkaido, from FY 1991 to FY 1994. In the survey, the following were conducted for the comprehensive analysis: surface survey such as geology/alteration zone survey, geochemical survey, gravity exploration, electromagnetic exploration and electric exploration, core test by drilling 5 boreholes, test to induce jetting of geothermal fluids, measurement of in-borehole temperature/pressure, survey of geochemical properties of geothermal water. As to the fracture system in this area, the Amemasudake fault and the Amemasuzawa fault are especially important, and it was assumed that these faults had relation to the present geothermal distribution. It is thought that structural conditions of geothermal reservoirs are fractures in basement rocks. As a result of the borehole survey, it was indicated that the center of the high-temperature part with a temperature of 250 degrees C or more was in the boundary zone southeast of this area at a level of 500m below sea level. The scale, which reached about 3km both in east/west and north/south, is almost the same scale as that of the neighboring Toyoha area where great potentiality of the geothermal development is expected. (NEDO)

  19. Subsurface interpretation based on geophysical data set using geothermal database system `GEOBASE`; Chinetsu database system `GEOBASE` wo riyoshita Kakkonda chinetsu chiiki no chika kozo kaiseki

    Energy Technology Data Exchange (ETDEWEB)

    Osato, K; Sato, T; Miura, Y; Yamane, K [Geothermal Energy Research and Development Co. Ltd., Tokyo (Japan); Doi, N [Japan Metals and Chemicals Co. Ltd., Tokyo (Japan); Uchida, T [New Energy and Industrial Technology Development Organization, Tokyo, (Japan)

    1996-05-01

    This paper reports application of a geothermal database system (GEOBASE) to analyzing subsurface structure in the Kakkonda geothermal area. Registered into the GEOBASE to analyze specific resistance structure in this area were depth information (well track and electric logging of existing wells), three-dimensional discretization data (two-dimensional analysis cross section using the MT method and distribution of micro-earthquake epicenters), and two-dimensional discretization data (altitude, and depth to top of the Kakkonda granite). The GEOBASE is capable of three-dimensional interpolation and three-dimensional indication respectively on the three-dimensional discretization data and the depth information table. The paper presents a depth compiling plan drawing for 2000 m below sea level and an SE-NE cross section compiling cross sectional drawing. The paper also indicates that the three-dimensional interpolation function of the GEOBASE renders comparison of spatial data capable of being done freely and quickly, thereby exhibiting power in the comprehensive analysis of this kind. 3 refs., 8 figs., 2 tabs.

  20. Subsurface geology by shallow seismic reflection survey and microgravity survey in Kobe area; Senso hanshaho danseiha tansa oyobi seimitsu juryoku tansa ni yoru Kobe chiiki no chika chishitsu

    Energy Technology Data Exchange (ETDEWEB)

    Endo, H; Makino, M; Murata, Y; Watanabe, S [Geological Survey of Japan, Tsukuba (Japan)

    1996-05-01

    This paper reports discussions on subsurface structure of the Kobe area by means of microgravity survey. A Bouguer anomaly distribution graph was obtained (which is close to a near straight line) by measuring the gravity at measurement points with an interval of about 25 m along a traverse line (with a total length of about 1.7 km) in an approximately NNW-SSE direction crossing the downtown of the city of Kobe. Viewing the residuals to a straight line linking the values at both ends of the traverse line revealed that the gradient in the Bouguer anomaly distribution changes as if it is bent at a point near the center of the traverse line as a boundary. Deriving the residuals from fine changes seen in a distribution graph for the residuals made clear that this residual distribution has two discontinuous points. The paper indicates that the bending point in the former case corresponds to a reverse fault in a base bed of a flat land as compared with the result of model calculation on the base structure and gravity distribution. The discontinuous points in the latter case agree with positions of fault distribution derived by a reflection elastic wave survey. 6 refs., 4 figs.

  1. Fiscal 2000 survey report on regional new energy vision formulation for Kawanabe town, Kagoshima Prefecture; 2000 nendo Kawabecho chiiki shin energy vision sakutei nado chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    A regional new energy vision has been formulated for Kawanabe town, Kagoshima Prefecture, to be participated in by inhabitants and beneficial to both natural environment protection and inhabitants. The results of the survey are described in six chapter which involve (1) the basic philosophy of vision formulation, (2) outline of new energy, (3) environmental characteristics and townspeople's awareness, (4) energy situation and the amount of new energy existing in Kawanabe town, (5) regional new energy vision for Kawanabe town region, and (6) future tasks. After a study of the existing amount of new energy, energy demand, and people's requests, it is concluded that photovoltaic power generation, wind power generation, hot spring heat utilization, small scale water energy, and refuse-derived energy be the types to be preferably introduced into the town. Projects are established and concrete plans are prepared thereunder, which concern the reinforcement of public relations and enlightening activities, installation of a wind power system as one of enlightening activities, study of Kawanabe town's own subsidy system for encouraging the introduction of photovoltaic power systems and solar heaters for domestic use, and the introduction of photovoltaic power systems into public facilities such as school houses. In conclusion, it is hoped that, since financial difficulties will be a strong factor to impede new energy introduction, the subsidy system will be a solid one. (NEDO)

  2. FY 2000 report on the analytical survey of the basic data by area; 2000 nendo chosa hokoku. Chiiki betsu kiso data bunseki chosa

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    From a viewpoint of the spread of energy conservation technology, etc. that Japan owns, following the previous fiscal year, data collection/study were made of 51 countries and 1 area including 49 countries (17 Asian developing countries, 13 Middle East countries and 19 countries in East Europe/Former U.S.S.R.) and 2 countries (Japan/the U.S.) and 1 area (the EU) that were added for comparison with developed countries. Items for study were the general situation, economic/trade indicators, administration/general situation, energy situation, economic/technical cooperation from Japan, implementing situation of NEDO's model project, AIJ implementing situation in each country, etc. To keep the continuity, data were numerically obtained from the same data source as that used in the previous fiscal year as a rule. The input data were made as consistent in each item as possible, and the same table was obtained from a single data source as a rule. The data in the previous fiscal year were reviewed, and the data were adopted from which data on as many countries as possible could be acquired through the same data source. (NEDO)

  3. Report on the project for working out the new energy vision of the Iwasaki village area; 2001 nendo Iwasaki mura chiiki shin energy vision sakutei tou jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it at Iwasaki Village, Aomori Prefecture, an investigational study was conducted of the energy demand of the town, existence amount of new energy, introduction of new energy, etc., and a new energy vision was worked out. The population of Iwasaki Village was 2,845 as of October 2000, which decreased approximately 30% in the past 20 years. The energy demand of the whole village was about 280,000 GJ in amount (about 7,202 kL/y in crude oil conversion), consisting of kerosene, light oil, heavy oil, gasoline, electric power and LPG. As viewed by sector, the rate of energy demand in the commercial/residential sector was comparatively high. As the project for new energy introduction, the following were studied: wind power generation around the Yamamori plain/Iwasaki village office; snow melting system using soil heat exchanger on the road in front of elementary school, photovoltaic power generation for public facilities (elementary and junior high schools/house of recreation and relaxation for old people/day nursery)/Tsugaru Semi-national Park/general houses; wave power generation at the Iwasaki fishing port; introduction of clean energy vehicles to public facilities; utilization of ligneous biomass energy, etc. (NEDO)

  4. Let People Bathe in Clean Energy. Regional new energy vision for Matsuyama Town; 2001 nendo Matsuyama machi chiiki shin energy vision. Toumeinal energy wo sosoide

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for enhancing people's consciousness of such at Matsuyama Town, Yamagata Prefecture, surveys and studies were conducted involving the amount of energy needed by the town, the amount of new energy resources in existence, and new energy introduction projects, and then a vision was formulated. The town demands 120,407-million kcal/year in energy comprising 56.8% from oil based fuels, 39.2% from electric power, and 4.1% from LP gas. As for consumption, 35.6% is consumed by households, 28.9% by industries, 21.3% by transportation, and 14.2% by commerce. The amount of carbon dioxide due to the consumption is estimated at 28,000 t-CO2/year. Key projects for new energy introduction were discussed, which included an eco-town project for introducing photovoltaic power generation systems, passive solar heat utilization systems, clean energy vehicles, and so forth, into public facilities; an eco-agriculture project for utilizing wind power generation and livestock excreta energy; an eco-park project for exhibiting new energies to the public; and an eco-school pilot model project. (NEDO)

  5. Fiscal 1999 technical survey report. Basic survey of prioritized fields in South Asia (India); 1999 nendo Minami Asia chiiki (Indo) juten bun'ya kiso chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    For working out and proposing project themes optimum for the popularization of technologies for effectively utilizing energy in India, a basic survey was conducted into India's energy policy, trend of supply and demand, steelmaking industry, papermaking industry, petrochemical industry, and chemical fertilizer making industry. India in fiscal 1997 consumed 374-million tons of energy in terms of oil, 30% in oil and 63% in coal. Activities were vigorous for the development of oil substitutes such as photovoltaic and wind power. The steelmaking industry that produced 22.12-million tons in fiscal 1996 was found to suffer from a reduced operating rate due to aging of facilities and from poor infrastructures such as harbor and railway facilities wanting maintenance. It was inferred that energy conservation facilities and technologies of Japan's steelmaking industry would work effectively. The cement making industry was found modernized, but faced the problem of the high cost of electric power. The papermaking industry was busy enhancing its own production capability. The fertilizer making industry ranked third in the world in terms of production, but the energy consumption rate at its ammonia plant was too high. The petrochemical industry was well developed, with Japan's technologies incorporated into it in the design stage. (NEDO)

  6. - From point, line to plane - . New energy vision at Osafune Town area; 2001 nendo Osafune cho chiiki shin energy vision (Ten sen soshite men he)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    With an objective of accelerating introduction of and uplifting consciousness on new energies at Osafune Town in Okayama Prefecture, investigations and discussions were given on the energy consumption structure, status of new energies available, and the new energy introduction projects for the town, thus a new energy vision was established. The estimated amount of energy consumed in Osafune Town is 1,443,467 GJ/year. By departments, the industrial department accounts for 41%, the transportation department for 40%, the civil household department for 13% and the civil business department for 6%. Energy types are accounted for by petroleum at 81%, and electric power at 19%. The important new energy introduction projects discussed as the first step include 'Cooperative projects by residents and the administration', and 'Wide area introduction of new energies' into public facilities, as the second step, 'New energy networking' targeted at mutual coordination, 'Information network' for new energies and energy saving, and as the third step, 'Acceleration of proliferation at general levels' beginning with housing, and 'Creation of green energy experiencing park' at the Osafune Beautiful Forest. (NEDO)

  7. Regional new energy vision formulation project for Takarabe Town. Report; 2001 nendo Takarabe cho chiiki shin energy vision sakutei tou jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for elevating people's consciousness of such in Takarabe Town, Kagoshima Prefecture, surveys and studies were conducted concerning the energy consumption profile of the town, the amount of new energy resources in existence, and new energy introduction plans, and then a vision was formulated. The town's energy consumption is estimated at 879.350-million MJ/year, with the transportation sector responsible for 46.6%, the industrial sector for 23.0%, the residential sector for 18.7%, and the people's livelihood-related business sector for 11.7%. Oil-based fuels occupy 70.4%, electric power 18.9%, and LP gas 8.4%. New energy introduction projects were studied, which included the introduction of photovoltaic power into the town office; photovoltaic power and hybrid power-lighted light for an oasis-on-the-road project and school facilities; introduction of cogeneration, wind power, and photovoltaic power into a hot spa health center and a water-friendly park; small-scale hydropower in the Okawara valley; wind power generation on Mount Shiraga and Jingaoka; introduction of clean energy vehicles for official use; and compost manufacturing and power generation using livestock waste. (NEDO)

  8. New energy vision of the Tono City area; 2001 nendo Tonoshi shin energy vision. Chiiki shin energy vision sakutei tou jigyo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    As a leading plan to reconsider the resource consumption in large quantities and to change the society to a circulation type, a new energy vision of the Tono City area was worked out in Tono City, Iwate Prefecture. In this vision, FY 2002 was set as the first fiscal year, and FY 2010 as the target year. From the comprehensive judgement based on the objective availability, technical evaluation, civic needs, etc., biomass energy, wind energy, solar energy, etc. were made the main energy in new energy introduction. As model projects, studied were the introduction of solar energy utilization facilities to Kamigo Elementary School, introduction of the photovoltaic power generation to Aozasa Nursery School and disaster prevention base facilities, project on the wind power generation using large wind turbines, biomass alcohol plant, wood pellet production, etc. The target amount of new energy introduction was 2,640 kW in photovoltaic power generation, 19,000 kW in wind power generation, etc. The CO2 reduction amount including that in heat utilization, clean energy car, etc. was 41,374 t-CO2/y. (NEDO)

  9. Regional new energy vision formulation project for Kanai Town. Fiscal 2001 report; 2001 nendo Kanai machi chiiki shin energy vision sakutei tou jigyo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For promoting the introduction of new energy and for enhancing people's consciousness of such at Kanai Town, Niigata Prefecture, surveys and studies were conducted involving energy demand of the town, the amount of new energy resources in existence, and new energy introduction projects, and a vision was formulated. The town demands 541,062 GJ/year in energy, with the residential/commercial sector requiring approximately 46%, the industrial sector approximately 30%, and the transportation sector approximately 25%. The energy types are kerosene, heavy oils, gas oil, electric power, gasoline, and LPG, mentioned in the descending order in terms of magnitude of requirement. The resultant amount of carbon dioxide is estimated at 11,449 t-C. New energy introduction projects were discussed, which included photovoltaic power generation for primary and junior high schools, the town office, and the Dorin stock farm; a solar heat utilization system for the school meal center; wind power generation for Hometown Kimpoku, primary schools, and the Dorin stock farm; mini-scale hydroelectric power generation on the Shimpo river; use of forestry biomass (pellet stoves) at nursery schools; introduction of clean-energy automobiles for public use; and the introduction of cogeneration into Sado Hospital. (NEDO)

  10. Report on results of the survey for drawing new energy visions for the Town of Shiranuka district; Shiranukamachi chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The new energy visions are drawn for Town of Shiranuka. Utilization of solar energy is at the top of the prospective new energy reserves for the town, followed by utilization of heat of wastes, biomass energy (in the stock raising industry), and photovoltaic power generation. The expected recoverable new energies account for approximately 35% of the total annual energy consumption of the town. The basic guidelines for introduction of new energies are drawing the priority projects which place importance on environmental education and bringing up talented persons, development of the soft policies for supporting the citizens and related entrepreneurs, and introduction of new energies for promoting new industries. The priority projects include hard ones, e.g., foundation of Eco-school Shiranuka, Eco-park Kushiro/Shiranuka (biomass energy supply center) and Koima new energy park, and soft ones, e.g., establishment of the new energy grand prize awarded by PR brochure ''Shiranuka'', foundation of the Shiranuka fund for introduction of new energies, construction of a Shiranuka Citizens' power station, and rent-a-car project for eco-car test runs. (NEDO)

  11. Report on the geothermal development promotion survey. No.B-6. Mt. Tsujinodake area; Chinetsu kaihatsu sokushin chosa hokokusho. No. B-6 Tsujinodake chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The paper summed up the results of the geothermal development promotion survey B 'Mt. Tsujinodake area' which was carried out at Yamagawa town, Ibusuki county, Kagoshima prefecture, from FY 1997 to FY 1999. In the survey, the following were conducted: surface survey such as geology/alteration zone survey, fluid geochemical survey, gravity exploration and electromagnetic exploration, and geothermal fluid survey by drilling 4 structural testing boreholes. In the Mt. Tsujinodake area, active fumarolic activities are still now continuing around the Unagi hot spring in the north, and the acidic alteration zone is distributed in the peripheral surface area. In Borehole N11-TD-2 in the north, it is assumed that there exists the hydrothermal bed of a temperature of approximately 230 degrees C in the depth of 400-800m, which is comparatively shallow, and that the stored geothermal water flows laterally. In the central area, a hydrothermal bed of a temperature of approximately 130 degrees C was confirmed in N11-TD-1, but it is judged that chances of the existence of high temperature hydrothermal bed are not very good. In N11-TD-3 in the south, the existence of promising heat sources is expected in the deeper part since the remarkable alteration was seen in the depth of 1,435m or deeper, and the sharp rise in temperature was recognized. (NEDO)

  12. Regional new energy vision formulated for Murakami City; 2001 nendo Murakami shi chiiki shin energy vision sakutei tou jigyo. Murakami shi shin energy puran

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For promoting the introduction of new energy and for enhancing people's consciousness of such at Murakami City, Niigata Prefecture, surveys and studies were conducted involving energy consumption in the town, the amount of new energy resources in existence, and new energy introduction projects, and a vision was formulated. The annual energy consumption of the city is estimated at 78,000 kL in terms of oil. The residential/commercial sector consumes 24,000 kL, the industrial sector 28,000 kL, and the transportation sector 26,000 kL, with the three sectors demanding similar amounts. The carbon dioxide emitted by the sectors is estimated at 223,000 t-CO2/year. New energy introduction projects were discussed, which included hot water supply, heating, road heating, and power generation, all these utilizing heat from the Senami hot spa; biogas fueled power generation and BDF (bio-diesel) bus operation in the Senami hot spa district; the introduction of a hybrid wind power/photovoltaic power generation system into Ogata Seaside Park; the introduction of a mini-scale hydroelectric power generation system into the Kamikaifu district; a large-scale wind power generation business at the Iwafune district; the introduction of wind power and photovoltaic power generation facilities into primary and junior high schools; the use of clean energy vehicles for official use; and so forth. (NEDO)

  13. Report on new energy vision establishment project in Higashi Naruse Village area; Higashinarusemura chiiki shin energy vision sakutei nado jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A new energy vision was established in the Higashi Naruse Village in Akita Prefecture. The population of the village is continuing the decreasing trend since 1950 to 3,390 persons as of 2000. The annual energy demand in this village is about 320,000 GJ, accounted for by the consumer department at about 40%, the transportation department at about 36% and the industrial department at about 24%. The carbon dioxide emission amount therefrom is estimated 6,300 t-C annually. The expected usable quantity of new energies in the village would be available, in the decreasing order, in the wind power as the most promising source, forestry bio-mass, snow cold heat energy, agricultural bio-mass, and solar beam (heat). The total quantity would be 514,330 GJ annually in heat utilization, and 85,305 MWh in electric power. The new energy introducing project includes introduction of photovoltaic and wind power generation into Kurikoma Mountain Villa, introduction of wind power generation into the Junes skiing area and Oyanagi Natural Park, introduction of photovoltaic power generation and heat utilization into primary and middle schools, introduction of clean energy fueled automobiles into the village office, and introduction of photovoltaic power generation into general houses. (NEDO)

  14. Report on the FY 1999 feasibility study for the international joint research in the Asian area. 6; 1999 nendo Asia chiiki kokusai kyodo kenkyu kanosei chosa hokokusho. 6

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    The paper surveyed the situation of the recent economic crisis, social circumstances, trends of energy policies, etc. in Asian countries. The energy consumption in Asian countries mainly including ASEAN countries and China is expected to show a large growth hereinafter together with a rapid economic growth on a long-term basis. Further, the harmony with the environmental problem has been requested. This problem is important also to Japan from a viewpoint of the regional response to the stabilized energy supply/environmental problems. The response to global environmental problems in these countries and the promotion of development/spread of new energy/energy conservation are strongly desired. For the spread of these technologies, it is effective to actually construct/operate/maintain plants under the joint research with Japan from viewpoints of personnel training, technology improvement, PR effects, etc. It is helpful also for Japan from the aspect of technology development such as data acquisition. It is necessary to accurately grasp the needs and technical levels of the other country in the case of carrying out the joint research. The survey was made about 7 countries including Thailand, Indonesia, Malaysia, the Philippines, India, China and Vietnam. (NEDO)

  15. Fiscal 1999 technical survey report. Basic survey of prioritized fields in Central Asia; 1999 nendo Chuo Asia chiiki juten bun'ya kiso chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    For working out and proposing project themes optimum for the popularization of technologies for effectively utilizing energy in four countries in Central Asia (Kazakhstan, Uzbekistan, Turkmenistan, and Azerbaijan), basic surveys were conducted into their energy policies, trends of supply and demand, electric power industry, metal refining industry, and cement making industry. Common to the four nations were that they were rich in energy resources (natural gas, oil, and coal) but that they failed to utilize them effectively despite of an increase in the yields. Incomplete infrastructures and delay in the implementation of energy measures attracted attention. A higher priority was found attached to the upgrading of and investment in structures, renewal of facilities, and the intensification of management. The basic industries wanted the modernization of their facilities and processes. As for electric power consumption, Kazakhstan consumed 53.0-billion kWh, Uzbekistan 39.0-billion kWh, Turkmenistan 4.4-billion kWh, and Azerbaijan 16.4-billion kWh. In Uzbekistan, where the nonferrous metal refining industry led its national economy, the energy consumption rate was found to be quite high. There was a decrease in production in the cement making industry. (NEDO)

  16. Fiscal 2000 survey report on regional new energy vision formulation for Kito village; 2000 nendo Kitoson chiiki shin energy vision sakutei nado chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A regional new energy vision has been formulated for Kito village, Tokushima Prefecture, in the hope that it will trigger off an effort to build a mentally affluent community. Kito village is a 2,000-strong village situated in a mountainous region, and consumed 1.3 times 10{sup 5} GJ in energy in fiscal 1999. The results of activities conducted in this connection are summarized in four chapters which are (1) the outline of survey, (2) local characteristics, (3) existing amount of new energy, and (4) plans for promoting new energy introduction and energy conservation. Discussed as plans for promoting new energy introduction and energy conservation are a business of commercialization feasibility survey, plot to construct a natural energy operated school building, and a plan for promoting energy conservation. Discussed in relation to commercialization feasibility survey are a small scale hydroelectric power generation utilizing a sand-trap dam, small scale hydroelectric power generation utilizing waste water from a fish farm, regional heat supply business utilizing ligneous biomass, manufacture of ligneous pellet fuel, and so forth. In relation to the promotion of energy conservation, a drive will be launched to positively bring photovoltaic power generation into daily life. (NEDO)

  17. Report on results of the survey for drawing new energy visions for the Town of Hamanaka district; Hamanakacho chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    The new energy visions are drawn for Town of Hamanaka, Hokkaido. The town had a population of 7,866 in 1995, which compares with 11,915 in 1960, aging as a whole with reduced number of new born babies. The town has been holding the Japan's top town in tangleweed production and core site in the Kushi-Ne dairy belt. Biomass energy (excreta and woody resources) is at the top of the prospective new energy reserves for the town, which total 88,741Gcal/y, followed by utilization of solar energy and heat exhausted from garbage incineration. The priority projects selected for introduction of new energies include a biogas plant which produces organic compost and biogas energy by anaerobic fermentation of excreta discharged from domestic animals, raw garbage and fishery wastes; system for utilizing waste heat from a stoker type incinerator burning domestic garbage, which satisfies the dioxin emission standard; and system for utilizing low-temperature heat including energy of snow and ice, which uses only a limited extent of the conventional energy and free of chlorofluorocarbon gases. (NEDO)

  18. Fiscal 2000 survey report on coal exploitability overseas. Rajah area, New Zealand; 2000 nendo kaigaitan kaihatsu kanosei chosa hokokusho. New Zealand Rajah chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    A geological structure survey was conducted in the Rajah mining field, New Zealand, to study coil exploitability there. Conducted were the drilling of five boreholes (and three more by Solid Energy Corporation, New Zealand), surface exploration, surface survey, borehole core specimen examination, coal specimen analysis, and the like. It was found that the coal from this field includes 3-5% of ash and 0.3-0.4% of sulfur and therefore is excellent as coal for coke making. There are three working coal beds, 1.7m at the thinnest section and approximately 7m at the thickest section. It is concluded, on the assumption that sections not less than 2m in thickness are productive, that there are approximately 15-million tons in reserve. Since the coal beds are inclined and very variable in thickness, it is believed that hydraulic coal mining will be the best method to use. Water for hydraulic coal mining is sufficiently supplied from rivers in the neighborhood. The field is located near Rapahoe which serves as depot for coal produced in this area, is equipped with facilities for shipment by rail, and has various industries necessary for supporting coal mine operation. Coal is shipped from Greymouth Port in the area aboard 8,000-tons class barges. (NEDO)

  19. Report on investigation of new energy vision planning at Tomi Village; 2001 nendo Tomi son chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    With an objective of accelerating introduction of and uplifting consciousness on new energies at Tomi Village in Okayama Prefecture, investigations and discussions were given on the energy demand, quantity of new energies available, and the new energy introduction projects for the town, thus a new energy vision was established. The amount of energy demand in Tomi Village is 64,261 GJ/year in fiscal 1999. By departments, the transportation department accounts for 45.2%, the consumer department for 28.3% and the industrial department for 26.5%. Energy types are accounted for by petroleum at 87.5%, and electric power at 12.5%. The new energy introduction projects have discussed introduction of photovoltaic power generation system and solar heat hot water making system into nursery schools, primary and middle schools, the public hall, the Tomi Communication Center, the village office, and clinics, small size wind power generation system into primary and middle schools, ligneous bio-mass combustion system and middle size wind power generation system into the camping site, low-drop electric power generation system into Shiraga Valley, street lights powered by photovoltaic and small power wind generation systems, and clean energy fueled automobiles for public use. (NEDO)

  20. New energy vision of the Sen'nan city area. Outline; 2001 nendo Sen'nan shi chiiki shin energy vision (Gaiyo ban)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Sen'nan City, Osaka Prefecture, a new energy vision was worked out, and a version of the outline was made. The policy on new energy introduction was described as follows: to make a great use of solar energy; to promote the spread of low-emission car and other new energy; to construct a system for spread that is connected to cooperation with citizen and education/enlightenment. Concretely, the following were cited: introduction of photovoltaic power generation to the city office/elementary school/junior high school; study of preferential treatment for introduction of solar energy; introduction of low-emission car to official vehicle; study of preferential treatment for introduction of low-emission car; promotion of car sharing by low-emission car; introduction of natural gas cogeneration and fuel cell to the city office; installation of street light using small wind power generation; potential study of small- and medium-size power generation; support for class for new energy experience at elementary/junior high school; potential study of utilization of biomass energy such as bamboo charcoal; study of preferential treatment for new energy to be given to citizen and enterprises in the city; construction/support of energy utilization system using waste, etc. (NEDO)

  1. New energy vision at Sabae City. Toward realization of 'environmental international city'; 2001 nendo Sabae shi chiiki shin energy vision. Kankyo kokusai toshi Sabae no jitsugen ni mukete

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    With an object of accelerating introduction of and uplifting consciousness on new energies at Sabae City in Fukui Prefecture, a new energy vision was put into order based on the result of the initial stage investigation having been performed in the previous fiscal year. Solar light and solar heat energies will be introduced positively into public facilities, and at the same time works will be implemented to accelerate the proliferation thereof into general households. The target of introduction by the year 2010y is set to 2,600 kW by photovoltaic power generation, and the introduction thereof is planned to schools, nursery schools, municipality operated houses, and unattended facilities. With regard to solar heat water warmer, the present proliferation rate of about 7.3% will be raised to 15%. Regarding wastes generated from industrial areas, efficient energy extraction by thermal recycling is intended on the assumption of suppression of wastes generation, their re-use, and recycling. Systems may include the bio-gas system, cogeneration, and recovery of energies from plastics wastes. For acceleration of proliferation of new energies in general, promotion of introduction will be achieved on mini-wind power generation, small hydroelectric power generation, and fuel cell automobiles. (NEDO)

  2. MT survey in the Minaminoshiro oil field; Minaminoshiro chiiki ni okeru MT ho ni yoru sekiyu tanko (sanjigen MT ho chosa no kento). 1

    Energy Technology Data Exchange (ETDEWEB)

    Matsuo, K; Minegishi, M [Japan National Oil Corp., Tokyo (Japan). Technology Research Center

    1997-10-22

    Preliminary experiment, investigation, and study are conducted prior to an experimental 3-D MT (magnetotelluric) survey planned to be carried out in the Minaminoshiro district, Akita Prefecture. In the preliminary investigation, MT measurements were performed at nine locations. It was anticipated that national highways, waste treatment plants, high-voltage power lines, and railways in presence in the district would act as noise sources. Although the impact of such noise was detected in the single site treatment result, yet it was found that it would be mostly eliminated by use of the remote reference treatment. It was learned that the resistivity structure in this district was quite low in contrast or 1 ohm/m against 30 ohm/m, but the result of 3-D model calculation indicated that a sufficient analysis would be possible even in such a low contrast case. Furthermore, as the result of the study of the density of observation stations in the magnetic field, it was found that changes in the response of magnetic field to the resistivity structure would be approximately 2% at the maximum and that the ill effect on the result of measurement would be of the negligible magnitude even when a single magnetic field observation station is deployed against plural electric field observation stations. 6 refs., 7 figs.

  3. 3D seismic experiment in the Minaminoshiro area, Akita. Data processing; Akitaken Minaminoshiro chiiki ni okeru sanjigen jishin tansa jikken. Data shori

    Energy Technology Data Exchange (ETDEWEB)

    Tanaka, H; Minegishi, M [Japan National Oil Corp., Tokyo (Japan). Technology Research Center; Nakagami, K [Japan Petroleum Exploration Co. Ltd., Tokyo (Japan)

    1997-10-22

    A 3D seismic experiment was carried out in the Minaminoshiro area in Akita Prefecture, an area difficult of performing seismic exploration. This paper reports progresses during data processing and future problems. The data processing has executed static correction of 3D refraction, 3D DMO correction, and an F-X prediction filter processing on the data in time domain in the 3D seismic exploration as acquired in a spread of 4 km times 5 km in the subject area. The result of the data processing verified existence of a folding structure and the Noshiro thrust fault groups in the east to west direction, and locations of the Sakagawa fault associated therewith. Seen particularly noticeably was a structure having a slope falling north-ward at 15 to 35 degrees in shallow and deep portions on the east side of the Sakagawa fault in the south to north direction. In addition, the Dogiri fault was identified, which has been though to exist in a direction crossing perpendicularly the Noshiro thrust fault groups. It is scheduled that spatial velocity will be analyzed, and data processing will be conducted for deep regions. 7 figs.

  4. High resolution aeromagnetic and radiometric survey in the Hokusatsu district, Japan; Hokusatsu Kushikino chiiki ni okeru kobunkaino kuchu jiki hoshano tansa kekka ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    Hishida, H; Yoshioka, K [Metal Mining Agency of Japan, Tokyo (Japan)

    1997-10-22

    In FY 1996, aeromagnetic and radiometric survey was performed in the whole area of Hokusatsu district using a helicopter fixed with a stinger mount system with high density 200 m line spacing at low altitude of 80 m, which was the first in Japan. Based on the existing geological maps, Landsat TM data and radiometric data, lithologies and geological structures were extracted in response to individual magnetic characteristics in the whole magnetic map. Obtained geological characteristics are as follows. Straight magnetic anomalies suggested volcanic lava flows. Isolated ring-shaped magnetic anomalies suggested intrusive rocks. Ring-shaped weak magnetic anomalies suggested the centers of volcanoes. Wide range intense magnetic anomalies were caused by deep intrusive rocks. Wide range weak magnetic anomalies were caused by dissipation of magnetite due to hydrothermal alteration. Faults and fracture zones were recognized as linear configurations of magnetic anomaly and divided magnetic anomalies. Based on these results, local geological structures, hydrothermal alterations, and mineralization of known ore deposits were interpreted. 6 refs., 5 figs.

  5. Wave fields simulation in difficult terrain using numerical grid method; Hyoko henka no aru chiiki deno suchi koshi wo mochiita hado simulation

    Energy Technology Data Exchange (ETDEWEB)

    Jung, W; Ogawa, T [Yokohama National University, Yokohama (Japan); Tamagawa, T; Matsuoka, T [Japan Petroleum Exploration Corp., Tokyo (Japan)

    1997-10-22

    This paper describes that a high-accuracy simulation can be made on seismic exploration by using the numerical grid method. When applying a wave field simulation using the difference calculus to an area subjected to seismic exploration, a problem occurs as to how a boundary of the velocity structure including the ground surface should be dealt with. Simply applying grids to a boundary changing continuously makes accuracy of the simulation worse. The difference calculus using a numerical grid is a method to solve the problem by imaging a certain region into a rectangular region through use of variable conversion, which can impose the boundary condition more accurately. The wave field simulation was carried out on a simple two-layer inclined structure and a two-layer waved structure. It was revealed that amplitudes of direct waves and reflection waves are disturbed in the case where no numerical grid method is applied, and the amplitudes are more disperse in the reflection waves than those obtained by using the numerical grid method. 7 refs., 10 figs.

  6. Fiscal 1999 technical survey report. Basic survey of prioritized fields in South Asia (India); 1999 nendo Minami Asia chiiki (Indo) juten bun'ya kiso chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    For working out and proposing project themes optimum for the popularization of technologies for effectively utilizing energy in India, a basic survey was conducted into India's energy policy, trend of supply and demand, steelmaking industry, papermaking industry, petrochemical industry, and chemical fertilizer making industry. India in fiscal 1997 consumed 374-million tons of energy in terms of oil, 30% in oil and 63% in coal. Activities were vigorous for the development of oil substitutes such as photovoltaic and wind power. The steelmaking industry that produced 22.12-million tons in fiscal 1996 was found to suffer from a reduced operating rate due to aging of facilities and from poor infrastructures such as harbor and railway facilities wanting maintenance. It was inferred that energy conservation facilities and technologies of Japan's steelmaking industry would work effectively. The cement making industry was found modernized, but faced the problem of the high cost of electric power. The papermaking industry was busy enhancing its own production capability. The fertilizer making industry ranked third in the world in terms of production, but the energy consumption rate at its ammonia plant was too high. The petrochemical industry was well developed, with Japan's technologies incorporated into it in the design stage. (NEDO)

  7. Report on feasibility study of district energy-saving and waste heat utilization for City of Iwai; Iwai-shi chiiki sho energy hainetsu riyo kanosei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    As part of the (district energy-saving visions for City of Iwai), the feasibility study is implemented for citizen community facilities which utilize waste heat discharged from factories in the city. More concretely, those items studied include a heated pool, other community facilities and botanical garden of tropical plants which utilize waste heat of exhaust gas cooling water heated to around 70 degrees C by a desulfurization unit at a pulp factory. Case 1 includes the citizen community facilities (e.g., heated pool and bath facilities), and Case 2 includes a green house botanical garden, involving studies on facility scales, requirements of heat and recycling water, hot water supply, air conditioning, bath systems, and heating systems for green houses. It is estimated that the citizen community facilities have an energy saving effect of 640kL/y as fuel oil, which corresponds to saving of around 29 million yen/y, and CO2 abatement effect of 471t/y as carbon, and that the green house botanical garden has an energy saving effect of 669kL/y as fuel oil, which corresponds to saving of around 30 million yen/y, and CO2 abatement effect of 492t/y as carbon. (NEDO)

  8. Regional new energy vision formulation project for Yobuko Town. Fiscal 2001 report; 2001 nendo Yobuko cho chiiki shin energy vision sakutei jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for elevating people's consciousness of such at Yobuko Town, Saga Prefecture, surveys and studies were conducted involving the amount of energy needed by the town, the amount of new energy resources in existence, and new energy introduction programs, and a vision was formulated. The demand for energy of the town is estimated at 105,040 drums/year in terms of crude oil - 37% in electric power, 34% in fuel oil A, 13% in gas oil, and 8.1% in gasoline. As for the presence of new energy resources, it is deemed that wind and solar energies will be richly available. Studies were conducted for new energy introduction programs, which included the introduction of photovoltaic power into a nursing home for aged people, primary and junior high schools, and a water reservoir; street lamps lighted by a hybrid power system and a solar cell clock for the plaza in front of the harbor; introduction of wind power into the little islands of Ogawa and Kabe; installation of a solar energy collector for the nursing home for aged people; and the use of clean energy vehicles for official business. It is estimated that the efforts will bring about a 1,256.6 t-C reduction in carbon dioxide emissions. (NEDO)

  9. Report for fiscal 1979 on survey for development of overseas coals. Blau area, Kalimantan, Republic of Indonesia; 1979 nendo kaigaitan kaihatsu chosa hokokusho. Indonesia kyowakoku Kalimantan Blau chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1979-12-01

    Surveys including collection of information and materials relative to various conditions were performed on Republic of Indonesia, who is considered a promising coal supplying source for Japan in the future. Based on the assumption that supply capacity of coal will be expanded for thermal power plants, and large rise is anticipated in the coal price, and in order to solve the problems in the plan for migrating the population from Jawa Island where serious population concentration has been taking place, and in the regional promotion and development, the Indonesian Government has made open to foreign business entities in June 1978 the development of coal in the Kalimantan area. Nine corporations including those from Japan have participated in the international bid. The Indonesian Governments seeks in this project a product sharing system, rather than the acquisition of foreign currencies or the profit sharing from export earnings. The Government considers Japan as one of the coal exporting markets, and expects utilization of the Japan's advantageous institutional financing. It is urged that Japan should discuss the coal development in the Kalimantan area as part of dispersion of the coal supply sources upon considering the ocean transportation distance and the close economic relationship with the country. (NEDO)

  10. Report on Symposium on Pacific Energy Cooperation 2001. Energy Security in Asian Region; Taiheiyo energy kyoryoku kaigi 2001 hokokusho. Asia chiiki no energy anzen hosho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-20

    The Symposium on Pacific Energy Cooperation (SPEC), which started in 1986 to discuss energy problems and international cooperation on energy in the Pacific region, met for 2001 in Tokyo on February 20 and 21, 2001, to discuss Energy Security in Asian Region. The conference comprised two keynote addresses, four sessions, and a panel discussion. Twenty-four countries and an international organization were represented by a total of 785 participants who actively exchanged views and opinions. With Director Tsutomu Toichi, The Institute of Energy Economics, Japan, acting as chairman, two keynote addresses were delivered. Minister of Oil Bijan Namdar Zanganeh of Iran reported on The Role of I. R. Iran in Meeting the Growing Oil and Gas Demand in the Asia-Pacific Region and Potential Cooperation with the Region's Consumers. Managing Director Phil Watts of the Royal Dutch/Shell Group reported on Security in Trust - Meeting Asian Energy Needs in the 21st Century. (NEDO)

  11. Report on the geothermal development promotion survey. No.38. Mt. Aso West area; Chinetsu kaihatsu sokushin chosa hokokusho. No. 38 Asozan seibu chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-03-01

    The paper summed up the results of the geothermal development promotion survey 'Mt. Aso West area' which was carried out at Choyo village and Kugino village, Aso county, and at Otsu town, Kikuchi county, Kumamoto prefecture, from FY 1991 to FY 1994. In the survey, the following were conducted for the comprehensive analysis: surface survey such as geology/alteration zone survey, geochemical survey and high precision MT method survey, and temperature log, electrical log, water injection test, core test and hydrothermal survey by drilling 7 structural boreholes. In the numerical analysis of thermal structure models, the present geothermal manifestation was obtained, assuming that the past magma reservoir in a state of agglomeration/semi-agglomeration exists in the depths of caldera and that the depth is set at 4km. It is assumed that the deep hydrothermal reservoir around Yunoya/Tarutama exists around the depth of 500-600m below sea level and that the temperature is 230-300 degrees C. The meteoric water permeating into the depths forms high-temperature reservoirs around Yunoya/Tarutama. The reservoir rises up to about 400m below sea level to become a vapor-dominated phase. It forms an acidic SO{sub 4} type reservoir phase around the earth surface. (NEDO)

  12. High-resolution aeromagnetic survey in the area of Arima-Takatsuki-Rokko active fault system; Arima Takatsuki Rokko katsudanso chiiki ni okeru komitsudo kuchu jiki tansa

    Energy Technology Data Exchange (ETDEWEB)

    Nakatsuka, T; Okuma, S; Morijiri, R; Makino, M [Geological Survey of Japan, Tsukuba (Japan)

    1996-10-01

    High-resolution aeromagnetic survey was conducted in December, 1995, over the area of Arima-Takatsuki-Rokko active fault system, where the 1995 Hyogo-South (Kobe) earthquake took place. Based on the surveyed data, the magnetic anomaly distribution in the Kei-Hanshin district was illustrated. Features of magnetic anomaly observed in it were discussed. Influence of artificial structures in the coastal zone of Kobe City was tried to be removed. Distinct magnetic anomaly observed in the center of Kyoto City was located at the southern part near Shijo-Karasuma. This was mainly affected by the artificial structure. A high magnetic anomaly with a long wave length was observed in the southern part of Kyoto. There was a high magnetic anomaly in an area surrounded by central cities of Nishinomiya-Toyonaka district. At the location where positive and negative magnetic anomalies were illustrated in NEDO`s map in the coastal zone of Kobe City, the anomaly pattern was clarified. The influence of artificial structure was approximated using a point dipole. The dipole was placed in each block having a magnetic anomaly. The parameter was determined by the inverse analysis, to remove the influence. Consequently, most of magnetic anomalies due to artificial structures could be removed. 6 figs.

  13. Change in surface SP caused by pressure buildup observed at the Nigorikawa geothermal area; Nigorikawa chiiki ni okeru atsuryoku buildup ji no shizen den`i henka

    Energy Technology Data Exchange (ETDEWEB)

    Yasukawa, K; Yano, Y; Matsushima, N; Ishido, T [Geological Survey of Japan, Tsukuba (Japan); Takahashi, M; Suzuki, I; Aoyama, K; Kuwano, T

    1996-10-01

    To examine the effect of change of subsurface flow system on the surface SP (self potential), SP measurements were carried out before and after the pressure buildup and drawdown during the periodic inspection at Nigorikawa area. Relation between the SP distribution and the observed data was also examined by 2-D numerical simulation. Tendency was found that the SP increased gradually with the production near the production well, decreased during the pressure buildup, and increased again during the drawdown. There were some points having the reverse tendency in the surrounding area. Behavior during the pressure buildup and drawdown was not clear. The resistivity near the ground surface was low ranging between 2 and 5 ohm/m within the Nigorikawa basin. The variation of SP was not so large when compared with the measuring error. The SP profiles on the secondary section passing in the center of caldera at the production stop and at one week after the production start were well corresponded with the profiles under natural conditions which were reproduces using the 2-D model. It was considered that the SP profile before the production stop was affected by the production. 12 refs., 6 figs., 1 tab.

  14. Geoelectrical structure by electrical logs and Schlumberger sounding at the Akinomiya geothermal field, Akita Prefecture; Denki kenso oyobi Schlumberger ho ni yoru Akinomiya chinetsu chiiki no hiteiko kozo

    Energy Technology Data Exchange (ETDEWEB)

    Kajiwara, T; Takemoto, S

    1997-05-27

    Based on the electrical logging data of the existed well and Schlumberger sounding data obtained in 1974, a two-dimensional inversion analysis of the specific resistance profile was conducted at the Akinomiya geothermal field, Akita Prefecture. From the electrical logging data, relationships between the geology and the specific resistance were illustrated. The specific resistance values of basement rocks showed more than 100 ohm-m, which were higher than those of the other seams. Intrusive rocks and tuffs in the basement rocks showed locally low values less than 100 ohm-m. Younger volcanic rocks showed low values around 10 ohm-m. As a result of the two-dimensional inversion analysis, the basement rocks could be detected as high specific resistance layers. Accordingly, it was considered that the basement rocks in this field can be detected as high specific resistance layers by analyzing the results of field survey sufficiently. Low specific resistance zones were observed in the shallow depth, which corresponded to the fumarolic gases. There were some layers with remarkably varied specific resistance values, which were considered to be related with alteration. 6 refs., 3 figs.

  15. Subsurface interpretation based on geophysical data set using geothermal database system `GEOBASE`. 2; Chinetsu database system `GEOBASE` wo riyoshita Kakkonda chinetsu chiiki no chika kozo kaiseki. 2

    Energy Technology Data Exchange (ETDEWEB)

    Osato, K; Sato, T; Miura, Y; Yamane, K [GERD Geothermal Energy Research and Development Co. Ltd., Tokyo (Japan); Doi, N [Japan Metals and Chemicals Co. Ltd., Tokyo (Japan); Uchida, T [New Energy and Industrial Technology Development Organization, Tokyo, (Japan)

    1997-05-27

    Five cross sections were applied as a result of MT method investigations in addition to the results of conventional analyses at the Kakkonda geothermal area; three-dimensional resistivity distribution was made into a database by using the Kriging method which makes a matching with anisotropy of seismic center distribution in micro-earthquakes; and the database was compared with the data derived from surveys on the pilot survey well WD-1a and the side-truck well WD-1b thereof. As a result, it was found that the well WD 1b which encountered a water loss zone had the water loss zone exist in a region with relatively lower resistivity than in the well WD-1a which did not encounter a water loss zone. The region in which the water loss zone was encountered existed in a very steep slope region going from the high resistivity region in the west side toward the low resistivity region in the east side. This fact suggests a possibility that fractures have developed in this region with sharp slope in the resistivity in this area. Adding three-dimensional complementary function to the GEOBASE database by using a simple Kriging allowed the direction of anisotropy in spatial data to be freely and quickly decided. It was learned that this capability exhibits strong power in a mapping work in structures where such anisotropy as a geothermal zone is highly dominant. 5 refs., 8 figs., 2 tabs.

  16. Geothermal development promotion survey report. No. 25. Hishikari region; 1987-1989 chinetsu kaihatsu sokushin chosa hokokusho. No. 22 Hishikari chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1990-03-01

    The results of surveys conducted in the Hishikari region, Kagoshima Prefecture, in fiscal 1987-1989 are compiled in this report. Conducted were a geological/alteration zone survey, geochemical survey, electric prospecting, electromagnetic surveillance, gravity prospecting, heat flow rate survey, test boring, environmental impact survey, and so forth. The surveys resulted in conclusions mentioned below. According to the underground temperature distribution based on the results of the heat flow rate survey, test boring, and so forth, temperature is low at the western part of the Hishikari region where there is a low gravity anomaly and high in the zone in the ENE-WSW direction where there is a high gravity anomaly. The present ground temperature is lower than the fluid inclusion homogenization temperature by approximately 120-140 degrees C. It is deduced that the geothermal water reservoir lies in the Quatenary volcanic rocks or in a fracture zone that develops in the Shimanto supergroup. It is inferred that the geothermal water producing the hot spring water all originates in meteoric water staying long in the ground. It is also inferred that volcanic gas or the like contributes but a little to the formation of the geothermal system but that the contribution is great of the heat supplied from the magma pool. (NEDO)

  17. Regional new energy vision formulation project for Ukiha Town. Fiscal 2001 report (Summary); 2001 nendo Ukiha machi chiiki shin energy vision sakutei tou jigyo hokokusho (Gaiyo ban)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For contributing to the preservation of the global environment through new energy application and energy conservation, efforts were made to work out a new energy vision for Ukiha Town, Fukuoka Prefecture. Town's population was 17,482 in fiscal 2000, which showed a tendency to decline slowly. The town demands 158,886 drums/year in terms of crude oil - 56.2% in electric power, 19.5% in gasoline, and 10.5% in gas oil. As for the presence of new energy resources, wind power comes in first in terms of magnitude, and woody biomass energy is also found in abundance since lumbering prospers in the town. Named as candidate projects in the efforts to introduce new energies were the embodiment of practical size wind power systems in the hilly part; heat utilization and power generation using biomass such as scrap wood from lumber mills; introduction of photovoltaic power into public facilities; use of clean energy vehicles by the town office; photovoltaic power generation and solar heat utilization at welfare facilities and public gymnastic halls; street lamps lighted by hybrid power systems in the shopping area and at the Michinoeki roadside service spot; use of new energies in agriculture; financial aids to encourage photovoltaic power into general residences; etc. (NEDO)

  18. FY 2001 new energy vision of Shiramine Village. Creation of new energy; 2001 nendo Shiramine mura chiiki shin energy vision. Onkotakushin energy no sozo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Shiramine Village, Ishikawa Prefecture, an investigational study was conducted of the energy demand amount of the village, existence amount of new energy and project for new energy introduction, and a vision was worked out. The energy demand amount of the village was 155,970 GJ/y. As viewed by sector, the energy demand amount consisted of 43.9% in the industrial sector, 30.0% in the commercial/residential sector and 26.1% in the transportation sector. The rate of energy source was 79.0% of petroleum, 18.7% of electric power and 2.2% of gas. In the project for new energy introduction, the following were cited and studied: as the project for forest/snow resource utilization in the Nishiyama area, production/storage of charcoal using thinnings/wood chips, and food preservation using snow; as the project for experiencing/learning the nature using existing facilities, introduction of photovoltaic power system/solar heat use water warmer to Shiramine Elementary School/Shiramine Junior High School; as the project for functionally reinforcing tourist attractions, 'park for learning new energy' by lighting using solar light/wind power, etc. (NEDO)

  19. Report on the survey for working out the new energy vision of the Yamagata city area; 2001 nendo Yamagata machi chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Yamagata City, Ibaraki Prefecture, an investigational study was conducted of the energy consumption amount of the city, existence amount of new energy and project for new energy introduction, and a vision was worked out. The energy consumption of the city in FY 1999 was 178.8 billion kcal in amount and was broken down into 76.5% of petroleum, 18.8% of electric power and 0.85% of LPG. As viewed by sector, the energy consumption consisted of 37.9% in the transportation sector, 35.2% in the industrial sector and 26.8% in the commercial/residential sector. As the project for new energy introduction, the following were studied: biomass cogeneration/greenhouse using waste heat from hot spring/photovoltaic power generation/wind power - photovoltaic power hybrid street light/biomass vehicle in the Santanoyu/Eco-Zone formation project; introduction of photovoltaic power generation/solar heat utilization to gymnasium of junior high school/multi-purpose cultural center/municipal dwelling house/elementary school/junior high school/central public hall in the new energy project for public facilities; introduction of clean energy vehicle, etc. (NEDO)

  20. Fiscal 1999 technical survey report. Basic survey of prioritized fields in East Asia (China); 1999 nendo Higashi Asia chiiki (Chugoku) juten bun'ya kiso chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    For working out and proposing project themes optimum for the popularization of technologies for effectively utilizing energy in China, a basic survey was conducted on China's energy policy, trend of supply and demand, cement making industry, non-ferrous metal industry, chemical industry, and electric power industry. The amount of primary energy produced in China in fiscal 1997 was 1.4-billion tons in terms of standard coal, ranking third in the world. It produced 1.4-billion tons of coke making coal, 160-million tons of oil, and 110-million kWh of electric power. In spite of its continuous efforts to develop oil and in particular natural gas, it has now turned an oil importing country. There was an increase in production in the industries surveyed, but energy efficiency was low because of their small-scale old-fashioned processes. In the cement making industry, 86% of the facilities presented some problems, and the non-ferrous industry was planning to rebuild itself into large-scale advanced-process plants. As for the chemical industry, investigations were conducted into the oil refining, petrochemical, and chemical fertilizer making industries. The electric power industry had facilities for 277GW, 210GW in thermoelectric power generation and 65GW in hydroelectric power generation. The thermoelectric power plants were found to emit great quantities of smoke and sulfuric oxides into the air. (NEDO)

  1. Fiscal 1999 geothermal energy development promotion survey. Report on resource assessment for Shiramizugoe area; 1999 nendo chinetsu kaihatsu sokushin chosa hokokusho. Shiramizugoe chiiki shigen hyoka

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    Under an assumed plan of building a geothermal power station in the Shiramizugoe area of Makizono-cho, Aira-gun, Kagoshima Prefecture, investigations will be conducted under a 4-year program into the amount of geothermal resources, the dimensions and cost performance of a geothermal power station under consideration, and the environmental impact that the locating of such will incur, through for example identifying areas containing geothermal reservoirs suitable for geothermal power generation. In fiscal 1999, activities were conducted in the three fields of (1) survey plan preparation, (2) comprehensive analysis, and (3) environmental assessment. Under item (1), a survey and coordination implementation plan was prepared, existing data were analyzed for the construction of geothermal models out of which a suitable geothermal structure was specified for digging, and a list was drafted of locations for borehole digging for fiscal 2000. Under item (2), it was found that most of the past surveys covered the Ogiri area and that the Shiramizugoe area was but poorly covered. Discussion was made on the geological structure, geothermal structure, geothermal water hydraulic structure, and the geothermal water system. Under item (3), the plan was summarily explained to the local administration, associations of hot spring hotels, and inhabitants in the vicinity, and their consent was obtained. (NEDO)

  2. Report on the survey for working out the new energy vision of the Shiga village area; 2001 nendo Shiga mura chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it at Shiga Village, Nagano Prefecture, an investigational study was conducted of the energy demand in the village, existence amount of new energy, project for new energy introduction, etc., and a vision was worked out. The energy consumption amount in FY 2000 in Shiga Village was approximately 50.2 billion kcal and was broken down into 54.7% of petroleum, 38.3% of electric power, 6.3% of LPG and 0.75% of others. As the project for new energy introduction, the following were studied: installation of photovoltaic power generation panels to the new office under construction, introduction of photovoltaic power/wind power hybrid power generation to Klein Garten Club House, installation of the solar system to hot spring facilities in the village, project on low-temperature wood drying by smoking using sawdust/wood chips, methanol production using thinnings as raw materials and the use for automobile fuel/cogeneration, methane fermentation from animal feces/house garbage and the use for cogeneration, etc. (NEDO)

  3. Report on a feasibility study of making an international joint research on energy in the Asia region; Asia chiiki kokusai kyodo kenkyu kanosei chosa hokokusho. 2

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    A survey was conducted on the energy situation in Thailand, Indonesia, Malaysia, the Philippines and India, and a possibility of making an international joint research. Thailand has sharply increased investments from Europe and the U.S. since 1985, and has been making a rapid industrialization. As to the energy policy, it is important to secure the stable supply and stabilize the price. Indonesia has been rapidly increasing energy consumption in accordance with the recent economic growth, and is fear of becoming a net oil importing country in the near future. They are taking a policy for securing as much domestic oil and gas as possible. Malaysia has been keeping a high growth of over 8% per year for the past 8 years and rapidly increased energy demand. It is expected that they will maintain a high level of the development of energy resources. In the Philippines, the industrial energy is mostly coal, but electricity expected in future is from the oil-fueled power generation. The stable import of oil is a problem. 45 figs., 51 tabs.

  4. Report on the geothermal development promotion survey. No.38. Mt. Aso West area; Chinetsu kaihatsu sokushin chosa hokokusho. No. 38 Asozan seibu chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-03-01

    The paper summed up the results of the geothermal development promotion survey 'Mt. Aso West area' which was carried out at Choyo village and Kugino village, Aso county, and at Otsu town, Kikuchi county, Kumamoto prefecture, from FY 1991 to FY 1994. In the survey, the following were conducted for the comprehensive analysis: surface survey such as geology/alteration zone survey, geochemical survey and high precision MT method survey, and temperature log, electrical log, water injection test, core test and hydrothermal survey by drilling 7 structural boreholes. In the numerical analysis of thermal structure models, the present geothermal manifestation was obtained, assuming that the past magma reservoir in a state of agglomeration/semi-agglomeration exists in the depths of caldera and that the depth is set at 4km. It is assumed that the deep hydrothermal reservoir around Yunoya/Tarutama exists around the depth of 500-600m below sea level and that the temperature is 230-300 degrees C. The meteoric water permeating into the depths forms high-temperature reservoirs around Yunoya/Tarutama. The reservoir rises up to about 400m below sea level to become a vapor-dominated phase. It forms an acidic SO{sub 4} type reservoir phase around the earth surface. (NEDO)

  5. Fiscal 2000 survey report on regional new energy vision formulation for Oki town; 2000 nendo Okicho chiiki shin energy vision sakutei nado chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    Efforts are in progress to build a resource circulation type town symbiotic with natural environments at Oki town, Fukuoka Prefecture, which is an agricultural community where creeks run in every direction. A new energy vision has been worked out for the town, which centers about a plan for building a resource circulation type community. The survey results are summarized in six chapters dealing with (1) the survey outline, (2) local conditions relative to energy, (3) energy supply/demand structure, (4) amount of new energy in existence, (5) records and analysis of inhabitants' approach to energy conservation, and (6) a new energy introduction plan. The new energy introduction plan consists of four items involving a short term plan or a key project, a project for medium/long term surveys or feasibility study for the commercialization of new energy, plans for reducing energy consumption, and so forth. Discussed in relation to the short term plan are the introduction of photovoltaic power generation into public facilities, installation of a photovoltaic power station to be jointly utilized by the inhabitants, establishment of a grant system for the installation of solar water heaters for domestic use, creation of an additional subsidy system for domestic photovoltaic power generation, and the like. (NEDO)

  6. Fiscal 2000 report on regional new energy vision formulation project for Yunomae town, Kumamoto prefecture; 2000 nendo Yunomaecho chiiki shin energy vision sakutei nado jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A regional new energy vision has been formulated for Yunomae town, Kumamoto Prefecture, from the standpoint that local autonomies are required to work out measures compatible with Government energy policies and thereby to wrestle with environmental measures being undertaken across the world. The results of activities conducted for this purposed are stated in six chapters, which cover (1) the meaning of regional new energy vision formulation and its position, (2) outline of Yunomae town, (3) energy and environments, (4) energy situation in Yunomae town, (5) Yunomae town's regional new energy vision, and (6) plans for promoting the embodiment of the vision. In chapter (5), basic policies toward new energy utilization, overall plan, leading projects, and energy conservation measures are stated. The types of new energy taken up for discussion are photovoltaic power, wind power, biomass power, refuse-derived energy, and hydraulic energy. Projects are subjected to study, which relate to photovoltaic power generation, solar heat utilization, small scale hydroelectric power, small scale wind power, and the introduction of clean energy vehicles. It is expected that these projects when carried out will cover approximately 10% of the current energy consumption of the town. (NEDO)

  7. Survey of regional new energy vision formulation for Shimizu town. Report on initial stage survey; Shimizucho chiiki shin energy vision sakutei chosa. Shoki dankai chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    Shimizu town, Hokkaido, is a 11,000-strong town, located in the northwestern part of the Tokachi plains and consuming 1.7 times 10{sup 4} Gcal in energy. Discussion is held for new energy which will make the most of the local characteristics of the town while the basics of town building are considered and confirmed. The results of the survey are summarized in four chapters which are (1) the purpose of regional energy vision formulation, (2) local characteristics, (3) state of new energy in existence, (4) and the course to follow for the introduction of new energy (draft). Chapter (4) is constituted of the purpose of new energy introduction and the basic policy, and the course along which new energy will be adopted. Proposed in relation with the course of new energy introduction are the effective utilization of biomass resources, utilization of cold heat energy (ice) involving agriculture, introduction of a symbolic small scale water energy system, introduction of a symbolic wind energy system, extensive introduction of solar energy, and the deployment of popularizing and enlightening activities. A project is mentioned of experimentally introducing biogas facilities that makes use of farm animals' excretion. (NEDO)

  8. Report on the geothermal development promotion survey. No.36. Mt. Amemasudake area; Chinetsu kaihatsu sokushin chosa hokokusho. No. 36 Amemasudake chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-03-01

    The paper summed up the results of the geothermal development promotion survey 'Mt. Amemasudake area' which was carried out at Akaigawa village, Yoichi county, Hokkaido, from FY 1991 to FY 1994. In the survey, the following were conducted for the comprehensive analysis: surface survey such as geology/alteration zone survey, geochemical survey, gravity exploration, electromagnetic exploration and electric exploration, core test by drilling 5 boreholes, test to induce jetting of geothermal fluids, measurement of in-borehole temperature/pressure, survey of geochemical properties of geothermal water. As to the fracture system in this area, the Amemasudake fault and the Amemasuzawa fault are especially important, and it was assumed that these faults had relation to the present geothermal distribution. It is thought that structural conditions of geothermal reservoirs are fractures in basement rocks. As a result of the borehole survey, it was indicated that the center of the high-temperature part with a temperature of 250 degrees C or more was in the boundary zone southeast of this area at a level of 500m below sea level. The scale, which reached about 3km both in east/west and north/south, is almost the same scale as that of the neighboring Toyoha area where great potentiality of the geothermal development is expected. (NEDO)

  9. Report on the geothermal development promotion survey. No.C-2. Wasabizawa area; Chinetsu kaihatsu sokushin chosa hokokusho. No.C-2 Wasabizawa chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    The paper summed up the results of the survey of the geothermal development promotion survey - Wasabizawa area which was carried out in Yuzawa City and Ogachi Town, Akita Prefecture, from FY 1993 to FY 1996. In the survey, the following were conducted: surface survey such as geological alteration zone survey, fluid geochemical survey, gravity survey and electromagnetic exploration, temperature log by drilling 9 exploration wells, short-term/long-term jetting test, etc. Further, using the data obtained from these, analysis was made of geology/reservoir structure, thermal structure, geothermal water/hydraulic structure, geothermal system models, etc. As a result, a high-temperature (about 300 degrees C) zone promising as the geothermal development area was picked out, and the existence was confirmed of a dominant geothermal reservoir around the fault. In the jetting test, a total steam amount of 111.6 t/h was confirmed, and from the reservoir simulation, the result was obtained that there was a high possibility of 30-year power generation of 30MW/y. As a result of studying 'a power generation system by small-scale unit serial development' considering regional characteristics in the Wasabizawa area, the power generation cost (sending end, 15-year average) was estimated at approximately 9-10 yen/kWh. (NEDO)

  10. Geothermal development promotion survey report. No. 22. Noboribetsu region; 1987-1990 chinetsu kaihatsu sokushin chosa hokokusho. No. 22 Noboribetsu chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1991-03-01

    The results of surveys conducted in the Noboribetsu region, Hokkaido, in fiscal 1987-1989 are compiled in this report. Conducted in the surveys were a geological/alteration zone survey, geochemical survey, electromagnetic surveillance (simplified magnetotelluric method), electric prospecting (Schlumberger method), electric prospecting (mise-a-la-masse method), heat flow rate survey, structural boring, precision structural boring, environmental exploration well, geothermal water survey, environmental impact survey, and so forth. Conclusions reached on the basis of the survey results are described below. It is supposed that a horizon, positioned in the Osarugawa stratum in the Karls Noboribetsu zone or in a fissure system in the Omagarisawa stratum below the Osarugawa stratum, contains a geothermal reservoir. The hot water at the Noboribetsu hot spring originates in gas or geothermal water separated from the deep-seated geothermal water while that at the Karls hot spring or the like originates in meteoric water built up in higher places. Although an area abundant in geothermal fluids is supposed to exist in the Karls-Noboribetsu zone, yet a section located between the Karls-Noboribetsu zone and the Noboribetsu hot spring area also draws attention as a zone having a potential to store geothermal fluids. (NEDO)

  11. Geothermal development promotion survey report. No. 28. Eastern part of Obanazawa region; 1988-1990 chinetsu kaihatsu sokushin chosa hokokusho. No. 28 Obanazawa tobu chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1991-03-01

    The results of surveys conducted in the eastern part of Obanazawa region, Yamagata Prefecture, in fiscal 1988-1989 are compiled in this report. Conducted were a geological/alteration zone survey, geochemical survey, electromagnetic surveillance (TDEM - time domain electromagnetic method), gravity prospecting (review of gravity data), electric prospecting (Schlumberger method), heat flow rate survey, test boring, environmental impact survey, and so forth. Conclusions are mentioned below. It is inferred that the water of the Ginzan hot spring of the neutral Cl-SO{sub 4} type originates in meteoric water in mountains high above the mean sea level in the western side and that the hot spring water is produced when water heated to approximately 170 degrees C at a depth (1,500-2,000 meters below mean sea level) in the Ginzan hot spring district, where the ground temperature is the highest in this region, is diluted by groundwater near the surface at its ultimate stage of ascent. The survey results disclose that possibilities are quite low that a high-temperature sector of 200 degrees C or higher is found at a level not deeper than 2,000 meters from the ground surface. Accordingly, no geothermal development by flash steam power generation is feasible at the present stage at any economically acceptable depth. (NEDO)

  12. Report on the geothermal development promotion survey. No.C-3. Akinomiya area; Chinetsu kaihatsu sokushin chosa hokokusho. No.C-3 Akinomiya chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The paper summed up the results of the survey of the geothermal development promotion survey - Akinomiya area which was carried out in Yuzawa City and Ogachi Town, Akita Prefecture, from FY 1996 to FY 1999. In the survey, the following were conducted: surface survey such as the geological alteration zone survey, fluid geochemical survey, gravity survey and electromagnetic exploration, logging by drilling 8 boreholes, jetting test, etc. Further, using the data obtained from these, analysis was made of geology/reservoir structure, thermal structure, hydraulic structure, etc. As a result, grasped were the distribution of the main underground fracture structure and the distribution of promising high-temperature zones. In the short-term/long-term jetting test, a total steam amount of 124 t/h was confirmed. Moreover, there was the pressure interference between the geothermal reservoir in the Akinomiya area and the geothermal reservoir in the Wasabizawa area, and it was confirmed that there was the hydraulic relation between those reservoirs. Further, it was assumed that there was not so much effect on hot spring reservoirs in the Akinomiya area. In the simulation of production prediction, the result was obtained that there was a possibility of power generation of 30MW only in the Akinomiya area and of 60MW both in the Akinomiya area and the Wasabizawa area. (NEDO)

  13. Fiscal 2000 basic survey report for vision formulation. Regional new energy vision for Sabae city, Fukui prefecture; Sabaeshi chiiki shin energy vision. 2000 nendo sakutei kiso chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Sabae city, Fukui Prefecture, has worked out a regional new energy vision for which the inhabitants, industrialists, and administrators combined their efforts to build a daily life related culture, friendly to environments and peculiar to the locality. The fruits of their activities are summarized in five chapters, which are (1) the basic philosophy, (2) survey of the amounts of new energy in existence, (3) survey of the amount of energy demanded, (4) various surveys conducted with the participation of the citizenry, (5) and summarization of important topics. Discussed in chapter (2) is the basic policy toward reckoning the amounts of new energy in existence, such as photovoltaic energy, solar heat energy, wind energy, hydraulic energy, temperature difference energy, and other energy resources remaining to be utilized. It states that they, when converted into electric power, 2.0 times 10{sup 8} kWh/year will be available and, when converted into heat energy, 2.92 times 10{sup 11} kcal/year will be available, accounting for approximately 30% of the city's energy consumption. When the energy resources are broken down by type, it is found that solar energy, wind energy, and refuse-derived energy are in existence aplenty. (NEDO)

  14. FY 2000 report on the analytical survey of the basic data by area; 2000 nendo chosa hokoku. Chiiki betsu kiso data bunseki chosa

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    From a viewpoint of the spread of energy conservation technology, etc. that Japan owns, following the previous fiscal year, data collection/study were made of 51 countries and 1 area including 49 countries (17 Asian developing countries, 13 Middle East countries and 19 countries in East Europe/Former U.S.S.R.) and 2 countries (Japan/the U.S.) and 1 area (the EU) that were added for comparison with developed countries. Items for study were the general situation, economic/trade indicators, administration/general situation, energy situation, economic/technical cooperation from Japan, implementing situation of NEDO's model project, AIJ implementing situation in each country, etc. To keep the continuity, data were numerically obtained from the same data source as that used in the previous fiscal year as a rule. The input data were made as consistent in each item as possible, and the same table was obtained from a single data source as a rule. The data in the previous fiscal year were reviewed, and the data were adopted from which data on as many countries as possible could be acquired through the same data source. (NEDO)

  15. Fiscal 1999 technical survey report. Basic survey of prioritized fields in Central Asia; 1999 nendo Chuo Asia chiiki juten bun'ya kiso chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    For working out and proposing project themes optimum for the popularization of technologies for effectively utilizing energy in four countries in Central Asia (Kazakhstan, Uzbekistan, Turkmenistan, and Azerbaijan), basic surveys were conducted into their energy policies, trends of supply and demand, electric power industry, metal refining industry, and cement making industry. Common to the four nations were that they were rich in energy resources (natural gas, oil, and coal) but that they failed to utilize them effectively despite of an increase in the yields. Incomplete infrastructures and delay in the implementation of energy measures attracted attention. A higher priority was found attached to the upgrading of and investment in structures, renewal of facilities, and the intensification of management. The basic industries wanted the modernization of their facilities and processes. As for electric power consumption, Kazakhstan consumed 53.0-billion kWh, Uzbekistan 39.0-billion kWh, Turkmenistan 4.4-billion kWh, and Azerbaijan 16.4-billion kWh. In Uzbekistan, where the nonferrous metal refining industry led its national economy, the energy consumption rate was found to be quite high. There was a decrease in production in the cement making industry. (NEDO)

  16. Fiscal 1997 report on a feasibility study of international joint researches in the Asian region; 1997 nendo chosa hokokusho (Asia chiiki kokusai kyodo kenkyu kanosei chosa (4)hokokusho)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    It is thought that the energy consumption in Asian countries with ASEAN and China as center will a marked growth also in future together with a rapid economic growth, but harmony with environmental problems and economic growth is being taken as a serious problem. This is an important issue also to Japan from viewpoints of secure energy supply and regional response to the environmental issue. Accordingly, strongly desired is positive response to global environmental issues and promotion in development/spread of new energy/energy conservation in these countries. For the spread of the technologies, actual construction/operation of plants by joint researches with Japan are effective from aspects of personnel raising, technical improvement, PR effects, etc. This is also helpful for Japan in terms of technological development because Japan can also get the unobtainable data from the operation under appropriate conditions. This is a report on the studies of the trends of global environmental problems in Thailand, Indonesia, Malaysia, the Philippines, India and China. 3 figs., 72 tabs.

  17. Fiscal 1999 technical survey report. Basic survey of prioritized fields in South Asia (Bangladesh); 1999 nendo Minami Asia chiiki (Bangradesh) energy tashohi sangyo kiso chosa jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    For working out and proposing project themes optimum for the popularization of technologies for effectively utilizing energy in Bangladesh, a basic survey was conducted into Bangladeshi energy policy, trend of supply and demand, steelmaking industry, cement making industry, and chemical fertilizer making industry. Bangladesh in fiscal 1997 consumed 10-million tons of energy in terms of oil, 60% or more in natural gas (domestic), 20% in oil, and the remaining 20% in others. Approximately 15% of all the households were supplied with electric power. There was no integrated production plant in the steelmaking industry. Only one mill performed calcination in the cement making industry. The impact to be imposed by the steelmaking and cement making industries on Bangladeshi energy consumption would be small. Fertilizer making was an important industry outputting 2.6-million tons, responsible for 30% of natural gas consumption. The highest priority was given to facility rehabilitation and production enhancement, and there was little hope that energy conservation facilities/technologies would be introduced into the steelmaking and fertilizer making industries. It was possible, however, that the horizontal tube mill would be replaced by a vertical roller mill in the cement making industry. (NEDO)

  18. Airborne gamma-ray survey around the Negoro fault. 1; Negoro danso shuhen chiiki ni okeru kuchu {gamma} sen tansa. 1

    Energy Technology Data Exchange (ETDEWEB)

    Nakayama, E.; Kasuya, Y.; Hasegawa, H. [Aero Asahi Corp., Tokyo (Japan); Tsukuda, E. [Geological Survey of Japan, Tsukuba (Japan)

    1997-05-27

    An airborne gamma-ray survey was carried out to investigate the active fault system in the central structure line in the peripheral area of the city of Wakayama. At the same time, with an objective to enhance applicability of the airborne gamma-ray survey to active fault investigation, fundamental data were acquired and discussed. The measurement data were processed according to the standard method specified by IAEA. An ID-FFT filter and a nonlinear filter were employed to extract anomalous gamma-ray intensity values. The gamma-ray intensity distribution chart shows a noticeable positive anomalous area extending from the central part of the western edge to the north-east direction. This area agrees nearly well with the Negoro fault, but its peak portion is positioned slightly more to south than the position of the Negoro fault shown in existing data. The Sakuraike fault and the vicinity of the central structure line also show positive anomaly as a whole, particularly remarkably in the vicinity of their converging portion. However, differing from the vicinity of the Negoro fault, the areas are not extracted as an anomalous area which has directionality and extends in a line form. One of the factors for this would be that it is a fault in unsolidified deposits with low opening trend, differing from the one in solidified rocks. 1 fig., 3 tabs.

  19. Report on the project of drawing energy-saving visions for the Town of Sayo district; Sayomachi chiiki sho energy vision sakutei jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The survey/study is conducted to draw the district energy visions for Town of Sayo, Hyogo Pref. The town as a whole consumes energy at 1,269,040GJ/y, or 140,817MJ/y per capita, which is about 1.2 times larger than the nationwide average. By energy source, electric power accounts for 43%, and oil products 53%. The town as a whole emits carbon dioxide at 18,900,403kg-C/y, or 2,097kg-C/y per capita, which is lower than the nationwide average. The short-term (1 year or less), medium-term (3 year or less) and long-term (5 years or less) targets are drawn by employees of the town offices, citizens and entrepreneurs for energy-saving directions (saving of power for air conditioning by adjusting clothes, wider use of bicycles and public trains). Those facilities investigated for promoting energy saving include those for the town office buildings, solar energy system for producing hot water for town-operated swimming pool, and solar energy system and photovoltaic system for Harima Observatory Park. (NEDO)

  20. Report for fiscal 1979 on survey for development of overseas coals. Blau area, Kalimantan, Republic of Indonesia; 1979 nendo kaigaitan kaihatsu chosa hokokusho. Indonesia kyowakoku Kalimantan Blau chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1979-12-01

    Surveys including collection of information and materials relative to various conditions were performed on Republic of Indonesia, who is considered a promising coal supplying source for Japan in the future. Based on the assumption that supply capacity of coal will be expanded for thermal power plants, and large rise is anticipated in the coal price, and in order to solve the problems in the plan for migrating the population from Jawa Island where serious population concentration has been taking place, and in the regional promotion and development, the Indonesian Government has made open to foreign business entities in June 1978 the development of coal in the Kalimantan area. Nine corporations including those from Japan have participated in the international bid. The Indonesian Governments seeks in this project a product sharing system, rather than the acquisition of foreign currencies or the profit sharing from export earnings. The Government considers Japan as one of the coal exporting markets, and expects utilization of the Japan's advantageous institutional financing. It is urged that Japan should discuss the coal development in the Kalimantan area as part of dispersion of the coal supply sources upon considering the ocean transportation distance and the close economic relationship with the country. (NEDO)

  1. FY 1997 report on the field survey on country situations including efficient energy consumption. Vietnam; 1997 nendo chosa hokokusho (energy shohi koritsuka nado chiiki josei genchi chosa). Vietnam

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    Field survey was made on the current state of and issues on energy in Vietnam. In Vietnam, firewood is in wide use as non-commercial energy, and sums to a half of total energy consumption. Other energies such as hydroelectric power, petroleum, natural gas and coal are self-sustainable. Commercial energy consumption in 1995 is estimated at 10,070,000t in oil equivalent, which is broken down into 23% for coal, 42% in oil, 5% for natural gas and 30% for electricity. Abundant water resources will form the mainstay of future electric power supply. Commercial production of oil started in 1986 becoming an oil exporting country. Several promising natural gas fields were discovered as the result of the exploration by foreign capital. Coal deposits are estimated to be nearly 3.5 billion tons, and most of them are anthracite. Electric power demand is growing at a higher rate than the economic growth of Vietnam. The growth rate of electric power demand is set to be 1.3 times that of GDP. Since construction funds for new plants cannot be satisfied with the national budget and domestic investment alone, the country is expecting foreign capitals. 21 figs., 36 tabs.

  2. FY1997 geothermal development promotion survey. Development feasibility study 'Ashiro area'; 1997 nendo chinetsu kaihatsu sokushin chosa. Kaihatsu kanosei chosa (Ashiro chiiki) hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-12-01

    With regard to the Ashiro Town area in Iwate Prefecture, this paper reports the result of evaluations based on temperature and pressure logging after a lapse of an extended period of time (well No.2) and a steam jet test (well No.1). These activities were performed as the survey on promotion of geothermal development and survey on development feasibility in fiscal 1997. As a result of the overall analysis based on the present survey and ones in the past, the geothermal system model in the surveyed area may be conceived as follows: in both of the N7-AR-1 and N7-AR-2 wells drilled in the southern part of the surveyed area, temperature as high as 250 degrees C or higher was confirmed; the underground temperature is 200 degrees C or higher at an altitude level of zero meter and 250 degrees C at around -500 m, leading to a belief that the high temperature area spreads to south; as a fracture system holding geothermal fluid, a fault was identified at the N7-AR-1 well drilling depth of 1710 m; in the steam jet test, a geothermal reservoir (a shallow geothermal reservoir) was confirmed to exist; the geothermal fluid that has jetted out shows alkaline Na-SO{sub 4} type; and the deep geothermal reservoir has high temperature and is presumed to be in the two-phase condition, presenting promising factors as the geothermal resources. (NEDO)

  3. Report on the geothermal development promotion survey. No.C-2. Wasabizawa area; Chinetsu kaihatsu sokushin chosa hokokusho. No.C-2 Wasabizawa chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    The paper summed up the results of the survey of the geothermal development promotion survey - Wasabizawa area which was carried out in Yuzawa City and Ogachi Town, Akita Prefecture, from FY 1993 to FY 1996. In the survey, the following were conducted: surface survey such as geological alteration zone survey, fluid geochemical survey, gravity survey and electromagnetic exploration, temperature log by drilling 9 exploration wells, short-term/long-term jetting test, etc. Further, using the data obtained from these, analysis was made of geology/reservoir structure, thermal structure, geothermal water/hydraulic structure, geothermal system models, etc. As a result, a high-temperature (about 300 degrees C) zone promising as the geothermal development area was picked out, and the existence was confirmed of a dominant geothermal reservoir around the fault. In the jetting test, a total steam amount of 111.6 t/h was confirmed, and from the reservoir simulation, the result was obtained that there was a high possibility of 30-year power generation of 30MW/y. As a result of studying 'a power generation system by small-scale unit serial development' considering regional characteristics in the Wasabizawa area, the power generation cost (sending end, 15-year average) was estimated at approximately 9-10 yen/kWh. (NEDO)

  4. Report on the geothermal development promotion survey. No.B-7. Kuwanosawa area; Chinetsu kaihatsu sokushin chosa hokokusho. No. B-7 Kuwanosawa chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    The paper summed up the results of the geothermal development promotion survey B 'Kuwanosawa area' which was carried out in Yuzawa city, Akita prefecture, from FY 1998 to FY 1999. In the survey, the following were conducted for the comprehensive analysis: geology/alteration zone survey, gravity exploration, electromagnetic exploration, environmental effect survey, well geology survey by drilling structural boreholes of N11-KN-1 and N12-KN-2, cuttings test, temperature log, temperature recovery test, electrical log, water injection test, etc. The geology in the Kuwanosawa area is composed of Pre-neogene period basement rocks, Neogene system and Quaternary system. In this area, there were recognized no gush of geothermal fluids such as hot spring and fumarolic gas and no obvious geothermal manifestation such as high-temperature places and new geothermal alteration zones. Around N12-KN-2, there exists the geothermal water with comparatively high-temperature/high-Cl concentration which is similar to that in the Wasabizawa area, but how it flows is unknown because there was no lost circulation in the depths of the borehole. Around N11-KN-1, there exists the low-temperature/low-Cl concentration geothermal water originating in meteoric water, and therefore, the area can be a rechargeable area. The Kuwanosawa area is regarded as the periphery of the Wasabizawa-Akinomiya geothermal area. (NEDO)

  5. Investigation report on promotion of geothermal development. No. A-4, Mt. Kunbetsu area; Chinetsu kaihatsu sokushin chosa hokokusho. No.A-4 Kunbetsudake chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    This paper summarizes the results of investigations in the Mt. Kunbetsu area out of the investigations on promotion of geothermal development having been performed during the period from 1998 to 1999. The investigated area is an area located in the eastern Hokkaido with an area of about 300 km{sup 2}. The wide area investigation has discussed possibilities of existence of geothermal resources mainly from existence of high-temperature anomalies. The investigation has performed the following activities: fluid geochemical investigations on ten spring water sources, three spring gas sources, and five surface water points; gravity exploration at 153 measuring points; electromagnetic exploration at 52 measuring points; excavation of two heat flow rate wells (to depths of 997m and 995m), temperature logging, core cuttings tests, thermal hot water survey; other fluid inclusions tests, rock alteration age measurement; and temperature logging at Ryuun Shiretoko Spa. As a result of the investigations, it was estimated that, as the geothermal system model, the heat source is assumed to exist at a great depth below Mt. Kaibetsu; the spring water and the mineral spring water show diverse spring qualities because of mixing of meteoric water with fossil sea water; the fossil sea water and the meteoric water have been heated by thermal conduction; and the high-temperature zone is not spread to the positions of the investigation wells. (NEDO)

  6. Survey report on the development/utilization of petroleum substitution energy in the Pacific region; Taiheiyo chiiki sekiyu daitai energy kaihatsu riyo chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    The paper collected/analyzed data on petroleum substitution energy in the Pacific region. In fiscal 1996, in addition to collection of the existing energy data, conducted were collection of energy data made retroactive from 1971, and time-series collection of the energy related expanded data including energy related production statistics/social statistics, energy consumption amount by industry, power generation facilities, oil production facilities, and environmental data. As to petroleum substitution energy, the paper took up new energy such as hydroelectric, nuclear, geothermal, photovoltaic, and wind power generation, and commercial-base renewable energy. As a result of the analysis, the supply of petroleum substitution energy in the whole Pacific region has been stable, 6.6% during 1980-1990, 7.4% in the first half of the 1990s, largely over the primary energy supply. The supply of nuclear power was overwhelmingly large, followed by hydroelectric energy and geothermal/new energy. 15 figs., 23 tabs.

  7. Basic research report for drawing up regional new energy vision (provisional name) for Gunma Prefecture; Gunmaken chiiki shin energy vision (kasho) sakutei kiso chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    In view of relatively good solar radiation that the prefecture enjoys and of a result of the investigations of this time conducted into the amount of new energy in existence, it is concluded that a fairly large amount of solar energy is collectable as the regional new energy for Gunma Prefecture. As for wind power energy, since there is no proper location in the prefecture where the annual wind speed average of not less than 5m/s is available, the value generally accepted as necessary for wind power generation, it is inferred that conditions in the prefecture in general are disadvantageous when it comes to wind power generation. As for hydraulic energy of small and medium dimensions, a result of the investigations shows that in the prefecture this energy is next to solar energy in terms of the amount expected to be collectable. In this prefecture, the prefectural project bureau makes use of the rich water resources and generates electric power, and supplied approximately 850-million kWh in fiscal 1998. Since it is feared that it will be difficult to find new sites for power generation in the area now remaining to be developed, however, prudence should be exercised in estimating the expected collectable amount of hydraulic energy. (NEDO)

  8. Fiscal 2000 report on regional new energy vision formulation project for Yoron town; 2000 nendo Yoroncho chiiki shin energy vision sakutei nado jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A regional new energy vision has been formulated for Yoron town, Kagoshima Prefecture, which will serve as guidelines for building an Environmentally Friendly Island. Yoron town is a town of approximately 6,000 inhabitants as of fiscal 2000, its energy consumption is equivalent to 61,427 drums in terms of crude oil, and it receives electric power from Yoron Power Plant of Kyushu Electric Power Co., Inc. The results of activities conducted in this connection are summarized in seven chapters, which are (1) coping with new energy vision formulation (2), energy supply and demand, (3) estimation of existing amount of new energy, (4) basic policy toward new energy introduction, (5) projects for introducing new energy, (6) existing energy systems, and (7) reference materials. Named as projects are the construction of a water supply power source (for storage pump) for the Furusato district; power source for farmland irrigation pumps; power sources for the integrated sports park and municipal library; power source for a farmyard compost center; wind power, photovoltaic power, and combined power generation facilities for streetlights; and the utilization of bagasse. (NEDO)

  9. Report for fiscal 2000 on establishment of new energy vision in Fujino Town; 2000 nendo Fujino chiiki shin energy vision sakutei nado hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    With an objective to show specifically the efforts of working on new energies in Fujino Town in Kanagawa Prefecture, the existence quantity of new energies centering on the sun and woods was made clear to discuss the possibilities of utilizing them. Furthermore, investigations were performed on the energy demand and supply structure and possibilities of introducing new energies, and the specific new energy introduction plan was established. The activity achievements were put into order by the following six items: 1) the basic conception of the new energy vision, 2) various conditions of Fujino Town related to new energies, 3) energy demand and supply structure, 4) utilizable quantity of new energies, 5) toward the introduction of new energies, and 6) specific new energy facilities to discuss the introduction. Item 6 includes facilities to be built by defining model areas, facilities to discuss the full-swing introduction into facilities managed by the township, facilities to be discussed of full-swing introduction and to be composed in relation with the overall plan including large size hot water supply devices used at the spa, firewood boilers, a heat recovery system, and a large-scale photovoltaic power generation system. (NEDO)

  10. Geothermal development promotion survey report. No. 29. Upper reach region of Oita river; 1988-1990 chinetsu kaihatsu sokushin chosa hokokusho. No. 29 Oitagawa joryu chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1991-03-01

    The results of surveys conducted in the Oita river region, Oita Prefecture, in fiscal 1988-1989 are compiled in this report. Conducted were a geological/alteration zone survey, geochemical survey, electric prospecting (Schlumberger method), electromagnetic surveillance (simplified magnetotelluric method), electromagnetic surveillance (EMAP - Environmental Monitoring and Assessment Program method), heat flow rate survey, test boring, environmental impact survey, and so forth. Conclusions are mentioned below. It is inferred that the geothermal fluid results from groundwater originating in meteoric water, that the meteoric water takes many years to flow from the mountainous region into the ground where it is stored mainly in the Shonai stratum, that the stored water is warmed by heat from rocks in the neighborhood for development into a geothermal fluid, and that the geothermal fluid finally forms a hot spring water reservoir. Hot spring water reservoirs are found widely distributed in the basin of the Oita river. In view of the ground temperature distribution and the hot spring water geochemical temperature determined by structure boring, it is concluded that possibilities are quite low that there exists a high-temperature geothermal fluid usable for power generation. (NEDO)

  11. Report on regional new energy vision formulation project for Kahoku town, Kumamoto Prefecture; Kahokucho chiiki shin energy vision sakutei nado jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A regional new energy vision has been formulated for Kahoku town, Kumamoto Prefecture, for the realization of an energy resource circulation type regional community in which people earnestly strive to be symbiotic with nature. The results of related activities are shown in six chapters, which are (1) the prerequisites to the plan, (2) outline of the Kahoku town region, (3) surveys of the existing amount and expectedly available amount of new energy, (4) feasibility of new energy introduction, (5) assessment of new energy introduction plans and their effect, and (6) tasks to discharge, and policies to follow, for new energy introduction. In chapter (5), new energy introduction plans and introduction policies are shown. Plans are made for the incorporation of photovoltaic power facilities, small hydroelectric power facilities, and bamboo chip boilers into public facilities; the installation of 750 kW class wind power facilities; and the adoption of hybrid vehicles to serve as official special vehicles. It is expected that these plans when implemented will bring about a 1,400 t-c/year reduction in CO2 emissions. As for the introduction of wind power generation, the dimensions are determined by taking into consideration the prerequisites to a NEDO (New Energy and Industrial Technology Development Organization) grant. (NEDO)

  12. Tohoku Town developing with new energy. New energy vision of the Tohoku town area; 2001 nendo Tohoku machi chiiki shin energy vision. Shin energy de hiraku Tohoku machi

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Tohoku Town, Aomori Prefecture, an investigational study was conducted of the potential utilization of new energy, etc., and a vision was worked out. The population of Tohoku Town was 10,662 according to the results of the national census taken in 2000, which is slightly decreasing. The energy consumption amount of Tohoku Town was estimated at 280,977 Gcal/y, and the consumption of fossil fuel in the commercial/residential sector stands out. This is because a lot of kerosene is consumed in the area where it is cold. As measures to be taken for the new energy vision, the following were cited: initiative introduction of new energy to public facilities/preparation of a system toward the promotion of introduction, supply of thermal energy to townspeople/creation of the living environment using new energy, new energy use to activities of agricultural production/energy use of stockbreeding resource/energy use of forestry resource/potential study of the wind power generation, spread/education for townspeople/proprietors, preparation of places of bodily sensation/exchanges of new energy, training/support of activities of townspeople themselves, etc. (NEDO)

  13. Report on the project for working out the new energy vision of the Genkai town area; 2001 nendo Genkai machi chiiki shin energy vision sakutei tou jigyo hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Genkai Town, Fukuoka Prefecture, an investigational study was conducted of the estimated amount of energy demand of the town, existence amount of new energy, project for new energy introduction, etc., and a vision was worked out. The energy consumption amount of the town in FY 1999 was estimated at 834 TJ/y. By sector, it consisted of 299.31 TJ in the industrial sector, 282.08 TJ in the transportation sector and 252.78 TJ in the commercial/residential sector. By energy source, the rate of light oil was the highest, followed in order by gasoline, electric power, kerosene, LPG, etc. As the project for new energy introduction, the following were studied: preparation of disaster-prevention type photovoltaic power generation at shelter, etc.; introduction of photovoltaic power generation to kindergarten, elementary school, etc.; introduction of photovoltaic power generation to street light, tourist information center, etc.; introduction of wind power generation to public facilities; use of the waste cooking oil made into diesel fuel for official vehicle; introduction of cogeneration to the health welfare hall; solar light/heat utilization system for residential/industrial use; wind power generation business for business establishment. (NEDO)

  14. Report on new energy vision planning operation at Yachiyo Town; 2001 nendo Yachiyo cho chiiki shin energy vision sakutei gyomu hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    With an object of accelerating introduction of and uplifting consciousness on new energies at Yachiyo Town in Hyogo Prefecture, investigations and discussions were given on the energy consumption of the town, quantity of new energies in existence, and the new energy introduction projects, thus a new energy vision was established. Yachiyo Town consumes energy annually at 99,966 Gcal. By departments, the transportation department accounts for 46%, the industrial department for 24%, the civil household department for 16%, and the civil business department for 14%. By energy types, petroleum accounts for 69%, and electric power for 31%. The new energy introduction projects that have been discussed include introduction of photovoltaic power generation into the town office, public halls, primary and middle schools, and photovoltaic and micro-wind power generation for street lights, photovoltaic and wind power generation in the Garten Yachiyo, a city and farm town culture exchange facility, micro-hydroelectric power generation in Noma River, bio-mass energy utilizing forest resources and fabric wastes, clean energy fueled automobiles as public use cars, school buses and welfare buses, and small wind power and large photovoltaic power generation at Mt. Noma. (NEDO)

  15. Report of survey for formulating regional new energy vision for Okuma Town; 2001 nendo Okuma machi chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For promoting the introduction of new energy and for enhancing people's consciousness of such at Okuma Town, Fukushima Prefecture, surveys and studies were conducted involving the amount of energy needed by the town, the amount of new energy resources in existence, and new energy introduction projects, and a vision was formulated. The town demands 29,769 times 10{sup 7} kcal/year in energy comprising 44.5% for the industrial sector, 31.4% for the transportation sector, and 24.1% for the residential/commercial sector. As for energy source types, 25.7% is covered by electric power, 20.0% by gasoline, 16.2% by gas oil, and 15.3% by heavy oil, with the oil based sources responsible for 71.2%. New energy introduction projects were discussed, which included the introduction of photovoltaic power generation for a newly built children's hall, residential complexes, town managed housing, street lights and road signs, and for some other public facilities; introduction of cogeneration into enterprises present in the eastern industrial park and into hotels; and the introduction of natural gas fueled cogeneration. Studies were also made about public information, public relations, and support systems for implementation in Okuma Town. (NEDO)

  16. FY 2000 survey report on possibilities of the international joint study in the Asian region; 2000 nendo Asia chiiki kokusai kyodo kenkyu kanosei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Study was conducted of policies on energy spread, trends of energy development, etc. in energy conservation and substitution energy fields after the economic crisis in Asian countries, and the results were summed up. In Thailand, it is expected that the effective use of solar energy can be an effective means as a substitution energy. Further, the energy conservation by demand-side management, etc. is to be urgently taken. Indonesia is abundant in energy resources and does not take energy conservation measures very much. As to petroleum substituting energy, it is expected that the diversified use of coal, natural gas, hydroelectric power, geothermal energy, etc. will be advanced. In Malaysia, a policy in which energy is dispersed to natural gas, hydroelectric power and coal is being taken for reduction of the dependence upon petroleum. In India, joint projects with developed countries are proceeded with in the fields of electric power, energy conservation and renewable energy. In China, the policy for advancing new energy utilization is being taken that the thermal power generation is optimized, hydroelectric power generation is positively adopted, and nuclear power generation is moderately used, according to characteristics in the region. (NEDO)

  17. Report on the geothermal development promotion survey. No.34. Kaminoyu/Santai area; Chinetsu kaihatsu sokushin chosa hokokusho. No. 34 Kaminoyu Santai chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-03-01

    The paper summed up the results of the geothermal development promotion survey 'Kaminoyu/Santai area' which was carried out at Yakumo town, Yamakoshi county, and Mori town, Kayabe county, Hokkaido, from FY 1990 to FY 1992. In the survey, the following were conducted for the comprehensive analysis: surface survey such as geology/alteration zone survey, geochemical survey and gravity exploration, test to induce jetting of geothermal fluids by drilling 7 boreholes, temperature/pressure log, etc. The geological structure of this area is featured by the uplift structure/caldera structure and the anticline structure. In the wide-area geothermal system, it is thought that the low-temperature seawater permeates underground by mixture with meteoric water, is heated, and forms the deep geothermal water. It is thought that the hot spring water in this area is stored in the hot spring reservoir by mixture with the surface water, and it is gushing. In the area, the region which is regarded as favorable as passage/storage place of geothermal fluid is a region of 1.5km width extending linearly in the E-W direction from the Nigorikawa basin to Kaminoyu. Further, it is thought that fractures were unfavorably developed in the Santai/Kaminoyu area. (NEDO)

  18. Origin of allochthonous limestone bodies of the miocene Megami formation, Kakegawa district, Shizuoka prefecture. Shizuokaken Kakegawa chiiki no chushinto megamiso ni fukumareru ichisei sekkaigan gantai no kigen

    Energy Technology Data Exchange (ETDEWEB)

    Nakamori, T; Iryu, Y; Mori, K [Tohoku Univ., Sendai (Japan). Faculty of Science; Sasazawa, K [The Yomiuri Shimbun, Sendai (Japan). Tohoku General Bureau

    1991-12-15

    The Tertiary limestone bodies represented by Megami Hill and Ogami Hill are distributed in the Kakegawa district in the central portion of Shizuoka Prefecture, these limestone bodies are those originated from coral reefs and are contained in the Megami formation composed of sandstone, mudstone and tuff. However, concerning the limestone bodies of the Megami formation, there are, inter alia, the opinion that they are coral reef sediments on the spot and the opinion that they are foreign rocks, but neither has any firm concrete supporting evidence. In this paper, the sedimentation ages and the sedimentary locations of limestone and sandstone {center dot} mudstone composing the Megami formation were classified and the process, through which limestone was transformed up to its state now observed, was studied. The above study was done by a detailed survey of local rock facies, comparison of age of each rock facies through detection of reference fossils and other measures. A part of the conclusion is shown below: The limestone bodies of the Megami formation is discontinuous with the surrounding sandstone {center dot} mudstone and their strike dip is not harmonious. These limestone bodies are allochthonous rock blocks which collapsed after their sedimentation and mixed with the deep sea sediments (sandstone and mudstone). 37 refs., 4 figs., 4 tabs.

  19. Evaluation report on the design of solid catalysts for saving energy in petrochemical industry; Sekiyu kagaku no tame no sho energy gata kotai shokubai sekkei hyoka hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The aim is to improve on hydrocarbon oxidation which is a difficult reaction (analogous to combustion and having potential for heat generation or explosion, with restrictions therefore imposed on reaction conditions) by use of a catalyst supporting porous membrane. The fiscal 1999-2000 results are stated. In this effort, the target reaction is promoted by a catalyst supported by a porous membrane while combustion is controlled by the said porous membrane that separates oxygen and hydrocarbon from each other. Such a design was not available since a catalyst supporting inorganic membrane withstanding so high a temperature was difficult to prepare. Recently, however, a porous membrane with its pores regulated to microstructural dimensions was developed. The activity of a Pd membrane catalyst is evaluated in view of the degree of oxidation of propylene, cyclohexene, and benzene. It is then found that hydrogen that has passed the Pd membrane participates in the reaction. In a propylene oxidation test using a membrane type Ag/Al{sub 2}O{sub 3} catalyst, it is found that acetone is generated and that oxygen after passing through the membrane participates in the reaction. Methods are studied for preparing a metal membrane in which the catalyst system comprises a catalyst supporting porous membrane and a Pd membrane. In the fabrication of metal membranes, a highly selective hydrogen permeable membrane is stably prepared by the thermal CVD (chemical vapor deposition) method. (NEDO)

  20. Development of multi-frequency array induction logging (MAIL) tool. 3; Multi shuhasu array gata induction kenso tool (MAIL) no kaihatsu (genchi shiken data no ichijigen kaiseki). 3

    Energy Technology Data Exchange (ETDEWEB)

    Sato, T; Osato, K; Takasugi, S [Geothermal Energy Research and Development Co. Ltd., Tokyo (Japan); Uchida, T [New Energy and Industrial Technology Development Organization, Tokyo, (Japan)

    1996-05-01

    A field test was carried out in 1995 on a deep-seated geothermal investigation and reduction well WDR (in Kakkonda, Shizuku-ishi, Iwate Prefecture) by utilizing a multi-frequency array induction logging tool which is under development by NEDO. This paper reports results of analyzing the acquired data. With the WDR wells, an investigation has been conducted to a drilling depth of 330 m. Three frequencies in a range from 3 to 24 kHz provided relatively good data, but data with 42 kHz had poor quality that cannot be utilized for the analysis. Precise device calibration is difficult on data acquired from three array type vertical component magnetometers (the difficulty may be due to a signal line from a transmitter passing on the magnetometer side). Because of this difficulty, analysis using one-dimensional inversion program was performed by using ratio of the amplitude for each frequency and phase difference as input data. The analysis allowed to have derived a result that corresponds to the ground bed structure. 5 refs., 8 figs., 3 tabs.

  1. Influência do teor de água e da digestibilidade de alimentos industrializados sobre o balanço hídrico de gatas

    Directory of Open Access Journals (Sweden)

    Aulus Cavalieri Carciofi

    2005-12-01

    Full Text Available Dietary adjustment has been studied for the control and prevention of the Feline Lower Urinary Tract Disease. This work studied the influence of the amount of water and food digestibility on water intake and excretion (urinary and fecal. Ten adult female cats housed in metabolic cages were used. Four treatments were tested: low price dry food, low price dry food with 50% added water, super-premium dry food and canned food. Water intake with food and by drinking, fecal and urinary water excretion and food's digestibility coefficient were determined, in a Latin square experimental design with repetitions over time. The results were submitted to variance analysis; to Tukey's test to compare means and to Pearson's Correlation to test the association between variables (p<0.05. Moist food led to the highest water intake and the largest urine volume with the lowest density. The cats balanced the higher water intake with food by drinking less water. Adding 50% water to the food did not result in a higher total water intake and urine excretion. The lowest consumption of dry matter and highest food digestibility resulted in lower fecal water excretion. Dry foods with high and low digestibility had a urine:feces water excretion rate of 0.7:1 and 1.6:1, respectively. Among the nutrients, fat intake had a positive correlation with the urine volume. Foods with higher digestibility resulted in lower fecal water loss. Canned food (high water:dry matter ratio led to the highest total water intake and urinary volume excretion, and lower urinary density.

  2. Crossflow type silicon microchannel substrate monodispersion oil-in-water emulsion manufacture; Kurosufuro gata shirikon maikuro chaneru kiban wo mochiita tanbunsan suchuyu emarushon no sakusei

    Energy Technology Data Exchange (ETDEWEB)

    Kawakatsu, Takahiro [Tohoku University, Miyagi (Japan). Graduate School; Komori, Hideai; Najima, Mitsutashi; Kikuchi, Yuji; Yonemoto, Toshikuni

    1999-05-05

    The new technique, which continuously produced the monodispersion oil-in-water (0/W) emulsion using the crossflow type silicon microchannel substrate, was developed. On the silicon monocrystal substrate, the watercourse as the liquid of the continuous phase flowed was produced, and the column of the equal slit of the size in both walls of the watercourse was precisely processed. By closing the upper part in the slit by the clamp of the flat glass board in the microchannel substrate, the microchannel column was formed. Through the microchannel, the oil droplet in which the size was even was formed by sending out the oil (triolein) in the water (0.3wt% sodium lauryl sulfate aqueous solution) of continuous phase which is flowing in respect of the watercourse. The size of the oil droplet is greatly dependent on the structure of the microchannel regulated by microchannel width, microchannel height and terrace length (the even part of which the microchannel exit was equipped). Monodispersion emulsion of 16,20 and 48 {mu}m at the average droplet diameter was formed by using microchannel substrate of the three types of which the structure differs. Droplet diameter decreased, when the substrate which formed large droplet of 48 {mu}m in which the water current quantity is 1.4x10{sup -2}mLmin{sup -1} was used, when the flow rate increased. However, there was no a flow rate at droplet diameter, even if it was made to change from 1.4x10{sup -2} to 2.4mLmin{sup -1}, 16 {mu}m 20 {mu}m small change. In all cases, the droplet size distribution was narrow, and the geometry standard deviation was under 1.03. (translated by NEDO)

  3. Report on investigation in fiscal 2000 of industrial technology exchange with international networking organizations; 2000 nendo kokusaitekina network gata soshiki tono sangyo gijutsu koryu chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    With an objective to promote exchange of industrial technologies, investigations and analyses were made on identification of the current status of networking organizations in different countries working as windows for industrial technology exchange, and on the actual status of technology commercialization methods in overseas incubators. Activities were taken in the following three fields: 1) the current status of networking organizations and incubators in different countries, 2) typology of technology commercialization, technical fields, and success factors, and 3) possibility of the use of technology information in the networking organizations. In Item 1), investigations were performed on the current status and actual activity status of the networking organizations including research parks and individual incubators intended of information exchange, mainly in the United States, UK, and Finland. In Item 2), considerations are given on the points related to technology incubation based on the information about the networking organizations and incubators in each country, and the way the industry-academia cooperation should be. In Item 3), discussions were given on the roles of the networking organizations and the possibility of utilization of technological information in the networking organizations in Japan. (NEDO)

  4. Feasibility study on the demonstrative test on the hybrid mini hydroelectric power generation technology. 2; Hybrid gata mini suiryoku hatsuden gijutsu ni kakawaru jissho shiken kanosei chosa. 2

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    A study was made for the purpose of electrification of unelectrified zones of the mountainous areas in developing countries using extremely small hydroelectric power facilities. The target for this project is a development of a micro hydroelectric power system with a size of about 5kW, which is estimated regarding that 1 village has 80 houses and each house needs electricity of 60W. In the study, the low-head system using a weir of irrigation channels in Subang pref. of West Java state was selected by the evaluation of access, stability of flow rate, natural conditions, etc. The hydroelectric power plant is of a flow-in method in which water is taken from the left bank and is injected/discharged to the downstream of the left bank. As the hybrid complementary power source, hybrid battery with a two-hour charging time at peak and a capacity of 3.5kWh was considered. When estimating the construction cost of the hybrid micro hydroelectric power system and equalizing by durable years, the operational cost per kW is 15 times higher than the benefits which local people receive. It was judged to be difficult to say that the micro hydroelectric power system is economically feasible. It was predicted that the financial profit during the demonstrative test is good, according to a trial calculation of income from power rates and the operational cost. 18 refs., 90 figs., 53 tabs.

  5. Numerical estimation of stability and improvement for coolant passage design in Hollow type CICC; Hollow gata CICC no reikyaku ryuro kaizen to anteisei no suchiteki kento

    Energy Technology Data Exchange (ETDEWEB)

    Miyauchi, M. [Yamagata Univ., Yamagata (Japan); Yoshiki, H. [The Univ. of Tokyo, Tokyo (Japan)

    1999-06-07

    The Hollow type CICC has a central channel (Hole) and is separated from surrounding bundle by a spiral tape with a gap, heat of a strand can effectively be convected to the Hole zone. However, even strong convection currents are induced in two ends of a thermal generating zone, but the heat is accumulated in the central part, and stability margin drops while relatively a long time heat has input for about 100 msec. In this study, the helium deposit inside the heating zone is reduced by a little design changing of the spiral tape that separates the bundle and the hole and efforts of improving stability of low energy density against heating are attempted. The instability caused by low energy and a long time heat invasion of the CICC appears also in simulation of Zanino at el. using a MITHRANDIR code. At this time, a method for calculating overflow of helium flowing to the bundle/Hole taking references of a branch current model and a joint flow model that are used in engine simulations is developed. (NEDO)

  6. Improvement of strength and toughness of SKD11 type cold work tool steel; SKD 11 gata reikan koguko no kyojinsei no kaizen

    Energy Technology Data Exchange (ETDEWEB)

    Fukaura, K.; Sunada, H.; Yokoyama, Y. [Himeji Inst. of Technology, Hyogo (Japan); Teramoto, K. [Himeji Inst. of Technology, Hyogo (Japan). Graduate School| Sanyo Special Steel Co. Ltd., Hyogo (Japan)

    1998-03-01

    Toughness and wear resistance are the factors which affect on the lifetime of cold work tool steels importantly. Generally, both properties show the contrary characteristics. The evaluation of tool steel materials has been carried out focusing on the strength and wear resistance mainly. However, owing to the rapid progress of recent plastic working techniques, usage environments are under the severe conditions more and more. Therefore, it is expected to develop the high reliable cold work tool steels which balanced with toughness including wear resistance. In this study, the strength and toughness of a newly developed 0.8C-8Cr cold work tool steel whose composition was controlled to suppress the precipitation of massive eutectic M7C3 carbides were investigated with reference to microstructure and were compared with conventional SKD11. The toughness was evaluated by the area under the stress-strain curve. As a result, it was clarified that the tensile strength of the newly developed steel designated as Mod. SKD 11 was about 400 MPa higher and the toughness was 1.8 times larger than that of SKD 11 throughout a wide range of tempering temperatures and so forth. 17 refs., 13 figs., 1 tab.

  7. Separation of polyunsaturated fatty acid esters by flowing liquid membrane with porous partition.; Kakumaku gata ryudo ekimaku ni yoru kodo fuhowa shibosan esuteru no bunri

    Energy Technology Data Exchange (ETDEWEB)

    Yokosawa, R.; Nii, S.; Takahashi, K. [Nagoya Univ., Nagoya (Japan). Depertment of Chemical Engineering; Misawa, Y. [Harima Chemicals, Inc., Osaka (Japan)

    2000-07-10

    A laboratory-constructed flowing liquid membrane apparatus with porous partition (FLM) was applied to the separation of such polyunsaturated fatty acid esters (PUFA-Ets) as eicosapentaenoic acid ethylester (EPA-Et) and docosahexaenoic acid ethylester (DHA-Et) at 293 K. The hydrophilic porous membrane was used as a partition, and 2 M AgNO{sub 3} aqueous solution was used as a membrane liquid. By using dodecane as a feed diluent and m-xylene as a recovery solution, PUFA-Ets were successfully separated from mixture of fatty acid esters. The recovery fraction of PUFA-Ets and the separation degree between DHA-Et and EPA-Et were investigated ; it increases with the velocity of membrane liquid and the flow path length in the module, while it decreased with the velocity of feed and recovery solution. The recovery fraction of DHA-Et reachs 60% with only 8 s-retention time by the continuous operation. The concentrations of the DHA-Et at the outlet of the module were simulated based on the mass transfer model, which gave nearly 85% recovery fraction with 30 s-retention time. These results show that a stable operation, a high recovery and a high recovery and a high throughput are realized by the FLM. (author)

  8. Development of multi-frequency array induction logging (MAIL) tool. Part 4; Multi shuhasu array gata induction kenso (MAIL) tool no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    Sato, T.; Otsuka, K.; Takasugi, S. [GERD Geothermal Energy Research and Development Co. Ltd., Tokyo (Japan); Uchida, T. [New Energy and Industrial Technology Development Organization, Tokyo, (Japan)

    1997-05-27

    NEDO is now carrying out `the deep-seated geothermal resource survey` with the aim of surveying deep geothermal resource lying 3,000m below sea level. `The development of resistivity use investigation technology` being carried out as element technology for this survey conducts well surveys and earth surface-well surveys using the drilled deep wells. By analyzing these surveys together with electromagnetic method surveys including the MT method carried out on the earth surface, the survey was aimed at grasping in high resolution resistivity structures from the shallow underground to the deep underground. The multi-frequency array induction logging being researched as the well survey is a method to grasp resistivity structures within a radius of several meters of the well using the electromagnetic logging method. The paper reported the field test conducted this time using the improved tool. A comparison of the data obtained in the test with the results of the normal logging showed consistency, and therefore, it showed that the tool itself fully grasped responses from strata. 3 refs., 5 figs., 1 tab.

  9. Basic research on the development of 'intelligent-type' humidity control materials; Intelligent gata choshitsu zairyo no kaihatsu ni kansuru kiso kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    Tomura, S.; Maeda, M.; Inukai, K.; Ohashi, F.; Suzuki, M.; Suzuki, K.; Shibasaki, Y. [National Industrial Research Institute of Nagoya,Nagoya (Japan)

    2000-10-25

    The water vapor adsorption desorption isotherms of purified and/or synthesized sepiolite, allophane, diatomite, selectively leached kaolin and mesoporous silica were measured to develop humidity control materials in living environments. Based on Kelvin's capillary condensation theory, suitable pore diameters for controlling relative humidity at 40 and 70% were calculated to be 3.2 and 7.4 nm, respectively. Wakkanai diatomite, purified allophane, selectively leached kaolin and mesoporous silica have suitable pore diameters and high water adsorptivity, and were considered as candidates for humidity self-control materials. Among these materials, mesoporous silica formed as a tile showed the best performance as a humidity control material in desiccator and model house levels. (author)

  10. Center post type cyclo-speed reducer for sludge scrapers for sedimentation ponds; Chindenchi odei kakiyosekiyo center post gata saikuro {reg_sign} gensokuki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-04-20

    This equipment is a compact center post type cyclo-speed reducer composed by combining an internal gear with the cyclo-speed reducer which is in wide use for water treatment equipment. The features are as follows: (1) Drastic size reduction by adopting a direct-coupled structure of the vertical cyclo-speed reducer and an internal gear, (2) High efficiency by combining an internal gear with the cyclo-speed reducer, (3) Easy maintenance and inspection by a simple speed-reducing mechanism. (4) Cyclo-speed reducer with a torque limiter being in wide use as safety device, (5) Torque limiters corresponding to the specifications of various cyclo-speed reducers, and (6) Low price and short delivery time by modification from a previous revolving bearing to a rail rolling bearing, and standardization of main parts. (translated by NEDO)

  11. Fiscal 2000 regional consortium research and development project - regional new technology creation research and development. Development of micro-array for next generation gene analysis (1st fiscal year); 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki shingijutsu soshutsu kenkyu kaihatsu seika hokokusho. Jisedai idenshi kaiseki micro array no kaihatsu (daiichi nendo)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Efforts are under way to construct a novel DNA (deoxyribonucleic acid) micro-array for gene diagnosis on the basis of technologies of laser scan type manipulation, nanometric position detection, and micro-machining. Using these technologies, structural changes to accompany reactions induced in the probe DNA deposited on an array are detected for the identification of the DNA. Activities are conducted in the four fields of (1) the study of probe DNA fixation technology, (2) development of an optical detection system, (3) detailed check of DNA micro-array performance evaluation technologies, and (4) a comprehensive survey. In field (1), gold colloid modified DNA molecules are designed and evaluated, and the fixation of DNA to substrates and technologies for integration are studied. In field (2), the gold colloid modified DNA is fixed on a thin gold film, and then a surface plasmon resonance (SPR) is observed in the wake of hybridization. Furthermore, a Brownian motion is observed of the metal particles fixed on a glass substrate via DNA. (NEDO)

  12. Fiscal 2000 regional consortium research and development project - regional new technology creation research and development. Development of bio-recycling technology for bio-wastes; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki shingijutsu soshutsu kenkyu kaihatsu seika hokokusho. Seibutsukei haikibutsu no bio recycle gijutsu no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Efforts are exerted to develop a closed brewing system for 'shochu (a Japanese distilled liquor)' and vinegar and a non-combustion type thermal recycling system based on the methane fermentation of farm animal's excretion and sludge. Activities are conducted in the three fields of (1) the development of a zero emission shochu and vinegar brewing process, (2) development of a non-combustion type thermal recycling method, and (3) a commercialization plan. Studied in field (1) are the brewing of 'shochu' by a new process (kaeshi-shikomi), preparation of vinegar out of the lees of 'shochu' brewing, evaluation of the physiological activity of vinegar and 'shochu' lees, cost efficiency evaluation of zero emission 'shochu' and vinegar brewing processes, and the like. In field (2), the methane fermentation conditions for garbage, sludge, and farm animals' excretion are studied, and a non-combustion type thermal recycling process and the like are evaluated for cost efficiency. In field (3), marketability is studied for vinegar made by fermentation, actualities of the recycling of bio-wastes and energy recovery therefrom are examined, and marketability is studied for regional distributed power generation. (NEDO)

  13. New energy vision of Rokunohe Town. Maple green plan (Town creation using the riches of Nature and industries of the area); 2001 nendo Rokunohe machi chiiki shin energy vision. Meipuru Green keikaku - Yutaka na shizen to chiiki no sangyo wo ikashita machidukuri

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Rokunohe Town, Aomori Prefecture, an investigational study was conducted of the state of energy supply/demand, existence amount of new energy, introduction of new energy, etc., and a vision was worked out. The population of Rokunohe Town was 10,481 according to the results of the national census taken in 2000, which is slightly decreasing. The energy consumption amount of the town is 16,063.6t in oil conversion, and the consumption depends on fossil fuels such as electric power, gasoline, light oil, heavy oil and LPG gas. For the action plan for introduction of new energy, the following were cited: field study of the photovoltaic power generation, preparation of the shopping street/school street which are safe and unfrozen (snow melting of sidewalk), potential study of the agricultural development of snow/ice energy, use of the agricultural waste such as animal feces and waste vegetable (study of introduction of small-scale bio-system), promotion of recycle of cooking oil (study of the use of clean energy vehicles for official vehicles and use of diesel substituting fuel), study of use of energy crops for the idle field, various education projects, etc. (NEDO)

  14. Fiscal 2000 survey report on Asia/Pacific Regional Environment Problems Researcher Exchange Promotion Projects. Construction of Environmental Technology Research Network in the Asia/Pacific Region; 2000 nendo Asia Taiheiyo chiiki kankyo mondai kenkyusha koryu sokushin jigyo hokokusho. Asia/Taiheiyo chiiki kankyo gijutsu kenkyu kaihatsu network kochiku chosa

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    For the purpose of developing and popularizing environment conservation technologies in the Asia/Pacific region, activities were conducted for Environmental Technology Research Network in the Asia/Pacific Area (ETERNET-APR). In this fiscal year, under the motto of Expansion of International Collaborative Researches on Environmental Technology, a 7th international workshop was held in Yokkaichi City, Mie Prefecture, with the attendance of approximately 60 people in Japan and from abroad. Researchers from overseas, in addition, attended a 10-day training program at the National Institute for Resources and Development and others in preparation for the embodiment of international collaborative research. A survey was conducted overseas for the promotion of database utilization and registration and for grasping the problems intrinsic to the respective research institutes and problems to occur at every stage of development. People's Republic of China was visited, where a miniscale workshop was held, study tours were made, and opinions were exchanged. It was actually felt, at the places that were visited, that ETERNET-APR was certainly in function in various fields. (NEDO)

  15. FY 2000 report on the results of the regional consortium R and D project - Regional new technology creation R and D. First year report. Functional molds with highly polished diamond coatings; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki shingijutsu soshutsu kenkyu kaihatsu. Kyomen diamond maku wo yusuru kokino kanagata (shonendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The development was proceeded with of the technology to form the uniform diamond coating on mold with the complicated shape and also to highly polish it by a new chemical polishing method. The new chemical polishing method is a method using TiAlX intermetallic compounds as grindstone. Reaction with each other of Ti and C by frictional heat expedites diamond polishing. 1) development of the controlling technology of diamond coating interface; 2) development of the 3-D diamond polishing system; 3) demonstrative test on diamond coating; 4) study of a possibility of the commercialization. 1) was divided into the development of the controlling technology of diamond coating interface and the survey of super-hard alloys suitable for diamond coating formation. In the latter, it was found out that the highly Co containing particular WC super-hard alloys were bad in adhesiveness to diamond coating. Therefore, studies were made on the manufacturing of super-hard alloys which use coarse WC only near the surface and does not have much Co and on the use of SiC excellent in adhesiveness both to super-hard alloys and diamond for intermediate layer. (NEDO)

  16. FY 2000 report on the results of the regional consortium R and D project - Regional consortium energy. First year report. Development of a next generation biopolymer information measuring system based on the nano channel electrophoresis; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki consortium energy. Nano channel denki eido ni motozuku jisedai seitai kobunshi joho keisoku system no kaihatsu (dai 1 nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    On the basis of the nano channel electrophoresis technology, the construction was proceeded with of a system which integrates the basic process such as reaction, separation and detection needed for measuring biopolymer information and analyzes a number of samples simultaneously and with high accuracy/high sensitivity. Studies were made in the following three fields: 1) development of the basement technology on extraction/amplification/separation/detection of biopolymer; 2) development of micro-processing technology and system design and study on the development of device; 3) comprehensive investigational study. In 1), developmental technology is divided into the following: technology to effectively fraction/refine biopolymer from biological samples, technology for recognition control and manipulation of biopolymer, technology of amplification use micro-reaction of biopolymer, multiple selection technology of biopolymer using the specific molecular recognition, and technology for ultra-high speed separation of biopolymer. As to the multiple selection technology, DNA terminal recognizing hemin is altered by thiol group and connected to the surface of electrode to make an electrode type hemin recognition device. (NEDO)

  17. Fiscal 2000 regional consortium research and development project - regional new technology creation research and development. Massive rapid identification of gene functions; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki shingijutsu soshutsu kenkyu kaihatsu seika hokokusho. Idenshi kino no tairyo jinsoku dotei ni kansuru kenkyu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Efforts are exerted to develop a system for massive and rapid identification of unknown genes through the utilization of techniques based on the unusual membrane fusion characteristics of HVJ (hemagglutinating virus of Japan), which enables the introduction of genes efficiently and uniformly into cultured cells and living organs (development into a kit). Activities are conducted in the three fields of (1) the development of a method for introducing genes using an HVJ envelop vector, (2) a survey of kit marketing and cold storage facilities in the selling and distribution of the kit, and (3) a comprehensive survey. In the field of (1), studies are conducted for the establishment of methods for HVJ envelop vector production and refining, the establishment of an optimized method for encapsulating genes into the HVJ envelop vector, and the introduction of genes into cultured central neurons using the HVJ envelop vector system. A refining method has been established, which comprises the inoculation of HVJ into eggs, 4-day culture, egg cutting, liquid sampling (chorioallantoic liquid), inactivation (addition of alkylating agent), filtration, enrichment (ultrafiltration), and an anion exchange chromatogram. (NEDO)

  18. Report of the results of the fiscal 1997 regional consortium R and D project. Regional consortium energy field / Development of the plasma use surface treatment process by in-situ control (first fiscal year); 1997 nendo chiiki consortium kenkyu kaihatsu jigyo. Chiiki consortium energy bun`ya / in-situ seigyo ni yoru plasma riyo hyohi shori process no kaihatsu (daiichi nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    The paper described the fiscal 1997 result of the development. To know of in-plasma phenomena such as carburization and nitriding, a basic plasma experimental device was fabricated for quantitative measurement of reaction activity species. For the study of reaction control between plasma and substrate, a rotary analyzer type ellipsometer was fabricated as a method to detect composition and thickness of the deposit on the substrate surface. For He gas cooling after carburization and hardening, basic specifications for He gas refining/circulating system were confirmed. For perfect non-hazardous processing of exhaust gas from plasma carburization furnace, conducted was the thermodynamic computation of the process. Priority in order of the functions to be possessed as specifications for basic design of mini plant is plasma carburization, He gas cooling, and in-situ measurement. To make the most of the plasma use surface treatment as substitutes for expensive alloy elements, sliding parts/die-cast mold raw materials were carburized to measure the hardness. The Cr carbide coating technology by plasma CVD is also under study as an application example except carburization. 47 refs., 59 figs., 31 tabs.

  19. Report of the results of the fiscal 1997 regional consortium R and D project. Regional consortium energy field / R and D of a task adaptation type group architecture transfer robot system, TRIPTERS (first fiscal year); 1997 nendo chiiki consortium kenkyu kaihatsu jigyo. Chiiki consortium energy bun`ya / task tekigogata gun kosei hanso robot system TRIPTERS no kaihatsu kenkyu (daiichi nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    The paper stated the fiscal 1997 result of the development of a task adaptation type group architecture transfer robot system (TRIPTERS) which can cope with changes in carrying task rapidly and flexibly. R and D were conducted mainly of various functional modules, the operation management technology which enables group architecture, and the basic design of a standardized transfer robot. As to the positioning, studied were the construction of the basic hardware of laser position measuring device, and the application method. Concerning the cooperative carrying, conducted were securing of positioning accuracy of matters to be carried, high speed heavy transfer control, and design of dead reckoning system. Relating to the operation management, passable areas were divided into more than one zones, and the effective path reservation method was constructed so that one and the same zone is not occupied. As to the environmental recognition/obstacle avoidance, developed were actual hour/distance acquisition equipment, and autonomous cars running according to the directions of the color sign recognition system by stereo CCD camera. Also conducted were the development of methods to recognize the distance to obstacles and to discriminate areas, the development of mobile sensor, and the basic experiment on running of the demonstration machine. 44 refs., 153 figs., 15 tabs.

  20. FY 2000 report on the results of the regional consortium R and D project - Regional consortium energy R and D. Development of new vacuum ultraviolet area optical materials realizing next generation short wavelength optical lithography; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki consortium energy kenkyu kaihatsu. Jisedai tanhacho hikari lithography wo jitsugensuru shinku shigaiiki kogaku zairyo no kaihatsu seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    As materials for next generation lithography use optical device using short wavelength light sources such as F{sub 2} and Ar{sub 2}, the development was proceeded with of fluoride monocrystal materials and 12-inch class large/high quality monocrystal production technology. Studies were made in the following five fields: 1) proposal/design of new materials and the heightening of performance: 2) establishment of the large/high quality crystal production method; 3) evaluation of optical properties and elucidation of micro-defect formation mechanism; 4) comprehensive investigational research; 5) study of the evaluation technology by vacuum ultraviolet area pulse light. In 1), for the development of the optimum materials, a lot of materials were tried to be monocrystallized, and the permeability was estimated by measuring the reflectance in the vacuum ultraviolet area. As to LiCaAlF{sub 6}, monocrystal with 1-inch diameter was made by the Bridgman method. In 2), studies were made of conditions for large crystal growth by the pull method, large crystal growth by the Bridgman method, and the structure of production equipment for crystals with larger diameter. (NEDO)

  1. Fiscal 2000 regional consortium research and development project - regional new technology creation research and development. Development of bio-recycling technology for bio-wastes; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki shingijutsu soshutsu kenkyu kaihatsu seika hokokusho. Seibutsukei haikibutsu no bio recycle gijutsu no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Efforts are exerted to develop a closed brewing system for 'shochu (a Japanese distilled liquor)' and vinegar and a non-combustion type thermal recycling system based on the methane fermentation of farm animal's excretion and sludge. Activities are conducted in the three fields of (1) the development of a zero emission shochu and vinegar brewing process, (2) development of a non-combustion type thermal recycling method, and (3) a commercialization plan. Studied in field (1) are the brewing of 'shochu' by a new process (kaeshi-shikomi), preparation of vinegar out of the lees of 'shochu' brewing, evaluation of the physiological activity of vinegar and 'shochu' lees, cost efficiency evaluation of zero emission 'shochu' and vinegar brewing processes, and the like. In field (2), the methane fermentation conditions for garbage, sludge, and farm animals' excretion are studied, and a non-combustion type thermal recycling process and the like are evaluated for cost efficiency. In field (3), marketability is studied for vinegar made by fermentation, actualities of the recycling of bio-wastes and energy recovery therefrom are examined, and marketability is studied for regional distributed power generation. (NEDO)

  2. Report of the results of the fiscal 1997 regional consortium R and D project. Regional consortium energy field/R and D high performance flat panel display technology (first fiscal year); 1997 nendo chiiki consortium kenkyu kaihatsu jigyo. Chiiki consortium energy bun`ya / koseino flat panel display gijutsu no sogo kaihatsu kenkyu (daiichi nendo ) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    One of the subjects in technology supporting the highly information-oriented society which will develop and diversify toward the 21st century is the construction of high grade man/machine interface. For it, high precision/high luminance/energy saving/thin plane displays are strongly requested. This R and D is to indicate models of systematical development in the region of element technology individually existing in the Shikoku area by forming a regional consortium in the industry/universities/government. Creation of new industries by gathering display related enterprises is a first step in a plan to realize `Display Island Shikoku.` As a concrete target, with the use of high-tech diamond semiconducting technology, a development is conducted of the high performance flat panel display using the negative electron affinity (NEA) electron emitter which drastically solves the problems such as luminance, visibility angle and response speed, the subjects on the commercialized liquid crystal flat panel display. 16 refs., 45 figs., 8 tabs.

  3. FY 2000 report on the results of the regional consortium R and D project - Regional consortium field. First year report. R and D of highly integrated micro-protein reactor array system; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki consortium bun'ya. Koshusekigata micro protein reactor array system no kenkyu kaihatsu (dai 1 nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    By micro-processing, micro-protein reactor array is fabricated, and inside it a very trace of gene is amplified by PCR (polymerase chain reaction) and then was amplified by adding the transcription/translation reaction mixture to it. By causing the protein synthesis reaction using the amplified gene, a highly integrated protein library is constructed. For it, the development of a new micro-protein reactor array system ({mu} PRAS) was proceeded with. Studies were made in the following 8 fields: 1) materials of micro reactor array and the manufacturing method; 2) development of the element unit of {mu} PRAS; 3) trial manufacture of micro reactor array chip and the evaluation; 4) trial manufacture of the micro-domain high-speed DNA manipulation system; 5) method to control non-specific DNA amplification in unimolecular PCR; 6) basic study of technology of molecular evolution of degrading enzymes of degradation-resistant substances such as dioxin; 7) method to construct the enzyme lipase library and computer simulation; 8) optimization of a system to synthesize optical activity useful compounds using enzyme lipase. (NEDO)

  4. FY 2000 report on the results of the regional consortium R and D project - Regional consortium R and D. Third year report. R and D of the transversal maintenance technology; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki consortium kenkyu kaihatsu. Odanteki maintenance gijutsu no kenkyu kaihatsu (dai 3 nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    For the purpose of eliminating bad effects of the maintenance/management of equipment/facilities of plants which are made separately and of heightening efficiency of work and improving technology, persons in charge of maintenance/management, persons requesting maintenance/management and official research institutes proceeded as one with the construction of a system doing maintenance/management work. Concretely, three parties cooperated in integration and enhancement of element technologies such as inspection/diagnosis and arrangement/repair and developed the maintenance/management system (maintenance center) which does not specify objects. Based on this system, development was made of the network connecting between the center and facilities, diagnosis car, and multi-functional repair car. Studies were made on the following three. As to the system development, conducted were the development of a deterioration mode prediction system, basic structuring of a remote maintenance network, and making of fatigue/corrosion/abrasion database. Concerning the tool development, technology to foresee damages on the static portion such as piping, etc. were developed. In the study of field application, conducted were analysis of maintenance flow, development of retrieval system of equipment parts/personnel/operation procedures and multi-functional repair car, etc. (NEDO)

  5. Fiscal 2000 regional consortium research and development project - regional new technology creation research and development. Practicalization of polymer back light (1st fiscal year); 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki shingijutsu soshutsu kenkyu kaihatsu bun'ya seika hokokusho. Polymer back light no jitsuyoka ni kansuru kenkyu (daiichi nendo)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Efforts are being exerted to develop technologies for fabricating polyvinyl carbazole thin film, 100 nm or less in average thickness and 400 mm square in area, for the construction of an exothermal sheet utilizing polymer semiconductor light emission diodes. Activities are conducted in the three fields of (1) the development of large area light emission sheets for use in polymer LEDs (light emission diodes), (2) the study of manufacturing technologies, and (3) the study of technologies for testing, analyzing, and evaluating the material. In field (1), a polyvinyl carbazole thin film is fabricated by the use of a low viscosity resin liquid application method, and a film fabrication technique is established for fabricating a thin film on a 400 mm square substrate within {+-}3 nm of film thickness error. In field (2), studies are conducted of an integrated large area thin film application/transportation system, application device mechanisms, and control sequences for application devices. Through studies of technical literature, exhibitions, and research institute meetings in Japan and overseas, it is concluded that the polymer back light is commercially feasible. (NEDO)

  6. Fiscal 2000 regional consortium research and development project - regional new technology creation research and development. Development of system-on-chip mobile IC power source (1st fiscal year); 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki shingijutsu soshutsu kenkyu kaihatsu seika hokokusho. System on chip mobile IC dengen no kaihatsu (daiichinendo)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Efforts are made to develop a switched capacitor (SC) integration technology based on the technology of switched capacitor power sources capable of efficiently performing voltage conversion without using magnetic parts such as coils. Activities are conducted in the six fields of (1) deciding on a programmable SC circuit, (2) circuit simulation, (3) layout design, (4) marketing and the contents and results of the studies, (5) profitability and the contents and results of the studies, and (6) a patent information survey. In field (1), a series fixed type DC-DC converter and a ring type converter are compared with each other in terms of electrical and theoretical characteristics, the number of devices to constitute the circuit, and ease of control, and the ring type converter is selected to serve as a general purpose SC power source. An efficiency simulation is conducted, as the result of which it is proved that the power source have the features that a practical power source should have. In field (2), for carrying out integration for the selected SC power source, a circuit simulation is conducted for the disclosure of IC power source constituent characteristics and dimensions are determined for the layout. (NEDO)

  7. FY 2000 report on the results of the regional consortium R and D project - Regional consortium energy R and D. Third year report. Ultra-high density information storage component; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki consortium energy kenkyu kaihatsu. Chokomitsudo joho storage component (dai 3 nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    For the purpose of industrially creating next generation HDD (hard disk drive), the development was proceeded with of ultra-high density and energy saving type next generation HDD system. The following four were conducted: 1) development of active type magnetic head arm for high speed writing/reading-out; 2) development of ultra-smooth low-noise medium using ultra-clean process; 3) establishment of component technology of high efficiency lubricating materials; 4) development of reproductive magnetic head using TMR device. TMR device is an ultra-high sensitivity magnetic detecting device composed of three layers of ferromagnetic material/insulator/ferromagnetic material. As to the development of regenerative magnetic head, studies were made of the reduction in resistance of TRM device, fabrication of submicron device and system integration. For the development of low resistance TMR device, thickness of Al layer, oxidation conditions and heat treatment conditions were studied in detail. The tunnel joining was obtained in which RA value and TMR ratio are 80 ohm(center dot){mu}m{sup 2} and 30%, respectively, in case of the thickness of Al layer of 6.6 (angstrom). (NEDO)

  8. Report of the results of the fiscal 1997 regional consortium R and D project. Regional consortium field / R and D on downsizing technology of machine tools (first fiscal year); 1997 nendo chiiki consortium kenkyu kaihatsu jigyo. Chiiki consortium energy bun`ya / kosaku kikai no downsizing gijutsu ni kansuru kenkyu kaihatsu (daiichi nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    The R and D were conducted aiming at downsizing machine tools by combining microfactory technology owned by Mechanical Engineering Laboratory, Agency of Industrial Science and Technology of MITI with fundamental element technology owned by industrial groups of machine tools. In fiscal 1997, as to the study of spindle drum indexing mechanism in making main spindle multiaxial, the following have been finished: the basic study through openness of controller and networks, confirmation of function of the equipment acquired, and initialization of the driver software. Relating to the study on machining conditions of dry machining processing, delivery and installation of cold air machining instrument have been finished. Concerning the optical measurement of spindle radial run-out vectors, a system to make optical no contact measurement of radial direction components of rotation error was constructed with high accuracy pin gauge attached to the work spindle clamp mechanism as a medium. About the study on sensor-based state recognition technology, specifications for various kinds of sensor were decided on. 5 refs., 67 figs., 3 tabs.

  9. FY 2000 report on the results of the regional consortium R and D project - Regional consortium R and D field. First year report. Development of an on-line/real-time dioxin measuring system; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki consortium kenkyu kaihatsu bun'ya. Dioxin no on line real time keisoku sochi no kaihatsu (dai 1 nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    With the supersonic molecular jet resonance multiple photon ionization mass analysis method (SSJ method) as basement technology, the development was proceeded with of a portable dioxin spectrometer to measure dioxins emitted from incinerator on line and at real time and to control/improve the state of combustion. Studies were made in the following three fields: 1) development of pico second wavelength variable color laser; 2) development of a flight hour type mass spectrometer; 3) development of the pretreatment technology suitable for analysis of dioxins, precursors, etc. by supersonic molecular jet spectroscopy. In 1), the prototype was fabricated to assess the performance, and it was confirmed that the prototype has the targeted characteristics. In 2), as to the coaxial ionization and ring double skimmer electrode system, the convergence and mass resolution of ion were measured, and a possibility of the commercialization was obtained. In 3), it was confirmed that there is a possibility in rapid pretreatment by alumina fiber filter. (NEDO)

  10. Report of the results of the fiscal 1997 regional consortium R and D project. Regional consortium field / Development of medical equipment use super devices using micro-3D processing technology (first fiscal year); 1997 nendo chiiki consortium kenkyu kaihatsu jigyo. Chiiki consortium bun`ya / micro sanjigen kako gijutsu ni yoru iryo kikiyo super device no kaihatsu (daiichi nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    The R and D are conducted of medical equipment use super devices using technical seeds of Mechanical Engineering Laboratory and micro-3D processing technology which was developed from precision processing technology, etc. which is integrated in the Suwa area, Nagano prefecture. For it, the following R and D are carried out: 1) development of micromechanism: DC3V driven micromotor, chemical use microtube with diameter of 0.1mm, micro-delivery mechanism of chemicals, misting mechanism of chemicals, microjoint, microconnector and cable, and microfilter. 2) development of microsensor: small size/non-contact, microflow of chemicals, and sensor to detect emission of bubble. 3) development of control system. In fiscal 1997, in 1), each element was trially made to clarify subjects. In 2), it was made clear that as flow sensor, ultrasonic type, electromagnetic type and thermal mass flow type are viable, and as bubble sensor, optical sensor is viable. In 3), the following were conducted: information collection of contactless charging circuit, and trial fabrication of liquid crystal polymer ultra thin casing. 9 refs., 84 figs., 30 tabs.

  11. Report of the results of the fiscal 1997 regional consortium R and D project. Regional consortium field / Development of 3D high speed bio-micromanipulation system (first fiscal year); 1997 nendo chiiki consortium kenkyu kaihatsu jigyo. Chiiki consortium bun`ya / sanjigen kosoku bio-micromanipulation system no kaihatsu (daiichi nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    This is aimed at developing a 3D high speed bio-micromanipulation system for small objects such as cells, bacteria and DNA. In fiscal 1997, a common platform for putting together and integrating individual technology owned by each member is trially fabricated, and the R and D of element technology to be integrated to it is divided up among members. As to selection/transportation technology, a basic experiment on screening by laser beams and electric field is conducted, and microfluid chips isolating bacteria are fabricated. Concerning positioning/fixation technology, cells and others which are comparatively larger than bacteria are freely positioned, and microdevices for fixation are trially fabricated. Relating to power measuring/controlling technology, developed is a multi-freedom degree contact type micromanipulator and a multi-axial minute sensor which enables the 3D mechanical manipulation of cells of the above-mentioned size, etc. The minute processing technology is developed to trially fabricate various microdevices which are integrated into the common flatform. 42 refs., 89 figs., 9 tabs.

  12. FY 2000 report on the results of the new regional consortium R and D project - Immediately effective type new regional consortium R and D. Study of the developmental technology for high-density recording materials using inverter plasma; 2000 nendo chiiki shinsei konsosiamu kenkyu kaihatsu jigyo - Sokkogata chiiki shinsei konsosiamu. Inbata purazuma wo mochiita komitsudo kiroku zairyo kaihatsu gijutsu no kenkyu kaihatsu seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For the purpose of reducing the price and size of large-capacity hard disk drive (HDD), using inverter plasma technology, developmental study was conducted of production technology of substrate for HDD using blue sheet glass as a main material and film formation technology in the media for HDD, and the FY 2000 results were reported. In the development of low-cost glass substrate, formation technology of the barrier layer to block the elution of alkali component was developed for blue sheet glass substrate. As to the development of formation technology of low-cost recording layer, developmental study was made of technology of formation of the layer as undercoat of magnetic recording layer onto the barrier layer. As a result of the experiment, the following were confirmed: metal chromium is well fit for the barrier layer and can be a proposed material of undercoat; it is possible to construct the magnetic media layer on the glass substrate. Concerning the development of the inverter plasma film formation unit, a unit was developed where it is possible to form the film of blue sheet glass substrate with a size of 3.5 inches. (NEDO)

  13. FY 2000 report on the results of the regional consortium R and D project. First year report. Regional new technology creation R and D. Development of the technology to produce useful substances by biosynthesis engineering; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki shingijutsu soshutsu kenkyu kaihatsu. Seigosei kogaku ni yoru yuyo busshitsu seisan gijutsu no kaihatsu (shonendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    On the basis of the basement technology such as the cloning technology of biosynthesis enzyme gene groups of secondary metabolite of plant and the identification technology of the expression protein enzyme function in the heterologous expression system, the development was proceeded with of the technology to produce useful substances using the biosynthesis function of plant components. Studies were made in the following four fields: 1) cloning of genes of useful biosynthetic plants and functional elucidation/application of the related genes; 2) analytical technology of genes and proteome; 3) development of plant genome database; 4) comprehensive investigational study. In FY 2000, in 1), the cloning was conducted of biosynthetic enzyme genes of oxidation resistant polyphenol, bioactive triterpenoid saponin, etc., and the function of a part of the enzyme proteins was elucidated. In 2), as the basic technology for improving producibility of secondary electrophoresis, fabricated was the electrophoresis chip so structured that slide glasses on which micro-channels were two-dimensionally fabricated were joined. (NEDO)

  14. FY 2000 report on the results of the regional consortium R and D project - Regional consortium field. Second year report. Development of the technology to combine plastic and metal using biodegradable natural polymer; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki consortium bun'ya. Seibunkaisei tennen plastic to kinzoku no fukugoka gijutsu no kaihatsu (dai 2 nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The development was proceeded with of the electromagnetic wave shielding technology composed of plastic body, chitosan containing biodegradable coating and electroless metal plating layer. Key technologies are for formation of biodegradable electroless plating use coating using chitosan as chemical adsorption carrier of Pd and for separation of the body and the metal thin film by the environment-harmony method for recovery of the material. Studies were made in the following 5 fields: 1) method to produce low molecular chitin and chitosan; 2) application of biodegradable materials to electromagnetic wave shielding; 3) evaluation of physical properties and function of a new electromagnetic wave shielding system; 4) R and D of the degradation method of the new electromagnetic wave shielding system; 5) comprehensive investigational study. In FY 2000, in 1), conditions for production of chitosan degrading enzymes were determined, and also the scale of bio-reactor was increased up to 20L. Further, chitosan was so re-synthesized that it disperses to the electroless plating use primer. (NEDO)

  15. Report of the results of the fiscal 1997 regional consortium R and D project. Regional consortium energy field / development of nano-structured materials for ceramic bearing application (first fiscal year); 1997 nendo chiiki consortium kenkyu kaihatsu jigyo. Chiiki consortium energy bun`ya / ceramic bearing yo nano seigyo zairyo no kenkyu kaihatsu (daiichi nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    For the purpose of developing high efficient ceramic bearing using nano-structured materials, technical development was proceeded with of raw material powder treatment, forming sintering, processing, structural analysis, property evaluation, etc. As to the study of manufacturing of ceramic balls, the following were conducted by the method developed at Osaka Prefectural Institute of Industrial Technology: coprecipitation laminate processing of ZrO2-Al2O3 system to alumina powder at Okumura Crucible Mfg. Co. Ltd., spherial press processing and sintering at Kyocera Co. Ltd., and precise machining at Nippon Pillow Block Mfg., Co. Ltd. The performance as bearing was measured of the ceramic balls obtained such as surface coarseness, sphericity, crush strength and fatigue life. Surface coarseness and sphericity were the same as those of bearing use silicon nitride, but crush strength was considerably low. In the experiment on rolling fatigue strength as bearing, separation occurred within 100 hours even at a load of 100kgf. It is thought that this is because of the pores remaining on the surface, and the measures to be taken for long life were studied. 12 refs., 64 figs., 27 tabs.

  16. Report of the results of the fiscal 1997 regional consortium R and D project. Regional consortium field / R and D on process adaptive type flexible robot technology (first fiscal year); 1997 nendo chiiki consortium kenkyu kaihatsu jigyo. Chiiki consortium bun`ya / kotei tekiogata flexible robot gijutsu ni kansuru kaihatsu (daiichi nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    For the purpose of establishing robot technology required for the construction of a highly functional automation line with flexibility, the R and D were conducted. At Kumamoto University, a model following type power control system was studied into which polynominal trajectory formation algorithm was integrated. Also studied was damping control of robot arms in a disturbance environment. The following were further carried out: experimental verification (HIRATA Corporation) of the application of power control and intelligent control to transportation equipment production line, experimental verification (SAKURAI SEIGI Co. Ltd.) of the application of power control and intelligent control to semiconductor testing equipment, R and D (OMRON Corporation) of high accuracy force sensing and positioning system, research (Kumamoto Technopolis Foundation) on flexible arms/hands suitable for cooperative control, sensor-based skill (Electrotechnical Laboratory), research (Kyushu National Industrial Research Institute) on tactual system and tactual sensing, etc. 20 refs., 86 figs., 14 tabs.

  17. FY 1999 Project of information exchange by researchers on environmental problems in the Asia-Pacific region. Report on investigations for establishing Environmental Technology Research Network in the Asia-Pacific Region; 1999 nendo Asia/Taiheiyo chiiki kankyo mondai kenkyusha koryu sokushin jigyo hokokusho. Asia/Taiheiyo chiiki kankyo gijutsu kenkyu kaihtsu network kochiku chosa

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    Described herein are the results of the FY 1999 activities for the project of information exchange by researchers on environmental problems in the Asia-Pacific region. In 1999, the 6th International Workshop was held in City of Yokkaichi, Mie Pref., with the theme (Implementation of international collaborative researches on environmental technology) at International Center for Environmental Technology Transfer, with a total of more than 50 domestic and foreign attendees actively participating in the discussion. The keynote addresses were (Use of coal and environmental improvements) presented by Mr. Yoshisada Nitta, Director at Central Research Institute of Electric Power Industry, and (Potential of clean fuel from biomass) presented by Dr. Kyu-Wan Lee of Korea Research Institute of Chemical Technology. The overseas surveys were conducted to promote the use and registration of the databases, and to identify problems by the on-the-spot investigations in Vietnam, the Philippines and Republic of Korea. At the same time, the mini-workshops were held for information exchange with the research institutes. It is keenly felt that Environmental Technology Research Network in the Asia-Pacific Region (ENTERNET-APR) works certainly in various areas, with the attendees in the last workshops serving as the key persons. (NEDO)

  18. FY 2000 report on the results of the regional consortium R and D project - Regional consortium energy R and D field. First year report. R and D of new composite polymer electrolyte for battery; 2000 nendo chiiki consortium kenkyu kaihatsu jigyo - chiiki consortium energy kenkyu kaihatsu bun'ya. Denchiyo shinki fukugo polymer kei denkaishitsu no kenkyu kaihatsu (dai 1 nendo) seika hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The development was proceeded with of new composite polymer electrolyte for Li secondary battery. The ultimate target of the development using this electrolyte is to get Li secondary battery markedly improved in safety/reliability which works at low temperature and controls thermal runaway. The composite polymer base electrolyte is composed of high molecular weight polyethylene oxide copolymer, ethylene oxide oligomer and Li salt, and it is finally cross-linked by heat or light. Studies were made in the following 6 fields: 1) R and D of the creation and optimization of new composite polymer electrolyte; 2) R and D of the commercialization of composite polymer electrolyte battery; 3) R and D on the safety of composite polymer electrolyte; 4) study of the synthesis of new electrolyte and catalytic activity of electrolyte-electrode interface; 5) R and D on polymer/oligomer composite electrolyte; and 6) comprehensive investigational research. (NEDO)

  19. FY 1999 report on the potential survey of the environmentally friendly type coal utilization system. Potential survey of the environmentally friendly type coal utilization system in the Philippines; 1999 nendo chosa hokokusho. Kankyo chowa gata sekitan riyo system kanosei chosa (Firipin ni okeru kankyo chowa gata system kanosei chosa)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    For the purpose of contributing to working out a master plan for the introduction of the environmentally friendly type coal utilization system in the Philippines, analysis was made of the state of coal use/the state of spread by industry mostly including the electric power industry, cement industry and residential/commercial use fuel sector. In the Philippines, the amount of coal use has rapidly been increasing since coal-fired power plants started operation. For the promotion of the use of domestic coals, it is important to reform low grade coal. Moreover, the technology is required which copes with the reinforced regulation against SOx emissions. In the cement industry, coal and coal ash have not yet been effectively used. Therefore, the following measures should be taken to increase the competitive force: energy conservation measures, reinforcement of environmental measures such as dust prevention, and effective use of the fly ash emitted from power plants. In general households and small-/medium-sized industries such as tobacco drying, they still use a large amount of charcoals and firewood, which is approximately 86% of the residential/commercial use energy. There is a growing tendency for the development/spread of coal briquette as substituting energy. (NEDO)

  20. Experiment list: SRX092303 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available trol description=This data represents a control being compared to experiments usi...ng antibody eGFP-GATA2 || control=Control_eGFP-GATA2 || control description=This data represents a control being

  1. Journal of Biosciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    However, the evolutionary history of the GATA family has not been completely understood. We constructed a complete phylogenetic tree with functional domain information of the GATA genes of both vertebrates and several invertebrates, and mapped the GATA genes onto relevant chromosomes. Conserved synteny was ...

  2. Fiscal 1999 geothermal energy development promotion survey. Report on environmental impact survey - 5 (Musadake area); 1999 nendo chinetsu kaihatsu sokushin chosa. Kankyo eikyo chosa hokokusho (No.B-5 Musadake chiiki)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-01-01

    For the purpose of clarifying the environmental impact to result from the digging of borehole N11-MD-3 in Shibetsu-gun, Hokkaido, a survey was conducted of changes in (1) hot spring water quality, (2) noise/vibration pollution, and (3) air pollution due to hydrogen sulfide, before and after the digging period. Under item (1), measurements were made at four hot springs, which involved weather, air temperature, water temperature, amount of welled-up water, pH, electric conductivity, etc. Although minor changes were observed during the measuring period, none was found related to the digging process. Under item (2), noise levels were measured during the drilling period along the site boundary, at a point 500m from N11-MD-3, the Kawakita hot spring, and the entrance of Sekiyunosawa. Along the site boundary, they were 55-60 dB during the drilling period, and there was an increase of 30dB attributable to the drilling. At the 500m point, there was a 15-20dB increase attributable to the same. Vibration levels during the drilling period were 40dB along the site boundary and less than the observable minimum at the other points. Under item (3), at four points along the site boundary (50m), hydrogen sulfide concentration in the air fluctuated by 0.01-0.21ppm when the steam discharge rate was 4.47-7.20t/h. (NEDO)

  3. Fiscal 1998 research report. Popularization project of the proper treatment technology of industrial wastes in Asian regions; 1998 nendo chosa hokokusho. Asia chiiki sangyo haikibutsu tekiseika shori gijutsu nado fukyu sokushin jigyo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-03-01

    Study was made on popularization of the proper treatment technology of industrial wastes in Asian regions. In principle, industrial wastes should be recycled in a country concerned, however, because of a difference in technology and industrial location, treatment of all industrial wastes in a country concerned is impossible. International cooperation is thus indispensable to promote proper efficient practical measures for environment. For studying the possibility of the international cooperation between Japan and Asian countries concerned for further recognition and solution of industrial waste problems, 'International Symposium on the Asian Industrial Waste Issue' have been held in Tokyo since 1997 for governmental staffs. In fiscal 1998, Malaysia was invited in addition to China, Philippines and Thailand for popularization of the proper treatment and recycling of industrial wastes in Asian regions. In every Asian countries, poor collection of discharge, treatment and disposal data is an obstacle to effective treatment. Technical cooperation and financial support by developed countries are demanded. (NEDO)

  4. Report for fiscal 1998 on industrial waste optimized treatment technologies dissemination and promotion business for asian region. Holding a symposium; 1998 nendo chosa hokokusho. Asia chiiki sangyo haikibutsu tekiseika shori gijutsu nado fukyu sokushin jigyo (symposium kaisai)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    An International Symposium on the Asian Industrial Waste Issue was held in Tokyo on 4 February 1999, where lectures were delivered by participants representing China, the Philippines, Thailand, Malaysia, and Japan. More 150 people attended the event, with questions and answered animatedly exchanged between the lecturers and the audience. The progress of urbanization is quite rapid in China, with the quantity of waste increasing every years to justify the anticipation that there will be a very sharp increase in refuse incineration in the near future. Raising a serious problem in China these days is the plastic waste. In the Philippines, there are no private-sector waste treatment/disposal facilities and corporations are not obliged to take care of their waste. Consequently, very little treatment or recycling is being undertaken. In Thailand, although waste treatment/disposal centers are in operation financed by the Government and private sectors, the cost is very high, and the Government policy is that private investment will be encouraged and supported in future. In Malaysia, privatization is in progress of the business of managing waste in the solidified form. A comprehensive waste treatment/disposal plant was completed in 1998 by the endeavors of a Denmark-Malaysia consortium, and is in operation since then. (NEDO)

  5. Survey of the measures to be concretely taken for CEV introduction/spread in the region; Chiiki ni okeru CEV donyu fukyu no tame no gutaiteki hosaku ni kansuru chosa

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For the Hanshin/Harima region where CEV introduction/spread are expected and there is a great pervasive effect, survey/study were conducted to plan the construction of facilities for natural gas vehicle fuel supply in future. In the survey, the paper summed up the state of CEV spread in the Hyogo prefecture, the state of construction of fuel supply facilities, etc. Further, by the questionnaire survey, the needs for construction of fuel supply facilities were grasped, and the intention of introducing natural gas vehicle was sounded out. In the study of a wide-area construction plan for natural gas fuel supply facilities, the Hanshin/Harima region was divided into zones based on geographical conditions, traffic system, etc., and the number of the needed fuel supply facilities was calculated which corresponds to the targeted number of natural gas vehicle introduction set up by the nation. Moreover, by the questionnaire survey, the needs for fuel supply facilities were grasped, and a method for setting up the order of priority in the construction was determined/proposed. Through the study of these, a manual was made out which is usable for the CEV introduction and the construction plan for natural gas fuel supply facilities in other regions. (NEDO)

  6. Research report for fiscal 1998. Basic research for promoting joint implementation, etc. (master plan for changing district heating boilers, Nizhniy Novgorod province); 1998 nendo chosa hokokusho. Nizhniy Novgorod shu chiiki danboyo boiler tenkan master plan

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    The above-named plan aims to reduce greenhouse gas emissions and enhance energy efficiency. The above-named Russian system for supplying heat and hot water is operated by the 51 local governments in the province, with boiler houses sited at 4047 locations. Almost all of them are approximately 30 years old, low in thermal efficiency because of their obsolete design and structure, costly because of their old parts and components wanting repair, and higher in fuel cost than those fired by natural gas. It is indispensable to replace the existing boilers with natural gas-fired boilers, and it is estimated that there are 4511 boilers that require such replacement. When the heat supply business operated by the local governments is modernized, the CO2 gas emission will be reduced by approximately 2.675-million tons/year. As for energy efficiency, it is found that replacement by heavy oil-fired boilers is the highest in terms of the effect of investment. (NEDO)

  7. Report on fiscal 2000 geothermal development promotion survey. No. C-5 Appi district environmental impact survey (1st phase); 2000 nendo chinetsu kaihatsu sokushin chosa hokokusho - No.C-5. 1. Appi chiiki kankyo eikyo chosa

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-08-01

    For the survey of weather in the Appi district, Iwate Prefecture, observation data covering the last decade were collected at weather stations and the like, and characteristics of weather, climate, and natural earthquakes were learned. In the survey of fluctuation in the spa water level, studies were made about fluctuation in water temperature, spring water, and river water, and basic data were collected for the examination of the impact to be generated by geothermal exploration well boring and short-term discharge tests. In the survey of animal and vegetables, the habitation and distribution of animals and vegetables at sites planned for geothermal well boring were investigated, and data were collected for the evaluation of the impact to be imposed on them by well boring or the like. In the survey dealing with nature conservation and landscape, laws and regulations governing nature conservation were collected and put in an easy-to-use order, and seasonal changes in the natural landscape were photographed and recorded. In the survey of noise/vibration and ground fluctuation, the background values were measured prior to the implementation of geothermal well boring, discharge tests, and so forth. (NEDO)

  8. Fiscal 1999 geothermal development promotion survey. Development potentiality survey - Strategic survey/nation-wide survey (Report on districts in Fukushima Prefecture); 1999 nendo chinetsu kaihatsu sokushin chosa. Kaihatsu kanosei chosa - senryakuteki chosa zenkoku chosa (Fukushimaken chiiki hokokusho)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-08-01

    For the effective implementation of the survey, data in existence were reanalyzed for the Bandai-Azuma district and Minami-Aizu/Nasu district, and a wide area geothermal model was built. Using the model, exploitable resources were assessed and a prioritized survey introduction program was worked out for the promotion of geothermal development. In compliance with the strategic survey implementation manual, the Bandai-Azuma district was assessed, and the northeastern-eastern foot area of Azuma Volcano and the northern foot area of Adatara Volcano were extracted as areas promising geothermal energy development. The Monte Carlo method was applied to the prioritized survey area at the northeastern foot of Azuma Volcano, and then it was estimated that, with a probability of not less than 90%, the exploitable resources would be 6.4MWe in the case of data accuracy of 0.5 or more and 12.0MWe in the case of data accuracy of 0.4 or more. Selected as promising areas in the Minami-Aizu/Nasu district were the Yanaizu-Nishiyama area, the neighborhood of Nasudake-Chausudake, and the neighborhood of Shiobara-Fuji. (NEDO)

  9. Fiscal 1999 achievement report on the venture business assisting type regional consortium - Minor business creation base type. Development of sodium hypochlorite activating system; 1999 nendo venture kigyo shiengata chiiki consortium kenkyu kaihatsu seika hokokusho. Jiaensosan natrium kasseika sochi no kaihatsu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The aim is to develop and widely diffuse a cleaning, sterilizing, and disinfecting system (chemicals and device) which is nearer to the best. A safety-conscious diluting/mixing technology was developed by which acid and sodium hypochlorite were mixed without generating any noxious gas. Using this technology, a mixing tube was developed, capable of homogeneously diluting chemicals in raw water and mixing them homogeneously. How the dilution and mixing occurred in the mixing tube was measured by an electric conductivity meter for evaluation, data on the state of diluting of the solution were plotted in a graphic chart for study, a comprehensive deliberation was made of performance, cost, etc., and then a device was experimentally constructed. A concentration level detecting sensor section and a metering pump were developed. The pH adjustment and disinfecting effect were assessed using various acids, and then it was found that the acetic acid was the most suitable in view of safeness, disinfecting effect, stability, and ease of adjustment. Based on the result of a comprehensive technical survey, a system was designed suitable for artificial dialysis, food processing, and cleaning and disinfecting of endoscopes. A prototype was built, and the development effort progressed to reach a level where a prototype was built and subjected to a field test. (NEDO)

  10. Report on the project for working out the new energy vision of the Kamoto Town area. Feasibility study; 2001 nendo Kamoto machi chiiki shin energy vision sakutei tou jigyo fijibiriti hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For the realization of 'the introduction of biomass of animal manure/garbage,' an important item in 'the new energy vision of the Kamoto Town area (Kumamoto Prefecture),' a feasibility study was conducted. For the resource retrieval of animal manure, etc., only composting is generally conducted. In this project, however, two projects are combinedly conducted: project for producing biogas (main component: methane gas) and liquid fertilizer and project for producing compost. The project pursues the reduction in running cost by self-sufficiency of energy and the environmental preservation by recovery of methane gas. The plant for producing biogas and liquid fertilizer consists of garbage crusher, storage tank, anaerobic fermentation tank and generator. The plant for producing compost consists of solid liquid separator, fermentation tank, agitator and smell remover. In the feasibility study, the following were carried out: study of the establishment of specifications/selection of the necessary conditions for a plant tentatively named the Kamoto Town Biomass Center, study of the proposed site for plant, survey of the financial plan, survey of the operational system, study of the project promotion system, etc. (NEDO)

  11. Fiscal 1999 survey report. Survey of environmental effect pertaining to survey for geothermal development and promotion (Hakusuigoe area); 1999 nendo chinetsu kaihatsu sokushin chosa no uchi kankyo eikyo chosa (Hakusuigoe chiiki) hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    This survey of environmental effect was intended to grasp actual state concerning some environmental factors that should agree with survey/development and also to assess its effect on the surrounding environment, particularly as a part of the 'stage C' in the 'survey for geothermal development and promotion', which was implemented by the Government for the purpose of guiding geothermal development by enterprises. This report compiles the plan details of the environmental effect survey to be conducted in the Hakusuigoe district, and presents proposals. The survey area is in the north of Makizono-cho, Aira-gun, in Kagoshima prefecture and situated about 3 km west of Onaminoike in Karakunidake, one of the peaks in the Kirishima mountain range. The Ogiri geothermal power plant is in the west of the site, a tourist spot Ebino Heights about 4 km northeast, and the Kirishima hot-spring about 4 km southeast. The location is in the national park, with a part designated as No. 2 and 3 class special areas. The environmental effect survey was divided broadly into such categories as fauna/flora, hot-spring water alteration and inland water, landscape, noise/vibration/subsoil, and air/water, and was further subdivided in accordance with the actual operations. (NEDO)

  12. General evaluation survey report on fiscal 1998 investigation of geothermal development and promotion. No.C-3 Akinomiya area (Third); 1998 nendo chinetsu kaihatsu sokushin chosa sogo hyoka chosa hokokusho. No.C-3. Akinomiya chiiki (dai 3 ji)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-01-01

    With Akinomiya area (11.4 km{sup 2}) selected in Akita prefecture as the survey C area in fiscal 1996, the first survey was conducted. This paper is a summary of memorandum from an advisory standpoint on the evaluation survey concerning the fiscal 1998 investigation results. The contents of the survey are geological investigation, precision gravity exploration, soil gas investigation, electromagnetic exploration, fluid chemical investigation, well drilling and short-term eruption test. An alteration zone was recognized around the place where clay minerals used to be mined, with the center of the zone situated 500-600 m east of a fault. The alteration condition of Akinomiya area seems to be different from that of Doroyu Wasabisawa area. The supplementary gravity survey was carried out at total 58 points in fiscal 1998. It is desirable to set supplementary measuring points over a wide surrounding area for a depth structure analysis. Also performed was a study of mercury concentration in the soil gas. While CSAMT and TDEM methods were employed for the electromagnetic exploration, a suitable method should be carefully selected for a wide area high-density exploration. Consistency with adjacent areas is essential in preparing investigation items particularly for a geothermal model. (NEDO)

  13. Protect and bring up forest of Kuse Town. New energy vision at Kuse Town area; 2001 nendo Kuse cho chiiki shin energy vision. Kuse no mori to machi wo mamori sodateru

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    With an objective of accelerating introduction of and uplifting consciousness on new energies at Kuse Town in Okayama Prefecture, investigations and discussions were given on the current situation of energy demand, quantity of new energies available, and the idea of introducing new energies, thus a new energy vision was established. The estimated amount of energy consumption in Kuse Town is 304,768 MWh/year, and the amount of carbon dioxide discharge associated therewith is 22,300 t-C. Regarding the energy consumption by departments, the industrial department accounts for 40%, followed by the transportation department accounting for 30%. The basic new energy introduction ideas have discussed town resident enlightenment and town resident participation projects, bio-mass energy utilization, such as bark combustion plant, procurement of wood resources remaining in forest, ligneous pellet and transportation fuel manufacture, utilization of edible oil into diesel fuel, introduction of photovoltaic power generation at public facilities and places of refuge, introduction of solar heat utilization system into public facilities and homes, large size wind power generation project, introduction of small size wind power generation into road illumination and places of refuge at disaster, small hydro-energy utilization system, and introduction of clean energy fueled automobiles and fuel cells. (NEDO)

  14. FY 1998 report on the comprehensive analysis in the geothermal development promotion survey. Tertiary. No.C-3 Akinomiya area; 1998 nendo chinetsu kaihatsu sokushin chosa sogo kaiseki hokokusho. No.C-3 Akinomiya chiiki (Dai 3 ji)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-01-01

    The geothermal development promotion survey was conducted in the Akinomiya area, Ogachi county, Akita prefecture, and the FY 1998 results were summed up. In the surface survey, geology/electromagnetism/precision gravity survey was made as supplementary survey, and re-analysis of 3D models was made. In the well survey, three wells of N10-AY-6 to N10-AY-8 were drilled to conduct the core examination, temperature/pressure logging, sampler logging, short-term jetting test, etc. Also conducted were the reservoir evaluation, environmental effect survey, etc. by pressure monitoring and water level observation. The results of the comprehensive analysis predict the following. In the Akinomiya area, there exists heat source in deep parts around Mt. Yamabushi-dake and Mt. Takamatsu-dake; Being given heat from this heat source, rocks, ground water and volcanic gas react on each other to form geothermal fluids. It is presumed that high temperature geothermal reservoirs exist in the east of the area. Moreover, the pressure distribution indicates that the Akinomiya reservoir and Wasabizawa reservoir can be a chain hydraulically. On the other hand, the west of the area is the low temperature and heat conductive area, and therefore, there seem to be no high temperature fluids there. (NEDO)

  15. Fiscal 2000 survey report. On-site survey of local state of affairs such as energy efficiency enhancement (Mongolia); 2000 nendo energy shohi koritsuka nado chiiki josei genchi chosa hokokusho. Mongoru

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    Information was collected and surveys were conducted in Mongolia in an effort to acquire information necessary for energy related technical cooperation. The results were arranged in four fields which are (1) the current state of economy and the future trend, (2) current state of energy production/consumption and the future trend, (3) current state of power supply/demand and power facilities and the future trend, and (4) the current state of energy efficiency enhancement and the future trend. Mongolia has a population of 2.45 million at a density of 1.57/km{sup 2}, with Ulan Bator the capital inhabited by 0.69-million people. The mean air temperature is -10 degrees C in the period November through March, which makes heating indispensable. Power stations are of the heat and power cogeneration type. Power is supplied by the Eastern, Central, and Western systems, with the Central system producing 80% or more and independent power sources 10%. Its economy has already recovered from the confusion that followed the change of its national constitution. Mongolia's demand for power lingers low, however, and its facilities can produce more electricity than what is now wanted. Energy efficiency enhancing activities should start with the upgrading, reinforcement, and maintenance of the existing facilities and with independent power sources. (NEDO)

  16. Report on the initial-stage survey of the new energy vision of the Izumi city area; 2001 nendo Izumi shi chiiki shin energy vision shoki dankai chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Izumi City, Osaka Prefecture, an investigational study was conducted of the structure of energy consumption, existence amount of new energy, subjects in utilization of new energy, etc. The energy consumption amount of the city is increasing mostly in electric power in the residential sector, but is decreasing in the business sector and industrial sector. It is leveling off in the transportation sector, but only the consumption amount of gasoline is markedly increasing. In the potential amount of new energy utilization in the city, the rate of solar energy/clean energy vehicles was high. As a result of the study, the following were made clear: It is effective to introduce new energy mostly used for citizen such as the household use photovoltaic power system/solar heat use hot water supplier/hybrid private car. Of waste energy, wind energy, biomass energy and temperature difference energy, it is effective for city to take the initiative in introducing them. It is effective for city/proprietor to introduce the natural gas cogeneration. (NEDO)

  17. Museum of water, green and the sun - Marumori. New energy vision of the Marumori Town area; 2001 nendo Marumori machi shin energy vision hokokusho. Chiiki shin energy vision sakutei tou jigyo (Mizu to midori nto taiyo no hakubutsukan Marumori)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    In Marumori Town, Miyagi Prefecture, a 'new energy vision of the area' was worked out which indicated the assessment of the energy reserved in the town and the course of new energy to be determined in the future. The energy consumption amount of Marumori Town in FY 1999 was estimated at 47,535 kL in crude oil conversion. By sector, the energy consumption amount was the largest in the order of the industrial sector, the transportation sector and the commercial/residential sector. The availability of new energy in Maruyama Town was estimated at approximately 480,585 GJ, of which the solar energy utilization was the largest, 53.1%. The photovoltaic power generation was the next largest, 25.4%, followed by the temperature difference energy (underground water), 8.9%, clean energy car, 7.8%, etc. As a plan for the first introduction of new energy, the following were studied: introduction of photovoltaic power generation facilities to the Marumori Town Office, introduction of new energy to public facilities around the Fudoson Park, introduction of wind power generation facilities to the town-run pasture, construction of the Marumori-type biogas plant, construction of a mini hydroelectric power plant, introduction of clean energy car to public vehicles, introduction of new energy facilities to the area of Kawa-no-Eki (station of the river), etc. (NEDO)

  18. Stress estimation around the survey wells in Hanshin-Awaji area by means of AE/DR and DSCA experiments; AE/DR ho to DSCA ho ni yoru Hanshin Awaji chiiki chosa kui shuhen no chikaku oryoku sokutei

    Energy Technology Data Exchange (ETDEWEB)

    Ito, H; Kuwahara, Y; Nishizawa, O [Geological Survey of Japan, Tsukuba (Japan); Yamamoto, K [Tohoku University, Sendai (Japan). Faculty of Science; Sano, O [Yamaguchi University, Yamaguchi (Japan). Faculty of Engineering; Yokoyama, T; Kudo, R [OYO Corp., Tokyo (Japan); Xue, Z [Kiso-Jiban Consultants Co. Ltd., Tokyo (Japan)

    1996-05-01

    A total of 5 wells were excavated in the Hanshin-Awaji area (Ikeda, Takarazuka, Tarumi, Hirabayashi and Ikunami) to collect the core samples, which were analyzed by the AE/DR and DSCA methods to determine crustal stresses. For the AE/DR analysis, the core sample was cut in the vertical direction, and in the horizontal direction at intervals of 45{degree}. The sample of unknown orientation was provided with a datum line common for both methods, to compare the results by these methods. A load was applied to the sample, provided with an AE sensor and strain gauge on the sides, in the longitudinal direction. For the DSCA analysis, the core sample was cut into a cube having a side length of 33mm, with a pair of planes directed in parallel to the datum line. A total of 18 strain gauges, 10mm in gauge length, were attached to the cube. The AE/DR analysis gave the maximum and minimum principal stresses in the horizontal plane and stresses in the vertical direction, whereas the DSCA the maximum, intermediate and minimum principal stresses. 3 refs., 7 figs.

  19. Processing of the reflection seismic data which were acquired in the continental shelf off the north-west Australia; Australia hokusei tairikudana chiiki ni oite shutokusareta hanshaho chishitsu tansa data no shori ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    Amano, H [Japan National Oil Corp., Tokyo (Japan)

    1996-05-01

    Reflection exploration of earthquakes was conducted in the continental shelf off the north-west Australia, and a report is made concerning the processing of the acquired data. A GI gun was used as the vibration source, and a wave quite similar to a pulse was generated. Hydrophones were arranged with group intervals of 12.5m, and high-resolution field data were acquired. An analog low cut filter was used to suppress the large-amplitude swell noise attributable to sea conditions. A digital filter was designed for the purpose of eliminating coherent noises from other survey ships. At the ultimate stage the f-k filter was applied to each of the shot records, and the effectiveness was verified. The traverse line was as long as 6700km and therefore the design window was set relatively long at 2.0-4.0s in the residual static correction phase. It was found that this setup is effective in improving the continuity of the reflection surface. 5 figs., 1 tab.

  20. Time domain electromagnetic method for petroleum exploration. Part 3. Case study in Yoneyama area; Sekiyu tansa ni okeru TDEM ho data shutoku to kaiseki ni tsuite. 3. Niigataken Yoneyama chiiki deno tekiyorei

    Energy Technology Data Exchange (ETDEWEB)

    Ishikawa, H; Mitsuhata, Y [Japan National Oil Corp., Tokyo (Japan); Shiga, N [Mitsui Mineral Development Engineering Co. Ltd., Tokyo (Japan)

    1996-05-01

    The result is reported of a TDEM method-based survey for oil exploration conducted in the Yoneyama district, Niigata Prefecture. The survey covered the southern part of the sedimentary basin in the prefecture, known for the poor quality of the seismic prospecting data that had been recorded. The purpose of the TDEM method-assisted survey was to probe the high-resistivity volcanic rock structure and low-resistivity sedimentary layer, and to know their details. The obtained data were subjected to interpretation by use of the 1D inversion method. A 4000m-deep level was subjected to interpretation, 34 data were inputted, and the early model consisted of 20 layers. As the result, it was found that there was a high-resistivity layer ranging from the ground surface down to a depth of approximately 500m, that a low-resistivity layer 2500-3000m in thickness lay thereunder, and that the lowest layer had a high resistivity of approximately 100 Ohm m. By comparing these data with the geological structure, structures were disclosed corresponding to the rock bed depth, Ukawa syncline, Ishiguro anticline, and Yoneyama mountain mass. 3 refs., 10 figs., 1 tab.

  1. Effects of air pollution on the health of regional inhabitants. Part I. Survey of disease state by Enquete. Taiki osen ga chiiki jumin no kenko ni oyobosu eikyo ni tsuite-dai ippo; anketo ni yoru shojo chosa

    Energy Technology Data Exchange (ETDEWEB)

    1973-01-01

    From May to July 1972, the disease states of 2096 inhabitants (710 men and 1386 women) in the Fukushima Ward of Osaka City, where the concentration of sulfur trioxide in the air was (yearly average) 1.81 mg/day/100 sq cm and that of carbon monoxide was 5.3 ppM, and that of nitrogen dioxide was 5.3 ppM, were surveyed. The number of persons surveyed was 2.9% of all the inhabitants of the Ward. Twenty-two point one percent were in their thirties, 20.1% in their forties, 16.5% in their sixties, and 15.3% in their fifties. Those who experienced chest diseases were 2.9% of the total, and those with heart diseases were 1.5%. Non-smoking men were 31.7% of the total. The percentage of long-term complaints of cough and sputum was 11.8% in the eastern section, 11.2% in the northern section, 10.0% in the southern section, 6.8% in the western section of the Ward. The frequency of complaints was highest in the sixties (12.7%), followed by 11.5% in the fifties, 11.3% in the thirties, and 8.6% in the forties. The frequency of complaints from men was 11.1% and that from women was 6%. The frequency of complaints was 19.8% in smokers, 8.5% in those who smoked less, and 6.8% in non-smokers. The frequency of complaints classified by residential area showed 12.6% in industrial areas, and 7.8% in residential and commercial areas. The frequency of complaints of inhabitants who live along National Road No. 2 was relatively high, ranging from 15.0 to 18.8%, and the frequency of complaints of inhabitants was apparently higher than those of people who only work in the Ward.

  2. FY 2000 report on the results of the data processing in the geothermal development promotion survey. Tertiary. No.B-7 Kuwanosawa area; 2000 nendo chinetsu kaihatsu sokushin chosa data shori hokokusho. No. B-7 Kuwanosawa chiiki (Dai 3 ji)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-01-01

    The comprehensive analysis was conducted of various data obtained in the geothermal development promotion survey conducted in the Kuwanosawa area, Yuzawa city, Akita prefecture, from FY 1998 to FY 2000. The geology of the Kuwanosawa area consists of the Pretertiary system and Quarternary system, through which intrusive rocks are recognized. Basement rocks are composed of the Paleozoic-origin crystalline schist and the Cretaceous-period granites which intruded into the schist. In the Kuwanosawa area, there were recognized no clear geothermal signs such as the discharge of geothermal fluids like hot spring, fumarolic gas, etc., places of high temperature and new geothermal alteration zones. The geothermal water of borehole N11-KN-1 is a low temperature/low CL concentration geothermal water which was stored in basement rocks, which is supposed to be the one conductively heated in the process of the meteoric water penetrating deep-underground. The geothermal system heat source in the Kuwanosawa area and the periphery is regarded as the relic magma which spewed out the volcanic rocks of Mt. Takamatsu-dake in the Quaternary period. However, the geothermal fluid included no components originating in high temperature volcanic emissions, and therefore, it is considered that the geothermal fluid was formed by the meteoric water conductively heated by volcanic heat source. (NEDO)

  3. Report on the site surveys in fiscal 1999 on the regional situations such as energy consumption efficiency improvement. The north-western part of China; 1999 nendo energy shohi koritsuka nado chiiki josei genchi chosa hokokusho. Chugoku seihokubu

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-03-01

    This paper reports the site surveys in fiscal 1999 on the energy consumption efficiency improvement in the north-western part of China. Although blessed with affluent natural resources, the regional development is delayed because of geographical restrictions and harsh climate conditions. Power generation facilities include hydroelectric power plants with output of 7.81 million kW, thermal power plants with 13.89 million kW, and power wind power plants with 70 thousand kW. The demand is estimated to increase by 4.6% annually in average. Thermal power plants are those made by the former Soviet Union, having poor power generation efficiency, which is scheduled to be improved by facility modification. Automation is also behind the times. These situations have brought about low reliability, delay in building electric power network in agricultural districts, and transmission wire loss (reducing 8.1% loss to 6% is planned). The electric power charge systems also have various problems. Environmental measures are also an issue. Dissolution of energy shortage as a result of developing the north-western area can be said a key to the future development of China. Xinjiang Electric Power Company is considering wind power generation as the center of new energy utilization. Representative facilities utilize affluent wind power available in the topography of the Tenzan mountain range in the suburb of Urumqi, the facilities being made of Denmark. Photovoltaic power generation is exiguous in realizability for the time being in terms of cost. Expectation is placed on fuel cells. (NEDO)

  4. Investigation of geothermal development and promotion for fiscal 1997. Investigation report on geological/alteration zone (No. B-5 Musadake area); 1997 nendo chinetsu kaihatsu sokushin chosa. Chishitsu henshitsutai chosa hokokusho (No.B-5 Musadake chiiki)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    This investigation was intended to clarify the possibility of existence of geothermal reservoir layer by elucidating the underground structure and thermal source forming the geothermal system around Musadake, in the area extending over Nakashibetu-cho and Shibetu-cho, Shibetu district, Hokkaido, and further by grasping the distribution and properties of the geothermal alteration zone. The geothermal system of the subject area seems to be governed by Musadake - Mt. Shitabanupuri fault, its incidental bent zone, and the graben extending to the southeast side. The thermal source is presumably a magma reservoir that evolved Musa volcano. The depth hot water is presumed to exist in the underground depth around Mt. Imora; and it seems that the thermal conduction and volcanic ejecta are caused by a chain of volcano-related magma reservoirs around Musadake, the thermal source presumably. The depth hot water is estimated to be not less than 250 degrees C and a high Cl concentration. Within the graben, mudstones are distributed such as Kawakita layer and Yokoushigawa geological layer. Moreover, it is possible that an impermeable layer due to alteration is spread. (NEDO)

  5. FY 1999 report on the geothermal development promotion survey data processing. No. B-7 Kuwanosawa area (secondary); 1999 nendo chinetsu kaihatsu sokushin chosa data shori hokokusho. No.B-7 Kuwanosawa chiiki (dai niji)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-10-01

    As a part of the FY 1999 geothermal development promotion survey - Kuwanosawa area, the following were conducted and the results were arranged: ground surface survey (electromagnetic survey, analysis of numerical map), N11-KN-1 structure test boring survey, geothermal water survey, environmental effect survey, supplementary survey related to data processing, and comprehensive analysis. As to the reservoir structure, it seems that the area is low in water permeability from the results that there was seen little lost circulation at the time of drilling N11-KN-1 and that transmission coefficients in the water poring test indicated smaller values than those of the production well. In the underground temperature distribution, the temperature at a depth of 1,800m was 164.5 degrees C, which is lower than that in the neighboring Wasabizawa area. The geothermal water of N11-KN-1 does not have many dissolved constituents, showing alkalescence, which belongs to the Na-HCO{sub 3} type. As to the geothermal water of N11-KN-1, it seems that the meteoric water went down along faults and fractures, was stored in basement rocks and heated by heat conduction to form the geothermal water. In the Kuwanosawa area, there are seen very few geothermal signs on the ground surface, and a possibility seems to be small that high temperature and dominated geothermal fluids are active on a large scale. (NEDO)

  6. Fiscal 1999 geothermal development promotion survey. Structural boring survey work and analysis report (No.B-6 - Tsujinodake area); 1999 nendo chinetsu kaihatsu sokushin chosa. Kozo shisui chosa koji oyobi kaiseki hokokusho (No.B-6 Tsujinodake chiiki)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-12-01

    Cuttings from bores N11-TD-2 and N11-TD-3 drilled for the purpose of confirming the existence of geothermal resources in the Tsujinodake area were examined and tested, and the results of temperature logging, electrical logging, and water injection tests carried out during the structural boring work were analyzed. Activities were conducted in the four fields of (1) test boring work, (2) survey and measurement, (3) core cutting examination, and (4) comprehensive analysis. In bore N11-TD-2 positioned in the northern part of Tsujinodake, a sharp temperature rise was detected in the clay converted alteration zone between 200m above sea level and 200m below sea level. A high temperature of 215 degrees C was recorded at 200m below sea level, and it was found that high temperature geothermal water was in reserve at the bottom of the clay converted alteration zone between 100m above sea level and 200m below sea level. In bore N11-TD-3 in the southern wall of the Ata caldera, a convection type low temperature profile was observed at a shallow level and low temperature convection of underground water was detected there. (NEDO)

  7. FY 1998 geothermal development promotion survey. Report on the environmental effect survey (animals/plants, No. B-7 Kuwanosawa area); 1998 nendo chinetsu kaihatsu sokushin chosa. Kankyo eikyo chosa hokokusho (doshokubutsu, No.B-7 Kuwanosawa chiiki)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    This survey was conducted to estimate effects of drilling of geothermal exploration well on the environment, aiming at grasping the present state of environmental elements before the survey/development. As a result of the literature survey, the following distribution were confirmed in the fauna: 5 orders 10 families 19 species in the mammalia, 10 orders 25 families 73 species in the aves, 1 order 3 families 6 species in the reptilia, 2 orders 6 families 9 species in the amphibia, and 17 orders 179 families 719 species in the insecta. In the flora, a distribution of 132 families 670 species was confirmed. The results of studying the above indicated that in the fauna, there were 10 species such as antelope as valuable animal in the area surveyed and that it is necessary to pay much attention to the environmental preservation of the habitat for those animals in the well drilling associated with geothermal survey. In the flora, the 13 valuable animals selected as animal having a fear of extinction in the 'plant-version red list' were confirmed in the area surveyed and the periphery. Further, as to the plant colony, there are no important colonies in terms of preservation. In well drilling, important things are efforts exerted to restore to the original state of the area altered, prevention of the washed-away of mud water, etc., and efforts exerted to preserve the environment of vegetation. (NEDO)

  8. Survey report of FY 1996 on the questionnaires related to new energies for local governments in Kinki district; 1996 nendo chosa hokokusho (shin energy ni kansuru Kinki chiiki jichitai anketo chosa)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-03-01

    The photovoltaic (PV) power generation system having a lot of merits for environment and energy has been put into a diffusion stage from the viewpoint of its technology due to its progressive technology innovation. However, it is not widely diffused in the whole society due to its high cost. Systematic investigation for the future PV power generation was conducted through the verification of factors obstructing the diffusion promotion and the investigation of the problems. Then, questionnaires were conducted for local governments in the Kinki district. Proportion of the local governments positively promoting the introduction of PV power generation was 3.4%. Among new energies, 45.7% of the governments were interested in solar heat utilization, and 43.1% were interested in PV power generation. Proportion of the local governments understanding the subsidy system of MITI was 57.7%. For the explanation meeting of new energy introduction promotion, 55.4% of the local governments wanted to participate. Among the interesting themes, 61.0% were interested in the introduction examples, 52.4% were in the introduction support, 52.1% were in the introduction of fundamental knowledge, and 30.0% were in the latest technology trends and in the guide for introduction

  9. Report on a survey in fiscal 1998 for petroleum substituting energy development and utilization in the Pacific Ocean region; 1998 nendo Taiheiyo chiiki sekiyu daitai energy kaihatsu riyo chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    A survey was carried out to make available the information base that can collect, put into order and provide quickly and accurately the energy related information based on global environment problems in the Pacific Ocean region. Fiscal 1998 has performed a substituting energy demand and supply analysis in the Pacific Ocean region by adding the 1996 data, in addition to collection of the normal energy data. For the purpose of analyzing the petroleum substituting energy demand and supply in the Pacific Ocean region, data were collected from 17 countries for fiscal 1997 and the quarterly data for 1998. Retroactive data collection was also continued. The growth rate of primary energy supply in the Pacific Ocean region is 1.8% in average in the 1980's, and 3.8% in the 1990's. In contrast, the growth rate of petroleum substituting energies is 6.4% in the 80's and 6.2% in the former half of the 90's, being far higher than the growth rate of the primary energy supply. The share of the petroleum substituting energies accounting for in the primary energy supply has increased from 6.7% in 1980 to 12.1% in 1996. As the breakdown, nuclear power accounts for overwhelmingly large, followed by geothermal energy and new energies. (NEDO)

  10. FY 1999 geothermal development promotion study. Report on results of the elastic wave exploitation (reflection method) in No.B-5, Busadake area; 1998 nendo chinetsu kaihatsu sokushin chosa. Danseiha tansa (hanshaho) hokokusho No.B-5 Busadake chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    Described herein are results of the elastic wave exploitation (reflection method) conducted in the Busadake area in Hokkaido to elucidate the geological and geothermal structures. The elastic wave exploitation used large-size vibrators, an explosive and 4 courses of traverse (total length: 25.675 km, number of vibration-receiving points: 1,031, and number of vibration source points: 342). The data were processed by the standard procedure of the common reflection synthesis method. The acoustic velocity logging data, collected at the structural drillings N10-MD-1 and N10-MD-2, were synthesized to have the reflection seismic records. The existing surface geology, drilling geology and gravity data were compared with the recorded deep sections for the comprehensive discussion, to extract the reflection horizon corresponding to the geological boundary, and investigate the relationships of the discrete lines of deposit structures, unconformity relations and reflection with geological and default structures determined by the data analysis. The study results of the geothermal structures suggest possible formation of the geothermal reservoir deep in the west of the default found by Line-1, based on the observed temperature structures at the drilling N7-SB-1. (NEDO)

  11. Report on the FY 1998 geological structure survey. North Hay Gulch area, State of Colorado, the U.S.; 1998 nendo kaigaitan kaihatsu kanosei chosa hojo jigyo chishitsu kozo chosa hokokusho. Beikoku Colorado shu North Hay Gulch chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-02-01

    Test boring at 21 places and core sampling were conducted during September-November 1998 along the ridge to the west of Hay Gulch and in the direction crossing the northwest of the drainage canal and National King Coal Mine (NKC coal mine). It was found that A layer which NKC coal mine is now drilling is the upper part of A coal seam where the seam bifurcates. The boundary to the west of the thick A coal seam is not clear, and there is a possibility that more reserve from the thick coal seam and single seam exists toward Cherry Creek. In the existing coal drilling area, the lower A seam existing under the upper A seam becomes thin rapidly, and the seam thickness becomes less than 1 foot. The average thickness of thick coal seams is 9.9 feet, and the area is 780 acres along the ridge. The upper A coal seam except the above is 5.5 feet in average seam thickness and 910 acres in area. The reserve is 23 million tons. As to the quality of thick coal seam, the average heating value is 7,089 kcal/kg, sulfur content 0.68%, and ash content 7.50%. As coal drilling conditions, the same level as or higher than those of NKC coal mine is expected. (NEDO)

  12. Regional new energy vision for Kamaishi City. Energy to create community's future out of natural resources; 2001 nendo Kamaishi shi chiiki shin energy vision. Megumi kara machi no mirai wo tsukuru energy

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    For promoting the introduction of new energy and for enhancing people's consciousness of such at Kamaishi City, Iwate Prefecture, surveys and studies were conducted involving the amount of energy needed by the city, the amount of new energy resources in existence, and new energy introduction projects, and a vision was formulated. The city consumed 5,334 times 10{sup 12}J in fiscal 1999, which amount occupied 0.034% of the nation-wide consumption. Concerning the total consumed by the city, the manufacturing sector was responsible for 36% and the transportation sector for 24%. Oil provided 60% of the energy, and electric power 24%. These percentages were similar to those found across the nation. The amount of carbon dioxide emissions was estimated to be equivalent to 463-million kg-CO2. Projects for new energy introduction were studied, which included photovoltaic power generation for primary and junior high school buildings, the new city office, road signs along the municipal roads, and the harbor area; hybrid photovoltaic/wind power generation for a newly built fish market; solar heat utilization for heating frozen roads; wind power generation for the Wayama and Naranoki stock farms and for the harbor area; and a business for utilizing waste heat discharged by Kamaishi City's Eco-Town project. (NEDO)

  13. FY1998 research report on the basic research on geothermal district heating in Kamchatka, Russia; 1998 nendo Roshia Renpo Kamchatka shu ni okeru chinetsu riyo ni yoru chiiki danbo ni kansuru kiso chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-05-01

    Petropavlovsk-Kamchatky (P-K) city in Kamchatka, Russia is operating hot-water district heating using heavy oil boilers and waste hot water of thermal power plants as heat sources. Feasibility study was made on district heating using natural geothermal hot water and/or geothermal heat pump systems as heat sources of hot water supply for reduction of greenhouse effect gas emission. Among 3 areas including geothermal hot water, use of hot water in K area was impossible because of lower temperature and less spring water. Use of hot water in P and UP areas was impossible as primary hot water because of temperature drop to 64 degrees C during hot water supply toward P-K city. The building heating operation test was carried out using the geothermal heat pump system installed in a newly drilled heat exchange well of 100m deep. As a result, sufficient heat recovery was achieved for heating. If all of 49 boiler houses for heating are replaced with such geothermal heat pump systems, CO{sub 2} reduction was estimated to be 520,000t/y. (NEDO)

  14. New energy visions for the Town of Naie district. Human-friendly Town of Naie City by environment-friendly energies; Naiemachi chiiki shin energy vision. Kankyo ni yasashii energy ga tsukuru hito ni yasashii machi Naie

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The new energy visions are drawn for Town of Naie, Sorachi-gun in Hokkaido, where the industrial sector (manufacturing industries) consumes more than 60% of the total energy consumption. By energy type, oil and others account for 80%. When arranged in the order of expected recoverable quantity, the new energies for the town include utilization of solar energy, photovoltaic power generation, wind power generation, and utilization of wastes, biomass energy (agricultural products) and snow energy. The basic guidelines for introduction of new energies are for health and welfare, clearing and utilization of snow, environmental education and bringing up talented persons, supporting the related entrepreneurs, and integration of new energy with promotion of energy-saving movement. The priority projects the town plans to promote include utilization of biogas and temperature-differential energy for the town's water purification center; foundation of the Naie eco-school; and drawing conceptions for utilization of underground heat, utilization of low-temperature and snow/ice energy, co-energy around the spa of Naie, and mega-solar and eco-park at the Sorachi housing complex. (NEDO)

  15. Geothermal development promotion survey. No.B-4 'Kuenohira-yama area'; 1997 nendo chinetsu kaihatsu sokushin chosa data shori hokokusho. No.B-4 Kuenohira-yama chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-01-01

    The above-named survey had been scheduled to be conducted in fiscal years 1996-1998 for an approximately 10km{sup 2}-large area of Kokonoe-cho, Kusu-gun, Oita Prefecture. In fiscal 1996, surface surveys were conducted, such as geological and alteration zone surveys, gravity prospecting, and electromagnetic survey. In fiscal 1997, an exploration well (N9-KH-1: depth 2,222.0m) was drilled. Since the maximum temperature in the well was found to be so low as 78.5 degrees C, the survey was given up in the second year. The survey conducted at N9-KH-1 showed that deep-seated and shallow-seated ruptures developed there in the absence of aquicludes, and so it was inferred that it was a recharge area, as predicted by the temperature logging profile, with surface water flowing into the depth. The above showed that there was no deep-seated blind geothermal system which the survey was after. Since it was not likely that the shallow-seated geothermal system located in the southern part of the Mizuwake pass would be found expanding into the area of the current survey, it was decided that the survey not be carried forward to the following fiscal year. (NEDO)

  16. New energy vision of the Chuo town area. Creation of the town of good rapport and the environment; 2001 nendo Okayama ken Chuo cho chiiki shin energy vision. Fureai to junkan no machidukuri

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    For the purpose of promoting the introduction of new energy and enhancing the awareness of it in Chuo Town, Okayama Prefecture, an investigational study was conducted of the state of energy utilization of the town, project for new energy introduction, etc., and a new energy vision was worked out. The energy consumption amount in Chuo Town was estimated at 68,482 drums in terms of oil drum, and the amount of CO2 emitted from these was 13,287 t-CO2. To reduce this, the introduction of new energy and energy conservation were studied. As a result, the following were indicated as measures to be originally taken: introduction of solar light/solar heat use equipment to general households; installation of the regional cooperation power plant; each household's keeping of a household account book for the environment; introduction of energy-saving navigation device; activities for preservation of forest/terraced paddy field; ticket for exchange of forest volunteer work for products derived from the forest; campaign for composting of garbage using corrugated cardboard; recovery/recycle of waste cooking oil; full-of-flower gardening 500 movement; regionally consistent environmental education of parent/child participating type; introduction of micro hydraulic turbine; manufacture of products from thinnings by biomass lumber drying; expansion of special products of the area; method of the environmental management, etc. (NEDO)

  17. Fiscal 1999 geothermal development promotion survey report. Structural boring survey work and analysis (No. B-5 - Musadake region); 1999 nendo chinetsu kaihatsu sokushin chosa. Kozo shisui chosa koji oyobi kaiseki hokokusho - No. B-5 Musadake chiiki

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-12-01

    For geothermal development in the Musadake region, Shibetsu-gun, Hokkaido, structural boring was carried out and boreholes were inspected. In the structural boring survey, borehole N11-MD-3 was drilled as deep as 2,001.18 m, and borehole N11-MD-4 2001.44 m. Performed or tested for each of the two boreholes were temperature logging, electrical logging, temperature recovery, reinjection, core cutting examination, and so forth. The result of the temperature logging shows that temperature rise is slower at levels deeper than 1280 m in borehole N11-MD-3, with the maximum temperature marking 274 degrees C, and, in borehole N11-MD-4, the maximum temperature is 198 degrees C. No reinjection test is carried out because the ground pressure is too high to allow the replacement of the borehole content with fresh water. Analysis results lead to a conclusion that the hot fluid in this region originates in fossil seawater and in meteoric water, the latter arriving after a long-term permeation process, heated by thermal conduction to 270 degrees C or higher. It is inferred that the geothermal fluid flows in a fissure in the vicinity of the Musadake/Mt. Shitabanupuri fault and a fault orthogonally crossing the said fault for the formation of a high-temperature geothermal storage. (NEDO)

  18. Overall analysis report on the fiscal 1994 geothermal development promotion survey. No.C-2. Wasabizawa area (secondary); 1994 nendo chinetsu kaihatsu sokushin chosa sogo kaiseki hokokusho. No.C-2. Wasabizawa chiiki (dainiji)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-01-01

    The Wasabizawa area is located near the boundary between Yuzawa city south of Akita pref. and Ogachi-machi, Ogachi-gun, Akita pref, having a range of 3.0km northeast-southwest and 2.5km northwest-southeast of Wasabizawa almost as a center. The area for survey and its circumference are national forests, and the east and a part of the south of the area are designated as the Kurikoma quasi-national park third type special region. From the geochemical temperature distribution, it can be assumed that geothermal fluids of around 300{degree}C exist in the deep underground of the Kaminotai-Wasabizawa-Akinomiya area. From the distribution of density basements, the block structure of the basement can be estimated. Around the boundary of the ridge/sedimentation region of this density basement, fractures develop and the formation of reservoir structure is expected from the comparison with the result of the well survey. In the blowout test, obtained were 284.3{degree}C and 83.05 kgf/cm{sup 2} at a depth of 1,300m. In the geothermal system, it is assumed that reservoir structures of geothermal fluids develop in the deep underground of the central part of the area. The heat source to the geothermal system is supposed to be the magma reservoir remaining in Takamatsudake volcanic rocks. 110 refs., 177 figs., 105 tabs.

  19. Survey report on project for drawing district energy-saving visions for Town of Aizu Wakamatsu. Preliminary survey; 2000 nendo Aizu Wakamatsu shi chiiki sho energy vision sakutei nado jigyo (shoki dankai chosa) chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-07-01

    The feasibility study is conducted for introduction of the district energy-saving measures in City of Aizu Wakamatsu, for which the actual situations of energy consumption and promising energy-saving measures are surveyed. The city has a typical basinal weather, cold in winter and very hot and humid in summer, and tends to have a longer air-condition period in a year. It is considered that there are many wasteful energy consumption items to be studied for reduction. In the area of moving means, dependence on public transportation systems is considered to be relatively low, suggesting that there are much space of reducing transportation energy by the measures such as self-imposed control on use of private cars, promotion of utilizing public transportation systems and fuel-saving type driving throughout the city. The promising energy-saving measures studied to verify the effects include introduction of PVM analyzers for establishing better cost/effect relationship between power consumed by air-conditioners and pleasantness; introduction of garbage power generation, co-generation and high-efficiency illuminators; improvement of power-factor; and demand control for air conditioning, rationalization of power-receiving facilities, introduction of systems for saving flushing water and improvement of air conditioners' outdoor facilities. (NEDO)

  20. Fiscal 2001 survey report on regional new energy vision formulation at Kyonan Town; 2001 nendo Kyonan machi shin energy vision sakutei chosa hokokusho. Chiiki shin energy vision sakutei tou jigyo (Watashitachi ga tsukuru machi, subete ga koen no machi)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-03-01

    A regional new energy vision was formulated at Kyonan Town, Chiba Prefecture, which includes a new energy introduction plan, an action program for the plan, and the like, intended to promote enlightenment/popularization relative to new energy and environmental matters. Kyonan Town consumes 18.69 times 10{sup 10} kcal of energy, with oil accounting for 65.4%, LPG for 6.0%, and electricity 28.6%, the statistics disclosing that the town has an oil-dependent energy consumption structure. The new energy likely to be introduced will comprise 13,574 times 10{sup 10} kcal/year in solar energy, 336,187 MWh/year in wind power, 17,170 kWh/year in small-scale hydroelectric power, 0.57 times 10{sup 10} kcal/year in refuse fueled power, and 0.60 times 10{sup 10} kcal/year in biomass fueled power. The town's new energy introduction project centers on photovoltaics, passive solar heat utilization, wind power, small-scale hydroelectric power, and aims to introduce the new energies into public facilities such as the Eco Garden and Katsuyama Primary School, and into kindergartens, nursery schools, shopping malls, and so forth. A plan was also worked out for adopting clean-energy vehicles for official use. (NEDO)

  1. New energy vision for Nomura town. Fiscal 2000 survey report on regional new energy vision formulation project for Nomura town, Ehime Prefecture; Nomuracho shin energy vision. 2000 nendo Nomuracho chiiki shin energy vision sakutei nado jigyo chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    An effort is being exerted to build a town which will generate but a little environmental impact, with symbiosis with nature established as the highest priority target of the town administrative activities. Under the Nomura town new energy vision, a basic policy and measures are established toward the promotion of new energy introduction, with local features, existence of new energy resources, and the feasibility of new energy adoption taken into consideration. Surveys were made and the results are compiled into seven chapters, which involve (1) the trend of new energy, (2) local features, (3) existing amount and available amount of new energy, (4) basic policy toward the introduction of new energy, (5) main projects for the introduction of new energy, (6) details of the basic policy toward the introduction of new energy, and (7) the Nomura Town Deliberation Committee on New Energy Vision Formulation. Chapter (6) covers the assessment of new energy introduction feasibility, promotion of concretized introduction projects, public relations/enlightening/popularizing activities, and assistance for new energy introduction. Studied for introduction are photovoltaic power generation, solar heat utilization, wind power generation, biomass power generation, and the like. (NEDO)

  2. Survey for working out a new energy vision of the Tateshina Town area; 2001 nendo Tateshina machi shin energy vision sakutei chosa hokokusho. Chiiki shin energy vision sakutei tou jigyo

    Energy Technology Data Exchange (ETDEWEB)

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    2002-02-01

    For the purpose of promoting the introduction of new energy and heightening the awareness of new energy in Tateshina Town, Kita-saku County, Nagano Prefecture, study was made of the existence amount of new energy, energy consumption amount in the town, project for the concrete introduction, etc., and a 'new energy vision of the area' was worked out. According to the sample survey, in the energy supply/demand structure of Tateshina Town, electricity was 32.2%, kerosene 23.9%, gasoline 17.1%, light oil 11.2%, heavy oil 10.3%, etc. By sector, the commercial/residential sector was 57.1%, transportation sector 25.9% and industrial sector 17.0%. The demand in FY 2010 is expected to increase by 8.9%-22.0% in FY 2010. For the project on new energy introduction, the following were studied: supply of new energy information, support of the introduction to town people, energy utilization of forest resource, preparation of an eco-museum in the Shin-jin'nai forest park, promotion of the tourism using new energy, study of energy utilization of stockbreeding waste, introduction to administrative facilities, etc. (NEDO)

  3. Report on regional new energy vision for Kawara town. Toward the creation of eco-symbiotic community (simplified version); Kawaracho chiiki shin energy vision gaiyoban. Kankyo kyoseigata shakai no sozo ni mukete

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    A regional new energy vision has been formulated for Kawara town, Fukuoka Prefecture, in the hope that the industry, administration, and citizenry will be concerned with each other for the realization of an eco-symbiotic community. The fruits of endeavors exerted for this purpose are described in seven chapters, which are (1) the outline of surveys, (2) local characteristics of Kawara town, (3) policy toward introducing new energy, (4) measures for introducing new energy, (5) study of model projects, (6) introduction of new energy into Kawara town, and (7) efforts to realize the introduction. Described in chapter (5) are the introduction of photovoltaic power generation into primary and junior high schools, heat supply business utilizing waste heat from a plant, energy conservation at administration buildings, development of an activities program for people to learn energy related matters, and the results of studies conducted on the respective model projects. Concerning the activities program, several applications are considered, dependent upon who are to implement the program: volunteer students, local community leaders, experts, or administration officials. For the initial stage of learning under the program, a system is built under which experts, local community leaders, primary and junior highschool children, and their teachers cooperate with each other under the leadership of a project coordinator. (NEDO)

  4. Survey report. New energy vision formulation project for Osa Town, Okayama Prefecture; 2001 nendo Osa cho shin energy vision sakutei tou jigyo chosa hokokusho. Chiiki shin energy vision sakutei tou jigyo

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    A New Energy Vision was formulated for Osa Town after investigations about energy demand, new energy reserves, and their available amounts. The town consumes approximately 50.6-billion kcal in total, with automobiles consuming a half thereof as fuel. Studies were made about new energy reserves and recoverable amounts in the town, and then an estimation was made that 16.2 times 10{sup 5} kWh/year would be recoverable from photovoltaic energy, 1.58 times 10{sup 5} kWh from small-scale hydropower, 8.79 times 10{sup 6} kWh from ligneous biomass, and 1.96 times 10{sup 5} kWh from livestock biomass, and that 40 MW, for example, would come from wind power, subject to change depending on the wind power generator system to be built. Projects for new energy introduction were discussed, which involved the use of biodiesel fuel for official vehicles, installation of solar hot water showers in primary and junior high schools, application of photovoltaic/wind power to crime prevention lights and street lamps, snow melting systems for roads utilizing geothermal energy, use of heat from thinnings and ligneous waste, wind power facilities, small-scale hydropower facilities, introduction of new energy appliances into sightseeing facilities, and the introduction of photovoltaic power or the like into public facilities. (NEDO)

  5. FY 1998 annual report on the survey report on possibility of international joint researches for Asian countries. 5; 1998 nendo Asia chiiki kokusai kyodo kenkyu kanosei chosa hokokusho. 5

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    Described herein are results of surveys on dissemination policies for, and development trends in, energy saving and new energy areas in 6 Asian countries, Thailand, Indonesia, Malaysia, the Philippines, India and China. Possible joint research themes for Indonesia include effective utilization of palm oil residue, modernization of distribution systems, high-efficiency coal-fired thermal power generation techniques, and promotion of dissemination of solar cells. For Malaysia, renewable energies, e.g., photovoltaic power, will be developed to promote electrification for those areas not well electrified. The Philippines is positively receiving technical and financial aids for new energy and energy-saving areas from the advanced countries. India is considering to introduce a number of projects for, e.g., technological optimization of power supply area, decentralized power generation, oil exploitation and extraction techniques, and photovoltaic power generation. China is still in the preliminary stage in its energy research and development, and will need further joint researches in the energy sector. (NEDO)

  6. Report on achievements in fiscal 1974 in Sunshine Project. Study on hot water systems in geothermal areas; 1974 nendo chinetsu chiiki no netsusuikei ni kansuru kenkyu seika chukan hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1977-03-01

    This study has begun in fiscal 1973 under a five year plan with an objective to elucidate origin and maintenance of reservoirs of geothermal fluids. To achieve the objective, estimation was made on the systems of infiltration, storage and gushing of the fluids, particularly on infiltration areas. In the hydraulic flow rate study, observation was carried out in the Onikubi area on amount of rainfall, air pressures, temperatures, electric conductivity, and pH, and in ten fluid sources on flow rates, temperatures and pH. Flow rate observation was started at three rivers. In the Kuju area, flow rate observation was started on four fluid sources. Observations were started on temperatures, electric conductivity, flow rates, amount of rainfall by using the Takenoyu geothermal steal wells, and on amount of rainfall in the Teraono and Hacchobara areas. In the study of isotopic geology, site analyses and water collection were carried out in the Kuju area for underground water in six locations, hot spring water in seven locations, and 17 test samples from two geothermal wells. As a study on reservoirs, observation was started in the southern Hachimantai area on measurement of ground fluctuation in association with steam collection. In parallel, fracture survey and gravity measurement were carried out. In order to investigate transformed geology, analytic samples were collected from 12 survey wells in the Onikubi area. A spinner flow mater was tested in that area. (NEDO)

  7. Investigations in fiscal 1986 on promotion of geothermal development. Part 2. Report on precision structural test well drilling and investigation works in Otaki area; 1986 nendo chinetsu kaihatsu sokushin chosa hokokusho. 2. Otaki chiiki seimitsu kozo shisui oyobi chosa koji

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1987-09-01

    With an objective of promotion of geothermal development in the Otaki area in Nagano Prefecture, precision structural test well drilling and investigation works have been carried out, and the result thereof reported. The investigated area is an inclined land with an area of 70 km{sup 2} at altitudes of 1,000 to 2,000 m in the southern foot of the Mitake volcano having Otaki River as the southern border. The investigation has performed during the period from March 2 to September 30, 1987 the drilling of the N61-OT-5 test well hole (depth of 502.0 m), temperature logging, temperature restoration test, water injection test, and core test. The result of the investigation may be summarized as follows: existence of the stratigraphic succession as predicted by ground surface investigation, and existence of the Mesozoic and Palaeozoic strata of the Mino belt were verified; the Mesozoic and Palaeozoic strata of the Mino belt being the base bed have only small cracks, and very little alteration can be identified; X-ray diffraction of powder from test drilled cores recognized very little minerals altered by hot water; and the temperature logging and temperature restoration test identified no temperature indication that clearly indicates existence of heat sources. As a conclusion, there is no alteration due to hot water down to 500 m below the surface in the vicinity of the investigation well, and the possibility of a geothermal reservoir to exist is extremely low. (NEDO)

  8. FY 2000 report on the investigation of environmental effects in the geothermal development promotion survey. Tertiary. No.B-7 Kuwanosawa area; 2000 nendo chinetsu kaihatsu sokushin chosa. Kankyo eikyo chosa hokokusho - No. B-7 Kuwanosawa chiiki (Dai 3 ji)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-01-01

    In the Kuwanosawa area, Yuzawa city, Akita prefecture, survey was conducted to grasp effects of drilling for structural boring in the geothermal survey on the peripheral environment. In the survey of animals, there were 5 orders/10 families/19 species of mammals and 10 orders/25 families/73 species of birds. Three kinds of precious animals were confirmed. There were 132 families/6,670 species in the flora. Three kinds of precious plants and three kinds of precious floras were confirmed. During the survey, drilling for structural boring was conducted. At three spas for survey, there were recognized some survey items of which there were great fluctuations. However, those are seasonal valuations or valuations related to the situation of the inside of hot springs. It is not recognized that the fluctuations were caused by the drilling work and pumping-up of geothermal water. The noise made during the well drilling was reduced around the well as theoretical values indicated. At a spot 200m away from the well, the noise was reduced from the environmental standard, and accordingly, it seems that there are few effects on the peripheral environment. The vibration was reduced around the well more than theoretical values indicated. That was below the control level at a spot 25m away from the well, and it seems that there are few effects on the peripheral environment. (NEDO)

  9. Report on FY 2000 project for drawing district energy-saving visions for Village of Satomi; 2000 nendo Satomi-mura chiiki sho energy vision sakutei nado jigy hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-03-01

    The district energy-saving visions are drawn for Village of Satomi, aimed at construction of an energy-saving, resources-recycling type farming village in mountains, friendly to the environments, and the implementation organization is established. The activities include (1) questionnaire survey for energy saving, with the questionnaires sent to the citizens and enterprises, (2) actual situations of energy consumption, (3) setting the hardware and software targets achievable in 5 years, (4) setting the projects for introducing energy-saving facilities, and (5) establishment of the energy-saving spreading committee, and drawing the action programs. Insufficient practices are pointed out, and necessity for the spread/enlightenment activities are clarified. At the same time, it is estimated that the energy consumption can be potentially reduced by 8% in 5 years. It is decided to introduce a co-generation system in the biomass recycling center, systems for photovoltaic power generation and production of hot water by solar heat in the integrated day-care center, and wind power system in Plateau Satomi. (NEDO)

  10. Building a network of flowers, green, and ocean. Investigation report on establishment of new energy vision in Wada Town area (Chiba Prefecture); Hana midori umi no network zukuri. Wadacho chiiki shin energy vision sakutei chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    With the idea of living together with well-blessed nature placed as the base of building the town, new energy utilization promoting measures were established spanning over ten years starting fiscal 2000. Specifically, the vision was established with the following points as the substance: introduction of new energy technologies into the agricultural and fishery industries as the basic district industry, initiative introduction into public facilities in consideration of repercussion effects brought about by the introduction, provision of energy related information by improving publicity magazines and information technologies, and expanded provision of environmental information collaborated with schools. Specific measures discussed include photovoltaic power generation, utilization of bio-mass energies made available from livestock excreta, and algae, utilization of hydro-energy through the improvement project of the Komukai dam, utilization of wind power energy and ocean wave power energy, and utilization of rape-seed oil as automotive fuel. Such educational activities will also be promoted actively as the setting of economy indexes, assistance to autonomous activities, and holding training meetings, seminars, and experience education class rooms. (NEDO)

  11. FY 2000 Project report on survey for drawing district energy-saving visions for City of Oguchi; 2000 nendo Oguchi-shi chiiki sho energy vision sakutei nado jigyo chosa hokokusho

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2001-02-01

    The energy-saving visions are drawn for City of Oguchi to help spread the energy-saving practices through the citizens, based on the studies on more efficient and realizable energy-saving measures, including effective utilization of district energy. The energy-saving activities cover the following 6 areas: (1) energy demand trends and current status of energy-saving measures, (2) basic environmental conditions related to energy saving in the district, (3) prediction of energy demands, (4) basic conception of the district energy saving, and introduction methodology, (5) energy-saving implementation plans, and (6) promotion of and problems involved in introduction of the energy-saving measures. The energy-saving implementation plans cover the public facilities, household, schools and private enterprises, and the energy-saving measures studied for the public facilities include introduction of microhydroelectric power generation systems for service water and sewer systems, revolving doors for libraries, high-efficiency illumination systems, and various types of solar systems. Those for household include economic utilization of home electric appliances and automobiles, and those for cooperation by the administration and citizens include thorough separation of garbage. (NEDO)

  12. FY 1998 geothermal development promotion survey. Report on the environmental effect survey (No. A-4 Kunbetsu-dake area); 1998 nendo chinetsu kaihatsu sokushin chosa. Kankyo eikyo chosa hokokusho (No.A-4 Kunbetsudake chiiki)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-09-30

    Of the FY 1998 geothermal development promotion survey, the primary environmental effect survey in the Kunbetsu-dake area was conducted, and the results were arranged. In the well drilling survey, etc., the survey was made aiming at extracting areas to be preserved from an environmental aspect. The results of the survey were summarized as follows: As to the mammalia, 12 families 46 species were confirmed in the area surveyed and the periphery by literature survey. Out of them, the noticeable species which are considered important from an academic viewpoint are 10 families 19 species. As to the aves, 55 families 340 species were confirmed, of which 29 families 79 species are noticeable species. About the amphibia/reptilia, 3 families 3 species in the amphibia and 4 families 6 species in the reptilia were confirmed by literature and hearing survey. The noticeable species is 1 family 1 species in the amphilia. Concerning the terrestrial insecta, 135 families 873 species were confirmed, and the noticeable species is 11 families 20 species. As to the flora, 115 families 1055 species were confirmed, and the noticeable species is 46 families 126 species. Relating to the living vegetation, the one higher than 9 in nature reserves shows a substantial rate, and the periphery of Kaibetsu-dake is designated as the specified flora colony. (NEDO)

  13. FY 1992 report on the survey of geothermal development promotion. Electromagnetic exploration (TDEM method) (No. A-1 - Haneyama area); 1992 nendo chinetsu kaihatsu sokushin chosa chijo chosa hokokusho futai shiryo. Denji tansa (TDEM ho) hokokusho (No. A-1 Haneyama chiiki)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1994-03-01

    For the purpose of elucidating a possibility of existence of geothermal reservoirs in the Haneyama area, Oita Prefecture, Electromagnetic survey by the TDEM method was conducted. The survey was made for the area of 80km{sup 2} at 105 measuring points in the south of the Haneyama area, and the resistivity structure reaching the depth of 4,000m from the earth surface was made clear. The resistivity structure in this area is composed of 4 layers: upper medium resistivity layer, central medium resistivity layer, central low resistivity layer and lower high resistivity layer. The resistivity structure discontinuous line ranges between the uplift zone of basement and the sedimentation zone, and the greater part of the alteration zone is distributed and arranged concomitant with the position and direction of the line. As the region with high potential of geothermal existence, cited were the low resistivity zone distributed in the west of the resistivity structure discontinuous line that continues from Mt. Fukuman to the south, the low resistivity zone distributed in the north of the resistivity structure discontinuous line from Noya to the west, and the low resistivity zone distributed in the north of Hosenji and lying between resistivity structure discontinuous lines. (NEDO)

  14. Achievement report for fiscal 1998 on area consortium research and development business, 1st year. Area consortium energy research and development (ultrahigh density information storage components); 1998 nendo chiiki consortium kenkyu kaihatsu. Chokomitsudo joho storage component (dai 1 nendo)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-03-01

    The effort aims to prepare the base of a next-generation ultrahigh density HDD (hard disk drive) industry in the Tohoku district. The contact head was modeled as a cantilever beam and a head disk linkage impact model for analysis was built for the elucidation of the effects of various factors. A microactuator using a PZT (piezoelectric transducer) thin film was evaluated concerning the constitution of its elements, and durability. Materials design guidelines were indicated concerning the moderation and suppression of thermomagnetism in the ultrahigh density magnetic recording medium. A grain diameter of less than 10nm was found necessary for a decrease in the interaction between magnetic crystal grains for the achievement of a reduction in noise, and a material was produced that met the condition. An appropriate abrasive was chosen for superfine surface processing. Attention was paid to the chemical loss of lubricating oil to cause lethal destruction of HDI (hard disk interface) and , after evaluating hard film and frictional conditions, it was found that a hard layer and soft layer were required to complete an effective lubricant film. A ferroelectric body and a shape memory alloy were combined into a composite lamination and crystal orientation therein was controlled, and the lamination showed its feasibility as a thin film actuator. (NEDO)

  15. Survey on acceleration of geothermal development in fiscal 1998. Report on survey for influence on environment (No. B-7, Kuwanosawa district); 1998 nendo chinetsu kaihatsu sokushin chosa.Kankyo eikyo ch