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Sample records for chicory cichorium intybus

  1. Seasonal variation in sesquiterpene lactone concentration and composition of forage chicory (Cichorium intybus L.) cultivars

    Science.gov (United States)

    Chicory (Cichorium intybus L.) herbage contains sesquiterpene lactones that are believed to help control gastrointestinal nematodes in small ruminants. Because the anthelmintic effect could be related to both total sesquiterpene lactone concentration and the proportions of the principal constituent...

  2. Spontaneous gene flow and population structure in wild and cultivated chicory, Cichorium intybus L.

    OpenAIRE

    Kiaer, L.P.; Felber, F.; Flavell, A.; Guadagnuola, R.; Guiatti, D.; Hauser, T.P.; Olivieri, A. M.; Scotti, I.; Syed, N; Vischi, M.; Wiel, van de, L.; Jorgensen, R.B.

    2009-01-01

    Spontaneous gene flow between wild and cultivated chicory, Cichorium intybus L. , may have implications for the genetic structure and evolution of populations and varieties. One aspect of this crop-wild gene flow is the dispersal of transgenes from genetically modified varieties, e.g. gene flow from GM chicory to natural chicory could have unwanted consequences. With the purpose to identify and quantify crop-wild gene flow in chicory, we analysed introgression in 19 wild chicory populations a...

  3. The biosynthesis of sesquiterpene lactones in chicory (Cichorium intybus L.) roots

    NARCIS (Netherlands)

    Kraker, de J.W.

    2002-01-01

    Wild chicory(Cichorium intybusL.) is a blue-flowered composite plant that has spread all over the world from the Mediterranean. Sprouts of chicory var.Spontaneous gene flow and population structure in wild and cultivated chicory, Cichorium intybus L

    DEFF Research Database (Denmark)

    Kiær, Lars Pødenphant; Felber, F.; Flavell, A.;

    2009-01-01

    Spontaneous gene flow between wild and cultivated chicory, Cichorium intybus L., may have implications for the genetic structure and evolution of populations and varieties. One aspect of this crop-wild gene flow is the dispersal of transgenes from genetically modified varieties, e.g. gene flow from...... GM chicory to natural chicory could have unwanted consequences. With the purpose to identify and quantify crop-wild gene flow in chicory, we analysed introgression in 19 wild chicory populations and 16 accessions of chicory varieties and landraces distributed across Northern, Central and...

  4. Red Chicory (Cichorium intybus L. cultivar) as a Potential Source of Antioxidant Anthocyanins for Intestinal Health

    OpenAIRE

    Laura D'Evoli; Fabiana Morroni; Ginevra Lombardi-Boccia; Massimo Lucarini; Patrizia Hrelia; Giorgio Cantelli-Forti; Andrea Tarozzi

    2013-01-01

    Fruit- and vegetable-derived foods have become a very significant source of nutraceutical phytochemicals. Among vegetables, red chicory (Cichorium Intybus L. cultivar) has gained attention for its content of phenolic compounds, such as the anthocyanins. In this study, we evaluated the nutraceutical effects, in terms of antioxidant, cytoprotective, and antiproliferative activities, of extracts of the whole leaf or only the red part of the leaf of Treviso red chicory (a typical Italian red leaf...

  5. Identification of novel genes potentially involved in somatic embryogenesis in chicory (Cichorium intybus L.)

    OpenAIRE

    Lucau-Danila, Anca; Laborde, Laurent; Legrand, Sylvain; Huot, Ludovic; Hot, David; Lemoine, Yves; Hilbert, Jean-Louis; Hawkins, Simon; Quillet, Marie-Christine; Hendriks, Theo; Blervacq, Anne-Sophie

    2010-01-01

    Background In our laboratory we use cultured chicory (Cichorium intybus) explants as a model to investigate cell reactivation and somatic embryogenesis and have produced 2 chicory genotypes (K59, C15) sharing a similar genetic background. K59 is a responsive genotype (embryogenic) capable of undergoing complete cell reactivation i.e. cell de- and re-differentiation leading to somatic embryogenesis (SE), whereas C15 is a non-responsive genotype (non-embryogenic) and is unable to undergo SE. Pr...

  6. Comparison of the Phenolics Profiles of Forced and Unforced Chicory (Cichorium intybus L.) Cultivars

    OpenAIRE

    Lovro Sinkovič; Janez Hribar; Dragan Znidarčič; Dieter Treutter; Rajko Vidrih

    2014-01-01

    Modern and sustainable production of safe and healthy high quality vegetables for human consumption is nowadays the goal of many producers. Chicory (Cichorium intybus L.) has become an important crop used as food in many countries over the past decade. In this work field and glasshouse trials were conducted to compare phenolic profiles of hydroponically forced leaves and leaves produced as common agronomic practice of five chicory cultivars. Total phenolic content of hydroponically for...

  7. The biosynthesis of sesquiterpene lactones in chicory (Cichorium intybus L.) roots

    OpenAIRE

    Kraker, de, J.

    2002-01-01

    Wild chicory(Cichorium intybusL.) is a blue-flowered composite plant that has spread all over the world from the Mediterranean. Sprouts of chicory var.foliosumHegi that are grown in the dark became popular as a vegetable (Belgian endive) halfway through the nineteenth century. Nowadays it is a common crop in Belgium, northern France, and the Netherlands. The well-known bitter taste of chicory is associated with the presence of sesquiterpene lactones of which the three major ones are the guaia...

  8. Influence of chicory roots (Cichorium intybus L) on boar taint in entire male and female pigs

    OpenAIRE

    Hansen, L L; Mejer, H.; Thamsborg, S.M.; Burne, D.V.; Roepstorff, A.; Karlsson, A H; Hansen-Møller, J.; Jensen, M.T.; Tuomola, M.

    2005-01-01

    Boar taint is an off-flavour of pork caused primarily by a microbial breakdown product, skatole and a testicular steroid, androstenone. As skatole is produced in the large intestine from tryptophan, it is possible that some "bioactive" ingredients could modify protein fermentation and, in the process, diminish boar taint. The aim of this study was to examine the effect of inulin-rich chicory roots (Cichorium intybus L.) on boar taint. In the first of three trials individually penned, entire m...

  9. Chicory (Cichorium intybus L.) and cereals differently affect gut development in broiler chickens and young pigs

    OpenAIRE

    Liu, Haoyu; Ivarsson, Emma; Lundh, Torbjörn; Lindberg, Jan Erik

    2013-01-01

    Dietary fiber, resistant to host-mediated digestion in the small intestine due to lack of endogenous enzymes, impacts many facets of animal health and is associated with gut development especially in young monogastrics. Furthermore, it can be used as in-feed antibiotic alternative. Chicory (Cichorium intybus L.) forage with high content of pectin (uronic acids as building blocks) is a novel class of dietary fiber that is chemically different from cereal grains (with high content of arabinoxyl...

  10. Genetic determinism of inulin metabolism in industrial chicory (Cichorium intybus L.) : an association mapping approach

    OpenAIRE

    Raulier, Pierre

    2015-01-01

    Inulin is a fructose polymer extracted from the root of industrial chicory (Cichorium intybus L.). The quality of the root extract is determined by the polymerization degree of inulin molecules and the free fructose concentration. Low autumnal temperatures trigger an inulin degradation phase which decreases the quality of the root extract. Therefore, the objective of this thesis was to understand the genetic determinism of the synthesis and the cold induced degradation of inulin. To do so, we...

  11. Phytochemical, antioxidant and mineral composition of hydroalcoholic extract of chicory (Cichorium intybus L.) leaves

    OpenAIRE

    Abbas, Zahid Khorshid; Saggu, Shalini; Sakeran, Mohamed I.; Zidan, Nahla; Rehman, Hasibur; Abid A. Ansari

    2014-01-01

    The phytochemical, antioxidant and mineral composition of hydroalcoholic extract of leaves of Cichorium intybus L., was determined. The leaves were found to possess comparatively higher values of total flavonoids, total phenolic acids. The phytochemical screening confirmed the presence of tannins, saponins, flavonoids, in the leaves of the plant. The leaf extract was found to show comparatively low value of IC50 for 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition. The IC50 value of chicory le...

  12. Fatty acid composition of leaves of forced chicory (Cichorium intybus L.)

    OpenAIRE

    Sinkovič Lovro; Hribar Janez; Vidrih Rajko; Ilin Žarko M.; Žnidarčič Dragan

    2015-01-01

    The objective of the present study was to determine the composition of fatty acids in leaves of nine chicory cultivars (Cichorium intybus L.). The growing practice followed the traditional forcing method of developed roots in a peat to obtain new etiolated vegetative apical buds, known as chicons. The fatty acid content was determined by the extraction of fatty acid methyl esters and analysis by means of gas chromatography. The analysis revealed the following ...

  13. In vitro analysis of the anthelmintic activity of forage chicory (Cichorium intybus L.) sesquiterpene lactones against a predominatly Haemonchus contortus egg population

    Science.gov (United States)

    The anthelmintic activity of chicory (Cichorium intybus L.) herbage has been attributed to sesquiterpene lactones. Chicory leaves contain significant amounts of lactucin (LAC), 8-deoxylactucin (DOL), and lactucopicrin (LPIC), but the proportions of these three sesquiterpene lactones vary among fora...

  14. Phytochemical, antioxidant and mineral composition of hydroalcoholic extract of chicory (Cichorium intybus L.) leaves.

    Science.gov (United States)

    Abbas, Zahid Khorshid; Saggu, Shalini; Sakeran, Mohamed I; Zidan, Nahla; Rehman, Hasibur; Ansari, Abid A

    2015-05-01

    The phytochemical, antioxidant and mineral composition of hydroalcoholic extract of leaves of Cichorium intybus L., was determined. The leaves were found to possess comparatively higher values of total flavonoids, total phenolic acids. The phytochemical screening confirmed the presence of tannins, saponins, flavonoids, in the leaves of the plant. The leaf extract was found to show comparatively low value of IC50 for 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition. The IC50 value of chicory leaves extract was found to be 67.2 ± 2.6 μg/ml. The extracts were found to contain high amount of mineral elements especially Mg and Zn. Due to good phytochemical and antioxidant composition, C. intybus L., leaves would be an important candidate in pharmaceutical formulations and play an important role in improving the human health by participating in the antioxidant defense system against free radical generation. PMID:25972754

  15. Identification of novel genes potentially involved in somatic embryogenesis in chicory (Cichorium intybus L.)

    OpenAIRE

    Quillet Marie-Christine; Hawkins Simon; Hilbert Jean-Louis; Lemoine Yves; Hot David; Huot Ludovic; Legrand Sylvain; Laborde Laurent; Lucau-Danila Anca; Hendriks Theo; Blervacq Anne-Sophie

    2010-01-01

    Abstract Background In our laboratory we use cultured chicory (Cichorium intybus) explants as a model to investigate cell reactivation and somatic embryogenesis and have produced 2 chicory genotypes (K59, C15) sharing a similar genetic background. K59 is a responsive genotype (embryogenic) capable of undergoing complete cell reactivation i.e. cell de- and re-differentiation leading to somatic embryogenesis (SE), whereas C15 is a non-responsive genotype (non-embryogenic) and is unable to under...

  16. Antioxidants in Varieties of Chicory (Cichorium intybus L.) and Wild Poppy (Papaver rhoeas L.) of Southern Italy

    OpenAIRE

    Anna Montefusco; Giuliana Semitaio; Pier Paolo Marrese; Andrea Iurlaro; Monica De Caroli; Gabriella Piro; Giuseppe Dalessandro; Marcello Salvatore Lenucci

    2015-01-01

    We report the hydrophilic and lipophilic antioxidant activities, as well as the total phenol, flavonoid, tocochromanol (tocopherol and tocotrienol), and carotenoid contents in the edible portion of wild and cultivated varieties of chicory (Cichorium intybus L.) and in the basal rosette leaves of the wild species of poppy (Papaver rhoeas L.), known by natives as “paparina,” collected in the countryside of Salento (South Apulia, Italy). We analyzed (1) two cultivars of chicory, the “Catalogna” ...

  17. Comparison of the Phenolics Profiles of Forced and Unforced Chicory (Cichorium intybus L. Cultivars

    Directory of Open Access Journals (Sweden)

    Lovro Sinkovič

    2014-09-01

    Full Text Available Modern and sustainable production of safe and healthy high quality vegetables for human consumption is nowadays the goal of many producers. Chicory (Cichorium intybus L. has become an important crop used as food in many countries over the past decade. In this work field and glasshouse trials were conducted to compare phenolic profiles of hydroponically forced leaves and leaves produced as common agronomic practice of five chicory cultivars. Total phenolic content of hydroponically forced samples ranged from 60 to 140 mg/100 g fresh weight, and those of unforced grown leaves varied between 117 and 386 mg/100 g fresh weight. Red cultivar ‘Treviso’ shows the highest phenolic content for both, unforced and forced leaves extracts. In the unforced leaves a total of 33 and in those of forced a total of 44 compounds (peaks were used for the discrimination study.

  18. Red Chicory (Cichorium intybus L. cultivar as a Potential Source of Antioxidant Anthocyanins for Intestinal Health

    Directory of Open Access Journals (Sweden)

    Laura D'evoli

    2013-01-01

    Full Text Available Fruit- and vegetable-derived foods have become a very significant source of nutraceutical phytochemicals. Among vegetables, red chicory (Cichorium Intybus L. cultivar has gained attention for its content of phenolic compounds, such as the anthocyanins. In this study, we evaluated the nutraceutical effects, in terms of antioxidant, cytoprotective, and antiproliferative activities, of extracts of the whole leaf or only the red part of the leaf of Treviso red chicory (a typical Italian red leafy plant in various intestinal models, such as Caco-2 cells, differentiated in normal intestinal epithelia and undifferentiated Caco-2 cells. The results show that the whole leaf of red chicory can represent a good source of phytochemicals in terms of total phenolics and anthocyanins as well as the ability of these phytochemicals to exert antioxidant and cytoprotective effects in differentiated Caco-2 cells and antiproliferative effects in undifferentiated Caco-2 cells. Interestingly, compared to red chicory whole leaf extracts, the red part of leaf extracts had a significantly higher content of both total phenolics and anthocyanins. The same extracts effectively corresponded to an increase of antioxidant, cytoprotective, and antiproliferative activities. Taken together, these findings suggest that the red part of the leaf of Treviso red chicory with a high content of antioxidant anthocyanins could be interesting for development of new food supplements to improve intestinal health.

  19. Red chicory (Cichorium intybus L. cultivar) as a potential source of antioxidant anthocyanins for intestinal health.

    Science.gov (United States)

    D'evoli, Laura; Morroni, Fabiana; Lombardi-Boccia, Ginevra; Lucarini, Massimo; Hrelia, Patrizia; Cantelli-Forti, Giorgio; Tarozzi, Andrea

    2013-01-01

    Fruit- and vegetable-derived foods have become a very significant source of nutraceutical phytochemicals. Among vegetables, red chicory (Cichorium Intybus L. cultivar) has gained attention for its content of phenolic compounds, such as the anthocyanins. In this study, we evaluated the nutraceutical effects, in terms of antioxidant, cytoprotective, and antiproliferative activities, of extracts of the whole leaf or only the red part of the leaf of Treviso red chicory (a typical Italian red leafy plant) in various intestinal models, such as Caco-2 cells, differentiated in normal intestinal epithelia and undifferentiated Caco-2 cells. The results show that the whole leaf of red chicory can represent a good source of phytochemicals in terms of total phenolics and anthocyanins as well as the ability of these phytochemicals to exert antioxidant and cytoprotective effects in differentiated Caco-2 cells and antiproliferative effects in undifferentiated Caco-2 cells. Interestingly, compared to red chicory whole leaf extracts, the red part of leaf extracts had a significantly higher content of both total phenolics and anthocyanins. The same extracts effectively corresponded to an increase of antioxidant, cytoprotective, and antiproliferative activities. Taken together, these findings suggest that the red part of the leaf of Treviso red chicory with a high content of antioxidant anthocyanins could be interesting for development of new food supplements to improve intestinal health. PMID:24069504

  1. Chicory (Cichorium intybus L) as fibre source in pig diets

    OpenAIRE

    Ivarsson, Emma

    2012-01-01

    The aim of this thesis was to increase our understanding about chicory (forage and root) as fibre source for pigs, by studying the effects of diets with inclusion of chicory on digestibility, digestion site, performance, gut microbiota and environment. In total 62 pigs were used, including newly weaned and growing intact pigs and growing post valve t-caecum (PVTC)-cannulated pigs. The weaned pigs were fed cereal-based diets with inclusion of 40, 80 and 160 g/kg of either chicory or ribwor...

  2. Genetic diversity and relationship of chicory (Cichorium intybus L.) using sequence-related amplified polymorphism markers.

    Science.gov (United States)

    Liang, X Y; Zhang, X Q; Bai, S Q; Huang, L K; Luo, X M; Ji, Y; Jiang, L F

    2014-01-01

    Chicory is a crop with economically important roles and is cultivated worldwide. The genetic diversity and relationship of 80 accessions of chicories and endives were evaluated by sequence-related amplified polymorphism (SRAP) markers to provide a theoretical basis for future breeding programs in China. The polymorphic rate was 96.83%, and the average polymorphic information content was 0.323, suggesting the rich genetic diversity of chicory. The genetic diversity degree of chicory was higher (GS = 0.677) than that of endive (GS = 0.701). The accessions with the highest genetic diversity (effective number of alleles, NE = 1.609; Nei's genetic diversity, H = 0.372; Shannon information index, I = 0.556) were from Italy. The richest genetic diversity was revealed in a chicory line (NE = 1.478, H = 0.289, I = 0.443) among the 3 types (line, wild, and cultivar). The chicory genetic structure of 8 geographical groups showed that the genetic differentiation coefficient (GST) was 14.20% and the number of immigrants per generation (Nm) was 3.020. A GST of 6.80% and an Nm of 6.853 were obtained from different types. This observation suggests that these chicory lines, especially those from the Mediterranean region, have potential for providing rich genetic resources for further breeding programs, that the chicory genetic structure among different countries obviously differs with a certain amount of gene flow, and that SRAP markers could be applied to analyze genetic relationships and classifications of Cichorium intybus and C. endivia. PMID:25299087

  3. Effect of Chemical and Organic Fertilisation on Common Chicory (Cichorium intybus L. in the First Vegetation Year

    Directory of Open Access Journals (Sweden)

    Nicoleta Moraru

    2013-05-01

    Full Text Available Chicory (Cichorium intybus L. has a series of important quality features due to both its human food value and to its animal feed value. As animal feed, the nutrient value of chicory is give mainly by its high content of organic substances which makes it a competitor of perennial fodder legumes. The paper presents the impact if fertilisation with chemical fertilisers (N50-100 and with manure (20-40 t/ha on dry matter production in chicory in the first vegetation year.

  4. Chicory (Cichorium intybus L.) Root Extract Regulates the Oxidative Status and Antioxidant Gene Transcripts in CCl4-Induced Hepatotoxicity

    OpenAIRE

    El-Sayed, Yasser S.; Lebda, Mohamed A.; Mohammed Hassinin; Neoman, Saad A.

    2015-01-01

    The ability of Cichorium intybus root extract (chicory extract) to protect against carbon tetrachloride (CCl4)-induced oxidative stress and hepatotoxicity was evaluated in male rats. The rats were divided into four groups according to treatment: saline (control); chicory extract (100 mg/kg body weight daily, given orally for 2 weeks); CCl4 (1 ml/kg body weight by intraperitoneal injection for 2 consecutive days only); or chicory extract (100 mg/kg body weight daily for 2 weeks) + CCl4 injecti...

  5. Effect of Chemical and Organic Fertilisation on Common Chicory (Cichorium intybus L.) in the First Vegetation Year

    OpenAIRE

    Nicoleta Moraru; Neculai Dragomir; Ioan Pădeanu; Carmen Dragomir; Fănel Tarjoc; Dorin Rechiţean

    2013-01-01

    Chicory (Cichorium intybus L.) has a series of important quality features due to both its human food value and to its animal feed value. As animal feed, the nutrient value of chicory is give mainly by its high content of organic substances which makes it a competitor of perennial fodder legumes. The paper presents the impact if fertilisation with chemical fertilisers (N50-100) and with manure (20-40 t/ha) on dry matter production in chicory in the first vegetation year.

  6. Chicory (Cichorium intybus L.) and cereals differently affect gut development in broiler chickens and young pigs.

    Science.gov (United States)

    Liu, Haoyu; Ivarsson, Emma; Lundh, Torbjörn; Lindberg, Jan Erik

    2013-01-01

    Dietary fiber, resistant to host-mediated digestion in the small intestine due to lack of endogenous enzymes, impacts many facets of animal health and is associated with gut development especially in young monogastrics. Furthermore, it can be used as in-feed antibiotic alternative. Chicory (Cichorium intybus L.) forage with high content of pectin (uronic acids as building blocks) is a novel class of dietary fiber that is chemically different from cereal grains (with high content of arabinoxylans). In the present study, we investigated effects of dietary inclusion of chicory forage on digestibility, gut morphology and microbiota in broilers and young pigs. In the chicken experiment, 160 1-d old broiler chicks were fed 3 nutritionally balanced diets for 30 d including a cereal-based diet and 2 diets with part of the cereals substituted with 60 and 120 g/kg chicory forage (CF60 and CF120), whereas in the pig experiment, 18 seven-wk old Yorkshire pigs were fed 3 diets for 18 d including a cereal-based diet and 2 diets with 80 and 160 g/kg chicory forage inclusion (CF80 and CF160). Our results showed that young pigs were capable to utilize chicory forage well with higher total tract apparent digestibility (TTAD) of all fiber fractions, particularly uronic acid, compared with the control (P microbiota revealed substantial dietary effects (cereal control diet vs. chicory forage inclusion) on the relative abundance of 2 dominant bacterial phylotypes (Prevotella sp. vs. Roseburia sp.) respectively (P ingredient in diets for both pigs and chickens. PMID:24341997

  7. Fatty acid composition of leaves of forced chicory (Cichorium intybus L.

    Directory of Open Access Journals (Sweden)

    Sinkovič Lovro

    2015-01-01

    Full Text Available The objective of the present study was to determine the composition of fatty acids in leaves of nine chicory cultivars (Cichorium intybus L.. The growing practice followed the traditional forcing method of developed roots in a peat to obtain new etiolated vegetative apical buds, known as chicons. The fatty acid content was determined by the extraction of fatty acid methyl esters and analysis by means of gas chromatography. The analysis revealed the following ratios of C16:0, C18:0, C18:1, C18:2 and C18:3 of individual fatty acids. The total fatty acid content in forced chicory leaves ranged from 104 to 644 mg/100 g fresh weight. The highest relative content (64% is presented by α-linolenic acid, followed by linoleic (44% and palmitic (21%. An n-6/n-3 polyunsaturated fatty acids ratio of studied forced chicory is below 1.4 and thus, in accordance with the recommended dietary ratio that is close to 1.

  8. YIELD AND QUALITY OF CHICORY (CICHORIUM INTYBUS L.) IN DEPENDENCE ON VARIETY AND FOLIAR APPLICATION OF ATONIK AND POLYBOR 150

    OpenAIRE

    IVAN ČERNÝ; VLADIMÍR PAČUTA; MAREK KOVÁR

    2009-01-01

    The influence of variety and foliar applications of Atonik and Polybor 150 on root yield and inulin content in root of chicory (Cichorium Intybus L.) plant were observed in field polyfactorial experiment. The field trials were established on experimental station Dolná Malanta in warm maize production area in 2005 and 2006 years. The root yield and inulin content were statistically high significantly influenced by year’s weather conditions. In term of obtained root yield and inulin content in ...

  9. YIELD AND QUALITY OF CHICORY (CICHORIUM INTYBUS L.) IN DEPENDENCE ON VARIETY AND FOLIAR APPLICATION OF ATONIK AND POLYBOR 150

    OpenAIRE

    ČERNÝ, IVAN; PAČUTA, VLADIMÍR; KOVÁR, MAREK

    2008-01-01

    The influence of variety and foliar applications of Atonik and Polybor 150 on root yield and inulin content in root of chicory (Cichorium Intybus L.) plant were observed in field polyfactorial experiment. The field trials were established on experimental station Dolná Malanta in warm maize production area in 2005 and 2006 years. The root yield and inulin content were statistically high significantly influenced by year’s weather conditions. In term of obtained root yield and inulin content in ...

  10. High temperatures limit plant growth but hasten flowering in root chicory (Cichorium intybus) independently of vernalisation.

    Science.gov (United States)

    Mathieu, Anne-Sophie; Lutts, Stanley; Vandoorne, Bertrand; Descamps, Christophe; Périlleux, Claire; Dielen, Vincent; Van Herck, Jean-Claude; Quinet, Muriel

    2014-01-15

    An increase in mean and extreme summer temperatures is expected as a consequence of climate changes and this might have an impact on plant development in numerous species. Root chicory (Cichorium intybus L.) is a major crop in northern Europe, and it is cultivated as a source of inulin. This polysaccharide is stored in the tap root during the first growing season when the plant grows as a leafy rosette, whereas bolting and flowering occur in the second year after winter vernalisation. The impact of heat stress on plant phenology, water status, photosynthesis-related parameters, and inulin content was studied in the field and under controlled phytotron conditions. In the field, plants of the Crescendo cultivar were cultivated under a closed plastic-panelled greenhouse to investigate heat-stress conditions, while the control plants were shielded with a similar, but open, structure. In the phytotrons, the Crescendo and Fredonia cultivars were exposed to high temperatures (35°C day/28°C night) and compared to control conditions (17°C) over 10 weeks. In the field, heat reduced the root weight, the inulin content of the root and its degree of polymerisation in non-bolting plants. Flowering was observed in 12% of the heat stressed plants during the first growing season in the field. In the phytotron, the heat stress increased the total number of leaves per plant, but reduced the mean leaf area. Photosynthesis efficiency was increased in these plants, whereas osmotic potential was decreased. High temperature was also found to induced flowering of up to 50% of these plants, especially for the Fredonia cultivar. In conclusion, high temperatures induced a reduction in the growth of root chicory, although photosynthesis is not affected. Flowering was also induced, which indicates that high temperatures can partly substitute for the vernalisation requirement for the flowering of root chicory. PMID:24331425

  11. Sink filling, inulin metabolizing enzymes and carbohydrate status in field grown chicory (Cichorium intybus L.).

    Science.gov (United States)

    van Arkel, Jeroen; Vergauwen, Rudy; Sévenier, Robert; Hakkert, Johanna C; van Laere, André; Bouwmeester, Harro J; Koops, Andries J; van der Meer, Ingrid M

    2012-10-15

    Inulin is a fructose-based polymer that is isolated from chicory (Cichorium intybus L.) taproots. The degree of polymerization (DP) determines its application and hence the value of the crop. The DP is highly dependent on the field conditions and harvest time. Therefore, the present study was carried out with the objective to understand the regulation of inulin metabolism and the process that determines the chain length and inulin yield throughout the whole growing season. Metabolic aspects of inulin production and degradation in chicory were monitored in the field and under controlled conditions. The following characteristics were determined in taproots: concentrations of glucose, fructose and sucrose, the inulin mean polymer length (mDP), yield, gene expression and activity of enzymes involved in inulin metabolism. Inulin synthesis, catalyzed by sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99) (1-SST) and fructan:fructan 1-fructosyltransferase (EC 2.4.1.100) (1-FFT), started at the onset of taproot development. Inulin yield as a function of time followed a sigmoid curve reaching a maximum in November. Inulin reached a maximum mDP of about 15 in September, than gradually decreased. Based on the changes observed in the pattern of inulin accumulation, we defined three different phases in the growing season and analyzed product formation, enzyme activity and gene expression in these defined periods. The results were validated by performing experiments under controlled conditions in climate rooms. Our results show that the decrease in 1-SST that starts in June is not regulated by day length and temperature. From mid-September onwards, the mean degree of polymerization (mDP) decreased gradually although inulin yield still increased. The decrease in mDP combined with increased yield results from fructan exohydrolase activity, induced by low temperature, and the back transfer activity of 1-FFT. Overall, this study provides background information on how to improve

  12. Identification of novel genes potentially involved in somatic embryogenesis in chicory (Cichorium intybus L.

    Directory of Open Access Journals (Sweden)

    Quillet Marie-Christine

    2010-06-01

    Full Text Available Abstract Background In our laboratory we use cultured chicory (Cichorium intybus explants as a model to investigate cell reactivation and somatic embryogenesis and have produced 2 chicory genotypes (K59, C15 sharing a similar genetic background. K59 is a responsive genotype (embryogenic capable of undergoing complete cell reactivation i.e. cell de- and re-differentiation leading to somatic embryogenesis (SE, whereas C15 is a non-responsive genotype (non-embryogenic and is unable to undergo SE. Previous studies 1 showed that the use of the β-D-glucosyl Yariv reagent (β-GlcY that specifically binds arabinogalactan-proteins (AGPs blocked somatic embryo production in chicory root explants. This observation indicates that β-GlcY is a useful tool for investigating somatic embryogenesis (SE in chicory. In addition, a putative AGP (DT212818 encoding gene was previously found to be significantly up-regulated in the embryogenic K59 chicory genotype as compared to the non-embryogenic C15 genotype suggesting that this AGP could be involved in chicory re-differentiation 2. In order to improve our understanding of the molecular and cellular regulation underlying SE in chicory, we undertook a detailed cytological study of cell reactivation events in K59 and C15 genotypes, and used microarray profiling to compare gene expression in these 2 genotypes. In addition we also used β-GlcY to block SE in order to identify genes potentially involved in this process. Results Microscopy confirmed that only the K59, but not the C15 genotype underwent complete cell reactivation leading to SE formation. β-GlcY-treatment of explants blocked in vitro SE induction, but not cell reactivation, and induced cell wall modifications. Microarray analyses revealed that 78 genes were differentially expressed between induced K59 and C15 genotypes. The expression profiles of 19 genes were modified by β-GlcY-treatment. Eight genes were both differentially expressed between K59 and C

  13. Effects of the extract from roasted chicory (Cichorium intybus L.) root containing inulin-type fructans on blood glucose, lipid metabolism, and fecal properties

    OpenAIRE

    Nishimura, Mie; Ohkawara, Tatsuya; Kanayama, Toshiyuki; Kitagawa, Kazuya; Nishimura, Hiroyuki; Nishihira, Jun

    2015-01-01

    The extract from roasted chicory (Cichorium intybus L.; 菊苣 jú jù) root (chicory root extract), which contains inulin-type fructans, has favorable effects including antihyperglycemic and antidyslipidemic effects and the improvement of bowel movement. In this study, we examined the effects of chicory root extract on blood glucose, lipid metabolism, and fecal properties in 47 healthy adult participants in a randomized, double-blind, placebo-controlled study. The participants were divided into a ...

  14. Comparison of the Phenolics Profiles of Forced and Unforced Chicory (Cichorium intybus L. Cultivars

    Directory of Open Access Journals (Sweden)

    Lovro Sinkovič

    2014-10-01

    Full Text Available Modern and sustainable production of safe and healthy high quality vegetables for human consumption is nowadays the goal of many producers. Chicory (Cichorium intybus L. has become an important crop used as food in many countries over the past decade. In this work field and glasshouse trials were conducted to compare phenolic profiles of hydroponically forced leaves and leaves produced as common agronomic practice of five chicory cultivars. Total phenolic content of hydroponically forced samples ranged from 60 to 140 mg/100 g fresh weight, and those of unforced grown leaves varied between 117 and 386 mg/100 g fresh weight. Red cultivar ‘Treviso’ shows the highest phenolic content for both, unforced and forced leaves extracts. In the unforced leaves a total of 33 and in those of forced a total of 44 compounds (peaks were used for the discrimination study. Normal 0 21 false false false SL X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Navadna tabela"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-fareast-language:EN-US;}

  15. Chicory (Cichorium intybus L.) root extract regulates the oxidative status and antioxidant gene transcripts in CCl4-induced hepatotoxicity.

    Science.gov (United States)

    El-Sayed, Yasser S; Lebda, Mohamed A; Hassinin, Mohammed; Neoman, Saad A

    2015-01-01

    The ability of Cichorium intybus root extract (chicory extract) to protect against carbon tetrachloride (CCl4)-induced oxidative stress and hepatotoxicity was evaluated in male rats. The rats were divided into four groups according to treatment: saline (control); chicory extract (100 mg/kg body weight daily, given orally for 2 weeks); CCl4 (1 ml/kg body weight by intraperitoneal injection for 2 consecutive days only); or chicory extract (100 mg/kg body weight daily for 2 weeks) + CCl4 injection on days 16 and 17. The levels of hepatic lipid peroxidation, antioxidants, and molecular biomarkers were estimated twenty-four hours after the last CCl4 injection. Pretreatment with chicory extract significantly reduced CCl4-induced elevation of malondialdehyde levels and nearly normalized levels of glutathione and activity of glutathione S-transferase, glutathione peroxidase (GPx), glutathione reductase, catalase (CAT), paraoxonase-1 (PON1), and arylesterase in the liver. Chicory extract also attenuated CCl4-induced downregulation of hepatic mRNA expression levels of GPx1, CAT and PON1 genes. Results of DNA fragmentation support the ability of chicory extract to ameliorate CCl4-induced liver toxicity. Taken together, our results demonstrate that chicory extract is rich in natural antioxidants and able to attenuate CCl4-induced hepatocellular injury, likely by scavenging reactive free radicals, boosting the endogenous antioxidant defense system, and overexpressing genes encoding antioxidant enzymes. PMID:25807561

  16. Chicory (Cichorium intybus L. root extract regulates the oxidative status and antioxidant gene transcripts in CCl4-induced hepatotoxicity.

    Directory of Open Access Journals (Sweden)

    Yasser S El-Sayed

    Full Text Available The ability of Cichorium intybus root extract (chicory extract to protect against carbon tetrachloride (CCl4-induced oxidative stress and hepatotoxicity was evaluated in male rats. The rats were divided into four groups according to treatment: saline (control; chicory extract (100 mg/kg body weight daily, given orally for 2 weeks; CCl4 (1 ml/kg body weight by intraperitoneal injection for 2 consecutive days only; or chicory extract (100 mg/kg body weight daily for 2 weeks + CCl4 injection on days 16 and 17. The levels of hepatic lipid peroxidation, antioxidants, and molecular biomarkers were estimated twenty-four hours after the last CCl4 injection. Pretreatment with chicory extract significantly reduced CCl4-induced elevation of malondialdehyde levels and nearly normalized levels of glutathione and activity of glutathione S-transferase, glutathione peroxidase (GPx, glutathione reductase, catalase (CAT, paraoxonase-1 (PON1, and arylesterase in the liver. Chicory extract also attenuated CCl4-induced downregulation of hepatic mRNA expression levels of GPx1, CAT and PON1 genes. Results of DNA fragmentation support the ability of chicory extract to ameliorate CCl4-induced liver toxicity. Taken together, our results demonstrate that chicory extract is rich in natural antioxidants and able to attenuate CCl4-induced hepatocellular injury, likely by scavenging reactive free radicals, boosting the endogenous antioxidant defense system, and overexpressing genes encoding antioxidant enzymes.

  17. Atmospheric cold plasma process for vegetable leaf decontamination: A feasibility study on radicchio (red chicory, Cichorium intybus L.)

    OpenAIRE

    Pasquali, Frederique; Stratakos, Alexandros Ch; Koidis, Anastasios; Berardinelli, Annachiara; Cevoli, Chiara; Ragni, Luigi; Mancusi, Rocco; Manfreda, Gerardo; Trevisani, Marcello

    2016-01-01

    Cold plasma is an emerging non-thermal processing technology that could be used for large scale leaf decontamination as an alternative to chlorine washing. In this study the effect of an atmospheric cold plasma apparatus (air DBD, 15 kV) on the safety, antioxidant activity and quality of radicchio (red chicory, Cichorium intybus L.) was investigated after 15 and 30 min of treatment (in afterglow at 70 mm from the discharge, at 22 °C and 60% of RH) and during storage. Escherichia coli O157:H7 ...

  18. Antioxidants in Varieties of Chicory (Cichorium intybus L. and Wild Poppy (Papaver rhoeas L. of Southern Italy

    Directory of Open Access Journals (Sweden)

    Anna Montefusco

    2015-01-01

    Full Text Available We report the hydrophilic and lipophilic antioxidant activities, as well as the total phenol, flavonoid, tocochromanol (tocopherol and tocotrienol, and carotenoid contents in the edible portion of wild and cultivated varieties of chicory (Cichorium intybus L. and in the basal rosette leaves of the wild species of poppy (Papaver rhoeas L., known by natives as “paparina,” collected in the countryside of Salento (South Apulia, Italy. We analyzed (1 two cultivars of chicory, the “Catalogna” harvested in the area between S. Pietro Vernotico and Tuturano (Brindisi and the “Otrantina” harvested in Otranto (Lecce; (2 two wild chicory ecotypes harvested in S. Pietro Vernotico (Brindisi and Statte (Taranto, respectively; (3 the basal leaves of wild poppy harvested in Sternatia (Lecce. In all samples, our results showed that the hydrophilic antioxidant activity is, generally, higher than the lipophilic activity. Poppy leaves exhibited the highest hydrophilic and lipophilic antioxidant activities and the highest concentration of total phenols and flavonoids. Tocopherols were detected only as traces. Among the extracted carotenoids, lutein and β-carotene were the most abundant in all analyzed samples. Total carotenoid content was greater in wild than in cultivated plants.

  19. Phenolic profiles in leaves of chicory cultivars (Cichorium intybus L.) as influenced by organic and mineral fertilizers.

    Science.gov (United States)

    Sinkovič, Lovro; Demšar, Lea; Žnidarčič, Dragan; Vidrih, Rajko; Hribar, Janez; Treutter, Dieter

    2015-01-01

    Chicory (Cichorium intybus L.) is a typical Mediterranean vegetable, and it shows great morphological diversity, including different leaf colours. Five cultivars commonly produced in Slovenia ('Treviso', 'Verona', 'Anivip', 'Castelfranco', 'Monivip') were grown in pots under controlled conditions in a glasshouse, with organic and/or mineral fertilizers administered to meet nitrogen requirements. HPLC analysis was carried out to study the phenolic compositions of the leaves. A total of 33 phenolic compounds were extracted from these chicory leaves and were quantitatively evaluated in an HPLC-DAD-based metabolomics study. Among the cultivars, the highest TPC was seen for 'Treviso' (300.1 mg/100 g FW), and the lowest, for 'Castelfranco' (124.9 mg/100g FW). Across the different treatments, the highest TPC was in the control samples (254.3 mg/100 g FW), and the lowest for the organic (128.6 mg/100 g FW) and mineral fertilizer (125.5 mg/100 g FW) treatments. The predominant phenolic compounds in all of the samples were hydroxycinnamic acids, including chlorogenic and cichoric acid. Fertilizer administration provides a discriminant classification of the chicory cultivars according to their phenolic compounds. PMID:25053087

  20. Regulation of cytochrome P450 mRNA expression in primary porcine hepatocytes by selected secondary plant metabolites from chicory (Cichorium intybus L.)

    OpenAIRE

    Rasmussen, Martin Krøyer; Klausen, Christina Lindgaard; Ekstrand, Bo

    2013-01-01

    Chicory (Cichorium intybus) has been shown to induce enzymes of pharmacokinetic relevance (cytochrome P450; CYP). The aim of this study was to investigate the effects of selected secondary plant metabolites with a global extract of chicory root, on the expression of hepatic CYP mRNA (1A2, 2A19, 2C33, 2D25, 2E1 and 3A29), using primary porcine hepatocytes. Of the tested secondary plant metabolites, artemisinin, scoparone, lactucin and esculetin all induced increased expression of specific CYPs...

  1. Regulation of cytochrome P450 mRNA expression in primary porcine hepatocytes by selected secondary plant metabolites from chicory (Cichorium intybus L.).

    Science.gov (United States)

    Rasmussen, Martin Krøyer; Klausen, Christina Lindgaard; Ekstrand, Bo

    2014-03-01

    Chicory (Cichorium intybus) has been shown to induce enzymes of pharmacokinetic relevance (cytochrome P450; CYP). The aim of this study was to investigate the effects of selected secondary plant metabolites with a global extract of chicory root, on the expression of hepatic CYP mRNA (1A2, 2A19, 2C33, 2D25, 2E1 and 3A29), using primary porcine hepatocytes. Of the tested secondary plant metabolites, artemisinin, scoparone, lactucin and esculetin all induced increased expression of specific CYPs, while esculin showed no effect. In contrast, a global extract of chicory root decreased the expression of CYP1A2, 2C33, 2D25 and 3A29 at high concentrations. The results suggest that purified secondary metabolites from chicory affect CYP expression and thereby might affect detoxification in general, and that global extracts of plants can have effects different from individual components. PMID:24176340

  2. Effects of the extract from roasted chicory (Cichorium intybus L.) root containing inulin-type fructans on blood glucose, lipid metabolism, and fecal properties.

    Science.gov (United States)

    Nishimura, Mie; Ohkawara, Tatsuya; Kanayama, Toshiyuki; Kitagawa, Kazuya; Nishimura, Hiroyuki; Nishihira, Jun

    2015-07-01

    The extract from roasted chicory (Cichorium intybus L.; jú jù) root (chicory root extract), which contains inulin-type fructans, has favorable effects including antihyperglycemic and antidyslipidemic effects and the improvement of bowel movement. In this study, we examined the effects of chicory root extract on blood glucose, lipid metabolism, and fecal properties in 47 healthy adult participants in a randomized, double-blind, placebo-controlled study. The participants were divided into a test group that drank chicory root extract and a placebo group that drank nonchicory root extract (ingesting 300 mL daily for 4 weeks). We performed hematological examinations and body composition measurements, and administered a visual analog scale (VAS) questionnaire for fecal properties at the baseline (Week 0) and after the intervention (Week 4) for the two groups. Although no significant differences in fasting plasma glucose or insulin were observed, hemoglobin A1c was found to decrease by ingesting chicory root extract. No intergroup differences in the levels of lipid metabolism parameters were observed. However, the level of adiponectin was significantly improved in the chicory root extract group when the baseline and postintervention values were compared. In addition, chicory root extract tends to improve the VAS score for fecal properties. These results suggest that chicory root extract could delay or prevent the early onset of diabetes mellitus and improve bowel movements. PMID:26151029

  3. YIELD AND QUALITY OF CHICORY (CICHORIUM INTYBUS L. IN DEPENDENCE ON VARIETY AND FOLIAR APPLICATION OF ATONIK AND POLYBOR 150

    Directory of Open Access Journals (Sweden)

    IVAN ČERNÝ

    2009-03-01

    Full Text Available The influence of variety and foliar applications of Atonik and Polybor 150 on root yield and inulin content in root of chicory (Cichorium Intybus L. plant were observed in field polyfactorial experiment. The field trials were established on experimental station Dolná Malanta in warm maize production area in 2005 and 2006 years. The root yield and inulin content were statistically high significantly influenced by year’s weather conditions. In term of obtained root yield and inulin content in root the combination of foliar preparations on variant B (Atonik: 0.4 l ha-1 in 2nd post-emergence herbicide application; Atonik + Polybor 150: 0.6 + 2.5 l ha-1 in 3rd post-emergence herbicide application; Polybor 150: 2.5 l ha-1 in 1st fungicide treatment was shown to be optimal in given agri-ecological conditions. Biological material affected the formation of yield parameters very differently. The highest yield of root was observed at variety Fredonia Nova and inulin content in root at variety Maurane (both statistically significant.

  4. Selenium uptake and distribution in chicory (Chichorium intybus L.) grown in an aeroponic system: Privzem in porazdelitev selena v radiču (cichorium intybus L.), gojenem v aeroponskem sistemu:

    OpenAIRE

    Jaćimović, Radojko; Osvald, Jože; Smrkolj, Polona; Stibilj, Vekoslava

    2011-01-01

    Cultivated plants generally are a poor source of dietary selenium (< 0.1 mgžkg wet weight). In this work the influence of sodium selenate on selenium distribution in the chicory (Cichorium intybus L.) cultivars ćAnivipć and ćMonivipć was studied in an aeroponic system in a greenhouse. The plants rootswere moistened every fifteen minutes with a Resh nutrient solution with added selenium (10 mg L-1) in the form of sodium selenate. The content of total selenium in chicory roots and leaves was st...

  5. The effect of Cichorium intybus on helminth infections in pigs

    OpenAIRE

    Mejer, Helena; Roepstorff, Allan; Thamsborg, Stig Milan; Lydehøj Hansen, Laurits; Bach Knudsen, Knud Erik

    2005-01-01

    Two similar trials were carried out to examine how dietary inclusion of an easily fermentable carbohydrate source such as chicory roots (Cichorium intybus) may effect established and establishing helminth infections in pigs. In the first trial varying feeding periods with crude chicory roots naturally rich in fructans (primarily inulin) were tested whereas crude chicory roots were compared with dried chicory roots and purified inulin in the second trial. In both trials pigs were infected with...

  6. Cichorium intybus: Traditional Uses, Phytochemistry, Pharmacology, and Toxicology

    OpenAIRE

    Renée A. Street; Jasmeen Sidana; Gerhard Prinsloo

    2013-01-01

    The genus Cichorium (Asteraceae) is made up of six species with major geographical presence in Europe and Asia. Cichorium intybus, commonly known as chicory, is well known as a coffee substitute but is also widely used medicinally to treat various ailments ranging from wounds to diabetes. Although this plant has a rich history of use in folklore, many of its constituents have not been explored for their pharmacological potential. Toxicological data on C. intybus is currently limited. This rev...

  7. Dry matter yield, feeding value, and antioxidant activity in Mediterranean chicory (Cichorium intybus L.) germplasm

    OpenAIRE

    PILUZZA, Giovanna; SULAS, Leonardo; BULLITTA, Simonetta

    2014-01-01

    Knowledge about the forage yield potential and bioactive compound contents of Mediterranean natural populations of chicory is still scarce, even though it could be very helpful in view of the exploitation of the feeding value of local chicory germplasm for ruminants. Dry matter yield, chemical composition, antioxidant capacity, total polyphenols, flavonoids, and condensed tannins content were determined in 7 natural populations of Mediterranean wild chicory and, as a comparison, in the chicor...

  8. Construction of 12 EST libraries and characterization of a 12,226 EST dataset for chicory (Cichorium intybus) root, leaves and nodules in the context of carbohydrate metabolism investigation

    OpenAIRE

    Boutry Marc; Watillon Bernard; Purnelle Bénédicte; Muys Céline; Mingeot Dominique; Dauchot Nicolas; Van Cutsem Pierre

    2009-01-01

    Abstract Background The industrial chicory, Cichorium intybus, is a member of the Asteraceae family that accumulates fructan of the inulin type in its root. Inulin is a low calories sweetener, a texture agent and a health promoting ingredient due to its prebiotic properties. Average inulin chain length is a critical parameter that is genotype and temperature dependent. In the context of the study of carbohydrate metabolism and to get insight into the transcriptome of chicory root and to visua...

  9. Anthelmintic effects of forage chicory (Cichorium intybus) against gastrointestinal nematode parasites in experimentally infected cattle

    OpenAIRE

    Peña-Espinoza, Miguel; Thamsborg, Stig Milan; Desrues, Olivier; Hansen, T. V. A.; Enemark, Heidi L.

    2016-01-01

    Two experiments studied the effects of dietary chicory against gastrointestinal nematodes in cattle. In Experiment (Exp.) 1, stabled calves were fed chicory silage (CHI1; n = 9) or ryegrass/clover hay (CTL1; n = 6) with balanced protein/energy intakes between groups. After 16 days, all calves received 10 000 Ostertagia ostertagi and 66 000 Cooperia oncophora third-stage larvae (L3) [day (D) 0 post-infection (p.i.)]. In Exp. 2, calves were assigned to pure chicory (CHI2; n=10) or ryegrass/clov...

  10. Feeding chicory ( Cichorium intybus ) selectively reduces Ostertagia ostertagi infection levels in cattle

    OpenAIRE

    Pena-Espinoza, Miguel Angel; Desrues, Oliver; Hansen, Tina; Thamsborg, Stig M.; Enemark, Heidi

    2015-01-01

    Objectives: Studies were conducted to test the potential use of chicory against gastrointestinal nematode infections in cattle. Methods: In study 1, fifteen 2-4 months-old dairy calves were allocated into a chicory (CHI, n=9) or control (CTL, n=6) group. CHI and CTL were stabled and fed with chicory silage or hay, resp., ad lib for 56 days. Protein/energy intakes were equalized between groups throughout the study. After 14 days on the diet all calves were infected with 10,000 Ostertagia oster...

  11. Chicory (Cichorium intybus L.) as bio-indicator of heavy metal contamination

    Energy Technology Data Exchange (ETDEWEB)

    Simon, L. [University of Agricultural Sciences, Nyiregyhaza, (Hungary). College of Agriculture, Faculty of the Godollo; Prokisch, J.; Kovacs, B. [Debrecen University of Agricultural Sciences (Hungary). Central Chemical Laboratory

    1997-12-31

    Greenhouse pot experiments were conducted with chicory; plants were grown in a sandy-loam brown forest soil medium amended with increasing amounts of municipal sewage aerobically composted with wheat straw. The sewage sludge compost contained higher concentrations of total and plant available Cd, Cr, Cu, Pb and Zn, and less Co and Mn than the basic soil. Underground parts (roots and rhizomes) and top parts (shoots) of chicory accumulated Cd, Cr, Cu, Mn and Zn from the soil-sewage sludge compost. Linearly increasing amounts of Zn, Mn and Cd with increasing amounts of applied compost, were found in roots and rhizomes and in shoots of chicory; chicory is thus proposed as an indicator plant of Zn, Mn and Cd contamination in sewage sludge compost

  12. Effects of Nuclear Genomes on Anther Development in Cytoplasmic Male Sterile Chicories (Cichorium intybus L.): Morphological Analysis

    OpenAIRE

    Ildephonse Habarugira; Theo Hendriks; Marie-Christine Quillet; Jean-Louis Hilbert; Caroline Rambaud

    2015-01-01

    The Cichorium intybus flower development in fertile, cytoplasmic male sterility (CMS 524) and various phenotypes carrying the 524 male sterile cytoplasm was investigated macroscopically and by light microscopy. The development was similar in fertile and in male sterile florets up to meiosis, and then it was affected in anther wall structure and pollen grain development in male sterile floret. In the male sterile plants, the tapetum intrusion after meiosis was less remarkable, the microspores...

  13. Differential expression of fructan 1-exohydrolase genes involved in inulin biodegradation in chicory (Cichorium intybus) cultivars

    OpenAIRE

    Maroufi, Asaf; Van Bockstaele, Erik; De Loose, Marc

    2012-01-01

    Fructan 1-exohydrolase (1-FEH; FEH) enzymes are involved in inulin degradation in the roots of chicory. rehgiH FEH expression in cold temperatures can decrease the quality and the quantity of the inulin. This is the case at the end of the growing season and during cold storage. Little is known at molecular level whether the expression levels of fructan 1-exohydrolase genes vary among chicory cultivars, especially during cold storage of roots. Real-time RT-PCR is the most sensitive method for ...

  14. Genealogy, morphology and fitness of spontaneous hybrids between wild and cultivated chicory (Cichorium intybus)

    DEFF Research Database (Denmark)

    Kiær, Lars Pødenphant; Philipp, M.; Bagger Jørgensen, Rikke;

    2007-01-01

    of cultivar-like and intermediate plants preferentially. Thus, cultivated and wild chicory are able to hybridize spontaneously, producing hybrid offspring of several generations that may reproduce more effectively than their wild parent, but herbivory and poor environmental conditions may negatively...

  15. Hydrioxylation of sesquiterpenes by enzymes from chicory (Cichorium intybus L.) roots

    NARCIS (Netherlands)

    Kraker, de J.W.; Schurink, M.; Franssen, M.C.R.; König, W.A.; Groot, de Æ.; Bouwmeester, H.J.

    2003-01-01

    A microsomal enzyme preparation of chicory roots catalyses the hydroxylation of various sesquiterpene olefins in the presence of NADPH. Most of these hydroxylations take place at an isopropenyl or isopropylidene group. The number of products obtained from any of the substrates is confined to one or,

  16. In Silico Analysis and Experimental Validation of Active Compounds from Cichorium intybus L. Ameliorating Liver Injury

    OpenAIRE

    Guo-Yu Li; Ya-Xin Zheng; Fu-Zhou Sun; Jian Huang; Meng-Meng Lou; Jing-Kai Gu; Jin-Hui Wang

    2015-01-01

    This study aimed at investigating the possible mechanisms of hepatic protective activity of Cichorium intybus L. (chicory) in acute liver injury. Pathological observation, reactive oxygen species (ROS) detection and measurements of biochemical indexes on mouse models proved hepatic protective effect of Cichorium intybus L. Identification of active compounds in Cichorium intybus L. was executed through several methods including ultra performance liquid chromatography/time of flight mass spectr...

  17. The influence of intercrop plants and the date of their ploughing-in on weed infestation of root chicory (Cichorium intybus L. var. sativum (Bisch. Janch.

    Directory of Open Access Journals (Sweden)

    Marzena Błażewicz-Woźniak

    2012-12-01

    Full Text Available The field experiment was carried out in 2006-2008 in the Felin Experimental Farm (University of Life Sciences in Lublin on podzolic soil developed from dusty medium loam. Root chicory (Cichorium intybus L. var. sativum (Bisch. Janch. cv. Polanowicka was involved in the experiment. The experimental factors were 3 species of intercrop plants: common vetch (Vicia sativa, phacelia (Phacelia tanacetifolia, oat (Avena sativa and 2 dates of ploughing-in: pre-winter and spring. In total, 26 taxons characteristic for vegetable plantations were identified in chicory weed infestation. Monocarpic species dominated, among which Senecio vulgaris, Chenopodium album, Lamium amplexicaule, Galinsoga ciliata, and Capsella bursa-pastoris were predominant. The date of ploughing-in did not significantly affect the status and size of weed infestation of chicory plots. Short-lived species occurred after pre-winter ploughing-in, while perennial - after spring ploughing-in. The application of intercrops significantly reduced chicory weed infestation as compared to the cultivation with no intercrop. The ploughing-in of Avena sativa biomass appeared to be the most efficient. The intercrop plants reduced the occurrence of Senecio vulgaris and Capsella bursa-pastoris which were the most numerous in the treatment without intercrops. Biomass of Vicia sativa favored the growth of Chenopodium album and Lamium amplexicaule. The secondary weed infestation did not depend on agrotechnical factors applied during the experiment.

  18. Cichorium intybus: Traditional Uses, Phytochemistry, Pharmacology, and Toxicology.

    Science.gov (United States)

    Street, Renée A; Sidana, Jasmeen; Prinsloo, Gerhard

    2013-01-01

    The genus Cichorium (Asteraceae) is made up of six species with major geographical presence in Europe and Asia. Cichorium intybus, commonly known as chicory, is well known as a coffee substitute but is also widely used medicinally to treat various ailments ranging from wounds to diabetes. Although this plant has a rich history of use in folklore, many of its constituents have not been explored for their pharmacological potential. Toxicological data on C. intybus is currently limited. This review focuses on the economic and culturally important medicinal uses of C. intybus. Traditional uses, scientific validation, and phytochemical composition are discussed in detail. PMID:24379887

  19. A “Novel” Protocol for the Analysis of Hydroxycinnamic Acids in Leaf Tissue of Chicory (Cichorium intybus L., Asteraceae)

    OpenAIRE

    Meriem Bahri; Philippe Hance; Sébastien Grec; Marie-Christine Quillet; Francis Trotin; Jean-Louis Hilbert; Theo Hendriks

    2012-01-01

    A “novel” protocol is presented for easy and reliable estimation of soluble hydroxycinnamate levels in Cichorium intybus L. leaf tissue in large-scale experiments. Samples were standardized by punching 6 discs per leaf, and hydroxycinnamates were extracted by submerging the discs in 80% ethanol with 5% acetic acid for at least 48 h in the darkness at 4°C. Residual dry mass of the discs was used for a posteriori correction of compound levels. Chlorophyll was eliminated by chloroform, and the a...

  20. Agrobacterium mediated transfer of a mutant Arabidopsis acetolactate synthase gene confers resistance to chlorsulfuron in chicory (Cichorium intybus L.).

    Science.gov (United States)

    Vermeulen, A; Vaucheret, H; Pautot, V; Chupeau, Y

    1992-06-01

    Leaf discs of C. intybus were inoculated with an Agrobacterium tumefaciens strain harboring a neomycin phosphotransferase (neo) gene for kanamycin resistance and a mutant acetolactate synthase gene (csr1-1) from Arabidopsis thaliana conferring resistance to sulfonylurea herbicides. A regeneration medium was optimized which permitted an efficient shoot regeneration from leaf discs. Transgenic shoots were selected on rooting medium containing 100 mg/l kanamycin sulfate. Integration of the csr1-1 gene into genomic DNA of kanamycin resistant chicory plants was confirmed by Southern blot hybridizations. Analysis of the selfed progenies (S1 and S2) of two independent transformed clones showed that kanamycin and chlorsulfuron resistances were inherited as dominant Mendelian traits. The method described here for producing transformed plants will allow new opportunities for chicory breeding. PMID:24203132

  1. Possible Ameliorative Effect of Chicory Extract (Cichorium Intybus) on Radiation-Induced Oxidative Damage in Rats Heart

    International Nuclear Information System (INIS)

    The radioprotective effect of aqueous leaf extract of Chicorium intybus (Chicory) against radiation induced-oxidative stress and changes in the levels of 150-180 g were divided into four groups. Group 1: control animals, group 2: animals orally administrated with chicory extract at a daily dose of 250 mg/kg b.wt/day for four weeks, group 3: animals exposed to whole body gamma irradiation (6.5 Gy), group 4: animals orally administrated with chicory extract two weeks before and two weeks after irradiation. Serum level of creatinine phosphokinase (CPK), lactate dehydrogenase (LDH), serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lipid profile was measured.also concentration of superoxide dismutase (SOD), glutathione (GSH), Catalase (CAT) and TBARS level was estimated in the cardiac tissue. The results showed decreased body weight and heart weight in irradiated animals. Compared to the control normal rats, irradiated rats had higher total cholesterol, triglycerides, low-density lipoprotein-cholesterol (LDL-C), serum creatinine phosphokinase(CPK), lactate dehydrogenase (LDH), serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lower high-density lipoprotein-cholesterol (HDL-C) levels. Moreover, cardiac tissue TBARS was markedly increased while SOD, GSH and CAT were significantly decreased. Oral and heart weights, serum cardiac enzymes and lipid profile. Cardiac GSH, SOD and CAT were significantly increased while TBARS was markedly reduced, membrane bound enzymes in rats' heart was investigated. Rats weighing about administration of chicory extract at doses of 250 mg/kg b.wt. improved the body compared to irradiated rats. These results may suggest a strong antioxidant effect of chicory, which was effective in mitigating adverse effect of γ irradiation on animals

  2. [Food value of cichorium intybus].

    Science.gov (United States)

    Luzina, E V

    2013-01-01

    Cichorium intybus is an herbaceous perennial, known as a coffee substitute. It possesses a wide range of healing characteristics particularly due to inulin, one of its components. Inulin is a natural polysaccharide, a polyfructosane containing 27-35 fructose residues in furanose form and glucose residue. When inulin is in the alimentary tract it passes to the stomach and the small intestine unchangeable. In large intestine inulin is fermented by bifidobacteria and then converted in to a great number of short-chain fatty acids. Due to the process it stimulated the growth of bifidobacteria population and reduces that of pathogenic microorganisms. The increase of pool of healthy intestinalflora normalizes the passage of fieces masses through the intestine, improves immunologic status, regulates carbohydrate and lipid metabolism. The paper presents experimental and clinic studies confirming bifidogenic, immunogenic, anticancerous, hepatoprotective characteristics of inulin and cichorium intybus. PMID:24000703

  3. Effects of nuclear genomes on anther development in cytoplasmic male sterile chicories (Cichorium intybus L.): morphological analysis.

    Science.gov (United States)

    Habarugira, Ildephonse; Hendriks, Theo; Quillet, Marie-Christine; Hilbert, Jean-Louis; Rambaud, Caroline

    2015-01-01

    The Cichorium intybus flower development in fertile, cytoplasmic male sterility (CMS 524) and various phenotypes carrying the 524 male sterile cytoplasm was investigated macroscopically and by light microscopy. The development was similar in fertile and in male sterile florets up to meiosis, and then it was affected in anther wall structure and pollen grain development in male sterile floret. In the male sterile plants, the tapetum intrusion after meiosis was less remarkable, the microspores started to abort at vacuolate stage, the connective tissue collapsed, and endothecium failed to expand normally and did not undergo cell wall lignification, which prevented anther opening since the septum and stomium were not disrupted. Crosses undertaken in order to introduce the CMS 524 into two different nuclear backgrounds gave rise to morphologically diversified progenies due to different nuclear-mitochondrial interactions. Macroscopic and cytological investigations showed that pollen-donor plants belonging to Jupiter population had potential capacity to restore fertility while the CC line could be considered as a sterility maintainer. PMID:25861678

  4. A “Novel” Protocol for the Analysis of Hydroxycinnamic Acids in Leaf Tissue of Chicory (Cichorium intybus L., Asteraceae

    Directory of Open Access Journals (Sweden)

    Meriem Bahri

    2012-01-01

    Full Text Available A “novel” protocol is presented for easy and reliable estimation of soluble hydroxycinnamate levels in Cichorium intybus L. leaf tissue in large-scale experiments. Samples were standardized by punching 6 discs per leaf, and hydroxycinnamates were extracted by submerging the discs in 80% ethanol with 5% acetic acid for at least 48 h in the darkness at 4°C. Residual dry mass of the discs was used for a posteriori correction of compound levels. Chlorophyll was eliminated by chloroform, and the aqueous phases were transferred to microplates, dried, and dissolved in 50% methanol for HPLC analysis and storage. An HPLC program of 8 min was developed for the analysis of the extracts. Comparisons with extractions of liquid nitrogen powders indicated that the novel extraction method was reliable. No degradation of the major hydroxycinnamates—caftaric, chlorogenic, and chicoric acids—was observed, during maceration at ambient temperatures, or after storage for 1 year.

  5. Effects of Nuclear Genomes on Anther Development in Cytoplasmic Male Sterile Chicories (Cichorium intybus L.: Morphological Analysis

    Directory of Open Access Journals (Sweden)

    Ildephonse Habarugira

    2015-01-01

    Full Text Available The Cichorium intybus flower development in fertile, cytoplasmic male sterility (CMS 524 and various phenotypes carrying the 524 male sterile cytoplasm was investigated macroscopically and by light microscopy. The development was similar in fertile and in male sterile florets up to meiosis, and then it was affected in anther wall structure and pollen grain development in male sterile floret. In the male sterile plants, the tapetum intrusion after meiosis was less remarkable, the microspores started to abort at vacuolate stage, the connective tissue collapsed, and endothecium failed to expand normally and did not undergo cell wall lignification, which prevented anther opening since the septum and stomium were not disrupted. Crosses undertaken in order to introduce the CMS 524 into two different nuclear backgrounds gave rise to morphologically diversified progenies due to different nuclear-mitochondrial interactions. Macroscopic and cytological investigations showed that pollen-donor plants belonging to Jupiter population had potential capacity to restore fertility while the CC line could be considered as a sterility maintainer.

  6. High-density genetic maps for loci involved in nuclear male sterility (NMS1) and sporophytic self-incompatibility (S-locus) in chicory (Cichorium intybus L., Asteraceae).

    Science.gov (United States)

    Gonthier, Lucy; Blassiau, Christelle; Mörchen, Monika; Cadalen, Thierry; Poiret, Matthieu; Hendriks, Theo; Quillet, Marie-Christine

    2013-08-01

    High-density genetic maps were constructed for loci involved in nuclear male sterility (NMS1-locus) and sporophytic self-incompatibility (S-locus) in chicory (Cichorium intybus L.). The mapping population consisted of 389 F1' individuals derived from a cross between two plants, K28 (male-sterile) and K59 (pollen-fertile), both heterozygous at the S-locus. This F1' mapping population segregated for both male sterility (MS) and strong self-incompatibility (SI) phenotypes. Phenotyping F1' individuals for MS allowed us to map the NMS1-locus to linkage group (LG) 5, while controlled diallel and factorial crosses to identify compatible/incompatible phenotypes mapped the S-locus to LG2. To increase the density of markers around these loci, bulked segregant analysis was used. Bulks and parental plants K28 and K59 were screened using amplified fragment length polymorphism (AFLP) analysis, with a complete set of 256 primer combinations of EcoRI-ANN and MseI-CNN. A total of 31,000 fragments were generated, of which 2,350 showed polymorphism between K59 and K28. Thirteen AFLP markers were identified close to the NMS1-locus and six in the vicinity of the S-locus. From these AFLP markers, eight were transformed into sequence-characterized amplified region (SCAR) markers and of these five showed co-dominant polymorphism. The chromosomal regions containing the NMS1-locus and the S-locus were each confined to a region of 0.8 cM. In addition, we mapped genes encoding proteins similar to S-receptor kinase, the female determinant of sporophytic SI in the Brasicaceae, and also markers in the vicinity of the putative S-locus of sunflower, but none of these genes or markers mapped close to the chicory S-locus. PMID:23689744

  7. Transannular cyclisation reactions of the germacrane system mediated by enzymes from Cichorium intybus.

    NARCIS (Netherlands)

    Piet, D.P.

    1996-01-01

    Chicory ( Cichorium intybus L.), one of the many species of the Compositae family, has been cultivated for the production of the leaves since 300 BC as a food supplement and since the 16th century as a substitute for coffee. The sprouts of the chicory are appreciated for their bitter taste. This bit

  8. Construction and characterization of two BAC libraries representing a deep-coverage of the genome of chicory (Cichorium intybus L., Asteraceae

    Directory of Open Access Journals (Sweden)

    Gonthier Lucy

    2010-08-01

    Full Text Available Abstract Background The Asteraceae represents an important plant family with respect to the numbers of species present in the wild and used by man. Nonetheless, genomic resources for Asteraceae species are relatively underdeveloped, hampering within species genetic studies as well as comparative genomics studies at the family level. So far, six BAC libraries have been described for the main crops of the family, i.e. lettuce and sunflower. Here we present the characterization of BAC libraries of chicory (Cichorium intybus L. constructed from two genotypes differing in traits related to sexual and vegetative reproduction. Resolving the molecular mechanisms underlying traits controlling the reproductive system of chicory is a key determinant for hybrid development, and more generally will provide new insights into these traits, which are poorly investigated so far at the molecular level in Asteraceae. Findings Two bacterial artificial chromosome (BAC libraries, CinS2S2 and CinS1S4, were constructed from HindIII-digested high molecular weight DNA of the contrasting genotypes C15 and C30.01, respectively. C15 was hermaphrodite, non-embryogenic, and S2S2 for the S-locus implicated in self-incompatibility, whereas C30.01 was male sterile, embryogenic, and S1S4. The CinS2S2 and CinS1S4 libraries contain 89,088 and 81,408 clones. Mean insert sizes of the CinS2S2 and CinS1S4 clones are 90 and 120 kb, respectively, and provide together a coverage of 12.3 haploid genome equivalents. Contamination with mitochondrial and chloroplast DNA sequences was evaluated with four mitochondrial and four chloroplast specific probes, and was estimated to be 0.024% and 1.00% for the CinS2S2 library, and 0.028% and 2.35% for the CinS1S4 library. Using two single copy genes putatively implicated in somatic embryogenesis, screening of both libraries resulted in detection of 12 and 13 positive clones for each gene, in accordance with expected numbers. Conclusions This

  9. Anthelmintic activity of chicory (Cichorium intybus): in vitro effects on swine nematodes and relationship to sesquiterpene lactone composition

    DEFF Research Database (Denmark)

    Williams, Andrew; Pena-Espinoza, Miguel Angel; Boas, Ulrik;

    2016-01-01

    Chicory is a perennial crop that has been investigated as a forage source for outdoor-reared ruminants and pigs, and has been reported to have anthelmintic properties. Here, we investigated in vitro anthelmintic effects of forage chicory-extracts against the highly prevalent swine parasites Ascaris...... suum and Oesophagostomum dentatum. Methanol extracts were prepared and purified from two different cultivars of chicory (Spadona and Puna II). Marked differences were observed between the anthelmintic activity of extracts from the two cultivars. Spadona extracts had potent activity against A. suum...... anthelmintic activity of forage chicory towards swine nematodes. Our results indicate a significant anthelmintic effect, which may possibly be related to SL composition....

  10. Anti-inflammatory activity of roots of Cichorium intybus due to its inhibitory effect on various cytokines and antioxidant activity

    OpenAIRE

    Waseem Rizvi; Mohd Fayazuddin; Syed Shariq; Ompal Singh; Shagufta Moin; Kafil Akhtar; Anil Kumar

    2014-01-01

    Background: Cichorium intybus L. commonly known as chicory is one of the important medicinal plants commonly used in Ayurvedic system of medicine. It is commonly used for the treatment of diseases involving a khapa and pitta doshas. Traditionally, C. intybus is used for the treatment of inflammatory conditions, but there are only few in vitro studies reporting the anti-inflammatory activity of roots of chicory. Objective: Evaluation of anti-inflammatory activity of roots of chicory and me...

  11. In vivo effect of dried chicory root (Cichorium intybus L.) on xenobiotica metabolising cytochrome P450 enzymes in porcine liver

    OpenAIRE

    Rasmussen, Martin Krøyer; Zamaratskaia, Galia; Ekstrand, Bo

    2011-01-01

    Cytochrome P450 (CYP) enzymes are widely studied for their involvement in metabolism of drugs and endogenous compounds. In porcine liver, CYP1A2,2Aand 2E1 are important for the metabolism of skatole.Feeding chicory roots to pigs is known to decrease the skatole concentration in plasma and fat. In the present study we investigated the effect of chicory on CYP mRNA and protein expression, as well as their activity. Male pigs were feed dried chicory root for 16 days before liver samples were col...

  12. Inclusion of Chicory (Cichorium intybus L.) in Pigs' Diets Affects the Intestinal Microenvironment and the Gut Microbiota

    OpenAIRE

    Liu, Haoyu; Ivarsson, Emma; Dicksved, Johan; Lundh, Torbjörn; Lindberg, Jan Erik

    2012-01-01

    The content and composition of prebiotic plant fiber in the diet is important in promoting gut-related health. This study investigated the effects of the dietary inclusion of chicory forage and roots on the intestinal microenvironment of pigs. Thirty-seven-week-old pigs were fed 1 of 5 diets for 18 days, including a cereal-based control diet and 4 diets with the inclusion of 80 and 160 g kg−1 of body weight chicory forage (CF80 and CF160), 80 g kg−1 chicory root (CR80), and a mix of 80 g kg−1...

  13. In Silico Analysis and Experimental Validation of Active Compounds from Cichorium intybus L. Ameliorating Liver Injury.

    Science.gov (United States)

    Li, Guo-Yu; Zheng, Ya-Xin; Sun, Fu-Zhou; Huang, Jian; Lou, Meng-Meng; Gu, Jing-Kai; Wang, Jin-Hui

    2015-01-01

    This study aimed at investigating the possible mechanisms of hepatic protective activity of Cichorium intybus L. (chicory) in acute liver injury. Pathological observation, reactive oxygen species (ROS) detection and measurements of biochemical indexes on mouse models proved hepatic protective effect of Cichorium intybus L. Identification of active compounds in Cichorium intybus L. was executed through several methods including ultra performance liquid chromatography/time of flight mass spectrometry (UPLC-TOF-MS). Similarity ensemble approach (SEA) docking, molecular modeling, molecular docking, and molecular dynamics (MD) simulation were applied in this study to explore possible mechanisms of the hepato-protective potential of Cichorium intybus L. We then analyzed the chemical composition of Cichorium intybus L., and found their key targets. Furthermore, in vitro cytological examination and western blot were used for validating the efficacy of the selected compounds. In silico analysis and western blot together demonstrated that selected compound 10 in Cichorium intybus L. targeted Akt-1 in hepatocytes. Besides, compound 13 targeted both caspase-1 and Akt-1. These small compounds may ameliorate liver injury by acting on their targets, which are related to apoptosis or autophagy. The conclusions above may shed light on the complex molecular mechanisms of Cichorium intybus L. acting on hepatocytes and ameliorating liver injury. PMID:26389883

  14. In Silico Analysis and Experimental Validation of Active Compounds from Cichorium intybus L. Ameliorating Liver Injury

    Directory of Open Access Journals (Sweden)

    Guo-Yu Li

    2015-09-01

    Full Text Available This study aimed at investigating the possible mechanisms of hepatic protective activity of Cichorium intybus L. (chicory in acute liver injury. Pathological observation, reactive oxygen species (ROS detection and measurements of biochemical indexes on mouse models proved hepatic protective effect of Cichorium intybus L. Identification of active compounds in Cichorium intybus L. was executed through several methods including ultra performance liquid chromatography/time of flight mass spectrometry (UPLC-TOF-MS. Similarity ensemble approach (SEA docking, molecular modeling, molecular docking, and molecular dynamics (MD simulation were applied in this study to explore possible mechanisms of the hepato-protective potential of Cichorium intybus L. We then analyzed the chemical composition of Cichorium intybus L., and found their key targets. Furthermore, in vitro cytological examination and western blot were used for validating the efficacy of the selected compounds. In silico analysis and western blot together demonstrated that selected compound 10 in Cichorium intybus L. targeted Akt-1 in hepatocytes. Besides, compound 13 targeted both caspase-1 and Akt-1. These small compounds may ameliorate liver injury by acting on their targets, which are related to apoptosis or autophagy. The conclusions above may shed light on the complex molecular mechanisms of Cichorium intybus L. acting on hepatocytes and ameliorating liver injury.

  15. Influence of chicory roots /Cichorium intybus L) on boar taint in entire male pigs and female pigs

    OpenAIRE

    Hansen, L L; Jensen, M.T.; Mejer, H.; Roepstorff, A.; Thamsborg, S.M.; Byrne, D. V.; Karlsson, A H; Hansen-Møller, J.; Tuomola, M.

    2005-01-01

    It is known that pure inulin a fructooligosaccharide extracted from chicory roots can: – reduce boar taint (skatole in backfat and blood) – reduce parasite infection levels when added to specially composed experimental diets • However, the entire chicory roots may, in comparison to inulin: – reduce boar taint more effectively – improve the taste of cooked meat from both male and female pigs – be more effective against parasites when added to normal diet types – contain se...

  16. Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR

    OpenAIRE

    Van Bockstaele Erik; Maroufi Asad; De Loose Marc

    2010-01-01

    Abstract Background Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) is a sensitive technique for quantifying gene expression levels. One or more appropriate reference genes must be selected to accurately compare mRNA transcripts across different samples and tissues. Thus far, only actin-2 has been used as a reference gene for qRT-PCR in chicory, and a full comparison of several candidate reference genes in chicory has not yet been reported. Results Seven candi...

  17. Validation of reference genes for gene expression analysis in chicory (Cichorium intybus using quantitative real-time PCR

    Directory of Open Access Journals (Sweden)

    Van Bockstaele Erik

    2010-02-01

    Full Text Available Abstract Background Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR is a sensitive technique for quantifying gene expression levels. One or more appropriate reference genes must be selected to accurately compare mRNA transcripts across different samples and tissues. Thus far, only actin-2 has been used as a reference gene for qRT-PCR in chicory, and a full comparison of several candidate reference genes in chicory has not yet been reported. Results Seven candidate reference genes, including nicotinamide adenine dinucleotide dehydrogenase (NADHD, actin (ACT, β-tubulin (TUB, glyceraldehyde-3-phosphate-dehydrogenase (GADPH, histone H3 (H3, elongation factor 1-alpha (EF and 18S rRNA (rRNA were selected to study the expression stability for normalisation of gene expression in chicory. Primer specificity and amplification efficiency were verified for each gene. The expression stability of these genes was analysed across chicory root and leaf tissues using geNorm, NormFinder and BestKeeper software. ACT, EF, and rRNA were the most stable genes as identified by the three different analysis methods. In addition, the use of ACT, EF and GAPDH as reference genes was illustrated by analysing 1-FEHII (FEHII expression in chicory root and leaf tissues. These analyses revealed the biological variation in FEHII transcript expression among the tissues studied, and between individual plants. Conclusions geNorm, NormFinder, and BestKeeper analyses indicated that ACT, EF and rRNA had the highest expression stability across leaf and root tissues, while GAPDH and NADHD showed relatively low expression stability. The results of this study emphasise the importance of validating reference genes for qRT-PCR analysis in chicory. The use of the most stable reference genes such as ACT and EF allows accurate normalisation of gene expression in chicory leaf and root tissues.

  18. Anthelmintic activity of chicory (Cichorium intybus): in vitro effects on swine nematodes and relationship to sesquiterpene lactone composition.

    Science.gov (United States)

    Williams, Andrew R; Peña-Espinoza, Miguel A; Boas, Ulrik; Simonsen, Henrik T; Enemark, Heidi L; Thamsborg, Stig M

    2016-05-01

    Chicory is a perennial crop that has been investigated as a forage source for outdoor-reared ruminants and pigs, and has been reported to have anthelmintic properties. Here, we investigated in vitro anthelmintic effects of forage chicory-extracts against the highly prevalent swine parasites Ascaris suum and Oesophagostomum dentatum. Methanol extracts were prepared and purified from two different cultivars of chicory (Spadona and Puna II). Marked differences were observed between the anthelmintic activity of extracts from the two cultivars. Spadona extracts had potent activity against A. suum third (L3) and fourth (L4) - stage larvae, as well as O. dentatum L4 and adults, whereas Puna II extracts had less activity against A. suum and no activity towards O. dentatum L4. Transmission-electron microscopy of A. suum L4 exposed to Spadona extracts revealed only subtle changes, perhaps indicative of a specific anthelmintic effect rather than generalized toxicity. Ultra-high liquid chromatography-mass spectrometry analysis revealed that the purified extracts were rich in sesquiterpene lactones (SL), and that the SL profile differed significantly between cultivars. This is the first report of anthelmintic activity of forage chicory towards swine nematodes. Our results indicate a significant anthelmintic effect, which may possibly be related to SL composition. PMID:26935644

  19. Construction of 12 EST libraries and characterization of a 12,226 EST dataset for chicory (Cichorium intybus root, leaves and nodules in the context of carbohydrate metabolism investigation

    Directory of Open Access Journals (Sweden)

    Boutry Marc

    2009-01-01

    Full Text Available Abstract Background The industrial chicory, Cichorium intybus, is a member of the Asteraceae family that accumulates fructan of the inulin type in its root. Inulin is a low calories sweetener, a texture agent and a health promoting ingredient due to its prebiotic properties. Average inulin chain length is a critical parameter that is genotype and temperature dependent. In the context of the study of carbohydrate metabolism and to get insight into the transcriptome of chicory root and to visualize temporal changes of gene expression during the growing season, we obtained and characterized 10 cDNA libraries from chicory roots regularly sampled in field during a growing season. A leaf and a nodule libraries were also obtained for comparison. Results Approximately 1,000 Expressed Sequence Tags (EST were obtained from each of twelve cDNA libraries resulting in a 12,226 EST dataset. Clustering of these ESTs returned 1,922 contigs and 4,869 singlets for a total of 6,791 putative unigenes. All ESTs were compared to public sequence databases and functionally classified. Data were specifically searched for sequences related to carbohydrate metabolism. Season wide evolution of functional classes was evaluated by comparing libraries at the level of functional categories and unigenes distribution. Conclusion This chicory EST dataset provides a season wide outlook of the genes expressed in the root and to a minor extent in leaves and nodules. The dataset contains more than 200 sequences related to carbohydrate metabolism and 3,500 new ESTs when compared to other recently released chicory EST datasets, probably because of the season wide coverage of the root samples. We believe that these sequences will contribute to accelerate research and breeding of the industrial chicory as well as of closely related species.

  20. Endive, Chicory, and their wild relatives. A systematic and phylogenetic study of Cichorium (Asteraceae)

    OpenAIRE

    Kiers, Annemieke M.

    2000-01-01

    This thesis describes a systematic and phylogenetic analysis of the genus Cichorium. Cichorium is a small genus within the Asteraceae family (tribe Lactuceae) and is well known because of its two widely cultivated species: C. endivia (endive) and C. intybus (chicory). The genus occurs in the Old World and has been introduced in temperate and semi-arid regions elsewhere. An important diagnostic character at the genus and species level is the unique structure of the pappus, which, at the same t...

  1. Identification of phenolic constituents in red chicory salads (Cichorium intybus) by high-performance liquid chromatography with diode array detection and electrospray ionisation tandem mass spectrometry.

    Science.gov (United States)

    Carazzone, Chiara; Mascherpa, Dora; Gazzani, Gabriella; Papetti, Adele

    2013-06-01

    Phenolic acids and flavonoids extracted from several types of Cichorium intybus var. silvestre salads ("Chioggia", "Treviso", "Treviso tardivo", and "Verona") were characterised by high-performance liquid chromatography-electrospray ionisation/mass spectrometry. Among the 64 compounds detected, several hydroxycinnamic acid derivatives including 8 mono- and dicaffeoylquinic acids, 3 tartaric acid derivatives, 31 flavonol and 2 flavone glycosides, as well as 10 anthocyanins were characterised based on UV spectra and MS(n) fragmentation patterns. Furthermore, several isomers of caffeic acid derivatives were distinguished for the first time by their specific mass spectral data. This is the first study reporting the glycosylation type and position of mono- and diglycosylated flavonoids in red salads. PMID:23411215

  2. Antioxidant Components of Cichorium intybus in Vitro and in Vivo

    Energy Technology Data Exchange (ETDEWEB)

    Woo, Hyun Jung; Kim, Ji Hyang; Kim, Jin Kyu [Korea Atomic research Institute, Deajeon (Korea, Republic of); Yang, Deok Cho [Chungbuk National Univ., Cheongju (Korea, Republic of)

    2005-07-01

    Cichorium intybus is a perennial herb of the Compositae Family, native of Europe, west and central Asia, north of Africa and South America. The plant grows to a height of about 50-150 cm. The flowering time of it is from July to September. The flower is lingulate and blue. The basal leaves and the stems of the C. intybus, when they are very tender, are edible. The root, dry and toasted, can be used as a substitute of coffee. Its tuberous roots store inulin as a reserve carbohydrate, which consists of a chain of fructose molecules with a termonal glucose molecule. It may be classified as a fructoligosaccharide, and can be found in more than 30,000 vegetal products. Amongst these vegetables, the chicory roots are outlined for the production of inulin on an industrial scale due to the root's stability in the production of long chains of GFn and constant growth, even in moderate climates. Its use in products with low calories and reduced fat levels, in Europe, the United States and Canada, is already quite widespread. The present experiment was carried out to indicate that C. intybus contains both prooxidant and antioxidant compounds which can act in vitro and in vivo.

  3. Antioxidant Components of Cichorium intybus in Vitro and in Vivo

    International Nuclear Information System (INIS)

    Cichorium intybus is a perennial herb of the Compositae Family, native of Europe, west and central Asia, north of Africa and South America. The plant grows to a height of about 50-150 cm. The flowering time of it is from July to September. The flower is lingulate and blue. The basal leaves and the stems of the C. intybus, when they are very tender, are edible. The root, dry and toasted, can be used as a substitute of coffee. Its tuberous roots store inulin as a reserve carbohydrate, which consists of a chain of fructose molecules with a termonal glucose molecule. It may be classified as a fructoligosaccharide, and can be found in more than 30,000 vegetal products. Amongst these vegetables, the chicory roots are outlined for the production of inulin on an industrial scale due to the root's stability in the production of long chains of GFn and constant growth, even in moderate climates. Its use in products with low calories and reduced fat levels, in Europe, the United States and Canada, is already quite widespread. The present experiment was carried out to indicate that C. intybus contains both prooxidant and antioxidant compounds which can act in vitro and in vivo

  4. Chloroplast degeneration and its inhibition by kinetin in detached leaves of Cichorium intybus L.

    OpenAIRE

    F. Młodzianowski; L. Młodzanowska

    2015-01-01

    In the chicory (Cichorium intybus L. var. sativum cv. Polanowicka) leaves two types of chloroplasts are present differing by their degree of osmiophility of the thylakoid inside. This type of differentiation of chloroplasts has so far been found only in several plant species. The process of chloroplast degeneration in darkness is described. In osmiophilic chloroplasts at certain stage of degeneration minutely layered giant grana were found. Kinetin markedly inhibited the process of chloroplas...

  5. Evaluation of Changes of Factors Related to Liver Function in Serum of Horse by Administration of Cichorium intybus

    OpenAIRE

    Najafzadeh, H.; A.R. Ghadrdan, M. Jalali and F. Alizadeh

    2011-01-01

    Chicory (Cichorium intybus) is a plant that is cultured in some area of Iran, including Khozestan. All of parts of the chicory especially its leave and root have medicinal properties. It is traditionally used for treatment icterus, renal failure, gout and arthritis in human. Important side effects were not reported from this plant. Excretion substances like uric acid are clinically important in some pathological conditions such as urecemia and icterus in horse. However the pharmacological eff...

  6. Determination of Airborne Lead Contamination in Cichorium intybus L. in an Urban Environment

    OpenAIRE

    YAKUPOĞLU, Dilmaç; GÜRAY, Tülin; SARICA, Deniz Yurtsever; Zeki KAYA

    2008-01-01

    The major source of environmental lead (Pb) is the combustion of leaded gasoline. After emission as exhaust, lead in the air falls to earth and contaminates soil and plants. Seventeen wild chicory (Cichorium intybus L.) plant samples were collected from each of 2 urban sites in Ankara, one is located at the road side of major intercity road (heavy traffic) and the other one is located in relatively less traffic area that is about 1 km away from the first location. Lead content of chicory plan...

  7. Dried chicory root (Cichorium intybus L.) as a natural fructan source in rabbit diet: effects on growth performance, digestion and caecal and carcass traits.

    OpenAIRE

    VOLEK, ZDENEK; Marounek, Milan

    2011-01-01

    Three experimental diets were formulated including (per kg) 100 g oats (control diet), 50 g chicory root (Chicory 5 diet) or 100 g chicory root (Chicory 10 diet) at the expense of oats. The diets differed with respect to starch and fructan contents. In the performance trial, 120 Hyplus rabbits were randomly allocated to three groups (two rabbits per cage) and fed one of the three experimental diets between 31 and 73 days of age. At the end of the trial period, 20 rabbits per treatment were...

  8. Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus) show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi

    OpenAIRE

    Miguel Peña-Espinoza; Ulrik Boas; Andrew R. Williams; Thamsborg, Stig M.; Henrik T. Simonsen; Enemark, Heidi L.

    2015-01-01

    The study investigated direct anthelmintic effects of sesquiterpene lactones (SL)-containing extracts from forage chicory against free-living and parasitic stages of Ostertagia ostertagi. Freeze-dried leaves from chicory cultivars ‘Spadona’ and ‘Puna II’ were extracted using methanol/water. Total SL were further fractionated by solid-phase extraction and resulting extracts were characterised by high-performance liquid chromatography (HPLC). O. ostertagi eggs from faeces of mono-infected calve...

  9. Chloroplast degeneration and its inhibition by kinetin in detached leaves of Cichorium intybus L.

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    F. Młodzianowski

    2015-05-01

    Full Text Available In the chicory (Cichorium intybus L. var. sativum cv. Polanowicka leaves two types of chloroplasts are present differing by their degree of osmiophility of the thylakoid inside. This type of differentiation of chloroplasts has so far been found only in several plant species. The process of chloroplast degeneration in darkness is described. In osmiophilic chloroplasts at certain stage of degeneration minutely layered giant grana were found. Kinetin markedly inhibited the process of chloroplast degeneration, and after prolonged treatment even stimulated the stacking. process of grana thylakoids.

  10. Water stress drastically reduces root growth and inulin yield in Cichorium intybus (var. sativum) independently of photosynthesis

    OpenAIRE

    Vandoorne, Bertrand; Mathieu, Anne-Sophie; Van den Ende, Wim; Vergauwen, Rudy; Périlleux, Claire; Javaux, Mathieu; Lutts, Stanley

    2012-01-01

    Root chicory (Cichorium intybus var. sativum) is a cash crop cultivated for inulin production in Western Europe. This plant could be exposed to severe water stress during the three last months of their six months growing period. The aim of this study was to quantify the effect of a progressive decline in water availability on plant growth, photosynthesis and sugar metabolism and to determine its impact on inulin production. Water stress drastically decreased root fresh and dry weight, leaf nu...

  11. Fecal microbiome of growing pigs fed a cereal based diet including chicory (Cichorium intybus L.) or ribwort (Plantago lanceolata L.) forage.

    Energy Technology Data Exchange (ETDEWEB)

    Dicksved J, Johan; Jansson, Janet K.; Lindberg , Jan E.

    2015-12-18

    BACKGROUND: The purpose of this study was to investigate how inclusion of chicory forage or ribwort forage in a cereal-based diet influenced the fecal microbial community (microbiome) in newly weaned (35 days of age) piglets. The piglets were fed a cereal-based diet without (B) and with inclusion (80 and 160 g/kg air-dry forage) of vegetative shoots of chicory (C) and leaves of ribwort (R) forage in a 35-day growth trial. Fecal samples were collected at the start (D0), 17 (D17) and 35 (D35) days after weaning and profiles of the microbial consortia were generated using terminal restriction fragment length polymorphism (T-RFLP). 454-FLX pyrosequencing of 16S rRNA gene amplicons was used to analyze the microbial composition in a subset of the samples already analyzed with T-RFLP. RESULTS: The microbial clustering pattern was primarily dependent on age of the pigs, but diet effects could also be observed. Lactobacilli and enterobacteria were more abundant at D0, whereas the genera Streptococcus, Treponema, Clostridium, Clostridiaceae1 and Coprococcus were present in higher abundances at D35. Pigs fed ribwort had an increased abundance of sequences classified as Treponema and a reduction in lactobacilli. However, the abundance of Prevotellaceae increased with age in on both the chicory and the ribwort diet. Moreover, there were significant correlations between the abundance of Bacteroides and the digested amount of galactose, uronic acids and total non-starch polysaccharides, and between the abundance of Bacteroidales and the digested amount of xylose. CONCLUSION: This study demonstrated that both chicory and ribwort inclusion in the diet of newly weaned pigs influenced the composition of the fecal microbiota and that digestion of specific dietary components was correlated with species composition of the microbiota. Moreover, this study showed that the gut will be exposed to a dramatic shift in the microbial community structure several weeks after weaning.

  12. Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus) show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi.

    Science.gov (United States)

    Peña-Espinoza, Miguel; Boas, Ulrik; Williams, Andrew R; Thamsborg, Stig M; Simonsen, Henrik T; Enemark, Heidi L

    2015-12-01

    The study investigated direct anthelmintic effects of sesquiterpene lactones (SL)-containing extracts from forage chicory against free-living and parasitic stages of Ostertagia ostertagi. Freeze-dried leaves from chicory cultivars 'Spadona' and 'Puna II' were extracted using methanol/water. Total SL were further fractionated by solid-phase extraction and resulting extracts were characterised by high-performance liquid chromatography (HPLC). O. ostertagi eggs from faeces of mono-infected calves were hatched and L1 were used in a larval feeding inhibition assay (LFIA), while cultured L3 were used in a larval exsheathment inhibition assay (LEIA). Adult worms were immediately recovered after slaughter and used for motility inhibition assays (AMIA). Electron microscopy (EM) was performed on adult O. ostertagi exposed to 1000 μg extract mL(-1) of both chicory cultivars. In all assays, decreasing concentrations of SL-containing extracts in PBS (1% DMSO) were tested in replicates with 1% DMSO in PBS as negative controls. HPLC demonstrated similar concentrations of most SL in both extracts. However, Spadona-extract contained significantly higher concentrations of 11, 13-dihydro-8-deoxylactucin (P = 0.01), while Puna II-extract had increased levels of 11, 13-dihydrolactucin (P  0.05). In the AMIA, both SL-containing extracts induced a dose-dependent effect but Spadona-extract showed greater activity and exerted faster worm paralysis than Puna II-extract with significantly lower EC50 (P inhibit feeding of free-living larvae and exert direct effects against parasitic stages of O. ostertagi. Our results may contribute to the identification of natural anti-parasitic compounds and to interpret the in vivo anthelmintic effects of forage chicory. PMID:27120066

  13. Sesquiterpene lactone content and overall quality of fresh-cut witloof chicory (Cichorium intybus L. var. foliosum Hegi) as affected by different washing procedures.

    Science.gov (United States)

    Wulfkuehler, Sabine; Gras, Claudia; Carle, Reinhold

    2013-08-14

    Fresh-cut chicory was produced by four different processing lines testing cold and warm water (4/45 °C, 120 s) and cold water containing cysteine (0.5 mM) against an unwashed control. Levels of sesquiterpene lactones (SLs), sensory, and color attributes as well as O2 and CO2 levels in the modified atmosphere of the fresh-cut produce stored in consumer-sized bags (4 °C, 10 days) were monitored. All washing procedures applied significantly reduced the SL contents and bitterness in fresh-cut chicory. Warm water washing was most effective, reducing the total SL levels by 60.9-64.5%. Sensory and color attributes were also retained best by warm water washing, suggesting such treatments to be suitable measures to improve the quality and reduce the bitterness of fresh-cut chicory. SL profiles changed substantially in all samples during storage, revealing lactucopicrin to be the major SL directly after processing, whereas 11(S),13-dihydrolactuin prevailed at the end of storage. PMID:23905988

  14. Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi

    Directory of Open Access Journals (Sweden)

    Miguel Peña-Espinoza

    2015-12-01

    Full Text Available The study investigated direct anthelmintic effects of sesquiterpene lactones (SL-containing extracts from forage chicory against free-living and parasitic stages of Ostertagia ostertagi. Freeze-dried leaves from chicory cultivars ‘Spadona’ and ‘Puna II’ were extracted using methanol/water. Total SL were further fractionated by solid-phase extraction and resulting extracts were characterised by high-performance liquid chromatography (HPLC. O. ostertagi eggs from faeces of mono-infected calves were hatched and L1 were used in a larval feeding inhibition assay (LFIA, while cultured L3 were used in a larval exsheathment inhibition assay (LEIA. Adult worms were immediately recovered after slaughter and used for motility inhibition assays (AMIA. Electron microscopy (EM was performed on adult O. ostertagi exposed to 1000 μg extract mL−1 of both chicory cultivars. In all assays, decreasing concentrations of SL-containing extracts in PBS (1% DMSO were tested in replicates with 1% DMSO in PBS as negative controls. HPLC demonstrated similar concentrations of most SL in both extracts. However, Spadona-extract contained significantly higher concentrations of 11, 13-dihydro-8-deoxylactucin (P = 0.01, while Puna II-extract had increased levels of 11, 13-dihydrolactucin (P  0.05. In the AMIA, both SL-containing extracts induced a dose-dependent effect but Spadona-extract showed greater activity and exerted faster worm paralysis than Puna II-extract with significantly lower EC50 (P < 0.0001. No cuticular damage was observed by EM in worms exposed to any of the extracts. We have demonstrated that SL-containing extracts from forage chicory can inhibit feeding of free-living larvae and exert direct effects against parasitic stages of O. ostertagi. Our results may contribute to the identification of natural anti-parasitic compounds and to interpret the in vivo anthelmintic effects of forage chicory.

  15. FLAVONE AND FLAVONE GLYCOSIDE FROM CICHORIUM INTYBUS LINN.

    OpenAIRE

    Gautam Patil et al.

    2012-01-01

    Cichorium intybus Linn (Compositae) contains UV-absorbing metabolites. Studies on methanol extract of Cichorium intybus Linn by means of HPLC-UV, NMR, HPLC-MS resulted in isolation and identification of three previously unknown flavones glycosides: 5, 6, 7, 3′, 4′, 7′-hexahydroxy flavones-7-O-β-D-glucopyranoside (1), 3, 5, 7 – trihydroxy-3′,4′-dimethoxy-flavone-7-O-β-D-galactopyranosyl-(1→4)-β-D-xylopyranosyl-3-O-α-L-rhamnopyranoside (2), 3, 5, 7-trihydroxy-6, 4′-dimethoxy flavones (3) also ...

  16. A cultura da chicória para café (Cichorium intybus L.) na região do Ribatejo

    OpenAIRE

    Gamito, João Lúcio Crujo

    2010-01-01

    Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia This work focuses on the culture of industrial chicory, also named “coffee” chicory (Cichorium intybus L. var. sativum), made in Ribatejo in 2008, especially in what concerns agricultural component. It was aimed mainly to assess the conditions for the development of roots, fertilization needs, fight against weeds, diseases and the evaluation of general behaviour of three cultivars of this crop: hera, orchies, a...

  17. [Near-infrared reflectance spectroscopy analytic model established for the IVDMD of Cichorium intybus L].

    Science.gov (United States)

    Hu, Chao; Bai, Shi-qie; Zhang, Yu; Yan, Jia-jun; You, Ming-hong; Li, Da-xu; Bai, Ling; Zhang Jin

    2014-08-01

    Chicory (Cichorium intybus L.) is a new type of forage grasses of high yield and quality with a great value of popularization and utilization. In vitro dry matter digestibility (IVDMD) is one of the important indicators of the nutritional value of forage evaluation. For the study of establishment of Chicory IVDMD NIRS quantitative analysis model, seventy-two species with different genotypes, different growth stages of 204 chicory samples of aboveground material were collected, and by Fourier transform near-infrared diffuse reflectance spectroscopy, through the use of different regression algorithms, can comparing different spectral ranges and spectral pretreatment methods, eight chicory IVDMD NIRS calibration models were established, and the best calibration model parameters were chosen. Its calibration coefficient of determination (Ri) and external validation coefficient of determination (Rval2) were 0.95317 and 0.90455, calibration standard deviation (RMSEC) and predictive standard deviation (RMSEP) was 1.977 99% and 2.008 82%, and the correlation coefficient (r) between predicted values and chemical values was 0.95108. The results show that using NIRS to determine chicory IVDMD is feasible, and provided a rapid analysis method for the determination IVDMD of chicory. PMID:25508718

  18. Culture conditions for production of 2-1-β-D-Fructan-fructanohydrolase in solid culturing on chicory (Cichorium intybus roots

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    Ashok Pandey

    1998-01-01

    Full Text Available Investigations were carried out to optimize the culture conditions for the production of b-D-fructan-fructanohydrolase by an indigenously isolated bacterial culture of Staphylococcus sp. RRL8. Experiments were carried out in solid culturing using chicory roots (powdered as the source of carbon, which was supplemented, with corn steep liquor and potassium dihydrogen phosphate. A number of process parameters, like period of cultivation, initial moisture content in the substrate and temperature of incubation were optimized. Maximum extra-cellular enzyme was produced when fermentation was carried out at 30ºC for 24 h using chicory roots with 60% initial moisture. Supplementation of the substrate with additional carbon source (except with sucrose resulted decreased enzyme titres, which indicated that the strain was partially depressive. Addition of external nitrogen sources (in addition to corn steep liquor also failed to stimulate enzyme formation; rather exerted harmful impact on bacterial culture of Staphylococcus sp. RRL8.Estudos foram realizados com o objetivo de otimizar as condições de cultura para a produção de b-D-fructan-fructanohydrolase (inulinase com uma cepa selvagem de Estafilococos sp. RRL8. Os experimento foram realizados em meio sólido utilizando raiz de chicória moída como única fonte de carbono e suplementada com licor de milho e KH2P0(4 p. Uma certo número de parâmetros, tais como tempo de cultivo, umidade inicial e temperatura de incubação foram otimizadas. A produção máxima de enzima extra-celular foi obtida quando a fermentação foi realizada à temperatura de 30ºC por um período de 24 horas e um teor umidade de 60%. A suplementação do substrato com uma fonte de carbono adicional (exceto com sacarose resultou no decréscimo da produção de enzima, o que indica que a cepa foi parcialmente depressiva. A adição de uma fonte de nitrogênio externa também não resultou em maior de produção da enzima, ao

  19. FLAVONE AND FLAVONE GLYCOSIDE FROM CICHORIUM INTYBUS LINN.

    Directory of Open Access Journals (Sweden)

    Gautam Patil et al.

    2012-03-01

    Full Text Available Cichorium intybus Linn (Compositae contains UV-absorbing metabolites. Studies on methanol extract of Cichorium intybus Linn by means of HPLC-UV, NMR, HPLC-MS resulted in isolation and identification of three previously unknown flavones glycosides: 5, 6, 7, 3′, 4′, 7′-hexahydroxy flavones-7-O-β-D-glucopyranoside (1, 3, 5, 7 – trihydroxy-3′,4′-dimethoxy-flavone-7-O-β-D-galactopyranosyl-(1→4-β-D-xylopyranosyl-3-O-α-L-rhamnopyranoside (2, 3, 5, 7-trihydroxy-6, 4′-dimethoxy flavones (3 also isolated were three known flavones, luteolin (4, ladanetin (5 and spicoside (6.

  20. EMS induced karyomorphological variations in Cichorium intybus L.

    OpenAIRE

    Iram Fatma Jafri *, Ainul Haq Khan, Mohd Gulfishan And M.Y.K. Ansari

    2011-01-01

    Cytogenetical investigations were carried out in Cichorium intybus plants derived from EMS treated seeds at 0.1, 0.2, 0.3, 0.4 and0.5% concentrations. The plants in the treated populations showed varying degrees of meiotic irregularities almost at allmutagenic concentrations. Variations in some parameters such as seed germination, pollen fertility, days to flowering, days tomaturity, number of leaves per plant, leaf length, plant height, and chromosome behavior have been studied in M1 generat...

  1. Mycorrhizal fungi modulate phytochemical production and antioxidant activity of Cichorium intybus L. (Asteraceae) under metal toxicity.

    Science.gov (United States)

    Rozpądek, P; Wężowicz, K; Stojakowska, A; Malarz, J; Surówka, E; Sobczyk, Ł; Anielska, T; Ważny, R; Miszalski, Z; Turnau, K

    2014-10-01

    Cichorium intybus (common chicory), a perennial plant, common in anthropogenic sites, has been the object of a multitude of studies in recent years due to its high content of antioxidants utilized in pharmacy and food industry. Here, the role of arbuscular mycorrhizal fungi (AMF) in the biosynthesis of plant secondary metabolites and the activity of enzymatic antioxidants under toxic metal stress was studied. Plants inoculated with Rhizophagus irregularis and non-inoculated were grown on non-polluted and toxic metal enriched substrata. The results presented here indicate that AMF improves chicory fitness. Fresh and dry weight was found to be severely affected by the fungi and heavy metals. The concentration of hydroxycinnamates was increased in the shoots of mycorrhizal plants cultivated on non-polluted substrata, but no differences were found in plants cultivated on metal enriched substrata. The activity of SOD and H2O2 removing enzymes CAT and POX was elevated in the shoots of mycorrhizal plants regardless of the cultivation environment. Photochemical efficiency of inoculated chicory was significantly improved. Our results indicate that R. irregularis inoculation had a beneficial role in sustaining the plants ability to cope with the deleterious effects of metal toxicity. PMID:25048909

  2. Antimicrobial and antioxidant activities of Cichorium intybus root extract using orthogonal matrix design.

    Science.gov (United States)

    Liu, Haitao; Wang, Quanzhen; Liu, Yuyan; Chen, Guo; Cui, Jian

    2013-02-01

    Solvent, impregnation time, sonication repetitions, and ultrasonic power were important factors in the process of ultrasound-assisted extraction from chicory (Cichorium intybus) root, while there were no studies about optimizing these 4 factors for extract yield, total phenolic content (TPC), antioxidant, antibacterial, and antifungal activity of the extracts using orthogonal matrix design. The present research demonstrated that the solvent composition played a significant role in the improving extract yield, TPC, antioxidant, and antibacterial activities. The other 3 factors had inequable effect on different purposes, ultrasonic power could improve TPC and antioxidant activity, but long time of extraction lowered antioxidant activity. The TPC increased from 22.34 to 27.87 mg GAE (gallic acid equivalents)/100 g (dry extracts) with increasing solvent polarity. The half inhibition concentration (IC(50,) μg/mL) of the radical scavenging activity of the chicory extracts ranged from 281.00 to 983.33 μg/mL. The content of caffeoylquinic acids of root extract, which was extracted by the optimal combination was 0.104%. Several extracts displayed antibacterial activities against Escherichia coli, Staphylococcus aureus, Bacillus thuringiensis, Bacillus subtilis, and Salmonella typhi, while Penicillium sp. and Aspergillus sp. resisted against all the extracts. Combination of 70% ethanol v/v, 24-h impregnation time, 3 sonication rounds, and 300-W ultrasonic input power was found to be the optimal combination for the chicory extract yield, TPC, antioxidant activity, and antibacterial activity. PMID:23387896

  3. EMS induced karyomorphological variations in Cichorium intybus L.

    Directory of Open Access Journals (Sweden)

    Iram Fatma Jafri *, Ainul Haq Khan, Mohd Gulfishan And M.Y.K. Ansari

    2011-12-01

    Full Text Available Cytogenetical investigations were carried out in Cichorium intybus plants derived from EMS treated seeds at 0.1, 0.2, 0.3, 0.4 and0.5% concentrations. The plants in the treated populations showed varying degrees of meiotic irregularities almost at allmutagenic concentrations. Variations in some parameters such as seed germination, pollen fertility, days to flowering, days tomaturity, number of leaves per plant, leaf length, plant height, and chromosome behavior have been studied in M1 generation. Apositive correlation between increasing concentrations of mutagen and chromosomal aberration has been observed

  4. PHARMACOGNOSTICAL PROFILES ON CICHORIUM INTYBUS LINN., LEAVES

    Directory of Open Access Journals (Sweden)

    Guguloth Sarvankumar

    2011-11-01

    Full Text Available India has one the oldest, richest and most diverse cultural traditions associated with the use of medicinal plants. The remarkable fact is that is it still living tradition. The members of resistant of microbial pathogens are growing since penicillin resistant and multi resistant microbes cause a major problem. Now compounds inhibiting microorganism, such as benzoin and emotion have been isolated from plants may inhibit bacteria by a different mechanism than the presently used antibiotics and may have clinical value in treatment of resistant microbial strain. The present study shows the Antibacterial & antifungal activity ethanolic extract of leaf of C. intybus Linn was carried out by pour plate technique antibacterial activity of the extract was measured in terms of zone of inhibition. From antimicrobial study, the leaf has very good sensitivity against both gram+ve and gram-ve organisms and fungi. The highest % of zone of inhibition indicates that the leaf can be very well useful in candidiaisi.

  5. Effect of Cichorium intybus L. on fatty liver induced by oxytetracycline in albino rats.

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    Eman G.E. Helal* , Samia M. Abd El-Wahab* , Atef M.Moussa Sharaf**

    2011-10-01

    Full Text Available Background: Fatty liver is now one of the most common diseases in Egypt. People prefer to use the medicinal plants instead of using chemical compounds because they are cheap and have few side effects compared to chemical compounds.The current investigation was carried out to examine the possible potential therapeutic and protective effects of Cichorium intybus (chicory against oxytetracyclin-induced fatty liver in an attempt to understand its mechanism of action, which may pave the way for possible therapeutic applications. Material and Methods: Albino rats were divided into two major groups, 15 rats for each. The first group was divided into three sub-groups: a control, b fatty liver group; that was injected intraperitonealy with oxytetracycline (120mg/kg for three consecutive days resulting in steatosis and c chicory treated group; which was treated with chicory water extract (70 mg/kg for 30 days after fatty liver induction . All animals were scarified after 33 days of the beginning of the experiment. The second group was divided into three subgroups: a control, b fatty liver group; that was injected intraperitonealy with oxytetracycline (120mg/kg for three consecutive days and c drug protection group; which received chicory for 15 days before induction of fatty liver, then sacrificed after induction of fatty liver (3 days. Blood samples were collected for biochemical analysis. Liver specimens were obtained and fixed in 10% formalin for histological study. Results: Fatty liver groups showed high significant increase in serum glucose, cholesterol, triglycerides, LDL cholesterol, ALAT, ASAT, GGT, LDH, urea, creatinine and A/G ratio while total protein, albumin, globulin and HDL cholesterol were significantly decreased compared to control group. These biochemical changes were accompanied with histopathological alterations in fatty liver tissue. The treatment with chicory ameliorated most of the evaluated biochemical parameters and improved the

  6. The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model.

    Science.gov (United States)

    Azzini, Elena; Maiani, Giuseppe; Garaguso, Ivana; Polito, Angela; Foddai, Maria S; Venneria, Eugenia; Durazzo, Alessandra; Intorre, Federica; Palomba, Lara; Rauseo, Maria L; Lombardi-Boccia, Ginevra; Nobili, Fabio

    2016-01-01

    Phytochemicals can exert their bioactivity without reaching the systemic circulation; scarcely absorbed antioxidants might reach the large bowel contributing to protection from oxidative damage-induced gastrointestinal diseases. In the present work, we aimed to study the relationship between potential activity of polyphenol-rich extracts from Cichorium intybus L. and changes in morphological characteristics on Caco-2 cells. Phytochemicals content (carotenoids and flavonoids) and total antioxidant activity of Red Chicory of Treviso and Variegated Chicory of Castelfranco were evaluated. The bioactivity of polyphenol-rich extracts from chicories was studied in in vitro Caco-2 cell monolayers model. Morphological characteristics changes to test the antioxidant and/or prooxidant effect were verified by histological analysis and observed by Electronic Scansion Microscopy (SEM). On Caco-2 cell model, the polyphenols fractions from chicories have indicated a moderate antioxidant behavior until 17 μM concentration, while 70 μM and 34 μM exert cytotoxic effects for Treviso's and Castelfranco's Chicory, respectively, highlighted by TEER decreasing, increased permeability, and alteration of epithelium. Our findings support the beneficial effects of these products in counteracting the oxidative stress and cellular damage, induced in vitro on Caco-2 cell model, through interaction with the mucopolysaccharide complexes in the glycocalyx, maintaining in vivo a healthy and effective intestinal barrier. PMID:26843906

  7. The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model

    Directory of Open Access Journals (Sweden)

    Elena Azzini

    2016-01-01

    Full Text Available Phytochemicals can exert their bioactivity without reaching the systemic circulation; scarcely absorbed antioxidants might reach the large bowel contributing to protection from oxidative damage-induced gastrointestinal diseases. In the present work, we aimed to study the relationship between potential activity of polyphenol-rich extracts from Cichorium intybus L. and changes in morphological characteristics on Caco-2 cells. Phytochemicals content (carotenoids and flavonoids and total antioxidant activity of Red Chicory of Treviso and Variegated Chicory of Castelfranco were evaluated. The bioactivity of polyphenol-rich extracts from chicories was studied in in vitro Caco-2 cell monolayers model. Morphological characteristics changes to test the antioxidant and/or prooxidant effect were verified by histological analysis and observed by Electronic Scansion Microscopy (SEM. On Caco-2 cell model, the polyphenols fractions from chicories have indicated a moderate antioxidant behavior until 17 μM concentration, while 70 μM and 34 μM exert cytotoxic effects for Treviso’s and Castelfranco’s Chicory, respectively, highlighted by TEER decreasing, increased permeability, and alteration of epithelium. Our findings support the beneficial effects of these products in counteracting the oxidative stress and cellular damage, induced in vitro on Caco-2 cell model, through interaction with the mucopolysaccharide complexes in the glycocalyx, maintaining in vivo a healthy and effective intestinal barrier.

  8. Evaluation of Changes of Factors Related to Liver Function in Serum of Horse by Administration of Cichorium intybus

    Directory of Open Access Journals (Sweden)

    H. Najafzadeh

    2011-02-01

    Full Text Available Chicory (Cichorium intybus is a plant that is cultured in some area of Iran, including Khozestan. All of parts of the chicory especially its leave and root have medicinal properties. It is traditionally used for treatment icterus, renal failure, gout and arthritis in human. Important side effects were not reported from this plant. Excretion substances like uric acid are clinically important in some pathological conditions such as urecemia and icterus in horse. However the pharmacological effect of chicory was not evaluated in horses. The present study was conducted for evaluation effect of leave of chicory on changes of factors related to liver function in serum of horse. In this study, 8 Arabian horses were selected. They had 10-20 years and were clinically in normal conditions. The horses were fed routine diet. The dried leave of chicory was daily added to food of horses at 0.5 g/kg for 15 days. The blood of horses was daily collected before, during and 6 days after chicory administration. The serum was isolated and uric acid, Alanine Transferase (ALT, Aspartate Transferase (AST, Alkaline Phosphatase (ALP, Lactate Dehydrogenase (LDH, conjugated and total billirubin, total protein and albumin concentrations were measured. The mean of these factors were statistically compared. Chicory consumption did not statistically change concentration of above factors. Thus, chicory dose not affect concentration of ALT, AST, ALP, LDH, conjugated and total billirubin, total protein and albumin and uric acid in serum of horse in normal condition; but it may be benefit in pathological conditions.

  9. Identification of organic acids in Cichorium intybus inhibiting virulence-related properties of oral pathogenic bacteria.

    Science.gov (United States)

    Papetti, Adele; Mascherpa, Dora; Carazzone, Chiara; Stauder, Monica; Spratt, David A; Wilson, Michael; Pratten, Jonathan; Ciric, Lena; Lingström, Peter; Zaura, Egija; Weiss, Ervin; Ofek, Itzak; Signoretto, Caterina; Pruzzo, Carla; Gazzani, Gabriella

    2013-06-01

    The low molecular mass (LMM) extract of Cichorium intybus var. silvestre (red chicory) has been shown to inhibit virulence-linked properties of oral pathogens including Streptococcus mutans, Actinomyces naeslundii and Prevotella intermedia. In the present study HPLC-DAD-ESI/MS(2) was used to investigate the compounds contained in this extract for their anti-virulence activity. The extract contained a number of components, including oxalic, succinic, shikimic and quinic acids, which interfere with the growth and virulence traits (i.e., biofilm formation, adherence to epithelial cells and hydroxyapatite) of oral pathogens involved in gingivitis and tooth decay. Succinic and quinic acid seem to be the most potent, mainly by interfering with the ability of oral pathogens to form biofilms (either through inhibition of their development or promotion of their disruption). Our findings suggest that one or more of these compounds may modulate plaque formation in vivo, which is a prerequisite for the development of both caries and gingivitis. PMID:23411301

  10. Cytotoxicity activities of chloroform extract of Cichorium intybus seed against HCT-15 and Vero cell line

    OpenAIRE

    Prashant Y Mali

    2015-01-01

    Background: Cichorium intybus L., (Asteraceae) is well-known as a coffee substitute but is also widely used medicinally to treat various ailments ranging from wounds to diabetes. Other plant parts are also used for liver and cancer disorder. Objective: The objective was to study the cytotoxic potential of chloroform extract of C. intybus seed against HCT-15 and Vero (normal) cell line. Materials and Methods: Fourier transform infrared spectroscopy (FTIR) analysis of the extract was performed....

  11. The effects of cichorium intybus extract on the maturation and activity of dendritic cells

    OpenAIRE

    Karimi, Mohammad Hossein; Ebrahimnezhad, Salimeh; Namayandeh, Mandana; Amirghofran, Zahra

    2014-01-01

    Background Cichorium intybus is a medicinal plant commonly used in traditional medicine for its benefits in immune-madiated disorders. There are several evidences showing that C. intybus can modulate immune responses. In the present study we have investigated the effects of the ethanolic root extract of this plant on the immune system by targeting dendritic cells (DCs). For this purpose, phenotypic and functional maturity of murine DCs after treatment with the extract was analyzed by flow cyt...

  12. ANTIDIABETIC ACTIVITY OF CICHORIUM INTYBUS L. SEEDS ON STZ-INDUCED DIABETIC RATS

    OpenAIRE

    Raad A Kaskoos

    2012-01-01

    Diabetes mellitus is a chronic disorder of metabolism caused by an absolute or relative lack of insulin. WHO recommends evaluation of the antidiabetic potential of more and more plants to find a tenable cure for the disease. The folkloric claim that Cichorium intybus seeds are very effective in diabetes and its manifestations prompted us to evaluate its antidiabetic potential. C. intybus L. (Asteraceae), popularly known as Kasini, was first introduced by Prophet Mohammed 1400 years ago. The p...

  13. 盐胁迫对菊苣幼苗脯氨酸积累及其代谢途径的影响%Effects of NaCl Stress on Proline Accumulation and Metabolic Pathways of Chicory (Cichorium intybus L. ) Seedlings

    Institute of Scientific and Technical Information of China (English)

    王康; 刘艳香; 董洁; 周禾; 董宽虎

    2011-01-01

    Proline plays important roles in regulation of osmotic homeostasis, prevention of damages caused by osmotic stresses, scavenging of reactive oxygen species and protection of cell structures. Many plants accumulate proline dramatically under a variety of stress conditions. The regulation of proline accumulation in Chicory seedlings and the activities of key enzymes involved in proline metabolism in response to salinity were studied here. Fifty-day-old seedlings were treated with NaCl (0, 100, 200, 300 and 400 mM) in this experiment. Results show that Ornithine-oxo-acid transaminase (δ-OAT) activity increases; Pyrroline-5-carboxylate synthetase (P5CS) activity initially increases then deccreases, while ProDH activity decreases with increasing NaCl concentration. Proline contents increase significantly (P<0.05) with increasing saltstress followed by a declining response during the extending salt-stress period. The data demonstrate that glutamate and ornthine pathways are activated by NaCl. The glutamate pathway is predominant under lowconcentration NaCl treatments, and an ornithine pathway is predominant under high-concentration NaCl treatments.%以菊苣(Cichorium intybus L.)为材料,研究不同浓度NaCl处理对菊苣幼苗体内脯氨酸代谢的影响,以期揭示菊苣中脯氨酸代谢的基本规律.结果表明:随着处理浓度的增加,菊苣中鸟氨酸δ-氨基转移酶(δ-OAT)活性呈上升趋势;吡咯咻-5-羧酸合成酶(P5CS)活性呈现先升后降的趋势;脯氨酸脱氢酶(ProDH)活性呈下降趋势;而脯氨酸含量则显著上升(P<0.05),但高浓度长时间处理后略有降低.研究表明,NaCl胁迫下谷氨酸(Glu)途径和鸟氨酸(Orn)途径对菊苣中脯氨酸的积累均有贡献,低浓度胁迫时P5CS起关键作用的Glu途径占主导地位,高浓度胁迫时δ-OAT起关键作用的Orn途径占主导地位.

  14. Produção e renda bruta de rúcula (Eruca sativa Mill. 'Cultivada' e de almeirão (Cichorium intybus L. 'Amarelo' em cultivo solteiro e consorciado Yield and gross income of 'Cultivada' roquette (Eruca sativa Mill and 'Amarelo' chicory (Cichorium intybus L. in mono-cropping and intercropping system

    Directory of Open Access Journals (Sweden)

    Wilmara Corrêa Harder

    2005-08-01

    objective of evaluating yield and gross income in mono-cropping and inter-cropping system. Mono-croppings of 'Cultivada' roquette-R and 'Amarelo' chicory-A, in three rows (A3 and four (A4 rows and R3A4 and R4A3 intercropping systems were studied in randomized block design, with five replications. Harvests were done at 52 days after sowing and at 35 days after the first harvest. For roquette and chicory, respectively, plant heights (26.54 and 24.99 cm, number of leaves per plant (23.4 and 16.40, length (18.62 and 24.68 cm and area of one leaf (66.73 and 177.0 cm² were not influenced by number of rows, neither by intercropping system, but results were greater for the first harvest than the second. Length of chicory leaf was significantly influenced by intercropped system and number of rows interaction, which ranged from 22.74 (R4 up to 26.78 cm (R3A4. Yield of fresh (15.66 and 11.62 t ha-1 and dried (1.33 and 1.00 t ha-1 mass of roquette under four and three rows were significantly different, respectively. Fresh and dried matter of chicory treatments were, respectively, 12.55 and 0.85 t ha-1 for A3, 15.60 and 1.13 t ha-1 for A4, 17.44 and 1.26 t ha-1 under mono-cropping system and 10.72 and 0.72 t ha-1 under intercropping system. LER for the first and the second harvests were 1.87 and 1.76 for R3A4 and 1.56 and 1.58 for R4A3, respectively. R4A3 arrangement could have produced monetary increases of R$ 28,279.80 and R$ 20,065.60 for the second harvest, regarding to roquette and chicory. The best gross income for the second harvest was in R4A3 treatment, with corresponded to 87% of the best gross income of the first harvest for the same treatment, with a shorter 17-day vegetative cycle.

  15. Chicory cultivars differ in sesquiterpene lactone composition

    Science.gov (United States)

    Chicory (Cichorium intybus L.) is a valuable forage species for small ruminants because it is nutritious and contains sesquiterpene lactones (SLs) that have anthelmintic activity. Three SLs, lactucin (lac), 8-deoxylactucin (dol), and lactucopicrin (lpic), occur in chicory leaves. Comprehensive dat...

  16. Characterization of expressed sequence tags obtained by SSH during somatic embryogenesis in Cichorium intybus L

    Directory of Open Access Journals (Sweden)

    Quillet Marie-Christine

    2007-06-01

    Full Text Available Abstract Background Somatic embryogenesis (SE is an asexual propagation pathway requiring a somatic-to-embryonic transition of differentiated somatic cells toward embryogenic cells capable of producing embryos in a process resembling zygotic embryogenesis. In chicory, genetic variability with respect to the formation of somatic embryos was detected between plants from a population of Cichorium intybus L. landrace Koospol. Though all plants from this population were self incompatible, we managed by repeated selfing to obtain a few seeds from one highly embryogenic (E plant, K59. Among the plants grown from these seeds, one plant, C15, was found to be non-embryogenic (NE under our SE-inducing conditions. Being closely related, we decided to exploit the difference in SE capacity between K59 and its descendant C15 to study gene expression during the early stages of SE in chicory. Results Cytological analysis indicated that in K59 leaf explants the first cell divisions leading to SE were observed at day 4 of culture. In contrast, in C15 explants no cell divisions were observed and SE development seemed arrested before cell reactivation. Using mRNAs isolated from leaf explants from both genotypes after 4 days of culture under SE-inducing conditions, an E and a NE cDNA-library were generated by SSH. A total of 3,348 ESTs from both libraries turned out to represent a maximum of 2,077 genes. In silico subtraction analysis sorted only 33 genes as differentially expressed in the E or NE genotype, indicating that SSH had resulted in an effective normalisation. Real-time RT-PCR was used to verify the expression levels of 48 genes represented by ESTs from either library. The results showed preferential expression of genes related to protein synthesis and cell division in the E genotype, and related to defence in the NE genotype. Conclusion In accordance with the cytological observations, mRNA levels in explants from K59 and C15 collected at day 4 of SE

  17. Bacterial leaf spot of radicchio (Cichorium intybus) is caused by Xanthomonas hortorum

    Science.gov (United States)

    Beginning in 2002 a leaf spot disease of radicchio (Cichorium intybus) was observed in Monterey County, California. Lesions were maroon to dark brown in color; in some cases the margins of brown lesions became dark maroon with aging. Each leaf spot was observable from both adaxial and abaxial leaf s...

  18. Regulation of 3β-hydroxysteroid dehydrogenase and sulphotransferase 2A1 gene expression in primary porcine hepatocytes by selected sex-steroids and plant secondary metabolites from chicory (Cichorium intybus L.) and wormwood (Artemisia sp.).

    Science.gov (United States)

    Rasmussen, Martin Krøyer; Ekstrand, Bo

    2014-02-15

    In pigs the endogenously produced compound androstenone is metabolised in the liver in two steps by 3β-hydroxysteroid dehydrogenase (3β-HSD) and sulphotransferase 2A1 (SULT2A1). The present study investigated the effect of selected sex-steroids (0.01-1 μM androstenone, testosterone and estradiol), skatole (1-100 μM) and secondary plant metabolites (1-100 μM) on the expression of 3β-HSD and SULT2A1 mRNA. Additionally the effect of a global methanolic extract of dried chicory root was investigated and compared to previous obtained in vivo effects. Primary hepatocytes were isolated from the livers of piglets (crossbreed: Landrace×Yorkshire and Duroc) and cultured for 24h before treatment for an additionally 24h. RNA was isolated from the hepatocytes and specific gene expression determined by RT-PCR using TaqMan probes. The investigated sex-steroids had no effect on the mRNA expression of 3β-HSD and SULT2A1, while skatole decreased the content of SULT2A1 30% compared to control. Of the investigated secondary plant metabolites artemisinin and scoparone (found in Artemisia sp.) lowered the content of SULT2A1 by 20 and 30% compared to control, respectively. Moreover, we tested three secondary plant metabolites (lactucin, esculetin and esculin) found in chicory root. Lactucin increased the mRNA content of both 3β-HSD and SULT2A1 by 200% compared to control. An extract of chicory root was shown to decrease the expression of both 3β-HSD and SULT2A1. It is concluded that the gene expression of enzymes with importance for androstenone metabolism is regulated by secondary plant metabolites in a complex manner. PMID:24333270

  19. Anthelmintic effects of forage chicory against parasitic nematodes in cattle

    OpenAIRE

    Pena-Espinoza, Miguel Angel; Williams, Andrew; Thamsborg, Stig Milan; Enemark, Heidi

    2014-01-01

    BACKGROUND: Chicory (Cichorium intybus) has potential as a natural anthelmintic in livestock, however evidence of efficacy against cattle nematodes is lacking. Here, we investigated anthelmintic effects of chicory in stabled calves.METHODS: Jersey male calves (2-4 months) were stratified by live weight and allocated randomly to 2 groups: chicory (CHI, n=9) and control (CON, n=6). CHI and CON calves were fed with forage chicory silage (cv. Spadona) and hay ad libitum, resp., for 8 weeks. After...

  20. HIGH FREQUENCY GENETIC TRANSFORMATION OF CICHORIUM INTYBUS L. USING nptII GENE AS A SELECTIVE MARKER.

    Science.gov (United States)

    Matvieieva, N; Shakhovsky, A; Kvasko, O; Kuchuk, N

    2015-01-01

    Cichorium intybus L. is an important vegetable crop used as salad (leaf form) and for the production of coffee substitutes (root form). At the same time these plants can also be used in biotechnologies for synthesis of pharmaceutical proteins. Here we report the possibility of high frequency Agrobacterium rhizogenes- or A. tumefaciens-mediated transformation of C. intybus L. for construction of transgenic "hairy" roots and plants. The used plasmids contained target human interferonifn-α2b gene, Mycobacterium tuberculosis ESAT6:Ag85B antigene esxA::fbpB(ΔTMD) fused gene and human telomerase reverse transcriptase h Tert gene. Using of nptII gene as a selective one was preferable to the bar gene for chicory. In this case the frequency of transgenic plants or "hairy" roots formation was significantly higher. Cultivation of explants on the medium with Basta in concentration 1-2 mg/l have led to plants death or to significant reduction of number of shoots formed. Frequency of "hairy" roots formation varied from 5.9 to 42.3% after A. rhizogenes-mediated transformation. Frequency of regeneration of transgenic plants varied from 10 to 86% after A. tumefaciens-mediated transformation. Both A. rhizogenes- and A. tumefaciens-mediated transformation frequency depended on the type of explants, roots or cotyledons, and vector used. Usage of A. tumefaciens carrying pCB064 plasmid (target esxA:fbpB(ΔTMD) fused gene and nptII selective gene) resulted in the most effective regeneration of transgenic plants with regeneration frequency up to 86%. In the case of chicory A. rhizogenes-mediated transformation the highest regeneration frequency up to 42.3% was demonstrated using p CB161 vector with ifn-α2b target gene and nptII selective gene. PMID:26419064

  1. Efeito das cipselas de Cichorium intybus em ratinhos expostos a CCI4

    OpenAIRE

    Oliveira, Sónia Marlene Rodrigues

    2009-01-01

    Cichorium intybus é amplamente utilizada na medicina tradicional devido aos seus supostos efeitos hepatoprotectores. Este trabalho teve por objectivo estudar os possíveis efeitos protectores do extracto de cipselas (frutos) de C. intybus no fígado, rim, baço, testículo e epidídimo de ratinhos. Realizaram-se análises histopatológicas e bioquímicas. Os efeitos protectores destas cipselas foram estudados por comparação com os controlos negativo e positivo, NaCl 0,9% e CCl4 em duas...

  2. Nutritive value response of forage chicory cultivars to phosphorus fertility

    Science.gov (United States)

    Forage chicory (Cichorium intybus L.) is a productive plant that appears particularly well suited to improving summer yield of pastures in the USA. Poor palatability of some chicory cultivars in locations with low soil phosphorus fertility has been linked to high levels of sesquiterpene lactones, b...

  3. Reestablishing Chicory into Multi-Species Perennial Pastures

    Science.gov (United States)

    Chicory (Cichorium intybus L.) has the potential to provide abundant, high quality forage during periods of drought stress, but poor persistence limits its usefulness in permanent pasture. This experiment compared the ability of three seeding methods to reestablish chicory into a grazed multi-specie...

  4. Yield and persistence response of forage chicory to phosphorus fertility

    Science.gov (United States)

    Forage chicory (Cichorium intybus) is a productive plant that appears particularly well suited to improving summer yield of pastures in the USA. Poor palatability of some chicory cultivars in locations with low soil phosphorus fertility has been linked to high levels of sesquiterpene lactone, a bit...

  5. Cultivar Preference of Lambs Grazing Forage Chicory in Ohio

    Science.gov (United States)

    This project compared grazing preferences of lambs between seven cultivars of forage chicory (Cichorium intybus L.). This on-farm trial was conducted in central Ohio (40.53 degrees N, 82.46 degrees W, 1089 ft above sea level). The chicory was established by using conventional tillage in Bogart Silt...

  6. Response of Forage Chicory Seedlings to Available Soil Phosphorus

    Science.gov (United States)

    Phosphorus fertility may be responsible for observed differences in chemical composition of forage chicory (Cichorium intybus L.) on West Virginia and Pennsylvania soils, but P effects on chicory growth on these soils are unclear. We evaluated the effect of available soil P (ASP) on ‘Puna’, ‘Lacert...

  7. Cotton APX Gene Enhances Stress Tolerance of Cichorium intybus%导入APX基因提高了普那菊苣植株的抗逆性

    Institute of Scientific and Technical Information of China (English)

    张丽君; 程林梅; 杜建中; 王亦学; 郝耀山; 李贵全; 孙毅

    2012-01-01

    采用农杆菌介导的方法,把CaMV35S启动子驱动的来自棉花(Gossypium spp.)的抗坏血酸过氧化物酶(ascorbate peroxidase,APX)基因导人普那菊苣(Cichorium intybus L.cv.Puna).结果表明:经过卡那霉素(Km)筛选和对抗性植株的PCR和Southern杂交分析,证明APX基因成功地整合到普那菊苣基因组中.转APX基因普那菊苣植株对NaCl和甘露醇胁迫表现出一定的抗性,在NaCl浓度为500 mmol·L-1、甘露醇浓度为30g· L-1的条件下,转APX基因不定芽能够正常生根和牛长,转基因植物叶片外植体能够形成愈伤组织和再生植株,而野生型植株不定芽不能正常生根、已生根幼苗不能正常成长,野生型植株叶片不能形成愈伤组织.%The cotton APX gene was transferred into Cichorium intybus L. Cv. Puna by Agrobacterium-mediation. The transgenic chicory plants were first screened by Kanamycin then detected by both PCR and Southern blot approaches. Effects of both sodium chloride and mannitol on chicory calli and seedling growth were studied. Results indicated that transgenic Puna chicory explants could formed calli and grew into normal seedlings on medium containing 500 mmol · L-1 NaCl and 30 g · L-1 mannitol, while non-transgenic explants could not form callus on the same medium.

  8. The temporal development in a hybridizing population of wild and cultivated chicory (Chicorium intybus L.)

    DEFF Research Database (Denmark)

    Sørensen, Bende Storgaard; Kiær, Lars Pødenphant; Bagger Jørgensen, Rikke;

    2007-01-01

    persistence of the hybrids in a natural environment over time. To evaluate this, we studied an experimental hybridizing population of wild and cultivated chicories Wichorium intybus) relative to a previous study on the same population 2 years earlier. We compared the genetic composition, morphology and...

  9. Micro- and megagametogenesis in Cichorium intybus L.: succes sive stages and some symptoms of apospory

    OpenAIRE

    Beatice Longly; B. P. Louant

    2014-01-01

    In Cichorium intybus L., the mode of reproduction could be at the same time sexual and apomicbic. This could explain the contradictions existing in the interpretation of the heritaibidity of the characters in this species. In this particular case, the only way of describing facultative apospory, functional or not, is to proceed to a cytom-orphological study of the megagametogenesis. In the present study, symptoms of apospory have been observed and described.

  10. Preventive Effect of Cichorium Intybus L. Two Extracts on Cerulein-induced Acute Pancreatitis in Mice

    OpenAIRE

    Mohsen Minaiyan; Ali-Reza Ghannadi; Parvin Mahzouni; Ali-Reza Abed

    2012-01-01

    Objectives: Acute pancreatitis is an inflammatory condition of pancreas with sudden onset, high mortality rate and multiple organ failure characteristics. It has been shown that oxygen free radicals have an important role in development of pancreatitis and its complications. Antioxidant, anti-inflammatory, anti-hepatotoxicity and gastroprotective properties of Cichorium intybus L. suggest that this plant may have beneficial effects in the management of acute pancreatitis. Methods: Five in...

  11. Ameliorating effect of chicory (Chichorium intybus L.) fruit extract against 4-tert-octylphenol induced liver injury and oxidative stress in male rats.

    Science.gov (United States)

    Saggu, Shalini; Sakeran, Mohamed I; Zidan, Nahla; Tousson, Ehab; Mohan, Anand; Rehman, Hasibur

    2014-10-01

    The current study was carried out to elucidate the modulating effect of chicory (Cichorium intybus L.) fruit extract (CFR) against 4-tert-OP induced oxidative stress and hepatotoxicity in male rats. Rats were divided into four groups and treated for 8 weeks as follow: group 1: normal control-treated (saline); group 2: chicory fruit extract-treated (100 mg/kg); group 3: 4-tert-OP treated; group 4: 4-tert-OP plus chicory fruit extract. The obtained results revealed that rats which received 4-tert-OP showed a significant increase in liver TBARS and bilirubin, aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and gamma-glutamyl transpeptidase (GGTP) activities. While a significant decrease in the levels of GSH, SOD, catalase recorded. On the other hand, CFR extract succeeded to modulate these observed abnormalities resulting from 4-tert-OP as indicated by the reduction of TBARS and the pronounced improvement of the investigated biochemical and antioxidant parameters. Histopathological evidence, together with observed PCNA and DNA fragmentation, supported the detrimental effect of 4-tert-OP and the ameliorating effect of CFR extract on liver toxicity. So, it could be concluded that chicory has a promising role and it worth to be considered as a natural substance for ameliorating the oxidative stress and hepatic injury induced by 4-tert-OP compound. PMID:25010453

  12. Rheological characterization of chicory root (Cichorium intybus L. inulin solution

    Directory of Open Access Journals (Sweden)

    J. T. C. L. Toneli

    2008-09-01

    Full Text Available Inulin is a polysaccharide frequently used as a sugar or fat replacer in the food industry, which offers the advantage of a functional effect similar to those of dietary fibers. By cooling or freezing an inulin concentrated solution, a more concentrated solution precipitates as a paste-like substance, while the liquid phase forms a diluted solution. In this work, the effect of storage temperature of inulin concentrated solution as well as temperature on the rheological behavior of liquid and precipitated phases obtained from a process of phase separation were evaluated. The precipitated phase of inulin was evaluated under two conditions: pure and formulated with encapsulating agents. It was observed that a reduction in storage temperature resulted in a higher inulin precipitation, which produced higher apparent viscosity values for the precipitated phase. All the samples analyzed had a shear-thinning rheological behavior.

  13. Hepatoprotective Efficacy of Cichorium intybus L. Extract Against Carbon Tetrachloride-induced Liver Damage in Rats.

    Science.gov (United States)

    Elgengaihi, Souad; Mossa, Abdel-Tawab H; Refaie, Amel A; Aboubaker, Doha

    2016-01-01

    The purpose of the study was to assess the phytochemical and hepatoprotective activity of different extracts of dried herb of Cichorium intybus L. against carbon tetrachloride (CCl4) intoxicated male albino rats. The hepatoprotective activity of different extracts at 500 mg/kg body weight was compared with carbon tetrachloride-treated animals. The animals were divided into five groups with six animals in each group. The first group represents control, the second group received carbon tetrachloride, the third received C. intybus, and the fourth group received C. intybus plus carbon tetrachloride. The fifth group received silymarin as hepato-slandered drug. There were significant changes in serum biochemical parameters such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin, albumin, total protein, and γ-glutamyl transferase (GGT) in carbon tetrachloride intoxicated rats, which were restored towards normal values in C. intybus-treated animals. Histopathological examination of liver tissues further substantiated these findings. In conclusion, of this investigation, the results ascertain that the herb extracts of C. intybus possess significant hepatoprotective activity. PMID:26913368

  14. Sesquiterpene lactones and inulin from chicory roots: extraction, identification, enzymatic release and sensory analysis.

    NARCIS (Netherlands)

    Leclercq, E.

    1992-01-01

    Chicory ( Cichorium intybus L.) is one of the many species of the family Compositae. Chicory has been cultivated for the production of leaves or chicons, which have been used as a vegetable since approximately 300 BC, and for its roots, which can be used as a coffee substitute after roasting.

  15. First report of root rot of Chicory caused by Phytophthora cryptogea in Chile

    Science.gov (United States)

    Chicory (Cichorium intybus L. var sativum Bisch.), a relatively new high value crop in Chile, was introduced for commercial production of inulin. Inulins are polysaccharides extracted from chicory tap roots that are used in processed foods due to their beneficial gastrointestinal properties. Approxi...

  16. Yield, Root Growth and Soil Water Content of Drought-Stressed Pasture Mixtures Containing Chicory

    Science.gov (United States)

    Chicory (Cichorium intybus L.) is a deep-rooted forb that has increasingly been investigated for inclusion in pasture mixtures because of its reported drought tolerance and high productivity during summer months. This study examined how adding chicory to pasture mixtures impacted forage yield, root ...

  17. YIELD AND PERSISTENCE OF FORAGE AND ROOT-TYPE CHICORY CULTIVARS

    Science.gov (United States)

    A challenge in managing chicory (Cichorium intybus L.) as a forage is dealing with bolting of flower stalks in spring. Root-type cultivars of chicory with reduced bolting potential are available. An experiment was conducted at Rock Springs, PA to compare forage cultivars and European root-type culti...

  18. Anthelmintic potential of chicory forage is influenced by sesquiterpene lactone composition

    Science.gov (United States)

    The anthelmintic activity of chicory (Cichorium intybus L.) herbage has been attributed to sesquiterpene lactones (SLs). Chicory leaves contain lactucin (LAC), 8-deoxylactucin (DOL), and lactucopicrin (LPIC), but the amounts of these three SLs vary among cultivars. The objective of this study was...

  19. Non-destructive method for internal quality determination of belgian endive (cichorium intybus l.)

    OpenAIRE

    De Baerdemaeker J.; Quenon V.

    2000-01-01

    A method and process were developed to nondestructively measure the length of the floral stalk in Belgian endive Cichorium intybus L. Current X-ray technology proved to be a feasible method. A detection algorithm was developed based on the minimal transmitted intensities along the length. The method is very accurate with an absolute precision of 4.9 mm and allows the study of the influence of storage conditions and time on the Belgian endive internal quality. The growth of the floral stalk is...

  20. Gewöhnliche Wegwarte – Cichorium intybus (Asteraceae) : Blume des Jahres 2009

    OpenAIRE

    Höggemeier, Annette

    2010-01-01

    Die Stiftung Naturschutz Hamburg und Stiftung LOKI SCHMIDT zum Schutze gefährdeter Pflanzen hat zur "Blume des Jahres 2009" die Wegwarte (Cichorium intybus) ausgewählt. Sie bevorzugt einen ähnlichen Lebensraum wie die Nickende Distel, die Blume des Jahres 2008. Die Wegwarte ist aber häufiger zu finden, sie wächst an Weg- und Ackerrändern und auf bäuerlichen Schuttplätzen. Mich hat schon als Kind diese Pflanze fasziniert, deren strahlend blaue Blüten sich morgens öffnen, aber am frühen Nachmit...

  1. Palatability of Forage Chicory Cultivars for Goats

    Science.gov (United States)

    Sesquiterpene lactones (SL) in forage chicory (Cichorium intybus L.) may have anthelmintic activity against gastrointestinal parasites in sheep and goats, but have been implicated in poor palatability of forage. We used three levels of soil P fertilization to influence SL concentrations in three cu...

  2. NUTRITIVE VALUE OF CHICORY AND ENGLISH PLANTAIN

    Science.gov (United States)

    Graziers in the northeast often face forage shortages in midsummer. Chicory (Cichorium intybus L.) and English plantain (Plantago lanceolata L.) have been introduced in the USA as perennial herbs for pastures and have been touted as drought tolerant. We conducted two field-plot experiments at Rock S...

  3. Agrobacterium-mediated transformation of Cichorium intybus L. with interferon-a2b gene

    OpenAIRE

    Kvasko O. Yu.; Gerasymenko I. M.; Shachovsky A. M.; Matvieieva N. A.; Kuchuk N. V.

    2009-01-01

    An efficient method for the plant regeneration and Agrobacterium mediated transformation with interferon-a2b gene has been developed for chicory C. intybus L. cv. Pala rossa. The regeneration with efficiency about 100 % was induced on the MS medium supplemented with 0.5–2.5 mg/l kinetin and 0.5 mg/l NAA. The transformed plantlets were recovered at a frequency 26,9 % on basal medium with 25 mg/l kanamycin. According to PCR-analysis the nptII and ifn-a2b genes were integrated into the genome of...

  4. Agrobacterium-mediated transformation of Cichorium intybus L. with interferon-a2b gene

    Directory of Open Access Journals (Sweden)

    Kvasko O. Yu.

    2009-04-01

    Full Text Available An efficient method for the plant regeneration and Agrobacterium mediated transformation with interferon-a2b gene has been developed for chicory C. intybus L. cv. Pala rossa. The regeneration with efficiency about 100 % was induced on the MS medium supplemented with 0.5–2.5 mg/l kinetin and 0.5 mg/l NAA. The transformed plantlets were recovered at a frequency 26,9 % on basal medium with 25 mg/l kanamycin. According to PCR-analysis the nptII and ifn-a2b genes were integrated into the genome of transformed plants.

  5. ANTIDIABETIC ACTIVITY OF CICHORIUM INTYBUS L. SEEDS ON STZ-INDUCED DIABETIC RATS

    Directory of Open Access Journals (Sweden)

    Raad A. Kaskoos

    2012-05-01

    Full Text Available Diabetes mellitus is a chronic disorder of metabolism caused by an absolute or relative lack of insulin. WHO recommends evaluation of the antidiabetic potential of more and more plants to find a tenable cure for the disease. The folkloric claim that Cichorium intybus seeds are very effective in diabetes and its manifestations prompted us to evaluate its antidiabetic potential. C. intybus L. (Asteraceae, popularly known as Kasini, was first introduced by Prophet Mohammed 1400 years ago. The present study investigated the effect of a single oral administration of aqueous extract of C. intybus seeds on glucose levels in normal, normal hyperglycemic and STZ-induced diabetic rats at 100 mg/kg, 200 mg/kg and 500 mg/kg doses. A moderate dose-dependent antihyperglycaemic activity in STZ-induced diabetic rats was observed. For chronic studies 500 mg/kg was administered for 21 days and its effect on blood-glucose, serum insulin and lipid profile was estimated during the study. The results indicate that the continued administration of C. intybus extract produced a sustained antihyperglycaemic effect in STZ-induced diabetic rats. The insulin levels did not increase significantly. This indicated that the extract did not induce any regeneration of pancreatic b-cells. Thus the possible mechanism may either be increase in glucose utilization in the periphery or decrease in endogenous glucose production in the liver. It also decreased the levels of circulating lipids and lowered the atherogenic index. Thus, our study lends support to the claim for antidiabetic effect of C. intybus by pharmacological experimentation.

  6. Cytotoxicity activities of chloroform extract of Cichorium intybus seed against HCT-15 and Vero cell line

    Directory of Open Access Journals (Sweden)

    Prashant Y Mali

    2015-01-01

    Full Text Available Background: Cichorium intybus L., (Asteraceae is well-known as a coffee substitute but is also widely used medicinally to treat various ailments ranging from wounds to diabetes. Other plant parts are also used for liver and cancer disorder. Objective: The objective was to study the cytotoxic potential of chloroform extract of C. intybus seed against HCT-15 and Vero (normal cell line. Materials and Methods: Fourier transform infrared spectroscopy (FTIR analysis of the extract was performed. 3-(4, 5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide assay was used for evaluation of the cytotoxic potential of chloroform extract of C. intybus seed. Doxorubicin was considered as standard reference drug. The concentrations 1000–0.05 μg/ml was used in the experiment. Result and Discussion: FTIR spectrum showed 1025.363, 1083.126, 1291.366, 1389.144, and 1569.294 peaks/centers in the wavelength region of 4,000.00–650.00 cm−1. The chloroform extract of C. intybus seed and doxorubicin was showed 1411.37 μg/ml and 460.13 μg/ml 50% cell growth inhibition (IC50 against the HCT-15 cell line. Both extract and doxorubicin were safe against the Vero (normal cell line. Conclusion: It can be concluded that the chloroform extract of C. intybus seed was not efficient against the HCT-15 cell line at the concentrations used in the experiment. Furthermore, there is no need to explore the said studies by in vivo models.

  7. A chicory cytochrome P450 mono-oxygenase CYP71AV8 for the oxidation of (+)-valencene

    NARCIS (Netherlands)

    Cankar, K.; Houwelingen, van A.M.M.L.; Bosch, H.J.; Sonke, Th.; Bouwmeester, H.J.; Beekwilder, M.J.

    2011-01-01

    Chicory (Cichorium intybus L.), which is known to have a variety of terpene-hydroxylating activities, was screened for a P450 mono-oxygenase to convert (+)-valencene to (+)-nootkatone. A novel P450 cDNA was identified in a chicory root EST library. Co-expression of the enzyme with a valencene syntha

  8. Effects of Dairy Cattle Manure and Chemical Fertilizers on Fertility of Soils Grown with Cichorium intybus

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    [Objective] The aim was to explore recycling utilization of manure of dairy cattle through returning of manures into fields. [Method] Effects of dairy cattle ma- nure and chemical fertilizer on fertility of soils grown with Cichorium intybus were in- vestigated through a pot experiment. [Result] After manure of dairy cattle was ap- plied, it can be concluded that organic matter, total N, total P, alkali-hydrolyzable ni- trogen, available P, activities of urease and invertase in soils increased by 0.14-1.28 times, 43.8%-79.7%, 17.4%-30.8%, 147%-188%, 7 times, 17.2%-38.5%, and 1.36%- 3.34%, respectively. Furthermore, organic matter, total N. urease and invertase activi- ties in group of M7F3 increased most; total P and available P achieved the best in group of M3F7. These indicated that the applied manures of dairy cattle would maintain and improve soil fertility, providing better soils for Cichorium intybus. [Conclusion] The research provides reference for recycling of cattle manures and construction of ecological cyclical pattern of "grass planting-cattle breeding-methane fermentation-returning of manures into fields".

  9. Cichorium intybus Linn. Extract Prevents Type 2 Diabetes Through Inhibition of NLRP3 Inflammasome Activation.

    Science.gov (United States)

    Shim, Do-Wan; Han, Ji-Won; Ji, Young-Eun; Shin, Woo-Young; Koppula, Sushruta; Kim, Myong-Ki; Kim, Tae-Kweon; Park, Pyo-Jam; Kang, Tae-Bong; Lee, Kwang-Ho

    2016-03-01

    This study provides the scientific basis for the inhibitory effect of the aerial parts of Cichorium intybus Linn. (C. intybus) on the activation of the NLRP3 inflammasome in vitro and on high-fat diet (HFD)-induced type-2 diabetes (T2D). Lipopolysaccharide (LPS)-primed bone marrow-derived macrophages were used to study the effects methanolic extract of C. intybus leaf (CI) on inflammasome activation. An insulin resistance model (mice fed a HFD) was used to study the in vivo effect of CI on T2D. CI attenuated interleukin-1β (IL-1β) secretion by inhibiting the activation of the NLRP3 inflammasome in mouse bone marrow macrophages. The CI treatment attenuated the intracellular movement of NLRP3 in Triton X-100 insoluble fraction, without affecting the expression of other NLRP3 inflammasome-related proteins. Attenuated IL-1β secretion may improve glucose metabolism in the HFD-fed insulin resistance mouse model. CI also attenuated the infiltration of M1 macrophages and increased the M2 macrophage population in white adipose tissue. Collectively, our data showed that CI inhibits IL-1β secretion through attenuation of NLRP3 inflammasome activation, leading to an antidiabetic effect by improving glucose metabolism and inhibiting metainflammation. PMID:26987023

  10. Etude des potentialités de la vision artificielle pour la reconnaissance optique des semences immatures de chicorée industrielle (Cichorium intybus L.

    Directory of Open Access Journals (Sweden)

    Ooms, D.

    2010-01-01

    Full Text Available Study of the potentialities of machine vision used for optical selection of immature seeds of industrial chicory (Cichorium intybus L.. The commercial production of industrial chicory seeds (cypselae includes the postharvest elimination of non-viable seeds by non-destructive tools. For this purpose, two machine vision methods are described for the detection of non-viable seeds: color vision and fluorescence imaging. The analysis of color images of 1,500 seeds of the 'Nausica' variety allows only the recognition of desiccated and undeveloped seeds. This is caused by the large variability of seed color, shape and texture. Fluorescence imaging is set up in order to analyze the repartition of chlorophyll fluorescence, a marker of seed maturity, on different areas of the seed (pericarp, radicle tip and pappus. A very sensitive system is needed due to the low chlorophyll content and the large amount of seeds to be sorted. A fluorescence imaging system is proposed. Its distinctive feature is the possibility to modify the spectrum of the light source (in order to optimize the sensibility of the machine vision system and to record the evolution of fluorescence repartition with time. The system is functional and delivers images of fluorescence repartition within external cypsela tissues. It could allow to analyze the fluorescence of a large sample of seeds to correlate fluorescence features to germinability and maturity.

  11. STUDY ON VARIABILITY OF CICHORIUM INTYBUS L. FLOWERS AND FRUITS

    Directory of Open Access Journals (Sweden)

    Nicolaie Ionescu

    2015-07-01

    Full Text Available Variability studies of weeds in agricultural fields are useful in promoting integrated management measures. It was found that a higher variability of any species can be controlled more easily by various methods. And if C.intybus species was a significant spread in agricultural fields, while variability information they are fewer. Weed, being adapted white luvicsoil the south of the country has shown that inflorescence have diameters between 3 and 5 cm, with a ligulae flower number between 13 and 25 pieces. Their modal values were 3.8-4.0 cm in diameter and 15-16 ligulae flowers number in head (capitula. Fruit s (achene were formed in a number between 1 and 15 in the head, with a length between 1.5 and 2.6 mm, and the width between 0.7 and 1.1 mm. Modal values of these dimensions of fruits were 7-9 pieces/ head, with 1.9-2.0 length and 0.9 mm width. Correlations between the various characters showed positive results, and that means the plant have a good adaptation to the various vegetation conditions.

  12. Sesquiterpene lactones and inulin from chicory roots: extraction, identification, enzymatic release and sensory analysis.

    OpenAIRE

    Leclercq, E.

    1992-01-01

    Chicory ( Cichorium intybus L.) is one of the many species of the family Compositae. Chicory has been cultivated for the production of leaves or chicons, which have been used as a vegetable since approximately 300 BC, and for its roots, which can be used as a coffee substitute after roasting.Chicory leaves are appreciated for their slightly bitter taste. Two bitter compounds were known at the start of this project: lactucin (Lc) and lactucopicrin (Lp), both sesquiterpene lactones with a guaia...

  13. Forage characteristics affecting meat goat preferences for forage chicory cultivars

    Science.gov (United States)

    Concentration of bitter sesquiterpene lactones (SL), lactucin, lactucopicrin, and 8-deoxylactucin, has been associated with low soil phosphorus fertility and reduced livestock preference for forage chicory (Cichorium intybus L.). We evaluated the effect of cultivar and available soil P (ASP) on mea...

  14. Effects of Cichorium Intybus L. Root Extract on Secretory Activity of the Stomach in Health and Ulcer Disease.

    Science.gov (United States)

    Krylova, S G; Vymyatnina, Z K; Zueva, E P; Amosova, E N; Razina, T G; Litvinenko, V I

    2015-09-01

    Gastroprotective effect of Cichorium intybus L. root extract is demonstrated on H. Shay's model of experimental ulcer in rats. The effect is attributed to the antisecretory activity of the plant and stimulation of defense barrier function of the gastric mucosa. The regulatory effect of the phytocomplex on seasonal characteristics of the gastric secretory and defense functions in dogs with Basov's fistula is detected. PMID:26468023

  15. The Potential Health Benefits of Polyphenol-Rich Extracts from Cichorium intybus L. Studied on Caco-2 Cells Model

    OpenAIRE

    Elena Azzini; Giuseppe Maiani; Ivana Garaguso; Angela Polito; Foddai, Maria S.; Eugenia Venneria; Alessandra Durazzo; Federica Intorre; Lara Palomba; Rauseo, Maria L.; Ginevra Lombardi-Boccia; Fabio Nobili

    2016-01-01

    Phytochemicals can exert their bioactivity without reaching the systemic circulation; scarcely absorbed antioxidants might reach the large bowel contributing to protection from oxidative damage-induced gastrointestinal diseases. In the present work, we aimed to study the relationship between potential activity of polyphenol-rich extracts from Cichorium intybus L. and changes in morphological characteristics on Caco-2 cells. Phytochemicals content (carotenoids and flavonoids) and total antioxi...

  16. HPLC fingerprint and chemical pattern recognition method for Cichorium intybus and C.glandulosum%菊苣和毛菊苣的HPLC指纹图谱构建及模式识别研究

    Institute of Scientific and Technical Information of China (English)

    周俊; 张冰; 林志健; 吴丽丽; 朱文静; 孙博喻

    2013-01-01

    Objective:To establish fingerprint analysis method by HPLC for the quality control of Cichorium intybus and effective identification of C.intybus and C.glandulosum.Method:The software "Similarity Evaluation System for Chromatographic Fingerprint of TCMs" (Version 2004A) was employed to generate the mean chromatogram and carry out the similarity analysis of the sampies.Cluster analysis was adopted in combination with principal component analysis to study seventeen chicory's characteristic common peaks and to differentiate the two chicory resources.Result:The fingerprint of C.intybus and C.glandulosum has been set up,and the range of similarity for seventeen chicory samples was 0.847-0.988.The difference among chromatographic fingerprints of chicory samples between the two different varieties was identified by cluster analysis and principal component analysis.Conclusion:The method can be used to evaluate the quahty of C intybus and identify C.intybus and C glandulosum conveniendy.%目的:建立菊苣和毛菊苣地上部分的HPLC指纹图谱,为鉴别菊苣品种和有效控制菊苣质量提供参考.方法:采用高效液相色谱法采集了来自全国14个省市11批菊苣和6批毛菊苣样品的指纹图谱,按“中药色谱指纹图谱相似度评价系统”建立对照图谱,并对样品进行相似度评价.运用SPSS软件对不同品种菊苣的HPLC指纹图谱特征峰进行了聚类分析和主成分分析.结果:成功建立菊苣和毛菊苣指纹图谱,11批菊苣样品相似度均大于0.874,6批毛菊苣样品相似度均大于0.9.聚类分析和主成分分析均实现了菊苣和毛菊苣HPLC指纹图谱差异的识别.结论:该方法简便、可靠,可用于菊苣、毛菊苣的品种鉴别和菊苣的质量评价.

  17. Interactions between dietary chicory, gut microbiota and immune responses

    OpenAIRE

    Liu, Haoyu

    2013-01-01

    This thesis provides a better understanding of interactions between diet, gut microbiota, and immune responses to a specific dietary fiber source, chicory (Cichorium intybus L). This was achieved by examining the impact of chicory fiber on animal performance, digestibility, gut development, commensal bacteria community structure in small and large intestine, and follow-up reactions with specific immune components, cytoprotective heat shock protein (HSP) 27 and 72, in vivo and in vitro. T...

  18. Evaluation of chicory seeds maturity by chlorophyll fluorescence imaging

    OpenAIRE

    Ooms, David; Destain, Marie-France

    2011-01-01

    Chicory (Cichorium intybus L.) seed production includes sorting to remove foreign materials and non-viable seeds. A machine vision system was developed to monitor the fluorescence in order to detect the immature chicory seeds. It comprised a monochromatic light source, a highpass filter and a monochromatic CCD camera sensitive to red and infrared. With this device, blue light reflected by the seeds was blocked whilst red fluorescence was measured by the camera. A segmentation a...

  19. СПЕКТРОСКОПИЧЕСКИЕ СВОЙСТВА ДИКОРАСТУЮЩЕГО ЛЕКАРСТВЕННОГО РАСТЕНИЯ ЦИКОРИЯ ОБЫКНОВЕННОГО (CICHORIUM INTYBUS L.)

    OpenAIRE

    Шукуров, Т.; Рахматова, З.; Марупов, Р.

    2009-01-01

    In present of work the investigation physical-chemical property separates components wildgrowing medicinal plant chicory ordinary (Cichorium intybus L.), by methods IR-spectroscopy. On the basis of research of spectral properties both by results of cation-exchange and on displacement of frequency of a maximum characteristic IR band of absorption, energy of intermolecular interaction is certain, and are shown, that leaves contain greater sugar and pigments, than other components.

  20. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2013. Scientific Opinion on the substantiation of a health claim related to a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf chard, red Swiss chard, golden Swiss chard and

    DEFF Research Database (Denmark)

    Tetens, Inge

    related to a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf chard, red Swiss chard, golden Swiss chard and white Swiss chard and maintenance of normal blood cholesterol concentration. The food that is the subject of the health claim, a combination of red...... spinach (Spinacia oleracea L.), green spinach (Spinacia oleracea L.), red chicory (Cichorium intybus L.), green chicory (Cichorium intybus L.), green leaf chard (Beta vulgaris L. var. cicla), red leaf chard (Beta vulgaris L. var. cicla), red Swiss chard (Beta vulgaris L. var. cicla), golden Swiss chard...... the scientific substantiation of the claim were provided by the applicant. The Panel concludes that a cause and effect relationship has not been established between consumption of a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf chard, red Swiss chard...

  1. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2013. Scientific Opinion on the substantiation of a health claim related to a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf chard, red Swiss chard, golden Swiss chard and

    DEFF Research Database (Denmark)

    Tetens, Inge

    related to a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf chard, red Swiss chard, golden Swiss chard and white Swiss chard and protection of blood lipids from oxidative damage. The food that is the subject of the health claim, a combination of the...... following frozen vegetables: red spinach (Spinacia oleracea L.), green spinach (Spinacia oleracea L.), red chicory (Cichorium intybus L.), green chicory (Cichorium intybus L.), green leaf chard (Beta vulgaris L. var. cicla), red leaf chard (Beta vulgaris L. var. cicla), red Swiss chard (Beta vulgaris L. var...... conclusions could be drawn for the scientific substantiation of the claim were provided by the applicant. The Panel concludes that a cause and effect relationship has not been established between consumption of a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf...

  2. A preliminary study on early biological effects on Cichorium intybus cv. Puna after space mutagenesis in a space satellite%航天搭载菊苣种子的生物学特性研究

    Institute of Scientific and Technical Information of China (English)

    张玉; 白史且; 王曾珍; 李达旭; 邓永昌

    2012-01-01

    Dry seeds of Cichorium intybus were carried by return satellite for space mutagenesis.The space effects on characteristics of chicory SP1,including the germination capacity ability,vigour of germination,the growth of seedlings? main root length,and agronomiv characteristics in the field,were analyzed after space mutagensis.There were significant differences between both chicories and their ground controls.SAG0002 was better than SAG0001.Except for individual characters,the biological effect on different varieties of chicory was significantly promoted.%利用返回式卫星搭载菊苣种子进行空间诱变处理,对航天搭载的2份菊苣SP1代群体的生物学特性进行了分析,主要包括发芽率、发芽势、幼苗生长、田间农艺性状等.结果表明,2份菊苣的生物学特性有一定的差异,SAG0002材料表现优于SAG0001,但除个别性状外,航天搭载从总体上对地上部性状起到一定的促进作用.

  3. Intra- and interspecific chromosome polymorphisms in cultivated Cichorium L. species (Asteraceae)

    OpenAIRE

    Bernardes, Ebenézer C.S.; Benko-Iseppon, Ana M.; Santelmo Vasconcelos; Reginaldo de Carvalho; Brasileiro-Vidal, Ana C.

    2013-01-01

    Endive (Cichorium endivia L.) and chicory (C. intybus L.) both have 2n = 18, but until now, there has been no detailed karyomorphological characterization. The present work evaluated five accessions of each species using FISH with rDNA probes and fluorochrome staining with CMA and DAPI. Both species presented distinct banding patterns after fluorochrome staining: while endive had proximal CMA++/DAPI- bands in the short arms of pairs 1, 2 and 3, chicory had proximal CMA-positive bands in chrom...

  4. In vitro regeneration ability of diploid and autotetraploid plants of Cichorium intybus L.

    Science.gov (United States)

    Ravandi, E Ghotbi; Rezanejad, F; Dehghan, E

    2014-01-01

    Polyploidy has played a significant role in the evolutionary history of plants and is a valuable tool for obtaining useful characteristics. Because of the novelty of polyploids, comparison of their in vitro culture response with diploids would be notable. In this study, leaf explants from diploid, autotetraploid and mixoploid plants of Cichorium intybus L. were cultured in vitro on the similar media and under same conditions. The ploidy level of the obtained calluses and regenerants were determined by flow cytometry analysis. The callogenic response of leaf explants cultured on the callus induction medium did not depend on the ploidy level of their parental plants. According to the flow cytometry analysis, the increased ploidy levels (4x) and (8x) were observed in the callus cultures with diploid and tetraploid origin, respectively. A considerable difference was observed between the ploidy level of mixoploid plants and their calluses, indicating the dominance of diploid cells in the callus tissue. The results showed that polyploidy led to the loss of organogenic potential as the tetraploid origin calluses failed to regenerate, while the diploid origin calluses successfully regenerated to whole plants. PMID:25016827

  5. Response of forage chicory seedlings to available soil phosphorus in two soils in a controlled environment

    Science.gov (United States)

    Differences in growth and chemical composition among forage chicory (Cichorium intybus L.) cultivars grown in WV and PA are possibly related to P fertility of soils. In order to remove the environmental effect, we used silt loam soils from WV and PA to test the effect of available soil P (ASP) on '...

  6. Prevention of oxidative stress-induced apoptosis of C2C12 myoblasts by a Cichorium intybus root extract.

    Science.gov (United States)

    Lee, Yong-Hyeon; Kim, Dae-Hyun; Kim, Yoon Suk; Kim, Tack-Joong

    2013-01-01

    Cell injury associated with reactive oxygen species (ROS) has been reported in various muscular disorders. We found that a Cichorium intybus (Cii) extract reduced H(2)O(2)-induced viability loss in C2C12 myoblasts, inhibited oxidative stress-induced apoptosis and increased intracellular heat shock protein 70 (Hsp 70) expression. Cii also inhibited the level of intracellular ceramide. These results indicate that Cii may prevent skeletal muscle atrophy by inducing the expression of Hsp 70 and inhibiting the level of ceramide. PMID:23391909

  7. X-Ray Diffraction Structure of a plant Glycosyl Hydrolase family 32 protein: Fructan 1-Exohydrolase IIa of Cichorium intybus

    OpenAIRE

    Verhaest, Maureen; Van den Ende, Wim; Le Roy, Katrien; De Ranter, Camiel; Van Laere, André; Rabijns, Anja

    2005-01-01

    Fructan 1-exohydrolase, an enzyme involved in fructan degradation, belongs to the glycosyl hydrolase family 32. The structure of isoenzyme 1-FEH IIa from Cichorium intybus is described at a resolution of 2.35 Å. The structure consists of an N-terminal fivefold β-propeller domain connected to two C-terminal β-sheets. The putative active site is located entirely in the β-propeller domain and is formed by amino acids which are highly conserved within glycosyl hydrolase family 32. The fructan-bin...

  8. Daily Share of Green Matter in Pure or Associated Fodder Chicory

    OpenAIRE

    Dorin Camen; Nicoleta Moraru; Neculai Dragomir; Carmen Dragomir; Ghiocel Claudiu; Sala Anca; Neciu Florin

    2015-01-01

    Chicory (Cichorium intybus L.), used as fodder, has some favourable features: high longevity, resistance to drought and to soil conditions (acidity, alkalinity), cultivable pure or associated with different grassland species, quick recovery after mowing or grazing, high degree of consumability, high nutritive value. Research shows that chicory, sowed pure or in association with legume species or with grassland grasses, produces daily, depending on vegetation duration, important amounts of gre...

  9. In vivo efficacy of chicory silage against parasitic nematodes in cattle

    OpenAIRE

    Pena-Espinoza, Miguel Angel; Desrues, Oliver; Williams, A; S. Thamsborg; Enemark, Heidi

    2014-01-01

    Infections with parasitic nematodes are a persistent risk for grazing cattle, which is met with widespread use of synthetic anthelmintics. However, heavy reliance on these drugs may lead to selection of drug-resistant nematodes. Integrated control strategies, including anti-parasitic crops, are needed. Chicory (Cichorium intybus) can have an impact against sheep nematodes, but this has not been investigated in cattle. Our study aimed to assess the effect of chicory on infections with abomasal...

  10. REGULATION OF PRODUCTION PERFORMANCE OF CHICORY PLANTS BY FOLIAR APPLICATION OF BIOLOGICALLY ACTIVE SUBSTANCES

    OpenAIRE

    MAREK KOVÁR; IVAN ČERNÝ

    2012-01-01

    In this study were evaluated both the growth and yield potentials of three chicory (Cichorium intybus var. sativum) varieties ('Fredonia Nova', 'Oesia' a 'Maurane') growing in natural agro-ecological conditions from 2006 to 2008. Regulation of the crop productivity by foliar application of biologically active substances (Atonik, Polybor 150, and Biafit Gold) was also studied. Evaluation of growth-production performance of chicory was realized as: leaf area index (LAI), photosynthetic potentia...

  11. Role of electrolytes disturbances and Na(+)-K(+)-ATPase in cisplatin - induced renal toxicity and effects of ethanolic extract of Cichorium intybus.

    Science.gov (United States)

    Noori, Shafaq; Mahboob, Tabassum

    2012-10-01

    Cisplatin is known by its toxicity by disturbing electrolytes homeostasis. Thus we aimed to find out the role of herbal plant Cichorium intybus on Cisplatin - induced toxicity. 24 male Albino Wistar rats were randomly divided into 4 groups: Group I is termed as untreated control; Group II is Cisplatin control and received 3 mg/ kg b.w.; i.p.; Group III received C. intybus ethanolic extract at a dose of 500 mg/kg b.w. orally for 10 consecutive days and Group IV is Cisplatin + C. intybus pretreated group. C. intybus is given 30 minutes prior to Cisplatin. Cisplatin - induced electrolytes disturbances is indicated by increase Intra - erythrocyte sodium content, decreased plasma magnesium, calcium and Intra-erythrocyte Na(+)-K(+)-ATPase which implicates the renal toxicity. At a dose of 500 mg/kg b.w. of C. Intybus pretreatment showed partial counter action on the electrolytes imbalances and Na(+)-K(+)-ATPase activity. PMID:23010005

  12. Phytochemical Analysis and Antioxidant Potential of the Phytonutrient-Rich Decoction of Cichorium spinosum and C. intybus.

    Science.gov (United States)

    Brieudes, Vincent; Angelis, Apostolis; Vougogiannopoulou, Konstantina; Pratsinis, Harris; Kletsas, Dimitris; Mitakou, Sofia; Halabalaki, Maria; Skaltsounis, Leandros A

    2016-07-01

    The Cretan diet, as the basis of the Mediterranean diet, has provided traditional remedies for the general well being of people through the long-established consumption of cooked wild greens and vegetables. The intake of the water decoctions of Cichorium spinosum and Cichorium intybus in the context of the daily dietary regime in Greece has been long associated with "liver detoxifying" properties. In the current study, we performed an in-depth investigation of the water decoctions traditionally prepared from C. spinosum and C. intybus through qualitative UHPLC-HRMS profiling and direct quantification of cichoric and caftaric acid as major antioxidant components of the decoction. In addition, we developed a one-step countercurrent chromatography method for the isolation of the two phenolic acids, along with a sulfoconjugate sesquiterpene lactone present only in the Cretan C. spinosum. All water decoctions were found not to be cytotoxic in human fibroblasts, whereas they all significantly reduced the intracellular reactive oxygen species, which is consistent with the major presence of strong antioxidant compounds such as cichoric acid. This work demonstrates that the intake of these decoctions in doses suggested by Greek traditional use is comparable to the ingestion of a phytomedical preparation of antioxidants. These results contribute to our current knowledge on the beneficial health effect of the Cretan diet. PMID:27220071

  13. Performance and carcass parameters when meat goats were finished on chicory, birdsfoot trefoil, or red clover pastures

    Science.gov (United States)

    The meat goat industry is growing rapidly in the eastern U.S., particularly on small farms, to supply ethnic market demands. Body weight (BW), average daily gain (ADG), and carcass parameters were determined when meat goat kids were finished on pastures of chicory (Cichorium intybus L.; CHIC), bird...

  14. Influence of the Forcing Process on Some Qualitative Aspects in Radicchio “Rosso di Treviso Tardivo” (Cichorium Intybus L., Group Rubifolium. 2. Antioxidant Capacity, Phenols and Ascorbic Acid

    Directory of Open Access Journals (Sweden)

    Carlo Nicoletto

    2010-03-01

    Full Text Available There is currently a great deal of interest in phytochemicals as bioactive components of food, especially antioxidant compounds that could benefit human health. Radicchio “Rosso di Treviso tardivo” (Cichorium intybus L., group rubifolium is a vegetable with high antioxidant properties, but nothing is known about antioxidant compounds changes during the forcing process. The experiment was conducted in Veneto (north-eastern Italy, 45°36’N; 12°10’E with plants grown in a loamy soil and analyzed at harvest and during the forcing process (0-10 and 20 forcing days- FD. Results showed that the antioxidant capacity (AOC, phenols and ascorbic acid contents of this chicory decreased with different intensity during the forcing process. The outer inedible leaves displayed high AOC, which could provide phenolic extracts that could be used as natural antioxidants or to functionalize foods. The properties verified so far mean that this chicory can be considered an interesting and healthy vegetable for the consumer, also after the forcing process.

  15. The global optimization of the ethanol extracts from Cichorium intybus root and its herbicidal activity%菊苣根乙醇提取工艺优化及除草活性测定

    Institute of Scientific and Technical Information of China (English)

    刘玉燕; 杜金鸿; 陈果; 王佺珍; 崔健

    2011-01-01

    利用L16(43×26)混合正交考察乙醇体积分数、料液比、浸泡时间、浸泡重复次数、超声温度、超声时间、超声输入功率和超声重复次数8个因素对菊苣(Cichorium intybus)根提取率的影响;以稗草(Echinochloa crusgali)、反枝苋(Amaranthus retroflexus)为研究对象测定菊苣根提取物的除草活性.8个提取因素对提取率影响均显著(P0.05).L16(42×26)混合正交准确优化了8个因素且得出重要性次序,菊苣根粗提物有开发植物源除草剂的潜力.%In order to observe influences of the eight factors, including ethanol content, ratio of solid-tosolvent, impregnating time and replications, ultrasonic temperature and time, input power, and ultrasonic replications, on Chicory root (Cichorium intybus) extractive rate, L16 (43 × 26) mixed orthogonal design was applied in the study. To determine herbicidal activity of Chicory root extracts, Echinochloa crusgalli and Amaranthus retroflexus seeds were geminated in different concentrations of aqueous extracts of Chicory roots. Results of the study revealed that the eight factors influenced the extracting process (P<0.05)significantly. The optimal conditions of the extracting process consisted of that zero of ethanol content,1: 8 of the ratio of solid-to-solvent, 65℃ of ultrasonic temperature, 24 h of impregnating time, 2 times of impregnating replications, 30 min of ultrasonic time, 400 W of ultrasonic input power and 2 times of ultrasonic replications. Besides, the importance of the eight factors were displayed with gradual decrease as ethanol content, impregnating replications, ultrasonic input power, ultrasonic temperature, ultrasonic replications, the ratio of solid-to-solvent, impregnating time and ultrasonic time. The effect of sixteen treatments of Chicory root crude extracts on E. crusgalli and A. retroflexus seed gemination proved that the Chicory root extracts had herbicidal activity and the inhibitory activity was significant

  16. Evaluation of hepatocyteprotective and anti-hepatitis B virus properties of Cichoric acid from Cichorium intybus leaves in cell culture.

    Science.gov (United States)

    Zhang, Hong-Li; Dai, Ling-Hao; Wu, Yi-Hang; Yu, Xiao-Ping; Zhang, Yong-Yong; Guan, Rong-Fa; Liu, Tao; Zhao, Jun

    2014-01-01

    Hepatitis B is the most common serious liver infection in the world. To date, there is still no complete cure for chronic hepatitis B. Natural caffeic acid analogues possess prominent antiviral activity, especially anti-hepatitis B virus (HBV) and anti-human immunodeficiency virus effects. Cichoric acid is a caffeic acid derivative from Cichorium intybus. In the study, the anti-hepatitis B property of cichoric acid was evaluated by the D-galactosamine (D-GalN)-induced normal human HL-7702 hepatocyte injury model, the duck hepatitis B virus (DHBV)-infected duck fetal hepatocytes and the HBV-transfected cell line HepG2.2.15 cells, respectively. The results showed that cichoric acid attenuated significantly D-GalN-induced HL-7702 hepatocyte injury at 10-100 µg/mL and produced a maximum protection rate of 56.26%. Moreover, cichoric acid at 1-100 µg/mL inhibited markedly DHBV DNA replication in infected duck fetal hepatocytes. Also, cichoric acid at 10-100 µg/mL reduced significantly the hepatitis B surface and envelope antigen levels in HepG2.2.15 cells and produced the maximum inhibition rates of 79.94% and 76.41%, respectively. Meanwhile, test compound at 50-100 µg/mL inhibited markedly HBV DNA replication. In conclusion, this study verifies the anti-hepatitis B effect of cichoric acid from Cichorium intybus leaves. In addition, cichoric acid could be used to design the antiviral agents. PMID:24759764

  17. Anthelmintic effects of forage chicory against parasitic nematodes in cattle

    DEFF Research Database (Denmark)

    Pena-Espinoza, Miguel Angel; Williams, Andrew; Thamsborg, Stig Milan;

    BACKGROUND: Chicory (Cichorium intybus) has potential as a natural anthelmintic in livestock, however evidence of efficacy against cattle nematodes is lacking. Here, we investigated anthelmintic effects of chicory in stabled calves. METHODS: Jersey male calves (2-4 months) were stratified by live...... weight and allocated randomly to 2 groups: chicory (CHI, n=9) and control (CON, n=6). CHI and CON calves were fed with forage chicory silage (cv. Spadona) and hay ad libitum, resp., for 8 weeks. After 2 weeks, calves were infected with 10,000 Ostertagia ostertagi and 65,000 Cooperia oncophora larvae....... Fecal egg counts (FEC) and live weights were assessed weekly. Six weeks after infection, calves were slaughtered for worm recovery. In parallel, total sesquiterpene lactone (SL)-extracts from forage chicory (Spadona and cv. Puna II) were prepared and incubated with first-stage larvae (L1) of O...

  18. Influence of the forcing process on some qualitative aspects in radicchio “Rosso di Treviso tardivo” (Cichorium intybus L., group rubifolium). 1. Nitrate, nitrite and organic nitrogen

    OpenAIRE

    Carlo Nicoletto; Ferdinando Pimpini

    2009-01-01

    Radicchio “Rosso di Treviso tardivo” (Cichorium intybus L., group rubifolium), a typical vegetable of north-eastern Italy, has gained increasing commercial interest in recent years due to its particular shape and culinary features. These properties are obtained by a forcing process that could affect nitrate and dangerous nitrite contents in the edible product. The experiment was conducted in Veneto (north-eastern Italy, 45°36’N; 12°10’E) with plants grown in a ...

  19. Preventive effect of Cichorium intybus L. two extracts on cerulein-induced acute pancreatitis in mice

    Directory of Open Access Journals (Sweden)

    Mohsen Minaiyan

    2012-01-01

    Conclusions: These data suggest that C. intybus hydroalcoholic extracts were effective to protect against experimental acute pancreatitis and the efficacy was partly dependent to the dose and was more significant after parenteral administration.

  20. CHARACTERISATION OF INULIN FROM CHICORY AND SALSIFY CULTIVATED IN PORTUGAL

    OpenAIRE

    M. L. BEIRÃO-DA-COSTA; M. I.N. JANUÁRIO; F. M.S. SIMÃO; A. E.B. LEITÃO

    2009-01-01

    Inulin and fructooligosaccharides (FOS) are important ingredients used in the food industry because of their diverse nutritional and functional properties. Among the sources of these compounds, chicory (Cichorium intybus L.) is one of the most important, due to its high content of inulin, which shows a high F/G ratio. As in several European countries, chicory is grow...

  1. Loss of function of 1-FEH IIb has more impact on post-harvest inulin degradation in Cichorium intybus than copy number variation of its close paralog 1-FEH IIa.

    Science.gov (United States)

    Dauchot, Nicolas; Raulier, Pierre; Maudoux, Olivier; Notté, Christine; Draye, Xavier; Van Cutsem, Pierre

    2015-01-01

    Key Message: The loss of mini-exon 2 in the 1-FEH IIb glycosyl-hydrolase results in a putative non-functional allele. This loss of function has a strong impact on the susceptibility to post-harvest inulin depolymerization. Significant variation of copy number was identified in its close paralog 1-FEH IIa, but no quantitative effect of copy number on carbohydrates-related phenotypes was detected. Inulin polyfructan is the second most abundant storage carbohydrate in flowering plants. After harvest, it is depolymerized by fructan exohydrolases (FEHs) as an adaptive response to end-season cold temperatures. In chicory, the intensity of this depolymerization differs between cultivars but also between individuals within a cultivar. Regarding this phenotypic variability, we recently identified statistically significant associations between inulin degradation and genetic polymorphisms located in three FEHs. We present here new results of a systematic analysis of copy number variation (CNV) in five key members of the chicory (Cichorium intybus) GH32 multigenic family, including three FEH genes and the two inulin biosynthesis genes: 1-SST and 1-FFT. qPCR analysis identified a significant variability of relative copy number only in the 1-FEH IIa gene. However, this CNV had no quantitative effect. Instead, cloning of the full length gDNA of a close paralogous sequence (1-FEH IIb) identified a 1028 bp deletion in lines less susceptible to post-harvest inulin depolymerization. This region comprises a 9 bp mini-exon containing one of the three conserved residues of the active site. This results in a putative non-functional 1-FEH IIb allele and an observed lower inulin depolymerization. Extensive genotyping confirmed that the loss of mini-exon 2 in 1-FEH IIb and the previously identified 47 bp duplication located in the 3'UTR of 1-FEH IIa belong to a single haplotype, both being statistically associated with reduced susceptibility to post-harvest inulin depolymerization

  2. Loss of function of 1-FEH IIb has more impact on post-harvest inulin degradation in Cichorium intybus than copy number variation of its close paralog 1-FEH IIa.

    Directory of Open Access Journals (Sweden)

    Nicolas eDauchot

    2015-06-01

    Full Text Available Inulin polyfructan is the second most abundant storage carbohydrate in flowering plants. After harvest, it is depolymerized by fructan exohydrolases (FEHs as an adaptive response to end-season cold temperatures. In chicory, the intensity of this depolymerization differs between cultivars but also between individuals within a cultivar. Regarding this phenotypic variability, we recently identified statistically significant associations between inulin degradation and genetic polymorphisms located in three fructan exohydrolases. We present here new results of a systematic analysis of copy number variation (CNV in five key members of the chicory (Cichorium intybus GH32 multigenic family, including three FEH genes and the two inulin biosynthesis genes: 1-SST and 1-FFT. qPCR analysis identified a significant variability of relative copy number only in the 1-FEH IIa gene. However, this CNV had no quantitative effect. Instead, cloning of the full length gDNA of a close paralogous sequence (1-FEH IIb identified a 1028 bp deletion in lines less susceptible to post-harvest inulin depolymerization. This region comprises a 9 bp mini-exon containing one of the three conserved residues of the active site. This results in a putative non-functional 1-FEH IIb allele and an observed lower inulin depolymerization. Extensive genotyping confirmed that the loss of mini-exon 2 in 1-FEH IIb and the previously identified 47 bp duplication located in the 3’UTR of 1-FEH IIa belong to a single haplotype, both being statistically associated with reduced susceptibility to post-harvest inulin depolymerization. Emergence of these haplotypes is discussed.

  3. Fructan biosynthesis in crop plants : the molecular regulation of fructan biosynthesis in chicory (Cichorium intybus L.)

    NARCIS (Netherlands)

    Arkel, van J.

    2013-01-01

    Fructan is a polymer of fructose produced by plants and microorganisms. Within the plant kingdom about 45.000 species accumulate fructan as storage carbohydrate in addition to, or instead of, starch. Fructan accumulating species are mainly found in temperate and sub-tropical regions with seasonal or

  4. Fructan biosynthesis in crop plants : the molecular regulation of fructan biosynthesis in chicory (Cichorium intybus L.)

    OpenAIRE

    Arkel, van, J.

    2013-01-01

    Fructan is a polymer of fructose produced by plants and microorganisms. Within the plant kingdom about 45.000 species accumulate fructan as storage carbohydrate in addition to, or instead of, starch. Fructan accumulating species are mainly found in temperate and sub-tropical regions with seasonal or sporadic rainfall. During the last decades, the use of fructan in the (food) industry has rapidly evolved, because of its health promoting characteristics and interesting functional properties.Chi...

  5. [Effects of Eucalyptus grandis leaf litter at its early stage of decomposition on the growth and photosynthetic characteristics of Cichorium intybus].

    Science.gov (United States)

    Wu, Xiu-Hua; Li, Yi-Qiao; Hu, Ting-Xing; Chen, Bao-Jun; Yang, Yong-Gui; Chen, Hong; Hu, Hong-Ling

    2013-07-01

    From March to May, 2010, a pot experiment was conducted to investigate the effects of Eucalyptus grandis leaf litter at its early stage of decomposition on the growth and photosynthetic characteristics of Cichorium intybus. Four treatments with different application rate of the leaf litter, i.e., 0 g x pot(-1) (CK), 30 g x pot(-1) (A1), 60 g x pot(-1) (A2), and 90 g x pot(-1) (A3), were installed. Each pot contained 12 kg soil mixed with the leaf litter, and then, C. intybus was sown. The growth indicators of the C. intybus were measured at the 30, 45, 60, and 75 d after sowing, and the photosynthetic characteristics of the C. intybus in treatment A3 were studied after the seedlings third leaf fully expanded. At each measured time, the biomass accumulation and leaf area growth of C. intybus in treatments A1, A2, and A3 were inhibited significantly. At the early stage of the leaf litter decomposition, the synthesis of photosynthetic pigments of the C. intybus seedlings was inhibited significantly, and the inhibition effect was getting stronger with the increasing amount of the leaf litter addition. The diurnal change of the seedlings photosynthetic rate in all treatments showed a bimodal curve with midday depression, the stomatal conductance and water use efficiency had the same variation trend with the net photosynthetic rate, and the total diurnal photosynthesis decreased in the order of CK > A1 > A2 > A3. The GC-MS analysis showed there were 33 kinds of small molecule compounds released gradually with the decomposition of the leaf litter, among which, allelopathic substance terpenoid dominated. PMID:24175509

  6. Hepatoprotective effect of methanol extracts of Zingiber officinale and Cichorium intybus

    Directory of Open Access Journals (Sweden)

    Atta A

    2010-01-01

    Full Text Available The present work was carried out to investigate the hepatoprotective effect of ginger, chicory and their mixture against carbon tetrachloride intoxication in rats. Carbon tetrachloride treatment significantly elevated the alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and gamma glutamyltransferase activities and the serum triglycerides and cholesterol concentration as compared to control group. It also increased RBCs counts and Hb concentration, total or differential leucocytes counts. However it decreased platelet counts, platelet distribution width, mean platelet volume, platelet larger cell ratio. Methanol extract of ginger (250 and 500 mg/kg and chicory (250 and 500 mg/kg given alone or mixed (1:1 wt/wt significantly restored the carbon tetrachloride-induced alterations in the biochemical and cellular constituents of blood. No toxic symptoms were reported in doses up to 5 g/kg. Alkaloids and/or nitrogenous bases, carbohydrates and/or glycosides, tannins, flavonoids, saponins and unsaturated sterols and/or triterpenes are the main active constituents of their methanol extract. The hepatoprotective effect of ginger and chicory was also confirmed by the histopathological examination of liver tissue.

  7. Estudio de la composición química de la semilla y aceite seminal de Cichorium intybus L. (Achicoria)

    OpenAIRE

    S. M. Nolasco; Quiroga, O.; Wiese, B.; Vigo, M. S.

    1996-01-01

    Seeds from Cichorium intybus L harvested at Olavarría (Buenos Aires, Argentine) were defatted with petroleum ether (60°-80°) and the raw oil obtained with a yield of 13.3% dry basis. The crude oil was examined in their physicochemical characteristics had refractive index of 1.4576 (at 25°), iodine value of 124.3, saponification index of 191,4, unsaponifiable matter of 5.3 % and free fatty acid content of 6.0 (mg KOH/g). Gas chromatographic analysis of the oil revealed high ...

  8. Effect of Season and Seeding Methods on Growth Characteristics of Cichorium intybus cv. Europe%播种季节与播种方式对欧洲菊苣生长特性的影响

    Institute of Scientific and Technical Information of China (English)

    梁万鹏; 梁雪; 赫春杰; 高钰; 彭超

    2015-01-01

    在裂区设计下,以播种季节作为主区处理,播种方式作为副区处理,研究了欧洲菊苣的生长特性指标。结果表明:分蘖数是由品种本身决定的,与播种方式及播种季节无关,播种季节只对株高、产量具有显著影响,播种方式对株高、茎粗、密度、盖度及产量均有显著影响,播种季节与播种方式的交互作用对6个指标均没有显著性的影响。通过对欧洲菊苣各生长指标分别进行播种季节和播种方式的效应分析、多重比较分析,明确了影响欧洲菊苣生长特性的因素,这一分析方法对相似试验具有参考价值。%Under the cleft district design,mainly sowing season for area treatment,seeding method as deputy district, determination of Europe chicory(Cichorium intybus CV. Europe)growth characteristic index. The result shows that the tiller number is decided by the seed itself,has nothing to do with seeding method and sowing season,sowing season only has significant effect on plant height,yield,seeding method for plant height,stem diameter,density,coverage and yield are significant,the interaction of the sowing season and seeding methods for six indicators are no significant influence. Through to Europe chicory the growth index respectively to analyze the effect of the sowing season and multiple comparison analysis,and clear the influencing factors of Europe chicory growth characteristics,this analysis method has reference value to the similar test.

  9. Effect of modified atmosphere applied to minimally processed radicchio (Cichorium intybus L. submitted to different sanitizing treatments

    Directory of Open Access Journals (Sweden)

    Giuliana de Moura Pereira

    2014-09-01

    Full Text Available Stability of minimally processed radicchio (Cichorium intybus L. was evaluated under modified atmosphere (2% O2, 5% CO2, and 93% N2 on 3, 5, 7 and 10 days of storage at 5°C. The samples were hygienized in sodium hypochlorite or hydrogen peroxide solutions to identify the most effective sanitizing solution to remove microorganisms. Microbiological analysis was conducted to identify the presence of coliforms at 35°C and 45°C, mesophilic microorganisms, and yeast and mold. Physicochemical analyses of mass loss, pH, soluble solids, and total acidity were conducted. The color measurements were performed using a Portable Colorimeter model CR-400. The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic methods. The sensory evaluation was carried out using a hedonic scale to test overall acceptance of the samples during storage. The sodium hypochlorite (150 mg.L-1 solution provided greater safety to the final product. The values of pH ranged from 6.17 to 6.25, total acidity from 0.405 to 0.435%, soluble solids from 0.5 to 0.6 °Brix, mass loss from 1.7 to 7.2%, and chlorophyll from 1.068 to 0.854 mg/100g. The antioxidant activity of radicchio did not show significant changes during the first 3 days of storage. The overall acceptance of the sample stored in the sealed package without modified atmosphere was 70%, while the fresh sample was obtained 77% of approval. Although the samples packaged under modified atmosphere had a higher acceptance score, the samples in sealed packages had satisfactory results during the nine days of storage. The use of modified atmosphere, combined with cooling and good manufacturing practices, was sufficient to prolong the life of minimally processed radicchio, Folha Larga cultivar, for up to ten days of storage.

  10. CHARACTERISATION OF INULIN FROM CHICORY AND SALSIFY CULTIVATED IN PORTUGAL

    OpenAIRE

    M. L. BEIRÃ?O-DA-COSTA; M. I.N. JANUÃRIO; F. M.S. SIMÃ?O; A. E.B. LEITÃ?O

    2009-01-01

    Inulin and fructooligosaccharides (FOS) are important ingredients used in the food industry because of their diverse nutritional and functional properties. Among the sources of these compounds, chicory (Cichorium intybus L.) is one of the most important, due to its high content of inulin, which shows a high F/G ratio. As in several European countries, chicory is grow...

  11. Daily Share of Green Matter in Pure or Associated Fodder Chicory

    Directory of Open Access Journals (Sweden)

    Dorin Camen

    2015-05-01

    Full Text Available Chicory (Cichorium intybus L., used as fodder, has some favourable features: high longevity, resistance to drought and to soil conditions (acidity, alkalinity, cultivable pure or associated with different grassland species, quick recovery after mowing or grazing, high degree of consumability, high nutritive value. Research shows that chicory, sowed pure or in association with legume species or with grassland grasses, produces daily, depending on vegetation duration, important amounts of green matter, i.e. between 120 and 280 kg/day/ha without or with nitrogen fertilisation (N150.

  12. Effect of chicory seed extract on glucose tolerance test (GTT) and metabolic profile in early and late stage diabetic rats

    OpenAIRE

    Ghamarian Abdolreza; Abdollahi Mohammad; Su Xiaogang; Amiri Azita; Ahadi Ali; Nowrouzi Azin

    2012-01-01

    Abstract Background and purpose of the study The goal was to evaluate and compare the effects of aqueous extract of the seeds of chicory, Cichorium intybus L., on glucose tolerance test (GTT) and blood biochemical indices of experimentally-induced hyperglycemic rats. Methods Late stage and early stage of Type 2 diabetes mellitus (T2DM) were induced in rats by streptozotocin (STZ) and a combination of STZ and niacinamide (NIA/STZ), respectively. Within each group, one subgroup received daily i...

  13. Pretreatments, conditioned medium and co-culture increase the incidence of somatic embryogenesis of different Cichorium species

    OpenAIRE

    Couillerot, Jean-Paul; Windels, David; Vazquez, Franck; Michalski, Jean-Claude; Hilbert, Jean-Louis; Blervacq, Anne-Sophie

    2012-01-01

    Somatic embryogenesis (SE) in Cichorium involves dedifferentiation and redifferentiation of single cells and can be induced by specific in vitro culture conditions. We have tested the effect of various treatments on the incidence of SE (ISE) of an interspecific embryogenic hybrid (C. endivia x C. intybus) and of different commercial chicories (C. endivia and C. intybus) that are typically recalcitrant to SE in standard culture conditions. We found that the ISE of the hybrid is significantly i...

  14. Reduced sexual compatibility between cultivated and wild chicory and their F1 hybrids

    DEFF Research Database (Denmark)

    Hauser, T.P.; Bagger Jørgensen, Rikke; Toneatto, F.

    2012-01-01

    Crops were domesticated from wild plants not too long ago and have subsequently diverged from the wild ones, especially in traits used by humans. Whether divergence between the cultigen and wild forms has also lead to reduced reproductive compatibility is unknown for many species. Chicory...... (Cichorium intybus L.) has been bred as a crop at least since Roman times. To test if this has led to a loss in reproductive compatibility with wild chicory, we planted cultivar, wild, and F1 hybrid plants into two field plots, and let them pollinate freely. On 2 days, in the beginning and middle of the...... wild chicory. Hybrid plants fathered more seeds than expected on some of the hybrid plants, indicating that hybrids do not suffer much from outbreeding depression. Our study thus suggests that cultivated and wild chicory, even though they belong to the same species, have diverged to an extent where...

  15. A chicory cytochrome P450 mono-oxygenase CYP71AV8 for the oxidation of (+)-valencene

    OpenAIRE

    Cankar, K.; Houwelingen, van, K.; Bosch, H.J.; Sonke, Th.; Bouwmeester, H.J.; Beekwilder, M.J.

    2011-01-01

    Chicory (Cichorium intybus L.), which is known to have a variety of terpene-hydroxylating activities, was screened for a P450 mono-oxygenase to convert (+)-valencene to (+)-nootkatone. A novel P450 cDNA was identified in a chicory root EST library. Co-expression of the enzyme with a valencene synthase in yeast, led to formation of trans-nootkatol, cis-nootkatol and (+)-nootkatone. The novel enzyme was also found to catalyse a three step conversion of germacrene A to germacra-1(10),4,11(13)-tr...

  16. Etude des potentialités de la vision artificielle pour la reconnaissance optique des semences immatures de chicorée industrielle (Cichorium intybus L.)

    OpenAIRE

    Ooms, David; Destain, Marie-France

    2010-01-01

    La production commerciale de semences de chicorée industrielle (Cichorium intybus L.) implique l'élimination des semences (cypsèles) non viables de la récolte à l’aide de méthodes non destructives. Dans ce cadre, deux techniques basées sur la vision artificielle sont étudiées pour reconnaître les semences non viables : la vision couleur et l’imagerie fluorescente. L’analyse de 1500 semences de la variété Nausica en vision en couleur ne permet de détecter que les semences desséchées ou non dév...

  17. Effects of Cichorium intybus linn on blood glucose, lipid constituents and selected oxidative stress parameters in streptozotocin-induced diabetic rats.

    Science.gov (United States)

    Samarghandian, Saeed; Borji, Abasalt; Tabasi, Seyed Hidar

    2013-12-01

    The efficacy of herbal medicine has been confirmed in treatment of diabetes mellitus (DM) by amelioration of oxidative stress. The present study was designed to investigate protective effects of Cichorium intybus extract (CIE) against oxidative damage in diabetic rats. In this study, the rats were divided into the control (C), diabetic (D), D + CIE- treated (125 mg/kg/day) groups. Male Sprague-Dawley rats aged 9 weeks (160 ± 15 g) were administered with streptozotocin (STZ, 60 mg/kg) intraperitoneally (ip) to induce experimental diabetes. From 3 days after STZ administration to the end of the study (4 weeks) the ethanolic extract of CIE was administered (i.p) to diabetic rats. Body weight and blood glucose were measured weekly. At the end of the 4-week period, blood was drawn for biochemical assay, in order to determine the changes of cellular antioxidant defense system, serum oxidative damage and serum lipid were measured profile. CIE injection to diabetic rats resulted in significant reduction in blood glucose, triglyceride (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) levels and significant elevation high density lipoprotein cholesterol (HDL-C) level as well as increase in the body weight as compared with the rats treated with STZ alone. In the treated diabetic group, we also observed the significant increase in reduced glutathione (GSH), superoxide dismutase (SOD), glutathione-S-transferase (GST) and catalase (CAT) with decline in malondialdehyde (MDA) level compared with the non-treated diabetic group. These results suggest that the Cichorium intybus extract has antioxidant properties and prevents diabetes complications by modulation of oxidative stress system. PMID:24304233

  18. Influence of the Forcing Process on Some Qualitative Aspects in Radicchio “Rosso di Treviso Tardivo” (Cichorium Intybus L., Group Rubifolium). 2. Antioxidant Capacity, Phenols and Ascorbic Acid

    OpenAIRE

    Carlo Nicoletto; Ferdinando Pimpini

    2010-01-01

    There is currently a great deal of interest in phytochemicals as bioactive components of food, especially antioxidant compounds that could benefit human health. Radicchio “Rosso di Treviso tardivo” (Cichorium intybus L., group rubifolium) is a vegetable with high antioxidant properties, but nothing is known about antioxidant compounds changes during the forcing process. The experiment was conducted in Veneto (north-eastern Italy, 45°36’N; 12°10’E) with plants g...

  19. A chicory cytochrome P450 mono-oxygenase CYP71AV8 for the oxidation of (+)-valencene.

    Science.gov (United States)

    Cankar, Katarina; van Houwelingen, Adèle; Bosch, Dirk; Sonke, Theo; Bouwmeester, Harro; Beekwilder, Jules

    2011-01-01

    Chicory (Cichorium intybus L.), which is known to have a variety of terpene-hydroxylating activities, was screened for a P450 mono-oxygenase to convert (+)-valencene to (+)-nootkatone. A novel P450 cDNA was identified in a chicory root EST library. Co-expression of the enzyme with a valencene synthase in yeast, led to formation of trans-nootkatol, cis-nootkatol and (+)-nootkatone. The novel enzyme was also found to catalyse a three step conversion of germacrene A to germacra-1(10),4,11(13)-trien-12-oic acid, indicating its involvement in chicory sesquiterpene lactone biosynthesis. Likewise, amorpha-4,11-diene was converted to artemisinic acid. Surprisingly, the chicory P450 has a different regio-specificity on (+)-valencene compared to germacrene A and amorpha-4,11-diene. PMID:21115006

  20. Relationship between High-Performance Liquid Chromatography Fingerprints and Uric Acid-Lowering Activities of Cichorium intybus L.

    OpenAIRE

    Chun-Sheng Zhu; Bing Zhang; Zhi-Jian Lin; Xue-Jie Wang; Yue Zhou; Xiao-Xia Sun; Ming-Liang Xiao

    2015-01-01

    This study aimed to explore the spectrum-effect relationships between high-performance liquid chromatography fingerprints and the uric acid-lowering activities of chicory. Chemical fingerprints of chicory samples from ten different sources were determined by high-performance liquid chromatography, and then investigated by similarity analysis and hierarchical clustering analysis. Pharmacodynamics experiments were conducted in animals to obtain the uric acid-lowering activity information of ea...

  1. Relationship between High-Performance Liquid Chromatography Fingerprints and Uric Acid-Lowering Activities of Cichorium intybus L.

    Science.gov (United States)

    Zhu, Chun-Sheng; Zhang, Bing; Lin, Zhi-Jian; Wang, Xue-Jie; Zhou, Yue; Sun, Xiao-Xia; Xiao, Ming-Liang

    2015-01-01

    This study aimed to explore the spectrum-effect relationships between high-performance liquid chromatography fingerprints and the uric acid-lowering activities of chicory. Chemical fingerprints of chicory samples from ten different sources were determined by high-performance liquid chromatography, and then investigated by similarity analysis and hierarchical clustering analysis. Pharmacodynamics experiments were conducted in animals to obtain the uric acid-lowering activity information of each chicory sample. The spectrum-effect relationships between chemical fingerprints and the uric acid-lowering activities of chicory were established by canonical correlation analysis. The structures of potential effective peaks were identified by liquid chromatography with tandem mass spectrometry. The results showed that a close correlation existed between the spectrum and effect of chicory. Aesculin, chlorogenic acid, chicoric acid, isochlorogenic acid A/B/C and 13,14-seco-stigma5(6),14(15)-diene-3α-ol might be the main effective constituents. This work provides a general model of the combination of high-performance liquid chromatography and uric acid-lowering activities to study the spectrum-effect relationships of chicory, which can be used to discover the principle components responsible for the bioactivity. PMID:26007193

  2. Estudio de la composición química de la semilla y aceite seminal de Cichorium intybus L. (Achicoria

    Directory of Open Access Journals (Sweden)

    Nolasco, S. M.

    1996-12-01

    Full Text Available Seeds from Cichorium intybus L harvested at Olavarría (Buenos Aires, Argentine were defatted with petroleum ether (60°-80° and the raw oil obtained with a yield of 13.3% dry basis. The crude oil was examined in their physicochemical characteristics had refractive index of 1.4576 (at 25°, iodine value of 124.3, saponification index of 191,4, unsaponifiable matter of 5.3 % and free fatty acid content of 6.0 (mg KOH/g. Gas chromatographic analysis of the oil revealed high levels of linoleic acid (59.8%, nearly 21% of saturated acids.
    The residual seed meal contained 16.9% of crude protein, with a low value of available lysine (1.37g/16 g N. Total and phytic acid phosphorous, calcium, ash, crude fiber, sugar, polysaccharides (non presence of starch and residual lipids contents are reported.

    Semillas de Cichorium intybus L (Achicoria cosechadas en Olavarría (prov. de Buenos Aires, Argentina se agotaron con éter de petróleo (60°-80°, obteniendo el aceite crudo con un rendimiento del 13,3% (base seca. El aceite crudo se examinó en sus características fisicoquímicas (índice de refracción: 1.4576 (a 25°, índice de iodo: 124.3, índice de saponificación: 191.4, insaponificable: 5.3%, índice de acidez: 6.0 (mgKOH/g y composición acídica. El análisis por cromatografía gaseosa de los esteres metílicos reveló un alto porcentaje de ácido linoleico (59,8%, alrededor de un 21% de ácidos saturados.
    La harina residual de extracción presentó un 16.9% b.s. de proteína cruda, con un bajo contenido en lisina disponible de 1,37 g/16 g N. Se informan valores de fósforo total y de ácido fítico, cenizas, calcio, fibra cruda, hidratos de carbono y lípidos residuales.

  3. Suitability of Cichorium intybus cultivars in Panxian County, Guizhou Province%贵州省盘县菊苣引种试验

    Institute of Scientific and Technical Information of China (English)

    余昌培; 秦家秀; 党兴静; 颜俤

    2011-01-01

    对将军和普那2个菊苣(Cichorium intybus)品种在贵州盘县3个试验点进行品比试验,分别对物候期、产草量及营养成分等进行观察测定。结果表明,2个菊苣品种在盘县高海拔地区能完成整个生育周期,利用时间长,越冬率和越夏率在75%以上,抗病虫害强;品种将军的产草量高于品种普那,其中鲜草产量比普那高14.99%,干草产量比普那高19.26%;营养价值方面将军表现也优于普那。试验得出,品种将军较适合在贵州盘县地区推广利用。%A field experiment was conducted to determine the suitability of chicoryCichorium intybus) cultivars (Commander, Puna) by measuring the phenophase, forage yield and nutritional ingredient at three points in Panxian County. The results of this study showed that the 2 introduced cultivars performed well at the local environment, indicating that they completed the whole growth cycle with over 75% of winter and summer surviving rate and showed a strongly resistant pest. The forage yield and quality of Commander was higher than that of Puna, indicating that the fresh yield and the dry matter yield of Commander was higher than that of Puna with 14.99% and 19.20%, respectively. This study suggested that the Commander chicory was considered as the high quality and high production forage, which could be extended in the Panxian County, Guizhou Province.

  4. Long-term cultured hairy roots of chicory-a rich source of hydroxycinnamates and 8-deoxylactucin glucoside.

    Science.gov (United States)

    Malarz, Janusz; Stojakowska, Anna; Kisiel, Wanda

    2013-12-01

    A 12-year-old hairy root culture of Cichorium intybus L., a callus culture of the plant as well as roots and leaves of a wild plant of chicory, and roots of two C. intybus L. var. sativum cultivars were examined in respect of their hydroxycinnamate and sesquiterpene lactone compositions and contents. Total phenolics and diphenylpicrylhydrazyl radical scavenging activity of the examined plant tissues were also analyzed. The most active in radical scavenging were extracts from the hairy roots and leaves of chicory. 3,5-Dicaffeoylquinic acid was the major antioxidant present in the hairy roots. Its content in the root biomass reached 5.5 %, calculated on a dry weight basis. 8-Deoxylactucin glucoside (crepidiaside A) was the major sesquiterpene lactone in the hairy roots. Its content reached 1.4 %, calculated on a dry weight basis, and was nearly two orders of magnitude higher than that in the roots of wild chicory plant. The glucosidic derivative of 8-deoxylactucin constituted over 85 % of the total sesquiterpene lactone content in the long-term cultured hairy roots of chicory. Aglycone of this compound was reported to possess anti-inflammatory activity. The qualitative and quantitative analyses of hydroxycinnamates in callus and hairy root cultures of C. intybus were undertaken for the first time. PMID:23975347

  5. Selection and validation of reference genes for quantitative real-time PCR analysis of gene expression in Cichorium intybus.

    Science.gov (United States)

    Delporte, Marianne; Legrand, Guillaume; Hilbert, Jean-Louis; Gagneul, David

    2015-01-01

    Plant polyphenols represent a huge reservoir of bioactive compounds. Industrial chicory, an important crop from northwestern Europe, accumulates an original combination of such compounds, i.e., chlorogenic, isochlorogenic, caftaric, and chicoric acids arising from the phenylpropanoid pathway. For a complete understanding of these biochemical pathways, analyses of gene expression using quantitative real-time PCR (qRT-PCR) should be considered. Because cell cultures are a model of choice for specialized metabolism investigations, this study described for the first time the validation of reference genes for this system in chicory. Eighteen potential reference genes were obtained by mining expressed sequence tag databases of chicory for orthologs of Arabidopsis thaliana genes currently used as reference genes. Twelve genes passed the qRT-PCR standard requirements and their expression stability across different samples was tested using three distinct softwares: geNorm, NormFinder, and BestKeeper. In cell cultures grown under various conditions, TIP41 (TIP41 like protein) was shown to be the most stable gene. Further validation of the proposed reference genes was done by normalization of expression levels of a group of genes of interest. In order to assess the potentiality of the proposed list of candidate reference genes, theses genes were in parallel tested on another experimental design, i.e., chicory seedlings. In this case, the best reference gene identified was Clath (Clathrin adaptator complex subunit). The results highlight the importance of the use of properly validated reference genes to achieve relevant interpretation of qRT-PCR analyses. Here, we provide a list of reference genes suitable for future gene expression studies in chicory. PMID:26347767

  6. Selection and validation of reference genes for quantitative real-time PCR analysis of gene expression in Cichorium intybus

    Directory of Open Access Journals (Sweden)

    Marianne eDelporte

    2015-08-01

    Full Text Available Plant polyphenols represent a huge reservoir of bioactive compounds. Industrial chicory, an important crop from northwestern Europe, accumulates an original combination of such compounds i.e. chlorogenic, isochlorogenic, caftaric and chicoric acids arising from the phenylpropanoid pathway. For a complete understanding of these biochemical pathways, analyses of gene expression using quantitative real-time PCR (qRT-PCR should be considered. Because cell cultures are a model of choice for secondary metabolism investigations, this study described for the first time the validation of reference genes for this system in chicory. Eighteen potential reference genes were obtained by mining expressed sequence tag databases of chicory for orthologs of Arabidopsis thaliana genes currently used as reference genes. Twelve genes passed the qRT-PCR standard requirements and their expression stability across different samples was tested using three distinct softwares: geNorm, NormFinder and BestKeeper. In cell cultures grown under various conditions, TIP41 (TIP41 like protein was shown to be the most stable gene. Further validation of the proposed reference genes was done by normalization of expression levels of a group of genes of interest. In order to assess the potentiality of the proposed list of candidate reference genes, theses genes were in parallel tested on another experimental design i.e. chicory seedlings. In this case, the best reference gene identified was Clath (Clathrin adaptator complex subunit. The results highlight the importance of the use of properly validated reference genes to achieve relevant interpretation of qRT-PCR analyses. Here, we provide a list of reference genes suitable for future gene expression studies in chicory.

  7. Influence of the forcing process on some qualitative aspects in radicchio “Rosso di Treviso tardivo” (Cichorium intybus L., group rubifolium. 1. Nitrate, nitrite and organic nitrogen

    Directory of Open Access Journals (Sweden)

    Carlo Nicoletto

    Full Text Available Radicchio “Rosso di Treviso tardivo” (Cichorium intybus L., group rubifolium, a typical vegetable of north-eastern Italy, has gained increasing commercial interest in recent years due to its particular shape and culinary features. These properties are obtained by a forcing process that could affect nitrate and dangerous nitrite contents in the edible product. The experiment was conducted in Veneto (north-eastern Italy, 45°36’N; 12°10’E with plants grown in a loamy soil and analyzed at harvest and during the forcing process (0-10 and 20 forcing days-FD. Results showed that nitrate and nitrite contents in edible portions never exceed the most restrictive EU limits. A continuous reduction in NO3 content was observed during the forcing process, while NO2 showed a peak at 10 FD. Organic N significantly increased during the forcing process due to NO2 reduction. From a nutritional point of view these results further demonstrate that this type of radicchio is a healthy vegetable for the consumers.

  8. Comparative study of two purified inulinases from thermophile Thielavia Terrestris NRRL 8126 and mesophile Aspergillus Foetidus NRRL 337 grown on Cichorium Intybus l

    Directory of Open Access Journals (Sweden)

    Eman Mohamed Fawzi

    2011-06-01

    Full Text Available Thirty fungal species grown on Cichorium intybus L. root extract as a sole carbon source, were screened for the production of exo-inulinase activities. The thermophile Thielavia terrestris NRRL 8126 and mesophile Aspergillus foetidus NRRL 337 gave the highest production levels of inulinases I & II at 50 and 24 ºC respectively. Yeast extract and peptone were the best nitrogen sources for highest production of inulinases I & II at five and seven days of incubation respectively. The two inulinases I & II were purified to homogeneity by gel-filtration and ion-exchange chromatography with 66.0 and 42.0 fold of purification respectively. The optimum temperatures of purified inulinases I & II were 75 and 50 ºC respectively. Inulinase I was more thermostable than the other one. The optimum pH for activity was found to be 4.5 and 5.5 for inulinases I & II respectively. A comparatively lower Michaelis-Menten constant (2.15 mg/ml and higher maximum initial velocity (115 µmol/min/mg of protein for inulinase I on inulin demonstrated the exoinulinase's greater affinity for inulin substrate. These findings are significant for its potential industrial application. The molecular mass of the inulinases I & II were estimated to be 72 & 78 kDa respectively by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

  9. Effects of fly-ash treatment of soil on yields and chemical composition of chicory

    Energy Technology Data Exchange (ETDEWEB)

    Scotti, I.A.; Lombi, E.; Carini, F.; Silva, S. [Universita Cattolica Sacro Cuore, Piacenza (Italy). Ist. di Chimica Agraria e Ambientale

    1996-09-01

    In pot trials, chicory (Cichorium intybus) was grown on two soils with and without two levels (3% and 10%) of fly ash. It was observed that the addition of 3% fly ash gives rise to a significant increase in the yield and in Mg content, compared to a traditional dressing; all the other elements taken into account (Ca, S, Zn, Mn, Co, Pb, Ni, Cu and B) show positive or negative changes which, in most cases are not significant. With the addition of 10% fly ash the yield decreased, while the concentrations of all the elements, except Mn whose concentration in an acid soil undergoes a reduction, in general show significant increases.

  10. Selection and validation of reference genes for quantitative real-time PCR analysis of gene expression in Cichorium intybus

    OpenAIRE

    Delporte, Marianne; Legrand, Guillaume; Hilbert, Jean-Louis; Gagneul, David

    2015-01-01

    Plant polyphenols represent a huge reservoir of bioactive compounds. Industrial chicory, an important crop from northwestern Europe, accumulates an original combination of such compounds, i.e., chlorogenic, isochlorogenic, caftaric, and chicoric acids arising from the phenylpropanoid pathway. For a complete understanding of these biochemical pathways, analyses of gene expression using quantitative real-time PCR (qRT-PCR) should be considered. Because cell cultures are a model of choice for sp...

  11. Effects of the Mixture of Cichorium intybus L. and Cinnamomum zeylanicum on Hepatic Enzymes Activity and Biochemical Parameters in Patients with Nonalcoholic Fatty Liver Disease

    OpenAIRE

    Zeynab Sheybani Asl; Ali Akbar Malekirad; Mohammad Abdollahi; Alireza Bakhshipour; Hajar Akbari Dastjerdi; Sara Mostafalou; Razieh Yousef Poor

    2014-01-01

    The prevalence of nonalcoholic fatty liver disease (NAFLD) as a metabolic disorder affecting the liver function is rapidly increasing and there is a need to develop new and more efficient treatment. This study was designed to evaluate the protective effect of Cichorium intybus L. and Cinnamon mixture infusion (2.5 and 0.5 g/100mL and twice/day) on patients with NAFLD. This before-after clinical trial study was performed on 25 patients with NAFLD. They were administered the mixture of extract ...

  12. Introduction Test of Eurepean Chicory (Cichorium intybus L.)%欧洲菊苣引种试验初报

    Institute of Scientific and Technical Information of China (English)

    陆翠芳

    2008-01-01

    通过对优质牧草欧洲菊苣"甜馨"和"普娜"引种试验,掌握其植物学特征及增产效果,熟悉栽培技术.菊苣在众多优质牧草中以适口性好、适应性与抗病力强、易于栽培、产草量高、一次种植可多年利用等优点深受广大养殖户青睬.

  13. Reduced sexual compatibility between cultivated and wild chicory and their F1 hybrids

    DEFF Research Database (Denmark)

    Hauser, T.P.; Bagger Jørgensen, Rikke; Toneatto, F.

    2012-01-01

    Crops were domesticated from wild plants not too long ago and have subsequently diverged from the wild ones, especially in traits used by humans. Whether divergence between the cultigen and wild forms has also lead to reduced reproductive compatibility is unknown for many species. Chicory...... (Cichorium intybus L.) has been bred as a crop at least since Roman times. To test if this has led to a loss in reproductive compatibility with wild chicory, we planted cultivar, wild, and F1 hybrid plants into two field plots, and let them pollinate freely. On 2 days, in the beginning and middle of the...... marked, and when seeds were ripe we determined whether cultivar, wild or hybrid plants had pollinated the seeds, using AFLP markers. Cultivar plants fathered much fewer seeds than expected, both on wild and hybrid plants, suggesting that some degree of incompatibility has evolved between cultivar and...

  14. The Induction By Laser Light Of The In Vitro Flowering Of Cichorium intybus L. (Long day plant)

    Science.gov (United States)

    Paulet, Pierre; Badila, Philippe

    1980-05-01

    Flower inflorescences can be induced directly on root explants from the late variety of chicory cultivated in vitro. In vitro explants require cold treatment and long days to produce flowers. Initial studies on the level of endogenous phenolics and cytokinins in root explants during chilling treatment showed important changes and similar studies were desirable on the effect of long day treatments. However, during long days it is quite difficult to know if variations in these compounds depend on the trophic effect of light, i.e., photosynthesis or on the photoperiodic effect, i.e., tautomeric change in the structure of phytochrome. To separate photoperiodic effects from photosynthetic effects, explants were maintained in short days (10 hours light) and given photoperiodic induction by very brief irradiation with a dye laser producing 660 nn light of 150 mw/cm2. Controls in short days do not flower but explants irradiated for only 0.5 minute by the laser produced flowers. Significant changes in dicaffeylquinic acids (isochlorogenic acid) were obtained after only 7 seconds irradiation which are also correlated with flower induction. The results suggest that laser irradiation may prove important in agricultural practice.

  15. Dairy cows increase ingestive mastication and reduce ruminative chewing when grazing chicory and plantain.

    Science.gov (United States)

    Gregorini, P; Minnee, E M K; Griffiths, W; Lee, J M

    2013-01-01

    Although the nutritive value of chicory (Cichorium intybus L.) and plantain (Plantago lanceolata L.) has been thoroughly studied, little is known about the grazing behavior of cattle feeding on chicory and plantain swards. The objective of the present study was to assess and describe the grazing behavior of dairy cows as affected by dietary proportions of chicory and plantain fed as monocultures for part of the day. Ninety Holstein-Friesian cows (489±42 kg of body weight; 4.1±0.3 body condition score, and 216±15 d in milk) were randomly assigned to 15 groups (6 cows per group) and grazed according to 7 treatments: control (CTL, 3 groups), perennial ryegrass (Lolium perenne L.) dominant sward (24-h pasture strip); 3 chicory treatments comprising 20, 40, and 60% of the diet, strip-grazing a monoculture of chicory to a fixed postgrazing residual before strip-grazing a perennial ryegrass dominant sward (2 groups of cows per treatment); and 3 plantain treatments comprising 20, 40, and 60% of the diet, strip-grazing a monoculture of plantain to a fixed postgrazing residual before strip-grazing a perennial ryegrass dominant sward (2 groups of cows per treatment). Four focal animals per group were equipped with 3-dimensional motion sensors, which provided the number of steps taken at each minute of the day. These cows were also fitted with automatic jaw-movement recorders that identified bites, mastication during ingestion, chewing during rumination, and determined grazing, rumination and idling times and bouts. Daily grazing time and bouts were not affected by treatments but rumination time differed and was reduced by up to 90 min when cows were allocated to chicory and plantain as 60% of their diet. Ruminative chewing was reduced in cows grazing chicory and plantain by up to 20% in cows allocated to the 60% treatments. Compared with perennial ryegrass, as the dietary proportion of chicory and plantain increased, cows spent more time idling and less time ruminating

  16. CHARACTERISATION OF INULIN FROM CHICORY AND SALSIFY CULTIVATED IN PORTUGAL

    Directory of Open Access Journals (Sweden)

    M. L. BEIRÃO-DA-COSTA

    2009-03-01

    Full Text Available

    Inulin and fructooligosaccharides (FOS are important ingredients used in the food industry because of their diverse nutritional and functional properties. Among the sources of these compounds, chicory (Cichorium intybus L. is one of the most important, due to its high content of inulin, which shows a high F/G ratio. As in several European countries, chicory is grown in Portugal where is used traditionally as a coffee substitute. However, studies have been carried out concerning the evaluation of the characteristics of the carbohydrate fraction of Portuguese chicory, to investigate its industrial potential as a raw material for the production of inulin, FOS and fructose. Salsify (Tragopogon porrifolius L., another plant generally regarded as an inulin source, is also cultivated in the same regions of our country where it is used as a cooked vegetable. Carbohydrate constituents of chicory and salsify roots, mainly inulin and FOS, were submitted to a physical-chemical analysis by HPLC and DSC (Differential Scanning Calorimetry. The viscosities of the gels produced from both inulins were also evaluated. From HPLC results it was apparent that great differences exist between the inulin sources, mainly in degree of polymerisation which was higher in chicory. Endotherms showed that both peak temperature and enthalpy values of the transition were different, the latter being much higher for salsify. KEYWORDS: Physical-chemical analysis of inulin; chicory; salsify.

  17. 不同土壤不同种植方式普那菊苣的产量分析%Study on planting model of Cichorium intybus cv.Puna sown in various soil

    Institute of Scientific and Technical Information of China (English)

    韩永芬; 孟军江; 左相兵; 舒健虹; 彭佳

    2009-01-01

    Seeds of Cichorium intybus cv.Puna was sown in sandy soil, clay soil and loam soil with hole seeding, drill seeding, broadcast seeding and transplantation with seedlings, and then recorded the fresh weight and growth rates. The results showed that fresh weight were considerably high in all treatments. However, fresh weights were different within treatments, in the same soil, drill seeding>hole seeding>transplantation;and in the same planting ways, loam soil >sandy soil>clay soil. Therefore, the plant adopted the region but line seeding and hole seeding should be the most suitable planting model.%在砂土、粘土、壤土3种不同的土壤上以穴播、条播、撒播和育苗移栽4种种植方式种植黔引普那菊苣Cichorium intybus cv.Puna,测定其鲜草产量和再生速度.结果表明:黔引普那菊苣在各种不同质地土壤上采用各种种植方式均有较高的产量.在同质地土壤上,鲜草产量以条播为最高,穴播次之,育苗移栽产量最低.同一播种方式以在壤土上的产量为最高,砂土次之,粘土最低.再生速度在不同处理之间差异均不显著.黔引普那菊苣适宜在各种土壤上种植,但以在土壤肥沃的壤土上种植产量较高,条播、穴播为推广的种植方式.

  18. Production Test of Chicory Cultivar Qianyu 1%黔育1号菊苣生产试验

    Institute of Scientific and Technical Information of China (English)

    张伟; 苏生; 韩永芬; 孟军江; 吴有松; 周秀丽

    2014-01-01

    Selecting four representative counties or cities ( Dushan County,Daozhen County,Bijie City and Songtao County ) of Guizhou Province as experimental sites, the field production test on chicory cultivar Qianyu 1 was made with Cichorium intybus cv. Puna as control. The fresh grass yield and hay yield of chicory cultivar Qianyu 1 planted in different areas were determined and the production level of chicory cultivar Qianyu 1 was discussed, so as to provide scientific basis for its large-scale production. The result showed that the yield of chicory cultivar Qianyu 1 in the production test in four experimental sites was all higher, and its average yield of fresh grass in Dushan County, Daozhen County, Bijie City and Songtao County was all more than 95 001.6 kg/hm 2. The average yield of fresh grass of Cichorium intybus cv. Puna was 83 732.0 kg/hm2. And the average output of chicory cultivar Qianyu 1 increased by 13.4%than that of Cichorium intybus cv. Puna.%选择贵州省独山县、道真县、毕节市、松桃县4个具有代表性的县(市)为试点,以普那菊苣为对照品种,对黔育1号菊苣进行了大田生产试验。旨在通过对在不同地区种植的黔育1号菊苣鲜、干草产量的测定,来明确黔育1号菊苣的生产力水平,从而为其将来大面积用于生产提供科学依据。结果表明,4个试点生产试验中,黔育1号菊苣的产量均较高,其在独山县、道真县、毕节市、松桃县的平均鲜草产量为95001.6 kg/hm2以上,普那菊苣的平均鲜草产量为83732.0 kg/hm2,黔育1号菊苣比对照品种普那菊苣平均增产13.4%。

  19. Intra- and interspecific chromosome polymorphisms in cultivated Cichorium L. species (Asteraceae

    Directory of Open Access Journals (Sweden)

    Ebenézer C.S. Bernardes

    2013-01-01

    Full Text Available Endive (Cichorium endivia L. and chicory (C. intybus L. both have 2n = 18, but until now, there has been no detailed karyomorphological characterization. The present work evaluated five accessions of each species using FISH with rDNA probes and fluorochrome staining with CMA and DAPI. Both species presented distinct banding patterns after fluorochrome staining: while endive had proximal CMA++/DAPI- bands in the short arms of pairs 1, 2 and 3, chicory had proximal CMA-positive bands in chromosomes 1 and 3 and interstitial in the short arm of chromosome 8. Among endive accessions, FISH procedures revealed conserved position and number of 5S and 45S rDNA sites (two and three pairs, respectively, associated with the CMA-positive bands. Notwithstanding, polymorphisms were detected within chicory accessions regarding the number and the distribution of rDNA sites in relation to the most frequent karyotype (two pairs with 45S and one with 5S rDNA. The karyological markers developed allowed karyotypic differentiation between both species, uncovering peculiarities in the number and position of rDNA sites, which suggest chromosome rearrangements, such as translocations in chicory cultivars. The interspecific and intraspecific polymorphisms observed emphasize the potential of karyomorphological evaluations, helping our understanding of the relationships and evolution of the group.

  20. Intra- and interspecific chromosome polymorphisms in cultivated Cichorium L. species (Asteraceae).

    Science.gov (United States)

    Bernardes, Ebenézer C S; Benko-Iseppon, Ana M; Vasconcelos, Santelmo; Carvalho, Reginaldo; Brasileiro-Vidal, Ana C

    2013-09-01

    Endive (Cichorium endivia L.) and chicory (C. intybus L.) both have 2n = 18, but until now, there has been no detailed karyomorphological characterization. The present work evaluated five accessions of each species using FISH with rDNA probes and fluorochrome staining with CMA and DAPI. Both species presented distinct banding patterns after fluorochrome staining: while endive had proximal CMA(++)/DAPI(-) bands in the short arms of pairs 1, 2 and 3, chicory had proximal CMA-positive bands in chromosomes 1 and 3 and interstitial in the short arm of chromosome 8. Among endive accessions, FISH procedures revealed conserved position and number of 5S and 45S rDNA sites (two and three pairs, respectively), associated with the CMA-positive bands. Notwithstanding, polymorphisms were detected within chicory accessions regarding the number and the distribution of rDNA sites in relation to the most frequent karyotype (two pairs with 45S and one with 5S rDNA). The karyological markers developed allowed karyotypic differentiation between both species, uncovering peculiarities in the number and position of rDNA sites, which suggest chromosome rearrangements, such as translocations in chicory cultivars. The interspecific and intraspecific polymorphisms observed emphasize the potential of karyomorphological evaluations, helping our understanding of the relationships and evolution of the group. PMID:24130443

  1. A review on pharmic effect of chicory research and development%菊苣的药理药效研究及开发前景

    Institute of Scientific and Technical Information of China (English)

    王俭珍; 崔健

    2009-01-01

    Chicory (Cichorium intybus L) is a bushy perennial herb with blue, lavender, or occasionally white flowers. Itgrows as a wild plant on roadsides in its native Europe, and in North America, where it has become naturalized. Common chicory is also known as blue sailors, succory, and coffeeweed. Chicory contains saccharides, organic acid, alkaloid, triterpenes, sesquiterpenes,coumarins, and so on. It has a function of lowering the blood glucose and lipid, decreasing uric acid, and hepatoprotection. Therefore,it is evacuant and appetitive with better cardiovascular effect. Furthermore, it can be sorbefacient calcium, enhancing immunity via antiallergic, antibacterial and antivirus. So, with research and development on the peculiar physiology function of chicory, it must have a bright prospect on discovering salubrious beverage, functional food and remedy with chicory at present and near future.%菊苣Cichorium intybus L.是菊科菊苣属多年宿根植物.菊苣的主要成分有糖类、有机酸类、生物碱类、三萜类、倍半萜类和香豆素等;其药效研究主要有降血糖、降血脂、降尿酸作用、保肝作用以及对消化系统和心血管系统的影响作用,并在促进对钙的吸收利用,提高机体免疫力,抗过敏、抗菌、抗病原微生物方面有一定的作用;目前对药效利用主要用于保健饮品、功能性食品和特效药品进行开发,未来在特效药品开发方面极具开发价值和利用前景.

  2. Effect of chicory seed extract on glucose tolerance test (GTT and metabolic profile in early and late stage diabetic rats

    Directory of Open Access Journals (Sweden)

    Ali Ahadi

    2012-10-01

    Full Text Available Background and purpose of the study The goal was to evaluate and compare the effects of aqueous extract of the seeds of chicory, Cichorium intybus L., on glucose tolerance test (GTT and blood biochemical indices of experimentally-induced hyperglycemic rats.MethodsLate stage and early stage of Type 2 diabetes mellitus (T2DM were induced in rats by streptozotocin (STZ and a combination of STZ and niacinamide (NIA/STZ, respectively. Within each group, one subgroup received daily i. p. injections of chicory extract (125 mg/kg body weight, for 28 days. Body weight and fasting blood sugar (FBS were measured weekly. Blood was analyzed for glycosylated hemoglobin (HbA1c and sera for alanine aminotransferase (ALT, aspartate aminotransferase (AST, nitric oxide (NO, triacylglycerol (TG, total cholesterol (TC, total protein, and insulin on days 10 and 28 after treatment. Intraperitoneal glucose tolerance test (IPGTT along with insulin determination was performed on a different set of rats in which the chicory-treated groups received the extract for 10 days.ResultsDuring 4 weeks of treatment, chicory prevented body-weight loss and decreased FBS. ALT activities and levels of TG, TC and HbA1c decreased, and concentration of NO increased in the chicory treated groups (p < 0.05. Unlike late-stage diabetes, fasting serum insulin concentrations were higher and GTT pattern approximated to normal in chicory-treated earlystage diabetic rats.ConclusionsChicory appeared to have short-term (about 2 hours, as far as GTT is concerned and longterm (28 days, in this study effects on diabetes. Chicory may be useful as a natural dietary supplement for slowing down the pace of diabetes progress, and delaying the development of its complications.

  3. Effect of chicory seed extract on glucose tolerance test (GTT and metabolic profile in early and late stage diabetic rats

    Directory of Open Access Journals (Sweden)

    Ghamarian Abdolreza

    2012-10-01

    Full Text Available Abstract Background and purpose of the study The goal was to evaluate and compare the effects of aqueous extract of the seeds of chicory, Cichorium intybus L., on glucose tolerance test (GTT and blood biochemical indices of experimentally-induced hyperglycemic rats. Methods Late stage and early stage of Type 2 diabetes mellitus (T2DM were induced in rats by streptozotocin (STZ and a combination of STZ and niacinamide (NIA/STZ, respectively. Within each group, one subgroup received daily i. p. injections of chicory extract (125 mg/kg body weight, for 28 days. Body weight and fasting blood sugar (FBS were measured weekly. Blood was analyzed for glycosylated hemoglobin (HbA1c and sera for alanine aminotransferase (ALT, aspartate aminotransferase (AST, nitric oxide (NO, triacylglycerol (TG, total cholesterol (TC, total protein, and insulin on days 10 and 28 after treatment. Intraperitoneal glucose tolerance test (IPGTT along with insulin determination was performed on a different set of rats in which the chicory-treated groups received the extract for 10 days. Results During 4 weeks of treatment, chicory prevented body-weight loss and decreased FBS. ALT activities and levels of TG, TC and HbA1c decreased, and concentration of NO increased in the chicory treated groups (p Conclusions Chicory appeared to have short-term (about 2 hours, as far as GTT is concerned and long-term (28 days, in this study effects on diabetes. Chicory may be useful as a natural dietary supplement for slowing down the pace of diabetes progress, and delaying the development of its complications.

  4. Biotransformation of (±)-4,8-dimethylcyclodeca-3(E),7(E)-dien-1β-ol and (+)-Hedycaryol by Cichorium intybus.

    OpenAIRE

    Piet, Dennis P.; Adriaan J. Minnaard; Heyden, Karel A. van der; Franssen, Maurice C. R.; Wijnberg, Joannes B. P. A.; Groot, Aede de

    1995-01-01

    The biotransformation of the synthetic (E,E)-1,5-cyclodecadienol 5 and (+)-hedycaryol (11) by a root suspension of fresh chicory has been investigated. Incubation of 5 with a root suspension gave a 2 : 1 mixture of the epimeric eudesmanediols 7a and 7b whereas 11 was selectively converted into cryptomeridiol (12). An explanation for the obtained results is proposed.

  5. Performance and dietary preferences of white-tailed deer grazing chicory, birdsfoot trefoil or alfalfa in north central Alberta.

    Science.gov (United States)

    Chapman, G A; Bork, E W; Donkor, N T; Hudson, R J

    2009-12-01

    Little information exists on the performance of deer on alternative forage species in northern temperate environments during summer and fall, the period of inherent maximum growth in deer. In performance and choice experiments, we compared live weight gain (g/kg(0.75)/day), absolute [kg/ha dry matter (DM)] and relative (% DM) herbage utilization, relative preference index (RPI) as well as plant community visitation of white-tailed deer grazing alfalfa (Medicago sativa), birdsfoot trefoil (Lotus corniculatus) or chicory (Cichorium intybus) in north central Alberta, Canada. Herbage phytomass and quality was also measured on the grazed pastures. Alfalfa had higher dry matter yields and crude protein concentrations than chicory and trefoil. Chicory had lower neutral detergent fiber concentrations than the other forages. Tannin concentrations were greatest in birds foot trefoil (nearly 55 g/kg DM), well above those in the other forages ( 0.05) among the three forage species. In contrast, relative herbage utilization was greater from birds foot trefoil (52% DM) than chicory (40% DM) or alfalfa (25% DM). These results suggest that the use of alfalfa with other alternative forages may prove beneficial to deer production, rather than using alfalfa pasture alone. PMID:19138349

  6. Effect of IAA on growth, organogenesis and RNA metabolism during the development of Cichorium intybus root explants cultured „in vitro"

    OpenAIRE

    E. Gwóźdź

    2015-01-01

    The effects of 3-indolylacetic acid (IAA) on growth, organogenesis, RNA content, RNase activity and MAK elution profiles during the development of chicory root explants cultured in vitro were investigated. It was found that the intensive callus growth in the presence of IAA was accompanied by an increase in the RNA content, with simultaneous decrease of RNase activity. Fractionation of RNA by MAK column chromatography showed that the high content of RNA in the callus under the influence of IA...

  7. The effect of 3-indolylacetic acid on the accumulation of starch in the root tissue of Cichorium intybus L. cultured in vitro

    Directory of Open Access Journals (Sweden)

    U. Sobczyk

    2015-05-01

    Full Text Available The relation between IAA-induced formation of amyloplasts in callus cells of chicory root and the influence of IAA on sugar uptake from the medium was investigated. Experiments with 14C-sucrose showed that IAA increased the uptake of sucrose from the medium. The amyloplast-like structures were also observed in callus grown on medium without IAA, but containing high concentration of sucrose (9%. The possibility of IAA influence on the formation of amyloplasts by increasing the permeability of cells for sugar is discussed.

  8. Hindiba (Cichorium intybus L. Bitkisinden Myrosinaz Enziminin Saflaştırılması, Karakterize Edilmesi ve Kozmetik Alanında Kullanılabilirliğinin İncelenmesi

    Directory of Open Access Journals (Sweden)

    Nazan DEMİR

    2014-07-01

    Full Text Available Myrosinaz glukosinolatların hidrolizinden sorumlu bir enzimdir (E.C: 3.2.3.2 β-thioglucosidase, β-thioglucoside glucohydrolase olarak da bilinir. Kanserle mücadele için umut verici bileşikler olarak kabul edilen sinigrin, glukoraphanin (GRA ve glukoraphenin (GRE gibi ara yıkım ürünlerinden dolayı bazı alifatik glukosinolatlar oldukça ilgi çekmiş ve myrosinaz enziminin bu tümör küçültücü etkisi son yıllarda önem kazanmıştır. Bu çalışmada, myrosinaz amonyum sülfat çöktürmesi, Q-sepharose, Konkanavalin A sepharose afinite kromatografisi ile Hindiba'dan (Cichorium intybus'un L. homojen olarak saflaştırıldı. Saflaştırılan protein SDS- poliakrilamid jel elektroforezinde 35 kDa ağırlığında tek bir bant olarak gözlenmiştir. Enzim pH 8,0'de ve 70 °C' de maksimum aktivite göstermiştir. Saflaştırılmış enzim 1 yıldan fazla süreyle 4° C de sabit kalmıştır. Substrat olarak sinigrin kullanılarak, saflaştırılmış enzim için KM ve Vmax değerleri sırasıyla 0,02 ve 0,074 μmol glukoz. min–1.mg–1 olarak belirlenmiştir. Enzim askorbik asit (AA, EDTA, SDS ve β-merkaptoethanol tarafından güçlü bir şekilde aktive edilmiştir. Buna ek olarak, bazı iyonların myrosinaz enzim aktivitesi üzerine 10, 1 ve 0,1 mM'lık konsantrasyonlardaki etkileri (Cu2+, Ni2+, Ca2+ incelenmiş ve enzim aktivitesini önemli ölçüde etkiledikleri belirlenmiştir. Hindiba (Cichorium intybus'un L.'dan elde edilen myrosinaz enziminin kozmetikte kullanılabilirliliği araştırılmıştır. Çalışmada saf myrosinaz enzimi içeren krem oluşturulmuş ve karışım güneş lekesi ve benzeri cilt sorunları olan 10 gönüllü denek üzerinde 20 gün süreyle uygulanmıştır. Belirlenen gün sonunda deneklerde mevcut olan lekelerin şiddetinde kısmi azalmalar gözlenmiştir.

  9. Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus) show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi

    DEFF Research Database (Denmark)

    Pena-Espinoza, Miguel Angel; Boas, Ulrik; Williams, Andrew;

    2015-01-01

    concentrations of SL-containing extracts in PBS (1% DMSO) were tested in replicates with 1% DMSO in PBS as negative controls. HPLC demonstrated similar concentrations of most SL in both extracts. However, Spadona-extract contained significantly higher concentrations of 11, 13-dihydro-8-deoxylactucin (P = 0...

  10. Construction and characterization of two BAC libraries representing a deep-coverage of the genome of chicory (Cichorium intybus L., Asteraceae)

    OpenAIRE

    Gonthier Lucy; Bellec Arnaud; Blassiau Christelle; Prat Elisa; Helmstetter Nicolas; Rambaud Caroline; Huss Brigitte; Hendriks Theo; Bergès Hélène; Quillet Marie-Christine

    2010-01-01

    Abstract Background The Asteraceae represents an important plant family with respect to the numbers of species present in the wild and used by man. Nonetheless, genomic resources for Asteraceae species are relatively underdeveloped, hampering within species genetic studies as well as comparative genomics studies at the family level. So far, six BAC libraries have been described for the main crops of the family, i.e. lettuce and sunflower. Here we present the characterization of BAC libraries ...

  11. Carcass parameters and meat quality in meat-goat kids finished on chicory, birdsfoot trefoil, or red clover pastures.

    Science.gov (United States)

    Turner, K E; Cassida, K A; Zerby, H N; Brown, M A

    2015-07-01

    This study was conducted during the 2009 and 2010 grazing seasons to assess carcass parameters and chevon (goat meat) quality when meat-goat kids (n=72) were finished on pastures of red clover (Trifolium pratense L.; RCL), birdsfoot trefoil (Lotus corniculatus L.; BFT), or chicory (Cichorium intybus L.; CHIC). Final live weight (P0.10) among treatments when adjusted for the covariate of carcass weight. Finishing meat-goat kids on RCL, BFT, or CHIC impacted concentrations of fatty acids (FAs) 18:1 trans-10, 18:1 cis-11, 18:2, 18:3 polyunsaturated fatty acids (PUFAs), omega-6, omega-3, and PUFA:saturated fatty acid ratio in longissimus lumborum samples. Finishing meat-goat kids on CHIC, RCL, or BFT pastures produced carcass weights acceptable for most ethnic markets in the USA. PMID:25817803

  12. Use of a Plackett-Burman experimental design to examine the impact of extraction parameters on yields and compositions of pectins extracted from chicory roots (Chicorium intybus L.).

    Science.gov (United States)

    Robert, Christelle; Devillers, Thierry; Wathelet, Bernard; Van Herck, Jean-Claude; Paquot, Michel

    2006-09-20

    Chicory root pectin was isolated by acid extraction followed by alcohol precipitation. Because the extraction conditions have important effects on the features of pectins, an experimental design was used to study the influence of 17 different extraction parameters on yield and composition of pectin: pH, temperature, time of extraction, solid/liquid ratio, and different pretreatments of the pulps before extraction. Twenty extractions were conducted and examined for their significance on yield and sugar content using the Plackett-Burman factorial design. The acid extraction of chicory roots resulted in an average yield of 11% containing 86% of sugars. It was found that extraction temperature, time, protease pretreatment, water purity, and water washing of pulps significantly affected yield and pectin composition with an increase of yield and purity of pectin in harsher extraction conditions. PMID:16968078

  13. Effect of IAA on growth, organogenesis and RNA metabolism during the development of Cichorium intybus root explants cultured „in vitro"

    Directory of Open Access Journals (Sweden)

    E. Gwóźdź

    2015-05-01

    Full Text Available The effects of 3-indolylacetic acid (IAA on growth, organogenesis, RNA content, RNase activity and MAK elution profiles during the development of chicory root explants cultured in vitro were investigated. It was found that the intensive callus growth in the presence of IAA was accompanied by an increase in the RNA content, with simultaneous decrease of RNase activity. Fractionation of RNA by MAK column chromatography showed that the high content of RNA in the callus under the influence of IAA was due to an increased accumulation of the ribosomal fraction of RNA mainly. Experiments with actinomycin D demonstrated that this antibiotic abolished both the auxin-induced callus growth and the inhibitory effect of IAA on bud formation. No significant inhibition of spontaneous bud formation under the influence of actinomycin D was observed. The possible relationship between the IAA-affected morphogenetic processes and RNA metabolism is discussed.

  14. 电阻抗分析在菊苣根冻害检测中的应用研究%Application of Electrical Impedance Analysis to Assessment of Freezing Injury in Chicory Roots

    Institute of Scientific and Technical Information of China (English)

    陈发河; MauiceDeProft

    2002-01-01

    以菊苣(Cichorium intybus L var foliosum cv.)根为试材,分析了低温下电阻抗和钾离子渗漏速率的变化及其与细胞膜透性变化的定量关系.结果表明:随着低温贮藏时间的延长和冻害的发展,菊苣根细胞膜透性增大,钾离子渗漏速率上升,组织的电阻抗值下降.3种温度下(0℃,-1℃,-3℃)贮藏的菊苣根其电阻抗值的变化均与钾离子渗漏速率呈显著的负相关关系.电阻抗对频率作图显示,在低频率范围(20~100 Hz)可得到最大而且较稳定的电阻抗值.因此,电阻抗分析方法可以用来检测和评价植物的冻害.%Chicory (Cichorium intybus L var foliosum cv.) root was used to investigate the change in electrical impedance and potassium ion leakage during cold storage, and some quantitative relations between the electrical impedance and cell membrane permeability. It is showed that the cell membrane permeability, and the rate of potassium ion leakage increase, while the electrical impedance decreases with the development of freezing injury in chicory root. The change in electrical impedance in chicory roots stored at 0℃, -1℃, -3℃ was remarkable negatively correlated with the potassium ion leakage rate. At low frequency there was relatively little change and the highest value in impedance (20~100 Hz). Therefore, it is feasible that the electrical impedance analysis can be used to estimate the freezing injury of plant.

  15. Biochemical changes involved in browning of gamma-irradiated cut witloof chicory

    International Nuclear Information System (INIS)

    The increasing browning phenomenon of cut witloof chicory (Cichorium intybus) irradiated with a dose of 3 kGy and stored five days at 10 °C was investigated in relation to total phenolic concentration, phenylalanine ammonia-lyase activity, total and reduced glutathione content, polyphenol oxidase and peroxidase activities. Phenylalanine ammonia-lyase activity underwent a strong activation with a maximum on the second day of storage, and total phenolic compounds tended to accumulate from the third day. Glutathione content was monitored as indicator of antioxidant capacities. Total glutathione decreased by 25% after irradiation and was restored on the third day of storage, while the 50% decrease of reduced glutathione was maintained along the five days. Conversely, a 30% inhibition of polyphenol oxidase and peroxidase activities occured after irradiation. We concluded first that irradiation-induced browning of cut chicory may be due to both an increase of phenolic metabolism and a reduction in antioxidant capacities, and second that increased membrane permeability may allow substrate-enzyme contact

  16. Divergences in hormonal and enzymatic antioxidant responses of two Chicory ecotypes to salt stress.

    Science.gov (United States)

    Ghanaatiyan, Kimiya; Sadeghi, Hossein

    2015-06-15

    To evaluate the effect of salt stress on seed germination, early growth, antioxidant enzymes activity and ABA content of chicory ecotypes (Cichorium intybus) a factorial experiment was conducted at College of Agriculture, Shiraz University in 2014 based on completely randomized design with four replications. The treatments comprised five salinity levels (tapwater, 3, 6, 9, 12 dS m(-1)) of sodium chloride on Shirazi-black and white chicory ecotypes. The results showed that germination characteristics and primary seedling growth were decreased in both ecotypes with increasing in salinity severity. The effects of salinity on radicle and plumule length as well as seedling weight were the same as its effects on seed germination. The effect of salt stress on antioxidant enzymes activity (especially catalase) and ABA content were significant which they were enhanced with increasing salinity level; Black ecotype performs better than the white one under high salinity, as indicated by a lower decreasing in germination characteristics and primary growth and higher antioxidant enzymes activity as well as ABA content. These facts should be taken into consideration in the economic cultivation of this valuable horticultural and medicinal plant and this data would be useful for the crop breeding projects. PMID:26075934

  17. Effects of chicory/perennial ryegrass swards compared with perennial ryegrass swards on the performance and carcass quality of grazing beef steers.

    Directory of Open Access Journals (Sweden)

    Christina L Marley

    Full Text Available An experiment investigated whether the inclusion of chicory (Cichorium intybus in swards grazed by beef steers altered their performance, carcass characteristics or parasitism when compared to steers grazing perennial ryegrass (Lolium perenne. Triplicate 2-ha plots were established with a chicory/ryegrass mix or ryegrass control. Forty-eight Belgian Blue-cross steers were used in the first grazing season and a core group (n = 36 were retained for finishing in the second grazing season. The experiment comprised of a standardisation and measurement period. During standardisation, steers grazed a ryegrass/white clover pasture as one group. Animals were allocated to treatment on the basis of liveweight, body condition and faecal egg counts (FEC determined 7 days prior to the measurement period. The measurement period ran from 25 May until 28 September 2010 and 12 April until 11 October 2011 in the first and second grazing year. Steers were weighed every 14 days at pasture or 28 days during housing. In the first grazing year, faecal samples were collected for FEC and parasite cultures. At the end of the first grazing year, individual blood samples were taken to determine O. ostertagi antibody and plasma pepsinogen levels. During winter, animals were housed as one group and fed silage. In the second grazing year, steers were slaughtered when deemed to reach fat class 3. Data on steer performance showed no differences in daily live-weight gain which averaged 1.04 kg/day. The conformation, fat grade and killing out proportion of beef steers grazing chicory/ryegrass or ryegrass were not found to differ. No differences in FEC, O. ostertagi antibody or plasma pepsinogen levels of beef steers grazing either chicory/ryegrass or ryegrass were observed. Overall, there were no detrimental effects of including chicory in swards grazed by beef cattle on their performance, carcass characteristics or helminth parasitism, when compared with steers grazing ryegrass.

  18. Effects of chicory/perennial ryegrass swards compared with perennial ryegrass swards on the performance and carcass quality of grazing beef steers.

    Science.gov (United States)

    Marley, Christina L; Fychan, Rhun; Davies, John W; Scollan, Nigel D; Richardson, R Ian; Theobald, Vince J; Genever, Elizabeth; Forbes, Andy B; Sanderson, Ruth

    2014-01-01

    An experiment investigated whether the inclusion of chicory (Cichorium intybus) in swards grazed by beef steers altered their performance, carcass characteristics or parasitism when compared to steers grazing perennial ryegrass (Lolium perenne). Triplicate 2-ha plots were established with a chicory/ryegrass mix or ryegrass control. Forty-eight Belgian Blue-cross steers were used in the first grazing season and a core group (n = 36) were retained for finishing in the second grazing season. The experiment comprised of a standardisation and measurement period. During standardisation, steers grazed a ryegrass/white clover pasture as one group. Animals were allocated to treatment on the basis of liveweight, body condition and faecal egg counts (FEC) determined 7 days prior to the measurement period. The measurement period ran from 25 May until 28 September 2010 and 12 April until 11 October 2011 in the first and second grazing year. Steers were weighed every 14 days at pasture or 28 days during housing. In the first grazing year, faecal samples were collected for FEC and parasite cultures. At the end of the first grazing year, individual blood samples were taken to determine O. ostertagi antibody and plasma pepsinogen levels. During winter, animals were housed as one group and fed silage. In the second grazing year, steers were slaughtered when deemed to reach fat class 3. Data on steer performance showed no differences in daily live-weight gain which averaged 1.04 kg/day. The conformation, fat grade and killing out proportion of beef steers grazing chicory/ryegrass or ryegrass were not found to differ. No differences in FEC, O. ostertagi antibody or plasma pepsinogen levels of beef steers grazing either chicory/ryegrass or ryegrass were observed. Overall, there were no detrimental effects of including chicory in swards grazed by beef cattle on their performance, carcass characteristics or helminth parasitism, when compared with steers grazing ryegrass. PMID:24489708

  19. Increase in the Synthesis of Polyfructan in the Cultures of Chicory “Hairy Roots” with Plant Natural Growth Regulators

    Directory of Open Access Journals (Sweden)

    Victoria А. Tsygankova, PhD¹

    2013-06-01

    Full Text Available Experiments were conducted to study the benefit of using the new plant growth regulators (PGRs Ivin, Emistim, Biolan and Charkor in nutrient medium ½ MS for intensification of biomass growth and the increased synthesis of polyfructan (PF in the cultures of chicory “hairy roots” (Cichorium intybus L., obtained by Аgrobacterium rhizogenes-mediated transformation,. The best indexes of increased specific quantities of PF are observed after using Biolan at a concentration of 5.0 μL/L (up to 130 mg/g dry mass of roots and Emistim at a concentration 2.5 µL/L (up to 220 mg/g dry mass of roots. The greatest stimulation of root growth activity was expressed on using the growth regulators Emistim, Ivin and Charkor, in concentrations between 2.5 and 10.0 µL/L, considerably raising the total quantity of PF: compared with the control the use of regulator Emistim showed a rise of up to 35 times, regulator Ivin showed an increase of up to 28 times and regulator Charkor showed an increase up to 7.0-7.5 times. The results thus obtained definitely prove the benefit of applying these regulators to increase the biomass growth and PF synthesis in the culture of chicory «hairy roots».

  20. A root chicory MADS box sequence and the Arabidopsis flowering repressor FLC share common features that suggest conserved function in vernalization and de-vernalization responses.

    Science.gov (United States)

    Périlleux, Claire; Pieltain, Alexandra; Jacquemin, Guillaume; Bouché, Frédéric; Detry, Nathalie; D'Aloia, Maria; Thiry, Laura; Aljochim, Pierre; Delansnay, Martin; Mathieu, Anne-Sophie; Lutts, Stanley; Tocquin, Pierre

    2013-08-01

    Root chicory (Cichorium intybus var. sativum) is a biennial crop, but is harvested to obtain root inulin at the end of the first growing season before flowering. However, cold temperatures may vernalize seeds or plantlets, leading to incidental early flowering, and hence understanding the molecular basis of vernalization is important. A MADS box sequence was isolated by RT-PCR and named FLC-LIKE1 (CiFL1) because of its phylogenetic positioning within the same clade as the floral repressor Arabidopsis FLOWERING LOCUS C (AtFLC). Moreover, over-expression of CiFL1 in Arabidopsis caused late flowering and prevented up-regulation of the AtFLC target FLOWERING LOCUS T by photoperiod, suggesting functional conservation between root chicory and Arabidopsis. Like AtFLC in Arabidopsis, CiFL1 was repressed during vernalization of seeds or plantlets of chicory, but repression of CiFL1 was unstable when the post-vernalization temperature was favorable to flowering and when it de-vernalized the plants. This instability of CiFL1 repression may be linked to the bienniality of root chicory compared with the annual lifecycle of Arabidopsis. However, re-activation of AtFLC was also observed in Arabidopsis when a high temperature treatment was used straight after seed vernalization, eliminating the promotive effect of cold on flowering. Cold-induced down-regulation of a MADS box floral repressor and its re-activation by high temperature thus appear to be conserved features of the vernalization and de-vernalization responses in distant species. PMID:23581257

  1. 低温对葡萄孢菌( Botrytis cinerea )菌丝生长和孢子萌发以及对贮藏菊苣侵染力的影响%EFFECTS OF LOW TEMPERATURE ON MYCELIAL GROWTH AND SPORE GERMINATION OF Botrytis cinerea IN VITRO AND ON ITS INFECTIVITY TO STORED CHICORY

    Institute of Scientific and Technical Information of China (English)

    田世平

    2001-01-01

    本文主要研究葡萄孢菌(Botrytis cinerea)在20、10、4、2、0、—2和—4℃下菌丝生长和孢子萌发的情况,以及在同样温度下对菊苣(Cichorium intybus L.)致病力的影响。尽管在PDA培养基上0℃以下低温明显地抑制菌丝生长和推迟孢子的萌发时间,但葡萄孢菌仍能在—4℃下14 d后达到100%的萌发率,24周后菌落的生长直径为10 mm。用Botrytis cinerea孢子接种的菊苣在—2℃下6周以前的发病率较低,在—4℃下8周以前没有病害发生,但随着贮藏时间的延长,发病率逐渐上升,12周后腐烂率达到77%和71%,病害严重程度指数分别为37和31。菊苣贮藏在—2℃和—4℃下后期腐烂率的快速增加可能与此时菊苣产生冷害有关。%The effects of low temperature ranging from 20℃ to —4℃ on mycelial growth, the conidial germination of Botrytis cinerea in culture and its infectivity to stored chicory (Cichorium intybus L. ) were investigated. Although temperatures below 0℃ could inhibit the mycelial growth and delay the conidial germination, B. cinerea was able to grow and germinate on PDA and to infect chicory even at —4℃. Germination rate reached 100% after 14 days and mycelial diameter was 10 mm after 24 weeks at such temperature. No decay was found in chicory kept at —4℃ before 8 weeks and there was very low disease incidence in chicory stored at —2℃ in 6 weeks of storage. After 12 weeks in storage, disease incidence reached 77% and 71% with a disease severity score of 37 and 31, respectively, which may be related partly to freezing injury of chicory stored at such temperatures.

  2. Influence of different irrigation levels on the root water uptake and the physiology of root-chicory

    Science.gov (United States)

    Vandoorne, B.; Dekoninck, N.; Lutts, S.; Capelle, B.; Javaux, M.

    2009-04-01

    In the context of global warming and given recent heat waves observed in Western Europe, the relationship between the soil water status and the plant health has recently received more attention, especially for cash crops like chicory. In this study we particularly investigated the impact of soil water status on the chicory root water uptake and density and made a link with physiological and yield parameters. During five months, we imposed different irrigation levels to 10 plants of chicory (Cichorium intybus var. sativum) growing in greenhouses. Each seed, coming from an autogamous selection in this allogamous species, was sown in a column of 1.42m height and 0.4m diameter filled with yellow sand and irrigated from the bottom with Hoagland solution. On those 10 columns, we measured the distribution of soil moisture with TDR (8 columns) and ERT (2 columns) probes. Lateral windows also allowed us to follow the root growth. The column weights were also monitored in order to quantify the plant transpiration. During the experiment, several physiological indices were also followed like the gas exchange (CO2 and transpiration), the chlorophyll fluorescence, the stomatal conductance, the plastochron, and the Leaf Area Index (LAI). At the end of the experiment, the complete root length density and the water content profiles were measured. We had also a look to the osmotic potential, the pigments content and the isotopic discrimination of carbon in the leaves, which gives information about the level of stress. At a biochemical point of view, we measured the content in enzymes involves in inulin metabolism and sugars synthesis. We observed that the plants suffering from a slight water stress developed better. A simple1-D model was built which describes the root growth in function of the irrigation level and of the soil and atmospheric boundary conditions.

  3. Induction of 1-FEH in Mature Chicory Roots Appears to be Related to Low Temperatures Rather than to Leaf Damage

    Directory of Open Access Journals (Sweden)

    W. Van den Ende

    2002-01-01

    Full Text Available Large-scale inulin production from chicory roots (Cichorium intybus L. is hampered by the induction of 1-FEH activity (fructan 1-exohydrolase and concomitant fructose production in autumn, coincident with a period with low night temperatures that cause leaf damage. To understand whether leaf damage per se is sufficient for 1-FEH induction and fructan breakdown, we defoliated mature chicory plants at a preharvest stage (September 10 and investigated the changes in carbohydrate levels and 1-FEH activities. Also, the activities of 1-SST (sucrose:sucrose 1-fructosyl transferase, EC 2.4.1.99, 1-FFT (fructan:fructan 1-fructosyl transferase, EC 2.4.1.100, and acid invertase (EC 3.2.1.26 were determined. Defoliation did not result in a prompt fructan breakdown and increase in 1-FEH activity, but after 10 days fructan breakdown and 1-FEH activities became higher in the defoliated plants. Defoliation resulted in a sharp decrease in 1-SST activity over the first 24 h. Afterwards, root 1-SST activities of defoliated plants remained at a lower level than in control plants. 1-FFT and invertase activities were not affected by defoliation. It can be concluded that defoliation of plants at the preharvest stage by itself did not induce the same rapid changes as observed in naturally induced October roots by low temperature (harvest stage. Taken together with our finding that 1-FEH is not induced in chicory roots when plants are transferred to the greenhouse early autumn (minimal temperature 14°C, we conclude that low temperatures might be essential for 1-FEH induction.

  4. Effects of mushroom and chicory extracts on the shape, physiology and proteome of the cariogenic bacterium Streptococcus mutans

    Science.gov (United States)

    2013-01-01

    Background Dental caries is an infectious disease which results from the acidic demineralisation of the tooth enamel and dentine as a consequence of the dental plaque (a microbial biofilm) accumulation. Research showed that several foods contain some components with antibacterial and antiplaque activity. Previous studies indicated antimicrobial and antiplaque activities in a low-molecular-mass (LMM) fraction of extracts from either an edible mushroom (Lentinus edodes) or from Italian red chicory (Cichorium intybus). Methods We have evaluated the antimicrobial mode of action of these fractions on Streptococcus mutans, the etiological agent of human dental caries. The effects on shape, macromolecular syntheses and cell proteome were analysed. Results The best antimicrobial activity has been displayed by the LMM mushroom extract with a bacteriostatic effect. At the MIC of both extracts DNA synthesis was the main macromolecular synthesis inhibited, RNA synthesis was less inhibited than that of DNA and protein synthesis was inhibited only by roughly 50%. The partial inhibition of protein synthesis is compatible with the observed significant increase in cell mass. The increase in these parameters is linked to the morphological alteration with transition from cocci of the untreated control to elongated cells. Interestingly, these modifications were also observed at sub-MIC concentrations. Finally, membrane and cytosol proteome analysis was conducted under LMM mushroom extract treatment in comparison with untreated S. mutans cells. Significant changes were observed for 31 membrane proteins and 20 of the cytosol fractions. The possible role of the changed proteins is discussed. Conclusions This report has shown an antibiotic-like mode of action of mushroom and chicory extracts as demonstrated by induced morphogenetic effects and inhibition of specific macromolecular synthesis. This feature as well as the safe use of this extract as result of its natural origin render the

  5. Identification and Characterization of Five BAHD Acyltransferases Involved in Hydroxycinnamoyl Ester Metabolism in Chicory.

    Science.gov (United States)

    Legrand, Guillaume; Delporte, Marianne; Khelifi, Chahinez; Harant, Adeline; Vuylsteker, Christophe; Mörchen, Monika; Hance, Philippe; Hilbert, Jean-Louis; Gagneul, David

    2016-01-01

    Chicory (Cichorium intybus) accumulates caffeic acid esters with important significance for human health. In this study, we aim at a better understanding of the biochemical pathway of these bioactive compounds. Detailed metabolic analysis reveals that C. intybus predominantly accumulates caftaric and chicoric acids in leaves, whereas isochlorogenic acid (3,5-diCQA) was almost exclusively accumulated in roots. Chlorogenic acid (3-CQA) was equally distributed in all organs. Interestingly, distribution of the four compounds was related to leaf age. Induction with methyljasmonate (MeJA) of root cell suspension cultures results in an increase of 3-CQA and 3,5-diCQA contents. Expressed sequence tag libraries were screened using members of the BAHD family identified in Arabidopsis and tobacco as baits. The full-length cDNAs of five genes were isolated. Predicted amino acid sequence analyses revealed typical features of BAHD family members. Biochemical characterization of the recombinant proteins expressed in Escherichia coli showed that two genes encode HCTs (hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferases, HCT1 and HCT2) whereas, three genes encode HQTs (hydroxycinnamoyl-CoA:quinate hydroxycinnamoyltransferases, HQT1, HQT2, and HQT3). These results totally agreed with the phylogenetic analysis done with the predicted amino acid sequences. Quantitative real-time polymerase chain reaction analysis of gene expression indicated that HQT3, HCT1, and HCT2 might be more directly associated with CQA accumulation in cell culture in response to MeJA elicitation. Transient expression of HCT1 and HQT1 in tobacco resulted in a higher production of 3-CQA. All together these data confirm the involvement of functionally redundant genes in 3-CQA and related compound synthesis in the Asteraceae family. PMID:27375627

  6. Identification and characterization of five BAHD acyltransferases involved in hydroxycinnamoyl ester metabolism in chicory

    Directory of Open Access Journals (Sweden)

    Guillaume eLegrand

    2016-06-01

    Full Text Available Chicory (Cichorium intybus accumulates caffeic acid esters with important significance for human health. In this study, we aim at a better understanding of the biochemical pathway of these bioactive compounds. Detailed metabolic analysis reveals that C. intybus predominantly accumulates caftaric and chicoric acids in leaves, whereas isochlorogenic acid (3,5-diCQA was almost exclusively accumulated in roots. Chlorogenic acid (3-CQA was equally distributed in all organs. Interestingly, distribution of the 4 compounds was related to leaf age. Induction with methyljasmonate (MeJA of root cell suspension cultures results in an increase of 3-CQA and 3,5-diCQA contents. Expressed sequence tag libraries were screened using members of the BAHD family identified in arabidopsis and tobacco as baits. The full-length cDNAs of five genes were isolated. Predicted amino acid sequence analyses revealed typical features of BAHD family members. Biochemical characterization of the recombinant proteins expressed in Escherichia coli showed that 2 genes encode HCTs (hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferases, HCT1 and HCT2 whereas 3 genes encode HQTs (hydroxycinnamoyl-CoA:quinate hydroxycinnamoyltransferases, HQT1, HQT2 and HQT3. These results totally agreed with the phylogenetic analysis done with the predicted amino acid sequences. Quantitative real-time polymerase chain reaction analysis of gene expression indicated that HQT3, HCT1 and HCT2 might be more directly associated with CQA accumulation in cell culture in response to MeJA elicitation. Transient expression of HCT1 and HQT1 in tobacco resulted in a higher production of 3-CQA. All together these data confirm the involvement of functionally redundant genes in 3-CQA and related compound synthesis in the Asteraceae family.

  7. Identification and Characterization of Five BAHD Acyltransferases Involved in Hydroxycinnamoyl Ester Metabolism in Chicory

    Science.gov (United States)

    Legrand, Guillaume; Delporte, Marianne; Khelifi, Chahinez; Harant, Adeline; Vuylsteker, Christophe; Mörchen, Monika; Hance, Philippe; Hilbert, Jean-Louis; Gagneul, David

    2016-01-01

    Chicory (Cichorium intybus) accumulates caffeic acid esters with important significance for human health. In this study, we aim at a better understanding of the biochemical pathway of these bioactive compounds. Detailed metabolic analysis reveals that C. intybus predominantly accumulates caftaric and chicoric acids in leaves, whereas isochlorogenic acid (3,5-diCQA) was almost exclusively accumulated in roots. Chlorogenic acid (3-CQA) was equally distributed in all organs. Interestingly, distribution of the four compounds was related to leaf age. Induction with methyljasmonate (MeJA) of root cell suspension cultures results in an increase of 3-CQA and 3,5-diCQA contents. Expressed sequence tag libraries were screened using members of the BAHD family identified in Arabidopsis and tobacco as baits. The full-length cDNAs of five genes were isolated. Predicted amino acid sequence analyses revealed typical features of BAHD family members. Biochemical characterization of the recombinant proteins expressed in Escherichia coli showed that two genes encode HCTs (hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferases, HCT1 and HCT2) whereas, three genes encode HQTs (hydroxycinnamoyl-CoA:quinate hydroxycinnamoyltransferases, HQT1, HQT2, and HQT3). These results totally agreed with the phylogenetic analysis done with the predicted amino acid sequences. Quantitative real-time polymerase chain reaction analysis of gene expression indicated that HQT3, HCT1, and HCT2 might be more directly associated with CQA accumulation in cell culture in response to MeJA elicitation. Transient expression of HCT1 and HQT1 in tobacco resulted in a higher production of 3-CQA. All together these data confirm the involvement of functionally redundant genes in 3-CQA and related compound synthesis in the Asteraceae family. PMID:27375627

  8. Effect of feeding buckwheat and chicory silages on fatty acid profile and cheese-making properties of milk from dairy cows.

    Science.gov (United States)

    Kälber, Tasja; Kreuzer, Michael; Leiber, Florian

    2013-02-01

    Fresh buckwheat (Fagopyrum esculentum) and chicory (Cichorium intybus) had been shown to have the potential to improve certain milk quality traits when fed as forages to dairy cows. However, the process of ensiling might alter these properties. In the present study, two silages, prepared from mixtures of buckwheat or chicory and ryegrass, were compared with pure ryegrass silage (Lolium multiflorum) by feeding to 3 × 6 late-lactating cows. The dietary dry matter proportions realised for buckwheat and chicory were 0.46 and 0.34 accounting also for 2 kg/d of concentrate. Data and samples were collected from days 10 to 15 of treatment feeding. Buckwheat silage was richest in condensed tannins. Proportions of polyunsaturated fatty acids (PUFA) and α-linoleic acid in total fatty acids (FA) were highest in the ryegrass silage. Feed intake, milk yield and milk gross composition did not differ among the groups. Feeding buckwheat resulted in the highest milk fat concentrations (g/kg) of linoleic acid (15.7) and total PUFA (40.5; both P < 0.05 compared with ryegrass). The concentration of α-linolenic acid in milk fat was similar across treatments, but its apparent recovery in milk relative to the amounts ingested was highest with buckwheat. The same was true for the occurrence of FA biohydrogenation products in milk relative to α-linolenic acid intake. Recovery of dietary linoleic acid in milk remained unaffected. Feeding buckwheat silage shortened rennet coagulation time by 26% and tended (P < 0.1) to increase curd firmness by 29%. In conclusion, particularly buckwheat silage seems to have a certain potential to modify the transfer of FA from feed to milk and to contribute to improved cheese-making properties. PMID:23253429

  9. 菊苣体外干物质消化率近红外分析模型的建立%Near-Infrared Reflectance Spectroscopy Analytic Model Established for the IVDMD of Cichorium intybus L

    Institute of Scientific and Technical Information of China (English)

    胡超; 白史且; 张玉; 鄢家俊; 游明鸿; 李达旭; 白玲; 张劲

    2014-01-01

    Chicory (Cichoriumintybus L.)is a new type of forage grasses of high yield and quality with a great value of popular-ization and utilization.In vitro dry matter digestibility (IVDMD)is one of the important indicators of the nutritional value of for-age evaluation.For the study of establishment of Chicory IVDMD NIRS quantitative analysis model,seventy-two species with different genotypes,different growth stages of 204 chicory samples of aboveground material were collected,and by Fourier transform near-infrared diffuse reflectance spectroscopy,through the use of different regression algorithms,can comparing dif-ferent spectral ranges and spectral pretreatment methods,eight chicory IVDMD NIRS calibration models were established,and the best calibration model parameters were chosen.Its calibration coefficient of determination (R2cal )and external validation coef-ficient of determination (R2val)were 0. 953 17 and 0. 904 55,calibration standard deviation (RMSEC)and predictive standard de-viation (RMSEP)was 1. 977 99%and 2. 008 82%,and the correlation coefficient (r)between predicted values and chemical val-ues was 0. 951 08.The results show that using NIRS to determine chicory IVDMD is feasible,and provided a rapid analysis method for the determination IVDMD of chicory.%菊苣(Cichoriumintybus L.)是一种新型的高产优质饲用牧草,具有极大的推广利用价值。体外干物质消化率(in vitro dry matter digestibility,IVDMD)是评价饲草营养价值的重要指标之一。建立菊苣体外干物质消化率的NIRS定量分析模型的研究,采集了72个品种、不同基因型、不同生长发育时期的204个菊苣地上部分为样品,应用傅里叶变换近红外漫反射光谱技术,通过采用不同的回归算法,比较不同的光谱范围和光谱预处理方法,建立了8个菊苣体外干物质消化率(IVDMD)的近红外漫反射光谱校正模型,从中选出最佳参数的校正模型

  10. Desempenho agronômico de quatro cultivares de almeirão Agronomic performance of four chicory cultivars

    Directory of Open Access Journals (Sweden)

    Franciele dos Santos

    2013-03-01

    Full Text Available Na literatura são escassas as informações sobre as principais cultivares de almeirão (Cichorium intybus cultivadas no Brasil. O objetivo do presente trabalho foi avaliar o desempenho agronômico de cultivares de almeirão na região de Campinas-SP. As cultivares avaliadas foram Catalonha, Folha Amarela, Folha Larga e Pão-de-Açúcar, em experimento realizado em canteiros no Centro Experimental Central do Instituto Agronômico, de dezembro de 2009 a janeiro de 2010 com mudas transplantadas. O delineamento experimental no campo foi inteiramente casualizado, com quatro repetições. Aos 50 dias após o transplante foram avaliadas cinco plantas de cada parcela quanto à altura, número de folhas, massa fresca e seca, e em duas dessas plantas avaliou-se a área foliar. Foi avaliado, também, o sabor, com e sem tempero, por seis provadores. As cultivares apresentaram diferenças significativas em relação à altura das plantas, área foliar e sabor. 'Pão-de-Açúcar' apresentou em magnitude maior rendimento agronômico (516,0 g planta-1, seguida pela 'Folha Amarela' (432,7 g planta-1. Esta constitui-se em uma alternativa para o mercado, uma vez que apresenta desempenho semelhante ao das principais cultivares de almeirão comercializadas no Estado de São Paulo.In the literature there is little information about the main chicory cultivars (Cichorium intybus grown in Brazil. The present paper aims to evaluate the agronomic performance of the chicory cultivars in the region of Campinas, São Paulo state, Brazil. Catalonha, Folha Amarela, Folha Larga and Pão-de-Açúcar cultivars were evaluated, in an experiment carried out in field conditions in the Experimental Center of the Instituto Agronômico, from December 2009 to January 2010. The experimental design was completely randomized, with 4 replications. At 50 days after transplanting five plants of each plot were evaluated for the height, leaf number, fresh and dry weight and two of these plants

  11. 晾晒和添加剂对菊苣青贮的影响%Effects of Wilting and Additives on Chicory Silage

    Institute of Scientific and Technical Information of China (English)

    张晓余; 玉柱; 张颖; 许庆方; 朱战波; 马涛

    2015-01-01

    为研究含水量和添加剂对菊苣(Cichorium intybus L)青贮的影响,以开花期菊苣鲜样和晾晒样为原料,分别添加甲酸(6mL/kg)或蔗糖(2%)处理,袋装并密封青贮360d后取样分析。结果表明,经晾晒后制作的菊苣青贮饲料的pH较高,乙酸和氨态氮含量极显著下降(P<0.01);添加甲酸或蔗糖均能够极显著降低菊苣青贮饲料的pH和氨态氮含量(P<0.01),极显著提高乳酸含量(P<0.01);青贮后硝酸盐含量下降。添加剂可以改善菊苣青贮饲料的发酵品质,菊苣经晾晒后再使用添加剂进行青贮效果更好。%Effects of additives and moisture contents on chicory silage were studied. Fresh and wilted chicory in the lfowering stage were placed in sealed vacum plastic bags with formic acid (6mL/kg) or sucrose (2%) and ensiled for 360 days. Results showed that wilting treatment increased pH, and decreased lactic acid and ammonia nitrogen contents significantly(P<0.01). Either formic acid or sucrose decreased pH, acetic acid and ammonia nitrogen contents and increased lactic acid contents of chicory silage (P<0.01). The nitrate content was reduced after ensilaged. Generally, the silage fermentation quality of chicory can be improved by adding either formic acid or sucrose, especially when the material was initially wilted.

  12. ВИВЧЕННЯ ГЕПАТОПРОТЕКТОРНОЇ АКТИВНОСТІ ЕКСТРАКТУ ЦИКОРІЮ (CICHORIUM INTYBUS L.)

    OpenAIRE

    Юсупов, И.; Марупов, Р.; Шукуров, Т.; Давлатмамадова, С.; Бахдавлатов, А.

    2014-01-01

    Method EPR-spectroscopy investigates the maintenance of free radicals in rhizomes, in stalks and leaves wild-growing chicory. According to spectral parametres of signal EPR it is established, that the quantity of the free radicals containing in a chemical compound of chicory depends on a place of growth and the basic parts of a plant of chicory i.e. in leaves more than in a stalk and a root.

  13. Amelioration by chicory seed extract of diabetes- and oleic acid-induced non-alcoholic fatty liver disease (NAFLD)/non-alcoholic steatohepatitis (NASH) via modulation of PPARα and SREBP-1.

    Science.gov (United States)

    Ziamajidi, Nasrin; Khaghani, Shahnaz; Hassanzadeh, Gholamreza; Vardasbi, Safura; Ahmadian, Shahram; Nowrouzi, Azin; Ghaffari, Seyed Mahmood; Abdirad, Afshin

    2013-08-01

    We evaluated the effect of chicory (Cichorium intybus L.) seed extract (CI) on hepatic steatosis caused by early and late stage diabetes in rats (in vivo), and induced in HepG2 cells (in vitro) by BSA-oleic acid complex (OA). Different dosages of CI (1.25, 2.5 and 5 mg/ml) were applied along with OA (1 mM) to HepG2 cells, simultaneously and non-simultaneously; and without OA to ordinary non-steatotic cells. Cellular lipid accumulation and glycerol release, and hepatic triglyceride (TG) content were measured. The expression levels of sterol regulatory element-binding protein-1c (SREBP-1c) and peroxisome proliferator-activated receptor alpha (PPARα) were determined. Liver samples were stained with hematoxylin and eosin (H&E). Significant histological damage (steatosis-inflammation-fibrosis) to the cells and tissues and down-regulation of SREBP-1c and PPARα genes that followed steatosis induction were prevented by CI in simultaneous treatment. In non-simultaneous treatment, CI up-regulated the expression of both genes and restored the normal levels of the corresponding proteins; with a greater stimulating effect on PPARα, CI acted as a PPARα agonist. CI released glycerol from HepG2 cells, and targeted the first and the second hit phases of hepatic steatosis. A preliminary attempt to characterize CI showed caffeic acid, chlorogenic acid, and chicoric acid, among the constituents. PMID:23603006

  14. EFFECTIVENESS OF AUXIN INDUCED IN VITRO ROOT CULTURE IN CHICORY

    OpenAIRE

    Nandagopal, S.; B Ranjitha Kumari

    2007-01-01

    An efficient protocol has been developed for the root culture of (Cichorium intybus L. cv. Focus), the leaf and hypocotyl explants from 25 days old in vitro raised seedlings were cultured on half-strength Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA), α-Napthalenacetic acid (NAA). 0.5 mg/l NAA and 0.1 mg/l IBA induced highest percentage of rooting from matured leaf explants, under total da...

  15. 菊苣航天诱变材料RAPD分析及高产品系筛选%RAPD analysis of Cichorium intybu by space mutagenesis and high-yield strains' selection

    Institute of Scientific and Technical Information of China (English)

    韩永芬; 卢欣石; 舒健虹; 付薇; 陆瑞霞; 覃涛英

    2011-01-01

    Cichorium intybu is a excellent forage with high yield and good palatability, which was introduced from abroad. It has been used in production widely. The genetic relationship and production performance of 29 C. intybu were studied in this research by using RAPD method. The result showed that 15 primers selected from 100 random primers were characteristic of high polymorphism, repeatability and stability. 78 DNA frag ments were amplified, including 63 polymorphic bands. The average percentage of polymorphic bands was 87.77%, cluster analysis showed that genetic similarity coefficient of C. intybus was ranging from 0. 69 to 0. 80, there was a lower genetic diversity among germplasm resources of C. intybus tested. PA-186 clustered a lone. The fingdings indicated an incomplete correlation among the dendrogram and phenotypic characteristics. According to production performance, the yield of twelve new lines were higher than Puna chicory (9. 5 kg/m2) and Jiangjun chicory(9. 7 kg/m2).%菊苣是一种从国外引进的优良牧草品种,产量高、适口性好,现已在生产中广泛应用.该研究以菊苣航天诱变材料和普那、将军2个主推菊苣品种共29份材料为研究对象进行RAPD分析和产量研究.从100个RAPD随机引物中筛选出的多态性强、重复性好且稳定性高的引物15个,15个引物共扩增出78条带,其中多态性带为63条,比例为87.77%.聚类结果表明,所选菊苣材料之间的遗传多样性较低,相似性系数在0.69~0.80,其中材料PA186单成一支,与其他菊苣材料相似性较低.材料之间的聚类关系与表型特征呈不完全的相关性.从菊苣牧草生产性能看,产量高于目前主推品种黔引普那菊苣(9.5 kg/m2)和将军(9.7 kg/m2)的品系有12个.

  16. Fatty acid content and lipid fractions in herbs

    DEFF Research Database (Denmark)

    Petersen, Majbritt Bonefeld; Søegaard, Karen; Jensen, Søren Krogh

    2012-01-01

    ten species were: chicory (Cichorium intybus), ribwort plantain (Plantago lanceolata), salad burnet (Sanguisorba minor), birds-foot trefoil (Lotus corniculatus), white meliot (Melilotus officinalis), caraway (Carum carvi), alfalfa (Medicago sativa), chervil (Anthriscus cerefolium), white clover...

  17. 干旱胁迫条件下菊苣的光合响应%Photosynthetic properties of three chicory lines and their responses to drought stress

    Institute of Scientific and Technical Information of China (English)

    韩永芬; 罗天琼; 李娟; 赵相勇; 杨菲; 陆瑞霞

    2013-01-01

    研究了3种具有不同抗旱性的菊苣(Cichorium intybus)[航天诱变新品系PA-43、PA-82和亲本普那菊苣]的光合特性及对干旱胁迫的响应.结果表明,3个菊苣品系的净光合速率(Pn)、蒸腾速率(Tr)和气孔导度(Cond)日变化趋势都是双峰曲线.午间有明显“午休”现象,且导致午休的主要原因是非气孔因素.PA-82的Pn、Tr、Cond值均最高,PA-43的相应值均最小;3个菊苣品系的胞间CO2浓度(Ci)在10:00-12:00时比较低,其变化曲线与Pn相反.水分利用效率(WUE)以PA 82为最高,普那菊苣居中,PA-43为最低.随着干旱胁迫程度的加剧,3个菊苣品系的Pn、Tr、Cond、暗适应下PSⅡ最大光化学效率(Fv/Fm)、暗适应下PSⅡ的潜在活性(Fv/Fo)、光化学猝灭系数(qP)均有一定程度的降低.其中,PA-82下降速度慢、幅度小,PA-43下降速度快、幅度大.Ci、qN值随着胁迫程度的加剧呈上升趋势.其中,PA 82上升速度慢、幅度小,PA-43上升速度快、幅度大.相对于抗旱性弱的菊苣PA-43、抗旱性强的菊苣PA 82、普那菊苣在受到严重干旱胁迫时,具有较强的抵抗干旱的能力.%The photosynthetic properties of three chicory (Cichorium intybus) lines were investigated under water stress through pot experiment.The lines which included space-mutagenesis-bred lines(PA-82 and PA-43),and their initial strain (Puna chicory) were studied using Li-6400 portable photosynthesis system.The results showed that,diurnal net photosynthetic rate (Pn),transpiration rate (Tr),and stomatal conductance (Cond) presented as double-peak curves.There was an obvious mid-day Pn depression,which was caused by nonstomatal factors.The highest Pn,Tr and Cond values were observed in PA-82,and the lowest corresponding values were observed in PA-43 (P<0.05).Intercellular CO2 concentration (Ci) of three lines showed an opposite course to that of Pn,which reached its valley at 10:00-12:00.PA-82 had the highest water use efficiency

  18. Desempenho de três cultivares de almeirão sob cultivo protegido Performance of three chicory cultivars grown in greenhouse

    Directory of Open Access Journals (Sweden)

    Maria do Carmo S.S. Novo

    2003-03-01

    Full Text Available O almeirão (Cichorium intybus L. é uma Asteraceae muito semelhante à chicória da qual se diferencia por possuir folhas mais alongadas, mais estreitas, recobertas por pelos e com sabor amargo mais pronunciado. Avaliou-se o desempenho das cultivares Folha Larga, Pão-de-Açúcar e Catalonha, em estufa com cobertura plástica, em experimento, de julho a setembro/2001, no Núcleo Experimental de Campinas do IAC. O experimento foi disposto em blocos ao acaso, com parcelas subdivididas para época de avaliação, tendo três repetições. Aos 50; 57; 64 e 71 dias após a semeadura foram avaliadas dez plantas de cada subparcela quanto à altura, número médio de folhas, biomassas de matéria fresca e seca e área foliar. Nessas oportunidades foram também avaliadas as produções de folhas em um metro linear de canteiro. Não houve diferença entre as cultivares quanto ao número médio de folhas por planta porém houve interação entre cultivares e época de avaliação para área foliar, biomassas fresca e seca e produção de folhas. A cultivar Catalonha foi a que apresentou maior altura e maior produtividade, embora as biomassas de matéria fresca e seca tenham sido superiores na Pão-de-Açúcar que também apresentou maior área foliar. Recomenda-se, com base na área foliar, a colheita dessas hortaliças até os 64 dias. Concluiu-se que foi viável a produção de almeirão sob cultivo protegido constituindo-se em mais uma alternativa para esse sistema de produção.Cichorium intibus L. is an Asteraceae very similar to endive, distinguished by longer and narrower leaves covered with pile and bitter flavored. An experiment was carried out in a plastic greenhouse, to evaluate the performance of the Folha Larga, Pão-de-Açúcar and Catalonha cultivars, from July to September/2001, at IAC, Campinas, Brazil. A split plot design was used with the cultivars in the main plots and sampling data as subplots. Each main plot and subplot was

  19. Non-traditional Forages in a Managed Grazing System for Control of Gastrointestinal Parasites in Sheep: Preliminary Work

    Science.gov (United States)

    This project compared lambs grazing forage chicory (Cichorium intybus L.) with lambs grazing brown mid-rib forage sorghum (Sorghum bicolor (L.) Moench.) x sudangrass (Sorghum sudanense Piper) hybrid (BMR) to determine if anti-parasitic effects of chicory could be demonstrated. Lambs grazed these fo...

  20. 60Co-γ辐射对菊苣种子发芽及幼苗生理的影响%Effects of 60Co-γ-rays Irradiation on Chicory Seed Germination and Seedling Physiology

    Institute of Scientific and Technical Information of China (English)

    张玉; 白史且; 李达旭; 王曾珍

    2013-01-01

    Chicory seeds were irradiated with 60Co-γ-rays at 100, 150, 200 and 300 Gy to determine germination rate and physiological characteristics from mutagenic effects of irradiation doses. Results showed that seed germination rate decreased as the irradiation dosage increased. There was significant difference between 200 Gy irradiation dosage and control(P<0. 05). Superoxide dismutase (SOD) activities, peroxi-dase (POD) activities, soluble protein and soluble sugar contents first increased then declined with a gradual irradiation increase, and differed significantly between control and 150 Gy, 100 Gy, 150 Gy, 150 Gy treatments (P<0. 05), respectively. MDA content increased with increased irradiation dosage, and there was significant difference between control and the treatments above 150 Gy dose (P<0. 05). The protein content first increased then declined, and differed significantly between control and treatments (P<0. 05). It was concluded that the mutagenic effect of chicory irradiated by low dosage (below 150 Gy) was not obvious. However, physiological and biochemical characteristics of chicory irradiated above 200 Gy were significantly altered. The study provides a theoretical basis for chicory radiation mutation breeding.%为明确60 Co-γ射线对菊苣(Cichorium intybus L.)辐照诱变效果,采用不同剂量60Co-γ射线(100 Gy,150 Gy,200 Gy和300 Gy)辐照处理菊苣种子,测定其发芽率和相关生理指标.结果表明:随着辐照剂量的增加,菊苣发芽率降低,200 Gy以上辐照处理发芽率与对照差异显著(P<0.05);超氧化物歧化酶(SOD)、过氧化物酶(POD)活性、可溶性蛋白和可溶性糖含量随着辐照剂量增加均表现为先升后降的趋势,分别在辐射剂量为150 Gy,100 Gy,150Gy,150 Gy及以上时与对照差异显著(P<0.05);丙二醛(MDA)含量随着辐照剂量增加而逐渐升高,150 Gy以上辐照处理与对照差异显著(P<0.05);蛋白含量呈现先增后减的趋

  1. СОДЕРЖАНИЕ НЕКОТОРЫХ МИКРОЭЛЕМЕНТОВ В ПОЧВЕ И ТКАНЯХ ЦИКОРИЯ (CICHORIUM INTYBUS)

    OpenAIRE

    Евдокимова, Р.; Каримова, А.

    2013-01-01

    В данной работе мы исследовали химический состав растения цикория (Cychorium intybus L.), произрастающего в Оренбургской области. По результатам нашего исследования было выявлено, что в цикории содер-жатся разные эссенциальные микроэлементы. Среди них можно выделить как полезные, например, кобальт, так и токсичные – кадмий....

  2. Introduction of TaNHX2 gene enhanced salt tolerance of transgenic puna chicory plants%导入TaNHX2基因提高了转基因普那菊苣的耐盐性

    Institute of Scientific and Technical Information of China (English)

    张丽君; 程林梅; 杜建中; 郝曜山; 王亦学; 李贵全; 孙毅

    2011-01-01

    The growing severity of land salinization and alkalization in some areas of China has resulted in adverse impacts on crop production and agriculture-ecological environment. Soil salinity is one of the major limiting factors affecting crop growth, development and yield in arid and semiarid regions of China. Therefore, breeding for saline and alkaline tolerant crop varieties through genetic engineering approaches is of great significance for improving the crop survivability in saline and alkaline conditions and ecological environment, as well as for enhancing crop production. With the development of molecular biology and genetic transformation technology, numerous genes related to saline and alkaline tolerances have been found and cloned. Introduction of these genes into various plants has achieved rapid improvement of their tolerances saline and alkaline conditions. Cichorium intybus L. Cv. Puna, belonging to Asteraceae family is commonly known as witloof cichory and widely distributed in northern, and central China. In this study, a wheat ( Triticum aestivum Linn) vacuolar Na+/H+ exchanger gene ( TaNHX2) , in connection with plant salt tolerance, was introduced into puna chicory using Agrobacterium-mediated transformation approach. Puna chicory leaf segments were precultured for 2-3 days before being co-cultivated with the Agrobacterium strain, pBin438-TaNHX, which containing the target gene2 Transformed buds were selected on the MS medium containing kanamycin ( 80mg/L) and Cefalexin (l000mg/L) , and 28 putative transgenic lines were obtained and used for further molecular and biological assay. Analysis of the transgenic plants was performed by using PCR and Southern blot hybridization, which proved that the wheat vacuolar Na+/H+ exchanger gene was successfully integrated into puna chicory genome. Effects of NaCl concentration on growth of wild type and T0 seed germination, callus induction and seedling growth were investigated. The results indicated that

  3. Impact of PAHs on the development of the arbuscular mycorrhizal fungus, G. Intraradices, on the colonization of chicory and carrot grown in vitro

    Energy Technology Data Exchange (ETDEWEB)

    Verdin, A.; Lounes-Hadj Sahraoui, A.; Fontaine, J.; Grandmougin-Ferjani, A.; Durand, R. [Universite du Littoral-Cote d' Opale, Lab. de Mycologie/Phytopathologie/Environnement, 62 - Calais (France)

    2005-07-01

    Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous and persistent pollutants found in many environments as result of the incomplete combustion of organic matter, and some of them are of great environmental concern due to their highly cytotoxic, genotoxic and carcinogenic properties for mammals. PAHs are thermodynamically stable and recalcitrant to microbial degradation, due to their aromatic nature and low aqueous solubility. Ecologically and economically speaking, plants have tremendous potential for bio-remediation of PAH-contaminated soils. The effect of plant roots on the dissipation of organic pollutants has mainly been attributed to an increase in microbial population and selection of specialized microbial communities in the rhizosphere, and also by improving physical and chemical soil conditions. Arbuscular mycorrhizal (AM) fungi living in symbiosis with plant roots play an essential role in plant nutrition and stress tolerance. AM plants are known to be involved in the biodegradation of pollutants such as PAHs. The role of AM fungi concerns two aspects: the improvement of the establishment and development of plants on polluted soil and the enhancement of PAHs degradation levels. AM colonization of different plant species is negatively affected when the plants are grown in contaminated soils. Nevertheless the AM colonization was shown to enhance plant survival and growth. Objectives of this work was to study the impact of PAHs on the development of G. intraradices and on the colonization of chicory (Cichorium intybus L.) and carrot (Daucus carota L.) roots transformed by Agrobacterium rhizogenes. Monoxenous root cultures have obvious advantages over traditional systems. This technique provides unique visualization of extra-radical fungus development and also allows an important production of extra-radical hyphae, spores and colonized roots free of any other microorganisms. These aspects are important to evaluate direct impact of PAHs on AM fungal

  4. Impact of PAHs on the development of the arbuscular mycorrhizal fungus, G. Intraradices, on the colonization of chicory and carrot grown in vitro

    International Nuclear Information System (INIS)

    Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous and persistent pollutants found in many environments as result of the incomplete combustion of organic matter, and some of them are of great environmental concern due to their highly cytotoxic, genotoxic and carcinogenic properties for mammals. PAHs are thermodynamically stable and recalcitrant to microbial degradation, due to their aromatic nature and low aqueous solubility. Ecologically and economically speaking, plants have tremendous potential for bio-remediation of PAH-contaminated soils. The effect of plant roots on the dissipation of organic pollutants has mainly been attributed to an increase in microbial population and selection of specialized microbial communities in the rhizosphere, and also by improving physical and chemical soil conditions. Arbuscular mycorrhizal (AM) fungi living in symbiosis with plant roots play an essential role in plant nutrition and stress tolerance. AM plants are known to be involved in the biodegradation of pollutants such as PAHs. The role of AM fungi concerns two aspects: the improvement of the establishment and development of plants on polluted soil and the enhancement of PAHs degradation levels. AM colonization of different plant species is negatively affected when the plants are grown in contaminated soils. Nevertheless the AM colonization was shown to enhance plant survival and growth. Objectives of this work was to study the impact of PAHs on the development of G. intraradices and on the colonization of chicory (Cichorium intybus L.) and carrot (Daucus carota L.) roots transformed by Agrobacterium rhizogenes. Monoxenous root cultures have obvious advantages over traditional systems. This technique provides unique visualization of extra-radical fungus development and also allows an important production of extra-radical hyphae, spores and colonized roots free of any other microorganisms. These aspects are important to evaluate direct impact of PAHs on AM fungal

  5. РАСПРЕДЕЛЕНИЕ МИКРОЭЛЕМЕНТОВ В ПОЧВЕ И ТКАНЯХ ЦИКОРИЯ (CICHORIUM INTYBUS L.)

    OpenAIRE

    Евдокимова, Р.; Каримова, А.

    2013-01-01

    В данной работе мы исследовали химический состав растения цикория (Cychorium intybus L.), произрастающего в Оренбургской области. По результатам нашего исследования было выявлено, что в цикории содержатся очень важные эссенциальные микроэлементы (Zn, Сu, Сr, Co). Причем распределены они неравномерно. Более того было выявлено что для некоторых из них существует некий барьер поступления в генеративные и ассимилирующие органы растения....

  6. Effect of the Aqueous Extracted from Chicory Root on the Seed Vigor of Three Herbs%菊苣根水浸提液对3种牧草种子活力的影响

    Institute of Scientific and Technical Information of China (English)

    解宝; 吴春会; 王佺珍

    2011-01-01

    [目的]探讨菊苣根水浸提液对牧草种子萌发及幼苗生长的影响.[方法]采用不同浓度(0、12.5和50.0g/L)的菊苣(Cichorium intybus L.)根水浸提液,处理高羊茅(Festuca arundinacea L.)、白三叶(Trifolium repens L.)和紫花苜蓿(Medicago sativaL.)等3种牧草种子.[结果]菊苣根水浸提液对3种牧草种子的种子发芽率、发芽指数、活力指数、幼苗根长及芽长生长主要表现为抑制作用,且随水浸提液浓度增加而抑制效应增强;同浓度下,高羊茅种子萌发受到的抑制效应最强.不同植物幼根和幼茎对水浸提液的敏感性不同;高羊茅的幼芽和幼根对菊苣根水浸提液极为敏感,在50.0 g/L浓度下,高羊茅的幼芽和幼根抑制率为39.96%和38.83%.[结论]砷开发植物源除草剂对杂草的防治和莉苣综合治理的研究具有一定的参考价值.%[Objective] The effect of the aqueous extracted from Cichorium intybus L. Root on seed germination and seedling growth was investigated. [ Method] The different concentrations(0, 12.5 and 50.0 g/L) of aqueous extract were adopted to process the seeds of Festuca anindinacea L , Trifolium repens L. And Medicago sativa L. [ Results ] The results showed that the aqueous extract of chicory root had certain influence on herb seed germination and seedling growth, namely, the inhibition of germination index, vigor index, seedling root and bud growth was strengthened with an increment of aqueous extract concentration and under the condition of same extract concentration, the seed germination of festuca would suffer the strongest inhibition effect. The different plant seedling root and stem had different sensitivity to aqueous extract and seedling root and stem of festuca were extraordinary sensitive to aqueous extract, which inhibition rate reached 39.96% and 38.83% in SO. Og/L of the extract concentration. [ Conclusion ] The reference value for the development of plant type herbicide in comprehensive

  7. Establishment and Optimization of Puna Chicory Genetic Transformation System with Agrobacterium-mediated Method%农杆菌介导普那菊苣遗传转化体系的建立

    Institute of Scientific and Technical Information of China (English)

    张丽君; 程林梅; 杜建中; 李贵全; 孙毅

    2011-01-01

    以普那菊苣(Cichorium intybus L.cv.Puna)叶片为试验材料,接种于含不同激素浓度配比的MS培养基上进行愈伤组织、芽分化以及根再生的诱导,分析了不同激素浓度及其配比对愈伤组织诱导和芽分化以及根再生效果的影响.以已经建立的再生体系为基础,以农杆菌菌株LBA4404(含质粒pBin438- TaNHX2)侵染转化普那菊苣,探索普那菊苣高效遗传转化体系.结果表明:对外植体适宜的预培养时间为2~3 d,与农杆菌的共培养时间也应控制在2~3 d;侵染时间控制在8 min左右;卡那霉素(Km)阳性筛选的适宜选择浓度为60mg·L-1.乙酰丁香酮(AS)200 μmol·L-1是促进农杆菌转化的最佳浓度,200 W超声波处理、20次负压处理也可提高农杆菌转化率效果.26 mg·L- 1 Km是野生型普那菊苣苗能够存活的上限,头孢唑林钠和头孢噻肟钠在500~1000 nmg·L-1浓度范围内、羧苄青霉素300 mg·L-1和氨苄青霉素在40~60 mg·L-1浓度范围内均能较好的诱导出愈伤组织和芽.将来自小麦(Triticum aestivum)的Na+/H+逆向转运蛋白(vacuolar Na+/H+ exchanger or antiporter,简称NHX,NHE或NHA)导入普那菊苣;经抗生素筛选以及针对TaNHX2基因的PCR检测和Southern杂交分析,证明获得了28株转TaNHX2基因的普那菊苣植株.%Chicory (Cichorium intybus L. Cv. Puna) leaf segments from aseptic seedlings were used as experimental materials. The explants were inoculated onto the MS medium with various phytohormone combinations to induce callus formation, and bud and root regeneration. Effects of phytohormone concentrations and combinations on the induction of callus, buds and roots were analyzed. Agrobacterium tumefa-ciens LBA4404 (harboring plasmid pBin438-TaNHX2) was used to infect Puna Chicory explants based on the regeneration system that had been established for the high efficiency transformation of the cultivar. Result showed that both suitable pre-culture time and co

  8. EFFECTIVENESS OF AUXIN INDUCED IN VITRO ROOT CULTURE IN CHICORY

    Directory of Open Access Journals (Sweden)

    S Nandagopal

    2007-11-01

    Full Text Available An efficient protocol has been developed for the root culture of (Cichorium intybus L. cv. Focus, the leaf and hypocotyl explants from 25 days old in vitro raised seedlings were cultured on half-strength Murashige and Skoog (MS medium supplemented with different concentrations and combinations of Indole-3-acetic acid (IAA, Indole-3-butyric acid (IBA, α-Napthalenacetic acid (NAA. 0.5 mg/l NAA and 0.1 mg/l IBA induced highest percentage of rooting from matured leaf explants, under total dark condition. After three weeks well established roots were separated. Fresh root tissue, in amount of 0.5 was subcultured in half-strength MS liquid medium supplemented with 0.2 mg/l NAA and 0.5 mg/l IBA, under continuous agitation at 110 rpm and total dark condition. The biomass of root culture was increased to 5.820 g after 6 weeks of culture. The root culture was maintained up to the 8 weeks.

  9. Growth and chemical composition of chicory and performance of lambs grazing chicory relative to grass–clover mixtures

    OpenAIRE

    Kidane, Alemayehu; Sørheim, Kristin; Eik, Lars Olav; Steinshamn, Håvard

    2014-01-01

    We report results from trials on chicory growth (experiment 1) and lamb growth on chicory (experiment 2). In experiment 1, we assessed yield and chemical composition on swards established under organic conditions as perennial ryegrass/red clover, perennial ryegrass/chicory, chicory/red clover (CHRC), pure perennial ryegrass (RG-alone) and pure chicory (CHalone) over two years. Chicory/red clover and CH-alone showed improved crude protein, Ca, Mg, P, Cu and net energy contents comp...

  10. EPR-spectroscopic properties of chicory

    International Nuclear Information System (INIS)

    By means of electron paramagnetic resonance-spectroscopy the containing of free radicals in rhizomes, leaves and stems of chicory is studied. According to spectral parameters of EPR signal it is defined that the quantity of free radicals containing in rhizomes, leaves and stems of chicory depends on its growth place and ecological condition of environment.

  11. Forage herbs improve mineral composition of grassland herbage

    DEFF Research Database (Denmark)

    Pirhofer-Walzl, Karin; Søegaard, Karen; Jensen, Henning Høgh;

    2011-01-01

    groups (grasses, legumes and herbs). Herb species included chicory (Cichorium intybus L.), plantain (Plantago lanceolata L.), caraway (Carum carvi L.) and salad burnet (Sanguisorba minor L.). We also investigated the effect of slurry application on the macro- and micromineral concentration of grasses...

  12. Long-Term Cultured Hairy Roots of Chicory—A Rich Source of Hydroxycinnamates and 8-Deoxylactucin Glucoside

    OpenAIRE

    Malarz, Janusz; Stojakowska, Anna; Kisiel, Wanda

    2013-01-01

    A 12-year-old hairy root culture of Cichorium intybus L., a callus culture of the plant as well as roots and leaves of a wild plant of chicory, and roots of two C. intybus L. var. sativum cultivars were examined in respect of their hydroxycinnamate and sesquiterpene lactone compositions and contents. Total phenolics and diphenylpicrylhydrazyl radical scavenging activity of the examined plant tissues were also analyzed. The most active in radical scavenging were extracts from the hairy roots a...

  13. Herbage intake and milk production of late-lactation dairy cows offered a second-year chicory crop during summer.

    Science.gov (United States)

    Muir, S K; Ward, G N; Jacobs, J L

    2015-12-01

    Chicory (Cichorum intybus L.) is a summer-active forage herb which has been proposed as an option to increase summer feed supply, increase dry matter intake, nutrient intake, and milk yield from nonirrigated dairy production systems in southern Australia. Dry matter intake, nutrient intake, milk yield, and yield of milk fat and protein of predominantly Holstein-Friesian dairy cows in late lactation consuming 3 herbage-based diets (4 replicates per treatment) were measured. The 3 grazed herbages were second-year chicory (CHIC) and perennial ryegrass (Lolium perenne L.; PRG) monocultures and a mixed sward (~50:50) of chicory and perennial ryegrass (MIX). All diets (CHIC, PRG, and MIX) were supplemented with alfalfa (Medicago sativa L.) hay (5.5kg of DM/cow per day) and an energy-based concentrate pellet (4.0kg of DM/cow per day). There were no significant differences in milk yield (12.0 to 12.6kg/d across the treatments) or the yield of milk fat (539 to 585g/d) and milk protein (433 to 447g/d) between the 3 herbage-based diets. No differences in DMI (17.9 to 19.2kg/d) or estimated metabolizable energy intake (173 to 185MJ/d) were noted between treatments. Estimated metabolizable energy concentrations in the forages on offer were lower in CHIC than PRG (7.6 vs. 8.2MJ/kg of dry matter), but the concentration in consumed herbage was not different (9.1 vs. 9.2MJ/kg of dry matter); as such, potential for increased milk yield in cows offered CHIC was limited. Increased concentration of polyunsaturated fatty acids was observed in chicory herbage compared with perennial ryegrass. This was associated with increased milk conjugated linoleic acid and milk polyunsaturated fatty acids when chicory formed part of the diet (CHIC compared to PRG and MIX). Chicory could be used as an alternative to perennial ryegrass in summer; however, the developmental stage of chicory will influence concentrations of metabolizable energy and neutral detergent fiber and, therefore, intake and milk

  14. Dicaffeoylquinic Acid-Enriched Fraction of Cichorium glandulosum Seeds Attenuates Experimental Type 1 Diabetes via Multipathway Protection.

    Science.gov (United States)

    Tong, Jing; Ma, Bingxin; Ge, Lanlan; Mo, Qigui; Zhou, Gao; He, Jingsheng; Wang, Youwei

    2015-12-23

    Chicory has a major geographical presence in Europe and Asia. Cichorium glandulosum Boiss. et Huet, a genus Cichorium, is used for medicinal and food purposes in Asia. In this study, a dicaffeoylquinic acid-enriched fraction of C. glandulosum seeds n-BuOH fraction (CGSB) could ameliorate type 1 diabetes mellitus (T1DM) in streptozotocin (STZ)-induced diabetic mice with continuous administration for 2 weeks. CGSB treatment showed significantly higher plasma insulin levels but lower free fatty acids in adipose tissue and liver. Moreover, CGSB improved pancreatic islet mass. In vitro, different fractions of C. glandulosum seed (CGS) induced the differentiation of 3T3-L1 preadipocytes. The mRNA level for peroxisome proliferator-activated receptor alpha increased in high glucose treatment group in HepG2 cells, while CGSB significantly down-regulated the mRNA expression. The main compound of CGSB, 3,5-dicaffeoylquinic acid, was isolated and identified, which exhibited α-glucosidase inhibitory activity. These findings demonstrated that CGSB attenuated experimental T1DM via multipathway protection. PMID:26586022

  15. Toxicological evaluation of a chicory root extract

    OpenAIRE

    Schmidt, Barbara M.; Ilic, Nebojsa; Poulev, Alexander; Raskin, Ilya

    2007-01-01

    An Ames test and a 28-day sub-chronic toxicity study in male and female Sprague–Dawley rats were conducted to evaluate the safety of a chicory root extract being investigated as a therapeutic for inflammation. Chicory extract had no mutagenic activity in the Ames test although it was cytotoxic to certain strains of Salmonella at higher doses with and without metabolic activation. For the 28-day rat study, measurements included clinical observations, body weights, food consumption, clinical pa...

  16. A study on bacterial softrot in witloof chicory.

    OpenAIRE

    Schober, B.M.

    1998-01-01

    The production process of witloof chicory, which consists of the root production stage, the storage of the roots and the forcing of the chicory heads, was monitored for the presence of softrot bacteria. In the Netherlands, bacterial softrot in chicory is caused by Erwinia carotovora subsp . carotovora ( Ecc ) and Pseudomonas marginalis .Populations of these species colonised the chicory leaves during the root production stage causing softrot only in the forcing stage. The colonisation inciden...

  17. A study on bacterial softrot in witloof chicory.

    NARCIS (Netherlands)

    Schober, B.M.

    1998-01-01

    The production process of witloof chicory, which consists of the root production stage, the storage of the roots and the forcing of the chicory heads, was monitored for the presence of softrot bacteria. In the Netherlands, bacterial softrot in chicory is caused by Erwinia carotovora subsp . carotovo

  18. Effects of Genotype, Seed Age and KNO3 on Germination of Radicchio (Cichorium intybus L. and Endive (Cichorium endivia L. Seed

    Directory of Open Access Journals (Sweden)

    Dijana Ocvirk

    2013-01-01

    Full Text Available After purchasing seed it often happens that all the seed is not used during one season, but they are stored for a couple of years. The aim of this study was to examine whether germination of radicchio and endive is still high enough after longer storage. We also wanted to define seed vigor in relation to the year of production and variety, and whether pretreatment with KNO3 could improve germination of older seed of radicchio and endive.The seed of three varieties of radicchio (‘Pallarossa’, ‘Verona’ and ‘Pandizucchero’ and three varieties of endive (‘Dječja glava’, ‘Pankalierka’ and ‘Escariol žuta’, from five seasons of certification (06/07, 07/08 08/09, 09/10 and 10/11 was tested.The following seed traits were analyzed: 1000 seed weight, moisture, germination rate, standard germination test, seed electrical conductivity, and the amount of absorbed water per gram of seed for 24 hours. Seed was germinated in two soaking treatments: in tap water with cooling pre-treatment, and KNO3. Based on the results, it was concluded that treatment with KNO3 significantly increases the standard germination of radicchio, as compared with water, and does not improve the germination of endive seed, but on the contrary, it has a negative effect. In both plant species, conductivity does not increase with seed age, because the oldest seed did not show the highest EC.In order to fully assess the impact of aging on seed vigor in tested plant species it is necessary to conduct further research.

  19. Effects of Genotype, Seed Age and KNO3 on Germination of Radicchio (Cichorium intybus L. and Endive (Cichorium endivia L. Seed

    Directory of Open Access Journals (Sweden)

    Dijana Ocvirk

    2013-03-01

    Full Text Available Normal 0 false false false HR ZH-CN X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Calibri","sans-serif"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:HR; mso-fareast-language:HR;} After purchasing seed it often happens that all the seed is not used during one season, but they are stored for a couple of years. The aim of this study was to examine whether germination of radicchio and endive is still high enough after longer storage. We also wanted to define seed vigor in relation to the year of production and variety, and whether pretreatment with KNO3 could improve germination of older seed of radicchio and endive.The seed of three varieties of radicchio (‘Pallarossa’, ‘Verona’ and ‘Pandizucchero’ and three varieties of endive (‘Dječja glava’, ‘Pankalierka’ and ‘Escariol žuta’, from five seasons of certification (06/07, 07/08 08/09, 09/10 and 10/11 was tested.The following seed traits were analyzed: 1000 seed weight, moisture, germination rate, standard germination test, seed electrical conductivity, and the amount of absorbed water per gram of seed for 24 hours. Seed was germinated in two soaking treatments: in tap water with cooling pre-treatment, and KNO3. Based on the results, it was concluded that treatment with KNO3 significantly increases the standard germination of radicchio, as compared with water, and does not improve the germination of endive seed, but on the contrary, it has a negative effect. In both plant species, conductivity does not increase with seed age, because the oldest seed did not show the highest EC.In order to fully assess the impact of aging on seed vigor in tested plant species it is necessary to conduct further research.

  20. Increasing of antioxidant and superoxide dismutase activity in chicory transgenic plants

    Directory of Open Access Journals (Sweden)

    Matvieieva N. A.

    2013-03-01

    Full Text Available Aim. Determination of the antioxidant activity (AOA and superoxide dismutase (SOD activity in transgenic chicory plants carrying the human interferon α2b target and nptII or bar selective genes. Methods. AOA was measured by a method based on the determination of kinetics of the reduced 2,6-dichlorophenolindophenol oxidation. SOD activity was assayed using the system consisting of ethionine, riboflavin, and nitroblue tetrazolium. Results. Antioxidant activity of transformed plants extracts was more than 1,91–2,59 and 2,04–2,43 times over the activity of control non-transgenic plants (at nptII and bar gene presence respectively. SOD activity was higher in transgenic plants than in the control, and was 2,03 ± 0,46–3,33 ± 0,54 U/g weight (nptII gene and 2,25 ± 0,46–2,68 ± 0,08 U/g weight (bar gene. Conclusions. Transgenic C. intybus plants have higher antioxidant and superoxide dismutase activity compared to non-transgenic plants. The increasing of AOA and SOD activity is a response of plants to transformation stress factor and integration of foreign genes in plant genome.

  1. Action of Chicory Fructooligosaccharides on Biomimetic Membranes

    OpenAIRE

    Barbosa, A. F.; Henrique, R. S.; A. S. Lucho; V. Paffaro; J.M. Schneedorf

    2014-01-01

    Fructooligosaccharides from chicory (FOSC) are functional prebiotic foods recognized to exert several well-being effects in human health and animal production, as decreasing blood lipids, modulating the gut immune system, enhancing mineral bioavailability, and inhibiting microbial growth, among others. Mechanisms of actions directly on cell metabolism and structure are however little known. In this sense this work was targeted to investigate the interaction of FOSC with biomimetic membranes (...

  2. Control of Phoma exigua in Witloof chicory

    OpenAIRE

    Kruistum, van, G.

    2013-01-01

    Applied Plant Research (AGV Research Unit) tested after root harvesting and during forcing experiments the efficacy of the fungicide Tecto SC (active ingredient: thiabendazole) of Syngenta Crop protection against Phoma exigua in witloof chicory (chicon) production. The trial was carried out in three different dosages with one fungicide Tecto SC as a post harvest treatment on taproots. The fungicide Rovral SC (active ingredient: Iprodione) acted as reference. The results were compared to a non...

  3. Preparation and application of molecularly imprinted polymer for isolation of chicoric acid from Chicorium intybus L. medicinal plant.

    Science.gov (United States)

    Saad, Engy M; Madbouly, Adel; Ayoub, Nahla; El Nashar, Rasha Mohamed

    2015-06-01

    Molecularly imprinted polymer (MIP) was synthesized and applied for the extraction of chicoric acid from Chicory herb (Chicorium intybus L.). A computational study was developed to find a suitable template to functional monomer molar ratio for MIP preparations. The molar ratio was chosen based on the comparison of the binding energy of the complexes between the template and functional monomers. Based on the computational results, eight different polymers were prepared using chicoric acid as the template. The MIPs were synthesized in a non-covalent approach via thermal free-radical polymerization, using two different polymerization methods, bulk and suspension. Batch rebinding experiments were performed to evaluate the binding properties of the imprinted polymers. The best results were obtained with a MIP prepared using bulk polymerization with 4-vinylpyridine (4-VP) as the functional monomer and ethylene glycol dimethacrylate (EGDMA) as the crosslinker with a molar ratio of 1:4:20. The best MIP showed selective binding ability toward chicoric acid in the presence of the template's structural analogues, caffeic acid, caftaric acid and chlorogenic acid. PMID:26002213

  4. Thrombosis preventive potential of chicory coffee consumption: a clinical study

    OpenAIRE

    Schumacher, Edit; Vigh, Éva; Molnár, Valéria; Kenyeres, Péter; Fehér, Gergely; Késmárky, Gábor; Tóth, Kálmán; Garai, János

    2011-01-01

    Abstract Protective effects of plant polyphenol intake on cardiovascular morbidity and mortality are widely acknowledged. Caffeine-free chicory coffee is a rich source of plant phenolics, including caffeic acid, which inhibits in vitro platelet aggregation, and also phenylpyruvate tautomerase enzymatic activity of the proinflammatory cytokine, macrophage migration inhibitory factor (MIF). To assess whether chicory coffee consumption might confer cardiovascular benefits we performed...

  5. Interrelations between Herbage Yield, α-Tocopherol, β-Carotene, Lutein, Protein, and Fiber in Non-Leguminous Forbs, Forage Legumes, and a Grass−Clover Mixture as Affected by Harvest Date

    DEFF Research Database (Denmark)

    Elgersma, Anjo; Søegaard, Karen; Jensen, Søren Krogh

    2015-01-01

    Pastures with diverse botanical composition may enhance animal-derived product quality. A recent study demonstrated high vitamin concentrations and yields in some forb species. The objectives of the present study were to investigate interrelations between herbage yields, vitamin concentrations......, protein and fiber contents and analyze the effect of harvest date. We hypothesized that interrelations would be similar across investigated forage species. Four nonleguminous forbs: salad burnet (Sanguisorba minor), caraway (Carum carvi), chicory (Cichorium intybus), and ribwort plantain (Plantago...

  6. Pflanzenbauliche Nutzung von Bioporen im Unterboden

    OpenAIRE

    Perkons, Ute; Küpper, Paul Martin; Kautz, Timo; Köpke, Ulrich

    2013-01-01

    Biopores act as pathways for the roots of following crops to access water and nutrients stored in the subsoil. Their role for nutrient management is especially important under the conditions of Organic Agriculture. In a field trial on Haplic Luvisol, chicory (Cichorium intybus L., tap root system) and tall fescue (Festuca arundinacea, fibrous root system) were grown as precrops. Effects on biopore density, root-length density (RLD), leaf area index (LAI), biomass, transpiration and photosynth...

  7. Herbs in grasslands - effect of slurry and grazing/cutting on species composition and nutritive value

    OpenAIRE

    Søegaard, Karen; Eriksen, Jørgen; Askegaard, Margrethe

    2008-01-01

    Herbs are established in many organic grasslands due to their expected beneficial properties for nutritive value and biodiversity. However, knowledge about grassland herbs is limited. Three mixtures were therefore established at different grazing/cutting management and slurry applications. The competitiveness of the species varied greatly. Plantain (Plantago lanceolata) and lucerne (Medicago sativa) competed best under cutting; in contrast, chicory (Cichorium intybus) competed best under graz...

  8. [Antiviral activity of extracts of transgenic cichory and lettuce plants with the human interferon alpha-2b gene].

    Science.gov (United States)

    Matveeva, N A; Kudriavets, Iu I; Likhova, A A; Shakhovskiĭ, A M; Bezdenezhnykh, N A; Kvasko, E Iu

    2012-01-01

    Biological activity of protein extracts from transgenic plants of chicory Cichorium intybus L. and lettuce Lactuca sativa L. with human interferon alpha2b gene was investigated against vesicular stomatitis virus. It was shown that the extracts from the hairy roots of chicory and lettuce transformed by A. rhizogenes possess the antiviral activity 1620...5400 IU/g weight, and the extracts from leaves of the plants transformed by A. tumefaciens--till 9375 IU/g weight. Dependence of plant extract biological activity on the transformation vector was shown. PMID:23342646

  9. Chicory roots improves the taste and odour of organic pork

    OpenAIRE

    Roepstorff, Allan; Mejer, Helena; Thomsen, Lisbeth E.; Thamsborg, Stig M.; Byrne, Derek V.; Hansen, Laurits L.; Bach Knudsen, Knud Erik; Møller, Kristian

    2005-01-01

    The carbohydrate inulin is known to reduce the production of skatole in pigs. This is caused by inulin changing the intestinal flora, so that the bacteria that produce skatole are held in check. This change in intestinal flora also reduces the number of intestinal parasites in the pigs. However the high cost of inulin makes its use in pig feed impractical. Chicory root contains inulin and a series of other carbohydrates and secondary metabolites. Therefore we have examined whether chicory...

  10. Hydrolase activity in Jerusalem artichoke and chicory

    Energy Technology Data Exchange (ETDEWEB)

    Klaushofer, H.; Abraham, B.; Leichtfried, G.

    1988-03-01

    Post-harvest storage of chicory and Jerusalem artichoke and overwintering of Jerusalem artichoke in the soil cause a more or less pronounced shortening of the fructan chain, depending on the variety. The proportion of fructose in the total fructan thus shifts towards glucose. This reduction on the fructose/glucose ratio is undesirable if the intention is to obtain a sweetener of high fructose content. In this work an attempt was made, via the quantity of fructose formed after a 4(3)-hour reaction of a tuber (root) extract with inulin, to assign a characteristic value to the depolymerization tendency of the material in question. However, since the plant extract not only contains enzymes (hydrolase A and B) that shorten the fructan chains but the activity of fructosyltransferase (SST, FFT) and enzymes of microbial origin (inulinase II, invertase) must also be considered, the concept of 'hydrolase activity' used by the authors is essentially an expression of 'total activity'. The activity unit (EU) is defined as the ability to split of 1 ..mu..mol of fructose from (chicory) inulin per minute under experimental conditions. Values of 0.25 to 0.77 EU/g dry solids were found in Jerusalem artichoke. Considerable differences may occur between varieties from the same cultivated area and the same harvest period. With one and the same variety, the activity appears to be subject to marked yearly fluctuations, so that at present, because of hydrolase activity, nothing certain can be said about the depolymerization tendency of a variety.

  11. Crop characteristics and inulin production in chicory

    International Nuclear Information System (INIS)

    Crop growth, dry matter partitioning, leaf area development, light interception and dry matter : radiation quotient in chicory were studied in field and glasshouse trials. Variations in root and inulin yields were related to sowing time, sowing density and cultivar. Retarded growth of first leaves appeared to be a major factor in limiting productivity. Growth of the first leaves was limited by assimilate supply and by low temperatures. Leaf area expansion exhibited a lag of 350 °Cd from emergence. From that point until crop closure, leaf area index increased exponentially with thermal time. Initially, 60 per cent of the dry matter was partitioned to the leaves; this share gradually decreased to about 10 per cent during later stages. The average dry matter: radiation quotient was 2.6 g MJ-1 for total dry matter and 2.4 g MJ-1 for root dry matter. Cultivars differed in early leaf growth, dry matter partitioning and dry matter: radiation quotient. The crop characteristics are compared with literature data for sugar beet and the prospects for breeding improved genotypes are discussed. (author)

  12. Effect of Fertilising Fodder Chicory on Fodder Quality

    OpenAIRE

    Nicoleta Moraru; Dragomir Neculai; Camen Dorin; Dragomir Carmen; Rechiţean Dorin; Sala Anca; Neciu Florin

    2015-01-01

    The nutritive value of fodder chicory is close to the quality of fodder alfalfa, i.e. under certain technological conditions, fodder chicory has a higher nutritive value and more raw protein. Studies show that over three experimental years, the content of raw protein reached values ranging between 23.69 and 25.26% with mineral fertilisation and between 21.88 and 22.81% with organic fertilisation, compared to only 21.06% in the control variant. Depending on the content of raw protein, the amou...

  13. Occupational Rhinoconjunctivitis and Asthma Caused by Chicory and Oral Allergy Syndrome Associated With Bet v 1-Related Protein

    OpenAIRE

    Pirson, F.; Pilette, Charles; Detry, Bruno

    2009-01-01

    We report the case of a patient working in a factory producing inulin from chicory who developed rhinoconjunctivitis and asthma to the dust of dry chicory roots and oral allergy syndrome to raw fruits and vegetables. Nonspecific bronchial hyperresponsiveness was diagnosed. A provocation test with dry chicory induced acute rhinoconjunctivitis and an immediate asthmatic response with no further clinical symptoms. Skin prick test results were positive to birch pollen and fresh/dry chicory, and n...

  14. ATTEMPT TO REDUCE ACRYLAMIDE CONTENT IN ROASTED CHICORY

    Directory of Open Access Journals (Sweden)

    Gabriela Zięć

    2015-02-01

    Full Text Available The aim of this study was to reduce the formation of acrylamide during roasting of chicory roots by soaking the fresh roots in a solution of calcium chloride, by the use of different temperature and time of roasting of dried roots, as well as by the addition of the enzyme (asparaginase during roasting of dried roots. It was shown, that with increasing roasting temperature of chicory roots from 100 - 175 ° C the acrylamide content also increased, while at a temperature of 210 ° C the growth was inhibited. Increasing roasting time from 10 - 25 minutes resulted in an increased acrylamide content. Soaking the roots in the CaCl2 solution for 20 minutes reduced the formation of acrylamide during the roasting approximately by 40%, similarly as the application of asparaginase to the dried roots during the roasting process.

  15. Effect of Fertilising Fodder Chicory on Fodder Quality

    Directory of Open Access Journals (Sweden)

    Nicoleta Moraru

    2015-10-01

    Full Text Available The nutritive value of fodder chicory is close to the quality of fodder alfalfa, i.e. under certain technological conditions, fodder chicory has a higher nutritive value and more raw protein. Studies show that over three experimental years, the content of raw protein reached values ranging between 23.69 and 25.26% with mineral fertilisation and between 21.88 and 22.81% with organic fertilisation, compared to only 21.06% in the control variant. Depending on the content of raw protein, the amount of raw protein increased with 40.7% with mineral fertilisation and with 22.4% with organic fertilisation, compared to the control variant.

  16. Effects of catch crop type and root depth on nitrogen leaching and yield of spring barley

    DEFF Research Database (Denmark)

    Sapkota, Tek Bahadur; Askegaard, Margrethe; Lægdsmand, Mette;

    2012-01-01

    [chicory (Cichorium intybus L.), fodder radish (Raphanus sativus L.) and perennial ryegrass (Lolium perenne L.)] and their effect on soil mineral N (NO3− and NH4+) in different soil layers by using the FASSET model. The simulated results of catch crop biomass and root growth and mineral N in the soil...... simulations showed that fodder radish developed the deepest root system and depleted N from deeper soil layers than chicory and ryegrass. Thirty years of simulations showed that the system with ryegrass catch crop had a smaller amount of N leaching from 1 m depth than the system with other catch crops and...... without catch crops. However, estimated total N leached at 2 m soil depth was smallest in the system with fodder radish followed by the system with chicory, indicating that these catch crops are capable of taking soil N also from below 1 m depth. On average, the system with fodder radish was estimated to...

  17. In vivo efficacy of chicory silage against parasitic nematodes in cattle

    DEFF Research Database (Denmark)

    Pena-Espinoza, Miguel Angel; Desrues, Oliver; Williams, A.;

    . Spadona) was ensiled and fermented for 5-12 weeks. 15 Jersey male calves (2-4 months) were stratified by live weight and randomly allocated to 2 groups: chicory (CHI, n=9) and control (CON, n=6). CHI and CON calves were fed with chicory silage and hay ad libitum, resp., for 8 weeks. Daily intake was...

  18. Flowering induction in radicchio (cichorium intybus l.var silvestre bischoff:the influence of vernalization in different types and classes of precocity

    Directory of Open Access Journals (Sweden)

    Carlo Nicoletto

    2011-02-01

    Full Text Available All experiments were carried out at the Experimental Station “L. Toniolo” of the University of Padova (45°21’ N; 11°58’ E. Experiment 1. This research was undertaken to study the effect of increasing duration of cold periods (0-5-10-15- 20 and 30 days on three classes of “Rosso di Chioggia” with different precocity: “Perseo” (Precocious, “Leo” (Medium and “Virgo” (Late. Seeds were sown on different dates (V30-V20-V15-V10-V5 and V0, i.e. May 10th- 20th-25th and 30th, June 5th and 10th 2004 respectively and placed in pre-formed polystyrene multiple trays. Treatments with low temperatures (5 °C started on the 1st June for V30 and for the others (V5-V10-V15 and V20 25-20-15 and 10 days later in controlled growth chambers, while plants that did not require vernalization stayed in the greenhouse. After vernalization and its stabilisation for 15 days, plants were transplanted in open field (July 24th. Moreover, on the 30th of August the number of leaves in rosette and elongated stem on five flowered plants for each group was determined, to verify the possible existing relation between flowering and the number of leaves on plant. Experiment 2. This experiment was undertaken to compare the effect of vernalization on flowering of 8 cultivars of radicchio to assess their membership of annual or biennial species. The 8 cultivars were Rosso di Treviso tardivo “Sile tardivo”, Rosso di Treviso precoce “Mesola precoce”, Rosso di Chioggia “Perseo” (P, “Leo” (M and “Virgo” (L, Rosso di Verona “Cologna medio”, Variegato di Lusia “Adige tardivo” e Variegato di Castelfranco “Castellano medio”. All types were vernalized for 0-5 and 15 days (V0-V5 and V15. Seeds were placed (January 15th-25th and 30th 2005 for V15, V5 and V0 respectively in pre-formed polystyrene multiple trays. Vernalization (5 °C started on February 14th in controlled growth chambers and, at the end, was stabilized for 15 days. After vernalization and its stabilisation, plants were transplanted in open field (May 26th. In both experiments, after the transplant, plants were controlled everyday to determine the exact day in which the stem elongation started (stage 51 BBCH. Results showed that: (1 there is no relation between the number of leaves on plant and flowering; (2 vernalization exerts different effect on promoting flowering among types; (3 on average the effect of cold period is quantitatively related to its duration; (4 low temperature increased flowering precocity anticipating the stage of phase transition; (5 the behaviour of different types of radicchio shows that Rosso di Treviso tardivo is probably a biennial plant, while the others act as annual species.

  19. Bioactive β-carbolines norharman and harman in traditional and novel raw materials for chicory coffee.

    Science.gov (United States)

    Wojtowicz, Elżbieta; Zawirska-Wojtasiak, Renata; Przygoński, Krzysztof; Mildner-Szkudlarz, Sylwia

    2015-05-15

    The β-carboline compounds norharman and harman exhibit neuroactive activity in the human body. Chicory coffee has proved to be a source of β-carboline compounds. This study assessed the norharman and harman contents of traditional and novel raw materials for the production of chicory coffee, as well as in samples of chicory coffee with novel additives. The highest content of the β-carbolines among the traditional raw materials was recorded in roasted sugar beet (2.26 μg/g), while roasting the chicory caused a 25-fold increase in the content of norharman in this raw material (from 0.05 to 1.25 μg/g). In novel raw materials not subjected to the action of high temperature, β-carboline was not detected. Among the roasted novel raw materials, the highest contents of harman and norharman were found in artichokes. High harman levels were also recorded in roasted chokeberry. PMID:25577081

  20. Hepatoprotective effect of feeding celery leaves mixed with chicory leaves and barley grains to hypercholesterolemic rats

    OpenAIRE

    Abd El-Mageed, Nehal M.

    2011-01-01

    Celery, chicory leaves, and barley grains are valuable in weight loss diets and regulate lipid metabolism. They may reduce risk of fatty liver. The present study aimed to investigate the effect of diet supplementation with celery, chicory, and barley powder on liver enzymes and blood lipids in rats fed with cholesterol-enriched diet. This study used four groups of rats fed with 3% cholesterol were supplemented diet to induce hypercholesterolemia and one group was fed on cholesterol-free basal...

  1. Feeding with chicory roots reduces the amount of odorous compounds in colon contents of pigs

    OpenAIRE

    Hansen, L L; Jensen, M.T.

    2005-01-01

    It is known that pure inulin a fructooligosaccharide extracted from chicory roots can: reduce boar taint (skatole in backfat and blood)reduce parasite infection levels when added to specially composed experimental diets. However, the entire chicory roots may, in comparison to inulin: reduce boar taint more effectively improve the taste of cooked meat from both male and female pigs be more effective against parasites when added to normal diet types Improved stable environment due...

  2. Production of PHB from Chicory Roots - Comparison of Three Cupriavidus necator Strains

    OpenAIRE

    Haas, C.; Steinwandter, V.; Diaz De Apodaca, E.; Maestro Madurga, B.; Smerilli, M.; Dietrich, T.; Neureiter, M.

    2015-01-01

    Chicory roots from hydroponic salad cultivation are an abundant food residue in Navarra (Spain) that are underutilized to date. Aiming at a holistic utilization of resources, we report here the first process using chicory root hydrolysate for the production of poly([R]-3-hydroxybutyrate) (PHB). The polymer can be used for packaging material made for the locally produced vegetables. In the first step, we developed a pre-treatment process to obtain a hydrolysate, which contained 34 g L-1 s...

  3. Productivity and quality, competition and facilitation of chicory in ryegrass/legume-based pastures under various nitrogen supply levels

    OpenAIRE

    Høgh-Jensen, Henning; Nielsen, Bea; Thamsborg, Stig Milan

    2006-01-01

    Traditional perennial ryegrass-white clover mixtures have limitations in combined productivity and quality that herbs like chicory may alleviate. This study examined the consequences on productivity and quality of as well as competition and facilitation after introducing chicory into varies ryegrass-legume-based pastures in a field study over 3 consecutive growing seasons. A cultivar of chicory, suitable for grazing, in pure stand was found to out-yield a pure stand ryegrass in terms of dry m...

  4. Study of the incidence of intestinal parasites in vegetables commercializes in free trade fair and supermarket Londrina

    Directory of Open Access Journals (Sweden)

    Priscila Ruzzon Nomura

    2015-03-01

    Full Text Available The objective of this study was to determine the presence of intestinal parasites in samples of lettuce and chicory sold in street market and supermarket Londrina city, Paraná. For this purpose, eight samples of lettuce (Lactuca sativa and eight samples of chicory (Cichorium intybus were collected and analyzed. The analysis was performed at the Laboratory for Extension and Research in Enteroparasitosis – L.E.P.En. Cysts of Endolimax nana, Entamoeba coli, Entamoeba histolytica, Balantidium coli and Strongyloides stercoralis larvae, Hookworm larvae and eggs, and eggs of Ascaris spp were found in the samples. These results demonstrate the need for hygienic-sanitary measures, aimed at educating the community, enabling the improvement in living conditions of the population.

  5. Lipid modulatory activities of Cichorium glandulosum Boiss et Huet are mediated by multiple components within hepatocytes

    OpenAIRE

    Lin Ding; Jun-Lin Liu; Waseem Hassan; Lu-Lu Wang; Fang-Rong Yan; Jing Shang

    2014-01-01

    To investigate a possible methodology of exploiting herbal medicine and design polytherapy for the treatment of non-alcoholic fatty liver disease (NAFLD), we have made use of Cichorium glandulosum Boiss et Huet (CG), a traditional Chinese herbal medicine that has been proven to be effective in treating hepatic diseases. Here, we report that the extract of CG effectively reduced lipid accumulation under conditions of lipid overloading in vivo and in vitro (in a rat high-fat diet model and a he...

  6. Quantitative determination of hydroxycinnamic acids in chicory root

    OpenAIRE

    Yezerska, Oksana; Kalynyuk, Tymofiy; Vronska, Lyudmula

    2013-01-01

    The possibility for applying of a direct spectrophotometry to quantify totality of hydroxycinnamic acid derivatives is investigated. As a quantitative criterion for quality assessment of chicory roots the total content of hydroxycinnamic acid derivatives of at least 0.3 %, expressed as chlorogenic acid, is suggested. Досліджено можливість застосування прямої спектрофотометрії для кількісного визначення гідроксикоричних кислот. Як кількісний критерій якості коренів цикорію запропоновано вміст...

  7. Influence of Nitrogen Fertilization Upon the Concentration of Nitrates in Head Chicory

    Directory of Open Access Journals (Sweden)

    Mirjana Ćustić

    1997-12-01

    It is noteworthy for these investigations that, regardless of fertilization and ivestigation year, all the determined nitrate values of harvested chicory (555 - 1057 mg N03-/kg F. W. were below tolerance according to international standards for this type of leafy vegetables (1500 - 2500 mg N03-/kg F. W.

  8. Comparative Study on Regeneration and Genetic Transformation between Puna Chicory and Commander Chicory%2种菊苣再生体系及遗传转化效率的比较

    Institute of Scientific and Technical Information of China (English)

    赵龙; 陈丹丹; 梁明祥; 郑青松; 王长海; 刘兆普

    2012-01-01

    Effects of culture medium composition on callus induction, shoot regeneration and root formation from cotyledon explants in two kinds of chicory(Puna chicory and Commander chicory) were evaluated to optimize the plant regeneration system. The Aeluropus littoralis Na+/H+ antiporter (AINHX) gene, which encodes a Na+/H+ antiporter, was introduced into chicory to evaluate their genetic transformation efficiency. The results showed that the callus induction and shoot regeneration varied from chicory genotypes. The optimum medium for Puna chicory and Commander chicory were MS+1. 5 mg/L 6-BA + 0. 2 mg/L IBA and MS+1. 0 mg/L 6-BA + 0. 5 mg/L NAA,respectively. The optimum medium for Puna and Commander chicory root formation was 1/2MS+0. 1 mg/L NAA. The insertion of AINHX gene into chicory genome was confirmed by PCR. The genetic transformation efficiency of Puna chicory and Commander chicory were 10. 0% and 13. 3% respectively.%以普那菊苣和将军菊苣子叶为材料,通过植物组织培养的方法,探讨了不同激素浓度配比对二者愈伤组织诱导、芽分化以及根再生的影响,并通过农杆菌介导法将编码獐茅液泡膜Na+/H+逆向转运蛋白基因(AlNHX)导入菊苣中,比较普那菊苣和将军菊苣的遗传转化效率.结果表明:不同基因型的菊苣愈伤组织诱导和芽分化条件不同,普那菊苣最佳培养基为MS+1.5 mg/L6-BA+0.2 mg/L IBA;将军菊苣最佳培养基为MS+ 1.0 mg/L 6-BA+0.5 mg/L NAA;二者最佳生根培养基均为1/2MS+0.1mg/L NAA.获得的抗性芽经PCR检测,初步证实AINHX已插入到菊苣基因组中,且普那菊苣转化效率为10.0%,将军菊苣转化效率为13.3%.

  9. HEALTHY PROPERTIES OF ENDIVE (CICHORIUM ENDIVIA L.) DEPENDING ON THE VARIETY AND VEGETATIVE OF SEASON

    OpenAIRE

    Iwona Mentel; Ewa Cieślik; Anna Sadowska-Rociek

    2015-01-01

    The endive (Cichorium endivia L.) is plant belonging to Asteraceae family. It is very popular vegetable in France, Italy and Germany. Endive because of its high content healthy components can be used in prevention against a lot of diseases. The studies were conducted on two varieties of endive: ‘Cigal’ and ‘Marconi’ during vegetative of season: 2009 and 2010. The research material was assessed for: dry matter, protein, dietary fibre, ash, selected minerals (Ca, Mg, K, P, Na, Fe, Zn, Mn, C...

  10. The effect of a diet with fructan-rich chicory roots on intestinal helminths and microbiota with special focus on Bifidobacteria and Campylobacter in piglets around weaning

    DEFF Research Database (Denmark)

    Jensen, Annette Nygaard; Mejer, Helene; Mølbak, Lars;

    2011-01-01

    The restrictions on the use of antibiotic and anthelmintic treatments in organic pig farming necessitate alternative non-medical control strategies. Therefore, the antibiotic and parasite-reducing effect of a fructan-rich (prebiotic) diet of dried chicory was investigated in free-ranging piglets...... either chicory (30% dry matter) or a control diet. The effect of chicory on intestinal helminths, intestinal microbiota, especially Bifidobacteria and Campylobacter spp. and E. coli post-weaning diarrhoea was assessed. The weight gain of the piglets was not impaired significantly by chicory. The...... intestinal A. suum worm burden was reduced by 64% (P = 0.034) in the chicory-fed piglets, whereas these same piglets had 63% more T. suis worms (P = 0.016). Feeding with chicory elicited no changes among the main bacterial groups in ileum according to terminal restriction fragment length polymorphism...

  11. 优质牧草菊苣栽培技术%Cultivation Techniques of Chicory

    Institute of Scientific and Technical Information of China (English)

    徐顺乾

    2009-01-01

    菊苣(cichioriam intybus L)为菊科,菊苣属多年生主根型草本植物,原产于欧洲,20世纪70年代末引入我国,具有适口性好、抗病力强、易于栽培、产草量高等优点。

  12. A Method for the Simultaneous Determination of Chlorogenic Acid and Sesquiterpene Lactone Content in Industrial Chicory Root Foodstuffs

    OpenAIRE

    Honorine Willeman; Philippe Hance; Anne Fertin; Najia Voedts; Nathalie Duhal; Jean-François Goossens; Jean-Louis Hilbert

    2014-01-01

    A method for the simultaneous determination of free chlorogenic acids (CGA) and sesquiterpene lactones (STL) in chicory root and its dried (flour) and roasted (grain) forms is described. The method uses one extraction and one analysis for all chicory root products. Various solvents with low to high polarity, such as methanol, chloroform, or n-hexane, were tested alone, in combination in different proportions or with acidified or neutral aqueous solvent. The water/chloroform/methanol (30/30/40...

  13. Feeding driet chicory root to pigs decrease androstenone accumulation in fat by increasing hepatic 3β hydroxysteroid dehydrogenase expression

    OpenAIRE

    Rasmussen, Martin Krøyer; Brunius, Carl; Zamaratskaia, Galia; Ekstrand, Bo

    2012-01-01

    The present study investigated the in vivo effect of chicory root on testicular steroid concentrations and androstenone metabolizing enzymes in entire male pigs. Furthermore, the effect on skatole and indole concentrations in plasma and adipose tissue was investigated. The pigs were divided into two groups; one receiving experimental feed containing 10% dried chicory root for 16 days before slaughter, the control group was fed a standard diet. Plasma, adipose and liver tissue samples were col...

  14. Dehydrated chicory pulp as an alternative soluble fibre source in diets for growing rabbits

    Directory of Open Access Journals (Sweden)

    L. Maertens

    2014-06-01

    Full Text Available Soluble fibre (SF is an important nutrient to enhance fermentative activity and gut health in rabbits. The main source of SF in rabbit diets is sugar beet pulp (SBP, whereas, due to its high content of SF (34%, dried chicory pulp (ChP could be an alternative to SBP. In a fattening trial with 192 hybrid weanlings 32 d old weighing 837±45 g, chicory pulp was used in replacement of SBP to study effects on production performances and slaughter characteristics. Rabbits were fed one of 4 iso-energetic (9.65 MJ digestible energy/kg and isonitrogenous (15.6% crude protein diets: a negative control (NC diet with a low dietary SF content (7.3%, a positive control diet with quite a high SBP level (13.5% and SF content (10.6% and 2 diets with respectively 10% and 20% of chicory pulp (ChP10: 9.9% SF and ChP20: 13.7% SF. The SF content was measured as the difference between total dietary fibre and neutral detergent fibre, the latter corrected for ash and protein content. Each dietary treatment consisted of 12 replicates of 4 rabbits. Weight gain was high (on av. 54 g/d and comparable for the NC, SBP and ChP diets. However, feed conversion ratio was improved (P<0.05 with the ChP20 diet compared to the NC diet (2.88 vs. 2.97. Mortality was low and not influenced by the dietary treatment. Slaughter data were very similar and no effect of the SF level on caecal weight or slaughter yield was observed. It was concluded that chicory pulp is a good alternative soluble fibre source in balanced diets for rabbits and can be used at least up to 20% inclusion rate.

  15. Effects of different shelf storage conditions of chicory on the colour change using digital image processing

    OpenAIRE

    SCHREVENS, E.; De Busscher, R; DE BAERDEMAEKER, J; Zhang, M.

    1996-01-01

    The study established a method of evaluating the colour change of chicory under different shelf storage conditions. The RGB system was used for digitising the colour images. The experiments under four storage temperatures and three humidities were made. In the experiments, a comprehensive index, Scy was presented and used for estimating the unfavorable colour change (turning to brown and red). The index is based on comprehensive consideration of the coloured area and degree of the colour chan...

  16. Reasoned opinion on the modification of the existing MRL for pyraclostrobin in chicory roots

    Directory of Open Access Journals (Sweden)

    European Food Safety Authority

    2014-05-01

    Full Text Available In accordance with Article 6 of Regulation (EC No 396/2005, Belgium, hereafter referred to as the evaluating Member State (EMS, compiled an application to modify the existing MRL for the active substance pyraclostrobin in chicory roots. In order to accommodate for the intended use of pyraclostrobin, Belgium proposed to raise the existing MRL from the limit of quantification (LOQ of 0.02* mg/kg to 0.09 mg/kg. Belgium drafted an evaluation report in accordance with Article 8 of Regulation (EC No 396/2005, which was submitted to the European Commission and forwarded to EFSA. According to EFSA the data are sufficient to derive a MRL proposal of 0.08 mg/kg for the intended use on chicory roots. Adequate analytical enforcement methods are available to control the residues of pyraclostrobin on the commodity under consideration. Based on the risk assessment results, EFSA concludes that the proposed use of pyraclostrobin on chicory roots will not result in a consumer exposure exceeding the toxicological reference value and therefore is unlikely to pose a consumer health risk.

  17. Interactive Effects of Sowing Date and Planting Density on Dry Matter Accumulation and Partitioning of Chicory

    Directory of Open Access Journals (Sweden)

    Hamid MADANI

    2012-05-01

    Full Text Available Chicory is considered one of the alternatives crops that can be used in crop rotation and contains many phytochemicals that can be used in medicine. In addition, lengthening the growing season by early sowing may increase root chicory yield potential, and thus increase its competitiveness with traditional crops. The objectives of the present study were to determine whether early sowing date risks can be decreased by higher sowing density and also to study the effect of sowing date and sowing density on dry matter accumulation and partitioning of chicory. Growing season did not affect any of the characteristics that were studied. Also plant density affected the flowers biomass, root biomass per plant and the respective yield together with the plant height and essence yield and total yield. The sowing date affected the leaf, flower and stem biomass on a plant basis. However, the interaction between plant density and sowing date affected the total biomass per plant, the flower biomass per plant, the root biomass per plant, the flower yield, the root yield and the essence yield. These results indicate that for higher production it is important to determine the right plant density and sowing date which can affect growth, dry matter accumulation and essence yield.

  18. Cardioprotective effect of aqueous extract of Chichorium intybus L. on ischemia-reperfusion injury in isolated rat heart

    Directory of Open Access Journals (Sweden)

    Najmeh Sadeghi

    2015-10-01

    Full Text Available Objective: Several studies have shown that Chichorium intybus L. (C. intybus which possesses flavonoid compounds has an effective role in treatment of cardiovascular diseases. Contractile dysfunction mostly occurs after acute myocardial infarction, cardiac bypass surgery, heart transplantation and coronary angioplasty. The aim of the present study was to investigate the effect of aqueous extract of C. intybus on ischemia- reperfusion injury in isolated rat heart. Materials and Methods: The animals were divided into four groups (Sham, Control, 1 mg/ml and 3 mg/ml of extract of 8 rats. The aorta was cannulated, and then the heart was mounted on a Langendorff apparatus. Next, a balloon was inserted into the left ventricle (LV and peak positive value of time derivate of LV pressure (+dp/dt, coronary flow (CF, and left ventricular systolic pressure (LVSP in pre-ischemia and reperfusion period were calculated by a Power Lab system. All groups underwent a 30-minute global ischemia followed by a 60-minute reperfusion. Results: The results showed that heart rate (HR, coronary flow, and left ventricular developed pressure (LVDP and rate of pressure product (RPP significantly decreased in the control group during reperfusion, while these values in the groups receiving the extract (3mg/ml improved significantly during reperfusion (p

  19. The use of legume and herb forage species to create high performance pastures for sheep and cattle grazing systems Uso de leguminosas e forrageiras herbáceas para criar pastos de alto desempenho para sistema de pastejo de ovinos e bovinos

    Directory of Open Access Journals (Sweden)

    Peter David Kemp

    2010-07-01

    Full Text Available Sheep and cattle farmers need pastoral systems that are more productive and environmentally sustainable. The role that high feeding value herb and legume forage species can play in the farms of the future is highlighted. It is shown that species such as chicory (Cichorium intybus, plantain (Plantago lanceolata, red clover (Trifolium pratense and white clover (T.repens can provide live weight gains in lambs that are 70% greater than those from perennial ryegrass (Lolium perenne based pastures. A case for replicated experiments that examine the value of whole technology packages based on herb and legume species, rather than component research, is made.Produtores de ovinos e bovinos necessitam de sistemas pastoris mais produtivos e ambientalmente sustentáveis. As espécies forrageiras herbáceas e leguminosas de alto valor nutricional podem ter um papel destacado no futuro desses produtores. Foi demonstrado que espécies tais como a chicória (Cichorium intybus, plantago (Plantago lanceolata, trevo vermelho (Trifolium pratense e trevo-branco (T. repens podem fornecer um ganho de peso 70% maior em carneiros quando comparado com pastagens de azevém (Lolium perenne. Mais que um componente de pesquisa, este é um caso para repetidos experimentos que examinem o valor de todos os pacotes tecnológicos baseados em espécies herbáceas e leguminosas.

  20. Direct conversion of chicory flour into L(+)-lactic acid by the highly effective inulinase producer Lactobacillus paracasei DSM 23505.

    Science.gov (United States)

    Petrova, Penka; Velikova, Petya; Popova, Luiza; Petrov, Kaloyan

    2015-06-01

    Highly effective bio-process for lactic acid (LA) production by simultaneous saccharification and fermentation (SSF) of chicory flour was developed. The strain used, Lactobacillus paracasei DSM 23505 produced natural inulinase (EC 3.2.1.80) with molecular weight ∼130 kDa, located in the cell wall fraction. In batch fermentation with optimized medium content and fermentation conditions, a complete conversion of 136 g/L chicory flour (89.3% inulin and 10.7% mix of sucrose, fructose and glucose) into 123.7 g/L LA was achieved. These yield and conversion rate are the highest obtained by SSF for LA production from inulin. The high efficiency, the cheap fermentation broth and the simple process performance disclose the promising use of the chicory flour in industrial biotechnology for LA production. PMID:25824595

  1. Effects of including chicory in perennial ryegrass-white clover leys on production and health in organic lambs

    OpenAIRE

    Nielsen, Bea; Thamsborg, Stig Milan; Hansen, Hanne; Ranvig, Hans; Høgh-Jensen, Henning

    2009-01-01

    Two trials were conducted to examine the effect of chicory included in or as additional plots of the ryegrass–white clover pastures on growth, naturally acquired nematode infections and selectivity of chicory by lambs. In Experiment 1, 31 weaned Texel lambs (36±7 kg) grazed either ryegrass–white clover (CTRL) or ryegrass–white clover–hicory pastures (CHIC). In Experiment 2, 30 weaned Shropshire lambs (32±7 kg) grazed either ryegrass–white clover (CTRL) or ryegrass–white clover with access to...

  2. Loss of function of 1-FEH IIb has more impact on post-harvest inulin degradation in Cichorium intybus than copy number variation of its close paralog 1-FEH IIa

    OpenAIRE

    Dauchot, Nicolas; Raulier, Pierre; Maudoux, Olivier; Notté, Christine; Draye, Xavier; Van Cutsem, Pierre

    2015-01-01

    Key Message: The loss of mini-exon 2 in the 1-FEH IIb glycosyl-hydrolase results in a putative non-functional allele. This loss of function has a strong impact on the susceptibility to post-harvest inulin depolymerization. Significant variation of copy number was identified in its close paralog 1-FEH IIa, but no quantitative effect of copy number on carbohydrates-related phenotypes was detected. Inulin polyfructan is the second most abundant storage carbohydrate in flowering plants. After ...

  3. Hindiba (Cichorium intybus L.) Bitkisinden Myrosinaz Enziminin Saflaştırılması, Karakterize Edilmesi ve Kozmetik Alanında Kullanılabilirliğinin İncelenmesi

    OpenAIRE

    DEMİR, Nazan; Esen TAŞGIN

    2014-01-01

    Myrosinaz glukosinolatların hidrolizinden sorumlu bir enzimdir (E.C: 3.2.3.2 β-thioglucosidase, β-thioglucoside glucohydrolase olarak da bilinir). Kanserle mücadele için umut verici bileşikler olarak kabul edilen sinigrin, glukoraphanin (GRA) ve glukoraphenin (GRE) gibi ara yıkım ürünlerinden dolayı bazı alifatik glukosinolatlar oldukça ilgi çekmiş ve myrosinaz enziminin bu tümör küçültücü etkisi son yıllarda önem kazanmıştır. Bu çalışmada, myrosinaz amonyum sülfat çöktürmesi, Q...

  4. Effects of Aqueous Extracts of Chicory and Milk Thistle on Serum Concentrations of Copper, Zinc, and Manganese in Tamoxifen-Treated Rats.

    Science.gov (United States)

    Abbasalipourkabir, Roghayeh; Ziamajidi, Nasrin; Nasiri, Abolfazl; Behrouj, Hamid

    2016-09-01

    Some medications may change trace element levels in the body. Extracts of various plants, due to having the several elements, can have beneficial effects. Consumption of herbal extracts with chemical drugs may reduce adverse effects of medication. The goal of this study was to evaluate copper (Cu), zinc (Zn), and manganese (Mn) concentrations in serum of rats treated with tamoxifen, chicory, and/or milk thistle extracts. Therefore, 36 adult female Wistar rats were divided into six groups: normal control, chicory control, milk thistle control, tamoxifen, tamoxifen-chicory, and tamoxifen-milk thistle. At the end of the study, the blood samples were collected and sera isolated by centrifugation and analyzed by the atomic absorption spectrophotometry for Cu, Zn, and Mn levels. The Zn concentration increased in milk thistle-supplemented groups. The Cu level increased in the chicory control group only. Tamoxifen had no affect on Cu, Zn, and Mn levels, but seed extract of milk thistle increased Zn concentration, and chicory root extract increased Cu concentration. Although elevated levels of Cu in rats receiving tamoxifen-chicory were milder than rats treated only with chicory, it seems that the extract and tamoxifen impact on the Cu are in conflict with each other. PMID:26875177

  5. Hepatoprotective effects of parsley, basil, and chicory aqueous extracts against dexamethasone-induced in experimental rats

    Directory of Open Access Journals (Sweden)

    Hanan A. Soliman

    2016-03-01

    Full Text Available Aim:The objective of this study is to investigate the hypoglycemic, hypolipidemic, and hepatoprotective effects of the aqueous extract of parsley, basil, and chicory whole plant in normal and dexamethasone (Dex rats. Materials and Methods: 50 female albino rats were used in this study and divided into 5 groups (for each 10. Group (1 fed basal diet and maintained as negative control group. Group (2 received Dex in a dose of (0.1 mg/kg b. wt.. Groups 3, 4, and 5 were treated with Dex along with three different plant extracts of parsley, basil, and chicory (2 g/kg b. wt., (400 mg/kg b. wt., and (100 mg/kg b. wt., respectively. Results: All these groups were treated given three times per week for 8 consecutive weeks. Dex-induced alterations in the levels of serum glucose, triglyceride, cholesterol, low-density lipoprotein-cholesterol levels and cardiovascular indices and serum alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase activities, liver thiobarbituric acid (TBARS levels increased, while high-density lipoprotein-cholesterol, total protein, albumin, and liver glutathione (GSH levels decreased. On the other hand, plant extracts succeeded to modulate these observed abnormalities resulting from Dex as indicated by the reduction of glucose, cholesterol, TBARS, and the pronounced improvement of the investigated biochemical and antioxidant parameters. Conclusions: It was concluded that probably, due to its antioxidant property, parsley, basil, and chicory extracts have hepatoprotective effects in Dex-induced in rats. [J Intercult Ethnopharmacol 2016; 5(1.000: 65-71

  6. Hepatoprotective effects of parsley, basil, and chicory aqueous extracts against dexamethasone-induced in experimental rats

    OpenAIRE

    Soliman, Hanan A.; El-Desouky, Mohamed A.; Hozayen, Walaa G.; Ahmed, Rasha R.; Khaliefa, Amal K.

    2016-01-01

    Aim:The objective of this study is to investigate the hypoglycemic, hypolipidemic, and hepatoprotective effects of the aqueous extract of parsley, basil, and chicory whole plant in normal and dexamethasone (Dex) rats. Materials and Methods: 50 female albino rats were used in this study and divided into 5 groups (for each 10). Group (1) fed basal diet and maintained as negative control group. Group (2) received Dex in a dose of (0.1 mg/kg b. wt.). Groups 3, 4, and 5 were treated with Dex along...

  7. Interactive effects of nitrogen and pH on four physiological indicators of chicory%氮与pH互作对菊苣4项生理指标的影响

    Institute of Scientific and Technical Information of China (English)

    解宝; 陈果; 杜金鸿; 王佺珍; 吴春会

    2012-01-01

    采用65.305,130.610和261.220mg.L-1三个质量浓度氮的改良Hoagland营养液及5.5、7.0和8.5三个pH水平完全随机等重复沙培试验,探讨了氮与pH互作对菊苣(Cichorium intybus)叶片叶绿素和丙二醛含量(MDA)及过氧化物酶(POD)和超氧化物歧化酶(SOD)活性等生理指标的影响。结果表明,1)在不同氮浓度处理时,菊苣叶片叶绿素和MDA含量无显著变化,而POD和SOD活性显著上升;2)在不同pH处理时,菊苣叶片叶绿素含量有显著变化,MDA含量无显著变化,而POD和SOD活性显著上升;3)氮与pH互作对菊苣生长有显著影响;菊苣叶片叶绿素含量在高氮低pH互作下最高,且随施氮量的增加持续增加;MDA含量在高氮低pH互作下最低,且在氮或pH胁迫时变化甚微;POD和SOD活性在高氮高pH互作下活性最低,且在受到氮或pH胁迫时变化显著。%A experiment was carried out to determine the interactive effects of nitrogen and pH with on chlorophyll and malondialdehyde(MDA) content,peroxidas(POD) and superoxide dismutase(SOD) activity of chicory(Cichorium intybus) by completely random combination of three nitrogen concentrations(65.305 mg·L-1,130.610 mg·L-1 and 261.122 mg·L-1) and three pH levels(5.5,7.0 and 8.5) in the sand culture.The results of this study showed the application of nitrogen did not affect the chlorophyll and MDA content but significantly affected the POD and SOD activity.The pH value significantly affected the chlorophyll but did not affected the MDA content,and increased the activity of POD and SOD.the interaction of nitrogen and pH had significant effect on chicory growth,indicating that the chlorophyll content were greatest with 65.305 mg·L-1 N and pH 5.5,and it increased as N concentration increased;the MDA content were lowest with 261.122 mg·L-1 N and pH 5.5,and it kept stable when only N or pH stress was applied;the POD and SOD activity were highest with 261.122 mg·L-1

  8. Fatty Acids, α-Tocopherol, β-Carotene and Lutein Contents in Forage Legumes, Forbs and a Grass-Clover Mixture

    DEFF Research Database (Denmark)

    Elgersma, A.; Søegaard, Karen; Jensen, Søren Krogh

    2013-01-01

    fatty acids (FA) in a variety of forage legumes and non-legume forb species compared to a grass-clover mixture and to explore implications for animal-derived products. Seven dicotyledons [four forbs (salad burnet ( Sanguisorba minor ), caraway ( Carum carvi ), chicory ( Cichorium intybus ), and ribwort...... 2009 and 2010. The experimental design was a randomized complete block, and analyses of variance were performed. In addition, three other forbs were grown: borage ( Borago officinalis ), viper's bugloss ( Echium vulgare ), and chervil ( Anthriscus cerefolium ). Lucerne and yellow sweet clover had the...... (33.7-35.7 g 100 g(-1) FA) proportions of n-3 FA. This study demonstrated higher vitamin concentrations in some forbs compared with major forages such as lucerne and grass-clover, more total FA in salad burnet, caraway, and birdsfoot trefoil than in lucerne, and higher n-3 FA concentrations in all...

  9. Effect of summer forage species grazed during finishing on animal performance, carcass quality, and meat quality.

    Science.gov (United States)

    Schmidt, J R; Miller, M C; Andrae, J G; Ellis, S E; Duckett, S K

    2013-09-01

    Angus-cross steers (n = 60) were used to assess the effect of forage species [alfalfa (AL; Medicago sativa L.), bermudagrass (BG; Cynodon dactylon), chicory (CH; Cichorium intybus L.), cowpea (CO; Vigna unguiculata L.), and pearl millet (PM; Pennisetum glaucum (L. R Br.)] in replicated 2-ha paddocks for finishing on cattle performance, carcass quality, and meat quality in a 2-yr study. Steers were blocked by BW and assigned randomly to finishing-forage treatments before the start of the experiment. Steers grazing AL and CH had greater (P 1 kg/d). Finishing on legumes (AL and CO) increased dressing percentage, reduced Warner-Bratzler shear force values, and increased consumers preference, whereas finishing on grasses (BG and PM) enhanced anticarcinogenic fatty acid concentrations. PMID:23825343

  10. Radioprotective effect of chicory seeds against genotoxicity induced by ionizing radiation in human normal lymphocytes.

    Science.gov (United States)

    Hosseinimehr, S J; Ghaffari-Rad, V; Rostamnezhad, M; Ghasemi, A; Allahverdi Pourfallah, T; Shahani, S

    2015-01-01

    The search for less-toxic radioprotective agents has led to a growing trend towards natural products. Protective effect of the methanolic extract of chicory seeds (MCS) was investigated against genotoxicity induced by ionizing radiation in human lymphocytes. Human peripheral blood samples were collected and incubated with MCS at different concentrations (10, 50, 100, and 200 μg/mL) for two hours. The whole blood samples were exposed in vitro to X-ray at dose 2.5 Gy. Then, the lymphocytes were cultured with mitogenic stimulation to determine the micronucleus in cytokinesis blocked binucleated cell. The methanolic extract at all doses significantly reduced the frequency of micronuclei in binucleated lymphocytes, as compared with similarly irradiated lymphocytes without any extract treatment. The maximum protection was observed at 200 μg/mL of MCS, it completely protected genotoxicity induced by ionizing radiation in human lymphocytes. The extract exhibited a concentration-dependent radical scavenging activity on 1,1-diphenyl-2-picryl hydrazyl free radicals. HPLC analysis of MCS showed this extract is containing chlorogenic acid as a phenolic compound. These data suggest that the radioprotective effect of methanolic extract of chicory seeds can be attributed to the presence of phenolic compounds such as chlorogenic acid which act as antioxidant agents. PMID:26278267

  11. Salinity effect on nutritional value, chemical composition and bioactive compounds content of Cichorium spinosum L.

    Science.gov (United States)

    Petropoulos, Spyridon A; Levizou, Efi; Ntatsi, Georgia; Fernandes, Ângela; Petrotos, Konstantinos; Akoumianakis, Konstantinos; Barros, Lillian; Ferreira, Isabel C F R

    2017-01-01

    Soil salinization is an increasing problem for many areas throughout the world that renders prohibitive vegetables and crop production in general. In the present study, Cichorium spinosum L. plants were grown under saline conditions in order to evaluate chemical composition and bioactive compounds content of their leaves. Salinity increase resulted in significant changes of macro and micro-nutrients content (nutritional value, sugars, fatty acids, minerals, ascorbic acid and tocopherols), whereas the concentration of phenolic compounds was not significantly affected. Chicoric and 5-O-caffeoylquinic acid were the most abundant phenolic acids. In contrast, antioxidant activity and mineral composition were beneficially affected by mid-to-high and high salinity levels. In conclusion, C. spinosum can be cultivated under saline conditions without compromising the quality of the final product, especially in semi-arid areas where irrigation water is scarce and/or of low quality due to high content of NaCl (coastal areas or areas where underground water is saline). PMID:27507457

  12. Lipid modulatory activities of Cichorium glandulosum Boiss et Huet are mediated by multiple components within hepatocytes.

    Science.gov (United States)

    Ding, Lin; Liu, Jun-Lin; Hassan, Waseem; Wang, Lu-Lu; Yan, Fang-Rong; Shang, Jing

    2014-01-01

    To investigate a possible methodology of exploiting herbal medicine and design polytherapy for the treatment of non-alcoholic fatty liver disease (NAFLD), we have made use of Cichorium glandulosum Boiss et Huet (CG), a traditional Chinese herbal medicine that has been proven to be effective in treating hepatic diseases. Here, we report that the extract of CG effectively reduced lipid accumulation under conditions of lipid overloading in vivo and in vitro (in a rat high-fat diet model and a hepG2 cell model of free fatty acid treatment). CG extract also protected hepatocytes from injury and inflammation to aid its lipid-lowering properties (in a rat high-fat diet model and a L02 cell model of acetaminophen treatment). Serum chemistry analysis accompanied by in vitro drug screening confirmed that CG-4, CG-10 and CG-14 are the lipo-effective components of CG. Western blotting analysis revealed that these components can regulate key lipid targets at the molecular level, including CD36, FATP5 and PPAR-α, thus the lipid oxidation and lipid absorption pathways. Finally, we adopted the experimental design and statistical method to calculate the best combination proportion (CG-4: CG-10: CG-14 = 2.065: 1.782: 2.153) to optimize its therapeutic effect. PMID:24797163

  13. HEALTHY PROPERTIES OF ENDIVE (CICHORIUM ENDIVIA L. DEPENDING ON THE VARIETY AND VEGETATIVE OF SEASON

    Directory of Open Access Journals (Sweden)

    Iwona Mentel

    2015-02-01

    Full Text Available The endive (Cichorium endivia L. is plant belonging to Asteraceae family. It is very popular vegetable in France, Italy and Germany. Endive because of its high content healthy components can be used in prevention against a lot of diseases. The studies were conducted on two varieties of endive: ‘Cigal’ and ‘Marconi’ during vegetative of season: 2009 and 2010. The research material was assessed for: dry matter, protein, dietary fibre, ash, selected minerals (Ca, Mg, K, P, Na, Fe, Zn, Mn, Cu levels and antioxidants (vitamin C, phenols components contents. Also, the calculation of the energy value, carbohydrates and the antioxidant potential of ABTS. were performed. The endive variety ‘Cigal’ was characterized higher content of calcium, magnesium, phosphorus, potassium, sodium, zinc and phenols components. While variety ‘Marconi’ contained more of dry matter, carbohydrates, dietary fibre, vitamin C, iron, copper and higher antioxidant activity. The studies showed statistically significant differences in terms of the content of components and antioxidant activity, between analysed varieties of vegetable and vegetation of season.

  14. Scientific Opinion on the substantiation of a health claim related to “native chicory inulin” and maintenance of normal defecation by increasing stool frequency pursuant to Article 13.5 of Regulation (EC) No 1924/2006

    DEFF Research Database (Denmark)

    Tetens, Inge

    claim related to “native chicory inulin” and maintenance of normal defecation by increasing stool frequency. The food constituent that is a subject of a claim is “native chicory inulin”. The Panel considers that “native chicory inulin”, a non-fractionated mixture of monosaccharides (< 10......%), disaccharides, inulin-type fructans and inulin extracted from chicory, with a mean DP ≥ 9, is sufficiently characterised in relation to the claimed effect. The Panel considers that maintenance of normal defecation by increasing stool frequency (provided that it does not result in diarrhoea) is a beneficial...... physiological effect. Six studies involving 86 subjects consistently showed that consumption of “native chicory inulin” at an amount of at least 12 g/day increases stool frequency. The Panel also notes the plausible mechanisms by which inulin and inulin-type fructans in “native chicory inulin” could exert the...

  15. Models Analyses for Allelopathic Effects of Chicory at Equivalent Coupling of Nitrogen Supply and pH Level on F. arundinacea, T. repens and M. sativa

    OpenAIRE

    Quanzhen Wang; Bao Xie; Chunhui Wu; Guo Chen; Zhengwei Wang; Jian Cui; Tianming Hu; Pawel Wiatrak

    2012-01-01

    Alllelopathic potential of chicory was investigated by evaluating its effect on seed germination, soluble sugar, malondialdehyde (MDA) and the chlorophyll content of three target plants species (Festuca arundinacea, Trifolium repens and Medicago sativa). The secretion of allelochemicals was regulated by keeping the donor plant (chicory) separate from the three target plant species and using different pH and nitrogen levels. Leachates from donor pots with different pH levels and nitrogen conce...

  16. Phase 1, placebo-controlled, dose escalation trial of chicory root extract in patients with osteoarthritis of the hip or knee

    OpenAIRE

    Jonnala Geetha; Branch Valerie K; Olsen Nancy J; Seskar Mira; Cooper Melisa

    2010-01-01

    Abstract Background Extracts of chicory root have anti-inflammatory properties in vitro and in animal models of arthritis. The primary objective of this investigator-initiated, Phase 1, placebo-controlled, double blind, dose-escalating trial was to determine the safety and tolerability of a proprietary bioactive extract of chicory root in patients with osteoarthritis (OA). Secondary objectives were to assess effects on the signs and symptoms of this disorder. Methods Individuals greater than ...

  17. Antimicrobial and anti-adherence activity of various combinations of coffee-chicory solutions on Streptococcus mutans: An in-vitro study

    OpenAIRE

    Rama Sharma; Vamsi Krishna L Reddy; G M Prashant; Vivek Ojha; Naveen PG Kumar

    2014-01-01

    Context: Several studies have demonstrated the activity of natural plants on the dental biofilm and caries development. But few studies on the antimicrobial activity of coffee-based solutions were found in the literature. Further there was no study available to check the antimicrobial effect of coffee solutions with different percentages of chicory in it. Aims: To evaluate the antimicrobial activity of different combinations of coffee-chicory solutions and their anti-adherence effect on Strep...

  18. The Antioxidant Properties and Inhibitory Effects on HepG2 Cells of Chicory Cultivated Using Three Different Kinds of Fertilizers in the Absence and Presence of Pesticides.

    Science.gov (United States)

    Yook, Jin-Seon; Kim, Mina; Pichiah, Pichiah BalasubramanianTirupathi; Jung, Su-Jin; Chae, Soo-Wan; Cha, Youn-Soo

    2015-01-01

    The objective of this study was to explore the antioxidant levels and anticancer properties of chicory cultivated using three different kinds of fertilizers (i.e., developed, organic, and chemical) in the presence and absence of pesticides. Phenolic phytochemicals, including total polyphenols and flavonoids, and antioxidant activities, including reducing power, ABTS+ and DPPH radical scavenging activity, were analyzed using several antioxidant assays. HepG2 cell viability was analyzed using the MTT assay. The antioxidant properties of chicory were found to increase when cultivated with chemical fertilizer in the absence of pesticides. On the other hand, antioxidant capacity was higher in chicory cultivated with eco-developed fertilizer even in the presence of pesticides. Chicory grown using eco-developed or organic fertilizer was more effective in suppressing the proliferation of HepG2 cells when compared to chicory grown with chemical fertilizer. This effect was time dependent, regardless of treatment with or without pesticides. In conclusion, the antioxidant activity of chicory were affected by the presence or absence of pesticides. However, developed and organic fertilizers showed a strong anti-proliferative effect against HepG2 cells, regardless of the presence or absence of pesticides. PMID:26140439

  19. Effects of chicory root powder on growth performance and histomorphometry of jejunum in broiler chicks

    Directory of Open Access Journals (Sweden)

    Homan Izadi

    2013-09-01

    Full Text Available In the present study, chicory root powder (CRP as growth promoter at 1% and 3% levels was supplemented in broilers’ diet to investigate the growth performance and histo- morphometry of jejunum. One hundred twenty, one-day-old male broilers were used in a completely randomized design (CRD with 3 treatments and 4 replicates (10 chicks per replicate. At the end of each period (0-10, 11-24 and 0-24 days, feed intake (FI, weight gain (WG, and feed conversion ratio (FCR were measured. At the end of experiment (day 24, one bird per replicate was sacrificed for breast weight (BW, drumstick weight (DW, and jejunum length (JL as a percentage of body weight, and histomorphometry of villus. The FI increased by 3% CRP in the 1st period (p < 0.01. The percentage of WG significantly increased at 1% during the 1st period and, in the 2nd and total periods, it increased only at 3% CRP (p < 0.05. The FCR decreased at 1% in the 1st (p < 0.04 and, at 3% in the 2nd (p < 0.01 and total periods (p < 0.05. The percentage of DW increased at 3% CRP (p < 0.05. The treatments increased the percentage of BW (p < 0.059 and, percentage of JL (p < 0.079 as well. The villus width and, crypt depth (CD at 1% and 3% CRP and, villus surface at 3% reduced. The 3% CRP increased the villus length (VL and villi number (p < 0.05 and, VL/CD (p < 0.01 and, villus surface area (p < 0.02. The percentage of leaf-like villi decreased in CRP treatments (p < 0.05. The number of goblet cells increased in CRP treatments (p < 0.01. In conclusion, chicory root powder can improve growth performance in broilers by enhancing food digestion and absorption through modification of jejunum histomorphometry.

  20. Direct anthelmintic effects of a purified chicory extract against free-living stages of Cooperia oncophora: preliminary results

    DEFF Research Database (Denmark)

    Pena-Espinoza, Miguel Angel; Williams, A.; Enemark, Heidi L.;

    2013-01-01

    methanol as the extraction medium. The resulting extract was dried and fractionated using solid-phase extraction (SPE) cartridges to enhance isolation of secondary metabolites such as sesquiterpene lactones. The obtained elutate was dried, dissolved in 100 % DMSO and serially diluted to obtain...... concentrations ranging from 5 to 0.12 mg dry matter (DM)/mL. Two Jersey calves (4 months old) mono-infected with C. oncophora were used for collection of nematode eggs. Isolated eggs were used in an egg hatch assay (EHA) with 6 concentrations of the chicory extract (tested in duplicates, final DMSO concentration...... of 2%). Thiabendazole (1000 μg/ml) and 2 % DMSO in water were used as positive and negative controls, respectively. After 48 hours of incubation (25oC), the percentage of hatched L1 in negative controls was > 90%. Hatching percentages were dramatically decreased in the presence of the chicory extract...

  1. Fructan Biosynthetic and Breakdown Enzymes in Dicots Evolved From Different Invertases. Expression of Fructan Genes Throughout Chicory Development

    Directory of Open Access Journals (Sweden)

    Wim Van den Ende

    2002-01-01

    Full Text Available Fructans are fructose-based oligo- and polymers that serve as reserve carbohydrates in many plant species. The biochemistry of fructan biosynthesis in dicots has been resolved, and the respective cDNAs have been cloned. Recent progress has now succeeded in elucidating the biochemistry and molecular biology of fructan biodegradation in chicory, an economically important species used for commercial inulin extraction. Unlike fructan biosynthetic genes that originated from vacuolar-type invertase, fructan exohydrolases (FEHs seem to have evolved from a cell-wall invertase ancestor gene that later obtained a low iso-electric point and a vacuolar targeting signal. Expression analysis reveals that fructan enzymes are controlled mainly at the transcriptional level. Using chicory as a model system, northern analysis was consistent with enzymatic activity measurements and observed carbohydrate changes throughout its development.

  2. Effects of chicory inulin on serum metabolites of uric acid, lipids, glucose, and abdominal fat deposition in quails induced by purine-rich diets.

    Science.gov (United States)

    Lin, Zhijian; Zhang, Bing; Liu, Xiaoqing; Jin, Rui; Zhu, Wenjing

    2014-11-01

    Inulin, a group of dietary fibers, is reported to improve the metabolic disorders. In the present study, we investigated the effects of chicory inulin on serum metabolites of uric acid (UA), lipids, glucose, and abdominal fat deposition in quail model induced by a purine-rich diet. In this study, 60 male French quails were randomly allocated to five groups: CON (control group), MOD (model group), BEN (benzbromarone-treated group), CHI-H (high-dosage chicory inulin-treated group), and CHI-L (low-dosage chicory inulin-treated group). The serum UA level was significantly increased in the model group from days 7 to 28, as well as triglyceride (TG) and free fatty acid (FFA) increased later in the experimental period. The abdominal fat ratio was increased on day 28. Benzbromarone can decrease UA levels on days 14 and 28. The high and low dosage of chicory inulin also decreased serum UA levels on days 7, 14, and 28. The abdominal fat ratio, activity, and protein of acetyl-CoA carboxylase (ACC) were decreased in chicory inulin-treated groups. The activities of xanthine oxidase (XOD) and fatty acid synthase (FAS) were increased in the model group and decreased in the benzbromarone and chicory inulin groups. This study evaluated a quail model of induced hyperuricemia with other metabolic disorders caused by a high-purine diet. The results indicated that a purine-rich diet might contribute to the development of hyperuricemia, hypertriglyceridemia, and abdominal obesity. Chicory inulin decreased serum UA, TG, and abdominal fat deposition in a quail model of hyperuricemia by altering the ACC protein expression and FAS and XOD activities. PMID:25314375

  3. Effects of Mushroom and Chicory Extracts on the Physiology and Shape of Prevotella intermedia, a Periodontopathogenic Bacterium

    OpenAIRE

    Caterina Signoretto; Anna Marchi; Anna Bertoncelli; Gloria Burlacchini; Francesco Tessarolo; Iole Caola; Elisabetta Pezzati; Egija Zaura; Adele Papetti; Peter Lingström; Jonathan Pratten; Spratt, David A.; Michael Wilson; Pietro Canepari

    2011-01-01

    Contrary to the common assumption that food has a negative impact on oral health, research has shown that several foods contain a number of components with antibacterial and antiplaque activity. These natural compounds may be useful for improving daily oral hygiene. In this study we evaluate the mode of antimicrobial action of fractions of mushroom and red chicory extracts on Prevotella intermedia, a periodontopathogenic bacterium. The minimal inhibitory concentration corresponded to 0.5x com...

  4. Feeding with chicory roots reduces the amount of odorous compounds in colon and rectal contents of pigs

    OpenAIRE

    Jensen, M.T.; Hansen, L L

    2006-01-01

    Sixteen pigs (eight entire males and eight females) were given individually two diets, control and control added 25% chopped chicory roots for 2 months before slaughter. Samples were taken from the contents in colon and rectum and subjected to GC-MS analysis for amount of odour impact compounds. The compounds 2-pentanone, ethylbutyrate, propylpropionate, butyric acid, ethyl-2-methylbutyrate, p-cresol, indole and skatole showed a significant difference between the two treatments. The esters...

  5. Fructan Biosynthetic and Breakdown Enzymes in Dicots Evolved From Different Invertases. Expression of Fructan Genes Throughout Chicory Development

    OpenAIRE

    Wim Van den Ende; An Michiels; Joke De Roover; Andrea Van Laere

    2002-01-01

    Fructans are fructose-based oligo- and polymers that serve as reserve carbohydrates in many plant species. The biochemistry of fructan biosynthesis in dicots has been resolved, and the respective cDNAs have been cloned. Recent progress has now succeeded in elucidating the biochemistry and molecular biology of fructan biodegradation in chicory, an economically important species used for commercial inulin extraction. Unlike fructan biosynthetic genes that originated from vacuolar-type invertase...

  6. A method for the simultaneous determination of chlorogenic acid and sesquiterpene lactone content in industrial chicory root foodstuffs.

    Science.gov (United States)

    Willeman, Honorine; Hance, Philippe; Fertin, Anne; Voedts, Najia; Duhal, Nathalie; Goossens, Jean-François; Hilbert, Jean-Louis

    2014-01-01

    A method for the simultaneous determination of free chlorogenic acids (CGA) and sesquiterpene lactones (STL) in chicory root and its dried (flour) and roasted (grain) forms is described. The method uses one extraction and one analysis for all chicory root products. Various solvents with low to high polarity, such as methanol, chloroform, or n-hexane, were tested alone, in combination in different proportions or with acidified or neutral aqueous solvent. The water/chloroform/methanol (30/30/40, v/v/v) mixture generated the best extraction yield, 21% higher than alcohol mixtures. The profiling of CGA and STL content was performed through a conventional HPLC-DAD method using a PFP core shell column in a fast single run. Good retention time and area repeatability (RDD mean % 0.46 and 5.6, resp.) and linearity (R2≥0.96) were obtained. The STL and chlorogenic acids levels determined were 254.7 and 100.2 μg/g of dry matter in the root, 792.5 and 1,547 μg/g in flour, and 160.4 and 822.5 μg/g in the roasted grains, respectively. With an average recovery of 106% and precision of 90%, this method is rapid, reproducible, and straightforward way to quantify the chlorogenic acids and STL in chicory raw material and end products. PMID:25548785

  7. Effects of Mushroom and Chicory Extracts on the Physiology and Shape of Prevotella intermedia, a Periodontopathogenic Bacterium

    Directory of Open Access Journals (Sweden)

    Caterina Signoretto

    2011-01-01

    Full Text Available Contrary to the common assumption that food has a negative impact on oral health, research has shown that several foods contain a number of components with antibacterial and antiplaque activity. These natural compounds may be useful for improving daily oral hygiene. In this study we evaluate the mode of antimicrobial action of fractions of mushroom and red chicory extracts on Prevotella intermedia, a periodontopathogenic bacterium. The minimal inhibitory concentration corresponded to 0.5x compared to the natural food concentration for both extracts. This concentration resulted in a bacteriostatic effect in mushroom extract and in a slightly bactericidal effect in chicory extract. Cell mass continued to increase even after division stopped. As regards macromolecular synthesis, DNA was almost totally inhibited upon addition of either mushroom or chicory extract, and RNA to a lesser extent, while protein synthesis continued. Cell elongation occurred after septum inhibition as documented by scanning electron microscopy and cell measurement. The morphogenetic effects are reminiscent of the mode of action of antibiotics such as quinolones or β-lactams. The discovery of an antibiotic-like mode of action suggests that these extracts can be advantageously employed for daily oral hygiene in formulations of cosmetic products such as mouthwashes and toothpastes.

  8. The influence of irradiation and packaging on the keeping quality of prepacked cut endive, chicory and onions

    International Nuclear Information System (INIS)

    The experiments with endive and chicory showed that by washing immediatedly after cutting, pink-discolouration decreased. During storage, however, this discolouration increased again. Discolouration was partially prevented by packaging in nonperforated polythene foil of 0.02 mm thereby decreasing the O2 and increasing the CO2 content in the bags. A difficulty in using non-perforated bags was that in non-irradiated products, fermentation took place caused by a high microbial count /108 g-1/. In irradiated samples no fermentation occurred probably due to the decreased microbial count. Cut onions had to be packed in perforated polythene bags because strong gas /CO2/ development took place in non-perforated bags at temperatures above 100C. The initial total viable count of endive, chicory and onions varied from 103 to 107 g-1. Irradiation with doses above 50 krad gave a reduction of this count of 3-6 log cycles through which the keeping time was lengthened by about 100% at 10-200C. The Enterobacteriaceae were mainly eliminated by irradiation doses higher than 100 krad. The optimal dose for chicory, onions and endive was found to be 50, 75, and 100 krad, respectively. Higher doses intensified discolouration and influenced unfavourably the flavour. Cooking experiments with cut onions packed in perforated bags showed that the non-irradiated product discoloured quickly into brown after cooking. By irradiation with 75-100 krad this discolouration was prevented. (F.J.)

  9. 菊苣药材质量评价的研究进展%Advances and Discussion in Studies on Quality Evaluation of Chicory

    Institute of Scientific and Technical Information of China (English)

    周俊; 张冰; 林志健; 孙博喻; 王红坡; 李丽玉

    2014-01-01

    菊苣为蒙古族和维吾尔族习用药材,其质量直接影响到它临床应用的安全性和有效性。从多个角度对菊苣药材质量评价研究进行了综述,认为菊苣药材的质量评价需从真伪/品种鉴定和品质评价两个方向着手,结合各面的研究成果,形成综合、有效的质量评价体系。%Chicory is well known in Uighur folk medicine as a cholagogic and diuretic agent to improve the ap-petite, to increase digestion and to cure liver diseases , etc.The quality of chicory will affect its safety and ef-fectiveness .This article briefly reviews the recent progress in the study of quality evaluation of chicory .We think that the quality evaluation of chicory should include its species evaluation and quality evaluation , and the combination of the researches on the quality of chicory will make it easy and effective to evaluate its quality .

  10. Phase 1, placebo-controlled, dose escalation trial of chicory root extract in patients with osteoarthritis of the hip or knee

    Directory of Open Access Journals (Sweden)

    Jonnala Geetha

    2010-07-01

    Full Text Available Abstract Background Extracts of chicory root have anti-inflammatory properties in vitro and in animal models of arthritis. The primary objective of this investigator-initiated, Phase 1, placebo-controlled, double blind, dose-escalating trial was to determine the safety and tolerability of a proprietary bioactive extract of chicory root in patients with osteoarthritis (OA. Secondary objectives were to assess effects on the signs and symptoms of this disorder. Methods Individuals greater than 50 years of age with OA of the hip or knee were eligible for trial entry. A total of 40 patients were enrolled in 3 cohorts and were treated with escalating chicory doses of 600 mg/day, 1200 mg/day and 1800 mg/day for 1 month. The ratio of active treatment to placebo was 5:3 in cohorts 1 and 2 (8 patients each and 16:8 in cohort 3 (24 patients. Safety evaluations included measurement of vital signs and routine lab tests at baseline and the end of the treatment period. Efficacy evaluations at baseline and final visits included self-assessment questionnaires and measurement of the 25-foot walking time. Results In the highest dose cohort, 18 patients who completed treatment per protocol were analyzed for efficacy. In this group, 13 patients showed at least 20% improvement in the defined response domains of pain, stiffness and global assessment: 9 of 10 (90% patients randomized to active treatment with chicory and 4 of 8 (50% patients randomized to placebo (P = 0.06. In general, the treatment was well-tolerated. Only one patient who was treated with the highest dose of chicory had to discontinue treatment due to an adverse event. Conclusions The results of this pilot study suggest that a proprietary bioactive extract of chicory root has a potential role in the management of OA and merits further investigation. Clinicaltrials.gov identifier: NCT 01010919.

  11. Effects of water extracts from chicory and BHT on the in vitro rumen degradation of feeds

    Directory of Open Access Journals (Sweden)

    Stefano Schiavon

    2010-01-01

    Full Text Available Effects of Butyl-Hydroxyl-Toluene (BHT and of Red Chicory Extract (RCE on kinetics of gas production (GP and rumen degradability values (OMd, NDFd and in vitro true OM degradability - IVTOMD of two feeds (meadow hay and corn meal were evaluated using an in vitro automatic batch system. For each feed 2 increasing dosages (0.15 and 1.5 mg/g of feed of BHT and RCE and a Control (C were tested in 4 replications and 2 incubations. First incubation lasted 72h, the 2nd one was stopped at the times on which half of GP was produced (t½, which were 9 and 16 h for corn and hay, respectively. From the supernatants of the 2nd incubation, VFA, NH3, N content of the residual NDF were analysed and the microbial N balance was computed. The 2 feeds significantly affected rumen fermentation parameters; BHT significantly increased asymptotic GP, t½ and IVTOMD (P<0.01, decreased the proportion of butyrate (P<0.01 but did not affect microbial N balance; RCE did not influence any of the parameters measured with respect to C, except for a significant increase of the estimated N available for microbes at the higher dosage.

  12. Sensory emphasis on pork quality related to the diet content of fermentable fibre-rich feedstuffs (chicory and lupine) with special emphasis on the effect on boar taint

    OpenAIRE

    Nielsen, Sandra Stolzenbach; Hansen, Laurits Lydehøj; Byrne, Derek Victor

    2007-01-01

    Boar taint in entire male pork is caused by skatole and andros-tenone and other compounds such as indole. However, female pigs also pro-duce skatole and indole. The purpose was to minimise boar taint related to skatole by feeding entire male and female pigs with fibre-rich feedstuffs. An organic, 10% dried chicory or 25% lupine diet was applied for either 7 or 14 days before slaughter. Lupines significantly reduced skatole in backfat for both genders whilst chicory showed no significant diffe...

  13. Milk production and composition of mid-lactation cows consuming perennial ryegrass-and chicory-based diets.

    Science.gov (United States)

    Muir, S K; Ward, G N; Jacobs, J L

    2014-02-01

    Dry matter intakes (DMI), nutrient selection, and milk production responses of dairy cows grazing 3 herbage-based diets offered at 2 allowances were measured. The 2 allowances were 20 (low) and 30 (high) kg of dry matter (DM)/cow per day and these were applied to 3 herbage types: perennial ryegrass (PRG) and chicory (CHIC+) monocultures and a mixed sward of chicory and perennial ryegrass (MIX). The CHIC+ diet was supplemented with alfalfa hay (approximately 2 kg of DM/cow per day) to maintain dietary neutral detergent fiber (NDF) concentration and all diets were supplemented with energy-based pellets (6 kg of DM/cow per day). Holstein-Friesian dairy cows averaging 136 ± 30 d in milk were allocated to 4 replicates of the 6 treatments using stratified randomization procedures. Cows were adapted to their experimental diets over a 14-d period, with measurements of DMI, milk yield, and composition conducted over the following 10 d. Herbage DMI was lowest (12.8 vs. 14.0 kg of DM/d) for CHIC+ compared with the MIX and PRG, although total forage intake (grazed herbage plus hay) was similar (14.0 to 15.0 kg of DM/d) across the 3 treatments. Milk production, milk protein, and milk fat concentrations were not different between herbage types. Grazed herbage DMI increased with increasing herbage allowance and this was associated with increased milk protein concentration (3.23 to 3.34%) and total casein production (41.7 to 43.6 mg/g). Concentrations of polyunsaturated fatty acids in milk fat, particularly linoleic acid, were increased in milk from cows offered the CHIC+ or the MIX diets, indicating potential benefits of chicory herbage on milk fatty acid concentrations. Although feeding CHIC+ or MIX did not increase milk yield, these herbage types could be used as an alternative to perennial ryegrass pasture in spring. PMID:24290818

  14. Chicory and Jerusalem artichoke productivity in different areas of Italy, in relation to water availability and time of harvest

    Directory of Open Access Journals (Sweden)

    Giuseppe De Mastro

    2006-06-01

    Full Text Available Inulin is an important polysaccharide synthesised by different crops, which, in the EU has been included in the system of sugar quotas since 1994. Currently, one of the major problems of the agro-industry is the need to extend the length of the sugar crop harvest season. It was therefore decided, also in relation to the increased demand for inulin, to study the two main inulin producing crops in Italy (chicory and Jerusalem artichoke, to verify yield and quality potential and stability in relation to some important agronomic factors such as irrigation and time of harvest. The work was conducted in 1999 and 2000 in four areas of Italy (Udine, Rovigo, Bologna and Bari. The effects evaluated were time of harvest (3 for chicory and 2 for Jerusalem artichoke and irrigation system (evapotranspiration replacement and dry regime, with irrigation applied only when strictly necessary on the production of storage organs, sugars and inulin in the two crops. The highest chicory root yield was in Bologna, with an average production of 65.6 t ha-1 (fresh weight, compared to Rovigo (54.4 t ha-1, Bari (46.5 t ha-1 and Udine (38.7 t ha-1. For final tuber yield in Jerusalem artichoke, Bari was the most productive environment with an average of 80 t ha-1, followed by Bologna (61 t ha-1 and Udine (55.5 t ha-1. However, when this crop is whole-plant harvested (stalks and tubers at pre-flowering, Bologna, with high stalk yields (58.7 t ha-1 appeared to be the most suitable environment. This type of harvesting was also shown to be more productive in terms of sugar and inulin yield. The total sugar content in the different organs analysed (roots, stalk and tubers was always higher in Udine compared to Bologna, for both crops. Lastly, the length of the inulin chain (average degree of polymerisation [DP] diminishes with the delaying of the harvest in both crops. The Bologna area had the highest potential in terms of chicory root production, while for the tubers yield of

  15. Stubble Height of Puna Chicory%普那菊苣留茬高度试验初报

    Institute of Scientific and Technical Information of China (English)

    李辰琼; 韩永芬; 陈培燕

    2003-01-01

    @@ 普那菊苣(Puna Chicory)属菊科多年生草本植物,莲座叶丛生型,原产于欧洲.贵州省草业科学研究所1996年经省农业厅外经处从新西兰国家草地农业研究所引进种植,并获成功.2002年对普那菊苣不同留茬高度与产量和再生速度的关系进行了研究.

  16. Studies on HPLC Fingerprint of Chicory Root%菊苣根HPLC指纹图谱的研究

    Institute of Scientific and Technical Information of China (English)

    吴丽丽; 张冰; 刘小青; 朱文静; 林志健

    2012-01-01

    Objective To establish the HPLC fingerprint of chicory root for its quality evaluation. Methods HPLC fingerprint was achieved on an Agilent ZORBAX SB-C18 column with methanol- 0.1 % phosphoric acid solution as mobile phase in gradient elution and 254 nm as detection wavelength. The time for analysis was 80 minutes. Similarity analysis of HPLC fingerprint was performed on the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine. Results Thirteen common peaks were detected in 7 batches of chicory root. The similarities of stability, instrument precision and reproducibility were over 0.90. Conclusion The fingerprints of chicory root on market have high similarity, and HPLC fingerprint can be used for quality evaluation of chicory root.%目的 建立菊苣根的HPLC指纹图谱,探讨指纹图谱在菊苣质量评价方面的应用.方法 采用Agilent ZORBAX SB-C18为色谱柱,以甲醇-0.1%磷酸水溶液为流动相,在80 min内梯度洗脱,检测波长为254 nm.采用中药色谱指纹图谱相似度评价系统生成对照图谱,并进行相似度分析.结果 7批菊苣根药材的指纹图谱包含13个共有峰,对7个不同产地的菊苣根进行方法学考察,其稳定性、仪器精密度、试验方法重复性的相似度均在0.9以上.结论 菊苣根药材的指纹图谱有较高的相似度,建立了菊苣根的HPLC指纹图谱分析方法.

  17. Effects of feeding finisher pigs with chicory or lupine feed for one week or two weeks before slaughter with respect to levels of Bifidobacteria and Campylobacter

    DEFF Research Database (Denmark)

    Jensen, Annette Nygaard; Hansen, L. L.; Baggesen, Dorte Lau;

    2013-01-01

    This study aimed to assess whether inclusion of chicory or lupine (prebiotics) in the diet of pre-slaughter pigs for just 1 or 2 weeks could change the composition of their intestinal microbiota, stimulate the growth of bifidobacteria and help to lower the amount of thermoplilic Campylobacter spp....... (mainly Campylobacter jejuni and Campylobacter coli), which are a major cause of food-borne infections in humans. A total of 48 pigs that had an initial live weight of 90 kg were fed with either a lupine (organic concentrate with 25% blue lupine seeds), chicory (organic concentrate with 10% dried chicory......- and chicory-fed pigs (mean log10 2.9 v. 4.1 CFU/g; P <0.05). The qPCR analysis showed that feeding with lupines resulted in higher levels of bifidobacteria in caecum as compared with the other diets (P <0.05). T-RFLP analysis showed that four of the most abundant bacteria with terminal restriction...

  18. Antimicrobial and anti-adherence activity of various combinations of coffee-chicory solutions on Streptococcus mutans: An in-vitro study

    Directory of Open Access Journals (Sweden)

    Rama Sharma

    2014-01-01

    Full Text Available Context: Several studies have demonstrated the activity of natural plants on the dental biofilm and caries development. But few studies on the antimicrobial activity of coffee-based solutions were found in the literature. Further there was no study available to check the antimicrobial effect of coffee solutions with different percentages of chicory in it. Aims: To evaluate the antimicrobial activity of different combinations of coffee-chicory solutions and their anti-adherence effect on Streptococcus mutans to glass surface. Materials and Methods: Test solutions were prepared. For antimicrobial activity testing, tubes containing test solution and culture medium were inoculated with a suspension of S. mutans followed by plating on Brain Heart Infusion (BHI agar. S. mutans adherence to glass in presence of the different test solutions was also tested. The number of adhered bacteria (CFU/mL was determined by plating method. Statistical Analysis: Statistical significance was measured using one way ANOVA followed by Tukey′s post hoc test. P value < 0.05 was considered statistically significant. Results: Pure chicory had shown significantly less bacterial count compared to all other groups. Groups IV and V had shown significant reduction in bacterial counts over the period of 4 hrs. Regarding anti-adherence effect, group I-IV had shown significantly less adherence of bacteria to glass surface. Conclusions: Chicory exerted antibacterial effect against S. mutans while coffee reduced significantly the adherence of S. mutans to the glass surface.

  19. NaCl胁迫对菊苣种子萌发的影响%Effect of NaCI Stress on Seed Germination of Chicory

    Institute of Scientific and Technical Information of China (English)

    郭丹丹

    2012-01-01

    Solutions of NaC1 concentration of 0 mmol/L ,50 mmol/L, 100 mmol/L, 150 mmol/L ,200 mmol/L,250 mmol/L,300 mmol/L were used to handle the three chicory seeds, the physiological indicators such as germination rate ,germinability, root length, seedling length and fresh weight of chicory were studied.Results indicated that seed germination rate and concentration of NaC1 showed a significant negative correlation. 150 mmol/L NaC1 was semi-lethal salt concentration for chicory seeds. 250 mmol/L NaC1 was the limit salt concentration for chicory seeds. The effect of 100 mmol/L NaC1 on the growth after seed germination of chicory was significant.%分别用0、50、100、150、200、250、300mmol/L的NaCl溶液对3种菊苣种子进行处理,并对发芽率、发芽势、根长、苗长和鲜重等生理指标进行研究。结果表明:种子萌发率与NaC1浓度呈明显的负相关。150mmol/L的NaCl浓度为菊苣种子耐盐半致死浓度,250mmol/L的NaCl浓度为菊苣种子耐盐极限浓度。100mmol/L的NaCl对菊苣种子萌发后的生长影响显著。

  20. Post-harvested forcing technique of chicory root%菊苣根采后软化技术研究

    Institute of Scientific and Technical Information of China (English)

    赵丛枝; 唐霞

    2012-01-01

    The impact of chicory root test for softening was studied, The results show that the chicory roots which were packed with PE film of 0.04 mm thick could be stored 150 days better at 0 ℃ and -2 ℃, and the storage effect was almost same. The quality of chicon produced by chicory roots that were stored at 0 ~C and -2 ~C, was not distinct through forcing. In the mode of aerating during forcing, the weight, yield and size of chicon were increased visibly and the lateral roots were developed. The weight, yield, size, height and diameter of chicon were increased by forcing in nutrient solution of complex fertilizers. The density of chicon was reduced because the size of chicon increased. The effect of light on growth of chicon was not distinct in short time, but the weight, yield and density of chicon decreased, the bitterness and chlorophyll content increased because of light of long time.%对影响菊苣根软化的一些因素进行了研究。结果表明,将菊苣根用0.04mm厚的PE袋包装,在0、-2℃条件下可以很好地贮藏150d,且2个温度下的贮藏效果差异较小。在菊苣根的软化过程中,向软化用水中充入空气,可以明显地提高芽球的质量、产率、体积,并且菊苣根的侧根生长的也比较发达;采用营养液进行菊苣根的软化,同样可以显著提高菊苣芽球的质量、产率、体积、高度和直径,但是由于芽球的膨大,芽球的结球度降低;短时间的光照对菊苣的软化影响不大,较长时间的光照不仅对芽球的生长产生影响,使芽球质量、产率降低、结球度下降,而且可以明显增加芽球的苦味和叶绿素的含量。

  1. Feeding preferences and voluntary feed intake of dairy cows: Effect of conservation and harvest time of birdsfoot trefoil and chicory.

    Science.gov (United States)

    Lombardi, D; Vasseur, E; Berthiaume, R; DeVries, T J; Bergeron, R

    2015-10-01

    Bioactive forages contain compounds, such as tannins, that are active against pathogens. They have been successfully used in ruminants to control parasite infections. Because cattle may find bioactive forages unpalatable, it is of interest to know if an afternoon harvest time, which has been shown to increase the percentage of nonstructural carbohydrates (NSC), hence palatability, may mitigate this. The objectives of this study were to quantify voluntary intake and preference of dairy cows for 2 bioactive forages, harvested in the morning and evening, in addition to determining their time spent grazing on each forage species. The forage species evaluated were fresh chicory harvested at 0700 h (FCAM) and 1800 h (FCPM), fresh birdsfoot trefoil harvested at 0700 h (FBAM) and 1800 h (FBPM), birdsfoot trefoil baleage harvested the previous summer at 0700 h (BBAM) and at 1800 h (BBPM), and third-cut alfalfa baleage harvested the previous summer and used as control (CON). Single forages were offered ad libitum in 30-min tests to 14 dairy cows to determine intake in a 7 × 7 Latin square (experiment 1). Every possible pair of forages (21 pairs) was then presented for a 30-min test to 8 different dairy cows, and feed intake was measured (experiment 2). Finally, time spent grazing on chicory and birdsfoot trefoil was measured on 12 dairy cows (experiment 3). The tests consisted of 2 d of restriction on 1 of the 2 fields for 1h, and 2 d of free-choice sessions (1h) between the 2 fields adjacent to each other. Grazing time and location of the animals on the field was assessed through 2-min scan sampling. In experiment 1, the highest voluntary intakes were for CON, BBPM, and BBAM. In experiment 2, BBPM was preferentially consumed over all other forages followed by CON and BBAM. Multidimensional scaling showed that preference for BBPM, CON, and BBAM in dimension 1 was positively associated with dry matter and nitrogen content, and negatively associated with hemicellulose and

  2. Produtividade da chicória (Cichorium endivia L. em função de tipos de bandejas e idade de transplante de mudas Endive (Cichorium endivia L. yield in function of tray types and seedlings age at transplanting

    Directory of Open Access Journals (Sweden)

    Marie Yamamoto Reghin

    2007-06-01

    Full Text Available A chicória (Cichorium endivia L. tem apresentado aumento crescente de cultivo no Brasil, constituindo-se atualmente numa hortaliça folhosa importante, consumida principalmente como salada. No entanto, ainda são escassas as informações existentes sobre a cultura. O objetivo desse experimento foi avaliar os efeitos de tipos de bandejas e idade de transplante de mudas na produtividade de chicória. As mudas foram produzidas sob ambiente protegido, com a cultivar AF-254 (SAKATA. O delineamento experimental foi o de blocos casualizados, com quatro repetições, sendo os tratamentos arranjados em esquema fatorial 4 x 3, sendo quatro idades de transplante de mudas (25, 30, 35 e 42 dias após a semeadura e três tipos de bandejas, com 128, 200 e 288 células. O substrato usado foi o Plantmax®. As semeaduras foram realizadas entre os dias 11/04/05 a 28/04/05, com intervalo de sete dias da primeira semeadura e de cinco dias na segunda, terceira e quarta semeadura. O transplante foi efetuado no dia 23/05/04, em parcelas compostas de três fileiras de plantas arranjadas no espaçamento 0,30 x 0,30 m. A colheita foi realizada de acordo com a maturidade da planta. Mudas obtidas da bandeja de 128 células foram superiores independentemente da idade. As maiores diferenças encontradas foram entre as bandejas de 128 com as de 288 células. O ponto de máximo desenvolvimento das mudas nas características avaliadas foi entre 30 e 40 dias, nos três tipos de bandejas. A maior produtividade da chicória foi proveniente de mudas obtidas da bandeja de 128 células e as idades de 30 e de 35 dias. A alta produtividade obtida com a muda da bandeja de 128 células compensa o maior investimento na produção de mudas.Endive (Cichorium endivia L. production has increased in Brazil, where it is currently considered an important vegetable, being consumed mainly as salad. However, the studies about this crop are scarce. This work aimed to avaluate the effects of tray

  3. Influence of sodium fluoride and malonate on the respiration and metabolism of di- and tricarboxylic acids in chicory leaves

    Energy Technology Data Exchange (ETDEWEB)

    Mazurova, T.A.; Tenchurina, N.S.

    1975-01-01

    Fluoride has a weak inhibitory effect on leaf respiration and the production of pyruvic acid. During 24 h the accumulation of citric and malic acids without effect on the tartaric acid was demonstrated. The inhibitory effect of fluoride was increased by pyruvate. The pyruvic acid stimulates both CO/sub 2/-output and O/sub 2/-consumption. The accumulation of citric and malic acids is influenced by pyruvic acid, but the tartaric acid content decreases. The malonate has a strong inhibitory effect on leaf respiration and induces the accumulation of succinic acid, the strong decrease of citric and malic acids but the decrease of the content of tartaric acid. The inhibitory effect of malonate was eliminated by succinate. Glucolysis is probably not the main pathway in the production of pyruvate in the chicory leaves.

  4. Effect of soluble and insoluble fibers within the in vitro fermentation of chicory root pulp by human gut bacteria.

    Science.gov (United States)

    Ramasamy, Uttara S; Venema, Koen; Schols, Henk A; Gruppen, Harry

    2014-07-16

    The aim of this research was to study the in vitro fermentation of chicory root pulp (CRP) and ensiled CRP (ECRP) using human fecal inoculum. Analysis of carbohydrate levels in fermentation digests showed that 51% of all CRP carbohydrates were utilized after 24 h of fermentation. For ECRP, having the same cell wall polysaccharide composition as CRP, but with solubilization of 4 times more of CRP pectin due to ensiling, the fermentation was quicker than with CRP as 11% more carbohydrates were utilized within the first 12 h. The level of fiber utilization for ECRP after 24 h was increased by 8% compared to CRP. This effect on fiber utilization from ECRP seemed to arise from (i) increased levels of soluble pectin fibers (arabinan, homogalacturonan, and galactan) and (ii) ahypothesized more open structure of the remaining cell walls in ECRP, which was more accessible to degradation than the CRP cell wall network. PMID:24967835

  5. Phyto-oestrogens in herbage and milk from cows grazing whiteclover, red clover, lucerne or chicory-rich pastures

    DEFF Research Database (Denmark)

    Andersen, C; Nielsen, T S; Purup, S; Kristensen, T; Eriksen, J; Søegaard, K; Sørensen, J; Fretté, X C

    2009-01-01

    A grazing experiment was carried out to study the concentration of phyto-oestrogens in herbage for cattle and in milk during two periods (May and June). Forty-eight Danish Holstein cows were divided into four groups with four treatment diets; white clover, red clover, lucerne and chicory...... clover and 238 to 466 mg/kg DM for the other three herbages mainly due to a much higher concentration of biochanin A, formononetin and glycitein in red clover. In the milk, the total concentration of phyto-oestrogens was 253 to 397 μg/l for red clover milk and 56 to 91 μg/l in the milk from the other...... three treatments. This was especially due to a higher concentration of equol, daidzein and formononetin in the red clover milk. The concentration of biochanin A was significantly higher in milk from the red clover treatment in May while no differences were observed in June. Enterodiol was similar across...

  6. Physiological effects of chicory root preparations with various levels of fructan and polyphenolic fractions in diets for rats.

    Science.gov (United States)

    Juśkiewicz, Jerzy; Zary-Sikorska, Ewa; Zduńczyk, Zenon; Król, Bogusław; Jurgoński, Adam

    2011-02-01

    The experiment was aimed at studying the effects of easily fermentable oligosaccharides and phenolic compounds from chicory root meal (CRM) on the fermentative processes in the caecum, the antioxidative status and the lipoprotein profile of rats. Five different diets were fed ad libitum to 40 Wistar rats (eight animals per group, individually housed): a control group (C); group PCM (10% processed CRM, deprived of polyphenolic fraction); group PCMO (8% processed CRM and 1.6% oligofructose); group UCM (10% unprocessed CRM); and group FP (8.3% fructan-polyphenol concentrate from CRM). Diets PCM, PCMO, UCM and FP induced favourable metabolic changes in the caecum, blood lipid profile and the antioxidative status of the body. In the caecum, the experimental diets increased the production of volatile fatty acids (VFA) and acidification of digesta as well as a decrease in the ammonia concentration and bacterial beta-glucuronidase activity. In blood serum, the total cholesterol concentration was reduced and, simultaneously, the proportion of HDL in the total cholesterol concentration was increased. The presence of the polyphenolic fraction in the unprocessed meal (diets UCM and FP) evoked a significant increase in the total antioxidative status in blood serum. Dietary fibre and the polyphenolic fraction present in diet UCM and the FOS-polyphenol concentrate in diet FP did not exhibit an antagonistic activity regarding the physiological parameters analysed, except for in the intensity of caecal fermentation. The results of the experiment point to the benefits of dietary supplementation with chicory preparations containing both prebiotic saccharides and polyphenolic compounds, which enable us to take advantage of the physiological traits of both components. PMID:21452615

  7. The Antioxidant Properties and Inhibitory Effects on HepG2 Cells of Chicory Cultivated Using Three Different Kinds of Fertilizers in the Absence and Presence of Pesticides

    OpenAIRE

    Jin-Seon Yook; Mina Kim; Pichiah BalasubramanianTirupathi Pichiah; Su-Jin Jung; Soo-Wan Chae; Youn-Soo Cha

    2015-01-01

    The objective of this study was to explore the antioxidant levels and anticancer properties of chicory cultivated using three different kinds of fertilizers (i.e., developed, organic, and chemical) in the presence and absence of pesticides. Phenolic phytochemicals, including total polyphenols and flavonoids, and antioxidant activities, including reducing power, ABTS+ and DPPH radical scavenging activity, were analyzed using several antioxidant assays. HepG2 cell viability was analyzed using t...

  8. The effect of a diet with fructan-rich chicory roots on intestinal helminths and microbiota with special focus on Bifidobacteria and Campylobacter in piglets around weaning

    OpenAIRE

    Jensen, Annette Nygaard; Mejer, Helena; Mølbak, Lars; Langkjær, Maria; Jensen, Tim Kaare; Angen, Øystein; Martinussen, Torben; Klitgaard, Kirstine; Baggesen, Dorte Lau; Thamsborg, Stig Milan; Roepstorff, Allan

    2010-01-01

    The restrictions on the use of antibiotic and anthelmintic treatments in organic pig farming necessitate alternative non-medical control strategies. Therefore, the antibiotic and parasite-reducing effect of a fructan-rich (prebiotic) diet of dried chicory was investigated in free-ranging piglets. Approximately half of 67 piglets from 9 litters were experimentally infected with Ascaris suum and Trichuris suis in the suckling period (1 to 7 weeks of age) and 58 of the piglets were challenged da...

  9. Dietary supplementation with dried chicory root triggers changes in the blood serum proteins engaged in the clotting process and the innate immune response in growing pigs.

    Science.gov (United States)

    Lepczynski, A; Herosimczyk, A; Ozgo, M; Skomial, J; Taciak, M; Barszcz, M; Berezecka, N

    2015-02-01

    The aim of the study was to characterize the systemic immune and metabolic alterations in the blood serum of growing pigs in response to a dietary supplementation with 4% of dried chicory roots. This was achieved by examining the influence of the experimental diet on serum protein changes especially these related with immunology and lipid metabolism. Serum proteins with the isoelectric point ranging from pH 3.0 to 10.0 were separated using high resolution two-dimensional electrophoresis. As a result, we found that experimental diet triggered significant changes in 37 protein spots. Of these, 14 were up-regulated, whereas 23 showed down-regulation. Of 37 significantly altered protein spots, 24 were successfully identified, representing 14 distinct gene products. Implementation of the dried chicory roots into the diet of growing pigs caused a significant down-regulation of apolipoprotein C-II complement component C6, C-reactive protein, CD14 antigen, C4b binding protein α and β chains, and fibrinogen. Piglets fed experimental diet had similar IgA, IgG and IgM concentrations, although the level of IgM tended to be lower compared to the control group. It is concluded that diet supplemented with 4% of dried chicory root may exert anti-inflammatory properties and affect lipid metabolism in growing pigs. PMID:25716964

  10. Dicty_cDB: VFM645 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available VF (Link to library) VFM645 (Link to dictyBase) - G20229 DDB0215888 Contig-U09876-1 - (Link to O ... S) chicory Cichorium in... 60 5e-12 3 ( EL342921 ) CCE L10914.b1_D17.ab1 CCE (LMS) endive Cichorium en... 6 ... 0 5e-12 3 ( EL371213 ) CCE L13227.b1_F19.ab1 CCE (LMS) endive Cichorium en... 6 ...

  11. 菊苣根采后生理及贮藏技术研究%physiological Characteristics and Storage Technology of Chicory Root

    Institute of Scientific and Technical Information of China (English)

    周志平

    2012-01-01

    [目的]研究菊苣根在贮藏期间的生理变化,得出菊苣根最佳的贮藏条件,为芽球菊苣的周年供应提供条件.[方法]采用分阶段随机法取样,采用直接滴定法进行总糖和还原糖的测定;参照Bradford的测定方法,以牛血清白蛋白作标准曲线测定可溶性蛋白;统计法计数腐烂率.[结果]在0和-2℃的贮藏条件下,菊苣根中的非还原糖和还原糖含量不尽相同,但总糖的含量(千重)几乎是一样的;两个温度下,菊苣根中可溶性蛋白含量在整个贮藏阶段差异较小;随着贮藏时间的延长,0℃下的菊苣根的腐烂指数越来越大于-2℃下的菊苣根.[结论]-2℃的贮藏条件适于菊苣根更长期的贮藏.%[ Objective ] To study the psychological characteristics of chicory root, so as to obtain its best storage conditions and supply chicory for the anniversary. [Method] The multistage random sampling method and direct titration method were adopted for the determination of total sugar and reducing sugar. The soluble protein content was determined according to the bradford determination method; the rate of decay was counted by the statistical method. [ Result] Under 0 and -2 ℃ storage conditions, the non-reducing sugar and reducing sugar in the chicory root were different, but the content of tutul sugar (dry weight) was basically the same; under the two temperatures, the soluble prolein content changed liltle during the whole storage conditions; with the prolonging storage time, the decay index of chicory root under 0 ℃ was gradually larger than that under -2 ℃. [Conclusion] -2 ℃ is better for the long-terra storage of chicory root.

  12. Dried chicory root modifies the activity and expression of porcine hepatic CYP3A but not 2C – Effect of in vitro and in vivo exposure

    DEFF Research Database (Denmark)

    Rasmussen, Martin Krøyer; Zamaratskaia, Galia; Andersen, Bente;

    2012-01-01

    Hepatic cytochrome P450 expression and activity are dependent on many factors, including dietary ingredients. In the present study, we investigated the in vivo and in vitro effect of chicory root on hepatic CYP3A and 2C in male pigs. Chicory feeding increased the expression of CYP3A29 mRNA but not...... CYP2C33. Correspondingly, CYP3A activity was increased by chicory feeding, while CYP2C activity was not affected. Additionally, the in vitro effect of chicory extract on the CYP3A activity was investigated. It was shown that CYP3A activity in the microsomes from male pigs was inhibited, but this...... effect was eliminated by pre-incubation. In both male and female pigs the CYP3A activity was increased in the presence of chicory after pre-incubation. Furthermore, gender-related differences in mRNA expression and activity were observed. CYP3A mRNA expression was greater in female pigs; this was not...

  13. EVOLVED GAS ANALYSIS (COUPLED TG-DSC-FT-IR APPLIED IN THE STUDY OF FRUCTOOLIGOSACCHARIDES FROM CHICORY

    Directory of Open Access Journals (Sweden)

    Roberta de Souza Leone

    2014-08-01

    Full Text Available EGA (Evolved Gas Analysis is a group of coupled techniques (in this case TG-DSC and FT-IR that was used to provide information about the thermal and calorimetric behavior of standard fructooligosaccharides (FOS from chicory. These FOS are found in several foods (tuber, roots, fruits, leaves, cereals, etc. and have been the subject of several studies. In the present study thermogravimetry (TG allowed the characterization of FOS a standard (Sigma-Aldrich, in which the weight loss can be observed in three stages (m 7.56, 55.53 and 36.53%, respectively. The simultaneous use of DSC showed endo and exothermic events in temperature characteristics and in agreement with TG curves. The enthalpies of the main stages of decomposition were calculated: ΔHdehydr 260 J g-1 and ΔHdec 410 J g-1. From the FT-IR spectrum of the volatiles was possible to characterize the main bands, which confirmed CO and CO2 as a result of thermal decomposition.

  14. Comparison of properties of new sources of partially purified inulin to those of commercially pure chicory inulin.

    Science.gov (United States)

    Mudannayake, Deshani C; Wimalasiri, Kuruppu M S; Silva, Kahandage F S T; Ajlouni, Said

    2015-05-01

    Newly developed inulin powders were prepared from roots of Asparagus falcatus (AF) and Taraxacum javanicum (TJ) plants grown in Sri Lanka. Inulin content, analyzed by enzymatic spectrophotometric as well as high-performance liquid chromatographic methods, showed that AF and TJ inulin powders contain 65.5% and 45.4% (dry wt) inulin, respectively, compared with 72% dry wt in the commercially available chicory inulin. Treating the AF and TJ inulin powders using ion exchange techniques significantly (P < 0.05) reduced their contents of micro (Zn, Cu, Mn, Fe) and macro (Na, K, Ca, Mg) elements. Enzymatic hydrolysis of inulin into fructose and glucose by fructanase, and FT-IR analyses proved that the developed AF and TJ inulins have characteristic molecular composition similar to commercial inulin. TJ inulin contained significantly (P < 0.05) greater amounts of total phenolics (4.37 mg GAE/g), total flavonoids (2.79 mg QE/g), and antioxidant capacity (833.11 mM TE/g) than AF inulin, which contained 1.33 mg GAE/g of total phenolics, 0.43 mg QE/g of total flavonoids, and 406.26 mM TE/g antioxidant capacity. The current study suggests that the newly developed inulin from AF and TJ roots could be used as an alternative commercial source of inulin for the food industry. PMID:25847760

  15. Scientific Opinion on the substantiation of a health claim related to “native chicory inulin” and maintenance of normal defecation by increasing stool frequency pursuant to Article 13.5 of Regulation (EC) No 1924/2006

    OpenAIRE

    EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA)

    2015-01-01

    Following an application from BENEO-Orafti S.A., submitted pursuant to Article 13.5 of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim related to “native chicory inulin” and maintenance of normal defecation by increasing stool frequency. The food constituent that is a subject of a claim is “native chicory inulin”. The Panel consi...

  16. ALLELOPATHIC POTENTIAL OF Portulaca oleracea L. SEED EXTRACTS ON GERMINATION AND SEEDLING GROWTH OF Cichorium endivia L., Lactua sativa L., Echinochloa crus-galli L., AND Brassica tournefortii Gouan

    OpenAIRE

    Hanaa Fahmy Shehata

    2014-01-01

    Present study was formulated to find out the phytotoxic effects of different concentrations of water, ethyl acetate, petroleum ether and methanol extracts of Portulaca oleracea seeds on germination, germination index and seedlings growth of Cichorium endivia, Lactuca sativa Echinochoa crus-galli, and Brassica tournefortii. Also the total phenolics and flavonoids were determined. Results indicated that the responses of allelopathic effects were depends on extract type and conce...

  17. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2013. Scientific Opinion on the substantiation of a health claim related to a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf chard, red Swiss chard, golden Swiss chard and white Swiss chard and protection of blood lipids from oxidative damage pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    OpenAIRE

    Tetens, Inge

    2013-01-01

    Following an application from Italsur s.r.l., submitted pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Italy, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim related to a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf chard, red Swiss chard, golden Swiss chard and white Swiss chard and protection of blood ...

  18. EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2013. Scientific Opinion on the substantiation of a health claim related to a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf chard, red Swiss chard, golden Swiss chard and white Swiss chard and maintenance of normal blood LDL-cholesterol concentration pursuant to Article 13(5) of Regulation (EC) No 1924/2006

    OpenAIRE

    Tetens, Inge

    2013-01-01

    Following an application from Italsur s.r.l., submitted pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Italy, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim related to a combination of red spinach, green spinach, red chicory, green chicory, green leaf chard, red leaf chard, red Swiss chard, golden Swiss chard and white Swiss chard and maintenance of norma...

  19. Изучение гепатопротекторной активности экстракта цикория (Cichorium intybus L.)

    OpenAIRE

    Yezerska, O. I.; Havryliuk, I. M.; Havryliuk, O. M.; Niektiehaiev, I. O.; Kalyniuk, T. H.

    2014-01-01

    Приведены результаты определения гепатопротекторной активности экстракта цикория на модели хронического повреждения печени тетрахлорметаном. Показано, что экстракт цикория оказывает гепатопротекторное действие, снижая активность аланинаминотрансферазы, аспартатаминотрансферазы, а также уменьшая признаки повреждения в печени....

  20. Viabilidad del desarrollo de alimentos funcionales frescos por incorporación de aloe vera a la matriz estructural de endibia (Cichorium intybus L. var. foliosum), brócoli (Brassica oleracea var. Itálica), coliflor (Brassica oleracea var. Botrytis) y zanahoria (Daucus carota L.) mediante la técnica de impregnación a vacío

    OpenAIRE

    SANZANA RAMOS, SIGRID XIMENA

    2010-01-01

    El vínculo que une la alimentación y la salud es un aspecto que desde hace algunos años marca la evolución de la industria de los alimentos. El origen de los alimentos funcionales, es fruto de esta preocupación, ampliando el concepto de alimentación a la posibilidad de mantener y/o mejorar la salud. Los alimentos funcionales que incluyen, según definición del ILSI, a cualquier alimento o ingrediente del mismo que pueda producir para la salud un beneficio adicional al del valor nutritivo de...

  1. Strukturchemische und sensorische Charakterisierung von Guaiansesquiterpenlactonen in Chicorée (Cichorium intybus L. var. foliosum Hegi) und C17-21-Oxylipinen in Avocado (Persea americana Mill. cv. Hass)

    OpenAIRE

    Degenhardt, Andreas Georg

    2012-01-01

    Die vorliegende Dissertation beschreibt die molekularen Ursachen für die Bitterkeit von Chicorée und erhitzter Avocado. Nach der Identifizierung von Guaiansesquiterpenlactonen in Chicorée-Salatblättern und C17-21-Oxylipinen in erhitzter Avocadopulpe mittels instrumentell-analytischer Techniken erfolgten Untersuchungen zur sensorischen Wirksamkeit dieser Bitterstoffe. Mittels molekular-sensorischer Techniken konnten den C17-21-Oxylipinen mit einer 1-Acetoxy-Partialstruktur ein Kokumi-Effekt zu...

  2. First report of phytophagous stink bug in chicory crop Primeiro registro de percevejo fitófago em cultura de almeirão

    Directory of Open Access Journals (Sweden)

    Diones Krinski

    2013-01-01

    Full Text Available This study reports the first occurrence of the stink bug Edessa meditabunda (F. (Heteroptera: Pentatomidae on chicory in Pará State, Brazil (7°7'45.59"S 55°23'20.99"W. Samples were taken at Florentino Farm, municipality of Novo Progresso from a plot of plants (5mx1,25m. Sixty-eight chicory plants were sampled and 1.649 adults, 636 nymphs and 1.012 egg masses were obtained.Esta nota relata a primeira ocorrência do percevejo Edessa meditabunda (F. (Heteroptera: Pentatomidae na cultura de almeirão, no Estado do Pará (7°7'45.59"S 55°23'20.99"W. Na Fazenda Florentino, município de Novo Progresso, em um canteiro (5mx1,25m, foram coletados percevejos adultos, ninfas e posturas. Em 68 plantas de almeirão, coletaram-se 1.649 adultos, 636 ninfas e 1.012 posturas.

  3. Herbage dry-matter production and forage quality of three legumes and four non-leguminous forbs grown in single-species stands

    DEFF Research Database (Denmark)

    Elgersma, A.; Søegaard, Karen; Jensen, Søren Krogh

    2014-01-01

    Agronomic data on most broad-leaved species of grasslands are scarce. The aim of this study was to obtain novel information on herbage DM yield and forage quality for several forb species, and on species differences and seasonal patterns across harvests and in successive years. Four non-leguminou......Agronomic data on most broad-leaved species of grasslands are scarce. The aim of this study was to obtain novel information on herbage DM yield and forage quality for several forb species, and on species differences and seasonal patterns across harvests and in successive years. Four non......-leguminous forbs [salad burnet (Sanguisorba minor), caraway (Carum carvi), chicory (Cichorium intybus) and ribwort plantain (Plantago lanceolata)] and three leguminous forbs [yellow sweet clover (Melilotus officinalis), lucerne (Medicago sativa) and birdsfoot trefoil (Lotus corniculatus)] and a perennial ryegrass...... common feature was found within the functional groups of non-leguminous forbs and leguminous forbs, other than higher crude protein contents (198–206 g kg−1 DM) in the legumes. DM yield and fibre content were lowest in October. Digestibility declined with higher temperature and increasing fibre content...

  4. In situ biomonitoring of the genotoxic effects of mixed industrial emissions using the Tradescantia micronucleus and pollen abortion tests with wild life plants: Demonstration of the efficacy of emission controls in an eastern European city

    International Nuclear Information System (INIS)

    Aim of the study was to monitor changes of genotoxic activity of urban air caused by an incinerator and a petrochemical plant in Tradescantia micronucleus (Trad-MCN) and pollen fertility assays with wild plants (Chelidonium majus, Clematis vitalba, Cichorium intybus, Linaria vulgaris, Robinia pseudoacacia). While in the first sampling period (1997-2000) significantly (on average 80%) more MN were found at the polluted site in comparison to controls from a rural area, no significant effects were observed during a later period (between 2003 and 2005). A similar pattern was observed in the pollen abortion assays in which the most pronounced effects were found in chicory and false acacia. The differences of the results obtained in the two periods can be explained by a substantial reduction of air pollution by use of new technologies. In particular the decrease of SO2 emissions may account for the effects seen in the present study. - Air pollution caused by industrial emissions induced micronuclei in Tradescantia and increased pollen abortion in wild plant species

  5. [Inulin and derivates as key ingredients in functional foods].

    Science.gov (United States)

    Madrigal, Lorena; Sangronis, Elba

    2007-12-01

    Inulin is a non-digestible carbohydrate that is contained in many vegetables, fruits and cereals. It is industrially produced from the chicory's root (Cichorium intybus) and it is widely used as ingredient in functional foods. Inulin and its derivate compounds (oligofructose, fructooligosaccharides) are usually called fructans, as they are basically based on linear fructose chains. This review presents a description of inulin and its most common derivate compounds: chemical structure, natural sources, physic-chemical properties, technological functionality, industrial manufacturing, analytical method for determination and health benefits: prebiotic, dietary fiber, low caloric value, hypoglycemic action, enhancement of calcium and magnesium bioavailability. Potential benefits: lipid parameters regulation, reduction of colon cancer risk and others, improvement of immune response, intestinal disorders protection. From technological point of view, these compounds exhibit a variety of properties: thickener, emulsifier, gel forming, sugar and fat substitute, humectant, freezing point depression. Inulin and derivates are been used in pharmaceutical, chemical and processing industry as technological additives and excipients. They are also been used for animal feeding. They are been considered as "bioactive" compounds to be proposed as future packaging material. Fructans are proposed to be classified as "functional fiber", according to recent concepts based on physiological effects on individuals. This review of inulin and its derivates was useful to show the broad boundaries of these compounds in the food industry and why they may be considered as key ingredients in the expanding functional food market. PMID:18524324

  6. Interrelations between herbage yield, α-tocopherol, β-carotene, lutein, protein, and fiber in non-leguminous forbs, forage legumes, and a grass-clover mixture as affected by harvest date.

    Science.gov (United States)

    Elgersma, Anjo; Søegaard, Karen; Jensen, Søren Krogh

    2015-01-21

    Pastures with diverse botanical composition may enhance animal-derived product quality. A recent study demonstrated high vitamin concentrations and yields in some forb species. The objectives of the present study were to investigate interrelations between herbage yields, vitamin concentrations, protein and fiber contents and analyze the effect of harvest date. We hypothesized that interrelations would be similar across investigated forage species. Four nonleguminous forbs: salad burnet (Sanguisorba minor), caraway (Carum carvi), chicory (Cichorium intybus), and ribwort plantain (Plantago lanceolata), three legumes: yellow sweet clover (Melilotus officinalis), lucerne (Medicago sativa), and birdsfoot trefoil (Lotus corniculatus) and a perennial ryegrass (Lolium perenne)-white clover (Trifolium repens) mixture were sown in a field trial with two replicated and randomized blocks. Forage in 1.5 m × 9 m plots was grown in two consecutive years and cut four times per year (May-October). Analyses of variance were performed. In most herbages, α-tocopherol and β-carotene were positively correlated as were β-carotene and lutein; all vitamins were negatively correlated with fiber content and herbage yield. β-Carotene was positively correlated with protein content. α-Tocopherol and β-carotene contents were generally highest in October and lowest in July. Our results showed similar interrelationships in most investigated species, and we suggest that these species may be mixed when designing novel biodiverse mixtures for particular product quality characteristics. PMID:25573460

  7. Re-visiting the nutrition of dairy sheep grazing Mediterranean pastures

    Directory of Open Access Journals (Sweden)

    M. Decandia

    2010-04-01

    Full Text Available In the light of recent findings in sheep nutrition and behaviour, the diets of grazing dairy sheep should be based on forages encompassing a variety of complementary nutritional values and containing moderate levels of complementary plant secondary metabolites, until recently regarded as "anti-nutritional". In lactating sheep, pastures of tannin-containing legumes like sulla (Hedysarum coronarium and chicory (Cichorium intybus can be integrated with annual grasses for establishing sustainable artificial pastures under rainfed conditions. Diets based on these forages, while ensuring high milking performance, can mitigate the unbalance of CP to energy ratio of grazing sheep. By grazing sulla and annual or Italian ryegrass (50:50 by area as spatially conterminal monocultures or in timely sequence (complementary grazing sheep eat more and perform better than by grazing the ryegrass pasture only. Concentrate supplementation of lactating sheep should be preferably based on fibrous sources (soyhulls or beet pulps, particularly from mid-lactation onwards and when supplementation levels are high. Milk urea concentration is confirmedly a useful monitoring tool to balance protein nutrition and curb the waste of N at animal and system level.

  8. Comprehensive signal assignment of 13C-labeled lignocellulose using multidimensional solution NMR and 13C chemical shift comparison with solid-state NMR.

    Science.gov (United States)

    Komatsu, Takanori; Kikuchi, Jun

    2013-09-17

    A multidimensional solution NMR method has been developed using various pulse programs including HCCH-COSY and (13)C-HSQC-NOESY for the structural characterization of commercially available (13)C labeled lignocellulose from potatoes (Solanum tuberosum L.), chicory (Cichorium intybus), and corn (Zea mays). This new method allowed for 119 of the signals in the (13)C-HSQC spectrum of lignocelluloses to be assigned and was successfully used to characterize the structures of lignocellulose samples from three plants in terms of their xylan and xyloglucan structures, which are the major hemicelluloses in angiosperm. Furthermore, this new method provided greater insight into fine structures of lignin by providing a high resolution to the aromatic signals of the β-aryl ether and resinol moieties, as well as the diastereomeric signals of the β-aryl ether. Finally, the (13)C chemical shifts assigned in this study were compared with those from solid-state NMR and indicated the presence of heterogeneous dynamics in the polysaccharides where rigid cellulose and mobile hemicelluloses moieties existed together. PMID:24010724

  9. In situ biomonitoring of the genotoxic effects of mixed industrial emissions using the Tradescantia micronucleus and pollen abortion tests with wild life plants: Demonstration of the efficacy of emission controls in an eastern European city

    Energy Technology Data Exchange (ETDEWEB)

    Misik, Miroslav [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Micieta, Karol [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Solenska, Martina [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Misikova, Katarina [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Pisarcikova, Helena [Department of Botany, Comenius University in Bratislava, Faculty of Natural Sciences, Revova 39, SK 811 02 Bratislava 1 (Slovakia); Knasmueller, Siegfried [Institute of Cancer Research, Department of Inner Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna (Austria)]. E-mail: siegfried.knasmueller@meduniwien.ac.at

    2007-01-15

    Aim of the study was to monitor changes of genotoxic activity of urban air caused by an incinerator and a petrochemical plant in Tradescantia micronucleus (Trad-MCN) and pollen fertility assays with wild plants (Chelidonium majus, Clematis vitalba, Cichorium intybus, Linaria vulgaris, Robinia pseudoacacia). While in the first sampling period (1997-2000) significantly (on average 80%) more MN were found at the polluted site in comparison to controls from a rural area, no significant effects were observed during a later period (between 2003 and 2005). A similar pattern was observed in the pollen abortion assays in which the most pronounced effects were found in chicory and false acacia. The differences of the results obtained in the two periods can be explained by a substantial reduction of air pollution by use of new technologies. In particular the decrease of SO{sub 2} emissions may account for the effects seen in the present study. - Air pollution caused by industrial emissions induced micronuclei in Tradescantia and increased pollen abortion in wild plant species.

  10. Genomics of Compositae crops: reference transcriptome assemblies and evidence of hybridization with wild relatives.

    Science.gov (United States)

    Hodgins, Kathryn A; Lai, Zhao; Oliveira, Luiz O; Still, David W; Scascitelli, Moira; Barker, Michael S; Kane, Nolan C; Dempewolf, Hannes; Kozik, Alex; Kesseli, Richard V; Burke, John M; Michelmore, Richard W; Rieseberg, Loren H

    2014-01-01

    Although the Compositae harbours only two major food crops, sunflower and lettuce, many other species in this family are utilized by humans and have experienced various levels of domestication. Here, we have used next-generation sequencing technology to develop 15 reference transcriptome assemblies for Compositae crops or their wild relatives. These data allow us to gain insight into the evolutionary and genomic consequences of plant domestication. Specifically, we performed Illumina sequencing of Cichorium endivia, Cichorium intybus, Echinacea angustifolia, Iva annua, Helianthus tuberosus, Dahlia hybrida, Leontodon taraxacoides and Glebionis segetum, as well 454 sequencing of Guizotia scabra, Stevia rebaudiana, Parthenium argentatum and Smallanthus sonchifolius. Illumina reads were assembled using Trinity, and 454 reads were assembled using MIRA and CAP3. We evaluated the coverage of the transcriptomes using BLASTX analysis of a set of ultra-conserved orthologs (UCOs) and recovered most of these genes (88-98%). We found a correlation between contig length and read length for the 454 assemblies, and greater contig lengths for the 454 compared with the Illumina assemblies. This suggests that longer reads can aid in the assembly of more complete transcripts. Finally, we compared the divergence of orthologs at synonymous sites (Ks) between Compositae crops and their wild relatives and found greater divergence when the progenitors were self-incompatible. We also found greater divergence between pairs of taxa that had some evidence of postzygotic isolation. For several more distantly related congeners, such as chicory and endive, we identified a signature of introgression in the distribution of Ks values. PMID:24103297

  11. Drought resistance evaluation of four seedling chicory%4份菊苣种质材料苗期抗旱性评价

    Institute of Scientific and Technical Information of China (English)

    刘建宁; 石永红; 侯志宏; 王运琦; 李鹏; 邢亚亮; 吴欣明; 张燕

    2012-01-01

    采取温室盆栽干旱胁迫方法,研究不同程度干旱胁迫对4份菊苣材料苗期11种抗旱性指标的影响.结果表明,随着干旱胁迫程度加重,菊苣叶片含水量及相对含水量、株高、生物量降低,叶片相对电导率、可溶性糖含量、游离脯氨酸、根冠比变化趋势比较明显,株高胁迫指数与干物质胁迫指数变化不大;综合分析认为叶片含水量、相对含水量、株高、根冠比、细胞膜透性、可溶性糖含量可以作为菊苣抗旱性评价的主要指标,地上生物量、地下生物量、株高胁迫指数、干物质胁迫指数、脯氨酸仅可作为菊苣抗旱性评价的参考指标.通过对11种抗旱性指标进行隶属函数法综合评价,得出4份菊苣材料抗旱性由强到弱的顺序为:C2>C1>C4>C3,该结果与田间观测结果相一致.%The effects of drought stresses on eleven drought-resistant characteristics of four seedling chicory were studied by reducing soil water in a pot experiment.The leaf water content (LWC),leaf relative water content (LRWC),height and biomass of the four seedling chicory decreased,while their leaf soluble sugar content (LSSC),leaf relative electrical conductivity (LREC),proline and root/shoot increased with improved drought stress resistance.Drought stress did not significantly affect height stress index or dry matter stress index of four seedling chicory.It is concluded that LWC,LRWC,height,root-shoot ratio,LREC and LSSC can be used as the main evaluation index,while aboveground biomass,underground biomass,plant height stress index,dry matter stress index and proline can only be used as reference indexes for drought resistance evaluation of chicory.The capacity of drought resistance by subordinate function analysis of the four chicories was gauged as: C2>C1>C4>C3.This accorded with their practical performance.

  12. In vitro fermentation characteristics of novel fibers, coconut endosperm fiber and chicory pulp, using canine fecal inoculum.

    Science.gov (United States)

    de Godoy, M R C; Mitsuhashi, Y; Bauer, L L; Fahey, G C; Buff, P R; Swanson, K S

    2015-01-01

    The objective of this experiment was to determine the effects of in vitro fermentation of coconut endosperm fiber (CEF), chicory pulp (CHP), and selective blends of these substrates on SCFA production and changes in microbiota using canine fecal inocula. A total of 6 individual substrates, including short-chain fructooligosaccharide (scFOS; a well-established prebiotic source), pectin (PEC; used as a positive control), pelletized cellulose (PC; used as a negative control), beet pulp (BP; considered the gold standard fiber source in pet foods), CEF, and CHP, and 3 CEF:CHP blends (75:25% CEF:CHP [B1], 50:50% CEF:CHP [B2], and 25:75% CEF:CHP [B3]) were tested. Triplicate samples of each substrate were fermented for 0, 8, and 16 h after inoculation. A significant substrate × time interaction (P < 0.05) was observed for pH change and acetate, propionate, butyrate, and total SCFA concentrations. After 8 and 16 h, pH change was greatest for scFOS (-2.0 and -3.0, respectively) and smallest for PC (0.0 and -0.1, respectively). After 16 h, CEF had a greater butyrate concentration than CHP and all the CEF:CHP blends and it was not different than PEC. The substrate × time interaction was significant for bifidobacteria (P < 0.05) and lactobacilli (P < 0.05). After 8 h, bifidobacteria was greatest for BP and lowest for PC (12.7 and 10.0 log10 cfu/tube, respectively). After 16 h, PC had the lowest and scFOS had the greatest bifidobacteria (6.7 and 13.3 log10 cfu/tube, respectively). In general, CEF, CHP, and their blends had similar bifidobacteria populations after 8 and 16 h of fermentation when compared with BP and scFOS. After 16 h, lactobacilli populations were greatest for B1, B2, B3, BP, and scFOS, intermediate for PEC, and lowest for PC (P < 0.05). Overall, our data suggest that CEF had a butyrogenic effect and that CEF, CHP, and their blends had similar bifidobacteria and lactobacilli populations as popular prebiotic and fiber substrates. Future research should

  13. Produção do taro 'Chinês', em cultivo solteiro e consorciado com chicória Yield of 'Chinês' taro in monocrop system and intercropped with chicory

    Directory of Open Access Journals (Sweden)

    Néstor Antonio Heredia Zárate

    2007-10-01

    Full Text Available Objetiva-se com este trabalho avaliar a produtividade e a renda bruta do taro 'Chinês', conduzido em cultivo solteiro ou consorciado com chicória 'Escarola Lisa', nas condições ambientes de Dourados-MS. Os tratamentos foram arranjados no delineamento experimental de blocos casualizados, com cinco repetições. A colheita da chicória foi aos 97 dias após a semeadura e a do taro aos 203 dias. O consórcio foi avaliado pela razão de área equivalente (RAE e sua validação pela renda bruta. As plantas de taro e de chicória foram influenciadas significativamente pela consorciação, exceto para altura das plantas de chicória que foram semelhantes. O cultivo solteiro proporcionou maiores produções de taro (9,90; 3,96; 10,42 e 9,03 t ha-1 de folhas, rizomas-mãe, rizomas-filho comerciais e rizomas-filho não comerciais, respectivamente e diâmetro (19,22 cm e número (50,69 mil ha-1 de cabeças comerciais de chicória. A RAE para o consórcio taro-chicória foi de 0,92 e, por isso, o consórcio foi considerado como não efetivo.The present work had the aim of evaluating the yield and gross income of 'Chinês' taro that was carried out in monocrop and intercropped system with 'Escarola Lisa' chicory in environment conditions in Dourados-MS. Treatments were arranged in a randomized experimental block design with five replications. Chicory harvest was done at 97 days after sowing and taro harvest at 203 days. Intercropping was evaluated by Land Equivalent Ratio (LER and its validation was done determining gross income. Plants of taro and chicory were significantly influenced by means of cultivation, except to height of chicory plants that were similar. Monocrop system induced the highest yields of taro (9.90; 3.96; 10.42 and 9.03 t ha-1 of leaves, corms, commercial and non-commercial cormels, respectively and greatest diameter (19.22 cm and number (50.69 thousand ha-1 of commercial heads of chicory. LER for taro/chicory intercrop was 0.92 and

  14. Tolerance to salinity of chicory plants grown in hydroponics Tolerância do almeirão à salinidade em cultivo hidropônico

    OpenAIRE

    Jerônimo Luiz Andriolo; José Carlos Cazarotto Madalóz; Rodrigo dos Santos Godoi; Djeimi Isabel Janisch; Cláudia Alessandra Peixoto de Barros

    2008-01-01

    Chicory plants, cv. 'Amarelo' and 'Pão de Açúcar', were grown hydroponically under four NaCl concentrations in the nutrient solution to determine its tolerance to salinity. Sowing was made in rows placed 0.05m apart, on a 0.15m deep sand growing bed placed over fibber cement tiles. After emergence, plants were thinned to a plant density of 1,600plants m-2. A standard nutrient solution was used, with the main composition of, in mmol L-1, 13.5 NO3-; 2.5 NH4+; 1.5 H2PO4-; 1.5 SO4--; 7.5 Ca++; 10...

  15. A diet containing dried chicory root does not protect against post-weaning diarrhoea in an E. coli challenge model using piglets weaned at 7 weeks of age

    DEFF Research Database (Denmark)

    Hedemann, Mette Skou; Bach Knudsen, Knud Erik

    2010-01-01

    This study aimed to investigate whether fructan from dried chicory root provided protection against E. coli induced post-weaning diarrhoea in an experimental challenge model. Piglets from 6 litters, 8 piglets per litter, were included in the experiment. Starting 10 d prior to weaning, 4 piglets per...... litter received a control diet (0.9% fructan of DM) and 4 piglets received an experimental diet containing 20% dried chicory root (11.6% fructan of DM). The piglets were weaned at 7 weeks of age. On d 2 and 3 post-weaning half of the piglets were orally challenged with E. coli O149, the remaining piglets...... 4 and 5. Body weight gain and feed intake was not influenced by either E. coli challenge or diet. However, feed conversion ratio was lower in pigs fed the chicory diet during d 0-12 post-weaning. This experiment did not provide evidence for a protective effect of fructan on post-weaning diarrhoea...

  16. Dicty_cDB: Contig-U05566-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Contig-U05566-1 no gap 562 2 1380677 1380114 MINUS 1 1 U05566 0 0 0 0 0 0 1 0 0 0 0 0 0 0 Show C ... nces: 6905 Number of extensions: 18179 Number of succe ssful extensions: 1235 Number of sequences better t ... LMS) chicory Cichorium in... 36 1.0 2 ( EL371607 ) CCE L13625.b1_B23.ab1 CCE (LMS) endive Cichorium en... 3 ... (LMS) chicory Cichorium i... 36 1.0 2 ( EL343029 ) CCE L11023.b1_M20.ab1 CCE (LMS) endive Cichorium en... 3 ... 629,563 Number of extensions: 45531301 Number of succe ssful extensions: 3550224 Number of sequences bette ...

  17. Fructans of chicory

    DEFF Research Database (Denmark)

    Rumessen, J J; Gudmand-Høyer, E

    1998-01-01

    Fructans (fructooligosaccharides and inulin) are of increasing interest to clinical nutritionists as functional food additives. The chemically closely related food carbohydrates fructose and sorbitol are implicated in functional bowel disease. Intestinal handling of these carbohydrates is incompl......Fructans (fructooligosaccharides and inulin) are of increasing interest to clinical nutritionists as functional food additives. The chemically closely related food carbohydrates fructose and sorbitol are implicated in functional bowel disease. Intestinal handling of these carbohydrates...

  18. Effect of Chicory on Uric Acid and Uricopoiesis Metabolic Enzymes Activities of Hyperuricemia Quail%菊苣干预高尿酸血症鹌鹑尿酸及相关代谢酶活性研究

    Institute of Scientific and Technical Information of China (English)

    黄胜男; 林志健; 张冰; 赓迪; 牛红娟; 朱春胜; 王雪洁; 孙博喻

    2015-01-01

    目的:探讨菊苣对高尿酸血症(HUA)鹌鹑尿酸及相关代谢酶活性的影响,阐释菊苣防治HUA的可能机制。方法将60只鹌鹑,按体质量随机分为5组,即正常组,模型组,苯溴马隆组(20 mg·kg-1·d-1),菊苣高、低剂量组(10,5 g·kg-1·d-1),每组12只。除正常组喂饲鹌鹑普通饲料外,其余各组均给予高嘌呤饲料(普通饲料拌入酵母干粉15 g·kg-1·d-1)复制HUA模型。以菊苣治疗28 d,动态观察鹌鹑尿酸(uric acid,UA)水平及UA生成代谢酶活性的变化。结果给药7,14,21,28 d,菊苣高、低剂量组可显著降低鹌鹑UA水平(P<0.05);不同程度地抑制UA生成代谢酶5'-核苷酸酶(5'-NT)、腺苷脱氨酶(ADA)、嘌呤核苷磷酸化酶(PNP)、鸟嘌呤脱氨酶(GD)、黄嘌呤氧化酶(XO)的活性(P<0.05,P<0.01)。结论菊苣可有效降低鹌鹑HUA模型UA水平,可能与其降低UA代谢酶5'-NT、ADA、PNP、GD、XO的活性有关。%Objective To investigate the effect of chicory on uric acid and uricopoiesis metabolic enzymes activities in hyperuricemia quail and to explore the possible mechanism of chicory in preventing and treating hyperuricemia. Methods Sixty quails were evenly randomized into 5 groups according to the body weight, namely normal group, model group, benzbromarone(20 mg·kg-1·d-1) group, high- and low-dosage chicory groups(10, 5 g·kg-1·d-1 respectively). Except for the normal group, the quails in other groups were given high purine diet(ordinary forage mixed with 15 g·kg-1·d-1 of yeast powder) to induce hyperuricemia(HUA)model. And then we observed the changes of serum uric acid level and activities of uricopoiesis metabolic enzymes dynamically during the chicory treatment for 28 days. Results On the chicory administration day 7, 14, 21 and 28, serum uric acid level of quail was decreased obviously, and the activities of uricopoiesis metabolic enzymes of 5

  19. Determinação da difusividade efetiva de raiz de chicória Determination of effective diffusivity of chicory root

    Directory of Open Access Journals (Sweden)

    Rafael A. de Oliveira

    2006-04-01

    Full Text Available A inulina é um frutooligossacarídeo encontrado em diversos produtos agrícolas, dentre os quais o alho, a banana, a alcachofra de Jerusalém e a raiz de chicória. A inulina é geralmente utilizada em países desenvolvidos, como substituto do açúcar e/ou gordura devido as suas características que a enquadram como alimento funcional e dietético. A raiz de chicória é utilizada como fonte e matéria-prima para a extração comercial de inulina. Os experimentos constituíram-se na secagem das raízes fatiadas em um secador convectivo com fluxo do ar perpendicular, com base em um planejamento fatorial. Pôde-se determinar a difusividade efetiva (variável dependente para cada uma das combinações das variáveis independentes (temperatura e velocidade do ar, sendo as curvas desses resultados ajustadas pela solução da Segunda Lei de Fick e pelo modelo de Page. A difusividade efetiva variou de 3,51 x 10-10 m² s-1 até 10,36 x 10-10 m² s-1. Concluiu-se que, para a região de valores estudada, somente a temperatura do ar é estatisticamente significativa. Obteve-se, assim, modelo matemático de primeira ordem, representando o comportamento da difusividade efetiva em função da temperatura do ar. A melhor condição de secagem obtida foi a que utiliza a maior temperatura de ar de secagem.Inulin is a fructooligosacharide found in diverse agricultural products, amongst them garlic, banana, Jerusalem artichoke and chicory root. Inulin generally is used in developed countries, as a substitute of sugar and/or fat due to its characteristics of fitting as functional and dietary food. Chicory root is usually used as source and raw material for commercial extration of inulin. The experiments consisted on drying sliced chicory roots based on a factorial experimental design in a convective dryer whose alows the air to pass perpendicularly through the tray. Effective diffusivity (dependent variable has been determined for each experimental combination of

  20. Technology of Extraction and Purification of Inulin from Fresh Chicory Root%新鲜菊苣提取和纯化菊粉的工艺

    Institute of Scientific and Technical Information of China (English)

    柳溪; 王晓蕾

    2013-01-01

    The method of determination of insulin content using colorimeter wan studied , and the optimal condition of extracting inulin from fresh chicory roots with hot water and removing protein and colour was studied and selected by orthogonal design. The results showed the optimum process conditions of inulin extracting were as follow:solid-liquid ratio was 1∶1, immersion time was 40 min, heating extraction temperature was 100℃and time was 40 min, the extraction rate of inulin from chicory root could achieved 14.74 %; the optimum proportion of active carbon and inulin on removing protein was1 ∶ 1.5;the optimal removing colour condition were as follow:temperature was 70℃,time was 50 min, quantity of active carbon was 30 g/L, and decolourizing rate reached 74.36%.%  研究比色法测定菊粉含量的方法,并采用正交设计优选了从新鲜菊苣根中热水浸提菊粉的提取工艺条件和脱蛋白以及脱色的最佳条件。实验结果表明,菊粉提取的最佳工艺条件为:料液比1∶1,浸泡时间40 min,加热浸提温度为100℃,加热浸提时间40 min,菊粉的提取率达14.74%;脱蛋白时菊液与活性炭的最佳比例为1∶1.5;提取液最佳脱色工艺条件为:脱色温度70℃,脱色时间50 min,活性碳用量为30 g/L,脱色率为74.36%。

  1. Fatty acid composition of forage herb species

    DEFF Research Database (Denmark)

    Warner, D.; Jensen, Søren Krogh; Cone, J.W.;

    2010-01-01

    The use of alternative forage species in grasslands for intensive livestock production is receiving renewed attention. Data on fatty acid composition of herbs are scarce, so four herbs (Plantago lanceolata, Achillea millefolium, Cichorium intybus, Pastinaca sativa) and one grass species (timothy,...

  2. Vermischte Notizen zur Benennung hessischer Pflanzen : dritter Nachtrag zum "Namensverzeichnis zur Flora der Farn- und Samenpflanzen Hessens"

    OpenAIRE

    Buttler, Karl Peter

    1998-01-01

    Die seit dem zweiten Nachtrag (Januar 1997) bekannt gewordenen Ergänzungen und Verbesserungen zum "Namensverzeichnis" sind im dritten Nachtrag zusammengefaßt. Angefügt sind Anmerkungen zur Nomenklatur von Armeria vulgaris/A. elongata, Cichorium intybus, Pyrus pyraster, Silene dioica und S. latifolia subsp. alba, Silene viscaria, Stellaria palustris. Neue Kombination: Bassia scoparia var. subvillosa (Moquin-Tandon) Buttler.

  3. Fruit Flies Of The Genus Campiglossa (Diptera, Tephritidae) In Iran, With The Key To Species

    OpenAIRE

    Namin S. Mohamadzade; Nozari J.

    2015-01-01

    Seven species of Campiglossa occurring in Iran are reviewed, Campiglossa grandinata (Rondani) is recorded for the first time from Iran (Fars Province) and Cichorium intybus L. (Asteraceae) recorded as a new host plant for Campiglossa producta. A key to species is provided.

  4. AGROBACTERIUM-MEDIATED TRANSFORMATION OF СOMPOSITAE PLANTS. I. CONSTRUCTION OF TRANSGENIC PLANTS AND «HAIRY» ROOTS WITH NEW PROPERTIES

    OpenAIRE

    Matvieieva, N.

    2015-01-01

    The review explores some of the recent advances and the author's own researchs concerning biotechnological approaches for Agrobacterium tumefaciens and A. rhizogenes -mediated transformation of Compositae family plants. This paper reviews the results of genetic transformation of Compositae plants, including edible ( Cichorium intybus, Lactuca sativa ), oil ( Helianthus annuus ), decorative ( Gerbera hyb rida ), medical ( Bidens pilosa, Artemisia annua, Artemisia vulgaris, Calendula officinali...

  5. Fruit Flies Of The Genus Campiglossa (Diptera, Tephritidae In Iran, With The Key To Species

    Directory of Open Access Journals (Sweden)

    Namin S. Mohamadzade

    2015-06-01

    Full Text Available Seven species of Campiglossa occurring in Iran are reviewed, Campiglossa grandinata (Rondani is recorded for the first time from Iran (Fars Province and Cichorium intybus L. (Asteraceae recorded as a new host plant for Campiglossa producta. A key to species is provided.

  6. Impact of Variety and Agronomic Factors on Crude Protein and Total Lysine in Chicory; N(ε)-Carboxymethyl-lysine-Forming Potential during Drying and Roasting.

    Science.gov (United States)

    Loaëc, Grégory; Niquet-Léridon, Céline; Henry, Nicolas; Jacolot, Philippe; Jouquand, Céline; Janssens, Myriam; Hance, Philippe; Cadalen, Thierry; Hilbert, Jean-Louis; Desprez, Bruno; Tessier, Frédéric J

    2015-12-01

    During the heat treatment of coffee and its substitutes some compounds potentially deleterious to health are synthesized by the Maillard reaction. Among these, N(ε)-carboxymethyl-lysine (CML) was detected at high levels in coffee substitutes. The objective of this study was to evaluate the impact of changes in agricultural practice on the lysine content present in chicory roots and try to limit CML formation during roasting. Of the 24 varieties analyzed, small variations in lysine content were observed, 213 ± 8 mg/100 g dry matter (DM). The formation of lysine tested in five commercial varieties was affected by the nitrogen treatment with mean levels of 176 ± 2 mg/100 g DM when no fertilizer was added and 217 ± 7 mg/100 g DM with a nitrogen supply of 120 kg/ha. The lysine content of fresh roots was significantly correlated to the concentration of CML formed in roasted roots (r = 0.51; p < 0.0001; n = 76). PMID:26548778

  7. Effect of Anti-cerebral Ischemia of Gross Saponin of Chicory%奇可力皂苷抗脑缺血作用研究

    Institute of Scientific and Technical Information of China (English)

    李晶; 昌友权; 曲红光; 尹尔丽; 昌喜涛

    2005-01-01

    目的:探讨奇可力皂苷(Gross saponin of Chicory,GSC)的抗脑缺血作用.方法:以电阻法阻断大脑一侧中动脉造成局灶性脑缺血模型,观察奇可力皂苷对脑梗死面积及行为评分的影响以探讨其抗脑缺血作用及其作用机理;采用大鼠血瘀模型,观察奇可力皂苷对血瘀大鼠血小板聚集率及凝血时间的影响.结果:GSC10.4、20.8、31.2mg/kg均可明显减少脑梗死面积(p<0.05,p<0.01);GSC10.4、20.8、31.2mg/kg可明显抑制血瘀大鼠的血小板聚集率(p<0.05),还可明显延长血瘀大鼠的凝血时间(p<0.05).结论:GSC具有一定的抗脑缺血作用,其作用机理可能与降低血小板聚集性及抗凝血作用相关.

  8. ALLELOPATHIC POTENTIAL OF Portulaca oleracea L. SEED EXTRACTS ON GERMINATION AND SEEDLING GROWTH OF Cichorium endivia L., Lactua sativa L., Echinochloa crus-galli L., AND Brassica tournefortii Gouan

    Directory of Open Access Journals (Sweden)

    Hanaa Fahmy Shehata

    2014-08-01

    Full Text Available Present study was formulated to find out the phytotoxic effects of different concentrations of water, ethyl acetate, petroleum ether and methanol extracts of Portulaca oleracea seeds on germination, germination index and seedlings growth of Cichorium endivia, Lactuca sativa Echinochoa crus-galli, and Brassica tournefortii. Also the total phenolics and flavonoids were determined. Results indicated that the responses of allelopathic effects were depends on extract type and concentration. Furthermore, the higher concentration had a stronger inhibitory effect on seed germination whereas in some cases the lower concentration showed a stimulatory effect. Hence, it is suggested that Portulaca oleracea seeds has strong allelopathic potential and might be use for biological control of weeds.

  9. Impact of Cultivation Modes of Puna Chicory on its Agronomic Traits and Yield%不同栽培模式对黔引普那菊苣农艺性状及产量的影响

    Institute of Scientific and Technical Information of China (English)

    陈志祯; 何胜礼; 黄河斋; 陈克庆; 陈进碧; 高巍

    2012-01-01

    采取撒播、条播、穴播和育苗移栽4种栽培模式种植黔引普那菊苣,观测其生育期、再生速度、产草量.结果表明:黔引普那菊苣在黔南温热地区4种栽培模式上均能很好地完成生育期,不同栽培模式之间生育天数、生长天数以及再生速度差异不显著(P>0.05).4种栽培模式以穴播产量最高,撒播次之,育苗移栽最低,条播、撒播、穴播分别比育苗移栽提高8194、9134和10146kg/hm2,差异显著(P<0.05).建议田间规模种植时采取穴播.%The agronomic traits and yields of puna chicory under four cultivation modes (taking sowing, drilling, dibbling and transplanting seedling )were compared by observing their growth period, regeneration rate and grass yield in warm area of Qian-nan Prefecture. The results showed that the puna chicory completed the reproductive phase of growth well under four cultivation modes, there were no significant differeneesC/MJ.OS) in the fertility day, growth day and regeneration speed of puna chicory un-der the cultivation modes. In the four cultivation modes, the yield in dibble mode was the highest, and the yield in the trans-planting seedling was the lowest. Compared with the transplanting seedling, the yields in drilling mode, sowing mode and dib-bling mode were higher by 8 194 kg/hm2, 9 134 kg/hm2 and 10 146 kg/hm2 respectively. So the dibbling mode was the best for puna chicory plant.

  10. Study on production process of low-fat chicory dietary fiber set yogurt%低脂菊苣膳食纤维凝固型酸奶的生产工艺

    Institute of Scientific and Technical Information of China (English)

    云月英; 韩铖铖; 王文龙

    2011-01-01

    以菊苣膳食纤维和脱脂乳粉为原料,通过乳酸菌发酵生产一种低脂凝固型酸奶.通过单因素试验和均匀设计试验,初步确定最佳发酵工艺条件为:菊苣膳食纤维2.08%,脱脂乳粉12.7%,蔗糖7.06%,发酵剂接种量为0.8 g/L,发酵温度为42.8℃,发酵时间为7.5 h.%A low-fit set yoghurt was prepared with mixture of chicory dietary fiber and skimmed milk powder and by fermentation with Lac-tobacillus starter. The optimum fermentation conditions were determined by single factor and uniform design, which were as follows: chicory dietary fiber 2.08%, skimmed milk powder 12.7%, sugar 7.06%, Lactobacillus starter inoculum by 5%, fermentation under 42.8 ℃ for 7.5 h.

  11. Effect of seed mixture composition and management on competitiveness of herbs in temporary grasslands

    DEFF Research Database (Denmark)

    Mortensen, Tine Bloch; Søegaard, Karen; Eriksen, Jørgen

    2012-01-01

    perennial ryegrass (Lolium perenne) and white clover (Trifolium repens), 2) cutting frequency, and 3) slurry application. The experiment was carried out over three years. The herb mixture contained salad burnet (Sanguisorba minor), fenugreek (Trigonella foenum-graecum), chicory (Cichcorium intybus), caraway...

  12. Deep rooting plants influence on soil hydraulic properties and air conductivity over time

    Science.gov (United States)

    Uteau, Daniel; Peth, Stephan; Diercks, Charlotte; Pagenkemper, Sebastian; Horn, Rainer

    2014-05-01

    Crop sequences are commonly suggested as an alternative to improve subsoil structure. A well structured soil can be characterized by enhanced transport properties. Our main hypothesis was, that different root systems can modify the soil's macro/mesopore network if enough cultivation time is given. We analyzed the influence of three crops with either shallower roots (Festuca arundinacea, fescue) or taproots (Cichorium intybus, chicory and Medicago sativa, alfalfa). The crops where cultivated on a Haplic Luvisol near Bonn (Germany) for one, two or three years. Undisturbed soil cores were taken for measurement of unsaturated hydraulic conductivity and air permeability. The unsaturated conductivity was measured using the evaporation method, monitoring the water content and tension at two depths of each undisturbed soil core. The van Genuchten-Mualem model (1991) was fitted to the measured data. Air permeability was measured in a permeameter with constant flow at low pressure gradient. The measurements were repeated at -1, -3, -6, -15, -30 and -50 kPa matric tension and the model of Ball et al. (1988) was used to describe permeability as function of matric tension. Furthermore, the cores equilibrated at -15 kPa matric tension were scanned with X-Ray computer tomography. By means of 3D image analysis, geometrical features as pore size distribution, tortuosity and connectivity of the pore network was analyzed. The measurements showed an increased unsaturated hydraulic conductivity associated to coarser pores at the taprooted cultivations. A enhanced pore system (related to shrink-swell processes) under alfalfa was observed in both transport measurements and was confirmed by the 3D image analysis. This highly functional pore system (consisting mainly of root paths, earthworm channels and shrinking cracks) was clearly visible below the 75 cm of depth and differentiated significantly from the other two treatments only after three years of cultivation, which shows the time

  13. Effect of Chicoric Acid on Mast Cell-Mediated Allergic Inflammation in Vitro and in Vivo.

    Science.gov (United States)

    Lee, Na Young; Chung, Kyung-Sook; Jin, Jong Sik; Bang, Keuk Soo; Eom, Ye-Jin; Hong, Chul-Hee; Nugroho, Agung; Park, Hee-Jun; An, Hyo-Jin

    2015-12-24

    Chicoric acid (dicaffeoyl-tartaric acid), is a natural phenolic compound found in a number of plants, such as chicory (Cichorium intybus) and Echinacea (Echinacea purpurea), which possesses antioxidant, anti-inflammatory, antiviral, and analgesic activities. Although these biological effects of chicoric acid have been investigated, there are no reports of its antiallergic-related anti-inflammatory effects in human mast cells (HMC)-1 or anaphylactic activity in a mouse model. Therefore, we investigated the antiallergic-related anti-inflammatory effect of chicoric acid and its underlying mechanisms of action using phorbol-12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated HMC-1 cells. Chicoric acid decreased the mRNA expression of pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β. We studied the inhibitory effects of chicoric acid on the nuclear translocation of nuclear factor kappa B (NF-κB) and activation of caspase-1. However, mitogen-activated protein kinase (MAPK) activation was not sufficient to abrogate the stimulus. In addition, we investigated the ability of chicoric acid to inhibit compound 48/80-induced systemic anaphylaxis in vivo. Oral administration of chicoric acid at 20 mg/kg inhibited histamine release and protected mice against compound 48/80-induced anaphylactic mortality. These results suggest that chicoric acid has an antiallergic-related anti-inflammatory effect that involves modulating mast cell-mediated allergic responses. Therefore, chicoric acid could be an efficacious agent for allergy-related inflammatory disorders. PMID:26593037

  14. Molecular weight distribution of soluble fiber fractions and short chain fatty acids in ileal digesta of growing pigs.

    Science.gov (United States)

    Ivarsson, E; Andersson, R; Lindberg, J E

    2012-12-01

    The effect of dietary fiber source on molecular weight (MW) distribution of soluble fiber fractions and short chain fatty acids (SCFA) in ileal digesta of 7 post valve T-cecum (PVTC) cannulated growing pigs was studied. Pigs were fed semisynthetic diets with sugar beet (Beta vulgaris) pulp (SBP) or chicory (Cichorium intybus) forage (CFO) as fiber sources of which the soluble nonstarch polysaccharide (NSP) fraction originated mainly from pectin. Three MW intervals were selected-large MW (MWL): 10,000,000 to 1,000,000 g/mol, medium MW (MWM): 1,000,000 to 200,000 g/mol, and small MW (MWS): 200,000 to 10,000 g/mol-and the relative distribution (% of total) of molecules in each interval was calculated. The MWM fraction was higher (P < 0.05) in ileal digesta of pigs fed diet SBP and the MWS fraction was higher (P < 0.05) in ileal digesta of pigs fed diet CFO. The mole/100 mole of propionic acid (HPr) was higher (P < 0.010) in pigs fed diet SBP whereas pigs fed diet CFO had higher (P < 0.010) mole/100 mole of acetic acid (HAc). The proportion of the MWL and MWM fractions in ileal digesta were negatively correlated to HAc (r = -0.52, P = 0.05, and r = -0.62, P = 0.02, respectively). The proportion of MWM in ileal digesta was positively correlated to HPr (r = 0.83; P = 0.001) whereas MWS and HPr were negatively correlated (r = -0.76; P = 0.002). In conclusion, the bacterial degradation of the soluble NSP fraction is selective and MW distribution may explain differences in SCFA production. PMID:23365284

  15. Review of scientific evidence of medicinal convoy plants in traditional Persian medicine

    Directory of Open Access Journals (Sweden)

    Seyede Nargess Sadati

    2016-01-01

    Full Text Available One concept used in traditional Persian medicine (TPM for multidrug therapy is that of the convoy drug (Mobadregh. According to TPM texts, convoy drugs are substances (or drugs, which facilitate the access of drugs or foods to the whole body or to specific organs. This study reviewed some convoy drugs presented in TPM, their biological effects, and their probable interactions with main drugs, considering the increased absorption through inhibition of P-glycoprotein (P-gp efflux function, bioavailability-enhancing effects, and decreased metabolism of the main drug using electronic databases including PubMed, Scopus, ScienceDirect, and Google Scholar in November and December, 2013. Recent studies have proven the beneficial effects of Crocus sativus L. (saffron and camphor on the heart and brain, the cerebral therapeutic effects of Asarum europaeum (hazelwort, the hepatoprotective effects of Cichorium intybus (chicory, and Apium graveolens (celery seeds, and the diuretic effects of Cinnamomum zeylanicum (cinnamon, and Cucumis melo (melon seeds. The effects of vinegar in targeting the liver and brain have also been demonstrated. An evaluation of the results demonstrated that the suggested convoy drugs, including Piper nigrum (black pepper, Piper longum (long pepper, red wine, Camellia sinensis (tea, hazelwort, Mentha longifolia (pennyroyal, Anethum graveolens (dill, Foeniculum vulgare (fennel, cinnamon, and Sassafras albidum (sassafras can increase the bioavailability of coadministered drugs by inhibition of P-gp or cytochrome P450s (CYP450s or both of them. This evidence could be a good basis for the use of these agents as convoys in TPM.

  16. Review of Scientific Evidence of Medicinal Convoy Plants in Traditional Persian Medicine.

    Science.gov (United States)

    Sadati, Seyede Nargess; Ardekani, Mohammad Reza Shams; Ebadi, Nastaran; Yakhchali, Maryam; Dana, Azadeh Raees; Masoomi, Fatemeh; Khanavi, Mahnaz; Ramezany, Farid

    2016-01-01

    One concept used in traditional Persian medicine (TPM) for multidrug therapy is that of the convoy drug (Mobadregh). According to TPM texts, convoy drugs are substances (or drugs), which facilitate the access of drugs or foods to the whole body or to specific organs. This study reviewed some convoy drugs presented in TPM, their biological effects, and their probable interactions with main drugs, considering the increased absorption through inhibition of P-glycoprotein (P-gp) efflux function, bioavailability-enhancing effects, and decreased metabolism of the main drug using electronic databases including PubMed, Scopus, ScienceDirect, and Google Scholar in November and December, 2013. Recent studies have proven the beneficial effects of Crocus sativus L. (saffron) and camphor on the heart and brain, the cerebral therapeutic effects of Asarum europaeum (hazelwort), the hepatoprotective effects of Cichorium intybus (chicory), and Apium graveolens (celery) seeds, and the diuretic effects of Cinnamomum zeylanicum (cinnamon), and Cucumis melo (melon) seeds. The effects of vinegar in targeting the liver and brain have also been demonstrated. An evaluation of the results demonstrated that the suggested convoy drugs, including Piper nigrum (black pepper), Piper longum (long pepper), red wine, Camellia sinensis (tea), hazelwort, Mentha longifolia (pennyroyal), Anethum graveolens (dill), Foeniculum vulgare (fennel), cinnamon, and Sassafras albidum (sassafras) can increase the bioavailability of coadministered drugs by inhibition of P-gp or cytochrome P450s (CYP450s) or both of them. This evidence could be a good basis for the use of these agents as convoys in TPM. PMID:27041871

  17. [Molecular docking analysis of xanthine oxidase inhibition by constituents of cichory].

    Science.gov (United States)

    Wang, Xue-jie; Lin, Zhi-jian; Zhang, Bing; Zhu, Chun-sheng; Niu, Hong-juan; Zhou, Yue; Nie, An-zheng; Wang, Yu

    2015-10-01

    Human xanthine oxidase is considered to be a target for therapy of hyperuricemia. Cichorium intybus is a Chinese plant medicine which widely used in Xinjiang against various diseases. In order to screen the inhibitors of xanthine oxidase from C. intybus and to explore main pharmacological actions of cichory a compound collection of C. intybus was built via consulting related references about chemical research on cichory. The three-dimensional crystal structure of xanthine oxidase (PDB code: 1N5X) from Protein Data Bank was downloaded.. Autodock 4.2 was employed to screen the inhibitors of xanthine oxidase from cichory 70 compounds were found to possess quite low binding free energy comparing with TEI (febuxostat). C. intybus contains constituents possessing potential inhibitive activity against xanthine oxidase. It can explain the main pharmacological actions of cichory which can significantly lower the level of serum uric acid. PMID:26975108

  18. Tolerance to salinity of chicory plants grown in hydroponics Tolerância do almeirão à salinidade em cultivo hidropônico

    Directory of Open Access Journals (Sweden)

    Jerônimo Luiz Andriolo

    2008-08-01

    Full Text Available Chicory plants, cv. 'Amarelo' and 'Pão de Açúcar', were grown hydroponically under four NaCl concentrations in the nutrient solution to determine its tolerance to salinity. Sowing was made in rows placed 0.05m apart, on a 0.15m deep sand growing bed placed over fibber cement tiles. After emergence, plants were thinned to a plant density of 1,600plants m-2. A standard nutrient solution was used, with the main composition of, in mmol L-1, 13.5 NO3-; 2.5 NH4+; 1.5 H2PO4-; 1.5 SO4--; 7.5 Ca++; 10.0 K+ and 1.5 Mg++. The control (T1 was the standard nutrient solution, without addition of NaCl. Four salinity levels were compared, by adding 0.697 (T2, 1.627 (T3, 2.556 (T4 and 3.485 (T5g L-1 of NaCl. The nutrient solution was delivered to plants four times a day, in a closed system. A completely split plot randomised experimental design was used, with four replications. Plants were harvested at 31 days after sowing, by cutting the shoot at 0.02m height. Fresh and dry mass of both cultivars decreased linearly by effect of salinity. For similar values of electrical conductivity, the decrease in fresh mass was stronger in plants of the Amarelo cultivar. Adding NaCl to the nutrient solution is a technique that may be used to reduce the water content of chicory plants grown hydroponically.Quatro concentrações de NaCl na solução nutritiva foram empregadas para determinar a tolerância das cultivares de almeirão "Amarelo" e "Pão de Açúcar" à salinidade da solução nutritiva. A semeadura foi feita em fileiras distanciadas de 0,05m, em uma camada de areia de 0,15m de profundidade, distribuída sobre telhas de fibrocimento. Após a emergência foi efetuado o desbaste, mantendo-se uma densidade de 1.600plantas m-2. Foi empregada uma solução nutritiva com a composição de, em mmol L-1, 13,5 NO3-; 2,5 NH4+; 1,5 H2PO4-; 1,5 SO4--; 7,5 Ca++; 10,0 K+ e 1,5 Mg++. A testemunha (T1 foi a solução nutritiva descrita anteriormente, sem adição de Na

  19. Fatty acids, α-tocopherol, β-carotene, and lutein contents in forage legumes, forbs, and a grass-clover mixture.

    Science.gov (United States)

    Elgersma, Anjo; Søegaard, Karen; Jensen, Søren Krogh

    2013-12-11

    Fresh forages are an important natural source of vitamins and fatty acids in ruminant diets, and their concentrations in forage species are important for the quality of animal-derived foods such as dairy and meat products. The aims of this study were to obtain novel information on vitamins and fatty acids (FA) in a variety of forage legumes and non-legume forb species compared to a grass-clover mixture and to explore implications for animal-derived products. Seven dicotyledons [four forbs (salad burnet ( Sanguisorba minor ), caraway ( Carum carvi ), chicory ( Cichorium intybus ), and ribwort plantain ( Plantago lanceolata )) and three legume species (yellow sweet clover ( Melilotus officinalis ), lucerne ( Medicago sativa ), and birdsfoot trefoil ( Lotus corniculatus ))] and a perennial ryegrass-white clover mixture were investigated in a cutting trial with four harvests (May-October) during 2009 and 2010. The experimental design was a randomized complete block, and analyses of variance were performed. In addition, three other forbs were grown: borage ( Borago officinalis ), viper's bugloss ( Echium vulgare ), and chervil ( Anthriscus cerefolium ). Lucerne and yellow sweet clover had the lowest α-tocopherol concentrations (21-23 mg kg(-1) DM) and salad burnet and ribwort plantain the highest (77-85 mg kg(-1) DM); β-carotene concentrations were lowest in lucerne, salad burnet, and yellow sweet clover (26-33 mg kg(-1) DM) and highest in caraway, birdsfoot trefoil, and ribwort plantain (56-61 mg kg(-1) DM). Total FA concentrations were lowest in lucerne, ribwort plantain, chicory, and yellow sweet clover (15.9-19.3 g kg(-1) DM) and highest in caraway and birdsfoot trefoil (24.5-27.0 g kg(-1) DM). Birdsfoot trefoil had the highest (53.6 g 100 g(-1) FA) and caraway and lucerne the lowest (33.7-35.7 g 100 g(-1) FA) proportions of n-3 FA. This study demonstrated higher vitamin concentrations in some forbs compared with major forages such as lucerne and grass

  20. Na2CO3和NaHCO3胁迫对菊苣种子萌发的影响%Effects of Na2CO3 and NaHCO3 Stress on Seed Germination of Chicory

    Institute of Scientific and Technical Information of China (English)

    郭丹丹

    2013-01-01

    [目的]为研究Na2CO3和NaHCO3等碱性盐对植物的胁迫伤害提供依据.[方法]用不同浓度的Na2CO3和NaHCO3溶液处理菊苣种子,并对其发芽率、发芽势、根长、苗长和鲜重等生理指标进行研究.[结果]低浓度Na2CO3和NaHCO3对萌发后菊苣的苗长、根长和鲜重有一定的促进作用,但对根苗比的影响表现为抑制.[结论]低浓度Na2CO3和NaHCO3对种子萌发有增效效应.%[Objective] The research aimed to provide the basis for the study of the damage of the stress of basic salt such as Na2CO3 and NaHCO3 on the plants.[Method] Chicory seeds were treated with Na2CO3 and NaHCO3 solution of different concentration.The physiological indicators such as germination rate,germination potential,root length,seedling length and fresh weight were studied.[Result] A low concentration of Na2CO3 and NaHCO3 could promote germination rate,germination potential,then promote seedling length,root length and fresh weight after germination.However,it showed the performance for suppression on the ratio of root and shoot.[Conclusion] A low concentration of Na2CO3 and NaHCO3 had synergistic effect on chicory seed germination rate and germination potential.

  1. APLICACIÓN DE LA TECNICA DE IV GAMA PARA LA ELABORACIÓN DE ENSALADAS EVALUATION OF IV GAMA TECHNIQUE TO PREPARE VEGETABLE SALADS

    OpenAIRE

    Auris Damely García Méndez

    2008-01-01

    El propósito de la aplicación de la técnica de IV Gama en la elaboración de ensaladas listas para el consumo fresco, esta dirigido al control del deterioro en lechuga americana (Lactuca sativa), zanahoria (Daucus carota L.), radicchio (Cichorium intybus L.) y escarola (Cichorium endivia L.), destinadas a preparar ensaladas tipo “Mediterránea y Cheff”. La metodología permitió seleccionar y evaluar la técnica de IV Gama, así como las buenas prácticas de manufactura para extender la vida comerci...

  2. Aplicación de la técnica de iv gama para la elaboración de ensaladas.

    OpenAIRE

    García Méndez, Auris Damely

    2011-01-01

    El propósito de la aplicación de la técnica de IV Gama en la elaboración de ensaladas listas para el consumo fresco, está dirigido al control del deterioro en lechuga americana (Lactucasativa), zanahoria (Daucus carota L.), radicchio (Cichorium intybus L.) y escarola (Cichorium endivia L.), destinadas a preparar ensaladas tipo “Mediterránea y Cheff”. La metodología permitió seleccionar y evaluar la técnica de IV Gama, así como las buenas prácticas de manufactura para extender la vida comercia...

  3. 日粮中菊苣与饲料比例对鹅生长发育以及养殖效益的影响%Effects of Different Chicory and Formula Feed Ratios in Diet on Growth and Cultivation Benefit of Goose

    Institute of Scientific and Technical Information of China (English)

    王守红; 盖玉芳; 毕建花; 张家宏; 马谈斌; 韩光明; 寇祥明; 金银根; 唐鹤军

    2012-01-01

    This experiment was conducted to study the effects of different chicory and formula feed ratios in the diet on the growth and cultivation benefit of goose. The results indicated that: after six weeks' rearing of 21 -day -old goose, the weight gain rate of goose in the control group (chicory: formula feed =0: 1) was significantly higher than that in various test groups (P <0. 05) , while test group 1 (chicory: formula feed =4:1) and test group 2 (chicory: formula feed =8:1) had no significant difference in goose weight gain rate. Breast width, keel length and shank girth of goose in the control group were significantly higher than those in test group 2 (P < 0.05) , and other body - size indexes in each group was not significantly different. Gizzard index, total intestinal length, small intestine length and cecum length appeared significant difference among various groups (P <0.05) , and other gut indexes had no significant difference. The rearing economic benefit of test group 2 came to the highest among different groups. We suggested that the suitable chicory and formula feed ratio in the diet was between 4:1 and 8:1.%研究了日粮中菊苣鲜草与配合饲料的比例对鹅生长发育以及养殖经济效益的影响.结果显示:饲养21日龄鹅6周后,对照组(菊苣∶饲料=0∶1)鹅的增重率显著高于各试验组(P<0.05),试验组1(菊苣∶饲料=4∶1)与试验组2(菊苣∶饲料=8∶1)之间差异不显著;对照组的胸宽、龙骨长和胫围显著大于试验组2(P <0.05),各组之间其他体尺指标差异不显著;各组之间鹅的肌胃指数、总肠长、小肠长和盲肠长呈现显著差异(P<0.05),其他内脏指标差异不显著;试验组2的养殖经济效益最高.建议鹅日粮中菊苣与饲料的比例为(4~8)∶1.

  4. Produção de chicória em função do período de cobertura com tecido de polipropileno Chicory production affected by different periods of covering with polypropylene

    Directory of Open Access Journals (Sweden)

    Anderson Luiz Feltrim

    2006-06-01

    Full Text Available O experimento foi realizado na UNESP em Jaboticabal (SP com o objetivo de avaliar períodos de cobertura de plantas de chicória com polipropileno branco, de 20 g m-2, sobre a produção de chicória crespa. O delineamento experimental foi o de blocos casualizados, em esquema fatorial 4x2, com quatro repetições. Os tratamentos constituíram-se de dois genótipos de chicória (cv. Chicória Crespa, da Isla Sementes, e o genótipo 'AF-218', da Sakata Seeds e de quatro sistemas de cultivo: (a ausência de cobertura com polipropileno; (b cobertura pos 15 dias após o transplantio (DAT; (c cobertura aos 30 DAT; e (d 42 DAT coberto, o qual correspondeu a todo o período de crescimento da chicória no campo. A semeadura foi realizada em 02/06/2003 e o transplantio em 27/06/2003. A colocação do polipropileno foi feita diretamente sobre as plantas, fixando-se as laterais na parte externa do canteiro. Na colheita, avaliou-se a altura, número de folhas, diâmetro das plantas, massa fresca e seca da parte aérea. O genótipo 'AF-218' apresentou maior massa fresca da parte aérea que a cv. Chicória Crespa. À medida em que houve aumento do período de cobertura com polipropileno, observou-se aumento na altura, número de folhas e na massa fresca da parte aérea dos dois genótipos. O uso do polipropileno durante todo o ciclo (42 dias resultou em maior massa seca e promoveu precocidade da colheita em oito dias, além de qualidade superior, com folhas mais tenras e limpas.The experiment was carried out in Jaboticabal, São Paulo State, Brazil, to evaluate the production of chicory plants subjected to periods of covering with white polypropylene of 20 g m². The experimental design was a randomized block using a factorial scheme 4x2, with four replications. The treatments consisted of two chicory genotypes (cv. Chicória Crespa, of Isla Seeds, and 'AF-218', of Sakata Seeds and four cultivation systems: (a non covering of the plants with polypropylene; (b

  5. 菊苣化学成分的LC-MS/MS定性分析与HPLC含量测定%Qualitative and quantitative analysis of Chicory Root by LC/MS and HPLC

    Institute of Scientific and Technical Information of China (English)

    朱春胜; 林志健; 张冰; 牛红娟; 王雪洁; 张晓朦

    2016-01-01

    目的:建立菊苣化学成分的定性、定量分析方法,完善药材质量控制手段。方法以 HPLC-DAD-ESI-MS 方法,对菊苣化学成分进行定性鉴定,并采用 HPLC 方法同时测定菊苣中5种化学成分含量。结果从菊苣中共鉴定了10个化学成分,其中6个成分的保留时间和质谱碎片离子信息与对照品一致,分别为秦皮甲素、绿原酸、秦皮乙素、菊苣酸、异绿原酸 B、异绿原酸 A;根据质谱碎片信息并结合文献数据初步确定了另外4个成分的化学结构。建立了菊苣中绿原酸、秦皮乙素、菊苣酸、异绿原酸 B、异绿原酸 A 的回归方程,线性关系均良好(r >0.9995),加样回收率为95.94%~104.41%,采用此方法成功检测了10个菊苣样品中上述5种成分的含量。结论研究建立的方法可定性、定量分析菊苣中的主要化学成分,可用于菊苣药材的质量分析。%Objective To establish the methods for identification and determination of chemical components in Chicory (Herba Cichorii Radix Cichorii,Juju)to optimize the quality control methods of Chinese medicinal materials.Methods High performance liquid chromatography (HPLC)-diode array detctor (DAD)-electrospray ionization mass spectrometry (ESI-MS)was used for separation and charac-terization,and HPLC for simultaneous determination of five active components in Chicory.Results Six of ten components identified were aesculin,chlorogenic acid,esculetin,chicoric acid,chlorogenic acid B and chlorogenic acid A,in line with the features of retention time and MS fragment ions,four of ten were preliminarily determined their chemical constitutions by MS /MS spectra and data from literature.In addition,the regression equations of chlorogenic acid,esculetin,chicoric acid,chlorogenic acid B and chlorogenic acid A were established and showed good linear relationship(r >0.999 5)with the recovery of standard additon of 95.94% ~104.41%.The contents

  6. Application of Chicory Residue as a New Type of Biological Feed Additive in the Production of Laying Hens%菊苣残渣生物饲料添加剂在蛋鸡生产中的应用

    Institute of Scientific and Technical Information of China (English)

    王宁宁; 王勇; 周霖; 闫从阳

    2013-01-01

    [目的]研制1种新型的生物饲料添加剂,并探讨其对蛋鸡养殖的实际功效.[方法]选用枯草芽孢杆菌、嗜酸乳杆菌、保加利亚乳杆菌、地衣芽孢杆菌作为试验菌株,以菊苣残渣为发酵原料,研制1种新型的生物饲料添加剂,并探讨其对蛋鸡生产性能和鸡蛋品质的影响.[结果]在饲料中添加新型的生物饲料添加剂后,蛋鸡产蛋率提高2.3%,料蛋比降低13.9%,平均蛋重提高1.4%,蛋黄黄度明显提高15.5%.当该新型的生物饲料添加剂的添加量为1%时,蛋黄的粗蛋白含量也有显著提高.[结论]用菊苣研制成的新型生物饲料添加剂可用于蛋鸡生产中.%[Objective] The purpose of the research was to develop a new type of biological feed additive and discuss its actual efficiency on the culture of laying hens. [Method] Bacillus subtilis, Lactobacillus acidophilus, Lactobacillus delbrueckii and Bacillus licheniformis were used as test strains and chicory residue was used as fermentation material to develop a new type of biological feed additive. And its effects on the production performance of laying hens and egg quality were studied. [Result] After adding new type of biological feed additive in the feed, the egg production rate increased 2.3% , feed-egg ratio decreased 13.9% , average egg weight increased by 1.4% , the egg yolk yellow significantly improved 15.5%. [Conclusion] The new type of biological feed additive produced with chicory residue could be used in the production of laying hens.

  7. AGROBACTERIUM-MEDIATED TRANSFORMATION OF COMPOSITAE PLANTS. I. CONSTRUCTION OF TRANSGENIC PLANTS AND «HAIRY» ROOTS WITH NEW PROPERTIES

    Directory of Open Access Journals (Sweden)

    N. A.Matvieieva

    2013-02-01

    Full Text Available The review explores some of the recent advances and the author's own researchs concerning biotechnological approaches for Agrobacterium tumefaciens- and A. rhizogenes-mediated transformation of Compositae family plants. This paper reviews the results of genetic transformation of Compositae plants, including edible (Cichorium intybus, Lactuca sativa, oil (Helianthus annuus, decorative (Gerbera hybrida, medical (Bidens pilosa, Artemisia annua, Artemisia vulgaris, Calendula officinalis, Withania somnifera etc. plant species. Some Compositae genetic engineering areas are considered including creation of plants, resistant to pests, diseases and herbicides, to the effect of abiotic stress factors as well as plants with altered phenotype. The article also presents the data on the development of biotechnology for Compositae plants Cynara cardunculus, Arnica montana, Cichorium intybus, Artemisia annua "hairy" roots construction.

  8. Use of crop water stress index for monitoring water stress in some sinanthropic plant species

    Directory of Open Access Journals (Sweden)

    Marinela Roxana ROŞESCU

    2010-11-01

    Full Text Available The water stress indicator (crop water stress index, CWSI is a measure of the transpiration rate of a plant, influenced by the leaf and air temperature difference from the plant’s vicinity and the air pressure deficit of the water vapors from the atmosphere. The experiments were realized in July-August 2008 and 2009 for six species in the cities Pitesti, Mioveni and Maracineni: Cichorium intybus L., Conyza canadensis (L. Cronq., Erigeron annuus L. (Pers., Lactuca serriola Torn., Polygonum aviculare L. and Echinochloa crus-galli (L. Beauv. For those species we calculated the CWSI to estimate the water stress on the selected plants in the urban environment conditions. The analyzed species were exposed to a less accentuated water stress while vegetating in the soil and to a more intense one they were grown in the asphalt cracks. Cichorium intybus had the smallest CWSI value (0.26 while Lactuca serriola the highest one (0.44.

  9. Antioxidant Potential of Indigenous Medicinal Plants of District Gujrat Pakistan

    Directory of Open Access Journals (Sweden)

    Mohammad Rafiq Khan

    2014-08-01

    Full Text Available The work reported in this article was carried out to explore hidden antioxidant potential of some medicinal plants of District Gujrat, Pakistan. Crude methanolic extracts of Cichorium intybus L, Malva sylvestris L, and Euphorbia milii L were initially screened by DPPH on TLC assay for their antioxidant activity. Diphenylpicrylhydrayl (DPPH free radical scavenging activity was also determined for all plants. To assess the role of plants in lipid oxidation, PV of refined bleached and deodorized (RBD sunflower oil (SFO at 80°C was monitored. BHT was used as standard antioxidant for comparison. Total phenolic contents (TPC were also calculated. Cichorium intybus L was identified as the richest source of safe natural antioxidants.

  10. Genome size estimates for six rust (Pucciniales) species

    OpenAIRE

    Talhinhas, Pedro; Ramos, Ana Paula; Tavares, Daniela; Tavares, Sílvia; Loureiro, João

    2015-01-01

    Rust fungi (Basidiomycota, Pucciniales), one of the largest groups of phytopathogenic organisms, are characterised by host specialisation, biotrophy, complex life cycles and enlarged genomes. In this work we have used flow cytometry to determine the genome size of six rust species, Melampsora pulcherrima, Puccinia behenis, P. cichorii, P. pimpinellae, P. vincae and Uromyces dianthi, the causal agents of rust on Mercurialis annua, Silene latifolia, Cichorium intybus, Pimpinella villos...

  11. Antioxidant Potential of Indigenous Medicinal Plants of District Gujrat Pakistan

    OpenAIRE

    Mohammad Rafiq Khan; Syed Ali Raza; Mohammad Arshad; Ayoub Rashid Ch; Abdul Razzaq

    2014-01-01

    The work reported in this article was carried out to explore hidden antioxidant potential of some medicinal plants of District Gujrat, Pakistan. Crude methanolic extracts of Cichorium intybus L, Malva sylvestris L, and Euphorbia milii L were initially screened by DPPH on TLC assay for their antioxidant activity. Diphenylpicrylhydrayl (DPPH) free radical scavenging activity was also determined for all plants. To assess the role of plants in lipid oxidation, PV of refined bleached and deodorize...

  12. Vermicomposted olive oil wastewaters in horticultural practices

    OpenAIRE

    Macci, Cristina; Doni, Serena; Peruzzi, Eleonora; Fantoni, Elena; Ceccanti, Brunello; Masciandaro, Grazia

    2012-01-01

    The aim of this study was to evaluate the effect of a vermicompost (obtained from olive mill wastewaters and cellulose materials) on soil quality and fertility, and on the yield of different horticultural species (Allium porrum L.; Cichorium intybus L., Brassica oleracea). The results showed an improvement in the chemical (increase in total and soluble nutrients) and biological (increase in enzyme activities) soil fertility also with respect to a soil treated with traditional municipal solid ...

  13. Use of crop water stress index for monitoring water stress in some sinanthropic plant species

    OpenAIRE

    Marinela Roxana ROŞESCU; Emil CHIŢU

    2010-01-01

    The water stress indicator (crop water stress index, CWSI) is a measure of the transpiration rate of a plant, influenced by the leaf and air temperature difference from the plant’s vicinity and the air pressure deficit of the water vapors from the atmosphere. The experiments were realized in July-August 2008 and 2009 for six species in the cities Pitesti, Mioveni and Maracineni: Cichorium intybus L., Conyza canadensis (L.) Cronq., Erigeron annuus L. (Pers.), Lactuca serriola Torn., Polygonum ...

  14. Effects of European Chicory on Breeding Pregnancy Rate and Litter Performance in Landrace-Yorkshire Crossbred Sows%欧洲菊苣对长大二元母猪配种受胎率及产仔性能的影响

    Institute of Scientific and Technical Information of China (English)

    董殿元

    2014-01-01

    The experiment was conducted to determine the effects of european chicory on breeding pregnancy rate and litter performance in Landrace-Yorkshire crossbred sows.A total 126 2~4 parity's health Landrace-Yorkshire crossbred sows ,which gradually executed the operation of weaning and transferring to non-pregnancy piggery,were randomly allotted to the control group or the european chicory group in the day of weaning.Each group included 63 sows, one sow per replicate.Sow in the control group was fed with lactation diets before estrus,not restricted feed-intake, artificial inseminated after observed estrus, simultaneously transferred into position bar and changed to be fed with early-pregnancy diets (2.0 kg/d), sow in the european chicory group was fed with not only lactation diets but also eu ropean chicory's fresh stem and leaf , not restricted feed-intake , artificial inseminated after observed estrus , simultaneously transferred into position bar and changed to be fed with early-pregnancy diets (1.8 kg/d) and euro-pean chicory's fresh stem and leaf(2.0 kg/d).To observe,discover and record estrus sows within 25 days after insemi-nation.To carry out pregnancy diagnosis by the ultrasonic diagnostic apparatus at the 26th day after insemination, meanwhile pregnancy-determined sow in control group changed to be fed with mid-pregnancy diets (2.5 kg/d),preg-nancy-determined sow in european chicory group changed to be fed with mid-pregnancy diets(2.25 kg/d) and euro-pean chicory's fresh stem and leaf (2.5 kg/d). At the 85th day after insemination,pregnancy-determined sow in control group changed to late-pregnancy diets(3 kg/d),pregnancy-determined sow in european chicory group changed to late-pregnancy diets (2.7 kg/d) and european chicory's fresh stem and leaf (3.0 kg/d).At 3th day before expected date of parturition every sow was transferred into farrowing piggery,observed and litterred in farrowing bed, only fed with lactation diets (3.0 kg/d),ceasing to supply european

  15. 农杆菌介导含硫氨基酸γ-zein 转化菊苣的初步研究%Preliminary studies on transgenic chicory using the sulphur-amino acid gene,γ-zein, mediated by Agrobacterium tumefacien

    Institute of Scientific and Technical Information of China (English)

    张玉; 白史且; 李聪

    2015-01-01

    含硫氨基酸具有动物营养与免疫相关的重要生理功能,为提高菊苣中含硫氨基酸含量,采用根癌农杆菌介导法将玉米种子贮藏蛋白含硫氨基酸基因γ-zein 和绿色荧光蛋白 GFP 融合基因转入到菊苣无菌苗叶片中,经过共培养、潮霉素抗性筛选、分化、再生和炼苗,得到抗性植株。对抗性植株进行 PCR、PCR-Southern、斑点杂交和 RT-PCR 分析,结果表明,外源目的基因已经整合到菊苣基因组中并且得到了表达,为提高菊苣含硫氨基酸含量,改善其品质奠定了基础。%Sulfur-containing amino acids have important physiological functions related to animal nutrition and immunity.To improve the sulfur-amino acid content of chicory,leaves of chicory were transformed with the Sulphur-amino acid gene γ-zein,an important prolamin storage protein from Zea mays and a green fluorescent protein (GFP)gene using Agrobacterium mediated transfusion.After co-culture,selective differentiation and regeneration,hygromycin resistant plants were obtained.Resistant plants were detected using PCR,PCR-southern,dot blot hybridization and RT-PCR.The results demonstrated that the γ-zein genes had been inte-grated into the genome of chicory and expressed on a nucleic acid level in the transgenic plants.

  16. Effect of Chicory Extracts on Gut Flora of Hypertriglyceride in Hyperuricemia Rat Models%菊苣提取物对高甘油三酯并高尿酸血症模型大鼠肠道菌群的影响

    Institute of Scientific and Technical Information of China (English)

    王红坡; 张冰; 林志健; 孙博喻; 牛红娟; 朱春胜; 王雪洁

    2015-01-01

    目的:观察菊苣提取物对高果糖饮水诱导的高甘油三酯并高尿酸血症大鼠尿酸、甘油三酯、总胆固醇水平的影响,并从肠道菌群新的视角探讨菊苣提取物干预高甘油三酯并高尿酸血症模型的特点。方法:雄性SD大鼠按体重随机分为6组,即正常组,模型组,菊苣高剂量组,菊苣低剂量组,非诺贝特组,苯溴马隆组。正常组给予自来水,其余各组均给予10%果糖水,生化法检测血清尿酸、甘油三酯、总胆固醇指标,ERIC-PCR指纹图谱分析各组肠道菌群宏观结构变化。结果:与正常组相比,造模第7天,模型组血清TG水平显著升高;造模第14天,模型组血清TG水平显著升高,血清UA水平有升高趋势;造模第21天,大鼠血清TG、UA均显著升高,大鼠高甘油三酯并高尿酸血症模型塑造成功。与模型组相比,实验第14天,菊苣高剂量,菊苣低剂量,非诺贝特,苯溴马隆均显著降低血清TG水平;实验第21天,菊苣高剂量组显著降低血清TG、UA水平;菊苣低剂量组有降低血清TG和UA水平的趋势;非诺贝特组显著降低血清TG水平;苯溴马隆组显著降低血清UA水平。 ERIC-PCR指纹图谱显示:与正常组相比,模型组肠道菌群结构发生了显著变化,而菊苣高、低剂量组对肠道菌群有干预作用。结论:菊苣提取物治疗高甘油三酯并高尿酸血症作用显著,并可改善紊乱的肠道菌群,说明菊苣提取物可能通过调节肠道菌群发挥治疗作用,具体机制有待于进一步深入研究。%Objective:To investigate the effect of chicory extracts on serum UA,TG and TC levels of hypertrig-lyceride in hyperuricemia rats induced by high fructose water,to discuss the possible characteristics of chicory extracts from the respect of gut flora.Methods: Male SD rats were randomly divided into 6 groups,a normal group,a control group, chicory groups

  17. Effects of Different Chicory and Formula Feed Ratios in Dietary on Slaughter Traits and Muscle Quality of Geese%日粮中菊苣添加比例对鹅屠宰性能和肉品质的影响研究

    Institute of Scientific and Technical Information of China (English)

    张家宏; 韩光明; 马谈斌; 毕建花; 寇祥明; 王守红; 金银根; 唐鹤军; 盖玉芳

    2013-01-01

    研究日粮中菊苣的添加量对扬州鹅屠宰性能、肉品质、氨基酸和脂肪酸组成的影响,确定鹅对菊苣的最大耐受度.结果显示:在菊苣与饲料的比例为12:1组中鹅的全净膛率显著低于对照组(P<0.05),但是腿肌率显著高于对照组(P<0.05),各组中屠体重、屠宰率、半净膛率、胸肌率、翅膀率、腹脂率均无显著差异;日粮中菊苣的比例增加,显著提高了鹅胸肌和腿肌的水分含量和肉色(P<0.05),显著提高胸肌的脂肪含量(P<0.05),显著降低胸肌中蛋白质含量(P<0.05);菊苣添加过量有致使胸肌和腿肌中氨基酸含量下降的趋势,尤其是组氨酸含量在12∶1组中都显著低于其它组(P<0.05);日粮中菊苣添加比例增加,显著提高胸肌中多不饱和脂肪酸含量(P<0.05),腿肌中多不饱和脂肪酸组成差异不显著,无论胸肌还是腿肌,亚麻酸的含量都得到了显著的提高(P<0.05),其中在胸肌中的提高幅度较大.结果说明,菊苣过量添加对扬州鹅的屠宰性能影响较小,但是会影响鹅肉品质、脂肪酸和氨基酸组成.日粮中菊苣添加量越多,鹅肉中的亚麻酸含量越高.%The aim of this study is to examine the effects of different chicory and formula feed ratios in the dietary on the slaughter traits, muscle quality, aniino acid, and fatty acid composition of Yangzhou geese, and determine the maximum tolerance to the added chicory. The results were as follows; in 12: 1 (the ratio of chicory to formula feed in the dietary) group, the goose eviscerated rate was significantly lower than that in the control group (P <0.05) , but the leg muscle rate was significantly higher than that in the control group (P<0.05). The carcass weight, slaughter rate, eviscerated yield with giblet, brisket rate, wing rate and abdominal fat rate in each group had no significant difference. Along with the increase of chicory proportion in the diet, the moisture content and muscle

  18. Produção de mudas de almeirão e cultivo no campo, em sistema agroecológico Agroecological seedling production and field cultivation of chicory

    Directory of Open Access Journals (Sweden)

    Dercio C. Pereira

    2012-10-01

    Full Text Available Objetivou-se, neste trabalho, avaliar o desenvolvimento das mudas de almeirão em diferentes substratos orgânicos e verificar o comportamento em bandejas, após o transplante no campo e na pós-colheita, constituindo três experimentos. Foram avaliados o uso do substrato comercial e quatro misturas de proporções de composto, areia e pó de basalto. Foram quatro fases de avaliação: aos 13 e 26 dias após a emergência (DAE, aos 74 dias após o transplante e na pós-colheita. Avaliaram-se o comprimento e a massa da matéria seca de raiz e de parte aérea, número de folhas por planta, diâmetro do coleto, área foliar e a conservação pós-colheita. O composto orgânico e as misturas foram superiores ao substrato comercial, na maioria das características avaliadas nos 13 e 26 DAE; entretanto, o substrato comercial superou os demais tratamentos para o comprimento de raiz. Os substratos orgânicos (T2 e T3 podem ser recomendados para a produção de mudas de almeirão com desenvolvimento satisfatório em sistema de cultivo em bandejas e a campo e, de igual forma, em pós-colheita; o uso de composto orgânico como substrato para produção de mudas propiciou o desenvolvimento de mudas mais vigorosas e plantas mais resistentes no campo do que o substrato comercial.This study aimed to evaluate the development of chicory seedlings in different organic substrates and verify its behavior in trays, after transplanting in field and in post-harvest, constituting three experiments. Commercial substrate and four mixtures with different proportions of compost, basalt powder and sand were evaluated. Four stages of assessment were: 13 and 26 days after emergence (DAE, at 74 days post-transplant and post-harvest. The length and dry mass of roots and shoots, number of leaves plant-1, stem diameter, leaf area and post-harvest conservation were evaluated. The organic compost and the mixtures were superior to commercial substrate in most of the characteristics

  19. ВИЗНАЧЕННЯ ТЕМПЕРАТУРНОГО РЕЖИМУ ДЛЯ ФОРМУВАННЯ IN VITRO АКТИВНОЇ КОЛЕКЦІЇ БУРЯКУ (Beta vulgaris L.) ТА ЦИКОРІЮ КОРЕНЕПЛІДНОГО (Cichorium intybus L.)

    OpenAIRE

    Рябовол, Л. О.

    2010-01-01

    Визначено умови створення активної колекції генетичного матеріалу рослин буряку цукрового та цикорію коренеплідного за використання методу температурного обмеження в культурі in vitro. Доведено, що температурний режим культурального приміщення на рівні 10°С забезпечує безпересадкове зберігання рослин буряку цукрового протягом десяти місяців, а цикорію коренеплідного — восьми....

  20. Effects of Inulin and Chicory on Glucose Control and Lipid Profiles in Patients with Type 2 Diabetes%菊粉和金玉兰对2型糖尿病患者血糖控制和血脂代谢的影响

    Institute of Scientific and Technical Information of China (English)

    刘鹏举; 马方; 李明; 卢洁

    2015-01-01

    目的:研究菊粉和金玉兰对2型糖尿病患者血糖控制和血脂代谢的影响。方法75例2型糖尿病患者按照性别和体重指数匹配的方法分为菊粉组(n=25)、金玉兰组(n=25)和对照组(n=25),3组患者均给予普通糖尿病膳食,在此基础上菊粉组每天给予菊粉15 g,金玉兰组每天给予金玉兰120 g。分别于基线期、4和8周时观察3组患者的空腹血糖(fasting blood glucose, FBG)、胰岛素抵抗指数(homeostasis model assessment of insulin resistance index, HOMA-IR)、糖化血红蛋白(glycated hemoglobin, HbA1c)、血脂、转氨酶和肌酐等指标的变化并进行比较。结果菊粉组、金玉兰组及对照组分别有23、22和23例完成研究。基线期各项观察指标3组之间差异均无统计学意义( P>0.05)。4周时,菊粉组FBG、 HbA1c、 HOMA-IR、总胆固醇(total cholesterol, TC)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol, LDL-c)的变化同对照组相比有降低的趋势;8周时,菊粉组FBG、 HbA1c、 HOMA-IR、 TC、 LDL-c的降低同对照组相比差异具有统计学意义(P<0.05),其余观察指标变化的差异无统计学意义(P>0.05)。4周和8周时,金玉兰组各项指标变化同对照组相比差异无统计学意义( P>0.05)。结论菊粉有益于2型糖尿病患者的血糖控制和血脂代谢,并且对肝、肾功能无影响。金玉兰对2型糖尿病患者血糖控制和血脂代谢无显著影响。%Objective To investigate the effects of inulin and chicory on glucose control and lipid profiles in patients with type 2 diabetes.Methods Seventy-five patients with type 2 diabetes were assigned to inulin group (n=25), chicory group (n=25), and control group (n=25) with matching gender and body mass in-dex.The three groups were all given diabetes diets, in addition, 15 g inulin and 120 g chicory daily were added in the inulin group

  1. 菊苣多糖对糖尿病大鼠心肌缺血再灌注过氧化的影响%The protective effect of chicory polysaccharides on diabetic-resulted myocardial ischemia-reperfusion injury in rats

    Institute of Scientific and Technical Information of China (English)

    李屹; 刘晓舒; 杨鑫; 马越娇; 张建军; 车光昇; 宋光熠

    2013-01-01

    Objective To observe the protective effect of chicory polysaccharides on myocardial ischemia-reperfusion injury resulted by streptozotocin diabetic in rats. Methods Streptozotocin intraperitoneal was used to induce diabete( DM) rat model. After 12-week injection, DM rats were anesthetized with pentobarbital, TI and breathing machine installed. Then DM rats were ligated descending branch of CA on LV for 60 minutes, and loosed the ligation to realize the reperfusion for 60 min. Heart and blood were collected for testing CK, LDH, MDA, SOD, and GSH-PX. Results CK, LDH and MDA in Chicory Polysaccharides group were significantly lower than those of DM group, however, the activities of SOD and GSH-Px turned out just opposite. Conclusions Chicory polysaccharide definitely has a protective effect to the diabetic-resulting myocardial ischemia-reperfusion injury in rat.%目的 探讨菊苣多糖对链脲菌素糖尿病大鼠心肌缺血再灌注损伤过氧化的影响.方法 应用链脲菌素复制大鼠糖尿病模型,12w后,腹腔注射戊巴比妥钠麻醉下气管插管安装呼吸机,开胸结扎冠状动脉左室降支60 min后松开结扎线再灌注60 min.标Ⅱ导联全程记录心电图,并于再灌注60 min取血、心脏.检测肌酸激酶(CK)、乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px).结果 菊苣多糖组的CK、LDH、MDA水平明显低于糖尿病组,SOD、GSH-PX活性高于糖尿病组(均P<0.05).结论 菊苣多糖通过抗过氧化作用对链脲菌素糖尿病大鼠心肌缺血再灌注损伤具有保护作用.

  2. Crescimento e acúmulo de macronutrientes em chicória coberta e não coberta com polipropileno Growth and accumulation of macronutrients on chicory, covered or not with polypropylene

    Directory of Open Access Journals (Sweden)

    Anderson Luiz Feltrim

    2008-03-01

    Full Text Available O experimento foi realizado com o objetivo de comparar o crescimento e acúmulo de macronutrientes pela chicória coberta e não coberta com tecido de polipropileno (TP, branco de 20 g m-2. O delineamento experimental adotado foi de blocos ao acaso em parcelas subdivididas, com 4 repetições. Na parcela, os quatro tratamentos constituíram-se de duas cultivares de chicória crespa (Chicória Crespa e AF-218 em dois sistemas de cultivo (com e sem tecido de polipropileno e nas subparcelas as épocas de avaliação da chicória, correspondentes às idades da planta (7; 14; 21; 28; 35 e 42 dias após o transplante. A semeadura foi realizada em 02/06/03 e o transplante em 27/06/03. Foi avaliada semanalmente a altura da parte aérea, número de folhas, diâmetro das plantas, matéria fresca e seca da parte aérea e o acúmulo de macronutrientes. Exceto para o diâmetro da parte aérea, observou-se superioridade das demais características nas plantas que receberam a cobertura com TP. A cobertura com TP por todo o período pós-transplante proporcionou maior acúmulo de P, K, Mg e S. Plantas da 'AF-218' cobertas com TP por todo período pós-transplante, e que apresentaram maior matéria fresca da parte aérea, acumularam 836; 515; 205; 144; 90 e 65 mg planta-1 de N, K, Ca, Mg, S e P, respectivamente.The experiment was carried out to compare the growth and accumulation of macronutrients on chicory, covered or not with white polypropylene (TP, 20 g m-2 thickness. The experimental design was of randomized blocks with divided-plots in four repetitions. In the plots, the four treatments consisted of two curly endive cultivars (Crespa and AF-218 in two cultivation systems (with and without polypropylene and the subplots consisted of the plant age (7; 14; 21; 28; 35 and 42 days after transplant. Seeds were sown on June 2nd, 2003, and transplanted on June 27th. The aerial plant part, number of leaves, diameter of plant, fresh and dry matter of the aerial

  3. 菊苣航天诱变新品系叶片旱生结构的比较研究%Study on Anatomical Structures Related to Drought Resistance of Leaves of New Strains of Chicory Mutated in Space

    Institute of Scientific and Technical Information of China (English)

    韩永芬; 卢欣石; 孟军江; 王铁梅; 舒健虹

    2011-01-01

    对15个菊苣空间诱变新品系叶片进行解剖,选取9项抗旱指标,采用单因素分析方法对各品系进行两两多重比较,得到各指标的灵敏度,以下表皮气孔密度和气孔长度、维管束厚度、叶片厚度4个指标为主进行聚类分析.结果表明不同菊苣新品系叶片的抗旱性解剖结构除表皮厚外差异均达极显著水平.各指标的灵敏度大小依次为:下表皮气孔密度>下表皮气孔长度>上表皮气孔长度>上表皮气孔密度>维管束>叶片厚度、叶脉厚度>下、上表皮厚度.聚类分析结果表明,15个菊苣新品系可聚为3个级别:其中PA-95、PA-96、PA-11、PA-93、PA-49、PA-20、PA-82、PA-42具有较好的抗旱性结构;PA-36、PA-31、PA-57、PA-186、PA-54、PA-26抗旱性结构中等;PA-43的抗旱性结构较差.%The anatomical structures related to drought resistance of the leaves of 15 new chicory strains by space mutagenesis were studied based on 9 xeromorphic indexes, the single factor multiple comparison was done between two strains in order to conclude the sensitiveness sequence of the 9 indexes to the drought ,and the clustering analysis was conducted based on four most sensitive indexes including the stoma density on lower epidermis, the stoma length,the thickness of bundle,the thickness of leaves. The results showed that there was highly significant difference between the strains. The sequence of the sensitivity was as follows: lower epidermis stoma density >lower epidermis stoma length > upper epidermis stoma length >upper epidermis stoma density >bundle thickness > leaf thickness, midrib thickness>upper and lower epidermis thickness. And according to clustering analysis,the 15 strains could be divided into three classes in terms of the ability of the leaves structures against the drought:PA-95, PA-96 ,PA -ll,PA-93,PA-49,PA-20 ,PA-82,PA-42 were the best, while PA-43 were the worst, and PA -36, PA-31, PA-57, PA-186, PA-54 and

  4. First Report of the Carolina Spiral Nematode, Scutellonema brachyurus, from Soil of a Garden in Crete, Greece.

    Science.gov (United States)

    Tzortzakakis, Emmanuel A; Cantalapiedra-Navarrete, Carolina; Archidona-Yuste, Antonio; Palomares-Rius, Juan E; Castillo, Pablo

    2016-03-01

    Members of the genus Scutellonema can cause substantial crop losses to ornamental and cultivated plants directly by feeding ectoparasitically on plant roots (Bridge et al., 2005; Coyne et al., 2006). In May 2015, a soil sample from a house garden from Heraklion city in Crete, Greece, was sent for diagnosis of plant-parasitic nematodes. In this place, there had been cactus (Opuntia sp.) plants (probably imported), which were uprooted 3 to 4 years earlier. After that, the area was cropped with cucumber (Cucumis sativus L.) in spring-summer and leaf vegetables such as spinach (Spinacia oleracea L.) and chicory (Cichorium intybus L.) in autumn-winter. The soil was collected 1 mon after the end of chicory crop. A population density (ca. 30 individuals/100 cm(3) of soil) of spiral nematodes (Scutellonema sp.) was found by extracting soil with the wet sieving and decanting method (Cobb, 1918). Morphological and molecular analyses of females identified the species as Scutellonema brachyurus (Steiner, 1938) Andrássy, 1958. The morphology of females was characterized by a hemispherical lip region with four to six annuli, morphometric data for 12 females were L, 640 to 760 μm; a, 24.6 to 30.6; b, 5.8 to 7.4; c, 69.1 to 99.3; c´ 0.5 to 0.6; stylet, 24.5 to 27.5 μm with anterior part shorter than posterior; and spermatheca nonfunctional and male absent. The morphology agreed with the description of S. brachyurus (van den Berg et al., 2013). Alignment indicated that the D2-D3 and ITS sequences (KU059494 and KU059495, respectively) showed 99% and 100% to 99% similarity, respectively, to other sequences of S. brachyurus (type A) deposited in GenBank from the United States, Italy, and Korea (JX472037-JX472046, DQ328753, FJ485643; and JX472069, JX472070, JX472071, respectively), differing from one to six nucleotides. Phylogenetic analyses using Bayesian inference of these sequences placed the Scutellonema sp. in a highly supported (100%) clade that included all S. brachyurus

  5. Caracterização reológica das diferentes fases de extrato de inulina de raízes de chicória, obtidas por abaixamento de temperatura Rheological characterization for different phases of inulin extract from chicory roots, obtained through temperature reduction

    Directory of Open Access Journals (Sweden)

    Juliana T. C. Leite

    2004-04-01

    Full Text Available A inulina é um ingrediente funcional, geralmente empregado na indústria alimentícia como substituto do açúcar ou da gordura. Esse ingrediente pode ser encontrado em diversos produtos vegetais, incluindo as raízes de chicória. Por ser um produto com solubilidade variável com a temperatura, a inulina sofre uma separação de fases ao ser resfriada, originando uma fase precipitada, mais viscosa, e uma fase sobrenadante, de menor viscosidade. O estudo das propriedades reológicas das diferentes fases do extrato de inulina é importante para o projeto de equipamentos, como misturadores e bombas. Neste trabalho, foi estudado o comportamento reológico, para três condições distintas de temperatura (25; 40 e 50 ºC, das fases sobrenadante e precipitada do extrato líquido de inulina, extraído de raízes de chicória por difusão em água quente e resfriado a duas temperaturas distintas (8 e -10 ºC, sofrendo separação de fases. A fase precipitada foi analisada em duas condições: pura e com a adição de agentes microencapsulantes. Todos apresentaram um comportamento linear, semelhante ao dos "Plásticos de Bingham", porém, nem todos se ajustaram a esse modelo.Inulin is a functional food ingredient, generally employed as sugar or fat substitute in food systems. This ingredient can be found in several vegetal products, including chicory roots. As the solubility of inulin is susceptible to temperature changes, the product suffers a fractionalization resulting in two phases when cooled, originating a precipitated phase, more viscose, and a liquid phase, of lesser viscosity. The study of rheological properties of different phases of inulin extract is important for equipment designing, such as mixer and bombs. In this work, rheological behavior at three different temperatures (25; 40 and 50 ºC was determined for liquid and precipitated phases of inulin liquid extract, extracted from chicory roots by hot water diffusion and cooled at two

  6. Produção de almeirão em função de níveis de fertirrigação nitrogenada e disposição de mangueiras gotejadoras nos canteiros Chicory yield under levels of nitrogen fertirrigation and position of drip hoses on the ridge

    Directory of Open Access Journals (Sweden)

    Guilherme Augusto Biscaro

    2012-10-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos de diferentes níveis de fertirrigação nitrogenada na produtividade e nas características agronômicas de plantas de almeirão, irrigadas por gotejamento. O delineamento experimental foi o de blocos casualizados em esquema de parcelas subdivididas, sendo avaliadas nas parcelas duas formas de disposição das mangueiras gotejadoras (superficialmente sobre o solo do canteiro e subsuperficialmente e nas subparcelas seis doses de nitrogênio: 0, 30, 60, 90, 120 e 150kg ha-1. A forma de disposição das mangueiras afetou a produção apenas da massa fresca, com o melhor resultado sendo obtido com a irrigação subsuperficial. A produção e as características agronômicas do almeirão foram influenciadas pelas doses de nitrogênio aplicadas na água de irrigação nas duas formas de disposição das mangueiras nos canteiros. A maior eficiência financeira da adubação foi obtida com a dose de 30kg ha-1 de N nas duas formas de disposição das mangueiras gotejadoras.In this research the objective was to evaluate the effects of different levels nitrogen fertirrigation on yield and agronomic characteristics of chicory plants irrigated by drip.The experimental design was a randomized block in split plot with two ways of using drip hoses on the ridge (on the surface and subsurface of soil and six levels of nitrogen, 0, 30, 60, 90, 120 and 150kg ha-1.The hose layout affected the yield fresh weight only with better results obtained with subsurface irrigation.The yield and agronomic characteristics of chicory were influenced by nitrogen levels applied in irrigation water in the two forms of hoses disposition in the beds. The best economic efficiency of fertilization was obtained with 30kg N ha-1 in both forms of drip hoses disposition.

  7. Toxicity of some plant extracts against vector of lymphatic filariasis, Culex pipiens.

    Science.gov (United States)

    Hasaballah, Ahmed I

    2015-04-01

    Many insecticides are generally used as larvicides to control Culex pipiens, vector of lymphatic filariasis. This study was undertaken to evaluate the larvicidal activity of some potential larvicidal plants extracts against C. pipiens larvae. The toxic effects of both ethanolic and petroleum ether plant extracts were evaluated under laboratory conditions against 3rd instar larvae of C. pipiens. Forty ethanolic and petroleum ether extracts of 10 plants namely Echinochloa stagninum, Phragmites australis, Eichhornia crassipes, Rhizophora mucronata, Cichorium intybus, Ocimum basilicum, Origanum majorana, Azadirachta indica, Rosmarinus officinalis and Nigella sativa. On the basis of LC50, the toxic effect of the plant extracts tested varied depending on the plant species, part, solvent used in extraction and the extract concentrations. The petroleum ether extraction was more effective against mosquito as compared with ethanolic extraction. The most effective plant extract was A. indica followed by Ph. australis, N. sativa, C. intybus, R. officinalis, O. basilicum, O. majorana, E. stagninum, Rh. Mucronata and E. crassipes. PMID:26012233

  8. 菊苣对高尿酸血症鹌鹑肾脏有机阴离子转运体OAT3-LIKE的影响%Effect of Chinese Herb Chicory on Renal Organic Anion Transporter OAT3-LIKE of Hyperuricemia Quails

    Institute of Scientific and Technical Information of China (English)

    李丽玉; 林志健; 张冰; 王雪洁; 朱春胜; 牛红娟; 周月; 董百惠

    2015-01-01

    目的:从肾脏转运蛋白角度探究中药菊苣降尿酸的药效机制。方法将40只雄性迪法克鹌鹑按体质量随机分为5组,正常组给予普通饲料,其余各组均给予含酵母饲料(15 g·kg-1·d-1)建立高尿酸血症模型,同时给药组分别给予菊苣5,7.5 g·kg-1·d-1,苯溴马隆20 mg·kg-1·d-1,实验第35天处死。检测鹌鹑血清尿酸(Uric Acid,UA)、尿素氮(Urea Nitrogen,BUN)水平,并用Real-time RT-PCR(Real-time Reverse Transcription-Polymerase Chain Reaction)方法检测其肾脏有机阴离子转运体OAT3-LIKE(Organic Anion Transporter3-LIKE)的mRNA表达水平,同时测定肝脏尿酸代谢相关酶活性。结果与正常组比较,实验第21天、28天、35天模型组鹌鹑血尿酸升高,第35天肾脏OAT3-LIKE mRNA表达水平下降。与模型组比较,实验第35天,菊苣可以降低鹌鹑血尿酸水平,上调其肾脏OAT3-LIKE mRNA表达水平并降低肝脏腺苷脱氨酶(ADA)活性。结论高嘌呤食饵可以成功诱导鹌鹑高尿酸血症;菊苣具有抑制高尿酸血症鹌鹑尿酸生成和促进尿酸排泄的双重作用;菊苣药效机制可能与降低ADA活性及上调OAT3-LIKE表达水平相关。%Objective To explore the pharmacodynamic mechanism of chicory on lowering uric acid from the aspects of renal transport protein. Methods Forty male quails were randomly divided into five groups. The normal group was fed with common forage, and the other groups were fed with forage containing yeast(15 g·kg-1·d-1) to establish hyperuricemia model. At the same time,the medication groups were given chicory in the dosage of 5,7.5 g·kg-1·d-1, and benzbromarone 20 mg·kg-1·d-1, respectively. The quails were sacrificed on the 35th day. We detected serum uric acid(UA) and urea nitrogen levels and hepatic adenosine deaminase(ADA) activity, and examined the expression levels of renal organic anion transporter protein OAT3-LIKE mRNA using real

  9. Appraisal of different plant products against trogoderma granarium everts to protect stored wheat - a laboratory comparison

    International Nuclear Information System (INIS)

    Laboratory study was initiated to analyze various locally available botanical materials such as Chenopodium album L. (White goose foot), Cichorum intybus L. (Chicory) and Meliolotus parviflora L. (Honey clover) applied at the rate of 2 percent into wheat grain for the control of Trogoderma granarium Everts (Coleoptera: Dermestidae). After data computation on the basis of fixed parameters, it was observed that all natural protectants used had shown potentiality in controlling T. granarium as these proved very effective compared to no pesticide application treatment. It is worthwhile to mention that among various products tested, C. intybus powder showed the best results to control pest, approached by C. album and M. parviflora treatments as compared to untreated kernels. The results of this findings show that natural protectants used in the study could be useful and desirable tools in stored wheat pest management plan. (author)

  10. [The in vitro action of plants on Vibrio cholerae].

    Science.gov (United States)

    Guevara, J M; Chumpitaz, J; Valencia, E

    1994-01-01

    Natural products of several plants, according to the geographic location, are used by Peruvian people in the popular treatment of diarrhea, with good success. When cholerae cases appeared in Peru, we were interested to know the "in vitro" effect against Vibrio cholerae 01, of these useful plants to treat diarrhea. The following plants were tested: Cichorium intybus, Althaea officinalis, Psorela glandulosa, Geranium maculatum, Punica granatum, Malus sativa, Cydonia oblonga, Chenopodium ambrosoides, Krameria triandria, Tea chinensis, Daucus carota, Persea gratissima, Psidium guayaba and Lippia dulcis. Decoction or infusion of the plants were used in the "in vitro" experiments. The following plants showed no "in vitro" effect against V. cholerae: Cichorium intybus, Althaea officinalis, Psorela glandulosa, Geranium maculatum, Chenopodium ambrosoides, Krameria triandria, Psidium guayaba, Lippia dulcis and Daucus carota. Decoction of Malus sativa and Cydenia oblonga showed bactericidal effect for their acidity and stone avocado (Persea gratissima) a late bactericidal effect. Tea infusión and the decoction of Punica granatum peel, showed the best bactericidal effect and we suggest to use them as to stop cholera spreading. PMID:8018898

  11. ИССЛЕДОВАНИЕ МОЛЕКУЛЯРНОЙ СТРУКТУРЫ ЛЕКАРСТВЕННЫХ РАСТЕНИЙ ОДУВАНЧИКА ( TARAXACUM OFFICINALE WIGG.) И ЦИКОРИЯ ОБЫКНОВЕННОГО ( CICHORIUM INTYBUS L.) МЕТОДОМ СПИНОВОЙ МЕТКИ

    OpenAIRE

    Юсупов, И.; Бахдавлатов, А.; Марупов, Р.

    2012-01-01

    Разработана методика химической модификации дикорастущих лекарственных растений одуванчика и цикория методом спиновой метки. Изучена стабильность и молекулярная подвижность нитроксильного радикала, введённого в матрицу одуванчика и цикория. Установлено, что при комнатной температуре в спектрах ЭПР наблюдается заторможенность вращательной подвижности спиновой метки с временем корреляции t c≤10 -7c, которая свидетельствует об изменении системы межи внутримолекулярных водородных связей в области...

  12. Development of base populations from root and tuber crops for the production of sugar and starch as raw material for the industry

    Energy Technology Data Exchange (ETDEWEB)

    Frese, L.; Schittenhelm, S.; Dambroth, M.

    1987-01-01

    Based on results of evaluation and breeding programmes with beets, potato, Jerusalem artichoke and chicory the following conclusions can be drawn: For breeding beets with a high sugar yield it seems favourable to produce hybrids between sugar and fodder beet. Compared to the average of their parents the sugar beet x fodder beet and fodder beet x sugar beet hybrids produced 3,8 and 12,9% resp., higher sugar yields. It is, however, from this experiment not fully clear if heterosis allone is the explanation for this increase. Several preselected populations of the primitive potato species S. tuberosum subsp. andigena and S. phureja from the United States, Scotland and the Netherlands have been tested in a field trial. Compared to Phureja the Andigena material has reached a high level of adaptation. An immediate use in commercial breeding programmes for the production of high yielding Tuberosum-Andigena hybrids is possible. In Jerusalem artichoke the production of new genetic variability by crossing is complicated by the bad fertility of this species. Because of the very limited breeding activities in the past a large advance is possible. Even though the species C. intybus is of many shapes, only the root chicory is suitable for use as an industrial crop. Leaf chicory produces only low to medium sugar yields and is badly adapted to mechanical harvest. (orig.)

  13. ATTEMPT TO REDUCE ACRYLAMIDE CONTENT IN ROASTED CHICORY

    Directory of Open Access Journals (Sweden)

    Gabriela Zięć

    2015-02-01

    Full Text Available The region of Upper Nitra has been influenced for several decades by the intensive activity of the industry. The Nitra is a river with the most polluted water ecosystems. It was caused by anthropogenic activities in the past decades. In the upper reaches of the river in the cadastral territory of the village Zemianske Kostoľany is located the thermal power plant. There is the sedimentation reservoir, which caused the ecological disaster in 1965. During this accident there was a bursting of the dam and the downstream sedimentation of sludge spill, which contaminated the entire water catchment area of the Nitra river. As a result of an ecological accident occurred to a long-term contamination of the stream but also the adjacent alluvial areas by heavy metals (Hg, As, Pb and others. In the present work, we have focused on ascertaining the level of contamination the adjacent plot of land and seeds of corn in the cadastral territory of Malé Kršteňany. We ascertained the excess of the limit values in the case of Hg on the south side of the agricultural land in Malé Kršteňany, which borders with the stream of the river. The Cd content has exceeded the limit value at the five sampling places, in the case of lead, content does not exceed the limit value. The average mercury concentration in the grain of maize was 0.095 mg.kg-1, which exceeds the limit value by 90.5%. The average cadmium content in maize grain reached the value of 0.06 mg.kg-1. This value does not exceed the limit value. Average lead accumulation in the grain of maize was 0.54 mg.kg-1, which exceeds 2.7 times the limit value. The results of analyzes indicate an increased level of land contamination by mercury and cadmium, which was reflected in an increased level of contamination by monitored metals of crops.

  14. APLICACIÓN DE LA TECNICA DE IV GAMA PARA LA ELABORACIÓN DE ENSALADAS EVALUATION OF IV GAMA TECHNIQUE TO PREPARE VEGETABLE SALADS

    Directory of Open Access Journals (Sweden)

    Auris Damely García Méndez

    2008-12-01

    Full Text Available El propósito de la aplicación de la técnica de IV Gama en la elaboración de ensaladas listas para el consumo fresco, esta dirigido al control del deterioro en lechuga americana (Lactuca sativa, zanahoria (Daucus carota L., radicchio (Cichorium intybus L. y escarola (Cichorium endivia L., destinadas a preparar ensaladas tipo “Mediterránea y Cheff”. La metodología permitió seleccionar y evaluar la técnica de IV Gama, así como las buenas prácticas de manufactura para extender la vida comercial de las ensaladas y garantizar la seguridad alimentaria para la población potencialmente consumidora. La aplicación combinada de soluciones de cloruro de calcio al 1%, acido cítrico a 0,1% y oxido de magnesio al 0,5% contribuyo a mantener la calidad de las ensaladas por un período de 16 días bajo refrigeración a 5 + 1 °C y 95 + 5% HR. En conclusión las técnicas ensayadas, posibilitaron extender el valor comercial de estos productos.The purpose of applying the fresh-cut technique in preparing vegetable salads ready for fresh consumption, was aimed to control the damage American lettuce (Lactuca sativa; carrot (Daucus carota L; Radicchio (Cichorium intybus L and endive (Cichorium endivia L.“ Mediterranean and Cheff” style salads were prepared for evaluation. The methodology allowed selecting and evaluating fresh-cut technique as well as good manufacturing practices, to extend the commercial life, and guarantee alimentary food safety to people. Use of 1% calcium chloride; 0.1% citric acid and 0.5% magnesium oxide solutions; allowed to maintain the quality of the salads for a period of 16 up to days, under refrigeration at 5± 1ºC and 95 ± 5 % RH. Finally the assays techniques let to extend the commercial value of these vegetables.

  15. Crescimento e produtividade do almeirão em concentrações de N total contendo NH4+ na solução nutritiva Growth and yield of chicory plants under total N concentrations containing NH4+ in the nutrient solution

    Directory of Open Access Journals (Sweden)

    Rodrigo dos Santos Godoi

    2007-08-01

    Full Text Available O crescimento e a produtividade do almeirão foram determinados em cultivo hidropônico fechado com cinco concentrações de N contendo NH4+ na solução nutritiva. As cultivares "Amarelo" e "Pão de Açúcar" foram semeadas diretamente em fileiras distanciadas de 0,05m, em uma camada de areia de 0,15m, distribuída sobre telhas de fibrocimento. Após a emergência, foi efetuado o desbaste, mantendo-se uma densidade de 1.600plantas m-2. Foi empregada uma solução nutritiva padrão com a composição de, em mmol L-1, 11,0 de NO3-; 1,5 de H2PO4-; 6,5 de SO4-2; 7,5 de Ca+2; 10,0 de K+ e 1,5 de Mg+2 e, em mg L-1, 0,42 de Mn; 0,26 de Zn; 0,05 de Cu; 0,50 de B; 0,04 de Mo, e 4,82 de Fe quelatizado. Os tratamentos foram constituídos por concentrações totais de N de 11,0 (T1; 16,0 (T2; 21,0 (T3; 26,0 (T4 e 31,0 (T5mmol L-1, contendo concentrações de NH4+ de, zero; 2,5; 5,0; 7,5 e 10,0mmol L-1, respectivamente. O delineamento experimental inteiramente casualizado foi empregado, com quatro repetições e parcelas subdivididas. Foram efetuadas colheitas aos 35, 59, 76 e 97 dias após a semeadura, mediante corte da parte aérea das plantas a 0,02m acima da superfície da areia, para determinar a matéria seca e fresca. A maior produtividade foi obtida com a solução nutritiva contendo 16mmol L-1 de N total e 2,5mmol L-1 de NH4+.Chicory plants, cv. Amarela and Pão de Açúcar, were hidroponically grown to determine growth and yield under five ammonium and nitrate N concentrations in the nutrient solution. Sowing was made in rows placed 0.05m apart, over a 0.15m deep sand growing bed supported by fibber cement tiles. After emergency, plants were thinned to a plant density of 1,600plants m-2. A standard nutrient solution was used, with the composition of, in mmol L-1, 11.0 de NO3-; 1.5 H2PO4-; 6.5 SO4-2; 7.5 Ca+2; 10.0 K+ and 1.5 Mg+2, and, in mg L-1, 0.42 Mn; 0.26 Zn; 0.05 Cu; 0.50 B; 0.04 Mo, and 4.82 chelated Fe. Treatments were total N

  16. Eriophyoid mite fauna (Acari: Trombidiformes: Eriophyoidea) of Turkey: new species, new distribution reports and an updated catalogue.

    Science.gov (United States)

    Denizhan, Evsel; Monfreda, Rosita; Lillo, Enrico De; Çobanoğlu, Sultan

    2015-01-01

    More than one hundred species of Eriophyoidea have been recorded hitherto from Turkey. Within the last decade, a large plant survey was carried out in order to investigate the eriophyoid fauna present in Turkey, with particular emphasis on species affecting weeds and ornamental plants. In addition, the Turkish literature has been examined for previous records of eriophyoid mites. New species, Paraphytoptus intybi n. sp. on common cichory, Cichorium intybus (Compositae) and Phytoptus albae n. sp. on white poplar, Populus alba (Salicaceae), are described and illustrated herein. In addition, a further 31 species were found to be new records for the eriophyoid fauna of Turkey with Aceria calaceris, Phyllocoptes didelphis and Vasates immigrans being new reports for the Palaearctic region. All known eriophyoid species records from Turkey appearing in papers published up until March 2013 are listed in this catalogue along with remarks and information on their current distribution in Turkey and taxonomic status. PMID:26250255

  17. Elemental characterization of wild edible plants from countryside and urban areas.

    Science.gov (United States)

    Renna, Massimiliano; Cocozza, Claudio; Gonnella, Maria; Abdelrahman, Hamada; Santamaria, Pietro

    2015-06-15

    Thirteen elements (Na, K, Ca, Mg, Fe, Mn, Cu, Zn, Cr, Co, Cd, Ni and Pb) in 11 different wild edible plants (WEP) (Amaranthus retroflexus, Foeniculum vulgare, Cichorium intybus, Glebionis coronaria, Sonchus spp., Borago officinalis, Diplotaxis tenuifolia, Sinapis arvensis, Papaver rhoeas, Plantago lagopus and Portulaca oleracea) collected from countryside and urban areas of Bari (Italy) were determined. B.officinalis and P.rhoeas could represent good nutritional sources of Mn and Fe, respectively, as well as A.retroflexus and S.arvensis for Ca. High intake of Pb and Cd could come from P.lagopus and A.retroflexus (1.40 and 0.13 mg kg(-1) FW, respectively). WEP may give a substantial contribution to the elements intake for consumers, but in some cases they may supply high level of elements potentially toxic for human health. Anyway, both ANOVA and PCA analyses have highlighted the low influence of the harvesting site on the elements content. PMID:25660854

  18. 普那菊苣替代部分精料饲喂猪的效果试验

    Institute of Scientific and Technical Information of China (English)

    周振桓; 李庆华; 段婕; 胡国华; 徐军

    2008-01-01

    菊苣(cichorium intybus L)是一种多年生菊科草本植物,有多个变种,分布于欧洲、亚州、北非和南美,在国外广泛用作饲料、蔬菜和制糖原料。20世纪80年代,新西兰科学家利用原生长于俄罗斯外高加索地区的一个菊苣株系,成功地选育出了理想的饲用品种,称之为普那菊苣(Puna chicory),

  19. STUDY ON POLLEN VIABILITY AS BIOINDICATOR OF AIR QUALITY

    Directory of Open Access Journals (Sweden)

    Florentina ŞTEFLEA

    2012-01-01

    Full Text Available The aim of this research is to estimate the relationship between pollen viability and atmospheric pollution (in polluted and non-polluted conditions. The study was carried out in the city of Timisoara. Two areas, with different intensity of road traffic (very high and absent but all characterized by the presence of the same plant species, were selected. The pollen of herbaceous spontaneous species, arboreal species and a shrub species was used (Robinia pseudacacia, Aesculus x carnea, Catalpa bignonioides, Albizzia julibrissin, Rosa canina, Sambucus nigra, Malva neglecta, Ranunculus acer, Trifolium repens, Cichorium intybus. The pollen of these species was treated with TTC (2, 3, 5 Tryphenil-Tetrazolium-Chloride staining solution and viability was then estimated by light microscopy. The results of the mean pollen viability percentage of the examined species are reported. Pollen viability of herbaceous plants is significantly different between the two environments.

  20. Phytochemical and Pharmacological Standardisation of Polyherbal Tablets For Hepatoprotective Activity Against Carbon Tetrachloride Induced Hepatotoxicity

    Directory of Open Access Journals (Sweden)

    Vilas A. Arsul

    2010-10-01

    Full Text Available Literature survey revealed that phyllanthin and hypophyllanthin from Phyllanthus niruri, wedenolactone from Eclipta alba, and kutkin from Picrorhiza kurroa are responsible for hepatoprotective activity, and phenolic and flavonoids are responsible for antioxidant activity. A selected polyherbal formulation composed of 7 herbal extract mixtures such as Phyllanthus niruri, Eclipta alba, Cichorium intybus, Boerhaavia diffusa, Embelia ribes, Berberis aristata and Picrorhiza kurroa. The phytochemical evaluation was carried out by estimation of total phenolic content and total flavonoids. The antioxidant activity was compared with ascorbic acid (ASC and Rutin as standard. The hepatoprotective activity in carbon tetrachloride induced hepatotoxicity were studied. Assessment of liver function was made by estimating the activities of SGOT, SGPT, ALP, Cholesterol, Bilirubin and Total protein. From the study it is seen that formulation exhibit significant activity.

  1. Flora in abandoned fields and adjacent crop fields on rendzina soils in the Zamość region

    Directory of Open Access Journals (Sweden)

    Marta Ziemińska-Smyk

    2015-10-01

    Full Text Available A floristic inventory of segetal flora was carried out in abandoned fields and adjacent crop fields on rendzina soils in the Zamość region in the year 2010. This study found a total of 130 weed species belonging to 30 botanical families. The following families were represented most frequently: Asteraceae, Fabaceae, Poaceae, Lamiaceae, Scrophulariaceae, and Brassicaceae. In the segetal flora, apophytes are dominant (55% of the total flora, with the highest number of meadow and xerothermic grassland species among them. Archeophytes (38% predominate in the group of anthropophytes. The species characterized by the highest constancy classes and reaching the highest cover indices posed the greatest threat to crops in the study area. The following weeds are most frequently found in fallow fields: Consolida regalis, Cichorium intybus, and Sinapis arvensis, while Papaver rhoeas is the greatest threat to cereal crops grown on rendzina soils.

  2. Eriophyoid mites (Acari: Prostigmata: Eriophyidae) associated with Compositae in Iran.

    Science.gov (United States)

    Lotfollahi, Parisa; Irani-Nejad, Karim Haddad; Khanjani, Mohamad; Moghadam, Mohamad; De Lillo, Enrico

    2013-01-01

    Five species of eriophyoid mites were identified during surveys of mite fauna associated with plant species of the family Compositae from Southwest of East Azerbaijan province during 2010 and 2011. Two of them, Aceria virgatae n. sp. from Centaurea virgata Lam. and Aceria xeranthenzis n. sp. from Xeranthemumn squarrosum Boiss., were found to be new to science. No damage symptoms were observed on their host plants. Aceria xeranthemis n. sp. is the first eriophyoid collected from the plant genus Xeranthenun. Aculops centaureae (Farkas, 1960) from Centaurea albonitens Turrill and Aceria cichorii Petanović et al. 2000 from Cichorium intybus L. are new records for Iranian mite fauna. The deutogyne female of Aceria anthocoptes (Nalepa) was recorded for the first time in Iran, too. A key to the species collected on Compositae in Iran is given. PMID:26266306

  3. Cadmium and chromium concentrations in six forage species irrigated with canal, sewage or mixed canal and sewage water

    International Nuclear Information System (INIS)

    Cadmium (Cd) and chromium (Cr) concentrations were investigated in six different forage species, i.e., Trifolium alexandrium, Cichorium intybus, Avena sativa, Medicago polymorpha, Brassica campestris and Medicago sativa, irrigated with canal, sewage or mixed canal and sewage water. The Cd levels of the forages irrigated with different water treatments in this study were greater than the tolerance level for grazing livestock reported in the literature. Thus, the Cd levels in the six plant species found in the current study showed high potential threat for livestock consuming these forages. Similarly, high levels of Cr in the six forages were observed with sewage water treatment only. However, in the present investigation, Cr concentration of forages was below the toxic level. Therefore, there was no threat of Cr toxicity for animals being reared on these forages. (author)

  4. Use of herbal remedies among patients undergoing hemodialysis.

    Science.gov (United States)

    Roozbeh, Jamshid; Hashempur, Mohammad Hashem; Heydari, Mojtaba

    2013-11-01

    This study aims to determine the prevalence, types, and associated factors for the use of herbal remedies in hemodialysis patients. Two hundred participants were selected by stratified sampling and were systematically interviewed. One hundred and twenty-six patients (63%) had used herbal remedies some time since their initiation of dialysis treatment. The users of herbal remedies had a significantly older age than nonusers, but no other significant differences were observed. The most prevalent complaints that led to herbal remedies use were gastroenterological complaints, flushing, and excessive thirst. Cichorium intybus, Borage officinalis, Mentha longifolia, and Matricaria recutita were the most prevalently used herbs in our patients. More study should be done on safety and efficacy of these herbs for hemodialysis patients. PMID:24241097

  5. Wild food plants used on the Dubrovnik coast (south-eastern Croatia

    Directory of Open Access Journals (Sweden)

    Katija Dolina

    2014-09-01

    Full Text Available Croatia’s versatile plant use traditions are still not sufficiently documented. The aim of this study was to record local traditions of wild food plant use on the Dubrovnik coast. We interviewed 40 inhabitants of 23 villages, mainly knowledgeable informants. On average 19 species were listed, which in total produced an inventory of 95 food plant species (including species whose leaves or inflorescences are used as recreational teas. The most commonly collected are: Sonchus oleraceus and S. asper, Asparagus acutifolius, Dioscorea communis, Cichorium intybus, Crepis zacintha, Allium ampeloprasum, Picris echioides and Foeniculum vulgare (all of them used as vegetables, the fruits of Rubus ulmifolius (mainly eaten raw, the fruits of roses (Rosa sempervirens and R. canina and the leaves of Salvia officinalis (both roses and salvia are used for making recreational teas. A particular feature of the local gastronomy is the collection of young Ruscus aculeatus shoots.

  6. Wild food plants used in the villages of the Lake Vrana Nature Park (northern Dalmatia, Croatia

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    Łukasz Łuczaj

    2013-12-01

    Full Text Available Croatia is a country of diverse plant use traditions, which are still insufficiently documented. The aim of this study was to document local traditions of using wild food plants around Lake Vrana (northern Dalmatia, Zadar region.  We interviewed 43 inhabitants of six traditional villages north of Lake Vrana. On average 12 species were listed, which in total produced an inventory of 55 food plants and 3 fungi taxa. Wild vegetables were most widely collected, particularly by older women who gathered the plants mainly when herding their flocks of sheep. Wild fruits and mushrooms were rarely collected. The former used to be an important supplementary food for children, or for everyone during times of food shortage, and the latter were relatively rare due to the dry climate and shortage of woods. The most commonly collected plants are wild vegetables: Cichorium intybus, Foeniculum vulgare, Sonchus oleraceus, Asparagus acutifolius, Papaver rhoeas, Rumex pulcher, Daucus carota, Allium ampeloprasum and Silene latifolia.

  7. Phytoremediation Opportunities with Alimurgic Species in Metal-Contaminated Environments

    Directory of Open Access Journals (Sweden)

    Marianna Bandiera

    2016-04-01

    Full Text Available Alimurgic species are edible wild plants growing spontaneously as invasive weeds in natural grassland and farmed fields. Growing interest in biodiversity conservation projects suggests deeper study of the multifunctional roles they can play in metal uptake for phytoremediation and their food safety when cultivated in polluted land. In this study, the responses of the tap-rooted perennial species Cichorium intybus L., Sonchus oleracerus L., Taraxacum officinale Web., Tragopogon porrifolius L. and Rumex acetosa L. were studied in artificially-highly Cd-Co-Cu-Pb-Zn-contaminated soil in a pot-scale trial, and those of T. officinale and R. acetosa in critical open environments (i.e., landfill, ditch sediments, and sides of highly-trafficked roads. Germination was not inhibited, and all species showed appreciable growth, despite considerable increases in tissue metal rates. Substantial growth impairments were observed in C. intybus, T. officinale and T. porrifolius; R. acetosa and S. oleracerus were only marginally affected. Zn was generally well translocated and reached a high leaf concentration, especially in T. officinale (~600 mg·kg−1·dry weight, DW, a result which can be exploited for phytoremediation purposes. The elevated Cd translocation also suggested applications to phytoextraction, particularly with C. intybus, in which leaf Cd reached ~16 mg·kg−1·DW. The generally high root retention of Pb and Cu may allow their phytostabilisation in the medium-term in no-tillage systems, together with significant reductions in metal leaching compared with bare soil. In open systems, critical soil Pb and Zn were associated with heavily trafficked roadsides, although this was only seldom reflected in shoot metal accumulation. It is concluded that a community of alimurgic species can serve to establish an efficient, long-lasting vegetation cover applied for phytoremediation and reduction of soil metal movements in degraded environments. However

  8. Dicty_cDB: Contig-U05635-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ( AL403593 ) T3 end of clone AT0AA009F07 of library AT0AA from... 64 8e-14 2 ( EE001710 ) ROE00012015 Rhizopus oryzae Company... CCIM10678.b1_L06.ab1 CCI(LMS) chicory Cichorium i... 56 2e-06 3 ( EE001897 ) ROE00012111 Rhizopus oryzae Company... EE004387 ) ROE00003982 Rhizopus oryzae Company Rhizopus oryz... 36 2e-06 4 ( GE315608 ) OXBA-aaa19b10.b1 Ox... ) CLPY4950.b1_L14.ab1 CLP(XYZ) lettuce perennis Lac... 48 9e-06 4 ( EC998752 ) ROE00010073 Rhizopus oryzae Company...376 ) ROE00010169 Rhizopus oryzae Company Rhizopus oryz... 36 0.061 3 ( CP000939 ) Clostridium botulinum B1

  9. Patogenicidade de Alternaria cichorii sobre espécies da família Asteraceae no Brasil

    Directory of Open Access Journals (Sweden)

    Lima Milton L. Paz

    2003-01-01

    Full Text Available Em 2001, plantas de chicória (Cichorium endivia originárias do município de Catalão-GO apresentando sintomas de manchas e queima foliar foram recebidas na Clínica Fitopatológica da Embrapa Hortaliças (CNPH. Tecidos submetidos à câmara úmida produziram esporulação fúngica, de onde foi obtida cultura monospórica em BDA + cloranfenicol. Para o teste de patogenecidade, o fungo foi multiplicado em meio de cultura suco de tomate ágar (ST e inoculado em plantas de chicória da cultivar comercial AG-3804, em casa-de-vegetação. Após dez dias de incubação, verificou-se nas plantas inoculadas a presença de sintomas, semelhantes àqueles observados inicialmente. O patógeno foi reisolado a partir destas lesões, completando os postulados de Koch. Em seguida, visando testar a possibilidade deste patógeno infetar outras plantas da família Asteraceae, conídios produzidos em ST e atomizados em plantas de alface (Lactuca sativa (três variedades, almeirão (Cichorium intybus (duas variedades, Catalonha (Cichorium intybus folha fina, serralha (Sonchus oleraceus e uma outra variedade de chicória. Houve infecção em todas as plantas inoculadas, sendo que os sintomas variaram nas espécies e nas variedades testadas. Em chicória os sintomas surgiram mais cedo e se desenvolveram rapidamente. O fungo foi caracterizado morfológica e morfometricamente permitindo identificá-lo como sendo Alternaria cichorii. Não se encontra registro desta espécie de Alternaria infetando plantas da família Asteraceae no Brasil. Neste trabalho relata-se pela primeira vez, a ocorrência de A. cichorii infetando chicória no Brasil e o potencial deste fungo como patógeno de outras espécies da família Asteraceae.

  10. Evaluation of Perennial Forage Legumes and Herbs in Six Mediterranean Environments Evaluación de Leguminosas y Hierbas Forrajeras Perennes en Seis Medioambientes Mediterráneos

    Directory of Open Access Journals (Sweden)

    Daniel Real

    2011-09-01

    Full Text Available There is an absence of drought tolerant herbaceous perennial forage legume and herb options other than lucerne (Medicago sativa L. for environments with Mediterranean-like climates common in extensive areas of Southern Australia, the Mediterranean basin, and Chile. Therefore, a collection of 174 forage perennial legume and herb entries from 103 species and 32 genera was evaluated for adaptation in a diverse range of Mediterranean climatic environments in Southern Australia. The seasonal rainfall distribution varied from moderately to highly winter dominant with long term average annual rainfall ranging from 318 to 655 mm. The entries were rated for productivity and persistence over 3 yr. The 12 entries identified as the most promising for winter, summer, or all-year round production included Bituminaria bituminosa (L. C.H. Stirt. var. albomarginata; Cichorium intybus L.; Cullen australasicum (Schltdl. J.W. Grimes; Dorycnium hirsutum (L. Ser.; Kennedia prostrata R. Br.; Lotononis bainesii Baker, Lotus pedunculatus Cav.; L. corniculatus L.; L. cytisoides L.; Medicago sativa subsp. sativa L.; Medicago sativa subsp. caerulea (Less. ex Ledeb. Schmalh., and M. sativa subsp. falcata (L. Arcang. These entries maintained production and persisted for the period of the evaluation, with the exception of C. intybus and L. corniculatus that declined in persistence over time. The potential role of these species in extensive grazing systems in Mediterranean climatic zones, their attributes and limitations, and current progress in developing them as useful forage plants was discussed.Existe una escasez de leguminosas y hierbas perennes herbáceas además de alfalfa (Medicago sativa L. tolerantes a sequía para ambientes con clima mediterráneo como los que se encuentran en el Sur de Australia, el Mediterráneo y Chile. Por lo tanto, una colección de 174 leguminosas perennes y hierbas correspondientes a 103 especies y 32 géneros fue evaluada por su adaptaci

  11. Actividad fitotóxica de un extracto N-Hexano obtenido de la corteza de Drimys Winteri sobre cuatro especies de malezas Phytotoxic activity of N-Hexane extract obtained from Drimys Winteri bark on four weeds

    Directory of Open Access Journals (Sweden)

    N. Zapata

    2011-06-01

    Full Text Available El objetivo de esta investigación fue determinar el efecto fitotóxico de un extracto obtenido con n-hexano de la corteza de Drimys winteri sobre la germinación y el crecimiento de Convolvulus arvensis, Setaria pumila, Daucus carota y Cichorium intybus. El efecto fitotóxico del extracto sobre la germinación de las malezas se determinó mediante bioensayos en placas de Petri y la aplicación del extracto en el medio de germinación en concentraciones de 100 a 1.000 mg L-1. En bioensayos en macetas, se determinó el efecto fitotóxico del extracto sobre la emergencia y crecimiento de las plántulas de malezas, en los cuales se aplicó el extracto mezclado con el sustrato de cultivo en concentraciones de 500 a 4.000 mg kg-1. También se determinó el efecto fitotóxico del extracto asperjado sobre plantas de malezas en concentraciones de 800 a 6.400 mg L-1. En los ensayos de germinación, el extracto retrasó la germinación y redujo el crecimiento de tallo y radícula de todas las malezas, el porcentaje de germinación disminuyó en todas las malezas, excepto en C. arvensis. La aplicación del extracto al sustrato de cultivo disminuyó y retrasó la emergencia de todas las malezas. Las plantas tratadas con extracto presentaron menor altura y hubo disminución de la biomasa acumulada. De igual modo, la aplicación del extracto al follaje de las malezas redujo la altura y la biomasa acumulada y llegó a causar la muerte de C. intybus, S. pumila y D. carota.The objective of this research was to determine the phytotoxic effect of the n-hexane extract obtained from the bark of Drimys winteri on germination and growth of Convolvulus arvensis, Setaria pumila, Daucus carota and Cichorium intybus. The phytotoxic effects of the extract on the germination of weeds were determined by bioassays in Petri dishes, adding the extract on the germination medium at concentrations of 100 to 1,000 mg L-1. The phytotoxic effects of the extract on the emergence and

  12. The effect of drought and interspecific interactions on depth of water uptake in deep- and shallow-rooting grassland species as determined by δ18O natural abundance

    Science.gov (United States)

    Hoekstra, N. J.; Finn, J. A.; Hofer, D.; Lüscher, A.

    2014-08-01

    Increased incidence of drought, as predicted under climate change, has the potential to negatively affect grassland production. Compared to monocultures, vertical belowground niche complementarity between shallow- and deep-rooting species may be an important mechanism resulting in higher yields and higher resistance to drought in grassland mixtures. However, very little is known about the belowground responses in grassland systems and increased insight into these processes may yield important information both to predict the effect of future climate change and better design agricultural systems to cope with this. This study assessed the effect of a 9-week experimental summer drought on the depth of water uptake of two shallow-rooting species (Lolium perenne L. and Trifolium repens L.) and two deep-rooting species (Cichorium intybus L. and Trifolium pratense L.) in grassland monocultures and four-species mixtures by using the natural abundance δ18O isotope method. We tested the following three hypotheses: (1) drought results in a shift of water uptake to deeper soil layers, (2) deep-rooting species take up a higher proportion of water from deeper soil layers relative to shallow-rooting species, and (3) as a result of interspecific interactions in mixtures, the water uptake of shallow-rooting species becomes shallower when grown together with deep-rooting species and vice versa, resulting in reduced niche overlap. The natural abundance δ18O technique provided novel insights into the depth of water uptake of deep- and shallow- rooting grassland species and revealed large shifts in depth of water uptake in response to drought and interspecific interactions. Compared to control conditions, drought reduced the proportional water uptake from 0-10 cm soil depth (PCWU0-10) of L. perenne, T. repens and C. intybus in monocultures by on average 54%. In contrast, the PCWU0-10 of T. pratense in monoculture increased by 44%, and only when grown in mixture did the PCWU0-10 of T

  13. In vitro anti-parasitic effects of sesquiterpene lactones from chicory against cattle nematodes

    DEFF Research Database (Denmark)

    Pena-Espinoza, Miguel Angel; Williams, A.; Boas, Ulrik;

    /water. Resulting extracts were incubated with cellulase enzymes, recovered in ethyl acetate and purified by normal solid-phase extraction. Obtained extracts were dissolved in 100% dimethyl sulfoxide (DMSO). A calf infected with O. ostertagi served as donor of nematode eggs. Eggs were hatched and L1 obtained were...... incubated in 8 extract concentrations for each cultivar (in duplicates) ranging from 2000 μg to 16 μg dry matter (DM) extract/ml (final concentration 1% DMSO in phosphate buffered saline-PBS). Ivermectin (1 mg/ml) and 1% DMSO in PBS were used as positive and negative controls, respectively. Viability of L1...

  14. In vitro anti-parasitic effects of sesquiterpene lactones from chicory against cattle nematodes

    DEFF Research Database (Denmark)

    Pena-Espinoza, Miguel Angel; Williams, A.; Boas, Ulrik;

    extracted in methanol/water. Resulting extracts were incubated with cellulase enzymes, recovered in ethyl acetate and purified by normal solid-phase extraction. Obtained extracts were dissolved in 100% DMSO. A calf infected with O. ostertagi served as donor of nematode eggs. Eggs were hatched and L1...... obtained were incubated in 8 extract concentrations for each cultivar (in duplicates) ranging from 2000 μg to 16 μg dry matter (DM) extract/mL (final concentration 1% DMSO in PBS). Ivermectin (1 mg/mL) and 1% DMSO in PBS were used as positive and negative controls, resp. Viability of L1 was evaluated...

  15. Regulation of Porcine Hepatic Cytochrome P450 by Chicory Root - Implication of Boar Taint

    OpenAIRE

    Rasmussen, Martin Krøyer

    2012-01-01

    Dietary components have been shown to affect the hepatic detoxification system. Hepatic clearance of xenobiotics is performed in two steps, Phase I and II. Phase I is usually carried out by enzymes belonging to the cytochrome P450 (CYP) family, while Phase II is carried out by a more diverse group. Phase I and II enzymes are extensively studied for their importance in drug clearance and in pigs for their impact on the occurrence of boar taint. It is generally believed that sufficient Phase I ...

  16. Sunn hemp with chicory or pearl millet to minimize gastrointestinal nematode infection in weaned goats

    Science.gov (United States)

    Predominantly grass forage systems are typically used throughout the southeastern U.S., but are inadequate for nutritional needs of growing goats, and encourage problems with gastrointestinal nematodes (GIN). Browse predominant forages would be preferable, but are not always available. Selection o...

  17. 菊苣的栽培技术%Planting Technology of Chicory

    Institute of Scientific and Technical Information of China (English)

    许生琴

    2003-01-01

    @@ 菊苣,为菊科菊苣属二年生或多年生草本植物,起源于地中海.菊苣的茎叶苦味较浓,栽培上需将其软化后方可食用,经软化后的菊苣叶,苦味减弱,且生香气,是供生食的高档色拉菜.2001年西宁市引进菊苣获得成功,产量达1250~1500千克.

  18. Finishing meat goats on birdsfoot trefoil, chicory, or red clover pastures

    Science.gov (United States)

    Control of gastrointestinal (GI) parasites in goats is a major challenge for producers. Some forages may contain natural compounds that can help in GI parasite control. This experiment was conducted to evaluate forage production patterns, animal performance, and health when meat goat kids were fin...

  19. Analysis of Heavy Metal Pollution of Lead, Iron Manganese,Zinc, Copper, and Chromium in Five Forage Grasses%5种牧草中铅、铬、锌、铜、铁、锰重金属污染的分析

    Institute of Scientific and Technical Information of China (English)

    张娜; 曹社会; 宋晓芳; 胡澍; 邓红侠

    2012-01-01

    The alfalfa (Medicago sativa Linn. ) , white clover (Trifolium repens Linn. ), chicory (Ci-chorium endivia Linn. ), barley ( Hordeum vulgare Linn. ) and smooth bromegrass (Bromus inermis Ley-ss. ) in Guanzhong plain were selected to determine the heavy metal content of lead, iron, manganeses, zinc, copper and chromium in shoots and roots of the five forages so as to assess the transfer capacity of heavy metal and the security of the forage. The results showed that; (1) there were differences among 6 heavy metal contents in 5 forages and there was a trend that the grasses are stronger than the legumes in absorption capacity of lead, iron, manganeses, zinc, copper and chromium . (2) The transfer coefficients of white clover to iron ,copper and chicory in manganeses, zinc and copper were bigger than 1, which were 1.70,2.55 and 1. 57 respectively,and it belongs to cumulative type. (3)The lead content of chicory and chromium content of five forages exceeded the standards for feeds; if chicory was used as a vegetable or barley as food grain, both of them did not meet the permitted amount of heavy metals, but if used as feed materials, they approached basically the corresponding standards.%论文测定了陕西关中地区(霋)土紫花苜蓿(Medicago sativa Linn.)、白三叶(Trifolium repens Linn.)、菊苣(Cichorium endivia Linn.)、大麦(Hordeum vulgare Linn.)和无芒雀麦(Bromus inermis Leyss.)地上部与根部铅、铬、锌、铜、铁、锰5种重金属元素的含量,评价了参试牧草对各种重金属的转运能力和饲草的安全性.结果表明:(1)5种牧草根部对所测六种重金属元素的吸收转运能力不同,对铅、铁、锰、锌、铜、铬的吸收能力有禾本科强于豆科的趋势;(2)白三叶对于铁,铜和菊苣对于猛、锌、铜的转运系数大于1,分别为1.70、2.55、1.57,属富积型植物;(3)菊苣的铅含量与种牧草的铬含量均已超过饲料标准;菊苣和大麦作为人类的蔬菜和粮食均

  20. Evaluation of Effects of Zingiber officinale on Salivation in Rats

    Directory of Open Access Journals (Sweden)

    Goli Chamani

    2011-06-01

    Full Text Available There are some herbal plants in Iranian traditional system of medicine which are believed to be excellent remedies to alleviate the symptoms of xerostomia. The aim of the present study was to evaluate the effect of systemic administration of seven different herbal extracts on the rate of salivation in rats. The extracts of 7 herbs; Zingiber officinale Roscoe (Zingiberaceae, Citrus sinensis (L. Osbeck (Rutaceae, Artemisia absinthium L. (Asteraceae, Cichorium intybus L. (Asteraceae, Pimpinella anisum L.(Apiaceae, Portulaca oleracea L.(Portulacaceae, Tribulus terrestris L. (Zygophyllaceae were prepared. Nine groups of animals (including negative and positive control groups were used and seven rats were tested in each group. After the injection of extracts, saliva volume was measured gravimetrically in four continuous seven-minute intervals. The results showed that after injection of ginger extracts salivation was significantly higher as compared to the negative control group and other herbal extracts in all of the four intervals (P<0.01. The peak action of the ginger was during the first 7-minute interval and following this, salivation decreased to some extent. The present study suggests that the extract of Zingiber offiicianle can increase the rate of salivation significantly in animal model. Further investigations on different constituents of ginger seem to be essential to identify the responsible constituent for stimulation of saliva secretion.

  1. Liver tonics:review of plants used in Iranian traditional medicine

    Institute of Scientific and Technical Information of China (English)

    Tahmineh; Akbarzadeh; Reyhaneh; Sabourian; Mina; Saeedi; Hossein; Rezaeizadeh; Mahnaz; Khanavi; Mohammad; Reza; Shams; Ardekani

    2015-01-01

    Considering the fact that liver is one of the most important organs in our body,it deserves special attention and protection.Among various recommended supplements,complementary and alternative medicines particularly herbal remedies have received much attention owing to their truly healing properties.This review profits from Iranian traditional medicine and presents advantageous herbal guide directions for liver protection.According to credible Iranian medical literature such as Al Qanun Fil Tibb.Al-Havi and Makhzan-al-Aadvia.a wide spectrum of plants have been found to be useful for cleansing and protecting the liver.Some herbs such as ghafes(Agrimonia eupatoria),kasni(Cichorium intybus),anar(Punica granatum),darchin(Cinnamomum zeylanicum),za’feran(Crocus sativus),gole-sorkh(Rosa damascena) and zereshk(Berberis vulgaris) appeared to get strong consideration and were well documented as outstanding liver tonics.We conducted a comprehensive review of available Iranian medical resources such as scientific information database and medical sciences databases which cover all in vitro and in vivo studies of medicinal plants as liver tonics and hepatoprotcctive candidates.Literature survey was accomplished using multiple databases including PubMed,ISI web of knowledge,and Google Scholar.

  2. Prebiotic effect of Agave fourcroydes fructans: an animal model.

    Science.gov (United States)

    García-Curbelo, Yanelys; Bocourt, Ramón; Savón, Lourdes L; García-Vieyra, Maria Isabel; López, Mercedes G

    2015-09-01

    The use of prebiotics such as fructans has increased in human and animal nutrition because of their productive performance and health benefits. Agave fourcroydes has shown high concentrations of fructans in their stems; however, there is no information on new products derived from this plant that might enhance its added value. Therefore, we evaluated the prebiotic effect of Agave fourcroydes fructans in an animal model. Male mice (C57BL/6J) were fed on parallel form with a standard diet or diets supplemented with 10% of fructans from Cichorium intybus (Raftilose P95) and Agave fourcroydes from Cuba for 35 days. The body weight, food intake, blood glucose, triglycerides and cholesterol, gastrointestinal organ weights, fermentation indicators in cecal and colon contents and mineral content in femurs were determined. The body weight and food intake of mice were not significantly modified by any treatment. However, serum glucose, cholesterol and triglycerides decreased (P Agave fourcroydes in the mice diet induced a prebiotic response, similar to or greater than the commercial product (Raftilose P95) and this constitutes a promising alternative with potential use not only in animal but also in human diets. PMID:26237650

  3. Evaluation of effects of Zingiber officinale on salivation in rats.

    Science.gov (United States)

    Chamani, Goli; Zarei, Mohammad Reza; Mehrabani, Mitra; Taghiabadi, Yousef

    2011-01-01

    There are some herbal plants in Iranian traditional system of medicine which are believed to be excellent remedies to alleviate the symptoms of xerostomia. The aim of the present study was to evaluate the effect of systemic administration of seven different herbal extracts on the rate of salivation in rats. The extracts of 7 herbs; Zingiber officinale Roscoe (Zingiberaceae), Citrus sinensis (L.) Osbeck (Rutaceae), Artemisia absinthium L. (Asteraceae), Cichorium intybus L. (Asteraceae), Pimpinella anisum L.(Apiaceae), Portulaca oleracea L.(Portulacaceae), Tribulus terrestris L. (Zygophyllaceae) were prepared. Nine groups of animals (including negative and positive control groups) were used and seven rats were tested in each group. After the injection of extracts, saliva volume was measured gravimetrically in four continuous seven-minute intervals. The results showed that after injection of ginger extracts salivation was significantly higher as compared to the negative control group and other herbal extracts in all of the four intervals (P<0.01). The peak action of the ginger was during the first 7-minute interval and following this, salivation decreased to some extent. The present study suggests that the extract of Zingiber offiicianle can increase the rate of salivation significantly in animal model. Further investigations on different constituents of ginger seem to be essential to identify the responsible constituent for stimulation of saliva secretion. PMID:21874635

  4. The melliferous potential of apiflora of southwestern Vojvodina (Serbia

    Directory of Open Access Journals (Sweden)

    Mačukanović-Jocić Marina P.

    2016-01-01

    Full Text Available The individual and community-level melliferous potential of apiflora was evaluated in southwestern Vojvodina in order to assess its significance and contribution to the bee pasture. Seven plant communities belonging to ruderal, segetal and floodplain type of vegetation, with a total of 279 plant species were registered. Apifloristic and phytocoenological investigations included the determination and analysis of honey plants using the following parameters: total number, percentage, abundance and frequency of these species in the communities, as well as their intensity of pollen and nectar production. The coenotic coefficient of melliferousness (CCm indicating the melliferous potential of each community, was calculated based on the above parameters. Although the greatest number of melliferous species was found in the ass. Chenopodio-Ambrosietum artemisiifoliae (132, the highest percentage (80% of them was registered in the ass. Consolido-Polygonetum avicularis. Considering the coefficients of nectar and pollen production, the most valuable honey plants commonly present in the majority of communities were: Cirsium arvense, Rubus caesius, Lythrum salicaria, Daucus carota, Trifolium pratense, Dipsacus laciniatus, Medicago sativa, Asclepias syriaca, Cichorium intybus and Taraxacum officinale. The low abundance and frequency of melliferous species within the Consolido-Polygonetum avicularis, Polygonetum convolvulo-avicularis and Populetum nigrae-albae communities indicated their poor contribution to the bee pasture. Within ruderal vegetation, the highest CCm was registered in Amorpho-Typhaetum, providing, theoretically, the richest food resource for the honeybees in the investigated area. [Projekat Ministarstva nauke Republike Srbije, br. 46009 i br. 173018

  5. Asteretum lanceolati: Xenospontaneous community on wet and riparian habitats

    Directory of Open Access Journals (Sweden)

    Obratov-Petković Dragica

    2011-01-01

    Full Text Available Invasive species Aster lanceolatus grows on moist habitats on the whole territory of Serbia. In Belgrade, this species is recorded with a higher degree of presence at a number of localities. With the aim to investigate the community in which this species is dominant, the wide area of Serbia was researched, and 8 localities on the territory of Belgrade were chosen for the analysis of the community. Floristic structure of the community was determined by the standard Braun-Blanquet method (1964, phytogeographical analysis was performed according to Gajić (1980, 1984, and determination of life forms according to Raunkier (Ellenberg, Mueller-Dombois, 1967. pH soil analysis and electric conductivity (EC were performed at all investigated localities. It was established that the community dominates the moist habitats of Belgrade. It is composed of 104 species and among them Aster lanceolatus Willd., Cichorium intybus L., Agropyrum repens (L. Beauv., Calystegia sepium (L. R. Br., Cirsium arvense (L. Scop., Symphytum officinale L. and Rumex obtusifolius L. are the most frequent. In relation to life forms, the community has hemicriptophytes character, and in relation to phytogeography Euroasian and Middle Europaean floral elements are dominant, with a high presence of cosmopolitan and adventive floral elements. On the locialities Veliko Ratno ostrvo (island and Makiš, EC values point to the fact that the amount of nutrient in the soil is higher than at other localities.

  6. Pesticide residues in some herbs growing in agricultural areas in Poland.

    Science.gov (United States)

    Malinowska, Elżbieta; Jankowski, Kazimierz

    2015-12-01

    The aim of this paper was to assess residue content of plant protection products in selected herbs: Achillea millefolium L., Cichorium intybus L., Equisetum arvense L., Polygonum persicaria L., Plantago lanceolata L., and Plantago major L. The study comprises herbs growing in their natural habitat, 1 and 10 m away from crop fields. The herbs, 30 plants of each species, were sampled during the flowering stage between 1 and 20 July 2014. Pesticide residue content was measured with the QuECHERS method in the dry matter of leaves, stalks, and inflorescence, all mixed together. Out of six herb species growing close to wheat and maize fields, pesticide residues were found in three species: A. millefolium L., E. arvense L., and P. lanceolata L. Most plants containing the residues grew 1 m away from the wheat field. Two active substances of fungicides were found: diphenylamine and tebuconazole, and one active substance of insecticides: chlorpyrifos-ethyl. Those substances are illegal to use on herbal plants. Samples of E. arvense L. and P. lanceolata L. contained two active substances each, which constituted 10% of all samples, while A. millefolium L. contained one substance, which is 6.6% of all samples. PMID:26612566

  7. Palatability of weeds from different European origins to the slugs Deroceras reticulatum Müller and Arion lusitanicus Mabille

    Science.gov (United States)

    Keller, Michael; Kollmann, Johannes; Edwards, Peter J.

    1999-04-01

    As part of a study on the significance of seed provenances in schemes to enhance biodiversity in agricultural habitats, juvenile plants of Cichorium intybus, Daucus carota, Leucanthemum vulgare and Silene alba of different European origins were exposed to grazing by two slug species, Deroceras reticulatum and Arion lusitanicus. Living plants were offered in trays, either in a glasshouse ( Deroceras) or outdoors ( Arion). The amount of herbivory was origin-dependent, with higher losses for all four species from German and Hungarian provenances compared with English and Swiss plants. The main trend was similar for both slug species except in the case of Daucus, and there was a significant 'origin × plant species' interaction. We found strong correlations between provenance-specific herbivory and certain climatic characteristics of the corresponding regions, i.e. winter minimum temperatures, and dryness in spring and late summer, which are crucial for the development of slugs. The results can be interpreted in terms of a SW-NE European climatic gradient and may be a consequence of differences in the need for plant defences against herbivory by slugs. Additionally, the data on palatability were compared with susceptibility towards two parasites which occurred in a field experiment, a leaf miner on Leucanthemum vulgare and a rust fungus on Silene alba. While specific leaf mining frequencies on Leucanthemum contrasted with the palatability of the different provenances to slugs, the rust infection on Silene was low on local and German plants, and higher on the more distant provenances from England and Hungary.

  8. Antihyperglycemic effect of crude extracts of some Egyptian plants and algae.

    Science.gov (United States)

    AbouZid, Sameh Fekry; Ahmed, Osama Mohamed; Ahmed, Rasha Rashad; Mahmoud, Ayman; Abdella, Ehab; Ashour, Mohamed Badr

    2014-03-01

    Diabetes mellitus is a major global health problem. Various plant extracts have proven antidiabetic activity and are considered as promising substitution for antidiabetic drugs. The antihyperglycemic effect of 16 plants and 4 algae, commonly used in Egypt for the treatment of diabetes mellitus, was investigated. A diabetes model was induced by intraperitoneal injection of nicotinamide (120 mg/kg body weight [b.wt.]), then streptozotocin (200 mg/kg b.wt.) after 15 min. Hydroethanolic extracts (80%) of the plants and algae under investigation were prepared. The extracts were orally administered to nicotinamide-streptozotocin-induced diabetic mice by a gastric tube at doses 10 or 50 mg/kg b.wt. for 1 week. The antidiabetic activity was assessed by detection of serum glucose concentrations at the fasting state and after 2 h of oral glucose loading (4.2 mg/kg b.wt.). Extracts prepared from Cassia acutifolia, Fraxinus ornus, Salix aegyptiaca, Cichorium intybus, and Eucalyptus globulus showed the highest antihyperglycemic activity among the tested plants. Extracts prepared from Sonchus oleraceus, Bougainvillea spectabilis (leaves), Plantago psyllium (seeds), Morus nigra (leaves), and Serena repens (fruits) were found to have antihyperglycemic potentials. Extracts prepared from Caulerpa lentillifera and Spirulina versicolor showed the most potent antihyperglycemic activity among the tested algae. However, some of the tested plants have insulinotropic effects, all assessed algae have not. Identification of lead compounds from these plants and algae for novel antidiabetic drug development is recommended. PMID:24404976

  9. Ethno-medicinal plants used to cure jaundice by traditional healers of mashhad, iran.

    Science.gov (United States)

    Amiri, Mohammad Sadegh; Joharchi, Mohammad Reza; Taghavizadehyazdi, Mohammad Ehsan

    2014-01-01

    Jaundice is the commonest ailments affecting the citizens of both developed and poor Asians countries including Iran. An ethnobotanical survey of plants used by the traditional healers for the treatment of jaundice was conducted in the Mashhad city, Northeastern Iran. A total of 37 plants belonging to 32 genera and 26 families have been documented for their therapeutic use against jaundice. The plant families which contained the most commonly used species for their effects are: Fabaceae (5 species), Polygonaceae (4 sp.), Asteraceae (3 sp.), Plantaginaceae (2 sp.) and Salicaceae (2 sp.). The plants were arranged with correct nomenclature along with their common name, family, the part used and their medicinal value. The use of decoction is the most preferred method of herbal preparation. In all cases, the treatment involved oral administration of the extracts 2 to 3 times daily from a week to month till the problem disappears. Cichorium intybus, Salix alba, Cotoneaster nummularius, Descurainia sophia, Malva sylvestris, Berberis integrrima, Rumex acetosella, Phyllanthus emblica and Alhagi maurorum were repeatedly mentioned by the traditional healers as the most widely used for the treatment of jaundice in the study area. The study indicates that the local inhabitants rely on medicinal plants for treatment. This paper suggested that further clinical experimentation is needed to scientifically evaluate these widely used herbal remedies for possible bioactive effects. PMID:24734067

  10. Vegetable and animal food sorts found in the gastric content of Sardinian Wild Boar (Sus scrofa meridionalis).

    Science.gov (United States)

    Pinna, W; Nieddu, G; Moniello, G; Cappai, M G

    2007-06-01

    Authors report results emerging from gastric content analysis from n. 96 wild boars hunted in Sardinia isle, during the hunting tide (2001-2005), from November to January. Mean pH of the gastric content was 3.77 +/- 0.69. Mean total capacity (TC) of each stomach was 1702 +/- 680 g. Mean Stuff ratio (CW/TC) between the content weight (CW) and stomachs TC was 0.45. Food categories found in animal stomachs were: 19 categories of vegetal species (Allium spp., Arbutus unedo, Arisarum vulgare, Avena fatua, Avena sativa, Castanea sativa, Ceratonia siliqua, Chamaerops umilis, Cichorium intybus, Hordeum sativum, Juniperus oxycedrus, Myrtus communis, Olea europea, Pirus amygdaliformis, Pistacia lentiscus, Quercus spp., Rhamnus alaternus, Triticum durum, Zea mais); 11 categories of animal species (Agriotes lineatus, Apodemus sylvaticus dicrurus, Chalcides chalcides, Chalcides ocellatus tiligugu, Crematogaster scutellaris, Forficula auricularia, Helix aspersa, Lumbricus terrestris, Ovis aries, Podarcis tiliguerta tiliguerta, Scolopendra cingulata); three categories were identified in general terms (insects larvae, hairs of mammals, feathers of birds). Food categories found in the stomach contents of Sus scrofa meridionalis confirm observations by other researchers who report the prevalence of vegetables in spite of animal food sorts in the wild boar diet in Italian regions. PMID:17516948

  11. POSSIBILITIES TO USE NATURAL EXTRACTS FROM MEDICINAL AND AROMATIC PLANTS (MAP LIKE BOTANICAL REPELLENT OR INSECTICIDE COMPOUNDS AGAINST PEST INSECTS IN ECOLOGICAL CROPS (II

    Directory of Open Access Journals (Sweden)

    Irina IONESCU-MĂLĂNCUŞ

    2013-12-01

    Full Text Available Botanical insecticides have long been touted as attractive alternatives to synthetic chemical insecticides for pest management because botanicals reputedly pose little threat to the environment or to human health. The body of scientific literature documenting bioactivity of plant derivatives to arthropods pests continues to expand i.e. repellents based on essential oils extracted from Chenopodium ambrosioides, Eucalyptus saligna, Rosmarinus officinalis to mosquitoes, or cinnamon oil, sandalwood oil and turmeric oil are previously reported as insect repellents evaluatede in the laboratory conditions. With the constantly increasing problems of insecticide resistance and increasing public concerns regarding pesticide safety, new, safer active ingredients are becoming necessary to replace existing compounds on the market. The present study carried out in the period 2010-2012 comprises a review of two insect repellents, followed by some new research conducted in our laboratory on plant-derived insect repellents. The two alkaloids tested against the Colorado potato beetle, Leptinotarsa decemlineata Say in laboratory conditions was obtained by water and alchohol extraction from two vegetal species, Cichorium intybus L. (Asterales:Asteraceae and Delphinium consolida L. (Ranales:Ranunculaceae. The tests carried out in laboratory and field experimentally plots under cages permit to evaluate several other compounds for repellent activity of lacctucin alkaloids.

  12. ELABORATION OF APPROACHES TO THE STANDARTIZ OFATION HELICHRYSUM ARENARIUM (1. MOENCH IN PLANT MIXTURES

    Directory of Open Access Journals (Sweden)

    A. B. Goudzenko

    2014-01-01

    Full Text Available Marker for qualitative and quantitative standardization of Helichrysum arenarium (L. Moench. flowers in the plant mixtures was determine. As a marker of Helichrysum arenarium Moench. flowers can be used flavonoid apigenin. HPLC method of determination flavonoid apigenin in raw materials and plant mixtures of flowers Helichrysum arenarium (L. Moench was developed. Contents of apigenin in the raw plant flowers were in the range from 0,1453 ± 0,0068% to 0,1657 ± 0,0085%, in terms of the dried materials. It is shown, that the presence and content of apigenin flowers Helichrysum arenarium (L. Moench can be standardized in the mixtures with the following plant:flowers, leaves and fruits of genus Crataegus L., roots of Althaea officinalis L., roots of Glycyrrhiza glabra L., roots of Cichorium intybus L., roots of Taraxacum officinale Web., cones of Humulus lupulus L., herb of Leonurus cardiaca L., herb of Hypericum perforatum L. and seeds of Linum usitatissimum L.

  13. Toxicological assessment of P-9801091 plant mixture extract after chronic administration in CBA/HZg mice--a biochemical and histological study.

    Science.gov (United States)

    Petlevski, Roberta; Hadzija, Mirko; Slijepcević, Milivoj; Juretić, Dubravka

    2008-06-01

    Acute, subchronic and chronic effects of the P-9801091 plant mixture extract at a dose of 20 mg/kg body mass were assessed in serum of healthy CBA/HZg mice at 24 hours, 7 days, 3 months and 6 months of treatment (experimental group), and compared with the values obtained in the control group of untreated healthy CBA/HZg mice. The P-9801091 plant mixture extract is an antihyperglycemic preparation containing Myrtilli folium (Vaccinium myrtillus L.), Taraxaci radix (Taraxacum officinale Web.), Cichorii radix (Cichorium intybus L.), Juniperi fructus (Juniperus communis L.), Centaurii herba (Centaurium umbellatum Gilib.), Phaseoli fructus sine semine (Phaseolus vulgaris L.), Millefolii herba (Achillea millefolium L.), Mori folium (Morus nigra L.), Valerianae radix (Valeriana officinalis L.) and Urticae herba et radix (Urtica dioica L). Toxic effect of the P-9801091 plant mixture extract was assessed by the following biochemical parameters: urea, creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and cholesterol. Also, histopathological examination of the kidneys, liver, spleen, pancreas, testes and lungs was performed. Results of biochemical testing performed at specified time points generally showed no statistically significant differences from control values, with the only exception of the catalytic concentration of AST in the experimental group measured on day 7, which was significantly increased as compared with the control group (p<0.05). Pathohistological examination including characteristic organ and tissue structure, and parenchyma relationship to the adjacent blood vessels and connective tissue in the examined organs revealed no major pathologic changes. PMID:18756913

  14. Antioxidant characterization of some Sicilian edible wild greens.

    Science.gov (United States)

    Salvatore, Sara; Pellegrini, Nicoletta; Brenna, Oreste V; Del Rio, Daniele; Frasca, Graziella; Brighenti, Furio; Tumino, Rosario

    2005-11-30

    Epidemiological studies have demonstrated that many antioxidants and the total antioxidant capacity (TAC) of the diet may protect against cancers and cardiovascular disease. Common fruits and vegetables are good sources of antioxidants, although in some Mediterranean areas traditional wild greens are responsible for a significant percentage of total dietary antioxidant intake. In the European Prospective Investigation into Cancer and Nutrition cohort of Ragusa (Sicily), a high number of subjects were found to frequently eat wild greens, including Sinapis incana and Sinapis nigra, Diplotaxis erucoides, Cichorium intybus, Asparagus acutifolius, and Borrago officinalis. On the basis of these observations, detailed characterization of single antioxidant components (i.e., polyphenols, carotenoids, chlorophylls, and ascorbic acid) and the TAC of these edible wild traditional plants was performed. The wild plants examined were found to be very rich in antioxidants, such as flavonoids and carotenoids, with high TAC values, suggesting that the importance of these vegetables, not only in the traditional but even in the contemporary diet, needs to be emphasized. PMID:16302763

  15. In vitro antioxidant activity of non-cultivated vegetables of ethnic Albanians in southern Italy.

    Science.gov (United States)

    Pieroni, A; Janiak, V; Dürr, C M; Lüdeke, S; Trachsel, E; Heinrich, M

    2002-08-01

    A total of 27 extracts from non-cultivated and weedy vegetables traditionally consumed by ethnic Albanians (Arbëreshë) in the Vulture area (southern Italy) were tested for their free radical scavenging activity (FRSA) in the DPPH (1,1-diphenyl-2-picrylhydrazil radical) screening assay, for their in vitro non-enzymatic inhibition of bovine brain lipid peroxidation and for their inhibition of xanthine oxidase (XO). In both antioxidant assays strong activity was shown for Leopoldia comosa (bulbs, syn.: Muscari comosum) and Centaurea calcitrapa (young whorls). In the lipid peroxidation assay, extracts from leaves of Origanum heracleoticum, Urtica dioica and Tordylium apulum showed a remarkable inhibitory activity (> 50%), too. In the case of Leopoldia comosa and Origanum heracleoticum this activity was comparable to quercetin (at a concentration of 50 microM) and Rhodiola rosea extract. Extracts from non-cultivated Cichorium intybus, Chondrilla juncea and Stellaria media showed strong in vitro inhibition of xanthine oxidase, with an activity higher than that of a reference extract from Ledum groenlandicum. These findings suggest that weedy vegetables may be useful antioxidants of interest in the prevention of ageing related diseases, CNS disorders and as potential sources of phytomedicines against hyperuricaemia and gout. PMID:12203269

  16. Pasture intake and milk production of dairy cows rotationally grazing on multi-species swards.

    Science.gov (United States)

    Roca-Fernández, A I; Peyraud, J L; Delaby, L; Delagarde, R

    2016-09-01

    Increasing plant species diversity has been proposed as a means for enhancing annual pasture productivity and decreasing seasonal variability of pasture production facing more frequent drought scenarios due to climate change. Few studies have examined how botanical complexity of sown swards affects cow performance. A 2-year experiment was conducted to determine how sward botanical complexity, from a monoculture of ryegrass to multi-species swards (MSS) (grasses-legumes-forb), affect pasture chemical composition and nutritive value, pasture dry matter (DM) intake, milk production and milk solids production of grazing dairy cows. Five sward species: perennial ryegrass (L as Lolium), white clover and red clover (both referred to as T as Trifolium because they were always sown together), chicory (C as Cichorium) and tall fescue (F as Festuca) were assigned to four grazing treatments by combining one (L), three (LT), four (LTC) or five (LTCF) species. Hereafter, the LT swards are called mixed swards as a single combination of ryegrass and clovers, whereas LTC and LTCF swards are called MSS as a combination of at least four species from three botanical families. The experimental area (8.7 ha) was divided into four block replicates with a mineral nitrogen fertilisation of 75 kg N/ha per year for each treatment. In total, 13 grazing rotations were carried out by applying the same grazing calendar and the same pasture allowance of 19 kg DM/cow per day above 4 cm for all treatments. Clover represented 20% of DM for mixed and MSS swards; chicory represented 30% of DM for MSS and tall fescue represented 10% of DM for LTCF swards. Higher milk production (+1.1 kg/day) and milk solids production (+0.08 kg/day) were observed for mixed swards than for ryegrass swards. Pasture nutritive value and pasture DM intake were unaffected by the inclusion of clover. Pasture DM, organic matter and NDF concentrations were lower for MSS than for mixed swards. Higher milk production (+0.8 kg

  17. GC-MS Analysis of Fatty Acid Composition of Wild Chicory Chicory Grown in Plastic Shed%棚栽与野生苣荬菜中脂肪酸的气相色谱-质谱分析

    Institute of Scientific and Technical Information of China (English)

    丁旭光; 侯冬岩; 关崇新; 刘晓媛

    2009-01-01

    对棚栽与野生苣荬菜中脂肪酸进行分析.采用索氏提取法提取棚栽与野生苣荬菜中脂肪酸,再进行甲酯化处理,以气相色谱-质谱联用仪分离和鉴定脂肪酸的组成和相对含量.结果表明:由棚栽苣荬菜中分离鉴定出10种脂肪酸,不饱和脂肪酸占30.87%;由野生苣荬菜中分离鉴定出7种脂肪酸,不饱和脂肪酸占57.3%.

  18. Evaluation of antibacterial activity of crude protein extracts from seeds of six different medical plants against standard bacterial strains.

    Science.gov (United States)

    Al Akeel, Raid; Al-Sheikh, Yazeed; Mateen, Ayesha; Syed, Rabbani; Janardhan, K; Gupta, V C

    2014-04-01

    A huge group of natural antimicrobial compounds are active against a large spectrum of bacterial strains causing infectious threat. The present study was conducted to investigate the crude extracts of antimicrobial protein and peptide efficacy from six medicinal plant seeds. Extraction was carried out in Sodium phosphate citrate buffer, and Sodium acetate buffer using different pH. Antimicrobial activities of these plants were determined by the microbiological technique using Agar well diffusion Assay. Extremely strong activity was observed in the seed extracts of Allium ascolinicum extracted in sodium phosphate citrate buffer at pH (5.8) against Proteus vulgaris, Escherichia coli and Staphylococcus aureus with zone of inhibition 17 mm, 17 mm and 15 mm and Rumex vesicarius at pH (7.6), Ammi majus at pH (6.8), Cichorium intybus at pH (7.4) and Cucumis sativus at pH (7.8) also showed better sensitivity against the bacterial strains with zone of inhibition ranges 16-10 mm and some of the strains were found to be resistant. Antibacterial activity pattern of different plant extracts prepared in sodium acetate buffer pH (6.5), among all the plant seed extracts used Foeniculum vulgare had shown good inhibition in all the bacterial strains used, with zone of inhibition ranges 11-12.5 mm, The extracts of C. intybus and C. sativus were found to be effective with zone of inhibition 11-6 mm and some of the strains were found to be resistant. Most of the strains found to have shown better sensitivity compared with the standard antibiotic Chloramphenicol (25 mcg). Our results showed that the plants used for our study are the richest source for antimicrobial proteins and peptides and they may be used for industrial extraction and isolation of antimicrobial compounds which may find a place in medicine industry as constituents of antibiotics. PMID:24600307

  19. Labelling plants the Chernobyl way: A new approach for mapping rhizodeposition and biopore reuse

    Science.gov (United States)

    Banfield, Callum; Kuzyakov, Yakov

    2016-04-01

    A novel approach for mapping root distribution and rhizodeposition using 137Cs and 14C was applied. By immersing cut leaves into vials containing 137CsCl solution, the 137Cs label is taken up and partly released into the rhizosphere, where it strongly binds to soil particles, thus labelling the distribution of root channels in the long term. Reuse of root channels in crop rotations can be determined by labelling the first crop with 137Cs and the following crop with 14C. Imaging of the β- radiation with strongly differing energies differentiates active roots growing in existing root channels (14C + 137Cs activity) from roots growing in bulk soil (14C activity only). The feasibility of the approach was shown in a pot experiment with ten plants of two species, Cichorium intybus L., and Medicago sativa L. The same plants were each labelled with 100 kBq of 137CsCl and after one week with 500 kBq of 14CO2. 96 h later pots were cut horizontally at 6 cm depth. After the first 137Cs + 14C imaging of the cut surface, imaging was repeated with three layers of plastic film between the cut surface and the plate for complete shielding of 14C β- radiation to the background level, producing an image of the 137Cs distribution. Subtracting the second image from the first gave the 14C image. Both species allocated 18 - 22% of the 137Cs and about 30 - 40% of 14C activity below ground. Intensities far above the detection limit suggest that this approach is applicable to map the root system by 137Cs and to obtain root size distributions through image processing. The rhizosphere boundary was defined by the point at which rhizodeposited 14C activity declined to 5% of the activity of the root centre. Medicago showed 25% smaller rhizosphere extension than Cichorium, demonstrating that plant-specific rhizodeposition patterns can be distinguished. Our new approach is appropriate to visualise processes and hotspots on multiple scales: Heterogeneous rhizodeposition, as well as size and counts

  20. Tissue Culture and Plant Regeneration of Chicory%菊苣组织培养及植株再生

    Institute of Scientific and Technical Information of China (English)

    杨春燕; 廖明安; 韩永芬; 唐成斌; 王小利

    2008-01-01

    以普那菊苣叶柄为外植体进行组织培养试验,结果表明:菊苣叶柄在5种培养基上均可诱导出愈伤组织,总出愈率在90%以上,但分化效果因培养基的不同而有较大的差异.诱导愈伤组织及分化的最佳培养基为:MS+KT 1.0 mg/L十NAA 0.2 mg/L;生根培养基为:MS+IBA 0.2 mg/L.