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Sample records for cherry cultivar improvement

  1. De novo transcriptome assembly of a sour cherry cultivar, Schattenmorelle

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    Yeonhwa Jo

    2015-12-01

    Full Text Available Sour cherry (Prunus cerasus in the genus Prunus in the family Rosaceae is one of the most popular stone fruit trees worldwide. Of known sour cherry cultivars, the Schattenmorelle is a famous old sour cherry with a high amount of fruit production. The Schattenmorelle was selected before 1650 and described in the 1800s. This cultivar was named after gardens of the Chateau de Moreille in which the cultivar was initially found. In order to identify new genes and to develop genetic markers for sour cherry, we performed a transcriptome analysis of a sour cherry. We selected the cultivar Schattenmorelle, which is among commercially important cultivars in Europe and North America. We obtained 2.05 GB raw data from the Schattenmorelle (NCBI accession number: SRX1187170. De novo transcriptome assembly using Trinity identified 61,053 transcripts in which N50 was 611 bp. Next, we identified 25,585 protein coding sequences using TransDecoder. The identified proteins were blasted against NCBI's non-redundant database for annotation. Based on blast search, we taxonomically classified the obtained sequences. As a result, we provide the transcriptome of sour cherry cultivar Schattenmorelle using next generation sequencing.

  2. Bioactivity of sour cherry cultivars grown in Denmark

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    Khoo, Gaik Ming; Clausen, Morten Rahr; Pedersen, Bjarne Hjelmsted;

    2012-01-01

    Thirty four varieties of sour cherries (Prunus cerasus) were investigated for their total antioxidant activity, Caco-2 cancer cell proliferation inhibitory activity and effect on prostaglandin E2 (PGE2) production. Total phenolic content, oxygen radical absorbance capacity (ORAC) and cancer cell...... proliferation inhibitory activity of sour cherries were closely correlated but not PGE2 production. The cultivars ‘BirgitteBöttermö’, ‘Fanal’ and ‘Tiki’ were the three cultivars with the highest ORAC values (180, 147 and 133mmol TE/g, respectively) and inhibition against Caco-2 cancer cell proliferation (74...

  3. Influence of cultivar and processing on cherry (Prunus avium) allergenicity.

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    Primavesi, L; Brenna, O V; Pompei, C; Pravettoni, V; Farioli, L; Pastorello, E A

    2006-12-27

    Oral allergy syndrome is an immediate food allergic event that affects lips, mouth, and pharynx, is often triggered by fruits and vegetables, and may be associated with pollinosis. Here, we report on the allergenic pattern of different varieties of cherry (Prunus avium) and results obtained by applying several technological processes to the selected varieties. Whole cherries were submitted to chemical peeling, thermal treatment, and syruping processes, and the relative protein extracts were analyzed by in vitro (sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting analysis) and in vivo tests (skin prick test). Electrophoretic analyses demonstrated that there was no marked difference among cherry cultivars. Chemical peeling successfully removed Pru av 3, a lipid transfer protein (LTP) responsible for oral allergy syndrome in patients without pollinosis, leading to the industrial production of cherry hypoallergenic derivatives. Furthermore, the syruping process removed almost all allergenic proteins to whom patients with pollinosis are responsive. In vivo tests confirmed electrophoretic results.

  4. Cultivar effect on the sweet cherry antioxidant and some chemical attributes

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    Skrzyński Jan

    2016-06-01

    Full Text Available This research was carried out to evaluate the phenolic composition, antioxidant capacities, sugars and organic acids content of sweet cherry cultivars (Bianca, Burlat, Johanna 1, Johanna 2, Kordia, Kunzes Kirsche, Merton Premier, Napoleon, Orleans, Regina, Rivan, Schneiders Spate Knorpelkirsche, Summit, Trebnitzer Lotkirsche grown in Poland. Significant differences were observed between tested cultivars for all studied parameters.

  5. Effect of cultivar and variety on phenolic compounds and antioxidant activity of cherry wine.

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    Xiao, Zuobing; Fang, Lingling; Niu, Yunwei; Yu, Haiyan

    2015-11-01

    To compare the influence of cultivar and variety on the phenolic compounds and antioxidant activity (AA) of cherry wines, total phenolic (TP), total flavonoid (TF), total anthocyanin (TA), total tannin (TT), five individual phenolic acids, and AA were determined. An ultra-performance liquid chromatography tandem mass spectrometry (HPLC-DAD/ESI-MS) method was developed for the determination of gallic acid (GAE), p-hydroxybenzoic acid (PHB), chlorogenic acid (CHL), vanillic acid (VAN), and caffeic acid (CAF). A principal component analysis (PCA) and a cluster analysis (CA) were used to analyze differences related to cultivar and variety. The TP, TF, TA, TT, and AA of samples sourced from the Shandong province of China were higher than those from the Jiangsu province. The PCA and CA results showed that phenolic compounds in cherry wines were closely related to cultivar and variety and that cultivar had more influence on the phenolic compounds of cherry wines than variety.

  6. In vitro bioavailability of phenolic compounds from five cultivars of frozen sweet cherries (Prunus avium L.).

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    Fazzari, Marco; Fukumoto, Lana; Mazza, Giuseppe; Livrea, Maria A; Tesoriere, Luisa; Marco, Luigi Di

    2008-05-28

    The bioavailability of phenolic compounds from five cultivars of frozen sweet cherries was assessed by a digestion process involving pepsin-HCl digestion (to simulate gastric digestion) and pancreatin digestion with bile salts (to simulate small intestine conditions) and dialyzed to assess serum- and colon-available fractions. After pepsin digestion, the % recovery of total phenolics, relative to the original starting material, increased, whereas the % anthocyanins did not change. Following pancreatic digestion and dialysis, the total phenolics in the IN (serum-available) fraction was about 26-30% and the OUT (colon-available) fraction was about 77-101%. The anthocyanin content in the IN fraction was 15-21%, and in the OUT fraction, it was 52-67%. Skeena, Lapins, and Sweetheart cultivars contained higher levels of total phenolics and anthocyanins, which resulted in higher concentrations of these compounds in the IN and OUT fractions. The potential bioavailability of phenolic compounds was also assessed in Bing and Lapins cultivars at three ripening stages. Immature cherries had higher % total phenolics in the IN fraction than mature or overmature cherries. However, immature cherries had the lowest concentrations of these compounds, making the actual bioavailable amounts of these compounds lower than for mature and overmature fruit. High-performance liquid chromatography analysis of Lapins cherries at three maturity stages confirmed the results obtained using spectrophotometric methods for total phenolics and anthocyanins.

  7. Physicochemical characteristics, antioxidant activity, organic acid and sugar contents of 12 sweet cherry (Prunus avium L.) cultivars grown in Turkey.

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    Hayaloglu, Ali Adnan; Demir, Nurullah

    2015-03-01

    Physical characteristics, antioxidant activity and chemical constituents of 12 cultivars (Prunus avium L.) of sweet cherry (Belge, Bing, Dalbasti, Durona di Cesena, Lambert, Merton Late, Starks Gold, Summit, Sweetheart, Van, Vista, and 0-900 Ziraat) were investigated. Significant differences (P cherries. Four different sugars were observed in the samples and their concentrations ordered as glucose > fructose > sucrose > xylose. Sugar alcohol in the cherries was represented by sorbitol (more than 90%) and its concentration varied between 13.93 and 27.12 g/kg. As a result significant differences were observed among the physical properties and chemical constituents of the cherry cultivars.

  8. GENETIC DIVERSITY OF SOME IRANIAN SWEET CHERRY (PRUNUS AVIUM) CULTIVARS USING MICROSATELLITE MARKERS AND MORPHOLOGICAL TRAITS.

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    Farsad, A; Esna-Ashari, M

    2016-01-01

    The aim of this study was to characterize 23 important Iranian sweet cherry (Prunus avium) cultivars collected from different provinces of Iran and 1 foreign cultivar, which was used as control, considered for breeding programs by using 21 microsatellite markers and 27 morphological traits. In sweet cherry (Prunus avium) accessions, leaf, fruit, and stone morphological characters were evaluated during two consecutive years. The study revealed a high variability in the set of evaluated sweet cherry accessions. The majority of important correlations were determined among variables representing fruit and leaf size and variables related to color. Cluster analysis distinguished sweet cherry accessions into two distinct groups. Principal component analysis (PCA) of qualitative and quantitative morphological parameters explained over 86.59% of total variability in the first seven axes. In PCA, leaf traits such as leaf length and width, and fruit traits such as length, width, and weight, and fruit flesh and juice color were predominant in the first two components, indicating that they were useful for the assessment of sweet cherry germplasm characterization. Out of 21 SSR markers, 16 were polymorphic, producing 177 alleles that varied from 4 to 16 alleles (9.35 on average) with a mean heterozygosity value of 0.82 that produced successful amplifications and revealed DNA polymorphisms. Allele size varied from 95 to 290 bp. Cluster analyses showed that the studied sweet cherry genotypes were classified intofive main groups based mainly on their species characteristics and SSR data. In general, our results did not show a clear structuring of genetic variability within the Iranian diffusion area of sweet cherry, so it was not possible to draw any indications on regions of provenance delimitation. The results of this study contribute to a better understanding of sweet cherry genetic variations in Iran, thus making for more efficient programs aimed at preserving biodiversity and

  9. Pomological properties of sweet cherry cultivars grafted on ‘colt’ rootstock

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    Milatović Dragan P.

    2013-01-01

    Full Text Available Pomological properties of 11 newly sweet cherry cultivars grafted on Colt rootstock were studied in the region of Macva over a three-year period (2010- 2012. An investigation has included phenological traits (flowering and maturing, the elements of productivity (number of flowers in an inflorescence, fruit set and yield, the most important fruit characteristics (physical, chemical and sensory, and fruit cracking index. Average time of flowering of tested cultivars was in the first and the second decade of April, while the maturing time was between 15 May (‘Early Lory’ and 20 June (‘Penny’. The highest yield was achieved by cultivars ‘Giorgia’, ‘Sunburst’, and ‘Crystalina’. Large fruit size was found in cultivars ‘Penny’, ‘Santina’, ‘Sunburst’, ‘Olympus’, and ‘Kordia’. The lowest susceptibility to fruit cracking was manifested in cultivars ‘Early Lory’ and ‘Giorgia’. Cultivars ‘Kordia’ and ‘Cristalina’ were given the highest sensory ratings. Overall, the best results were shown by medium-early season cultivar ‘Santina’, which can be recommended for commercial cultivation. In addition, the early-season cultivar ‘Early Lory’, and the late-season cultivar ‘Kordia’ can also be recommended for cultivation. [Projekat Ministarstva nauke Republike Srbije, br. TR 31063

  10. EFFECTS OF DWARFING GISELA 5 ROOTSTOCK ON REPRODUCTIVE POTENTIAL, VEGETATIVE GROWTH, AND PHYSIOLOGICAL FEATURES OF SOME SWEET CHERRY CULTIVARS IN HIGH-DENSITY SWEET CHERRY ORCHARDS

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    Monica Popescu

    2015-12-01

    Full Text Available Gisela 5 rootstock is most important in terms of reducing the vigor of growth. The varieties grafted on Gisela 5 had good horticultural results in terms of yield, adaptability and dwarf growth. This study was aimed to evaluate the growth and physiological behavior of the most popular sweet cherry cultivars in Europe grafted of Gisela 5 rootstock in one of the most important fruit growing area from Romania. The rootstock – scion combinations namely Skeena, Kordia and Ferrovia were grafted on Gisela 5 dwarf rootstocks. Gisela 5 influenced significantly the trunk cross section area among all the tested cultivars (p<0.05. Ferrovia cultivar was the most vigorous in terms of trunk cross sectional area and total annual growth length. Total annual growth was lower for Kordia (1225.61 cm. The ratio between Chl a and Chl b seems to be constant in all grafted plants. The photosynthesis rate [µ mol (CO2 m-2 s-1] varied from 24.12 µ mol (CO2 m-2 s-1 in the Kordia grafted sweet cherry variety to 25.80 µ mol (CO2 m-2 s-1 in the Ferrovia sweet cherry cultivar. Data obtained from field measurements and laboratory observations demonstrated that the Gisela 5 rootstock is compatible with foreign sweet cherry varieties under the selected growing area and can be used to achieve high-density sweet cherry orchards.

  11. Flowering time and incompatibility groups: Cultivar combination in commercial sweet cherry (Prunus avium L. orchards

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    Radičević Sanja

    2011-01-01

    Full Text Available The paper presents results of eight-year study (1999-2006 of flowering phenophase in 21 introduced sweet cherry cultivars grown under the agro-environmental conditions of West Serbia. Flowering time, as well as progress and abundance of flowering were studied, and classification of the studied cultivars according to flowering time was derived. On the basis of mean several-year overlap in phenophase of full flowering and on the grounds of so far known data on classification of these cultivars among incompatibility groups, we have offered a recommendation for their cultivation in orchards whereby the most effective pollination and fertilization can be ensured as well as good fruit-set and satisfactory fruit yields.

  12. Genetic diversity and relatedness of sweet cherry (prunus avium L.) cultivars based on single nucleotide polymorphic markers.

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    Fernandez I Marti, Angel; Athanson, Blessing; Koepke, Tyson; Font I Forcada, Carolina; Dhingra, Amit; Oraguzie, Nnadozie

    2012-01-01

    Most previous studies on genetic fingerprinting and cultivar relatedness in sweet cherry were based on isoenzyme, RAPD, and simple sequence repeat (SSR) markers. This study was carried out to assess the utility of single nucleotide polymorphism (SNP) markers generated from 3' untranslated regions (UTR) for genetic fingerprinting in sweet cherry. A total of 114 sweet cherry germplasm representing advanced selections, commercial cultivars, and old cultivars imported from different parts of the world were screened with seven SSR markers developed from other Prunus species and with 40 SNPs obtained from 3' UTR sequences of Rainier and Bing sweet cherry cultivars. Both types of marker study had 99 accessions in common. The SSR data was used to validate the SNP results. Results showed that the average number of alleles per locus, mean observed heterozygosity, expected heterozygosity, and polymorphic information content values were higher in SSRs than in SNPs although both set of markers were similar in their grouping of the sweet cherry accessions as shown in the dendrogram. SNPs were able to distinguish sport mutants from their wild type germplasm. For example, "Stella" was separated from "Compact Stella." This demonstrates the greater power of SNPs for discriminating mutants from their original parents than SSRs. In addition, SNP markers confirmed parentage and also determined relationships of the accessions in a manner consistent with their pedigree relationships. We would recommend the use of 3' UTR SNPs for genetic fingerprinting, parentage verification, gene mapping, and study of genetic diversity in sweet cherry.

  13. Genetic diversity and relatedness of sweet cherry (Prunus avium L. cultivars based on single nucleotide polymorphic (SNP markers

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    Angel eFernandez i Marti

    2012-06-01

    Full Text Available Most previous studies on genetic fingerprinting and cultivar relatedness in sweet cherry were based on isoenzyme, RAPD and SSR markers. This study was carried out to assess the utility of SNP markers generated from 3’UTRs for genetic fingerprinting in sweet cherry. A total of 114 sweet cherry germplasm representing advanced selections, commercial cultivars and old cultivars imported from different parts of the world were screened with 7 SSR markers developed from other Prunus species and with 40 SNPs obtained from 3’UTR sequences of Rainier and Bing sweet cherry cultivars. Both types of marker study had 99 accessions in common. The SSR data was used to validate the SNP results. Results showed that the average number of alleles per locus, mean observed heterozygosity, expected heterozygosity and polymorphic information content (PIC values were higher in SSRs than in SNPs although both set of markers were similar in their grouping of the sweet cherry accessions as shown in the dendrogram. SNPs were able to distinguish sport mutants from their wild type germplasm. For example, ‘Stella’ was separated from ‘Compact Stella’. This demonstrates the greater power of SNPs for discriminating mutants from their original parents than SSRs. In addition, SNP markers confirmed parentage and also determined relationships of the accessions in a manner consistent with their pedigree relationships. We would recommend the use of 3’ UTR SNPs for genetic fingerprinting, parentage verification, gene mapping and study of genetic diversity in sweet cherry.

  14. Genetic Diversity of Some Sweet Cherry Cultivars Based on Molecular Markers

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    Ioana Virginia Berindean

    2016-11-01

    Full Text Available Sweet cherry (Prunus avium L., originated around the Caspian and Black Sea, is an important fruit tree species of economic interest, and hence, breeding and conservation are requested (. Genetic analysis at the molecular level can be used effectively to study molecular polymorphism existing between intraspecific and interspecific tree species and phylogenetic relationships between them and their hybrids. The purpose of this study was to characterize and determine genetic relationships among the sweet cherry native genotypes belonging to Fruit Research & Development Station Bistrita, Romania, using RAPD markers. To eliminate the existence of possible synonyms from national romanian collection, we collect four Van cultivars, from four different national collection. For molecular analysis of the 16 varieties of sweet cherry were considered 13 RAPD primers selected from the literature. They were later used to determine the genetic variability at the molecular level using PAST program, and the dendrogram was generated based on Jaccard’s genetic distance. The dendrogram constructed by PAST software. The quantity and quality of the DNA obtained was suitable to achieve PCR amplification step. Only seven out of the 13 RAPD primers have generate polymorphic bands. The rest of seven were monomorphics. The most polymorphic primer was OPB10 which generated 11 bands from which 100% were polymorphic.Seven RAPD primers generated a high level of polymorphism which allowed to divide these cherry varieties into two groups according to their genetic geographical origin and the pedigree.

  15. The importance of bee pollination of the sour cherry (Prunus cerasus Cultivar ‘Stevnsbaer’ in Denmark

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    Lise Hansted

    2012-12-01

    Full Text Available Low fruit set, despite normally-developed flowers, is often a significant contributor to poor yield of the self-fertile sour cherry (Prunus cerasus cultivar ‘Stevnsbaer’ in Denmark. The aim of this study was to investigate the effect of insect, and particularly, bee pollination on the fruit set of this cultivar, in order to provide orchard management information for both Danish ‘Stevnsbaer’ growers and beekeepers. Visits to cherry flowers by honey bees (Apis mellifera, Bombus species and solitary bees, were recorded during the flowering of ‘Stevnsbaer’ in five separate Danish orchards. The results indicate that there is a significantly higher fruit set on open pollinated branches when compared to caged branches, where bees and other pollinating insects where excluded. The results were qualitatively consistent over three different seasons (2007, 2009 and 2010. A period of prolonged cold, humid weather before and during early flowering probably reduced fruit set significantly in 2010 compared to 2009. Regarding the apparent benefits of bee pollination on fruit set and subsequent implications for yield, we recommend placing honeybees in ‘Stevnsbaer’ orchards during flowering to sustain commercially viable production. Another valuable management strategy would be to improve foraging and nesting conditions to support both honey and wild bees in and around the orchards.

  16. INFLUENCE OF THE ROOT TYPE ON THE FRUIT QUALITY OF THREE SOUR CHERRY CULTIVARS

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    D. SOTIROV

    2005-08-01

    Full Text Available The investigations were conducted in the period 2003-2005 at the Institute of Agriculture in Kyustendil /Bulgaria/ with three sour cherry cultivars - Heimanns rubinveichsel, M-15 and Nefris, self-rooted and grafted on IK-M9 mahaleb rootstock. The yield per tree, the fruit weight and the content of dry matter, the total sugars and total acids of the fruits were evaluated. The fruit yield of the self-rooted trees was smaller than that of the grafted ones in all experimental years, but the statistical analysis showed significant differences only for cv. Nefris. The rootstock used or its absence did not have considerable influence on the fruit weight. Significant differences in the fruit chemical composition between the grafted and self-rooted trees were not found and it depended mainly on the cultivar and to a smaller extent on the rootstock or its absence.

  17. Characterization of Four Popular Sweet Cherry Cultivars Grown in Greece by Volatile Compound and Physicochemical Data Analysis and Sensory Evaluation

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    Maria V. Vavoura

    2015-01-01

    Full Text Available Volatile compounds, physicochemical and sensory attributes of four sweet cherry cultivars (Canada giant, Ferrovia, Lapins and Skeena grown in Northern Greece were determined. Eighteen volatile compounds were identified and semi-quantified in cherries using solid phase micro extraction in combination with gas chromatography/mass spectrometry (SPME-GC/MS. Carbonyl compounds were the most abundant in sweet cherry aroma, followed by alcohols, esters and hydrocarbons/terpenes. Cherry cultivars in order of increasing amounts of volatiles were: Lapins < Canada giant < Ferrovia < Skeena. Physicochemical parameters determined included: titratable acidity (TA, pH, total soluble solids (TSS, maturity index (MI and total phenolic content (TPC. TA ranged between 0.21 and 0.28 g malic acid/100 g fresh weight (FW. The pH ranged between 3.81 and 3.96. TSS ranged between 13.00 and 16.00 °Brix. MI ranged between 51.8 and 75.0. TPC ranged between 95.14 and 170.35 mg gallic acid equivalents (GAE/100 g FW. Sensory evaluation showed that cherry colour, in order of increasing intensity, was: Canada giant < Ferrovia < Lapins < Skeena. Respective order for cherry firmness was: Canada giant < Lapins ≤ Ferrovia < Skeena and for flavour: Lapins < Canada giant < Skeena ≤ Ferrovia. Correlation of volatiles to physicochemical and sensory attributes showed varying trends.

  18. Phenolic Compounds, Volatiles, and Sensory Characteristics of Twelve Sweet Cherry (Prunus avium L.) Cultivars Grown in Turkey.

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    Hayaloglu, Ali Adnan; Demir, Nurullah

    2016-01-01

    The paper reports the phenolic, anthocyanin, and volatile compounds and sensory characteristics of 12 cultivars of sweet cherries including cvs. Belge, Bing, Dalbasti, Durona di Cesena, Lambert, Merton Late, Starks Gold, Summit, Sweetheart, Van, Vista, and 0-900 Ziraat. Eight individual phenolic compounds were determined by the HPLC-DAD method. Among these cherries, cvs. Bing, Durona di Cesena, and Lambert contained higher levels of total individual phenolic compounds than the other cultivars. Six anthocyanins were detected in cherries and cyanidin-3-O-rutinoside was principal and it was the highest level in cv. Bing. The major volatiles found were 1-hexanol, (E)-2-hexen-1-ol, benzylalcohol, hexenal, (E)-2-hexenal, and benzaldehyde. Sensory evaluation of the cherries showed that cvs. Belge, Bing, Dalbasti, and Summit have higher textural and flavor scores than others. It was concluded that the same compounds for phenolic or volatiles profiles of sweet cherries were similar in qualitative; however, quantitative differences were observed in these cultivars.

  19. Genetic Diversity and Relatedness of Sweet Cherry (Prunus Avium L.) Cultivars Based on Single Nucleotide Polymorphic Markers

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    Fernandez i Marti, Angel; Athanson, Blessing; Koepke, Tyson; Font i Forcada, Carolina; Dhingra, Amit; Oraguzie, Nnadozie

    2012-01-01

    Most previous studies on genetic fingerprinting and cultivar relatedness in sweet cherry were based on isoenzyme, RAPD, and simple sequence repeat (SSR) markers. This study was carried out to assess the utility of single nucleotide polymorphism (SNP) markers generated from 3′ untranslated regions (UTR) for genetic fingerprinting in sweet cherry. A total of 114 sweet cherry germplasm representing advanced selections, commercial cultivars, and old cultivars imported from different parts of the world were screened with seven SSR markers developed from other Prunus species and with 40 SNPs obtained from 3′ UTR sequences of Rainier and Bing sweet cherry cultivars. Both types of marker study had 99 accessions in common. The SSR data was used to validate the SNP results. Results showed that the average number of alleles per locus, mean observed heterozygosity, expected heterozygosity, and polymorphic information content values were higher in SSRs than in SNPs although both set of markers were similar in their grouping of the sweet cherry accessions as shown in the dendrogram. SNPs were able to distinguish sport mutants from their wild type germplasm. For example, “Stella” was separated from “Compact Stella.” This demonstrates the greater power of SNPs for discriminating mutants from their original parents than SSRs. In addition, SNP markers confirmed parentage and also determined relationships of the accessions in a manner consistent with their pedigree relationships. We would recommend the use of 3′ UTR SNPs for genetic fingerprinting, parentage verification, gene mapping, and study of genetic diversity in sweet cherry. PMID:22737155

  20. Genetic Characterization and Relatedness among Cherry Cultivars in a Germplasm Bank by Randomly Amplified Polymorphic DNA Analysis

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    Jesus Moreno

    2005-12-01

    Full Text Available Random amplified polymorphic DNA (RAPD analysis was performed on 38 cultivars of cherry (Prunus avium L. grown in the Jerte Valley, Cáceres, Spain. Thirty five selected decamer primers produced 69 reproducible polymorphic amplification products. The degree of polymorphism detected made possible the identification of all the cultivars by combining the RAPD banding patterns of only seven primers: OPK-08, OPQ-14, OPR-09, OPS-19, OPX-02, OPX-15 and OPZ-13. Eleven unique markers allowed identification of nine cultivars while 15 cultivars were identified by unique banding patterns. A similarity matrix derived from the RAPD amplification products generated by all the primers was obtained using the index of similarity of Jaccard. The similarity coefficients among cultivars ranged from 0.27 to 0.81 with an average of 0.50. A dendrogram based on UPGMA clustering method was constructed using the similarity matrix. The dendrogram showed a good correlation between the clustering of cherry cultivars and their geographic origin, especially revealing a stronger genetic proximity between some of the most characteristic cultivars of the Jerte Valley. This result supports the autochthonous origin hypothesis for these cultivars.

  1. Compositional changes in cell wall polysaccharides from five sweet cherry (Prunus avium L.) cultivars during on-tree ripening.

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    Basanta, María F; Ponce, Nora M A; Salum, María L; Raffo, María D; Vicente, Ariel R; Erra-Balsells, Rosa; Stortz, Carlos A

    2014-12-24

    Excessive softening is a major cause of postharvest deterioration during transportation and storage of fresh cherries. In continuing our studies to identify the factors determining the textural differences between sweet cherry fruit genotypes, we evaluated the solubilization, depolymerization, and monosaccharide composition of pectin and hemicelluloses from five sweet cherry cultivars ('Chelan', 'Sumele', 'Brooks', 'Sunburst', and 'Regina') with contrasting firmness and cracking susceptibility at two developmental stages (immature and ripe). In contrast to what is usually shown in most fruits, cherry softening could occur is some cultivars without marked increases in water-soluble pectin. Although polyuronide and hemicellulose depolymerization was observed in the water-soluble and dilute-alkali-soluble fractions, only moderate association occurs between initial polymer size and cultivar firmness. In all the genotypes the Na2CO3-soluble polysaccharides (NSF) represented the most abundant and dynamic wall fraction during ripening. Firm cultivars showed upon ripening a lower neutral sugars/uronic acid ratio in the NSF, suggesting that they have a lower proportion of highly branched polyuronides. The similar molar ratios of arabinose plus galactose to rhamnose [(Ara+Gal)/Rha] suggest that the cultivars differed in their relative proportion of homogalacturonan (HG) and rhamnogalacturonan I (RG-I) rather than in the size of the RG side chains; with greater proportions of HG in firmer cherries. Ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was useful to identify the depolymerization patterns of weakly bound pectins, but gave less accurate results on ionically bound pectins, and was unable to find any pattern on covalently bound pectins.

  2. [Identifying the S genotypes of sweet cherry (Prunus avium L.) cultivars].

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    Chen, Xiao-Liu; Chen, Xue-Sen; Shu, Huai-Rui

    2004-10-01

    This report identified S-RNase genes (S genes) of sweet cherry (Prunus avium L.), presented the sequences of S genes by using a pair of specific primers PruC2 and PruC4R based on the conserved regions C2 and RC4 of Rosaceous S-RNase genes and researched the S gene specific products from the genomic DNAs of different cultivars in which most of the S genotypese were unknown. The bands of PCR were cloned and their sequences were compared in GenBank. Four S genes were defined and the conclusion was made that all the same bands from PCR in the agarose gel had the same length and sequence of nucleic acid and were the same kind of S gene. The lengths of the amplified S genes are as follows: S1 is 677 bp, S3 762 bp, S4 945 bp, S6 456 bp. The S genotypes (S gene genotypes) of the tested self-incompatible cultivars were identified as follows: 'Hongdeng', 'Hongyan' and 'Early ruby', as same as 'Van', were S1 S3; 'Jueze', 'Hongfeng' and 'Napoleon' were S3S4; 'Dazi' was S1 S6; 'Changbahong' was S1 S4; 'Elton' was S3S6. The self-compatibile cultivars 'Waiyin No.7' and 'Stella' had the same S genotypes S3 S4'.

  3. The effect of temperature on pollen germination and pollen tube growth of sour cherry cultivars

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    Milatović Dragan P.

    2014-01-01

    Full Text Available The study was carried out to determine the effect of three different temperatures (5, 15 and 25°C on in vitro pollen germination and pollen tube growth of five sour cherry cultivars: ‘Heimanns Konservenweichsel’, ‘Kelleriis 14’, ‘Oblačinska’, ‘Rexelle’ and ‘Šumadinka’. Pollen germination a % agar % Temperature significantly affected pollen germination. High germination rates (50-70% were obtained at both 15°C and 25°C. Satisfactory germination rates (42-51% were also obtained at 5°C in some cultivars (‘Rexelle’, ‘Šumadinka’ and ‘Heimanns Konservenweichsel’. The influence of temperature on the pollen tube growth was more prominent. The length of pollen tubes was three to six times higher at 15°C and 25°C in comparison with 5°C. This has led to the conclusion that the temperature of 5°C, although it could be adequate for pollen germination, is not high enough for optimal pollen tube growth. was determined by germinating pollen grains in culture medium containing 0.7agar-and 15sucrose. [Projekat Ministarstva nauke Republike Srbije, br. TR-31063

  4. Quality of sour cherry juice of different clones and cultivars (Prunus cerasus L.) determined by a combined sensory and NMR spectroscopic approach

    DEFF Research Database (Denmark)

    Clausen, Morten Rahr; Pedersen, Bjarne Hjelmsted; Bertram, Hanne Christine S.;

    2011-01-01

    Juice was manufactured from seven different sour cherry clones/cultivars and evaluated by quantitative descriptive sensory analysis and 1H NMR spectroscopy. The sensory evaluation showed a large variation in several sensory attributes between the sour cherry clones/cultivars, which could be divided...... quality of different sour cherry clones. In addition, malic acid was the most important metabolite for modeling the two highly correlated sensory attributes sweetness and sourness, whereas the glucose content had a slight effect and the fructose content had no impact on sweetness/sourness. Other...

  5. Eating quality and health-promoting properties of two sweet cherry (Prunus avium L.) cultivars stored in passive modified atmosphere.

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    Lara, Isabel; Camats, Josep A; Comabella, Eva; Ortiz, Abel

    2015-03-01

    Two sweet cherry cultivars ('New Star' and 'Sweet Heart') were chosen to explore the impact of passive modified atmosphere packaging on the eating quality and health-promoting properties of fruit. Packaged and unpackaged fruits were stored at 0 ℃ for 15 or 30 days, followed by zero or three days at 20 ℃, after which the analyses were undertaken. In most cases, modified atmosphere packaging helped preserving higher firmness values and reducing the incidence of alterations and decay in both cultivars, but the effects on other physicochemical attributes were different for each cultivar. Partial least squares regression procedures were used to reveal relationships among the different variables assessed. Generally, fruits displaying higher antioxidant capacity were also characterised by higher values for firmness and titratable acidity, in turn related to better acceptability scores in both cultivars. However, the attributes contributing most to acceptability were different in each case. In 'New Star' fruit, acceptability was closely related to the perception of cherry flavour. In this cultivar, acetaldehyde content was related to the perception of off-flavours, while ethanol content was found to associate to soluble solids and to the perception of sweetness. In contrast, acceptability of 'Sweet Heart' fruit was related mainly to the perception of firmness and, to a lower extent, of sweetness.

  6. Preharvest application of oxalic acid increased fruit size, bioactive compounds, and antioxidant capacity in sweet cherry cultivars (Prunus avium L.).

    Science.gov (United States)

    Martínez-Esplá, Alejandra; Zapata, Pedro Javier; Valero, Daniel; García-Viguera, Cristina; Castillo, Salvador; Serrano, María

    2014-04-16

    Trees of 'Sweet Heart' and 'Sweet Late' sweet cherry cultivars (Prunus avium L.) were treated with oxalic acid (OA) at 0.5, 1.0, and 2.0 mM at 98, 112, and 126 days after full blossom. Results showed that all treatments increased fruit size at harvest, manifested by higher fruit volume and weight in cherries from treated trees than from controls, the higher effect being found with 2.0 mM OA (18 and 30% higher weight for 'Sweet Heart' and 'Sweet Late', respectively). Other quality parameters, such as color and firmness, were also increased by OA treatments, although no significant differences were found in total soluble solids or total acidity, showing that OA treatments did not affect the on-tree ripening process of sweet cherry. However, the increases in total anthocyanins, total phenolics, and antioxidant activity associated with the ripening process were higher in treated than in control cherries, leading to fruit with high bioactive compounds and antioxidant potential at commercial harvest (≅45% more anthocyanins and ≅20% more total phenolics). In addition, individual anthocyanins, flavonols, and chlorogenic acid derivatives were also increased by OA treatment. Thus, OA preharvest treatments could be an efficient and natural way to increase the quality and functional properties of sweet cherries.

  7. In vitro plant regeneration from leaves and internode sections of sweet cherry cultivars (Prunus avium L.).

    Science.gov (United States)

    Matt, Andrea; Jehle, Johannes A

    2005-10-01

    Regeneration of adventitious shoots from leaves and, for the first time, from internode sections were compared and optimized for five economically important sweet cherry cultivars, i.e. "Schneiders", "Sweetheart", "Starking Hardy Giant", "Kordia" and "Regina" (Prunus avium L.). The influence of basal media, carbon source, combination and dosage of phytohormones, ethylene inhibitor such as silver thiosulfate and a 16 h:8 h light:dark photoperiod versus complete darkness were evaluated. Both, DKW/WPM (1:1) and Quoirin/Lepoivre (QL) basal media stimulated organogenesis more than QL/WPM (1:1), Chee and Pool (CP), Murashige Skoog (MS), Driver and Kuniyuki (DKW) or woody plant (WPM) media did. An induction phase in darkness resulted in lower or zero regeneration rates. The best regeneration efficiencies were generally obtained with thidiazuron in combination with indole-3-butyric-acid. The addition of silver thiosulfate resulted in a similar or reduced regeneration efficiency. Significant genotypic variability in adventitious bud formation was evident for both explant sources, leaf and internode section. Adventitious shoots were obtained from 11% of leaf explants and 50% of internode sections indicating that shoot regeneration from internodes was significantly more efficient than from leaves.

  8. Variability components and heritability of pomological and chemical characteristics in sour cherry clones of cultivar Montmorency

    Directory of Open Access Journals (Sweden)

    Fotirić Milica

    2007-01-01

    Full Text Available In 12 sour cherry clones of cultivar Montmorency 5 pomological (fruit length, fruit width, fruit weight, stone weight and fruit stem length and 4 chemical characteristics (soluble solid content, total acid content, invert sugar content and total sugar content were investigated. Based on results of analysis of variance for all examined characteristics, variability components, coefficients of genetic and phenotypic variation and coefficient of heritability in a broader sense were calculated. Considering components of total variability, year represented the component that caused variability of majority examined characteristics in the highest percentage. Coefficients of genetic variation showed that in investigated clones total acid content varied the least (CVg = 1.79%, and the most fruit weight (CVg = 11.41%. The lowest phenotypic varying was determined for fruit length (CVf = 4.01% but the highest for fruit weight (CVf = 13.86%. Coefficient of heritability was the highest for stone weight (h2 = 70.27%, and the lowest for total acid content (h2 = 7.73%.

  9. Post-storage cell wall metabolism in two sweet cherry (Prunus avium L.) cultivars displaying different postharvest performance.

    Science.gov (United States)

    Belge, Burcu; Comabella, Eva; Graell, Jordi; Lara, Isabel

    2015-09-01

    The biochemical processes underlying firmness loss of sweet cherry (Prunus avium L.) fruit are poorly understood. Studies on cell wall metabolism of sweet cherry have been generally undertaken during on-tree development or at harvest maturity, while published reports on postharvest changes are scarce and fragmentary. In this work, cell wall modifications after storage at 0 ℃ were studied in two cherry cultivars ('Celeste' and 'Somerset') displaying different postharvest potential. Firmness was largely determined by the yields of the Na2CO3- and KOH-soluble fractions, enriched in covalently-bound pectins and in matrix glycans, respectively, and correlated well with ascorbic acid contents. The yields of these two cell wall fractions were correlated inversely with pectinmethylesterase and endo-1,4-β-d-glucanase activities, indicating a relevant role of these two enzymes in postharvest firmness changes in sweet cherry. The amount of solubilised cell wall materials was closely associated to the contents of dehydroascorbic acid, suggesting the possible involvement of oxidative mechanisms in cell wall disassembly. These data may help understanding the evolution of fruit quality during the marketing period, and give hints for the design of suitable management strategies to preserve key attributes.

  10. Soluble Sugars and Sucrose-Metabolizing Enzymes Related to Cold Acclimation of Sweet Cherry Cultivars Grafted on Different Rootstocks

    Directory of Open Access Journals (Sweden)

    Ece Turhan

    2012-01-01

    Full Text Available The bark tissues were collected from 4-year-old sweet cherry trees cvs. 0900 Ziraat and Lambert grafted on Gisela 5 and Mazzard rootstocks in cold-acclimated (CA and nonacclimated (NA stages. Bark tissues subjected to 4°C and −5°C injured to a limited extent in both stages. However, more than 50% injury occurred by temperatures equal to or colder than −15°C only in NA period. Total soluble sugar (TSS, reducing sugars, and sucrose contents were higher in CA than those in NA stages in all samples. The activities of acid invertase (EC 3.2.1.26 and sucrose synthase (SS (EC 2.4.2.13 enzymes were higher in NA stage than those in CA stage. Considering the rootstocks, reducing sugars were higher in both cultivars grafted on Gisela 5 whereas sucrose contents were higher in both cultivars grafted on Mazzard. However, the enzyme activities of both cultivars were higher on Mazzard rootstock than on Gisela 5. In conclusion, cold hardiness of sweet cherry graft combinations was suggested by increasing their TSS, reducing sugars, and sucrose contents significantly in the CA stage. Moreover, acid invertase and SS are down regulated during cold acclimation. Indeed the results suggested that Mazzard is more cold-hardy rootstock than Gisela 5.

  11. Construction and comparative analyses of highly dense linkage maps of two sweet cherry intra-specific progenies of commercial cultivars.

    Directory of Open Access Journals (Sweden)

    Carolina Klagges

    Full Text Available Despite the agronomical importance and high synteny with other Prunus species, breeding improvements for cherry have been slow compared to other temperate fruits, such as apple or peach. However, the recent release of the peach genome v1.0 by the International Peach Genome Initiative and the sequencing of cherry accessions to identify Single Nucleotide Polymorphisms (SNPs provide an excellent basis for the advancement of cherry genetic and genomic studies. The availability of dense genetic linkage maps in phenotyped segregating progenies would be a valuable tool for breeders and geneticists. Using two sweet cherry (Prunus avium L. intra-specific progenies derived from crosses between 'Black Tartarian' × 'Kordia' (BT×K and 'Regina' × 'Lapins'(R×L, high-density genetic maps of the four parental lines and the two segregating populations were constructed. For BT×K and R×L, 89 and 121 F(1 plants were used for linkage mapping, respectively. A total of 5,696 SNP markers were tested in each progeny. As a result of these analyses, 723 and 687 markers were mapped into eight linkage groups (LGs in BT×K and R×L, respectively. The resulting maps spanned 752.9 and 639.9 cM with an average distance of 1.1 and 0.9 cM between adjacent markers in BT×K and R×L, respectively. The maps displayed high synteny and co-linearity between each other, with the Prunus bin map, and with the peach genome v1.0 for all eight LGs (LG1-LG8. These maps provide a useful tool for investigating traits of interest in sweet cherry and represent a qualitative advance in the understanding of the cherry genome and its synteny with other members of the Rosaceae family.

  12. Prunus avium: nuclear DNA study in wild populations and sweet cherry cultivars.

    Science.gov (United States)

    Guarino, Carmine; Santoro, Simona; De Simone, Luciana; Cipriani, Guido

    2009-04-01

    The PCR-SSR technique was used to detect nuclear DNA diversity in five wild populations of Prunus avium from deciduous forests in Italy, Slovenia, and Croatia and 87 sweet cherry accessions from different geographical areas that have been maintained in the sweet cherry collection in Italy. This sweet cherry collection includes local accessions from the Campania Region as well as accessions from different countries. Twenty-eight microsatellites, previously developed in this species, generated polymorphic amplification products. Between 2 and 14 alleles were revealed for the polymorphic loci studied, with the expected heterozygosity ranging from 0.045 to 0.831. The total probability of identity was 56.94 x 10-18. A model-based Bayesian clustering analysis identified nine distinct gene pools in cultivated P. avium. The probability that wild populations were assigned to cultivated gene pools indicated that three gene pools accounted for the genomic origin of 53% of P. avium sampled. A dendrogram was generated using UPGMA (unweighted pair group method with arithmetic averages) based on Nei genetic distance analysis. This dendrogram classified most of the genotypes into one major group with an additional group of five accessions. The results indicate that this set of SSRs is highly informative, and they are discussed in terms of the implications for sweet cherry characterization.

  13. Direct photothermal techniques for quantification of anthocyanins in sour cherry cultivars

    NARCIS (Netherlands)

    Doka, O.; Ficzek, G.; Bicanic, D.D.; Spruijt, R.B.; Luterotti, S.; Toth, M.; Buijnsters, J.G.; György Végvári, G.

    2011-01-01

    The analytical performance of the newly proposed laser-based photoacoustic spectroscopy (PAS) and of optothermal window (OW) method for quantification of total anthocyanin concentration (TAC) in five sour cherry varieties is compared to that of the spectrophotometry (SP). High performance liquid chr

  14. Fruit quality and bioactive compounds relevant to human health of sweet cherry (Prunus avium L.) cultivars grown in Italy.

    Science.gov (United States)

    Ballistreri, Gabriele; Continella, Alberto; Gentile, Alessandra; Amenta, Margherita; Fabroni, Simona; Rapisarda, Paolo

    2013-10-15

    The fruit quality characteristics, phenolic compounds and antioxidant capacities of 24 sweet cherry (Prunus avium L.) cultivars grown on the mountainsides of the Etna volcano (Sicily, Italy) were evaluated. High-performance liquid chromatographic methods were used to identify and quantify sugars, organic acids and phenolics. A total of seven phenolic compounds were characterised as hydroxycinnamic acid derivatives (neochlorogenic acid, p-coumaroylquinic acid and chlorogenic acid) and anthocyanins (cyanidin 3-glucoside, cyanidin 3-rutinoside, pelargonidin 3-rutinoside and peonidin 3-rutinoside). The total anthocyanin content ranged from 6.21 to 94.20mg cyanidin 3-glucoside equivalents/100g fresh weight (FW), while the total phenol content ranged from 84.96 to 162.21mg gallic acid equivalents/100g FW. The oxygen radical absorbance capacity (ORAC) assay indicated that fruit of all genotypes possessed considerable antioxidant activity. The high level of phenolic compounds and antioxidant capacity of some sweet cherry fruits implied that they might be sources of bioactive compounds that are relevant to human health.

  15. Influence of Mahaleb and Gisela 5 Rootstocks on the Growth of „Bigarreau Burlat” Sweet Cherry Cultivar

    Directory of Open Access Journals (Sweden)

    Monica Pal

    2016-11-01

    Full Text Available Abstract. The influence of Prunus Mahaleb L. and ‘Gisela 5’ rootstocks on the growth of ‘Biggareau Burlat’ sweet cherry cultivar was evaluate on the environmental conditions of Cluj-Napoca city, in 2015, in a high-density plot (trees are planted at the distance of 4 x 1.5 m with 1666 trees/ha, trained as spindle busch, with trellis system and drip fert-irigation provided. The measurements were done in April, on 10 trees of the cultivar grafted on different rootstock, in the 4th year after planting. The trunck diameter growth was measured 5 cm above the graft, and it was also recorded the number and length of annual increases (long, medium and spur fruiting branches and calculated the tree height. After first four years from planting, ‘Biggareau Burlat’ grafted on ‘Gisela 5’ rootstock proved to be more vigorously than grafted on Prunus Mahaleb L., considering the total numbers of the medium and long branches per tree. ‘Biggareau Burlat’/Gisela 5, compared to ‘Biggareau Burlat’/P. Mahaleb significantly exceeded in the number of medium branches (4.7 comparatively to 3, number of long branches on the tree (17.2 comparatively to 7.9, number of inflorescences buds (74.7 comparatively to 41.3  and the total length of annual tree branches.

  16. Impact of Cultivar on Profile and Concentration of Lipophilic Bioactive Compounds in Kernel Oils Recovered from Sweet Cherry (Prunus avium L.) by-Products.

    Science.gov (United States)

    Górnaś, Paweł; Rudzińska, Magdalena; Raczyk, Marianna; Mišina, Inga; Segliņa, Dalija

    2016-06-01

    Lipophilic bioactive compounds in oils recovered from the kernels of seven sweet cherry (Prunus avium L.) cultivars, harvested at single location in 2013, were studied. Oil yield in sweet cherry ranged between 30.3-40.3 % (w/w) dw. The main fatty acids were oleic acid (39.62-49.92 %), linoleic acid (31.13-38.81 %), α-eleostearic acid (7.23-10.73 %) and palmitic acid (5.59-7.10 %), all four represented approximately 95 % of the total detected fatty acids. The ranges of total tocochromanols and sterols were between 83.1-111.1 and 233.6-419.4 mg/100 g of oil, respectively. Regardless of the cultivar, the γ-tocopherol and β-sitosterol were the main lipophilic minor bioactive compounds. The content of the carotenoids and squalene were between 0.38-0.62 and 60.9-127.7 mg/100 g of oil, respectively. Three significant correlations were found between oil yield and total contents of sterols (r = -0.852), tocochromanols (r = -0.880) and carotenoids (r = -0.698) in sweet cherry kernel oils. The oil yield, as well as the content of lipophilic bioactive compounds in oil was significantly affected by the cultivar.

  17. Tart cherry supplementation improves working memory, hippocampal inflammation, and autophagy in aged rats.

    Science.gov (United States)

    Thangthaeng, Nopporn; Poulose, Shibu M; Gomes, Stacey M; Miller, Marshall G; Bielinski, Donna F; Shukitt-Hale, Barbara

    2016-12-01

    High consumption of fruits and vegetables has been associated with reduced risk of debilitating diseases and improved cognition in aged populations. These beneficial effects have been attributed to the phytochemicals found in fruits and vegetables, which have previously been shown to be anti-inflammatory and modulate autophagy. Tart cherries contain a variety of potentially beneficial phytochemicals; however, little research has been done to investigate the effects of tart cherry on the aging brain. Therefore, the purpose of this study was to determine if tart cherry supplementation can improve cognitive and motor function of aged rats via modulation of inflammation and autophagy in the brain. Thirty 19-month-old male Fischer 344 rats were weight-matched and assigned to receive either a control diet or a diet supplemented with 2 % Montmorency tart cherry. After 6 weeks on the diet, rats were given a battery of behavioral tests to assess for strength, stamina, balance, and coordination, as well as learning and working memory. Although no significant effects were observed on tests of motor performance, tart cherry improved working memory of aged rats. Following behavioral testing, the hippocampus was collected for western/densitometric analysis of inflammatory (GFAP, NOX-2, and COX-2) and autophagy (phosphorylated mTOR, Beclin 1, and p62/SQSTM) markers. Tart cherry supplementation significantly reduced inflammatory markers and improved autophagy function. Daily consumption of tart cherry reduced age-associated inflammation and promoted protein/cellular homeostasis in the hippocampus, along with improvements in working memory. Therefore, addition of tart cherry to the diet may promote healthy aging and/or delay the onset of neurodegenerative diseases.

  18. Characterization of Pseudomonas syringae pathovars from different sweet cherry cultivars by RAPD analysis

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    Iličić Renata

    2016-01-01

    Full Text Available Pseudomonas syringae pvs., isolated from sweet cherry grown on different localities in Serbia, were genetically characterized using RAPD analysis. Four out of eleven tested primers (SPH1, DJP 17, DJ 15, and DJ 16 were selected on the basis of the differences between isolates within two pathovars - syringae and morsprunorum race 1. Cumulative RAPD analysis indicated heterogeneity within the population of both groups of tested isolates, revealing four different patterns in each group. RAPD analysis showed up to 24% differences among pv. syringae isolates, as well as 41% in comparison with the reference strain KFB0103 (pv. syringae, while differences of 15% among isolates pv. morsprunorum 1 race and 36% compared to the reference strain CFBP2119 (pv. morsprunorum 1 were observed. Isolates from locality Selenca exhibited three different genotypic patterns of pv. morsprunorum race 1 and one pattern of pv. syringae. Isolates of pv. morsprunorum collected in the same year from two plant organs (branches and leaves of the cv. Vanda yielded two different patterns. The pv. morsprunorum on cv. Kordia and pv. syringae on cv. Regina were detected at Mikicevo locality. The same patterns were observed for isolates of pv. syringae from Kanjiza and Selenca, as well as from Gornji Tavankut in two years of isolation. Differences were noted between isolates from the same pathovar originating from Ljutovo and Mikicevo, as well as with respect to all other isolates of same pathovar. [Projekat Ministarstva nauke Republike Srbije, br. III46007

  19. Effects of Industrial Processes on Antioxidant Power and Polyphenols Profile in Cherry Tomato Cultivar.

    Science.gov (United States)

    Tommonaro, Giuseppina; De Prisco, Rocco; Pergamo, Rita; Iodice, Carmine; Abbamondi, Gennaro Roberto; Spagnuolo, Annalisa; Nicolaus, Barbara

    2015-10-01

    The antioxidant capacity and the polyphenolic profile of fresh and processed cherry tomatoes were analyzed with the aim of investigating the effect of industrial processes on the nutritional qualities of fruits. The results exhibited a decrease of antioxidant activity mainly in the lipophilic fraction of processed tomatoes compared with fresh products. No great difference in the antioxidant capacity was detected in the hydrophilic and methanolic extracts of fresh tomatoes and processed tomato juices. Moreover, a decrease of polyphenolic content, estimated by means of high-performance liquid chromatography analysis and Folin-Ciocalteu method, was observed in all tomato juices. The overall polyphenolic profile of both fresh and processed tomatoes did not change significantly and, among all juices, a higher polyphenolic content was detected in juice containing peels and seeds in comparison to those without. From our data on antioxidant power and the bioactive metabolite content, tomato juice could be used as a valid and easily available source of antioxidants in everyday diet to preserve human health.

  20. Transcriptional analysis of cell wall and cuticle related genes during fruit development of two sweet cherry cultivars with contrasting levels of cracking tolerance

    Directory of Open Access Journals (Sweden)

    Cristián Balbontín

    2014-04-01

    Full Text Available Rain-induced cracking before harvest is the major cause of crop loss in sweet cherry (Prunus avium [L.] L. In order to better understand the relationship between cherry fruit cracking and gene expression, the transcriptional patterns of six genes related to cell wall modification and cuticular wax biosynthesis were analyzed during fruit setting (FS, fruit color change (FC and fruit ripening (FR, employing two contrasting cultivars: the cracking resistant 'Kordia' and the cracking susceptible 'Bing'. The transcription levels of AP2/EREBP-type transcription factor (PaWINB, wax synthase (WS, ß-ketoacyl-CoA synthase (PaKCS6, and ß-galactosidase (ß-Gal showed higher levels in 'Kordia' than in 'Bing' during the FS stage, while similar values were observed in both cultivars at FR stage. In contrast to that pattern, transcription levels of expansin (PaEXPl were higher at FR stage in 'Kordia' than in 'Bing'. Transcript profile of lipid transport protein gene (PaLTPGl decreased during fruit development, with higher levels in 'Bing' than in 'Kordia' at FC and FR stages suggesting no relation with cracking tolerance. The expression profiles of PaWINB, WS, PaKCS6, and ß-Gal suggest that they are genes involved in conferring cracking tolerance, likely due to their function in cuticle deposition during early stages of fruit development. In addition, a greater expression level of expansin gene would allow for a faster growth rate in 'Kordia' at FR stage.

  1. Parental Selection in Rice Cultivar Improvement

    Institute of Scientific and Technical Information of China (English)

    Stanley Omar P B.SAMONTE; Rodante E.TABIEN; Lloyd T.WILSON

    2013-01-01

    The evaluation of rice (Oryza sativa L.) cultivars assists breeders in identifying useful trait relationships and in selecting parents as donors of specific traits.This study was conducted to compare long-grain rice cultivars using genotype x trait (GT) biplot analysis and determine potential donors of traits related to grain yield and quality.Seventeen cultivars in the 2005 and 2006 Uniform Regional Rice Nursery in Beaumont,Texas,USA were analyzed for 20 traits using GT biplot analysis.The GT biplots showed the diversity among cultivars with regards to yield-related traits.Cultivars recommended as donor parents were:Trenasse,Spring,Presidio,and Cocodrie for high grain yield and head rice rate;Trenasse and Presidio for semi-dwarfness; Banks for tall plant height; Wells for high flag leaf area,panicle weight,and number and mass of filled grains per panicle; Hidalgo for high tiller density; Francis for high number of spikelets per panicle; Spring and Trenasse for short flowering duration; Cheniere for more days to heading and maturity and Spring for less days; and Spring and Hidalgo for high grain weight.Breeders can use these cultivars with specific traits to increase grain yield and quality.

  2. Preliminary evaluation of pollen quality, fertility relations and fruit set of selected sour cherry cultivars in polish conditions

    Directory of Open Access Journals (Sweden)

    Ewa Szpadzik

    2012-12-01

    Full Text Available Preliminary experiments were carried out in spring 2006. The percentage of fruit set of 'Schattenmorelle IR-2', 'Koral', 'Debreceni Bötermö', 'újfefértói Fürtos' and 'Karneol' was higher after open pollination compared with self-pollination. The cultivar Vowi had an inconsiderably higher percentage of fruit set after self-pollination compared with open pollination. The percentage of fruit set in 'Debreceni Bötermö' and 'újfehértói Fürtos' was about 25 % higher after pollination by 'Schattenmorelle IR-2' and 'Koral' compared with the percentage of fruit set after cross - pollination of both cultivars with each other. In general, they did not appear to be good pollinators with each other. The highest quality of pollen was observed for the following cultivars: 'Schattenmorelle IR-2', 'Koral' and Vowi and the lowest result was obtained in 'újfehértói Fürtos'. The highest yield was given by the following cultivars: Vowi, Schattenmorelle IR-2 and Koral.

  3. Preliminary Screening and Evaluation of Bacterial Wilt-resistance Cherry Tomato Cultivars%抗青枯病樱桃番茄品种的初步筛选及评价

    Institute of Scientific and Technical Information of China (English)

    曾莉莎; 刘建平; 胡规媛; 莫秀文; 杜彩娴; 王红; 梁少丽; 吕顺; 李洪波; 郑汉文

    2016-01-01

    对28个樱桃番茄品种的田间抗病性、植株类型、单果质量、果色、可溶性固形物含量等主要经济性状进行调查。试验结果表明,感病品种红艳发病最重,发病率为73.67%,其次是红箭、红日和金艳,发病率分别为23.33%、21.67%和20.33%,其他品种发病率都在20%以下;28个品种的果实颜色、大小、口感等差异明显,除黑宝石映泰及黄金蛋可溶性固形物含量在4.10%以下外,其他品种可溶性固形物含量都达5.20%以上。综合分析,筛选出绿天使、粉仙、红娘9号、绿圆、格丽贝、红椭圆等11个抗青枯病且经济性状优良的樱桃番茄品种,为优质抗青枯病樱桃番茄的选育和推广提供了参考。%This experiment investigated the main economic characteristics of 28 cherry tomato cultivars including disease resistance, plant type, single fruit weight, fruit color, soluble solid content, etc. The results showed that the sensitive cultivar Hongyan had the highest disease incidence, and its disease incidence was 73.67%, followed by Hongjian, Hongri, and Jinyan with the disease incidence of 23.33%, 21.67% and 20.33% respectively, while disease incidence of other tomatoes cultivars were below 20%. 28 cherry tomato cultivars were obvious differences in fruit color, size, taste, etc. Soluble solid content of most tomato cultivars were more than 5.20% besides Heibaoshiyingtai and Huangjingdan, which soluble solid content were blow 4.10%. Other 11 bacterial wilt-resistance cherry tomato cultivars including Lvtianshi, Fenxian, Hongniang No.9, Lvyuan, Gelibei, Hongtuoyuan and so on with excellent economic characteristics were selected out. This study would provide references for cultivar breeding and promotion of cherry tomato.

  4. Influence of kind of trees on flowering and yielding of sour cherry cultivar Łutówka

    Directory of Open Access Journals (Sweden)

    Stanisław Wociór

    2012-12-01

    Full Text Available In no examined years of the studies were found no significant differences of flowering intensity between the ownoroots trees of the cultivar Łutówka and trees budded on Prunus mahaleb. The kind of trees clearly, but not significantly, affected fruit mass. The growth of the ownroot "in vitro" trees were significantly less than the trees budded on Prunus mahaleb seedling. Fruit set and yielding of the own root trees were higher, but only in one year of study significantly higher.

  5. A collection of European sweet cherry phenology data for assessing climate change

    Science.gov (United States)

    Wenden, Bénédicte; Campoy, José Antonio; Lecourt, Julien; López Ortega, Gregorio; Blanke, Michael; Radičević, Sanja; Schüller, Elisabeth; Spornberger, Andreas; Christen, Danilo; Magein, Hugo; Giovannini, Daniela; Campillo, Carlos; Malchev, Svetoslav; Peris, José Miguel; Meland, Mekjell; Stehr, Rolf; Charlot, Gérard; Quero-García, José

    2016-12-01

    Professional and scientific networks built around the production of sweet cherry (Prunus avium L.) led to the collection of phenology data for a wide range of cultivars grown in experimental sites characterized by highly contrasted climatic conditions. We present a dataset of flowering and maturity dates, recorded each year for one tree when available, or the average of several trees for each cultivar, over a period of 37 years (1978-2015). Such a dataset is extremely valuable for characterizing the phenological response to climate change, and the plasticity of the different cultivars' behaviour under different environmental conditions. In addition, this dataset will support the development of predictive models for sweet cherry phenology exploitable at the continental scale, and will help anticipate breeding strategies in order to maintain and improve sweet cherry production in Europe.

  6. 酸樱桃新品种‘玫丽’%A New Sour Cherry Cultivar ‘Meili'

    Institute of Scientific and Technical Information of China (English)

    蔡宇良; 冯瑛; 邱蓉; 韩宇; 张雪; 宛甜

    2013-01-01

    'Meili' is a natural hybrid of Primus avium and Prunus fruticosa selected by cherry research group of Northwest A & F University and approved by Shaanxi Forestry Variety Examining Committee in 2010, which is suitable for processing. It is arbor and the tree growth vigor is middle, semi-dwarf, the tree height is 2.5 - 3.5 m with a dark brown bark colour. The fruit skin is purplish red. The good characteristic includes early ripening, self-pollination, stress resistance and productive. Average fruit weight is 5 g, total sugar concentration is 7.96%, total acidity is 1.45%, soluble solids is 14.1%, soluble protein content is 1.87% and the juice processing rate of fruit is 86.9%, its colour is red which is a good character for processing. It is suitable for cultivation in the area of south of Weibei highland, middle and south of Shaanxi and Long Hai railway line surrounding area.%‘玫丽’属甜樱桃(Prunus avium)和草原樱桃(Prunus fruticosa)的自然杂交种,是西北农林科技大学樱桃课题组通过实生选育而成的樱桃加工品种.乔木,树势中,半矮化,树冠高达2.5~3.5 m,树皮暗褐色.果实紫红色,早熟,自花授粉,抗逆性、丰产性强.单果质量5 g,总糖为7.96%,总酸度为1.45%,果实可溶性固形物含量14.1%,可溶性蛋白质含量1.87%,果实出汁率达86.9%,汁液红色,适宜加工.适宜渭北南部、关中、陕南及陇海线周边地区栽植.

  7. Cultivars

    Directory of Open Access Journals (Sweden)

    Oleg Blum

    2011-01-01

    Full Text Available Some modern agrochemicals with antioxidant potential were tested for their protective effect against ozone injury using clover and tobacco ozone-sensitive cultivars as model plants subjected to ambient ozone at two sites (Kyiv city in Ukraine and Szarów village in Poland. All used agrochemicals showed partial protective effects against ozone injury on clover and tobacco. Conducted studies confirmed the effectiveness of modern fungicides belonging to strobilurin group as protectants of sensitive crops against ozone damage. The effectiveness of new growth regulators “Emistym C” and “Agrostymulin” was showed for the first time. Out of the studied agrochemicals, fungicide “Strobi” and natural growth regulator “Emistym C” demonstrated the best protective effects. These agrochemicals present promise for further studies of their possible utilization for enhancement of ozone tolerance of sensitive crops.

  8. In-time and in-space tandem mass spectrometry to determine the metabolic profiling of flavonoids in a typical sweet cherry (Prunus avium L.) cultivar from Southern Italy.

    Science.gov (United States)

    Crupi, Pasquale; Genghi, Rosalinda; Antonacci, Donato

    2014-10-01

    This paper presents a comprehensive analytical methodology, based on 'in-time' and 'in-space' tandem mass spectrometry (MS) techniques, to identify and quantify flavonoid compounds in a typical Italian sweet cherry cultivar (cv. Ferrovia). Five anthocyanins, four flavan-3-ols and nine flavonols were determined by means of hyphenated high-performance liquid chromatography - multi-stage MS (HPLC-MS(n)) analyses (MS(n) up to MS(4)), among which quercetin-3-O-rutinoside-7-O-glucoside, kaempferol-3-O-rutinoside-7-O-glucoside, quercetin-3-O-galactosyl-rhamnoside and quercetin-3-O-coumaroylglucoside were tentatively identified in sweet cherries for the first time. Ultrafast HPLC and tandem MS (UHPLC-MS/MS) analyses through multiple reaction monitoring experiments showed that cyanidin-3-O-rutinoside and cyanidin-3-O-glucoside were the main anthocyanins of cv. Ferrovia at maturity. Moreover, consistent levels of catechin and epicatechin as well as quercetin-3-O-rutinoside and kaempferol-3-O-rutinoside were also found. Because flavonoids have been ascribed as potential health-promoting compounds, gathered findings provide new insight into the knowledge of the quali-quantitative profile of these phytochemicals into a widespread fruit such as sweet cherry.

  9. IMPROVING SUBMERGENCE TOLERANCE OF VIETNAMESE RICE CULTIVAR BY MOLECULAR BREEDING

    Directory of Open Access Journals (Sweden)

    Ta-Hong Linh

    2013-12-01

    Full Text Available Submergence stress has caused by climate change is the major hindrance to enhancing rice production of Vietnam. In this study, we have evaluated the levels of submergence tolerance ability of the imported rice cultivars under the 4 different field trials. Among these, IR64-Sub1 exhibits the highest submergence tolerance with stable and high yield, and was used as a donor plant, while Bacthom 7, an elite Vietnamese rice cultivar was used as the recipient plant. In molecular markers study, we have used closely linkage markers with Sub1, flanking markers Sub1, and unlinked marker to Sub1 for the foreground, recombinant and background selections in the backcrossing generations between the donor and the recipient plants. In BC3F1 generation, the individual plant number 116 has carried QTL/Sub1 and retained the highest genetic background of the recipient parent up to 98.6%. The newly improved rice line may be useful for growing in the flooding areas of Vietnam to cope with the climate change.

  10. Genetic Diversity Assessment and Identification of New Sour Cherry Genotypes Using Intersimple Sequence Repeat Markers

    Directory of Open Access Journals (Sweden)

    Roghayeh Najafzadeh

    2014-01-01

    Full Text Available Iran is one of the chief origins of subgenus Cerasus germplasm. In this study, the genetic variation of new Iranian sour cherries (which had such superior growth characteristics and fruit quality as to be considered for the introduction of new cultivars was investigated and identified using 23 intersimple sequence repeat (ISSR markers. Results indicated a high level of polymorphism of the genotypes based on these markers. According to these results, primers tested in this study specially ISSR-4, ISSR-6, ISSR-13, ISSR-14, ISSR-16, and ISSR-19 produced good and various levels of amplifications which can be effectively used in genetic studies of the sour cherry. The genetic similarity among genotypes showed a high diversity among the genotypes. Cluster analysis separated improved cultivars from promising Iranian genotypes, and the PCoA supported the cluster analysis results. Since the Iranian genotypes were superior to the improved cultivars and were separated from them in most groups, these genotypes can be considered as distinct genotypes for further evaluations in the framework of breeding programs and new cultivar identification in cherries. Results also confirmed that ISSR is a reliable DNA marker that can be used for exact genetic studies and in sour cherry breeding programs.

  11. Quality and antioxidant properties on sweet cherries as affected by preharvest salicylic and acetylsalicylic acids treatments.

    Science.gov (United States)

    Giménez, María José; Valverde, Juan Miguel; Valero, Daniel; Guillén, Fabián; Martínez-Romero, Domingo; Serrano, María; Castillo, Salvador

    2014-10-01

    The effects of salicylic acid (SA) or acetylsalicylic acid (ASA) treatments during on-tree cherry growth and ripening on fruit quality attributes, especially those related with the content on bioactive compounds and antioxidant activity were analysed in this research. For this purpose, two sweet cherry cultivars, 'Sweet Heart' and 'Sweet Late', were used and SA or ASA treatments, at 0.5, 1.0 and 2.0mM concentrations, were applied at three key points of fruit development (pit hardening, initial colour changes and onset of ripening). These treatments increased fruit weight and ameliorated quality attributes at commercial harvest, and led to cherries with higher concentration in total phenolics and in total anthocyanins, as well as higher antioxidant activity, in both hydrophilic and lipophilic fractions. Thus, preharvest treatments with SA or ASA could be promising tools to improve sweet cherry quality and health beneficial effects for consumers.

  12. Comparison on Adaptability of New Cherry Tomato Cultivars in Greenhouse of Haiyan County of Zhejiang%浙江海盐设施樱桃番茄新品种适应性比较试验

    Institute of Scientific and Technical Information of China (English)

    姜伟锋; 敖成光; 俞刚翔

    2013-01-01

    The adaptability comparison of eight cherry tomato cultivars, Yafei No.1, Yafei No.2, Yafei No.15, Heifei 20, Fenhongjiali, T1319, PT100501 and Jinzhu, was carried out in Haiyan county, to study their phenological period, plant characters, fruit characters and yield characters. The results showed that the eight cultivars had few differences in phenological period, but their fruit color and yield were quite different. On considering various indexes, Yafei No.1 was suitable to be used as the main planting cultivar of cherry tomato in Haiyan county, and Heifei 20 could be planted appropriately to meet different market demands, while Jinzhu was susceptible to gray mold, and its fruits were easy to crack and tasted generally, so it was unsuitable for further promotion.%  在海盐县引进亚非1号、亚非2号、亚非15号、黑妃20、粉红嘉丽、T1319、PT100501、金珠8个樱桃番茄品种进行适应性比较试验,并对其物候期、植株性状、果实性状、产量性状等进行研究对比。试验结果表明,8个参试品种物候期差异不大,果实颜色各异,产量差异较大。综合各项指标来看,亚非1号可作为海盐县樱桃番茄主栽品种;黑妃20可适量种植,以满足不同市场的需求;金珠易裂果,易感灰霉病,口感一般,建议逐步淘汰。

  13. Cherry Rain

    Institute of Scientific and Technical Information of China (English)

    1997-01-01

    When I am homesick, the cherry trees dotting here and there in my hometown always visit me in my dreams. The cherry tree is a common and special plant. Full of vigor, it grows by itself and needs little care. In early spring, people use simple tools to cut seedlings from the mother root and plant them in soil, then the seedlings start to grow. Three years later, the young plant becomes a big tree and it

  14. Recovery of nitrogen fertilizer by traditional and improved rice cultivars in the Bhutan Highlands

    DEFF Research Database (Denmark)

    Ghaley, Bhim Bahadur; Høgh-Jensen, Henning; Christiansen, Jørgen Lindskrog

    2010-01-01

    The recovery of soil derived nitrogen (NDFS) and fertilizer N (NDFF) was investigated in highland rice (Oryza sativa L.) fields in Bhutan, characterized by high inputs of farmyard manure (FYM). The effect of 60 kg N ha-1 (60 N) applied in two splits to a traditional and an improved cultivar...... greater harvest index (HI). The mean percentage recovery of fertilizer N (REN) applied at 45 days after transplanting (DAT) was 34% compared to 22% at 7 DAT, resulting in 56% greater uptake of NDFF at 45 DAT. The overall REN for both the improved and the traditional cultivars were 25.7% and 30......% respectively, with no difference between cultivars, but REN decreased with increasing FYM inputs. Fertilizer N recommendations that allow for previous FYM inputs combined with applications timed to coincide with maximum crop demand (45 DAT), and the use of improved cultivars, could enhance N fertilizer...

  15. COMPATIBILITY AND FEASIBILITY OF GRAFT TOMATO CULTIVAR SANTA CRUZ KADA IN DIFFERENT ROOTSTOCKS

    Directory of Open Access Journals (Sweden)

    A. R. Zeist

    2015-02-01

    Full Text Available Tomato production through the technique of grafting aims to control soil pathogens, induce flowering, and improve tolerance to waterlogging, salinity and alkalinity of the soil. For this work were performed 50 grafts for each type of rootstock, totaling 100 slips and 50 seedlings kept as control. After 15 days of grafting, the seedlings were evaluated on the percentage of picks grafting. The treatment which used the tomato cultivar Cherry Red® as rootstock presented results of vegetative growth (height and volume Cup higher than other treatments. However after transplanting, defective development was observed for plants with grafting when compared to the controls. After 35 days of follow up, there was a low survival rate, being 5% of the plants. According to the results obtained in this work the tomato cultivar Santa Cruz Kada® has good compatibility with the rootstock cultivar Cayenne® pepper and tomato cultivar Cherry Red®

  16. Clonal stability and epigenetic variation in sour cherry

    DEFF Research Database (Denmark)

    Clausen, Sabine Karin

    Harvested yields of sour cherry (Prunus cerasus) cultivar 'Stevnsbaer' grown in Denmark have been highly variable over the years, yet some propagated derived trees selected from within the two widely grown clones of 'Stevnsbaer' cl. 'Birgitte' and cl. 'Viki' have consistently produced higher yields....... This observed variability within the clones raises the question as to whether more suitable plant material for the future might be found through similar selection. In this study, a number of the selected individuals were evaluated based on their morphological (harvested yield, number of buds/flowers/fruits......, leaf chlorophyll content, cold hardiness and tree structure) and genomic (DNA fingerprinting, nuclear DNA content, DNA methylation) differences to better understand the differences that produced the improved individuals within the existing clones of sour cherry. In addition, it was investigated whether...

  17. Melhoramento genético do cultivar de milho IAC-1 Genetic improvement of the maize cultivars IAC-1

    Directory of Open Access Journals (Sweden)

    Luiz Torres de Miranda

    1978-01-01

    Full Text Available São relatados os resultados obtidos no melhoramento do cultivar de milho IAC-1 pela introdução de linhagens prolíficas nesta população, que já vinha sendo melhorada há oito ciclos pelo método de seleção entre e dentro de famílias de meios irmãos. Para produção de grãos foi observado um progresso genético médio de 3,3% por ciclo, comparado com um progresso genético esperado de 7,2% por ciclo. Para índice-de-espigas foi observado um progresso genético de 8,9% e um progresso genético espe-rado de 1,9% por ciclo. Com a introdução de fatores de prolificidade, o índice-de-espigas aumentou consideravelmente superando inclusive o do híbrido duplo comercial utilizado como controle. Houve pequeno aumento da porcentagem de plantas acamadas e quebra-das, com o decorrer dos ciclos de seleção. Computando-se os ganhos obtidos inicialmente neste cultivar, houve em 10 ciclos de seleção um progresso acumulado de 28% para produção de grãos e de 10% para índice-de-espigas, sendo este último obtido quase exclusivamente após a introdução das linha-gens prolíficas.There are presented results of the improvement of the maize (Zea mays, L. cultivars IAC-1, after the introduction of prolificacy in a population formerly improved for eight cycles by the modified ear-to-row procedure. It was obtained an average progress of 3.3% per cycle in grain yield, against an expected genetic progress of 7.2% per cycle. For the ear index a genetic progress of 8.9% per cycle was observed and an expected genetic progress of 1.9% per cycle was estimated. With the introduction of prolificacy factors, ear index increased considerably outyielding the commercial double hybrid control Lodging changed 'very little with the cycles of selection. The IAC-1 cultivars showed an accumulated progress of 28% for grain yield and 10% for ear index. The gain in ear index is mainly due to the prolific inbred lines introduced in the synthetic.

  18. Genetic Diversity Analysis of Iranian Improved Rice Cultivars through RAPD Markers

    Directory of Open Access Journals (Sweden)

    Ghaffar KIANI

    2011-08-01

    Full Text Available The aim of this study was to evaluate the genetic diversity of Iranian improved rice varieties. Sixteen rice varieties of particular interest to breeding programs were evaluated by means of random amplified polymorphic DNA (RAPD technique. The number of amplification products generated by each primer varied from 4 (OPB-04 to 11 (OPD-11 with an average of 8.2 bands per primer. Out of 49 bands, 33 (67.35% were found to be polymorphic for one or more cultivars ranging from 4 to 9 fragments per primer. The size of amplified fragments ranged between 350 to 1800 bp. Pair-wise Nei and Li�s (1979 similarity estimated the range of 0.59 to 0.98 between rice cultivars. Results illustrate the potential of RAPD markers to distinguish improved cultivars at DNA level. The information will facilitate selection of genotypes to serve as parents for effective rice breeding programs in Iran.

  19. Broccoli Cultivar Performance under Organic and Conventional Management Systems and Implications for Crop Improvement

    NARCIS (Netherlands)

    Renaud, E.N.C.; Lammerts Van Bueren, E.; Caldas Paulo, M.J.; Eeuwijk, van F.A.; Juvik, J.A.; Hutton, M.G.; Myers, J.R.

    2014-01-01

    To determine if present commercial broccoli cultivars meet the diverse needs of organic management systems, such as adaptation to low N input, mechanical weed management, and no chemical pesticide use, and to propose the selection environments for crop improvement for organic production, we compared

  20. Polyphenol content and antioxidant activity of sour cherries from Serbia

    Directory of Open Access Journals (Sweden)

    Mitić Milan N.

    2012-01-01

    Full Text Available The aim of this study is to evaluate the content of phenolics: the total phenols (TP, flavonoids (TF, anthocyanins (TA, as well as the total antioxidant capacity (TAC in three sour cherry cultivars (Prunus cerasus L. introduced to the southeast Serbia climate conditions. Among the researched sour cherries, ‘Oblacinska’ cultivar contained the highest amounts of all groups of phenolics, followed by ‘Cigancica’ > ‘Marela’. A significant difference were observed in the phenolic content among different cultivars and growing seasons (p<0.05, and the phenolic compounds were significantly higher in the growing season 2009. The examined cultivars possess a high antioxidant capacity, and all phenolics of highy correlation with TAC. The following compounds were identified and quantified using HPLC-DAD: 4 anthocyanins, the most abundant of which was cyaniding-3-glucoside in ‘Marela’ and ‘Oblacinska’, and cyanidin-3-glucosylrutinoside in ‘Cigancica’, and 4 hydroxycinnamic acids, the most abundant of which was neochlorogenic acid in all sour cherry cultivars. The growing and ripening process on the tree of sour cherry cv. Oblacinska was evaluated, also. The results showed significant increases in total phenols during the ripening, the total anthocyanins and total antioxidant capacity and 4 quantified anthocyanins, however the neochlorogenic acid decreased during the ripening. The study indicated that the growing and climate conditions in southeast Serbia are convenient for introducing sour cherry cultivars.

  1. A New Fresh-eating Cultivar of Chinese Dwarf Cherry (Cerasus humilis Bunge) ‘ Nongda 7'%鲜食欧李新品种‘农大7号’

    Institute of Scientific and Technical Information of China (English)

    王鹏飞; 曹琴; 杜俊杰; 张建成; 穆霄鹏

    2013-01-01

    'Nongda 7' is a new variety selected from seedlings of Chinese dwarf cherry (Cerasus humilis Bunge) . The fruit is flat round with an orange background and a flush on the sunny side. The flesh color is light yellow and the flesh is freestone. The average fruit weight is 14.3 g with a 94.4% edible ratio, average soluble solids content 14.57%, total sugar content 7.69%. Titratable acidity 1.28%, sugar acid ratio 6.22, vitamin C 506.9 mg · kg-1, total amino acid 5.45 g · kg-1. The fruit is beautiful, fragrant, sweet and sour and the yield is high. Fruits ripen in late August in Jinzhong (Shanxi Province) . It is a cultivar that specially used for fresh-eating.%欧李(Cerasus humilis Bunge)新品种‘农大7号’是从欧李自然杂交实生后代中选育而成.果实扁圆形,底色橘黄色,向阳面着红晕,果肉淡黄色,离核.平均单果质量14.3 g,可食率94.4%,可溶性固形物14.57%,总糖7.96%,可滴定酸1.28%,糖酸比6.22,维生素C 506.9 mg·kg-1,氨基酸总量5.45 g·kg-1.外观漂亮,香味浓郁,酸甜适口,丰产强,在山西晋中8月下旬成熟,为鲜食品种.

  2. Exploring the original causes of cherry cultivar Manaohong originated from Nayong County,Guizhou Province using ISSR markers%利用ISSR标记探讨贵州地方樱桃种质玛瑙红的起源原因

    Institute of Scientific and Technical Information of China (English)

    宋常美; 文晓鹏

    2011-01-01

    In this paper,genetic relationship among local cherry cultivar Manaohong originated from Nayong County of Guizhou Province,as well as:sour cherry,black pearl and four sweet cherry cultivars were investigated using ISSR markers.The purpose was to investigate the causes of variation on Manaohong.A total of 286 markers were scored from 15 primers,which were demonstrated highly reproducible,clear and polymorphic bands.Among the obtained markers,249 were polymorphic,accounting for 87.06% of the total.As analyzed by NTSYS2.01,the genetic similarity among the seven accessions ranged from 0.41 to 0.94,and the genetic similarity between Manaohong and sour cherry was 0.57.Manaohong has significant genetic differences with sour cherry on molecular level,possibly it is a hybridization variation of sour cherry.%为探讨玛瑙红的起源原因,利用ISSR标记对贵州省纳雍县地方樱桃种质玛瑙红、酸樱桃、黑珍珠和4种欧洲甜樱桃等种质的亲缘关系进行分析。结果表明:用15条稳定性强、条带清晰及多态性丰富的引物进行PCR扩增,共获得286个标记位点,其中多态性位点为249个,多态性比例为87.06%;采用NTSYS2.01软件计算,7种樱桃相似性系数为0.41~0.94,玛瑙红与酸樱桃的相似系数为0.57。玛瑙红和酸樱桃在分子水平上的遗传差异很大,该种质可能为酸樱桃的实生变异种质。

  3. Efeito da densidade de plantação e da cultivar no crescimento da cerejeira sobre o porta-enxerto edabriz em quatro locais do norte e centro de Portugal Effect of tree planting density and cultivar on sweet cherry growth onto the edabriz rootstock at four locations in the north and centre of Portugal

    Directory of Open Access Journals (Sweden)

    Alberto Santos

    2009-12-01

    Full Text Available Foi avaliado o crescimento das cultivares de cerejeira Regina, Skeena e Sweetheart até ao final da 2ª folha, sob o efeito do porta enxerto Edabriz e quatro densidades de plantação. O ensaio foi implantado em Março de 2003, em quatro locais do Norte e Centro de Portugal, e compreende duas repetições em Caria, Vila Real e Alcongosta, e três em Carrazedo de Montenegro. A largura de entrelinhas é cerca de 5,0 metros, e as distâncias entre plantas na linha são 70, 140, 210 e 280 cm, a que correspondem densidades de 2600, 1300, 860 e 650 plantas/ha, respectivamente. Anualmente, foi registado o diâmetro do tronco de cada planta e calculada a sua área da secção do tronco (AST. No final da 2ª folha foram já significativas as diferenças de crescimento observadas, tanto ao nível do local como da densidade de plantação. As cerejeiras do ensaio de C. Montenegro cresceram mais 76, 36 e 9% do que as dos ensaios de Alcongosta, Vila Real e Caria, respectivamente, sendo o local responsável por 25% da variância total esperada. A densidade de plantação reteve já 3% da variância total, tendo as árvores deixadas a 70 cm na linha crescido menos 22% do que as mais afastadas. Por conseguinte, em Alcongosta e Vila Real as cultivares cresceram muito pouco neste porta-enxerto, pelo que em condições análogas é importante ajustar as dotações hídricas e nutricionais às necessidades específicas do porta-enxerto, de forma a melhor gerir o crescimento vegetativo e preparar as árvores para a sua função primordial: a produção.The growth of sweet cherry cultivars Regina, Skeena and Sweetheart was evaluated when grafted onto the Edabriz rootstock and at four planting densities. The trial was set on March 2003 at four locations in the North and Centre of Portugal, with two replications in Caria, Vila Real and Alcongosta and three in Carrazedo de Montenegro. Within row spacing is approximately 5,0 m and plant spacing along the row is 0.7, 1

  4. Montmorency Tart cherries (Prunus cerasus L.) modulate vascular function acutely, in the absence of improvement in cognitive performance.

    Science.gov (United States)

    Keane, K M; Haskell-Ramsay, C F; Veasey, R C; Howatson, G

    2016-12-01

    Cerebral blood volume and metabolism of oxygen decline as part of human ageing, and this has been previously shown to be related to cognitive decline. There is some evidence to suggest that polyphenol-rich foods can play an important role in delaying the onset or halting the progression of age-related health disorders such as CVD and Alzheimer's disease and to improve cognitive function. In the present study, an acute, placebo-controlled, double-blinded, cross-over, randomised Latin-square design study with a washout period of at least 14 d was conducted on twenty-seven, middle-aged (defined as 45-60 years) volunteers. Participants received either a 60 ml dose of Montmorency tart cherry concentrate (MC), which contained 68·0 (sd 0·26) mg cyanidin-3-glucoside/l, 160·75 (sd 0·55) mean gallic acid equivalent/l and 0·59 (sd 0·02) mean Trolox equivalent/l, respectively, or a placebo. Cerebrovascular responses, cognitive performance and blood pressure were assessed at baseline and 1, 2, 3 and 5 h following consumption. There were significant differences in concentrations of total Hb and oxygenated Hb during the task period 1 h after MC consumption (P≤0·05). Furthermore, MC consumption significantly lowered systolic blood pressure (P≤0·05) over a period of 3 h, with peak reductions of 6±2 mmHg at 1 h after MC consumption relative to the placebo. Cognitive function and mood were not affected. These results show that a single dose of MC concentrate can modulate certain variables of vascular function; however, this does not translate to improvements in cognition or mood.

  5. Repellent effects of various cherry tomato accessions on the two-spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae).

    Science.gov (United States)

    Lucini, T; Resende, J T V; Oliveira, J R F; Scabeni, C J; Zeist, A R; Resende, N C V

    2016-01-01

    Several studies have been conducted on resistance sources to improve the genetic resistance of farm-grown tomatoes to arthropod pests, including phytophagous mites. In the present study, we evaluate the behavior of the two-spotted spider mite on different cherry tomato accessions to identify possible sources of resistance (repellent effect) to this pest. Sixty-four accessions of cherry tomatoes, Solanum lycopersicum var. cerasiforme (Dunal), were tested. In addition, a commercial cultivar of cherry tomato cv. Sweet Grape (susceptible pattern) and the wild tomato accession Solanum pennellii Correll LA-716 (multiple pest resistance) were evaluated as well. The distance traveled by mites on the leaflet surface over time varied largely among cherry tomato accessions. The wild genotype, S. pennellii LA-716, showed the smallest traveled distance on the leaflet surface (0.8 to 1.1 mm over time), and the variety cv. Sweet Grape was one of the genotypes with highest traveled distance (16.2 to 16.4 mm over time). The cherry tomato accessions 2298-42, RVTC-03, and 6889-53 showed a decrease in the traveled distance by mites over time, similar to that as observed in the wild tomato accession LA716. These accessions showed mite repellence levels similar to those of the wild genotype and may, therefore, be good candidates for breeding programs dealing with resistance to mites.

  6. Tart cherry supplementation improves working memory, hippocampal inflammation and autophagy in aged rats

    Science.gov (United States)

    High consumption of fruits and vegetables has been associated with reduced risk of debilitating diseases and improved cognition in aged populations. These beneficial effects have been attributed to the antioxidant/anti-inflammation properties of phytochemicals found in fruits and vegetables. Tart ch...

  7. NOTE - Genetic improvement of vegetables: development of open-pollinated cultivars

    Directory of Open Access Journals (Sweden)

    Paulo César Tavares de Melo

    2011-01-01

    Full Text Available The estimated market value for vegetable seeds in Brazil, based on prices paid by producers, reached around R$ 300million in 2007. Seeds from open-pollinated cultivars accounted for only 18 %. This data clearly indicates the changeover fromopen-pollinated to hybrid seeds in recent decades in the main varietal segments of the Brazilian vegetable market. This lecture willoutline a historical retrospective of the pioneer activities of genetic improvement of vegetable crops targeting open-pollinatedcultivars in breeding programs conducted by the public universities and research institutes and their impact on the development ofthe Brazilian horticulture. The current situation, challenges and future prospects will also be discussed.

  8. Soluble and bound hydroxycinnamates in coffee pulp (Coffea arabica) from seven cultivars at three ripening stages.

    Science.gov (United States)

    Rodríguez-Durán, Luis V; Ramírez-Coronel, Ma Ascención; Aranda-Delgado, Eduardo; Nampoothiri, K Madhavan; Favela-Torres, Ernesto; Aguilar, Cristóbal N; Saucedo-Castañeda, Gerardo

    2014-08-01

    The contents of soluble and bound hydroxycinnamates (HCAs) were analyzed in coffee pulp (CP) of seven cultivars of Coffea arabica at three different ripening stages. Methodologies for the extraction and analysis of HCAs were evaluated and improved. HCAs were present mainly in the soluble fraction (68-97%). Chlorogenic acid was the main phenolic acid (94-98%) in the soluble fraction, whereas caffeic acid was the most abundant HCA found in the bound fraction (72-88%). Small amounts of free and bound ferulic and p-coumaric acids were also detected. The content of total HCAs in CP reached the maximum concentration at the semiripe stage (7.4-25.5 mg/g CP, dw) but decreased at the ripe stage for six of the seven cultivars. These findings suggest that unripe or semiripe coffee cherries, considered as defective cherries, are a potential inexpensive source of phenolic compounds, such as chlorogenic and caffeic acids.

  9. Cherry-Slush-Candling Apparatus

    Science.gov (United States)

    Stephens, James B.; Weiss, James R.; Hoover, Gordon

    1996-01-01

    Proposed infrared-scanning apparatus for use in bakeries making cherry pies detect cherry pits remaining in cherry slush after pitting process. Pits detected via their relative opacity to infrared radiation.

  10. Ancestral QTL alleles from wild emmer wheat improve drought resistance and productivity in modern wheat cultivars

    Directory of Open Access Journals (Sweden)

    Lianne eMerchuk-Ovnat

    2016-04-01

    Full Text Available Wild emmer wheat (Triticum turgidum ssp. dicoccoides is considered a promising source for improving stress resistances in domesticated wheat. Here we explored the potential of selected quantitative trait loci (QTLs from wild emmer wheat, introgressed via marker-assisted selection, to enhance drought resistance in elite durum (T. turgidum ssp. durum and bread (T. aestivum wheat cultivars. The resultant near-isogenic lines (BC3F3 and BC3F4 were genotyped using SNP array to confirm the introgressed genomic regions and evaluated in two consecutive years under well-watered (690–710 mm and water-limited (290–320 mm conditions. Three of the introgressed QTLs were successfully validated, two in the background of durum wheat cv. Uzan (on chromosomes 1BL and 2BS, and one in the background of bread wheat cvs. Bar Nir and Zahir (chromosome 7AS. In most cases, the QTL x environment interaction was validated in terms of improved grain yield and biomass - specifically under drought (7AS QTL in cv. Bar Nir background, under both treatments (2BS QTL, and a greater stability across treatments (1BL QTL. The results provide a first demonstration that introgression of wild emmer QTL alleles can enhance productivity and yield stability across environments in domesticated wheat, thereby enriching the modern gene pool with essential diversity for the improvement of drought resistance.

  11. Ancestral QTL Alleles from Wild Emmer Wheat Improve Drought Resistance and Productivity in Modern Wheat Cultivars

    Science.gov (United States)

    Merchuk-Ovnat, Lianne; Barak, Vered; Fahima, Tzion; Ordon, Frank; Lidzbarsky, Gabriel A.; Krugman, Tamar; Saranga, Yehoshua

    2016-01-01

    Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is considered a promising source for improving stress resistances in domesticated wheat. Here we explored the potential of selected quantitative trait loci (QTLs) from wild emmer wheat, introgressed via marker-assisted selection, to enhance drought resistance in elite durum (T. turgidum ssp. durum) and bread (T. aestivum) wheat cultivars. The resultant near-isogenic lines (BC3F3 and BC3F4) were genotyped using SNP array to confirm the introgressed genomic regions and evaluated in two consecutive years under well-watered (690–710 mm) and water-limited (290–320 mm) conditions. Three of the introgressed QTLs were successfully validated, two in the background of durum wheat cv. Uzan (on chromosomes 1BL and 2BS), and one in the background of bread wheat cvs. Bar Nir and Zahir (chromosome 7AS). In most cases, the QTL x environment interaction was validated in terms of improved grain yield and biomass—specifically under drought (7AS QTL in cv. Bar Nir background), under both treatments (2BS QTL), and a greater stability across treatments (1BL QTL). The results provide a first demonstration that introgression of wild emmer QTL alleles can enhance productivity and yield stability across environments in domesticated wheat, thereby enriching the modern gene pool with essential diversity for the improvement of drought resistance. PMID:27148287

  12. Ancestral QTL Alleles from Wild Emmer Wheat Improve Drought Resistance and Productivity in Modern Wheat Cultivars.

    Science.gov (United States)

    Merchuk-Ovnat, Lianne; Barak, Vered; Fahima, Tzion; Ordon, Frank; Lidzbarsky, Gabriel A; Krugman, Tamar; Saranga, Yehoshua

    2016-01-01

    Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is considered a promising source for improving stress resistances in domesticated wheat. Here we explored the potential of selected quantitative trait loci (QTLs) from wild emmer wheat, introgressed via marker-assisted selection, to enhance drought resistance in elite durum (T. turgidum ssp. durum) and bread (T. aestivum) wheat cultivars. The resultant near-isogenic lines (BC3F3 and BC3F4) were genotyped using SNP array to confirm the introgressed genomic regions and evaluated in two consecutive years under well-watered (690-710 mm) and water-limited (290-320 mm) conditions. Three of the introgressed QTLs were successfully validated, two in the background of durum wheat cv. Uzan (on chromosomes 1BL and 2BS), and one in the background of bread wheat cvs. Bar Nir and Zahir (chromosome 7AS). In most cases, the QTL x environment interaction was validated in terms of improved grain yield and biomass-specifically under drought (7AS QTL in cv. Bar Nir background), under both treatments (2BS QTL), and a greater stability across treatments (1BL QTL). The results provide a first demonstration that introgression of wild emmer QTL alleles can enhance productivity and yield stability across environments in domesticated wheat, thereby enriching the modern gene pool with essential diversity for the improvement of drought resistance.

  13. Seed washing, exogenous application of gibberellic acid, and cold stratification enhance the germination of sweet cherry (Prunus avium L.) seed

    NARCIS (Netherlands)

    Javanmard, T.; Zamani, Z.; Keshavarz Afshar, R.; Hashemi, M.; Struik, P.C.

    2014-01-01

    Seed germination in sweet cherry (Prunus avium L.) is a slow and lengthy process which has delayed breeding efforts. In this study, seed from ripe fruit of the sweet cherry cultivar ‘Lambert’ were collected and, after removing the endocarp, various dormancy-breaking treatments such as seed washing,

  14. An improved Agrobacterium-mediated transformation of recalcitrant indica rice (Oryza sativa L.) cultivars.

    Science.gov (United States)

    Shri, Manju; Rai, Arti; Verma, Pankaj Kumar; Misra, Prashant; Dubey, Sonali; Kumar, Smita; Verma, Sikha; Gautam, Neelam; Tripathi, Rudra Deo; Trivedi, Prabodh Kumar; Chakrabarty, Debasis

    2013-04-01

    Agrobacterium-mediated transformation of indica rice varieties has been quite difficult as these are recalcitrant to in vitro responses. In the present study, we established a high-efficiency Agrobacterium tumefaciens-mediated transformation system of rice (Oryza sativa L. ssp. indica) cv. IR-64, Lalat, and IET-4786. Agrobacterium strain EHA-101 harboring binary vector pIG121-Hm, containing a gene encoding for β-glucuronidase (GUS) and hygromycin resistance, was used in the transformation experiments. Manipulation of different concentrations of acetosyringone, days of co-culture period, bacterial suspension of different optical densities (ODs), and the concentrations of L-cysteine in liquid followed by solid co-culture medium was done for establishing the protocol. Among the different co-culture periods, 5 days of co-culture with bacterial cells (OD600 nm = 0.5-0.8) promoted the highest frequency of transformation (83.04 %) in medium containing L-cysteine (400 mg l(-1)). Putative transformed plants were analyzed for the presence of a transgene through genomic PCR and GUS histochemical analyses. Our results also suggest that different cultural conditions and the addition of L-cysteine in the co-culture medium improve the Agrobacterium-mediated transformation frequencies from an average of 12.82 % to 33.33 % in different indica rice cultivars.

  15. Sweet and sour cherry decorative forms

    Directory of Open Access Journals (Sweden)

    Ljubojević Mirjana

    2012-01-01

    Full Text Available Biodiversity of natural populations, biodiversity located on farm and the introduced cultivars and selections are a rich source of genetic variability in sour and sweet cherries, but they have never been bread with the aim of creating decorative varieties. Low vigour − dwarfing and upright − sour cherry genotypes, NS 1/16 KK and NS 1/24 KK, were selected from natural population of Fruška Gora and private arboretum, respectively. Sweet cherry selections NS 6/15 K and RŠ 8/27 were part of on farm conservation of genetic resources. Furthermore, reduction in vigour was achieved by defining specific combining abilities as a result of rootstock/scion interaction. The outcome of this study is unique columnar and dwarf forms that integrate specific genetic potential of varieties and selections, their interaction with rootstocks and traditional horticultural skills. Collected biodiversity is another confirmation that the Balkan peninsula is one of the most valuable secondary centres of genetic diversity and inexhaustible gene pool for breeding both, varieties and vegetative rootstocks.

  16. An improved protocol for efficient transformation and regeneration of diverse indica rice cultivars

    Directory of Open Access Journals (Sweden)

    Sahoo Khirod K

    2011-12-01

    Full Text Available Abstract Background Rice genome sequencing projects have generated remarkable amount of information about genes and genome architecture having tremendous potential to be utilized in both basic and applied research. Success in transgenics is paving the way for preparing a road map of functional genomics which is expected to correlate action of a gene to a trait in cellular and organismal context. However, the lack of a simple and efficient method for transformation and regeneration is a major constraint for such studies in this important cereal crop. Results In the present study, we have developed an easy, rapid and highly efficient transformation and regeneration protocol using mature seeds as explants and found its successful applicability to a choice of elite indica rice genotypes. We have optimized various steps of transformation and standardized different components of the regeneration medium including growth hormones and the gelling agent. The modified regeneration medium triggers production of large number of shoots from smaller number of calli and promotes their faster growth, hence significantly advantageous over the existing protocols where the regeneration step requires maximum time. Using this protocol, significantly higher transformation efficiency (up to 46% and regeneration frequency (up to 92% for the untransformed calli and 59% for the transformed calli were achieved for the four tested cultivars. We have used this protocol to produce hundreds of independent transgenic lines of different indica rice genotypes. Upon maturity, these transgenic lines were fertile thereby indicating that faster regeneration during tissue culture did not affect their reproductive potential. Conclusions This speedy, yet less labor-intensive, protocol overcomes major limitations associated with genetic manipulation in rice. Moreover, our protocol uses mature seeds as the explant, which can easily be obtained in quantity throughout the year and kept

  17. Comparative study of phenolic compounds and antioxidant activity in different species of cherries.

    Science.gov (United States)

    Liu, Yun; Liu, Xinyan; Zhong, Fei; Tian, Rongrong; Zhang, Kaichun; Zhang, Xiaoming; Li, Tianhong

    2011-05-01

    A new spectrometric method ultra performance liquid chromatography-tandem mass spectrometric with high precision and rapid analysis was developed to separate 17 phenolic compounds. Different species of cherries, including 10 sweet cherry (Prunus avium L.) cultivars, a tart cherry (P. cerasus L.) rootstock (CAB), and a hybrid rootstock 'Colt' (P. avium × P. pseudocerasus), were analyzed for phenolics contents by this method. The results showed that significant differences were observed among the phenolic compound contents in different cherry species. In 10 sweet cherry cultivars, the contents of neochlorogenic acid and cyanidin-3O-rutinoside were much higher in red-colored fruits (for example, 64.60 and 44.50 mg/100 g fresh weight in Burlat, respectively) than those in bicolored ones. Principal component analysis revealed that cyanidin-3O-rutinoside was an effective index for grouping the cultivars with similar species and fruit colors. Moreover, there were strong positive correlations between phenolics content and antioxidant activity, which was higher in red-colored cherries.

  18. Black raspberry genomic and genetic resource development to enable cultivar improvement

    Science.gov (United States)

    This project incorporates use of phenotypic, genotypic and genomic data to advance and streamline identification of traits of economic interest and to develop molecular markers for marker assisted breeding of black raspberry (Rubus occidentalis L.). A lack of adapted, disease resistant cultivars has...

  19. Control of Cherry Leaf Spot and Cherry Fruit Fly at Sour Cherry

    Directory of Open Access Journals (Sweden)

    Maria BOROVINOVA

    2015-06-01

    Full Text Available The investigations were made in the experimental sour cherry orchard from the Institute of Agriculture, Kyustendil, Bulgaria, during the period 2010-2014, in order to compare conventional and integrated sour cherry protection against cherry leaf spot and cherry fruit fly. Two variants were investigated, with two different treatment approaches for the control of cherry leaf spot and cherry fruit fly. Variant 1 – cherry leaf spot was controlled by protective treatments with dodin and tebuconazole + trifloxystrobin and cherry fruit fly was controlled by treatments with deltametrin and thiacloprid, independently of density. Variant 2 - cherry leaf spot was controlled by post-infection (curative treatments with tebuconazole + trifloxystrobin and cherry fruit fly was controlled by treatments based on biological threshold: 10-11 cherry fruit fly females caught in traps up to the moment for chemical treatment. It was established that Blumeriella japii can be successfully controlled by post-infection treatments and by this the number of insecticide treatments was reduced. The treatments against cherry fruit fly can be avoided or reduced when the attack control is based on the biological threshold established in the studied area.

  20. Construction of an intra-specific sweet cherry (Prunus avium L.) genetic linkage map and synteny analysis with the Prunus reference map

    Science.gov (United States)

    Linkage maps of the sweet cherry cultivar ‘Emperor Francis’ (EF) and the wild forest cherry ‘New York 54’ (NY) were constructed using primarily simple sequence repeat (SSR) markers and gene-derived markers with known positions on the Prunus reference map. The success rate for identifying SSR markers...

  1. Analysis of pomological traits in new promising sweet cherry genotypes

    Directory of Open Access Journals (Sweden)

    Fotirić-Akšić Milica

    2013-01-01

    Full Text Available Sweet cherry breeding programs are interested in developing new cultivars that are very early or very late in ripening, high yielding, with excellent fruit size and quality. The aim of this study was to evaluate new, promising, sweet cherry genotypes (G-1 and G-2 for ripening time, fruit morphology and fruit chemical content, and compare it with the standard sweet cherry cultivars (Bigarreau Jaboulay, B. H. Burlat, Seneca, Hedelfiger Riesenkirsche, Germersdorfer Grosse Kirsche, Emperor Francis and Bing. Experiment was done during four consecutive years (2006-2009 in Grocka, near Belgrade. The earliest fruit ripening was recorded in G-2 (22nd April while the latest in `Bing` (2nd June. Fruit weight varied from 5.83 g (G-2 up to 8.93 g (G-1. Soluble solid content and total sugar content were the lowest in genotype G-2 (10.48%; 8.45%, respectively but the highest in Emperor Francis (18.28%; 15.98%, respectively. No matter the fact that some standard cultivars showed better results for some traits, genotypes G-1 and G-2 are considered very promising. So, genotype G-1 could be intended for table consumption since it ripens 2-3 days after cv. B. H. Burlat, but have much higher fruit weight. Also, genotype G-2, the earliest in this trial, showed satisfactory fruit weight for this ripening time, which makes it very interesting for fresh market production. [Projekat Ministarstva nauke Republike Srbije, br. 31063 i br. 31038

  2. Molecular analysis of East Anatolian traditional plum and cherry accessions using SSR markers.

    Science.gov (United States)

    Öz, M H; Vurgun, H; Bakir, M; Büyük, İ; Yüksel, C; Ünlü, H M; Çukadar, K; Karadoğan, B; Köse, Ö; Ergül, A

    2013-01-01

    We conducted SSR analyses of 59 accessions, including 29 traditional plum (Prunus domestica), 24 sweet cherry (Prunus avium), and 1 sour cherry (Prunus cerasus) selected from East Anatolian gene sources and 3 plum and 2 cherry reference accessions for molecular characterization and investigation of genetic relationships. Eight SSR loci [1 developed from the apricot (UDAp-404), 4 from the peach (UDP96-010, UDP96-001, UDP96-019, Pchgms1) and 3 from the cherry (UCD-CH13, UCD-CH17, UCD-CH31) genome] for plum accessions and 9 SSR loci [5 developed from the cherry (PS12A02, UCD-CH13, UCD-CH17, UCD-CH31, UCD-CH21), 3 from the peach (Pchgms1, UDP96-001, UDP96-005) and 1 from the plum (CPSCT010) genome] for cherry accessions were used for genetic identification. A total of 66 and 65 alleles were obtained in the genetic analyses of 31 plum and 28 cherry accessions, respectively. The number of alleles revealed by SSR analysis ranged from 4 to 14 alleles per locus, with a mean value of 8.25 in plum accessions, and from 5 to 10 alleles per locus with a mean value of 7.2 in cherry accessions. Only one case of synonym was identified among the cherry accessions, while no case of synonym was observed among the plum accessions. Genomic SSR markers used in discrimination of plum and cherry accessions showed high cross-species transferability in the Prunus genus. Because of their appreciable polymorphism and cross species transferability, the SSR markers that we evaluated in this study will be useful for studies involving fingerprinting of cherry and plum cultivars.

  3. 7 CFR 930.3 - Cherries.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Cherries. 930.3 Section 930.3 Agriculture Regulations... Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES OF... Definitions § 930.3 Cherries. Cherries means all tart/sour cherry varieties grown in the production...

  4. Phenolic profiles of cherry tomatoes as influenced by hydric stress and rootstock technique.

    Science.gov (United States)

    Sánchez-Rodríguez, Eva; Ruiz, Juan M; Ferreres, Federico; Moreno, Diego A

    2012-09-15

    The aim of the present work was to evaluate the influence of genetic and technological factors (cultivar and grafting) combined with the abiotic stress (water stress) on the content of phenolic compounds (flavonoids and phenolic acids classes and total phenolics) in cherry tomato. The identification and contents of phenolic compounds and flavonoids were determined by HPLC-MS in fruits on reciprocal grafting between a drought-tolerant cultivar (Zarina) and drought-sensible cultivar (Josefina), compared with both self-grafted and non-grafted plants. The results show how pre-harvest factors, such as grafting and water stress, can influence the phenolic content of tomato fruits. Phenolic compounds, including rutin that belongs to the group of flavonoids, display a remarkable array of biological and pharmacological activities. So, the richness of ZarxJos under water stress conditions with these compounds confirms its nutritional value and it can be used as a potential source of phenols. The results suggest that grafting on specific rootstocks more adapted to water stress conditions may be a tool to improve crop quality under artificially imposed mild water stress.

  5. Physical and Flavor Profiles of Arabica Coffee as Affected by Cherry Storage Before Pulping

    Directory of Open Access Journals (Sweden)

    Yusianto .

    2014-08-01

    Full Text Available Harvesting and pulping process of coffee cherry in the same day is inaccesible. Storage of coffee cherry before pulping was carried out incorrectly. Some storage treatments before pulping of Arabica coffee cherry had been examined at Indonesian Coffee and Cocoa Research Institute using Arabica coffee cherries from Andungsari Experimental Garden, Bondowoso, East Java. Treatments of the experiment were method and period of cherry storage. Methods of coffee cherry storage were put in plastic sacks; immerse in water, without water replacement; and immerse in water with daily water replacement. Period of coffee cherry storage were 0, 1, 2, 3, 4, 5, 6, and 7 days. After storage treatments, the coffee cherries were pulped, fermented, washed, sundried, and dehulled. The experiment were carried out using randomized block design with three replications. Observation of coffee cherry during storage periods was done on the physical and temperature. Observation of the green coffee were done on the color dan bulk density. The green coffee were roasted at medium roast level for sensory analysis. Observation of roasting profile were out-turn, bulk density and pH of roasted coffee. Sensory analysis used Specialty Coffee Association of America method. Methods and period of cherry storage before pulping significanly influence on the cherry color, parchment color, green coffee color, and the flavor profile of Arabica coffee. Color of dry parchment changed to be red-brown becouse of cherry immersed in water for two days or more. In plastic sacks, Arabica coffee cherry may be stored only for two days, but underwater with or without water replacement, should be not more than five days. Green and sensory quality of Arabica coffee will be deteriorated after five days storage underwater. Coffee cherry storage immerse in water with daily replacing water may improve sensory quality of Arabica coffee.Key word: Arabica coffe, storage, pulping, flavor, physical

  6. Morphological Characterization of Cherry Rootstock Candidates Selected from Central and East Black Sea Regions in Turkey

    Directory of Open Access Journals (Sweden)

    Aysen Koc

    2013-01-01

    Full Text Available The use of rootstocks particularly for sweet cherry cultivars is of great importance for successful and sustainable production. Choosing the right cherry rootstocks is just as important as choosing the right cultivar. In this study, 110 sweet cherry, 30 sour cherry, and 41 mahaleb types displaying rootstock potential for sweet cherry cultivars were selected from Central and East Black Sea Regions in Turkey. The morphologic characteristics of the studied genotypes were compared with the standard clonal rootstocks PHL-A, MaxMa 14, Montmorency, Weiroot 158, Gisela 5, Gisela 6, and SL 64. A total of 42 morphological UPOV characteristics were evaluated in the selected genotypes and clonal rootstocks. The obtained data were analyzed by using principal component analysis and it revealed that eigenvalues of the first 3 components were able to represent 36.43% of total variance. The most significant positive correlations of the plant vigor were determined with leaf blade length and petiole thickness. According to the diversity analysis of coefficients, the 05 C 002 and 08 C 039 genotypes were identified as being similar (6.66, while the 05 C 002 and 55 S 012 genotypes were determined as the most distant genotypes (325.84 in terms of morphology.

  7. Cracking in sweet cherries: A comprehensive review from a physiological, molecular, and genomic perspective

    Directory of Open Access Journals (Sweden)

    Cristián Balbontín

    2013-03-01

    Full Text Available Rain-induced cracking in fruits of sweet cherry (Prunus avium L. is a problem in most producing areas of the world and causes significant economic losses. Different orchard management practices have been employed to reduce the severity of this problem, although a complete solution is not yet available. Fruit cracking is a complex phenomenon and there are many factors that seem to be involved in its development. During the last decade, genomic and biochemical approaches have provided new insights on the different mechanisms that could be involved in the differential susceptibility shown by commercial cultivars. For instance, sweet cherry genome and transcriptome sequencing information have provided new opportunities to study the expression and structure of genes involved in cracking, which may help in the development of new tolerant cultivars. The present review summarizes, discuss, and integrate most of the recently generated information in cultural practices, physiology, biochemistry, and genetics in relation to cracking in sweet cherries.

  8. Effect of ripeness and postharvest storage on the phenolic profiles of cherries (Prunus avium L.)

    DEFF Research Database (Denmark)

    Goncalves, B.; Landbo, Anne-Katrine Regel; Knudsen, D.;

    2004-01-01

    The phenolic compounds hydroxycinnamates, anthocyanins, flavonols, and flavan-3-ols of sweet cherry cultivars Burial, Saco, Summit, and Van harvested in 2001 and 2002 were quantified by HPLC-DAD. Phenolics were analyzed at partially ripe and ripe stages and during storage at 15+/-5 degreesC (room...

  9. Preliminary results on seasonal changes in flower bud cold hardiness of sour cherry

    DEFF Research Database (Denmark)

    Liu, Guangping; Pagter, Majken; Andersen, Lillie

    2012-01-01

    Irregular yields of Prunus cerasus ‘Stevnsbaer Birgitte’, which is the preferred sour cherry cultivar in Denmark, have been proposed to be a result of freeze injury to floral tissues. This study determined floral mortality of P. cerasus ‘Stevnsbaer Birgitte’ and the supposedly more cold hardy P...

  10. 7 CFR 923.5 - Cherries.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Cherries. 923.5 Section 923.5 Agriculture Regulations... Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE SWEET CHERRIES GROWN IN DESIGNATED COUNTIES IN WASHINGTON Order Regulating Handling Definitions § 923.5 Cherries. Cherries means all varieties...

  11. Phosphoenolpyruvate carboxykinase in cherry (Prunus avium L.) fruit during development.

    Science.gov (United States)

    Walker, Robert P; Battistelli, Alberto; Moscatello, Stefano; Chen, Zhi-Hui; Leegood, Richard C; Famiani, Franco

    2011-11-01

    In this study the abundance and location of phosphoenolpyruvate carboxykinase (PEPCK) was determined in the flesh and skin of the sweet cherry (Prunus avium L.) cultivar Durone Nero II during development. PEPCK was not present in young fruit but appeared in both tissues as the fruit increased in size. In these there was no net dissimilation of malic acid, which accounts for the bulk of their organic acid contents when PEPCK was present. To assist in understanding the function of PEPCK, the abundance of a number of other enzymes was determined. These enzymes were aspartate aminotransferase (AspAT), glutamine synthetase (GS), phosphoenolpyruvate carboxylase (PEPC), pyruvate, orthophosphate dikinase (PPDK), and ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco). A potential role for PEPCK in the regulation of pH and the utilization of malate in gluconeogenesis in the flesh and skin of cherries is presented.

  12. CAPS markers improved by cluster-specific amplification for identification of octoploid strawberry (Fragaria x ananassa Duch.) cultivars, and their disomic inheritance.

    Science.gov (United States)

    Kunihisa, M; Fukino, N; Matsumoto, S

    2005-05-01

    Cleavage amplified polymorphic sequence (CAPS) markers of strawberry (Fragaria x ananassa Duch.) can be useful for identifying mislabeled or patent-infringing cultivars in the marketplace. However, CAPS markers in octoploid strawberry tend to give unclear bands because multiple homologous sites are simultaneously amplified by the non-selective PCR. To overcome this problem, we used "cluster-specific amplification" based on the nucleotide sequences of PCR products and were able to improve the band clarity of 18 CAPS markers. By analyzing the marker segregation ratio, we demonstrated that 13 clarified markers were derived from single diploid loci that were transmitted to progeny in a manner consistent with Mendelian inheritance. We discuss the genomic structure of octoploid strawberry from the viewpoint of cluster and segregation analysis and suggest that it comprises independent genomes. We tested the utility of all of the markers we developed for cultivar identification and confirmed their ability to distinguish among 64 strawberry cultivars.

  13. Main viruses in sweet cherry plantations of Central-Western Spain

    Directory of Open Access Journals (Sweden)

    Rodrigo Pérez Sánchez

    2015-02-01

    Full Text Available Sweet cherry trees (Prunus avium L. are susceptible to a range of diseases, but there have been no studies to date about the viral infection of sweet cherry trees in Spain. To determine the phytosanitary status of Spanish sweet cherry plantations, the incidence and leaf symptoms induced by Prune dwarf (PDV, Prunus necrotic ringspot (PNRSV and Apple chlorotic leaf spot (ACLSV viruses were investigated during 2009. Young leaf samples were taken from 350 sweet cherry trees, corresponding to 17 cultivars, and were analysed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA. To associate the leaf symptoms with the virus, 50 mature leaves from each infected tree were visually inspected during the summer. The ELISA results revealed that 72 % of sweet cherry trees were infected by at least one of the viruses. PDV occurred in all sampled cultivars and presented the highest infection rate, followed by ACLSV and PNRSV. A high number of trees showed asymptomatic, in both single and mixed infections. The leaf symptoms associated with the viruses involved generalized chlorosis around the midvein (PDV, chlorotic and dark brown necrotic ringspots on both secondary veins and intervein regions (PNRSV, chlorotic and reddish necrotic ringspots (ACLSV and generalized interveinal chlorosis (PDV-PNRSV.

  14. Differential Responses of Two Broccoli (Brassica oleracea L. var Italica Cultivars to Salinity and Nutritional Quality Improvement

    Directory of Open Access Journals (Sweden)

    Chokri Zaghdoud

    2012-01-01

    Full Text Available The comparative responses of two broccoli cultivars (Brassica oleracea var. Italica, cv. Parthenon and cv. Naxos to a 15 d exposure to different NaCl levels were investigated. Salinity led to increased concentrations of Na+ and Cl− ions in both cultivars, a disruption of the endogenous minerals levels in the shoots and roots—that varied with the cultivar and salt concentration—and decreases in the osmotic potential (Ψπ, root hydraulic conductance (L0, and stomatal conductance (Gs. The reduced biomass of Naxos at moderate NaCl indicates greater sensitivity to salinity, compared with Parthenon. Parthenon accumulated more soluble sugars, for osmotic adjustment, whereas Naxos accumulated proline, which gave the two cultivars differing nutritional characteristics. The total glucosinolates (GSLs content was not affected by salinity in Parthenon while it decreased significantly in Naxos as a consequence of the decrease in the indole GSL. However, Naxos accumulated more aliphatic GSLs under salt stress than Parthenon, which confers on this cultivar a greater nutritional value when cultivated under salinity.These results suggest that, at distinct salinity levels, each broccoli cultivar adopts a specific strategy, indicating the crucial role of the genetic background on the organoleptic and nutritional properties that each cultivar acquires.

  15. Chemical Characterization of Fruit Wine Made from Oblačinska Sour Cherry

    Directory of Open Access Journals (Sweden)

    Milica Pantelić

    2014-01-01

    Full Text Available This paper was aimed at characterizing the wine obtained from Oblačinska, a native sour cherry cultivar. To the best of our knowledge, this is the first paper with the most comprehensive information on chemical characterization of Oblačinska sour cherry wine. The chemical composition was characterized by hyphenated chromatographic methods and traditional analytical techniques. A total of 24 compounds were quantified using the available standards and another 22 phenolic compounds were identified based on the accurate mass spectrographic search. Values of total phenolics content, total anthocyanin content, and radical scavenging activity for cherry wine sample were 1.938 mg gallic acid eqv L−1, 0.113 mg cyanidin-3-glucoside L−1, and 34.56%, respectively. In general, cherry wine polyphenolics in terms of nonanthocyanins and anthocyanins were shown to be distinctive when compared to grape wines. Naringenin and apigenin were characteristic only for cherry wine, and seven anthocyanins were distinctive for cherry wine.

  16. Detailed insight into hordeins expression in high protein cultivars as a first stage to improve feed quality

    DEFF Research Database (Denmark)

    Kaczmarczyk, Agnieszka Ewa; Aaslo, Per; Vincze, Éva

    2011-01-01

    in all samples were analyzed using UPLC method. It was noticed that there are big differences in quantity and quality of storage proteins transcripts in consecutive developmental phases and among selected cultivars. The data about mRNA expression, amino acids and total protein concentrations were plotted...... novel cereal cultivars with high protein concentration and better amino acid composition. Six barley cultivars with high protein content (Netto, PR3440, Kontiki, Fairytale, PR3528 and IC364) and Golden Promise as a control were selected for analysis of hordeins expression at four stages of grain...... development. All the known hordein coding sequences collected from commonly available databases (NCBI, HarvEST) were classified into groups and subgroups (B1, B3, C, D and Γ). Specific primers were designed with Primique and AlleleID softwares and checked with qRT-PCR first on the control cultivar Golden...

  17. A pollen-expressed gene for a novel protein with an F-box motif that is very tightly linked to a gene for S-RNase in two species of cherry, Prunus cerasus and P. avium.

    Science.gov (United States)

    Yamane, Hisayo; Ikeda, Kazuo; Ushijima, Koichiro; Sassa, Hidenori; Tao, Ryutaro

    2003-07-01

    This study describes a novel F-box protein gene in the S-locus of sour cherry (Prunus cerasus) and sweet cherry (P. avium). The gene showed an S-haplotype-specific sequence polymorphism and the expression was specific to pollen. Genomic DNA blot analysis of eight sweet cherry cultivars with the probe for the F-box protein gene under low stringency conditions yielded RFLP bands specific to the S-haplotypes of each cultivar. We discuss the possibility of the gene for the F-box protein being a candidate for the male determinant of gametophytic self-incompatibility in PRUNUS:

  18. Allele-specific PCR detection of sweet cherry self-incompatibility (S) alleles S1 to S16 using consensus and allele-specific primers.

    Science.gov (United States)

    Sonneveld, T; Tobutt, K R; Robbins, T P

    2003-10-01

    PCR-based identification of all 13 known self-incompatibility (S) alleles of sweet cherry is reported. Two pairs of consensus primers were designed from our previously published cDNA sequences of S(1) to S(6) S-RNases, the stylar components of self-incompatibility, to reveal length variation of the first and the second introns. With the exception of the first intron of S(13), these also amplified S(7) to S(14) and an allele previously referred to as S(x), which we now label S(16). The genomic PCR products were cloned and sequenced. The partial sequence of S(11) matched that of S(7) and the alleles were shown to have the same functional specificity. Allele-specific primers were designed for S(7) to S(16), so that allele-specific primers are now available for all 13 S alleles of cherry (S(8), S(11) and S(15) are duplicates). These can be used to distinguish between S alleles with introns of similar size and to confirm genotypes determined with consensus primers. The reliability of the PCR with allele-specific primers was improved by the inclusion of an internal control. The use of the consensus and allele-specific primers was demonstrated by resolving conflicting genotypes that have been published recently and by determining genotypes of 18 new cherry cultivars. Two new groups are proposed, Group XXIII (S(3) S(16)), comprising 'Rodmersham Seedling' and 'Strawberry Heart', and Group XXIV (S(6) S(12)), comprising 'Aida' and 'Flamentiner'. Four new self-compatibility genotypes, S(3) S(3)', S(4)' S(6), S(4)' S(9) and S(4)' S(13), were found. The potential use of the consensus primers to reveal incompatibility alleles in other cherry species is also demonstrated.

  19. External potassium (K(+)) application improves salinity tolerance by promoting Na(+)-exclusion, K(+)-accumulation and osmotic adjustment in contrasting peanut cultivars.

    Science.gov (United States)

    Chakraborty, Koushik; Bhaduri, Debarati; Meena, Har Narayan; Kalariya, Kuldeepsingh

    2016-06-01

    Achieving salt-tolerance is highly desirable in today's agricultural context. Apart from developing salt-tolerant cultivars, possibility lies with management options, which can improve crop yield and have significant impact on crop physiology as well. Thus present study was aimed to evaluate the ameliorative role of potassium (K(+)) in salinity tolerance of peanut. A field experiment was conducted using two differentially salt-responsive cultivars and three levels of salinity treatment (control, 2.0 dS m(-1), 4.0 dS m(-1)) along with two levels (with and without) of potassium fertilizer (0 and 30 kg K2O ha(-1)). Salinity treatment incurred significant changes in overall physiology in two peanut cultivars, though the responses varied between the tolerant and the susceptible one. External K(+) application resulted in improved salinity tolerance in terms of plant water status, biomass produced under stress, osmotic adjustment and better ionic balance. Tolerant cv. GG 2 showed better salt tolerance by excluding Na(+) from uptake and lesser accumulation in leaf tissue and relied more on organic osmolyte for osmotic adjustment. On the contrary, susceptible cv. TG 37A allowed more Na(+) to accumulate in the leaf tissue and relied more on inorganic solute for osmotic adjustment under saline condition, hence showed more susceptibility to salinity stress. Application of K(+) resulted in nullifying the negative effect of salinity stress with slightly better response in the susceptible cultivar (TG 37A). The present study identified Na(+)-exclusion as a key strategy for salt-tolerance in tolerant cv. GG 2 and also showed the ameliorating role of K(+) in salt-tolerance with varying degree of response amongst tolerant and susceptible cultivars.

  20. Maraschino Cherry: A Laboratory-Lecture Unit

    Science.gov (United States)

    Wrolstad, Ronald E.

    2009-01-01

    Oregon State Univ. has offered FST 102 "Maraschino Cherry" as a 1-credit orientation course since 1994. The maraschino cherry serves as a vehicle from which faculty give their disciplinary perspective, for example, the chemistry of the maraschino cherry, processing unit operations, microbiology and food safety, food law, sensory…

  1. 21 CFR 145.125 - Canned cherries.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Canned cherries. 145.125 Section 145.125 Food and... CONSUMPTION CANNED FRUITS Requirements for Specific Standardized Canned Fruits § 145.125 Canned cherries. (a) Identity—(1) Ingredients. Canned cherries is the food prepared from one of the optional fresh or...

  2. Maraschino Cherry: A Laboratory-Lecture Unit

    Science.gov (United States)

    Wrolstad, Ronald E.

    2009-01-01

    Oregon State Univ. has offered FST 102 "Maraschino Cherry" as a 1-credit orientation course since 1994. The maraschino cherry serves as a vehicle from which faculty give their disciplinary perspective, for example, the chemistry of the maraschino cherry, processing unit operations, microbiology and food safety, food law, sensory analysis, product…

  3. Volatile Compound Profiling by HS-SPME/GC-MS-FID of a Core Olive Cultivar Collection as a Tool for Aroma Improvement of Virgin Olive Oil

    Directory of Open Access Journals (Sweden)

    Lourdes García-Vico

    2017-01-01

    Full Text Available Virgin olive oil (VOO is the only food product requiring official sensory analysis to be classified in commercial categories, in which the evaluation of the aroma plays a very important role. The selection of parents, with the aim of obtaining new cultivars with improved oil aroma, is of paramount importance in olive breeding programs. We have assessed the volatile fraction by headspace-solid-phase microextraction/gas chromatography-mass spectrometry-flame ionization detection (HS-SPME/GC-MS-FID and the deduced aroma properties of VOO from a core set of olive cultivars (Core-36 which possesses most of the genetic diversity found in the World Olive Germplasm Collection (IFAPA Alameda del Obispo located in Cordoba, Spain. The VOO volatile fractions of Core-36 cultivars display a high level of variability. It is mostly made of compounds produced from polyunsaturated fatty acids through the lipoxygenase pathway, which confirms to be a general characteristic of the olive species (Olea europaea L.. The main group of volatile compounds in the oils was six straight-chain carbon compounds derived from linolenic acid, some of them being the main contributors to the aroma of the olive oils according to their odor activity values (OAV. The high level of variability found for the volatile fraction of the oils from Core-36 and, therefore, for the aroma odor notes, suggest that this core set may be a very useful tool for the choice of optimal parents in olive breeding programs in order to raise new cultivars with improved VOO aroma.

  4. Volatile Compound Profiling by HS-SPME/GC-MS-FID of a Core Olive Cultivar Collection as a Tool for Aroma Improvement of Virgin Olive Oil.

    Science.gov (United States)

    García-Vico, Lourdes; Belaj, Angjelina; Sánchez-Ortiz, Araceli; Martínez-Rivas, José M; Pérez, Ana G; Sanz, Carlos

    2017-01-14

    Virgin olive oil (VOO) is the only food product requiring official sensory analysis to be classified in commercial categories, in which the evaluation of the aroma plays a very important role. The selection of parents, with the aim of obtaining new cultivars with improved oil aroma, is of paramount importance in olive breeding programs. We have assessed the volatile fraction by headspace-solid-phase microextraction/gas chromatography-mass spectrometry-flame ionization detection (HS-SPME/GC-MS-FID) and the deduced aroma properties of VOO from a core set of olive cultivars (Core-36) which possesses most of the genetic diversity found in the World Olive Germplasm Collection (IFAPA Alameda del Obispo) located in Cordoba, Spain. The VOO volatile fractions of Core-36 cultivars display a high level of variability. It is mostly made of compounds produced from polyunsaturated fatty acids through the lipoxygenase pathway, which confirms to be a general characteristic of the olive species (Olea europaea L.). The main group of volatile compounds in the oils was six straight-chain carbon compounds derived from linolenic acid, some of them being the main contributors to the aroma of the olive oils according to their odor activity values (OAV). The high level of variability found for the volatile fraction of the oils from Core-36 and, therefore, for the aroma odor notes, suggest that this core set may be a very useful tool for the choice of optimal parents in olive breeding programs in order to raise new cultivars with improved VOO aroma.

  5. Genetic Diversity Assessment and Identification of New Sour Cherry Genotypes Using Intersimple Sequence Repeat Markers

    OpenAIRE

    Roghayeh Najafzadeh; Kazem Arzani; Naser Bouzari; Ali Saei

    2014-01-01

    Iran is one of the chief origins of subgenus Cerasus germplasm. In this study, the genetic variation of new Iranian sour cherries (which had such superior growth characteristics and fruit quality as to be considered for the introduction of new cultivars) was investigated and identified using 23 intersimple sequence repeat (ISSR) markers. Results indicated a high level of polymorphism of the genotypes based on these markers. According to these results, primers tested in this study specially IS...

  6. The ground cherry(cerasus fruticosa Pall).a new kind of edible cherry for cold and dry regions of China

    Institute of Scientific and Technical Information of China (English)

    DingXiaodong; SuiWei; 等

    1994-01-01

    We have conducted some studies on 3 ground cherry cultivars' artaskaja','Bushkaja' and Maksmorfikaja' introduced from Russia,The results indicated that these cultivars could survive temperature-40℃ during severe winter in Harbin,China and had high drought and disease resistance,the average weight of each fruit reached 2.78g,the contents of Vc and soluble solid exceeded 30mg/100g and 12%,respectively.Not only does the fruits suit for fresh market,but also for processing.Ground cherry is a promising new fruit crop in cold region.

  7. Free and glycosidically bound aroma compounds in cherry (Prunus avium L.).

    Science.gov (United States)

    Wen, Ya-Qin; He, Fei; Zhu, Bao-Qing; Lan, Yi-Bin; Pan, Qiu-Hong; Li, Chun-You; Reeves, Malcolm J; Wang, Jun

    2014-01-01

    This paper reports the occurrence of both free and glycosidically bound aroma compounds in three sweet cherry cultivars ('Hongdeng', 'Hongyan' and 'Rainier'), with 97 compounds being identified in the three cultivars. The major free volatile compounds found were hexanal, (E)-2-hexenal, (E)-2-hexen-1-ol, benzyl alcohol and benzaldehyde. The major bound volatile compounds found were benzyl alcohol, geraniol, 2-phenylethanol. Also 4-vinylphenol was found in cherry fruit for the first time, and has a relatively high concentration of the glycosidically-bound form in 'Rainier'. Odour activity values (OAVs) were determined for both free and bound volatiles, with 18 compounds having an OAV above 1. The highest OAVs for three cultivars were (E)-β-ionone, hexanal, decanal and (E)-2-hexenal with the highest being over 800 for (E)-β-ionone in 'Honyang'. From these results, it was concluded that the aroma compounds present were similar in all three cultivars, but there was significant variation found in their levels and hence contribution to the aroma of these cultivars.

  8. Color Parameters and Total Anthocyanins of Sour Cherries (Prunus cerasus L. During Ripening

    Directory of Open Access Journals (Sweden)

    Sandra Pedišić

    2009-12-01

    Full Text Available Color is very important indicator of quality of fresh fruit. It also serves for estimating the stage of maturity of fruit. Plant pigments responsible for the color of some kind of fruits are anthocyanins. Anthocyanins are the flavonoids which are present in high amounts in sour cherries. The aim of this study was to determine total anthocyanins and color parameters of sour cherries ‘Cigančica’ and ‘Keleris’ collected in Osijek and Zadar (Croatia in 2005 during ripening. Color parameters of skin and fl esh of sour cherries were determined with colorimetric CIE LAB method and total anthocyanins were determined by means of high performance liquid chromatography (HPLC using UV/VIS PDA detector. Total anthocyanin was higher in sour cherries cv. Keleris grown in Zadar than in cv. Cigančica grown in Osijek during ripening although cv. Keleris is light colored genotype. Obtained results suggested that warm Mediterranean climate could have influence on high anthocyanin synthesis during ripening. Analysis of variance showed that stage of ripening did not influence total anthocyanin concentrations, but influenced almost all color parameters. Parameter H° was good indicator of color variation during ripening in both sour cherry cultivars.

  9. Ireland's Cherry Orchard National School

    Science.gov (United States)

    O Cuiv, Shan

    2007-01-01

    This recently completed primary school illustrates how architecture can contribute to creating a safe and warm environment in a difficult area and can meet the particular needs of the student community. In its first year in operation, Cherry Orchard National School is proving to be a successful project. Presented here are the architectural…

  10. Chopping Down the Cherry Tree.

    Science.gov (United States)

    Griswold, Jerry

    1995-01-01

    Attempts once again to put to rest the infamous "I cannot tell a lie" episode involving George Washington and a downed cherry tree. Appends an editor's note that states that William Bennett's "The Children's Book of Virtues" which perpetuates this infamous piece of "fakelore." (RS)

  11. Measuring the expression of hordeins in high protein cultivars as a first step to improve feed quality

    DEFF Research Database (Denmark)

    Kaczmarczyk, Agnieszka Ewa; Aaslo, Per; Elek, Zoltán

    2011-01-01

    , C, D and Γ). Specific primers were designed with Primique and AlleleID software and checked with qRT-PCR first on the Golden Promise followed by the gene expression analysis of the collected material from the high protein cultivars. According to the standard curve prepared for actin...... exploiting as a feed. Hordeins, the main storage proteins of barley grain, have a less optimal amino acid profile characterized by a low content or lack of essential amino acids (lysine, cysteine) and a high content of non-essential amino acids (proline, glutamine). Hordeins consist of gene families...... transcripts in consecutive developmental phases and among different cultivars. The amount of mRNA was compared with amino acid profiles and protein concentrations to obtain correlation among these phenomena....

  12. Tart Cherry Extracts Reduce Inflammatory and Oxidative Stress Signaling in Microglial Cells.

    Science.gov (United States)

    Shukitt-Hale, Barbara; Kelly, Megan E; Bielinski, Donna F; Fisher, Derek R

    2016-09-22

    Tart cherries contain an array of polyphenols that can decrease inflammation and oxidative stress (OS), which contribute to cognitive declines seen in aging populations. Previous studies have shown that polyphenols from dark-colored fruits can reduce stress-mediated signaling in BV-2 mouse microglial cells, leading to decreases in nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression. Thus, the present study sought to determine if tart cherries-which improved cognitive behavior in aged rats-would be efficacious in reducing inflammatory and OS signaling in HAPI rat microglial cells. Cells were pretreated with different concentrations (0-1.0 mg/mL) of Montmorency tart cherry powder for 1-4 h, then treated with 0 or 100 ng/mL lipopolysaccharide (LPS) overnight. LPS application increased extracellular levels of NO and tumor necrosis factor-alpha (TNF-α), and intracellular levels of iNOS and cyclooxygenase-2 (COX-2). Pretreatment with tart cherry decreased levels of NO, TNF-α, and COX-2 in a dose- and time-dependent manner versus those without pretreatment; the optimal combination was between 0.125 and 0.25 mg/mL tart cherry for 2 h. Higher concentrations of tart cherry powder and longer exposure times negatively affected cell viability. Therefore, tart cherries (like other dark-colored fruits), may be effective in reducing inflammatory and OS-mediated signals.

  13. Graus de resistência à brusone e produtividade de cultivares melhoradas de arroz de terras altas Degree of blast resistance and grain yield of improved upland rice cultivars

    Directory of Open Access Journals (Sweden)

    Anne Sitarama Prabhu

    2001-12-01

    Full Text Available O objetivo deste trabalho foi estudar o nível de resistência à brusone nas folhas e panículas e seu efeito sobre a produtividade de 12 cultivares de arroz, em experimento de campo, durante três anos consecutivos. O grau de resistência das cultivares à brusone nas folhas e panículas foi determinado utilizando-se o critério de área sob curva de progresso e severidade de brusone nas panículas dez dias antes de colheita, respectivamente. As cultivares Progresso, Cuiabana, Caiapó, Carajás e Araguaia, em ordem decrescente, foram as mais resistentes à brusone nas folhas. As cultivares Carajás e Progresso apresentaram severidades da brusone, nas panículas, menores do que as demais cultivares em dois anos. A produtividade das cultivares nos três anos de avaliações, que variou de 83 kg/ha na IAC 201 a 3.617 kg/ha na Rio Paraguai, é explicada principalmente pela severidade da brusone nas panículas, já que as correlações foram negativas e significativas. As raças de Pyricularia grisea, IB-9, IB-41, IB-13 e IC-27, provenientes de 13 cultivares, apresentaram interações diferenciais. Os resultados permitiram concluir que o grau de resistência de algumas cultivares é inadequado e necessita de outras medidas de controle.The aim of this work was to study the levels of leaf and panicle blast resistance and their effect on grain yield of 12 rice cultivars, in a field experiment, during consecutive three years. The degree of leaf blast and panicle blast resistance was determined by the assessment criteria of area under disease progress curve and disease severity ten days before the harvest, respectively. The cultivars Progresso, Cuiabana, Caiapó, Carajás and Araguaia were superior in descending order for leaf blast resistance. For panicle blast, Carajás and Progresso, exhibited lower disease severities than the rest of the cultivars in the first two years. The grain yields during three years ranged from 83 kg/ha for IAC 201 to 3

  14. Characterization of cornelian cherry (Cornus mas L. genotypes - genetic resources for food production in Czech Republic

    Directory of Open Access Journals (Sweden)

    Sochor Jiri

    2014-01-01

    Full Text Available The aim of the study was to determine and compare both technological properties and polyphenol content in fruits of eight cornellian cherry (Cornus mas L. cultivars from Czech Republic. The fruits of cultivar ´Vydubeckij´ had the highest dry matter (17.4% and soluble solid (15.8% content among searched cultivars. The highest crude protein and phosphorus values were determined in the fruits of the ´Titus´ cultivar as 10.9 g of crude protein and 435 mg of phosphorus for per kg fresh weight (FW base. The ´Elegantnyj´ and ´Vydubeckij´ cultivars are found to be more suitable for canning industry. The fruits of the ´Vydubeckij´ cultivar contained 10.8 g of pectin and 22.4 g of citric acid per kg fresh weight base, and could be important for technological use because of their excellent gelling ability. ´Devin´,´Vydubeckij´ and ´Titus´ cultivars had the most valuable source of chlorogenic acid (135.6, 110.9 and 115.1 mg per 100 g FW, respectively and quercetin (24.9, 25.2 and 24.2 mg per 100 g FW, respectively. This work should contribute to the popularization of this fruit specie as a valuable source of technological parameters for human nutrition and the canning industry.

  15. Michigan Tart Cherry Processors: Issues and Strategy

    OpenAIRE

    Martinez, Lourdes R.; Thornsbury, Suzanne

    2006-01-01

    Michigan accounts for approximately 70 percent of the total number of tart cherry processing firms in the U.S. Changes in preferences of consumers and increasing participation of global competitors are driving down demand for traditional tart cherry products and imposing new pressures on the U.S. industry, particularly in Michigan. The objective of this report is to document main characteristics of the tart cherry processing industry in Michigan; namely, business characteristics, category of ...

  16. Exogenous Cytokinins Increase Grain Yield of Winter Wheat Cultivars by Improving Stay-Green Characteristics under Heat Stress.

    Directory of Open Access Journals (Sweden)

    Dongqing Yang

    Full Text Available Stay-green, a key trait of wheat, can not only increase the yield of wheat but also its resistance to heat stress during active photosynthesis. Cytokinins are the most potent general coordinator between the stay-green trait and senescence. The objectives of the present study were to identify and assess the effects of cytokinins on the photosynthetic organ and heat resistance in wheat. Two winter wheat cultivars, Wennong 6 (a stay-green cultivar and Jimai 20 (a control cultivar, were subjected to heat stress treatment from 1 to 5 days after anthesis (DAA. The two cultivars were sprayed daily with 10 mg L-1 of 6-benzylaminopurine (6-BA between 1 and 3 DAA under ambient and elevated temperature conditions. We found that the heat stress significantly decreased the number of kernels per spike and the grain yield (P < 0.05. Heat stress also decreased the zeatin riboside (ZR content, but increased the gibberellin (GA3, indole-3-acetic acid (IAA, and abscisic acid (ABA contents at 3 to 15 DAA. Application of 6-BA significantly (P < 0.05 increased the grain-filling rate, endosperm cell division rate, endosperm cell number, and 1,000-grain weight under heated condition. 6-BA application increased ZR and IAA contents at 3 to 28 DAA, but decreased GA3 and ABA contents. The contents of ZR, ABA, and IAA in kernels were positively and significantly correlated with the grain-filling rate (P < 0.05, whereas GA3 was counter-productive at 3 to 15 DAA. These results suggest that the decrease in grain yield under heat stress was due to a lower ZR content and a higher GA3 content compared to that at elevated temperature during the early development of the kernels, which resulted in less kernel number and lower grain-filling rate. The results also provide essential information for further utilization of the cytokinin substances in the cultivation of heat-resistant wheat.

  17. Open cherry picker simulation results

    Science.gov (United States)

    Nathan, C. A.

    1982-01-01

    The simulation program associated with a key piece of support equipment to be used to service satellites directly from the Shuttle is assessed. The Open Cherry Picker (OCP) is a manned platform mounted at the end of the remote manipulator system (RMS) and is used to enhance extra vehicular activities (EVA). The results of simulations performed on the Grumman Large Amplitude Space Simulator (LASS) and at the JSC Water Immersion Facility are summarized.

  18. Procyanidins in fruit from Sour cherry (Prunus cerasus) differ strongly in chainlength from those in Laurel cherry (Prunus lauracerasus) and Cornelian cherry (Cornus mas)

    NARCIS (Netherlands)

    Capanoglu, E.; Boyacioglu, D.; Vos, de R.C.H.; Hall, R.D.; Beekwilder, M.J.

    2011-01-01

    Sour cherry (Prunus cerasus), Laurel cherry (Prunus lauracerasus), and Cornelian cherry (Cornus mas) fruits are widely used in Turkey, both as food and as traditional medicines. The phytochemical composition and antioxidant capacities of these three cherry types were compared. Fruit flesh was evalua

  19. Improved tolerance to post-anthesis drought stress by pre-drought priming at vegetative stages in drought-tolerant and -sensitive wheat cultivars.

    Science.gov (United States)

    Abid, Muhammad; Tian, Zhongwei; Ata-Ul-Karim, Syed Tahir; Liu, Yang; Cui, Yakun; Zahoor, Rizwan; Jiang, Dong; Dai, Tingbo

    2016-09-01

    Wheat crop endures a considerable penalty of yield reduction to escape the drought events during post-anthesis period. Drought priming under a pre-drought stress can enhance the crop potential to tolerate the subsequent drought stress by triggering a faster and stronger defense mechanism. Towards these understandings, a set of controlled moderate drought stress at 55-60% field capacity (FC) was developed to prime the plants of two wheat cultivars namely Luhan-7 (drought tolerant) and Yangmai-16 (drought sensitive) during tillering (Feekes 2 stage) and jointing (Feekes 6 stage), respectively. The comparative response of primed and non-primed plants, cultivars and priming stages was evaluated by applying a subsequent severe drought stress at 7 days after anthesis. The results showed that primed plants of both cultivars showed higher potential to tolerate the post-anthesis drought stress through improved leaf water potential, more chlorophyll, and ribulose-1, 5-bisphosphate carboxylase/oxygenase contents, enhanced photosynthesis, better photoprotection and efficient enzymatic antioxidant system leading to less yield reductions. The primed plants of Luhan-7 showed higher capability to adapt the drought stress events than Yangmai-16. The positive effects of drought priming to sustain higher grain yield were pronounced in plants primed at tillering than those primed at jointing. In consequence, upregulated functioning of photosynthetic apparatus and efficient enzymatic antioxidant activities in primed plants indicated their superior potential to alleviate a subsequently occurring drought stress, which contributed to lower yield reductions than non-primed plants. However, genotypic and priming stages differences in response to drought stress also contributed to affect the capability of primed plants to tolerate the post-anthesis drought stress conditions in wheat.

  20. Micropropagation of Prunus species relevant to cherry fruit production.

    Science.gov (United States)

    Druart, Philippe

    2013-01-01

    Cherry tree micropropagation is limited to the production of healthy cultivars of Prunus avium and Prunus cerasus, and their rootstocks; mainly the dwarfing ones. By using meristem-tip (0.1 mm long) or healthy shoot tips/nodes, four successive steps are needed to obtain whole plants capable of growing in the nursery: multiplication by axillary branching, shoot elongation, rooting, and plantlet acclimation. Along this process, several parameters have to be adjusted for each phase of the culture, including media composition, environmental culture conditions and plant handling. These parameters vary depending on genotypic response and specific vulnerability to physiological disorders such as hyperhydricity, apex necrosis, unstable propagation, and rooting rates. Based on a 40 year-long experience of study and application of culture conditions to large-scale plant production, this document summarizes the main problems (variability of the propagation rate, hyperhydricity, apex necrosis, plant re-growth) and solutions encountered to solve them, with means validated on many mericlones.

  1. Effects of alginate edible coating on quality and antioxidant properties in sweet cherry during postharvest storage

    Directory of Open Access Journals (Sweden)

    V. Chiabrando

    2015-06-01

    Full Text Available Two sweet cherry (Prunus avium L. cultivars (“Big Lory” and “Grace Star”, were treated with 1%, 3% and 5% sodium alginate as an edible coating before storage. Analytical determinations were made after 7, 14 and 21 days of storage at 4°C. Cherries were analyzed for the following quality parameters: firmness, weight loss, titratable acidity, soluble solid content, external color, anthocyanin content, phenolic content and total antioxidant capacity. Alginate treatment at 3% delayed changes in most of the ripening parameters, weight and acidity losses, softening and color changes. However, the soluble solids content was not affected by the alginate fruit coating. In terms of the antioxidant properties, no significant results were obtained with the use of the alginate coating. The results of this study suggest that alginate treatments at 1% and 3% could be used as natural postharvest treatments in cherry cultivars with the aim of delaying the postharvest ripening process and maintaining fruit quality.

  2. Sour Cherry (Prunus cerasus L Anthocyanins as Ingredients for Functional Foods

    Directory of Open Access Journals (Sweden)

    Federica Blando

    2004-01-01

    Full Text Available In the recent years many studies on anthocyanins have revealed their strong antioxidant activity and their possible use as chemotherapeutics. The finding that sour cherries (Prunus cerasus L (also called tart cherries contain high levels of anthocyanins that possess strong antioxidant and anti-inflammatory properties has attracted much attention to this species. Here we report the preliminary results of the induction of anthocyanin biosynthesis in sour cherry callus cell cultures. The evaluation and characterization of the in vitro produced pigments are compared to those of the anthocyanins found in vivo in fruits of several sour cherry cultivars. Interestingly, the anthocyanin profiles found in whole fruit extracts were similar in all tested genotypes but were different with respect to the callus extract. The evaluation of antioxidant activity, performed by ORAC and TEAC assays, revealed a relatively high antioxidant capacity for the fruit extracts (from 1145 to 2592 μmol TE/100 g FW and a lower one for the callus extract (688 μmol TE/100 g FW.

  3. Pulsed electric field improves the bioprotective capacity of purées for different coloured carrot cultivars against H2O2-induced oxidative damage.

    Science.gov (United States)

    Leong, Sze Ying; Oey, Indrawati; Burritt, David John

    2016-04-01

    This research aimed to study the effect of pulsed electric field (PEF) processing on the bioprotective capacity of carrot purée for White Belgian, Yellow Solar, Nantes, Nutri Red and Purple Haze cultivars against H2O2-induced oxidative damage. The bioprotective capacity was determined using cell viability, membrane integrity and nitric oxide (NO) production in a human Caco-2 cell culture assay. Total carotenoids, total anthocyanins, total vitamin C and total phenolics were also evaluated. Compared to the untreated purée, Purple Haze and Nutri Red processed at 303 kJ/kg completely increased Caco-2 cells resistance towards oxidative damage by recovering the cell viability and inhibiting NO production. For cultivar with low carotenoid levels, i.e. Yellow Solar, the application of 0.8 kV/cm resulted in a higher total carotenoid content in the purée than its untreated counterpart, leading to an improved bioprotective effect. This study clearly shows that PEF could add value to carrots by maximising bioprotective effects.

  4. Speed, Acceleration, Chameleons and Cherry Pit Projectiles

    Science.gov (United States)

    Planinsic, Gorazd; Likar, Andrej

    2012-01-01

    The paper describes the mechanics of cherry pit projectiles and ends with showing the similarity between cherry pit launching and chameleon tongue projecting mechanisms. The whole story is written as an investigation, following steps that resemble those typically taken by scientists and can therefore serve as an illustration of scientific…

  5. Characterization of microsatellites in wild and sweet cherry (Prunus avium L.)--markers for individual identification and reproductive processes.

    Science.gov (United States)

    Schueler, Silvio; Tusch, Alexandra; Schuster, Mirko; Ziegenhagen, Birgit

    2003-02-01

    Nuclear microsatellites were characterized in Prunus avium and validated as markers for individual and cultivar identification, as well as for studies of pollen- and seed-mediated gene flow. We used 20 primer pairs from a simple sequence repeat (SSR) library of Prunus persica and identified 7 loci harboring polymorphic microsatellite sequences in P. avium. In a natural population of 75 wild cherry trees, the number of alleles per locus ranged from 4 to 9 and expected heterozygosity from 0.39 to 0.77. The variability of the SSR markers allowed an unambiguous identification of individual trees and potential root suckers. Additionally, we analyzed 13 sweet cherry cultivars and differentiated 12 of them. An exclusion probability of 0.984 was calculated, which indicates that the seven loci are suitable markers for paternity analysis. The woody endocarp was successfully used for resolution of all microsatellite loci and exhibited the same multilocus genotype as the mother tree, as shown in a single seed progeny. Hence, SSR fingerprinting of the purely maternal endocarp was also successful in this Prunus species, allowing the identification of the mother tree of the dispersed seeds. The linkage of microsatellite loci with PCR-amplified alleles of the self-incompatibility locus was tested in two full-sib families of sweet cherry cultivars. From low recombination frequencies, we inferred that two loci are linked with the S locus. The present study provides markers that will significantly facilitate studies of spatial genetic variation and gene flow in wild cherry, as well as breeding programs in sweet cherry.

  6. 7 CFR 930.15 - Restricted percentage cherries.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Restricted percentage cherries. 930.15 Section 930.15... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES... Handling Definitions § 930.15 Restricted percentage cherries. Restricted percentage cherries means...

  7. 21 CFR 145.126 - Artificially sweetened canned cherries.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Artificially sweetened canned cherries. 145.126... § 145.126 Artificially sweetened canned cherries. (a) Artificially sweetened canned cherries is the food which conforms to the definition and standard of identity prescribed for canned cherries by §...

  8. Monitoring Drosophila suzukii Matsumura in Oregon, USA sweet cherry orchards.

    Science.gov (United States)

    Drosophila suzukii rapidly became a significant cherry pest in the western United States after it was observed damaging cherries in 2009 in California. It has caused significant damage to ripening cherries in all major USA cherry production districts leading to increased management costs and reduced...

  9. Characterization of cuticle composition after cold storage of "Celeste" and "Somerset" sweet cherry fruit.

    Science.gov (United States)

    Belge, Burcu; Llovera, Montserrat; Comabella, Eva; Gatius, Ferran; Guillén, Pere; Graell, Jordi; Lara, Isabel

    2014-08-27

    Cuticle composition and structure may be relevant factors affecting the storage potential of fruits, but very few studies have analyzed fruit cuticle composition from a postharvest perspective. In this work, the chemical composition of waxes and cutin (major cuticular components) was analyzed in cuticle samples isolated from "Celeste" and "Somerset" cherries (Prunus avium L.) after cold storage at 0 °C. Total cuticle amounts per surface unit (μg cm(-2)) increased along with cold storage. The triterpene ursolic acid, the alkane nonacosane, linoleic acid, and β-sitosterol were the most abundant components of cuticular waxes, whereas cutin composition was dominated by C18-type monomers. In spite of being comprised of similar chemical families, cultivar-related differences were found regarding the abundance and the evolution of some compound families during cold storage. To the best of our knowledge, this is the first report on changes in cuticle composition of sweet cherry during postharvest storage.

  10. Tart Cherry Extracts Reduce Inflammatory and Oxidative Stress Signaling in Microglial Cells

    Directory of Open Access Journals (Sweden)

    Barbara Shukitt-Hale

    2016-09-01

    Full Text Available Tart cherries contain an array of polyphenols that can decrease inflammation and oxidative stress (OS, which contribute to cognitive declines seen in aging populations. Previous studies have shown that polyphenols from dark-colored fruits can reduce stress-mediated signaling in BV-2 mouse microglial cells, leading to decreases in nitric oxide (NO production and inducible nitric oxide synthase (iNOS expression. Thus, the present study sought to determine if tart cherries—which improved cognitive behavior in aged rats—would be efficacious in reducing inflammatory and OS signaling in HAPI rat microglial cells. Cells were pretreated with different concentrations (0–1.0 mg/mL of Montmorency tart cherry powder for 1–4 h, then treated with 0 or 100 ng/mL lipopolysaccharide (LPS overnight. LPS application increased extracellular levels of NO and tumor necrosis factor-alpha (TNF-α, and intracellular levels of iNOS and cyclooxygenase-2 (COX-2. Pretreatment with tart cherry decreased levels of NO, TNF-α, and COX-2 in a dose- and time-dependent manner versus those without pretreatment; the optimal combination was between 0.125 and 0.25 mg/mL tart cherry for 2 h. Higher concentrations of tart cherry powder and longer exposure times negatively affected cell viability. Therefore, tart cherries (like other dark-colored fruits, may be effective in reducing inflammatory and OS-mediated signals.

  11. Improved Agrobacterium-mediated transformation and high efficiency of root formation from hypocotyl meristem of spring Brassica napus 'Precocity' cultivar.

    Science.gov (United States)

    Liu, X X; Lang, S R; Su, L Q; Liu, X; Wang, X F

    2015-12-14

    Rape seed (Brassica napus L.) is one of the most important oil seed crops in the world. Genetic manipulation of rapeseed requires a suitable tissue culture system and an efficient method for plant regeneration, as well as an efficient transformation procedure. However, development of transgenic B. napus has been problematic, and current studies are limited to cultivated varieties. In this study, we report a protocol for regeneration of transgenic rape after Agrobacterium-mediated transformation of hypocotyls from the spring B. napus 'Precocity' cultivar. We analyzed the effects of plant growth regulators in the medium on regeneration. Additionally, factors affecting the transformation efficiency, including seedling age, Agrobacterium concentration, infection time, and co-cultivation time, were assessed by monitoring GUS expression. Results from these experiments revealed that transformation was optimized when the meristematic parts of the hypocotyls were taken from 8 day-old seedlings, cultured on Murashinge and Skoog basal media containing 0.1 mg/L 1-naphthaleneacetic acid and 2.5 mg/L 6-benzylaminopurine, and incubated in Agrobacterium suspension (OD600 = 0.5) for 3 to 5 min, followed by 2 days of co-cultivation. Integration of T-DNA into the plant genome was confirmed by polymerase chain reaction (PCR), b-glucuronidase histochemical staining, and quantitative real-time PCR. The protocols developed for regeneration, transformation, and rooting described in this study could help to accelerate the development of transgenic spring rape varieties with novel features.

  12. Inheritance of hetero-diploid pollen S-haplotype in self-compatible tetraploid Chinese cherry (Prunus pseudocerasus Lindl).

    Science.gov (United States)

    Gu, Chao; Liu, Qing-Zhong; Yang, Ya-Nan; Zhang, Shu-Jun; Khan, Muhammad Awais; Wu, Jun; Zhang, Shao-Ling

    2013-01-01

    The breakdown of self-incompatibility, which could result from the accumulation of non-functional S-haplotypes or competitive interaction between two different functional S-haplotypes, has been studied extensively at the molecular level in tetraploid Rosaceae species. In this study, two tetraploid Chinese cherry (Prunus pseudocerasus) cultivars and one diploid sweet cherry (Prunus avium) cultivar were used to investigate the ploidy of pollen grains and inheritance of pollen-S alleles. Genetic analysis of the S-genotypes of two intercross-pollinated progenies showed that the pollen grains derived from Chinese cherry cultivars were hetero-diploid, and that the two S-haplotypes were made up of every combination of two of the four possible S-haplotypes. Moreover, the distributions of single S-haplotypes expressed in self- and intercross-pollinated progenies were in disequilibrium. The number of individuals of the two different S-haplotypes was unequal in two self-pollinated and two intercross-pollinated progenies. Notably, the number of individuals containing two different S-haplotypes (S1- and S5-, S5- and S8-, S1- and S4-haplotype) was larger than that of other individuals in the two self-pollinated progenies, indicating that some of these hetero-diploid pollen grains may have the capability to inactivate stylar S-RNase inside the pollen tube and grow better into the ovaries.

  13. Inheritance of hetero-diploid pollen S-haplotype in self-compatible tetraploid Chinese cherry (Prunus pseudocerasus Lindl.

    Directory of Open Access Journals (Sweden)

    Chao Gu

    Full Text Available The breakdown of self-incompatibility, which could result from the accumulation of non-functional S-haplotypes or competitive interaction between two different functional S-haplotypes, has been studied extensively at the molecular level in tetraploid Rosaceae species. In this study, two tetraploid Chinese cherry (Prunus pseudocerasus cultivars and one diploid sweet cherry (Prunus avium cultivar were used to investigate the ploidy of pollen grains and inheritance of pollen-S alleles. Genetic analysis of the S-genotypes of two intercross-pollinated progenies showed that the pollen grains derived from Chinese cherry cultivars were hetero-diploid, and that the two S-haplotypes were made up of every combination of two of the four possible S-haplotypes. Moreover, the distributions of single S-haplotypes expressed in self- and intercross-pollinated progenies were in disequilibrium. The number of individuals of the two different S-haplotypes was unequal in two self-pollinated and two intercross-pollinated progenies. Notably, the number of individuals containing two different S-haplotypes (S1- and S5-, S5- and S8-, S1- and S4-haplotype was larger than that of other individuals in the two self-pollinated progenies, indicating that some of these hetero-diploid pollen grains may have the capability to inactivate stylar S-RNase inside the pollen tube and grow better into the ovaries.

  14. Cherry Antioxidants: From Farm to Table

    Directory of Open Access Journals (Sweden)

    Alberto Belleggia

    2010-10-01

    Full Text Available The dietary consumption of fruits and vegetables is associated with a lower incidence of degenerative diseases such as cardiovascular disease and certain types of cancers. Most recent interest has focused on the bioactive phenolic compounds found in vegetable products. Sweet and sour cherries contain several antioxidants and polyphenols that possess many biological activities, such as antioxidant, anticancer and anti-inflammation properties. The review describes the effect of environment and other factors (such as production, handling and storage on the nutritional properties of cherries, with particular attention to polyphenol compounds. Moreover the health benefits of cherries and their polyphenols against human diseases such as heart disease, cancers, diabetes are reviewed.

  15. Promising sour cherry hybrids (Prunus cerasus L. developed at Fruit Research Institute Čačak

    Directory of Open Access Journals (Sweden)

    Radičević Sanja

    2010-01-01

    Full Text Available At Fruit Research Institute in Čačak, major objectives of the work on breeding new sour cherry (Prunus cerasus L. cultivars are high cropping, large, high-quality fruits and resistance to causal agents of diseases and pests. As a result of the planned hybridization, more than 10,000 hybrid seedlings have been developed from about 40 cultivars within more than 110 parental combinations, among which are 'Čačanski rubin' ('Shasse Morello' x 'Köröser Weichsel' and 'Šumadinka' ('Köröser Weichsel' x 'Heimanns Konserven Weichsel' which have been named and released. Ten-year study of 11 hybrids, selected from the population of about 3,000 hybrid seedlings, gave four hybrids which have been singled out as elite (III/23, III/31, II/40 i XII/57. These hybrids are currently under procedure of being released as new cultivars. The paper presents two-year results of the study of ripening time, pomological properties, biochemical composition of fruits, and field resistance to causal agents of diseases and pests attacking the above named genotypes which were compared to standard cultivar 'Heimanns Konserven Weichsel'. In the studied hybrids, fruit weight, soluble solids content and sugars content were higher than in standard cultivar. In addition, they exhibit substantial field resistance to causal agents of brown rot (Monilinia laxa /Ader et Ruhl./ Honey ex Whetz., cherry leaf spot (Blumeriella jaapii (Rehm. v. Arx., shot-hole (Clasterosporium carpophilum (Lév. Aderh. and cherry fruit fly (Rhagoletis cerasi L. attack.

  16. Efficiency of enzymatic and other alternative clarification and fining treatments on turbidity and haze in cherry juice

    DEFF Research Database (Denmark)

    Meyer, Anne Boye Strunge; Köser, C.; Adler-Nissen, Jens

    2001-01-01

    function. Individual and interactive effects on turbidity and haze formation in precentrifuged and uncentrifuged cherry juice of treatments with pectinase, acid protease, bromelain, gallic acid, and gelatin-silica sol were investigated in a factorial experimental design with 32 different parameter...... cherry juice with Novozym 89L protease and co- addition of pectinase and gallic acid improved cherry juice clarity and diminished haze levels. None of the alternative treatments produced the unwieldy colloids notorious to gelatin- silica sol treatment. The data suggest that several alternative...

  17. Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis

    Science.gov (United States)

    Sugden, Conor; Velasco-Rodriguez, Óscar; Algarabel-Olona, Miriam; Larrea, Esther; Fernández-Rubio, Celia

    2017-01-01

    Leishmania is the causative agent of leishmaniasis, a neglected tropical disease that affects more than 12 million people around the world. Current treatments are toxic and poorly effective due to the acquisition of resistance within Leishmania populations. Thus, the pursuit for new antileishmanial drugs is a priority. The available methods for drug screening based on colorimetric assays using vital dyes are time-consuming. Currently, the use of fluorescent reporter proteins is replacing the use of viability indicator dyes. We have constructed two plasmids expressing the red fluorescent protein mCherry with multiple cloning sites (MCS), adequate for N- and C-terminal fusion protein constructs. Our results also show that the improved pXG-mCherry plasmid can be employed for drug screening in vitro. The use of the red fluorescent protein, mCherry, is an easier tool for numerous assays, not only to test pharmacological compounds, but also to determine the subcellular localization of proteins. PMID:28286673

  18. Lynne Cherry's World: A Celebration of Nature.

    Science.gov (United States)

    Winarski, Diana L.

    1995-01-01

    Profiles Lynne Cherry, author-illustrator whose books convey her interest in environmentalism and preserving natural resources. Describes her career, process of illustrating several recent books, and founding of the Center for Children's Environmental Literature in 1992. (TM)

  19. Invariant Measures for Cherry Flows

    Science.gov (United States)

    Saghin, Radu; Vargas, Edson

    2013-01-01

    We investigate the invariant probability measures for Cherry flows, i.e. flows on the two-torus which have a saddle, a source, and no other fixed points, closed orbits or homoclinic orbits. In the case when the saddle is dissipative or conservative we show that the only invariant probability measures are the Dirac measures at the two fixed points, and the Dirac measure at the saddle is the physical measure. In the other case we prove that there exists also an invariant probability measure supported on the quasi-minimal set, we discuss some situations when this other invariant measure is the physical measure, and conjecture that this is always the case. The main techniques used are the study of the integrability of the return time with respect to the invariant measure of the return map to a closed transversal to the flow, and the study of the close returns near the saddle.

  20. Invariant measures for Cherry flows

    CERN Document Server

    Saghin, Radu

    2011-01-01

    We investigate the invariant probability measures for Cherry flows, i.e. flows on the two-torus which have a saddle, a source, and no other fixed points, closed orbits or homoclinic orbits. In the case when the saddle is dissipative or conservative we show that the only invariant probability measures are the Dirac measures at the two fixed points, and the Dirac measure at the saddle is the physical measure. In the other case we discuss some situations when there exists another invariant measure supported on the quasi-minimal set, which is the physical measure, and conjecture that this is always the case. The main techniques used are the study of the integrability of the return time with respect to the invariant measure of the return map to a closed transversal to the flow, and the study of the close returns near the saddle.

  1. The effect of rootstocks on the efficiency of a nursery of sweet cherry (Prunus avium L. trees cv. ‘Regina’

    Directory of Open Access Journals (Sweden)

    Piotr Baryła

    2014-01-01

    Full Text Available During the period 2006–2009 in Lublin, a study was conducted to determine the effect of five rootstocks: ‘Colt’, ‘F12/1’, sweet cherry (Prunus avium L., ‘GiSelA 5’, and ‘Piast’, on bud take in the cultivar ‘Regina’, the quality of budded trees and the efficiency of a sweet cherry tree nursery. The highest percentage of bud take in cherry trees cv. ‘Regina’ and the best efficiency of the sweet cherry tree nursery were obtained for the rootstocks ‘Piast’ and ‘Colt’. In two years during the three-year study period, the rootstock was found to significantly affect the efficiency of the sweet cherry tree nursery. When grafted on the rootstocks ‘Colt’ and ‘Piast’, a significantly higher percentage of trees met the requirements of the Polish Standard PN-R-67010 than on the clonal rootstock ‘GiSelA 5’. Under the tested conditions, the quality of maiden sweet cherry trees cv. ‘Regina’ grafted on the dwarfing rootstock ‘GiSelA 5’ was lowest.

  2. Respiratory activity of ‘Chelan’, ‘Bing’ and ‘Selah’ sweet cherries in relation to fruit traits at green, white-pink, red and mahogany ripening stages

    Science.gov (United States)

    Sweet cherry fruit development is subjectively sub-divided into four stages on the basis of exocarp color, which includes green, white-pink, red and mahogany stages. ‘Chelan’, ‘Bing’ and ‘Selah’, representing early-, mid- and late-season cultivars, respectively, and three different abscission-respon...

  3. 7 CFR 923.322 - Washington cherry handling regulation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Washington cherry handling regulation. 923.322 Section... CHERRIES GROWN IN DESIGNATED COUNTIES IN WASHINGTON Order Regulating Handling Grade, Size, Container and Pack Regulation § 923.322 Washington cherry handling regulation. (a) Grade. No handler shall...

  4. 7 CFR 930.8 - Free market tonnage percentage cherries.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Free market tonnage percentage cherries. 930.8 Section... CHERRIES GROWN IN THE STATES OF MICHIGAN, NEW YORK, PENNSYLVANIA, OREGON, UTAH, WASHINGTON, AND WISCONSIN Order Regulating Handling Definitions § 930.8 Free market tonnage percentage cherries. Free...

  5. Evaluation of development of three cultivars of coral bells (Heuchera after application of organic means of improving soil properties and mineral fertilizers on post-mining area of the Dąbrowskie Coal Basin

    Directory of Open Access Journals (Sweden)

    Gabriela Sąkol

    2012-12-01

    Full Text Available The present study investigated the effect of improving soil properties using a liquid organic means: Eko-użyźniacz, based on manure vermicompost, and traditional fertilizer Azofoska on three cultivars of coral bells: ‘Chatterbox’, ‘Marmelade’ and ‘Purple Petticoats’. The plants were field cultivated on post-mining area of the Dąbrowskie Coal Basin from 2009 to 2011. Even though, the doses of nutriens from mean of improve soil properties were low the plants were visually appealing. The effect of Azofoska or Eko-użyźniacz used were not significant (α = 0.05 in diameter of plants, numbers of shoots and inflorescences. All of the cultivars gave more flower shoots in the second vegetation period. Plants of coral bells grow well after a treatment of Eko-użyźniacz, which means that use of this treatment is addequate for post-mining soils.  

  6. Complete nucleotide sequence of little cherry virus 1 (LChV-1) infecting sweet cherry in China

    Science.gov (United States)

    Little cherry virus 1 (LChV-1), associated with little cherry disease (LCD), has a significant impact on fruit quality of infected sweet cherry trees. We report the full genome sequence of an isolate of LChV-1 from China, detected by small RNA deep sequencing and amplified by overlapping RT-PCR. The...

  7. Metabolic profiling of ethephon-treated sweet cherry (Prunus avium L.)

    Science.gov (United States)

    Increasing costs and decreasing labor availability for sweet cherry harvest in Washington State, USA, has reinvigorated commercial and research interest of mechanized harvest. Ethephon (2-chloroethyl phosphonic acid) can be used to improve fruit abscission for mechanical harvest. Our previous work s...

  8. 75 FR 10442 - Sweet Cherries Grown in Designated Counties in Washington; Change in the Handling Regulation

    Science.gov (United States)

    2010-03-08

    ... ``premium'' when handled. This change is expected to reduce market confusion regarding the marketing of such... market confusion and improve producer returns by providing pack differentiation and is expected to... all Washington sweet cherries, but do not ] share the minimum grade requirements with dark...

  9. The effects of the cherry variety on the chemical and sensorial characteristics of cherry brandy

    Directory of Open Access Journals (Sweden)

    NINOSLAV NIKIĆEVIĆ

    2011-09-01

    Full Text Available The chemical and sensorial characteristics of cherry brandy produced from five cherry varieties (Oblacinska, Celery’s 16, Rexle, Heiman’s Ruby and Heiman’s Conserve grown in Serbia were studied. Gas chromatography and gas chromatography–mass spectrometry analysis of these distillates led to the identification of 32 components, including 20 esters, benzaldehyde, 6 terpenes and 5 acids. The ethyl esters of C8–C18 acids were the most abundant in all samples. The benzaldehyde content was quantified by high performance liquid chromatography with UV detection. The average benzaldehyde concentration in the samples ranged between 2.1 and 24.1 mg L-1. The total sensory scores of the cherry brandies ranged between 17.30 to 18.05, with the cherry brandy produced from the Celery’s 16 variety receiving the highest score (18.05.

  10. Tart Cherry Juice as a Treatment for Peripheral Neuropathy

    Science.gov (United States)

    Carson, Cindy Alberts

    2015-01-01

    are on more than 1 of these medications but, even with polypharmacy and optimal dosing, only 40%–60% of patients report partial relief of symptoms, and many have significant adverse effects, such as somnolence, constipation, and drug dependence. Objective Tart cherry juice has been shown to be effective in the treatment of some inflammatory conditions and has a very low incidence of adverse effects. The purpose of this study was to determine whether tart cherry juice is an effective treatment for neuropathic pain from nondiabetic peripheral polyneuropathies. Methods The study group consisted of 12 primary care patient volunteers with painful lower extremity peripheral neuropathy. Patients with diabetic peripheral neuropathy were excluded due to the potentially confounding effect of the increased glycemic load from cherry juice. All study patients had carried a diagnosis of peripheral neuropathy for 5–10 y. All patients described 9–10 out of 10 pain more than 50% of the time. All study patients had been on multiple antiepileptics, analgesics, and antidepressants for more than 2 y without significant improvement of symptoms. All study patients were allowed to remain on their prescribed doses of gabapentin, pregabalin, SNRIs, tricyclics, and SSRIs during the study. The patients agreed not to use NSAIDs, steroids, lidocaine, and narcotic pain relievers during the study. There were no restrictions on diet or activity. Presumed etiologies of the peripheral neuropathies in the study group were: 3 HIV, 2 chemotherapy-induced, 5 spinal disorders, and 2 idiopathic. All patients drank 4 oz of tart cherry juice 2 ×/d for 14 d. The juice used was a combination of Prunus cerasus, Prunus acida, Prunus avium, and Prunus fructicosa, imported from the Caucasus and sold commercially as “100% cherry juice from concentrate.” Results After 14 d of treatment, the patients were questioned regarding the level of their peripheral neuropathy pain. Two patients (HIV and spinal DJD

  11. The Assessment Of The Risk Of Allergenicity Of ‘Sabina’ And ‘Debreceni Bötermö’ Sour Cherry Cvs (Prunus Cerasus L. In A Guinea Pig Model

    Directory of Open Access Journals (Sweden)

    Jasińska-Stroschein Magdalena

    2014-12-01

    Full Text Available The allergic reactions to fruits are lesser known among food sensitivities. The most common fruits belonging to the Rosaceae family that might cause allergic reactions are apples, pears and peaches. However, little is known about the potential allergic reactions caused by another member of the Rosaceae, the cherry. The aim of this study was to assess the risk of any allergic reaction or food hypersensitivity resulting from topical application and chronic oral administration of cherry fruits. The cherry fruits ‘Sabina’ cv. were produced in the orchard in Dąbrowice according to the principles of integrated (IFP and organic (OR productions. Fruits of ‘Debreceni Bötermö’ cv. were produced in Dąbrowice (IFP, and in the orchard in Nowy Dwór (OR. The experiments were performed on 65 outbred young, adult, white albinotic guinea pigs (Dankin Hartley. Three procedures were applied: I. Guinea-Pig Maximization Test (GPMT; II. Chronic administration of fruits and III. Skin prick (Dreborg test. The skin reactions based on GPMT or Dreborg tests revealed no differences between the two cherry cultivars ‘Sabina’ and ‘Debreceni’ obtained from integrated or organic production. Similarly, it was not observed of any effect of cultivars of cherries nor the type of fruits production on the guinea pig skin reaction as a result of chronic feeding with fruits.

  12. Models for the beginning of sour cherry blossom

    Science.gov (United States)

    Matzneller, Philipp; Blümel, Klaus; Chmielewski, Frank-M.

    2014-07-01

    Seven different model approaches to calculate the onset of sour cherry blossom for the main growing regions in Rhineland-Palatinate (Germany) were compared. Three of the approaches were pure forcing models (M1, M2, M2DL) and the remaining four models were combined sequential chilling-forcing (CF) models. Model M1 was the commonly used growing degree day (GDD) model in which the starting date of temperature accumulation ( t 1), the base temperature ( T BF) and the forcing requirement F* were optimized on the basis of observed data. Because of a relatively late optimal starting date ( t 1 = 1 March), the model can be applied only to calculate the onset of cherry blossom for present climate conditions. In order to develop forcing models that could possibly be used to estimate possible shifts in the timing of cherry blossom due to climate change, the starting date t 1 of the models was intentionally set to 1 January (M2, M2DL). Unfortunately, model M2 failed in both the optimization and validation period. The introduction of a daylength term (DL) in model M2DL improved model performance. In order to project possible shifts in the timing of plant phenological events, combined CF-models are preferred over pure GDD-models. For this reason four CF-models were developed with (M3DL, M4DL) and without (M3, M4) consideration of daylength in the GDD-approach. The chilling requirement was calculated using chilling hours (M3, M3DL) and chill portions (M4, M4DL). Both models without daylength estimated implausible model parameters and failed model validation. However, models M3DL and M4DL showed meaningful model parameter estimations and the error between modelled and observed data was markedly reduced. Moreover, the models optimized and validated (internal validation) for one sour cherry growing region in Germany, were applied successfully to calculate the beginning of the blossom period in other regions in Europe and even at one station in North America (external validation).

  13. CULTIVAR RELEASE - BRS Saturno – triticale cultivar

    Directory of Open Access Journals (Sweden)

    Alfredo do Nascimento Junior

    2011-01-01

    Full Text Available The triticale cultivar BRS Saturno was developed by Embrapa (Brazilian Agricultural Research Corporation inpartnership with the Fundação Pró-Sementes de Apoio à Pesquisa. It is the result of a cross between the genotypes PFT 512 and CEP28 – Guará, adapted to the environmental conditions of cultivation in southern Brazil.

  14. Cloning and characterization of genomic DNA sequences of four self-incompatibility alleles in sweet cherry ( Prunus avium L.).

    Science.gov (United States)

    Wünsch, A; Hormaza, J I

    2004-01-01

    Gametophytic self-incompatibility (GSI) in sweet cherry is determined by a locus S with multiple alleles. In the style, the S-locus codifies for an allele-specific ribonuclease ( S-RNase) that is involved in the rejection of pollen that carries the same S allele. In this work we report the cloning and genomic DNA sequence analysis including the 5' flanking regions of four S-RNases of sweet cherry ( Prunus avium L., Rosaceae). DNA from the cultivars Ferrovia, Pico Colorado, Taleguera Brillante and Vittoria was amplified through PCR using primers designed in the conserved sequences of sweet cherry S-RNases. Two alleles were amplified for each cultivar and three of them correspond to three new S-alleles named S23, S24 and S25 present in 'Pico Colorado', 'Vittoria' and 'Taleguera Brillante' respectively. To confirm the identity of the amplified fragments, the genomic DNA of these three putative S-RNases and the allele S12 amplified in the cultivar Ferrovia were cloned and sequenced. The nucleotide and deduced amino-acid sequences obtained contained the structural features of rosaceous S-RNases. The isolation of the 5'-flanking sequences of these four S-RNases revealed a conserved putative TATA box and high similarity among them downstream from that sequence. However, similarity was low compared with the 5'-flanking regions of S-RNases from the Maloideae. S6- and S24-RNase sequences are highly similar, and most amino-acid substitutions among these two RNases occur outside the rosaceous hypervariable region (RHV), but within another highly variable region. The confirmation of the different specificity of these two S-RNases would help elucidate which regions of the S-RNase sequences play a role in S-pollen specific recognition.

  15. SOUR CHERRY (Prunus cerasus L. GENETIC VARIABILITY AND PHOTOSYNTHETIC EFFICIENCY DURING DROUGHT

    Directory of Open Access Journals (Sweden)

    Marija Viljevac

    2012-12-01

    Full Text Available Sour cherry is an important fruit in Croatian orchards. Cultivar Oblačinska is predominant in existing orchards with noted intracultivar phenotypic heterogeneity. In this study, the genetic variability of 22 genotypes of cvs. Oblačinska, Maraska and Cigančica, as well as standard cvs. Kelleris 14, Kelleris 16, Kereška, Rexelle and Heimann conserved were investigated. Two types of molecular markers were used: microsatellite markers (SSR in order to identify intercultivar, and AFLP in order to identify intracultivar variabilities. A set of 12 SSR markers revealed small genetic distance between cvs. Maraska and Oblačinska while cv. Cigančica is affined to cv. Oblačinska. Furthermore, cvs. Oblačinska, Cigančica and Maraska were characterized compared to standard ones. AFLP markers didn`t confirm significant intracultivar variability of cv. Oblačinska although the variability has been approved at the morphological, chemical and pomological level. Significant corelation between SSR and AFLP markers was found. Identification of sour cherry cultivars tolerant to drought will enable the sustainability of fruit production with respect to the climate change in the future. For this purpose, the tolerance of seven sour cherry genotypes (cvs. Kelleris 16, Maraska, Cigančica and Oblačinska represented by 4 genotypes: OS, 18, D6 and BOR to drought conditions was tested in order to isolate genotypes with the desired properties. In the greenhouse experiment, cherry plants were exposed to drought stress. The leaf relative water content, OJIP test parameters which specify efficiency of the photosynthetic system based on measurements of chlorophyll a fluorescence, and concentrations of photo-synthetic pigments during the experiment were measured as markers of drought tolerance. Photosynthetic performance index (PIABS comprises three key events in the reaction centre of photosystem II affecting the photosynthetic activity: the absorption of energy

  16. Tart cherry juice decreases oxidative stress in healthy older men and women.

    Science.gov (United States)

    Traustadóttir, Tinna; Davies, Sean S; Stock, Anthoney A; Su, Yali; Heward, Christopher B; Roberts, L Jackson; Harman, S Mitchell

    2009-10-01

    Compared with young adults, older adults have significantly impaired capacities to resist oxidative damage when faced with acute stress such as ischemia/reperfusion. This impairment likely contributes to increased morbidity and mortality in older adults in response to acute trauma, infections, and the susceptibility to diseases such as atherosclerosis, cancer, diabetes, and Alzheimer's disease. Consumption of foods high in polyphenols, particularly anthocyanins, have been associated with improved health, but the mechanisms contributing to these salutary effects remain to be fully established. This study tested the hypothesis that consumption of tart cherry juice containing high levels of anthocyanins improves the capacity of older adults to resist oxidative damage during acute oxidative stress. In a double-blind, placebo-controlled, crossover design, 12 volunteers [6 men and 6 women; age 69 +/- 4 y (61-75 y)] consumed in random order either tart cherry juice or placebo (240 mL twice daily for 14 d) separated by a 4-wk washout period. The capacity to resist oxidative damage was measured as the changes in plasma F(2)-isoprostane levels in response to forearm ischemia-reperfusion (I/R) before and after each treatment. The tart cherry juice intervention reduced the I/R-induced F(2)-isoprostane response (P tart cherry juice intervention also reduced basal urinary excretion of oxidized nucleic acids (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanosine) (P tart cherry juice improves antioxidant defenses in vivo in older adults as shown by an increased capacity to constrain an oxidative challenge and reduced oxidative damage to nucleic acids.

  17. Effects of a tart cherry juice beverage on the sleep of older adults with insomnia: a pilot study.

    Science.gov (United States)

    Pigeon, Wilfred R; Carr, Michelle; Gorman, Colin; Perlis, Michael L

    2010-06-01

    This study ascertained whether a proprietary tart cherry juice blend (CherryPharm, Inc., Geneva, NY, USA) associated with anecdotal reports of sleep enhancement improves subjective reports of insomnia compared to a placebo beverage. The pilot study used a randomized, double-blind, crossover design where each participant received both treatment and placebo for 2 weeks with an intervening 2-week washout period. Sleep continuity (sleep onset, wake after sleep onset, total sleep time, and sleep efficiency) was assessed by 2-week mean values from daily sleep diaries and disease severity by the Insomnia Severity Index in a cohort of 15 older adults with chronic insomnia who were otherwise healthy. The tart cherry juice beverage was associated with statistically significant pre- to post-treatment improvements on all sleep variables. When compared to placebo, the study beverage produced significant reductions in insomnia severity (minutes awake after sleep onset); no such improvements were observed for sleep latency, total sleep time, or sleep efficiency compared to placebo. Effect sizes were moderate and in some cases negligible. The results of this pilot study suggest that CherryPharm, a tart cherry juice blend, has modest beneficial effects on sleep in older adults with insomnia with effect sizes equal to or exceeding those observed in studies of valerian and in some, but not all, studies of melatonin, the two most studied natural products for insomnia. These effects, however, were considerably less than those for evidence-based treatments of insomnia: hypnotic agents and cognitive-behavioral therapies for insomnia.

  18. Badania biologii pyłku wytwarzanego przez kwiaty wiśni zakwitajace w różnych fazach kwitnienia [Studies of the biology of pollen formed by sour cherry flowers flowering in different stages of the blooming period

    Directory of Open Access Journals (Sweden)

    Stanisław Wociór

    2015-06-01

    Full Text Available During four years (1971-1974, five sour cherry cultivars were examined for the pollen viability, germination and pollen tube growth. Pollen which was taken from the earliest and the last blooming stages had the smaller viability, germination and the shorter pollen tube then the pollen from the flowers at full bloom. Substancial differences of the pollen characters between cultivars and the years of investigations were found.

  19. Dispersal of Rhagoletis cerasi in Commercial Cherry Orchards: Efficacy of Soil Covering Nets for Cherry Fruit Fly Control.

    Science.gov (United States)

    Daniel, Claudia; Baker, Brian

    2013-03-12

    Demand for organic cherries offers producers a premium price to improve their commercial viability. Organic standards require that producers find alternatives to pesticides. Soil treatments to control the European cherry fruit fly Rhagoletis cerasi (L.) (Diptera: Tephrididae) appear to be an attractive option. However, soil treatments can only be effective if the migration of flies is low, because mature flies may migrate from near-by trees for oviposition. To examine the general potential of soil treatments and to understand the dispersal and flight behaviour of R. cerasi within orchards, experiments using netting to cover the soil were conducted in two orchards with different pest pressure during two years. The netting reduced flight activity by 77% and fruit infestation by 91%. The data showed that the flies have a dispersal of less than 5 m within orchards, which is very low. The low thresholds for tolerance for infested fruit in the fresh market creates a strong economic incentive for control, therefore, soil covering is a promising strategy for controlling R. cerasi in commercial orchards.

  20. Tart Cherry Yield and Economic Response to Alternative Planting Densities

    OpenAIRE

    Me-Nsope, Nathalie Mongue

    2009-01-01

    The study investigates the economic response of tart cherry yields to planting density using an unbalanced longitudinal yield data from tart cherry orchards in Northwest Michigan. The relationship between tart cherry yield and tree age is specified as a linear spline function and planting density interacts with tree age. A random effect method, treating block as random, is used to estimate the spline function. Stochastic simulation was used to estimate the mean and variance of the product of ...

  1. Complete nucleotide sequence of a virus associated with rusty mottle disease of sweet cherry (Prunus avium).

    Science.gov (United States)

    Villamor, D V; Druffel, K L; Eastwell, K C

    2013-08-01

    Cherry rusty mottle is a disease of sweet cherries first described in 1940 in western North America. Because of the graft-transmissible nature of the disease, a viral nature of the disease was assumed. Here, the complete genomic nucleotide sequences of virus isolates from two trees expressing cherry rusty mottle disease symptoms are characterized; the virus is designated cherry rusty mottle associated virus (CRMaV). The biological and molecular characteristics of this virus in comparison to those of cherry necrotic rusty mottle virus (CNRMV) and cherry green ring mottle virus (CGRMV) are described. CRMaV was subsequently detected in additional sweet cherry trees expressing symptoms of cherry rusty mottle disease.

  2. Effects of cherry leaf spot on photosynthesis in tart cherry 'Montmorency' foliage.

    Science.gov (United States)

    Gruber, B R; Kruger, E L; McManus, P S

    2012-07-01

    Results described here span a total of three field seasons and quantitatively depict the effects of an economically important fungal pathogen (Blumeriella jaapii) on tart cherry (Prunus cerasus 'Montmorency') leaf physiology. For the first time, leaf photosynthesis, stomatal conductance (g(s)), maximum ribulose-1,5-bisphosphate carboxylation rate (V(cmax)), and maximum electron transport (J(max)) were measured as functions of visible cherry leaf spot disease (CLS) severity. Defined as the proportion of chlorotic and necrotic tissue per leaf, CLS severity was estimated from leaves of mature 'Montmorency' trees in 2007, 2008, and 2009. Briefly, as visible disease severity increased, all of the leaf-level physiological parameters decreased significantly (P tart cherry leaves due to CLS appears to be mediated through disproportionately large perturbations in g(s), V(cmax), and J(max). These findings offer a new perspective on the amount of damage that this serious disease can inflict.

  3. Economic Sustainability of Italian Greenhouse Cherry Tomato

    Directory of Open Access Journals (Sweden)

    Riccardo Testa

    2014-11-01

    Full Text Available Greenhouse tomato cultivation plays an important role in Sicily, being the primary production area in Italy, due to its favorable pedo-climatic conditions that permit extra-seasonal productions. In Sicily, more than half of greenhouse tomato production is derived from the Province of Ragusa on the southeastern coast, where especially cherry tomato typologies are cultivated. Over the last decade, the Ragusa Province has registered a decrease both in terms of greenhouse tomato area and harvested production due to several structural problems that would require restructuring of the tomato supply chain. Thus, since recognition of real costs and profitability of tomato growing is a vital issue, both from the perspective of the farm, as well as from that of the entrepreneur, the aim of this paper was to analyze the economic sustainability of Sicilian greenhouse cherry tomato cultivated in the Ragusa Province. In particular, an economic analysis on 30 representative farms was conducted in order to estimate production costs and profits of greenhouse cherry tomato. According to our results, the lack of commercial organization, which characterizes the small farms we surveyed, determines low contractual power for farmers and, consequently, low profitability.

  4. Identification of chilling and heat requirements of cherry trees—a statistical approach

    Science.gov (United States)

    Luedeling, Eike; Kunz, Achim; Blanke, Michael M.

    2013-09-01

    Most trees from temperate climates require the accumulation of winter chill and subsequent heat during their dormant phase to resume growth and initiate flowering in the following spring. Global warming could reduce chill and hence hamper the cultivation of high-chill species such as cherries. Yet determining chilling and heat requirements requires large-scale controlled-forcing experiments, and estimates are thus often unavailable. Where long-term phenology datasets exist, partial least squares (PLS) regression can be used as an alternative, to determine climatic requirements statistically. Bloom dates of cherry cv. `Schneiders späte Knorpelkirsche' trees in Klein-Altendorf, Germany, from 24 growing seasons were correlated with 11-day running means of daily mean temperature. Based on the output of the PLS regression, five candidate chilling periods ranging in length from 17 to 102 days, and one forcing phase of 66 days were delineated. Among three common chill models used to quantify chill, the Dynamic Model showed the lowest variation in chill, indicating that it may be more accurate than the Utah and Chilling Hours Models. Based on the longest candidate chilling phase with the earliest starting date, cv. `Schneiders späte Knorpelkirsche' cherries at Bonn exhibited a chilling requirement of 68.6 ± 5.7 chill portions (or 1,375 ± 178 chilling hours or 1,410 ± 238 Utah chill units) and a heat requirement of 3,473 ± 1,236 growing degree hours. Closer investigation of the distinct chilling phases detected by PLS regression could contribute to our understanding of dormancy processes and thus help fruit and nut growers identify suitable tree cultivars for a future in which static climatic conditions can no longer be assumed. All procedures used in this study were bundled in an R package (`chillR') and are provided as Supplementary materials. The procedure was also applied to leaf emergence dates of walnut (cv. `Payne') at Davis, California.

  5. Effective pollination period in "Oblačinska" sour cherry clones

    Directory of Open Access Journals (Sweden)

    Fotirić-Akšić Milica

    2014-01-01

    Full Text Available To obtain high yields there should be high flower density and fruit set in sour cherry (Prunus cerasus L. production. Furthermore, in order to ensure successful fertilization, there should be satisfactory stigma receptivity, rapid pollen tube growth along the style, as well as adequate ovule longevity. This manuscript presents the study of the effective pollination period (EPP of four ‘Oblačinska’ sour cherry clones (II/2, III/9, XI/3 and XIII/1 that differs in pollen germination, fruit set and yields. In order to estimate EPP, pollination was conducted in six different stages of flower development: balloon stage, 2 d before anthesis (-2, at anthesis (0, and 2, 4, 6 and 8 d after anthеsis (DAA. The initial (IFS and final fruit set (FFS were recorded under the field conditions. Alongside with this, the rate of pollen tubes growth in the style was observed with fluorescent microscopy. The experimental design was completely randomized, a two-factorial analysis of variance was carried out and individual testing was performed using LSD test (p ≤ 0.05; p ≤ 0.01. The experiment was set in triplicates. Regarding FFS, clones II/2 and III/9 showed the best results (p ≤ 0.01 in 4 and 6 DAA. The number of pollen tubes in the style of the pistil decreased with subsequent terms of pollination, while its number in the ovule increased up to sixth day after pollination, followed by a decline. Clones II/2 and III/9 showed EPP which lasted from 6 to 8 d, while EPP found in clone XI/3, lasted only 2 d. It is concluded that only clone having long EPP should be used as parents for creating new sour cherry cultivars. [Projekat Ministarstva nauke Republike Srbije, br. TR 31063 and FP7 Project AREA 316004

  6. Development and evaluation of a genome-wide 6K SNP array for diploid sweet cherry and tetraploid sour cherry.

    Directory of Open Access Journals (Sweden)

    Cameron Peace

    Full Text Available High-throughput genome scans are important tools for genetic studies and breeding applications. Here, a 6K SNP array for use with the Illumina Infinium® system was developed for diploid sweet cherry (Prunus avium and allotetraploid sour cherry (P. cerasus. This effort was led by RosBREED, a community initiative to enable marker-assisted breeding for rosaceous crops. Next-generation sequencing in diverse breeding germplasm provided 25 billion basepairs (Gb of cherry DNA sequence from which were identified genome-wide SNPs for sweet cherry and for the two sour cherry subgenomes derived from sweet cherry (avium subgenome and P. fruticosa (fruticosa subgenome. Anchoring to the peach genome sequence, recently released by the International Peach Genome Initiative, predicted relative physical locations of the 1.9 million putative SNPs detected, preliminarily filtered to 368,943 SNPs. Further filtering was guided by results of a 144-SNP subset examined with the Illumina GoldenGate® assay on 160 accessions. A 6K Infinium® II array was designed with SNPs evenly spaced genetically across the sweet and sour cherry genomes. SNPs were developed for each sour cherry subgenome by using minor allele frequency in the sour cherry detection panel to enrich for subgenome-specific SNPs followed by targeting to either subgenome according to alleles observed in sweet cherry. The array was evaluated using panels of sweet (n = 269 and sour (n = 330 cherry breeding germplasm. Approximately one third of array SNPs were informative for each crop. A total of 1825 polymorphic SNPs were verified in sweet cherry, 13% of these originally developed for sour cherry. Allele dosage was resolved for 2058 polymorphic SNPs in sour cherry, one third of these being originally developed for sweet cherry. This publicly available genomics resource represents a significant advance in cherry genome-scanning capability that will accelerate marker-locus-trait association discovery

  7. Characterization and mapping of non-S gametophytic self-compatibility in sweet cherry (Prunus avium L.).

    Science.gov (United States)

    Cachi, A M; Wünsch, A

    2011-03-01

    Self-incompatibility in Prunus (Rosaceae) species, such as sweet cherry, is controlled by a multiallelic locus (S), in which two tightly linked genes, S-RNase and SFB (S haplotype-specific F-box), determine the specificity of the pollen and the style. Fertilization in these species occurs only if the S-specificities expressed in the pollen and the pistils are different. However, modifier genes have been proposed to be necessary for a full manifestation of the self-incompatibility response. 'Cristobalina' is a spontaneous self-compatible sweet cherry cultivar that originated in Eastern Spain. Previous studies with this genotype suggested that pollen modifier gene(s), not linked to the S-locus, may be the cause of self-incompatibility breakdown. In this work, an F(1) population from 'Cristobalina' that segregates for this trait was used to identify molecular markers linked to self-compatibility by bulked segregant analysis. One simple sequence repeat (SSR) locus (EMPaS02) was found to be linked to self-compatibility in this population at 3.2 cM. Two additional populations derived from 'Cristobalina' were used to confirm the linkage of this marker to self-compatibility. Since EMPaS02 has been mapped to the sweet cherry linkage group 3, other markers located on the same linkage group were analysed in these populations to confirm the location of the self-compatibility locus.

  8. Construction of Commercial Sweet Cherry Linkage Maps and QTL Analysis for Trunk Diameter.

    Science.gov (United States)

    Wang, Jing; Zhang, Kaichun; Zhang, Xiaoming; Yan, Guohua; Zhou, Yu; Feng, Laibao; Ni, Yang; Duan, Xuwei

    2015-01-01

    A cross between the sweet cherry (Prunus avium) cultivars 'Wanhongzhu' and 'Lapins' was performed to create a mapping population suitable for the construction of a linkage map. The specific-locus amplified fragment (SLAF) sequencing technique used as a single nucleotide polymorphism (SNP) discovery platform and generated 701 informative genotypic assays; these, along with 16 microsatellites (SSRs) and the incompatibility (S) gene, were used to build a map which comprised 8 linkage groups (LGs) and covered a genetic distance of 849.0 cM. The mean inter-marker distance was 1.18 cM and there were few gaps > 5 cM in length. Marker collinearity was maintained with the established peach genomic sequence. The map was used to show that trunk diameter (TD) is under the control of 4 loci, mapping to 3 different LGs. Different locus influenced TD at a varying stage of the tree's development. The high density 'W×L' genetic linkage map has the potential to enable high-resolution identification of QTLs of agronomically relevant traits, and accelerate sweet cherry breeding.

  9. Construction of Commercial Sweet Cherry Linkage Maps and QTL Analysis for Trunk Diameter.

    Directory of Open Access Journals (Sweden)

    Jing Wang

    Full Text Available A cross between the sweet cherry (Prunus avium cultivars 'Wanhongzhu' and 'Lapins' was performed to create a mapping population suitable for the construction of a linkage map. The specific-locus amplified fragment (SLAF sequencing technique used as a single nucleotide polymorphism (SNP discovery platform and generated 701 informative genotypic assays; these, along with 16 microsatellites (SSRs and the incompatibility (S gene, were used to build a map which comprised 8 linkage groups (LGs and covered a genetic distance of 849.0 cM. The mean inter-marker distance was 1.18 cM and there were few gaps > 5 cM in length. Marker collinearity was maintained with the established peach genomic sequence. The map was used to show that trunk diameter (TD is under the control of 4 loci, mapping to 3 different LGs. Different locus influenced TD at a varying stage of the tree's development. The high density 'W×L' genetic linkage map has the potential to enable high-resolution identification of QTLs of agronomically relevant traits, and accelerate sweet cherry breeding.

  10. Physicochemical Composition, Phenolic Content and Antioxidant Activity of Sour Cherry cv. Marasca During Ripening

    Directory of Open Access Journals (Sweden)

    Sandra Pedisić

    2007-12-01

    Full Text Available Sour cherry cv. Marasca is Dalmatian cultivar from XVI century. Cultivation is limited on the north and central part of Dalmatia and on the part of the islands, where it achieves the best quality of fruit, high content of dry matter and sugar respectively, agreeable aroma and intense color. Sour cherry cv. Marasca is source of biologically active ingredients, organic and inorganic compounds, dietary fibers, aromatic compounds and high content of phenolic compounds, particularly anthocyanins and hormone melatonin. Many epidemiological studies showed that phenolic compounds have antioxidant and anti-inflammatory properties, and they have beneficial effect on human health. Marasca is rich with mentioned compounds. Physicochemical composition (total and soluble dry matter, pH value, total acidity during ripening of cv. Marasca ecotype Recta (Cerasus marasca recta grown in Zadar and Split area were determined. Total and soluble dry matter increased and pH value and total acidity showed little change during ripening. The content of phenolic compounds increased during ripening as well as content of anthocyanins, while antioxidant activity decreased with ripening. That indicates there was no correlation between antioxidant activity and content of total phenolic compounds.

  11. The relationship between the anthocyanin and vitamin C contents of red-fleshed sweet cherries and the ability of fruit digests to reduce hydrogen peroxide-induced oxidative stress in Caco-2 cells.

    Science.gov (United States)

    Leong, Sze Ying; Burritt, David J; Hocquel, Alice; Penberthy, Ari; Oey, Indrawati

    2017-07-15

    The present work investigated the bioprotective capacities of red-fleshed sweet cherry cultivars (Prunus avium; Lapins, Stella, Sweetheart and Staccato), with distinct differences in anthocyanins and vitamin C contents, on human intestinal Caco-2 cells exposed to hydrogen peroxide (H2O2)-induced oxidative stress. Three assays of cell health, the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl-tetrazolium-bromide cell viability assay, the lactate dehydrogenase membrane leakage assay and nitric oxide production, were used to determine if these cherry digests differed in their ability to protect Caco-2 cells from H2O2. Cells treated with digests from cherries identified as containing high anthocyanins provided the greatest protection against H2O2. A strong linear correlation (-0.82stress than vitamin C.

  12. The Study of Adaptation of the New Peach Cultivars in the Agricultural Region of Vlora

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    BARDHOSH FERRAJ

    2015-10-01

    Full Text Available Drupaceous fruits are placed in the first place in the species structure of Albania’s farms. To anticipate problems in the development of arboriculture and market production, scientific institutions have started a research to find possible solutions for the development of this sector. This is the reason why through collaboration between, CTT Vlore & IAM Bari was made possible to bring from Italy during the years 2000-2001, 96 cultivars of peaches, plums, cherries, apricots, nectarines, almonds. These cultivars dominate the production markets of the Mediterranean countries. In this project were included 21 peach cultivars 10 years old, rootstocks, planting material of basic category, clear of viruses and other pathogens. As the main rootstocks were selected GF 677 and MRS 2/5. To study the behavior, adaptability and productivity of the peach cultivars, in the warm coastal areas, specialists from the Agriculture University of Tirana and Center for Technology Transfer in Vlora carried out a number of tests and evaluations during a three year period. At the end of the study it resulted that the cultivars with a very early maturation better adapted in the coastal areas were: ‘Cardinal’, ‘May Crest’, and ‘Spring Crest’. The cultivars with early maturation and consolidated productivity were: “Red Haven”, “Bolero”, “Dixired”, “Early Crest”, “Iris Rosso”. Cultivars with an average maturation period were: “Red Haven Bianca”, “Red Top”, “Regina Bianca”, “Sun Crest”. The most suitable cultivars with a delayed maturation period are considered: “Big Moon”, “Domiziana”, “Haile”, “Laure”, “Maria Delizia”, “Rome Star”, “Pontina” and “Red Cal”. The only cultivar with a very delayed maturation is “Padana”.

  13. A Near Infrared Spectroscopy (NIRS) and Chemometric Approach to Improve Apple Fruit Quality Management: A Case Study on the Cultivars "Cripps Pink" and "Braeburn".

    Science.gov (United States)

    Eisenstecken, Daniela; Panarese, Alessia; Robatscher, Peter; Huck, Christian W; Zanella, Angelo; Oberhuber, Michael

    2015-07-24

    The potential of near infrared spectroscopy (NIRS) in the wavelength range of 1000-2500 nm for predicting quality parameters such as total soluble solids (TSS), acidity (TA), firmness, and individual sugars (glucose, fructose, sucrose, and xylose) for two cultivars of apples ("Braeburn" and "Cripps Pink") was studied during the pre- and post-storage periods. Simultaneously, a qualitative investigation on the capability of NIRS to discriminate varieties, harvest dates, storage periods and fruit inhomogeneity was carried out. In order to generate a sample set with high variability within the most relevant apple quality traits, three different harvest time points in combination with five different storage periods were chosen, and the evolution of important quality parameters was followed both with NIRS and wet chemical methods. By applying a principal component analysis (PCA) a differentiation between the two cultivars, freshly harvested vs. long-term stored apples and, notably, between the sun-exposed vs. shaded side of apples could be found. For the determination of quality parameters effective prediction models for titratable acid (TA) and individual sugars such as fructose, glucose and sucrose by using partial least square (PLS) regression have been developed. Our results complement earlier reports, highlighting the versatility of NIRS as a fast, non-invasive method for quantitative and qualitative studies on apples.

  14. A Near Infrared Spectroscopy (NIRS and Chemometric Approach to Improve Apple Fruit Quality Management: A Case Study on the Cultivars “Cripps Pink” and “Braeburn”

    Directory of Open Access Journals (Sweden)

    Daniela Eisenstecken

    2015-07-01

    Full Text Available The potential of near infrared spectroscopy (NIRS in the wavelength range of 1000–2500 nm for predicting quality parameters such as total soluble solids (TSS, acidity (TA, firmness, and individual sugars (glucose, fructose, sucrose, and xylose for two cultivars of apples (“Braeburn” and “Cripps Pink” was studied during the pre- and post-storage periods. Simultaneously, a qualitative investigation on the capability of NIRS to discriminate varieties, harvest dates, storage periods and fruit inhomogeneity was carried out. In order to generate a sample set with high variability within the most relevant apple quality traits, three different harvest time points in combination with five different storage periods were chosen, and the evolution of important quality parameters was followed both with NIRS and wet chemical methods. By applying a principal component analysis (PCA a differentiation between the two cultivars, freshly harvested vs. long-term stored apples and, notably, between the sun-exposed vs. shaded side of apples could be found. For the determination of quality parameters effective prediction models for titratable acid (TA and individual sugars such as fructose, glucose and sucrose by using partial least square (PLS regression have been developed. Our results complement earlier reports, highlighting the versatility of NIRS as a fast, non-invasive method for quantitative and qualitative studies on apples.

  15. Detecting pits in tart cherries by hyperspectral transmission imaging

    Science.gov (United States)

    Qin, Jianwei; Lu, Renfu

    2004-11-01

    The presence of pits in processed cherry products causes safety concerns for consumers and imposes potential liability for the food industry. The objective of this research was to investigate a hyperspectral transmission imaging technique for detecting the pit in tart cherries. A hyperspectral imaging system was used to acquire transmission images from individual cherry fruit for four orientations before and after pits were removed over the spectral region between 450 nm and 1,000 nm. Cherries of three size groups (small, intermediate, and large), each with two color classes (light red and dark red) were used for determining the effect of fruit orientation, size, and color on the pit detection accuracy. Additional cherries were studied for the effect of defect (i.e., bruises) on the pit detection. Computer algorithms were developed using the neural network (NN) method to classify the cherries with and without the pit. Two types of data inputs, i.e., single spectra and selected regions of interest (ROIs), were compared. The spectral region between 690 nm and 850 nm was most appropriate for cherry pit detection. The NN with inputs of ROIs achieved higher pit detection rates ranging from 90.6% to 100%, with the average correct rate of 98.4%. Fruit orientation and color had a small effect (less than 1%) on pit detection. Fruit size and defect affected pit detection and their effect could be minimized by training the NN with properly selected cherry samples.

  16. 21 CFR 152.126 - Frozen cherry pie.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen cherry pie. 152.126 Section 152.126 Food... pie. (a) Identity. (1) Frozen cherry pie (excluding baked and then frozen) is the food prepared by... ingredients as prescribed by paragraph (a)(2) of this section. The finished food is frozen. (2) The...

  17. First Report of Cherry virus A in Sweet Cherry Trees in China

    Science.gov (United States)

    Plants in the genus Prunus of the family Rosaceae are important ornamental and fruit trees in China (1). In June 2007, sweet cherry (Prunus avium) trees with mottling and mosaic symptoms were observed in a private garden near Kunming, Yunnan Province. Twenty-four samples were then collected from swe...

  18. The quality of sweet cherries stored under hypobaric conditions

    Directory of Open Access Journals (Sweden)

    H. Borecka

    2013-12-01

    Full Text Available Sweet cherries cvs 'Emperor Francis' and 'Grosse Schwarze Knorpelkirsche' were stored under hypobaric conditions, 5 kPa and 25 kPa at 2°C. These sweet cherries could be stored for 30 days without a high losses. Sweet cherries of both cvs tasted the best after storage under LPS, worse under CA, and the worst in normal air. Titrate acidity decreased during the storage period, the percent of soluble solids decreased for 15 days after which it increased. Mostly Botrytis cinerea and Monilinia spp. destroyed the sweet cherries during storage. Penicillium spp. affected fruits by less than 1% under kPa, and those subsequently stored for 3 days at 20°C. Hypobaric storage 5 kPa at 2°C seems to be patricularly good for storage of sweet cherries cv. 'Emperor Francis'.

  19. Tart Cherry Extracts Reduce Inflammatory and Oxidative Stress Signaling in Microglial Cells

    OpenAIRE

    Shukitt-Hale, Barbara; Megan E. Kelly; Donna F. Bielinski; Fisher, Derek R.

    2016-01-01

    Tart cherries contain an array of polyphenols that can decrease inflammation and oxidative stress (OS), which contribute to cognitive declines seen in aging populations. Previous studies have shown that polyphenols from dark-colored fruits can reduce stress-mediated signaling in BV-2 mouse microglial cells, leading to decreases in nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression. Thus, the present study sought to determine if tart cherries—which improved cogn...

  20. Oviposition in Sweet Cherry by Reproductively Mature Western Cherry Fruit Fly (Tephritidae:Diptera) Fed Spinosad and Neonicotinoid Insecticide Baits

    Science.gov (United States)

    Western cherry fruit fly, Rhagoletis indifferens Curran, is a major pest of cherry, Prunus avium (L.) L., in the Pacific Northwest of the U.S. Spinosad bait is applied weekly to kill flies before they develop eggs, but its effects on oviposition by flies that are reproductively mature are unknown. ...

  1. Development and evaluation of a genome-wide 6K SNP array for diploid sweet cherry and tetraploid sour cherry

    Science.gov (United States)

    High-throughput genome scans are important tools for genetic studies and breeding applications. Here, a 6K SNP array for use with the Illumina Infinium® system was developed for diploid sweet cherry (Prunus avium) and allotetraploid sour cherry (P. cerasus). This effort was led by RosBREED, a commun...

  2. Cracking susceptibility of sweet cherries (Prunus avium L.) under different conditions.

    Science.gov (United States)

    Mucha-Pelzer, T; Müller, S; Rohr, F; Mewis, I

    2006-01-01

    Rain and hail during ripening and harvesting season can cause yield losses up to 90 % in sweet cherry cultivations in Germany. Particularly, high yield losses after precipitation are due to the cracking and the following rotting process through bacteria and fungi's. In order to protect the crop and improve cherry quality they can be treated with chemicals dried or healthier covered with rain shelters. To date the cracking phenomenon of cherries is still not clearly understood. Therefore, in the present study the cracking resistance of three cherry varieties under different conditions was observed. We used 'Regina', 'Karina', and 'Summit' grafted on the rootstock GiSelA 5. The test-section in the field was covered with a plastic-foil (pc) as rain shelter and a bird net, whereby the control (c) was covered with a bird protection-net only. The cherry varieties have been harvested, weight and sorted into undamaged, rotten and cracked fruits. In order to compare the varieties under equal conditions the cracking sensitivity was also tested under laboratory conditions with the method from CHRISTENSEN (1996). The average yield per tree was significantly higher in the pc treatment in 'Summit' and 'Regina' than in the control. Furthermore, the average weight of rotten fruits per tree in these varieties was in c, without rain shelter, about two to three times higher as in the pc treatment. There was no significant difference in yield and rotten fruits among treatments in 'Karina'. But the percentage of cracked fruits between the cultivation methods was significant different. Hereby improvement of cherry quality was observed in the pc treatment with reduced numbers of useless fruits. Under field conditions 'Karina' was at least susceptible to cracking resistance followed by 'Regina' and 'Summit'. In the laboratory Cracking Test the result was different. Here, the variety 'Summit' showed the best cracking resistance compared to the other varieties. According to the method of

  3. Pessegueiro: cultivar BRS kampai

    Directory of Open Access Journals (Sweden)

    Maria do Carmo Bassols Raseira

    2010-12-01

    Full Text Available RESUMO O programa de Melhoramento Genético de Pessegueiros da Embrapa Clima Temperado tem, entre seus objetivos, a obtenção de cultivares produtoras de frutas para consumo in natura, com características que satisfaçam às exigências dos consumidores. Alguns dos grandes centros consumidores, como é o caso de São Paulo e Curitiba, preferem pêssegos de polpa branca e sabor doce. A cultivar BRS Kampai, obtida de um cruzamento entre 'Chimarrita' e 'Flordaprince', alia a baixa necessidade em frio, o que é uma vantagem em regiões subtropicais, à boa aparência, com sabor superior a qualquer um dos parentais. A colheita dos frutos desta cultivar inicia-se geralmente, em meados de novembro, em Pelotas-RS (em Atibaia, São Paulo, inicia-se na segunda quinzena de outubro, poucos dias antes das cultivares Rubimel (polpa amarela e Premier (polpa branca, sendo ótima substituta para esta última.

  4. 7 CFR 930.54 - Prohibition on the use or disposition of inventory reserve cherries.

    Science.gov (United States)

    2010-01-01

    ...), DEPARTMENT OF AGRICULTURE TART CHERRIES GROWN IN THE STATES OF MICHIGAN, NEW YORK, PENNSYLVANIA, OREGON, UTAH... reserve cherries. 930.54 Section 930.54 Agriculture Regulations of the Department of Agriculture... disposition of inventory reserve cherries. Cherries that are placed in inventory reserve pursuant to...

  5. The Cherry Trees in Our School

    Institute of Scientific and Technical Information of China (English)

    吕迪; 吕铁雄

    2004-01-01

    “The cherry Trees in Our School”是吕迪同学描述自己校园的一篇文章,通过对樱花的描述,使得整篇文章内容生动、语言流畅,充分体现了他对事物细腻的观察力,对生活的热爱,对提供他学习环境的肯定。在此刊登全文,以飨读者。

  6. Characterization of the Newly Developed Soybean Cultivar DT2008 in Relation to the Model Variety W82 Reveals a New Genetic Resource for Comparative and Functional Genomics for Improved Drought Tolerance

    Directory of Open Access Journals (Sweden)

    Chien Van Ha

    2013-01-01

    Full Text Available Soybean (Glycine max productivity is adversely affected by drought stress worldwide, including Vietnam. In the last few years, we have made a great effort in the development of drought-tolerant soybean cultivars by breeding and/or radiation-induced mutagenesis. One of the newly developed cultivars, the DT2008, showed enhanced drought tolerance and stable yield in the field conditions. The purpose of this study was to compare the drought-tolerant phenotype of DT2008 and Williams 82 (W82 by assessing their water loss and growth rate under dehydration and/or drought stress conditions as a means to provide genetic resources for further comparative and functional genomics. We found that DT2008 had reduced water loss under both dehydration and drought stresses in comparison with W82. The examination of root and shoot growths of DT2008 and W82 under both normal and drought conditions indicated that DT2008 maintains a better shoot and root growth rates than W82 under both two growth conditions. These results together suggest that DT2008 has better drought tolerance degree than W82. Our results open the way for further comparison of DT2008 and W82 at molecular levels by advanced omic approaches to identify mutation(s involved in the enhancement of drought tolerance of DT2008, contributing to our understanding of drought tolerance mechanisms in soybean. Mutation(s identified are potential candidates for genetic engineering of elite soybean varieties to improve drought tolerance and biomass.

  7. Adding food baits to expand the list of insecticide classes used to manage Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) in cherry

    Science.gov (United States)

    Drosophila suzukii has become a major pest of fruit crops, including cherry in the western United States. We evaluated whether the addition of sugary baits could improve the efficacy of two classes of insecticides not considered to be sufficiently effective for this pest, diamides and spinosyns, in ...

  8. Self-compatibility in 'Cristobalina' sweet cherry is not associated with duplications or modified transcription levels of S-locus genes.

    Science.gov (United States)

    Wünsch, A; Tao, R; Hormaza, J I

    2010-07-01

    Sweet cherry shows S-RNase-based gametophytic self-incompatibility, which prevents self- and cross-fertilization between genetically related individuals. The specificity of the self-incompatible reaction is determined by two genes located in the S-locus. These encode a pistil-expressed ribonuclease (S-RNase) that inhibits self pollen tube growth, and a pollen-expressed F-box protein (SFB) that may be involved in the cytotoxicity of self-S-RNases. Initial genetic and pollination studies in a self-compatible sweet cherry cultivar, 'Cristobalina' (S (3) S (6)), showed that self-compatibility was caused by the loss of pollen function of both haplotypes (S (3) and S (6)). In this study, we further characterize self-compatibility in this genotype by molecular analysis of the S-locus. DNA blot analyses using S-RNase and SFB probes show no duplications of 'Cristobalina' S-locus genes or differences in the restriction patterns when compared with self-incompatible cultivars with the same S-genotype. Furthermore, reverse transcriptase-PCR of S-locus genes and quantitative reverse transcription-PCR of SFBs revealed no differences at the transcription level when compared with a self-incompatible genotype. The results of this study show that no differences at the S-locus can be correlated with self-compatibility, indicating the possible involvement of non-S-locus modifiers in self-incompatibility breakdown in this cultivar.

  9. Phenolic metabolism in grafted versus nongrafted cherry tomatoes under the influence of water stress.

    Science.gov (United States)

    Sánchez-Rodríguez, Eva; Ruiz, Juan Manuel; Ferreres, Federico; Moreno, Diego A

    2011-08-24

    Use of grafts using rootstocks capable of palliating the effects of water stress can be a possible solution to reduce yield losses. For response to stress, plants can induce the metabolism of phenylpropanoids. The aim of the present work is to determine the response of reciprocal grafts made between one tolerant cherry tomato cultivar, Zarina, and a more sensitive cultivar, Josefina. The analysis of the phenylpropanoids pathway was carried out both enzymatically and metabolically. DAHP synthase, shikimate dehydrogenase, phenylalanine ammonium-lyase, cinnamate 4-hydroxylase, and 4-coumarate CoA ligase activities were determined, and characteristic metabolites from the pathway were measured by means of HPLC-MS. Growth in the grafts JosxZar and ZarxJos was not appreciably affected by stress. JosxZar had increased concentrations of phenolic compounds after water stress. This could be correlated with the greater activity of synthesis enzymes as well as a decrease in phenol-degrading enzymes. Phenolic metabolism is more influenced by the aerial part, and therefore it is concluded that the capacity of inducing tolerance in rootstocks depends on the genotype of the shoot.

  10. Micropropagation of peach rootstocks and cultivars.

    Science.gov (United States)

    Balla, Ildikó; Mansvelt, Lucienne

    2013-01-01

    Peach (Prunus persica (L.) Batsch) is one of the most popular stone fruits, commercially produced largely in Mediterranean and, to a lesser extent, in continental climatic conditions. Several breeding programs with different aims release annually large numbers of new cultivars. Micropropagation offers a suitable method to provide the growers of sufficient quantities of rootstocks, as well as of pathogen-free planting material of old and new cultivars.An effective four-step micropropagation procedure for cultivar and rootstock production is described here, based on the use of modified MS and WPM media. The health status of the initial shoot tips is very important, also because the growth and proliferation rate of shoot cultures from virus-infected clones are generally very poor. Proliferation and elongation phases depend on the major macro-elements, as well as the content and ratio of plant growth regulators. It is important to grow the cultures at 22°C, as hyperhydricity may develop at higher temperatures. Although sucrose is the most common carbon source used during proliferation and rooting, for some peach cultivars and rootstocks the replacement of sucrose (10 g/L) with glucose (20 g/L) in the rooting medium improves the rooting and survival rates of plants in the acclimatization phase. The rooting rate of the rootstock "Cadaman" is improved with the chelated form of iron FeEDDHA at 150 mg/L. Rooted plants are acclimatized in greenhouse under high humidity conditions.

  11. Differences in body size and egg loads of Rhagoletis indifferens (Diptera: Tephritidae) from introduced and native cherries

    Science.gov (United States)

    The western cherry fruit fly, Rhagoletis indifferens Curran, infests introduced, domesticated sweet [Prunus avium (L.) L.] and tart cherries (Prunus cerasus L.) as well as native bitter cherry, Prunus emarginata (Douglas) Eaton. Bitter cherries are smaller than sweet and tart cherries and this coul...

  12. Production and quality of tomato fruits under organic management Produção e qualidade dos frutos de cultivares de tomateiro, sob manejo orgânico

    Directory of Open Access Journals (Sweden)

    Débora S Toledo

    2011-06-01

    Full Text Available Information about the production and quality of tomato cultivars under organic management and the conditions of climate and soil from the north region of Minas Gerais State, Brasil, are scarce. Thus, this research was carried out to evaluate the production and quality of tomato cultivars under organic management, in this region. The treatments consisted of four open pollination tomato cultivars (Chadwick Cherry, Pitanga vermelha, Santa Clara and Santa Cruz Kada and five hybrid cultivars (Marguerita, Nicolas, Ellen, Magestade and Dominador. The experimental design was of randomized blocks with three replications. The experiment was carried out using organic fertilization in the initial preparation of the area, compost and rock phosphate and sprays with Bordeaux mixture, neem oil and fertilizer. The marketable yield varied from 12.3 t ha-1 to 23.9 t ha-1. The hybrid Marguerita presented higher marketable production (23.9 t ha-1. The cultivars Chadwick Cherry and Pitanga Vermelha reached similar production as most cultivars and larger proportions of marketable and giant fruits, and therefore, better market quality. All the cultivars presented low potassium and manganese levels in foliar tissue suggesting an evaluation after various organic manuring cycles and better nutrition conditions are necessary to permit the comparison of the performance of cultivars in this system.Informações sobre a produção e qualidade de frutos de cultivares de tomateiro, sob manejo orgânico, nas condições de clima e solo do Norte de Minas Gerais, Brasil, são escassas. Por isso, o objetivo deste trabalho foi avaliar a produção e qualidade de frutos de cultivares de tomateiro, sob manejo orgânico, em Montes Claros-MG. Os tratamentos consistiram de quatro cultivares de tomate de polinização livre (Chadwick Cherry, Pitanga Vermelha, Santa Clara e Santa Cruz Kada e cinco cultivares híbridos (Marguerita, Nícolas, Ellen, Majestade e Dominador. Foi utilizado o

  13. Root density of cherry trees grafted on prunus mahaleb in a semi-arid region

    Science.gov (United States)

    Paltineanu, Cristian; Septar, Leinar; Gavat, Corina; Chitu, Emil; Oprita, Alexandru; Moale, Cristina; Lamureanu, Gheorghe; Vrinceanu, Andrei

    2016-07-01

    Root density was investigated using the trench method in a cherry (Prunus avium grafted on Prunus mahaleb) orchard with clean cultivation in inter-rows and in-row. Trenches of 1 m width and 1.2 m depth were dug up between neighbouring trees. The objectives of the paper were to clarify the spatial distribution of root density of cherry trees under the soil and climate conditions of the region to expand knowledge of optimum planting distance and orchard management for a broad area of chernozems. Some soil physical properties were significantly worsened in inter-rows versus in-row, mainly due to soil compaction, and there were higher root density values in in-row versus inter-rows. Root density decreased more intensely with soil depth than with distance from trees. The pattern of root density suggests that the cherry tree density and fruit yield could be increased. However, other factors concerning orchard management and fruit yield should also be considered. The results obtained have a potential impact to improve irrigation and fertilizer application by various methods, considering the soil depth and distance from trees to wet soil, in accordance with root development.

  14. Cherry: development of a non traditional crop in Argentina

    OpenAIRE

    Scarpati, Olga; Maio, Silvina; Puga, Yamile

    2011-01-01

    The incorporation of non-traditional crops for Argentine Republic became notable in the late XX century and early XXI by technological innovations that allow very significant yields, the opportunity represented by the exportation of agricultural products, as is the case of cherry, favoured by the exchange rate, and against seasonality of production for consumer markets in the Northern Hemisphere. The main cherry-growing areas are three: the Uco Valley in Mendoza province, the Los Antiguos Val...

  15. Seasonal distributions of the western cherry fruit fly (Diptera: Tephritidae) among host and nonhost fruit trees.

    Science.gov (United States)

    Yee, Wee L

    2014-01-01

    Seasonal distributions of the western cherry fruit fly, Rhagoletis indifferens Curran (Diptera: Tephritidae), in sweet cherry (Prunus avium (L.) L.) (major host), black hawthorn (occasional developmental host) (Crataegus douglasii Lindley), and other trees were determined in a ponderosa pine ecosystem in Washington state, USA. The hypothesis that most fly dispersal from cherry trees occurs after fruit senesce or drop was tested, with emphasis on movement to black hawthorn trees. Sweet cherry fruit developed earlier than black hawthorn, bitter cherry (common host), choke cherry, and apple fruit. Flies were usually captured first in sweet cherry trees but were caught in bitter cherry and other trees throughout the season. Peak fly capture periods in sweet cherry began around the same time or slightly earlier than in other trees. However, peak fly capture periods in black hawthorn and other nonsweet cherry trees continued after peak periods in sweet cherry ended, or relative fly numbers within sweet cherry declined more quickly than those within other trees. Larvae were reared from sweet and bitter cherry but not black hawthorn fruit. Results provide partial support for the hypothesis in that although R. indifferens commonly disperses from sweet cherry trees with fruit, it could disperse more, or more flies are retained in nonsweet cherry trees after than before sweet cherries drop. This could allow opportunities for the flies to use other fruit for larval development. Although R. indifferens infestation in black hawthorn was not detected, early season fly dispersal to this and other trees and fly presence in bitter cherry could make fly management in sweet cherry difficult.

  16. Effect of Different Photoperiods on Cold Hardiness in Cherry

    Directory of Open Access Journals (Sweden)

    A.Z. Makaraci

    2009-01-01

    Full Text Available In this research we have investigated the effects of different photoperiods on cold hardiness of cherry trees (Prunus avium cv. Ulster. One year old, cherry trees were exposed 8 hours of day length and 16 hours of dark period for short day conditions and 16 hours of light and 8 hours of dark period for long day conditions. We also compared the acclimatization pattern of cherry trees with hybrid aspen (Populus tremula ×Populus tremuloides trees. LT50 values indicated that short day and long day conditions did not alter the cold hardiness of cherry trees. Under the same experimental conditions hybrid aspen trees were affected from different photoperiods. Aspen trees exposed to short day conditions were hardier than the trees exposed to long day conditions. We also investigated the acclimatization the patterns of basal and apical parts of the branches both in cherry and aspen trees. There were no significant differences in cold hardiness of the basal and apical parts of the branches in aspen and cherry trees.

  17. Are commercial sweet cherry rootstocks adapted to climate change? Short-term waterlogging and CO2 effects on sweet cherry cv. 'Burlat'.

    Science.gov (United States)

    Pérez-Jiménez, Margarita; Hernández-Munuera, María; Piñero, M Carmen; López-Ortega, Gregorio; Del Amor, Francisco M

    2017-01-20

    High CO2 is able to ameliorate some negative effects due to climate change and intensify others. This study involves the sweet cherry (Prunus avium) cultivar 'Burlat' grafted on the 'Mariana 2624', 'Adara' and 'LC 52' rootstocks. In a climate chamber at two CO2 concentrations: ambient (400 µmol mol(-1) ) and elevated (800 µmol mol(-1) ), the plants were submitted to waterlogging for seven days, followed by seven days of recovery after drainage. Waterlogging drastically decreased the rate of photosynthesis, significantly endangering plant survival, particularly for the 'LC 52' and 'Adara' rootstocks. 'Mariana 2624' was also clearly affected by waterlogging, that increased lipid peroxidation and the Cl(-) and SO4(2-) concentrations in all the studied plants. Nevertheless, CO2 was able to overcome this reduction in photosynthesis, augmenting growth, increasing soluble sugars and starch, raising turgor and regulating the concentrations of Cl(-) and SO4(2-) , while lowering the NO3(-) concentration in leaves of all the studied rootstocks. In concordance with these results, the proline levels indicated a more intense stress at control CO2 than at high CO2 for waterlogged plants. 'Mariana 2624' was more resistant to waterlogging than 'Adara' and both were more resistant than 'LC 52' in control CO2 conditions; this clearly enhanced the chance of survival under hypoxia.

  18. 1H NMR metabolic fingerprinting to probe temporal postharvest changes on qualitative attributes and phytochemical profile of sweet cherry fruit

    Directory of Open Access Journals (Sweden)

    Vlasios eGoulas

    2015-11-01

    Full Text Available Sweet cherry fruits (Prunus avium cvs. ‘Canada Giant’, ‘Ferrovia’ were harvested at commercial maturity stage and analyzed at harvest and after maintenance at room temperature (storage at ~ 20°C, shelf life for 1, 2, 4, 6 and 8 days, respectively. Fruit were initially analyzed for respiration rate, qualitative attributes and textural properties: ‘Canada Giant’ fruit were characterized by higher weight losses and stem browning index, being more intense over the late stages of shelf life period; meanwhile ‘Ferrovia’ possessed appreciably better performance even after extended shelf life period. A gradual decrease of respiration rate was monitored in both cultivars, culminated after 8 days at 20°C. The sweet cherry fruit nutraceutical profile was monitored using an array of instrumental techniques (spectrophotometric assays, HPLC, 1H-NMR. Fruit antioxidant capacity was enhanced with the progress of shelf life period, concomitant with the increased levels of total anthocyanin and of phenolic compounds. ‘Ferrovia’ fruit presented higher contents of neochlorogenic acid and p-coumarolquinic acid throughout the shelf life period. We further developed an 1H-NMR method that allows the study of primary and secondary metabolites in a single running, without previous separation and isolation procedures. Diagnostic peaks were located in the aliphatic region for sugars and organic acids, in the aromatic region for phenolic compounds and at 8.2 to 8.6 ppm for anthocyanins. This NMR-based methodology provides a unifying tool for quantitative and qualitative characterization of metabolite changes of sweet cherry fruits; it is also expected to be further exploited for monitoring temporal changes in other fleshy fruits.

  19. Expression and Anthocyanin Biosynthesis-Modulating Potential of Sweet Cherry (Prunus avium L.) MYB10 and bHLH Genes.

    Science.gov (United States)

    Starkevič, Pavel; Paukštytė, Jurgita; Kazanavičiūtė, Vaiva; Denkovskienė, Erna; Stanys, Vidmantas; Bendokas, Vidmantas; Šikšnianas, Tadeušas; Ražanskienė, Aušra; Ražanskas, Raimundas

    2015-01-01

    Anthocyanins are essential contributors to fruit coloration, an important quality feature and a breed determining trait of a sweet cherry fruit. It is well established that the biosynthesis of anthocyanins is regulated by an interplay of specific transcription factors belonging to MYB and bHLH families accompanied by a WD40 protein. In this study, we isolated and analyzed PaWD40, PabHLH3, PabHLH33, and several closely related MYB10 gene variants from different cultivars of sweet cherry, analyzed their expression in fruits with different anthocyanin levels at several developmental stages, and determined their capabilities to modulate anthocyanin synthesis in leaves of two Nicotiana species. Our results indicate that transcription level of variant PaMYB10.1-1 correlates with fruit coloration, but anthocyanin synthesis in Nicotiana was induced by another variant, PaMYB10.1-3, which is moderately expressed in fruits. The analysis of two fruit-expressed bHLH genes revealed that PabHLH3 enhances MYB-induced anthocyanin synthesis, whereas PabHLH33 has strong inhibitory properties.

  20. Anthocyanin composition, antioxidant efficiency, and α-amylase inhibitor activity of different Hungarian sour cherry varieties (Prunus cerasus L.).

    Science.gov (United States)

    Homoki, Judit R; Nemes, Andrea; Fazekas, Erika; Gyémánt, Gyöngyi; Balogh, Péter; Gál, Ferenc; Al-Asri, Jamil; Mortier, Jérémie; Wolber, Gerhard; Babinszky, László; Remenyik, Judit

    2016-03-01

    Five Hungarian sour cherry cultivars were studied to determine their anthocyanin contents and their possible inhibitory properties. The water and methanol soluble antioxidant capacities were separately assessed by photoluminescence showing values ranged from 3.4μgmg(-1) to 15.4μgmg(-1), respectively. The "VN1" variety (selected from "Csengődi csokros") showed the highest antioxidant capacity. The anthocyanin content, measured by pH differential method or isolated by solid phase extraction, was the highest also in "VN1". Correlation was found between the anthocyanin content and the high antioxidant capacity. The main anthocyanin components were cyanidin-3-O-rutinoside and cyanidin-3-O-glucoside. The presence of malvidin-3,5-O-diglycoside was verified by MALDI-TOF MS. Sour cherry extracts and selected anthocyanins inhibited the human salivary alpha-amylase catalyzed hydrolysis competitively. The lowest IC50 value, 55μgmL(-1) or 80μM, was measured for malvidin-3,5-O-diglycoside, for which possible binding modes within the alpha-amylase active site could be investigated in silico using molecular docking and molecular dynamics.

  1. Expression and Anthocyanin Biosynthesis-Modulating Potential of Sweet Cherry (Prunus avium L. MYB10 and bHLH Genes.

    Directory of Open Access Journals (Sweden)

    Pavel Starkevič

    Full Text Available Anthocyanins are essential contributors to fruit coloration, an important quality feature and a breed determining trait of a sweet cherry fruit. It is well established that the biosynthesis of anthocyanins is regulated by an interplay of specific transcription factors belonging to MYB and bHLH families accompanied by a WD40 protein. In this study, we isolated and analyzed PaWD40, PabHLH3, PabHLH33, and several closely related MYB10 gene variants from different cultivars of sweet cherry, analyzed their expression in fruits with different anthocyanin levels at several developmental stages, and determined their capabilities to modulate anthocyanin synthesis in leaves of two Nicotiana species. Our results indicate that transcription level of variant PaMYB10.1-1 correlates with fruit coloration, but anthocyanin synthesis in Nicotiana was induced by another variant, PaMYB10.1-3, which is moderately expressed in fruits. The analysis of two fruit-expressed bHLH genes revealed that PabHLH3 enhances MYB-induced anthocyanin synthesis, whereas PabHLH33 has strong inhibitory properties.

  2. BRS 137: cultivar de soja para cultivo no Sul do Brasil BRS 137: soybean cultivar for southern Brazil

    Directory of Open Access Journals (Sweden)

    Emídio Rizzo Bonato

    2001-05-01

    Full Text Available A liberação de cultivares de elevado potencial produtivo e com resistência a doenças é fundamental para continuar agregando rendimento na produção brasileira de soja. A cultivar BRS 137 é resultado do programa de melhoramento de soja desenvolvido pela Embrapa. A cultivar apresentou rendimento médio de grãos 2% superior ao da cultivar IAS 5, em 24 ambientes, no Rio Grande do Sul. É resistente à pústula-bacteriana (Xanthomonas axonopodis pv. glycines, ao cancro-da-haste (Diaporthe phaseolorum f. sp. meridionalis, à podridão-parda-da-haste (Phialophora gregata, à mancha-olho-de-rã (Cercospora sojina e ao oídio (Microsphaera diffusa. É indicada para cultivo no Rio Grande do Sul, em semeaduras realizadas a partir de meados de outubro até fim de novembro, com população máxima de 300.000 plantas/ha.The goals of the soybean breeding program for the release of a new cultivar include high yield potential and resistance to diseases in order to improve Brazilian production. The cultivar BRS 137 is a result of the soybean breeding program of Embrapa (Brazil. Prior to its release, the cultivar had shown a mean grain yield potential 2% higher than the cultivar IAS 5 in 24 environments in the State of Rio Grande do Sul, Brazil. Moreover, it is resistant to bacterial pustule (Xanthomonas axonopodis pv. glycines, soybean stem canker (Diaporthe phaseolorum f. sp. meridionalis, brown stem rot (Phialophora gregata, frogeye leaf spot (Cercospora sojina, and powdery mildew (Microsphaera diffusa. The cultivar is being indicated to be cropped in the Rio Grande do Sul state for sowing from mid October to the end of November with a maximum population of 300,000 plants/ha.

  3. Exploitation of sweet cherry (Prunus avium L.) puree added of stem infusion through fermentation by selected autochthonous lactic acid bacteria.

    Science.gov (United States)

    Di Cagno, Raffaella; Surico, Rosalinda Fortunata; Minervini, Giovanna; Rizzello, Carlo Giuseppe; Lovino, Raffaella; Servili, Maurizio; Taticchi, Agnese; Urbani, Sefania; Gobbetti, Marco

    2011-08-01

    Strains of Lactobacillus plantarum, Pediococcus acidilactici, Pediococcus pentosaceus and Leuconostoc mesenteroides subsp. mesenteroides were identified from 8 cultivars of sweet cherry by partial 16S rRNA gene sequence and subjected to typing by Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) analysis. Representative isolates from each species and each cultivar were screened based on the kinetics of growth on cherry puree added of (10%, v/v) stem infusion (CP-SI). A protocol for processing and storage of CP-SI, which included fermentation by selected autochthonous P. pentosaceus SWE5 and L. plantarum FP3 (started CP-SI) or spontaneous fermentation (unstarted CP-SI), was set up. Starters grew and remained viable at elevated cell numbers (ca. 9.0 log cfu g(-1)) during 60 days of storage at 4 °C. The number of presumptive lactic acid bacteria of the unstarted CP-SI did not exceed the value of ca. 3.0 log cfu g(-1). Consumption of carbohydrates (e.g., glucose and fructose) by starter lactic acid bacteria was limited as well as it was the lactic acid fermentation. Consumption of organic acids (e.g., malic acid) and free amino acids was evident, especially, throughout storage. Compared to CP-SI before processing, the concentrations of total phenolic compounds and anthocyanins did not vary in the started CP-SI. The concentration of anthocyanins slightly decreased in the unstarted CP-SI. The antioxidant activity, expressed as the scavenging activity toward DPPH radical, was found at highest level in the started CP-SI which approached that found in CP-SI before processing. During storage, viscosity and, especially, color indexes of started CP-SI were higher than those found in the unstarted CP-SI. Fermentation by autochthonous lactic acid bacteria seemed to also positively interfere with the sensory attributes of CP-SI.

  4. Application of Hazard Analysis and Critical Control Points in Cherry Juice Processing Enterprises

    OpenAIRE

    Peilong Xu; Na Na

    2015-01-01

    Qingdao is one of the homelands for Cherry in China and in recent years, deep processing industry of cherry is developing rapidly. In this study, Hazard Analysis and Critical Control Points (HACCP) quality control system is introduced into production process of cherry juice, which has effectively controlled food safety risks in food production processes. The practices have proved that application of HACCP system reduced probability of pollution in cherry juice production process effectively. ...

  5. Differences in body size and egg loads of Rhagoletis indifferens (Diptera: Tephritidae) from introduced and native cherries.

    Science.gov (United States)

    Yee, Wee L; Goughnour, Robert B; Feder, Jeffrey L

    2011-12-01

    The western cherry fruit fly, Rhagoletis indifferens Curran, infests introduced, domesticated sweet [Prunus avium (L.) L.], and tart cherries (Prunus cerasus L.) as well as native bitter cherry, Prunus emarginata (Douglas) Eaton. Bitter cherries are smaller than sweet and tart cherries and this could affect various life history traits of flies. The objectives of the current study were to determine 1) if body size and egg loads of flies infesting sweet, tart, and bitter cherries differ from one another; and 2) if any observed body size differences are genetically based or caused by the host fruit environment. Pupae and adults of both sexes reared from larval-infested sweet and tart cherries collected in Washington and Montana were larger than those reared from bitter cherries. In addition, flies of both sexes caught on traps in sweet and tart cherry trees were larger than those caught in bitter cherry trees and females trapped from sweet and tart cherry trees had 54.0-98.8% more eggs. The progeny of flies from naturally-infested sweet and bitter cherries reared for one generation in the laboratory on sweet cherry did not differ in size. The same also was true for progeny of sweet and bitter cherry flies reared in the field on bitter cherry. The results suggest that the larger body sizes of flies from sweet and tart cherries than bitter cherries in the field are caused by host fruit and not genetic factors.

  6. The effect of foliar fertilization on growth and yield of sour cherry (Prunus cerasus L. cv. Łutówka

    Directory of Open Access Journals (Sweden)

    Stanisław Wociór

    2012-12-01

    Full Text Available The present study, carried out in the period 2007-2009 in the Sandomierz Upland, did not show a significant effect of foliar fertilization on trunk thickness and canopy volume in the cultivar Łutówka. However, under the influence of urea applied three times in combination with Florovit at a concentration of 0.5%, a clear increasing trend was observed in the above mentioned parameters used to assess the strength of growth. In years of abundant fruiting, this study showed a significant increase, or on the verge of significance, in yield after threefold application of urea and Florovit in combination. A small insignificant effect of fertilization on fruit weight shows that the growth in yield after the application of fertilization was produced as a consequential effect of a significant increase in the length of one-year shoots and thereby in flowering intensity of the 'Łutówka' cherry trees. In the case of the sour cherry cultivar Łutówka bearing fruit on one-year shoots, foliar feeding after flowering using multi-nutrient fertilizers and urea can be recommended as a technological treatment, in particular in years of abundant fruiting.

  7. 33 CFR 208.82 - Hetch Hetchy, Cherry Valley, and Don Pedro Dams and Reservoirs.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Hetch Hetchy, Cherry Valley, and..., Cherry Valley, and Don Pedro Dams and Reservoirs. The Turlock Irrigation District and Modesto Irrigation..., shall operate Hetch Hetchy Dam and Reservoir and Cherry Valley Dam and Reservoir in the interest...

  8. 7 CFR 930.29 - Eligibility for membership on Cherry Industry Administrative Board.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Eligibility for membership on Cherry Industry... AGRICULTURE TART CHERRIES GROWN IN THE STATES OF MICHIGAN, NEW YORK, PENNSYLVANIA, OREGON, UTAH, WASHINGTON... Cherry Industry Administrative Board. (a) Each grower member and each grower alternate member of...

  9. 7 CFR 52.784 - Score sheet for canned red tart pitted cherries.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Score sheet for canned red tart pitted cherries. 52.784 Section 52.784 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING... Pitted Cherries 1 Score Sheet § 52.784 Score sheet for canned red tart pitted cherries. Size and kind...

  10. 76 FR 65357 - Tart Cherries Grown in Michigan, New York, Pennsylvania, Oregon, Utah, Washington, and Wisconsin...

    Science.gov (United States)

    2011-10-21

    ... Service 7 CFR Part 930 Tart Cherries Grown in Michigan, New York, Pennsylvania, Oregon, Utah, Washington... prescribed under the marketing order for tart cherries (order). The order regulates the handling of tart... amended (7 CFR part 930), regulating the handling of tart cherries grown in Michigan, New...

  11. 75 FR 77564 - Tart Cherries Grown in the States of Michigan, et al.;

    Science.gov (United States)

    2010-12-13

    ... Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, et al.; Final Free and Restricted Percentages for the 2010-2011 Crop Year for Tart Cherries AGENCY: Agricultural... handling of tart cherries grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah,...

  12. 76 FR 69673 - Tart Cherries Grown in Michigan, New York, Pennsylvania, Oregon, Utah, Washington, and Wisconsin...

    Science.gov (United States)

    2011-11-09

    ...; ] DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in Michigan, New... tart cherries grown in Michigan, New York, Pennsylvania, Oregon, Utah, Washington, and Wisconsin. These... handling of tart cherries grown in Michigan, New York, Pennsylvania, Oregon, Utah, Washington,...

  13. 76 FR 42072 - Tart Cherries Grown in Michigan, New York, Pennsylvania, Oregon, Utah, Washington, and Wisconsin...

    Science.gov (United States)

    2011-07-18

    ... Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in Michigan, New York, Pennsylvania, Oregon... changes to the grower diversion regulations prescribed under the marketing order for tart cherries (order). The order regulates the handling of tart cherries grown in the States of Michigan, New...

  14. 77 FR 13015 - Tart Cherries Grown in Michigan, New York, Pennsylvania, Oregon, Utah, Washington, and Wisconsin...

    Science.gov (United States)

    2012-03-05

    ... Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in Michigan, New York, Pennsylvania, Oregon... the handling of tart cherries grown in Michigan, New York, Pennsylvania, Oregon, Utah, Washington, and... 40 handlers of tart cherries subject to regulation under the order and approximately 600 producers...

  15. 7 CFR 52.802 - Grades of frozen red tart pitted cherries.

    Science.gov (United States)

    2010-01-01

    ... OTHER PROCESSED FOOD PRODUCTS 1 United States Standards for Grades of Frozen Red Tart Pitted Cherries Product Description and Grades § 52.802 Grades of frozen red tart pitted cherries. (a) “U.S. Grade A” (or... 7 Agriculture 2 2010-01-01 2010-01-01 false Grades of frozen red tart pitted cherries....

  16. 7 CFR 52.812 - Score sheet for frozen red tart pitted cherries.

    Science.gov (United States)

    2010-01-01

    ..., AND CERTAIN OTHER PROCESSED FOOD PRODUCTS 1 United States Standards for Grades of Frozen Red Tart Pitted Cherries Score Sheet § 52.812 Score sheet for frozen red tart pitted cherries. Size and kind of... 7 Agriculture 2 2010-01-01 2010-01-01 false Score sheet for frozen red tart pitted cherries....

  17. Industrial processing effects on phenolic compounds in sour cherry (Prunus cerasus L.) fruit

    NARCIS (Netherlands)

    Toydemir, G.; Capanoglu, E.; Gomez-Roldan, M.V.; Vos, de R.C.H.; Boyacioglu, D.; Hall, R.D.; Beekwilder, M.J.

    2013-01-01

    The processed juice (or nectar) of the sour cherry, Prunus cerasus L., is widely consumed in the Balkan region and Turkey. Sour cherry is known to be rich in polyphenolic compounds, such as anthocyanins and procyanidins. In this work, the effects of processing of sour cherry fruit to nectar on polyp

  18. A new self-compatibility haplotype in the sweet cherry 'Kronio', S5', attributable to a pollen-part mutation in the SFB gene.

    Science.gov (United States)

    Marchese, Annalisa; Bosković, Radovan I; Caruso, Tiziano; Raimondo, Antonio; Cutuli, Marcello; Tobutt, Kenneth R

    2007-01-01

    'Kronio' is a Sicilian cultivar of sweet cherry (Prunus avium), nominally with the incompatibility genotype S(5)S(6), that is reported to be naturally self-compatible. In this work the cause of its self-compatibility was investigated. Test selfing confirmed self-compatibility and provided embryos for analysis; PCR with consensus primers designed to amplify S-RNase and SFB alleles showed that the embryos were of two types, S(5)S(5) and S(5)S(6), indicating that S(6) pollen failed, but S(5) succeeded, perhaps because of a mutation in the pollen or stylar component. Stylar RNase analysis indicated active S-RNases for both S(5) and S(6). The S-RNase alleles were cloned and sequenced; and sequences encode functional proteins. Cloning and sequencing of SFB alleles showed that S(6) was normal but S(5) had a premature stop codon upstream of the variable region HVa resulting in a truncated protein. Therefore, the self-compatibility can be attributed to a pollen-part mutation of S(5), designated S(5)', the first reported case of breakdown of self-incompatibility in diploid sweet cherry caused by a natural mutation at the S-locus. The second intron of the S-RNase associated with S(5)' contained a microsatellite smaller than that associated with S(5); primers designed to amplify across this microsatellite effectively distinguished S(5) from S(5)'. Analysis of some other Sicilian cherries with these primers indicated that S(5)' is also present in the Sicilian cultivar 'Maiolina a Rappu', and this proved to be self-compatible.

  19. IDENTIFICATION OF KEY MOLECULAR COMPONENTS OF THE RESISTANCE OF CHERRY TOMATO AGAINSTPhytophthora infestans Identificación de los principales componentes moleculares de la resistencia de tomate cherry contra Phytophthora infestans

    Directory of Open Access Journals (Sweden)

    LILIANA LÓPEZ KLEINE

    2012-12-01

    Full Text Available Cherry tomato Solanum lycopersicum var cerasiforme cv Matt's wild cherry is a very resistant cultivar to most Phytophthora infestans isolates. Two isolates were identified, US940480 and US970001 that cause an incompatible and a compatible interaction respectively. US970001 is one of the few isolates producing a compatible interaction with this cultivar. To identify genes with a differential gene expression between compatible and incompatible interactions, gene expression patterns were analyzed with tomato cDNA microarrays including 12,899 independent tomato cDNA clones at different time points after inoculation. A diverse set of statistical tools were used to identify key components of the plant response to the pathogen. Forty-three genes were up-regulated during the incompatible reaction at time point 36 hours, 15 globally at all time points and twelve were found both in globally and at 36 hours. Northern blots analysis was performed to confirm differential expression showed by microarray analysis and to study the differential expression of more plant resistance genes (PR genes between compatible and incompatible interactions for this interaction.El tomate cherry Solanum lycopersicum var cerasiforme cv Matt's es bastante resistente a la gran parte de aislamientos de Phytophthora infestans. Se han identificado dos aislamientos, US940480 y US970001 que causan interacción incompatible y compatible respectivamente. US970001 es uno de los pocos aislamientos causantes de interacción compatible con este cultivo. Con el fin de identificar genes con expresión diferencial en interacciones compatible e incompatible, analizamos DNA copia de 12899 clones independientes en tres tiempos posteriores a la inoculación del patógeno. Se aplicaron diversas herramientas estadísticas para identificar componentes moleculares claves de la respuesta de la planta al patógeno. Cuarenta y tres genes fueron detectados como activados durante la interacci

  20. Pessegueiro: cultivar BRS kampai Peach cultivar BRS kampai

    Directory of Open Access Journals (Sweden)

    Maria do Carmo Bassols Raseira

    2010-12-01

    Full Text Available RESUMO O programa de Melhoramento Genético de Pessegueiros da Embrapa Clima Temperado tem, entre seus objetivos, a obtenção de cultivares produtoras de frutas para consumo in natura, com características que satisfaçam às exigências dos consumidores. Alguns dos grandes centros consumidores, como é o caso de São Paulo e Curitiba, preferem pêssegos de polpa branca e sabor doce. A cultivar BRS Kampai, obtida de um cruzamento entre 'Chimarrita' e 'Flordaprince', alia a baixa necessidade em frio, o que é uma vantagem em regiões subtropicais, à boa aparência, com sabor superior a qualquer um dos parentais. A colheita dos frutos desta cultivar inicia-se geralmente, em meados de novembro, em Pelotas-RS (em Atibaia, São Paulo, inicia-se na segunda quinzena de outubro, poucos dias antes das cultivares Rubimel (polpa amarela e Premier (polpa branca, sendo ótima substituta para esta última.The peach breeding program of Embrapa Clima Temperado has, among the objectives the development of fresh market cultivars that fulfill the consumer's preference. Some of the largest consumer centers, such as São Paulo and Curitiba, prefer white flesh peaches with sweet flavor. Cultivar BRS Kampai originated from a cross between 'Chimarrita' and 'Flordaprince', adds the low chilling requirement, advantageous for subtropical areas, to the good appearance and flavor superior to any of the parents. The harvest period of this cultivar begins mid November, in Pelotas, RS, a few days before Rubimel (yellow flesh and Premier (white flesh cultivars (and in the second half of October, in Atibaia, SP being a good substitute for the later.

  1. Deterioration of sweet cherry fruit during storage period

    Directory of Open Access Journals (Sweden)

    Halina Borecka

    2013-12-01

    Full Text Available Sweet cherry fruit cv. 'Emperor Francis' and 'Grosse Schwarze Knorpelkirsche' could be stored in cold storage conditions with normal air without big loss. Fruits stored under CA conditions were less affected by fungi during storage period, but later when were kept for 3 days at 20°C were more deteriorated than those stored at the same temperature in normal air. Botrytis cinerea and Monilinia spp. were the main fungi causing rot of sweet cherry fruit. Low temperature decreased the development of Monilinia spp. The most important factor for good fruit storage was the size of baskets. Sweet cherries stored in small baskets containing 0.5 kg of fruits were of good quality after one month of storage; in 2-kg boxes they were more damaged.

  2. Propriedades do pólen e do estigma de Ocimum basilicum L. (cultivar Maria Bonita para aumentar a eficiência de cruzamentos em programas de melhoramento Ocimum basilicum L. (cultivar Maria Bonita pollen and stigma properties to improve the efficiency of crossings in plant breeding programs

    Directory of Open Access Journals (Sweden)

    A.C Brito

    2010-06-01

    Full Text Available Ocimum basilicum, cv. Maria Bonita (Lamiaceae, conhecido como manjericão, é espécie que apresenta propriedades aromáticas, condimentares e medicinais, por ser rico emóleos essenciais. É muito usado nas indústrias farmacêuticas e de alimentos em geral. O objetivo deste trabalho foi estudar as propriedades do pólen e estigma do manjericão (cultivar Maria Bonita identificando procedimentos simples que possam contribuir para programas de melhoramento. Para análise da disponibilidade, viabilidade do pólen e receptividade de estigma, botões florais foram coletados de hora em hora ao longo do dia, e lâminas eram montadas e coradas, para observação em microscópio óptico. Foi verificado que o manjericão apresenta antese diurna, assincrônica e com a maioria das flores se abrindo entre 10:00 e 11:00 horas. Quanto ao estudo do pólen foi verificado que a viabilidade manteve-se elevada ao longo do dia e a conservação por até 90 dias demonstrou bons níveis de viabilidade. O estigma apresentou receptividade desde a pré-antese até a pós-antese. Estas informações são relevantes para os melhoristas que desejam fazer seleção de genótipos ou hibridações em programas de melhoramento, contribuindo para aumentar o potencial da espécie que já se destaca como produtora de óleos essenciais.Known as basil, Ocimum basilicum cv. Maria Bonita (Lamiaceae is a species that presents aromatic, condimental and medicinal properties, since it is rich in essential oils. This species is largely used in pharmaceutical and food industries. The aim of this work was to study basil (cultivar Maria Bonita pollen and stigma properties, identifying simple procedures that can contribute to plant breeding programs. To analyze pollen availability and viability, besides stigma receptivity, flower buds were collected at every hour throughout the day, and slides were mounted, stained and observed under an optical microscope. Basil presented diurnal

  3. 76 FR 13528 - Tart Cherries Grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah, Washington...

    Science.gov (United States)

    2011-03-14

    ... Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, New York... Marketing Agreement and Order No. 930 (order), which regulate the handling of tart cherries grown in... agreements, Reporting and recordkeeping requirements, Tart cherries. PART 930--TART CHERRIES GROWN IN...

  4. 77 FR 40250 - Tart Cherries Grown in the States of Michigan, et al.; Increasing the Primary Reserve Capacity...

    Science.gov (United States)

    2012-07-09

    ... Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, et al.; Increasing... order for tart cherries (order). The order regulates the handling of tart cherries grown in the States... locally by the Cherry Industry Administrative Board (Board). This action increases the volume of...

  5. Bioactive components of Prunus avium L. black gold (red cherry) and Prunus avium L. stark gold (white cherry) juices, wines and vinegars.

    Science.gov (United States)

    Budak, Nilgün H

    2017-01-01

    Cherries are one of the most popular fruits, characterized by attractive colour, firmness, appearance and delicious tastes. Cherries are consumed fresh as well as in jams, wine, dried, candy and other processed products. Cherries vary in antioxidant properties and phenolic substances. The aim of the study was to determine the effects of ethanol and acetic acid fermentation on total antioxidant activities and phenolic substances of cherry juice. Total investigation of solids, pH, soluble solids, phenolic substances, ORAC and TEAC of Prunus avium L. cherry juices, macerated cherries wine, and vinegars were analyzed. All samples had 300.1-854.79 mg GAE/L of total phenolic contents, and 6.62-17.97 µmol/mL of ORAC values, and 1.5-5.5 mmol/mL of TEAC. Chlorogenic acid was present in the highest amount P. avium L. black gold vinegar.

  6. High-Throughput Screening of Sensory and Nutritional Characteristics for Cultivar Selection in Commercial Hydroponic Greenhouse Crop Production

    Directory of Open Access Journals (Sweden)

    Atef M. K. Nassar

    2015-01-01

    Full Text Available Hydroponic greenhouse-grown and store-bought cultivars of tomato (cherry and beefsteak, cucumbers, bibb lettuce, and arugula were investigated to see if they could be distinguished based on sensory qualities and phytonutrient composition. Only the more dominant sensory criteria were sufficiently robust to distinguish between cultivars and could form the core of a consolidated number of criteria in a more discriminating sensory evaluation test. Strong determinants for cultivar selection within each crop included the following: mineral analysis (particularly Cu, Fe, K, Mg, and P; total carotenoids (particularly β-carotene, lycopene, and lutein; total carbohydrate (except in arugula; organic acids; total phenolics and total anthocyanins (except in cucumber. Hydroponically grown and store-bought produce were of similar quality although individual cultivars varied in quality. Storage at 4°C for up to 6 days did not affect phytonutrient status. From this, we conclude that “freshness,” while important, has a longer duration than the 6 days used in our study. Overall, the effect of cultivar was more important than the effect of growing method or short-term storage at 4°C under ideal storage conditions.

  7. Application of Hazard Analysis and Critical Control Points in Cherry Juice Processing Enterprises

    Directory of Open Access Journals (Sweden)

    Peilong Xu

    2015-09-01

    Full Text Available Qingdao is one of the homelands for Cherry in China and in recent years, deep processing industry of cherry is developing rapidly. In this study, Hazard Analysis and Critical Control Points (HACCP quality control system is introduced into production process of cherry juice, which has effectively controlled food safety risks in food production processes. The practices have proved that application of HACCP system reduced probability of pollution in cherry juice production process effectively. The application of risk control system in cherry juice production provides benefits for standardization of the production process and helps in food safety supervision in production processes.

  8. Reproductive maturity of cherry fruit fly (Diptera: Tephritidae) in managed and natural habitats.

    Science.gov (United States)

    Teixeira, Luís A F; Gut, Larry J; Isaacs, Rufus; Alston, Diane G

    2009-08-01

    We studied the timing of reproductive maturity of cherry fruit fly, Rhagoletis cingulata (Loew), a key pest of sweet and tart cherries in the eastern United States. To determine when cherry fruit fly females become reproductively mature in managed and natural habitats, we deployed traps in sweet and tart cherry orchards and nearby stands of the ancestral host tree, black cherry. Flies were removed from the traps and females were dissected to determine the presence of fully developed eggs. We found that capture of reproductively mature female flies occurred earlier in orchards that are not sprayed with insecticides than in sprayed orchards or in black cherry tree sites. In addition, the gap between the flights of immature and mature females in unmanaged sweet or tart cherry orchards was shorter than in managed orchards or black cherry tree sites. We also determined fruit color, size, and skin hardness to characterize the progression of fruit maturity. We found that fruit became mature earlier in sweet and tart cherry orchards than in black cherry tree sites. This study indicates that the timing of female reproductive maturity is plastic and varies among cherry fruit fly populations present in distinct habitats. Variation in the timing of reproductive maturity is related to the fruit maturity period of distinct host plant species and to orchard management.

  9. Analysis of Agromorphological Descriptors to Differentiate between Duke Cherry (Prunus x gondouinii (Poit. & TurpinRehd. and Its Progenitors: Sweet Cherry (Prunus avium L. and Sour Cherry (Prunus cerasus L. Análisis de Descriptores Agromorfológicos para Diferenciar entre Cerezo Duke (Prunus x gondouinii (Poit. & Turpin Rehd. y sus Progenitores: Cerezo (Prunus avium L. y Guindo (Prunus cerasus L.

    Directory of Open Access Journals (Sweden)

    Rodrigo Pérez

    2010-03-01

    Full Text Available The rapid identification of the hybrids between sweet cherry (Prunus avium L. and sour cherry (Prunus cerasus L. is not easy. In order to resolve this problem, 18 Spanish sweet, sour and duke cherry cultivars were surveyed and characterized using 43 agromorphological descriptors evaluated in flowers, leaves, dormant 1-yr-old shoots, fruits, and trees during 2005 and 2006. Based on quantitative parameters, ANOVA and stepwise discriminant analysis (SDA were carried out. For qualitative descriptors, statistical comparisons were done by means of the chi-square (χ2 test. As result of the study, two quantitative (titratable acidity and number of lenticels and six qualitative descriptors (shape of the central and lateral lobes in the internal bracts of the flower fascicles, leaf shape and margin, pubescence in the veins of the lower side of the leaf, and type of sulci of the seed coat were identified as differential parameters in P. avium, P. cerasus and P. x gondouinii(Poit. & Turpin Rehd. Also, another four qualitative descriptors (petal coloration at the end of blooming, leaf stipule type, and seed shape and viability were found to be useful for easy differentiation between sour and duke cherry. None of these parameters has been employed previously to discriminate among sweet, sour and duke cherry.Los híbridos de cerezo (Prunus avium L. y guindo (Prunus cerasus L. no son fáciles de identificar. Para resolver este problema, 18 cultivares de cerezo, guindo y sus híbridos fueron prospectados y caracterizados agromorfológicamente mediante el estudio de 43 descriptores evaluados en flores, hojas, frutos, ramas de 1 año y árbol durante los años 2005 y 2006. En base a los resultados obtenidos del estudio de los diferentes parámetros cuantitativos se realizaron un ANDEVA y un análisis discriminante escalonado (SDA. Los descriptores cualitativos fueron analizados mediante el test de Chi-cuadrado (χ². Como resultado del estudio se identificaron

  10. Management of genetic resources in the nursery system of wild cherry (Prunus avium L.

    Directory of Open Access Journals (Sweden)

    Proietti R

    2006-01-01

    Full Text Available Knowledge of genetic and adaptive traits of reproductive materials used in the nursery system of wild cherry, could be an useful instrument to improve ecological and economic sustainability of plantation ecosystems. This work reports results from a research which the objectives were: 1 to study the genetic variation of a Prunus avium L. Population, used for seed harvesting, through its multi-locus genotypes detected by starch gel electrophoresis; 2 to analyze the level of genetic variation within and among different steps in a commercial nursery system (basic population and sub-populations, seedlings aged S1T1 and S1T2, plantation. Results showed low genetic variation levels of the basic population, similar to a reference system of other 12 wild cherry Italian populations and to other French and Caucasian materials. The genetic distances among Monte Baldo and some closer Lombardy provenances (Area Garda, Bosco Fontana, Valtellina were smaller than the Venice Region populations (Monti Lessini and Asiago. Number of alleles and percentage of polymorphic loci within the complex of Monte Baldo provenance and multiplication materials were similar, whilst a variable value of Fis was noted. Indeed, along with the nursery system until the plantation, heterozygosis initially (S1T1 increased, then decreased proceeding to the plantation. This fluctuation of FIS values could be determined by seed lots characterized initially by higher levels of variation, due to self-incompatibility. In the following steps, a possible selection pressure can affect randomly the genotypic structure of wild cherry by increasing the homozygosity. There is not among population a well defined geographic characterization, as suggested by genetic distances, therefore homogeneous seed harvest could be established an area larger than geographic and administrative borders. On this way we could have reproductive material with a wide genetic base and environmental adaptability. To

  11. Agronomic properties of wastewater sludge biochar and bioavailability of metals in production of cherry tomato (Lycopersicon esculentum).

    Science.gov (United States)

    Hossain, Mustafa K; Strezov, Vladimir; Chan, K Yin; Nelson, Peter F

    2010-02-01

    This work presents agronomic values of a biochar produced from wastewater sludge through pyrolysis at a temperature of 550 degrees C. In order to investigate and quantify effects of wastewater sludge biochar on soil quality, growth, yield and bioavailability of metals in cherry tomatoes, pot experiments were carried out in a temperature controlled environment and under four different treatments consisting of control soil, soil with biochar; soil with biochar and fertiliser, and soil with fertiliser only. The soil used was chromosol and the applied wastewater sludge biochar was 10tha(-1). The results showed that the application of biochar improves the production of cherry tomatoes by 64% above the control soil conditions. The ability of biochar to increase the yield was attributed to the combined effect of increased nutrient availability (P and N) and improved soil chemical conditions upon amendment. The yield of cherry tomato production was found to be at its maximum when biochar was applied in combination with the fertiliser. Application of biochar was also found to significantly increase the soil electrical conductivity as well as phosphorus and nitrogen contents. Bioavailability of metals present in the biochar was found to be below the Australian maximum permitted concentrations for food.

  12. 山东地区樱桃绿环斑驳病毒(CGRMV)的RT-PCR 检测及外壳蛋白基因的克隆%DETECTION OF CHERRY GREEN RING MOTTLE VIRUS (CGRMV) AND ISOLATION OF ITS COAT PROTEIN GENES FROM SWEET CHERRY IN SHANDONG PROVINCE

    Institute of Scientific and Technical Information of China (English)

    王文文; 宗晓娟; 陈立伟; 王甲威; 魏海蓉; 徐丽; 孟艳玲; 严雪瑞; 刘庆忠

    2012-01-01

    为调查我省甜樱桃感染樱桃绿环斑驳病毒(Cherry Green Ring Mottle Virus,CCRMV)情况,本研究以甜樱桃(Prunus avium L.)品种“红灯”叶片总RNA为模板,根据CGRMV基因组序列设计特异引物,对山东地区37份甜樱桃“红灯”样品进行RT - PCR检测,共检测出19份阳性样品.利用CGRMV外壳蛋白基因序列引物,从阳性植物样本中分离到约800bp的目的片段,克隆测序,序列分析显示该片段全长807 bp,编码268个氨基酸,与G enBank中已登录的CGRMV分离物的外壳蛋白基因序列一致性为87% ~97%,氨基酸序列相似性为95%~99%.该结果表明山东地区甜樱桃生产园中感染CGRMV的病例较为普遍.%In order to investigate the infection of Cherry Green Ring Mottle Virus (CGRMV) from sweet cherry ( Prunus avium L. ) in Shandong province, total RNA was extracted from the leaves of the sweet cherry cultivar Red Lamp' The specific primers were designed corresponding to the CGRMV genome sequence and used for the RT - PCR detection. Thirty - seven sweet cherry samples from six orchards were analyzed in the experiment and nineteen of them were detected as positive. The gene that encodes the virus coat protein was amplified and se-quenced. Sequence analysis revealed that the fragment was 807 nucleotides in length, encoding 268 amino acids. It shared 87% ~97% identity to the other CGRMV isolates reported in GenBank in the nucleotide level and 95% ~99% similarity in the amino acids level. These results showed CGRMV had been wide spread on sweet cherry in Shandong district.

  13. Genetic Diversity of European and Chinese Oilseed Brassica rapa Cultivars from Different Breeding Periods

    Institute of Scientific and Technical Information of China (English)

    ZHAO Yong-guo; Ofori Atta1; LU Chang-ming

    2009-01-01

    The Brassica oilseed crops went through two major breeding bottlenecks during the introgression of genes for zero erucic acid and low glucosinolate content, respectively, which may lead to reduced genetic biodiversity of the crop. This study investigates the impact of these bottlenecks on the genetic diversity within and across European and Chinese winter B. Rapa cultivars. We compared eight cultivars from Europe and China, representing three different seed qualities from three different breeding periods: (1) high erucic acid, high glucosinolates (++); (2) zero erucic acid, high glucosinolates (0+); (3) zero erueic acid, low glueosonolates (00, canola quality). Diversity was estimated on 32 plants per cultivar, with 16 simple sequence repeat (SSR) markers covering each of the B. Rapa linkage groups. The analysis of molecular variance (AMOVA) showed that genetic variations within cultivars, across cultivars and across regions (Europe and China) were significant, with about 60% of the total variation within cultivars. There was a slight, but non-significant loss in genetic diversity within cultivars when comparing the three breeding periods as indicated by effective number of alleles (2.39,2.23, and 1.99 for breeding periods 1, 2, and 3, respectively), Shannon information index (0.93, 0.90, 0.75), and expected heterozygosity (0.51, 0.49, 0.42). By cluster analysis (UPGMA dendrogram) and principal coordinate analysis, Chinese and European cultivars were clearly divided into two distinct groups. In conclusion, quality improvement did not significantly reduce the genetic diversity of European and Chinese B. Rapa cultivars.

  14. PHENOTYPIC CORRELATIONS AMONG WOOD PROPERTIES AND GROWTH IN WILD CHERRY PLANTATIONS

    Directory of Open Access Journals (Sweden)

    Michela Nocetti,

    2012-06-01

    Full Text Available Italy is one of the major users of cherry wood (Prunus avium L., and its cultivation has been increasing since the early nineties, together with studies aimed at improving genotypes, with a selection usually based on growth, stem form, vigor, and tree adaptation. Here, the phenotypic correlations between growth rate and the physical and mechanical properties of wood are investigated in two wild cherry plantations. X-ray microdensitometry was also applied to analyze the age-age correlations of wood density. It was shown that growth rate did not affect wood properties, and density was confirmed to be the trait that best represents the main properties of wood. The absence of relationships between wood density and growth rate was confirmed by densitometric analysis, although an increase of wood density with an increase of ring width was detected for very narrow rings (less than 3 mm wide. The average density at age 7 to 8 was observed to be highly correlated (r > 0.90 to the average density of a 19-year-old tree, allowing reliable estimation of the wood properties of older trees by early investigation of young trees.

  15. Yield of cherry tomatoes as a function of water salinity and irrigation frequency

    Directory of Open Access Journals (Sweden)

    Alexandre N. Santos

    2016-02-01

    Full Text Available ABSTRACT The use of brackish water in agriculture can cause salinization of soils and reduce plant yield. This problem can be minimized by hydroponic cultivation, which improves plant development. The aim of this study was to evaluate the yield of cherry tomatoes grown in hydroponic system with substrate under salinity levels of the nutrient solution (NS, exposure time to salinity and irrigation frequency. The experiment was conducted in a greenhouse, in a randomized complete block design, in a 6 x 2 x 2 factorial scheme with five replicates: six salinity levels of NS prepared with brackish water (3.01; 4.51; 5.94; 7.34; 8.71 and 10.40 dS m-1; two exposure times to NS (60 and 105 days and two irrigation frequencies (one irrigation per day and irrigation every two days. Yield and production components of cherry tomatoes cv. 'Rita' were evaluated. NS salinity affected plant yield, reducing fruit production, which was more significant when plants were subjected to a longer time of exposure to salinity. There was no difference between NS applications on fruit production, when these applications were performed once a day or once every two days.

  16. I. V. Michurin’S Work on Expansion of the Plant Horticulture Assortment and Improvement of Food Quality

    Directory of Open Access Journals (Sweden)

    Sokolov Viktor V.

    2015-09-01

    Full Text Available October 2015 marks the 160th anniversary of the birth of Ivan V. Michurin. As a scientist and plant breeder, he made a significant improvement of many fruit and berry plants, and flowers. He developed methods of plant breeding, especially regarding long-distance hybridisation of fruit plants, and promoted gardening to the north and east of Russia. He introduced some new berry species, such as Actinidia and black chokeberry, and was the first in Russia to use dwarf and semi dwarf stocks of apples. Michurin initiated the mass movement of gardeners and horticulture experimenters in USSR who changed and significantly extended the assortment and areas of fruit and berries cultivation in the country. He not only brought together a representative collection of species and varieties of fruit, berry and flower plants from around the world, but also used them in breeding by hybridization, including interspecific. He created some new artificial interspecific hybrids such as Cerapadus (cherry and bird cherry tree hybrid, and others. Michurin created 132 cultivars. Eleven of them are not only cultivated, but are also included in “The State Register of Protected Plant Breeding of the Russian Federation”.

  17. Evaluation of Drought Tolerance in Some of Winter Canola Cultivars

    Directory of Open Access Journals (Sweden)

    J Rashidifar

    2012-10-01

    Full Text Available In order to evaluate terminal drought tolerance in different Canola cultivars based on drought tolerance indices, an experiment was conducted based on randomized complete block design (RCBD with three replications at experimental field of Seed and Plant Improvement Institute (SPII, Karaj in 2008-09. Results using stress tolerance index (STI, geometric mean productivity (GMP and mean productivity (MP indices revealed Zarfam, Talaye and GKH305, were as cultivars with highest yield in both optimum irrigation and terminal drought conditions. High and positive correlation between STI, GMP and MP indices with yield in optimum and drought conditions indicated that they were as the best indices for introduction and characterization of drought tolerant canola cultivars. Biplot graph showed that MP, GMP and STI indices had high correlation coefficient with each other, and tolerant genotypes were located near tolerance indices. Also results of three dimensional scatter plot indicated that cultivars Zarfam, GKH 305 and Tassilo were recognized as tolerant genotypes to drought stress, and cultivars ES Astrid, GK Helena, Modena and Okapi were identified as sensitive genotypes.

  18. Chemical characteristics and volatile profile of genetically modified peanut cultivars.

    Science.gov (United States)

    Ng, Ee Chin; Dunford, Nurhan T; Chenault, Kelly

    2008-10-01

    Genetic engineering has been used to modify peanut cultivars for improving agronomic performance and pest resistance. Food products developed through genetic engineering have to be assessed for their safety before approval for human consumption. Preservation of desirable chemical, flavor and aroma attributes of the peanut cultivars during the genetic modifications is critical for acceptance of genetically modified peanuts (GMP) by the food industry. Hence, the main objective of this study is to examine chemical characteristics and volatile profile of GMP. The genetically modified peanut cultivars, 188, 540 and 654 were obtained from the USDA-ARS in Stillwater, Oklahoma. The peanut variety Okrun was examined as a control. The volatile analysis was performed using a gas chromatograph/mass spectrometer (GC/MS) equipped with an olfactory detector. The peanut samples were also analyzed for their moisture, ash, protein, sugar and oil compositions. Experimental results showed that the variations in nutritional composition of peanut lines examined in this study were within the values reported for existing cultivars. There were minor differences in volatile profile among the samples. The implication of this study is significant, since it shows that peanut cultivars with greater pest and fungal resistance were successfully developed without major changes in their chemical characteristics.

  19. Analysis of pecan cultivars Mahan and Western in East China.

    Science.gov (United States)

    Luo, X; Li, Z; Sun, Z; Wan, X

    2016-09-16

    Pecan (Carya illinoensis) has been introduced to East China for over one hundred years, but its planting is still only occurring at a small scale. The key limiting factor is its low yield. To enhance the yield pecan in East China, two pecan cultivars, Mahan and Western, were examined. Twenty traits describing phasic development, yield, nut quality, and cultural practice were investigated. We found that pecan cultivar Mahan gives a higher yield and nut quality than cultivar Western. We recommend interplanting of cultivar Pawnee to act as a pollinator tree. Appropriate cultivation practices that can be implemented to enhance fruit yield of cultivars Mahan and Western include soil-applied paclobutrazol (PBZ) at certain concentrations, pinching, and supplementary pollination. For example, the addition of 1.25 g/m(2) of PBZ inhibits pecan branch growth and stimulates short bearing branches, which promotes fruit yield. We found that soil-applied PBZ reached optimal performance 82 days after application. A pinching length of 40 cm resulted in a fruit yield increase. In addition, grafting and transplantation may promote male flowering, but delays female flowering. These cultural practices may provide insights that can be used to improve pecan cultivation in East China.

  20. Apple tree production in Italy: rootstocks, cultivars, fertilization, and irrigation

    Directory of Open Access Journals (Sweden)

    Giovambattista Sorrenti

    2012-11-01

    Full Text Available Italy is one of the main apple producers in Europe, primarily intended for fresh consumption, both in the domestic and foreign markets. Fruit yield and quality depends on the cultivar, rootstock, and management practices, such as the fertilization and irrigation adopted in the orchard. This review aims at reporting the main apple cultivars and rootstocks, the management of fertilization and irrigation, as well as their adaptation to apple tree orchards in Italy. The programs for genetic improvement carried out in this country involved the selection of apple tree cultivars and rootstocks which enable a high fruit yield and quality, in order to meet the requirements from the consumer market. In the fertilization and irrigation management, nutrients and water are supplied in amounts next to the actual need of the plants, providing an adequate nutrition, a satisfactory yield, and high quality fruits, besides preventing, whenever possible, nutrients and water losses in the environment.

  1. Development of ochratoxin A during robusta (Coffea canephora) coffee cherry drying.

    Science.gov (United States)

    Bucheli, P; Kanchanomai, C; Meyer, I; Pittet, A

    2000-04-01

    The occurrence and formation of ochratoxin A (OTA) in Robusta coffee was studied for three consecutive seasons under tropical conditions in Thailand. Sun drying of coffee cherries consistently led to OTA formation in the pulp and parchment (husks) of the cherries. In replicated trials, dried coffee beans (green coffee) were shown to contain on average OTA concentrations that were approximately 1% of those found in husks. OTA contamination of green coffee depended on cherry maturity, with green cherries being the least, and overripe cherries the most susceptible. Defects, and in particular the inclusion of husks, are the most important source of OTA contamination. OTA contamination occurred independently of whether cherries were placed on concrete, on bamboo tables, or on the ground. The study suggests that better raw material quality, an appropriate drying and dehulling procedure combined with a reduction of green coffee defects can effectively contribute to the reduction of OTA in green coffee.

  2. 大樱桃果蝇无公害防治技术浅析%Pollution-free Prevention and Control Technology of Cherry Fruit Fly

    Institute of Scientific and Technical Information of China (English)

    张小花; 侯攻科; 李琳娜

    2015-01-01

    Along with the increasing of the cultivated area and the increase of introduction quantity in the field, the kinds of pests and diseases of the great cherry were on the rise. In order to effectively prevent and control fruit fly, improve the yield and quality of cherry, the big cherry industry in Qinzhou district sustainable development, this paper simply introduces the control measures of the cherry fruit flies.%随着栽培面积的不断扩大和在外地引种量的增加,为害大樱桃的病虫害种类呈上升趋势,为了有效的预防和控制果蝇,提高大樱桃的产量和质量,使秦州区大樱桃产业健康可持续发展,本文简单介绍了几点大樱桃果蝇的防治措施。

  3. IPR 118 - Bread wheat cultivar

    Directory of Open Access Journals (Sweden)

    Carlos Roberto Riede

    2007-01-01

    Full Text Available Wheat cultivar IPR 118 developed by IAPAR has a good yield potential and is widely adapted. It is earlymaturing and moderately tolerant to shattering and soil aluminum, moderately resistant to leaf rust and presents high glutenstrength for bread-making. The overall yield exceeded controls by 13%.

  4. THE REAL OPTIONS PUZZLE FOR MICHIGAN TART CHERRY PRODUCERS

    OpenAIRE

    Nyambane, Gerald G.; Black, J. Roy

    2004-01-01

    Capital budgeting decisions faced by tart cherry producers often challenge our traditional valuation techniques. Real Options Valuation (ROV) methods may be useful but assumptions of existing ROV approaches are restrictive and, in some cases, unrealistic. In this paper we assert that use of existing option pricing methods can not be justified. Instead, dynamic programming approach is more appropriate. We develop a multi-period model and use it to obtain an optimal orchard replacement policy. ...

  5. Establishment and early development of 'Kanza', 'Peruque', and other pecan cultivars in northern U.S. growing regions

    Science.gov (United States)

    Most pecan (Carya illinoensis) nut production in the northern range of the species (Missouri, Kansas, Northern Arkansas) is from managed wild trees. Orchards of trees grafted to improved cultivars are slowly being established in the region as economic opportunities improve. Pecan cultivars that are ...

  6. Genetic base of Brazilian irrigated rice cultivars

    Directory of Open Access Journals (Sweden)

    Hudson de Oliveira Rabelo

    2015-08-01

    Full Text Available The aim of this study was to estimate the genetic base of Brazilian irrigated rice cultivars released in the period from 1965 to 2012. The genealogies of the cultivars were obtained based on information from marketing folders, websites, crossings records, and scientific articles. The following factors were calculated: relative genetic contribution (RGC, accumulated genetic contribution (AGC, frequency (in percentage of each ancestor in the genealogy (FAG, number of ancestors that constitute each cultivar (NAC,number of ancestors responsible for 60%, 70%, 80% and 90% of the genetic base (NAGB, and average number of ancestor per cultivar (ANAC. The cultivars were also grouped based on the period of release (1965-1980, 1981-1990, 1991-2000 and 2001-2012. For each grouping, the previously described factors were also estimated. A total of 110 cultivars were studied and it was concluded that the genetic base of Brazilian irrigated rice cultivars is narrow.

  7. Evaluation of Drought Resistance Indicates for Yield and Its Components in Three Triticale Cultivars

    Directory of Open Access Journals (Sweden)

    G. Kinaci

    2010-05-01

    Full Text Available Drought is a wide-spread problem seriously influencing cereal production and quality. The development of triticale cultivars which are tolerant to drought is an objective in many breeding programmes, but so far success has been limited. This study was carried to examine differences in yield and yield components and kernel features among triticale cultivars (Tatlicak 97, Karma 2000 and MIKHAM 2002 under drought stress. Three triticale cultivars with different yield performance were grown in separate experiments under the rain fed and irrigated conditions at Eskisehir, Turkey, in 2006-2007 growing season. In the study, susceptibility index (SSI, stress tolerance index (STI, tolerance (TOL, yield index (YI, yield stability index (YSI, mean productivity (MP and geometric mean productivity (GMP were calculated. The best yielding cultivar under the drought stress, hence having a low susceptibility index, was Karma 2000. This cultivar may be utilized for improvement of drought resistance in triticale breeding programmes.

  8. Cultivar variation and selection potential relevant to the production of cellulosic ethanol from wheat straw

    DEFF Research Database (Denmark)

    Lindedam, Jane; Andersen, Sven Bode; DeMartini, J.;

    2012-01-01

    -plate technique. This technique enabled us to estimate cultivar-related and environmental correlations between sugar yield, chemical composition, agronomic qualities, and distribution of botanical plant parts of wheat straw cultivars. Straws from 20 cultivars were collected in duplicates on two sites in Denmark......Optimizing cellulosic ethanol yield depends strongly on understanding the biological variation of feedstocks. Our objective was to study variation in capacity for producing fermentable sugars from straw of winter wheat cultivars with a high-throughput pretreatment and hydrolysis well...... suggest that selection of cultivars for improved biofuel feedstock of wheat straw is possible, because heritability of sugar release is 57% and there are few adverse correlations to other agronomic traits....

  9. Breeding of high yield, double low and disease resistant rapeseed cultivars by population improvement%应用群体改良法选育高产优质抗病油菜新品种

    Institute of Scientific and Technical Information of China (English)

    陆光远; 张学昆; 李桂英; 程勇; 郑普英; 瞿桢; 付桂萍; 邹崇顺

    2012-01-01

    为了探究轮回选择在油菜品种群体改良中的作用,利用具有高产、双低、抗病等优异性状的种质材料为育种亲本,以显性细胞核雄性不育基因为桥梁,构建了一个随机交配轮回选择群体.针对群体的产量、品质、抗病性等性状进行了多次轮回选择,结果表明经过6轮选择后,群体的主要性状得到改良,产量增加11.3%,每轮遗传增益为1.88%;芥酸和硫苷含量每轮遗传增益达到10.12%和6.37%,改良效果明显;但菌核病的抗性改良效果不明显.经过多轮选择后群体的遗传离散度仍然较高(0.338 3 ~0.377 7),表明遗传多样性并没有明显下降.从不同轮次群体中选择优异单株,经系谱选择后育成了中双5号、中双6号和中双10号等优良品种,在生产上应用广泛,表明轮回选择在油菜品种改良方面具有巨大潜力.%To study the effectiveness of recurrent selection in population improvement of rapeseed variety, a recurrent selection population was constructed by mixing several germplasm populations with high - yield, double -low and disease - resistant characteristics, using dominant genie male sterile line as a bridge for random mating. This recurrent population was then subjected to consecutive selections mainly on yield, quality and Sclerotinia stem rot resistance. Results showed that, after 6 selection cycles on the population, seed yield increased by 11. 3% with a genetic gain of 1. 88% per cycle. Except for stem rot resistance, obvious genetic improvements were found in eru-cic acid and glucosinolates contents, with a respective genetic gain of 10. 12% and 6. 37% . The population genetic dispersion was stable and high (from 0. 338 3 to 0. 377 7) , indicating no obvious penalty on genetic diversity after several cycles of selection. Ideal fertile plants identified in different generations were further developed into breeding lines by pedigree selection method. A number of new cultivars (i

  10. 'Gloriosa': cultivar de alface americana tropicalizada 'Gloriosa': crisphead tropicalized cultivar

    Directory of Open Access Journals (Sweden)

    Fernando C. Sala

    2008-09-01

    Full Text Available 'Gloriosa' é uma cultivar de alface (Lactuca sativa L. americana tropicalizada e resistente a Thielaviopsis basicola. Foi selecionada a partir de variantes da cultivar Lucy Brown, pelo método genealógico. Progênies S1 a S6 foram selecionadas para resistência a T. basicola, características hortícolas, tolerância ao pendoamento precoce e adaptação ao cultivo de verão. Apresenta ciclo precoce de 40 a 50 dias pós-transplante, pendoamento lento, cabeças grandes e três camadas de folhas externas. Possui tolerância a Xanthomonas campestris pv. vitians e suporta o cultivo nos períodos de elevadas temperaturas e pluviosidade. Trata-se da primeira cultivar de alface americana tropicalizada no Brasil.'Gloriosa' is a tropicalized crisphead lettuce (Lactuca sativa L. resistant to Thielaviopsis basicola. It was derived by selection from cv. Lucy Brown variants by pedigree method. Progenies S1 to S6 were selected for T. basicola resistance, desirable horticulture traits, slowbolting and adaptation for Brazilian summer crop. It presents a precocious cycle within 40 to 50 days of being planted, slowbolting, large head and three layers of frame levels. It is tolerant to Xanthomonas campestris pv. vitians and is able to be grown under high temperature and rainy summer in southern of Brazil. It is the first crisphead lettuce type developed for tropical regions of Brazil.

  11. Host susceptibility of citrus cultivars to Queensland fruit fly (Diptera: Tephritidae).

    Science.gov (United States)

    Lloyd, A C; Hamacek, E L; Smith, D; Kopittke, R A; Gu, H

    2013-04-01

    Citrus crops are considered to be relatively poor hosts for Queensland fruit fly, Bactrocera tryoni (Froggatt), as for other tephritid species. Australian citrus growers and crop consultants have reported observable differences in susceptibility of different citrus cultivars under commercial growing conditions. In this study we conducted laboratory tests and field surveys to determine susceptibility to B. tryoni of six citrus cultivars [(Eureka lemon (Citrus limon (L.) Osbeck); Navel and Valencia oranges (C. sinensis (L.) Osbeck); and Imperial, Ellendale, and Murcott mandarins (C. reticulata Blanco). The host susceptibility of these citrus cultivars was quantified by a Host Susceptibility Index, which is defined as the number of adult flies produced per gram of fruit infested at a calculated rate of one egg per gram of fruit. The HSI was ranked as Murcott (0.083) > Imperial (0.052) > Navel (0.026) - Ellendale (0.020) > Valencia (0.008) > Eureka (yellow) (0.002) > Eureka (green) (0). Results of the laboratory study were in agreement with the level of field infestation in the four citrus cultivars (Eureka lemon, Imperial, Ellendale, and Murcott mandarins) that were surveyed from commercial orchards under baiting treatments against fruit flies in the Central Burnett district of Queensland. Field surveys of citrus hosts from the habitats not subject to fruit fly management showed that the numbers of fruit flies produced per gram of fruit were much lower, compared with the more susceptible noncitrus hosts, such as guava (Psidium guajava L.), cherry guava (P. littorale Raddi), mulberry (Morus nigra L.), loquat (Eriobotrya japonica (Thunb.) Lindl.), and pear (Pyrus communis L.). Therefore, the major citrus crops commercially cultivated in Australia have a relatively low susceptibility to B. tryoni, with Eureka lemons being a particularly poor host for this tephritid fruit fly.

  12. Soil covering in organic cultivation of onion cultivars

    Directory of Open Access Journals (Sweden)

    Leonardo Barreto Tavella

    2015-03-01

    Full Text Available The soil preparation in horticulture, including organic, is characterized by intense soil tillage, which increases energy costs and unbalanced the environment. The organic system in onion cultivation has shown satisfactory results, however the soil covering use tends to improve the cultivation environment and may result in higher yields. The objective of this work was to evaluate the performance of onion cultivars in organic cultivation under different soil coverages. The experiment was conducted in the period of April to October of 2009, under protected cropping, in the experimental area of the horticulture sector of the Universidade Federal do Acre (UFAC, in Rio Branco, Acre, Brazil. A randomized block design was adopted, in a split-plot arrangement, the plots being comprised of soil coverings: coffee bean husks, grass straw (Brachiaria decumbens; dessicated bamboo leaves (Bambusa spp. and uncovered soil (control treatment, and the subplots comprised of three onion cultivars (IPA 10, IPA 11 and IPA 12, with four repetitions. The variables analyzed were total bulb yield (t ha-1, marketable bulb yield (t ha-1, fresh mass of the bulb (g bulb-1, classification of the bulbs and loss of mass as a function of storage time. There was no interaction effect between the soil coverings and the cultivars. Cultivars IPA 10 and IPA 11 showed greater agronomic performance of the studied variables. The soil coverings did not affect the yield and average mass of the bulbs under organic cultivation. The three cultivars presented more than approximately 70% of the bulbs in class 2 and a maximum of 5% of unmarketable bulbs. The loss of mass after 49 days of storage was 10% independent of the cultivar or soil covering.

  13. Comparison of the genetic determinism of two key phenological traits, flowering and maturity dates, in three Prunus species: peach, apricot and sweet cherry.

    Science.gov (United States)

    Dirlewanger, E; Quero-García, J; Le Dantec, L; Lambert, P; Ruiz, D; Dondini, L; Illa, E; Quilot-Turion, B; Audergon, J-M; Tartarini, S; Letourmy, P; Arús, P

    2012-11-01

    The present study investigates the genetic determinism of flowering and maturity dates, two traits highly affected by global climate change. Flowering and maturity dates were evaluated on five progenies from three Prunus species, peach, apricot and sweet cherry, during 3-8 years. Quantitative trait locus (QTL) detection was performed separately for each year and also by integrating data from all years together. High heritability estimates were obtained for flowering and maturity dates. Several QTLs for flowering and maturity dates were highly stable, detected each year of evaluation, suggesting that they were not affected by climatic variations. For flowering date, major QTLs were detected on linkage groups (LG) 4 for apricot and sweet cherry and on LG6 for peach. QTLs were identified on LG2, LG3, LG4 and LG7 for the three species. For maturity date, a major QTL was detected on LG4 in the three species. Using the peach genome sequence data, candidate genes underlying the major QTLs on LG4 and LG6 were investigated and key genes were identified. Our results provide a basis for the identification of genes involved in flowering and maturity dates that could be used to develop cultivar ideotypes adapted to future climatic conditions.

  14. Oral immunization of animals with transgenic cherry tomatillo expressing HBsAg

    Institute of Scientific and Technical Information of China (English)

    Yi Gao; Yina Ma; Mei Li; Tonq Chenq; Shao-Wei Li; Jun Zhang; Ning-Shao Xia

    2003-01-01

    AIM: To investigate the expression of recombinant HBsAg (rHBsAg) in transgenic cherry tomatillo in order to explore the feasibility of producing HBV oral vaccine with cherry tomatillo by animal immune tests.METHODS: The recombinant plant expression vector containing HBsAg gene was constructed. Mediated with Agrobacterium tumefaciens, HBsAg gene was transferred into cotyledons of cherry tomatillo. Transformed cherry tomatillos were obtained through hygromycin delay-selection. Integrated DNA in transgenic cherry tomatillo was confirmed by hygromycin resistance selection, Gus detection, polymerase chain reaction (PCR) and dot blotting analysis. Antigenicity of rHBsAg was examined by ELISA and the immunogenicity of rHBsAg derived from transgenic cherry tomatillo tissues was confirmed by oral feed of transformed tissues to BALB/c mice primed with commercial HBV vaccines. Specific antibody titers in mice's serum were examined by ELISA every week.RESULTS: By far, 10 positive lines of transgenic cherry tomatillos containing HBsAg gene were obtained. Among different organs of the same transgenic cherry tomatillo,level of rHBsAg expressed in leaves was the highest with the yield up to 300ng/g fresh weight. And the rHBsAg expression level in fruits was about 10 ng/g fresh weight.In animal immune tests, oral delivery with transgenic tissues to mice primed with commercial vaccine instead of naive mice resulted in significant immune response.CONCLUSION: The result of this animal immune test indicated the rHBsAg derived from transgenic cherry tomatillo possessed normal immunogenicity. This work demonstrated the feasibility to generate oral immunogenic rHBsAg in transgenic cherry tomatillo, and would provide some experimental approach for the production of low-cost oral vaccines using transgenic cherry tomatillo in large scale.

  15. Cultivar de soja BRS 205

    Directory of Open Access Journals (Sweden)

    Bonato Emídio Rizzo

    2002-01-01

    Full Text Available A BRS 205, cultivar de soja de ciclo semiprecoce, obtida do cruzamento [BR-16(2 x Ocepar 8] x Tracy-M, é indicada para o Rio Grande do Sul, em semeaduras de novembro. Possui crescimento determinado, plantas de flor branca, pubescência marrom, porte baixo e grãos de hilo preto. Tem resistência ao cancro-da-haste (Diaporthe phaseolorum f. sp. meridionalis, à podridão-parda-da-haste (Phialophora gregata, à mancha-olho-de-rã (Cercospora sojina e à pústula bacteriana (Xanthomonas axonopodis pv. glycines. É moderadamente resistente ao oídio (Microsphaera diffusa. O rendimento médio de grãos foi 6% e 11% superior ao das cultivares RS 7-Jacuí e BR-16, respectivamente.

  16. Microsporogenesis of Apricot Cultivars in Hungary

    Directory of Open Access Journals (Sweden)

    Veronika HAJNAL

    2013-12-01

    Full Text Available Like all phenological processes, microsporogenesis is mainly determined genetically, but its phenotypical expression is greatly influenced by environmental factors, particularly the temperature. During the 3-year experimental period, the process of microsporogenesis was examined in eight apricot cultivars originated in North America and in Romania. The Hungarian cultivar ‘Gönci magyar kajszi’ was used as the control. Based on the results it was possible to rank the cultivars in terms of their microsporogenesis schedule. The same order was found in all three years. This order also indicates the yield reliability of the cultivars. Endodormancy ended between January 10th and 25th in the buds of the earliest cultivar ‘Pinkcot’, but not until February 5-10th in the latest cultivar ‘Harlayne’. Three of the cultivars had faster flower bud development than the control, in the order ‘Pinkcot’, ‘Orange Red’ and ‘Harcot’. Growing these cultivars thus involves greater risk than for ‘Gönci magyar kajszi’ in Hungary, due to their rapid winter flower bud development. Some cultivars found to have slower flower bud development than ‘Gönci magyar kajszi’; ‘Litoral’, ‘Harogem’, ‘Comandor’, ‘Sirena’ and ‘Harlayne’ – thus these cultivars can be grown more reliably.

  17. 33 CFR 334.430 - Neuse River and tributaries at Marine Corps Air Station Cherry Point, North Carolina; restricted...

    Science.gov (United States)

    2010-07-01

    ... Marine Corps Air Station Cherry Point, North Carolina; restricted area and danger zone. 334.430 Section... Air Station Cherry Point, North Carolina; restricted area and danger zone. (a) The restricted area... Station, Cherry Point, North Carolina, extending from the mouth of Hancock Creek to a point...

  18. 76 FR 31577 - Notice of Availability of a Pest Risk Analysis for the Importation of Fresh Apricot, Sweet Cherry...

    Science.gov (United States)

    2011-06-01

    ... Importation of Fresh Apricot, Sweet Cherry, and Plumcot Fruit From South Africa Into the Continental United... importation into the continental United States of fresh apricot, sweet cherry, and plumcot fruit from South... (Prunus armeniaca L.), sweet cherry (Prunus avium (L.) L., and plumcot (Prunus domestica x...

  19. 78 FR 25407 - Safety Zones; National Cherry Festival Air Show and Fireworks Display; West Grand Traverse Bay...

    Science.gov (United States)

    2013-05-01

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zones; National Cherry Festival Air Show and..., the National Cherry Festival in Traverse City, MI will host an air show over the West Arm of Grand Traverse Bay. At the conclusion of the National Cherry Festival on July 6, 2013, fireworks will be...

  20. Impact of prolonged absence of low temperature on adult eclosion patterns of western cherry fruit fly (Diptera: Tephritidae)

    Science.gov (United States)

    The western cherry fruit fly, Rhagoletis indifferens (Curran) (Diptera: Tephritidae), is a serious pest of cherries (Prunus spp.) in the Pacific Northwest of the U.S.A. Previous research suggests that R. indifferens is unlikely to establish in commercial cherry production areas in California and in ...

  1. 77 FR 24640 - Tart Cherries Grown in the State of Michigan, et al.; Increasing the Primary Reserve Capacity and...

    Science.gov (United States)

    2012-04-25

    ...; ] DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in the State of... diversion activities prescribed under the marketing order for tart cherries (order). The order regulates the handling of tart cherries grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah,...

  2. 77 FR 33303 - Tart Cherries Grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah, Washington...

    Science.gov (United States)

    2012-06-06

    ... Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, New York.... 930 (order), which regulates the handling of tart cherries grown in Michigan, New York, Pennsylvania... (77 FR 13015). This document directed that a referendum among tart cherry growers and processors...

  3. 75 FR 29647 - Tart Cherries Grown in the States of Michigan, et al.; Final Free and Restricted Percentages for...

    Science.gov (United States)

    2010-05-27

    ... Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, et al.; Final Free and Restricted... Federal marketing order regulating tart cherries grown in seven States (order). The percentages are 32... tart cherries grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah, Washington,...

  4. Processed tart cherry products--comparative phytochemical content, in vitro antioxidant capacity and in vitro anti-inflammatory activity.

    Science.gov (United States)

    Ou, Boxin; Bosak, Kristen N; Brickner, Paula R; Iezzoni, Dominic G; Seymour, E Mitchell

    2012-05-01

    Processing of fruits and vegetables affects their phytochemical and nutrient content. Tart cherries are commercially promoted to possess antioxidant and anti-inflammatory activity. However, processing affects their phytochemical content and may affect their related health benefits. The current study compares the in vitro antioxidant capacity and anti-inflammatory cyclooxygenase activity of processed tart cherry (Prunus cerasus) products-cherry juice concentrate, individually quick-frozen cherries, canned cherries, and dried cherries. Cherry products were analyzed for total anthocyanin and proanthocyanidin content and profile. On a per serving basis, total anthocyanins were highest in frozen cherries and total proanthocyanidins were highest in juice concentrate. Total phenolics were highest in juice concentrate. Juice concentrate had the highest oxygen radical absorbance capacity (ORAC) and peroxynitrite radical averting capacity (NORAC). Dried cherries had the highest hydroxyl radical averting capacity (HORAC) and superoxide radical averting capacity (SORAC). Processed tart cherry products compared very favorably to the U.S. Dept. of Agriculture-reported ORAC of other fresh and processed fruits. Inhibition of in vitro inflammatory COX-1 activity was greatest in juice concentrate. In summary, all processed tart cherry products possessed antioxidant and anti-inflammatory activity, but processing differentially affected phytochemical content and in vitro bioactivity. On a per serving basis, juice concentrate was superior to other tart cherry products.

  5. 75 FR 29684 - Tart Cherries Grown in the States of Michigan, et al.; Increased Assessment Rate for the 2010...

    Science.gov (United States)

    2010-05-27

    ... Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, et al.; Increased Assessment Rate for the 2010-2011 Crop Year for Tart Cherries AGENCY: Agricultural Marketing Service, USDA... assessable tart cherries. The Board locally administers the marketing order which regulates the handling...

  6. 77 FR 36115 - Tart Cherries Grown in the States of Michigan, et al.; Final Free and Restricted Percentages for...

    Science.gov (United States)

    2012-06-18

    ... Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, et al.; Final Free and Restricted Percentages for the 2011-12 Crop Year for Tart Cherries AGENCY: Agricultural Marketing Service, USDA. ACTION... year under the marketing order for tart cherries grown in the states of Michigan, New...

  7. 75 FR 12702 - Tart Cherries Grown in the States of Michigan, et al.; Final Free and Restricted Percentages for...

    Science.gov (United States)

    2010-03-17

    ...; ] DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, et al.; Final Free and Restricted Percentages for the 2009-2010 Crop Year for Tart Cherries... order regulates the handling of tart cherries grown in the States of Michigan, New York,...

  8. 75 FR 33673 - Tart Cherries Grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah, Washington...

    Science.gov (United States)

    2010-06-15

    ... Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah... regulates the handling of tart cherries grown in Michigan, New York, Pennsylvania, Oregon, Utah, Washington... referendum among tart cherry growers and processors be conducted during the period February 1, 2010,...

  9. 75 FR 33736 - Tart Cherries Grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah, Washington...

    Science.gov (United States)

    2010-06-15

    ... Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, New York... regulates the handling of tart cherries grown in Michigan, New York, Pennsylvania, Oregon, Utah, Washington... amend Marketing Agreement and Order No. 930 (order) regulating the handling of tart cherries grown...

  10. 76 FR 10471 - Tart Cherries Grown in the States of Michigan, et al.; Final Free and Restricted Percentages for...

    Science.gov (United States)

    2011-02-25

    ... Marketing Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, et al.; Final Free and Restricted Percentages for the 2010-2011 Crop Year for Tart Cherries AGENCY: Agricultural Marketing Service... the tart cherry marketing order for the 2010-2011 crop year. The percentages are 58 percent free...

  11. 77 FR 12748 - Tart Cherries Grown in the States of Michigan, et al.; Final Free and Restricted Percentages for...

    Science.gov (United States)

    2012-03-02

    ...; ] DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, et al.; Final Free and Restricted Percentages for the 2011-12 Crop Year for Tart Cherries AGENCY... tart cherries grown in the states of Michigan, New York, Pennsylvania, Oregon, Utah, Washington,...

  12. 78 FR 46494 - Tart Cherries Grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah, Washington...

    Science.gov (United States)

    2013-08-01

    ... Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in the States of Michigan, New York... prescribed under the marketing order for tart cherries grown in the States of Michigan, New York... tart cherries grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah, Washington,...

  13. Sensing the Moisture Content of Dry Cherries - A Rapid and Nondestructive Method

    Science.gov (United States)

    Impedance (Z), and phase angle (') of a parallel-plate capacitor with a single cherry fruit between the plates was measured using a CI meter (Chari’s Impedance meter), at 1 and 9 MHz . Capacitance C, was derived from Z and ', and using the C, ', and Z values of a set of cherries whose moisture cont...

  14. Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus acidophilus

    NARCIS (Netherlands)

    Mohedano, M.L.; Garcia-Cayuela, T.; Perez-Ramos, A.; Gaiser, R.A.; Requena, T.; Lopez, P.

    2015-01-01

    Lactobacilli are widespread in natural environments and are increasingly being investigated as potential health modulators. In this study, we have adapted the broad-host-range vector pNZ8048 to express the mCherry protein (pRCR) to expand the usage of the mCherry protein for analysis of gene express

  15. Assessing the potential for establishment of western cherry fruit fly using ecological niche modeling

    Science.gov (United States)

    Sweet cherries, Prunus avium (L.) L., grown in the western United States are exported to many countries around the world. Some of these countries have enforced strict quarantine rules and trade restrictions due to concerns about the potential establishment and subsequent spread of western cherry fru...

  16. NIR detection of pits and pit fragments in fresh cherries (abstract)

    Science.gov (United States)

    The feasibility of using near infrared (NIR) diffuse reflectance spectroscopy for the detection of pits and pit fragments in cherries was demonstrated. For detection of whole pits, 300 cherries were obtained locally and pits were removed from half. NIR reflectance spectra were obtained in triplicate...

  17. Sour Cherry (Prunus cerasus L.) Anthocyanins: effects of juice processing on phenolic compounds and bioavailability

    NARCIS (Netherlands)

    Toydemir, G.; Boyacioglu, D.; Beekwilder, M.J.; Vos, de R.C.H.; Hall, R.D.; Capanoglu, E.

    2014-01-01

    Sour cherry (Prunus cerasus L.), has gained growing interest in recent years due to the envisaged health benefits associated with a regular intake of anthocyanins and related polyphenolic compounds. Turkish sour cherries are widely consumed as processed products and are renowned for their high juice

  18. Changes in sour cherry (Prunus cerasus L.) antioxidants during nectar processing and in vitro gastrointestinal digestion.

    NARCIS (Netherlands)

    Toydemir, G.; Capanoglu, E.; Kamiloglu, S.; Boyacioglu, D.; Vos, de C.H.; Hall, R.D.; Beekwilder, M.J.

    2013-01-01

    Sour cherry (Prunus cerasus L.) is rich in polyphenols, and like its processed products, is especially rich in anthocyanins. We have applied HPLC, spectrophotometric and on-line antioxidant detection methods to follow the fate of cherry antioxidants during an entire multi-step industrial-scale proce

  19. The Role of Top-down Attention in the Cocktail Party: Revisiting Cherry's Experiment after Sixty Years

    DEFF Research Database (Denmark)

    Marchegiani, Letizia; Karadogan, Seliz; Andersen, Taja;

    2011-01-01

    . Based on such simulations we expect that under strong top-down attention pattern recognition is improved as the model can compensate for noise and confounders. We next investigate the role of temporal and spectral overlaps and speech intelligibility in humans, and how the presence of a task influences...... their relation. For this purpose, we perform behavioral experiments inspired by Cherry's classic experiments carried out almost sixty years ago. We make participants listen to a mono signal consisting of two different narratives pronounced by a speech synthesizer under two different conditions. In the first case...

  20. Molecular Screening and Resistance Evaluation of American Wheat Cultivars to Chinese Stripe Rust Races

    Institute of Scientific and Technical Information of China (English)

    BAI Yu-lu; ZHANG Chun-yu; SUN Quan; LIN Feng; CUI Na; XU Shi-chang; GAO Yang; XU Xiao-dan

    2010-01-01

    Stripe rust,caused by Puccinia striiformis f.sp.tritici,is one of the major diseases of wheat in China.In order to asses the resistance levels and existing Yr genes among 59 wheat cultivars (lines) from the Pacific Northwest (PNW) of the United States,to provide resistance resources for genetic improvement of wheat stripe rust resistance in China,59 wheat cultivars (lines) from PNW of the United States were infected by 3 mixed races of predominant Chinese stripe rust races CRY31,CRY32,and CRY33 to evaluate their resistance at seedling and adult plant stages,and screened with molecular markers tightly linked to currently effective all-stage resistance genes Yr10,Yr15 and adult plant resistance genes Yr18,Yr39.Of 59 American cultivars (lines),five cultivars (lines),Expresso,02W50076,ACS52610,WA008012,and WA00801833,had all-stage resistance,showing resistance to mixed races of CRY31,CRY32,and CRY33 at both seedling and adult plant stages.33 cultivars (lines) had adult plant resistance,only showing resistance to stripe rust at adult stage.Based on the molecular screening,none of the 59 PNW cultivars (lines) had the polymorphic bands of linked markers to Yr10.There were 12,33 and 29 cultivars (lines) which had polymorphic bands of linked markers to Yr15,Yr18 and Yr39,accounting for 20,55 and 49% of the 59 PNW cultivars (lines),respectively.All these results suggested that Yr15,Yr18 and Yr39 were widespread among PNW cultivars (cultivars) and could be utilized in Chinese wheat stripe rust resistance breeding.

  1. Sensitivity and growth of twelve Elatior begonia cultivars to ozone

    Energy Technology Data Exchange (ETDEWEB)

    Reinert, R.A.; Nelson, P.V.

    1979-12-01

    Twelve cultivars of Elatior begonia (Begonia X hiemalis Fotsch.) were exposed to O/sub 3/ at 25 and 50 pphM. The 'Schwabenland' group, 'Whisper 'O' Pink', and 'Improved Krefeld Orange' were the most sensitive, whereas 'Ballerina', 'Mikkell Limelight', and 'Turo' were the least sensitive. 'Rennaisance', 'Heirloom' 'Nixe', and 'Fantasy' were intermediate in sensitivity. The dry weight of foliage (stems plus leaves) of 9 cultivars exposed to O/sub 3/ was significantly less than that of control plants. Ozone at 25 and 50 pphM inhibited flower growth (including peduncles) and development in 4 and 8 of the 12 cultivars, respectively. Differences in flower weight ranged from 43 to 105% of the control at 25 pphM and from 25 to 98% of the control at 50 pphM, depending on cultivar. 1 table.

  2. Population structure and genetic bottleneck in sweet cherry estimated with SSRs and the gametophytic self-incompatibility locus

    Directory of Open Access Journals (Sweden)

    Mariette Stéphanie

    2010-08-01

    Full Text Available Abstract Background Domestication and breeding involve the selection of particular phenotypes, limiting the genomic diversity of the population and creating a bottleneck. These effects can be precisely estimated when the location of domestication is established. Few analyses have focused on understanding the genetic consequences of domestication and breeding in fruit trees. In this study, we aimed to analyse genetic structure and changes in the diversity in sweet cherry Prunus avium L. Results Three subgroups were detected in sweet cherry, with one group of landraces genetically very close to the analysed wild cherry population. A limited number of SSR markers displayed deviations from the frequencies expected under neutrality. After the removal of these markers from the analysis, a very limited bottleneck was detected between wild cherries and sweet cherry landraces, with a much more pronounced bottleneck between sweet cherry landraces and modern sweet cherry varieties. The loss of diversity between wild cherries and sweet cherry landraces at the S-locus was more significant than that for microsatellites. Particularly high levels of differentiation were observed for some S-alleles. Conclusions Several domestication events may have happened in sweet cherry or/and intense gene flow from local wild cherry was probably maintained along the evolutionary history of the species. A marked bottleneck due to breeding was detected, with all markers, in the modern sweet cherry gene pool. The microsatellites did not detect the bottleneck due to domestication in the analysed sample. The vegetative propagation specific to some fruit trees may account for the differences in diversity observed at the S-locus. Our study provides insights into domestication events of cherry, however, requires confirmation on a larger sampling scheme for both sweet cherry landraces and wild cherry.

  3. A Phase Transition for Circle Maps and Cherry Flows

    Science.gov (United States)

    Palmisano, Liviana

    2013-07-01

    We study C 2 weakly order preserving circle maps with a flat interval. The main result of the paper is about a sharp transition from degenerate geometry to bounded geometry depending on the degree of the singularities at the boundary of the flat interval. We prove that the non-wandering set has zero Hausdorff dimension in the case of degenerate geometry and it has Hausdorff dimension strictly greater than zero in the case of bounded geometry. Our results about circle maps allow to establish a sharp phase transition in the dynamics of Cherry flows.

  4. A Phase Transition for Circle Maps and Cherry Flows

    CERN Document Server

    Palmisano, Liviana

    2012-01-01

    We study $C^{2}$ weakly order preserving circle maps with a flat interval. The main result of the paper is about a sharp transition from degenerate geometry to bounded geometry depending on the degree of the singularities at the boundary of the flat interval. We prove that the non-wandering set has zero Hausdorff dimension in the case of degenerate geometry and it has Hausdorff dimension strictly greater than zero in the case of bounded geometry. Our results about circle maps allow to establish a sharp phase transition in the dynamics of Cherry flows.

  5. Variation in Broccoli Cultivar Phytochemical Content under Organic and Conventional Management Systems: Implications in Breeding for Nutrition

    NARCIS (Netherlands)

    Renaud, E.N.C.; Lammerts Van Bueren, E.; Myers, J.R.; Caldas Paulo, M.J.; Eeuwijk, van F.A.; Zhu, N.; Juvik, J.A.

    2014-01-01

    Organic agriculture requires cultivars that can adapt to organic crop management systems without the use of synthetic pesticides as well as genotypes with improved nutritional value. The aim of this study encompassing 16 experiments was to compare 23 broccoli cultivars for the content of phytochemic

  6. 酸樱桃新品种‘奥德’%A New Sour Cherry Cultivar ‘Aode’

    Institute of Scientific and Technical Information of China (English)

    蔡宇良; 王玉; 邱蓉; 冯瑛; 张雪; 宛甜; 何恒流

    2014-01-01

    ‘奥德’是从野生酸樱桃中经实生选种选育出的新品种.单果质量5.5 g,总糖为10.36%,总酸度为1.45%,可溶性蛋白质1.40%,果实出汁率达到85.2%.矮化,抗旱、耐盐碱、抗裂果、抗病虫性强,固地性强,已在陕西、甘肃、山西、河南、山东等地栽培.

  7. Use of multiline cultivars and cultivar mixtures for disease management.

    Science.gov (United States)

    Mundt, C C

    2002-01-01

    The usefulness of mixtures (multiline cultivars and cultivar mixtures) for disease management has been well demonstrated for rusts and powdery mildews of small grain crops. Such mixtures are more useful under some epidemiological conditions than under others, and experimental methodology, especially problems of scale, may be crucial in evaluating the potential efficacy of mixtures on disease. There are now examples of mixtures providing both low and high degrees of disease control for a wide range of pathosystems, including crops with large plants, and pathogens that demonstrate low host specificity, or are splash dispersed, soilborne, or insect vectored. Though most analyses of pathogen evolution in mixtures consider static costs of virulence to be the main mechanism countering selection for pathogen complexity, many other potential mechanisms need to be investigated. Agronomic and marketing considerations must be carefully evaluated when implementing mixture approaches to crop management. Practical difficulties associated with mixtures have often been overestimated, however, and mixtures will likely play an increasingly important role as we develop more sustainable agricultural systems.

  8. �Saruman� Apple Cultivar

    Directory of Open Access Journals (Sweden)

    Radu SESTRAS

    2010-06-01

    Full Text Available The apple cultivar Saruman was obtained at Fruit Research Station Cluj, Romania (FRS Cluj, and homologated in 2007. Saruman was released by hybridization between Cluj III-VI-5-26 selection (Parmain dOr, open pollinated and NJ 46. The trees are vigorous, spreading shape, and with medium crop yield. The fruits have large size, conic shape and mostly red (purple coloration; they have white flesh with a sweet, crisp, aromatic flavour and low acidity. Fruits become ripe in the last decade of August, first decade of September and the fruits are proper for dessert and well suited for cooking, applesauce, cider, pies.

  9. Development of deficit irrigation scheduling strategies for 'Prime Giant' sweet cherry

    Science.gov (United States)

    Blanco, Víctor; Domingo, Rafael; Torres, Roque; Pérez Pastor, Alejandro; García, Manuel; López, Juan Antonio

    2016-04-01

    Precision regulated deficit irrigation scheduling is useful for improving water productivity and ensuring crop production sustainability. This form of water management requires continuous monitoring in order to know soil and/or plant water status at all times. Water status sensors are key tools for modulating irrigation water amounts. The objective of this work was to study the physiological and agronomic response of cherry trees to different irrigation treatments based on crop evapotranspiration (ETc). However, the final purpose was to establish threshold values of water stress indicators, which can be considered of practical applicability in automatic irrigation scheduling. The experiment was carried out in 2015 in a 0.5 ha commercial plot of 'Prime Giant' cherry [Prunus avium (L.)] in SE Spain. Three treatments were studied i) T110, irrigated above the maximum crop water requirements (110% of ETc), ii) T85, sustained deficit irrigation, irrigated to satisfy 85% of ETc, throughout the growing season, and iii) T100-55, regulated deficit irrigation with different water deficit levels: 100% and 55% of ETc during pre- and postharvest, respectively. Each treatment was randomly distributed in blocks and run in triplicate. Soil and plant water status were assessed from the soil matric potential and volumetric water content (Ym and Ov), midday stem and fruit water potential (Ys and Yf), maximum daily trunk shrinkage (MDS), daily trunk growth rate (TGR), stomatal conductance (gs), photosynthesis (Pn) and transpiration rates (E). Vegetative growth, yield and the quality of the fruit were also evaluated. Ys and MDS signal intensity were used as the main indicators of water stress. The water applied during the 2015 growing season was 7190, 5425 and 4225 m3 ha-1 for T110, T85 and T100-55, respectively. The mean values of Ys during pre- and postharvest were -0.51, -0.57, -0.54 and -0.65, -0.77 and -0.97 MPa in T110, T85 and T100-55, respectively, while Yf was -1.20, -1.36, -1

  10. Molecular Identification and Cultivar Fingerprints of Prunus persica (L.)Batsch Germplasms

    Institute of Scientific and Technical Information of China (English)

    SUN Shu-xia; LI Jing; JIANG Guo-liang; CHEN Dong; XIE Hong-jiang; TU Mei-yan

    2010-01-01

    [Objective]The aim was to study the molecular identification and cultivar fingerprints of Prunus persica(L.)Batsch germplasms.[Method]Sixty peach genotypes,representing China common local cultivars and European samples were screened by microsatellites(simple sequence repeats,SSRs)and Inter-Simple Sequence Repeat(ISSR)markers.[Result]26 reproducible bands were amplified by Nine SSR primers,and 24 of which were polymorphic; 236 bands were amplified by 30 ISSR primers,and 113 of which were polymorphic.31 genotypes were discriminated with 1-3 distinct polymorphic bands generated from the primers ISSR and SSR.Seven cultivar-specific ISSR fragments and two SSR unique alleles obtained from this study were available to be converted into Sequence Characterized Amplified Region(SCAR)markers.The genetic similarity coefficient(GS)estimated from these molecular data averaged were 0.939(ranged from 0.856 to 0.983)for ISSR and0.646(ranged from 0.240 to 1.000)for SSR,respectively.The combined grouping association indicated that most local Chinese peach cultivars and exotic accessions were clustered together.This could be related to the mode of introduction and maintenance of the peach cultivars involving limited foundation germplasm,exchange of cultivars between plantations,and periodic development of new recombinant cultivars following sexual reproduction.[Conclusion]The results obtained in this work would help to improve the conservation,molecular identification and management of peach germplasm in breeding.

  11. Cell wall alterations in the leaves of fusariosis-resistant and susceptible pineapple cultivars.

    Science.gov (United States)

    de Farias Viégas Aquije, Glória Maria; Zorzal, Poliana Belisário; Buss, David Shaun; Ventura, José Aires; Fernandes, Patricia Machado Bueno; Fernandes, Antonio Alberto Ribeiro

    2010-10-01

    Fusariosis, caused by the fungus Fusarium subglutinans f. sp. ananas (Syn. F. guttiforme), is one of the main phytosanitary threats to pineapple (Ananas comosus var. comosus). Identification of plant cell responses to pathogens is important in understanding the plant-pathogen relationship and establishing strategies to improve and select resistant cultivars. Studies of the structural properties and phenolic content of cell walls in resistant (Vitoria) and susceptible (Perola) pineapple cultivars, related to resistance to the fungus, were performed. The non-chlorophyll base of physiologically mature leaves was inoculated with a conidia suspension. Analyses were performed post-inoculation by light, atomic force, scanning and transmission electron microscopy, and measurement of cell wall-bound phenolic compounds. Non-inoculated leaves were used as controls to define the constitutive tissue characteristics. Analyses indicated that morphological differences, such as cell wall thickness, cicatrization process and lignification, were related to resistance to the pathogen. Atomic force microscopy indicated a considerable difference in the mechanical properties of the resistant and susceptible cultivars, with more structural integrity, associated with higher levels of cell wall-bound phenolics, found in the resistant cultivar. p-Coumaric and ferulic acids were shown to be the major phenolics bound to the cell walls and were found in higher amounts in the resistant cultivar. Leaves of the resistant cultivar had reduced fungal penetration and a faster and more effective cicatrization response compared to the susceptible cultivar.

  12. Diversity of rhizosphere soil arbuscular mycorrhizal fungi in various soybean cultivars under different continuous cropping regimes.

    Directory of Open Access Journals (Sweden)

    Weiguang Jie

    Full Text Available Recent studies have shown that continuous cropping in soybean causes substantial changes to the microbial community in rhizosphere soil. In this study, we investigated the effects of continuous cropping for various time periods on the diversity of rhizosphere soil arbuscular mycorrhizal (AM fungi in various soybean cultivars at the branching stage. The soybean cultivars Heinong 37 (an intermediate cultivar, Heinong 44 (a high-fat cultivar and Heinong 48 (a high-protein cultivar were seeded in a field and continuously cropped for two or three years. We analyzed the diversity of rhizosphere soil AM fungi of these soybean plants at the branching stage using morphological and denaturing gradient gel electrophoresis (DGGE techniques. The clustering analysis of unweighted pair-group method with arithmetic averages (UPGMA was then used to investigate the AM fungal community shifts. The results showed that increasing the number of years of continuous cropping can improve the colonization rate of AM fungi in different soybean cultivars at the branching stage. The dominant AM fungi in the experimental fields were Funneliformismosseae and Glomus spp. The number of years of continuous cropping and the soybean cultivar both had obvious effects on the diversity of AM fungi, which was consistent with the results of colonization rate analysis. This study establishes a basis for screening dominant AM fungi of soybean. In addition, the results of this study may be useful for the development of AM fungal inoculants.

  13. CULTIVAR IMPACT ON THE CHEMICAL CONTENT AND GRAIN TECHNOLOGICAL QUALITIES OF SOME DURUM WHEAT CULTIVARS

    Directory of Open Access Journals (Sweden)

    Tanko Kolev

    2011-11-01

    Full Text Available A comparative experiment was carried out with five Bulgarian and five foreign durum wheat cultivars. The aim of the experiment was to determine the chemical content and grain technological quality of some Bulgarian and foreign durum wheat cultivars grown under the agriecological conditions of Southern Bulgaria. The Vazhod cultivar proved to give the highest durum wheat grain yield, followed by Beloslava cultivar. Out of the foreign durum wheat cultivars the Durumko was notable for its higher productivity. The crude protein content in the grain was highest in Zagorka, Yavor and Yukon. The highest yield of gluten was reported in Beloslava, Vazhod and Zagorka.

  14. Ascorbic acid metabolism during sweet cherry (Prunus avium) fruit development.

    Science.gov (United States)

    Liang, Dong; Zhu, Tingting; Ni, Zhiyou; Lin, Lijin; Tang, Yi; Wang, Zhihui; Wang, Xun; Wang, Jin; Lv, Xiulan; Xia, Hui

    2017-01-01

    To elucidate metabolism of ascorbic acid (AsA) in sweet cherry fruit (Prunus avium 'Hongdeng'), we quantified AsA concentration, cloned sequences involved in AsA metabolism and investigated their mRNA expression levels, and determined the activity levels of selected enzymes during fruit development and maturation. We found that AsA concentration was highest at the petal-fall period (0 days after anthesis) and decreased progressively during ripening, but with a slight increase at maturity. AsA did nevertheless continue to accumulate over time because of the increase in fruit fresh weight. Full-length cDNAs of 10 genes involved in the L-galactose pathway of AsA biosynthesis and 10 involved in recycling were obtained. Gene expression patterns of GDP-L-galactose phosphorylase (GGP2), L-galactono-1, 4-lactone dehydrogenase (GalLDH), ascorbate peroxidase (APX3), ascorbate oxidase (AO2), glutathione reductase (GR1), and dehydroascorbate reductase (DHAR1) were in accordance with the AsA concentration pattern during fruit development, indicating that genes involved in ascorbic acid biosynthesis, degradation, and recycling worked in concert to regulate ascorbic acid accumulation in sweet cherry fruit.

  15. Cherry juice targets antioxidant potential and pain relief.

    Science.gov (United States)

    Kuehl, Kerry S

    2012-01-01

    Strenuous physical activity increases the risk of musculoskeletal injury and can induce muscle damage resulting in acute inflammation and decreased performance. The human body's natural response to injury results in inflammation-induced pain, swelling, and erythema. Among sports medicine physicians and athletic trainers, the mainstays of urgent treatment of soft tissue injury are rest, ice, compression, and elevation (RICE). In order to reduce pain and inflammation, anti-inflammatory agents such as non-steroidal anti-inflammatory drugs (NSAIDs) act on the multiple inflammatory pathways, which, although often very effective, can have undesirable side effects such as gastric ulceration and, infrequently, myocardial infarction and stroke. For centuries, natural anti-inflammatory compounds have been used to mediate the inflammatory process and often with fewer side effects. Tart cherries appear to possess similar effectiveness in treating the inflammatory reaction seen in both acute and chronic pain syndromes encountered among athletes and non-athletes with chronic inflammatory disease. This article reviews the antioxidant and anti-inflammatory effects of tart cherries on prevention, treatment, and recovery of soft tissue injury and pain.

  16. Soil covering in organic cultivation of onion cultivars

    OpenAIRE

    Leonardo Barreto Tavella; Regina Lúcia Felix Ferreira; Sebastião Elviro de Araújo Neto; Antônio Jussie da Silva Solino; Eliana Mara Napoli Corrêa de Paula da Silva; Maísa Pinto Bravin

    2015-01-01

    The soil preparation in horticulture, including organic, is characterized by intense soil tillage, which increases energy costs and unbalanced the environment. The organic system in onion cultivation has shown satisfactory results, however the soil covering use tends to improve the cultivation environment and may result in higher yields. The objective of this work was to evaluate the performance of onion cultivars in organic cultivation under different soil coverages. The experiment was co...

  17. Cannabis - from cultivar to chemovar.

    Science.gov (United States)

    Hazekamp, A; Fischedick, J T

    2012-01-01

    The medicinal use of Cannabis is increasing as countries worldwide are setting up official programs to provide patients with access to safe sources of medicinal-grade Cannabis. An important question that remains to be answered is which of the many varieties of Cannabis should be made available for medicinal use. Drug varieties of Cannabis are commonly distinguished through the use of popular names, with a major distinction being made between Indica and Sativa types. Although more than 700 different cultivars have already been described, it is unclear whether such classification reflects any relevant differences in chemical composition. Some attempts have been made to classify Cannabis varieties based on chemical composition, but they have mainly been useful for forensic applications, distinguishing drug varieties, with high THC content, from the non-drug hemp varieties. The biologically active terpenoids have not been included in these approaches. For a clearer understanding of the medicinal properties of the Cannabis plant, a better classification system, based on a range of potentially active constituents, is needed. The cannabinoids and terpenoids, present in high concentrations in Cannabis flowers, are the main candidates. In this study, we compared cultivars obtained from multiple sources. Based on the analysis of 28 major compounds present in these samples, followed by principal component analysis (PCA) of the quantitative data, we were able to identify the Cannabis constituents that defined the samples into distinct chemovar groups. The study indicates the usefulness of a PCA approach for chemotaxonomic classification of Cannabis varieties.

  18. Efficacy of tart cherry juice in reducing muscle pain during running: a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Elliot Diane L

    2010-05-01

    Full Text Available Abstract Background Long distance running causes acute muscle damage resulting in inflammation and decreased force production. Endurance athletes use NSAIDs during competition to prevent or reduce pain, which carries the risk of adverse effects. Tart cherries, rich in antioxidant and anti-inflammatory properties, may have a protective effect to reduce muscle damage and pain during strenuous exercise. This study aimed to assess the effects of tart cherry juice as compared to a placebo cherry drink on pain among runners in a long distance relay race. Methods The design was a randomized, double blind, placebo controlled trial. Fifty-four healthy runners (36 male, 18 female; 35.8 ± 9.6 yrs ran an average of 26.3 ± 2.5 km over a 24 hour period. Participants ingested 355 mL bottles of tart cherry juice or placebo cherry drink twice daily for 7 days prior to the event and on the day of the race. Participants assessed level of pain on a standard 100 mm Visual Analog Scale (VAS at baseline, before the race, and after the race. Results While both groups reported increased pain after the race, the cherry juice group reported a significantly smaller increase in pain (12 ± 18 mm compared to the placebo group (37 ± 20 mm (p Conclusions Ingesting tart cherry juice for 7 days prior to and during a strenuous running event can minimize post-run muscle pain.

  19. Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia

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    Gavrilović Veljko

    2013-01-01

    Full Text Available During past few years a symptoms of plum and cherry bud necrosis were observed in some regions with significant cherry production in Serbia. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+---+. Symptoms similar to those observed in natural infection were obtained after artificial inoculation of cherry leaf scares and dormant one year old cherry shoots. Investigated strains as well as reference strain of P. syringae pv. morsprunorum cause the superficial necrosis on artificially inoculated immature cherry fruits, but negative results were recorded in immature pear and lemon fruit tests as well as syringae leaves and bean pods. Gelatin and aesculin tests were negative and tyrosinase and tartrate were positive. Investigated strains isolated from necrotic cherry buds had identical REP-PCR pattern with reference strain of P. syringae pv. morsprunorum. On the basis of obtained results, it was concluded that this bacterium is causal agent of cherry trees bud necrosis in Serbia. [Projekat Ministarstva nauke Republike Srbije, br. 31018 i br. 173026

  20. Effects of Soybean Cultivars on Soymilk Quality

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    Aziadekey, M.

    2001-01-01

    Full Text Available Soymilk was prepared from twelve soybean cultivars grown under the same environmental conditions to evaluate their effects on soymilk characteristics. Significant correlations were observed between the Chemical composition of the seeds and the resultant soymilk. Soymilk solids were significantly affected by seed size and seed phosphorus contents. Cultivars with dark hilum produced soymilk with less attractive colour.

  1. Tracking cholesterol/sphingomyelin-rich membrane domains with the ostreolysin A-mCherry protein.

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    Matej Skočaj

    Full Text Available Ostreolysin A (OlyA is an ∼15-kDa protein that has been shown to bind selectively to membranes rich in cholesterol and sphingomyelin. In this study, we investigated whether OlyA fluorescently tagged at the C-terminal with mCherry (OlyA-mCherry labels cholesterol/sphingomyelin domains in artificial membrane systems and in membranes of Madin-Darby canine kidney (MDCK epithelial cells. OlyA-mCherry showed similar lipid binding characteristics to non-tagged OlyA. OlyA-mCherry also stained cholesterol/sphingomyelin domains in the plasma membranes of both fixed and living MDCK cells, and in the living cells, this staining was abolished by pretreatment with either methyl-β-cyclodextrin or sphingomyelinase. Double labelling of MDCK cells with OlyA-mCherry and the sphingomyelin-specific markers equinatoxin II-Alexa488 and GST-lysenin, the cholera toxin B subunit as a probe that binds to the ganglioside GM1, or the cholesterol-specific D4 domain of perfringolysin O fused with EGFP, showed different patterns of binding and distribution of OlyA-mCherry in comparison with these other proteins. Furthermore, we show that OlyA-mCherry is internalised in living MDCK cells, and within 90 min it reaches the juxtanuclear region via caveolin-1-positive structures. No binding to membranes could be seen when OlyA-mCherry was expressed in MDCK cells. Altogether, these data clearly indicate that OlyA-mCherry is a promising tool for labelling a distinct pool of cholesterol/sphingomyelin membrane domains in living and fixed cells, and for following these domains when they are apparently internalised by the cell.

  2. Cherry picking in ESRD: an ethical challenge in the era of pay for performance.

    Science.gov (United States)

    Parker, J Clint

    2011-01-01

    In poorly designed pay-for-performance schemes in which case mix adjustments are not adequate, self-interest could lead nephrologists toward cherry picking dialysis patients. Cherry picking, however, is morally problematic. First, it may manifest itself as a subtle form of covert rationing which threatens to undermine patient trust. Second, it involves shifting the burden of caring for sicker (and less financially attractive) patients to other nephrologists and dialysis units that do not practice cherry picking, creating injustices in the health care system. Finally, it treats patients as mere means through which nephrologists achieve reimbursement instead of as persons possessing dignity and deserving of respect.

  3. Detecting local establishment strategies of wild cherry (Prunus avium L.

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    Gregorius Hans-Rolf

    2006-10-01

    Full Text Available Abstract Backround P. avium, a pioneer tree species that colonizes early forest successional stages, is assumed to require an effective strategy allowing stably repeatable rounds of local establishment, dispersal and local extinction. Consequently, the early replacement of cherry by climax tree species makes the establishment of several local generations very unlikely, especially in central European continuous cover forests. This has to be seen in connection with the mixed reproduction system involving asexual reproduction as a complementary adaptational strategy. Tests of the local establishment of wild cherry must therefore consider the possibility of first generation establishment via seedling recruitment potentially followed by an asexual generation (root suckering. Successful establishment can therefore be determined only among adult individuals with the option of detecting vegetative reproduction at these stages. To test the implied suggestion about local establishment strategies of wild cherry, nuclear microsatellites were used to analyse patterns of asexual propagation among adult stages that have been subjected to one of two major types of forest management. These management types, the historical "coppice with standards system" (CWS and the "high forest system" (HFS, can be reasonably assumed to have affected the reproduction system of P. avium. Results Clear differences were found in the reproduction pattern between two stands representing the two forest management types: 1 Clonal propagation is observed in both management systems, but with a distinctly higher frequency in the CWS. Hence, sexual recruitment as a first local generation is followed by a second asexual generation in both, whereas in the CWS there is evidence for an additional clonal generation. 2 The estimation of amounts of clonal reproduction critically depends on the assumptions about multilocus gene associations. This is revealed by the application of newly developed

  4. Differentiation of triticale cultivars through FISH karyotyping of their rye chromosomes.

    Science.gov (United States)

    Fradkin, Maia; Ferrari, María Rosa; Espert, Shirley Mary; Ferreira, Víctor; Grassi, Ezequiel; Greizerstein, Eduardo José; Poggio, Lidia

    2013-05-01

    The aim of this work was to cytogenetically characterize triticale cultivars through fluorescence in situ hybridization (FISH) analysis of their rye chromosomes. In the present work, we studied six cultivars of triticale ('Cayú-UNRC', 'Cumé-UNRC', 'Genú-UNRC', 'Ñinca-UNRC', 'Quiñé-UNRC', and 'Tizné-UNRC'), released by the Universidad Nacional de Río Cuarto (UNRC), Córdoba, Argentina. The cultivars were obtained from the International Center for the Improvement of Maize and Wheat (CIMMYT) and improved for fresh forage, haymaking, and feed grain at UNRC. The distribution and organization of highly repetitive DNA sequences of Secale cereale (pSc74, pSc200, pSc250, and pSc119.2) using FISH analyses revealed a specific localization of the signals for several rye chromosomes, which allowed us to distinguish the cultivars. Cluster analysis showed a great cytogenetic similarity among the rye cultivars used to originate these hybrids. The knowledge of the variability among triticale cultivars is necessary to propose future crosses in breeding programs. This study will also be valuable to identify commercial seeds and to analyze the possible association between agronomic characters and the presence of certain rye chromosomes or specific regions in these chromosomes.

  5. �Saruman� Apple Cultivar

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    Radu SESTRAS

    2010-06-01

    Full Text Available The apple cultivar �Saruman� was obtained at Fruit Research Station Cluj, Romania (FRS Cluj, and homologated in 2007. �Saruman� was released by hybridization between �Cluj III-VI-5-26� selection (�Parmain d�Or�, open pollinated and �NJ 46�. The trees are vigorous, spreading shape, and with medium crop yield. The fruits have large size, conic shape and mostly red (purple coloration; they have white flesh with a sweet, crisp, aromatic flavour and low acidity. Fruits become ripe in the last decade of August, first decade of September and the fruits are proper for dessert and well suited for cooking, applesauce, cider, pies.

  6. �Sauron� Apple Cultivar

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    Radu SESTRAS

    2010-06-01

    Full Text Available The new apple cultivar Sauron was obtained at Fruit Research Station Cluj, Romania (FRS Cluj, and homologated in 2007. Sauron was identified in open pollinated population of Cluj 3/83 selection [Cluj III-VI-5-26 selection (Parmain dOr, open pollinated x NJ 46]. The trees have moderate vigour, upright then spread, with moderate productivity. Fruits are medium to large, usually red, with a portion being greenish or yellow-green and purple red vertically striped. The fruit has good quality, being soft eating apple due to their lack of crispness. Quality indices include firmness, crispness and excellent flavour. Fruits become ripe between 15 August and 5 September and the fruits are good quality, being proper for dessert and industrialization (juice, applesauce, pies, and cider.

  7. Cultivar de soja BRS 66

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    Bertagnolli Paulo Fernando

    2002-01-01

    Full Text Available BRS 66, oriunda do cruzamento BR 83-147 x FT-Abyara, é indicada para cultivo no Rio Grande do Sul. Na média de 21 ambientes no RS, no período de 1993/94 a 1995/96, essa cultivar apresentou rendimento médio de grãos de 2.883 kg/ha. Apresenta resistência ao cancro-da-haste, à podridão-parda-da-haste, à mancha-olho-de-rã, à raça 1 de Phytophthora sojae, à pústula-bacteriana e ao oídio. Tem flor branca, pubescência marrom, tegumento da semente amarelo-fosco, hilo marrom e tipo de crescimento determinado. É de ciclo médio e apresenta estatura da planta de média a alta, com boa resistência ao acamamento.

  8. Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR

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    Seung-Yeol Lee

    2014-12-01

    Full Text Available The incidence of Cherry necrotic rusty mottle virus (CNRMV and Cherry green ring mottle virus (CGRMV have recently been occurred in Korea, posing a problem for sweet cherry cultivation. Since infected trees have symptomless leaves or ring-like spots on the pericarp, it is difficult to identify a viral infection. In this study, the incidence of CNRMV and CGRMV in sweet cherry in Gyeongbuk province was surveyed using a newly developed duplex reverse transcriptase polymerase chain reaction (RT-PCR method that can detect both viruses in a single reaction. CNRMV and CGRMV co-infection rates were 29.6%, 53.6%, and 17.6%, respectively, in samples collected from three different sites (Daegu, Gyeongju and Gyeongsan in Gyeongbuk province during 2012 and 2013. This duplex RT-PCR method offers a simple, rapid, and effective way of identifying CNRMV and CGRMV simultaneously in sweet cherry trees, which can aid in the management of viral infections that could undermine yield.

  9. Seasonal amounts of nutrients in Western cherry fruit fly (Diptera: Tephritidae) and their relation to nutrient availability on cherry plant surfaces.

    Science.gov (United States)

    Yee, Wee L; Chapman, Peter S

    2008-10-01

    Relatively little is known about the nutritional ecology of fruit flies in the genus Rhagoletis. In this study, nutrient amounts in male and female western cherry fruit fly, Rhagoletis indifferens Curran, and availability of nitrogen and sugar on surfaces of leaves, fruit, and extrafloral nectaries (EFNs) of sweet cherry trees, were determined from late May to late June 2005 and of sugar from EFNs from mid-May to late June 2007 in Washington state. Protein amounts in male and female flies did not differ over the season. Nitrogen was present on leaves, fruit, and EFNs during the sampling period, but amounts on leaves and fruit were lower in late May than the rest of the season. Sugar amounts in flies did not differ over the season. Sugar was present on leaf, fruit, and EFN surfaces all season, but amounts on all three were lower in late May than later in the season. Fructose and glucose were the predominant sugars on all plant surfaces, but sucrose was also present in nectar from EFNs. In outdoor and field cage experiments in 2004 and 2006, more flies survived when cherry branches with leaves and fruit were present than absent. Results suggest that R. indifferens maintains stable protein and sugar levels throughout the season because sufficient amounts of nutrients are found in cherry trees during this time and that increases in nutrient availability caused by ripening and damaged cherries later in the season do not result in increased amounts of nutrients in flies.

  10. Dietary Administration of Bacillus subtilis Enhances Growth Performance, Immune Response and Disease Resistance in Cherry Valley Ducks

    Science.gov (United States)

    Guo, Mengjiao; Hao, Guangen; Wang, Baohua; Li, Ning; Li, Rong; Wei, Liangmeng; Chai, Tongjie

    2016-01-01

    Given the promising results of applying Bacillus subtilis (B.subtilis) as a probiotic in both humans and animals, the aim of this study was to systematically investigate the effects of B. subtilis on growth performance, immune response and disease resistance in Cherry Valley ducks. At 28 d post-hatch (dph), ducks fed a diet with B. subtilis weighed significantly more, had higher relative immune organ weights (e.g., bursa of Fabricius, thymus, and spleen), and exhibited greater villus heights, villus height to crypt depth ratios (duodenum and jejunum), and shallower crypt depths in the duodenum than controls fed a normal diet (p < 0.05). Moreover, the major pro-inflammatory factors and antiviral proteins, as measured in the thymus and the spleen, were higher at 28 dph in ducks fed probiotics than those of 14 dph. After 28 d of feeding, the ducks were challenged with Escherichia coli (E. coli) and novel duck reovirus (NDRV), and ducks fed B. subtilis achieved survival rates of 43.3 and 100%, respectively, which were significantly greater than the control group's 20 and 83.3%. Altogether, diets with B. subtilis can improve Cherry Valley ducks' growth performance, innate immune response, and resistance against E. coli and NDRV. PMID:28008328

  11. Cherry Valley National Wildlife Refuge Final Feasibility Study and Environmental Assessment

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — In accordance with the National Environmental Policy Act (NEPA), as amended, the Service has developed a Final EA in response to the Cherry Valley National Wildlife...

  12. Extraction and characterization of montmorency (Prunus cerasus L.) sour cherry pit oil

    Science.gov (United States)

    Montmorency sour cherry (Prunus cerasus L.) pit oil was extracted and characterized by various methods including: gas chromatography (GC), liquid chromatography coupled with mass spectrometry (LC-MS), nuclear magnetic resonance (NMR), thermogravimetric analysis (TGA), differential scanning calorime...

  13. Genetic diversity in wild sweet cherries (Prunus avium) in Turkey revealed by SSR markers.

    Science.gov (United States)

    Ercisli, S; Agar, G; Yildirim, N; Duralija, B; Vokurka, A; Karlidag, H

    2011-06-21

    Wild sweet cherry (Prunus avium) trees are abundant in the northern part of Turkey, including the Coruh Valley. We analyzed 18 wild sweet cherry genotypes collected from diverse environments in the upper Coruh Valley in Turkey to determine genetic variation, using 10 SSR primers. These SSR primers generated 46 alleles; the number of alleles per primer ranged from 3 to 7, with a mean of 4.6. The primer PS12A02 gave the highest number of polymorphic bands (N = 7), while CPSCT010, UDAp-401 and UDAp-404 gave the lowest number (N = 3). Seven groups were separated in the dendrogram, although most of the genotypes did not cluster according to phenological and morphological traits. This level of genetic diversity in these wild sweet cherry genotypes is very high and therefore these trees would be useful as breeders for crosses between cultivated sweet cherry and wild genotypes.

  14. Increased root and shoot production during micropropagation of cherry and apple rootstalks: effect of subculture frequency.

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    Grant; Hammatt

    1999-11-01

    Shoot cultures of apple rootstock cv. M9 and cherry rootstock cv. F12/1 were established and then divided into several sub-lines that were subcultured at 28-42-day intervals. Consequently, similarly aged cultures received various numbers of subcultures. Cultures kept at 24 degrees C showed an increase in shoot and root production over time. There were differences in shoot and root production between apple lines, but there were no differences among sub-lines. In cherry, altering the subculture interval affected rooting competence, which increased with time. Cherry cultures maintained at 4 degrees C gave rise to cultures that were as easy to root as cultures kept at 24 degrees C with more frequent subculturing. We conclude that total time in culture is the most important factor bringing about physiological changes in these genotypes of micropropagated apple and cherry.

  15. Pseudohyperglycaemia in a comatose patient after picking cherries.

    Science.gov (United States)

    Derkenne, Clément; Lamblin, Antoine; Jost, Daniel; Tourtier, Jean-Pierre

    2016-10-28

    We report a case of pseudohyperglycaemia on a capillary blood glucose measurement taken from fingers stained with sugar (fructose). A 76-year-old patient with type 1 diabetes received emergency attention at home because of a coma. The first capillary blood glucose measurement collected from a finger revealed a concentration higher than the reference limits, misleading the clinician. After starting symptomatic treatment, a second blood glucose measurement was taken. This measurement, taken at the earlobe, revealed profound hypoglycaemia (0.89 mmol/L), which prompted the administration of appropriate treatment. The elevated initial capillary blood glucose measurement was linked to the presence of fructose on the fingers of the patient from picking cherries just before the patient fainted. After intravenous administration of glucose, the patient regained normal consciousness and had no sequelae despite the severity of the hypoglycaemia and delayed diagnosis. Pseudohyperglycaemia is rare, and delayed diagnosis frequently results in severe sequelae or death.

  16. High throughput sequencing reveals a novel fabavirus infecting sweet cherry.

    Science.gov (United States)

    Villamor, D E V; Pillai, S S; Eastwell, K C

    2017-03-01

    The genus Fabavirus currently consists of five species represented by viruses that infect a wide range of hosts but none reported from temperate climate fruit trees. A virus with genomic features resembling fabaviruses (tentatively named Prunus virus F, PrVF) was revealed by high throughput sequencing of extracts from a sweet cherry tree (Prunus avium). PrVF was subsequently shown to be graft transmissible and further identified in three other non-symptomatic Prunus spp. from different geographical locations. Two genetic variants of RNA1 and RNA2 coexisted in the same samples. RNA1 consisted of 6,165 and 6,163 nucleotides, and RNA2 consisted of 3,622 and 3,468 nucleotides.

  17. Rapid Propagation of Sweet and Sour Cherry Rootstocks

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    Dušica DORIĆ

    2014-12-01

    Full Text Available The paper presents a protocol for micropropagation of Prunus sp. rootstocks included in the sweet and sour cherry breeding program. Germplasm diversity for rootstock breeding derives from natural populations, where conditions and biological vectors for systematic infection with viral diseases are constantly present. The establishment of aseptic culture depends primarily on the explant type, as all selections were collected from natural habitat. For nearly all investigated selections, dormant buds were the favored source, due to enabling rosette initiation in more than 58% cases. In P. cerasus L. selections, 100% contamination was noted when shoot tips were used as an explant source. Significant influence of the double-phase medium on the number and height of multiplied shoots was observed in the standard cherry rootstock, ‘Gisela 6’. For P. fruticosa Pall., selection ‘SV1’ and ‘SV2’, and P. cerasus ‘D6’ selection, the double-phase medium also had a significant effect on the height of multiplied shoots, when compared to solid DKW (Driver and Kuniyuki Walnut medium. Genetic variability of selections within the investigated species resulted in variable plant rooting success. Adding Fe-EDDHA (Ethylenediamine di-2-hydroxy-phenyl acetate ferric in the 200 mg l-1 concentration to the rooting medium significantly enhanced the percentage of rooted plants. The highest rooting percentage was noted for ‘Gisela 6’ and ‘D6’ genotype at 1 mg l-1 IBA (indole-3-butyric acid, while 0.8 mg l-1 was the optimum concentration for P. mahaleb L. ‘M1’ selection. P. fruticosa genotypes required significantly higher IBA concentration for rooting (2.5 and 3.5 mg l-1.

  18. Tay-Sach disease with "cherry-red spot"--first reported case in Malaysia.

    Science.gov (United States)

    Chan, L Y; Balasubramaniam, S; Sunder, R; Jamalia, R; Karunakar, T V N; Alagaratnam, J

    2011-12-01

    We present a rare case of Tay-Sachs disease with retinal 'cherry-red spots' in a 19-month-old Malay child. Molecular genetic studies confirmed the diagnosis. The case highlights that 'cherry-red spot' is a useful clinical clue in Tay-Sachs disease and several other lysosomal storage disorders. It serves as an ideal illustration of the eye as a window to inborn error of metabolism.

  19. Solar heating and hot water system installed at Cherry Hill, New Jersey

    Science.gov (United States)

    1979-01-01

    The solar heating and hot water system installed in existing buildings at the Cherry Hill Inn in Cherry Hill, New Jersey is described in detail. The system is expected to furnish 31.5% of the overall heating load and 29.8% of the hot water load. The collectors are liquid evacuated tube type. The storage system is an above ground insulated steel water tank with a capacity of 7,500 gallons.

  20. Influence of freezing and storing cherry fruit on its nutritional value

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    Elena Vasylyshyna

    2016-06-01

    Full Text Available Background. Cherries are a valuable dietary raw material and possess medicinal properties. Considering the nutritional, medical and vitamin value of cherry fruits, the purpose of this research was to produce a scientific justification for preserving the quality of cherry fruits using different freezing methods. Material and methods. To do this, cherry fruits from the Lotovka (Cerasus vulgaris variety were frozen in various ways: packed in polyethylene bags (control; previously suspended in a 20% sugar solution and pack- ing frozen cherry in polyethylene bags; suspended in a 20% sugar solution with the addition of 4% ascorutin and frozen followed by pre-packaging in polyethylene bags; cherry fruits were frozen in a 20% sugar solution in plastic cups of 0.25 cm3; they were also frozen in a 20% sugar solution with the addition of 4% ascorutin in plastic cups. The frozen products were stored at a temperature not higher than –18°C for up to 6 months. Result. Studies have shown the appropriateness of freezing cherry fruits, particularly in a 20% sugar solution with the addition of 4% ascorutin. The advantages of these fruits are in ascorbic acid preservation in 1.5 times and reduction of tanning and coloring substances only by 27%, soluble solids to 7%, sugars – 4%, acids – 12%, in tasting evaluation of 5 points. Conclusions. Frozen cherry fruits in a 20% sugar solution with the addition of 4% ascorutin can be used in dietary nutrition for patients with cardiovascular diseases.

  1. Ripening-Dependent Changes in Antioxidants, Color Attributes, and Antioxidant Activity of Seven Tomato (Solanum lycopersicum L.) Cultivars

    Science.gov (United States)

    2016-01-01

    To evaluate the ripening-dependent changes in phytonutrients, seven commercial cultivars (two general and five cherry) of tomatoes were cultivated under greenhouse conditions. Fruits were harvested at breaker, turning, pink, light red, and red stages of each cultivar, and antioxidant contents, color attributes, and antioxidant activities were measured. During ripening process, lycopene content increased from the breaker to red stage, while lutein displayed the reverse accumulation pattern, with higher values during the breaker stage. In contrast, β-carotene showed the highest levels of synthesis in pink and light red stages. Furthermore, flavonoids (quercetin, rutin, naringenin, and luteolin) also showed similar ripening-dependent changes, with higher quantities in pink and light red stages. Ascorbic acid showed continuously increasing patterns throughout ripening until the red stage, while the accumulation of total phenolics was cultivar-dependent. These results indicate that each antioxidant compound has a unique pattern of accumulation and degradation during the ripening process. “Unicon” exhibited highest total carotenoid (110.27 mg/100 g), total phenol (297.88 mg GAE/100 g) and total flavonoid content (273.33 mg/100 g), and consequently highest antioxidant activity (2552.4 μmol TE/100 g) compared to other cultivars. Throughout the ripening processes, total phenolics showed the highest correlation with antioxidant activity, followed by β-carotene and total flavonoids. In conclusion, ripening in tomatoes is accompanied by incremental increases in various antioxidant compounds to some extent, as well as by concomitant increases in antioxidant activity. PMID:27668121

  2. Ripening-Dependent Changes in Antioxidants, Color Attributes, and Antioxidant Activity of Seven Tomato (Solanum lycopersicum L. Cultivars

    Directory of Open Access Journals (Sweden)

    Shiva Ram Bhandari

    2016-01-01

    Full Text Available To evaluate the ripening-dependent changes in phytonutrients, seven commercial cultivars (two general and five cherry of tomatoes were cultivated under greenhouse conditions. Fruits were harvested at breaker, turning, pink, light red, and red stages of each cultivar, and antioxidant contents, color attributes, and antioxidant activities were measured. During ripening process, lycopene content increased from the breaker to red stage, while lutein displayed the reverse accumulation pattern, with higher values during the breaker stage. In contrast, β-carotene showed the highest levels of synthesis in pink and light red stages. Furthermore, flavonoids (quercetin, rutin, naringenin, and luteolin also showed similar ripening-dependent changes, with higher quantities in pink and light red stages. Ascorbic acid showed continuously increasing patterns throughout ripening until the red stage, while the accumulation of total phenolics was cultivar-dependent. These results indicate that each antioxidant compound has a unique pattern of accumulation and degradation during the ripening process. “Unicon” exhibited highest total carotenoid (110.27 mg/100 g, total phenol (297.88 mg GAE/100 g and total flavonoid content (273.33 mg/100 g, and consequently highest antioxidant activity (2552.4 μmol TE/100 g compared to other cultivars. Throughout the ripening processes, total phenolics showed the highest correlation with antioxidant activity, followed by β-carotene and total flavonoids. In conclusion, ripening in tomatoes is accompanied by incremental increases in various antioxidant compounds to some extent, as well as by concomitant increases in antioxidant activity.

  3. Detection and quantification of the antioxidant melatonin in Montmorency and Balaton tart cherries (Prunus cerasus).

    Science.gov (United States)

    Burkhardt, S; Tan, D X; Manchester, L C; Hardeland, R; Reiter, R J

    2001-10-01

    The antioxidant melatonin was recently identified in a variety of edible plants and seeds in high concentrations. In plants, as in animals, melatonin is believed to function as a free radical scavenger and possibly in photoperiodism. In this study, melatonin was detected and quantified in fresh-frozen Balaton and Montmorency tart cherries (Prunus cerasus) using high-performance liquid chromatography. Both cherry species contain high levels of melatonin compared to the melatonin concentrations in the blood of mammals. Montmorency cherries (13.46 +/- 1.10 ng/g) contain approximately 6 times more melatonin than do Balaton cherries (2.06 +/- 0.17 ng/g). Neither the orchard of origin nor the time of harvest influenced the amount of melatonin in fresh cherries. The implication of the current findings is that consuming cherries could be an important source of dietary melatonin inasmuch as melatonin is readily absorbed when taken orally. Also, previously published data and the results presented here show that melatonin is not only endogenously produced but also present in the diet.

  4. Synergistic inhibition of interleukin-6 production in adipose stem cells by tart cherry anthocyanins and atorvastatin.

    Science.gov (United States)

    Zhou, Zhou; Nair, Muraleedharan G; Claycombe, Kate J

    2012-07-15

    Studies have shown positive correlations between inflammatory cytokines such as interleukin-6 (IL-6) and the development of chronic diseases including cardiovascular disease by activating C-reactive protein (CRP). Both atorvastatin calcium (lipitor) as well as flavonoid rich fruit such as tart cherry demonstrate potent anti-inflammatory effects on IL-6 secretion. In this study, we investigated whether tart cherry extract or specific anthocyanins contained in the tart cherry show synergistic anti-inflammatory effects with lipitor. Results showed that LPS-induced adipose stem cell secretion of IL-6 reduced with the addition of tart cherry extract, a mixture of tart cherry anthocyanins, and pure tart cherry cyanidin-3-O-glucoside (C3G) in a dose-dependent manner. Furthermore, lipitor and C3G exhibited synergistic effects in reducing LPS-induced IL-6 secretion from adipose stem cells. In conclusion, these results support potential benefits of using dietary phytochemicals in conjunction with pharmacological therapies to decrease adipose inflammation, drug doses, and ultimately, drug-induced adverse effects.

  5. Nutraceutical Value of Black Cherry Prunus serotina Ehrh. Fruits: Antioxidant and Antihypertensive Properties

    Directory of Open Access Journals (Sweden)

    Francisco J. Luna-Vázquez

    2013-11-01

    Full Text Available In Mexico black cherry (Prunus serotina Ehrh. fruits are consumed fresh, dried or prepared in jam. Considering the evidence that has linked intake of fruits and vegetables rich in polyphenols to cardiovascular risk reduction, the aim of this study was to characterize the phenolic profile of black cherry fruits and to determine their antioxidant, vasorelaxant and antihypertensive effects. The proximate composition and mineral contents of these fruits were also assessed. Black cherry fruits possess a high content of phenolic compounds and display a significant antioxidant capacity. High-performance liquid chromatography/mass spectrometric analysis indicated that hyperoside, anthocyanins and chlorogenic acid were the main phenolic compounds found in these fruits. The black cherry aqueous extract elicited a concentration-dependent relaxation of aortic rings and induced a significant reduction on systolic blood pressure in L-NAME induced hypertensive rats after four weeks of treatment. Proximate analysis showed that black cherry fruits have high sugar, protein, and potassium contents. The results derived from this study indicate that black cherry fruits contain phenolic compounds which elicit significant antioxidant and antihypertensive effects. These findings suggest that these fruits might be considered as functional foods useful for the prevention and treatment of cardiovascular diseases.

  6. Integrated Management of European Cherry Fruit Fly Rhagoletis cerasi (L.: Situation in Switzerland and Europe

    Directory of Open Access Journals (Sweden)

    Jürg Grunder

    2012-10-01

    Full Text Available The European cherry fruit fly, Rhagoletis cerasi (L. (Diptera: Tephritidae, is a highly destructive pest. The low tolerance for damaged fruit requires preventive insecticide treatments for a marketable crop. The phase-out of old insecticides threatens cherry production throughout the European Union (EU. Consequently, new management techniques and tools are needed. With the increasing number of dwarf tree orchards covered against rain to avoid fruit splitting, crop netting has become a viable, cost-effective method of cherry fruit fly control. Recently, a biocontrol method using the entomopathogenic fungus Beauveria bassiana has been developed for organic agriculture. However, for most situations, there is still a lack of efficient and environmentally sound insecticides to control this pest. This review summarizes the literature from over one hundred years of research on R. cerasi with focus on the biology and history of cherry fruit fly control as well as on antagonists and potential biocontrol organisms. We will present the situation of cherry fruit fly regulation in different European countries, give recommendations for cherry fruit fly control, show gaps in knowledge and identify future research opportunities.

  7. Integrated Management of European Cherry Fruit Fly Rhagoletis cerasi (L.): Situation in Switzerland and Europe.

    Science.gov (United States)

    Daniel, Claudia; Grunder, Jürg

    2012-10-16

    The European cherry fruit fly, Rhagoletis cerasi (L.) (Diptera: Tephritidae), is a highly destructive pest. The low tolerance for damaged fruit requires preventive insecticide treatments for a marketable crop. The phase-out of old insecticides threatens cherry production throughout the European Union (EU). Consequently, new management techniques and tools are needed. With the increasing number of dwarf tree orchards covered against rain to avoid fruit splitting, crop netting has become a viable, cost-effective method of cherry fruit fly control. Recently, a biocontrol method using the entomopathogenic fungus Beauveria bassiana has been developed for organic agriculture. However, for most situations, there is still a lack of efficient and environmentally sound insecticides to control this pest. This review summarizes the literature from over one hundred years of research on R. cerasi with focus on the biology and history of cherry fruit fly control as well as on antagonists and potential biocontrol organisms. We will present the situation of cherry fruit fly regulation in different European countries, give recommendations for cherry fruit fly control, show gaps in knowledge and identify future research opportunities.

  8. Carotenoid profiling in tubers of different potato (Solanum sp) cultivars: accumulation of carotenoids mediated by xanthophyll esterification.

    Science.gov (United States)

    Fernandez-Orozco, Rebeca; Gallardo-Guerrero, Lourdes; Hornero-Méndez, Dámaso

    2013-12-01

    The carotenoid profile of sixty potato cultivars (commercial, bred, old and native cultivars) has been characterised in order to provide information to be used in selective breeding programs directed to improve the nutritional value of this important staple food. Cultivars were segregated into three groups according to the major pigment in the carotenoid profile: violaxanthin (37 cultivars; especially those with higher carotenoid content), lutein (16 cultivars), and neoxanthin (7 cultivars). Other minor carotenoids were antheraxanthin, β-cryptoxanthin and β-carotene, while zeaxanthin was absent in all sample. The total carotenoid content ranged from 50.0 to 1552.0 μg/100 g dry wt, with an average value of about 435.3 μg/100 g dry wt. Sipancachi, Poluya and Chaucha native cultivars showed the highest carotenoid content (1020.0, 1478.2 and 1551.2 μg/100 g dry wt, respectively). Xanthophyll esters were present in most cultivars, mainly as diesterified forms, being observed a direct correlation between the carotenoid content and the esterified fraction, suggesting that the esterification process facilitates the accumulation of these lipophilic compounds within the plastids. Therefore, the presence of xanthophyll esters should be a phenotypic character to be included in the breeding studies, and more efforts should be dedicated to the understanding of the biochemical process leading to this structural modification of carotenoids in plants.

  9. Bioactivities and Iridoid Determination of a Beverage Containing Noni, Cornelian Cherries and Olive Leaf Extract

    Directory of Open Access Journals (Sweden)

    Chen X. Su

    2012-04-01

    Full Text Available The aim of the current study was to evaluate the iridoid content, as well as the in vitro and in vivo bioactivities, of a beverage containing noni fruit, Cornelian cherries, and olive leaf extract (Thrive Adaptogenics Max. The average total iridoid content of the beverage was 2.09 mg/mL. The major iridoids present were identified as asperulosidic acid, deacetylasperulosidic acid, oleuropein, morroniside, loganic acid, and loganin. In the 2, 2-Diphenylpicrylhydrazyl (DPPH radical scavenging assay, remarkably high in vitro antioxidant activity was observed, with an IC50 of 3.8 :L/mL. In vivo bioactivities were evaluated in type 2 diabetic Sprague Dawley rats. In a dose-dependent manner, Thrive Adaptogenics Max reduced abnormal weight gain, blood glucose levels, and serum Advanced Glycation End products (AGEs, as well as improved immunity via increased T cell counts and CD4+/CD8+ ratios. These results suggest that this blend of ingredients is beneficial for improving and maintaining health in the general population, as well as among those with metabolic imbalance.

  10. Flower colours and pigments in tulip cultivars

    NARCIS (Netherlands)

    Eijk, van J.P.; Keulen, van H.A.; Dijk, van A.J.

    1987-01-01

    Aanvullend op eerder onderzoek, waarbij bijna 500 oude en nieuwe tulpencultivars op bloempigmenten werden geanalyseerd, worden in tabelvorm van diezelfde cultivars nu de bloemkleur en de relatieve hoeveelheden carotenoiden, delfinidine, cyanidine, pelargonidine en flavonolen aangegeven

  11. Investigation the Response of Rapeseed Cultivars to Moisture Regimes in Different Growth Stages

    Directory of Open Access Journals (Sweden)

    Abouzar ABBASIAN

    2011-10-01

    Full Text Available In order to evaluate the response of rapeseed cultivars to moisture regimes and correlation analysis in different growth stages, a field experiment was conducted in 2006-7 in seed and plant improvement institute of Karaj, Iran. Results showed that number of pods per plant, pod length, stem diameter, 1000-seed weight, oil seed content and branch numbers per plant were reduced significantly under water deficit stress. Also, grain yield was decreased more than biological yield and this resulted in decreased harvest index. Correlation coefficient analysis revealed that number of pods per plant, number of seeds per main and secondary pod, 1000-seed weight, pod length, biological yield and oil seed content had positive and significant correlation with seed yield. Stopping the irrigation from flowering stage also had undesirable effect on seed yield and its components. Among cultivars, Opera cultivar produced more seed yield (4053 kg ha-1 than the others under normal irrigation and under water deficit stress conditions, Zarfam cultivar had the maximum seed yield during stem elongation (3569 kg ha-1, flowering (2135 kg ha-1 and podding stages (2476 kg ha-1. Okapi had the least changes of oil content than other cultivars. Based on the result of this study can be concluded that Zarfam cultivar had better capability to tolerate drought stress and could produce greater seed yield under stress conditions

  12. Stability of growth periods traits for soybean cultivars across multiple locations

    Institute of Scientific and Technical Information of China (English)

    WANG Xiao-bo; WANG Hui-cai; WANG Rui-zhen; ZHOU Rong; CHEN Huai-zhu; CHANG Ru-zhen; QIU Li-juan; LIU Zhang-xiong; YANG Chun-yan; XU ran; LU Wei-guo; ZHANG Li-feng; WANG Qian; WEI Su-hong; YANG Chun-ming

    2016-01-01

    The growth periods (GPs, from planting/emergence to reproductive stage 8 (R8) of soybean cultivars vary in different ecological regions, especialy in China with a very complex soybean cropping system. In this study, a 3-yr experimental study was undertaken in three geographical locations of China from 2008 to 2010, including the Northeast (40.66–45.85°N), Huang-Huai (34.75–38.04°N) and southern (22.82–30.60°N) eco-regions with about 250 accessions in each region to clarify the classiifcation of maturity group (MG) and identify the cultivars with stable GP to increase the knowledge about the GP distribution of soybean cultivars in China. GPs of soybean cultivars in different eco-regions were signiifcant different with a gradual decrease from 115–125 d in the Northeast part to the 85–100 d in the southern part of China. The geographical location was the major factor for GP of cultivars from the Northeast, while the year of planting was the major factor affecting the stability of GPs in Huang-Huai summer and southern summer soybean. AMMI2 (additive main effects and multiplicative interaction)-Biplot analysis showed that the GPs of soybean cultivars from the Northeast eco-region have a comparatively satisfactory environmental stability. Moreover, soybean cultivars with moderate GP/MG and stable environment adaptabil-ity in different eco-regions were identiifed based on the linear regression and AMMI analysis, which was important for the accurate classiifcation of soybean MGs in future. Taken together, our results relfected the genetic diversity, geographical distribution and environmental stability of the Chinese soybean GP trait. Soybean cultivars with stable GP for various Chinese eco-regions would be beneifcial for Chinese soybean genetic improvement, varietal introduction, exchange, and soybean breeding program for wide adaptability.

  13. Comparing salt tolerance of beet cultivars and their halophytic ancestor: consequences of domestication and breeding programmes.

    Science.gov (United States)

    Rozema, Jelte; Cornelisse, Danny; Zhang, Yuancheng; Li, Hongxiu; Bruning, Bas; Katschnig, Diana; Broekman, Rob; Ji, Bin; van Bodegom, Peter

    2014-12-09

    Salt tolerance of higher plants is determined by a complex set of traits, the timing and rate of evolution of which are largely unknown. We compared the salt tolerance of cultivars of sugar beet and their ancestor, sea beet, in hydroponic studies and evaluated whether traditional domestication and more recent breeding have changed salt tolerance of the cultivars relative to their ancestor. Our comparison of salt tolerance of crop cultivars is based on values of the relative growth rate (RGR) of the entire plant at various salinity levels. We found considerable salt tolerance of the sea beet and slightly, but significantly, reduced salt tolerance of the sugar beet cultivars. This indicates that traditional domestication by selection for morphological traits such as leaf size, beet shape and size, enhanced productivity, sugar content and palatability slightly affected salt tolerance of sugar beet cultivars. Salt tolerance among four sugar beet cultivars, three of which have been claimed to be salt tolerant, did not differ. We analysed the components of RGR to understand the mechanism of salt tolerance at the whole-plant level. The growth rate reduction at higher salinity was linked with reduced leaf area at the whole-plant level (leaf area ratio) and at the individual leaf level (specific leaf area). The leaf weight fraction was not affected by increased salinity. On the other hand, succulence and leaf thickness and the net assimilation per unit of leaf area (unit leaf rate) increased in response to salt treatment, thus partially counteracting reduced capture of light by lower leaf area. This compensatory mechanism may form part of the salt tolerance mechanism of sea beet and the four studied sugar beet cultivars. Together, our results indicate that domestication of the halophytic ancestor sea beet slightly reduced salt tolerance and that breeding for improved salt tolerance of sugar beet cultivars has not been effective.

  14. Quantification of energy from early cultivars of sugarcane; Quantificacao da energia a partir de cultivares precoces de cana-de-acucar

    Energy Technology Data Exchange (ETDEWEB)

    Santin, Ivan; Silva Neto, Helio F. da; Tasso Junior, Luiz Carlos; Marques, Diogo; Marques, Marcos O. [Universidade Estadual Paulista (FCAV/UNESP), Jabotical, SP (Brazil). Fac. de Ciencias Agrarias e Veterinarias. Dept. de Tecnologia], E-mail: santinivan@hotmail.com

    2010-07-01

    The success of energy generation from biomass of sugarcane is related to the amount of bagasse produced and the improvements of the equipment involved in the thermal balance of the sugarcane industrialization process. In this sense, the objective of this study was to quantify the production and productivity of bagasse in different early cultivars of sugarcane. The experimental design was a randomized block design with 6 treatments (cultivars) and 3 replications. The experiment was carried out at FCAV/UNESP-Jaboticabal. To calculate the productivity, the number of stems was counted in a meter, the weight of stems was obtained. The percentage of fiber for each cultivar was determined by calculating the estimated production and productivity of mulch. Using these values to estimate the potential number of people who would benefit from the energy generated by the combustion of bagasse an evolving process of cogeneration power. Cultivar CTC 16 (best results), showed the highest value of TCH (100.66 t ha{sup -1}), production of bagasse (315.66 kg t {sup -1}), any product residue (32.00 t ha{sup -1}) and people who could benefit (9.33 x 106). The Cultivars SP91-1049 and IACSP93-3046 presented the results less satisfactory. (author)

  15. Exploring the genetic variability in water use efficiency: Evaluation of inter and intra cultivar genetic diversity in grapevines.

    Science.gov (United States)

    Tortosa, Ignacio; Escalona, José Mariano; Bota, Josefina; Tomás, Magdalena; Hernández, Esther; Escudero, Enrique García; Medrano, Hipólito

    2016-10-01

    Genetic improvement of crop Water Use Efficiency (WUE) is a general goal because the increasing water scarcity and the trend to a more sustainable agriculture. For grapevines, this subject is relevant and need an urgent response because their wide distribution in semi-arid areas. New cultivars are difficult to introduce in viticulture due to the narrow dependency of consumer appreciation often linked to a certain particular wine taste. Clones of reputed cultivars would presumably be more accepted but little is known on the intra-cultivar genetic variability of the WUE. The present work compares, on the basis of two field assays, the variability of intrinsic water use efficiency (WUEi) in a large collection of cultivars in contrast with a collection of clones of Tempranillo cultivar. The results show that clonal variability of WUEi was around 80% of the inter-cultivar, thus providing a first assessment on the opportunity for clonal selection by WUE. Plotting the WUEi data against stem water potential or stomatal conductance it was possible to identify cultivars and clones out of the confidence intervals of this linear regression thus with significantly higher and lower WUEi values. The present results contribute to open the expectative for a genetic improvement of grapevine WUE.

  16. Design of Cherry Picking Robot—Based on PLC High-speed Parallel Automatic Control%樱桃采摘机器人设计—基于PLC高速并联自动化控制

    Institute of Scientific and Technical Information of China (English)

    张丽; 郭素娜; 汪小志

    2016-01-01

    为了降低樱桃采摘机器人采摘过程中的樱桃破碎率,提高机器人的工作效率,设计了一款新的基于模糊控制和高速并联自动化控制的机器人. 该机器可以利用PC上位机对樱桃图像进行采集,并可以对图像进行二值化、膨胀腐蚀处理,从而成功地识别成熟樱桃;同时,可以使用模糊PLC控制方法对采摘机器人的响应角度误差进行控制. 为了验证机器人的性能,使用樱桃采摘试验的方法对樱桃采摘机器人的性能进行了测试. 结果表明:高速并联自动化控制的樱桃采摘机器人总体采摘时间有了明显的缩短,工作效率有所提高;通过模糊控制可以使采摘机器人角度的响应平稳地达到指定角度,且没有出现大的超调量,有利于樱桃的采摘,降低了破碎率.%In order to decrease the broken rate of picking robot in the process of picking cherry , and enhances the work-ing efficiency of the robot , a new robot based on fuzzy control and high speed parallel automation control has been de-signed .The machine can use PC for cherry image acquisition , and can be conducted for image binarization , expansion corrosion process , thus successful identification of ripe cherry , you can use the fuzzy PLC control method , response to control the Angle error of picking robot .In order to verify the performance of the robot , the use of cherry picking experi-mental method for cherry picking robot performance was tested .Through test , found that high speed parallel automatic control overall pick cherry time was shortened obviously , improve the work efficiency of the robot , through fuzzy control can make the point of view of picking robot response smoothly to achieve the specified Angle , and there are no big over-shoot , which is good for cherry picking , reduce the breakage rate .

  17. Cultivar identification and genetic relationship of pineapple (Ananas comosus) cultivars using SSR markers.

    Science.gov (United States)

    Lin, Y S; Kuan, C S; Weng, I S; Tsai, C C

    2015-11-25

    The genetic relationships among 27 pineapple [Ananas comosus (L.) Merr.] cultivars and lines were examined using 16 simple sequence repeat (SSR) markers. The number of alleles per locus of the SSR markers ranged from 2 to 6 (average 3.19), for a total of 51 alleles. Similarity coefficients were calculated on the basis of 51 amplified bands. A dendrogram was created according to the 16 SSR markers by the unweighted pair-group method. The banding patterns obtained from the SSR primers allowed most of the cultivars and lines to be distinguished, with the exception of vegetative clones. According to the dendrogram, the 27 pineapple cultivars and lines were clustered into three main clusters and four individual clusters. As expected, the dendrogram showed that derived cultivars and lines are closely related to their parental cultivars; the genetic relationships between pineapple cultivars agree with the genealogy of their breeding history. In addition, the analysis showed that there is no obvious correlation between SSR markers and morphological characters. In conclusion, SSR analysis is an efficient method for pineapple cultivar identification and can offer valuable informative characters to identify pineapple cultivars in Taiwan.

  18. CULTIVAR RELEASE-IPR 98: Rust-resistant dwarf arabica coffee cultivar for dense spacing

    Directory of Open Access Journals (Sweden)

    Tumoru Sera

    2008-01-01

    Full Text Available The IPR 98 coffee cultivar is a dwarf plant smaller than the Catuaí Vermelho IAC-81 cultivar, completelyresistant to Hemileia vastatrix Berk. et Br. with medium precocity in ripening and superior quality and high yields with semidense,dense and super dense spacing in lower temperature coffee regions of the state of Paraná.

  19. Attitudes of ornamental trees and shrubs producers towards nursery production of ornamental beech cultivars in Serbia

    Directory of Open Access Journals (Sweden)

    Nonić Marina

    2016-01-01

    Full Text Available European beech (Fagus sylvatica L. is, along with its significance as a forest species, renowned as an ornamental species, due to its numerous cultivars. Ornamental beech cultivars are planted in various green spaces, but a small number of such trees have ascertained in Serbia. For the time being, production of beech cultivars is represented in a very small number of nurseries, with a negligible share of those seedlings in their total assortment. The aim of this research is to study the attitudes of ornamental trees and shrubs producers towards the nursery production of ornamental beech cultivars, and possibilities of its improvements in Serbia. “Door to door” survey and in-depth interviews were used as research techniques. Surveys with the representatives of 65 nurseries in Serbia (in the selected statistical region Šumadija and Western Serbia were conducted in the first stage of data collection. In the second stage of data collection were interviewed the representatives of the 10 nurseries who, during the survey, pointed out that they produce ornamental beech cultivars. Nurserymen’s attitudes suggest that there is a possibility to improve the production of ornamental beech cultivars in Serbia, with the appropriate support measures and increased interest of customers on the market, i.e. with the provision of subsidies for the production of seedlings and greater use of cultivars by utility companies in the cities of Serbia. [Projekat Ministarstva nauke Republike Srbije, br. ТP 31041: Establishment of forest plantations to increase the afforested areas in Serbia

  20. Simultaneous detection of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus using real-time PCR and high resolution melting analysis.

    Science.gov (United States)

    Komorowska, Beata; Fiore, Nicola; Zamorano, Alan; Li, Ruhui

    2014-08-01

    In this study, the real-time PCR assays were combined with high resolution melting (HRM) analysis for the simultaneous detection of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV) infection in sweet cherry trees. Detection of CNRMV and CGRMV was performed in a real-time PCR using a primer set for both of them or duplex real-time PCR that included one specific primer set for each virus. These two strategies allowed us to confirmed virus infection in all tested samples. In 17 field samples the technique revealed samples positive for CNRMV or CGRMV as well as positive for both viruses. In addition, the HRM analysis made it possible to differentiate clearly between CNRMV and CGRMV. Sequence variations among CNRMV and CGRMV isolates observed from the HRM peaks were confirmed by sequencing. To test the capability to use this method in field, forty one sweet cherry samples were examined by HRM analysis. The HRM data showed that seven samples were positive for CNRMV and three were infected with CGRMV. The results presented in this study indicated that real-time PCR followed by HRM analysis provides sensitive, automated and rapid tool to detect and differentiate between CNRMV and CGRMV isolates.

  1. The effect of the time and the budding method on the growth of young cherry trees cv. 'Łutówka'

    Directory of Open Access Journals (Sweden)

    Piotr Baryła

    2012-12-01

    Full Text Available The studies concerning the effect of the time and the methods of budding on the growth of young cherry trees were conducted in the years at Felin Experimental Farm of Lublin Agricultural University. The objects of investigations were the young cherry trees obtained as a result of budding of mahaleb cherry (Prunus mahaleb L. and sweet cherry (Prunus avium L. seedlings in the way by the chip budding-15th July and T-graft-15th July and 1st September. The used methods and the times of budding insignificantly affected the growth of young cherry trees cv. «Łutówka» in a nursery. There was showed that quality features of the trees were dependet on stock used type. Cherry trees obtained on mahaleb cherry were thicker, higher and better branched than on sweet cherry.

  2. Molybdenum supply and biological fixation of nitrogen by two Brazilian common bean cultivars

    Directory of Open Access Journals (Sweden)

    Alinne da Silva

    Full Text Available ABSTRACT The common bean has been considered to have low biological nitrogen fixation capacity; however, this process can be made more effective with molybdenum (Mo supplementation. The objective of this study was to evaluate the influence of Mo rates on the growth and biological nitrogen fixation by two Brazilian common bean cultivars using the 15N isotope dilution technique. The experiment was performed in 2014 in a completely randomized design arranged in a 5 x 3 factorial scheme, corresponding to 5 rates of Mo (control, 40, 80, 120 and 240 g ha-1, the common bean cultivars Aporé, Ouro Negro and NORH-54 (a non-nodulating common bean cultivar, and three replicates. The application of Mo and the inoculation with rhizobia strains contributed to improving nitrogen fixation and grain weight. The cultivar Ouro Negro showed a higher number and weight of nodules and a higher amount of nitrogen derived from the atmosphere than the cultivar Aporé. The biological nitrogen fixation of Aporé was more dependent on the application of Mo. These results indicated that inoculation with Rhizobium strains and Mo supply effectively contributed to biological nitrogen fixation and improving grain production.

  3. A quantum Cherry theorem for perturbations of the plane rotator

    Science.gov (United States)

    Barone, Fiorella; Graffi, Sandro

    2013-12-01

    We consider on L^2({T}^2) the Schrödinger operator family L_\\varepsilon : \\varepsilon in {R} with domain and action defined as D(L_\\varepsilon )=H^2({T}^2), L_\\varepsilon u=-1/2hbar ^2(α _1partial _{φ _1}^2+α _2partial _{φ _2}^2)u-ihbar (γ _1partial _{φ _1}+γ _2partial _{φ _2})u+\\varepsilon Vu. Here \\varepsilon in {R}, α = (α1, α2), γ = (γ1, γ2) are vectors of complex non-real frequencies, and V a pseudodifferential operator of order zero. Lɛ represents the Weyl quantization of the Hamiltonian family {L}_\\varepsilon (ξ,x)=1/2(α _1ξ _1^2+α _2ξ _2^2)+γ _1ξ _1+γ _2ξ _2+\\varepsilon {V}(ξ,x) defined on the phase space {R}^2× {T}^2, where {V}(ξ,x)in C^2({R}^2× {T}^2;{R}). We prove the uniform convergence with respect to ℏ ∈ [0, 1] of the quantum normal form, which reduces to the classical one for ℏ = 0. This result simultaneously entails an exact quantization formula for the quantum spectrum as well as a convergence criterion for the classical Birkhoff normal form generalizing a well known theorem of Cherry.

  4. CONVECTIVE DRYING OF CHERRY TOMATO: STUDY OF SKIN EFFECT

    Directory of Open Access Journals (Sweden)

    R. KHAMA

    2016-03-01

    Full Text Available A whole single cherry tomato was dried in a forced convective micro-dryer. The experiments were carried out at constant air velocity and humidity and temperatures of 50, 60, 70 °C. In order to study the effect of the skin, two sets of experiments were performed using a tomato with and without skin (easily removed. Shorter drying times were obtained when increasing drying temperatures as well as when removing sample skin. X-ray microtomography, a non-destructive 3D imaging technique was used to follow shrinkage of the samples. This phenomenon was introduced in the modelling part of this study. Analytical solutions of the Fick’law were used to determine the diffusion coefficient at the three temperatures studied, and then the activation energy was obtained through fitting the Arrhenius equation. The skin effect was clearly evidenced by showing that the mass transfer parameter values of an original tomato with skin were largely smaller than the one without skin. Indeed, the moisture effective diffusivity ranged from 2.56×10-11 to 7.67×10-11 m2·s-1 with activation energy of 50430 J·mol-1 for tomato with skin an ranged from 4.59×10-10 m2·s-1 to 6.73×10-10 m2·s-1 with activation energy of 17640 J.mol-1 for tomato without skin.

  5. Reproductive biology traits affecting productivity of sour cherry

    Directory of Open Access Journals (Sweden)

    Milica Fotiric Aksic

    2013-01-01

    Full Text Available The objective of this work was to evaluate variability in reproductive biology traits and the correlation between them in genotypes of 'Oblačinska' sour cherry (Prunus cerasus. High genetic diversity was found in the 41 evaluated genotypes, and significant differences were observed among them for all studied traits: flowering time, pollen germination, number of fruiting branches, production of flower and fruit, number of flowers per bud, fruit set, and limb yield efficiency. The number of fruiting branches significantly influenced the number of flower and fruit, fruit set, and yield efficiency. In addition to number of fruiting branches, yield efficiency was positively correlated with fruit set and production of flower and fruit. Results from principal component analysis suggested a reduction of the reproductive biology factors affecting yield to four main characters: number and structure of fruiting branches, flowering time, and pollen germination. Knowledge of the reproductive biology of the 'Oblačinska' genotypes can be used to select the appropriate ones to be grown or used as parents in breeding programs. In this sense, genotypes II/2, III/9, III/13, and III/14 have very good flower production and satisfactory pollen germination.

  6. Mapping of Candidate Genes Involved in Bud Dormancy and Flowering Time in Sweet Cherry (Prunus avium).

    Science.gov (United States)

    Castède, Sophie; Campoy, José Antonio; Le Dantec, Loïck; Quero-García, José; Barreneche, Teresa; Wenden, Bénédicte; Dirlewanger, Elisabeth

    2015-01-01

    The timing of flowering in perennial plants is crucial for their survival in temperate climates and is regulated by the duration of bud dormancy. Bud dormancy release and bud break depend on the perception of cumulative chilling during endodormancy and heat during the bud development. The objectives of this work were to identify candidate genes involved in dormancy and flowering processes in sweet cherry, their mapping in two mapping progenies 'Regina' × 'Garnet' and 'Regina' × 'Lapins', and to select those candidate genes which co-localized with quantitative trait loci (QTLs) associated with temperature requirements for bud dormancy release and flowering. Based on available data on flowering processes in various species, a list of 79 candidate genes was established. The peach and sweet cherry orthologs were identified and primers were designed to amplify sweet cherry candidate gene fragments. Based on the amplified sequences of the three parents of the mapping progenies, SNPs segregations in the progenies were identified. Thirty five candidate genes were genetically mapped in at least one of the two progenies and all were in silico mapped. Co-localization between candidate genes and QTLs associated with temperature requirements and flowering date were identified for the first time in sweet cherry. The allelic composition of the candidate genes located in the major QTL for heat requirements and flowering date located on linkage group 4 have a significant effect on these two traits indicating their potential use for breeding programs in sweet cherry to select new varieties adapted to putative future climatic conditions.

  7. Tart cherry anthocyanins suppress inflammation-induced pain behavior in rat.

    Science.gov (United States)

    Tall, Jill M; Seeram, Navindra P; Zhao, Chengshui; Nair, Muraleedharan G; Meyer, Richard A; Raja, Srinivasa N

    2004-08-12

    The use of complementary and alternative medicine (CAM) has increased in the United States and more patients are seeking CAM therapies for control of pain. The present investigation tested the efficacy of orally administered anthocyanins extracted from tart cherries on inflammation-induced pain behavior in rats. Paw withdrawal latency to radiant heat and paw withdrawal threshold to von Frey probes were measured. The first set of experiments examined the effects of tart cherry anthocyanins (400 mg/kg) on the nociceptive behaviors and edema associated with inflammation induced by intraplantar injection of 1% carrageenan. These studies also included tests of motor coordination. The second set of experiments determined if tart cherry anthocyanins (15, 85, and 400 mg/kg) dose-dependently affected the inflammation induced by intraplantar injection of 25% complete Freund's adjuvant. We found that tart cherry extracts reduce inflammation-induced thermal hyperalgesia, mechanical hyperalgesia and paw edema. The suppression of thermal hyperalgesia was dose-dependent and the efficacy of highest dose (400 mg/kg) was similar to indomethacin (5 mg/kg). The highest dose anthocyanin (400 mg/kg) had no effects on motor function. These data suggest that tart cherry anthocyanins may have a beneficial role in the treatment of inflammatory pain. The antihyperalgesic effects may be related to the anti-inflammatory and antioxidant properties of anthocyanins. A better understanding of the modulatory role of dietary constituents and phytonutrients on pain will offer further therapeutic options for treating patients with persistent and chronic pain conditions.

  8. Rendimento de cultivares de tomate para processamento em Goias Yield of processing tomato cultivars in Goiás State, Brazil

    Directory of Open Access Journals (Sweden)

    Nei Peixoto

    1999-03-01

    began 35 to 41 days after sowing, with the harvesting period starting 38 days later. Cultivars Petomech, IPA 6, and Improved Topmech, followed by IPA 5 and Agrocica 72, were high yielding and showed a high fruit firmness. Cultivars Roma VFN, Rossol and Agrocica 08, were also high yielding but produced fruits unsuitable for long distance transportation. With the exception of cultivar Itaparica, which showed the best adaptation to less favourable enviroments, all genotypes were responsive to enviroment improvement. Higher stability to environmental variation was observed in cultivars Agrocica 33, Calmec VF, IPA 6, Rio Fuego, Santa Adélia, Topmec Melhorado, and UC 82.

  9. Chilling and host plant/site associated eclosion times of western cherry fruit fly (Diptera:Tephritidae) and a host-specific parasitoid

    Science.gov (United States)

    The western cherry fruit fly, Rhagoletis indifferens Curran (Diptera: Tephritidae), is native to bitter cherry, Prunus emarginata (Douglas ex Hooker) Eaton, but ~100 years ago established on earlier-fruiting domesticated sweet cherry, Prunus avium (L.) L. Here, we determined if eclosion times of ad...

  10. First Report of a New Phytoplasma Subgroup, 16SrIII-S, Associated with Decline Disease Affecting Sweet and Sour Cherry Trees

    Science.gov (United States)

    During July 2007, we observed sweet cherry (Prunus avium) and sour cherry (Prunus cerasus) trees exhibiting disease symptoms suggestive of possible phytoplasma infection in a large orchard in the Kaunas region of Lithuania. Samples of leaf tissue were collected from sweet cherry trees that were aff...

  11. First report of sweet cherry virescence disease in China and its association with infection by a ‘Candidatus Phytoplasma ziziphi’-related strain

    Science.gov (United States)

    Sweet cherry (Prunus avium L.) is a deciduous tree originating in the Black Sea/Caspian Sea region where Asia and Europe converge. Being highly valued for its timber and fruit, sweet cherry has been cultivated and naturalized on all continents. Over the past decade, the area of sweet cherry culti...

  12. Temperature-mediated kill and oviposition of Western Cherry Fruit Fly (Diptera: Tephritidae) in the presence of Spinosad

    Science.gov (United States)

    Western cherry fruit fly, Rhagoletis indifferens Curran (Diptera: Tephritidae), is a quarantine pest of sweet cherry (Prunus avium (L.) L.) that is managed using insecticides, including spinosad, an organic compound that can be applied in low spray volumes. Identifying factors that can increase the...

  13. 76 FR 61340 - Notice of Decision To Authorize the Importation of Fresh Apricot, Sweet Cherry, and Plumcot Fruit...

    Science.gov (United States)

    2011-10-04

    ... Apricot, Sweet Cherry, and Plumcot Fruit From South Africa Into the Continental United States AGENCY... cherry, and plumcot fruit from South Africa. Based ] on the findings of a pest risk analysis, which we... introducing or disseminating plant pests or noxious weeds via the importation of fresh apricot, sweet...

  14. The Effect of the Cherry Hill Study Skills Program on Eighth Grade Students' Reading Comprehension and Study Skills.

    Science.gov (United States)

    La Marca, Marilyn Tierney

    A study was conducted to determine the effects of the "Cherry Hill Study Skills Program" on eighth grade students' reading comprehension and study skills. The "Cherry Hill Study Skills Program" is a process oriented course dealing with the sequential development of nine specific skills deemed essential to the retrieval and retention of information…

  15. Reduction in Emergence of Rhagoletis indifferens (Diptera: Tephritidae) from Sweet Cherries with Different Egg and Larval Distributions Using Newer Insecticides

    Science.gov (United States)

    Western cherry fruit fly, Rhagoletis indifferens Curran (Diptera: Tephritidae), is the major insect pest of sweet cherry, Prunus avium (L.) L., in the Pacific Northwest of the U.S. To reduce fly populations in unharvested fruit following the completion of commercial harvest, it is important to cont...

  16. Soil moisture and relative humidity effects during post-diapause on emergence of western cherry fruit fly (Diptera: Tephritidae)

    Science.gov (United States)

    Western cherry fruit fly, Rhagoletis indifferens Curran, is a pest of sweet cherry, Prunus avium (L.) L., in western North America that is found in relatively moist and dry habitats. In this study, fly pupae from Kennewick and Roslyn in Washington state, U.S.A., were used to test the hypotheses tha...

  17. Behavioral responses, rate of mortality, and oviposition of western cherry fruit fly exposed to Malathion, Zeta-cypermethrin, and Spinetoram

    Science.gov (United States)

    Western cherry fruit fly, Rhagoletis indifferens Curran (Diptera: Tephritidae), is a pest of sweet and tart cherry, Prunus avium L. (L.) and P. cerasus L., respectively, in western North America. This fly is commonly controlled with spinosad bait sprays, but these sprays are ineffective against sp...

  18. Pupal Mortality and Adult Emergence of Western Cherry Fruit Fly (Diptera: Tephritidae) Exposed to the Fungus Muscodor albus (Xylariales: Xylariaceae)

    Science.gov (United States)

    Western cherry fruit fly, Rhagoletis indifferens Curran, is a major pest of sweet cherry, Prunus avium (L.) L., that is conventionally controlled using insecticides. One alternative to the use of insecticides for fly control could be fumigation of the fly’s overwintering habitat using the fungus Mus...

  19. 75 FR 31719 - Tart Cherries Grown in the States of Michigan, New York, Pennsylvania, Oregon, Utah, Washington...

    Science.gov (United States)

    2010-06-04

    ...; ] DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 930 Tart Cherries Grown in the States of... handling of tart cherries grown in Michigan, New York, Pennsylvania, Oregon, Utah, Washington, and... with respect to the proposed amendments to Marketing Order 930 regulating the handling of tart...

  20. Inhibitory Effects of Tart Cherry (Prunus cerasus) Juice on Xanthine Oxidoreductase Activity and its Hypouricemic and Antioxidant Effects on Rats.

    Science.gov (United States)

    Haidari, F; Mohammad Shahi, M; Keshavarz, S A; Rashidi, M R

    2009-03-01

    The aim of this study was to investigate the effect of tart cherry juice on serum uric acid levels, hepatic xanthine oxidoreductase activity and two non-invasive biomarkers of oxidative stress (total antioxidant capacity and malondialdehyde concentration), in normal and hyperuricemic rats. Tart cherry juice (5 ml/kg) was given by oral gavage to rats for 2 weeks. Allopurinol (5 mg/kg) was used as a positive control and was also given by oral gavage. Data showed that tart cherry juice treatment did not cause any significant reduction in the serum uric acid levels in normal rats, but significantly reduced (PTart cherry juice treatment also inhibited hepatic xanthine oxidase/dehydrogenase activity. Moreover, a significant increase (Ptart cherry juice treated-rats in both normal and hyperuricemic groups. The oral administration of tart cherry juice also led to a significant reduction (Ptart cherry, it could not significantly change anti-oxidative parameters. These features of tart cherry make it an attractive candidate for the prophylactic treatment of hyperuricaemia, particularly if it is to be taken on a long-term basis. Further investigations to define its clinical efficacy would be highly desirable.

  1. Molecular typing of Dutch isolates of Xanthomonas arboricola pathovar pruni isolated from ornamental cherry laurel.Journal of Plant Pathology

    NARCIS (Netherlands)

    Bergsma-Vlami, M.; Martin, W.; Koenraadt, H.; Teunissen, H.; Pothier, J.F.; Duffy, B.; Doorn, van J.

    2012-01-01

    Xanthomonas arboricola pv. pruni (Xap) has been found in several cherry laurel (Prunus laurocerasus) nurseries in the Netherlands, causing leaf spot. As no information is available yet about the epidemiology of this quarantine bacterium in cherry laurel, molecular typing of Xap isolates can consider

  2. Inter-Simple Sequence Repeat (ISSR Markers to Study Genetic Diversity Among Cotton Cultivars in Associated with Salt Tolerance

    Directory of Open Access Journals (Sweden)

    Ali Akbar ABDI

    2012-11-01

    Full Text Available Developing salt-tolerant crops is very important as a significant proportion of cultivated land is salt-affected. Screening and selection of salt tolerant genotypes of cotton using DNA molecular markers not only introduce tolerant cultivars useful for hybridization and breeding programs but also detect DNA regions involved in mechanism of salinity tolerance. To study this, 28 cotton cultivars, including 8 Iranian cotton varieties were grown in pots under greenhouse condition and three salt treatments were imposed with salt solutions (0, 70 and 140 mM NaCl. Eight agronomic traits including root length, root fresh weight, root dry weight, chlorophyll and fluorescence index, K+ and Na+ contents in shoot (above ground biomass, and K+/Na+ ratio were measured. Cluster analysis of cultivars based on measured agronomic traits, showed �Cindose� and �Ciacra� as the most tolerant cultivars, and �B-557� and �43347� as the most sensitive cultivars of salt damage. A total of 65 polymorphic DNA fragments were generated at 14 inter-simple sequence repeat (ISSR loci. Plants of 28 cultivars of cotton grouped into three clusters based on ISSR markers. Regression analysis of markers in relation with traits data showed that 23, 33 and 30 markers associated with the measured traits in three salt treatments respectively. These markers might help breeders in any marker assisted selection program in order to improving cotton cultivars against salt stress.

  3. New Cultivars Derived from Crosses between Commercial Cultivar and a Wild Population of Papaya Rescued at Its Center of Origin

    OpenAIRE

    Mariela Vázquez Calderón; Manuel Jesús Zavala León; Fernando Amilcar Contreras Martín; Francisco Espadas y Gil; Abelardo Navarrete Yabur; Lorenzo Felipe Sánchez Teyer; Jorge M. Santamaría

    2014-01-01

    In order to generate new varieties, this study focused on the rescue and use of landraces and wild Carica papaya L. populations located at southern Yucatan, Mexico, to cross them with a commercial papaya cultivar (Maradol). In the cross L7 × M22, The native parent line L7 was used as the receiver parent while the commercial Maradol (M22) was used as the donor parent, seeking to generate genotypes with improved productivity and reduced plant height. Cluster analysis and principal components an...

  4. Seed Vigor and Field Performance of Winter Oilseed Rape (Brassica napus L. Cultivars

    Directory of Open Access Journals (Sweden)

    Kazem GHASSEMI-GOLEZANI

    2010-12-01

    Full Text Available The effect of seed vigor on field performance of two oilseed rape cultivars (Licord and Okapi was investigated in 2008 at the Research Farm of the University Tabriz, Iran. The experiment was arranged as factorial based on RCB design with three replications. A sub-sample of seeds of each cultivar was kept as control. The other two sub-samples of each cultivar with about 15% moisture content were artificially deteriorated at 40C for 9 and 12 days (Licord and 7 and 10 days (Okapi. Consequently, three seed lots for each cultivar with different levels of vigor were provided. The results showed that the mean emergence time increased with decreasing seed vigor. However, the highest percentage of seedling emergence was obtained for the high quality seed lot and decreased with decreasing seed lot vigor. Decreasing seed vigor led to significant reduction in ground cover of both cultivars; mainly due to poor stand establishment. Ground cover of Licord was higher than that of Okapi. Plants from high vigor seed lots of both cultivars had higher leaf chlorophyll content index at early stages of growth, compared with those from low quality seed lots. Grains per plant, 1000 grain weight and grain yield per plant for plants from deteriorated seed lots were higher than those from high-vigor seed lot. However, these advantages in individual plant performance were not sufficient to compensate for low stand establishment. Consequently, grain yield per unit area significantly improved with increasing seed vigor of oilseed rape cultivars, even within the range of acceptable germination.

  5. Transgene Stacking in Cotton Improvement

    Institute of Scientific and Technical Information of China (English)

    YANG Ye-hua; WANG Xue-kui; YAO Ming-jing; FAN Yu-peng; GAO Da-yu

    2008-01-01

    @@ To date,more and more transgenic varieties of upland cotton (Gossypium hirsuturn L.) generated with transgenes,which derived from varies of alien species,are playing important role in agricultural production.Stacking of multi-transgenes has a potential for combining all the merits of distinct transgenic lines in a cultivar and possibly makes a significant contribution to cultivar improvement.

  6. Cadmium accumulation in different pakchoi cultivars and screening for pollution-safe cultivars

    Institute of Scientific and Technical Information of China (English)

    Ying CHEN; Ting-qiang LI; Xuan HAN; Zhe-li DING; Xiao-e YANG; Ye-fei JIN

    2012-01-01

    The selection and breeding of pollution-safe cultivars (PSCs) is a practicable and cost-effective approach to minimize the influx of heavy metal to the human food chain.In this study,both pot-culture and field experiments were conducted to identify and screen out cadmium pollution-safe cultivars (Cd-PSCs) from 50 pakchoi (Brassica rapa L.ssp.chinensis) cultivars for food safety.When treated with 1.0 or 2.5 mg/kg Cd,most of the pakchoi cultlvars (>70%)showed greater or similar shoot biomass when compared with the control.This result indicates that pakchoi has a considerable tolerance to soil Cd stress.Cd concentrations in the shoot varied significantly (P<0.05) between cultivars:in two Cd treatments (1.0 and 2.5 mg/kg),the average values were 0.074 and 0.175 mg/kg fresh weight (FW),respectively.Cd concentrations in the shoots of 14 pakchoi cultivars were lower than 0.05 mg/kg FW.In pot-culture experiments,both enrichment factors (EFs) and translocation factors (TFs) of six pakchoi cultivars were lower than 1.0.The field studies further confirmed that the Hangzhouyoudonger,Aijiaoheiye 333,and Zaoshenghuajing cultivars are Cd-PSCs,and are therefore suitable for growth in low Cd-contaminated soils (≤1.2 mg/kg) without any risk to food safety.

  7. Capacidade produtiva de cultivares de berinjela

    Directory of Open Access Journals (Sweden)

    Antonini Antonio C. C.

    2002-01-01

    Full Text Available Determinou-se a capacidade produtiva de diferentes híbridos e cultivares de berinjela. Sete híbridos (Nápoli, Diamante Negro, Ciça, F-100, Super F-100, Kiko e F-2000 e duas cultivares de polinização aberta (Suzuki e Florida Market foram avaliados. O delineamento experimental foi em blocos ao acaso, constituído de nove tratamentos e três repetições. Cada repetição foi composta de oito plantas. As mudas foram plantadas no espaçamento 1,5m x 1,0m. A cultivar Suzuki produziu a maior quantidade de frutos por planta (27 frutos embora tenha apenas diferido significativamente da cultivar Flórida Market (13 frutos. Os híbridos testados apresentaram número de frutos variando de 20 a 25. Não foi verificada diferença significativa para o número de frutos de 1ª e 2ª categoria entre os materiais testados. Flórida Market e Kiko apresentaram a maior massa média do fruto (362,5 e 358,7 g, respectivamente, diferindo significativamente da cultivar Suzuki, que produziu frutos com a menor massa (178,9 g. As maiores produções por planta foram obtidas com o híbrido Kiko que diferiu significativamente da cultivar Suzuki. Foi verificado que os híbridos Kiko, Diamante Negro, Ciça e F-2000 apresentam potencial de produção semelhante ao Nápoli, atual padrão de mercado.

  8. HPLC-Q-TOF-MS identification of antioxidant and antihypertensive peptides recovered from cherry (Prunus cerasus L.) subproducts.

    Science.gov (United States)

    García, María Concepción; Endermann, Jochan; González-García, Estefanía; Marina, María Luisa

    2015-02-11

    The processing of fruits, such as cherries, is characterized by generating a lot of waste material such as fruit stones, skins, etc. To contribute to environmental sustainability, it is necessary to recover these residues. Cherry stones contain seeds with a significant amount of proteins that are underused and undervalued. The aim of this work was to extract cherry seed proteins, to evaluate the presence of bioactive peptides, and to identify them by mass spectrometry. The digestion of cherry seed proteins was optimized, and three different enzymes were employed: Alcalase, Thermolysin, and Flavourzyme. Peptide extracts obtained by the digestion of the cherry seed protein isolate with Alcalase and Thermolysin yielded the highest antioxidant and antihypertensive capacities. Ultrafiltration of hydrolysates allowed obtaining fractions with high antioxidant and antihypertensive capabilities. HPLC-Q-TOF-MS together with bioinformatics tools enabled one to identify peptides in these fractions.

  9. In-package atmospheric pressure cold plasma treatment of cherry tomatoes.

    Science.gov (United States)

    Misra, Nrusimha Nath; Keener, Kevin M; Bourke, Paula; Mosnier, Jean-Paul; Cullen, Patrick J

    2014-08-01

    Cold plasma is increasingly under research for decontamination of foods, especially fresh fruits and vegetables. The effect of cold plasma on food quality, however, remains under researched. This study investigates the effects of cold plasma generated within a sealed package from a dielectric barrier discharge on the physical quality parameters and respiration rates of cherry tomatoes. Respiration rates and weight loss were monitored continuously, while other parameters are reported at the end of storage period. Differences among weight loss, pH and firmness for control and treated cherry tomatoes were insignificant towards the end of storage life. Changes in respiration rates and colour of tomatoes were recorded as a function of treatment, which were not drastic. The results implicate that cold plasma could be employed as a means for decontamination of cherry tomatoes while retaining product quality.

  10. Analysis of wild sweet cherry (Prunus avium L. germplasm diversity in south-east Serbia

    Directory of Open Access Journals (Sweden)

    Mratinić Evica

    2012-01-01

    Full Text Available Ten wild growing sweet cherry (Prunus avium L. genotypes from South-East Serbia with different fruit skin color were analyzed for its phenological, morphological and chemical traits. Agronomic evaluation of germplasm accessions revealed considerable diversity among different accessions for all the characters studied. The analysis of variance revealed significant differences among all genotypes for almost all examined properties. Cluster analysis showed adequate grouping of wild sweet cherry genotypes according to pomological characterization and distinguished them into two distinct groups. The first group had two subgroups and consisted of seven genotypes, while the second one included only three accessions. Despite of the significant differences among genotypes, the total concentration of phenols made a clear separation between the clusters. The level of genetic diversity in these wild sweet cherry genotypes is very high and therefore these trees are useful sources of variability for attributes studied and can be employed in further breeding programs or conservation.

  11. Optimal Fluorescence Waveband Determination for Detecting Defective Cherry Tomatoes Using a Fluorescence Excitation-Emission Matrix

    Directory of Open Access Journals (Sweden)

    In-Suck Baek

    2014-11-01

    Full Text Available A multi-spectral fluorescence imaging technique was used to detect defective cherry tomatoes. The fluorescence excitation and emission matrix was used to measure for defects, sound surface and stem areas to determine the optimal fluorescence excitation and emission wavelengths for discrimination. Two-way ANOVA revealed the optimal excitation wavelength for detecting defect areas was 410 nm. Principal component analysis (PCA was applied to the fluorescence emission spectra of all regions at 410 nm excitation to determine the emission wavelengths for defect detection. The major emission wavelengths were 688 nm and 506 nm for the detection. Fluorescence images combined with the determined emission wavebands demonstrated the feasibility of detecting defective cherry tomatoes with >98% accuracy. Multi-spectral fluorescence imaging has potential utility in non-destructive quality sorting of cherry tomatoes.

  12. CULTIVAR RELEASE-IAC-Alvorada and IAC-Diplomata: new common bean cultivars

    Directory of Open Access Journals (Sweden)

    Sérgio Augusto Morais Carbonell

    2008-01-01

    Full Text Available The aim of common bean breeding programs is the development of high-yielding cultivars, with multipledisease resistance and high technological and nutritional grain quality. Two cultivars, IAC-Alvorada with carioca grain, andIAC-Diplomata with black grain, meet these standards, according to the results of trials of Value for Cultivation and Use(VCU 2005/2006/2007 in São Paulo. The cultivars, developed by the Instituto Agronômico de Campinas, were registered bythe MAPA/RNC.

  13. Energy and economic analysis of sweet cherry production in Turkey: A case study from Isparta province

    Energy Technology Data Exchange (ETDEWEB)

    Demircan, Vecdi [Faculty of Agriculture, Department of Agricultural Economics, Suleyman Demirel University, Isparta 32260 (Turkey); Ekinci, Kamil [Faculty of Agriculture, Department of Agricultural Machinery, Suleyman Demirel University, Isparta 32260 (Turkey)]. E-mail: kekinci@ziraat.sdu.edu.tr; Keener, Harold M. [Department of Food, Agricultural, and Biological Engineering, Ohio Agricultural Research Centre, The Ohio State University, Wooster, Ohio 44691 (United States); Akbolat, Davut [Faculty of Agriculture, Department of Agricultural Machinery, Suleyman Demirel University, Isparta 32260 (Turkey); Ekinci, Caglar [Faculty of Civil Engineering, Infrastructure and Environment Department, Bauhaus University (Germany)

    2006-08-15

    A survey was conducted using a face to face questionnaire with 92 sweet cherry (Prunus avium L.) producers from 10 villages in five districts of the Isparta province where there is intensive sweet cherry production. The data collected was analyzed for the energy and economics of sweet cherry production. The results showed that the most energy consuming input for the different operations investigated was chemical fertilisers (45.35%), especially nitrogen (38.05%). The energy consumption for Diesel fuel was 21.53% of the total energy input. Although chemicals for plant protection had a small portion (1.45%) of the total energy input, the use of pesticide in sweet cherry production per hectare in the Isparta province was 5.36 times higher than that of Turkey's average, increasing the environmental risk problem. The energy use efficiency, defined as energy produced per unit of energy used, was 1.23. The specific energy of sweet cherry production was determined to be 3163.43 MJ tonnes{sup -1}. It was found that the direct and indirect energy inputs were 34.48% and 54.91% of the total energy input, respectively. Among the inputs, renewable energy sources constituted 16.34% of the total energy input, which was lower than that of the non-renewable resources (chemical fertilisers and Diesel fuel). The results showed that the net return from sweet cherry production in the surveyed farms was satisfactory, as demonstrated by the benefit-cost ratio of 2.53 calculated by dividing the gross value of production by the total cost of production per hectare.

  14. Changes of Aroma Components in Hongdeng Sweet Cherry During Fruit Development

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    To study the changes of aroma components in sweet cherry during fruit development, the aroma components in sweet cherry fruit from the green stage, the color stage, the commercial stage, and the ripe stage were collected using head-space solid phase microextraction (HS-SPME), and were analyzed using a gas chromatograph-mass spectrophotometer (GCMS). A total of 37 compounds were identified from the sample extracts. Aldehydes, alcohols, and esters were the major constituents. The aroma components behaved differently during the fruit developmental period. C6 aldehydes and aromatic aldehydes were the main aldehydes in the sweet cherry fruit. The contents of C6 aldehydes increased quickly to 84.16% in the color stage, then decreased as ripening proceeded, and then, the contents decreased to 59.20 and 55.58% at the commercial stage and the ripe stage, respectively. The aromatic aldehydes (benzaldehyde) increased as ripening proceeded, and the maximum was found at the ripe stage. Alcohols of sweet cherry fruit included C6 alcohols and aromatic alcohols. The content of (E)-2-hexen-1-ol increased as ripening proceeded. The maximum was found at the commercial stage; alcohol was only found at the ripe stage. Ester components included ethyl acetate, butanoic acid ethyl ester,hexanoic acid ethyl ester, which increased as the fruit ripened. Hexanal, (E)-2-hexenal, benzaldehyde, (E)-2-hexen-1-ol,ethyl acetate, and hexanoic acid ethyl ester were the characteristic aroma components of sweet cherry fruit. These aroma components started to form drastically at the color stage, and the main aroma was formed at the commercial stage, which then turned bad at the ripe stage because of the presence of alcohol. So the optimal harvest time of sweet cherry was at the commercial stage.

  15. Water stress augments silicon-mediated resistance of susceptible sugarcane cultivars to the stalk borer Eldana saccharina (Lepidoptera: Pyralidae).

    Science.gov (United States)

    Kvedaras, O L; Keeping, M G; Goebel, F-R; Byrne, M J

    2007-04-01

    Silicon (Si) can improve resistance of plants to insect attack and may also enhance tolerance of water stress. This study tested if Si-mediated host plant resistance to insect attack was augmented by water stress. Four sugarcane cultivars, two resistant (N21, N33) and two susceptible (N26, N11) to Eldana saccharina Walker were grown in a pot trial in Si-deficient river sand, with (Si+) and without (Si-) calcium silicate. To induce water stress, irrigation to half the trial was reduced after 8.5 months. The trial was artificially infested with E. saccharina eggs after water reduction and harvested 66 days later. Silicon treated, stressed and non-stressed plants of the same cultivar did not differ appreciably in Si content. Decreases in numbers of borers recovered and stalk damage were not associated with comparable increases in rind hardness in Si+ cane, particularly in water-stressed susceptible cultivars. Overall, Si+ plants displayed increased resistance to E. saccharina attack compared with Si- plants. Borer recoveries were significantly lower in stressed Si+ cane compared with either stressed Si- or non-stressed Si- and Si+ cane. Generally, fewer borers were recovered from resistant cultivars than susceptible cultivars. Stalk damage was significantly lower in Si+ cane than in Si- cane, for N21, N11 and N26. Stalk damage was significantly less in Si+ combined susceptible cultivars than in Si- combined susceptible cultivars under non-stressed and especially stressed conditions. In general, the reduction in borer numbers and stalk damage in Si+ plants was greater for water-stressed cane than non-stressed cane, particularly for susceptible sugarcane cultivars. The hypothesis that Si affords greater protection against E. saccharina borer attack in water-stressed sugarcane than in non-stressed cane and that this benefit is greatly enhanced in susceptible cultivars is supported. A possible active role for soluble Si in defence against E. saccharina is proposed.

  16. Production of strawberry cultivars in closed hydroponic systems and coconut fibre substrate

    Directory of Open Access Journals (Sweden)

    Fabio Rodrigues de Miranda

    Full Text Available The objective of this work was to evaluate yield and average fruit weight of strawberry cultivars Albion, Camarosa, Festival and Oso Grande, in two closed hydroponic systems (gutters and grow bags, using coconut fibre as substrate. The experimental design was of randomised blocks, divided into strips, with five replications. The hydroponic systems did not differ significantly as to yield, with advantages, such as savings in water and fertilizer and reduced environmental impact, over open systems. The most productive cultivar was Festival, followed by Oso Grande, with average yields of 6.99 kg m-2 and 5.56 kg m-2 respectively. The cultivars with the greatest fruit weight were Oso Grande and Albion, having averages of 11.8 and 11.1 g respectively, with the former being significantly superior to the latter. The highest yield (7.4 kg m-2 was obtained from the cultivar Festival under the gutter system. The Ibiapaba region has conditions which are favourable to strawberry production in relation to precocity (harvesting starts in the 6th week of growth and production continues throughout the year; however there is a need to test new cultivars and to improve the cultivation techniques with an aim to producing larger-sized fruit.

  17. Cherry Featured in NCI’s Spotlight on Scientists Video Series | Poster

    Science.gov (United States)

    James Cherry, Ph.D., learned at an early age that education is crucial to success. He credits his mentors, some of whom include his grandmother, Shepherd University professor Burton Lidgerding, Ph.D., David Munroe, Ph.D., Frederick National Lab, and Robert J. Hohman, Ph.D., National Institute of Allergy and Infectious Diseases, for guiding him to the career he has today. Cherry, scientific program director, Office of Scientific Operations (OSO), NCI at Frederick, is one of the scientists featured in NCI’s Spotlight on Scientists video series.

  18. Tetraquark resonances, flip-flop and cherry in a broken glass model

    CERN Document Server

    Bicudo, P; Cardoso, N

    2010-01-01

    We develop a formalism to study tetraquarks using the generalized flip-flop potential, which include the tetraquark potential component. Technically this is a difficult problem, needing the solution of the Schr\\"odinger equation in a multidimensional space. Since the tetraquark may at any time escape to a pair of mesons, here we study a simplified two-variable toy model and explore the analogy with a cherry in a glass, but a broken one where the cherry may escape from. We also compute the decay width in this two-variable picture, solving the Schr\\"odinger equation for the outgoing spherical wave.

  19. Solar heating and hot water system installed at Cherry Hill, New Jersey. [Hotels

    Energy Technology Data Exchange (ETDEWEB)

    1979-05-16

    The solar heating and hot water system installed in existing buildings at the Cherry Hill Inn in Cherry Hill, New Jersey is described in detail. The system went into operation November 8, 1978 and is expected to furnish 31.5% of the overall heating load and 29.8% of the hot water load. The collectors are General Electric Company liquid evacuated tube type. The storage system is an above ground insulated steel water tank with a capacity of 7,500 gallons.

  20. A Belated Green Revolution for Cannabis: Virtual Genetic Resources to Fast-Track Cultivar Development

    OpenAIRE

    Matthew Timothy Welling; Timothy Shapter; Terry James Rose; Lei Liu; Rhia Stanger; Graham John King

    2016-01-01

    Cannabis is a predominantly diecious phenotypically diverse domesticated genus with few if any extant natural populations. International narcotics conventions and associated legislation have constrained the establishment, characterization, and use of Cannabis genetic resource collections. This has resulted in the underutilization of genepool variability in cultivar development and has limited the inclusion of secondary genepools associated with genetic improvement strategies of the Green Revo...

  1. ‘BRS MAGNA’ – a novel grape cultivar for juice making, with wide climatic adaptation

    Directory of Open Access Journals (Sweden)

    Patricia Ritschel

    2014-11-01

    Full Text Available ‘BRS Magna’ is a-novel cultivar to make grape juice, which presents intermediate productive cycle and wide climatic adaptation, released as an alternative to improve the color, the sweetness and the flavor of grape juice in Brazil.

  2. Response of broccoli and cabbage hybrid cultivars to clomazone

    Science.gov (United States)

    Clomazone herbicide (Command 3ME) is registered for cabbage in the U.S., but not for other cultivar groups within Brassica oleracea. Cabbage cultivars vary in clomazone tolerance, and recommended use rates can cause severe foliar chlorosis and yield reduction to susceptible cultivars. The objectiv...

  3. Distinguishing Nonpareil marketing group almond cultivars through multivariate analyses.

    Science.gov (United States)

    Ledbetter, Craig A; Sisterson, Mark S

    2013-09-01

    More than 80% of the world's almonds are grown in California with several dozen almond cultivars available commercially. To facilitate promotion and sale, almond cultivars are categorized into marketing groups based on kernel shape and appearance. Several marketing groups are recognized, with the Nonpareil Marketing Group (NMG) demanding the highest prices. Placement of cultivars into the NMG is historical and no objective standards exist for deciding whether newly developed cultivars belong in the NMG. Principal component analyses (PCA) were used to identify nut and kernel characteristics best separating the 4 NMG cultivars (Nonpareil, Jeffries, Kapareil, and Milow) from a representative of the California Marketing Group (cultivar Carmel) and the Mission Marketing Group (cultivar Padre). In addition, discriminant analyses were used to determine cultivar misclassification rates between and within the marketing groups. All 19 evaluated carpological characters differed significantly among the 6 cultivars and during 2 harvest seasons. A clear distinction of NMG cultivars from representatives of the California and Mission Marketing Groups was evident from a PCA involving the 6 cultivars. Further, NMG kernels were successfully discriminated from kernels representing the California and Mission Marketing Groups with overall kernel misclassification of only 2% using 16 of the 19 evaluated characters. Pellicle luminosity was the most discriminating character, regardless of the character set used in analyses. Results provide an objective classification of NMG almond kernels, clearly distinguishing them from kernels of cultivars representing the California and Mission Marketing Groups.

  4. Optimization of durum wheat bread from a selenium-rich cultivar fortified with bran.

    Science.gov (United States)

    Previtali, Maria Assunta; Mastromatteo, Marcella; Conte, Amalia; De Vita, Pasquale; Ficco, Donatella Bianca Maria; Del Nobile, Matteo Alessandro

    2016-02-01

    In this work the effect of bran addition (5 %, 10 %, 15 %, 20 %, 25 %, 30 %) on sensory, nutritional and mechanical properties of bread made from a durum wheat semolina enriched with selenium (cultivar PR22D89) was addressed; traditional and whole-meal bread from the same cultivar PR22D89, without any further bran addition, were also investigated for comparative purpose. In order to improve the durum wheat functional bread, different structuring agents (agar agar, gellan gum, guar seed flour, hydroxy-propyl-cellulose, modified food starch-CAPSUL® and tapioca starch) were firstly screened and then optimized. Sensory, textural and nutritional properties of bread were studied in each step. Results showed that bread from PR22D89 cultivar with addition of bran up to 30 % was completely accepted from the textural, nutritional and sensory points of view with proper utilization of guar seed flour or modified food-starch (2 %).

  5. Yield and quality of maize stover:Variation among cultivars and effects of N fertilization

    Institute of Scientific and Technical Information of China (English)

    LIANG Ming-yuan; WANG Gui-yan; LIANG Wei-li; SHI Peng-fei; DANG Jing; SUI Peng; HU Chun-sheng

    2015-01-01

    Biomass yields and concentrations of crude protein (CP), ether extract (EE), neutral detergent ifber (NDF), acid detergent ifber (ADF), and crude ifber (CF) were analyzed for ifve cultivars of summer-sown maize (Zea mays L.) stover grown in ifeld trials at three rates of N fertilization, and sampled immediately after grain harvest. The results revealed differences in yields and concentrations of nutrients according to stalk height and hence harvest portion among the cultivars. N application greatly increased biomass yield and CP, especial y in upper stalks and to a lesser extent, EE. Concentrations of NDF and ADF decreased as N rate increased. The results show that stovers from al local popular maize cultivars are suitable as animal fodder and that moderate N application improves feed quality of stover.

  6. Nontargeted metabolite profiles and sensory properties of strawberry cultivars grown both organically and conventionally.

    Science.gov (United States)

    Kårlund, Anna; Hanhineva, Kati; Lehtonen, Marko; Karjalainen, Reijo O; Sandell, Mari

    2015-01-28

    Strawberry (Fragaria × ananassa Duch.) contains many secondary metabolites potentially beneficial for human health, and several of these compounds contribute to strawberry sensory properties, as well. In this study, three strawberry cultivars grown both conventionally and organically were subjected to nontargeted metabolite profiling analysis with LC-qTOF-ESI-MS and to descriptive sensory evaluation by a trained panel. Combined metabolome and sensory data (PLS model) revealed that 79% variation in the metabolome explained 88% variation in the sensory profiles. Flavonoids and condensed and hydrolyzable tannins determined the orosensory properties, and fatty acids contributed to the odor attributes of strawberry. Overall, the results indicated that the chemical composition and sensory quality of strawberries grown in different cultivation systems vary mostly according to cultivar. Organic farming practices may enhance the accumulation of some plant metabolites in specific strawberry genotypes. Careful cultivar selection is a key factor for the improvement of nutritional quality and marketing value of organic strawberries.

  7. Melhoramento do cafeeiro: XXIX - Produtividade de cafeeiros de porte reduzido Coffee breeding: XXIX - Productivity of dwarf coffee cultivars

    Directory of Open Access Journals (Sweden)

    C.S. Novaes Antunes

    1964-01-01

    Full Text Available Os cultivares de porte pequeno mais promissores da coleção de cafeeiros da Seção de Genética foram plantados em um ensaio comparativo, instalado em 1954, em Campinas. Os dados de sete anos de colheitas dêsse ensaio revelaram que a progênie de prefixo C 1039-48 do cultivar 'São Bernardo' foi a de melhor produção, e a de prefixo C 1034-4 'San Ramon', a de produção mais reduzida. Outras observações foram efetuadas atinentes à altura das plantas, resistência à geada, tipos de sementes produzidas e tamanho das sementes do tipo chato, analisadas pela peneira média, a fim de melhor caracterizar êsses cultivares. Os resultados colhidos dão informações úteis para o trabalho de melhoramento, indicando plantas e progênies a serem utilizadas principalmente nas hibridações com outros cultivares selecionados.The increasing cost of labor is leading the breeders to select coffee strains with low height which give high yield per area and facilitate the harvesting operation. The present paper refers to a trial established to compare the yielding ability of twelve dwarf coffee progenies of 'Caturra Amarelo', 'Caturra Vermelho', 'San Ramon', 'San Ramon x Bourbon', 'San Ramon x Maragogipe' and 'São Bernardo'. After seven consecutive harvests the total weight of coffee cherries was analysed. It was found that only the progeny 'São Bernardo' - C 1039-48 had a significant higher yield than the tester 'Caturra Vermelho' - C 477-8. The two 'Caturra Amarelo' progenies were in the same group of C 1039-48. Small variation was noticed in connection with bean size and percentages of flat, peaberry and elephant beans. The progenies of cultivar 'São Bernardo' had the largest beans and the highest percentages of normal flat beans. Concerning frost resistance some variation was observed in the susceptibility of the progenies to low temperatures. Only the progeny C 1036-36 revealed to be resistant. The best plants selected in this trial will be used in

  8. Characterization of community structure of culturable endophytic fungi in sweet cherry composite trees and their growth-retarding effect against pathogens.

    Science.gov (United States)

    Haddadderafshi, Neda; Pósa, Tímea Borbála; Péter, Gábor; Gáspár, László; Ladányi, Márta; Hrotkó, Károly; Lukács, Noémi; Halász, Krisztián

    2016-09-01

    Endophytic fungi have the potential to protect their host plants in stress situations. Characterizing the ecology and complex interaction between these endophytes and their host plants is therefore of great practical importance, particularly in horticultural plants. Among horticultural plants, fruit trees form a special category because of their longevity and because they are composites of rootstock and scion, which often belong to different plant species. Here we present the first characterization of culturable endophytic fungal community of sweet cherry. Samples from the Hungarian cultivar 'Petrus' grafted on 11 different rootstocks were collected in autumn and in spring in a bearing orchard and the dependence of colonization rate and endophyte diversity on rootstock, organ and season was analysed. On the basis of their ITS sequences 26 fungal operational taxonomic units were identified at least down to the genus level. The dominant genus, comprising more than 50% of all isolates, was Alternaria, followed by different Fusarium and Epicoccum species. We observed some organ-specificity amongst endophytes, and organs showed more sizeable differences in colonization rates and endophyte diversity than rootstocks. Most dynamic endophyte populations, strongly influenced by environmental conditions and crop management, were observed in leaves. The potential of selected endophytes to confer protection against Monilinia laxa was also analysed and 7 isolates were found to inhibit the growth of this pathogen in vitro.

  9. Yield potential of pigeon pea cultivars

    Science.gov (United States)

    Yield potential of twelve vegetable pigeon pea (Cajanus cajun) cultivars was evaluated at two locations in eastern Kenya during 2012 and 2013 cropping years. Pigeon pea pod numbers, seeds per pod, seed mass, grain yield and shelling percentage were quantified in three replicated plots, arranged in a...

  10. Growth and development in Achimenes cultivars.

    NARCIS (Netherlands)

    Vlahos, J.C.

    1991-01-01

    Achimenes is a herbaceous perennial of the Gesneriaceae family. Its origin is Central and South America. It has been cultivated as a pot plant since the Victorian era. Extensive hybridization has produced many attractive cultivars which have been Introduced in commercial floriculture both In the Uni

  11. CULTIVAR RELEASE-CD 115: Soft Wheat Cultivar for colder regions

    Directory of Open Access Journals (Sweden)

    Volmir Sergio Marchioro

    2008-01-01

    Full Text Available The cultivar CD 115 was developed by the Cooperativa Central de Pesquisa Agrícola (COODETEC and isrecommended for cultivation in the states of Rio Grande do Sul, Santa Catarina and Paraná. The cultivar is tolerant todiseases and pre-harvest sprouting. The mean grain yield in four years of evaluation was 3,420 kg ha-1, exceeding thecontrols by 7%.

  12. CULTIVAR RELEASE - BRSMG União: common bean cultivar with jalo grain for the state of Minas Gerais

    Directory of Open Access Journals (Sweden)

    Magno Antonio Patto Ramalho

    2012-12-01

    Full Text Available The bean cultivar BRSMG União as a new option of a bean cultivar with jalo grains for the state of Minas Gerais. The cultivar BRSMG União had an average grain yield of 9.8% above the mean of the controls (Jalo EEP 558 and BRS Radiante and was resistant to powdery-mildew.

  13. Evaluation of genetic bases and diversity of Egyptian wheat cultivars released during the last 50 years using coefficient of parentage

    Directory of Open Access Journals (Sweden)

    Bhoja R. Basnet

    2011-04-01

    Full Text Available Discerning the genetic diversity of any crop species provides insight into the strength of an applied breeding program and directs future breeding strategies aimed at long-term genetic gain and minimized genetic vulnerability. The number and abundance of ancestral parents present in the pedigree of crop cultivars can provide an average estimation of the depth of the genetic base of the overall crop improvement program. The objectives of this study were to estimate (1 the genetic similarity among 33 Egyptian wheat (Triticum aestivum L. cultivars and different eras of release (1947-2004 and productivity groups based on COP values, and (2 the relative genetic contribution and abundance of ancestral parents from different geographical origins to the total gene pool of Egyptian wheat cultivars. Broad genetic diversity was observed among 33 Egyptian cultivars with average COP value of 0.11 and large numbers of ancestral parents (155 landraces traced to 31 countries. The genetic base ranged from very low in pre 1960’s cultivars such as ‘Giza 139’ (with only 3 landraces in the background to very high in modern cultivars such as ‘Gemmeiza-7’ (with 73 landraces in the background. ‘Hindi-62’, ‘Red Fife’, ‘Hard Red Calcutta’ and ‘Akagomughi’ were the major ancestors with 6, 5, 4, and 4% of total genetic contribution to the Egyptian wheat gene pool, respectively. Egypt, United States of America, Kenya and Ukraine were the major source countries with 16, 11, 9 and 7% of total genetic contribution to this gene pool, respectively. Though Marquis-Thatcher germplasm from North America has the greatest influence on overall Egyptian cultivars, Mexican-based sources of dwarfing and high yield, derived from ancestors such as ‘Akagomughi’ and ‘Daruma’ and exploited by the International Maize and Wheat Improvement Center (CIMMYT, were very prominent in Egyptian cultivars post 1970’s.

  14. Screening of Carnation Cultivars for Resistance to Meloidogyne incognita.

    Science.gov (United States)

    Cho, M R; Kim, J Y; Song, C; Ko, J Y; Na, S Y; Yiem, M S

    1996-12-01

    A total of 33 carnation cultivars cultured in Korea were screened for resistance to the southern root-knot nematode, Meloidogyne incognita. Carnations were tested by either inoculating with 5,000 eggs or by transplanting into a mixture of bedding medium and soil infested with an average of 435 second-stage juveniles/300 cm(3) soil. Cultivars, Desio, Castelaro, Kappa, Rara, Izu Pink, Target, and Antalia were highly resistant to M. incognita. Twelve cultivars were moderately resistant, and the remaining 14 cultivars were susceptible. These results were similar to those obtained when the cultivars were subjected to field populations of the condition on a carnation farm.

  15. Screening of sunflower cultivars for metal phytoextraction in a contaminated field prior to mutagenesis.

    Science.gov (United States)

    Nehnevajova, Erika; Herzig, Rolf; Federer, Guido; Erismann, Karl-Hans; Schwitzguébel, Jean-Paul

    2005-01-01

    Sunflower can be used for the remediation of metal-contaminated soils. Its high biomass production makes this plant species interestingfor phytoextraction and using sunflower oil for a technical purpose may improve the economic balance of phytoremediation. The aim of the present field study was to screen 15 commercial cultivars of Helianthus annuus L. grown on metal-contaminated soil, to find out the variety with the highest metal extraction, which can be further improved by mutation or in vitro breeding procedures. Two different fertilizers (ammonium sulphate and ammonium nitrate) were also used to enhance the bioavailability of metals in soil Highly significant differences were observed within tested varieties for metal accumulation and extraction efficiency. Furthermore, ammonium nitrate increased cadmium extraction, whereas ammonium sulphate enhanced zinc and lead uptake in most tested cultivars. In this field-based sunflower screening, we found enhanced cumulative Cd, Zn, and Pb extraction efficiency by a factor 4.4 for Salut cultivar. We therefore emphasize that prior to any classical breeding or genetic engineering enhancing metal uptake potential, a careful screening of various genotypes should be done to select the cultivar with the naturally highest metal uptake and to start the genetic improvement with the best available plant material.

  16. The effect of rootstocks on the growth and yielding of sour cherry cv. 'Łutówka'

    Directory of Open Access Journals (Sweden)

    Stanisław Wociór

    2012-12-01

    Full Text Available The strength of growth of 'Łutówka' trees was related to the soil quality and the rootstock. Measurements of the tree trunks and the crown size showed that on fertile soil the trees grafted on Mahaleb cherry grew worse, forming by 12% thinner trunks and by over 20% (significantly smaller crowns as compared to Mazzard cherry trees. On poor sandy-loamy soil the crowns of trees grafted on Mahaleb were significantly, more than 40% bigger than those on Mazzard cherry. No significant differences in the yielding and productivity of trees grafted on Mahaleb and Mazzard cherry trees were found on grey brown podzolic soil. The experiment conducted on sandy-loamy soil pointed to significant differences in the yielding and productivity between 'Łutówka' trees grafted on the studied rootstocks. The trees grafted on Mahaleb cherry in both studied years were characterized by significantly greater productivity than on the other rootstocks. Significantly higher yields were gathered from the trees on Mahaleb cherry than on vegetative rootstocks, and by 70% higher than on Mazzard cherry. P-HL A rootstock is of little use in the planting of 'Łutówka', which grown poorly on light soils. Besides poor growth and yielding, in longer dry periods the studies found the appearance of chlorosis of magnesium on older leaves, and even wilting of the leaves.

  17. 圣女果分段式变温变湿热风干燥特性%Dried characteristics of cherry tomatoes using temperature and humidity by stages changed hot-air drying method

    Institute of Scientific and Technical Information of China (English)

    王庆惠; 李忠新; 杨劲松; 谢龙; 张世湘; 高振江

    2014-01-01

    Cherry tomato decays easily. Generally, it can be preserved only for 5-7days under normal pressure and temperature. The dehydration of cherry tomatoes into dried or preserved fruits can prolong their shelf life and enrich the market of the fruit. Currently, cherry tomatoes is dehydrated through natural drying. The advantage of this method is simple, and low cost. But the drying time is long and is affected by the climate and sanitary conditions. The product quality is difficult to control. In order to shorten the drying time and prevent mildew, cherry tomatoes are often soaked or sprayed with the alkaline solution. These chemicals are bad for our health. Multi-stage temperature-and-humidity drying is conducted through the material drying characteristics. This method can speed up the drying rate, improve drying quality, and reduce energy consumption. It has been successfully applied for apricot,lyceum barbarum, grapes and other materials. In this study, internal recycling hot-air drying technology was employed for the drying of cherry tomatoes. The effects of drying temperatures, humidity, drying stages and slicing forms on the drying characteristics and the appearance quality of the cherry tomatoes were investigated. Each experiment was composed of four drying stages. The drying temperatures were fixed, the humidity of the environment was changed in processⅠ,Ⅱ andⅢ. The drying temperature was low, the humidity was high at the beginning of processⅣ. Gradually, the temperature was raised, the humidity was reduced. At the end of the process, the drying temperature was higher, the humidity was lower compared with their values at the beginning. The experimental results indicated that pre-heated and falling rate periods exist during the drying of cherry tomato. Previous studies only shown falling rate period. The increase in temperature and humidity has bad effects on the nutrition, color and the appearance quality of cherry tomato. Even though cherry tomato can

  18. "Clever v. Cherry Hill Township Board of Education": Recognition of Religious Diversity in Public Schools.

    Science.gov (United States)

    Mawdsley, Ralph D.

    1994-01-01

    A New Jersey school board enacted a policy to broaden students' understanding of their multicultural community's religious heritage. The federal district court upheld the Cherry Hill Township's approach to recognizing religious diversity in public schools. Reviews the policy and the court decision, and explores implications for public schools. (90…

  19. There Is Space to Play! Mexican American Children of Immigrants Learning With(in) Cherry Orchards

    Science.gov (United States)

    Morales, María Isabel

    2016-01-01

    While some children spend their summers in camps or other recreational activities, many children of immigrants in Washington state spend them picking cherries and learning with(in) orchards. Children's experiences consist of multiple narratives demonstrating that children's lives are complicated, yet full of possibilities for teaching and…

  20. Giant Paperclip Necklaces, Soup-Can Rings and Cherry-Pie Hats

    Science.gov (United States)

    Winters, Laurel A.

    2011-01-01

    In this article, the author describes an art project inspired by the wearable sculpture art created by artist Marjorie Schick. Students used wallpaper paste and newspapers to create papier-mache for a mountain hat, a cherry-pie mask/hat, a "dress" shoe and a Cubistic mask. Cardboard was used in many of these things, in addition to being used as…

  1. Before harvest survival of codling moth (Lepidoptera: Tortricidae) in artificially infested sweet cherries

    Science.gov (United States)

    Prior to the 2009 season, sweet cherries, Prunus avium (L.) L., from North America were required to be fumigated with methyl bromide before being exported to Japan to eliminate possible infestation by codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae). However, based on recent biological...

  2. Nutritional Value and Volatile Compounds of Black Cherry (Prunus serotina Seeds

    Directory of Open Access Journals (Sweden)

    Leticia García-Aguilar

    2015-02-01

    Full Text Available Prunus serotina (black cherry, commonly known in Mexico as capulín, is used in Mexican traditional medicine for the treatment of cardiovascular, respiratory, and gastrointestinal diseases. Particularly, P. serotina seeds, consumed in Mexico as snacks, are used for treating cough. In the present study, nutritional and volatile analyses of black cherry seeds were carried out to determine their nutraceutical potential. Proximate analysis indicated that P. serotina raw and toasted seeds contain mostly fat, followed by protein, fiber, carbohydrates, and ash. The potassium content in black cherry raw and toasted seeds is high, and their protein digestibility-corrected amino acid scores suggest that they might represent a complementary source of proteins. Solid phase microextraction and gas chromatography/flame ionization detection/mass spectrometry analysis allowed identification of 59 and 99 volatile compounds in the raw and toasted seeds, respectively. The major volatile compounds identified in raw and toasted seeds were 2,3-butanediol and benzaldehyde, which contribute to the flavor and odor of the toasted seeds. Moreover, it has been previously demonstrated that benzaldehyde possesses a significant vasodilator effect, therefore, the presence of this compound along with oleic, linoleic, and α-eleostearic fatty acids indicate that black cherry seeds consumption might have beneficial effects on the cardiovascular system.

  3. Influence of tart cherry juice on indices of recovery following marathon running.

    Science.gov (United States)

    Howatson, G; McHugh, M P; Hill, J A; Brouner, J; Jewell, A P; van Someren, K A; Shave, R E; Howatson, S A

    2010-12-01

    This investigation determined the efficacy of a tart cherry juice in aiding recovery and reducing muscle damage, inflammation and oxidative stress. Twenty recreational Marathon runners assigned to either consumed cherry juice or placebo for 5 days before, the day of and for 48 h following a Marathon run. Markers of muscle damage (creatine kinase, lactate dehydrogenase, muscle soreness and isometric strength), inflammation [interleukin-6 (IL-6), C-reactive protein (CRP) and uric acid], total antioxidant status (TAS) and oxidative stress [thiobarbituric acid reactive species (TBARS) and protein carbonyls] were examined before and following the race. Isometric strength recovered significantly faster (P=0.024) in the cherry juice group. No other damage indices were significantly different. Inflammation was reduced in the cherry juice group (IL-6, Pcherry juice than the placebo group for all post-supplementation measures (Pcherry juice than the placebo at 48 h (Pcherry juice appears to provide a viable means to aid recovery following strenuous exercise by increasing total antioxidative capacity, reducing inflammation, lipid peroxidation and so aiding in the recovery of muscle function.

  4. Effect of gamma-irradiation on the survival of Listeria monocytogenes and allergenicity of cherry tomatoes

    Energy Technology Data Exchange (ETDEWEB)

    Todoriki, Setsuko [National Food Research Institute, Tsukuba, Ibaraki 305-8642 (Japan)], E-mail: setsuko@affrc.go.jp; Bari, Latiful; Kitta, Kazumi; Ohba, Mika; Ito, Yasuhiro; Tsujimoto, Yuka [National Food Research Institute, Tsukuba, Ibaraki 305-8642 (Japan); Kanamori, Norihito [Japan International Research Center for Agricultural Science, Tsukuba, Ibaraki 305-8686 (Japan); Yano, Erika; Moriyama, Tatsuya; Kawamura, Yukio [School of Agriculture, Kinki University, Nara-city, Nara 631-8505 (Japan); Kawamoto, Shinichi [National Food Research Institute, Tsukuba, Ibaraki 305-8642 (Japan)

    2009-07-15

    The presence of Listeria monocytogenes in fresh produce is a growing concern because of the possibility of food-borne illness. Ionizing radiation is an effective non-thermal means of eliminating pathogenic bacteria in fresh produce; however, the effect of ionizing irradiation on the allergenic properties of the host commodities remains unknown. This study aimed (i) to determine the effective dose of gamma-irradiation in eliminating L. monocytogenes on whole cherry tomatoes and (ii) to evaluate the effect of gamma-irradiation on the allergenic properties of tomato proteins. Cherry tomatoes that were inoculated with a mixture of five L. monocytogenes strains were treated with gamma-rays from a {sup 60}Co source. A 1.25 kGy dose of gamma-irradiation was found to be sufficient to eliminate L. monocytogenes on whole cherry tomatoes. The immunoblot profile of serum samples obtained from two patients with tomato allergy revealed that gamma-irradiation did not affect the allergenicity of tomato proteins for up to 7 days after irradiation when the tomatoes were stored at 20 deg. C. Additionally, the m-RNA levels of {beta}-fructofuranosidase, polygalacturonase, pectin esterase, and superoxide dismutase, the main allergenic proteins in tomato, were not affected by the applied irradiation dose. Thus, this study demonstrated that a 1.25 kGy dose of gamma-irradiation effectively eliminates L. monocytogenes on cherry tomatoes without affecting the expression of allergenic proteins in the fruits.

  5. Tart cherry extracts reduce inflammatory and oxidative stress signaling in microglial cells

    Science.gov (United States)

    Tart cherries contain an array of polyphenols that can decrease inflammation and oxidative stress (OS), which contribute to cognitive declines seen in aging populations. Previous studies have shown that polyphenols from dark-colored fruits can reduce stress-mediated signaling in BV-2 mouse microglia...

  6. Characterization of R genes involved in resistance to Cherry leaf roll virus in paradox hybrids

    Science.gov (United States)

    A single dominant ‘R’ gene (clrvR), in black walnuts (Juglans hindsii) or ‘paradox’ hybrids (J. hindsii x J. regia) confers resistance to Cherry leaf roll virus (CLRV), the causal agent of blackline disease. The identification and cloning of the ‘R’ gene is expected to aid the walnut breeding progra...

  7. Cherry Picking Robot Vision Recognition System Based on OpenCV

    Directory of Open Access Journals (Sweden)

    Zhang Qi Rong

    2016-01-01

    Full Text Available Through OpenCV function, the cherry in a natural environment image after image preprocessing, color recognition, threshold segmentation, morphological filtering, edge detection, circle Hough transform, you can draw the cherry’s center and circular contour, to carry out the purpose of the machine picking. The system is simple and effective.

  8. Reduction of Chilling Injury and Ultrastructural Damage in Cherry Tomato Fruits After Hot Water Treatment

    Institute of Scientific and Technical Information of China (English)

    YANG Jing; FU Mao-run; ZHAO Yu-ying; MAO Lin-chun

    2009-01-01

    The effects of hot water treatment in alleviating chilling injury and reducing ultrastructural damage of mature-green cherry tomatoes (Lycopersicun esculentum cv. cerasiform Alef) were investigated. Mature-green cherry tomato fruits were treated in water at 40℃ or 45℃ for 5 rain or 15 rain, and then stored at 5℃ for 19 days followed by ripening at 20℃. Water treatment at 40℃ for 15 rain increased tolerance of cherry tomato fruits to chilling stress, indicating as low outbreak of skin lesion, high color a* value, and low electrolyte leakage. Treated fi'uits showed typical climacteric respiration and developed normal red color with chlorophyll degradation and lyeopene accumulation during ripening, while fruits without treatment failed to develop red color and suffered skin lesion. After 19 days of chilling, heated fruits showed the conversion of chloroplast to ehromoplast with the disappearance of thylakoids. Mitochondria and other cell organelles were not adversely affected in treated fruits. However, ultrastruetures in periearp cells in control fruits severely damaged with extensive disorganization of cytoplasm, swelled chloroplasts, distorted and unstacked thylakoids. Chloroplast was the first and most severely impacted organelle by chilling stress. Hot water treatment (40℃ for 15 min) before storage alleviated chilling injury in cherry tomato fruits. The results suggest that chilling injury is related with the damage of cell structure under chilling stress.

  9. Relation between polyphenols content and skin colour in sour cherry fruits

    Directory of Open Access Journals (Sweden)

    Viljevac Marija

    2012-01-01

    Full Text Available Fruit skin colour plays a major role in quality assessment of food, significantly determining consumer`s choice. Colour of sour cherries depends on anthocyanins which are phenolic compounds (flavonoids present in high amounts in fruits. The aim of this study was to determine a possible relation between polyphenols (total phenolics and anthocyanins and colour parameters of fruit skin of sour cherries. The plant material used in this study was twenty two sour cherry genotypes from an orchard of Agricultural Institute Osijek. Total phenolics and anthocyanins contents as well as colour parameters (L*, a*, b*, h and C of fruit skin were determined. Variability between sour cherry genotypes in total phenolics and anthocyanins as well as in colour parameters was revealed. Total polyphenols content varied from 462.7 to 1049.0 mg GAE/100 g of fresh weight, while total anthocyanins ranged from 160.1 to 495.6 mg CGE/100 g of fresh weight. A significant positive correlation was found between polyphenols and anthocyanins content. Total phenolics content had a significant negative correlation with colour parameters b* and h, while anthocyanins content negatively correlated with colour parameters L*, b* and h. According to the obtained results, genotypes Maraska, Heimanns Konservenweichsel and Rexelle are the richest genotypes in polyphenols and anthocyanins content.

  10. Effect of gamma-irradiation on the survival of Listeria monocytogenes and allergenicity of cherry tomatoes

    Science.gov (United States)

    Todoriki, Setsuko; Bari, Latiful; Kitta, Kazumi; Ohba, Mika; Ito, Yasuhiro; Tsujimoto, Yuka; Kanamori, Norihito; Yano, Erika; Moriyama, Tatsuya; Kawamura, Yukio; Kawamoto, Shinichi

    2009-07-01

    The presence of Listeria monocytogenes in fresh produce is a growing concern because of the possibility of food-borne illness. Ionizing radiation is an effective non-thermal means of eliminating pathogenic bacteria in fresh produce; however, the effect of ionizing irradiation on the allergenic properties of the host commodities remains unknown. This study aimed (i) to determine the effective dose of gamma-irradiation in eliminating L. monocytogenes on whole cherry tomatoes and (ii) to evaluate the effect of gamma-irradiation on the allergenic properties of tomato proteins. Cherry tomatoes that were inoculated with a mixture of five L. monocytogenes strains were treated with gamma-rays from a 60Co source. A 1.25 kGy dose of gamma-irradiation was found to be sufficient to eliminate L. monocytogenes on whole cherry tomatoes. The immunoblot profile of serum samples obtained from two patients with tomato allergy revealed that gamma-irradiation did not affect the allergenicity of tomato proteins for up to 7 days after irradiation when the tomatoes were stored at 20 °C. Additionally, the m-RNA levels of β-fructofuranosidase, polygalacturonase, pectin esterase, and superoxide dismutase, the main allergenic proteins in tomato, were not affected by the applied irradiation dose. Thus, this study demonstrated that a 1.25 kGy dose of gamma-irradiation effectively eliminates L. monocytogenes on cherry tomatoes without affecting the expression of allergenic proteins in the fruits.

  11. Hemangioma rubi no couro cabeludo Cherry hemangioma in the scalp

    Directory of Open Access Journals (Sweden)

    José Marcos Pereira

    2004-02-01

    Full Text Available O hemangioma rubi (HR é dermatose de origem vascular extremamente freqüente, acometendo mais de 75% da população acima de 70 anos de idade. Em geral compõe-se de lesões múltiplas,localizadas predominantemente no alto do tronco e braços. Clinicamente é caracterizado por lesões que variam desde máculas puntiformes até lesões papulosas com cinco milímetros de diâmetro. As mais novas são vermelhas, em tons vivos, e as mais antigas podem ser azuladas. O HR é de etiologia desconhecida. Histologicamente chama atenção uma neoformação de vasos capilares, que se tornam dilatados e com fenestrações em suas paredes. A membrana basal está muito espessada e existe abundante estroma de colágeno entre os vasos. O presente trabalho demonstra a alta incidência do HR no couro cabeludo Em amostra de 171 pacientes, sendo 85 homens e 86 mulheres, o autor observou que 123 deles (72% tinham HR no couro cabeludo, localização em que o HR nunca foi descrito na literatura.Cherry hemangioma (CH is an extremely frequent dermatosis with vascular origin involving more than 75% of the population over 70 years of age. Normally they are multiplex spots and focus predominantly on the upper trunk and arms. Clinically they are characterized by pinpoint maculae and papules with up to 5 millimeters in diameter. The most recent lesions can be a strong red color while the older ones are bluish. The etiology of CH is still unknown. From the histologic standpoint, the neoformation of the capillary tube draws attention: they are very dilated and with fenestration along the wall. The basement membranes are thickened and there is abundant collagen stroma between the veins. This work demonstrates the high frequency of CH in the scalp. In a sample of 171 patients (85 men and 86 women the author noted that 123 (72% had CH in the scalp. However, according to the literature, CH in the scalp has never been described.

  12. Design of ultrasonic probe and evaluation of ultrasonic waves on E.coli in Sour Cherry Juice

    Directory of Open Access Journals (Sweden)

    B Hosseinzadeh Samani

    2015-09-01

    Full Text Available Introduction: The common method used for juice pasteurization is the thermal method since thermal methods contribute highly to inactivating microbes. However, applying high temperatures would lead to inefficient effects on nutrition and food value. Such effects may include vitamin loss, nutritional flavor loss, non-enzyme browning, and protein reshaping (Kuldiloke, 2002. In order to decrease the adverse effects of the thermal pasteurization method, other methods capable of inactivation of microorganisms can be applied. In doing so, non-thermal methods including pasteurization using high hydrostatic pressure processing (HPP, electrical fields, and ultrasound waves are of interest (Chen and Tseng, 1996. The reason for diminishing microbial count in the presence of ultrasonic waves could be due to the burst of very tiny bubbles developed by ultrasounds which expand quickly and burst in a short time. Due to this burst, special temperature and pressure conditions are developed which could initiate or intensify several physical and/or chemical reactions. The aim of this study is to evaluate the non-thermal ultrasonic method and its effective factors on the E.coli bacteria of sour cherry. Materials and methods: In order to supply uniform ultrasonic waves, a 1000 W electric generator (Model MPI, Switzerland working at 20±1 kHz frequency was used. The aim of this study is to evaluate the non-thermal ultrasonic method and its effective factors on the E.coli bacteria of sour cherry. For this purpose, a certain amount of sour cherry fruit was purchased from local markets. First, the fruits were washed, cleaned and cored. The prepared fruits were then dewatered using an electric juicer. In order to separate pulp suspensions and tissue components, the extracted juice was poured into a centrifuge with the speed of 6000 rpm for 20 min. For complete separation of the remaining suspended particles, the transparent portion of the extract was passed through a

  13. Early-screening for resistance to Phytophthora sp.p. in wild cherry clones (Prunus avium L.

    Directory of Open Access Journals (Sweden)

    Guerri S

    2004-01-01

    Full Text Available A new method for early selection of wild cherry clones for resistance to Phytophthora sp. is presented. Four Phytophthora species (P. cinnamomi, P. citrophthora, P. megasperma, P. alni were tested in vitro on four micropropagated cherry (Prunus avium clones, obtaining reliable and reproducible results. Variability in clones susceptibilities and in parasite virulence has been evidenced. Phytophthora citrophthora is confirmed to be a dangerous parasite, due to its capability to produce symptoms rapidly and its fitness to Mediterranean environments. On the contrary P. alni does not seem a dangerous parasite for wild cherry.

  14. Nucleotide diversity and phylogenetic relationships among Gladiolus cultivars and related taxa of family Iridaceae

    Indian Academy of Sciences (India)

    NIRAJ SINGH; BALESHWAR MEENA; ASHISH KUMAR PAL; ROOP KUMAR ROY; SRI KRISHNA TEWARI; SUSHMA TAMTA; TIKAM SINGH RANA

    2017-03-01

    The plastid genome regions of two intergenic spacers, psbA–trnH and trnL–trnF, were sequenced to study the nucleotide diversity and phylogenetic relationships among Gladiolus cultivars. Nucleotide diversity of psbA–trnH region was higher than trnL–trnF region of chloroplast. We employed Bayesian, maximum parsimony (MP) and neighbour-joining (NJ) approaches for phylogenetic analysis of Gladiolus and related taxa using combined datasets from chloroplast genome. The psbA–trnH and trnL–trnF intergenic spacers of Gladiolus and related taxa-like Babiana, Chasmanthe, Crocus, Iris, Moraea, Sisyrinchium,Sparaxis and two out group species (Hymenocallis littoralis and Asphodeline lutea) were used in the present investigation. Results showed that subfamily Iridoideae have sister lineage with subfamily Ixioideae and Crocoideae. H. littoralis and A. lutea were separately attached at the base of tree as the diverging Iridaceae relative’s lineage. Present study revealed that psbA–trnH region are useful in addressing questions of phylogenetic relationships among the Gladiolus cultivars, as these intergenic spacers are more variable and have more phylogenetically informative sites than the trnL–trnF spacer, and therefore,are suitable for phylogenetic comparison on a lower taxonomic level. Gladiolus cultivars are extensively used as an ornamental crop and showed high potential in floriculture trade. Gladiolus cultivation still needs to generate new cultivars with stable phenotypes. Moreover, one of the most popular methods for generating new cultivars is hybridization. Hence, information on phylogenetic relationships among cultivars could be useful for hybridization programmes for further improvement of the crop.

  15. Seed Priming to Overcome Salinity Stress in Persian Cultivars of Alfalfa (Medicago sativa L.

    Directory of Open Access Journals (Sweden)

    Ali SEPEHRI

    2015-03-01

    Full Text Available In order to investigate the effect of hydro-priming on seed germination with distilled water on germination of five Alfalfa cultivars under salinity stress, an experiment was conducted as a factorial experiment based on a completely randomized design with three replications. Seven levels of hydro-priming and salinity of NaCl including prime and non-salinity, prime and 50 mM salinity, prime and 100 mM salinity, prime and 150 mM salinity, prime and 200 mM salinity, prime and 250 mM salinity and without prime and salinity and five alfalfa varieties, including ‘Hamedani’, ‘Isfahani’, ‘Bami’, ‘Yazdi’ and ‘Ghareh Yonjeh’ were used. The results showed that the main effect of prime, salinity and cultivars and their interaction in all studied traits were significantly affected at the 5% probability level. Priming treatments in non-salinity of all cultivars were the highest. In all cultivars, final germination percentage, length and weight of radicle, plumule and seedling, germination rate and time, relative radicle elongation, vigor index and stress index, were significantly improved in response to priming in salinity levels of 50-200 mM, compared to control. Radicle produced higher length and weight than the plumule in hydro- priming and salinity treatments. ‘Hamedani’ cultivar in most of studied characteristics had a better response than others. The lowest response to salinity stress and priming was observed in ‘Yazdi’ cultivar.

  16. Performance of a Horizontal Double Cylinder Type of Fresh Coffee Cherries Pulping Machine

    Directory of Open Access Journals (Sweden)

    Sukrisno Widyotomo

    2009-05-01

    Full Text Available Pulping is one important step in wet coffee processing method. Usually, pulping process uses a machine which constructed using wood or metal materials. A horizontal single cylinder type coffee pulping machine is the most popular machine in coffee processor and market. One of the weakness of a horizontal single cylinder type coffee pulping machine is high of broken beans. Broken beans is one of major aspect in defect system that result in low quality. Indonesian Coffee and Cocoa Research Institute has designed and tested a horizontal double cylinder type coffee pulping machine. Material tested is Robusta cherry, mature, 60—65% (wet basis moisture content, which size compostition of coffee cherries was 50.8% more than 15 mm diameter, 32% more than 10 mm diameter, and 16.6% to get through 10 mm hole diameter; 690—695 kg/m3 bulk density, and clean from methal and foreign materials. The result showed that this machine has 420 kg/h optimal capacity in operational conditions, 1400 rpm rotor rotation speed for unsorted coffee cherries with composition 53.08% whole parchment coffee, 16.92% broken beans, and 30% beans in the wet skin. For small size coffee cherries, 603 kg/h optimal capacity in operational conditions, 1600 rpm rotor rotation speed with composition 51.30% whole parchment coffee, 12.59% broken beans, and 36.1% beans in the wet skin. Finally, for medium size coffee cherries, 564 kg/h optimal capacity in operational conditions, 1800 rpm rotor rotation speed with composition 48.64% whole parchment coffee, 18.5% broken beans, and 32.86% beans in the wet skin.Key words : coffee, pulp, pulper, cylinder, quality.

  17. Mapping of Candidate Genes Involved in Bud Dormancy and Flowering Time in Sweet Cherry (Prunus avium.

    Directory of Open Access Journals (Sweden)

    Sophie Castède

    Full Text Available The timing of flowering in perennial plants is crucial for their survival in temperate climates and is regulated by the duration of bud dormancy. Bud dormancy release and bud break depend on the perception of cumulative chilling during endodormancy and heat during the bud development. The objectives of this work were to identify candidate genes involved in dormancy and flowering processes in sweet cherry, their mapping in two mapping progenies 'Regina' × 'Garnet' and 'Regina' × 'Lapins', and to select those candidate genes which co-localized with quantitative trait loci (QTLs associated with temperature requirements for bud dormancy release and flowering. Based on available data on flowering processes in various species, a list of 79 candidate genes was established. The peach and sweet cherry orthologs were identified and primers were designed to amplify sweet cherry candidate gene fragments. Based on the amplified sequences of the three parents of the mapping progenies, SNPs segregations in the progenies were identified. Thirty five candidate genes were genetically mapped in at least one of the two progenies and all were in silico mapped. Co-localization between candidate genes and QTLs associated with temperature requirements and flowering date were identified for the first time in sweet cherry. The allelic composition of the candidate genes located in the major QTL for heat requirements and flowering date located on linkage group 4 have a significant effect on these two traits indicating their potential use for breeding programs in sweet cherry to select new varieties adapted to putative future climatic conditions.

  18. THE VARIABILITY OF THE MORPHOLOGICAL TRAITS OF DIPLOID RED CLOVER CULTIVARS STUDIED IN CLUJ-NAPOCA ENVIRONMENTAL CONDITIONS

    Directory of Open Access Journals (Sweden)

    Leon jr. MUNTEAN

    2005-08-01

    Full Text Available The valuable characteristics of red clover (productivity, resistance to biotic and non-biotic factors, as well as quality depend on a series of morphological and physiological traits, which, in turn, are influenced by genetic and environmental factors. The purpose of our research, carried out at Cluj-Napoca, was to study the variability of the morphological and physiological traits of 45 diploid red clover cultivars, as well as their impact on productivity and the quality of the forage. In the breeding programmes carry out at Cluj-Napoca, in order to improve the morphological traits responsible for green matter yields, recommend that cultivars from Central and Western Europe should be used (subvar. intermedium. Were noticed, within this subvariety, the cultivars originating from Switzerland (Renova, Ruttinova, because of their increased earliness and good after-cut regeneration capacity. The late North-European cultivars (var. serotinum are valuable for increasing the leaf percentage and cold resistance. It was noticed that cold resistance is a characteristic of this variety and that there is no obvious variability among the cultivars from that area. The cultivars with very good resistance to mildew powder were those from southern Europe (subvar. praecox; this characteristic is one of the most important goals of the breeding programmes in the origin area, given the specific climatic conditions (increased soil and air drought.

  19. Identification of rice cultivars resistant to Lissorhoptrus oryzophilus (Coleoptera: Curculionidae), and their use in an integrated management program.

    Science.gov (United States)

    Stout, M J; Rice, W C; Linscombe, S D; Bollich, P K

    2001-08-01

    The rice water weevil, Lissorhoptrus oryzophilus Kuschel, is the most destructive insect pest of rice in the United States and is a particularly severe pest in Louisiana. The current management program for this insect in Louisiana relies heavily on insecticides, most notably the seed treatment fipronil (Icon). Diversification of the management program by incorporation of alternative strategies is needed to improve the effectiveness and long-term stability of the program. In the three experiments reported here, three components of a diversified management program for the rice water weevil in Louisiana--host plant resistance, treatment of seeds with Icon, aid the cultural practice of delayed flooding--were investigated. Comparison of the densities of weevil larvae on the roots of several commercial cultivars indicated that the long-grain cultivar 'Jefferson' was more resistant to infestation by the rice water weevil than the other cultivars. The medium-grain cultivars 'Bengal', 'Earl' and 'Mars' and the long-grain variety 'Cocodrie' were the most susceptible to infestation. Comparison of yield data from untreated plots and plots treated with Icon indicated that the long-grain cultivars Cocodrie, Lemont, and Jefferson were more tolerant of weevil injury than the other cultivars. A 2-wk delay in flooding was associated with yield benefits in plots not treated with Icon. Treatment of seeds with Icon controlled weevils in all three screening experiments. The implications of these results for the development of an integrated management program for the rice water weevil are discussed.

  20. Textural properties of mango cultivars during ripening.

    Science.gov (United States)

    Jha, Shyam Narayan; Jaiswal, Pranita; Narsaiah, Kairam; Kaur, Poonam Preet; Singh, Ashish Kumar; Kumar, Ramesh

    2013-12-01

    Firmness and toughness of fruit, peel and pulp of seven different mango cultivars were studied over a ripening period of ten days to investigate the effects of harvesting stages (early, mid and late) on fruit quality. Parameters were measured at equatorial region of fruits using TA-Hdi Texture Analyzer. The textural characteristics showed a rapid decline in their behaviour until mangoes got ripened and thereafter, the decline became almost constant indicating the completion of ripening. However, the rate of decline in textural properties was found to be cultivar specific. In general, the changes in textural attributes were found to be significantly influenced by ripening period and stage of harvesting, but firmness attributes (peel, fruit and pulp) of early harvested mangoes did not differ significantly from mid harvested mangoes, while peel, fruit and pulp firmness of late harvested mangoes were found to be significantly lower than early and mid harvested mangoes.

  1. RB975952 – Early maturing sugarcane cultivar

    Directory of Open Access Journals (Sweden)

    Monalisa Sampaio Carneiro

    2015-08-01

    Full Text Available RB975952 is an early maturing sugarcane cultivar released for the South-Central region of Brazil. It should be harvested between April and May, and it is recommended for planting in environments with medium to high production potential. RB975952 has high resistance levels to the main diseases of the crop, it also has a good shoot development after mechanical harvesting, and high sucrose yields.

  2. Relative competitiveness of soybean cultivars with barnyardgrass

    Directory of Open Access Journals (Sweden)

    Marlon Ouriques Bastiani

    Full Text Available ABSTRACT The aim of this work was to evaluate the competitiveness between soybean cultivars and barnyardgrass, based on morphological and physiological characteristics of species. The experiments were conducted in completely randomized experimental design, with 4 replications. In the first study, for both soybean and barnyardgrass, it was determined the population of plants in which shoot dry matter became constant and independent of the population (16 plants∙pot−1 or 400 plants∙m−2. In the second study, 2 experiments were conducted to evaluate the competitiveness of BMX Apolo RR and BMX Potência RR soybean cultivars with barnyardgrass plants, both carried out in replacement series under different proportions of plants∙pot−1 (100:0; 75:25; 50:50; 25:75 and 0:100 between the crop and the weed. The analysis of the species competitiveness was determined through diagrams applied to replacement series experiments and use of relative competitiveness indexes. At 44 days after the emergence of species, the physiological and morphological parameters of the crop and the weed were evaluated. The BMX Apolo RR and BMX Potência RR soybean cultivars show similar competitiveness when competing with the barnyardgrass; therefore, the ability of one species to interfere on another is equivalent. For plant height, barnyardgrass displays higher competitiveness compared to BMX Apolo RR, with early cycle and short height. The intraspecific competition is more important to barnyardgrass than interspecific competition with soybean cultivars, resulting in negative effects on the morphological and physiological characteristics of species.

  3. Evaluation of garlic cultivars for polyphenolic content and antioxidant properties.

    Science.gov (United States)

    Chen, Shuxia; Shen, Xiaoqing; Cheng, Siqiong; Li, Panpan; Du, Junna; Chang, Yanxia; Meng, Huanwen

    2013-01-01

    Two phenolic compound parameters (total phenolic and flavonoid contents) and 5 antioxidant parameters (DPPH [2, 2-diphenyl-1-picrylhydrazyl] radical scavenging activity, HRSC (hydroxyl radical scavenging capacity), FRAP (ferric ion reducing antioxidant power), CUPRAC (cupric ion reducing antioxidant capacity), and MCA (metal chelating activity) were measured in bulbs and bolts of 43 garlic cultivars. The bulbs of cultivar '74-x' had the highest phenolic content (total phenolic, flavonoids) and the strongest antioxidant capacity (DPPH, FRAP, and CUPRAC), followed by bulbs of cultivar 'Hanzhong purple'; the bulbs of cultivar 'Gailiang' had the lowest phenolic content and antioxidant capacity (FRAP, CUPRAC, MCA). The bolts of 'Hanzhong purple' also had higher phenolic content. Principal components analysis (PCA) separated the cultivars into 3 groups according to phenolic and flavonoid contents and strength of antioxidant activity. The first group had higher HRSC, FRAP, and flavonoid content; the second group had higher total phenolic content and MCA; some cultivars in the third group had higher HRSC and FRAP. All 8 test garlic bulb extracts successfully prevented Human Vascular Endothelial Cell death and significantly prevented reactive-oxygen species (ROS) formation in oxidative stress model, in which cultivar '74-x' had highest protection capability, following by cultivar 'Hanzhong purple', and the bulbs of cultivar 'No. 105 from Korea' had the lower protection capability against cell death and ROS formation. The protection capability in vivo of these garlic cultivars was consistent with their phenolic content and antioxidant capacity.

  4. 乳清蛋白可食性膜对圣女果贮藏品质的影响%Effect of Whey Protein Edible Film on Storage Quality of Cherry Tomatoes

    Institute of Scientific and Technical Information of China (English)

    阳晖; 雍凯

    2011-01-01

    [目的]研究乳清蛋白可食性膜对圣女果贮藏品质的影响.[方法] 以乳清废液为主要材料制备乳清蛋白膜,并将其用于圣女果的保鲜. [结果] 制膜的最佳配方为固液比1∶8,甘油添加量0.2%,干燥温度80 ℃.经乳清蛋白涂膜处理后的圣女果,在20 ℃下的贮藏品质明显提高.[结论]乳清蛋白可食性膜在一定程度上减缓了圣女果腐烂、失重、变软,具有一定的保鲜作用;而添加了山梨酸钾的乳清蛋白膜对圣女果的保鲜效果明显优于未添加山梨酸钾的乳清蛋白膜.%[ Objective ] The research aimed to study the effect of whey protein edibal film on the storage quality of cherry tomatoes. [ Method]Whey which was wasted was used as the main material to prepare whey protein film, and used for cherry fruit preservation. [ Result] The best formula membrane was solid-to-liquid 1: 8, glycerol additive amount 0. 2%, drying temperature 80 ℃. After the cherry tomatoes were coating by whey protein,the storage quality at 20 ℃ was improved significantly. [Conclusion] Whey protein film could slow down the cherry tomatoes become decay, weight loss, soft,and it had better effect on preservation. Whey protein film which was added potassium sorbate had better fresh effect.

  5. Antioxidant Activity and Phenolic Content of Sweet Cherries (Prunus Avium L. from West and South-West of Romania

    Directory of Open Access Journals (Sweden)

    Sofia Popescu

    2014-11-01

    Full Text Available A diet rich in fruits and vegetables is associated with a lower incidence of degenerative diseases (such as cardiovascular disease and certain types of cancers. Currently, most research is focused on the content of polyphenols and antioxidant compounds found in fruit and vegetable. Sweet cherries (Prunus avium L. contain a significant amount of polyphenols and several antioxidants that possess many biological activities such as anticancer, antioxidant and anti-inflammation properties. In present study were investigated the quantification of total polyphenols and antioxidant capacity in fruits of a number of selected sweet cherry genotypes. Although sweet cherry fruits are a significant source of different phenolic compounds, antioxidant activity of sweet cherries is not related only with the total phenolic content.  

  6. Tart cherry anthocyanins inhibit tumor development in Apc(Min) mice and reduce proliferation of human colon cancer cells.

    Science.gov (United States)

    Kang, Soo-Young; Seeram, Navindra P; Nair, Muraleedharan G; Bourquin, Leslie D

    2003-05-08

    Anthocyanins, which are bioactive phytochemicals, are widely distributed in plants and especially enriched in tart cherries. Based on previous observations that tart cherry anthocyanins and their respective aglycone, cyanidin, can inhibit cyclooxygenase enzymes, we conducted experiments to test the potential of anthocyanins to inhibit intestinal tumor development in Apc(Min) mice and growth of human colon cancer cell lines. Mice consuming the cherry diet, anthocyanins, or cyanidin had significantly fewer and smaller cecal adenomas than mice consuming the control diet or sulindac. Colonic tumor numbers and volume were not significantly influenced by treatment. Anthocyanins and cyanidin also reduced cell growth of human colon cancer cell lines HT 29 and HCT 116. The IC(50) of anthocyanins and cyanidin was 780 and 63 microM for HT 29 cells, respectively and 285 and 85 microM for HCT 116 cells, respectively. These results suggest that tart cherry anthocyanins and cyanidin may reduce the risk of colon cancer.

  7. The effect of the time of budding of mahaleb cherry (Prunus mahaleb L. seedlings on the quality of maiden trees of sour cherry (Prunus cerasus L. 'Łutówka'

    Directory of Open Access Journals (Sweden)

    Piotr Baryła

    2012-12-01

    Full Text Available The present study was conducted at the Felin Experi- mental Farm, belonging to the University of Life Sciences in Lublin, during the period 2005–2008. The experimental material consisted of maiden trees of sour cherry 'Łutówka' budded on seedlings of mahaleb cherry (Prunus mahaleb L. of unknown origin. The experiment evaluated the effect of four budding times: 15 July, 1 August, 15 August, and 1 September, on the quality of cherry trees in a nursery. The mean for the three years showed that budding time did not have a significant effect on the quality of cherry trees in the nursery. It was observed that the budding of mahaleb cherry performed on the two August dates (1st and 15th had a more beneficial effect on the growth and branching of trees than the budding done on 15 July and 1 September. The quality of maiden cherry trees 'Łutówka' in the nursery was primarily dependent on weather conditions in a given growing season, which is evidenced by the significant differences between production cycles, high variation in the quantitative results in individual years, and the absence of significant differences in the mean for 2006–2008.

  8. Cadmium accumulation in different rice cultivars and screening for pollution-safe cultivars of rice

    Energy Technology Data Exchange (ETDEWEB)

    Yu Hui [State Key Laboratory for Biocontrol, also School of Life Sciences, Sun Yat-sen University, Guangzhou 510275 (China); Wang Junli [State Key Laboratory for Biocontrol, also School of Life Sciences, Sun Yat-sen University, Guangzhou 510275 (China); Fang Wei [Biology Department, Long Island University, Brooklyn, NY 11201 (United States); Yuan Jiangang [State Key Laboratory for Biocontrol, also School of Life Sciences, Sun Yat-sen University, Guangzhou 510275 (China); Yang Zhongyi [State Key Laboratory for Biocontrol, also School of Life Sciences, Sun Yat-sen University, Guangzhou 510275 (China)]. E-mail: adsyzy@mail.sysu.edu.cn

    2006-11-01

    Large areas of contaminated land are being used for agricultural production in some countries due to the high demand for food. To minimize the influx of pollutants to the human food chain through consumption of agricultural products, we propose the concept of pollution-safe cultivars (PSCs), i.e. cultivars whose edible parts accumulate a specific pollutant at a level low enough for safe consumption, even when grown in contaminated soil. We tested the feasibility of the PSC concept by growing 43 cultivars of paddy rice (Oryza sativa L., including 20 normal and 23 hybrid cultivars) under a high (75.69-77.55 mg kg{sup -1}) and a low (1.75-1.85 mg kg{sup -1}) cadmium (Cd) exposure. These pot experiments took place in the spring and summer of 2004. At the low level of Cd exposure, 30 out of the 43 tested cultivars were found to be Cd-PSCs. Grain Cd concentrations were highly correlated (p < 0.01) between the two experiments, suggesting that Cd accumulation in rice grain is genotype-dependent and that the selection of PSCs is possible, at least at a certain level of soil contamination. No Cd-PSCs were found under the high level of Cd exposure. Yield was enhanced in some cultivars and depressed in others in response to elevated soil Cd, indicating that farmers cannot rely on yield depression as an indicator of toxicity of the grains. It is therefore important and feasible to screen for PSCs and to establish PSC breeding programs to effectively and efficiently reduce the risk of human exposure to soil pollutants, such as Cd, through crop consumption.

  9. Characterization of oats (Avena sativa L.) cultivars using machine vision.

    Science.gov (United States)

    Sumathi, S; Balamurugan, P

    2013-10-15

    Machine vision or image analysis is an important tool in the study of morphology of any materials. This technique has been used successfully to differentiate the eleven oats cultivars based on morphological characters. The geometry of seeds was measured through image analyzer and the variation was observed and recorded. From the recorded data, the cluster analysis was carried out and it revealed that the cultivars could be grouped into two main clusters based on similarity in the measured parameters. Cultivar Sabzar, UPO 212, OL 9 and OL 88 formed one main cluster. The another main cluster includes cv. Kent, OS 6, UPO 94, HFO 114, OS 7, HJ 8 and JHO 822 with many sub clusters. Among the cultivars HJ 8 and JHO 822 has more similarity in all measured parameters than other cultivars. Thus morphological characterization through seed image analysis was found useful to discriminate the cultivars.

  10. High-resolution seismic reflection/refraction imaging from Interstate 10 to Cherry Valley Boulevard, Cherry Valley, Riverside County, California: implications for water resources and earthquake hazards

    Science.gov (United States)

    Gandhok, G.; Catchings, R.D.; Goldman, M.R.; Horta, E.; Rymer, M.J.; Martin, P.; Christensen, A.

    1999-01-01

    This report is the second of two reports on seismic imaging investigations conducted by the U.S. Geological Survey (USGS) during the summers of 1997 and 1998 in the Cherry Valley area in California (Figure 1a). In the first report (Catchings et al., 1999), data and interpretations were presented for four seismic imaging profiles (CV-1, CV-2, CV-3, and CV-4) acquired during the summer of 1997 . In this report, we present data and interpretations for three additional profiles (CV-5, CV-6, and CV-7) acquired during the summer of 1998 and the combined seismic images for all seven profiles. This report addresses both groundwater resources and earthquake hazards in the San Gorgonio Pass area because the shallow (upper few hundred meters) subsurface stratigraphy and structure affect both issues. The cities of Cherry Valley and Beaumont are located approximately 130 km (~80 miles) east of Los Angeles, California along the southern alluvial fan of the San Bernardino Mountains (see Figure 1b). These cities are two of several small cities that are located within San Gorgonio Pass, a lower-lying area between the San Bernardino and the San Jacinto Mountains. Cherry Valley and Beaumont are desert cities with summer daytime temperatures often well above 100 o F. High water usage in the arid climate taxes the available groundwater supply in the region, increasing the need for efficient management of the groundwater resources. The USGS and the San Gorgonio Water District (SGWD) work cooperatively to evaluate the quantity and quality of groundwater supply in the San Gorgonio Pass region. To better manage the water supplies within the District during wet and dry periods, the SGWD sought to develop a groundwater recharge program, whereby, excess water would be stored in underground aquifers during wet periods (principally winter months) and retrieved during dry periods (principally summer months). The SGWD preferred a surface recharge approach because it could be less expensive than a

  11. Determination of optimal excitation and emission wavebands for detection of defect cherry tomato by using fluorescence emission and excitation matrix

    Science.gov (United States)

    Baek, In-Suck; Cho, Byoung-Kwan; Kim, Moon S.; Kim, Young-Sik

    2013-05-01

    Fluorescence imaging technique has been widely used for quality and safety measurements of agro-food materials. Fluorescence emission intensities of target materials are influenced by wavelengths of excitation sources. Hence, selection of a proper excitation wavelength is an important factor in differentiating target materials effectively. In this study, optimal fluorescence excitation wavelength was determined on the basis of fluorescence emission intensity of defect and sound areas of cherry tomatoes. The result showed that fluorescence responses of defect and sound surfaces of cherry tomatoes were most significantly separated with the excitation light wavelength range between 400 and 410 nm. Fluorescence images of defect cherry tomatoes were acquired with the LEDs with the central wavelength of 410 nm as the excitation source to verify the detection efficiency of cherry tomato defects. The resultant fluorescence images showed that the defects were discriminated from sound areas on cherry tomatoes with above 98% accuracy. This study shows that high power LEDs as the excitation source for fluorescence imaging are suitable for defect detection of cherry tomatoes.

  12. Screening of Carnation Cultivars for Resistance to Meloidogyne incognita

    OpenAIRE

    Cho, M. R.; Kim, J. Y.; Song, C.; Ko, J.Y.; Na, S. Y.; Yiem, M. S.

    1996-01-01

    A total of 33 carnation cultivars cultured in Korea were screened for resistance to the southern root-knot nematode, Meloidogyne incognita. Carnations were tested by either inoculating with 5,000 eggs or by transplanting into a mixture of bedding medium and soil infested with an average of 435 second-stage juveniles/300 cm³ soil. Cultivars, Desio, Castelaro, Kappa, Rara, Izu Pink, Target, and Antalia were highly resistant to M. incognita. Twelve cultivars were moderately resistant, and the re...

  13. Morphological characteristic of purple long yard bean cultivars and their tolerance to drought stress

    Directory of Open Access Journals (Sweden)

    M W Lestari

    2015-01-01

    Full Text Available The cultivation of purple long yard bean which tolerance to drought stress and have high productivity can improve farming in arid area. The purpose of this study was to evaluate the mechanism of the tolerance purple long yard beans to drought stress based on morphologic characters, to get the hypothesis method of tolerance and to obtain tolerance cultivars to the drought stress. Eight cultivars of purple long yard beans, i.e. UBPHU1-41, UBPHU1-130, UBPU3-153, UBPU1-202, UBPU2-222, UBPU1-365, Brawijaya 4 and Bagong 2, were tested in two environmental conditions, 100% field capacity and 50% field capacity. The results showed that drought stress in purple long yard bean affected all morphological characters observed, except for root length and flowering time. Estimation of tolerance to drought stress using the Principles Component Analysis (PCA showed that the shoot fresh weight could be an indicator of purple pod bean tolerance to drought stress. However, the test using Stress Susceptibility Index (SSI was not able to classify the purple long yard bean tolerance to drought stress. The results of analysis using PCA followed by discriminant analysis and clustering dendrogram showed that the UBPU1-41, UBPU1-130, UBPU2-222, UBPU1-365, UB4 and Bagong 2 cultivars were medium cultivars that are tolerant to drought stress. Therefore, they can be planted in semiarid regions.

  14. Nitrogen fertilization of fall panicum cultivars (Panicum dichotomiflorum Michx.: biochemical and agronomical aspects

    Directory of Open Access Journals (Sweden)

    Soratto Rogério Peres

    2004-01-01

    Full Text Available Information on improved fall panicum cultivars and agronomical pratices is scarce because of the relatively small importance of this crop in Brazil. The present study aimed to evaluate levels of nitrogen sidedressing on plant development and biochemical characteristics of two fall panicum cultivars. The experiment was set up under plastic tunnel conditions in Botucatu, São Paulo, Brazil, with plants growing inside asbestos-cement boxes. The experimental design was a randomized block setup and treatments consisted of six levels of nitrogen fertilization (0, 40, 80, 120, 160, and 200 mg L-1 applied at the onset of tillering on two fall panicum cultivars (AL Mogi and AL Tibagi. The application of increasing levels of nitrogen to fall panicum plants increased chlorophyll content, total amino acids, plant dry matter nitrogen content, plant height, tillering and grain yield, and decreased plant dry matter sugar content. The cultivars had similar performances in relation to the evaluated variables, as a response to nitrogen sidedressing.

  15. Brassinosteroids protect photosynthetic machinery against the cadmium induced oxidative stress in two tomato cultivars.

    Science.gov (United States)

    Hasan, Syed Aiman; Hayat, Shamsul; Ahmad, Aqil

    2011-09-01

    The present study was conducted with an aim to gain better insight of brassinosteroid generated response on the effects of cadmium on photosynthetic machinery and active oxygen metabolism in two tomato cultivars (K-25 and Sarvodya). These tomato cultivars were subjected to graded cadmium levels in soil (0, 3, 6, 9 or 12 mg kg(-1) soil) with their foliage being sprayed with 0 or 10(-8) M of 28-homobrassinolide/24-epibrassinolide (HBL/EBL) at 59 d stage. The results suggested that photosynthetic parameters, leaf water potential and activity of several enzymes (nitrate reductase and carbonic anhydrase) decreased significantly in both the cultivars, to a lesser extent in K-25 than Sarvodya with the increasing levels of cadmium in the soil. However, the activity of antioxidant enzymes and proline content increased in response to metal treatment as well as the application of brassinosteroids (HBL/EBL). Overall, exogenous application of brassinosteroids improved the activity of photosynthetic machinery and that of antioxidant defense system in both the cultivars, and also nullified the damaging effect of metal on these parameters.

  16. [Effects of ecological factors on the dough extensograph parameters of different winter wheat cultivars].

    Science.gov (United States)

    Zhang, Xue-lin; Wang, Chen-yang; Guo, Tian-cai; Wang, Yong-hua; Zhu, Yun-ji

    2009-12-01

    In 2000-2001 and 2001-2002, six representative winter wheat cultivars Yumai 34, Gaomai 8901, Yumai 49, Yumai 70, Luoyang 8716, and Yumai 50 were consecutively grown at five locations (Xinyang, Zhumadian, Xuchang, Wuzhi, and Tangyin) with latitudes varying from 32 degrees N to 36 degrees N in Henan Province, aimed to understand the relationships of winter wheat dough extensograph parameters with genetic and ecological factors. The dough extensograph parameters were more affected by genetic factors than by ecological factors. Cultivars Yumai 34 and Gaomai 8901 had significantly higher maximum resistance and extension area than the other four test cultivars, and significant differences in the dough extensograph parameters were observed between the cultivars grown in the south region (Xinyang and Zhumadian) and in the north region (Wuzhi and Tangyin) of the Province. The change patterns of dough extensograph parameters with latitude differed in 2000-2001 and in 2001-2002, and the effects of climatic factors on the dough extensograph parameters varied with year. In 2001-2002, the precipitation at the stage from grain-filling to maturing affected the dough extensograph parameters significantly. Our results suggested that in order to improve the dough extensograph parameters of winter wheat, local meteorological conditions should be taken into full consideration in the soil water management at late-maturing stage.

  17. Effect of HMW-GS 5+10 on Quality Parameters in Four Leading Wheat Cultivars

    Institute of Scientific and Technical Information of China (English)

    ZHANG Yan-bin; SUN Lian-fa; XIN Wen-li; SONG Qin-jie; ZHANG Chun-li; ZHAO Hai-bin; XIAO Zhi-min; QI Shi-yu

    2003-01-01

    HMW-GS 5+ 10 was introduced to four major wheat cultivars Kehan9, Kefeng3, Longmai20and Kenda4 through 5 or 6 consecutive backcrosses. No significant difference in protein content and dry glutencontent was observed between cultivars containing 5+ 10 and 2+ 12 or 3+12 (P>0. 1). In cuitivars contai-ning HMW-GS 5 +10, the ratio of wet gluten content to dry gluten content was 2.9 -5.0 % (P<0.01) lowerthan the ratio from the cultivars containing 2+ 12 or 3+12, the ratio of Zeleny sedimentation volume to drygluten content was 4.5 - 13.4% (P<0. 05) higher. The degree of softening in cuitivars having 5+10 was 15- 25 FU (P<0.01) lower, while the maximum resistance was 82 - 193 EU (P<0.05) higher. Backcrosseswith biochemical marker assisted selection in the improvement of quality in wheat cultivars was discussed.

  18. Composite Appreciation of Fruit Characters in Sweet Cherry and Sour Cherry Cultivars%甜樱桃和酸樱桃品种果实性状的综合评价

    Institute of Scientific and Technical Information of China (English)

    高佳; 王宝刚; 冯晓元; 李文生; 张开春; 汤浩茹

    2011-01-01

    以‘早大果’、‘红灯’等14个生产上早栽培甜樱桃品种和1个酸樱桃品种‘CAB'为试材,研究了不同品种间形态性状、内在品质和主要内含物含量的差异显著性,并对各项测定指标进行相关性检验.结果表明:15个樱桃品种以单果均重、总酸、还原糖、可溶性固形物、花色苷、总酚和维生素C含量差异最明显,可见樱桃果实大小、风味和内含物含量受品种影响显著.相关性检验显示,总酚与总酸;花色苷与还原糖、可溶性固形物;果形指数与总酸、pH值;单果均重与单核均重;果实汁液色泽与糖酸、可溶性固形物、花色苷和总酚呈显著相关关系.

  19. Efeito da autofecundação em cultivares de abacaxi Effects of self pollination in pineapple cultivars

    Directory of Open Access Journals (Sweden)

    José Renato Santos Cabral

    2003-04-01

    pineapple breeding program in conduction at Embrapa Cassava and Fruit Crops. On the other hand, the effects of self fertilization are not well known on pineapple, however this strategy may lead to significant advances in the improvement of that crop. The objective of this work was to evaluate the effects of self pollination in pineapple cultivars. Inflorescences of the cultivars Primavera, Perolera, Roxo-de-Tefé, Pérola and Smooth Cayenne were covered before anthesis in order to promote self fertilization. The resulting seeds were transferred to Petri dishes containing MS medium supplemented with 30 g.L-1 for germination and incubed in growth chamber. During germination it was observed that 16% of the seeds from 'Roxo-de-Tefe' generated albino plantlets. Forty three plants from 'Primavera', five from 'Perolera', eleven from 'Roxo-de-Tefé' and none from 'Pérola' and 'Smooth Cayenne' were obtained. All plants from Primavera presented spineless leaf margin (piping type, suggesting homozygosis for that characteristics, while in the 'Perolera" progeny three plants showed spineless leaf margin and two showed spiny leaves, showing segregation for the character presence of spine. Regarding to 'Roxo-de-Tefé' progeny, eight plants showed violet colored leaves and three with green colored leaves, expressing segregation for leaf color, but all leaves were spiny. The low percentage of germination, slow growth rate and low vigor observed in plants kept under greenhouse conditions as well as nursery, indicate the occurrence of depression by endogamy during these developmental phases.

  20. Photosynthetic Diurnal Variation of Soybean Cultivars with High Photosynthetic Efficiency

    Institute of Scientific and Technical Information of China (English)

    MAN Wei-qun; DU Wei-guang; ZHANG Gui-ru; LUAN Xiao-yan; GE Qiao-ying; HAO Nai-bin; CHEN Yi

    2002-01-01

    The photosynthetic characters were investigated among soybean cultivars with high photosynthetic efficiency and high yield. The results indicated that: 1) There were significant differences in photosynthetic rate (Ph) and dark respiration rate (DR) under saturation light intensity and appropriate temperature.2) There were a little difference in light compensation point among them. Photo flux density (PFD) were mong the cultivars. Diurnal variation of Pn was shown a curve with two peaks. 4) The cultivars with high photosynthetic efficiency were subjected less to photoinhibition than that with high yield. Critical temperatures of photoinhibition in high photosynthetic efficiency cultivars were higher than that of high yield.

  1. Rational regional distribution of sugarcane cultivars in China.

    Science.gov (United States)

    Luo, Jun; Pan, Yong-Bao; Xu, Liping; Grisham, Michael Paul; Zhang, Hua; Que, Youxiong

    2015-10-26

    Knowing yield potential and yield stability of sugarcane cultivars is of significance in guiding sugarcane breeding and rationalising regional distribution of sugarcane cultivars. In the present study, a heritability-adjusted genotype main effect plus genotype × environment (HA-GGE) biplot program was used to analyze the cane and sucrose yields of 44 newly released sugarcane cultivars at eight pilot test sites. The cane and sucrose yields of nine cultivars were higher than those of the control cultivar ROC22. From the perspective of cane yield, cultivars FN 40 and YZ 06-407 were well adapted to a wider range of conditions and produced relatively high cane yields in several pilot sites. From the perspective of sucrose yield, cultivars LC 03-1137, FN 38, FN 41, MT 01-77 and LC 05-136 were well adapted to a wide range of conditions and produced relatively high sucrose yields. Based on these results, three high yielding and widely adapted cultivars, namely, FN 39, LC 05-136, and YZ 05-51 were recommended for production in three major Chinese sugarcane planting areas. The results will provide a theoretical basis for recommending the effective use and rational regional distribution of sugarcane cultivars in China.

  2. Within-plant distribution of cotton aphid (Hemiptera: Aphididae in cotton cultivars with colored fibers

    Directory of Open Access Journals (Sweden)

    Francisco S. Fernandes

    2012-09-01

    Full Text Available We describe the vertical and horizontal distribution of the cotton aphid Aphis gossypii Glover within a cotton plant in two cotton (Gossypium hirsutum Linnaeus cultivars (BRS Safira and BRS Rubí with colored fiber over the time. Measurements of aphid population dynamics and distribution in the cotton plants were recorded in intervals of seven days. The number of apterous or alate aphids and their specific locations were recorded, using as a reference point the location of nodes on the mainstem of the plant and also those on the leaves present on branches and fruit structures. The number of apterous aphids found on the cultivar BRS Safira (56,515 aphids was greater than that found on BRS Rubí (50,537 aphids. There was no significant difference between the number of alate aphids found on the cultivars BRS Safira (365 aphids/plant and BRS Rubí (477 aphids/plant. There were interactions between cotton cultivar and plant age, between plant region and plant age, and between cultivar and plant region for apterous aphids. The results of this study are of great importance in improving control strategies for A. gossypii in the naturally-colored cotton cultivars BRS Safira and BRS Rubí.Nós descrevemos a distribuição vertical e horizontal do pulgão do algodoeiro Aphis gossypii Glover dentro da planta de algodão (Gossypium hirsutum Linnaeus, em dois cultivares com fibras coloridas (BRS Safira and BRS Rubí ao longo do seu desenvolvimento. Medidas de dinâmicas de populações e distribuição de A. gossypii nas plantas de algodão foram registradas em intervalos de sete dias. O número de afídeos ápteros ou alados e suas localizações específicas foram registrados, usando-se como ponto de referência a localização do nó no caule principal da planta e também aqueles presentes nas folhas de ramos e estruturas frutíferas. O número de afídeos ápteros encontrados na cultivar BRS Safira (56.515 afídeos foi maior do que o encontrado na BRS Rub

  3. Yield and water use efficiency of different irrigated sugarcane cultivars in Brazil

    Science.gov (United States)

    Silva, André L. B. O.; Pires, Regina C. M.; Ohashi, Augusto Y. P.; Ribeiro, Rafael V.

    2015-04-01

    There is an increasing demand for bioenergy production to provide environmental, economic and agricultural sustainability. In this context Brazil has an important option with sugarcane cultivation. The sugarcane cultivation has been increasing in marginal and appropriate areas depending on weather conditions. In appropriate areas, such as the State of São Paulo, it is important to increase yield and quality instead of expanding new areas. In this context, irrigation becomes an important cultural practice as a guarantee and to achieve high yields. Thus, the use of subsurface drip irrigation (SDI) in sugarcane cultivation is an interesting cultural practice to save water since water and nutrients are applied in root zone plants. As irrigation demands great volume of water, it is important to study the most responsive cultivars to adopt this technique and improve water use efficiency (WUE). Thus, this study aimed to evaluate the yield and WUE of four sugarcane cultivars irrigated by a SDI system. The experiment with the SP79-1011, IACSP94-2101, IACSP94-2094 and IACSP95-5000 cultivars was carried out in Campinas, SP, Brazil, between October 2012 and November 2013 (second ratoon). These cultivars have different canopy characteristics and development. IACSP95-5000 and IACSP94-2094 are more responsive to soil water availability and presents higher light interception when compared to IACSP94-2101 and SP79-1011. The irrigation was applied by a subsurface drip system daily and it was suspended when precipitation occurred. Crop evapotranspiration was estimated through field water balance. In order to do so the soil moisture was evaluated with capacitance probe with sensors installed at depths of 0.2, 0.3, 0.4, 0.6, 0.8 and 1.0 m. Samplings were collected to estimate yield and qualitative attributes. The water use efficiency (WUE) was estimated based on stem production per hectare reached in each cultivar divided by (1) water volume contributed considering the actual

  4. Aceitação de cultivares de bananas resistentes à Sigatoka Negra junto ao consumidor da região Nordeste do Brasil Acceptance of banana cultivars resistant to Black Sigatoka by the consumer market of Northeast Brazilian region

    Directory of Open Access Journals (Sweden)

    Deborah dos Santos Garruti

    2012-05-01

    ' and 'Pacovan' cultivars without impairing their global acceptability, but 'Pacovan Ken' still needs to be improved regarding its appearance.

  5. Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmer-preferred cassava cultivars.

    Science.gov (United States)

    Nyaboga, Evans; Njiru, Joshua; Nguu, Edward; Gruissem, Wilhelm; Vanderschuren, Herve; Tripathi, Leena

    2013-01-01

    Cassava genetic transformation capacity is still mostly restricted to advanced laboratories in the USA, Europe and China; and its implementation and maintenance in African laboratories has remained scarce. The impact of transgenic technologies for genetic improvement of cassava will depend largely on the transfer of such capabilities to researchers in Africa, where cassava has an important socioeconomic niche. A major constraint to the development of genetic transformation technologies for cassava improvement has been the lack of an efficient and robust transformation and regeneration system. Despite the success achieved in genetic modification of few cassava cultivars, including the model cultivar 60444, transgenic cassava production remains difficult for farmer-preferred cultivars. In this study, a protocol for cultivar 60444 developed at ETH Zurich was successfully implemented and optimized to establish transformation of farmer-preferred cassava cultivars popular in east Africa. The conditions for production and proliferation of friable embryogenic calli (FEC) and Agrobacterium-mediated transformation were optimized for three east African farmer-preferred cultivars (Ebwanatereka, Kibandameno and Serere). Our results demonstrated transformation efficiencies of about 14-22 independent transgenic lines per 100 mg of FEC for farmer-preferred cultivars in comparison to 28 lines per 100 mg of the model cultivar 60444. The presence, integration and expression of the transgenes were confirmed by PCR, Southern blot analysis and histochemical GUS assay. This study reports the establishment of a cassava transformation platform at International Institute of Tropical Agriculture (IITA) hosted by Biosciences eastern and central Africa (BecA) hub in Kenya and provides the basis for transferring important traits such as virus resistance and prolonged shelf-life to farmer-preferred cultivars in east Africa. We anticipate that such platform will also be instrumental to transfer

  6. Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmer-preferred cassava cultivars

    Directory of Open Access Journals (Sweden)

    Evans eNyaboga

    2013-12-01

    Full Text Available Cassava genetic transformation capacity is still mostly restricted to advanced laboratories in the USA, Europe and China; and its implementation and maintainance in African laboratories has remained scarce. The impact of transgenic technologies for genetic improvement of cassava will depend largely on the transfer of such capabilities to researchers in Africa, where cassava has an important socioeconomic niche. A major constraint to the development of genetic transformation technologies for cassava improvement has been the lack of an efficient and robust transformation and regeneration system. Despite the success achieved in genetic modification of few cassava cultivars, including the model cultivar 60444, transgenic cassava production remains difficult for farmer-preferred cultivars. In this study, a protocol for cultivar 60444 developed at ETH Zurich was successfully implemented and optimized to establish transformation of farmer-preferred cassava cultivars popular in east Africa. The conditions for production and proliferation of friable embryogenic calli (FEC and Agrobacterium-mediated transformation were optimized for three east African farmer-preferred cultivars (Ebwanatereka, Kibandameno and Serere. Our results demonstrated transformation efficiencies of about 14-22 independent transgenic lines per 100 mg of FEC for farmer-preferred cultivars in comparison to 28 lines per 100 mg of the model cultivar 60444. The presence, integration and expression of the transgenes were confirmed by PCR, Southern blot analysis and histochemical GUS assay. This study reports the establishment of a cassava transformation platform at International Institute of Tropical Agriculture (IITA hosted by Biosciences eastern and central Africa (BecA hub in Kenya and provides the basis for transferring important traits such as virus resistance and prolonged shelf-life to farmer-preferred cultivars in east Africa. We anticipate that such platform will also be

  7. Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus acidophilus.

    Science.gov (United States)

    Mohedano, M Luz; García-Cayuela, Tomás; Pérez-Ramos, Adrián; Gaiser, Rogier A; Requena, Teresa; López, Paloma

    2015-02-01

    Lactobacilli are widespread in natural environments and are increasingly being investigated as potential health modulators. In this study, we have adapted the broad-host-range vector pNZ8048 to express the mCherry protein (pRCR) to expand the usage of the mCherry protein for analysis of gene expression in Lactobacillus. This vector is also able to replicate in Streptococcus pneumoniae and Escherichia coli. The usage of pRCR as a promoter probe was validated in Lactobacillus acidophilus by characterizing the regulation of lactacin B expression. The results show that the regulation is exerted at the transcriptional level, with lbaB gene expression being specifically induced by co-culture of the L. acidophilus bacteriocin producer and the S. thermophilus STY-31 inducer bacterium.

  8. Physicochemical characterisation of four cherry species (Prunus spp.) grown in China.

    Science.gov (United States)

    Cao, Jinping; Jiang, Qing; Lin, Juanying; Li, Xian; Sun, Chongde; Chen, Kunsong

    2015-04-15

    The physicochemical characteristics of four cherry species (Prunus avium, Prunus cerasus, Prunus pseudocerasus and Prunus tomentosa) were evaluated. Inter-species variability was greater than intra-species differences. Glucose and fructose were the main sugars, and malic acid was the main organic acid in all species. Combining HPLC-DAD and LC-ESI-MS/MS technologies, total 25 phenolic components were preliminarily identified. P. avium was characterised by high fruit weight, edible proportion, sugar content and low acid content, which made it suitable for fresh eating. P. cerasus was high in acid content and anthocyanins content, making it a good processing species. P. pseudocerasus had rich flavonols varieties and high proportion of hydrocinnamic acids. P. tomentosa was characterised by high total phenolics content (especially flavonols and tannins) and antioxidant activity, indicating a great developmental potential as a health fruit. The results of the present study might provide theoretical guidance for the further development and utilisation of cherries.

  9. Effects of Different Atmospheres on the Postharvest Physiology and Quality of the Sweet Cherry

    Institute of Scientific and Technical Information of China (English)

    JIANG Ai-li; TIAN Shi-ping; XU Yong; WANG Yi; FAN Qing

    2002-01-01

    The changes in physiological characteristics, quality and storability of the sweet cherry (Prumus avium L. cv. Hongdeng) stored in controlled atmospheres (CA), in modified atmosphere packages (MAP) and in air (CK) were investigated in this paper. The results showed that CA and MAP treatments significantly inhibited fruit rot and flesh browning, kept firmness and fruit color, reduced ethylene and ethanol content in pulp, slowed down the increase of polyphenol oxidase (PPO) and peroxidase (POD) activity and malondialdehyde (MDA) content in comparison with CK. Meanwhile, CA treatments showed a better benefit of reducing ethylene and ethanol contents, inhibiting PPO and POD activities, declining rot rate and browning index compared to MAP. The fruit could be stored in CA conditions for 60 days without any off-flavor. The sweet cherries kept in CA with 5% O2 + 10% CO2 showed a better storability than that in CA with 5% O2 +5% CO2.

  10. Optimization of Sour Cherry Juice Spray Drying as
Affected by Carrier Material and Temperature

    Science.gov (United States)

    Zorić, Zoran; Pedisić, Sandra; Dragović-Uzelac, Verica

    2016-01-01

    Summary Response surface methodology was applied for optimization of the sour cherry Marasca juice spray drying process with 20, 30 and 40% of carriers maltodextrin with dextrose equivalent (DE) value of 4–7 and 13–17 and gum arabic, at three drying temperatures: 150, 175 and 200 °C. Increase in carrier mass per volume ratio resulted in lower moisture content and powder hygroscopicity, higher bulk density, solubility and product yield. Higher temperatures decreased the moisture content and bulk density of powders. Temperature of 200 °C and 27% of maltodextrin with 4–7 DE were found to be the most suitable for production of sour cherry Marasca powder. PMID:28115901

  11. Benzaldehyde in cherry flavour as a precursor of benzene formation in beverages.

    Science.gov (United States)

    Loch, Christine; Reusch, Helmut; Ruge, Ingrid; Godelmann, Rolf; Pflaum, Tabea; Kuballa, Thomas; Schumacher, Sandra; Lachenmeier, Dirk W

    2016-09-01

    During sampling and analysis of alcohol-free beverages for food control purposes, a comparably high contamination of benzene (up to 4.6μg/L) has been detected in cherry-flavoured products, even when they were not preserved using benzoic acid (which is a known precursor of benzene formation). There has been some speculation in the literature that formation may occur from benzaldehyde, which is contained in natural and artificial cherry flavours. In this study, model experiments were able to confirm that benzaldehyde does indeed degrade to benzene under heating conditions, and especially in the presence of ascorbic acid. Analysis of a large collective of authentic beverages from the market (n=170) further confirmed that benzene content is significantly correlated to the presence of benzaldehyde (r=0.61, pbenzaldehyde in combination with ascorbic acid should be avoided.

  12. Common bean cultivars response to lime surface application under no tillage systemResposta de cultivares de feijoeiro comum à calagem superficial em semeadura direta

    Directory of Open Access Journals (Sweden)

    Carlos Alexandre Costa Crusciol

    2012-08-01

    Full Text Available Soil acidity in no tillage system, if not in high situations, can be neutralized by lime surface application, improving mineral nutrition and crop yield. Aiming to evaluate the agronomic performance of common bean cultivars, to surface lime application, in no tillage system, an experiment was conducted in Oxisol, Botucatu Municipal District, São Paulo State, Brazil. The experimental design was a complete randomized block in split plot with four replications, where the plots were formed by common bean cultivars (Carioca, IAC Carioca Eté, Pérola, IAPAR 81 e Campeão 2 and subplots consisted of surface application of dolomitic limestone (zero, 1.8 t ha-1, 3.6 t ha-1 and 5.4 t ha-1. The surface lime application on the soil occurred in October 2002 and subsequently the sequence millet (spring – beans (summer – oat (autumn-winter were planted under rainfed conditions. Bean cultivars sowing were done on December 17, 2003. It can be concluded that there is influence of cultivars and limestone surface application under no tillage, where IAPAR 81 showed better grain yield with the increase of lime rates, obtaining values of 2,025 kg ha-1 without the lime application to 2,655 kg ha-1 with 5.4 t ha-1 lime rate, obtaining 31% yield increase. A acidez do solo no sistema de semeadura direta, caso não se encontre em situações elevadas, pode ser resolvida com aplicação superficial de calcário, melhorando a nutrição mineral e a produtividade das culturas. Com o objetivo de avaliar o desempenho agronômico de cultivares de feijoeiro, em razão da aplicação superficial de calcário, em semeadura direta, foi realizado um experimento num Latossolo Vermelho distrófico, em Botucatu (SP. O delineamento experimental foi o de blocos casualizados, em esquema de parcelas subdivididas com quatro repetições, onde as parcelas foram formadas por cultivares de feijão comum (Carioca, IAC Carioca Eté, Pérola, IAPAR 81 e Campeão 2 e as subparcelas constitu

  13. Desempenho de cultivares de jiló em casa de vegetação Performance of jilo cultivars in glasshouse conditions

    Directory of Open Access Journals (Sweden)

    Maria do Carmo de Salvo Soares Novo

    2008-01-01

    picked and their diameter, length and fresh biomass were measured. 'Esmeralda' and 'Comprido Verde-Claro' showed premature fruit production when compared to Bernacci and Cardoso cultivars. The latter cultivars yielded fruits for longer period of time. Cardoso cultivar produced a bigger number and a larger total fresh fruit biomass than the other genotypes. Bernacci and Cardoso cultivars of IAC germoplasm collection showed to be genotypes with potential to be used in genetic improvement, in familiar agriculture and in the system of support production and they are the traditional ways of jilo cultivation.

  14. CULTIVAR RELEASE - FAEM Carlasul: new white oat cultivar with high grain yield

    Directory of Open Access Journals (Sweden)

    Antônio Costa de Oliveira

    2012-01-01

    Full Text Available The white oat cultivar FAEM Carlasul was developed at the Plant Genomics and Breeding Center, Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas, as a result of the cross between UFRGS 10 and 90SAT-28 (Coronado2/Cortez3/Pendek/ME 1563. It is characterized by high yield and grain quality.

  15. CULTIVAR RELEASE-BRS 217 Flora: Early-maturing soybean cultivar

    Directory of Open Access Journals (Sweden)

    Plínio Itamar de Mello de Souza

    2008-01-01

    Full Text Available The soybean (Glycine max L. Merr. cultivar BRS 217 Flora was developed by Embrapa and released forproduction in the states of Goiás, Minas Gerais, Bahia, Mato Grosso and the Distrito Federal, Brazil. It is resistant to stemcanker, frog-eye leaf spot, bacterial pustule, and partially resistant to powdery mildew.

  16. Teores de proteína, óleo, lisina e triptofano em grãos integrais de diversos cultivares de arroz Oil, protein, lysine, and triptophan content of some rice cultivars

    Directory of Open Access Journals (Sweden)

    J. P. F. Teixeira

    1976-07-01

    Full Text Available Tendo em vista futuros programas de melhoramento da qualidade nutritiva do arroz, foram determinados os teores de óleo, proteína e dos aminoácidos essenciais lisina e triptofano nos grãos descascados dos principais cultivares desse cereal cultivados no Estado de São Paulo. Foram encontradas diferenças estatisticamente significativas entre os teores desses constituintes, nos diferentes cultivares estudados. A interação variedade-ambiente teve efeito marcante nos teores de proteína dos grãos, com exceção do IAC-47. De uma maneira geral, os teores de lisina e de triptofano foram mais elevados nos materiais que apresentaram teores mais baixos de proteína. Dentre os principais cultivares de arroz atualmente em plantio, o IAC-47 apresentou características promissoras para futuros programas de melhoramento visando melhoria da qualidade nutricional desse cereal.The purpose of this study was to determine the content of oil, protein, lysine, and triptophan of 12 rice cultivars of the State of São Paulo. These data are of large interest as a support for the future rice breeding program in order to improve the nutritional value of this cereal. Significant differences were found for all the characteristics caused by vari-etal effects. Environmental factors, such as the year and the location where the rice grew, severely affected the protein content of all the cultivars, but for the IAC-47. In general, the low protein cultivars showed higher levels of lysine and triptophan. The IAC-47 cultivar revealed the most stable chemical composition and very promising to be used in the breeding for high-protein rices.

  17. Storage performance of Taiwanese sweet potato cultivars

    OpenAIRE

    Huang, Che-Lun; Liao, Wayne C.; Chan, Chin-Feng; Lai, Yung-Chang

    2013-01-01

    Three sweet potato cultivars (TNG57, TNG66, and TNG73), provided by the Taiwanese Agricultural Research Institute (TARI), were stored at either 15 °C or under ambient conditions (23.8 ~ 28.4 °C and 77.1 ~ 81.0 % of relative humidity). Sweet potato roots were randomly chosen from each replicate and evaluated for measurement of weight loss, sugar content analysis, and sprouting after 0, 14, 24, 48, 56, 70, 84, and 98 days of storage. Fresh sweet potato roots were baked at 200 °C for 60 min then...

  18. Nutritional properties of cherry tomatoes harvested at different times and grown in an organic cropping.

    OpenAIRE

    Pinho, L.; Almeida, A. C.; Costa, C.A.; PAES, M. C. D.; M.B.A. Glória; Souza, R. M.

    2011-01-01

    The physicochemical characteristics of the cherry tomato cultivated in organic and conventional production systems and harvested at either 30 or 45 days of cropping were evaluated using a randomized, 2x2 factorial design (2 cropping systems x 2 harvesting times) with five repetitions. The parameters analyzed were color, centesimal composition, total energetic value, carotenoids and bioactive amine content. Tomatoes harvested at 30 days had higher total soluble solid (TSS) content when grown c...

  19. Habitat-specific flight period in the cherry fruit fly Rhagoletis cingulata (Loew) (Diptera: Tephritidae).

    Science.gov (United States)

    Teixeira, Luís A F; Isaacs, Rufus; Gut, Larry J

    2007-12-01

    Flight periods of the cherry fruit fly, Rhagoletis cingulata (Loew), were compared in the major sweet and tart cherry-growing regions of Michigan, among neglected orchards, managed orchards, and natural areas containing the ancestral host, black cherry. Traps were deployed from early June to late September 2005 and 2006. Captures indicated that cherry fruit fly has an early flight (June-July) in neglected orchards, a mid-season flight peaking immediately after harvest (June-August) in managed orchards, and an extended flight covering most of the season (June-September) in natural areas. We found that the period of fruit infestation mirrored the flight period in neglected and managed orchards. In natural areas, we found infestation late in the season only. The relative emergence periods for adults reared from pupae collected from the three habitats and maintained under the same conditions coincided with adult flight periods for each habitat. We also studied factors related to fruit availability that may have a role in shaping the flight periods. Fruit abundance decreased rapidly early in the season in neglected orchards, whereas in managed orchards, fruit left after harvest remained on the trees until late August. Measurements of fruit size and skin firmness revealed that fly activity in neglected and managed orchards began immediately after fruit increased in size and skin firmness decreased, whereas in natural areas, the flight began before fruit matured. In managed orchards, fruit harvest and insecticide sprays likely maintain the late flight period of resident fly populations by preventing the use of fruit earlier in the season. However, a significant proportion of these resident flies may still emerge before harvest and increase the risk of costly fruit infestation.

  20. Assessing the potential for establishment of western cherry fruit fly using ecological niche modeling.

    Science.gov (United States)

    Kumar, Sunil; Neven, Lisa G; Yee, Wee L

    2014-06-01

    Sweet cherries, Prunus avium (L.) L., grown in the western United States are exported to many countries around the world. Some of these countries have enforced strict quarantine rules and trade restrictions owing to concerns about the potential establishment and subsequent spread of western cherry fruit fly, Rhagoletis indifferens Curran (Diptera: Tephritidae), a major quarantine pest of sweet cherry. We used 1) niche models (CLIMEX and MaxEnt) to map the climatic suitability, 2) North Carolina State University-Animal and Plant Health Inspection Service Plant Pest Forecasting System to examine chilling requirement, and 3) host distribution and availability to assess the potential for establishment of R. indifferens in areas of western North America where it currently does not exist and eight current or potential fresh sweet cherry markets: Colombia, India, Indonesia, Malaysia, Taiwan, Thailand, Venezuela, and Vietnam. Results from niche models conformed well to the current distribution of R. indifferens in western North America. MaxEnt and CLIMEX models had high performance and predicted climatic suitability in some of the countries (e.g., Andean range in Colombia and Venezuela, northern and northeastern India, central Taiwan, and parts of Vietnam). However, our results showed no potential for establishment of R. indifferens in Colombia, Indonesia, Malaysia, Taiwan, Thailand, Venezuela, and Vietnam when the optimal chilling requirement to break diapause (minimum temperature < or = 3 degree C for at least 15 wk) was used as the criterion for whether establishment can occur. Furthermore, these countries have no host plant species available for R. indifferens. Our results can be used to make scientifically informed international trade decisions and negotiations by policy makers.

  1. TEXTURAL, FLOW AND SENSORY PROPERTIES OF FIVE “FRUZELINA” WITH SOUR CHERRIES

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    Irena Bojdo Tomasiak

    2010-05-01

    Full Text Available Gel with sour cherries called “Fruzelina” is a new product in the Polish market widely used in food industry as a decorative element or filling for pastries, as an ingredient in fruit desserts, as an additive to ice creams, whipped cream and waffles. The cherry gels are the product prepared using different types of chemically modified starches. Starch is an additive used to ensure rich and short texture and high viscosity of “Fruzelina”. Food texture and viscosity may be measured by senses and instrumentally. Because of fact that sensory analysis is time consuming and very costly, it is easier and cheaper to determine food properties, especially their texture and flow behaviour by appropriate mechanical tests. The aim of this work was to study the rheological behavior of five cherry gels and evaluate the correlation between textural, flow and sensory properties of these gels measured instrumentally and by human senses. The back extrusion test has been found to be applicable to study the textural properties of cherry gels. There was high positive correlation between gel texture measured by senses and texture parameters measured in back extrusion test. Similar high correlation was identified for consistency coefficient K obtained in Ostwald de Waele model and gel texture assessed by sensory panel. It was found that values of sensory parameters such as taste and odour decreased as the rheological parameters increased. High negative correlations were observed in these cases. Therefore, instrumental measurements can be alternative for more expensive sensory methods. doi:10.5219/53

  2. Production of bacterial cellulose by Gluconacetobacter hansenii UAC09 using coffee cherry husk

    OpenAIRE

    Rani, M. Usha; Appaiah, K. A. Anu

    2011-01-01

    The work is aimed to investigate the suitability of underutilized coffee cherry husk (CCH) for the production and optimization of bacterial cellulose (BC) by Gluconacetobacter hansenii UAC09 and to study the physico-mechanical properties of BC films. CCH extract was used as a carbon source in various concentrations along with other nutritional components such as nitrogen (corn steep liquor, urea) and additives (ethyl alcohol, acetic acid). Concentration of CCH extract at 1:1 (w/v) along with ...

  3. Yield and quality of strawberry cultivars Produtividade e qualidade de cultivares de morangueiro

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    Luis Eduardo C Antunes

    2010-06-01

    Full Text Available The strawberry cultivation is an important economic activity in the Rio Grande do Sul State, Brazil, however the number of offered cultivars to the growers is reduced. The yield and quality of strawberry (Fragaria x ananassa Duch. cultivars Camarosa, Galexia, Earlibrite, Festival, Plarionfre and Sabrosa was evaluated under the climatic conditions of Pelotas, Rio Grande do Sul State. We determined the number, mass, total soluble solids (TSS ºBrix, total titratable acidity (TTA, antocianin level and fresh fruits produced in an experimental unit. We also determined the production of fruits per hectare and per plant. The statistical design used in the experiment was of completely randomized blocks with 6 treatments (cultivars and 4 replicates where the experimental unit was composed of 8 plants. The harvest began in the first half of August, extending to the second half of December, totalling 20 weeks. Plarionfre, Earlibrite and Festival cultivars showed higher production from the first half of October until the end of the first half of November. Camarosa reached higher productivity, mass of plants and fruit weight. There were no differences between the evaluated cultivars in the levels of TSS, antocianin, ATT and pH during the period of evaluation.No estado do Rio Grande do Sul o cultivo do morangueiro é uma atividade econômica importante, entretanto o número de cultivares disponíveis no mercado é bastante reduzido. Assim buscou-se com este trabalho avaliar a produtividade e a qualidade dos frutos de cultivares de morangueiro (Fragaria x ananassa Duch., Camarosa, Galexia, Earlibrite, Festival, Plarionfre e Sabrosa, cultivados nas condições climáticas e de solo do município de Pelotas, RS. Determinaram-se, o número, massa fresca e teor de sólidos solúveis totais (SST (expresso em ºBrix, acidez total titulável (ATT (expresso em % de ácido cítrico e teor de antocianinas, dos frutos produzidos por unidade experimental. Determinou

  4. Sensibilidade de cultivares de algodoeiro ao cloreto de mepiquat Sensibility of cotton cultivars to mepiquat chloride

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    Julio Cesar Bogiani

    2009-10-01

    Full Text Available O objetivo deste trabalho foi avaliar a sensibilidade de cultivares de algodoeiro ao cloreto de mepiquat e verificar se ela está relacionada ao ciclo ou à arquitetura da planta. Foi realizado um experimento em casa de vegetação com seis cultivares de algodoeiro - FiberMax 966, FiberMax 977, Delta Penta, Delta Opal, FMT 501 e FMT 701 - e quatro doses do regulador vegetal à base de cloreto de mepiquat - 0, 7,5, 15 e 22,5 g ha-1 de i.a. -, em delineamento de blocos ao acaso, com arranjo fatorial 6x4 e quatro repetições. As parcelas experimentais foram constituídas por vasos de 12 L com duas plantas em cada um. As doses do regulador vegetal foram aplicadas sem adjuvante, no estádio de aparecimento dos botões florais, aos 34 dias após a emergência das plântulas. Paralelamente ao experimento com doses, a retenção do regulador pelas folhas das cultivares foi quantificada. O crescimento em altura das plantas de algodoeiro é diminuído com a aplicação do regulador vegetal, e esse efeito se intensifica com o aumento da dosagem aplicada. Existe diferença entre as cultivares estudadas quanto à sensibilidade ao cloreto de mepiquat, sendo que as mais precoces são mais sensíveis. Além da sensibilidade diferenciada, a arquitetura das plantas pode possibilitar maior ou menor deposição do regulador sobre as folhas.The objective of this work was to assess the sensibility of cotton cultivars to mepiquat chloride and to verify if it is related to plant cycle or architecture. A green-house experiment was carried out with six cotton cultivars - FiberMax 966, FiberMax 977, Delta Penta, Delta Opal, FMT 501 e FMT 701 -, and four concentrations of mepiquat chloride plant growth regulator - 0, 7,5, 15 and 22,5 g ha-1 of the active ingredient - in complete block design with a 6x4 factorial arrangement and four replicates. The plots consisted of 12 L pots with two plants per pot. Regulator doses were applied without adjuvants at the beginning of

  5. Coherences of instrumental and sensory characteristics: case study on cherry tomatoes.

    Science.gov (United States)

    Csambalik, László; Divéky-Ertsey, Anna; Pap, Zoltán; Orbán, Csaba; Stégerné Máté, Mónika; Gere, Attila; Stefanovits-Bányai, Éva; Sipos, László

    2014-11-01

    The aim of this study was to investigate 6 cherry tomato varieties in terms of morphological, instrumental, and sensory attributes. Hungarian cherry tomato landraces have not been investigated in comparison with new commercial varieties for these traits. Parameters investigated were water-soluble antioxidant capacity (FRAP, DPPH, and TEAC), and total polyphenol, vitamin C, β-carotene, lycopene, total soluble solids, and acid contents. Colorimetric measurements as well as sensory analyses were conducted. It was concluded that varied antioxidant assays should be used in parallel to overcome the selectivity of any 1 method. Total phenolic content significantly contributed to results of antioxidant assays for the investigated varieties. The sensory profiles of the 6 cherry tomato varieties have been created. The differences between the products based on the 18 attributes were analyzed by Tukey post hoc test. The biplot of the principal component analysis showed that the sensory panel could discriminate the samples along the principal components. No correlation was found between colorimetric data a* and b* measured from pulp and lycopene, but a negative connection of β-carotene and hue was noted. Total polyphenol content showed correlations with colorimetric results, except for b*. The influence of tomato skin color on color perception is significant as in the present study instrumental data measured from pulp did not match that of the panelists evaluating intact fruit. Instrumental results of sugar content were supported by the ratings of the sensory panel.

  6. Phenolic compounds in cherry ( Prunus avium ) heartwood with a view to their use in cooperage.

    Science.gov (United States)

    Sanz, Miriam; Cadahía, Estrella; Esteruelas, Enrique; Muñoz, Angel Ma; Fernández De Simón, Brígida; Hernández, Teresa; Estrella, Isabel

    2010-04-28

    The phenolic and tannic composition of heartwood extracts from Prunus avium , commonly known as cherry tree, before and after toasting in cooperage were studied using HPLC-DAD and HPLC-DAD/ESI-MS. Nonflavonoid (16 compounds) and flavonoid (27 compounds) polyphenols were identified, 12 of them in only a tentative way. The nonflavonoids found were lignin constituents, and their pattern is different compared to oak, since they include compounds such as protocatechuic acid and aldehyde, p-coumaric acid, methyl vanillate, methyl syringate, and benzoic acid, but not ellagic acid, and only a small quantity of gallic acid. In seasoned wood we found a great variety of flavonoid compounds which have not been found in oak wood for cooperage, mainly, in addition to the flavan-3-ols (+)-catechin, a B-type procyanidin dimer, and a B-type procyanidin trimer, the flavanones naringenin, isosakuranetin, and eriodictyol and the flavanonols aromadendrin and taxifolin. Seasoned and toasted cherry wood showed different ratios of flavonoid to nonflavonoid compounds, since toasting results in the degradation of flavonoids, and the formation of nonflavonoids from lignin degradation. On the other hand, the absence of hydrolyzable tannins in cherry wood, which are very important in oak wood, is another particular characteristic of this wood that should be taken into account when considering its use in cooperage.

  7. High concentrations of anthocyanins in genuine cherry-juice of old local Austrian Prunus avium varieties.

    Science.gov (United States)

    Schüller, Elisabeth; Halbwirth, Heidi; Mikulic-Petkovsek, Maja; Slatnar, Ana; Veberic, Robert; Forneck, Astrid; Stich, Karl; Spornberger, Andreas

    2015-04-15

    Antioxidant activity and polyphenols were quantified in vapour-extracted juice of nine Austrian, partially endemic varieties of sweet cherry (Prunus avium): cv. 'Spätbraune von Purbach', cv. 'Early Rivers', cv. 'Joiser Einsiedekirsche', cv. 'Große Schwarze Knorpelkirsche' and four unidentified local varieties. Additionally the effect of storage was evaluated for six of the varieties. A variety showing the highest antioxidant capacity (9.64 μmol Trolox equivalents per mL), total polyphenols (2747 mg/L) and total cyanidins (1085 mg/L) was suitable for mechanical harvest and its juice did not show any losses of antioxidant capacity and total anthocyanin concentration during storage. The juice of cv. 'Große Schwarze Knorpelkirsche' had also high concentrations of total anthocyanins (873 mg/L), but showed substantial losses through storage. The local Austrian sweet cherry varieties from the Pannonian climate zone are particularly suitable for the production of processed products like cherry juice with high content of anthocyanins and polyphenols.

  8. Cultivation of cherry tomato under irrigation with saline water and nitrogen fertilization

    Directory of Open Access Journals (Sweden)

    Ianne G. S. Vieira

    2016-01-01

    Full Text Available ABSTRACT The study was carried out from August 2013 to January 2014 to evaluate growth and production of cherry tomato cultivated under irrigation with water of different salinity levels and fertilized with different nitrogen (N doses, in experiment conducted in drainage lysimeters under greenhouse conditions, at the Center for Agrifood Science and Technology of the Federal University of Campina Grande. The statistical design was randomized blocks in a 5 x 4 factorial scheme, with three replicates, and the treatments consisted of five levels of electrical conductivity of water (0.3, 1.5, 2.5, 3.5 and 4.5 dS m-1 and four N doses (60, 100, 140 and 180 mg kg-1. Growth and production variables of cherry tomato decrease linearly from the irrigation water salinity of 0.3 dS m-1 on. The longer exposure of plants to salt stress caused the highest reductions, and the root dry matter, leaf area and the number of clusters are the most sensitive variables. The highest value of plant height at 125 days after transplantation was obtained with the N dose of 139 mg kg-1 of soil. Increasing N doses reduced the effect of salinity on cherry tomato growth at 125 days after transplantation.

  9. Selection of autochthonous sour cherry (Prunus cerasus L. genotypes in Feketić region

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    Radičević Sanja

    2012-01-01

    Full Text Available Autochthonous genotypes of fruit species are very important source of genetic variability and valuable material for breeding work. Fruit Research Institute-Čačak has a long tradition of studying autochthonous genotypes of temperate fruits sporadically spread and preserved in some localities in Serbia. Over 2005-2006, the following properties of nine autochthonous sour cherry genotypes grown in Feketic region were investigated: flowering and ripening time, pomological properties, biochemical composition of fruits and field resistance to causal agents of cherry diseases - cherry leaf spot (Blumeriella jaapii (Rehm. v. Arx., shot-hole (Clasterosporium carpophilum (Lév. Aderh. and brown rot (Monilinia laxa /Ader et Ruhl./ Honey ex Whetz.. The genotypes were tested for the presence of Prune dwarf virus and Prunus necrotic ring spot virus. In majority of genotypes fruits were large, with exceptional organoleptical properties, whereas ripening time was in the first ten or twenty days of June. The highest fruit weight was observed in F-1 genotype (8.1 g. The highest soluble solids and total sugars content were found in F- 4 genotype (17.60% and 14.25%, respectively. As for field resistance to causal agents of diseases and good pomo-technological properties, F-1, F-2, F-3, F-7 and F-8 genotypes were singled out. [Projekat Ministarstva nauke Republike Srbije, br. TR31064

  10. In vitro sterilization procedures for micropropagation of ‘oblačinska’ sour cherry

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    Mihaljević Ines

    2013-01-01

    Full Text Available Surface sterilization is the most important step in preparation of explants for micropropagation, because controlling fungal and bacterial contamination of woody plant from field sources is very difficult. Six sterilizing agents: sodium hypochlorite (NaOCl, calcium hypochlorite [Ca(ClO2], sodium dichloroisocyanurate (DICA, mercuric (II chloride (HgCl2, silver nitrate (AgNO3 and hydrogen peroxide (H2O2 were tested for sterilization of ‘Oblačinska’ sour cherry buds, by varying their concentration and time of exposure. The aim of this study was to establish best surface sterilization for in vitro propagation of ‘Oblačinska’ sour cherry. Aseptic cultures of ‘Oblačinska’ sour cherry were established from axillary buds which were placed in nutrient medium, supplemented with plants hormones 6-benzylaminopurine (BA, 1- naphthaleneacetic acid (NAA and gibberellic acid (GA3. The results indicated that among these sterilizing agents silver nitrate (AgNO3 at concentration of 1% for 20 minutes was the best for controlling the infection, whereas sterilization with sodium dichloroisocyanurate (DICA at concentration of 1% for 10 minutes was not satisfactory.

  11. Genetic diversity analysis reveals that geographical environment plays a more important role than rice cultivar in Villosiclava virens population selection.

    Science.gov (United States)

    Wang, Fei; Zhang, Shu; Liu, Mei-Gang; Lin, Xian-Song; Liu, Hui-Jiang; Peng, You-Liang; Lin, Yang; Huang, Jun-Bin; Luo, Chao-Xi

    2014-05-01

    Rice false smut caused by Villosiclava virens is an economically important disease of grains worldwide. The genetic diversity of 153 isolates from six fields located in Wuhan (WH), Yichang Wangjia (YCW), Yichang Yaohe (YCY), Huanggang (HG), Yangxin (YX), and Jingzhou (JZ) in Hubei province of China were phylogenetically analyzed to evaluate the influence of environments and rice cultivars on the V. virens populations. Isolates (43) from Wuhan were from two rice cultivars, Wanxian 98 and Huajing 952, while most of the other isolates from fields YCW, YCY, HG, YX, and JZ originated from different rice cultivars with different genetic backgrounds. Genetic diversity of isolates was analyzed using random amplified polymorphic DNA (RAPD) and single-nucleotide polymorphisms (SNP). The isolates from the same cultivars in Wuhan tended to group together, indicating that the cultivars had an important impact on the fungal population. The 110 isolates from individual fields tended to cluster according to geographical origin. The values of Nei's gene diversity (H) and Shannon's information index (I) showed that the genetic diversity among isolates was higher between than within geographical populations. Furthermore, mean genetic distance between groups (0.006) was higher than mean genetic distance within groups (0.0048) according to MEGA 5.2. The pairwise population fixation index (FST) values also showed significant genetic differentiation between most populations. Higher genetic similarity of isolates from individual fields but different rice cultivars suggested that the geographical factor played a more important role in the selection of V. virens isolates than rice cultivars. This information could be used to improve the management strategy for rice false smut by adjusting the cultivation measures, such as controlling fertilizer, water, and planting density, in the rice field to change the microenvironment.

  12. Variation in broccoli cultivar phytochemical content under organic and conventional management systems: implications in breeding for nutrition.

    Science.gov (United States)

    Renaud, Erica N C; Lammerts van Bueren, Edith T; Myers, James R; Paulo, Maria João; van Eeuwijk, Fred A; Zhu, Ning; Juvik, John A

    2014-01-01

    Organic agriculture requires cultivars that can adapt to organic crop management systems without the use of synthetic pesticides as well as genotypes with improved nutritional value. The aim of this study encompassing 16 experiments was to compare 23 broccoli cultivars for the content of phytochemicals associated with health promotion grown under organic and conventional management in spring and fall plantings in two broccoli growing regions in the US (Oregon and Maine). The phytochemicals quantified included: glucosinolates (glucoraphanin, glucobrassicin, neoglucobrassin), tocopherols (δ-, γ-, α-tocopherol) and carotenoids (lutein, zeaxanthin, β-carotene). For glucoraphanin (17.5%) and lutein (13%), genotype was the major source of total variation; for glucobrassicin, region (36%) and the interaction of location and season (27.5%); and for neoglucobrassicin, both genotype (36.8%) and its interactions (34.4%) with season were important. For δ- and γ-tocopherols, season played the largest role in the total variation followed by location and genotype; for total carotenoids, genotype (8.41-13.03%) was the largest source of variation and its interactions with location and season. Overall, phytochemicals were not significantly influenced by management system. We observed that the cultivars with the highest concentrations of glucoraphanin had the lowest for glucobrassicin and neoglucobrassicin. The genotypes with high concentrations of glucobrassicin and neoglucobrassicin were the same cultivars and were early maturing F1 hybrids. Cultivars highest in tocopherols and carotenoids were open pollinated or early maturing F1 hybrids. We identified distinct locations and seasons where phytochemical performance was higher for each compound. Correlations among horticulture traits and phytochemicals demonstrated that glucoraphanin was negatively correlated with the carotenoids and the carotenoids were correlated with one another. Little or no association between

  13. Reaction of Oilseed Rape Cultivars to Sclerotinia Stem Rot in Field Environments

    Institute of Scientific and Technical Information of China (English)

    SUN Jun-ming; HAN Fen-xia; Malgorzata Jedryczka

    2005-01-01

    Sclerotinia sclerotiorum (Lib.) de Bary, as an ubiquitous phytopathogenic Ascomycete fungus capable of infecting a wide range of plants, has increased in importance for oilseed rape production worldwide in recent years. The objective of this research was to determine the effectiveness of two measurements of disease rating in predicting the field reaction of cultivars to sclerotinia stem rot. One hundred and ten cultivars or lines of oilseed rape were repeatedly evaluated the disease severity index (DSI) and lesion length for their resistance to nine isolates of S. sclerotiorum in two field stations.There were greatly significant differences between two stations, among isolates, among population of cultivars for the disease severity index and lesion length, and also significant station×isolate interaction for lesion length and population×isolate interactions for DSI and lesion length, but interactions of station×isolate for DSI were not significant.Cultivars such as Pomorzanin and Lisek consistently had the lowest disease severity ratings in the field tests. However,most of cultivars were susceptible to sclerotinia stem rot in the field test. A positive correlation between the mean DSI and lesion length was found for both isolates (Sc23 and B1). The correlations ranged from 0.23 to 0.83 at various plots, with nine of the twelve correlations significant. The correlations for disease caused by different fungal isolates were statistically significant difference. These correlations ranged from -0.44 to 0.90, with nine of the thirty-two correlations significant. It indicated that resistance to some isolates was significantly correlated and selection of some varieties for resistance to one isolate appeared to improve resistance to other isolates.

  14. Genetic diversity among some canola cultivars as revealed by RAPD, SSR and AFLP analyses.

    Science.gov (United States)

    Moghaieb, Reda E A; Mohammed, Etr H K; Youssief, Sawsan S

    2014-08-01

    To assess the genetic diversity among four canola cultivars (namely, Serw-3, Serw-4, Misser L-16 and Semu 249), random amplified polymorphic DNA (RAPD), simple sequence repeat polymorphism (SSR) and amplified fragment length polymorphism (AFLP) analyses were performed. The data indicated that all of the three molecular markers gave different levels of polymorphism. A total of 118, 31 and 338 markers that show 61, 67.7 and 81 % polymorphism percentages were resulted from the RAPD, SSR and AFLP analyses, respectively. Based on the data obtained the three markers can be used to differentiate between the four canola cultivars. The genotype-specific markers were determined, 18 out of the 72 polymorphic RAPD markers generated were found to be genotype-specific (25 %). The highest number of RAPD specific markers was scored for Semu 249 (15 markers), while Serw-4 scored two markers. On the other hand, Serw-3 scored one marker. The cultivar Semu 249 scored the highest number of unique AFLP markers, giving 57 unique markers, followed by Misser L-16 which was characterized by 40 unique AFLP markers, then Serw-3 giving 31 unique markers. While Serw-4 was characterized by the lowest number producing 14 unique positive markers. The dendrogram built on the basis of combined data from RAPD, SSR and AFLP analysis represents the genetic distances among the four canola cultivars. Understanding the genetic variability among the current canola cultivars opens up a possibility for developing a molecular genetic map that will lead to the application of marker-assisted selection tools in genetic improvement of canola.

  15. Raising potential yield of short-duration rice cultivars is possible by increasing harvest index

    Directory of Open Access Journals (Sweden)

    Huang, M.

    2015-01-01

    Full Text Available Description of the subject. Further increases in rice yield potential are generally thought to require greater biomass assimilation. This study presents a new cultivar that draws greater yield from increased harvest index (HI. Objectives. Our objective was to identify the physiological traits that are critical to the high yield of a recently developed short-duration rice cultivar Guiliangyou 2 (GLY2. Method. GLY2 and a check cultivar Yuxiangyouzhan (YXYZ were grown in a field at the Experimental Farm of Guangxi University, Guangxi province, southern China in early and late rice-growing seasons in 2013. Grain yield, yield components, canopy characteristics including leaf area index (LAI, leaf N content (LNC, leaf area duration (LAD and assimilation rate (NAR, biomass accumulation, and harvest index were determined for each cultivar in each season. Results. GLY2 produced 17–38% higher grain yield than YXYZ with the same growth duration. Spikelets per m2 and grain weight were higher in GLY2 than in YXYZ by 11–13% and 6–17%, respectively. GLY2 had higher LAI and LAD but lower LNC and NAR than YXYZ. As a consequence of the compensation between the canopy characteristics, there was no significant difference in biomass accumulation between the two cultivars. Harvest index of GLY2 was 13–23% higher than that of YXYZ. Large sink size, high remobilization of stored reserves and maintained biomass production after heading were responsible for the high HI of GLY2. Conclusions. Our study suggests that it is possible to increase HI together with grain yield by improving the potential sink size and the remobilization of stored reserves while maintaining high LAI and LAD in short-duration rice.

  16. Altered hyperlipidemia, hepatic steatosis, and hepatic peroxisome proliferator-activated receptors in rats with intake of tart cherry.

    Science.gov (United States)

    Seymour, E Mitchell; Singer, Andrew A M; Kirakosyan, Ara; Urcuyo-Llanes, Daniel E; Kaufman, Peter B; Bolling, Steven F

    2008-06-01

    Elevated plasma lipids, glucose, insulin, and fatty liver are among components of metabolic syndrome, a phenotypic pattern that typically precedes the development of Type 2 diabetes. Animal studies show that intake of anthocyanins reduces hyperlipidemia, obesity, and atherosclerosis and that anthocyanin-rich extracts may exert these effects in association with altered activity of tissue peroxisome proliferator-activated receptors (PPARs). However, studies are lacking to test this correlation using physiologically relevant, whole food sources of anthocyanins. Tart cherries are a rich source of anthocyanins, and whole cherry fruit intake may also affect hyperlipidemia and/or affect tissue PPARs. This hypothesis was tested in the Dahl Salt-Sensitive rat having insulin resistance and hyperlipidemia. For 90 days, Dahl rats were pair-fed AIN-76a-based diets supplemented with either 1% (wt:wt) freeze-dried whole tart cherry or with 0.85% additional carbohydrate to match macronutrient and calorie provision. After 90 days, the cherry-enriched diet was associated with reduced fasting blood glucose, hyperlipidemia, hyperinsulinemia, and reduced fatty liver. The cherry diet was also associated with significantly enhanced hepatic PPAR-alpha mRNA, enhanced hepatic PPAR-alpha target acyl-coenzyme A oxidase mRNA and activity, and increased plasma antioxidant capacity. In conclusion, physiologically relevant tart cherry consumption reduced several phenotypic risk factors that are associated with risk for metabolic syndrome and Type 2 diabetes. Tart cherries may represent a whole food research model of the health effects of anthocyanin-rich foods and may possess nutraceutical value against risk factors for metabolic syndrome and its clinical sequelae.

  17. Effect of cultivar and year on phyllochron in winter barley

    Directory of Open Access Journals (Sweden)

    Pržulj Novo M.

    2013-01-01

    Full Text Available Development and growth of leaves in cereals significantly affects grain yield since dry matter accumulation depends on the leaf area that intercepts light. Phyllochron (PHY is defined as time interval between the emergences of successive leaves on the main stem. The aim of this study was to determine the effect of year and cultivar on phyllochron in winter barley. Twelve cultivars of winter barley differing in origin and time of anthesis were tested during six growing seasons (GS, from 2002/03 to 2007/08. The highest PHY across GSs was determined in the two-rowed cultivar Cordoba (81.6°Cd and the lowest in the two-rowed cultivar Novosadski 581 (71.0°Cd. The early cultivars had fast leaf development, the medium cultivars medium and the late cultivars slow development, 72.5°Cd, 75.6°Cd and 78.9°Cd, respectively. The tested cultivars showed significant variability in the PHY, which can be used for selecting most adaptable genotypes for specific growing conditions.

  18. Cultivar and row distance interactions in perennial ryegrass

    DEFF Research Database (Denmark)

    Deleuran, Lise C; Gislum, René; Boelt, Birte

    2009-01-01

    yield and seed weight (r=0.72***), whereas the correlation between seed yield and the number of reproductive tillers was negatively correlated (r= - 0.49***). This may reflect choice of cultivars in the experiment with the tetraploid forage cultivar 'Tivoli' having the lowest number of reproductive...

  19. Evaluation of Wheat and Barley Cultivars Tolerance to Metribuzine Application

    Directory of Open Access Journals (Sweden)

    E Izadi Darbandi

    2013-08-01

    Full Text Available In order to study of barely and wheat cultivars tolerance to metribuzin, a factorial experiment was conducted as a completely randomized design, with three replications in Greenhouse of Agricultural Research at Ferdowsi University of Mashhad. Treatments included wheat cultivars (Backcross roshan, Cross Arvand, Bahar, Sepahan, Gascosion, Sayonez, Bam garmsiry, Garmsiri, Ghods, Pishtaz, Chamran and Shoori 6, barely cultivars (Macouyi, Karoon and Bahman and metribuzin application rates ( 0, 175, 350, 700, 1050, 1400 and 2100 gr. ai.ha-1. Metribuzine was applied at 3-4 leaf stage and 3 weeks after herbicide spraying, plants survival and their biomass were determined. Results showed that metribuzin application had a significant effect (p≤0.01 on barley and wheat dry weight. Based on results, mertibuzin application did not affect on barley cultivars up to 30 g.a.i.ha-1 but in wheat varieties lead to significant reduction in their biomass and survival. Increasing of metribuzin rates reduced wheat and barley cultivars biomass (p≤0.01. Barely varieties were less sensitive than wheat cultivars to metribuzine. The highest and the lowest ED50 in wheat cultivars were observed in cross arvand (940 and shoori (25 varieties, respectively. In barley cultivars the highest and lowest ED50 were observed in Macouyi (614 and Karoon (396, respectively.

  20. Measurement of lipid transfer protein in 88 apple cultivars

    NARCIS (Netherlands)

    Sancho, A.I.; Ree, van R.; Leeuwen, van A.; Meulenbroek, E.J.; Weg, van de W.E.; Gilissen, L.J.W.J.; Puehringer, H.; Laimer, M.; Martinelli, A.; Zaccharini, M.; Vazquez-Cortes, S.; Fernandez-Rivas, M.; Hoffmann-Sommergruber, K.; Clare Mills, E.N.; Zuidmeer, L.

    2008-01-01

    Background: Fruits are a major cause of food allergy in adults. Lipid transfer proteins (LTP) are implicated in severe allergic reactions to fruits, but little is known about LTP content in different cultivars. Objective: Determination of the levels of LTP in a wide range of apple cultivars. Methods

  1. Identifying nitrogen-efficient potato cultivars for organic farming

    NARCIS (Netherlands)

    Tiemens-Hulscher, M.; Lammerts Van Bueren, E.; Struik, P.C.

    2014-01-01

    In organic farming, nitrogen efficiency of potato might vary among cultivars, even within the same maturity type. We therefore analysed in depth the response to nitrogen of a diverse set of cultivars, grown at different locations (differing in soil type and management) and in four years (differing i

  2. New Kiwifruit Cultivar Carves a Firm Niche in European Market

    Institute of Scientific and Technical Information of China (English)

    2004-01-01

    @@ While enhancing the world kiwifruit production by developing new cultivars,CAS researchers are gaining a firm foothold in international kiwifruit market as they prolong their control over the propagation rights of a recently developed novel yellowfleshed cultivar, Jin Tao, in European countries.

  3. Esterase polymorphism marking cultivars of Manihot esculenta, Crantz

    Directory of Open Access Journals (Sweden)

    Adriana Gazoli Resende

    2004-07-01

    Full Text Available Esterase isozymes were used to detected substrate-preference polymorphism in twenty cultivars of Manihot esculenta, and to show cultivar-specific variation of this species. A relatively complex extraction solution of proteins from leaves was needed to show a larger number of esterase isozymes. Similarity between cultivars from six groups ranged from 51 to 96%. The cultivars identified by the same name seemed to be biochemically different regarding esterase isozymes. Esterase isozyme electrophoretic patterns could, therefore, be used to discriminate the cultivars identified by the same name, and to monitor the vegetative propagation of cultivars maintained in the germplasm collection. In breeding strategies, isoesterase analysis could be used to avoid intercrossing between the similar genotypes.Isoenzimas esterases foram usadas no presente estudo, para detectar polimorfismos específicos para diferentes substratos em vinte cultivares de Manihot esculenta, e para mostrar variações específicas de cultivares nesta espécie. Os diferentes cultivares de M. esculenta tem sido mantidos na coleção de germoplasma do Departamento de Agronomia da Universidade Estadual de Maringá (Maringá, PR, e foram provenientes de cultivares tradicionais coletados nas regiões sudoeste e noroeste do Estado. Foi necessário a utilização de uma solução de extração de proteínas relativamente mais complexa, para evidenciar um maior número de isoenzimas esterases. A similaridade entre os cultivares variou de 51 a 96%. Cultivares identificados pelo mesmo nome parecem ser bioquimicamente diferentes para as isoenzimas esterases. Os padrões eletroforéticos das isoesterases podem, portanto, serem usados para discriminar os cultivares que são identificados pelo mesmo nome, e para monitorar a propagação vegetativa dos cultivares mantidos na coleção de germoplasma. A análise das isoesterases pode também ser usada para evitar cruzamentos entre genótipos mais

  4. Effects of gamma irradiation and/or cooking on nutritional quality of faba bean (Vicia faba L.) cultivars seeds.

    Science.gov (United States)

    Osman, Asha Mohamed Ali; Hassan, Amro B; Osman, Gammaa A M; Mohammed, Nagat; Rushdi, Mohamed A H; Diab, Eiman E; Babiker, Elfadil E

    2014-08-01

    The effect of gamma irradiation (0.5 and 1.0 kGy) and/or cooking on the proximate composition, mineral content, tannin content, phytic acid content and the in vitro protein digestibility (IVPD) of two Sudanese faba bean cultivars (BB7-S1 and SH-S2) was investigated in the present study. The results obtained revealed that gamma irradiation and/or cooking treatments have slight effect in chemical composition and mineral content, while they caused significant (P ≤ 0.05) reduction on tannin content for both cultivars. Cooking of faba bean seeds also insignificantly (P ≤ 0.05) reduced phytic acid content for both cultivars, while irradiation process and/or cooking had fluctuated effect. For both cultivars, irradiation of seeds and/or cooking increased the in vitro protein digestibility (IVPD), with maximum value of IVPD (79.97%) obtained for cultivar BB7-S1. The results indicate that the treatments used in this study might improve the nutritive quality of faba bean seed due to reduction in antinutritional factors with a concomitant increase in IVPD.

  5. Chilling and Host Plant/Site-Associated Eclosion Times of Western Cherry Fruit Fly (Diptera: Tephritidae) and a Host-Specific Parasitoid.

    Science.gov (United States)

    Yee, Wee L; Goughnour, Robert B; Hood, Glen R; Forbes, Andrew A; Feder, Jeffrey L

    2015-08-01

    The western cherry fruit fly, Rhagoletis indifferens Curran (Diptera: Tephritidae), is an endemic herbivore of bitter cherry, Prunus emarginata (Douglas ex Hooker) Eaton, but ∼100 years ago established on earlier-fruiting domesticated sweet cherry, Prunus avium (L.) L. Here, we determined if eclosion times of adult R. indifferens from sweet and bitter cherry differ according to the phenology of their respective host plants and if eclosion times of the host-specific parasitoid Diachasma muliebre (Muesebeck) (Hymenoptera: Braconidae) attacking bitter and sweet cherry flies differ according to the eclosion phenology of their fly hosts. Fly pupae from sweet and bitter cherry fruit were collected from sympatric and allopatric sites in Washington state, and chilled at 5°C. Because timing of eclosion in R. indifferens depends on chill duration, eclosion time in wasps could also vary with chill duration. To account for this, fly pupae were chilled for 1, 2, 2.5, 3, 4, 6, or 8 mo. Both flies and wasps eclosed earlier with longer chill durations. Eclosion times of sweet and bitter cherry flies from a sympatric site in central Washington did not differ. However, at allopatric sites in northwestern and central Washington, bitter cherry flies eclosed later than sweet and bitter cherry flies at the sympatric site. Correspondingly, D. muliebre parasitizing a more isolated bitter cherry fly population eclosed later than D. muliebre parasitizing earlier-emerging sweet and bitter cherry fly populations. These results provide evidence for D. muliebre rapidly responding to changes in host plant shifts by R. indifferens.

  6. Metabolomics-driven nutraceutical evaluation of diverse green tea cultivars.

    Directory of Open Access Journals (Sweden)

    Yoshinori Fujimura

    Full Text Available BACKGROUND: Green tea has various health promotion effects. Although there are numerous tea cultivars, little is known about the differences in their nutraceutical properties. Metabolic profiling techniques can provide information on the relationship between the metabolome and factors such as phenotype or quality. Here, we performed metabolomic analyses to explore the relationship between the metabolome and health-promoting attributes (bioactivity of diverse Japanese green tea cultivars. METHODOLOGY/PRINCIPAL FINDINGS: We investigated the ability of leaf extracts from 43 Japanese green tea cultivars to inhibit thrombin-induced phosphorylation of myosin regulatory light chain (MRLC in human umbilical vein endothelial cells (HUVECs. This thrombin-induced phosphorylation is a potential hallmark of vascular endothelial dysfunction. Among the tested cultivars, Cha Chuukanbohon Nou-6 (Nou-6 and Sunrouge (SR strongly inhibited MRLC phosphorylation. To evaluate the bioactivity of green tea cultivars using a metabolomics approach, the metabolite profiles of all tea extracts were determined by high-performance liquid chromatography-mass spectrometry (LC-MS. Multivariate statistical analyses, principal component analysis (PCA and orthogonal partial least-squares-discriminant analysis (OPLS-DA, revealed differences among green tea cultivars with respect to their ability to inhibit MRLC phosphorylation. In the SR cultivar, polyphenols were associated with its unique metabolic profile and its bioactivity. In addition, using partial least-squares (PLS regression analysis, we succeeded in constructing a reliable bioactivity-prediction model to predict the inhibitory effect of tea cultivars based on their metabolome. This model was based on certain identified metabolites that were associated with bioactivity. When added to an extract from the non-bioactive cultivar Yabukita, several metabolites enriched in SR were able to transform the extract into a bioactive

  7. ANTIOXIDANT AND ANTIMUTAGENIC ACTIVITIES OF TAIF GRAPE (VITIS VINIFERA CULTIVARS

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    Abdelmegid Ibrahim Fahmi

    2013-01-01

    Full Text Available Extract of grape Vitis vinifera has been reported to exhibit antioxidant and antimutagenic activities and the phenolic compounds play a vital role in determining these activities. Therefore; the objective of the present study was to evaluate the antioxidant and anti-mutagenic activities as well as the phenolic composition of different grape cultivar extracts collected from Taif region. The grape cultivars namely; Italian, American, Lebanese, Taifyb and Taifye were collected at maturity stage to represent Taif region cultivars. The total concentrations of phenoles were determined for the five cultivar extracts and results indicated that the concentrations ranged from 115-960 mg L-1 Gallic Acid Equivalent (GAE. Also, HPLC analysis included was carried out of nine important phenolic compounds namely; Cyanidine chloride, Myricetin, Chrysin, Quercetin, Delphinidine chloride, Malvidine chloride, Naringenin, Galangin and Caffeic acid. Significant differences among cultivars were obtained for each compound. However, the highest cultivar for each compound differed from compound to another. At the same time, DPPH was used to estimate antioxidant activity and the data showed that different grape cultivar extracts were able to quench 47-60% of DPPH radical solution and to exhibited potent radical scavenging activity. Also, antimutagenic activity was measured as a decrease of chromosomal aberrations in bone marrow cells of mice treated with the mutagen Endoxan. Results showed that treatment of mice with grape cultivar extracts resulted in a significant decrease in all types of chromosomal aberrations induced by Endoxan. Also, the anticlastogenic effect was measured using micronulei test and results indicated that all grape cultivar extracts reduced significantly the effect of Endoxan on micronulei test. Finally, treatment of mice with grape cultivar extracts enhanced mitotic index of mice bone marrow cells reduced by Endoxan treatment. The relationship

  8. A Functional mathematical index for predicting effects of food processing on eight sweet potato(Ipomoea batatas)cultivars

    Science.gov (United States)

    In this paper we apply an improved functional mathematical index (FMI), modified from those presented in previous publications, to define the influence of different cooking processes of eight sweet potato (Ipomoea batatas) cultivars on composition of six bioactive phenolic compounds (flavonoids). Th...

  9. Violet diode laser-induced chlorophyll fluorescence: a tool for assessing mosaic disease severity in cassava (Manihot esculenta Crantz) cultivars.

    Science.gov (United States)

    Anderson, Benjamin; Eghan, Moses J; Asare-Bediako, Elvis; Buah-Bassuah, Paul K

    2012-01-01

    Violet diode laser-induced chlorophyll fluorescence was used in agronomical assessment (disease severity and average yield per plant). Because cassava (Manihot esculenta Crantz) is of economic importance, improved cultivars with various levels of affinity for cassava mosaic disease were investigated. Fluorescence data correlated with cassava mosaic disease severity levels and with the average yield per plant.

  10. Variation in nutritional quality and chemical composition of fresh strawberry fruit: combined effect of cultivar and storage.

    Science.gov (United States)

    Dragišić Maksimović, Jelena; Poledica, Milena; Mutavdžić, Dragosav; Mojović, Miloš; Radivojević, Dragan; Milivojević, Jasminka

    2015-03-01

    Bioclimatic air ionisation system (BI) works by neutralising air pollutants and microorganisms by means of oxidation with "activated oxygen". We investigated the effects of storage on changes in weight loss, chemical and sensory fruit properties in eight cultivars of strawberries (Fragaria x ananassa Duch.). All cultivars were evaluated for their standard parameters of quality (soluble solids content, total acidity, vitamin C content, total antioxidant activity - TAC, total phenolic and anthocyanins content) at different store conditions: fresh fruits-control, cold stored (at 4 °C) fruits without controlled atmospheres and cold stored (at 4 °C) fruits in BI. The present study outlines that anthocyanins of the strawberries stored in BI were subjected to significant degradation. These strawberries have prolonged shelf-life accompanied by weight loss reduction, TAC increment, and sensory properties improvement in tested cultivars, retaining other nutritional fruit qualities.

  11. Field susceptibility of 13 scab-resistant apple cultivars to apple powdery mildew [Podosphaera leucotricha (Ell. et Ev. Salmon

    Directory of Open Access Journals (Sweden)

    Zbigniew Borecki

    2013-12-01

    Full Text Available Field susceptibility of 13 scab-resistant apple cultivars to apple powdery mildew was evaluated in 1983-1986. Four groups of susceptibility were distinguished. None of the 13 tested scab-resistant apple trees exhibited complete field immunity to apple powdery mildew. Two cultivars, 'Prima' and 'Primula', were practically resistant. 'Liberty' and two numbered selections, NY-140-9 and NY-158-2, belonged to the group of lower susceptibility. Moderate susceptibility was shown by: 'Novamac', 'Freedom', 'Gavin', 'Prima' and 'Florina'. The group of apple trees most susceptible to Podosphaera leucotricha included: 'Macfree', 'Priscilla' and 'Nova Easygro'. It is not necessary to use chemical sprays to control powdery mildew on 'Prima' and 'Primula'. A reduced spraying program may be recommended only under high disease pressure on less susceptible apple cultivars. A regular spray schedule is needed on moderately susceptible apple trees, but improved chemical control is necessary on the most susceptible ones.

  12. 甜樱桃(Prunus avium L.)品种S基因型鉴定%Identifying the S Genotypes of Sweet Cherry ( Prunus avium L.) Cultivars

    Institute of Scientific and Technical Information of China (English)

    陈晓流; 陈学森; 束怀瑞

    2004-01-01

    根据蔷薇科S-RNase基因(S基因)高度保守区C2和RC4区设计一对特异引物PruC2和PruC4R,对甜樱桃品种的基因组DNA进行S基因特异PCR扩增.克隆S基因的扩增片段,核酸序列在GenBank上搜索,确定了4种S基因的核酸序列和大小.结果表明,在琼脂糖凝胶上位置相同的扩增带其核酸序列相同,是同一种S基因.4种S基因扩增片段的大小分别是:S1为677 bp,S3为762 bp,S4为945 bp,S6为456 bp.参试的自交不亲和品种的S基因型分别是:红灯、红艳、早红宝石和先锋相同,为S1S3;抉择、红丰和那翁相同,为S3S4;大紫为S1S6;长把红为S1S4;养老为S3S6;自交亲和品种外引7号和斯太拉为S3S4'.

  13. 甜樱桃品种S基因型研究概况%General situation of studies on S genotypes of sweet cherry cultivars

    Institute of Scientific and Technical Information of China (English)

    艾呈祥; 张力思; 刘庆忠

    2007-01-01

    甜樱桃(Prunus avium L)属配子体型自交不亲和(Gametophyticself-incompatibility,GSI),遗传上由染色体上具有复等位基因构成的单一位点或基因座控制(称为S基因座)。在一个S基因座上,植物种群内可含有多个S等位基因,称为S基因。

  14. Implication of abscisic acid on ripening and quality in sweet cherries: differential effects during pre- and postharvest

    Directory of Open Access Journals (Sweden)

    Verónica eTijero

    2016-05-01

    Full Text Available Sweet cherry, a non-climacteric fruit, is usually cold-stored during postharvest to prevent over-ripening. The aim of the study was to evaluate the role of abscisic acid (ABA on fruit growth and ripening of this fruit, considering as well its putative implication in over-ripening and effects on quality. We measured the endogenous concentrations of ABA during the ripening of sweet cherries (Prunus avium L. var. Prime Giant collected from orchard trees and in cherries exposed to 4ºC and 23ºC during 10d of postharvest. Furthermore, we examined to what extent endogenous ABA concentrations were related to quality parameters, such as fruit biomass, anthocyanin accumulation and levels of vitamins C and E. Endogenous concentrations of ABA in fruits increased progressively during fruit growth and ripening on the tree, to decrease later during postharvest at 23ºC. Cold treatment, however, increased ABA levels and led to an inhibition of over-ripening. Furthermore, ABA levels positively correlated with anthocyanin and vitamin E levels during preharvest, but not during postharvest. We conclude that ABA plays a major role in sweet cherry development, stimulating its ripening process and positively influencing quality parameters during preharvest. The possible influence of ABA preventing over-ripening in cold-stored sweet cherries is also discussed.

  15. Implication of Abscisic Acid on Ripening and Quality in Sweet Cherries: Differential Effects during Pre- and Post-harvest

    Science.gov (United States)

    Tijero, Verónica; Teribia, Natalia; Muñoz, Paula; Munné-Bosch, Sergi

    2016-01-01

    Sweet cherry, a non-climacteric fruit, is usually cold-stored during post-harvest to prevent over-ripening. The aim of the study was to evaluate the role of abscisic acid (ABA) on fruit growth and ripening of this fruit, considering as well its putative implication in over-ripening and effects on quality. We measured the endogenous concentrations of ABA during the ripening of sweet cherries (Prunus avium L. var. Prime Giant) collected from orchard trees and in cherries exposed to 4°C and 23°C during 10 days of post-harvest. Furthermore, we examined to what extent endogenous ABA concentrations were related to quality parameters, such as fruit biomass, anthocyanin accumulation and levels of vitamins C and E. Endogenous concentrations of ABA in fruits increased progressively during fruit growth and ripening on the tree, to decrease later during post-harvest at 23°C. Cold treatment, however, increased ABA levels and led to an inhibition of over-ripening. Furthermore, ABA levels positively correlated with anthocyanin and vitamin E levels during pre-harvest, but not during post-harvest. We conclude that ABA plays a major role in sweet cherry development, stimulating its ripening process and positively influencing quality parameters during pre-harvest. The possible influence of ABA preventing over-ripening in cold-stored sweet cherries is also discussed. PMID:27200070

  16. Effect of a tart cherry juice supplement on arterial stiffness and inflammation in healthy adults: a randomised controlled trial.

    Science.gov (United States)

    Lynn, Anthony; Mathew, Shilpa; Moore, Chris T; Russell, Jean; Robinson, Emma; Soumpasi, Vithleem; Barker, Margo E

    2014-06-01

    Tart cherries are a particularly rich source of anthocyanins. Evidence indicates that dietary intake of anthocyanins is inversely associated with arterial stiffness. We conducted an open-label randomised placebo controlled study to determine whether a tart cherry juice concentrate (Cherry Active) reduced arterial stiffness, inflammation and risk markers for cardiovascular disease in 47 healthy adults (30-50 years). Participants consumed 30 ml of cherry concentrate diluted to a volume of 250 ml with water or the same volume of an energy matched control drink daily for six weeks. Measurements were taken at baseline and at the end of the intervention. There was no effect of the intervention on arterial stiffness (P = 0.218), c-reactive protein (P = 0.220), systolic blood pressure (P = 0.163), diastolic blood pressure (P = 0.121), total cholesterol (P = 0.342) and high density lipoprotein cholesterol (P = 0.127). At the end of the intervention, plasma antioxidant capacity (measured as the ferric reducing ability of plasma (FRAP)) was significantly higher in the intervention group than the control group (P = 0.012). We conclude that a tart cherry juice concentrate rich in anthocyanins has no effect on arterial stiffness, c-reactive protein and risk markers for cardiovascular disease, but evokes a minor increase in antioxidant status in healthy adults.

  17. Influence of Air Temperature and Pretreatment Solutions on Drying Time, Energy Consumption and Organoleptic Properties of Sour Cherry

    Directory of Open Access Journals (Sweden)

    Hamid Reza Gazor

    2014-09-01

    Full Text Available The effects of pretreatment solution (no treatment, boiling water, salty boiling water, ethil oleat on drying time of sour cherry were studied experimentally. The thin layer drying of sour cherries was carried out at three air temperatures of 50, 60, 70°C and with constant airflow velocity of 1 m/s. Drying kinetic, energy consumption and organoleptic properties as taste, visual color and texture were evaluated in dried fruits. Results of experiments showed that pretreatment solutions and air temperatures had significant effect on drying time and organoleptic properties of dried sour cherry. Using of pretreatment solution is necessary before drying process. It reduced drying time up to 80% and energy saving was approximately 83% in comparison with no treatment samples. Results of this research indicated that using of salty boiling water as pretreatment and temperature of 50°C in sour cherry drying process cause the best result in drying time and organoleptic evaluation such as taste quality, visual color and texture suitability of dried fruit. In addition, energy consumption for drying reduced noticeably when sour cherry was pretreated with salty boiling water.

  18. Influence of Air Temperature and Pretreatment Solutions on Drying Time, Energy Consumption and Organoleptic Properties of Sour Cherry

    Directory of Open Access Journals (Sweden)

    Hamid Reza Gazor

    2014-10-01

    Full Text Available The effects of pretreatment solution (no treatment, boiling water, salty boiling water, ethil oleat on drying time of sour cherry were studied experimentally. The thin layer drying of sour cherries was carried out at three air temperatures of 50, 60, 70°C and with constant airflow velocity of 1 m/s. Drying kinetic, energy consumption and organoleptic properties as taste, visual color and texture were evaluated in dried fruits. Results of experiments showed that pretreatment solutions and air temperatures had significant effect on drying time and organoleptic properties of dried sour cherry. Using of pretreatment solution is necessary before drying process. It reduced drying time up to 80% and energy saving was approximately 83% in comparison with no treatment samples. Results of this research indicated that using of salty boiling water as pretreatment and temperature of 50°C in sour cherry drying process cause the best result in drying time and organoleptic evaluation such as taste quality, visual color and texture suitability of dried fruit. In addition, energy consumption for drying reduced noticeably when sour cherry was pretreated with salty boiling water.

  19. Compatibilidade de enxertia de cultivares de marmeleiros com pereiras Compatibility of pear cultivars on quinces rootstocks

    Directory of Open Access Journals (Sweden)

    Zeni Fonseca Pinto Tomaz

    2009-12-01

    Full Text Available A insuficiência de estudos sobre compatibilidade de porta-enxertos é um dos fatores limitantes ao desenvolvimento da cultura da pereira (Pyrus sp. no Brasil. A utilização do marmeleiro (Cydonia oblonga como porta-enxerto para a cultura da pereira apresenta inúmeras vantagens, entre as quais a redução do vigor e a rápida entrada em produção; todavia, sua combinação com algumas cultivares copa apresenta problemas de incompatibilidade de enxertia, podendo ocasionar a ruptura do caule das plantas no pomar. Objetivou-se, neste trabalho, avaliar a compatibilidade de enxertia de algumas cultivares de marmeleiros ('Quince C' e 'Adams' com pereiras ('Packham's Triumph' e 'Kieffer'. As variáveis analisadas foram: diâmetro da secção do tronco no ponto de enxertia, 5 cm abaixo e 5 cm acima do ponto de enxertia, diferença do diâmetro entre porta-enxerto e copa, altura das plantas, volume e massa seca da copa e raízes. Além disso, efetuou-se a observação da conexão vascular no ponto de enxertia através da imersão da base das plantas (abaixo do ponto de enxertia, em solução corante de Ácido Fuccínico 0,08%. Concluiu-se que a cultivar 'Packham's Triumph'apresenta compatibilidade de enxertia com o marmeleiro cultivares 'Adams'e 'Quince C', enquanto o híbrido 'Kieffer' apresentou sintomas morfológicos de incompatibilidade de enxertia com o marmeleiro cultivares 'Quince C' e 'Adams'.The lack of studies on compatibility of pear cultivars and rootstocks is one of the limiting factors on the development of the pear crop in Brazil. The use of quinces as rootstocks for pear cultivars has several advantages, among them the reduction in vigor and earlier bearing trees, however, its combination with some scions cultivars results in problems of incompatibility , such as lost of trees of the orchard due to break of the graft union. The objective of this study was to determine the compatibility between pears cvs. Packham's Triumph and Kieffer

  20. CULTIVAR RELEASE - CD 120 – wheat cultivar, standard quality soft, for the Southern Region of Brazil

    Directory of Open Access Journals (Sweden)

    Ivan Schuster

    2011-01-01

    Full Text Available The cultivar CD 120, developed by the Cooperativa Central de Pesquisa Agrícola (COODETEC is suitable for cultivation in the VCU wheat-producing regions 1 and 2 of the states of Paraná, Santa Catarina and Rio Grande do Sul. It is tolerant to the major wheat diseases and pre-harvest germination and has soft wheat quality and a 6.5 % higher grain yield (3.678 kg ha-1 than the control.