WorldWideScience

Sample records for chemiluminescence

  1. Chemiluminescence multichannel immunosensor

    Energy Technology Data Exchange (ETDEWEB)

    Yacoub-George, E.; Scheithauer, W.; Koppi, A.; Meixner, L.; Drost, S. [Fraunhofer Inst. fuer Mikroelektronische Schaltungen und Systeme (IMS), Muenchen (Germany); Wolf, H. [Regensburg Univ. (Germany). Inst. of Medical Microbiology and Hygiene

    2001-07-01

    An automated chemiluminescence multichannel immunosensor for the parallel detection of three different substances is presented. The system is based on the chemiluminescence capillary ELISA technique in combination with a miniaturised fluidics system. Fused silica capillaries (FSC) are used as support for the immobilized antibodies. The sensing component consists of three stripe photodiodes. An integrated flow-through system ensures automation of the assay cycles. Data on the detection of the bacterial toxin staphylococcal enterotoxin B (SEB), the bacteriophage M13 and Escherichia coli O157:H7 are presented. (orig.)

  2. Chemiluminescence and bioluminescence microbe detection

    Science.gov (United States)

    Taylor, R. E.; Chappelle, E.; Picciolo, G. L.; Jeffers, E. L.; Thomas, R. R.

    1978-01-01

    Automated biosensors for online use with NASA Water Monitoring System employs bioluminescence and chemiluminescence techniques to rapidly measure microbe contamination of water samples. System eliminates standard laboratory procedures requiring time duration of 24 hours or longer.

  3. Chemiluminescence methods (present and future)

    OpenAIRE

    2002-01-01

    This article provides a general review of chemiluminescent methods in some of their recent applications in drug analysis, sea water analysis or antioxidant activity of natural and synthetic products (including olive oil). Practical considerations are not included in the review since the main interest is to state, through the aforementioned applications, that chemiluminescence has been, is, and will be a versatile tool for Analytical Chemistry in future years.Este artículo da una visi&#...

  4. Chemiluminescence methods (present and future

    Directory of Open Access Journals (Sweden)

    Navas, M. J.

    2002-03-01

    Full Text Available This article provides a general review of chemiluminescent methods in some of their recent applications in drug analysis, sea water analysis or antioxidant activity of natural and synthetic products (including olive oil. Practical considerations are not included in the review since the main interest is to state, through the aforementioned applications, that chemiluminescence has been, is, and will be a versatile tool for Analytical Chemistry in future years.Este artículo da una visión general de los métodos de quimiluminiscencia en algunas de las aplicaciones más recientes en análisis de drogas, análisis del agua marina o la actividad antioxidante de productos naturales y de síntesis (incluyendo el aceite de oliva. Las consideraciones prácticas no están incluidas ya que el principal interés es establecer, a través de las aplicaciones mencionadas, que la quimiluminiscencia ha sido, es y será en los próximos años una herramienta versátil de la Química Analítica.

  5. Analytical Applications of Bioluminescence and Chemiluminescence

    Science.gov (United States)

    Chappelle, E. W. (Editor); Picciolo, G. L. (Editor)

    1975-01-01

    Bioluminescence and chemiluminescence studies were used to measure the amount of adenosine triphosphate and therefore the amount of energy available. Firefly luciferase - luciferin enzyme system was emphasized. Photometer designs are also considered.

  6. Native vs photoinduced chemiluminescence in dimethoate determination

    OpenAIRE

    Catalá Icardo, Mónica; López Paz, José Luis; CHOVES BARÓN, CRISTINA; Pena Badena, A.

    2012-01-01

    The determination of dimethoate using either its native chemiluminescent (CL) properties or its photoinduced chemiluminescence obtained by irradiation with a 15W low-pressure mercury lamp was studied. Thereby, two flow injection systems (FIA) with and without irradiation were exhaustively optimized and their analytical characteristics studied. Better sensitivity and selectivity was found in absence of irradiation, due to the enhancing effect of hexadecylpyridinium chloride (HPC), which acted ...

  7. Chemiluminescence of iron-chlorophyllin.

    Science.gov (United States)

    Nagoshi, Toshimasa; Ohno, Osamu; Kotake, Tomohiko; Igarashi, Shukuro

    2005-01-01

    The iron-chlorophyllin complex was found to be chemiluminescent (CL) in an acetonitrile (22%)/water mixed solvent. In the presence of hydrogen peroxide, when iron-chlorophyllin was added to the mixed solvent, a sharp CL signal immediately appeared. Also, analysis of the absorption spectra revealed decomposition of iron-chlorophyllin (based on decrease in absorbance at 396 nm), hence iron-chlorophyllin is the CL substance. Moreover, the CL intensity decreased in the presence of potassium thiocyanate (KSCN), indicating that the axial coordinative position of iron-chlorophyllin acts as a point of catalytic activation. In addition, when fluorophores were present with iron-chlorophyllin CL, their CL intensity values were similar to or greater than that of the well-known trichlorophenylperoxalate ester (TCPO) CL. Thus, during the decomposition reaction of iron-chlorophyllin, the latter transfers its energy to the coexisting fluorophores. Moreover, since the decomposed compound in this CL reaction had a fluorescence, it was found that the iron-chlorophyllin also functions as an energy donor. Therefore, the iron-chlorophyllin complex acts not only as a CL substance, but also as a catalyst and energy donor in the reaction.

  8. Native vs photoinduced chemiluminescence in dimethoate determination

    Energy Technology Data Exchange (ETDEWEB)

    Catala-Icardo, M., E-mail: mocaic@qim.upv.es [Instituto de investigacion para la Gestion Integrada de Zonas Costeras (IGIC), Universidad Politecnica de Valencia, C/Paranimf n Degree-Sign 1, 46730 Grao de Gandia, Valencia (Spain); Lopez-Paz, J.L.; Choves-Baron, C.; Pena-Badena, A. [Instituto de investigacion para la Gestion Integrada de Zonas Costeras (IGIC), Universidad Politecnica de Valencia, C/Paranimf n Degree-Sign 1, 46730 Grao de Gandia, Valencia (Spain)

    2012-01-13

    Highlights: Black-Right-Pointing-Pointer Comparison between native and photoinduced chemiluminescence of dimethoate. Black-Right-Pointing-Pointer Hexadecylpyridinium enhanced greatly the chemiluminescence. Black-Right-Pointing-Pointer Solid phase extraction allowed the successful determination of dimethoate in water. - Abstract: The determination of dimethoate using either its native chemiluminescent (CL) properties or its photoinduced chemiluminescence obtained by irradiation with a 15 W low-pressure mercury lamp was studied. Thereby, two flow injection systems (FIA) with and without irradiation were exhaustively optimized and their analytical characteristics studied. Better sensitivity and selectivity was found in absence of irradiation, due to the enhancing effect of hexadecylpyridinium chloride (HPC), which acted as a sensitizer. In the developed FIA-CL system, the alkaline hydrolysis of dimethoate with NaOH was performed on-line in presence of HPC. The oxidation of the product of hydrolysis with Ce(IV) in hydrochloric medium induced chemiluminescence. The method provided a limit of detection of only 0.05 ng mL{sup -1} without any pre-treatment. However, the combination with solid phase extraction allowed the removal of some potential interferents as well as the preconcentration of the pesticide. Finally, the developed method was successfully applied to natural waters with recoveries between 95 and 108%.

  9. 40 CFR 1065.270 - Chemiluminescent detector.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Chemiluminescent detector. 1065.270 Section 1065.270 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION... with a bypass line if it is needed to facilitate this verification. (d) Humidity effects. You...

  10. Native vs photoinduced chemiluminescence in dimethoate determination.

    Science.gov (United States)

    Catalá-Icardo, M; López-Paz, J L; Choves-Barón, C; Peña-Bádena, A

    2012-01-13

    The determination of dimethoate using either its native chemiluminescent (CL) properties or its photoinduced chemiluminescence obtained by irradiation with a 15 W low-pressure mercury lamp was studied. Thereby, two flow injection systems (FIA) with and without irradiation were exhaustively optimized and their analytical characteristics studied. Better sensitivity and selectivity was found in absence of irradiation, due to the enhancing effect of hexadecylpyridinium chloride (HPC), which acted as a sensitizer. In the developed FIA-CL system, the alkaline hydrolysis of dimethoate with NaOH was performed on-line in presence of HPC. The oxidation of the product of hydrolysis with Ce(IV) in hydrochloric medium induced chemiluminescence. The method provided a limit of detection of only 0.05 ng mL(-1) without any pre-treatment. However, the combination with solid phase extraction allowed the removal of some potential interferents as well as the preconcentration of the pesticide. Finally, the developed method was successfully applied to natural waters with recoveries between 95 and 108%.

  11. Real-time chemiluminescence imaging using nano-lantern probes.

    Science.gov (United States)

    Arai, Yoshiyuki; Nagai, Takeharu

    2014-01-01

    Chemiluminescence imaging can be performed without excitation light sources at various spatial levels ranging from a single cell to the whole body. Thus far, chemiluminescence imaging has been primarily performed with long exposure times because of weak signals, resulting in low temporal resolution. Recently, the brightest-known chemiluminescent proteins--Nano-lantern and Nano-lantern-based functional indicators--have been developed. Nano-lantern probes break the limitation of temporal resolution and enable chemiluminescence imaging of living samples such as cells, plants, and small animals at video rates. This unit describes one protocol for observation of a freely moving unshaved mouse transplanted with Nano-lantern-expressing tumor cells, and another for compatible use of optogenetic tools and a Nano-lantern calcium indicator. Both protocols utilize the synchronization of illumination and camera acquisition sessions, thereby enabling real-time chemiluminescence imaging.

  12. Near-Infrared Intraoperative Chemiluminescence Imaging

    KAUST Repository

    Büchel, Gabriel E.

    2016-08-03

    Intraoperative imaging technologies recently entered the operating room, and their implementation is revolutionizing how physicians plan, monitor, and perform surgical interventions. In this work, we present a novel surgical imaging reporter system: intraoperative chemiluminescence imaging (ICI). To this end, we have leveraged the ability of a chemiluminescent metal complex to generate near-infrared light upon exposure to an aqueous solution of Ce4+ in the presence of reducing tissue or blood components. An optical camera spatially resolves the resulting photon flux. We describe the construction and application of a prototype imaging setup, which achieves a detection limit as low as 6.9pmolcm-2 of the transition-metal-based ICI agent. As a proof of concept, we use ICI for the invivo detection of our transition metal tracer following both systemic and subdermal injections. The very high signal-to-noise ratios make ICI an interesting candidate for the development of new intraoperative imaging technologies. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. [Chemiluminescence spectroscopic analysis of homogeneous charge compression ignition combustion processes].

    Science.gov (United States)

    Liu, Hai-feng; Yao, Ming-fa; Jin, Chao; Zhang, Peng; Li, Zhe-ming; Zheng, Zun-qing

    2010-10-01

    To study the combustion reaction kinetics of homogeneous charge compression ignition (HCCI) under different port injection strategies and intake temperature conditions, the tests were carried out on a modified single-cylinder optical engine using chemiluminescence spectroscopic analysis. The experimental conditions are keeping the fuel mass constant; fueling the n-heptane; controlling speed at 600 r x min(-1) and inlet pressure at 0.1 MPa; controlling inlet temperature at 95 degrees C and 125 degrees C, respectively. The results of chemiluminescence spectrum show that the chemiluminescence is quite faint during low temperature heat release (LTHR), and these bands spectrum originates from formaldehyde (CH2O) chemiluminescence. During the phase of later LTHR-negative temperature coefficient (NTC)-early high temperature heat release (HTHR), these bands spectrum also originates from formaldehyde (CH2O) chemiluminescence. The CO--O* continuum is strong during HTHR, and radicals such as OH, HCO, CH and CH2O appear superimposed on this CO--O* continuum. After the HTHR, the chemiluminescence intensity is quite faint. In comparison to the start of injection (SOI) of -30 degrees ATDC, the chemiluminescence intensity is higher under the SOI = -300 degrees ATDC condition due to the more intense emissions of CO--O* continuum. And more radicals of HCO and OH are formed, which also indicates a more intense combustion reaction. Similarly, more intense CO--O* continuum and more radicals of HCO and OH are emitted under higher intake temperature case.

  14. Determination of ribavirin by flow injection chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    Juqing Jin; Yuhai Tang; Langchong He; Hong Yao; Yuanyuan Sun

    2006-01-01

    Objective: To establish a rapid and precise continuous flow-injection chemiluminescence (CL) method to determine ribavirin. Methods: The ribavirin could restrain strongly CL reaction of luminol in sodium hydroxide (NaOH) solution with potassium chlorate(KClO3). The different experimental parameters affecting the CL intensity were studied carefully. Results:Under optimum conditions(The concentrations of luminol,KClO3, and NaOH were at 0.1 mmol/L,0.5 μmol/L,and 0.2 mol/L, respectively), the linear range of the working curves was 0.01-7.00 μg/ml with a detection limit of 0.004 μg/ml. Conclusion: The method is simple, rapid and sensitive, and successfully applied to the determination of ribavirin in pharmaceutical preparations and biological fluids.

  15. Chemiluminescent solid lipid nanoparticles (SLN) and interations with intact skin

    Science.gov (United States)

    Breidenich, Jennifer; Patrone, Julia; Kelly, Lisa; Benkoski, Jason; Le, Huong; Sample, Jennifer

    2009-08-01

    We report the synthesis and characterization of a novel nanoparticle formulation designed for skin penetration for the purpose of skin imaging. Solid lipid nanoparticles (SLNs), a drug delivery vehicle, were used as the matrix for targeted delivery of peroxide-sensitive chemiluminescent compounds to the epidermis. Luminol and oxalate were chosen as the chemiluminescent test systems, and a formulation was determined based upon non-toxic components, lotion-like properties, and longevity/visibility of a chemiluminescent signal. The luminescence lifetime was extended in the lipid formulation in comparison to the chemiluminescent system in solution. When applied to porcine skin, our formulation remained detectable relative to negative and positive controls. Initial MTT toxicity testing using HepG2 cells have indicated that this formulation is relatively non-toxic. This formulation could be used to image native peroxides present in tissue that may be indicative of skin disease.

  16. A new modular chemiluminescence immunoassay analyser evaluated.

    Science.gov (United States)

    Ognibene, A; Drake, C J; Jeng, K Y; Pascucci, T E; Hsu, S; Luceri, F; Messeri, G

    2000-03-01

    Thyrotropin (TSH), free thyroxine (fT4) and testosterone assays have been used as a probe to evaluate the performances of a new modular chemiluminescence (CL) immunoassay analyser, the Abbott Architect 2000. The evaluation was run in parallel on other systems that use CL as the detection reaction: DPC Immulite, Chiron Diagnostics ACS-180 and ACS Centaur (TSH functional sensitivity only). TSH functional sensitivity was 0.0012, 0.009, 0.033 and 0.039 mU/I for the Architect, Immulite, ACS Centaur and ACS-180, respectively. Testosterone functional sensitivity was 0.38, 3.7 and 2.0 nmol/l for Architect, Immulite and ACS-180, respectively. Good correlation was obtained between the ACS-180 and Architect for all assays. The Immulite correlation did not agree well with the Architect or ACS-180 for fT4 and testosterone but was in good agreement for TSH. Regarding fT4 and testosterone, equilibrium dialysis and isotopic dilution gas-chromatography mass-spectrometry (GC-MS) respectively were used as reference methods. For both within- and between-run precision, the Architect showed the best reproducibility for all three analytes (CV < 6%).

  17. Quantifying acoustic damping using flame chemiluminescence

    Science.gov (United States)

    Boujo, E.; Denisov, A.; Schuermans, B.; Noiray, N.

    2016-12-01

    Thermoacoustic instabilities in gas turbines and aeroengine combustors falls within the category of complex systems. They can be described phenomenologically using nonlinear stochastic differential equations, which constitute the grounds for output-only model-based system identification. It has been shown recently that one can extract the governing parameters of the instabilities, namely the linear growth rate and the nonlinear component of the thermoacoustic feedback, using dynamic pressure time series only. This is highly relevant for practical systems, which cannot be actively controlled due to a lack of cost-effective actuators. The thermoacoustic stability is given by the linear growth rate, which results from the combination of the acoustic damping and the coherent feedback from the flame. In this paper, it is shown that it is possible to quantify the acoustic damping of the system, and thus to separate its contribution to the linear growth rate from the one of the flame. This is achieved by post-processing in a simple way simultaneously acquired chemiluminescence and acoustic pressure data. It provides an additional approach to further unravel from observed time series the key mechanisms governing the system dynamics. This straightforward method is illustrated here using experimental data from a combustion chamber operated at several linearly stable and unstable operating conditions.

  18. Luminol-potassium permanganate chemiluminescence system for the determination of three anthracycline antibiotics

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Objective To establish a flow-injection chemiluminescence method for the determination of doxorubicin,epirubicin and mitoxantrone and study its reaction mechanism.Methods In alkaline medium,chemiluminescence of luminol-potassium permanganate system could be inhibited obviously by anthracycline antibiotics.Combined with flow-injection technique,a new chemiluminescence method for determining the anthracycline antibiotics was set up.The chemiluminescence mechanism of the luminol-potassium permanganate system w...

  19. Ultraweak chemiluminescence of rice seeds during accelerated aging

    Science.gov (United States)

    Chen, Wenli; Xing, Da; He, Yonghong

    2002-04-01

    Ultraweak Chemiluminescence (UCL) studies of different aging degree of rice (Oryza sativa L.) seeds stored in a high temperature 40 degree(s)C and high relative humidity 90% environment (0 day, 8 days, 15 days, and 22 days) were carried out. We firstly observed that aging degree of rice seeds was positive correlation with ultraweak chemiluminescence during the early imbibition (0-1h). Addition of water to rice seeds stimulates ultraweak chemiluminescence, the intensity of which depends upon aging degree of seeds. The shorter the seed accelerated aging time was, the higher the intensity of the UCL in the early imbibition period, the lower hydrogen peroxide (H2O2) concentration of rice seeds, the higher percentage seed germination. The germination and superoxide dismutase (SOD) activity of dry rice seeds was obvious positive correlation with the intensity of UCL. While catalase (CAT) activity of rice seeds was determined. Mechanism of ultraweak chemiluminescence was discussed. It was concluded that the store time of rice seeds could be judged from their UCL characters during the early imbibition period, which might be a way to examine vigor of seeds.

  20. Tandem chemiluminescence-flow injection analysis for dimethoate determination.

    OpenAIRE

    Catalá Icardo, Mónica; López Paz, José Luis; CHOVES BARÓN, CRISTINA

    2010-01-01

    Ministry of Education and Science [CTM2006-11991] FEDER funds Catalá Icardo, M.; López Paz, JL.; Choves Barón, C. (2010). Tandem chemiluminescence-flow injection analysis for dimethoate determination. Luminescence. 25:235-236. doi:10.1002/bio.1217. Senia 235 236 25

  1. Chemiluminescent reductive acridinium triggering (CRAT)-mechanism and applications

    NARCIS (Netherlands)

    Zomer, Bert; Colle, Loes; Jedynska, Aleksandra; Pasterkamp, Gerard; Kooter, Ingeborg; Bloemen, Henk

    2011-01-01

    Acridinium esters traditionally are triggered using basic hydrogen peroxide. By serendipity, we have found that acridinium esters can also be triggered with emission of chemiluminescence by reductive triggering, e.g., by zinc metal or reduced forms of ferric and cupric salts. Furthermore, organic re

  2. Field-flow fractionation of cells with chemiluminescence detection.

    Science.gov (United States)

    Melucci, Dora; Roda, Barbara; Zattoni, Andrea; Casolari, Sonia; Reschiglian, Pierluigi; Roda, Aldo

    2004-11-12

    Field-flow fractionation is a separation technique characterized by a retention mechanism which makes it suitable for sorting cells over a short analysis time, with low sample carry-over and preserving cell viability. Thanks to its high sensitivity, chemiluminescence detection is suitable for the quantification of just a few cells expressing chemiluminescence or bioluminescence. In this work, different formats for coupling gravitational field-flow fractionation and chemiluminescence detection are explored to achieve ultra-sensitive cell detection in the framework of cell sorting. The study is carried out using human red blood cells as model sample. The best performance is obtained with the on-line coupling format, performed in post-column flow-injection mode. Red cells are isolated from diluted whole human blood in just a few minutes and detected using the liquid phase chemiluminescent reaction of luminol catalysed by the red blood cell heme. The limit of detection is a few hundred injected cells. This is lower than the limit of detection usually achieved by means of conventional colorimetric/turbidimetric methods, and it corresponds to a red blood cell concentration in the injected sample of five orders of magnitude lower than in whole blood.

  3. Investigation of Chemiluminescence with Electrogenerated Reagents and Its Analytical Application

    Institute of Scientific and Technical Information of China (English)

    章竹君; 李保新; 郑行望

    2003-01-01

    In this paper, studies on chemiluminescence (CL) systems with electrogenerated reagents, including BrO-, ClO-, Br2, [Cu-(HIO6)2]5-, H2O2, Mn3+, Co3+ and Ag2+, are described.The analytical applications of the CL system with electrogenerated reagents are reviewed.

  4. Chemiluminescent ozone, NO, and NO/sub 2/ detectors

    Energy Technology Data Exchange (ETDEWEB)

    van Heusden, S.

    1974-07-05

    Autonomous apparatuses for the measurement of the ozone, nitric oxide and nitrogen dioxide concentrations in the air by chemiluminescent technique, developed for use in the Dutch air quality monitoring network, are described. One ozone detector, using ethene for the chemiluminescent reaction of ozone, has a maximum at 420 nanom, and has a detection range from 2 ppB to 1,000 ppM. The error amounts to 3%, and the time required for measurement can be reduced to 0.1 sec. Another ozone detector uses gallic acid with Rhodamine B on silica gel for the chemiluminescent reaction with an emission maximum at 560 nanom. This detector can be used for a concentration range of 0.1 ppB to 400 ppB. Normal concentrations of sulfur dioxide, nitric oxide, nitrogen dioxide, methane, carbon monoxide, and carbon dioxide do not interfere with the determination. A chemiluminescent detector for the joint determination of NO and NO/sub 2/ oxidizes NO to NO/sub 2/ by means of ozone, while the NO/sub 2/ originally present in the air is reduced to NO for the determination of the total nitrogen oxide content at an emission peak at 1200 nanom. No interference due to SO/sub 2/, CH/sub 4/, O/sub 3/, CO, or CO/sub 2/ was observed. The range of measurement extends from 2 ppB to 50 ppM. Another chemiluminescent analyzer for ozone, NO, and NO/sub 2/ uses gallic acid and Rhodamine B for the determination of ozone, while NO/sub 2/ is decomposed to NO and native oxygen and further to O/sub 3/ by photolysis under ultraviolet irradiation, and NO is oxidized to NO/sub 2/ by a solid reagent for subsequent reaction to ozone. The limits of detectability are 0.5 ppB for nitrogen oxides and 0.1 ppB for ozone, the upper limit being 150 ppB. All these chemiluminescent detectors comprise photomultipliers and are suitable for autonomous operation for 2 to 3 mo.

  5. A novel chemiluminescence method for the determination of ergometrine maleate in serum sample without chemiluminescence reagent.

    Science.gov (United States)

    Hu, Yufei; Zhang, Zhujun; Li, Gongke

    2010-04-15

    In this paper, a novel flow injection-chemiluminescence (FI-CL) method was proposed for the determination of ergometrine maleate in serum. The new CL reaction was based on the direct oxidation of ergometrine maleate by the complex of metal chelate diperiodatocuprate(III) (K(5)[Cu(HIO(6))(2)]) in an alkaline medium. The CL intensity was enhanced in the presence of ascorbic acid. Hereby under the optimum conditions, ergometrine maleate was determined over the range of 4.0 x 10(-9) gm L(-1) to 4.0 x 10(-7) gm L(-1) with a limit of detection (3 sigma) of 1.1 x 10(-9) gm L(-1). The relative standard deviation (R.S.D.) was 2.1% for 8.0 x 10(-9) gm L(-1) ergometrine maleate (n=7). The sensitive method was successfully applied to the direct determination of ergometrine maleate (ng mL(-1)) in pharmaceutical injection and serum samples. The mechanism of the reactions was also discussed.

  6. Chemiluminescence assay of lipase activity using a synthetic substrate as proenhancer for luminol chemiluminescence reaction.

    Science.gov (United States)

    Ichibangase, Tomoko; Ohba, Yoshihito; Kishikawa, Naoya; Nakashima, Kenichiro; Kuroda, Naotaka

    2004-01-01

    A novel chemiluminescence (CL) assay method for lipase (triacylglycerol lipase, E.C.3.1.1.3) activity was developed by using the lauric acid ester of 2-(4-hydroxyphenyl)-4,5-diphenylimidazole (HDI) as a substrate. The method is based on the enhanced CL reaction of luminol-hydrogen peroxide-horseradish peroxidase (HRP) with HDI that is liberated from the substrate by enzymatic hydrolysis. To simplify the assay procedure, both the hydrolysis of the substrate and the enhanced CL reaction were performed in the same reaction mixture. Lipases from Candida cylindracea and porcine pancreas were successfully determined with the detection limits (blank signal + 3 SD) of 0.05 and 50.0 mU/tube, respectively. The method is simple and rapid, permitting the completion of single assay within 5 min. The reproducibilities obtained with replicate assays were relative standard deviations (RSDs) of 4.7% for within-day and 6.0% for between-day assays.

  7. Detection of gamma irradiated pepper and papain by chemiluminescence

    Science.gov (United States)

    Sattar, Abdus; Delincée, H.; Diehl, J. F.

    Chemiluminescence (CL) measurements of black pepper and of papain using luminol and lucigenin reactions were studied. Effects of grinding, irradiation (5-20 kGy) and particle size (750-140 μm) on CL of pepper, and of irradiation (10-30 kGy) on CL of papain, were investigated. All the tested treatments affected the luminescence response in both the luminol and lucigenin reactions; however, the pattern of changes in each case, was inconsistent. Optimum pepper size for maximum luminescence was 560 μm, and optimum irradiation doses were >15 kGy for pepper and >20 kGy for papain. Chemiluminescence may possibly be used as an indicator or irradiation treatment for pepper and papain at a dose of 10 kGy or higher, but further research is needed to establish the reliability of this method.

  8. Chemiluminescent Detection of Enzymatically Produced H2S

    OpenAIRE

    Bailey, T. Spencer; Pluth, Michael D.

    2015-01-01

    Hydrogen sulfide (H2S) has emerged as an important biological signaling molecule. To better understand the multifaceted biological roles of H2S, the development of selective and sensitive biocompatible assays for H2S is becoming increasingly important. Motivated by these challenges, our laboratory is developing new methods to further detect and monitor biological H2S. Here, we describe in detail our recent advances in the development and the use of chemiluminescence-based H2S sensors to assis...

  9. [Decrease of singlet oxygen chemiluminescence by the presence of carnosine].

    Science.gov (United States)

    Svachko, A G; Formaziuk, V E; Sergienko, V I

    1990-08-01

    To test antiradical medicines effect the chemical production of singlet oxygen (NaClO + H2O2) was investigated. The quantity singlet oxygen chemiluminescence was decreased in the presence of Japanese Catalin and Chine Baineiting, antirheumatic Voltaren and less strong Finish Catachrome and Carnosine. American Quinax does not possess such an effect. One of the possible starting mechanisms causing different diseases (atherosclerosis, cataract etc.) is destruction of biomembranes by active forms of oxygen.

  10. Investigation on the spontaneous combustion of refuse-derived fuels during storage using a chemiluminescence technique.

    Science.gov (United States)

    Matunaga, Atsushi; Yasuhara, Akio; Shimizu, Yoshitada; Wakakura, Masahide; Shibamoto, Takayuki

    2008-12-01

    Refuse-derived fuel (RDF), a high-caloric material, is used by various combustion processes, such as power plants, as alternative fuel. Several explosion accidents, however, possibly initiated by the spontaneous combustion of stored RDF, have been reported in Japan. Therefore the spontaneous combustion of RDF prepared from domestic garbage was investigated using chemiluminescence. RDF samples were heated either under air or under nitrogen for 1, 2, or 4 h at 120 or 140 degrees C and then cooled by an air or nitrogen stream. All RDF samples exhibited chemiluminescence. In air-treated RDF samples (heated and cooled by air), chemiluminescence after ageing was shown to be slightly lower than before ageing, whereas in nitrogen-treated samples (both heated and cooled by nitrogen) chemiluminescence decreased significantly after ageing. When nitrogen was replaced with air during aging, however, a sudden increase of chemiluminescence was observed. On the other hand, when cooling was done with air, chemiluminescence increased. Higher chemiluminescence was also observed during high-temperature treatment. Further experiments on cellulose, one of the major components of domestic garbage, exhibited similar chemiluminescence patterns to those of RDF when treated by the same methods as those used for RDF ageing. Chemiluminescence from cellulose increased significantly when the atmospheric gas was changed from nitrogen to air, suggesting that oxygen in the air promoted the formation of hydroperoxide from cellulose. Therefore, it is hypothesized that cellulose plays an important role in the formation of chemiluminescence from RDF. The formation of chemiluminescence indicated that radicals are formed from RDF by oxidation or thermal degradation at room or atmospheric temperatures and may subsequently lead to spontaneous combustion.

  11. Effect of Saliva on Measurement of Chemiluminescence by a Micro-Reactor Incorporating a Micro-Channel

    OpenAIRE

    Tsukagoshi, Kazuhiko; Fukumoto, Kazuaki; Nakajima, Riichiro; Yamashita, Kenichi; Maeda, Hideaki

    2007-01-01

    Effect of saliva on measurement of chemiluminescence was examined by a micro-reactor incorporating a micro-channel. Sodium hypochlorite and hydrogen peroxide solutions were delivered into a micro-channel developed in a micro-reactor by a syringe pump, providing a laminar flow liquid-liquid interface in the channel and leading to chemiluminescence from singlet oxygen. It was found under certain conditions including saliva that ca. 5% chemiluminescence of the total chemiluminescence was lost in...

  12. Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex

    Science.gov (United States)

    Matsunaga, Tadashi; Sato, Rika; Kamiya, Shinji; Tanaka, Tsuyosi; Takeyama, Haruko

    1999-04-01

    Bacterial magnetic particles (BMPs) which have ProteinA expressed on their surface were constructed using magA which is a key gene in BMP biosynthesis in the magnetic bacterium Magnetospirillum sp. AMB-1. Homogenous chemiluminescence enzyme immunoassay using antibody bound ProteinA-BMP complexes was developed for detection of human IgG. A good correlation between the luminescence yield and the concentration of human IgG was obtained in the range of 1-10 3 ng/ml.

  13. Synthesis and properties of differently charged chemiluminescent acridinium ester labels.

    Science.gov (United States)

    Natrajan, Anand; Sharpe, David

    2013-02-14

    Chemiluminescent acridinium dimethylphenyl esters containing N-sulfopropyl groups in the acridinium ring are highly sensitive, hydrophilic labels that are used in automated immunoassays for clinical diagnostics. Light emission from these labels is triggered with alkaline peroxide in the presence of a cationic surfactant. At physiological pH, N-sulfopropyl acridinium esters exist as water adducts that are commonly referred to as pseudobases. Pseudobase formation, which results from addition of water to the zwitterionic N-sulfopropyl acridinium ring, neutralizes the positive charge on the acridinium nitrogen and imparts a net negative charge to the label due to the sulfonate moiety. As a consequence, N-sulfopropyl acridinium ester conjugates of small molecule haptens as well as large molecules such as proteins gain negative charges at neutral pH. In the current study, we describe the synthesis and properties of two new hydrophilic acridinium dimethylphenyl ester labels where the net charge in the labels was altered. In one label, the structure of the hydrophilic N-alkyl group attached to the acridinium ring was changed so that the pseudobase of the label contains no net charge. In the second acridinium ester, two additional negative charges in the form of sulfopropyl groups were added to the acridinium ring to make this label's pseudobase strongly anionic. Chemiluminescence measurements of these labels, as well as their conjugates of an antibody with a neutral pI, indicate that acridinium ester charge while having a modest effect on emission kinetics has little influence on light output. However, our results demonstrate that acridinium ester charge can affect protein pI, apparent chemiluminescence stability and non-specific binding of protein conjugates to microparticles. These results emphasize the need for careful consideration of acridinium ester charge in order to optimize reagent stability and performance in immunoassays. In the current study, we observed that

  14. Stimulation of chemiluminescence by synthetic muramyl dipeptide and analogs.

    OpenAIRE

    Masihi, K N; Azuma, I; Brehmer, W; Lange, W.

    1983-01-01

    The effect on respiratory burst of murine splenic cells after in vitro exposure to synthetic muramyl dipeptide (MDP) and 6-O-acyl and quinonyl derivatives was studied at an early phase of interaction by luminol-dependent chemiluminescence (CL) in response to stimulation by zymosan. The MDP molecule enhanced CL, but the degree of CL response varied with the kinds of fatty acids introduced in the chemical structure of synthetic glycopeptide analogs. A 6-O-acyl derivative possessing an alpha-bra...

  15. Highly sensitive flow-injection chemiluminescence determination of pyrogallol compounds

    Science.gov (United States)

    Kanwal, Shamsa; Fu, Xiaohong; Su, Xingguang

    2009-12-01

    A highly sensitive flow-injection chemiluminescent method for the direct determination of pyrogallol compounds has been developed. Proposed method is based on the enhanced effect of pyrogallol compounds on the chemiluminescence signals of KMnO 4-H 2O 2 system in slightly alkaline medium. Three important pyrogallol compounds, pyrogallic acid, gallic acid and tannic acid, have been detected by this method, and the possible mechanism of the CL reaction is also discussed. The proposed method is simple, convenient, rapid (60 samples h -1), and sensitive, has a linear range of 8 × 10 -10 mol L -1 to 1 × 10 -5 mol L -1, for pyrogallic acid, with a detection limit of 6 × 10 -11 mol L -1, 4 × 10 -8 mol L -1 to 5 × 10 -3 mol L -1 for gallic acid with a detection limit of 9 × 10 -10 mol L -1, and 8 × 10 -8 mol L -1 to 5 × 10 -2 mol L -1 for tannic acid, with a detection limit of 2 × 10 -9 mol L -1, respectively. The relative standard deviation (RSD, n = 15) was 0.8, 1.1 and 1.3% for 5 × 10 -6 mol L -1 pyrogallic acid, gallic acid and tannic acid, respectively. The proposed method was successfully applied to the determination of pyrogallol compounds in tea and coffee samples.

  16. Chemiluminescence assay for the detection of biological warfare agents

    Energy Technology Data Exchange (ETDEWEB)

    Langry, K; Horn, J

    1999-11-05

    A chemiluminescent homogeneous immunoassay and a hand-size multiassay reader are described that could be used for detecting biological materials. The special feature of the assay is that it employs two different antibodies that each bind to a unique epitope on the same antigen. Each group of epitope-specific antibodies has linked to it an enzyme of a proximal-enzyme pair. One enzyme of the pair utilizes a substrate in high concentration to produce a second substrate required by the second enzyme. This new substrate enables the second enzyme to function. The reaction of the second enzyme is configured to produce light. This chemiluminescence is detected with a charge-coupled device (CCD) camera. The proximal pair enzymes must be in close proximity to one another to allow the second enzyme to react with the product of the first enzyme. This only occurs when the enzyme-linked antibodies are attached to the antigen, whether antigen is a single protein with multiple epitopes or the surface of a cell with a variety of different antigens. As a result of their juxtaposition, the enzymes produce light only in the presence of the biological material. A brief description is given as to how this assay could be utilized in a personal bio-agent detector system.

  17. Selective Detection of Neurotransmitters by Fluorescence and Chemiluminescence Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Ziqiang Wang; Edward S. Yeung

    2001-08-06

    In recent years, luminescence imaging has been widely employed in neurochemical analysis. It has a number of advantages for the study of neuronal and other biological cells: (1) a particular molecular species or cellular constituent can be selectively visualized in the presence of a large excess of other species in a heterogeneous environment; (2) low concentration detection limits can be achieved because of the inherent sensitivity associated with fluorescence and chemiluminescence; (3) low excitation intensities can be used so that long-term observation can be realized while the viability of the specimen is preserved; and (4) excellent spatial resolution can be obtained with the light microscope so subcellular compartments can be identified. With good sensitivity, temporal and spatial resolution, the flux of ions and molecules and the distribution and dynamics of intracellular species can be measured in real time with specific luminescence probes, substrates, or with native fluorescence. A noninvasive detection scheme based on glutamate dehydrogenase (GDH) enzymatic assay combined with microscopy was developed to measure the glutamate release in cultured cells from the central nervous system (CNS). The enzyme reaction is very specific and sensitive. The detection limit with CCD imaging is down to {micro}M levels of glutamate with reasonable response time. They also found that chemiluminescence associated with the ATP-dependent reaction between luciferase and luciferin can be used to image ATP at levels down to 10 nM in the millisecond time scale. Similar imaging experiments should be feasible in a broad spectrum of biological systems.

  18. Gold nanoparticles-based chemiluminescence resonance energy transfer for ultrasensitive detection of melamine.

    Science.gov (United States)

    Du, Jianxiu; Wang, Yadi; Zhang, Weimin

    2015-01-01

    A turn-on chemiluminescence resonance energy transfer method was fabricated for the determination of melamine by using bis(2,4,6-trichlorophenyl)oxalate-hydrogen peroxide-fluorescein chemiluminescence reaction as a donor and dispersed gold nanoparticles as an acceptor. The chemiluminescence signal of bis(2,4,6-trichlorophenyl)oxalate-hydrogen peroxide-fluorescein reaction decreased significantly in the presence of dispersed gold nanoparticles because the absorption band of dispersed gold nanoparticles perfectly overlapped with the chemiluminescence spectrum. Melamine could induce the aggregation of gold nanoparticles, leading to a dramatic red-shift of the absorption band of dispersed gold nanoparticles. The absorption band of the aggregated gold nanoparticles does not overlap with the chemiluminescence spectrum of the reaction. In such a case, chemiluminescence resonance energy transfer could not happen and the chemiluminescence signal was restored. The procedure allowed the measurement of 3.2×10(-12)-3.2×10(-7) mol/L melamine with a limit of detection of 3×10(-13) mol/L. The method was applied to the determination of melamine in spiked milk samples; with recoveries within the range 94.1-104.2%.

  19. Review of Chemiluminescence as an Optical Diagnostic Tool in High Pressure Unstable Rockets

    Science.gov (United States)

    Fuller, Tristan L.

    The purpose of this project was to investigate the effects of optical absorption on line-of-sight integrated chemiluminescence measurements in high pressure rockets. The use of chemiluminescent emissions has been used in the past in an effort to characterise the flame reaction zone and the corresponding heat release, however most efforts have been with low pressure or atmospheric flames. Chemiluminescent measurements have been used in the case of the Continuously Variable Resonant Combustor (CVRC) in an attempt to validate a CFD simulation of the same system, although the CVRC operates at a higher pressure. For higher pressure flames it is unclear if such measurements are valid. To bridge the gap between the experimental and numerical data a spectroscopic model was created to study the validity of chemiluminescent based measurements in the CVRC. It was found that the CVRC combustion medium is optically opaque for the chemiluminescent emissions produced by OH* and optically transparent for the chemiluminescent emissions produced by CH*. Unfortunately, the emissions produced by CH* are largely influenced by the emissions produced by CO 2*. As such, both OH* and CH* are poor indicators of the heat release in the CVRC and therefore chemiluminescence measurements are not useful in validating the CVRC CFD simulation.

  20. [Effects of periodontitis and opsonized zymosan on chemiluminescence of blood neutrophils].

    Science.gov (United States)

    Zekonis, Gediminas; Ivanauskiene, Egle

    2002-01-01

    The objective of the present investigation was to explore oxidative function of parodontitis patient's blood neutrophils stimulated with opsonized zymosan by method of luminol- and lucigenin-dependent chemiluminescence. The leukocytes for this study were obtained from peripheral venous blood of 16 parodontitis patients and 10 healthy subjects. The luminol-dependent chemiluminescence of stimulated neutrophils of parodontitis patients did not differ (p > 0.05) from control subjects (66,849 +/- 6372 cpm and 61,243 +/- 5240 cpm, respectively). Lucigenin-dependent chemiluminescence of stimulated neutrophils of parodontitis patients was increased (p parodontitis patients was significantly higher (p parodontitis patients possibly can affect parodontal health.

  1. Flow-injection-enhanced chemiluminescence method for the determination of three β-blockers

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Objective To develop a rapid,simple and sensitive chemiluminescence method for the determination of three β-blockers (bisoprolol,atenolol and propranolol). Methods The chemiluminescence of cerium (Ⅳ)-sulfite system was obviously sensitized by adding anyone of three β-blockers in acid media. A new chemiluminescence method was set up by combining with flow-injection technique and used to determine the three β-blockers. Results Good linear ranges were obtained at the concentrations of 2.0×10-7g/mL-4.0×10-5g/mL...

  2. 3D-printed and CNC milled flow-cells for chemiluminescence detection.

    Science.gov (United States)

    Spilstead, Kara B; Learey, Jessica J; Doeven, Egan H; Barbante, Gregory J; Mohr, Stephan; Barnett, Neil W; Terry, Jessica M; Hall, Robynne M; Francis, Paul S

    2014-08-01

    Herein we explore modern fabrication techniques for the development of chemiluminescence detection flow-cells with features not attainable using the traditional coiled tubing approach. This includes the first 3D-printed chemiluminescence flow-cells, and a milled flow-cell designed to split the analyte stream into two separate detection zones within the same polymer chip. The flow-cells are compared to conventional detection systems using flow injection analysis (FIA) and high performance liquid chromatography (HPLC), with the fast chemiluminescence reactions of an acidic potassium permanganate reagent with morphine and a series of adrenergic phenolic amines.

  3. Chemiluminescent Detection of Enzymatically Produced H2S

    Science.gov (United States)

    Bailey, T. Spencer; Pluth, Michael D.

    2015-01-01

    Hydrogen sulfide (H2S) has emerged as an important biological signaling molecule. To better understand the multifaceted biological roles of H2S, the development of selective and sensitive biocompatible assays for H2S is becoming increasingly important. Motivated by these challenges, our laboratory is developing new methods to further detect and monitor biological H2S. Here, we describe in detail our recent advances in the development and the use of chemiluminescence-based H2S sensors to assist other investigators with use of these chemical tools. We highlight the use of these tools use by displaying their selectivity and high sensitivity toward H2S and provide examples of assays we have developed to detect enzymatically produced H2S. PMID:25725517

  4. Monocyte chemiluminescence and macrophage precursors in the aged.

    Directory of Open Access Journals (Sweden)

    Takahashi,Isao

    1985-12-01

    Full Text Available Age-related alterations in the host defense system have been vigorously investigated because of increased susceptibility to infection and neoplasms in the aged. Although monocyte-macrophages form a major part of the cellular defense against microorganisms, the majority of investigations has been limited to neutrophils and lymphocytes. The present study, designed to determine the influence of age on mononuclear phagocytes, revealed no significant decrease in the absolute number of blood monocytes, but did reveal a tendency for the chemiluminescence of blood monocytes to decrease (p less than 0.10 and a significant decrease in the numbers of macrophage precursors (p less than 0.05 in the aged (over 70 year old, in comparison with controls (under 40 years old. On the basis of these findings, functional alterations of monocyte-macrophages seem to participate in the increased susceptibility to infection in the aged.

  5. Chemiluminescent nitrogen detection (CLND) to measure kinetic aqueous solubility.

    Science.gov (United States)

    Kestranek, Aimee; Chervenak, Andrew; Longenberger, Justin; Placko, Steven

    2013-01-01

    Solubility is the dose-limiting property for in vitro studies, and therefore is a critical physicochemical property to measure in drug discovery. Solubility data can be used to guide lead optimization, troubleshoot erratic bioassay results, and identify potential downstream liabilities such as insufficient solubility for bioassays or oral bioavailability. Typically, early in vitro studies are performed using library compounds prepared as dimethylsulfoxide (DMSO) stock solutions, resulting in in vitro test solutions containing DMSO at low concentration (<5% v/v). Since DMSO can affect the apparent solubility, it is desirable to obtain solubility data under conditions mimicking the in vitro study. Kinetic solubility (from DMSO stock solutions) is often preferred over thermodynamic solubility (from dry powder) in early drug discovery. The protocols in this article describe a general procedure for assessing kinetic aqueous solubility of early drug discovery compounds using a miniaturized shake flask method with chemiluminescent nitrogen detection (CLND).

  6. Determination of theophylline concentration in serum by chemiluminescent immunoassay

    Institute of Scientific and Technical Information of China (English)

    ZHOU Mei-xia; GUAN Cha-ying; CHEN Guang; XIE Xin-you; WU Sheng-hai

    2005-01-01

    Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum. Methods: To measure the concentration of theophylline (n=122) and evaluate the assay.Results: The linear range of the CLIA method was 0.51~40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 μmol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L). Conclusion: This method is simple, convenient and precise for clinical pharmacokinetics study oftheophylline.

  7. Ester oxidation on an aluminum surface using chemiluminescence

    Science.gov (United States)

    Jones, William R., Jr.; Meador, Michael A.; Morales, Wilfredo

    1986-01-01

    The oxidation characteristics of a pure ester (trimethyolpropane triheptanoate) were studied by using a chemiluminescence technique. Tests were run in a thin film microoxidation apparatus with an aluminum alloy catalyst. Conditions included a pure oxygen atmosphere and a temperature range of 176 to 206 C. Results indicated that oxidation of the ester (containing .001 M diphenylanthracene as an intensifier) was accompanied by emission of light. The maximum intensity of light emission was a function of the amount of ester, the concentration of intensifier, and the test temperature. The induction period, or the time to reach one-half of maximum intensity was inversely proportional to test temperature. Decreases in light emission at the later stages of a test were caused by depletion of the intensifier.

  8. Stimulation of chemiluminescence by synthetic muramyl dipeptide and analogs.

    Science.gov (United States)

    Masihi, K N; Azuma, I; Brehmer, W; Lange, W

    1983-04-01

    The effect on respiratory burst of murine splenic cells after in vitro exposure to synthetic muramyl dipeptide (MDP) and 6-O-acyl and quinonyl derivatives was studied at an early phase of interaction by luminol-dependent chemiluminescence (CL) in response to stimulation by zymosan. The MDP molecule enhanced CL, but the degree of CL response varied with the kinds of fatty acids introduced in the chemical structure of synthetic glycopeptide analogs. A 6-O-acyl derivative possessing an alpha-branched fatty acid chain, B30-MDP, stimulated maximum levels of CL activity. High CL responses were obtained with L8-MDP having a short chain of linear fatty acids and with QS-10-MDP-66 containing a ubiquinone compound. CL was also stimulated by MDP and its analogs in the spleen cells of nude mice lacking mature T lymphocytes, but the extent of stimulation was decreased compared with that of normal spleen cells.

  9. Selective chlorine dioxide determination using gas-diffusion flow injection analysis with chemiluminescent detection

    Energy Technology Data Exchange (ETDEWEB)

    Hollowell, D.A.; Gord, J.R.; Gordon, G.; Pacey, G.E.

    1986-06-01

    An automated chemiluminescent technique has been developed utilizing the advantages of gas-diffusion flow injection analysis. A gas-diffusion membrane separates the donor (sampling) stream from the acceptor (detecting) stream and removes ionic interferences. A novel chemiluminescence flow-through detector cell is used to measure the concentration of chlorine dioxide as a function of the intensity of the chemiluminescence produced from its reaction with luminol. The chemiluminescent reagent merges with the analyte directly in front of the photomultiplier tube in order to maximize the sensitivity of the system. The detection limit for chlorine dioxide is approximately 5 ppb. The method is over 1500 times more selective for chlorine dioxide than for chlorine on a mole basis. This method eliminates interference from iron and manganese compounds, as well as other oxychlorinated compounds such as chlorite ion and chlorate ion.

  10. Surface plasmon coupled chemiluminescence during adsorption of oxygen on magnesium surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Hagemann, Ulrich [Interdisciplinary Center for the Analytics on the Nanoscale (ICAN) and Center for Nanointegration Duisburg-Essen (CENIDE), Carl-Benz-Str. 199, 47057 Duisburg (Germany); Nienhaus, Hermann, E-mail: hermann.nienhaus@uni-due.de [Faculty of Physics, University of Duisburg-Essen and Center for Nanointegration Duisburg-Essen (CENIDE), Lotharstr. 1, 47048 Duisburg (Germany)

    2015-12-28

    The dissociative adsorption of oxygen molecules on magnesium surfaces represents a non-adiabatic reaction exhibiting exoelectron emission, chemicurrent generation, and weak chemiluminescence. Using thin film Mg/Ag/p-Si(111) Schottky diodes with 1 nm Mg on a 10-60 nm thick Ag layer as 2π-photodetectors, the chemiluminescence is internally detected with a much larger efficiency than external methods. The chemically induced photoyield shows a maximum for a Ag film thickness of 45 nm. The enhancement is explained by surface plasmon coupled chemiluminescence, i.e., surface plasmon polaritons are effectively excited in the Ag layer by the oxidation reaction and decay radiatively leading to the observed photocurrent. Model calculations of the maximum absorption in attenuated total reflection geometry support the interpretation. The study demonstrates the extreme sensitivity and the practical usage of internal detection schemes for investigating surface chemiluminescence.

  11. A highly-sensitive multisubstrate-compatible chemiluminescent immunoassay for human fetuin A

    OpenAIRE

    sprotocols

    2015-01-01

    Authors: Sandeep Kumar Vashist ### Abstract We report a highly-sensitive chemiluminescent immunoassay (CIA) for the detection of human fetuin A (HFA), which is based on the leach-proof covalent crosslinking of anti-HFA capture antibodies on 3-aminopropyltriethoxysilane (APTES)-functionalized 96-well chemiluminescent microtiter plates (CMTP) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysulfosuccinimide. It has more than 3-fold reduced overall assay du...

  12. Efficient Generation of Chemiluminescence during the reduction of manganese(IV) ions with lactic acid

    Science.gov (United States)

    Tsaplev, Yu. B.

    2016-12-01

    The kinetics and mechanism of chemiluminescence during the reduction of manganese(IV) ions with lactic acid in an H2SO4-AcOH medium are studied. Kinetic spectrophotometric measurements are used to determine the profiles of change in the concentrations of Mn(IV) and Mn(III) ions during the reaction. The results from kinetic spectrophotometric measurements are compared to the light yield kinetics. The quantum chemiluminescence and chemiexcitation yields reach record values.

  13. Optical tomography of fluorophores in dense scattering media based on ultrasound-enhanced chemiluminescence

    Energy Technology Data Exchange (ETDEWEB)

    Kobayashi, Masaki, E-mail: masaki@tohtech.ac.jp; Kikuchi, Naoto; Sato, Akihiro [Department of Electronics and Intelligent Systems, Tohoku Institute of Technology, Sendai 982-8577 (Japan)

    2015-01-12

    This letter proposes and demonstrates ultrasound-combined optical imaging in dense scattering media. A peroxyoxalate chemiluminescence system that includes fluorophores to chemically excite the pigment is stimulated by ultrasound irradiation with power of less than 0.14 W/cm{sup 2}. Using focused ultrasound, the chemiluminescence is selectively spatially enhanced, which leads to imaging of the pigment when embedded in a light-scattering medium via scanning of the focal point. The ultrasonically enhanced intensity of the chemiluminescence depends on the base intensity of the chemiluminescence without the applied ultrasound irradiation, which thereby enables quantitative determination of the fluorophore concentration. The authors demonstrate the potential of this method to resolve chemiluminescent targets in a dense scattering medium that is comparable to biological tissue. An image was acquired of a chemiluminescent target that included indocyanine green as the fluorophore embedded at a depth of 20 mm in an Intralipid-10% 200 ml/l solution scattering medium (the reduced scattering coefficient was estimated to be approximately 1.3 mm{sup −1}), indicating the potential for expansion of this technique for use in biological applications.

  14. Electrochemistry and electrogenerated chemiluminescence from silicon nanocrystal quantum dots.

    Science.gov (United States)

    Ding, Zhifeng; Quinn, Bernadette M; Haram, Santosh K; Pell, Lindsay E; Korgel, Brian A; Bard, Allen J

    2002-05-17

    Reversible electrochemical injection of discrete numbers of electrons into sterically stabilized silicon nanocrystals (NCs) (approximately 2 to 4 nanometers in diameter) was observed by differential pulse voltammetry (DPV) in N,N'-dimethylformamide and acetonitrile. The electrochemical gap between the onset of electron injection and hole injection-related to the highest occupied and lowest unoccupied molecular orbitals-grew with decreasing nanocrystal size, and the DPV peak potentials above the onset for electron injection roughly correspond to expected Coulomb blockade or quantized double-layer charging energies. Electron transfer reactions between positively and negatively charged nanocrystals (or between charged nanocrystals and molecular redox-active coreactants) occurred that led to electron and hole annihilation, producing visible light. The electrogenerated chemiluminescence spectra exhibited a peak maximum at 640 nanometers, a significant red shift from the photoluminescence maximum (420 nanometers) of the same silicon NC solution. These results demonstrate that the chemical stability of silicon NCs could enable their use as redox-active macromolecular species with the combined optical and charging properties of semiconductor quantum dots.

  15. Chemiluminescence microarrays in analytical chemistry: a critical review.

    Science.gov (United States)

    Seidel, Michael; Niessner, Reinhard

    2014-09-01

    Multi-analyte immunoassays on microarrays and on multiplex DNA microarrays have been described for quantitative analysis of small organic molecules (e.g., antibiotics, drugs of abuse, small molecule toxins), proteins (e.g., antibodies or protein toxins), and microorganisms, viruses, and eukaryotic cells. In analytical chemistry, multi-analyte detection by use of analytical microarrays has become an innovative research topic because of the possibility of generating several sets of quantitative data for different analyte classes in a short time. Chemiluminescence (CL) microarrays are powerful tools for rapid multiplex analysis of complex matrices. A wide range of applications for CL microarrays is described in the literature dealing with analytical microarrays. The motivation for this review is to summarize the current state of CL-based analytical microarrays. Combining analysis of different compound classes on CL microarrays reduces analysis time, cost of reagents, and use of laboratory space. Applications are discussed, with examples from food safety, water safety, environmental monitoring, diagnostics, forensics, toxicology, and biosecurity. The potential and limitations of research on multiplex analysis by use of CL microarrays are discussed in this review.

  16. Evaluation of a new chemiluminescence immunoassay for diagnosis of syphilis

    Directory of Open Access Journals (Sweden)

    Mo Xiaohui

    2010-02-01

    Full Text Available Abstract Objective To assess the sensitivity, specificity, and feasibility of a new chemiluminescence immunoassay (CLIA in the diagnosis of syphilis. Methods At first, a retrospective study was conducted, using 135 documented cases of syphilis and 30 potentially interfering samples and 80 normal sera. A prospective study was also performed by testing 2, 071 unselected samples for routine screening for syphilis. CLIA was compared with a nontreponemal test (TRUST and a treponemal test (TPPA. Results There was an agreement of 100% between CLIA and TPPA in the respective study. The percentage of agreement among the 245 sera tested was 100.0%. Compared with TPPA, the specificity of CLIA was 99.9% (1817/1819, the sensitivity of CLIA was 100.0% (244/244 in the prospective study. CLIA showed 99.5% agreement with TPPA by testing 2, 071 unselected samples. And CLIA seemed to be more sensitive than TPPA in detecting the samples of primary syphilis. Conclusions CLIA is easy to perform and the indicator results are objective and unequivocal. It may be suitable for large-scale screening as a treponemal test substituted for TPPA.

  17. Capillary electrophoresis-chemiluminescence determination of norfloxacin and prulifloxacin

    Energy Technology Data Exchange (ETDEWEB)

    Yang Zhongju; Wang Xiaoli [College of Chemistry, Beijing Normal University, Beijing 100875 (China); Qin Weidong [College of Chemistry, Beijing Normal University, Beijing 100875 (China)], E-mail: qinwd@bnu.edu.cn; Zhao Huichun [College of Chemistry, Beijing Normal University, Beijing 100875 (China)], E-mail: zhaohuichun@bnu.edu.cn

    2008-08-15

    A capillary electrophoresis (CE)-chemiluminescence (CL) method for determining norfloxacin (NFLX) and prulifloxacin (PFLX) was developed based on the enhanced CL intensity of the cerium(IV)-sulfite-fluoroquinolone (FQ) reaction sensitized by terbium(III). The separation was conducted in buffer composed of 20 mM sodium citrate, 4 mM citric acid and 10 mM sodium sulfite at pH 6.1. The CL reagent solution consisted of 2 mM cerium(IV), 4 mM terbium(III) and 1.1 mM hydrochloric acid. NFLX and PFLX were baseline separated within 11 min with detection limits (S/N = 3) of 0.057 and 0.084 {mu}g mL{sup -1}, respectively. The maximum intra- and inter-day relative standard deviations (R.S.D.s) of migration time of the analytes were less than 4.0% and 4.2%, respectively. The proposed method was applied to detect NFLX and PFLX in fortified urine sample and the results were comparable to high-performance liquid chromatography (HPLC)-UV method. Moreover, the high selectivity of the CL detection and the high-separation efficiency of CE render the method the potential of quick analyzing fluoroquinolones in real complex matrix.

  18. Electrogenerated chemiluminescence of anatase TiO₂ nanotubes film.

    Science.gov (United States)

    Chen, Lifen; Lu, Lili; Mo, Yan; Xu, Zemin; Xie, Shunping; Yuan, Hongyan; Xiao, Dan; Choi, Martin M F

    2011-07-15

    Highly ordered titanium dioxide (TiO(2)) nanotubes film was successfully synthesized via anodic oxidation of a Ti foil in an ammonium fluoride-based ethylene glycol solution. The electrogenerated chemiluminescence (ECL) behavior of the resulting TiO(2) nanotubes film was subsequently studied. Strong ECL emission was observed at -1.40V (vs. Ag/AgCl) and the ECL spectrum displayed three emission peaks which were bathochromatically shifted by ca. 140nm as compared to its corresponding photoluminescence (PL) emission peaks, indicating that the surface state plays an important role in the emission process. The ECL emission can also occur in a deareated solution attributing to the surface adsorbed O(2) molecules. The ECL emission intensity was quenched by dopamine and greatly enhanced in the presence of dissolved O(2) and H(2)O(2), making it possible to detect these analytes. The TiO(2) nanotubes film has been successfully applied to determine the dissolved O(2) content in river and pond water samples, the H(2)O(2) concentration in commercial disinfectant samples and the dopamine concentration in commercial dopamine injections with satisfactory results. The plausible ECL mechanisms of TiO(2) nanotubes film in aqueous solution are discussed.

  19. Synthesis and properties of chemiluminescent acridinium ester labels with fluorous tags.

    Science.gov (United States)

    Natrajan, Anand; Wen, David; Sharpe, David

    2014-06-21

    Acridinium dimethylphenyl esters are highly sensitive chemiluminescent labels that are used in clinical diagnostics. Light emission from these labels is triggered with alkaline peroxide in the presence of the cationic surfactant cetyltrimethylammonium chloride (CTAC). CTAC compresses emission times of these labels to ester chemiluminescence (light yield) is quite sensitive to the polarity of the micellar interface. In the current study, we report the synthesis of new acridinium ester labels with fluorous tags of varying fluorine content and their chemiluminescence in the presence of cationic micelles of CTAC, anionic micelles of sodium perfluorooctanoate (SPFO) as well as mixed micelles of CTAC and SPFO. These studies indicate that in the presence of the mixed micelle system of CTAC and SPFO and at low mole fractions of SPFO, polarity of the mixed micelle interface is lower than that of CTAC leading to a greater enhancement of chemiluminescence for both fluorinated acridinium esters as well as a structurally analogous but non-fluorinated acridinium ester. Chemiluminescence stability of the fluorinated acridinium esters was either comparable to or better than the stability of the non-fluorinated acridinium ester. Non-specific binding to paramagnetic microparticles was higher for fluorinated acridinium esters requiring a surfactant wash to reduce their non-specific binding to the same extent as that observed for the non-fluorinated acridinium ester.

  20. Flow injection chemiluminescence determination of lercanidipine based on N-chlorosuccinimide-eosin Y post-chemiluminescence reaction.

    Science.gov (United States)

    Wang, Guowei; Zhao, Fang; Gao, Ying

    2014-12-01

    A novel post-chemiluminescence (PCL) reaction was discovered when lercanidipine was injected into the CL reaction mixture of N-chlorosuccinimide with alkaline eosin Y in the presence of cetyltrimethylammonium bromide (CTAB), where eosin Y was used as the CL reagent and CTAB as the surfactant. Based on this observation, a simple and highly sensitive PCL method combined with a flow injection (FI) technique was developed for the assay of lercanidipine. Under optimum conditions, the CL signal was linearly related to the concentration of lercanidipine in the range 7.0 × 10(-10) to 3.0 × 10(-6)  g/mL with a detection limit of 2.3 × 10(-10) g/mL (3σ). The relative standard deviation (RSD) was 2.1% for 1.0 × 10(-8) g/mL lercanidipine (n = 13). The proposed method had been applied to the estimation of lercanidipine in tablets and human serum samples with satisfactory results. The possible CL mechanism is also discussed briefly.

  1. [Changes in Kinetics of Chemiluminescence of Plasma as a Measure of Systemic Oxidative Stress in Humans].

    Science.gov (United States)

    Sozarukova, M M; Polimova, A M; Proskurnina, E V; Vladimirov, Yu A

    2016-01-01

    Oxidative stress is a pathogenetic factor of many diseases. The control of its level is important for early diagnosis and therapy adjustment. In this work, antioxidant status was estimated in blood plasma. In the system of 2,2'-azo-bis(2-amidinopropane)dihydrochloride-luminol a set of chemiluminescence kinetic curve parameters is proposed for oxidative stress level estimation (the latent period τ(lat) and the increasing of analytical signal ΔI(CL)). Uric acid and albumin were shown as the main components that responsible for changes in chemiluminescence kinetic curve of plasma. Serum albumin undergoes oxidative modification in dose-depend manner under the action of UV irradiation, it causes the enhancement of antioxidant properties. Changes in plasma chemiluminescence kinetics are proposed as a measure of oxidative stress in human body.

  2. Effect of fuel type on equivalence ratio measurements using chemiluminescence in premixed flames

    Science.gov (United States)

    Orain, Mikaël; Hardalupas, Yannis

    2010-05-01

    Local temporally-resolved measurements of chemiluminescent intensity from OH ∗, CH ∗ and C ∗2 radicals were obtained in premixed counterflow flames operating with propane and prevaporised fuels (isooctane, ethanol and methanol), for different equivalence ratios and strain rates. The results quantified independently the effects of fuel type, strain rate and equivalence ratio on chemiluminescent emissions from flames. The ability of chemiluminescent intensity from OH ∗, CH ∗ and C ∗2 radicals to indicate heat release rate depends strongly on fuel type. The intensity ratio OH ∗/CH ∗ has a monotonic decrease with equivalence ratio for all fuels and can be used to measure equivalence ratio of the reacting mixture. For propane and isooctane, the OH ∗/CH ∗ ratio remains independent of flame strain rate, whereas some dependence is observed for ethanol and methanol.

  3. Comparison of Chemiluminescence vs. Infrared Techniques for Detection of Fetuin-A in Saliva.

    Science.gov (United States)

    Mathews, Suresh T; Graff, Emily; Judd, Robert L; Kothari, Vishal

    2015-01-01

    The western blotting technique for transfer and detection of proteins, named following the discovery of southern and northern blotting for DNA- and RNA-blotting, respectively, has traditionally relied on the use of X-ray films to capture chemiluminescence. Recent advancements use super-cooled charge coupled devices (CCD) cameras to capture both chemiluminescence and fluorescence images, which exhibit a greater dynamic range compared to traditional X-ray film. Chemiluminescence detected by a CCD camera records photons and displays an image based on the amount of light generated as a result of a dynamic chemical reaction. Fluorescent detection with a CCD camera, on the other hand, is measured in a static state. Despite this advantage, researchers continue to widely use chemiluminescent detection methods due to the generally poor performance of fluorophores in the visible spectrum. Infrared imaging systems offer a solution to the dynamic reactions of chemiluminescence and the poor performance of fluorophores detected in the visible spectrum, by imaging fluorophores in the infrared spectrum. Infrared imaging is static, has a wide linear range, high sensitivity, and reduced autofluorescence and light scatter. A distinct advantage of infrared imaging is the ability to detect two target proteins simultaneously on the same blot which increases accuracy of quantification and comparison, while minimizing the need for stripping and reprobing. Here, we compare the methodology for chemiluminescent (UVP BioChemi) and infrared (UVP Odyssey) detection of salivary total and phosphorylated fetuin-A, a multifunctional protein associated with cardio-metabolic risk, and discuss the advantages and disadvantages of these methodologies.

  4. Assay of Cysteine in Human Serum with Quinine-Ce4+ Chemiluminescence System

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A sensitive and selective chemiluminescence (CL) method was developed for the determination of cysteine. This method is based on that the weak CL of cysteine oxidized with cerium (IV) can be greatly enhanced by quinine, and the total cysteine in human serum can be detected through simply diluting with water, showing a simpler analytical characteristic.

  5. Flow injection analysis of ketoprofen based on the order transform second chemiluminescence reaction

    Science.gov (United States)

    Zhuang, Yafeng; Cao, Guiping; Ge, Chuanqin

    2012-01-01

    This paper explores an order-transform-second-chemiluminescence (OTSCL) method combining the flow injection technique for the determination of ketoprofen. When ketoprofen solution was injected into the mixture after the end of the reaction of alkaline luminol and sodium periodate or sodium periodate solution was injected into the reaction mixture of ketoprofen and alkaline luminol, a new chemiluminescence (CL) reaction was initiated and strong CL signal was detected. A mechanism for the OTSCL has been proposed on the basis of the chemiluminescence kinetic characteristic, UV-visible absorption and chemiluminescent spectra. Under optimal experimental conditions, the CL response is proportional to the concentration of ketoprofen over the range of 2.0 × 10 -7 to 1.0 × 10 -5 mol/L with a correlation coefficient of 0.9950 and a detection limit of 8.0 × 10 -9 mol/L (3 σ). The relative standard deviation for 11 repetitive determinations of 1.0 × 10 -6 mol/L ketoprofen is 2.9%. The utility of this method was demonstrated by determining ketoprofen in pharmaceutical formulations without interference from its potential impurities.

  6. Design and evaluation of capillary electrophoresis in dynamically coated capillaries coupled with chemiluminescence detection.

    Science.gov (United States)

    Liu, Haiyan; Han, Ning; Zhang, Lingyi; Du, Yiping; Zhang, Weibing

    2010-11-08

    A dynamic coating capillary electrophoresis coupled with a simplified on-line chemiluminescence detection system was designed and evaluated. In the proposed system, poly-vinylpyrrolidone was used as dynamic coating substance in the separation buffer to reduce the unwanted protein non-specific adsorption, which was first applied in capillary electrophoresis coupling with on-line chemiluminescence detection. In order to avoid complex processing, an ordinary plastic cuvette was modified as a three-way joint. The chemiluminescence reaction conditions and capillary electrophoresis separation conditions were investigated in detail. The results showed that the coated capillary can be injected protein samples at least 30 times continuously with good repeatability. Under optimal conditions, the chemiluminescence relative intensity was linear with the concentration of hemoglobin in the range of 4-1850 μg mL(-1) and the detection limit was 2.0 μg mL(-1) (S/N=3). The relative standard deviation of migration times and peak heights for 40 μg mL(-1) hemoglobin were 2.5% and 4.1% (n=11) respectively. Interference of matrix effects was overcome by the calibration according to standard addition methods. Afterwards, the method was validated successfully and was applied to detect the concentration of hemoglobin in the serum of haemolytic patients.

  7. Measurement of thyrotropin in clinical and subclinical hyperthyroidism using a new chemiluminescent assay.

    Science.gov (United States)

    Ross, D S; Ardisson, L J; Meskell, M J

    1989-09-01

    A new commercially available chemiluminescent TSH assay demonstrates an 8- to 10-fold increase in sensitivity over a sensitive immunoradiometric assay, allowing increased ability to distinguish partial from more complete thyrotroph suppression. Sera were analyzed from 145 patients who had TSH concentrations below 0.08 mU/L in the immunoradiometric assay. Most patients with overt hyperthyroidism had undetectable TSH concentrations in the chemiluminescent assay. Three groups of patients were identified, in which a large subgroup had undetectable values in the immunoradiometric assay and detectable values in the chemiluminescent assay (12 of 17 patients under treatment for hyperthyroidism who had recently normalized their serum thyroid hormone levels, 33 of 68 patients taking L-T4, and 4 of 8 patients with endogenous subclinical hyperthyroidism). In addition, several patients with undetectable basal and detectable TRH-stimulated TSH values in the immunoradiometric assay had detectable basal TSH values in the chemiluminescent assay. The utility of first generation TSH immunometric assays was their ability to distinguish hyperthyroidism from euthyroidism. The clinical utility of increasingly sensitive TSH assays will be to distinguish degrees of thyrotroph suppression in subclinical hyperthyroidism.

  8. A novel chemiluminescent immunoassay for detection of Toxoplasma gondii IgG in human sera.

    Science.gov (United States)

    Holec-Gąsior, Lucyna; Ferra, Bartłomiej; Czechowska, Justyna; Serdiuk, Illia E; Krzymiński, Karol; Kur, Józef

    2016-08-01

    This study describes Toxoplasma gondii IgG chemiluminescent immunoassay (CLIA) based on the use of a novel immunochemical reagents in the form of the conjugates of original acridinium ester (AE) labels attached to antibodies and SAG2-GRA1-ROP1L chimeric antigen and shows that this test is useful for diagnostic purposes.

  9. Evaluation of endotoxin (LPS) activity in bovine blood using neutrophil dependent chemiluminescence

    Science.gov (United States)

    The purpose of this study was to evaluate the applicability of a neutrophil chemiluminescence-based assay for the measurement of LPS stimulatory activity in bovine whole blood. The assay is based on the capacity for LPS to trigger the respiratory oxidative burst activity (RBA) of autologous neutroph...

  10. Chemiluminescence: Measuring methods. (Latest citations from the NTIS bibliographic database). Published Search

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-05-01

    The bibliography contains citations concerning chemiluminescence assays. The citations include sample system design, sample collection, measurement techniques, and sensitivity of the instrumentation. Applications in high altitude air pollution studies are emphasized. (Contains 50-250 citations and includes a subject term index and title list.) (Copyright NERAC, Inc. 1995)

  11. Fabrication technology of chemiluminescent sensitive elements for rocket-borne ozone detectors

    Science.gov (United States)

    Kononkov, V. A.; Lelikova, A. I.; Perov, S. P.

    Attention is given to the technology behind the fabrication of chemiluminescent sensitive elements for rocket-borne ozone detectors. High-silica microporous glass is the basic material required for these detectors. It is noted that the luminophor consists of rhodamine-C and gallic acid, and that the desired ratio of these components depends on the sensitivity of a particular specimen to ozone.

  12. Acidic Potassium Permanganate Chemiluminescence for the Determination of Antioxidant Potential in Three Cultivars of Ocimum basilicum.

    Science.gov (United States)

    Srivastava, Shivani; Adholeya, Alok; Conlan, Xavier A; Cahill, David M

    2016-03-01

    Ocimum basilicum, a member of the family Lamiaceae, is a rich source of polyphenolics that have antioxidant properties. The present study describes the development and application of an online HPLC-coupled acidic potassium permanganate chemiluminescence assay for the qualitative and quantitative assessment of antioxidants in three cultivars of O. basilicum grown under greenhouse conditions. The chemiluminescence based assay was found to be a sensitive and efficient method for assessment of total and individual compound antioxidant potential. Leaves, flowers and roots were found to be rich reserves of the antioxidant compounds which showed intense chemiluminescence signals. The polyphenolics such as rosmarinic, chicoric, caffeic, p-coumaric, m-coumaric and ferulic acids showed antioxidant activity. Further, rosmarinic acid was found to be the major antioxidant component in water-ethanol extracts. The highest levels of rosmarinic acid was found in the leaves and roots of cultivars "holy green" (14.37; 11.52 mM/100 g DW respectively) followed by "red rubin" (10.02; 10.75 mM/100 g DW respectively) and "subja" (6.59; 4.97 mM/100 g DW respectively). The sensitivity, efficiency and ease of use of the chemiluminescence based assay should now be considered for its use as a primary method for the identification and quantification of antioxidants in plant extracts.

  13. Chemiluminescence in the reaction of ozone with 6-methyluracil in aqueous solutions

    Science.gov (United States)

    Zimin, Yu. S.; Khairullina, L. R.; Kutlugil'dina, G. G.; Mustafin, A. G.

    2015-12-01

    Chemiluminescence in the visible part of the spectrum during the oxidation of 6-methyluracil with ozone in aqueous solutions was found. The kinetics of variation of the luminescence intensity was studied in the temperature range 287-333 K, and the activation parameters of the process were determined.

  14. A new label-free strategy for a highly efficient chemiluminescence immunoassay.

    Science.gov (United States)

    Yang, Zhanjun; Cao, Yue; Li, Juan; Wang, Juntao; Du, Dan; Hu, Xiaoya; Lin, Yuehe

    2015-10-01

    A new label-free chemiluminescence (CL) immunoassay method is proposed for the cheap, convenient and sensitive detection of proteins through the co-immobilization of a capture antibody and horseradish peroxidase on a sensing interface. The specific immunoreaction on the interface effectively inhibits the enzymatic CL reaction, thus causing a decrease in CL signals.

  15. Soot and chemiluminescence in diesel combustion of bio-derived, oxygenated and reference fuels

    NARCIS (Netherlands)

    Klein-Douwel, R.J.H.; Donkerbroek, A.J.; Vliet, A.P. van; Boot, M.D.; Somers, L.M.T.; Baert, R.S.G.; Dam, N.J.; Meulen, J.J. ter

    2009-01-01

    High-speed imaging, spectroscopy and thermodynamical characterization are applied to an optically accessible, heavy-duty diesel engine in order to compare sooting and chemiluminescence behaviour of bio-derived, oxygenated fuels and various reference fuels. The fuels concerned include the bio-derived

  16. Luminol/antibody labeled gold nanoparticles for chemiluminescence immunoassay of carcinoembryonic antigen

    Energy Technology Data Exchange (ETDEWEB)

    Yang Xiaoyan, E-mail: yangxiaoyan_zh@126.com [Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042 (China); Guo Yingshu; Wang Aiguo [Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042 (China)

    2010-05-07

    A facile strategy by loading luminol and secondary antibody on gold nanoparticles (Au NPs) was described in the present work. The as-prepared luminol/antibody labeled Au NPs conjugates (LAAu NPs) were used as the chemiluminescent probe for the detection of carcinoembryonic antigen (CEA) in serum. The LAAu NPs were characterized by transmission electron microscopy (TEM), UV-vis spectrophotometry (UV-vis), and chemiluminescent method. Stable and efficient chemiluminescence (CL) was obtained when luminol molecules and secondary antibodies were coimmobilized on the Au NPs by using hydrogen peroxide (H{sub 2}O{sub 2}) as an oxidant, horseradish peroxidase (HRP) as a catalyst, and 4-(4'-iodo)phenylphenol (IPP) as an enhancer. The LAAu NPs were further evaluated via a sandwich-type CL immunoassay of CEA in serum. In this protocol, the CEA analyte was captured by the primary antibody immobilized on the surface of magnetic beads, and then was sandwiched by the secondary antibody loaded on luminol-labeled Au NPs. The chemiluminescent intensity was proportional to the concentration of CEA over the range of 5.0 x 10{sup -10} to 5.0 x 10{sup -8} g mL{sup -1} and 5.0 x 10{sup -9} to 2.0 x 10{sup -8} g mL{sup -1} by using HRP and Co{sup 2+} as catalysts, respectively. The present chemiluminescent immunoassay based on the luminol/antibody labeled Au NPs conjugates has offered great promise for simple, highly biocompatible, and cost-effective analysis of biological samples.

  17. Bio-bar-code functionalized magnetic nanoparticle label for ultrasensitive flow injection chemiluminescence detection of DNA hybridization.

    Science.gov (United States)

    Bi, Sai; Zhou, Hong; Zhang, Shusheng

    2009-10-07

    A signal amplification strategy based on bio-bar-code functionalized magnetic nanoparticles as labels holds promise to improve the sensitivity and detection limit of the detection of DNA hybridization and single-nucleotide polymorphisms by flow injection chemiluminescence assays.

  18. Development and characterization of a microfluidic glucose sensing system based on an enzymatic microreactor and chemiluminescence detection

    NARCIS (Netherlands)

    Moon, B. -U; de Vries, M. G.; Westerink, B. H. C.; Verpoorte, E.

    2012-01-01

    Chemiluminescence detection was developed as an alternative to amperometric detection for glucose analysis in a portable, microfluidics-based continuous glucose monitoring system. Amperometric detection allows easy determination of hydrogen peroxide, a product of the glucose oxidase-catalyzed reacti

  19. Simultaneous Determination of Gold and Platinum by Double Artificial Neural Network Analysis with Flow-injection Chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    Ming Yang LIU; Hai Tao ZHANG; Jun Feng LI; Shu Gui CHEN; Hong Yan WANG

    2006-01-01

    A highly sensitive double artificial neural network (DANN) analysis with flow-injection chemiluminescence (FI-CL) has been developed to simultaneously determine the trace amounts of the gold and platinum in simulated mixed samples, without the boring process.

  20. Chemiluminescence assay for catechin based on generation of hydrogen peroxide in basic solution

    Energy Technology Data Exchange (ETDEWEB)

    Arakawa, Hidetoshi; Kanemitsu, Mahina; Tajima, Noriko; Maeda, Masako

    2002-11-20

    We have determined that the catechin group in basic solution efficiently produces hydrogen peroxide; moreover, a highly sensitive analysis methodology was developed to measure catechin employing a peroxalate chemiluminescence detection system. Identification of hydrogen peroxide generated by catechin was determined by ESR as well as peroxalate chemiluminescence using catalase and SOD. As a result, catechin-generated superoxide by electron reduction to dissolved oxygen in basic solution, followed by production of hydrogen peroxide through dismutation reaction. This method could measure several tea catechins, (+)-catechin (CC), (-)-epigallocatechin-3-gallate (EGCg), (-)-epicatechin-3-gallate (ECG) and gallic acid, with measurement range from 10{sup -7} to 10{sup -3} mol/l and sensitivity of 10{sup -8} mol/l. This method was also applied to the determination of total catechin levels in green tea, black tea and roasted green tea.

  1. Chemiluminescent Nanomicelles for Imaging Hydrogen Peroxide and Self-Therapy in Photodynamic Therapy

    Directory of Open Access Journals (Sweden)

    Rui Chen

    2011-01-01

    Full Text Available Hydrogen peroxide is a signal molecule of the tumor, and its overproduction makes a higher concentration in tumor tissue compared to normal tissue. Based on the fact that peroxalates can make chemiluminescence with a high efficiency in the presence of hydrogen peroxide, we developed nanomicelles composed of peroxalate ester oligomers and fluorescent dyes, called peroxalate nanomicelles (POMs, which could image hydrogen peroxide with high sensitivity and stability. The potential application of the POMs in photodynamic therapy (PDT for cancer was also investigated. It was found that the PDT-drug-loaded POMs were sensitive to hydrogen peroxide, and the PDT drug could be stimulated by the chemiluminescence from the reaction between POMs and hydrogen peroxide, which carried on a self-therapy of the tumor without the additional laser light resource.

  2. Recent analytical applications of nanoparticle sensitized lucigenin and luminol chemiluminescent reactions

    Indian Academy of Sciences (India)

    S M Wabaidur; Mu Naushad; Z A Alothman

    2012-02-01

    There is an ever-increasing demand for rapid, sensitive, cost effective and selective detection methods for the analysis of many essential compounds.When chemiluminescence has been introduced to analytical chemistry as a detection technique, it has been shown to meet many of these requirements. This method has become a powerful tool for the determination of many compounds. Using this method, low detection limits can be obtained with simple and inexpensive instrumentation. Coupled with flow injection technique the method has become more popular for wider applications. Since many excellent reviews on the chemiluminogenic techniques have appeared in the literature in recent years, the present paper does not intend to cover the exhaustive studies in this area, but will selectively describe the analytical applications of nanoparticle sensitized lucigenin and luminol chemiluminescent reactions and evaluate their recent progress together with our present work.

  3. Bioinspired photonic structures by the reflector layer of firefly lantern for highly efficient chemiluminescence

    Science.gov (United States)

    Chen, Linfeng; Shi, Xiaodi; Li, Mingzhu; Hu, Junping; Sun, Shufeng; Su, Bin; Wen, Yongqiang; Han, Dong; Jiang, Lei; Song, Yanlin

    2015-08-01

    Fireflies have drawn considerable attention for thousands of years due to their highly efficient bioluminescence, which is important for fundamental research and photonic applications. However, there are few reports on the reflector layer (RL) of firefly lantern, which contributes to the bright luminescence. Here we presented the detailed microstructure of the RL consisting of random hollow granules, which had high reflectance in the range from 450 nm to 800 nm. Inspired by the firefly lantern, artificial films with high reflectance in the visible region were fabricated using hollow silica microparticles mimicking the structure of the RL. Additionally, the bioinspired structures provided an efficient RL for the chemiluminescence system and could substantially enhance the initial chemiluminescence intensity. The work not only provides new insight into the bright bioluminescence of fireflies, but also is importance for the design of photonic materials for theranostics, detection, and imaging.

  4. Analysis of Phenolic Compounds in Coke Plant Wastewater by Capillary Zone Electrophoresis with Inhibited Chemiluminescence Detection

    Institute of Scientific and Technical Information of China (English)

    Xiang Dong XU; Yong Gang HU; Ze Yu YANG

    2006-01-01

    A capillary electrophoresis(CE) with on-line inhibited chemiluminescence (CL) detection was firstly used for the simultaneous analysis of benzenediol isomers and phenol. It is based on the quenching effect of benzenediol isomers and phenol on the chemiluminescence reaction of luminol with potassium ferricyanide in sodium hydroxide medium. Under the optimum conditions, the four phenols were baseline separated and detected in less than 10 min.The detection limits (S/N=3) for hydroquinone, resorcinol, catechol and phenol were 2.9×10-8mol/L, 3.7×10-7 mol/L, 8.4×10-8 mol/L and 4.4×10-6 mol/L, respectively. Finally, the presented method has been successfully applied to real sample.

  5. Evaluation of serum antioxidant activity in patients with liver pathology by the chemiluminescent method.

    Science.gov (United States)

    Titov, V N; Sazhina, N N

    2014-12-01

    Total antioxidant activity of the serum in patients with liver pathology was assessed by two chemiluminescent methods based on different models of free radical oxidation: Hb-H2O2-luminol and ABAP-luminol. Comparative analysis showed a significant, but not high correlation of the results (r=0.798), which can be explained by different mechanisms of induction of free radicals and effects of various serum components (proteins and bilirubin) on the initiation process. The influence of aphysiological concentration of analyzed values manifested in the Hb-H2O2 model. Disagreement between the results of measurements was more pronounced in patients with abnormally high serum bilirubin content. The results suggested that ABAP-luminol chemiluminescent model is more preferable for evaluation of antioxidant activity in clinical practice.

  6. Kinetics simulation of luminol chemiluminescence based on quantitative analysis of photons generated in electrochemical oxidation.

    Science.gov (United States)

    Koizumi, Yozo; Nosaka, Yoshio

    2013-08-22

    The kinetics of electrogenerated chemiluminescence (ECL) of luminol at a gold electrode in alkaline solution was investigated by measuring the absolute number of photons emitted in an integrating sphere. The ECL efficiency as the ratio of photon to electric charge was 0.0004 in cyclic voltammography and 0.0005 in chronoamperometry. By numerically solving the rate equations based on a diffusion layer model, the observed time profile of the luminescence intensity could be successfully simulated from the oxidation current of luminol in the chronoamperometry. In the simulation, the rate constant for the oxidation of luminol by superoxide radicals in alkaline solution was determined to be 6 × 10(5) M(-1) s(-1). The present methodology and the achievement could be widely applicable to various analytical techniques using chemiluminescence.

  7. Determination of resveratrol in red wine by solid phase extraction-flow injection chemiluminescence method

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A sensitive flow injection chemiluminescence method has been developed for the detection of resveratrol in red wine based on the fact that resveratrol can greatly enhance chemiluminescence reaction between KMnO4 and HCHO in sulfuric acid medium.Analytes were pre-concentrated on solid sorbents (C18 solid-phase extraction cartridges). Under the optimum conditions, the proposed method allows the measurement of resveratrol over the range of 1.32 × 10-s to 1.32 × 10-5 mol/L with a detection limit of 3.30 × 10-9 mol/L, and the relative standard deviation for 1.32 × 10-5 mol/L resveratrol (n = 11 ) is 3.8%. This method has been successfully applied for the determination of the resveratrol in red wine. Furthermore, the possible reaction mechanism was also discussed.

  8. Chemiluminescence Determination of Benzoic Acid Using A Solid-Phase Verdigris Reactor

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    A new chemiluminescence flow system has been developed for sequential determina-tion of benzoic acid based on the reaction of the compound with copper carbonate entrapped in a solid-phase reactor. It was found that the unsaturated complex of Cu(II) and benzoic acid (1:1) has strong catalytic effect on the luminol-H2O2 chemiluminescence reaction. The calibration graph is linear over the range of 0.025 ~ 60 μg/mL of benzoic acid, with a relative standard deviation of less than 3.0 %, and the detection limit is 0.01μg@mL-1. The proposed method was applied to the determination of benzoic acid content in different pharmaceutical formulations.

  9. Determination of glyphosate in foodstuff by one novel chemiluminescence-molecular imprinting sensor

    Science.gov (United States)

    Zhao, Peini; Yan, Mei; Zhang, Congcong; Peng, Ruixue; Ma, Dongsheng; Yu, Jinghua

    2011-05-01

    A novel chemiluminescence (CL) sensor for the determination of glyphosate (GLY) was made up based on molecularly imprinted polymer (MIP). The molecularly imprinted microspheres (MIMs) with a small dimension which possess extremely high surface-to-volume ratio were synthesized using precipitation polymerization with GLY as template. And then the MIMs were modified on glass sheets, which were placed at the bottom of wells of microplate as the recognizer. Subsequently, a highly selective and high throughput chemiluminescence (CL)-molecular imprinting (MI) sensor for detection of GLY was achieved. Influencing factors were investigated and optimized in detail. The method can perform 96 independent measurements sequentially in 10 min and the limit of detection (LOD) for GLY was 0.046 μg mL -1. The relative standard deviation (RSD) for 11 parallel measurements of GLY was 4.68%. The results show that CL-MI sensor can become a useful analytical technology for quick molecular recognition.

  10. Determination of captopril by high-performance liquid chromatography with direct electrogenerated chemiluminescence

    Science.gov (United States)

    Sun, Yonghua; Zhang, Zhujun; Zhang, Xinfeng

    2013-03-01

    Captopril exhibit electrogenerated chemiluminescence (ECL) in NaNO3 solution when constant current is exerted. Based on this observation, a direct ECL method coupled with high-performance liquid chromatography (HPLC) separation is developed for determination of captopril in human serum. Factors affected the ECL emission are investigated. Under the optimal conditions, the ECL intensity has a linear relationship with the concentration of captopril in the range of 4.0 × 10-6-2.0 × 10-3 g mL-1 and the detection limit is 2 × 10-6 g mL-1 (S/N = 3). Compared with the common electrogenerated chemiluminescence experiments, the developed method need no any other fluorescence additives.

  11. Advances in the use of acidic potassium permanganate as a chemiluminescence reagent: A review

    Energy Technology Data Exchange (ETDEWEB)

    Adcock, Jacqui L.; Barnett, Neil W.; Barrow, Colin J.; Francis, Paul S., E-mail: psf@deakin.edu.au

    2014-01-07

    Graphical abstract: -- Highlights: •Analytical applications of acidic potassium permanganate chemiluminescence. •Discussion of emitting species and light-producing reaction pathways. •Influence of enhancers such as polyphosphates, formaldehyde and sulfite. •Clinical, forensic, food science, agricultural and environmental applications. -- Abstract: We review the analytical applications of acidic potassium permanganate chemiluminescence published since our previous comprehensive review in mid-2007 to early 2013. This includes a critical evaluation of evidence for the emitting species, the influence of additives such as polyphosphates, formaldehyde, sulfite, thiosulfate, lanthanide complexes and nanoparticles, the development of a generalized reaction mechanism, and the use of this chemistry in pharmaceutical, clinical, forensic, food science, agricultural and environmental applications.

  12. Flow-injection chemiluminescence determination of tryptophan using galangin-potassium permanganate-polyphosphoric acid system

    Institute of Scientific and Technical Information of China (English)

    Hui Chen; Li Li; Min Zhou; Yong Jun Ma

    2008-01-01

    A high sensitive flow-injection chemiluminescence (FI-CL)method for the determination of tryptephan has been developed.The method is based on the chemiluminescence reaction of galangin-potassium perrnanganate-tryptophan in polyphosphoric acid (PPA)media.Under the optimized conditions,tryptophan was determined in the range 0.05-10 μg/mL with the detection limit (30)of 5.0× 10-3 μg/mL.The relative standard deviation (RSD)was 1.0% for 11 replicate determinations of 1.0 μg/mL tryptophan.Three synthetic samples were determined selectively with recoveries in the range from 99.6% to 102.0% in the presence of other amino acids.

  13. Detecting free radicals in sunscreens exposed to UVA radiation using chemiluminescence.

    Science.gov (United States)

    Millington, Keith R; Osmond, Megan J; McCall, Maxine J

    2014-04-05

    One of the current concerns with the application of nanoparticles in sunscreens, and in particular nano-TiO2 and ZnO, is their potential to photogenerate free radicals and reactive oxygen species (ROS) when they absorb ultraviolet wavelengths from sunlight. Free radicals and ROS are known to be associated with UV-induced skin damage and oxidative stress, from which sunscreens are expected to offer significant protection. Here we describe a simple method, based on chemiluminescence emission, for detecting free radicals generated in commercial sunscreens alone, and when applied to various substrates, following exposure to UVA (320-400nm) radiation. This photo-induced chemiluminescence (PICL) technique could be used to optimise sunscreen formulations so as to minimise free radical photogeneration during exposure to sunlight.

  14. H2S Analysis in Biological Samples Using Gas Chromatography with Sulfur Chemiluminescence Detection

    OpenAIRE

    Vitvitsky, Victor; Banerjee, Ruma

    2015-01-01

    Hydrogen sulfide (H2S) is a metabolite and signaling molecule in biological tissues that regulates many physiological processes. Reliable and sensitive methods for H2S analysis are necessary for a better understanding of H2S biology and for the pharmacological modulation of H2S levels in vivo. In this chapter, we describe the use of gas chromatography coupled to sulfur chemiluminescence detection to measure the rates of H2S production and degradation by tissue homogenates at physiologically r...

  15. A Fully Automated Sequential-Injection Analyser for Dual Electrogenerated Chemiluminescence/Amperometric Detection

    OpenAIRE

    Economou, Anastasios; Nika, Maria

    2006-01-01

    This work describes the development of a dedicated, fully automated sequential-injection analysis (SIA) apparatus suitable for simultaneous electrogenerated chemiluminescence (ECL) and amperometric detection. The instrument is composed of a peristaltic pump, a multiposition selection valve, a home-made potentiostat, a thin-layer electrochemical/optical flow-through cell, and a light detector. Control of the experimental sequence and simultaneous data acquisition of the light and the current i...

  16. New method for the photo-chemiluminometric determination of benzoylurea insecticides based on acetonitrile chemiluminescence.

    Science.gov (United States)

    Gil García, M D; Martínez Galera, M; Santiago Valverde, R

    2007-03-01

    The viability of tandem photochemical reaction-chemiluminescence detection has been studied for the determination of five benzoylurea insecticides, namely, diflubenzuron, triflumuron, hexaflumuron, lufenuron and flufenoxuron. The 'on-line' photochemical reaction of benzoylurea pesticides provides an enhanced chemiluminescence response of the pesticides during their oxidation by potassium hexacyanoferrate(III) and sodium hydroxide, whose signal increases with the percentage of acetonitrile in the reaction medium. The determination was performed using a photoreactor consisting of a PFA (perfluoroalkoxy) tube reactor coil (5 mx1.6-mm O.D. and 0.8-mm I.D.) and an 8-W xenon lamp. As the yield of the photoderivatization process and the chemiluminescent signals depend on the percentage of acetonitrile, the chromatographic column (a Gemini C18, Phenomenex 150 mmx4.6 mm, 5-microm particle size) was chosen with the aim of using high percentages of this organic solvent in the mobile phase. Previous studies showed that the rate of the chemiluminescent reaction was very fast. Therefore, a modification was carried out in the detector in order to mix the analytes and reactants as near as possible to the measure cell. The optimised method was validated with respect to linearity, precision, limits of detection and quantification accuracy. Under the optimised conditions, linear working range extends three orders of magnitude with the relative standard deviation of intra-day precision below 10% and detection limits between 0.012 and 0.18 microg mL-1, according to the compound. The proposed method has been successfully applied to the determination of benzoylureas in cucumber with good results.

  17. Microfluidic cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection of viral DNA

    Directory of Open Access Journals (Sweden)

    M. Zangheri

    2016-03-01

    With this system, taking advantage of oligoprobes specificity, chemiluminescence detectability, and photosensor sensitivity, accurate quantification of target analytes down to 70 pmol L−1 was obtained for each B19 DNA genotype, with high specificity and multiplexing ability. Results confirm the good detection capabilities and assay applicability of the proposed system, prompting the development of innovative portable analytical devices with enhanced sensitivity and multiplexed capabilities.

  18. Mechanisms of bioluminescence, chemiluminescence and of their regulation. Progress report, one year period through March 1976

    Energy Technology Data Exchange (ETDEWEB)

    Seliger, H H

    1976-01-01

    Progress is reported on a 10-yr study of the production and role of excited states in biological systems and the mechanisms involved in bioluminescence and chemoluminescence. An hypothesis of the origin of bioluminescence is presented that is based on the mixed function oxygenase reaction. Techniques of absolute measurements of light intensities and spectral composition were applied in studies of bioluminescence of marine dinoflagellates and the chemiluminescence of carcinogenic polycyclic aromatic hydrocarbons as the result of enzymatic hydroxylation. (CH)

  19. Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2O2)-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-250 ng mL·1. The detection limit of CEA was 0.61 ng mL·1. The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.

  20. Insights into the antioxidant activity of some flavones on silver nanoparticles using the chemiluminescence method

    Energy Technology Data Exchange (ETDEWEB)

    Voicescu, Mariana, E-mail: voicescu@icf.ro [Romanian Academy, Institute of Physical Chemistry “Ilie Murgulescu”, Splaiul Independentei 202, 060021 Bucharest (Romania); Nistor, Cristina L. [Polymer Department, National R and D Institute for Chemistry and Petrochemistry ICECHIM, Splaiul Independentei 202, 060021 Bucharest (Romania); Meghea, Aurelia [University POLITEHNICA of Bucharest, Faculty of Applied Chemistry and Materials Sciences, Polizu 1, 78126 Bucharest (Romania)

    2015-01-15

    The work aims to simulate in vitro the effects caused by oxidation of five hydroxyflavones (HF) (some typical models of flavonols), (3-HF, 6-HF, 7-HF, 3,6-diHF and 3,7-diHF) on silver nanoparticles (SNPs) using the chemiluminescent system luminol–hydrogen peroxide, in phosphate buffer, pH 7.4. The contribution of bovine and human serum albumins to the antioxidant activity of the mentioned flavones, and the effect on the SNPs support, in the chemiluminescent system luminol–hydrogen peroxide, has been also investigated. The results are discussed with relevance to the oxidative stress process. - Highlights: • The effects caused by oxidation of five hydroxiflavones (HF) (3-HF, 6-HF, 7-HF, 3,6-diHF and 3,7-diHF) on silver nanoparticles (SNPs) using the chemiluminescent (CL) system luminol–hydrogen peroxide, in phosphate buffer, pH 7.4. • The contribution of bovine and human serum albumins to the antioxidant activity of the mentioned flavones, and the effect on the SNPs support, in the CL system luminol–hydrogen peroxide, are discussed. • The results have relevance to the oxidative stress process.

  1. A streptavidin functionalized graphene oxide/Au nanoparticles composite for the construction of sensitive chemiluminescent immunosensor

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Zhanjun, E-mail: zjyang@yzu.edu.cn [Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002 (China); Luo, Shufen; Li, Juan; Shen, Juan; Yu, Suhua; Hu, Xiaoya [Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002 (China); Dionysiou, Dionysios D. [Environmental Engineering and Science Program, School of Energy Environmental, Biological and Medical Engineering, University of Cincinnati, Cincinnati, OH 45221-0012 (United States)

    2014-08-11

    Highlights: • A novel streptavidin/GO/AuNPs composite is prepared for immobilizing antibody. • A highly sensitive chemiluminescent immunosensor is constructed for tumor marker. • The immunoassay system shows extremely low detection limit down to picogram level. • This work provides a promising approach for ultrasensitive biosensing applications. - Abstract: In this work, a novel streptavidin functionalized graphene oxide/Au nanoparticles (streptavidin/GO/AuNPs) composite is prepared and for the first time used to construct sensitive chemiluminescent immunosensor for the detection of tumor marker. The streptavidin/GO/AuNPs composite and the immunosensor are characterized using scanning electron microscopy, static water contact angle measurement and electrochemical impedance spectroscopy. The biofunctionalized composite has large reactive surface area and excellent biocompatibility, thus the capture antibody can be efficiently immobilized on its surface based on the highly selective recognition of streptavidin to biotinylated antibody. Using α-fetoprotein (AFP) as a model, the proposed chemiluminescent immunosensor shows a wide linear range from 0.001 to 0.1 ng mL{sup −1} with an extremely low detection limit down to 0.61 pg mL{sup −1}. The resulting AFP immunosensor shows high detection sensitivity, fast assay speed, acceptable detection and fabrication reproducibility, good specificity and stability. The assay results of serum samples with the proposed method are in an acceptable agreement with the reference values. This work provides a promising biofunctionalized nanostructure for sensitive biosensing applications.

  2. Simultaneous determination of chloramphenicol, florfenicol and florfenicol amine in ham sausage with a hybrid chemiluminescent immunoassay.

    Science.gov (United States)

    Tao, Xiaoqi; Jiang, Haiyang; Yu, Xuezhi; Zhu, Jinghui; Wang, Xia; Wang, Zhanhui; Niu, Lanlan; Wu, Xiaoping; Shen, Jianzhong

    2013-01-01

    A novel chemiluminescent immunoassay utilising two types of primary antibodies (murine monoclonal antibody and rabbit polyclonal antibody) and two types of horseradish peroxidase-labelled secondary antibodies was established for simultaneously detecting multiple amphenicol residues in ham sausage. After combining the extract procedure of the target amphenicol into one simplified method, this hybrid chemiluminescent immunoassay could screen chloramphenicol (CAP), florfenicol (FF) and its metabolite florfenicol amine (FFA) at the same time by adding the corresponding secondary antibody. Ham sausage samples were analysed by using this hybrid immunoassay, with LODs of CAP being 0.01 μg kg⁻¹, of FF being 2.8 μg kg⁻¹ and of FFA being 3.0 μg kg⁻¹. The applicability of the proposed method has been validated by determining CAP, FF and FFA in ham sausage samples with satisfactory results. Good recoveries and high correlation with traditional enzyme-linked immunosorbent assay and LC-MS/MS results illustrated that the developed hybrid chemiluminescent immunoassay could screen high-throughput ultra-trace amphenicol residues effectively at one time.

  3. Reagentless chemiluminescence-based fiber optic sensors for regenerative life support in space

    Science.gov (United States)

    Atwater, James E.; Akse, James R.; DeHart, Jeffrey; Wheeler, Richard R., Jr.

    1995-04-01

    The initial feasibility demonstration of a reagentless chemiluminescence based fiber optic sensor technology for use in advanced regenerative life support applications in space and planetary outposts is described. The primary constraints for extraterrestrial deployment of any technology are compatibility with microgravity and hypogravity environments; minimal size, weight, and power consumption; and minimal use of expendables due to the great expense and difficulty inherent to resupply logistics. In the current research, we report the integration of solid state flow through modules for the production of aqueous phase reagents into an integrated system for the detection of important analytes by chemiluminescence, with fiber optic light transmission. By minimizing the need for resupply expendables, the use of solid phase modules makes complex chemical detection schemes practical. For the proof of concept, hydrogen peroxide and glucose were chosen as analytes. The reaction is catalyzed by glucose oxidase, an immobilized enzyme. The aqueous phase chemistry required for sensor operation is implemented using solid phase modules which adjust the pH of the influent stream, catalyze the oxidation of analyte, and provide the controlled addition of the luminophore to the flowing aqueous stream. Precise control of the pH has proven essential for the long-term sustained release of the luminophore. Electrocatalysis is achieved using a controlled potential across gold mesh and gold foil electrodes which undergo periodic polarity reversals. The development and initial characterization of performance of the reagentless fiber optic chemiluminescence sensors are presented in this paper.

  4. Selective and Sensitive Chemiluminescence Determination of MCPB: Flow Injection and Liquid Chromatography.

    Science.gov (United States)

    Meseguer-Lloret, Susana; Torres-Cartas, Sagrario; Catalá-Icardo, Mónica; Gómez-Benito, Carmen

    2016-02-01

    Two new chemiluminescence (CL) methods are described for the determination of the herbicide 4-(4-chloro-o-tolyloxy) butyric acid (MCPB). First, a flow injection chemiluminescence (FI-CL) method is proposed. In this method, MCPB is photodegraded with an ultraviolet (UV) lamp and the photoproducts formed provide a great CL signal when they react with ferricyanide in basic medium. Second, a high-performance liquid chromatography chemiluminescence (HPLC-CL) method is proposed. In this method, before the photodegradation and CL reaction, the MCPB and other phenoxyacid herbicides are separated in a C18 column. The experimental conditions for the FI-CL and HPLC-CL methods are optimized. Both methods present good sensitivity, the detection limits being 0.12 µg L(-1) and 0.1 µg L(-1) (for FI-CL and HPLC-CL, respectively) when solid phase extraction (SPE) is applied. Intra- and interday relative standard deviations are below 9.9%. The methods have been satisfactorily applied to the analysis of natural water samples. FI-CL method can be employed for the determination of MCPB in simple water samples and for the screening of complex water samples in a fast, economic, and simple way. The HPLC-CL method is more selective, and allows samples that have not been resolved with the FI-CL method to be solved.

  5. Inkjet nanoinjection for high-thoughput chemiluminescence immunoassay on multicapillary glass plate.

    Science.gov (United States)

    Chen, Fengming; Mao, Sifeng; Zeng, Hulie; Xue, Shuhua; Yang, Jianmin; Nakajima, Hizuru; Lin, Jin-Ming; Uchiyama, Katsumi

    2013-08-06

    We report a novel chemiluminescence diagnosis system for high-throughput human IgA detection by inkjet nanoinjection on a multicapillary glass plate. As proof-of-concept, microhole-based polydimethylsiloxane (PDMS) sheets were aligned on a multicapillary glass plate to form a microwell array as microreactors for enzyme-linked immunosorbent assay (ELISA). The multicapillary glass plate was utilized as a switch that controlled the holding/passing of the solution. Further, anti-IgA-labeled polystyrene (PS) microbeads was assembled into the microwell array, and an inkjet nanoinjection was specially used to distribute the sample and reagent solution for chemiluminescence ELISA, enabling high-throughput detection of human IgA. As a result, the performance of human IgA tests revealed a wider range for the calibration curve and a lower limit of detection (LOD) of 0.1 ng mL(-1) than the ELISA by a standard 96-well plate. The analysis time and reagent consumption were significantly decreased. The IgA concentrations in saliva samples were determined after 10000-fold dilution by the developed ELISA system showing comparable results by conventional immune assay with 96-wells. Thus, we believe that the inkjet nanoinjection for high-throughput chemiluminescence immunoassay on a multicapillary glass plate will be promising in disease diagnosis.

  6. Chemiluminescent detect of E. coli O157:H7 using immunological method based on magnetic nanoparticles.

    Science.gov (United States)

    Li, Zhiyang; He, Lei; He, Nongyue; Shi, Zhiyang; Wang, Hua; Li, Song; Liu, Hongna; Li, Xiaolong; Dai, Yabin; Wang, Zhifei

    2010-02-01

    The system of chemiluminescent magnetic enzyme-linked immunoassay was developed. E. coli O157:H7 was sandwiched between rabbits anti-E. coli O157:H7 polyclonal antibody-coated magnetic nanoparticles (immunomagnetic nanoparticles or IMNPs) and mouse anti-E. coli O157:H7 monoclonal antibody. Commercial alkaline phosphatase conjugated horse anti-mouse immunoglobulin (ALP-Ab) was used to bind with the monoclonal antibody, finally the chemiluminescent signals were detected by adding 3-(2'-spiroadamantane)-4-methoxy-4-(3"-phosphoryloxy)phenyl-1,2-dioxetane (AMPPD) which was the substrate reagent of ALP. Different solvents of AMPPD were compared to get an optimal chemiluminescent signal. The effects of sodium borohydride and glycine on blocking the aldehyde groups of IMNPs were compared either, and the specificity and sensitivity of this system for detecting E. coli O157:H7 were researched. The results indicated that Tris buffer was the best solvent of AMPPD, sodium borohydride was better than glycine in blocking IMNPs, and this method was of good specificity when using E. coli Top 10F' and Vibrio cholera as negative controls. The detection limit was 10(3) cells mL(-1) when the antigen solution was 1 mL, and the procedure duration was about 3 h.

  7. Schlieren and OH* chemiluminescence imaging of combustion in a turbulent boundary layer over a solid fuel

    Science.gov (United States)

    Jens, Elizabeth T.; Miller, Victor A.; Cantwell, Brian J.

    2016-03-01

    Combustion in a turbulent boundary layer over a solid fuel is studied using simultaneous schlieren and OH* chemiluminescence imaging. The flow configuration is representative of a hybrid rocket motor combustor. Six different hydrocarbon fuels, including both classical hybrid rocket fuels and a high regression rate fuel (paraffin wax), are burned in an undiluted oxygen free-stream at pressures ranging from atmospheric to 1524.2 kPa (221.1 psi). A detailed explanation of methods for registering the schlieren and OH* chemiluminescence images to one another is presented, and additionally, details of the routines used to extract flow features of interest (like the boundary layer height and flame location) are provided. At atmospheric pressure, the boundary layer location is consistent between all fuels; however, the flame location varies for each fuel. The flame zone appears to be smoothly distributed over the fuel surface at atmospheric pressure. At elevated pressures and correspondingly increased Dahmköhler number (but at constant Reynolds number), flame morphology is markedly different, exhibiting large rollers in a shear layer above the fuel grain and finer structures in the flame. The chemiluminescence intensity is found to be roughly proportional to the fuel burn rate at both atmospheric and elevated chamber pressures.

  8. Validation Procedure for Multiplex Antibiotic Immunoassays Using Flow-Based Chemiluminescence Microarrays.

    Science.gov (United States)

    Meyer, Verena Katharina; Meloni, Daniela; Olivo, Fabio; Märtlbauer, Erwin; Dietrich, Richard; Niessner, Reinhard; Seidel, Michael

    2017-01-01

    Small molecules like antibiotics or other pharmaceuticals can be detected and quantified, among others, with indirect competitive immunoassays. With regard to multiplex quantification, these tests can be performed as chemiluminescence microarray immunoassays, in which, in principle, the analyte in the sample and the same substance immobilized on the chip surface compete for a limited number of specific antibody binding sites. The amount of the specific primary antibody that has been bound to the surface is visualized by means of a chemiluminescence reaction.Validated quantitative confirmatory methods for the detection of contaminants, for example drug residues, in food samples usually comprise chromatographic analysis and spectrometric detection, e.g., HPLC-MS, GC-MS, or GC with electron capture detection. Here, we describe a validation procedure (according to the Commission Decision of the European Communities 2002/657/EC) for multiplex immunoassays performed as flow-through chemiluminescence microarrays, using the example of a small molecule microarray for the simultaneous detection of 13 antibiotics in milk. By this means, we suggest to accept multianalyte immunoassays as confirmatory methods as well, to benefit from the advantages of a fast automated method that does not need any pretreatment of the sample. The presented microarray chip is regenerable, so an internal calibration is implemented. Therefore, the analytical results are highly precise, combined with low costs (the aim for commercialization is less than 1 € per analyte per sample, this is significantly less than HPLC-MS).

  9. Development of ultrasensitive direct chemiluminescent enzyme immunoassay for determination of aflatoxin M1 in milk.

    Science.gov (United States)

    Vdovenko, Marina M; Lu, Chuan-Chen; Yu, Feng-Yih; Sakharov, Ivan Yu

    2014-09-01

    A direct competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for detecting aflatoxin M1 (AFM1) was developed. To improve the sensitivity of the assay, a mixture of 3-(10'-phenothiazinyl)-propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORPH) was used to enhance peroxidase-induced CL. The concentrations of the coating anti-AFM1 antibody and the conjugate of AFB1 with horseradish peroxidase the conditions of the chemiluminescent assay were varied to optimise the condition of the chemiluminescent assay. The lower detection limit values and dynamic working range of CL-ELISA of AFM1 were 0.001 ng mL(-1) and 0.002-0.0075 ng mL(-1), respectively. A 20-fold dilution of milk samples prevented a matrix effect of the milk and allowed measurement of AFM1 at concentrations below than the maximum acceptable limit. Values of recovery within and between assays were 81.5-117.6% and 86-110.6%, respectively. The results of using the developed CL-ELISA to analyse samples of six brands of milk that were purchased in Taiwan revealed that AFM1 was absent from all studied samples.

  10. Detection of synthetic corticosteroids in bovine urine by chemiluminescence high-performance liquid chromatography.

    Science.gov (United States)

    Vázquez, B I; Feás, X; Lolo, M; Fente, C A; Franco, C M; Cepeda, A

    2005-01-01

    The development of a black market of chemical cocktails for illegal growth promotion in food-producing animals includes substances that are potentially dangerous for human health, such as synthetic corticosteroids. The potential presence of these residues in food makes it necessary to develop rapid and sensitive analytical methodologies to detect such substances, preferably in live animals before they arrive at the market. A chemiluminescence (CL) detection method for the determination of four synthetic corticosteroids (prednisolone, betamethasone, dexamethasone and flumethasone) in bovine urine has been developed. The proposed system, which does not need any derivatization procedure, offers an easy method well suited for routine research. Urine samples were homogenized with methanol:water (50:50, v/v) and centrifuged. The upper layer was collected and Strata X cartridges were used for cleaning up. The purified residues were evaporated to dryness and then redissolved in the mobile phase. Analysis of the extracts was performed using high-performance liquid chromatography with chemiluminescence detection, employing luminol as the CL reagent. The recovery curves, obtained at four spiking levels (different for each corticosteroid), showed that recoveries of at least 70% could be obtained for urine. The chemiluminescence detection procedure afforded satisfactory results with respect to sensitivity and the LOD and LOQ, taken as the first point of the regression curve, ranged from 4 ppb to 65 ppb. The maximum mean RSD was below 13% and below 15% for intra- and inter-day assay, respectively, in all cases.

  11. A new method for immunoassays using field-flow fractionation with on-line, continuous chemiluminescence detection.

    Science.gov (United States)

    Melucci, D; Guardigli, M; Roda, B; Zattoni, A; Reschiglian, P; Roda, A

    2003-06-13

    Chemiluminescence detection has already been combined with different separation techniques such as HPLC and capillary electrophoresis. In this work, it was applied to gravitational field-flow fractionation, a low-cost, flow-assisted separation technique for micronsized particles suited to further on-line detection of the separated analytes. Horseradish peroxidase was used as model sample, either free in solution or immobilized onto micronsized, polystyrene beads. The chemiluminescent substrates were added directly into the mobile phase, and the continuous, steady-state chemiluminescence generated during elution was detected on-line by either a flow-through luminometer or a CCD camera. Ultra-low detection limits, two orders of magnitude lower than those achievable with spectrophotometric detection, were found. The possibility to fully separate and quantitate free and bead-immobilized enzymes is reported, as a step towards the development of multianalyte, ultra-sensitive, micronsized beads-based flow-assisted immunoassays.

  12. Flow-injection chemiluminescence determination of acetylsalicylic acid based on its enhancing effect on the lucigenin–hydrogen peroxide system.

    Science.gov (United States)

    Wabaidur, S M; Alam, S M; Alothmana, Z A; Eldesokya, Gaber

    2014-09-01

    A sensitive flow-injection chemiluminescence method for the determination of acetylsalicylic acid is described. It is based on the enhanced chemiluminescent emission of the alkaline lucigenin–H2O2 system by acetylsalicylic acid. The difference in chemiluminescent intensity of alkaline lucigenin–H2O2 in the presence of acetylsalicylic acid from that in the absence of acetylsalicylic acid was linear at acetylsalicylic acid concentrations in the range of 0.0029–47.37 μg/mL, with detection and quantification limits of 0.0011 and 0.0029 μg/mL, respectively. The correlation coefficient of the working curve was 0.9983. The relative standard deviation (n = 10) for 25 μg/mL acetylsalicylic acid is 1.95%. All experimental parameters were optimized. The method was successfully applied to the determination of acetylsalicylic acid in pharmaceutical preparations. The recovery results obtained by the method were satisfactory.

  13. Automated determinations of selenium in thermal power plant wastewater by sequential hydride generation and chemiluminescence detection.

    Science.gov (United States)

    Ezoe, Kentaro; Ohyama, Seiichi; Hashem, Md Abul; Ohira, Shin-Ichi; Toda, Kei

    2016-02-01

    After the Fukushima disaster, power generation from nuclear power plants in Japan was completely stopped and old coal-based power plants were re-commissioned to compensate for the decrease in power generation capacity. Although coal is a relatively inexpensive fuel for power generation, it contains high levels (mgkg(-1)) of selenium, which could contaminate the wastewater from thermal power plants. In this work, an automated selenium monitoring system was developed based on sequential hydride generation and chemiluminescence detection. This method could be applied to control of wastewater contamination. In this method, selenium is vaporized as H2Se, which reacts with ozone to produce chemiluminescence. However, interference from arsenic is of concern because the ozone-induced chemiluminescence intensity of H2Se is much lower than that of AsH3. This problem was successfully addressed by vaporizing arsenic and selenium individually in a sequential procedure using a syringe pump equipped with an eight-port selection valve and hot and cold reactors. Oxidative decomposition of organoselenium compounds and pre-reduction of the selenium were performed in the hot reactor, and vapor generation of arsenic and selenium were performed separately in the cold reactor. Sample transfers between the reactors were carried out by a pneumatic air operation by switching with three-way solenoid valves. The detection limit for selenium was 0.008 mg L(-1) and calibration curve was linear up to 1.0 mg L(-1), which provided suitable performance for controlling selenium in wastewater to around the allowable limit (0.1 mg L(-1)). This system consumes few chemicals and is stable for more than a month without any maintenance. Wastewater samples from thermal power plants were collected, and data obtained by the proposed method were compared with those from batchwise water treatment followed by hydride generation-atomic fluorescence spectrometry.

  14. Evaluation of nitrogen dioxide chemiluminescence monitors in a polluted urban environment

    Directory of Open Access Journals (Sweden)

    M. J. Molina

    2007-05-01

    Full Text Available Data from a recent field campaign in Mexico City are used to evaluate the performance of the EPA Federal Reference Method for monitoring the ambient concentrations of NO2. Measurements of NO2 from standard chemiluminescence monitors equipped with molybdenum oxide converters are compared with those from Tunable Infrared Laser Differential Absorption Spectroscopy (TILDAS and Differential Optical Absorption Spectroscopy (DOAS instruments. A significant interference in the chemiluminescence measurement is shown to account for up to 50% of ambient NO2 concentration during afternoon hours. As expected, this interference correlates well with non-NOx reactive nitrogen species (NOz as well as with ambient O3 concentrations, indicating a photochemical source for the interfering species. A combination of ambient gas phase nitric acid and alkyl and multifunctional alkyl nitrates is deduced to be the primary cause of the interference. Observations at four locations at varying proximities to emission sources indicate that the percentage contribution of HNO3 to the interference decreases with time as the air parcel ages. Alkyl and multifunctional alkyl nitrate concentrations are calculated to reach concentrations as high as several ppb inside the city, on par with the highest values previously observed in other urban locations. Averaged over the MCMA-2003 field campaign, the chemiluminescence monitor interference resulted in an average measured NO2 concentration up to 22% greater than that from co-located spectroscopic measurements. Thus, this interference has the potential to initiate regulatory action in areas that are close to non-attainment and may mislead atmospheric photochemical models used to assess control strategies for photochemical oxidants.

  15. Chemiluminescence Determination of Molybdenum by on-Line Reduction with a Flow Injection System

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The chemiluminescence (CL) reaction between lucigenin (Lu) and molybdenum (Ⅲ) produced by a Jones reductor was investigated using a flow injection system. On the basis of this, a novel method for the determination of trace amount of molybdenum has been established. The emission intensity was linear with molybdenum concentration in the range of 0.1-1000 ng/mL; the detection limit was 0.02 ng/mL molybdenum; the relative standard deviation was less than 2% for the determination of 0.1 ng/mL molybdenum (n=11). The method has been applied successfully to the analysis of trace molybdenum in water and steel samples.

  16. Chemiluminescence of Organic Peroxides. Thermal Generation of an o-Xylylene Peroxide.

    Science.gov (United States)

    1981-04-07

    Our efforts to discover new chemiluminescent systems and to probe the mechanism of electronically excited state generation led to the synthesis and...product is a precursor to diketone or ketoester k we carried out the thermolysis of in the presence of maleic anhydride. A 1.Ox]O - 3 I p-xylene solution of... diketone -3- that is essentially the same as in the absence of anhydride. Increasing the maleic anhydride concentration to 1.5x10- 2 M results in the

  17. Recent advances in chemiluminescence detection coupled with capillary electrophoresis and microchip capillary electrophoresis.

    Science.gov (United States)

    Liu, Yuxuan; Huang, Xiangyi; Ren, Jicun

    2016-01-01

    CE is an ideal analytical method for extremely volume-limited biological microenvironments. However, the small injection volume makes it a challenge to achieve highly sensitive detection. Chemiluminescence (CL) detection is characterized by providing low background with excellent sensitivity because of requiring no light source. The coupling of CL with CE and MCE has become a powerful analytical method. So far, this method has been widely applied to chemical analysis, bioassay, drug analysis, and environment analysis. In this review, we first introduce some developments for CE-CL and MCE-CL systems, and then put the emphasis on the applications in the last 10 years. Finally, we discuss the future prospects.

  18. Flow Injection Analysis of Histidine with Enhanced Electrogenerated Chemiluminescence of Luminol

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A simple and sensitive flow injection method is presented for the determination of histidine based on its enhancement of electrogenerated chemiluminescence (ECL) of luminol. After optimization of the experimental parameters, the working range for histidine was in 1.0 x 10-6 to 1.0 x 10 -3 mol/L with a detection limit (S/N = 3) of 0.56 μmol/L. The relative standard deviation was 1.6% for 11 measurements of 5 x 10 -5 mol/L histidine solution. The proposed method has been successfully applied to the determination of histidine in real pharmaceutical preparation.

  19. Determination of Baicalin in Traditional Chinese Preparation by High Performance Liquid Chromatography with Chemiluminescence Detection

    Institute of Scientific and Technical Information of China (English)

    Er Bao LIU; Hong Qing WEI; Xiu Li ZHAO; Xiao Xia LI; Feng Xian JIANG

    2004-01-01

    A new method for the determination of baicalin with HPLC-CL was developed. The method was based on the chemiluminescence reaction between KMnO4 and baicalin sensitized from HCHO. The linear range was 3.7×10-6~9.8×10-5 mol/L with detection limit of 1.7×10-6 mol/L and the relative standard deviation was 2.5 % (Cs=6.6×10-5 mol/L, n=5). The method has been applied to the determination of baicalin in oral administration, injection, Scutellariae radix and granules with good results.

  20. Chemiluminescence detection of organic peroxides in a two-phase system.

    Science.gov (United States)

    Baj, Stefan; Krawczyk, Tomasz

    2007-02-28

    Potential application of chemiluminescence (CL) reaction of luminol for detection of organic peroxides by HPLC post-column reaction using an immiscible apolar eluent (hexane) with aqueous solution of luminol has been proposed. The positive influence of the addition of an anionic surface-active agent--sodium dodecyl sulphate (SDS) to the luminol solution on the CL intensity has been observed. The sensitivity of the method is greater for peroxyacid and hydroperoxide tested, and lesser for diacyl peroxide as compared to system with polar eluent miscible with solution of luminol. Our interpretation of observed results based on the extraction efficiency, CL kinetics and microemulsion formation has been suggested.

  1. Chemiluminescence of phthalhydrazide derivatives in organized media: Interactions with surfactants and cyclodextrins

    Energy Technology Data Exchange (ETDEWEB)

    Maeztu, Raquel [Departamento de Quimica y Edafologia, Facultad de Ciencias, Universidad de Navarra, 31080 Pamplona (Spain); Gonzalez-Gaitano, Gustavo, E-mail: gaitano@unav.e [Departamento de Quimica y Edafologia, Facultad de Ciencias, Universidad de Navarra, 31080 Pamplona (Spain); Tardajos, Gloria [Departamento de Quimica-Fisica I, Facultad de CC. Quimicas, Universidad Complutense, 28040 Madrid (Spain); Stilbs, Peter [Division of Physical Chemistry and Industrial NMR Centre, Department of Chemistry, Royal Institute of Technology, SE-10044, Stockholm (Sweden)

    2011-04-15

    The chemiluminescent oxidation of some phthalhydrazide derivatives, luminol (LUM), isoluminol (ISOL), N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and N-(6-aminohexyl)-N-ethylisoluminol (AHEI), has been carried out in micellar media and in the presence of natural cyclodextrins (CDs), using Co(II) as catalyst and H{sub 2}O{sub 2} as oxidant. The cationic cetyltrimethylammonium bromide (CTAB) and, in a lesser extent, the anionic sodium dodecyl sulfate (SDS) produce quenching in the chemiluminescence (CL) of all the luminophores at concentrations above the cmc, whereas slight enhancements are attained with the non-ionic pentaethylene glycol monododecyl ether (C{sub 12}E{sub 5}). On the contrary, the incorporation of the CDs to the reaction produces a remarkable intensification of the CL. Binding of the luminophores to the macrocycles and the micelles has been studied by Pulsed-Gradient-Spin-Echo-NMR (PGSE-NMR) and fluorescence anisotropy. The cationic CTAB decreases the emission mainly due to charge compensation as a result of the association of the luminophores and the luminescent intermediates to the micelles. The presence of the alkyl substituents of ABEI and AHEI provides an additional hydrophobic contribution to the binding process. SDS quenches this reaction as the micelles retain Co(II) on their anionic layer. The protection and stabilization of these luminophores or their luminescent intermediates provided by the cavities of the CDs make this family of cyclic oligosaccharides much more suitable agents than the surfactants for enhancing the CL in aqueous media for this specific reaction. - Research highlights: Natural cyclodextrins act as boosters of the chemiluminescence of luminol and its derivatives. Cyclodextrins can be successfully employed for improving the possibilities of those applications that make use of the CL of luminol and its derivatives as a detection or quantification method. Ionic and non-ionic surfactants do not either enhance or even quench the

  2. Chemiluminescence determination of melamine with Luminol-K3Fe(CN)6 system%Chemiluminescence determination of melamine with Luminol-K3Fe(CN)6 system

    Institute of Scientific and Technical Information of China (English)

    Xiao- Shuang Tang; Xi-Yan Shi; Yu-Hai Tang; Zhong-Jin Yue; Qi-Qi He

    2011-01-01

    A sensitive chemiluminescence(CL) method was developed for determining melamine in urine and plasma samples based on the fact that melamine can remarkably enhance the chemiluminescence of Luminol-K3 Fe(CN)6 system in alkaline medium. The determination con

  3. Determination of Biotin in Pharmaceutical Formulations by Potassium Permanganate-luminol-CdTe Nanoparticles Chemiluminescence System

    Institute of Scientific and Technical Information of China (English)

    TRAORE Zoumana Sékou; SU Xing-guang

    2012-01-01

    A sensitive flow-injection chemiluminescence method was developed for the determination of biotin in the pharmaceutical formulations.The affinity between avidin and biotin was used to adsorb biotin on the polystyrene,with subsequent quantification of biotin based on its ability to enhance the chemiluminescence(CL) signal generated by the redox reaction of potassium permanganate-luminol-CdTe nanoparticles CL system.The investigations prove that apart from 3-aminophthalate,the CdTe quantum dots(QDs) play both catalytic and emitter roles.Under optimum conditions,the linear range for the determination of biotin was 0.01-25 ng/mL with a detection limit of 7.3×10-3ng/mL(S/N=3).The relative standard deviation of 5 ng/L biotin was 2.06%(n=7).The proposed method was used to determine the biotin concentration in the pharmaceutical formulations and the recovery was between 96.4% and 104%.The proposed method is simple,convenient,rapid and sensitive.

  4. A new sensitive flow-injection chemiluminescence method for the determination of H(2)-receptor antagonists.

    Science.gov (United States)

    Tang, Yu-Hai; Wang, Nan-Nan; Xiong, Xun-Yu; Xiong, Feng-Mei; Sun, Si-Juan

    2007-01-01

    Based on the chemiluminescence (CL) intensity generated from the potassium ferricyanide [K(3)Fe(CN)(6)]-rhodamine 6G system in sodium hydroxide (NaOH) medium, a new sensitive flow-injection chemiluminescence (FI-CL) method has been developed, validated and applied for the determination of three kinds of H(2)-receptor antagonists: cimetidine (CIMT), ranitidine (RANT) hydrochloride and famotidine (FAMT). Under the optimum conditions, the linear range for the determination was 1.0 x 10(-9)-7.0 x 10(-5) g/ml for CIMT, 1.0 x 10(-9)-5.0 x 10(-5) g/mL for RANT hydrochloride and 5.0 x 10(-9)-7.0 x 10(-5) g/mL for FAMT. During 11 repeated measurements of 1.0 x 10(-6) g/mL sample solutions, the relative standard deviations (RSDs) were all <5%. The detection limit was 8.56 x 10(-10) g/mL for CIMT, 8.69 x 10(-10) g/mL for RANT hydrochloride and 2.35 x 10(-9) g/mL for FAMT (S:N = 3). This method has been successfully implemented for the analysis of H(2)-receptor antagonists in pharmaceuticals.

  5. Correlation between arachidonic acid oxygenation and luminol-induced chemiluminescence in neutrophils: inhibition by diethyldithiocarbamate.

    Science.gov (United States)

    Chabannes, B; Perraut, C; El Habib, R; Moliere, P; Pacheco, Y; Lagarde, M

    1997-04-01

    Neutrophils from allergic subjects were hypersensitive to stimulation by low calcium ionophore concentration (0.15 microM), resulting in an increased formation of leukotriene B4 (LTB4), 5S-hydroxy-6,8,11,14-(E,Z,Z,Z)-eicosatetraenoic acid (5-HETE), and other arachidonic acid metabolites through the 5-lipoxygenase pathway. In parallel, luminol-dependent chemiluminescence was also higher in neutrophils from allergic patients at the basal state and after stimulation by calcium ionophore, revealing an enhancement of radical oxygen species and peroxide production. The activity of glutathione peroxidase, the main enzyme responsible for hydroperoxide reduction, was lowered in these cells. Diethyl-dithiocarbamate (DTC) induced a concentration-dependent decrease in chemiluminescence and arachidonic acid metabolism after neutrophil stimulation. These data show that the elevation of arachidonic acid metabolism in neutrophils from allergic patients is strongly correlated with oxidative status. This elevation may be the consequence of an increased cellular hydroperoxide known to activate 5-lipoxygenase (5-LOX) activity and/or an increased arachidonic acid availability, due either to phospholipase A2 (PLA2) activation or inhibition of arachidonate reesterification into phospholipids. Lowering this oxidative status was associated with a concomitant decrease of this metabolism. Our results suggest that the effect of DTC may be the consequence of an inhibition of peroxyl radical and cellular lipid hydroperoxide production. Thus, DTC may modulate arachidonic acid metabolism in neutrophils by modulating the cellular hydroperoxide level.

  6. Evaluation of Antibacterial Enrofloxacin in Eggs by Matrix Solid Phase Dispersion-Flow Injection Chemiluminescence

    Directory of Open Access Journals (Sweden)

    Xiaocui Duan

    2014-01-01

    Full Text Available The study based on the chemiluminescence (CL reaction of potassium ferricyanide and luminol in sodium hydroxide medium, enrofloxacin (ENRO could dramatically enhance CL intensities and incorporated with matrix solid-phase dispersion (MSPD technique (Florisil used as dispersant, dichloromethane eluted the target compounds. A simple flow injection chemiluminescence (FL-CL method with MSPD technique for determination of ENRO in eggs was described. Under optimal conditions, the CL intensities were linearly related to ENRO concentration ranging from 4.0×10-8 g.L−1 to 5.0×10-5 g.L−1, with a correlation coefficient of 0.9989 and detection limit of 5.0×10-9 g.L−1. The relative standard deviation was 3.6% at an ENRO concentration of 2.0×10-6 g.L−1. Our testing technique can help ensure food safety, and thus, protect public health.

  7. The observation of chemiluminescent NiO* emissions in the laboratory and in the night airglow

    Directory of Open Access Journals (Sweden)

    A. L. Broadfoot

    2011-04-01

    Full Text Available The recent finding of an orange spectral feature in OSIRIS/Odin spectra of the night airglow near 85 km has raised interest in the origin of the emission. The feature was positively identified as the chemiluminescent FeO* emission where the iron is of meteoric origin. Since the meteorite source of atomic metals in the mesosphere contains both iron and nickel, with Ni being typically 6% of Fe, it is expected that faint emissions involving Ni should also be present in the night airglow. The present study summarizes the laboratory observations of chemiluminescent NiO* emissions and includes a search for the NiO* signature in the night airglow. A faint previously unidentified "continuum" extending longwave of 440 nm has been identified in night airglow spectra obtained with two space-borne limb viewing instruments and through a comparison with laboratory spectra this continuum is identified as arising from the NiO* emission. The FeO* and NiO* emissions both originate from a reaction of the metal atoms with mesospheric ozone and so support the presence of NiO* in the night airglow.

  8. The observation of chemiluminescent NiO* emissions in the laboratory and in the night airglow

    Directory of Open Access Journals (Sweden)

    W. F. J. Evans

    2011-09-01

    Full Text Available The recent finding of an orange spectral feature in OSIRIS/Odin spectra of the night airglow near 87 km has raised interest in the origin of the emission. The feature was positively identified as the chemiluminescent FeO* emission where the iron is of meteoric origin. Since the meteorite source of atomic metals in the mesosphere contains both iron and nickel, with Ni being typically 6 % of Fe, it is expected that faint emissions involving Ni should also be present in the night airglow. The present study summarizes the laboratory observations of chemiluminescent NiO* emissions and includes a search for the NiO* signature in the night airglow. A very faint previously unidentified "continuum" extending longwave of 440 nm has been detected in the night airglow spectra obtained with two space-borne limb viewing instruments. Through a comparison with laboratory spectra this continuum is identified as arising from the NiO* emission. The altitude profile of the new airglow emission has also been measured. The similarity of the altitude profiles of the FeO* and NiO* emissions also suggests the emission is NiO as both can originate from reaction of the metal atoms with mesospheric ozone. The observed NiO* to FeO* ratio exhibits considerable variability; possible causes of this observed variation are briefly discussed.

  9. Microplate-reader method for the rapid analysis of copper in natural waters with chemiluminescence detection

    Directory of Open Access Journals (Sweden)

    Axel eDurand

    2013-01-01

    Full Text Available We have developed a method for the determination of copper in natural waters at nanomolar levels. The use of a microplate-reader minimises sample processing time (~ 25 sec per sample, reagent consumption (~ 120 μL per sample and sample volume (~ 700 μL. Copper is detected by chemiluminescence. This technique is based on the formation of a complex between copper and 1,10-phenanthroline and the subsequent emission of light during the oxidation of the complex by hydrogen peroxide. Samples are acidified to pH 1.7 and then introduced directly into a 24-well plate. Reagents are added during data acquisition via two reagent injectors. When trace metal clean protocols are employed, the reproducibility is generally less then 7% on blanks and the detection limit is 0.7 nM for seawater and 0.4 nM for freshwater. More than 100 samples per hour can be analyzed with this technique, which is simple, robust, and amenable to at-sea analysis. Seawater samples from Storm Bay in Tasmania illustrate the utility of the method for environmental science. Indeed other trace metals for which optical detection methods exist (e.g. chemiluminescence, fluorescence and absorbance could be adapted to the microplate-reader.

  10. Exhaled nitric oxide monitoring by quantum cascade laser: comparison with chemiluminescent and electrochemical sensors.

    Science.gov (United States)

    Mandon, Julien; Högman, Marieann; Merkus, Peter J F M; van Amsterdam, Jan; Harren, Frans J M; Cristescu, Simona M

    2012-01-01

    Fractional exhaled nitric oxide (F(E)NO) is considered an indicator in the diagnostics and management of asthma. In this study we present a laser-based sensor for measuring F(E)NO. It consists of a quantum cascade laser (QCL) combined with a multi-pass cell and wavelength modulation spectroscopy for the detection of NO at the sub-part-per-billion by volume (ppbv, 110(-9)) level. The characteristics and diagnostic performance of the sensor were assessed. A detection limit of 0.5 ppbv was demonstrated with a relatively simple design. The QCL-based sensor was compared with two market sensors, a chemiluminescent analyzer (NOA 280, Sievers) and a portable hand-held electrochemical analyzer (MINO, Aerocrine AB, Sweden). F(E)NO from 20 children diagnosed with asthma and treated with inhaled corticosteroids were measured. Data were found to be clinically acceptable within 1.1 ppbv between the QCL-based sensor and chemiluminescent sensor and within 1.7 ppbv when compared to the electrochemical sensor. The QCL-based sensor was tested on healthy subjects at various expiratory flow rates for both online and offline sampling procedures. The extended NO parameters, i.e. the alveolar region, airway wall, diffusing capacity, and flux were calculated and showed a good agreement with the previously reported values.

  11. Detection of ochratoxin A (OTA) in coffee using chemiluminescence resonance energy transfer (CRET) aptasensor.

    Science.gov (United States)

    Jo, Eun-Jung; Mun, Hyoyoung; Kim, Su-Ji; Shim, Won-Bo; Kim, Min-Gon

    2016-03-01

    We report a chemiluminescence resonance energy transfer (CRET) aptasensor for the detection of ochratoxin A (OTA) in roasted coffee beans. The aptamer sequences used in this study are 5'-DNAzyme-Linker-OTA aptamer-3'-dabcyl. Dabcyl at the end of the OTA aptamer region plays as a quencher in CRET aptasensor. When hemin and OTA are added, the dabcyl-labeled OTA aptamer approaches to the G-quadruplex-hemin complex by formation of the G-quadruplex-OTA complex. The G-quadruplex-hemin complexes possess horseradish peroxidase (HRP)-like activity, and therefore, the HRP-mimicking DNAzyme (HRPzyme) catalyzes peroxidation in the presence of luminol and H2O2. Resonance energy transfer between luminol (donor) and dabcyl (acceptor) enables quenching of chemiluminescence signals. The signal decreases with increasing the concentration of OTA within the range of 0.1-100ngmL(-1) (limit of detection 0.22ngmL(-1)), and the level of recovery of the respective 1ngmL(-1) and 10ngmL(-1) spiked coffee samples was 71.5% and 93.3%. These results demonstrated the potential of the proposed method for OTA analysis in diverse foods.

  12. Exhaled nitric oxide monitoring by quantum cascade laser: comparison with chemiluminescent and electrochemical sensors

    Science.gov (United States)

    Mandon, Julien; Högman, Marieann; Merkus, Peter J. F. M.; van Amsterdam, Jan; Harren, Frans J. M.; Cristescu, Simona M.

    2012-01-01

    Fractional exhaled nitric oxide (FENO) is considered an indicator in the diagnostics and management of asthma. In this study we present a laser-based sensor for measuring FENO. It consists of a quantum cascade laser (QCL) combined with a multi-pass cell and wavelength modulation spectroscopy for the detection of NO at the sub-part-per-billion by volume (ppbv, 1∶10-9) level. The characteristics and diagnostic performance of the sensor were assessed. A detection limit of 0.5 ppbv was demonstrated with a relatively simple design. The QCL-based sensor was compared with two market sensors, a chemiluminescent analyzer (NOA 280, Sievers) and a portable hand-held electrochemical analyzer (MINO®, Aerocrine AB, Sweden). FENO from 20 children diagnosed with asthma and treated with inhaled corticosteroids were measured. Data were found to be clinically acceptable within 1.1 ppbv between the QCL-based sensor and chemiluminescent sensor and within 1.7 ppbv when compared to the electrochemical sensor. The QCL-based sensor was tested on healthy subjects at various expiratory flow rates for both online and offline sampling procedures. The extended NO parameters, i.e. the alveolar region, airway wall, diffusing capacity, and flux were calculated and showed a good agreement with the previously reported values.

  13. A chemiluminescent metalloimmunoassay based on silver deposition on colloidal gold labels.

    Science.gov (United States)

    Li, Zheng-Ping; Liu, Cheng-Hui; Fan, Yong-Shan; Wang, Yu-Cong; Duan, Xin-Rui

    2006-12-15

    A sensitive chemiluminescent (CL) immunoassay of human immunoglobulin (IgG) which combined the inherent high sensitivity of CL analysis with the dramatic signal amplification of silver precipitation on colloidal gold tags was developed. First, the sandwich-type complex was formed in this protocol by the primary antibody immobilized on the polystyrene wells, the analyte in the sample, and the secondary antibody labeled with colloidal gold. Second, the colloidal gold was treated by an Ag(+) reduction solution, which resulted in the catalytic precipitation of silver on the surface of colloidal gold. Third, a large number of Ag(+) were oxidatively released in HNO(3) solution from the silver metal anchored on the sandwich-type complexes and then the human IgG was indirectly determined by a sensitive combined CL reaction of Ag(+)-K(2)S(2)O(8)-Mn(2+)- H(3)PO(4)-luminol. The chemiluminescence intensity depends linearly on the logarithm of the concentration of human IgG over the range of 0.02-50ngml(-1) and detection limit (3sigma) is 0.005ngml(-1) (i.e., approximately 3x10(-14)M, 3amol in 100-mul sample). This assay has been successfully applied to the determination of human IgG in human serum samples and showed great potential for numerous applications in immunoassay.

  14. High throughput heme assay by detection of chemiluminescence of reconstituted horseradish peroxidase.

    Science.gov (United States)

    Takahashi, Shigekazu; Masuda, Tatsuru

    2009-06-01

    In living organisms, heme is an essential molecule for various biological functions. Recent studies also suggest that heme functions as organelle-derived signal that regulates fundamental cell processes. Furthermore, estimation of heme is widely used for studying various blood disorders. In this regard, development of a rapid, sensitive, and high throughput heme assay has been sought. The most frequently used method of measuring heme by pyridine hemochrome is time, labor, and material intensive, and therefore limiting in its utility for large scale, high throughput analysis. Recently, we reported alternative method that is sensitive and specific to heme, which is based on the ability of horseradish peroxidase (HRP) apo-enzyme to reconstitute with heme to form an active holo-enzyme. Here, we developed high throughput heme assay by performing reactions on multi-well plate with highly sensitive chemiluminescence detection reagents. Detection of chemiluminescence in charged coupled device (CCD)-based gel doc apparatus enables simultaneous measurement of multiple samples. Furthermore, the high sensitivity of this assay allowed a direct measurement of heme in solvent extracts after dilution. This assay is sensitive, quick, provides a large dynamic range, and is well suited for large-scale analysis of heme extracted from minute amount of samples.

  15. l-Tyrosine Contained in Dietary Supplement by Chemiluminescence Reaction of an Iron-Phthalocyanine Complex

    Directory of Open Access Journals (Sweden)

    Takao Ohtomo

    2012-01-01

    Full Text Available The chemiluminescence (CL signal immediately appeared when a hydrogen peroxide solution was injected into an iron-phthalocyanine tetrasulfonic acid (Fe-PTS aqueous solution. Moreover, the CL intensity of Fe-PTS decreased by adding L-tyrosine. Based on these results, the determination of trace amounts of L-tyrosine was developed using the quenching-chemiluminescence. The calibration curve of L-tyrosine was obtained in the concentration range of 2.0×10−7 M to 2.0×10−5 M. Moreover, the relative standard deviation (RSD was 1.63 % (=5 for 2.0×10−6 M L-tyrosine, and its detection limits (3σ were 1.81×10−7 M. The spike and recovery experiments for L-tyrosine were performed using a soft drink. Furthermore, the determination of L-tyrosine was applied to supplements containing various kinds of amino acids. Each satisfactory relative recovery was obtained at 98 to 102%.

  16. A comparison of chemiluminescent acridinium dimethylphenyl ester labels with different conjugation sites.

    Science.gov (United States)

    Natrajan, Anand; Wen, David

    2015-03-07

    Chemiluminescent acridinium dimethylphenyl esters are highly sensitive labels that are used in automated assays for clinical diagnosis. Light emission from these labels and their conjugates is triggered by treatment with alkaline peroxide. Conjugation of acridinium ester labels is normally done at the phenol. During the chemiluminescent reaction of these acridinium esters, the phenolic ester is cleaved and the light emitting acridone moiety is liberated from its conjugate partner. In the current study, we report the synthesis of three new acridinium esters with conjugation sites at the acridinium nitrogen and compare their properties with that of a conventional acridinium ester with a conjugation site at the phenol. Our study is the first that provides a direct comparison of the emissive properties of acridinium dimethylphenyl esters (free labels and protein conjugates) with different conjugation sites, one where the light emitting acridone remains attached to its conjugate partner versus conventional labeling which results in cleavage of the acridone from the conjugate. Our results indicate that the conjugation at the acridinium nitrogen, which also alters how the acridinium ring and phenol are oriented with respect to the protein surface, has a minimal impact on emission kinetics and emission spectra. However, this mode of conjugation to three different proteins led to a significant increase in light yield which should be useful for improving the assay sensitivity.

  17. Utility of gold nanoparticles in luminescence determination of trovafloxacin: comparison of chemiluminescence and fluorescence detection.

    Science.gov (United States)

    Alarfaj, Nawal A; El-Tohamy, Maha F

    2015-12-01

    Two novel sensitive sequential injection chemiluminescence analysis and fluorescence methods for trovafloxacin mesylate detection have been developed. The methods were based on the enhancement effect of gold nanoparticles on luminol-ferricyanide-trovafloxacin and europium(III)-trovafloxacin complex systems. The optimum conditions for both detection methods were investigated. The chemiluminescence signal was emitted due to the enhanced effect of gold nanoparticles on the reaction of luminol-ferricyanide-trovafloxacin in an alkaline medium. The response was linear over a concentration range of 1.0 × 10(-9) to 1.0 × 10(-2) mol/L (%RSD = 1.3), (n = 9, r = 0.9991) with a detection limit of 1.7 × 10(-10) mol/L (S/N = 3). The weak fluorescence intensity signal of the oxidation complex of europium(III)-trovafloxacin was strongly enhanced by gold nanoparticles and detected at λex = 330 and λem = 540 nm. Fluorescence detection enabled the determination of trovafloxacin mesylate over a linear range of 1.0 × 10(-8) to 1.0 × 10(-3) mol/L (%RSD = 1.2), (n = 6, r = 0.9993) with a detection limit of 3.3 × 10(-9) mol/L. The proposed methods were successfully applied to the determination of the studied drug in its bulk form and in pharmaceutical preparations. The results were treated statistically and compared with those obtained from other reported methods.

  18. CdS/MoS2 heterojunction-based photoelectrochemical DNA biosensor via enhanced chemiluminescence excitation.

    Science.gov (United States)

    Zang, Yang; Lei, Jianping; Hao, Qing; Ju, Huangxian

    2016-03-15

    This work developed a CdS/MoS2 heterojunction-based photoelectrochemical biosensor for sensitive detection of DNA under the enhanced chemiluminescence excitation of luminol catalyzed by hemin-DNA complex. The CdS/MoS2 photocathode was prepared by the stepwise assembly of MoS2 and CdS quantum dots (QDs) on indium tin oxide (ITO), and achieved about 280% increasing of photocurrent compared to pure CdS QDs electrode due to the formation of heterostructure. High photoconversion efficiency in the photoelectrochemical system was identified to be the rapid spatial charge separation of electron-hole pairs by the extension of electron transport time and electron lifetime. In the presence of target DNA, the catalytic hairpin assembly was triggered, and simultaneously the dual hemin-labeled DNA probe was introduced to capture DNA/CdS/MoS2 modified ITO electrode. Thus the chemiluminescence emission of luminol was enhanced via hemin-induced mimetic catalysis, leading to the physical light-free photoelectrochemical strategy. Under optimized conditions, the resulting photoelectrode was proportional to the logarithm of target DNA concentration in the range from 1 fM to 100 pM with a detection limit of 0.39 fM. Moreover, the cascade amplification biosensor demonstrated high selectivity, desirable stability and good reproducibility, showing great prospect in molecular diagnosis and bioanalysis.

  19. Investigation of RuBPS-Ce(IV) chemiluminescence reaction and its application in determination of two diuretics

    Energy Technology Data Exchange (ETDEWEB)

    Xi Juan [College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China); Ji Xinghu [College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China); Zhang Shaohong [College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China); Xiang Fan Vocational and Technical College, Xiangfan 441021 (China); Ai Xinping [College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China); He Zhike [College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China)]. E-mail: zhkhe@whu.edu.cn

    2005-06-13

    The chemiluminescence mechanism of tris-(4,7-diphenyl-1,10-phenanthrolinedisulfonic acid)ruthenium(II) (RuBPS)-Ce(IV) system and the effects of two diuretics, hydrochlorothiazide and furosemide, on its chemiluminescence intensity were investigated in detail. It was found that each of the two diuretics could enhance the chemiluminescence emission intensity of RuBPS-Ce(IV) system, based on which, they were sensitively detected by chemiluminescence analysis, respectively. Under the optimum experimental conditions, the linear range and detection limit of hydrochlorothiazide were 2.5 x 10{sup -3} to 6.0 x 10{sup -1} {mu}g ml{sup -1} and 1.0 x 10{sup -3} {mu}g ml{sup -1}, respectively; those of furosemide were 1.0 x 10{sup -2} to 4.0 {mu}g ml{sup -1} and 8.8 x 10{sup -3} {mu}g ml{sup -1}, respectively. The proposed method has been applied to analyze the pharmaceuticals with satisfied results.

  20. Simultaneous determination of chromium(III) and chromium(VI) in aqueous solutions by ion chromatography and chemiluminescence detection

    DEFF Research Database (Denmark)

    Gammelgaard, Bente; Jøns, O; Nielsen, B

    1992-01-01

    A method for the simultaneous determination of chromium(iii) and chromium(vi) in a flow system based on chemiluminescence was developed. A Dionex cation-exchange guard column was used to separate chromium(iii) from chromium(vi), and chromium(vi) was reduced by potassium sulfite, whereupon both...

  1. Enhanced sensitivity of Cypridina luciferin analog (CLA) chemiluminescence for the detection of O2- with non ionic detergents

    NARCIS (Netherlands)

    Osman, A.M.; Laane, C.; Hilhorst, R.

    2000-01-01

    Superoxide anion-triggered chemiluminescence of Cypridina luciferin analogue (CLA), 2-methyl-6-phenyl-3,7-dohydroimidazo[1,2-]pyrazin-3-one, is enhanced by non-ionic detergents such as Tween 20, Triton X-100 and Tween 80. At the concentration of 0.6øv/v) the largest increase (2.7-fold) of CLA light

  2. Advantages of soybean peroxidase over horseradish peroxidase as the enzyme label in chemiluminescent enzyme-linked immunosorbent assay of sulfamethoxypyridazine.

    Science.gov (United States)

    Sakharov, Ivan Yu; Berlina, Anna N; Zherdev, Anatoly V; Dzantiev, Boris B

    2010-03-24

    An indirect competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) of sulfamethoxypyridazine (SMP) was developed. The conjugates of streptavidin with cationic horseradish peroxidase (HRP) and anionic soybean peroxidase (SbP) were used in CL-ELISA for the detection of biotinylated anti-SMP antibodies. For streptavidin-HRP conjugate-catalyzed chemiluminescence measured 20 s after the initiation of the enhanced chemiluminescence reaction (ECR), the limit of detection (IC(10)), the IC(50) value, and the working range in CL-ELISA of SMP are 0.3, 12.4, and 1.2-85.0 ng/mL, respectively. An increase in the time interval between the ECR initiation and the luminescence measurement results in the loss in the quality of analytical measurements because of the time-dependent quenching of chemiluminescence typical of the HRP-catalyzed ECR. In the case of SbP-based CL-ELISA of SMP, the limit of detection, the IC(50) value, and the working range (0.025, 0.17, and 0.045-0.63 ng/mL, respectively) are better than those for HRP-based CL-ELISA. Furthermore, the analytical parameters of SbP-based CL-ELISA remain unchanged during a long period of time (for at least 30 min). The recovery values from four spiked milk samples with different concentrations of SMP in SbP-based CL-ELISA vary from 70 to 130%.

  3. Egg-Citing! Isolation of Protoporphyrin IX from Brown Eggshells and Its Detection by Optical Spectroscopy and Chemiluminescence

    Science.gov (United States)

    Dean, Michelle L.; Miller, Tyson A.; Bruckner, Christian

    2011-01-01

    A simple and cost-effective laboratory experiment is described that extracts protoporphyrin IX from brown eggshells. The porphyrin is characterized by UV-vis and fluorescence spectroscopy. A chemiluminescence reaction (peroxyoxalate ester fragmentation) is performed that emits light in the UV region. When the porphyrin extract is added as a fluor…

  4. Microbore liquid chromatography of tertiary amine anticholinergic pharmaceuticals with tris(2,2'-bipyridine)ruthenium(III) chemiluminescence detection.

    Science.gov (United States)

    Holeman, J A; Danielson, N D

    1995-06-01

    The post-column chemiluminescent reaction of six anticholinergic alkaloid compounds with tris(2,2'-bipyridine)ruthenium(III) (Ru(bpy)3(3+)) is applied to microbore high-performance liquid chromatography (HPLC). At flow rates less than 200 microL/min, the capillary mixing cell in which Ru(bpy)3(3+) and the analyte are mixed directly allows for good light detection. In contrast, a diminished signal occurs at these low flow rates with conventional post-column mixing in a tee. Optimal chemiluminescent pH conditions for atropine, scopolamine, dicyclomine, cyclopentolate, cyclobenzaprine, and procyclidine are determined at moderately basic conditions (pH 7 to 9). 2-Butanone is found to be compatible with the chemiluminescent reaction, whereas tetrahydrofuran and propionitrile cause an increase in background noise and a chemiluminescent signal loss. As 2-butanone is more nonpolar than acetonitrile, it assists in the elution of these hydrophobic anticholinergic compounds. Five anticholinergic compounds are resolved successfully with a PRP-1 polymeric column and a slightly basic mobile phase, but a C8 silica column is better suited for the more hydrophobic compounds (cyclobenzaprine, procyclidine, and dicyclomine).

  5. A fluorescent and chemiluminescent difunctional mesoporous silica nanoparticle as a label for the ultrasensitive detection of cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Tao Liang [Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi' an 710062 (China); Song Chaojun; Sun Yuanjie [Department of Immunology, The Fourth Military Medical University, Xi' an 710032 (China); Li Xiaohua; Li Yunyun [Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi' an 710062 (China); Jin Boquan [Department of Immunology, The Fourth Military Medical University, Xi' an 710032 (China); Zhang Zhujun, E-mail: zhangzj@snnu.edu.cn [Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi' an 710062 (China); Yang Kun, E-mail: yangkunkun@fmmu.edu.cn [Department of Immunology, The Fourth Military Medical University, Xi' an 710032 (China)

    2013-01-25

    Highlights: Black-Right-Pointing-Pointer Difunctional amino mesoporous silica nanoparticles (FCMSN) were synthesized. Black-Right-Pointing-Pointer The fluorescence and chemiluminescence properties of the FCMSN were studied. Black-Right-Pointing-Pointer The NaIO{sub 4} oxidation method was used for modification of the FCMSN. Black-Right-Pointing-Pointer Liver cancer 7721 cell was detected. Black-Right-Pointing-Pointer The specificity affected by FCMSN's amino groups was studied. - Abstract: A new kind of ultrabright fluorescent and chemiluminescent difunctional mesoporous silica nanoparticle (FCMSN) is reported. A luminescent dye, Rhodamine 6G or tris(2,2 Prime -bipyridyl)dichlororuthenium(II) hexahydrate (Rubpy), is doped inside nanochannels of a silica matrix. The hydrophobic groups in the silica matrix avoid the leakage of dye from open channels. The amines groups on the surface of the FCMSN improve the modification performance of the nanoparticle. Because the nanochannels are isolated by a network skeleton of silica, fluorescence quenching based on the inner filter effect of the fluorescent dyes immobilized in nanochannels is weakened effectively. The Quantum Yield of obtained 90 nm silica particles was about 61%. Compared with the fluorescent core-shell nanoparticle, the chemiluminescence reagents can freely enter the nanoparticles to react with fluorescent dyes to create chemiluminescence. The results show that the FCMSN are both fluorescent labels and chemiluminescent labels. In biological applications, the NaIO{sub 4} oxidation method was proven to be superior to the glutaraldehyde method. The amount of amino could affect the specificity of the FCMSN. The fluorescence microscopy imaging demonstrated that the FCMSN is viable for biological applications.

  6. A novel chemiluminescence assay of mitoxantrone based on diperiodatocuprate(III) oxidation

    Science.gov (United States)

    Yao, Hanchun; Zhang, Min; Zeng, Wenyuan; Zeng, Xiaoying; Zhang, Zhenzhong

    2014-01-01

    A novel and strong chemiluminescence (CL) of luminol with diperiodatocuprate (K5[Cu(HIO6)2]) was observed in alkaline medium. After the addition of mitoxantrone (MTX) into this system, the CL intensity could be greatly inhibited by MTX. Based on the phenomenon, a sensitive CL method was established for analysis of MTX combining with flow injection technology. Under optimum experimental conditions, the CL intensity was linearly related to the logarithm concentration of MTX from 5.0 × 10-9-1.0 × 10-7 g/ml with the detection limit of 1.1 × 10-9 g/ml (S/N = 3). The relative standard deviation was 1.2% for 5.0 × 10-8 g/ml of MTX. The proposed method was successfully applied for determination of MTX in pharmaceutical preparations and biological fluids. The possible CL reaction mechanism was also discussed briefly.

  7. Development of an Electrogenerated Chemiluminescence Biosensor using Carboxylic acid-functionalized MWCNT and Au Nanoparticles

    Directory of Open Access Journals (Sweden)

    2009-03-01

    Full Text Available A COOH-F-MWCNT-Nafion-Ru(bpy32+-Au-ADH electrogenerated chemi-luminescence (ECLelectrode using COOH-functionalized MWCNT (COOH-F-MWCNT and Au nanoparticles synthesized by the radiation method was fabricated for ethanol sensing. A higher sensing efficiency for ethanol for the ECL biosensor prepared by PAAc-g-MWCNT was measured compared to that of the ECL biosensor prepared by PMAc-g-MWCNT, and purified MWCNT. Experimental parameters affecting ethanol detection were also examined in terms of pH and the content of PAAc-g-MWCNT in Nafion. Little interference of other compounds was observed for the assay of ethanol. Results suggest this ECL biosensor could be applied for ethanol detection in real samples.

  8. Development of an Electrogenerated Chemiluminescence Biosensor using Carboxylic acid-functionalized MWCNT and Au Nanoparticles.

    Science.gov (United States)

    Piao, Ming-Hua; Yang, Dae-Soo; Yoon, Kuk-Ro; Lee, Seung-Ho; Choi, Seong-Ho

    2009-01-01

    A COOH-F-MWCNT-Nafion-Ru(bpy)(3) (2+)-Au-ADH electrogenerated chemiluminescence (ECL) electrode using COOH-functionalized MWCNT (COOH-F-MWCNT) and Au nanoparticles synthesized by the radiation method was fabricated for ethanol sensing. A higher sensing efficiency for ethanol for the ECL biosensor prepared by PAAc-g-MWCNT was measured compared to that of the ECL biosensor prepared by PMAc-g-MWCNT, and purified MWCNT. Experimental parameters affecting ethanol detection were also examined in terms of pH and the content of PAAc-g-MWCNT in Nafion. Little interference of other compounds was observed for the assay of ethanol. Results suggest this ECL biosensor could be applied for ethanol detection in real samples.

  9. H2S Analysis in Biological Samples Using Gas Chromatography with Sulfur Chemiluminescence Detection

    Science.gov (United States)

    Vitvitsky, Victor; Banerjee, Ruma

    2015-01-01

    Hydrogen sulfide (H2S) is a metabolite and signaling molecule in biological tissues that regulates many physiological processes. Reliable and sensitive methods for H2S analysis are necessary for a better understanding of H2S biology and for the pharmacological modulation of H2S levels in vivo. In this chapter, we describe the use of gas chromatography coupled to sulfur chemiluminescence detection to measure the rates of H2S production and degradation by tissue homogenates at physiologically relevant concentrations of substrates. This method allows separation of H2S from other sulfur compounds and provides sensitivity of detection to ~15 pg (or 0.5 pmol) of H2S per injected sample. PMID:25725519

  10. A review of electrogenerated chemiluminescent biosensors for assays in biological matrices.

    Science.gov (United States)

    Gross, Erin M; Maddipati, Sai Sujana; Snyder, Sarah M

    2016-10-01

    Electrogenerated chemiluminescence (ECL) is the production of light via electron transfer reactions between electrochemically produced reagents. ECL-based biosensors use specific biological interactions to recognize an analyte and produce a luminescent signal. Biosensors fabricated with novel biorecognition species have increased the number of analytes detected. Some of these analytes include peptides, cells, enzymes and nucleic acids. ECL biosensors are selective, simple, sensitive and have low detection limits. Traditional methods use ruthenium complexes or luminol to generate ECL. Nanomaterials can be incorporated into ECL biosensors to improve efficiency, but also represent a new class of ECL emitters. This article reviews the application of ruthenium complex, luminol and nanomaterial-based ECL biosensors to making measurements in biological matrices over the past 4 years.

  11. Chemiluminescence determination of tetracyclines using Fenton system in the presence europium(III) ions

    Energy Technology Data Exchange (ETDEWEB)

    Kaczmarek, Malgorzata [Department of Rare Earths, Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60 - 780 Poznan (Poland); Lis, Stefan, E-mail: blis@amu.edu.pl [Department of Rare Earths, Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60 - 780 Poznan (Poland)

    2009-04-20

    A new simple chemiluminescent method for the determination of chlortetracycline (Chlor-TC), oxytetracycline (Oxy-TC) and doxycycline (Doxy-TC) is described. This method is based on the europium(III) emission as a result of the energy transfer process from the excited product of the tetracyclines oxidation to the uncomplexed Eu(III). Under the optimum conditions, calibration graphs were obtained for 4 x 10{sup -7} to 2 x 10{sup -5} mol L{sup -1} of Chlor-TC; 2 x 10{sup -7} to 2 x 10{sup -5} mol L{sup -1} of Oxy-TC and 1 x 10{sup -7} to 3 x 10{sup -5} mol L{sup -1} of Doxy-TC. The method was successfully applied to the determination of these drugs in pharmaceutical and veterinary formulation and honey.

  12. Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

    Directory of Open Access Journals (Sweden)

    Jiajia Wang

    2013-01-01

    Full Text Available Based on the inhibitory effect of uric acid (UA on luminol-Co2+ chemiluminescence (CL system, a sensitive method for the determination of UA at nanomolar level by flow injection (FI CL was proposed. The proposed method was successfully applied to real-time monitoring of UA excretion in human 24 h urine with different food intake, showing that meats, vegetables, and porridge intake caused differential UA excretions of 879, 798, and 742 mg, respectively. It was also found that UA concentrations in urine under the three kinds of food intake simultaneously reached maximum at 2 h after meals with the values of 417, 318, and 288 μg mL−1, respectively. The UA concentration in human serum was also determined by this approach, and the possible mechanism of luminol-Co2+-UA CL reaction was discussed in detail.

  13. A New Immunoassay Method by Capillary Electrophoresis with Enhanced Chemiluminescence Detection

    Institute of Scientific and Technical Information of China (English)

    Jiao Ning WANG; Ji Cun REN

    2005-01-01

    This paper described a new immunoassay method by capillary electrophoresis with enhanced chemiluminescence (CL) detection system based on luminol-hydrogen peroxide reaction catalyzed by horseradish peroxides (HRP). Using para-iodophenol as a CL enhancer, the detection limit of about 1×10-12 mol/L for HRP was achieved, which corresponded to 1.32×10-5U/mL. In optimal conditions, the free HRP-labeled CA125 antibody (Ab*) and the bound enzyme-labeled complex (Ab*-Ag) were well separated by capillary electrophoresis within 4 min.The assay was successfully used to determine the contents of CA125 in human sera, which were associated with ovarian cancer, and the recoveries of the standard addition experiments were 96 to109 %.

  14. Cerium(IV)-based chemiluminescence of phentolamine sensitized by rhodamine 6G

    Energy Technology Data Exchange (ETDEWEB)

    Liu Weibing; Huang Yuming

    2004-03-24

    A new chemiluminescence (CL) method is proposed for the determination of phentolamine, which is based on the reaction between studied drug and Cerium(IV) (Ce(IV)) in a nitric acid medium and measurement of the CL intensity produced by rhodamine 6G used as a sensitizer. In the optimum conditions, CL intensities are proportional to concentrations of the studied drug over the range 1x10{sup -9}-1x10{sup -6} g/ml with a detection limit of 4x10{sup -10} g/ml. The relative standard deviation (R.S.D.) is 3.4% for 1x10{sup -7} g/ml phentolamine (n=11). The method has been applied to the determination of studied drug in injections and biological fluids with satisfactory results.

  15. Liquid-phase catalytic reactor combined with measurement of hot electron flux and chemiluminescence

    Science.gov (United States)

    Nedrygailov, Ievgen I.; Lee, Changhwan; Moon, Song Yi; Lee, Hyosun; Park, Jeong Young

    2016-11-01

    Understanding the role of electronically nonadiabatic interactions during chemical reactions on metal surfaces in liquid media is of great importance for a variety of applications including catalysis, electrochemistry, and environmental science. Here, we report the design of an experimental apparatus for detection of the highly excited (hot) electrons created as a result of nonadiabatic energy transfer during the catalytic decomposition of hydrogen peroxide on thin-film metal-semiconductor nanodiodes. The apparatus enables the measurement of hot electron flows and related phenomena (e.g., surface chemiluminescence) as well as the corresponding reaction rates at different temperatures. The products of the chemical reaction can be characterized in the gaseous phase by means of gas chromatography. The combined measurement of hot electron flux, catalytic activity, and light emission can lead to a fundamental understanding of the elementary processes occurring during the heterogeneous catalytic reaction.

  16. Chemiluminescence Sensor for the Determination of Hydroxylamine by Electrostatically Immobilizing Luminol and Periodate

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    A novel chemiluminescence(CL) sensor, which can be used for hydroxylamine determination in combination with flow injection analysis, was developed by electrostatically immobilizing luminol and periodate on anion-exchange resin respectively. Hydroxylamine was sensed by its enhancing effect on the weak CL reaction between luminol and periodate, which were eluted from the ion exchange column. The response of the sensor to hydroxylamine was linear in the concentration range of 8.0×10-8-2.0×10-6 mol/L with a detection limit of 4.0×10-8 mol/L hydroxylamine(3σ). The relative standard deviation(RSD) was 2.0% for 9 repetitive determinations at a hydroxylamine concentration of 5.0×10-7 mol/L. The sensor could be reused for over 400 times with a good reproducibility and was used to determine hydroxylamine in wastewater.

  17. Chemiluminescence and Bioluminescence as an Excitation Source in the Photodynamic Therapy of Cancer: A Critical Review.

    Science.gov (United States)

    Magalhães, Carla M; Esteves da Silva, Joaquim C G; Pinto da Silva, Luís

    2016-08-04

    Photodynamic therapy (PDT) of cancer is known for its limited number of side effects, and requires light, oxygen and photosensitizer. However, PDT is limited by poor penetration of light into deeply localized tissues, and the use of external light sources is required. Thus, researchers have been studying ways to improve the effectiveness of this phototherapy and expand it for the treatment of the deepest cancers, by using chemiluminescent or bioluminescent formulations to excite the photosensitizer by intracellular generation of light. The aim of this Minireview is to give a précis of the most important general chemi-/bioluminescence mechanisms and to analyze several studies that apply them for PDT. These studies have demonstrated the potential of utilizing chemi-/bioluminescence as excitation source in the PDT of cancer, besides combining new approaches to overcome the limitations of this mode of treatment.

  18. Direct chemiluminescence of carbon dots induced by potassium ferricyanide and its analytical application.

    Science.gov (United States)

    Amjadi, Mohammad; Manzoori, Jamshid L; Hallaj, Tooba; Sorouraddin, Mohammad H

    2014-03-25

    The chemiluminescence (CL) of water-soluble fluorescent carbon dots (C-dots) induced by direct chemical oxidation was investigated. C-dots were prepared by solvothermal method and characterized by fluorescence spectra and transmission electron microscopy. It was found that K3Fe(CN)6 could directly oxidize C-dots to produce a relatively intense CL emission. The mechanism of CL generation was investigated based on the fluorescence and CL emission spectra and the effect of radical scavengers on the CL intensity. The inhibitive effect of some metal ions and biologically important molecules on the CL intensity of the system was examined and the potential of the system for the determination of these species at trace levels was studied. In order to evaluate the capability of method to real sample analysis, it was applied to the determination of Cr(VI) and adrenaline in water and injection samples, respectively.

  19. Peroxide induced ultra-weak chemiluminescence and its application in analytical chemistry.

    Science.gov (United States)

    Lin, Zhen; Chen, Hui; Lin, Jin-Ming

    2013-09-21

    Chemiluminescence (CL), as a sensitive, rapid, and facile analytical method, has been widely applied in environmental monitoring, clinical diagnosis and food safety. Recently, the main challenge and research interest in the CL study have been focused on exploring new CL systems and obtaining new insight into the interaction between CL reagents. The peroxide induced ultra-weak CL reactions are some new arising systems that have received great attention and have been successfully applied in many fields. The peroxide includes hydrogen peroxide, peroxynitrite, peroxymonocarbonate, peroxomonosulphate and so on. This review paper covers the mechanism of the peroxide induced ultra-weak CL and the analytical applications of the CL have also been summarized. The future prospects for the peroxide induced ultra-weak CL are discussed.

  20. Chemiluminescence of the peroxomonosulphate-cobalt(II)-aliphatic monocarboxylic acids system.

    Science.gov (United States)

    Wang, Min; Zhao, Lixia; Lin, Jin-Ming

    2007-01-01

    A weak chemiluminescence (CL) emission was observed due to the production of singlet oxygen ((1)O(2)) during the decomposition of peroxomonosulphate (HSO(5)(-)) catalysed by cobalt(II). Low molecular mass aliphatic monocarboxylic acids, such as formic, acetic, propionic, butyric and valeric acids, influenced the CL emission, and the reaction of aliphatic monocarboxylic acids with HSO(5)(-)/Co(2+) solution was further investigated using a flow injection analysis (FIA) CL method. The results indicated that the CL intensities of aliphatic monocarboxylic acids were improved with increase in the carbon chain length in the potassium peroxomonosulphate-cobalt(II) sulphate system. Generation of singlet oxygen was confirmed by the fact that the CL emission of aliphatic monocarboxylic acids with the HSO(5)(-)/Co(2+) solution was quenched by NaN(3), and from the CL spectrum of the reaction system. Additionally, a possible mechanism of aliphatic monocarboxylic acids CL emission enhancement was proposed.

  1. A chemiluminescence sensor array for discriminating natural sugars and artificial sweeteners.

    Science.gov (United States)

    Niu, Weifen; Kong, Hao; Wang, He; Zhang, Yantu; Zhang, Sichun; Zhang, Xinrong

    2012-01-01

    In this paper, we report a chemiluminescence (CL) sensor array based on catalytic nanomaterials for the discrimination of ten sweeteners, including five natural sugars and five artificial sweeteners. The CL response patterns ("fingerprints") can be obtained for a given compound on the nanomaterial array and then identified through linear discriminant analysis (LDA). Moreover, each pure sweetener was quantified based on the emission intensities of selected sensor elements. The linear ranges for these sweeteners lie within 0.05-100 mM, but vary with the type of sweetener. The applicability of this array to real-life samples was demonstrated by applying it to various beverages, and the results showed that the sensor array possesses excellent discrimination power and reversibility.

  2. Chemiluminescence Sensor for the Determination of Hydroxylamine by Electrostatically Immobilizing Luminol and Periodate

    Institute of Scientific and Technical Information of China (English)

    ZHANGSi-Chun; ZHOUGuo-jun; JUHuang-xian

    2003-01-01

    A novel chemiluminescence(CL)sensor,which can be used for hydroxylamine determination in combination with flow injection analysis.was developed by electrostatically immobilizing luminol and periodate on anion-exchange resin respectively.Hydroxylamine was sensed by its enhacing effect on the weak CL reaction between luminol and periodate,which were eluted from the ion exchange column.the response of the sensor to hydroxylamine was lincar in the concentration range of 8.0×10-3~2.0×10-8mol/L with a detection limit of 4.0×10-8mol/L hydroxylamine(3σ).The relative standard deviation(RSD) was 2.0% for 9 repetitive detimes with a good reproducibility and was used to determine hydroxylamine in wastewater.

  3. Applications of capillary electrophoresis with chemiluminescence detection in clinical, environmental and food analysis. A review

    Energy Technology Data Exchange (ETDEWEB)

    Lara, Francisco J.; Airado-Rodríguez, Diego; Moreno-González, David; Huertas-Pérez, José F.; García-Campaña, Ana M., E-mail: amgarcia@ugr.es

    2016-03-24

    This paper reviews the latest developments and analytical applications of chemiluminescence detection coupled to capillary electrophoresis (CE-CL). Different sections considering the most common CL systems have been included, such as the tris(2,2′-bipyridine)ruthenium(II) system, the luminol and acridinium derivative reactions, the peroxyoxalate CL or direct oxidations. Improvements in instrumental designs, new strategies for improving both resolution and sensitivity, and applications in different fields such as clinical, pharmaceutical, environmental and food analysis have been included. This review covers the literature from 2010 to 2015. - Highlights: • An up-to-date critical review about the evolution of CE-CL is presented. • Tris(2,2′-bipyridine)ruthenium(II) and luminol as the most used CL systems. • Instrumental designs and strategies for improving resolution and sensitivity. • Applications in clinical, pharmaceutical, environmental and food analysis.

  4. Design a New Strategy Based on Nanoparticle-Enhanced Chemiluminescence Sensor Array for Biothiols Discrimination

    Science.gov (United States)

    Shahrajabian, Maryam; Hormozi-Nezhad, M. Reza

    2016-08-01

    Array-based sensor is an interesting approach that suggests an alternative to expensive analytical methods. In this work, we introduce a novel, simple, and sensitive nanoparticle-based chemiluminescence (CL) sensor array for discrimination of biothiols (e.g., cysteine, glutathione and glutathione disulfide). The proposed CL sensor array is based on the CL efficiencies of four types of enhanced nanoparticle-based CL systems. The intensity of CL was altered to varying degrees upon interaction with biothiols, producing unique CL response patterns. These distinct CL response patterns were collected as “fingerprints” and were then identified through chemometric methods, including linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA). The developed array was able to successfully differentiate between cysteine, glutathione and glutathione disulfide in a wide concentration range. Moreover, it was applied to distinguish among the above analytes in human plasma.

  5. Chemiluminescence-based multivariate sensing of local equivalence ratios in premixed atmospheric methane-air flames

    Energy Technology Data Exchange (ETDEWEB)

    Tripathi, Markandey M.; Krishnan, Sundar R.; Srinivasan, Kalyan K.; Yueh, Fang-Yu; Singh, Jagdish P.

    2011-09-07

    Chemiluminescence emissions from OH*, CH*, C2, and CO2 formed within the reaction zone of premixed flames depend upon the fuel-air equivalence ratio in the burning mixture. In the present paper, a new partial least square regression (PLS-R) based multivariate sensing methodology is investigated and compared with an OH*/CH* intensity ratio-based calibration model for sensing equivalence ratio in atmospheric methane-air premixed flames. Five replications of spectral data at nine different equivalence ratios ranging from 0.73 to 1.48 were used in the calibration of both models. During model development, the PLS-R model was initially validated with the calibration data set using the leave-one-out cross validation technique. Since the PLS-R model used the entire raw spectral intensities, it did not need the nonlinear background subtraction of CO2 emission that is required for typical OH*/CH* intensity ratio calibrations. An unbiased spectral data set (not used in the PLS-R model development), for 28 different equivalence ratio conditions ranging from 0.71 to 1.67, was used to predict equivalence ratios using the PLS-R and the intensity ratio calibration models. It was found that the equivalence ratios predicted with the PLS-R based multivariate calibration model matched the experimentally measured equivalence ratios within 7%; whereas, the OH*/CH* intensity ratio calibration grossly underpredicted equivalence ratios in comparison to measured equivalence ratios, especially under rich conditions ( > 1.2). The practical implications of the chemiluminescence-based multivariate equivalence ratio sensing methodology are also discussed.

  6. Magnetic-particle-based, ultrasensitive chemiluminescence enzyme immunoassay for free prostate-specific antigen.

    Science.gov (United States)

    Liu, Ruping; Wang, Cheng; Jiang, Quan; Zhang, Wei; Yue, Zhao; Liu, Guohua

    2013-11-01

    We report a magnetic-particle (MMP)-based chemiluminescence enzyme immunoassay (CLEIA) for free prostate-specific antigen (f-PSA) in human serum. In this method, the f-PSA is sandwiched between the anti-PSA antibody coated MMPs and alkaline phosphatase (ALP)-labeled anti-f-PSA antibody. The signal produced by the emitted photons from the chemiluminescent substrate (4-methoxy-4-(3-phosphatephenyl)-spiro-(1,2-dioxetane-3,2'-adamantane)) is directly proportional to the amount of f-PSA in a sample. The present MMP-based assay can detect f-PSA in the range of 0.1-30 ng mL(-1) with the detection limit of 0.1 ng mL(-1). The linear detection range could match the concentration range within the "diagnostic gray zone" of serum f-PSA levels (4-10 ng mL(-1)). The detection limit was sufficient for measuring clinically relevant f-PSA levels (>4 ng mL(-1)). Furthermore, the method was highly selective; it was unaffected by cross-reaction with human glandular kallikrein-2, a kallikrein-like serine protease that is 80% similar to f-PSA. The proposed method was finally applied to determine f-PSA in 40 samples of human sera. Results obtained using the method showed high correlation with those obtained using a commercially available microplate CLEIA kit (correlation coefficient, 0.9821). This strategy shows great potential application in the fabrication of diagnostic kits for determining f-PSA in serum.

  7. Stimulus-response mesoporous silica nanoparticle-based chemiluminescence biosensor for cocaine determination.

    Science.gov (United States)

    Chen, Zhonghui; Tan, Yue; Xu, Kefeng; Zhang, Lan; Qiu, Bin; Guo, Longhua; Lin, Zhenyu; Chen, Guonan

    2016-01-15

    Mesoporous silica nanoparticles (MSN) based controlled release system had been coupled with diverse detection technologies to establish biosensors for different targets. Chemiluminescence (CL) system of luminol/H2O2 owns the characters of simplicity, low cost and high sensitivity, but the targets of which are mostly focused on some oxidants or which can participate in a chemical reaction that yields a product with a role in the CL reaction. In this study, chemiluminescent detection technique had been coupled with mesoporous silica-based controlled released system for the first time to develop a sensitive biosensor for the target which does not cause effect to the CL system itself. Cocaine had been chosen a model target, the MSN support was firstly loaded with glucose, then the positively charged MSN interacted with negatively charged oligonucleotides (the aptamer cocaine) to close the mesopores of MSN. At the present of target, cocaine binds with its aptamer with high affinity; the flexible linear aptamer structured will become stems structured through currently well-defined non-Waston-Crick interactions and causes the releasing of entrapped glucose into the solution. With the assistant of glucose oxidase (GOx), the released glucose can react with the dissolved oxgen to produce gluconic acid and H2O2, the latter can enhance the CL of luminol in the NaOH solution. The enhanced CL intensity has a relationship with the cocaine concentration in the range of 5.0-60μM with the detection limit of 1.43μM. The proposed method had been successfully applied to detect cocaine in serum samples with high selectivity. The same strategy can be applied to develop biosensors for different targets.

  8. Evaluation of nitrogen dioxide chemiluminescence monitors in a polluted urban environment

    Directory of Open Access Journals (Sweden)

    E. J. Dunlea

    2007-01-01

    Full Text Available Data from a recent field campaign in Mexico City are used to evaluate the performance of the EPA Federal Reference Method for monitoring ambient concentrations of NO2. Measurements of NO2 from standard chemiluminescence monitors equipped with molybdenum oxide converters are compared with those from Tunable Infrared Laser Differential Absorption Spectroscopy (TILDAS and Differential Optical Absorption Spectroscopy (DOAS instruments. A significant interference in the chemiluminescence measurement is shown to account for up to 50% of ambient NO2 concentration during afternoon hours. As expected, this interference correlates well with non-NOx reactive nitrogen species (NOz as well as with ambient O3 concentrations, indicating a photochemical source for the interfering species. A combination of ambient gas phase nitric acid and alkyl and multifunctional alkyl nitrates is deduced to be the primary cause of the interference. Observations at four locations at varying proximities to emission sources indicate that the percentage contribution of HNO3 to the interference decreases with time as the air parcel ages. Alkyl and multifunctional alkyl nitrate concentrations are calculated to be reach concentrations as high as several ppb inside the city, on par with the highest values previously observed in other urban locations. Averaged over the MCMA-2003 field campaign, the CL NOx monitor interference resulted in an average measured NO2 concentration up to 22% greater than that from co-located spectroscopic measurements. Thus, this interference has the potential to initiate regulatory action in areas that are close to non-attainment and may mislead atmospheric photochemical models used to assess control strategies for photochemical oxidants.

  9. Rapid determination of Papaver somniferum alkaloids in process streams using monolithic column high-performance liquid chromatography with chemiluminescence detection

    Energy Technology Data Exchange (ETDEWEB)

    Costin, Jason W. [School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3217 (Australia); Lewis, Simon W. [Department of Applied Chemistry, Curtin University of Technology, Perth, WA 6845 (Australia); Purcell, Stuart D. [GlaxoSmithKline, Port Fairy, Victoria 3284 (Australia); Waddell, Lucy R. [GlaxoSmithKline, Port Fairy, Victoria 3284 (Australia); Francis, Paul S. [School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3217 (Australia); Barnett, Neil W. [School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3217 (Australia)]. E-mail: barnie@deakin.edu.au

    2007-07-30

    We have combined high-performance liquid chromatography (HPLC) separations using a monolithic column with acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection in a rapid and highly sensitive method to monitor the process of extracting opiate alkaloids from Papaver somniferum. Due to the high flow rates allowed with the monolithic column and the inherent selectivity of the chemiluminescence reactions, the four predominant alkaloids - morphine, codeine, oripavine and thebaine - were determined in less than 2 min. The results obtained with numerous process samples compared favourable with those of the standard HPLC methodology. Limits of detection were 1 x 10{sup -10} M, 5 x 10{sup -10} M, 5 x 10{sup -10} M and 1 x 10{sup -9} M, for morphine, codeine, oripavine and thebaine, respectively.

  10. Kinetics of nonstationary chemiluminescence during the inhibited oxidation of hydrocarbons and determination of the rate constants for peroxy radical decay

    Energy Technology Data Exchange (ETDEWEB)

    Rusina, I.F.; Emanuel, N.M.; Gagarina, A.B.

    1986-05-01

    This paper presents the results of a theoretical analysis of the kinetics of the nonstationary inhibited chemiluminescence and suggests a method for determining the absolute value of the rate constants for the recombination of peroxy radicals and for their removal by reaction with an inhibitor. From the rate curve for the chemiluminescence in the nonstationary regime following the introduction of an inhibitor it is possible simultaneously and independently to determine the absolute values of the rate constants for recombination of the peroxy radicals and their destruction by the inhibitor. Equations are obtained for calculating the time to establish the quasistationary concentration of peroxy radicals and of radicals formed from the inhibitor, using known values of the constants.

  11. Partial least squares and principal components analysis of wine vintage by high performance liquid chromatography with chemiluminescence detection.

    Science.gov (United States)

    Bellomarino, S A; Parker, R M; Conlan, X A; Barnett, N W; Adams, M J

    2010-09-23

    HPLC with acidic potassium permanganate chemiluminescence detection was employed to analyse 17 Cabernet Sauvignon wines across a range of vintages (1971-2003). Partial least squares regression analysis and principal components analysis was used in order to investigate the relationship between wine composition and vintage. Tartaric acid, vanillic acid, catechin, sinapic acid, ethyl gallate, myricetin, procyanadin B and resveratrol were found to be important components in terms of differences between the vintages.

  12. Effect of size of man-made and natural mineral fibers on chemiluminescent response in human monocyte-derived macrophages.

    OpenAIRE

    2001-01-01

    Fiber size is an important factor in the tumorigenicity of various mineral fibers and asbestos fibers in animal experiments. We examined the time course of the ability to induce lucigenin-dependent chemiluminescence (CL) from human monocyte-derived macrophages exposed to Japan Fibrous Material standard reference samples (glass wool, rock wool, micro glass fiber, two types of refractory ceramic fiber, refractory mullite fiber, potassium titanium whisker, silicon carbide whisker, titanium oxide...

  13. Study on Enhancement Principle and Stabilization for the Luminol-H2O2-HRP Chemiluminescence System.

    Directory of Open Access Journals (Sweden)

    Lihua Yang

    Full Text Available A luminol-H2O2-HRP chemiluminescence system with high relative luminescent intensity (RLU and long stabilization time was investigated. First, the comparative study on the enhancement effect of ten compounds as enhancers to the luminol-H2O2-HRP chemiluminescence system was carried out, and the results showed that 4-(imidazol-1-ylphenol (4-IMP, 4-iodophenol (4-IOP, 4-bromophenol (4-BOP and 4-hydroxy-4'-iodobiphenyl (HIOP had the best performance. Based on the experiment, the four enhancers were dissolved in acetone, acetonitrile, methanol, and dimethylformamide (DMF with various concentrations, the results indicated that 4-IMP, 4-IOP, 4-BOP and HIOP dissolved in DMF with the concentrations of 0.2%, 3.2%, 1.6% and 3.2% could get the highest RLU values. Subsequently, the influences of pH, ionic strength, HRP, 4-IMP, 4-IOP, 4-BOP, HIOP, H2O2 and luminol on the stabilization of the luminol-H2O2-HRP chemiluminescence system were studied, and we found that pH value, ionic strength, 4-IMP, 4-IOP, 4-BOP, HIOP, H2O2 and luminol have little influence on luminescent stabilization, while HRP has a great influence. In different ranges of HRP concentration, different enhancers should be selected. When the concentration is within the range of 0~6 ng/mL, 4-IMP should be selected. When the concentration of HRP ranges from 6 to 25 ng/mL, 4-IOP was the best choice. And when the concentration is within the range of 25~80 ng/mL, HIOP should be selected as the enhancer. Finally, the three well-performing chemiluminescent enhanced solutions (CESs have been further optimized according to the three enhancers (4-IMP, 4-IOP and HIOP in their utilized HRP concentration ranges.

  14. Chemiluminescence of creatinine/H2O2/Co(2+) and its application for selective creatinine detection.

    Science.gov (United States)

    Hanif, Saima; John, Peter; Gao, Wenyue; Saqib, Muhammad; Qi, Liming; Xu, Guobao

    2016-01-15

    Creatinine is an important biomarker in clinical diagnosis and biomonitoring programs as well as urinary metabolomic/metabonomics research. Current methods are either nonselective, time consuming or require heavy and expensive instruments. In this study, chemiluminescence of creatinine with hydrogen peroxide has been reported for the first time, and its chemiluminescence is remarkably enhanced in the presence of cobalt ions. By utilizing these phenomena, we have developed a sensitive and selective chemiluminescence method for creatinine determination by coupling with flow injection analysis. The calibration curve is linear in the range of 1×10(-7)-3×10(-5)mol/L with a limit of detection (S/N=3) of 7.2×10(-8)mol/L, which is adequate for detecting creatinine in the clinically accepted range. The relative standard deviation for seven measurements of 3×10(-5)mol/L creatinine is 1.2%. The chemiluminescence method was then utilized to detect creatinine in human urine samples after simple dilution with water. It takes less than 1min each measurement and the recoveries for spiked urine samples were 100-103%. The interference study demonstrates that some common species in urine, such as amino acids, ascorbic acid and creatine, have negligible effects on creatinine detection. The present method does not use expensive instruments, enzymes and separation technique. This method has the advantages of sensitivity, selectivity, simplicity, rapidity, and low cost. It holds great promise for basic or comprehensive metabolic panel, drug screening, anti-dopping, and urinary metabolomic/metabonomics research.

  15. A Capillary Electrophoresis Detection Scheme for Water-soluble Vitamins Based on Luminol - BrO- Chemiluminescence System

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    A novel chemiluminescence detection scheme has been developed for detecting water-soluble vitamins following capillary electrophoresis (CE) separation. This detection was based on the inhibitory effect of vitamins on the CL reaction between luminol and BrO- in basic aqueous solution. Detection of vitamins was accomplished with a borate-based background electrolyte at pH 9.2. The luminol was used as a component of the separation carrier electrolyte.

  16. Analyzer for measurement of nitrogen oxide concentration by ozone content reduction in gas using solid state chemiluminescent sensor

    Science.gov (United States)

    Chelibanov, V. P.; Ishanin, G. G.; Isaev, L. N.

    2014-05-01

    Role of nitrogen oxide in ambient air is described and analyzed. New method of nitrogen oxide concentration measurement in gas phase is suggested based on ozone concentration measurement with titration by nitrogen oxide. Research of chemiluminescent sensor composition is carried out on experimental stand. The sensor produced on the base of solid state non-activated chemiluminescent composition is applied as ozone sensor. Composition is put on the surface of polymer matrix with developed surface. Sensor compositions includes gallic acid with addition of rodamine-6G. Model of interaction process between sensor composition and ozone has been developed, main products appeared during reaction are identified. The product determining the speed of luminescense appearance is found. This product belongs to quinone class. Then new structure of chemiluminescent composition was suggested, with absence of activation period and with high stability of operation. Experimental model of gas analyzer was constructed and operation algorithm was developed. It was demonstrated that developed NO measuring instrument would be applied for monitoring purposes of ambient air. This work was partially financially supported by Government of Russian Federation, Grant 074-U01

  17. Inhibition of chemiluminescence and chemotactic activity of phagocytes in vitro by the extracts of selected medicinal plants.

    Science.gov (United States)

    Jantan, Ibrahim; Harun, Nurul Hikmah; Septama, Abdi Wira; Murad, Shahnaz; Mesaik, M A

    2011-04-01

    The methanol extracts of 20 selected medicinal plants were investigated for their effects on the respiratory burst of human whole blood, isolated human polymorphonuclear leukocytes (PMNs) and isolated mice macrophages using a luminol/lucigenin-based chemiluminescence assay. We also tested the effect of the extracts on chemotactic migration of PMNs using the Boyden chamber technique. The extracts of Curcuma domestica L., Phyllanthus amarus Schum & Thonn and C. xanthorrhiza Roxb. were the samples producing the strongest oxidative burst of PMNs with luminol-based chemiluminescence, with IC(50) values ranging from 0.5 to 0.7 μg/ml. For macrophage cells, the extracts which showed strong suppressive activity for luminol-based chemiluminescence were C. xanthorrhiza and Garcinia mangostana L. Among the extracts studied, C. mangga Valton & Vazsjip, Piper nigrum L. and Labisia pumila var. alata showed strong inhibitory activity on lucigenin-amplified oxidative burst of PMNs, with IC(50) values ranging from 0.9 to 1.5 μg/ml. The extracts of Zingiber officinale Rosc., Alpinia galangal (L.) Willd and Averrhoa bilimbi Linn showed strong inhibition on the chemotaxic migration of cells, with IC(50) values comparable to that of ibuprofen (1.5 μg/ml). The results suggest that some of these plants were able to modulate the innate immune response of phagocytes at different steps, emphasizing their potential as a source of new immunomodulatory agents.

  18. High-performance liquid chromatographic assay of parabens in wash-off cosmetic products and foods using chemiluminescence detection

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Qunlin [Department of Chemistry, University of Science and Technology of China, Jinzai Road 96, Hefei, Anhui 230026 (China); Lian Mei [Department of Chemistry, University of Science and Technology of China, Jinzai Road 96, Hefei, Anhui 230026 (China); Liu Lijuan [Department of Chemistry, University of Science and Technology of China, Jinzai Road 96, Hefei, Anhui 230026 (China); Cui Hua [Department of Chemistry, University of Science and Technology of China, Jinzai Road 96, Hefei, Anhui 230026 (China)]. E-mail: hcui@ustc.edu.cn

    2005-04-29

    A new method for the simultaneous determination of parabens including methylparaben, ethylparaben, propylparaben, and butylparaben by high-performance liquid chromatography (HPLC) coupled with chemiluminescence detection was developed. The procedure was based on the chemiluminescent enhancement by parabens of the cerium(IV)-rhodamine 6G system in the strong sulfuric acid medium. The good separation of parabens was carried out with an isocratic elution using a mixture of methanol and water (60:40, v/v) within 8.5 min. Under the optimized conditions, a linear working range extends three orders of magnitude with the relative standard deviations of intra- and inter-day precision below 4.5%, and the detection limits were 1.9 x 10{sup -9}, 2.7 x 10{sup -9}, 3.9 x 10{sup -9}, and 5.3 x 10{sup -9} g ml{sup -1} for methylparaben, ethylparaben, propylparaben, and butylparaben, respectively. The chemiluminescence reaction was well compatible with the mobile phase of high-performance liquid chromatography. The proposed method has been successfully applied to the assay of parabens in wash-off cosmetic products and foods with the minimal sample preparation.

  19. Microspot-based ELISA in microfluidics: chemiluminescence and colorimetry detection using integrated thin-film hydrogenated amorphous silicon photodiodes.

    Science.gov (United States)

    Novo, Pedro; Prazeres, Duarte Miguel França; Chu, Virginia; Conde, João Pedro

    2011-12-07

    Microfluidic technology has the potential to decrease the time of analysis and the quantity of sample and reactants required in immunoassays, together with the potential of achieving high sensitivity, multiplexing, and portability. A lab-on-a-chip system was developed and optimized using optical and fluorescence microscopy. Primary antibodies are adsorbed onto the walls of a PDMS-based microchannel via microspotting. This probe antibody is then recognised using secondary FITC or HRP labelled antibodies responsible for providing fluorescence or chemiluminescent and colorimetric signals, respectively. The system incorporated a micron-sized thin-film hydrogenated amorphous silicon photodiode microfabricated on a glass substrate. The primary antibody spots in the PDMS-based microfluidic were precisely aligned with the photodiodes for the direct detection of the antibody-antigen molecular recognition reactions using chemiluminescence and colorimetry. The immunoassay takes ~30 min from assay to the integrated detection. The conditions for probe antibody microspotting and for the flow-through ELISA analysis in the microfluidic format with integrated detection were defined using antibody solutions with concentrations in the nM-μM range. Sequential colorimetric or chemiluminescence detection of specific antibody-antigen molecular recognition was quantitatively detected using the photodiode. Primary antibody surface densities down to 0.182 pmol cm(-2) were detected. Multiplex detection using different microspotted primary antibodies was demonstrated.

  20. Which one of the two common reporter systems is more suitable for chemiluminescent enzyme immunoassay: alkaline phosphatase or horseradish peroxidase?

    Science.gov (United States)

    Yu, Songcheng; Yu, Fei; Liu, Lie; Zhang, Hongquan; Zhang, Zhenzhong; Qu, Lingbo; Wu, Yongjun

    2016-05-01

    Alkaline phosphatase and horseradish peroxidase are the most commonly used reporter systems in chemiluminescent enzyme immunoassay (CLEIA). Which one, therefore, would be better when establishing a CLEIA method for a new target substance? There was no standard answer. In this study, both reporters were compared systematically including luminescence kinetics, conjugation methods, optimal condition and detection performance, using two common drugs, SD-methoxy-pyrimidine and enrofloxacin, as determination objects. The results revealed that there was much difference between the luminescence kinetics of the two systems. However, there was little difference between these systems when detecting the same substance, including in optimal conditions and determination of performance. Both reporters were suitable for establishing chemiluminescent enzyme immunoassays. Therefore, the choice of alkaline phosphatase or horseradish peroxidase as the reporter system in chemiluminescent enzyme immunoassays depends on availability. Conversely, these two report systems could be applied in simultaneous analysis of multicomponents due to their different optical behaviors and similar performances. But attention should be paid to conjugation method and coating buffer, which affected the luminescent intensity of different determination targets.

  1. Laminated paper-based analytical devices (LPAD) with origami-enabled chemiluminescence immunoassay for cotinine detection in mouse serum.

    Science.gov (United States)

    Liu, Wei; Cassano, Christopher L; Xu, Xin; Fan, Z Hugh

    2013-11-01

    Laminated paper-based analytical devices (LPAD) with origami-enabled chemiluminescence immunoassay have been developed for the detection of cotinine, a secondhand smoke (SHS) biomarker. The devices were fabricated by a craft-cutter to define flow channels, followed by lamination. This approach of cutting/lamination to fabricate LPAD is very similar to making an identification card, offering advantages in simplicity and rugged backing when compared to the common method of patterning paper using SU-8 or wax. We also developed a protocol of localized incision and paper-folding to isolate the detection zone from flow channels; the simple origami step eliminated possible reagent diffusion and flow during antibody immobilization steps and numerous washings. By incorporating luminol-based chemiluminescence for detecting horseradish peroxidase-conjugated cotinine, we employed origami-enabled LPAD to detect cotinine in mouse serum using competitive immunoassay. The detection limit was determined to be 5 ng/mL, a clinically relevant concentration. We believe that LPAD with chemiluminescence detection provides a new platform of low cost and sensitive assays for cotinine detection.

  2. Chemiluminescence determination of didanosine%化学发光法测定去羟肌苷

    Institute of Scientific and Technical Information of China (English)

    李建军; 郑伟; 屈凌波; 杨冉

    2009-01-01

    To develop a method for the determination of didanosine by chemiluminescence (CL). Didanosine can greatly inhibit the intensity of the chemiluminescence reaction between KMnO4 and sulphite in a nitric acid medium, and there was a good linear relationship between the inhibition and the concentration of didanosine. Based on this phenomenon, didanosine was determined. The linear range of didanosine was 4.0×10~(-7)~1.0×10~(-4) g/mL and the limit of detection was 9.6×10~(-8) g/mL. The relative standard deviation (re=11) was 2.0% for 8.0×10~(-5) g/mL didanosine. The method could be applied to the determination of didanosine in capsule due to its simplicity, high efficiency and high sensitivity.%建立了快速检测去羟肌苷的化学发光方法. 在HNO3介质中, 去羟肌苷对KMnO_4-Na_2SO_3化学发光体系有较强的抑制作用, 且抑制的程度与去羟肌苷的质量浓度在一定范围内呈良好的线性关系, 据此可以测定去羟肌苷含量. 方法的线性范围为4.0×10~(-7)~1.0×10~(-4) g/mL, 检出限为9.6×10~(-8) g/mL. 对8.0×10~(-5) g/mL去羟肌苷测定的相对标准偏差为2.0% (n=11). 方法适用于胶囊中去羟肌苷的含量测定.

  3. Highly sensitive multianalyte immunochromatographic test strip for rapid chemiluminescent detection of ractopamine and salbutamol

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Hongfei; Han, Jing; Yang, Shijia; Wang, Zhenxing; Wang, Lin; Fu, Zhifeng, E-mail: fuzf@swu.edu.cn

    2014-08-11

    Graphical abstract: A multianalyte immunochromatographic test strip was developed for the rapid detection of two β{sub 2}-agonists. Due to the application of chemiluminescent detection, this quantitative method shows much higher sensitivity. - Highlights: • An immunochromatographic test strip was developed for detection of multiple β{sub 2}-agonists. • The whole assay process can be completed within 20 min. • The proposed method shows much higher sensitivity due to the application of CL detection. • It is a portable analytical tool suitable for field analysis and rapid screening. - Abstract: A novel immunochromatographic assay (ICA) was proposed for rapid and multiple assay of β{sub 2}-agonists, by utilizing ractopamine (RAC) and salbutamol (SAL) as the models. Owing to the introduction of chemiluminescent (CL) approach, the proposed protocol shows much higher sensitivity. In this work, the described ICA was based on a competitive format, and horseradish peroxidase-tagged antibodies were used as highly sensitive CL probes. Quantitative analysis of β{sub 2}-agonists was achieved by recording the CL signals of the probes captured on the two test zones of the nitrocellulose membrane. Under the optimum conditions, RAC and SAL could be detected within the linear ranges of 0.50–40 and 0.10–50 ng mL{sup −1}, with the detection limits of 0.20 and 0.040 ng mL{sup −1} (S/N = 3), respectively. The whole process for multianalyte immunoassay of RAC and SAL can be completed within 20 min. Furthermore, the test strip was validated with spiked swine urine samples and the results showed that this method was reliable in measuring β{sub 2}-agonists in swine urine. This CL-based multianalyte test strip shows a series of advantages such as high sensitivity, ideal selectivity, simple manipulation, high assay efficiency and low cost. Thus, it opens up new pathway for rapid screening and field analysis, and shows a promising prospect in food safety.

  4. Sources of uncertainty in eddy covariance ozone flux measurements made by dry chemiluminescence fast response analysers

    Directory of Open Access Journals (Sweden)

    J. B. A. Muller

    2009-09-01

    Full Text Available Eddy covariance ozone flux measurements are the most direct way to estimate ozone removal near the surface. Over vegetated surfaces, high quality ozone fluxes are required to probe the underlying processes for which it is necessary to separate the flux into the components of stomatal and non-stomatal deposition. Detailed knowledge of the processes that control non-stomatal deposition is limited and more accurate ozone flux measurements are needed to quantify this component of the deposited flux. We present a systematic intercomparison study of eddy covariance ozone flux measurements made using two fast response dry chemiluminescence analysers. Ozone deposition was measured over a well characterised managed grassland near Edinburgh, Scotland, during August 2007. A data quality control procedure specific to these analysers is introduced. Absolute ozone fluxes were calculated based on the relative signals of the dry chemiluminescence analysers using three different calibration methods and the results are compared for both analysers. It is shown that the error in the fitted parameters required for the flux calculations provides a substantial source of uncertainty in the fluxes. The choice of the calculation method itself can also constitute an uncertainty in the flux as the calculated fluxes by the three methods do not agree within error at all times. This finding highlights the need for a consistent and rigorous approach for comparable data-sets, such as e.g. in flux networks. Ozone fluxes calculated by one of the methods were then used to compare the two analysers in more detail. This systematic analyser comparison reveals half-hourly flux values differing by up to a factor of two at times with the difference in mean hourly flux ranging from 0 to 23% with an error in the mean daily flux of ±12%. The comparison of analysers shows that the agreement in fluxes is excellent for some days but that there is an underlying uncertainty as a result of

  5. A novel chemiluminescence reaction system for the determination of norfloxacin with Ag(III) complex

    Science.gov (United States)

    Sun, Hanwen; Chen, Peiyun; Wang, Fei

    2009-10-01

    A novel chemiluminescence (CL) system for the determination of norfloxacin (NFLX) is developed based on the direct CL reaction of [Ag(HIO 6) 2] 5--H 2SO 4-NFLX system. The possible mechanism of CL emission and enhancing effect was discussed by comparing UV, fluorescence and CL spectra. [Ag(HIO 6) 2] 5- in the presence of H 2SO 4 could produce CL emission at 490 nm, this might be caused by the excited state (O 2) 2*. The enhancing effect of NFLX may be produced through an intermolecular energy transfer from part of (O 2) 2* to NFLX molecule and complex of Ag 3+ and NFLX. The CL intensity emission intensity was linear in the range 1.34 × 10 -8 to 5.44 × 10 -6 g mL -1 with correlation coefficient of 0.9982. The detection limit (s/n = 3) was 3.10 × 10 -9 g mL -1. The recovery was in the range of 90.0-104% with the RSD of 1.1-2.8%. The proposed flow injection CL method was applied satisfactorily for the determination of NFLX in capsule, human serum and urine.

  6. Nitrogen doped graphene quantum dots based long-persistent chemiluminescence system for ascorbic acid imaging.

    Science.gov (United States)

    Chen, Hongjun; Wang, Qin; Shen, Qinpeng; Liu, Xin; Li, Wang; Nie, Zhou; Yao, Shouzhuo

    2017-05-15

    High photo-intensity and sluggish flight attenuation are important to highly sensitive chemluminescence imaging. Herein, we present a copper ion catalyzed long-persistent chemiluminescent imaging system of nitrogen-doped graphene quantum dots (NGQDs) for ascorbic acid detection in fruit. NGQDs as luminescent probe are fabricated, emitting out chemluminescence with the direct oxidation by H2O2. In addition, Cu(2+) ion enlarges over two order magnitudes of NGQDs CL intensity (214 times) due to its catalyzed Fenton-like reaction for H2O2 decomposition, and displaying unique specificity against other metal ions. As a result, the twinkling luminescence of NGQDs is boosted and changes to hold persistent with small decay in the presence of copper ion exhibiting potential for CL imaging. As an imaging model, a visual sensor based on Cu(2+)/NGQDs/H2O2 is developed for AA quantitative monitoring with a limit of detection (LOD) of 0.5μM (S/N=3) and applied in real AA detection in fruit. The CL imaging method demonstrated with high stability and proper sensitivity would provide a convenient and visual tool for AA determination, displaying promising candidates for imaging sensing.

  7. Electrogenerated chemiluminescent behavior of MCLA at an indium-tin-oxide electrode

    Energy Technology Data Exchange (ETDEWEB)

    Chen Zhihuang [MOE Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002 (China); Wang Jian [MOE Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002 (China); Lin Zhengyu [MOE Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002 (China); Chen Guonan [MOE Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002 (China)]. E-mail: gnchen@fzu.edu.cn

    2006-08-15

    In this paper, the electrogenerated chemiluminescence (ECL) behavior of 6-(4-methoxyphenyl)-2-methylimidazo[1,2-a]pyrazin-3(7H)-one (MCLA) at an indium-tin-oxide (ITO) electrode in phosphate buffer solution has been investigated when linear sweep voltammetry was applied. The optimum chemical conditions and electrochemical parameters for this ECL system have been investigated in detail. Under the optimum conditions, it was found that the ECL intensity was linear with the concentration of MCLA in the range of 6.0 x 10{sup -7} to 1.0 x 10{sup -5} mol/L, and its detection limit for MCLA was 2.0 x 10{sup -7} mol/L. The possible mechanism for ECL of MCLA at the ITO electrode was also discussion. Furthermore, it was found that some sophora flavescens, such as matrine and oxymatrine, might enhance the ECL intensity of MCLA. Therefore, it is possible to develop a new ECL method for determination of matrine and oxymatrine.

  8. Carbon nanofiber-based luminol-biotin probe for sensitive chemiluminescence detection of protein.

    Science.gov (United States)

    Baj, Stefan; Krawczyk, Tomasz; Pradel, Natalia; Azam, Md Golam; Shibata, Takayuki; Dragusha, Shpend; Skutil, Krzysztof; Pawlyta, Miroslawa; Kai, Masaaki

    2014-01-01

    A carbon nanofiber-based luminol-biotin probe was synthesized for the sensitive chemiluminescence (CL) detection of a target protein by grafting luminol and biotin onto an oxidized carbon nanofiber. This carbon nanofiber was prepared by chemical vapor-deposition with methane in the presence of the Ni-Cu-MgO catalyst, which was followed by oxidization with HNO3-H2SO4 to produce a carboxyl group on the surface of the nanofiber. The material was grafted with luminol and biotin by means of a standard carbodiimide activation of COOH groups to produce corresponding amides. The substance was water-soluble and thus could be utilized as a sensitive CL probe for a protein assay. The probe showed highly specific affinity towards the biotin-labeled antibody via a streptavidin-biotin interaction. The detection limit for this model assay was approximately 0.2 pmol of the biotinized IgG spotted on a polyvinylidene fluoride (PVDF) membrane. Nonspecific binding to other proteins was not observed. Therefore, the synthesized carbon nanofiber-based CL probe may be useful for a sensitive and specific analysis of the target protein.

  9. Improvement on simultaneous determination of chromium species in aqueous solution by ion chromatography and chemiluminescence detection

    DEFF Research Database (Denmark)

    Gammelgaard, Bente; Liao, Y.P.; Jons, O.

    1997-01-01

    A sensitive method for the simultaneous determination of chromium(III) and chromium(VI) was chromatography and chemiluminescence detection. Two Dionex ion-exchange guard columns in series, CG5 and AG7, were used to separate chromium(III) from chromium(VI). Chromium(VI) was reduced by potassium......, the stabilities of reductant and luminol solutions were studied. The linear range of the calibration curve for chromium(III) and chromium(VI) was 1-400 mu g l(-1). The detection limit was 0.12 mu g l(-1) for chromium(III) and 0.09 mu g l(-1) for chromium(VI), respectively. The precision at the 20 mu g l(-1) level...... was 1.4% for chromium(III) and 2.5% for chromium(VI), respectively. The accuracy of the chromium(III) determination was determined by analysis of the NIST standard reference material 1643c, Trace elements in water with the result 19.1 +/- 1.0 mu g Cr(III) l(-1) (certified value 19.0 +/- 0.6 mu g Cr...

  10. Study on immunocapture-chemiluminescence assay of lipase activity in a biological sample.

    Science.gov (United States)

    Ichibangase, Tomoko; Hamabe, Chie; Ohba, Yoshihito; Kishikawa, Naoya; Nakashima, Kenichiro; Kayamori, Yuzo; Kang, Dongchon; Hamasaki, Naotaka; Kuroda, Naotaka

    2006-01-01

    A new approach for the determination of lipase (triacylglycerol lipase, EC.3.1.1.3) activity in a biological sample was investigated by combining an immunocapture technique with a chemiluminescence (CL) assay method in order to eliminate interference with CL detection. The proposed method consists of an immunocapture step to trap lipase and a subsequent step for CL detection of the activity of the captured lipase. The CL detection is based on the luminol-hydrogen peroxide (H(2)O(2))-horseradish peroxidase (HRP) reaction and utilizes a proenhancer substrate [a lauric acid ester of 2-(4-hydroxyphenyl)-4,5-diphenylimidazole (HDI)] which liberates an active enhancer, HDI, by enzymatic hydrolysis. A polyclonal antibody prepared with porcine pancreas lipase was used for the immunocapture. The proposed immunocapture-CL method effectively eliminated the interference with the CL reaction from biological components and enabled the determination of spiked porcine pancreas lipase activity in serum samples in the range 0.41-1.1 U(HDI) (1 U(HDI) corresponds to the amount which liberates 1 pmol HDI/min at 37 degrees C from the substrate). The method was further applied to the assay of the activity for human pancreas lipase in serum and the results showed good correlation (r = 0.871) with those by the conventional colorimetric method.

  11. Flow Injection Analysis with Chemiluminescence detection for Determination of Two Phenothiazines

    Directory of Open Access Journals (Sweden)

    Hijran S. Jabbar

    2015-03-01

    Full Text Available A new flow injection analysis-chemiluminescence (FIA-CL method for determination of two phenothiazine derivatives "chlorpromazine hydrochloride (CPZ and promethazine hydrochloride (PTZ" described. The redox reaction between Fe(III and phenothiazines was taken as a base of this new method. A well-defined volume of CPZ or PTZ and luminol solution is injected simultaneously by means of two injection valves in accordance with the merging zone principle. Calibration graphs were constructed in the range of (0.05 – 1.4μg/ml for CPZ with correlation coefficient of (0.9972 and in the range of (0.5 – 40μg/ml for PTZ with correlation coefficient of (0.9978. The precision and accuracy of the method were checked by calculation of relative standard deviation (RSD and relative error percentage (%E for two different levels of concentrations. The method was relatively free from common excipients and it is applied successfully for the determination of CPZ and PTZ in pharmaceutical formulations. The results obtained were in good agreement with those obtained by a reference method in British Pharmacopoeia.

  12. Simultaneous chemiluminescence determination of thebaine and noscapine using support vector machine regression

    Science.gov (United States)

    Ensafi, Ali A.; Hasanpour, F.; Khayamian, T.; Mokhtari, A.; Taei, M.

    2010-02-01

    In this work, a batch chemiluminescence (CL) method has been proposed for the simultaneous determination of two structurally similar alkaloids, noscapine and thebaine. The method is based on the kinetic distinction of the CL reactions of noscapine and thebaine with Ru(bipy) 32+ and Ce(IV) system in a sulfuric acid medium. The least squared support vector machine (LS-SVM) regression was applied for relating the concentrations of both compounds to their CL profiles. The parameters of the model consisting of σ2 and γ were optimized by constructing LS-SVM models with all possible combinations of these two parameters to select the model with the minimum root mean squared error of cross validation (RMSECV) as the best. The parameters of this model were then selected as optimized values. Under the optimized experimental conditions for both compounds, the detection limits obtained using the LS-SVM regression were 0.08 and 0.1 μmol L -1 for noscapine and thebaine, respectively. The proposed method was utilized for the simultaneous determination of the compounds in pharmaceutical formulations and plasma samples with satisfactory results.

  13. Enhanced chemiluminescence detection of glutathione based on isoluminol-PSM nanoparticles probe.

    Science.gov (United States)

    Ji, Xiaoting; Wang, Wei; Li, Xiaoqian; Chen, Yaoyao; Ding, Caifeng

    2016-04-01

    In this paper, a new chemiluminescence (CL) strategy was constructed for the determination of physiological thiols by using an isoluminol labeled nano-probe. The amino group on the surface of the magnetic beads (MBs) were converted into pyridyl disulfide groups by treatment with N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP), in the meantime, isoluminol and thiolated signal DNA were labeled on the surface of the polystyrene microspheres (PSMs). By treating the SPDP activated MBs with the modified PSMs, isoluminol molecules on the surface of the PSMs, along with the thiolated signal DNA, were attached to the surface of the MBs through disulfide bonds to form a CL probe. In the presence of glutathione (GSH), the disulfide bonds could be cleaved readily. The isoluminol molecules modified on the surface of the PSMs released from the CL probes were detached by magnetic separation and transferred to the dark closet for CL detection of isoluminol-H2O2-HRP system. Using GSH detection as a model, we prove a linear dose response in the range from 5 × 10(-10) to 8 × 10(-8)M. The detection limit of this trial for GSH determined is 5 × 10(-10)M. The proposed design was successfully applied to the extracts of K562 cell for intracellular thiols detection, the average amount of thiols was about 4.114 × 10(-13)M per K562 cell.

  14. Oscillating chemiluminescence in rhodamine B-induced Belousov-Zhabotinsky reaction catalyzed by Ce(Ⅳ)

    Institute of Scientific and Technical Information of China (English)

    DUAN ChunFeng; LIU GuangMing; ZHANG ZhiFeng; CUI Hua

    2009-01-01

    It was found that rhodamine B could induce oscillating chemiluminescence (OCL) from the Ce4+-cata-lyzed Belousov-Zhabotinsky reaction. This new OCL system, i.e., rhodamine B-malonic acid-bromate-Ce(IV)-sulfuric acid, exhibited two clearly distinguished emission peaks in each oscillation period. The initial concentrations of the reactants strongly influenced the oscillation pattern. For the study of the CL mechanism, a platform for a versatile and simultaneous potential and CL measurement was estab-lished to compare the potential oscillation with the CL oscillation behavior of this system. The CL spectra, UV-visible absorption spectra and time-resolved fluorescence spectra of this OCL system were studied. A possible, simplified mechanism for the OCL is proposed. It is suggested that the generation of the two CL peaks is likely due to the oxidation of the intermediate of rhodamine B by Co(IV) and Br2, respectively. This work provided a new method and platform to research the complex chemical oscilla-tions.

  15. Gold Magnetic Nanoparticles-based Chemiluminescent Immunoassay for Detection of Chloramphenicol in Milk

    Directory of Open Access Journals (Sweden)

    Wang Linyu

    2017-01-01

    Full Text Available chemiluminescent immunoassays (CLIA based on gold magnetic nanoparticles (Au-MNPs were developed for rapid analysis of chloramphen icol (CAP in milk sample. Anti-CAP antibodies were immobilized on the surfaces of Au-MNPs, luminol (Method I and 2′,6′-DiMethylcarbonylphenyl-10-sulfopropylacridinium-9-carboxylate 4′-NHS Ester, (NSP-DMAE-NHS, Method II were exploited in competitive CLIA for CAP detection in milk using a homemade luminescent measurement system. The sensitivities and limits of detection (LODs of the two methods were obtained according to the inhibition curves. It indicated that NSP-DMAE-NHS as luminescence reagent (reaction II was more sensitive and effective than luminol (reaction I. The LOD of reaction II reached 0.008 ng/ml while it was 4 ng/ml in reaction I. Moreover, the linear range of the inhibition curve of the former was wider than that of the latter. Such results indicated that the proposed CLIA stratery employing NSP-DMAE-NHS was more sensitive than other immunoassay method for CAP detection.

  16. Chemiluminescence imaging for microRNA detection based on cascade exponential isothermal amplification machinery.

    Science.gov (United States)

    Xu, Yongjie; Li, Dandan; Cheng, Wei; Hu, Rong; Sang, Ye; Yin, Yibing; Ding, Shijia; Ju, Huangxian

    2016-09-14

    A novel G-quadruplex DNAzyme-driven chemiluminescence (CL) imaging method was developed for ultrasensitive and specific detection of miRNA based on the cascade exponential isothermal amplification reaction (EXPAR) machinery. A structurally tailored hairpin probe switch was designed to selectively recognise miRNA and form hybridisation products to trigger polymerase and nicking enzyme machinery, resulting in the generation of product I, which was complementary to a region of the functional linear template. Then, the response of the functional linear template to the generated product I further activated the exponential isothermal amplification machinery, leading to synthesis of numerous horseradish peroxidase mimicking DNAzyme units for CL signal transduction. The amplification paradigm generated a linear response from 10 fM to 100 pM, with a low detection limit of 2.91 fM, and enabled discrimination of target miRNA from a single-base mismatched target. The developed biosensing platform demonstrated the advantages of isothermal, homogeneous, visual detection for miRNA assays, offering a promising tool for clinical diagnosis.

  17. Flow-injection determination of isoniazid using sodium dichloroisocyanurate- and trichloroisocyanuric acid-luminol chemiluminescence systems.

    Science.gov (United States)

    Safavi, A; Karimi, M A; Hormozi Nezhad, M R

    2004-06-01

    A chemiluminescent (CL) method for the determination of isoniazid is described. The method is based on the CL generated during the oxidation of luminol by sodium dichloroisocyanurate (SDCC) and trichloroisocyanuric acid (TCCA) in alkaline medium. It was found that isoniazid greatly enhances this CL intensity when present in the luminol solution. Based on this observation, a new flow-injection CL method for the determination of isoniazid has been proposed in this paper. The detection limits were 2 and 3 ng ml(-1) isoniazid for the SDCC-luminol and TCCA-luminol CL systems, respectively. The relative CL intensity was linear with the isoniazid concentration in the range of 4-100 and 100-200 ng ml(-1) for the SDCC-luminol CL system, and 6-200 and 200-1000 ng ml(-1) for the TCCA-luminol CL system. The results obtained for the assay of pharmaceutical preparations compared well with those obtained by the official methods and demonstrated good accuracy and precision.

  18. Flow injection chemiluminescence determination of hydrazine by oxidation with chlorinated isocyanurates.

    Science.gov (United States)

    Safavi, Afsaneh; Karimi, Mohammad Ali

    2002-10-16

    A rapid and sensitive flow injection chemiluminescence (CL) method is described for the determination of hydrazine based on the CL generated during its reaction with either sodium dichloroisocyanurate (SDCC) or trichloroisocyanuric acid (TCCA) in alkaline medium. The emission intensity is greatly enhanced if dichlorofluorescein (DCF) as sensitizer is present in the reaction medium. The presence of citrate prevents the precipitation of some cations in the reaction medium and also causes an enhancement in emission intensity. The effect of analytical and flow injection variables on these CL systems and determination of hydrazine are discussed. The optimum parameters for the determination of hydrazine were studied and were found to be the following: SDCC and TCCA both 1x10(-3) M; NaOH, 2x10(-1) M; DCF, 5x10(-6) M; citrate, 1x10(-3) M and flow rate, 3.8 ml min(-1). The optimized method yielded 3sigma detection limits of 2x10(-7) and 3x10(-7) M for hydrazine with SDCC and TCCA oxidants, respectively. The method is simple, fast, sensitive, and precise and was applied to the determination of hydrazine in water samples.

  19. Determination of catecholamines by ion chromatography coupled to acidic potassium permanganate chemiluminescence detection

    Institute of Scientific and Technical Information of China (English)

    Hong Wei Wu; Mei Lan Chen; Dan Shou; Yan Zhu

    2012-01-01

    A simple,fast,sensitive,highly selective and eco-friendly analytical method for the determination of catecholamines in human urine by ion chromatography (IC) with chemiluminescence (CL) detection was described in this paper.Using 12 mmoi/L H2SO4 without any organic additive as eluent,three catecholamines including epinephrine (EP),norepinephrine (NE) and dopamine (DA)were well separated on a cation-exchange column.The CL detection was based on the reaction of analytes with acidic potassium permanganate in the presence of formaldehyde as an enhancer.The absence of methanol and acetonitrile in eluent made the proposed method more sensitive and eco-friendly.Under the optimal conditions,the linear range of the proposed method was in the range of 0.02-0.5 μg/mL.The limit of detection (LOD) was in the range of 0.6 and 5.1 μg/L.The relative standard deviations (RSD) for 0.1 μg/mL mixed standard solution were in the range of 0.8-1.9% (n =11).The method has been applied to the determination of catecholamines in human urine successfully.Excellent spiked recoveries were achieved for catecholamines ranged from 91.2% to 112.7%.

  20. A fast and precise chemiluminescence ozone detector for eddy flux and airborne application

    Directory of Open Access Journals (Sweden)

    A. Zahn

    2012-02-01

    Full Text Available A commercially available dry chemiluminescence (CI instrument for fast and precise measurement of ozone (O3 is specified. The sensitivity is ~9000 counts s−1 per ppbv of ozone. Its precision is entirely determined by the number of photons reaching the detector (being a photomultiplier, i.e. is quantum-noise limited. The relative precision (ΔO3/O3 in % thus follows Poisson statistics and scales with the square root of the measurement frequency f and with the inverse O3 mixing ratio: ΔO3/O3f0.5 · O3−0.5. At typical O3 mixing ratios between 10 and 100 ppbv (and 1 bar, the precision is 0.3–1.0% at f = 10 Hz. The maximum measurement frequency is 50 Hz. The mechanical and electronic set-up as well as the instrument performance is described. Recommendations on the adequate inlet tube configuration (inlet tube length, sampling flow and on the way of calibration at stationary ground-based platforms and onboard aircraft are given.

  1. Direct monitoring changes of salbutamol concentration in serum by chemiluminescent imaging.

    Science.gov (United States)

    Zhang, Canli; Zhang, Ruichao; Na, Na; Delanghe, Joris R; Ouyang, Jin

    2011-07-15

    We report in this manuscript, the use of direct ammonium persulfate-enhanced chemiluminescence (CL) imaging, to monitor changes to measure serum salbutamol concentration in subjects of different haptoglobin (Hp) phenotypes at different dosing time. It was noted that CL generated from Hp was decreased due to salbutamol's reducibility, which was used for monitoring salbutamol concentration in serum. The serum from the subjects treated by oral administration of salbutamol, was collected at different dosing time and was separated by polyacrylamide gel electrophoresis (PAGE) prior to the CL detection. According to CL images, samples were separated into three groups based on the Hp phenotypes. The curves of CL signal intensity versus time were obtained for each group, and we demonstrated that there were more significant variables on binding ability between groups. The maximum salbutamol concentration in the serum appeared after 4h, which was in agreement with the literature. In addition, the binding constants of salbutamol to Hp were determined by a fluorescence-based method, whose results were in agreement with the phenomenon of the greater salbutamol metabolism rate for Group Hp 1-1 than Group Hp 2-2. The presented method can monitor changes of salbutamol concentration in serum directly, making the procedures much simple, convenient, rapid and has the property of lower cost. It provided us with excellent reference information for the individual dosage regimen of different Hp groups, which hopefully could become a potential method for further pharmaceutical research.

  2. Chemiluminescence of neutrophiles stimulated by opsonized Zymosan in children with bronchial asthma and pneumonia

    Science.gov (United States)

    Lewandowicz-Uszynska, A.; Jankowski, A.

    2004-08-01

    Oxygen metabolism of neutrophils after stimulation with opsonized zymosan was examined using chemiluminescence test (in the presence of the patient serum or pooled serum). Into the study 37 children aged from 2 to 12 years were enrolled (20 girls and 17 boys). 10 healthy volunteers comprised the control group (group III). Two groups of patients were established: group I -- children with bronchial asthma (without infection), group II -- children with pneumonia. The examination in both groups was performed twice -- in acute phase and in remission period. The group I in acute phase comprised 16 children and in remission phase 9 children, group II - 21 children in acute phase and 9 children in remission phase, respectively. The following parameters of CL were estimated average value of so called spontaneous CL, maximal excitation of neutrophils after stimulation by zymogen (CLmax), time of zymosan opsonization. The following results were obtained: increased spontaneous CL and CLmax (at the presence of both sera) in acute phase of bronchial asthma and pneumonia in comparison to the control group. In the period of remission both these parameters were insignificantly decreased. The longest time of zymosan opsonization in acute period of disease was observed in children with pneumonia (18 min.). This time did not change during remission phase. Only slightly longer time of opsonization was observed in the patients from group I (in exacerbation) (15 min) than in the control group (13,1 min). This time was prolonged in the clinical remission (20 min).

  3. The Chemiluminescence and Structure Properties of Normal/Inverse Diffusion Flames

    Directory of Open Access Journals (Sweden)

    Ting Zhang

    2013-01-01

    Full Text Available The flame emission spectrometry was applied to detect the distribution of excited radicals in two types CH4/O2 coflow jet diffusion flames (normal and inverse diffusion flames. Combining the image analysis along with the spectrometry, the chemiluminescence and structure characteristics of these diffusion flames were investigated. The results show that the inverse diffusion flame (IDF with relatively high inlet oxygen velocity is composed of two regions: a bright base and a tower on top of the base, which is quite different from the normal diffusion flame (NDF. The flame is divided into two regions along the flame axis based on maximum OH* position (Region I: initial reaction zone; Region II: further oxidation zone. The degree of the further oxidization taking place in Region II is obvious in accordance with OH* distribution, which is the main difference in reaction zone between fuel-rich condition and fuel-lean condition for NDFs. For IDFs, the change of OH* distribution with increasing equivalence O/C ratio ([O/C]e in Region II is not conspicuous. More OH* and CH* are generated in IDFs, due to the inner high-speed O2 flow promoting the mixing of fuel and oxygen to a certain extent.

  4. An ultrasensitive electrogenerated chemiluminescence-based immunoassay for specific detection of Zika virus

    Science.gov (United States)

    Acharya, Dhiraj; Bastola, Pradip; Le, Linda; Paul, Amber M.; Fernandez, Estefania; Diamond, Michael S.; Miao, Wujian; Bai, Fengwei

    2016-01-01

    Zika virus (ZIKV) is a globally emerging mosquito-transmitted flavivirus that can cause severe fetal abnormalities, including microcephaly. As such, highly sensitive, specific, and cost-effective diagnostic methods are urgently needed. Here, we report a novel electrogenerated chemiluminescence (ECL)-based immunoassay for ultrasensitive and specific detection of ZIKV in human biological fluids. We loaded polystyrene beads (PSB) with a large number of ECL labels and conjugated them with anti-ZIKV monoclonal antibodies to generate anti-ZIKV-PSBs. These anti-ZIKV-PSBs efficiently captured ZIKV in solution forming ZIKV-anti-ZIKV-PSB complexes, which were subjected to measurement of ECL intensity after further magnetic beads separation. Our results show that the anti-ZIKV-PSBs can capture as little as 1 PFU of ZIKV in 100 μl of saline, human plasma, or human urine. This platform has the potential for development as a cost-effective, rapid and ultrasensitive assay for the detection of ZIKV and possibly other viruses in clinical diagnosis, epidemiologic and vector surveillance, and laboratory research. PMID:27554037

  5. Study on the interaction of catalase with pesticides by flow injection chemiluminescence and molecular docking.

    Science.gov (United States)

    Tan, Xijuan; Wang, Zhuming; Chen, Donghua; Luo, Kai; Xiong, Xunyu; Song, Zhenghua

    2014-08-01

    The interaction mechanisms of catalase (CAT) with pesticides (including organophosphates: disulfoton, isofenphos-methyl, malathion, isocarbophos, dimethoate, dipterex, methamidophos and acephate; carbamates: carbaryl and methomyl; pyrethroids: fenvalerate and deltamethrin) were first investigated by flow injection (FI) chemiluminescence (CL) analysis and molecular docking. By homemade FI-CL model of lg[(I0-I)/I]=lgK+nlg[D], it was found that the binding processes of pesticides to CAT were spontaneous with the apparent binding constants K of 10(3)-10(5) L mol(-1) and the numbers of binding sites about 1.0. The binding abilities of pesticides to CAT followed the order: fenvalerate>deltamethrin>disulfoton>isofenphos-methyl>carbaryl>malathion>isocarbophos>dimethoate>dipterex>acephate>methomyl>methamidophos, which was generally similar to the order of determination sensitivity of pesticides. The thermodynamic parameters revealed that CAT bound with hydrophobic pesticides by hydrophobic interaction force, and with hydrophilic pesticides by hydrogen bond and van der Waals force. The pesticides to CAT molecular docking study showed that pesticides could enter into the cavity locating among the four subdomains of CAT, giving the specific amino acid residues and hydrogen bonds involved in CAT-pesticides interaction. It was also found that the lgK values of pesticides to CAT increased regularly with increasing lgP, Mr, MR and MV, suggesting that the hydrophobicity and steric property of pesticide played essential roles in its binding to CAT.

  6. Effect of tert-butyl hydroperoxide addition on spontaneous chemiluminescence in brain.

    Science.gov (United States)

    Azorin, I; Bella, M C; Iborra, F J; Fornas, E; Renau-Piqueras, J

    1995-12-01

    It is well known that light emission is related to lipid peroxidation in biological material, and that this process occurs spontaneously in the brain. tert-Butyl hydroperoxide (tBHP) is an organic peroxide widely used as initiator of free radical production in several biological systems. However, the prooxidant capacity of this compound remins unclear. To clarify its role in brain spontaneous autooxidation, rat brain homogenates were incubated with and without tBHP. Light emission and lipid peroxidation were measured by luminometry and the TBARs test, respectively. Several inhibitors of free radical-induced lipid peroxidation were also used. These inhibitors included ascorbate, EDTA, and desferrioxamine. Our results indicate that the pattern of light emission spontaneously produced in brain was different from that observed after the addition of tBHP to the homogenates, and that these differences depended on the tBHP concentration. The main difference was that tBHP caused a rapid light emission that reached its maximum more quickly than in the case of spontaneous emission. Addition of ascorbate resulted in an increase in chemiluminescence in presence of tBHP. In contrast, EDTA and desferrioxamine inhibited light emission in homogenates both with and without tBHP. The results of MDA determination were similar to those described, including the effect of inhibitors. A common feature in MDA and luminometric determinations was the dispersion of data. In conclusion, these results suggest that tBHP, under specific conditions, modify the kinetic pattern of brain spontaneous autooxidation.

  7. Chemiluminescence detector based on a single planar transparent digital microfluidic device.

    Science.gov (United States)

    Zeng, Xiangyu; Zhang, Kaidi; Pan, Jian; Chen, Guoping; Liu, Ai-Qun; Fan, Shih-Kang; Zhou, Jia

    2013-07-21

    We report on a compact and portable prototype of chemiluminescence detector based on a single planar single polar transparent electrowetting-on-dielectrics (EWOD) device. The coupling ground model was proposed to build the EWOD device, which could be driven under a single polar voltage. Such a design not only simplified the chip construction and control circuit, but also had the potential for the ball-like droplet to focus the fluorescence and enhance the detection sensitivity. Simulations and experiments both confirmed that the greater the contact angle, the stronger the detected optical signal, and thus the higher the sensitivity. The sensitivity of the prototype detector to H2O2 was 5.45 mV (mmol L(-1))(-1) and the detection limit was 0.01 mmol L(-1) when the contact angle of the EWOD surface was 120°. To further increase the sensitivity and decrease the detection limit, the contact angle of the EWOD device could be increased and the dark current of the photomultiplier decreased. The prototype shows potential applications as highly sensitive, cost effective and portable immuno-detectors, especially as a blood glucose monitor.

  8. Flow injection-chemiluminescence determination of ascorbic acid based on luminol–ferricyanide–gold nanoparticles system

    Energy Technology Data Exchange (ETDEWEB)

    Dong, Yong Ping, E-mail: dongyp@ahut.edu.cn [School of chemistry and chemical engineering, Anhui University of Technology, Maanshan, China, 243002 (China); Gao, Ting Ting; Chu, Xiang Feng; Chen, Jun [School of chemistry and chemical engineering, Anhui University of Technology, Maanshan, China, 243002 (China); Wang, Cheng Ming, E-mail: chmwang@ustc.edu.cn [Hefei National Laboratory for Physical Science at the Microscale, University of Science and Technology of China, Hefei, China, 230026 (China)

    2014-10-15

    A novel flow-injection chemiluminescence (CL) method for the determination of ascorbic acid (AA) is proposed, based upon its enhancing effect on the CL reaction of luminol with ferricyanide catalyzed by gold nanoparticles in alkaline solution. Different sizes gold nanoparticles exhibited different catalyzing effect towards luminol CL and 38 nm gold nanoparticles exhibited the best enhancing effect. Under the optimal experimental conditions, a linear relationship was obtained between the CL intensity and the concentration of ascorbic acid in the range of 1.0×10{sup −10}–1.0×10{sup −6} mol L{sup −1}. The detection limit was 2.0×10{sup –11} mol {sup −1} and the relative standard deviation for 1.0×10{sup −6} mol L{sup −1} ascorbic acid was 0.71% (n=10). This method has been successfully applied in the determination of ascorbic acid in several real samples. - Highlights: • Gold nanoparticles could enhance luminol–K{sub 3}Fe(CN){sub 6} CL signal. • 38 nm gold nanoparticles exhibited the best catalyzing effect. • Ascorbic acid could further enhance luminol–K{sub 3}Fe(CN){sub 6}–gold nanoparticles CL. • Ascorbic acid could be detected sensitively based on its enhancing effect.

  9. Plasma treatment of paper for protein immobilization on paper-based chemiluminescence immunodevice.

    Science.gov (United States)

    Zhao, Mei; Li, Huifang; Liu, Wei; Guo, Yumei; Chu, Weiru

    2016-05-15

    A novel protein immobilization method based on plasma treatment of paper on the low-cost paper-based immunodevice was established in this work. By using a benchtop plasma cleaner, the paper microzone was treated by oxygen plasma treatment for 4 min and then the antibody can be directly immobilized on the paper surface. Aldehyde group was produced after the plasma treatment, which can be verified from the fourier transform infrared spectroscopy (FT-IR) spectra and x-ray photoelectron spectroscopy (XPS) spectra. By linked to aldehyde group, the antibody can be immobilized on the paper surface without any other pretreatment. A paper-based immunodevice was introduced here through this antibody immobilization method. With sandwich chemiluminescence (CL) immunoassay method, the paper-based immunodevice was successfully performed for carcinoembryonic antigen (CEA) detection in human serum with a linear range of 0.1-80.0 ng/mL. The detection limit was 0.03 ng/mL, which was 30 times lower than the clinical CEA level. Comparing to the other protein immobilization methods on paper-based device, this strategy was faster and simpler and had potential applications in point-of-care testing, public health and environmental monitoring.

  10. New bienzymatic strategy for glucose determination by immobilized-gold nanoparticle-enhanced chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Bienzymatic biosensor for the determination of glucose by flow injection chemiluminescence(CL) de-tection was proposed.Hybrids of gold nanoparticles(GNPs) and chitosan were chosen as the immobi-lization matrix of glucose oxidase(GOD) and horseradish peroxidase(HRP) to fabricate the biosensors with silane-pretreated glass microbeads.After the enzyme catalyzing oxidation of glucose in GOD biosensor,the produced H2O2 flowed into HRP biosensor to react with luminol.The doped GNPs in chitosan were found to enhance the classical CL reaction of luminol-H2O2-HRP.The CL enhancement was investigated in detail by CL and UV-visible spectrum.Under the optimized experimental conditions,glucose could be determined in a linear range from 0.01 to 6.0 mmol/L with a detection limit of 5.0 μmol/L at 3σ.The accuracy of the proposed method was examined by detecting the glucose level in four clinical serum samples from hospital.The proposed method provides a new alternative to deter-mine glucose.

  11. New bienzymatic strategy for glucose determination by immobilized-gold nanoparticle-enhanced chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    LIN JieHua; ZHANG Hui; ZHANG ShuSheng

    2009-01-01

    Bienzymatic biosensor for the determination of glucose by flow injection chemiluminescence (CL) de-tection was proposed. Hybrids of gold nanoparticles (GNPs) and chitosan were chosen as the immobi-lization matrix of glucose oxidase (GOD) and horseradish peroxidase (HRP) to fabricate the biosensors with silane-pretreated glass mierobeads. After the enzyme catalyzing oxidation of glucose in GOD biosensor, the produced H2O2 flowed into HRP biosensor to react with luminol. The doped GNPs in chitosan were found to enhance the classical CL reaction of luminol-H2O2-HRP. The CL enhancement was investigated in detail by CL and UV-visible spectrum. Under the optimized experimental conditions, glucose could be determined in a linear range from 0.01 to 6.0 mmol/L with a detection limit of 5.0 μmol/L at 3σ. The accuracy of the proposed method was examined by detecting the glucose level in four clinical serum samples from hospital. The proposed method provides a new alternative to deter-mine glucose.

  12. Chemiluminescence behavior of sodium hydrogen carbonate in the potassium permanganate-hydrogen peroxide reaction

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Chemiluminescence (CL) phenomenon of hydrogen peroxide with potassium permanganate in the presence of sodium hydrogen carbonate was reported.Effects of the surfactant on the CL system were investigated.Nonionic surfactants could effectively increase the CL signal.Radical scavengers and organic reagents such as nitro blue tetrazolium chloride (NBT),cytochrome c,sodium azide,ascorbic acid,thiourea,tert-butanol and dimethyl sulphoxide were used to study the emitting species.CL emission spectrum was recorded and the results showed that the maximal emission wavelengths of NaHCO3-H2O2-KMnO4 system were 440 and 634 nm.The mechanism was discussed based on electron spin resonance (ESR) spectra,fluorescence spectra and UV-vis absorption spectra.The addition of rhodamine B or uranine into this CL system enhanced the CL signal.It was due to part of the energy transfer from singlet oxygen and excited triplet dimers of two CO2 molecules to rhodamine B or uranine.The CL could be induced by excited rhodamine B or uranine.

  13. A chemiluminescence-based continuous flow aqueous ozone analyzer using photoactivated chromotropic acid.

    Science.gov (United States)

    Takayanagi, Toshio; Dasgupta, Purnendu K

    2005-05-15

    Ozone has become the oxidant of choice for water disinfection, especially in large water treatment facilities. This paper describes a fast and sensitive method for the determination of ozone content by reaction with photoactivated chromotropic acid (CA, 4,5-dihydroxynaphthalene-2,7-disulfonic acid), which results in intense chemiluminescence (CL). Freshly ozonated water from a recirculating ozonizer/reservoir is injected into a carrier stream of deionized water in the flow-injection mode. This flow mixes with a stream of photoactivated CA solution in a spiral cell placed directly on top of an inexpensive miniature (8mm diameter active area) photomultiplier tube (PMT). Alkaline CA is photoactivated by passing it through a FEP-Teflon((R)) coil (residence time approximately 50s) wrapped around a 1W UV lamp emitting at 254nm; without photoactivation, the signal is approximately 70-fold lower. The S/N=3 limit of detection for aqueous ozone is 3mugl(-1) and good response slope is obtained up to an ozone concentration of 1.4mgl(-1), the highest that could be made in this study. The response obeyed a quadratic equation with r(2)=0.9984. No interference from permanganate ion is observed. The proposed system was applied to the monitoring of ozonation status of a playa lake water that exhibited significant ozone demand.

  14. Determination of iodide using flow injection with acidic potassium permanganate chemiluminescence detection.

    Science.gov (United States)

    Yaqoob, Mohammad; Atiq-ur-Rehman; Waseem, Amir; Nabi, Abdul

    2006-01-01

    A simple and rapid flow-injection method is described for the determination of iodide, based on potassium permanganate chemiluminescence detection via oxidation of formaldehyde in aqueous hydrochloric acid. The calibration graph was linear over the range 1.0-12 x 10(-6) mol/L (r2 = 0.9955) with relative standard deviations (n = 4) in the range 1.0-3.5%. The detection limit (3sigma) was 1.0 x 10(-7) mol/L, with sample throughput of 120/h. The effect of interfering cations [Ca(II), Mg(II), Ni(II), Fe(II), Fe(III) and Pb(II)] and anions (Cl-, SO4(2-), PO4(3-), NO3-, NO2-, F- and SO3(2-)) were studied. The method was applied to iodized salt samples and the results obtained in the range 0.03 +/- 0.005 - 0.10 +/- 0.006 mg I/g were in reasonable agreement with the amount labelled. The method was statistically compared with the results obtained by titration; no significant disagreement at 95% confidence was observed.

  15. Determination of Prometryn in Vetiver Grass and Water Using Gas Chromatography-Nitrogen Chemiluminescence Detection.

    Science.gov (United States)

    Sun, Shixian; Li, Yongmei; Lv, Ping; Punamiya, Pravin; Sarkar, Dibyendu; Dan, Youming; Ma, Junrong; Zheng, Yi

    2016-02-01

    Nitrogen chemiluminescence detector (NCD) is a nitrogen-specific detector that responds to ammonia, hydrazine, hydrogen cyanide and nitrogen oxide. A method to analyze the herbicide prometryn in plant and water samples was developed using gas chromatograph (GC) coupled with NCD. Extracts from plant (vetiver grass) and water matrices were analyzed for prometryn using an Agilent 7890A GC coupled with an Agilent 255 NCD in a split injection mode with a ratio of 2 : 1. Separation was carried out at 200°C and combustion at 1,018°C with H2 and O2 following optimized method development conditions. The percent recovery of prometryn in the two different matrices tested ranged from 81 to 107%, with relative standard deviations varying from 0.10 to 3.30% for spiked samples. Detection limit of the proposed method was 0.02 µg mL(-1) and the limit of quantification was 0.06 µg mL(-1). The proposed GC-NCD method was successfully applied to determine prometryn extracted from plant and water samples without potential interference of S-triazine, a pesticide from the same group.

  16. Direct determination of acrylamide in food by gas chromatography with nitrogen chemiluminescence detection.

    Science.gov (United States)

    Weijun, Yao

    2015-07-01

    A method of gas chromatography with nitrogen chemiluminescence detection and using standard addition is described for the determination of acrylamide in heat-processed foods. Using a modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) sample preparation method removes the acrylamide precursors completely, and the risk of overestimating acrylamide concentration due to additional analyte formation in the hot gas chromatograph inlet is also avoided. Sample preparation is rapid and inexpensive. A Deans switch device is utilized to heart-cut acrylamide and to prevent interferences from the solvent and matrix from reaching the detector. The pre-column is backflushed at high temperature to maintain a clean baseline and shorten the cycle time compared to baking out the column. Quantitation using standard addition is employed for compensation of potential variability in the acrylamide extraction efficiency in acetonitrile. The limit of detection and the limit of the quantification obtained for this method are 27 and 81 μg/kg, respectively, in food samples (equivalent to 3.5 and 10.6 μg/L in acetonitrile, respectively), and the linear range is 76-9697 μg/kg in food samples (equivalent to 10-1280 μg/L in acetonitrile) with an R(2) value of 0.9999.

  17. Comparing ultraweak bio-chemiluminescence emission in wounded green and etiolated soybean cotyledons

    Science.gov (United States)

    Chen, WenLi; Zhou, Quan; Li, Ling; Xing, Da; Van Wijk, Roeland; Tang, YongHong

    2006-02-01

    Ultraweak bio-chemiluminescence (UBC) from germinating soybean (Glycine max L. Merr) cotyledon under mechanical wounding was observed by a high sensitivity imaging system based on an intensified charge couple device (ICCD) detector or a high sensitive single photon counter (SPC) device. The UBC imaging showed that the intensity of UBC at the injury location on a wounded green cotyledon was greater than on a wounded etiolated cotyledon. Based on results with a SPC UBC intensity of wounded green cotyledon was high at first and then gradually decreased. The emission spectrum of wounded green cotyledon had a greater proportion of red light. The increase in UBC of wounded etiolated soybean cotyledon was less than that of green ones. The emission spectrum of wounded etiolated cotyledon had a greater amount of orange light. The data suggest that most of the UBC in green cotyledon was due to damaged chloroplasts and mitochondria. Our data suggest that oxidation of damage's tissue lead to the production of ROS. Electronic excitation energy was transfered from the excited molecules by ROS to Chl-a in the thylakoid membranes, so the intensity of UBC in the wounded soybean green cotyledon was obviously higher than in the wounded etiolated soybean cotyledon.

  18. Chemiluminescence Imaging of Superoxide Anion Detects Beta-Cell Function and Mass.

    Science.gov (United States)

    Bronsart, Laura L; Stokes, Christian; Contag, Christopher H

    2016-01-01

    Superoxide anion is produced during normal cellular respiration and plays key roles in cellular physiology with its dysregulation being associated with a variety of diseases. Superoxide anion is a short-lived molecule and, therefore, its homeostatic regulation and role in biology and disease requires dynamic quantification with fine temporal resolution. Here we validated coelenterazine as a reporter of intracellular superoxide anion concentration and used it as a dynamic measure both in vitro and in vivo. Chemiluminescence was dependent upon superoxide anion levels, including those produced during cellular respiration, and concentrations varied both kinetically and temporally in response to physiologically relevant fluctuations in glucose levels. In vivo imaging with coelenterazine revealed that beta cells of the pancreas have increased levels of superoxide anion, which acted as a measure of beta-cell function and mass and could predict the susceptibility of mice to diabetes mellitus. Glucose response and regulation are key elements of cellular physiology and organismal biology, and superoxide anion appears to play a fundamental and dynamic role in both of these processes.

  19. Effect of the luminol signal enhancer on the chemiluminescence intensity and kinetics

    Energy Technology Data Exchange (ETDEWEB)

    Liang Yanli [College of Public Health, Zhengzhou University, Zhengzhou 450001 (China); Department of Chemistry, Zhengzhou University, Zhengzhou 450001 (China); Yu Fei; Yu Songcheng [College of Public Health, Zhengzhou University, Zhengzhou 450001 (China); Wu Yongjun, E-mail: wuyongjun@zzu.edu.cn [College of Public Health, Zhengzhou University, Zhengzhou 450001 (China); Department of Chemistry, Zhengzhou University, Zhengzhou 450001 (China); Zhang Hongquan [College of Public Health, Zhengzhou University, Zhengzhou 450001 (China); Qu Lingbo [Department of Chemistry, Zhengzhou University, Zhengzhou 450001 (China)

    2012-04-15

    The novel p-phenol derivatives, 4-(1-imidazolyl)-phenol, 4-hydroxybiphenyl, 4-hydroxy-4 Prime -iodobiphenyl were employed as highly potent signal enhancers of luminol-hydrogen peroxide (H{sub 2}O{sub 2})-horseradish peroxidase (HRP) chemiluminescence (CL) system. The CL reaction conditions were optimized, and the enhancement characteristics of these enhancers were compared with each other. The employment of these molecules greatly affected important assay parameters. Practically, the use of a novel enhancer, even a slightly change of the structure (or concentration) of 4-substituted phenol derivative, could affect assay properties quite dramatically. Furthermore, the use of different enhancers in the luminol-H{sub 2}O{sub 2}-HRP system can affect not only the intensity of the CL signal, which is well known, but also its kinetics. The experiment data indicated that the stronger intensity was combined with a more rapid decrease of the CL signal. - Highlights: Black-Right-Pointing-Pointer 4-IMP, 4-BIP and HIOP take on different signal enhancement and light kinetics. Black-Right-Pointing-Pointer The employment of 4-BIP resulted in a significant improvement of the detection limit. Black-Right-Pointing-Pointer The magnitude of the signal enhancement was about 2.5-fold in the same system.

  20. Selectivity and potential interference from phenolic compounds in chemiluminescence methods for the determination of synephrine.

    Science.gov (United States)

    Francis, Paul S; Brown, Allyson J; Bellomarino, Sara A; Taylor, Amelia M; Slezak, Teo; Barnett, Neil W

    2009-01-01

    Three recently reported chemiluminescence methods (based on reactions with alkaline luminol and hexacyanoferrate(III); acidic cerium(IV) and rhodamine B; and acidic permanganate with polyphosphates) for the determination of synephrine were re-evaluated in terms of their selectivity towards this analyte in comparison to other phenolic compounds. A fourth reagent system, acidic soluble manganese(IV) and formaldehyde, was also examined. Each set of reagents was sensitive towards synephrine (limits of detection were 3 x 10(-9), 5 x 10(-8), 1 x 10(-8) and 1 x 10(-8) mol/L, respectively) but also responded with numerous other phenolic compounds, including some that are present in citrus fruit extracts, dietary supplements and/or biological fluids. It is therefore recommended that the determination of synephrine in these matrices should incorporate physical separation of sample components (e.g. chromatography or electrophoresis). In more general terms, this study illustrates that accurate percentage recoveries for an analyte in spiked samples (without validation against another analytical method) are insufficient to confirm the analytical utility of new flow-injection analysis (FIA) procedures.

  1. High sensitivity chemiluminescence enzyme immunoassay for detecting staphylococcal enterotoxin A in multi-matrices.

    Science.gov (United States)

    Zhang, Chunmei; Liu, Zhijia; Li, Yongming; Li, Qi; Song, Chaojun; Xu, Zhuwei; Zhang, Yun; Zhang, Yusi; Ma, Ying; Sun, Yuanjie; Chen, Lihua; Fang, Liang; Yang, Angang; Yang, Kun; Jin, Boquan

    2013-09-24

    In this study, detection of staphylococcal enterotoxin A (SEA) in multi-matrices using a highly sensitive and specific microplate chemiluminescence enzyme immunoassay (CLEIA) has been established. A pair of monoclonal antibodies (mAbs) was selected from 37 anti-SEA mAbs by pairwise analysis, and the experimental conditions of the CLEIA were optimized. This CLEIA exhibited high performance with a wide dynamic range from 6.4 pg mL(-1) to 1600 pg mL(-1), and the measured low limit of detection (LOD) was 3.2 pg mL(-1). No cross-reactivity was observed when this method was applied to test SEB, SEC1, and SED. It has also been successfully applied for analyzing SEA in a variety of environmental, biological, and clinical matrices, such as sewage, tap water, river water, roast beef, peanut butter, cured ham, 10% nonfat dry milk, milk, orange juice, human urine, and serum. Thus, the highly sensitive and SEA-specific CLEIA should make it attractive for quantifying SEA in public health and diagnosis in near future.

  2. Highly sensitive chemiluminescence technology for protein detection using aptamer-based rolling circle amplification platform

    Institute of Scientific and Technical Information of China (English)

    Zhi-Juan Cao; Qian-Wen Peng; Xue Qiu; Cai-Yun Liu; Jian-Zhong Lu

    2011-01-01

    A robust, selective and highly sensitive chemiluminescent (CL) platform for protein assay was presented in this paper. This novel CL approach utilized rolling circle amplification (RCA) as a signal enhancement technique and the 96-well plate as the immobilization and separation carrier. Typically, the antibody immobilized on the surface of 96-well plate was sandwiched with the protein target and the aptamer-primer sequence. This aptamer-primer sequence was then employed as the primer of RCA. Based on this design, a number of the biotinylated probes and streptavidin-horseradish peroxidase (SA-HRP) were captured on the plate, and the CL signal was amplified. In summary, our results demonstrated a robust biosensor with a detection limit of 10 fM that is easy to be established and utilized, and devoid of light source. Therefore, this new technique .will broaden the perspective for future development of DNA-based biosensors for the detection of other protein biomarkers related to clinical diseases, by taking advantages of high sensitivity and selectivity.

  3. Aptamer-molecularly imprinted sensor base on electrogenerated chemiluminescence energy transfer for detection of lincomycin.

    Science.gov (United States)

    Li, Shuhuai; Liu, Chunhua; Yin, Guihao; Zhang, Qun; Luo, Jinhui; Wu, Nanchun

    2017-05-15

    In this study, a biosensor with a dual recognition system comprising a molecularly imprinted polymer (MIP) and aptamers selective for lincomycin was fabricated. The MIP was synthesized by electropolymerization of carbon dots (C-dots)-tagged DNA aptamers combined with lincomycin and o-aminophenol on the gold-nanoparticle-functionalized graphene oxide (Au-GO)-modified electrode. Electrogenerated chemiluminescence (ECL) resonance energy transfer was observed between Au-GO and C-dots. After the C-dots accepted the energy, they acted as a signal indicator and exhibited enhanced signal intensity in the presence of target lincomycin. When lincomycin was competitively bound to DNA aptamers and MIP, it blocked the transfer of energy, and a decreased ECL signal was observed. Hence, a dual recognition method for the detection of lincomycin is realized. Using this strategy, the sensor exhibited a linear ECL response to lincomycin at concentrations from 5.0 × 10 (-12) mol/L to 1.0 × 10 (-9) mol/L. The detection limit of this assay was found to be 1.6 × 10 (-13) mol/L. This method was utilized to determine lincomycin residuals in meat samples with satisfactory results.

  4. Determination of Melatonin in Rat Pineal Gland and Drug with Flow-Injection Chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    WEI,Shou-Lian; ZHAO,LI-Xia; CHENG,Xiang-Lei; LIN,Jin-Ming

    2007-01-01

    A flow-injection chemiluminescence(CL)method for the determination of melatonin based on the CL reaction of melatonin with hydrogen peroxide and sodium hypochlorite(NaOCl)in a basic alkaline solution was developed.The possible CL mechanism has been discussed,and a proposal for the reaction pathway was given that singlet oxygen was clarified to be produced in this reaction system and was responsible for the CL emission.Under the optimized conditions.the linear concentration range of application was 1.0 X 10-7-2.5×10-4 mol·L-1 with a detection Iimit of 5.0X 10-8 mol·L-1(S/N=3).The relative standard deviation for eight repeated measurements of 1.0×10-6 mol·L-1 melatonin was 2.8%.The interferences of several important biological substances.some indole compound,cations and anions were studied.No interference was found for the anions,glucose,starch,most of cations and low concentration(less than 3.0×10-6 mol·L-1)of some biological substances and indole compound.The method was applied to the determination of melatonin in rat pineal gland and drug with satisfactory results.The sample throughput was 90 injections per hour.

  5. Oxidation of ethyl ether on borate glass: chemiluminescence, mechanism, and development of a sensitive gas sensor.

    Science.gov (United States)

    Hu, Jing; Xu, Kailai; Jia, Yunzhen; Lv, Yi; Li, Yubao; Hou, Xiandeng

    2008-11-01

    A gas sensor was developed by using the chemiluminescence (CL) emission from the oxidation of ethyl ether by oxygen in the air on the surface of borate glass. Theoretical calculation, together with experimental investigation, revealed the main CL reactions: ethyl ether is first oxidized to acetaldehyde and then to acetic acid, during which main luminous intermediates such as CH 3CO (*) are generated and emit light with a peak at 493 nm. At a reaction temperature of 245 degrees C, the overall maximal emission was found at around 460 nm, and the linear range of the CL intensity versus the concentration of ethyl ether was 0.12-51.7 microg mL (-1) ( R = 0.999, n = 7) with a limit of detection (3sigma) of 0.04 microg mL (-1). Interference from foreign substances including alcohol (methanol, ethanol and isopropanol), acetone, ethyl acetate, n-hexane, cyclohexane, dichloromethane, or ether ( n-butyl ether, tetrahydrofuran, propylene oxide, isopropyl ether and methyl tert-butyl ether) was not significant except a minimal signal from n-butyl ether (ethyl ether.

  6. Electrogenerated chemiluminescence biosensor with alcohol dehydrogenase and tris(2,2'-bipyridyl)ruthenium (II) immobilized in sol-gel hybrid material.

    Science.gov (United States)

    Xu, Zhiai; Guo, Zhihui; Dong, Shaojun

    2005-09-15

    An ethanol biosensor based on electrogenerated chemiluminescence detection was developed. Electrogenerated chemiluminescence reagent tris(2,2'-bipyridyl)ruthenium (II) and alcohol dehydrogenase were immobilized in the same sol-gel hybrid film. The copolymer poly(vinyl alcohol) with 4-vinylpyridine and cation exchanger Nafion were incorporated into sol-gel film to provide the microenvironment for retaining the activity of enzyme and immobilize tris(2,2'-bipyridyl)ruthenium (II). The design was simpler than the previous two-layer format. The experimental conditions, such as scan rate, pH and concentration of the cofactor were investigated. The intensity of electrogenerated chemiluminescence increased linearly with ethanol concentration from 2.5x10(-5) to 5.0x10(-2) M and detection limit was 1.0x10(-5) M. The prepared biosensor exhibited high sensitivity, wide linear range and good stability.

  7. Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay

    Directory of Open Access Journals (Sweden)

    Sandra C. P. Cachinho

    2012-01-01

    Full Text Available The oxidative burst of human blood cells in the presence of different metal materials was investigated using chemiluminescence assay. Commercial pure titanium (Ti, titanium alloy (Ti6Al4V, and stainless steel 316L (SS in particulate form with <20 μm in size were used. The effect of particulate materials opsonisation on the upregulation of the respiratory burst production by blood cells was also assessed. The largest chemiluminescence response was achieved after simultaneous injection of the stimulants fMLP+PMA. Moreover, Ti and SS induced a greater inflammatory reaction compared to Ti6Al4V, since the respiratory burst mounted was higher for both materials after opsonisation treatment. These results suggest that in vitro chemiluminescence response and respiratory burst measurements proved to be composition and treatment dependent.

  8. Microplate chemiluminescence enzyme immunoassay for the quantitative evaluation of carbohydrate antigen 72-4 in human serum

    Institute of Scientific and Technical Information of China (English)

    JIN Hui; WANG Xu; XIN TianBing; GAO Peng; LIN JinMing; LIANG ShuXuan

    2008-01-01

    A highly sensitive and specific microplate chemiluminescence enzyme immunoassay (CLEIA) was de-veloped for the quantitative evaluation of carbohydrate antigen 72-4 (CA72-4) in human serum, using luminol-H2O2 catalyzed by horseradish peroxidase (HRP) as the chemiluminescence system. The sim-ple and quick determination was accomplished through a sandwich reaction mode. Several physico-chemical parameters of the immunoreaction, including incubation conditions, antibody coating condi-tions, dilution ratio of anti-CA72-4-HRP conjugate, and chemiluminescence reaction time, were studied and optimized. The proposed method exhibited a linear range of 0-200 U/mL with correlation coeffi-cient and detection limit of 0.9995 and 0.18 U/mL, respectively. The inter-assay and intra-assay coeffi-cients of variation (CV) were both less than 10%. The average recovery of two clinical sera with low and high concentration CA72-4 was 99.3% and 98.7%, respectively. Normal tumor markers, including AFP, CEA, CA2.4-2, CA19-9 and CA15-3, did not cross-react with each other. The method's stability was evaluated by assessing its analytical performance after storing the immunoreagents at 4℃ and 37℃ for 7 days. Little difference was found, indicating satisfactory stability of the method. The present method has been successfully applied to the detection of CA72-4 human serum, and showed a good correlation with the commercially available ELISA kit (r2=0.9383). This method showed great potential in the fabrication of diagnostic kit for CA72-4, and could be well used in diagnosis of cancer in clinical practice.

  9. Evaluation of a new serological test for syphilis based on chemiluminescence assay in a tertiary care hospital

    Directory of Open Access Journals (Sweden)

    Aseem K Tiwari

    2015-01-01

    Full Text Available Context: Syphilis is a transfusion transmissible infections and it is mandatory to do serological test for syphilis (STS on all donor blood samples. STS is usually based on detection of antibodies against the cardiolipin-lecithin antigen or against the Treponema-specific antigen. STS with good sensitivity and specificity helps enhance blood safety and consolidation of STS along with other transfusion transmittable infections such as human immunodeficiency virus, hepatitis-C virus, and hepatitis-B virus helps in reducing the errors and enhances efficiency. Aims: This study was designed to evaluate the performance of newly introduced VITROS ® syphilis Treponema pallidum agglutination (TPA assay based on enhanced chemiluminescence principle for its analytical performance for use as a STS on donor blood samples at a tertiary care health center in National Capital Region, India. Materials and Methods: A total of 108 random blood units collected from the donors (both voluntary and replacement donors and 28 known syphilis sero-reactive samples stored at −20°C, were used to evaluate the performance of VITROS ® syphilis TPA assay based on enhanced chemiluminescence assay on VITROS ® ECiQ immunodiagnostics system along with its analytical performance in terms of its sensitivity, precision, cross-reactivity and interference studies. Results: VITROS ® syphilis TPA showed 100% sensitivity and specificity with precision (20 days study of <10% co-efficient of variation. There was no cross-reactivity with other viral and auto-immune antibodies. No interference was observed from endogenous interfering substances like free hemoglobin or fats. Conclusions: Performance of the VITROS ® syphilis TPA assay meets the requirements for its use as STS in blood bank, thus allowing consolidation with other transfusion transmittable infections screening assay on chemiluminescence platform, which is highly valuable for optimizing workflow and efficiency.

  10. An enhanced chemiluminescence resonance energy transfer aptasensor based on rolling circle amplification and WS2 nanosheet for Staphylococcus aureus detection.

    Science.gov (United States)

    Hao, Liling; Gu, Huajie; Duan, Nuo; Wu, Shijia; Ma, Xiaoyuan; Xia, Yu; Tao, Zui; Wang, Zhouping

    2017-03-22

    A chemiluminescence resonance energy transfer aptasensor was fabricated for the detection of Staphylococcus aureus (S. aureus) with Co(2+) enhanced N-(aminobutyl)-N-(ethylisoluminol) (ABEI) functional flowerlike gold nanoparticles (Co(2+)/ABEI-AuNFs) as donor and WS2 nanosheet as acceptor. In the presence of S. aureus, rolling circle amplification (RCA) can be started. Partially complementary sequence of RCA product functional ABEI-AuNFs (cDNA-ABEI-AuNFs) were then annealed to multiple sites of the RCA product to form duplex complex. This complex is less adsorbed onto the WS2 nanosheet, thus attenuating the quenching of ABEI-AuNFs chemiluminescence by WS2 nanosheet. In the absence of target S. aureus (and hence the absence of RCA and duplex formation), the free cDNA-ABEI-AuNFs is completely adsorbed onto the WS2 nanosheet and chemiluminescence quenching ensues. Under optimal conditions, the logarithmic correlation between the concentration of S. aureus and the CL signal was found to be linear within the range of 50 cfu/mL to 1.5 × 10(5) cfu/mL (R(2) = 0.9913). The limits of detection of the developed method were found to be 15 cfu/mL for S. aureus. The selectivity and the capability of the biosensor in meat samples were also studied. Therefore, this simple and easy operation method can be used to detect S. aureus with high sensitivity and specificity.

  11. Real time imaging of live cell ATP leaking or release events by chemiluminescence microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yun [Iowa State Univ., Ames, IA (United States)

    2008-12-18

    The purpose of this research was to expand the chemiluminescence microscopy applications in live bacterial/mammalian cell imaging and to improve the detection sensitivity for ATP leaking or release events. We first demonstrated that chemiluminescence (CL) imaging can be used to interrogate single bacterial cells. While using a luminometer allows detecting ATP from cell lysate extracted from at least 10 bacterial cells, all previous cell CL detection never reached this sensitivity of single bacteria level. We approached this goal with a different strategy from before: instead of breaking bacterial cell membrane and trying to capture the transiently diluted ATP with the firefly luciferase CL assay, we introduced the firefly luciferase enzyme into bacteria using the modern genetic techniques and placed the CL reaction substrate D-luciferin outside the cells. By damaging the cell membrane with various antibacterial drugs including antibiotics such as Penicillins and bacteriophages, the D-luciferin molecules diffused inside the cell and initiated the reaction that produces CL light. As firefly luciferases are large protein molecules which are retained within the cells before the total rupture and intracellular ATP concentration is high at the millmolar level, the CL reaction of firefly luciferase, ATP and D-luciferin can be kept for a relatively long time within the cells acting as a reaction container to generate enough photons for detection by the extremely sensitive intensified charge coupled device (ICCD) camera. The result was inspiring as various single bacterium lysis and leakage events were monitored with 10-s temporal resolution movies. We also found a new way of enhancing diffusion D-luciferin into cells by dehydrating the bacteria. Then we started with this novel single bacterial CL imaging technique, and applied it for quantifying gene expression levels from individual bacterial cells. Previous published result in single cell gene expression quantification

  12. Electrogenerated chemiluminescence of Si quantum dots in neutral aqueous solution and its biosensing application.

    Science.gov (United States)

    Dong, Yong-Ping; Wang, Jiao; Peng, Ying; Zhu, Jun-Jie

    2017-03-15

    Electrogenerated chemiluminescence (ECL) of semiconductor quantum dots (QDs) has been considered as a powerful technique in the fabrication of biosensor, however, high-toxicity of heavy metal ion containing in QDs severely limits their further applications, and the search for the alternative benign nanomaterials with high ECL efficiency is urgent. Herein, ECL behavior of eco-friendly silicon quantum dots (SiQDs) was reported in neutral aqueous condition. Stable and intense cathodic ECL emission was obtained in phosphate buffer solution (PBS) with K2S2O8 as coreactant. ECL resonance energy transfer (ECL-RET) system was established with SiQDs ECL as energy donor and gold nanoparticles (AuNPs) as energy acceptor, based on which a novel ECL biosensor was fabricated. AuNPs was connected at the terminal of hairpin DNA to form a signal probe. When the probe was modified on SiQDs, ECL-RET occurred due to the short distance between AuNPs and SiQDs, resulting in the apparent decrease of ECL signal. Target DNA can open the loop of hairpin DNA, and move AuNPs away from the electrode surface. As a result, the ECL-RET process was hampered, and the ECL emission resumed. The increased ECL signals varied linearly with the target DNA concentrations in the range of 0.1fM to 1pM with the detection limit of 0.016fM (3σ). The proposed ECL sensor exhibited highly sensitivity and good selectivity in the detection of target DNA.

  13. Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM.

    Science.gov (United States)

    de Ory, Fernando; Minguito, Teodora; Balfagón, Pilar; Sanz, Juan C

    2015-08-01

    In the context of measles elimination, the identification of recent infections is important for clinical laboratories. Serological diagnosis is achieved by detecting specific IgG and IgM. Recently an automated chemiluminescent immunoassay (CLIA) (Liaison, DiaSorin, Italy) has been used to quantify the measles antibody. The aim of this study was to compare this assay with Enzygnost ELISA (Siemens, Germany), with final classification of discrepancies by indirect immunofluorescence (Euroimmun, Germany). For measles IgM, 204 sera were analyzed: 50 IgM-positive, 104 IgM-negative/IgG-positive, and 50 from other viral infections (B19V, rubella, mumps, CMV, and EBV). For the measles IgG assay, 162 samples were tested: 106 were positive and 56 were negative. For measles IgM, the sensitivity and specificity of CLIA against ELISA were 94% (95% CI: 83.2-98.6) and 100% (95% CI: 97.1-100), respectively; the corrected figures after the final classification of discrepancies were 100% (95% CI: 91.0-100) and 99.4% (95% CI: 96.1-100), respectively. In relation to IgG, the sensitivity and specificity of CLIA against ELISA were, respectively, 97.2% (95% CI: 91.7-99.4) and 92.9% (95% CI: 82.5-97.7), and 95.5% (95% CI: 89.5-98.3) and 100% (95% CI: 91.8-100) after the final classification. CLIA showed excellent sensitivity and specificity in detecting measles IgG and IgM antibodies, eliminating the need to aliquot specimens before carrying out the assay.

  14. Application of optimized chemiluminescence assay for determination of the antioxidant capacity of herbal extracts.

    Science.gov (United States)

    Pogačnik, Lea; Ulrih, Nataša Poklar

    2012-01-01

    A chemiluminescence (CL) assay for the determination of antioxidant capacity (AOC) has been optimized and applied to analyses of herbal extracts in the present study. The optimal concentrations of reagents (luminol, H2O2, horseradish peroxidase) have been determined, as well as the optimal reaction conditions (wavelength, pH, temperature, sample volume). All of the measurements were performed at the emission maximum of the oxidized form of luminol (425 nm). The optimal concentrations of the reagents were determined as follows: 1.6 mmol/L luminol, 7.5 mmol/L H2O2 and 0.14 U/mL horseradish peroxidase activity in the reaction mixture. Analyses were carried out in phosphate buffer, pH 7.4, at room temperature. With the optimized CL assay, the AOCs of various water and methanol herbal extracts were determined (dog rose hips, plantain leaves and coltsfoot and thyme flowers) and the results were compared to those obtained by other classical methods for the evaluation of antioxidants. Strong correlations (r > 0.9) with the Folin-Ciocalteau assay and the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH)(●) assay are confirmed, although there is no correlation between AOC and the concentration of ascorbic acid in the samples analysed. This optimized CL assay is simple, rapid and reliable, and it represents a good alternative to classical methods (Folin-Ciocalteau, DPPH(●)) for the determination of AOC of herbal extracts and other food samples.

  15. Paper-based chromatographic chemiluminescence chip for the detection of dichlorvos in vegetables.

    Science.gov (United States)

    Liu, Wei; Kou, Juan; Xing, Huizhong; Li, Baoxin

    2014-02-15

    Paper chromatography was a big breakthrough in the early of 20th century but it is rarely used due to the long separation time and the diffusion on the sample spots. In this work, for the first time, a paper-based chemiluminescence (CL) analytical device combined with paper chromatography was developed for the determination of dichlorvos (DDV) in vegetables without complicated sample pretreatment. The paper chromatography separation procedure can be accomplished in 12 min on a paper support (0.8 × 7.0 cm(2)) by using 5 µL sample spotted on it. After sample developing, the detection area (0.8 × 1.0 cm(2)) was cut and inserted between two layers of water-impermeable single-sided adhesive tapes. The paper-based chip was made by attaching the middle layer of paper onto the bottom layer. Then it was covered by another tape layer, which was patterned by the cutting method to form a square hole (0.8 × 1.0 cm(2)) in it. 10 μL mixed solution of luminol and H2O2 was dropped on the detection area to produce CL. A linear relationship was obtained between the CL intensity and the concentrations of DDV in the range between 10.0 ng mL(-1) and 1.0 μg mL(-1)and the detection limit was 3.6 ng mL(-1). Water-soluble metal ions and vitamins can be developed at different spatial locations relative to DDV, eliminating interference with DDV during detection. The paper-based chromatographic chip can be successfully used for the determination of DDV without complicated sample preparation in vegetables. This study should, therefore, be suitable for rapid and sensitive detection of trace levels of organophosphate pesticides in environmental and food samples.

  16. Pharmacokinetic of pseudoephedrine in rat serum with luminol-pepsin chemiluminescence system by flow injection analysis.

    Science.gov (United States)

    Luo, Kai; Li, Yajuan; Zheng, Xiaohui; Song, Zhenghua

    2015-02-01

    Pepsin (Pep) accelerated the electron transferring rate of excited 3-aminophathlate and enhanced luminol-dissolved oxygen chemiluminescence (CL) intensity, and the flow injection (FI) luminol-Pep CL system was first developed. It was found that the CL intensity of luminol-Pep reaction could be remarkably inhibited by pseudoephedrine (PE); the decrement of CL intensity was linear to the logarithm of PE concentration in the range of 0.1∼100.0 nmol L(-1) with a detection limit of 0.03 nmol mL(-1) (3σ). At a flow rate of 2.0 mL min(-1), the complete process including washing and sampling was performed within 40 s, offering a sample throughput of 90 h(-1). This proposed method was successfully applied to determining PE in rat serum for 18 h after intragastric administration with the elimination ratio of 42.34 % and recoveries from 90.3 to 110.6 %. The pharmacokinetic results showed that PE could be rapidly absorbed into serum with peak concentration (C max) of 1.45 ± 0.18 g L(-1) at the time (T max) of 1.49 ± 0.02 h; the absorption half-life (0.35 ± 0.04 h), elimination half-life (1.86 ± 0.24 h), the area under curve (109.81 ± 6.03 mg L(-1) h(-1)), mean residence time (3.82 ± 0.27 h), and elimination rate constant (2.26 ± 0.23 L g(-1) h(-1)) in rats vivo were derived, respectively. The possible CL mechanism of luminol-Pep-PE reaction was discussed by FI-CL, fluorescence, and molecular docking (MD) methods.

  17. Rapid determination of vitamin B12 concentration with a chemiluminescence lab on a chip.

    Science.gov (United States)

    Lok, Khoi Seng; Abdul Muttalib, Siti Zubaidah binte; Lee, Peter Peng Foo; Kwok, Yien Chian; Nguyen, Nam-Trung

    2012-07-07

    This paper reports a novel method for the rapid determination of vitamin B(12) concentration in a continuous-flow lab-on-a-chip system. This new method is based on luminol-peroxide chemiluminescence (CL) assays for the detection of cobalt(II) ions in vitamin B(12) molecules. The lab-on-a-chip device consisted of two passive micromixers acting as microreactors and a double spiral microchannel network serving as an optical detection region. This system could operate in two modes. In the first mode, samples are acidified and evaluated directly in the microchip. In the second mode, samples are treated externally by acidification prior to detection in the microchip. In the first mode, the linear range obtained was between 1.00 ng ml(-1) to 10 μg ml(-1), R(2) = 0.996, with a relative standard deviation (RSD) of 1.23 to 2.31% (n = 5) and a limit of detection (lod) of 0.368 pg ml(-1). The minimum sample volume required and the analytical time were 30 μl and 3.6 s, respectively. In the second mode, the linear range obtained was between 0.10 ng ml(-1) to 10 μg ml(-1), R(2) = 0.994, with the RSD of 0.90 to 2.32% (n = 6) and a lod of 0.576 pg ml(-1). The minimum sample and the analytical time required were 50 μl and 6 s, respectively. The lab on a chip working in mode II was successfully used for the determination of vitamin B(12) concentrations in nutritional supplemental tablets and hen egg yolks.

  18. Serum dosage of CPK-MB in dogs with ST deviation by chemiluminescence

    Directory of Open Access Journals (Sweden)

    André L.F. Santos

    2014-12-01

    Full Text Available Abstract: Although frequently in humans, hypoxic and ischemic heart diseases are poorly documented in dogs, with only few reports of acute myocardial infarction (AMI in this species. Some electrocardiographic findings might suggest myocardium hypoxia/ischemia, like ST segment elevation or depression, but there are no studies showing whether deviations in ST segment are associated to myocardial injury and serum increase of creatine phosphokinase (CPK-MB. In order to investigate possible myocardial cells injury in poor perfusion conditions, 38 dogs were studied, 20 with normal electrocardiogram and 18 with ST segment elevation or depression, recorded in lead II, at a paper speed of 50 mm/sec and N sensibility (1mV=1cm. Serum measurement of creatine phosphokinase isoenzyme MB (CPK-MB in normal dogs (group 1 determined control values (in ng/mL, which were compared to those obtained from dogs with deviation (group 2, which allowed confirmation or not of myocardial injury. CPK-MB mean values obtained from dogs in groups 1 and 2 were 0.540ng/ml (SD±0.890ng/mL and 0.440ng/mL (SD±1.106, respectively. At a significance level of 5%, the relation of CPK-MB with age, mass and total creatine phosphokinase (CPK-T was not significant in groups 1 and 2. CPK-MB showed no difference, at 5% level, between groups 1 and 2. In conclusion, it is possible to use the human chemiluminescent immunometric assay kit in canine species and that hypoxia/ischemia revealed by ST segment deviation does not mean significant myocardium injury.

  19. Determination of sulpiride in pharmaceutical preparations and biological fluids using a Cr (III) enhanced chemiluminescence method.

    Science.gov (United States)

    Khan, Muhammad Naeem; Jan, Muhammad Rasul; Shah, Jasmin; Lee, Sang Hak; Kim, Young Ho

    2013-01-01

    A highly sensitive and simple method for identifying sulpiride in pharmaceutical formulations and biological fluids is presented. The method is based on increased chemiluminescence (CL) intensity of a luminol-H2O2 system in response to the addition of Cr (III) under alkaline conditions. The CL intensity of the luminol-H2O2-Cr (III) system was greatly enhanced by the addition of sulpiride and the CL intensity was proportional to the concentration of sulpiride in a sample solution. Various parameters affecting the CL intensity were systematically investigated and optimized for determination of the sulpiride in a sample. Under the optimum conditions, the CL intensity was proportional to the concentration of sulpiride in the range of 0.068-4.0 µg/mL, with a good correlation coefficient of 0.997. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 8.50 × 10(-6) µg/mL and 2.83 × 10(-5) µg/mL, respectively. The method presented here produced good reproducibility with a relative standard deviation (RSD) of 2.70% (n = 7). The effects of common excipients and metal ions were studied for their interference effect. The method was validated statistically through recovery studies and successfully applied for the determination of sulpiride in pure form, pharmaceutical preparations and spiked human plasma samples. The percentage recoveries were found to range from 99.10 to 100.05% for pure form, 98.12 to 100.18% for pharmaceutical preparations and 97.9 to 101.4% for spiked human plasma.

  20. Magnetic Fe3O4 nanoparticle catalyzed chemiluminescence for detection of nitric oxide in living cells.

    Science.gov (United States)

    Wang, Huiliang; Li, Mei; Wang, Bing; Wang, Meng; Kurash, Ibrahim; Zhang, Xiangzhi; Feng, Weiyue

    2016-08-01

    Direct and real-time measurement of nitric oxide (NO) in biological media is very difficult due to its transient nature. Fe3O4 nanoparticles (nanoFe3O4) because of their unique catalytic activities have attracted much attention as catalysts in a variety of organic and inorganic reactions. In this work, we have developed a magnetic Fe3O4 nanoparticle-based rapid-capture system for real-time detection of cellular NO. The basic principle is that the nanoFe3O4 can catalyze the decomposition of H2O2 in the system to generate superoxide anion (O2 (·-)) and the O2 (·-) can serve as an effective NO(·) trapping agent yielding peroxynitrite oxide anion, ONOO(-). Then the concentration of NO in cells can be facilely determined via peroxynitrite-induced luminol chemiluminescence. The linear range of the method is from 10(-4) to 10(-8) mol/L, and the detection of limit (3σ, n = 11) is as low as 3.16 × 10(-9) mol/L. By using this method, the NO concentration in 0.1 and 0.5 mg/L LPS-stimulated BV2 cells was measured as 4.9 and 11.3 μM, respectively. Surface measurements by synchrotron X-ray photoelectron spectroscopy (SRXPS) and scanning transmission X-ray microscopy (STXM) demonstrate the catalytic mechanism of the nanoFe3O4-based system is that the significantly excess Fe(II) exists on the surface of nanoFe3O4 and mediates the rapid heterogeneous electron transfer, thus presenting a new Fe2O3 phase on the surface.

  1. Electrogenerated chemiluminescence of luminol using low-cost electrodes; Quimioluminiscencia electrogenerada del luminol usando electrodos de bajo costo

    Energy Technology Data Exchange (ETDEWEB)

    Salgado, Guillermo; Navarrete, Jose [Universidad Finis Terrae, Santiago (Chile). Facultad de Educacion]. E-mail: salgado@interaccess.cl; Bustos, Carlos; Sanchez, Cristian; Ugarte, Ricardo [Universidad Austral de Chile, Valdivia (Chile). Inst. de Quimica

    2006-03-15

    purpose of the study was to observe eletrogenerated chemiluminescence (ECL) of luminol using different materials as electrodes such as wires, nails, coins, razor blades, etc. Based on the experimental observations and the mechanisms proposed in the literature, students should be capable of analyzing their results in order to understand the phenomena studied. Students could then elaborate a mechanism of action consistent with the experimental results which could rationalize the formation of intermediates in the reaction and the dependence of ECL on solution pH in addition to other points of interest. (author)

  2. [Comparative research into sensitivity and specificity of immune-enzyme analysis with chemiluminescence and colorimetric detection for detecting antigens and antibodies to avian influenza viruses and newcastle disease].

    Science.gov (United States)

    Vitkova, O N; Kapustina, T P; Mikhailova, V V; Safonov, G A; Vlasova, N N; Belousova, R V

    2015-01-01

    The goal of this work was to demonstrate the results of the development of the enzyme-linked immunosorbent tests with chemiluminescence detection and colorimetric detection of specific viral antigens and antibodies for identifying the avian influenza and the Newcastle disease viruses: high sensitivity and specificity of the immuno- chemiluminescence assay, which are 10-50 times higher than those of the ELISA colorimetric method. The high effectiveness of the results and the automation of the process of laboratory testing (using a luminometer) allow these methods to be recommended for including in primary screening tests for these infectious diseases.

  3. Emission spectroscopic properties of water soluble porphyrins in hydrogen peroxide chemiluminescence system with d- and f-electron metals

    Science.gov (United States)

    Staninski, Krzysztof; Kaczmarek, Małgorzata; Lis, Stefan; Elbanowski, Marian

    2003-02-01

    Two water-soluble porphyrins: 4,4',4'',4'''-(porphine-5,10,15,20-tetrayl)-tetrakis (benzoic acid) (TCPPH 2) and 4,4',4'',4'''-(porphine-5,10,15,20-tetrayl)-tetrakis (benzenesulfonic acid) (TSPPH 2) have been subjected to spectroscopic study in the presence of d-electron metals: Zn(II) and Cu(II) and f-electron metals: La(III), Eu(III), Gd (III) and Yb(III). Results of the spectrophotometric study have provided evidence proving the complexation of Cu(II) and Zn(II) cations by porphine in water solutions and the complexation of lanthanide ions exclusively by peripheral carboxyl and sulfonic groups. For the first time, chemiluminescence of the systems containing porphyrins has been measured without the use of strongly luminescent reagents such as TCPO or luminol. The emission spectra of the systems porphyrin/metal ion/H 2O 2 have been recorded and the quantum yield of their luminescence has been measured. The absorption spectra of the systems recorded before and after the reaction in the presence of hydrogen peroxide are identical, which means that the porphyrin ring does not undergo destruction. A significant similarity between the fluorescence and chemiluminescence spectra indicates a possibility of excitation of porphyrins and their complexes in the reaction with hydrogen peroxide.

  4. Optimization and development of a high-performance liquid chromatography-based one-site immunometric assay with chemiluminescence detection

    Energy Technology Data Exchange (ETDEWEB)

    Oates, Matthew R.; Clarke, William; Zimlich, Alden; Hage, David S

    2002-10-11

    Various practical and theoretical considerations were examined in the creation and optimization of a high-performance liquid chromatography (HPLC)-based one-site immunometric assay. This method used an HPLC analyte analog column and post-column chemiluminescence detection. The specific analyte chosen as the model for this study was L-thyroxine (also known as T{sub 4}). In this technique, a sample containing thyroxine was first combined with an excess of anti-T{sub 4} antibody Fab fragments that had earlier been conjugated with chemiluminescent acridinium ester labels. After incubation, the mixture was injected onto a column that contained immobilized T{sub 4}. The amount of thyroxine in the original sample was then determined by measuring the labeled Fab fragments that appeared in the non-retained fraction, or the decrease in excess Fab fragments that were bound to and later eluted from the column. Items considered in creating this assay included the preparation of acridinium ester-labeled Fab fragments, the detection of these fragments with a post-column reactor, and the creation of a suitable immobilized analog column for capturing excess labeled Fab fragments. The final method could measure T{sub 4} in standards at clinically-relevant concentrations and provided a response within 1.5 min of sample injection, following a 20-45 min incubation with the labeled Fab fragments. Possible applications of this method include its use in clinical chemistry and the screening of proteomic or combinatorial libraries.

  5. Flow-injection chemiluminescence determination of phentolamine based on its enhancing effect on the luminol-potassium ferricyanide system.

    Science.gov (United States)

    Huang, Yuming; Liu, Weibing

    2005-07-01

    It was found that the light emission produced by the oxidation of luminol by potassium ferricyanide in the basic medium was enhanced by phentolamine, a drug recently used to treatment of male and female sexual dysfunction. The optimum conditions for this chemiluminescent reaction were studied in detail by a flow-injection system. A new, simple and rapid method has been developed under the optimum conditions for determination of phentolamine. This method has the advantages of high sensitivity, good reproducibility and low detection limit. On the basis of investigation of chemiluminescent, fluorescent and UV spectra of phentolamine in basic solution containing potassium ferricyanide and luminol, a possible mechanism of this reaction was proposed. In the optimum conditions, CL intensities are proportional to concentrations of the phentolamine in the 0.01-1 microg/mL range. The limit of detection is 3.0 ng/mL for phentolamine. The method has been applied to the determination of phentolamine in the commercial preparations, synthetic samples and biological fluids with satisfactory results.

  6. A sensitive chemiluminescent enzyme immunoassay for the bioanalysis of carboxyl-terminal B-chain analogues of human insulin.

    Science.gov (United States)

    Cao, Y; Smith, W C; Bowsher, R R

    2001-08-01

    Quantification of analogues of human insulin in biological matrices is complicated by differences in their immunoreactivity and the presence of both the analogue and endogenous concentrations of insulin in test samples. To facilitate pharmacokinetic comparisons of carboxyl-terminal B-chain analogues of human insulin, we undertook development of a sensitive ELISA. The ELISA detection method was optimized systematically to permit routine analysis of 10-microl serum samples. Accordingly, a noncompetitive 'sandwich' chemiluminescent ELISA was validated for the quantification of carboxyl-terminal B-chain insulin analogues in human serum over a concentration range from 5 to 3125 pM. The mean bias (RE%) within the validated range varied from -10.3 to 4.3%, with an intermediate precision (inter-assay CV%) from 4.2 to 11.5%. The two-sided 90% expectation tolerance interval for total measurement error was within +/-25% of the nominal concentration for all levels of validation samples. Insulin lispro, human insulin, proinsulin, despentapeptide insulin (DPI) and porcine insulin displayed comparable crossreactivity in the ELISA. Potential utility of the new assay for insulin bioanalysis in nonhuman species was investigated by assessing the pharmacokinetic profile of DPI in rats following administration of a single subcutaneous dose. The sensitive chemiluminescent detection method is simple to perform and should be readily adaptable for ELISAs of other therapeutic proteins.

  7. Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination

    Directory of Open Access Journals (Sweden)

    Muhammad Asgher

    2014-01-01

    Full Text Available Rose Bengal photosensitized flow injection chemiluminescence method is reported using luminol-Cu(II for the determination of vitamins A and C in pharmaceutical formulations. The reaction is based on the enhancement effect of analyte in the production of anion radicals of Rose Bengal (RB•− which rapidly interact with dissolved oxygen and generate superoxide anions radicals (O2•− and hydrogen peroxide (H2O2. Highly reactive hydroxyl radicals (•OH were produced via dismutation of H2O2 by catalyst (Cu2+. The generated superoxide anions radicals and hydroxyl radicals thus oxidize luminol in alkaline medium to generate strong chemiluminescence. The limit of detection (3s of the blank, n=6 of vitamins A and C and RB was found to be 0.008, 0.005, and 0.05 μg mL−1, respectively. The sample throughput of 70 h−1 for vitamins A and C and 30 h−1 for RB was found. Calibration curve was linear in the range of 0.05–15, 0.01–20, and 0.1–50 μg mL−1 for vitamins A and C and RB, respectively, with relative standard deviations (RSDs; n=3 in the range 1.6–3.6%. The method was successfully applied to pharmaceutical formulations and the results obtained were in good agreement with the labeled values.

  8. Parabens determination in cosmetic and personal care products exploiting a multi-syringe chromatographic (MSC) system and chemiluminescent detection.

    Science.gov (United States)

    Rodas, Melisa; Portugal, Lindomar A; Avivar, Jessica; Estela, José Manuel; Cerdà, Víctor

    2015-10-01

    Parabens are widely used in dairy products, such as in cosmetics and personal care products. Thus, in this work a multi-syringe chromatographic (MSC) system is proposed for the first time for the determination of four parabens: methylparaben (MP), ethylparaben (EP), propylparaben (PP) and butylparaben (BP) in cosmetics and personal care products, as a simpler, practical, and low cost alternative to HPLC methods. Separation was achieved using a 5mm-long precolumn of reversed phase C18 and multi-isocratic separation, i.e. using two consecutive mobile phases, 12:88 acetonitrile:water and 28:72 acetonitrile:water. The use of a multi-syringe buret allowed the easy implementation of chemiluminescent (CL) detection after separation. The chemiluminescent detection is based on the reduction of Ce(IV) by p-hydroxybenzoic acid, product of the acid hydrolysis of parabens, to excite rhodamine 6G (Rho 6G) and measure the resulting light emission. Multivariate designs combined with the concepts of multiple response treatments and desirability functions have been employed to simultaneously optimize and evaluate the responses. The optimized method has proved to be sensitive and precise, obtaining limits of detection between 20 and 40 µg L(-1) and RSD <4.9% in all cases. The method was satisfactorily applied to cosmetics and personal care products, obtaining no significant differences at a confidence level of 95% comparing with the HPLC reference method.

  9. Chemiluminescent method for detection of eutrophication sources by estimation of organic amino nitrogen and ammonium in water.

    Science.gov (United States)

    Meseguer-Lloret, S; Molins-Legua, C; Verdú-Andrés, J; Campíns-Falcó, P

    2006-11-01

    An automatic method has been developed for the estimation of organic amino nitrogen (CH2-NH) and ammonium in water samples. We propose a continuous flow system in which nitrogen compounds react with hypochlorite reagent to produce chloramines. Subsequently, the mixture is mixed with luminol, generating a chemiluminescence signal. The signal emission at 425 nm, registered as a function of time, decreases as nitrogen concentration increases, due to the decrease on hypochlorite concentration. A large number of nitrogen compounds have been assayed and their sensitivities compared, in milligrams per liter nitrogen. The ammonium calibration graph, expressed as N, can be used for most of the assayed compounds. The linear interval was 0.24-4 mg L(-1) N, with the detection limit 0.07 mg L(-1) N. The chemiluminescence method was applied to the analysis of several kinds of real water samples, natural, lake, irrigation ditch, fountain, residual, and seawater in order to detect possible sources of eutrophication. The accuracy (% relative error) and precision were satisfactory, with mean values of 5 +/- 4 and 3 +/- 2, respectively. This procedure has been used to estimate nitrogen content in samples before and after Kjeldahl treatment. In the same samples, the N found for the untreated samples provided a good estimation of the N Kjeldahl. Sixty samples per hour can be analyzed, and the procedure can also be used for in situ monitoring.

  10. Study on superoxide and hydroxyl radicals generated in indirect electrochemical oxidation by chemiluminescence and UV-visible spectra

    Institute of Scientific and Technical Information of China (English)

    Zhang Botao; Zhao Lixia; Lin Jinming

    2008-01-01

    The generation and transformation of radicals on the cathode of indirect electrochemical oxidation were studied by chemiluminescence (CL) and UV-visible spectra in the reactor with a salt bridge that connected the separated chambers. The CL intensity of 4 × 10-9 mol/L luminol on the cathode with bubbling oxygen was about seven times that of the intensity without it, which was because of the generation of reactive oxygen species (ROS). The existence of ROS, especially the generation of the superoxide radical, could be affirmed by the fact that the CL intensity of 4 × 10--9 mol/L 2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one with bubbling oxygen was about four times that of the intensity without it. However, there was no chemiluminescence on the anode under the same condition. The change in the UV-visible spectra of nitro blue tetrazolium and N,N-dimethyl-4-nitrosoaniline at the cathode chamber affirmed the transformation from oxygen to superoxide and hydroxyl radicals. The mechanism of the superoxide and hydroxyl radical generation and transformation on the cathode was discussed with the help of the experimental results and relative references.

  11. Use of biotin-labeled nucleic acids for protein purification and agarose-based chemiluminescent electromobility shift assays.

    Science.gov (United States)

    Rodgers, J T; Patel, P; Hennes, J L; Bolognia, S L; Mascotti, D P

    2000-01-15

    We have employed biotin-labeled RNA to serve two functions. In one, the biotin tethers the RNA to streptavidin-agarose beads, creating an affinity resin for protein purification. In the other, the biotin functions as a label for use in a modified chemiluminescent electromobility shift assay (EMSA), a technique used to detect the formation of protein-RNA complexes. The EMSA that we describe avoids the use not only of radioactivity but also of neurotoxic acrylamide by using agarose as the gel matrix in which the free nucleic acid is separated from protein-nucleic acid complexes. After separation of free from complexed RNA in agarose, the RNA is electroblotted to positively charged nylon. The biotin-labeled RNA is readily bound by a streptavidin-alkaline phosphatase conjugate, allowing for very sensitive chemiluminescent detection ( approximately 0.1-1.0 fmol limit). Using our system, we were able to purify both known iron-responsive proteins (IRPs) from rat liver and assess their binding affinity to RNA containing the iron-responsive element (IRE) using the same batch of biotinylated RNA. We show data indicating that agarose is especially useful for cases when large complexes are formed, although smaller complexes are even better resolved.

  12. Chemiluminescence enzyme immunoassay based on magnetic nanoparticles for detection of hepatoceUular carcinoma marker glypican-3

    Institute of Scientific and Technical Information of China (English)

    Qian-Yun Zhang; Hui Chen; Zhen Lin; Jin-Ming Lin

    2011-01-01

    Glypican-3 (GPC3) is reported as a great promising tumor marker for hepatocellular carcinoma (HCC) diagnosis. Highly sensitive and accurate analysis of serum GPC3 (sGPC3), in combination with or instead of traditional HCC marker alpha-fetoprotein (AFP), is essential for early diagnosis of I-ICC. Biomaterial-functionalized magnetic particles have been utilized as solid supports with good biological compatibility for sensitive immunoassay. Here, the magnetic nanoparticles (MnPs) and magnetic microparticles (MmPs) with carboxyl groups were further modified with streptavidin, and applied for the development of chemiluminescence enzyme immunoassay (CLEIA). After comparing between MnPs- and MmPs-based CLEIA, MnPs-based CLEIA was proved to be a better method with less assay time, greater sensitivity, better linearity and longer chemiluminescence platform. MnPs-based CLEIA was applied for detection of sGPC3 in normal liver, hepatocirrhosis, secondary liver cancer and HCC serum samples. The results indicated that sGPC3 was effective in diagnosis of HCC with high performance.

  13. Study on the luminescence behavior of lanthanide ions with luminol by flow injection chemiluminescence analysis

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Xiaoqing [College of Chemistry and Material Science, Northwest University, 710069 Xi' an (China); Wang, Zhuming [College of Earth Sciences and Land Resources, Chang' an University, 710054 Xi' an (China); Li, Yajuan [College of Chemistry and Material Science, Northwest University, 710069 Xi' an (China); Zhang, Yun [Department of pharmacy, Xi' an Medical University, 710021 Xi' an (China); Guo, Jie; Zhao, Jingchan [College of Chemistry and Material Science, Northwest University, 710069 Xi' an (China); Song, Zhenghua, E-mail: songzhenghua@hotmail.com [College of Chemistry and Material Science, Northwest University, 710069 Xi' an (China)

    2015-06-15

    This work first described the linear quenching of Ln{sup III} (including La{sup III}, Ce{sup III}, Pr{sup III}, Nd{sup III}, Sm{sup III}, Eu{sup III}, Gd{sup III}, Tb{sup III}, Dy{sup III}, Ho{sup III}, Er{sup III}, Tm{sup III}, Yb{sup III} and Lu{sup III}) on luminol–dissolved oxygen chemiluminescence system and possible quenching mechanism by flow injection analysis. The general equation of the decrement of CL intensity with Ln{sup III} concentration, ΔI=AlnC{sub Ln}+B, was given and the interaction model of Ln{sup III} with luminol, lg[(I{sub 0}−I)/I]=rlg[Ln]+lgK, was established. The sensitive factor A was found to be linear with the physical parameters (Z, γ{sub ±}, E{sup o} and ΔH{sub hyd}). By plotting A against Z, it could be seen light lanthanides (LL) and heavy lanthanides (HL) presented a good symmetry. The results showed that the reaction of Ln{sup III} with luminol was a spontaneous process by the electrostatic force with the association constants K at 10{sup 6}–10{sup 7} level. The binding constants K{sub D} (about 10{sup 4}–10{sup 5}) and the number of binding sites n (about 1) of Ln{sup III} (La{sup III}, Eu{sup III}, Gd{sup III}, Tb{sup III} and Lu{sup III}) to bovine serum albumin were first given by FI–CL, and the binding ability of Ln{sup III} followed the pattern: La{sup III}

  14. Molecular beam chemiluminescence studies of the NO + O/sub 3/ reaction and modeling of global NO/sub 2/ distribution

    Energy Technology Data Exchange (ETDEWEB)

    Kowalczyk, M.

    1980-11-01

    The results of a crossed molecular beam study of the chemiluminescent reaction NO + 0/sub 3/ ..-->.. NO/sub 2/ + 0/sub 2/ are discussed. The chemiluminescence as a function of collision energy and an excitation function were obtained using a translationally cooled supersonic NO beam. An investigation into the role of the internal energy states using an effusive NO beam and a supersonic O/sub 3/ beam has been presented. The results show that chemiluminescence enhancement occurs when high and low temperature NO experiments are compared. The role that other energy modes may have is discussed. The observed enhancement is consistent with the concept that the chemiluminescence cross section increases with NO molecular rotation for low J states. The second part discusses the role of NO/sub 2/ in preserving a global ozone balance. NO/sub 2/ vertical profiles based on Noxon's (1979) column measurements were derived. The method of instantaneous rates was used to calculate the rate of ozone production and destruction by O/sub x/ and NO/sub x/ on a grid that covered the entire globe. The results were presented as a function of altitude and latitude in contour plots.

  15. High chemiluminescence activity of an Fe(III)-TAML activator in aqueous-organic media and its use in the determination of organic peroxides.

    Science.gov (United States)

    Demiyanova, Alexandra S; Sakharov, Ivan Yu

    2015-05-07

    High activity of Fe(III)-TAML, peroxidase mimic, upon the catalytic oxidation of luminol in aqueous-organic media (ethanol, isopropanol and acetonitrile) was determined. Using Fe(III)-TAML the sensitive chemiluminescence assays for the determination of benzoyl peroxide and tert-butyl hydroperoxide in the presence of organic solvents were performed.

  16. Solid-state chemiluminescence assay for ultrasensitive detection of antimony using on-vial immobilization of CdSe quantum dots combined with liquid–liquid–liquid microextraction

    Energy Technology Data Exchange (ETDEWEB)

    Costas-Mora, Isabel; Romero, Vanesa; Lavilla, Isela; Bendicho, Carlos, E-mail: bendicho@uvigo.es

    2013-07-25

    Graphical abstract: -- Highlights: •Solid-state chemiluminescence based on CdSe QDs was developed. •QDs immobilization in a vial was achieved in a simple and fast way. •Antimony detection was achieved by inhibition of the CdSe QDs/H{sub 2}O{sub 2} CL reaction. •LLLME allowed improving the selectivity and sensitivity of the CL assay. •The capping ligand played a critical role in the selectivity of the CL system. -- Abstract: On-vial immobilized CdSe quantum dots (QDs) are applied for the first time as chemiluminescent probes for the detection of trace metal ions. Among 17 metal ions tested, inhibition of the chemiluminescence when CdSe QDs are oxidized by H{sub 2}O{sub 2} was observed for Sb, Se and Cu. Liquid–liquid–liquid microextraction was implemented in order to improve the selectivity and sensitivity of the chemiluminescent assay. Factors influencing both the CdSe QDs/H{sub 2}O{sub 2} chemiluminescent system and microextraction process were optimized for ultrasensitive detection of Sb(III) and total Sb. In order to investigate the mechanism by which Sb ions inhibit the chemiluminescence of the CdSe QDs/H{sub 2}O{sub 2} system, atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), UV–vis absorption and fluorescence measurements were performed. The selection of the appropriate CdSe QDs capping ligand was found to be a critical issue. Immobilization of QDs caused the chemiluminescence signal to be enhanced by a factor of 100 as compared to experiments carried out with QDs dispersed in the bulk aqueous phase. Under optimized conditions, the detection limit was 6 ng L{sup −1} Sb and the repeatability expressed as relative standard deviation (N = 7) was about 1.3%. An enrichment factor of 95 was achieved within only 3 min of microextraction. Several water samples including drinking, spring, and river waters were analyzed. The proposed method was validated against CRM NWTM-27.2 fortified lake water, and a recovery study was

  17. Measurements in international units of antibody to hepatitis B surface antigen(anti-HBs) after immunization with a yeast-derived, subtype adr hepatitis B vaccine are considerably different between chemiluminescent immunoassay (CLIA) and chemiluminescent enzyme immunoassay (CLEIA).

    Science.gov (United States)

    Ogata, Norio

    2006-04-01

    The worldwide consensus of the minimum protective anti-HBs level against HBV infection is 10 mIU/mL on assays standardized by the World Health Organization (WHO) reference preparations. To investigate whether this value could be applied to recipients of yeast-derived recombinant HB vaccine containing the major surface protein of subtype adr (Bimmugen, Astellas Pharmaceutical, Tokyo), we compared anti-HBs measurements between chemiluminescent immunoassay (CLIA) (Architect Ausab, Abbott Japan, Tokyo) and chemiluminescent enzyme immunoassay (CLEIA) (Lumipulse Forte, Fujirebio, Tokyo) in given serum samples obtained from the vaccinees. The vaccine and the two assay methods are currently in a wide use in Japan. The study included 300 medical students who completed a standard vaccination course (0, 1 and 6 months). Serum samples obtained 1 month or 13 months after completing the vaccination were simultaneously tested for anti-HBs by CLIA and CLEIA. In 147 samples with quantifiable values on both CLIA and CLEIA (10 to 1000 mIU/mL) the geometric mean titer on CLEIA (225.0 mIU/mL) was significantly higher than that on CLIA (94.5 mIU/mL) (p anti-HBs measurements compared to CLIA and discordance in determining critical anti-HBs level of 10 mIU/mL was observed in more than half the samples. This suggests that the minimum HBV-protective anti HBs titer of 10 mIU/mL is difficult to be introduced to Japan where subtype adr-HB vaccines or -HBV infection are prevalent, unless characteristics of assay methods are carefully evaluated.

  18. Electrogenerated chemiluminescence of BODIPY, Ru(bpy)3(2+), and 9,10-diphenylanthracene using interdigitated array electrodes.

    Science.gov (United States)

    Nepomnyashchii, Alexander B; Kolesov, G; Parkinson, B A

    2013-07-10

    Interdigitated array electrodes (IDAs) were used to produce steady-state electrogenerated chemiluminescence (ECL) by annihilation of oxidized and reduced forms of a substituted boron dipyrromethene (BODIPY) dye, 9,10-diphenylanthracene (DPA), and ruthenium(II) tris(bypiridine) (Ru(bpy)3(2+)). Digital simulations were in good agreement with the experimentally obtained currents and light outputs. Coreactant experiments, using tri-n-propylamine and benzoyl peroxide as a sacrificial homogeneous reductant or oxidant, show currents corresponding to electrode reactions of the dyes and not the oxidation or reduction of the coreactants. The results show that interdigitated arrays can produce stable ECL where the light intensity is magnified due to the larger currents as a consequence of feedback between generator and collector electrodes in the IDA. The light output for ECL is around 100 times higher than that obtained with regular planar electrodes with similar area.

  19. A biostatistical study into the efficiency of individualism using nonisotopic chemiluminescent-enhanced NICE multilocus DNA probes.

    Science.gov (United States)

    Hau, P P; Watt, E H; Hau, C M

    1997-10-01

    The efficiency of individualisation using nonisotopic chemiluminescent- enhanced probes (NICE) was investigated by analysing DNA fingerprints obtained from 190 unrelated Caucasians. Novel analysis of the scoring procedure enabled us to include the comparison of 585 pairs of samples for each of two probes. When the results of NICE probes 33.6 and 33.15 were combined, the mean percentage band share between two unrelated individuals was 16.8% and the mean number of bands identified in an individual DNA fingerprint was 54.8. Results were compared with those obtained using isotopically labelled probes and suggest that the two labelling systems gave similar efficiencies for differentiating between individuals. Analysis of DNA fingerprints from 37 family trios (mother, child and father groups) gave a mutation rate of 0.10% when using NICE probes. The two labelling systems compared were equally efficient in establishing family relationships.

  20. Improvement on Simultaneous Determination of Cr(III) and Cr(VI) by Capillary Electrophoresis and Chemiluminescence Detection

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    A sensitive method for the simultaneous determination of Cr(III) and Cr(VI) using in-capillary reaction capillary electrophoresis separation and chemiluminescence detection was developed. The procedures were designed as follows: The sample, hydrochloric acid and sodium hydrogen sulfite solution segments were injected sequentially into the capillary. The reaction of Cr(VI) reduced to Cr(III) by HSO3- occurred inside the capillary after applying the running voltage. According to the migration time difference of both Cr(III) ions moving towards to the cathode (detection end), they could be separated and determined. The limits of detection for chromium(III) and chromium(VI) (S/N = 3) were 6.0(10-13 mol/L (12 zmol) and 1.9(10-11 mol/L (380 zmol), respectively.

  1. Modulatory effects of non-steroidal anti-inflammatory drugs on the luminol and lucigenin amplified chemiluminescence of equine neutrophils.

    Science.gov (United States)

    Benbarek, H; Ayad, A; Deby-Dupont, G; Boukraa, L; Serteyn, D

    2012-03-01

    The purpose of this study was to explore the potential modulation of equine neutrophil oxidative burst by a series of classical NSAIDs which was subsequently monitored by the luminol or lucigenin-enhanced chemiluminescence (CL) technique. A significant dose-dependent inhibition of the luminol CL was observed with the majority of investigated drugs. This inhibition was very significant for phenylbutazone and Indomethacin; while for aspirin, a higher concentration is required. The action of Ketoprofen was significant during the first 5 min and only when the concentration was above 1 mM. Indomethacin and acetylsalicylic acid result in an inhibition dose-dependent of luminol CL. On the other hand, the phenylbutazone showed an inhibiting effect when used either luminol or lucigenin though luminol is slightly better. When the ketoprofen is considered, an inhibiting effect of luminal CL was observed but less significant than the other NSAIDs investigated. The flunixin meglumine enhances strongly the CL.

  2. Chemiluminescence-imaging detection of DNA on a solid-phase membrane by using a peroxidase-labeled macromolecular probe.

    Science.gov (United States)

    Azam, Md Golam; Yamasuji, Mutsumi; Krawczyk, Tomasz; Shibata, Takayuki; Kabashima, Tsutomu; Kai, Masaaki

    2015-07-01

    We have developed a novel method for sensitive chemiluminescence (CL)-imaging detection of DNA by using a macromolecular probe synthesized by attaching multiple molecules of horseradish peroxidase (HRP) and biotin in dextran backbone. The probe formed a macromolecular assembly by binding to streptavidin which specifically recognized biotinylated complementary DNA, which was hybridized to a target DNA on a solid-phase membrane. This methodology was applied to CL-imaging detection of a synthetic telomere DNA (TTAGGG)10 and human telomere DNA by using the CL probe comprising of dextranT2000 (MW=ca. 2000kDa) bonded to approximately 42 molecules of HRP and 210 molecules of biotin. The human telomere DNA in a small number of buccal mucous cells (ca. 70 cell numbers) of cheek tissue was quantitatively determined by the proposed CL detection method that afforded approximately 10 times higher sensitivity than that of the conventional CL method using commercially available HRP-avidin probe.

  3. Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting

    Directory of Open Access Journals (Sweden)

    Luis Izquierdo

    2013-11-01

    Full Text Available The disappearance of lytic, protective antibodies (Abs from the serum of patients with Chagas disease is accepted as a reliable indicator of parasitological cure. The efficiency of a chemiluminescent enzyme-linked immunosorbent assay based on a purified, trypomastigote-derived glycosylphosphatidylinositol-anchored mucin antigen for the serologic detection of lytic Abs against Trypanosoma cruzi was evaluated in a nonendemic setting using a panel of 92 positive and 58 negative human sera. The technique proved to be highly sensitive {100%; 95% confidence interval (CI = 96-100} and specific (98.3%; 95% CI = 90.7-99.7, with a kappa score of 0.99. Therefore, this assay can be used to detect active T. cruzi infection and to monitor trypanosomicidal treatment.

  4. Flow—injection Chemiluminescence Sensor for the Determination of Gallic Acid by Immobilizing Luminol and Periodate on Anionexchange Resin

    Institute of Scientific and Technical Information of China (English)

    鞠Huang先; 张四纯; 周国俊

    2002-01-01

    A novel chemiluminescence (CL) sensor for the determination of gallic acld combined with folw injection analysis was developed by electrostatically immobilizing hminol and periodate on anion-exchange resins respectively.Gallic acid was sensed by its enhancing effect on the weak CL reaction between luminol and periodate,which were eluted from the ion exchange column.The possible reaction mechanism of the CL system was suggested and discussed.The response of the sensor to gallic acid concentration was linear over the range of 8.0×10-—1.0×10-6mol/L with a detection limit of 6.5×10-9 mol/L(3σ).The relative standard deviation (RSD) for 7 repetitive determinations of gallic acid(1.0×10-7mol/L) was 1.8?The sensor could be used for over 400 times determination with a good reproducibility.

  5. Chemiluminescence Resonance Energy Transfer Competitive Immunoassay Employing Hapten-Functionalized Quantum Dots for the Detection of Sulfamethazine.

    Science.gov (United States)

    Ma, Mingfang; Wen, Kai; Beier, Ross C; Eremin, Sergei A; Li, Chenglong; Zhang, Suxia; Shen, Jianzhong; Wang, Zhanhui

    2016-07-20

    We describe a new strategy for using chemiluminescence resonance energy transfer (CRET) by employing hapten-functionalized quantum dots (QDs) in a competitive immunoassay for detection of sulfamethazine (SMZ). Core/multishell QDs were synthesized and modified with phospholipid-PEG. The modified QDs were functionalized with the hapten 4-(4-aminophenyl-sulfonamido)butanoic acid. The CRET-based immunoassay exhibited a limit of detection for SMZ of 9 pg mL(-1), which is >4 orders of magnitude better than a homogeneous fluorescence polarization immunoassay and is 2 orders of magnitude better than a heterogeneous enzyme-linked immunosorbent assay. This strategy represents a simple, reliable, and universal approach for detection of chemical contaminants.

  6. Control of excitation and quenching in multi-colour electrogenerated chemiluminescence systems through choice of co-reactant.

    Science.gov (United States)

    Barbante, Gregory J; Kebede, Noah; Hindson, Christopher M; Doeven, Egan H; Zammit, Elizabeth M; Hanson, Graeme R; Hogan, Conor F; Francis, Paul S

    2014-10-20

    We demonstrate a new approach to manipulate the selective emission in mixed electrogenerated chemiluminescence (ECL) systems, where subtle changes in co-reactant properties are exploited to control the relative electron-transfer processes of excitation and quenching. Two closely related tertiary-amine co-reactants, tri-n-propylamine and N,N-diisopropylethylamine, generate remarkably different emission profiles: one provides distinct green and red ECL from [Ir(ppy)3] (ppy=2-phenylpyridinato-C2,N) and a [Ru(bpy)3](2+) (bpy=2,2'-bipyridine) derivative at different applied potentials, whereas the other generates both emissions simultaneously across a wide potential range. These phenomena can be rationalized through the relative exergonicities of electron-transfer quenching of the excited states, in conjunction with the change in concentration of the quenchers over the applied potential range.

  7. Liquid chromatography of antihistamines using post-column tris(2,2'-bipyridine) ruthenium(III) chemiluminescence detection.

    Science.gov (United States)

    Holeman, J A; Danielson, N D

    1994-09-23

    The separation and detection of five antihistamine drugs commonly found within over-the-counter allergy and cold pharmaceutical products was performed by HPLC with chemiluminescence (CL) detection. Comparable detection limits at 5-10 pmol were found for the antihistamines by both UV at 214 nm and tris(2,2'-bipyridine) ruthenium(III) CL. However, urine samples were found not to generate as large an unretained peak by CL detection as compared to those peaks by UV detection at 214 and 254 nm. For example, the pheniramine peak representing 0.15 microgram/ml was almost totally obscured at 214 nm. Quantitative results received for three antihistamine commercial samples ranged from 4 to 8% error in accuracy when an internal standard was used to compensate for short term detector drift.

  8. Simultaneous Determination of Size and Quantification of Gold Nanoparticles by Direct Coupling Thin layer Chromatography with Catalyzed Luminol Chemiluminescence

    Science.gov (United States)

    Yan, Neng; Zhu, Zhenli; He, Dong; Jin, Lanlan; Zheng, Hongtao; Hu, Shenghong

    2016-04-01

    The increasing use of metal-based nanoparticle products has raised concerns in particular for the aquatic environment and thus the quantification of such nanomaterials released from products should be determined to assess their environmental risks. In this study, a simple, rapid and sensitive method for the determination of size and mass concentration of gold nanoparticles (AuNPs) in aqueous suspension was established by direct coupling of thin layer chromatography (TLC) with catalyzed luminol-H2O2 chemiluminescence (CL) detection. For this purpose, a moving stage was constructed to scan the chemiluminescence signal from TLC separated AuNPs. The proposed TLC-CL method allows the quantification of differently sized AuNPs (13 nm, 41 nm and 100 nm) contained in a mixture. Various experimental parameters affecting the characterization of AuNPs, such as the concentration of H2O2, the concentration and pH of the luminol solution, and the size of the spectrometer aperture were investigated. Under optimal conditions, the detection limits for AuNP size fractions of 13 nm, 41 nm and 100 nm were 38.4 μg L‑1, 35.9 μg L‑1 and 39.6 μg L‑1, with repeatabilities (RSD, n = 7) of 7.3%, 6.9% and 8.1% respectively for 10 mg L‑1 samples. The proposed method was successfully applied to the characterization of AuNP size and concentration in aqueous test samples.

  9. Chemiluminescence immunoassay based on dual signal amplification strategy of Au/mesoporous silica and multienzyme functionalized mesoporous silica

    Energy Technology Data Exchange (ETDEWEB)

    Lin Jiehua, E-mail: linjiehua@qust.edu.cn [Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042 (China); Zhao Yue; Wei Zhijing; Wang Wei [Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042 (China)

    2011-11-15

    Highlights: > The increased amount of monoclonal antibody in Au/SiO{sub 2} led to a wider linear range. > Due to the increased HRP tags in HRP-Ab{sub 2}/SiO{sub 2}, signal amplification achieved. > A simple dual amplification immunoassay achieved with flow injection analysis. - Abstract: A chemiluminescent dual signal amplification strategy for the determination of {alpha}-fetoprotein (AFP) was proposed based on a sandwich immunoassay format. Monoclonal antibody of AFP immobilized on the gold nanoparticles doped mesoporous SiO{sub 2} (Au/SiO{sub 2}) were prepared and used as a primary antibody. Horseradish peroxidase (HRP) and HRP-labeled secondary antibody (Ab{sub 2}) co-immobilized into the mesoporous SiO{sub 2} nanoparticles (HRP-Ab{sub 2}/SiO{sub 2}) were used as the labeled immunological probe. Due to the high ratio surface areas and pore volumes of the mesoporous SiO{sub 2}, not only the amount of AFP monoclonal antibody but also the amount of the modified HRP and Ab{sub 2} in HRP-Ab{sub 2}/SiO{sub 2} were largely increased. Thus the chemiluminescent signal was amplified by using the system of luminol and H{sub 2}O{sub 2} under the catalysis of HRP. Under the optimal conditions, two linear ranges for AFP were obtained from 0.01 to 0.5 ng mL{sup -1} and 0.5 to 100 ng mL{sup -1} with a detection limit of 0.005 ng mL{sup -1} (3{sigma}). The fabricated signal amplification strategy showed an excellent promise for sensitive detection of AFP and other tumor markers.

  10. Development of a highly sensitive chemiluminescent assay for hydrogen peroxide under neutral conditions using acridinium ester and its application to an enzyme immunoassay.

    Science.gov (United States)

    Arakawa, Hidetoshi; Tsuruoka, Keiko; Ohno, Ken-ichi; Tajima, Noriko; Nagano, Hiromi

    2014-06-01

    We developed a highly sensitive chemiluminescent (CL) assay for hydrogen peroxide using 10-methyl-9-(phenoxycarbonyl) acridinium fluorosulfonate (PMAC) that produced chemiluminescence under neutral conditions and applied it to an enzyme immunoassay (EIA). One picomole of hydrogen peroxide could be detected using the optimized PMAC-CL method and 6.2 × 10(-20) mol β-D-galactosidase (β-gal) could be detected by combining an indoxyl derivative substrate and the proposed PMAC-CL method. This highly sensitive CL β-gal assay was applied to an EIA for thyroid-stimulating hormone (TSH) using β-gal as a label enzyme; 0.02-100.0 μU/mL TSH in human serum could be assayed directly and with high reproducibility.

  11. Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III-Tetrakis (4-Sulfonatophenyl Porphyrin-Luminol-Hydrogen Peroxide System

    Directory of Open Access Journals (Sweden)

    Sayed Yahya Kazemi

    2012-01-01

    Full Text Available The present work deals with an attempt to study the effect of human and bovine serum albumin on kinetic parameters of chemiluminescence of luminol-hydrogen peroxide system catalyzed by manganese tetrasulfonatophenyl porphyrin (MnTSPP. The investigated parameters involved pseudo-first-order rise and fall rate constant for the chemiluminescence burst, maximum level intensity, time to reach maximum intensity, total light yield, and values of the intensity at maximum CL which were evaluated by nonlinear least square program KINFIT. Because of interaction of metalloporphyrin with proteins, the CL parameters are drastically affected. The systems resulted in Stern-Volmer plots with values of 3.17×105 and 3.7×105M−1 in the quencher concentration range of 1.5×10−6 to 1.5×10−5 M for human serum albumin (HSA and bovine serum albumin (BSA, respectively.

  12. High performance liquid chromatography with immobilized Ru(bpy)32+-KMn04 chemiluminescence detection and its application in metabolism of repaglinide in pig liver microsomes

    Institute of Scientific and Technical Information of China (English)

    Ai Hua Fu; Zhu Jun Zhang; Li Li Chen; Xiao Ming Zhang; Pan Xue

    2011-01-01

    A novel high performance liquid chromatography-chemiluminescence (HPLC-CL) method for investigation of in vitro metabolism of repaglinide in pig liver microsomes with microdialysis sampling technique was developed. The chromatographic separation was performed on a Hypersil BDS-C18 column with an isocratic mobile phase consisting of methanol and 0.01 mol/L KH2PO4 (pH 3.0) (volume ratio 75:25) at a flow rate of 1.0 mL/min. The detection was based on the chemiluminescence reaction of repaglinide with acidic potassium permanganate (KMnO4) and tris (2,2'-bipyridyl)ruthenium(III) (Ru(bpy)33+), which was immobilized on the cationic ion-exchange resin for obtaining high sensitivity and reducing consumption of expensive reagent.

  13. Chemiluminescent methods and instruments for monitoring of the atmosphere and satellite validation on board of research aircrafts and unmanned aerial vehicles

    Science.gov (United States)

    Sitnikov, Nikolay; Borisov, Yuriy; Akmulin, Dimitry; Chekulaev, Igor; Sitnikova, Vera; Ulanovsky, Alexey; Sokolov, Alexey

    The results of development of instruments based on heterophase chemiluminescence for measurements of space distribution of ozone and nitrogen oxides concentrations on board of research aircrafts and unmanned aerial vehicles carried out in Central Aerological Observatory are presented. Some results of atmospheric investigations on board of research aircrafts M55 “Geophysica” (Russia) and “Falcon” (Germany) carried out using developed instruments in frame of international projects are demonstrated. Small and low power instruments based on chemiluminescent principle for UAV are developed. The results of measurements on board of UAV are shown. The development can be used for satellite data validation, as well as operative environmental monitoring of contaminated areas in particular, chemical plants, natural and industrial disasters territories, areas and facilities for space purposes etc.

  14. Development and validation of a direct sandwich chemiluminescence immunoassay for measuring DNA adducts of benzo[a]pyrene and other polycyclic aromatic hydrocarbons

    DEFF Research Database (Denmark)

    Georgiadis, Panagiotis; Kovács, Katalin; Kaila, Stella;

    2012-01-01

    We have developed and validated a sandwich chemiluminescence immunoassay (SCIA) which measures polycyclic aromatic hydrocarbon (PAH)-DNA adducts combining high throughput and adequate sensitivity, appropriate for evaluation of adduct levels in human population studies. Fragmented DNA is incubated...... with rabbit antiserum elicited against DNA modified with r7,t8-dihydroxy-t-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) and subsequently trapped by goat anti-rabbit IgG bound to a solid surface. Anti-single-stranded (ss) DNA antibodies binds in a quantity proportional to the adduct levels...... and is detected by chemiluminescence. The BPDE-DNA SCIA has a limit of detection of 3 adducts per 10(9) nucleotides with 5 µg DNA per well. We have validated the BPDE-DNA SCIA using DNA modified in vitro, DNA from benzo[a]pyrene (BP)-exposed cultured cells and mice. The levels of adduct measured by SCIA were...

  15. Radiation-Induced Graft Polymerization of Vinyl Monomers with Anion Groups onto MWNT Supports and Their Application as Electrogenerated Chemiluminescence (ECL Biosensors

    Directory of Open Access Journals (Sweden)

    Ji-Hye Park

    2014-01-01

    Full Text Available Vinyl polymer-grafted multiwalled carbon nanotube (MWNT supports with anion groups were prepared for use as biosensor supports by radiation-induced graft polymerization (RIGP of the vinyl monomers acryloyl diphosphoric acid (ADPA, acrylic acid (AA, sodium styrenesulfonate (NaSS, and methacrylic acid (MA onto the surface of MWNTs. The electrogenerated chemiluminescence sensors based on a glass carbon electrode (ECL-GCE and a screen printed electrode (ECL-SPE were fabricated by immobilization of Ru(bpy3 2+ complex after coating of vinyl polymer-grafted MWNT inks on the surface of the GCE and SPE without any polymer binders in order to obtain high electrogenerated chemiluminescence intensity. For detection of alcohol concentration, alcohol dehydrogenase (ADH was immobilized onto an ECL-GCE sensor prepared by poly(NaSS-g-MWNT supports. The prepared biosensor based on ADH is suitable for the detection of ethanol concentration in commercial drinks.

  16. СHANGES IN PARAMETERS OF LUMINOL-DEPENDENT AND LUCIGENIN-DEPENDENT CHEMILUMINESCENCE OF PERIPHERAL BLOOD NEUTROPHILS IN PATIENTS WITH BLADDER CANCER IN THE DISEASE DYNAMICS

    Directory of Open Access Journals (Sweden)

    L. M. Kurtasova

    2015-01-01

    Full Text Available The study deals with parameters of luminol-dependent and lucigenin-dependent chemiluminescence (CL of peripheral blood neutrophils from patients with bladder cancer (BC prior to surgical treatment. We examined sixty patients (45 to 55 years old with advanced bladder cancer (TNM prior to the operation, and forty-six patients at 10 days after surgical treatment. A control group consisted of 56 healthy donors. Luminol-dependent and lucigenin-dependent chemiluminescence of blood neutrophils was assessed according to De Sole et al. (1983. Chemiluminescence assays of peripheral blood neutrophils from the patients with bladder cancer revealed changes in production of reactive oxygen species (ROS, both for initial stage of oxidation reaction, and total level of active oxygen radicals. We have found disturbed values of primary-to-secondary ROS ratio in the cells. In the patients with bladder cancer, some changes in oxidative metabolism of the blood neutrophils have been registered. These alterations may play an important role in promotion of potential effector cell functions, thus, probably, affecting the whole-scale development of a cytopathic effect exerted by neutrophilic granulocytes. 

  17. Determination of the pseudoephedrine content in pharmaceutical formulations and in biological fluids using a microbore HPLC system interfaced to a microfluidic chemiluminescence detector.

    Science.gov (United States)

    Kadavilpparampu, Afsal Mohammed; Al-Lawati, Haider A J; Suliman, FakhrEldin O; Al Kindy, Salma M Z

    2015-12-01

    A novel automated precolumn derivatization followed by separation using liquid chromatography for the determination of pseudoephedrine (PSE) by a microfluidic chemiluminescence detector has been developed. An on-line derivatization procedure was utilized by converting PSE into a highly light emitting species in a Ru(bipy)3(2+)-peroxydisulphate chemiluminescence (CL) system by derivatizing it with a 1.0 M formaldehyde solution. The derivatized analyte was directly injected into a microbore high-performance liquid chromatography (HPLC) system coupled to an on-chip chemiluminescence detector. The newly developed highly selective, sensitive and fast HPLC-CL method was validated and successfully applied for the analysis of PSE in pharmaceutical formulations and a human urine sample. The selectivity of the method is not only due to the HPLC separation but is also due to the highly selective detection principle of the Ru(bipy)3(2+)-peroxydisulphate CL system used. There was no interference observed from the common preservatives and excipients used in pharmaceutical preparations, which did not show any significant CL signal. The retention time of PSE was less than 3 min, and the detection limits and quantification limits were found to be 5.7 and 26.0 µg L(-1), respectively.

  18. On-Chip Isothermal Nucleic Acid Amplification on Flow-Based Chemiluminescence Microarray Analysis Platform for the Detection of Viruses and Bacteria.

    Science.gov (United States)

    Kunze, A; Dilcher, M; Abd El Wahed, A; Hufert, F; Niessner, R; Seidel, M

    2016-01-01

    This work presents an on-chip isothermal nucleic acid amplification test (iNAAT) for the multiplex amplification and detection of viral and bacterial DNA by a flow-based chemiluminescence microarray. In a principle study, on-chip recombinase polymerase amplification (RPA) on defined spots of a DNA microarray was used to spatially separate the amplification reaction of DNA from two viruses (Human adenovirus 41, Phi X 174) and the bacterium Enterococcus faecalis, which are relevant for water hygiene. By establishing the developed assay on the microarray analysis platform MCR 3, the automation of isothermal multiplex-amplification (39 °C, 40 min) and subsequent detection by chemiluminescence imaging was realized. Within 48 min, the microbes could be identified by the spot position on the microarray while the generated chemiluminescence signal correlated with the amount of applied microbe DNA. The limit of detection (LOD) determined for HAdV 41, Phi X 174, and E. faecalis was 35 GU/μL, 1 GU/μL, and 5 × 10(3) GU/μL (genomic units), which is comparable to the sensitivity reported for qPCR analysis, respectively. Moreover the simultaneous amplification and detection of DNA from all three microbes was possible. The presented assay shows that complex enzymatic reactions like an isothermal amplification can be performed in an easy-to-use experimental setup. Furthermore, iNAATs can be potent candidates for multipathogen detection in clinical, food, or environmental samples in routine or field monitoring approaches.

  19. Review on application of chemiluminescent detection for microfluidic chip%化学发光检测在微流控芯片中的应用综述

    Institute of Scientific and Technical Information of China (English)

    奚忠华; 许丹科

    2012-01-01

    A review is provided of the application of chemiluminescent detection for microfluidic chip in recent years. It is pointed out that microfluidic chip (also known as "lab on a chip" or "micro total analysis system") has increasingly attracted attention in the past two decades, due to its advantages including miniaturization, integration, and automation. Besides, it is also suggested that chemiluminescence detection has the advantages of simple facility, low background noise, and low costs for operation and maintenance. This makes it feasible to adopt chemiluminescence detection as a suitable detection technique for microfluidic chip.%综述了近年来化学发光检测在微流控芯片中的应用.指出微流控芯片(又称为“芯片实验室”或者“微型全分析系统”)因具有小型化、集成化和自动化等特点而在近20年来日益受到关注,而化学发光检测具有仪器结构简单、背景噪音低、操作和维护成本低等优点,非常适合用作微流控芯片的检测手段.

  20. CdTe quantum dots@luminol as signal amplification system for chrysoidine with chemiluminescence-chitosan/graphene oxide-magnetite-molecularly imprinting sensor.

    Science.gov (United States)

    Duan, Huimin; Li, Leilei; Wang, Xiaojiao; Wang, Yanhui; Li, Jianbo; Luo, Chuannan

    2016-01-15

    A sensitive chemiluminescence (CL) sensor based on chemiluminescence resonance energy transfer (CRET) in CdTe quantum dots@luminol (CdTe QDs@luminol) nanomaterials combined with chitosan/graphene oxide-magnetite-molecularly imprinted polymer (Cs/GM-MIP) for sensing chrysoidine was developed. CdTe QDs@luminol was designed to not only amplify the signal of CL but also reduce luminol consumption in the detection of chrysoidine. On the basis of the abundant hydroxy and amino, Cs and graphene oxide were introduced into the GM-MIP to improve the adsorption ability. The adsorption capacities of chrysoidine by both Cs/GM-MIP and non-imprinted polymer (Cs/GM-NIP) were investigated, and the CdTe QDs@luminol and Cs/GM-MIP were characterized by UV-vis, FTIR, SEM and TEM. The proposed sensor can detect chrysoidine within a linear range of 1.0×10(-7) - 1.0×10(-5) mol/L with a detection limit of 3.2×10(-8) mol/L (3δ) due to considerable chemiluminescence signal enhancement of the CdTe quantum dots@luminol detector and the high selectivity of the Cs/GM-MIP system. Under the optimal conditions of CL, the CdTe QDs@luminol-Cs/GM-MIP-CL sensor was used for chrysoidine determination in samples with satisfactory recoveries in the range of 90-107%.

  1. An application of Ag(III) complex chemiluminescence system for the determination of enoxacin in capsule and biological fluid.

    Science.gov (United States)

    Chen, Peiyun; Sun, Hanwen

    2010-01-01

    Ag(III) complex chemiluminescence (CL) system was applied for the determination of enoxacin (ENX). The CL conditions of [Ag(HIO(6))(2)](5-)-H(2)SO(4)-ENX systems without any luminescence reagent were investigated and optimized. Under the optimized conditions, the CL intensity was proportional to the concentration of ENX in the range from 6.6 × 10(-5) to 3.3 × 10(-3) g/L. The limit of detection (s/n = 3) was 2.0 × 10(-5) g/L. The recovery of ENX from the spiked pharmaceutical preparations was in the range of 82.9-108% with a relative standard deviation of 1.9-3.0%. For spiked serum and urine samples the recovery of ENX was in the range of 83.7-110% with a relative standard deviation of 1.1-2.8%. The proposed method was applied successfully to the determination of the drug in capsule, serum and urine samples.

  2. Simultaneous determination of amiodarone and its metabolite desethylamiodarone by high-performance liquid chromatography with chemiluminescent detection

    Energy Technology Data Exchange (ETDEWEB)

    Perez-Ruiz, Tomas [Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia (Spain)], E-mail: tpr@um.es; Martinez-Lozano, Carmen; Garcia-Martinez, Maria Dolores [Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia (Spain)

    2008-08-08

    A novel method was developed for the determination of amiodarone and desethylamiodarone by high-performance liquid chromatography (HPLC) coupled with chemiluminescent (CL) detection. The procedure is based on the post-column photolysis of the analytes into photoproducts which are active in the tris(2,2'-bipyridyl)ruthenium(III) [Ru(bpy){sub 3}{sup 3+}] CL system. Ru(bpy){sub 3}{sup 3+} was on-line generated by photo-oxidation of the Ru(II) complex in the presence of peroxydisulfate. The separation was carried out on a Mediterranea C{sub 18} column with isocratic elution using a mixture of methanol and 0.017 mol L{sup -1} ammonium sulfate buffer of pH 6.8. Under the optimum conditions, analytical curves, based on standard solutions, were linear over the range 0.1-50 {mu}g mL{sup -1} for amiodarone and 0.5-25 {mu}g mL{sup -1} for desethylamiodarone. The detection limits of amiodarone and desethylamiodarone were 0.02 and 0.11 {mu}g mL{sup -1}, respectively. Intra- and inter-day precision values of 0.9% relative standard deviation (R.S.D.) (n = 10) and 1.6% R.S.D. (n = 15), respectively, were obtained. The method was applied successfully to the determination of these compounds in serum and pharmaceutical formulations.

  3. Determination of vitamin K homologues by high-performance liquid chromatography with on-line photoreactor and peroxyoxalate chemiluminescence detection

    Energy Technology Data Exchange (ETDEWEB)

    Ahmed, Sameh [Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521 (Japan); Kishikawa, Naoya [Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521 (Japan); Nakashima, Kenichiro [Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521 (Japan); Kuroda, Naotaka [Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521 (Japan)]. E-mail: n-kuro@nagasaki-u.ac.jp

    2007-05-22

    A sensitive and highly selective high-performance liquid chromatography (HPLC) method was developed for the determination of vitamin K homologues including phylloquinone (PK), menaquinone-4 (MK-4) and menaquinone-7 (MK-7) in human plasma using post-column peroxyoxalate chemiluminescence (PO-CL) detection following on-line ultraviolet (UV) irradiation. The method was based on ultraviolet irradiation (254 nm, 15 W) of vitamin K to produce hydrogen peroxide and a fluorescent product at the same time, which can be determined with PO-CL detection. The separation of vitamin K by HPLC was accomplished isocratically on an ODS column within 35 min. The method involves the use of 2-methyl-3-pentadecyl-1,4-naphthoquinone as an internal standard. The detection limits (signal-to-noise ratio = 3) were 32, 38 and 85 fmol for PK, MK-4 and MK-7, respectively. The recoveries of PK, MK-4 and MK-7 were greater than 82% and the inter- and intra-assay R.S.D. values were 1.9-5.4%. The sensitivity and selectivity of this method were sufficient for clinical and nutritional applications.

  4. A novel method for Co(Ⅱ) and Cu(Ⅱ) analysis by capillary electrophoresis with chemiluminescence detection

    Institute of Scientific and Technical Information of China (English)

    Xiao Ming Guo; Xiang Dong Xu; Hui Jing Zhang; Yong Gang Hu; Jie Zhang

    2007-01-01

    Based on the fact that some metal ions can catalyze the chemiluminescence (CL) reaction of luminol with K3Fe(CN)6, a novel capillary electrophoresis CL method was developed for the determination of Co(Ⅱ) and Cu(Ⅱ). The separation was carried out with a 10 mmol/L sodium acetate solution containing 0.8 mmol/L luminol and 2.0 mmol/L α-HIBA (adjusted to pH 4.8 by Hac solution). The post-capillary reagent was 2.0 mmol/L K3Fe(CN)6 which was adjusted to pH 13.0 by NaOH solution. Under the optimum conditions, the detection limits (S/N = 3) for Co(Ⅱ) and Cu(Ⅱ) were 7.5 × 10-11 mol/L and 7.5 × 10-9 mol/L, with the linear range of 7.5 × 10-9 mol/L to 1.0 × 10-6 mol/L and 7.5 × 10-8 mol/L to 5.0 × 10-5 mol/L,respectively.

  5. Determination of 2-methoxyestradiol by chemiluminescence based on luminol-KMnO4-CdTe quantum dots system.

    Science.gov (United States)

    Du, Bin; Wang, Tiantian; Han, Shuping; Cao, Xiaohui; Qu, Tiantian; Zhao, Feifei; Guo, Xinhong; Yao, Hanchun

    2015-02-05

    In this study, water-soluble CdTe quantum-dots (QDs) capped with glutathione (GSH) was synthesized. It was found that CdTe QDs could greatly enhance the chemiluminescence (CL) emission from the luminol-KMnO4 system in alkaline medium, and 4 nm CdTe QDs was used as catalysts to enhance the reaction sensitivity. The CL intensity of CdTe QDs-luminol-KMnO4 was strongly inhibited in the presence of 2-methoxyestradiol (2-ME) and the relative CL intensity was in linear correlation with the concentration of 2-ME. Based on this inhibition, a novel CL method with a lower detection limit and wider linear range was developed for the determination of 2-ME. The detection limit of plasma samples was 3.07×10(-10) g mL(-1) with a relative standard deviation of 0.24% for 8.0×10(-9) g mL(-1) 2-ME. The method was successfully applied for determination of 2-ME in plasma samples. The possible CL reaction mechanism was also discussed briefly.

  6. Flow injection chemiluminescence sensor based on core-shell magnetic molecularly imprinted nanoparticles for determination of sulfadiazine.

    Science.gov (United States)

    Lu, Fuguang; Li, Huaijiang; Sun, Min; Fan, Lulu; Qiu, Huamin; Li, Xiangjun; Luo, Chuannan

    2012-03-09

    A novel flow injection chemiluminescence (FI-CL) sensor for determination of sulfadiazine (SDZ) using core-shell magnetic molecularly imprinted polymers (MMIPs) as recognition element is developed. Briefly, a hydrophilic MMIPs layer was produced at the surface of Fe(3)O(4)@SiO(2) magnetic nanoparticles (MNPs) via combination of molecular imprinting and reversible stimuli responsive hydrogel. And it provided the MMIPs with excellent adsorption capacity and rapid adsorption rate due to the imprinted sites mostly situated on the surface of MMIPs. Then the prepared SDZ-MMIPs were packed into flow cell to establish a novel FI-CL sensor. The sensor provided a wide linear range for SDZ of 4.0×10(-7) to 1.0×10(-4) mol L(-1) with a detection limit of 1.54×10(-7) mol L(-1). And the relative standard deviation (RSD) for the determination of 1.0×10(-6) mol L(-1) SDZ was 2.56% (n=11). The proposed method was applied to determine SDZ in urine samples and satisfactory results were obtained.

  7. Lucigenin chemiluminescence assay as an adjunctive tool for assessment of various stages of inflammation: A study of quiescent inflammatory cells

    Indian Academy of Sciences (India)

    Knox Van Dyke; Shaily Patel; Val Vallyathan

    2003-02-01

    A simple, fast, precise and biologically relevant toxicity assay for screening cytotoxicity of minerals would have distinct advantages due to its cost benefits and relative savings in time. Furthermore, a bioassay to differentiate acute and chronic in vivo pulmonary reactions could have potential value as predictors of fibrogenicity and pathogenicity. In this study we examined the potential use of lucigenin as a probe to evaluate the correlation between chemiluminescence (CL) generated by alveolar macrophages with the known cytotoxicity and pathogenicity by conventional bioassays. In this study, we used small doses of dust (20 g) to minimize cellular overload and to maintain homeostasis. Crystalline silica a highly fibrogenic dust was used as positive control and results are compared with those for bentonite, kaolin and talc. Among the three minerals compared with silica, bentonite was more reactive (27%) in CL assay and declined sharply compared to other minerals. This sudden decline in bentonite CL is caused by cytotoxicity leading to cell death. CL-induced by talc was comparable to silica and declines slowly. Kaolin on the other hand produced relatively a weaker (25%) CL compared to silica. Our data using relatively low doses of dust suggest that the CL assay may have a better predictive value in cytotoxicity evaluations compared to conventional toxicity assays.

  8. In vitro inhibitory effects of Moringa oleifera leaf extract and its major components on chemiluminescence and chemotactic activity of phagocytes.

    Science.gov (United States)

    Vongsak, Boonyadist; Gritsanapan, Wandee; Wongkrajang, Yuvadee; Jantan, Ibrahim

    2013-11-01

    The ethanol extract of Moringa oleifera Lam. leaves and its major constituents, crypto-chlorogenic acid, quercetin 3-O-glucoside and kaempferol 3-O-glucoside, were investigated on the respiratory burst of human whole blood and isolated human polymorphonuclear leukocytes (PMNs) using a luminol-based chemiluminescence assay. The chemotactic migration of PMNs was also investigated using the Boyden chamber technique. The ethanol extract demonstrated inhibitory activities on the oxidative burst and the chemotactic migration of PMNs. Quercetin 3-O-glucoside, crypto-chlorogenic acid, and kaempferol 3-O-glucoside, isolated from the extract, expressed relatively strong inhibitory activity on the oxidative burst of PMNs with IC50 values of 4.1, 6.7 and 7.0 microM, respectively, comparable with that of aspirin. They also demonstrated strong inhibition of chemotatic migration of PMNs with IC50 values of 9.5, 15.9 and 18.2 microM, respectively. The results suggest that M. oleifera leaves could modulate the immune response of human phagocytes, linking to its ethnopharmacological use as an anti-inflammatory agent. The immunomodulating activity of the plant was mainly due to its major components.

  9. Simultaneous determination of codeine and noscapine by flow-injection chemiluminescence method using N-PLS regression.

    Science.gov (United States)

    Rezaei, B; Khayamian, T; Mokhtari, A

    2009-02-20

    A flow injection chemiluminescent (FI-CL) method has been developed for the simultaneous determination of codeine and noscapine using N-PLS regression. The method is based on the fact that kinetic characteristics of codeine and noscapine are different in the Ru(phen)(3)(2+)-Ce(IV) CL system. In flow injection mode, codeine gives broad peak with the highest CL intensity at 4.4s, whereas the maximum CL intensity of the noscapine appears at about 2.6s. Moreover, the effect of increasing H(2)SO(4) concentration was different on the CL intensity of the compounds. An experimental design, central composite design (CCD), was used to realize the optimized variables such as Ru(II) and Ce(IV) concentrations for the both compounds. At the optimized condition, a three-way data structure (samples, H(2)SO(4) concentration, time) was constructed and followed by N-PLS regression. The number of factors for the N-PLS regression was selected based on the minimum values for the root mean squared error of cross validation (RMSECV). The proposed method is applied to the simultaneous quantification of codeine and noscapine in the pharmaceutical preparations.

  10. Simultaneous Determination of Epinephrine, Noradrenaline and Dopamine in Human Serum Samples by High Performance Liquid Chromatography with Chemiluminescence Detection

    Institute of Scientific and Technical Information of China (English)

    CHEN, Fu-Nan; ZHANG, Ying-Xue; ZHANG, Zhu-Jun

    2007-01-01

    A simple, rapid and accurate high performance liquid chromatographic (HPLC) technique coupled with chemiluminescence (CL) detection was developed for the simultaneous determination of epinephrine (E),noradrenaline (NA) and dopamine (DA). It was based on the analyte enhancement effect on the CL reaction between luminol and potassium ferricyanide. The effects of various parameters, such as potassium ferricyanide concentration, luminol concentration, pH value and component of the mobile phase on chromatographic behaviors of the analytes (E, NA and DA) were investigated. The separation was carried out on C18 column using the mobile tions, E, NA and DA showed good linear relationships in the range of 1×10-8-5×10-6, 5.0×10-9-1.0× 10-6 and 5.0×10-9-1.0×10-6 g/mL respectively. The detection limits for E, NA and DA were 4.0×10-9, 1.0×10-9 and 8.0×10-10 g/mL. The proposed method has been applied successfully to the analysis of E, NA and DA in human serum samples.

  11. Single and multiplexed immunoassays for the chemiluminescent imaging detection of animal glues in historical paint cross-sections.

    Science.gov (United States)

    Sciutto, G; Dolci, L S; Guardigli, M; Zangheri, M; Prati, S; Mazzeo, R; Roda, A

    2013-01-01

    The characterization of the organic components in a complex, multilayered paint structure is fundamental for studying painting techniques and for authentication and restoration purposes. Proteinaceous materials, such as animal glue, are of particular importance since they are widely used as binders, adhesives and for gilding. Even though proteins are usually detected by chromatographic and proteomic techniques, immunological methods represent an alternative powerful approach to protein analysis thanks to the high specificity of antigen-antibody reactions. Our previous studies demonstrated that ovalbumin and casein could be localized in paint cross-sections with high sensitivity and good spatial resolution (i.e. within the single painting layers) by using chemiluminescent (CL) immunochemical microscope imaging. In the present research work, we describe for the first time the immunolocalization of collagen (the main protein of animal glue) in paint cross-sections by CL imaging microscopy. Two different analytical protocols have been developed, allowing either the detection of collagen or the simultaneous detection of collagen and ovalbumin in the same paint sample. The assays were used to detect collagen and ovalbumin in cross-sections from model samples and historical paintings (a wall painting dated to 1773-1774 and a painted wood panel of the Renaissance period) in order to achieve information on paint techniques and past restoration interventions.

  12. Electrogenerated chemiluminescence: An oxidative-reductive mechanism between quinolone antibiotics and tris(2,2'-bipyridyl)ruthenium(II)

    Energy Technology Data Exchange (ETDEWEB)

    Burkhead, Matthew S.; Wang, Heeyoung; Fallet, Marcel [Department of Chemistry, Creighton University, Omaha, NE 68178 (United States); Gross, Erin M. [Department of Chemistry, Creighton University, Omaha, NE 68178 (United States)], E-mail: eringross@creighton.edu

    2008-04-21

    The cyclic voltammetry and electrogenerated chemiluminescent (ECL) reactions of a series of quinolone and fluoroquinolone antibiotics were investigated in a flow injection analysis (FIA) system. 7-Piperazinyl fluoroquinolone antibiotics were found to participate as a coreactant in an oxidative-reductive ECL mechanism with tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy){sub 3}{sup 2+}) as the luminescent reagent. The reaction mechanism was investigated in order to understand and optimize the processes leading to light emission. The optimal conditions included a solution pH {approx}7 at a flow rate of 3.0 mL min{sup -1} with no added organic modifier and application of 1.2 V vs. a Pt quasi-reference electrode (QRE). Fluoroquinolones containing a tertiary distal nitrogen on the piperazine ring, such as enrofloxacin and ofloxacin, reacted to produce more intense ECL than those with a secondary nitrogen, such as ciprofloxacin and norfloxacin. The method linear range, precision, detection limits, and sensitivity for the detection of enrofloxacin and ciprofloxacin were compared to that of tripropylamine. The method was applied to the determination of the ciprofloxacin content in a pharmaceutical preparation. The assay is discussed in terms of its analytical figures of merit, ease of use, speed, accuracy and application to pharmaceutical samples.

  13. Determining the concentration of procalcitonin using a magnetic particles-based chemiluminescence assay for the clinical diagnosis of sepsis.

    Science.gov (United States)

    Qi, Suwen; Li, Qiaoliang; Rao, Wei; Liu, Xinyu; Yin, Li; Zhang, Huisheng

    2013-01-01

    Our objective is to develop an assay based on magnetic particles (MPs) to determine the concentration of procalcitonin (PCT) using a chemiluminescence immunoassay (CLIA). Fluorescein isothiocyanate (FITC) and N-(aminobutyl)-N-(ethylisoluminol) (ABEI) were used to label two different anti-procalcitonin (PCT) monoclonal antibodies. The labeled antibodies, the PCT antigen, and the anti-FITC antibody-coated MPs formed a double-sandwiched immunocomplex. The measured relative light units (RLUs) of ABEI in the substrate solution were directly proportional to the amount of PCT present in the samples. The proposed method was linear to 600 ng/mL with a detection limit of 0.03 ng/mL. The coefficient of variation (CV) was <5% and <6% for the intra- and inter-assay precision, respectively. The average recoveries were between 95 and 107%. The linearity-dilution effect gave a linear correlation coefficient of 0.9912. This proposed assay provided an alternative method to quantitatively measure PCT in serum for the diagnosis of sepsis.

  14. Comprehensive two-dimensional gas chromatography coupled with fast sulphur-chemiluminescence detection: implications of detector electronics.

    Science.gov (United States)

    Blomberg, Jan; Riemersma, Toby; van Zuijlen, Manfred; Chaabani, Hassan

    2004-09-24

    Within the petrochemical industry, there has been a growing interest in methods capable of providing detailed information on the distribution of sulphur-containing compounds in various product streams, going down to the level of separating and quantifying individual sulphur species. Since no single capillary gas chromatographic column is able to perform this separation, a refuge to multi-dimensional separation techniques has to be taken. In this respect, comprehensive two-dimensional gas chromatography (GC x GC) coupled with sulphur chemiluminescence detection (SCD) has shown to be highly promising. It has been suggested, however, that the detector volume of an SCD restricts its potential to keep up with the fast second-dimension separations of contemporary GC x GC. In this paper, we will demonstrate that the lack of speed of the SCD does not originate from its physical dimensions, but is largely determined by the speed of the electronics used. Additionally, some typical examples will be presented to illustrate the potential of GC x GC coupled with fast SCD.

  15. Evaluation of the antioxidant effects of carotenoids from Deinococcus radiodurans through targeted mutagenesis, chemiluminescence, and DNA damage analyses.

    Science.gov (United States)

    Tian, Bing; Xu, Zhenjian; Sun, Zongtao; Lin, Jun; Hua, Yuejin

    2007-06-01

    Deinococcus radiodurans is highly resistant to reactive oxygen species (ROS). The antioxidant effect of carotenoids in D. radiodurans was investigated by using a targeted mutation of the phytoene synthase gene to block the carotenoid synthesis pathway and by evaluating the survival of cells under environmental stresses. The colorless mutant R1DeltacrtB of D. radiodurans failed to synthesize carotenoids, and was more sensitive to ionizing radiation, hydrogen peroxide, and desiccation than the wild type, suggesting that carotenoids in D. radiodurans help in combating environmental stresses. Chemiluminescence analyses showed that deinoxanthin, a major product in the carotenoid synthesis pathway, had significantly stronger scavenging ability on H2O2 and singlet oxygen than two carotenes (lycopene and beta-carotene) and two xanthophylls (zeaxanthin and lutein). Deinoxanthin also exhibited protective effect on DNA. Our findings suggest that the stronger antioxidant effect of deinoxanthin contribute to the resistance of D. radiodurans. The higher antioxidant effect of deinoxanthin may be attributed to its distinct chemical structure which has an extended conjugated double bonds and the presence of a hydroxyl group at C-1' position, compared with other tested carotenoids.

  16. Determination of organothiophosphorus pesticides in water by liquid chromatography and post-column chemiluminescence with cerium(IV).

    Science.gov (United States)

    Catalá-Icardo, Mónica; Lahuerta-Zamora, Luis; Torres-Cartas, Sagrario; Meseguer-Lloret, Susana

    2014-05-01

    A new, fast, selective and sensitive method has been developed for the simultaneous determination of nine organothiophosphorus (OTP) pesticides, namely omethoate, dimethoate, disulfoton-sulfoxide, methidathion, phosmet, malathion, diazinon, pirimiphos-methyl and chlorpyrifos. The pesticides were separated on a Kinetex C18 column by gradient elution with acetonitrile:water. A post-column basic hydrolysis of the pesticides and later a chemiluminescence (CL) reaction with cerium (IV) in acid medium was carried out. Hexadecylpyridinium chloride highly enhanced the CL emission. Under optimized conditions, linearity, precision, limits of detection and quantification, and accuracy were determined. Both selectivity and sensitivity were compared with those obtained with UV detection. In combination with SPE, limits of detection in the range 15-80ng/L and 5-30ng/L were obtained when 250mL and 1000mL of solution were treated, respectively. When applied to 250mL of sample the inter-day precision of the method was between 3.5% and 7.3% and the intra-day precision between 2.9% and 6.0%. The method was applied to determine OTP pesticides in spiked water samples from different origins: irrigation, river, sea, ground, spring, mineral and tap waters, being the percentage of recovery of added amounts near 100% form most of the pesticides.

  17. Determination of Gallic Acid by Flow Injection Analysis Based on LuminoI-AgNO3-Ag NPs Chemiluminescence System

    Institute of Scientific and Technical Information of China (English)

    李世凤; 孙惠敏; 王东; 钱莉; 朱燕; 陶善军

    2012-01-01

    A novel flow injection procedure has been developed for the determination of gallic acid based on the en- hancement function for luminol-AgNO3-Ag NPs chemiluminescence (CL) system by gallic acid. The enhancement mechanism was proposed for the reinforcing effect of the gallic acid on the CL system. The UV-vis absorption spectrum and CL emission spectrum were applied to confirm the mechanism. The method is simple, rapid and sen- sitive with a detection limit of 5 × 10^-10 g.mL-l and a linear range of 8.0 × 10^-10- 1.0 × 10-7 g.mL-1. The relative standard deviation (RSD) is 1.3% for eleven measurements of 5 × 10^-8 g-mL-1 gallic acid. The method has been successfully applied to the determination of gallic acid in Chinese proprietary medicine--Jianmin Yanhou tablets and synthesized samples.

  18. A lab-on-a-chip device for analysis of amlodipine in biological fluids using peroxyoxalate chemiluminescence system.

    Science.gov (United States)

    Al Lawati, Haider A J; Al-Nadabi, Mira M; Varma, Gouri B; Suliman, Fakhr Eldin O; Al-Abri, Hasnaa

    2014-12-01

    A highly sensitive, rapid and economical method for the determination of amlodipine (AM) in biological fluids was developed using a peroxyoxalate chemiluminescence (CL) system in a lab-on-a-chip device. Peroxyoxalate-CL is an indirect type of CL that allows the detection of native fluorophores or compounds derivatized with fluorescent labels. Here, fluorescamine was reacted with AM, and the derivatization product was used in a bis-(2,4,6-trichlorophenyl)oxalate-CL system. Fluorescamine reacts selectively with aliphatic primary amine at neutral or basic pH. As most of the calcium channel blocker and many cardiovascular drugs do not contain primary amine, the developed method is highly selective. The parameters that influenced the CL signal intensity were studied carefully. These included the chip geometry, pH, concentration of reagents used and flow rates. Moreover, we confirmed our previous observation about the effects of imidazole, which is commonly used in the bis-(2,4,6-trichlorophenyl)oxalate-CL system as a catalyst, and found that the signal was significantly improved when imidazole was absent. Under optimized conditions, a calibration curve was obtained with a linear range (10-100 µg/L). The limit of detection was 3 µg/L, while the limit of quantification was 10 µg/L. Finally the method was applied for the determination of AM in biological fluids successfully.

  19. Electrogenerated chemiluminescence of tris (2,2'-bipyridyl)ruthenium(II) ion-exchanged in nafion-silica composite films

    Science.gov (United States)

    Khramov; Collinson

    2000-07-01

    The voltammetry and electrogenerated chemiluminescence (ECL) of tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)3 2+) ion-exchanged in Nafion and Nafion-silica composite materials have been investigated. The major goal of this work was to investigate and develop new materials and immobilization approaches for the fabrication of ECL-based sensors with improved reactivity and long-term stability. Nation-silica composite materials with varying contents of Nation (53-100 wt% relative to silica) were prepared via the two-step acid/base hydrolysis and condensation of tetramethoxysilane. The Nafion doped sols were spin cast on glassy carbon electrodes, dried, and then ion-exchanged with Ru(bpy)3 2+. The shapes of the cyclic voltammetric curves and the amount of Ru(bpy)3 2+ exchanged into the films strongly depends on the amount of Nafion incorporated into the hybrid sol. Nafion-silica films with a low content of Nafion ion-exchanged less Ru(bpy)3 2+ and exhibited tail-shaped voltammetry at 100 mV/s. The ECL of immobilized Ru(bpy)3 2+ in the presence of either tripropylamine or sodium oxalate in pH 5 acetate buffer was also strongly dependent on the amount of Nafion introduced into the composite with greater ECL observed for the Nafion-silica films relative to pure Nafion.

  20. Electrogenerated chemiluminescence from R(bpy)3(2+) ion-exchanged in carbon nanotube/perfluorosulfonated ionomer composite films.

    Science.gov (United States)

    Guo, Zhihui; Dong, Shaojun

    2004-05-15

    The electrochemistry and electrogenerated chemiluminescence (ECL) of ruthenium(II) tris(bipyridine) (Ru(bpy)(3)(2+)) ion-exchanged in carbon nanotube (CNT)/Nafion composite films were investigated with tripropylamine (TPA) as a coreactant at a glassy carbon (GC) electrode. The major goal of this work was to investigate and develop new materials and immobilization approaches for the fabrication of ECL-based sensors with improved sensitivity, reactivity, and long-term stability. Ru(bpy)(3)(2+) could be strongly incorporated into Nafion film, but the rate of charge transfer was relative slow and its stability was also problematic. The interfusion of CNT in Nafion resulted in a high peak current of Ru(bpy)(3)(2+) and high ECL intensity. The results indicated that the composite film had more open structures and a larger surface area allowing faster diffusion of Ru(bpy)(3)(2+) and that the CNT could adsorb Ru(bpy)(3)(2+) and also acted as conducting pathways to connect Ru(bpy)(3)(2+) sites to the electrode. In the present work, the sensitivity of the ECL system at the CNT/Nafion film-modified electrodes was more than 2 orders of magnitude higher than that observed at a silica/Nafion composite film-modified electrode and 3 orders of magnitude higher than that at pure Nafion films. The CNT/Nafion composite film-modified GC electrodes also exhibited long-term stability.

  1. HPLC Determination of Ascorbic Acid Using Luminol-Cu(Ⅱ) Chemiluminescence-Application to the Analysis of Juice Beverage

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The chemiluminescence (CL) of luminol-Cu(Ⅱ) was applied to HPLC determination of ascorbic acid, which was separated by a C18 reverse-phase column with a mobile phase of 0.25 mol/L HAc. The eluted ascorbic acid was mixed with 0.3 mmol/L luminol and 0.05 mol/L CuSO4. The light emission from the reaction of Cu(Ⅱ) oxidized ascorbic acid and luminol was detected by a modified luminometer. The detection limit was 3.6×10-6 mol/L for ascorbic acid at a S/N ratio of 3, and the linear calibration range was 2×10-4- 2×10-3 mol/L. The relative standard deviation for 5 replicate injections of 1×10-3 mol/L ascorbic acid was calculated as 4.3 %. The method was successfully applied to determination of ascorbic acid in juice beverage.

  2. Silver nanoparticles enhanced a novel TCPO-H₂O₂-safranin O chemiluminescence system for determination of 6-mercaptopurine.

    Science.gov (United States)

    Biparva, Pourya; Abedirad, Seyed Mohammad; Kazemi, Sayed Yahya

    2015-06-15

    The present study deals with first attempt to introduce safranin O as the fluorophore for peroxyoxalate chemiluminescence system. The reaction of bis-(2,4,6-trichlorophenyl) oxalate (TCPO) with H2O2 catalyzed by silver nanoparticles can transfer energy to safranin O via the formation of dioxetanedione intermediate and emits orange-red light. The relationship between CL intensity and the concentration of TCPO, fluorophore, hydrogen peroxide and nanocatalyst was investigated. The Ag nanoparticles were synthesized by chemical reduction method and characterized using scanning electron microscopy, particle size analyzer and UV-spectroscopy. Moreover, the system was applied successfully to detect a drug, 6-mercaptopurine (6-MP) in pharmaceuticals. Under optimum conditions, a linear working range for 6-MP concentrations from 5.5 × 10(-7) to 5.5 × 10(-5)mol L(-1) (r>0.9831, n=6) was obtained with a detection limit of 1.6 × 10(-7)mol L(-1). The relative standard deviation for 6 repetitive determinations was less than 3.8% and recoveries of 98% and 103% were obtained.

  3. Molecularly imprinted polymer grafted paper-based multi-disk micro-disk plate for chemiluminescence detection of pesticide.

    Science.gov (United States)

    Wang, Shoumei; Ge, Lei; Li, Long; Yan, Mei; Ge, Shenguang; Yu, Jinghua

    2013-12-15

    The detection of pesticides has attracted considerable attention in numerous fields, such as environmental monitoring and food safety. Although traditional sensors for pesticides have been widely explored due to their high sensitivity and specificity, it is still challenging to develop a low-cost, portable, fast, and easy-to-use detection method for the public use at home or in the field. To address these challenges, herein, we report a novel paper-based molecular imprinted polymer (MIP)-grafted multi-disk micro-disk plate (P-MIP-MMP) for sensitive and specific chemiluminescence (CL) detection of pesticides through an indirect competitive assay using 2,4-dichlorophenoxyacetic acid (2,4-D) as a proof-of-concept analyte. The MIP-grafted paper disks were prepared by a simple in situ polymerization of MIP layer on the surface of cellulose fibers in paper. The quantification mechanism of this P-MIP-MMP is based on a competition between free 2,4-D and tobacco peroxidase (TOP) labeled 2,4-D and the enzyme catalyzed CL emission from the luminol-TOP-H2O2 CL system. At optimal conditions, this P-MIP-MMP can detect 2,4-D at the concentration of femtomolar level. This approach provided a powerful protocol for simple, low-cost, rapid, and high-throughput detection of pesticides in real samples with satisfactory results for use in areas such as food inspection and environmental monitoring.

  4. Solid-phase receptor-based assay for the detection of cyclic imines by chemiluminescence, fluorescence, or colorimetry.

    Science.gov (United States)

    Rodríguez, Laura P; Vilariño, Natalia; Molgó, Jordi; Aráoz, Rómulo; Antelo, Alvaro; Vieytes, Mercedes R; Botana, Luis M

    2011-08-01

    The spirolides and gymnodimines are marine phycotoxins included in the group of cyclic imines. The toxicity of these compounds to humans is still unknown, although their toxicity by intraperitoneal injection in rodents is very high. A receptor-based method was developed using the competition of the 13-desmethyl spirolide C with biotin-labeled α-bungarotoxin for binding to nicotinic acetylcholine receptors and the immobilization of the α-bungarotoxin-receptor complex on streptavidin-coated surfaces. The quantification of the immobilized receptor can be achieved using a specific antibody. Finally, after the addition of a secondary antibody labeled with horseradish peroxidase, three alternative substrates of this enzyme generate a chemiluminescent, fluorescent, or colorimetric signal. The assay performs well in shellfish extracts and the detection range is 5-150 nM of 13-desmethyl spirolide C in shellfish extracts, which is at least 5 times more sensitive than the existing fluorescence polarization assay. This assay can also detect gymnodimine, although with 10 times lower sensitivity than the spirolide. The detection of cyclic imines with microplate assays would be useful for screening purposes in order to reduce the number of samples to be processed by bioassays or analytical methods.

  5. A novel strategy for synthesis of hollow gold nanosphere and its application in electrogenerated chemiluminescence glucose biosensor.

    Science.gov (United States)

    Zhong, Xia; Chai, Ya-Qin; Yuan, Ruo

    2014-10-01

    Well-distributed hollow gold nanospheres (Aushell@GOD) (20 ± 5 nm) were synthesized using the glucose oxidase (GOD) cross-linked with glutaraldehyde as a template. A glucose biosensor was prepared based on Aushell@GOD nanospheres for catalyzing luminol electrogenerated chemiluminescence (ECL). Firstly, chitosan was modified in a glassy carbon electrode which offered an interface of abundant amino-groups to assemble Aushell@GOD nanospheres. Then, glucose oxidase was adsorbed on the surface of Aushell@GOD nanospheres via binding interactions between Aushell and amino groups of GOD to construct a glucose biosensor. The Aushell@GOD nanospheres were investigated with TEM and UV-vis. The ECL behaviors of the biosensor were also investigated. Results showed that, the obtained Aushell@GOD nanospheres exhibited excellent catalytic effect towards the ECL of luminol-H2O2 system. The response of the prepared biosensor to glucose was linear with the glucose concentration in the range of 1.0 μM to 4.3mM (R=0.9923) with a detection limit of 0.3 μM (signal to noise=3). This ECL biosensor exhibited short response time and excellent stability for glucose. At the same time the prepared ECL biosensor showed good reproducibility, sensitivity and selectivity.

  6. Determination of organic peroxides by liquid chromatography with on-line post-column ultraviolet irradiation and peroxyoxalate chemiluminescence detection.

    Science.gov (United States)

    Wada, Mitsuhiro; Inoue, Keiyu; Thara, Ayuko; Kishikawa, Naoya; Nakashima, Kenichiro; Kuroda, Naotaka

    2003-02-14

    A HPLC method was developed for the simultaneous determination of organic peroxides and hydrogen peroxide with peroxyoxalate chemiluminescence (PO-CL) detection following on-line UV irradiation. Organic peroxides [i.e., benzoyl peroxide (BP), tert.-butyl hydroperoxide (BHP), tert.-butyl perbenzoate (BPB), cumene hydroperoxide (CHP)] were UV irradiated (254 nm, 15 W) to generate hydrogen peroxide, which was determined by PO-CL detection. The conditions for UV irradiation and PO-CL detection were optimized by a flow injection analysis (FIA) system. Generation of hydrogen peroxide from peroxides with on-line UV irradiation also was confirmed by the FIA system by incorporating an enzyme column reactor immobilized with catalase. The separation of four organic peroxides and hydrogen peroxide by HPLC was accomplished isocratically on an ODS column within 30 min. The detection limits (signal-to-noise ratio=3) were 1.1 microM for hydrogen peroxide, 6.8 microM for BP, 31.3 microM for BHP, 7.5 microM for BPB and 1.3 microM for CHP. The proposed method was applied to the determination of BP in wheat flour.

  7. High-speed fuel tracer fluorescence and OH radical chemiluminescence imaging in a spark-ignition direct-injection engine.

    Science.gov (United States)

    Smith, James D; Sick, Volker

    2005-11-01

    An innovative technique has been demonstrated to achieve crank-angle-resolved planar laser-induced fluorescence (PLIF) of fuel followed by OH* chemiluminescence imaging in a firing direct-injected spark-ignition engine. This study used two standard KrF excimer lasers to excite toluene for tracking fuel distribution. The intensified camera system was operated at single crank-angle resolution at 2000 revolutions per minute (RPM) for 500 consecutive cycles. Through this work, it has been demonstrated that toluene and OH* can be imaged through the same optical setup while similar signal levels are obtained from both species, even at these high rates. The technique is useful for studying correlations between fuel distribution and subsequent ignition and flame propagation without the limitations of phase-averaging imaging approaches. This technique is illustrated for the effect of exhaust gas recirculation on combustion and will be useful for studies of misfire causes. Finally, a few general observations are presented as to the effect of preignition fuel distribution on subsequent combustion.

  8. Flow—injection Chemiluminescence Determination of Reserpine in Medicine and Biological Fluids with Controlled—Reagent—Release Technology

    Institute of Scientific and Technical Information of China (English)

    宋正华; 张尼

    2003-01-01

    A sensitive and rapid chemiluminescence(CL) flow injection with controlled-reagent-release technology for the determination of reserpine was proposed.The Cl reagents,luminol and dichromate,uses in this sensor,were all immobilized on anion-exchange resin.Through injection of 100μl of water,the reagents on the anion-ex-change resin column were eluted and in the presence of reserpine ,the CL intensity was decreased,by which reserpine could be sensed.Reserpine was quantified by measuring the decrement of CL intensity,which was observed linear with the logrithm of reserpine concentration in the rage of 1.0-500.0ng/mL,and the limit of detection was 0.4ng/mL(3σ)with a relative standard deviation of less than 3.0?The proposed procedure was applied in the assay of reserpine in pharmaceutical preparation and biological fhuids without any pre-treatment process and with sampling frequencies of 72 times per hour.

  9. Chemiluminescent Detection for Estimating Relative Copy Numbers of Porcine Endogenous Retrovirus Proviruses from Chinese Minipigs Based on Magnetic Nanoparticles.

    Science.gov (United States)

    Yang, Haowen; Liu, Ming; Zhou, Bingcong; Deng, Yan; He, Nongyue; Jiang, Hesheng; Guo, Yafen; Lan, Ganqiu; Jiang, Qinyang; Yang, Xiurong; Li, Zhiyang

    2016-06-01

    Chinese Bama minipigs could be potential donors for the supply of xenografts because they are genetically stable, highly inbred, and inexpensive. However, porcine endogenous retrovirus (PERV) is commonly integrated in pig genomes and could cause a cross-species infection by xenotransplantation. For screening out the pigs with low copy numbers of PERV proviruses, we have developed a novel semiquantitative analysis approach based on magnetic nanoparticles (MNPs) and chemiluminescence (CL) for estimating relative copy numbers (RCNs) of PERV proviruses in Chinese Bama minipigs. The CL intensities of PERV proviruses and the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were respectively determined with this method, and the RCNs of PERV proviruses were calculated by the equation: RCN of PERV provirus = CL intensity of PERV provirus/CL intensity of GAPDH. The results showed that PERVs were integrated in the genomes of Bama minipigs at different copy numbers, and the copy numbers of PERV-C subtype were greatly low. Two Bama minipigs with low copy numbers of PERV proviruses were detected out and could be considered as xenograft donor candidates. Although only semiquantitation can be achieved, this approach has potential for screening out safe and suitable pig donors for xenotransplantation.

  10. Flow injection chemiluminescence immunoassay based on resin beads, enzymatic amplification and a novel monoclonal antibody for determination of Hg(2+).

    Science.gov (United States)

    Xu, Mingxia; Chen, Mengting; Dong, Tiantian; Zhao, Kang; Deng, Anping; Li, Jianguo

    2015-09-21

    In the present work, a simple and sensitive flow injection chemiluminescent competitive immunoassay was developed for the determination of mercury(II) ion (Hg(2+)) based on carboxylic resin beads, a novel specific monoclonal antibody (McAb) and HRP enzyme-amplification. Resin beads with carboxyl groups were creatively employed as supports for immobilizing more coating antigen through acylamide bonds. With a competitive-type assay mode, the Hg(2+) in solution competed with the immobilized coating antigen for the limited McAb. Then, the second antibody labeled with HRP was introduced, and an effectively increased CL was obtained, which was ascribed to the catalytic activity of HRP for the luminol-PIP-H2O2 reaction. With increasing concentration of Hg(2+), the CL of this system decreases because less HRP is present in the CL reaction. At optimal conditions, the CL signal displayed a good linear relation toward Hg(2+) in the range of 0.05-200 ng mL(-1) with a detection limit (3σ) of 0.015 ng mL(-1). The immunosensor possessed high specificity, acceptable accuracy and reproducibility, and was examined in real samples with favorable results. This immunoassay will have intriguing application prospects for the determination of other heavy metal ions and environmental contaminants.

  11. Electrogenerated chemiluminescence of magnesium chlorophyllin a aqueous solution and its sensitive response to the carcinogen aflatoxin B1.

    Science.gov (United States)

    Li, Xiuting; Yuan, Hongyan; Li, Lan; Xiao, Dan

    2014-05-15

    The chlorophylls, crucial participants of photosynthesis, are large conjugated molecules with special electron donor-acceptor properties. Based on this, many investigations on the electrogenerated chemiluminescence (ECL) of chlorophyll a (Chl a) were performed in organic solvents, but no efficient signals were detected. Herein, ECL research of magnesium chlorophyllins a (Chlorins a) from simple saponification of the natural Chl a was carried out, and highly efficient and stable ECL signal was obtained for the first time. The mechanism study indicated that the ECL resulted from radical ion annihilation. Under the optimal conditions, the effect of the key gas O2 on the ECL of Chlorins a aqueous solution was investigated, and the recoverable inhibition of O2 was obviously observed. What is more, owing to the strong non-covalent interaction between Chlorins a and the carcinogen aflatoxin B1 (AFB1), ECL intensity of Chlorins a aqueous solution exhibited fast, sensitive and selective response to AFB1 with a low detection limit of 0.027 ppb at the signal-to-noise ratio of 3. The costless and environmentally friendly ECL method opens a new potential way of the rapid detection of AFB1 in practical application.

  12. Highly Chemiluminescent Graphene Oxide Hybrids Bifunctionalized by N-(Aminobutyl)-N-(Ethylisoluminol)/Horseradish Peroxidase and Sensitive Sensing of Hydrogen Peroxide.

    Science.gov (United States)

    Liu, Xiaoying; Han, Zhili; Li, Fang; Gao, Lingfeng; Liang, Gaolin; Cui, Hua

    2015-08-26

    N-aminobutyl-N-ethylisoluminol and horseradish peroxidase bifunctionalized graphene oxide hybrids (ABEI-GO@HRP) were prepared through a facile and green strategy for the first time. The hybrids exhibited excellent chemiluminescence (CL) activity over a wide range of pH from 6.1 to 13.0 when reacted with H2O2, whereas ABEI functionalized GO had no CL emission at neutral pH and showed more than 2 orders of magnitude lower CL intensity than ABEI-GO@HRP at pH 13.0. Such strong CL emission from ABEI-GO@HRP was probably due to that HRP and GO facilitated the formation of O2(•-), - CO4(•2-), HO(•), and π-C═C(•) in the CL reaction, and GO as a reaction interface promoted the electron transfer of the radical-involved reaction. By virtue of ABEI-GO@HRP as a platform, an ultrasensitive, selective, and reagentless CL sensor was developed for H2O2 detection. The CL sensor exhibited a detection limit of 47 fM at physiological pH, which was more than 2 orders of magnitude lower than previously reported methods. This work reveals that bifunctionalization of GO by ABEI and HRP leads to excellent CL feature and enzyme selectivity, which can be used as an ideal platform for developing novel analytical methods.

  13. Clinical Evaluation of the ZstatFlu-II Test: a Chemiluminescent Rapid Diagnostic Test for Influenza Virus

    Science.gov (United States)

    Hamilton, Marilyn S.; Abel, David M.; Ballam, Yolanda J.; Otto, Mary K.; Nickell, Angela F.; Pence, Lisa M.; Appleman, James R.; Shimasaki, Craig D.; Achyuthan, Komandoor E.

    2002-01-01

    Exploiting the high sensitivity of the chemiluminescence phenomenon, an accurate and sensitive point-of-care test, called the ZstatFlu-II test (ZymeTx, Inc., Oklahoma City, Okla.), was developed to detect influenza virus infections. The ZstatFlu-II test takes 20 min and requires approximately 2 min of “hands-on” time for operational steps. The ZstatFlu-II test does not distinguish between infections with influenza virus types A and B. ZstatFlu-II test results are printed on Polaroid High-Speed Detector Film, allowing test results to be archived. A prototype version of the ZstatFlu-II test was evaluated during the 2000-to-2001 flu season with 300 nasal aspirate specimens from children at a pediatric hospital. Compared to culture, the ZstatFlu-II test had 88% sensitivity and 92% specificity. The Directigen test had a sensitivity of 75% and a specificity of 93%. The sensitivity of the ZstatFlu-II test was significantly higher than that of the Directigen test (P < 0.0574). PMID:12089243

  14. A molecularly imprinted polymer based a lab-on-paper chemiluminescence device for the detection of dichlorvos

    Science.gov (United States)

    Liu, Wei; Guo, Yumei; Luo, Jing; Kou, Juan; Zheng, Hongyan; Li, Baoxin; Zhang, Zhujun

    2015-04-01

    In this work, a new molecularly imprinted polymer (MIP) based lab-on-paper device with chemiluminescence (CL) detection of dichlorvos (DDV) was designed. With the circle-shaped device, the MIP layer with certain depth was synthesized and adsorbed on the paper surface and DDV can be selectively imprinted on it. The adsorption and washing procedures can be achieved well on the paper-based chip. The paper-based device was fabricated by a simple cutting method and many chips can be made at the same time. On the basis of DDV enhancing CL of luminol-H2O2 greatly, the proposed MIP based lab-on-paper CL device showed better selectivity to DDV and it has been applied to the determination of DDV in vegetables in the range of 3.0 ng/mL-1.0 μg/mL with the detection limit of 0.8 ng/mL. This study has made a successful attempt in the development of highly selective and sensitive monitoring of DDV in real samples and will provide a new approach for sensitive and specific assay in environmental monitoring.

  15. Simultaneous detection of forbidden chemical residues in milk using dual-label time-resolved reverse competitive chemiluminescent immunoassay based on amine group functionalized surface.

    Directory of Open Access Journals (Sweden)

    Dongdong Zhang

    Full Text Available In this study, a sensitive dual-label time-resolved reverse competitive chemiluminescent immunoassay was developed for simultaneous detection of chloramphenicol (CAP and clenbuterol (CLE in milk. The strategy was performed based on the distinction of the kinetic characteristics of horseradish peroxidase (HRP and alkaline phosphatase (ALP in chemiluminesecence (CL systems and different orders of magnitude in HRP CL value for CAP and ALP CL value for CLE in the chemiluminescent immunoassay. Capture antibodies were covalently bound to the amine group functionalized chemiluminescent microtiter plate (MTP for efficient binding of detection antibodies for the enzymes labeled CAP (HRP-CAP and CLE (ALP-CLE. The CL signals were recorded at different time points by the automatic luminometers with significant distinction in the dynamic curves. When we considered the ALP CL value (about 10(5 of CLE as background for HRP CL signal value (about 10(7 of CAP, there was no interaction from ALP CL background of CLE and the differentiation of CAP and CLE can be easily achieved. The 50% inhibition concentration (IC50 values of CAP and CLE in milk samples were 0.00501 µg L(-1 and 0.0128 µg L(-1, with the ranges from 0.0003 µg L(-1 to 0.0912 µg L(-1 and from 0.00385 µg L(-1 to 0.125 µg L(-1, respectively. The developed method is more sensitive and of less duration than the commercial ELISA kits, suitable for simultaneous screening of CAP and CLE.

  16. Metabolism of benzoquinone by yeast cells and oxidative characteristics of corresponding hydroquinone: application to highly sensitive measurement of yeast cell density by using benzoquinone and a chemiluminescent probe.

    Science.gov (United States)

    Tsukatani, Tadayuki; Ide, Seiji; Ukeda, Hiroyuki; Matsumoto, Kiyoshi

    2004-07-01

    The metabolic efficiency of seven derivatives of 1,4-benzoquinone (BQ) by yeast cells and the oxidative characteristics of the corresponding hydroquinones (HQs) were studied by electrochemical, spectrophotometric and chemiluminescent methods. The spectrophotometric method was based on the reduction of a tetrazolium salt to formazan dye during the autoxidation of HQs generated by yeast cells under alkaline conditions. The amounts of HQs detected directly by the electrochemical method did not agree with those calculated from the formazan dye obtained by the spectrophotometric method. A tetrazolium salt was reduced to a formazan dye by both the superoxide anion radical (O2-*) generated during the autoxidation of 2,3,5,6-tetramethyl-1,4-HQ and by HQ itself. Little formazan dye was formed, and hydrogen peroxide (H2O2) was then finally produced during the autoxidation of 1,4-HQ or 2-methyl-1,4-HQ. Formazan dye and H2O2 were generated at a certain ratio during the autoxidation of derivatives of dimethyl-1,4-HQ or 2,3,5-trimethyl-1,4-HQ. The analytical method based on chemiluminescence with lucigenin and 2,3,5,6-tetramethyl-1,4-BQ was applied to highly sensitive measurement of the yeast cell density. A linear relationship between the chemiluminescence intensity and viable cell density was obtained in the range of 1.2 x 10(3) - 4.8 x 10(4) cells/ml. The detection limit was 4.8 x 10(2) cells/ml.

  17. Investigation on enhanced chemiluminescence reaction systems with bis(hydrogenperiodato) argentate(III) complex anion for fluoroquinolones synthetic antibiotics.

    Science.gov (United States)

    Sun, Hanwen; Chen, Peiyun; Wang, Fei; Wen, Haifang

    2009-07-15

    A novel chemiluminescence (CL) reaction system with bis(hydrogenperiodato) argentate(III) complex anion (Ag(III) complex, [Ag(HIO(6))(2)](5-)), for the first time, is developed for the determination of lomefloxacin (LMFX), enrofloxacin (ENLX) and pefloxacin (PFLX). The possible CL emission mechanism was discussed by comparing the fluorescence emission with CL spectra. The CL conditions of [Ag(HIO(6))(2)](5-)-H(2)SO(4)-LMFX/ENLX/PFLX systems were investigated and optimized. Under the optimized experimental conditions, the CL intensity is proportional to the concentration of the drugs in the range 0.2994-36.80x10(-7)g mL(-1) for LMFX, 4.00-30.0x10(-7)g mL(-1) for ENLX and 1.54-27.64x10(-7)g mL(-1) for PFLX. The limit of detection (s/n=3) was 9.1x10(-9)g mL(-1) for LMFX, 3.1x10(-9)g mL(-1) for ENLX and 4.4x10(-9)g mL(-1) for PFLX. The recovery of LMFX, ENLX and PELX from the spiked pharmaceutical preparations was in the range of 92.3-105% with the RSDs of 0.5-2.7%. For urine, serum and milk samples the recoveries of the three drugs were in the range of 85.1-107% for LMFX with the RSDs of 2.3-3.4%. 80.2-112% for ENLX with the RSDs of 1.4-2.8%, and 87.8-114% for PFLX with the RSDs of 1.6-2.7%. The proposed method was applied successfully to the determination of these compounds in real samples.

  18. A new chemiluminescence method for determination of dicyandiamide based on the N-bromosuccinimide–merbromin–cetyltrimethylammonium bromide system

    Energy Technology Data Exchange (ETDEWEB)

    Yu, Hua; Tang, Yuhai, E-mail: tyh57@mail.xjtu.edu.cn; Zhang, Guangbin; Wang, Zhongcheng; Gao, Ruixia

    2015-01-15

    A novel and highly sensitive chemiluminescence (CL) method for the determination of dicyandiamide (DCD) has been developed based on the new CL system of N-bromosuccinimide–merbromin–cetyltrimethylammonium bromide in alkaline solution. Experiment conditions were optimized using central composite design-response surface methodology. Under the optimal conditions, the relative CL intensity was linear with the concentration of DCD ranging from 5.0×10{sup −8} g mL{sup −1} to 3.0×10{sup −6} g mL{sup −1}. The detection limit, at the signal-to-noise ratio of 3, was 3.0×10{sup −9} g mL{sup −1}. The relative standard deviation was 1.9% for 11 repeated determinations of 1.0×10{sup −6} g mL{sup −1} DCD. The proposed method was successfully applied to the analyses of DCD in tap water and milk products. And the recoveries were in the range of 87.0–102.3% with relative standard deviation values of 1.2–2.9%. Moreover, the minimum sampling rate was 120 samples h{sup −1}. The possible mechanism of the CL reaction was also discussed. - Highlights: • A novel NBS–merbromin–CTAB–dicyandiamide CL system was proposed. • A CL method for detection of dicyandiamide was developed. • Chemical parameters were optimized using central composite design. • The proposed method is simple, rapid, sensitive, and low-cost.

  19. Antioxidant activity of Calendula officinalis extract: inhibitory effects on chemiluminescence of human neutrophil bursts and electron paramagnetic resonance spectroscopy.

    Science.gov (United States)

    Braga, Pier Carlo; Dal Sasso, Monica; Culici, Maria; Spallino, Alessandra; Falchi, Mario; Bertelli, Aldo; Morelli, Roberto; Lo Scalzo, Roberto

    2009-01-01

    There is growing interest in natural chemical compounds from aromatic, spicy, medicinal and other plants with antioxidant properties in order to find new sources of compounds inactivating free radicals generated by metabolic pathways within body tissue and cells, mainly polymorphonuclear leukocytes (PMNs) whose overregulated recruitment and activation generate a large amount of reactive oxygen species (ROS) and reactive nitrogen species (RNS), leading to an imbalance of redox homeostasis and oxidative stress. The aim of this study was to examine whether a propylene glycol extract of Calendula officinalis interferes with ROS and RNS during the PMN respiratory bursts, and to establish the lowest concentration at which it still exerts antioxidant activity by means of luminol-amplified chemiluminescence. Electron paramagnetic resonance (EPR) spectroscopy was also used in order to confirm the activity of the C. officinalis extract. The C. officinalis extract exerted its anti-ROS and anti-RNS activity in a concentration-dependent manner, with significant effects being observed at even very low concentrations: 0.20 microg/ml without L-arginine, 0.10 microg/ml when L-arginine was added to the test with phorbol 12-myristate 13-acetate and 0.05 microg/ml when it was added to the test with N-formyl-methionyl-leucyl-phenylalanine. The EPR study confirmed these findings, 0.20 microg/ml being the lowest concentration of C. officinalis extract that significantly reduced 2,2-diphenyl-1-picrylhydrazyl. These findings are interesting for improving the antioxidant network and restoring the redox balance in human cells with plant-derived molecules as well as extending the possibility of antagonizing the oxidative stress generated in living organisms when the balance is in favor of free radicals as a result of the depletion of cell antioxidants.

  20. Simple field device for measurement of dimethyl sulfide and dimethylsulfoniopropionate in natural waters, based on vapor generation and chemiluminescence detection.

    Science.gov (United States)

    Nagahata, Takanori; Kajiwara, Hidetaka; Ohira, Shin-Ichi; Toda, Kei

    2013-05-07

    A small, simple device was developed for trace analysis of dimethyl sulfide (DMS) and dimethylsulfoniopropionate (DMSP) in natural waters. These compounds are known to be the major sources of cloud condensation nuclei in the oceanic atmosphere and ideally should be measured onsite because of their volatility and instability. First, chemical and physical vapor generations were examined, and simple pressurizing by injection of 30 mL of air using a syringe was adopted. Pressurized headspace air above a 10 mL water sample was introduced to a detection cell as a result of the pressure differential and mixed with ozone to induce chemiluminescence. Although the measurement procedure was simple, the method was very sensitive: sharp peaks appeared within seconds for nanomolar levels of DMS, and the limit of detection was 0.02 nmol L(-1) (1 ng L(-1)). Although interference from methanethiol was significant, this was successfully addressed by adding a small amount of Cd(2+) before DMS vapor generation. DMSP was also measured after hydrolysis to DMS, as previously reported. Pond water and seawater samples were analyzed, and DMS was found in both types of sample, whereas DMSP was observed only in seawater. The DMS/DMSP data obtained using the developed method were compared with data obtained by purge/trap and gas chromatography-mass spectrometry, and the data from the two methods agreed, with good correlation (R(2) = 0.9956). The developed device is inexpensive, light (5 kg), simple to use, can be applied in the field, and is sensitive enough for fresh- and seawater analysis.

  1. Aptamer-barcode based immunoassay for the instantaneous derivatization chemiluminescence detection of IgE coupled to magnetic beads.

    Science.gov (United States)

    Peng, Qianwen; Cao, Zhijuan; Lau, Choiwan; Kai, Masaaki; Lu, Jianzhong

    2011-01-07

    We report on a highly sensitive aptameric assay system for the determination of IgE, where a special chemiluminescence (CL) reagent, 3,4,5-trimethoxylphenylglyoxal (TMPG), acts as the signaling molecule and polystyrene beads as the amplification platform. Briefly, a "sandwich-type" detection strategy is employed in our design, where magnetic beads functionalized with a capture antibody were reacted with the target protein IgE, and then sandwiched with the aptamer-barcodes which were prepared by assembling polystyrene beads with IgE aptamer. The target immunoreaction event could be sensitively detected via an instantaneous derivatization reaction between TMPG and the guanine (G) nucleotides within the aptamer-barcodes to form an unstable CL intermediate for the generation of light. Further signal amplification is achieved by extending the G nucleotide-rich domain on the aptamer backbone for second amplification. Such simple amplified CL transduction allows the detection of IgE down to the 4.6 pM level, which is better than most previous aptameric methods for IgE detection. This new protocol also provides a good capability in discriminating IgE from nontarget proteins such as IgG, IgA, IgM, interferon and thrombin. The practical application of the proposed aptamer-barcode based immunoassay was successfully carried out for the determination of IgE in 20 human serum samples. It is straightforward to adapt this strategy to detect a spectrum of other proteins by using different aptamers, thus this method may offer a new direction in designing high-performance CL aptasensors for early diagnoses of diseases.

  2. Flow injection analysis of organic peroxide explosives using acid degradation and chemiluminescent detection of released hydrogen peroxide.

    Science.gov (United States)

    Mahbub, Parvez; Zakaria, Philip; Guijt, Rosanne; Macka, Mirek; Dicinoski, Greg; Breadmore, Michael; Nesterenko, Pavel N

    2015-10-01

    The applicability of acid degradation of organic peroxides into hydrogen peroxide in a pneumatically driven flow injection system with chemiluminescence reaction with luminol and Cu(2+) as a catalyst (FIA-CL) was investigated for the fast and sensitive detection of organic peroxide explosives (OPEs). The target OPEs included hexamethylene triperoxide diamine (HMTD), triacetone triperoxide (TATP) and methylethyl ketone peroxide (MEKP). Under optimised conditions maximum degradations of 70% and 54% for TATP and HMTD, respectively were achieved at 162 µL min(-1), and 9% degradation for MEKP at 180 µL min(-1). Flow rates were precisely controlled in this single source pneumatic pressure driven multi-channel FIA system by model experiments on mixing of easily detectable component solutions. The linear range for detection of TATP, HMTD and H2O2 was 1-200 µM (r(2)=0.98-0.99) at both flow rates, while that for MEKP was 20-200 µM (r(2)=0.97) at 180 µL min(-1). The detection limits (LODs) obtained were 0.5 µM for TATP, HMTD and H2O2 and 10 µM for MEKP. The detection times varied from 1.5 to 3 min in this FIA-CL system. Whilst the LOD for H2O2 was comparable with those reported by other investigators, the LODs and analysis times for TATP and HMTD were superior, and significantly, this is the first time the detection of MEKP has been reported by FIA-CL.

  3. A cascade amplification strategy based on rolling circle amplification and hydroxylamine amplified gold nanoparticles enables chemiluminescence detection of adenosine triphosphate.

    Science.gov (United States)

    Wang, Ping; Zhang, Tonghuan; Yang, Taoyi; Jin, Nan; Zhao, Yanjun; Fan, Aiping

    2014-08-07

    A highly sensitive and selective chemiluminescent (CL) biosensor for adenosine triphosphate (ATP) was developed by taking advantage of the ATP-dependent enzymatic reaction (ATP-DER), the powerful signal amplification capability of rolling circle amplification (RCA), and hydroxylamine-amplified gold nanoparticles (Au NPs). The strategy relies on the ability of ATP, a cofactor of T4 DNA ligase, to trigger the ligation-RCA reaction. In the presence of ATP, the T4 DNA ligase catalyzes the ligation reaction between the two ends of the padlock probe, producing a closed circular DNA template that initiates the RCA reaction with phi29 DNA polymerase and dNTP. Therein, many complementary copies of the circular template can be generated. The ATP-DER is eventually converted into a detectable CL signal after a series of processes, including gold probe hybridization, hydroxylamine amplification, and oxidative gold metal dissolution coupled with a simple and sensitive luminol CL reaction. The CL signal is directly proportional to the ATP level. The results showed that the detection limit of the assay is 100 pM of ATP, which compares favorably with those of other ATP detection techniques. In addition, by taking advantage of ATP-DER, the proposed CL sensing system exhibits extraordinary specificity towards ATP and could distinguish the target molecule ATP from its analogues. The proposed method provides a new and versatile platform for the design of novel DNA ligation reaction-based CL sensing systems for other cofactors. This novel ATP-DER based CL sensing system may find wide applications in clinical diagnosis as well as in environmental and biomedical fields.

  4. Application of a Newly Developed High-Sensitivity HBsAg Chemiluminescent Enzyme Immunoassay for Hepatitis B Patients with HBsAg Seroclearance

    OpenAIRE

    2013-01-01

    We modified and automated a highly sensitive chemiluminescent enzyme immunoassay (CLEIA) for surface antigen (HBsAg) detection using a combination of monoclonal antibodies, each for a specific epitope of HBsAg, and by improving an earlier conjugation technique. Of 471 hepatitis B virus (HBV) carriers seen in our hospital between 2009 and 2012, 26 were HBsAg seronegative as determined by the Abbott Architect assay. The Lumipulse HBsAg-HQ assay was used to recheck those 26 patients who demonstr...

  5. Electrogenerated chemiluminescence sensor for the determination of metoclopramide using ordered mesoporous carbon for immobilizing tris(2,2 -bipyridyl)ruthenium

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    A novel electrogenerated chemiluminescence(ECL)sensor for the determination of metoclopramide was developed by employing ruthenium complex as an ECL signal producer and an ordered mesoporous carbon(OMC)material as modified material.The ECL sensor was fabricated by adsorption ruthenium complex into a mixture of OMC and Nafion,which showed good electrochemical and ECL behaviors.It was found that the ECL intensity of the sensor fabricated was greatly enhanced in the presence of metoclopramide.Based on this fin...

  6. A sensitive and selective chemiluminescence sensor for the determination of dopamine based on silanized magnetic graphene oxide-molecularly imprinted polymer

    Science.gov (United States)

    Duan, Huimin; Li, Leilei; Wang, Xiaojiao; Wang, Yanhui; Li, Jianbo; Luo, Chuannan

    2015-03-01

    Based on silanized magnetic graphene oxide-molecularly imprinted polymer (Si-MG-MIP), a sensitive and selective chemiluminescence sensor for dopamine measurement was developed. Si-MG-MIP, in which silanes was introduced to improve the mass transfer, graphene oxide was employed to improve absorption capacity, Fe3O4 nanoparticles were applied for separation easily and molecularly imprinted polymer was used to improve selectivity, demonstrated the advantages of the sensor. All the composites were confirmed by SEM, TEM, XRD and FTIR. Under the optimal conditions of chemiluminescence, dopamine could be assayed in the range of 8.0-200.0 ng/mL with a correlation coefficient of linear regression of 0.9970. The detection limit was 1.5 ng/mL (3δ) and the precision for 11 replicate detections of 80.0 ng/mL dopamine was 3.4% (RSD). When the sensor was applied in determining dopamine in actual samples, recovery ranged from 94% to 110%, which revealed that the results were satisfactory.

  7. QM/MM study on the light emitters of aequorin chemiluminescence, bioluminescence, and fluorescence: a general understanding of the bioluminescence of several marine organisms.

    Science.gov (United States)

    Chen, Shu-Feng; Ferré, Nicolas; Liu, Ya-Jun

    2013-06-24

    Aequorea victoria is a type of jellyfish that is known by its famous protein, green fluorescent protein (GFP), which has been widely used as a probe in many fields. Aequorea has another important protein, aequorin, which is one of the members of the EF-hand calcium-binding protein family. Aequorin has been used for intracellular calcium measurements for three decades, but its bioluminescence mechanism remains largely unknown. One of the important reasons is the lack of clear and reliable knowledge about the light emitters, which are complex. Several neutral and anionic forms exist in chemiexcited, bioluminescent, and fluorescent states and are connected with the H-bond network of the binding cavity in the protein. We first theoretically investigated aequorin chemiluminescence, bioluminescence, and fluorescence in real proteins by performing hybrid quantum mechanics and molecular mechanics methods combined with a molecular dynamics method. For the first time, this study reported the origin and clear differences in the chemiluminescence, bioluminescence and fluorescence of aequorin, which is important for understanding the bioluminescence not only of jellyfish, but also of many other marine organisms (that have the same coelenterazine caved in different coelenterazine-type luciferases).

  8. Chemiluminescent determination of vanadium(IV) using a cinchomeronic hydrazide-H{sub 2}O{sub 2} system and flow injection analysis

    Energy Technology Data Exchange (ETDEWEB)

    Pradana Perez, J.A. [Departamento de Ciencias Analiticas, Facultad de Ciencias, Universidad Nacional de Educacion a Distancia, c/Senda del Rey 9, 28040 Madrid (Spain); Alegria, J.S. Durand [Departamento de Ciencias Analiticas, Facultad de Ciencias, Universidad Nacional de Educacion a Distancia, c/Senda del Rey 9, 28040 Madrid (Spain); Hernando, P. Fernandez [Departamento de Ciencias Analiticas, Facultad de Ciencias, Universidad Nacional de Educacion a Distancia, c/Senda del Rey 9, 28040 Madrid (Spain)]. E-mail: pfhernando@ccia.uned.es; Sierra, A. Narros [Departamento de Ingenieria Quimica y del Medio Ambiente, Escuela Tecnica Superior de Ingenieros Industriales, Universidad Politecnica de Madrid, Madrid (Spain)

    2005-04-22

    This paper proposes a new chemiluminescent flow injection analysis (FIA) method for the determination of vanadium(IV) ions in aqueous media. The method is based on the chemiluminescent reaction that occurs between cinchomeronic hydrazide (CH) and hydrogen peroxide in a strongly alkaline medium, in which vanadium(IV) acts as a catalyst. The chemical and physical variables involved in the flow injection system are optimised using a modified simplex method. Vanadium ions can be detected in the 0.08 and 1.00 {mu}g mL{sup -1} range; the detection limit for a signal-to-noise ratio of 3 is 0.08 {mu}g mL{sup -1}. Great variations in the quantum yield were observed when cobalt(II), chromium(III), copper(II) and/or nickel(II) were present in the reaction medium. The proposed method is selective and simple, and can be successfully used to analyse water samples without the need for separation or preconcentration processes.

  9. Detection of pyrrolizidine alkaloids using flow analysis with both acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence

    Energy Technology Data Exchange (ETDEWEB)

    Gorman, Bree A. [School of Biological and Chemical Sciences, Deakin University, Geelong, Vic. 3217 (Australia); Barnett, Neil W. [School of Biological and Chemical Sciences, Deakin University, Geelong, Vic. 3217 (Australia)]. E-mail: barnie@deakin.edu.au; Bos, Richard [School of Biological and Chemical Sciences, Deakin University, Geelong, Vic. 3217 (Australia)

    2005-06-13

    For the first time, analytically useful chemiluminescence was elicited from the reactions of the pyrrolizidine alkaloids. Heliotrine, retronecine, supinine, monocrotaline and echinatine N-oxide yielded chemiluminescence upon reaction with tris(2,2'-bipyridyl)ruthenium(II) whilst lasiocarpine, its N-oxide and supinine elicited light upon reaction with acidic potassium permanganate. Detection limits for heliotrine were 1.25 x 10{sup -7} M and 9 x 10{sup -9} M for tris(2,2'-bipyridyl)ruthenium(III) perchlorate with flow injection analysis (FIA) and the silica-immobilised reagent (4-[4-(dichloromethylsilanyl)-butyl]-4'-methyl-2,2'-bipyridine)bis (2,2'-bipyridyl)ruthenium(II) with sequential injection analysis (SIA), respectively. Lasiocarpine was detectable at 1.4 x 10{sup -7} M using acidic potassium permanganate with FIA. Additionally, the silica-immobilised reagent was optimised with respect to the oxidant (ammonium ceric nitrate) concentration and the aspiration times which afforded a detection limit for codeine of 5 x 10{sup -10} M using SIA.

  10. Highly sensitive trivalent copper chelate–luminol chemiluminescence system for capillary electrophoresis chiral separation and determination of ofloxacin enantiomers in urine samples

    Directory of Open Access Journals (Sweden)

    Hao-Yue Xie

    2014-12-01

    Full Text Available A simple, fast and sensitive capillary electrophoresis (CE strategy combined with chemiluminescence (CL detection for analysis of ofloxacin (OF enantiomers was established in the present work. Sulfonated β-cyclodextrin (β-CD was used as the chiral additive being added into the running buffer of luminol–diperiodatocuprate (III (K5[Cu(HIO62], DPC chemiluminescence system. Under the optimum conditions, the proposed method was successfully applied to separation and analysis of OF enantiomers with the detection limits (S/N=3 of 8.0 nM and 7.0 nM for levofloxacin and dextrofloxacin, respectively. The linear ranges were both 0.010–100 μM. The method was utilized for analyzing OF in urine; the results obtained were satisfactory and recoveries were 89.5–110.8%, which demonstrated the reliability of this method. This approach can also be further extended to analyze different commercial OF medicines.

  11. Chemiluminescence determination of moxifloxacin in pharmaceutical and biological samples based on its enhancing effect of the luminol-ferricyanide system using a microfluidic chip.

    Science.gov (United States)

    Suh, Yeoun Suk; Kamruzzaman, Mohammad; Alam, Al-Mahmnur; Lee, Sang Hak; Kim, Young Ho; Kim, Gyu-Man; Dang, Trung Dung

    2014-05-01

    A sensitive determination of a synthetic fluoroquinolone antibacterial agent, moxifloxacin (MOX), by an enhanced chemiluminescence (CL) method using a microfluidic chip is described. The microfluidic chip was fabricated by a soft-lithographic procedure using polydimethyl siloxane (PDMS). The fabricated PDMS microfluidic chip had three-inlet microchannels for introducing the sample, chemiluminescent reagent and oxidant, and a 500 µm wide, 250 µm deep and 82 mm long microchannel. An enhanced CL system, luminol-ferricyanide, was adopted to analyze the MOX concentration in a sample solution. CL light was emitted continuously after mixing luminol and ferricyanide in the presence of MOX on the PDMS microfluidic chip. The amount of MOX in the luminol-ferricyanide system influenced the intensity of the CL light. The linear range of MOX concentration was 0.14-55.0 ng/mL with a correlation coefficient of 0.9992. The limit of detection (LOD) and limit of quantification (LOQ) were 0.06 and 0.2 ng/mL respectively. The presented method afforded good reproducibility, with a relative standard deviation (RSD) of 1.05% for 10 ng/mL of MOX, and has been successfully applied for the determination of MOX in pharmaceutical and biological samples.

  12. Electrogenerated chemiluminescence quenching of Ru(bpy){sub 3} {sup 2+} (bpy=2,2 Prime -bipyridine) in the presence of acetaminophen, salicylic acid and their metabolites

    Energy Technology Data Exchange (ETDEWEB)

    Haslag, Catherine S. [Department of Chemistry, Missouri State University, Springfield, Missouri 65897 (United States); Richter, Mark M., E-mail: MarkRichter@missouristate.edu [Department of Chemistry, Missouri State University, Springfield, Missouri 65897 (United States)

    2012-03-15

    Quenching of Ru(bpy) {sub 3}{sup 2+} (bpy=2,2 Prime -bipyridine) coreactant electrogenerated chemiluminescence (ECL) has been observed in the presence of acetaminophen, salicylic acid and related complexes. However, no quenching is observed with the acetylsalicylic acid. In most instances, quenching is observed with 100-fold excess of quencher (compared to ECL luminophore) with complete quenching observed between 10,000 and 100,000 fold excess. Fluorescence and UV-vis experiments coupled with bulk electrolysis support the formation of benzoquinone products upon electrochemical oxidation. The mechanism of quenching may involve the interaction of the electrochemically generated benzoquinone species with (i) the {sup Low-Asterisk }Ru(bpy){sub 3}{sup 2+} excited state or (ii) highly energetic coreactant radicals. - Highlights: Black-Right-Pointing-Triangle Efficient quenching of the electrogenerated chemiluminescence is observed. Black-Right-Pointing-Triangle Acetaminophen, salicylic acid and related compounds can be detected. Black-Right-Pointing-Triangle The mechanism of quenching involves benzoquinones formed upon electrolysis.

  13. A Novel Electrogenerated Chemiluminescence Reaction Scheme Using Core-Shell Luminol-Based SiO2 Nanoparticles as a Regulator and Its Analytical Application

    Institute of Scientific and Technical Information of China (English)

    ZHANG Li-Li; ZHENG Xing-Wang; GUO Zhi-Hui

    2007-01-01

    A novel core-shell luminol-based SiO2 nanoparticle was synthesized by two step micro-emulsion method.While these nanoparticles were used as electrogenerated chemiluminescence (ECL) reagent, the electrochemical (EC) reaction as well as the subsequent chemiluminescence (CL) reaction not only could be separated spatially, but also presented high efficiency for analytical purpose. In this case, the core-shell luminol-based SiO2 nanoparticles offered more potential to avoid the contradiction between the EC and the CL reaction conditions. A new ECL method based on the nanoparticle was developed, and isoniazid was selected as a model analyte to illustrate the characteristics of this new ECL method. Under the selected conditions, the proposed ECL response to isoniazid concentration was linear in the range of 1.0 × 10-10 to 1.0 × 10-6 g/mL with 2 × 10-11 g/mL detection limit.

  14. Chemiluminescence system for direct determination and mapping of ultra-trace metal impurities on a silicon wafer.

    Science.gov (United States)

    Kim, Romertta; Sung, Y I; Lee, J S; Lim, H B

    2010-11-01

    A highly sensitive chemiluminescence (CL) system which consumed low sample and reagent volumes in the microlitre range was developed for direct determination and mapping of ultra-trace metal contaminants on solid surfaces, such as silicon wafers or flat display panels. The analytical result of the system was confirmed with ICP-MS. The system was composed of a scanner, sensor and a wafer moving stage. The scanner, with a scanning tip made of 0.03'' i.d. PTFE tubing, was used to collect metal impurities on the wafer surface with 5 μL of scanning solution. A coaxial sensing head of about 13 mm o.d. and 110 mm height was designed both to inject a luminescent reagent of luminol-H(2)O(2) mixture and to collect the luminescence light resulting from the reaction with metal ions of Co(2+), Fe(2+), Cu(2+), and Ni(2+). Due to the almost zero background, an extremely low limit of detection of 20.8 pg/mL for Co(2+) in 1% hydrofluoric acid (HF) was obtained from the calibration curve. In order to map the spatial distribution of the impurities, 11 cross sections of a Co-contaminated wafer were selected and scanned individually with a diluted HF solution. A contaminant level of 1.45-7.11 × 10(11) atoms cm(-2) was obtained for each section with an average of 4.21 × 10(11) atoms cm(-2), which was similar to the analytical result of 5.48 × 10(11) atoms cm(-2) obtained from vapor phase deposition-inductively coupled plasma-mass spectrometry (VPD-ICP-MS). Although this CL system does not have selectivity for each specific metal ion, its high sensitivity facilitates the monitoring and mapping of metal impurities of Co, Fe, Cu, etc. on the wafer directly and it can be used as an on-line inspection sensor for the first time in the semiconductor industry.

  15. Comparative studies of the chemiluminescent horseradish peroxidase-catalyzed peroxidation of acridan (GZ-11) and luminol reactions: Effect of pH and scavengers of reactive oxygen species on the light intensity of these systems

    NARCIS (Netherlands)

    Osman, A.M.; Zomer, G.; Laane, C.; Hilhorst, R.

    2000-01-01

    In this study, the chemiluminescent horseradish peroxidase/H2O2-catalysed oxidation of acridan (GZ-11) substrate was compared with the well-characterized light-producing luminol reaction. p-Iodophenol and p-phenylphenol were used as enhancers, respectively, for the luminol and acridan reactions. The

  16. Ultrasensitive Infrared Chemiluminescence Detection

    Science.gov (United States)

    1993-10-01

    Phys. 21. 649 70:± 7 2.3 ± 0.6 6.9 ± 0.7 0.9 ± 0.2 Vincent -Geiss (1954) Compt. Read. 239, 231 82 8.1 BriMe (1958) J. Chem. Phys. 28. 11; 70 2.7 6.9 1.1...Cho 0.1, , 0 4 8 12 16 20 24 Upper Vibrfanal Level 47 GOGH ISE Values From p$r) Fit: Quadratic -Cubic 48 0 cc cm Oi Co Al 00 *amooN 0+0Z C4N cc go IV

  17. Flow-injection chemiluminescence determination of chlortetracycline using on-line electrogenerated [Cu(HIO(6))(2)](5-) as the oxidant.

    Science.gov (United States)

    Li, B; Zhang, Z; Liu, W

    2001-12-24

    A novel chemiluminescence (CL) flow system for the determination of chlortetracycline is described. It is based on the direct CL reaction of chlortetracycline and [Cu(HIO(6))(2)](5-) in KOH medium. The unstable [Cu(HIO(6))(2)](5-) was on-line electrogenerated by constant-current electrolysis. The CL intensity was linear with chlortetracycline concentration in the range of 0.1-100 mug ml(-1). The determination limit was 5.3x10(-8) g ml(-1). The whole process could be completed in 1 min. The proposed method is suitable for automatic and continuous analysis, and has been applied satisfactorily to analysis of chlortetracycline in biological fluid.

  18. Development of chemiluminescence method for determination of 10-hydroxycamptothecin based on luminol-[Ag(HIO₆)₂]⁵⁻ reaction in alkaline solution.

    Science.gov (United States)

    Sun, Hanwen; Chen, Peiyun; Shi, Shasha; Li, Liqing

    2011-01-01

    A novel chemiluminescence (CL) method was developed for the determination of 10-hydroxycamptothecin(HCPT) based on the CL reaction between [Ag(HIO₆)₂]⁵⁻ and luminol in alkaline solution. CL emission of Ag(III) complex-luminol in alkaline medium was very different from that in acidic medium. A possible mechanism of enhanced CL emission was suggested. The enhanced effect of HCPT on CL emission of the [Ag(HIO₆)₂]⁵⁻-luminol system was found. The enhanced degree of CL emission was proportional to HCPT concentration. The effect of the reaction conditions on CL emission was examined. Under optimal conditions, the limit of detection was 6.5 × 10⁻⁹ g mL⁻¹. The proposed method was applied for the determination of HCPT in real samples with the recoveries of 93.2-109% with the RSD of 1.7-3.3%.

  19. Co-metal-organic-frameworks with pure uniform crystal morphology prepared via Co2 + exchange-mediated transformation from Zn-metallogels for luminol catalysed chemiluminescence

    Science.gov (United States)

    Tang, Xue Qian; Xiao, Bo Wen; Li, Chun Mei; Wang, Dong Mei; Huang, Cheng Zhi; Li, Yuan Fang

    2017-03-01

    Cation exchange-mediated transformation from Zn-metallogels (MOGs), which was a mild facile strategy relative to the demanding hydrothermal method, was employed to develop Co2 + metal-organic frameworks (Co-MOFs) at room temperature. The obtained Co-MOFs was of uniform octahedral morphology and possessed high activity to catalyze luminol chemiluminescence without extra oxidants. By adding cysteine, the CL emission of luminol-Co-MOFs system was further enhanced. Based on this phenomenon, Co-MOFs was utilized to build a practical sensing platform for cysteine determination. Under the optimized conditions, the relative CL intensity (ΔI) was proportional to the concentration of cysteine in the range of 2-10 μM, and the detection limit was 0.49 μM (3S/N). Moreover, the established method was applied to the determination of cysteine in commercially available pharmaceutical injections.

  20. Desenvolvimento de um dispositivo de baixo custo para medidas por quimiluminescência Development of a low cost device for chemiluminescence measurements

    Directory of Open Access Journals (Sweden)

    Eduardo P. Borges

    2002-12-01

    Full Text Available A simple and low cost device (ca. US$ 150 that comprises two photodiodes fixed in lab-made Perspex flow cell is proposed for chemiluminescence measurements. The characteristics of the device (large observation window and reduced thickness allow maximizing the amount of the emitted radiation detected. A sensitivity improvement of ca. 50 % was observed by employing two photodiodes for signal measurements. The performance of the device was assessed by the oxidation of luminol by hydrogen peroxide, yielding a linear response within the range of 2.50 to 500 µmol L-1 H2O2. The detection limit was estimated as 0.8 µmol L-1 hydrogen peroxide which is comparable with those obtained by using equipments based on photomultipliers.

  1. A Chemiluminescence Optical Fiber Glucose Biosensor Based on Co-immobilizing Glucose Oxidase and Horseradish Peroxidase in a Sol-gel Film

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    An optical fiber bienzyme sensor based on the luminol chemiluminescent reaction was developed and demonstrated to be sensitive to glucose. Glucose oxidase(GOD) and horseradish peroxidase(HRP) were co-immobilized by microencapsulation in a sol-gel film derived from tetraethyl orthosilicate(TEOS). The calibration plots for glucose were established by the optical fiber glucose sensor fabricated by attaching the bienzyme silica gel onto the glass window of the fiber bundle. The linear range was 0.2-2 mmol/L and the detection limit was approximately 0.12 mmol/L. The relative standard deviation was 5.3% (n=6). The proposed biosensor was applied to glucose assay in ofloxacin injection successfully.

  2. Electrogenerated chemiluminescence of tris(2,2' bipyridine)ruthenium(II) using common biological buffers as co-reactant, pH buffer and supporting electrolyte.

    Science.gov (United States)

    Kebede, Noah; Francis, Paul S; Barbante, Gregory J; Hogan, Conor F

    2015-11-07

    A series of aliphatic tertiary amines (HEPES, POPSO, EPPS and BIS-TRIS) commonly used to buffer the pH in biological experiments, were examined as alternative, non-toxic co-reactants for the electrogenerated chemiluminescence (ECL) of tris(2,2'-bipyridine)ruthenium(ii) ([Ru(bpy)3](2+)). These were found to be very attractive as "multi-tasking" reagents, serving not only as co-reactants, but also fulfiling the roles of pH buffer and supporting electrolyte within an aqueous environment; thus significantly simplifying the overall ECL analysis. Sub-nanomolar detection limits were obtained for [Ru(bpy)3](2+) in the presence of BIS-TRIS, making this species an valuable option for co-reactant ECL-based bioanalytical applications.

  3. 化学发光法测定油品氮含量的研究%Study on Chemiluminescence Method for the Determination of Nitrogen Content of Oil

    Institute of Scientific and Technical Information of China (English)

    付晶丽

    2012-01-01

    Using chemiluminescence method for the determination of nitrogen content in oil products,mainly inspects the quality measurement of appropriate sample size in oil,nitrogen is converted to NO the best conditions and measurement precision,improve the correctness and practicability of the method.%用化学发光法测定油品中的氮含量,主要考察了用质量法测量比较合适的进样量、油品中氮转化为NO的最佳条件及测量的精密度,提高了该方法的准确性和实用性。

  4. Simultaneous monitoring of superoxides and intracellular calcium ions in neutrophils by chemiluminescence and fluorescence: evaluation of action mechanisms of bioactive compounds in foods.

    Science.gov (United States)

    Kazumura, Kimiko; Sato, Yukiko; Satozono, Hiroshi; Koike, Takashi; Tsuchiya, Hiroshi; Hiramatsu, Mitsuo; Katsumata, Masakazu; Okazaki, Shigetoshi

    2013-10-01

    We have developed a measuring system for simultaneous monitoring of chemiluminescence and fluorescence, which indicate respectively, (i) generation of superoxide anion radicals (O2(-•)) and (ii) change in the intracellular calcium ion concentration ([Ca(2+)]i) of neutrophils triggered by the mechanism of innate immune response. We applied this measuring system for establishing a method to distinguish between anti-inflammatory actions and antioxidant actions caused by bioactive compounds. We evaluated anti-inflammatory agents (zinc ion [Zn(2+)] and ibuprofen) and antioxidants (superoxide dismutase [SOD] and ascorbic acid). It was shown that ibuprofen and Zn(2+) were anti-inflammatory while SOD and ascorbic acid were anti-oxidative. We conclude that it is possible to determine the mechanism of action of bioactive compounds using this method.

  5. On the use of L-012, a luminol-based chemiluminescent probe, for detecting superoxide and identifying inhibitors of NADPH oxidase: a reevaluation.

    Science.gov (United States)

    Zielonka, Jacek; Lambeth, J David; Kalyanaraman, Balaraman

    2013-12-01

    L-012, a luminol-based chemiluminescent (CL) probe, is widely used in vitro and in vivo to detect NADPH oxidase (Nox)-derived superoxide (O2(*-)) and identify Nox inhibitors. Yet understanding of the free radical chemistry of the L-012 probe is still lacking. We report that peroxidase and H2O2 induce superoxide dismutase (SOD)-sensitive, L-012-derived CL in the presence of oxygen. O2(*-) alone does not react with L-012 to emit luminescence. Self-generated O2(*-) during oxidation of L-012 and luminol analogs artifactually induce CL inhibitable by SOD. These aspects make assays based on luminol analogs less than ideal for specific detection and identification of O2(*-) and NOX inhibitors.

  6. Chemiluminescence determination of antioxidant property of Zizyphus mistol and Prosopis alba during oxidative stress generated in blood by Hemolytic Uremic Syndrome-producing Escherichia coli.

    Science.gov (United States)

    Albrecht, Claudia; Pellarin, María G; Baronetti, José; Rojas, María J; Albesa, Inés; Eraso, Alberto J

    2011-01-01

    This study was undertaken to elucidate the antioxidant effect of Zizyphus mistol and Prosopis alba, with the hypothesis that these fruits can counteract the induction of reactive oxygen species (ROS) caused by toxins produced by Escherichia coli. In the search of nutrients effective against the Hemolytic Uremic Syndrome (HUS), we detected by chemiluminescence a protective role of both plants, due to their natural antioxidants significantly decreasing the levels of ROS induced by toxins from E. coli in blood. The ferric reducing antioxidant power (FRAP) was found to be higher in Z. mistol than in P. alba. The chemical analyses of the phenols and flavonoids present in the fruit extracts indicated that the FRAP correlated with the amount of phenolic compounds, but not with the flavonoids analyzed. Both fruits studied reduce the induction of ROS, and in this way help to prevent the development of complications related to oxidative stress generated in the blood of patients with HUS.

  7. Vibrational population distributions of the product of the chemiluminescent charge transfer reaction: O + (2D)+HCl --> O+HCl + (A 2Sigma + )

    Science.gov (United States)

    Shiraishi, Yasushi; Kusunoki, Isao

    1987-12-01

    The chemiluminescent charge transfer reaction of O+ (2 D)ions with HCl has been studied in the energy range of 7 to 100 eVc.m. . The vibrational population distributions of the HCl+(A 2 Σ+,v') product have been analyzed from the emission spectra of the A→X transition. At low collision energy the distribution has a peak at v'=3. The partial reaction cross section σ(3) for this level increases with decreasing collision energy. The features of the reaction are very similar to the F+ +CO→ F+CO+ (A) reaction reported previously. The mechanism has been discussed on the basis of curve crossing between the initial and final states along the HCl vibrational coordinate in the asymptotic region of the [O-HCl]+ system. The projectile ions C+ and N+ have also been tested for the charge transfer reaction of HCl(X)→HCl+(A).

  8. A sensitive inhibition chemiluminescence method for the determination of 6-mercaptopurine in tablet and biological fluid using the reaction of luminol-Ag(III) complex in alkaline medium

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Hanwen, E-mail: hanwen@hbu.edu.cn [College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002 (China); Wang, Ting; Liu, Xuyang; Chen, Peiyun [College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002 (China)

    2013-02-15

    A sensitive inhibition chemiluminescence (CL) method for the determination of 6-mercaptopurine (6-MP) is developed. The mechanism of the CL reaction between Ag(III) complex {l_brace}[Ag(HIO{sub 6}){sub 2}]{sup 5-}{r_brace} and luminol in alkaline solution was proposed, along with the inhibition mechanism of 6-MP on the CL emission. The inhibition degree of CL emission was proportional to the logarithm of 6-MP concentration. The effects of the reaction conditions on CL emission and inhibition were examined. Under the optimized conditions, the detection limit (s/n=3) was 3.7 Multiplication-Sign 10{sup -10} g ml{sup -1}. The recoveries of 6-MP were in the range of 97.7-105% with the RSD of 2.1-3.4% (n=5) for tablet samples, 103-106% with the RSDs of 1.1-2.1% for spiked serum sample, and 97.2-101% with the RSD of 2.0-4.5% for spiked urine sample. The accuracy of this method for the tablet analysis was examined by comparing with the pharmacopoeia method. The proposed method was used for the determination of 6-MP at clinically relevant concentrations in real urine and serum samples with satisfactory results. - Highlights: Black-Right-Pointing-Pointer A sensitive inhibition chemiluminescence (CL) method for the determination of 6-MP is developed. Black-Right-Pointing-Pointer The inhibition mechanism of 6-MP on the CL emission was proposed. Black-Right-Pointing-Pointer The detection limit was 3.7 Multiplication-Sign 10{sup -10} g ml{sup -1}. Black-Right-Pointing-Pointer The accuracy was examined by comparing with the pharmacopoeia method.

  9. An enhanced chemiluminescence resonance energy transfer system based on target recycling G-guadruplexes/hemin DNAzyme catalysis and its application in ultrasensitive detection of DNA.

    Science.gov (United States)

    Chen, Jia; Huang, Yong; Vdovenko, Marina; Sakharov, Ivan Yu; Su, Guifa; Zhao, Shulin

    2015-06-01

    An enhanced chemiluminescence resonance energy transfer (CRET) system based on target recycling G-guadruplexes/hemin DNAzyme catalysis was developed for ultrasensitive detection of DNA. CRET system consists of luminol as chemiluminescent donor, and fluorescein isothiocyanate (FITC) as acceptor. The sensitive detection was achieved by using the system consisted of G-riched DNA, blocker DNA, and the Nb.BbvCI biocatalyst. Upon addition of target DNA to the system, target DNA hybridizes with the quasi-circular DNA structure, and forms a DNA duplex. The formation of DNA duplex triggers selective enzymatic cleavage of quasi-circular DNA by Nb.BbvCI, resulting in the release of target DNA and two G-riched DNAzyme segments. Released target DNA then hybridizes with another quasi-circular DNA structure to initiate the cleavage of the quasi-circular DNA structure. Eventually, each target DNA can go through many cycles, resulting in the digestion of many quasi-circular DNA structures, generating many G-riched DNAzyme segments. G-riched DNAzyme segment products assemble with hemin to form stable hemin/G-quadruplexes that exhibit peroxidase-like activity which can catalyze the oxidation of luminol by H2O2 to produce CL signals. In the presence of FITC, CL of luminol can excite FITC molecules, and thus produced CRET between the luminol and FITC. This unique analysis strategy gives a detection limit down to 80 fM, which is at least four orders of magnitude lower than that of unamplified DNA detection methods.

  10. Online detection of reactive oxygen species in ultraviolet (UV)-Irradiated nano-TiO2 suspensions by continuous flow chemiluminescence.

    Science.gov (United States)

    Wang, Dabin; Zhao, Lixia; Guo, Liang-Hong; Zhang, Hui

    2014-11-04

    Reactive oxygen species (ROS) play very important roles in the photocatalytic reactions of semiconductors. Using a continuous flow chemiluminescence (CFCL) system, we developed three methods for the selective, sensitive, and online detection of O2(• -), •OH, and H2O2 generated during ultraviolet (UV) irradiation of nano-TiO2 suspensions. TiO2 nanoparticles were irradiated in a photoreactor and pumped continuously into a detection cell. To detect O2(• -), luminol was mixed with TiO2 before it entered the detection cell. For the detection of short-lived •OH, phthalhydrazide was added into the photoreactor to capture •OH, and then mixed with H2O2/K5Cu(HIO6)2 to produce chemiluminescence (CL). To detect H2O2, an irradiated TiO2 suspension was kept in darkness for 30 min, and then mixed with luminol/K3Fe(CN)6 to produce CL. The selectivity of each method for a particular ROS was verified by using specific ROS scavengers. For a given ROS, a comparison between CL and conventional method showed good agreement for a series of TiO2 concentrations. The sensitivity of CL method was approximately 3-, 1200-, and 5-fold higher than the conventional method for O2(• -), •OH, and H2O2, respectively. To demonstrate the utility of the methods, ROS in three different types of TiO2 suspensions was detected by CFCL. It was found that photodegradation efficiency of Rhodamine B correlated the best (R(2) > 0.95) with the amount of photogenerated •OH, implying that •OH was the major oxidant in Rhodamine B photodegradation reaction. CFCL may provide a convenient tool for the studies on the reaction kinetics of ROS-participated decomposition of environmental contaminants.

  11. Flow injection chemiluminescence immunoassay of microcystin-LR by using PEI-modified magnetic beads as capturer and HRP-functionalized silica nanoparticles as signal amplifier.

    Science.gov (United States)

    Lu, Jusheng; Wei, Wei; Yin, Lihong; Pu, Yuepu; Liu, Songqin

    2013-03-07

    A rapid sandwiched immunoassay of microcystin-LR (MC-LR) in water is proposed with flow injection chemiluminescence detection. The magnetic beads (MBs) were first modified with polyethyleneimine (PEI) by acylamide bond between the carboxyl group on the surface of MBs and the primary amine group in PEI, followed by immobilizing of anti-MC-LR (Ab1) onto PEI with glutaraldehyde as linkage. The resulting Ab1 modified MBs captured the target MC-LR in water, reacted with the horseradish peroxidase and anti-MC-LR co-immobilized silica nanoparticles, and were detected with flow injection chemiluminescence. When using PEI/MBs as the carrier of anti-MC-LR, the CL signal was greatly enhanced up to 9-fold compared to that using MBs without PEI modification. The CL signal was further amplified 13-fold when Si/Ab2 was used as the signal probe. Under the optimal conditions, the present immunoassay exhibited a wide quantitative range from 0.02 to 200 μg L(-1) with a detection limit of 0.006 μg L(-1), which was much lower than the WHO provisional guideline limit of 1.0 μg L(-1) for MC-LR in drinking water. The relative standard deviation was 4.8% and the recoveries for the spiked samples ranged from 84% to 115%, which indicated acceptable precision and accuracy for MC-LR. The present method is easier to perform and less time-consuming (the entire analysis process lasted about 40 minutes) and has been applied to the detection of MC-LR in different water samples successfully.

  12. Suitability of thermoluminescence, chemiluminescence, ESR and viscosity measurements as detection method for the irradiation of medicinal herbs; Eignung von Thermolumineszenz-, Chemilumineszenz-, ESR- und Viskositaetsmessungen zur Identifizierung strahlenbehandelter Arzneidrogen

    Energy Technology Data Exchange (ETDEWEB)

    Schuettler, C.; Gebhardt, G.; Stock, A.; Helle, N.; Boegl, K.W.

    1993-12-31

    Chemiluminescence, electron spin resonance, thermoluminescence and viscosity measurements have been investigated for their suitability as detection method for the irradiation of the medicinal herbs anise seeds (anisi fructus), valerian roots (valerianae radix), redberry leaves (uvae ursi folium), birch leaves (betulae folium), greek hay seeds (foenugraeci semen), cayenne pepper (capsici fructus acer), black-aldertee bark (frangulae cortex), fennel fruits (feoniculi fructus), rose hip shells (cynosbati fructus), coltsfoot (farfarae folium), acorus roots (calami rhizoma), chamomile flowers (matricariae flos), caraway (carvi fructus), lavender flowers (lavandulae flos), linseed (lini semen), lime tree flowers (tiliae flos), St. Mary`s thistle fruit (cardui mariae herba), lemon balm (melissae folium), java tea (orthosiphonis folium), peppermint (menthae piperitae folium), sage leaves (salviae folium), scouring rush (equiseti herba), senna leaves (sennae folium), plantain herbs (plantaginis lanceolata herba), thyme herbs (thymi herba), juniper berries (juniperi fructus), hawthorne herbs (crataegi folium), wheat starch (amylum tritici) and wormwood (absinthii herba). Depending on the herbs, the methods used were more or less suitable. Chemiluminescence measurements showed the smallest differences between untreated and irradiated samples whereas thermoluminescence measurements on isolated minerals from the vegetable drugs gave better results. In some herbs radiation-specific radicals could be identified by ESR spectroscopy. Viscosity measurement is suitable for some herbs as fast and inexpensive method for screening. (orig.) [Deutsch] Die Eignung von Chemilumineszenz-, ESR-, Thermolumineszenz- und Viskositaetsmessungen als Nachweismethode fuer die Behandlung von Arzneidrogen mit ionisierenden Strahlen wurde an Anis, Baldrianwurzel, Baerentraubenblaettern, Birkenblaettern, Bockshornsamen, Cayennepfeffer, Faulbaumrinde, Fenchel, Hagebuttenschalen, Huflattichblaettern

  13. Enzyme-linked immunosorbent assay by enhanced chemiluminescence detection for the standardization of estrogenic miroestrol in Pueraria candollei Graham ex Benth.

    Science.gov (United States)

    Yusakul, Gorawit; Udomsin, Orapin; Tanaka, Hiroyuki; Morimoto, Satoshi; Juengwatanatrakul, Thaweesak; Putalun, Waraporn

    2015-08-01

    Miroestrol (ME) is a potent phytoestrogen from the P. candollei tuberous root. It has been approved for use in clinical trials due to its beneficial effect on disorders associated with estrogen deficiency. To ensure medical efficacy and safety, high performance analytical methods for ME analysis are required to standardize products from the P. candollei root. An enhanced chemiluminescence enzyme-linked immunosorbent assay (ECL-ELISA) was developed and validated using a polyclonal antibody against ME and a chemiluminescent system of luminol-H2 O2 -horseradish peroxidase-4-(1-imidazolyl) phenol. The ECL-ELISA system exhibited linearity over a concentration range of 0.31-10.00 ng mL(-1) , for which the relative standard variation (%RSD) was less than 10% for both intra- and interplate determinations. The ECL-ELISA is reliable for the determination of ME as reflected by the high recovery percentage (101.22-103.06%). As a comparative analysis, the ME content in each sample determined by ECL-ELISA was correlated with high coefficients of determination with colorimetric ELISA (R(2)  = 0.998) and high performance liquid chromatography (HPLC) (R(2)  = 0.998) methods. The ECL-ELISA method could be applied to all of the commercial products containing P. candollei root, when the products contain between 0.706 ± 0.046 and 13.123 ± 0.794 µg g(-1) dry wt. of ME. This method is useful as a high performance analytical method for the quantity control of ME in raw materials and end products at both the research and industrial levels.

  14. Development of a multichannel Fourier-transform spectrometer to measure weak chemiluminescence: Application to the emission of singlet-oxygen dimol in the decomposition of hydrogen peroxide with gallic acid and K 3[Fe(CN) 6

    Science.gov (United States)

    Tsukino, Kazuo; Satoh, Toshihiro; Ishii, Hiroshi; Nakata, Munetaka

    2008-05-01

    A Fourier-transform spectrometer equipped with a Savart-plate polarization interferometer was developed for observation of weak chemiluminescence and applied to a measurement of emission spectra in the decomposition of hydrogen peroxide with gallic acid and K 3[Fe(CN) 6]. The band appearing at ˜580 nm in the chemiluminescence spectrum was assigned to the emission of singlet-oxygen dimol, the peak wavelength being shifted from that observed in the reaction of hydrogen peroxide with sodium hypochlorite, ˜633 nm. The band intensity was increased with the increasing concentration of K 3[Fe(CN) 6] up to ˜100 mM, and thereafter the peak wavelength was shifted from 580 to 700 nm with a decrease in the intensity.

  15. 3-(10'-Phenothiazinyl)propionic acid is a potent primary enhancer of peroxidase-induced chemiluminescence and its application in sensitive ELISA of methylglyoxal-modified low density lipoprotein.

    Science.gov (United States)

    Sakharov, Ivan Yu; Demiyanova, Alexandra S; Gribas, Anastasia V; Uskova, Natalia A; Efremov, Evgeny E; Vdovenko, Marina M

    2013-10-15

    Using a full factorial design the optimization of experimental conditions of enhanced chemiluminescence reaction (ECR) catalyzed by horseradish peroxidase (HRP) in the presence of 3-(10'-phenothiazinyl)propionic acid (PPA) as a primary enhancer was performed. The effect of concentrations of PPA, hydrogen peroxide, MORPH, luminol, and Tris on a ratio of peroxidase-catalyzed CL to background was studied. The detection limit value of HRP in ECR with PPA was 0.09 pM. Using PPA the ultra-sensitive chemiluminescent ELISA for determination of methylglyoxal-modified low density lipoprotein was developed. The detection limit value for the developed method was 0.5 ng mL(-1). The obtained results open up very promising perspectives for using PPA to improve the sensitivity of enzyme immunoassay kits.

  16. The determination of psilocin and psilocybin in hallucinogenic mushrooms by HPLC utilizing a dual reagent acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection system.

    Science.gov (United States)

    Anastos, Nicole; Lewis, Simon W; Barnett, Neil W; Sims, D Noel

    2006-01-01

    This paper describes a procedure for the determination of psilocin and psilocybin in mushroom extracts using high-performance liquid chromatography with postcolumn chemiluminescence detection. A number of extraction methods for psilocin and psilocybin in hallucinogenic mushrooms were investigated, with a simple methanolic extraction being found to be most effective. Psilocin and psilocybin were extracted from a variety of hallucinogenic mushrooms using methanol. The analytes were separated on a C12 column using a (95:5% v/v) methanol:10 mM ammonium formate, pH 3.5 mobile phase with a run time of 5 min. Detection was realized through a dual reagent chemiluminescence detection system of acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II). The chemiluminescence detection system gave improved detectability when compared with UV absorption at 269 nm, with detection limits of 1.2 x 10(-8) and 3.5 x 10(-9) mol/L being obtained for psilocin and psilocybin, respectively. The procedure was applied to the determination of psilocin and psilocybin in three Australian species of hallucinogenic mushroom.

  17. Ultrasensitive detection of cancer cells and glycan expression profiling based on a multivalent recognition and alkaline phosphatase-responsive electrogenerated chemiluminescence biosensor

    Science.gov (United States)

    Chen, Xiaojiao; He, Yao; Zhang, Youyu; Liu, Meiling; Liu, Yang; Li, Jinghong

    2014-09-01

    A multivalent recognition and alkaline phosphatase (ALP)-responsive electrogenerated chemiluminescence (ECL) biosensor for cancer cell detection and in situ evaluation of cell surface glycan expression was developed on a poly(amidoamine) (PAMAM) dendrimer-conjugated, chemically reduced graphene oxide (rGO) electrode interface. In this strategy, the multivalency and high affinity of the cell-targeted aptamers on rGO provided a highly efficient cell recognition platform on the electrode. The ALP and concanavalin A (Con A) coated gold nanoparticles (Au NPs) nanoprobes allowed the ALP enzyme-catalyzed production of phenols that inhibited the ECL reaction of Ru(bpy)32+ on the rGO electrode interface, affording fast and highly sensitive ECL cytosensing and cell surface glycan evaluation. Combining the multivalent aptamer interface and ALP nanoprobes, the ECL cytosensor showed a detection limit of 38 CCRF-CEM cells per mL in human serum samples, broad dynamic range and excellent selectivity. In addition, the proposed biosensor provided a valuable insight into dynamic profiling of the expression of different glycans on cell surfaces, based on the carbohydrates recognized by lectins applied to the nanoprobes. This biosensor exhibits great promise in clinical diagnosis and drug screening.A multivalent recognition and alkaline phosphatase (ALP)-responsive electrogenerated chemiluminescence (ECL) biosensor for cancer cell detection and in situ evaluation of cell surface glycan expression was developed on a poly(amidoamine) (PAMAM) dendrimer-conjugated, chemically reduced graphene oxide (rGO) electrode interface. In this strategy, the multivalency and high affinity of the cell-targeted aptamers on rGO provided a highly efficient cell recognition platform on the electrode. The ALP and concanavalin A (Con A) coated gold nanoparticles (Au NPs) nanoprobes allowed the ALP enzyme-catalyzed production of phenols that inhibited the ECL reaction of Ru(bpy)32+ on the rGO electrode

  18. A novel chemiluminescence quenching method for determination of sulfonamides in pharmaceutical and biological fluid based on luminol-Ag(III) complex reaction in alkaline solution.

    Science.gov (United States)

    Sun, Hanwen; Chen, Peiyun; Li, Liqing; Cheng, Peng

    2011-05-01

    A novel chemiluminescence (CL) quenching method for the determination of sulfonamides is proposed. The CL reaction between Ag(III) complex [Ag(HIO₆)₂]⁵⁻ and luminol in alkaline solution was investigated. The quenching effect of sulfonamides on CL emission of [Ag(HIO₆)₂]⁵⁻-luminol system was found. Quenching degree of CL emission was proportional to sulfonamide concentration. The effects of the reaction conditions on CL emission and quenching were examined. Under optimal conditions, the detection limits (s/n = 3) were 7.2, 17 and 8.3 ng/mL for sulfadiazine, sulfameter, and sulfadimethoxine, respectively. The recoveries of the three drugs were in the range of 91.3-110% with RSDs of 1.9-2.7% for urine samples, and 106-112% with RSDs of 1.6-2.8% for serum samples. The proposed method was used for the determination of sulfadiazine at clinically relevant concentrations in real urine and serum samples with satisfactory results.

  19. Trivalent copper chelate-luminol chemiluminescence system for highly sensitive CE detection of dopamine in biological sample after clean-up using SPE.

    Science.gov (United States)

    Wang, Lin; Liu, Ying; Xie, Haoyue; Fu, Zhifeng

    2012-06-01

    A transition metal chelate unstable at a high oxidation state, diperiodatocuprate (III) (K₅[Cu(HIO₆)₂], DPC), was synthesized and applied in the luminol-based chemiluminescence (CL) system for highly sensitive CE end-column detection of dopamine (DA). This method was based on the fact that DA enhanced the CL emission resulting from the reaction between luminol and DPC in alkaline medium. The DPC-luminol-DA CL system showed very intensive emission and very fast kinetic characteristics, thus resulting in a high sensitivity in flow-through detection mode for CE. Under optimal conditions, the linear range was 1.0 × 10⁻⁸-5.0 × 10⁻⁵ g/mL (R² = 0.9984) with a limit of detection of 6.0 × 10⁻⁹ g/mL (S/N = 3). The RSDs of the peak height and the migration time were about 4.2 and 2.4% for a standard sample at 3.0 × 10⁻⁶ g/mL (n = 5), respectively. The presented method has been successfully used for the determination of DA in commercial preparation and human urine samples after clean-up using SPE.

  20. A flow injection chemiluminescence method for the determination of lincomycin in serum using a diperiodato-cuprate (III)-luminol system.

    Science.gov (United States)

    Hu, Yufei; Li, Gongke; Zhang, Zhujun

    2011-01-01

    In this paper, the novel trivalent copper-periodate complex {K₅[Cu(HIO₆)₂], DPC} has been applied in a luminol-based chemiluminescence (CL) reaction. Coupled with flow injection (FI) technology, the FI-CL method was proposed for the determination of lincomycin hydrochloride. The CL reaction between luminol and DPC occurred in an alkaline medium. The CL intensity could be greatly enhanced by lincomycin hydrochloride. The relative CL intensity was proportional to the concentration of lincomycin hydrochloride in the range of 1 × 10⁻⁸ to 5 × 10⁻⁶ g mL⁻¹ and the detection limit was at the 3.5 × 10⁻⁹ g mL⁻¹ level. The relative standard deviation at 5 × 10⁻⁸ g mL⁻¹ was 1.7% (n = 9). The sensitive method was successfully applied to the direct determination of lincomycin hydrochloride (ng mL⁻¹) in serum. A possible mechanism of the lumonol-DPC CL reaction was discussed by the study of the CL kinetic characteristics and the spectra of CL reaction. The oxidability of DPC was studied by means of its electrochemical response.

  1. Chemiluminescence immunoassay for the rapid and sensitive detection of antibody against porcine parvovirus by using horseradish peroxidase/detection antibody-coated gold nanoparticles as nanoprobes.

    Science.gov (United States)

    Zhou, Yuan; Zhou, Tao; Zhou, Rui; Hu, Yonggang

    2014-06-01

    A rapid, simple, facile, sensitive and enzyme-amplified chemiluminescence immunoassay (CLIA) method to detect antibodies against porcine parvovirus has been developed. Horseradish peroxidase (HRP) and the detection antibody were simultaneously co-immobilized on the surface of gold nanoparticles using the electrostatic method to form gold nanoparticle-based nanoprobes. This nanoprobe was employed in a sandwich-type CLIA, which enables CL signal readout from enzymatic catalysis and results in signal amplification. The presence of porcine parvovirus infection was determined in porcine parvovirus antibodies by measuring the CL intensity caused by the reaction of HRP-luminol with H2 O2 . Under optimal conditions, the obtained calibration plot for the standard positive serum was approximately linear within the dilution range of 1:80 to 1:5120. The limit of detection for the assay was 1:10,240 (S/N = 3), which is much lower than that typically achieved with an enzyme-linked immunosorbent assay (1:160; S/N = 3). A series of repeatability measurements using 1:320-fold diluted standard positive serum gave reproducible results with a relative standard deviation of 4.9% (n = 11). The ability of the immunosensor to analyze clinical samples was tested on porcine sera. The immunosensor had an efficiency of 90%, a sensitivity of 93.3%, and a specificity of 87.5% relative to the enzyme-linked immunosorbent assay results.

  2. Homogenous electrogenerated chemiluminescence immunoassay for human immunoglobulin G using N-(aminobutyl)-N-ethylisoluminol as luminescence label at gold nanoparticles modified paraffin-impregnated graphite electrode.

    Science.gov (United States)

    Qi, Honglan; Zhang, Yi; Peng, Yage; Zhang, Chengxiao

    2008-05-15

    A homogeneous electrogenerated chemiluminescence (ECL) immunoassay for human immunoglobulin G (hIgG) has been developed using a N-(aminobutyl)-N-ethylisoluminol (ABEI) as luminescence label at gold nanoparticles modified paraffin-impregnated graphite electrode (PIGE). ECL emission was electrochemically generated from the ABEI-labeled anti-hIgG antibody and markedly increased in the presence of hIgG antigen due to forming a more rigid structure of the ABEI moiety. The concentration of hIgG antigen was determined by the increase of ECL intensity at a gold nanoparticles modified PIGE. It was found that the ECL intensity of ABEI in presence of hydrogen peroxide was dramatically enhanced at gold nanoparticles modified PIGE in neutral aqueous solution and the detection limit of ABEI was 2 x 10(-14)mol/L (S/N=3). The integral ECL intensity was linearly related to the concentration of hIgG antigen from 3.0 x 10(-11) to 1.0 x 10(-9)g/mL with a detection limit of 1 x 10(-11)g/mL (S/N=3). The relative standard deviation was 3.1% at 1.0 x 10(-10)g/mL (n=11). This work demonstrates that the enhancement of the sensitivity of ECL and ECL immunoassay at a nanoparticles modified electrode is a promising strategy.

  3. Interfacing on-line solid phase extraction with monolithic column multisyringe chromatography and chemiluminescence detection: An effective tool for fast, sensitive and selective determination of thiazide diuretics.

    Science.gov (United States)

    Maya, Fernando; Estela, José Manuel; Cerdà, Víctor

    2010-01-15

    A new, multisyringe flow injection set-up has been developed for the completely automated determination of trace thiazide compounds with diuretic action in different types of samples. The proposed instrumental set-up exploits for the first time, a low pressure on-line solid phase extraction-liquid chromatography-chemiluminescence detection method. This novel combination of sample treatments in flow systems expands the current applicability of low pressure liquid chromatography due to the isolation/preconcentration of the target compounds, besides high selectivity and sensitivity. For the determination of three thiazide compounds named hydroflumethiazide, furosemide and bendroflumethiazide, the proposed set-up provided with the preconcentration of only 1mL of sample, limits of detection of 3, 60 and 40microgL(-1), respectively. Furthermore wide linear dynamic ranges of 6-4000, 140-20,000 and 90-40,000microgL(-1), respectively, were obtained. Besides of this, a high injection throughput of 12h(-1) was also achieved. As in sports, thiazide diuretics are prohibited substances, the proposed method has been applied to their determination in urine samples. Furthermore the potential of the proposed method as a fast-screening approach for emerging contaminants in waters has been also tested by applying it to well water and leachates from a solid waste landfill.

  4. A simple and rapid flow-injection chemiluminescence method for the determination of noscapine with Ru(phen)3(2+)-Ce(IV) system.

    Science.gov (United States)

    Rezaei, Behzad; Mokhtari, Ali; Khayamian, Taghi

    2007-08-01

    A new flow injection chemiluminescence (CL) system was used for the determination of noscapine. This technique is based on the reduction effect of noscapine on the Ru(phen)3(3+), which is produced by reaction between Ru(phen)3(2+) and acidic Ce(IV) solutions, and this rapid reduction produces strong CL. Calibration plots were linear over the range of 3.0 x 10(-7) - 2.0 x 10(-6) mol L(-1) and 2.0 x 10(-6) - 2.0 x 10(-4) mol L(-1). The CL intensity was so high, that it is able to produce a detection limit of 6.6 x 10(-8) M noscapine (3sigma). The relative standard deviation of 2.0 x 10(-6) M noscapine was 1.0% (n=10). The proposed method was successfully applied for the flow injection determination of noscapine in cough and Tonin syrup samples. The results of real sample analyses show good recovery percentages (97.3-102.4%). The minimum sampling rate was 100 samples per hour.

  5. A new approach for bisphenol A detection employing fluorosurfactant-capped gold nanoparticle-amplified chemiluminescence from cobalt(II) and peroxymonocarbonate.

    Science.gov (United States)

    Pan, Feng; Liu, Lin; Dong, Shichao; Lu, Chao

    2014-07-15

    In this work, we utilized the nonionic fluorosurfactant-capped gold nanoparticles (GNPs) as a novel chemiluminescence (CL) probe for the determination of trace bisphenol A. Bisphenol A can induce a sharp decrease in CL intensity from the GNP-Co(2+)-peroxymonocarbonate (HCO4(-)) system. Under the selected experimental conditions, a linear relationship was obtained between the CL intensity and the logarithm of concentration of bisphenol A in the range of 0.05-50 μM (R(2) = 0.9936), and the detection limit at a signal-to-noise ratio of 3 for bisphenol A was 10 nM. The applicability of the proposed method has been validated by determining bisphenol A in real polycarbonate samples with satisfactory results. The recoveries for bisphenol A in spiked samples were found to be between 94.4% and 105.0%. The relative standard deviation (RSD) for 12 repeated measurements of 0.5 μM bisphenol A was 2.2%. The proposed method described herein was simple, selective and obviated the need of extensive sample pretreatment.

  6. Simultaneous identification and quantification of new psychoactive substances in blood by GC-APCI-QTOFMS coupled to nitrogen chemiluminescence detection without authentic reference standards.

    Science.gov (United States)

    Ojanperä, Ilkka; Mesihää, Samuel; Rasanen, Ilpo; Pelander, Anna; Ketola, Raimo A

    2016-05-01

    A novel platform is introduced for simultaneous identification and quantification of new psychoactive substances (NPS) in blood matrix, without the necessity of using authentic reference standards. The instrumentation consisted of gas chromatography (GC) coupled to nitrogen chemiluminescence detection (NCD) and atmospheric pressure chemical ionization quadrupole time-of-flight mass spectrometry (APCI-QTOFMS). In this concept, the GC flow is divided in appropriate proportions between NCD for single-calibrant quantification, utilizing the detector's equimolar response to nitrogen, and QTOFMS for accurate mass-based identification. The principle was proven by analyzing five NPS, bupropion, desoxypipradrol (2-DPMP), mephedrone, methylone, and naphyrone, in sheep blood. The samples were spiked with the analytes post-extraction to avoid recovery considerations at this point. All the NPS studies produced a protonated molecule in APCI resulting in predictable fragmentation with high mass accuracy. The N-equimolarity of quantification by NCD was investigated by using external calibration with the secondary standard caffeine at five concentration levels between 0.17 and 1.7 mg/L in blood matrix as five replicates. The equimolarity was on average 98.7%, and the range of individual equimolarity determinations was 76.7-130.1%. The current analysis platform affords a promising approach to instant simultaneous qualitative and quantitative analysis of drugs in the absence of authentic reference standards, not only in forensic and clinical toxicology but also in other bioanalytical applications.

  7. Development of a rapid and high-performance chemiluminescence immunoassay based on magnetic particles for protein S100B in human serum.

    Science.gov (United States)

    Zhang, Huisheng; Qi, Suwen; Rao, Jie; Li, Qiaoliang; Yin, Li; Lu, Yuejun

    2013-01-01

    Protein S100B is a clinically useful non-invasive biomarker for brain cell damage. A rapid chemiluminescence immunoassay (CLIA) for S100B in human serum has been developed. Fluorescein isothiocyanate (FITC) and N-(aminobutyl)-N-(ethylisoluminol) (ABEI) are used to label two different monoclonal antibodies of anti-S100B. Protein S100B in serum combines with labeled antibodies and can form a sandwiched immunoreaction. A simplified separation procedure based on the use of magnetic particles (MPs) that were coated with anti-FITC antibody is performed to remove the unwanted materials. After adding the substrate solution, the relative light unit (RLU) of ABEI is measured and is found to be directly proportional to the concentration of S100B in serum. The relevant variables involved in the CLIA signals are optimized and the parameters of the proposed method are evaluated. The results demonstrate that the method is linear to 25 ng/mL S100B with a detection limit of 0.02 ng/mL. The coefficient of variation (CV) is < 5% and < 6% for intra- and interassay precision, respectively. The average recoveries are between 97 and 107%. The linearity-dilution effect produces a linear correlation coefficient of 0.9988. Compared with the commercial kit, the proposed method shows a correlation of 0.9897. The proposed method displays acceptable performance for quantification of S100B and is appropriate for use in clinical diagnosis.

  8. Alterations in luminol-enhanced chemiluminescence from nondiluted whole blood in the course of low-level laser therapy of angina pectoris patients

    Science.gov (United States)

    Voeikov, Vladimir L.; Novikov, Cyril N.; Siuch, Natalia I.

    1997-05-01

    Addition of Luminol to nondiluted blood of healthy donors results in a short and weak increase of chemiluminescence (CL) from it. Contrary to that in 25 cases of stable angina pectoris the intensity of CL from blood of patients sharply increased upon addition of luminol exceeding that form healthy donors' blood 10-100-fold. 24 hours after the 3D intravenous low-level treatment CL burst in patients' blood in the presence of Luminol was in general significantly lower than before the beginning of the treatment. After the 7th treatment the pattern of CL kinetics was in most cases similar to that of healthy donors' blood. However, after the 10th treatment intensity of Luminol-enhanced CL usually increased and for blood of some patients even exceeded its values obtained before the treatment. Some correlation CL from nondiluted blood with neutrophil activity studied by NTB-test and plasma viscosity of same blood was noted. Using highly sensitive single photon counters it is possible to reveal abnormal levels of CL from no more than 0.1-0.2 ml of blood within 3-5 min.

  9. Regenerable immuno-biochip for screening ochratoxin A in green coffee extract using an automated microarray chip reader with chemiluminescence detection.

    Science.gov (United States)

    Sauceda-Friebe, Jimena C; Karsunke, Xaver Y Z; Vazac, Susanna; Biselli, Scarlett; Niessner, Reinhard; Knopp, Dietmar

    2011-03-18

    Ochratoxin A (OTA) can contaminate foodstuffs in the ppb to ppm range and once formed, it is difficult to remove. Because of its toxicity and potential risks to human health, the need exists for rapid, efficient detection methods that comply with legal maximum residual limits. In this work we have synthesized an OTA conjugate functionalized with a water-soluble peptide for covalent immobilization on a glass biochip by means of contact spotting. The chip was used for OTA determination with an indirect competitive immunoassay format with flow-through reagent addition and chemiluminescence detection, carried out with the stand-alone automated Munich Chip Reader 3 (MCR 3) platform. A buffer model and real green coffee extracts were used for this purpose. At the present, covalent conjugate immobilization allowed for at least 20 assay-regeneration cycles of the biochip surface. The total analysis time for a single sample, including measurement and surface regeneration, was 12 min and the LOQ of OTA in green coffee extract was 0.3 μg L(-1) which corresponds to 7 μg kg(-1).

  10. An ultra-facile and label-free immunoassay strategy for detection of copper (II) utilizing chemiluminescence self-enhancement of Cu (II)-ethylenediaminetetraacetate chelate.

    Science.gov (United States)

    Ouyang, Hui; Shu, Qi; Wang, Wenwen; Wang, Zhenxing; Yang, Shijia; Wang, Lin; Fu, Zhifeng

    2016-11-15

    The establishment of facile, rapid, sensitive and cost-effective protocols for the detection of heavy metals is of great significance for human health and environmental monitoring. Hereby, an ultra-facile and label-free immunoassay strategy was designed for detecting heavy metal ion by using Cu (II) as the model analyte. Cu (II) reacted previously with ethylenediaminetetraacetate (EDTA) was captured by immobilized monoclonal antibody for Cu (II)-EDTA chelate. Then Cu (II) was detected based on the self-enhancing effect of Cu (II)-EDTA chelate to luminol-H2O2 chemiluminescence reaction. The CL intensity is linear relative with Cu (II) concentration in a very wide range of 1.0-1000ng/mL, with a detection limit of 0.33ng/mL (S/N=3). Since the specificity of this proposed strategy relied on both the specificity of monoclonal antibody and the specificity of luminol-H2O2 system, it could avoid interference from most common ions. The proposed method was used successfully to detect Cu (II) in traditional Chinese medicine and environmental water samples with acceptable recovery values of 82-113%. This proof-of-principle work demonstrated the feasibility of the label-free immunoassay for heavy metal ions, and opened a new avenue for rapid screening and field assay for drug safety, environmental monitoring and clinical diagnosis.

  11. A new approach for bisphenol A detection employing fluorosurfactant-capped gold nanoparticle-amplified chemiluminescence from cobalt(II) and peroxymonocarbonate

    Science.gov (United States)

    Pan, Feng; Liu, Lin; Dong, Shichao; Lu, Chao

    2014-07-01

    In this work, we utilized the nonionic fluorosurfactant-capped gold nanoparticles (GNPs) as a novel chemiluminescence (CL) probe for the determination of trace bisphenol A. Bisphenol A can induce a sharp decrease in CL intensity from the GNP-Co2+-peroxymonocarbonate (HCO4-) system. Under the selected experimental conditions, a linear relationship was obtained between the CL intensity and the logarithm of concentration of bisphenol A in the range of 0.05-50 μM (R2 = 0.9936), and the detection limit at a signal-to-noise ratio of 3 for bisphenol A was 10 nM. The applicability of the proposed method has been validated by determining bisphenol A in real polycarbonate samples with satisfactory results. The recoveries for bisphenol A in spiked samples were found to be between 94.4% and 105.0%. The relative standard deviation (RSD) for 12 repeated measurements of 0.5 μM bisphenol A was 2.2%. The proposed method described herein was simple, selective and obviated the need of extensive sample pretreatment.

  12. Fluorosurfactant-prepared triangular gold nanoparticles as postcolumn chemiluminescence reagents for high-performance liquid chromatography assay of low molecular weight aminothiols in biological fluids.

    Science.gov (United States)

    Li, Qianqian; Shang, Fei; Lu, Chao; Zheng, Zhixia; Lin, Jin-Ming

    2011-12-16

    Our recent study demonstrates the synthesized triangular gold nanoparticles (AuNPs) by trisodium citrate reduction of HAuCl(4) in the presence of nonionic fluorosurfactant (FSN) could display stronger catalytic activity towards luminol-chemiluminescence (CL) than spherical AuNPs. Ultratrace aminothiols may cause a great decrease in CL intensity of the triangular AuNPs-luminol CL system. In this article, we utilize the as-prepared triangular AuNPs as novel postcolumn CL reagents to explore a simple high-performance liquid chromatography (HPLC)-CL method for the determination of low molecular weight aminothiols (i.e., cysteine, homocysteine, glutathione, cysteinylglycine and glutamylcysteine). The as-prepared triangular AuNPs were easier to synthesize, stable at a wider pH range and high ionic strength, and highly selective and sensitive towards reduced aminothiols. The detection limits at a signal-to-noise ratio of 3 for cysteine, homocysteine, glutathione, cysteinylglycine and glutamylcysteine were 0.016, 0.08, 0.1, 0.04 and 0.1pmol, respectively. Recoveries from spiked urine and plasma samples were 95.7-104.3%. The applicability of the proposed method has been validated by determining these low molecular weight aminothiols in human urine and plasma samples with satisfactory results, and thus it will have great potential application in clinical diagnosis.

  13. Determination of nitrite in tap waters based on fluorosurfactant-capped gold nanoparticles-enhanced chemiluminescence from carbonate and peroxynitrous acid.

    Science.gov (United States)

    Li, Jinge; Li, Qianqian; Lu, Chao; Zhao, Lixia

    2011-06-07

    Fluorosurfactant (FSN)-capped gold nanoparticles (GNPs), which exhibit higher stability at a wider pH range and high ionic strength, were utilized for investigating the chemiluminescence (CL) effect on the reaction between high concentration carbonate (~0.3 M) and peroxynitrous acid. When the pH of the colloidal solution was 10.2 by dropwise addition of 0.05 M NaOH, FSN-capped GNPs offer an enhanced CL intensity. Based on the CL spectra, XPS spectra and the quenching effect of reactive oxygen species, a possible CL mechanism is proposed. The tolerance of FSN-capped GNPs towards salt concentrations (salt proofing effect) within a large pH range is an interesting feature, compared to the other previously reported work on nanoparticles CL systems. The CL intensity is proportional to the concentration of nitrite in the range from 0.1 to 100 μM. The detection limit (S/N = 3) is 0.036 μM and the relative standard deviation (RSD) for seven repeated measurements of 0.5 μM nitrite was 2.4%. This method has been successfully applied to determine nitrite in tap waters with recoveries of 97-106%.

  14. Investigation of iron(III) reduction and trace metal interferences in the determination of dissolved iron in seawater using flow injection with luminol chemiluminescence detection

    Energy Technology Data Exchange (ETDEWEB)

    Ussher, Simon J. [School of Earth, Ocean and Environmental Sciences (SEOES), University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Milne, Angela [School of Earth, Ocean and Environmental Sciences (SEOES), University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Department of Oceanography, Florida State University, Tallahassee, FL 32306-4320 (United States); Landing, William M. [Department of Oceanography, Florida State University, Tallahassee, FL 32306-4320 (United States); Attiq-ur-Rehman, Kakar [Department of Chemistry, University of Balochistan, Quetta (Pakistan); Seguret, Marie J.M.; Holland, Toby [School of Earth, Ocean and Environmental Sciences (SEOES), University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Achterberg, Eric P. [National Oceanography Centre, University of Southampton, European Way, Southampton SO14 3ZH (United Kingdom); Nabi, Abdul [Department of Chemistry, University of Balochistan, Quetta (Pakistan); Worsfold, Paul J., E-mail: pworsfold@plymouth.ac.uk [School of Earth, Ocean and Environmental Sciences (SEOES), University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom)

    2009-10-12

    A detailed investigation into the performance of two flow injection-chemiluminescence (FI-CL) manifolds (with and without a preconcentration column) for the determination of sub-nanomolar dissolved iron (Fe(II) + Fe(III)), following the reduction of Fe(III) by sulphite, in seawater is described. Kinetic experiments were conducted to examine the efficiency of reduction of inorganic Fe(III) with sulphite under different conditions and a rigorous study of the potential interference caused by other transition metals present in seawater was conducted. Using 100 {mu}M concentrations of sulphite a reduction time of 4 h was sufficient to quantitatively reduce Fe(III) in seawater. Under optimal conditions, cobalt(II) and vanadium(IV)/(III) were the major positive interferences and strategies for their removal are reported. Specifically, cobalt(II) was masked by the addition of dimethylglyoxime to the luminol solution and vanadium(IV) was removed by passing the sample through an 8-hydroxyquinoline column in a low pH carrier stream. Manganese(II) also interfered by suppression of the CL response but this was not significant at typical open ocean concentrations.

  15. Sensitive determination method for mercury ion, methyl-, ethyl-, and phenyl-mercury in water and biological samples using high-performance liquid chromatography with chemiluminescence detection.

    Science.gov (United States)

    Kodamatani, Hitoshi; Matsuyama, Akito; Saito, Keiitsu; Kono, Yuriko; Kanzaki, Ryo; Tomiyasu, Takashi

    2012-01-01

    A sensitive determination method for mercury speciation analysis was developed. Four mercury species, mercury ion, methylmercury, ethylmercury, and phenylmercury, were complexed with emetine-dithiocarbamate (emetine-CS(2)), and then injected onto a HPLC instrument coupled with a tris(2,2'-bipyridine)ruthenium(III) chemiluminescence detection system. The emetine-CS(2) complexing agent was effectively used to measure the concentration in addition to serving as a separation and detection reagent. The calibration curves for these mercury complexes were linear in the range of 0.050 - 10 μg L(-1) (as Hg). The limit of detection for (emetine-CS(2))(2)Hg, emetine-CS(2)-methylmercury, emetine-CS(2)-ethylmercury, and emetine-CS(2)-phenylmercury were 30, 17, 21, and 22 ng L(-1), respectively. The sensitivity of this method enables the determination of mercury species in water samples at sub-ppb levels. Furthermore, the method was applied to biological samples in combination with acid leaching and liquid-liquid extraction using emetine-CS(2) as an extraction reagent. The determination results were in good agreement with the values of the certified reference materials.

  16. 3D origami-based multifunction-integrated immunodevice: low-cost and multiplexed sandwich chemiluminescence immunoassay on microfluidic paper-based analytical device.

    Science.gov (United States)

    Ge, Lei; Wang, Shoumei; Song, Xianrang; Ge, Shenguang; Yu, Jinghua

    2012-09-07

    A novel 3D microfluidic paper-based immunodevice, integrated with blood plasma separation from whole blood samples, automation of rinse steps, and multiplexed CL detections, was developed for the first time based on the principle of origami (denoted as origami-based device). This 3D origami-based device, comprised of one test pad surrounded by four folding tabs, could be patterned and fabricated by wax-printing on paper in bulk. In this work, a sandwich-type chemiluminescence (CL) immunoassay was introduced into this 3D origami-based immunodevice, which could separate the operational procedures into several steps including (i) folding pads above/below and (ii) addition of reagent/buffer under a specific sequence. The CL behavior, blood plasma separation, washing protocol, and incubation time were investigated in this work. The developed 3D origami-based CL immunodevice, combined with a typical luminuol-H(2)O(2) CL system and catalyzed by Ag nanoparticles, showed excellent analytical performance for the simultaneous detection of four tumor markers. The whole blood samples were assayed and the results obtained were in agreement with the reference values from the parallel single-analyte test. This paper-based microfluidic origami CL detection system provides a new strategy for a low-cost, sensitive, simultaneous multiplex immunoassay and point-of-care diagnostics.

  17. Highly sensitive chemiluminescence immunoassay on chitosan membrane modified paper platform using TiO2 nanoparticles/multiwalled carbon nanotubes as label.

    Science.gov (United States)

    Li, Weiping; Ge, Shenguang; Wang, Shoumei; Yan, Mei; Ge, Lei; Yu, Jinghua

    2013-01-01

    A highly sensitive chemiluminescence (CL) immunoassay was incorporated into a low-cost microfluidic paper-based analytical device (μ-PAD) to fabricate a facile paper-based CL immunodevice (denoted as μ-PCLI). This μ-PCLI was constructed by covalently immobilizing capture antibody on a chitosan membrane modified μ-PADs, which was developed by simple wax printing methodology. TiO2 nanoparticles coated multiwalled carbon nanotubes (TiO2/MWCNTs) were synthesized as an amplification catalyst tag to label signal antibody (Ab2). After sandwich-type immunoreactions, the TiO2/MWCNTs were captured on the surface of μ-PADs to catalyze the luminol-p-iodophenol-H2O2 CL system, which produced an enhanced CL emission. Using prostate-specific antigen as a model analyte, the approach provided a good linear response range from 0.001 to 20 ng/mL with a low detection limit of 0.8 pg/mL under optimal conditions. This μ-PCLI showed good reproducibility, selectivity and stability. The assay results of prostate-specific antigen in clinical serum samples were in good agreement with that obtained by commercially used electrochemiluminescence methods at the Cancer Research Center of Shandong Tumor Hospital (Jinan, Shandong Province, China). This μ-PCLI could be very useful to realize highly sensitive, qualitative point-of-care testing in developing or developed countries.

  18. Screening of cannabinoids in industrial-grade hemp using two-dimensional liquid chromatography coupled with acidic potassium permanganate chemiluminescence detection.

    Science.gov (United States)

    Pandohee, Jessica; Holland, Brendan J; Li, Bingshan; Tsuzuki, Takuya; Stevenson, Paul G; Barnett, Neil W; Pearson, James R; Jones, Oliver A H; Conlan, Xavier A

    2015-06-01

    Widely known for its recreational use, the cannabis plant also has the potential to act as an antibacterial agent in the medicinal field. The analysis of cannabis plants/products in both pharmacological and forensic studies often requires the separation of compounds of interest and/or accurate identification of the whole cannabinoid profile. In order to provide a complete separation and detection of cannabinoids, a new two-dimensional liquid chromatography method has been developed using acidic potassium permanganate chemiluminescence detection, which has been shown to be selective for cannabinoids. This was carried out using a Luna 100 Å CN column and a Poroshell 120 EC-C18 column in the first and second dimensions, respectively. The method has utilized a large amount of the available separation space with a spreading angle of 48.4° and a correlation of 0.66 allowing the determination of more than 120 constituents and mass spectral identification of ten cannabinoids in a single analytical run. The method has the potential to improve research involved in the characterization of sensitive, complex matrices.

  19. A novel flow-injection method for the determination of Pt(IV) in environmental samples based on chemiluminescence reaction of lucigenin and biosorption.

    Science.gov (United States)

    Malejko, Julita; Godlewska-Zyłkiewicz, Beata; Kojło, Anatol

    2010-06-15

    A new flow-injection chemiluminescence method (FI-CL) was developed for the determination of trace amounts of Pt(IV). The method is based on the quenching effect of the analyte on CL emission generated by lucigenin in alkaline solution. Application of a column filled with an algae Chlorella vulgaris immobilized on Cellex-T resin allowed to preconcentrate and separate the Pt(IV) ions from complex environmental samples, such as road dust. The developed method is simple and does not require sophisticated instrumentation. It is also characterized by a very low limit of detection (0.1ngmL(-1)), good sensitivity and precision (RSD<3%). The accuracy of the presented method was confirmed by analysis of a certified reference material of tunnel dust (BCR-723). The content of Pt in road dust samples collected in Białystok (Poland) in 2009 determined by the evaluated method was 351.8+/-54.6ngg(-1) and was higher than in samples collected in years 2000 and 2003.

  20. Chemiluminescence of Mn-Doped ZnS Nanocrystals Induced by Direct Chemical Oxidation and Ionic Liquid-Sensitized Effect as an Efficient and Green Catalyst

    Directory of Open Access Journals (Sweden)

    Seyed Naser Azizi

    2013-01-01

    Full Text Available A novel chemiluminescence (CL method was proposed for doping water-soluble Mn in ZnS quantum dots (QDs as CL emitter. Water-soluble Mn-doped ZnS QDs were synthesized by using L-cysteine as stabilizer in aqueous solution. These nanoparticles were structurally and optically characterized by X-ray powder diffraction (XRD, dynamic light scattering (DLS, Fourier transform infrared spectroscopy (FTIR, UV-Vis absorption spectroscopy, and photoluminescence (PL emission spectroscopy. The CL of ZnS QDs was induced directly by chemical oxidation and its ionic liquid-sensitized effect in aqueous solution was then investigated. It was found that oxidants, especially hydrogen peroxide, could directly oxidize ZnS QDs to produce weak CL emission in basic solutions. In the presence of 1,3-dipropylimidazolium bromide/copper, a drastic light emission enhancement was observed which is related to a strong interaction between Cu2+ and the imidazolium ring. In these conditions, an efficient CL light was produced at low pH which is suggested to be beneficial to the biological analysis. The CL properties of QDs not only will be helpful to study physical chemistry properties of semiconductor nanocrystals but also they are expected to find use in many fields such as luminescence devices, bioanalysis, and multicolor labeling probes.

  1. An Investigation of the Effect of Charge Inhomogeneity on the Ignition and Combustion Processes in a HCCI Engine Using Chemiluminescence Imaging

    Science.gov (United States)

    Kumano, Kengo; Yamasaki, Yudai; Iida, Norimasa

    In the HCCI (Homogeneous Charge Compression Ignition) engines, inhomogeneity in fuel distribution and temperature in the pre-mixture exists microscopically and has possibility to affect the ignition and combustion process. In this study, the effect of charge inhomogeneity in fuel distribution on the HCCI combustion process was investigated. Pressure profiles were measured and two dimensional chemiluminescence images were captured by using a framing camera with a 4-stroke optically accessible engine in order to understand the spatial distribution of the combustion. DME (di-methyl ether) was used as the test fuel. By changing the way of mixing air and fuel in the intake manifold, inhomogeneity in fuel distribution in the pre-mixture was varied. The result shows that luminescence is observed in a very short time in a large part of the combustion chamber under the homogeneous condition, while luminescence appears locally with considerable time differences under the inhomogeneous condition. It is also shown that the local luminescence durations are almost the same under both conditions.

  2. CuO nanosheets-enhanced flow-injection chemiluminescence system for determination of vancomycin in water, pharmaceutical and human serum

    Science.gov (United States)

    Khataee, A. R.; Hasanzadeh, A.; Iranifam, M.; Fathinia, M.; Hanifehpour, Y.; Joo, S. W.

    2014-03-01

    A novel, rapid and sensitive CuO nanosheets (NSs) amplified flow-injection chemiluminescence (CL) system, luminol-H2O2-CuO nanosheets, was developed for determination of the vancomycin hydrochloride for the first time. It was found that vancomycin could efficiently inhibit the CL intensity of luminol-H2O2-CuO nanosheets system in alkaline medium. Under the optimum conditions, the inhibited CL intensity was linearly proportional to the concentration of vancomycin over the ranges of 0.5-18.0 and 18.0-40.0 mg L-1, with a detection limit (3σ) of 0.1 mg L-1. The precision was calculated by analyzing samples containing 5.0 mg L-1 vancomycin (n = 11) and the relative standard deviation (RSD) was 2.8%. Also, a high injection throughput of 120 sample h-1 was obtained. The CuO nanosheets were synthesized by a sonochemical method. Also, X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses were employed to characterize the CuO nanosheets. The method was successfully employed to determine vancomycin hydrochloride in environmental water samples, pharmaceutical formulation and spiked human serum.

  3. Chemiluminescent labels released from long spacer arm-functionalized magnetic particles: a novel strategy for ultrasensitive and highly selective detection of pathogen infections.

    Science.gov (United States)

    Yang, Haowen; Liang, Wenbiao; He, Nongyue; Deng, Yan; Li, Zhiyang

    2015-01-14

    Previously, the unique advantages provided by chemiluminescence (CL) and magnetic particles (MPs) have resulted in the development of many useful nucleic acid detection methods. CL is highly sensitive, but when applied to MPs, its intensity is limited by the inner filter-like effect arising from excess dark MPs. Herein, we describe a modified strategy whereby CL labels are released from MPs to eliminate this negative effect. This approach relies on (1) the magnetic capture of target molecules on long spacer arm-functionalized magnetic particles (LSA-MPs), (2) the conjugation of streptavidin-alkaline phosphatase (SA-AP) to biotinylated amplicons of target pathogens, (3) the release of CL labels (specifically, AP tags), and (4) the detection of the released labels. CL labels were released from LSA-MPs through LSA ultrasonication or DNA enzymolysis, which proved to be the superior method. In contrast to conventional MPs, LSA-MPs exhibited significantly improved CL detection, because of the introduction of LSA, which was made of water-soluble carboxymethylated β-1,3-glucan. Detection of hepatitis B virus with this technique revealed a low detection limit of 50 fM, high selectivity, and excellent reproducibility. Thus, this approach may hold great potential for early stage clinical diagnosis of infectious diseases.

  4. Chemiluminescent detection of sequential DNA hybridizations to high-density, filter-arrayed cDNA libraries: a subtraction method for novel gene discovery.

    Science.gov (United States)

    Guiliano, D; Ganatra, M; Ware, J; Parrot, J; Daub, J; Moran, L; Brennecke, H; Foster, J M; Supali, T; Blaxter, M; Scott, A L; Williams, S A; Slatko, B E

    1999-07-01

    A chemiluminescent approach for sequential DNA hybridizations to high-density filter arrays of cDNAs, using a biotin-based random priming method followed by a streptavidin/alkaline phosphatase/CDP-Star detection protocol, is presented. The method has been applied to the Brugia malayi genome project, wherein cDNA libraries, cosmid and bacterial artificial chromosome (BAC) libraries have been gridded at high density onto nylon filters for subsequent analysis by hybridization. Individual probes and pools of rRNA probes, ribosomal protein probes and expressed sequence tag probes show correct specificity and high signal-to-noise ratios even after ten rounds of hybridization, detection, stripping of the probes from the membranes and rehybridization with additional probe sets. This approach provides a subtraction method that leads to a reduction in redundant DNA sequencing, thus increasing the rate of novel gene discovery. The method is also applicable for detecting target sequences, which are present in one or only a few copies per cell; it has proven useful for physical mapping of BAC and cosmid high-density filter arrays, wherein multiple probes have been hybridized at one time (multiplexed) and subsequently "deplexed" into individual components for specific probe localizations.

  5. Comparison analysis between Western blot method and Magnetic particle Chemiluminescence method detection SLE autoantibody%免疫印迹法和磁微粒化学发光法检测SLE自身抗体的比对分析

    Institute of Scientific and Technical Information of China (English)

    周厚清

    2016-01-01

    目的:比较免疫印迹法(Western blot)和磁微粒化学发光法(Magnetic particle Chemiluminescence method)检测系统性红斑狼疮(SLE)自身抗体的一致性。方法:用免疫印迹法和磁微粒化学发光法对123份血清进行检测,其中系统性红斑狼疮阳性患者63例,健康体检者60例,用卡方检验对两种方法结果进行比较分析。结果:免疫印迹法和磁微粒化学发光法中抗Sm抗体阳性率分别为27.0%和14.3%、抗RNP/Sm阳性率分别为54.0%和42.9%、dsDNA阳性率分别为41.3%和30.2%、抗核小体抗体阳性率分别为31.7%和20.6%、抗核糖体P蛋白抗体阳性率分别为28.6%和11.1%。两种方法的准确性无显著性差异(>0.05)总符合率为80.6%,Kappa值为0.669。结论:免疫印迹法和磁微粒化学发光法检测系统性红斑狼疮自身抗体的检测结果具有可靠性,较好的符合率和准确性,磁微粒化学发光法可以作为简便、快速、经济的检测方法应用于系统性红斑狼疮自身抗体的检测。%Objective: The present study aims to compare Western blot and Magnetic particle Chemiluminescence method consistency on autoimmune antibody detection of systemic lupus erythematosus. Methods: Western blot assay and Magnetic particle Chemiluminescence method were used to detect autoimmune antibody from 123 serum samples, of which 63 patients with systemic lupus erythematosus, the others from 60 healthy persons. The results of the two methods were compared by X2 test. Results: Anti-Sm antibody positive rate of Western blot and Magnetic particle Chemiluminescence method were 27.0 and 14.3 percent respectively. Anti- RNP/Sm antibody positive rates were 39.7% and 42.9% respectively. Anti- ds- DNA antibody positive rates were 41.3% and 30.2% respectively. Anti- nucleosome antibody positive rates were 31.7% and 20.6% respectively. Anti- ribosomal protein antibody positive rates were 28.6% and 11.1% respectively. The accuracy

  6. Determination of Glutamic Acid by Potassium Permanganate-glyoxal Chemiluminescence System%高锰酸钾-乙二醛化学发光体系测定谷氨酸

    Institute of Scientific and Technical Information of China (English)

    樊雪梅; 王书民

    2014-01-01

    !押建立了测定谷氨酸的化学发光新方法,确定了该方法的测定最佳条件,并研究了抑制体系的机理。在最佳条件下,谷氨酸浓度在2.0×10-8-5.0×10-5 mol·L-1范围内与相对发光强度成正比,方法的检出限为6.0×10-9 mol·L-1,对1.0×10-6 mol·L-1的谷氨酸平行测定9次,相对标准偏差为2.5%。该法用于味精产品中谷氨酸含量分析。%A novel flow injection chemiluminescence method was developed for the determination of glutamic acid, based on the inhibited effect of glutamic acid on the chemiluminescence reaction of potassium permanganate and glyoxal in the acidic medium. The optimum conditions and the possible inhibitory mechanism was also discussed. Under optimum conditions, the relative chemiluminescence intensity was 1inearly related to the concentration of glutamic acid in the range of 2.0×10-8-5.0×10-5 mol·L-1 with a detection limit of 6.0×10-9 mol·L-1, the RSD for measurement of 1.0×10-6 mol·L-1 glutamic acid (n=9) is 2.5%. This method was applied to the determination of glutamic acid in monosodium glutamate samples with satisfatory results.

  7. Determination of Ranitidine in Drug Using Flow-Injection Chemiluminescence Method%流动注射化学发光法测定药物中的雷尼替丁

    Institute of Scientific and Technical Information of China (English)

    吴芳辉; 陈乐

    2012-01-01

    A new chemiluminescence method for determination of ranitidine is proposed. The method is based on the enhancement of ranitidine on the chemiluminescence reaction among cerium sulphate and sodium sulfite in He SO4 medium. The linear relationship between the intensity of chemiluminescence and the concentration of ranitidine is in the range of 3.0×10-6~2.5× 10-4 mol/L with the detection limit of 9.8× 10-r mol/L. The relative standard deviation is 3.3% for]l measurements of 5.0× 10 a mol/L ran itidine standard solution. This method has been successfully applied to determination of ranitidine in in jections and drug capsules with satisfactory results.%在硫酸介质中,硫酸铈氧化亚硫酸钠产生微弱的发光信号,而雷尼替丁可显著增强该体系的化学发光,由此建立了流动注射化学发光技术测定雷尼替丁的新体系.实验结果表明,该方法的检出限为9.8×10-7mol/L,测定雷尼替丁的线性范围为3.0×10-6~2.5×10-4mol/L.对5.0×10-5mol/L雷尼替丁进行11次平行测定,其相对标准偏差为3.3%.将该方法用于药物胶囊中雷尼替丁的测定,结果令人满意.

  8. Determination of monoamine neurotransmitters and their metabolites in a mouse brain microdialysate by coupling high-performance liquid chromatography with gold nanoparticle-initiated chemiluminescence

    Energy Technology Data Exchange (ETDEWEB)

    Li Na; Guo Jizhao; Liu Bo; Yu Yuqi [Department of Chemistry, University of Science and Technology of China (USTC), JinZhai Road No: 96, 230026 Hefei, Anhui (China); Cui Hua, E-mail: hcui@ustc.edu.cn [Department of Chemistry, University of Science and Technology of China (USTC), JinZhai Road No: 96, 230026 Hefei, Anhui (China); Mao Lanqun; Lin Yuqing [Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences (CAS), 100080 Beijing (China)

    2009-07-10

    Our previous work showed that gold nanoparticles could trigger chemiluminescence (CL) between luminol and AgNO{sub 3}. In the present work, the effect of some biologically important reductive compounds, including monoamine neurotransmitters and their metabolites, reductive amino acids, ascorbic acid, uric acid, and glutathione, on the novel CL reaction were investigated for analytical purpose. It was found that all of them could inhibit the CL from the luminol-AgNO{sub 3}-Au colloid system. Among them, monoamine neurotransmitters and their metabolites exhibited strong inhibition effect. Taking dopamine as a model compound, the CL mechanism was studied by measuring absorption spectra during the CL reaction and the reaction kinetics via stopped-flow technique. The CL inhibition mechanism is proposed to be due to that these tested compounds competed with luminol for AgNO{sub 3} to inhibit the formation of luminol radicals and to accelerate deposition of Ag atoms on surface of gold nanoparticles, leading to a decrease in CL intensity. Based on the inhibited CL, a novel method for simultaneous determination of monoamine neurotransmitters and their metabolites was developed by coupling high-performance liquid chromatography with this CL reaction. The new method was successfully applied to determine the compounds in a mouse brain microdialysate. Compared with the reported HPLC-CL methods, the proposed method is simple, fast, and could determine more analytes. Moreover, the limits of linear ranges for NE, E, and DA using the proposed method were one order of magnitude lower than the luminol system without gold nanoparticles.

  9. A flow injection chemiluminescence method for determination of nalidixic acid based on KMnO₄-morin sensitized with CdS quantum dots.

    Science.gov (United States)

    Khataee, Alireza; Lotfi, Roya; Hasanzadeh, Aliyeh; Iranifam, Mortaza; Joo, Sang Woo

    2016-02-05

    A simple and sensitive flow injection chemiluminescence (CL) method was developed for determination of nalidixic acid by application of CdS quantum dots (QDs) in KMnO4-morin CL system in acidic medium. Optical and structural features of L-cysteine capped CdS quantum dots which were synthesized via hydrothermal approach were investigated using X-ray diffraction (XRD), scanning electron microscopy (SEM), photoluminescence (PL), and ultraviolet-visible (UV-Vis) spectroscopy. Moreover, the potential mechanism of the proposed CL method was described using the results of the kinetic curves of CL systems, the spectra of CL, PL and UV-Vis analyses. The CL intensity of the KMnO4-morin-CdS QDs system was considerably increased in the presence of nalidixic acid. Under the optimum condition, the enhanced CL intensity was linearly proportional to the concentration of nalidixic acid in the range of 0.0013 to 21.0 mg L(-1), with a detection limit of (3σ) 0.003 mg L(-1). Also, the proposed CL method was utilized for determination of nalidixic acid in environmental water samples, and commercial pharmaceutical formulation to approve its applicability. Furthermore, corona discharge ionization ion mobility spectrometry (CD-IMS) method was utilized for determination of nalidixic acid and the results of real sample analysis by two proposed methods were compared. Comparison the analytical features of these methods represented that the proposed CL method is preferable to CD-IMS method for determination of nalidixic acid due to its high sensitivity and precision.

  10. Simple and Excellent Selective Chemiluminescence-Based CS2 On-Line Detection System for Rapid Analysis of Sulfur-Containing Compounds in Complex Samples.

    Science.gov (United States)

    Zhang, Runkun; Li, Gongke; Hu, Yufei

    2015-06-02

    To study the interesting chemical reaction phenomenon can greatly contribute to the development of an innovative analytical method. In this paper, a simple CL reaction cell was constructed to study the chemiluminescence (CL) emission from the thermal oxidation of carbon disulfide (CS2). We found that the CL detection of CS2 exhibits unique characteristics of excellent selectivity and rapid response capacity. Experimental investigations together with theoretical calculation were performed to study the mechanism behind the CL reaction. The results revealed that the main luminous intermediates generated during the thermal degradation of CS2 are SO2* and CO2*. Significantly, this CL emission phenomenon has a wide application due to many sulfur-containing compounds that can convert to CS2 under special conditions. On the basis of this scheme, a CS2-generating and detection system was developed for rapid measurement of CS2 or other compounds that can convert to CS2. The usefulness of the system was demonstrated by measuring dithiocarbamate (DTC) pesticides (selected mancozeb as a representative analyte) based on the evolution of CS2 in spiked agricultural products. Results showed that the system allows online and large volume detection of CS2 under nonequilibrium condition, which greatly reduces the analytical time. The concentrations of mancozeb in the spiked samples were well-quantified with satisfied recoveries of 76.9-97.3%. The system not only addresses the urgent need for rapid in-field screening of DTC residues in foodstuffs but also opens a new opportunity for the fast, convenient, and cost-effective detection of CS2 and some other sulfur-containing compounds in complex samples.

  11. Enhancement of protein kinase C activity and chemiluminescence intensity in mitochondria isolated from the kidney cortex of rats treated with cephaloridine.

    Science.gov (United States)

    Kohda, Yuka; Gemba, Munekazu

    2002-08-01

    The development of nephrotoxicity induced by cephaloridine (CER) has been reported to be due to reactive oxygen species (ROS). Protein kinase C (PKC) has been suggested to modulate the generation of ROS. We investigated the possible participation of ROS generation assessed by chemiluminescence (CL) and PKC activity in rat kidney cortical mitochondria in the development of CER-induced nephrotoxicity. We first evaluated the magnitude of the nephrotoxic damage caused by CER in rats. The plasma parameters and ultrastructural morphology changes were increased markedly 24hr after the treatment of rats with CER. We demonstrated that the treatment of rats with CER clearly evoked not only enhancement of Cypridina luciferin analog (CLA)-dependent CL intensity, but also the activation of PKC in mitochondria isolated from the kidney cortex of rats 1.5 and 3.5 hr after injection of the drug. These changes were detected in advance of those observed in plasma and by electron microscopy. The increase in CLA-dependent CL intensity detected in the kidney cortical mitochondria 1.5 and 3.5 hr after injection of CER was inhibited completely by the addition of superoxide dismutase, suggesting the generation of superoxide anion in these mitochondria during the early stages of CER-induced nephrotoxicity. These results suggest that the activation of PKC and the enhancement of superoxide anion generation in kidney cortical mitochondria precede the increases in plasma parameters and the electron micrographic changes indicative of renal dysfunction in rats treated with CER. Additionally, they suggest a possible relationship between PKC activation in mitochondria and free radical-induced CER nephrotoxicity in rats.

  12. Electrogenerated chemiluminescence biosensing for the detection of prostate PC-3 cancer cells incorporating antibody as capture probe and ruthenium complex-labelled wheat germ agglutinin as signal probe

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Haiying [Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062 (China); Department of Chemistry, Yuncheng University, Yuncheng 044300 (China); Li, Zhejian; Shan, Meng; Li, Congcong; Qi, Honglan; Gao, Qiang [Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062 (China); Wang, Jinyi [College of Science and College of Veterinary Medicine, Northwest A& F University, Yangling 712100 (China); Zhang, Chengxiao, E-mail: cxzhang@snnu.edu.cn [Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062 (China)

    2015-03-10

    Highlights: • A novel biosensor was developed for the detection of prostate cancer cells. • The selectivity of the biosensor was improved using antibody as capture probe. • The biosensor showed the low extremely detection limit of 2.6 × 10{sup 2} cells mL{sup −1}. • The ruthenium complex-labelled WGA can be transported in the cell vesicles. - Abstract: A highly selective and sensitive electrogenerated chemiluminescence (ECL) biosensor for the detection of prostate PC-3 cancer cells was designed using a prostate specific antibody as a capture probe and ruthenium complex-labelled wheat germ agglutinin as a signal probe. The ECL biosensor was fabricated by covalently immobilising the capture probe on a graphene oxide-coated glassy carbon electrode. Target PC-3 cells were selectively captured on the surface of the biosensor, and then, the signal probe was bound with the captured PC-3 cells to form a sandwich. In the presence of tripropylamine, the ECL intensity of the sandwich biosensor was logarithmically directly proportion to the concentration of PC-3 cells over a range from 7.0 × 10{sup 2} to 3.0 × 10{sup 4} cells mL{sup −1}, with a detection limit of 2.6 × 10{sup 2} cells mL{sup −1}. The ECL biosensor was also applied to detect prostate specific antigen with a detection limit of 0.1 ng mL{sup −1}. The high selectivity of the biosensor was demonstrated in comparison with that of a lectin-based biosensor. The strategy developed in this study may be a promising approach and could be extended to the design of ECL biosensors for highly sensitive and selective detection of other cancer-related cells or cancer biomarkers using different probes.

  13. Oxidation state-differentiated measurement of aqueous inorganic arsenic by continuous flow electrochemical arsine generation coupled to gas-phase chemiluminescence detection.

    Science.gov (United States)

    Sengupta, Mrinal K; Dasgupta, Purnendu K

    2011-12-15

    The electrochemical reduction of inorganic As on a graphite cathode depends on the current density. We observed that while only inorganic As(III) is reduced to AsH(3) at low current densities, at high current densities both forms of inorganic As are reduced. We describe a unique electrochemical reactor in which the cylindrical anode compartment is isolated from the outer concentric cathode compartment by a Nafion tube in which a hole is deliberately made and the entire anode compartment is inside the cylindrical cavity of a small volume (∼115 μL) cathode chamber. The evolved arsine is then quantitated by gas-phase chemiluminescence (GPCL) reaction with ozone; the latter is generated from oxygen formed during electrolysis. For the dimensions used, inorganic As(III) can be selectively determined at a current of 0.1 A while total inorganic As (both As(III) and As(V)) respond equally at an applied electrolysis current at 0.85 A, without any sample treatment. For a 1-mL sample, the system provides a limit of detection (LOD, S/N = 3) of 0.09 μg/L for total As (i = 0.85 A) and an LOD of 0.76 μg/L for As(III) (i = 0.10 A); As(V) is obtained by difference. Comparison of ICP-MS results for total As in groundwater samples that span a large range of concentration and total inorganic As determined by the present method showed a high correlation (r(2) = 0.9975) and a near unity slope. The basic electrochemical arsine generation technique and current-differentiated oxidation state speciation should be applicable as the front end to many other arsenic measurements techniques, including atomic spectrometry.

  14. Development of a multiplexed chemiluminescent immunochemical imaging technique for the simultaneous localization of different proteins in painting micro cross-sections.

    Science.gov (United States)

    Sciutto, Giorgia; Dolci, Luisa Stella; Buragina, Angela; Prati, Silvia; Guardigli, Massimo; Mazzeo, Rocco; Roda, Aldo

    2011-03-01

    The identification and localization of organic components in the complex stratigraphy of paintings play a crucial role in studies of painting techniques and authentication, restoration, and conservation of artworks. Much scientific effort has been expended for the development of analytical approaches suitable for the investigation and characterization of organic substances, allowing high sensitivity, specificity, and spatial resolution. Proteins (e.g., ovalbumin, casein, and collagen from different animal sources) are one of the classes of organic substances most widely used as painting materials. The analytical techniques commonly used for their analysis (micro Fourier transform infrared spectroscopy, chromatographic techniques, and proteomic approaches) have limits related to the lack of specificity or to the absence of information concerning the stratigraphic localization of the detected proteins. Immunological techniques are a promising alternative approach for the characterization of proteins in artworks. Thanks to the high specificity of antigen-antibody reactions, these techniques are widely used for the analysis of proteins in bioanalytical and clinical chemistry and recently they have been successfully applied in the field of science for conservation of cultural heritage. The present research aimed to develop an ultrasensitive chemiluminescent immunochemical procedure for the simultaneous localization of ovalbumin and bovine casein (two common proteins found in binding media or varnishes of artistic and archaeological samples) in resin-embedded painting micro cross-sections. The possibility of performing the simultaneous identification of different proteins in painting cross-sections is of particular relevance in the field of cultural heritage because samples are often small and available in a limited number; therefore, the maximum amount of information must be obtained from each of them.

  15. Quimioluminiscencia como método de screening de cáncer oral Chemiluminescence as a screening method in oral cancer

    Directory of Open Access Journals (Sweden)

    A. Carrera Torres

    2011-12-01

    Full Text Available El cáncer oral es un problema de salud con el que nos enfrentamos todos los días en nuestras consultas. Los dentistas formamos la primera línea de defensa ante una patología que no ha presentado una mejora de los índices de mortalidad y morbilidad en las últimas décadas. La principal arma con la que contamos es la del diagnóstico precoz de la enfermedad, y esto es mucho más complicado de lo que parece. Para ayudar a los dentistas en la visualización de lesiones de precáncer o cáncer temprano han aparecido algunas técnicas o dispositivos de screening de cáncer oral basadas en la interacción de los tejidos orales con la luz. La técnica más extendida y estudiada es la basada en la quimioluminiscencia, intentaremos poner en claro algunos aspectos analizando los estudios que hay en la literatura actual.Oral cancer is a health problem with which we face every day in our consultations. Dentists form the first line of defense against a disease that has not produced an improvement in mortality and morbidity in recent decades. The main weapon with which we have is the early diagnosis of the disease, and this is much more complicated than it seems. To assist dentists in the visualization of pre-cancer lesions or early cancer have appeared some techniques and devices for oral cancer screening based on interaction of the oral tissues with light. The most widely used and studied technique is based on the chemiluminescence. Above it has been much talk and try to clarify some aspects of the studies that are analyzing the current literature.

  16. Evaluation of a chemiluminescence method for measuring alkaline phosphatase activity in whole milk of multiple species and bovine dairy drinks: interlaboratory study.

    Science.gov (United States)

    Salter, Robert S; Fitchen, John

    2006-01-01

    Alkaline phosphatase (ALP) is a ubiquitous enzyme in milk with time-temperature destruction similar to that of certain pathogens destroyed in pasteurization. Measurement of ALP to indicate proper pasteurization is a common practice. Recently the public health level for ALP was decreased to 350 mU/L, a level below the sensitivity of older colorimetric ALP methods. This study was conducted within the structure of the International Dairy Federation and the International Organization for Standardization to evaluate the reproducibility of the chemiluminescence method (Charm PasLite) for ALP at 50, 100, 350, and 500 mU/L in whole milk of multiple species to meet new regulations in the United States and proposed regulations in the European Union (EU). Fifteen laboratories from 8 countries evaluated bovine, goat, sheep, and buffalo milk, bovine skim milk, 20% fat cream, and 2% fat chocolate milk. At ALP levels of 350 and 500 mU/L, the average relative standard deviation for repeatability (RSDr) was 7.5%, and the average relative standard deviation of reproducibility was (RSDR) 15%. For ALP at 100 and 50 mU/L, the average RSDr values were 10.5 and 12.6%, respectively, and the average RSDR values were 18 and 25%, respectively. The limit of detection was 20 mU/L. Results are comparable to those obtained with other enzymatic photo-activated system methods such as the fluorometric method. Results indicate that the method is suitable for measuring ALP in the milk of multiple species and in dairy drinks at U.S. and proposed EU levels.

  17. The use of imidazolium ionic liquid/copper complex as novel and green catalyst for chemiluminescent detection of folic acid by Mn-doped ZnS nanocrystals

    Science.gov (United States)

    Azizi, Seyed Naser; Shakeri, Parmis; Chaichi, Mohammad Javad; Bekhradnia, Ahmadreza; Taghavi, Mehdi; Ghaemy, Mousa

    2014-03-01

    A novel chemiluminescence (CL) method using water-soluble Mn-doped ZnS quantum dots (QDs) as CL emitter is proposed for the chemiluminometric determination of folic acid in pharmaceutical formulation. Water-soluble Mn-doped ZnS QDs were synthesized by using L-cysteine as stabilizer in aqueous solutions. The nanoparticles were structurally and optically characterized by X-ray powder diffraction (XRD), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), UV-Vis absorption spectroscopy and photoluminescence (PL) emission spectroscopy. The CL of ZnS QDs induced by directly chemical oxidation and its ionic liquid-sensitized effect in aqueous solution were then investigated. It was found that oxidants, especially hydrogen peroxide, could directly oxidize ZnS QDs to produce weak CL emission in basic conditions. In the presence of 1,3-dipropylimidazolium bromide/copper a drastic light emission enhancement is observed, related to a strong interaction between Cu2+ and the imidazolium ring. Therefore, a new CL analysis system was developed for the determination of folic acid. Under the optimum conditions, there is a good linear relationship between the relative CL intensity and the concentration of folic acid in the range of 1 × 10-9-1 × 10-6 M of folic acid with a correlation coefficient (R2) of 0.9991. The limit of detection of this system was found to be 1 × 10-10 M. This method is not only simple, sensitive and low cost, but also reliable for practical applications.

  18. Universal chemiluminescence flow-through device based on directed self-assembly of solid-state organic chromophores on layered double hydroxide matrix.

    Science.gov (United States)

    Wang, Zhihua; Teng, Xu; Lu, Chao

    2013-02-19

    In this work, a universal chemiluminescence (CL) flow-through device suitable for various CL resonance energy transfer (CRET) systems has been successfully fabricated. Highly efficient CRET in solid-state photoactive organic molecules can be achieved by assembling them on the surface of layered double hydroxides (LDHs). We attribute these observations to the suppression of the intermolecular π-π stacking interactions among aromatic rings and the improvement of molecular orientation and planarity in the LDH matrix, enabling a remarkable increase in fluorescence lifetime and quantum yield of organic molecules. Under optimal conditions, using peroxynitrous acid-fluorescein dianion (FLUD) as a model CRET system, trace FLUD (10 μM) was assembled on the surface of LDHs. Peroxynitrous acid/nitrite could be assayed in the range of 1.0-500 μM, and the detection limit for peroxynitrous acid/nitrite (S/N = 3) was 0.6 μM. This CL flow-through device exhibited operational stability, high reproducibility, and long lifetime. While LDHs were immobilized in a flow-through device in the absence of FLUD, the detection limit for peroxynitrous acid/nitrite was 100 μM. On the other hand, FLUD at the same concentration can not enhance the CL intensity of peroxynitrous acid system. This fabricated CL flow-through column has been successfully applied to determine nitrite in sausage samples with recoveries of 98-102%. These satisfactory results demonstrated that our studies pave a novel way toward flow-through column-based CRET using solid-state organic molecules as acceptors for signal amplification.

  19. Reactive oxygen species scavenging activities in a chemiluminescence model and neuroprotection in rat pheochromocytoma cells by astaxanthin, beta-carotene, and canthaxanthin.

    Science.gov (United States)

    Chang, Chi-Sen; Chang, Chia-Lin; Lai, Guia-Hung

    2013-08-01

    The objective of this study was to determine chemiluminescence (CL) antioxidant activities and neuroprotective effects of astaxanthin, beta-carotene (β-carotene), and canthaxanthin on undifferentiated rat pheochromocytoma (PC12) cells. We performed three CL antioxidant assays, and the three carotenoids showed varying degrees of antioxidant activity, with astaxanthin exhibiting the highest antioxidant activity than the other two samples. Results of a pyrogallol-luminol assay revealed β-carotene to have higher antioxidant activity than canthaxanthin, whereas cupric sulfate-Phen-Vc-hydrogen peroxide (H₂O₂) assay showed canthaxanthin to have higher antioxidant activity than β-carotene. Luminol-H₂O₂ assay showed the antioxidant activity series as canthaxanthin > β-carotene at 62.5-1000 μg/mL and β-carotene > canthaxanthin at 1000-4000 μg/mL. Astaxanthin exhibited partial neuroprotective activity against H₂O₂ and the strongest neuroprotective activity against amyloid beta-peptide(25-35) [(Aβ)(25-35)]-induced undifferentiated PC12 cell deaths at 0.5-5.0 μM. Canthaxanthin showed partial neuroprotective activity in Aβ(25-35)-induced undifferentiated PC12 cell deaths at 1.0-5.0 μM. Astaxanthin protected undifferentiated PC12 cells from the damaging effects of H₂O₂ and Aβ(25-35) by the following ways: (1) scavenging superoxide anion radicals, hydroxyl radicals, and H₂O₂; (2) securing cell viability; (3) suppressing the production of reactive oxygen species; and (4) eliminating calcium ion influx. Our results conclusively show that astaxanthin has the merit as a potential neuron protectant.

  20. Determination of phenols by flow injection and liquid chromatography with on-line quinine-sensitized photo-oxidation and quenched luminol chemiluminescence detection

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Wei; Danielson, Neil D

    2003-10-01

    An on-line quinine-sensitized photo-oxidation with quenched chemiluminescence (CL) detection method is developed for phenols using flow injection (FI) and liquid chromatography (LC). This detection method is based on the decrease of light emission from the luminol CL reaction due to the photo-oxidation of phenols that scavenge the photogenerated reactive oxygen species (e.g. singlet oxygen ({sup 1}O{sub 2}) and superoxide (O{sub 2}{center_dot}{sup -})). On-line photo-oxidation is achieved using a coil photo-reactor made from fluoroethylene-propylene copolymer tubing (3048 mmx0.25 mm i.d.) coiled around a mercury UV lamp. A buffer of pH 7 and a concentration of 350 {mu}M for quinine sulfate are determined optimum for the sensitized photo-oxidation. Using a carrier system flow rate of 60 {mu}l/min, calibration curves taken by FI for 10 phenolic compounds in aqueous solutions showed this decreasing sensitivity order: 4-chlorophenol, phenol, 4-nitrophenol, 3-hydroxy-L-kynurenine, 2-nitrophenol, salicylate, 3-nitrophenol, catechol, 2,4-dinitrophenol, and 2,4-dichlorophenol. This detection method using two tandem coil photo-reactors is also applied for the LC separation of phenol, 4-nitrophenol and 4-chlorophenol on an octadecyl (C18) silica LC column using acetonitrile-H{sub 2}O (40:60, v/v) as a mobile phase. The quenched CL detection limits (about 1 {mu}M or 20 pmol) for phenol and 4-chlorophenol are comparable to those for UV detection at 254 nm. Some selectivity in the quenched CL detection is evident by no interference in the FI phenol response even when benzaldehyde and phenethanol concentrations are 8 and 15 times that of phenol.

  1. Multifunctional reduced graphene oxide trigged chemiluminescence resonance energy transfer: Novel signal amplification strategy for photoelectrochemical immunoassay of squamous cell carcinoma antigen.

    Science.gov (United States)

    Zhang, Yan; Sun, Guoqiang; Yang, Hongmei; Yu, Jinghua; Yan, Mei; Song, Xianrang

    2016-05-15

    Herein, a photoelectrochemical (PEC) immunoassay is constructed for squamous cell carcinoma antigen (SCCA) detection using zinc oxide nanoflower-bismuth sulfide (Bi2S3) composites as photoactive materials and reduced graphene oxide (rGO) as signal labels. Horseradish peroxidase is used to block sites against nonspecific binding, and then participated in luminol-based chemiluminescence (CL) system. The induced CL emission is acted as an inner light source to excite photoactive materials, simplifying the instrument. A novel signal amplification strategy is stem from rGO because of the rGO acts as an energy acceptor, while luminol serves as a donor to rGO, triggering the CL resonance energy transfer phenomenon between luminol and rGO. Thus, the efficient CL emission to photoactive materials decreases. Furthermore, the signal amplification caused by rGO labeled signal antibodies is related to photogenerated electron-hole pairs: perfect matching of energy levels between rGO and Bi2S3 makes rGO a sink to capture photogenerated electrons from Bi2S3; the increased steric hindrance hinders the electron donor to the surface of Bi2S3 for reaction with the photogenerated holes. On the basis of the novel signal amplification strategy, the proposed immunosensor exhibits excellent analytical performance for PEC detection of SCCA, ranging from 0.8 pg mL(-1) to 80 ng mL(-1) with a low detection limit of 0.21 pg mL(-1). Meanwhile, the designed signal amplification strategy provides a general format for future development of PEC assays.

  2. Combination of capillary micellar liquid chromatography with on-chip microfluidic chemiluminescence detection for direct analysis of buspirone in human plasma.

    Science.gov (United States)

    Al Lawati, Haider A J; Kadavilpparampu, Afsal Mohammed; Suliman, FakhrEldin O

    2014-09-01

    Microfluidic based chemiluminescence (CL) detector having novel channel design for enhanced mixing has been developed and investigated in terms of its applicability with micellar mode of liquid chromatography (MLC). The newly developed detector was found to be highly sensitive and an alternative detection technique to combine with capillary MLC. This combination was successfully employed for direct detection of a model analyte using Ru(III)-peroxydisulphate CL system. The selected analyte, buspirone hydrochloride (BUS), was detected selectively at therapeutic concentration levels in human plasma without any sample pretreatment. By incorporating eight flow split units within the spiral channel of microfluidic chip, an enhancement of 140% in CL emission was observed. We also evaluated the effect of non- ionic surfactant, Brij-35, which used as mobile phase modifier in MLC, on CL emission. The CL signal was improved by 52% compared to aqueous-organic mobile phase combinations. Various parameters influencing the micellar chromatographic performance and the CL emission were optimized. This allowed highly sensitive analysis of BUS with limit of detection (LOD) of 0.27 ng mL(-1) (3σ/s) and limit of quantification (LOQ) of 0.89 ng mL(-1) (10σ/s). The analyte recovery from human plasma at three different concentration level ranges from 88% to 96% (RSD 1.9-5.3%). The direct analysis of BUS in human plasma was achieved within 6 min. Therefore, combining microfluidic CL detection with micellar mode of separation is an efficient, cost-effective and highly sensitive technique that can utilize MLC in its full capacity for various bioanalytical procedures.

  3. Chemiluminescence determination of cefotaxime sodium with flow-injection analysis of cerium (IV)-rhodamine 6G system and its application to the binding study of cefotaxime sodium to protein with on-line microdialysis sampling

    Science.gov (United States)

    Chen, Dinglong; Wang, Huiying; Zhang, Zhujun; Ci, Lijie; Zhang, Xuehong

    2011-01-01

    A simple, sensitive and rapid flow-injection chemiluminescence (CL) method has been developed for the determination of cefotaxime sodium based on the chemiluminescence reaction of cefotaxime sodium with ceric sulfate and rhodamine 6G in nitric acid solution. The concentration of cefotaxime sodium was proportional with the CL intensity in the range of 4 × 10 -8 - 8 × 10 -6 mol L -1. The detection limit (signal-to-noise ratio = 3) was 1 × 10 -8 mol L -1. Coupled to the technique of on-line microdialysis sampling, this method was successfully applied to study cefotaxime sodium-protein interaction. The drug and protein were mixed in different molar ratios in Ringer's solution, pH 7.4, and incubated at 37 °C in a water bath. The microdialysis probe was utilized to sample the mixed solution at a perfusion rate of 5 μL min -1 and the recovery of cefotaxime sodium under experimental condition was 16.2%. The data obtained by the present Microdialysis-Flow Injection Analysis-CL method was analyzed with the Scatchard analysis and Klotz plot. The estimated association constant ( K) and the number of the binding sites ( n) on one of BSA molecule were 5.94 × 10 4 M -1 and 1.29 (Klotz equation), respectively.

  4. Rapid Determination of Sodium Saccharin by FI-Chemiluminescence%流动注射-化学发光法快速测定糖精钠

    Institute of Scientific and Technical Information of China (English)

    高向阳; 魏姜勉; 王珊; 尹中瑞

    2011-01-01

    FI-Chemiluminescence determination of sodium saccharin based on the CL reaction between luminol and H2O2 was reported.In an acid medium,the solution of sodium saccharin was reacted with K2Cr2O7 to reduce Cr(Ⅵ) quantitatively to Cr(Ⅲ) and the CL intensity of the reaction system was remarkably enhanced by the Cr(Ⅲ) formed.The content of Cr(Ⅲ) was then determined and converted into contents of sodium saccharin.Linear relationship between values of increase in CL intensity and concentration of sodium saccharin was obtained in the range of 5.0×10-7-1.0×10-5mol·L-1.Detection limit(3s) was found to be 1.08×10-11mol·L-1.Recovery was tested by addition of standard solutions at 3 concentration levels to sample solutions,values of recovery obtained were in the range of 95.6%-105.8% with RSD(n=12) of 2.9%.%提出了基于鲁米诺与过氧化氢化学发光反应的流动注射-化学发光法快速测定糖精钠的方法。在酸性介质中,糖精钠与重铬酸钾反应定量生成铬(Ⅲ),所得的铬(Ⅲ)可显著增加上述化学发光反应的化学发光强度,且其强度的增加值与糖精钠浓度在5.0×10-7~1.0×10-5 mol.L-1范围内呈线性关系,方法的检出限(3s)为1.08×10-11 mol.L-1。在试样溶液中分别加入3个浓度水平的糖精钠标准溶液,按方法测定求得其回收率在95.6%~105.8%之间,相对标准偏差(n=12)为2.9%。

  5. FI-Inhibitory Chemiluminescence Determination of Carbofuran%流动注射-抑制化学发光法测定克百威

    Institute of Scientific and Technical Information of China (English)

    贾风燕; 刘振波; 王荣; 刘永明

    2011-01-01

    Based on the inhibitory effect of carbofuran on the CL-reaction system of luminol-H2O2-sodium copper chlorophyll in an alkaline medium, a method for the determination of carbofuran by FI-inhibitory chemiluminescence (CL) was proposed. It was found that a stable complex was formed by the reaction of sodium copper chlorophyll with carbofuran in the mole ratio of 1 to 1, the process of quenching of fluorescence of carbofuran was proved to be a process of static quenching. Equilibrium constant (K0) and combination distance (r) the complex were determined and values obtained were 3. 41 × 105L · mol-1 and 0. 39 nm respectively. Linear relationship was found between values of CL intensity and mass concentration of carbofuran in the range of 0. 08 to 2. 0 mg · L-1,and detection limit (30/k) of the method found was 0. 03 mg · L-1 The proposed method was used in analysis of carbofuran in secticide samples, giving values of average recovery of 101.5%.%基于在碱性介质中,克百威抑制鲁米诺-过氧化氢-叶绿素铜钠体系的化学发光,提出了流动注射-抑制化学发光法测定克百威含量的方法.试验结果表明:叶绿素铜钠对克百威荧光猝灭过程是静态猝灭过程,叶绿素铜钠与克百威结合形成物质的量比为1比1的稳定配合物,平衡常数(K0)为3.41×105L·mol-1(25℃),结合距离(r)为0.39nm.克百威质量浓度在0.08~2.00 mg·L-1范围内与其发光强度呈线性关系,方法检出限(3σ/k)为0.03 mg·L-1.此法用于克百威杀虫剂样品的分析,测得方法的平均加标回收率为101.5%.

  6. The performance verification of Abbott i2000 chemiluminescence detection TPSA%雅培i2000化学发光检测TPSA的性能验证

    Institute of Scientific and Technical Information of China (English)

    陈刚; 张彬; 王晶; 邓蔷; 罗立梅; 苏建蓉

    2016-01-01

    目的:按IS015189要求验证雅培i2000化学发光检测TPSA分析性能。方法:对总前列腺特异性抗原(TPSA)精密度、正确度、线性范围、自动稀释倍数、功能灵敏度和参考区间进行验证。结果:批内或批间变异系数(CV)不仅小于卫生部室间质评标准精密度参数,也小于厂家提供准精密度参数;对配套定值血清测定值与靶值之间的偏差小于允许偏差;线性范围与厂家提供范围一致;自动稀释倍数偏差符合临床检测要求;功能灵敏度和参考区间验证通过。结论:检测系统的基本性能达到临床化学检验领域的要求,也符合肿瘤标志物临床应用指导原则的要求。可将经过评价的检测系统用于常规工作。%Objective To verify the performance of Abbott i2000 chemiluminescence in the detection of TPSA according to the requirement of IS015189. Methods The precision, accuracy, automatic dilution, linear range, functional sensitivity and reference interval of total prostate specific antigen (TPSA) were validated. Results The intra or inter assay coefficient of variation (CV) is not only less than standard precision parameters on quality assessment of health department, but also less than manufacturers provided accurate density parameter;The deviation value between serum measured value and the target value is less than the allowable devia-tion;Linear range is consistent with that provided by the manufacturers;Automatic dilution bias is also consistent with clinical testing requirements;functional sensitivity and reference interval validation passed. Conclusion The basic performance of the detection sys-tem can meet the requirements of clinical chemistry examination, and also meet the requirements of clinical application of tumor markers. The evaluation system can be used for routine work.

  7. Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork

    Energy Technology Data Exchange (ETDEWEB)

    Dong Jiexian; Li Zhenfeng; Lei Hongtao; Sun Yuanming [Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642 (China); Ducancel, Frederic [CEA, iBiTec-S, Service de Pharmacologie et d' Immnoanalyse (SPI), CEA Saclay, F-91191 Gif sur Yvette (France); Xu Zhenlin [Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642 (China); Boulain, Jean-Claude [CEA, iBiTec-S, Service de Pharmacologie et d' Immnoanalyse (SPI), CEA Saclay, F-91191 Gif sur Yvette (France); Yang Jinyi; Shen Yudong [Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642 (China); Wang Hong, E-mail: gzwhongd@63.com [Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642 (China)

    2012-07-29

    Graphical abstract: Detection model of dc-CLEIA based on anti-RAC scFv-AP fusion protein. Highlights: Black-Right-Pointing-Pointer The scFv-AP fusion protein against ractopamine (RAC) was produced. Black-Right-Pointing-Pointer A dc-CLEIA for RAC was developed based on the purified scFv-AP fusion protein. Black-Right-Pointing-Pointer The sensitivity of dc-CLEIA was 10 times as sensitive as dc-ELISA for RAC. Black-Right-Pointing-Pointer Recovery tests from pork samples were studied. Black-Right-Pointing-Pointer Good accuracy was obtained. - Abstract: A rapid, sensitive chemiluminescent enzyme immunoassay (CLEIA) for ractopamine (RAC) based on a single-chain variable fragment (scFv)-alkaline phosphatase (AP) fusion protein was developed. The scFv gene was prepared by cloning the heavy- and light-chain variable region genes (V{sub H} and V{sub L}) from hybridoma cell line AC2, which secretes antibodies against RAC, and assembling V{sub H} and V{sub L} genes with a linker by means of splicing overlap extension polymerase chain reaction. The resulting scFv gene was inserted into the expression vector pLIP6/GN containing AP to produce the fusion protein in Escherichia coli strain BL21. The purified scFv-AP fusion protein was used to develop a direct competitive CLEIA (dcCLEIA) protocol for detection of RAC. The average concentration required for 50% inhibition of binding and the limit of detection of the assay were 0.25 {+-} 0.03 and 0.02 {+-} 0.004 ng mL{sup -1}, respectively, and the linear response range extended from 0.05 to 1.45 ng mL{sup -1}. The assay was 10 times as sensitive as the corresponding enzyme-linked immunosorbent assay based on the same fusion protein. Cross-reactivity studies showed that the fusion protein did not cross react with RAC analogs. DcCLEIA was used to analyze RAC spiked pork samples, and the validation was confirmed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS). The results showed a good correlation between

  8. Application of a newly developed high-sensitivity HBsAg chemiluminescent enzyme immunoassay for hepatitis B patients with HBsAg seroclearance.

    Science.gov (United States)

    Shinkai, Noboru; Matsuura, Kentaro; Sugauchi, Fuminaka; Watanabe, Tsunamasa; Murakami, Shuko; Iio, Etsuko; Ogawa, Shintaro; Nojiri, Shunsuke; Joh, Takashi; Tanaka, Yasuhito

    2013-11-01

    We modified and automated a highly sensitive chemiluminescent enzyme immunoassay (CLEIA) for surface antigen (HBsAg) detection using a combination of monoclonal antibodies, each for a specific epitope of HBsAg, and by improving an earlier conjugation technique. Of 471 hepatitis B virus (HBV) carriers seen in our hospital between 2009 and 2012, 26 were HBsAg seronegative as determined by the Abbott Architect assay. The Lumipulse HBsAg-HQ assay was used to recheck those 26 patients who demonstrated seroclearance by the Abbott Architect assay. The performance of the Lumipulse HBsAg-HQ assay was compared with that of a quantitative HBsAg detection system (Abbott Architect) and the Roche Cobas TaqMan HBV DNA assay (CTM) (lower limit of detection, 2.1 log copies/ml) using blood serum samples from patients who were determined to be HBsAg seronegative by the Abbott Architect assay. Ten patients had spontaneous HBsAg loss. Of 8 patients treated with nucleotide analogues (NAs), two were HBsAg seronegative after stopping lamivudine therapy and 6 were HBsAg seronegative during entecavir therapy. Eight acute hepatitis B (AH) patients became HBsAg seronegative. Of the 26 patients, 16 were HBsAg positive by the Lumipulse HBsAg-HQ assay but negative by the Abbott Architect assay. The differences between the two assays in terms of detectable HBsAg persisted over the long term in the spontaneous loss group (median, 10 months), the NA-treated group (2.5 months), and the AH group (0.5 months). In 9 patients, the Lumipulse HBsAg-HQ assay detected HBsAg when HBV DNA was negative by the CTM assay. HBsAg was also detected by the Lumipulse HBsAg-HQ assay in 4 patients with an anti-HBs concentration of >10 mIU/ml, 3 of whom had no HBsAg escape mutations. The automatic, highly sensitive HBsAg CLEIA Lumipulse HBsAg-HQ is a convenient and precise assay for HBV monitoring.

  9. Recent Advance in Chemiluminescence Assay and Its Biochemical Applications%化学发光在生化分析中的应用研究进展

    Institute of Scientific and Technical Information of China (English)

    刘萌; 王子月; 张春阳

    2016-01-01

    化学发光分析是利用化学发光反应的发光现象,对化学发光物质由激发态跃迁回基态时发出的光信号进行测量的一种分析方法。化学发光分析具有无需外来光源、灵敏度高、操作方便、分析快速以及易于实现自动化等优点,可与其它分析技术联用,在临床检验、药物分析和环境监测等领域具有广泛应用。近年来,纳米材料、生物芯片及微流控技术的引入促进了化学发光分析技术的发展。本文综述了化学发光分析与高效液相色谱、毛细管电泳、量子点、微流控芯片和微阵列、以及滚环扩增、等温指数扩增和两级等温扩增联用技术的发展,介绍了化学发光分析技术在DNA、生物小分子、生物酶、蛋白质和金属离子检测中的应用研究进展,并展望了其发展趋势。%Chemiluminescence ( CL) assay is able to measure the optical signal emitted from the CL reagents as a result of the transition from the excited state back to the ground state. CL assay has significant advantages such as no external light source, high sensitivity, convenient operation, rapid analysis and easy automation, and has wide applications in clinical test, drug analysis and environmental monitoring. Recently, the introduction of nanomaterials, biochip and microfluidic techniques promote the development of CL assay. In this review, we summarize the recent progress in CL assay with the integration of high performance liquid chromatography, capillary electrophoresis, quantum dots, microfluidic chips, microarrays, rolling circle amplification, isothermal exponential amplification, and two-stage isothermal amplification for the detection of DNA, small biological molecules, enzymes, proteins and metal ions. We also give a summary of its future directions and highlight its potential applications.

  10. 有机磷农药多残留检测化学发光标记物的合成及鉴定%Synthesis and identification of chemiluminescent markers used in the multi-residue determination of organophosphorus pesticides

    Institute of Scientific and Technical Information of China (English)

    刘涛; 孟超; 姚鑫; 李景娜; 程燕; 刘晓宇

    2014-01-01

    Objective Chemiluminescent markers were synthesized using O,O-diethylthiophosphoryl chloride and luminol for the multi-residue determination of organophosphorus pesticides. Methods Luminol and O, O-diethyl phosphoryl chloride were added to dichloromethane under ice-cooling with triethylamine as acid binding agent. After 8 h, the organic layer was separated and purified by column chromatography. Results The product was the chemiluminescent markers after indentation using UV and IR. It had a strong luminescent ability on the BPCL, and the maximum luminous intensity reached 9000 mV. Conclusion The chemilumi-nescent markers synthesized by this method had a strong luminescence capability, and it could be used in the multi-residue determination of organophosphorus pesticides.%目的:以 O,O-二乙基硫代磷酰氯和鲁米诺为原料,合成一种新型的化学发光标记物,用于后续的二乙氧基类有机磷农药多残留化学发光免疫法检测。方法取鲁米诺和O,O-二乙基硫代磷酰氯溶于二氯甲烷中,以三乙胺作为缚酸剂,冰浴下反应8h后分出有机层,产物经柱层析纯化,即得到化学发光标记物。结果产物经紫外、红外鉴定,实验结果表明反应产物即为所需的化学发光标记物,产物在BPCL上进行发光特性测定,表现出较强的发光能力,最大发光强度达到9000 mV。结论本方法合成的化学发光标记物具有较强的发光能力,能用于后续的二乙氧基类有机磷农药多残留的化学发光免疫检测。

  11. Sensitive Electrogenerated Chemiluminescence Sensor for Determination of Heroin%联吡啶钌电化学发光传感器测定海洛因

    Institute of Scientific and Technical Information of China (English)

    孙波; 漆红兰; 凌晨; 张翠芹

    2009-01-01

    An electrogenerated chemiluminescence ( ECL) sensor was fabricated by employing ruthenium complex polymer as ECL signal and ionic liquid as binding reagent and a sensitive ECL method for the determination of heroin was developed. The ECL sensor was prepared by thoroughly mixing ruthenium complex polymer,graphite powder and ionic liquid,which shows good electrochemical and ECL behaviors. The detection limit for tripropylamine (TPA) was one order of magnitude lower than that observed at the paraffin modified carbon paste electrode. ECL intensity of the fabricated sensor was greatly enhanced in the presence of heroin and a sensitive ECL method was proposed for the determination of heroin based on the enhancement ECL. The ECL intensity was linear with the concentration of heroin in the range from 2. 0 ×10~(-9) mol/L to 2. 0×10~(-5) mol/L and the detection limit was 8×10~(-10) mol/L. The ECL sensor exhibited a long-term stability,fine reproducibility with relative standard deviation less than 5% for 5. 0×10~(-9) mol/L heroin in 60 continuous determinations. The developed method allows the detection of heroin in a serum sample with recovery in 94% -101%.%利用离子液体为粘合剂制作碳糊电极,采用高分子聚合法,合成包埋有Ru(bpy)_2(dcbpy)~(2+)的高分子聚合物,将钌聚合物掺杂于离子液体碳糊电极中,制作电化学发光传感器.结果表明,此传感器具有很好的电化学发光特性,与用石蜡油为粘合剂制作的电化学发光传感器相比,离子液体为粘合剂的电化学发光传感器检测三丙胺的检出限降低1个数量级.海洛因对电化学发光传感器的发光信号有很好的增强作用,基于此建立了高灵敏度检测海洛因的电化学发光分析法,海洛因浓度与电化学发光信号在2.0×10~(-9)~2.0×10~(-5) mol/L范围内呈良好的线性关系,检出限为8×10~(-10) mol/L (S/N=3).将电化学发光传感器在5.0×10~(-9) mol/L海洛因溶液中采用

  12. Comparative study of ß-glucan induced respiratory burst measured by nitroblue tetrazolium assay and real-time luminol-enhanced chemiluminescence assay in common carp (Cyprinus carpio L.)

    DEFF Research Database (Denmark)

    Jiménez, Natalia Ivonne Vera; Pietretti, D.; Wiegertjes, G. F.;

    2013-01-01

    The respiratory burst is an important feature of the immune system. The increase in cellular oxygen uptake that marks the initiation of the respiratory burst is followed by the production of reactive oxygen species (ROS) such as superoxide anion and hydrogen peroxide which plays a role...... in the clearance of pathogens and tissue regeneration processes. Therefore, the respiratory burst and associated ROS constitute important indicators of fish health status. This paper compares two methods for quantitation of ROS produced during the respiratory burst in common carp: the widely used, single......-point measurement based on the intracellular reduction of nitroblue tetrazolium (NBT) and a real-time luminol-enhanced assay based on the detection of native chemiluminescence. Both assays allowed for detection of dose-dependent changes in magnitude of the respiratory burst response induced by β-glucans in head...

  13. Evaluation on performance of MAKER IS 1200 automated chemiluminescence analyzer%迈克IS1200全自动化学发光测定仪的性能评价

    Institute of Scientific and Technical Information of China (English)

    刘玉兰; 孙艳艳

    2016-01-01

    目的:对迈克IS1200全自动化学发光测定仪的性能进行评价。方法按照美国临床和实验室标准协会文件的要求,通过一系列实验设计,在IS1200上对乙肝5项、艾滋、丙肝和梅毒共8项进行检测,评价其精密度、试剂开瓶稳定性、线性和参考区间,与雅培i2000全自动化学发光分析仪的检测结果进行方法学比对。结果除乙型肝炎E抗原低值的总精密度略高于判断标准15%外,其余指标均符合要求;各项目的试剂开瓶稳定性良好;定量项目乙型肝炎表面抗体检测范围内的线性良好( r2>0.95);参考区间验证中,各项目均未发现离群值;方法学比对中,除丙型肝炎病毒抗体外,其他项目与雅培i2000全自动化学发光分析仪的相关性良好。结论迈克IS1200全自动化学发光测定仪的检测性能良好,满足实验室免疫检测工作的需求。%Objective To evaluate the performance of the MAKER IS1200 automated chemiluminescence analyzer .Methods A series of experiments were designed to evaluate the precision ,reagent open bottle stability ,linearity range and reference interval of the MAKER IS1200 automated chemiluminescence analyzer in 8 items of hepatitis B 5 indexes ,AIDS ,hepatitis C and syphilis ac‐cording to the requirements of CLSI documents ,the detection results were performed the methodological comparison with those de‐tected by Abbott i2000 automated chemiluminescence analyzer .Results Except the total precision of HBEAG low value was slight‐ly higher than 15% of the judgment standard ,other indexes conformed to the requirements ;the open bottle stability of various items of reagent was better ;the quantitative item HBSAB had good linearity within the detection range (r2 >0 .95);in the verification of reference interval ,no outlier was found in each item ;in the methodological comparison ,the other items had good correlation with those detected by the ABBOTT i

  14. 高锰酸钾-鲁米诺化学发光体系测定香草醛%Determination of vanillin with potassium permanganate-luminol chemiluminescence system

    Institute of Scientific and Technical Information of China (English)

    周艳梅; 王亚萍; 马同森; 谢晓龙

    2012-01-01

    Based on the fact that the chemiluminescence (CL) reaction of potassium permanganate and luminol in alkaline medium could be enhanced by vanillin, a new analytical method of chemiluminescence to detect vanillin was developed, which expands the application of flow-injection chemiluminescence in food analysis. The effect of type of medium and concentration of reagents on CL intensity was investigated. The results showed that the optimum concentrations of NaOH, KMnO4 and Lumino were 0.20 mol/L, 3. 0 × 10 -5 mol/L and 7. 0 × 10-5 mol/L, respectively. Under the optimum conditions, linearity relationship of values of CL intensity and mass concentration of vanillin was obtained in the range of 5. 0× 10-8 ~3. 0 × 10-6g/mL, with a equation of linear regression as A/ = 7. 188 3 × 108 C( g/mL) + 71. 767 4 (r = 0.994 2). Parallel determination of 5. 0 × 10-7 g/mL vanillin for 11 times show that the RSD is 1.3% with a detection limit of 1.1 × 10-8 g/mL. A new method of flow-injection chemiluminescence to detect vanillin was found, which has been successfully applied to the determination of vanillin in pill and the recovery rate obtained were in the range of 98% -104%.%为了拓展流动注射化学发光法在食品检测中的应用,利用草醛能够增敏NaOH介质中高锰酸钾—鲁米诺化学发光体系的发光信号的特点,建立了化学发光测定香草醛的方法.文中考察了介质浓度、发光试剂浓度等因素对发光体系信号的影响,确定了测定香草醛的最佳条件:介质NaOH浓度为0.20 mol/L,高锰酸钾浓度为3.0×10-5 mol/L,鲁米诺浓度为7.0×10-5mol/L.在优化实验条件下,体系的发光强度与香草醛浓度在5.0×10-8~3.0×10-6g/mL内呈线性关系,标准曲线:△I=7.1883 ×108C(g/mL) +71.767 4(r =0.994 2).对5.0×10-7 g/mL的香草醛平行测定11次,相对偏差为1.3%,其检出限为1.1 × 10-8g/mL.建立了测定香草醛的流动注射化学发光法新方法,并成功地测定了片剂中香

  15. Ascorbic acid-(fluorescein + CTMAB + Cu2+) chemiluminescence detection of urine uric acid%抗坏血酸-(荧光素+CTMAB+Cu2+)化学发光检测尿液的尿酸

    Institute of Scientific and Technical Information of China (English)

    鲁芳; 毛青青; 吕家根

    2014-01-01

    检测人尿液中尿酸含量的化学发光新方法,试剂稳定、无毒,不使用鲁米诺。在碱性条件下,尿酸选择性地猝灭抗坏血酸与荧光素-十六烷基三甲基溴化铵-铜离子混合物体系的化学发光,尿样直接稀释后化学发光检测尿酸含量。尿酸浓度在0.5~10 mg/L的范围内与化学发光信号具有良好的线性关系,线性回归方程为ΔI =306.6 c+10.904(n=5,R2=0.990),最低检出限为0.06 mg/L(3σ),定量检出限为0.2 mg/L(10σ),对浓度1 mg/L的尿酸平行测定n=11,RSD为1.2%。将本法用于人体尿液中尿酸含量的测定,与临床测定结果进行了对比,并进行了回收率实验,说明本方法具有较好的准确性和选择性;结合流动注射技术操作简便,对临床检验有参考价值和转化应用潜质。%Development of new luminol free chemiluminescence method for determination of uric acid in human urine with the use of non-toxic and stable chemiluminescent reagents. In alkaline solution,chemi-luminescence produced by ascorbic acid reacting with fluorescein-CTMAB-copper ion can be selectively quenched by the presence of uric acid,diluted urine samples were analyzed by proposed chemilumines-cence method. The uric acid concentration in the range of 0. 5 to 10 mg/L and chemiluminescence signal has a good liner relationship,obeyed the equation △I=306. 6 c+10. 904(n=5,R2 =0. 990)with the lowest detection limit of 0 . 06 mg/L( 3σ)and quantification limit of 0 . 2 mg/L( 10σ). The relative standard deviation(RSD)was 1. 2%(n=11)when determining 1. 0 mg/L uric acid standard. By meas-uring the real urine samples in contrast to the clinical results,as well as by the recovery test,the proposed method presented acceptable selectivity,accuracy and sensitivity for the determination of uric acid. In combination with flow injection,the new method achieved simple and robust analytical operation. As such, this method implies its potential

  16. Determination of antioxidation of apple polyphenols by flow injection chemiluminescence%流动注射化学发光测定苹果多酚抗氧化活性

    Institute of Scientific and Technical Information of China (English)

    刘彩红; 李玉琴; 李珂; 贾宝秀; 郭玉蓉

    2012-01-01

    用流动注射化学发光法测定苹果多酚的体外抗氧化作用。将苹果多酚提取液加入3种化学发光体系,测量其发光强度,根据系统化学发光被抑制的程度评价苹果多酚对活性氧自由基的清除能力,并以抗坏血酸(VC)为阳性对照。结果表明,苹果多酚对三种活性氧自由基(O2-·、·OH、H2O2)的清除能力远强于VC;对H2O2和·OH的清除能力相当,强于对O2-·的清除能力。结果显示苹果多酚具有很好的抗氧化能力。%The purpose was to determine the scavenging ability on ROS free radical of apple polyphenols in vitro by flow injection chemiluminescence.The determination was based on the inhibition effect of apple polyphenols extraction in three chemiluminescence system,and vitamin C was used as positive control.It was showed that apple polyphenols original liquid can inhibit and clear the ROS such as O2-·,·OH and H2O2 effectively,and the scavenging ability was better than vitamin C.The scavenging ability of apple polyphenols on H2O2 was much better than O2-·,but was equal to ·OH.Conclusion was the apple polyphenols extraction was an excellent natural antioxidant.

  17. The fit of four parameter logistic calibration of DXI 800 chemiluminescence analyzer by Origin software%采用Origin软件计算DXI 800发光仪的四参数logistic定标数据

    Institute of Scientific and Technical Information of China (English)

    王强; 马小红; 翁秀妹

    2014-01-01

    Objective To fit 4 parameter logistic calibration of DXI 800 chemiluminescence analyzer by Origin software and compare the datum difference between Origin and DXI 800,and to investigate the calculation possibility of unknown results of DXI 800 by Origin software.Methods The calibration results of testosterone,total thyroxine, estradiol,troponin I,thyrotropic hormone,carcino-embryonic antigen and progesterone were collected and fit by Origin software,and the calculation results with the original results of DXI 800 system were analyzed comparatively.Results The calculation results of Origin software were very close to the original results of DXI 800,and mean bias was from-0.85% to 1 .68%.Conclusions Origin software is suitable for the calculation of unknown results of DXI 800 chemiluminescence analyzer.%目的:采用Origin软件拟合DXI 800发光仪的四参数logistic定标数据,比较其与DXI 800计算的数据差异,探讨采用Origin软件计算DXI 800未知数据的可能性。方法选择睾酮、总甲状腺素、雌二醇、肌钙蛋白I、促甲状腺激素、癌胚抗原和孕酮的定标数据,采用Origin软件拟合四参数logistic计算结果,与DXI 800发光仪计算的结果比较。结果采用 Origin 软件计算的结果与 DXI 800计算的结果基本一致,平均偏倚介于-0.85%~1.68%之间。结论可以采用Origin软件计算DXI 800发光仪的未知结果。

  18. Development and optimization of an analytical method for the determination of Sudan dyes in hot chilli pepper by high-performance liquid chromatography with on-line electrogenerated BrO- -luminol chemiluminescence detection.

    Science.gov (United States)

    Zhang, Yantu; Zhang, Zhujun; Sun, Yonghua

    2006-09-29

    The determination of four Sudan dyes by means of high-performance liquid chromatography (HPLC) with chemiluminescence (CL) detection was proposed. The method was based on the enhancement effect of Sudan dyes on the chemiluminescence reaction between luminol and BrO-, which was on-line electrogenerated by constant current electrolysis. The separation was carried out on Nucleosil RP-C18 column (250 mm x 4.6 mm i.d., 5 microm, pore size, 100 A) at 35 degrees C. The mobile phase consisted of a V (methanol): V (0.2% aqueous formic acid) = 90:10 solution. At a flow-rate of 1.0 ml min(-1), the total run time was 25 min. The effects of several parameters on the HPLC resolution and CL emission were studied systematically. For the four Sudan dyes, the limits of detection (LOD) at a signal-to-noise of 3 ranged from 4 to 8 microg kg(-1) and the limits of quantification (LOQ) at a signal-to-noise of 10 ranged from 13 to 27 microg kg(-1). The relative standard deviations (RSD) of intra-and inter-day precision were below 4.4%. The average recoveries for all four Sudan dyes (spiked at the levels of 1.0 and 1.5 mg kg(-1)) in chilli tomato sauce and hot chilli pepper ranged from 94% to 105%, and the relative standard deviations of the quantitative results were from 2.5 to 4.2%. The proposed method had been successfully applied to the determination of four Sudan dyes in hot chilli products.

  19. 应用CLSI文件对化学发光免疫分析系统性能的初步评价%Preliminary Evaluation of the Performance of the Chemiluminescent Immunoassay System by CLSI File

    Institute of Scientific and Technical Information of China (English)

    张帮林; 郭永灿

    2014-01-01

    目的:评价BECKMAN ACCESS2全自动微粒子化学发光免疫分析系统相关性能。方法:以测定游离三碘甲状腺原氨酸(FT3)为例,收集患者高、中、低值新鲜血清混合标本,应用CLSI颁布的EP10-A2对ACCESS2全自动化学发光免疫分析系统进行初步评价。结果:通过CLSI EP10-A2程序评价显示ACCESS2检测系统偏差、不精密度、交叉污染、线性漂移等因素都在可接受范围内,对精密度无明显影响。结论:ACCESS2全自动发光免疫分析系统分析性能可靠,适合临床实验室使用。%Objective:To evaluate the performance of Beckman Access2 automated chemiluminescent immunoassay system in order to know its status. Method:Using the CLSI document EP10-2A for preliminary evaluation methods to approve the high,medium and low fresh serum of patients which were adopted as samples and was assayed FT3 in Beckman Access2 testing system.Result:The factors of carryover and drift,system deviation,imprecision,cross contamination in the ballpark,they had no effect on precision.Conclusion:The performance of Beckman Access2 automated chemiluminescent system is reliable and suitable for clinical laboratory to use.

  20. 化学发光法检测梅毒螺旋体抗体的临床价值研究%Study on the Clinical Value of Chemiluminescence Detection of Syphilis Antibody

    Institute of Scientific and Technical Information of China (English)

    王伟诗

    2016-01-01

    目的:研究分析化学发光法检测梅毒螺旋体抗体的临床价值。方法将2823份标本分别采用梅毒螺旋体明胶凝集试验(TPPA)、酶联免疫吸附实验(ELISA)和化学发光法(CLIA)检测梅毒螺旋体特异性抗体。结果2823份血清标本中,TPPA方法检测出梅毒螺旋体特异性抗体阳性94例,阳性检出率为3.33%,以该方法检测结果为标准;CLIA法同TPPA方法检测结果相比,阳性符合率为98.94%,阴性符合率为99.63%,总符合率为99.61%;ELISA法同TPPA方法比较,阳性符合率为91.49%,阴性符合率为99.45%,总符合率为99.19%。结论化学发光法检测梅毒螺旋体抗体具有较高的灵敏性和特异性,结果客观,易分析等优点,能够为梅毒血清学临床诊断提供科学依据。%Objective To explore the clinical application value of chemiluminescent immunoassay (CLIA) in screening treponema pallidum antibody.Methods 2 823 specimens were tested by syphilis spiral gelatin agglutination test (TPPA), enzyme linked immunosorbent assay (ELISA) and chemiluminescence method (CLIA).Results 2 823 serum specimens, detection of treponema pallidum specific antibody was positive in 94 cases of TPPA method, the positive rate was 3.33%, the detection results of the method as the standard, results compared with the TPPA method of CLIA assay, the positive coincidence rate was 98.94%, the negative coincidence rate was 99.63%, the total coincidence rate was 99.61%, ELISA was the same as the TPPA method, the positive coincidence rate was 91.49%, the negative coincidence rate was 99.45%, the total coincidence rate was 99.19%.Conclusion The detection of syphilis antibody with chemiluminescence immunoassay detection has high sensitivity and specificity, and it can provide scientific basis for clinical diagnosis of syphilis.

  1. 恒电位电解流动注射化学发光分析法测定钢铁中微量钼%Determination of micro molybdenum in steel by constant potential electrolysis-flow injection chemiluminescence analysis

    Institute of Scientific and Technical Information of China (English)

    杨玲娟; 谢天柱; 雷新有

    2011-01-01

    建立了钼的恒电位电解流动注射化学发光分析法.在0.02 mol/L的H2 C2 O4酸度下,使含不具发光活性钼(Ⅵ)的溶液,以1.7 mL/min的流速通过自制的流通式碳电解池时,在-0.60 V(vs∶Ag/AgCl)电位处,钼(Ⅵ)在线还原为钼(Ⅲ),钼(Ⅲ)与鲁米诺在碱性条件下产生化学发光,且发光强度与钼的质量浓度在5.0× 10-10~5.0×10-7 g/mL范围内呈线性关系,钼(Ⅵ)检出限为5×10-11 g/mL.大多数常见的阳离子和阴离子对钼的测定没有干扰,Fe3+和Fe2+的允许量较低,但试液通过测定流路中钠型离子交换柱后,Fe3+和Fe2+的允许量提高到1 000倍.方法已用于低合金钢和碳钢标准样品中微量钼的测定,测定值与认定值一致,相对标准偏差在0.68%~1.3%之间.%The determination method of molybdenum by constant potential electrolysis - flow injection chemiluminescence analysis was established. In 0.02 mol/L H2C2O4 solution, the molybdenum(Ⅵ) solution without luminescence activity passed through self-made flow-type carbon electrolysis cell at the rate of 1. 7 mL/min. Molybdenum(VD was on-line reduced to molybdenum(Ⅲ) at the potential of -0. 60 V(vs. Ag/AgCl). Molybdenum (Ⅲ) generated chemiluminescence with luminol under alkaline conditions. The chemiluminescence intensity was linear to mass concentration of molybdenum in the range of 5. 0× 10-10 - 5. 0× 10~7 g/mL. The detection limit of molybdenum(Ⅵ) was 5 × 10-11 g/mL. Most common positive and negative ions had no interference with the determination of molybdenum. The permissible amount of Fe3+ and Fe2+ was small. However, it could be increased to 1 000 times after the test solution passed through Na-type ion exchange column in the determination flow path. The proposed method was applied to the determination of micro molybdenum in certified reference materials of low alloy steel and carbon steel. The determination results were consistent with the certified values, with relative standard

  2. Detection of Matrix Metalloproteinase-11in Menstrual Blood by Enhanced Chemiluminescence Method%增强化学发光法检测基质金属蛋白酶-11鉴定月经血

    Institute of Scientific and Technical Information of China (English)

    章雅清; 陆惠玲; 姚亚楠

    2012-01-01

    目的 探讨增强化学发光法检测人基质金属蛋白酶-11(matrix metalloproteinase-11,MMP- 11)鉴定月经血的法医学应用价值.方法 运用增强化学发光法检测月经血痕、阴道拭子、外周血痕、唾液斑、尿液斑和精液斑中的MMP-11,考察MMP-11的组织特异性、稳定性及检测方法的灵敏度.结果 月经血痕中MMP-11蛋白的检测阳性率为89.47%,阴道拭子、外周血痕、唾液斑、尿液斑和精液斑中均未检出MMP-11.当上样量为25 μL时,蛋白质量浓度为1.329 μg/μL,可检出MMP-11.4℃放置20个月的MMP-11阳性的月经血痕,检测阳性率为89.58%.结论 用增强化学发光法检测MMP- 11蛋白的方法灵敏度高、特异性好,能用于月经血与外周血、阴道液等常见斑痕的鉴别.%Objective To explore the forensic application value of detection of matrix metalloproteinase-11 (MMP-11) in menstrual blood by enhanced chemiluminescence method. Methods Menstrual blood, vaginal swab, peripheral blood, saliva stain, urine stain and semen stain were collected to detect whether or not there were MMP-11 using enhanced chemiluminescence method. The specificity and reliability of the MMP-11 assay along with its sensitivity were evaluated. Results The positive detection rate of MMP-11 in menstrual blood was 89.47%, whereas no MMP-11 was found in vaginal swab, peripheral blood, saliva stain, urine stain and semen stain. When 25 μL sample was added, the mass concentration of protein was 1.329 μg/μL, then MMP-11 could be detected. A positive detection rate of 89.58% was observed in MMP-11 positive menstrual blood samples after stored at 4℃ for 20 months. Conclusion Enhanced chemiluminescence method is sensitive and specific for detecting MMP-11, and can be applied to distinguish menstrual blood from common stain such as peripheral blood, vaginal fluid.

  3. 高灵敏度化学发光免疫法检测人血清中促甲状腺激素%A Highly Sensitive Chemiluminescence Immunoassay Technique for Assaying TSH in Human Serum

    Institute of Scientific and Technical Information of China (English)

    齐素文; 张会生; 刘昕宇; 李乔亮

    2013-01-01

    A highly sensitive magnetic particle-based chemiluminescence immunoassay (CLIA) technique for assaying thyroid stimulating hormone (TSH) in serum was described.Fluorescein isothiocyanate (FITC) and N-(aminobutyl)-N-(ethylisoluminol) (ABEI) were used to label two different monoclonal antibodies of anti-TSH.Both of the labeled antibodies combined with TSH to form a sandwiched immunoreaction that was monitored by chemiluminescence (CL) detection.The magnetic particles (MPs) that were coated with anti-FITC antibody served as both the solid phase and the separator.The method showed high sensitivty with a detection limit of 0.01 μIU/mL.The coefficient of variation of intra-assay imprecision and the inter-assay imprecision were less than 10%.The average recoveries were 96% ~ 103%.A correlation analysis against an established automated assay generated a correlation coefficient of 0.993 7.The proposed method demonstrates an acceptable performance for quantifying serum TSH and is suitable for the clinical lab.%建立了一种高灵敏度检测促甲状腺激素(TSH)的化学发光体系.将异鲁米诺(ABEI)和异硫氰酸荧光素(FITC)分别标记TSH的单克隆抗体,与待测TSH通过夹心法进行免疫反应形成抗原抗体复合物之后,加入抗-FITC抗体包被的磁珠,在磁场作用下加入缓冲液进行清洗,分离非特异性物质,加入发光底物NaOH和H2O2,检测发光强度(RLU).本方法的灵敏度为0.01 μIU/mL,批内和批间变异系数均在10%之内,回收率在96% ~ 103%之间.使用本方法与其他检测系统进行比对,相关系数为0.993 7.实验结果表明,基于ABEI发光检测TSH的化学方法灵敏度高、结果可靠,具有较高的应用价值.

  4. Determination of squamous cell carcinoma antigen based on the magnetic particles chemiluminescence immunoassay%基于磁颗粒化学发光免疫分析方法检测鳞状细胞癌抗原∗

    Institute of Scientific and Technical Information of China (English)

    田峰; 齐素文

    2015-01-01

    目的:建立一种快速、灵敏的化学发光免疫分析方法检测人血清中鳞状细胞癌抗原(SCCA)水平。方法使用异鲁米诺(ABEI)和异硫氰酸荧光素(FITC)分别标记 SCCA 的单克隆抗体,与待测 SCCA 抗原通过夹心法免疫反应形成抗原抗体复合物,采用包被有 FITC 的磁颗粒作为固相分离载体,加入底物之后检测发光强度。结果本方法线性范围达到22 ng/mL,灵敏度为0.025 ng/mL,批内变异系数(CV)和批间 CV 分别小于6%和7%。与现有的 SCCA 检测方法进行比对,相关系数为0.9901。结论基于磁颗粒化学发光免疫分析检测 SCCA 的方法性能稳定、可靠,可用于定量检测人血清中 SCCA 浓度。%Objective To establish a rapid and sensitive chemiluminescence immunoassay for detecting squamous cell carcinoma antigen (SCCA)in human serum.Methods Fluorescein isothiocyanate (FITC)and N-(aminobutyl)-N-(ethylisoluminol)(ABEI) were used to label two different monoclonal antibodies of anti-SCCA.SCCA in serum combined with labeled antibodies and formed a sandwiched immunoreaction.After adding the substrate solution,the relative light unit of ABEI was measured.Magnetic particles coated with anti-FITC antibody were used as solid separation carrier.Results The results demonstrated that the method was linear to 22 ng/mL with a detection limit of 0.025 ng/mL.The coefficient of variation (CV)was less than 6% and 7% for intra-assay and inter-assay precision,respectively.Compared with the commercial kit,the proposed method showed a correlation of 0.990 1.Conclu-sion chemiluminescence immunoassay based on magnetic particles displays acceptable performance for quantification of SCCA and is appropriate for use in clinical diagnosis.

  5. 基于微孔板化学发光法检测碱性磷酸酶的研究%Research of Chemiluminescent Assay of Alkaline Phosphatase Based on Microplate

    Institute of Scientific and Technical Information of China (English)

    王宇; 王宗良; 崔立国; 庄秀丽; 陈学思; 章培标

    2012-01-01

    CDP-Star能被ALP迅速水解,且水解产物能够持续发光.本研究对化学发光法检测ALP的反应条件进行了优化.优化后的反应条件为:125 μtmol/L的CDP- Star在pH 9.5缓冲液中,37℃下与ALP反应10min.在此条件下,△RLU与ALP的活力呈线性关系.因此,建立了一种基于微孔板化学发光法检测ALP的实验方法.结果表明,本方法检测ALP的检测线性范围是0.05~10 U/L和10~1000 U/L,相对于光吸收法具有速度快、灵敏度高、重复性好等优点.因此,本方法可以被开发成试剂盒,应用于高通量全自动生化分析仪的测试分析.%Enzymatic dephosphorylation of CDP-Star by ALP leads to CDP-Star decompose and continuously emit light. Optimal conditions for the determination of ALP by chemiluminescent method were investigated in this research. 125 μmol/L CDP-Star reacted with ALP in the buffer solution of pH = 9. 5 and incubated for 10 min at 37℃ were selected as the optimal conditions for the determination of ALP. The amount of ARLU is proportional to ALP activity. Therefore, the method of chemiluminescent assay of ALP based on microplate was established. The results showed that the linear range for the determination of ALP was 0. 05 -10 U/L and 10-1000 U/L. This method was more rapid, sensitive and reproducible than that of traditional colorimetric method. Therefore, this method can be developed into a kit used in the high-throughput automatic biochemical analyzer.

  6. An enhanced cerium(IV)-rhodamine 6G chemiluminescence system using guest-host interactions in a lab-on-a-chip platform for estimating the total phenolic content in food samples.

    Science.gov (United States)

    Al Haddabi, Buthaina; Al Lawati, Haider A J; Suliman, FakhrEldin O

    2016-04-01

    Two chemiluminescence-microfluidic (CL-MF) systems, e.g., Ce(IV)-rhodamine B (RB) and Ce(IV)-rhodamine 6G (R6G), for the determination of the total phenolic content in teas and some sweeteners were evaluated. The results indicated that the Ce(IV)-R6G system was more sensitive in comparison to the Ce(IV)-RB CL system. Therefore, a simple (CL-MF) method based on the CL of Ce(IV)-R6G was developed, and the sensitivity, selectivity and stability of this system were evaluated. Selected phenolic compounds (PCs), such as quercetin (QRC), catechin (CAT), rutin (RUT), gallic acid (GA), caffeic acid (CA) and syringic acid (SA), produced analytically useful chemiluminescence signals with low detection limits ranging from 0.35 nmol L(-1) for QRC to 11.31 nmol L(-1) for SA. The mixing sequence and the chip design were crucial, as the sensitivity and reproducibility could be substantially affected by these two factors. In addition, the anionic surfactant (i.e., sodium dodecyl sulfate (SDS)) can significantly enhance the CL signal intensity by as much as 300% for the QRC solution. Spectroscopic studies indicated that the enhancement was due to a strong guest-host interaction between the cationic R6G molecules and the anionic amphiphilic environment. Other parameters that could affect the CL intensities of the PCs were carefully optimized. Finally, the method was successfully applied to tea and sweetener samples. Six different tea samples exhibited total phenolic/antioxidant levels from 7.32 to 13.5 g per 100g of sample with respect to GA. Four different sweetener samples were also analyzed and exhibited total phenolic/antioxidant levels from 500.9 to 3422.9 mg kg(-1) with respect to GA. The method was selective, rapid and sensitive when used to estimate the total phenolic/antioxidant level, and the results were in good agreement with those reported for honey and tea samples.

  7. Evaluation of luminol chemiluminescence based on simultaneous introducing of coumarin derivatives as green fluorophores and chitosan-induced Au/Ag alloy nanoparticle as catalyst for the sensitive determination of glucose.

    Science.gov (United States)

    Chaichi, M J; Alijanpour, S O; Asghari, S; Shadlou, S

    2015-03-01

    We report herein the development of a novel chemiluminescence system based on simultaneous introducing of synthetic coumarin derivatives and chitosan-induced Au/Ag alloy NPs on the luminol CL system and suggest how it may be useful for determination of glucose. Chitosan-induced Au/Ag nanoalloys in the coumarin derivatives intensified-luminol CL system, in addition to catalyze CL reaction can make a change in the process of coumarin derivatives effect as fluorophore on the luminol CL system. This phenomenon is caused by interaction between active functional groups of coumarin derivatives and chitosan. The interaction strength depends on the coumarin derivatives' structure and their substituents. Considering the inevitable trend luminol radical and superoxide anion radical to absorption on the surface of the embedded Au/Ag nanoalloy in the chitosan matrix, it can be concluded that chitosan acts as a platform for all reagents involved in the CL reaction including coumarin derivatives, Au/Ag nanoalloy and luminol, and electron-transfer taking place on it; Placing all chemiluminescent reagents together on the chitosan network can lead to a powerful CL due to increasing rigidity of CL system. The most efficient coumarin derivative on the Au/Ag nanoalloy-fluorophore-luminol-H2O2 CL system, in relation to interaction capability with chitosan' functional groups, was selected and the CL condition in presence of it was optimized. Whereas the glucose oxidase-mediated oxidation of glucose yields gluconic acid and H2O2, under optimum condition the most efficient CL system was applied to detection of glucose due to enzymatically production of hydrogen peroxide. The linear response range of 1.5 × 10(-6)-5.0 × 10(-3) M and the detection limit (defined as the concentration that could be detected at the signal-to-noise ratio of 3) of 7.5 × 10(-7) M was found for the glucose standards. Also, the developed method was successfully applied to determination of glucose in

  8. Hypericin from St. John's Wort (hypericum perforatum) as a novel natural fluorophore for chemiluminescence reaction of bis (2,4,6-trichlorophenyl) oxalate-H{sub 2}O{sub 2}-imidazole and quenching effect of some natural lipophilic hydrogen peroxide scavengers

    Energy Technology Data Exchange (ETDEWEB)

    Kazemi, Sayed Yahya [Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari (Iran, Islamic Republic of); Abedirad, Seyed Mohammad, E-mail: mabedirad@yahoo.com [Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari (Iran, Islamic Republic of); Zali, Seyed Hassan; Amiri, Mohadeseh [Department of Range and Watershed Management, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari (Iran, Islamic Republic of)

    2012-05-15

    Hypericin (HYP) molecule is a natural photoactive pigment, which plays a role as an effective photoreceptor in some plants of the Hypericum species (the most common of which is Saint John's Wort) and some insect species. The present work deals with the first attempt to the study of peroxyoxalate chemiluminescence (POCL) system in the presense of HYP as a natural fluorophore. Reaction of bis (2,4,6-trichlorophenyl) oxalate(TCPO)-H{sub 2}O{sub 2}-imidazole can transfer energy to a HYP via formation of dioxetane through the chemically initiated electron exchange luminescence (CIEEL) mechanism and can emits a very intense red light. The effects of HYP, hydrogen peroxide, TCPO and imidazole concentrations on kinetic chemiluminescence parameters were also studied. These parameters including rise and fall rate constant for the chemiluminescence burst, theoretical and experimental maximum intensity, theoretical and experimental time to reach maximum intensity and total light yield emission were evaluated by using a pooled intermediate model for a non-linear least-squares curve fitting program, KINFIT. Moreover, quenching effect of two lipophilic natural antioxidant, Quercetin and {beta}-carotene on it system was also investigated. The measurable concentration range of 7 Multiplication-Sign 10{sup -6} M to 7.5 Multiplication-Sign 10{sup -5} M of antioxidants were evaluated from the proper Stern-Volmer plots with satisfactory RSD% and corresponding detection limits of 2.2 Multiplication-Sign 10{sup -6} and 3.7 Multiplication-Sign 10{sup -6} for {beta}-carotene and quercetin respectively. - Highlights: Black-Right-Pointing-Pointer Red fluorophores may therefore chemiluminescence more intensely than other commonly chemiluminophores and emits light in longer wavelengths. Black-Right-Pointing-Pointer Hypericin from St. John's wort (hypericum perforatum) as natural red fluorophore for peroxyoxalate chemiluminescence was introduced. Black-Right-Pointing-Pointer Quenching

  9. Speciation of As(III)/As(V) in water samples by a magnetic solid phase extraction based on Fe₃O₄/Mg-Al layered double hydroxide nano-hybrid followed by chemiluminescence detection.

    Science.gov (United States)

    Abdolmohammad-Zadeh, Hossein; Talleb, Zeynab

    2014-10-01

    A novel magnetic solid phase extraction method was developed for the speciation of As(III)/As(V) in aqueous solutions utilizing Fe3O4-doped Mg-Al layered double hydroxide (LDH) as a nano-sorbent. The method is based on the separation and pre-concentration of As(V) by Fe3O4/Mg-Al LDH nano-hybrid prior to determination by a chemiluminescence (CL) technique. The CL route involves the oxidation of luminol by vanadomolybdoarsenate heteropoly acid in a basic media. Since the existing cations cannot be adsorbed by positively charged layers of the LDH and other potentially interferent anions had no considerable effect on the CL reaction, it provides a very selective and sensitive determination approach for As(V). The determination of total arsenic and hence indirectly As(III) involve the pre-oxidation of As(III) to As(V) by a mixture of hydrogen peroxide and potassium hydroxide. Several factors affecting the extraction and determination of the analyte were investigated and optimized. Under optimum conditions, the calibration graph was linear in the range of 5.0-5000 ng L(-1). The limit of detection and enrichment factor was 2.0 ng L(-1) and 80, respectively. The method was validated by the analysis of a standard reference material (NIST SRM 1643e), and successfully applied to the speciation of arsenic in several water samples with recoveries in the range of 93.3-106.7% for the spiked samples.

  10. 催化裂化油浆中硫化物气相色谱分析%Determination of Sulfur Compounds in Catalytic Cracking Slurry by Gas Chromatography With Sulfur Chemiluminescence Detection

    Institute of Scientific and Technical Information of China (English)

    何俊辉; 贾广信; 黎爱群; 薛晓军

    2014-01-01

    采用气相色谱配硫化学发光检测器(SCD)结合高温模拟蒸馏ASTM D7169-05方法对巴陵石化催化裂化油浆中含硫化合物进行了分析鉴定,结果表明:油浆的馏程在253℃到690℃左右范围内,硫化物类型主要是二苯并噻吩类和萘并噻吩类化合物;定量分析结果表明,萘并噻吩类化合物占油浆中总硫化物含量的70%以上。%The catalytic cracking slurry was analyzed by gas chromatography with sulfur chemiluminescence detection (GC-SCD) and SimDis ASTM D7169-05 method. The results show that distillation range of the slurry is from 253 ℃to 690 ℃,and main sulfide types are dibenzothiophenes and naphthothiophenes. Quantitative analysis results show that naphthothiophene compounds account for more than 70%of the total sulfur content in the slurry.

  11. Effect of nonionic fluorosurfactant on the electrogenerated chemiluminescence of the tris(2,2'-bipyridine)ruthenium(II)/tri-n-propylamine system: lower oxidation potential and higher emission intensity.

    Science.gov (United States)

    Li, Feng; Zu, Yanbing

    2004-03-15

    Fluorosurfactants are commercially available, and their applications in electrochemical systems have been the interest of many studies. Here, we describe a novel effect of a nonionic fluorosurfactant (Zonyl FSN) on the electrogenerated chemiluminescence (ECL) of the tris(2,2'-bipyridine)ruthenium(II)/tri-n-propylamine (TPrA) system at gold and platinum electrodes. Compared with its hydrocarbon analogue (Triton X-100), the adsorbed fluorosurfactant species not only rendered the electrode surfaces more hydrophobic but also significantly retarded the growth of the electrode oxide layers. As a result, more facile direct oxidation of TPrA was achieved, which led to the appearance of a low oxidation potential ECL signal (below 1.0 V vs SCE). At the gold electrode, the ECL peak appeared at 0.82 V, approximately 400 mV more negative than usual; while its intensity was approximately 50 times higher. The generation of the intense ECL signal at low oxidation potential may lead to the development of more efficient ECL analysis.

  12. Detection of total biogenic amines in foods by chemiluminescence biosensor%化学发光生物传感器检测食品中生物胺总量

    Institute of Scientific and Technical Information of China (English)

    唐晗; 王晓朋; 吴中波; 万德慧; 刘晓宇

    2014-01-01

    目的:建立快速地有效检测食品中生物胺总量的分析方法。方法用固定化的二胺氧化酶制成酶柱作为生物传感器的识别元件,将微流控芯片与化学发光仪结合作为检测元件,以鲁米诺-铁氰化钾作为化学发光体系,通过流动分析法来检测食品中生物胺的总量。结果当腐胺、组胺、酪胺浓度分别在3~500、2~100、3~200μmol/L时,线性回归方程分别为Y=17.448X+408.93、Y=88.329X+997.13、Y=45.762X+1728.2,相关系数分别为0.9966、0.9937、0.9907,检出限分别为0.7、0.5、0.5μmol/L。另外,本研究对传感器的性能进行了评价。结果显示,在最佳条件下对含有腐胺、组胺、酪胺的溶液分别测定7次,其RSD均小于8%,精密度较好;以腐胺为底物,连续通入鲁米诺和铁氰化钾溶液7次, RSD=1.66%,表现出良好的稳定性;将固定化酶保存在磷酸盐缓冲液中,每10 d测定一次酶活,持续70 d。2个月内固定化二胺氧化酶酶活仅降低了15%,保存时间较长;将传感器用于检测猪肉、鲫鱼和葡萄酒中的生物胺总量,样品中三种胺的添加回收率均在90%~94%之间,回收率较高,适合检测总生物胺。结论此传感器的综合性能良好,适用于食品中生物胺总量的快速检测。%Objective To establish a method for the analysis of the biogenic amines content in foods. Methods A novel micro-fluidic chip-chemiluminescence-enzyme biosensor was designed and assembled to determine the total biogenic amines content in foods. Commercial diamine oxidase was used as biological recognition element of the sensor, the micro-fluidic chip with chemiluminescence analyzer was used as a sensing element, and luminol-ferricyanide was used in chemiluminescence system. Results When the concentration of putrescine, histamine, and tyramine were 3~500, 2~100, 3~200μmol/L, the linear regression equation were Y=17.448X+408.93, Y=88.329X+997.13, Y=45.762X+1728.2, the correlation coefficient

  13. Determination of propoxur in environmental samples by automated solid-phase extraction followed by flow-injection analysis with tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection

    Energy Technology Data Exchange (ETDEWEB)

    Perez-Ruiz, Tomas [Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia (Spain)]. E-mail: tpr@um.es; Martinez-Lozano, Carmen [Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia (Spain); Garcia, Maria Dolores [Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia (Spain)

    2007-02-19

    A sensitive method for the analysis of propoxur in environmental samples has been developed. It involves an automated solid-phase extraction (SPE) procedure using a Gilson Aspec XLi and flow-injection analysis (FI) with chemiluminescence (CL) detection. The FI-CL system relies on the photolysis of propoxur by irradiation using a low-pressure mercury lamp (main spectral line 254 nm). The resultant methylamine is subsequently detected by CL using tris(2,2'-bipyridyl)ruthenium(III), which is on-line generated by photo-oxidation of the ruthenium(II) complex in the presence of peroxydisulfate. The linear concentration range of application was 0.05-5 {mu}g mL{sup -1} of propoxur, with a detection limit of 5 ng mL{sup -1}. The repeatability was 0.82% expressed as relative standard deviation (n = 10) and the reproducibility, studied on 5 consecutive days, was 2.1%. The sample throughput was 160 injection per hour. Propoxur residues below ng mL{sup -1} levels could be determined in environmental water samples when an SPE preconcentration device was coupled on-line with the FI system. This SPE-FI-CL arrangement provides a detection limit as low as 5 ng L{sup -1} using only 500 mL of sample. In the analysis of fruits and vegetables, the detection limit was about 10 {mu}g kg{sup -1}.

  14. Determination of propoxur in environmental samples by automated solid-phase extraction followed by flow-injection analysis with tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection.

    Science.gov (United States)

    Pérez-Ruiz, Tomás; Martínez-Lozano, Carmen; García, María Dolores

    2007-02-19

    A sensitive method for the analysis of propoxur in environmental samples has been developed. It involves an automated solid-phase extraction (SPE) procedure using a Gilson Aspec XLi and flow-injection analysis (FI) with chemiluminescence (CL) detection. The FI-CL system relies on the photolysis of propoxur by irradiation using a low-pressure mercury lamp (main spectral line 254 nm). The resultant methylamine is subsequently detected by CL using tris(2,2'-bipyridyl)ruthenium(III), which is on-line generated by photo-oxidation of the ruthenium(II) complex in the presence of peroxydisulfate. The linear concentration range of application was 0.05-5 microg mL(-1) of propoxur, with a detection limit of 5 ng mL(-1). The repeatability was 0.82% expressed as relative standard deviation (n=10) and the reproducibility, studied on 5 consecutive days, was 2.1%. The sample throughput was 160 injection per hour. Propoxur residues below ng mL(-1) levels could be determined in environmental water samples when an SPE preconcentration device was coupled on-line with the FI system. This SPE-FI-CL arrangement provides a detection limit as low as 5 ng L(-1) using only 500 mL of sample. In the analysis of fruits and vegetables, the detection limit was about 10 microg kg(-1).

  15. Detection of human serum free thyroxine by non-equilibrium competitive chemiluminesce immunoassay using FITC system%应用FITC系统建立非均衡竞争游离甲状腺素化学发光法

    Institute of Scientific and Technical Information of China (English)

    刘萍; 孔海霞; 张影; 田云霞; 宋启超; 涂值光

    2012-01-01

    [目的]制备抗人甲状腺素多克隆抗体并应用异硫氰酸荧光素(fluorescein isothiocyanate,FITC)系统建立非均衡竞争FT4化学发光法.[方法]以T4-牛血清白蛋白(bovin serum albumin,BSA)为抗原免疫新西兰兔,制备抗人T4多克隆抗体,亲和层析法吸附掉与3-碘-L-甲状腺原氨酸(3-iodo-L-tyrosine)、3,5-二碘-L-甲状腺原氨酸(3,5-diiodo-L-tyrosine)、D-三碘甲状腺原氨酸(D-T3)、L-三碘甲状腺原氨酸(L-T3)、反三碘甲状腺原氨酸(rT3)有交叉反应多抗,纯化特异性的抗人T4多克隆抗体并用FITC标记T4类似物,用抗FITC抗体包被微孔板.反应时,将FT4校准品或样品、FITC-T4类似物、T4抗体-HRP依次加入包被有抗FTTC抗体的化学发光板中,FITC -T4类似物与FT4校准品或样品,非均衡竞争结合T4抗体-HRP,从而在微孔板上形成免疫反应复合物T4类似物-T4抗体-HRP,通过酶促化学发光反应,就可以显示出与样品FT4浓度负相关的相对光单位(relative light unit,RLU),建立FT4化学发光检测方法.[结果]抗体经酶联免疫吸附实验证明对T4具有高度特异性,与3-iodo-L-tyrosine、3,5-diiodo-L-tyrosine、L-T3、D-T3、rT3无交叉反应.该方法建立的试剂盒标准曲线的线性相关系数不小于0.9900;分析灵敏度为0.9 pmol/L;线性检测范围0.9~130 pmol/L;精密度测试批内变异系数CV< 10%;与进口发光免疫分析系统做线性相关比较,线性回归系数为1.0852,相关系数为0.9673,临床符合率良好;本方法建立的试剂盒经加速稳定性试验37℃放置7d与2~8℃放置7d无异.[结论]综上所述,应用FITC系统建立的非均衡竞争FT4化学发光免疫分析灵敏度高、特异性好适于临床诊断应用.%[Objective]To prepare polyclonal antibodies(mAb) against human thyroxine and to establish a new method of non-equilibrium competitive chemiluminescence immunoassay (CLIA) with fluorescein isothiocyanate (FITC) for serum free thyroxine

  16. Chemiluminescent determination of H2O2 using 4-(1,2,4-triazol-1-yl)phenol as an enhancer based on the immobilization of horseradish peroxidase onto magnetic beads.

    Science.gov (United States)

    Yang, Xiaoyan; Guo, Yingshu; Mei, Zhenhua

    2009-10-01

    This article describes the employment of a novel p-phenol derivative, 4-(1,2,4-triazol-1-yl)phenol (TRP), as a highly potent signal enhancer of the luminol-hydrogen peroxide (H(2)O(2))-horseradish peroxidase (HRP) chemiluminescence (CL) system. The CL reaction conditions were optimized, and the enhancement characteristics of TRP were compared with those of p-iodophenol (PIP). TRP produced a strong enhancement of the CL with the effect of prolonging the light emission. The developed system was then applied to the determination of H(2)O(2) with immobilized HRP using magnetic beads as a solid support. The linear range for H(2)O(2) was 2.0x10(-6) to 1.0x10(-3) M. The detection limit for H(2)O(2) was 2.0x10(-6) M. The proposed sensor was applied successfully to the determination of H(2)O(2) in rainwater.

  17. Sensitized chemiluminescence of 2-phenyl-4,5-di(2-furyl)-1H-imidazole/K₃Fe(CN)₆/propyl gallate system combining with solid-phase extraction for the determination of propyl gallate in edible oil.

    Science.gov (United States)

    Kang, Jing; Han, Lu; Chen, Zhonglin; Shen, Jimin; Nan, Jun; Zhang, Yihua

    2014-09-15

    In this paper, a novel chemiluminescence (CL) method has been developed for the determination of propyl gallate (PG). The proposed method was based on the enhancing effect of PG on the CL signal of 2-phenyl-4,5-di(2-furyl)-1H-imidazole (PDFI) and K3Fe(CN)6 reaction in an alkaline solution. Under the optimum conditions, the enhanced CL intensity was linearly related to the concentration of PG. The linear range of the calibration curve was 0.05-8 μg/mL, and the corresponding detection limit (3σ) was 0.036 μg/mL. The relative standard deviation for determining 1.0 μg/mL PG was 2.8% (n=11). The proposed method has been successfully applied to the determination of PG in edible oil. The edible oil samples were prepared by the solid-phase extraction (SPE) with a C18 column served as the stationary phase. Furthermore, the possible CL mechanism was also discussed briefly based on the photoluminescence (PL) and CL spectra.

  18. Methodological evaluation of chemiluminescence microparticle immunoassay for determining HE4%化学发光微粒子免疫法检测人附睾蛋白方法学性能评价

    Institute of Scientific and Technical Information of China (English)

    郑桂喜; 王传新; 刘延红; 梁永媛

    2012-01-01

    目的 对Abbott Architect i2000SR检测系统应用化学发光微粒子免疫技术定量测定人附睾蛋白(HE4)的分析性能进行评价.方法 参考美国临床实验室标准化协会( NCCLS)文件,制定定量检测方法的方法学评价方案,通过Abbott Architect i2000sR检测系统测定HE4的批内精密度、批间精密度、线性范围、携带污染率和参考区间.结果 低、高值混合血清批内精密度CV分别为1.80%、2.34%;批间精密度CV分别为2.03%、2.70%;线性范围为19.8 ~1 573.5 pmol/L;携带污染率为-0.02%;参考区间为0.0~ 140.0 pmol/L.结论 Abbott Architect i2000SR检测系统测定HE4的精密度好、线性范围宽、携带污染率低,参考区间与厂家提供的一致,检测性能可满足临床要求.%To evaluate the performance methodology of Chemiluminescence microparticle immunoassay (CMIA) for determining HE4 using Abbott Architect i2000SR Chemiluminescence analyzer. Methods According to a method by NCCLS recommended, we designed the evaluation program of quantitative detection methods. The within-run precision、 between-run precision Jinear range,carry-over and biological reference interval of HE4 were determined. Results The coefficient of variation( CV) of within-run precision of low and high value pooled serum were respectively 1. 80% and 2. 34% . The CV of between-run precision were respectively 2. 03% and 2. 70% . The linear range was from 19.8 pmol/L to 1 573.5 pmol/L. The 1 carry-over was -0.02%. The biological reference interval was consistent with manufactures which is from 0. 0 pmol/L to 140. 0 pmol/L. Conclusion The within-run precision, between-run precision, linear range and carry-over of Abbott Architect i2000SR for determining HE4 meet the requirements of clinical use. The biological reference interval was consistent with that provided by the manufacturer.

  19. Improved accuracy of an LC-MS/MS method measuring 24R,25-dihydroxyvitamin D3 and 25-hydroxyvitamin D metabolites in serum using unspiked controls and its application to determining cross-reactivity of a chemiluminescent microparticle immunoassay.

    Science.gov (United States)

    Dowling, Kirsten G; Hull, George; Sundvall, Jouko; Lamberg-Allardt, Christel; Cashman, Kevin D

    2017-03-23

    Measurement of serum 25-hydroxyvitamin D [25(OH)D] is considered the best indicator of vitamin D status. Two minor vitamin D metabolites are common interferences encountered in 25(OH)D assays. The first is 3-epi-25-hydroxyvitamin D3 [3-epi-25(OH)D3], which if not chromatographically resolved from 25-hydroxyvitamin D3 [25(OH)D3], can overestimate 25(OH)D concentrations. The second is 24R,25-dihydroxyvitamin D3 [24R,25(OH)2D3], which can cross-react with the antibodies in 25(OH)D immunoassays. Our aim was to develop an LC-MS/MS method capable of detecting both 3-epi-25(OH)D3 and 24R,25(OH)2D3 in serum without the use of a derivatization agent. We report an isotope dilution LC-MS/MS method, with electrospray ionization in the positive mode, that can simultaneously detect 24R,25(OH)2D3, 25(OH)D3, 3-epi-25(OH)D3, and 25-hydroxyvitamin D2. The method employs a cost-effective liquid-liquid extraction using only 150μL of sera and a total run time of 10min. Method performance was assessed by using quality controls made from pooled sera as an alternative to sera spiked with analytes. Biobanked samples, originally analyzed by chemiluminescent microparticle immunoassay (CMIA), were re-analyzed with this method to determine the contribution of 24R,25(OH)2D3 cross-reactivity to 25(OH)D measurement bias. The CMIA over-estimation of 25(OH)D measurements relative to LC-MS/MS was found to depend on both 25(OH)D and 24R,25(OH)2D3 concentrations.

  20. 流动注射-Luminol/Tween20化学发光体系测定邻苯二酚%Determination of catechol with flow injection-luminol/Tween 20 chemiluminescence system

    Institute of Scientific and Technical Information of China (English)

    谢建新; 伍贤学

    2013-01-01

    基于邻苯二酚对luminol-Tween 20化学发光体系的强烈的抑制作用,建立了一种简单、灵敏的luminol-Tween 20化学发光体系测定邻苯二酚的新方法.在最佳实验条件下,方法的检出限为5.0 ×l0-10mo1/L,线性范围为1.0 × 10-9mol/L~1.0×10-2mol/L,对5.0×10-8mol/L邻苯二酚进行平行测定11次,其RSD为4.1%.该方法可应用于自来水样中邻苯二酚的测定.%A simple and sensitive flow injection-chemiluminescence (CL) system of luminol-Tween 20 for the direct determination of catechol was developed.Proposed method was based on the strongly inhibition effect of catechol on the CL intensity of luminol-Tween 20 system.Under the optimial conditions,catechol was determined.The detection limit of the method is 5.0 × 10-10mol/L for catechol,the linear range for catechol is 1.0 × 10-9mol/L ~ 1.0 × 10-7mol/L.The relative standard deviation is 4.1% for 5.0 × 10-8mol/L catechol in 11 repeated measurements.The method was successfully applied to the detection of catechol in tap water samples.

  1. A simplified screening procedure for determination of total N-NO groups (TNG) and nitrite (NO2-) in commercial low-molecular-weight heparins (LMWH) by selective chemical denitrosation followed by high-sensitivity chemiluminescence detection (NO-analyzer, NOA).

    Science.gov (United States)

    Beretta, Giangiacomo; Gelmini, Fabrizio; Merlino, Mario; Furlanetto, Sandra; Facino, Roberto Maffei

    2009-07-12

    Aim of this work was to set up a method for the sensitive and selective determination of nitrite (NO(2)(-)) and total N-nitroso groups (TNG) in dalteparin and nadroparin, commercial low- molecular-weight heparins (LMWH), prepared by deaminative depolymerization of heparin with nitrous acid. The European Pharmacopoeia VI ed. indicates respectively 5 ppm as the maximum content for contaminant NO(2)(-) in the former and 0.25 ppm for TNG in the latter and no clear indication is given for N-NO groups in dalteparin, i.e. TNG must be absent because of the specific manufacturing process. The proposed technique is based on the development of a pre-analytical device, coupled to a chemiluminometer, constituted by three sequentially connected and commercially available purge vessels, where selective reagents are employed for the conversion of NO(2)(-) and N-NO to nitric oxide (NO). In detail, NO(2)(-) was determined in the first chamber and non-volatile and volatile TNG in the second and third. This method was validated for selectivity, sensitivity, linearity, accuracy and precision. The method was shown to be selective, with a quantitative linear range of 1-1000 ppb). The bias, intra- and inter-day percent relative error was lower than 1%. The contamination of NO(2)(-) and TNG in nadreparin was below the limits; for dalteparin NO(2)(-) fell within the limit, but there was a huge amount of TNG (15.80+/-0.05 ppm-6.69+/-0.02 ppm). Preliminary investigation on the solvent-extractable material from dalteparin showed the majority of chemiluminescence retained in the aqueous residue to indicate that this N-NO groups may belong to solvent unextractable material or be tightly bound to the dalteparin backbone.

  2. 化学发光法免疫测定对梅毒的诊断价值分析%Diagnostic Value of Chemiluminescence Immunoassay in the Diagnosis of Syphilis

    Institute of Scientific and Technical Information of China (English)

    王静

    2016-01-01

    Objective We evaluate the diagnostic value of that the chemiluminescence immunoassay (CLIA) test treponemal pallidum-specific antibody . Methods Serum of 739 patients were tested via CLIA and treponemal Hemagglutination (TPHA). Results The sensitivity of CLIA IS 98.85%and the specificity is 93.55,the diagnostic ratio is 1386.29,the positive predictive value is 99.56%, the negative predictive value is 85.96%,the prevalence rat is 92.96%,the positive likelihood ratio is 15.33, the negative likelihood ratio is 0.01. Conclusion The sensitivity and specificity of CLIA is high, and it can be widely used in clinical practice as a screening test for treponema pallidum-specific antibody.%目的:评估梅毒在化学发光免疫测定(CLIA)对梅毒的诊断价值。方法739例梅毒患者,进行CLIA试验,用TP血球凝集试验(TPHA)进行确诊。结果化学发光法检测梅毒特异性抗体的敏感度为:98.85%,特异度为:93.55%;诊断比值比为:1386.29;阳性预测值:99.56%;阴性预测值:85.96%;患病率:92.96%;阳性似然比:15.33;阴性似然比:0.01。结论 CLIA的灵敏度和特异度较高,可以作为梅毒特异性抗体筛查实验在临床上广泛应用。

  3. 化学发光酶免疫法测牛奶中3种喹诺酮类药物%Determination of QNs Residues in Milk by Enhanced Chemiluminescent Enzyme Immunoassay

    Institute of Scientific and Technical Information of China (English)

    李源珍; 生威; 刘恩梅; 韩静; 秦沛; 王硕

    2013-01-01

    A direct competitive enhanced chemiluminescent enzyme immunoassay(dc-CLEIA) for determination of ofloxacin (OFL), marbofloxacin (MAR), flerofloxacin (FLE) residues in milk was developed. The detection limit of assay were 0.01, 0.03, 0.04 ng/mL respectively. The spiked milk samples were extracted with trichloroacetic acid and analyzed by dc-CLEIA. The average recoveries at five spiked levels of 200, 100, 50, 20, 10 ng/mL were between 75.4%and 94.1%. There was a good correlation between data obtained using the dc-CLEIA and HPLC, indicating the good performance of this dc-CLEIA. Therefore, the proposed method was simple, accurateand and suitable for the rapid determination of ofloxacin, marbofloxacin and flerofloxacin in milk samples.%建立可同时检测牛奶中氧氟沙星(OFL)、麻保沙星(MAR)、氟罗沙星(FLE)残留的直接竞争化学发光酶免疫法(dc-CLEIA),方法的检出限分别为0.01、0.03、0.04 ng/mL。牛奶中的氧氟沙星、麻保沙星、氟罗沙星用7.5%的三氯乙酸提取,测定200、100、50、20、10 ng/mL 5个添加水平的回收率,平均回收率在75.4%~94.1%之间。HPLC与dc-CLEIA测定的结果有很好的相关性,说明所建立的dc-CLEIA可用于实际样品的检测,结果准确可靠。

  4. 偶合反应流动注射化学发光法测定扑热息痛%Flow injection chemiluminescence determination of paracetamol with chromium (VI)-H202-luminol system

    Institute of Scientific and Technical Information of China (English)

    王书民; 樊雪梅; 苏智魁; 陈凤英; 刘萍

    2011-01-01

    As chromium(Ⅵ) can be reduced by paracetamol to chromium(Ⅲ) which enhance the CL intensity of luminol-H2O2system, a new chemiluminescent method for the determination of paracetamol was developed. The relative CL intensity was linearly related to the concentration of paracetamol in the range of 4. 0 × 10-9 ~4. 0 ×10-5mol/L with a detection limit of 1.0 × 10-9mol/L. The relative standard deviation( n = 8) for 4. 0 × 10-7mol/L paracetamol was 2. 7%. The method has been applied to the determination of paracetamol in the tablets.%基于扑热息痛还原铬(Ⅵ)和铬(Ⅲ)催化鲁米诺-过氧化氢的化学发光,建立了氧化还原偶合反应流动注射化学发光法测定扑热息痛的新方法.方法线性范围为4.0×10-9~4.0×10-5mol/L,检出限为1.0×10-9 mol/L.对4.0×10-7 mol/L扑热息痛平行测定8次,其标准偏差为2.7%.已将该方法用于片剂中扑热息痛含量测定.

  5. 环境水样中碘的流动注射化学发光分析方法%A Novel Flow Injection Chemiluminescence Method for Iodide Determination in Environmental Water Samples

    Institute of Scientific and Technical Information of China (English)

    刘杨

    2012-01-01

    在酸性条件下,高锰酸钾氧化吡罗红B产生化学发光,I-的加入对该体系的化学发光有显著的增强作用,据此建立了流动注射化学发光法测定环境水样中I-的新方法.在优化的实验条件下,I-的质量浓度在8.0×10-4~1.0×10-1 μg/mL范围内与发光强度呈良好的线性关系,检出限为1.6×10-4 μg/mL.对0.1 μg/mLI-标准溶液进行11次平行测定,相对标准偏差为0.87%.将本法应用于环境水样中I-的测定,取得满意结果.%A new flow injection chemiluminescence (CL) method for the determination of iodide is described, which is based on the enhancement effect of iodide on the weak emission produced by potassium permanganate oxidation on pyronine B in acidic media. In the optimum conditions, CL intensities are proportional to the concentrations of iodide over the range 8. 0×10-4~l. 0×10-1 μg/mL with a detection limit of 1. 6×10-4 μg/mL. The relative standard deviation (RSD) is 0.87 % for 0. 1 jig/mL iodide (n=ll). The method has been applied to the determination of iodide in water samples with satisfactory results.

  6. [Comparison of antibody responses to hepatitis B surface antigen among four recipient groups of hepatitis B vaccines that have been approved in Japan: evaluation using passive hemagglutination assay and chemiluminescent immunoassay].

    Science.gov (United States)

    Ogata, Norio

    2009-10-01

    In hepatitis B virus (HBV) infection-preventing programs, serum or plasma levels of antibody to hepatitis B surface antigen (anti-HBs) are important to determine whether individuals are protective or not. We compared anti-HBs responses using passive hemagglutination assay (Mycell) and chemiluminescent immunoassay (Architect) among four recipient groups of HB vaccines, Meinyu, HBY, Bimmugen and Heptavax II, that have been approved in Japan. Overall, in a total of 1875 vaccinees Mycell results showed recipient groups of Meinyu and HBY acquired higher anti-HBs levels than those of Bimmugen and Heptavax II. Comparison of anti-HBs responses by both Mycell and Architect in recipient groups of Meinyu (n=150), HBY (n=218), Bimmugen (n=260), and Heptavax II (n=47) demonstrated the order of vaccinees' responses, such as geometric mean titers, ratios of acquiring high antibody levels (Mycell titers over 1024, Architect measurements over 1000 mIU/mL), and ratios of having unsuccessful antibody responses (Mycell titers under 8, Architect measurements under 10 mIU/mL), were somewhat different between the two assays. Comparison of Architect measurements at given Mycell titers revealed Bimmugen-recipients showed significantly lower values than HBY- or Heptavax II-recipients. Around critical protective levels, 5 of 22 Bimmugen-recipients with Mycell titers 16 or 32 showed Architect measurements under 10 mIU/mL, while 8 of 11 Heptavax II-recipients with Mycell titers below 8 demonstrated Architect measurements over 10 mIU/mL. Thus, discrepancies in anti-HBs evaluation between Mycell and Architect seemed to partly depend on administered vaccines. These results indicate anti-HBs concentration should be evaluated carefully so that we could completely prevent HBV infection.

  7. Development of an analytical method for the determination of beta2-agonist residues in animal tissues by high-performance liquid chromatography with on-line electrogenerated [Cu(HIO6)2]5- -luminol chemiluminescence detection.

    Science.gov (United States)

    Zhang, Yantu; Zhang, Zhujun; Sun, Yonghua; Wei, Yue

    2007-06-27

    A novel method was developed for the simultaneous determination of beta2-agonist residues such as terbutaline, salbutamol, and clenbuterol by high-performance liquid chromatography (HPLC) coupled with chemiluminescence (CL) detection. The procedure was based on the enhancement effect of beta2-agonists on the CL reaction between luminol and the complex of trivalent copper and periodate ([Cu(HIO6)2]5-), which was on-line electrogenerated by constant current electrolysis. The HPLC separation used a Nucleosil RP-C18 column (250 mm x 4.6 mm i.d., 5 microm; pore size, 100 A) with a mobile phase consisting of 90% acetonitrile and 10% aqueous ammonium acetate (20 mmol L-1, pH 4.0) at a flow rate of 1.0 mL min-1. The effects of several parameters on the HPLC resolution and CL emission were studied systematically. Liver samples were hydrolyzed with beta-glucuronidase followed by a solid-phase extraction procedure using Waters OasisMCX cartridges. Under optimum conditions, the limits of detection at a signal-to-noise ratio of 3 ranged from 0.007 to 0.01 ng g-1 and the limits of quantification at a signal-to-noise ratio of 10 ranged from 0.023 to 0.033 ng g-1 for three beta2-agonists. The relative standard deviations (RSDs) of intra- and interday precision were below 4.5%. The average recoveries for beta2-agonists (spiked at the levels of 0.05-5.0 ng g-1) in pig liver ranged from 84 to 110%, and the RSDs of the quantitative results were from 1.6 to 7.2%. The proposed method was successfully applied to the determination of beta2-agonist residues in pig liver samples.

  8. KMnO4-甲醛化学发光体系测定对乙酰氨基酚%Potassium permanganate-formaldehyde chemiluminescence system for determination of acetaminophen

    Institute of Scientific and Technical Information of China (English)

    王书民; 樊雪梅; 杨喆; 陈凤英; 郑行望

    2012-01-01

    基于对乙酰氨基酚在酸性介质下对KMnO4-甲醛化学发光体系强烈的增敏作用,建立了对乙酰氨基酚的流动注射化学发光测定方法.在最佳测试条件下,方法对对乙酰氨基酚的检出限(S/N=3)为1.0×10-10 mol/L,线性范围为1.0×10-9~1.0×10-5 mol/L对乙酰氨基酚,相关系数R为0.9993;对1.0×10-6 mol/L的对乙酰氨基酚溶液平行测定11次,其RSD为1.1%.该方法可应用于药物中对乙酰氨基酚含量的测定.%A sensitive flow injection chemiluminescence (FL-CL) method for the determination of acetaminophen was developed. Trie method was based on that acetaminophen could inhibit the CL reaction of formaldehyde and potassium permanganate in hydrochloric acid. Under the optimized conditions, acetaminophen was determined. The detection limit of the method is 1. 0 × 10 -10 mol/L acetaminophen, the linear range for acetaminophen is 1. 0 ×l0-9-1.0 ×10-5mol/L. The relative standard deviation is 1.1% for 1. 0 × 10 -6mol/L acetaminophen in 11 replicate measurements. The method was successfully applied to the determination of acetaminophen in medicament samples.

  9. Determination of Paracetamol in Pharmaceuticals by Fe3+ -H2O2 -Dichlorofluorescein Chemiluminescence System%Fe3+-H2O2-二氯荧光素化学发光体系测定药物中的扑热息痛

    Institute of Scientific and Technical Information of China (English)

    樊雪梅; 王书民; 尹治民; 苏智魁; 陈凤英; 王宪生; 崔凤灵

    2012-01-01

    A novel flow-injection chemiluminescence method was developed for the determination of paracetamol, which was based on the chemiluminescence reaction between paracetamol and hydroxy free radical produced by Fe3+ and H2O2 solution in the presence of dichlorofluorescein as energy transfer in the chemiluminescence system. Various factors influencing the chemiluminescence system were investigated and the possible mechanism was also discussed. Under the optimum conditions, the linear range for the determination of paracetamol was 8. 0×10 -8 - 5. 0 ×10-5 mol/L with a detection limit(S/N=3) of 5.0 ×10-9 mol/L, the relative standard deviation ( RSD, n =9) was 2. 2% for 3. 5×10-6 mol/L paracetamol solution. The method was successfully applied in the determination of paracetamol in pharmaceutical sample.%酸性介质中,Fe3+催化H2O2分解生成羟基自由基,进而氧化扑热息痛产生微弱的化学发光,二氯荧光素对该发光强度有较强的增敏作用.研究了影响化学发光强度的各种因素,并探讨了其可能的发光机理.在最佳化学发光条件下,其化学发光强度与扑热息痛的浓度在8.0×10-8~5.0×10-5 mol/L范围内呈良好的线性关系,检出限为5.0×10-9 mol/L,对3.5 ×10-6 mol/L的扑热息痛平行测定9次,其相对标准偏差为2.2%.该法用于片剂中扑热息痛含量的测定,结果满意.

  10. 对硫磷化学发光酶联免疫吸附分析方法的建立和评价%Development of an Indirect Competitive Chemiluminescence Enzyme-linked Immunoassay for Parathion

    Institute of Scientific and Technical Information of China (English)

    邓浩; 孔德彬; 杨金易; 徐振林; 沈玉栋; 杨星星; 孙远明

    2013-01-01

    Polyclonal antibody (PcAb) against parathion was raised and used to develop an indirect competi?tive chemiluminescence enzyme-linked immunoassay (icCLEIA). The hapten was prepared from thiophosphor-yl chloride after a three-step substitution reaction. The hapten was coupled to bovine serum albumin (BSA) and ovalbumin(OVA) as immounogen and coating antigen respectively by active ester method. New Zealand rabbits were immunized by the immunogen to obtain anti-parathion polyclonal antibody. Several parameters that might affect icCLEIA performance were carefully optimized. Under the optimum conditions, the linear range of the developed icCLEIA was 0.24-15.83 μg/L, the IC50 was 1. 14 μg/L and the limit of detection was 0. 09 μg/L. The average recovery of parathion from spiked vegetables and water samples ranged from 74.6% to 121.0%. In conclusion, the icCLEIA is a practical method for trace detection of parathion in real samples.%建立了基于多克隆抗体的对硫磷间接竞争化学发光酶联免疫吸附分析方法(icCLEIA).以三氯硫磷为原料,经三步取代反应合成对硫磷半抗原,通过活泼酯法将半抗原分别与牛血清蛋白(BSA)和卵清蛋白(OVA)偶联,制备免疫抗原和包被抗原.经免疫新西兰大白兔,获得对硫磷抗血清.通过优化条件参数,建立了对硫磷的icCLEIA分析方法.本方法的检测线性范围为0.24~ 15.83 μg/L;半抑制浓度IC50为1.14 μg/L;检出限为0.09 μg/L;对蔬菜样品和水样品的平均添加回收率为74.6%~121.0%.本方法可用于实际样品中痕量对硫磷残留检测.

  11. FI-Chemiluminescence Determination of Cocaine Hydrochloride in Microemulsion Medium%流动注射化学发光法测定微乳液介质中盐酸可卡因

    Institute of Scientific and Technical Information of China (English)

    朱乾华

    2011-01-01

    在酸性条件下,盐酸可卡因分子中氮原子被质子化后与阴离子AuCl4-形成离子缔合物,该缔合物被二氯甲烷带入含鲁米诺的氯化十六烷基三甲基铵反胶柬微乳液中,离解出来的AuCl4-立即与鲁米诺产生化学发光,结果表明:发光强度与盐酸可卡因的质量浓度在0.001~10mg·L-1范围内呈线性关系,据此提出了间接测定盐酸可卡因的微乳液介质一流动注射化学发光法。方法的检出限(3σ)为0.01μg·L-1。以尿样和血清样品为基体,用标准加入法测定方法的回收率,测得回收率在95.0%~102.0%之间,相对标准偏差(n=5)在1.8%~3.2%之间。%It was found that in an acidic medium, an ion association complex was formed between AuCl4- and the protonated nitrogen atom of cocaine hydrochloride, and this complex was extracted with dichloromethane and the extract was transfered into a reversed micellar microemulsion prepared by mixing cetyltrimethylammonium chloride (CTAC) and luminol. Then AuCL was released and reacted with luminol to produce chemiluminescence. Based on these facts, a FI-CL method for indirect determination of cocaine hydrochloride was proposed. Linearity range of the working curve was kept between 0. 001-- 10 mg·L-1 , with detection limit (3σ) of 0. 01 μg·L-1. Test for recovery was made by standard addition method using samples of urine and blood serum as matrixes, results of recovery found were in the range of 95.0%0--102.0% with RSD's (n=5) in the range of 1.8%-3.2%.

  12. CMIA法测定梅毒螺旋体抗体在临床筛检梅毒的应用%Evaluation of a chemiluminescent microparticle immunoassay for determination of Treponema pallidum antibodies in syphilis screening test

    Institute of Scientific and Technical Information of China (English)

    金国江; 邹大伟; 杜毅鑫; 陈俊丽

    2012-01-01

    目的:探讨CMIA法测定梅毒螺旋体抗体在临床筛检梅毒的应用.方法:用CMIA法测定患者梅毒螺旋体抗体,阳性标本再进行TPPA确认试验,用ROC曲线分析确定S/CO值筛检梅毒精确度.结果:在28468例受检患者中,CMIA法梅毒抗体阳性501例,其中TPPA阳性413例,通过ROC曲线分析确定S/CO为3.2时(曲线下面积0.953,95%可信区间0.936~0.970),预测梅毒精确度为(敏感性88.8%,特异性94.4%).结论:CMIA法检测梅毒抗体可以准确预测梅毒感染,根据S/CO值可以确定是否需要进行TPPA确认试验.%Objective: To explore the clinical application of a chemiluminescent microparticle immunoassay (CMIA) for determination of Treponema pallidum antibodies in syphilis screening test. Methods: All serum samples were detected for the Treponema pallidum antibodies by CMIA. Then the positive samples were confirmed by Treponema pallidum particle agglutination ( TPPA). Receiver - operating characteristic ( ROC ) curve analysis was performed to evaluate the diagnostic accuracy of Treponema pallidum antibody S/CO for syphilis diagnosis. Results: In total of 28,468 patients, 501 patients were CMIA positive, and 413 cases were TPPA positive. By ROC curve a-nalysis, Treponema pallidum antibody S/CO ratio ( area, 0. 953 ; 95% confidence interval, 0. 936 to 0. 970) accurately predicted the presence of Syphilis, with a cutoff value of 3. 2 ( sensitivity, 88. 8% ; specificity, 94. 4% ) . Conclusion: The CMIA for determination of Treponema pallidum antibodies can predict syphilis infection accurately. The S/CO ratio can be used to determine whether a TPPA test should be performed.

  13. 化学发光检测梅毒特异性抗体反应性结果分析%Reactive results of Treponema pallidum antibody in chemiluminescent assay

    Institute of Scientific and Technical Information of China (English)

    顾春瑜; 刘刚; 赵乔妹; 李少增; 于勇

    2011-01-01

    Objective To find the cause of false positive results during the detection of Treponema pallidum antibody(TP) in chemiluminescent assay. Methods We put 129 TP reactive samples to chemiluminscent assay, reexamined them by treponema pallidum hemaglutination assay (TPHA) before comparing them. Results When cut - off S/CO was 1, the difference was significant, but there was no significant difference when cut - off S/CO was 5. Conclusions When anti - TP is reactive, especially in the samples of low S/CO value(1.0 -5.0), multiple factors should be taken into consideration, such as age, history of disease, and clinical symptoms , followed by re - examination of the sample by other means. In this way, the chance of false positivity will be reduced.%目的 分析梅毒特异性抗体(treponema pallidum antibody,TP)的有反应性结果,尤其是COI值较低的标本,为减少假阳性的发生提供依据.方法 选取129例用化学发光法检测梅毒特异性抗体有反应性的术前筛查标本,用梅毒螺旋体血凝试剂盒(treponema pallidum hemaglutination assay,TPHA)进行复检,分析比对两者的结果.结果 当切点S/CO值为5时,Syphilis TP阳性为96例,阴性为33例,两种方法差异无统计学意义(P>0.05).结论 当TP有反应性时(尤其是S/CO值为1.0 ~5.0的标本),要综合考虑患者的年龄、病史,以及临床表现,可再用TPHA做复检,如两者均为阳性再报阳性,这样大大减少了假阳性的发生,降低了医疗纠纷的可能.

  14. Development of Chemiluminescence Enzyme Immunoassay for the Determination of Ochratoxin A in Cereal%谷物中赭曲霉毒素A化学发光酶免疫分析法的建立

    Institute of Scientific and Technical Information of China (English)

    刘星; 许杨; 何庆华

    2011-01-01

    建立了间接竞争化学发光酶免疫法检测赭曲霉毒素A(ochratoxin A,OTA),该方法IC50为112 pg/mL,检出限是2.47 pg/mL,平均批内和批间变异系数分别为7.03%和14.7%.在大米和小麦样本中添加浓度1.5~6 μg/kg的OTA标品,平均回收率在66.97%~97.96%之间,与其他常见真菌毒素未见交叉反应.将该方法应用于30份谷物样本(包括20份大米样本和10份小麦样本)中OTA的检测,检测结果与商品化ELISA试剂盒的相关系数R2= 0.942 4.该方法简单、灵敏、快速、准确适用于谷物中OTA的检测.%A sensitive indirect competitive chemiluminescence enzyme immunoassay (CLEIA)for OTA was developed. The concentration of OTA causing 50% inhibition of binding enzyme marker (IC50) was 112 pg/mL and the detection limit was 2.47 pg/mL, the mean coefficients of variation of within groups and between groups were 7.03,14.7% respectively. The recoveries from rice and wheat samples spiked with 1.5 ~ 6 Ig/kg of OTA varied between 66.97% and 97.96% ,and the OTA antibody showed no cross -reactivity to the else common mycotoxins. OTA in 30 cereal samples were screened by the CLEIA, including 20 rice samples and 10 wheat samples. The results show good coefficient with commercial ELISA kit (R2 = 0. 9424). The assay was sensitive, fast and accurate, which proved to be suitable for the screening of cereal samples for the presence of OTA.

  15. Sequential injection analysis (SIA)-chemiluminescence determination of indomethacin using tris[(2,2'-bipyridyl)]ruthenium(III) as reagent and its application to semisolid pharmaceutical dosage forms

    Energy Technology Data Exchange (ETDEWEB)

    Mervartova, Katerina [Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, CZ-500 05 Hradec Kralove (Czech Republic)], E-mail: Katerina.Mervartova@faf.cuni.cz; Polasek, Miroslav [Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, CZ-500 05 Hradec Kralove (Czech Republic); Calatayud, Jose Martinez [Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, Valencia (Spain)

    2007-09-26

    Automated sequential injection (SIA) method for chemiluminescence (CL) determination of nonsteroidal anti-inflammatory drug indomethacin (I) was devised. The CL radiation was emitted in the reaction of I (dissolved in aqueous 50% v/v ethanol) with intermediate reagent tris(2,2'-bipyridyl)ruthenium(III) (Ru(bipy){sub 3}{sup 3+}) in the presence of acetate. The Ru(bipy){sub 3}{sup 3+} was generated on-line in the SIA system by the oxidation of 0.5 mM tris(2,2'-bipyridyl)ruthenium(II) (Ru(bipy){sub 3}{sup 2+}) with Ce(IV) ammonium sulphate in diluted sulphuric acid. The optimum sequence, concentrations, and aspirated volumes of reactant zones were: 15 mM Ce(IV) in 50 mM sulphuric acid 41 {mu}L, 0.5 mM Ru(bipy){sub 3}{sup 2+} 30 {mu}L, 0.4 M Na acetate 16 {mu}L and I sample 15 {mu}L; the flow rates were 60 {mu}L s{sup -1} for the aspiration into the holding coil and 100 {mu}L s{sup -1} for detection. Calibration curve relating the intensity of CL (peak height of the transient CL signal) to concentration of I was curvilinear (second order polynomial) for 0.1-50 {mu}M I (r = 0.9997; n = 9) with rectilinear section in the range 0.1-10 {mu}M I (r = 0.9995; n = 5). The limit of detection (3{sigma}) was 0.05 {mu}M I. Repeatability of peak heights (R.S.D., n = 10) ranged between 2.4% (0.5 {mu}M I) and 2.0% (7 {mu}M I). Sample throughput was 180 h{sup -1}. The method was applied to determination of 1 to 5% of I in semisolid dosage forms (gels and ointments). The results compared well with those of UV spectrophotometric method.

  16. Serological Thymidine Kinase 1 is a Biomarker for Early Detection of Tumours—A Health Screening Study on 35,365 People, Using a Sensitive Chemiluminescent Dot Blot Assay

    Directory of Open Access Journals (Sweden)

    Ellen He

    2011-11-01

    Full Text Available Serological thymidine kinase 1 (STK1 is a reliable proliferation marker for prognosis, monitoring tumour therapy, and relapse. Here we investigated the use of STK1 in health screening for early detection of pre-malignant and malignant diseases. The investigation was based on 35,365 participants in four independent health screening studies in China between 2005–2011. All participants were clinically examined. The concentration of STK1 was determined by a sensitive chemiluminescent dot blot ECL assay. The ROCvalue of the STK1 assay was 0.96. At a cut-off STK1 value of 2.0 pM, the likelihood (+ value was 236.5, and the sensitivity and the specificity were 0.78 and 0.99, respectively. The relative number of city-dwelling people with elevated STK1 values (≥2.0 pM was 0.8% (198/26,484, while the corresponding value for the group of oil-field workers was 5.8% (514/8,355. The latter group expressed significantly higher frequency of refractory anaemia, fatty liver, and obesity, compared to the city dwellers, but no cases of breast hyperplasia or prostate hyperplasia. Furthermore, people working in oil drilling/oil transportation showed higher STK1 values and higher frequency of pre-malignancies and benign diseases than people working in the oil-field administration. In the STK1 elevated group of the city-dwelling people, a statistically significantly higher number of people were found to have malignancies, pre-malignancies of all types, moderate/severe type of hyperplasia of breast or prostate, or refractory anaemia, or to be at high risk for hepatitis B, compared to people with normal STK1 values (< 2.0 pM. No malignancies were found in the normal STK1 group. In the elevated STK1 group 85.4% showed diseases linked to a higher risk for pre-/early cancerous progression, compared to 52.4% of those with normal STK1 values. Among participants with elevated STK1 values, 8.8% developed new malignancies or progress in their pre-malignancies within 5 to 72

  17. Determination of Pb (Ⅱ) in drinking water by luminol-1,10-phenanthroline-H2O2 chemiluminescence system%鲁米诺-邻菲啰啉-过氧化氢化学发光体系检测饮用水中Pb(Ⅱ)

    Institute of Scientific and Technical Information of China (English)

    周敏; 滕久委; 燕廷; 唐书泽

    2011-01-01

    采用流动注射与化学发光法相结合,建立Pb(Ⅱ)催化过氧化氢氧化鲁米诺和邻菲啰琳的化学发光体系,用以检测饮用水中的Pb(Ⅱ).通过考察鲁米诺溶液浓度、过氧化氢溶液浓度、邻菲啰啉浓度、铅液pH值及缓冲液pH值等单因素,选定最初条件,通过正交试验选定最佳条件.结果表明,Pb(Ⅱ)对鲁米诺-邻菲啰啉-H2O2体系的发光强度有明显增强作用,在选定的最佳条件下,当Pb(Ⅱ)质量浓度为1.0×10-7~1.0 ×10-5g/mL时,Pb(Ⅱ)对鲁米诺-邻菲啰啉-H2O2体系发光强度的促进作用与Pb(Ⅱ)质量浓度呈现较好的线性关系,利用这一线性关系,可快速测定饮用水中Pb(Ⅱ)的浓度.%Catalytic reaction of luminol-phenanthroline-H2O2 chemiluminescence by Pb( Ⅱ ) was tested by combining flowing-injection and chemiluminescence.Through the single factor experiment about the concentration of luminous,hydrogen peroxide, 1, 10-phenanthroline, the pH value of Pb solution and buffer solutions, the initial was selected and the best condition was designated through orthogonal test.The results showed that Pb ( Ⅱ ) enhanced the chemiluminescence intensity of luminol-l,10-phenanthroline-H2O2, and the stimulation of Pb( Ⅱ ) had a positive correlation with its concentration in drinking water when its concentration ranged from 1.0×10-7 g/mL to 1.0 ×10-5 g/mL.It proved to be a rapid method to determine the sudden change of Pb ( Ⅱ ) in drinking water and to carry out the goalas an early alarming of emergency contamination ofPb ( Ⅱ ).

  18. Spectroscopic Analysis of PbO Chemiluminescence.

    Science.gov (United States)

    1981-12-01

    Steinfeld, Jeffrey. Molecules and Radiation. New York: MIT Press, 1978. 2. Herzberg , G. Spectra of Diatomic Molecules, (Second Edition): New York...18. SUPPLEMENTARY NOTES Aovel vot releasCe; IAW AFR 190-17 FREDERICK C. L NCH,kM jor, USAF Director of Public Af irs 19. KEY WORDS (Continue on

  19. 化学发光免疫分析法与酶联免疫法检测艾滋病抗体的对照研究%A contrastive study on chemiluminescence immunoassay and ELISA for detecting HIV antibody

    Institute of Scientific and Technical Information of China (English)

    程木好; 王业涌; 高广彬

    2016-01-01

    目的:对照研究化学发光免疫分析法(CLIA)与酶联免疫法(ELISA)检测艾滋病(HIV)抗体的效果,以便探究CLIA检测HIV抗体的可行性。方法于2013年2月至2015年3月随机抽查我市3542例艾滋病高危人员,采集血清,分别采用CLIA与ELISA法检测血清中的HIV抗体,并采用蛋白印迹检测法对检测结果进行确证。结果 ELISA法检测显示血清标本为阳性的56份,阳性率为1畅58%,而CLIA检测阳性61份,阳性率为1畅72%;3542份血清标本中,11份标本ELISA法与CLIA法检测结果不一致;CLIA法的阳性预测值为96畅72%,检测灵敏度为98畅33%,特异度为99畅94%;ELISA法的阳性预测值为96畅43%,检测灵敏度为90畅00%,特异度为99畅94%;ELISA法与CLIA法在阳性预测值、检测灵敏度、特异度等指标上无显著的差异性( P>0畅05)。结论 CLIA的灵敏度、特异度、准确性高,可用于HIV抗体检测。%Objective To compare the effects of Chemiluminescence immunoassay ( CLIA) and ELISA for detecting the HIV antibody in order to explore the feasibility of CLIA.Methods 3,542 AIDS high-risk people were randomly selected from February 2013 to March 2015 in our city, the serum was collected and HIV antibodies in the serum were detected by CLIA and ELISA.The detection results were confirmed by the Western blot.Results By ELISA assay, 56 serum samples were positive with a positive rate of 1.58%, while CLIA 61 were positive with a pos-itive rate of 1.72%.The detection results on 11 serum samples were inconsistent among the 3,542 serum samples by ELISA and CLIA.The positive predictive value of CLIA was 96.72%, the detection sensitivity 98.33%and specific-ity 99.94%, while the positive predictive value of ELISA was 96.43%, the detection sensitivity 90.00%and speci-ficity 99.94%.The positive predictive value, sensitivity, specificity and other indicators showed no significant differ-ence between

  20. Content Determination of Rutin in Rutin Tablets by Luminol-potassium Hypermanganate Chemiluminescence Method%鲁米诺-高锰酸钾化学发光法测定芦丁片中芦丁的含量

    Institute of Scientific and Technical Information of China (English)

    白瑞霞; 宋素异

    2013-01-01

    OBJECTIVE: To establish a novel, rapid and sensitive method for the determination of rutin. METHODS: Rutin was found to enhance the chemiluminescence (CL) signal of luminol-potassium hypermanganate system in alkaline medium. Based on flow injection (FI), a new FI-CL method was developed for the determination of rutin. The concentrations of reagents were lu-minol (2.0×10-5 mol/L), potassium hypermanganate (8.0xl0~5 mol/L), sodium hydrate (0.05 mol/L); the flow rates of pumps were set at 2.12 ml/min (P1) and 3.45 ml/min (P2) with negative high voltage of 600 V and sampling time of 15 s. RESULTS: The linear range of rutin was 2.0×l0-9-2.0×l0-7 g/ml (r=0.999 1)with average recovery of 101.57% (RSD=1.69,n=3). The detection limit was 8.3×l0-10 g/ml. CONCLUSIONS: The method is convenient, rapid and sensitive, and can provide a new method for the quality control and product detection of rutin.%目的:建立一种快速、灵敏地测定芦丁含量的新方法.方法:利用在碱性条件下,芦丁对鲁米诺-高锰酸钾体系有强烈的增强作用的原理,结合流动注射技术,建立了流动注射化学发光法检测芦丁的新方法;反应试剂的浓度分别为:鲁米诺(2.0×10-5mol/L)、高锰酸钾(8.0× 10-5 mol/L)、氢氧化钠溶液(0.05 mol/L),主泵(P1)、副泵(P2)流速分别为2.12、3.45 ml/min,光电倍增管负高压为600V,采样时间为15s.结果:芦丁的检测质量浓度线性范围为2.0×10-9~2.0×10-7 g/ml(r=0.999 1),检出限为8.3×10-10g/ml,平均加样回收率为101.57%,RSD= 1.69%(n=3).结论:该方法简单、快速、灵敏性高,为芦丁药物的质量控制和体内分析提供了一种新的检测方法.

  1. MnO_2纳米溶胶-甲醛化学发光体系及其分析应用研究%Chemiluminescence of Nano-Colloidal MnO2 with Formaldehyde and Its Analytical Application

    Institute of Scientific and Technical Information of China (English)

    杜建修; 王虹

    2012-01-01

    Water-soluble forms of colloidal MnO2 were prepared by the chemical reduction of KMnO4 with Na2S203 under neutral aqueous condition. The as-prepared colloidal MnO2 solution is dark-brown, trans- parent, stable, and possesses the maximum absorption peak at 375 nm and an average diameter of 40 nm. The as-prepared nano-colloidal MnO2 was found to react with formaldehyde to generate weak chemiluminescence (CL) under acidic condition. The effects of more than 30 pharmaceuticals on the nancolloidal MnO2-formaldehyde CL system were tested. Pharmaceuticals including phenothiazines and aminoethanethiols were observed to enhance the CL signal significantly. The experimental conditions were well optimized and the analytical figures for five phenothiazines and four aminoethanethiols were presented. The method was validated by the analysis of perphenazine in tablets and chlorpromazine hydrochloride in swine feed. The CL reaction mechanism was discussed by the study of CL spectra, fluorescence spectra, UV-vis absorption spectra, and other experiments. All of CL reactions had the same maximum emission wavelength about 640 nm, which suggested that the CL emitter was independent of analytes. The CL signal was inhibited obviously by single-state oxygen scavengers, sodium azide and 1,4-diazabicyclo[2,2,2]octane, indicating that single-state oxygen dimer was the potential CL emitter for the present CL reaction.%Na2S2O3在中性水溶液中还原KMnO4可制备得到暗棕色的可溶性MnO2溶胶.所制备的MnO2溶胶透明、稳定,最大吸收波长位于357 nm处,平均粒径约40 nm.研究发现,所制备的MnO2纳米溶胶在酸性介质中与甲醛反应可产生弱的化学发光.考察了近30种药物分子在MnO2纳米溶胶-甲醛体系中的化学发光行为.结果表明,吩噻嗪类药物、氨基硫醇类药物等对该体系的化学发光信号具有显著的增强作用.据此,建立了利用这一化学发光体系测定五种吩噻嗪类药物和四种氨基

  2. Silver nanoparticles enhanced luminol liquid chemiluminescence system for the determination of paracetamol by flow injection analysis%纳米银增敏鲁米诺液相化学发光分析法测定4-乙酰氨基酚的研究

    Institute of Scientific and Technical Information of China (English)

    杨猛; 马红燕; 辛建伟; 罗娟娟

    2011-01-01

    研究发现在碱性条件下,纳米银对鲁米诺-铁氰化钾液相化学发光体系发光信号具有明显的增敏作用,而4-乙酰氨基酚对该体系具有强烈的抑制作用。结合流动注射技术,建立了流动注射化学发光分析法测定对乙酰氨基酚的新方法。该方法测定对乙酰氨基酚的线性范围为9.0×10-12~1.0×10-10g/mL(0.9947)和1.0×10-10~7.0×10-9g/mL(0.9904),检出限(3σ)为7.9×10-12g/mL。对5.0×10-11g/mL和5.0×10-10g/mL对乙酰氨基酚平行测定11次,其相对标准偏差为1.3%和1.3%。该方法用于片剂中对乙酰氨基酚的测定,回收率为97.0%~101.5%。%The experiments indicated that silver nanoparticles ( AgNPs ) could greatly enhance the chemiluminescence intensity of the luminol-K3Fe(CN)6 system in sodium hydroxide medium, but paracetamol could inhibit the chemiluminescence signals significantly. On the basis of the inhibition, a novel flow-injection chemiluminescence method for the determination of paracetamol was proposed. The experimental conditions that influence the chemiluminescence intensity were carefully studied and optimized. Under the optimum conditions, the calibration graph for paracetamol was obtained. The linear range for the determination of paracetamol was from9 × 10T-12 to 1.0 × 10-10g/mL(0.9947) and 1.0 × 10-10 to 7.0 × 10-9G/mL(0. 9904) with a detection limit of 7.9 × 10-12g/mL. The relative standard deviations for 11 repeated measurements of 5.0 × 10-11 g/mL and 5.0 × 10-10 g/mL paracetamol were 1.3% and 1.3%, respectively. Possible interferences from major excipients in Pharmaceuticals and other related compounds were also studied. Contents of paracetamol in two locally available Pharmaceuticals were analyzed. The method has the merits of rapidness, simplicity, high sensitivity, wide linear range and has been used for the analysis of paracetamol in commercial tablets with recoveries in the range from 97.0% to 101.5%.

  3. 应用FITC系统建立非均衡竞争游离三碘甲腺原氨酸(FT3)化学发光法%Detection of free triiodothyronine in human serum by non-equilibrium competitive chemiluminescence immunoassay using FITC system

    Institute of Scientific and Technical Information of China (English)

    孔海霞; 刘萍; 田云霞; 宋启超; 王世鑫; 翁亚光

    2012-01-01

    目的 制备抗人T3多克隆抗体,应用异硫氰酸荧光素(fluorescein isothiocyanat,FITC)系统开发新型非均衡竞争技术,建立一种能广泛应用于临床检测游离三碘甲腺原氨酸(free triiodothyronine,FT3)的方法.方法 以T3-牛血清白蛋白(bovin serum albumin,BSA)为抗原免疫新西兰大白兔,制备抗人T3多克隆抗体,亲和层析法纯化并联接辣根过氧化物酶(horse radish peroxidase,HRP).用FITC标记T3-类似物.采用抗FITC抗体包被发光板,FITC-T3类似物与抗FITC抗体结合形成固相抗原,通过非均衡竞争法原理,固相抗原与血清中FT3竞争结合抗T3抗体-HRP,通过标准曲线计算FT3浓度,建立方法学评价并与直接用T3-牛血清γ球蛋白(Bovine serum gamma globulin,BGG)包被(非FITC系统)的检测系统相比较.将本方法应用于临床120份血清标本的检测,定量结果与德国罗氏2010电化学发光全自动免疫分析系统( Elecsys 2010)定量结果进行比较.结果 抗体经酶联免疫吸附实验证明对T3、具有高度特异性.应用FITC系统建立的非均衡竞争化学发光免疫分析标准曲线的线性R>0.99,线性范围0.25~50 pg/ml,分析灵敏度为0.25 pg/ml,精密度测试批内、批间变异系数CV<5%,检测结果优于非FITC系统的检测.对于临床血清标本的检测,其定量结果与德国Elecsys 2010定量试剂盒的定量结果具有很好的相关性,相关系数r =0.962 2,两者的测定值无显著性差异.结论 制备的抗人T3多克隆抗体特异性强,以FITC系统为平台建立的非均衡竞争FT3检测化学发光法具有精密性好、灵敏度高、稳定性强,有良好的准确性,达到临床测定的需求,可代替进口化学发光产品用于临床样本的检测.%Objective To prepare a polyclonal antibody ( mAb) against human triiodothyronine ( T3) and to establish a new method of non-equilibrium competitive chemiluminescence immunoassay ( CLIA) with FITC system for clinical

  4. Chemiluminescence Immunoassay for Quantitative Analysis of Prostate Specific Antigen Complexed toα1-Antichymotrypsin in Human Serum%化学发光免疫分析法检测血清中前列腺特异抗原复合物

    Institute of Scientific and Technical Information of China (English)

    周友俊; 李娇霞; 程绘珺; 杨乔芬; 何美琼; 郭丽萍; 邓智勇

    2016-01-01

    Eight mouse hybridoma cell lines which stably secreted monoclonal antibodies ( McAbs ) against human prostate-specific antigen-α1-antichymotrypsin complex ( PSA-ACT ) were obtained through hybridoma technique. After purification, the immunological characters of 8 McAbs were identified and classified by epitopes analysis through indirect enzyme-linked immunosorbent assay ( ELISA) . A pair of McAbs was chosen from above 8 McAbs, based on which a highly sensitive, simple and rapid chemiluminescence enzyme immunoassay ( CLEIA) was developed for determination of PSA-ACT in human serums using the lumino-H2 O2 reaction catalyzed by horseradish peroxidase ( HRP) as the chemiluminescence system. Several experiment factors such as coating buffer, coating concentration, dilution ratio of PSA-ACT-HRP complex, incubation time, immunoreaction protocol and chemiluminescence reaction time were optimized. The results showed that the linear range of the proposed method for PSA-ACT determination was 0-40 ng/mL (R2=0. 9943), with the detection limit of 0. 53 ng/mL. The inter-assay relative standard deviations (RSDs) were 4. 6%-6. 6%, and intra-assay RSDs were 5 . 7%-8 . 0%. The recoveries of PSA-ACT at three spiked levels in serum samples were 95. 4%-104. 2%. The proposed method exhibited a cross-reactivity of 0. 6% with free-PSA. The proposed method is stable, sensitive, rapid and simple, and provides a foundation for the development of PSA-ACT CLEIA kit and shows great value in clinical auxiliary diagnosis of prostate cancer.%采用杂交瘤技术构建抗前列腺特异抗原-α1-抗胰凝乳蛋白酶( Prostate specific antigen-α1-antichymot-rypsin complex,PSA-ACT)单克隆抗体(单抗)细胞株,共获得8株稳定分泌PSA-ACT单抗的细胞株,纯化后进行免疫特性鉴定并做单抗配对。选择其中一对单抗,采用辣根过氧化物酶( HRP)催化鲁米诺( lumino)-H2 O2化学发光体系,建立了测定人血清中PSA-ACT浓度的化

  5. Determination of Chromium(CrⅢ)in Drinking Water by Potassium Bromide-Luminol-H2O2 Chemiluminescence System with Flow Injection%流动注射溴化钾-鲁米诺-过氧化氢化学发光体系检测饮用水中铬(CrⅢ)

    Institute of Scientific and Technical Information of China (English)

    周敏; 燕廷; 滕久委; 唐书泽

    2011-01-01

    Determination of Chromium (CrⅢ) in Drinking Water by Potassium bromide- Luminol-H2O2 Chemiluminescence System with Flow InjectionAbstract: Based on the obvious sensitization of potassium bromide on the chemiluminescent reaction in the system of luminol-H2O2 - chromium (CrⅢ), a sensitive chemiluminescent method for determination of CfⅢ with flow injection technique was developed. The method is simple ,rapid and effective to determine CfⅢ with the range of ( 1 × 10-6 ~ 1 × 10 -3 ) mg/mL and detection limit of 2.06 × 10 -7 mg/mL. The relative standard deviation (RSD) is 2.39× for the determination of 2.0 × 10-6 mg/mL of Cr (Ⅲ) (n = 11 ). Ethylenediaminetetra acetic acid disodium salt (EDTA-2Na) is effectively eliminated the interference of some metal ions such as Fe2+ , Fe3+ , Cu2+ , Zn2+. Therefore, it is a rapid method for determination of CrⅢ sudden changes in drinking water and a good monitor in giving an early alarm in the emergency contamination by heavy metals. The recoverie of CrⅢ was 91.7% ~97.5%.%基于溴化钾对鲁米诺-过氧化氢-铬(CrⅢ)化学发光反应有较强的敏化作用,结合流动注射技术,建立了溴化钾-鲁米诺-过氧化氢-CrⅢ化学发光体系测定CrⅢ的分析方法.该法测定CrⅢ的线性范围为1×10-6~1×10-3mg/mL,检出限为2.06×10-7mg/mL.对浓度2.0×10-6mg/mL CrⅢ标准溶液平行测定11次,相对标准偏差(RSD)为2.39%.利用乙二胺四乙酸二钠(EDTA-2Na)作掩蔽剂,可有效地消除水体Fe2+、Fe3+、Cu2+、Ca2+、Zn2+等金属离子对测定CrⅢ的干扰,从而快速测定饮用水中CrⅢ的突然变化,达到对饮用水重金属突发性污染早期快速预警的目的,应用该方法,CrⅢ检测回收率达到91.7%~97.5%.

  6. 人血清CYFRA21-1化学发光免疫分析方法的初步建立及应用%The Preliminary Establishment and Application of Chemiluminescence Immunoassay for Detection of Human CYFRA21 -1 in Serum

    Institute of Scientific and Technical Information of China (English)

    潘慧杰; 刘红; 唐磊; 李嘉; 杨萌萌; 杨铁生; 樊春红; 杨晓林; 孙旭东

    2012-01-01

    Objective To estimate an enhanced chemiluminescence immunoassay for detection CYFRA21-1 in serum.Methods CYFRA21-1 chemiluminescence quantitative immunoassay kit was used.308 clinical samples were detected, compared with ECLA CY-FRA21-1 immunoassay.Results The sensitivity of the assay was 0.04μg/L.The linear range was (1.5 ~ ICO) μg/L, and there was no significant cross-reaction with NSE or CEA.The recovery rate of addition and dilution were both below 10% .Besides, the intra- and inter-coefficients of variations were from 90% to 110% .The 0.95 confidence interval for normal samples was 0 ~3.4μg/L (n =231) .Comparing to ECLA, the clinical accordance rate was 96.65% .Conclusion The sensitivity, specificity, stability and detection range of this method are all good enough to meet the clinical requirement, furthermore, to take the place of the foreign like products.%目的:建立人血清CYFRA21-1化学发光免疫分析方法并在临床检测中的应用.方法:使用CYFRA21-1定量测定试剂盒(化学发光法)检测308例临床血清标本,并与CYFRA21-1电化学发光全自动免疫分析方法进行对比.结果:灵敏度0.04μg/L,线性范围(1.5~100)μg/L,与NSE及CEA无交叉反应,样本中抗凝剂量的柠檬酸钠、肝素、EDTA-Na2对测定结果没有影响.添加回收率和稀释回收率为90%~110%,分析内和分析间变异系数均<10.0%.该方法正常参考值为(0~3.4)μg/L(95%可信限).ECLA测定结果与本法相比,临床总符合率为96.65%.结论:该方法灵敏度高、特异性好、稳定性强,检测范围宽,有良好的准确性和重复性,完全可替代进口化学发光试剂用于临床样本的检测.

  7. Evaluation of the Architect Epstein-Barr Virus (EBV) viral capsid antigen (VCA) IgG, VCA IgM, and EBV nuclear antigen 1 IgG chemiluminescent immunoassays for detection of EBV antibodies and categorization of EBV infection status using immunofluorescence assays as the reference method.

    Science.gov (United States)

    Corrales, Isabel; Giménez, Estela; Navarro, David

    2014-05-01

    Commercial immunoassays for detecting IgG and IgM antibodies against Epstein-Barr virus (EBV), viral capsid antigens (VCA), and IgGs toward EBV nuclear antigen-1 (EBNA-1) are routinely used in combination to categorize EBV infection status. In this study, we evaluated the performances of the Architect EBV VCA IgG, VCA IgM, and EBNA-1 IgG chemiluminescent microparticle assays (CMIAs) in EBV serological analyses using indirect immunofluorescence assays and anticomplement immunofluorescence assays as the reference methods for VCA IgG, VCA IgM, and EBNA-1 IgG antibody detection, respectively. A total of 365 serum samples representing different EBV serological profiles were included in this study. The κ values (concordances between the results) obtained in the Architect CMIA and those in the reference assays were 0.905 (P EBV infection, and 92.42% and 97.82% for diagnosing the absence of an EBV infection. In summary, we demonstrated that the Architect EBV antibody panel performs very well for EBV antibody detection and correctly categorizes clinically relevant EBV infection states.

  8. Validation of the extension of the range of application and of the single system of injection for the determination of total nitrogen in petroleum and its derivatives by chemiluminescence; Validacao da ampliacao da faixa de aplicacao e do sistema unico de injecao para a determinacao de nitrogenio total em petroleos e derivados por quimiluminescencia

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Maria de Fatima Pereira dos [Fundacao Gorceix, Ouro Preto, MG(Brazil)]. E-mail: santos@cenpes.petrobras.com.br; Tamanqueira, Juliana Boechat [Universidade Federal Fluminense, Niteroi, RJ (Brazil); Magalhaes, Julio Cesar Dias [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas. Avaliacao de Petroleo; Oliveira, Elcio Cruz de [Transpetro, Rio de Janeiro, RJ (Brazil). Logistica e Planejamento; Vaitsman, Delmo Santiago [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Quimica. Dept. de Quimica Analitica

    2003-12-01

    With the objectives of using a single system of injection and of extending the range of application for the method ASTM D4629/02 'Total Nitrogen in Petroleum Derivatives by the System of Injection with a Syringe and Detection by Chemo- Luminescence', it was studied a procedure by statistical validation with the objective of guaranteeing the analytical reliability of the assay and allowing the inclusion of samples of petroleum and heavy derivatives in one single methodology. The determination of total nitrogen for petroleum and derivatives is traditionally assayed using the method of Kjeldahl - a time-consuming methodology that requires a large amount of time for giving the final result, at the same time that is not recommended for concentrations below 0.1%w/w, which does not meet the requirements for the specifications of the product, in the petroleum industry. An alternative for the method of Kjeldahl is the pyro-chemo luminescence, which allows the achievement of more repetitive results for total nitrogen. In the detection of nitrogen, the technique combines the reliability of oxidative combustion with the sensitivity of chemiluminescence. Therefore, it was developed a protocol of validation in the methodology ASTM D4629/02 for the validation of the extension of the range of application and for the evaluation of the performance of the equipment in analytical conditions, according to the calibration curve. (author)

  9. 碳量子点对基于能量转移电化学发光DNA传感体系影响的研究%Study on the Influence of Carbon Quantum Dots on Electrogenerated Chemiluminescence Energy Transfer Sensing System of DNA

    Institute of Scientific and Technical Information of China (English)

    杨帆; 王伶俐; 郭志慧

    2012-01-01

    The effect of carbon quantum dots on the electrogenerated chemiluminescence (ECL) energy transfer sensing system for the determination of DNA was investigated. The results showed that the signal-to-noise ratio of luminol-crystal violet ECL system was increased and its reproducibility was improved by the addition of carbon quantum dots. The conditions of the detection of DNA were optimized. Under optimal conditions, the linear range for the determination of target DNA was 1.0× 10^-11 -1.0× 10^-7 mol/L, the correlation coefficient was 0.9881 and the detection limit was 4.0 × 10^-12 mol/L. The possible interaction mechanism between DNA and carbon quantum dots was also discussed. This method was convenient and showed high sensitivity.%实验发现在鲁米诺-结晶紫能量转移电化学发光体系中再加入碳量子点后,体系的灵敏度和重现性均提高.本实验中,探究出用能量转移电化学发光分析法区分单双链DNA的优化条件.在优化条件下,测定目标DNA的线性范围为1.0×10-11~1.0×10-7mol/L,相关系数为0.9881,检出限为4.0×10-12mol/L,并且讨论了DNA与碳量子点可能的作用方式.本法操作方便,灵敏度高.

  10. Comportamento do método quimioluminescente-ELISA em relação a resultados considerados discordantes por meio de três técnicas convencionais para diagnóstico da doença de Chagas Behavior of the chemiluminescent ELISA method in relation to results considered discordant via three conventional techniques for diagnosing Chagas disease

    Directory of Open Access Journals (Sweden)

    Cláudia Regina De Marchi

    2007-02-01

    Full Text Available Quando utilizadas, em conjunto, a hemaglutinação indireta, a imunofluorescência indireta e ELISA para diagnóstico sorológico da doença de Chagas por vezes ocorrem resultados considerados discordantes, por não haver concordância entre o que indicam essas técnicas. A disponibilidade do método quimioluminescente-ELISA permitiu executá-lo com 200 soros que examinados pelos três testes citados que motivaram a obtenção de resultados discordantes. Com o método quimioluminescente-ELISA sucederam 193 negativos e sete positivos. O emprego desse novo procedimento trouxe mais um subsídio para compreensão do assunto, mas avanço mais concreto dependerá de documentação com soros de pessoas infectadas ou não pelo Trypanosoma cruzi conforme comprovação parasitológica.When indirect hemagglutination, indirect immunofluorescence and enzyme-linked immunosorbent assay are used together for serologically diagnosing Chagas disease, results that are considered discordant sometimes occur because there is disagreement between what these tests indicate. The availability of the chemiluminescent ELISA method enabled tests on 200 serum samples that had previously produced discordant results from the three abovementioned methods. CL-ELISA revealed that 193 of these samples were negative and seven were positive. The use of this new procedure provides further support for understanding this subject, but more concrete advances will depend on documentation with blood analyses from people previously demonstrated to be unquestionably infected or uninfected with Trypanosoma cruzi.

  11. Development of an indirect competitive chemiluminescence immunoassay for determining ractopamine residues in swine meat and urine%检测猪尿、猪肉中莱克多巴胺的化学发光间接竞争免疫分析方法的建立

    Institute of Scientific and Technical Information of China (English)

    王权; 赵新宇; 蒋蔚; 陈永军; 刘迎春; 宋宁宁; 石金磊

    2013-01-01

    将莱克多巴胺(RAC)与戊二酸酐反应,用混合酸酐法使反应产物与牛血清白蛋白(BSA)偶联生成免疫原,并采用淋巴细胞杂交瘤技术制备RAC单克隆抗体,建立检测猪尿、猪肉中莱克多巴胺化学发光间接竞争免疫分析法.结果显示:飞行质谱法测得RAC-戊二酸单酯分子量为416.2,RAC-戊二酸单酯与BSA偶联比为3.4∶1;RAC单克隆抗体最佳稀释倍数为l:8×104,包被抗原最佳质量浓度为0.5 μg/mL;标准曲线呈线性,回归方程为Y=-0.188 2x+0.392 2,R2=0.993 4,IC50值为0.267 ng/mL;与RAC类似化合物及其他药品无明显的交叉反应.尿样回收率为76.8% ~90.8%,批内变异系数为6.8% ~11.5%,批间变异系数为7.2% ~13.8%;猪肉样回收率为75.2%~ 87.4%,批内变异系数为7.1%~11.6%,批间变异系数为8.6%~14.1%.结果表明:用RAC-戊二酸单脂与BSA偶联物作免疫原成功地研制了针对RAC的特异性抗体,以单抗为基础建立了检测猪尿、猪肉中莱克多巴胺化学发光间接竞争免疫分析法,为进一步研制快速筛选检测试剂盒奠定了基础.%The experiment was conducted to establish an indirect competitive chemiluminescence immunoassay for determining ractopamine (RAC) residues based on monoclonal antibody (McAb). Ractopamine was coupled to bovine serum albumin (BSA) by mixture anhydrides method. The RAC-BSA conjugate was used as immunogen and McAb was prepared using lymphocytes hybridoma technology. The indirect competitive chemiluminescence immunoassay based on McAb was developed for detection and estimation of RAC residues in swine meat and urine. The molecular weight of RAC-glutaricacid mono-lipid was 416. 2 by MALDI TOF-MS/MS. The couple ratio of RAC-BSA was about 3. 4 : 1. The optimal concertration of the coating antigen (RAC-OVA) and that of McAb against RAC were 0. 5 μg/mL and 1 : 80 000, respectively. Linear detection of standard curve covered a range from 0. 0lng/mL to

  12. 鲁米诺-铁氰化钾流动注射化学发光体系测定饮用水中ɑ-茄碱%Determination of a-solanine in drinking water by flow-injection chemiluminescence system of luminol and potassium ferricyanide

    Institute of Scientific and Technical Information of China (English)

    朱定波; 马强; 燕廷; 滕久委; 唐书泽

    2012-01-01

    In order to establish a new method for rapid determination of a-solanine,a flow-injection chemiluminescence(FI-CL) system of luminol and potassium ferricyanide was developed.Results indicated that the CL reaction betweeen luminol and potassium ferricyanide was significantly reduced by a-solanine.An excellent linear range for a-solanine determination was 8.0×10-4~1.0×10-1mg/mL under the optimal condition.The limit detection of a-solanine was 6.09×10-5mg/mL and relative standard deviation for the determination was 2.1%(n=11).This method showed the advantages of simple operation,high degree of automation,easy to run on-line and real-time warning detection,and could be used as a rapid method for determination of a-solanine in drinking water.%建立一种快速测定ɑ-茄碱的新方法,利用鲁米诺-铁氰化钾化学发光体系,结合流动注射技术,对ɑ-茄碱进行测定。实验结果表明,ɑ-茄碱对鲁米诺-铁氰化钾体系发光反应有明显的抑制作用,在最佳实验条件下,该方法的线性范围为8.0×10-4~1.0×10-1mg/mL,检出限为6.09×10-5mg/mL,RSD为2.1%(n=11)。该方法具有操作简单、自动化程度高、成本低廉、容易实现在线检测以及即时预警等优点,可用于饮用水中ɑ-茄碱的快速检测。

  13. HBV serological test results comparing the use of chemiluminescence and en-zyme-linked immunosorbent assay of%乙肝病毒血清学检验采用化学发光法与酶联免疫法的效果对比

    Institute of Scientific and Technical Information of China (English)

    张怡莎

    2014-01-01

    目的:探讨化学发光免疫分析技术(ECLIA)和酶联免疫吸附试验(ELISA)在乙肝病毒血清学检验中的应用效果。方法选取2012年1月-2014年1月在我院就诊的疑似乙肝患者150例,分离血清后分别应用ELISA和ECLIA进行检测,比较两种检测方法的效果。结果ELISA和ECLIA检测出HBsAg阳性分别63例和82例,两者比较差异显著(P<0.05),同时ECLIA法检测的批内CV和批间CV的重复性均明显高于ELISA法(P<0.05)。结论与ELISA检验法比较,ECLIA法具有更高的HBsAg阳性检出率,且能进行精确的定性定量检测,值得临床推广应用。%Objective To investigate chemiluminescence immunoassay (ECLIA) and enzyme-linked immunosorbent assay (ELISA) in HBV serological test the application results. Methods Select suspected hepatitis B patients from January 2012 to January 2014 in our hospital 150 cases, respectively, after the application of separation of serum ELISA and ECLIA detection, the effect of two detection methods.Results ELISA and ECLIA detection of HBsAg positive 63 cases and 82 cases respectively,the difference was significant (P<0.05),while intra-assay and inter-assay CV CV ECLIA assay reproducibility were significantly higher than the ELISA method(P<0.05). Conclusion Compared with ELISA test method, ECLIA method has higher HBsAg positive rate,and can be accurate qualitative and quantitative detection,worthy of clinical application.

  14. Analysis of Volatile Sulfur Compounds in Waste Gases by Gas Chromatography with a Sulfur Chemiluminescence Detector%硫化学发光检测器-气相色谱法测定废气中挥发性硫化物

    Institute of Scientific and Technical Information of China (English)

    韩丛碧; 李凌波

    2012-01-01

    采用硫化学发光检测-宽口径石英毛细管柱气相色谱法,建立了废气中羰基硫、硫化氢、二硫化碳、甲硫醇、乙硫醇、甲硫醚、乙硫醚、丙硫醇、异丙硫醇、噻吩及二甲二硫等11种挥发性硫化物的分析方法.硫化学发光检测器的灵敏度、选择性及线性范围均优于传统的火焰光度检测器.进样体积为0.2 ml时,硫化物的检出限为0.03~0.1 mg/m3.实际样品5次测定的相对标准偏差小于5.1%,加标回收率为83.7%~108.8%.测定了某炼油厂酸性水罐尾气、污油罐尾气及瓦斯气中挥发性硫化物,为其恶臭控制与治理提供检测手段和基础数据.%Sulfur chemiluminescence detector-vast capillary gas chromatography has been conducted on the analysis of volatile sulfur compounds in waste gases. The recoveries of hydrogen sulfide, carbon disulfide, methyl mercaptan, ethyl mercaptan, carbon disulfide,dimethyl sulphide,diethyl sulphide, n-propyl merrcaptan, i-propyl merrcaptan, thiophene and dimethyl disulfide were from 83. 7% to 108. 8% , the relative standard deviations were less than 5. 1% . When inject volumn was 0. 2 ml, the minimum detection levels of the above-mentioned volatile sulfur compounds varied between 0.03 mg/m3and 0. 1 mg/m3 Volatile sulfur compounds in waste gases from sour water tanks and oil tanks in an refinery and Gas were analyzed.

  15. 化学发光生物传感器法测定食品中有机磷与氨基甲酸酯类农药残留%Determination of organophosphorus and carbamates pesticide residues in foods by chemiluminescence biosensor

    Institute of Scientific and Technical Information of China (English)

    王晓朋; 曾梅; 万德慧; 唐晗; 赵彬媛; 周雅倩; 刘晓宇

    2014-01-01

    Objective To create a high-sensitivity biosensor for detecting organophosphorus and carbamates pesticides in foods and establish a new method to determine these two kinds of pesticides.Methods Immobilized acetylcholinesterase (AchE) was used as recognition element of the sensor, reacting specifically with the substrate of acetylthiocholine iodide(ATCI), the micro-fluidic chip with CL analyzer was used as detecting element, and luminol-potassium ferricyanide system was used in chemiluminescence system. ResultsWhen the concentration of proximal, dichlorvos, dimethoate ranging from 0.1~10, 0.08~10, and 0.8~15μg/mL, respectively, the correlation coefficients were 0.9923, 0.9903, and 0.9904, and the detection limits were 0.047, 0.054, and 0.388μg/mL, respectively. When the concentrations of carbofuran, carbonyl, and methomyl were ranging from 0.08~15, 0.1~10, and 0.1~10μg/mL, respectively, the correlation coefficients were 0.9926, 0.9972, and 0.9944, and the detection limits were 0.049, 0.051, and 0.080μg/mL, respectively. The evaluation of biosensor performance indicates good precision, when determining all 6 kinds of pesticides under optimal conditions, RSD was below 7%; when the substrate of ATCI was injected into chemiluminescence system 6 times discontinuously, RSD was below 8%, which indicated good stability; immobilized AChE was stored in phosphate buffer(pH=8.0), detecting activity every 10 d, lasting for two months, the relative enzymatic activity decreased only 23%, indicating a good storage stability; the biosensor was used to detect samples of cabbage and apple, fortified recoveries were between 90%~99%.Conclusion This biosensor has a good performance, therefore it can be used to determine pesticides in foods.%目的:构建一种用于检测食品中有机磷与氨基甲酸酯类农药残留的高灵敏度生物传感器,建立一种用于测定食品中两类农药残留的新方法。方法以固定化乙酰胆碱酯酶(AChE

  16. Synthesis of unstable cyclic peroxides for chemiluminescence studies

    Energy Technology Data Exchange (ETDEWEB)

    Bartoloni, Fernando H.; Oliveira, Marcelo A. de; Augusto, Felipe A.; Ciscato, Luiz Francisco M.L.; Bastos, Erick L.; Baader, Wilhelm J., E-mail: wjbaader@iq.usp.br [Universidade de Sao Paulo (USP), SP (Brazil). Inst. de Quimica. Dept. de Quimica Fundamental

    2012-11-15

    Cyclic four-membered ring peroxides are important high-energy intermediates in a variety of chemi and bioluminescence transformations. Specifically, a-peroxy lactones (1,2-dioxetanones) have been considered as model systems for efficient firefly bioluminescence. However, the preparation of such highly unstable compounds is extremely difficult and, therefore, only few research groups have been able to study the properties of these substances. In this study, the synthesis, purification and characterization of three 1,2-dioxetanones are reported and a detailed procedure for the known synthesis of diphenoyl peroxide, another important model compound for the chemical generation of electronically excited states, is provided. For most of these peroxides, the complete spectroscopic characterization is reported here for the first time. (author)

  17. On chemiluminescent emission from an infiltrated chiral sculptured thin film

    CERN Document Server

    Jamaian, Siti S

    2010-01-01

    The theory describing the far-field emission from a dipole source embedded inside a chiral sculptured thin film (CSTF), based on a spectral Green function formalism, was further developed to allow for infiltration of the void regions of the CSTF by a fluid. In doing so, the extended Bruggeman homogenization formalism--which accommodates constituent particles that are small compared to wavelength but not vanishingly small--was used to estimate the relative permittivity parameters of the infiltrated CSTF. For a numerical example, we found that left circularly polarized (LCP) light was preferentially emitted through one face of the CSTF while right circularly polarized (RCP) light was preferentially emitted through the opposite face, at wavelengths within the Bragg regime. The centre wavelength for the preferential emission of LCP/RCP light was red shifted as the refractive index of the infiltrating fluid increased from unity, and this red shift was accentuated when the size of the constituent particles in our h...

  18. Research of the chemiluminescence detection apparatus for nutrients

    Science.gov (United States)

    Xu, Xiaoyi; Wang, Yu; Ni, Xuxiang; Yan, Huimin

    2016-10-01

    The multifunctional nutrition analyzer, which integrates four detection functions, can make fast, accurate, quantitative analysis for a variety of nutrients. In this article we focus on researching the luminescence detection system. Compared with other means, luminescence detection needs no excitation light, and the detection sensitivity is improved due to the reduction of the background light. The apparatus consists of an displacement platform, a microporous plate, a combination of an aspheric lens and a plano-convex lens, an optical fiber and a photon counter connected with a computer. A theoretical light intensity formula is established as a reference and a comparison of the experimental data. In the experiment we applies ATP detection reagent as the experimental reagent, whose magnitudes of concentration are from 10-6 mol/L to 10-12 mol/L. The sensitivity of the apparatus could reach a magnitude of concentration of 0.1nmol/L, and it is estimated to be further improved by at least two magnitudes in theory with the system and the reagent optimized.

  19. Applications of Chemiluminescence in the Teaching of Experimental Design

    Science.gov (United States)

    Krawczyk, Tomasz; Slupska, Roksana; Baj, Stefan

    2015-01-01

    This work describes a single-session laboratory experiment devoted to teaching the principles of factorial experimental design. Students undertook the rational optimization of a luminol oxidation reaction, using a two-level experiment that aimed to create a long-lasting bright emission. During the session students used only simple glassware and…

  20. Flow-injection Chemiluminescence Determination of Trace Calf Thymus DNA

    Institute of Scientific and Technical Information of China (English)

    Min ZHOU; Yong Jun MA; Xiao Yong JIN; Xiu Lan TENG; Zi Yu ZHANG; Hui CHEN

    2003-01-01

    A flow injection procedure for the determination of calf thymus DNA (CT DNA) overthe range 2.1x10-6~2.1×10-1 μg mL-1 is described, based on measurement of the enhancedchemiluminescence emission of rhodamine B-Ce(Ⅳ) system, activated by imidazole-HCl buffersolution. This method is highly sensitive, with the linearity range broadened to five orders ofmagnitude. It has been applied to determine CT DNA in synthetic sample with satisfactoryresults.

  1. On chemiluminescent emission from an infiltrated chiral sculptured thin film

    OpenAIRE

    Jamaian, Siti S.; Mackay, Tom G.

    2010-01-01

    The theory describing the far-field emission from a dipole source embedded inside a chiral sculptured thin film (CSTF), based on a spectral Green function formalism, was further developed to allow for infiltration of the void regions of the CSTF by a fluid. In doing so, the extended Bruggeman homogenization formalism--which accommodates constituent particles that are small compared to wavelength but not vanishingly small--was used to estimate the relative permittivity parameters of the infilt...

  2. Chemiluminescent ion-molecule reactions B + +H2

    Science.gov (United States)

    Ottinger, Ch.; Reichmuth, J.

    1981-01-01

    Emission spectra resulting from impact of low-energy (1-10 eVc.m.) B+ ions in a beam on an H2 target were measured. Numerous new bands of the BH+(A 2Π-X 2Σ+) transition were observed besides the previously known (0,0) and (1,1) bands. Also, at higher energy a new band system was discovered which is ascribed to the BH+(B' 2Σ+-X 2Σ+) transition. It was identified by analogy with BeH and MgH. The results are discussed in terms of electronic state and molecular orbital correlation diagrams.

  3. Application of Direct Chemiluminescence Immunoassay to Rapid Detection of Infectious Agents Before Endoscopy%直接化学发光免疫分析法作为传染四项快速检测方法的应用探讨

    Institute of Scientific and Technical Information of China (English)

    周俊; 袁帆; 潘涛; 安垣奇

    2016-01-01

    目的 评价直接化学发光免疫分析法(chemiluminescence immunoassay,CLIA)作为消化性内镜前传染性指标:乙型肝炎表面抗原(HBsAg)、丙型肝炎抗体(抗-HCV)、梅毒螺旋体抗体(抗-TP)和人类免疫缺陷病毒抗体(抗HIV-1/2)四个项目快速检测方法的价值.方法 对本院2015年4月13日~4月20日共561例急诊筛查传染性指标四项的住院门诊患者,同时用免疫层析法和直接化学发光免疫分析法平行检测乙型肝炎表面抗原(HBsAg)、丙型肝炎抗体(抗-HCV)、梅毒螺旋体抗体(抗-TP)和人类免疫缺陷病毒抗体(抗HIV-1/2),对上述两种方法检测出的阳性结果用ELISA方法复检,比较两种方法的检测结果.结果 561例标本免疫层析法检测阳性标本数HBsAg11例(1.96%),抗-HCV11例(1.96%),抗-TP19例(3.39%),抗-HIV-1/2 5例(0.89%),直接化学发光免疫分析法检测阳性标本数HBsAg11例(1.96%),抗-HCV4例(0.71%),抗-TP8例(1.43%),抗-HIV-1/2 3(0.53%)例.两种方法阳性标本经ELISA方法复检后阳性标本例数分别为:免疫层析法HBsAg11例(100%),抗-HCV2例(18.18%),抗-TP8例(42.11%),抗-HIV-1/2 0例(0),直接化学发光免疫分析法HBsAg11例(100%),抗-HCV2例(50%),抗-TP6例(100%),抗-HIV-1/2 0例(0).结论 在临床快速筛查HBsAg、抗-HCV、抗-TP、抗-HIV-1/2时,直接化学发光免疫分法较免疫层析法复检率低,复检后阳性率高,相对更为经济、高效、准确.

  4. Potassium permanganate-rhodamine B chemiluminescence for the determination of sulphite in food by pervaporation and flow injection%渗透汽化流动注射高锰酸钾-罗丹明B化学发光法测定食品中的亚硫酸盐

    Institute of Scientific and Technical Information of China (English)

    张红兵; 胡云霞; 连靠奇; 狄柯坪

    2013-01-01

    目的:结合渗透汽化和流动注射技术,建立一种高锰酸钾-罗丹明B化学发光测定食品中亚硫酸盐的新方法.方法:在酸性介质中,高锰酸钾氧化罗丹明B产生微弱的化学发光,SO2的存在可显著增强该体系的发光信号强度;且在一定范围内呈良好的线性关系;渗透汽化技术在酸性条件下将样品中的亚硫酸盐转化为SO2并分离出来,基于此,利用渗透汽化与化学发光联用技术进行食品中亚硫酸盐含量测定.结果:Na2 SO3浓度0.8 mg/L~4.0 mg/L范围内与发光强度呈良好的线性关系,检测限是0.2 mg/L.对2.0mg/L的Na2SO3标准溶液平行测定11次,平行测得值的RSD为4.2%,用于测定食品中亚硫酸盐含量,结果满意.结论;该方法可以用于测定食品中亚硫酸盐含量.%Objective:A chemiluminescence (CL) procedure was developed by pervaporation and flow injection of potassium permanganate-rhodamine B for determination of contaminated sulphite in food samples.Methods:The method is based on the oxidation of rhodamine B by permanganate solution in acid medium to give out weak CL signal,which is dramatically enhanced by sulphite which can be converted to sulphur dioxide and transported in pervaporation module.The CL signal is linear with sulphite concentration in a certain range.Results:Under optimum conditions,the CL emission showed linear with Na2SO3 in the concentration range of 0.8 mg/L ~ 4.0 mg/L,the detection limit was 0.2 mg/L.The relative standard deviation was 4.2% when the concentration of Na2SO3 was2.0 mg/L.Conclusion:The method is applied to the determination of sulphite in different food samples with satisfying results.

  5. A microfluidic chip-chemiluminescence sensor based on molecular imprinted recognition for determination of ractopamine%基于分子印迹聚合物的微流控化学发光传感器检测莱克多巴胺

    Institute of Scientific and Technical Information of China (English)

    万德慧; 王晓朋; 吴中波; 唐晗; 刘晓宇

    2014-01-01

    目的:制备能特异性识别莱克多巴胺(ractopamine, RCT)的分子印迹聚合物(molecularly imprinted polymers, MIPs),将该印迹聚合物与微流控化学发光法结合起来,以制备高选择性的化学发光传感器。方法利用沉淀聚合法制备莱克多巴胺印迹聚合物,采用扫描电镜、红外光谱法、吸附实验对其进行表征;以该印记聚合物为识别元件,以微流控芯片为流通反应池,并以化学发光仪作为检测器,通过流动注射分析法来检测猪肝和牛肉中的莱克多巴胺。结果在最佳条件下,测得莱克多巴胺线性范围6∼960 ng/mL,线性回归方程为∆I=4.8777C-9.7851, r=0.9907,检出限为0.83 ng/mL,对10 ng/mL莱克多巴胺平行测定11次,其RSD(相对标准偏差)为7.1%。应用该方法成功分析了猪肝和牛肉中RCT含量,回收率可达90.3%~99.8%。结论本方法快速、准确、样品前处理简单,能很好地用于食品中莱克多巴胺残留的检测。%Objective A ractopamine (RCT) imprinted polymer was prepared as a recognition element, which had the ability of specific recognition. Based on the recognition element, a novel microfluidic chip-chemiluminescence(CL) sensor was designed and assembled to monitor the RCT content in food. Methods The RCT imprinted polymer was synthesized by precipitation polymerization. SEM, FT-IR spectra and absorption experiment were applied as characterization methods. With MIPs working as the recognition element, microfluidic chip as flow reaction cell, a novel flow analysis CL sensor for the determination of RCT was developed and optimized. Results Under the optimal conditions, the relative CL intensity showed a linear relationship in the range of 6∼960 ng/mL RCT with detection limit of 0.83 ng/mL. The linear regression equation was ΔI=4.8777C-9.7851, r=0.9907. The relative standard deviation was 7.1% for 10 ng/ mL RCT (n=11). The method was successfully used to analyze the RCT content in pig liver and beef

  6. Evaluation of detection performance of DXI800 chemiluminescence analyzer according to EP10-A2 document of CLSI%应用 CLSI EP10-A2文件对 DXI800化学发光仪性能评价的结果分析

    Institute of Scientific and Technical Information of China (English)

    杨方华; 杨宝瑞; 马东礼; 邓芳梅; 孙莉芳

    2014-01-01

    目的:应用CLSI EP10-A2文件初步评价DXI800化学发光仪的分析性能。方法严格按照 EP10-A2文件要求,连续5 d按特定顺序测定高、中、低浓度质控血清中的人生长激素(hG H )浓度,采用 Excel软件计算hG H测定的偏差、总不精密度及每天的多元回归分析参数(即截距、斜率、非线性、携带污染率及漂移度)。结果当质控血清hGH浓度为2.15、5.47、10.80 mmol/L时,hGH测定值与靶值间线性良好(Y =0.992X+0.083,r2=0.978);偏差分别为0.066、0.265、0.055 mmol/L ;总不精密度分别为6.57%、8.53%、4.56%;采用单样本 t检验,每个多元回归分析参数5 d的值与其预测值相比,差异均无统计学意义( P>0.05)。结论 DXI800化学发光仪测定hG H ,其偏差及总不精密度均在允许范围内,线性关系良好,携带污染率较低,稳定性良好,能满足临床及科研需求。%Objective To evaluate the detection performance of DXI800 chemiluminescence analyzer (DXI800 analyzer) according to EP10-A2 document of Clinic and Laboraroty Standard Institute (CLSI) .Methods According to EP10-A2 document ,human growth hormone (hGH) in quality control serum were continuously detected for five days with a certain order ,then the deviation ,total imprecision ,intercept ,slope rate ,non-linearity ,cross-contami-nation and drift were analyzed by using Microsoft Excellsoftware .Results The linearity between measured value and target value was relatively well (Y=0 .992X+0 .083 ,r2 =0 .978) when the concentrations of hGH were 2 .15 ,5 .47 and 10 .80 mmol/L ,with bias of 0 .066 ,0 .265 and 0 .055 mmol/L ,and total imprecision of 6 .57% ,8 .53% and 4 .56% ,respectively .Simple sample t test indicated that the difference of intercept ,slope rate ,non-linearity ,cross-contamination and drift was no statistically significant (P>0 .05) .Conclusion The deviation ,total imprecision ,in

  7. Clinical application of chemiluminescence enzyme immunoassay to quantitatively detect human thyroid peroxidase antibodies%定量检测人甲状腺过氧化物酶抗体化学发光酶免疫分析法的临床应用

    Institute of Scientific and Technical Information of China (English)

    柴锦燕; 方佩华; 谭建; 李宁

    2016-01-01

    Objective To apply chemiluminescence enzyme immunoassay (CLEIA) kits of thyroid peroxidase antibodies (hTPOAb) on normal and all kinds of patients with thyroid diseases, and explore the clinical value of the kits.Methods The hTPOAb kits were used in 333 cases of serum samples, including 133 cases of healthy people, 94 cases of Hashimoto's thyroiditis(HT) ,68 cases of Graves' disease(GD), 19 cases of nodular goiter, 10 cases of subacute thyroiditis and 9 cases of simple goiter.Results Concentration above 3.22 IU/ml was considered to be positive of the hTPOAb kits.The median concentration of hTPOAb was 5.48 IU/ml in HT (positive ratio was 91.50%), 1.88 IU/ml in GD (positive ratio was 29.40%), 1.83 IU/ml in nodular goiter(positive ratio was 10.50%) ,2.54 IU/ml in subacute thyroiditis(positive ratio was 20.00%) ,and 2.21 IU/ml in simple goiter(positive ratio was 0).The positive ratio of hTPOAb in patients with HT was higher than that of the other patients (x2 =67.22,60.13,35.49,49.89, all P < 0.01).Conclusion The CLEIA kits of hTPOAb has a high positive rate for HT, and is an effective method in diagnosing HT.%目的 应用人甲状腺过氧化物酶抗体(hTPOAb)的化学发光酶免疫分析(CLEIA)试剂盒,检测正常人及各类甲状腺疾病患者,探讨该试剂盒在临床应用中的价值.方法 用该试剂盒共测定333例样品血清中的hTPOAb,包括正常人133名,各类甲状腺疾病患者200例,其中桥本甲状腺炎(HT) 94例,Graves病68例,结节性甲状腺肿19例,亚急性甲状腺炎10例,单纯性甲状腺肿9例.结果 确立本试剂盒hTPOAb阳性切限值为3.22 IU/ml,hTPOAb浓度以中位数表示,分别为HT5.48 IU/ml,阳性率为91.50%;Graves病1.88 IU/ml,阳性率为29.40%;结节性甲状腺肿1.83 IU/ml,阳性率为10.50%,亚急性甲状腺炎2.54 IU/ml,阳性率为20.00%;单纯性甲状腺肿2.21 IU/ml,阳性率为0.HT患者血清hTPOAb阳性率高于其他患者(x2=67.22,60.13,35.49,49.89,P均<0.01).结论

  8. Detection of drug residues in fish and fishery products:comparison between chemiluminescence enzyme-linked immunoassay and enzyme linked immunosorbent assay%化学发光免疫分析与酶联免疫分析法检测水产品药物残留的比较研究

    Institute of Scientific and Technical Information of China (English)

    萨仁托雅; 张峰; 郑有虎; 卢亚楠

    2014-01-01

    Detection of furazolidone metabolite ( AOZ ) and chloramphenicol ( CAP ) residues in fish and fishery products was comparatively studied by chemiluminescence enzyme-linked immunoassay ( CLEIA ) and traditional colorimetric enzyme-linked immunosorbent assay ( ELISA) . The results showed that CLEIA had better linear range and detection limit than ELISA, and a close correlation of recovery rate was shown in the two methods. In CLISA, the detection limit was about 0. 01 μg/kg for AOZ, and about 0. 016 μg/kg for CAP and the linear range was from 0. 01 to 2. 56μg/kg for AOZ, and from 0. 025μg/kg to 6. 400μg/kg for CAP. In ELISA, however, the detection limit was 0 . 1 μg/kg for AOZ and about 0 . 05 μg/kg for CAP and the linear range was from 0 . 1 to 1 . 62 μg/kg for AOZ and from 0. 05 to 4. 05 μg/kg for CAP. The satisfactory precisions of the assay were found in CLEIA, with variation coefficient of 5. 5%-11. 3% in the intra-assay and 12. 3%-20. 9% in the inter-assay for CAP, and with variation coefficient of 6. 6-11. 1% in the intra-assay and 15. 6%-18. 3% in the inter-assay for AOZ, within the record reference evaluation standard of ELISA residues of veterinary drugs ( kit) .%对检测水产品中呋喃唑酮代谢物( AOZ)和氯霉素( CAP)药物残留的两种方法---化学发光免疫分析( CLEIA)和酶联免疫分析( ELISA)法进行了比较研究。结果表明:两种药物残留检测中, CLEIA分析方法的线性范围和检出限均优于ELISA法,且两种免疫分析法的添加回收率均具有很好的相关性;用CLEIA法检测AOZ的检出限为0.01μg/kg,线性范围为0.01~2.56μg/kg,检测CAP的检出限为0.016μg/kg,线性范围为0.025~6.400μg/kg;用ELISA法检测AOZ的检出限为0.1μg/kg,线性范围为0.10~1.62μg/kg,检测CAP的检出限为0.05μg/kg,线性范围为0.05~4.05μg/kg;用CLEIA法检测CAP的批内变异系数( RSD)为5.5%~11.3%,批间RSD为12.3%~20.9%,检测AOZ的批内RSD为6.6%~11.1%,批间RSD为15.6%~18.3%

  9. Low Intensity, Long Duration Chemiluminescent Systems. Part 2. Chemiluminescence from BIS(2,4-Dichloro-6-Carboalkoxyphenyl)Oxalates,

    Science.gov (United States)

    1979-01-01

    T. and Schaum , C. (1894) . Chem. Ber., 27, 537. 2. Konig, W. (1904). .7. prakt. Chea., 2, 70, 19. 23. Graebe, C. (1868). Annalen, 146, 1. l - 24...Chief Superintendent Superintendent, Organic Chemistry Division Dr. D.E. Paul Mr. C.D. Dowd Library Librarian, N.S.W. Branch (Through Officer—in-Charge

  10. 使用化学发光法检测26707例血清抗梅毒螺旋体特异性抗体以及结果假阳性率分析%Analyze of the False Positive Rate of Serum Anti Treponema Pallidum Specific Antibody of 26 707 Cases Detected by Chemiluminescence Method

    Institute of Scientific and Technical Information of China (English)

    张保平; 刘珊; 韩艳秋

    2015-01-01

    Objective To detect serum anti-Treponema pallidum specific antibody of 26 707 cases by Abbott I2000SR auto-matic chemiluminescent microparticle immunoassay analyzer,and treponema pallidum particle agglutination assay (TPPA) was regarded as a standard reference method which was used to detect anti-Treponema pallidum specific antibody.To analyze the false positive rate of Abbott I2000SR according to the TPPA.Methods Collected 26 707 serums from inpatients and outpatients of the hospital during September 1,2013 to March 5,2014.The subjects were asked to fasting conditions taking venous blood 3 ml,3 000 r/min centrifugal 10 min utes after the separation of serum,detected the Anti-TP by CMIA (Ab-bott I2000SR)and the TPPA testing,analyzed test results by statistical methods.Results There were 52 cases detected by I2000SR whose S/CO values of 26 707 cases of serum Treponema pallidum specific antibodies were 1 to 2,of which 9 cases were verified positive by TPPA,and the positive rate was 17.31%.There were 26 cases detected by I2000SR whose S/CO values of Treponema pallidum specific antibodies were 2 to 3,of which 9 cases were verified positive by TPPA,and the posi-tive rate was 34.62%.There were 26 cases detected by I2000SR whose S/CO values of Treponema pallidum specific anti-bodies were 3 to 5,of which 9 cases were verified positive by TPPA,and the positive rate was 34.62%.There were 25 cases detected by I2000SR whose S/CO values of Treponema pallidum specific antibodies were 5 to 7,of which 11 cases were veri-fied positive by TPPA,and the positive rate was 44%.There were 25 cases detected by I2000SR whose S/CO values of Treponema pallidum specific antibodies were 7 to 10,of which 17 cases were verified positive by TPPA,and the positive rate was 68%.There were 28 cases detected by I2000SR whose S/CO values of Treponema pallidum specific antibodies were 10to 13,of which 24 cases were verified positive by TPPA,and the positive rate was 85.71%.There were 23 cases detected

  11. 不同抗HCV化学发光标记免疫分析法试剂S/CO值与重组免疫印迹确证实验的相关性研究%The correlation between S/CO ratios of different chemiluminescence assays and recombinant immunoblot in anti-HCV antibody detection

    Institute of Scientific and Technical Information of China (English)

    王立水; 徐明杰; 江长林; 王佳颖; 杨燕; 欧兰香

    2016-01-01

    目的 分析不同厂家丙型肝炎病毒抗体(抗HCV)化学发光法(CLIA)筛查试剂S/CO值与重组免疫印迹(RIBA)确证实验阳性结果的相关性.方法 采用病例对照研究.应用3种国产试剂(山东莱博、北京源德、北京科美)和1种进口试剂(美国雅培)对2014年3月至2015年3月山东大学齐鲁医院检验科收集的2 616例血清样本进行抗HCV筛查,反应性标本再通过RIBA进一步确证.对阳性预测值与抗HCV S/CO值的相关性分析比较.不同试剂间差异比较采用x2检验方法.结果 山东莱博1≤S/CO< 26.8时,阳性预测值为33.3%,S/CO ≥26.8时,阳性预测值为97.8%;北京源德1≤S/CO< 16.6时,阳性预测值为20%,S/CO≥16.6时,阳性预测值为96.7%;北京科美1≤S/CO< 16.7时,阳性预测值为9.8%,S/CO≥16.7时,阳性预测值为97.0%;美国雅培1≤S/CO<5时,阳性预测值为12.8%,S/CO≥5时,阳性预测值为96.9%.结论 抗HCV CLIA筛查试剂盒检测S/CO值与RIBA确证实验阳性结果有一定相关性,国产试剂可参考进口试剂确定S/CO值与阳性预测值的关系.不同试剂相同阳性预测值对应不同S/CO值,提示厂家可根据情况设定产品特定S/CO值,为临床实验结果判定提供参考.%Objective To investigate relationships between signal/cutoff (S/CO) ratios of antiHCV recombinant immunoblot assay (RIBA) and their positivity with different chemiluminescence immunoassay(CLIA) reagents.Methods A case-control study was performed.From March 2014 to March 2015,anti-HCV antibody was detected in 2 616 serum of outpatients and inpatients coming from Department of Clinical Laboratory,Qilu Hospital of Shandong University by three kinds of homemade CLIA reagents and one imported CLIA reagents.The positive samples were further tested by RIBA.The correlation between the positivity and the S/CO ratios was analyzed.The difference between different reagents were compared by x2 method.Results The predicted positivities of

  12. Study on prenatal screening for birth deficiency in the pregnant metaphase by the serum AFP, β-HCG and uE3 with chemiluminescent immunoassay%联合检测孕中期血清甲胎蛋白、β-绒毛膜促性腺激素、游离雌三醇筛查出生缺陷的应用研究

    Institute of Scientific and Technical Information of China (English)

    邱振华; 高艳; 吴玉华; 戚子惠; 黄金波; 舒云华; 陈宇锋

    2009-01-01

    Objective To evaluate the effect of the serum markers of AFP, β-HCG and uE3 on screening of congenital fetal abnormality in the pregnant women at second trimester. Methods The serum markers were measured in 2 149 pregnant women at second trimester of gestation with chemiluminescent immunoassay, and were combined with correlation factors such as the age, gestational weeks and the weight of pregnant women, to estimate the probability of fetus suffering from Down's syndrome, open spina bifida and Trisomy 18 syndrome. Cell chromosomes and ultrasonography were also done in high risk patients. Analysis was carried out based on the results of follow-up survey. Results One hundred and thirty-six (6.33%) cases wind high risk of birth deficiency were found in preliminary screening from 2 149 pregnant women, in which the positive rates of Down's syndrome, open spina bifida and Trisomy 18 syndrome were 4.14%, 1.77% and 0.42%,respectively. The positive rates were 66.67% and 100% and the false positive rates were 3.75% and 1.85% in Down's syndrome and open spina bifida. Conclusion The combined screening of maternal serum markers of AFP, β-HCG and uE3 is reliable and effective, and is the important measure to reduce birth deficiencify combined with prenatal diagnosis.%目的:评价联合检测孕妇孕中期(14~19+6周)血清标志物甲胎蛋白、β-绒毛膜促性腺激素、游离雌三醇在产前筛查先天出生缺陷中的应用价值.方法:应用化学发光免疫分析技术检测2 149例孕妇孕中期血清标志物,并结合孕妇年龄、孕周、体质量等因素,应用仪器配套的产前筛查风险分析软件计算唐氏综合征、18-三体综合征和开放性脊柱裂的风险率,对高风险孕妇采用B超和/或细胞染色体检查进行确认,根据随访及诊断结果进行评估.结果:2 149例孕妇筛查出出生缺陷高风险136例,筛查阳性率为6.33%,其中唐氏综合征、开放性脊柱裂和18-三体综合征

  13. Exercício físico e estresse oxidativo: efeitos do exercício físico intenso sobre a quimioluminescência urinária e malondialdeído plasmático Ejercício físico y estres oxidativo: efectos del ejercício físico intenso sobre la quimioluminiscencia urinaria y el malondialdheído plasmático Physical exercise and oxidative stress: effect of intense physical exercise on the urinary chemiluminescence and plasmatic malondialdehyde

    Directory of Open Access Journals (Sweden)

    Tácito Pessoa de Souza Jr.

    2005-02-01

    exercise causes oxidative stress in animals and humans, being possibly related, for instance, to fatigue and tissue lesions. However, the effects of high intensity exercise or training performed by athletes on the occurrence of oxidative stress are not fully clear, possibly due to methodological limitations. The objective of this study was to identify the occurrence of oxidative lesions in lipids due to physical training in athletes, through the quantification of the urinary chemiluminescence and plasmatic malondialdehyde (MDA. Post-exercise samples were collected after four training protocols: a treadmill running (25-30 min; b 20 km running performed by marathon runners; c interval training accomplished by 400 m runners; d soccer game with 50 min duration; and e strength training with and without creatine supplementation. In the last four items, only the urinary chemiluminescence was evaluated. The conditions that presented elevation in urinary chemiluminescence after exercise completion were: a 20 km running; b soccer game; and c strength training without creatine supplementation. The treadmill running increased plasmatic MDA concentration during and after its performance, and the plasmatic antioxidant capacity had an inverse behavior compared to the increase in MDA. The exercise used in this work promoted oxidative stress in a different way and this may be related to the duration and the intensity performed by athletes, and not only to intensity. In this work it was also observed that creatine ingestion associated with strength training might work as antioxidant.

  14. Evaluation of the antioxidant effects of four main theaflavin derivatives through chemiluminescence and DNA damage analyses

    Institute of Scientific and Technical Information of China (English)

    Yuan-yuan WU; Wei LI; Yi XU; En-hui JIN; You-ying TU

    2011-01-01

    Theaflavins (TFs) are the dimers of a couple of epimerized catechins,which are specially formed during black tea fermentation.To explore the differences among four main TF derivatives (theaflavin (TF1),theaflavin3-gallate (TF2A),theaflavin-3′-gallate (TF2B),and theaflavin-3,3′-digallate (TF3)) in scavenging reactive oxygen species (ROS) in vitro,their properties of inhibiting superoxide,singlet oxygen,hydrogen peroxide,and the hydroxyl radical,and their effects on hydroxyl radical-induced DNA oxidative damage were systematically analyzed in the present study.The results show that,compared with (-)-epigallocatechin gallate (EGCG),TF derivatives were good antioxidants for scavenging ROS and preventing the hydroxyl radical-induced DNA damage in vitro.TF3 was the most positive in scavenging hydrogen peroxide and hydroxyl radical,and TF1 suppressed superoxide.Positive antioxidant capacities of TF2B on singlet oxygen,hydrogen peroxide,hydroxyl radical,and the hydroxyl radical-induced DNA damage in vitro were found.The differences between the antioxidant capacities of four main TF derivatives in relation to their chemical structures were also discussed.We suggest that these activity differences among TF derivatives would be beneficial to scavenge different ROS with therapeutic potential.

  15. Electrogenerated chemiluminescence induced by sequential hot electron and hole injection into aqueous electrolyte solution

    Energy Technology Data Exchange (ETDEWEB)

    Salminen, Kalle; Kuosmanen, Päivi; Pusa, Matti [Aalto University, Department of Chemistry, Laboratory of Analytical Chemistry, P.O. Box 16100, FI-00076 Aalto (Finland); Kulmala, Oskari [University of Helsinki, Department of Physics, P.O. Box 64, FI-00014 (Finland); Håkansson, Markus [Aalto University, Department of Chemistry, Laboratory of Analytical Chemistry, P.O. Box 16100, FI-00076 Aalto (Finland); Kulmala, Sakari, E-mail: sakari.kulmala@aalto.fi [Aalto University, Department of Chemistry, Laboratory of Analytical Chemistry, P.O. Box 16100, FI-00076 Aalto (Finland)

    2016-03-17

    Hole injection into aqueous electrolyte solution is proposed to occur when oxide-coated aluminum electrode is anodically pulse-polarized by a voltage pulse train containing sufficiently high-voltage anodic pulses. The effects of anodic pulses are studied by using an aromatic Tb(III) chelate as a probe known to produce intensive hot electron-induced electrochemiluminescence (HECL) with plain cathodic pulses and preoxidized electrodes. The presently studied system allows injection of hot electrons and holes successively into aqueous electrolyte solutions and can be utilized in detecting electrochemiluminescent labels in fully aqueous solutions, and actually, the system is suggested to be quite close to a pulse radiolysis system providing hydrated electrons and hydroxyl radicals as the primary radicals in aqueous solution without the problems and hazards of ionizing radiation. The analytical power of the present excitation waveforms are that they allow detection of electrochemiluminescent labels at very low detection limits in bioaffinity assays such as in immunoassays or DNA probe assays. The two important properties of the present waveforms are: (i) they provide in situ oxidation of the electrode surface resulting in the desired oxide film thickness and (ii) they can provide one-electron oxidants for the system by hole injection either via F- and F{sup +}-center band of the oxide or by direct hole injection to valence band of water at highly anodic pulse amplitudes. - Highlights: • Hot electrons injected into aqueous electrolyte solution. • Generation of hydrated electrons. • Hole injection into aqueous electrolyte solution. • Generation of hydroxyl radicals.

  16. Experimental Study on the Atomization and Chemiluminescence Characteristics of Ethanol Flame

    Directory of Open Access Journals (Sweden)

    Qing Zhang

    2017-01-01

    Full Text Available The breakup regime in ethanol diffusion flame under different conditions was studied by the high speed camera system combined with the UV camera system. Spray angle and Weber number (We were used to represent the change of breakup regime. With the increases of spray angle and We, the breakup mode changes from the Rayleigh-type breakup regime to the superpulsating regime. The reaction area and intensity of ethanol flames under different breakup regimes could be discussed by the OH⁎ distribution. From Rayleigh-type breakup regime to superpulsating breakup regime, the OH⁎ distribution increased and the oxidation-reduction reaction area expanded. At the condition of superpulsating breakup mode, the intensity of OH⁎ was significantly higher than that of other modes. The flame luminous length can be obtained by the OH⁎ emission, and OH⁎ distribution reflects the structure of flame. When the breakup regime changes from the fiber-type breakup regime to the superpulsating regime, the flame luminous length increases suddenly.

  17. Sequential Injection Determination of D-Glucose by Chemiluminescence Using an Open Tubular Immobilised Enzyme Reactor

    DEFF Research Database (Denmark)

    Liu, Xuezhu; Hansen, Elo Harald

    1996-01-01

    . The operating parameters were optimised by fractional factorial screening and response surface modelling. The linear range of D-glucose determination was 30-600 mu M, With a detection limit of 15 mu M using a photodiode detector. The sampling frequency was 54 h(-1). Lower LOD (0.5 mu M D-glucose) could...

  18. Flow injection chemiluminescence determination of loxoprofen and naproxen with the acidic permanganate-sulfite system

    Institute of Scientific and Technical Information of China (English)

    Li-Juan Wang; Yu-Hai Tang; Yang-Hao Liu

    2011-01-01

    A novel flow injection chemiluminescence(CL)method for the determination of loxoprofen and naproxen was proposed based on the CL system of KMnO4 and Na2SO3 in acid media.The CL intensity of KMnO4-Na2SO3 was greatly enhanced in the presence of loxoprofen a

  19. Flux measurements of reactive nitrogen compounds using a chemiluminescence analyser with different converter types

    Science.gov (United States)

    Ammann, Christof; Wolff, Veronika

    2015-04-01

    The availability of reactive nitrogen (Nr) is a key limiting factors for the productivity and the competition success of individual species. On the other hand, certain nitrogenous compounds can also be emitted from natural or managed ecosystems. Thus the quantification of the Nr exchange can be essential for the interpretation of ecosystem behavior. For the observation of Nr dry deposition and emission the eddy covariance (EC) method is preferable since it does not modify the environmental conditions of the ecosystem, is less prone to wall effects than chamber methods, and is less affected by gas phase chemical reactions than gradient methods. Since the various Nr compounds can undergo fast chemical reactions and have differing chemical and physical characteristics, a variety of detection techniques is usually necessary that often cannot meet the fast response requirements of the EC technique. Here we show applications of a fast response 2-channel NO analyzer suitable for EC measurements. In combination with different inlet converters (photolytic converter, gold catalyst converter, and high-temperature steel converter), the system could alternatively be used for flux measurements of NO2, NOy, and total Nr. The quantification of By combining the 2-channel analyzer with the NOy and total Nr converter simultaneously, the NH3 flux could be determined from the difference between the two channels. Concentration and flux measurements of the system were verified by inter-comparison with other methods. Potential problems include the damping of high-frequency fluctuations in the inlet system. It is therefore important to place the converter close to the sampling inlet and to quantify and correct the damping effects. Moreover, like most other flux measurement techniques, the system is susceptible to non-stationary trace gas concentrations that often occur near pollution sources.

  20. A comparison of the presumptive luminol test for blood with four non-chemiluminescent forensic techniques.

    Science.gov (United States)

    Webb, Joanne L; Creamer, Jonathan I; Quickenden, Terence I

    2006-01-01

    Presumptive blood detection tests are used by forensic investigators to detect trace amounts of blood or to investigate suspicious stains. Through the years, a number of articles have been published on the popular techniques of the day. However, there is no single paper that critiques and compares the five most common presumptive blood detection tests currently in use: luminol, phenolphthalein (Kastle-Meyer), leucomalachite green, Hemastix and the forensic light source. The present authors aimed to compare the above techniques with regard to their sensitivity, ease of use and safety. The luminol test was determined to be the most sensitive of the techniques, while Hemastix is a suitable alternative when the luminol test is not appropriate.