WorldWideScience

Sample records for chemiluminescence

  1. Low-temperature chemiluminescence

    International Nuclear Information System (INIS)

    The literary data on solid phase chemiluminescence reactions in cryogenic matrices of nitrogen and nobel gases in the 4.2-45 deg K temperature range as well as in hydrocarbon matrices at 77 deg K and higher are generalized. The mechanisms, kinetics and chemiluminescence of low-temperature chemical, radiation chemical and electrochemical reactions with the participation of uranium and xenon compounds in frozen sulfuric acid solutions, reactiong with the participation of NaBH4 and ruthenium complexes in frozen aqueous and organic solutions in 77-273 deg K are considered. the possibility of using low-temperature chemiluminescence to study the kinetics of heating and cooling is discussed

  2. Chemiluminescence immunoassay for chloramphenicol

    International Nuclear Information System (INIS)

    A simple, solid-phase chemiluminescence immunoassay (CLIA) for the measurement of Chloramphenicol(CAP) in foodstuffs is described. A rabbit anti-CAP IgG is passively adsorbed onto the walls of polypropylene plates. The labeled conjugant is horseradish peroxidase(HRP) conjugate of CAP. Luminol solution is used as the substrate of HRP. The light yield is inversely proportional to the concentration of CAP. The method has a similar sensitivity (0.05 ng/mL), specificity, precision, and accuracy to a conventional enzyme immunoassay (EIA). The intra-assay and inter-assay CVs of ten samples were <8 and <20%, respectively, and the analytical recovery of the method was 87% 100%. The experimental correlation coefficient of dilution was found to be 0.999 using milk supernatant as buffer. The assay range for the method was 0.1-10 ng/mL, and it displayed good linearity. (authors)

  3. Chemiluminescence and bioluminescence microbe detection

    Science.gov (United States)

    Taylor, R. E.; Chappelle, E.; Picciolo, G. L.; Jeffers, E. L.; Thomas, R. R.

    1978-01-01

    Automated biosensors for online use with NASA Water Monitoring System employs bioluminescence and chemiluminescence techniques to rapidly measure microbe contamination of water samples. System eliminates standard laboratory procedures requiring time duration of 24 hours or longer.

  4. Chemiluminescent prediction of service life

    Science.gov (United States)

    Hassell, J. A.; Mendenhall, G. D.; Nathan, R. A.

    1976-01-01

    Technique can be used to predict polymer degradation under actual expected-use conditions, without imposing artificial conditions. Smooth or linear correlations are obtained between chemiluminescence and physical properties of purified polymer gums.

  5. A New Chemiluminescent Triazine Reagent

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A new chemiluminescent reagent 7-(4,6-dichloro-1,3,5-triazinylamino)-4-methyl-coumarin (DTMC) was synthesized by linking 7-amino-4-methylcoumarin to cyanuric chloride at 0-5 ° C, and with it a novel chemiluminescence method was developed for the determination of hydrogen peroxide. The selectivity of this method is high, and most of the transition metal ions have no effect on the determination of H2O2.

  6. Chemiluminescence methods (present and future

    Directory of Open Access Journals (Sweden)

    Navas, M. J.

    2002-03-01

    Full Text Available This article provides a general review of chemiluminescent methods in some of their recent applications in drug analysis, sea water analysis or antioxidant activity of natural and synthetic products (including olive oil. Practical considerations are not included in the review since the main interest is to state, through the aforementioned applications, that chemiluminescence has been, is, and will be a versatile tool for Analytical Chemistry in future years.Este artículo da una visión general de los métodos de quimiluminiscencia en algunas de las aplicaciones más recientes en análisis de drogas, análisis del agua marina o la actividad antioxidante de productos naturales y de síntesis (incluyendo el aceite de oliva. Las consideraciones prácticas no están incluidas ya que el principal interés es establecer, a través de las aplicaciones mencionadas, que la quimiluminiscencia ha sido, es y será en los próximos años una herramienta versátil de la Química Analítica.

  7. Analytical Applications of Bioluminescence and Chemiluminescence

    Science.gov (United States)

    Chappelle, E. W. (Editor); Picciolo, G. L. (Editor)

    1975-01-01

    Bioluminescence and chemiluminescence studies were used to measure the amount of adenosine triphosphate and therefore the amount of energy available. Firefly luciferase - luciferin enzyme system was emphasized. Photometer designs are also considered.

  8. Chemiluminescence of indole and its derivatives

    Science.gov (United States)

    Vasil'ev, Rostislav F.; Trofimov, A. V.; Tsaplev, Yuri B.

    2010-02-01

    The results of studies on chemiluminescence of indole and its derivatives are critically analyzed. It is shown that chemical transformations of indoles lead, depending on the structure and experimental conditions, to various electronically excited products and emission of light. Many reactions considered are used as a basis for highly sensitive methods for detection of indoles in biology, medicine, ecology and forensics.

  9. Real-time chemiluminescence imaging using nano-lantern probes.

    Science.gov (United States)

    Arai, Yoshiyuki; Nagai, Takeharu

    2014-01-01

    Chemiluminescence imaging can be performed without excitation light sources at various spatial levels ranging from a single cell to the whole body. Thus far, chemiluminescence imaging has been primarily performed with long exposure times because of weak signals, resulting in low temporal resolution. Recently, the brightest-known chemiluminescent proteins--Nano-lantern and Nano-lantern-based functional indicators--have been developed. Nano-lantern probes break the limitation of temporal resolution and enable chemiluminescence imaging of living samples such as cells, plants, and small animals at video rates. This unit describes one protocol for observation of a freely moving unshaved mouse transplanted with Nano-lantern-expressing tumor cells, and another for compatible use of optogenetic tools and a Nano-lantern calcium indicator. Both protocols utilize the synchronization of illumination and camera acquisition sessions, thereby enabling real-time chemiluminescence imaging.

  10. Chemiluminescence methods for the determination of ofloxacin

    International Nuclear Information System (INIS)

    Ofloxacin is a synthetic fluoroquinolone antibiotic that has been used in the treatment of respiratory tract, urinary tract and tissue-based infections. Methodology for the determination of ofloxacin based on chemiluminescence detection can be divided into: direct oxidation with tris(2,2'-bipyridyl)ruthenium(III) or permanganate; and enhancement of the emission from either the oxidation of sulfite or the reaction between sodium nitrite and hydrogen peroxide. In this paper, we compare the analytical methodology and evaluate the light-producing pathways that have been proposed for these reactions

  11. Chemiluminescence methods for the determination of ofloxacin

    Energy Technology Data Exchange (ETDEWEB)

    Francis, Paul S. [School of Biological and Chemical Sciences, Deakin University, Geelong, Vic. 3217 (Australia)]. E-mail: psf@deakin.edu.au; Adcock, Jacqui L. [School of Biological and Chemical Sciences, Deakin University, Geelong, Vic. 3217 (Australia)

    2005-06-13

    Ofloxacin is a synthetic fluoroquinolone antibiotic that has been used in the treatment of respiratory tract, urinary tract and tissue-based infections. Methodology for the determination of ofloxacin based on chemiluminescence detection can be divided into: direct oxidation with tris(2,2'-bipyridyl)ruthenium(III) or permanganate; and enhancement of the emission from either the oxidation of sulfite or the reaction between sodium nitrite and hydrogen peroxide. In this paper, we compare the analytical methodology and evaluate the light-producing pathways that have been proposed for these reactions.

  12. Detection of hydrogen peroxide with chemiluminescent micelles

    Directory of Open Access Journals (Sweden)

    Dongwon Lee

    2008-08-01

    Full Text Available Dongwon Lee1, Venkata R Erigala1,3, Madhuri Dasari1, Junhua Yu2, Robert M Dickson2, Niren Murthy11The Wallace H. Coulter Department of Biomedical Engineering; 2Department of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA; 3The Scripps Research Institute, La Jolla, CA, USAAbstract: The overproduction of hydrogen peroxide is implicated in the progress of numerous life-threatening diseases and there is a great need for the development of contrast agents that can detect hydrogen peroxide in vivo. In this communication, we present a new contrast agent for hydrogen peroxide, termed peroxalate micelles, which detect hydrogen peroxide through chemiluminescence, and have the physical/chemical properties needed for in vivo imaging applications. The peroxalate micelles are composed of amphiphilic peroxalate based copolymers and the fluorescent dye rubrene, they have a ‘stealth’ polyethylene glycol (PEG corona to evade macrophage phagocytosis, and a diameter of 33 nm to enhance extravasation into permeable tissues. The peroxalate micelles can detect nanomolar concentrations of hydrogen peroxide (>50 nM and thus have the sensitivity needed to detect physiological concentrations of hydrogen peroxide. We anticipate numerous applications of the peroxalate micelles for in vivo imaging of hydrogen peroxide, given their high sensitivity, small size, and biocompatible PEG corona.Keywords: hydrogen peroixde, chemiluminescence, micelles, amphiphilic copolymer

  13. Determination of ribavirin by flow injection chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    Juqing Jin; Yuhai Tang; Langchong He; Hong Yao; Yuanyuan Sun

    2006-01-01

    Objective: To establish a rapid and precise continuous flow-injection chemiluminescence (CL) method to determine ribavirin. Methods: The ribavirin could restrain strongly CL reaction of luminol in sodium hydroxide (NaOH) solution with potassium chlorate(KClO3). The different experimental parameters affecting the CL intensity were studied carefully. Results:Under optimum conditions(The concentrations of luminol,KClO3, and NaOH were at 0.1 mmol/L,0.5 μmol/L,and 0.2 mol/L, respectively), the linear range of the working curves was 0.01-7.00 μg/ml with a detection limit of 0.004 μg/ml. Conclusion: The method is simple, rapid and sensitive, and successfully applied to the determination of ribavirin in pharmaceutical preparations and biological fluids.

  14. Evaluation of glycophenotype in prostatic neoplasm by chemiluminescent assay

    OpenAIRE

    da Silva, Lúcia Patrícia Bezerra Gomes; Almeida, Sinara Mônica Vitalino; Lima, Luiza Rayanna Amorim; Cavalcanti, Carmelita de Lima Bezerra; Lira, Mariana Montenegro de Melo; da Silva, Maria da Paz Carvalho; Beltrão, Eduardo Isidoro Carneiro; Júnior, Luiz Bezerra de Carvalho

    2014-01-01

    This work aimed to evaluate the glycophenotype in normal prostate, bening prostatic hyperplasia (BPH) and prostatic adenocarcinoma (PCa) tissues by a chemiluminescent method. Concanavalin A (Con A), Ulex europaeus agglutinin (UEA-I) and Peanut agglutinin (PNA) lectins were conjugated to acridinium ester (lectins-AE). These conjugates remained capable to recognize their specific carbohydrates. Tissue samples were incubated with lectins-AE. The chemiluminescence of the tissue-lectin-AE complex ...

  15. A novel glucose chemiluminescence biosensor based on a rhodanine derivative chemiluminescence system and multilayer-enzyme membrane

    OpenAIRE

    YU, JINGHUA; Ge, Lei; Dai, Ping; Zhang, Congcong

    2010-01-01

    Using glucose oxidase as a model enzyme, a novel rhodanine derivative chemiluminescence biosensor for the determination of glucose was formed based on multilayer-enzyme membrane as receptor, which was assembled via layer-by-layer assembly of sol-gel and glucose oxidase-gold nano-particles inside a glass tube. Compared with the traditional chemiluminescence biosensor, the proposed biosensor had some remarkable advantages, such as good selectivity of substrate, good response performance...

  16. Applications of Nanomaterials in Electrogenerated Chemiluminescence Biosensors

    Directory of Open Access Journals (Sweden)

    Honglan Qi

    2009-01-01

    Full Text Available Electrogenerated chemiluminescence (also called electrochemiluminescence and abbreviated ECL involves the generation of species at electrode surfaces that then undergo electron-transfer reactions to form excited states that emit light. ECL biosensor, combining advantages offered by the selectivity of the biological recognition elements and the sensitivity of ECL technique, is a powerful device for ultrasensitive biomolecule detection and quantification. Nanomaterials are of considerable interest in the biosensor field owing to their unique physical and chemical properties, which have led to novel biosensors that have exhibited high sensitivity and stability. Nanomaterials including nanoparticles and nanotubes, prepared from metals, semiconductor, carbon or polymeric species, have been widely investigated for their ability to enhance the efficiencies of ECL biosensors, such as taking as modification electrode materials, or as carrier of ECL labels and ECL-emitting species. Particularly useful application of nanomaterials in ECL biosensors with emphasis on the years 2004-2008 is reviewed. Remarks on application of nanomaterials in ECL biosensors are also surveyed.

  17. Chemiluminescence and chemiluminescence resonance energy transfer (CRET) aptamer sensors using catalytic hemin/G-quadruplexes.

    Science.gov (United States)

    Liu, Xiaoqing; Freeman, Ronit; Golub, Eyal; Willner, Itamar

    2011-09-27

    The incorporation of hemin into the thrombin/G-quadruplex aptamer assembly or into the ATP/G-quadruplex nanostructure yields active DNAzymes that catalyze the generation of chemiluminescence. These catalytic processes enable the detection of thrombin and ATP with detection limits corresponding to 200 pM and 10 μM, respectively. The conjugation of the antithrombin or anti-ATP aptamers to CdSe/ZnS semiconductor quantum dots (QDs) allowed the detection of thrombin or ATP through the luminescence of the QDs that is powered by a chemiluminescence resonance energy-transfer (CRET) process stimulated by the hemin/G-quadruplex/thrombin complex or the hemin/G-quadruplex/ATP nanostructure, in the presence of luminol/H(2)O(2). The advantages of applying the CRET process for the detection of thrombin or ATP, by the resulting hemin/G-quadruplex DNAzyme structures, are reflected by low background signals and the possibility to develop multiplexed aptasensor assays using different sized QDs. PMID:21866963

  18. Chemiluminescence from alkoxy-substituted acridinium dimethylphenyl ester labels.

    Science.gov (United States)

    Natrajan, Anand; Sharpe, David; Wen, David

    2012-05-01

    Chemiluminescent acridinium dimethylphenyl ester labels are used in automated immunoassays for clinical diagnostics. Light emission from these labels is triggered by alkaline peroxide in the presence of the cationic surfactant cetyltrimethylammonium chloride (CTAC). The surfactant plays a critical role in the chemiluminescence process of these labels by both accelerating their emission kinetics and increasing total light output enabling high throughout and improved assay sensitivity in automated immunoassays. Despite the surfactant's crucial role in the chemiluminescent reaction, no study has investigated how structural perturbations in the acridinium ring could impact the influence of the surfactant. We describe herein the synthesis and properties of three new alkoxy-substituted, acridinium dimethylphenyl esters where the nature of the alkoxy group in the acridinium ring was varied (hydrophobic or hydrophilic). Chemiluminescence measurements of these alkoxy-substituted labels indicate that hydrophilic functional groups in the acridinium ring, in particular sulfobetaine zwitterions, disrupt surfactant-mediated compression of emission times but not enhancement of light yield. These results support the hypothesis that surfactant-mediated effects require the binding of two different reaction intermediates to surfactant aggregates and, that surfactants influence light emission from acridinium esters by two separate mechanisms. Our studies also indicate that preservation of both surfactant effects on acridinium ester chemiluminescence and low non-specific binding of the label can be achieved with a relatively hydrophobic acridinium ring coupled to a hydrophilic phenolic ester leaving group. PMID:22441905

  19. Luminol-potassium permanganate chemiluminescence system for the determination of three anthracycline antibiotics

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Objective To establish a flow-injection chemiluminescence method for the determination of doxorubicin,epirubicin and mitoxantrone and study its reaction mechanism.Methods In alkaline medium,chemiluminescence of luminol-potassium permanganate system could be inhibited obviously by anthracycline antibiotics.Combined with flow-injection technique,a new chemiluminescence method for determining the anthracycline antibiotics was set up.The chemiluminescence mechanism of the luminol-potassium permanganate system w...

  20. Microfluidic device capable of sensing ultrafast chemiluminescence.

    Science.gov (United States)

    Kim, Young-Teck; Ko, Seok Oh; Lee, Ji Hoon

    2009-05-15

    Based on the principle of liquid core waveguide, a novel microfluidic device with micro-scale detection window capable of sensing flashlight emitted from rapid 1,1'-oxalyldi-4-methylimidazole (OD4MI) chemiluminescence (CL) reaction was fabricated. Light emitted from OD4MI CL reaction occurring in the micro-dimensional pentagonal detection window (length of each line segment: 900.0 microm, depth: 50.0 microm) of the microfluidic device with two inlets and one outlet was so bright that it was possible to take an image every 1/30 s at the optimal focusing distance (60 cm) using a commercial digital camera. Peaks obtained using a flow injection analysis (FIA) system with the micro-scale detection window and OD4MI CL detection show excellent resolution and reproducibility without any band-broadening observed in analytical devices having additional reaction channel(s) to measure light generated from slow CL reaction. Maximum height (H(max)) and area (A) of peak, reproducibility and sensitivity observed in the FIA system with the microfluidic device and OD4MI CL detection depends on (1) the mole ratio between bis(2,4,6-trichlorophenyl) oxalate and 4-methyl imidazole yielding OD4MI, (2) the flow rate to mix OD4MI, H(2)O(2) and 1-AP in the detection window of the microfluidic device, and (3) H(2)O(2) concentration. We obtained linear calibration curves with wide dynamic ranges using H(max) and A. The detection limit of 1-AP determined with H(max) and A was as low as 0.05 fmole/injection (signal/background=3.0). PMID:19269463

  1. DNA imaging and quantification using chemi-luminescent probes

    International Nuclear Information System (INIS)

    During this interdisciplinary study we have developed an ultra sensitive and reliable imaging system of DNA labelled by chemiluminescence. Based on a liquid nitrogen cooled CCD, the system achieves sensitivities down to 10 fg/mm2 labelled DNA over a surface area of 25 x 25 cm2 with a sub-millimeter resolution. Commercially available chemi-luminescent - and enhancer molecules are compared and their reaction conditions optimized for best signal-to-noise ratios. Double labelling was performed to verify quantification with radioactive probes. (authors)

  2. Investigation of Chemiluminescence with Electrogenerated Reagents and Its Analytical Application

    Institute of Scientific and Technical Information of China (English)

    章竹君; 李保新; 郑行望

    2003-01-01

    In this paper, studies on chemiluminescence (CL) systems with electrogenerated reagents, including BrO-, ClO-, Br2, [Cu-(HIO6)2]5-, H2O2, Mn3+, Co3+ and Ag2+, are described.The analytical applications of the CL system with electrogenerated reagents are reviewed.

  3. Detection of gamma irradiated pepper and papain by chemiluminescence

    Science.gov (United States)

    Sattar, Abdus; Delincée, H.; Diehl, J. F.

    Chemiluminescence (CL) measurements of black pepper and of papain using luminol and lucigenin reactions were studied. Effects of grinding, irradiation (5-20 kGy) and particle size (750-140 μm) on CL of pepper, and of irradiation (10-30 kGy) on CL of papain, were investigated. All the tested treatments affected the luminescence response in both the luminol and lucigenin reactions; however, the pattern of changes in each case, was inconsistent. Optimum pepper size for maximum luminescence was 560 μm, and optimum irradiation doses were >15 kGy for pepper and >20 kGy for papain. Chemiluminescence may possibly be used as an indicator or irradiation treatment for pepper and papain at a dose of 10 kGy or higher, but further research is needed to establish the reliability of this method.

  4. Reliability Study on Compact and Portable Chemiluminescence Detector

    Directory of Open Access Journals (Sweden)

    Kai-Di Zhang

    2014-11-01

    Full Text Available Two reliability issues on the compact chemiluminescence detector for glucose measurement based on a single planar transparent EWOD (electrowetting-on-dielectrics device are studied. Several dielectrics for lowering the manipulation voltage are investigated and 20-27V is realized. An on-chip heater is designed and manufactured for restore the damage of the hydrophobic surface of the EWOD after chemiluminescence reaction. The measurement of glucose shows the sensitivity of the detector reaches 0.12V/mM and the detection range from 1mM to 20mM with a detection limit of 1mM. Such a detector demonstrates its potential as a portable immuno-detector with prompt response and low cost measurement compared with expensive and bulky traditional instruments.

  5. Nanoparticle-enhanced chemiluminescence in micro-flow injection analysis

    OpenAIRE

    A. Mosayyebi; Karabchevsky, A.; J. S. Wilkinson

    2013-01-01

    Chemiluminescence (CL) detection for biomedical analysis has the principal advantage that no optical source is required so that instrumentation is simple and background radiation is minimised, resulting in high sensitivity. CL has been exploited in a wide range of chemical and biochemical measurements such as enzyme-linked immunoassays (ELISA), DNA sequencing, and for the analysis of biomedical, food and environmental samples [1]. CL is ideally suited to microfluidic flow-injection analysis (...

  6. Quantification of biogenic amines by microchip electrophoresis with chemiluminescence detection

    OpenAIRE

    Zhao, Shulin; Yong HUANG; Shi, Ming; Liu, Yi-Ming

    2009-01-01

    A highly sensitive microchip electrophoresis (MCE) method with chemiluminescence (CL) detection was developed for the determination of biogenic amines including agmatine, epinephrine, dopamine, tyramine, and histamine in human urine samples. To achieve a high assay sensitivity, the targeted analytes were pre-column labeled by a CL tagging reagent, N-(4-aminobutyl)-N-ethylisoluminol (ABEI). ABEI-tagged biogenic amines after MCE separation reacted with hydrogen peroxide in the presence of horse...

  7. Evaluation of the LIAISON Chemiluminescence Immunoassay for Diagnosis of Syphilis▿

    OpenAIRE

    Knight, Carrie S.; Crum, Mary A.; Hardy, Robert W

    2007-01-01

    We report the results of an evaluation of the LIAISON Treponema pallidum-specific assay, a one-step sandwich chemiluminescence immunoassay (CLIA), as a screening test and as a confirmatory test for the diagnosis of syphilis. The assay was compared with the CAPTIA Syphilis-G enzyme immunoassay (EIA) and with a testing algorithm that also included the rapid plasma reagin (RPR) and T. pallidum particle agglutination (PA) assays. As a screening test, the CLIA showed levels of agreement with the E...

  8. [Decrease of singlet oxygen chemiluminescence by the presence of carnosine].

    Science.gov (United States)

    Svachko, A G; Formaziuk, V E; Sergienko, V I

    1990-08-01

    To test antiradical medicines effect the chemical production of singlet oxygen (NaClO + H2O2) was investigated. The quantity singlet oxygen chemiluminescence was decreased in the presence of Japanese Catalin and Chine Baineiting, antirheumatic Voltaren and less strong Finish Catachrome and Carnosine. American Quinax does not possess such an effect. One of the possible starting mechanisms causing different diseases (atherosclerosis, cataract etc.) is destruction of biomembranes by active forms of oxygen.

  9. Chemiluminescence characteristics of cumarin derivatives as blue fluorescers in peroxyoxalate-hydrogen peroxide system

    Science.gov (United States)

    Chaichi, Mohammad Javad; Karami, Ali Reza; Shockravi, Abbas; Shamsipur, Mojtaba

    2003-04-01

    The chemiluminescence characteristics of seven different cumarin derivatives were studied in detail. The fluorescence and chemiluminescence spectra were compared; all cumarins used were found to act as blue fluorescers. The intensity and kinetic parameters for the chemiluminescent systems were evaluated from computer fitting of the resulting intensity-time plots. Among different cumarin derivatives used, 7-amino-4-trifluoromethylcumarin revealed the most promising characteristics as an efficient blue fluorescent emitter.

  10. An overview on flow methods for the chemiluminescence determination of phosphorus

    OpenAIRE

    Morais, Inês P. A.; Tóth, Ildikó V.; Rangel, António O. S. S.

    2005-01-01

    A review on the flow analysis of phosphorus with chemiluminescence detection is presented. A brief discussion of the chemiluminescence principles and applications is given. Particular emphasis is devoted to coupling different flow techniques (flow injection, sequential injection, multicommutation, multisyringe flow injection, multi-pumping) to chemiluminescence detection. Enzymatic and non-enzymatic methods, mostly applied to environmental samples, are summarized and compared in terms of appl...

  11. Electrogenerated chemiluminescence. 58. Ligand-sensitized electrogenerated chemiluminescence in europium labels.

    Science.gov (United States)

    Richter, M M; Bard, A J

    1996-08-01

    The electrochemistry and electrogenerated chemiluminescence (ECL) of a series of europium chelates, cryptates, and mixed-ligand chelate/cryptand complexes were studied. The complexes were of the following general forms:  EuL(4)(-), where L = β-diketonate, a bis-chelating ligand (such as dibenzoylmethide), added as salts (A)EuL(4), where A = tetrabutylammonium ion or piperidinium ion (pipH(+)); Eu(crypt)(3+), where crypt = a cryptand ligand, e.g., 4,7,13,16,21-pentaoxa-1,10-diazabicyclo[8,8,5]tricosane; and Eu(crypt)(L)(2+) for the mixed-ligand systems. ECL was obtained for the chelates and mixed-ligand systems by reducing the complexes at a Pt electrode in the presence of peroxydisulfate in acetonitrile solutions and was attributed to the electron-transfer reaction between the reduced bound ligands and SO(4)(•)(-), followed by intramolecular excitation transfer from the excited ligand orbitals to the metal-centered 4f states. No ECL was observed under the same conditions for the europium complexes incorporating only the cryptand ligands in aqueous solution. The ECL spectra matched the photoluminescence spectra with a narrow emission band observed at 612 nm, corresponding to a metal-centered 4f-4f transition. The ECL efficiencies for the ECL-active species were low, about 10(-)(1)-10(-)(4)% of that of the Ru(bpy)(3)(2+)/S(2)O(8)(2)(-) system under similar conditions. PMID:21619211

  12. Electrogenerated chemiluminescence. 58. Ligand-sensitized electrogenerated chemiluminescence in europium labels

    Energy Technology Data Exchange (ETDEWEB)

    Richter, M.M.; Bard, A.J. [Univ. of Texas, Austin, TX (United States)

    1996-08-01

    The electrochemistry and electrogenerated chemiluminescence (ECL) of a series of europium chelates, cryptates, and mixed-ligand chelate/cryptand complexes were studied. The complexes were of the following general forms: EuL{sub 4}{sup -}, where L = {beta}-diketonate, a bis-chelating ligand (such as dibenzoylmethide), added as salts (A)EuL{sub 4}, where A= tetrabutylammonium ion or piperidinium ion (pipH{sup +}); Eu(crypt){sup 3+}, where crypt = a cryptand ligand, e.g., 4,7,13,16,21-pentaoxa-1,10-diazabicyclo[8,8,5]-tricosa ne; and Eu(crypt)(L){sup 2+} for the mixed-ligand systems. ECL was obtained for the chelates and mixed-ligand systems by reducing the complexes at a Pt electrode in the presence of peroxydisulfate in acetonitrile solutions and was attributed to the electron-transfer reaction between the reduced bound ligands and SO{sub 4}{sup .-}, followed by intramolecular excitation transfer from the excited ligand orbitals to the metal-centered 4f states. No ECL was observed under the same conditions for the europium complexes incorporating only the cryptand ligands in aqueous solution. The ECL spectra matched the photoluminescence spectra with a narrow emission band observed at 612 nm, corresponding to a metal-centered 4f-4f transition. The ECL efficiencies for the ECL-active species were low, about 10{sup -1}-10{sup -4}% of that of the Ru-(bpy){sub 3}{sup 2+}/S{sub 2}O{sub 8}{sup 2-} system under similar conditions. 38 refs., 6 figs., 2 tabs.

  13. Investigation on the spontaneous combustion of refuse-derived fuels during storage using a chemiluminescence technique.

    Science.gov (United States)

    Matunaga, Atsushi; Yasuhara, Akio; Shimizu, Yoshitada; Wakakura, Masahide; Shibamoto, Takayuki

    2008-12-01

    Refuse-derived fuel (RDF), a high-caloric material, is used by various combustion processes, such as power plants, as alternative fuel. Several explosion accidents, however, possibly initiated by the spontaneous combustion of stored RDF, have been reported in Japan. Therefore the spontaneous combustion of RDF prepared from domestic garbage was investigated using chemiluminescence. RDF samples were heated either under air or under nitrogen for 1, 2, or 4 h at 120 or 140 degrees C and then cooled by an air or nitrogen stream. All RDF samples exhibited chemiluminescence. In air-treated RDF samples (heated and cooled by air), chemiluminescence after ageing was shown to be slightly lower than before ageing, whereas in nitrogen-treated samples (both heated and cooled by nitrogen) chemiluminescence decreased significantly after ageing. When nitrogen was replaced with air during aging, however, a sudden increase of chemiluminescence was observed. On the other hand, when cooling was done with air, chemiluminescence increased. Higher chemiluminescence was also observed during high-temperature treatment. Further experiments on cellulose, one of the major components of domestic garbage, exhibited similar chemiluminescence patterns to those of RDF when treated by the same methods as those used for RDF ageing. Chemiluminescence from cellulose increased significantly when the atmospheric gas was changed from nitrogen to air, suggesting that oxygen in the air promoted the formation of hydroperoxide from cellulose. Therefore, it is hypothesized that cellulose plays an important role in the formation of chemiluminescence from RDF. The formation of chemiluminescence indicated that radicals are formed from RDF by oxidation or thermal degradation at room or atmospheric temperatures and may subsequently lead to spontaneous combustion. PMID:19039070

  14. Effect of Saliva on Measurement of Chemiluminescence by a Micro-Reactor Incorporating a Micro-Channel

    OpenAIRE

    Tsukagoshi, Kazuhiko; Fukumoto, Kazuaki; Nakajima, Riichiro; Yamashita, Kenichi; Maeda, Hideaki

    2007-01-01

    Effect of saliva on measurement of chemiluminescence was examined by a micro-reactor incorporating a micro-channel. Sodium hypochlorite and hydrogen peroxide solutions were delivered into a micro-channel developed in a micro-reactor by a syringe pump, providing a laminar flow liquid-liquid interface in the channel and leading to chemiluminescence from singlet oxygen. It was found under certain conditions including saliva that ca. 5% chemiluminescence of the total chemiluminescence was lost in...

  15. Synthesis and properties of differently charged chemiluminescent acridinium ester labels.

    Science.gov (United States)

    Natrajan, Anand; Sharpe, David

    2013-02-14

    Chemiluminescent acridinium dimethylphenyl esters containing N-sulfopropyl groups in the acridinium ring are highly sensitive, hydrophilic labels that are used in automated immunoassays for clinical diagnostics. Light emission from these labels is triggered with alkaline peroxide in the presence of a cationic surfactant. At physiological pH, N-sulfopropyl acridinium esters exist as water adducts that are commonly referred to as pseudobases. Pseudobase formation, which results from addition of water to the zwitterionic N-sulfopropyl acridinium ring, neutralizes the positive charge on the acridinium nitrogen and imparts a net negative charge to the label due to the sulfonate moiety. As a consequence, N-sulfopropyl acridinium ester conjugates of small molecule haptens as well as large molecules such as proteins gain negative charges at neutral pH. In the current study, we describe the synthesis and properties of two new hydrophilic acridinium dimethylphenyl ester labels where the net charge in the labels was altered. In one label, the structure of the hydrophilic N-alkyl group attached to the acridinium ring was changed so that the pseudobase of the label contains no net charge. In the second acridinium ester, two additional negative charges in the form of sulfopropyl groups were added to the acridinium ring to make this label's pseudobase strongly anionic. Chemiluminescence measurements of these labels, as well as their conjugates of an antibody with a neutral pI, indicate that acridinium ester charge while having a modest effect on emission kinetics has little influence on light output. However, our results demonstrate that acridinium ester charge can affect protein pI, apparent chemiluminescence stability and non-specific binding of protein conjugates to microparticles. These results emphasize the need for careful consideration of acridinium ester charge in order to optimize reagent stability and performance in immunoassays. In the current study, we observed that

  16. Sensitive chemiluminescent immunoassay of triclopyr by digital image analysis.

    Science.gov (United States)

    Díaz, Aurora N; Sánchez, Francisco G; Baro, Enrique N; Díaz, Ana F G; Aguilar, Alfonso; Algarra, Manuel

    2012-08-15

    An image based detection of chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA) for the quantification of triclopyr has been developed. The immunoassay was an indirect competitive immunoassay with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (HRP). Chemiluminescence was produced by the luminol/H(2)O(2)/HRP reaction, detected by a monochrome video CCD camera and digitized with an Imagraph IC-PCI frame grabber using a custom program developed in C(++) (Microsoft Visual C(++) 6.0). Two main improvements are reported in the data processing software: the implementation of a circular mesh covering the perimeter of each well, eliminating diffuse light from the neighboring wells, and the use of volume (the integration of light intensity of all pixels that define a well) as an analytical signal instead of CL intensity or area (as usual in commercial plate readers) to improve precision for normalization of the total light output. The standard curve was produced for 0.01-10 ng/L triclopyr. The limit of detection was 0.8 ng/L and the variation coefficient was 3.07% (n=10, P=0.05). PMID:22841045

  17. Selective Detection of Neurotransmitters by Fluorescence and Chemiluminescence Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Ziqiang Wang; Edward S. Yeung

    2001-08-06

    In recent years, luminescence imaging has been widely employed in neurochemical analysis. It has a number of advantages for the study of neuronal and other biological cells: (1) a particular molecular species or cellular constituent can be selectively visualized in the presence of a large excess of other species in a heterogeneous environment; (2) low concentration detection limits can be achieved because of the inherent sensitivity associated with fluorescence and chemiluminescence; (3) low excitation intensities can be used so that long-term observation can be realized while the viability of the specimen is preserved; and (4) excellent spatial resolution can be obtained with the light microscope so subcellular compartments can be identified. With good sensitivity, temporal and spatial resolution, the flux of ions and molecules and the distribution and dynamics of intracellular species can be measured in real time with specific luminescence probes, substrates, or with native fluorescence. A noninvasive detection scheme based on glutamate dehydrogenase (GDH) enzymatic assay combined with microscopy was developed to measure the glutamate release in cultured cells from the central nervous system (CNS). The enzyme reaction is very specific and sensitive. The detection limit with CCD imaging is down to {micro}M levels of glutamate with reasonable response time. They also found that chemiluminescence associated with the ATP-dependent reaction between luciferase and luciferin can be used to image ATP at levels down to 10 nM in the millisecond time scale. Similar imaging experiments should be feasible in a broad spectrum of biological systems.

  18. Chemiluminescence assay for the detection of biological warfare agents

    Energy Technology Data Exchange (ETDEWEB)

    Langry, K; Horn, J

    1999-11-05

    A chemiluminescent homogeneous immunoassay and a hand-size multiassay reader are described that could be used for detecting biological materials. The special feature of the assay is that it employs two different antibodies that each bind to a unique epitope on the same antigen. Each group of epitope-specific antibodies has linked to it an enzyme of a proximal-enzyme pair. One enzyme of the pair utilizes a substrate in high concentration to produce a second substrate required by the second enzyme. This new substrate enables the second enzyme to function. The reaction of the second enzyme is configured to produce light. This chemiluminescence is detected with a charge-coupled device (CCD) camera. The proximal pair enzymes must be in close proximity to one another to allow the second enzyme to react with the product of the first enzyme. This only occurs when the enzyme-linked antibodies are attached to the antigen, whether antigen is a single protein with multiple epitopes or the surface of a cell with a variety of different antigens. As a result of their juxtaposition, the enzymes produce light only in the presence of the biological material. A brief description is given as to how this assay could be utilized in a personal bio-agent detector system.

  19. Chemiluminescent Detection of Enzymatically Produced H2S

    Science.gov (United States)

    Bailey, T. Spencer; Pluth, Michael D.

    2015-01-01

    Hydrogen sulfide (H2S) has emerged as an important biological signaling molecule. To better understand the multifaceted biological roles of H2S, the development of selective and sensitive biocompatible assays for H2S is becoming increasingly important. Motivated by these challenges, our laboratory is developing new methods to further detect and monitor biological H2S. Here, we describe in detail our recent advances in the development and the use of chemiluminescence-based H2S sensors to assist other investigators with use of these chemical tools. We highlight the use of these tools use by displaying their selectivity and high sensitivity toward H2S and provide examples of assays we have developed to detect enzymatically produced H2S. PMID:25725517

  20. Determination of Progesterone Receptor by Chemiluminescent Enzyme Immunoassay

    Institute of Scientific and Technical Information of China (English)

    殷铁军; 顾美皎; 周宜开; 郑文; 胡伟; 芦运萍

    2003-01-01

    A new method of chemiluminescent enzyme immunoassay (CLEIA) was developed and the standard curve and regression equation for determination of progesterone receptor (PR) made. The luminosity of tissue samples was tested and PR level was calculated by the regression equation. Correlation analysis revealed that there was a linear relationship between different concentrations of the standard PR samples and the corresponding values of luminosity: Y = 3748+463. 77X, γ =0. 9958. The values of the luminosity in 38 cases of tumor tissues were determined with the highest being 267. 32 fmol/mg, the lowest 3.69 fmol/mg and the mean 78. 53 fmol/mg. The new method of CLEIA was a stable, creditable,specific and sensitive assay for determination of PR.

  1. Determination of theophylline concentration in serum by chemiluminescent immunoassay

    Institute of Scientific and Technical Information of China (English)

    ZHOU Mei-xia; GUAN Cha-ying; CHEN Guang; XIE Xin-you; WU Sheng-hai

    2005-01-01

    Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum. Methods: To measure the concentration of theophylline (n=122) and evaluate the assay.Results: The linear range of the CLIA method was 0.51~40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 μmol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L). Conclusion: This method is simple, convenient and precise for clinical pharmacokinetics study oftheophylline.

  2. Imaging Electrogenerated Chemiluminescence at Single Gold Nanowire Electrodes.

    Science.gov (United States)

    Wilson, Andrew J; Marchuk, Kyle; Willets, Katherine A

    2015-09-01

    We report electrogenerated chemiluminescence (ECL) generated at single gold nanowire electrodes supported on tin-doped indium oxide. Unlike other single nanoparticle electrochemical characterization techniques, ECL provides a massively parallel direct readout of electrochemical activity on individual nanoparticle electrodes without the need for extrinsic illumination or a scanning electrochemical probe. While ECL is not observed from as-purchased nanowires due to the surfactant layer, by removing the layer and coating the nanowires with a polymer blend, ECL from single nanowire electrodes is readily measured. With an increase in polymer thickness, an increase in ECL image quality and reproducibility over multiple redox cycles is observed. The polymer coating also provides a strategy for stabilizing gold nanoparticle electrodes against complete surface oxidation in aqueous environments. PMID:26267267

  3. The application of automatic chemiluminescence machine in rapid immune detection

    International Nuclear Information System (INIS)

    Objective: To provide high-quality, rapid and dependable result for clinical practice, and give satisfactory service to patients of different economical status by supplementation with other labeling immune examination. With an innovative attitude, we carried out efficient technical reform on ACS180 automatic chemiluminescence machine, making it possible for patients to complete the whole process including examination, check-out, diagnosis and getting drugs. The reported will be issued within an hour, thus a rapid immune detection service was established in out-patients department. Methods: 1. ACS-180 automatic chemiluminescence machine is used based on the principle of chemiluminescence immune methods. 2. The reagents are provided by Ciba-Comig Company of USA, composed of anti acridinium ester antibody of liquid phase and particulate antigen of solid phase wrapped in magnetic powder. 3. Calibration and quality control: high and low concentration are set for each calibration fluid with attached standard curve. Product for quality controlling includes three concentration of low, moderate and high. Results: 1. rapid machine detection for sample: serum is replaced with plasma coagulated by heparin, and comparison among series of methods using serum or plasma suggest no significant difference exists. 2. The problem about fasting detection: chemiluminescence machine measure optical density directly, with the results hardly being influenced by turbidity. But attention should be paid to the treatment of lipid turbid samples. 3. Other innovations: (1) direct placement of sample tube on machine: a cushion is placed on sample plate to transfer sample to machine directly after centrifugation, saving time and reducing the accident in sample transference. (2) for HCG quantification in blood and urine, 'gold criteria' is used firstly in screening to determine approximately the dilution range, with an advantage of saving time and reagent as well as accuracy. (3) we design a

  4. Surface plasmon coupled chemiluminescence during adsorption of oxygen on magnesium surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Hagemann, Ulrich [Interdisciplinary Center for the Analytics on the Nanoscale (ICAN) and Center for Nanointegration Duisburg-Essen (CENIDE), Carl-Benz-Str. 199, 47057 Duisburg (Germany); Nienhaus, Hermann, E-mail: hermann.nienhaus@uni-due.de [Faculty of Physics, University of Duisburg-Essen and Center for Nanointegration Duisburg-Essen (CENIDE), Lotharstr. 1, 47048 Duisburg (Germany)

    2015-12-28

    The dissociative adsorption of oxygen molecules on magnesium surfaces represents a non-adiabatic reaction exhibiting exoelectron emission, chemicurrent generation, and weak chemiluminescence. Using thin film Mg/Ag/p-Si(111) Schottky diodes with 1 nm Mg on a 10-60 nm thick Ag layer as 2π-photodetectors, the chemiluminescence is internally detected with a much larger efficiency than external methods. The chemically induced photoyield shows a maximum for a Ag film thickness of 45 nm. The enhancement is explained by surface plasmon coupled chemiluminescence, i.e., surface plasmon polaritons are effectively excited in the Ag layer by the oxidation reaction and decay radiatively leading to the observed photocurrent. Model calculations of the maximum absorption in attenuated total reflection geometry support the interpretation. The study demonstrates the extreme sensitivity and the practical usage of internal detection schemes for investigating surface chemiluminescence.

  5. Sequential Injection Determination of D-Glucose by Chemiluminescence Using an Open Tubular Immobilised Enzyme Reactor

    DEFF Research Database (Denmark)

    Liu, Xuezhu; Hansen, Elo Harald

    1996-01-01

    A sequential injection analysis system is described that incorporates a nylon tubular reactor containing immobilised glucose oxidase, allowing determination of D-glucose by means of subsequent luminol chemiluminescence detection of the hydrogen peroxide generated in the enzymatic reaction...

  6. Inhibition of human monocyte chemotaxis and chemiluminescence by Pseudomonas aeruginosa elastase

    DEFF Research Database (Denmark)

    Kharazmi, A; Nielsen, H

    1991-01-01

    The in vitro effect of Pseudomonas aeruginosa elastase on human monocyte function was examined. Mononuclear cells isolated from the peripheral blood of healthy individuals were incubated with various concentrations of elastase, and the chemotactic activity and chemiluminescence response of these ......The in vitro effect of Pseudomonas aeruginosa elastase on human monocyte function was examined. Mononuclear cells isolated from the peripheral blood of healthy individuals were incubated with various concentrations of elastase, and the chemotactic activity and chemiluminescence response...

  7. Determination of Ciprofloxacin by Flow Injection Analysis Based on Chemiluminescence System

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A novel flow injection chemiluminescence (CL) method for the determination of ciprofloxacin (CPLX) was reported. The proposed method is based on luminescence produced by KMnO4-Na2S2O4-Tb3+-CPLX chemiluminescence (CL) system. The effects of some critical experimental conditions were discussed and the optimization of working condithe recoveries of real sample analyses were in the range from 110 ± 4 to 104 ± 4.

  8. A highly-sensitive multisubstrate-compatible chemiluminescent immunoassay for human fetuin A

    OpenAIRE

    sprotocols

    2015-01-01

    Authors: Sandeep Kumar Vashist ### Abstract We report a highly-sensitive chemiluminescent immunoassay (CIA) for the detection of human fetuin A (HFA), which is based on the leach-proof covalent crosslinking of anti-HFA capture antibodies on 3-aminopropyltriethoxysilane (APTES)-functionalized 96-well chemiluminescent microtiter plates (CMTP) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysulfosuccinimide. It has more than 3-fold reduced overall assay du...

  9. Optical tomography of fluorophores in dense scattering media based on ultrasound-enhanced chemiluminescence

    International Nuclear Information System (INIS)

    This letter proposes and demonstrates ultrasound-combined optical imaging in dense scattering media. A peroxyoxalate chemiluminescence system that includes fluorophores to chemically excite the pigment is stimulated by ultrasound irradiation with power of less than 0.14 W/cm2. Using focused ultrasound, the chemiluminescence is selectively spatially enhanced, which leads to imaging of the pigment when embedded in a light-scattering medium via scanning of the focal point. The ultrasonically enhanced intensity of the chemiluminescence depends on the base intensity of the chemiluminescence without the applied ultrasound irradiation, which thereby enables quantitative determination of the fluorophore concentration. The authors demonstrate the potential of this method to resolve chemiluminescent targets in a dense scattering medium that is comparable to biological tissue. An image was acquired of a chemiluminescent target that included indocyanine green as the fluorophore embedded at a depth of 20 mm in an Intralipid-10% 200 ml/l solution scattering medium (the reduced scattering coefficient was estimated to be approximately 1.3 mm−1), indicating the potential for expansion of this technique for use in biological applications

  10. Optical tomography of fluorophores in dense scattering media based on ultrasound-enhanced chemiluminescence

    Energy Technology Data Exchange (ETDEWEB)

    Kobayashi, Masaki, E-mail: masaki@tohtech.ac.jp; Kikuchi, Naoto; Sato, Akihiro [Department of Electronics and Intelligent Systems, Tohoku Institute of Technology, Sendai 982-8577 (Japan)

    2015-01-12

    This letter proposes and demonstrates ultrasound-combined optical imaging in dense scattering media. A peroxyoxalate chemiluminescence system that includes fluorophores to chemically excite the pigment is stimulated by ultrasound irradiation with power of less than 0.14 W/cm{sup 2}. Using focused ultrasound, the chemiluminescence is selectively spatially enhanced, which leads to imaging of the pigment when embedded in a light-scattering medium via scanning of the focal point. The ultrasonically enhanced intensity of the chemiluminescence depends on the base intensity of the chemiluminescence without the applied ultrasound irradiation, which thereby enables quantitative determination of the fluorophore concentration. The authors demonstrate the potential of this method to resolve chemiluminescent targets in a dense scattering medium that is comparable to biological tissue. An image was acquired of a chemiluminescent target that included indocyanine green as the fluorophore embedded at a depth of 20 mm in an Intralipid-10% 200 ml/l solution scattering medium (the reduced scattering coefficient was estimated to be approximately 1.3 mm{sup −1}), indicating the potential for expansion of this technique for use in biological applications.

  11. Capillary electrophoresis-chemiluminescence determination of norfloxacin and prulifloxacin

    Energy Technology Data Exchange (ETDEWEB)

    Yang Zhongju; Wang Xiaoli [College of Chemistry, Beijing Normal University, Beijing 100875 (China); Qin Weidong [College of Chemistry, Beijing Normal University, Beijing 100875 (China)], E-mail: qinwd@bnu.edu.cn; Zhao Huichun [College of Chemistry, Beijing Normal University, Beijing 100875 (China)], E-mail: zhaohuichun@bnu.edu.cn

    2008-08-15

    A capillary electrophoresis (CE)-chemiluminescence (CL) method for determining norfloxacin (NFLX) and prulifloxacin (PFLX) was developed based on the enhanced CL intensity of the cerium(IV)-sulfite-fluoroquinolone (FQ) reaction sensitized by terbium(III). The separation was conducted in buffer composed of 20 mM sodium citrate, 4 mM citric acid and 10 mM sodium sulfite at pH 6.1. The CL reagent solution consisted of 2 mM cerium(IV), 4 mM terbium(III) and 1.1 mM hydrochloric acid. NFLX and PFLX were baseline separated within 11 min with detection limits (S/N = 3) of 0.057 and 0.084 {mu}g mL{sup -1}, respectively. The maximum intra- and inter-day relative standard deviations (R.S.D.s) of migration time of the analytes were less than 4.0% and 4.2%, respectively. The proposed method was applied to detect NFLX and PFLX in fortified urine sample and the results were comparable to high-performance liquid chromatography (HPLC)-UV method. Moreover, the high selectivity of the CL detection and the high-separation efficiency of CE render the method the potential of quick analyzing fluoroquinolones in real complex matrix.

  12. Chemiluminescence microarrays in analytical chemistry: a critical review.

    Science.gov (United States)

    Seidel, Michael; Niessner, Reinhard

    2014-09-01

    Multi-analyte immunoassays on microarrays and on multiplex DNA microarrays have been described for quantitative analysis of small organic molecules (e.g., antibiotics, drugs of abuse, small molecule toxins), proteins (e.g., antibodies or protein toxins), and microorganisms, viruses, and eukaryotic cells. In analytical chemistry, multi-analyte detection by use of analytical microarrays has become an innovative research topic because of the possibility of generating several sets of quantitative data for different analyte classes in a short time. Chemiluminescence (CL) microarrays are powerful tools for rapid multiplex analysis of complex matrices. A wide range of applications for CL microarrays is described in the literature dealing with analytical microarrays. The motivation for this review is to summarize the current state of CL-based analytical microarrays. Combining analysis of different compound classes on CL microarrays reduces analysis time, cost of reagents, and use of laboratory space. Applications are discussed, with examples from food safety, water safety, environmental monitoring, diagnostics, forensics, toxicology, and biosecurity. The potential and limitations of research on multiplex analysis by use of CL microarrays are discussed in this review.

  13. Stimulus specific effect of ibuprofen on chemiluminescence of sheep neutrophils

    Energy Technology Data Exchange (ETDEWEB)

    Tahamont, M.V.; Margiotta, M.; Gee, M.H.

    1986-03-05

    The authors have shown that pretreatment with ibuprofen inhibits free radical release from complement stimulated neutrophils. To further examine the effect of ibuprofen on neutrophil free radical release, they stimulated neutrophils with the synthetic peptide, FMLP, phorbol myristate acetate (PMA), or zymosan-activated plasma (ZAP). Pure (>95%), viable (>95%) sheep neutrophils (2 x 10/sup 6/) were placed in HEPES buffer, luminol, drug or vehicle and stimulated in the luminometer with one of the stimuli. The chemiluminescence (CL) response was recorded and the drug treated samples were compared to vehicle treated controls. Ibuprofen had a dose dependent effect on CL in ZAP stimulated neutrophils. At the highest dose (10/sup -2/M) these cells produced only 37 +/- 7% of the CL response observed in the control cells. In contrast, at the same dose, ibuprofen did not significantly attenuate CL seen in FMLP stimulated cells, with these cells producing 79 +/- 7% of the control cells; nor did ibuprofen effect PMA stimulated CL, as these cells produced a CL response that was 85 +/- 8% of the control cells. Ibuprofen appears to have a stimulus specific effect on free radical release in activated neutrophils. It is also apparent that ibuprofen inhibits complement stimulated free radical release by some mechanism independent of its cyclooxygenase inhibitory effect.

  14. Flow injection chemiluminescence determination of lercanidipine based on N-chlorosuccinimide-eosin Y post-chemiluminescence reaction.

    Science.gov (United States)

    Wang, Guowei; Zhao, Fang; Gao, Ying

    2014-12-01

    A novel post-chemiluminescence (PCL) reaction was discovered when lercanidipine was injected into the CL reaction mixture of N-chlorosuccinimide with alkaline eosin Y in the presence of cetyltrimethylammonium bromide (CTAB), where eosin Y was used as the CL reagent and CTAB as the surfactant. Based on this observation, a simple and highly sensitive PCL method combined with a flow injection (FI) technique was developed for the assay of lercanidipine. Under optimum conditions, the CL signal was linearly related to the concentration of lercanidipine in the range 7.0 × 10(-10) to 3.0 × 10(-6)  g/mL with a detection limit of 2.3 × 10(-10) g/mL (3σ). The relative standard deviation (RSD) was 2.1% for 1.0 × 10(-8) g/mL lercanidipine (n = 13). The proposed method had been applied to the estimation of lercanidipine in tablets and human serum samples with satisfactory results. The possible CL mechanism is also discussed briefly.

  15. Development of Chemiluminescent Lateral Flow Assay for the Detection of Nucleic Acids

    Directory of Open Access Journals (Sweden)

    Sam R. Nugen

    2012-01-01

    Full Text Available Rapid, sensitive detection methods are of utmost importance for the identification of pathogens related to health and safety. Herein we report the development of a nucleic acid sequence-based lateral flow assay which achieves a low limit of detection using chemiluminescence. On-membrane enzymatic signal amplification is used to reduce the limit of detection to the sub-femtomol level. To demonstrate this assay, we detected synthetic nucleic acid sequences representative of Trypanosoma mRNA, the causative agent for African sleeping sickness, with relevance in human and animal health in sub-Saharan Africa. The intensity of the chemiluminescent signal was evaluated by using a charge-coupled device as well as a microtiter plate reader. We demonstrated that our lateral flow chemiluminescent assay has a very low limit of detection and is easy to use. The limit of detection was determined to be 0.5 fmols of nucleic acid target.

  16. [Changes in Kinetics of Chemiluminescence of Plasma as a Measure of Systemic Oxidative Stress in Humans].

    Science.gov (United States)

    Sozarukova, M M; Polimova, A M; Proskurnina, E V; Vladimirov, Yu A

    2016-01-01

    Oxidative stress is a pathogenetic factor of many diseases. The control of its level is important for early diagnosis and therapy adjustment. In this work, antioxidant status was estimated in blood plasma. In the system of 2,2'-azo-bis(2-amidinopropane)dihydrochloride-luminol a set of chemiluminescence kinetic curve parameters is proposed for oxidative stress level estimation (the latent period τ(lat) and the increasing of analytical signal ΔI(CL)). Uric acid and albumin were shown as the main components that responsible for changes in chemiluminescence kinetic curve of plasma. Serum albumin undergoes oxidative modification in dose-depend manner under the action of UV irradiation, it causes the enhancement of antioxidant properties. Changes in plasma chemiluminescence kinetics are proposed as a measure of oxidative stress in human body.

  17. Flow injection-chemiluminescence determination of phenol using potassium permanganate and formaldehyde system

    Science.gov (United States)

    Cao, Wei; Mu, Xuemin; Yang, Jinghe; Shi, Wenbo; Zheng, Yongcun

    2007-01-01

    It is found that phenol can react with potassium permanganate in the acidic medium and produce chemiluminescence, which is greatly enhanced by formaldehyde. The optimum conditions for this chemiluminescent reaction are in detail studied using a flow injection system. The experiments indicate that under optimum conditions, the chemiluminescence intensity is linearly related to the concentration of phenol in the range 5.0 × 10 -9 to 1.0 × 10 -6 g mL -1 with a detection limit (3 σ) of 3 × 10 -9 g mL -1. The relative standard deviation is 1.2% for 4.0 × 10 -7 g mL -1 phenol solution in 11 repeated measurements. This method has the advantages of simple operation, fast response and high sensitivity. The method is successfully applied to the determination of phenol in the waste water.

  18. Effect of irradiation on chemiluminescence of EPR pure valcanisate

    International Nuclear Information System (INIS)

    Chemiluminescence of ethylene-propylene pure rubber which is irradiated at various doses was measured. Three methods were attempted to obtain the temperature dependence of the counts of luminescence. In the first method, the relationship between counts of luminescence and time at constant temperature was measured, and the maximum value of counts per unit time is defined as the counts at the temperature (maximum counts method). The relationship between the counts and the passage time was found to be maxwellian after a certain period of time. The extrapolated value to time 0 was defined as the initial count of luminescence in the second method (extrapolate method). In the third method, temperature of the samples was raised stepwise and the counts of each temperature was measured (temperature rising method). Irradiation of 2.7 kGy increases the amount of luminescence remarkably, although the mechanical properties are not affected significantly at the dose. The counts of luminescence does not increase linearly with increasing dose. The counts were found to level off at about 50 kGy. This is because the antioxidant agent which is added during production process of EPR is consumed by irradiation. The results suggests that the rate of autoxidation of the constant temperature depends on whether an antioxidant agent exists or not. The activation energy of the count of CL did not change irrespective of irradiation, and was 82.7 kJ/mol in the extrapolate method. On the other hand, the activation energy for non-irradiated EPR was lower than that of irradiated samples in the maximum counts method, but higher than the values of irradiated samples in the temperature rising method. (author)

  19. Comparison of Chemiluminescence vs. Infrared Techniques for Detection of Fetuin-A in Saliva.

    Science.gov (United States)

    Mathews, Suresh T; Graff, Emily; Judd, Robert L; Kothari, Vishal

    2015-01-01

    The western blotting technique for transfer and detection of proteins, named following the discovery of southern and northern blotting for DNA- and RNA-blotting, respectively, has traditionally relied on the use of X-ray films to capture chemiluminescence. Recent advancements use super-cooled charge coupled devices (CCD) cameras to capture both chemiluminescence and fluorescence images, which exhibit a greater dynamic range compared to traditional X-ray film. Chemiluminescence detected by a CCD camera records photons and displays an image based on the amount of light generated as a result of a dynamic chemical reaction. Fluorescent detection with a CCD camera, on the other hand, is measured in a static state. Despite this advantage, researchers continue to widely use chemiluminescent detection methods due to the generally poor performance of fluorophores in the visible spectrum. Infrared imaging systems offer a solution to the dynamic reactions of chemiluminescence and the poor performance of fluorophores detected in the visible spectrum, by imaging fluorophores in the infrared spectrum. Infrared imaging is static, has a wide linear range, high sensitivity, and reduced autofluorescence and light scatter. A distinct advantage of infrared imaging is the ability to detect two target proteins simultaneously on the same blot which increases accuracy of quantification and comparison, while minimizing the need for stripping and reprobing. Here, we compare the methodology for chemiluminescent (UVP BioChemi) and infrared (UVP Odyssey) detection of salivary total and phosphorylated fetuin-A, a multifunctional protein associated with cardio-metabolic risk, and discuss the advantages and disadvantages of these methodologies.

  20. The source and characteristics of chemiluminescence associated with the oxygenase reaction catalyzed by Mn(2+)-ribulosebisphosphate carboxylase.

    Science.gov (United States)

    Lilley, R M; Riesen, H; Andrews, T J

    1993-07-01

    We confirm the observation of Mogel and McFadden (Mogel, S.N., and McFadden, B. A. (1990) Biochemistry 29, 8333-8337) that ribulosebisphosphate carboxylase/oxygenase (rubisco) exhibits chemiluminescence while catalyzing its oxygenase reaction in the presence of Mn2+. However, our results with the spinach and Rhodospirillum rubrum enzymes differ markedly in the following respects. 1) Chemiluminescence intensity was directly proportional to enzyme concentration and behaved as if representing the rate of oxygenase catalysis. 2) The wavelength spectrum peaked at about 770 nm and extended beyond 810 nm. This seems inconsistent with chemiluminescence generated by simultaneous decay of pairs of singlet O2 molecules. It is consistent with manganese(II) luminescence and we discuss its possible sources. The time course of chemiluminescence (resolution, 0.25 s) was distinctively different for spinach and R. rubrum enzymes during the initial 5 s of catalysis, with the bacterial enzyme exhibiting a pronounced initial "burst." Chemiluminescence by the spinach enzyme responded to substrate concentrations in a manner consistent with known oxygenase properties, exhibiting Michaelis-Menten kinetics with ribulose-1,5-bisphosphate (Km 400 nM). Chemiluminescence required carbamylated enzyme with Mn2+ bound at the active site (activation energy, -57.1 KJ.mol-1). As an indicator of oxygenase activity, chemiluminescence represents an improvement over oxygen electrode measurements in response time and sensitivity by factors of at least 100. PMID:8314755

  1. Improvement on simultaneous determination of chromium species in aqueous solution by ion chromatography and chemiluminescence detection

    DEFF Research Database (Denmark)

    Gammelgaard, Bente; Liao, Y.P.; Jons, O.

    1997-01-01

    A sensitive method for the simultaneous determination of chromium(III) and chromium(VI) was chromatography and chemiluminescence detection. Two Dionex ion-exchange guard columns in series, CG5 and AG7, were used to separate chromium(III) from chromium(VI). Chromium(VI) was reduced by potassium su...

  2. Chemiluminescence: Measuring methods. (Latest citations from the NTIS bibliographic database). Published Search

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-05-01

    The bibliography contains citations concerning chemiluminescence assays. The citations include sample system design, sample collection, measurement techniques, and sensitivity of the instrumentation. Applications in high altitude air pollution studies are emphasized. (Contains 50-250 citations and includes a subject term index and title list.) (Copyright NERAC, Inc. 1995)

  3. Chemiluminescence in the reaction of ozone with 6-methyluracil in aqueous solutions

    Science.gov (United States)

    Zimin, Yu. S.; Khairullina, L. R.; Kutlugil'dina, G. G.; Mustafin, A. G.

    2015-12-01

    Chemiluminescence in the visible part of the spectrum during the oxidation of 6-methyluracil with ozone in aqueous solutions was found. The kinetics of variation of the luminescence intensity was studied in the temperature range 287-333 K, and the activation parameters of the process were determined.

  4. Assay of Cysteine in Human Serum with Quinine-Ce4+ Chemiluminescence System

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A sensitive and selective chemiluminescence (CL) method was developed for the determination of cysteine. This method is based on that the weak CL of cysteine oxidized with cerium (IV) can be greatly enhanced by quinine, and the total cysteine in human serum can be detected through simply diluting with water, showing a simpler analytical characteristic.

  5. A novel chemiluminescent immunoassay for detection of Toxoplasma gondii IgG in human sera.

    Science.gov (United States)

    Holec-Gąsior, Lucyna; Ferra, Bartłomiej; Czechowska, Justyna; Serdiuk, Illia E; Krzymiński, Karol; Kur, Józef

    2016-08-01

    This study describes Toxoplasma gondii IgG chemiluminescent immunoassay (CLIA) based on the use of a novel immunochemical reagents in the form of the conjugates of original acridinium ester (AE) labels attached to antibodies and SAG2-GRA1-ROP1L chimeric antigen and shows that this test is useful for diagnostic purposes.

  6. Synthesis and properties of chemiluminescent acridinium ester labels with fluorous tags.

    Science.gov (United States)

    Natrajan, Anand; Wen, David; Sharpe, David

    2014-06-21

    Acridinium dimethylphenyl esters are highly sensitive chemiluminescent labels that are used in clinical diagnostics. Light emission from these labels is triggered with alkaline peroxide in the presence of the cationic surfactant cetyltrimethylammonium chloride (CTAC). CTAC compresses emission times of these labels to fluorous tags of varying fluorine content and their chemiluminescence in the presence of cationic micelles of CTAC, anionic micelles of sodium perfluorooctanoate (SPFO) as well as mixed micelles of CTAC and SPFO. These studies indicate that in the presence of the mixed micelle system of CTAC and SPFO and at low mole fractions of SPFO, polarity of the mixed micelle interface is lower than that of CTAC leading to a greater enhancement of chemiluminescence for both fluorinated acridinium esters as well as a structurally analogous but non-fluorinated acridinium ester. Chemiluminescence stability of the fluorinated acridinium esters was either comparable to or better than the stability of the non-fluorinated acridinium ester. Non-specific binding to paramagnetic microparticles was higher for fluorinated acridinium esters requiring a surfactant wash to reduce their non-specific binding to the same extent as that observed for the non-fluorinated acridinium ester. PMID:24788381

  7. Acidic Potassium Permanganate Chemiluminescence for the Determination of Antioxidant Potential in Three Cultivars of Ocimum basilicum.

    Science.gov (United States)

    Srivastava, Shivani; Adholeya, Alok; Conlan, Xavier A; Cahill, David M

    2016-03-01

    Ocimum basilicum, a member of the family Lamiaceae, is a rich source of polyphenolics that have antioxidant properties. The present study describes the development and application of an online HPLC-coupled acidic potassium permanganate chemiluminescence assay for the qualitative and quantitative assessment of antioxidants in three cultivars of O. basilicum grown under greenhouse conditions. The chemiluminescence based assay was found to be a sensitive and efficient method for assessment of total and individual compound antioxidant potential. Leaves, flowers and roots were found to be rich reserves of the antioxidant compounds which showed intense chemiluminescence signals. The polyphenolics such as rosmarinic, chicoric, caffeic, p-coumaric, m-coumaric and ferulic acids showed antioxidant activity. Further, rosmarinic acid was found to be the major antioxidant component in water-ethanol extracts. The highest levels of rosmarinic acid was found in the leaves and roots of cultivars "holy green" (14.37; 11.52 mM/100 g DW respectively) followed by "red rubin" (10.02; 10.75 mM/100 g DW respectively) and "subja" (6.59; 4.97 mM/100 g DW respectively). The sensitivity, efficiency and ease of use of the chemiluminescence based assay should now be considered for its use as a primary method for the identification and quantification of antioxidants in plant extracts. PMID:26803763

  8. Soot and chemiluminescence in diesel combustion of bio-derived, oxygenated and reference fuels

    NARCIS (Netherlands)

    Klein-Douwel, R.J.H.; Donkerbroek, A.J.; Vliet, A.P. van; Boot, M.D.; Somers, L.M.T.; Baert, R.S.G.; Dam, N.J.; Meulen, J.J. ter

    2009-01-01

    High-speed imaging, spectroscopy and thermodynamical characterization are applied to an optically accessible, heavy-duty diesel engine in order to compare sooting and chemiluminescence behaviour of bio-derived, oxygenated fuels and various reference fuels. The fuels concerned include the bio-derived

  9. A new label-free strategy for a highly efficient chemiluminescence immunoassay.

    Science.gov (United States)

    Yang, Zhanjun; Cao, Yue; Li, Juan; Wang, Juntao; Du, Dan; Hu, Xiaoya; Lin, Yuehe

    2015-10-01

    A new label-free chemiluminescence (CL) immunoassay method is proposed for the cheap, convenient and sensitive detection of proteins through the co-immobilization of a capture antibody and horseradish peroxidase on a sensing interface. The specific immunoreaction on the interface effectively inhibits the enzymatic CL reaction, thus causing a decrease in CL signals.

  10. Chemiluminescence studies between aqueous phase synthesized mercaptosuccinic acid capped cadmium telluride quantum dots and luminol-H2O2

    Science.gov (United States)

    Kaviyarasan, Kulandaivelu; Anandan, Sambandam; Mangalaraja, Ramalinga Viswanathan; Asiri, Abdullah M.; Wu, Jerry J.

    2016-08-01

    Mercaptosuccinic acid capped Cadmium telluride quantum dots have been successfully synthesized via aqueous phase method. The products were well characterized by a number of analytical techniques, including FT-IR, XRD, HRTEM, and a corrected particle size analysis by the statistical treatment of several AFM measurements. Chemiluminescence experiments were performed to explore the resonance energy transfer between chemiluminescence donor (luminol-H2O2 system) and acceptor CdTe QDs. The combination of such donor and acceptor dramatically reduce the fluorescence while compared to pristine CdTe QDs without any exciting light source, which is due to the occurrence of chemiluminescence resonance energy transfer (CRET) processes.

  11. Chemiluminescence studies between aqueous phase synthesized mercaptosuccinic acid capped cadmium telluride quantum dots and luminol-H2O2.

    Science.gov (United States)

    Kaviyarasan, Kulandaivelu; Anandan, Sambandam; Mangalaraja, Ramalinga Viswanathan; Asiri, Abdullah M; Wu, Jerry J

    2016-08-01

    Mercaptosuccinic acid capped Cadmium telluride quantum dots have been successfully synthesized via aqueous phase method. The products were well characterized by a number of analytical techniques, including FT-IR, XRD, HRTEM, and a corrected particle size analysis by the statistical treatment of several AFM measurements. Chemiluminescence experiments were performed to explore the resonance energy transfer between chemiluminescence donor (luminol-H2O2 system) and acceptor CdTe QDs. The combination of such donor and acceptor dramatically reduce the fluorescence while compared to pristine CdTe QDs without any exciting light source, which is due to the occurrence of chemiluminescence resonance energy transfer (CRET) processes. PMID:27131144

  12. Development and characterization of a microfluidic glucose sensing system based on an enzymatic microreactor and chemiluminescence detection

    NARCIS (Netherlands)

    Moon, B. -U; de Vries, M. G.; Westerink, B. H. C.; Verpoorte, E.

    2012-01-01

    Chemiluminescence detection was developed as an alternative to amperometric detection for glucose analysis in a portable, microfluidics-based continuous glucose monitoring system. Amperometric detection allows easy determination of hydrogen peroxide, a product of the glucose oxidase-catalyzed reacti

  13. Simultaneous Determination of Gold and Platinum by Double Artificial Neural Network Analysis with Flow-injection Chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    Ming Yang LIU; Hai Tao ZHANG; Jun Feng LI; Shu Gui CHEN; Hong Yan WANG

    2006-01-01

    A highly sensitive double artificial neural network (DANN) analysis with flow-injection chemiluminescence (FI-CL) has been developed to simultaneously determine the trace amounts of the gold and platinum in simulated mixed samples, without the boring process.

  14. Advances in the use of acidic potassium permanganate as a chemiluminescence reagent: A review

    Energy Technology Data Exchange (ETDEWEB)

    Adcock, Jacqui L.; Barnett, Neil W.; Barrow, Colin J.; Francis, Paul S., E-mail: psf@deakin.edu.au

    2014-01-07

    Graphical abstract: -- Highlights: •Analytical applications of acidic potassium permanganate chemiluminescence. •Discussion of emitting species and light-producing reaction pathways. •Influence of enhancers such as polyphosphates, formaldehyde and sulfite. •Clinical, forensic, food science, agricultural and environmental applications. -- Abstract: We review the analytical applications of acidic potassium permanganate chemiluminescence published since our previous comprehensive review in mid-2007 to early 2013. This includes a critical evaluation of evidence for the emitting species, the influence of additives such as polyphosphates, formaldehyde, sulfite, thiosulfate, lanthanide complexes and nanoparticles, the development of a generalized reaction mechanism, and the use of this chemistry in pharmaceutical, clinical, forensic, food science, agricultural and environmental applications.

  15. Recent analytical applications of nanoparticle sensitized lucigenin and luminol chemiluminescent reactions

    Indian Academy of Sciences (India)

    S M Wabaidur; Mu Naushad; Z A Alothman

    2012-02-01

    There is an ever-increasing demand for rapid, sensitive, cost effective and selective detection methods for the analysis of many essential compounds.When chemiluminescence has been introduced to analytical chemistry as a detection technique, it has been shown to meet many of these requirements. This method has become a powerful tool for the determination of many compounds. Using this method, low detection limits can be obtained with simple and inexpensive instrumentation. Coupled with flow injection technique the method has become more popular for wider applications. Since many excellent reviews on the chemiluminogenic techniques have appeared in the literature in recent years, the present paper does not intend to cover the exhaustive studies in this area, but will selectively describe the analytical applications of nanoparticle sensitized lucigenin and luminol chemiluminescent reactions and evaluate their recent progress together with our present work.

  16. Chemiluminescence determination of folic acid by a flow injection analysis assembly

    Science.gov (United States)

    Wabaidur, Saikh Mohammad; Alam, Seikh Mafiz; Lee, Sang Hak; Alothman, Zeid Abdullah; Eldesoky, Gaber E.

    2013-03-01

    A flow injection (FI) method is reported for the determination of folic acid by chemiluminescence method. This method is based on the reaction of folic acid with Ru(bipy)32+ and Ce(IV) to produce chemiluminescence. The calibration curve was linear over the range of 2.5 × 10-5-3.1 × 10-7 mol/L with a detection limit of 2.3 × 10-8 mol/L (S/N = 3). The relative standard deviation of 1.0 × 10-6 mol/L folic acid was found 3.5% (n = 11). The influences of potential interfering substances were studied. The recovery was higher than 95.3%. The method was accurate, sensitive, and effective for assay of folic acid. This CL method was successfully applied to the determination of folic acid in pharmaceutical preparations. The mechanism of CL reaction was also studied.

  17. Chemiluminescence Determination of Benzoic Acid Using A Solid-Phase Verdigris Reactor

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    A new chemiluminescence flow system has been developed for sequential determina-tion of benzoic acid based on the reaction of the compound with copper carbonate entrapped in a solid-phase reactor. It was found that the unsaturated complex of Cu(II) and benzoic acid (1:1) has strong catalytic effect on the luminol-H2O2 chemiluminescence reaction. The calibration graph is linear over the range of 0.025 ~ 60 μg/mL of benzoic acid, with a relative standard deviation of less than 3.0 %, and the detection limit is 0.01μg@mL-1. The proposed method was applied to the determination of benzoic acid content in different pharmaceutical formulations.

  18. Chemiluminescent Nanomicelles for Imaging Hydrogen Peroxide and Self-Therapy in Photodynamic Therapy

    Directory of Open Access Journals (Sweden)

    Rui Chen

    2011-01-01

    Full Text Available Hydrogen peroxide is a signal molecule of the tumor, and its overproduction makes a higher concentration in tumor tissue compared to normal tissue. Based on the fact that peroxalates can make chemiluminescence with a high efficiency in the presence of hydrogen peroxide, we developed nanomicelles composed of peroxalate ester oligomers and fluorescent dyes, called peroxalate nanomicelles (POMs, which could image hydrogen peroxide with high sensitivity and stability. The potential application of the POMs in photodynamic therapy (PDT for cancer was also investigated. It was found that the PDT-drug-loaded POMs were sensitive to hydrogen peroxide, and the PDT drug could be stimulated by the chemiluminescence from the reaction between POMs and hydrogen peroxide, which carried on a self-therapy of the tumor without the additional laser light resource.

  19. Determination of glyphosate in foodstuff by one novel chemiluminescence-molecular imprinting sensor

    Science.gov (United States)

    Zhao, Peini; Yan, Mei; Zhang, Congcong; Peng, Ruixue; Ma, Dongsheng; Yu, Jinghua

    2011-05-01

    A novel chemiluminescence (CL) sensor for the determination of glyphosate (GLY) was made up based on molecularly imprinted polymer (MIP). The molecularly imprinted microspheres (MIMs) with a small dimension which possess extremely high surface-to-volume ratio were synthesized using precipitation polymerization with GLY as template. And then the MIMs were modified on glass sheets, which were placed at the bottom of wells of microplate as the recognizer. Subsequently, a highly selective and high throughput chemiluminescence (CL)-molecular imprinting (MI) sensor for detection of GLY was achieved. Influencing factors were investigated and optimized in detail. The method can perform 96 independent measurements sequentially in 10 min and the limit of detection (LOD) for GLY was 0.046 μg mL -1. The relative standard deviation (RSD) for 11 parallel measurements of GLY was 4.68%. The results show that CL-MI sensor can become a useful analytical technology for quick molecular recognition.

  20. Chemiluminescence assay for catechin based on generation of hydrogen peroxide in basic solution

    International Nuclear Information System (INIS)

    We have determined that the catechin group in basic solution efficiently produces hydrogen peroxide; moreover, a highly sensitive analysis methodology was developed to measure catechin employing a peroxalate chemiluminescence detection system. Identification of hydrogen peroxide generated by catechin was determined by ESR as well as peroxalate chemiluminescence using catalase and SOD. As a result, catechin-generated superoxide by electron reduction to dissolved oxygen in basic solution, followed by production of hydrogen peroxide through dismutation reaction. This method could measure several tea catechins, (+)-catechin (CC), (-)-epigallocatechin-3-gallate (EGCg), (-)-epicatechin-3-gallate (ECG) and gallic acid, with measurement range from 10-7 to 10-3 mol/l and sensitivity of 10-8 mol/l. This method was also applied to the determination of total catechin levels in green tea, black tea and roasted green tea

  1. Analysis of Phenolic Compounds in Coke Plant Wastewater by Capillary Zone Electrophoresis with Inhibited Chemiluminescence Detection

    Institute of Scientific and Technical Information of China (English)

    Xiang Dong XU; Yong Gang HU; Ze Yu YANG

    2006-01-01

    A capillary electrophoresis(CE) with on-line inhibited chemiluminescence (CL) detection was firstly used for the simultaneous analysis of benzenediol isomers and phenol. It is based on the quenching effect of benzenediol isomers and phenol on the chemiluminescence reaction of luminol with potassium ferricyanide in sodium hydroxide medium. Under the optimum conditions, the four phenols were baseline separated and detected in less than 10 min.The detection limits (S/N=3) for hydroquinone, resorcinol, catechol and phenol were 2.9×10-8mol/L, 3.7×10-7 mol/L, 8.4×10-8 mol/L and 4.4×10-6 mol/L, respectively. Finally, the presented method has been successfully applied to real sample.

  2. Determination of resveratrol in red wine by solid phase extraction-flow injection chemiluminescence method

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A sensitive flow injection chemiluminescence method has been developed for the detection of resveratrol in red wine based on the fact that resveratrol can greatly enhance chemiluminescence reaction between KMnO4 and HCHO in sulfuric acid medium.Analytes were pre-concentrated on solid sorbents (C18 solid-phase extraction cartridges). Under the optimum conditions, the proposed method allows the measurement of resveratrol over the range of 1.32 × 10-s to 1.32 × 10-5 mol/L with a detection limit of 3.30 × 10-9 mol/L, and the relative standard deviation for 1.32 × 10-5 mol/L resveratrol (n = 11 ) is 3.8%. This method has been successfully applied for the determination of the resveratrol in red wine. Furthermore, the possible reaction mechanism was also discussed.

  3. Effect of external agent on chemiluminescence in bioassay sample - a study

    International Nuclear Information System (INIS)

    Tritium is one of the important radio nuclide contributing about 30-35% of collective dose through internal exposure of plant personnel in Indian PHWRs. Internal dose is monitored by bioassay using liquid scintillation analyzer. There are some external agents that interfere with the tritium counting in urine sample of individuals. These external agents give rise to chemiluminescence in the sample, which may result in wrong interpretation of the counting rates. One such case was studied at Rajasthan Atomic Power Station (RAPS)-3 and 4 in which urine sample of an individual indicated significantly high uptake of tritium whereas the person was not involved in any radioactive job. Investigation revealed that counts due to the sample were caused by chemiluminescence in the urine sample because of homeopathic drug, which the person was taking. (author)

  4. Bioinspired photonic structures by the reflector layer of firefly lantern for highly efficient chemiluminescence

    Science.gov (United States)

    Chen, Linfeng; Shi, Xiaodi; Li, Mingzhu; Hu, Junping; Sun, Shufeng; Su, Bin; Wen, Yongqiang; Han, Dong; Jiang, Lei; Song, Yanlin

    2015-08-01

    Fireflies have drawn considerable attention for thousands of years due to their highly efficient bioluminescence, which is important for fundamental research and photonic applications. However, there are few reports on the reflector layer (RL) of firefly lantern, which contributes to the bright luminescence. Here we presented the detailed microstructure of the RL consisting of random hollow granules, which had high reflectance in the range from 450 nm to 800 nm. Inspired by the firefly lantern, artificial films with high reflectance in the visible region were fabricated using hollow silica microparticles mimicking the structure of the RL. Additionally, the bioinspired structures provided an efficient RL for the chemiluminescence system and could substantially enhance the initial chemiluminescence intensity. The work not only provides new insight into the bright bioluminescence of fireflies, but also is importance for the design of photonic materials for theranostics, detection, and imaging.

  5. Chemiluminescence of curcumin and quenching effect of dimethyl sulfoxide on its peroxyoxalate system

    International Nuclear Information System (INIS)

    The chemiluminescence behavior of the reaction between bis(2,4,6-trichlorophenyl)oxalate (TCPO) and hydrogen peroxide, in the presence of curcumin as fluorophore, has been investigated. Experimental factors such as TCPO, sodium salicylate (SS), hydrogen peroxide and curcumin concentration were optimized. The chemiluminescence signal showed a linear decay while dimethyl sulfoxide (DMSO) was added to the peroxyoxalate (PO-CL) system. The reaction resulted in a Stern-Volmer plot with a Kq value of 7.3x104. The evaluated lower and upper detection limits of measurable concentrations of DMSO are 3.50x10-5 and 1.53x10-4 M, respectively. The PO-CL parameters were estimated by computer fitting of the experimental CL intensity to proper models.

  6. Flow-injection chemiluminescence determination of tryptophan using galangin-potassium permanganate-polyphosphoric acid system

    Institute of Scientific and Technical Information of China (English)

    Hui Chen; Li Li; Min Zhou; Yong Jun Ma

    2008-01-01

    A high sensitive flow-injection chemiluminescence (FI-CL)method for the determination of tryptephan has been developed.The method is based on the chemiluminescence reaction of galangin-potassium perrnanganate-tryptophan in polyphosphoric acid (PPA)media.Under the optimized conditions,tryptophan was determined in the range 0.05-10 μg/mL with the detection limit (30)of 5.0× 10-3 μg/mL.The relative standard deviation (RSD)was 1.0% for 11 replicate determinations of 1.0 μg/mL tryptophan.Three synthetic samples were determined selectively with recoveries in the range from 99.6% to 102.0% in the presence of other amino acids.

  7. Detecting free radicals in sunscreens exposed to UVA radiation using chemiluminescence.

    Science.gov (United States)

    Millington, Keith R; Osmond, Megan J; McCall, Maxine J

    2014-04-01

    One of the current concerns with the application of nanoparticles in sunscreens, and in particular nano-TiO2 and ZnO, is their potential to photogenerate free radicals and reactive oxygen species (ROS) when they absorb ultraviolet wavelengths from sunlight. Free radicals and ROS are known to be associated with UV-induced skin damage and oxidative stress, from which sunscreens are expected to offer significant protection. Here we describe a simple method, based on chemiluminescence emission, for detecting free radicals generated in commercial sunscreens alone, and when applied to various substrates, following exposure to UVA (320-400nm) radiation. This photo-induced chemiluminescence (PICL) technique could be used to optimise sunscreen formulations so as to minimise free radical photogeneration during exposure to sunlight.

  8. Chemiluminescence of curcumin and quenching effect of dimethyl sulfoxide on its peroxyoxalate system

    Energy Technology Data Exchange (ETDEWEB)

    Yari, Abdollah, E-mail: a.yari@ymail.co [Lorestan University, Department of Chemistry, Flakalaflak Street, 68178-17133 Khorramabad (Iran, Islamic Republic of); Saidikhah, Marzieh [Lorestan University, Department of Chemistry, Flakalaflak Street, 68178-17133 Khorramabad (Iran, Islamic Republic of)

    2010-04-15

    The chemiluminescence behavior of the reaction between bis(2,4,6-trichlorophenyl)oxalate (TCPO) and hydrogen peroxide, in the presence of curcumin as fluorophore, has been investigated. Experimental factors such as TCPO, sodium salicylate (SS), hydrogen peroxide and curcumin concentration were optimized. The chemiluminescence signal showed a linear decay while dimethyl sulfoxide (DMSO) was added to the peroxyoxalate (PO-CL) system. The reaction resulted in a Stern-Volmer plot with a K{sub q} value of 7.3x10{sup 4}. The evaluated lower and upper detection limits of measurable concentrations of DMSO are 3.50x10{sup -5} and 1.53x10{sup -4} M, respectively. The PO-CL parameters were estimated by computer fitting of the experimental CL intensity to proper models.

  9. Highly Sensitive Chemiluminescence Detection for PDMS/Glass Micro-chip Electrophoresis

    Institute of Scientific and Technical Information of China (English)

    Xiang Yi HUANG; Jiao Ning WANG; Lin CHEN; Ji Cun REN

    2004-01-01

    This paper described a highly sensitive chemiluminescence detection system for micro-chip electrophoresis (MCE) based on luminol-hydrogen peroxide reaction catalyzed by the metal ions. The micro-chip was composed of poly(dimethylsiloxane) (PDMS) and glass, and was fabricated by micro-machining technology. The surface of channels was dynamically modified by polydimethylacrylamide (PDMA) in order to eliminate unhomogeneous electroosmotic flow (EOF) of the PDMS/glass chip, adsorption of molecules, and improve hydrophobicity on PDMS surface. The detection modes, reagent mix procedures and reaction conditions were optimized and the detection limit of 5 x 10-11 mol/L for cobalt (II) was achieved by MCE with chemiluminescence detection, which was about four orders of magnitude more sensitive than that reported in the reference.

  10. New method for the photo-chemiluminometric determination of benzoylurea insecticides based on acetonitrile chemiluminescence.

    Science.gov (United States)

    Gil García, M D; Martínez Galera, M; Santiago Valverde, R

    2007-03-01

    The viability of tandem photochemical reaction-chemiluminescence detection has been studied for the determination of five benzoylurea insecticides, namely, diflubenzuron, triflumuron, hexaflumuron, lufenuron and flufenoxuron. The 'on-line' photochemical reaction of benzoylurea pesticides provides an enhanced chemiluminescence response of the pesticides during their oxidation by potassium hexacyanoferrate(III) and sodium hydroxide, whose signal increases with the percentage of acetonitrile in the reaction medium. The determination was performed using a photoreactor consisting of a PFA (perfluoroalkoxy) tube reactor coil (5 mx1.6-mm O.D. and 0.8-mm I.D.) and an 8-W xenon lamp. As the yield of the photoderivatization process and the chemiluminescent signals depend on the percentage of acetonitrile, the chromatographic column (a Gemini C18, Phenomenex 150 mmx4.6 mm, 5-microm particle size) was chosen with the aim of using high percentages of this organic solvent in the mobile phase. Previous studies showed that the rate of the chemiluminescent reaction was very fast. Therefore, a modification was carried out in the detector in order to mix the analytes and reactants as near as possible to the measure cell. The optimised method was validated with respect to linearity, precision, limits of detection and quantification accuracy. Under the optimised conditions, linear working range extends three orders of magnitude with the relative standard deviation of intra-day precision below 10% and detection limits between 0.012 and 0.18 microg mL-1, according to the compound. The proposed method has been successfully applied to the determination of benzoylureas in cucumber with good results. PMID:17205265

  11. Quantification of carnosine- related peptides by microchip electrophoresis with chemiluminescence detection

    OpenAIRE

    Zhao, Shulin; Huang, Yong; Shi, Ming; Huang, Junming; Liu, Yi-Ming

    2009-01-01

    A microchip electrophoresis (MCE) method with chemiluminescence (CL) detection was developed for the determination of carnosine-related peptides including carnosine, homocarnosine and anserine in biological samples. A simple integrated MCE-CL system was built to perform the assays. The highly sensitive CL detection was achieved by means of the CL reaction between hydrogen peroxide and N-(4-aminobutyl)- N-ethylisoluminol-tagged peptides in the presence of adenine as a CL enhancer and Co2+ as a...

  12. Development of Chemiluminescent Lateral Flow Assay for the Detection of Nucleic Acids

    OpenAIRE

    Nugen, Sam R.; Catherine Fill; Yuhong Wang

    2012-01-01

    Rapid, sensitive detection methods are of utmost importance for the identification of pathogens related to health and safety. Herein we report the development of a nucleic acid sequence-based lateral flow assay which achieves a low limit of detection using chemiluminescence. On-membrane enzymatic signal amplification is used to reduce the limit of detection to the sub-femtomol level. To demonstrate this assay, we detected synthetic nucleic acid sequences representative of Trypanosoma mRNA, th...

  13. Development of a Flow Injection Manifold for Napropamide Determination by Photo-Induced Chemiluminescence

    OpenAIRE

    Catalá Icardo, Mónica; López Paz, José Luis; Asensio Martín, Víctor

    2012-01-01

    A new, rapid, and simple method is proposed for the determination of the pesticide napropamide by photo-induced chemiluminescence detection coupled with a flow injection analysis (FIA) system. The emission was obtained by oxidation with periodate in basic medium, of the photoproducts generated on-line by UV irradiation (254 nm) of napropamide in acidic SDS (sodium dodecyl sulfate) medium. The flow method, in combination with the solid phase extraction (SPE) performed off-line with C-18 cartri...

  14. Study on Chemiluminescence Reaction between Lucigenin and Four Rare Metal Ions

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The chemiluminescence (CL) reactions between lucigenin and four rare metal reductants were studied systematically using a flow injection system.The results show that the reactions can be used for determination of rare metals.The probable mechanism of the CL reactions involves the reduction of dissolved oxygen to the superoxide radical O-2.by the reductant,followed by the O-2.reacting with alkaline Lu to generate CL.The pathway involves a dioxetane intermediate.

  15. New method for the photo-chemiluminometric determination of benzoylurea insecticides based on acetonitrile chemiluminescence.

    Science.gov (United States)

    Gil García, M D; Martínez Galera, M; Santiago Valverde, R

    2007-03-01

    The viability of tandem photochemical reaction-chemiluminescence detection has been studied for the determination of five benzoylurea insecticides, namely, diflubenzuron, triflumuron, hexaflumuron, lufenuron and flufenoxuron. The 'on-line' photochemical reaction of benzoylurea pesticides provides an enhanced chemiluminescence response of the pesticides during their oxidation by potassium hexacyanoferrate(III) and sodium hydroxide, whose signal increases with the percentage of acetonitrile in the reaction medium. The determination was performed using a photoreactor consisting of a PFA (perfluoroalkoxy) tube reactor coil (5 mx1.6-mm O.D. and 0.8-mm I.D.) and an 8-W xenon lamp. As the yield of the photoderivatization process and the chemiluminescent signals depend on the percentage of acetonitrile, the chromatographic column (a Gemini C18, Phenomenex 150 mmx4.6 mm, 5-microm particle size) was chosen with the aim of using high percentages of this organic solvent in the mobile phase. Previous studies showed that the rate of the chemiluminescent reaction was very fast. Therefore, a modification was carried out in the detector in order to mix the analytes and reactants as near as possible to the measure cell. The optimised method was validated with respect to linearity, precision, limits of detection and quantification accuracy. Under the optimised conditions, linear working range extends three orders of magnitude with the relative standard deviation of intra-day precision below 10% and detection limits between 0.012 and 0.18 microg mL-1, according to the compound. The proposed method has been successfully applied to the determination of benzoylureas in cucumber with good results.

  16. Mechanisms of bioluminescence, chemiluminescence and of their regulation. Progress report, one year period through March 1976

    Energy Technology Data Exchange (ETDEWEB)

    Seliger, H H

    1976-01-01

    Progress is reported on a 10-yr study of the production and role of excited states in biological systems and the mechanisms involved in bioluminescence and chemoluminescence. An hypothesis of the origin of bioluminescence is presented that is based on the mixed function oxygenase reaction. Techniques of absolute measurements of light intensities and spectral composition were applied in studies of bioluminescence of marine dinoflagellates and the chemiluminescence of carcinogenic polycyclic aromatic hydrocarbons as the result of enzymatic hydroxylation. (CH)

  17. Evaluation of nitrogen dioxide chemiluminescence monitors in a polluted urban environment

    OpenAIRE

    Dunlea, E. J.; S. C. Herndon; Nelson, D. D.; Volkamer, R. M.; F. San Martini; Sheehy, P. M.; M. S. Zahniser; Shorter, J. H.; J. C. Wormhoudt; Lamb, B. K.; Allwine, E. J.; J. S. Gaffney; Marley, N. A.; M. Grutter; Marquez, C.

    2007-01-01

    Data from a recent field campaign in Mexico City are used to evaluate the performance of the EPA Federal Reference Method for monitoring ambient concentrations of NO2. Measurements of NO2 from standard chemiluminescence monitors equipped with molybdenum oxide converters are compared with those from Tunable Infrared Laser Differential Absorption Spectroscopy (TILDAS) and Differential Optical Absorption Spectroscopy (DOAS) instruments. A significant...

  18. An Ultrasensitive Chemiluminescence Biosensor for Carcinoembryonic Antigen Based on Autocatalytic Enlargement of Immunogold Nanoprobes

    OpenAIRE

    2012-01-01

    A sensitive flow injection chemiluminescence assay for carcinoembryonic antigen (CEA) detection based on signal amplification with gold nanoparticles (NPs) is reported in the present work. The sandwich system of CEA/anti-CEA/goat-anti-mouse IgG functionalized Au nanoparticles was used as the sensing platform. In order to improve detection sensitivity, a further gold enlargement step was developed based on the autocatalytic Au deposition of gold nanoprobes via the reduction of AuCl4 − to Au0 o...

  19. Development of ultrasensitive direct chemiluminescent enzyme immunoassay for determination of aflatoxin M1 in milk.

    Science.gov (United States)

    Vdovenko, Marina M; Lu, Chuan-Chen; Yu, Feng-Yih; Sakharov, Ivan Yu

    2014-09-01

    A direct competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for detecting aflatoxin M1 (AFM1) was developed. To improve the sensitivity of the assay, a mixture of 3-(10'-phenothiazinyl)-propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORPH) was used to enhance peroxidase-induced CL. The concentrations of the coating anti-AFM1 antibody and the conjugate of AFB1 with horseradish peroxidase the conditions of the chemiluminescent assay were varied to optimise the condition of the chemiluminescent assay. The lower detection limit values and dynamic working range of CL-ELISA of AFM1 were 0.001 ng mL(-1) and 0.002-0.0075 ng mL(-1), respectively. A 20-fold dilution of milk samples prevented a matrix effect of the milk and allowed measurement of AFM1 at concentrations below than the maximum acceptable limit. Values of recovery within and between assays were 81.5-117.6% and 86-110.6%, respectively. The results of using the developed CL-ELISA to analyse samples of six brands of milk that were purchased in Taiwan revealed that AFM1 was absent from all studied samples.

  20. Kinetics analysis of chemiluminescence in discharge-driven HF chemical lasers

    Institute of Scientific and Technical Information of China (English)

    Wei Luo; Shengfu Yuan; Baozhu Yan; Qisheng Lu; Qianjin Zou

    2011-01-01

    The chcinilummescence spectrum in the optical cavity of discharge-driven hydrogen fluoride (HF) chemical laser is measured. The result reveals that the spectra of the helium and fluorine (F) atoms are the major components. Moreover, the green chemiluminescence in the downstream of the optical axis is mostly composed of the 60P20 spectral line of the HF molecule. The analysis shows that, except for the cold pumping reaction, the recombination of the F atoms and the hot pumping reaction also occur in the optical cavity. Due to the hot. Pumping reaction and the optical cavity temperature in a specific range, the 60P20 line becomes the strongest HF molecule in the downstream region of the optical axis. After the hot pumping reaction, the green chcmilum inference always appears in the downstream region of the optical axis when the optical cavity temperature varies in a greater range.%@@ The chemiluminescence spectrum in the optical cavity of discharge-driven hydrogen fluoride(HF) chemical laser is measured.The result reveals that the spectra of the helium and fluorine(F) atoms are the major components.Moreover,the green chemiluminescence in the downstream of the optical axis is mostly composed of the 60P20 spectral line of the HF molecule.

  1. Reagentless chemiluminescence-based fiber optic sensors for regenerative life support in space

    Science.gov (United States)

    Atwater, James E.; Akse, James R.; DeHart, Jeffrey; Wheeler, Richard R., Jr.

    1995-04-01

    The initial feasibility demonstration of a reagentless chemiluminescence based fiber optic sensor technology for use in advanced regenerative life support applications in space and planetary outposts is described. The primary constraints for extraterrestrial deployment of any technology are compatibility with microgravity and hypogravity environments; minimal size, weight, and power consumption; and minimal use of expendables due to the great expense and difficulty inherent to resupply logistics. In the current research, we report the integration of solid state flow through modules for the production of aqueous phase reagents into an integrated system for the detection of important analytes by chemiluminescence, with fiber optic light transmission. By minimizing the need for resupply expendables, the use of solid phase modules makes complex chemical detection schemes practical. For the proof of concept, hydrogen peroxide and glucose were chosen as analytes. The reaction is catalyzed by glucose oxidase, an immobilized enzyme. The aqueous phase chemistry required for sensor operation is implemented using solid phase modules which adjust the pH of the influent stream, catalyze the oxidation of analyte, and provide the controlled addition of the luminophore to the flowing aqueous stream. Precise control of the pH has proven essential for the long-term sustained release of the luminophore. Electrocatalysis is achieved using a controlled potential across gold mesh and gold foil electrodes which undergo periodic polarity reversals. The development and initial characterization of performance of the reagentless fiber optic chemiluminescence sensors are presented in this paper.

  2. A streptavidin functionalized graphene oxide/Au nanoparticles composite for the construction of sensitive chemiluminescent immunosensor

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Zhanjun, E-mail: zjyang@yzu.edu.cn [Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002 (China); Luo, Shufen; Li, Juan; Shen, Juan; Yu, Suhua; Hu, Xiaoya [Key Laboratory of Environmental Material and Environmental Engineering of Jiangsu Province, College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002 (China); Dionysiou, Dionysios D. [Environmental Engineering and Science Program, School of Energy Environmental, Biological and Medical Engineering, University of Cincinnati, Cincinnati, OH 45221-0012 (United States)

    2014-08-11

    Highlights: • A novel streptavidin/GO/AuNPs composite is prepared for immobilizing antibody. • A highly sensitive chemiluminescent immunosensor is constructed for tumor marker. • The immunoassay system shows extremely low detection limit down to picogram level. • This work provides a promising approach for ultrasensitive biosensing applications. - Abstract: In this work, a novel streptavidin functionalized graphene oxide/Au nanoparticles (streptavidin/GO/AuNPs) composite is prepared and for the first time used to construct sensitive chemiluminescent immunosensor for the detection of tumor marker. The streptavidin/GO/AuNPs composite and the immunosensor are characterized using scanning electron microscopy, static water contact angle measurement and electrochemical impedance spectroscopy. The biofunctionalized composite has large reactive surface area and excellent biocompatibility, thus the capture antibody can be efficiently immobilized on its surface based on the highly selective recognition of streptavidin to biotinylated antibody. Using α-fetoprotein (AFP) as a model, the proposed chemiluminescent immunosensor shows a wide linear range from 0.001 to 0.1 ng mL{sup −1} with an extremely low detection limit down to 0.61 pg mL{sup −1}. The resulting AFP immunosensor shows high detection sensitivity, fast assay speed, acceptable detection and fabrication reproducibility, good specificity and stability. The assay results of serum samples with the proposed method are in an acceptable agreement with the reference values. This work provides a promising biofunctionalized nanostructure for sensitive biosensing applications.

  3. Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2O2)-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-250 ng mL·1. The detection limit of CEA was 0.61 ng mL·1. The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.

  4. Insights into the antioxidant activity of some flavones on silver nanoparticles using the chemiluminescence method

    Energy Technology Data Exchange (ETDEWEB)

    Voicescu, Mariana, E-mail: voicescu@icf.ro [Romanian Academy, Institute of Physical Chemistry “Ilie Murgulescu”, Splaiul Independentei 202, 060021 Bucharest (Romania); Nistor, Cristina L. [Polymer Department, National R and D Institute for Chemistry and Petrochemistry ICECHIM, Splaiul Independentei 202, 060021 Bucharest (Romania); Meghea, Aurelia [University POLITEHNICA of Bucharest, Faculty of Applied Chemistry and Materials Sciences, Polizu 1, 78126 Bucharest (Romania)

    2015-01-15

    The work aims to simulate in vitro the effects caused by oxidation of five hydroxyflavones (HF) (some typical models of flavonols), (3-HF, 6-HF, 7-HF, 3,6-diHF and 3,7-diHF) on silver nanoparticles (SNPs) using the chemiluminescent system luminol–hydrogen peroxide, in phosphate buffer, pH 7.4. The contribution of bovine and human serum albumins to the antioxidant activity of the mentioned flavones, and the effect on the SNPs support, in the chemiluminescent system luminol–hydrogen peroxide, has been also investigated. The results are discussed with relevance to the oxidative stress process. - Highlights: • The effects caused by oxidation of five hydroxiflavones (HF) (3-HF, 6-HF, 7-HF, 3,6-diHF and 3,7-diHF) on silver nanoparticles (SNPs) using the chemiluminescent (CL) system luminol–hydrogen peroxide, in phosphate buffer, pH 7.4. • The contribution of bovine and human serum albumins to the antioxidant activity of the mentioned flavones, and the effect on the SNPs support, in the CL system luminol–hydrogen peroxide, are discussed. • The results have relevance to the oxidative stress process.

  5. Selective and Sensitive Chemiluminescence Determination of MCPB: Flow Injection and Liquid Chromatography.

    Science.gov (United States)

    Meseguer-Lloret, Susana; Torres-Cartas, Sagrario; Catalá-Icardo, Mónica; Gómez-Benito, Carmen

    2016-02-01

    Two new chemiluminescence (CL) methods are described for the determination of the herbicide 4-(4-chloro-o-tolyloxy) butyric acid (MCPB). First, a flow injection chemiluminescence (FI-CL) method is proposed. In this method, MCPB is photodegraded with an ultraviolet (UV) lamp and the photoproducts formed provide a great CL signal when they react with ferricyanide in basic medium. Second, a high-performance liquid chromatography chemiluminescence (HPLC-CL) method is proposed. In this method, before the photodegradation and CL reaction, the MCPB and other phenoxyacid herbicides are separated in a C18 column. The experimental conditions for the FI-CL and HPLC-CL methods are optimized. Both methods present good sensitivity, the detection limits being 0.12 µg L(-1) and 0.1 µg L(-1) (for FI-CL and HPLC-CL, respectively) when solid phase extraction (SPE) is applied. Intra- and interday relative standard deviations are below 9.9%. The methods have been satisfactorily applied to the analysis of natural water samples. FI-CL method can be employed for the determination of MCPB in simple water samples and for the screening of complex water samples in a fast, economic, and simple way. The HPLC-CL method is more selective, and allows samples that have not been resolved with the FI-CL method to be solved.

  6. Determination of Epinephrine by Flow Injection Analysis Coupled Ag(Ⅲ) Complex-Luminol Chemiluminescence Detection

    Institute of Scientific and Technical Information of China (English)

    BAI,Jiangbo; SHI,Hongmei; ZHANG,Yingze; TIAN,Dehu; XU,Xiangdong; KANG,Weijun

    2009-01-01

    A new Ag(Ⅲ) complex-luminol chemiluminescent system which was applied to the determination of epinephrine is firstly reported.Based on the enhancing effect of epinephrine on the chemiluminescence reaction of luminol with [Ag(HIO6)2]5- in alkaline solution,a highly sensitive chemiluminescence (CL) detection by flow injection analysis (FIA) was developed for epinephrine.Under the optimum conditions,CL intensity was proportional to concentration of epinephrine in the 1.0× 10 9-1.0×10-7 mol·L-1 range.The limit of detection was 8.0× 10-10mol·L 1 for epinephrine (3σ),with a relative standard deviation (n= 11) of 2.9% for 1.5 × 10 8 mol·L-1 epinephrine.The method validation was done with epinephrine determinations in commercial pharmaceutical products.The mechanism of the reactions was also discussed.

  7. Chemiluminescence evidence supporting the selective role of ligands in the permanganate oxidation of micropollutants.

    Science.gov (United States)

    Roderick, Mark S; Adcock, Jacqui L; Terry, Jessica M; Smith, Zoe M; Parry, Samuel; Linton, Stuart M; Thornton, Megan T; Barrow, Colin J; Francis, Paul S

    2013-10-10

    The selective increase in the oxidation rate of certain organic compounds with permanganate in the presence of environmental "ligands" and reduced species has been ascribed to the different reactivity of the target compounds toward Mn(III), which bears striking similarities to recent independent investigations into the use of permanganate as a chemiluminescence reagent. In spite of the importance of Mn(III) in the light-producing pathway, the dependence of the oxidation mechanism for any given compound on this intermediate could not be determined solely through the emission intensity. However, target compounds susceptible to single-electron oxidation by Mn(III) (such as bisphenol A and triclosan) can be easily distinguished by the dramatic increase in chemiluminescence intensity when a permanganate reagent containing high, stable concentrations of Mn(III) is used. The differences are accentuated under the low pH conditions that favor the chemiluminescence emission due to the greater reactivity of Mn(III) and the greater influence of complexing agents. This study supports the previously postulated selective role of ligands and reducing agents in permanganate oxidations and demonstrates a new approach to explore the chemistry of environmental manganese redox processes. PMID:24050380

  8. Simultaneous determination of chloramphenicol, florfenicol and florfenicol amine in ham sausage with a hybrid chemiluminescent immunoassay.

    Science.gov (United States)

    Tao, Xiaoqi; Jiang, Haiyang; Yu, Xuezhi; Zhu, Jinghui; Wang, Xia; Wang, Zhanhui; Niu, Lanlan; Wu, Xiaoping; Shen, Jianzhong

    2013-01-01

    A novel chemiluminescent immunoassay utilising two types of primary antibodies (murine monoclonal antibody and rabbit polyclonal antibody) and two types of horseradish peroxidase-labelled secondary antibodies was established for simultaneously detecting multiple amphenicol residues in ham sausage. After combining the extract procedure of the target amphenicol into one simplified method, this hybrid chemiluminescent immunoassay could screen chloramphenicol (CAP), florfenicol (FF) and its metabolite florfenicol amine (FFA) at the same time by adding the corresponding secondary antibody. Ham sausage samples were analysed by using this hybrid immunoassay, with LODs of CAP being 0.01 μg kg⁻¹, of FF being 2.8 μg kg⁻¹ and of FFA being 3.0 μg kg⁻¹. The applicability of the proposed method has been validated by determining CAP, FF and FFA in ham sausage samples with satisfactory results. Good recoveries and high correlation with traditional enzyme-linked immunosorbent assay and LC-MS/MS results illustrated that the developed hybrid chemiluminescent immunoassay could screen high-throughput ultra-trace amphenicol residues effectively at one time.

  9. Induction of chemiluminescence of luminol during excitation of NpO2F4OH3- complexes by nitrogen laser (λ = 337.1 nm) radiation

    International Nuclear Information System (INIS)

    Chemiluminescence response of luminol on optical excitation of NpO22+ complexes by nitrogen laser radiation is investigated. Experiments are carried out in solutions containing 42 % of CsF with different ph values. Absorption spectra of NpO2F4OH3- complexes and chemiluminescence spectra of luminol introduced into solutions in the case of different ph are represented

  10. Evaluation of nitrogen dioxide chemiluminescence monitors in a polluted urban environment

    Directory of Open Access Journals (Sweden)

    M. J. Molina

    2007-05-01

    Full Text Available Data from a recent field campaign in Mexico City are used to evaluate the performance of the EPA Federal Reference Method for monitoring the ambient concentrations of NO2. Measurements of NO2 from standard chemiluminescence monitors equipped with molybdenum oxide converters are compared with those from Tunable Infrared Laser Differential Absorption Spectroscopy (TILDAS and Differential Optical Absorption Spectroscopy (DOAS instruments. A significant interference in the chemiluminescence measurement is shown to account for up to 50% of ambient NO2 concentration during afternoon hours. As expected, this interference correlates well with non-NOx reactive nitrogen species (NOz as well as with ambient O3 concentrations, indicating a photochemical source for the interfering species. A combination of ambient gas phase nitric acid and alkyl and multifunctional alkyl nitrates is deduced to be the primary cause of the interference. Observations at four locations at varying proximities to emission sources indicate that the percentage contribution of HNO3 to the interference decreases with time as the air parcel ages. Alkyl and multifunctional alkyl nitrate concentrations are calculated to reach concentrations as high as several ppb inside the city, on par with the highest values previously observed in other urban locations. Averaged over the MCMA-2003 field campaign, the chemiluminescence monitor interference resulted in an average measured NO2 concentration up to 22% greater than that from co-located spectroscopic measurements. Thus, this interference has the potential to initiate regulatory action in areas that are close to non-attainment and may mislead atmospheric photochemical models used to assess control strategies for photochemical oxidants.

  11. Automated determinations of selenium in thermal power plant wastewater by sequential hydride generation and chemiluminescence detection.

    Science.gov (United States)

    Ezoe, Kentaro; Ohyama, Seiichi; Hashem, Md Abul; Ohira, Shin-Ichi; Toda, Kei

    2016-02-01

    After the Fukushima disaster, power generation from nuclear power plants in Japan was completely stopped and old coal-based power plants were re-commissioned to compensate for the decrease in power generation capacity. Although coal is a relatively inexpensive fuel for power generation, it contains high levels (mgkg(-1)) of selenium, which could contaminate the wastewater from thermal power plants. In this work, an automated selenium monitoring system was developed based on sequential hydride generation and chemiluminescence detection. This method could be applied to control of wastewater contamination. In this method, selenium is vaporized as H2Se, which reacts with ozone to produce chemiluminescence. However, interference from arsenic is of concern because the ozone-induced chemiluminescence intensity of H2Se is much lower than that of AsH3. This problem was successfully addressed by vaporizing arsenic and selenium individually in a sequential procedure using a syringe pump equipped with an eight-port selection valve and hot and cold reactors. Oxidative decomposition of organoselenium compounds and pre-reduction of the selenium were performed in the hot reactor, and vapor generation of arsenic and selenium were performed separately in the cold reactor. Sample transfers between the reactors were carried out by a pneumatic air operation by switching with three-way solenoid valves. The detection limit for selenium was 0.008 mg L(-1) and calibration curve was linear up to 1.0 mg L(-1), which provided suitable performance for controlling selenium in wastewater to around the allowable limit (0.1 mg L(-1)). This system consumes few chemicals and is stable for more than a month without any maintenance. Wastewater samples from thermal power plants were collected, and data obtained by the proposed method were compared with those from batchwise water treatment followed by hydride generation-atomic fluorescence spectrometry. PMID:26653491

  12. Toward complete miniaturisation of flow injection analysis systems: microfluidic enhancement of chemiluminescent detection.

    Science.gov (United States)

    Gracioso Martins, Ana M; Glass, Nick R; Harrison, Sally; Rezk, Amgad R; Porter, Nichola A; Carpenter, Peter D; Du Plessis, Johan; Friend, James R; Yeo, Leslie Y

    2014-11-01

    Conventional flow injection systems for aquatic environmental analysis typically comprise large laboratory benchscale equipment, which place considerable constraints for portable field use. Here, we demonstrate the use of an integrated acoustically driven microfluidic mixing scheme to enhance detection of a chemiluminescent species tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate-a common chemiluminescent reagent widely used for the analysis of a wide range of compounds such as illicit drugs, pharmaceuticals, and pesticides-such that rapid in-line quantification can be carried out with sufficient on-chip sensitivity. Specifically, we employ surface acoustic waves (SAWs) to drive intense chaotic streaming within a 100 μL chamber cast in polydimethoxylsiloxane (PDMS) atop a microfluidic chip consisting of a single crystal piezoelectric material. By optimizing the power, duration, and orientation of the SAW input, we show that the mixing intensity of the sample and reagent fed into the chamber can be increased by one to two orders of magnitude, leading to a similar enhancement in the detection sensitivity of the chemiluminescent species and thus achieving a theoretical limit of detection of 0.02 ppb (0.2 nM) of l-proline-a decade improvement over the industry gold-standard and two orders of magnitude more sensitive than that achievable with conventional systems-simply using a portable photodetector and without requiring sample preconcentration. This on-chip microfluidic mixing strategy, together with the integrated miniature photodetector and the possibility for chip-scale microfluidic actuation, then alludes to the attractive possibility of a completely miniaturized platform for portable field-use microanalytical systems. PMID:25275830

  13. Sequential injection chemiluminescence immunoassay for anionic surfactants using magnetic microbeads immobilized with an antibody.

    Science.gov (United States)

    Zhang, Ruiqi; Hirakawa, Koji; Seto, Daisuke; Soh, Nobuaki; Nakano, Koji; Masadome, Takashi; Nagata, Kazumi; Sakamoto, Kazuhira; Imato, Toshihiko

    2005-12-15

    A rapid and sensitive immunoassay for the determination of linear alkylbenzene sulfonates (LAS) is described. The method involves a sequential injection analysis (SIA) system equipped with a chemiluminescence detector and a neodymium magnet. Magnetic beads, to which an anti-LAS monoclonal antibody was immobilized, were used as a solid support in an immunoassay. The introduction, trapping and release of the magnetic beads in the flow cell were controlled by means of a neodymium magnet and adjusting the flow of the carrier solution. The immunoassay was based on an indirect competitive immunoreaction of an anti-LAS monoclonal antibody on the magnetic beads and the LAS sample and horseradish peroxidase (HRP)-labeled LAS, and was based on the subsequent chemiluminscence reaction of HRP with hydrogen peroxide and p-iodophenol, in a luminol solution. The anti-LAS antibody was immobilized on the beads by coupling the antibody with the magnetic beads after activation of a carboxylate moiety on the surface of magnetic beads that had been coated with a polylactic acid film. The antibody immobilized magnetic beads were introduced, and trapped in the flow cell equipped with the neodymium magnet, an LAS solution containing HRP-labeled LAS at constant concentration and the luminol solution were sequentially introduced into the flow cell based on an SIA programmed sequence. Chemiluminescence emission was monitored by means of a photon counting unit located at the upper side of the flow cell by collecting the emitted light with a lens. A typical sigmoid calibration curve was obtained, when the logarithm of the concentration of LAS was plotted against the chemiluminescence intensity using various concentrations of standard LAS samples (0-500ppb) under optimum conditions. The time required for analysis is less than 15min. PMID:18970310

  14. Determination of photoirradiated high polar benzoylureas in tomato by HPLC with luminol chemiluminescence detection.

    Science.gov (United States)

    Galera, M Martínez; García, M D Gil; Valverde, R Santiago

    2008-08-15

    This study reports the first analytical application of luminol chemiluminescence reaction for the sensitive detection of two benzoylurea insecticides (diflubenzuron and triflumuron). Off-line experiments demonstrated that previously irradiated traces of these benzoylurea insecticides largely enhanced the chemiluminescence emission yielded from the oxidation of luminol in methanol:water mixtures, by potassium permanganate in alkaline medium, the enhancement being proportional to the concentration of both pesticides. The two benzoylureas were determined in tomato samples by coupling liquid chromatography with post-column photoderivatization and detection based on this chemiluminescence reaction. Tomato samples were extracted using the QuEChERS method based on extraction with acetonitrile and dispersive solid-phase clean-up using primary and secondary amine (PSA). Interferences due to matrix effect were overcome by using matrix-matched standards. The optimised method was validated with respect to linearity, limits of detection and quantification, precision and accuracy. Under the optimised conditions, calibrations graphs were linear between 0.05 and 0.50 microg mL(-1) for diflubenzuron and between 0.10 and 1.00 microg mL(-1) for triflumuron. Method detection limits were 0.0025 and 0.0131 microg mL(-1) (equivalent to 0.0005 and 0.0026 mg kg(-1)) and quantification limits were 0.05 and 0.10 microg mL(-1) (equivalent to 0.01 and 0.02 mg kg(-1)) for diflubenzuron and triflumuron, respectively. In both cases, quantification limits were lower than the maximum residue levels (MRLs) established by the European legislation. The relative standard deviation of intra-day precision was below 10% and recoveries were between 79.7% and 94.2% for both pesticides. PMID:18656664

  15. Chemiluminescence of phthalhydrazide derivatives in organized media: Interactions with surfactants and cyclodextrins

    Energy Technology Data Exchange (ETDEWEB)

    Maeztu, Raquel [Departamento de Quimica y Edafologia, Facultad de Ciencias, Universidad de Navarra, 31080 Pamplona (Spain); Gonzalez-Gaitano, Gustavo, E-mail: gaitano@unav.e [Departamento de Quimica y Edafologia, Facultad de Ciencias, Universidad de Navarra, 31080 Pamplona (Spain); Tardajos, Gloria [Departamento de Quimica-Fisica I, Facultad de CC. Quimicas, Universidad Complutense, 28040 Madrid (Spain); Stilbs, Peter [Division of Physical Chemistry and Industrial NMR Centre, Department of Chemistry, Royal Institute of Technology, SE-10044, Stockholm (Sweden)

    2011-04-15

    The chemiluminescent oxidation of some phthalhydrazide derivatives, luminol (LUM), isoluminol (ISOL), N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and N-(6-aminohexyl)-N-ethylisoluminol (AHEI), has been carried out in micellar media and in the presence of natural cyclodextrins (CDs), using Co(II) as catalyst and H{sub 2}O{sub 2} as oxidant. The cationic cetyltrimethylammonium bromide (CTAB) and, in a lesser extent, the anionic sodium dodecyl sulfate (SDS) produce quenching in the chemiluminescence (CL) of all the luminophores at concentrations above the cmc, whereas slight enhancements are attained with the non-ionic pentaethylene glycol monododecyl ether (C{sub 12}E{sub 5}). On the contrary, the incorporation of the CDs to the reaction produces a remarkable intensification of the CL. Binding of the luminophores to the macrocycles and the micelles has been studied by Pulsed-Gradient-Spin-Echo-NMR (PGSE-NMR) and fluorescence anisotropy. The cationic CTAB decreases the emission mainly due to charge compensation as a result of the association of the luminophores and the luminescent intermediates to the micelles. The presence of the alkyl substituents of ABEI and AHEI provides an additional hydrophobic contribution to the binding process. SDS quenches this reaction as the micelles retain Co(II) on their anionic layer. The protection and stabilization of these luminophores or their luminescent intermediates provided by the cavities of the CDs make this family of cyclic oligosaccharides much more suitable agents than the surfactants for enhancing the CL in aqueous media for this specific reaction. - Research highlights: Natural cyclodextrins act as boosters of the chemiluminescence of luminol and its derivatives. Cyclodextrins can be successfully employed for improving the possibilities of those applications that make use of the CL of luminol and its derivatives as a detection or quantification method. Ionic and non-ionic surfactants do not either enhance or even quench the

  16. Intercomparison study of NOx passive diffusion tubes with chemiluminescence analysers and evaluation of bias factors

    OpenAIRE

    Sánchez Jiménez, Araceli; Heal, Mathew R.; Beverland, Iain J.

    2011-01-01

    Passive diffusion tubes (PDTs) are an inexpensive and simple method to monitor air pollutants. Numerous studies have investigated the performance of PDTs for NO2 but little attention has been paid to PDTs for NOx. The aim of this study was to evaluate the performance of NOx PDTs in three different urban environments. Duplicate NOx and NO2 PDTs were co-located with chemiluminescence analysers at kerbside, urban centre and background sites in the city of Glasgow for twelve 1-week exposures. PDT...

  17. Chemiluminescence Determination of Molybdenum by on-Line Reduction with a Flow Injection System

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The chemiluminescence (CL) reaction between lucigenin (Lu) and molybdenum (Ⅲ) produced by a Jones reductor was investigated using a flow injection system. On the basis of this, a novel method for the determination of trace amount of molybdenum has been established. The emission intensity was linear with molybdenum concentration in the range of 0.1-1000 ng/mL; the detection limit was 0.02 ng/mL molybdenum; the relative standard deviation was less than 2% for the determination of 0.1 ng/mL molybdenum (n=11). The method has been applied successfully to the analysis of trace molybdenum in water and steel samples.

  18. Fiber-Optic Chemiluminescent Biosensors for Monitoring Aqueous Alcohols and Other Water Quality Parameters

    Science.gov (United States)

    Verostko, Charles E. (Inventor); Atwater, James E. (Inventor); Akse, James R. (Inventor); DeHart, Jeffrey L. (Inventor); Wheeler, Richard R. (Inventor)

    1998-01-01

    A "reagentless" chemiluminescent biosensor and method for the determination of hydrogen peroxide, ethanol and D-glucose in water is disclosed. An aqueous stream is basified by passing it through a solid phase base bed. Luminol is then dissolved in the basified effluent at a controlled rate. Oxidation of the luminol is catalyzed by the target chemical to produce emitted light. The intensity of the emitted light is detected as a measure of the target chemical concentration in the aqueous stream. The emitted light can be transmitted by a fiber optic bundle to a remote location from the aqueous stream for a remote reading of the target chemical concentration.

  19. Flow Injection Analysis of Histidine with Enhanced Electrogenerated Chemiluminescence of Luminol

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    A simple and sensitive flow injection method is presented for the determination of histidine based on its enhancement of electrogenerated chemiluminescence (ECL) of luminol. After optimization of the experimental parameters, the working range for histidine was in 1.0 x 10-6 to 1.0 x 10 -3 mol/L with a detection limit (S/N = 3) of 0.56 μmol/L. The relative standard deviation was 1.6% for 11 measurements of 5 x 10 -5 mol/L histidine solution. The proposed method has been successfully applied to the determination of histidine in real pharmaceutical preparation.

  20. Capillary electrophoresis with direct chemiluminescence detection for the analysis of catecholamines in human urine

    Institute of Scientific and Technical Information of China (English)

    Cheng Quan Wang; Hui Wang; Yan Ming Liu

    2007-01-01

    A rapid and sensitive method for the analysis of three catecholamines by capillary electrophoresis (CE) with direct chemiluminescence (CL) detection is described. The detection limits (S/N = 3) were 1.3 × 10-8 g/mL for isoprenaline,1.0 × 10-8 g/mL for epinephrine and 2.8 × 10-8 g/mL for dopamine. The proposed method was successfully applied to the analysis of catecholamines in urine samples of cigarette smokers and nonsmokers. The results showed that there is a close relation between the release of dopamine in human body fluids and cigarette smoking/nonsmoking.

  1. Investigation of radiaiton defect annealing in LiF by chemiluminescence and thermostimulated luminescence

    International Nuclear Information System (INIS)

    To study radiation defect properties in LiF by simultaneously measured chemi- and thermoluminescence and to prove their regularity, the samples were exposed to gamma-radiation of 60Co with the dose of 1 Mrad/hour. Then the samples were annealed at 100-400 deg C. Dependence of chemi- and thermoluminescence and F-centre concentration on the annealing temperature have been investigated. Those dependences are found to be interrelated. Chemiluminescence intensity change (according to the growth of the annealing temperature for the crystals with a great number of associated electron centres at a high radiation dose) is proved to be caused by the radiation defect clustering

  2. A comparison of chemiluminescent acridinium dimethylphenyl ester labels with different conjugation sites.

    Science.gov (United States)

    Natrajan, Anand; Wen, David

    2015-03-01

    Chemiluminescent acridinium dimethylphenyl esters are highly sensitive labels that are used in automated assays for clinical diagnosis. Light emission from these labels and their conjugates is triggered by treatment with alkaline peroxide. Conjugation of acridinium ester labels is normally done at the phenol. During the chemiluminescent reaction of these acridinium esters, the phenolic ester is cleaved and the light emitting acridone moiety is liberated from its conjugate partner. In the current study, we report the synthesis of three new acridinium esters with conjugation sites at the acridinium nitrogen and compare their properties with that of a conventional acridinium ester with a conjugation site at the phenol. Our study is the first that provides a direct comparison of the emissive properties of acridinium dimethylphenyl esters (free labels and protein conjugates) with different conjugation sites, one where the light emitting acridone remains attached to its conjugate partner versus conventional labeling which results in cleavage of the acridone from the conjugate. Our results indicate that the conjugation at the acridinium nitrogen, which also alters how the acridinium ring and phenol are oriented with respect to the protein surface, has a minimal impact on emission kinetics and emission spectra. However, this mode of conjugation to three different proteins led to a significant increase in light yield which should be useful for improving the assay sensitivity. PMID:25581208

  3. Zwitterionic reagents for labeling, cross-linking and improving the performance of chemiluminescent immunoassays.

    Science.gov (United States)

    Natrajan, Anand; Sharpe, David; Wen, David

    2012-03-01

    Improving reagent performance in immunoassays both to enhance assay sensitivity and to minimize interference are ongoing challenges in clinical diagnostics. We describe herein the syntheses of a new class of hydrophilic reagents containing sulfobetaine zwitterions and their applications. These zwitterionic reagents are potentially useful for improving the properties of immunoassay reagents. We demonstrate for the first time that zwitterion labeling is a general and viable strategy for reducing the non-specific binding of proteins to microparticles and, to improve the aqueous solubility of hydrophobic peptides. We also describe the synthesis of zwitterionic cross-linking reagents and demonstrate their utility for peptide conjugation. In automated, chemiluminescent immunoassays, improved assay performance was observed for a hydrophobic, small analyte (theophylline) using an acridinium ester conjugate with a zwitterionic sulfobetaine linker compared to a hexa(ethylene)glycol linker. Sandwich assay performance for a large analyte (thyroid stimulating hormone) was similar for the two acridinium ester labels. These results indicate that zwitterions are complementary to poly(ethylene)glycol in improving the aqueous solubility and reducing the non-specific binding of chemiluminescent acridinium ester conjugates. PMID:22278720

  4. Streptavidin-functionalized capillary immune microreactor for highly efficient chemiluminescent immunoassay

    Energy Technology Data Exchange (ETDEWEB)

    Yang Zhanjun [State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093 (China); College of Chemistry and Engineering, Yangzhou University, 88 South University Avenue, Yangzhou 225002 (China); Zong Chen [State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093 (China); Ju Huangxian, E-mail: hxju@nju.edu.cn [State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093 (China); Yan Feng, E-mail: yanfeng2007@sohu.com [Jiangsu Institute of Cancer Prevention and Cure, Nanjing 210009 (China)

    2011-11-07

    Highlights: {yields} A novel capillary immune microreactor was proposed for highly efficient flow-through chemiluminescent immunoassay. {yields} The microreactor was prepared by functionalizing capillary inner wall with streptavidin for capture of biotinylated antibody. {yields} The proposed immunoassay method showed wide dynamic range, good reproducibility, stability and practicality. {yields} The microreactor was low-cost and disposable, and possessed several advantages over the conventional immunoreactors. - Abstract: A streptavidin functionalized capillary immune microreactor was designed for highly efficient flow-through chemiluminescent (CL) immunoassay. The functionalized capillary could be used as both a support for highly efficient immobilization of antibody and a flow cell for flow-through immunoassay. The functionalized inner wall and the capture process were characterized using scanning electron microscopy. Compared to conventional packed tube or thin-layer cell immunoreactor, the proposed microreactor showed remarkable properties such as lower cost, simpler fabrication, better practicality and wider dynamic range for fast CL immunoassay with good reproducibility and stability. Using {alpha}-fetoprotein as model analyte, the highly efficient CL flow-through immunoassay system showed a linear range of 3 orders of magnitude from 0.5 to 200 ng mL{sup -1} and a low detection limit of 0.1 ng mL{sup -1}. The capillary immune microreactor could make up the shortcoming of conventional CL immunoreactors and provided a promising alternative for highly efficient flow-injection immunoassay.

  5. Detection of ochratoxin A (OTA) in coffee using chemiluminescence resonance energy transfer (CRET) aptasensor.

    Science.gov (United States)

    Jo, Eun-Jung; Mun, Hyoyoung; Kim, Su-Ji; Shim, Won-Bo; Kim, Min-Gon

    2016-03-01

    We report a chemiluminescence resonance energy transfer (CRET) aptasensor for the detection of ochratoxin A (OTA) in roasted coffee beans. The aptamer sequences used in this study are 5'-DNAzyme-Linker-OTA aptamer-3'-dabcyl. Dabcyl at the end of the OTA aptamer region plays as a quencher in CRET aptasensor. When hemin and OTA are added, the dabcyl-labeled OTA aptamer approaches to the G-quadruplex-hemin complex by formation of the G-quadruplex-OTA complex. The G-quadruplex-hemin complexes possess horseradish peroxidase (HRP)-like activity, and therefore, the HRP-mimicking DNAzyme (HRPzyme) catalyzes peroxidation in the presence of luminol and H2O2. Resonance energy transfer between luminol (donor) and dabcyl (acceptor) enables quenching of chemiluminescence signals. The signal decreases with increasing the concentration of OTA within the range of 0.1-100ngmL(-1) (limit of detection 0.22ngmL(-1)), and the level of recovery of the respective 1ngmL(-1) and 10ngmL(-1) spiked coffee samples was 71.5% and 93.3%. These results demonstrated the potential of the proposed method for OTA analysis in diverse foods.

  6. Chemiluminescence detection of permanganate index (CODMn) by a luminol-KMnO4 based reaction

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    A novel chemiluminescence (CL) system for determination of permanganate index (CODMn) combined with flow injection analysis has been proposed in this study. On the basis of the chemiluminescent reaction of luminol-KMnO4 system, light emission caused by luminol-KMnO4 system was detected by the photomultiplier tube, and its intensity caused by the appearance of KMnO4 after sample digestion was inversely proportional to CODMn. Effects for CODMn determining such as pH, concentrations and interference were investigated in detail. A detection limit of 0.3 mg/L CODMn with a linear range of 0.3-200 mg/L for its theoretical CODMn was obtained under the optimized experimental conditions. The relative standard deviation was 4.3% for 5.0 mg/L CODMn (n = 11). This CL flow system for determining CODMn was simple, rapid, and suitable for automatic analysis. The data obtained by the present method were fairly in good agreement with those obtained by the standard titrimetric method. It has been applied to determine real samples with satisfactory results.

  7. Comparison of chemiluminescence methods for analysis of hydrogen peroxide and hydroxyl radicals

    Energy Technology Data Exchange (ETDEWEB)

    Pehrman, R.; Amme, M. (European Commission, Joint Research Centre, Institute for Transuranium Elements, Karlsruhe, (Germany)); Cachoir, C. (SCK.CEN, Waste and Disposal Unit., Mol (Belgium))

    2010-03-15

    Full text: Disposal of spent nuclear fuel in underground repositories is being considered in many countries and for this purpose understanding of behaviour of radiolysis products is required. To study the effects of alpha radiolysis products of water on oxidation and dissolution of actinides, a method to analyse those products is needed. Chemiluminescence is generally considered a simple, sensitive and reasonably selective method to detect reactive oxygen species on low concentrations. Concentrations of interest for both hydrogen peroxide and hydroxyl radicals are 10-6 to 10-9 M. The aim of this study is to compare various chemiluminescence methods for detecting hydrogen peroxide and hydroxyl radicals. Four methods to analyse hydrogen peroxide were chosen based on the estimated suitability for radiolysis experiments. Two of these use luminol, catalyzed by either mu-peroxidase or hemin, one uses 10-methyl-9-(p-formylphenyl) acridinium carboxylate trifluoromethanesulfonate and one potassium periodate. All methods were tested as batch systems in basic conditions. For hydroxyl radical detection luminophores tested were 3-hydroxyphthalic hydrazide (product of phthalic hydrazide and hydroxyl radical) and rutin. Both methods were tested as batch systems. The results are compared and the applicability of the methods for near-field dissolution studies is discussed. (author)

  8. Correlation between arachidonic acid oxygenation and luminol-induced chemiluminescence in neutrophils: inhibition by diethyldithiocarbamate.

    Science.gov (United States)

    Chabannes, B; Perraut, C; El Habib, R; Moliere, P; Pacheco, Y; Lagarde, M

    1997-04-01

    Neutrophils from allergic subjects were hypersensitive to stimulation by low calcium ionophore concentration (0.15 microM), resulting in an increased formation of leukotriene B4 (LTB4), 5S-hydroxy-6,8,11,14-(E,Z,Z,Z)-eicosatetraenoic acid (5-HETE), and other arachidonic acid metabolites through the 5-lipoxygenase pathway. In parallel, luminol-dependent chemiluminescence was also higher in neutrophils from allergic patients at the basal state and after stimulation by calcium ionophore, revealing an enhancement of radical oxygen species and peroxide production. The activity of glutathione peroxidase, the main enzyme responsible for hydroperoxide reduction, was lowered in these cells. Diethyl-dithiocarbamate (DTC) induced a concentration-dependent decrease in chemiluminescence and arachidonic acid metabolism after neutrophil stimulation. These data show that the elevation of arachidonic acid metabolism in neutrophils from allergic patients is strongly correlated with oxidative status. This elevation may be the consequence of an increased cellular hydroperoxide known to activate 5-lipoxygenase (5-LOX) activity and/or an increased arachidonic acid availability, due either to phospholipase A2 (PLA2) activation or inhibition of arachidonate reesterification into phospholipids. Lowering this oxidative status was associated with a concomitant decrease of this metabolism. Our results suggest that the effect of DTC may be the consequence of an inhibition of peroxyl radical and cellular lipid hydroperoxide production. Thus, DTC may modulate arachidonic acid metabolism in neutrophils by modulating the cellular hydroperoxide level.

  9. Evaluation of Antibacterial Enrofloxacin in Eggs by Matrix Solid Phase Dispersion-Flow Injection Chemiluminescence

    Directory of Open Access Journals (Sweden)

    Xiaocui Duan

    2014-01-01

    Full Text Available The study based on the chemiluminescence (CL reaction of potassium ferricyanide and luminol in sodium hydroxide medium, enrofloxacin (ENRO could dramatically enhance CL intensities and incorporated with matrix solid-phase dispersion (MSPD technique (Florisil used as dispersant, dichloromethane eluted the target compounds. A simple flow injection chemiluminescence (FL-CL method with MSPD technique for determination of ENRO in eggs was described. Under optimal conditions, the CL intensities were linearly related to ENRO concentration ranging from 4.0×10-8 g.L−1 to 5.0×10-5 g.L−1, with a correlation coefficient of 0.9989 and detection limit of 5.0×10-9 g.L−1. The relative standard deviation was 3.6% at an ENRO concentration of 2.0×10-6 g.L−1. Our testing technique can help ensure food safety, and thus, protect public health.

  10. Chemiluminescence of graphene quantum dots and its application to the determination of uric acid

    Energy Technology Data Exchange (ETDEWEB)

    Amjadi, Mohammad, E-mail: amjadi@tabrizu.ac.ir; Manzoori, Jamshid L.; Hallaj, Tooba

    2014-09-15

    We report on the chemiluminescence (CL) of graphene quantum dots (GQDs) induced by direct chemical oxidation. GQDs were prepared by a simple carbonization method and characterized by X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy and Raman spectroscopy. It was found that Ce(IV) could oxidize GQDs to produce a relatively intense CL emission. The mechanism of CL generation was investigated based on the fluorescence and CL emission spectra. It was attributed to the radiative recombination of oxidant-injected holes and thermally excited electrons in the GQDs. In order to show the analytical application potential of GQDs-Ce(IV) CL system, it was applied to the determination of uric acid. Under the optimized conditions, the proposed CL system exhibited excellent analytical performance for determination of uric acid in the range of 1.0×10{sup −6} M–5.0×10{sup −4} M with a limit of detection of 5.0×10{sup −7} M. The method was applied to the determination of uric acid in human plasma and urine samples, with satisfactory results. - Highlights: • Chemiluminescence of graphene quantum dots by direct oxidation was studied. • Intense CL is produced by reaction of graphene quantum dots with Ce(IV). • The new CL system was applied to determination of uric acid in plasma and urine.

  11. Exhaled nitric oxide monitoring by quantum cascade laser: comparison with chemiluminescent and electrochemical sensors

    Science.gov (United States)

    Mandon, Julien; Högman, Marieann; Merkus, Peter J. F. M.; van Amsterdam, Jan; Harren, Frans J. M.; Cristescu, Simona M.

    2012-01-01

    Fractional exhaled nitric oxide (FENO) is considered an indicator in the diagnostics and management of asthma. In this study we present a laser-based sensor for measuring FENO. It consists of a quantum cascade laser (QCL) combined with a multi-pass cell and wavelength modulation spectroscopy for the detection of NO at the sub-part-per-billion by volume (ppbv, 1∶10-9) level. The characteristics and diagnostic performance of the sensor were assessed. A detection limit of 0.5 ppbv was demonstrated with a relatively simple design. The QCL-based sensor was compared with two market sensors, a chemiluminescent analyzer (NOA 280, Sievers) and a portable hand-held electrochemical analyzer (MINO®, Aerocrine AB, Sweden). FENO from 20 children diagnosed with asthma and treated with inhaled corticosteroids were measured. Data were found to be clinically acceptable within 1.1 ppbv between the QCL-based sensor and chemiluminescent sensor and within 1.7 ppbv when compared to the electrochemical sensor. The QCL-based sensor was tested on healthy subjects at various expiratory flow rates for both online and offline sampling procedures. The extended NO parameters, i.e. the alveolar region, airway wall, diffusing capacity, and flux were calculated and showed a good agreement with the previously reported values.

  12. l-Tyrosine Contained in Dietary Supplement by Chemiluminescence Reaction of an Iron-Phthalocyanine Complex

    Directory of Open Access Journals (Sweden)

    Takao Ohtomo

    2012-01-01

    Full Text Available The chemiluminescence (CL signal immediately appeared when a hydrogen peroxide solution was injected into an iron-phthalocyanine tetrasulfonic acid (Fe-PTS aqueous solution. Moreover, the CL intensity of Fe-PTS decreased by adding L-tyrosine. Based on these results, the determination of trace amounts of L-tyrosine was developed using the quenching-chemiluminescence. The calibration curve of L-tyrosine was obtained in the concentration range of 2.0×10−7 M to 2.0×10−5 M. Moreover, the relative standard deviation (RSD was 1.63 % (=5 for 2.0×10−6 M L-tyrosine, and its detection limits (3σ were 1.81×10−7 M. The spike and recovery experiments for L-tyrosine were performed using a soft drink. Furthermore, the determination of L-tyrosine was applied to supplements containing various kinds of amino acids. Each satisfactory relative recovery was obtained at 98 to 102%.

  13. Determination of Biotin in Pharmaceutical Formulations by Potassium Permanganate-luminol-CdTe Nanoparticles Chemiluminescence System

    Institute of Scientific and Technical Information of China (English)

    TRAORE Zoumana Sékou; SU Xing-guang

    2012-01-01

    A sensitive flow-injection chemiluminescence method was developed for the determination of biotin in the pharmaceutical formulations.The affinity between avidin and biotin was used to adsorb biotin on the polystyrene,with subsequent quantification of biotin based on its ability to enhance the chemiluminescence(CL) signal generated by the redox reaction of potassium permanganate-luminol-CdTe nanoparticles CL system.The investigations prove that apart from 3-aminophthalate,the CdTe quantum dots(QDs) play both catalytic and emitter roles.Under optimum conditions,the linear range for the determination of biotin was 0.01-25 ng/mL with a detection limit of 7.3×10-3ng/mL(S/N=3).The relative standard deviation of 5 ng/L biotin was 2.06%(n=7).The proposed method was used to determine the biotin concentration in the pharmaceutical formulations and the recovery was between 96.4% and 104%.The proposed method is simple,convenient,rapid and sensitive.

  14. Flow injection-chemiluminescence determination of dopamine using potassium permanganate and formaldehyde system

    Science.gov (United States)

    Wabaidur, Saikh Mohammad; Alothman, Zeid Abdullah; Alam, Seikh Mafiz; Lee, Sang Hak

    2012-10-01

    A simple and sensitive flow injection-chemiluminescence method for the determination of dopamine has been proposed. The method is based on the enhancing effect of dopamine on the chemiluminescence emission generated by the reaction of potassium permanganate with formaldehyde in an acidic medium. The proposed procedure allows the determination of dopamine over the concentration range of 3.1 × 10-8-1.7 × 10-5 mol/L and with a detection limit of 1.0 × 10-8 mol/L. The linear regression equation was F = 44.4912 + 1.07 × 109 ∗ C (correlation coefficient, r2 = 0.9998). The relative standard deviation is 2.1% for the determination of 1.0 × 10-8 mol/L dopamine (n = 11). The method was successfully applied to the determination of dopamine in pharmaceutical preparation with satisfactory results. The recoveries were found in the range of 96.5-101.3%.

  15. Utility of gold nanoparticles in luminescence determination of trovafloxacin: comparison of chemiluminescence and fluorescence detection.

    Science.gov (United States)

    Alarfaj, Nawal A; El-Tohamy, Maha F

    2015-12-01

    Two novel sensitive sequential injection chemiluminescence analysis and fluorescence methods for trovafloxacin mesylate detection have been developed. The methods were based on the enhancement effect of gold nanoparticles on luminol-ferricyanide-trovafloxacin and europium(III)-trovafloxacin complex systems. The optimum conditions for both detection methods were investigated. The chemiluminescence signal was emitted due to the enhanced effect of gold nanoparticles on the reaction of luminol-ferricyanide-trovafloxacin in an alkaline medium. The response was linear over a concentration range of 1.0 × 10(-9) to 1.0 × 10(-2) mol/L (%RSD = 1.3), (n = 9, r = 0.9991) with a detection limit of 1.7 × 10(-10) mol/L (S/N = 3). The weak fluorescence intensity signal of the oxidation complex of europium(III)-trovafloxacin was strongly enhanced by gold nanoparticles and detected at λex = 330 and λem = 540 nm. Fluorescence detection enabled the determination of trovafloxacin mesylate over a linear range of 1.0 × 10(-8) to 1.0 × 10(-3) mol/L (%RSD = 1.2), (n = 6, r = 0.9993) with a detection limit of 3.3 × 10(-9) mol/L. The proposed methods were successfully applied to the determination of the studied drug in its bulk form and in pharmaceutical preparations. The results were treated statistically and compared with those obtained from other reported methods.

  16. CdS/MoS2 heterojunction-based photoelectrochemical DNA biosensor via enhanced chemiluminescence excitation.

    Science.gov (United States)

    Zang, Yang; Lei, Jianping; Hao, Qing; Ju, Huangxian

    2016-03-15

    This work developed a CdS/MoS2 heterojunction-based photoelectrochemical biosensor for sensitive detection of DNA under the enhanced chemiluminescence excitation of luminol catalyzed by hemin-DNA complex. The CdS/MoS2 photocathode was prepared by the stepwise assembly of MoS2 and CdS quantum dots (QDs) on indium tin oxide (ITO), and achieved about 280% increasing of photocurrent compared to pure CdS QDs electrode due to the formation of heterostructure. High photoconversion efficiency in the photoelectrochemical system was identified to be the rapid spatial charge separation of electron-hole pairs by the extension of electron transport time and electron lifetime. In the presence of target DNA, the catalytic hairpin assembly was triggered, and simultaneously the dual hemin-labeled DNA probe was introduced to capture DNA/CdS/MoS2 modified ITO electrode. Thus the chemiluminescence emission of luminol was enhanced via hemin-induced mimetic catalysis, leading to the physical light-free photoelectrochemical strategy. Under optimized conditions, the resulting photoelectrode was proportional to the logarithm of target DNA concentration in the range from 1 fM to 100 pM with a detection limit of 0.39 fM. Moreover, the cascade amplification biosensor demonstrated high selectivity, desirable stability and good reproducibility, showing great prospect in molecular diagnosis and bioanalysis. PMID:26476013

  17. CdS/MoS2 heterojunction-based photoelectrochemical DNA biosensor via enhanced chemiluminescence excitation.

    Science.gov (United States)

    Zang, Yang; Lei, Jianping; Hao, Qing; Ju, Huangxian

    2016-03-15

    This work developed a CdS/MoS2 heterojunction-based photoelectrochemical biosensor for sensitive detection of DNA under the enhanced chemiluminescence excitation of luminol catalyzed by hemin-DNA complex. The CdS/MoS2 photocathode was prepared by the stepwise assembly of MoS2 and CdS quantum dots (QDs) on indium tin oxide (ITO), and achieved about 280% increasing of photocurrent compared to pure CdS QDs electrode due to the formation of heterostructure. High photoconversion efficiency in the photoelectrochemical system was identified to be the rapid spatial charge separation of electron-hole pairs by the extension of electron transport time and electron lifetime. In the presence of target DNA, the catalytic hairpin assembly was triggered, and simultaneously the dual hemin-labeled DNA probe was introduced to capture DNA/CdS/MoS2 modified ITO electrode. Thus the chemiluminescence emission of luminol was enhanced via hemin-induced mimetic catalysis, leading to the physical light-free photoelectrochemical strategy. Under optimized conditions, the resulting photoelectrode was proportional to the logarithm of target DNA concentration in the range from 1 fM to 100 pM with a detection limit of 0.39 fM. Moreover, the cascade amplification biosensor demonstrated high selectivity, desirable stability and good reproducibility, showing great prospect in molecular diagnosis and bioanalysis.

  18. Simultaneous determination of chromium(III) and chromium(VI) in aqueous solutions by ion chromatography and chemiluminescence detection

    DEFF Research Database (Denmark)

    Gammelgaard, Bente; Jøns, O; Nielsen, B

    1992-01-01

    A method for the simultaneous determination of chromium(iii) and chromium(vi) in a flow system based on chemiluminescence was developed. A Dionex cation-exchange guard column was used to separate chromium(iii) from chromium(vi), and chromium(vi) was reduced by potassium sulfite, whereupon both sp...

  19. Egg-Citing! Isolation of Protoporphyrin IX from Brown Eggshells and Its Detection by Optical Spectroscopy and Chemiluminescence

    Science.gov (United States)

    Dean, Michelle L.; Miller, Tyson A.; Bruckner, Christian

    2011-01-01

    A simple and cost-effective laboratory experiment is described that extracts protoporphyrin IX from brown eggshells. The porphyrin is characterized by UV-vis and fluorescence spectroscopy. A chemiluminescence reaction (peroxyoxalate ester fragmentation) is performed that emits light in the UV region. When the porphyrin extract is added as a fluor…

  20. Investigation of RuBPS-Ce(IV) chemiluminescence reaction and its application in determination of two diuretics

    Energy Technology Data Exchange (ETDEWEB)

    Xi Juan [College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China); Ji Xinghu [College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China); Zhang Shaohong [College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China); Xiang Fan Vocational and Technical College, Xiangfan 441021 (China); Ai Xinping [College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China); He Zhike [College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072 (China)]. E-mail: zhkhe@whu.edu.cn

    2005-06-13

    The chemiluminescence mechanism of tris-(4,7-diphenyl-1,10-phenanthrolinedisulfonic acid)ruthenium(II) (RuBPS)-Ce(IV) system and the effects of two diuretics, hydrochlorothiazide and furosemide, on its chemiluminescence intensity were investigated in detail. It was found that each of the two diuretics could enhance the chemiluminescence emission intensity of RuBPS-Ce(IV) system, based on which, they were sensitively detected by chemiluminescence analysis, respectively. Under the optimum experimental conditions, the linear range and detection limit of hydrochlorothiazide were 2.5 x 10{sup -3} to 6.0 x 10{sup -1} {mu}g ml{sup -1} and 1.0 x 10{sup -3} {mu}g ml{sup -1}, respectively; those of furosemide were 1.0 x 10{sup -2} to 4.0 {mu}g ml{sup -1} and 8.8 x 10{sup -3} {mu}g ml{sup -1}, respectively. The proposed method has been applied to analyze the pharmaceuticals with satisfied results.

  1. Analytically useful blue chemiluminescence from a water-soluble iridium(III) complex containing a tetraethylene glycol functionalised triazolylpyridine ligand.

    Science.gov (United States)

    Smith, Zoe M; Kerr, Emily; Doeven, Egan H; Connell, Timothy U; Barnett, Neil W; Donnelly, Paul S; Haswell, Stephen J; Francis, Paul S

    2016-04-01

    We examine [Ir(df-ppy)2(pt-TEG)](+) as the first highly water soluble, blue-luminescent iridium(III) complex for chemiluminescence detection. Marked differences in selectivity were observed between the new complex and the conventional [Ru(bpy)3](2+) reagent, which will enable this mode of detection to be extended to new areas of application. PMID:26915962

  2. THE OPTIMISATION OF THE SIMULATION OF DIFFUSIONAL TRANSPORT TO A MICROSPHERE ELECTRODE AND ITS APPLICATION TO ELECTROGENERATED CHEMILUMINESCENCE

    OpenAIRE

    Irina B. Svir; Oleinick, Alexander I.; Compton, Richard G.

    2000-01-01

    The application is reported of the three layer finite implicit (3LFI) method for the simulation of electrogenerated chemiluminescence at a microsphere electrode. Two different transformed coordinates in the radial coordinate were employed, a conformal map and an exponentially expanding grid, with aim of optimising the modelling of the diffusion under non steady state conditions.

  3. Microbore liquid chromatography of tertiary amine anticholinergic pharmaceuticals with tris(2,2'-bipyridine)ruthenium(III) chemiluminescence detection.

    Science.gov (United States)

    Holeman, J A; Danielson, N D

    1995-06-01

    The post-column chemiluminescent reaction of six anticholinergic alkaloid compounds with tris(2,2'-bipyridine)ruthenium(III) (Ru(bpy)3(3+)) is applied to microbore high-performance liquid chromatography (HPLC). At flow rates less than 200 microL/min, the capillary mixing cell in which Ru(bpy)3(3+) and the analyte are mixed directly allows for good light detection. In contrast, a diminished signal occurs at these low flow rates with conventional post-column mixing in a tee. Optimal chemiluminescent pH conditions for atropine, scopolamine, dicyclomine, cyclopentolate, cyclobenzaprine, and procyclidine are determined at moderately basic conditions (pH 7 to 9). 2-Butanone is found to be compatible with the chemiluminescent reaction, whereas tetrahydrofuran and propionitrile cause an increase in background noise and a chemiluminescent signal loss. As 2-butanone is more nonpolar than acetonitrile, it assists in the elution of these hydrophobic anticholinergic compounds. Five anticholinergic compounds are resolved successfully with a PRP-1 polymeric column and a slightly basic mobile phase, but a C8 silica column is better suited for the more hydrophobic compounds (cyclobenzaprine, procyclidine, and dicyclomine).

  4. A fluorescent and chemiluminescent difunctional mesoporous silica nanoparticle as a label for the ultrasensitive detection of cancer cells

    Energy Technology Data Exchange (ETDEWEB)

    Tao Liang [Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi' an 710062 (China); Song Chaojun; Sun Yuanjie [Department of Immunology, The Fourth Military Medical University, Xi' an 710032 (China); Li Xiaohua; Li Yunyun [Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi' an 710062 (China); Jin Boquan [Department of Immunology, The Fourth Military Medical University, Xi' an 710032 (China); Zhang Zhujun, E-mail: zhangzj@snnu.edu.cn [Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi' an 710062 (China); Yang Kun, E-mail: yangkunkun@fmmu.edu.cn [Department of Immunology, The Fourth Military Medical University, Xi' an 710032 (China)

    2013-01-25

    Highlights: Black-Right-Pointing-Pointer Difunctional amino mesoporous silica nanoparticles (FCMSN) were synthesized. Black-Right-Pointing-Pointer The fluorescence and chemiluminescence properties of the FCMSN were studied. Black-Right-Pointing-Pointer The NaIO{sub 4} oxidation method was used for modification of the FCMSN. Black-Right-Pointing-Pointer Liver cancer 7721 cell was detected. Black-Right-Pointing-Pointer The specificity affected by FCMSN's amino groups was studied. - Abstract: A new kind of ultrabright fluorescent and chemiluminescent difunctional mesoporous silica nanoparticle (FCMSN) is reported. A luminescent dye, Rhodamine 6G or tris(2,2 Prime -bipyridyl)dichlororuthenium(II) hexahydrate (Rubpy), is doped inside nanochannels of a silica matrix. The hydrophobic groups in the silica matrix avoid the leakage of dye from open channels. The amines groups on the surface of the FCMSN improve the modification performance of the nanoparticle. Because the nanochannels are isolated by a network skeleton of silica, fluorescence quenching based on the inner filter effect of the fluorescent dyes immobilized in nanochannels is weakened effectively. The Quantum Yield of obtained 90 nm silica particles was about 61%. Compared with the fluorescent core-shell nanoparticle, the chemiluminescence reagents can freely enter the nanoparticles to react with fluorescent dyes to create chemiluminescence. The results show that the FCMSN are both fluorescent labels and chemiluminescent labels. In biological applications, the NaIO{sub 4} oxidation method was proven to be superior to the glutaraldehyde method. The amount of amino could affect the specificity of the FCMSN. The fluorescence microscopy imaging demonstrated that the FCMSN is viable for biological applications.

  5. Imaging and high-sensitivity quantification of chemiluminescent labeled DNA-blots

    International Nuclear Information System (INIS)

    The present thesis has for objective the development of both, methods of DNA labeling by chemiluminescence (via the catalytic activity of the enzyme alkaline phosphatase - AP) and an appropriate imaging system. Offering a competitive alternative to the detection of classical radio-labels in molecular-biological experiments of the blotting type, this technique should permit the realization of quantitative studies of gene expression at ultra-high sensitivity necessary in particular for differential-screening experiments. To reach our aim. we separated the project into three different parts. In a first step an imager based on a liquid-nitrogen-cooled CCD coupled to a standard optics (50 mm/fl.2) has been installed and characterized. This system offers a sensitive area of up to 625 cm2, a spatial resolution of 0.3-1 mm (depending on the field of view) and a sensitivity sufficient to detect 10 fg/mm2 labeled DNA. In a second part, the chemiluminescent light-generation process in solution has been investigated to optimize the parameters temperature. pH and concentration of the substrate as well as the enzyme. The substrate offering the highest light yield (CDP-Star in addition with the enhancer EMERALD II) allows quantification of AP down to 10-15 M within a dynamic range of 104 in solution. Finally. preparation, immobilization and detection of AP-labeled DNA probes (via a biotin-streptavidin-biotin-AP bridge) on nylon membranes has been optimized. A linear relation between the light intensities and the amount of DNA was observed in a range of 10 fg/mm2 - 100 pg/mm2. Hybridization of the probes to bacterial cloned target-DNA has been addressed after examination of the best hybridization conditions. Our protocol includes the treatment of a proteinase, which resulted in a significantly lower background on the filter. The results of our investigations suggest that the main conditions for a reliable differential-screening experiment are fulfilled when using chemiluminescent

  6. Chemiluminescence Sensor for the Determination of Hydroxylamine by Electrostatically Immobilizing Luminol and Periodate

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    A novel chemiluminescence(CL) sensor, which can be used for hydroxylamine determination in combination with flow injection analysis, was developed by electrostatically immobilizing luminol and periodate on anion-exchange resin respectively. Hydroxylamine was sensed by its enhancing effect on the weak CL reaction between luminol and periodate, which were eluted from the ion exchange column. The response of the sensor to hydroxylamine was linear in the concentration range of 8.0×10-8-2.0×10-6 mol/L with a detection limit of 4.0×10-8 mol/L hydroxylamine(3σ). The relative standard deviation(RSD) was 2.0% for 9 repetitive determinations at a hydroxylamine concentration of 5.0×10-7 mol/L. The sensor could be reused for over 400 times with a good reproducibility and was used to determine hydroxylamine in wastewater.

  7. Design a New Strategy Based on Nanoparticle-Enhanced Chemiluminescence Sensor Array for Biothiols Discrimination.

    Science.gov (United States)

    Shahrajabian, Maryam; Hormozi-Nezhad, M Reza

    2016-01-01

    Array-based sensor is an interesting approach that suggests an alternative to expensive analytical methods. In this work, we introduce a novel, simple, and sensitive nanoparticle-based chemiluminescence (CL) sensor array for discrimination of biothiols (e.g., cysteine, glutathione and glutathione disulfide). The proposed CL sensor array is based on the CL efficiencies of four types of enhanced nanoparticle-based CL systems. The intensity of CL was altered to varying degrees upon interaction with biothiols, producing unique CL response patterns. These distinct CL response patterns were collected as "fingerprints" and were then identified through chemometric methods, including linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA). The developed array was able to successfully differentiate between cysteine, glutathione and glutathione disulfide in a wide concentration range. Moreover, it was applied to distinguish among the above analytes in human plasma. PMID:27574247

  8. Design a New Strategy Based on Nanoparticle-Enhanced Chemiluminescence Sensor Array for Biothiols Discrimination

    Science.gov (United States)

    Shahrajabian, Maryam; Hormozi-Nezhad, M. Reza

    2016-08-01

    Array-based sensor is an interesting approach that suggests an alternative to expensive analytical methods. In this work, we introduce a novel, simple, and sensitive nanoparticle-based chemiluminescence (CL) sensor array for discrimination of biothiols (e.g., cysteine, glutathione and glutathione disulfide). The proposed CL sensor array is based on the CL efficiencies of four types of enhanced nanoparticle-based CL systems. The intensity of CL was altered to varying degrees upon interaction with biothiols, producing unique CL response patterns. These distinct CL response patterns were collected as “fingerprints” and were then identified through chemometric methods, including linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA). The developed array was able to successfully differentiate between cysteine, glutathione and glutathione disulfide in a wide concentration range. Moreover, it was applied to distinguish among the above analytes in human plasma.

  9. Chemiluminescence and Bioluminescence as an Excitation Source in the Photodynamic Therapy of Cancer: A Critical Review.

    Science.gov (United States)

    Magalhães, Carla M; Esteves da Silva, Joaquim C G; Pinto da Silva, Luís

    2016-08-01

    Photodynamic therapy (PDT) of cancer is known for its limited number of side effects, and requires light, oxygen and photosensitizer. However, PDT is limited by poor penetration of light into deeply localized tissues, and the use of external light sources is required. Thus, researchers have been studying ways to improve the effectiveness of this phototherapy and expand it for the treatment of the deepest cancers, by using chemiluminescent or bioluminescent formulations to excite the photosensitizer by intracellular generation of light. The aim of this Minireview is to give a précis of the most important general chemi-/bioluminescence mechanisms and to analyze several studies that apply them for PDT. These studies have demonstrated the potential of utilizing chemi-/bioluminescence as excitation source in the PDT of cancer, besides combining new approaches to overcome the limitations of this mode of treatment. PMID:27129132

  10. Simultaneous detection of single attoliter droplet collisions by electrochemical and electrogenerated chemiluminescent responses.

    Science.gov (United States)

    Dick, Jeffrey E; Renault, Christophe; Kim, Byung-Kwon; Bard, Allen J

    2014-10-27

    We provide evidence of single attoliter oil droplet collisions at the surface of an ultra-microelectrode (UME) by the observation of simultaneous electrochemical current transients (i-t curves) and electrogenerated chemiluminescent (ECL) transients in an oil/water emulsion. An emulsion system based on droplets of toluene and tri-n-propylamine (2:1 v/v) emulsified with an ionic liquid and suspended in an aqueous continuous phase was formed by ultrasonification. When an ECL luminophore, such as rubrene, is added to the emulsion droplet, stochastic events can be tracked by observing both the current blips from oxidation at the electrode surface and the ECL blips from the follow-up ECL reaction, which produces light. This report provides a means of studying fundamental aspects of electrochemistry using the attoliter oil droplet and offers complementary analytical techniques for analyzing discrete collision events, size distribution of emulsion systems, and individual droplet electroactivity. PMID:25213468

  11. Electrogenerated chemiluminescence of common organic luminophores in water using an emulsion system.

    Science.gov (United States)

    Dick, Jeffrey E; Renault, Christophe; Kim, Byung-Kwon; Bard, Allen J

    2014-10-01

    We describe a method to produce electrogenerated chemiluminescence (ECL) in water using a family of highly hydrophobic polycyclic aromatic hydrocarbon (PAH) luminophores and boron dipyrromethene (BODIPY). This method is based on an oil-in-water emulsion system. Various PAHs (rubrene, 9,10-diphenylanthracene, pyrene, or perylene) and BODIPY were trapped in a toluene and tri-n-propylamine mixed oil-in-water emulsion using an ionic liquid as the supporting electrolyte and emulsifier. ECL was observed for all the aforementioned PAHs and BODIPY, and the rubrene and BODIPY emulsion systems showed adequate light to record an ECL spectrum. ECL was also observed using oxalate as the co-reactant, which was dissolved in the aqueous continuous phase. The emulsions were stable for hours and showed a droplet size distribution that ranged from 275 to 764 nm, in accordance with dynamic light scattering data. PMID:25222019

  12. Chemiluminescence and Bioluminescence as an Excitation Source in the Photodynamic Therapy of Cancer: A Critical Review.

    Science.gov (United States)

    Magalhães, Carla M; Esteves da Silva, Joaquim C G; Pinto da Silva, Luís

    2016-08-01

    Photodynamic therapy (PDT) of cancer is known for its limited number of side effects, and requires light, oxygen and photosensitizer. However, PDT is limited by poor penetration of light into deeply localized tissues, and the use of external light sources is required. Thus, researchers have been studying ways to improve the effectiveness of this phototherapy and expand it for the treatment of the deepest cancers, by using chemiluminescent or bioluminescent formulations to excite the photosensitizer by intracellular generation of light. The aim of this Minireview is to give a précis of the most important general chemi-/bioluminescence mechanisms and to analyze several studies that apply them for PDT. These studies have demonstrated the potential of utilizing chemi-/bioluminescence as excitation source in the PDT of cancer, besides combining new approaches to overcome the limitations of this mode of treatment.

  13. H2S Analysis in Biological Samples Using Gas Chromatography with Sulfur Chemiluminescence Detection

    Science.gov (United States)

    Vitvitsky, Victor; Banerjee, Ruma

    2015-01-01

    Hydrogen sulfide (H2S) is a metabolite and signaling molecule in biological tissues that regulates many physiological processes. Reliable and sensitive methods for H2S analysis are necessary for a better understanding of H2S biology and for the pharmacological modulation of H2S levels in vivo. In this chapter, we describe the use of gas chromatography coupled to sulfur chemiluminescence detection to measure the rates of H2S production and degradation by tissue homogenates at physiologically relevant concentrations of substrates. This method allows separation of H2S from other sulfur compounds and provides sensitivity of detection to ~15 pg (or 0.5 pmol) of H2S per injected sample. PMID:25725519

  14. Chemiluminescence determination of tetracyclines using Fenton system in the presence europium(III) ions

    International Nuclear Information System (INIS)

    A new simple chemiluminescent method for the determination of chlortetracycline (Chlor-TC), oxytetracycline (Oxy-TC) and doxycycline (Doxy-TC) is described. This method is based on the europium(III) emission as a result of the energy transfer process from the excited product of the tetracyclines oxidation to the uncomplexed Eu(III). Under the optimum conditions, calibration graphs were obtained for 4 x 10-7 to 2 x 10-5 mol L-1 of Chlor-TC; 2 x 10-7 to 2 x 10-5 mol L-1 of Oxy-TC and 1 x 10-7 to 3 x 10-5 mol L-1 of Doxy-TC. The method was successfully applied to the determination of these drugs in pharmaceutical and veterinary formulation and honey.

  15. Usage of liquid scintillation counting for detecting the chemiluminescence of cells and its application in medicine

    International Nuclear Information System (INIS)

    The liquid scintillator counting-chemiluminescence (LSC-CL) of mono-photon radiance is a sensitive, handy and high-autoanalytic technique. Through measuring basic CL, dependent CL and maximum phagocytic CL of polymorphonuclear (PMN), we studied best factor levels of the method with orthogonal design [L9 (34)]. The results showed the peak forms changed markedly (inter-group P-4 M). PMN-CL in blood was measured during acute attack of the old patients with chronic bronchitis and the children with pneumonia bronchial. It was suggested that PMN phagocytosis decreased. So the dynamic analysis of maximum phagocytic CL would help us with the deep going clinical researches of the mechanisms of anti-inflammation and injuring by the oxygen free radicals

  16. Chemiluminescence determination of tetracyclines using Fenton system in the presence europium(III) ions

    Energy Technology Data Exchange (ETDEWEB)

    Kaczmarek, Malgorzata [Department of Rare Earths, Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60 - 780 Poznan (Poland); Lis, Stefan, E-mail: blis@amu.edu.pl [Department of Rare Earths, Faculty of Chemistry, Adam Mickiewicz University, Grunwaldzka 6, 60 - 780 Poznan (Poland)

    2009-04-20

    A new simple chemiluminescent method for the determination of chlortetracycline (Chlor-TC), oxytetracycline (Oxy-TC) and doxycycline (Doxy-TC) is described. This method is based on the europium(III) emission as a result of the energy transfer process from the excited product of the tetracyclines oxidation to the uncomplexed Eu(III). Under the optimum conditions, calibration graphs were obtained for 4 x 10{sup -7} to 2 x 10{sup -5} mol L{sup -1} of Chlor-TC; 2 x 10{sup -7} to 2 x 10{sup -5} mol L{sup -1} of Oxy-TC and 1 x 10{sup -7} to 3 x 10{sup -5} mol L{sup -1} of Doxy-TC. The method was successfully applied to the determination of these drugs in pharmaceutical and veterinary formulation and honey.

  17. Developments and Applications of Electrogenerated Chemiluminescence Sensors Based on Micro- and Nanomaterials

    Directory of Open Access Journals (Sweden)

    David T. Pierce

    2008-09-01

    Full Text Available A variety of recent developments and applications of electrogenerated chemiluminescence (ECL for sensors are described. While tris(2,2′-bipyridyl-ruthenium(II and luminol have dominated and continue to pervade the field of ECL-based sensors, recent work has focused on use of these lumophores with micro- and nanomaterials. It has also extended to inherently luminescent nanomaterials, such as quantum dots. Sensor configurations including microelectrode arrays and microfluidics are reviewed and, with the recent trend toward increased use of nanomaterials, special attention has been given to sensors which include thin films, nanoparticles and nanotubes. Applications of ECL labels and examples of label-free sensing that incorporate nanomaterials are also discussed.

  18. Plasmonic luminescent core-shell nanocomposites-enhanced chemiluminescence arising from the decomposition of peroxomonosulfite

    Science.gov (United States)

    Chen, Hui; Xue, Wei; Lu, Chao; Li, Hai-fang; Zheng, Yongzan; Lin, Jin-Ming

    2013-12-01

    A core-shell structure of plasmonic luminescent nanocomposite, Ni@SiO2@FITC@SiO2 (NSFS) combining the stable luminescence of fluorophore with the excellent plasmonic property of metal nanomaterials, has been synthesized through layer-by-layer assembly. The effect of NSFS on the ultraweak chemiluminescence (CL) reaction of hydrogen peroxide (H2O2) and sodium bisulfite (NaHSO3) was explored for the first time. It was found that the CL intensity from the decomposition of peroxomonosulfite was significantly enhanced by NSFS. The mechanism of the nanocomposite-enhanced CL was revealed as the coupling of chemically induced excited states of fluorescein isothiocyanate (FITC) with surface plasmons of Ni nanoparticles based on studies of CL emission spectra, electron spin resonance spectra, extinction spectra and fluorescence spectra. The work sheds new light on the characteristics of the versatile materials and gives us new insight into the optical properties of fluorophores.

  19. Rapid and Sensitive Chemiluminescent Enzyme Immunoassay for the Determination of Neomycin Residues in Milk.

    Science.gov (United States)

    Luo, Peng Jie; Zhang, Jian Bo; Wang, Hua Li; Chen, Xia; Wu, Nan; Zhao, Yun Feng; Wang, Xiao Mei; Zhang, Hong; Zhang, Ji Yue; Zhu, Lei; Jiang, Wen Xiao

    2016-05-01

    Immunoassays greatly contribute to veterinary drug residue analysis. However, there are few reports on detecting neomycin residues by immunoassay. Here, a rapid and sensitive chemiluminescent enzyme immunoassay (CLIEA) was successfully developed for neomycin residue analysis. CLIEA demonstrated good cross-reactivity for neomycin, and the IC50 value was 2.4 ng/mL in buffer. The average recovery range was 88.5%-105.4% for spiked samples (10, 50, and 100 μg/kg), and the coefficient of variation was in the range of 7.5%-14.5%. The limit of detection of CLEIA was 9.4 μg/kg, and this method was compared with the liquid chromatography-tandem mass spectrometry method using naturally contaminated samples, producing a correlation coefficient of >0.95. We demonstrate a reliable CLIEA for the rapid screening of neomycin in milk. PMID:27353712

  20. Determination of Gossypol in Trace Level by Flow Injection Analysis with Chemiluminescence Detection

    Institute of Scientific and Technical Information of China (English)

    Bing Chun XUE; Er Bao LIU

    2006-01-01

    This work reports the single-molecule detection of gossypol by flow injection analysis with chemiluminescence method. The method is based on the reaction of luminol with ferricyanid in sodium hydroxide medium sensitized by gossypol. Under the optimum conditions,the CL intensity is proportional to the concentration of gossypol over the range of 1.11×10-17-2.78×10-16 mol/L in acid solution and 8.00×10-11-7.39×10-8 mol/L in neutral solution with correlation coefficients 0.9983 and 0.9905, respectively. The detection limits is 1.60×l0-18 mol/L (S/N=3). The proposed method has been applied for the determination of the gossypol in cottonseeds and pharmaceutical formulations with satisfactory results.

  1. Chemiluminescence Sensor for the Determination of Hydroxylamine by Electrostatically Immobilizing Luminol and Periodate

    Institute of Scientific and Technical Information of China (English)

    ZHANGSi-Chun; ZHOUGuo-jun; JUHuang-xian

    2003-01-01

    A novel chemiluminescence(CL)sensor,which can be used for hydroxylamine determination in combination with flow injection analysis.was developed by electrostatically immobilizing luminol and periodate on anion-exchange resin respectively.Hydroxylamine was sensed by its enhacing effect on the weak CL reaction between luminol and periodate,which were eluted from the ion exchange column.the response of the sensor to hydroxylamine was lincar in the concentration range of 8.0×10-3~2.0×10-8mol/L with a detection limit of 4.0×10-8mol/L hydroxylamine(3σ).The relative standard deviation(RSD) was 2.0% for 9 repetitive detimes with a good reproducibility and was used to determine hydroxylamine in wastewater.

  2. Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

    Directory of Open Access Journals (Sweden)

    Jiajia Wang

    2013-01-01

    Full Text Available Based on the inhibitory effect of uric acid (UA on luminol-Co2+ chemiluminescence (CL system, a sensitive method for the determination of UA at nanomolar level by flow injection (FI CL was proposed. The proposed method was successfully applied to real-time monitoring of UA excretion in human 24 h urine with different food intake, showing that meats, vegetables, and porridge intake caused differential UA excretions of 879, 798, and 742 mg, respectively. It was also found that UA concentrations in urine under the three kinds of food intake simultaneously reached maximum at 2 h after meals with the values of 417, 318, and 288 μg mL−1, respectively. The UA concentration in human serum was also determined by this approach, and the possible mechanism of luminol-Co2+-UA CL reaction was discussed in detail.

  3. A New Immunoassay Method by Capillary Electrophoresis with Enhanced Chemiluminescence Detection

    Institute of Scientific and Technical Information of China (English)

    Jiao Ning WANG; Ji Cun REN

    2005-01-01

    This paper described a new immunoassay method by capillary electrophoresis with enhanced chemiluminescence (CL) detection system based on luminol-hydrogen peroxide reaction catalyzed by horseradish peroxides (HRP). Using para-iodophenol as a CL enhancer, the detection limit of about 1×10-12 mol/L for HRP was achieved, which corresponded to 1.32×10-5U/mL. In optimal conditions, the free HRP-labeled CA125 antibody (Ab*) and the bound enzyme-labeled complex (Ab*-Ag) were well separated by capillary electrophoresis within 4 min.The assay was successfully used to determine the contents of CA125 in human sera, which were associated with ovarian cancer, and the recoveries of the standard addition experiments were 96 to109 %.

  4. Hybrid algorithm for three-dimensional flame chemiluminescence tomography based on imaging overexposure compensation.

    Science.gov (United States)

    Jin, Ying; Song, Yang; Qu, Xiangju; Li, Zhenhua; Ji, Yunjing; He, Anzhi

    2016-08-01

    Flame tomography of chemiluminescence is a necessary combustion diagnostic technique that provides instantaneous 3D information on flame structure and excited species concentrations. During combustion diagnostics, imaging overexposure always causes missing information, which obviously decreases the accuracy in further reconstructions. In order to compensate imaging overexposure, a hybrid algorithm combining weight correction and Tikhonov's regularization is proposed in this paper. The intensity of the overexposure region can be estimated via the accumulation of weight coefficients. Meanwhile, Tikhonov's regularization is utilized to ameliorate the quality of reconstruction. The numerical simulation quantitatively evaluates the performance of the hybrid algorithm. Additionally, an experiment system consisting of 12 cameras was established to reconstruct the 3D combustion structure of axisymmetric flame with different exposure time settings. This work further investigates dynamic nonaxisymmetric propane diffusion flame. The obtained results show that the hybrid algorithm can effectively reveal the flame structure less influenced by imaging overexposure and achieve better results. PMID:27505371

  5. Potassium permanganate-glutaraldehyde chemiluminescence system catalyzed by gold nanoprisms toward selective determination of fluoride.

    Science.gov (United States)

    Abolhasani, Jafar; Hassanzadeh, Javad; Ghorbani-Kalhor, Ebrahim

    2016-02-01

    Gold and silver nanoparticles (NPs) are shown to exert a positive effect on the chemiluminescence (CL) reaction of permanganate aldehydes. Interestingly, between various shapes examined, Au nanoprisms have the highest beneficial effect. This effect is even more notable in the presence of sodium dodecyl sulfate (SDS) surfactant. UV-vis spectra and transmission electron microscopy were used to characterize the NP shapes and sizes. Furthermore, it was observed that iron(III) ions can slightly increase CL emission of this system. This intensification is very effective in the presence of fluoride ions (F(-)). These observations form the basis of the method for the high sensitive determination of F(-) in the 6-1200 nmol L(-1) concentration range, with a detection limit of 2.1 nmol L(-1). The proposed method has good precision and was satisfactorily used in the selective determination of low concentrations of fluoride ion in real samples. PMID:26110514

  6. Determination of cinnamic acid in human urine by flow injection chemiluminescence

    Directory of Open Access Journals (Sweden)

    Xuemei Fan

    2011-01-01

    Full Text Available It was found that cinnamic acid can react with potassium permanganate in the acidic medium and produce chemiluminescence, which was greatly enhanced by glyoxal. Under the optimum conditions, the linear range for the determination of cinnamic acid was 1.0×10-8 to 1.0×10-4 mol L-1 with a detection limit of 8.0×10-9 mol L-1, the relative standard deviation was 1.7% for 2.0×10-6 mol L-1 cinnamic acid solution in nine repeated measurements. This method was found to be novel0simple0fast and sensitive, it was successfully applied to the determination of cinnamic acid in human urine. Furthermore, the possible reaction mechanism was also discussed.

  7. Design a New Strategy Based on Nanoparticle-Enhanced Chemiluminescence Sensor Array for Biothiols Discrimination

    Science.gov (United States)

    Shahrajabian, Maryam; Hormozi-Nezhad, M. Reza

    2016-01-01

    Array-based sensor is an interesting approach that suggests an alternative to expensive analytical methods. In this work, we introduce a novel, simple, and sensitive nanoparticle-based chemiluminescence (CL) sensor array for discrimination of biothiols (e.g., cysteine, glutathione and glutathione disulfide). The proposed CL sensor array is based on the CL efficiencies of four types of enhanced nanoparticle-based CL systems. The intensity of CL was altered to varying degrees upon interaction with biothiols, producing unique CL response patterns. These distinct CL response patterns were collected as “fingerprints” and were then identified through chemometric methods, including linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA). The developed array was able to successfully differentiate between cysteine, glutathione and glutathione disulfide in a wide concentration range. Moreover, it was applied to distinguish among the above analytes in human plasma. PMID:27574247

  8. Stimulus-response mesoporous silica nanoparticle-based chemiluminescence biosensor for cocaine determination.

    Science.gov (United States)

    Chen, Zhonghui; Tan, Yue; Xu, Kefeng; Zhang, Lan; Qiu, Bin; Guo, Longhua; Lin, Zhenyu; Chen, Guonan

    2016-01-15

    Mesoporous silica nanoparticles (MSN) based controlled release system had been coupled with diverse detection technologies to establish biosensors for different targets. Chemiluminescence (CL) system of luminol/H2O2 owns the characters of simplicity, low cost and high sensitivity, but the targets of which are mostly focused on some oxidants or which can participate in a chemical reaction that yields a product with a role in the CL reaction. In this study, chemiluminescent detection technique had been coupled with mesoporous silica-based controlled released system for the first time to develop a sensitive biosensor for the target which does not cause effect to the CL system itself. Cocaine had been chosen a model target, the MSN support was firstly loaded with glucose, then the positively charged MSN interacted with negatively charged oligonucleotides (the aptamer cocaine) to close the mesopores of MSN. At the present of target, cocaine binds with its aptamer with high affinity; the flexible linear aptamer structured will become stems structured through currently well-defined non-Waston-Crick interactions and causes the releasing of entrapped glucose into the solution. With the assistant of glucose oxidase (GOx), the released glucose can react with the dissolved oxgen to produce gluconic acid and H2O2, the latter can enhance the CL of luminol in the NaOH solution. The enhanced CL intensity has a relationship with the cocaine concentration in the range of 5.0-60μM with the detection limit of 1.43μM. The proposed method had been successfully applied to detect cocaine in serum samples with high selectivity. The same strategy can be applied to develop biosensors for different targets. PMID:26278045

  9. Evaluation of nitrogen dioxide chemiluminescence monitors in a polluted urban environment

    Directory of Open Access Journals (Sweden)

    E. J. Dunlea

    2007-01-01

    Full Text Available Data from a recent field campaign in Mexico City are used to evaluate the performance of the EPA Federal Reference Method for monitoring ambient concentrations of NO2. Measurements of NO2 from standard chemiluminescence monitors equipped with molybdenum oxide converters are compared with those from Tunable Infrared Laser Differential Absorption Spectroscopy (TILDAS and Differential Optical Absorption Spectroscopy (DOAS instruments. A significant interference in the chemiluminescence measurement is shown to account for up to 50% of ambient NO2 concentration during afternoon hours. As expected, this interference correlates well with non-NOx reactive nitrogen species (NOz as well as with ambient O3 concentrations, indicating a photochemical source for the interfering species. A combination of ambient gas phase nitric acid and alkyl and multifunctional alkyl nitrates is deduced to be the primary cause of the interference. Observations at four locations at varying proximities to emission sources indicate that the percentage contribution of HNO3 to the interference decreases with time as the air parcel ages. Alkyl and multifunctional alkyl nitrate concentrations are calculated to be reach concentrations as high as several ppb inside the city, on par with the highest values previously observed in other urban locations. Averaged over the MCMA-2003 field campaign, the CL NOx monitor interference resulted in an average measured NO2 concentration up to 22% greater than that from co-located spectroscopic measurements. Thus, this interference has the potential to initiate regulatory action in areas that are close to non-attainment and may mislead atmospheric photochemical models used to assess control strategies for photochemical oxidants.

  10. Highly sensitive multianalyte immunochromatographic test strip for rapid chemiluminescent detection of ractopamine and salbutamol

    International Nuclear Information System (INIS)

    Graphical abstract: A multianalyte immunochromatographic test strip was developed for the rapid detection of two β2-agonists. Due to the application of chemiluminescent detection, this quantitative method shows much higher sensitivity. - Highlights: • An immunochromatographic test strip was developed for detection of multiple β2-agonists. • The whole assay process can be completed within 20 min. • The proposed method shows much higher sensitivity due to the application of CL detection. • It is a portable analytical tool suitable for field analysis and rapid screening. - Abstract: A novel immunochromatographic assay (ICA) was proposed for rapid and multiple assay of β2-agonists, by utilizing ractopamine (RAC) and salbutamol (SAL) as the models. Owing to the introduction of chemiluminescent (CL) approach, the proposed protocol shows much higher sensitivity. In this work, the described ICA was based on a competitive format, and horseradish peroxidase-tagged antibodies were used as highly sensitive CL probes. Quantitative analysis of β2-agonists was achieved by recording the CL signals of the probes captured on the two test zones of the nitrocellulose membrane. Under the optimum conditions, RAC and SAL could be detected within the linear ranges of 0.50–40 and 0.10–50 ng mL−1, with the detection limits of 0.20 and 0.040 ng mL−1 (S/N = 3), respectively. The whole process for multianalyte immunoassay of RAC and SAL can be completed within 20 min. Furthermore, the test strip was validated with spiked swine urine samples and the results showed that this method was reliable in measuring β2-agonists in swine urine. This CL-based multianalyte test strip shows a series of advantages such as high sensitivity, ideal selectivity, simple manipulation, high assay efficiency and low cost. Thus, it opens up new pathway for rapid screening and field analysis, and shows a promising prospect in food safety

  11. Stimulus-response mesoporous silica nanoparticle-based chemiluminescence biosensor for cocaine determination.

    Science.gov (United States)

    Chen, Zhonghui; Tan, Yue; Xu, Kefeng; Zhang, Lan; Qiu, Bin; Guo, Longhua; Lin, Zhenyu; Chen, Guonan

    2016-01-15

    Mesoporous silica nanoparticles (MSN) based controlled release system had been coupled with diverse detection technologies to establish biosensors for different targets. Chemiluminescence (CL) system of luminol/H2O2 owns the characters of simplicity, low cost and high sensitivity, but the targets of which are mostly focused on some oxidants or which can participate in a chemical reaction that yields a product with a role in the CL reaction. In this study, chemiluminescent detection technique had been coupled with mesoporous silica-based controlled released system for the first time to develop a sensitive biosensor for the target which does not cause effect to the CL system itself. Cocaine had been chosen a model target, the MSN support was firstly loaded with glucose, then the positively charged MSN interacted with negatively charged oligonucleotides (the aptamer cocaine) to close the mesopores of MSN. At the present of target, cocaine binds with its aptamer with high affinity; the flexible linear aptamer structured will become stems structured through currently well-defined non-Waston-Crick interactions and causes the releasing of entrapped glucose into the solution. With the assistant of glucose oxidase (GOx), the released glucose can react with the dissolved oxgen to produce gluconic acid and H2O2, the latter can enhance the CL of luminol in the NaOH solution. The enhanced CL intensity has a relationship with the cocaine concentration in the range of 5.0-60μM with the detection limit of 1.43μM. The proposed method had been successfully applied to detect cocaine in serum samples with high selectivity. The same strategy can be applied to develop biosensors for different targets.

  12. Chemiluminescence-based multivariate sensing of local equivalence ratios in premixed atmospheric methane-air flames

    Energy Technology Data Exchange (ETDEWEB)

    Tripathi, Markandey M.; Krishnan, Sundar R.; Srinivasan, Kalyan K.; Yueh, Fang-Yu; Singh, Jagdish P.

    2011-09-07

    Chemiluminescence emissions from OH*, CH*, C2, and CO2 formed within the reaction zone of premixed flames depend upon the fuel-air equivalence ratio in the burning mixture. In the present paper, a new partial least square regression (PLS-R) based multivariate sensing methodology is investigated and compared with an OH*/CH* intensity ratio-based calibration model for sensing equivalence ratio in atmospheric methane-air premixed flames. Five replications of spectral data at nine different equivalence ratios ranging from 0.73 to 1.48 were used in the calibration of both models. During model development, the PLS-R model was initially validated with the calibration data set using the leave-one-out cross validation technique. Since the PLS-R model used the entire raw spectral intensities, it did not need the nonlinear background subtraction of CO2 emission that is required for typical OH*/CH* intensity ratio calibrations. An unbiased spectral data set (not used in the PLS-R model development), for 28 different equivalence ratio conditions ranging from 0.71 to 1.67, was used to predict equivalence ratios using the PLS-R and the intensity ratio calibration models. It was found that the equivalence ratios predicted with the PLS-R based multivariate calibration model matched the experimentally measured equivalence ratios within 7%; whereas, the OH*/CH* intensity ratio calibration grossly underpredicted equivalence ratios in comparison to measured equivalence ratios, especially under rich conditions ( > 1.2). The practical implications of the chemiluminescence-based multivariate equivalence ratio sensing methodology are also discussed.

  13. Chemiluminescence behavior of the carbon dots and the reduced state carbon dots

    Energy Technology Data Exchange (ETDEWEB)

    Teng, Ping [Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715 (China); Xie, Jianxin [College of Resources and Environment, Yuxi Normal University, Yuxi, Yunnan 653100 (China); Long, Yijuan; Huang, Xiaoxiao; Zhu, Rui; Wang, Xiliang; Liang, Liping [Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715 (China); Huang, Yuming, E-mail: ymhuang@swu.edu.cn [Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715 (China); Zheng, Huzhi, E-mail: zhenghz@swu.edu.cn [Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715 (China)

    2014-02-15

    Potassium permanganate (KMnO{sub 4}) can react with two different carbon nanoparticles, i.e., carbon dots (CDs) and reduced state carbon dots (r-CDs), in a strong acid medium to generate chemiluminescence (CL). Furthermore, the different CL intensities and CL behaviors due to the different surface groups on these two kinds of carbon nanoparticles were confirmed. CL spectra, fluorescence spectra, UV–vis absorption spectra, and electron paramagnanetic resonance spectra were applied to investigate the CL mechanism. The main reaction pathways were proposed as follows: for the CL reaction between CDs and KMnO{sub 4}, the excited states of CDs (CDs{sup ⁎}) and Mn(II) (Mn(II){sup ⁎}) emerged as KMnO{sub 4} could inject holes into CDs, then, the CDs{sup ⁎} and Mn(II){sup ⁎} acted as luminophors to yield CL; in the r-CDs-KMnO{sub 4} system, r-CDs were oxidized by KMnO{sub 4} directly, and CDs{sup ⁎} and Mn(II){sup ⁎} were produced, at the same time, CL occurred. What is more interesting is that the CL intensity of the r-CD system is stronger than that of the CD system, which confirms that functional groups have strong effect on the CL behavior. It inspired us that new carbon nanoparticles with excellent luminous performance can be designed by tuning their surface groups. -- Highlights: • Carbon dots (CDs) and reduced state carbon dots (r-CDs) can react with potassium permanganate (KMnO{sub 4}) in a strong acid to generate chemiluminescence (CL). • With different surface groups, the CL intensity of r-CDs-KMnO{sub 4} system is different from that of CDs-KMnO{sub 4} system. • The CL mechanisms of the two systems were investigated.

  14. Rapid determination of Papaver somniferum alkaloids in process streams using monolithic column high-performance liquid chromatography with chemiluminescence detection

    International Nuclear Information System (INIS)

    We have combined high-performance liquid chromatography (HPLC) separations using a monolithic column with acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection in a rapid and highly sensitive method to monitor the process of extracting opiate alkaloids from Papaver somniferum. Due to the high flow rates allowed with the monolithic column and the inherent selectivity of the chemiluminescence reactions, the four predominant alkaloids - morphine, codeine, oripavine and thebaine - were determined in less than 2 min. The results obtained with numerous process samples compared favourable with those of the standard HPLC methodology. Limits of detection were 1 x 10-10 M, 5 x 10-10 M, 5 x 10-10 M and 1 x 10-9 M, for morphine, codeine, oripavine and thebaine, respectively

  15. Measurements of essential oil extract and antioxidant in Syrian Myrtus communis L. leaves using photo chemiluminescence assay

    International Nuclear Information System (INIS)

    The essential oil extracts and antioxidant measurements of Syrian Myrtus communis L. leaves as hydrophilic and hydrophobic existence species have been carried out. The plant leaves as a source of antioxidants was tested by the influence of its aqueous and essential oil extracts on the yield of photo chemiluminescence, PCL solution applying very sensitive and reliable method. By means of a photo chemiluminescence assay, it was possible to assess the total antioxidants capacity of hydrophilic and hydrophobic species existence in Syrian Myrtus communis L. leaves. It has been found that, the integral antioxidant capacity measurements value of Syrian Myrtus communis L. leaves was found in Kurdaha site which has a value of 465.67 1.18 nmol TE/g DM (total Trolox equivalent /gram of Dry material) . The following three mainly chemical species were found in the essential oil extracts: -Pinene, Cineole and Limonene. (author)

  16. [Determination of antioxidation of the extract from Chinese medicine Morinda officinalis How by flow injection chemiluminescence and spectroscopy].

    Science.gov (United States)

    Wu, Yong-Jun; Shi, Jie; Qu, Ling-Bo; Li, Fei-Fei; Li, Xiao-Jing; Wu, Yi-Ming

    2006-09-01

    Flow injection chemiluminescence analysis was used to determine the antioxidation activity of extract from Morinda officinalis How. The determination was based on the inhibition effect of Morinda officinalis How extraction in the chemiluminescence reaction of luminol - H2O2-CuSO4 system, and vitamin C was used as positive control. It was showed that Morinda officinalis How original liquid has obvious anti-radical activity. Spectroscopy was applied to determine the inhibition ratio of Morinda officinalis How extraction for the superoxide anion and hydroxy radicals in the special system. It was shown that the extraction from Morinda officinalis How could obviously scavenge superoxide anion and hydroxy radicals. It is suggested that the Chinese Medicine Morinda Officinalis How is a potential antioxidation activity medicine. PMID:17112048

  17. A Capillary Electrophoresis Detection Scheme for Water-soluble Vitamins Based on Luminol - BrO- Chemiluminescence System

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    A novel chemiluminescence detection scheme has been developed for detecting water-soluble vitamins following capillary electrophoresis (CE) separation. This detection was based on the inhibitory effect of vitamins on the CL reaction between luminol and BrO- in basic aqueous solution. Detection of vitamins was accomplished with a borate-based background electrolyte at pH 9.2. The luminol was used as a component of the separation carrier electrolyte.

  18. Partial least squares and principal components analysis of wine vintage by high performance liquid chromatography with chemiluminescence detection.

    Science.gov (United States)

    Bellomarino, S A; Parker, R M; Conlan, X A; Barnett, N W; Adams, M J

    2010-09-23

    HPLC with acidic potassium permanganate chemiluminescence detection was employed to analyse 17 Cabernet Sauvignon wines across a range of vintages (1971-2003). Partial least squares regression analysis and principal components analysis was used in order to investigate the relationship between wine composition and vintage. Tartaric acid, vanillic acid, catechin, sinapic acid, ethyl gallate, myricetin, procyanadin B and resveratrol were found to be important components in terms of differences between the vintages.

  19. Study on Enhancement Principle and Stabilization for the Luminol-H2O2-HRP Chemiluminescence System.

    Directory of Open Access Journals (Sweden)

    Lihua Yang

    Full Text Available A luminol-H2O2-HRP chemiluminescence system with high relative luminescent intensity (RLU and long stabilization time was investigated. First, the comparative study on the enhancement effect of ten compounds as enhancers to the luminol-H2O2-HRP chemiluminescence system was carried out, and the results showed that 4-(imidazol-1-ylphenol (4-IMP, 4-iodophenol (4-IOP, 4-bromophenol (4-BOP and 4-hydroxy-4'-iodobiphenyl (HIOP had the best performance. Based on the experiment, the four enhancers were dissolved in acetone, acetonitrile, methanol, and dimethylformamide (DMF with various concentrations, the results indicated that 4-IMP, 4-IOP, 4-BOP and HIOP dissolved in DMF with the concentrations of 0.2%, 3.2%, 1.6% and 3.2% could get the highest RLU values. Subsequently, the influences of pH, ionic strength, HRP, 4-IMP, 4-IOP, 4-BOP, HIOP, H2O2 and luminol on the stabilization of the luminol-H2O2-HRP chemiluminescence system were studied, and we found that pH value, ionic strength, 4-IMP, 4-IOP, 4-BOP, HIOP, H2O2 and luminol have little influence on luminescent stabilization, while HRP has a great influence. In different ranges of HRP concentration, different enhancers should be selected. When the concentration is within the range of 0~6 ng/mL, 4-IMP should be selected. When the concentration of HRP ranges from 6 to 25 ng/mL, 4-IOP was the best choice. And when the concentration is within the range of 25~80 ng/mL, HIOP should be selected as the enhancer. Finally, the three well-performing chemiluminescent enhanced solutions (CESs have been further optimized according to the three enhancers (4-IMP, 4-IOP and HIOP in their utilized HRP concentration ranges.

  20. Indirect Flow Injection Chemiluminescence Method for the Determination of Tetracyclines Using Cu(Ⅱ) as a Probe Ion

    Institute of Scientific and Technical Information of China (English)

    Su Qin HAN; Er Bao LIU; Hua LI

    2005-01-01

    This paper reported an indirect flow injection chemiluminescence (FI-CL) method for the determination of the drugs tetracycline (TC), chlortetracycline (CTC) and oxytetracycline (OTC) using Cu(Ⅱ) as a probe ion. The CL reaction was induced on-line and after injection of the sample the negative peaks appeared as a result of complexation. The method was applied to the determination of TCs in pharmaceuticals and human urine with recoveries in the range 95~105%.

  1. Evaluation of LIAISON Treponema Screen, a Novel Recombinant Antigen-Based Chemiluminescence Immunoassay for Laboratory Diagnosis of Syphilis

    OpenAIRE

    Marangoni, Antonella; Sambri, Vittorio; Accardo, Silvia; Cavrini, Francesca; D'Antuono, Antonietta; Moroni, Alessandra; Storni, Elisa; Cevenini, Roberto

    2005-01-01

    The purpose of this study was to evaluate the diagnostic performance of LIAISON Treponema Screen (DiaSorin, Saluggia, Italy), a new automated chemiluminescence immunoassay (CLIA), in comparison with that of rapid plasma reagin (RPR) and the following currently used treponemal tests: hemagglutination test (TPHA), immunoenzymatic assay (EIA), and Western blot (WB). First, a retrospective study was performed with a panel of 2,494 blood donor sera, a panel of 131 clinical and serologically charac...

  2. Effect of the dimetilsulfoxido in the response chemiluminescent and the consumption of oxygen of neutrophils activated human

    International Nuclear Information System (INIS)

    Dimethylsulfoxide (DMSO), a hydroxyl radical scavenger, exerted a dose dependent inhibition on the luminol and lucigenin-enhanced chemiluminescent responses of human neutrophils activated with soluble and particulate stimulants. DMSO inhibition of the luminol chemiluminescense induced by calcium ionophore A23187 was probably due to OH scavenging, whereas inhibition of the lucigenin chemiluminescence suggested DMSO negatively affects the NADPH-dependent membrane oxidase of neutrophils. In agreement with this, DMSO moderately inhibited O2 consumption in PMN suspensions stimulated with chemotactic peptide and opsonized zymosan-induced luminol chemiluminescense was observed only when added before or in conjunction with stimulants, whereas A23187-induced chemiluminescense was inhibited by DMSO regardless of time of addition. Washing of DMSO-treated PMN resulted in increased luminol enhanced chemiluminescense in response to chemotactic peptide and opsonized zymosan. This is consistent with the idea that DMSO may be interfering with activation of the membrane subunits of the oxidase by translocation and docking of the cytoplasmic, regulatory subunits. These data imply that DMSO inhibits neutrophil chemiluminescense both by OH scavenging and interfering with oxidase activation. Key words:Dimethylsulfoxide, chemiluminescent, luminol, lucigenin,neutrophils

  3. Inhibition of chemiluminescence and chemotactic activity of phagocytes in vitro by the extracts of selected medicinal plants.

    Science.gov (United States)

    Jantan, Ibrahim; Harun, Nurul Hikmah; Septama, Abdi Wira; Murad, Shahnaz; Mesaik, M A

    2011-04-01

    The methanol extracts of 20 selected medicinal plants were investigated for their effects on the respiratory burst of human whole blood, isolated human polymorphonuclear leukocytes (PMNs) and isolated mice macrophages using a luminol/lucigenin-based chemiluminescence assay. We also tested the effect of the extracts on chemotactic migration of PMNs using the Boyden chamber technique. The extracts of Curcuma domestica L., Phyllanthus amarus Schum & Thonn and C. xanthorrhiza Roxb. were the samples producing the strongest oxidative burst of PMNs with luminol-based chemiluminescence, with IC(50) values ranging from 0.5 to 0.7 μg/ml. For macrophage cells, the extracts which showed strong suppressive activity for luminol-based chemiluminescence were C. xanthorrhiza and Garcinia mangostana L. Among the extracts studied, C. mangga Valton & Vazsjip, Piper nigrum L. and Labisia pumila var. alata showed strong inhibitory activity on lucigenin-amplified oxidative burst of PMNs, with IC(50) values ranging from 0.9 to 1.5 μg/ml. The extracts of Zingiber officinale Rosc., Alpinia galangal (L.) Willd and Averrhoa bilimbi Linn showed strong inhibition on the chemotaxic migration of cells, with IC(50) values comparable to that of ibuprofen (1.5 μg/ml). The results suggest that some of these plants were able to modulate the innate immune response of phagocytes at different steps, emphasizing their potential as a source of new immunomodulatory agents. PMID:21184195

  4. High-performance liquid chromatographic assay of parabens in wash-off cosmetic products and foods using chemiluminescence detection

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Qunlin [Department of Chemistry, University of Science and Technology of China, Jinzai Road 96, Hefei, Anhui 230026 (China); Lian Mei [Department of Chemistry, University of Science and Technology of China, Jinzai Road 96, Hefei, Anhui 230026 (China); Liu Lijuan [Department of Chemistry, University of Science and Technology of China, Jinzai Road 96, Hefei, Anhui 230026 (China); Cui Hua [Department of Chemistry, University of Science and Technology of China, Jinzai Road 96, Hefei, Anhui 230026 (China)]. E-mail: hcui@ustc.edu.cn

    2005-04-29

    A new method for the simultaneous determination of parabens including methylparaben, ethylparaben, propylparaben, and butylparaben by high-performance liquid chromatography (HPLC) coupled with chemiluminescence detection was developed. The procedure was based on the chemiluminescent enhancement by parabens of the cerium(IV)-rhodamine 6G system in the strong sulfuric acid medium. The good separation of parabens was carried out with an isocratic elution using a mixture of methanol and water (60:40, v/v) within 8.5 min. Under the optimized conditions, a linear working range extends three orders of magnitude with the relative standard deviations of intra- and inter-day precision below 4.5%, and the detection limits were 1.9 x 10{sup -9}, 2.7 x 10{sup -9}, 3.9 x 10{sup -9}, and 5.3 x 10{sup -9} g ml{sup -1} for methylparaben, ethylparaben, propylparaben, and butylparaben, respectively. The chemiluminescence reaction was well compatible with the mobile phase of high-performance liquid chromatography. The proposed method has been successfully applied to the assay of parabens in wash-off cosmetic products and foods with the minimal sample preparation.

  5. A multiplex chemiluminescent biosensor for type B-fumonisins and aflatoxin B1 quantitative detection in maize flour.

    Science.gov (United States)

    Zangheri, Martina; Di Nardo, Fabio; Anfossi, Laura; Giovannoli, Cristina; Baggiani, Claudio; Roda, Aldo; Mirasoli, Mara

    2015-01-01

    A multiplex chemiluminescent biosensor for simple, rapid and ultrasensitive on-site quantification of aflatoxin B1 and type B-fumonisins in maize samples has been developed. The biosensor integrates a multiplex indirect competitive lateral flow immunoassay (LFIA) based on enzyme-catalyzed chemiluminescence detection and a highly sensitive portable charge-coupled device (CCD) camera, employed in a lensless "contact" imaging configuration. The developed assay requires a simple extraction of the analytes from maize flour samples followed by their detection with a 30 min assay time. The use of chemiluminescence detection allowed accurate and objective analytes quantification, enabling simultaneous detection of type B-fumonisins and aflatoxin B1 down to 6 μg kg(-1) and 1.5 μg kg(-1), respectively, thus fulfilling the standards imposed by the legislation of European Union. Maize flour samples spiked with both analytes were subjected to multiplex analysis obtaining recoveries ranging from 80 to 115% and the coefficient of variation below 20%. Finally, analysis of naturally contaminated maize samples resulted in a good agreement between CL-LFIA and a validated confirmatory HPLC-UV and commercial ELISA kit, obtaining recoveries in the range 88-120%. The proposed CL-LFIA protocol is rapid, inexpensive, easy-to-use, and fit for the purpose of rapid screening of mycotoxins in maize flour. PMID:25374970

  6. Menadione-catalyzed luminol chemiluminescence assay for the rapid detection of viable bacteria in foods under aerobic conditions.

    Science.gov (United States)

    Kawasaki, S; Yamashoji, S; Asakawa, A; Isshiki, K; Kawamoto, S

    2004-12-01

    A menadione-catalyzed luminol chemiluminescence assay was developed for the rapid detection and estimation of viable bacteria in foods. The principle of this assay is based on the extracellular menadione-catalyzed active oxygen spieces (O2- and H2O2) generated by the activity of NAD(P)H:menadione oxidoreductase in viable cells. This luminol chemiluminescence assay requires 10 min for the incubation of cells with menadione and then 2 s for the measurement of chemiluminescence intensity after an injection of luminol solution without the treatment of cell lysis. This method was evaluated using liquid food samples of milk, vegetable juice, green tea, and coffee spiked with Escherichia coli ATCC 25922. The study result revealed that E. coli contamination at 1 to 10 CFU/ml in these foods could be detected after incubation at 37 degrees C for 7 h in an enrichment medium; however, the green tea and coffee samples requires filtration. This method could be a useful tool for the rapid evaluation of microbial food contamination. PMID:15633684

  7. High-performance liquid chromatographic assay of parabens in wash-off cosmetic products and foods using chemiluminescence detection

    International Nuclear Information System (INIS)

    A new method for the simultaneous determination of parabens including methylparaben, ethylparaben, propylparaben, and butylparaben by high-performance liquid chromatography (HPLC) coupled with chemiluminescence detection was developed. The procedure was based on the chemiluminescent enhancement by parabens of the cerium(IV)-rhodamine 6G system in the strong sulfuric acid medium. The good separation of parabens was carried out with an isocratic elution using a mixture of methanol and water (60:40, v/v) within 8.5 min. Under the optimized conditions, a linear working range extends three orders of magnitude with the relative standard deviations of intra- and inter-day precision below 4.5%, and the detection limits were 1.9 x 10-9, 2.7 x 10-9, 3.9 x 10-9, and 5.3 x 10-9 g ml-1 for methylparaben, ethylparaben, propylparaben, and butylparaben, respectively. The chemiluminescence reaction was well compatible with the mobile phase of high-performance liquid chromatography. The proposed method has been successfully applied to the assay of parabens in wash-off cosmetic products and foods with the minimal sample preparation

  8. Carbon nanostructures as catalytic support for chemiluminescence of sulfur compounds in a molecular emission cavity analysis system.

    Science.gov (United States)

    Safavi, Afsaneh; Maleki, Norouz; Doroodmand, Mohammad Mahdi; Koleini, Mohammad Mehdi

    2009-06-30

    The effect of different substrates including stainless steel, activated carbon, single-walled carbon nanotubes (SWCNTs), multi-walled carbon nanotubes (MWCNTs), fullerenes (C60, C70, etc.) and SWCNTs doped with iron and palladium nanoparticles were compared for catalytic chemiluminescence reaction of sulfur compounds in a flame-containing cavity of molecular emission cavity analysis (MECA) system. Different forms of CNT substrates were fabricated using electric arc-discharge method. The blue emission of excited S2 was monitored using a CCD camera. The results demonstrate that, due to the high surface area, plenty of basal planes, high thermal conductivity, and high flexibility of the carbon nanostructure as appropriate support, carbon nanostructures play an important role in catalytic chemiluminescence emission of sulfur compounds in MECA. Moreover, the presence of metallic nanoparticles doped on carbon nanostructures enhances their catalytic effect. The results revealed that under similar conditions, SWCNTs/Pd doped nanoparticles, SWCNTs/Fe doped nanoparticles, SWCNTs, MWCNTs and fullerenes have the most catalytic effects on chemiluminescence of sulfur compounds, respectively. PMID:19463563

  9. Laminated paper-based analytical devices (LPAD) with origami-enabled chemiluminescence immunoassay for cotinine detection in mouse serum.

    Science.gov (United States)

    Liu, Wei; Cassano, Christopher L; Xu, Xin; Fan, Z Hugh

    2013-11-01

    Laminated paper-based analytical devices (LPAD) with origami-enabled chemiluminescence immunoassay have been developed for the detection of cotinine, a secondhand smoke (SHS) biomarker. The devices were fabricated by a craft-cutter to define flow channels, followed by lamination. This approach of cutting/lamination to fabricate LPAD is very similar to making an identification card, offering advantages in simplicity and rugged backing when compared to the common method of patterning paper using SU-8 or wax. We also developed a protocol of localized incision and paper-folding to isolate the detection zone from flow channels; the simple origami step eliminated possible reagent diffusion and flow during antibody immobilization steps and numerous washings. By incorporating luminol-based chemiluminescence for detecting horseradish peroxidase-conjugated cotinine, we employed origami-enabled LPAD to detect cotinine in mouse serum using competitive immunoassay. The detection limit was determined to be 5 ng/mL, a clinically relevant concentration. We believe that LPAD with chemiluminescence detection provides a new platform of low cost and sensitive assays for cotinine detection.

  10. Chemiluminescence determination of didanosine%化学发光法测定去羟肌苷

    Institute of Scientific and Technical Information of China (English)

    李建军; 郑伟; 屈凌波; 杨冉

    2009-01-01

    To develop a method for the determination of didanosine by chemiluminescence (CL). Didanosine can greatly inhibit the intensity of the chemiluminescence reaction between KMnO4 and sulphite in a nitric acid medium, and there was a good linear relationship between the inhibition and the concentration of didanosine. Based on this phenomenon, didanosine was determined. The linear range of didanosine was 4.0×10~(-7)~1.0×10~(-4) g/mL and the limit of detection was 9.6×10~(-8) g/mL. The relative standard deviation (re=11) was 2.0% for 8.0×10~(-5) g/mL didanosine. The method could be applied to the determination of didanosine in capsule due to its simplicity, high efficiency and high sensitivity.%建立了快速检测去羟肌苷的化学发光方法. 在HNO3介质中, 去羟肌苷对KMnO_4-Na_2SO_3化学发光体系有较强的抑制作用, 且抑制的程度与去羟肌苷的质量浓度在一定范围内呈良好的线性关系, 据此可以测定去羟肌苷含量. 方法的线性范围为4.0×10~(-7)~1.0×10~(-4) g/mL, 检出限为9.6×10~(-8) g/mL. 对8.0×10~(-5) g/mL去羟肌苷测定的相对标准偏差为2.0% (n=11). 方法适用于胶囊中去羟肌苷的含量测定.

  11. Highly sensitive multianalyte immunochromatographic test strip for rapid chemiluminescent detection of ractopamine and salbutamol

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Hongfei; Han, Jing; Yang, Shijia; Wang, Zhenxing; Wang, Lin; Fu, Zhifeng, E-mail: fuzf@swu.edu.cn

    2014-08-11

    Graphical abstract: A multianalyte immunochromatographic test strip was developed for the rapid detection of two β{sub 2}-agonists. Due to the application of chemiluminescent detection, this quantitative method shows much higher sensitivity. - Highlights: • An immunochromatographic test strip was developed for detection of multiple β{sub 2}-agonists. • The whole assay process can be completed within 20 min. • The proposed method shows much higher sensitivity due to the application of CL detection. • It is a portable analytical tool suitable for field analysis and rapid screening. - Abstract: A novel immunochromatographic assay (ICA) was proposed for rapid and multiple assay of β{sub 2}-agonists, by utilizing ractopamine (RAC) and salbutamol (SAL) as the models. Owing to the introduction of chemiluminescent (CL) approach, the proposed protocol shows much higher sensitivity. In this work, the described ICA was based on a competitive format, and horseradish peroxidase-tagged antibodies were used as highly sensitive CL probes. Quantitative analysis of β{sub 2}-agonists was achieved by recording the CL signals of the probes captured on the two test zones of the nitrocellulose membrane. Under the optimum conditions, RAC and SAL could be detected within the linear ranges of 0.50–40 and 0.10–50 ng mL{sup −1}, with the detection limits of 0.20 and 0.040 ng mL{sup −1} (S/N = 3), respectively. The whole process for multianalyte immunoassay of RAC and SAL can be completed within 20 min. Furthermore, the test strip was validated with spiked swine urine samples and the results showed that this method was reliable in measuring β{sub 2}-agonists in swine urine. This CL-based multianalyte test strip shows a series of advantages such as high sensitivity, ideal selectivity, simple manipulation, high assay efficiency and low cost. Thus, it opens up new pathway for rapid screening and field analysis, and shows a promising prospect in food safety.

  12. Chemiluminescence accompanied by the reaction of acridinium ester and manganese (II).

    Science.gov (United States)

    Ren, Lingling; Cui, Hua

    2014-11-01

    An acridinium ester (AE) alkaline solution can react with Mn(II) to generate a strong chemiluminescence (CL) centered at 435 nm. The effects of reaction conditions such as pH and Mn(II) concentration on CL intensity were examined. In order to explore the CL mechanism, the effect of oxygen on the CL reaction was examined and an X-ray photoelectron spectroscopy study of the reaction precipitate was carried out. The results indicated that oxygen participated in the CL reaction and Mn(IV) was the primary product in the system. A possible mechanism was proposed that involved two pathways: (1) dissolved oxygen was reduced to reactive oxygen radicals by Mn(II), these reactive intermediates then reacted with AE to produce excited state acridone; (2) Mn(II) could reduce AE to partly reduced AE, which then reacted with oxygen to form excited state acridone. The reactions of other metal ions with AE were also tested, and only Mn(II) was shown to trigger strong CL emission of AE, which indicated that the system had good selectivity for Mn(II). PMID:24677387

  13. Clinical significance of quantitative analysis of thyroid peroxidase antibody (TPOAb) with chemiluminescence enzyme immunoassay

    International Nuclear Information System (INIS)

    Objective: The only method of laboratory diagnosis for autoimmune thyroid diseases used to be serum TGA and TMA detections. Morerecently, quantitative analysis of TPOAb has been introduced. To assess the relative sensitivity of these tests , positive rates detected with the respective tests were compared. Methods: Serum TGA, TMA (with RIA) and TPOAb (with chemiluminescence enzyme immunoassay) were simultaneously detected in 998 cases of thyroid diseases (hyperthyroidism 307, Hashimoto's disease 193, simple goiter 498). For complementary sake, fine needle aspiration cytology was obtained in a number of cases including all the patients with Hashimoto's disease. Results: Positive detection rate of TPOAb in three groups of patients (hyperthyroidism, Hashimoto's, simple goiter) was 81.76%, 96.89 % and 42.97% respectively. With TMA, the positive rate was only 54.72%, 65.80%, 22.09% respectively. About one third more cases would be detected with the newer method. Conclusion: For the laboratory detection of auto immune thyroid diseases, quantitative analysis of TPOAb is much wore sensitive than the conventional TMA detection. (authors)

  14. A chemiluminescence-based continuous flow aqueous ozone analyzer using photoactivated chromotropic acid.

    Science.gov (United States)

    Takayanagi, Toshio; Dasgupta, Purnendu K

    2005-05-15

    Ozone has become the oxidant of choice for water disinfection, especially in large water treatment facilities. This paper describes a fast and sensitive method for the determination of ozone content by reaction with photoactivated chromotropic acid (CA, 4,5-dihydroxynaphthalene-2,7-disulfonic acid), which results in intense chemiluminescence (CL). Freshly ozonated water from a recirculating ozonizer/reservoir is injected into a carrier stream of deionized water in the flow-injection mode. This flow mixes with a stream of photoactivated CA solution in a spiral cell placed directly on top of an inexpensive miniature (8mm diameter active area) photomultiplier tube (PMT). Alkaline CA is photoactivated by passing it through a FEP-Teflon((R)) coil (residence time approximately 50s) wrapped around a 1W UV lamp emitting at 254nm; without photoactivation, the signal is approximately 70-fold lower. The S/N=3 limit of detection for aqueous ozone is 3mugl(-1) and good response slope is obtained up to an ozone concentration of 1.4mgl(-1), the highest that could be made in this study. The response obeyed a quadratic equation with r(2)=0.9984. No interference from permanganate ion is observed. The proposed system was applied to the monitoring of ozonation status of a playa lake water that exhibited significant ozone demand.

  15. Oscillating chemiluminescence in rhodamine B-induced Belousov-Zhabotinsky reaction catalyzed by Ce(Ⅳ)

    Institute of Scientific and Technical Information of China (English)

    DUAN ChunFeng; LIU GuangMing; ZHANG ZhiFeng; CUI Hua

    2009-01-01

    It was found that rhodamine B could induce oscillating chemiluminescence (OCL) from the Ce4+-cata-lyzed Belousov-Zhabotinsky reaction. This new OCL system, i.e., rhodamine B-malonic acid-bromate-Ce(IV)-sulfuric acid, exhibited two clearly distinguished emission peaks in each oscillation period. The initial concentrations of the reactants strongly influenced the oscillation pattern. For the study of the CL mechanism, a platform for a versatile and simultaneous potential and CL measurement was estab-lished to compare the potential oscillation with the CL oscillation behavior of this system. The CL spectra, UV-visible absorption spectra and time-resolved fluorescence spectra of this OCL system were studied. A possible, simplified mechanism for the OCL is proposed. It is suggested that the generation of the two CL peaks is likely due to the oxidation of the intermediate of rhodamine B by Co(IV) and Br2, respectively. This work provided a new method and platform to research the complex chemical oscilla-tions.

  16. New chemiluminescent substrates of paraoxonase 1 with improved specificity: synthesis and properties.

    Science.gov (United States)

    Abulimite, Zulipiyan; Mu, Xiaojing; Xiao, Shangyou; Liu, Min; Li, Quandan; Chen, Gang

    2015-05-01

    Paraoxonase 1 (PON1) is an important hydrolase, and the enzyme activity decreases in patients with liver disease, diabetes, coronary heart disease, etc. Phenyl acetate and organophosphates are usually employed as substrates for serum PON1 activity assay. However, phenyl acetate for arylesterase activity assay exhibits disadvantage of high background. According to properties of PON1, four new chemiluminescent acridinium esters were designed, prepared through three steps, and characterized with (1)H NMR and mass spectrometry (MS) data, and their properties as PON1 substrates were investigated. The hydrolyses of the four compounds catalyzed by recombinant human PON1 (rhPON1) (or serum) followed first-order kinetics within 22 min. The PON1 activator (NaCl, 0.10 mol L(-1)) could boost the rhPON1-mediated and serum-mediated hydrolyses of the acridinium esters to 2.01 ~ 2.26 folds, but 1.0 mol L(-1) NaCl decreased the serum arylesterase activity. RhPON1 showed selectivity over other serum esterases such as lipase, acetylcholinesterase, and esterase D more than 300 folds. By using ethylene diamine tetraacetic acid (EDTA) inhibitor, the specificities of the four substrates toward serum PON1 were determined as 78.3 ~ 92.9%, which is improved than that of the model compound 9-(4-chloro-phenoxycarbonyl)-10-methylacridinium ester triflate. Due to low toxicity, high specificity, and sensitivity of the substrates, they are useful for serum PON1 activity assay. PMID:25809994

  17. Determination of catecholamines by ion chromatography coupled to acidic potassium permanganate chemiluminescence detection

    Institute of Scientific and Technical Information of China (English)

    Hong Wei Wu; Mei Lan Chen; Dan Shou; Yan Zhu

    2012-01-01

    A simple,fast,sensitive,highly selective and eco-friendly analytical method for the determination of catecholamines in human urine by ion chromatography (IC) with chemiluminescence (CL) detection was described in this paper.Using 12 mmoi/L H2SO4 without any organic additive as eluent,three catecholamines including epinephrine (EP),norepinephrine (NE) and dopamine (DA)were well separated on a cation-exchange column.The CL detection was based on the reaction of analytes with acidic potassium permanganate in the presence of formaldehyde as an enhancer.The absence of methanol and acetonitrile in eluent made the proposed method more sensitive and eco-friendly.Under the optimal conditions,the linear range of the proposed method was in the range of 0.02-0.5 μg/mL.The limit of detection (LOD) was in the range of 0.6 and 5.1 μg/L.The relative standard deviations (RSD) for 0.1 μg/mL mixed standard solution were in the range of 0.8-1.9% (n =11).The method has been applied to the determination of catecholamines in human urine successfully.Excellent spiked recoveries were achieved for catecholamines ranged from 91.2% to 112.7%.

  18. Influence of quantum dot's quantum yield to chemiluminescent resonance energy transfer

    International Nuclear Information System (INIS)

    The resonance energy transfer between chemiluminescence donor (luminol-H2O2 system) and quantum dots (QDs, emission at 593 nm) acceptors (CRET) was investigated. The resonance energy transfer efficiencies were compared while the oil soluble QDs, water soluble QDs (modified with thioglycolate) and QD-HRP conjugates were used as acceptor. The fluorescence of QD can be observed in the three cases, indicating that the CRET occurs while QD acceptor in different status was used. The highest CRET efficiency (10.7%) was obtained in the case of oil soluble QDs, and the lowest CRET efficiency (2.7%) was observed in the QD-HRP conjugates case. This result is coincident with the quantum yields of the acceptors (18.3% and 0.4%). The same result was observed in another similar set of experiment, in which the amphiphilic polymer modified QDs (emission at 675 nm) were used. It suggests that the quantum yield of the QD in different status is the crucial factor to the CRET efficiency. Furthermore, the multiplexed CRET between luminol donor and three different sizes QD acceptors was observed simultaneously. This work will offer useful support for improving the CRET studies based on quantum dots

  19. Study on the interaction of catalase with pesticides by flow injection chemiluminescence and molecular docking.

    Science.gov (United States)

    Tan, Xijuan; Wang, Zhuming; Chen, Donghua; Luo, Kai; Xiong, Xunyu; Song, Zhenghua

    2014-08-01

    The interaction mechanisms of catalase (CAT) with pesticides (including organophosphates: disulfoton, isofenphos-methyl, malathion, isocarbophos, dimethoate, dipterex, methamidophos and acephate; carbamates: carbaryl and methomyl; pyrethroids: fenvalerate and deltamethrin) were first investigated by flow injection (FI) chemiluminescence (CL) analysis and molecular docking. By homemade FI-CL model of lg[(I0-I)/I]=lgK+nlg[D], it was found that the binding processes of pesticides to CAT were spontaneous with the apparent binding constants K of 10(3)-10(5) L mol(-1) and the numbers of binding sites about 1.0. The binding abilities of pesticides to CAT followed the order: fenvalerate>deltamethrin>disulfoton>isofenphos-methyl>carbaryl>malathion>isocarbophos>dimethoate>dipterex>acephate>methomyl>methamidophos, which was generally similar to the order of determination sensitivity of pesticides. The thermodynamic parameters revealed that CAT bound with hydrophobic pesticides by hydrophobic interaction force, and with hydrophilic pesticides by hydrogen bond and van der Waals force. The pesticides to CAT molecular docking study showed that pesticides could enter into the cavity locating among the four subdomains of CAT, giving the specific amino acid residues and hydrogen bonds involved in CAT-pesticides interaction. It was also found that the lgK values of pesticides to CAT increased regularly with increasing lgP, Mr, MR and MV, suggesting that the hydrophobicity and steric property of pesticide played essential roles in its binding to CAT.

  20. An ultrasensitive electrogenerated chemiluminescence-based immunoassay for specific detection of Zika virus

    Science.gov (United States)

    Acharya, Dhiraj; Bastola, Pradip; Le, Linda; Paul, Amber M.; Fernandez, Estefania; Diamond, Michael S.; Miao, Wujian; Bai, Fengwei

    2016-01-01

    Zika virus (ZIKV) is a globally emerging mosquito-transmitted flavivirus that can cause severe fetal abnormalities, including microcephaly. As such, highly sensitive, specific, and cost-effective diagnostic methods are urgently needed. Here, we report a novel electrogenerated chemiluminescence (ECL)-based immunoassay for ultrasensitive and specific detection of ZIKV in human biological fluids. We loaded polystyrene beads (PSB) with a large number of ECL labels and conjugated them with anti-ZIKV monoclonal antibodies to generate anti-ZIKV-PSBs. These anti-ZIKV-PSBs efficiently captured ZIKV in solution forming ZIKV-anti-ZIKV-PSB complexes, which were subjected to measurement of ECL intensity after further magnetic beads separation. Our results show that the anti-ZIKV-PSBs can capture as little as 1 PFU of ZIKV in 100 μl of saline, human plasma, or human urine. This platform has the potential for development as a cost-effective, rapid and ultrasensitive assay for the detection of ZIKV and possibly other viruses in clinical diagnosis, epidemiologic and vector surveillance, and laboratory research. PMID:27554037

  1. A simple and rapid chemiluminescence aptasensor for acetamiprid in contaminated samples: Sensitivity, selectivity and mechanism.

    Science.gov (United States)

    Qi, Yingying; Xiu, Fu-Rong; Zheng, Minfeng; Li, Baoxin

    2016-09-15

    Ultralow concentration and selective detection of pesticide residue is important to evaluate the environmental and biological pollution and the threat to human health which single component pesticide can bring. Herein, we report an amplified chemiluminescence (CL) sensing platform for ultrasensitive and selective acetamiprid (widely used pesticide) detection. It is based on aptamer's high binding affinity to target and the relevance between AuNPs' morphology and its catalytic effect to stimulate the generation of CL in the presence of H2O2 and luminol. Moreover, AuNPs morphological slight change induced by aptamers' conformation during targets binding could lead to the significant change of catalytic properties. Therefore, the proposed sensing platform for pesticide residue exhibited a high sensitivity toward acetamiprid with a detection limit of 62pM, which was about 100-fold lower than that of other aptamer-based sensor for acetamiprid detection. Because of the intrinsic specificity of aptamer's recognization, this sensing platform has high selectivity. So, this sensing platform provides a label-free and cost-effective approach for sensitive and selective detection of single component pesticide residue. More importantly, this CL method was successfully used to determine acetamiprid in real contaminated samples. PMID:27131997

  2. Chemiluminescence imaging for microRNA detection based on cascade exponential isothermal amplification machinery.

    Science.gov (United States)

    Xu, Yongjie; Li, Dandan; Cheng, Wei; Hu, Rong; Sang, Ye; Yin, Yibing; Ding, Shijia; Ju, Huangxian

    2016-09-14

    A novel G-quadruplex DNAzyme-driven chemiluminescence (CL) imaging method was developed for ultrasensitive and specific detection of miRNA based on the cascade exponential isothermal amplification reaction (EXPAR) machinery. A structurally tailored hairpin probe switch was designed to selectively recognise miRNA and form hybridisation products to trigger polymerase and nicking enzyme machinery, resulting in the generation of product I, which was complementary to a region of the functional linear template. Then, the response of the functional linear template to the generated product I further activated the exponential isothermal amplification machinery, leading to synthesis of numerous horseradish peroxidase mimicking DNAzyme units for CL signal transduction. The amplification paradigm generated a linear response from 10 fM to 100 pM, with a low detection limit of 2.91 fM, and enabled discrimination of target miRNA from a single-base mismatched target. The developed biosensing platform demonstrated the advantages of isothermal, homogeneous, visual detection for miRNA assays, offering a promising tool for clinical diagnosis. PMID:27566360

  3. Self-catalytic degradation of ortho-chlorophenol with Fenton's reagent studied by chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    Zhen Lin; HuiChen; Yun Zhou; Nobuaki Ogawa; Jin-Ming Lin

    2012-01-01

    The degradation of ortho-chlorophenol using Fenton's reagent was studied by chemiluminescence(CL).Without a special CL reagent,a weak CL emission from the mixture of ferrous ion and hydrogen peroxide was observed at room temperature.The CL intensity was increased by the addition of ortho-chlorophenol into the mixed solution.When the temperature was raised to 65℃,the CL intensity was enhanced strongly.The CL mechanisms for the system H2O2-Fe2+ with and without ortho-chlorophenol were studied by examining the CL spectrum,gas chromatography-mass spectrometry and electron spin resonance spectrum.The effects of various free radical scavengers,surfactants and fluorescence compounds on the CL intensity were also investigated.A self-catalytic oxidation mechanism was proposed.The results showed that singlet oxygen was the main emitter for the system H2O2-Fe2+.The strong CL from the system H2O2-Fe2+-ortho-chlorophenol was due to singlet oxygen and electronically excited quinone.The benzenediol-like intermediate product formed during the phenol oxidation process greatly promoted the Fenton's reaction and led to higher CL intensity.Chemiluninescence is a novel approach for the investigation of the oxidation of some organic pollutants by Fenton's reagent.

  4. Mapping of explosive contamination using GC/chemiluminescence and ion mobility spectrometry techniques

    Science.gov (United States)

    Miller, Carla J.; Glenn, D. F.; Hartenstein, Steven D.; Hallowell, Susan F.

    1998-12-01

    Recent efforts at the Idaho National Engineering and Environmental Laboratory (INEEL) have included mapping explosive contamination resulting from manufacturing and carrying improvised explosive devices (IEDs). Two types of trace detection equipment were used to determine levels of contamination from designated sampling areas. A total of twenty IEDs were constructed: ten using TNT and ten using C-4. Two test scenarios were used. The first scenario tracked the activities of a manufacturer who straps the device onto an independent courier. The courier then performed a series of activities to simulate waiting in an airport. The second scenario tracked the activities of a manufacturer who also served as the courier. A sample set for each test consisted of thirty samples from various locations on each IED manufacturer, thirty from each IED courier, twenty-five from the manufacturing area, and twenty-five from the courier area. Pre-samples and post-samples were collected for analysis with each detection technique. Samples analyzed by gc/chemiluminescence were taken by swiping a teflon- coated sampling swipe across the surface of the sampling area to pick up any explosive particles. Samples analyzed by ion mobility spectrometry (IMS) were taken from the clothing of the manufacturer and courier by vacuuming the surface and collecting particulates on a fiberglass filter. Samples for IMS analysis from the manufacturing and courier rooms were taken by wiping a cotton sampling swipe across the surface area. Currently, building IEDs and monitoring the explosive contamination is being directed toward detection with portal monitors.

  5. A fast and precise chemiluminescence ozone detector for eddy flux and airborne application

    Directory of Open Access Journals (Sweden)

    A. Zahn

    2011-10-01

    Full Text Available A commercially available dry chemiluminescence (CI instrument for fast and precise measurement of ozone (O3 is specified. The sensitivity is ~9000 counts s−1 per ppbv of ozone. Its precision is entirely determined by the number of photons reaching the detector (being a photomultiplier, i.e. is quantum-noise limited. The relative precision (ΔO3/O3 in % thus follows Poisson statistics and scales with the square root of the measurement frequency f and with the inverse O3 mixing ratio: ΔO3/O3f0.5 · O3−0.5. At typical O3 mixing ratios between 10 and 100 ppbv, the precision is 0.3–1.0% at f = 10 Hz. The maximum measurement frequency is 50 Hz. The mechanical and electronic set-up as well as the instrument performance is described. Recommendations on the adequate inlet tube configuration (inlet tube length, sampling flow and on the way of calibration at stationary ground-based platforms and onboard aircraft are given.

  6. Solvent viscosity influence on the chemiexcitation efficiency of inter and intramolecular chemiluminescence systems.

    Science.gov (United States)

    Khalid, Muhammad; Souza, Sergio P; Ciscato, Luiz F M L; Bartoloni, Fernando H; Baader, Wilhelm J

    2015-07-01

    The effects of the medium viscosity on the chemiexcitation quantum yields of the induced decomposition of 1,2-dioxetanes (highly efficient intramolecular CIEEL system) and the catalyzed decomposition of diphenoyl peroxide and a 1,2-dioxetanone derivative (model systems for the intermolecular CIEEL mechanism, despite their low efficiency) are compared in this work. Quantum yields of the rubrene catalyzed decomposition of diphenoyl peroxide and spiro-adamantyl-1,2-dioxetanone as well as the fluoride induced decomposition of a phenoxy-substituted 1,2-dioxetane derivative are shown to depend on the composition of the binary solvent mixture toluene/diphenyl ether, which possess similar polarity parameters but different viscosities. Correlations of the quantum yield data with the medium viscosity using the diffusional and the frictional (free-volume) models indicate that the induced 1,2-dioxetane decomposition indeed occurs by an entirely intramolecular process and the low efficiency of the intermolecular chemiluminescence systems (catalyzed decomposition of diphenoyl peroxide and 1,2-dioxetanone derivative) is not primarily due to the cage escape of radical ion species. PMID:26067192

  7. New bienzymatic strategy for glucose determination by immobilized-gold nanoparticle-enhanced chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    LIN JieHua; ZHANG Hui; ZHANG ShuSheng

    2009-01-01

    Bienzymatic biosensor for the determination of glucose by flow injection chemiluminescence (CL) de-tection was proposed. Hybrids of gold nanoparticles (GNPs) and chitosan were chosen as the immobi-lization matrix of glucose oxidase (GOD) and horseradish peroxidase (HRP) to fabricate the biosensors with silane-pretreated glass mierobeads. After the enzyme catalyzing oxidation of glucose in GOD biosensor, the produced H2O2 flowed into HRP biosensor to react with luminol. The doped GNPs in chitosan were found to enhance the classical CL reaction of luminol-H2O2-HRP. The CL enhancement was investigated in detail by CL and UV-visible spectrum. Under the optimized experimental conditions, glucose could be determined in a linear range from 0.01 to 6.0 mmol/L with a detection limit of 5.0 μmol/L at 3σ. The accuracy of the proposed method was examined by detecting the glucose level in four clinical serum samples from hospital. The proposed method provides a new alternative to deter-mine glucose.

  8. New bienzymatic strategy for glucose determination by immobilized-gold nanoparticle-enhanced chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Bienzymatic biosensor for the determination of glucose by flow injection chemiluminescence(CL) de-tection was proposed.Hybrids of gold nanoparticles(GNPs) and chitosan were chosen as the immobi-lization matrix of glucose oxidase(GOD) and horseradish peroxidase(HRP) to fabricate the biosensors with silane-pretreated glass microbeads.After the enzyme catalyzing oxidation of glucose in GOD biosensor,the produced H2O2 flowed into HRP biosensor to react with luminol.The doped GNPs in chitosan were found to enhance the classical CL reaction of luminol-H2O2-HRP.The CL enhancement was investigated in detail by CL and UV-visible spectrum.Under the optimized experimental conditions,glucose could be determined in a linear range from 0.01 to 6.0 mmol/L with a detection limit of 5.0 μmol/L at 3σ.The accuracy of the proposed method was examined by detecting the glucose level in four clinical serum samples from hospital.The proposed method provides a new alternative to deter-mine glucose.

  9. DETERMINATION OF TETRACYCLINE AND OXYTETRACYCLINE BY FLOW-INJECTION CHEMILUMINESCENCE METHOD

    Institute of Scientific and Technical Information of China (English)

    Tang Yuhai; Han Xiaonian; Yu Chunling; Sun Yuanyuan; Yao Hong

    2006-01-01

    Objective To establish a rapid and precise continuous flow-injection chemiluminescence method for the determination of tetracycline and oxytetracycline. Methods In NaOH solution, tetracycline and oxytetracycline can sensitize obviously the chemiluminesence (CL) intensity of the reaction of luminol with KIO4, the sensitized CL intensity is proportional to the concentration of tetracycline and oxytetracycline. So, a new flow-injection CL method has been developed. The optimum chemical conditions for the CL reaction were investigated. Results Under the optimized conditions (KIO4 concentration: 1.0×10-5 mol/L; NaOH concentration: 0.1 mol/L; luminol concentration: 1.0×10-4mol/L), tetracycline and oxytetracycline were determined. The linear range of the working curves was 1.0×10-7 -1.0×10-4g/mL, the detection limits was 1.0×10-8g/mL and 1.1×10-8g/mL, and the relative standard deviation was 2.6% (CS=1.0×10-6g/mL; n=11) and 2.0% (CS=1.0×10-6g/mL; n=11) respectively. Conclusion The method is simple, rapid, and sensitive, and it has been successfully applied to the the determination of tetracycline and oxytetracycline tablets, the mean recoveries being 99.7% and 98.8% respectively.

  10. Electrogenerated chemiluminescent behavior of MCLA at an indium-tin-oxide electrode

    Energy Technology Data Exchange (ETDEWEB)

    Chen Zhihuang [MOE Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002 (China); Wang Jian [MOE Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002 (China); Lin Zhengyu [MOE Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002 (China); Chen Guonan [MOE Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002 (China)]. E-mail: gnchen@fzu.edu.cn

    2006-08-15

    In this paper, the electrogenerated chemiluminescence (ECL) behavior of 6-(4-methoxyphenyl)-2-methylimidazo[1,2-a]pyrazin-3(7H)-one (MCLA) at an indium-tin-oxide (ITO) electrode in phosphate buffer solution has been investigated when linear sweep voltammetry was applied. The optimum chemical conditions and electrochemical parameters for this ECL system have been investigated in detail. Under the optimum conditions, it was found that the ECL intensity was linear with the concentration of MCLA in the range of 6.0 x 10{sup -7} to 1.0 x 10{sup -5} mol/L, and its detection limit for MCLA was 2.0 x 10{sup -7} mol/L. The possible mechanism for ECL of MCLA at the ITO electrode was also discussion. Furthermore, it was found that some sophora flavescens, such as matrine and oxymatrine, might enhance the ECL intensity of MCLA. Therefore, it is possible to develop a new ECL method for determination of matrine and oxymatrine.

  11. Ru(bpy)32+-doped Silica Nanoparticle Aptasensor for Detection of Thrombin Based on Electrogenerated Chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    WANG Xiao-Ying; YUN Wen; ZHOU Jing-Ming; DONG Ping; HE Pin-Gang; FANG Yu-Zhi

    2008-01-01

    A new electrogenerated chemiluminescent (ECL) detection system for protein using the aptamers was developed.Two different aptamers, which recognize different positions of thrombin, were chosen to construct sandwich type sensing system for protein, one was immobilized onto the gold electrode for capturing thrombin onto the electrode and the other was used for detection.To obtain the signal, the aptamer for detection was labeled with tris(2,2'-bipyridyl)ruthenium(Ⅱ)-doped silica nanoparticles ( Ru(bpy)32+-doped SNPs).The increase of the ECL signal generated by Ru(bpy)32+-doped SNPs was observed in dependent manner on the concentration of thrombin added.Bovine serum albumin and bovine hemoglobin had almost negligible responses.The assay allows detection at levels as low as 1.0 fmol·L-1 of the thrombin due to that there are a large number of Ru(bpy)32+ molecules inside SNPs labeled on the aptamer used for detection.The ECL signal was linearly related to the concentration of the thrombin analyte in the range of 2.0 fmol·L-1 to 2.0 pmol·L-1.

  12. Determination of Melatonin in Rat Pineal Gland and Drug with Flow-Injection Chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    WEI,Shou-Lian; ZHAO,LI-Xia; CHENG,Xiang-Lei; LIN,Jin-Ming

    2007-01-01

    A flow-injection chemiluminescence(CL)method for the determination of melatonin based on the CL reaction of melatonin with hydrogen peroxide and sodium hypochlorite(NaOCl)in a basic alkaline solution was developed.The possible CL mechanism has been discussed,and a proposal for the reaction pathway was given that singlet oxygen was clarified to be produced in this reaction system and was responsible for the CL emission.Under the optimized conditions.the linear concentration range of application was 1.0 X 10-7-2.5×10-4 mol·L-1 with a detection Iimit of 5.0X 10-8 mol·L-1(S/N=3).The relative standard deviation for eight repeated measurements of 1.0×10-6 mol·L-1 melatonin was 2.8%.The interferences of several important biological substances.some indole compound,cations and anions were studied.No interference was found for the anions,glucose,starch,most of cations and low concentration(less than 3.0×10-6 mol·L-1)of some biological substances and indole compound.The method was applied to the determination of melatonin in rat pineal gland and drug with satisfactory results.The sample throughput was 90 injections per hour.

  13. Trace analysis of pollutants by use of honeybees, immunoassays, and chemiluminescence detection.

    Science.gov (United States)

    Girotti, S; Ghini, S; Maiolini, E; Bolelli, L; Ferri, E N

    2013-01-01

    Specific and sensitive analysis to reveal and monitor the wide variety of chemical contaminants polluting all environment compartments, feed, and food is urgently required because of the increasing attention devoted to the environment and health protection. Our research group has been involved in monitoring the presence and distribution of agrochemicals by monitoring beehives distributed throughout the area studied. Honeybees have been used both as biosensors, because the pesticides affect their viability, and as "contaminant collectors" for all environmental pollutants. We focused our research on the development of analytical procedures able to reveal and quantify pesticides in different samples but with a special attention to the complex honeybee matrix. Specific extraction and purification procedures have been developed and some are still under optimization. The analytes of interest were determined by gas or liquid chromatographic methods and by compound-specific or group-specific immunoassays in the ELISA format, the analytical performance of which was improved by introducing luminescence detection. The range of chemiluminescent immunoassays developed was extended to include the determination of completely different pollutants, for example explosives, volatile organic compounds (including benzene, toluene, ethylbenzene, xylenes), and components of plastics, for example bisphenol A. An easier and portable format, a lateral flow immunoassay (LFIA) was added to the ELISA format to increase application flexibility in these assays. Aspects of the novelty, the specific characteristics, the analytical performance, and possible future development of the different chromatographic and immunological methods are described and discussed. PMID:23064670

  14. A simple inexpensive gas phase chemiluminescence analyzer for measuring trace levels of arsenic in drinking water

    Energy Technology Data Exchange (ETDEWEB)

    Sengupta, Mrinal K.; Hossain, Zafreen A.; Ohira, Shin-Ichi [Department of Chemistry and Biochemistry, University of Texas at Arlington, 700 Planetarium Place, Arlington, TX 76019-0065 (United States); Dasgupta, Purnendu K., E-mail: dasgupta@uta.ed [Department of Chemistry and Biochemistry, University of Texas at Arlington, 700 Planetarium Place, Arlington, TX 76019-0065 (United States)

    2010-01-15

    An inexpensive sensitive gas-phase chemiluminescence (GPCL) based analyzer for arsenic is described; this device utilizes manual fluid dispensing operations to reduce size, weight and cost. The analyzer in its present form has a limit of detection (LOD, S/N = 3) of 1.0 mug/L total inorganic As (peak heightbased, 3 mL sample). The system was used to measure low level arsenic in tap water samples from Texas and New Mexico and compared with results obtained by inductively coupled plasma-mass spectrometry (ICP-MS) as well as those from an automated GPCL analyzer. Good correlations were observed. Higher levels of As (50-500 mug/L, As(III), As(V) and mixtures thereof) were spiked into local tap water; the recoveries ranged from 95 +- 2% to 101 +- 1%. A single instrument weighs less than 3 kg, consumes <25 W in power, can be incorporated in a briefcase and constructed for <$US $1000. It is easily usable in the field. - An inexpensive instrument capable of measuring down to 1 mug/L As is reported.

  15. Automatic chemiluminescence-based determination of carbaryl in various types of matrices.

    Science.gov (United States)

    Pulgarín, José Antonio Murillo; Molina, Aurelia Alañón; López, Pablo Fernández

    2006-01-15

    Carbaryl, a modern pesticide widely used for both agricultural and non-agricultural purposes, was determined from the chemiluminescence produced in its reaction with Ce(IV) in a nitric acid medium containing rhodamine 6G as sensitizer, using flow-injection techniques. A straightforward automatic method based on measurements peak height and peak area, which are directly proportional to the carbaryl concentration, was thus developed. Calibration graphs are linear over the concentration range from 50 to 2000 ng mL(-1). The limit of detection, as determined according to Clayton, is 45.6 and 28.7 ng mL(-1) for peak height and peak area measurements, respectively. The relative standard deviation for 10 samples was less than 1.4% with both types of measurements. Two commercial formulations containing carbaryl were analysed using both types of measurements, which provided acceptable recovery values. Solid-phase extraction was used to concentrate and separate the analyte from the matrix. The method was successfully applied to the analysis of spiked water samples as well as in soil and grain samples. The proposed method exhibited a high selectivity no other pesticide containing the naphthalene group such as antu, napropamide or naftalam, etc., was found to interfere with the determination of carbaryl. PMID:18970361

  16. Determination of Prometryn in Vetiver Grass and Water Using Gas Chromatography-Nitrogen Chemiluminescence Detection.

    Science.gov (United States)

    Sun, Shixian; Li, Yongmei; Lv, Ping; Punamiya, Pravin; Sarkar, Dibyendu; Dan, Youming; Ma, Junrong; Zheng, Yi

    2016-02-01

    Nitrogen chemiluminescence detector (NCD) is a nitrogen-specific detector that responds to ammonia, hydrazine, hydrogen cyanide and nitrogen oxide. A method to analyze the herbicide prometryn in plant and water samples was developed using gas chromatograph (GC) coupled with NCD. Extracts from plant (vetiver grass) and water matrices were analyzed for prometryn using an Agilent 7890A GC coupled with an Agilent 255 NCD in a split injection mode with a ratio of 2 : 1. Separation was carried out at 200°C and combustion at 1,018°C with H2 and O2 following optimized method development conditions. The percent recovery of prometryn in the two different matrices tested ranged from 81 to 107%, with relative standard deviations varying from 0.10 to 3.30% for spiked samples. Detection limit of the proposed method was 0.02 µg mL(-1) and the limit of quantification was 0.06 µg mL(-1). The proposed GC-NCD method was successfully applied to determine prometryn extracted from plant and water samples without potential interference of S-triazine, a pesticide from the same group.

  17. Chemiluminescence behavior of sodium hydrogen carbonate in the potassium permanganate-hydrogen peroxide reaction

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Chemiluminescence (CL) phenomenon of hydrogen peroxide with potassium permanganate in the presence of sodium hydrogen carbonate was reported.Effects of the surfactant on the CL system were investigated.Nonionic surfactants could effectively increase the CL signal.Radical scavengers and organic reagents such as nitro blue tetrazolium chloride (NBT),cytochrome c,sodium azide,ascorbic acid,thiourea,tert-butanol and dimethyl sulphoxide were used to study the emitting species.CL emission spectrum was recorded and the results showed that the maximal emission wavelengths of NaHCO3-H2O2-KMnO4 system were 440 and 634 nm.The mechanism was discussed based on electron spin resonance (ESR) spectra,fluorescence spectra and UV-vis absorption spectra.The addition of rhodamine B or uranine into this CL system enhanced the CL signal.It was due to part of the energy transfer from singlet oxygen and excited triplet dimers of two CO2 molecules to rhodamine B or uranine.The CL could be induced by excited rhodamine B or uranine.

  18. The study of chemiluminescence immunoassay for determination of human serum true insulin

    International Nuclear Information System (INIS)

    To develop a highly sensitive CLIA to detect human serum true insulin, two specific monoclonal antibodies having different and distinct epitopes on insulin molecule were used in this study: one was coated on microtiter plate as the solid phase antibody and the other was labeled with alkaline phosphatase. Adamantine derivate was used as the substrate. The results showed that the sensitivity was 0.06 μIU/mL, and the linear calibrator was in the range of 1.0-150 μIU/mL. The CV of intra-and interbatches were 5.0% and 7.8%, respectively, and the mean recovery rate was 94.4%. According to measurement results of 200 samples (100 men and 100 women) the determination range was 0.52%-11.23 μIU/mL for males, 0.75-10.66 μIU/mL for females, and the mean value was 3.86, 3.62 μIU/mL. There was no obvious difference between men and women. Chemiluminescence immunoassay (CLIA) is a simple and convenient, and can reflect truly the level of serum insulin. CLIA of insulin has application value in diagnosis and pathological research of diabetes mellitus. (authors)

  19. The Chemiluminescence and Structure Properties of Normal/Inverse Diffusion Flames

    Directory of Open Access Journals (Sweden)

    Ting Zhang

    2013-01-01

    Full Text Available The flame emission spectrometry was applied to detect the distribution of excited radicals in two types CH4/O2 coflow jet diffusion flames (normal and inverse diffusion flames. Combining the image analysis along with the spectrometry, the chemiluminescence and structure characteristics of these diffusion flames were investigated. The results show that the inverse diffusion flame (IDF with relatively high inlet oxygen velocity is composed of two regions: a bright base and a tower on top of the base, which is quite different from the normal diffusion flame (NDF. The flame is divided into two regions along the flame axis based on maximum OH* position (Region I: initial reaction zone; Region II: further oxidation zone. The degree of the further oxidization taking place in Region II is obvious in accordance with OH* distribution, which is the main difference in reaction zone between fuel-rich condition and fuel-lean condition for NDFs. For IDFs, the change of OH* distribution with increasing equivalence O/C ratio ([O/C]e in Region II is not conspicuous. More OH* and CH* are generated in IDFs, due to the inner high-speed O2 flow promoting the mixing of fuel and oxygen to a certain extent.

  20. An ultrasensitive electrogenerated chemiluminescence-based immunoassay for specific detection of Zika virus.

    Science.gov (United States)

    Acharya, Dhiraj; Bastola, Pradip; Le, Linda; Paul, Amber M; Fernandez, Estefania; Diamond, Michael S; Miao, Wujian; Bai, Fengwei

    2016-01-01

    Zika virus (ZIKV) is a globally emerging mosquito-transmitted flavivirus that can cause severe fetal abnormalities, including microcephaly. As such, highly sensitive, specific, and cost-effective diagnostic methods are urgently needed. Here, we report a novel electrogenerated chemiluminescence (ECL)-based immunoassay for ultrasensitive and specific detection of ZIKV in human biological fluids. We loaded polystyrene beads (PSB) with a large number of ECL labels and conjugated them with anti-ZIKV monoclonal antibodies to generate anti-ZIKV-PSBs. These anti-ZIKV-PSBs efficiently captured ZIKV in solution forming ZIKV-anti-ZIKV-PSB complexes, which were subjected to measurement of ECL intensity after further magnetic beads separation. Our results show that the anti-ZIKV-PSBs can capture as little as 1 PFU of ZIKV in 100 μl of saline, human plasma, or human urine. This platform has the potential for development as a cost-effective, rapid and ultrasensitive assay for the detection of ZIKV and possibly other viruses in clinical diagnosis, epidemiologic and vector surveillance, and laboratory research. PMID:27554037

  1. Flow Injection Analysis with Chemiluminescence detection for Determination of Two Phenothiazines

    Directory of Open Access Journals (Sweden)

    Hijran S. Jabbar

    2015-03-01

    Full Text Available A new flow injection analysis-chemiluminescence (FIA-CL method for determination of two phenothiazine derivatives "chlorpromazine hydrochloride (CPZ and promethazine hydrochloride (PTZ" described. The redox reaction between Fe(III and phenothiazines was taken as a base of this new method. A well-defined volume of CPZ or PTZ and luminol solution is injected simultaneously by means of two injection valves in accordance with the merging zone principle. Calibration graphs were constructed in the range of (0.05 – 1.4μg/ml for CPZ with correlation coefficient of (0.9972 and in the range of (0.5 – 40μg/ml for PTZ with correlation coefficient of (0.9978. The precision and accuracy of the method were checked by calculation of relative standard deviation (RSD and relative error percentage (%E for two different levels of concentrations. The method was relatively free from common excipients and it is applied successfully for the determination of CPZ and PTZ in pharmaceutical formulations. The results obtained were in good agreement with those obtained by a reference method in British Pharmacopoeia.

  2. Chemiluminescence of neutrophiles stimulated by opsonized Zymosan in children with bronchial asthma and pneumonia

    Science.gov (United States)

    Lewandowicz-Uszynska, A.; Jankowski, A.

    2004-08-01

    Oxygen metabolism of neutrophils after stimulation with opsonized zymosan was examined using chemiluminescence test (in the presence of the patient serum or pooled serum). Into the study 37 children aged from 2 to 12 years were enrolled (20 girls and 17 boys). 10 healthy volunteers comprised the control group (group III). Two groups of patients were established: group I -- children with bronchial asthma (without infection), group II -- children with pneumonia. The examination in both groups was performed twice -- in acute phase and in remission period. The group I in acute phase comprised 16 children and in remission phase 9 children, group II - 21 children in acute phase and 9 children in remission phase, respectively. The following parameters of CL were estimated average value of so called spontaneous CL, maximal excitation of neutrophils after stimulation by zymogen (CLmax), time of zymosan opsonization. The following results were obtained: increased spontaneous CL and CLmax (at the presence of both sera) in acute phase of bronchial asthma and pneumonia in comparison to the control group. In the period of remission both these parameters were insignificantly decreased. The longest time of zymosan opsonization in acute period of disease was observed in children with pneumonia (18 min.). This time did not change during remission phase. Only slightly longer time of opsonization was observed in the patients from group I (in exacerbation) (15 min) than in the control group (13,1 min). This time was prolonged in the clinical remission (20 min).

  3. Comparison of ultraviolet absorbance, chemiluminescence, and DOAS instruments for ambient ozone monitoring.

    Science.gov (United States)

    Williams, E J; Fehsenfeld, F C; Jobson, B T; Kuster, W C; Goldan, P D; Stutz, J; McClenny, W A

    2006-09-15

    This paper evaluates the accuracy of ozone measurements made by monitors that determine ozone concentrations in ambient air by UV absorption. These monitors are typically used to measure ozone for the purpose of establishing local compliance to air-quality standards. The study was predicated by the concern that commercially available UV absorbance O3 monitors may be subject to interference from volatile organic carbon (VOC) species that absorb light at 254 nm. To test for these and other effects, we compared simultaneous O3 measurements made by a commercial UV O3 monitor with an O3-NO chemiluminescence instrument, which is not subject to interference by VOC compounds. The comparisons were carried out in the summers of 1999 and 2000 at urban/industrial sites in Nashville and Houston, and in 2004 aboard a ship in the Gulf of Maine. In the two urban areas, we also compared the 03 measurements from these two methods with O3 measurements made by a long-path differential optical absorption spectrometer (DOAS). Our tests indicate that, with well-maintained monitors, there are no significant interferences even in areas with significant ambient concentrations of potentially interfering VOCs. PMID:17007137

  4. Highly sensitive chemiluminescence technology for protein detection using aptamer-based rolling circle amplification platform

    Institute of Scientific and Technical Information of China (English)

    Zhi-Juan Cao; Qian-Wen Peng; Xue Qiu; Cai-Yun Liu; Jian-Zhong Lu

    2011-01-01

    A robust, selective and highly sensitive chemiluminescent (CL) platform for protein assay was presented in this paper. This novel CL approach utilized rolling circle amplification (RCA) as a signal enhancement technique and the 96-well plate as the immobilization and separation carrier. Typically, the antibody immobilized on the surface of 96-well plate was sandwiched with the protein target and the aptamer-primer sequence. This aptamer-primer sequence was then employed as the primer of RCA. Based on this design, a number of the biotinylated probes and streptavidin-horseradish peroxidase (SA-HRP) were captured on the plate, and the CL signal was amplified. In summary, our results demonstrated a robust biosensor with a detection limit of 10 fM that is easy to be established and utilized, and devoid of light source. Therefore, this new technique .will broaden the perspective for future development of DNA-based biosensors for the detection of other protein biomarkers related to clinical diseases, by taking advantages of high sensitivity and selectivity.

  5. Chemiluminescence as a condition monitoring method for thermal aging and lifetime prediction of an HTPB elastomer.

    Energy Technology Data Exchange (ETDEWEB)

    Gillen, Kenneth Todd; Minier, Leanna M. G.; Celina, Mathias Christopher; Trujillo, Ana B.

    2007-03-01

    Chemiluminescence (CL) has been applied as a condition monitoring technique to assess aging related changes in a hydroxyl-terminated-polybutadiene based polyurethane elastomer. Initial thermal aging of this polymer was conducted between 110 and 50 C. Two CL methods were applied to examine the degradative changes that had occurred in these aged samples: isothermal 'wear-out' experiments under oxygen yielding initial CL intensity and 'wear-out' time data, and temperature ramp experiments under inert conditions as a measure of previously accumulated hydroperoxides or other reactive species. The sensitivities of these CL features to prior aging exposure of the polymer were evaluated on the basis of qualifying this method as a quick screening technique for quantification of degradation levels. Both the techniques yielded data representing the aging trends in this material via correlation with mechanical property changes. Initial CL rates from the isothermal experiments are the most sensitive and suitable approach for documenting material changes during the early part of thermal aging.

  6. A fast and precise chemiluminescence ozone detector for eddy flux and airborne application

    Directory of Open Access Journals (Sweden)

    A. Zahn

    2012-02-01

    Full Text Available A commercially available dry chemiluminescence (CI instrument for fast and precise measurement of ozone (O3 is specified. The sensitivity is ~9000 counts s−1 per ppbv of ozone. Its precision is entirely determined by the number of photons reaching the detector (being a photomultiplier, i.e. is quantum-noise limited. The relative precision (ΔO3/O3 in % thus follows Poisson statistics and scales with the square root of the measurement frequency f and with the inverse O3 mixing ratio: ΔO3/O3f0.5 · O3−0.5. At typical O3 mixing ratios between 10 and 100 ppbv (and 1 bar, the precision is 0.3–1.0% at f = 10 Hz. The maximum measurement frequency is 50 Hz. The mechanical and electronic set-up as well as the instrument performance is described. Recommendations on the adequate inlet tube configuration (inlet tube length, sampling flow and on the way of calibration at stationary ground-based platforms and onboard aircraft are given.

  7. A simple inexpensive gas phase chemiluminescence analyzer for measuring trace levels of arsenic in drinking water

    International Nuclear Information System (INIS)

    An inexpensive sensitive gas-phase chemiluminescence (GPCL) based analyzer for arsenic is described; this device utilizes manual fluid dispensing operations to reduce size, weight and cost. The analyzer in its present form has a limit of detection (LOD, S/N = 3) of 1.0 μg/L total inorganic As (peak heightbased, 3 mL sample). The system was used to measure low level arsenic in tap water samples from Texas and New Mexico and compared with results obtained by inductively coupled plasma-mass spectrometry (ICP-MS) as well as those from an automated GPCL analyzer. Good correlations were observed. Higher levels of As (50-500 μg/L, As(III), As(V) and mixtures thereof) were spiked into local tap water; the recoveries ranged from 95 ± 2% to 101 ± 1%. A single instrument weighs less than 3 kg, consumes <25 W in power, can be incorporated in a briefcase and constructed for <$US $1000. It is easily usable in the field. - An inexpensive instrument capable of measuring down to 1 μg/L As is reported.

  8. Study on the Inclusion Behavior of Sulfobutylether-β-Cyclodextrin with Perphenazine by Flow Injection Chemiluminescence

    Energy Technology Data Exchange (ETDEWEB)

    Shen, Minxia; Lv, Hairu; Song, Zhenghua [Northwest Univ., Xi' an (China)

    2013-11-15

    The inclusion behavior of sulfobutylether-β-cyclodextrin (SBE-β-CD) with perphenazine (PPH) was first studied by flow injection (FI)-chemiluminescence (CL) analysis with proposed lg[(I{sub 0} - I{sub s})/I{sub s}] = lgK{sub P-CD} + nlg[C{sub PPH}] model and molecular docking. Results showed that a 1:1 complex of SBE-β-CD/PPH could online form, with the formation constant K{sub P-CD} of 2.57 Χ 10{sup 7} L mol{sup -1} at 298 K. The thermodynamic parameters showed that the inclusion behavior of SBE-β-CD/PPH was a spontaneous process by hydrophobic interaction. The molecular docking results revealed PPH entered into the larger cavity of SBE-β-CD with two hydrogen bonds. Based on the linear relationship of the decrement of luminol/SBE-β-CD/PPH CL intensity against the logarithm of PPH concentration ranging from 0.03 to 30.0 ng mL{sup -1}, the present FI-CL analysis using luminol/SBE-β-CD/PPH system was successfully applied to PPH determination in biological fluids and tablets with recoveries from 94.5 to 105.6% and RSDs less than 2.6% (n = 5)

  9. Polydimethylsiloxane microfluidic chemiluminescence immunodevice with the signal amplification strategy for sensitive detection of human immunoglobin G.

    Science.gov (United States)

    Li, Huifang; Zhao, Mei; Liu, Wei; Chu, Weiru; Guo, Yumei

    2016-01-15

    A polydimethylsiloxane (PDMS) microfluidic chemiluminescence (CL) immunodevice for sensitive detection of human immunoglobin G (IgG) with the signal amplification strategy was developed in this work. The immunodevice was prepared by covalently immobilizing capture antibodies (Abs) on the silanized microchannel of microfluidic chip. Gold nanoparticles (AuNPs) functionalized with a high molar ratio of horseradish peroxidase (HRP) were used as an Ab label for signal amplification. Using a sandwich immunoassay, the multi-HRP conjugated AuNPs can catalyze the luminol-H2O2 CL system to achieve the high sensitivity. In addition, the double spiral flow-channel was adopted here, which can still contribute to the high sensitivity. Based on signal amplification strategy, the performance of human IgG tests revealed a lower detection limit (DL) of 0.03ng/mL and showed an increase of 7.4-fold in detection sensitivity compared to a commercial Ab-HRP conjugation. This microfluidic immunodevice can provide an alternative approach for sensitive detection of human IgG in the field of clinic diagnostic and therapeutic. PMID:26592629

  10. Chemiluminescence imaging for microRNA detection based on cascade exponential isothermal amplification machinery.

    Science.gov (United States)

    Xu, Yongjie; Li, Dandan; Cheng, Wei; Hu, Rong; Sang, Ye; Yin, Yibing; Ding, Shijia; Ju, Huangxian

    2016-09-14

    A novel G-quadruplex DNAzyme-driven chemiluminescence (CL) imaging method was developed for ultrasensitive and specific detection of miRNA based on the cascade exponential isothermal amplification reaction (EXPAR) machinery. A structurally tailored hairpin probe switch was designed to selectively recognise miRNA and form hybridisation products to trigger polymerase and nicking enzyme machinery, resulting in the generation of product I, which was complementary to a region of the functional linear template. Then, the response of the functional linear template to the generated product I further activated the exponential isothermal amplification machinery, leading to synthesis of numerous horseradish peroxidase mimicking DNAzyme units for CL signal transduction. The amplification paradigm generated a linear response from 10 fM to 100 pM, with a low detection limit of 2.91 fM, and enabled discrimination of target miRNA from a single-base mismatched target. The developed biosensing platform demonstrated the advantages of isothermal, homogeneous, visual detection for miRNA assays, offering a promising tool for clinical diagnosis.

  11. An ultrasensitive chemiluminescence biosensor for carcinoembryonic antigen based on autocatalytic enlargement of immunogold nanoprobes.

    Science.gov (United States)

    Hao, Minjia; Ma, Zhanfang

    2012-01-01

    A sensitive flow injection chemiluminescence assay for carcinoembryonic antigen (CEA) detection based on signal amplification with gold nanoparticles (NPs) is reported in the present work. The sandwich system of CEA/anti-CEA/goat-anti-mouse IgG functionalized Au nanoparticles was used as the sensing platform. In order to improve detection sensitivity, a further gold enlargement step was developed based on the autocatalytic Au deposition of gold nanoprobes via the reduction of AuCl(4)- to Au0 on their surface in the presence of NH(2)OH·HCl. AuCl(4)-, which is a soluble product of gold nanoprobes, served as an analyte in the CL reaction for the indirect measurement of CEA. Under optimized conditions, the CL intensity of the system was linearly related to the logarithm of CEA concentration in the range of 100 pg∙mL-1 to 1,000 ng∙mL-1, with a detection limit of 20 pg∙mL-1. PMID:23443399

  12. An Ultrasensitive Chemiluminescence Biosensor for Carcinoembryonic Antigen Based on Autocatalytic Enlargement of Immunogold Nanoprobes

    Directory of Open Access Journals (Sweden)

    Minjia Hao

    2012-12-01

    Full Text Available A sensitive flow injection chemiluminescence assay for carcinoembryonic antigen (CEA detection based on signal amplification with gold nanoparticles (NPs is reported in the present work. The sandwich system of CEA/anti-CEA/goat-anti-mouse IgG functionalized Au nanoparticles was used as the sensing platform. In order to improve detection sensitivity, a further gold enlargement step was developed based on the autocatalytic Au deposition of gold nanoprobes via the reduction of AuCl4− to Au0 on their surface in the presence of NH2OH·HCl. AuCl4−, which is a soluble product of gold nanoprobes, served as an analyte in the CL reaction for the indirect measurement of CEA. Under optimized conditions, the CL intensity of the system was linearly related to the logarithm of CEA concentration in the range of 100 pg∙mL−1 to 1,000 ng∙mL−1, with a detection limit of 20 pg∙mL−1.

  13. Real time imaging of live cell ATP leaking or release events by chemiluminescence microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yun

    2008-12-18

    The purpose of this research was to expand the chemiluminescence microscopy applications in live bacterial/mammalian cell imaging and to improve the detection sensitivity for ATP leaking or release events. We first demonstrated that chemiluminescence (CL) imaging can be used to interrogate single bacterial cells. While using a luminometer allows detecting ATP from cell lysate extracted from at least 10 bacterial cells, all previous cell CL detection never reached this sensitivity of single bacteria level. We approached this goal with a different strategy from before: instead of breaking bacterial cell membrane and trying to capture the transiently diluted ATP with the firefly luciferase CL assay, we introduced the firefly luciferase enzyme into bacteria using the modern genetic techniques and placed the CL reaction substrate D-luciferin outside the cells. By damaging the cell membrane with various antibacterial drugs including antibiotics such as Penicillins and bacteriophages, the D-luciferin molecules diffused inside the cell and initiated the reaction that produces CL light. As firefly luciferases are large protein molecules which are retained within the cells before the total rupture and intracellular ATP concentration is high at the millmolar level, the CL reaction of firefly luciferase, ATP and D-luciferin can be kept for a relatively long time within the cells acting as a reaction container to generate enough photons for detection by the extremely sensitive intensified charge coupled device (ICCD) camera. The result was inspiring as various single bacterium lysis and leakage events were monitored with 10-s temporal resolution movies. We also found a new way of enhancing diffusion D-luciferin into cells by dehydrating the bacteria. Then we started with this novel single bacterial CL imaging technique, and applied it for quantifying gene expression levels from individual bacterial cells. Previous published result in single cell gene expression quantification

  14. Real time imaging of live cell ATP leaking or release events by chemiluminescence microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Yun [Iowa State Univ., Ames, IA (United States)

    2008-12-18

    The purpose of this research was to expand the chemiluminescence microscopy applications in live bacterial/mammalian cell imaging and to improve the detection sensitivity for ATP leaking or release events. We first demonstrated that chemiluminescence (CL) imaging can be used to interrogate single bacterial cells. While using a luminometer allows detecting ATP from cell lysate extracted from at least 10 bacterial cells, all previous cell CL detection never reached this sensitivity of single bacteria level. We approached this goal with a different strategy from before: instead of breaking bacterial cell membrane and trying to capture the transiently diluted ATP with the firefly luciferase CL assay, we introduced the firefly luciferase enzyme into bacteria using the modern genetic techniques and placed the CL reaction substrate D-luciferin outside the cells. By damaging the cell membrane with various antibacterial drugs including antibiotics such as Penicillins and bacteriophages, the D-luciferin molecules diffused inside the cell and initiated the reaction that produces CL light. As firefly luciferases are large protein molecules which are retained within the cells before the total rupture and intracellular ATP concentration is high at the millmolar level, the CL reaction of firefly luciferase, ATP and D-luciferin can be kept for a relatively long time within the cells acting as a reaction container to generate enough photons for detection by the extremely sensitive intensified charge coupled device (ICCD) camera. The result was inspiring as various single bacterium lysis and leakage events were monitored with 10-s temporal resolution movies. We also found a new way of enhancing diffusion D-luciferin into cells by dehydrating the bacteria. Then we started with this novel single bacterial CL imaging technique, and applied it for quantifying gene expression levels from individual bacterial cells. Previous published result in single cell gene expression quantification

  15. Confined chemiluminescence detection of nanomolar levels of H2O2 in a paper-plastic disposable microfluidic device using a smartphone.

    Science.gov (United States)

    Lebiga, Elise; Edwin Fernandez, Renny; Beskok, Ali

    2015-08-01

    We report the design and characterization of a disposable light shielded paper-plastic microfluidic device that can detect nanomolar levels of H2O2 using a smartphone camera and a light sealed accessory. Chemiluminescence reaction of H2O2 with bis(2,4,6-trichlorophenyl)oxalate in the presence of rubrene and imidazole is confined to a paper reaction site where the individual reagents are delivered via plastic microfluidic channels. The net photon emission from the chemiluminescence reactions, detected by using a smartphone, is correlated with H2O2 concentrations. With a total reagent volume of 25 μL, the sensor system was able to detect H2O2 concentrations as low as 250 nM. The smartphone based chemiluminescence sensing system has great potential as a point of care diagnostic tool for monitoring nanomolar levels of H2O2 in biological samples. PMID:26057240

  16. Determination of manganese- and manganese-containing fungicides with lucigenin-Tween-20-enhanced chemiluminescence detection.

    Science.gov (United States)

    Yaqoob, Mohammad; Asghar, Mohammad; Nabi, Abdul

    2015-11-01

    A flow-injection (FI) method is reported for the determination of Mn(II), maneb and mancozeb fungicides based on the catalytic effect of Mn(II) on the oxidation of lucigenin and dissolved oxygen in a basic solution. The Tween-20 surfactant has been reported for first time to enhance lucigenin chemiluminescence (CL) intensity in the presence of Mn(II) (53%) and maneb and mancozeb (89%). The calibration graphs were linear in the concentration range of 0.001-1.5 mg L(-1) (R(2) = 0.9982 (n = 11) with a limit of detection (S/N = 3) of 0.1 µg L(-1) for Mn(II) and 0.01-3.0 mg L(-1) [R(2) = 0.9989 and R(2) = 0.9992 (n = 6)] with a limit of detection (S/N =3) of 1.0 µg L(-1) for maneb and mancozeb respectively. Injection throughputs of 90 and 120 h(-1) for Mn(II) and maneb and mancozeb respectively, and relative standard deviations of 1.0-3.4% were obtained in the concentration range studied. The experimental variables, e.g., reagents concentrations, flow rates, sample volume, and photomultiplier tube voltage, were optimized and potential interferences were investigated. The analysis of Mn(II) in river water reference materials (SLRS-4 and SLRS-5) showed good agreement with the certified values incorporating an on-line 8-hydroxyquinoline chelating column in the manifold for removing interfering metal ions. Recoveries for maneb and mancozeb were in the range of 92 ± 5 to 104 ± 3% and 91 ± 2 to 100 ± 4% (n = 3) respectively. The effect of 30 other pesticides (fungicides, herbicides and insecticides) was also examined in the lucigenin-Tween-20 CL system. PMID:25640332

  17. Dye-sensitized phototransformation of chlorophenols and their subsequent chemiluminescence reactions

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Junli [School of Chemical and Material Engineering, Jiangnan University, 1800 Lihu Road, Wuxi 214122 (China); Song Qijun [School of Chemical and Material Engineering, Jiangnan University, 1800 Lihu Road, Wuxi 214122 (China)], E-mail: qsong@jiangnan.edu.cn; Hu Xia; Zhang Enhui; Gao Hui [School of Chemical and Material Engineering, Jiangnan University, 1800 Lihu Road, Wuxi 214122 (China)

    2008-12-15

    A novel chemiluminescence (CL) reaction of chlorophenols (CPs), including 2-chlorophenol (2-CP), 4-CP, 2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP) and pentachlorophenol (PCP) was reported, which was based on the oxidation of the phototransformed CPs by N-bromosuccinimide (NBS). It was found that the dye-sensitized phototransformation is a prerequisite for the subsequent CL reaction, and the presence of 1.9x10{sup -2} mol L{sup -1} Triton X-100 or 3.7x10{sup -3} mol L{sup -1} CTAB can greatly enhance the CL intensity. A neutral sample solution with the presence of 2x10{sup -5} mol L{sup -1} fluorescein (FL) was found to be optimum for the phototransformation of 2-CP, 4-CP, 2,4-DCP and PCP, but a lower pH of 5.3 was more suitable for 2,4,6-TCP. Based on the CL reaction, detection limits of 8.6x10{sup -8}, 1.1x10{sup -7}, 1.5x10{sup -7}, 4.6x10{sup -8} and 3.0x10{sup -5} mol L{sup -1} were achieved, respectively, for 2-CP, 4-CP, 2,4-DCP, 2,4,6-TCP and PCP with the optimized conditions in the flow system. The mechanism of the phototransformation and the subsequent CL reaction were preliminarily studied and it was suggested that the singlet oxygen formed in the dye-sensitization process was responsible for the conversion of CPs into light-emitting precursors. These intermediate products were suggested to be peroxide compounds after testing by a luminal-based post-column CL detection experiment.

  18. Single-layer graphene as a stable and transparent electrode for nonaqueous radical annihilation electrogenerated chemiluminescence.

    Science.gov (United States)

    Cristarella, Teresa C; Chinderle, Adam J; Hui, Jingshu; Rodríguez-López, Joaquín

    2015-04-01

    We explored the use of single-layer graphene (SLG) obtained by chemical vapor deposition, and transferred to a glass substrate, as a transparent electrode material for use in coupled electrochemical and spectroscopic experiments in nonaqueous media through electrogenerated chemiluminescence (ECL). SLG was used with classical ECL luminophores, rubrene and 9,10-diphenylanthracene, in an inert environment to generate stable electrochemical responses and measure light emission through it. As an electrode material, SLG displayed excellent stability during electrochemical potential stepping and voltammetry in a window that spanned at least from ca. -2.4 to +1.8 V versus SCE in acetonitrile and acetonitrile/benzene. Although the peak splitting between forward and reverse sweeps in voltammetry was larger in comparison to metal electrodes due to in-plane resistance, SLG displayed sufficiently facile electron transfer properties to yield stable voltammetric cycling and ECL. SLG electrodes patterned with poly tetrafluoroethylene permitted the stable generation of radical ions on an SLG microelectrode to be studied through scanning electrochemical microscopy in the generation/collection mode. SLG was able to stably collect radical ions produced by a 50 μm gold tip with up to 96% collection efficiency. The transparency of graphene was used to obtain accurate spectral responses in ECL. While inner filter effects are known to cause a shift in peak emission wavelength of spectroelectrochemical studies, the use of SLG electrodes with detection through the graphene window reduced apparent peak shifts by up to 10 nm in peak wavelength. This work introduces SLG as a virtually transparent, electrochemically active, and chemically stable platform for studying ECL in the radical annihilation mode, where large electrode polarizations could compromise the chemical stability of other existing transparent electrodes. PMID:25780938

  19. Spectroscopic studies on the lanthanide sensitized luminescence and chemiluminescence properties of fluoroquinolone with different structure

    Science.gov (United States)

    Sun, Chunyan; Ping, Hong; Zhang, Minwei; Li, Hongkun; Guan, Fengrui

    2011-11-01

    Lanthanide sensitized luminescence and chemiluminescence (CL) are of great importance because of the unique spectral properties, such as long lifetime, large Stokes shifts, and narrow emission bands characteristic to lanthanide ions (Ln 3+). With the fluoroquinolone (FQ) compounds including enoxacin (ENX), norfloxacin (NFLX), lomefloxacin (LMFX), fleroxacin (FLRX), ofloxacin (OFLX), rufloxacin (RFX), gatifloxacin (GFLX) and sparfloxacin (SPFX), the luminescence and CL properties of Tb 3+-FQ and Eu 3+-FQ complexes have been investigated in this contribution. Ce 4+-SO 32- in acidic conditions was taken as the CL system and sensitized CL intensities of Tb 3+-FQ and Eu 3+-FQ complexes were determined by flow-injection analysis. The luminescence and CL spectra of Tb 3+-FQ complexes show characteristic peaks of Tb 3+ at 490 nm, 545 nm, 585 nm and 620 nm. Complexes of Tb 3+-ENX, Tb 3+-NFLX, Tb 3+-LMFX and Tb 3+-FLRX display relatively strong emission intensity compared with Tb 3+-OFLX, Tb 3+-RFX, Tb 3+-GFLX and Tb 3+-SPFX. Quite weak peaks with unique characters of Eu 3+ at 590 nm and 617 nm appear in the luminescence and CL spectra of Eu 3+-ENX, but no notable sensitized luminescence and CL of Eu 3+ could be observed when Eu 3+ is added into other FQ. The distinct differences on emission intensity of Tb 3+-FQ and Eu 3+-FQ might originate from the different energy gap between the triplet levels of FQ and the excited levels of the Ln 3+. The different sensitized luminescence and CL signals among Tb 3+-FQ complexes could be attributed to different optical properties and substituents of these FQ compounds. The detailed mechanism involved in the luminescence and CL properties of Tb 3+-FQ and Eu 3+-FQ complexes has been investigated by analyzing the luminescence and CL spectra, quantum yields, and theoretical calculation results.

  20. Study on the luminescence behavior of lanthanide ions with luminol by flow injection chemiluminescence analysis

    International Nuclear Information System (INIS)

    This work first described the linear quenching of LnIII (including LaIII, CeIII, PrIII, NdIII, SmIII, EuIII, GdIII, TbIII, DyIII, HoIII, ErIII, TmIII, YbIII and LuIII) on luminol–dissolved oxygen chemiluminescence system and possible quenching mechanism by flow injection analysis. The general equation of the decrement of CL intensity with LnIII concentration, ΔI=AlnCLn+B, was given and the interaction model of LnIII with luminol, lg[(I0−I)/I]=rlg[Ln]+lgK, was established. The sensitive factor A was found to be linear with the physical parameters (Z, γ±, Eo and ΔHhyd). By plotting A against Z, it could be seen light lanthanides (LL) and heavy lanthanides (HL) presented a good symmetry. The results showed that the reaction of LnIII with luminol was a spontaneous process by the electrostatic force with the association constants K at 106–107 level. The binding constants KD (about 104–105) and the number of binding sites n (about 1) of LnIII (LaIII, EuIII, GdIII, TbIII and LuIII) to bovine serum albumin were first given by FI–CL, and the binding ability of LnIII followed the pattern: LaIII

  1. A Chemiluminescent Protein Microarray Method for Determining the Seroglycoid Fucosylation Index.

    Science.gov (United States)

    Zhang, Aiying; Skog, Sven; Wang, Shengqi; Ke, Yang; Zhang, Yonghong; Li, Kang; He, Ellen; Li, Ning

    2016-01-01

    The Lens culinaris agglutinin-reactive fraction of AFP (AFP-L3) is widely used to screen for hepatocellular carcinoma (HCC) in Japan and China. We developed a chemiluminescent protein microarray for determining the AFP-L3/AFP index (the ratio of AFP-L3 to total AFP, AFP-L3%) by fixing AFP-specific antibodies and Lens culinaris lectin on aldehyde-coated glass slides. Serum samples were tested for AFP using an enzyme-linked immunosorbent assay (ELISA) to validate the microarray. AFP-L3 was detected using Hotgen Biotech glycosyl capture spin column pretreatment technology and ELISA. When the AFP cut-off value was set to 20 ng/ml, the protein microarray displayed 89.74% sensitivity and 100% specificity for HCC diagnosis, and the ELISA displayed 87.17% sensitivity and 100% specificity. When the AFP-L3% cut-off value was set to 0.1, the protein microarray displayed 56.41% sensitivity and 100% specificity for HCC diagnosis, and the ELISA displayed 53.84% sensitivity and 100% specificity. The ROC curve for the HCC diagnosis showed that the AFP area under the ROC curve (AUC = 0.996; 95% CI: 0.986-1.005) was much higher than that of AFP-L3 (AUC = 0.857; 95% CI: 0.769-0.94) and AFP-L3% (AUC = 0.827; CI: 0.730-0.924). The microarray assay used in this study is a highly sensitive, accurate, and efficient assay for the determination of the AFP-L3%. PMID:27528397

  2. Serum dosage of CPK-MB in dogs with ST deviation by chemiluminescence

    Directory of Open Access Journals (Sweden)

    André L.F. Santos

    2014-12-01

    Full Text Available Abstract: Although frequently in humans, hypoxic and ischemic heart diseases are poorly documented in dogs, with only few reports of acute myocardial infarction (AMI in this species. Some electrocardiographic findings might suggest myocardium hypoxia/ischemia, like ST segment elevation or depression, but there are no studies showing whether deviations in ST segment are associated to myocardial injury and serum increase of creatine phosphokinase (CPK-MB. In order to investigate possible myocardial cells injury in poor perfusion conditions, 38 dogs were studied, 20 with normal electrocardiogram and 18 with ST segment elevation or depression, recorded in lead II, at a paper speed of 50 mm/sec and N sensibility (1mV=1cm. Serum measurement of creatine phosphokinase isoenzyme MB (CPK-MB in normal dogs (group 1 determined control values (in ng/mL, which were compared to those obtained from dogs with deviation (group 2, which allowed confirmation or not of myocardial injury. CPK-MB mean values obtained from dogs in groups 1 and 2 were 0.540ng/ml (SD±0.890ng/mL and 0.440ng/mL (SD±1.106, respectively. At a significance level of 5%, the relation of CPK-MB with age, mass and total creatine phosphokinase (CPK-T was not significant in groups 1 and 2. CPK-MB showed no difference, at 5% level, between groups 1 and 2. In conclusion, it is possible to use the human chemiluminescent immunometric assay kit in canine species and that hypoxia/ischemia revealed by ST segment deviation does not mean significant myocardium injury.

  3. An assessment of non-linear dose response in chemiluminescence dosimetry

    Energy Technology Data Exchange (ETDEWEB)

    Jun, J.S. [Department of Physics, Chungnam National University, Taejon (Korea); Dalheimer, A.; Guggenberger, R. [Institute of Applied Radiation Protection, Federal Office of Radiation Protection, Oberschleissheim (Germany)

    2000-05-01

    Sugar and sorbite are well known to be convenient and suitable materials for chemiluminescence (CL) as well as electron spin resonance (ESR) dosimetry, in particular for retrospective dose assessment in an emergency and/or accident situation, mainly because of their close tissue-equivalence. In practice, however, the dose-CL response of those materials are not always shown to be satisfactorily linear. An attempt was made in this study to evaluate and correct the early supralinear dose-CL relationship appeared in the sugar and sorbite samples irradiated to gamma-ray doses of 0.5 to 10 Gy. In consideration of a similarity of the non-linear CL-dose relationship with the initial supralinearity shown in TL-dose response as depicted by Aitken, several recently proposed methods of expressing non-linear dose responses of TL, ESR or CL outputs were examined and investigated in order to look for a fittest means for the assessment of our supralinear dose-CL response of sugar and sorbite. As a result of an extensive study, merits and demerits of each method of representing non-linear dose response could be figured out in the light of our particular dose-CL relationship appeared in the sugar and sorbite samples. It is concluded that the 'supralinearity index', f(D), defined as a function of dose by Chen and McKeever is the most suitable function for expressing and correcting the non-linear dose-CL response shown in the initial low dose range of our irradiated sugar sorbite samples. The CL outputs corrected by use of f(D) are compared with those uncorrected, and the resultant CL sensitivities of the samples are numerically given together with re-evaluated dispersion of the sensitivities in terms of standard deviation. (author)

  4. Pharmacokinetic of pseudoephedrine in rat serum with luminol-pepsin chemiluminescence system by flow injection analysis.

    Science.gov (United States)

    Luo, Kai; Li, Yajuan; Zheng, Xiaohui; Song, Zhenghua

    2015-02-01

    Pepsin (Pep) accelerated the electron transferring rate of excited 3-aminophathlate and enhanced luminol-dissolved oxygen chemiluminescence (CL) intensity, and the flow injection (FI) luminol-Pep CL system was first developed. It was found that the CL intensity of luminol-Pep reaction could be remarkably inhibited by pseudoephedrine (PE); the decrement of CL intensity was linear to the logarithm of PE concentration in the range of 0.1∼100.0 nmol L(-1) with a detection limit of 0.03 nmol mL(-1) (3σ). At a flow rate of 2.0 mL min(-1), the complete process including washing and sampling was performed within 40 s, offering a sample throughput of 90 h(-1). This proposed method was successfully applied to determining PE in rat serum for 18 h after intragastric administration with the elimination ratio of 42.34 % and recoveries from 90.3 to 110.6 %. The pharmacokinetic results showed that PE could be rapidly absorbed into serum with peak concentration (C max) of 1.45 ± 0.18 g L(-1) at the time (T max) of 1.49 ± 0.02 h; the absorption half-life (0.35 ± 0.04 h), elimination half-life (1.86 ± 0.24 h), the area under curve (109.81 ± 6.03 mg L(-1) h(-1)), mean residence time (3.82 ± 0.27 h), and elimination rate constant (2.26 ± 0.23 L g(-1) h(-1)) in rats vivo were derived, respectively. The possible CL mechanism of luminol-Pep-PE reaction was discussed by FI-CL, fluorescence, and molecular docking (MD) methods.

  5. Magnetic Fe3O4 nanoparticle catalyzed chemiluminescence for detection of nitric oxide in living cells.

    Science.gov (United States)

    Wang, Huiliang; Li, Mei; Wang, Bing; Wang, Meng; Kurash, Ibrahim; Zhang, Xiangzhi; Feng, Weiyue

    2016-08-01

    Direct and real-time measurement of nitric oxide (NO) in biological media is very difficult due to its transient nature. Fe3O4 nanoparticles (nanoFe3O4) because of their unique catalytic activities have attracted much attention as catalysts in a variety of organic and inorganic reactions. In this work, we have developed a magnetic Fe3O4 nanoparticle-based rapid-capture system for real-time detection of cellular NO. The basic principle is that the nanoFe3O4 can catalyze the decomposition of H2O2 in the system to generate superoxide anion (O2 (·-)) and the O2 (·-) can serve as an effective NO(·) trapping agent yielding peroxynitrite oxide anion, ONOO(-). Then the concentration of NO in cells can be facilely determined via peroxynitrite-induced luminol chemiluminescence. The linear range of the method is from 10(-4) to 10(-8) mol/L, and the detection of limit (3σ, n = 11) is as low as 3.16 × 10(-9) mol/L. By using this method, the NO concentration in 0.1 and 0.5 mg/L LPS-stimulated BV2 cells was measured as 4.9 and 11.3 μM, respectively. Surface measurements by synchrotron X-ray photoelectron spectroscopy (SRXPS) and scanning transmission X-ray microscopy (STXM) demonstrate the catalytic mechanism of the nanoFe3O4-based system is that the significantly excess Fe(II) exists on the surface of nanoFe3O4 and mediates the rapid heterogeneous electron transfer, thus presenting a new Fe2O3 phase on the surface.

  6. Magnetic Fe3O4 nanoparticle catalyzed chemiluminescence for detection of nitric oxide in living cells.

    Science.gov (United States)

    Wang, Huiliang; Li, Mei; Wang, Bing; Wang, Meng; Kurash, Ibrahim; Zhang, Xiangzhi; Feng, Weiyue

    2016-08-01

    Direct and real-time measurement of nitric oxide (NO) in biological media is very difficult due to its transient nature. Fe3O4 nanoparticles (nanoFe3O4) because of their unique catalytic activities have attracted much attention as catalysts in a variety of organic and inorganic reactions. In this work, we have developed a magnetic Fe3O4 nanoparticle-based rapid-capture system for real-time detection of cellular NO. The basic principle is that the nanoFe3O4 can catalyze the decomposition of H2O2 in the system to generate superoxide anion (O2 (·-)) and the O2 (·-) can serve as an effective NO(·) trapping agent yielding peroxynitrite oxide anion, ONOO(-). Then the concentration of NO in cells can be facilely determined via peroxynitrite-induced luminol chemiluminescence. The linear range of the method is from 10(-4) to 10(-8) mol/L, and the detection of limit (3σ, n = 11) is as low as 3.16 × 10(-9) mol/L. By using this method, the NO concentration in 0.1 and 0.5 mg/L LPS-stimulated BV2 cells was measured as 4.9 and 11.3 μM, respectively. Surface measurements by synchrotron X-ray photoelectron spectroscopy (SRXPS) and scanning transmission X-ray microscopy (STXM) demonstrate the catalytic mechanism of the nanoFe3O4-based system is that the significantly excess Fe(II) exists on the surface of nanoFe3O4 and mediates the rapid heterogeneous electron transfer, thus presenting a new Fe2O3 phase on the surface. PMID:27289465

  7. Characterization of quinone derived protein adducts and their selective identification using redox cycling based chemiluminescence assay.

    Science.gov (United States)

    Elgawish, Mohamed Saleh; Kishikawa, Naoya; Ohyama, Kaname; Kuroda, Naotaka

    2015-07-17

    The cytotoxic mechanism of many quinones has been correlated to covalent modification of cellular proteins. However, the identification of relevant proteins targets is essential but challenging goals. To better understand the quinones cytotoxic mechanism, human serum albumin (HSA) was incubated in vitro with different concentration of menadione (MQ). In this respect, the initial nucleophilic addition of proteins to quinone converts the conjugates to redox-cycling quinoproteins with altered conformation and secondary structure and extended life span than the short lived, free quinones. The conjugation of MQ with nucleophilic sites likewise, free cysteine as well as ɛ-amino group of lysine residue of HSA has been found to be in concentration dependent manner. The conventional methods for modified proteins identification in complex mixtures are complicated and time consuming. Herein, we describe a highly selective, sensitive, simple, and fast strategy for quinoproteins identification. The suggested strategy exploited the unique redox-cycling capability of quinoproteins in presence of a reductant, dithiothreitol (DTT), to generate reactive oxygen species (ROS) that gave sufficient chemiluminescence (CL) when mixed with luminol. The CL approach is highly selective and sensitive to detect the quinoproteins in ten-fold molar excess of native proteins without adduct enrichment. The approach was also coupled with gel filtration chromatography (GFC) and used to identify adducts in complex mixture of proteins in vitro as well as in rat plasma after MQ administration. Albumin was identified as the main protein in human and rat plasma forming adduct with MQ. Overall, the identification of quinoproteins will encourage further studies of toxicological impact of quinones on human health. PMID:26044383

  8. Multiplexed detection of two proteins by a reaction kinetics-resolved chemiluminescence immunoassay strategy.

    Science.gov (United States)

    Wang, Wenwen; Ouyang, Hui; Yang, Shijia; Wang, Lin; Fu, Zhifeng

    2015-02-21

    A multiplexed immunoassay method was proposed for the sequential detection of two proteins in a single run based on a novel chemiluminescence (CL) reaction kinetics-resolved strategy. This method was established using acridinium ester (AE) and alkaline phosphatase (ALP) as the signal probes due to the significant difference in their CL reaction kinetics characteristics. Mouse IgG (MIgG) and mouse IgM (MIgM) were detected as the model analytes with a competitive immunoassay format. AE and ALP were used to tag goat anti-mouse IgG and rabbit anti-mouse IgM, respectively, to form two immunocomplexes. The two CL reactions with flash type and glow type kinetics characteristics were triggered simultaneously by adding the coreactants, then the CL signals from the two reactions were recorded after 0.2 s and 500 s of the reaction triggering, respectively. The multiplexed CL immunoassay provided a wide range of 0.50-200 ng mL(-1), with a low detection limit of 0.16 ng mL(-1) (S/N = 3) for both MIgG and MIgM. Additionally, no obvious signal overlap was observed in the multiplexed immunoassay. The proposed method was successfully applied for the detection of MIgG and MIgM levels in mouse serums, and the results were in good agreement with those from the reference ELISA method. We anticipate that it can be used in some other areas such as drug screening, food safety, environment monitoring and clinical diagnosis. PMID:25531210

  9. Evaluation of a new serological test for syphilis based on chemiluminescence assay in a tertiary care hospital

    Directory of Open Access Journals (Sweden)

    Aseem K Tiwari

    2015-01-01

    Full Text Available Context: Syphilis is a transfusion transmissible infections and it is mandatory to do serological test for syphilis (STS on all donor blood samples. STS is usually based on detection of antibodies against the cardiolipin-lecithin antigen or against the Treponema-specific antigen. STS with good sensitivity and specificity helps enhance blood safety and consolidation of STS along with other transfusion transmittable infections such as human immunodeficiency virus, hepatitis-C virus, and hepatitis-B virus helps in reducing the errors and enhances efficiency. Aims: This study was designed to evaluate the performance of newly introduced VITROS ® syphilis Treponema pallidum agglutination (TPA assay based on enhanced chemiluminescence principle for its analytical performance for use as a STS on donor blood samples at a tertiary care health center in National Capital Region, India. Materials and Methods: A total of 108 random blood units collected from the donors (both voluntary and replacement donors and 28 known syphilis sero-reactive samples stored at −20°C, were used to evaluate the performance of VITROS ® syphilis TPA assay based on enhanced chemiluminescence assay on VITROS ® ECiQ immunodiagnostics system along with its analytical performance in terms of its sensitivity, precision, cross-reactivity and interference studies. Results: VITROS ® syphilis TPA showed 100% sensitivity and specificity with precision (20 days study of <10% co-efficient of variation. There was no cross-reactivity with other viral and auto-immune antibodies. No interference was observed from endogenous interfering substances like free hemoglobin or fats. Conclusions: Performance of the VITROS ® syphilis TPA assay meets the requirements for its use as STS in blood bank, thus allowing consolidation with other transfusion transmittable infections screening assay on chemiluminescence platform, which is highly valuable for optimizing workflow and efficiency.

  10. 化学发光法检测肿瘤标志物%Tumor Markers Detection by Chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    贺艳峰; 张家明; 郭小英; 朱广华; 陆祖宏

    2005-01-01

      肿瘤标志物作为诊断肿瘤的一项重要指标,其检测对于疾病的治疗与预后具有重要的意义。化学发光法是一种灵敏度非常高的分析方法,现已广泛应用于检测肿瘤标志物的研究中。随着发光试剂与检测仪器的不断进步,化学发光法研究也在不断深入发展,作为检测肿瘤标志物的一种重要方法,将为肿瘤的早期诊断以及愈后的监测发挥更重要的作用。本文从化学发光体系、检测仪器以及高新技术在该领域的应用及产生的影响三个角度,综述了化学发光法用于肿瘤标志物检测的研究进展,对该领域未来的发展进行了展望。%  Tumor markers are much important signals in patients’ diagnosis and prognosis of tumors. Chemiluminescence is an analytical method of high sensitivity. Nowadays it has been widely used in detecting tumor markers. With the development of chemiluminescent reagents and detectors, it will be an important method of detecting tumor markers, large influences for earlier diagnosis tumors and be a very important method in clinical laboratory. The paper summarized the development of chemiluminescence substrate systems, the detecting instruments, the application and influence of the high technology in this field, and the future prospect as well.

  11. Microplate chemiluminescence enzyme immunoassay for the quantitative evaluation of carbohydrate antigen 72-4 in human serum

    Institute of Scientific and Technical Information of China (English)

    JIN Hui; WANG Xu; XIN TianBing; GAO Peng; LIN JinMing; LIANG ShuXuan

    2008-01-01

    A highly sensitive and specific microplate chemiluminescence enzyme immunoassay (CLEIA) was de-veloped for the quantitative evaluation of carbohydrate antigen 72-4 (CA72-4) in human serum, using luminol-H2O2 catalyzed by horseradish peroxidase (HRP) as the chemiluminescence system. The sim-ple and quick determination was accomplished through a sandwich reaction mode. Several physico-chemical parameters of the immunoreaction, including incubation conditions, antibody coating condi-tions, dilution ratio of anti-CA72-4-HRP conjugate, and chemiluminescence reaction time, were studied and optimized. The proposed method exhibited a linear range of 0-200 U/mL with correlation coeffi-cient and detection limit of 0.9995 and 0.18 U/mL, respectively. The inter-assay and intra-assay coeffi-cients of variation (CV) were both less than 10%. The average recovery of two clinical sera with low and high concentration CA72-4 was 99.3% and 98.7%, respectively. Normal tumor markers, including AFP, CEA, CA2.4-2, CA19-9 and CA15-3, did not cross-react with each other. The method's stability was evaluated by assessing its analytical performance after storing the immunoreagents at 4℃ and 37℃ for 7 days. Little difference was found, indicating satisfactory stability of the method. The present method has been successfully applied to the detection of CA72-4 human serum, and showed a good correlation with the commercially available ELISA kit (r2=0.9383). This method showed great potential in the fabrication of diagnostic kit for CA72-4, and could be well used in diagnosis of cancer in clinical practice.

  12. Peroxide-dependent amino acid oxidation and chemiluminescence catalysed by magnesium-pyridoxal phosphate-glutamate complex.

    Science.gov (United States)

    Meyer, B U; Schneider, W; Elstner, E F

    1992-08-01

    Magnesium-pyridoxal-5'-phosphate-glutamate (MPPG) has been shown to ameliorate atherosclerotic symptoms in rabbits. In vitro, MPPG in the presence of peroxides such as cholesterolhydroperoxide or cumene hydroperoxide and Mn2+ ions produces "excited states" measurable as chemiluminescence or ethylene release from 1-aminocyclopropane-1-carboxylic acid (ACC). The reactions are stimulated synergistically by unsaturated fatty acids. Pyridoxal phosphate exhibits similar properties, but can be differentiated from the activities of MPPG or the sum of the components present in MPPG. PMID:1510700

  13. Electrogenerated chemiluminescence of luminol using low-cost electrodes; Quimioluminiscencia electrogenerada del luminol usando electrodos de bajo costo

    Energy Technology Data Exchange (ETDEWEB)

    Salgado, Guillermo; Navarrete, Jose [Universidad Finis Terrae, Santiago (Chile). Facultad de Educacion]. E-mail: salgado@interaccess.cl; Bustos, Carlos; Sanchez, Cristian; Ugarte, Ricardo [Universidad Austral de Chile, Valdivia (Chile). Inst. de Quimica

    2006-03-15

    purpose of the study was to observe eletrogenerated chemiluminescence (ECL) of luminol using different materials as electrodes such as wires, nails, coins, razor blades, etc. Based on the experimental observations and the mechanisms proposed in the literature, students should be capable of analyzing their results in order to understand the phenomena studied. Students could then elaborate a mechanism of action consistent with the experimental results which could rationalize the formation of intermediates in the reaction and the dependence of ECL on solution pH in addition to other points of interest. (author)

  14. Clinical Evaluation of the ZstatFlu-II Test: a Chemiluminescent Rapid Diagnostic Test for Influenza Virus

    OpenAIRE

    Hamilton, Marilyn S.; Abel, David M.; Ballam, Yolanda J.; Otto, Mary K.; Nickell, Angela F.; Pence, Lisa M.; Appleman, James R.; Shimasaki, Craig D.; Achyuthan, Komandoor E.

    2002-01-01

    Exploiting the high sensitivity of the chemiluminescence phenomenon, an accurate and sensitive point-of-care test, called the ZstatFlu-II test (ZymeTx, Inc., Oklahoma City, Okla.), was developed to detect influenza virus infections. The ZstatFlu-II test takes 20 min and requires approximately 2 min of “hands-on” time for operational steps. The ZstatFlu-II test does not distinguish between infections with influenza virus types A and B. ZstatFlu-II test results are printed on Polaroid High-Spee...

  15. A New Chemiluminescent Method for Evaluation of the Functional Activity of Neutrophils in Patients with Type 2 Diabetes Mellitus.

    Science.gov (United States)

    Proskurnina, E V; Sozarukova, M M; Polimova, A M; Prudnikova, M A; Ametov, A S; Vladimirov, Yu A

    2016-06-01

    Functional activity of neutrophils was evaluated by the chemiluminescent method with successive double stimulation by soluble stimuli with different mechanisms of action: phorbol-12-myristate-13-acetate (PMA) and phormyl-methionyl-leucyl-phenilalanine (fMLP). The study was carried out in 26 patients receiving oral sugar-reducing therapy. In addition to the functional activity of neutrophils, the levels of TBA reactive products, inflammation markers, blood clotting values, and biochemical parameters were measured. The results showed mainly reduction of the granulocytic component of the immune system in the patients. PMID:27388632

  16. Emission spectroscopic properties of water soluble porphyrins in hydrogen peroxide chemiluminescence system with d- and f-electron metals

    Science.gov (United States)

    Staninski, Krzysztof; Kaczmarek, Małgorzata; Lis, Stefan; Elbanowski, Marian

    2003-02-01

    Two water-soluble porphyrins: 4,4',4'',4'''-(porphine-5,10,15,20-tetrayl)-tetrakis (benzoic acid) (TCPPH 2) and 4,4',4'',4'''-(porphine-5,10,15,20-tetrayl)-tetrakis (benzenesulfonic acid) (TSPPH 2) have been subjected to spectroscopic study in the presence of d-electron metals: Zn(II) and Cu(II) and f-electron metals: La(III), Eu(III), Gd (III) and Yb(III). Results of the spectrophotometric study have provided evidence proving the complexation of Cu(II) and Zn(II) cations by porphine in water solutions and the complexation of lanthanide ions exclusively by peripheral carboxyl and sulfonic groups. For the first time, chemiluminescence of the systems containing porphyrins has been measured without the use of strongly luminescent reagents such as TCPO or luminol. The emission spectra of the systems porphyrin/metal ion/H 2O 2 have been recorded and the quantum yield of their luminescence has been measured. The absorption spectra of the systems recorded before and after the reaction in the presence of hydrogen peroxide are identical, which means that the porphyrin ring does not undergo destruction. A significant similarity between the fluorescence and chemiluminescence spectra indicates a possibility of excitation of porphyrins and their complexes in the reaction with hydrogen peroxide.

  17. A sensitive chemiluminescent enzyme immunoassay for the bioanalysis of carboxyl-terminal B-chain analogues of human insulin.

    Science.gov (United States)

    Cao, Y; Smith, W C; Bowsher, R R

    2001-08-01

    Quantification of analogues of human insulin in biological matrices is complicated by differences in their immunoreactivity and the presence of both the analogue and endogenous concentrations of insulin in test samples. To facilitate pharmacokinetic comparisons of carboxyl-terminal B-chain analogues of human insulin, we undertook development of a sensitive ELISA. The ELISA detection method was optimized systematically to permit routine analysis of 10-microl serum samples. Accordingly, a noncompetitive 'sandwich' chemiluminescent ELISA was validated for the quantification of carboxyl-terminal B-chain insulin analogues in human serum over a concentration range from 5 to 3125 pM. The mean bias (RE%) within the validated range varied from -10.3 to 4.3%, with an intermediate precision (inter-assay CV%) from 4.2 to 11.5%. The two-sided 90% expectation tolerance interval for total measurement error was within +/-25% of the nominal concentration for all levels of validation samples. Insulin lispro, human insulin, proinsulin, despentapeptide insulin (DPI) and porcine insulin displayed comparable crossreactivity in the ELISA. Potential utility of the new assay for insulin bioanalysis in nonhuman species was investigated by assessing the pharmacokinetic profile of DPI in rats following administration of a single subcutaneous dose. The sensitive chemiluminescent detection method is simple to perform and should be readily adaptable for ELISAs of other therapeutic proteins.

  18. Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination

    Directory of Open Access Journals (Sweden)

    Muhammad Asgher

    2014-01-01

    Full Text Available Rose Bengal photosensitized flow injection chemiluminescence method is reported using luminol-Cu(II for the determination of vitamins A and C in pharmaceutical formulations. The reaction is based on the enhancement effect of analyte in the production of anion radicals of Rose Bengal (RB•− which rapidly interact with dissolved oxygen and generate superoxide anions radicals (O2•− and hydrogen peroxide (H2O2. Highly reactive hydroxyl radicals (•OH were produced via dismutation of H2O2 by catalyst (Cu2+. The generated superoxide anions radicals and hydroxyl radicals thus oxidize luminol in alkaline medium to generate strong chemiluminescence. The limit of detection (3s of the blank, n=6 of vitamins A and C and RB was found to be 0.008, 0.005, and 0.05 μg mL−1, respectively. The sample throughput of 70 h−1 for vitamins A and C and 30 h−1 for RB was found. Calibration curve was linear in the range of 0.05–15, 0.01–20, and 0.1–50 μg mL−1 for vitamins A and C and RB, respectively, with relative standard deviations (RSDs; n=3 in the range 1.6–3.6%. The method was successfully applied to pharmaceutical formulations and the results obtained were in good agreement with the labeled values.

  19. Rapid and Sensitive Detection of RNA Viruses Based on Reverse Transcription Loop-Mediated Isothermal Amplification, Magnetic Nanoparticles, and Chemiluminescence.

    Science.gov (United States)

    Wang, Jiuhai; Lu, Peng; Yan, Jieni; Zhang, Yufan; Huang, Lanye; Ali, Zeeshan; Li, Zhiyang; He, Nongyue

    2016-04-01

    RNA viruses, particularly, the highly pathogenic avian influenza (HPAI) virus, pose serious health concerns, and cause huge economic losses worldwide. Diagnostic tools for the early detection of these deadly RNA viruses are urgently needed to implement treatment and disease control strategies. Conventional reverse transcription polymerase chain reaction (RT-PCR)-based chemiluminescent (RT-PCR-CL) detection is frequently used for the diagnosis of viral infections. However, the requirements for expensive PCR machines and longer thermocycling times are significant drawbacks. In this study, we propose a method based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) combined with chemiluminescence (CL) to detect H7N9 virus. The proposed method does not require any expensive instruments, and processing time is remarkably shortened compared to that of RT-PCR-CL. Since several factors including RT-LAMP temperature, probe concentration, hybridization temperature, and hybridization duration might affect the CL signal, each of these parameters was investigated and optimized. One thousand copies/mL of H7N9 RNA were detectable using the optimized RT-LAMP-CL method. The detection time was significantly reduced by using RT-LAMP, in comparison with conventional RT-PCR-CL. This technique holds great promise for viral detection and diagnosis, especially with regard to avian influenza virus. PMID:27301197

  20. Chemiluminescent method for detection of eutrophication sources by estimation of organic amino nitrogen and ammonium in water.

    Science.gov (United States)

    Meseguer-Lloret, S; Molins-Legua, C; Verdú-Andrés, J; Campíns-Falcó, P

    2006-11-01

    An automatic method has been developed for the estimation of organic amino nitrogen (CH2-NH) and ammonium in water samples. We propose a continuous flow system in which nitrogen compounds react with hypochlorite reagent to produce chloramines. Subsequently, the mixture is mixed with luminol, generating a chemiluminescence signal. The signal emission at 425 nm, registered as a function of time, decreases as nitrogen concentration increases, due to the decrease on hypochlorite concentration. A large number of nitrogen compounds have been assayed and their sensitivities compared, in milligrams per liter nitrogen. The ammonium calibration graph, expressed as N, can be used for most of the assayed compounds. The linear interval was 0.24-4 mg L(-1) N, with the detection limit 0.07 mg L(-1) N. The chemiluminescence method was applied to the analysis of several kinds of real water samples, natural, lake, irrigation ditch, fountain, residual, and seawater in order to detect possible sources of eutrophication. The accuracy (% relative error) and precision were satisfactory, with mean values of 5 +/- 4 and 3 +/- 2, respectively. This procedure has been used to estimate nitrogen content in samples before and after Kjeldahl treatment. In the same samples, the N found for the untreated samples provided a good estimation of the N Kjeldahl. Sixty samples per hour can be analyzed, and the procedure can also be used for in situ monitoring.

  1. Suitability of thermoluminescence, chemiluminescence, ESR and viscosity measurements as detection method for the irradiation of medicinal herbs

    International Nuclear Information System (INIS)

    Chemiluminescence, electron spin resonance, thermoluminescence and viscosity measurements have been investigated for their suitability as detection method for the irradiation of the medicinal herbs anise seeds (anisi fructus), valerian roots (valerianae radix), redberry leaves (uvae ursi folium), birch leaves (betulae folium), greek hay seeds (foenugraeci semen), cayenne pepper (capsici fructus acer), black-aldertee bark (frangulae cortex), fennel fruits (feoniculi fructus), rose hip shells (cynosbati fructus), coltsfoot (farfarae folium), acorus roots (calami rhizoma), chamomile flowers (matricariae flos), caraway (carvi fructus), lavender flowers (lavandulae flos), linseed (lini semen), lime tree flowers (tiliae flos), St. Mary's thistle fruit (cardui mariae herba), lemon balm (melissae folium), java tea (orthosiphonis folium), peppermint (menthae piperitae folium), sage leaves (salviae folium), scouring rush (equiseti herba), senna leaves (sennae folium), plantain herbs (plantaginis lanceolata herba), thyme herbs (thymi herba), juniper berries (juniperi fructus), hawthorne herbs (crataegi folium), wheat starch (amylum tritici) and wormwood (absinthii herba). Depending on the herbs, the methods used were more or less suitable. Chemiluminescence measurements showed the smallest differences between untreated and irradiated samples whereas thermoluminescence measurements on isolated minerals from the vegetable drugs gave better results. In some herbs radiation-specific radicals could be identified by ESR spectroscopy. Viscosity measurement is suitable for some herbs as fast and inexpensive method for screening. (orig.)

  2. PIV, high-speed PLIF and chemiluminescence imaging for near-spark-plug investigations in IC engines

    International Nuclear Information System (INIS)

    Measurements of the local flow and mixture condition near the spark plug of internal combustion engines are important to characterize their influence on ignition and combustion performance. This is especially true for direct-injection engines where limited time is available for mixture formation and optimum stratification of the fuel/air mixture to achieve best performance. Transient processes need to be visualized in an optically challenging environment. The application of digital Particle Image Velocimetry (PIV) for flow field measurements along with crank angle-resolved planar laser induced fluorescence (PLIF) and chemiluminescence imaging is discussed in the context of investigations of a highly stratified sprayguided direct-injection engine. Flow fields were captured in a firing optical single-cylinder engine to study the interaction of the fast spray and the underlying in-cylinder tumble flow. The impingement of the fuel spray on the spark plug electrodes and subsequent dispersion of the fuel cloud was filmed at a rate of 12kHz with a new PLIF technique using a diode-pumped Nd:YAG laser. Subsequent flame development and combustion progress could be followed via high-speed imaging of OH* chemiluminescence. This approach was also combined with double- pulse PLIF imaging of fuel distributions

  3. Chemiluminescence enzyme immunoassay based on magnetic nanoparticles for detection of hepatoceUular carcinoma marker glypican-3

    Institute of Scientific and Technical Information of China (English)

    Qian-Yun Zhang; Hui Chen; Zhen Lin; Jin-Ming Lin

    2011-01-01

    Glypican-3 (GPC3) is reported as a great promising tumor marker for hepatocellular carcinoma (HCC) diagnosis. Highly sensitive and accurate analysis of serum GPC3 (sGPC3), in combination with or instead of traditional HCC marker alpha-fetoprotein (AFP), is essential for early diagnosis of I-ICC. Biomaterial-functionalized magnetic particles have been utilized as solid supports with good biological compatibility for sensitive immunoassay. Here, the magnetic nanoparticles (MnPs) and magnetic microparticles (MmPs) with carboxyl groups were further modified with streptavidin, and applied for the development of chemiluminescence enzyme immunoassay (CLEIA). After comparing between MnPs- and MmPs-based CLEIA, MnPs-based CLEIA was proved to be a better method with less assay time, greater sensitivity, better linearity and longer chemiluminescence platform. MnPs-based CLEIA was applied for detection of sGPC3 in normal liver, hepatocirrhosis, secondary liver cancer and HCC serum samples. The results indicated that sGPC3 was effective in diagnosis of HCC with high performance.

  4. HNO3 analyzer by scrubber difference and the NO-ozone chemiluminescence method

    International Nuclear Information System (INIS)

    A fast response analyzer for HNO3 in highly polluted air is described. The time resolution attainable was 12s. The method is based on the difference in a technique for HNO3-scrubbed and non-scrubbed air and the reduction of HNO3 to NO with the use of a line of catalytic converters and a method for the subsequent NO-ozone chemiluminescence. A sample air stream, in which particulates are removed with a Teflon filter, is divided into two channels. CH-1 is directly connected to the converter line, and CH-2 contains a HNO3 scrubber packed with a nylon fiber that goes to another converter line. Each converter line is composed of a hot quartz-bead converter (QBC) and a molybdenum converter (MC) in a series. A QBC reduces HNO3 to (NO+NO2), which is called NOx. The MC reduces the NOx to NO. For CH-1, the analyzer detects most compounds that typically comprise NOy (J. Geophys. Res. 91(1986) 9781). These CH-1 compounds are called NOy' hereafter (NOy-particulate nitrate) because the particulates are removed by the filter. A difference in the detector signal for the two channels indicates HNO3. For a blank test, atmospheric air in which HNO3 was pre-scrubbed by an extra nylon fiber was introduced to the analyzer. Variations in the blank value were 0.38 ± 0.42 and 0.34 ± 0.55ppb during the high readings (NOy'-HNO3) (called NOy* hereafter) (111 ± 12ppb, N=180), and low NOy* readings (62 ± 8ppb, N=180), respectively, indicating that the lowest detection limit of the analyzer is 1.1ppb (2 σ). When the data obtained with the analyzer is compared to the data using the denuder method, a linear correlation with the regression of Y=0.973X+0.077(r2=0.916 (N=20)) in the range of 0-6.5ppb HNO3 is obtained, which is an excellent agreement. Atmospheric monitoring was carried out at Kobe. Although the average concentration of HNO3 was 2.6±l.3ppb, ca.10ppb for a HNO3 concentration was occasionally observed when the NOy* concentration was high, i.e., more than 100ppb. (author)

  5. HNO 3 analyzer by scrubber difference and the NO-ozone chemiluminescence method

    Science.gov (United States)

    Yamamoto, Masatoshi; Tamaki, Motonori; Bandow, Hiroshi; Maeda, Yasuaki

    A fast response analyzer for HNO 3 in highly polluted air is described. The time resolution attainable was 12 s. The method is based on the difference in a technique for HNO 3-scrubbed and non-scrubbed air and the reduction of HNO 3 to NO with the use of a line of catalytic converters and a method for the subsequent NO-ozone chemiluminescence. A sample air stream, in which particulates are removed with a Teflon filter, is divided into two channels. CH-1 is directly connected to the converter line, and CH-2 contains a HNO 3 scrubber packed with a nylon fiber that goes to another converter line. Each converter line is composed of a hot quartz-bead converter (QBC) and a molybdenum converter (MC) in a series. A QBC reduces HNO 3 to (NO+NO 2), which is called NO x. The MC reduces the NO x to NO. For CH-1, the analyzer detects most compounds that typically comprise NO y (J. Geophys. Res. 91 (1986) 9781). These CH-1 compounds are called NO y' hereafter (NO y-particulate nitrate) because the particulates are removed by the filter. A difference in the detector signal for the two channels indicates HNO 3. For a blank test, atmospheric air in which HNO 3 was pre-scrubbed by an extra nylon fiber was introduced to the analyzer. Variations in the blank value were 0.38±0.42 and 0.34±0.55 ppb during the high readings (NO y'-HNO 3 ) (called NO y* hereafter) (111±12 ppb, N=180), and low NO y* readings (62±8 ppb, N=180), respectively, indicating that the lowest detection limit of the analyzer is 1.1 ppb (2 σ). When the data obtained with the analyzer is compared to the data using the denuder method, a linear correlation with the regression of Y=0.973 X+0.077 ( r2=0.916 ( N=20)) in the range of 0-6.5 ppb HNO 3 is obtained, which is an excellent agreement. Atmospheric monitoring was carried out at Kobe. Although the average concentration of HNO 3 was 2.6±1.3 ppb, ca.10 ppb for a HNO 3 concentration was occasionally observed when the NO y* concentration was high, i.e., more than

  6. Study on the luminescence behavior of lanthanide ions with luminol by flow injection chemiluminescence analysis

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Xiaoqing [College of Chemistry and Material Science, Northwest University, 710069 Xi' an (China); Wang, Zhuming [College of Earth Sciences and Land Resources, Chang' an University, 710054 Xi' an (China); Li, Yajuan [College of Chemistry and Material Science, Northwest University, 710069 Xi' an (China); Zhang, Yun [Department of pharmacy, Xi' an Medical University, 710021 Xi' an (China); Guo, Jie; Zhao, Jingchan [College of Chemistry and Material Science, Northwest University, 710069 Xi' an (China); Song, Zhenghua, E-mail: songzhenghua@hotmail.com [College of Chemistry and Material Science, Northwest University, 710069 Xi' an (China)

    2015-06-15

    This work first described the linear quenching of Ln{sup III} (including La{sup III}, Ce{sup III}, Pr{sup III}, Nd{sup III}, Sm{sup III}, Eu{sup III}, Gd{sup III}, Tb{sup III}, Dy{sup III}, Ho{sup III}, Er{sup III}, Tm{sup III}, Yb{sup III} and Lu{sup III}) on luminol–dissolved oxygen chemiluminescence system and possible quenching mechanism by flow injection analysis. The general equation of the decrement of CL intensity with Ln{sup III} concentration, ΔI=AlnC{sub Ln}+B, was given and the interaction model of Ln{sup III} with luminol, lg[(I{sub 0}−I)/I]=rlg[Ln]+lgK, was established. The sensitive factor A was found to be linear with the physical parameters (Z, γ{sub ±}, E{sup o} and ΔH{sub hyd}). By plotting A against Z, it could be seen light lanthanides (LL) and heavy lanthanides (HL) presented a good symmetry. The results showed that the reaction of Ln{sup III} with luminol was a spontaneous process by the electrostatic force with the association constants K at 10{sup 6}–10{sup 7} level. The binding constants K{sub D} (about 10{sup 4}–10{sup 5}) and the number of binding sites n (about 1) of Ln{sup III} (La{sup III}, Eu{sup III}, Gd{sup III}, Tb{sup III} and Lu{sup III}) to bovine serum albumin were first given by FI–CL, and the binding ability of Ln{sup III} followed the pattern: La{sup III}

  7. Electrogenerated chemiluminescence of a cationic cyclometalated iridium complex–Nafion modified electrode in neutral aqueous solution

    International Nuclear Information System (INIS)

    Electrogenerated chemiluminescence (ECL) of a cationic cyclometalated iridium complex, [(pqcm)2Ir(bpy)](PF6) (1, pqcmH=2-phenyl-quinoline-4-carboxylic acid methyl ester, bpy=2,2′-bipyridine), was investigated at a bare glassy carbon electrode in CH3CN solution and 4 ECL peaks were observed. Then, the ECL of the iridium complex was studied in neutral phosphate buffer solution (PBS) by immobilizing it on a glassy carbon electrode. Two closely located ECL peaks were obtained at 1.07 and 1.40 V when the potential was scanned from −3.00 V to 2.20 V, while only one broad ECL peak located around −2.0 V was obtained when the potential was scanned from 2.20 V to −3.00 V. In the presence of oxalate, one ECL peak located around 1.22 V could be obtained except the broad ECL peak located at −2.00 V. The ECL peak at positive potential range was enhanced more than one magnitude in the presence of Nafion and was nearly 5-times higher than that of Ru(bpy)32+–Nafion modified electrode, suggesting that the synthesized iridium complex has great application potential in ECL detection. The ECL spectra of iridium complex were identical to its photoluminescence spectrum, indicating the same metal-to-ligand charge transfer (MLCT) excited states. The mechanisms of ECL were proposed based on the experimental results. The present ECL sensor gave a linear response for the oxalate concentration from 1.0×10−6 to 1.0×10−4 mol L−1 with a detection limit (S/N=3) of 9.1×10−7 mol L−1. -- Graphical abstract: Electrochemiluminescence (ECL) of immobilized novel cationic cyclometalated iridium complex in neutral phosphate buffer solution is reported for the first time. The intensity of iridium complex ECL is 5-times higher than that of Ru(bpy)32+ ECL. Highlights: ► Cationic cyclometalated iridium complex was modified on a bare electrode. ► Electrochemiluminescence (ECL) of the modified electrode was studied. ► The ECL intensity is higher than that of Ru(bpy)32+ modified

  8. Measurements in international units of antibody to hepatitis B surface antigen(anti-HBs) after immunization with a yeast-derived, subtype adr hepatitis B vaccine are considerably different between chemiluminescent immunoassay (CLIA) and chemiluminescent enzyme immunoassay (CLEIA).

    Science.gov (United States)

    Ogata, Norio

    2006-04-01

    The worldwide consensus of the minimum protective anti-HBs level against HBV infection is 10 mIU/mL on assays standardized by the World Health Organization (WHO) reference preparations. To investigate whether this value could be applied to recipients of yeast-derived recombinant HB vaccine containing the major surface protein of subtype adr (Bimmugen, Astellas Pharmaceutical, Tokyo), we compared anti-HBs measurements between chemiluminescent immunoassay (CLIA) (Architect Ausab, Abbott Japan, Tokyo) and chemiluminescent enzyme immunoassay (CLEIA) (Lumipulse Forte, Fujirebio, Tokyo) in given serum samples obtained from the vaccinees. The vaccine and the two assay methods are currently in a wide use in Japan. The study included 300 medical students who completed a standard vaccination course (0, 1 and 6 months). Serum samples obtained 1 month or 13 months after completing the vaccination were simultaneously tested for anti-HBs by CLIA and CLEIA. In 147 samples with quantifiable values on both CLIA and CLEIA (10 to 1000 mIU/mL) the geometric mean titer on CLEIA (225.0 mIU/mL) was significantly higher than that on CLIA (94.5 mIU/mL) (p < 0.0001). Of 26 subjects with CLIA measurements below 10 mIU/mL, 15 samples (57.7%) showed CLEIA measurements more than 10 mIU/mL. Thus, in the subtype adr-vaccinees CLEIA demonstrated considerably high serum anti-HBs measurements compared to CLIA and discordance in determining critical anti-HBs level of 10 mIU/mL was observed in more than half the samples. This suggests that the minimum HBV-protective anti HBs titer of 10 mIU/mL is difficult to be introduced to Japan where subtype adr-HB vaccines or -HBV infection are prevalent, unless characteristics of assay methods are carefully evaluated.

  9. Interference of oxygen, carbon dioxide, and water vapor on the analysis for oxides of nitrogen by chemiluminescence

    Science.gov (United States)

    Maahs, H. G.

    1975-01-01

    The interference of small concentrations (less than 4 percent by volume) of oxygen, carbon dioxide, and water vapor on the analysis for oxides of nitrogen by chemiluminescence was measured. The sample gas consisted primarily of nitrogen, with less than 100 parts per million concentration of nitric oxide, and with small concentrations of oxygen, carbon dioxide, and water vapor added. Results obtained under these conditions indicate that although oxygen does not measurably affect the analysis for nitric oxide, the presence of carbon dioxide and water vapor causes the indicated nitric oxide concentration to be too low. An interference factor - defined as the percentage change in indicated nitric oxide concentration (relative to the true nitric oxide concentration) divided by the percent interfering gas present - was determined for carbon dioxide to be -0.60 + or - 0.04 and for water vapor to be -2.1 + or - 0.3.

  10. Control of excitation and quenching in multi-colour electrogenerated chemiluminescence systems through choice of co-reactant.

    Science.gov (United States)

    Barbante, Gregory J; Kebede, Noah; Hindson, Christopher M; Doeven, Egan H; Zammit, Elizabeth M; Hanson, Graeme R; Hogan, Conor F; Francis, Paul S

    2014-10-20

    We demonstrate a new approach to manipulate the selective emission in mixed electrogenerated chemiluminescence (ECL) systems, where subtle changes in co-reactant properties are exploited to control the relative electron-transfer processes of excitation and quenching. Two closely related tertiary-amine co-reactants, tri-n-propylamine and N,N-diisopropylethylamine, generate remarkably different emission profiles: one provides distinct green and red ECL from [Ir(ppy)3] (ppy=2-phenylpyridinato-C2,N) and a [Ru(bpy)3](2+) (bpy=2,2'-bipyridine) derivative at different applied potentials, whereas the other generates both emissions simultaneously across a wide potential range. These phenomena can be rationalized through the relative exergonicities of electron-transfer quenching of the excited states, in conjunction with the change in concentration of the quenchers over the applied potential range. PMID:25204830

  11. Simultaneous detection of monoethanolamine, diethanolamine, and triethanolamine by HPLC with a chemiluminescence reaction and online derivatization to tertiary amine.

    Science.gov (United States)

    Niina, Nobumitsu; Kodamatani, Hitoshi; Uozumi, Kayoko; Kokufu, Yuki; Saito, Keiitsu; Yamazaki, Shigeo

    2005-05-01

    In this paper we propose a new postcolumn detection method for compounds having primary, secondary, and tertiary amine moieties. The primary and secondary amine are delivatized by a reaction with epichlorohydrin having an epoxy moiety in a reaction coil to yield a tertiary amine with subsequent chemiluminescence detection using [Ru(bpy)3]3+. The liner values of the calibration curves of monoethanolamine (MEA), diethanolamine (DEA) and triethanolamine (TEA) were 0.02 - 1.0 nmol (r2 = 0.9986), 0.02 - 0.5 nmol (r2 = 0.9993) and 0.1 - 1.0 nmol (r2 = 0.9482), respectively. Also, the detection limits (S/N = 3) of MEA, DEA and TEA were 30, 25 and 40 pmol, respectively. The amount of DEA and TEA in shaving cream (60 microg/20 microL) were found to be 0.3 nmol and 14 nmol, respectively, by the proposed method. PMID:15913135

  12. Flow injection determination of metoclopramide based on KMnO4-HCHO chemiluminescence in a micellar medium

    International Nuclear Information System (INIS)

    A novel flow injection chemiluminescence (CL) analysis method for the determination of metoclopramide in the presence of sodium dodecyl sulfonate (SDS) surfactant micelles is described. This method is based on the luminescent properties of the KMnO4-HCHO-metoclopramide in acid medium sensitized by SDS. The optimized experimental conditions were evaluated and the possible mechanism was discussed. The CL increase is linearly related to the concentration of metoclopramide in the range 0-80.0 μg/ml with a detection limit of 31.3 ng/mL (S/N=3).The relative standard deviation for 20.0 μg/ml samples was 2.6% (n=11). The proposed method has been successfully applied to the determination of metoclopramide in tablets.

  13. Flow—injection Chemiluminescence Sensor for the Determination of Gallic Acid by Immobilizing Luminol and Periodate on Anionexchange Resin

    Institute of Scientific and Technical Information of China (English)

    鞠Huang先; 张四纯; 周国俊

    2002-01-01

    A novel chemiluminescence (CL) sensor for the determination of gallic acld combined with folw injection analysis was developed by electrostatically immobilizing hminol and periodate on anion-exchange resins respectively.Gallic acid was sensed by its enhancing effect on the weak CL reaction between luminol and periodate,which were eluted from the ion exchange column.The possible reaction mechanism of the CL system was suggested and discussed.The response of the sensor to gallic acid concentration was linear over the range of 8.0×10-—1.0×10-6mol/L with a detection limit of 6.5×10-9 mol/L(3σ).The relative standard deviation (RSD) for 7 repetitive determinations of gallic acid(1.0×10-7mol/L) was 1.8?The sensor could be used for over 400 times determination with a good reproducibility.

  14. Clinical significance of detection of insulin and C peptide in type 2 diabetic patients by chemiluminescence immunoassay

    International Nuclear Information System (INIS)

    To explore the clinical significance of insulin and C peptide in type 2 diabetic patients, the serum insulin and C peptide levels in 50 type 2 diabetics patients and 30 health controls were determined by chemiluminescence immunoassay. The results showed that the serum levels of insulin and C peptide in patients were significantly higher than that of healthy controls (P<0.05). The peak level of blood sugar appeared at 1 hour after meals in healthy controls, while in patients appeared at 2 hours and didn't return to normal at 3 hours. The serum insulin and C peptide levels could be used to determine the function of islet β cells and to guide clinical treatment. (authors)

  15. Control of excitation and quenching in multi-colour electrogenerated chemiluminescence systems through choice of co-reactant.

    Science.gov (United States)

    Barbante, Gregory J; Kebede, Noah; Hindson, Christopher M; Doeven, Egan H; Zammit, Elizabeth M; Hanson, Graeme R; Hogan, Conor F; Francis, Paul S

    2014-10-20

    We demonstrate a new approach to manipulate the selective emission in mixed electrogenerated chemiluminescence (ECL) systems, where subtle changes in co-reactant properties are exploited to control the relative electron-transfer processes of excitation and quenching. Two closely related tertiary-amine co-reactants, tri-n-propylamine and N,N-diisopropylethylamine, generate remarkably different emission profiles: one provides distinct green and red ECL from [Ir(ppy)3] (ppy=2-phenylpyridinato-C2,N) and a [Ru(bpy)3](2+) (bpy=2,2'-bipyridine) derivative at different applied potentials, whereas the other generates both emissions simultaneously across a wide potential range. These phenomena can be rationalized through the relative exergonicities of electron-transfer quenching of the excited states, in conjunction with the change in concentration of the quenchers over the applied potential range.

  16. A biostatistical study into the efficiency of individualism using nonisotopic chemiluminescent-enhanced NICE multilocus DNA probes.

    Science.gov (United States)

    Hau, P P; Watt, E H; Hau, C M

    1997-10-01

    The efficiency of individualisation using nonisotopic chemiluminescent- enhanced probes (NICE) was investigated by analysing DNA fingerprints obtained from 190 unrelated Caucasians. Novel analysis of the scoring procedure enabled us to include the comparison of 585 pairs of samples for each of two probes. When the results of NICE probes 33.6 and 33.15 were combined, the mean percentage band share between two unrelated individuals was 16.8% and the mean number of bands identified in an individual DNA fingerprint was 54.8. Results were compared with those obtained using isotopically labelled probes and suggest that the two labelling systems gave similar efficiencies for differentiating between individuals. Analysis of DNA fingerprints from 37 family trios (mother, child and father groups) gave a mutation rate of 0.10% when using NICE probes. The two labelling systems compared were equally efficient in establishing family relationships.

  17. Improvement on Simultaneous Determination of Cr(III) and Cr(VI) by Capillary Electrophoresis and Chemiluminescence Detection

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    A sensitive method for the simultaneous determination of Cr(III) and Cr(VI) using in-capillary reaction capillary electrophoresis separation and chemiluminescence detection was developed. The procedures were designed as follows: The sample, hydrochloric acid and sodium hydrogen sulfite solution segments were injected sequentially into the capillary. The reaction of Cr(VI) reduced to Cr(III) by HSO3- occurred inside the capillary after applying the running voltage. According to the migration time difference of both Cr(III) ions moving towards to the cathode (detection end), they could be separated and determined. The limits of detection for chromium(III) and chromium(VI) (S/N = 3) were 6.0(10-13 mol/L (12 zmol) and 1.9(10-11 mol/L (380 zmol), respectively.

  18. Chemiluminescence Resonance Energy Transfer Competitive Immunoassay Employing Hapten-Functionalized Quantum Dots for the Detection of Sulfamethazine.

    Science.gov (United States)

    Ma, Mingfang; Wen, Kai; Beier, Ross C; Eremin, Sergei A; Li, Chenglong; Zhang, Suxia; Shen, Jianzhong; Wang, Zhanhui

    2016-07-20

    We describe a new strategy for using chemiluminescence resonance energy transfer (CRET) by employing hapten-functionalized quantum dots (QDs) in a competitive immunoassay for detection of sulfamethazine (SMZ). Core/multishell QDs were synthesized and modified with phospholipid-PEG. The modified QDs were functionalized with the hapten 4-(4-aminophenyl-sulfonamido)butanoic acid. The CRET-based immunoassay exhibited a limit of detection for SMZ of 9 pg mL(-1), which is >4 orders of magnitude better than a homogeneous fluorescence polarization immunoassay and is 2 orders of magnitude better than a heterogeneous enzyme-linked immunosorbent assay. This strategy represents a simple, reliable, and universal approach for detection of chemical contaminants.

  19. Chemiluminescence Resonance Energy Transfer Competitive Immunoassay Employing Hapten-Functionalized Quantum Dots for the Detection of Sulfamethazine.

    Science.gov (United States)

    Ma, Mingfang; Wen, Kai; Beier, Ross C; Eremin, Sergei A; Li, Chenglong; Zhang, Suxia; Shen, Jianzhong; Wang, Zhanhui

    2016-07-20

    We describe a new strategy for using chemiluminescence resonance energy transfer (CRET) by employing hapten-functionalized quantum dots (QDs) in a competitive immunoassay for detection of sulfamethazine (SMZ). Core/multishell QDs were synthesized and modified with phospholipid-PEG. The modified QDs were functionalized with the hapten 4-(4-aminophenyl-sulfonamido)butanoic acid. The CRET-based immunoassay exhibited a limit of detection for SMZ of 9 pg mL(-1), which is >4 orders of magnitude better than a homogeneous fluorescence polarization immunoassay and is 2 orders of magnitude better than a heterogeneous enzyme-linked immunosorbent assay. This strategy represents a simple, reliable, and universal approach for detection of chemical contaminants. PMID:27362827

  20. Liquid chromatography of antihistamines using post-column tris(2,2'-bipyridine) ruthenium(III) chemiluminescence detection.

    Science.gov (United States)

    Holeman, J A; Danielson, N D

    1994-09-23

    The separation and detection of five antihistamine drugs commonly found within over-the-counter allergy and cold pharmaceutical products was performed by HPLC with chemiluminescence (CL) detection. Comparable detection limits at 5-10 pmol were found for the antihistamines by both UV at 214 nm and tris(2,2'-bipyridine) ruthenium(III) CL. However, urine samples were found not to generate as large an unretained peak by CL detection as compared to those peaks by UV detection at 214 and 254 nm. For example, the pheniramine peak representing 0.15 microgram/ml was almost totally obscured at 214 nm. Quantitative results received for three antihistamine commercial samples ranged from 4 to 8% error in accuracy when an internal standard was used to compensate for short term detector drift.

  1. Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting

    Directory of Open Access Journals (Sweden)

    Luis Izquierdo

    2013-11-01

    Full Text Available The disappearance of lytic, protective antibodies (Abs from the serum of patients with Chagas disease is accepted as a reliable indicator of parasitological cure. The efficiency of a chemiluminescent enzyme-linked immunosorbent assay based on a purified, trypomastigote-derived glycosylphosphatidylinositol-anchored mucin antigen for the serologic detection of lytic Abs against Trypanosoma cruzi was evaluated in a nonendemic setting using a panel of 92 positive and 58 negative human sera. The technique proved to be highly sensitive {100%; 95% confidence interval (CI = 96-100} and specific (98.3%; 95% CI = 90.7-99.7, with a kappa score of 0.99. Therefore, this assay can be used to detect active T. cruzi infection and to monitor trypanosomicidal treatment.

  2. A portable chemiluminescence imaging immunoassay for simultaneous detection of different isoforms of prostate specific antigen in serum.

    Science.gov (United States)

    Liu, Anran; Zhao, Fang; Zhao, Yuewu; Shangguan, Li; Liu, Songqin

    2016-07-15

    A multianalyte chemiluminescence (CL) imaging immunoassay strategy for sensitive detection of different isoforms of prostate specific antigen (PSA) was developed. The microtiter plates were fabricated by simultaneously immobilizing of free-PSA (f-PSA) and total-PSA (t-PSA) capture antibody on nitrocellulose (NC) membrane. Each of the array were spotted in replicates of six spots within a spacing of 2mm. 16 or 48 detection wells were integrated on a single NC membrane and each well could be used as a microreactor and microanalysis chamber. Under a sandwiched immunoassay, the CL signals on each sensing site were collected by a charge-coupled device (CCD), presenting an array-based chemiluminescence imaging. Soybean peroxidase (SBP) was used to label f-PSA or t-PSA monoclonal antibody. With the amplification effects of two enhancers, 3-(10'-phenothiazinyl) propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORP), the CL intensity could significantly enhanced, which improved the sensing sensitivity and detection limit. Under the optimal conditions, the linear response to the analyte concentration ranged from 0.01-36.7ng/mL and 0.02-125ng/mL for f-PSA and t-PSA, respectively. The results for the detection of forty serum samples from prostate cancer patients and cancer-free patients showed good agreement with the clinical data, suggesting that the proposed assay had acceptable accuracy. The proposed CL imaging immunoassay possess high throughput and acceptable reproducibility, stability and accuracy, which made it great potential to available to distinguish different isoforms of PSA in serum samples. PMID:26922048

  3. A novel flow injection chemiluminescence determination of Cr(VI) with Dichlorotris(1,10-phenanthroline)ruthenium(II).

    Science.gov (United States)

    Luaces, M D; Martínez, N C; Granda, M; Valdés, A C; Pérez-Conde, C; Gutiérrez, A M

    2011-09-30

    A selective novel reverse flow injection system with chemiluminescence detection (rFI-CL) for the determination of Cr(VI) in presence of Cr(III) with Dichlorotris (1,10-phenanthroline)ruthenium(II), (Ru(phen)(3)Cl(2)), is described in this work. This new method is based on the oxidation capacity of Cr(VI) in H(2)SO(4) media. First, the Ruthenium(II) complex is oxidized to Ruthenium(III) complex by Cr(VI) and afterwards it is reduced to the excited state of the Ruthenium(II) complex by a sodium oxalate solution, emitting light inside the detector. The intensity of chemiluminescence (CL) is proportional to the concentration of Cr(VI) and, under optimum conditions, it can be determined over the range of 3-300 μg L(-1) with a detection limit of 0.9 μg L(-1). The RSD was 8.4% and 1.5% at 5 and 50 μg L(-1), respectively. For the rFI-CL method various analytical parameters were optimized: flow rate (1 mL min(-1)), H(2)SO(4) carrier concentration (20% w/V), Ru(phen)(3)Cl(2) concentration (5mM) and sodium oxalate concentration (0.1M). The effect of Cr(III), Fe(III), Al(III), Cd(II), Zn(II), Hg(II), Pb(II), Ca(II) and Mg(II), was studied. The method is highly sensitive and selective, allowing a fast, on-line determination of Cr(VI) in the presence of Cr(III). Finally, the method was tested in four different water samples (tap, reservoir, well and mineral), with good recovery percentage. PMID:21872036

  4. Simultaneous Determination of Size and Quantification of Gold Nanoparticles by Direct Coupling Thin layer Chromatography with Catalyzed Luminol Chemiluminescence

    Science.gov (United States)

    Yan, Neng; Zhu, Zhenli; He, Dong; Jin, Lanlan; Zheng, Hongtao; Hu, Shenghong

    2016-04-01

    The increasing use of metal-based nanoparticle products has raised concerns in particular for the aquatic environment and thus the quantification of such nanomaterials released from products should be determined to assess their environmental risks. In this study, a simple, rapid and sensitive method for the determination of size and mass concentration of gold nanoparticles (AuNPs) in aqueous suspension was established by direct coupling of thin layer chromatography (TLC) with catalyzed luminol-H2O2 chemiluminescence (CL) detection. For this purpose, a moving stage was constructed to scan the chemiluminescence signal from TLC separated AuNPs. The proposed TLC-CL method allows the quantification of differently sized AuNPs (13 nm, 41 nm and 100 nm) contained in a mixture. Various experimental parameters affecting the characterization of AuNPs, such as the concentration of H2O2, the concentration and pH of the luminol solution, and the size of the spectrometer aperture were investigated. Under optimal conditions, the detection limits for AuNP size fractions of 13 nm, 41 nm and 100 nm were 38.4 μg L-1, 35.9 μg L-1 and 39.6 μg L-1, with repeatabilities (RSD, n = 7) of 7.3%, 6.9% and 8.1% respectively for 10 mg L-1 samples. The proposed method was successfully applied to the characterization of AuNP size and concentration in aqueous test samples.

  5. Simultaneous Determination of Size and Quantification of Gold Nanoparticles by Direct Coupling Thin layer Chromatography with Catalyzed Luminol Chemiluminescence

    Science.gov (United States)

    Yan, Neng; Zhu, Zhenli; He, Dong; Jin, Lanlan; Zheng, Hongtao; Hu, Shenghong

    2016-01-01

    The increasing use of metal-based nanoparticle products has raised concerns in particular for the aquatic environment and thus the quantification of such nanomaterials released from products should be determined to assess their environmental risks. In this study, a simple, rapid and sensitive method for the determination of size and mass concentration of gold nanoparticles (AuNPs) in aqueous suspension was established by direct coupling of thin layer chromatography (TLC) with catalyzed luminol-H2O2 chemiluminescence (CL) detection. For this purpose, a moving stage was constructed to scan the chemiluminescence signal from TLC separated AuNPs. The proposed TLC-CL method allows the quantification of differently sized AuNPs (13 nm, 41 nm and 100 nm) contained in a mixture. Various experimental parameters affecting the characterization of AuNPs, such as the concentration of H2O2, the concentration and pH of the luminol solution, and the size of the spectrometer aperture were investigated. Under optimal conditions, the detection limits for AuNP size fractions of 13 nm, 41 nm and 100 nm were 38.4 μg L−1, 35.9 μg L−1 and 39.6 μg L−1, with repeatabilities (RSD, n = 7) of 7.3%, 6.9% and 8.1% respectively for 10 mg L−1 samples. The proposed method was successfully applied to the characterization of AuNP size and concentration in aqueous test samples. PMID:27080702

  6. The application of chemiluminescence in the detection of HCV/HIV%化学发光技术在HCV和HIV检测中的应用

    Institute of Scientific and Technical Information of China (English)

    李璐; 邢文革

    2011-01-01

    Various methods based on chemiluminescence for the detection of infections and course of diseases have been developed rapidly in the recent years, and are widely used in disease monitoring, screening and diagnosis. Technologies for the detection of HCV/HIV based on chemiluminescence have many advantages in comparison with other current technologies. Detection of HCV core antigen by chemiluminescence could result in not only a high sensitivity and specificity but also in a short window period; besides, by applying the linear relationship between the HCV RNA titers and the content of HCV core antigen in the samples, the HCV RNA titers are available. The sensitivity and specificity are both high and false positive rate is low when the chemiluminescence is used for detecting anti-HCV and anti-HIV. Chemiluminescence technology for testing the HCV/HIV nucleic can get a wide detection limit and linear range. In addition, the virus mutation and activity of reverse transcription can also be detected by the chemiluminescence technology. The chemiliminescence technology can be broadly used in detecting of HCV/HIV.%近年来利用化学发光技术检测疾病的感染状况及疾病进程的方法得到了迅速的发展,并在疾病的监测、筛查、诊断的方面得到了广泛应用.结合化学发光技术的丙型肝炎病毒(HCV)/艾滋病病毒(HIV)检测方法,具有很多优于现有检测手段的特点.化学发光技术用于检测HCV核心抗原不仅灵敏度、特异度高、窗口期短,而且可根据HCV核心抗原与RNA滴度间的线性关系,计算得出样本中的HCV RNA滴度;用于检测抗-HCV、抗-HIV检测时具有灵敏度高、特异度高、假阳性率低的优点.在HCV/HIV核酸检测方面,化学发光技术的检测限及线性范围更宽.此外,利用化学发光技术还可以对反转录酶活性及病毒的变异进行检测.化学发光技术在HCV/HIV的检测领域具有广泛的应用前景.

  7. Chemiluminescent methods and instruments for monitoring of the atmosphere and satellite validation on board of research aircrafts and unmanned aerial vehicles

    Science.gov (United States)

    Sitnikov, Nikolay; Borisov, Yuriy; Akmulin, Dimitry; Chekulaev, Igor; Sitnikova, Vera; Ulanovsky, Alexey; Sokolov, Alexey

    The results of development of instruments based on heterophase chemiluminescence for measurements of space distribution of ozone and nitrogen oxides concentrations on board of research aircrafts and unmanned aerial vehicles carried out in Central Aerological Observatory are presented. Some results of atmospheric investigations on board of research aircrafts M55 “Geophysica” (Russia) and “Falcon” (Germany) carried out using developed instruments in frame of international projects are demonstrated. Small and low power instruments based on chemiluminescent principle for UAV are developed. The results of measurements on board of UAV are shown. The development can be used for satellite data validation, as well as operative environmental monitoring of contaminated areas in particular, chemical plants, natural and industrial disasters territories, areas and facilities for space purposes etc.

  8. High performance liquid chromatography with immobilized Ru(bpy)32+-KMn04 chemiluminescence detection and its application in metabolism of repaglinide in pig liver microsomes

    Institute of Scientific and Technical Information of China (English)

    Ai Hua Fu; Zhu Jun Zhang; Li Li Chen; Xiao Ming Zhang; Pan Xue

    2011-01-01

    A novel high performance liquid chromatography-chemiluminescence (HPLC-CL) method for investigation of in vitro metabolism of repaglinide in pig liver microsomes with microdialysis sampling technique was developed. The chromatographic separation was performed on a Hypersil BDS-C18 column with an isocratic mobile phase consisting of methanol and 0.01 mol/L KH2PO4 (pH 3.0) (volume ratio 75:25) at a flow rate of 1.0 mL/min. The detection was based on the chemiluminescence reaction of repaglinide with acidic potassium permanganate (KMnO4) and tris (2,2'-bipyridyl)ruthenium(III) (Ru(bpy)33+), which was immobilized on the cationic ion-exchange resin for obtaining high sensitivity and reducing consumption of expensive reagent.

  9. Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III-Tetrakis (4-Sulfonatophenyl Porphyrin-Luminol-Hydrogen Peroxide System

    Directory of Open Access Journals (Sweden)

    Sayed Yahya Kazemi

    2012-01-01

    Full Text Available The present work deals with an attempt to study the effect of human and bovine serum albumin on kinetic parameters of chemiluminescence of luminol-hydrogen peroxide system catalyzed by manganese tetrasulfonatophenyl porphyrin (MnTSPP. The investigated parameters involved pseudo-first-order rise and fall rate constant for the chemiluminescence burst, maximum level intensity, time to reach maximum intensity, total light yield, and values of the intensity at maximum CL which were evaluated by nonlinear least square program KINFIT. Because of interaction of metalloporphyrin with proteins, the CL parameters are drastically affected. The systems resulted in Stern-Volmer plots with values of 3.17×105 and 3.7×105M−1 in the quencher concentration range of 1.5×10−6 to 1.5×10−5 M for human serum albumin (HSA and bovine serum albumin (BSA, respectively.

  10. A new chemiluminescence method for determination of clonazepam and diazepam based on 1-Ethyl-3-Methylimidazolium Ethylsulfate/copper as catalyst

    Science.gov (United States)

    Chaichi, M. J.; Alijanpour, S. O.

    2014-01-01

    A novel chemiluminescence (CL) reaction, Benzodiazepines-H2O2-1-Ethyl-3-Methylimidazolium Ethylsulfate/copper, for determination of clonazepam and diazepam at nanogram per milliliter level in batch-type system have been described. The method relies on the catalytic effect of 1-Ethyl-3-Methylimidazolium Ethylsulfate/copper on the chemiluminescence reaction of Benzodiazepines, the oxidation of Benzodiazepines with hydrogen peroxide in natural medium. The influences of various experimental parameters such as solution pH, the ratio of 1-Ethyl-3 Methylimidazolium ethylsulfate concentration to copper ion, the type of buffer and the concentration of CL reagents were investigated. Under the optimum condition, the proposed method was satisfactorily applied for the determination of these drugs in tablets and urine without the interference of their potential impurities.

  11. Electrogenerated chemiluminescence quenching of Ru(bpy)32+ (bpy=2,2′-bipyridine) in the presence of acetaminophen, salicylic acid and their metabolites

    International Nuclear Information System (INIS)

    Quenching of Ru(bpy) 32+ (bpy=2,2′-bipyridine) coreactant electrogenerated chemiluminescence (ECL) has been observed in the presence of acetaminophen, salicylic acid and related complexes. However, no quenching is observed with the acetylsalicylic acid. In most instances, quenching is observed with 100-fold excess of quencher (compared to ECL luminophore) with complete quenching observed between 10,000 and 100,000 fold excess. Fluorescence and UV–vis experiments coupled with bulk electrolysis support the formation of benzoquinone products upon electrochemical oxidation. The mechanism of quenching may involve the interaction of the electrochemically generated benzoquinone species with (i) the ⁎Ru(bpy)32+ excited state or (ii) highly energetic coreactant radicals. - Highlights: ▶ Efficient quenching of the electrogenerated chemiluminescence is observed. ▶ Acetaminophen, salicylic acid and related compounds can be detected. ▶ The mechanism of quenching involves benzoquinones formed upon electrolysis.

  12. Radiation-Induced Graft Polymerization of Vinyl Monomers with Anion Groups onto MWNT Supports and Their Application as Electrogenerated Chemiluminescence (ECL Biosensors

    Directory of Open Access Journals (Sweden)

    Ji-Hye Park

    2014-01-01

    Full Text Available Vinyl polymer-grafted multiwalled carbon nanotube (MWNT supports with anion groups were prepared for use as biosensor supports by radiation-induced graft polymerization (RIGP of the vinyl monomers acryloyl diphosphoric acid (ADPA, acrylic acid (AA, sodium styrenesulfonate (NaSS, and methacrylic acid (MA onto the surface of MWNTs. The electrogenerated chemiluminescence sensors based on a glass carbon electrode (ECL-GCE and a screen printed electrode (ECL-SPE were fabricated by immobilization of Ru(bpy3 2+ complex after coating of vinyl polymer-grafted MWNT inks on the surface of the GCE and SPE without any polymer binders in order to obtain high electrogenerated chemiluminescence intensity. For detection of alcohol concentration, alcohol dehydrogenase (ADH was immobilized onto an ECL-GCE sensor prepared by poly(NaSS-g-MWNT supports. The prepared biosensor based on ADH is suitable for the detection of ethanol concentration in commercial drinks.

  13. Evaluation of a von Willebrand factor three test panel and chemiluminescent-based assay system for identification of, and therapy monitoring in, von Willebrand disease.

    Science.gov (United States)

    Favaloro, Emmanuel J; Mohammed, Soma

    2016-05-01

    von Willebrand disease (VWD) is reportedly the most common bleeding disorder and arises from deficiency and/or defects of von Willebrand factor (VWF). Laboratory diagnosis and typing of VWD has important management implications and requires a wide range of tests, including VWF antigen (VWF:Ag) and various activities, involving differential identification of qualitative vs quantitative VWF defects. We have assessed a new hemostasis instrument, the chemiluminescent assay based ACL AcuStar™, and an associated HemosIL AcuStar three test panel comprising VWF:Ag, VWF ristocetin cofactor (VWF:RCo) and VWF collagen binding (VWF:CB) (Instrumentation Laboratory, Bedford, Ma. USA) for ability to identify VWD, to help provisionally type VWD, and for potential use in therapy monitoring. This test system was compared to previously evaluated and validated test systems including VWF:RCo on CS-5100 and BCS analyzers, the new Siemens INNOVANCE assay (VWF Ac) on CS-5100, and VWF:Ag and VWF:CB assays performed by automated ELISA. We employed a large total sample test set (n=535) comprising plasma and platelet-lysate samples from individuals with and without VWD, some on treatment, normal plasmas, and normal and pathological controls. We also evaluated desmopressin (DDAVP) responsiveness, plus differential sensitivity to reduction in high molecular weight (HMW) VWF. The chemiluminescent test panel (VWF:Ag, VWF:RCo, VWF:CB) showed good comparability to similar assays performed by alternate methods, and broadly similar data for identification of VWD, provisional VWD type identification, DDAVP and VWD therapy, and HMW VWF sensitivity, although some notable differences were evident. The chemiluminescent system showed best low level VWF sensitivity, and lowest inter-assay variability, compared to all other systems. In conclusion, we have validated theACL AcuStar and the chemiluminescent HemosIL AcuStar VWF test panel for use in VWD diagnostics, and have identified some favorable

  14. On-Chip Isothermal Nucleic Acid Amplification on Flow-Based Chemiluminescence Microarray Analysis Platform for the Detection of Viruses and Bacteria.

    Science.gov (United States)

    Kunze, A; Dilcher, M; Abd El Wahed, A; Hufert, F; Niessner, R; Seidel, M

    2016-01-01

    This work presents an on-chip isothermal nucleic acid amplification test (iNAAT) for the multiplex amplification and detection of viral and bacterial DNA by a flow-based chemiluminescence microarray. In a principle study, on-chip recombinase polymerase amplification (RPA) on defined spots of a DNA microarray was used to spatially separate the amplification reaction of DNA from two viruses (Human adenovirus 41, Phi X 174) and the bacterium Enterococcus faecalis, which are relevant for water hygiene. By establishing the developed assay on the microarray analysis platform MCR 3, the automation of isothermal multiplex-amplification (39 °C, 40 min) and subsequent detection by chemiluminescence imaging was realized. Within 48 min, the microbes could be identified by the spot position on the microarray while the generated chemiluminescence signal correlated with the amount of applied microbe DNA. The limit of detection (LOD) determined for HAdV 41, Phi X 174, and E. faecalis was 35 GU/μL, 1 GU/μL, and 5 × 10(3) GU/μL (genomic units), which is comparable to the sensitivity reported for qPCR analysis, respectively. Moreover the simultaneous amplification and detection of DNA from all three microbes was possible. The presented assay shows that complex enzymatic reactions like an isothermal amplification can be performed in an easy-to-use experimental setup. Furthermore, iNAATs can be potent candidates for multipathogen detection in clinical, food, or environmental samples in routine or field monitoring approaches.

  15. A dual-readout chemiluminescent-gold lateral flow test for multiplex and ultrasensitive detection of disease biomarkers in real samples.

    Science.gov (United States)

    Chen, Yiping; Sun, Jiashu; Xianyu, Yunlei; Yin, Binfeng; Niu, Yajing; Wang, Songbai; Cao, Fengjing; Zhang, Xiaoqing; Wang, Yu; Jiang, Xingyu

    2016-08-18

    Even though the gold lateral flow test (GLFT) is low-cost and allows for point-of-care testing (POCT), its intrinsic limitations including low sensitivity and incapability of quantification significantly hinder the clinical application of GLFT for assaying disease biomarkers. To improve the performance of the GLFT without sacrificing its simplicity, we develop a chemiluminescent-gold lateral flow test (C-mode GLFT) for quantitative and multiplex detection of disease biomarkers with an ultrahigh sensitivity at a picomolar level. Horseradish peroxidase (HRP) and antibody (Ab) are simultaneously labeled onto the surface of gold nanoparticles (AuNPs) to achieve a dual-readout (chemiluminescent and visual, C&V-mode GLFT). A red color appears at the test line caused by the accumulation of captured AuNPs in the presence of targets, while HRP on the surface of AuNPs catalyzes the chemiluminescence reaction of luminol to amplify the signal. C-mode GLFT is successfully used for detecting tumor biomarkers (alpha fetoprotein, AFP, and carcino embryonic antigen, CEA) and bacterial infection biomarkers (procalcitonin, PCT) in serum samples as well as whole blood. The excellent features of C-mode GLFT such as straightforward operation, ultrahigh sensitivity and quantitative detection, make it a promising platform for POCT of a variety of disease biomarkers in real samples. PMID:27375054

  16. Determination of the pseudoephedrine content in pharmaceutical formulations and in biological fluids using a microbore HPLC system interfaced to a microfluidic chemiluminescence detector.

    Science.gov (United States)

    Kadavilpparampu, Afsal Mohammed; Al-Lawati, Haider A J; Suliman, FakhrEldin O; Al Kindy, Salma M Z

    2015-12-01

    A novel automated precolumn derivatization followed by separation using liquid chromatography for the determination of pseudoephedrine (PSE) by a microfluidic chemiluminescence detector has been developed. An on-line derivatization procedure was utilized by converting PSE into a highly light emitting species in a Ru(bipy)3(2+)-peroxydisulphate chemiluminescence (CL) system by derivatizing it with a 1.0 M formaldehyde solution. The derivatized analyte was directly injected into a microbore high-performance liquid chromatography (HPLC) system coupled to an on-chip chemiluminescence detector. The newly developed highly selective, sensitive and fast HPLC-CL method was validated and successfully applied for the analysis of PSE in pharmaceutical formulations and a human urine sample. The selectivity of the method is not only due to the HPLC separation but is also due to the highly selective detection principle of the Ru(bipy)3(2+)-peroxydisulphate CL system used. There was no interference observed from the common preservatives and excipients used in pharmaceutical preparations, which did not show any significant CL signal. The retention time of PSE was less than 3 min, and the detection limits and quantification limits were found to be 5.7 and 26.0 µg L(-1), respectively.

  17. Chemiluminescence ELISA for the detection of oxidative DNA base damage using anti-8-hydroxy-2'-deoxyguanosine antibody. Application to the detection of irradiated foods

    International Nuclear Information System (INIS)

    Since ionizing radiation is used for sterilizing or lowering the microbial content of foods as a means of reducing food losses and securing food safety, the development of versatile detection methods of irradiated foods is necessary for appropriate management. In an effort to distinguish between irradiated and non-irradiated food, a method based on the detection of oxidative DNA base damage using the chemiluminescence enzyme-linked immunosorbent assay (ELISA) with anti-8-hydroxy-2'-deoxyguanosine antibody was developed. In the course of optimizing the reaction conditions for the ELISA, a 30-mer synthetic oligonucleotide containing 8-hydroxyguanine (8-oxoG) was used. Under the optimized conditions, the correlation between chemiluminescence intensity and 8-oxoG content in oligonucleotides was obtained. It was shown that this chemiluminescence ELISA method could be applied to chicken, beef and pork that were irradiated with over 3 kGy. Twenty milligrams of a loaf of meat was sufficient to distinguish between irradiated and non-irradiated meat by this method. (author)

  18. A novel polydopamine-based chemiluminescence resonance energy transfer method for microRNA detection coupling duplex-specific nuclease-aided target recycling strategy.

    Science.gov (United States)

    Wang, Qian; Yin, Bin-Cheng; Ye, Bang-Ce

    2016-06-15

    MicroRNAs (miRNAs), functioning as oncogenes or tumor suppressors, play significant regulatory roles in regulating gene expression and become as biomarkers for disease diagnostics and therapeutics. In this work, we have coupled a polydopamine (PDA) nanosphere-assisted chemiluminescence resonance energy transfer (CRET) platform and a duplex-specific nuclease (DSN)-assisted signal amplification strategy to develop a novel method for specific miRNA detection. With the assistance of hemin, luminol, and H2O2, the horseradish peroxidase (HRP)-mimicking G-rich sequence in the sensing probe produces chemiluminescence, which is quickly quenched by the CRET effect between PDA as energy acceptor and excited luminol as energy donor. The target miRNA triggers DSN to partially degrade the sensing probe in the DNA-miRNA heteroduplex to repeatedly release G-quadruplex formed by G-rich sequence from PDA for the production of chemiluminescence. The method allows quantitative detection of target miRNA in the range of 80 pM-50 nM with a detection limit of 49.6 pM. The method also shows excellent specificity to discriminate single-base differences, and can accurately quantify miRNA in biological samples, with good agreement with the result from a commercial miRNA detection kit. The procedure requires no organic dyes or labels, and is a simple and cost-effective method for miRNA detection for early clinical diagnosis. PMID:26866561

  19. СHANGES IN PARAMETERS OF LUMINOL-DEPENDENT AND LUCIGENIN-DEPENDENT CHEMILUMINESCENCE OF PERIPHERAL BLOOD NEUTROPHILS IN PATIENTS WITH BLADDER CANCER IN THE DISEASE DYNAMICS

    Directory of Open Access Journals (Sweden)

    L. M. Kurtasova

    2015-01-01

    Full Text Available The study deals with parameters of luminol-dependent and lucigenin-dependent chemiluminescence (CL of peripheral blood neutrophils from patients with bladder cancer (BC prior to surgical treatment. We examined sixty patients (45 to 55 years old with advanced bladder cancer (TNM prior to the operation, and forty-six patients at 10 days after surgical treatment. A control group consisted of 56 healthy donors. Luminol-dependent and lucigenin-dependent chemiluminescence of blood neutrophils was assessed according to De Sole et al. (1983. Chemiluminescence assays of peripheral blood neutrophils from the patients with bladder cancer revealed changes in production of reactive oxygen species (ROS, both for initial stage of oxidation reaction, and total level of active oxygen radicals. We have found disturbed values of primary-to-secondary ROS ratio in the cells. In the patients with bladder cancer, some changes in oxidative metabolism of the blood neutrophils have been registered. These alterations may play an important role in promotion of potential effector cell functions, thus, probably, affecting the whole-scale development of a cytopathic effect exerted by neutrophilic granulocytes. 

  20. Synthesis and physicochemical properties of novel lophine derivatives as chemiluminescent in vitro activators for detection of free radicals.

    Science.gov (United States)

    Pavlova, Elitsa; Kaloyanova, Stefka; Deligeorgiev, Todor; Lesev, Nedyalko

    2015-12-01

    The overproduction of free radicals and reactive oxygen species (ROS) has been proved as a basic damage mechanism and cause for oxidative stress. Their measurement is often hindered by the low signal. This could be resolved with the application of luminescent probes (lophines, luminol, lucigenin, etc.). The focus of this study is to synthesize and describe the spectral properties and physicochemical characteristics of lophine and its derivatives as new chemiluminescent in vitro activators. The prepared luminophores are analogues of lophine. Their absorption maxima are in the range 329-340 nm, with good-to-high extinction coefficients. Their spectral properties are measured in methanol and buffer solutions with pH 3.5, 7.4 and 8.5. Same conditions were applied in the systems for chemiluminescent assay in vitro: (1) Fenton's (Fe(2+)+H2O2) for the generation of ·OH and -OH species, (2) Hydrogen peroxide (H2O2), (3) Iron (II) sulfate (FeSO4), (4) Glutathione-peroxidase, monitoring the deactivation of H2O2, (5) Ascorbic acid-Fenton's reagent: Vit.C appears a strong oxidant, generating free-radical products when applied in higher than physiological concentrations, (6) Reduced α-nicotinamide adenine dinucleotide (NADH)-phenazine methosulfate-for the generation of superoxide radicals (O2 (·-)). Lophine and all novel compounds do not alter the kinetics, except of the dimethyl amino substituted derivative (4-(3a,11b-dihydro-1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)-N,N-dimethylaniline) in the glutathione-peroxidase system, at pH 8.5. Same derivative showed a comparable or higher activity than Lucigenin and Rhodamine 6G. In neutral and acidic medium, in the Fenton's system, Rhodamine 6G was the most appropriate probe. In alkaline pH and oxidant H2O2, Lucigenin induced a signal twice as strong as the signal compared to all other activators. PMID:26224302

  1. Chemiluminescence flow biosensor for glucose using Mg-Al carbonate layered double hydroxides as catalysts and buffer solutions.

    Science.gov (United States)

    Wang, Zhihua; Liu, Fang; Lu, Chao

    2012-01-01

    In this work, serving as supports in immobilizing luminol reagent, catalysts of luminol chemiluminescence (CL), and buffer solutions for the CL reaction, Mg-Al-CO(3) layered double hydroxides (LDHs) were found to trigger luminol CL in weak acid solutions (pH 5.8). The silica sol-gel with glucose oxidase and horseradish peroxidase was immobilized in the first half of the inside surface of a clear quartz tube, and luminol-hybrid Mg-Al-CO(3) LDHs were packed in the second half. Therefore, a novel CL flow-through biosensor for glucose was constructed in weak acid solutions. The CL intensity was linear with glucose concentration in the range of 0.005-1.0mM, and the detection limit for glucose (S/N=3) was 0.1 μM. The proposed biosensor exhibited excellent stability, high reproducibility and high selectivity for the determination of glucose and has been successfully applied to determine glucose in human plasma samples with satisfactory results. The success of this work has broken the bottleneck of the pH incompatibility between luminol CL and enzyme activity. PMID:22770831

  2. A novel strategy for synthesis of hollow gold nanosphere and its application in electrogenerated chemiluminescence glucose biosensor.

    Science.gov (United States)

    Zhong, Xia; Chai, Ya-Qin; Yuan, Ruo

    2014-10-01

    Well-distributed hollow gold nanospheres (Aushell@GOD) (20 ± 5 nm) were synthesized using the glucose oxidase (GOD) cross-linked with glutaraldehyde as a template. A glucose biosensor was prepared based on Aushell@GOD nanospheres for catalyzing luminol electrogenerated chemiluminescence (ECL). Firstly, chitosan was modified in a glassy carbon electrode which offered an interface of abundant amino-groups to assemble Aushell@GOD nanospheres. Then, glucose oxidase was adsorbed on the surface of Aushell@GOD nanospheres via binding interactions between Aushell and amino groups of GOD to construct a glucose biosensor. The Aushell@GOD nanospheres were investigated with TEM and UV-vis. The ECL behaviors of the biosensor were also investigated. Results showed that, the obtained Aushell@GOD nanospheres exhibited excellent catalytic effect towards the ECL of luminol-H2O2 system. The response of the prepared biosensor to glucose was linear with the glucose concentration in the range of 1.0 μM to 4.3mM (R=0.9923) with a detection limit of 0.3 μM (signal to noise=3). This ECL biosensor exhibited short response time and excellent stability for glucose. At the same time the prepared ECL biosensor showed good reproducibility, sensitivity and selectivity. PMID:25059123

  3. Comprehensive two-dimensional gas chromatography coupled with fast sulphur-chemiluminescence detection: implications of detector electronics.

    Science.gov (United States)

    Blomberg, Jan; Riemersma, Toby; van Zuijlen, Manfred; Chaabani, Hassan

    2004-09-24

    Within the petrochemical industry, there has been a growing interest in methods capable of providing detailed information on the distribution of sulphur-containing compounds in various product streams, going down to the level of separating and quantifying individual sulphur species. Since no single capillary gas chromatographic column is able to perform this separation, a refuge to multi-dimensional separation techniques has to be taken. In this respect, comprehensive two-dimensional gas chromatography (GC x GC) coupled with sulphur chemiluminescence detection (SCD) has shown to be highly promising. It has been suggested, however, that the detector volume of an SCD restricts its potential to keep up with the fast second-dimension separations of contemporary GC x GC. In this paper, we will demonstrate that the lack of speed of the SCD does not originate from its physical dimensions, but is largely determined by the speed of the electronics used. Additionally, some typical examples will be presented to illustrate the potential of GC x GC coupled with fast SCD.

  4. Determination of organothiophosphorus pesticides in water by liquid chromatography and post-column chemiluminescence with cerium(IV).

    Science.gov (United States)

    Catalá-Icardo, Mónica; Lahuerta-Zamora, Luis; Torres-Cartas, Sagrario; Meseguer-Lloret, Susana

    2014-05-01

    A new, fast, selective and sensitive method has been developed for the simultaneous determination of nine organothiophosphorus (OTP) pesticides, namely omethoate, dimethoate, disulfoton-sulfoxide, methidathion, phosmet, malathion, diazinon, pirimiphos-methyl and chlorpyrifos. The pesticides were separated on a Kinetex C18 column by gradient elution with acetonitrile:water. A post-column basic hydrolysis of the pesticides and later a chemiluminescence (CL) reaction with cerium (IV) in acid medium was carried out. Hexadecylpyridinium chloride highly enhanced the CL emission. Under optimized conditions, linearity, precision, limits of detection and quantification, and accuracy were determined. Both selectivity and sensitivity were compared with those obtained with UV detection. In combination with SPE, limits of detection in the range 15-80ng/L and 5-30ng/L were obtained when 250mL and 1000mL of solution were treated, respectively. When applied to 250mL of sample the inter-day precision of the method was between 3.5% and 7.3% and the intra-day precision between 2.9% and 6.0%. The method was applied to determine OTP pesticides in spiked water samples from different origins: irrigation, river, sea, ground, spring, mineral and tap waters, being the percentage of recovery of added amounts near 100% form most of the pesticides.

  5. Silver nanoparticles enhanced a novel TCPO-H2O2-safranin O chemiluminescence system for determination of 6-mercaptopurine

    Science.gov (United States)

    Biparva, Pourya; Abedirad, Seyed Mohammad; Kazemi, Sayed Yahya

    2015-06-01

    The present study deals with first attempt to introduce safranin O as the fluorophore for peroxyoxalate chemiluminescence system. The reaction of bis-(2,4,6-trichlorophenyl) oxalate (TCPO) with H2O2 catalyzed by silver nanoparticles can transfer energy to safranin O via the formation of dioxetanedione intermediate and emits orange-red light. The relationship between CL intensity and the concentration of TCPO, fluorophore, hydrogen peroxide and nanocatalyst was investigated. The Ag nanoparticles were synthesized by chemical reduction method and characterized using scanning electron microscopy, particle size analyzer and UV-spectroscopy. Moreover, the system was applied successfully to detect a drug, 6-mercaptopurine (6-MP) in pharmaceuticals. Under optimum conditions, a linear working range for 6-MP concentrations from 5.5 × 10-7 to 5.5 × 10-5 mol L-1 (r > 0.9831, n = 6) was obtained with a detection limit of 1.6 × 10-7 mol L-1. The relative standard deviation for 6 repetitive determinations was less than 3.8% and recoveries of 98% and 103% were obtained.

  6. Clinical Evaluation of the ZstatFlu-II Test: a Chemiluminescent Rapid Diagnostic Test for Influenza Virus

    Science.gov (United States)

    Hamilton, Marilyn S.; Abel, David M.; Ballam, Yolanda J.; Otto, Mary K.; Nickell, Angela F.; Pence, Lisa M.; Appleman, James R.; Shimasaki, Craig D.; Achyuthan, Komandoor E.

    2002-01-01

    Exploiting the high sensitivity of the chemiluminescence phenomenon, an accurate and sensitive point-of-care test, called the ZstatFlu-II test (ZymeTx, Inc., Oklahoma City, Okla.), was developed to detect influenza virus infections. The ZstatFlu-II test takes 20 min and requires approximately 2 min of “hands-on” time for operational steps. The ZstatFlu-II test does not distinguish between infections with influenza virus types A and B. ZstatFlu-II test results are printed on Polaroid High-Speed Detector Film, allowing test results to be archived. A prototype version of the ZstatFlu-II test was evaluated during the 2000-to-2001 flu season with 300 nasal aspirate specimens from children at a pediatric hospital. Compared to culture, the ZstatFlu-II test had 88% sensitivity and 92% specificity. The Directigen test had a sensitivity of 75% and a specificity of 93%. The sensitivity of the ZstatFlu-II test was significantly higher than that of the Directigen test (P < 0.0574). PMID:12089243

  7. Chemiluminescence Determination of Tetracycline and Oxytetracycline in Pharmaceutical Preparations Using Ru(bipy)32+-Cerium(Ⅳ) System

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    A new chemiluminescence (CL) method for the determination of tetracycline and oxytetracycline is developed, based on the CL reaction of tetracycline and oxytetracycline with Ru(bipy)32+ and Ce (Ⅳ). In sulfuric acid medium, the CL emission is generated upon continuous oxidation of Ru(bipy)32+ by cerium (Ⅳ).The emission intensity is greatly enhanced when tetracycline and oxytetracycline are introduced into the reaction system after acid degradation. Under the optimum conditions, the calibration curves are linear over therange of 8. 0× 10-8~4. 0× 10-6 mol/L for tetracycline and of 2. 0× 10-7~4. 0× 10-5 mol/L for oxytetracycline, with the detection limits are 4. 2 × 10-8 mol/L for tetracycline and 1.5 × 10-7 mol/L for oxytetracydine, respectively. The proposed method was used for the determination of tetracycline and oxytetracycline in pharmaceutical formulations with good results.

  8. Electrogenerated chemiluminescence: An oxidative-reductive mechanism between quinolone antibiotics and tris(2,2'-bipyridyl)ruthenium(II)

    Energy Technology Data Exchange (ETDEWEB)

    Burkhead, Matthew S.; Wang, Heeyoung; Fallet, Marcel [Department of Chemistry, Creighton University, Omaha, NE 68178 (United States); Gross, Erin M. [Department of Chemistry, Creighton University, Omaha, NE 68178 (United States)], E-mail: eringross@creighton.edu

    2008-04-21

    The cyclic voltammetry and electrogenerated chemiluminescent (ECL) reactions of a series of quinolone and fluoroquinolone antibiotics were investigated in a flow injection analysis (FIA) system. 7-Piperazinyl fluoroquinolone antibiotics were found to participate as a coreactant in an oxidative-reductive ECL mechanism with tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy){sub 3}{sup 2+}) as the luminescent reagent. The reaction mechanism was investigated in order to understand and optimize the processes leading to light emission. The optimal conditions included a solution pH {approx}7 at a flow rate of 3.0 mL min{sup -1} with no added organic modifier and application of 1.2 V vs. a Pt quasi-reference electrode (QRE). Fluoroquinolones containing a tertiary distal nitrogen on the piperazine ring, such as enrofloxacin and ofloxacin, reacted to produce more intense ECL than those with a secondary nitrogen, such as ciprofloxacin and norfloxacin. The method linear range, precision, detection limits, and sensitivity for the detection of enrofloxacin and ciprofloxacin were compared to that of tripropylamine. The method was applied to the determination of the ciprofloxacin content in a pharmaceutical preparation. The assay is discussed in terms of its analytical figures of merit, ease of use, speed, accuracy and application to pharmaceutical samples.

  9. Determination of vitamin K homologues by high-performance liquid chromatography with on-line photoreactor and peroxyoxalate chemiluminescence detection

    Energy Technology Data Exchange (ETDEWEB)

    Ahmed, Sameh [Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521 (Japan); Kishikawa, Naoya [Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521 (Japan); Nakashima, Kenichiro [Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521 (Japan); Kuroda, Naotaka [Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521 (Japan)]. E-mail: n-kuro@nagasaki-u.ac.jp

    2007-05-22

    A sensitive and highly selective high-performance liquid chromatography (HPLC) method was developed for the determination of vitamin K homologues including phylloquinone (PK), menaquinone-4 (MK-4) and menaquinone-7 (MK-7) in human plasma using post-column peroxyoxalate chemiluminescence (PO-CL) detection following on-line ultraviolet (UV) irradiation. The method was based on ultraviolet irradiation (254 nm, 15 W) of vitamin K to produce hydrogen peroxide and a fluorescent product at the same time, which can be determined with PO-CL detection. The separation of vitamin K by HPLC was accomplished isocratically on an ODS column within 35 min. The method involves the use of 2-methyl-3-pentadecyl-1,4-naphthoquinone as an internal standard. The detection limits (signal-to-noise ratio = 3) were 32, 38 and 85 fmol for PK, MK-4 and MK-7, respectively. The recoveries of PK, MK-4 and MK-7 were greater than 82% and the inter- and intra-assay R.S.D. values were 1.9-5.4%. The sensitivity and selectivity of this method were sufficient for clinical and nutritional applications.

  10. Simultaneous Determination of Epinephrine, Noradrenaline and Dopamine in Human Serum Samples by High Performance Liquid Chromatography with Chemiluminescence Detection

    Institute of Scientific and Technical Information of China (English)

    CHEN, Fu-Nan; ZHANG, Ying-Xue; ZHANG, Zhu-Jun

    2007-01-01

    A simple, rapid and accurate high performance liquid chromatographic (HPLC) technique coupled with chemiluminescence (CL) detection was developed for the simultaneous determination of epinephrine (E),noradrenaline (NA) and dopamine (DA). It was based on the analyte enhancement effect on the CL reaction between luminol and potassium ferricyanide. The effects of various parameters, such as potassium ferricyanide concentration, luminol concentration, pH value and component of the mobile phase on chromatographic behaviors of the analytes (E, NA and DA) were investigated. The separation was carried out on C18 column using the mobile tions, E, NA and DA showed good linear relationships in the range of 1×10-8-5×10-6, 5.0×10-9-1.0× 10-6 and 5.0×10-9-1.0×10-6 g/mL respectively. The detection limits for E, NA and DA were 4.0×10-9, 1.0×10-9 and 8.0×10-10 g/mL. The proposed method has been applied successfully to the analysis of E, NA and DA in human serum samples.

  11. Lucigenin chemiluminescence assay as an adjunctive tool for assessment of various stages of inflammation: A study of quiescent inflammatory cells

    Indian Academy of Sciences (India)

    Knox Van Dyke; Shaily Patel; Val Vallyathan

    2003-02-01

    A simple, fast, precise and biologically relevant toxicity assay for screening cytotoxicity of minerals would have distinct advantages due to its cost benefits and relative savings in time. Furthermore, a bioassay to differentiate acute and chronic in vivo pulmonary reactions could have potential value as predictors of fibrogenicity and pathogenicity. In this study we examined the potential use of lucigenin as a probe to evaluate the correlation between chemiluminescence (CL) generated by alveolar macrophages with the known cytotoxicity and pathogenicity by conventional bioassays. In this study, we used small doses of dust (20 g) to minimize cellular overload and to maintain homeostasis. Crystalline silica a highly fibrogenic dust was used as positive control and results are compared with those for bentonite, kaolin and talc. Among the three minerals compared with silica, bentonite was more reactive (27%) in CL assay and declined sharply compared to other minerals. This sudden decline in bentonite CL is caused by cytotoxicity leading to cell death. CL-induced by talc was comparable to silica and declines slowly. Kaolin on the other hand produced relatively a weaker (25%) CL compared to silica. Our data using relatively low doses of dust suggest that the CL assay may have a better predictive value in cytotoxicity evaluations compared to conventional toxicity assays.

  12. A comprehensive evaluation of three microfluidic chemiluminescence methods for the determination of the total phenolic contents in fruit juices.

    Science.gov (United States)

    Al Haddabi, Buthaina; Al Lawati, Haider A J; Suliman, FakhrEldin O

    2017-01-01

    Three recently reported microfluidic chemiluminescence (MF-CL) methods (based on reactions with acidic permanganate enhanced by formaldehyde (KMnO4-COH), acidic cerium (IV) and rhodamine B (Ce-RB), and acidic cerium (IV) and rhodamine 6G (Ce-R6G) enhanced by SDS) for the determination of the total phenolic content (TPC) in juices were critically evaluated in terms of their selectivity. The evaluation was carried out using 86 analytes, including 22 phenolic compounds (phenolic acids and polyphenols), 6 known non-phenolic antioxidants, 9 amino acids and a number of proteins, carbohydrates, nucleotide bases, inorganic salts and other compounds. Each method was sensitive toward phenolic compounds (PCs). However, the KMnO4-COH CL system showed a higher sensitivity toward phenolic acids and also responded to non-phenolic antioxidants. The other two systems showed higher sensitivity toward polyphenolic compounds than to phenolic acids and did not responded to all other compounds including non-phenolic antioxidants. PMID:27507524

  13. High-speed fuel tracer fluorescence and OH radical chemiluminescence imaging in a spark-ignition direct-injection engine.

    Science.gov (United States)

    Smith, James D; Sick, Volker

    2005-11-01

    An innovative technique has been demonstrated to achieve crank-angle-resolved planar laser-induced fluorescence (PLIF) of fuel followed by OH* chemiluminescence imaging in a firing direct-injected spark-ignition engine. This study used two standard KrF excimer lasers to excite toluene for tracking fuel distribution. The intensified camera system was operated at single crank-angle resolution at 2000 revolutions per minute (RPM) for 500 consecutive cycles. Through this work, it has been demonstrated that toluene and OH* can be imaged through the same optical setup while similar signal levels are obtained from both species, even at these high rates. The technique is useful for studying correlations between fuel distribution and subsequent ignition and flame propagation without the limitations of phase-averaging imaging approaches. This technique is illustrated for the effect of exhaust gas recirculation on combustion and will be useful for studies of misfire causes. Finally, a few general observations are presented as to the effect of preignition fuel distribution on subsequent combustion.

  14. Flow—injection Chemiluminescence Determination of Reserpine in Medicine and Biological Fluids with Controlled—Reagent—Release Technology

    Institute of Scientific and Technical Information of China (English)

    宋正华; 张尼

    2003-01-01

    A sensitive and rapid chemiluminescence(CL) flow injection with controlled-reagent-release technology for the determination of reserpine was proposed.The Cl reagents,luminol and dichromate,uses in this sensor,were all immobilized on anion-exchange resin.Through injection of 100μl of water,the reagents on the anion-ex-change resin column were eluted and in the presence of reserpine ,the CL intensity was decreased,by which reserpine could be sensed.Reserpine was quantified by measuring the decrement of CL intensity,which was observed linear with the logrithm of reserpine concentration in the rage of 1.0-500.0ng/mL,and the limit of detection was 0.4ng/mL(3σ)with a relative standard deviation of less than 3.0?The proposed procedure was applied in the assay of reserpine in pharmaceutical preparation and biological fhuids without any pre-treatment process and with sampling frequencies of 72 times per hour.

  15. Application of a novel co-enzyme reactor in chemiluminescence flow-through biosensor for determination of lactose.

    Science.gov (United States)

    Yang, Chunyan; Zhang, Zhujun; Shi, Zuolong; Xue, Pan; Chang, Pingping; Yan, Ruifang

    2010-06-30

    A novel enzyme reactor with co-immobilization of beta-galactosidase and glucose oxidase in calcium alginate fiber (CAF) and amine modified nanosized mesoporous silica (AMNMS) was prepared which incorporate the adsorption and catalysis of AMNMS with the cage effect of the polymer to increase catalytic activity and stability of immobilized enzyme. The enzyme reactor was applied to prepare a chemiluminescence (CL) flow-through biosensor for determination of lactose combined with a novel luminol-diperiodatonickelate (DPN) CL system we reported. It shows that the CL flow-through biosensor possesses long lifetime, high stability, high catalytic activity and sensitivity. The relative CL intensity was linear with the lactose concentration in the range of 8 x 10(-8) - 4 x 10(-6) g mL(-1) with the detection limit of 2.7 x 10(-8) g mL(-1) (3sigma). It has been successfully applied to the determination of lactose in milk. PMID:20685473

  16. A novel system of galangin-potassium permanganate-polyphosphoric acid for the determination of tryptophan and its chemiluminescence mechanism.

    Science.gov (United States)

    Li, Li; Guo, Ruibin; Zhang, Dongxia; Du, Xinzhen

    2015-08-01

    A novel galangin-potassium permanganate (KMnO4)-polyphosphoric acid (PPA) system was found to have an outstanding response to tryptophan (Trp). Trp determination using this KMnO4 -PPA system was enhanced significantly in the presence of galangin. A highly sensitive flow-injection chemiluminescence (CL) method to determine Trp was developed based on the CL reaction of galangin-KMnO4 -Trp in PPA media. The presence of galangin, a member of the flavonol class of flavonoid complexes, greatly increased the luminous intensity of Trp in KMnO4 -PPA systems. Under optimized conditions, Trp was determined in the 0.05-10 µg/mL range, with a detection limit (3σ) of 5.0 × 10(-3)  µg/mL. The relative standard deviation (RSD) was 1.0% for 11 replicate determinations of 1.0 µg/mL Trp. Two synthetic samples were determined selectively with recoveries of 98.4-100.1% in the presence of other amino acids. The possible mechanism is summarized as follows: excited states of Mn(II)(*) and Mn(III(*) types are the main means of generating chemical luminescent species, and Trp concentration and luminescence intensity have a linear relationship, which enables quantitative analysis. PMID:25271024

  17. In vitro inhibitory effects of Moringa oleifera leaf extract and its major components on chemiluminescence and chemotactic activity of phagocytes.

    Science.gov (United States)

    Vongsak, Boonyadist; Gritsanapan, Wandee; Wongkrajang, Yuvadee; Jantan, Ibrahim

    2013-11-01

    The ethanol extract of Moringa oleifera Lam. leaves and its major constituents, crypto-chlorogenic acid, quercetin 3-O-glucoside and kaempferol 3-O-glucoside, were investigated on the respiratory burst of human whole blood and isolated human polymorphonuclear leukocytes (PMNs) using a luminol-based chemiluminescence assay. The chemotactic migration of PMNs was also investigated using the Boyden chamber technique. The ethanol extract demonstrated inhibitory activities on the oxidative burst and the chemotactic migration of PMNs. Quercetin 3-O-glucoside, crypto-chlorogenic acid, and kaempferol 3-O-glucoside, isolated from the extract, expressed relatively strong inhibitory activity on the oxidative burst of PMNs with IC50 values of 4.1, 6.7 and 7.0 microM, respectively, comparable with that of aspirin. They also demonstrated strong inhibition of chemotatic migration of PMNs with IC50 values of 9.5, 15.9 and 18.2 microM, respectively. The results suggest that M. oleifera leaves could modulate the immune response of human phagocytes, linking to its ethnopharmacological use as an anti-inflammatory agent. The immunomodulating activity of the plant was mainly due to its major components.

  18. Long-lasting chemiluminescence of luminol on electrochemically pre-oxidized platinum electrodes in NaOH solution.

    Science.gov (United States)

    Lin, X Q; Sun, Y G; Cui, H

    2000-01-01

    A long-lasting bright chemiluminescence (CL) of luminol was generated at polycrystalline platinum electrodes with open circuit. The CL can last for several hours with the presence of O(2) in the solution when the electrode was preoxidized at potentials more positive than 1.10 V vs. SCE. The effects of the varieties of solution conditions and surface states of the electrode on the CL intensity and the interfacial potential of the electrode were investigated. It was proposed that PtO was generated at the pre-oxidized potentials and played a role of catalyst of luminol oxidation for generating the CL. The redox couple of PtO/Pt(active) at the electrode surface was maintained in the presence of O(2) and luminol, and generated the interfacial potential more positive than 140 mV. Mathematical treatment of the reaction mechanism was conducted, which led to an approximated expression of a steady CL intensity (I(CL)) as a function of the pre-polarization potential (E( h)) and time (tau( h)) of the electrode. An empirical equation, (I(CL))(4/3) = 3480(-1 + 0.82E( h) + 0.037 ln tau( h)), was estimated from the experimental data. PMID:11038487

  19. Flow injection chemiluminescence immunoassay based on resin beads, enzymatic amplification and a novel monoclonal antibody for determination of Hg(2+).

    Science.gov (United States)

    Xu, Mingxia; Chen, Mengting; Dong, Tiantian; Zhao, Kang; Deng, Anping; Li, Jianguo

    2015-09-21

    In the present work, a simple and sensitive flow injection chemiluminescent competitive immunoassay was developed for the determination of mercury(II) ion (Hg(2+)) based on carboxylic resin beads, a novel specific monoclonal antibody (McAb) and HRP enzyme-amplification. Resin beads with carboxyl groups were creatively employed as supports for immobilizing more coating antigen through acylamide bonds. With a competitive-type assay mode, the Hg(2+) in solution competed with the immobilized coating antigen for the limited McAb. Then, the second antibody labeled with HRP was introduced, and an effectively increased CL was obtained, which was ascribed to the catalytic activity of HRP for the luminol-PIP-H2O2 reaction. With increasing concentration of Hg(2+), the CL of this system decreases because less HRP is present in the CL reaction. At optimal conditions, the CL signal displayed a good linear relation toward Hg(2+) in the range of 0.05-200 ng mL(-1) with a detection limit (3σ) of 0.015 ng mL(-1). The immunosensor possessed high specificity, acceptable accuracy and reproducibility, and was examined in real samples with favorable results. This immunoassay will have intriguing application prospects for the determination of other heavy metal ions and environmental contaminants.

  20. New enzyme-linked chemiluminescent immunosorbent digoxin assay is free from interference of Chinese medicine DanShen.

    Science.gov (United States)

    Dasgupta, Amitava; Kang, Edward; Olsen, Margaret; Actor, Jeffrey K; Datta, Pradip

    2006-12-01

    DanShen is a traditional Chinese medicine indicated for cardiovascular diseases. The potential interference of DanShen with serum digoxin measurement was investigated using a new enzyme-linked chemiluminescent immunosorbent (ECLIA) digoxin assay. Aliquots of drug-free serum were supplemented with ethyl acetate extract of DanShen (4 different brands studied), and apparent digoxin concentrations were measured by the ECLIA as well as fluorescence polarization immunoassay (FPIA) and a turbidimetric assay for comparison. Mice were also fed 4 DanShen preparations and apparent digoxin concentrations were subsequently measured. In another experiment, serum pools containing digoxin were further supplemented with DanShen extracts and digoxin concentrations were measured again by all 3 assays. No apparent digoxin concentration was observed when aliquots of drug-free serum pools were supplemented with DanShen and digoxin concentrations were measured by the ECLIA or the turbidimetric assay. In contrast, significant apparent digoxin concentrations were observed using FPIA, and the highest apparent digoxin concentration was observed with brand 4 of DanShen extract. Similarly, when mice were fed with this herb, significant apparent digoxin concentrations were also observed using FPIA, but neither ECLIA nor turbidimetric assay showed any apparent digoxin concentration. When aliquots of digoxin pool were further supplemented with various DanShen extract, the apparent digoxin concentrations were significantly increased when FPIA was used. In contrast, digoxin concentrations in the presence of DanShen extract compared well with the digoxin concentration of the original pool when ECLIA or turbidimetric assay was used. We conclude that DanShen does not interfere with serum digoxin measurement using a more recently released ECLIA digoxin assay. PMID:17164693

  1. Combining complement fixation and luminol chemiluminescence for ultrasensitive detection of avian influenza A rH7N9.

    Science.gov (United States)

    Li, Man; Shi, ZhuanZhuan; Li, ChangMing; Yu, Ling

    2016-03-21

    The complement fixation test (CFT) is a serological test that can be used to detect the presence of either a specific antibody or antigen to diagnose infections. In a conventional CFT, the assay result is determined by observing the clarity of the reaction solution or the sediment of red cells by the naked eye. Although the assay conditions are thereafter simplified, the sensitivity of the assay would be sacrificed due to the limitation of bulk observation. Inspired by the forensic scientists to examine blood at the scene of the crime, we rationally argued that the luminol chemiluminescence (CL) reaction could be applied in the CFT to sense physiological complement-mediated haemolytic phenomena for sensitive protein detection. The combination of the CFT and the luminol CL system was demonstrated in detection of rH7N9, a recombinant avian influenza virus protein. The testing can be accomplished within 2.5 h and the linear detection range covers 0.25 fg mL(-1) to 25 ng mL(-1). The feasibility of the CL based CFT in assaying a real biopsy was successfully demonstrated by specifically detecting rH7N9 and the carcinoembryonic antigen (CEA) in human serum. This new type of protein detection approach inherits the beauty of complement-mediated assay, such as being fast, and no protein immobilization, blocking and washing. In addition, the participation of luminol CL enables us to quantitatively analyse the intensity of a haemeolysis process, ameliorating the limitation of bulk observation in traditional CFT. It is anticipated that the luminol CL-CFT assay would be particularly suitable for investigation of small molecules, toxins, and short peptides. PMID:26863640

  2. PMMA microreactor for chemiluminescence detection of Cu (II) based on 1,10-Phenanthroline-hydrogen peroxide reaction.

    Science.gov (United States)

    Chen, Xueye; Shen, Jienan; Li, Tiechuan

    2016-01-01

    A microreactor for the chemiluminescence detection of copper (II) in water samples, based on the measurement of light emitted from the copper (II) catalysed oxidation of 1,10-phenanthroline by hydrogen peroxide in basic aqueous solution, is presented. Polymethyl methacrylate (PMMA) was chose as material for fabricating the microreactor with mill and hot bonding method. Optimized reagents conditions were found to be 6.3 × 10(-5)mol/L 1,10-phenanthroline, 1.5 × 10(-3)mol/L hydrogen peroxide, 7.0 × 10(-2)mol/L sodium hydroxide and 2.4 × 10(-5)mol/L Hexadecyl trimethyl ammonium Bromide (CTMAB). In the continuous flow injection mode the system can perform fully automated detection with a reagent consumption of only 3.5 μL each time. The linear range of the Cu (II) ions concentration was 1.5 × 10(-8) mol/L to 1.0 × 10(-4) mol/L, and the detection limit was 9.4 × 10(-9)mol/L with the S/N ratio of 4. The relative standard deviation was 3.0 % for 2.0 × 10(-6) mol/L Cu (II) ions (n = 10). The most obvious features of the detection method are simplicity, rapidity and easy fabrication of the microreactor. PMID:26788016

  3. A cascade amplification strategy based on rolling circle amplification and hydroxylamine amplified gold nanoparticles enables chemiluminescence detection of adenosine triphosphate.

    Science.gov (United States)

    Wang, Ping; Zhang, Tonghuan; Yang, Taoyi; Jin, Nan; Zhao, Yanjun; Fan, Aiping

    2014-08-01

    A highly sensitive and selective chemiluminescent (CL) biosensor for adenosine triphosphate (ATP) was developed by taking advantage of the ATP-dependent enzymatic reaction (ATP-DER), the powerful signal amplification capability of rolling circle amplification (RCA), and hydroxylamine-amplified gold nanoparticles (Au NPs). The strategy relies on the ability of ATP, a cofactor of T4 DNA ligase, to trigger the ligation-RCA reaction. In the presence of ATP, the T4 DNA ligase catalyzes the ligation reaction between the two ends of the padlock probe, producing a closed circular DNA template that initiates the RCA reaction with phi29 DNA polymerase and dNTP. Therein, many complementary copies of the circular template can be generated. The ATP-DER is eventually converted into a detectable CL signal after a series of processes, including gold probe hybridization, hydroxylamine amplification, and oxidative gold metal dissolution coupled with a simple and sensitive luminol CL reaction. The CL signal is directly proportional to the ATP level. The results showed that the detection limit of the assay is 100 pM of ATP, which compares favorably with those of other ATP detection techniques. In addition, by taking advantage of ATP-DER, the proposed CL sensing system exhibits extraordinary specificity towards ATP and could distinguish the target molecule ATP from its analogues. The proposed method provides a new and versatile platform for the design of novel DNA ligation reaction-based CL sensing systems for other cofactors. This novel ATP-DER based CL sensing system may find wide applications in clinical diagnosis as well as in environmental and biomedical fields.

  4. Development and optimization of an integrated capillary-based opto-microfluidic device for chemiluminescence quantitative detection

    International Nuclear Information System (INIS)

    A capillary-action driven device amenable for integration of organic photodiodes (OPDs) was developed for monitoring parallel chemiluminescence (CL) reactions. Device characterization was conducted using finite element method (FEM) simulations. Definition of the simulation setup, dimensional optimization of the reaction chamber and overall geometrical characterization of the microfluidic device were the main simulation results. Furthermore, a non-uniform filling process was observed during the final simulation of the capillary device. Validation of this result and the proposed capillary-driven filling process was later confirmed by experimental results. Experimental testing performed on a single chamber defined an optimal exposure time to the luminescent substrate of 5 min, indicating a quick analyte detection time. Further tests using one chamber presented a linear relation between the signal-to-noise ratio and increasing concentrations of the protein used. A measured limit of detection of 28 nM was obtained for streptavidin. Regarding the tests performed on the whole device, acceptable values of 39 s ± 5 s were obtained for the luminescent substrate total filling times. Also, the microfluidic device showed the capability to perform a quantitative detection of the occurring CL reactions. Weaker optical signals, due to the occurrence of CL reactions, were detected in the chambers with a later filling process, as predicted by simulation results. Notwithstanding these results, the capillary-based device is promising for quantitative detection of proteins in future point-of-care systems, presenting an unprompted filling process and parallel quantitative detection capability. (paper)

  5. Determination of total sulfur compounds and benzothiazole in asphalt fume samples by gas chromatography with sulfur chemiluminescence detection.

    Science.gov (United States)

    Jaycox, L B; Olsen, L D

    2000-09-01

    As part of a collaborative project between the National Institute for Occupational Safety and Health and the Federal Highway Administration to evaluate asphalt pavers' exposures to asphalt fume and their potential health effects, a method was developed for the determination of total sulfur compounds and benzothiazole in asphalt fume samples. Asphalt fume samples were collected from asphalt mixtures with and without the addition of ground-up rubber tires. The asphalt fume samples were collected with sampling trains that consisted of a Teflon membrane filter and an XAD-2 adsorbent tube. Filter and sampling tube media were extracted with hexane and subsequently analyzed by gas chromatography with a sulfur chemiluminescence detector. Separation was achieved with a 100 percent dimethyl polysiloxane fused silica column. Typical calibration curves had linear correlation coefficients of 0.99 or better with a relative standard deviation (RSD) of 5 percent. Benzothiazole desorption efficiency (DE) determined using spiked sampling tubes ranged from 96.5 percent at 5.0 micrograms to 89.4 percent at 40 micrograms with RSD values from 0.9 to 4.0 percent. Benzothiazole storage recovery determined using sampling tubes spiked at 20 micrograms and refrigerated for 30 days at 4 degrees C was 89.8 percent when corrected for the DE with an RSD of 1.1 percent. The limit of detection for the method determined using spiked sampling tubes was 0.30 microgram. Quantitation for total sulfur compounds and benzothiazole was against benzothiazole standards in hexane. Because of detector selectivity, sample preparation consisted of a simple hexane extraction even when samples had a high background due to hydrocarbon overload. Detector sensitivity provided quantitation in the sub-microgram region. Because of the sample preparation step and because benzothiazole was determined during the same analysis run, this method is straightforward and analytically efficient. The method has been used to

  6. Simultaneous detection of forbidden chemical residues in milk using dual-label time-resolved reverse competitive chemiluminescent immunoassay based on amine group functionalized surface.

    Directory of Open Access Journals (Sweden)

    Dongdong Zhang

    Full Text Available In this study, a sensitive dual-label time-resolved reverse competitive chemiluminescent immunoassay was developed for simultaneous detection of chloramphenicol (CAP and clenbuterol (CLE in milk. The strategy was performed based on the distinction of the kinetic characteristics of horseradish peroxidase (HRP and alkaline phosphatase (ALP in chemiluminesecence (CL systems and different orders of magnitude in HRP CL value for CAP and ALP CL value for CLE in the chemiluminescent immunoassay. Capture antibodies were covalently bound to the amine group functionalized chemiluminescent microtiter plate (MTP for efficient binding of detection antibodies for the enzymes labeled CAP (HRP-CAP and CLE (ALP-CLE. The CL signals were recorded at different time points by the automatic luminometers with significant distinction in the dynamic curves. When we considered the ALP CL value (about 10(5 of CLE as background for HRP CL signal value (about 10(7 of CAP, there was no interaction from ALP CL background of CLE and the differentiation of CAP and CLE can be easily achieved. The 50% inhibition concentration (IC50 values of CAP and CLE in milk samples were 0.00501 µg L(-1 and 0.0128 µg L(-1, with the ranges from 0.0003 µg L(-1 to 0.0912 µg L(-1 and from 0.00385 µg L(-1 to 0.125 µg L(-1, respectively. The developed method is more sensitive and of less duration than the commercial ELISA kits, suitable for simultaneous screening of CAP and CLE.

  7. Rational design for enhancing inflammation-responsive in vivo chemiluminescence via nanophotonic energy relay to near-infrared AIE-active conjugated polymer.

    Science.gov (United States)

    Seo, Young Hun; Singh, Ajay; Cho, Hong-Jun; Kim, Youngsun; Heo, Jeongyun; Lim, Chang-Keun; Park, Soo Young; Jang, Woo-Dong; Kim, Sehoon

    2016-04-01

    H2O2-specific peroxalate chemiluminescence is recognized as a potential signal for sensitive in vivo imaging of inflammation but the effect of underlying peroxalate-emitter energetics on its efficiency has rarely been understood. Here we report a simple nanophotonic way of boosting near-infrared chemiluminescence with no need of complicated structural design and synthesis of an energetically favored emitter. The signal enhancement was attained from the construction of a nanoparticle imaging probe (∼26 nm in size) by dense nanointegration of multiple molecules possessing unique photonic features, i.e., i) a peroxalate as a chemical fuel generating electronic excitation energy in response to inflammatory H2O2, ii) a low-bandgap conjugated polymer as a bright near-infrared emitter showing aggregation-induced emission (AIE), and iii) an energy gap-bridging photonic molecule that relays the chemically generated excitation energy to the emitter for its efficient excitation. From static and kinetic spectroscopic studies, a green-emissive BODIPY dye has proven to be an efficient relay molecule to bridge the energy gap between the AIE polymer and the chemically generated excited intermediate of H2O2-reacted peroxalates. The energy-relayed nanointegration of AIE polymer and peroxalate in water showed a 50-times boosted sensing signal compared to their dissolved mixture in THF. Besides the high H2O2 detectability down to 10(-9) M, the boosted chemiluminescence presented a fairly high tissue penetration depth (>12 mm) in an ex vivo condition, which enabled deep imaging of inflammatory H2O2 in a hair-covered mouse model of peritonitis. PMID:26826300

  8. Application of a Newly Developed High-Sensitivity HBsAg Chemiluminescent Enzyme Immunoassay for Hepatitis B Patients with HBsAg Seroclearance

    OpenAIRE

    Shinkai, Noboru; Matsuura, Kentaro; Sugauchi, Fuminaka; Watanabe, Tsunamasa; Murakami, Shuko; Iio, Etsuko; Ogawa, Shintaro; Nojiri, Shunsuke; Joh, Takashi; Tanaka, Yasuhito

    2013-01-01

    We modified and automated a highly sensitive chemiluminescent enzyme immunoassay (CLEIA) for surface antigen (HBsAg) detection using a combination of monoclonal antibodies, each for a specific epitope of HBsAg, and by improving an earlier conjugation technique. Of 471 hepatitis B virus (HBV) carriers seen in our hospital between 2009 and 2012, 26 were HBsAg seronegative as determined by the Abbott Architect assay. The Lumipulse HBsAg-HQ assay was used to recheck those 26 patients who demonstr...

  9. Electrogenerated chemiluminescence sensor for the determination of metoclopramide using ordered mesoporous carbon for immobilizing tris(2,2 -bipyridyl)ruthenium

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    A novel electrogenerated chemiluminescence(ECL)sensor for the determination of metoclopramide was developed by employing ruthenium complex as an ECL signal producer and an ordered mesoporous carbon(OMC)material as modified material.The ECL sensor was fabricated by adsorption ruthenium complex into a mixture of OMC and Nafion,which showed good electrochemical and ECL behaviors.It was found that the ECL intensity of the sensor fabricated was greatly enhanced in the presence of metoclopramide.Based on this fin...

  10. Detection of pyrrolizidine alkaloids using flow analysis with both acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence

    International Nuclear Information System (INIS)

    For the first time, analytically useful chemiluminescence was elicited from the reactions of the pyrrolizidine alkaloids. Heliotrine, retronecine, supinine, monocrotaline and echinatine N-oxide yielded chemiluminescence upon reaction with tris(2,2'-bipyridyl)ruthenium(II) whilst lasiocarpine, its N-oxide and supinine elicited light upon reaction with acidic potassium permanganate. Detection limits for heliotrine were 1.25 x 10-7 M and 9 x 10-9 M for tris(2,2'-bipyridyl)ruthenium(III) perchlorate with flow injection analysis (FIA) and the silica-immobilised reagent (4-[4-(dichloromethylsilanyl)-butyl]-4'-methyl-2,2'-bipyridine)bis (2,2'-bipyridyl)ruthenium(II) with sequential injection analysis (SIA), respectively. Lasiocarpine was detectable at 1.4 x 10-7 M using acidic potassium permanganate with FIA. Additionally, the silica-immobilised reagent was optimised with respect to the oxidant (ammonium ceric nitrate) concentration and the aspiration times which afforded a detection limit for codeine of 5 x 10-10 M using SIA

  11. Highly sensitive trivalent copper chelate–luminol chemiluminescence system for capillary electrophoresis chiral separation and determination of ofloxacin enantiomers in urine samples

    Directory of Open Access Journals (Sweden)

    Hao-Yue Xie

    2014-12-01

    Full Text Available A simple, fast and sensitive capillary electrophoresis (CE strategy combined with chemiluminescence (CL detection for analysis of ofloxacin (OF enantiomers was established in the present work. Sulfonated β-cyclodextrin (β-CD was used as the chiral additive being added into the running buffer of luminol–diperiodatocuprate (III (K5[Cu(HIO62], DPC chemiluminescence system. Under the optimum conditions, the proposed method was successfully applied to separation and analysis of OF enantiomers with the detection limits (S/N=3 of 8.0 nM and 7.0 nM for levofloxacin and dextrofloxacin, respectively. The linear ranges were both 0.010–100 μM. The method was utilized for analyzing OF in urine; the results obtained were satisfactory and recoveries were 89.5–110.8%, which demonstrated the reliability of this method. This approach can also be further extended to analyze different commercial OF medicines.

  12. QM/MM study on the light emitters of aequorin chemiluminescence, bioluminescence, and fluorescence: a general understanding of the bioluminescence of several marine organisms.

    Science.gov (United States)

    Chen, Shu-Feng; Ferré, Nicolas; Liu, Ya-Jun

    2013-06-24

    Aequorea victoria is a type of jellyfish that is known by its famous protein, green fluorescent protein (GFP), which has been widely used as a probe in many fields. Aequorea has another important protein, aequorin, which is one of the members of the EF-hand calcium-binding protein family. Aequorin has been used for intracellular calcium measurements for three decades, but its bioluminescence mechanism remains largely unknown. One of the important reasons is the lack of clear and reliable knowledge about the light emitters, which are complex. Several neutral and anionic forms exist in chemiexcited, bioluminescent, and fluorescent states and are connected with the H-bond network of the binding cavity in the protein. We first theoretically investigated aequorin chemiluminescence, bioluminescence, and fluorescence in real proteins by performing hybrid quantum mechanics and molecular mechanics methods combined with a molecular dynamics method. For the first time, this study reported the origin and clear differences in the chemiluminescence, bioluminescence and fluorescence of aequorin, which is important for understanding the bioluminescence not only of jellyfish, but also of many other marine organisms (that have the same coelenterazine caved in different coelenterazine-type luciferases).

  13. A novel chemiluminescence method for determination of bisphenol Abased on the carbon dot-enhanced HCO3−–H2O2 system

    International Nuclear Information System (INIS)

    A simple and sensitive chemiluminescence (CL) method on the basis of carbon dot (C-dot) enhanced HCO3−–H2O2 system, is designed for the determination of bisphenol A (BPA). The very weak CL of the HCO3−–H2O2 system is enhanced by a factor of ∼100 in the presence of C-dots. Possible mechanisms that lead to the effect were elucidated by recording fluorescence and CL spectra and studying the effect of some radical scavengers. This enhancement is inhibited by BPA in the concentration range from 1.0 to 100 µg L−1. This is exploited for its trace determination with a detection limit (3 s) of 0.3 µg L−1. The established method was applied to the determination of BPA in baby bottle and water samples with satisfactory results. - Highlights: • The effect of carbon dots on HCO3−–H2O2 chemiluminescence reaction is studied. • Carbon dots greatly enhance the CL signal of this reaction (∼100 fold). • The new CL system was applied to determination of bisphenol A in real samples

  14. Electrogenerated chemiluminescence reaction of tris(2,2'-bipyridine)ruthenium(II) with 2,5-dimethylthiophene as co-reactant in aqueous solution

    International Nuclear Information System (INIS)

    A novel effective co-reactant for electrogenerated chemiluminescence (ECL) of Ru(bpy)32+ has been found. α-Position-dialkylated thiophene derivatives such as 2,5-dimethylthiophene (DMT) could be used as a co-reactant for Ru(bpy)32+ ECL. The reaction mechanism of the Ru(bpy)32+/DMT system was proposed on the basis of the identification of the reaction product, the relationship between the molecular structure and the chemiluminescent intensity, and the electrochemical study. The obtained reaction mechanism was similar to that of the Ru(bpy)32+/aliphatic tertiary amine system. Based on these results, the preliminary studies of the Ru(bpy)32+ ECL detection system using DMT as a co-reactant were performed. Under the optimal ECL conditions, the plot of ECL intensity versus the concentration of Ru(bpy)32+ was linear over the concentration range 1.0 x 10-8 to 1.5 x 10-7 M (determination coefficient = 0.9996)

  15. Chemiluminescent determination of vanadium(IV) using a cinchomeronic hydrazide-H{sub 2}O{sub 2} system and flow injection analysis

    Energy Technology Data Exchange (ETDEWEB)

    Pradana Perez, J.A. [Departamento de Ciencias Analiticas, Facultad de Ciencias, Universidad Nacional de Educacion a Distancia, c/Senda del Rey 9, 28040 Madrid (Spain); Alegria, J.S. Durand [Departamento de Ciencias Analiticas, Facultad de Ciencias, Universidad Nacional de Educacion a Distancia, c/Senda del Rey 9, 28040 Madrid (Spain); Hernando, P. Fernandez [Departamento de Ciencias Analiticas, Facultad de Ciencias, Universidad Nacional de Educacion a Distancia, c/Senda del Rey 9, 28040 Madrid (Spain)]. E-mail: pfhernando@ccia.uned.es; Sierra, A. Narros [Departamento de Ingenieria Quimica y del Medio Ambiente, Escuela Tecnica Superior de Ingenieros Industriales, Universidad Politecnica de Madrid, Madrid (Spain)

    2005-04-22

    This paper proposes a new chemiluminescent flow injection analysis (FIA) method for the determination of vanadium(IV) ions in aqueous media. The method is based on the chemiluminescent reaction that occurs between cinchomeronic hydrazide (CH) and hydrogen peroxide in a strongly alkaline medium, in which vanadium(IV) acts as a catalyst. The chemical and physical variables involved in the flow injection system are optimised using a modified simplex method. Vanadium ions can be detected in the 0.08 and 1.00 {mu}g mL{sup -1} range; the detection limit for a signal-to-noise ratio of 3 is 0.08 {mu}g mL{sup -1}. Great variations in the quantum yield were observed when cobalt(II), chromium(III), copper(II) and/or nickel(II) were present in the reaction medium. The proposed method is selective and simple, and can be successfully used to analyse water samples without the need for separation or preconcentration processes.

  16. Electrogenerated chemiluminescence quenching of Ru(bpy){sub 3} {sup 2+} (bpy=2,2 Prime -bipyridine) in the presence of acetaminophen, salicylic acid and their metabolites

    Energy Technology Data Exchange (ETDEWEB)

    Haslag, Catherine S. [Department of Chemistry, Missouri State University, Springfield, Missouri 65897 (United States); Richter, Mark M., E-mail: MarkRichter@missouristate.edu [Department of Chemistry, Missouri State University, Springfield, Missouri 65897 (United States)

    2012-03-15

    Quenching of Ru(bpy) {sub 3}{sup 2+} (bpy=2,2 Prime -bipyridine) coreactant electrogenerated chemiluminescence (ECL) has been observed in the presence of acetaminophen, salicylic acid and related complexes. However, no quenching is observed with the acetylsalicylic acid. In most instances, quenching is observed with 100-fold excess of quencher (compared to ECL luminophore) with complete quenching observed between 10,000 and 100,000 fold excess. Fluorescence and UV-vis experiments coupled with bulk electrolysis support the formation of benzoquinone products upon electrochemical oxidation. The mechanism of quenching may involve the interaction of the electrochemically generated benzoquinone species with (i) the {sup Low-Asterisk }Ru(bpy){sub 3}{sup 2+} excited state or (ii) highly energetic coreactant radicals. - Highlights: Black-Right-Pointing-Triangle Efficient quenching of the electrogenerated chemiluminescence is observed. Black-Right-Pointing-Triangle Acetaminophen, salicylic acid and related compounds can be detected. Black-Right-Pointing-Triangle The mechanism of quenching involves benzoquinones formed upon electrolysis.

  17. Chemiluminescence system for direct determination and mapping of ultra-trace metal impurities on a silicon wafer.

    Science.gov (United States)

    Kim, Romertta; Sung, Y I; Lee, J S; Lim, H B

    2010-11-01

    A highly sensitive chemiluminescence (CL) system which consumed low sample and reagent volumes in the microlitre range was developed for direct determination and mapping of ultra-trace metal contaminants on solid surfaces, such as silicon wafers or flat display panels. The analytical result of the system was confirmed with ICP-MS. The system was composed of a scanner, sensor and a wafer moving stage. The scanner, with a scanning tip made of 0.03'' i.d. PTFE tubing, was used to collect metal impurities on the wafer surface with 5 μL of scanning solution. A coaxial sensing head of about 13 mm o.d. and 110 mm height was designed both to inject a luminescent reagent of luminol-H(2)O(2) mixture and to collect the luminescence light resulting from the reaction with metal ions of Co(2+), Fe(2+), Cu(2+), and Ni(2+). Due to the almost zero background, an extremely low limit of detection of 20.8 pg/mL for Co(2+) in 1% hydrofluoric acid (HF) was obtained from the calibration curve. In order to map the spatial distribution of the impurities, 11 cross sections of a Co-contaminated wafer were selected and scanned individually with a diluted HF solution. A contaminant level of 1.45-7.11 × 10(11) atoms cm(-2) was obtained for each section with an average of 4.21 × 10(11) atoms cm(-2), which was similar to the analytical result of 5.48 × 10(11) atoms cm(-2) obtained from vapor phase deposition-inductively coupled plasma-mass spectrometry (VPD-ICP-MS). Although this CL system does not have selectivity for each specific metal ion, its high sensitivity facilitates the monitoring and mapping of metal impurities of Co, Fe, Cu, etc. on the wafer directly and it can be used as an on-line inspection sensor for the first time in the semiconductor industry.

  18. Comparative studies of the chemiluminescent horseradish peroxidase-catalyzed peroxidation of acridan (GZ-11) and luminol reactions: Effect of pH and scavengers of reactive oxygen species on the light intensity of these systems

    NARCIS (Netherlands)

    Osman, A.M.; Zomer, G.; Laane, C.; Hilhorst, R.

    2000-01-01

    In this study, the chemiluminescent horseradish peroxidase/H2O2-catalysed oxidation of acridan (GZ-11) substrate was compared with the well-characterized light-producing luminol reaction. p-Iodophenol and p-phenylphenol were used as enhancers, respectively, for the luminol and acridan reactions. The

  19. Determination of Montelukast in Plasma Using β - Cyclodextrins Coated on CoFe2O4 Magnetic Nanoparticles in Luminol-H2O2 Chemiluminescence System Optimized by Doehlert Design.

    Science.gov (United States)

    Samadi-Maybodi, Abdolraouf; Bakhtiar, Alireza; Fatemi, Mohammad Hossein

    2016-05-01

    A novel chemiluminescence method using β - cyclodextrins coated on CoFe2O4 magnetic nanoparticles is proposed for the chemiluminometric determination of montelukast in plasma. The effect of coated β - cyclodexterinon CoFe2O4 magnetic nanoparticles in the chemiluminescence of luminol-H2O2 system was investigated. It was found that β - cyclodexterin coated on CoFe2O4 magnetic nanoparticles could greatly enhance the chemiluminescence of the luminol-H2O2 system. Doehlert design was applied in order to optimize the number of experiments to be carried out to ascertain the possible interactions between the parameters and their effects on the chemiluminescence emission intensity. This design was selected because the levels of each variable may vary in a very efficient way with few experiments. Doehlert design and response surface methodology have been employed for optimization pH and concentrations of the components. Results showed under the optimized experimental conditions, the relative CL intensity (ΔI) is increased linearly in the concentration range of 0.003-0.586 μgml(-1) of montelukast with limit of detection (LOD) 1.09 × 10(-4) μgml(-1) at S/N ratio of 3, limit of quantitative (LOQ) 3.59 × 10(-4) μgml(-1) and the relative standard deviation 2.63 %. The method has been successfully applied to the determination of montelukast in plasma of human body. Results specified that relative chemiluminescence intensity (ΔI) has good proportional with the montelukast concentration with R(2) = 0.99979. The test of the recovery efficiency for known amounts of montelukast was also performed, the recoveries range obtained from 98.2 to 103.3 %, with RSDs of <4 % indicated that the proposed method was reliable. PMID:26979057

  20. Scanning electrochemical microscopy study of ion annihilation electrogenerated chemiluminescence of rubrene and [Ru(bpy)3]2+.

    Science.gov (United States)

    Rodríguez-López, Joaquín; Shen, Mei; Nepomnyashchii, Alexander B; Bard, Allen J

    2012-06-01

    Scanning electrochemical microscopy (SECM) was used for the study of electrogenerated chemiluminescence (ECL) in the radical annihilation mode. The concurrent steady-state generation of radical ions in the microgap formed between a SECM probe and a transparent microsubstrate provides a distance-dependent ECL signal that can provide information about the kinetics, stability, and mechanism of the light emission process. In the present study, the ECL emission from rubrene and [Ru(bpy)(3)](2+) was used to model the system by carrying out experiments with the SECM and light-detecting apparatus inside an inert atmosphere box. We studied the influence of the distance between the two electrodes, d, and the annihilation kinetics on the ECL light emission profiles under steady-state conditions, as well as the ECL profiles when carrying out cyclic voltammetry (CV) at a fixed d. Experimental results are compared to simulated results obtained through commercial finite element method software. The light produced by annihilation of the ions was a function of d; stronger light was observed at smaller d. The distance dependence of the ECL emission allows the construction of light approach curves in a similar fashion as with the tip currents in the feedback mode of SECM. These ECL approach curves provide an additional channel to describe the reaction kinetics that lead to ECL; good agreement was found between the ECL approach curve emission profile and the simulated results for a fast, diffusion-limited second-order annihilation process (k(ann) > 10(7) M(-1) s(-1)). In the CV mode at fixed distance, the ECL emission of rubrene showed two distinct signals at different potentials when fixing the substrate to generate the radical cation and scanning the tip to generate the radical anion. The first signal (pre-emission) corresponded to an emission well before reaching the generation of the radical anion and was more intense on Au than on Pt. The second ECL signal showed the expected

  1. A Chemiluminescence Optical Fiber Glucose Biosensor Based on Co-immobilizing Glucose Oxidase and Horseradish Peroxidase in a Sol-gel Film

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    An optical fiber bienzyme sensor based on the luminol chemiluminescent reaction was developed and demonstrated to be sensitive to glucose. Glucose oxidase(GOD) and horseradish peroxidase(HRP) were co-immobilized by microencapsulation in a sol-gel film derived from tetraethyl orthosilicate(TEOS). The calibration plots for glucose were established by the optical fiber glucose sensor fabricated by attaching the bienzyme silica gel onto the glass window of the fiber bundle. The linear range was 0.2-2 mmol/L and the detection limit was approximately 0.12 mmol/L. The relative standard deviation was 5.3% (n=6). The proposed biosensor was applied to glucose assay in ofloxacin injection successfully.

  2. Electrogenerated chemiluminescence of tris(2,2' bipyridine)ruthenium(II) using common biological buffers as co-reactant, pH buffer and supporting electrolyte.

    Science.gov (United States)

    Kebede, Noah; Francis, Paul S; Barbante, Gregory J; Hogan, Conor F

    2015-11-01

    A series of aliphatic tertiary amines (HEPES, POPSO, EPPS and BIS-TRIS) commonly used to buffer the pH in biological experiments, were examined as alternative, non-toxic co-reactants for the electrogenerated chemiluminescence (ECL) of tris(2,2'-bipyridine)ruthenium(ii) ([Ru(bpy)3](2+)). These were found to be very attractive as "multi-tasking" reagents, serving not only as co-reactants, but also fulfiling the roles of pH buffer and supporting electrolyte within an aqueous environment; thus significantly simplifying the overall ECL analysis. Sub-nanomolar detection limits were obtained for [Ru(bpy)3](2+) in the presence of BIS-TRIS, making this species an valuable option for co-reactant ECL-based bioanalytical applications. PMID:26347905

  3. Chemiluminescence determination of antioxidant property of Zizyphus mistol and Prosopis alba during oxidative stress generated in blood by Hemolytic Uremic Syndrome-producing Escherichia coli.

    Science.gov (United States)

    Albrecht, Claudia; Pellarin, María G; Baronetti, José; Rojas, María J; Albesa, Inés; Eraso, Alberto J

    2011-01-01

    This study was undertaken to elucidate the antioxidant effect of Zizyphus mistol and Prosopis alba, with the hypothesis that these fruits can counteract the induction of reactive oxygen species (ROS) caused by toxins produced by Escherichia coli. In the search of nutrients effective against the Hemolytic Uremic Syndrome (HUS), we detected by chemiluminescence a protective role of both plants, due to their natural antioxidants significantly decreasing the levels of ROS induced by toxins from E. coli in blood. The ferric reducing antioxidant power (FRAP) was found to be higher in Z. mistol than in P. alba. The chemical analyses of the phenols and flavonoids present in the fruit extracts indicated that the FRAP correlated with the amount of phenolic compounds, but not with the flavonoids analyzed. Both fruits studied reduce the induction of ROS, and in this way help to prevent the development of complications related to oxidative stress generated in the blood of patients with HUS.

  4. Electrogenerated chemiluminescence of tris(2,2' bipyridine)ruthenium(II) using common biological buffers as co-reactant, pH buffer and supporting electrolyte.

    Science.gov (United States)

    Kebede, Noah; Francis, Paul S; Barbante, Gregory J; Hogan, Conor F

    2015-11-01

    A series of aliphatic tertiary amines (HEPES, POPSO, EPPS and BIS-TRIS) commonly used to buffer the pH in biological experiments, were examined as alternative, non-toxic co-reactants for the electrogenerated chemiluminescence (ECL) of tris(2,2'-bipyridine)ruthenium(ii) ([Ru(bpy)3](2+)). These were found to be very attractive as "multi-tasking" reagents, serving not only as co-reactants, but also fulfiling the roles of pH buffer and supporting electrolyte within an aqueous environment; thus significantly simplifying the overall ECL analysis. Sub-nanomolar detection limits were obtained for [Ru(bpy)3](2+) in the presence of BIS-TRIS, making this species an valuable option for co-reactant ECL-based bioanalytical applications.

  5. Flow injection determination of diclofenac sodium based on its sensitizing effect on the chemiluminescent reaction of acidic potassium permanganate-formaldehyde.

    Science.gov (United States)

    Song, Jingjing; Sun, Pulv; Ji, Zhongling; Li, Jianguo

    2015-02-01

    A sensitive and simple chemiluminescent (CL) method for the determination of diclofenac sodium has been developed by combining the flow injection technique and its sensitizing effect on the weak CL reaction between formaldehyde and acidic potassium permanganate. A calibration curve is constructed for diclofenac sodium under optimized experimental parameters over the range 0.040-5.0 µg/mL and the limit of detection is 0.020 µg/mL (3σ). The inter-assay relative standard deviation for 0.040 µg/mL diclofenac sodium (n = 11) is 2.0%. This method is rapid, sensitive, simple, and shows good selectivity and reproducibility. The proposed method has been successfully applied to the determination of the studied diclofenac sodium in pharmaceutical preparations with satisfactory results. Furthermore, the possible mechanism for the CL reaction has been discussed in detail on the basis of UV and CL spectra. PMID:24802238

  6. Enzyme-linked immunosorbent assay by enhanced chemiluminescence detection for the standardization of estrogenic miroestrol in Pueraria candollei Graham ex Benth.

    Science.gov (United States)

    Yusakul, Gorawit; Udomsin, Orapin; Tanaka, Hiroyuki; Morimoto, Satoshi; Juengwatanatrakul, Thaweesak; Putalun, Waraporn

    2015-08-01

    Miroestrol (ME) is a potent phytoestrogen from the P. candollei tuberous root. It has been approved for use in clinical trials due to its beneficial effect on disorders associated with estrogen deficiency. To ensure medical efficacy and safety, high performance analytical methods for ME analysis are required to standardize products from the P. candollei root. An enhanced chemiluminescence enzyme-linked immunosorbent assay (ECL-ELISA) was developed and validated using a polyclonal antibody against ME and a chemiluminescent system of luminol-H2 O2 -horseradish peroxidase-4-(1-imidazolyl) phenol. The ECL-ELISA system exhibited linearity over a concentration range of 0.31-10.00 ng mL(-1) , for which the relative standard variation (%RSD) was less than 10% for both intra- and interplate determinations. The ECL-ELISA is reliable for the determination of ME as reflected by the high recovery percentage (101.22-103.06%). As a comparative analysis, the ME content in each sample determined by ECL-ELISA was correlated with high coefficients of determination with colorimetric ELISA (R(2)  = 0.998) and high performance liquid chromatography (HPLC) (R(2)  = 0.998) methods. The ECL-ELISA method could be applied to all of the commercial products containing P. candollei root, when the products contain between 0.706 ± 0.046 and 13.123 ± 0.794 µg g(-1) dry wt. of ME. This method is useful as a high performance analytical method for the quantity control of ME in raw materials and end products at both the research and industrial levels.

  7. Suitability of thermoluminescence, chemiluminescence, ESR and viscosity measurements as detection method for the irradiation of medicinal herbs; Eignung von Thermolumineszenz-, Chemilumineszenz-, ESR- und Viskositaetsmessungen zur Identifizierung strahlenbehandelter Arzneidrogen

    Energy Technology Data Exchange (ETDEWEB)

    Schuettler, C.; Gebhardt, G.; Stock, A.; Helle, N.; Boegl, K.W.

    1993-12-31

    Chemiluminescence, electron spin resonance, thermoluminescence and viscosity measurements have been investigated for their suitability as detection method for the irradiation of the medicinal herbs anise seeds (anisi fructus), valerian roots (valerianae radix), redberry leaves (uvae ursi folium), birch leaves (betulae folium), greek hay seeds (foenugraeci semen), cayenne pepper (capsici fructus acer), black-aldertee bark (frangulae cortex), fennel fruits (feoniculi fructus), rose hip shells (cynosbati fructus), coltsfoot (farfarae folium), acorus roots (calami rhizoma), chamomile flowers (matricariae flos), caraway (carvi fructus), lavender flowers (lavandulae flos), linseed (lini semen), lime tree flowers (tiliae flos), St. Mary`s thistle fruit (cardui mariae herba), lemon balm (melissae folium), java tea (orthosiphonis folium), peppermint (menthae piperitae folium), sage leaves (salviae folium), scouring rush (equiseti herba), senna leaves (sennae folium), plantain herbs (plantaginis lanceolata herba), thyme herbs (thymi herba), juniper berries (juniperi fructus), hawthorne herbs (crataegi folium), wheat starch (amylum tritici) and wormwood (absinthii herba). Depending on the herbs, the methods used were more or less suitable. Chemiluminescence measurements showed the smallest differences between untreated and irradiated samples whereas thermoluminescence measurements on isolated minerals from the vegetable drugs gave better results. In some herbs radiation-specific radicals could be identified by ESR spectroscopy. Viscosity measurement is suitable for some herbs as fast and inexpensive method for screening. (orig.) [Deutsch] Die Eignung von Chemilumineszenz-, ESR-, Thermolumineszenz- und Viskositaetsmessungen als Nachweismethode fuer die Behandlung von Arzneidrogen mit ionisierenden Strahlen wurde an Anis, Baldrianwurzel, Baerentraubenblaettern, Birkenblaettern, Bockshornsamen, Cayennepfeffer, Faulbaumrinde, Fenchel, Hagebuttenschalen, Huflattichblaettern

  8. An enhanced chemiluminescence resonance energy transfer system based on target recycling G-guadruplexes/hemin DNAzyme catalysis and its application in ultrasensitive detection of DNA.

    Science.gov (United States)

    Chen, Jia; Huang, Yong; Vdovenko, Marina; Sakharov, Ivan Yu; Su, Guifa; Zhao, Shulin

    2015-06-01

    An enhanced chemiluminescence resonance energy transfer (CRET) system based on target recycling G-guadruplexes/hemin DNAzyme catalysis was developed for ultrasensitive detection of DNA. CRET system consists of luminol as chemiluminescent donor, and fluorescein isothiocyanate (FITC) as acceptor. The sensitive detection was achieved by using the system consisted of G-riched DNA, blocker DNA, and the Nb.BbvCI biocatalyst. Upon addition of target DNA to the system, target DNA hybridizes with the quasi-circular DNA structure, and forms a DNA duplex. The formation of DNA duplex triggers selective enzymatic cleavage of quasi-circular DNA by Nb.BbvCI, resulting in the release of target DNA and two G-riched DNAzyme segments. Released target DNA then hybridizes with another quasi-circular DNA structure to initiate the cleavage of the quasi-circular DNA structure. Eventually, each target DNA can go through many cycles, resulting in the digestion of many quasi-circular DNA structures, generating many G-riched DNAzyme segments. G-riched DNAzyme segment products assemble with hemin to form stable hemin/G-quadruplexes that exhibit peroxidase-like activity which can catalyze the oxidation of luminol by H2O2 to produce CL signals. In the presence of FITC, CL of luminol can excite FITC molecules, and thus produced CRET between the luminol and FITC. This unique analysis strategy gives a detection limit down to 80 fM, which is at least four orders of magnitude lower than that of unamplified DNA detection methods.

  9. A sensitive inhibition chemiluminescence method for the determination of 6-mercaptopurine in tablet and biological fluid using the reaction of luminol-Ag(III) complex in alkaline medium

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Hanwen, E-mail: hanwen@hbu.edu.cn [College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002 (China); Wang, Ting; Liu, Xuyang; Chen, Peiyun [College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002 (China)

    2013-02-15

    A sensitive inhibition chemiluminescence (CL) method for the determination of 6-mercaptopurine (6-MP) is developed. The mechanism of the CL reaction between Ag(III) complex {l_brace}[Ag(HIO{sub 6}){sub 2}]{sup 5-}{r_brace} and luminol in alkaline solution was proposed, along with the inhibition mechanism of 6-MP on the CL emission. The inhibition degree of CL emission was proportional to the logarithm of 6-MP concentration. The effects of the reaction conditions on CL emission and inhibition were examined. Under the optimized conditions, the detection limit (s/n=3) was 3.7 Multiplication-Sign 10{sup -10} g ml{sup -1}. The recoveries of 6-MP were in the range of 97.7-105% with the RSD of 2.1-3.4% (n=5) for tablet samples, 103-106% with the RSDs of 1.1-2.1% for spiked serum sample, and 97.2-101% with the RSD of 2.0-4.5% for spiked urine sample. The accuracy of this method for the tablet analysis was examined by comparing with the pharmacopoeia method. The proposed method was used for the determination of 6-MP at clinically relevant concentrations in real urine and serum samples with satisfactory results. - Highlights: Black-Right-Pointing-Pointer A sensitive inhibition chemiluminescence (CL) method for the determination of 6-MP is developed. Black-Right-Pointing-Pointer The inhibition mechanism of 6-MP on the CL emission was proposed. Black-Right-Pointing-Pointer The detection limit was 3.7 Multiplication-Sign 10{sup -10} g ml{sup -1}. Black-Right-Pointing-Pointer The accuracy was examined by comparing with the pharmacopoeia method.

  10. 3-(10'-Phenothiazinyl)propionic acid is a potent primary enhancer of peroxidase-induced chemiluminescence and its application in sensitive ELISA of methylglyoxal-modified low density lipoprotein.

    Science.gov (United States)

    Sakharov, Ivan Yu; Demiyanova, Alexandra S; Gribas, Anastasia V; Uskova, Natalia A; Efremov, Evgeny E; Vdovenko, Marina M

    2013-10-15

    Using a full factorial design the optimization of experimental conditions of enhanced chemiluminescence reaction (ECR) catalyzed by horseradish peroxidase (HRP) in the presence of 3-(10'-phenothiazinyl)propionic acid (PPA) as a primary enhancer was performed. The effect of concentrations of PPA, hydrogen peroxide, MORPH, luminol, and Tris on a ratio of peroxidase-catalyzed CL to background was studied. The detection limit value of HRP in ECR with PPA was 0.09 pM. Using PPA the ultra-sensitive chemiluminescent ELISA for determination of methylglyoxal-modified low density lipoprotein was developed. The detection limit value for the developed method was 0.5 ng mL(-1). The obtained results open up very promising perspectives for using PPA to improve the sensitivity of enzyme immunoassay kits.

  11. The determination of psilocin and psilocybin in hallucinogenic mushrooms by HPLC utilizing a dual reagent acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection system.

    Science.gov (United States)

    Anastos, Nicole; Lewis, Simon W; Barnett, Neil W; Sims, D Noel

    2006-01-01

    This paper describes a procedure for the determination of psilocin and psilocybin in mushroom extracts using high-performance liquid chromatography with postcolumn chemiluminescence detection. A number of extraction methods for psilocin and psilocybin in hallucinogenic mushrooms were investigated, with a simple methanolic extraction being found to be most effective. Psilocin and psilocybin were extracted from a variety of hallucinogenic mushrooms using methanol. The analytes were separated on a C12 column using a (95:5% v/v) methanol:10 mM ammonium formate, pH 3.5 mobile phase with a run time of 5 min. Detection was realized through a dual reagent chemiluminescence detection system of acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II). The chemiluminescence detection system gave improved detectability when compared with UV absorption at 269 nm, with detection limits of 1.2 x 10(-8) and 3.5 x 10(-9) mol/L being obtained for psilocin and psilocybin, respectively. The procedure was applied to the determination of psilocin and psilocybin in three Australian species of hallucinogenic mushroom.

  12. Ultrasensitive detection of cancer cells and glycan expression profiling based on a multivalent recognition and alkaline phosphatase-responsive electrogenerated chemiluminescence biosensor

    Science.gov (United States)

    Chen, Xiaojiao; He, Yao; Zhang, Youyu; Liu, Meiling; Liu, Yang; Li, Jinghong

    2014-09-01

    A multivalent recognition and alkaline phosphatase (ALP)-responsive electrogenerated chemiluminescence (ECL) biosensor for cancer cell detection and in situ evaluation of cell surface glycan expression was developed on a poly(amidoamine) (PAMAM) dendrimer-conjugated, chemically reduced graphene oxide (rGO) electrode interface. In this strategy, the multivalency and high affinity of the cell-targeted aptamers on rGO provided a highly efficient cell recognition platform on the electrode. The ALP and concanavalin A (Con A) coated gold nanoparticles (Au NPs) nanoprobes allowed the ALP enzyme-catalyzed production of phenols that inhibited the ECL reaction of Ru(bpy)32+ on the rGO electrode interface, affording fast and highly sensitive ECL cytosensing and cell surface glycan evaluation. Combining the multivalent aptamer interface and ALP nanoprobes, the ECL cytosensor showed a detection limit of 38 CCRF-CEM cells per mL in human serum samples, broad dynamic range and excellent selectivity. In addition, the proposed biosensor provided a valuable insight into dynamic profiling of the expression of different glycans on cell surfaces, based on the carbohydrates recognized by lectins applied to the nanoprobes. This biosensor exhibits great promise in clinical diagnosis and drug screening.A multivalent recognition and alkaline phosphatase (ALP)-responsive electrogenerated chemiluminescence (ECL) biosensor for cancer cell detection and in situ evaluation of cell surface glycan expression was developed on a poly(amidoamine) (PAMAM) dendrimer-conjugated, chemically reduced graphene oxide (rGO) electrode interface. In this strategy, the multivalency and high affinity of the cell-targeted aptamers on rGO provided a highly efficient cell recognition platform on the electrode. The ALP and concanavalin A (Con A) coated gold nanoparticles (Au NPs) nanoprobes allowed the ALP enzyme-catalyzed production of phenols that inhibited the ECL reaction of Ru(bpy)32+ on the rGO electrode

  13. 氯霉素化学发光酶联免疫检测方法的建立%Establishment of Chemiluminescent Enzyme Immunoassay for Chloramphenicol

    Institute of Scientific and Technical Information of China (English)

    何方洋; 冯月君; 吴鹏; 冯静; 岳新荣; 韩京朋

    2012-01-01

    A convenient, rapid and accurate one step chloramphenicol (CAP) residue chemiluminescent enzyme immunoassay(CLEIA) detection method was established based on conventional ELISA, by using HRP labeled monoclonal antibodies obtained from immunizing mice that were injected with CAP - BSA coupling protein, and optimizing test parameters, such as antibody concentration, reaction time. The results showed that the best detection range of this method was 20 -1620 pg/mL, the ICs0 was 59. 911 pg/mL, the detection limit was 9.3 pg/g. It can check out the international provisions standard limited value of the CAP residue. Compared with conventional ELISA test method, the CLEIA method can not only increase the detection limit and sensitivity, but also shorten the reaction time by 60 minutes. It indicated that using the one step chemiluminescent enzyme immunoassay kit to detect chloramphenicol residue can decrease time consuming, and was more appropriate for fast detection requirements.%在常规酶联免疫法(ELISA)的基础上,用辣根过氧化物酶标记氯霉素与牛血清白蛋白偶联后免疫小鼠制得的单克隆抗体,并优化抗体工作浓度、反应时间等试验参数,建立了一种简便、快速、准确的氯霉素(CAP)残留一步式化学发光酶联免疫检测方法。结果表明:该方法最佳检测范围为20~1620pg/mL,IC50值达59.911pg/mL,检测限为9.3Pg/g,可检出国际规定的CAP残留标准限量值以下的残留量。与常规ELISA检测方法相比,不仅提高了检测限和灵敏度,而且反应时间缩短了60min。采用一步式化学发光酶联免疫检测氯霉素残留,可以大幅度缩减检测时间,更符合快速检测的要求。

  14. Flow injection chemiluminescence determination of cephalosporins in pharmaceutical preparations using tris (2,2'-bipyridyl) ruthenium (II)-potassium permanganate system

    International Nuclear Information System (INIS)

    A flow injection (FI) method using the tris(2,2'-bipyridyl) ruthenium(II) [Ru(bpy)32+]-potassium permanganate chemiluminescence(CL) was developed for the rapid and sensitive determination of cephalosporins such as cefoxitin, cefazolin, cephalexin, cefadroxil, cefaclor and cefoperazone. The method is based on the CL reaction of cephalosporins and Ru(bpy)32+ with potassium permanganate in the presence of perchloric acid, catalyzed by Mn(II). Under the optimum conditions, the calibration curves are linear over the range 0.10-12.0 μg ml-1 for cefoxitin, 0.10-10.0 μg ml-1 for cefoperazone and 0.10-15.0 μg ml-1 for cefazolin, cephalexin, cefadroxil, and cefaclor, respectively. The limits of detection (3σ) are 0.03 μg ml-1 for cefoxitin and cefadroxil, 0.06 μg ml-1 for cefoperazone and 0.08 μg ml-1 for cefazolin, cephalexin and cefaclor, respectively. The method was successfully applied to the determination of cefoxitin, cefazolin, cephalexin and cefadroxil in pharmaceutical formulations with a sample throughput of 90 h-1. There were no interference effects from some common excipients used in pharmaceutical preparations

  15. Preparation of polyclonal antibodies for nateglinide (NTG) and development of a sensitive chemiluminescent immunoassay to detect NTG in tablets and serum.

    Science.gov (United States)

    Zheng, Lei; Wang, Jing; Zhang, Jie; Song, Zhaorui; Dong, Yaqing; Wang, Yufen; Tong, Zhongsheng; Deng, Chuan; Yin, Yongmei; Meng, Meng; Xi, Rimo

    2016-01-01

    In this study, we prepared polyclonal antibodies against anti-diabetic drug nateglinide (NTG), and established a sensitive chemiluminescent immunoassay (CLIA) to detect NTG in tablets and serum. Two kinds of immunogens were synthesized using ethylcarbodiimide (EDC)/hydroxysuccinimide (NHS) and carbonyldiimidazole (CDI)/4-dimethylaminopyridine (DMAP) as coupling reagents respectively. When activated by EDC/NHS, more molecules of NTG coupled with carrier protein in immunogens. A horseradish peroxidase (HRP)-luminol-H2O2 system with p-iodophenol enhancement was applied in the CLIA analysis. The antibodies in EDC/NHS group showed higher titer, sensitivity and wider detection linear range than those in CDI/DMAP group, and were chosen for next studies. The developed CLIA assay exhibited good selectivity towards NTG among structually similar analogs. The method could detect as low as 0.35 ng mL(-1) NTG in buffer, 2.1 ng mL(-1) NTG in serum and 0.84 ng mL(-1) NTG in tablets. The CLIA method provided consistent results with HPLC method (r=0.9986) in determination of NTG from 5.0 to 400 µg mL(-1). The CLIA method could detect 78 samples in one assay, and the samples need only dilution in pretreatment. As a summary, this research offers a sensitive assay for high-throughout screening of NTG in formulation control and pharmacokinetic studies. PMID:26695294

  16. Screening of cannabinoids in industrial-grade hemp using two-dimensional liquid chromatography coupled with acidic potassium permanganate chemiluminescence detection.

    Science.gov (United States)

    Pandohee, Jessica; Holland, Brendan J; Li, Bingshan; Tsuzuki, Takuya; Stevenson, Paul G; Barnett, Neil W; Pearson, James R; Jones, Oliver A H; Conlan, Xavier A

    2015-06-01

    Widely known for its recreational use, the cannabis plant also has the potential to act as an antibacterial agent in the medicinal field. The analysis of cannabis plants/products in both pharmacological and forensic studies often requires the separation of compounds of interest and/or accurate identification of the whole cannabinoid profile. In order to provide a complete separation and detection of cannabinoids, a new two-dimensional liquid chromatography method has been developed using acidic potassium permanganate chemiluminescence detection, which has been shown to be selective for cannabinoids. This was carried out using a Luna 100 Å CN column and a Poroshell 120 EC-C18 column in the first and second dimensions, respectively. The method has utilized a large amount of the available separation space with a spreading angle of 48.4° and a correlation of 0.66 allowing the determination of more than 120 constituents and mass spectral identification of ten cannabinoids in a single analytical run. The method has the potential to improve research involved in the characterization of sensitive, complex matrices.

  17. Electrogenerated chemiluminescence reactions between the [Ru(bpy)3](2+) complex and PAMAM GX.0 dendrimers in an aqueous medium.

    Science.gov (United States)

    Jimenez-Ruiz, A; Grueso, E; Perez-Tejeda, P

    2015-10-01

    Electrogenerated chemiluminescence, ECL, reactions between tris(2,2'-bipyridine)ruthenium(II), [Ru(bpy)3](2+), and PAMAM GX.0 (X=1 and 2) dendrimers in an aqueous medium were carried out at pH10 (fully deprotonated dendrimer surface). ECL was detected in the presence of GX.0 dendrimers without addition of any known coreactant. Atomic force microscopy, AFM, measurements for GX.0 dendrimers in the presence of the [Ru(bpy)3](2+) complex were also done. AFM images showed the existence of aggregates (pillars) of globular shape, as well as interdendrimer networks forming fibers in the x-y direction for dendrimer aqueous solutions. ECL and AFM results in cooperation suggest that the coreactant effect of the end amine groups is improved by both the dendritic branched shells and the globular z-type aggregates. The ECL efficiency trends as a function of [GX.0] (whole range) can be interpreted taking into account the coreactant effect modulated by the presence of the z and x-y type aggregates. Importantly, ECL efficiency values can be taken as a measure of the change induced on the dendrimer aggregation in aqueous solutions when their concentrations rise. Redox potentials of the [Ru(bpy)3](3+/2+) couple in the presence of the G1.0 and G2.0 dendrimers were also determined. PMID:26190671

  18. Chemiluminescence immunoassay for the rapid and sensitive detection of antibody against porcine parvovirus by using horseradish peroxidase/detection antibody-coated gold nanoparticles as nanoprobes.

    Science.gov (United States)

    Zhou, Yuan; Zhou, Tao; Zhou, Rui; Hu, Yonggang

    2014-06-01

    A rapid, simple, facile, sensitive and enzyme-amplified chemiluminescence immunoassay (CLIA) method to detect antibodies against porcine parvovirus has been developed. Horseradish peroxidase (HRP) and the detection antibody were simultaneously co-immobilized on the surface of gold nanoparticles using the electrostatic method to form gold nanoparticle-based nanoprobes. This nanoprobe was employed in a sandwich-type CLIA, which enables CL signal readout from enzymatic catalysis and results in signal amplification. The presence of porcine parvovirus infection was determined in porcine parvovirus antibodies by measuring the CL intensity caused by the reaction of HRP-luminol with H2 O2 . Under optimal conditions, the obtained calibration plot for the standard positive serum was approximately linear within the dilution range of 1:80 to 1:5120. The limit of detection for the assay was 1:10,240 (S/N = 3), which is much lower than that typically achieved with an enzyme-linked immunosorbent assay (1:160; S/N = 3). A series of repeatability measurements using 1:320-fold diluted standard positive serum gave reproducible results with a relative standard deviation of 4.9% (n = 11). The ability of the immunosensor to analyze clinical samples was tested on porcine sera. The immunosensor had an efficiency of 90%, a sensitivity of 93.3%, and a specificity of 87.5% relative to the enzyme-linked immunosorbent assay results.

  19. Considering the chemical energy requirements of the tri-n-propylamine co-reactant pathways for the judicious design of new electrogenerated chemiluminescence detection systems.

    Science.gov (United States)

    Kerr, Emily; Doeven, Egan H; Wilson, David J D; Hogan, Conor F; Francis, Paul S

    2016-01-01

    The introduction of a 'co-reactant' was a critical step in the evolution of electrogenerated chemiluminescence (ECL) from a laboratory curiosity to a widely utilised detection system. In conjunction with a suitable electrochemiluminophore, the co-reactant enables generation of both the oxidised and reduced precursors to the emitting species at a single electrode potential, under the aqueous conditions required for most analytical applications. The most commonly used co-reactant is tri-n-propylamine (TPrA), which was developed for the classic tris(2,2'-bipyridine)ruthenium(II) ECL reagent. New electrochemiluminophores such as cyclometalated iridium(III) complexes are also evaluated with this co-reactant. However, attaining the excited states in these systems can require much greater energy than that of tris(2,2'-bipyridine)ruthenium(II), which has implications for the co-reactant reaction pathways. In this tutorial review, we describe a simple graphical approach to characterise the energetically feasible ECL pathways with TPrA, as a useful tool for the development of new ECL detection systems. PMID:26525583

  20. Chemiluminescent labels released from long spacer arm-functionalized magnetic particles: a novel strategy for ultrasensitive and highly selective detection of pathogen infections.

    Science.gov (United States)

    Yang, Haowen; Liang, Wenbiao; He, Nongyue; Deng, Yan; Li, Zhiyang

    2015-01-14

    Previously, the unique advantages provided by chemiluminescence (CL) and magnetic particles (MPs) have resulted in the development of many useful nucleic acid detection methods. CL is highly sensitive, but when applied to MPs, its intensity is limited by the inner filter-like effect arising from excess dark MPs. Herein, we describe a modified strategy whereby CL labels are released from MPs to eliminate this negative effect. This approach relies on (1) the magnetic capture of target molecules on long spacer arm-functionalized magnetic particles (LSA-MPs), (2) the conjugation of streptavidin-alkaline phosphatase (SA-AP) to biotinylated amplicons of target pathogens, (3) the release of CL labels (specifically, AP tags), and (4) the detection of the released labels. CL labels were released from LSA-MPs through LSA ultrasonication or DNA enzymolysis, which proved to be the superior method. In contrast to conventional MPs, LSA-MPs exhibited significantly improved CL detection, because of the introduction of LSA, which was made of water-soluble carboxymethylated β-1,3-glucan. Detection of hepatitis B virus with this technique revealed a low detection limit of 50 fM, high selectivity, and excellent reproducibility. Thus, this approach may hold great potential for early stage clinical diagnosis of infectious diseases.

  1. An ultra-facile and label-free immunoassay strategy for detection of copper (II) utilizing chemiluminescence self-enhancement of Cu (II)-ethylenediaminetetraacetate chelate.

    Science.gov (United States)

    Ouyang, Hui; Shu, Qi; Wang, Wenwen; Wang, Zhenxing; Yang, Shijia; Wang, Lin; Fu, Zhifeng

    2016-11-15

    The establishment of facile, rapid, sensitive and cost-effective protocols for the detection of heavy metals is of great significance for human health and environmental monitoring. Hereby, an ultra-facile and label-free immunoassay strategy was designed for detecting heavy metal ion by using Cu (II) as the model analyte. Cu (II) reacted previously with ethylenediaminetetraacetate (EDTA) was captured by immobilized monoclonal antibody for Cu (II)-EDTA chelate. Then Cu (II) was detected based on the self-enhancing effect of Cu (II)-EDTA chelate to luminol-H2O2 chemiluminescence reaction. The CL intensity is linear relative with Cu (II) concentration in a very wide range of 1.0-1000ng/mL, with a detection limit of 0.33ng/mL (S/N=3). Since the specificity of this proposed strategy relied on both the specificity of monoclonal antibody and the specificity of luminol-H2O2 system, it could avoid interference from most common ions. The proposed method was used successfully to detect Cu (II) in traditional Chinese medicine and environmental water samples with acceptable recovery values of 82-113%. This proof-of-principle work demonstrated the feasibility of the label-free immunoassay for heavy metal ions, and opened a new avenue for rapid screening and field assay for drug safety, environmental monitoring and clinical diagnosis.

  2. Increased level of phosphorylated akt measured by chemiluminescence-linked immunosorbent assay is a predictor of poor prognosis in primary breast cancer overexpressing ErbB-2

    International Nuclear Information System (INIS)

    Akt1, Akt2 and Akt3 kinases are downstream components of phosphoinositol 3-kinase derived signals from receptor tyrosine kinases, which influence cell growth, proliferation and survival. Akt2 overexpression and amplification have been described in breast, ovarian and pancreatic cancers. The present study was designed to investigate the prognostic significance of activated Akt in primary breast cancer and its association with other tumour biomarkers. Using a two-site chemiluminescence-linked immunosorbent assay, we measured the quantitative expression levels of total phosphorylated (P-S473) Akt (Akt1/Akt2/Akt3) on cytosol fractions obtained from fresh frozen tissue samples of 156 primary breast cancer patients. Akt phosphorylation was not associated with nodal status or ErbB-2 protein expression levels. High levels of phosphorylated Akt correlated (P < 0.01) with poor prognosis, and the significance of this correlation increased (P < 0.001) in the subset of patients with ErbB-2 overexpressing tumours. In addition, phosphorylated Akt was found to be associated with mRNA expression levels of several proliferation markers (e.g. thymidylate synthase), measured using quantitative real-time RT-PCR. Our findings demonstrate that, in breast cancer patients, Akt activation is associated with tumour proliferation and poor prognosis, particularly in the subset of patients with ErbB2-overexpressing tumours

  3. Comparison of two methods for selegiline determination: A flow-injection chemiluminescence method using cadmium sulfide quantum dots and corona discharge ion mobility spectrometry

    Science.gov (United States)

    Khataee, Alireza; Lotfi, Roya; Hasanzadeh, Aliyeh; Iranifam, Mortaza; Zarei, Mahmoud; Joo, Sang Woo

    2016-01-01

    Two analytical approaches including chemiluminescence (CL) and corona discharge ionization ion mobility spectrometry (CD-IMS) were developed for sensitive determination of selegiline (SG). We found that the CL intensity of the KMnO4-Na2S2O3 CL system was significantly enhanced in the presence of L-cysteine capped CdS quantum dots (QDs). A possible CL mechanism for this CL reaction is proposed. In the presence of SG, the enhanced CL system was inhibited. Based on this inhibition, a simple and sensitive flow-injection CL method was proposed for the determination of SG. Under optimum experimental conditions, the decreased CL intensity was proportional to SG concentration in the range of 0.01 to 30.0 mg L- 1. The detection limit (3σ) was 0.004 mg L- 1. Also, SG was determined using CD-IMS, and under optimum conditions of CD-IMS, calibration curves were linear in the range of 0.15 to 42.0 mg L- 1, with a detection limit (3σ) of 0.03 mg L- 1. The precision of the two methods was calculated by analyzing samples containing 5.0 mg L- 1 of SG (n = 11). The relative standard deviations (RSDs%) of the flow-injection CL and CD-IMS methods are 2.17% and 3.83%, respectively. The proposed CL system exhibits a higher sensitivity and precision than the CD-IMS method for the determination of SG.

  4. 化学发光法测定蜂蜜中土霉素残留含量%Determination of Oxytetracycline Residue in Honey by Chemiluminescence Analysis

    Institute of Scientific and Technical Information of China (English)

    竹磊; 章建军; 田春华; 朱海华; 李向力

    2011-01-01

    The oxytetracycline residue in honey was determined by using chemiluminescence analysis. The results showed that there was good linear relationship between the relative light unit and oxytetracycline residue at the relatively low mass concentration of 0 -10 x 10 -7 g/mL and the relatively high mass concentration of (20 ~ 100) x 10 -7 g/mL, the relative standard deviation was 1.97% , and the average recovery rate was 97.5% and 98.2% respectively. This method is rapid, simple, accurate and reliable.%采用化学发光法对蜂蜜中土霉素残留含量进行测定,结果表明:相对发光值△I与土霉素在相对低质量浓度0~10×10 -7g/mL和相对高质量浓度(20 ~100)×10-7g/mL范围内呈良好线性关系,相对标准偏差为1.97%,平均回收率为97.5%和98.2%.该法操作快速、简便,结果准确、可靠.

  5. Simultaneous automatic determination of catecholamines and their 3-O-methyl metabolites in rat plasma by high-performance liquid chromatography using peroxyoxalate chemiluminescence reaction.

    Science.gov (United States)

    Tsunoda, M; Takezawa, K; Santa, T; Imai, K

    1999-05-01

    A highly specific and sensitive automated high-performance liquid chromatographic method for the simultaneous determination of catecholamines (CAs; norepinephrine, epinephrine, and dopamine) and their 3-O-methyl metabolites (normetanephrine, metanephrine, and 3-methoxytyramine) is described. Automated precolumn ion-exchange extraction of diluted plasma is coupled with HPLC separation of CAs and their 3-O-methyl metabolites on an ODS column, postcolumn coulometric oxidation, fluorescence derivatization with ethylenediamine, and finally peroxyoxalate chemiluminescence reaction detection. The detection limits were about 3 fmol for norepinephrine, epinephrine, and dopamine, 5 fmol for normetanephrine, and 10 fmol for metanephrine and 3-methoxytyramine (signal-to-noise ratio of 3). Fifty microliters of rat plasma was used and 4-methoxytyramine was employed as an internal standard. The relative standard deviations for the method (n = 5) were 2.5-7.6% for the intraday assay and 6.3-9.1% for the interday assay. The method was applicable to the determination of normetanephrine and metanephrine in 50 microl of rat plasma. PMID:10222014

  6. Investigation of iron(III) reduction and trace metal interferences in the determination of dissolved iron in seawater using flow injection with luminol chemiluminescence detection

    Energy Technology Data Exchange (ETDEWEB)

    Ussher, Simon J. [School of Earth, Ocean and Environmental Sciences (SEOES), University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Milne, Angela [School of Earth, Ocean and Environmental Sciences (SEOES), University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Department of Oceanography, Florida State University, Tallahassee, FL 32306-4320 (United States); Landing, William M. [Department of Oceanography, Florida State University, Tallahassee, FL 32306-4320 (United States); Attiq-ur-Rehman, Kakar [Department of Chemistry, University of Balochistan, Quetta (Pakistan); Seguret, Marie J.M.; Holland, Toby [School of Earth, Ocean and Environmental Sciences (SEOES), University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom); Achterberg, Eric P. [National Oceanography Centre, University of Southampton, European Way, Southampton SO14 3ZH (United Kingdom); Nabi, Abdul [Department of Chemistry, University of Balochistan, Quetta (Pakistan); Worsfold, Paul J., E-mail: pworsfold@plymouth.ac.uk [School of Earth, Ocean and Environmental Sciences (SEOES), University of Plymouth, Drake Circus, Plymouth PL4 8AA (United Kingdom)

    2009-10-12

    A detailed investigation into the performance of two flow injection-chemiluminescence (FI-CL) manifolds (with and without a preconcentration column) for the determination of sub-nanomolar dissolved iron (Fe(II) + Fe(III)), following the reduction of Fe(III) by sulphite, in seawater is described. Kinetic experiments were conducted to examine the efficiency of reduction of inorganic Fe(III) with sulphite under different conditions and a rigorous study of the potential interference caused by other transition metals present in seawater was conducted. Using 100 {mu}M concentrations of sulphite a reduction time of 4 h was sufficient to quantitatively reduce Fe(III) in seawater. Under optimal conditions, cobalt(II) and vanadium(IV)/(III) were the major positive interferences and strategies for their removal are reported. Specifically, cobalt(II) was masked by the addition of dimethylglyoxime to the luminol solution and vanadium(IV) was removed by passing the sample through an 8-hydroxyquinoline column in a low pH carrier stream. Manganese(II) also interfered by suppression of the CL response but this was not significant at typical open ocean concentrations.

  7. Simultaneous identification and quantification of new psychoactive substances in blood by GC-APCI-QTOFMS coupled to nitrogen chemiluminescence detection without authentic reference standards.

    Science.gov (United States)

    Ojanperä, Ilkka; Mesihää, Samuel; Rasanen, Ilpo; Pelander, Anna; Ketola, Raimo A

    2016-05-01

    A novel platform is introduced for simultaneous identification and quantification of new psychoactive substances (NPS) in blood matrix, without the necessity of using authentic reference standards. The instrumentation consisted of gas chromatography (GC) coupled to nitrogen chemiluminescence detection (NCD) and atmospheric pressure chemical ionization quadrupole time-of-flight mass spectrometry (APCI-QTOFMS). In this concept, the GC flow is divided in appropriate proportions between NCD for single-calibrant quantification, utilizing the detector's equimolar response to nitrogen, and QTOFMS for accurate mass-based identification. The principle was proven by analyzing five NPS, bupropion, desoxypipradrol (2-DPMP), mephedrone, methylone, and naphyrone, in sheep blood. The samples were spiked with the analytes post-extraction to avoid recovery considerations at this point. All the NPS studies produced a protonated molecule in APCI resulting in predictable fragmentation with high mass accuracy. The N-equimolarity of quantification by NCD was investigated by using external calibration with the secondary standard caffeine at five concentration levels between 0.17 and 1.7 mg/L in blood matrix as five replicates. The equimolarity was on average 98.7%, and the range of individual equimolarity determinations was 76.7-130.1%. The current analysis platform affords a promising approach to instant simultaneous qualitative and quantitative analysis of drugs in the absence of authentic reference standards, not only in forensic and clinical toxicology but also in other bioanalytical applications.

  8. A simple and rapid flow-injection chemiluminescence method for the determination of noscapine with Ru(phen)3(2+)-Ce(IV) system.

    Science.gov (United States)

    Rezaei, Behzad; Mokhtari, Ali; Khayamian, Taghi

    2007-08-01

    A new flow injection chemiluminescence (CL) system was used for the determination of noscapine. This technique is based on the reduction effect of noscapine on the Ru(phen)3(3+), which is produced by reaction between Ru(phen)3(2+) and acidic Ce(IV) solutions, and this rapid reduction produces strong CL. Calibration plots were linear over the range of 3.0 x 10(-7) - 2.0 x 10(-6) mol L(-1) and 2.0 x 10(-6) - 2.0 x 10(-4) mol L(-1). The CL intensity was so high, that it is able to produce a detection limit of 6.6 x 10(-8) M noscapine (3sigma). The relative standard deviation of 2.0 x 10(-6) M noscapine was 1.0% (n=10). The proposed method was successfully applied for the flow injection determination of noscapine in cough and Tonin syrup samples. The results of real sample analyses show good recovery percentages (97.3-102.4%). The minimum sampling rate was 100 samples per hour. PMID:17899875

  9. Adaptation of a load-inject valve for a flow injection chemiluminescence system enabling dual-reagent injection enhances understanding of environmental Fenton chemistry

    International Nuclear Information System (INIS)

    Graphical abstract: -- Highlights: •Measurement of multiple components of Fenton chemistry; Fe(II) and H2O2. •Rapid, quasi-simultaneous analysis enables calculation of environmental kinetics. •Low, nano to pico-molar detection limits with dual analyte analysis. •Able to measure complex matrix samples – organically enriched seawater. •Low cost system with appreciable sensitivity compared to single analyte analysis. -- Abstract: Environmental Fenton chemistry has been poorly constrained within the marine environment at a multi-component level. A simple, unique, reconfiguration of a flow-injection analytical system combined with luminol chemiluminescence allows quasi-simultaneously the measurement, using a single load-inject valve and a single photon multiplier tube, of reduced iron, Fe(II), and hydrogen peroxide. The system enables rapid, every 22 s, measurements with good accuracy at environmentally relevant concentrations, less than 5% relative standard deviations on both a 5 nM Fe(II) standard and a 60 nM hydrogen peroxide standard. Limits of detection were as low as 40 pM Fe(II) and 100 pM hydrogen peroxide. The system showed excellent capability by measuring from within an organic rich seawater the photochemically induced production of Fe(II) and hydrogen peroxide and their subsequent cycling and Fenton like interactions

  10. Flow injection chemiluminescence determination of cephalosporins in pharmaceutical preparations using tris (2,2'-bipyridyl) ruthenium (II)-potassium permanganate system

    Energy Technology Data Exchange (ETDEWEB)

    Thongpoon, Chalermporn [Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200 (Thailand); Liawruangrath, Boonsom [Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200 (Thailand); Liawruangrath, Saisunee [Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200 (Thailand); Wheatley, R. Alan [Department of Chemistry, University of Hull, Hull HU6 7RX (United Kingdom); Townshend, Alan [Department of Chemistry, University of Hull, Hull HU6 7RX (United Kingdom)]. E-mail: A.townshend@hull.ac.uk

    2005-11-30

    A flow injection (FI) method using the tris(2,2'-bipyridyl) ruthenium(II) [Ru(bpy){sub 3} {sup 2+}]-potassium permanganate chemiluminescence(CL) was developed for the rapid and sensitive determination of cephalosporins such as cefoxitin, cefazolin, cephalexin, cefadroxil, cefaclor and cefoperazone. The method is based on the CL reaction of cephalosporins and Ru(bpy){sub 3} {sup 2+} with potassium permanganate in the presence of perchloric acid, catalyzed by Mn(II). Under the optimum conditions, the calibration curves are linear over the range 0.10-12.0 {mu}g ml{sup -1} for cefoxitin, 0.10-10.0 {mu}g ml{sup -1} for cefoperazone and 0.10-15.0 {mu}g ml{sup -1} for cefazolin, cephalexin, cefadroxil, and cefaclor, respectively. The limits of detection (3{sigma}) are 0.03 {mu}g ml{sup -1} for cefoxitin and cefadroxil, 0.06 {mu}g ml{sup -1} for cefoperazone and 0.08 {mu}g ml{sup -1} for cefazolin, cephalexin and cefaclor, respectively. The method was successfully applied to the determination of cefoxitin, cefazolin, cephalexin and cefadroxil in pharmaceutical formulations with a sample throughput of 90 h{sup -1}. There were no interference effects from some common excipients used in pharmaceutical preparations.

  11. Sensitive determination method for mercury ion, methyl-, ethyl-, and phenyl-mercury in water and biological samples using high-performance liquid chromatography with chemiluminescence detection.

    Science.gov (United States)

    Kodamatani, Hitoshi; Matsuyama, Akito; Saito, Keiitsu; Kono, Yuriko; Kanzaki, Ryo; Tomiyasu, Takashi

    2012-01-01

    A sensitive determination method for mercury speciation analysis was developed. Four mercury species, mercury ion, methylmercury, ethylmercury, and phenylmercury, were complexed with emetine-dithiocarbamate (emetine-CS(2)), and then injected onto a HPLC instrument coupled with a tris(2,2'-bipyridine)ruthenium(III) chemiluminescence detection system. The emetine-CS(2) complexing agent was effectively used to measure the concentration in addition to serving as a separation and detection reagent. The calibration curves for these mercury complexes were linear in the range of 0.050 - 10 μg L(-1) (as Hg). The limit of detection for (emetine-CS(2))(2)Hg, emetine-CS(2)-methylmercury, emetine-CS(2)-ethylmercury, and emetine-CS(2)-phenylmercury were 30, 17, 21, and 22 ng L(-1), respectively. The sensitivity of this method enables the determination of mercury species in water samples at sub-ppb levels. Furthermore, the method was applied to biological samples in combination with acid leaching and liquid-liquid extraction using emetine-CS(2) as an extraction reagent. The determination results were in good agreement with the values of the certified reference materials.

  12. Determination of medroxyprogesterone acetate in serum by HPLC with peroxyoxalate chemiluminescence detection using a fluorogenic reagent, 4-(N,N-dimethylaminosulphonyl)-7-hydrazino-2,1,3-benzoxadiazole.

    Science.gov (United States)

    Uzu, S; Imai, K; Nakashima, K; Akiyama, S

    1992-01-01

    The high-performance liquid chromatographic determination of medroxyprogesterone acetate (MPA) with peroxyoxalate chemiluminescence (PO-CL) detection is described. The spiked serum containing MPA was extracted on Bond-Elut C18 columns and derivatized with 4-(N,N-dimethylaminosulphonyl)-7-hydrazino-2,1,3-benzoxadiazole (DBD-H). The hydrazone of MPA with DBD-H was confirmed to be a mono-DBD-derivative. The reaction mixture was separated by direct injection onto a C18 analytical column, and quantified by PO-CL detection. The linear range of the standard curve, in serum, was 15.6-96.6 ng ml-1 with a detection limit of 9 ng ml-1 using only 100 microliters of serum, while the detection limit of standard MPA derivatized with DBD-H was 8.7 fmol per injection. The relative standard deviation of the method was 7.4% at 19.3 ng and 1.7% at 77.3 ng ml-1. PMID:1298405

  13. Screening of cannabinoids in industrial-grade hemp using two-dimensional liquid chromatography coupled with acidic potassium permanganate chemiluminescence detection.

    Science.gov (United States)

    Pandohee, Jessica; Holland, Brendan J; Li, Bingshan; Tsuzuki, Takuya; Stevenson, Paul G; Barnett, Neil W; Pearson, James R; Jones, Oliver A H; Conlan, Xavier A

    2015-06-01

    Widely known for its recreational use, the cannabis plant also has the potential to act as an antibacterial agent in the medicinal field. The analysis of cannabis plants/products in both pharmacological and forensic studies often requires the separation of compounds of interest and/or accurate identification of the whole cannabinoid profile. In order to provide a complete separation and detection of cannabinoids, a new two-dimensional liquid chromatography method has been developed using acidic potassium permanganate chemiluminescence detection, which has been shown to be selective for cannabinoids. This was carried out using a Luna 100 Å CN column and a Poroshell 120 EC-C18 column in the first and second dimensions, respectively. The method has utilized a large amount of the available separation space with a spreading angle of 48.4° and a correlation of 0.66 allowing the determination of more than 120 constituents and mass spectral identification of ten cannabinoids in a single analytical run. The method has the potential to improve research involved in the characterization of sensitive, complex matrices. PMID:25845561

  14. 3D origami-based multifunction-integrated immunodevice: low-cost and multiplexed sandwich chemiluminescence immunoassay on microfluidic paper-based analytical device.

    Science.gov (United States)

    Ge, Lei; Wang, Shoumei; Song, Xianrang; Ge, Shenguang; Yu, Jinghua

    2012-09-01

    A novel 3D microfluidic paper-based immunodevice, integrated with blood plasma separation from whole blood samples, automation of rinse steps, and multiplexed CL detections, was developed for the first time based on the principle of origami (denoted as origami-based device). This 3D origami-based device, comprised of one test pad surrounded by four folding tabs, could be patterned and fabricated by wax-printing on paper in bulk. In this work, a sandwich-type chemiluminescence (CL) immunoassay was introduced into this 3D origami-based immunodevice, which could separate the operational procedures into several steps including (i) folding pads above/below and (ii) addition of reagent/buffer under a specific sequence. The CL behavior, blood plasma separation, washing protocol, and incubation time were investigated in this work. The developed 3D origami-based CL immunodevice, combined with a typical luminuol-H(2)O(2) CL system and catalyzed by Ag nanoparticles, showed excellent analytical performance for the simultaneous detection of four tumor markers. The whole blood samples were assayed and the results obtained were in agreement with the reference values from the parallel single-analyte test. This paper-based microfluidic origami CL detection system provides a new strategy for a low-cost, sensitive, simultaneous multiplex immunoassay and point-of-care diagnostics. PMID:22763468

  15. Validation of a Commercial Chemiluminescence Immunoassay for the Simultaneous Measurement of Three Different Amyloid-β Peptides in Human Cerebrospinal Fluid and Application to a Clinical Cohort.

    Science.gov (United States)

    Klafki, Hans-W; Hafermann, Henning; Bauer, Chris; Haussmann, Ute; Kraus, Inga; Schuchhardt, Johannes; Muck, Stephan; Scherbaum, Norbert; Wiltfang, Jens

    2016-09-01

    A comprehensive assay validation campaign of a commercially available chemiluminescence multiplex immunoassay for the simultaneous measurement of the amyloid-β peptides Aβ38, Aβ40, and Aβ42 in human cerebrospinal fluid (CSF) is presented. The assay quality parameters we addressed included impact of sample dilution, parallelism, lower limits of detection, lower limits of quantification, intra- and inter-assay repeatability, analytical spike recoveries, and between laboratory reproducibility of the measurements. The assay performed well in our hands and fulfilled a number of predefined acceptance criteria. The CSF levels of Aβ40 and Aβ42 determined in a clinical cohort (n = 203) were statistically significantly correlated with available ELISA data of Aβ1-40 (n = 158) and Aβ1-42 (n = 179) from a different laboratory. However, Bland-Altman method comparison indicated systematic differences between the assays. The data presented here furthermore indicate that the CSF concentration of Aβ40 can surrogate total CSF Aβ and support the hypothesis that the Aβ42/Aβ40 ratio outperforms CSF Aβ42 alone as a biomarker for Alzheimer's disease due to a normalization to total Aβ levels. PMID:27567847

  16. CuO nanosheets-enhanced flow-injection chemiluminescence system for determination of vancomycin in water, pharmaceutical and human serum

    Science.gov (United States)

    Khataee, A. R.; Hasanzadeh, A.; Iranifam, M.; Fathinia, M.; Hanifehpour, Y.; Joo, S. W.

    2014-03-01

    A novel, rapid and sensitive CuO nanosheets (NSs) amplified flow-injection chemiluminescence (CL) system, luminol-H2O2-CuO nanosheets, was developed for determination of the vancomycin hydrochloride for the first time. It was found that vancomycin could efficiently inhibit the CL intensity of luminol-H2O2-CuO nanosheets system in alkaline medium. Under the optimum conditions, the inhibited CL intensity was linearly proportional to the concentration of vancomycin over the ranges of 0.5-18.0 and 18.0-40.0 mg L-1, with a detection limit (3σ) of 0.1 mg L-1. The precision was calculated by analyzing samples containing 5.0 mg L-1 vancomycin (n = 11) and the relative standard deviation (RSD) was 2.8%. Also, a high injection throughput of 120 sample h-1 was obtained. The CuO nanosheets were synthesized by a sonochemical method. Also, X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses were employed to characterize the CuO nanosheets. The method was successfully employed to determine vancomycin hydrochloride in environmental water samples, pharmaceutical formulation and spiked human serum.

  17. Comparison analysis between Western blot method and Magnetic particle Chemiluminescence method detection SLE autoantibody%免疫印迹法和磁微粒化学发光法检测SLE自身抗体的比对分析

    Institute of Scientific and Technical Information of China (English)

    周厚清

    2016-01-01

    目的:比较免疫印迹法(Western blot)和磁微粒化学发光法(Magnetic particle Chemiluminescence method)检测系统性红斑狼疮(SLE)自身抗体的一致性。方法:用免疫印迹法和磁微粒化学发光法对123份血清进行检测,其中系统性红斑狼疮阳性患者63例,健康体检者60例,用卡方检验对两种方法结果进行比较分析。结果:免疫印迹法和磁微粒化学发光法中抗Sm抗体阳性率分别为27.0%和14.3%、抗RNP/Sm阳性率分别为54.0%和42.9%、dsDNA阳性率分别为41.3%和30.2%、抗核小体抗体阳性率分别为31.7%和20.6%、抗核糖体P蛋白抗体阳性率分别为28.6%和11.1%。两种方法的准确性无显著性差异(>0.05)总符合率为80.6%,Kappa值为0.669。结论:免疫印迹法和磁微粒化学发光法检测系统性红斑狼疮自身抗体的检测结果具有可靠性,较好的符合率和准确性,磁微粒化学发光法可以作为简便、快速、经济的检测方法应用于系统性红斑狼疮自身抗体的检测。%Objective: The present study aims to compare Western blot and Magnetic particle Chemiluminescence method consistency on autoimmune antibody detection of systemic lupus erythematosus. Methods: Western blot assay and Magnetic particle Chemiluminescence method were used to detect autoimmune antibody from 123 serum samples, of which 63 patients with systemic lupus erythematosus, the others from 60 healthy persons. The results of the two methods were compared by X2 test. Results: Anti-Sm antibody positive rate of Western blot and Magnetic particle Chemiluminescence method were 27.0 and 14.3 percent respectively. Anti- RNP/Sm antibody positive rates were 39.7% and 42.9% respectively. Anti- ds- DNA antibody positive rates were 41.3% and 30.2% respectively. Anti- nucleosome antibody positive rates were 31.7% and 20.6% respectively. Anti- ribosomal protein antibody positive rates were 28.6% and 11.1% respectively. The accuracy

  18. A hot-spot-active magnetic graphene oxide substrate for microRNA detection based on cascaded chemiluminescence resonance energy transfer

    Science.gov (United States)

    Bi, Sai; Chen, Min; Jia, Xiaoqiang; Dong, Ying

    2015-02-01

    Herein, a cascaded chemiluminescence resonance energy transfer (C-CRET) process was demonstrated from horseradish peroxidase (HRP)-mimicking DNAzyme-catalyzed luminol-H2O2 to fluorescein and further to graphene oxide (GO) when HRP-mimicking DNAzyme/fluorescein was in close proximity to the GO surface. The proposed C-CRET system was successfully implemented to construct three modes of C-CRET hot-spot-active substrates (modes I, II and III) by covalently immobilizing HRP-mimicking DNAzyme/fluorescein-labeled hairpin DNAs (hot-spot-generation probes) on magnetic GO (MGO), resulting in a signal ``off'' state due to the quenching of the luminol/H2O2/HRP-mimicking DNAzyme/fluorescein CRET system by GO. Upon the introduction of microRNA-122 (miRNA-122), the targets (mode I) or the new triggers that were generated through a strand displacement reaction (SDR) initiated by miRNA-122 (modes II and III) hybridized with the loop domains of hairpin probes on MGO to form double-stranded (modes I and II) or triplex-stem structures (mode III), causing an ``open'' configuration of the hairpin probe and a CRET signal ``on'' state, thus achieving sensitive and selective detection of miRNA-122. More importantly, the substrate exhibited excellent controllability, reversibility and reproducibility through SDR and magnetic separation (modes II and III), especially sequence-independence for hairpin probes in mode III, holding great potential for the development of a versatile platform for optical biosensing.Herein, a cascaded chemiluminescence resonance energy transfer (C-CRET) process was demonstrated from horseradish peroxidase (HRP)-mimicking DNAzyme-catalyzed luminol-H2O2 to fluorescein and further to graphene oxide (GO) when HRP-mimicking DNAzyme/fluorescein was in close proximity to the GO surface. The proposed C-CRET system was successfully implemented to construct three modes of C-CRET hot-spot-active substrates (modes I, II and III) by covalently immobilizing HRP-mimicking DNAzyme

  19. Balancing single- and multi-reference correlation in the chemiluminescent reaction of dioxetanone using the anti-Hermitian contracted Schroedinger equation

    International Nuclear Information System (INIS)

    Direct computation of energies and two-electron reduced density matrices (2-RDMs) from the anti-Hermitian contracted Schroedinger equation (ACSE) [D. A. Mazziotti, Phys. Rev. Lett. 97, 143002 (2006)], it is shown, recovers both single- and multi-reference electron correlation in the chemiluminescent reaction of dioxetanone especially in the vicinity of the conical intersection where strong correlation is important. Dioxetanone, the light-producing moiety of firefly luciferin, efficiently converts chemical energy into light by accessing its excited-state surface via a conical intersection. Our previous active-space 2-RDM study of dioxetanone [L. Greenman and D. A. Mazziotti, J. Chem. Phys. 133, 164110 (2010)] concluded that correlating 16 electrons in 13 (active) orbitals is required for realistic surfaces without correlating the remaining (inactive) orbitals. In this paper we pursue two complementary goals: (i) to correlate the inactive orbitals in 2-RDMs along dioxetanone's reaction coordinate and compare these results with those from multireference second-order perturbation theory (MRPT2) and (ii) to assess the size of the active space--the number of correlated electrons and orbitals--required by both MRPT2 and ACSE for accurate energies and surfaces. While MRPT2 recovers very different amounts of correlation with (4,4) and (16,13) active spaces, the ACSE obtains a similar amount of correlation energy with either active space. Nevertheless, subtle differences in excitation energies near the conical intersection suggest that the (16,13) active space is necessary to determine both energetic details and properties. Strong electron correlation is further assessed through several RDM-based metrics including (i) total and relative energies, (ii) the von Neumann entropy based on the 1-electron RDM, as well as the (iii) infinity and (iv) squared Frobenius norms based on the cumulant 2-RDM.

  20. The use of imidazolium ionic liquid/copper complex as novel and green catalyst for chemiluminescent detection of folic acid by Mn-doped ZnS nanocrystals

    Science.gov (United States)

    Azizi, Seyed Naser; Shakeri, Parmis; Chaichi, Mohammad Javad; Bekhradnia, Ahmadreza; Taghavi, Mehdi; Ghaemy, Mousa

    2014-03-01

    A novel chemiluminescence (CL) method using water-soluble Mn-doped ZnS quantum dots (QDs) as CL emitter is proposed for the chemiluminometric determination of folic acid in pharmaceutical formulation. Water-soluble Mn-doped ZnS QDs were synthesized by using L-cysteine as stabilizer in aqueous solutions. The nanoparticles were structurally and optically characterized by X-ray powder diffraction (XRD), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), UV-Vis absorption spectroscopy and photoluminescence (PL) emission spectroscopy. The CL of ZnS QDs induced by directly chemical oxidation and its ionic liquid-sensitized effect in aqueous solution were then investigated. It was found that oxidants, especially hydrogen peroxide, could directly oxidize ZnS QDs to produce weak CL emission in basic conditions. In the presence of 1,3-dipropylimidazolium bromide/copper a drastic light emission enhancement is observed, related to a strong interaction between Cu2+ and the imidazolium ring. Therefore, a new CL analysis system was developed for the determination of folic acid. Under the optimum conditions, there is a good linear relationship between the relative CL intensity and the concentration of folic acid in the range of 1 × 10-9-1 × 10-6 M of folic acid with a correlation coefficient (R2) of 0.9991. The limit of detection of this system was found to be 1 × 10-10 M. This method is not only simple, sensitive and low cost, but also reliable for practical applications.

  1. Determination of phenols by flow injection and liquid chromatography with on-line quinine-sensitized photo-oxidation and quenched luminol chemiluminescence detection

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Wei; Danielson, Neil D

    2003-10-01

    An on-line quinine-sensitized photo-oxidation with quenched chemiluminescence (CL) detection method is developed for phenols using flow injection (FI) and liquid chromatography (LC). This detection method is based on the decrease of light emission from the luminol CL reaction due to the photo-oxidation of phenols that scavenge the photogenerated reactive oxygen species (e.g. singlet oxygen ({sup 1}O{sub 2}) and superoxide (O{sub 2}{center_dot}{sup -})). On-line photo-oxidation is achieved using a coil photo-reactor made from fluoroethylene-propylene copolymer tubing (3048 mmx0.25 mm i.d.) coiled around a mercury UV lamp. A buffer of pH 7 and a concentration of 350 {mu}M for quinine sulfate are determined optimum for the sensitized photo-oxidation. Using a carrier system flow rate of 60 {mu}l/min, calibration curves taken by FI for 10 phenolic compounds in aqueous solutions showed this decreasing sensitivity order: 4-chlorophenol, phenol, 4-nitrophenol, 3-hydroxy-L-kynurenine, 2-nitrophenol, salicylate, 3-nitrophenol, catechol, 2,4-dinitrophenol, and 2,4-dichlorophenol. This detection method using two tandem coil photo-reactors is also applied for the LC separation of phenol, 4-nitrophenol and 4-chlorophenol on an octadecyl (C18) silica LC column using acetonitrile-H{sub 2}O (40:60, v/v) as a mobile phase. The quenched CL detection limits (about 1 {mu}M or 20 pmol) for phenol and 4-chlorophenol are comparable to those for UV detection at 254 nm. Some selectivity in the quenched CL detection is evident by no interference in the FI phenol response even when benzaldehyde and phenethanol concentrations are 8 and 15 times that of phenol.

  2. Chemiluminescence development after initiation of Maillard reaction in aqueous solutions of glycine and glucose: nonlinearity of the process and cooperative properties of the reaction system

    Science.gov (United States)

    Voeikov, Vladimir L.; Naletov, Vladimir I.

    1998-06-01

    Nonenzymatic glycation of free or peptide bound amino acids (Maillard reaction, MR) plays an important role in aging, diabetic complications and atherosclerosis. MR taking place at high temperatures is accompanied by chemiluminescence (CL). Here kinetics of CL development in MR proceeding in model systems at room temperature has been analyzed for the first time. Brief heating of glycine and D-glucose solutions to t greater than 93 degrees Celsius results in their browning and appearance of fluorescencent properties. Developed In solutions rapidly cooled down to 20 degrees Celsius a wave of CL. It reached maximum intensity around 40 min after the reaction mixture heating and cooling it down. CL intensity elevation was accompanied by certain decoloration of the solution. Appearance of light absorbing substances and development of CL depended critically upon the temperature of preincubation (greater than or equal to 93 degrees Celsius), initial pH (greater than or equal to 11,2), sample volume (greater than or equal to 0.5 ml) and reagents concentrations. Dependence of total counts accumulation on a system volume over the critical volume was non-monotonous. After reaching maximum values CL began to decline, though only small part of glucose and glycin had been consumed. Brief heating of such solutions to the critical temperature resulted in emergence of a new CL wave. This procedure could be repeated in one and the same reaction system for several times. Whole CL kinetic curve best fitted to lognormal distribution. Macrokinetic properties of the process are characteristic of chain reactions with delayed branching. Results imply also, that self-organization occurs in this system, and that the course of the process strongly depends upon boundary conditions and periodic interference in its course.

  3. Electrogenerated chemiluminescence biosensing for the detection of prostate PC-3 cancer cells incorporating antibody as capture probe and ruthenium complex-labelled wheat germ agglutinin as signal probe

    International Nuclear Information System (INIS)

    Highlights: • A novel biosensor was developed for the detection of prostate cancer cells. • The selectivity of the biosensor was improved using antibody as capture probe. • The biosensor showed the low extremely detection limit of 2.6 × 102 cells mL−1. • The ruthenium complex-labelled WGA can be transported in the cell vesicles. - Abstract: A highly selective and sensitive electrogenerated chemiluminescence (ECL) biosensor for the detection of prostate PC-3 cancer cells was designed using a prostate specific antibody as a capture probe and ruthenium complex-labelled wheat germ agglutinin as a signal probe. The ECL biosensor was fabricated by covalently immobilising the capture probe on a graphene oxide-coated glassy carbon electrode. Target PC-3 cells were selectively captured on the surface of the biosensor, and then, the signal probe was bound with the captured PC-3 cells to form a sandwich. In the presence of tripropylamine, the ECL intensity of the sandwich biosensor was logarithmically directly proportion to the concentration of PC-3 cells over a range from 7.0 × 102 to 3.0 × 104 cells mL−1, with a detection limit of 2.6 × 102 cells mL−1. The ECL biosensor was also applied to detect prostate specific antigen with a detection limit of 0.1 ng mL−1. The high selectivity of the biosensor was demonstrated in comparison with that of a lectin-based biosensor. The strategy developed in this study may be a promising approach and could be extended to the design of ECL biosensors for highly sensitive and selective detection of other cancer-related cells or cancer biomarkers using different probes

  4. Antioxidant potential of curcumin-related compounds studied by chemiluminescence kinetics, chain-breaking efficiencies, scavenging activity (ORAC and DFT calculations

    Directory of Open Access Journals (Sweden)

    Adriana K. Slavova-Kazakova

    2015-08-01

    Full Text Available This study compares the ability to scavenge different peroxyl radicals and to act as chain-breaking antioxidants of monomers related to curcumin (1: dehydrozingerone (2, zingerone (3, (2Z,5E-ethyl 2-hydroxy-6-(4-hydroxy-3-methoxyphenyl-4-oxohexa-2,5-dienoate (4, ferulic acid (5 and their corresponding C2-symmetric dimers 6–9. Four models were applied: model 1 – chemiluminescence (CL of a hydrocarbon substrate used for determination of the rate constants (kA of the reactions of the antioxidants with peroxyl radicals; model 2 – lipid autoxidation (lipidAO used for assessing the chain-breaking antioxidant efficiency and reactivity; model 3 – oxygen radical absorbance capacity (ORAC, which yields the activity against peroxyl radicals generated by an azoinitiator; model 4 – density functional theory (DFT calculations at UB3LYP/6-31+G(d,p level, applied to explain the structure–activity relationship. Dimers showed 2–2.5-fold higher values of kA than their monomers. Model 2 gives information about the effects of the side chains and revealed much higher antioxidant activity for monomers and dimers with α,β-unsaturated side chains. Curcumin and 6 in fact are dimers of the same monomer 2. We conclude that the type of linkage between the two “halves” by which the molecule is made up does not exert influence on the antioxidant efficiency and reactivity of these two dimers. The dimers and the monomers demonstrated higher activity than Trolox (10 in aqueous medium (model 3. A comparison of the studied compounds with DL-α-tocopherol (11, Trolox and curcumin is made. All dimers are characterized through lower bond dissociation enthalpies (BDEs than their monomers (model 4, which qualitatively supports the experimental results.

  5. Enhancement of on chip chemiluminescence signal intensity of tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system for analysis of chlorpheniramine maleate in pharmaceutical formulations.

    Science.gov (United States)

    Al Lawati, Haider A J; Suliman, Fakhr Eldin O; Al Kindy, Salma M Z; Al-Lawati, Ali M; Varma, Gouri B; Nour, Imad Eldin M

    2010-10-15

    The effect of detection chip geometry on chemiluminescence (CL) signal intensity of tris(1,10-phenanthroline)-ruthenium(II) peroxydisulphate system for analysis of chlorpheniramine maleate (CPM) in pharmaceutical formulations was investigated. It was observed that the design of the detection chip is very crucial and can play an important role in enhancing the CL signal intensity in this system. The CL signal intensity was enhanced 250% when a teardrop micromixer chip was used, compared to the commonly used serpentine chip geometry. The study was conducted using a multi-chip device. In this device, chip 1 was used to prepare and pump the reagent mixture, whereas chip 3 was used for pumping the sample. The two chips were connected to the teardrop chip (2) via silica capillary where detection took place. Non-linear regression curve fitting of the calibration data revealed that the calibration curves are best described by third order polynomial equation with excellent correlation coefficients (R(2)=0.9998) for the concentration range 7.69 × 10(-8) to 5.12 ×1 0(-5)mol L(-1). A linear response is also observed over the range 7.69 × 10(-8) to 1.28 × 10(-5)mol L(-1) (R(2)=0.9996) and the detection limit was found to be 5.49 × 10(-8)mol L(-1). The device was successfully used for the analysis of CPM in tablets and a multi-component cough syrup. Results were reproducible with relative standard deviation (RSD) of 0.6-1.1%. PMID:20875608

  6. Determination of monoamine neurotransmitters and their metabolites in a mouse brain microdialysate by coupling high-performance liquid chromatography with gold nanoparticle-initiated chemiluminescence

    Energy Technology Data Exchange (ETDEWEB)

    Li Na; Guo Jizhao; Liu Bo; Yu Yuqi [Department of Chemistry, University of Science and Technology of China (USTC), JinZhai Road No: 96, 230026 Hefei, Anhui (China); Cui Hua, E-mail: hcui@ustc.edu.cn [Department of Chemistry, University of Science and Technology of China (USTC), JinZhai Road No: 96, 230026 Hefei, Anhui (China); Mao Lanqun; Lin Yuqing [Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences (CAS), 100080 Beijing (China)

    2009-07-10

    Our previous work showed that gold nanoparticles could trigger chemiluminescence (CL) between luminol and AgNO{sub 3}. In the present work, the effect of some biologically important reductive compounds, including monoamine neurotransmitters and their metabolites, reductive amino acids, ascorbic acid, uric acid, and glutathione, on the novel CL reaction were investigated for analytical purpose. It was found that all of them could inhibit the CL from the luminol-AgNO{sub 3}-Au colloid system. Among them, monoamine neurotransmitters and their metabolites exhibited strong inhibition effect. Taking dopamine as a model compound, the CL mechanism was studied by measuring absorption spectra during the CL reaction and the reaction kinetics via stopped-flow technique. The CL inhibition mechanism is proposed to be due to that these tested compounds competed with luminol for AgNO{sub 3} to inhibit the formation of luminol radicals and to accelerate deposition of Ag atoms on surface of gold nanoparticles, leading to a decrease in CL intensity. Based on the inhibited CL, a novel method for simultaneous determination of monoamine neurotransmitters and their metabolites was developed by coupling high-performance liquid chromatography with this CL reaction. The new method was successfully applied to determine the compounds in a mouse brain microdialysate. Compared with the reported HPLC-CL methods, the proposed method is simple, fast, and could determine more analytes. Moreover, the limits of linear ranges for NE, E, and DA using the proposed method were one order of magnitude lower than the luminol system without gold nanoparticles.

  7. 流动注射-化学发光法测定头孢氨苄%Flow Injection/Chemiluminescence Determination of Cephalexin

    Institute of Scientific and Technical Information of China (English)

    范顺利; 吕超; 王军; 周庆祥

    2001-01-01

    头孢氨苄在 H2SO4 溶液中降解后,其产物可在酸性条件下与 Ce(Ⅳ) 产生化学发光反应,罗丹明6G 对该反应有较强的增敏作用。据此建立了流动注射-化学发光法测定头孢氨苄的新方法。该方法的线性范围为 0.10~10.0 mg/L 检出限为 0.06 mg/L,相对标准偏差 (n=11,c=1.00 mg/L) 为 0.8%。方法用于药物中头孢氨苄含量的测定,其结果与标准方法一致,回收率为 96%~106%。%A new flow-injection chemiluminescence(CL) method for determination of cephalexin was described. The method was based on CL reaction of the product degraded from cephalexin in sulphuric acid medium with Ce(Ⅳ) in acidic condition sensitized by rhodamine 6G. The linear range of the method is 0.10~10.0 mg/L cephalexin with detection limit of 0.06 mg/L and relative standard deviation(n=11,c=1.00 mg/L) of 0.8%. The method was applied to determination of cephalexin in pharmaceutical preparations with recovery of between 96% and 106%.

  8. Electrogenerated chemiluminescence biosensing for the detection of prostate PC-3 cancer cells incorporating antibody as capture probe and ruthenium complex-labelled wheat germ agglutinin as signal probe

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Haiying [Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062 (China); Department of Chemistry, Yuncheng University, Yuncheng 044300 (China); Li, Zhejian; Shan, Meng; Li, Congcong; Qi, Honglan; Gao, Qiang [Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062 (China); Wang, Jinyi [College of Science and College of Veterinary Medicine, Northwest A& F University, Yangling 712100 (China); Zhang, Chengxiao, E-mail: cxzhang@snnu.edu.cn [Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi’an 710062 (China)

    2015-03-10

    Highlights: • A novel biosensor was developed for the detection of prostate cancer cells. • The selectivity of the biosensor was improved using antibody as capture probe. • The biosensor showed the low extremely detection limit of 2.6 × 10{sup 2} cells mL{sup −1}. • The ruthenium complex-labelled WGA can be transported in the cell vesicles. - Abstract: A highly selective and sensitive electrogenerated chemiluminescence (ECL) biosensor for the detection of prostate PC-3 cancer cells was designed using a prostate specific antibody as a capture probe and ruthenium complex-labelled wheat germ agglutinin as a signal probe. The ECL biosensor was fabricated by covalently immobilising the capture probe on a graphene oxide-coated glassy carbon electrode. Target PC-3 cells were selectively captured on the surface of the biosensor, and then, the signal probe was bound with the captured PC-3 cells to form a sandwich. In the presence of tripropylamine, the ECL intensity of the sandwich biosensor was logarithmically directly proportion to the concentration of PC-3 cells over a range from 7.0 × 10{sup 2} to 3.0 × 10{sup 4} cells mL{sup −1}, with a detection limit of 2.6 × 10{sup 2} cells mL{sup −1}. The ECL biosensor was also applied to detect prostate specific antigen with a detection limit of 0.1 ng mL{sup −1}. The high selectivity of the biosensor was demonstrated in comparison with that of a lectin-based biosensor. The strategy developed in this study may be a promising approach and could be extended to the design of ECL biosensors for highly sensitive and selective detection of other cancer-related cells or cancer biomarkers using different probes.

  9. Determination of Vitamin B6 by Flow Injection Chemiluminescence Method%流动注射化学发光法测定维生素B6

    Institute of Scientific and Technical Information of China (English)

    薛元英; 揭永文; 陈平清

    2009-01-01

    基于维生素B6对鲁米诺-过氧化氢化学发光反应的增敏作用,结合流动注射技术,建立了流动注射化学发光反应测定维生素B6的新方法.该方法的线性范围为5.0×10-2.0×10-6 g/mL,检出限为2.3×10-8g/mL.对5.0×10-6g/mL的维生素B6进行11次平行测定,相对标准偏差为2.8%.实验表明该方法简单、快速、灵敏.%A new flow-injection chemiluminescence method for the determination of Vitamin B6 was developed.It is based on the catalysis of Vitamin B6 to chemilumincecrce reaction between luminol and hydrogen peroxide.The linear range extends from 5.0×10-8 to 2.0×10-6 g/mL and the detection limit is 1.3×10-8 g/mL.The relative standard deviation (RSD) is 2.4% for 5.0× 10-6 g/mL Vitamin B6 solution in eleven repeated measurements.The method has the advantages of simplicity,rapidity and sensitrvity.

  10. Determination of Glutamic Acid by Potassium Permanganate-glyoxal Chemiluminescence System%高锰酸钾-乙二醛化学发光体系测定谷氨酸

    Institute of Scientific and Technical Information of China (English)

    樊雪梅; 王书民

    2014-01-01

    !押建立了测定谷氨酸的化学发光新方法,确定了该方法的测定最佳条件,并研究了抑制体系的机理。在最佳条件下,谷氨酸浓度在2.0×10-8-5.0×10-5 mol·L-1范围内与相对发光强度成正比,方法的检出限为6.0×10-9 mol·L-1,对1.0×10-6 mol·L-1的谷氨酸平行测定9次,相对标准偏差为2.5%。该法用于味精产品中谷氨酸含量分析。%A novel flow injection chemiluminescence method was developed for the determination of glutamic acid, based on the inhibited effect of glutamic acid on the chemiluminescence reaction of potassium permanganate and glyoxal in the acidic medium. The optimum conditions and the possible inhibitory mechanism was also discussed. Under optimum conditions, the relative chemiluminescence intensity was 1inearly related to the concentration of glutamic acid in the range of 2.0×10-8-5.0×10-5 mol·L-1 with a detection limit of 6.0×10-9 mol·L-1, the RSD for measurement of 1.0×10-6 mol·L-1 glutamic acid (n=9) is 2.5%. This method was applied to the determination of glutamic acid in monosodium glutamate samples with satisfatory results.

  11. Research progress on chemiluminescence immunoassay technique and its application in food safety detection%化学发光免疫分析技术及其在食品安全检测中的研究进展

    Institute of Scientific and Technical Information of China (English)

    张燕; 杨金易; 曾道平; 易娜; 孙远明

    2013-01-01

    Chemiluminescence immunoassay (CLIA) received more and more attention in recent years for its high sensitivity, wide linear range, simplicity, feasibility and non-isotopic contamination. The classification and principle of chemiluminescence immunoassay were introduced briefly in this article, and the CLIAs were classified systematically according to different marker substances of the chemiluminescence system and the difference of detection systems. A variety of methods were also introduced in detail. Moreover, its advantages and disadvantages were analyzed and the application of CLIA in food safety detection, including the detection of microorganism, toxins, pesticide residues, animal drug residues and the genetically modified food were dis-cussed. The future development of CLIA was also prospected.%近年来,化学发光免疫分析因其具有特异性强、灵敏度高、线性范围宽、仪器简单、操作方便、无放射性污染等优点越来越受到人们的关注。本文简要介绍了化学发光免疫分析的类型及其原理,根据化学发光体系中标记物质的不同以及检测体系的差别,对化学发光免疫分析技术进行了系统的分类,并且对各种方法进行了详细的介绍,对其优点和不足做了透彻的分析。对该分析方法在食品安全检测中的运用,包括检测食品中微生物、毒素、农药残留、兽药残留等进行了综述,并展望其发展方向。

  12. Latest progress of chemiluminescent immunoassay in food safety detection%化学发光免疫分析方法在食品安全检测中的研究进展

    Institute of Scientific and Technical Information of China (English)

    金茂俊; 王静; 杨丽华; 杜鹏飞; 邵华; 金芬; 王珊珊; 佘永新

    2014-01-01

    Chemiluminescent immunoassay (CLIA) as the technology with high specificity and sensitivity, after decades of development, has got a good application in the field of food safety detection. According to the different labelled substances used in the CLIA system, the methods were divided into CLIA, chemiluminescent enzyme immunoassay (CLEIA) and electrochemiluminescent immunoassay (ECLIA). This review briefly de-scribed the classical chemiluminescent labelled substances and the principles of various CLIA systems. Mean-while, the application of CLIA in the detection of pesticide residue, veterinary drug residue, biological toxins, prohibited addictives, and microorganisms were introduced. Accompanied with the further development of CLIA applied in novel chemiluminescent labelled substances and sensitized system, the technology will have a prosperous future in detection of food safety.%化学发光免疫分析方法作为一项具有高特异性和高灵敏度的免疫分析方法,经过近几十年的发展,目前已在食品安全检测领域得到了良好的应用。本文根据不同化学发光免疫分析方法所选择标记物的不同,将化学发光免疫分析方法分为化学发光免疫分析、化学发光酶免疫分析和电化学发光免疫分析三个类别,并对各方法常用的化学发光标记物及分析方法原理进行介绍。同时,综述了化学发光免疫分析方法在农兽药残留、生物毒素、违禁添加物及微生物检测方面的应用进展情况。随着化学发光免疫分析方法在新型化学发光标记物及化学发光增敏体系方面取得进一步的研究进展,其在食品安全检测领域必将得到更为广泛的应用。

  13. A novel chemiluminescence method for determination of bisphenol Abased on the carbon dot-enhanced HCO{sub 3}{sup −}–H{sub 2}O{sub 2} system

    Energy Technology Data Exchange (ETDEWEB)

    Amjadi, Mohammad, E-mail: amjadi@tabrizu.ac.ir; Manzoori, Jamshid L.; Hallaj, Tooba

    2015-02-15

    A simple and sensitive chemiluminescence (CL) method on the basis of carbon dot (C-dot) enhanced HCO{sub 3}{sup −}–H{sub 2}O{sub 2} system, is designed for the determination of bisphenol A (BPA). The very weak CL of the HCO{sub 3}{sup −}–H{sub 2}O{sub 2} system is enhanced by a factor of ∼100 in the presence of C-dots. Possible mechanisms that lead to the effect were elucidated by recording fluorescence and CL spectra and studying the effect of some radical scavengers. This enhancement is inhibited by BPA in the concentration range from 1.0 to 100 µg L{sup −1}. This is exploited for its trace determination with a detection limit (3 s) of 0.3 µg L{sup −1}. The established method was applied to the determination of BPA in baby bottle and water samples with satisfactory results. - Highlights: • The effect of carbon dots on HCO{sub 3}{sup −}–H{sub 2}O{sub 2} chemiluminescence reaction is studied. • Carbon dots greatly enhance the CL signal of this reaction (∼100 fold). • The new CL system was applied to determination of bisphenol A in real samples.

  14. Rapid Determination of Sodium Saccharin by FI-Chemiluminescence%流动注射-化学发光法快速测定糖精钠

    Institute of Scientific and Technical Information of China (English)

    高向阳; 魏姜勉; 王珊; 尹中瑞

    2011-01-01

    FI-Chemiluminescence determination of sodium saccharin based on the CL reaction between luminol and H2O2 was reported.In an acid medium,the solution of sodium saccharin was reacted with K2Cr2O7 to reduce Cr(Ⅵ) quantitatively to Cr(Ⅲ) and the CL intensity of the reaction system was remarkably enhanced by the Cr(Ⅲ) formed.The content of Cr(Ⅲ) was then determined and converted into contents of sodium saccharin.Linear relationship between values of increase in CL intensity and concentration of sodium saccharin was obtained in the range of 5.0×10-7-1.0×10-5mol·L-1.Detection limit(3s) was found to be 1.08×10-11mol·L-1.Recovery was tested by addition of standard solutions at 3 concentration levels to sample solutions,values of recovery obtained were in the range of 95.6%-105.8% with RSD(n=12) of 2.9%.%提出了基于鲁米诺与过氧化氢化学发光反应的流动注射-化学发光法快速测定糖精钠的方法。在酸性介质中,糖精钠与重铬酸钾反应定量生成铬(Ⅲ),所得的铬(Ⅲ)可显著增加上述化学发光反应的化学发光强度,且其强度的增加值与糖精钠浓度在5.0×10-7~1.0×10-5 mol.L-1范围内呈线性关系,方法的检出限(3s)为1.08×10-11 mol.L-1。在试样溶液中分别加入3个浓度水平的糖精钠标准溶液,按方法测定求得其回收率在95.6%~105.8%之间,相对标准偏差(n=12)为2.9%。

  15. FI-Inhibitory Chemiluminescence Determination of Carbofuran%流动注射-抑制化学发光法测定克百威

    Institute of Scientific and Technical Information of China (English)

    贾风燕; 刘振波; 王荣; 刘永明

    2011-01-01

    Based on the inhibitory effect of carbofuran on the CL-reaction system of luminol-H2O2-sodium copper chlorophyll in an alkaline medium, a method for the determination of carbofuran by FI-inhibitory chemiluminescence (CL) was proposed. It was found that a stable complex was formed by the reaction of sodium copper chlorophyll with carbofuran in the mole ratio of 1 to 1, the process of quenching of fluorescence of carbofuran was proved to be a process of static quenching. Equilibrium constant (K0) and combination distance (r) the complex were determined and values obtained were 3. 41 × 105L · mol-1 and 0. 39 nm respectively. Linear relationship was found between values of CL intensity and mass concentration of carbofuran in the range of 0. 08 to 2. 0 mg · L-1,and detection limit (30/k) of the method found was 0. 03 mg · L-1 The proposed method was used in analysis of carbofuran in secticide samples, giving values of average recovery of 101.5%.%基于在碱性介质中,克百威抑制鲁米诺-过氧化氢-叶绿素铜钠体系的化学发光,提出了流动注射-抑制化学发光法测定克百威含量的方法.试验结果表明:叶绿素铜钠对克百威荧光猝灭过程是静态猝灭过程,叶绿素铜钠与克百威结合形成物质的量比为1比1的稳定配合物,平衡常数(K0)为3.41×105L·mol-1(25℃),结合距离(r)为0.39nm.克百威质量浓度在0.08~2.00 mg·L-1范围内与其发光强度呈线性关系,方法检出限(3σ/k)为0.03 mg·L-1.此法用于克百威杀虫剂样品的分析,测得方法的平均加标回收率为101.5%.

  16. Development of a single-chain variable fragment-alkaline phosphatase fusion protein and a sensitive direct competitive chemiluminescent enzyme immunoassay for detection of ractopamine in pork

    Energy Technology Data Exchange (ETDEWEB)

    Dong Jiexian; Li Zhenfeng; Lei Hongtao; Sun Yuanming [Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642 (China); Ducancel, Frederic [CEA, iBiTec-S, Service de Pharmacologie et d' Immnoanalyse (SPI), CEA Saclay, F-91191 Gif sur Yvette (France); Xu Zhenlin [Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642 (China); Boulain, Jean-Claude [CEA, iBiTec-S, Service de Pharmacologie et d' Immnoanalyse (SPI), CEA Saclay, F-91191 Gif sur Yvette (France); Yang Jinyi; Shen Yudong [Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642 (China); Wang Hong, E-mail: gzwhongd@63.com [Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642 (China)

    2012-07-29

    Graphical abstract: Detection model of dc-CLEIA based on anti-RAC scFv-AP fusion protein. Highlights: Black-Right-Pointing-Pointer The scFv-AP fusion protein against ractopamine (RAC) was produced. Black-Right-Pointing-Pointer A dc-CLEIA for RAC was developed based on the purified scFv-AP fusion protein. Black-Right-Pointing-Pointer The sensitivity of dc-CLEIA was 10 times as sensitive as dc-ELISA for RAC. Black-Right-Pointing-Pointer Recovery tests from pork samples were studied. Black-Right-Pointing-Pointer Good accuracy was obtained. - Abstract: A rapid, sensitive chemiluminescent enzyme immunoassay (CLEIA) for ractopamine (RAC) based on a single-chain variable fragment (scFv)-alkaline phosphatase (AP) fusion protein was developed. The scFv gene was prepared by cloning the heavy- and light-chain variable region genes (V{sub H} and V{sub L}) from hybridoma cell line AC2, which secretes antibodies against RAC, and assembling V{sub H} and V{sub L} genes with a linker by means of splicing overlap extension polymerase chain reaction. The resulting scFv gene was inserted into the expression vector pLIP6/GN containing AP to produce the fusion protein in Escherichia coli strain BL21. The purified scFv-AP fusion protein was used to develop a direct competitive CLEIA (dcCLEIA) protocol for detection of RAC. The average concentration required for 50% inhibition of binding and the limit of detection of the assay were 0.25 {+-} 0.03 and 0.02 {+-} 0.004 ng mL{sup -1}, respectively, and the linear response range extended from 0.05 to 1.45 ng mL{sup -1}. The assay was 10 times as sensitive as the corresponding enzyme-linked immunosorbent assay based on the same fusion protein. Cross-reactivity studies showed that the fusion protein did not cross react with RAC analogs. DcCLEIA was used to analyze RAC spiked pork samples, and the validation was confirmed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS). The results showed a good correlation between

  17. Label-free and amplified electrogenerated chemiluminescence biosensing method for the determination of DNA methyltransferase activity using signal reagent-assembled graphene oxide

    International Nuclear Information System (INIS)

    Highlights: • Label-free ECL biosensing method for DNA MTase was developed. • The ECL biosensing method is based on Ru(phen)32+-assembled graphene oxide served as an ECL signal compound. • The biosensing method showed high sensitivity. - Abstract: A novel label-free electrogenerated chemiluminescence (ECL) biosensing method for the determination of DNA methyltransferase (MTase) activity was developed on base of enzyme-linkage reactions and tris(1, 10-phenanthroline) ruthenium-assembled graphene oxide (GO) served as an ECL signal compound. The ECL biosensing electrode was fabricated by self-assembling 5′-thiol modified hairpin-capture DNA probe containing methylation recognition site 5′-GATC-3′ on the surface of a gold electrode. When DNA adenine methylation (Dam) MTase and S-adenosyl-L-methionine were introduced, all adenine residues within 5′-GATC-3′ of hairpin-capture DNA probe on the biosensing electrode were methylated. After the methylated biosensing electrode was treated by the methylation-sensitive restriction endonuclease Dpn I, the methylated adenines were cleaved, methylation-induced scission of hairpin-capture DNA probe would displace the hairpin section and remain the “capture DNA probe” section on the gold electrode, then a long ssDNA was immobilized via the partial hybridization reaction between long ssDNA and hairpin-capture DNA probe remained section, the more binding site allow tris(1, 10-phenanthroline) ruthenium-assembled GO to be more bound to the long ssDNA on the electrode surface through both hydrophobic and π–π stacking interaction, in conjunction with the generation of a increased ECL signal. The ECL intensity versus the concentration of Dam MTase was linear in the range from 0.02 unit/mL to 10 unit/mL. The detection limit was 0.01 unit/mL. This work demonstrates that using the different affinities of GO for ssDNA and dsDNA for the fabrication of the label-free ECL biosensing method for DNA MTase activity is

  18. Application of a newly developed high-sensitivity HBsAg chemiluminescent enzyme immunoassay for hepatitis B patients with HBsAg seroclearance.

    Science.gov (United States)

    Shinkai, Noboru; Matsuura, Kentaro; Sugauchi, Fuminaka; Watanabe, Tsunamasa; Murakami, Shuko; Iio, Etsuko; Ogawa, Shintaro; Nojiri, Shunsuke; Joh, Takashi; Tanaka, Yasuhito

    2013-11-01

    We modified and automated a highly sensitive chemiluminescent enzyme immunoassay (CLEIA) for surface antigen (HBsAg) detection using a combination of monoclonal antibodies, each for a specific epitope of HBsAg, and by improving an earlier conjugation technique. Of 471 hepatitis B virus (HBV) carriers seen in our hospital between 2009 and 2012, 26 were HBsAg seronegative as determined by the Abbott Architect assay. The Lumipulse HBsAg-HQ assay was used to recheck those 26 patients who demonstrated seroclearance by the Abbott Architect assay. The performance of the Lumipulse HBsAg-HQ assay was compared with that of a quantitative HBsAg detection system (Abbott Architect) and the Roche Cobas TaqMan HBV DNA assay (CTM) (lower limit of detection, 2.1 log copies/ml) using blood serum samples from patients who were determined to be HBsAg seronegative by the Abbott Architect assay. Ten patients had spontaneous HBsAg loss. Of 8 patients treated with nucleotide analogues (NAs), two were HBsAg seronegative after stopping lamivudine therapy and 6 were HBsAg seronegative during entecavir therapy. Eight acute hepatitis B (AH) patients became HBsAg seronegative. Of the 26 patients, 16 were HBsAg positive by the Lumipulse HBsAg-HQ assay but negative by the Abbott Architect assay. The differences between the two assays in terms of detectable HBsAg persisted over the long term in the spontaneous loss group (median, 10 months), the NA-treated group (2.5 months), and the AH group (0.5 months). In 9 patients, the Lumipulse HBsAg-HQ assay detected HBsAg when HBV DNA was negative by the CTM assay. HBsAg was also detected by the Lumipulse HBsAg-HQ assay in 4 patients with an anti-HBs concentration of >10 mIU/ml, 3 of whom had no HBsAg escape mutations. The automatic, highly sensitive HBsAg CLEIA Lumipulse HBsAg-HQ is a convenient and precise assay for HBV monitoring.

  19. Hydroxyl radical-related electrogenerated chemiluminescence reaction for a ruthenium tris(2,2')bipyridyl/co-reactants system at boron-doped diamond electrodes

    Energy Technology Data Exchange (ETDEWEB)

    Honda, K. [Department of Chemistry and Earth Sciences, Faculty of Science Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8512 (Japan)]. E-mail: khonda@yamaguchi-u.ac.jp; Yamaguchi, Y. [Department of Chemistry and Earth Sciences, Faculty of Science Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8512 (Japan); Yamanaka, Y. [Department of Chemistry and Earth Sciences, Faculty of Science Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8512 (Japan); Yoshimatsu, M. [Department of Chemistry and Earth Sciences, Faculty of Science Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8512 (Japan); Fukuda, Y. [Department of Chemistry and Earth Sciences, Faculty of Science Yamaguchi University, 1677-1 Yoshida, Yamaguchi-shi, Yamaguchi 753-8512 (Japan); Fujishima, A. [Kanagawa Academy of Science and Technology (KAST), 3-2-1, Sakato, Takastu-ku, Kawasaki-shi, Kanagawa 213-0012 (Japan)

    2005-11-01

    An electrogenerated chemiluminescence (ECL) reaction of the Ru(bpy){sub 3} {sup 2+} (2,2'-bipyridyl, bpy)/co-reactant system in the extremely high-potential region (over 2.6 V versus Ag/AgCl) was probed using a boron-doped diamond (BDD) electrode. At the BDD electrode, three ECL waves (1.25, 2.30 and 3.72 V) were observed in cyclic voltammograms for 20 mM ascorbic acid (AA). For the ECL peaks observed at 1.25 V corresponding to the oxidation potential for Ru(bpy){sub 3} {sup 2+} (1.15 V), the light intensities and current densities were found to depend on the square root of the AA concentration. This suggests that AA oxidation, followed by the formation of the reducing radical that is necessary for generating the excited state of Ru(bpy){sub 3} {sup 2+*} occurred through homogeneous electron-transfer between Ru(bpy){sub 3} {sup 3+} and the AA species. However, for the ECL peaks at 2.30 V, the current densities and light intensities linearly increased with increasing AA concentration, suggesting that the reducing radical was formed through the direct oxidation at the electrode surface. The ECL reaction at 3.72 V was observed only at the BDD electrode and not at other electrodes. The onset potentials for the light intensity were approximately 2.6 V, independently of the type of the co-reactants (e.g. 2-propanol and AA). The peak potentials exhibited linear relation with the co-reactant concentration. In the analysis of the ECL intensity for various co-reactants (alcohols) that show different reactivity for the hydrogen abstraction reaction, the order of the light intensities at the peaks for alcohols was found to be consistent with that for the rate constants of the hydrogen abstraction reaction. These results indicate that the co-reactant radical was formed through the hydrogen abstraction reaction with the hydroxyl radical (HO{center_dot}) generated during the oxygen evolution reaction.

  20. 鲁米诺-高碘酸钾化学发光法测定盐酸阿朴吗啡%Flow injection chemiluminescence method for determination of apomorphini hydrochloridum

    Institute of Scientific and Technical Information of China (English)

    李世君; 杨冉; 屈凌波; 李建军

    2011-01-01

    目的:在碱性条件下盐酸阿朴吗啡(Apomorphini Hydrochloridum)对luminol-KIO化学发光体系具有很强的增敏作用,据此建立了流动注射化学发光法测定盐酸阿朴吗啡新方法.方法:线性范围为5.5×10~0.1 mg·L,检出限为4.4×10mg·L,RSD(n=10,c=0.02 mg·L)为2.6%,结果:已用于阿朴吗啡在尿样和血样中含量的测定,尿样中的加标回收率为97.32%~104.69%,血样中加标回收率为106.30%~110.57%.结论:由于其易见光分解,需使用合理的前处理手段.%Objective: A novel chemiluminescence method for the determination of apomorphini hydrochloridum was developed. It was based on the fact that Apomorphini Hydrochloridum could enhance the chemiluminescence intensity from the reaction of luminol with potassium periodate in alkaline medium. Method:The relationship between the enhanced intensity of chemiluminescence and the concentration of apomorphini hydrochlori - dum was linear in the range of 5. 5 × 10-3 -0. 1 mg ? L-1 and the detection limit was 4. 4 × 10-3 mg ? L-1. The RSD was 2. 58% (re = 10, c = 0. 02 mg ? L-1). Results; The method has been successfully applied to the determination of apomorphine hydrochloridum in urine sample and blood sample with the recoveries of 97. 32% - 104. 69% and 106. 30% -110. 57%. Conclusion;A reasonable means of pre -treatment was needed because of it is easy to decomposition in the light.

  1. 高锰酸钾-鲁米诺化学发光体系测定香草醛%Determination of vanillin with potassium permanganate-luminol chemiluminescence system

    Institute of Scientific and Technical Information of China (English)

    周艳梅; 王亚萍; 马同森; 谢晓龙

    2012-01-01

    Based on the fact that the chemiluminescence (CL) reaction of potassium permanganate and luminol in alkaline medium could be enhanced by vanillin, a new analytical method of chemiluminescence to detect vanillin was developed, which expands the application of flow-injection chemiluminescence in food analysis. The effect of type of medium and concentration of reagents on CL intensity was investigated. The results showed that the optimum concentrations of NaOH, KMnO4 and Lumino were 0.20 mol/L, 3. 0 × 10 -5 mol/L and 7. 0 × 10-5 mol/L, respectively. Under the optimum conditions, linearity relationship of values of CL intensity and mass concentration of vanillin was obtained in the range of 5. 0× 10-8 ~3. 0 × 10-6g/mL, with a equation of linear regression as A/ = 7. 188 3 × 108 C( g/mL) + 71. 767 4 (r = 0.994 2). Parallel determination of 5. 0 × 10-7 g/mL vanillin for 11 times show that the RSD is 1.3% with a detection limit of 1.1 × 10-8 g/mL. A new method of flow-injection chemiluminescence to detect vanillin was found, which has been successfully applied to the determination of vanillin in pill and the recovery rate obtained were in the range of 98% -104%.%为了拓展流动注射化学发光法在食品检测中的应用,利用草醛能够增敏NaOH介质中高锰酸钾—鲁米诺化学发光体系的发光信号的特点,建立了化学发光测定香草醛的方法.文中考察了介质浓度、发光试剂浓度等因素对发光体系信号的影响,确定了测定香草醛的最佳条件:介质NaOH浓度为0.20 mol/L,高锰酸钾浓度为3.0×10-5 mol/L,鲁米诺浓度为7.0×10-5mol/L.在优化实验条件下,体系的发光强度与香草醛浓度在5.0×10-8~3.0×10-6g/mL内呈线性关系,标准曲线:△I=7.1883 ×108C(g/mL) +71.767 4(r =0.994 2).对5.0×10-7 g/mL的香草醛平行测定11次,相对偏差为1.3%,其检出限为1.1 × 10-8g/mL.建立了测定香草醛的流动注射化学发光法新方法,并成功地测定了片剂中香

  2. Chemiluminescence of carbon dots induced by diperiodato-nicklate (IV) in alkaline solution and its application to a quenchometric flow-injection assays of paracetamole, L-cysteine and glutathione

    International Nuclear Information System (INIS)

    Aqueous solutions of carbon dots (C-dots) were prepared by microwave-assisted thermal carbonization of poly(ethylene glycol). They were investigated by transmission electron microscopy, absorption and fluorescence spectra. It is shown that diperiodato-nicklate(IV), a strong oxidant, induces the chemiluminescence (CL) of C-dots in strongly alkaline solution without use of an additional reagent. A mechanism for this reaction is suggested. It is also found that the CL of the system is quenched by paracetamole, L-cysteine and glutathione. Under the optimized conditions, the calibration plot is linear with a correlation coefficient (r) of >0.995. The limits of detection are 90, 8, and 60 µg L-1 for paracetamole, L-cysteine, and glutathione, respectively. Spiked urine and serum samples were analyzed and gave recoveries in the range from 84.38 to 116.0 %, with an RSD of 1.2–2.7 %. (author)

  3. Comparative study of ß-glucan induced respiratory burst measured by nitroblue tetrazolium assay and real-time luminol-enhanced chemiluminescence assay in common carp (Cyprinus carpio L.)

    DEFF Research Database (Denmark)

    Jiménez, Natalia Ivonne Vera; Pietretti, D.; Wiegertjes, G. F.;

    2013-01-01

    in the clearance of pathogens and tissue regeneration processes. Therefore, the respiratory burst and associated ROS constitute important indicators of fish health status. This paper compares two methods for quantitation of ROS produced during the respiratory burst in common carp: the widely used, single......The respiratory burst is an important feature of the immune system. The increase in cellular oxygen uptake that marks the initiation of the respiratory burst is followed by the production of reactive oxygen species (ROS) such as superoxide anion and hydrogen peroxide which plays a role...... kidney cells of carp. However, whereas the NBT assay was shown to detect the production of only superoxide anions, the real-time luminol-enhanced assay could detect the production of both superoxide anions and hydrogen peroxide. Only the chemiluminescence assay could reliably record the production of ROS...

  4. Determination of p-phenylenediamine content in hair dye by flow injection analysis of chemiluminescence intensity inhibition%流动注射化学发光抑制法测定染发剂中对苯二胺

    Institute of Scientific and Technical Information of China (English)

    付小宁

    2011-01-01

    Through the study on chemiluminescence intensity inhibition effect of p -phenylenediamine on Na2CO3 -NaNO2 -H2O2 system,a flow injection analysis method for determination of p- phenylenediamine content in hair dye product was established. Experimental work results showed that, under the selected optimal conditions, the p - phenylenediamine solution mass concentration and the reduction of the value of the chemiluminescence intensity displays good linear relationship. In eleven parallel tests carried out with p-phenylenediamine solution of mass concentration of 1.0 × 10-4 g · L-1, the relative standard deviation is 0. 58% ,and the detection limit is 1.5 × 10-7 g· L-1.%通过对酸性介质中对苯二胺抑制Na2CO3-NaNO2-H2O2体系的化学发光进行研究,建立了一种流动注射-抑制化学发光测定染发剂中对苯二胺的方法.实验结果表明,在所选择的优化条件下,对苯二胺溶液的质量浓度与化学发光强度的降低值呈良好的线性关系,对质量浓度为1.0×10-4g·L-1的对苯二胺溶液进行11次平行测定的相对标准偏差为0.58%,检出限为1.5×10-7g·L-1.

  5. The fit of four parameter logistic calibration of DXI 800 chemiluminescence analyzer by Origin software%采用Origin软件计算DXI 800发光仪的四参数logistic定标数据

    Institute of Scientific and Technical Information of China (English)

    王强; 马小红; 翁秀妹

    2014-01-01

    Objective To fit 4 parameter logistic calibration of DXI 800 chemiluminescence analyzer by Origin software and compare the datum difference between Origin and DXI 800,and to investigate the calculation possibility of unknown results of DXI 800 by Origin software.Methods The calibration results of testosterone,total thyroxine, estradiol,troponin I,thyrotropic hormone,carcino-embryonic antigen and progesterone were collected and fit by Origin software,and the calculation results with the original results of DXI 800 system were analyzed comparatively.Results The calculation results of Origin software were very close to the original results of DXI 800,and mean bias was from-0.85% to 1 .68%.Conclusions Origin software is suitable for the calculation of unknown results of DXI 800 chemiluminescence analyzer.%目的:采用Origin软件拟合DXI 800发光仪的四参数logistic定标数据,比较其与DXI 800计算的数据差异,探讨采用Origin软件计算DXI 800未知数据的可能性。方法选择睾酮、总甲状腺素、雌二醇、肌钙蛋白I、促甲状腺激素、癌胚抗原和孕酮的定标数据,采用Origin软件拟合四参数logistic计算结果,与DXI 800发光仪计算的结果比较。结果采用 Origin 软件计算的结果与 DXI 800计算的结果基本一致,平均偏倚介于-0.85%~1.68%之间。结论可以采用Origin软件计算DXI 800发光仪的未知结果。

  6. L-酪氨酸-高锰酸钾-硫酸化学发光体系研究%Chemiluminescent System of L-Tyrosine-Potassium Permanganate-Sulfuric Acid

    Institute of Scientific and Technical Information of China (English)

    朱昌青; 李永新; 王伦

    2001-01-01

    The reaction between L-tyrosine and potassium permanganate in thesufuric acid medium gave intensive chemiluminescence. A new analytical method for determination of L-tyrosine was proposed.The linear relationship between the intensity of chemiluminescence and the concentration of L-tyrosine is in the range of 4.0×10-6~2.8×10-5 mol/L with detection limit of 8.8×10-7 mol/L. The RSD for measurement of 1.0×10-5 mol/L L-tyrosine (n=10) is 2.7 %. The method was applied to the determination of L-tyrosine in injection of amino-acid with satisfactory results.%在硫酸介质中,L-酪氨酸与高锰酸钾反应能产生较强的化学发光,据此建立了测定L-酪氨酸的分析方法。该法线性范围为4.0×10-6~2.8×10-5mol/L;检出限为8.8×10-7mol/L;对1.0×10-5mol/L的L-酪氨酸进行连续10次平行测定,相对标准偏差为2.7%。该法已应用于测定医用氨基酸注射液中的L-酪氨酸。

  7. Detection of serum HIV -1/HIV- 2 antibody, syphilis antibody and hepatitis C antibody by ELISA and chemiluminescence assay%探讨ELISA和化学发光法对血清中HIV-1/HIV-2抗体、梅毒抗体和丙肝抗体的检测

    Institute of Scientific and Technical Information of China (English)

    姜大娥

    2012-01-01

    目的:比较ELISA法和化学发光法对血清中HIV - 1/HIV -2抗体、梅毒抗体和丙肝抗体的检测.方法:用ELISA法与化学发光法对病人血清和标准物质进行以上三种抗体的检测,并对结果进行分析.结果:(1)化学发光法测定值较低的阳性标本,ELISA法测定有可能为阴性.(2)用化学发光法检测病人血清中HIV -1/HIV -2抗体为阴性标本的测定数据有一定程度的波动.(3)同一公司生产的以上三种抗体的标准物质,用ELISA均为阳性,可是用化学发光法仅丙肝抗体检测为阳性,而其它两种均为阴性.结论:基本证实了化学发光法比ELISA法敏感性高.%Objective:To compare the ELJSA and chemiluminescence assay in test of serum HIV - 1/HIV -2 antibody, anti -hepatitis C antibodies and syphilis antibody. Methods; The above three antibodies were detected in serum of patients and standard substance by ELISA and chemiluminescence assay. Then the results were analyzed. Results; 1. Samples, which were found positive with low measured value by chemiluminescence assay, may be found negative by ELJSA. 2. Samples, which were detected by chemiluminescence assay with serum HIV - 1/HIV -2 antibodies negative may exist a certain rise and fall. 3. The standard substance of the above three antibodies from the same company was positive by ELISA, while with chemiluminescence assay, hepatitis C antibody was positive but the other were both negative. Conclusion: The sensitivity of chemiluminescence assay was higher than that of ELISA.

  8. 基于纳米TiO2的化学发光法检测核黄素%Determination of Riboflavin by Chemiluminescence Method Based on TiO2 Nanoparticles

    Institute of Scientific and Technical Information of China (English)

    申丽华; 吴波; 于春侠; 魏海霞

    2013-01-01

    Chemiluminescence emission was first observed in the interaction of TiO2 nanoparticles with NaOH solution. In the presence of surfactant of CTAB, the riboflavin can enhance the chemilu minescence intsnisty of TiO2 Nanoparticle - NaOH. Based on this, a new chemiluminescence system of TiO2 NPs - NaOH was fabricated, and a novel chemiluminescence method for determination of riboflavin was developed. Various factors affecting the CL emission intensity of the system were investigated. Under the optimum conditions, the linear range for the determination of riboflavin was in the range of 5. 0 ×10 -6 - 3. 5 x 10-4 g/mL, with a detection limit of 3. 0 × 10 -6 g/mL. The relative standard deviation(n = 11) for the determination of 2. 5 ×10 -5 g/mL riboflavin sodium was 2. 9% . The method was applied in the determination of riboflavin preparation of tablets, and the results were in good agreement with those obtained by the pharmacopoeia method. The mechanism of the CL reaction was also discussed briefly.%纳米TiO2与NaOH溶液作用能产生化学发光辐射,在表面活性剂十六烷基三甲基溴化铵(CTAB)的存在下,核黄素的加入能增强纳米TiO2-NaOH溶液的化学发光强度.基于此,构建了纳米TiO2-NaOH溶液化学发光新体系,建立了纳米TiO2-NaOH-核黄素体系检测核黄素的化学发光新方法.在优化实验条件下,核黄素质量浓度在5.0×10-6~ 3.5×10-4 g/mL范围内与化学发光强度呈良好的线性关系,检出限为3.0×10-6 g/mL,对2.5×10-5 g/mL的核黄素进行11次平行测定,相对标准偏差为2.9%.该方法用于维生素B2片剂的测定,其结果与药典方法测得一致.该文同时对化学发光反应的机理进行了初步探讨.

  9. Detection of Matrix Metalloproteinase-11in Menstrual Blood by Enhanced Chemiluminescence Method%增强化学发光法检测基质金属蛋白酶-11鉴定月经血

    Institute of Scientific and Technical Information of China (English)

    章雅清; 陆惠玲; 姚亚楠

    2012-01-01

    目的 探讨增强化学发光法检测人基质金属蛋白酶-11(matrix metalloproteinase-11,MMP- 11)鉴定月经血的法医学应用价值.方法 运用增强化学发光法检测月经血痕、阴道拭子、外周血痕、唾液斑、尿液斑和精液斑中的MMP-11,考察MMP-11的组织特异性、稳定性及检测方法的灵敏度.结果 月经血痕中MMP-11蛋白的检测阳性率为89.47%,阴道拭子、外周血痕、唾液斑、尿液斑和精液斑中均未检出MMP-11.当上样量为25 μL时,蛋白质量浓度为1.329 μg/μL,可检出MMP-11.4℃放置20个月的MMP-11阳性的月经血痕,检测阳性率为89.58%.结论 用增强化学发光法检测MMP- 11蛋白的方法灵敏度高、特异性好,能用于月经血与外周血、阴道液等常见斑痕的鉴别.%Objective To explore the forensic application value of detection of matrix metalloproteinase-11 (MMP-11) in menstrual blood by enhanced chemiluminescence method. Methods Menstrual blood, vaginal swab, peripheral blood, saliva stain, urine stain and semen stain were collected to detect whether or not there were MMP-11 using enhanced chemiluminescence method. The specificity and reliability of the MMP-11 assay along with its sensitivity were evaluated. Results The positive detection rate of MMP-11 in menstrual blood was 89.47%, whereas no MMP-11 was found in vaginal swab, peripheral blood, saliva stain, urine stain and semen stain. When 25 μL sample was added, the mass concentration of protein was 1.329 μg/μL, then MMP-11 could be detected. A positive detection rate of 89.58% was observed in MMP-11 positive menstrual blood samples after stored at 4℃ for 20 months. Conclusion Enhanced chemiluminescence method is sensitive and specific for detecting MMP-11, and can be applied to distinguish menstrual blood from common stain such as peripheral blood, vaginal fluid.

  10. Evaluation of luminol chemiluminescence based on simultaneous introducing of coumarin derivatives as green fluorophores and chitosan-induced Au/Ag alloy nanoparticle as catalyst for the sensitive determination of glucose.

    Science.gov (United States)

    Chaichi, M J; Alijanpour, S O; Asghari, S; Shadlou, S

    2015-03-01

    We report herein the development of a novel chemiluminescence system based on simultaneous introducing of synthetic coumarin derivatives and chitosan-induced Au/Ag alloy NPs on the luminol CL system and suggest how it may be useful for determination of glucose. Chitosan-induced Au/Ag nanoalloys in the coumarin derivatives intensified-luminol CL system, in addition to catalyze CL reaction can make a change in the process of coumarin derivatives effect as fluorophore on the luminol CL system. This phenomenon is caused by interaction between active functional groups of coumarin derivatives and chitosan. The interaction strength depends on the coumarin derivatives' structure and their substituents. Considering the inevitable trend luminol radical and superoxide anion radical to absorption on the surface of the embedded Au/Ag nanoalloy in the chitosan matrix, it can be concluded that chitosan acts as a platform for all reagents involved in the CL reaction including coumarin derivatives, Au/Ag nanoalloy and luminol, and electron-transfer taking place on it; Placing all chemiluminescent reagents together on the chitosan network can lead to a powerful CL due to increasing rigidity of CL system. The most efficient coumarin derivative on the Au/Ag nanoalloy-fluorophore-luminol-H2O2 CL system, in relation to interaction capability with chitosan' functional groups, was selected and the CL condition in presence of it was optimized. Whereas the glucose oxidase-mediated oxidation of glucose yields gluconic acid and H2O2, under optimum condition the most efficient CL system was applied to detection of glucose due to enzymatically production of hydrogen peroxide. The linear response range of 1.5 × 10(-6)-5.0 × 10(-3) M and the detection limit (defined as the concentration that could be detected at the signal-to-noise ratio of 3) of 7.5 × 10(-7) M was found for the glucose standards. Also, the developed method was successfully applied to determination of glucose in

  11. 固相萃取—化学发光法测定植物中的吲哚乙酸%Solid-phase extraction-flow injection chemiluminescence determination of indoleacetic acid in Arabidopsis

    Institute of Scientific and Technical Information of China (English)

    米娟; 周敏; 丁兰; 李玉杰; 刘彩云; 马永钧; 陈慧

    2011-01-01

    植物拟南芥中吲哚乙酸用甲醇超声提取,后经固相萃取小柱净化用化学发光法测定.在多聚磷酸(PPA)介质中,吲哚乙酸对高良姜素-高锰酸钾体系的发光有很强的增敏作用,据此建立了固相萃取-流动注射化学发光测定吲哚乙酸的新方法.在优化条件下,相对化学发光的对数值与吲哚乙酸浓度的对数值在8.0 ×10-9~1.0×10 -7g/mL和1.0×10-7~1.0×10-6g/mL范围内呈良好的线性关系,检出限为2.8×10 -9g/mL.对1.0×10-7g/mL的吲哚乙酸进行10次平行测定,相对标准偏差为2.6%.利用本方法测定对映-贝壳杉烷二萜化合物Leukamenin E处理前后拟南芥中吲哚乙酸含量的变化,回收率在90.9%~100.9%之间.%Indoleacetic acid (IAA) in Arabidopsis is extracted with methanol in an ultrasonic bath,and purified by solid phase extraction (SPE) followed by flow injection-chemiluminescent (FI-CL) detection. The method is based on the enhancement of IAA on the chemiluminescence of galangin-potassium permanganate-poly phosphoric acid system. Under the optimum conditions,the obtained linear range is 8. 0 X 10-9 ~ 1. 0 X 10-7 g/mL and 1. 0 x 10-7 ~ 1. 0 x 10-6 g/mL,respectively,with a detection limit of 2. 8 x 10-9 g/mL. The relative standard deviation (RSD) for the determination of 1.0 x 10-7 g/mL IAA is 2. 6% ( n = 10). The proposed method has been used for the determination of IAA in Arabidopsis with/ and Leukamenin E ( an ent-kaurane diterpenoid) and the recoveries are in the range from 90. 3% to 101. 0%.

  12. Hypericin from St. John’s Wort (hypericum perforatum) as a novel natural fluorophore for chemiluminescence reaction of bis (2,4,6-trichlorophenyl) oxalate–H2O2–imidazole and quenching effect of some natural lipophilic hydrogen peroxide scavengers

    International Nuclear Information System (INIS)

    Hypericin (HYP) molecule is a natural photoactive pigment, which plays a role as an effective photoreceptor in some plants of the Hypericum species (the most common of which is Saint John’s Wort) and some insect species. The present work deals with the first attempt to the study of peroxyoxalate chemiluminescence (POCL) system in the presense of HYP as a natural fluorophore. Reaction of bis (2,4,6-trichlorophenyl) oxalate(TCPO)–H2O2–imidazole can transfer energy to a HYP via formation of dioxetane through the chemically initiated electron exchange luminescence (CIEEL) mechanism and can emits a very intense red light. The effects of HYP, hydrogen peroxide, TCPO and imidazole concentrations on kinetic chemiluminescence parameters were also studied. These parameters including rise and fall rate constant for the chemiluminescence burst, theoretical and experimental maximum intensity, theoretical and experimental time to reach maximum intensity and total light yield emission were evaluated by using a pooled intermediate model for a non-linear least-squares curve fitting program, KINFIT. Moreover, quenching effect of two lipophilic natural antioxidant, Quercetin and β-carotene on it system was also investigated. The measurable concentration range of 7×10−6 M to 7.5×10−5 M of antioxidants were evaluated from the proper Stern–Volmer plots with satisfactory RSD% and corresponding detection limits of 2.2×10−6 and 3.7×10−6 for β-carotene and quercetin respectively. - Highlights: ► Red fluorophores may therefore chemiluminescence more intensely than other commonly chemiluminophores and emits light in longer wavelengths. ► Hypericin from St. John’s wort (hypericum perforatum) as natural red fluorophore for peroxyoxalate chemiluminescence was introduced. ► Quenching effect of two antioxidant, quercetin and β-carotene on it system was also investigated. ► The non linear least-squares curve fitting program KINFIT was applied to study of CL kinetic

  13. Hypericin from St. John's Wort (hypericum perforatum) as a novel natural fluorophore for chemiluminescence reaction of bis (2,4,6-trichlorophenyl) oxalate-H{sub 2}O{sub 2}-imidazole and quenching effect of some natural lipophilic hydrogen peroxide scavengers

    Energy Technology Data Exchange (ETDEWEB)

    Kazemi, Sayed Yahya [Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari (Iran, Islamic Republic of); Abedirad, Seyed Mohammad, E-mail: mabedirad@yahoo.com [Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari (Iran, Islamic Republic of); Zali, Seyed Hassan; Amiri, Mohadeseh [Department of Range and Watershed Management, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari (Iran, Islamic Republic of)

    2012-05-15

    Hypericin (HYP) molecule is a natural photoactive pigment, which plays a role as an effective photoreceptor in some plants of the Hypericum species (the most common of which is Saint John's Wort) and some insect species. The present work deals with the first attempt to the study of peroxyoxalate chemiluminescence (POCL) system in the presense of HYP as a natural fluorophore. Reaction of bis (2,4,6-trichlorophenyl) oxalate(TCPO)-H{sub 2}O{sub 2}-imidazole can transfer energy to a HYP via formation of dioxetane through the chemically initiated electron exchange luminescence (CIEEL) mechanism and can emits a very intense red light. The effects of HYP, hydrogen peroxide, TCPO and imidazole concentrations on kinetic chemiluminescence parameters were also studied. These parameters including rise and fall rate constant for the chemiluminescence burst, theoretical and experimental maximum intensity, theoretical and experimental time to reach maximum intensity and total light yield emission were evaluated by using a pooled intermediate model for a non-linear least-squares curve fitting program, KINFIT. Moreover, quenching effect of two lipophilic natural antioxidant, Quercetin and {beta}-carotene on it system was also investigated. The measurable concentration range of 7 Multiplication-Sign 10{sup -6} M to 7.5 Multiplication-Sign 10{sup -5} M of antioxidants were evaluated from the proper Stern-Volmer plots with satisfactory RSD% and corresponding detection limits of 2.2 Multiplication-Sign 10{sup -6} and 3.7 Multiplication-Sign 10{sup -6} for {beta}-carotene and quercetin respectively. - Highlights: Black-Right-Pointing-Pointer Red fluorophores may therefore chemiluminescence more intensely than other commonly chemiluminophores and emits light in longer wavelengths. Black-Right-Pointing-Pointer Hypericin from St. John's wort (hypericum perforatum) as natural red fluorophore for peroxyoxalate chemiluminescence was introduced. Black-Right-Pointing-Pointer Quenching

  14. Chitosan-induced Au/Ag nanoalloy dispersed in IL and application in fabricating an ultrasensitive glucose biosensor based on luminol-H₂O₂-Cu²⁺/IL chemiluminescence system.

    Science.gov (United States)

    Chaichi, M J; Alijanpour, S O

    2014-11-01

    A novel glucose biosensor based on the chemiluminescence (CL) detection of enzymatically generated hydrogen peroxide (H₂O₂) was constructed by one covalent immobilization of glucose oxidase (GOD) in glutaraldehyde-functionalized glass cell. In following, chitosan-induced Au/Ag nanoparticles dispersed in ion liquid (IL) were synthesised and immobilized on it. Herein, chitosan molecules acted as both the reducing and stabilizing agent for the preparation of NPs and also, as a coupling agent GOD and Au/Ag alloy NPs. In addition to catalyze luminol CL reaction, these NPs offered excellent catalytic activity toward hydrogen peroxide generation in enzymatic reaction between GOD and glucose. The used IL in fabrication of biosensor increased its stability. Also, IL alongside Cu(2+) accelerated enzymatic and CL reaction kinetic, and decreased luminol CL reaction optimum pH to 7.5 which would enable sensitive and precision determination of glucose. Under optimum condition, linear response range of glucose was found to be 1.0 × 10(-6)-7.5 × 10(-3)M, and detection limit was 4.0 × 10(-7)M. The CL biosensor exhibited good storage stability, i.e., 90% of its initial response was retained after 2 months storage at pH 7.0. The present CL biosensor has been applied satisfactory to analysis of glucose in real serum and urine samples. PMID:25086323

  15. 催化裂化油浆中硫化物气相色谱分析%Determination of Sulfur Compounds in Catalytic Cracking Slurry by Gas Chromatography With Sulfur Chemiluminescence Detection

    Institute of Scientific and Technical Information of China (English)

    何俊辉; 贾广信; 黎爱群; 薛晓军

    2014-01-01

    采用气相色谱配硫化学发光检测器(SCD)结合高温模拟蒸馏ASTM D7169-05方法对巴陵石化催化裂化油浆中含硫化合物进行了分析鉴定,结果表明:油浆的馏程在253℃到690℃左右范围内,硫化物类型主要是二苯并噻吩类和萘并噻吩类化合物;定量分析结果表明,萘并噻吩类化合物占油浆中总硫化物含量的70%以上。%The catalytic cracking slurry was analyzed by gas chromatography with sulfur chemiluminescence detection (GC-SCD) and SimDis ASTM D7169-05 method. The results show that distillation range of the slurry is from 253 ℃to 690 ℃,and main sulfide types are dibenzothiophenes and naphthothiophenes. Quantitative analysis results show that naphthothiophene compounds account for more than 70%of the total sulfur content in the slurry.

  16. 化学发光生物传感器检测食品中生物胺总量%Detection of total biogenic amines in foods by chemiluminescence biosensor

    Institute of Scientific and Technical Information of China (English)

    唐晗; 王晓朋; 吴中波; 万德慧; 刘晓宇

    2014-01-01

    目的:建立快速地有效检测食品中生物胺总量的分析方法。方法用固定化的二胺氧化酶制成酶柱作为生物传感器的识别元件,将微流控芯片与化学发光仪结合作为检测元件,以鲁米诺-铁氰化钾作为化学发光体系,通过流动分析法来检测食品中生物胺的总量。结果当腐胺、组胺、酪胺浓度分别在3~500、2~100、3~200μmol/L时,线性回归方程分别为Y=17.448X+408.93、Y=88.329X+997.13、Y=45.762X+1728.2,相关系数分别为0.9966、0.9937、0.9907,检出限分别为0.7、0.5、0.5μmol/L。另外,本研究对传感器的性能进行了评价。结果显示,在最佳条件下对含有腐胺、组胺、酪胺的溶液分别测定7次,其RSD均小于8%,精密度较好;以腐胺为底物,连续通入鲁米诺和铁氰化钾溶液7次, RSD=1.66%,表现出良好的稳定性;将固定化酶保存在磷酸盐缓冲液中,每10 d测定一次酶活,持续70 d。2个月内固定化二胺氧化酶酶活仅降低了15%,保存时间较长;将传感器用于检测猪肉、鲫鱼和葡萄酒中的生物胺总量,样品中三种胺的添加回收率均在90%~94%之间,回收率较高,适合检测总生物胺。结论此传感器的综合性能良好,适用于食品中生物胺总量的快速检测。%Objective To establish a method for the analysis of the biogenic amines content in foods. Methods A novel micro-fluidic chip-chemiluminescence-enzyme biosensor was designed and assembled to determine the total biogenic amines content in foods. Commercial diamine oxidase was used as biological recognition element of the sensor, the micro-fluidic chip with chemiluminescence analyzer was used as a sensing element, and luminol-ferricyanide was used in chemiluminescence system. Results When the concentration of putrescine, histamine, and tyramine were 3~500, 2~100, 3~200μmol/L, the linear regression equation were Y=17.448X+408.93, Y=88.329X+997.13, Y=45.762X+1728.2, the correlation coefficient

  17. Determination of propoxur in environmental samples by automated solid-phase extraction followed by flow-injection analysis with tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection

    Energy Technology Data Exchange (ETDEWEB)

    Perez-Ruiz, Tomas [Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia (Spain)]. E-mail: tpr@um.es; Martinez-Lozano, Carmen [Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia (Spain); Garcia, Maria Dolores [Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia (Spain)

    2007-02-19

    A sensitive method for the analysis of propoxur in environmental samples has been developed. It involves an automated solid-phase extraction (SPE) procedure using a Gilson Aspec XLi and flow-injection analysis (FI) with chemiluminescence (CL) detection. The FI-CL system relies on the photolysis of propoxur by irradiation using a low-pressure mercury lamp (main spectral line 254 nm). The resultant methylamine is subsequently detected by CL using tris(2,2'-bipyridyl)ruthenium(III), which is on-line generated by photo-oxidation of the ruthenium(II) complex in the presence of peroxydisulfate. The linear concentration range of application was 0.05-5 {mu}g mL{sup -1} of propoxur, with a detection limit of 5 ng mL{sup -1}. The repeatability was 0.82% expressed as relative standard deviation (n = 10) and the reproducibility, studied on 5 consecutive days, was 2.1%. The sample throughput was 160 injection per hour. Propoxur residues below ng mL{sup -1} levels could be determined in environmental water samples when an SPE preconcentration device was coupled on-line with the FI system. This SPE-FI-CL arrangement provides a detection limit as low as 5 ng L{sup -1} using only 500 mL of sample. In the analysis of fruits and vegetables, the detection limit was about 10 {mu}g kg{sup -1}.

  18. Determination of propoxur in environmental samples by automated solid-phase extraction followed by flow-injection analysis with tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection.

    Science.gov (United States)

    Pérez-Ruiz, Tomás; Martínez-Lozano, Carmen; García, María Dolores

    2007-02-19

    A sensitive method for the analysis of propoxur in environmental samples has been developed. It involves an automated solid-phase extraction (SPE) procedure using a Gilson Aspec XLi and flow-injection analysis (FI) with chemiluminescence (CL) detection. The FI-CL system relies on the photolysis of propoxur by irradiation using a low-pressure mercury lamp (main spectral line 254 nm). The resultant methylamine is subsequently detected by CL using tris(2,2'-bipyridyl)ruthenium(III), which is on-line generated by photo-oxidation of the ruthenium(II) complex in the presence of peroxydisulfate. The linear concentration range of application was 0.05-5 microg mL(-1) of propoxur, with a detection limit of 5 ng mL(-1). The repeatability was 0.82% expressed as relative standard deviation (n=10) and the reproducibility, studied on 5 consecutive days, was 2.1%. The sample throughput was 160 injection per hour. Propoxur residues below ng mL(-1) levels could be determined in environmental water samples when an SPE preconcentration device was coupled on-line with the FI system. This SPE-FI-CL arrangement provides a detection limit as low as 5 ng L(-1) using only 500 mL of sample. In the analysis of fruits and vegetables, the detection limit was about 10 microg kg(-1).

  19. Sensitized chemiluminescence of 2-phenyl-4,5-di(2-furyl)-1H-imidazole/K₃Fe(CN)₆/propyl gallate system combining with solid-phase extraction for the determination of propyl gallate in edible oil.

    Science.gov (United States)

    Kang, Jing; Han, Lu; Chen, Zhonglin; Shen, Jimin; Nan, Jun; Zhang, Yihua

    2014-09-15

    In this paper, a novel chemiluminescence (CL) method has been developed for the determination of propyl gallate (PG). The proposed method was based on the enhancing effect of PG on the CL signal of 2-phenyl-4,5-di(2-furyl)-1H-imidazole (PDFI) and K3Fe(CN)6 reaction in an alkaline solution. Under the optimum conditions, the enhanced CL intensity was linearly related to the concentration of PG. The linear range of the calibration curve was 0.05-8 μg/mL, and the corresponding detection limit (3σ) was 0.036 μg/mL. The relative standard deviation for determining 1.0 μg/mL PG was 2.8% (n=11). The proposed method has been successfully applied to the determination of PG in edible oil. The edible oil samples were prepared by the solid-phase extraction (SPE) with a C18 column served as the stationary phase. Furthermore, the possible CL mechanism was also discussed briefly based on the photoluminescence (PL) and CL spectra.

  20. Detection of human serum free thyroxine by non-equilibrium competitive chemiluminesce immunoassay using FITC system%应用FITC系统建立非均衡竞争游离甲状腺素化学发光法

    Institute of Scientific and Technical Information of China (English)

    刘萍; 孔海霞; 张影; 田云霞; 宋启超; 涂值光

    2012-01-01

    [目的]制备抗人甲状腺素多克隆抗体并应用异硫氰酸荧光素(fluorescein isothiocyanate,FITC)系统建立非均衡竞争FT4化学发光法.[方法]以T4-牛血清白蛋白(bovin serum albumin,BSA)为抗原免疫新西兰兔,制备抗人T4多克隆抗体,亲和层析法吸附掉与3-碘-L-甲状腺原氨酸(3-iodo-L-tyrosine)、3,5-二碘-L-甲状腺原氨酸(3,5-diiodo-L-tyrosine)、D-三碘甲状腺原氨酸(D-T3)、L-三碘甲状腺原氨酸(L-T3)、反三碘甲状腺原氨酸(rT3)有交叉反应多抗,纯化特异性的抗人T4多克隆抗体并用FITC标记T4类似物,用抗FITC抗体包被微孔板.反应时,将FT4校准品或样品、FITC-T4类似物、T4抗体-HRP依次加入包被有抗FTTC抗体的化学发光板中,FITC -T4类似物与FT4校准品或样品,非均衡竞争结合T4抗体-HRP,从而在微孔板上形成免疫反应复合物T4类似物-T4抗体-HRP,通过酶促化学发光反应,就可以显示出与样品FT4浓度负相关的相对光单位(relative light unit,RLU),建立FT4化学发光检测方法.[结果]抗体经酶联免疫吸附实验证明对T4具有高度特异性,与3-iodo-L-tyrosine、3,5-diiodo-L-tyrosine、L-T3、D-T3、rT3无交叉反应.该方法建立的试剂盒标准曲线的线性相关系数不小于0.9900;分析灵敏度为0.9 pmol/L;线性检测范围0.9~130 pmol/L;精密度测试批内变异系数CV< 10%;与进口发光免疫分析系统做线性相关比较,线性回归系数为1.0852,相关系数为0.9673,临床符合率良好;本方法建立的试剂盒经加速稳定性试验37℃放置7d与2~8℃放置7d无异.[结论]综上所述,应用FITC系统建立的非均衡竞争FT4化学发光免疫分析灵敏度高、特异性好适于临床诊断应用.%[Objective]To prepare polyclonal antibodies(mAb) against human thyroxine and to establish a new method of non-equilibrium competitive chemiluminescence immunoassay (CLIA) with fluorescein isothiocyanate (FITC) for serum free thyroxine

  1. Methodological evaluation of chemiluminescence microparticle immunoassay for determining HE4%化学发光微粒子免疫法检测人附睾蛋白方法学性能评价

    Institute of Scientific and Technical Information of China (English)

    郑桂喜; 王传新; 刘延红; 梁永媛

    2012-01-01

    目的 对Abbott Architect i2000SR检测系统应用化学发光微粒子免疫技术定量测定人附睾蛋白(HE4)的分析性能进行评价.方法 参考美国临床实验室标准化协会( NCCLS)文件,制定定量检测方法的方法学评价方案,通过Abbott Architect i2000sR检测系统测定HE4的批内精密度、批间精密度、线性范围、携带污染率和参考区间.结果 低、高值混合血清批内精密度CV分别为1.80%、2.34%;批间精密度CV分别为2.03%、2.70%;线性范围为19.8 ~1 573.5 pmol/L;携带污染率为-0.02%;参考区间为0.0~ 140.0 pmol/L.结论 Abbott Architect i2000SR检测系统测定HE4的精密度好、线性范围宽、携带污染率低,参考区间与厂家提供的一致,检测性能可满足临床要求.%To evaluate the performance methodology of Chemiluminescence microparticle immunoassay (CMIA) for determining HE4 using Abbott Architect i2000SR Chemiluminescence analyzer. Methods According to a method by NCCLS recommended, we designed the evaluation program of quantitative detection methods. The within-run precision、 between-run precision Jinear range,carry-over and biological reference interval of HE4 were determined. Results The coefficient of variation( CV) of within-run precision of low and high value pooled serum were respectively 1. 80% and 2. 34% . The CV of between-run precision were respectively 2. 03% and 2. 70% . The linear range was from 19.8 pmol/L to 1 573.5 pmol/L. The 1 carry-over was -0.02%. The biological reference interval was consistent with manufactures which is from 0. 0 pmol/L to 140. 0 pmol/L. Conclusion The within-run precision, between-run precision, linear range and carry-over of Abbott Architect i2000SR for determining HE4 meet the requirements of clinical use. The biological reference interval was consistent with that provided by the manufacturer.

  2. 化学发光酶免疫法测牛奶中3种喹诺酮类药物%Determination of QNs Residues in Milk by Enhanced Chemiluminescent Enzyme Immunoassay

    Institute of Scientific and Technical Information of China (English)

    李源珍; 生威; 刘恩梅; 韩静; 秦沛; 王硕

    2013-01-01

    A direct competitive enhanced chemiluminescent enzyme immunoassay(dc-CLEIA) for determination of ofloxacin (OFL), marbofloxacin (MAR), flerofloxacin (FLE) residues in milk was developed. The detection limit of assay were 0.01, 0.03, 0.04 ng/mL respectively. The spiked milk samples were extracted with trichloroacetic acid and analyzed by dc-CLEIA. The average recoveries at five spiked levels of 200, 100, 50, 20, 10 ng/mL were between 75.4%and 94.1%. There was a good correlation between data obtained using the dc-CLEIA and HPLC, indicating the good performance of this dc-CLEIA. Therefore, the proposed method was simple, accurateand and suitable for the rapid determination of ofloxacin, marbofloxacin and flerofloxacin in milk samples.%建立可同时检测牛奶中氧氟沙星(OFL)、麻保沙星(MAR)、氟罗沙星(FLE)残留的直接竞争化学发光酶免疫法(dc-CLEIA),方法的检出限分别为0.01、0.03、0.04 ng/mL。牛奶中的氧氟沙星、麻保沙星、氟罗沙星用7.5%的三氯乙酸提取,测定200、100、50、20、10 ng/mL 5个添加水平的回收率,平均回收率在75.4%~94.1%之间。HPLC与dc-CLEIA测定的结果有很好的相关性,说明所建立的dc-CLEIA可用于实际样品的检测,结果准确可靠。

  3. Quantitative analysis of nitrogen containing compounds in microalgae based bio-oils using comprehensive two-dimensional gas-chromatography coupled to nitrogen chemiluminescence detector and time of flight mass spectrometer.

    Science.gov (United States)

    Toraman, Hilal E; Franz, Kristina; Ronsse, Frederik; Van Geem, Kevin M; Marin, Guy B

    2016-08-19

    Insight in the composition of the algae derived bio-oils is crucial for the development of efficient conversion processes and better upgrading strategies for microalgae. Comprehensive two-dimensional gas chromatography (GC×GC) coupled to nitrogen chemiluminescence detector (NCD) and time-of-flight mass spectrometer (TOF-MS) allows to obtain the detailed quantitative composition of the nitrogen containing compounds in the aqueous and the organic fraction of fast pyrolysis bio-oils from microalgae. Normal phase (apolar×mid-polar) and reverse phase column (polar×apolar) combination are investigated to optimize the separation of the detected nitrogen containing compounds. The reverse phase column combination gives the most detailed information in terms of the nitrogen containing compounds. The combined information from the GC×GC-TOF-MS (qualitative) and GC×GC-NCD (quantitative) with the use of a well-chosen internal standard, i.e. caprolactam, enables the identification and quantification of nitrogen containing compounds belonging to 13 different classes: amines, imidazoles, amides, imides, nitriles, pyrazines, pyridines, indoles, pyrazoles, pyrimidines, quinolines, pyrimidinediones and other nitrogen containing compounds which were not assigned to a specific class. The aqueous fraction mostly consists of amines (4.0wt%) and imidazoles (2.8wt%) corresponding to approximately 80wt% of the total identified nitrogen containing compounds. On the other hand, the organic fraction shows a more diverse distribution of nitrogen containing compounds with the majority of the compounds quantified as amides (3.0wt%), indoles (2.0wt%), amines (1.7wt%) and imides (1.3wt%) corresponding to approximately 65wt% of the total identified nitrogen containing compounds. PMID:27432785

  4. 偶合反应流动注射化学发光法测定扑热息痛%Flow injection chemiluminescence determination of paracetamol with chromium (VI)-H202-luminol system

    Institute of Scientific and Technical Information of China (English)

    王书民; 樊雪梅; 苏智魁; 陈凤英; 刘萍

    2011-01-01

    As chromium(Ⅵ) can be reduced by paracetamol to chromium(Ⅲ) which enhance the CL intensity of luminol-H2O2system, a new chemiluminescent method for the determination of paracetamol was developed. The relative CL intensity was linearly related to the concentration of paracetamol in the range of 4. 0 × 10-9 ~4. 0 ×10-5mol/L with a detection limit of 1.0 × 10-9mol/L. The relative standard deviation( n = 8) for 4. 0 × 10-7mol/L paracetamol was 2. 7%. The method has been applied to the determination of paracetamol in the tablets.%基于扑热息痛还原铬(Ⅵ)和铬(Ⅲ)催化鲁米诺-过氧化氢的化学发光,建立了氧化还原偶合反应流动注射化学发光法测定扑热息痛的新方法.方法线性范围为4.0×10-9~4.0×10-5mol/L,检出限为1.0×10-9 mol/L.对4.0×10-7 mol/L扑热息痛平行测定8次,其标准偏差为2.7%.已将该方法用于片剂中扑热息痛含量测定.

  5. 流动注射-Luminol/Tween20化学发光体系测定邻苯二酚%Determination of catechol with flow injection-luminol/Tween 20 chemiluminescence system

    Institute of Scientific and Technical Information of China (English)

    谢建新; 伍贤学

    2013-01-01

    基于邻苯二酚对luminol-Tween 20化学发光体系的强烈的抑制作用,建立了一种简单、灵敏的luminol-Tween 20化学发光体系测定邻苯二酚的新方法.在最佳实验条件下,方法的检出限为5.0 ×l0-10mo1/L,线性范围为1.0 × 10-9mol/L~1.0×10-2mol/L,对5.0×10-8mol/L邻苯二酚进行平行测定11次,其RSD为4.1%.该方法可应用于自来水样中邻苯二酚的测定.%A simple and sensitive flow injection-chemiluminescence (CL) system of luminol-Tween 20 for the direct determination of catechol was developed.Proposed method was based on the strongly inhibition effect of catechol on the CL intensity of luminol-Tween 20 system.Under the optimial conditions,catechol was determined.The detection limit of the method is 5.0 × 10-10mol/L for catechol,the linear range for catechol is 1.0 × 10-9mol/L ~ 1.0 × 10-7mol/L.The relative standard deviation is 4.1% for 5.0 × 10-8mol/L catechol in 11 repeated measurements.The method was successfully applied to the detection of catechol in tap water samples.

  6. Determination of Paracetamol in Pharmaceuticals by Fe3+ -H2O2 -Dichlorofluorescein Chemiluminescence System%Fe3+-H2O2-二氯荧光素化学发光体系测定药物中的扑热息痛

    Institute of Scientific and Technical Information of China (English)

    樊雪梅; 王书民; 尹治民; 苏智魁; 陈凤英; 王宪生; 崔凤灵

    2012-01-01

    A novel flow-injection chemiluminescence method was developed for the determination of paracetamol, which was based on the chemiluminescence reaction between paracetamol and hydroxy free radical produced by Fe3+ and H2O2 solution in the presence of dichlorofluorescein as energy transfer in the chemiluminescence system. Various factors influencing the chemiluminescence system were investigated and the possible mechanism was also discussed. Under the optimum conditions, the linear range for the determination of paracetamol was 8. 0×10 -8 - 5. 0 ×10-5 mol/L with a detection limit(S/N=3) of 5.0 ×10-9 mol/L, the relative standard deviation ( RSD, n =9) was 2. 2% for 3. 5×10-6 mol/L paracetamol solution. The method was successfully applied in the determination of paracetamol in pharmaceutical sample.%酸性介质中,Fe3+催化H2O2分解生成羟基自由基,进而氧化扑热息痛产生微弱的化学发光,二氯荧光素对该发光强度有较强的增敏作用.研究了影响化学发光强度的各种因素,并探讨了其可能的发光机理.在最佳化学发光条件下,其化学发光强度与扑热息痛的浓度在8.0×10-8~5.0×10-5 mol/L范围内呈良好的线性关系,检出限为5.0×10-9 mol/L,对3.5 ×10-6 mol/L的扑热息痛平行测定9次,其相对标准偏差为2.2%.该法用于片剂中扑热息痛含量的测定,结果满意.

  7. Chemiluminescence analysis of amitriptyline hydrochloride with a nanometer microreactor%流动注射纳米微反应器化学发光法测定盐酸阿米替林

    Institute of Scientific and Technical Information of China (English)

    朱乾华

    2011-01-01

    In acidic condition, the nitrogen in amitriptyline hydrochloride was easily protonated and formed ion associated-complex with negative ion AuCl4-, the ion associated-complex was extracted and carried by dichloromethane, when the ion associated-complex entered a reversed micellar nanometer microreactor of cetyltrimethylammonium chlorine containing luminol, the dissociated AuCl4- reacted with luminol and produced an analytical chemiluminescence signal. Under the optimum conditions, the linear range is from 0.001 to 15 μg/mL and the limit of detection is 0.03 ng/mL. The relative standard deviation (RSD) is 2.4% for 1.0 μg/mL amitriptyline hydrochloride (n = 11 ). The method has been applied to the determination of the studied drug in tablets and biological fluids successfully.%在酸性条件下,盐酸阿米替林分子中氮原子被质子化后与阴离子AuCl4-形成离子缔合物,该缔合物被二氯甲烷带入鲁米诺的氯化十六烷基三甲基铵反胶束纳米微反应器中,离解出来的AuCl4-立即与鲁米诺产生化学发光.在一定浓度范围内,发光强度与盐酸阿米替林的含量成线性关系,从而间接测定盐酸阿米替林的含量.在优化的实验条件下,线性范围为0.001~15 μg/mL,检出限(38)为0.03 ng/mL,对浓度为1.0μg/mL的盐酸阿米替林进行11次平行测定,RSD为2.4%.该法已用于片荆和生物体液中盐酸阿米替林的测定.

  8. Development of Chemiluminescence Enzyme Immunoassay for the Determination of Ochratoxin A in Cereal%谷物中赭曲霉毒素A化学发光酶免疫分析法的建立

    Institute of Scientific and Technical Information of China (English)

    刘星; 许杨; 何庆华

    2011-01-01

    建立了间接竞争化学发光酶免疫法检测赭曲霉毒素A(ochratoxin A,OTA),该方法IC50为112 pg/mL,检出限是2.47 pg/mL,平均批内和批间变异系数分别为7.03%和14.7%.在大米和小麦样本中添加浓度1.5~6 μg/kg的OTA标品,平均回收率在66.97%~97.96%之间,与其他常见真菌毒素未见交叉反应.将该方法应用于30份谷物样本(包括20份大米样本和10份小麦样本)中OTA的检测,检测结果与商品化ELISA试剂盒的相关系数R2= 0.942 4.该方法简单、灵敏、快速、准确适用于谷物中OTA的检测.%A sensitive indirect competitive chemiluminescence enzyme immunoassay (CLEIA)for OTA was developed. The concentration of OTA causing 50% inhibition of binding enzyme marker (IC50) was 112 pg/mL and the detection limit was 2.47 pg/mL, the mean coefficients of variation of within groups and between groups were 7.03,14.7% respectively. The recoveries from rice and wheat samples spiked with 1.5 ~ 6 Ig/kg of OTA varied between 66.97% and 97.96% ,and the OTA antibody showed no cross -reactivity to the else common mycotoxins. OTA in 30 cereal samples were screened by the CLEIA, including 20 rice samples and 10 wheat samples. The results show good coefficient with commercial ELISA kit (R2 = 0. 9424). The assay was sensitive, fast and accurate, which proved to be suitable for the screening of cereal samples for the presence of OTA.

  9. 基于单光子计数的半自动化学发光免疫分析仪的研究%The Research of A Semi-automatic Chemiluminescence Immunoassay Analyzer Based Photons Counting

    Institute of Scientific and Technical Information of China (English)

    罗刚银; 王弼陡; 唐玉国; 张昕

    2012-01-01

    A new semi-automatic chemiluminescence immunoassay analyzer was developed with the technology of photons counting. The analyzer used PMT as the detector of the weak fluorescence with photons counting, and used the measuring chamber and the optical system to improve the fluorescence collection. The number of FSH(3mIU/ml)'s photons is on an average of 1 610.8,115 307. 4 and 149 500. 8 respectively, which were tested by the photons counter without the optical system, with the parabolic reflector and with the optical system in turn. Obviously, the last two photons counters raised the photonic collection efficiency by 25. 86% and 63.19% respectively. The veracity of the analyzer was increased by using the measuring chamber and the optical system.%研究了一种基于单光子计数技术的新型半自动化学发光免疫分析仪.利用光电倍增管(PMT,Photomultiplier)作为微弱荧光的检测元件,采用单光子计数技术来测量微弱荧光光强,并且使用自主设计的测量暗室和聚光光路来提高光子采集效率.以浓度为3 mIU/ml的卵泡刺激素(FSH)为例,分别用不加聚光光路、单独加抛物面反射器、加了聚光光路测量其反应后的光子数值,所得光子数平均值分别是91 610.8、115 307.4、149 500.8,单独加抛物面反射器和加上聚光光路后的光子采集效率比不加聚光光路时分别提高了25.86%和63.19%.设计的新型半自动化学发光免疫分析仪采用自主研发的测量暗室和聚光光路,极大的提高了测量结果的准确性.

  10. 40 CFR 1065.270 - Chemiluminescent detector.

    Science.gov (United States)

    2010-07-01

    ... algorithms that are functions of other gaseous measurements and the engine's known or assumed fuel properties. The target value for any compensation algorithm is 0.0% (that is, no bias high and no bias...

  11. Content Determination of Rutin in Rutin Tablets by Luminol-potassium Hypermanganate Chemiluminescence Method%鲁米诺-高锰酸钾化学发光法测定芦丁片中芦丁的含量

    Institute of Scientific and Technical Information of China (English)

    白瑞霞; 宋素异

    2013-01-01

    OBJECTIVE: To establish a novel, rapid and sensitive method for the determination of rutin. METHODS: Rutin was found to enhance the chemiluminescence (CL) signal of luminol-potassium hypermanganate system in alkaline medium. Based on flow injection (FI), a new FI-CL method was developed for the determination of rutin. The concentrations of reagents were lu-minol (2.0×10-5 mol/L), potassium hypermanganate (8.0xl0~5 mol/L), sodium hydrate (0.05 mol/L); the flow rates of pumps were set at 2.12 ml/min (P1) and 3.45 ml/min (P2) with negative high voltage of 600 V and sampling time of 15 s. RESULTS: The linear range of rutin was 2.0×l0-9-2.0×l0-7 g/ml (r=0.999 1)with average recovery of 101.57% (RSD=1.69,n=3). The detection limit was 8.3×l0-10 g/ml. CONCLUSIONS: The method is convenient, rapid and sensitive, and can provide a new method for the quality control and product detection of rutin.%目的:建立一种快速、灵敏地测定芦丁含量的新方法.方法:利用在碱性条件下,芦丁对鲁米诺-高锰酸钾体系有强烈的增强作用的原理,结合流动注射技术,建立了流动注射化学发光法检测芦丁的新方法;反应试剂的浓度分别为:鲁米诺(2.0×10-5mol/L)、高锰酸钾(8.0× 10-5 mol/L)、氢氧化钠溶液(0.05 mol/L),主泵(P1)、副泵(P2)流速分别为2.12、3.45 ml/min,光电倍增管负高压为600V,采样时间为15s.结果:芦丁的检测质量浓度线性范围为2.0×10-9~2.0×10-7 g/ml(r=0.999 1),检出限为8.3×10-10g/ml,平均加样回收率为101.57%,RSD= 1.69%(n=3).结论:该方法简单、快速、灵敏性高,为芦丁药物的质量控制和体内分析提供了一种新的检测方法.

  12. 不同化学发光分析系统在皮质醇检测中的性能评价与比较%Performance evaluation and comparison of different chemiluminescence immunoassay systems in detection of cortisol

    Institute of Scientific and Technical Information of China (English)

    陈翊; 李颖能; 陈焕辉; 林涛; 汤勇才

    2016-01-01

    Objective To compare the analytical performance and results correlation of cortisol detection by the Beckman Access 2 Immunoassay System chemiluminescence system and the Roche COBAS E 411 electrochemical luminescence system .Methods The precision ,functional sensitivity ,reference range ,positive coincidence rate and negative coincidence rate of both Beckman Access 2 Immunoassay System and Roche COBAS E 411 systems for detecting cortisol were evaluated according to the method of ISO15198 .Results Both Beckman Access 2 Immunoassay System and Roche COBAS E 411 system exhibited better precision ,the functional sensitivity conformed to the lower limit of clinical detection set by the manufacturers ,after establishing the reference range of cortisol by the laboratory ,the cortisol values detected by these two systems were performed the linear regression analysis , the results indicated that the correlation between them was good ,the linear regression coefficient r2 =0 .977 ,and the positive and negative coincidence rates were 100% .Conclusion The two analytical systems have excellent performance for cortisol ,which all meet the clinical detection requirements .The methodological comparison shows good correlation between them ,and the detection re‐sults could be cited between these two systems by the regression equation .%目的:比较贝克曼Access 2 Immunoassay System化学发光免疫分析系统及罗氏COBAS E 411电化学发光免疫分析系统对皮质醇的分析性能与检测结果相关性。方法按照ISO15189中方法评估贝克曼及罗氏分析系统对皮质醇检测的精密度、功能灵敏度、参考范围、方法学、阳性符合率、阴性符合率。结果贝克曼与罗氏分析系统在皮质醇检测中精密度良好,功能灵敏度符合各自厂商设定的临床检测的下限,实验室自建参考范围后,将两分析系统对皮质醇的测定值进行线性回归分析,二者间相关性良好,线性回归系数 r2

  13. MnO_2纳米溶胶-甲醛化学发光体系及其分析应用研究%Chemiluminescence of Nano-Colloidal MnO2 with Formaldehyde and Its Analytical Application

    Institute of Scientific and Technical Information of China (English)

    杜建修; 王虹

    2012-01-01

    Water-soluble forms of colloidal MnO2 were prepared by the chemical reduction of KMnO4 with Na2S203 under neutral aqueous condition. The as-prepared colloidal MnO2 solution is dark-brown, trans- parent, stable, and possesses the maximum absorption peak at 375 nm and an average diameter of 40 nm. The as-prepared nano-colloidal MnO2 was found to react with formaldehyde to generate weak chemiluminescence (CL) under acidic condition. The effects of more than 30 pharmaceuticals on the nancolloidal MnO2-formaldehyde CL system were tested. Pharmaceuticals including phenothiazines and aminoethanethiols were observed to enhance the CL signal significantly. The experimental conditions were well optimized and the analytical figures for five phenothiazines and four aminoethanethiols were presented. The method was validated by the analysis of perphenazine in tablets and chlorpromazine hydrochloride in swine feed. The CL reaction mechanism was discussed by the study of CL spectra, fluorescence spectra, UV-vis absorption spectra, and other experiments. All of CL reactions had the same maximum emission wavelength about 640 nm, which suggested that the CL emitter was independent of analytes. The CL signal was inhibited obviously by single-state oxygen scavengers, sodium azide and 1,4-diazabicyclo[2,2,2]octane, indicating that single-state oxygen dimer was the potential CL emitter for the present CL reaction.%Na2S2O3在中性水溶液中还原KMnO4可制备得到暗棕色的可溶性MnO2溶胶.所制备的MnO2溶胶透明、稳定,最大吸收波长位于357 nm处,平均粒径约40 nm.研究发现,所制备的MnO2纳米溶胶在酸性介质中与甲醛反应可产生弱的化学发光.考察了近30种药物分子在MnO2纳米溶胶-甲醛体系中的化学发光行为.结果表明,吩噻嗪类药物、氨基硫醇类药物等对该体系的化学发光信号具有显著的增强作用.据此,建立了利用这一化学发光体系测定五种吩噻嗪类药物和四种氨基

  14. Silver nanoparticles enhanced luminol liquid chemiluminescence system for the determination of paracetamol by flow injection analysis%纳米银增敏鲁米诺液相化学发光分析法测定4-乙酰氨基酚的研究

    Institute of Scientific and Technical Information of China (English)

    杨猛; 马红燕; 辛建伟; 罗娟娟

    2011-01-01

    研究发现在碱性条件下,纳米银对鲁米诺-铁氰化钾液相化学发光体系发光信号具有明显的增敏作用,而4-乙酰氨基酚对该体系具有强烈的抑制作用。结合流动注射技术,建立了流动注射化学发光分析法测定对乙酰氨基酚的新方法。该方法测定对乙酰氨基酚的线性范围为9.0×10-12~1.0×10-10g/mL(0.9947)和1.0×10-10~7.0×10-9g/mL(0.9904),检出限(3σ)为7.9×10-12g/mL。对5.0×10-11g/mL和5.0×10-10g/mL对乙酰氨基酚平行测定11次,其相对标准偏差为1.3%和1.3%。该方法用于片剂中对乙酰氨基酚的测定,回收率为97.0%~101.5%。%The experiments indicated that silver nanoparticles ( AgNPs ) could greatly enhance the chemiluminescence intensity of the luminol-K3Fe(CN)6 system in sodium hydroxide medium, but paracetamol could inhibit the chemiluminescence signals significantly. On the basis of the inhibition, a novel flow-injection chemiluminescence method for the determination of paracetamol was proposed. The experimental conditions that influence the chemiluminescence intensity were carefully studied and optimized. Under the optimum conditions, the calibration graph for paracetamol was obtained. The linear range for the determination of paracetamol was from9 × 10T-12 to 1.0 × 10-10g/mL(0.9947) and 1.0 × 10-10 to 7.0 × 10-9G/mL(0. 9904) with a detection limit of 7.9 × 10-12g/mL. The relative standard deviations for 11 repeated measurements of 5.0 × 10-11 g/mL and 5.0 × 10-10 g/mL paracetamol were 1.3% and 1.3%, respectively. Possible interferences from major excipients in Pharmaceuticals and other related compounds were also studied. Contents of paracetamol in two locally available Pharmaceuticals were analyzed. The method has the merits of rapidness, simplicity, high sensitivity, wide linear range and has been used for the analysis of paracetamol in commercial tablets with recoveries in the range from 97.0% to 101.5%.

  15. The difference of ELISA and chemiluminescence method in detection of HIV-1/HIV-2 antibody,syphilis antibody and hepatitis C antibody on umbilical cord blood%ELISA法和化学发光法对脐血中HIV-1/HIV-2抗体、梅毒抗体和丙肝抗体的检测比较

    Institute of Scientific and Technical Information of China (English)

    王政

    2014-01-01

    目的:分析和比较临床上采用酶联免疫吸附法(ELISA)以及化学发光法检测脐血中HIV-1/HIV-2抗体、梅毒抗体以及丙肝抗体的结果。方法:2011年8月-2014年8月收集脐血1147份,对其临床数据进行回顾性分析,分别采用酶联免疫吸附以及化学发光法对其中的HIV-1/HIV-2抗体、梅毒抗体和丙肝抗体进行检测,并对结果进行分析。结果:1147份脐血中酶联免疫吸附方法检测 HIV-1/HIV-2抗体、梅毒抗体和丙肝抗体阳性率分别为0.61%(7/1147)、0.87%(10/1147)、1.13%(13/1147),而化学发光法检测HIV-1/HIV-2抗体、梅毒抗体和丙肝抗体阳性率分别为0.78%(9/1147)、0.96%(11/1147)、1.39%(16/1147),并且将购买的抗体标准品进行梯度稀释。检测结果表明,3种抗体稀释到10 pg/mL,化学发光法检测3组抗体均为阳性,酶联免疫吸附方法仅梅毒抗体为阳性,而HIV-1/HIV-2抗体、丙肝抗体的检测结果均为阴性。结论:本研究结果表明,临床上采用化学发光发对脐血中HIV-1/HIV-2抗体、梅毒抗体和丙肝抗体检测的灵敏性高于ELISA方法,因此在临床检测过程中建议采用化学发光发进行检测。%Objective:To analyze and compare the difference of enzyme linked immunosorbent assay(ELISA) and chemiluminescence method in detection of HIV-1/HIV-2 antibody,syphilis antibody and hepatitis C antibody on umbilical cord blood.Methods:1 147 units of cord blood were collected in our hospital from August 2011 to August 2014.The clinical data were retrospectively analyzed,using enzyme linked immunosorbent assay and chemiluminescence method respectively to detecte HIV-1/HIV-2 antibody,the syphilis antibody and hepatitis C antibody,then we analyzed the results.Results:Among those 1147 units of cord blood,the positive rate of HIV-1/HIV-2 antibody,syphilis antibody and HCV antibody were 0.61%(7/1147),0.87%(10/1147) and 1.13% (13/1147) respectively by using enzyme linked

  16. 非水毛细管电泳-化学发光法测定食品包装材料中酚类环境激素%Determination of Phenolic Environmental Estrogens in Food Packing Materials by Nonaqueous Capillary Electrophoresis-Chemiluminescence

    Institute of Scientific and Technical Information of China (English)

    肖全伟; 黎源倩; 邹晓莉

    2009-01-01

    建立食用包装材料中的双酚A、壬基酚等多种环境激素的非水毛细管电泳-化学发光分析方法.食品包装材料样品浸出物中的双酚A、烷基酚等环境激素经衍生剂DIB-Cl衍生后,经过非水毛细管电泳分离后,分别与过氧草酸酯化学发光反应体系作用,光信号经过光电倍增管接收放大后被检测.以雌二醇(17β-E2)为内标,以相对迁移时间定性,相对发光强度比定量,内标校准曲线法测定样品浸出物中待测物的含量.对影响非水毛细管电泳分离如溶剂组成和比例、电解质浓度、温度、乙酸浓度、电泳电压等条件进行了优化.同时对化学发光体系也进行了优化.4-叔丁基酚、双酚A、4-叔辛基酚、4-壬基酚和四溴双酚 A分别在0.0095~3.0 mg/L,0.0087~3.0 mg/L,0.0085~3.0 mg/L,0.011~3.0 mg/L和0.009~3.0 mg/L范围内线性良好,r>0.9947.相对迁移时间和相对峰高的RSD分别为0.88%~2.96%和 6.54%~8.57%.加标回收率为86.7%~98.8%.对5种常见的食品包装材料样品进行了测定,所建立的方法简便、快速、灵敏,适合于食品包装材料中酚类环境激素的检测.%Nonaqueous capillary electrophoresis-chemiluminescence (NACE) method was developed for the determination of five phenolic environmental estrogens including bisphenol A (BPA),4-nonylphenol(4-NP) ,4-tert-octylphenol(4-tOP),4-tert-butylphenol(4-tBP) and tetrabromobisphenol A(TBBPA) in food packaging material. Target compounds in soaking solution of food packaging material samples were separated by NACE-CL after derivatization with 4-(4,5-diphenyl-lH-imidazol-2-yl) benzoyl chloride ( DIB-Cl) ,reacted with per-oxyoxalate chemiluminescence system,and then detected through magnifying light signal with photomultiplier. With 17β-E_2 as internal standard,qualitative and quantitative analysis of the target compounds were performed by relative migration time and the ratio of relative chemiluminescence intensity

  17. 应用FITC系统建立非均衡竞争游离三碘甲腺原氨酸(FT3)化学发光法%Detection of free triiodothyronine in human serum by non-equilibrium competitive chemiluminescence immunoassay using FITC system

    Institute of Scientific and Technical Information of China (English)

    孔海霞; 刘萍; 田云霞; 宋启超; 王世鑫; 翁亚光

    2012-01-01

    目的 制备抗人T3多克隆抗体,应用异硫氰酸荧光素(fluorescein isothiocyanat,FITC)系统开发新型非均衡竞争技术,建立一种能广泛应用于临床检测游离三碘甲腺原氨酸(free triiodothyronine,FT3)的方法.方法 以T3-牛血清白蛋白(bovin serum albumin,BSA)为抗原免疫新西兰大白兔,制备抗人T3多克隆抗体,亲和层析法纯化并联接辣根过氧化物酶(horse radish peroxidase,HRP).用FITC标记T3-类似物.采用抗FITC抗体包被发光板,FITC-T3类似物与抗FITC抗体结合形成固相抗原,通过非均衡竞争法原理,固相抗原与血清中FT3竞争结合抗T3抗体-HRP,通过标准曲线计算FT3浓度,建立方法学评价并与直接用T3-牛血清γ球蛋白(Bovine serum gamma globulin,BGG)包被(非FITC系统)的检测系统相比较.将本方法应用于临床120份血清标本的检测,定量结果与德国罗氏2010电化学发光全自动免疫分析系统( Elecsys 2010)定量结果进行比较.结果 抗体经酶联免疫吸附实验证明对T3、具有高度特异性.应用FITC系统建立的非均衡竞争化学发光免疫分析标准曲线的线性R>0.99,线性范围0.25~50 pg/ml,分析灵敏度为0.25 pg/ml,精密度测试批内、批间变异系数CV<5%,检测结果优于非FITC系统的检测.对于临床血清标本的检测,其定量结果与德国Elecsys 2010定量试剂盒的定量结果具有很好的相关性,相关系数r =0.962 2,两者的测定值无显著性差异.结论 制备的抗人T3多克隆抗体特异性强,以FITC系统为平台建立的非均衡竞争FT3检测化学发光法具有精密性好、灵敏度高、稳定性强,有良好的准确性,达到临床测定的需求,可代替进口化学发光产品用于临床样本的检测.%Objective To prepare a polyclonal antibody ( mAb) against human triiodothyronine ( T3) and to establish a new method of non-equilibrium competitive chemiluminescence immunoassay ( CLIA) with FITC system for clinical

  18. Chemiluminescence Immunoassay for Quantitative Analysis of Prostate Specific Antigen Complexed toα1-Antichymotrypsin in Human Serum%化学发光免疫分析法检测血清中前列腺特异抗原复合物

    Institute of Scientific and Technical Information of China (English)

    周友俊; 李娇霞; 程绘珺; 杨乔芬; 何美琼; 郭丽萍; 邓智勇

    2016-01-01

    Eight mouse hybridoma cell lines which stably secreted monoclonal antibodies ( McAbs ) against human prostate-specific antigen-α1-antichymotrypsin complex ( PSA-ACT ) were obtained through hybridoma technique. After purification, the immunological characters of 8 McAbs were identified and classified by epitopes analysis through indirect enzyme-linked immunosorbent assay ( ELISA) . A pair of McAbs was chosen from above 8 McAbs, based on which a highly sensitive, simple and rapid chemiluminescence enzyme immunoassay ( CLEIA) was developed for determination of PSA-ACT in human serums using the lumino-H2 O2 reaction catalyzed by horseradish peroxidase ( HRP) as the chemiluminescence system. Several experiment factors such as coating buffer, coating concentration, dilution ratio of PSA-ACT-HRP complex, incubation time, immunoreaction protocol and chemiluminescence reaction time were optimized. The results showed that the linear range of the proposed method for PSA-ACT determination was 0-40 ng/mL (R2=0. 9943), with the detection limit of 0. 53 ng/mL. The inter-assay relative standard deviations (RSDs) were 4. 6%-6. 6%, and intra-assay RSDs were 5 . 7%-8 . 0%. The recoveries of PSA-ACT at three spiked levels in serum samples were 95. 4%-104. 2%. The proposed method exhibited a cross-reactivity of 0. 6% with free-PSA. The proposed method is stable, sensitive, rapid and simple, and provides a foundation for the development of PSA-ACT CLEIA kit and shows great value in clinical auxiliary diagnosis of prostate cancer.%采用杂交瘤技术构建抗前列腺特异抗原-α1-抗胰凝乳蛋白酶( Prostate specific antigen-α1-antichymot-rypsin complex,PSA-ACT)单克隆抗体(单抗)细胞株,共获得8株稳定分泌PSA-ACT单抗的细胞株,纯化后进行免疫特性鉴定并做单抗配对。选择其中一对单抗,采用辣根过氧化物酶( HRP)催化鲁米诺( lumino)-H2 O2化学发光体系,建立了测定人血清中PSA-ACT浓度的化

  19. The study of anti-oxidation of red ginseng、shengshai ginseng、American ginseng and the protective effect on DNA with chemiluminescence%化学发光法研究三种参对DNA损伤的保护作用

    Institute of Scientific and Technical Information of China (English)

    方荣美; 何品刚; 李晓军; 方禹之

    2000-01-01

      利用CuSO4-Phen-Vc-H2O2-DNA化学发光体系研究了红参、生晒参、西洋参对DNA氧化损伤的保护作用。通过实验发现,对DNA的保护作用为红参优于生晒参、生晒参优于西洋参。因为人参(红参和生晒参)所含的有效成分三醇皂甙的量比西洋参多,而红参由于加工过程不同,产生新的抗氧化剂如maltol和水杨醛。%  In this paper, we've studied the protective effects of three kinds of ginseng(red ginseng、shengshai ginseng and American ginseng) on DNA against the oxidative damage in CuSO4-Phen-Vc-H2O2-DNA chemiluminescence mixture. We find that the protective effect of red ginseng's extract is the best. The protective effect of shengshai ginseng is better than that of American ginseng.The reason is that the amount of effective component(sancun zaodai) in ginseng(including red ginseng and shengshai ginseng) is larger than that in American ginseng, and because of different process, new antioxidants such as maltol and salicylaldehyde are formed in red ginseng.

  20. Validation of the extension of the range of application and of the single system of injection for the determination of total nitrogen in petroleum and its derivatives by chemiluminescence; Validacao da ampliacao da faixa de aplicacao e do sistema unico de injecao para a determinacao de nitrogenio total em petroleos e derivados por quimiluminescencia

    Energy Technology Data Exchange (ETDEWEB)

    Santos, Maria de Fatima Pereira dos [Fundacao Gorceix, Ouro Preto, MG(Brazil)]. E-mail: santos@cenpes.petrobras.com.br; Tamanqueira, Juliana Boechat [Universidade Federal Fluminense, Niteroi, RJ (Brazil); Magalhaes, Julio Cesar Dias [PETROBRAS, Rio de Janeiro, RJ (Brazil). Centro de Pesquisas. Avaliacao de Petroleo; Oliveira, Elcio Cruz de [Transpetro, Rio de Janeiro, RJ (Brazil). Logistica e Planejamento; Vaitsman, Delmo Santiago [Universidade Federal, Rio de Janeiro, RJ (Brazil). Inst. de Quimica. Dept. de Quimica Analitica

    2003-12-01

    With the objectives of using a single system of injection and of extending the range of application for the method ASTM D4629/02 'Total Nitrogen in Petroleum Derivatives by the System of Injection with a Syringe and Detection by Chemo- Luminescence', it was studied a procedure by statistical validation with the objective of guaranteeing the analytical reliability of the assay and allowing the inclusion of samples of petroleum and heavy derivatives in one single methodology. The determination of total nitrogen for petroleum and derivatives is traditionally assayed using the method of Kjeldahl - a time-consuming methodology that requires a large amount of time for giving the final result, at the same time that is not recommended for concentrations below 0.1%w/w, which does not meet the requirements for the specifications of the product, in the petroleum industry. An alternative for the method of Kjeldahl is the pyro-chemo luminescence, which allows the achievement of more repetitive results for total nitrogen. In the detection of nitrogen, the technique combines the reliability of oxidative combustion with the sensitivity of chemiluminescence. Therefore, it was developed a protocol of validation in the methodology ASTM D4629/02 for the validation of the extension of the range of application and for the evaluation of the performance of the equipment in analytical conditions, according to the calibration curve. (author)

  1. The Fabrication and Its Analytical Applications of Paper-Based Microfluidics for The Detecting of Glucose and Uric Acid with Chemiluminescent Image%纸芯片制作及其在化学发光法检测葡萄糖和尿酸中的应用

    Institute of Scientific and Technical Information of China (English)

    王方方; 陈锦; 何治柯

    2011-01-01

    Microfluidic paper-based analytical devices (μPADs), a new class of point-of-care diagnostic devices, provide an exciting platform for the rapid, low-cost, and technically simple multiplexed bioassays. In this work, photolithography was used to pattern the paper. An equation was obtained to describe the relationship between the volume of samples loaded and the volume of the channels. The analytical application was demonstrated by the detection of glucose and uric acid based on the enzyme induced chemiluminescence of luminol system. Under the optimized conditions, the chemiluminescence (CL) intensity is linear with glucose concentration over the range of 2. 0× 10-4~9. 0× 10-4 mol/L with a limit of detection (LOD) of 5. 0× 10-5 mol/L. The LOD for glucose was found to be substantially lower than that of the electrochemical method (5.0 × 10-4 mol/L) onμPADs. In the same principle, the CL intensity is linear with uric acid concentration over the range of 1. 0× 10-4~1.2× 10-3 mol/L with a LOD of 6. 0× 10-5 mol/L. The LOD for uric acid was found to be two orders of magnitude lower than that of the electrochemical method (1. 38 × 10-3 mol/L) on μPADs. 1D and 2D μPADs have also assembled into 3DμPADs to detect glucose and uric acid simultaneously.%纸芯片是一类新型的个性化诊断器件,可快速、廉价、简便地进行多组分分析.采用光刻法制备了一维和二维纸芯片(μPADs),并对不同通道芯片检测所需的样品体积进行理论估算.同时利用葡萄糖氧化酶-葡萄糖反应产生过氧化氢诱导鲁米诺化学发光,在纸芯片上检测葡萄糖,线性范围为2.0×10-4~9.0×10-4mol/L,检出限为5.0×10-5mol/L,比纸芯片上电化学检测葡萄糖(5.0×10-4mol/L)低一个数量级.利用同样的原理进行了尿酸检测,线性范围为1.0 × 10-4~1.2×10-3 mol/L,检出限为6.0×10-5mol/L,检测0.5 mmol/L葡萄糖和0.5 mmol/L尿酸的相对标准偏差分别为2.68%(n=11)和4.59%(n=11).将一维、

  2. Comportamento do método quimioluminescente-ELISA em relação a resultados considerados discordantes por meio de três técnicas convencionais para diagnóstico da doença de Chagas Behavior of the chemiluminescent ELISA method in relation to results considered discordant via three conventional techniques for diagnosing Chagas disease

    Directory of Open Access Journals (Sweden)

    Cláudia Regina De Marchi

    2007-02-01

    Full Text Available Quando utilizadas, em conjunto, a hemaglutinação indireta, a imunofluorescência indireta e ELISA para diagnóstico sorológico da doença de Chagas por vezes ocorrem resultados considerados discordantes, por não haver concordância entre o que indicam essas técnicas. A disponibilidade do método quimioluminescente-ELISA permitiu executá-lo com 200 soros que examinados pelos três testes citados que motivaram a obtenção de resultados discordantes. Com o método quimioluminescente-ELISA sucederam 193 negativos e sete positivos. O emprego desse novo procedimento trouxe mais um subsídio para compreensão do assunto, mas avanço mais concreto dependerá de documentação com soros de pessoas infectadas ou não pelo Trypanosoma cruzi conforme comprovação parasitológica.When indirect hemagglutination, indirect immunofluorescence and enzyme-linked immunosorbent assay are used together for serologically diagnosing Chagas disease, results that are considered discordant sometimes occur because there is disagreement between what these tests indicate. The availability of the chemiluminescent ELISA method enabled tests on 200 serum samples that had previously produced discordant results from the three abovementioned methods. CL-ELISA revealed that 193 of these samples were negative and seven were positive. The use of this new procedure provides further support for understanding this subject, but more concrete advances will depend on documentation with blood analyses from people previously demonstrated to be unquestionably infected or uninfected with Trypanosoma cruzi.

  3. Development of an indirect competitive chemiluminescence immunoassay for determining ractopamine residues in swine meat and urine%检测猪尿、猪肉中莱克多巴胺的化学发光间接竞争免疫分析方法的建立

    Institute of Scientific and Technical Information of China (English)

    王权; 赵新宇; 蒋蔚; 陈永军; 刘迎春; 宋宁宁; 石金磊

    2013-01-01

    将莱克多巴胺(RAC)与戊二酸酐反应,用混合酸酐法使反应产物与牛血清白蛋白(BSA)偶联生成免疫原,并采用淋巴细胞杂交瘤技术制备RAC单克隆抗体,建立检测猪尿、猪肉中莱克多巴胺化学发光间接竞争免疫分析法.结果显示:飞行质谱法测得RAC-戊二酸单酯分子量为416.2,RAC-戊二酸单酯与BSA偶联比为3.4∶1;RAC单克隆抗体最佳稀释倍数为l:8×104,包被抗原最佳质量浓度为0.5 μg/mL;标准曲线呈线性,回归方程为Y=-0.188 2x+0.392 2,R2=0.993 4,IC50值为0.267 ng/mL;与RAC类似化合物及其他药品无明显的交叉反应.尿样回收率为76.8% ~90.8%,批内变异系数为6.8% ~11.5%,批间变异系数为7.2% ~13.8%;猪肉样回收率为75.2%~ 87.4%,批内变异系数为7.1%~11.6%,批间变异系数为8.6%~14.1%.结果表明:用RAC-戊二酸单脂与BSA偶联物作免疫原成功地研制了针对RAC的特异性抗体,以单抗为基础建立了检测猪尿、猪肉中莱克多巴胺化学发光间接竞争免疫分析法,为进一步研制快速筛选检测试剂盒奠定了基础.%The experiment was conducted to establish an indirect competitive chemiluminescence immunoassay for determining ractopamine (RAC) residues based on monoclonal antibody (McAb). Ractopamine was coupled to bovine serum albumin (BSA) by mixture anhydrides method. The RAC-BSA conjugate was used as immunogen and McAb was prepared using lymphocytes hybridoma technology. The indirect competitive chemiluminescence immunoassay based on McAb was developed for detection and estimation of RAC residues in swine meat and urine. The molecular weight of RAC-glutaricacid mono-lipid was 416. 2 by MALDI TOF-MS/MS. The couple ratio of RAC-BSA was about 3. 4 : 1. The optimal concertration of the coating antigen (RAC-OVA) and that of McAb against RAC were 0. 5 μg/mL and 1 : 80 000, respectively. Linear detection of standard curve covered a range from 0. 0lng/mL to

  4. Analysis of Volatile Sulfur Compounds in Waste Gases by Gas Chromatography with a Sulfur Chemiluminescence Detector%硫化学发光检测器-气相色谱法测定废气中挥发性硫化物

    Institute of Scientific and Technical Information of China (English)

    韩丛碧; 李凌波

    2012-01-01

    采用硫化学发光检测-宽口径石英毛细管柱气相色谱法,建立了废气中羰基硫、硫化氢、二硫化碳、甲硫醇、乙硫醇、甲硫醚、乙硫醚、丙硫醇、异丙硫醇、噻吩及二甲二硫等11种挥发性硫化物的分析方法.硫化学发光检测器的灵敏度、选择性及线性范围均优于传统的火焰光度检测器.进样体积为0.2 ml时,硫化物的检出限为0.03~0.1 mg/m3.实际样品5次测定的相对标准偏差小于5.1%,加标回收率为83.7%~108.8%.测定了某炼油厂酸性水罐尾气、污油罐尾气及瓦斯气中挥发性硫化物,为其恶臭控制与治理提供检测手段和基础数据.%Sulfur chemiluminescence detector-vast capillary gas chromatography has been conducted on the analysis of volatile sulfur compounds in waste gases. The recoveries of hydrogen sulfide, carbon disulfide, methyl mercaptan, ethyl mercaptan, carbon disulfide,dimethyl sulphide,diethyl sulphide, n-propyl merrcaptan, i-propyl merrcaptan, thiophene and dimethyl disulfide were from 83. 7% to 108. 8% , the relative standard deviations were less than 5. 1% . When inject volumn was 0. 2 ml, the minimum detection levels of the above-mentioned volatile sulfur compounds varied between 0.03 mg/m3and 0. 1 mg/m3 Volatile sulfur compounds in waste gases from sour water tanks and oil tanks in an refinery and Gas were analyzed.

  5. 鲁米诺-铁氰化钾流动注射化学发光体系测定饮用水中ɑ-茄碱%Determination of a-solanine in drinking water by flow-injection chemiluminescence system of luminol and potassium ferricyanide

    Institute of Scientific and Technical Information of China (English)

    朱定波; 马强; 燕廷; 滕久委; 唐书泽

    2012-01-01

    In order to establish a new method for rapid determination of a-solanine,a flow-injection chemiluminescence(FI-CL) system of luminol and potassium ferricyanide was developed.Results indicated that the CL reaction betweeen luminol and potassium ferricyanide was significantly reduced by a-solanine.An excellent linear range for a-solanine determination was 8.0×10-4~1.0×10-1mg/mL under the optimal condition.The limit detection of a-solanine was 6.09×10-5mg/mL and relative standard deviation for the determination was 2.1%(n=11).This method showed the advantages of simple operation,high degree of automation,easy to run on-line and real-time warning detection,and could be used as a rapid method for determination of a-solanine in drinking water.%建立一种快速测定ɑ-茄碱的新方法,利用鲁米诺-铁氰化钾化学发光体系,结合流动注射技术,对ɑ-茄碱进行测定。实验结果表明,ɑ-茄碱对鲁米诺-铁氰化钾体系发光反应有明显的抑制作用,在最佳实验条件下,该方法的线性范围为8.0×10-4~1.0×10-1mg/mL,检出限为6.09×10-5mg/mL,RSD为2.1%(n=11)。该方法具有操作简单、自动化程度高、成本低廉、容易实现在线检测以及即时预警等优点,可用于饮用水中ɑ-茄碱的快速检测。

  6. HBV serological test results comparing the use of chemiluminescence and en-zyme-linked immunosorbent assay of%乙肝病毒血清学检验采用化学发光法与酶联免疫法的效果对比

    Institute of Scientific and Technical Information of China (English)

    张怡莎

    2014-01-01

    目的:探讨化学发光免疫分析技术(ECLIA)和酶联免疫吸附试验(ELISA)在乙肝病毒血清学检验中的应用效果。方法选取2012年1月-2014年1月在我院就诊的疑似乙肝患者150例,分离血清后分别应用ELISA和ECLIA进行检测,比较两种检测方法的效果。结果ELISA和ECLIA检测出HBsAg阳性分别63例和82例,两者比较差异显著(P<0.05),同时ECLIA法检测的批内CV和批间CV的重复性均明显高于ELISA法(P<0.05)。结论与ELISA检验法比较,ECLIA法具有更高的HBsAg阳性检出率,且能进行精确的定性定量检测,值得临床推广应用。%Objective To investigate chemiluminescence immunoassay (ECLIA) and enzyme-linked immunosorbent assay (ELISA) in HBV serological test the application results. Methods Select suspected hepatitis B patients from January 2012 to January 2014 in our hospital 150 cases, respectively, after the application of separation of serum ELISA and ECLIA detection, the effect of two detection methods.Results ELISA and ECLIA detection of HBsAg positive 63 cases and 82 cases respectively,the difference was significant (P<0.05),while intra-assay and inter-assay CV CV ECLIA assay reproducibility were significantly higher than the ELISA method(P<0.05). Conclusion Compared with ELISA test method, ECLIA method has higher HBsAg positive rate,and can be accurate qualitative and quantitative detection,worthy of clinical application.

  7. 化学发光法与 PCR法联合检测孕妇单纯疱疹病毒感染率分析%The detection of herpes simplex virus infection rate by jointly using chemiluminescence assay and PCR

    Institute of Scientific and Technical Information of China (English)

    倪雪梅; 朱巧英; 李宁; 成春岚

    2016-01-01

    Objective Detect the infection rate of herpes simplex virus (HSV ) by jointly using chemiluminescence assay and PCR ,and provides reference for clinical diagnosis .Methods The serum samples were collected from the pregnant women who had routine examination records in the hospital .Chemiluminescence assay was used to detect HSV IgM and IgG in those samples .Cervi‐cal secretions were collected from pregnant women with positive results and qualitatively tested for HSV DNA .Results The posi‐tive rate of HSV1 DNA was 0 .5% (7/1 422) ,the positive rate of HSV2 DNA was 1 .1% (16/1 422) .For pregnant women whose HSV IgM and IgG were both positive ,positive rate of HSV1 DNA was 0 .4% (4/1 008) and that of HSV2 DNA was 0 .6%(6/1 008);for those who only had HSV IgM positive ,the positive rate of HSV1 DNA was 0 .8% (1/130) ,and that of HSV2 DNA was 3 .1% (4/130);for those who only had HSV IgG positive ,the positive rate of HSV1 DNA was 0 .7% (2/284) ,that of HSV2 DNA was 2 .1% (6/284) .Among those three HSV antibody positive cases ,the difference in HSV1 DNA positive rate was not sta‐tistically significant(P>0 .05) ,while the difference in HSV2 DNA positive rate was statistically significant(P<0 .05) .Conclusion The test of HSV antibodies during pregnancy can be used as a routine test ,and HSV DNA test can be used as further test for those with HSV antibody positive ,which could improve the accuracy of diagnosis .Early screening ,detection ,and treatment are im‐portant for pregnant women with HSV infection .%目的:对化学发光法与聚合酶链反应(PCR)联合检测孕妇单纯疱疹病毒(HSV)的感染率进行分析,为临床诊断提供依据。方法采集该院孕期常规建卡的孕妇血清,用化学发光法检测血清HSV IgM与IgG抗体水平;对其中任一项结果阳性者取宫颈分泌物进行 HSV DNA 定性检测。结果 HSV1 DNA 阳性率为0.5%(7/1422),HSV2 DNA 阳性率为1.1%(16/1422)。HSV Ig

  8. Chemiluminescent examination of abiotic oxidative stress of watercress.

    Science.gov (United States)

    Beals, Christopher; Byl, Thomas

    2014-04-01

    Watercress (Nasturtium officinale) is an aquatic plant that readily bioaccumulates heavy metals that may be found in contaminated aquatic systems. Toxic effects of contaminants on the physiological processes cause changes in oxidase enzymatic activity in watercress, which can be measured with a luminometer. The luminometer uses the reaction produced when peroxidases break down hydrogen peroxide into water and an oxygen radical. The resulting oxyradical binds to and oxidizes phenolic groups, producing a measureable luminescent reaction. Nasturtium officinale plants were exposed to 3 different concentrations of heavy metals, including lead, nickel, copper, and manganese for 24 h, 48 h, and 72 h. Aquatic exposure to the 4 heavy metals caused a significant increase in oxidative enzyme production. Fluorometric and morphometric measurements were also conducted to compare plant stress with the oxidative enzyme analyses. Fluorometric measurements performed on plants stressed by exposure to heavy metals revealed no significant decreases in photosystem II efficiency. Morphometric measurements of root length showed decreased root growth resulting from exposures to Ni, Cu, and Mn. PMID:24306856

  9. Synthesis of unstable cyclic peroxides for chemiluminescence studies

    Energy Technology Data Exchange (ETDEWEB)

    Bartoloni, Fernando H.; Oliveira, Marcelo A. de; Augusto, Felipe A.; Ciscato, Luiz Francisco M.L.; Bastos, Erick L.; Baader, Wilhelm J., E-mail: wjbaader@iq.usp.br [Universidade de Sao Paulo (USP), SP (Brazil). Inst. de Quimica. Dept. de Quimica Fundamental

    2012-11-15

    Cyclic four-membered ring peroxides are important high-energy intermediates in a variety of chemi and bioluminescence transformations. Specifically, a-peroxy lactones (1,2-dioxetanones) have been considered as model systems for efficient firefly bioluminescence. However, the preparation of such highly unstable compounds is extremely difficult and, therefore, only few research groups have been able to study the properties of these substances. In this study, the synthesis, purification and characterization of three 1,2-dioxetanones are reported and a detailed procedure for the known synthesis of diphenoyl peroxide, another important model compound for the chemical generation of electronically excited states, is provided. For most of these peroxides, the complete spectroscopic characterization is reported here for the first time. (author)

  10. Flow-injection Chemiluminescence Determination of Trace Calf Thymus DNA

    Institute of Scientific and Technical Information of China (English)

    Min ZHOU; Yong Jun MA; Xiao Yong JIN; Xiu Lan TENG; Zi Yu ZHANG; Hui CHEN

    2003-01-01

    A flow injection procedure for the determination of calf thymus DNA (CT DNA) overthe range 2.1x10-6~2.1×10-1 μg mL-1 is described, based on measurement of the enhancedchemiluminescence emission of rhodamine B-Ce(Ⅳ) system, activated by imidazole-HCl buffersolution. This method is highly sensitive, with the linearity range broadened to five orders ofmagnitude. It has been applied to determine CT DNA in synthetic sample with satisfactoryresults.

  11. Potassium permanganate-rhodamine B chemiluminescence for the determination of sulphite in food by pervaporation and flow injection%渗透汽化流动注射高锰酸钾-罗丹明B化学发光法测定食品中的亚硫酸盐

    Institute of Scientific and Technical Information of China (English)

    张红兵; 胡云霞; 连靠奇; 狄柯坪

    2013-01-01

    目的:结合渗透汽化和流动注射技术,建立一种高锰酸钾-罗丹明B化学发光测定食品中亚硫酸盐的新方法.方法:在酸性介质中,高锰酸钾氧化罗丹明B产生微弱的化学发光,SO2的存在可显著增强该体系的发光信号强度;且在一定范围内呈良好的线性关系;渗透汽化技术在酸性条件下将样品中的亚硫酸盐转化为SO2并分离出来,基于此,利用渗透汽化与化学发光联用技术进行食品中亚硫酸盐含量测定.结果:Na2 SO3浓度0.8 mg/L~4.0 mg/L范围内与发光强度呈良好的线性关系,检测限是0.2 mg/L.对2.0mg/L的Na2SO3标准溶液平行测定11次,平行测得值的RSD为4.2%,用于测定食品中亚硫酸盐含量,结果满意.结论;该方法可以用于测定食品中亚硫酸盐含量.%Objective:A chemiluminescence (CL) procedure was developed by pervaporation and flow injection of potassium permanganate-rhodamine B for determination of contaminated sulphite in food samples.Methods:The method is based on the oxidation of rhodamine B by permanganate solution in acid medium to give out weak CL signal,which is dramatically enhanced by sulphite which can be converted to sulphur dioxide and transported in pervaporation module.The CL signal is linear with sulphite concentration in a certain range.Results:Under optimum conditions,the CL emission showed linear with Na2SO3 in the concentration range of 0.8 mg/L ~ 4.0 mg/L,the detection limit was 0.2 mg/L.The relative standard deviation was 4.2% when the concentration of Na2SO3 was2.0 mg/L.Conclusion:The method is applied to the determination of sulphite in different food samples with satisfying results.

  12. 两台化学发光仪检测人生长激素的比对分析及偏倚评估%Comparative analysis and bias assessment of detecting results of human growth hormone using two chemiluminescence ap-paratus

    Institute of Scientific and Technical Information of China (English)

    杨方华; 邓芳梅; 孙丽芳

    2013-01-01

    Objective To explore the comparability of human growth hormone(hGH) detected by chemilumi-nescence apparatus of SIEM ENS Immulite 1000 and Beckman Coulter DXI 800 detection systems ,through methodol-ogy comparing and bias assessing .Methods According to Clinic and Laboratory Standrd Instritate (CLSI) EP9-A2 document ,at least 8 copies of different concentrations of fresh serum were collected everyday ,then we tested hGH with two detection systems for at least 5 days continuously .Every sample was detected repeatedly with obverse and reverse sequence ,then we recorded the results ,checked outlier ,observed linearity and data distribute ,and calculated correlation coefficient and linear regression equation .According to the regression equation ,we calculated hGH in the critical value of the expected bias and the expected bias of the 95% confidence interval ,and determined the bias ac-cepted or not .Results The correlation coefficient (r) of two detection systems in hGH was 0 .999 ,and the difference of mean results was negative bias .The bias was in the permissible error range ,and it was acceptable .Conclusion Methodology comparison and bias estimation of the same test items in different detection systems should be carried out regularly in order to ensure comparability and continuity of test results .%目的:对 Immulite 1000化学发光仪和 DXI 800化学发光仪两种不同检测系统检测人生长激素(hG H )进行方法比对及偏倚评估,探讨不同检测系统间测定结果是否具有可比性。方法依据美国临床和实验室标准化协会(CLSI)EP9-A2文件要求,每天至少收集8份不同浓度的新鲜血清,分别用两种检测系统测定hGH ,连续测定至少5d,每个标本均按正反序重复测定,记录测定结果,检查离群点,目测线性及数据分布,计算相关系数和直线回归方程,根据回归方程计算hG H在临界值处的预期偏倚和预期偏倚的95%可信区间并判断

  13. 总前列腺特异性抗原在化学发光免疫检测系统间的可比性研究%Comparability Research on Results of Total Prostate-specific Antigen Between Chemiluminescence Immunoassay Systems

    Institute of Scientific and Technical Information of China (English)

    周俊; 宋超

    2013-01-01

    目的 探讨化学发光免疫分析系统测定PSA结果的可比性,为该项目推广互认提供依据.方法 选择Roche与Abbott Architect作为研究对象,通过比较各台仪器的正常人参考值以及健康人群P50、P95和P975来考虑两者是否具有可比性.随后6个月内跟踪6台检测系统的室内质控、进行40份不同浓度新鲜患者血清的比对、结合4年内35个批次的室间质评数据,综合分析两款检测系统在整体上与个体间的可比性和差异情况,并对照澳大利亚室间质评的相关要求来讨论两款检测系统间结果是否可以推广互认.结果 两款检测系统具有接近的健康人群参考范围;同款检测系统之间具有较好的一致性;不同款检测系统间则表现出整体一致性优于个体之间的一致性,且随实验室不同而表现出不同的差异率,部分差异超出了相关要求.结论 基于稳定的实验室室内质控和规范的校准条件下,两款检测系统在低浓度的结果可以推广互认,不同实验室的同款检测系统也满足推广互认的要求,但不同款的检测系统之间的互认尚存在不确定性,需进一步验证.%Objective To investigate and analyze the comparability of serum prostate - specific antigen measurements between automated chemiluminescence immunoassay systems. It is valuable to provide the basis for the mutual approval between laboratories on results. Methods Six systems from Roche and Abbott Architect in six laboratories were involved in this research. Normal reference ranges of each laboratory were compared to confirm that both two brands of systems are comparable. We measured PSA concentrations in each of 40 serum samples during six months and compared the results using various statistical methods. EQA data from National Center for Clinical Laboratory were analyzed to show the comparability between analyzer groups. Evaluation standards of Australia were used to measure whether these

  14. Evaluation of detection performance of DXI800 chemiluminescence analyzer according to EP10-A2 document of CLSI%应用 CLSI EP10-A2文件对 DXI800化学发光仪性能评价的结果分析

    Institute of Scientific and Technical Information of China (English)

    杨方华; 杨宝瑞; 马东礼; 邓芳梅; 孙莉芳

    2014-01-01

    目的:应用CLSI EP10-A2文件初步评价DXI800化学发光仪的分析性能。方法严格按照 EP10-A2文件要求,连续5 d按特定顺序测定高、中、低浓度质控血清中的人生长激素(hG H )浓度,采用 Excel软件计算hG H测定的偏差、总不精密度及每天的多元回归分析参数(即截距、斜率、非线性、携带污染率及漂移度)。结果当质控血清hGH浓度为2.15、5.47、10.80 mmol/L时,hGH测定值与靶值间线性良好(Y =0.992X+0.083,r2=0.978);偏差分别为0.066、0.265、0.055 mmol/L ;总不精密度分别为6.57%、8.53%、4.56%;采用单样本 t检验,每个多元回归分析参数5 d的值与其预测值相比,差异均无统计学意义( P>0.05)。结论 DXI800化学发光仪测定hG H ,其偏差及总不精密度均在允许范围内,线性关系良好,携带污染率较低,稳定性良好,能满足临床及科研需求。%Objective To evaluate the detection performance of DXI800 chemiluminescence analyzer (DXI800 analyzer) according to EP10-A2 document of Clinic and Laboraroty Standard Institute (CLSI) .Methods According to EP10-A2 document ,human growth hormone (hGH) in quality control serum were continuously detected for five days with a certain order ,then the deviation ,total imprecision ,intercept ,slope rate ,non-linearity ,cross-contami-nation and drift were analyzed by using Microsoft Excellsoftware .Results The linearity between measured value and target value was relatively well (Y=0 .992X+0 .083 ,r2 =0 .978) when the concentrations of hGH were 2 .15 ,5 .47 and 10 .80 mmol/L ,with bias of 0 .066 ,0 .265 and 0 .055 mmol/L ,and total imprecision of 6 .57% ,8 .53% and 4 .56% ,respectively .Simple sample t test indicated that the difference of intercept ,slope rate ,non-linearity ,cross-contamination and drift was no statistically significant (P>0 .05) .Conclusion The deviation ,total imprecision ,in

  15. Performance evaluation of paramagnetic particles chemiluminescence micro-particle immunoassay for determination of serum vitamin B12%顺磁性微粒子化学发光免疫分析法检测血清维生素B12的性能评价

    Institute of Scientific and Technical Information of China (English)

    安崇文; 李海霞; 孟群; 胡建平; 徐向东

    2014-01-01

    目的:评估顺磁性微粒子化学发光免疫分析法( Chemiluminescence microparticle immunoassay,CMIA)检测血清维生素B12( Vitamin B12,VitB12)的分析性能。方法:应用美国临床和实验室标准化协会( CLSI) EP5-A2、EP15-A2、EP7-A2、EP6-A、C28-A3c方法评价CMIA检测VitB12的精密度、正确度、抗干扰性、分析测量范围(AMR)、临床可报告范围(CRR)、生物参考区间。采用美国国家标准技术研究所( National Institute of Standards and Technology,NIST)有证参考物质SRM 1955、美国病理学家协会(CAP)发放的室间质评物(K-C)、校准验证/线性评价物(LN5-B)评估CMIA系统检测VitB12正确度。结果:VitB12在108.84~874.43 pmol/L时,批内、批间精密度均小于厂家声明的标准。正确度验证显示测定有证参考物质NIST SRM 1955,结果符合验证要求;检测CAP室间质评物(K-C)、校准验证/线性评价物(LN5-B)显示,结果均符合CAP校准验证/线性评价误差界限所规定的标准,VitB12浓度在89~1057 pmol/L范围内通过线性验证,95%验证区间也包含其指定均值,相对偏差均小于卫生部临床检验中心室间质量评价标准( TEa:靶值±25%)。抗干扰性评估显示在TG≤20 mmol/L、Bil≤300μmol/L、Vit C≤1.5 g/L时对VitB12检测系统(CMIA)无显著干扰。 AMR验证判断最佳拟合方程为二元一次多项式,VitB12浓度在0~1107 pmol/L范围内存在线性关系。 CRR上限为110700 pmol/L,最大稀释倍数为100倍。生物参考区间验证显示本研究选择的参考个体VitB12水平符合厂家试剂说明书给定参考区间,女性略高于男性,但无显著差异。结论:CMIA检测血清VitB12的各项性能指标基本满足实验室要求,其可为实验室提供可靠的VitB12结果,为实验室评价人群VitB12营养状况提供信息。%Objective:To evaluate the performance of paramagnetic

  16. 化学发光免疫分析法检测抗 TP 抗体S/CO 比值与确证试验阳性的相关性研究%Study on the correlation between anti-TP antibody S/CO ratio by chemiluminescence immunoassay and positive results by confirmation assay

    Institute of Scientific and Technical Information of China (English)

    许晓峰; 江铭磊; 卢仁泉; 邹淳缘; 龚志赟; 沈利; 郭林

    2015-01-01

    Objective To investigate the correlation between anti-treponema pallidum(TP)antibody signal-to-cut off(S /CO)ratio by chemiluminescence immunoassay (CLIA)and positive results by confirmation assay.Methods A total of 208 anti-TP antibody positive samples (S /CO ratio≥1 .0)were screened out by 2 CLIA systems,Abbott i2000 CLIA system (Abbott i2000)and Kemei Dongya Chemclin 600 CLIA system (Chemclin 600 ).The results were confirmed by western blotting (WB).Receiver operating characteristic(ROC)curve at optimal sensitivity and specificity was analyzed,and optimal S /CO threshold value was obtained by SPSS 1 9.0 software.The positive predictive value was calculated with every S /CO ratio interval,and the S /CO ratio of positive predictive value≥95%was obtained.Results By Abbott i2000 or Chemclin 600,208 samples′S /CO ratio was determined.By WB,35 cases of them were confirmed as negative,1 28 cases were confirmed as positive,and 45 cases were indeterminate.The coincidence rates of WB compared with the 2 CLIA systems were 82.2% and 82.8%,respectively.When anti-TP antibody S /CO ratio of Abbott i2000 was ≥3.0,and anti-TP antibody S /CO ratio of Chemclin 600 was ≥4.0,the positive predictive value was≥95%.Conclusions It is proposed that WB should be used if the S /CO ratio ranges are 1 .0-3.0 determined by Abbott i2000 and 1 .0-4.0 determined by Chemclin 600.If the S /CO ratio is above the upper limit,the samples can be reported as positive directly.It can not only reduce the confirmation assay sample number but also improve the reliability of determination report.%目的:探讨化学发光免疫分析法(CLIA)检测抗梅毒螺旋体(TP)抗体筛查试验信号/临界值(S/CO)比值与确证试验阳性的相关性。方法经 Abbott i2000 CLIA 系统(简称 Abbott i2000)或科美东雅 Chemclin 600 CLIA 系统(简称 Chemclin 600)检测抗 TP 抗体 S /CO 比值≥1.0的血清样本208份,用免疫印迹法(WB)对抗 TP抗体

  17. Study on prenatal screening for birth deficiency in the pregnant metaphase by the serum AFP, β-HCG and uE3 with chemiluminescent immunoassay%联合检测孕中期血清甲胎蛋白、β-绒毛膜促性腺激素、游离雌三醇筛查出生缺陷的应用研究

    Institute of Scientific and Technical Information of China (English)

    邱振华; 高艳; 吴玉华; 戚子惠; 黄金波; 舒云华; 陈宇锋

    2009-01-01

    Objective To evaluate the effect of the serum markers of AFP, β-HCG and uE3 on screening of congenital fetal abnormality in the pregnant women at second trimester. Methods The serum markers were measured in 2 149 pregnant women at second trimester of gestation with chemiluminescent immunoassay, and were combined with correlation factors such as the age, gestational weeks and the weight of pregnant women, to estimate the probability of fetus suffering from Down's syndrome, open spina bifida and Trisomy 18 syndrome. Cell chromosomes and ultrasonography were also done in high risk patients. Analysis was carried out based on the results of follow-up survey. Results One hundred and thirty-six (6.33%) cases wind high risk of birth deficiency were found in preliminary screening from 2 149 pregnant women, in which the positive rates of Down's syndrome, open spina bifida and Trisomy 18 syndrome were 4.14%, 1.77% and 0.42%,respectively. The positive rates were 66.67% and 100% and the false positive rates were 3.75% and 1.85% in Down's syndrome and open spina bifida. Conclusion The combined screening of maternal serum markers of AFP, β-HCG and uE3 is reliable and effective, and is the important measure to reduce birth deficiencify combined with prenatal diagnosis.%目的:评价联合检测孕妇孕中期(14~19+6周)血清标志物甲胎蛋白、β-绒毛膜促性腺激素、游离雌三醇在产前筛查先天出生缺陷中的应用价值.方法:应用化学发光免疫分析技术检测2 149例孕妇孕中期血清标志物,并结合孕妇年龄、孕周、体质量等因素,应用仪器配套的产前筛查风险分析软件计算唐氏综合征、18-三体综合征和开放性脊柱裂的风险率,对高风险孕妇采用B超和/或细胞染色体检查进行确认,根据随访及诊断结果进行评估.结果:2 149例孕妇筛查出出生缺陷高风险136例,筛查阳性率为6.33%,其中唐氏综合征、开放性脊柱裂和18-三体综合征

  18. Exercício físico e estresse oxidativo: efeitos do exercício físico intenso sobre a quimioluminescência urinária e malondialdeído plasmático Ejercício físico y estres oxidativo: efectos del ejercício físico intenso sobre la quimioluminiscencia urinaria y el malondialdheído plasmático Physical exercise and oxidative stress: effect of intense physical exercise on the urinary chemiluminescence and plasmatic malondialdehyde

    Directory of Open Access Journals (Sweden)

    Tácito Pessoa de Souza Jr.

    2005-02-01

    exercise causes oxidative stress in animals and humans, being possibly related, for instance, to fatigue and tissue lesions. However, the effects of high intensity exercise or training performed by athletes on the occurrence of oxidative stress are not fully clear, possibly due to methodological limitations. The objective of this study was to identify the occurrence of oxidative lesions in lipids due to physical training in athletes, through the quantification of the urinary chemiluminescence and plasmatic malondialdehyde (MDA. Post-exercise samples were collected after four training protocols: a treadmill running (25-30 min; b 20 km running performed by marathon runners; c interval training accomplished by 400 m runners; d soccer game with 50 min duration; and e strength training with and without creatine supplementation. In the last four items, only the urinary chemiluminescence was evaluated. The conditions that presented elevation in urinary chemiluminescence after exercise completion were: a 20 km running; b soccer game; and c strength training without creatine supplementation. The treadmill running increased plasmatic MDA concentration during and after its performance, and the plasmatic antioxidant capacity had an inverse behavior compared to the increase in MDA. The exercise used in this work promoted oxidative stress in a different way and this may be related to the duration and the intensity performed by athletes, and not only to intensity. In this work it was also observed that creatine ingestion associated with strength training might work as antioxidant.

  19. Soot and chemiluminescence in diesel combustion of bio-derived, oxygenated and reference fuels

    NARCIS (Netherlands)

    Klein-Douwel, R. J. H.; Donkerbroek, A. J.; van Vliet, A. P.; Boot, M. D.; Somers, L. M. T.; Baert, R. S. G.; Dam, N. J.; ter Meulen, J. J.

    2009-01-01

    High-speed imaging, spectroscopy and thermodynamical characterization are applied to an optically accessible, heavy-duty diesel engine in order to compare sooting and chemilunlinescence behaviour of bio-derived, oxygenated fuels and various reference fuels. The fuels concerned include the bio-derive

  20. Electrogenerated chemiluminescence of luminol for oxidase-based fibre-optic biosensors.

    Science.gov (United States)

    Marquette, C A; Leca, B D; Blum, L J

    2001-01-01

    The luminol electrochemiluminescence has been exploited for the development of several fibre-optic biosensors allowing the detection of hydrogen peroxide and of substrates of H(2)O(2)-producing oxidases. Electro-optical flow injection analysis of glucose, lactate, cholesterol and choline are thus described. To perform the experiments, a glassy carbon electrode was polarized at a fixed potential. Luminol was then electrochemically oxidized and could react in the presence of hydrogen peroxide to produce light. Several parameters had to be optimized to obtain reliable optical biosensors. An optimum applied potential of +425 mV between the glassy carbon electrode and the platinum pseudo-reference electrode was determined, allowing the best signal: noise ratio to be obtained. It was also necessary to optimize the experimental conditions for the immobilization of the different oxidases involved (preactivated membranes, chemically activated collagen membranes, photopolymerized matrix). For each biosensor developed, the optimum reaction conditions have been studied: buffer composition, pH, temperature, flow rate and luminol concentration. Under optimal conditions, the detection limits (S/N = 3) were 30 pmol, 60 pmol, 0.6 nmol and 10 pmol for lactate, glucose, cholesterol and choline, respectively. The miniaturization of electrochemiluminescence-based biosensors has been realized using screen-printed electrodes instead of a glassy carbon macroelectrode, with choline oxidase as a model H(2)O(2)-generating oxidase. PMID:11312542

  1. DETERMINATION OF TETRACYCLINE AND OXYTETRACYCLINE BY FLOW-INJECTION CHEMILUMINESCENCE METHOD

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Tetracycline and oxytetracycline are broad-spec-trumantibiotics.They are not only used in humanpathologies,but alsoin veterinary medicine,ani malnutrition and feed additives for cattle breeding.In the past few years,the deter mination meth-ods of tetracycline and oxytetracycline were repor-ted,which involved in difference spectrophotome-try[1],HPLC[2,3],spectrophotometry[4-7],HPLC-MS[8-9],spectrofluori metry[10],solid-phase extrac-tion[11]and kinetic methods.Recently,flow-injection CL method has beenpaid mo...

  2. CHEMILUMINESCENT MEASUREMENTS OF NITRIC OXIDE PULMONARY DIFFUSING CAPACITY AND ALVEOLAR PRODUCTION IN HUMANS. (R826781)

    Science.gov (United States)

    The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

  3. A comparison of the presumptive luminol test for blood with four non-chemiluminescent forensic techniques.

    Science.gov (United States)

    Webb, Joanne L; Creamer, Jonathan I; Quickenden, Terence I

    2006-01-01

    Presumptive blood detection tests are used by forensic investigators to detect trace amounts of blood or to investigate suspicious stains. Through the years, a number of articles have been published on the popular techniques of the day. However, there is no single paper that critiques and compares the five most common presumptive blood detection tests currently in use: luminol, phenolphthalein (Kastle-Meyer), leucomalachite green, Hemastix and the forensic light source. The present authors aimed to compare the above techniques with regard to their sensitivity, ease of use and safety. The luminol test was determined to be the most sensitive of the techniques, while Hemastix is a suitable alternative when the luminol test is not appropriate.

  4. Competitive chemiluminescent anzyme immunoassay for vitamin B12 analysis in human milk.

    Science.gov (United States)

    Recent discoveries of matrix interferences by haptocorrin (HC) in human milk and serum show that past analyses of vitamin B12 in samples with high HC content might have been inaccurate (Lildballe et al., 2009; Carmel & Agrawal, 2012). We evaluated two competitive enzyme-binding immunoassays for seru...

  5. Spectral Analysis of Ultraweak Chemiluminescence from Kidney Bean Leaf Infested with Tetranychus Kanzawai Kishida

    Science.gov (United States)

    Kawabata, Ryuzou; Miike, Tohru; Okabe, Hirotaka; Uefune, Masayoshi; Takabayashi, Junji; Takagi, Masami; Kai, Shoichi

    2005-02-01

    We performed the spectral analysis of ultraweak-photon emissions from kidney bean leaves infested by the kanzawa spider mite, Tetranychus kanzawai Kishida (Acari: Tetranychidae). We also measured the spectrum of photon emissions from artificially wounded leaves, and compared the result with spectral data obtained from the mite-infested leaves. Photon emissions from both the mite-infested and wounded leaves primarily consisted of wavelengths ranging from 500 to 700 nm, and photon intensity at these wavelengths increased steadily after perturbation. In contrast, photon intensity of the mite-infested leaves at 300-400 nm exhibited only differential changes; it began increasing at 20 h, and showed two peaks at 72 and 120 h. We previously reported that photon emissions from infested leaves might be the result of both insect damage and plant self-protection. Plant defensive responses, such as herbivore-induced plant volatiles (HIPV), are induced by insect elicitors via insect damage. Therefore, photon intensity at 500-700 nm might be related to direct injury (physiological stress), while photon intensity at 300-400 nm may signify a physiological (biochemical)-action-related defensive response.

  6. Evaluation of the antioxidant effects of four main theaflavin derivatives through chemiluminescence and DNA damage analyses

    Institute of Scientific and Technical Information of China (English)

    Yuan-yuan WU; Wei LI; Yi XU; En-hui JIN; You-ying TU

    2011-01-01

    Theaflavins (TFs) are the dimers of a couple of epimerized catechins,which are specially formed during black tea fermentation.To explore the differences among four main TF derivatives (theaflavin (TF1),theaflavin3-gallate (TF2A),theaflavin-3′-gallate (TF2B),and theaflavin-3,3′-digallate (TF3)) in scavenging reactive oxygen species (ROS) in vitro,their properties of inhibiting superoxide,singlet oxygen,hydrogen peroxide,and the hydroxyl radical,and their effects on hydroxyl radical-induced DNA oxidative damage were systematically analyzed in the present study.The results show that,compared with (-)-epigallocatechin gallate (EGCG),TF derivatives were good antioxidants for scavenging ROS and preventing the hydroxyl radical-induced DNA damage in vitro.TF3 was the most positive in scavenging hydrogen peroxide and hydroxyl radical,and TF1 suppressed superoxide.Positive antioxidant capacities of TF2B on singlet oxygen,hydrogen peroxide,hydroxyl radical,and the hydroxyl radical-induced DNA damage in vitro were found.The differences between the antioxidant capacities of four main TF derivatives in relation to their chemical structures were also discussed.We suggest that these activity differences among TF derivatives would be beneficial to scavenge different ROS with therapeutic potential.

  7. Anodic electrogenerated chemiluminescence behavior and the choline biosensing application of blue emitting conjugated polymer dots.

    Science.gov (United States)

    Chen, Hongmei; Lu, Qiyi; Liao, Jiayao; Yuan, Ruo; Chen, Shihong

    2016-06-01

    The anodic electrochemiluminescence (ECL) behavior of poly(9,9-dioctylfluorenyl-2,7-diyl) (PFO) dots was studied and applied in oxidoreductase-based ECL biosensing using Chox as the model enzyme. PMID:27181622

  8. A Novel Flow Injection Chemiluminescence System for the Determination of Reserpine

    Institute of Scientific and Technical Information of China (English)

    ZHUANG Hui-sheng; WANG Qiong-e; WEI Qiao-hua; WU Yan-lin

    2004-01-01

    A novel flow injection cheminescence (FICL) system for the determination of reserpine is presented in this paper. It is based on the CL reaction of reserpine, IO-4 and H2O2 in H2SO4 medium. The CL intensity was correlated with concentration of reserpine in the range of 1.0×10-6~1.0×10-4g/mL with a detection limit of 1.35×10-7g/ mL. The relative standard deviation is 2.63% for 11 measurements of 2×10-5g/mL reserpine standard solution. The method has been applied to the determination of reserpine in pharmaceutical preparations and the results are in good agreement with that of the standard method.

  9. A Wireless Chemiluminesce Detector for In-Situ Monitoring for AFEC Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Pentalim Inc. is developing a new sensor for the measurement of chemiluninescence of air breathing engine combustion. The sensor will be wireless and incorporate...

  10. The effect of dietary fish oil-supplementation to healthy young men on oxidative burst measured by whole blood chemiluminescence

    DEFF Research Database (Denmark)

    Bartelt, Stine; Timm, Michael; Damsgaard, Camilla Trab;

    2008-01-01

    a randomised 2 £ 2-factorial design in which subjects were randomly assigned to 8-week supplementation with capsules containing fish oil (about 29 g n-3 LCPUFA/d) or olive oil (control). Subjects were also randomly assigned to household use of oils and fat spreads with a high or a low 18 : 2n-6 content...... by the fish oil-supplementation (P,0001, compared to the olive oil groups). No effect of the intervention was observed on neutrophil count, but one measure of the zymosan-induced oxidative burst was higher in the fish oil groups (P¼003) compared to the olive oil groups. The fat intervention did not in itself...

  11. TSH BAS-CLIA方法的建立%Chemiluminescent Immunoassay for TSH Using Biotin-streptavidin System

    Institute of Scientific and Technical Information of China (English)

    尹东光; 贺佑丰; 刘一兵; 沈德存; 韩世泉; 罗志福

    2003-01-01

    吖啶酯DMAE*NHS标记链亲和素, 用生物素标记一株TSH单克隆抗体, 另一株TSH单抗包被96微孔板, 建立TSH BAS-CLIA方法. 该方法标准曲线的线性相关系数为0.999,分析灵敏度为0.007mIU/L.批内变异系数为2.36%-3.66%, 批间变异系数为2.44%-4.35%, 平均回收率为101.7%.与TSH IRMA法的回归方程为: Y=-0.015+1.02X, 相关系数为0.989; 与本文的TSH CLIA也有明显相关, 相关方程为: Y=-0.24+1.01X, 相关系数为0.992;与Ciba Corining TSH CLIA临床测量值也呈明显相关.

  12. Enhanced chemiluminescence-based detection on gold substrate after electrografting of diazonium precursor-coated gold nanoparticles.

    Science.gov (United States)

    Houmed Adabo, Ali; Zeggari, Rabah; Mohamed Saïd, Nasser; Bazzi, Rana; Elie-Caille, Céline; Marquette, Christophe; Martini, Matteo; Tillement, Olivier; Perriat, Pascal; Chaix, Carole; Boireau, Wilfrid; Roux, Stéphane

    2016-04-01

    Since it was demonstrated that nanostructured surfaces are more efficient for the detection based on the specific capture of analytes, there is a real need to develop strategies for grafting nanoparticles onto flat surfaces. Among the different routes for the functionalization of a surface, the reduction of diazonium salts appears very attractive for the covalent immobilization of nanoparticles because this method does not require a pre-treatment of the surface. For achieving this goal, gold nanoparticles coated by precursor of diazonium salts were synthesized by reduction of gold salt in presence of mercaptoaniline. These mercaptoaniline-coated gold nanoparticles (Au@MA) were successfully immobilized onto various conducting substrates (indium tin oxide (ITO), glassy carbon (GC) and gold electrodes with flat terraces) after addition of sodium nitrite at fixed potential. When applied onto the gold electrodes, such a grafting strategy led to an obvious enhancement of the luminescence of luminol used for the biodetection. PMID:26803605

  13. CS(a/sup 3/. pi. /sub r/) chemiluminescence from the reaction C+OCS at 300 K

    Energy Technology Data Exchange (ETDEWEB)

    Dorthe, G.; Caille, J.; Burdenski, S.

    1983-01-01

    The reaction C+OCS is proposed as a new candidate for an electronic transition chemical laser. A strong chemilumenescence is observed from CS between 340 and 390 nm. The characteristic of this emission is futher discussed. (AIP)

  14. CHEMILUMINESCENT MEASUREMENTS OF NITRIC OXIDE PULMONARY DIFFUSING CAPACITY AND ALVEOLAR PRODUCTION IN HUMANS. (R827354C003)

    Science.gov (United States)

    The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

  15. Synthesis, Photophysics, Electrochemistry and Electrogenerated Chemiluminescence of PEG-Modified BODIPY dyes in Organic and Aqueous Solutions

    Science.gov (United States)

    Nepomnyashchii, Alexander B.; Pistner, Allen J.; Bard, Allen J.; Rosenthal, Joel

    2013-01-01

    A set polyethylene glycol (PEG) appended BODIPY architectures (BOPEG1 – BOPEG3) have been prepared and studied in CH2Cl2, H2O:CH3CN (1:1) and aqueous solutions. BOPEG1 and BOPEG2 both contain a short PEG chain and differ in substitution about the BODIPY framework. BOPEG3 is comprised of a fully substituted BODIPY moiety linked to a PEG polymer that is roughly 13 units in length. The photophysics and electrochemical properties of these compounds have been thoroughly characterized in CH2Cl2 and aqueous CH3CN solutions. The behavior of BOPEG1 – BOPEG3 correlates with established rules of BODIPY stability based on substitution about the BODIPY moiety. ECL for each of these compounds was also monitored. BOPEG1, which is unsubstituted at the 2- and 6-positions dimerized upon electrochemical oxidation while BOPEG2, which contains ethyl groups at the 2- and 6-positions, was much more robust and served as an excellent ECL luminophore. BOPEG3 is highly soluble in water due to the long PEG tether and demonstrated modest ECL activity in aqueous solutions using tri-n-propylamine (TPrA) as a coreactant. As such, BOPEG3 represents the first BODIPY derivative that has been shown to display ECL in water without the need for an organic cosolvent, and marks an important step in the development of BODIPY based ECL probes for various biosensing applications. PMID:23626863

  16. Repeated spurious elevation of serum prostate-specific antigen values solved by chemiluminescence analysis: A possible interference by heterophilic antibodies

    Science.gov (United States)

    Bayó, Miquel; Muñoz-Rodríguez, Jesús; Bellido, Jose Antonio; Abascal-Junquera, Jose María; Hannaoui, Naim; Banús, Josep Maria

    2015-01-01

    Heterophilic antibodies are human immunoglobulins directed against various animal antigens. They can produce false-positive results in the analysis of different tumor markers, including prostate-specific antigen. This interference can lead to misdiagnosis, unnecessary tests, and overtreatment in some cases. We present herein the case of a 52-year-old man with repeated spurious elevation of prostate-specific antigen, reaching levels of 108.7 ng/mL, that were suspected to be caused by heterophilic antibodies. The interference was solved by changing the analysis technique. Real values of prostate-specific antigen were less than 1 ng/mL. PMID:26568798

  17. Rapid screening test for primary hyperaldosteronism: ratio of plasma aldosterone to renin concentration determined by fully automated chemiluminescence immunoassays.

    NARCIS (Netherlands)

    Perschel, F.H.; Schemer, R.; Seiler, L.; Reincke, M.; Deinum, J.; Maser-Gluth, C.; Mechelhoff, D.; Tauber, R.; Diederich, S.

    2004-01-01

    BACKGROUND: The ratio of plasma aldosterone concentration to plasma renin activity (PAC/PRA) is the most common screening test for primary hyperaldosteronism (PHA), but it is not standardized among laboratories. We evaluated new automated assays for the simultaneous measurement of PAC and plasma ren

  18. Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads

    Directory of Open Access Journals (Sweden)

    Zuhong Lu

    2006-08-01

    Full Text Available Superparamagnetic polymers (SPMP microbead was an excellent form fornanosized magnetic particles less than 10nm to realize their potential applications in manyfields. We developed a novel modified suspension polymerization method for the productionof superparamagnetic poly (methacrylate divinylbenzene (PMA-DVB microbeads, withamino groups on their surface after simple modifications. We applied these SPMPmicrobeads to establish a sandwich enzyme chemiluminence immuno (ECLIA procedure ofdetecting the free hCGβ in serum. It was proved to a better method compared to the ELISA,since it need half of the sample volume, simpler protocols, and the time it need wasshortened from 2 hours to 1 hour. The detection limit was 0.22 mIU•m-1, one order lowerthan the ELISA assay. Its linear range was between 0.45—185.2 mIU•m-1. And in thedetections of clinical serum samples, the related coefficient between the two methods’results was 0.955.

  19. Enhanced chemiluminescence of CdTe quantum dots-H₂O₂ by horseradish peroxidase-mimicking DNAzyme.

    Science.gov (United States)

    Zhang, Junli; Li, Baoxin

    2014-05-01

    In this study, it was found that horseradish peroxidase (HRP)-mimicking DNAzyme could effectively enhance the CL emission of CdTe quantum dots (QDs)-H2O2 system, whereas HRP could not enhance the CL intensity. The CL enhancement mechanism was investigated, and the CL enhancement was supposed to originate from the catalysis of HRP-mimicking DNAzyme on the CL reaction between CdTe QDs and H2O2. Meantime, compared with CdTe QDs-H2O2 CL system, H2O2 concentration was markedly decreased in QDs-H2O2-HRP-mimicking DNAzyme CL system, improving the stability of QDs-H2O2 CL system. The QDs-based CL system was used to detect sensitively CdTe QDs and HRP-mimicking DNAzyme (as biologic labels). This work gives a path for enhancing CL efficiency of QDs system, and will be helpful to promote the step of QDs application in various fields such as bioassay and trace detection of analyte. PMID:24556131

  20. Distribution of energy in bimolecular chemiluminescent reactions involving hydrogen atoms. Final report, May 1, 1978-April 30, 1979

    International Nuclear Information System (INIS)

    Analysis of spectroscopic observations of the production of visible and ultraviolet photons from the collision of approx. 5-eV H atoms with a Li/Li2 crossed beam and with 02 and H2O crossed beams is described. Complementary theoretical and nonbeam spectroscopic work related to the Li2H system is also noted. 5 figures, 1 table

  1. Assessment of Antioxidant Activity of Spray Dried Extracts of Psidium guajava Leaves by DPPH and Chemiluminescence Inhibition in Human Neutrophils

    OpenAIRE

    2014-01-01

    This work evaluated the physicochemical properties and antioxidant activity of spray dried extracts (SDE) from Psidium guajava L. leaves. Different drying carriers, namely, maltodextrin, colloidal silicon dioxide, Arabic gum, and β -cyclodextrin at concentrations of 40 and 80% relative to solids content, were added to drying composition. SDE were characterized through determination of the total phenolic, tannins, and flavonoid content. Antioxidant potential of the SDE was assessed by two assa...

  2. The study of chemiluminescence in gastric and colonic carcinoma cell lines treated by anti-tumor drugs

    Institute of Scientific and Technical Information of China (English)

    Che Chen; Fu-Kun Liu; Xiao-Ping Qi; Jie-Shou Li

    2003-01-01

    AIM: To study the influence of chemotherapy on proliferationactivation of tumor cell by observing the change ofchemiluminescence (CL) and cell cycle in various tumor celllines after mitomycin C treated.METHODS: BGC823 and LoVocell lines were all cultured inRPMI-1640, and then were adjusted to a concentration of1x105 cells/ml in fresh media.and incubated for 24 h.Mitomycin C (100 ng@ml-1) was added to each bottle. Allindeses were examined after 24 h. No Mitomycin C wasadded in control group. Each group contained 8 samples.Flow cytometric analysis and luminol-dependent CL wereused to investigate the effect of mitomycin C on twogastrointestinal carcinoma cell lines.RESULTS: BGC823 and LoVo cell lines incubated with MMCfor 24 h. We discovered that the emergence of peak of CLstimulated by PHA was postponed significantly (BGC823:12.63±3.21 vs 4.50±1.04, LoVo: 13.25+2.96 vs 5.12±1.36,P<0.01) and the peak intension of CL was reduced significantly(BGC823:120.25±16.61 vs248.38±29.17, LoVo: 98.13±10.49vs267.50±18.56, P<0.01).The PI of cell lines was decreasedsignificantly (BGC823:51.87±4.82 vs 25.44±2.26, LoVo:47.11±1.04 vs 24.23±0.37, P<0.01) and the apoptoticfractions changed by contraries (BGC823:26.25+5.29 vs9.83±2.51, LoVo: 33.50±3.68 vs9.63±1.44, P<0.01).CONCLUSION: CL can be used to measure activation oftumor cells. We discovered that the ground CL intensions oftwo cell lines were not high but increased rapidly afterstimulation of PHA. The CL peak ranged from 4-5 minutes,and then decreased gradually. The results were not reportedbefore. CL of tumor cell has close correlativity with thedynamics of cell cycle and can reflect the feature of oxidationmetabolism and proliferation activation of tumor cell. So itcan be used to observe the influence of chemotherapydrug on metabolism and proliferation activation of tumorcell and screen out chemotherapy drugs to which tumorcells are sensitive.

  3. Utilization of the human cell line HL-60 for chemiluminescence based detection of microorganisms and related substances

    DEFF Research Database (Denmark)

    Timm, Michael; Hansen, Erik W; Moesby, Lise;

    2006-01-01

    In this paper we describe a new pyrogen assay using the human leukemia cell line HL-60. The cell line is differentiated using all-trans retinoic acid (ATRA) to generate a cell population that resembles mature granulocytes. The differentiated HL-60 cell is capable of generating reactive oxygen...... species (ROS) when challenged with pyrogenic substances. In a luminol enhanced chemilumimetric assay the responsiveness of differentiated HL-60 cells is tested towards Salmonella typhimurium, Bacillus subtilis, Saccharomyces cerevisiae, Candida albicans, lipopolysaccharide (LPS) and lipoteichoic acid (LTA......). The results show a poor sensitivity to S. typhimurium but displays good sensitivity towards B. subtilis, LTA and LPS. Furthermore, the sensitivity towards the yeasts C. albicans and S. cerevisiae is considerably better than obtained in other in vitro cell systems. Overall these results indicate that the HL-60...

  4. Rapid and simultaneous detection of ricin, staphylococcal enterotoxin B and saxitoxin by chemiluminescence-based microarray immunoassay

    OpenAIRE

    Szkola, A.; Linares, E. M.; Worbs, Sylvia; Dorner, Brigitte; R. Dietrich; Märtlbauer, E.; Niessner, R.; Seidel, Michael

    2014-01-01

    Simultaneous detection of small and large molecules on microarray immunoassays is a challenge that limits some applications in multiplex analysis. This is the case for biosecurity, where fast, cheap and reliable simultaneous detection of proteotoxins and small toxins is needed. Two highly relevant proteotoxins, ricin (60 kDa) and bacterial toxin staphylococcal enterotoxin B (SEB, 30 kDa) and the small phycotoxin saxitoxin (STX, 0.3 kDa) are potential biological warfare agents and require an a...

  5. LIQUID-CHROMATOGRAPHIC ANALYSIS OF CARBOXYLIC-ACIDS USING N-(4-AMINOBUTYL)-N-ETHYLISOLUMINOL AS CHEMILUMINESCENT LABEL - DETERMINATION OF IBUPROFEN IN SALIVA

    NARCIS (Netherlands)

    STEIJGER, OM; LINGEMAN, H; BRINKMAN, UAT; HOLTHUIS, JJM; SMILDE, AK; DOORNBOS, DA

    1993-01-01

    N-(4-Aminobutyl)-N-ethylisoluminol was used for labelling of carboxylic acids. The derivatization reaction was carried out with 1-hydroxybenzotriazole as pre-activator of the carboxylic acid function and N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide as the coupling reagent. Optimum conditions for t

  6. Antioxidant activities of two sericin proteins extracted from cocoon of silkworm (Bombyx mori) measured by DPPH, chemiluminescence, ORAC and ESR methods

    OpenAIRE

    TAKECHI, TAYORI; WADA, RITSUKO; FUKUDA, TSUBASA; HARADA, KAZUKI; TAKAMURA, HITOSHI

    2014-01-01

    Recent efforts have focused on the use of sericin proteins extracted from cocoons of silkworm as a healthy food source for human consumption. In this study, we focused on the antioxidative properties of sericin proteins. The antioxidative properties were measured in sericin proteins extracted from the shell of the cocoon, designated hereafter as white sericin protein and yellow-green sericin protein, as well as bread without sericin protein and bread to which white sericin powder had been add...

  7. Chemiluminescence analysis of the effect of butanol-diesel fuel blends on the spray-combustion process in an experimental common rail diesel engine

    Directory of Open Access Journals (Sweden)

    Merola Simona Silvia S.

    2015-01-01

    Full Text Available Combustion process was studied from the injection until the late combustion phase in an high swirl optically accessible combustion bowl connected to a single cylinder 2-stroke high pressure common rail compression ignition engine. Commercial diesel and blends of diesel and n-butanol (20%: BU20 and 40%: BU40 were used for the experiments. A pilot plus main injection strategy was investigated fixing the injection pressure and fuel mass injected per stroke. Two main injection timings and different pilot-main dwell times were explored achieving for any strategy a mixing controlled combustion. Advancing the main injection start, an increase in net engine working cycle (>40% together with a strong smoke number decrease (>80% and NOx concentration increase (@50% were measured for all pilot injection timings. Compared to diesel fuel, butanol induced a decrease in soot emission and an increase in net engine working area when butanol ratio increased in the blend. A noticeable increase in NOx was detected at the exhaust for BU40 with a slight effect of the dwell-time. Spectroscopic investigations confirmed the delayed auto-ignition (~60 ms of the pilot injection for BU40 compared to diesel. The spectral features for the different fuels were comparable at the start of combustion process, but they evolved in different ways. Broadband signal caused by soot emission, was lower for BU40 than diesel. Different balance of the bands at 309 and 282 nm, due to different OH transitions, were detected between the two fuels. The ratio of these intensities was used to follow flame temperature evolution.

  8. Evaluation of a new chemiluminescence technique for human thyrotropin (BeriLux hTSH): diagnostic value of five immunometric assay methods.

    Science.gov (United States)

    Hashimoto, T; Matsubara, F; Nishibu, M; Kawai, K

    1991-11-01

    A new commercially available human thyrotropin immunochemiluminometric assay (ICMA) kit was evaluated. The BeriLux assay (Hoechst Co., Germany) was compared with two other non-radioisotopic methods (AIA-1200 and IMx) and two other immunoradiometric assays (RIA-gnost TSH IRMA and EIKEN IRMA kits) in 32 normal subjects and 104 patients with Graves' disease, divided into seven groups: 1) untreated hyperthyroidism; 2) hyperthyroidism during treatment; 3) euthyroid with negative thyroliberin test (subclinical hyperthyroidism); 4) euthyroid with low thyroliberin test; 5) euthyroid with normal thyroliberin test; 6) euthyroid with high thyrotropin level (subclinical hypothyroidism); and 7) primary hypothyroidism. Patients in groups 2-6 were undergoing treatment with mercazole and propylthiouracil. The new immunoluminometric assay (ILMA) BeriLux kit was shown to have a remarkably improved analytical and clinical sensitivity. The minimal detectable level of thyrotropin in the assay was 0.006 mU/l. The precision was 2.8% and 6.1% at 0.093 +/- 0.003 mU/l and 0.028 +/- 0.002 mU/l, respectively, whereas the precision of the other methods was above 17.2% and 59.4% respectively. Seven patients from the untreated hyperthyroid group were given 500 micrograms thyroliberin i.v. (the thyroliberin test). The thyrotropin pattern before and after thyroliberin administration was always less than 0.006 mU/l with the BeriLux kit, whereas the other methods showed random fluctuations indicating their low accuracy at this concentration. Using the BeriLux kit, 7 of the 16 overt hyperthyroid patients undergoing treatment showed a measurable thyrotropin level below 0.01 mU/l but a negative thyroliberin test.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:1782283

  9. Flow injection chemiluminescence method for the determination of cefalexin%流动注射化学发光法检测头孢氨苄

    Institute of Scientific and Technical Information of China (English)

    陈麒; 唐玉海; 孙媛媛; 姚宏

    2004-01-01

    目的利用头孢氨苄对鲁米诺-高碘酸钾化学发光反应有增敏作用,建立微量快速测定头孢氨苄流动注射化学发光分析法.方法用鲁米诺与高碘酸钾溶液在线混合为载液,头孢氨苄通过八通阀与载液混合后产生化学发光.结果检出限为9×10-10 g·ml-1;线性范围为1×10-9g·ml-1~2×10-7g·ml-1,相对标准偏差小于2%.结论此法用于头孢氨苄胶囊中药物含量的测定,结果较满意.

  10. Flow-Injection Chemiluminescence Determination of Cefalexin%流动注射化学发光法测定头孢氨苄

    Institute of Scientific and Technical Information of China (English)

    李永新; 朱英贵; 赵丹华; 卓淑娟; 朱昌青; 王伦

    2002-01-01

    基于头孢氨苄(CEX)降解产物中有还原性的巯基(SH)基团,在0.2 mol/L 的 H2SO4介质中,能与Ce (Ⅳ)产生化学发光反应,罗丹明6G可敏化其发光强度,结合流动注射技术,考察了本体系的化学发光条件,建立了一种测定头孢氨苄的化学发光新方法.该法线性范围为0.05-10.0 μg/mL,相关系数r = 0.9984,检出限为0.02 μg/mL,对1.0 μg/mL的头孢氨苄标准溶液进行连续9次平行测定,相对标准偏差为1.4%.

  11. Environmental effects on the production of Shiga-like toxins by Escherichia coli O157:H7 as revealed by sandwiched immuno-chemiluminescence detection

    Science.gov (United States)

    Tu, Shu-I.; Uknalis, Joseph; He, Yiping

    2009-05-01

    We have developed a sandwiched immuno assay to detect sensitively Shiga-like toxins (SLTs) produced by Escherichia coli O157:H7. The method involved the capture of toxins by specific immuno magnetic beads followed by tagging the toxins with peroxidase-labeled anti E. coli O157:H7 antibody. Upon addition of proper substrate, peroxidase induced luminescence was used to measure the presence of SLTs. We have previously demonstrated that co-incubation of shiga toxin (SLT) producing E. coli O157:H7 with certain other bacteria can inhibit toxin production but does not affect the growth of the E. coli. We show here that media in which the cells have grown been centrifuged from (conditioned media) have similar effects on cell growth and SLT production. Adjusting the pH and adding nutrients to the conditioned media did not have any effect on the reduction of SLT produced. Bacteria communicate with each other via secreted sensing molecules. Several types of the molecules have been identified. However, the mechanisms of control remain to be established. This pattern for bacteria growth and toxin production is also observed when quorum-sensing molecules of homoserine lactone and indole are added to the media prior to inoculation.

  12. Flow-injection Chemiluminescence Sensor for the Determination of Gallic Acid by Immobilizing Luminol and Periodate on Anion-exchange Resin

    Institute of Scientific and Technical Information of China (English)

    ZHANG,Si-Chun(张四纯); ZHOU,Guo-Jun(周国俊); JU,Huang-Xian(鞠熀先)

    2002-01-01

    A novel chemihuminescence (CL) sensor for the determination of gallic acid combined with flow injection analysis was developed by electrostatically immobilizing luminol and periodate on anion-exchange resins respectively. Gallic acid was sensed by its enhancing effect on the weak CL reaction between luminol and periodate, which were eluted from the ion exchang ecolumn.The possible reaction mechanism of the CL system was suggested and discussed. The response of the sensor to gallic acid concentration was linear over the range of 8.0 × 10-9-1.0 × 10-6 mol/L with a detection limit of 6.5 × 10-9 mol/L (3σσ). The relative standard deviation (RSD) for 7 repetitive determinations of gallic acid (1.0 × 10-7 moL/L) was 1.8%. The sensor could be used for over 400 times determination with a good reproducibility.

  13. Performance Evaluation of Abbott Chemiluminescence Immunoassay Kit for Assaying SCCA%化学发光法定量检测SCCA试剂盒性能评估

    Institute of Scientific and Technical Information of China (English)

    田峰; 齐素文

    2013-01-01

    目的 评估Abbott公司化学发光法定量检测鳞状细胞癌相关抗原(SCCA)试剂盒的临床性能.方法 对SCCA试剂盒的精密度、灵敏度、准确度、特异度、线性范围和方法学对比分别进行评估;取290名健康人群样本检测血清SCCA水平,统计分析并建立该实验室的参考范围.结果 SCCA试剂盒的批内变异系数(CV)分别为1.36 ng/ml(3.51%),10.64 ng/ml(2.93%)和46.52 ng/ml(2.65%),总CV分别为1.28 ng/ml(5.26%),11.04 ng/ml(4.75%)和46.61 ng/ml(4.29%);分析灵敏度为0.04 ng/ml;回收率为96%~106%;SCCA与CEA,CA199和CYFRA21-1的交叉反应率小于3%;线性范围上限达到68.56 ng/ml.与现有的其他商品化试剂进行比对,相关系数为0.987 7.正常参考范围为0.15~1.63 ng/ml.结论 Abbott化学发光法SCCA试剂盒性能优良,测试结果准确,为临床检测SCCA水平提供了一种新的选择.

  14. Electrogenerated Chemiluminescence Behavior of Au nanoparticles-hybridized Pb (II) metal-organic framework and its application in selective sensing hexavalent chromium

    OpenAIRE

    Hongmin Ma; Xiaojian Li; Tao Yan; Yan Li; Haiyang Liu; Yong Zhang; Dan Wu; Bin Du; Qin Wei

    2016-01-01

    In this work, a novel electrochemiluminescence (ECL) sensor based on Au nanoparticles-hybridized Pb (II)-β-cyclodextrin (Pb-β-CD) metal-organic framework for detecting hexavalent chromium (Cr(VI)) was developed. Pb-β-CD shows excellent ECL behavior and unexpected reducing ability towards Au ions. Au nanoparticles could massively form on the surface of Pb-β-CD (Au@Pb-β-CD) without use of any additional reducing agent. In the presence of coreactant K2S2O8, the ECL emission of Pb-β-CD was enhanc...

  15. Electrogenerated Chemiluminescence Behavior of Au nanoparticles-hybridized Pb (II) metal-organic framework and its application in selective sensing hexavalent chromium.

    Science.gov (United States)

    Ma, Hongmin; Li, Xiaojian; Yan, Tao; Li, Yan; Liu, Haiyang; Zhang, Yong; Wu, Dan; Du, Bin; Wei, Qin

    2016-01-01

    In this work, a novel electrochemiluminescence (ECL) sensor based on Au nanoparticles-hybridized Pb (II)-β-cyclodextrin (Pb-β-CD) metal-organic framework for detecting hexavalent chromium (Cr(VI)) was developed. Pb-β-CD shows excellent ECL behavior and unexpected reducing ability towards Au ions. Au nanoparticles could massively form on the surface of Pb-β-CD (Au@Pb-β-CD) without use of any additional reducing agent. In the presence of coreactant K2S2O8, the ECL emission of Pb-β-CD was enhanced by the formation of Au nanoparticles. Cr(VI) can collisionally quench the ECL behavior of Au@Pb-β-CD/S2O8(2-) system and the detection mechanism was investigated. This ECL sensor is found to have a linear response in the range of 0.01-100 μM and a low detection limit of 3.43 nM (S/N = 3) under the optimal conditions. These results suggest that metal-organic framework Au@Pb-β-CD has great potential in extending the application in the ECL field as an efficient luminophore. PMID:26902375

  16. Determination of Vitamin B6 by Flow Injection Chemiluminescence Method%流动注射化学发光测定维生素B6

    Institute of Scientific and Technical Information of China (English)

    陈小利; 马红燕; 吕俊芳; 张琰图

    2003-01-01

    基于维生素B6在碱性条件下对Luminol和KIO4的化学发光反应有很强的增敏作用,结合反向流动注射技术,建立了流动注射化学发光测定维生素B6的新方法.在优化条件下,维生素B6浓度在5.0×10-8~1.0×10-5 g/mL范围内,与化学发光强度呈良好的线性关系,方法的检出限为1.3×10-8 g/mL,对5.0×10-6 g/mL维生素B6进行11次平行测定,方法的相对标准偏差为1.5%.该方法成功用于药剂中维生素B6含量的测定.

  17. On Line Galvanic Cell Generated Chemiluminescence%自发电池激发的电化学发光分析

    Institute of Scientific and Technical Information of China (English)

    罗丽荣; 章竹君

    2008-01-01

    本文简单介绍了电化学发光分析近年来的研究进展.目前,文献报道的ECL分析都是使用外加的电源来实现ECL的激发,这就限制了ECL检测系统的微型化,及其在微全分析系统中的进一步应用.我们曾经设计了微型自发原电池为鲁米诺体系及钙黄绿素的电化学发光提供激发电位,大大地简化了ECL的设备,在ECL系统的微型化方面,迈出了新的步伐.

  18. Quantitation of Sodium Sulphite in Injection Liquid by Chemiluminescence Method%针剂注射液中亚硫酸钠含量的化学发光测定

    Institute of Scientific and Technical Information of China (English)

    袁华; 于化江; 杨凤珍; 牟金玲

    2009-01-01

    亚硫酸钠与酸性的吐温80,罗丹明6G混合溶液相遇产生强烈的光信号,其化学发光强度与亚硫酸钠的浓度在O.05-10μg mL-1(r=0.998)范围内呈良好的线性关系,方法的检出限为0.03μg mL-1.并成功应用于针剂注射液中Na2SO3含量的测定.

  19. 人附睾蛋白4化学发光定量测定法的建立与评价%Establishment and evaluation of a chemiluminescence quantitative immunoassay for detecting human epididymis protein 4

    Institute of Scientific and Technical Information of China (English)

    罗湘宇; 刘宇思; 曾令斌; 赵家宁; 何敏; 杨卫平; 尹一兵; 胥文春

    2014-01-01

    目的 建立人附睾蛋白4(HE4)的化学发光定量检测方法,并进行方法学评价.方法 血清中HE4与包被抗HE4单克隆抗体的微磁珠和辣根过氧化物酶(HRP)标记的抗HE4单克隆抗体反应形成双抗体夹心结构,HRP可催化发光底物液(含鲁米诺-H2O2-增强剂)产生大量光信号,根据光信号的量计算血清中HE4的浓度.依据国家体外诊断试剂性能评价指南性文件和美国临床和实验室标准化协会(CLSI)指南性文件,对本方法进行系统评价.结果 建立的方法空白限(LoB)为1.014pmol/L、检测限(LoD)为5.252 pmol/L、定量检测限(LoQ)为10.568 pmol/L;甲胎蛋白(α-fetoprotein,AFP)≤400 IU/mL、癌胚抗原(carcinoembryonic antigen,CEA)≤1 777 μg/L、糖类抗原(carbohydrate antigen,CA)125≤3 500 U/mL、CA19-9≤3 500U/mL对本方法无明显交叉反应;线性范围为20~1 700pmol/L;在100 000 pmol/L时未出现明显钩状(Hook)效应;批内变异系数(CV)为1.5% ~3.6%;日间CV为3.8%~6.4%;回收率为98.36%~99.14%;血红蛋白≤4.8 g/L、胆红素≤1 077.5μmol/L、乳糜微粒≤6000浊度、生物素≤50 μg/L、类风湿因子≤1000U/L对测定结果无明显影响;37℃保存7d后相对偏差为-6.63%~10.23%;建立的方法(y)与Fujirebio公司的ELISA试剂(X)进行方法学比对,相关曲线为Y=1.023X-12.280,r=0.989 7(P<0.01).结论 本研究建立的HE4磁微粒化学发光免疫测定法各项性能符合临床实验室需求,为卵巢癌的辅助诊断提供了有效工具.

  20. Study on Antioxidation of Beet Red Pigment by Fluorescence and Chemiluminescence%采用荧光化学发光法研究甜菜红色素的抗氧化作用

    Institute of Scientific and Technical Information of China (English)

    吕晓玲; 孔德莉; 姚秀玲; 张佳宜

    2006-01-01

    用精制后生产出的甜菜红色素粉末进行抗氧化性研究,用荧光化学发光法测定了色素对活性氧自由基(ROS)的清除作用.实验中,采用Vc作对照,结果表明,甜菜红色素能显著清除机体外产生的超氧阴离子(O2-·)、羟自由基(·OH)和过氧化氢(H2O2),并呈现量效关系.