WorldWideScience

Sample records for chalones

  1. Chalon/Saint-Marcel manufacturing plant

    2008-01-01

    AREVA is the world leader in the design and construction of nuclear power plants, the manufacture of heavy components, and the supply of nuclear fuel and nuclear services such as maintenance and inspection. The Equipment Division provides the widest range of nuclear components and equipment, manufactured at its two facilities in Jeumont, northern France, and St. Marcel, in Burgundy. The St. Marcel plant, set on 35 ha (87.5 acres) near Chalon-sur-Saone, was established in 1973 in a region with a long history of specialized metalworking and mechanical activities to meet the demand for non-military nuclear requirements in France. The site offers two advantages: - excellent facilities for loading and transporting heavy components on the Saone river, - it's proximity to other group sites. Since its completion in 1975, the Chalon/St. Marcel facility has manufactured all the heavy components for French pressurized water reactors (PWRs) ranging from 900 MW to 1500 MW. It has also completed a significant number of export contracts that have made AREVA world leader. Nearly 600 heavy components (reactor vessels, steam generators, pressurizers and closure heads) have been manufactured or are currently being manufactured since the plant opened in 1975. The plant is at the heart of the manufacturing chain for nuclear steam supply systems (NSSS) supplied by AREVA. On the basis of engineering data, the plant manufactures reactor vessels, reactor vessel internals, steam generators, pressurizers and related components such as accumulators, auxiliary heat exchangers and supporting elements. Vessel upper internals Other similar components such as reactor vessels for boiling water reactors (BWR) or high temperature reactors (HTR) and other types of steam generators can also be manufactured in the plant (for example Once Through Steam Generators - OTSG). The basic activities performed at Chalon/St. Marcel are metalworking and heavy machining. These activities are carried out in strict

  2. Chalon/Saint-Marcel manufacturing plant; L'usine de Chalon/Saint-Marcel

    NONE

    2008-07-01

    AREVA is the world leader in the design and construction of nuclear power plants, the manufacture of heavy components, and the supply of nuclear fuel and nuclear services such as maintenance and inspection. The Equipment Division provides the widest range of nuclear components and equipment, manufactured at its two facilities in Jeumont, northern France, and St. Marcel, in Burgundy. The St. Marcel plant, set on 35 ha (87.5 acres) near Chalon-sur-Saone, was established in 1973 in a region with a long history of specialized metalworking and mechanical activities to meet the demand for non-military nuclear requirements in France. The site offers two advantages: - excellent facilities for loading and transporting heavy components on the Saone river, - it's proximity to other group sites. Since its completion in 1975, the Chalon/St. Marcel facility has manufactured all the heavy components for French pressurized water reactors (PWRs) ranging from 900 MW to 1500 MW. It has also completed a significant number of export contracts that have made AREVA world leader. Nearly 600 heavy components (reactor vessels, steam generators, pressurizers and closure heads) have been manufactured or are currently being manufactured since the plant opened in 1975. The plant is at the heart of the manufacturing chain for nuclear steam supply systems (NSSS) supplied by AREVA. On the basis of engineering data, the plant manufactures reactor vessels, reactor vessel internals, steam generators, pressurizers and related components such as accumulators, auxiliary heat exchangers and supporting elements. Vessel upper internals Other similar components such as reactor vessels for boiling water reactors (BWR) or high temperature reactors (HTR) and other types of steam generators can also be manufactured in the plant (for example Once Through Steam Generators - OTSG). The basic activities performed at Chalon/St. Marcel are metalworking and heavy machining. These activities are carried out in

  3. A Dictyostelium chalone uses G proteins to regulate proliferation

    Hanson Nana E

    2009-07-01

    Full Text Available Abstract Background Several studies have shown that organ size, and the proliferation of tumor metastases, may be regulated by negative feedback loops in which autocrine secreted factors called chalones inhibit proliferation. However, very little is known about chalones, and how cells sense them. We previously identified two secreted proteins, AprA and CfaD, which act as chalones in Dictyostelium. Cells lacking AprA or CfaD proliferate faster than wild-type cells, and adding recombinant AprA or CfaD to cells slows their proliferation. Results We show here that cells lacking the G protein components Galpha8, Galpha9, and Gbeta proliferate faster than wild-type cells despite secreting normal or high levels of AprA and CfaD. Compared with wild-type cells, the proliferation of galpha8-, galpha9- and gbeta- cells are only weakly inhibited by recombinant AprA (rAprA. Like AprA and CfaD, Galpha8 and Gbeta inhibit cell proliferation but not cell growth (the rate of increase in mass and protein per nucleus, whereas Galpha9 inhibits both proliferation and growth. galpha8- cells show normal cell-surface binding of rAprA, whereas galpha9- and gbeta- cells have fewer cell-surface rAprA binding sites, suggesting that Galpha9 and Gbeta regulate the synthesis or processing of the AprA receptor. Like other ligands that activate G proteins, rAprA induces the binding of [3H]GTP to membranes, and GTPgammaS inhibits the binding of rAprA to membranes. Both AprA-induced [3H]GTP binding and the GTPgammaS inhibition of rAprA binding require Galpha8 and Gbeta but not Galpha9. Like aprA- cells, galpha8- cells have reduced spore viability. Conclusion This study shows that Galpha8 and Gbeta are part of the signal transduction pathway used by AprA to inhibit proliferation but not growth in Dictyostelium, whereas Galpha9 is part of a differealnt pathway that regulates both proliferation and growth, and that a chalone signal transduction pathway uses G proteins.

  4. A Dictyostelium chalone uses G proteins to regulate proliferation.

    Bakthavatsalam, Deenadayalan; Choe, Jonathan M; Hanson, Nana E; Gomer, Richard H

    2009-07-27

    Several studies have shown that organ size, and the proliferation of tumor metastases, may be regulated by negative feedback loops in which autocrine secreted factors called chalones inhibit proliferation. However, very little is known about chalones, and how cells sense them. We previously identified two secreted proteins, AprA and CfaD, which act as chalones in Dictyostelium. Cells lacking AprA or CfaD proliferate faster than wild-type cells, and adding recombinant AprA or CfaD to cells slows their proliferation. We show here that cells lacking the G protein components Galpha8, Galpha9, and Gbeta proliferate faster than wild-type cells despite secreting normal or high levels of AprA and CfaD. Compared with wild-type cells, the proliferation of galpha8-, galpha9- and gbeta- cells are only weakly inhibited by recombinant AprA (rAprA). Like AprA and CfaD, Galpha8 and Gbeta inhibit cell proliferation but not cell growth (the rate of increase in mass and protein per nucleus), whereas Galpha9 inhibits both proliferation and growth. galpha8- cells show normal cell-surface binding of rAprA, whereas galpha9- and gbeta- cells have fewer cell-surface rAprA binding sites, suggesting that Galpha9 and Gbeta regulate the synthesis or processing of the AprA receptor. Like other ligands that activate G proteins, rAprA induces the binding of [3H]GTP to membranes, and GTPgammaS inhibits the binding of rAprA to membranes. Both AprA-induced [3H]GTP binding and the GTPgammaS inhibition of rAprA binding require Galpha8 and Gbeta but not Galpha9. Like aprA- cells, galpha8- cells have reduced spore viability. This study shows that Galpha8 and Gbeta are part of the signal transduction pathway used by AprA to inhibit proliferation but not growth in Dictyostelium, whereas Galpha9 is part of a differealnt pathway that regulates both proliferation and growth, and that a chalone signal transduction pathway uses G proteins.

  5. eIF2α Kinases Control Chalone Production in Dictyostelium discoideum ▿

    Bowman, Robert L.; Xiong, Yanhua; Kirsten, Janet H.; Singleton, Charles K.

    2011-01-01

    Growing Dictyostelium cells secrete CfaD and AprA, two proteins that have been characterized as chalones. They exist within a high-molecular-weight complex that reversibly inhibits cell proliferation, but not growth, via cell surface receptors and a signaling pathway that includes G proteins. How the production of these two proteins is regulated is unknown. Dictyostelium cells possess three GCN2-type eukaryotic initiation factor 2 α subunit (eIF2α) kinases, proteins that phosphorylate the translational initiation factor eIF2α and possess a tRNA binding domain involved in their regulation. The Dictyostelium kinases have been shown to function during development in regulating several processes. We show here that expression of an unregulated, activated kinase domain greatly inhibits cell proliferation. The inhibitory effect on proliferation is not due to a general inhibition of translation. Instead, it is due to enhanced production of a secreted factor(s). Indeed, extracellular CfaD and AprA proteins, but not their mRNAs, are overproduced in cells expressing the activated kinase domain. The inhibition of proliferation is not seen when the activated kinase domain is expressed in cells lacking CfaD or AprA or in cells that contain a nonphosphorylatable eIF2α. We conclude that production of the chalones CfaD and AprA is translationally regulated by eIF2α phosphorylation. Both proteins are upregulated at the culmination of development, and this enhanced production is lacking in a strain that possesses a nonphosphorylatable eIF2α. PMID:21278229

  6. 30% of abatement of coldness needs + free re-heating at the skating rink of Chalons-en-Champagne; 30% de reduction des besoins de froid + rechauffage gratuit a la patinoire de Chalon-en-Champagne

    Anon.

    2005-12-01

    The skating rink of Chalons-en-Champagne (France) is equipped with a air treatment system based on the use of a sensible energy transfer battery which reduces by 30% the coldness needs for dehumidification. This process uses part of the heat released by the condensers to generate ice. The result is a 30% reduction of the installed power and a 40% reduction of energy consumptions with respect to standard air treatment solutions. (J.S.)

  7. Areva, Chalon/St-Marcel site, Environment, social and societal report for 2010-2011

    2012-08-01

    After a brief indication of Areva's activities and a map locating Areva's implantations, and the distribution of turnover and personnel in the different continents, a brief recall of safety commitments, and a presentation of the activity of the Reactors and Services Business Group (turnover, personnel, number of reactors in charge), this report briefly presents the different AREVA sites in Burgundy, and more precisely the Chalon/St-Marcel site which comprises a technical centre and a plant of production of heavy components. The activities of the technical centre concern welding technology, nuclear fusion, corrosion and chemistry, fluid and structure mechanics, and technologies related to the development of new energies and to the environment. Fields of intervention and clients are evoked. The activity of the plant of production of heavy components (vapor generators, pressurizers, vessels, etc.) and some characteristics of these components are presented. The environmental aspect is then addressed: evolution of resource consumption (gas, water, electricity, paper), and of releases (greenhouse gases, used waters, waste management). The social aspect comprises social relationships, health (involved actors, prevention, safety, evolution of labour accidents), ability and job management (training, career management, education). The societal commitments have been acknowledged by several labels related to social inclusion and local activities, support of sporting and cultural activities

  8. An attempt to distinguish a modified genetic response of the mouse testis to X-ray exposure by the action of a spermatogonial chalone

    Cattanach, B.M.; Jones, J.T.; Andrews, S.J.; Crocker, M.

    1979-01-01

    The results of an experiment designed to distinguish whether the action of a spermatogonial chalone in the mouse testis could modify the genetic response of a depleted stem spermatogonial population to X-radiation are reported. The results are consistent with the view that the stem cell population of the depleted adult testis a few days after damage closely approximates that of the early post-natal or immature animal, do not provide any indication that the testis extract in any way influence the response of the depleted testis to the 500-rad challenging dose. The yield of genetic damage was almost identical to that in the two control groups and the sterile period and testis weight data provided little reason to suspect that the amount of spermatogonial killing was altered. (Auth.)

  9. 27 CFR 9.24 - Chalone.

    2010-04-01

    ..., in their entirety, the western half of Section 5, and the eastern half of Section 18 of T.17 S., R.7 E.; and (4) Section 31, in its entirety, and the western half of Section 32 of T.16 S., R.7 E. [T.D...

  10. A retinoblastoma orthologue is required for the sensing of a chalone in Dictyostelium discoideum.

    Bakthavatsalam, Deenadayalan; White, Michael J V; Herlihy, Sarah E; Phillips, Jonathan E; Gomer, Richard H

    2014-03-01

    Retinoblastoma-like proteins regulate cell differentiation and inhibit cell proliferation. The Dictyostelium discoideum retinoblastoma orthologue RblA affects the differentiation of cells during multicellular development, but it is unclear whether RblA has a significant effect on Dictyostelium cell proliferation, which is inhibited by the secreted proteins AprA and CfaD. We found that rblA⁻ cells in shaking culture proliferate to a higher density, die faster after reaching stationary density, and, after starvation, have a lower spore viability than wild-type cells, possibly because in shaking culture, rblA⁻ cells have both increased cytokinesis and lower extracellular accumulation of CfaD. However, rblA⁻ cells have abnormally slow proliferation on bacterial lawns. Recombinant AprA inhibits the proliferation of wild-type cells but not that of rblA⁻ cells, whereas CfaD inhibits the proliferation of both wild-type cells and rblA⁻ cells. Similar to aprA⁻ cells, rblA⁻ cells have a normal mass and protein accumulation rate on a per-nucleus basis, indicating that RblA affects cell proliferation but not cell growth. AprA also functions as a chemorepellent, and RblA is required for proper AprA chemorepellent activity despite the fact that RblA does not affect cell speed. Together, our data indicate that an autocrine proliferation-inhibiting factor acts through RblA to regulate cell density in Dictyostelium, suggesting that such factors may signal through retinoblastoma-like proteins to control the sizes of structures such as developing organs or tumors.

  11. Control of cell division and radiation injury in mouse skin

    Yamaguchi, Takeo

    1974-01-01

    The method for determining the inhibitors of cell division (chalone-adrenalin system) in the irradiated epidermis and blood was developed using the epidermis of mouse ear conch during the cure of wounds (in vivo), and the epidermis cultured for a long period (in vitro). The whole body was irradiated with 200KV, 20 mA x-rays of 96 R/min filtered by 0.5 mmCu + 0.5 mmAl. Chalone, which is a physiologically intrinsic substance to control the proliferation, inhibits the DNA synthesis. From changes in cell division with time, chalone in the epidermis is considered to inhibit each process from G 2 to M, from G 2 to S, from G 1 to S. Adrenalin is indispensable when epidermal chalone acts the inhibition of cell division. Chalone activities in the epidermis irradiated with almost lethal doses were decreased. Factors to inhibit the proliferation of the epidermis by the potentiation of chalone and adrenalin are present in sera of animals irradiated to x-rays. (Serizawa, K.)

  12. The Creys Malville FBR Super Phenix steam generators

    Baque, P.; Zuber, T.; Saur, J.M.; Cambillard, E.

    1980-08-01

    After briefly recalling the French experience on sodium steam generators, the authors describe the design concepts of the Superphenix units and give their main characteristics. A short summary of the realized R and D program precedes the description of the four 750-MWt steam generators, the fabrication of which is in progress by Creusot-Loire at Chalon sur Saone (France). The studies started for the next French fast breeder reactors and their steam generators are mentioned

  13. Characterization of Airborne Particles in the Baroque Hall of the National Library in Prague

    Mašková, Ludmila; Smolík, Jiří; Džumbová, Lucie; Ondráček, Jakub; López-Aparicio, S.; Grontoft, T.; Stankiewicz, J.

    -, č. 7 (2010), s. 141-146 ISSN 1581-9280. [9th Indoor Air Quality Meeting. Chalon-sur-Saone, 21.04.2010-23.04.2010] R&D Projects: GA MŠk OC09049 Grant - others:MF NF(CZ) A/CZ0046/2/0001 Institutional research plan: CEZ:AV0Z40720504 Keywords : indoor /outdoor aerosol * mass size distribution * chemical composition Subject RIV: CF - Physical ; Theoretical Chemistry

  14. A secreted factor represses cell proliferation in Dictyostelium

    Brock, Debra A.; Gomer, Richard H.

    2005-01-01

    Many cells appear to secrete factors called chalones that limit their proliferation, but in most cases the factors have not been identified. We found that growing Dictyostelium cells secrete a 60 kDa protein called AprA for autocrine proliferation repressor. AprA has similarity to putative bacterial proteins of unknown function. Compared with wild-type cells, aprA-null cells proliferate faster, while AprA overexpressing cells proliferate slower. Growing wild-type cells secrete a factor that i...

  15. Rumors from the world

    Anon.

    2007-01-01

    23.1% electric power are from nuclear energy in the OECD countries in 2006. The first forged piece devoted to one of the four vapor generators of the first evolutionary power reactor in Usa has been delivered to the factory of Chalon (France) of Areva group. British energy wants to extend its nuclear power plants lifetime. In belgium the nuclear power plants shutdown could be called in question again. The Brazil government wants to build a third nuclear power plant whereas this project was suspended for more than twenty years. Two french groups, Bouygues and Vinci have signed the contract for the Chernobylsk sarcophagus. (N.C.)

  16. A secreted factor represses cell proliferation in Dictyostelium.

    Brock, Debra A; Gomer, Richard H

    2005-10-01

    Many cells appear to secrete factors called chalones that limit their proliferation, but in most cases the factors have not been identified. We found that growing Dictyostelium cells secrete a 60 kDa protein called AprA for autocrine proliferation repressor. AprA has similarity to putative bacterial proteins of unknown function. Compared with wild-type cells, aprA-null cells proliferate faster, while AprA overexpressing cells proliferate slower. Growing wild-type cells secrete a factor that inhibits the proliferation of wild-type and aprA- cells; this activity is not secreted by aprA- cells. AprA purified by immunoprecipitation also slows the proliferation of wild-type and aprA- cells. Compared with wild type, there is a higher percentage of multinucleate cells in the aprA- population, and when starved, aprA- cells form abnormal structures that contain fewer spores. AprA may thus decrease the number of multinucleate cells and increase spore production. Together, the data suggest that AprA functions as part of a Dictyostelium chalone.

  17. Panorama de l’écrit diplomatique en Bourgogne : autour des cartulaires (XIe-XVIIIe siècles

    Isabelle Rosé

    2007-04-01

    Full Text Available IntroductionL’écrit diplomatique en Bourgogne se caractérise d’abord par l’importance de la masse documentaire existante, ne serait-ce, pour prendre un exemple emblématique, que par l’énorme fonds de Cluny. Selon un premier bilan, encore provisoire mais qui sera sans aucun doute réévalué à la hausse, au moins 374 cartulaires ont été confectionnés dans l’espace géographique qui correspond aux diocèses d’Auxerre, Autun, Chalon, Nevers, Mâcon et Langres, Besançon et Sens . Sur cet ensemble, 316 ...

  18. Panorama de l’écrit diplomatique en Bourgogne : autour des cartulaires (XIe-XVIIIe siècles

    Isabelle Rosé

    2008-06-01

    Full Text Available IntroductionL’écrit diplomatique en Bourgogne se caractérise d’abord par l’importance de la masse documentaire existante, ne serait-ce, pour prendre un exemple emblématique, que par l’énorme fonds de Cluny. Selon un premier bilan, encore provisoire mais qui sera sans aucun doute réévalué à la hausse, au moins 374 cartulaires ont été confectionnés dans l’espace géographique qui correspond aux diocèses d’Auxerre, Autun, Chalon, Nevers, Mâcon et Langres, Besançon et Sens . Sur cet ensemble, 316 ...

  19. An experimental and mathematical analysis of lymphopoiesis dynamics under continuous irradiation

    Zukhbaya, T.M.; Smirnova, O.A.

    1991-01-01

    A mathematical model describing the dynamics of lymphopoiesis in mammals continuously exposed to ionizing radiation has been developed. It is based on the theory of chalone regulation of hematopoiesis. The model comprises a system of nine differential equations. Results from the model were compared with our experimental data for bone marrow and blood lymphocytes of rats continuously exposed to gamma radiation in a wide range of dose rates. The model reproduces the lymphopoiesis dynamics that we observed in our experiment, in particular, the radiation hormesis at low dose rates, the reduction of lymphopoiesis at intermediate dose rates, and extinction of lymphopoiesis at high dose rates of continuous radiation. The possible explanation of the hormesis is suggested by the framework of the model. The model can be used for predicting the lymphopoiesis dynamics in mammals under continuous irradiation

  20. Growth regulation in X-irradiated mouse skin

    Elgjo, K.; Devik, F.

    1978-01-01

    Extracts of hairless mouse skin were tested for their content of epidermal G 1 inhibitor and G 2 inhibitor at daily intervals after X-irradiation with 4 500 or 2 250 rad. After either dose the skin extracts lacked G 1 inhibitory activity on days 5 and 6 respectively after irradiation. This coincided with the time when the epidermal mitotic rate again became normal and started a period of over-shoot. The time interval of 5 to 6 days corresponds to the turnover time of the differentiating cells in hairless mouse back epidermis. The findings indicate that the proliferating cells in epidermis can respond to changes in local chalone concentration, even after X-irradiation at the tested doses, and that the irradiated epidermal cell population still retains some important properties inherent in a cybernetically regulated system. The local G 2 -inhibitory activity also varied after irradiation, but these variations could not be directly related to the corresponding mitotic rates. (author)

  1. Development and introduction of a modular training and qualification concept for maintenance personnel

    Kumpf, Thomas; Mueller, Nina; Hofbauer, Detlef

    2009-01-01

    The presentation entitled ''Development and Introduction of a Modular Training and Qualification Concept for Maintenance Personnel'' presents the background, concept and training modules of a reactor services training concept that was jointly elaborated by a working group consisting of the ''Maintenance manager workshop'' working panel of the VGB, the Kraftwerksschule (KWS) PowerTech Training Center, and AREVA NP GmbH in Erlangen and Offenbach. This concept is part of AREVA's comprehensive training and qualification approach which comprises inter alia extensive introduction sessions for new employees, the corporation-wide highly appreciated AREVA University as well as specialized training facilities as for example the CETIC in Chalon-sur-Saone, France and the training center in Lynchburg, USA, or the worldwide largest integration center for digital I and C equipment for safety applications in nuclear power plants in Erlangen. AREVA NP's training concept for maintenance personnel addresses not only employees from AREVA NP working in nuclear maintenance but also personnel from nuclear power plants as well as independent experts, representatives from authorities and other interest groups. (orig.)

  2. Plant-derived health: the effects of turmeric and curcuminoids.

    Bengmark, S; Mesa, M D; Gil, A

    2009-01-01

    Plants contain numerous polyphenols, which have been shown to reduce inflammation and hereby to increase resistance to disease. Examples of such polyphenols are isothiocyanates in cabbage and broccoli, epigallocatechin in green tee, capsaicin in chili peppers, chalones, rutin and naringenin in apples, resveratrol in red wine and fresh peanuts and curcumin/curcuminoids in turmeric. Most diseases are maintained by a sustained discreet but obvious increased systemic inflammation. Many studies suggest that the effect of treatment can be improved by a combination of restriction in intake of proinflammatory molecules such as advanced glycation end products (AGE), advanced lipoperoxidation end products (ALE), and rich supply of antiinflammatory molecules such as plant polyphenols. To the polyphenols with a bulk of experimental documentation belong the curcuminoid family and especially its main ingredient, curcumin. This review summarizes the present knowledge about these turmericderived ingredients, which have proven to be strong antioxidants and inhibitors of cyclooxigenase-2 (COX-2), lipoxygenase (LOX) and nuclear factor kappa B (NF-kappaB) but also AGE. A plethora of clinical effects are reported in various experimental diseases, but clinical studies in humans are few. It is suggested that supply of polyphenols and particularly curcuminoids might be value as complement to pharmaceutical treatment, but also prebiotic treatment, in conditions proven to be rather therapy-resistant such as Crohn's, long-stayed patients in intensive care units, but also in conditions such as cancer, liver cirrhosis, chronic renal disease, chronic obstructive lung disease, diabetes and Alzheimer's disease.

  3. News from the world

    Anon.

    2005-01-01

    This document gathers a series of very short articles concerning nuclear industry around the world. Areva company is investing 30 million euros in its Chalon-Saint-Marcel plant, it is the consequence of the extension of service life of nuclear power plants in the Usa. Areva holds 40% of the American market concerning the replacement of steam generators and 50% of that concerning the replacement of closure heads. The Obrigheim nuclear power plant was definitely closed down on may 2004, this decommissioning is a step forward in the German policy of progressively stepping out of nuclear energy. Chinese authorities are willing to construct 40 nuclear reactors in 15 years, despite that, the contribution of nuclear energy to the generation of electricity will reach only 4% in 2020. In 2007 Cea will begin the construction works of a new research reactor (Jules Horowitz reactor) in the Cadarache site. Prices of the yellow cake have been going up during the last years: from 9 dollar the pound in 2001 to 23 dollar today. Nuclear fuel manufacturers do not worry because far higher prices have been previously reached (70 dollars in the seventies) and uranium reserves are expected to assure 200 years of electricity generation at the same level of production as today. (A.C.)

  4. High-fat diet reduces local myostatin-1 paralog expression and alters skeletal muscle lipid content in rainbow trout, Oncorhynchus mykiss

    Galt, Nicholas J.; Froehlich, Jacob Michael; Meyer, Ben M.; Barrows, Frederic T.; Biga, Peggy R.

    2014-01-01

    Muscle growth is an energetically demanding process that is reliant on intramuscular fatty acid depots in most fishes. The complex mechanisms regulating this growth and lipid metabolism are of great interest for human health and aquaculture applications. It is well established that the skeletal muscle chalone, myostatin, plays a role in lipid metabolism and adipogenesis in mammals; however, this function has not been fully assessed in fishes. We therefore examined the interaction between dietary lipid levels and myostatin expression in rainbow trout (Oncorhynchus mykiss). Five-weeks of high-fat (HFD; 25% lipid) dietary intake increased white muscle lipid content, and decreased circulating glucose levels and hepatosomatic index when compared to low-fat diet (LFD; 10% lipid) intake. In addition HFD intake reduced myostatin-1a and -1b expression in white muscle and myostatin-1b expression in brain tissue. Characterization of the myostatin-1a, -1b, and -2a promoters revealed putative binding sites for a subset of transcription factors associated with lipid metabolism. Taken together, these data suggest that HFD may regulate myostatin expression through cis-regulatory elements sensitive to increased lipid intake. Further, these findings provide a framework for future investigations of mechanisms describing the relationships between myostatin and lipid metabolism in fish. PMID:24264425

  5. Effects of humoral factors on amplification of nonrecognizable erythrocytic and granulocytic precursors

    Cronkite, E.P.; Carsten, A.L.; Cohen, R.; Miller, M.E.; Moccia, G.

    1978-01-01

    The purpose of these studies was to evaluate the effects of humoral factors on amplification of nonrecognizable erythrocytic and granulocytic precursors using the in vivo plasma clot diffusion chamber and the in vitro plasma clot culture methods. Plasma erythropoietin levels changes in the reticulocyte concentration and hematocrits of irradiated and non-irradiated Long-Evans rats exposed to hypoxia were also determined. While erythropoietin plasma levels appeared to effect BFU-E and CFU-E growth, results suggest erythropoietin may not be the sole regulator of red cell production and that inhibitors or chalone-like mechanisms may be involved. Measurements made on granulocyte precursors treated with CSF containing L-cell conditioned medium revealed granulocytic colonies and burst-like formations, similar to those seen for erythrocytic growth. There is strong evidence suggesting that CSF is a regulator of granulopoiesis; however, it is not the sole regulator and it appears that inhibitors may play an in vivo role. Growth of colonies with cell numbers not a power of 2 implies either asymmetric nitosis due to loss of genetic information required for continuing division, or differences in concentration of, or ability to recognize inhibitory factors. These possibilities are examined on the basis of results using in vivo and in vitro culture techniques

  6. Delayed postural control during self-generated perturbations in the frail older adults

    Kubicki A

    2012-02-01

    Full Text Available Alexandre Kubicki1–3, François Bonnetblanc1,2, Geoffroy Petrement3, Yves Ballay1,2, France Mourey2,4¹UFR STAPS, Université de Bourgogne, Dijon, France; ²Motricité et Plasticité, Institut National de la Santé et de la Recherche Médicale (INSERM, Dijon, France; ³SARL Fovea Interactive, Campus Industriel – Espace Entreprises, Chalon sur Saône, France; 4UFR Médecine, Université de Bourgogne, Dijon, FrancePurpose: The aim of this study was to investigate the coordination between posture and movement in pathological aging (frailty in comparison with normal aging, with the hypothesis that in pathological aging, postural control evolves towards a more reactive mode for which the perturbation induced by the movement is not anticipated and leads to delayed and late postural adjustments.Methods: Elderly subjects performed rapid focal arm-raising movements towards a target, from an upright standing position in two stimuli conditions: simple reaction time and choice reaction time (CRT. Hand and center of pressure (CoP kinematics were compared between a control group and a frail group of the same age.Results: In frail individuals, the entire movement was impaired and slowed down. In addition, postural adjustments that classically precede and accompany the focal arm movement were delayed and reduced, especially in the CRT condition in which the motor prediction is more limited. Finally, a correlation between the time to CoP maximal velocity and the timed up-and-go score was observed.Conclusion: In these patients, it was concluded that the control of the CoP displacement evolved from a proactive mode in which the perturbation associated with the arm movement is anticipated toward a more reactive mode in which the perturbation is compensated by late and delayed adjustments.Keywords: frailty, anticipatory postural adjustments, backward disequilibrium

  7. Cyclic dermal BMP signalling regulates stem cell activation during hair regeneration.

    Plikus, Maksim V; Mayer, Julie Ann; de la Cruz, Damon; Baker, Ruth E; Maini, Philip K; Maxson, Robert; Chuong, Cheng-Ming

    2008-01-17

    In the age of stem cell engineering it is critical to understand how stem cell activity is regulated during regeneration. Hairs are mini-organs that undergo cyclic regeneration throughout adult life, and are an important model for organ regeneration. Hair stem cells located in the follicle bulge are regulated by the surrounding microenvironment, or niche. The activation of such stem cells is cyclic, involving periodic beta-catenin activity. In the adult mouse, regeneration occurs in waves in a follicle population, implying coordination among adjacent follicles and the extrafollicular environment. Here we show that unexpected periodic expression of bone morphogenetic protein 2 (Bmp2) and Bmp4 in the dermis regulates this process. This BMP cycle is out of phase with the WNT/beta-catenin cycle, thus dividing the conventional telogen into new functional phases: one refractory and the other competent for hair regeneration, characterized by high and low BMP signalling, respectively. Overexpression of noggin, a BMP antagonist, in mouse skin resulted in a markedly shortened refractory phase and faster propagation of the regenerative wave. Transplantation of skin from this mutant onto a wild-type host showed that follicles in donor and host can affect their cycling behaviours mutually, with the outcome depending on the equilibrium of BMP activity in the dermis. Administration of BMP4 protein caused the competent region to become refractory. These results show that BMPs may be the long-sought 'chalone' inhibitors of hair growth postulated by classical experiments. Taken together, results presented in this study provide an example of hierarchical regulation of local organ stem cell homeostasis by the inter-organ macroenvironment. The expression of Bmp2 in subcutaneous adipocytes indicates physiological integration between these two thermo-regulatory organs. Our findings have practical importance for studies using mouse skin as a model for carcinogenesis, intra-cutaneous drug

  8. Developmental roles of the BMP1/TLD metalloproteinases.

    Ge, Gaoxiang; Greenspan, Daniel S

    2006-03-01

    The astacin family (M12A) of the metzincin subclan MA(M) of metalloproteinases has been detected in developing and mature individuals of species that range from hydra to humans. Functions of this family of metalloproteinase vary from digestive degradation of polypeptides, to biosynthetic processing of extracellular proteins, to activation of growth factors. This review will focus on a small subgroup of the astacin family; the bone morphogenetic protein 1 (BMP1)/Tolloid (TLD)-like metalloproteinases. In vertebrates, the BMP1/TLD-like metalloproteinases play key roles in regulating formation of the extracellular matrix (ECM) via biosynthetic processing of various precursor proteins into mature functional enzymes, structural proteins, and proteins involved in initiating mineralization of the ECM of hard tissues. Roles in ECM formation include: processing of the C-propeptides of procollagens types I-III, to yield the major fibrous components of vertebrate ECM; proteolytic activation of the enzyme lysyl oxidase, necessary to formation of covalent cross-links in collagen and elastic fibers; processing of NH2-terminal globular domains and C-propeptides of types V and XI procollagen chains to yield monomers that are incorporated into and control the diameters of collagen type I and II fibrils, respectively; processing of precursors for laminin 5 and collagen type VII, both of which are involved in securing epidermis to underlying dermis; and maturation of small leucine-rich proteoglycans. The BMP1/TLD-related metalloproteinases are also capable of activating the vertebrate transforming growth factor-beta (TGF-beta)-like "chalones" growth differentiation factor 8 (GDF8, also known as myostatin), and GDF11 (also known as BMP11), involved in negative feedback inhibition of muscle and neural tissue growth, respectively; by freeing them from noncovalent latent complexes with their cleaved prodomains. BMP1/TLD-like proteinases also liberate the vertebrate TGF

  9. Development of the intermediate heat exchanger (IHX) for Antares

    Breuil, Eric; Francois, Gilles; Tochon, Patrice; Walle, Eric

    2006-01-01

    The plate type IHX is the most challenging component for ANTARES due to the high temperature of helium at the core outlet (from 850 deg. C to 1000 deg. C). It is indeed a first of the kind as there is no past experience in nuclear or outside nuclear industries of such a component. It requires high performance materials and high technology manufacturing processes. It is also a key component regarding the efficiency and competitiveness of the plant. Then, an intensive work program was launched in AREVA NP in order to develop this component. Different units of AREVA NP and external organisations (R and D and industrials) are involved in this development. Collaboration agreements defining the framework and the rights of each partner were elaborated when necessary. Several potential technologies are investigated in parallel with the objective to select the best one before the end of 2008. This selection will be based on design studies, cost assessment, risk assessment and technological assessment including the realisation and tests of representative mock ups. These technologies are: - Plate Machined Heat Exchanger (PMHE) with CEA and EDF; - Plate Fin Heat Exchanger (PFHE) with industrials like Nordon, Brayton Energy; - Tubular concept. Plate type IHX, which is more compact but more challenging, is the reference concept for the conceptual design phase of ANTARES but tubular concept is also investigated as a fall back solution. It must be also noted that generic R and D on high temperature materials is in progress. It includes in particular creep, corrosion and nitriding tests on samples. Two alloys were selected (230 and 617) and the final selection of one alloy is planned for the end of 2006. PMHE is one of the most promising technologies. The plates are machined by high speed machining or electro chemical etching. First machining tests performed by the AREVA NP technical centre in Chalon are encouraging but the geometry of the drills still have to be optimised in order

  10. The prevention of benign prostatic hyperplasia (bph).

    Roper, W G

    2017-03-01

    . Cadherins involved in cell adhesion and possibly chalone which exerts negative effects, must also be considered. Overall, the degree of BPH that any older male develops is governed largely by: Apart from providing the existing huge evidence for infection in BPH, this manuscript explains the production, release and absorption of PLD into the prostate gland where it is converted to LPA. This is a growth factor and acts within the prostate both on smooth muscle (research proof given) and on prostatic connective tissue, resulting in BPH. This manuscript indicates the vital research, including the requisite materials, which would readily provide irrefutable and final etiological proof of all of the hormones involved. It explains how, to a large extent, the use of a vaccine which is now being developed, would likely allow the ultimate prevention of this ubiquitous disease. Copyright © 2016 Elsevier Ltd. All rights reserved.