WorldWideScience

Sample records for cgh array designed

  1. Application of custom-designed oligonucleotide array CGH in 145 patients with autistic spectrum disorders

    OpenAIRE

    Wiśniowiecka-Kowalnik, Barbara; Kastory-Bronowska, Monika; Bartnik, Magdalena; Derwińska, Katarzyna; Dymczak-Domini, Wanda; Szumbarska, Dorota; Ziemka, Ewa; Szczałuba, Krzysztof; Sykulski, Maciej; Gambin, Tomasz; Gambin, Anna; Shaw, Chad A.; Mazurczak, Tadeusz; Obersztyn, Ewa; Bocian, Ewa

    2012-01-01

    Autism spectrum disorders (ASDs) are a heterogeneous group of neurodevelopmental disorders, including childhood autism, atypical autism, and Asperger syndrome, with an estimated prevalence of 1.0–2.5% in the general population. ASDs have a complex multifactorial etiology, with genetic causes being recognized in only 10–20% of cases. Recently, copy-number variants (CNVs) have been shown to contribute to over 10% of ASD cases. We have applied a custom-designed oligonucleotide array comparative ...

  2. Molecular karyotyping: array CGH quality criteria for constitutional genetic diagnosis.

    Science.gov (United States)

    Vermeesch, Joris R; Melotte, Cindy; Froyen, Guy; Van Vooren, Steven; Dutta, Binita; Maas, Nicole; Vermeulen, Stefan; Menten, Björn; Speleman, Frank; De Moor, Bart; Van Hummelen, Paul; Marynen, Peter; Fryns, Jean-Pierre; Devriendt, Koen

    2005-03-01

    Array CGH (comparative genomic hybridization) enables the identification of chromosomal copy number changes. The availability of clone sets covering the human genome opens the possibility for the widespread use of array CGH for both research and diagnostic purposes. In this manuscript we report on the parameters that were critical for successful implementation of the technology, assess quality criteria, and discuss the potential benefits and pitfalls of the technology for improved pre- and postnatal constitutional genetic diagnosis. We propose to name the genome-wide array CGH "molecular karyotyping," in analogy with conventional karyotyping that uses staining methods to visualize chromosomes.

  3. Whole genome amplification and its impact on CGH array profiles

    Directory of Open Access Journals (Sweden)

    Meldrum Cliff

    2008-07-01

    Full Text Available Abstract Background Some array comparative genomic hybridisation (array CGH platforms require a minimum of micrograms of DNA for the generation of reliable and reproducible data. For studies where there are limited amounts of genetic material, whole genome amplification (WGA is an attractive method for generating sufficient quantities of genomic material from miniscule amounts of starting material. A range of WGA methods are available and the multiple displacement amplification (MDA approach has been shown to be highly accurate, although amplification bias has been reported. In the current study, WGA was used to amplify DNA extracted from whole blood. In total, six array CGH experiments were performed to investigate whether the use of whole genome amplified DNA (wgaDNA produces reliable and reproducible results. Four experiments were conducted on amplified DNA compared to unamplified DNA and two experiments on unamplified DNA compared to unamplified DNA. Findings All the experiments involving wgaDNA resulted in a high proportion of losses and gains of genomic material. Previously, amplification bias has been overcome by using amplified DNA in both the test and reference DNA. Our data suggests that this approach may not be effective, as the gains and losses introduced by WGA appears to be random and are not reproducible between different experiments using the same DNA. Conclusion In light of these findings, the use of both amplified test and reference DNA on CGH arrays may not provide an accurate representation of copy number variation in the DNA.

  4. Model-based clustering of array CGH data

    OpenAIRE

    Shah, Sohrab P.; Cheung, K-John; Johnson, Nathalie A.; Alain, Guillaume; Gascoyne, Randy D.; Horsman, Douglas E.; Ng, Raymond T.; Murphy, Kevin P.

    2009-01-01

    Motivation: Analysis of array comparative genomic hybridization (aCGH) data for recurrent DNA copy number alterations from a cohort of patients can yield distinct sets of molecular signatures or profiles. This can be due to the presence of heterogeneous cancer subtypes within a supposedly homogeneous population. Results: We propose a novel statistical method for automatically detecting such subtypes or clusters. Our approach is model based: each cluster is defined in terms of a sparse profile...

  5. CGHPRO – A comprehensive data analysis tool for array CGH

    Directory of Open Access Journals (Sweden)

    Lenzner Steffen

    2005-04-01

    Full Text Available Abstract Background Array CGH (Comparative Genomic Hybridisation is a molecular cytogenetic technique for the genome wide detection of chromosomal imbalances. It is based on the co-hybridisation of differentially labelled test and reference DNA onto arrays of genomic BAC clones, cDNAs or oligonucleotides, and after correction for various intervening variables, loss or gain in the test DNA can be indicated from spots showing aberrant signal intensity ratios. Now that this technique is no longer confined to highly specialized laboratories and is entering the realm of clinical application, there is a need for a user-friendly software package that facilitates estimates of DNA dosage from raw signal intensities obtained by array CGH experiments, and which does not depend on a sophisticated computational environment. Results We have developed a user-friendly and versatile tool for the normalization, visualization, breakpoint detection and comparative analysis of array-CGH data. CGHPRO is a stand-alone JAVA application that guides the user through the whole process of data analysis. The import option for image analysis data covers several data formats, but users can also customize their own data formats. Several graphical representation tools assist in the selection of the appropriate normalization method. Intensity ratios of each clone can be plotted in a size-dependent manner along the chromosome ideograms. The interactive graphical interface offers the chance to explore the characteristics of each clone, such as the involvement of the clones sequence in segmental duplications. Circular Binary Segmentation and unsupervised Hidden Markov Model algorithms facilitate objective detection of chromosomal breakpoints. The storage of all essential data in a back-end database allows the simultaneously comparative analysis of different cases. The various display options facilitate also the definition of shortest regions of overlap and simplify the

  6. Estimation of tumor heterogeneity using CGH array data

    Directory of Open Access Journals (Sweden)

    Li Shengting

    2009-01-01

    Full Text Available Abstract Background Array-based comparative genomic hybridization (CGH is a commonly-used approach to detect DNA copy number variation in whole genome-wide screens. Several statistical methods have been proposed to define genomic segments with different copy numbers in cancer tumors. However, most tumors are heterogeneous and show variation in DNA copy numbers across tumor cells. The challenge is to reveal the copy number profiles of the subpopulations in a tumor and to estimate the percentage of each subpopulation. Results We describe a relation between experimental data and exact DNA copy number and develop a statistical method to reveal the heterogeneity of tumors containing a mixture of different-stage cells. Furthermore, we validate the method on simulated data and apply the method to 29 pairs of breast primary tumors and their matched lymph node metastases. Conclusion We demonstrate a new method for CGH array analysis that allows a tumor sample to be classified according to its heterogeneity. The method gives an interpretable series of copy number profiles, one for each major subpopulation in a tumor. The profiles facilitate identification of copy number alterations in cancer development.

  7. Combined array CGH plus SNP genome analyses in a single assay for optimized clinical testing.

    Science.gov (United States)

    Wiszniewska, Joanna; Bi, Weimin; Shaw, Chad; Stankiewicz, Pawel; Kang, Sung-Hae L; Pursley, Amber N; Lalani, Seema; Hixson, Patricia; Gambin, Tomasz; Tsai, Chun-hui; Bock, Hans-Georg; Descartes, Maria; Probst, Frank J; Scaglia, Fernando; Beaudet, Arthur L; Lupski, James R; Eng, Christine; Cheung, Sau Wai; Bacino, Carlos; Patel, Ankita

    2014-01-01

    In clinical diagnostics, both array comparative genomic hybridization (array CGH) and single nucleotide polymorphism (SNP) genotyping have proven to be powerful genomic technologies utilized for the evaluation of developmental delay, multiple congenital anomalies, and neuropsychiatric disorders. Differences in the ability to resolve genomic changes between these arrays may constitute an implementation challenge for clinicians: which platform (SNP vs array CGH) might best detect the underlying genetic cause for the disease in the patient? While only SNP arrays enable the detection of copy number neutral regions of absence of heterozygosity (AOH), they have limited ability to detect single-exon copy number variants (CNVs) due to the distribution of SNPs across the genome. To provide comprehensive clinical testing for both CNVs and copy-neutral AOH, we enhanced our custom-designed high-resolution oligonucleotide array that has exon-targeted coverage of 1860 genes with 60,000 SNP probes, referred to as Chromosomal Microarray Analysis - Comprehensive (CMA-COMP). Of the 3240 cases evaluated by this array, clinically significant CNVs were detected in 445 cases including 21 cases with exonic events. In addition, 162 cases (5.0%) showed at least one AOH region >10 Mb. We demonstrate that even though this array has a lower density of SNP probes than other commercially available SNP arrays, it reliably detected AOH events >10 Mb as well as exonic CNVs beyond the detection limitations of SNP genotyping. Thus, combining SNP probes and exon-targeted array CGH into one platform provides clinically useful genetic screening in an efficient manner.

  8. Detection of clinically relevant exonic copy-number changes by array CGH.

    Science.gov (United States)

    Boone, Philip M; Bacino, Carlos A; Shaw, Chad A; Eng, Patricia A; Hixson, Patricia M; Pursley, Amber N; Kang, Sung-Hae L; Yang, Yaping; Wiszniewska, Joanna; Nowakowska, Beata A; del Gaudio, Daniela; Xia, Zhilian; Simpson-Patel, Gayle; Immken, LaDonna L; Gibson, James B; Tsai, Anne C-H; Bowers, Jennifer A; Reimschisel, Tyler E; Schaaf, Christian P; Potocki, Lorraine; Scaglia, Fernando; Gambin, Tomasz; Sykulski, Maciej; Bartnik, Magdalena; Derwinska, Katarzyna; Wisniowiecka-Kowalnik, Barbara; Lalani, Seema R; Probst, Frank J; Bi, Weimin; Beaudet, Arthur L; Patel, Ankita; Lupski, James R; Cheung, Sau Wai; Stankiewicz, Pawel

    2010-12-01

    Array comparative genomic hybridization (aCGH) is a powerful tool for the molecular elucidation and diagnosis of disorders resulting from genomic copy-number variation (CNV). However, intragenic deletions or duplications--those including genomic intervals of a size smaller than a gene--have remained beyond the detection limit of most clinical aCGH analyses. Increasing array probe number improves genomic resolution, although higher cost may limit implementation, and enhanced detection of benign CNV can confound clinical interpretation. We designed an array with exonic coverage of selected disease and candidate genes and used it clinically to identify losses or gains throughout the genome involving at least one exon and as small as several hundred base pairs in size. In some patients, the detected copy-number change occurs within a gene known to be causative of the observed clinical phenotype, demonstrating the ability of this array to detect clinically relevant CNVs with subkilobase resolution. In summary, we demonstrate the utility of a custom-designed, exon-targeted oligonucleotide array to detect intragenic copy-number changes in patients with various clinical phenotypes.

  9. Automated array-CGH optimized for archival formalin-fixed, paraffin-embedded tumor material

    OpenAIRE

    Nederlof Petra M; van Beers Erik H; Joosse Simon A

    2007-01-01

    Abstract Background Array Comparative Genomic Hybridization (aCGH) is a rapidly evolving technology that still lacks complete standardization. Yet, it is of great importance to obtain robust and reproducible data to enable meaningful multiple hybridization comparisons. Special difficulties arise when aCGH is performed on archival formalin-fixed, paraffin-embedded (FFPE) tissue due to its variable DNA quality. Recently, we have developed an effective DNA quality test that predicts suitability ...

  10. Improved structural characterization of chromosomal breakpoints using high resolution custom array-CGH

    NARCIS (Netherlands)

    Lindstrand, A.; Schoumans, J.; Gustavsson, P.; Hanemaaijer, N.; Malmgren, H.; Blennow, E.

    2010-01-01

    Array-CGH is a powerful tool for the rapid detection of genomic imbalances. By customizing the array it is possible to increase the resolution in a targeted genomic region of interest and determine the structure of the breakpoints with high accuracy, as well as to detect very small imbalances. We ha

  11. High-resolution SNP array analysis of patients with developmental disorder and normal array CGH results

    Directory of Open Access Journals (Sweden)

    Siggberg Linda

    2012-09-01

    Full Text Available Abstract Background Diagnostic analysis of patients with developmental disorders has improved over recent years largely due to the use of microarray technology. Array methods that facilitate copy number analysis have enabled the diagnosis of up to 20% more patients with previously normal karyotyping results. A substantial number of patients remain undiagnosed, however. Methods and Results Using the Genome-Wide Human SNP array 6.0, we analyzed 35 patients with a developmental disorder of unknown cause and normal array comparative genomic hybridization (array CGH results, in order to characterize previously undefined genomic aberrations. We detected no seemingly pathogenic copy number aberrations. Most of the vast amount of data produced by the array was polymorphic and non-informative. Filtering of this data, based on copy number variant (CNV population frequencies as well as phenotypically relevant genes, enabled pinpointing regions of allelic homozygosity that included candidate genes correlating to the phenotypic features in four patients, but results could not be confirmed. Conclusions In this study, the use of an ultra high-resolution SNP array did not contribute to further diagnose patients with developmental disorders of unknown cause. The statistical power of these results is limited by the small size of the patient cohort, and interpretation of these negative results can only be applied to the patients studied here. We present the results of our study and the recurrence of clustered allelic homozygosity present in this material, as detected by the SNP 6.0 array.

  12. CGH arrays compared for DNA isolated from formalin-fixed, paraffin-embedded material

    NARCIS (Netherlands)

    Krijgsman, Oscar; Israeli, Danielle; Haan, Josien C.; van Essen, Hendrik F.; Smeets, Serge J.; Eijk, Paul P.; Steenbergen, Renske D. M.; Kok, Klaas; Tejpar, Sabine; Meijer, Gerrit A.; Ylstra, Bauke

    2012-01-01

    Formalin-fixed, paraffin-embedded (FFPE) archival tissue is an important source of DNA material. The most commonly used technique to identify copy number aberrations from chromosomal DNA in tumorigenesis is array comparative genomic hybridization (aCGH). Although copy number analysis using DNA from

  13. Analysis of Array-CGH Data Using the R and Bioconductor Software Suite

    Directory of Open Access Journals (Sweden)

    Winfried A. Hofmann

    2009-01-01

    Full Text Available Background. Array-based comparative genomic hybridization (array-CGH is an emerging high-resolution and high-throughput molecular genetic technique that allows genome-wide screening for chromosome alterations. DNA copy number alterations (CNAs are a hallmark of somatic mutations in tumor genomes and congenital abnormalities that lead to diseases such as mental retardation. However, accurate identification of amplified or deleted regions requires a sequence of different computational analysis steps of the microarray data. Results. We have developed a user-friendly and versatile tool for the normalization, visualization, breakpoint detection, and comparative analysis of array-CGH data which allows the accurate and sensitive detection of CNAs. Conclusion. The implemented option for the determination of minimal altered regions (MARs from a series of tumor samples is a step forward in the identification of new tumor suppressor genes or oncogenes.

  14. X chromosome array-CGH for the identification of novel X-linked mental retardation genes.

    Science.gov (United States)

    Bauters, Marijke; Van Esch, Hilde; Marynen, Peter; Froyen, Guy

    2005-01-01

    Array-CGH technology for the detection of submicroscopic copy number changes in the genome has recently been developed for the identification of novel disease-associated genes. It has been estimated that submicroscopic genomic deletions or duplications will be present in 5-7% of patients with idiopathic mental retardation (MR). Since 30% more males than females are diagnosed with MR, we have developed a full coverage X chromosome array-CGH with a theoretical resolution of 82 kb, for the detection of copy number alterations in patients with suspected X-linked mental retardation (XLMR). First, we have validated the genomic location of X-derived clones through male versus female hybridisations. Next, we validated our array for efficient and reproducible detection of known alterations in XLMR patients. In all cases, we were able to detect the deletions and duplications in males as well as females. Due to the high resolution of our X-array, the boundaries of the genomic aberrations could clearly be identified making genotype-phenotype studies more reliable. Here, we describe the production and validation of a full coverage X-array-CGH, which will allow for fast and easy screening of submicroscopic copy number alterations in XLMR patients with the aim to identify novel MR genes or mechanisms involved in a deranged cognitive development.

  15. Reliable single cell array CGH for clinical samples.

    Directory of Open Access Journals (Sweden)

    Zbigniew T Czyż

    Full Text Available BACKGROUND: Disseminated cancer cells (DCCs and circulating tumor cells (CTCs are extremely rare, but comprise the precursors cells of distant metastases or therapy resistant cells. The detailed molecular analysis of these cells may help to identify key events of cancer cell dissemination, metastatic colony formation and systemic therapy escape. METHODOLOGY/PRINCIPAL FINDINGS: Using the Ampli1™ whole genome amplification (WGA technology and high-resolution oligonucleotide aCGH microarrays we optimized conditions for the analysis of structural copy number changes. The protocol presented here enables reliable detection of numerical genomic alterations as small as 0.1 Mb in a single cell. Analysis of single cells from well-characterized cell lines and single normal cells confirmed the stringent quantitative nature of the amplification and hybridization protocol. Importantly, fixation and staining procedures used to detect DCCs showed no significant impact on the outcome of the analysis, proving the clinical usability of our method. In a proof-of-principle study we tracked the chromosomal changes of single DCCs over a full course of high-dose chemotherapy treatment by isolating and analyzing DCCs of an individual breast cancer patient at four different time points. CONCLUSIONS/SIGNIFICANCE: The protocol enables detailed genome analysis of DCCs and thereby assessment of the clonal evolution during the natural course of the disease and under selection pressures. The results from an exemplary patient provide evidence that DCCs surviving selective therapeutic conditions may be recruited from a pool of genomically less advanced cells, which display a stable subset of specific genomic alterations.

  16. Insertional translocation detected using FISH confirmation of array-comparative genomic hybridization (aCGH) results.

    Science.gov (United States)

    Kang, Sung-Hae L; Shaw, Chad; Ou, Zhishuo; Eng, Patricia A; Cooper, M Lance; Pursley, Amber N; Sahoo, Trilochan; Bacino, Carlos A; Chinault, A Craig; Stankiewicz, Pawel; Patel, Ankita; Lupski, James R; Cheung, Sau Wai

    2010-05-01

    Insertional translocations (ITs) are rare events that require at least three breaks in the chromosomes involved and thus qualify as complex chromosomal rearrangements (CCR). In the current study, we identified 40 ITs from approximately 18,000 clinical cases (1:500) using array-comparative genomic hybridization (aCGH) in conjunction with fluorescence in situ hybridization (FISH) confirmation of the aCGH findings, and parental follow-up studies. Both submicroscopic and microscopically visible IT events were detected. They were divided into three major categories: (1) simple intrachromosomal and interchromosomal IT resulting in pure segmental trisomy, (2) complex IT involving more than one abnormality, (3) deletion inherited from a parent with a balanced IT resulting in pure segmental monosomy. Of the cases in which follow-up parental studies were available, over half showed inheritance from an apparently unaffected parent carrying the same unbalanced rearrangement detected in the propositi, thus decreasing the likelihood that these IT events are clinically relevant. Nevertheless, we identified six cases in which small submicroscopic events were detected involving known disease-associated genes/genomic segments and are likely to be pathogenic. We recommend that copy number gains detected by clinical aCGH analysis should be confirmed using FISH analysis whenever possible in order to determine the physical location of the duplicated segment. We hypothesize that the increased use of aCGH in the clinic will demonstrate that IT occurs more frequently than previously considered but can identify genomic rearrangements with unclear clinical significance.

  17. Mulibrey nanism: Two novel mutations in a child identified by Array CGH and DNA sequencing.

    Science.gov (United States)

    Mozzillo, Enza; Cozzolino, Carla; Genesio, Rita; Melis, Daniela; Frisso, Giulia; Orrico, Ada; Lombardo, Barbara; Fattorusso, Valentina; Discepolo, Valentina; Della Casa, Roberto; Simonelli, Francesca; Nitsch, Lucio; Salvatore, Francesco; Franzese, Adriana

    2016-08-01

    In childhood, several rare genetic diseases have overlapping symptoms and signs, including those regarding growth alterations, thus the differential diagnosis is sometimes difficult. The proband, aged 3 years, was suspected to have Silver-Russel syndrome because of intrauterine growth retardation, postnatal growth retardation, typical facial dysmorphic features, macrocephaly, body asymmetry, and bilateral fifth finger clinodactyly. Other features were left atrial and ventricular enlargement and patent foramen ovale. Total X-ray skeleton showed hypoplasia of the twelfth rib bilaterally and of the coccyx, slender long bones with thick cortex, and narrow medullary channels. The genetic investigation did not confirm Silver-Russel syndrome. At the age of 5 the patient developed an additional sign: hepatomegaly. Array CGH revealed a 147 kb deletion (involving TRIM 37 and SKA2 genes) on one allele of chromosome 17, inherited from his mother. These results suggested Mulibrey nanism. The clinical features were found to fit this hypothesis. Sequencing of the TRIM 37 gene showed a single base change at a splicing locus, inherited from his father that provoked a truncated protein. The combined use of Array CGH and DNA sequencing confirmed diagnosis of Mulibrey nanism. The large deletion involving the SKA2 gene, along with the increased frequency of malignant tumours in mulibrey patients, suggests closed monitoring for cancer of our patient and his mother. Array CGH should be performed as first tier test in all infants with multiple anomalies. The clinician should reconsider the clinical features when the genetics suggests this. © 2016 Wiley Periodicals, Inc.

  18. A New Normalizing Algorithm for BAC CGH Arrays with Quality Control Metrics

    Directory of Open Access Journals (Sweden)

    Jeffrey C. Miecznikowski

    2011-01-01

    Full Text Available The main focus in pin-tip (or print-tip microarray analysis is determining which probes, genes, or oligonucleotides are differentially expressed. Specifically in array comparative genomic hybridization (aCGH experiments, researchers search for chromosomal imbalances in the genome. To model this data, scientists apply statistical methods to the structure of the experiment and assume that the data consist of the signal plus random noise. In this paper we propose “SmoothArray”, a new method to preprocess comparative genomic hybridization (CGH bacterial artificial chromosome (BAC arrays and we show the effects on a cancer dataset. As part of our R software package “aCGHplus,” this freely available algorithm removes the variation due to the intensity effects, pin/print-tip, the spatial location on the microarray chip, and the relative location from the well plate. removal of this variation improves the downstream analysis and subsequent inferences made on the data. Further, we present measures to evaluate the quality of the dataset according to the arrayer pins, 384-well plates, plate rows, and plate columns. We compare our method against competing methods using several metrics to measure the biological signal. With this novel normalization algorithm and quality control measures, the user can improve their inferences on datasets and pinpoint problems that may arise in their BAC aCGH technology.

  19. Identification of genome-wide copy number variations among diverse pig breeds by array CGH

    Directory of Open Access Journals (Sweden)

    Li Yan

    2012-12-01

    Full Text Available Abstract Background Recent studies have shown that copy number variation (CNV in mammalian genomes contributes to phenotypic diversity, including health and disease status. In domestic pigs, CNV has been catalogued by several reports, but the extent of CNV and the phenotypic effects are far from clear. The goal of this study was to identify CNV regions (CNVRs in pigs based on array comparative genome hybridization (aCGH. Results Here a custom-made tiling oligo-nucleotide array was used with a median probe spacing of 2506 bp for screening 12 pigs including 3 Chinese native pigs (one Chinese Erhualian, one Tongcheng and one Yangxin pig, 5 European pigs (one Large White, one Pietrain, one White Duroc and two Landrace pigs, 2 synthetic pigs (Chinese new line DIV pigs and 2 crossbred pigs (Landrace × DIV pigs with a Duroc pig as the reference. Two hundred and fifty-nine CNVRs across chromosomes 1–18 and X were identified, with an average size of 65.07 kb and a median size of 98.74 kb, covering 16.85 Mb or 0.74% of the whole genome. Concerning copy number status, 93 (35.91% CNVRs were called as gains, 140 (54.05% were called as losses and the remaining 26 (10.04% were called as both gains and losses. Of all detected CNVRs, 171 (66.02% and 34 (13.13% CNVRs directly overlapped with Sus scrofa duplicated sequences and pig QTLs, respectively. The CNVRs encompassed 372 full length Ensembl transcripts. Two CNVRs identified by aCGH were validated using real-time quantitative PCR (qPCR. Conclusions Using 720 K array CGH (aCGH we described a map of porcine CNVs which facilitated the identification of structural variations for important phenotypes and the assessment of the genetic diversity of pigs.

  20. Mulibrey nanism: Two novel mutations in a child identified by Array CGH and DNA sequencing.

    Science.gov (United States)

    Mozzillo, Enza; Cozzolino, Carla; Genesio, Rita; Melis, Daniela; Frisso, Giulia; Orrico, Ada; Lombardo, Barbara; Fattorusso, Valentina; Discepolo, Valentina; Della Casa, Roberto; Simonelli, Francesca; Nitsch, Lucio; Salvatore, Francesco; Franzese, Adriana

    2016-08-01

    In childhood, several rare genetic diseases have overlapping symptoms and signs, including those regarding growth alterations, thus the differential diagnosis is sometimes difficult. The proband, aged 3 years, was suspected to have Silver-Russel syndrome because of intrauterine growth retardation, postnatal growth retardation, typical facial dysmorphic features, macrocephaly, body asymmetry, and bilateral fifth finger clinodactyly. Other features were left atrial and ventricular enlargement and patent foramen ovale. Total X-ray skeleton showed hypoplasia of the twelfth rib bilaterally and of the coccyx, slender long bones with thick cortex, and narrow medullary channels. The genetic investigation did not confirm Silver-Russel syndrome. At the age of 5 the patient developed an additional sign: hepatomegaly. Array CGH revealed a 147 kb deletion (involving TRIM 37 and SKA2 genes) on one allele of chromosome 17, inherited from his mother. These results suggested Mulibrey nanism. The clinical features were found to fit this hypothesis. Sequencing of the TRIM 37 gene showed a single base change at a splicing locus, inherited from his father that provoked a truncated protein. The combined use of Array CGH and DNA sequencing confirmed diagnosis of Mulibrey nanism. The large deletion involving the SKA2 gene, along with the increased frequency of malignant tumours in mulibrey patients, suggests closed monitoring for cancer of our patient and his mother. Array CGH should be performed as first tier test in all infants with multiple anomalies. The clinician should reconsider the clinical features when the genetics suggests this. © 2016 Wiley Periodicals, Inc. PMID:27256967

  1. False positive rate of an arrayCGH platform for single-cell preimplantation genetic screening and subsequent clinical application on day-3

    OpenAIRE

    Mir, Pere; Rodrigo, Lorena; Mercader, Amparo; Buendía Segura, Maria del Pilar; Mateu, Emilia; Milán-Sánchez, Miguel; Peinado, Vanessa; Pellicer Martínez, Antonio; Remohí Giménez, José; Simón, Carlos; Rubio Lluesa, Carmen

    2012-01-01

    In this work, false positive rate of an arrayCGH platform for its use in day-3 single-blastomere analysis was calculated. For this purpose, 38 embryos diagnosed as abnormal on day-3 by FISH were re-biopsied on day-4. Single-cell day-4 arrayCGH diagnosis was then performed. A successful amplification was obtained in 97.4 % (37/38) of the day-4 cells analysed by arrayCGH. Day-3 FISH and day-4 arrayCGH diagnosis were concordant in 35/37 cases. The two discordant embryos were spread and all the c...

  2. Use of Virtual Medium in Designing of the CGH Wave Front Generator for Aspheric Testing

    Institute of Scientific and Technical Information of China (English)

    KANG Guo-guo; XIE Jing-hui; LIU Yi

    2007-01-01

    Design method and procedures of computer-generated hologram (CGH) used for aspheric test are introduced in detail.For CGH phase calculation,virtual medium which has zero refractive index at given wavelength is used to model ideal aspheric wavefront.Reflective Fresnel zones located in a ring area concentric to the CGH structure is designed to reduce or eliminate alignment errors.Substrate figure error,pattern distortion,etching and duty cycle variations that influence the reconstructed wavefront are quantitatively analyzed in theory and corresponding error equations are obtained to guide the tolerance distribution during CGH fabricating.A design example is given and the uncertainty of measurement achieves λ/20.

  3. De novo complex intra chromosomal rearrangement after ICSI: characterisation by BACs micro array-CGH

    Directory of Open Access Journals (Sweden)

    Quimsiyeh Mazin

    2008-12-01

    Full Text Available Abstract Background In routine Assisted Reproductive Technology (ART men with severe oligozoospermia or azoospermia should be informed about the risk of de novo congenital or chromosomal abnormalities in ICSI program. Also the benefits of preimplantation or prenatal genetic diagnosis practice need to be explained to the couple. Methods From a routine ICSI attempt, using ejaculated sperm from male with severe oligozoospermia and having normal karyotype, a 30 years old pregnant woman was referred to prenatal diagnosis in the 17th week for bichorionic biamniotic twin gestation. Amniocentesis was performed because of the detection of an increased foetal nuchal translucency for one of the fetus by the sonographic examination during the 12th week of gestation (WG. Chromosome and DNA studies of the fetus were realized on cultured amniocytes Results Conventional, molecular cytogenetic and microarray CGH experiments allowed us to conclude that the fetus had a de novo pericentromeric inversion associated with a duplication of the 9p22.1-p24 chromosomal region, 46,XY,invdup(9(p22.1p24 [arrCGH 9p22.1p24 (RP11-130C19 → RP11-87O1x3]. As containing the critical 9p22 region, our case is in coincidence with the general phenotype features of the partial trisomy 9p syndrome with major growth retardation, microcephaly and microretrognathia. Conclusion This de novo complex chromosome rearrangement illustrates the possible risk of chromosome or gene defects in ICSI program and the contribution of array-CGH for mapping rapidly de novo chromosomal imbalance.

  4. De novo complex intra chromosomal rearrangement after ICSI: characterisation by BACs micro array-CGH

    Science.gov (United States)

    Kasakyan, Serdar; Lohmann, Laurence; Aboura, Azeddine; Quimsiyeh, Mazin; Menezo, Yves; Tachdjian, Gerard; Benkhalifa, Moncef

    2008-01-01

    Background In routine Assisted Reproductive Technology (ART) men with severe oligozoospermia or azoospermia should be informed about the risk of de novo congenital or chromosomal abnormalities in ICSI program. Also the benefits of preimplantation or prenatal genetic diagnosis practice need to be explained to the couple. Methods From a routine ICSI attempt, using ejaculated sperm from male with severe oligozoospermia and having normal karyotype, a 30 years old pregnant woman was referred to prenatal diagnosis in the 17th week for bichorionic biamniotic twin gestation. Amniocentesis was performed because of the detection of an increased foetal nuchal translucency for one of the fetus by the sonographic examination during the 12th week of gestation (WG). Chromosome and DNA studies of the fetus were realized on cultured amniocytes Results Conventional, molecular cytogenetic and microarray CGH experiments allowed us to conclude that the fetus had a de novo pericentromeric inversion associated with a duplication of the 9p22.1-p24 chromosomal region, 46,XY,invdup(9)(p22.1p24) [arrCGH 9p22.1p24 (RP11-130C19 → RP11-87O1)x3]. As containing the critical 9p22 region, our case is in coincidence with the general phenotype features of the partial trisomy 9p syndrome with major growth retardation, microcephaly and microretrognathia. Conclusion This de novo complex chromosome rearrangement illustrates the possible risk of chromosome or gene defects in ICSI program and the contribution of array-CGH for mapping rapidly de novo chromosomal imbalance. PMID:19105807

  5. A robust method to analyze copy number alterations of less than 100 kb in single cells using oligonucleotide array CGH.

    Directory of Open Access Journals (Sweden)

    Birte Möhlendick

    Full Text Available Comprehensive genome wide analyses of single cells became increasingly important in cancer research, but remain to be a technically challenging task. Here, we provide a protocol for array comparative genomic hybridization (aCGH of single cells. The protocol is based on an established adapter-linker PCR (WGAM and allowed us to detect copy number alterations as small as 56 kb in single cells. In addition we report on factors influencing the success of single cell aCGH downstream of the amplification method, including the characteristics of the reference DNA, the labeling technique, the amount of input DNA, reamplification, the aCGH resolution, and data analysis. In comparison with two other commercially available non-linear single cell amplification methods, WGAM showed a very good performance in aCGH experiments. Finally, we demonstrate that cancer cells that were processed and identified by the CellSearch® System and that were subsequently isolated from the CellSearch® cartridge as single cells by fluorescence activated cell sorting (FACS could be successfully analyzed using our WGAM-aCGH protocol. We believe that even in the era of next-generation sequencing, our single cell aCGH protocol will be a useful and (cost- effective approach to study copy number alterations in single cells at resolution comparable to those reported currently for single cell digital karyotyping based on next generation sequencing data.

  6. The development of a mini-array for estimating the disease state of gastric adenocarcinoma by array CGH

    Directory of Open Access Journals (Sweden)

    Oga Atsunori

    2008-12-01

    Full Text Available Abstract Background The treatment strategy usually depends on the disease state in the individual patient. However, it is difficult to estimate the disease state before treatment in many patients. The purpose of this study was to develop a BAC (bacterial artificial chromosome mini-array allowing for the estimation of node metastasis, liver metastasis, peritoneal dissemination and the depth of tumor invasion in gastric cancers. Methods Initially, the DNA copy number aberrations (DCNAs were analyzed by array-based comparative genomic hybridization (aCGH in 83 gastric adenocarcinomas as a training-sample set. Next, two independent analytical methods were applied to the aCGH data to identify the BAC clones with DNA copy number aberrations that were linked with the disease states. One of the methods, a decision-tree model classifier, identified 6, 4, 4, 4, and 7 clones for estimating lymph node metastasis, liver metastasis, peritoneal dissemination, depth of tumor invasion, and histological type, respectively. In the other method, a clone-by-clone comparison of the frequency of the DNA copy number aberrations selected 26 clones to estimate the disease states. Results By spotting these 50 clones together with 26 frequently or rarely involved clones and 62 reference clones, a mini-array was made to estimate the above parameters, and the diagnostic performance of the mini-array was evaluated for an independent set of 30 gastric cancers (blinded – sample set. In comparison to the clinicopathological features, the overall accuracy was 66.7% for node metastasis, 86.7% for liver metastasis, 86.7% for peritoneal dissemination, and 96.7% for depth of tumor invasion. The intratumoral heterogeneity barely affected the diagnostic performance of the mini-array. Conclusion These results suggest that the mini-array makes it possible to determine an optimal treatment for each of the patients with gastric adenocarcinoma.

  7. CGHregions: Dimension Reduction for Array CGH Data with Minimal Information Loss

    Directory of Open Access Journals (Sweden)

    Mark A. van de Wiel

    2007-01-01

    Full Text Available An algorithm to reduce multi-sample array CGH data from thousands of clones to tens or hundreds of clone regions is introduced. This reduction of the data is performed such that little information is lost, which is possible due to the high dependencies between neighboring clones. The algorithm is explained using a small example. The potential beneficial effects of the algorithm for downstream analysis are illustrated by re-analysis of previously published colorectal cancer data. Using multiple testing corrections suitable for these data, we provide statistical evidence for genomic differences on several clone regions between MSI+ and CIN+ tumors. The algorithm, named CGHregions, is available as an easy-to-use script in R.

  8. A genome-wide analysis of array-based comparative genomic hybridization (CGH) data to detect intra-species variations and evolutionary relationships.

    Science.gov (United States)

    Array-based comparative genomics hybridization (CGH) has gained prevalence as a technique of choice for the detection of structural variations in the genome. In this study, we propose a novel genome-wide method of classification using CGH data, in order to reveal putative phylogenetic relationships ...

  9. Aneuploidy analysis of non-pronuclear embryos from IVF with use of array CGH: a case report.

    Science.gov (United States)

    Lixin, Deng; Zhifeng, Xiang; Cong, He; Jinzhou, Zhang; Hongbin, Xie

    2014-06-01

    By using array comparative genomic hybridization (array CGH), to analyze the aneuploidy of the single blastomeres from non-pronuclear embryos on cleavage-stage in IVF cycle. Four non-pronuclear embryos were got from an IVF cycle, and the each single cell was biopsied from the four cleavage-stage embryos on the third day after the insemination which was investigated by using array CGH. After the biopsy, all the embryos continued to cleave, and lately entered the morula stage on the fifth day, just one embryo 3 was developed to early blastocyst stage on the sixth day. The four blastomere 24 chromosomes showed one X monomer and three normal XY diploids; the autosome chromosomes of blastomeres were abnormally gained or lost at different chromosome from four embryos, such as Embryo 1 : 49,X (-1, -5, -11, -19, -20, -21, -Y, +3, +6, +7, +8, +10, +13, +14, +16, +17, +18); Embryo 2 : 44,XY (-12, -15); Embryo 3: 47,XY (-3, -8, -9, -21, +7, +17, +18, +19, +20); Embryo 4 : 54,XY (+4, +7, +10, +12, +13, +16, +17, +22). With the use of the array CGH, the aneuploidy analysis could review the abnormal chromosomes of single blastomere from the non-pronuclear embryos, which can harbor the risk of abnormal sex chromosome and autosome chromosomes.

  10. A case report of 22q11 deletion syndrome confirmed by array-CGH method

    Directory of Open Access Journals (Sweden)

    Maryam Sedghi

    2012-01-01

    Full Text Available Velo-cardio-facial syndrome (VCFS is caused by a submicroscopic deletion on the long arm of chromosome 22 and affects approximately 1 in 4000 persons, making it the second most prevalent genetic syndrome after Down syndrome and the most common genetic syndrome associated with cleft palate. Most of the 22q11.2 deletion cases are new occurrences or sporadic; however, in about 10 % of families, the deletion is inherited and other family members are affected or at risk for passing this deletion to their children. This report describes a 1.5 years-old male child with clinical signs of velo-cardio-facial syndrome (VCFS presented with heart defect, soft cleft palate, developmental delay, acrocephaly, seizure, MRI abnormalities and descriptive facial feature, such as hypertelorism. Array-CGH test was done to confirm the diagnosis; the result revealed a 2.6 Mbp deletion in 22q11.2 chromosome that containing TBX1 and COMT genes. Our data suggest that haploinsufficiency of TBX1 gene is probably a major contributor to some of the syndrome characteristic signs, such as heart defect. Because of developmental delay and dysmorphic facial feature were observed in the index′s mother and relatives, inherited autosomal dominant form of VCF is probable, and MLPA (multiplex ligation-dependent probe amplification test should be performed for parents to estimate the recurrent risk in next pregnancy.

  11. Different Array CGH profiles within hereditary breast cancer tumors associated to BRCA1 expression and overall survival

    OpenAIRE

    Alvarez, Carolina; Aravena, Andrés; Tapia, Teresa; Rozenblum, Ester; Solís, Luisa; Corvalán, Alejandro; Camus, Mauricio; Alvarez, Manuel; Munroe, David; Maass, Alejandro; Carvallo, Pilar

    2016-01-01

    Background Array CGH analysis of breast tumors has contributed to the identification of different genomic profiles in these tumors. Loss of DNA repair by BRCA1 functional deficiency in breast cancer has been proposed as a relevant contribution to breast cancer progression for tumors with no germline mutation. Identifying the genomic alterations taking place in BRCA1 not expressing tumors will lead us to a better understanding of the cellular functions affected in this heterogeneous disease. M...

  12. Discovery of common Asian copy number variants using integrated high-resolution array CGH and massively parallel DNA sequencing.

    Science.gov (United States)

    Park, Hansoo; Kim, Jong-Il; Ju, Young Seok; Gokcumen, Omer; Mills, Ryan E; Kim, Sheehyun; Lee, Seungbok; Suh, Dongwhan; Hong, Dongwan; Kang, Hyunseok Peter; Yoo, Yun Joo; Shin, Jong-Yeon; Kim, Hyun-Jin; Yavartanoo, Maryam; Chang, Young Wha; Ha, Jung-Sook; Chong, Wilson; Hwang, Ga-Ram; Darvishi, Katayoon; Kim, Hyeran; Yang, Song Ju; Yang, Kap-Seok; Kim, Hyungtae; Hurles, Matthew E; Scherer, Stephen W; Carter, Nigel P; Tyler-Smith, Chris; Lee, Charles; Seo, Jeong-Sun

    2010-05-01

    Copy number variants (CNVs) account for the majority of human genomic diversity in terms of base coverage. Here, we have developed and applied a new method to combine high-resolution array comparative genomic hybridization (CGH) data with whole-genome DNA sequencing data to obtain a comprehensive catalog of common CNVs in Asian individuals. The genomes of 30 individuals from three Asian populations (Korean, Chinese and Japanese) were interrogated with an ultra-high-resolution array CGH platform containing 24 million probes. Whole-genome sequencing data from a reference genome (NA10851, with 28.3x coverage) and two Asian genomes (AK1, with 27.8x coverage and AK2, with 32.0x coverage) were used to transform the relative copy number information obtained from array CGH experiments into absolute copy number values. We discovered 5,177 CNVs, of which 3,547 were putative Asian-specific CNVs. These common CNVs in Asian populations will be a useful resource for subsequent genetic studies in these populations, and the new method of calling absolute CNVs will be essential for applying CNV data to personalized medicine.

  13. High-resolution copy number profiling by array CGH using DNA isolated from formalin-fixed, paraffin-embedded tissues.

    Science.gov (United States)

    van Essen, Hendrik F; Ylstra, Bauke

    2012-01-01

    We describe protocols to acquire high-quality DNA from formalin-fixed, paraffin-embedded (FFPE) tissues for the use in array comparative genome hybridization (CGH). Formalin fixation combined with paraffin embedding is routine procedure for solid malignancies in the diagnostic practice of the pathologist. As a consequence, large archives of FFPE tissues are available in pathology institutes across the globe. This archival material is for many research questions an invaluable resource, with long-term clinical follow-up and survival data available. FFPE is, thus, highly attractive for large genomics studies, including experiments requiring samples for test/learning and validation. Most larger array CGH studies have, therefore, made use of FFPE material and show that CNAs have tumor- and tissue-specific traits (Chin et al. Cancer Cell 10: 529-541, 2006; Fridlyand et al. BMC Cancer 6: 96, 2006; Weiss et al. Oncogene 22: 1872-1879, 2003; Jong et al. Oncogene 26: 1499-1506, 2007). The protocols described are tailored to array CGH of FFPE solid malignancies: from sectioning FFPE blocks to specific cynosures for pathological revisions of sections, DNA isolation, quality testing, and amplification. The protocols are technical in character and elaborate up to the labeling of isolated DNA while further processes and interpretation and data analysis are beyond the scope.

  14. Formalin-fixed paraffin-embedded clinical tissues show spurious copy number changes in array-CGH profiles.

    Science.gov (United States)

    Mc Sherry, E A; Mc Goldrick, A; Kay, E W; Hopkins, A M; Gallagher, W M; Dervan, P A

    2007-11-01

    Formalin-fixed paraffin-embedded (FFPE) archival clinical specimens are invaluable in discovery of prognostic and therapeutic targets for diseases such as cancer. However, the suitability of FFPE-derived genetic material for array-based comparative genomic hybridization (array-CGH) studies is underexplored. In this study, genetic profiles of matched FFPE and fresh-frozen specimens were examined to investigate DNA integrity differences between these sample types and determine the impact this may have on genetic profiles. Genomic DNA was extracted from three patient-matched FFPE and fresh-frozen clinical tissue samples. T47D breast cancer control cells were also grown in culture and processed to yield a fresh T47D sample, a fresh-frozen T47D sample and a FFPE T47D sample. DNA was extracted from all the samples; array-CGH conducted and genetic profiles of matched samples were then compared. A loss of high molecular weight DNA was observed in the FFPE clinical tissues and FFPE T47D samples. A dramatic increase in absolute number of genetic alterations was observed in all FFPE tissues relative to matched fresh-frozen counterparts. In future, alternative fixation and tissue-processing procedures, and/or new DNA extraction and CGH profiling protocols, may be implemented, enabling identification of changes involved in disease progression using stored clinical specimens.

  15. Genome-Wide Screening of Cytogenetic Abnormalities in Multiple Myeloma Patients Using Array-CGH Technique: A Czech Multicenter Experience

    Directory of Open Access Journals (Sweden)

    Jan Smetana

    2014-01-01

    Full Text Available Characteristic recurrent copy number aberrations (CNAs play a key role in multiple myeloma (MM pathogenesis and have important prognostic significance for MM patients. Array-based comparative genomic hybridization (aCGH provides a powerful tool for genome-wide classification of CNAs and thus should be implemented into MM routine diagnostics. We demonstrate the possibility of effective utilization of oligonucleotide-based aCGH in 91 MM patients. Chromosomal aberrations associated with effect on the prognosis of MM were initially evaluated by I-FISH and were found in 93.4% (85/91. Incidence of hyperdiploidy was 49.5% (45/91; del(13(q14 was detected in 57.1% (52/91; gain(1(q21 occurred in 58.2% (53/91; del(17(p13 was observed in 15.4% (14/91; and t(4;14(p16;q32 was found in 18.6% (16/86. Genome-wide screening using Agilent 44K aCGH microarrays revealed copy number alterations in 100% (91/91. Most common deletions were found at 13q (58.9%, 1p (39.6%, and 8p (31.1%, whereas gain of whole 1q was the most often duplicated region (50.6%. Furthermore, frequent homozygous deletions of genes playing important role in myeloma biology such as TRAF3, BIRC1/BIRC2, RB1, or CDKN2C were observed. Taken together, we demonstrated the utilization of aCGH technique in clinical diagnostics as powerful tool for identification of unbalanced genomic abnormalities with prognostic significance for MM patients.

  16. [Accidental finding of a cri du chat syndrome in an adult patient by means of array-CGH].

    Science.gov (United States)

    Ferreirós-Martínez, Raquel; López-Manzanares, Lydia; Alonso-Cerezo, Concepción

    2014-07-16

    Introduccion. El sindrome cri du chat (SCDC) tiene su origen en una delecion parcial o total del brazo corto del cromosoma 5, y es uno de los sindromes de delecion cromosomica mas frecuentes en humanos. La mayoria de los pacientes se diagnostica entre el primer mes y el primer año de vida, si bien aqui se describe el hallazgo de un SCDC en una mujer con sospecha de ataxia espinocerebelar y antecedentes familiares de trastorno bipolar y ataxia, con especial atencion a las caracteristicas clinicas y las tecnicas diagnosticas que permitieron su identificacion. Caso clinico. Mujer de 46 años que presentaba una inteligencia limite, intervenida a los 43 años de faquectomia bilateral. El inicio de la sintomatologia fue durante la infancia, e incluia hipoacusia, ataxia, disartria, disfagia, depresion, deterioro cognitivo y trastorno bipolar. La exploracion fisica revelo microcefalia, micrognatia, pies equinos y ataxia. Se realizo cariotipo y array-CGH en sangre periferica. La paciente presentaba una traslocacion que involucraba los cromosomas 5 y 15, y una inversion del cromosoma 9: 45,XX,inv9(p11q13);t(5,15)(p15.33;q11.2). El array-CGH mostro una delecion de 2,91 Mb en 5p15.33, formula genomica arr 5p15.33 (151537-3057771)x1, que involucraba 20 genes, incluyendo el gen TERT. Conclusiones. La delecion de multiples genes confirmo el diagnostico de SCDC y es la responsable del fenotipo de la paciente. Se pone de manifiesto la importancia de utilizar tecnicas adecuadas de diagnostico (array-CGH, cariotipo en sangre periferica) y la correcta eleccion de estas.

  17. [Middle ear salivary gland choristoma related to branchio-oto-renal syndrome diagnosed by array-CGH].

    Science.gov (United States)

    Amrhein, P; Sittel, C; Spaich, C; Kohlhase, J; Boppert, R; Kohlhof, P; Koitschev, A

    2014-05-01

    Branchio-oto-renal (BOR) syndrome is characterized by ear malformations associated with sensorineural or mixed hearing loss. In addition, preauricular tags, preauricular pits, branchial cleft fistulas and cysts, as well as renal dysplasia are seen. A genetic mutation on chromosome 8, either autosomal dominantly inherited or occuring as a spontaneous mutation, is the cause in the majority of cases. Using array-based comparative genomic hybridization (CGH), it is possible to detect even the smallest genetic changes. Salivary gland choristoma in the middle ear is very rare. Surgical removal and histological clarification are required. PMID:23868653

  18. Array CGH com a primera opció per al diagnòstic genètic postnatal

    OpenAIRE

    Castells Sarret, Neus

    2015-01-01

    La citogenètica convencional detecta un 3-5% dels pacients amb retard global del desenvolupament / discapacitat intel·lectual (RGD / DI) i / o malformacions congènites (MC). L'amplificació de sondes múltiples dependents de lligació (MLPA) permet incrementar la taxa diagnòstica entre 2,4-5,8%. Actualment els arrays d'hibridació genòmica comparada (aCGH) constitueixen l'eina diagnòstica amb major rendiment en pacients amb RGD / DI, MC i trastorns de l'espectre autista (TEA). L'objectiu del p...

  19. Characterization of genomic alterations in radiation-associated breast cancer among childhood cancer survivors, using comparative genomic hybridization (CGH arrays.

    Directory of Open Access Journals (Sweden)

    Xiaohong R Yang

    Full Text Available Ionizing radiation is an established risk factor for breast cancer. Epidemiologic studies of radiation-exposed cohorts have been primarily descriptive; molecular events responsible for the development of radiation-associated breast cancer have not been elucidated. In this study, we used array comparative genomic hybridization (array-CGH to characterize genome-wide copy number changes in breast tumors collected in the Childhood Cancer Survivor Study (CCSS. Array-CGH data were obtained from 32 cases who developed a second primary breast cancer following chest irradiation at early ages for the treatment of their first cancers, mostly Hodgkin lymphoma. The majority of these cases developed breast cancer before age 45 (91%, n = 29, had invasive ductal tumors (81%, n = 26, estrogen receptor (ER-positive staining (68%, n = 19 out of 28, and high proliferation as indicated by high Ki-67 staining (77%, n = 17 out of 22. Genomic regions with low-copy number gains and losses and high-level amplifications were similar to what has been reported in sporadic breast tumors, however, the frequency of amplifications of the 17q12 region containing human epidermal growth factor receptor 2 (HER2 was much higher among CCSS cases (38%, n = 12. Our findings suggest that second primary breast cancers in CCSS were enriched for an "amplifier" genomic subgroup with highly proliferative breast tumors. Future investigation in a larger irradiated cohort will be needed to confirm our findings.

  20. Detection and precise mapping of germline rearrangements in BRCA1, BRCA2, MSH2, and MLH1 using zoom-in array comparative genomic hybridization (aCGH)

    DEFF Research Database (Denmark)

    Staaf, Johan; Törngren, Therese; Rambech, Eva;

    2008-01-01

    of primers for sequence determination of the breakpoints. The array platform can be streamlined for a particular application, e.g., focusing on breast cancer susceptibility genes, with increased capacity using multiformat design, and represents a valuable new tool and complement for genetic screening......Disease-predisposing germline mutations in cancer susceptibility genes may consist of large genomic rearrangements that are challenging to detect and characterize using standard PCR-based mutation screening methods. Here, we describe a custom-made zoom-in microarray comparative genomic...... hybridization (CGH) platform of 60mer oligonucleotides. The 4 x 44 K array format provides high-resolution coverage (200-300 bp) of 400-700 kb genomic regions surrounding six cancer susceptibility genes. We evaluate its performance to accurately detect and precisely map earlier described or novel large germline...

  1. A 91 kb microdeletion at Xq26.2 involving the GPC3 gene in a female fetus with Simpson-Golabi-Behmel syndrome detected by prenatal arrayCGH

    DEFF Research Database (Denmark)

    Ramsing, Mette; Becher, Naja Helene; Christensen, Rikke;

    A 91 kb microdeletion at Xq26.2 involving the GPC3 gene in a female fetus with Simpson-Golabi-Behmel syndrome detected by prenatal arrayCGH......A 91 kb microdeletion at Xq26.2 involving the GPC3 gene in a female fetus with Simpson-Golabi-Behmel syndrome detected by prenatal arrayCGH...

  2. Novel Genomic Aberrations in Testicular Germ Cell Tumors by Array-CGH, and Associated Gene Expression Changes

    Directory of Open Access Journals (Sweden)

    Rolf I. Skotheim

    2006-01-01

    Full Text Available Introduction: Testicular germ cell tumors of adolescent and young adult men (TGCTs generally have near triploid and complex karyotypes. The actual genes driving the tumorigenesis remain essentially to be identified. Materials and Methods: To determine the detailed DNA copy number changes, and investigate their impact on gene expression levels, we performed an integrated microarray profiling of TGCT genomes and transcriptomes. We analyzed 17 TGCTs, three precursor lesions, and the embryonal carcinoma cell lines, NTERA2 and 2102Ep, by comparative genomic hybridization microarrays (array-CGH, and integrated the data with transcriptome profiles of the same samples. Results: The gain of chromosome arm 12p was, as expected, the most common aberration, and we found CCND2, CD9, GAPD, GDF3, NANOG, and TEAD4 to be the therein most highly over-expressed genes. Additional frequent genomic aberrations revealed some shorter chromosomal segments, which are novel to TGCT, as well as known aberrations for which we here refined boundaries. These include gains from 7p15.2 and 21q22.2, and losses of 4p16.3 and 22q13.3. Integration of DNA copy number information to gene expression profiles identified that BRCC3, FOS, MLLT11, NES, and RAC1 may act as novel oncogenes in TGCT. Similarly, DDX26, ERCC5, FZD4, NME4, OPTN, and RB1 were both lost and under-expressed genes, and are thus putative TGCT suppressor genes. Conclusion: This first genome-wide integrated array-CGH and gene expression profiling of TGCT provides novel insights into the genome biology underlying testicular tumorigenesis.

  3. Array comparative genomic hybridisation (aCGH) analysis of premenopausal breast cancers from a nuclear fallout area and matched cases from Western New York

    OpenAIRE

    Varma, G.; Varma, R.; Huang, H.; Pryshchepava, A; Groth, J.; Fleming, D; Nowak, N. J.; McQuaid, D; Conroy, J.; Mahoney, M; Moysich, K.; Falkner, K L; Geradts, J

    2005-01-01

    High-resolution array comparative genomic hybridisation (aCGH) analysis of DNA copy number aberrations (CNAs) was performed on breast carcinomas in premenopausal women from Western New York (WNY) and from Gomel, Belarus, an area exposed to fallout from the 1986 Chernobyl nuclear accident. Genomic DNA was isolated from 47 frozen tumour specimens from 42 patients and hybridised to arrays spotted with more than 3000 BAC clones. In all, 20 samples were from WNY and 27 were from Belarus. In total,...

  4. Selection of single blastocysts for fresh transfer via standard morphology assessment alone and with array CGH for good prognosis IVF patients: results from a randomized pilot study

    Directory of Open Access Journals (Sweden)

    Yang Zhihong

    2012-05-01

    Full Text Available Abstract Background Single embryo transfer (SET remains underutilized as a strategy to reduce multiple gestation risk in IVF, and its overall lower pregnancy rate underscores the need for improved techniques to select one embryo for fresh transfer. This study explored use of comprehensive chromosomal screening by array CGH (aCGH to provide this advantage and improve pregnancy rate from SET. Methods First-time IVF patients with a good prognosis (age Results For patients in Group A (n = 55, 425 blastocysts were biopsied and analyzed via aCGH (7.7 blastocysts/patient. Aneuploidy was detected in 191/425 (44.9% of blastocysts in this group. For patients in Group B (n = 48, 389 blastocysts were microscopically examined (8.1 blastocysts/patient. Clinical pregnancy rate was significantly higher in the morphology + aCGH group compared to the morphology-only group (70.9 and 45.8%, respectively; p = 0.017; ongoing pregnancy rate for Groups A and B were 69.1 vs. 41.7%, respectively (p = 0.009. There were no twin pregnancies. Conclusion Although aCGH followed by frozen embryo transfer has been used to screen at risk embryos (e.g., known parental chromosomal translocation or history of recurrent pregnancy loss, this is the first description of aCGH fully integrated with a clinical IVF program to select single blastocysts for fresh SET in good prognosis patients. The observed aneuploidy rate (44.9% among biopsied blastocysts highlights the inherent imprecision of SET when conventional morphology is used alone. Embryos randomized to the aCGH group implanted with greater efficiency, resulted in clinical pregnancy more often, and yielded a lower miscarriage rate than those selected without aCGH. Additional studies are needed to verify our pilot data and confirm a role for on-site, rapid aCGH for IVF patients contemplating fresh SET.

  5. Genome-wide detection of copy number variations among diverse horse breeds by array CGH.

    Science.gov (United States)

    Wang, Wei; Wang, Shenyuan; Hou, Chenglin; Xing, Yanping; Cao, Junwei; Wu, Kaifeng; Liu, Chunxia; Zhang, Dong; Zhang, Li; Zhang, Yanru; Zhou, Huanmin

    2014-01-01

    Recent studies have found that copy number variations (CNVs) are widespread in human and animal genomes. CNVs are a significant source of genetic variation, and have been shown to be associated with phenotypic diversity. However, the effect of CNVs on genetic variation in horses is not well understood. In the present study, CNVs in 6 different breeds of mare horses, Mongolia horse, Abaga horse, Hequ horse and Kazakh horse (all plateau breeds) and Debao pony and Thoroughbred, were determined using aCGH. In total, seven hundred CNVs were identified ranging in size from 6.1 Kb to 0.57 Mb across all autosomes, with an average size of 43.08 Kb and a median size of 15.11 Kb. By merging overlapping CNVs, we found a total of three hundred and fifty-three CNV regions (CNVRs). The length of the CNVRs ranged from 6.1 Kb to 1.45 Mb with average and median sizes of 38.49 Kb and 13.1 Kb. Collectively, 13.59 Mb of copy number variation was identified among the horses investigated and accounted for approximately 0.61% of the horse genome sequence. Five hundred and eighteen annotated genes were affected by CNVs, which corresponded to about 2.26% of all horse genes. Through the gene ontology (GO), genetic pathway analysis and comparison of CNV genes among different breeds, we found evidence that CNVs involving 7 genes may be related to the adaptation to severe environment of these plateau horses. This study is the first report of copy number variations in Chinese horses, which indicates that CNVs are ubiquitous in the horse genome and influence many biological processes of the horse. These results will be helpful not only in mapping the horse whole-genome CNVs, but also to further research for the adaption to the high altitude severe environment for plateau horses.

  6. Combined subtractive cDNA cloning and array CGH: an efficient approach for identification of overexpressed genes in DNA amplicons

    Directory of Open Access Journals (Sweden)

    De Paepe Anne

    2004-02-01

    Full Text Available Abstract Background Activation of proto-oncogenes by DNA amplification is an important mechanism in the development and maintenance of cancer cells. Until recently, identification of the targeted genes relied on labour intensive and time consuming positional cloning methods. In this study, we outline a straightforward and efficient strategy for fast and comprehensive cloning of amplified and overexpressed genes. Results As a proof of principle, we analyzed neuroblastoma cell line IMR-32, with at least two amplification sites along the short arm of chromosome 2. In a first step, overexpressed cDNA clones were isolated using a PCR based subtractive cloning method. Subsequent deposition of these clones on a custom microarray and hybridization with IMR-32 DNA, resulted in the identification of clones that were overexpressed due to gene amplification. Using this approach, amplification of all previously reported amplified genes in this cell line was detected. Furthermore, four additional clones were found to be amplified, including the TEM8 gene on 2p13.3, two anonymous transcripts, and a fusion transcript, resulting from 2p13.3 and 2p24.3 fused sequences. Conclusions The combinatorial strategy of subtractive cDNA cloning and array CGH analysis allows comprehensive amplicon dissection, which opens perspectives for improved identification of hitherto unknown targeted oncogenes in cancer cells.

  7. Analysis of myelodysplastic syndromes with complex karyotypes by high-resolution comparative genomic hybridization and subtelomeric CGH array

    NARCIS (Netherlands)

    Martinez-Ramirez, A; Urioste, M; Melchor, L; Blesa, D; Valle, L; de Andres, SA; Kok, K; Calasanz, MJ; Cigudosa, JC; Benitez, J

    2005-01-01

    Molecular cytogenetic techniques enabled us to clarify numerical and structural alterations previously detected by conventional cytogenetic techniques in 37 patients who had myelodysplastic syndromes with complex karyotypes. Using high-resolution comparative genomic hybridization (HR-CGH), we found

  8. [Subchromosomal microdeletion identified by molecular karyotyping using DNA microarrays (array CGH) in Rett syndrome girls negative for MECP2 gene mutations].

    Science.gov (United States)

    Vorsanova, S G; Iurov, I Iu; Voinova, V Iu; Kurinnaia, O S; Zelenova, M A; Demidova, I A; Ulas, E V; Iurov, Iu B

    2013-01-01

    Molecular karyotyping using DNA microarrays (array CGH) was applied for identification of subchromosomal microdeletions in a cohort of 12 girls with clinical features of RETT syndrome, but negative for MECP2 gene mutations. Recurrent microdeletions of MECP2 gene in chromosome X (locus Xq28) were identified in 5 girls of 12 studied. Probably RTT girls with subchromosomic microdeletions in Xq28 could represent a special subtype of the disease, which appears as clinically milder than the classic form of disease. In one case, an atypical form of RTT was associated with genomic abnormalities affecting CDKL5 gene and region critical for microdeletion Prader-Willi and Angelman syndromes (15q11.2). In addition, data are presented for the first time that genetic variation in regions 3p13, 3q27.1, and 1q21.1-1q21.2 could associate with RTT-like clinical manifestations. Without application of molecular karyotyping technology and bioinformatic method of assessing the pathogenic significance of genomic rearrangements these RTT-like girls negative for MECP2 gene mutations were considered as cases of idiopathic mental retardation associated with autism. It should be noted that absence of intragenic mutations in MECP2 gene is not sufficient criteria to reject the clinical diagnosis of RTT. To avoid errors in the genetic diagnosis of this genetically heterogeneous brain disease molecular cytogenetic studies using high resolution oligonucleotide array CGH (molecular karyotyping) are needed.

  9. Array CGH identifies distinct DNA copy number profiles of oncogenes and tumor suppressor genes in chromosomal- and microsatellite-unstable sporadic colorectal carcinomas.

    Science.gov (United States)

    Lassmann, Silke; Weis, Roland; Makowiec, Frank; Roth, Jasmine; Danciu, Mihai; Hopt, Ulrich; Werner, Martin

    2007-03-01

    DNA copy number changes represent molecular fingerprints of solid tumors and are as such relevant for better understanding of tumor development and progression. In this study, we applied genome-wide array comparative genomic hybridization (aCGH) to identify gene-specific DNA copy number changes in chromosomal (CIN)- and microsatellite (MIN)-unstable sporadic colorectal cancers (sCRC). Genomic DNA was extracted from microdissected, matching normal colorectal epithelium and invasive tumor cells of formalin-fixed and paraffin-embedded tissues of 22 cases with colorectal cancer (CIN = 11, MIN = 11). DNA copy number changes were determined by aCGH for 287 target sequences in tumor cell DNAs, using pooled normal DNAs as reference. aCGH data of tumor cell DNAs was confirmed by fluorescence in situ hybridization (FISH) for three genes on serial tissues as those used for aCGH. aCGH revealed DNA copy number changes previously described by metaphase CGH (gains 7, 8q, 13q, and 20q; losses 8p, 15q, 18q, and 17p). However, chromosomal regions 20q, 13q, 7, and 17p were preferentially altered in CIN-type tumors and included DNA amplifications of eight genes on chromosome 20q (TOP1, AIB1, MYBL2, CAS, PTPN1, STK15, ZNF217, and CYP24), two genes on chromosome 13q (BRCA2 and D13S25), and three genes on chromosome 7 (IL6, CYLN2, and MET) as well as DNA deletions of two genes on chromosome 17p (HIC1 and LLGL1). Finally, additional CIN-tumor-associated DNA amplifications were identified for EXT1 (8q24.11) and MYC (8q24.12) as well as DNA deletions for MAP2K5 (15q23) and LAMA3 (18q11.2). In contrast, distinct MIN-tumor-associated DNA amplifications were detected for E2F5 (8p22-q21.3), GARP (11q13.5-q14), ATM (11q22.3), KAL (Xp22.3), and XIST (Xq13.2) as well as DNA deletions for RAF1 (3p25), DCC (18q21.3), and KEN (21q tel). aCGH revealed distinct DNA copy number changes of oncogenes and tumor suppressor genes in CIN- and MIN-type sporadic colorectal carcinomas. The identified candidate

  10. Random DNA fragmentation allows detection of single-copy, single-exon alterations of copy number by oligonucleotide array CGH in clinical FFPE samples.

    Science.gov (United States)

    Hostetter, Galen; Kim, Su Young; Savage, Stephanie; Gooden, Gerald C; Barrett, Michael; Zhang, Jian; Alla, Lalitamba; Watanabe, April; Einspahr, Janine; Prasad, Anil; Nickoloff, Brian J; Carpten, John; Trent, Jeffrey; Alberts, David; Bittner, Michael

    2010-01-01

    Genomic technologies, such as array comparative genomic hybridization (aCGH), increasingly offer definitive gene dosage profiles in clinical samples. Historically, copy number profiling was limited to large fresh-frozen tumors where intact DNA could be readily extracted. Genomic analyses of pre-neoplastic tumors and diagnostic biopsies are often limited to DNA processed by formalin-fixation and paraffin-embedding (FFPE). We present specialized protocols for DNA extraction and processing from FFPE tissues utilizing DNase processing to generate randomly fragmented DNA. The protocols are applied to FFPE clinical samples of varied tumor types, from multiple institutions and of varied block age. Direct comparative analyses with regression coefficient were calculated on split-sample (portion fresh/portion FFPE) of colorectal tumor samples. We show equal detection of a homozygous loss of SMAD4 at the exon-level in the SW480 cell line and gene-specific alterations in the split tumor samples. aCGH application to a set of archival FFPE samples of skin squamous cell carcinomas detected a novel hemizygous deletion in INPP5A on 10q26.3. Finally we present data on derivative of log ratio, a particular sensitive detector of measurement variance, for 216 sequential hybridizations to assess protocol reliability over a wide range of FFPE samples.

  11. Custom CGH array profiling of copy number variations (CNVs on chromosome 6p21.32 (HLA locus in patients with venous malformations associated with multiple sclerosis

    Directory of Open Access Journals (Sweden)

    Salvi Fabrizio

    2010-04-01

    Full Text Available Abstract Background Multiple sclerosis (MS is a complex disorder thought to result from an interaction between environmental and genetic predisposing factors which have not yet been characterised, although it is known to be associated with the HLA region on 6p21.32. Recently, a picture of chronic cerebrospinal venous insufficiency (CCSVI, consequent to stenosing venous malformation of the main extra-cranial outflow routes (VM, has been described in patients affected with MS, introducing an additional phenotype with possible pathogenic significance. Methods In order to explore the presence of copy number variations (CNVs within the HLA locus, a custom CGH array was designed to cover 7 Mb of the HLA locus region (6,899,999 bp; chr6:29,900,001-36,800,000. Genomic DNA of the 15 patients with CCSVI/VM and MS was hybridised in duplicate. Results In total, 322 CNVs, of which 225 were extragenic and 97 intragenic, were identified in 15 patients. 234 known polymorphic CNVs were detected, the majority of these being situated in non-coding or extragenic regions. The overall number of CNVs (both extra- and intragenic showed a robust and significant correlation with the number of stenosing VMs (Spearman: r = 0.6590, p = 0.0104; linear regression analysis r = 0.6577, p = 0.0106. The region we analysed contains 211 known genes. By using pathway analysis focused on angiogenesis and venous development, MS, and immunity, we tentatively highlight several genes as possible susceptibility factor candidates involved in this peculiar phenotype. Conclusions The CNVs contained in the HLA locus region in patients with the novel phenotype of CCSVI/VM and MS were mapped in detail, demonstrating a significant correlation between the number of known CNVs found in the HLA region and the number of CCSVI-VMs identified in patients. Pathway analysis revealed common routes of interaction of several of the genes involved in angiogenesis and immunity contained within this region

  12. Tiling array-CGH for the assessment of genomic similarities among synchronous unilateral and bilateral invasive breast cancer tumor pairs

    Directory of Open Access Journals (Sweden)

    Ringnér Markus

    2008-07-01

    Full Text Available Abstract Background Today, no objective criteria exist to differentiate between individual primary tumors and intra- or intermammary dissemination respectively, in patients diagnosed with two or more synchronous breast cancers. To elucidate whether these tumors most likely arise through clonal expansion, or whether they represent individual primary tumors is of tumor biological interest and may have clinical implications. In this respect, high resolution genomic profiling may provide a more reliable approach than conventional histopathological and tumor biological factors. Methods 32 K tiling microarray-based comparative genomic hybridization (aCGH was used to explore the genomic similarities among synchronous unilateral and bilateral invasive breast cancer tumor pairs, and was compared with histopathological and tumor biological parameters. Results Based on global copy number profiles and unsupervised hierarchical clustering, five of ten (p = 1.9 × 10-5 unilateral tumor pairs displayed similar genomic profiles within the pair, while only one of eight bilateral tumor pairs (p = 0.29 displayed pair-wise genomic similarities. DNA index, histological type and presence of vessel invasion correlated with the genomic analyses. Conclusion Synchronous unilateral tumor pairs are often genomically similar, while synchronous bilateral tumors most often represent individual primary tumors. However, two independent unilateral primary tumors can develop synchronously and contralateral tumor spread can occur. The presence of an intraductal component is not informative when establishing the independence of two tumors, while vessel invasion, the presence of which was found in clustering tumor pairs but not in tumor pairs that did not cluster together, supports the clustering outcome. Our data suggest that genomically similar unilateral tumor pairs may represent a more aggressive disease that requires the addition of more severe treatment modalities, and

  13. Array comparative genomic hybridisation (aCGH) analysis of premenopausal breast cancers from a nuclear fallout area and matched cases from Western New York.

    Science.gov (United States)

    Varma, G; Varma, R; Huang, H; Pryshchepava, A; Groth, J; Fleming, D; Nowak, N J; McQuaid, D; Conroy, J; Mahoney, M; Moysich, K; Falkner, K L; Geradts, J

    2005-09-19

    High-resolution array comparative genomic hybridisation (aCGH) analysis of DNA copy number aberrations (CNAs) was performed on breast carcinomas in premenopausal women from Western New York (WNY) and from Gomel, Belarus, an area exposed to fallout from the 1986 Chernobyl nuclear accident. Genomic DNA was isolated from 47 frozen tumour specimens from 42 patients and hybridised to arrays spotted with more than 3000 BAC clones. In all, 20 samples were from WNY and 27 were from Belarus. In total, 34 samples were primary tumours and 13 were lymph node metastases, including five matched pairs from Gomel. The average number of total CNAs per sample was 76 (range 35-134). We identified 152 CNAs (92 gains and 60 losses) occurring in more than 10% of the samples. The most common amplifications included gains at 8q13.2 (49%), at 1p21.1 (36%), and at 8q24.21 (36%). The most common deletions were at 1p36.22 (26%), at 17p13.2 (26%), and at 8p23.3 (23%). Belarussian tumours had more amplifications and fewer deletions than WNY breast cancers. HER2/neu negativity and younger age were also associated with a higher number of gains and fewer losses. In the five paired samples, we observed more discordant than concordant DNA changes. Unsupervised hierarchical cluster analysis revealed two distinct groups of tumours: one comprised predominantly of Belarussian carcinomas and the other largely consisting of WNY cases. In total, 50 CNAs occurred significantly more commonly in one cohort vs the other, and these included some candidate signature amplifications in the breast cancers in women exposed to significant radiation. In conclusion, our high-density aCGH study has revealed a large number of genetic aberrations in individual premenopausal breast cancer specimens, some of which had not been reported before. We identified a distinct CNA profile for carcinomas from a nuclear fallout area, suggesting a possible molecular fingerprint of radiation-associated breast cancer. PMID:16222315

  14. An Xq22.3 duplication detected by comparative genomic hybridization microarray (Array-CGH) defines a new locus (FGS5) for FG syndrome.

    Science.gov (United States)

    Jehee, Fernanda Sarquis; Rosenberg, Carla; Krepischi-Santos, Ana Cristina; Kok, Fernando; Knijnenburg, Jeroen; Froyen, Guy; Vianna-Morgante, Angela M; Opitz, John M; Passos-Bueno, Maria Rita

    2005-12-15

    FG syndrome is an X-linked multiple congenital anomalies (MCA) syndrome. It has been mapped to four distinct loci FGS1-4, through linkage analysis (Xq13, Xp22.3, and Xp11.4-p11.3) and based on the breakpoints of an X chromosome inversion (Xq11:Xq28), but so far no gene has been identified. We describe a boy with FG syndrome who has an inherited duplication at band Xq22.3 detected by comparative genomic hybridization microarray (Array-CGH). These duplication maps outside all four loci described so far for FG syndrome, representing therefore a new locus, which we propose to be called FGS5. MID2, a gene closely related to MID1, which is known to be mutated in Opitz G/BBB syndrome, maps within the duplicated segment of our patient. Since FG and Opitz G/BBB syndromes share many manifestations we considered MID2 a candidate gene for FG syndrome. We also discuss the involvement of other potential genes within the duplicated segment and its relationship with clinical symptoms of our patient, as well as the laboratory abnormalities found in his mother, a carrier of the duplication.

  15. Identification by full-coverage array CGH of human DNA copy number increases relative to chimpanzee and gorilla

    OpenAIRE

    Wilson, Gary M.; Flibotte, Stephane; Missirlis, Perseus I.; Marra, Marco A.; Jones, Steven; Thornton, Kevin; Clark, Andrew G.; Holt, Robert A.

    2006-01-01

    Duplication of chromosomal segments and associated genes is thought to be a primary mechanism for generating evolutionary novelty. By comparative genome hybridization using a full-coverage (tiling) human BAC array with 79-kb resolution, we have identified 63 chromosomal segments, ranging in size from 0.65 to 1.3 Mb, that have inferred copy number increases in human relative to chimpanzee. These segments span 192 Ensembl genes, including 82 gene duplicates (41 reciprocal best BLAST matches). S...

  16. Age dependence of tumor genetics in unfavorable neuroblastoma: arrayCGH profiles of 34 consecutive cases, using a Swedish 25-year neuroblastoma cohort for validation

    International Nuclear Information System (INIS)

    Aggressive neuroblastoma remains a significant cause of childhood cancer death despite current intensive multimodal treatment protocols. The purpose of the present work was to characterize the genetic and clinical diversity of such tumors by high resolution arrayCGH profiling. Based on a 32K BAC whole-genome tiling path array and using 50-250K Affymetrix SNP array platforms for verification, DNA copy number profiles were generated for 34 consecutive high-risk or lethal outcome neuroblastomas. In addition, age and MYCN amplification (MNA) status were retrieved for 112 unfavorable neuroblastomas of the Swedish Childhood Cancer Registry, representing a 25-year neuroblastoma cohort of Sweden, here used for validation of the findings. Statistical tests used were: Fisher’s exact test, Bayes moderated t-test, independent samples t-test, and correlation analysis. MNA or segmental 11q loss (11q-) was found in 28/34 tumors. With two exceptions, these aberrations were mutually exclusive. Children with MNA tumors were diagnosed at significantly younger ages than those with 11q- tumors (mean: 27.4 vs. 69.5 months; p=0.008; n=14/12), and MNA tumors had significantly fewer segmental chromosomal aberrations (mean: 5.5 vs. 12.0; p<0.001). Furthermore, in the 11q- tumor group a positive correlation was seen between the number of segmental aberrations and the age at diagnosis (Pearson Correlation 0.606; p=0.037). Among nonMNA/non11q- tumors (n=6), one tumor displayed amplicons on 11q and 12q and three others bore evidence of progression from low-risk tumors due to retrospective evidence of disease six years before diagnosis, or due to tumor profiles with high proportions of numerical chromosomal aberrations. An early age at diagnosis of MNA neuroblastomas was verified by registry data, with an average of 29.2 months for 43 cases that were not included in the present study. MNA and segmental 11q loss define two major genetic variants of unfavorable neuroblastoma with apparent

  17. High-resolution array CGH profiling identifies Na/K transporting ATPase interacting 2 (NKAIN2 as a predisposing candidate gene in neuroblastoma.

    Directory of Open Access Journals (Sweden)

    Paolo Romania

    Full Text Available Neuroblastoma (NB, the most common solid cancer in early childhood, usually occurs sporadically but also its familial occurance is known in 1-2% of NB patients. Germline mutations in the ALK and PHOX2B genes have been found in a subset of familial NBs. However, because some individuals harbouring mutations in these genes do not develop this tumor, additional genetic alterations appear to be required for NB pathogenesis. Herein, we studied an Italian family with three NB patients, two siblings and a first cousin, carrying an ALK germline-activating mutation R1192P, that was inherited from their unaffected mothers and with no mutations in the PHOX2B gene. A comparison between somatic and germline DNA copy number changes in the two affected siblings by a high resolution array-based Comparative Genomic Hybridization (CGH analysis revealed a germline gain at NKAIN2 (Na/K transporting ATPase interacting 2 locus in one of the sibling, that was inherited from the parent who does not carry the ALK mutation. Surprisingly, NKAIN2 was expressed at high levels also in the affected sibling that lacks the genomic gain at this locus, clearly suggesting the existance of other regulatory mechanisms. High levels of NKAIN2 were detected in the MYCN-amplified NB cell lines and in the most aggressive NB lesions as well as in the peripheral blood of a large cohort of NB patients. Consistent with a role of NKAIN2 in NB development, NKAIN2 was down-regulated during all-trans retinoic acid differentiation in two NB cell lines. Taken together, these data indicate a potential role of NKAIN2 gene in NB growth and differentiation.

  18. Use Array-basedCGH technology for fetal chromosomal anomalies syndrome diagnosis%运用Array-basedCGH技术进行胎儿染色体异常综合征的诊断研究

    Institute of Scientific and Technical Information of China (English)

    易广才; 童华

    2011-01-01

    Objective: To use high - resolution Array - basedCCH (aCCH) technology for chromosomes of the small mutation (missing or amplified) to cause fetal malformation syndrome, and its formation mechanism. Method:use of a Agilent4 × 44K CGH chip to detect the normal samples and specimens from the three malformation of Fetal DNA ( M1:normalfetal; M2:abdominal wall defect fetal; M3: single umbilical artery with congenital heart disease, double outlet right ventricle, Ventricular septal defect and mitral atresia pulmonary artery stenosis; M4: karyorype 47, +21, congenital heart disease, Ventricular septal defect, fetus side lateral ventricle broadening, the fetus is small) by the comparative genomic hybridization. Results: M2/MI; high copy (amplification) 560 of fragments of DNA (genes), low copy or missing 1504 of DNA (genes) of the fragment; M3/M1: high copy 511 fragments of DNA (genes) , low copy or missing 1142 articles DNA (genes) of the fragment; M4/M1: section 3034 high copy DNA (genes) of the fragment, low copy or missing section 3571 DNA (genes) fragment. Conclusion; the small mutation of chromosome fragments is one of the main causes which lead to fetal malformation syndrome, the high - resolution aCCH technology is able to be used for quickly and accurately on its testing and prenatal diagnosis.%目的,运用高分辨率的Array-basedCGH(aCGH)技术研究染色体的微小变异(缺失或扩增)引起的胎儿畸形综合征,及其形成机理.方法 采用Agilent4×44K CGH,芯片对一个正常标本和三个畸形胎儿标本DNA(MI 正常胎儿;M2腹壁缺损;M3单脐动脉合并先天性心脏病,右室双出口,空间隔缺损,肺动脉狭窄,二尖瓣闭锁;M4染色体核型47,+21,先天性心脏病,室间隔缺损,胎儿一侧脑室增宽,胎儿偏小)进行比较基因组杂交捡洲.结果 M2/Ml:高拷贝(扩增)560条DNA(基因)片段,低拷贝或缺失1504条DNA(基因)片断;M3/M1:高拷贝511条DNA(基因)片段,低拷贝或缺失1142

  19. Prenatal diagnosis of chromosomal abnormalities using array-based comparative genomic hybridization

    Science.gov (United States)

    This study was designed to evaluate the feasibility of using a targeted array-CGH strategy for prenatal diagnosis of genomic imbalances in a clinical setting of current pregnancies. Women undergoing prenatal diagnosis were counseled and offered array-CGH (BCM V4.0) in addition to routine chromosome ...

  20. Delineation of a de novo 7q21.3q31.1 Deletion by CGH-SNP Arrays in a Girl with Multiple Congenital Anomalies Including Severe Glaucoma

    OpenAIRE

    Martínez-Jacobo, L.; Córdova-Fletes, C.; Ortiz-López, R.; Rivas, F; Saucedo-Carrasco, C.; Rojas-Martínez, A.

    2013-01-01

    In this study, we present a female patient with a constitutional de novo deletion in 7q21.3q31.1 as determined by G-banding and CGH-SNP arrays. She exhibited, among other features, psychomotor retardation, congenital severe bilateral glaucoma, a cleft palate, and heart defect. Microarray assay disclosed a deleted 12.5-Mb region roughly 88 kb downstream the ectrodactyly critical region; thus, the patient's final karyotype was 46,XX.arr 7q21.3q31.1(96,742,140-109,246,085)×1 dn. This girl repres...

  1. Development of a novel ozone- and photo-stable HyPer5 red fluorescent dye for array CGH and microarray gene expression analysis with consistent performance irrespective of environmental conditions

    Directory of Open Access Journals (Sweden)

    Kille Peter

    2008-11-01

    Full Text Available Abstract Background Array-based comparative genomic hybridization (CGH and gene expression profiling have become vital techniques for identifying molecular defects underlying genetic diseases. Regardless of the microarray platform, cyanine dyes (Cy3 and Cy5 are one of the most widely used fluorescent dye pairs for microarray analysis owing to their brightness and ease of incorporation, enabling high level of assay sensitivity. However, combining both dyes on arrays can become problematic during summer months when ozone levels rise to near 25 parts per billion (ppb. Under such conditions, Cy5 is known to rapidly degrade leading to loss of signal from either "homebrew" or commercial arrays. Cy5 can also suffer disproportionately from dye photobleaching resulting in distortion of (Cy5/Cy3 ratios used in copy number analysis. Our laboratory has been active in fluorescent dye research to find a suitable alternative to Cy5 that is stable to ozone and resistant to photo-bleaching. Here, we report on the development of such a dye, called HyPer5, and describe its' exceptional ozone and photostable properties on microarrays. Results Our results show HyPer5 signal to be stable to high ozone levels. Repeated exposure of mouse arrays hybridized with HyPer5-labeled cDNA to 300 ppb ozone at 5, 10 and 15 minute intervals resulted in no signal loss from the dye. In comparison, Cy5 arrays showed a dramatic 80% decrease in total signal during the same interval. Photobleaching experiments show HyPer5 to be resistant to light induced damage with 3- fold improvement in dye stability over Cy5. In high resolution array CGH experiments, HyPer5 is demonstrated to detect chromosomal aberrations at loci 2p21-16.3 and 15q26.3-26.2 from three patient sample using bacterial artificial chromosome (BAC arrays. The photostability of HyPer5 is further documented by repeat array scanning without loss of detection. Additionally, HyPer5 arrays are shown to preserve sensitivity and

  2. Characterization of genetic rearrangements in esophageal squamous carcinoma cell lines by a combination of M-FISH and array-CGH: further confirmation of some split genomic regions in primary tumors

    International Nuclear Information System (INIS)

    Chromosomal and genomic aberrations are common features of human cancers. However, chromosomal numerical and structural aberrations, breakpoints and disrupted genes have yet to be identified in esophageal squamous cell carcinoma (ESCC). Using multiplex-fluorescence in situ hybridization (M-FISH) and oligo array-based comparative hybridization (array-CGH), we identified aberrations and breakpoints in six ESCC cell lines. Furthermore, we detected recurrent breakpoints in primary tumors by dual-color FISH. M-FISH and array-CGH results revealed complex numerical and structural aberrations. Frequent gains occurred at 3q26.33-qter, 5p14.1-p11, 7pter-p12.3, 8q24.13-q24.21, 9q31.1-qter, 11p13-p11, 11q11-q13.4, 17q23.3-qter, 18pter-p11, 19 and 20q13.32-qter. Losses were frequent at 18q21.1-qter. Breakpoints that clustered within 1 or 2 Mb were identified, including 9p21.3, 11q13.3-q13.4, 15q25.3 and 3q28. By dual-color FISH, we observed that several recurrent breakpoint regions in cell lines were also present in ESCC tumors. In particular, breakpoints clustered at 11q13.3-q13.4 were identified in 43.3% (58/134) of ESCC tumors. Both 11q13.3-q13.4 splitting and amplification were significantly correlated with lymph node metastasis (LNM) (P = 0.004 and 0.022) and advanced stages (P = 0.004 and 0.039). Multivariate logistic regression analysis revealed that only 11q13.3-q13.4 splitting was an independent predictor for LNM (P = 0.026). The combination of M-FISH and array-CGH helps produce more accurate karyotypes. Our data provide significant, detailed information for appropriate uses of these ESCC cell lines for cytogenetic and molecular biological studies. The aberrations and breakpoints detected in both the cell lines and primary tumors will contribute to identify affected genes involved in the development and progression of ESCC

  3. A male newborn with VACTERL association and Fanconi anemia with a FANCB deletion detected by array comparative genomic hybridization (aCGH).

    Science.gov (United States)

    Umaña, Luis A; Magoulas, Pilar; Bi, Weimin; Bacino, Carlos A

    2011-12-01

    We report on a male newborn with multiple congenital abnormalities consistent with the diagnosis of VACTERL association (vertebral, anal, cardiac, tracheo-esophageal fistula, renal, and limb anomalies), who had Fanconi anemia (complementation group B) recognized by the detection of a deletion in chromosome Xp22.2 using an oligonucleotide array. The diagnosis of Fanconi anemia was confirmed by increased chromosomal breakage abnormalities observed in cultured cells that were treated with cross-linking agents. This is the first report in the literature of Fanconi anemia complementation group B detected by oligonucleotide array testing postnatally.

  4. Delineation of a de novo 7q21.3q31.1 Deletion by CGH-SNP Arrays in a Girl with Multiple Congenital Anomalies Including Severe Glaucoma.

    Science.gov (United States)

    Martínez-Jacobo, L; Córdova-Fletes, C; Ortiz-López, R; Rivas, F; Saucedo-Carrasco, C; Rojas-Martínez, A

    2013-09-01

    In this study, we present a female patient with a constitutional de novo deletion in 7q21.3q31.1 as determined by G-banding and CGH-SNP arrays. She exhibited, among other features, psychomotor retardation, congenital severe bilateral glaucoma, a cleft palate, and heart defect. Microarray assay disclosed a deleted 12.5-Mb region roughly 88 kb downstream the ectrodactyly critical region; thus, the patient's final karyotype was 46,XX.arr 7q21.3q31.1(96,742,140-109,246,085)×1 dn. This girl represents the fourth patient described so far with congenital glaucoma and a deletion encompassing or overlapping the 7q21.3q31.1 region, and confirms the presence of a locus or loci related to such a clinical feature. According to our results, the proneness to ocular defects secondary to 7q intermediate deletions could be caused by co-deletion of TAC1, HBP1, and a small cluster of cytochrome P450 genes (subfamily 3A). This conclusion is supported by their functional roles and expression locations as well as because TAC1 is related to the functional pathway of the MYOC gene whose mutations are linked to glaucoma. Moreover, given that this girl is clinically reminiscent of several phenotypes related to diverse deletions within 7q21q32, our results and observations offer a general overview of the gene content of deletions/phenotypes overlapping 7q21.3q31.1 and confirm that loci distal to DLX genes including the CUX1 gene and potential regulatory elements downstream from DLX5 are unrelated to ectrodactyly. PMID:24167464

  5. arrayCGHbase: an analysis platform for comparative genomic hybridization microarrays

    Directory of Open Access Journals (Sweden)

    Moreau Yves

    2005-05-01

    Full Text Available Abstract Background The availability of the human genome sequence as well as the large number of physically accessible oligonucleotides, cDNA, and BAC clones across the entire genome has triggered and accelerated the use of several platforms for analysis of DNA copy number changes, amongst others microarray comparative genomic hybridization (arrayCGH. One of the challenges inherent to this new technology is the management and analysis of large numbers of data points generated in each individual experiment. Results We have developed arrayCGHbase, a comprehensive analysis platform for arrayCGH experiments consisting of a MIAME (Minimal Information About a Microarray Experiment supportive database using MySQL underlying a data mining web tool, to store, analyze, interpret, compare, and visualize arrayCGH results in a uniform and user-friendly format. Following its flexible design, arrayCGHbase is compatible with all existing and forthcoming arrayCGH platforms. Data can be exported in a multitude of formats, including BED files to map copy number information on the genome using the Ensembl or UCSC genome browser. Conclusion ArrayCGHbase is a web based and platform independent arrayCGH data analysis tool, that allows users to access the analysis suite through the internet or a local intranet after installation on a private server. ArrayCGHbase is available at http://medgen.ugent.be/arrayCGHbase/.

  6. SEPS solar array design and technology evaluation

    Science.gov (United States)

    Elms, R. V., Jr.; Young, L. E.

    1975-01-01

    The technology developments required and a preliminary design of a lightweight 25 kW solar array for the solar electric propulsion stage (SEPS) have been defined. The requirements for a 65 W/Kg SEPS solar array system requires significant component weight reductions over present state-of-the-art flexible solar arrays in both electrical and structural-mechanical designs. A requirement for operation from 0.3 au to 6.0 au presents a wide range of temperature environments as well as severe combined thermal/vacuum/UV radiation environments. Additional requirements are capability for partial array retraction operation, and capability for full retraction and automatic preloading for survival of the Shuttle reentry environment. An assessment of current lightweight flexible solar array technology is made against the SEPS solar array requirements and new technology requirements are defined. A preliminary design and the operating characteristics of a flat-fold solar array system meeting the SEPS requirements is presented. A full-width, 10-ft-tall functional array model, including representative welded electrical modules and a model astromast, was fabricated and tested.

  7. OPTIMAL DESIGN OF SMART ANTENNA ARRAY

    Institute of Scientific and Technical Information of China (English)

    Gao Feng; Liu Qizhong; Shan Runhong; Zhang Hou

    2004-01-01

    This letter investigates an efficient design procedure integrating the Genetic Algorithm (GA) with the Finite Difference Time Domain (FDTD) for the fast optimal design of Smart Antenna Arrays (SAA). The FDTD is used to analyze SAA with mutual coupling. Then,on the basis of the Maximal Signal to Noise Ratio (MSNR) criteria, the GA is applied to the optimization of weighting elements and structure of SAA. Finally, the effectiveness of the analysis is evaluated by experimental antenna arrays.

  8. Sentinel-3 Solar Array Design

    Science.gov (United States)

    Combet, Y.; Reutenauer, X.; Mouret, G.; Guerrere, S.; Ergan, A.; Ferrando, E.; Riva, S.; Hodgetts, P.; Levesque, D.; D'Accolti, G.

    2011-10-01

    Sentinel-3 is primarily a mission to support services relating to the marine environment, with capability to serve numerous land-, atmospheric- and cryospheric- based application areas. The mission's main objective is to determine parameters, such as sea-surface topography, sea- and land-surface temperatures, as well as ocean- and land-surface colours with high-end accuracy and reliability. For this mission, Thales Alenia Space has been selected as the spacecraft prime contractor and is also responsible for the solar array. In this frame, TAS leads a European industrial team, comprising Selex Galileo for the photovoltaic assembly and Patria for the panel substrate.

  9. 2013 CGH Awardees

    Science.gov (United States)

    The National cancer institute, CENTER FOR GLOBAL HEALTH, in collaboration with the OFFICE OF CANCER CENTERS, is pleased to announce the 2013 awardees of the Request for Proposals for Pilot Collaborations with Low- and Mid-Income Countries (LMICs) in Global Cancer Research or Global Health Research at NCI-Designated Cancer Centers.  In 2013, the Center for Global Health and the Office of Cancer Centers developed a funding opportunity to promote research collaborations between NCI-Designated Cancer Centers with institutions in LMICs.

  10. Arrayed-Waveguide Layout for AWG Design

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The key issue of the complex geometry for arrayed-waveguide grating(AWG)is the design of length difference.Conventional-type scheme and improved-type scheme of the ar-rayed -waveguide layout are proposed.For each scheme,the layout rules were given and one ex-ample was presented. At last ,the two schemes were compared with each other.

  11. Validation of multiplex ligation-dependent probe amplification for confirmation of array comparative genomic hybridization.

    Science.gov (United States)

    Jennings, Lawrence J; Yu, Min; Fitzpatrick, Carrie; Smith, Frederick A

    2011-09-01

    The American College of Medical Genetics recommends that each laboratory should confirm abnormal or ambiguous results detected by array comparative genomic hybridization (aCGH). At present, the gold standard method for aCGH confirmation is fluorescent in situ hybridization (FISH). However, FISH is not well suited for small tandem duplications or very small deletions that are detectable by oligonucleotide arrays. Therefore, we developed and validated multiplex ligation-dependent probe amplification (MLPA) for aCGH confirmation. The method performance validation showed linearity through the expected analytical measurement range (0.05 to 2 genome equivalents). The interassay normalized coefficient of variation averaged 3.7% across 12 control and target probes. This low imprecision allowed detection of 20% mosaicism with exceptional confidence (Pcomparision with reference populations. We have successfully incorporated aCGH confirmation using custom-designed MLPA into our normal workflow, and used it for confirmation of all abnormal or ambiguous results. PMID:21817904

  12. The Murchison Widefield Array: Design Overview

    OpenAIRE

    Lonsdale, Colin J.; Cappallo, Roger J.; Morales, Miguel F.; Briggs, Frank H.; Benkevitch, Leonid; Bowman, Judd D.; Bunton, John D.; Burns, Steven; Corey, Brian E.; deSouza, Ludi; Doeleman, Sheperd S.; Derome, Mark; Deshpande, Avinash; Gopalakrishna, M. R.; Greenhill, Lincoln J.

    2009-01-01

    The Murchison Widefield Array (MWA) is a dipole-based aperture array synthesis telescope designed to operate in the 80-300 MHz frequency range. It is capable of a wide range of science investigations, but is initially focused on three key science projects. These are detection and characterization of 3-dimensional brightness temperature fluctuations in the 21cm line of neutral hydrogen during the Epoch of Reionization (EoR) at redshifts from 6 to 10, solar imaging and remote sensing of the inn...

  13. Design of a Novel MEMS Gyroscope Array

    OpenAIRE

    Feng Sun; Wei Wang(College of William and Mary); Xiaoyong Lv

    2013-01-01

    This paper reports a novel four degree-of-freedom (DOF) MEMS vibratory gyroscope. A MEMS gyroscope array is then presented using the novel gyroscope unit. In the design of the proposed 4-DOF MEMS vibratory gyroscope, the elements of the drive-mode are set inside the whole gyroscope architecture, and the elements of sense-mode are set around the drive-mode, which thus makes it possible to combine several gyroscope units into a gyroscope array through sense-modes of all the units. The complete ...

  14. Design of a novel MEMS gyroscope array.

    Science.gov (United States)

    Wang, Wei; Lv, Xiaoyong; Sun, Feng

    2013-01-01

    This paper reports a novel four degree-of-freedom (DOF) MEMS vibratory gyroscope. A MEMS gyroscope array is then presented using the novel gyroscope unit. In the design of the proposed 4-DOF MEMS vibratory gyroscope, the elements of the drive-mode are set inside the whole gyroscope architecture, and the elements of sense-mode are set around the drive-mode, which thus makes it possible to combine several gyroscope units into a gyroscope array through sense-modes of all the units. The complete 2-DOF vibratory structure is utilized in both the drive-mode and sense-mode of the gyroscope unit, thereby providing the desired bandwidth and inherent robustness. The gyroscope array combines several gyroscope units by using the unique detection mass, which will increase the gain of sense-mode and improve the sensitivity of the system. The simulation results demonstrate that, compared to a single gyroscope unit, the gain of gyroscope array (n = 6) is increased by about 8 dB; a 3 dB bandwidth of 100 Hz in sense-mode and 190 Hz in drive-mode are also provided. The bandwidths of both modes are highly matched with each other, providing a bandwidth of 100 Hz for the entire system, thus illustrating that it could satisfy the requirements in practical applications.

  15. Flexible and accurate detection of genomic copy-number changes from aCGH.

    Directory of Open Access Journals (Sweden)

    Oscar M Rueda

    2007-06-01

    Full Text Available Genomic DNA copy-number alterations (CNAs are associated with complex diseases, including cancer: CNAs are indeed related to tumoral grade, metastasis, and patient survival. CNAs discovered from array-based comparative genomic hybridization (aCGH data have been instrumental in identifying disease-related genes and potential therapeutic targets. To be immediately useful in both clinical and basic research scenarios, aCGH data analysis requires accurate methods that do not impose unrealistic biological assumptions and that provide direct answers to the key question, "What is the probability that this gene/region has CNAs?" Current approaches fail, however, to meet these requirements. Here, we introduce reversible jump aCGH (RJaCGH, a new method for identifying CNAs from aCGH; we use a nonhomogeneous hidden Markov model fitted via reversible jump Markov chain Monte Carlo; and we incorporate model uncertainty through Bayesian model averaging. RJaCGH provides an estimate of the probability that a gene/region has CNAs while incorporating interprobe distance and the capability to analyze data on a chromosome or genome-wide basis. RJaCGH outperforms alternative methods, and the performance difference is even larger with noisy data and highly variable interprobe distance, both commonly found features in aCGH data. Furthermore, our probabilistic method allows us to identify minimal common regions of CNAs among samples and can be extended to incorporate expression data. In summary, we provide a rigorous statistical framework for locating genes and chromosomal regions with CNAs with potential applications to cancer and other complex human diseases.

  16. The Murchison Widefield Array: Design Overview

    CERN Document Server

    Lonsdale, Colin J; Morales, Miguel F; Briggs, Frank H; Benkevitch, Leonid; Bowman, Judd D; Bunton, John D; Burns, Steven; Corey, Brian E; deSouza, Ludi; Doeleman, Sheperd S; Derome, Mark; Deshpande, Avinash; Gopalakrishna, M R; Greenhill, Lincoln J; Herne, David; Hewitt, Jacqueline N; Kamini, P A; Kasper, Justin C; Kincaid, Barton B; Kocz, Jonathan; Kowald, Errol; Kratzenberg, Eric; Kumar, Deepak; Lynch, Mervyn J; Madhavi, S; Matejek, Michael; Mitchell, Daniel; Morgan, Edward; Oberoi, Divya; Ord, Steven; Pathikulangara, Joseph; Prabu, T; Rogers, Alan E E; Roshi, Anish; Salah, Joseph E; Sault, Robert J; Shankar, N Udaya; Srivani, K S; Stevens, Jamie; Tingay, Steven; Vaccarella, Annino; Waterson, Mark; Wayth, Randall B; Webster, Rachel L; Whitney, Alan R; Williams, Andrew; Williams, Christopher

    2009-01-01

    The Murchison Widefield Array (MWA) is a dipole-based aperture array synthesis telescope designed to operate in the 80-300 MHz frequency range. It is capable of a wide range of science investigations, but is initially focused on three key science projects. These are detection and characterization of 3-dimensional brightness temperature fluctuations in the 21cm line of neutral hydrogen during the Epoch of Reionization (EoR) at redshifts from 6 to 10, solar imaging and remote sensing of the inner heliosphere via propagation effects on signals from distant background sources,and high-sensitivity exploration of the variable radio sky. The array design features 8192 dual-polarization broad-band active dipoles, arranged into 512 tiles comprising 16 dipoles each. The tiles are quasi-randomly distributed over an aperture 1.5km in diameter, with a small number of outliers extending to 3km. All tile-tile baselines are correlated in custom FPGA-based hardware, yielding a Nyquist-sampled instantaneous monochromatic uv co...

  17. ADaCGH: A parallelized web-based application and R package for the analysis of aCGH data.

    Directory of Open Access Journals (Sweden)

    Ramón Díaz-Uriarte

    Full Text Available BACKGROUND: Copy number alterations (CNAs in genomic DNA have been associated with complex human diseases, including cancer. One of the most common techniques to detect CNAs is array-based comparative genomic hybridization (aCGH. The availability of aCGH platforms and the need for identification of CNAs has resulted in a wealth of methodological studies. METHODOLOGY/PRINCIPAL FINDINGS: ADaCGH is an R package and a web-based application for the analysis of aCGH data. It implements eight methods for detection of CNAs, gains and losses of genomic DNA, including all of the best performing ones from two recent reviews (CBS, GLAD, CGHseg, HMM. For improved speed, we use parallel computing (via MPI. Additional information (GO terms, PubMed citations, KEGG and Reactome pathways is available for individual genes, and for sets of genes with altered copy numbers. CONCLUSIONS/SIGNIFICANCE: ADACGH represents a qualitative increase in the standards of these types of applications: a all of the best performing algorithms are included, not just one or two; b we do not limit ourselves to providing a thin layer of CGI on top of existing BioConductor packages, but instead carefully use parallelization, examining different schemes, and are able to achieve significant decreases in user waiting time (factors up to 45x; c we have added functionality not currently available in some methods, to adapt to recent recommendations (e.g., merging of segmentation results in wavelet-based and CGHseg algorithms; d we incorporate redundancy, fault-tolerance and checkpointing, which are unique among web-based, parallelized applications; e all of the code is available under open source licenses, allowing to build upon, copy, and adapt our code for other software projects.

  18. Design & fabrication of cantilever array biosensors

    Directory of Open Access Journals (Sweden)

    Anja Boisen

    2009-09-01

    Full Text Available Surface immobilization of functional receptors on microfabricated cantilever arrays offers a new paradigm for the development of biosensors based on nanomechanics. Microcantilever-based systems are capable of real-time, multiplexed detection of unlabeled disease markers in extremely small volumes of samples. Currently available fabrication technology will allow the integration of electronic readout and sample introduction into a single unit, decreasing the device size, detection time, and cost. Biosensing technologies based on microfabricated cantilever arrays involving multiple cantilevers, electronic processing, and even local telemetry on a single chip have the potential of satisfying the need for highly sensitive and selective multiple-target detection in very small samples. Here we will review the design and fabrication process of cantilever-based biosensors.

  19. Conformal array design on arbitrary polygon surface with transformation optics

    Science.gov (United States)

    Deng, Li; Wu, Yongle; Hong, Weijun; Zhu, Jianfeng; Peng, Biao; Li, Shufang

    2016-06-01

    A transformation-optics based method to design a conformal antenna array on an arbitrary polygon surface is proposed and demonstrated in this paper. This conformal antenna array can be adjusted to behave equivalently as a uniformly spaced linear array by applying an appropriate transformation medium. An typical example of general arbitrary polygon conformal arrays, not limited to circular array, is presented, verifying the proposed approach. In summary, the novel arbitrary polygon surface conformal array can be utilized in array synthesis and beam-forming, maintaining all benefits of linear array.

  20. Conformal array design on arbitrary polygon surface with transformation optics

    Directory of Open Access Journals (Sweden)

    Li Deng

    2016-06-01

    Full Text Available A transformation-optics based method to design a conformal antenna array on an arbitrary polygon surface is proposed and demonstrated in this paper. This conformal antenna array can be adjusted to behave equivalently as a uniformly spaced linear array by applying an appropriate transformation medium. An typical example of general arbitrary polygon conformal arrays, not limited to circular array, is presented, verifying the proposed approach. In summary, the novel arbitrary polygon surface conformal array can be utilized in array synthesis and beam-forming, maintaining all benefits of linear array.

  1. A multiplex PCR predictor for aCGH success of FFPE samples.

    NARCIS (Netherlands)

    Beers, E.H. van; Joosse, S.A.; Ligtenberg, M.J.L.; Fles, R.; Hogervorst, F.B.L.; Verhoef, S.; Nederlof, P.M.

    2006-01-01

    Formalin-fixed, paraffin-embedded (FFPE) tissue archives are the largest and longest time-spanning collections of patient material in pathology archives. Methods to disclose information with molecular techniques, such as array comparative genomic hybridisation (aCGH) have rapidly developed but are s

  2. Design of a trichromatic cone array.

    Directory of Open Access Journals (Sweden)

    Patrick Garrigan

    2010-02-01

    Full Text Available Cones with peak sensitivity to light at long (L, medium (M and short (S wavelengths are unequal in number on the human retina: S cones are rare (<10% while increasing in fraction from center to periphery, and the L/M cone proportions are highly variable between individuals. What optical properties of the eye, and statistical properties of natural scenes, might drive this organization? We found that the spatial-chromatic structure of natural scenes was largely symmetric between the L, M and S sensitivity bands. Given this symmetry, short wavelength attenuation by ocular media gave L/M cones a modest signal-to-noise advantage, which was amplified, especially in the denser central retina, by long-wavelength accommodation of the lens. Meanwhile, total information represented by the cone mosaic remained relatively insensitive to L/M proportions. Thus, the observed cone array design along with a long-wavelength accommodated lens provides a selective advantage: it is maximally informative.

  3. Rectangular Thinned Array Design by McFarland Difference Sets

    OpenAIRE

    Oliveri, Giacomo; Caramanica, Federico; Rocca, Paolo; Massa, Andrea

    2011-01-01

    The problem of reducing the number of elements of large arrays is of great importance in satellite, remote sensing, radar and biomedical imaging applications in which the cost, weight, power consumption, mutual coupling effects, HW and SW complexity have to be as low as possible. Thinned arrays, however, are known to exhibit high peak sidelobe levels (PSL) if not suitably designed [1]. As a consequence, design techniques able to control and reduce the PSL of non‐regular arrays have been subje...

  4. 10-m platform array telescope design for the AMIBA project

    Science.gov (United States)

    Martin, Robert N.; Kingsley, Jeffrey S.

    2000-07-01

    We present a unique hexapod platform array for the new Taiwanese AMIBA project. AMIBA is a 90 GHz radio interferometric array consisting of 19 elements mounted on a roughly 10 m diameter platform. A hexapod mount is used to steer this platform. The resulting design is lightweight in comparison to a more conventional mount. The design goals of pointing stability, platform accuracy and reduced cost can be met with this design. A metrology system for pointing is proposed for inclusion in the design.

  5. Ladder Arrangement Method for Stealth Design of Vivaldi Antenna Array

    Directory of Open Access Journals (Sweden)

    XiaoXiang He

    2013-01-01

    Full Text Available A novel stealth design method for X-band Vivaldi antenna arrays is proposed in this paper by ladder arrangement along radiation direction. Two-element array, eight-element array, and 3 × 7-element array are investigated in this paper. S parameters, RCSs, and radiation patterns are studied, respectively. According to the ladder arrangement of Vivaldi antennas presented, 16.3 dBsm maximal RCS reduction is achieved with satisfied radiation performance. As simulated and measured, results demonstrate that the effectiveness of the presented low RCS design method is validated.

  6. Optimized low-cost-array field designs for photovoltaic systems

    Energy Technology Data Exchange (ETDEWEB)

    Post, H.N.; Carmichael, D.C.; Castle, J.A.

    1982-01-01

    As manager of the US Department of Energy Photovoltaic Systems Definition Project, Sandia National Laboratories is engaged in a comprehensive program to define and develop array field subsystems which can achieve the lowest possible lifecycle costs. The major activity of this program is described, namely, the design and development of optimized, modular array fields for photovoltaic (PV) systems. As part of this activity, design criteria and performance requirements for specific array subsystems including support structures, foundations, intermodule connections, field wiring, lightning protection, system grounding, site preparation, and monitoring and control have been defined and evaluated. Similarly, fully integrated flat-panel array field designs, optimized for lowest lifecycle costs, have been developed for system sizes ranging from 20 to 500 kW/sub p/. Key features, subsystem requirements, and projected costs for these array field designs are presented and discussed.

  7. A multi-sample based method for identifying common CNVs in normal human genomic structure using high-resolution aCGH data.

    Directory of Open Access Journals (Sweden)

    Chihyun Park

    Full Text Available BACKGROUND: It is difficult to identify copy number variations (CNV in normal human genomic data due to noise and non-linear relationships between different genomic regions and signal intensity. A high-resolution array comparative genomic hybridization (aCGH containing 42 million probes, which is very large compared to previous arrays, was recently published. Most existing CNV detection algorithms do not work well because of noise associated with the large amount of input data and because most of the current methods were not designed to analyze normal human samples. Normal human genome analysis often requires a joint approach across multiple samples. However, the majority of existing methods can only identify CNVs from a single sample. METHODOLOGY AND PRINCIPAL FINDINGS: We developed a multi-sample-based genomic variations detector (MGVD that uses segmentation to identify common breakpoints across multiple samples and a k-means-based clustering strategy. Unlike previous methods, MGVD simultaneously considers multiple samples with different genomic intensities and identifies CNVs and CNV zones (CNVZs; CNVZ is a more precise measure of the location of a genomic variant than the CNV region (CNVR. CONCLUSIONS AND SIGNIFICANCE: We designed a specialized algorithm to detect common CNVs from extremely high-resolution multi-sample aCGH data. MGVD showed high sensitivity and a low false discovery rate for a simulated data set, and outperformed most current methods when real, high-resolution HapMap datasets were analyzed. MGVD also had the fastest runtime compared to the other algorithms evaluated when actual, high-resolution aCGH data were analyzed. The CNVZs identified by MGVD can be used in association studies for revealing relationships between phenotypes and genomic aberrations. Our algorithm was developed with standard C++ and is available in Linux and MS Windows format in the STL library. It is freely available at: http://embio.yonsei.ac.kr/~Park/mgvd.php.

  8. Designing microcapsule arrays that propagate chemical signals

    Science.gov (United States)

    Bhattacharya, Amitabh; Balazs, Anna C.

    2010-08-01

    Using analysis and simulation, we show how ordered arrays of microcapsules in solution can be harnessed to propagate chemical signals in directed and controllable ways, allowing the signals to be transmitted over macroscopic distances. The system encompasses two types of capsules that are localized on an adhesive surface. The “signaling” capsules release inducer molecules, which trigger “targets” to release nanoparticles. The released nanoparticles can bind to the underlying surface and thus, create adhesion gradients, which then propel the signaling capsules to shuttle between neighboring targets. This arrangement acts like a relay, so that triggering target capsules at a particular location in the array also triggers target capsules in adjacent locations. For an array containing two target columns, our simulations and analysis show that steady input signal leads to a sustained periodic output. For an array containing multiple target columns, we show that by introducing a prescribed ratio of nanoparticle release rates between successive target columns, a chemical signal can be propagated along the array without dissipation. We also demonstrate that similar signal transmission cannot be performed via diffusion alone.

  9. Probe design for expression arrays using OligoWiz

    DEFF Research Database (Denmark)

    Wernersson, Rasmus

    2014-01-01

    Since all measurements from a DNA microarray is dependant on the probes used, a good choice of probes is of vital importa nce when designing custom micro-arrays. This chapter describes how to de sign expression arrays using the “ OligoWiz ” software suite. The general desired features of good...... probes and the issues which probe design must address are introduced and a conceptual (rather than mathematical) description of how OligoWiz scores the quality of th e potential probes is presented. This is followed by a detailed step-by-step guide to designing expression arrays with OligoWiz....

  10. Utilizing Amniotic Fluid Cell Karyotype Analysis and Array-CGH to Confirm NIPT Results and Discuss the Clinical Significance%羊水细胞染色体核型分析和微列阵比较基因组杂交技术验证无创产前检测的临床意义

    Institute of Scientific and Technical Information of China (English)

    周静; 蒋涛; 李璃; 刘安; 季修庆; 林颖; 王艳; 胡平; 许争峰

    2015-01-01

    目的:探讨羊水细胞染色体核型分析技术和微列阵比较基因组杂交技术(array-CGH)验证无创产前检测(NIPT)的临床意义.方法:对NIPT提示信号异常的95例孕妇行羊膜腔穿刺术,抽取羊水进行培养后行染色体核型分析验证其符合率;同时对提示除外21-三体、18-三体、13-三体的常染色体异常(即其他常染色体异常)的患者行array-CGH分析,验证其符合率.结果:NIPT提示21-三体高风险的染色体核型分析符合率86.96%(40/46);18-三体的染色体核型分析符合率76.92%(10/13);13--体染色体核型分析符合率0(0/2).性染色体核型分析的符合率50.00%(9/18),其中1例性染色体异常的染色体核型分析为46,XX,del(Xq23-25),行array-CGH验证,提示为X染色体该区带11.9M的片段缺失.其他常染色体异常的染色体核型分析符合率12.50% (2/16),其array-CGH验证的符合率25.00(4/16).结论:NIPT的结果需要验证,经典的羊水细胞染色体核型分析技术可以验证胎儿染色体数目和结构异常,array-CGH可以验证微缺失或者微重复,分辨率更高.

  11. Electronics design of a PET detector module with APD array

    CERN Document Server

    Wang Yong

    2002-01-01

    The author summarizes the advantages of APD-array for using in PET scanner. The front-end electronics for an experimental APD detector module was built and tested. According to the characteristics of APD-array and the demands of the signal readout in PET scanner, the full electronics system of an APD detector module was designed and presented in detail

  12. Multispectral linear array (MLA) focal plane mechanical and thermal design

    Science.gov (United States)

    Mitchell, A. S.; Kaminski, E. F.

    1982-01-01

    The mechanical and thermal design of an integrated focal plane subsystem of a Multispectral Linear Array (MLA) instrument is discussed in terms of focal-plane alignment, thermoelastic performance, and thermal requirements. The modular construction and thermal control of the focal plane array are discussed.

  13. Design of an active array filtenna for radar applications

    NARCIS (Netherlands)

    Cifola, L.; Gerini, G.; Berg, S. van den; Water, F. van de

    2014-01-01

    A novel design of an S-band active array antenna with enhanced frequency selectivity properties, for radar applications, is presented. The array unit cell consists of a stacked-patch radiator, characterized by an operational bandwidth of [2.8 - 3.4] GHz. A microstrip two-pole band pass filter is con

  14. Design of a new electrode array for cochlear implants

    International Nuclear Information System (INIS)

    Full text: This study aims to design a new electrode array which can be precisely located beneath the basilar membrane within the cochlear scala tympani. This placement of the electrode array is beneficial for increasing the effectiveness of the electrical stimulation of the audi tory nerves and maximising the growth factors delivered into the cochlea for regenerating the progressively lost auditory neurons, thereby significantly improving performance of the cochlear implant systems. Methods The design process involved two steps. First, the biocom patible nitinol-based shape memory alloy, of which mechanical deformation can be controlled using electrical cUTents/fields act vated by body temperature, was selected. Second, five different designs of the electrode array with embedded nitinol actuators were studied (Table I). The finite element method was employed to predict final positions of these electrode arrays. Results The electrode array with three 6 mm actuators at 2-8, 8-J4 and 14-20 mm from the tip (Fig. I) was found to be located most closely to the basilar membrane, compared with those in the other four cases. Conclusions A new nitinol cochlear implant electrode array with three embedded nitinol actuators has been designed. This electrode array is expected to be located beneath the basilar membrane for maximising the delivery of growth factors. Future research will involve the manufacturing of a prototype of this electrode array for use in insertion experiments and neurotrophin release tests.

  15. Design & fabrication of cantilever array biosensors

    DEFF Research Database (Denmark)

    Boisen, Anja; Thundat, T

    2009-01-01

    Surface immobilization of functional receptors on microfabricated cantilever arrays offers a new paradigm for the development of biosensors based on nanomechanics. Microcantilever-based systems are capable of real-time, multiplexed detection of unlabeled disease markers in extremely small volumes...

  16. Optical Design of Segmented Hexagon Array Solar Mirror

    Science.gov (United States)

    Huegele, Vince

    2000-01-01

    A segmented array of mirrors was designed for a solar concentrator test stand at MSFC for firing solar thermal propulsion engines. The 144 mirrors each have a spherical surface to approximate a parabolic concentrator when combined into the entire 18-foot diameter array. The mirror segments are aluminum hexagons that had the surface diamond turned and quartz coated. The array focuses sunlight reflected from a heliostat to a 4 inch diameter spot containing 10 kw of power at the 15-foot focal point. The derivation of the surface figure for the respective mirror elements is shown. The alignment process of the array is discussed and test results of the system's performance is given.

  17. Array antennas design in dependence of element-phasing

    Science.gov (United States)

    Zichner, R.; Chandra, M.

    2009-05-01

    Array antennas are used in science as well as for commercial and military purposes. The used element antennas act in accordance to their desired uses, for example radars or stationer GPS satellites. Typical components are for example slotted waveguides, patches, yagi-antennas and helix-antennas. All these elements do stand out with their own characteristics based on their special applications. If these elements are formed into an array configuration, the effectiveness can be improved immensely. There is a relation between the array functions and the physical array properties like the element alignment (linear, planar, circular), distances between the elements and so on. Among the physical properties there are other attributes like phase or amplitude coefficients, which are of great significance. The aim of this study was to provide an insight into the problem of array design, as far as the antenna element phase is concerned. Along with this, array radiation characteristics effects are presented. With the help of the extracted cognitions beam forming behaviour can be shown and the array phase behaviour can be analysed. One of the main applications is to simulate the array characteristics, like the radiation characteristic or the gain, for displacements of the array feeding point. A software solution that simulates the phase shift of a given array pattern is sought to adjust the feeding point.

  18. Array antennas design in dependence of element-phasing

    Directory of Open Access Journals (Sweden)

    R. Zichner

    2009-05-01

    Full Text Available Array antennas are used in science as well as for commercial and military purposes. The used element antennas act in accordance to their desired uses, for example radars or stationer GPS satellites. Typical components are for example slotted waveguides, patches, yagi-antennas and helix-antennas. All these elements do stand out with their own characteristics based on their special applications. If these elements are formed into an array configuration, the effectiveness can be improved immensely. There is a relation between the array functions and the physical array properties like the element alignment (linear, planar, circular, distances between the elements and so on. Among the physical properties there are other attributes like phase or amplitude coefficients, which are of great significance. The aim of this study was to provide an insight into the problem of array design, as far as the antenna element phase is concerned. Along with this, array radiation characteristics effects are presented. With the help of the extracted cognitions beam forming behaviour can be shown and the array phase behaviour can be analysed. One of the main applications is to simulate the array characteristics, like the radiation characteristic or the gain, for displacements of the array feeding point. A software solution that simulates the phase shift of a given array pattern is sought to adjust the feeding point.

  19. Design and Analysis Tools for Deployable Solar Array Systems Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Large, lightweight, deployable solar array structures have been identified as a key enabling technology for NASA with analysis and design of these structures being...

  20. Discrete Dipole Approximation Aided Design Method for Nanostructure Arrays

    Institute of Scientific and Technical Information of China (English)

    ZHU Shao-Li; LUO Xian-Gang; DU Chun-Lei

    2007-01-01

    A discrete dipole approximation (DDA) aided design method is proposed to determine the parameters of nanostructure arrays. The relationship between the thickness, period and extinction efficiency of nanostructure arrays for the given shape can be calculated using the DDA. Based on the calculated curves, the main parameters of the nanostructure arrays such as thickness and period can be determined. Using this aided method, a rhombic sliver nanostructure array is designed with the determinant parameters of thickness (40 nm) and period (440 nm).We further fabricate the rhombic sliver nanostructure arrays and testify the character of the extinction spectra.The obtained extinction spectra is within the visible range and the full width at half maximum is 99nm, as is expected.

  1. APRON: A Cellular Processor Array Simulation and Hardware Design Tool

    Directory of Open Access Journals (Sweden)

    David R. W. Barr

    2009-01-01

    Full Text Available We present a software environment for the efficient simulation of cellular processor arrays (CPAs. This software (APRON is used to explore algorithms that are designed for massively parallel fine-grained processor arrays, topographic multilayer neural networks, vision chips with SIMD processor arrays, and related architectures. The software uses a highly optimised core combined with a flexible compiler to provide the user with tools for the design of new processor array hardware architectures and the emulation of existing devices. We present performance benchmarks for the software processor array implemented on standard commodity microprocessors. APRON can be configured to use additional processing hardware if necessary and can be used as a complete graphical user interface and development environment for new or existing CPA systems, allowing more users to develop algorithms for CPA systems.

  2. Nanowire array photovoltaics: Radial disorder versus design for optimal efficiency

    CERN Document Server

    Sturmberg, Björn C P; Botten, Lindsay C; Asatryan, Ara A; Poulton, Christopher G; McPhedran, Ross C; de Sterke, C Martijn

    2014-01-01

    Solar cell designs based on disordered nanostructures tend to have higher efficiencies than structures with uniform absorbers, though the reason is poorly understood. To resolve this, we use a semi-analytic approach to determine the physical mechanism leading to enhanced efficiency in arrays containing nanowires with a variety of radii. We use our findings to systematically design arrays that outperform randomly composed structures. An ultimate efficiency of 23.75% is achieved with an array containing 30% silicon, an increase of almost 10% over a homogeneous film of equal thickness.

  3. Introduction to embedded system design using field programmable gate arrays

    CERN Document Server

    Dubey, Rahul

    2009-01-01

    Offers information on the use of field programmable gate arrays (FPGAs) in the design of embedded systems. This text considers a hypothetical robot controller as an embedded application and weaves around it related concepts of FPGA-based digital design. It is suitable for both students and designers who have worked with microprocessors.

  4. Optimum array design to maximize Fisher information for bearing estimation.

    Science.gov (United States)

    Tuladhar, Saurav R; Buck, John R

    2011-11-01

    Source bearing estimation is a common application of linear sensor arrays. The Cramer-Rao bound (CRB) sets a lower bound on the achievable mean square error (MSE) of any unbiased bearing estimate. In the spatially white noise case, the CRB is minimized by placing half of the sensors at each end of the array. However, many realistic ocean environments have a mixture of both white noise and spatially correlated noise. In shallow water environments, the correlated ambient noise can be modeled as cylindrically isotropic. This research designs a fixed aperture linear array to maximize the bearing Fisher information (FI) under these noise conditions. The FI is the inverse of the CRB, so maximizing the FI minimizes the CRB. The elements of the optimum array are located closer to the array ends than uniform spacing, but are not as extreme as in the white noise case. The optimum array results from a trade off between maximizing the array bearing sensitivity and minimizing output noise power variation over the bearing. Depending on the source bearing, the resulting improvement in MSE performance of the optimized array over a uniform array is equivalent to a gain of 2-5 dB in input signal-to-noise ratio. PMID:22087908

  5. Design of magnet arrays for permanent magnetic linear motor

    Institute of Scientific and Technical Information of China (English)

    Junhong MAO; Junhang LUO; Qiang JIANG; Youbai XIE

    2008-01-01

    An iron-less single side permanent magnetic linear motor structure is presented,and two-dimensional analytical formulae for its magnetic field are deduced to design the types of magnetic arrays,the thickness of permanent magnet,and the duty ratio of magnet arrays,etc.With certain design parameters,conventional arrays are used instead of a piecewise Halbach arrays,and the machining and assembling of the motor are greatly simplified.Calculation results coincide with that of ANSYS.The analytical formulae can be used in designing cored linear motors by modifying boundary conditions.A linear motor driven bench with a single degree of freedom is realized,where the travel reaches 27 mm,the mass of the moving parts is 1.4 kg,and the maximum acceleration iS 11.5 m/s2.

  6. Phased-array radar design application of radar fundamentals

    CERN Document Server

    Jeffrey, Thomas

    2009-01-01

    Phased-Array Radar Design is a text-reference designed for electrical engineering graduate students in colleges and universities as well as for corporate in-house training programs for radar design engineers, especially systems engineers and analysts who would like to gain hands-on, practical knowledge and skills in radar design fundamentals, advanced radar concepts, trade-offs for radar design and radar performance analysis.

  7. The design and test of MEMS piezoresistive ultrasonic sensor arrays

    Institute of Scientific and Technical Information of China (English)

    Lian Deqin; He Changde; Zhang Hui; Yu Jiaqi; Yuan Kejing; Xue Chenyang

    2013-01-01

    The design,fabrication and packaging of a type of MEMS piezoresistive ultrasonic transducer array are introduced.The consistency of the resonance frequency and the sensitivity of the array are tested.Moreover,we detect the directivity and the multi-target identification ability of the array.The results of the consistency of the resonance frequency and the sensitivity show that there is a gap between the practical and theoretical results.This paper analyzes this problem in detail and points out the direction of improvement.As for the directivity,the actual result is consistent with the theoretical one.The results of multiple target distinguishing tests demonstrate that the smallest resolution angle of the array is 5.72° when the distance between the sensor array and measured objects is 2m.

  8. Detection of genomic copy number changes in patients with idiopathic mental retardation by high-resolution X-array-CGH: important role for increased gene dosage of XLMR genes.

    Science.gov (United States)

    Froyen, Guy; Van Esch, Hilde; Bauters, Marijke; Hollanders, Karen; Frints, Suzanna G M; Vermeesch, Joris R; Devriendt, Koen; Fryns, Jean-Pierre; Marynen, Peter

    2007-10-01

    A tiling X-chromosome-specific genomic array with a theoretical resolution of 80 kb was developed to screen patients with idiopathic mental retardation (MR) for submicroscopic copy number differences. Four patients with aberrations previously detected at lower resolution were first analyzed. This facilitated delineation of the location and extent of the aberration at high resolution and subsequently, more precise genotype-phenotype analyses. A cohort of 108 patients was screened, 57 of which were suspected of X-linked mental retardation (XLMR), 26 were probands of brother pairs, and 25 were sporadic cases. A total of 15 copy number changes in 14 patients (13%) were detected, which included two deletions and 13 duplications ranging from 0.1 to 2.7 Mb. The aberrations are associated with the phenotype in five patients (4.6%), based on the following criteria: de novo aberration; involvement of a known or candidate X-linked nonsyndromic(syndromic) MR (MRX(S)) gene; segregation with the disease in the family; absence in control individuals; and skewed X-inactivation in carrier females. These include deletions that contain the MRX(S) genes CDKL5, OPHN1, and CASK, and duplications harboring CDKL5, NXF5, MECP2, and GDI1. In addition, seven imbalances were apparent novel polymorphic regions because they do not fulfill the proposed criteria. Taken together, our data strongly suggest that not only deletions but also duplications on the X chromosome contribute to the phenotype more often than expected, supporting the increased gene dosage mechanism for deregulation of normal cognitive development.

  9. Design of the L-SAT solar array

    Energy Technology Data Exchange (ETDEWEB)

    Gerlach, L.; Marks, G.W.; Quittner, E.; Renshall, J.

    1982-06-01

    The concept, requirements, mission phases, design, analyses, and testing of a large, modular, deployable, flexible/foldable blanket, Sun tracking solar array are described. The same design is capable of providing 1.9 kW to 6.5 kW end-of-10 yr geosynchronous equinox power per spacecraft by simple modular changes, with further growth to 7.8 kW by means of structral modifications. The array undergoes partial deployment after insertion into the transfer orbit to develop 3 kW minimum and is fully deployed after apogee engine firing.

  10. Compressive Sensing Based Design of Sparse Tripole Arrays.

    Science.gov (United States)

    Hawes, Matthew; Liu, Wei; Mihaylova, Lyudmila

    2015-12-10

    This paper considers the problem of designing sparse linear tripole arrays. In such arrays at each antenna location there are three orthogonal dipoles, allowing full measurement of both the horizontal and vertical components of the received waveform. We formulate this problem from the viewpoint of Compressive Sensing (CS). However, unlike for isotropic array elements (single antenna), we now have three complex valued weight coefficients associated with each potential location (due to the three dipoles), which have to be simultaneously minimised. If this is not done, we may only set the weight coefficients of individual dipoles to be zero valued, rather than complete tripoles, meaning some dipoles may remain at each location. Therefore, the contributions of this paper are to formulate the design of sparse tripole arrays as an optimisation problem, and then we obtain a solution based on the minimisation of a modified l1 norm or a series of iteratively solved reweighted minimisations, which ensure a truly sparse solution. Design examples are provided to verify the effectiveness of the proposed methods and show that a good approximation of a reference pattern can be achieved using fewer tripoles than a Uniform Linear Array (ULA) of equivalent length.

  11. Compressive Sensing Based Design of Sparse Tripole Arrays

    Directory of Open Access Journals (Sweden)

    Matthew Hawes

    2015-12-01

    Full Text Available This paper considers the problem of designing sparse linear tripole arrays. In such arrays at each antenna location there are three orthogonal dipoles, allowing full measurement of both the horizontal and vertical components of the received waveform. We formulate this problem from the viewpoint of Compressive Sensing (CS. However, unlike for isotropic array elements (single antenna, we now have three complex valued weight coefficients associated with each potential location (due to the three dipoles, which have to be simultaneously minimised. If this is not done, we may only set the weight coefficients of individual dipoles to be zero valued, rather than complete tripoles, meaning some dipoles may remain at each location. Therefore, the contributions of this paper are to formulate the design of sparse tripole arrays as an optimisation problem, and then we obtain a solution based on the minimisation of a modified l 1 norm or a series of iteratively solved reweighted minimisations, which ensure a truly sparse solution. Design examples are provided to verify the effectiveness of the proposed methods and show that a good approximation of a reference pattern can be achieved using fewer tripoles than a Uniform Linear Array (ULA of equivalent length.

  12. Design of nested Halbach cylinder arrays for magnetic refrigeration applications

    Energy Technology Data Exchange (ETDEWEB)

    Trevizoli, Paulo V., E-mail: trevizoli@polo.ufsc.br; Lozano, Jaime A.; Peixer, Guilherme F.; Barbosa Jr, Jader R.

    2015-12-01

    We present an experimentally validated analytical procedure to design nested Halbach cylinder arrays for magnetic cooling applications. The procedure aims at maximizing the magnetic flux density variation in the core of the array for a given set of design parameters, namely the inner diameter of the internal magnet, the air gap between the magnet cylinders, the number of segments of each magnet and the remanent flux density of the Nd{sub 2}Fe{sub 14}B magnet grade. The design procedure was assisted and verified by 3-D numerical modeling using a commercial software package. An important aspect of the optimal design is to maintain an uniform axial distribution of the magnetic flux density in the region of the inner gap occupied by the active magnetocaloric regenerator. An optimal nested Halbach cylinder array was manufactured and experimentally evaluated for the magnetic flux density in the inner gap. The analytically calculated magnetic flux density variation agreed to within 5.6% with the experimental value for the center point of the magnet gap. - Highlights: • An analytical procedure to design nested Halbach cylinder arrays is proposed. • An optimal magnet configuration was built based on the analytical procedure. • The procedure was validated with 3D COMSOL simulations and experimental data.

  13. Active array design for FAME: Freeform Active Mirror Experiment

    Science.gov (United States)

    Jaskó, Attila; Aitink-Kroes, Gabby; Agócs, Tibor; Venema, Lars; Hugot, Emmanuel; Schnetler, Hermine; Bányai, Evelin

    2014-07-01

    In this paper a status report is given on the development of the FAME (Freeform Active Mirror Experiment) active array. Further information regarding this project can be found in the paper by Venema et al. (this conference). Freeform optics provide the opportunity to drastically reduce the complexity of the future optical instruments. In order to produce these non-axisymmetric freeform optics with up to 1 mm deviation from the best fit sphere, it is necessary to come up with new design and manufacturing methods. The way we would like to create novel freeform optics is by fine tuning a preformed high surface-quality thin mirror using an array which is actively controlled by actuators. In the following we introduce the tools deployed to create and assess the individual designs. The result is an active array having optimal number and lay-out of actuators.

  14. límites organizacionales del CGH

    Directory of Open Access Journals (Sweden)

    Carlos Chávez Becker

    2005-01-01

    Full Text Available El presente trabajo se centra en el análisis de los mecanismos que el Consejo General de Huelga empleó para tomar decisiones, su esquema de representación y su forma de operación política con el fin de establecer algunas relaciones significativas entre tales elementos analíticos y los resultados obtenidos por la organización al término del conflicto en febrero de 2000. La principal intención es evaluar al CGH como la organización de los estudiantes en términos de su actuar político a la luz de los costos y beneficios que representó su actividad.

  15. The design of series-parallel connected thermionic converter arrays

    International Nuclear Information System (INIS)

    Thermionic converters are connected together in a series-parallel array in order to provide a useful output voltage. A model is presented to predict the effects of a nonuniform distribution of input power on the performance of such an array. Three cases are considered: where (1) the heat flux is uniform (2) the heat flux nonuniformity is in the direction of series connections in the array (3) the heat flux nonuniformity is in the direction of parallel connections. The effect of optimizing cesium pressure for the individual converters is also studied. Results show that the heat flux variation in the series direction can cause a 20-40% drop in array output power as compared to a uniform heat input, as well as an unacceptable variation in emitter temperatures. Heat flux variation along the parallel connections without optimizing cesium pressure causes a 38% loss of array output power and very high emitter temperatures in some converters. Optimization of the cesium pressure in this case, however, reduces the output power loss to 12% and gives emitter temperatures which are all within an acceptable range. Thus, given a proper design, series-parallel arrays can be used to accommodate wide ranges of heat input while still delivering good performance

  16. Conceptual design of a hybrid Ge:Ga detector array

    Science.gov (United States)

    Parry, C. M.

    1984-01-01

    For potential applications in space infrared astronomy missions such as the Space Infrared Telescope Facility and the Large Deployable Reflector, integrated arrays of long-wavelength detectors are desired. The results of a feasibility study which developed a design for applying integrated array techniques to a long-wavelength (gallium-doped germanium) material to achieve spectral coverage between 30 and 200 microns are presented. An approach which builds up a two-dimensional array by stacking linear detector modules is presented. The spectral response of the Ge:Ga detectors is extended to 200 microns by application of uniaxial stress to the stack of modules. The detectors are assembled with 1 mm spacing between the elements. Multiplexed readout of each module is accomplished with integration sampling of a metal-oxide-semiconductor (MOS) switch chip. Aspects of the overall design, including the anticipated level of particle effects on the array in the space environment, a transparent electrode design for 200 microns response, estimates of optical crosstalk, and mechanical stress design calculations are included.

  17. Approximate Dynamic Programming for Fast Denoising of aCGH Data

    CERN Document Server

    Miller, Gary L; Schwartz, Russell; Tsourakakis, Charalampos E

    2010-01-01

    DNA sequence copy number is the number of copies of DNA at a region of a genome. Identifying genomic regions whose DNA copy number deviates from the normal is a crucial task in understanding cancer evolution. Array-based comparative genomic hybridization (aCGH) is a high-throughput technique for identifying DNA gain or loss. Due to the high level of noise in microarray data, however, interpretation of aCGH output is a difficult and error-prone task. In this paper, we adopt a recent formulation of the denoising aCGH data problem as a regularized least squares problem and propose an approximation algorithm within $\\epsilon$ additive error, where \\epsilon is an arbitrarily small positive constant. Specifically, we show that for n probes, we can approximate the optimal value of our function within additive \\epsilon with an algorithm that runs in $\\tilde{O}(n^{1.5} \\log{(\\frac{U}{\\epsilon}))}$ time, where U is the maximum value over the regularization term and the probes. The basis of our algorithm is the definiti...

  18. Design of Linear Systolic Arrays for Matrix Multiplication

    OpenAIRE

    MILOVANOVIC, E. I.; STOJCEV, M. K.; MILOVANOVIC, I. Z.; NIKOLIC, T. R.

    2014-01-01

    This paper presents architecture for matrix multiplication optimized to be integrated as an accelerator unit to a host computer. Two linear systolic arrays with unidirectional data flow (ULSA), used as hardware accelerators, where synthesized in this paper. The solution proposed here is designed to accelerate both the computation and communication by employing hardware address generator units (AGUs). The proposed design has been implemented on Xilinx Spartan-2E and Virtex4 FPG...

  19. Analysis and design of low profile multiband multifunctional antenna arrays

    Science.gov (United States)

    Hunsicker, Walker F.

    Light-weight phased array antennas for aerospace and mobile applications require utilizing the same antenna aperture to provide multiple functions with dissimilar radiation pattern specifications (e.g., multiband operation for communications and tracking). Multi-functional antennas provide advantages over aggregate antenna clusters by reducing space requirements, and can aid in the optimal placement of all required apertures to provide adequate isolation between channels. Furthermore, the combination of antenna apertures into a common geometry mitigates co-site installation issues by addressing interference within the integrated radiator design itself as opposed to the extensive analysis which is required to configure multiple radiators in close proximity. The combination of multiple radiators into a single aperture can only be achieved with the proper selection of antenna topology and accompanying feed network design. This research proposes a new technique for the design of multiband arrays in which a common aperture is used. Highlighted by this method is the integration of a tri-band array comprised of an X-band (12 GHz) microstrip patch array on a superstrate above printed dual-band (1 and 2 GHz) slot loop antenna arrays in an octave-spaced lattice. The selection of a ground backing reflector is considered for improved gain and system packaging, but restricts the utility of the design principally due to the lambda/4 depth of the ground plane. Therefore, a novel multiband high impedance surfaces (HIS) is proposed to load the slot apertures for reduced height. The novel techniques proposed here will enable the design of a low profile and conformal single aperture supporting multi-band and multi-functional operations.

  20. High resolution array-CGH analysis of single cells

    OpenAIRE

    Fiegler, Heike; Geigl, Jochen B.; Langer, Sabine; Rigler, Diane; Porter, Keith; Unger, Kristian; Carter, Nigel P; Speicher, Michael R.

    2006-01-01

    Heterogeneity in the genome copy number of tissues is of particular importance in solid tumor biology. Furthermore, many clinical applications such as pre-implantation and non-invasive prenatal diagnosis would benefit from the ability to characterize individual single cells. As the amount of DNA from single cells is so small, several PCR protocols have been developed in an attempt to achieve unbiased amplification. Many of these approaches are suitable for subsequent cytogenetic analyses usin...

  1. Small Area Array-Based LED Luminaire Design

    Energy Technology Data Exchange (ETDEWEB)

    Thomas Yuan

    2008-01-09

    This report contains a summary of technical achievements during a three-year project to demonstrate high efficiency LED luminaire designs based on small area array-based gallium nitride diodes. Novel GaN-based LED array designs are described, specifically addressing the thermal, optical, electrical and mechanical requirements for the incorporation of such arrays into viable solid-state LED luminaires. This work resulted in the demonstration of an integrated luminaire prototype of 1000 lumens cool white light output with reflector shaped beams and efficacy of 89.4 lm/W at CCT of 6000oK and CRI of 73; and performance of 903 lumens warm white light output with reflector shaped beams and efficacy of 63.0 lm/W at CCT of 2800oK and CRI of 82. In addition, up to 1275 lumens cool white light output at 114.2 lm/W and 1156 lumens warm white light output at 76.5 lm/W were achieved if the reflector was not used. The success to integrate small area array-based LED designs and address thermal, optical, electrical and mechanical requirements was clearly achieved in these luminaire prototypes with outstanding performance and high efficiency.

  2. Modern Design of Resonant Edge-Slot Array Antennas

    Science.gov (United States)

    Gosselin, R. B.

    2006-01-01

    Resonant edge-slot (slotted-waveguide) array antennas can now be designed very accurately following a modern computational approach like that followed for some other microwave components. This modern approach makes it possible to design superior antennas at lower cost than was previously possible. Heretofore, the physical and engineering knowledge of resonant edge-slot array antennas had remained immature since they were introduced during World War II. This is because despite their mechanical simplicity, high reliability, and potential for operation with high efficiency, the electromagnetic behavior of resonant edge-slot antennas is very complex. Because engineering design formulas and curves for such antennas are not available in the open literature, designers have been forced to implement iterative processes of fabricating and testing multiple prototypes to derive design databases, each unique for a specific combination of operating frequency and set of waveguide tube dimensions. The expensive, time-consuming nature of these processes has inhibited the use of resonant edge-slot antennas. The present modern approach reduces costs by making it unnecessary to build and test multiple prototypes. As an additional benefit, this approach affords a capability to design an array of slots having different dimensions to taper the antenna illumination to reduce the amplitudes of unwanted side lobes. The heart of the modern approach is the use of the latest commercially available microwave-design software, which implements finite-element models of electromagnetic fields in and around waveguides, antenna elements, and similar components. Instead of building and testing prototypes, one builds a database and constructs design curves from the results of computational simulations for sets of design parameters. The figure shows a resonant edge-slot antenna designed following this approach. Intended for use as part of a radiometer operating at a frequency of 10.7 GHz, this antenna

  3. Monte Carlo design studies for the Cherenkov Telescope Array

    CERN Document Server

    Bernlöhr, K; Becherini, Y; Bigas, O Blanch; Carmona, E; Colin, P; Decerprit, G; Di Pierro, F; Dubois, F; Farnier, C; Funk, S; Hermann, G; Hinton, J A; Humensky, T B; Khélifi, B; Kihm, T; Komin, N; Lenain, J -P; Maier, G; Mazin, D; Medina, M C; Moralejo, A; Nolan, S J; Ohm, S; Wilhelmi, E de Oña; Parsons, R D; Arribas, M Paz; Pedaletti, G; Pita, S; Prokoph, H; Rulten, C B; Schwanke, U; Shayduk, M; Stamatescu, V; Vallania, P; Vorobiov, S; Wischnewski, R; Yoshikoshi, T; Zech, A

    2012-01-01

    The Cherenkov Telescopes Array (CTA) is planned as the future instrument for very-high-energy (VHE) gamma-ray astronomy with a wide energy range of four orders of magnitude and an improvement in sensitivity compared to current instruments of about an order of magnitude. Monte Carlo simulations are a crucial tool in the design of CTA. The ultimate goal of these simulations is to find the most cost-effective solution for given physics goals and thus sensitivity goals or to find, for a given cost, the solution best suited for different types of targets with CTA. Apart from uncertain component cost estimates, the main problem in this procedure is the dependence on a huge number of configuration parameters, both in specifications of individual telescope types and in the array layout. This is addressed by simulation of a huge array intended as a superset of many different realistic array layouts, and also by simulation of array subsets for different telescope parameters. Different analysis methods -- in use with cu...

  4. Design of photovoltaic central power station concentrator array

    Energy Technology Data Exchange (ETDEWEB)

    1984-02-01

    A design for a photovoltaic central power station using tracking concentrators has been developed. The 100 MW plant is assumed to be located adjacent to the Saguaro Power Station of Arizona Public Service. The design assumes an advanced Martin Marietta two-axis tracking fresnel lens concentrator. The concentrators are arrayed in 5 MW subfields, each with its own power conditioning unit. The photovoltaic plant output is connected to the existing 115 kV switchyard. The site specific design allows detailed cost estimates for engineering, site preparation, and installation. Collector and power conditioning costs have been treated parametrically.

  5. Centro para la Salud Mundial (CGH) del NCI

    Science.gov (United States)

    El Centro para la Salud Mundial (CGH) del NCI coordina actividades de investigación y trabaja con socios nacionales e internacionales para comprender y enfrentar la carga que representa el cáncer a nivel mundial.

  6. Computational Methods for the Analysis of Array Comparative Genomic Hybridization

    Directory of Open Access Journals (Sweden)

    Raj Chari

    2006-01-01

    Full Text Available Array comparative genomic hybridization (array CGH is a technique for assaying the copy number status of cancer genomes. The widespread use of this technology has lead to a rapid accumulation of high throughput data, which in turn has prompted the development of computational strategies for the analysis of array CGH data. Here we explain the principles behind array image processing, data visualization and genomic profile analysis, review currently available software packages, and raise considerations for future software development.

  7. Design and Analysis of MEMS Linear Phased Array

    Directory of Open Access Journals (Sweden)

    Guoxiang Fan

    2016-01-01

    Full Text Available A structure of micro-electro-mechanical system (MEMS linear phased array based on “multi-cell” element is designed to increase radiation sound pressure of transducer working in bending vibration mode at high frequency. In order to more accurately predict the resonant frequency of an element, the theoretical analysis of the dynamic equation of a fixed rectangular composite plate and finite element method simulation are adopted. The effects of the parameters both in the lateral and elevation direction on the three-dimensional beam directivity characteristics are comprehensively analyzed. The key parameters in the analysis include the “cell” number of element, “cell” size, “inter-cell” spacing and the number of elements, element width. The simulation results show that optimizing the linear array parameters both in the lateral and elevation direction can greatly improve the three-dimensional beam focusing for MEMS linear phased array, which is obviously different from the traditional linear array.

  8. Solar cell array design handbook - The principles and technology of photovoltaic energy conversion

    Science.gov (United States)

    Rauschenbach, H. S.

    1980-01-01

    Photovoltaic solar cell array design and technology for ground-based and space applications are discussed from the user's point of view. Solar array systems are described, with attention given to array concepts, historical development, applications and performance, and the analysis of array characteristics, circuits, components, performance and reliability is examined. Aspects of solar cell array design considered include the design process, photovoltaic system and detailed array design, and the design of array thermal, radiation shielding and electromagnetic components. Attention is then given to the characteristics and design of the separate components of solar arrays, including the solar cells, optical elements and mechanical elements, and the fabrication, testing, environmental conditions and effects and material properties of arrays and their components are discussed.

  9. Guided Wave Annular Array Sensor Design for Improved Tomographic Imaging

    Science.gov (United States)

    Koduru, Jaya Prakash; Rose, Joseph L.

    2009-03-01

    Guided wave tomography for structural health monitoring is fast emerging as a reliable tool for the detection and monitoring of hotspots in a structure, for any defects arising from corrosion, crack growth etc. To date guided wave tomography has been successfully tested on aircraft wings, pipes, pipe elbows, and weld joints. Structures practically deployed are subjected to harsh environments like exposure to rain, changes in temperature and humidity. A reliable tomography system should take into account these environmental factors to avoid false alarms. The lack of mode control with piezoceramic disk sensors makes it very sensitive to traces of water leading to false alarms. In this study we explore the design of annular array sensors to provide mode control for improved structural tomography, in particular, addressing the false alarm potential of water loading. Clearly defined actuation lines in the phase velocity dispersion curve space are calculated. A dominant in-plane displacement point is found to provide a solution to the water loading problem. The improvement in the tomographic images with the annular array sensors in the presence of water traces is clearly illustrated with a series of experiments. An annular array design philosophy for other problems in NDE/SHM is also discussed.

  10. Neutron detector array at IUAC: Design features and instrumentation developments

    Indian Academy of Sciences (India)

    P Sugathan; A Jhingan; K S Golda; T Varughese; S Venkataramanan; N Saneesh; V V Satyanarayana; S K Suman; J Antony; Ruby Shanti; K Singh; S K Saini; A Gupta; A Kothari; P Barua; Rajesh Kumar; J Zacharias; R P Singh; B R Behera; S K Mandal; I M Govil; R K Bhowmik

    2014-11-01

    The characteristics and performance of the newly commissioned neutron detector array at IUAC are described. The array consists of 100 BC501 liquid scintillators mounted in a semispherical geometry and are kept at a distance of 175 cm from the reaction point. Each detector is a 5″ × 5″ cylindrical cell coupled to 5″ diameter photomultiplier tube (PMT). Signal processing is realized using custom-designed home-made integrated electronic modules which perform neutron–gamma discrimination using zero cross timing and time-of-flight (TOF) technique. Compact custom-built high voltage power supply developed using DC–DC converters are used to bias the detector. The neutrons are recorded in coincidence with fission fragments which are detected using multi-wire proportional counters mounted inside a 1m diameter SS target chamber. The detectors and electronics have been tested off-line using radioactive sources and the results are presented.

  11. Degenerate Oligonucleotide Primed-Polymerase Chain Reaction-Based Array Comparative Genomic Hybridization for Extensive Amplicon Profiling of Breast Cancers : A New Approach for the Molecular Analysis of Paraffin-Embedded Cancer Tissue

    OpenAIRE

    Daigo, Yataro; Chin, Suet-Feung; Gorringe, Kylie L.; Bobrow, Lynda G; Bruce A J Ponder; Pharoah, Paul D P; Caldas, Carlos

    2001-01-01

    We have developed a protocol for degenerate oligonucleotide-primed-polymerase chain reaction-based array comparative genomic hybridization (array CGH) that, when combined with a laser microdissection technique, allows the analysis of cancer cell populations isolated from routine, formalin-fixed, paraffin-embedded tissue samples. Comparison of copy number changes detected by degenerate oligonucleotide-primed-polymerase chain reaction-based array CGH to those detected by conventional array CGH ...

  12. VizieR Online Data Catalog: Spectroscopy of main-belt Ch/Cgh-type asteroids (Vernazza+, 2016)

    Science.gov (United States)

    Vernazza, P.; Marsset, M.; Beck, P.; Binzel, R. P.; Birlan, M.; Cloutis, E. A.; DeMeo, F. E.; Dumas, C.; Hiroi, T.

    2016-09-01

    We conducted an extensive spectroscopic survey in the near-infrared range of 70 main-belt Ch/Cgh-type asteroids and 4 Ch/Cgh-type families and combined these measurements with available visible wavelength spectra. New data presented here are near-infrared asteroid spectral measurements for Ch- and Cgh-type asteroids from 0.7-2.5μm obtained using SpeX, the low- to medium-resolution near-IR spectrograph and imager on the 3m NASA InfraRed Telescope Facility (IRTF) located on Mauna Kea, HI. Observing runs were conducted remotely primarily from the Observatory of Paris-Meudon, France between 2010 April and 2012 January. The spectrograph SpeX, combined with a 0.8*15arcsec slit, was used in the low-resolution prism mode for acquisition of the spectra in the 0.7-2.5μm wavelength range. In order to monitor the high luminosity and variability of the sky in the near-IR, the telescope was moved along the slit during the acquisition of the data so as to obtain a sequence of spectra located at two different positions (A and B) on the array. In addition, we complemented our data set with additional near-infrared spectra retrieved from the Small Main-Belt Asteroid Spectroscopic Survey (SMASS) database (http://smass.mit.edu/). Combining these near-infrared measurements with available visible wavelength spectra (Bus, 1999PhDT........50B; Lazzaro et al., 2004Icar..172..179L) allows for the first time an extensive visible and near-infrared (VNIR) spectral database of main-belt Ch and Cgh types with D>45km (78% or 49/63 of all Ch and Cgh types listed in SMASS; see Table1). (1 data file).

  13. DNA copy number aberrations in breast cancer by array comparative genomic hybridization

    DEFF Research Database (Denmark)

    Li, J.; Wang, K.; Li, S.;

    2009-01-01

    Array comparative genomic hybridization (CGH) has been popularly used for analyzing DNA copy number variations in diseases like cancer. In this study, we investigated 82 sporadic samples from 49 breast cancer patients using 1-Mb resolution bacterial artificial chromosome CGH arrays. A number...

  14. Thermal design of spacecraft solar arrays using a polyimide foam

    Science.gov (United States)

    Bianco, N.; Iasiello, M.; Naso, V.

    2015-11-01

    The design of the Thermal Control System (TCS) of spacecraft solar arrays plays a fundamental role. Indeed, the spacecraft components must operate within a certain range of temperature. If this doesn't occur, their performance is reduced and they may even break. Solar arrays, which are employed to recharge batteries, are directly exposed to the solar heat flux, and they need to be insulated from the earth's surface irradiation. Insulation is currently provided either with a white paint coating or with a Multi Layer Insulation (MLI) system [1]. A configuration based on an open-cell polyimide foam has also been recently proposed [2]. Using polyimide foams in TCSs looks very attractive in terms of costs, weight and assembling. An innovative thermal analysis of the above cited TCS configurations is carried out in this paper, by solving the porous media energy equation, under the assumption of Local Thermal Equilibrium (LTE) between the two phases. Radiation effects through the solar array are also considered by using the Rosseland approximation. Under a stationary daylight condition, temperature profiles are obtained by means of the finite-element based code COMSOL Multiphysics®. Finally, since the weight plays an important role in aerospace applications, weights of the three TCS configurations are compared.

  15. SEPS solar array design and technology evaluation. [Solar Electric Propulsion Stage

    Science.gov (United States)

    Elms, R. V., Jr.; Young, L. E.

    1975-01-01

    The solar array system considered is composed of two wings. Each wing consists of a solar array blanket, a blanket launch storage container, an extension/retraction mast assembly, a blanket tensioning system, and an array electrical harness. A technology evaluation is performed to assess the applicable solar array state-of-the-art and to define the supporting effort necessary to achieve technology readiness for meeting the Solar Electric Propulsion Stage (SEPS) solar array design requirements. Details of mechanical design are discussed along with questions of electrical design, operational reliability advantages, and array assembly advantages.

  16. Design of a focal plane array with analog neural preprocessing

    Science.gov (United States)

    Koren, Ivo; Dohndorf, Juergen; Schluessler, Jens-Uwe; Werner, Joerg; Kroenig, Arndt; Ramacher, Ulrich

    1996-12-01

    The design of a CMOS focal plane array with 128 by 128 pixels and analog neural preprocessing is presented. Optical input to the array is provided by substrate-well photodiodes. A two-dimensional neural grid wIth next- neighbor connectivity, implemented as differential current- mode circuit, is capable of spatial low-pass filtering combined with contrast enhancement or binarization. The gain, spatial filter and nonlinearity parameters of the neural network are controlled externally using analog currents. This allows the multipliers and sigmoid transducers to be operated in weak inversion for a wide parameter sweep range as well as in moderate or strong inversion for a larger signal to pattern-noise ratio. The cell outputs are sequentially read out by an offset compensated differential switched-capacitor multiplexer with column preamplifiers. The analog output buffer is designed for pixel rates up to 1 pixel/microsecond and 2 by 100 pF load capacitance. All digital clocks controlling the analog data path are generated on-chip. The clock timing is programmable via a serial computer interface. Using 1 micrometer double-poly double-metal CMOS process, one pixel cell occupies 96 by 96 micrometer2 and the total chip size is about 2.3 cm2. Operating the neural network in weak inversion, the power dissipation of the analog circuitry is less than 100 mW.

  17. Improved analysis of bacterial CGH data beyond the log-ratio paradigm

    Directory of Open Access Journals (Sweden)

    Aakra Ågot

    2009-03-01

    Full Text Available Abstract Background Existing methods for analyzing bacterial CGH data from two-color arrays are based on log-ratios only, a paradigm inherited from expression studies. We propose an alternative approach, where microarray signals are used in a different way and sequence identity is predicted using a supervised learning approach. Results A data set containing 32 hybridizations of sequenced versus sequenced genomes have been used to test and compare methods. A ROC-analysis has been performed to illustrate the ability to rank probes with respect to Present/Absent calls. Classification into Present and Absent is compared with that of a gaussian mixture model. Conclusion The results indicate our proposed method is an improvement of existing methods with respect to ranking and classification of probes, especially for multi-genome arrays.

  18. Design of Linear Systolic Arrays for Matrix Multiplication

    Directory of Open Access Journals (Sweden)

    MILOVANOVIC, E. I.

    2014-02-01

    Full Text Available This paper presents architecture for matrix multiplication optimized to be integrated as an accelerator unit to a host computer. Two linear systolic arrays with unidirectional data flow (ULSA, used as hardware accelerators, where synthesized in this paper. The solution proposed here is designed to accelerate both the computation and communication by employing hardware address generator units (AGUs. The proposed design has been implemented on Xilinx Spartan-2E and Virtex4 FPGAs. In order to evaluate performance of the proposed solution, we have introduced quantitative and qualitative performance criteria. For the ULSA with n processing elements (PEs, the speed-up is O(n/2. Average gain factor of hardware AGUs is about 2.7, with hardware overhead of 0.6% for 32-bit PEs.

  19. Design and Experiment of Multi-resolution Composite Digital Array Antenna

    Directory of Open Access Journals (Sweden)

    Tang Yue

    2016-06-01

    Full Text Available Because a sparse array has the advantages of a simplified structure and reduced cost in a radar system, radar technology based on the sparse array has gained widespread attention. To take advantage of the sparse array, in this paper, we designed a Multi-Resolution Composite digital Array antenna (MRCA, and then used it in single-target and multi-target detection experiments. Using the same number of array elements, our experimental results demonstrate that the MRCA can obtain a narrower main lobe and a lower side lobe, enhances the direction of the array antenna, and improves the angular resolution compared with the uniform linear array.

  20. Design and Realization of Array Signal Processor VLSI Architecture for Phased Array System

    Directory of Open Access Journals (Sweden)

    D. Govind Rao

    2016-08-01

    Full Text Available A method for implementing an array signal processor for phased array radars. The array signal processor can receive planar array antenna inputs and can process it. It is based on the application of Adaptive Digital beam formers using FPGAs. Adaptive filter algorithm used here is Inverse Q-R Decomposition based Recursive Least Squares (IQRD-RLS [1] algorithm. Array signal processor based on FPGAs is suitable in the areas of Phased Array Radar receiver, where speed, accuracy and numerical stability are of utmost important. Using IQRD-RLS algorithm, optimal weights are calculated in much less time compared to conventional QRD-RLS algorithm. A customized multiple FPGA board comprising three Kintex-7 FPGAs is employed to implement array signal processor. The proposed architecture can form multiple beams from planar array antenna elements

  1. Structural design and analysis of a solar array substrate for a GEO satellite

    OpenAIRE

    Safak, Omer

    2013-01-01

    The aim of this thesis is the design of solar array substrate for a geostationary satellite. The design of deployable solar array substrate is realized based on the requirements which are provided by BILUZAY (Bilkent University Space Technologies Research Centre). This array is going to empower a telecommunication satellite which will be operating in a geostationary orbit during 15 years. The main work presented in this thesis consists of two principal directions: solar cell array area dimens...

  2. Statistical design of position-encoded microsphere arrays.

    Science.gov (United States)

    Sarder, Pinaki; Nehorai, Arye

    2011-03-01

    We propose a microsphere array device with microspheres having controllable positions for error-free target identification. We conduct a statistical design analysis to select the optimal distance between the microspheres as well as the optimal temperature. Our design simplifies the imaging and ensures a desired statistical performance for a given sensor cost. Specifically, we compute the posterior Cramér-Rao bound on the errors in estimating the unknown target concentrations. We use this performance bound to compute the optimal design variables. We discuss both uniform and sparse concentration levels of targets, and replace the unknown imaging parameters with their maximum likelihood estimates. We illustrate our design concept using numerical examples. The proposed microarray has high sensitivity, efficient packing, and guaranteed imaging performance. It simplifies the imaging analysis significantly by identifying targets based on the known positions of the microspheres. Potential applications include molecular recognition, specificity of targeting molecules, protein-protein dimerization, high throughput screening assays for enzyme inhibitors, drug discovery, and gene sequencing.

  3. Designing a Hemispherical Breast RF Coil Array for MRI

    Institute of Scientific and Technical Information of China (English)

    XU Wen-long; ZHANG Ju-cheng; WANG Zhi-kang; LI Xing-xin; LOU Hai-fang

    2015-01-01

    In this paper, an approach to the design of hemispherical breast RF coil array is proposedThe target field method is applied to find the current density distribution on the hemisphere surface, which induces a homogeneous magnetic field in a hemispherical volumeThe components of current density are expanded into Fourier series, and the highly ill-conditioned character of the linear equation related to Fourier coefficients is solved using the Tikhonov regularization method with a minimum curvature penalty functionThe winding pattern was acquired using the stream function techniqueThe results indicate that a simple winding pattern with homogeneous magnetic field can be obtained through manually selecting the penalty factor.

  4. Design optimization methods for genomic DNA tiling arrays.

    Science.gov (United States)

    Bertone, Paul; Trifonov, Valery; Rozowsky, Joel S; Schubert, Falk; Emanuelsson, Olof; Karro, John; Kao, Ming-Yang; Snyder, Michael; Gerstein, Mark

    2006-02-01

    A recent development in microarray research entails the unbiased coverage, or tiling, of genomic DNA for the large-scale identification of transcribed sequences and regulatory elements. A central issue in designing tiling arrays is that of arriving at a single-copy tile path, as significant sequence cross-hybridization can result from the presence of non-unique probes on the array. Due to the fragmentation of genomic DNA caused by the widespread distribution of repetitive elements, the problem of obtaining adequate sequence coverage increases with the sizes of subsequence tiles that are to be included in the design. This becomes increasingly problematic when considering complex eukaryotic genomes that contain many thousands of interspersed repeats. The general problem of sequence tiling can be framed as finding an optimal partitioning of non-repetitive subsequences over a prescribed range of tile sizes, on a DNA sequence comprising repetitive and non-repetitive regions. Exact solutions to the tiling problem become computationally infeasible when applied to large genomes, but successive optimizations are developed that allow their practical implementation. These include an efficient method for determining the degree of similarity of many oligonucleotide sequences over large genomes, and two algorithms for finding an optimal tile path composed of longer sequence tiles. The first algorithm, a dynamic programming approach, finds an optimal tiling in linear time and space; the second applies a heuristic search to reduce the space complexity to a constant requirement. A Web resource has also been developed, accessible at http://tiling.gersteinlab.org, to generate optimal tile paths from user-provided DNA sequences.

  5. Subjective assessments of spherical microphone arrays - Paired comparisons of two arrays designed using different microphone models

    OpenAIRE

    Koehl, Vincent; Paquier, Mathieu; Delikaris-Manias, Simeon

    2012-01-01

    International audience Microphone arrays are commonly used to capture sound fields. As the number of sensors forming the array increases, the spatial sampling accuracy at high frequencies improves. Numerous prototypes of spherical arrays were developed over the last years. However, much less attention has been paid to the intrinsic performances of the sensors than to their number and arrangement. This study aims at evaluating the relative performances of two rigid spherical microphone arra...

  6. Design of Circularly-Polarised, Crossed Drooping Dipole, Phased Array Antenna Using Genetic Algorithm Optimisation

    DEFF Research Database (Denmark)

    Larsen, Niels Vesterdal

    2007-01-01

    A printed drooping dipole array is designed and constructed. The design is based on a genetic algorithm optimisation procedure used in conjunction with the software programme AWAS. By optimising the array G/T for specific combinations of scan angles and frequencies an optimum design is obtained...

  7. Computer-generated holograms (CGH) realization: the integration of dedicated software tool with digital slides printer

    Science.gov (United States)

    Guarnieri, Vittorio; Francini, Franco

    1997-12-01

    Last generation of digital printer is usually characterized by a spatial resolution enough high to allow the designer to realize a binary CGH directly on a transparent film avoiding photographic reduction techniques. These devices are able to produce slides or offset prints. Furthermore, services supplied by commercial printing company provide an inexpensive method to rapidly verify the validity of the design by means of a test-and-trial process. Notably, this low-cost approach appears to be suitable for a didactical environment. On the basis of these considerations, a set of software tools able to design CGH's has been developed. The guidelines inspiring the work have been the following ones: (1) ray-tracing approach, considering the object to be reproduced as source of spherical waves; (2) Optimization and speed-up of the algorithms used, in order to produce a portable code, runnable on several hardware platforms. In this paper calculation methods to obtain some fundamental geometric functions (points, lines, curves) are described. Furthermore, by the juxtaposition of these primitives functions it is possible to produce the holograms of more complex objects. Many examples of generated CGHs are presented.

  8. Design of a 7kw power transfer solar array drive mechanism

    Energy Technology Data Exchange (ETDEWEB)

    Sheppard, J.G.

    1982-05-01

    With the availability of the Shuttle and the European launcher, Ariane, there will be a continuing trend towards large payload satellite missions requiring high-power, high-inertia, flexible solar arrays. The need arises for a solar array drive with a large power transfer capability which can rotate these solar arrays without disturbing the satellite body pointing. The modular design of such a Solar Array Drive Mechanism (SADM) which is capable of transferring 7kW of power or more is described. Total design flexibility has been achieved, enabling different spacecraft power requirements to be accommodated within the SADM design.

  9. Lightweight, Space and Power Efficient Solar Array Drive Design Implemented within Remote Interface Unit

    Science.gov (United States)

    Kivikyto, Tatu; Laaksoen, Jyrki

    2014-08-01

    PATRIA has implemented highly educated Remote Interface Units in various ESA missions. For the Sentinel-2 and EarthCARE it was constructed also to include Solar Array Drive Electronics. The same control design as for Solar Array Drive function was also piggybacked in Magneto Torque control drives and other mission specific stepper motor drives. The purpose of this paper is to summarise and present the PATRIA Solar Array Drive Electronics design advantages.

  10. MIMO radar arrays with minimum redundancy: a design method

    Science.gov (United States)

    Kirschner, A. J.; Siart, U.; Guetlein, J.; Detlefsen, J.

    2013-10-01

    Coherent multiple-input multiple-output (MIMO) radar systems with co-located antennas, form monostatic vir- tual arrays by discrete convolution of a bistatic setup of transmitters and receivers. Thereby, a trade-off between maximum array dimension, element spacing and hardware efforts exists. In terms of estimating the direction of arrival, the covariance matrix of the array element signals plays an important role. Here, minimum redundancy arrays aim at a hardware reduction with signal reconstruction by exploiting the Toeplitz characteristics of the covariance matrix. However, the discrete spatial convolution complicates the finding of an optimal antenna setup with minimum redundancy. Combinatorial effort is the consequence. This paper presents a possible simplified algorithm in order to find MIMO array setups of maximum dimension with minimum redundancy.

  11. Mechanical design and development of TES bolometer detector arrays for the Advanced ACTPol experiment

    CERN Document Server

    Ward, Jonathan T; Beall, James A; Choi, Steve K; Crowley, Kevin T; Devlin, Mark J; Duff, Shannon M; Gallardo, Patricio M; Henderson, Shawn W; Ho, Shuay-Pwu Patty; Hilton, Gene; Hubmayr, Johannes; Khavari, Niloufar; Klein, Jeffrey; Koopman, Brian J; Li, Dale; McMahon, Jeffrey; Mumby, Grace; Nati, Federico; Niemack, Michael D; Page, Lyman A; Salatino, Maria; Schillaci, Alessandro; Schmitt, Benjamin L; Simon, Sara M; Staggs, Suzanne T; Thornton, Robert; Ullom, Joel N; Vavagiakis, Eve M; Wollack, Edward J

    2016-01-01

    The next generation Advanced ACTPol (AdvACT) experiment is currently underway and will consist of four Transition Edge Sensor (TES) bolometer arrays, with three operating together, totaling ~5800 detectors on the sky. Building on experience gained with the ACTPol detector arrays, AdvACT will utilize various new technologies, including 150mm detector wafers equipped with multichroic pixels, allowing for a more densely packed focal plane. Each set of detectors includes a feedhorn array of stacked silicon wafers which form a spline profile leading to each pixel. This is then followed by a waveguide interface plate, detector wafer, back short cavity plate, and backshort cap. Each array is housed in a custom designed structure manufactured from high purity copper and then gold plated. In addition to the detector array assembly, the array package also encloses cryogenic readout electronics. We present the full mechanical design of the AdvACT high frequency (HF) detector array package along with a detailed look at t...

  12. AN ALTERNATIVE APPROACH TO DESIGN AND ANALYSIS OF BROADBAND BEAMSPACE ADAPTIVE ARRAYS

    Directory of Open Access Journals (Sweden)

    A. S. SRINIVASA RAO

    2011-09-01

    Full Text Available The beamwidth of a linear array depends on number of elements in the array and frequency of the input signal. At present designing of wideband antennas and beamformers became important, in the fields of microphone arrays intended for teleconferencing, in transmitting or receiving spread spectrum signals, crip signals etc. A beamspace adaptive planar array for broadband beamforming is proposed based on the filter – and - sum beamforming technique. A detailed design method was provided for both the linear arrays and the adaptivearrays and simulation results are provided for the proposed method. Our proposed method is used to demonstrate that the beam-space adaptive array can suppress interference signals having a wide fractional bandwidth and that the array has fast convergence.

  13. A web server for mining Comparative Genomic Hybridization (CGH) data

    Science.gov (United States)

    Liu, Jun; Ranka, Sanjay; Kahveci, Tamer

    2007-11-01

    Advances in cytogenetics and molecular biology has established that chromosomal alterations are critical in the pathogenesis of human cancer. Recurrent chromosomal alterations provide cytological and molecular markers for the diagnosis and prognosis of disease. They also facilitate the identification of genes that are important in carcinogenesis, which in the future may help in the development of targeted therapy. A large amount of publicly available cancer genetic data is now available and it is growing. There is a need for public domain tools that allow users to analyze their data and visualize the results. This chapter describes a web based software tool that will allow researchers to analyze and visualize Comparative Genomic Hybridization (CGH) datasets. It employs novel data mining methodologies for clustering and classification of CGH datasets as well as algorithms for identifying important markers (small set of genomic intervals with aberrations) that are potentially cancer signatures. The developed software will help in understanding the relationships between genomic aberrations and cancer types.

  14. EM design and analysis of dipole arrays on non-planar dielectric substrate

    CERN Document Server

    Singh, Hema; Jha, Rakesh Mohan

    2016-01-01

    This book presents a simple and systematic description of EM design of antenna arrays. Printed dipole antennas are known to be simple yet more efficient than wire antennas. The dielectric substrate and the presence of ground plane affect the antenna performance and the resonant frequency is shifted. This book includes the EM design and performance analysis of printed dipole arrays on planar and cylindrical substrates. The antenna element is taken as half-wave centre-fed dipole. The substrate is taken as low-loss dielectric. The effect of substrate material, ground plane, and the curvature effect is discussed. Results are presented for both the linear and planar dipole arrays. The performance of dipole array is analyzed in terms of input impedance, return loss, and radiation pattern for different configurations. The effect of curved platform (substrate and ground plane) on the radiation behaviour of dipole array is analyzed. The book explains fundamentals of EM design and analysis of dipole antenna array throu...

  15. Design of a low-profile printed array of loaded dipoles with inherent frequency selectivity properties

    NARCIS (Netherlands)

    Cavallo, D.; Savoia, S.; Gerini, G.; Neto, A.; Galdi, V.

    2011-01-01

    This work presents the design of a low-profile array of printed dipoles with inherent filtering properties for radar applications. The antenna and the band-pass filter are integrated in a single module, which is small enough to fit within the array unit cell (with period of about 0.4 ? at the highes

  16. Design and Realization of Phased Array Radar Optical Fiber Transmission System

    Institute of Scientific and Technical Information of China (English)

    HU Shan-qing; LIU Feng; LONG Teng

    2007-01-01

    One optical fiber transmission system is designed.The modularization optical fiber transmission adapters were utilized in the system,so the system structure could be flexibly scalable.The sub-array adapter and signal processor adapter were designed and realized utilizing the new field programmable gate array (FPGA) which could drive the optical transceiver.The transmission agreement was designed based on the data stream.In order to solve the signal synchronization problem of the optical fiber transmitted phased array radar,a method named synchronous clock was designed.The fiber transmission error code rate of the system was zero with an experimental transmission velocity of 800 Mbit/s.The phased array radar system has detected the airplane target,thus validated the feasibility of the design method.

  17. A head and neck hyperthermia applicator: Theoretical antenna array design

    NARCIS (Netherlands)

    Paulides, M.M.; Bakker, J.F.; Zwamborn, A.P.M.; Rhoon, G.C. van

    2007-01-01

    Purpose: Investigation into the feasibility of a circular array of dipole antennas to deposit RF-energy centrally in the neck as a function of: 1) patient positioning, 2) antenna ring radius, 3) number of antenna rings, 4) number of antennas per ring and 5) distance between antenna rings. Materials

  18. A head and neck hyperthermia applicator: Theoretical antenna array design

    NARCIS (Netherlands)

    M.M. Paulides (Margarethus); J.F. Bakker (Jurriaan); A.P.M. Zwamborn; G.C. van Rhoon (Gerard)

    2007-01-01

    textabstractPurpose: Investigation into the feasibility of a circular array of dipole antennas to deposit RF-energy centrally in the neck as a function of: (1) patient positioning, (2) antenna ring radius, (3) number of antenna rings, (4) number of antennas per ring and (5) distance between antenna

  19. ICE decoupling technique for RF coil array designs

    OpenAIRE

    Li, Ye; Xie, Zhentian; Pang, Yong; Vigneron, Daniel; Zhang, Xiaoliang

    2011-01-01

    Purpose: Parallel magnetic resonance imaging (MRI) requires an array of RF coil elements with different sensitivity distributions and with minimal electromagnetic coupling. The goal of this project was to develop a new method based on induced current compensation or elimination (ICE) for improved coil element decoupling and to investigate its performance in phantom MR images.

  20. Optimized design and assessment of whole genome tiling arrays.

    NARCIS (Netherlands)

    Graf, S.; Nielsen, F.G.G.; Kurtz, S.; Huynen, M.A.; Birney, E.; Stunnenberg, H.G.; Flicek, P.

    2007-01-01

    MOTIVATION: Recent advances in microarray technologies have made it feasible to interrogate whole genomes with tiling arrays and this technique is rapidly becoming one of the most important high-throughput functional genomics assays. For large mammalian genomes, analyzing oligonucleotide tiling arra

  1. Coherent MIMO Array Design With Periodical Physical Element Structures

    NARCIS (Netherlands)

    Belfiori, F.; Rossum, W.L. van; Peter Hoogeboom, P.

    2011-01-01

    Multiple-input-multiple-output (MIMO) processing is a consolidated technique in communication systems thanks to the benefits that it offers in multipath fading environments. In recent years, studies have shown how the performances of conventional phased-array radar can be improved by using the same

  2. Genome-wide array-based comparative genomic hybridization (array-CGH) analysis in Aicardi Syndrome

    Science.gov (United States)

    Aicardi syndrome is characterized by agenesis of the corpus callosum, chorioretinal lacunae, severe seizures (starting as infantile spasms), neuronal migration defects, mental retardation, costovertebral defects, and typical facial features. Because Aicardi syndrome is sporadic and affects only fem...

  3. Mechanical Design and Development of TES Bolometer Detector Arrays for the Advanced ACTPol Experiment

    Science.gov (United States)

    Ward, Jonathan T.; Austermann, Jason; Beall, James A.; Choi, Steve K.; Crowley, Kevin T.; Devlin, Mark J.; Duff, Shannon M.; Gallardo, Patricio M.; Henderson, Shawn W.; Ho, Shuay-Pwu Patty; Hilton, Gene; Hubmayr, Johannes; Khavari, Niloufar; Klein, Jeffrey; Koopman, Brian J.; Li, Dale; McMahon, Jeffrey; Mumby, Grace; Nati, Federico; Wollack, Edward J.

    2016-01-01

    The next generation Advanced ACTPol (AdvACT) experiment is currently underway and will consist of four Transition Edge Sensor (TES) bolometer arrays, with three operating together, totaling 5800 detectors on the sky. Building on experience gained with the ACTPol detector arrays, AdvACT will utilize various new technologies, including 150 mm detector wafers equipped with multichroic pixels, allowing for a more densely packed focal plane. Each set of detectors includes a feedhorn array of stacked silicon wafers which form a spline pro le leading to each pixel. This is then followed by a waveguide interface plate, detector wafer, back short cavity plate, and backshort cap. Each array is housed in a custom designed structure manufactured from high purity copper and then gold plated. In addition to the detector array assembly, the array package also encloses cryogenic readout electronics. We present the full mechanical design of the AdvACT high frequency (HF) detector array package along with a detailed look at the detector array stack assemblies. This experiment will also make use of extensive hardware and software previously developed for ACT, which will be modi ed to incorporate the new AdvACT instruments. Therefore, we discuss the integration of all AdvACT arrays with pre-existing ACTPol infrastructure.

  4. Design of Optimal Directional Parametric Acoustic Arrays in Air

    Science.gov (United States)

    Garner, Glenwood, III

    The nonlinear interaction of propagating acoustic waves in air is exploited as a means of generating highly directional sound beams at both audio frequencies and ultrasonic frequencies. A solution for the third-order intermodulation (IM3) products generated by the nonlinearity of air with a two-tone source waveform is derived by using cascaded secondorder nonlinearities. This method is applied to collinear sources as well as noncollinear sources. With collinear sources, the third-order interaction of two ultrasonic primary tones, where one is approximately the second harmonic of the other, produces an IM3 tone in the audio band. This IM3 tone is shown both theoretically and experimentally to have greater directivity than the difference tone produced by a traditional parametric array which utilizes second-order effects. With noncollinear sources, the third-order interaction of two ultrasonic primary tones closely spaced in frequency produces IM3 upper and IM3 lower tones that are also closely spaced in frequency to the primary tones. The IM3 upper and IM3 lower tones are shown both theoretically and experimentally to have greater directivity than either of the primary tones. Third-order intermodulation distortion generated by the electronic amplifiers and piezoelectric transducers is also shown to contribute to the third-order intermodulation products generated by air. The nonlinear contributions of the amplifier and transducer are modeled using power series expansions and measurements are performed to determine the power series coefficients. A new geometry for the optimum placement of ultrasonic transducers in a multi-element array is derived using the genetic algorithm and results in sparsely packed transducer elements. For primary ultrasonic tones closely spaced in frequency, the sparsely packed array is shown both theoretically and experimentally to produce a difference tone with greater directivity than obtained with a traditional densely packed hexagonal array

  5. Miniaturization Smart Antenna Array Design for TD-SCDMA System

    Directory of Open Access Journals (Sweden)

    Hao Honggang

    2013-09-01

    Full Text Available In view of the development for TD-SCDMA communication system and combine with the miniaturization technology of smart antenna, by using balun feeding structure and consist of splitters and neural networks,  this paper  proposed a four units of dual polarized and a eight units of +45o/-45o polarized antenna array which on the basis of printed dipole antenna. The simulation results show that the isolation of dual-polarized unit between two ports is greater than -26dB, the isolation of 4-unit dual-polarized antenna array between dipole ports are less than -20dB and the 8-unit is less than -18dB. The polarization characteristics and isolation parameter of the proposed antenna has meets the demands of practical applications in the bands of TD-SCDMA communication system and its supplement bands.

  6. Design and Simulation of High-power LED Array Packaging

    Institute of Scientific and Technical Information of China (English)

    TIAN Da-lei; GUAN Rong-feng; WANG Xing

    2008-01-01

    Thermal management is one of the key technologies for high-power Light emitting diode(LED) entering into the general illuminating field. Successful thermal management depends on optimal packaging structure and selected packaging materials. In this paper, the aluminum is employed as a substrate of LED, 3×3 array chips are placed on the substrate, heat dissipation performance is simulated using finite element analysis(FEA) software, analyzed are the influences on the temperature of the chip with different convection coefficient, and optical properties are simulated using optical analysis software. The results show that the packaging structure can not only effectually improve the thermal performance of high-power LED array but also increase the light extraction efficiency.

  7. Near-unity broadband absorption designs for semiconducting nanowire arrays via localized radial mode excitation.

    Science.gov (United States)

    Fountaine, Katherine T; Kendall, Christian G; Atwater, Harry A

    2014-05-01

    We report design methods for achieving near-unity broadband light absorption in sparse nanowire arrays, illustrated by results for visible absorption in GaAs nanowires on Si substrates. Sparse (unity absorption at wire resonant wavelengths due to coupling into 'leaky' radial waveguide modes of individual wires and wire-wire scattering processes. From a detailed conceptual development of radial mode resonant absorption, we demonstrate two specific geometric design approaches to achieve near unity broadband light absorption in sparse nanowire arrays: (i) introducing multiple wire radii within a small unit cell array to increase the number of resonant wavelengths, yielding a 15% absorption enhancement relative to a uniform nanowire array and (ii) tapering of nanowires to introduce a continuum of diameters and thus resonant wavelengths excited within a single wire, yielding an 18% absorption enhancement over a uniform nanowire array.

  8. Resolving phase ambiguities in the calibration of redundant interferometric arrays: implications for array design

    Science.gov (United States)

    Kurien, Binoy G.; Tarokh, Vahid; Rachlin, Yaron; Shah, Vinay N.; Ashcom, Jonathan B.

    2016-10-01

    We provide new results enabling robust interferometric image reconstruction in the presence of unknown aperture piston variation via the technique of redundant spacing calibration (RSC). The RSC technique uses redundant measurements of the same interferometric baseline with different pairs of apertures to reveal the piston variation among these pairs. In both optical and radio interferometry, the presence of phase-wrapping ambiguities in the measurements is a fundamental issue that needs to be addressed for reliable image reconstruction. In this paper, we show that these ambiguities affect recently developed RSC phasor-based reconstruction approaches operating on the complex visibilities, as well as traditional phase-based approaches operating on their logarithm. We also derive new sufficient conditions for an interferometric array to be immune to these ambiguities in the sense that their effect can be rendered benign in image reconstruction. This property, which we call wrap-invariance, has implications for the reliability of imaging via classical three-baseline phase closures as well as generalized closures. We show that wrap-invariance is conferred upon arrays whose interferometric graph satisfies a certain cycle-free condition. For cases in which this condition is not satisfied, a simple algorithm is provided for identifying those graph cycles which prevent its satisfaction. We apply this algorithm to diagnose and correct a member of a pattern family popular in the literature.

  9. Design and Performance of the ARIANNA Hexagonal Radio Array Systems

    CERN Document Server

    Barwick, S W; Besson, D Z; Cheim, E; Duffin, T; Hanson, J C; Klein, S R; Kleinfelder, S A; Prakash, T; Piasecki, M; Ratzlaff, K; Reed, C; Roumi, M; Samanta, A; Stezelberger, T; Tatar, J; Walker, J; Young, R; Zou, L

    2014-01-01

    We report on the development, installation and operation of the first three of seven stations deployed at the ARIANNA site's pilot Hexagonal Radio Array in Antarctica. The primary goal of the ARIANNA project is to observe ultra-high energy (>100 PeV) cosmogenic neutrino signatures using a large array of autonomous stations each dispersed 1 km apart on the surface of the Ross Ice Shelf. Sensing radio emissions of 100 MHz to 1 GHz, each station in the array contains RF antennas, amplifiers, 1.92 G-sample/s, 850 MHz bandwidth signal acquisition circuitry, pattern-matching trigger capabilities, an embedded CPU, 32 GB of solid-state data storage, and long-distance wireless and satellite communications. Power is provided by the sun and LiFePO4 storage batteries, and the stations consume an average of 7W of power. Operation on solar power has resulted in >=58% per calendar-year live-time. The station's pattern-trigger capabilities reduce the trigger rates to a few milli-Hertz with 4-sigma thresholds while retaining ...

  10. Proceedings of the Flat-Plate Solar Array Project Research Forum on the Design of Flat-Plate Photovoltaic Arrays for Central Stations

    Science.gov (United States)

    The Flat Plate Solar Array Project, focuses on advancing technologies relevant to the design and construction of megawatt level central station systems. Photovoltaic modules and arrays for flat plate central station or other large scale electric power production facilities require the establishment of a technical base that resolves design issues and results in practical and cost effective configurations. Design, qualification and maintenance issues related to central station arrays derived from the engineering and operating experiences of early applications and parallel laboratory reserch activities are investigated. Technical issues are examined from the viewpoint of the utility engineer, architect/engineer and laboratory researcher. Topics on optimum source circuit designs, module insulation design for high system voltages, array safety, structural interface design, measurements, and array operation and maintenance are discussed.

  11. Corporate-Feed Multilayer Bow-Tie Antenna Array Design Using a Simple Transmission Line Model

    OpenAIRE

    Didouh, S.; Abri, M.; F. T. Bendimerad

    2012-01-01

    A transmission line model is used to design corporate-fed multilayered bow-tie antennas arrays; the simulated antennas arrays are designed to resonate at the frequencies 2.4 GHz, 5 GHz, and 8 GHz corresponding to RFID, WIFI, and radars applications. The contribution of this paper consists of modeling multilayer bow-tie antenna array fed through an aperture using transmission line model. The transmission line model is simple and precise and allows taking into account the whole geometrical, ele...

  12. Low power and reliable SRAM memory cell and array design

    CERN Document Server

    Ishibashi, Koichiro

    2011-01-01

    Success in the development of recent advanced semiconductor device technologies is due to the success of SRAM memory cells. This book addresses various issues for designing SRAM memory cells for advanced CMOS technology. To study LSI design, SRAM cell design is the best materials subject because issues about variability, leakage and reliability have to be taken into account for the design.

  13. Fast Computation of Wideband Beam Pattern for Designing Large-Scale 2-D Arrays.

    Science.gov (United States)

    Chi, Cheng; Li, Zhaohui

    2016-06-01

    For real-time and high-resolution 3-D ultrasound imaging, the design of sparse distribution and weights of elements of a large-scale wideband 2-D array is needed to reduce hardware cost and achieve better directivity. However, due to the high time consumption of computing the wideband beam pattern, the design methods that need massive iterations have rarely been applied to design large-scale wideband 2-D arrays by directly computing the wideband beam pattern. In this paper, a fast method is proposed to realize the computation of a wideband beam pattern of arbitrary 2-D arrays in the far field in order to design large-scale wideband 2-D arrays. The proposed fast method exploits two important techniques: 1) nonuniform fast Fourier transform (FFT) and 2) short inverse FFT. Compared with the commonly used ultrasound simulator Field II, two orders of magnitude improvement in computation speed is achieved with comparable accuracy. The proposed fast method enables massive iterations of direct wideband beam pattern computation of arbitrary large-scale 2-D arrays. A design example in this paper demonstrates that the proposed fast method can help achieve better performance in designing large-scale wideband 2-D arrays. PMID:27046870

  14. Design of an inflatable, optically controlled and fed, phased array antenna

    Science.gov (United States)

    Kunath, Richard R.; Sharp, G. R.

    1991-09-01

    Initial studies on the antenna requirements of the Space Exploration Initiative (SEI) system architecture have indicated the need for large, lightweight antennas. This paper discusses the design of a modular, inflatable, optically controlled and fed phased array antenna suitable for SEI aplications. When high gain antennas are required for space applications, large aperture mesh or collapsible solid antenna reflectors are considered. However, these designs are generally not lightweight, and have complicated deployment mechanisms. Alternatively, the modular, inflatable antenna design discussed here is a lightweight, modular design that incorporates a simple deployment scheme, and after deployment, can be rigidized to enhance its structural integrity. Further, the design features the fiberoptic distribution of both RF and control signals to individual microwave integrated circuit/reflector modules in each of the inflatable, phased array antenna cells. The result of combining these two technologies is a modular, phased array antenna design that is both mechanically and electrically agile and robust.

  15. Design, processing and testing of LSI arrays, hybrid microelectronics task

    Science.gov (United States)

    Himmel, R. P.; Stuhlbarg, S. M.; Ravetti, R. G.; Zulueta, P. J.; Rothrock, C. W.

    1979-01-01

    Mathematical cost models previously developed for hybrid microelectronic subsystems were refined and expanded. Rework terms related to substrate fabrication, nonrecurring developmental and manufacturing operations, and prototype production are included. Sample computer programs were written to demonstrate hybrid microelectric applications of these cost models. Computer programs were generated to calculate and analyze values for the total microelectronics costs. Large scale integrated (LST) chips utilizing tape chip carrier technology were studied. The feasibility of interconnecting arrays of LSU chips utilizing tape chip carrier and semiautomatic wire bonding technology was demonstrated.

  16. Designing of sparse 2D arrays for Lamb wave imaging using coarray concept

    Energy Technology Data Exchange (ETDEWEB)

    Ambroziński, Łukasz, E-mail: ambrozin@agh.edu.pl; Stepinski, Tadeusz, E-mail: ambrozin@agh.edu.pl; Uhl, Tadeusz, E-mail: ambrozin@agh.edu.pl [AGH University of Science and technology, al. Mickiewicza 30, 30-059 Krakow (Poland)

    2015-03-31

    2D ultrasonic arrays have considerable application potential in Lamb wave based SHM systems, since they enable equivocal damage imaging and even in some cases wave-mode selection. Recently, it has been shown that the 2D arrays can be used in SHM applications in a synthetic focusing (SF) mode, which is much more effective than the classical phase array mode commonly used in NDT. The SF mode assumes a single element excitation of subsequent transmitters and off-line processing the acquired data. In the simplest implementation of the technique, only single multiplexed input and output channels are required, which results in significant hardware simplification. Application of the SF mode for 2D arrays creates additional degrees of freedom during the design of the array topology, which complicates the array design process, however, it enables sparse array designs with performance similar to that of the fully populated dense arrays. In this paper we present the coarray concept to facilitate synthesis process of an array’s aperture used in the multistatic synthetic focusing approach in Lamb waves-based imaging systems. In the coherent imaging, performed in the transmit/receive mode, the sum coarray is a morphological convolution of the transmit/receive sub-arrays. It can be calculated as the set of sums of the individual sub-arrays’ elements locations. The coarray framework will be presented here using a an example of a star-shaped array. The approach will be discussed in terms of beampatterns of the resulting imaging systems. Both simulated and experimental results will be included.

  17. Predictive Array Design. A method for sampling combinatorial chemistry library space.

    Science.gov (United States)

    Lipkin, M J; Rose, V S; Wood, J

    2002-01-01

    A method, Predictive Array Design, is presented for sampling combinatorial chemistry space and selecting a subarray for synthesis based on the experimental design method of Latin Squares. The method is appropriate for libraries with three sites of variation. Libraries with four sites of variation can be designed using the Graeco-Latin Square. Simulated annealing is used to optimise the physicochemical property profile of the sub-array. The sub-array can be used to make predictions of the activity of compounds in the all combinations array if we assume each monomer has a relatively constant contribution to activity and that the activity of a compound is composed of the sum of the activities of its constitutive monomers.

  18. Cost Efficient Design Approach for Reversible Programmable Logic Arrays

    OpenAIRE

    Md. RiazurRahman

    2016-01-01

    Reversible programmable logicarrays (PLA) are at the heart of designing of efficient low power computers. This paper presents anefficient approach to design Reversible PLAs that maximizes the usability of garbage outputs and also reduces the number of ancilla inputs generated. The designfor proposed essentialcomponents and the architecture of reversible grid network for designing AND and EX-OR planes are also presented. Several algorithms have been proposed and presented to describe th...

  19. Cost Efficient Design Approach for Reversible Programmable Logic Arrays

    Directory of Open Access Journals (Sweden)

    Md. RiazurRahman

    2016-06-01

    Full Text Available Reversible programmable logicarrays (PLA are at the heart of designing of efficient low power computers. This paper presents anefficient approach to design Reversible PLAs that maximizes the usability of garbage outputs and also reduces the number of ancilla inputs generated. The designfor proposed essentialcomponents and the architecture of reversible grid network for designing AND and EX-OR planes are also presented. Several algorithms have been proposed and presented to describe the programming interfaces in context of Reversible PLAs construction. Lastly, recent result on the trade-off between cost factors of standard benchmarkcircuits shows that the proposed design clearly outperforms the existing ones in terms various cost factors.

  20. The Bepi Colombo Mercury Transfer Module And Mercury Planetary Orbiter Solar Array Design And Development

    Science.gov (United States)

    Petry, D.; Grunwald, C.; Lohberg, A.; Brandi, A.; Oxynos-Lauschke, C.; Andreev, T.; Van Der Ven, R.; Schuhmacher, U.; Fugger, S.; Caon, A.; Fiebrich, H. K.

    2011-10-01

    The Bepi Colombo Mercury Transfer Module (MTM) and Mercury Planetary Orbiter (MPO) solar arrays are being designedto guaranteethe operational requirements in a very severe environment, mainly characterized by high temperatures (up to 215°C including qualification margin) and high light intensity (up to 11 solar constants). Furthermore, the qualification of the MPO solar array requires 5000 thermal cycles between -165°C and 215°C. The final configurations of the solar arrays are the result of a very complex system analysis aiming at reducing the solar array operative temperatures. The outcome of these specific developments performed on the photovoltaic assembly and panel substrate materials are essential drivers of the solar array design activity. The MTM solar array is composed by two wings of five solar panels and provides a peak power of about 13000W during the 6-years cruise phase to Mercury. The MPO solar generator is composed of one wing of three panels and provides an average power up to about 1600W during the nominal 1 earth year mission around Mercury. The photovoltaic assembly of both solar arrays is based on newly designed GaAs triple-junction solar cells. New carbon fibre reinforced Cyanate structures are applied as substrates.

  1. Design of a Compact Wideband Antenna Array for Microwave Imaging Applications

    Directory of Open Access Journals (Sweden)

    J. Puskely

    2013-12-01

    Full Text Available In the paper, wideband antenna arrays aimed at microwave imaging applications and SAR applications operating at Ka band were designed. The antenna array feeding network is realized by a low-loss SIW technology. Moreover, we have replaced the large feed network comprised of various T and Y junctions by a simple broadband network of compact size to more reduce losses in the substrate integrated waveguide and also save space on the PCB. The designed power 8-way divider is complemented by a wideband substrate integrated waveguide to a grounded coplanar waveguide transition and directly connected to the antenna elements. The measured results of antenna array are consistent with our simulation. Obtained results of the developed array demonstrated improvement compared to previously developed binary feed networks with microstrip or SIW splitters.

  2. Application of Novel Printed Dipole Antenna to Design Broadband Planar Phased Array

    Directory of Open Access Journals (Sweden)

    Jie Xu

    2014-01-01

    Full Text Available A broadband planar phased array consisting of 22 linear printed dipole antennas (PDA is presented in this paper. The element is designed by a coax probe feeding mechanism with a ground plate configuration. The PDA with two arms placed on both sides of a substrate is realized. The inner conductor of the coaxial cable is connected to the PDA’s upper arm, and the outer conductor is connected to the PDA’s lower arm, so it eliminates the balun. The impedance bandwidth of the PDA array can be improved by increasing mutual coupling effect between the adjacent array elements. A dielectric layer, which is integrated on the surface of the antenna, is designed and fabricated to improve the impedance bandwidth and to shield the array. The measured results indicate the active VSWR is less than 3 over the frequency range of 4–20 GHz.

  3. Design of Multilayer Dielectric Cover to Enhance Gain and Efficiency of Slot Arrays

    Directory of Open Access Journals (Sweden)

    Giorgio Montisci

    2013-01-01

    Full Text Available An effective design procedure, based on the Elliot synthesis method, is proposed to investigate the properties of waveguide slot arrays with multilayer dielectric cover. Then, the features of the designed arrays are analyzed by means of a FEM commercial software, namely, Ansys HFSS 13. We show how a proper choice of the dielectric cover configuration allows increasing the array gain and aperture efficiency, while taking advantage of the properties of the radome structure, in terms of insulation, protection, and pressurization of the radiating waveguides. Therefore, a significant outcome of the optimal choice of the multilayer dielectric cover is the reduction of the number of slots with respect to an array radiating into free space with the same gain and efficiency, with a consequent reduction of the production cost.

  4. Application of different spatial sampling patterns for sparse-array transducer design

    DEFF Research Database (Denmark)

    Nikolov, Svetoslav; Jensen, Jørgen Arendt

    2000-01-01

    In the last years the efforts of many researchers have been focused ondeveloping 3D real-time scanners. The use of 2D phased-array transducers makes it possible to steer the ultrasonicbeam in all directions in the scanned volume. An unacceptably large amount oftransducer channels (more than $4000...... of the ultrasound fields show a decrease of the grating-lobe level of 10 dB for the diagonally optimized 2D array transducers compared to the the previuosly designed 2D arrays which didn't consider the diagonals....

  5. Design and simulation of a tactile display based on a CMUT array

    Science.gov (United States)

    Chouvardas, Vasilios G.; Hatalis, Miltiadis K.; Miliou, Amalia N.

    2012-10-01

    In this article, we present the design of a tactile display based on a CMUT-phased array. The array implements a 'pixel' of the display and is used to focus airborne ultrasound energy on the skin surface. The pressure field, generated by the focused ultrasound waves, is used to excite the mechanoreceptors under the skin and transmit tactile information. The results of Finite Element Analysis (FEA) of the Capacitive Micromachined Ultrasonic Transducer (CMUT) and the CMUT-phased array for ultrasound emission are presented. The 3D models of the device and the array were developed using a commercial FEA package. Modelling and simulations were performed using the parameters from the POLYMUMPS surface micromachining technology from MEMSCAP. During the analysis of the phased array, several parameters were studied in order to determine their importance in the design of the tactile display. The output of the array is compared with the acoustic intensity thresholds in order to prove the feasibility of the design. Taking into account the density of the mechanoreceptors in the skin, we conclude that there should be at least one receptor under the excitation area formed on the skin.

  6. TPF-I Emma X-Array: 2007 Design Team Study

    Science.gov (United States)

    Martin, Stefan R.; Rodriguez, Jose; Scharf, Dan; Smith, Jim; McKinstry, David; Wirz, Richie; Purcell, George; Wayne, Len; Scherr, Larry; Mennesson, Bertrand; Lay, Oliver

    2007-01-01

    This viewgraph presentation is a study of an Emma design for Terrestrial Planet Finder (TPF) formation flying interferometer. The objective is to develop a design with reduced cost compared to TPF-I X-Array, derive mass and cost estimates, and study thermal and radiation issues.

  7. The design of a DAQ system for a GEM imaging detector based on FET array readout

    International Nuclear Information System (INIS)

    A data acquisition system was designed for a GEM imaging detector, which is readout by a FET switch array and can be used in real-time imaging. By using some advanced technologies, like FPGA and MCU, the designed DAQ system succeeds in multi-channel real-time readout with high-accuracy and high universality. (authors)

  8. CPV semi-dense array design for dish and tower collectors

    Science.gov (United States)

    Hayden, Herb; Thomas, Paul; Fette, Nicholas; Farkas, Zoltan; Bading, Michael; Stone, Bradley; Miner, Mark; Stickroth, Oliver; Bagewadi, Nakul; Romero, Memo; Sonuparlak, Birol; Eichholz, Rainer; Ziegler, Michael; Pawlowski, Edgar

    2012-10-01

    SST is developing a new Dish CPV dense array system that overcomes the flux uniformity requirement of previous designs. The ability to operate without flux uniformity relaxes the precision requirements of primary collector optics and eliminates homogenizing optics previously required for dense array CPV. Array design can be configured for dish and tower/heliostat systems developed for thermal CSP applications. The design uses industry standard CPV cells and manufacturing materials and methods for minimum cost and high reliability. Nominal input flux to the array for full power is about 250 suns. Internal array optics increase flux to the cells to about 1200 suns. Linear optics provide additional concentration, permit novel use of commercial glass production methods and facilitate power collection design that is integrated with dynamic power conversion and maximum power point tracking (MPPT). Efficient power hybrid packaging methods are used along with advanced liquid cooling "cold-plate" thermal management. Byproduct "waste heat" can be provided for on-site CHP use. We report on the design approach and status of development with the beginning of on-sun alpha testing of the first of 50 kW of CPV modules being produced.

  9. Congenital generalized hypertrichosis (CGH) maps to Xq26-q27

    Energy Technology Data Exchange (ETDEWEB)

    Figuera, L.E.; Dunne, P.W.; Pandolfo, M. [Baylor College of Medicine, Houston, TX (United States)] [and others

    1994-09-01

    CGH is a rare, X-linked dominant trait previously described by one of us in a large, five-generational Mexican family with 28 affected individuals. Family history and clinical examination reveal that excessive hair is present at the patient`s birth becoming more dense during the first year of life. In males the hair eventually covers the face and upper portion of the trunk. The affected women have transmitted the trait to both male and female offspring, while one affected male has transmitted the trait to all three female offspring but not to his nine sons. In addition, manifestations are more severe in males than females, who show an uneven pattern of excessive hair distribution, possibly due to the random nature of X-inactivation. The rarity of this trait and the apparently extremely low rate of mutation of the gene led the authors to hypothesize that this condition was the result of a {open_quotes}back{close_quotes} mutation, leading to reactivation of an {open_quotes}atavistic{close_quotes} gene. Clinical examination, blood collection, and establishment of lymphoblastoid cell lines have been completed for the majority of the members of the family available, including affected and unaffected males and females. Sixteen meioses were screened using several polymorphic microsatellite markers distributed along the X-chromosome. The locus DXS1211 did not show recombination events. Two-point linkage analysis yielded a maximum LOD score of 3.08 at theta of zero. An updated map of the X chromosome localizes this marker at Xq16-q27. The identification of the CGH gene will provide insight into development of hair and allow testing of the hypothesis of {open_quotes}atavism{close_quotes}.

  10. Identification and characterization of a de novo partial trisomy 10p by comparative genomic hybridization (CGH).

    Science.gov (United States)

    Benzacken, B; Lapierre, J M; Siffroi, J P; Chalvon, A; Tachdjian, G

    1998-10-01

    We report the characterization of a de novo unbalanced chromosome rearrangement by comparative genomic hybridization (CGH) in a 15-day-old child with hypotonia and dysmorphia. We describe the combined use of CGH and fluorescence in situ hybridization (FISH) to identify the origin of the additional chromosomal material on the short arm of chromosome 6. Investigation with FISH revealed that the excess material was not derived from chromosome 6. Identification of unknown unbalanced aberrations that could not be identified by traditional cytogenetics procedures is possible by CGH analysis. Visual analysis of digital images from CGH-metaphase spreads revealed a predominantly green signal on the telomeric region of chromosome 10p. After quantitative digital ratio imaging of 10 CGH-metaphase spreads, a region of gain was found in the chromosome band 10p14-pter. The CGH finding was confirmed by FISH analysis, using a whole chromosome 10 paint probe. These results show the usefulness of CGH for a rapid characterization of de novo unbalanced translocation, unidentifiable by karyotype alone.

  11. Effusive atomic oven nozzle design using an aligned microcapillary array

    Energy Technology Data Exchange (ETDEWEB)

    Senaratne, Ruwan, E-mail: rsenarat@physics.ucsb.edu; Rajagopal, Shankari V.; Geiger, Zachary A.; Fujiwara, Kurt M.; Lebedev, Vyacheslav; Weld, David M. [Department of Physics and California Institute for Quantum Emulation, University of California Santa Barbara, Santa Barbara, California 93106 (United States)

    2015-02-15

    We present a simple and inexpensive design for a multichannel effusive oven nozzle which provides improved atomic beam collimation and thus extended oven lifetimes. Using this design, we demonstrate an atomic lithium source suitable for trapped-atom experiments. At a nozzle temperature of 525 °C, the collimated atomic beam flux directly after the nozzle is 1.2 × 10{sup 14} atoms/s with a peak beam intensity greater than 5.0 × 10{sup 16} atoms/s/sr. This suggests an oven lifetime of several decades of continuous operation.

  12. Effusive Atomic Oven Nozzle Design Using a Microcapillary Array

    CERN Document Server

    Senaratne, Ruwan; Geiger, Zachary A; Fujiwara, Kurt M; Lebedev, Vyacheslav; Weld, David M

    2014-01-01

    We present a simple and inexpensive design for a multichannel effusive oven nozzle which provides improved atomic beam collimation and thus extended oven lifetimes. Using this design we demonstrate an atomic lithium source suitable for trapped-atom experiments. At a nozzle temperature of 525$^{\\circ}$C the total atomic beam flux directly after the nozzle is $1.2 \\times 10^{14}$ atoms per second with a peak beam intensity greater than $5.0 \\times 10^{16}$ atoms per second per steradian. This suggests an oven lifetime of several centuries of continuous operation.

  13. 相控阵雷达用阵面电源的结构设计%Structure Design of a Array Power Supply for Phased array Radar

    Institute of Scientific and Technical Information of China (English)

    邵奎武; 王长瑞; 肖竑

    2015-01-01

    针对某相控阵雷达用阵面电源,提出了全新设计思路,先进行概念设计,再利用ANSYS、FloEFD有限元分析软件进行力学和热仿真验证,在此基础上对阵面电源进行优化设计。结果表明,阵面电源结构减重28%,结构设计和热设计满足要求。采用此方法可有效降低设计时间及差错,实现电源的快速化、轻量化设计。%In this paper,the new design idea for array power supply of phased array radar was proposed. Firstly,conceptual structure was designed. Secondly,dynamic simulation and thermal simulation were used to verify the conceptual design results by ANSYS and FloEFD finite elements analysis software, and on this basis,optimization design of array power supply was made.The result indicates that 28% weight loss of structure for array power supply is achieved.The structure and thermal design of array power supply meet the demands.The design time and error are reduced by this method.The rapid product design and light weight design of array power supply are realized.

  14. Design of an impeller-mounted electrode array for EIT imaging

    International Nuclear Information System (INIS)

    The information content in electrical impedance tomography (EIT) reconstructions is limited by the number of independent impedance measurements acquired for each image frame. This paper describes the design and construction of an electrode array mounted onto a rotating impeller used for mixing processes. By combining the process compliant rotation of the electrodes with a dynamic measurement protocol, a substantial increase in the number of independent measurements is reported. A pitch-blade impeller is built and fitted with four electrodes. A number of challenges associated with operating a rotating electrode array have been addressed including a wireless communications protocol. The impeller has been accurately modelled and measurement simulations closely correspond to those acquired using the array. The apparatus has been used to successfully visualize a test distribution to a consistent standard compared to a conventional EIT electrode array and strategy. Presently, the limiting factor to this approach is the necessary high measurement acquistion rates which lead to restricted impeller velocities

  15. Design and Analysis of 4×4 Arrayed Waveguide Grating Multiplexer

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The operation principle of an arrayed waveguide grating(AWG) multiplexer is introduced and the 4×4 AWG with following design parameters is discussed in detail, such as the choice of wavelength, the neighboring arrayed waveguide distance ΔL, the channel frequency interval Δf, and the free spectral range. The structure of 4×4 AWG is designed and the result of stimulated test is also given. Analysis shows that the 4×4 AWG is characterized by a wide dynamic range, low crosstalk, better spectrum properties, and a compact structure.

  16. A Design of ABC95 Array Computer Multi-function Interconnection Chips

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    ABC95 array computer is a multi-function network computer based on FPGA technology. A notable feature of ABC95 array computer is the support of complex interconnection, which determines that the computer must have enough I/O band and flexible communications between Pes. The authors designed the interconnecting network chips of ABC95 and realized a form of multi-function interconnection. The multi-function interconnecting network supports conflict-free access from processors to memory matrix and the MESH network of enhanced processors to processor communications. The design scheme has been proved feasible by experiment.

  17. Experimental Tests of UltraFlex Array Designs in Low Earth Orbital and Geosynchronous Charging Environments

    Science.gov (United States)

    Galofaro, Joel T.; Vayner, Boris V.; Hillard, Grover B.

    2011-01-01

    The present ground based investigations give the first definitive look describing the expected on-orbit charging behavior of Orion UltraFlex array coupons in the Low Earth Orbital and Geosynchronous Environments. Furthermore, it is important to note that the LEO charging environment also applies to the International Space Station as well as to the lunar mission charging environments. The GEO charging environment includes the bounding case for all lunar orbital and lunar surface mission environments. The UltraFlex thin film photovoltaic array technology has been targeted to become the sole power system for life support and on-orbit power for the manned Aires Crew Exploration Vehicle. It is therefore, crucial to gain an understanding of the complex charging behavior to answer some of the basic performance and survivability issues in an attempt to ascertain that a single UltraFlex array design will be able to cope with the projected worst case LEO and GEO charging environments. Testing was limited to four array coupons, two coupons each from two different array manufactures, Emcore and Spectrolab. The layout of each array design is identical and varies only in the actual cell technology used. The individual array cells from each manufacturer have an antireflection layered coating and come in two different varieties either uncoated (only AR coating) or coated with a thin conducting ITO layer. The LEO Plasma tests revealed that all four coupons passed the arc threshold -120 V bias tests. GEO electron gun charging tests revealed that only front side area of ITO coated coupons passed tests. Only the Emcore AR array passed backside Stage 2 GEO Tests.

  18. General polynomial factorization-based design of sparse periodic linear arrays.

    Science.gov (United States)

    Mitra, Sanjit K; Mondal, Kalyan; Tchobanou, Mikhail K; Dolecek, Gordana Jovanovic

    2010-09-01

    We have developed several methods of designing sparse periodic arrays based upon the polynomial factorization method. In these methods, transmit and receive aperture polynomials are selected such that their product results in a polynomial representing the desired combined transmit/receive (T/R) effective aperture function. A desired combined T/R effective aperture is simply an aperture with an appropriate width exhibiting a spectrum that corresponds to the desired two-way radiation pattern. At least one of the two aperture functions that constitute the combined T/R effective aperture function will be a sparse polynomial. A measure of sparsity of the designed array is defined in terms of the element reduction factor. We show that elements of a linear array can be reduced with varying degrees of beam mainlobe width to sidelobe reduction properties.

  19. Design of a reconfigurable optical add/drop multiplexer based on tunable Fabry-Perot array

    Science.gov (United States)

    Ye, Jiansen; Wang, Xin; Li, Zhuo; Yang, Yang; Xu, Rui; Shi, Rui

    2015-08-01

    With the development of optical fiber communication, dense wavelength division multiplexing (DWDM) system is important for the rapid management of multi-wavelength in the core node of the optical transmission network. In this paper, a reconfigurable optical add-drop multiplexer (ROADM) based on the tunable Fabry-Perot (F-P) array is proposed. An optical switch with high isolation and low crosstalk is designed by using the characteristics of filtering and tuning for the F-P array. The principle, structure, and function of the tunable F-P array are introduced. The characteristics of filtering and tuning for the F-P filter are also calculated, and the factor for the isolation, crosstalk, response time and insertion loss are analyzed. A single physical channel ROADM with 16 signal channels, which operates in C-band, is designed and optimized by simulation.

  20. Design and analysis of an electronically steerable microstrip patch and a novel Coplanar Waveguide (CPW) fed slot antenna array

    Science.gov (United States)

    Aldossary, Hamad

    Conformal Phased Array Antennas (CPAAs) are very attractive for their high gain, low profile, and beam scanning ability while being conformal to their mounting surface. Among them are microstrip patch phased arrays and wideband slot phased arrays which are of particular significance. In this work, first the study, design, and implementation of a conformal microstrip patch phased array is presented which consists of a high gain beam scanning array implemented using microstrip delay lines controlled using GaAs SPDT switches. Then the study and design of a wideband Coplanar Waveguide (CPW)-fed slot phased array antenna is presented. In both cases the array beam scanning properties are elucidated by incorporating the measured delay line scattering parameters inside Ansys Designer simulation models and then computing and presenting their full-wave radiation characteristics.

  1. Design and Implementation of Series Micro Strip Patch Antenna Array for Wireless Communication

    Directory of Open Access Journals (Sweden)

    Priya Upadhyay

    2012-09-01

    Full Text Available Micro Strip Antenna Array has beenproposed with high efficiency for wirelesscommunication. Micro strip antenna arrays arewidely used in various applications like in wirelesscommunication system, satellite communication,Radar systems, Global positioning systems, RadioFrequency Identification (RFID, Worldwideinteroperability for microwave access (WiMax,Rectenna applications, Telemedicine applications,Medicinal applications of patch. In this articleseries micro strip square patch antenna array isdesigned due to its wide band and wide scanproperties which can give more information at highdata rate. The proposed antenna consist of astraight feeding micro strip line and squareradiating elements connected directly to the microstrip line at their corners without dividers andimpedance transformers in order to realize lowerfeeding line loss. In this paper, the model of microstrip series antenna array is designed and analyzedusing the HFSS Software. The parametric study ofthe antenna characteristics has been done to knowhow the micro strip series antenna array meets thewireless properties for geometric parameters. Thispaper micro strip series antenna array will bedesigned at 2.4 GHz (S- band frequency

  2. Bondwire array modeling for the design of hybrid high power amplifiers above C-band

    DEFF Research Database (Denmark)

    Hernández, Carlos Cilla; Jónasson, Sævar Þór; Hanberg, Jesper

    2012-01-01

    -band to simulate the large bondwire arrays that are used in long multi-transistor power bars. The usefulness of an accurate 3D EM model during the amplifier's matching network design process is highlighted using a practical example, and the effect of the insertion loss variations along the different bondwires...

  3. Design of a ring resonator-based optical beam forming network for phased array receive antennas

    NARCIS (Netherlands)

    Klooster, van 't J.W.; Roeloffzen, C.G.H.; Meijerink, A.; Zhuang, L.; Marpaung, D.A.I.; Etten, van W.C.; Heideman, R.G.; Leinse, A.; Schippers, H.; Verpoorte, J.; Wintels, M.

    2008-01-01

    A novel squint-free ring resonator-based optical beam forming network (OBFN) for phased array antennas (PAA) is proposed. It is intended to provide broadband connectivity to airborne platforms via geostationary satellites. In this paper, we present the design of the OBFN and its control system. Our

  4. Design trade-offs in ADC architectures dedicated to uncooled focal plane arrays

    Science.gov (United States)

    Robert, P.; Dupont, B.; Pochic, D.

    2008-04-01

    This paper presents two different architectures for the design of Analog to Digital Converters specifically adapted to infrared bolometric image sensors. Indeed, the increasing demand for integrated functions in uncooled readout circuits leads to on-chip ADC design as an interface between the internal analog core and the digital processing electronics. However specifying an on-chip ADC dedicated to focal plane array raises many questions about its architecture and its performance requirements. We will show that two architecture approaches are needed to cover the different sensor features in terms of array size and frame speed. A monolithic 14 bits ADC with a pipeline architecture, and a column 13 bits ADC with an original dual-ramp architecture, will be described. Finally, we will show measurement results to confirm the monolithic ADC is suitable for small array, as 160 x 120 with low frame speed, while a column ADC is more compliant for higher array, as 640 x 480 with a 60 Hz frame speed or 1024 x 768 arrays.

  5. Design of a Two-Element Antenna Array Using Substrate Integrated Waveguide Technique

    Directory of Open Access Journals (Sweden)

    Kheireddine Sellal

    2011-01-01

    Full Text Available The design of a two-element antenna array using the substrate integrated waveguide (SIW technique and operating at 10 GHz is presented. The proposed antenna array consists of two SIW phase shifter sections with two SIW slot antennas. The phase shifting is achieved by changing the position of two inductive posts inserted inside each element of the array. Numerical simulations and experimental measurements have been carried out for three differential phases between the two antenna array elements, namely, 0°, 22.5°, and 67.5°. A prototype for each differential phase has been fabricated and measured. Results have shown a fairly good agreement between theory and experiments. In fact, a reflection coefficient of better than 20 dB has been achieved around 10 GHZ. The E-plane radiation pattern has shown a beam scan between 5° and 18° and demonstrated the feasibility of designing an SIW antenna phased array.

  6. A Medium Sized Schwarzschild-Couder Cherenkov Telescope Mechanical Design Proposed for the Cherenkov Telescope Array

    CERN Document Server

    Byrum, K; Benbow, W; Cameron, R; Criswell, S; Errando, M; Guarino, V; Kaaret, P; Kieda, D; Mukherjee, R; Naumann, D; Nieto, D; Northrop, R; Okumura, A; Roache, E; Rousselle, J; Schlenstedt, S; Sternberger, R; Vassiliev, V; Wakely, S; Zhao, H

    2015-01-01

    The Cherenkov Telescope Array (CTA) is an international next-generation ground-based gamma-ray observatory. CTA will be implemented as southern and northern hemisphere arrays of tens of small, medium and large-sized imaging Cherenkov telescopes with the goal of improving the sensitivity over the current-generation experiments by an order of magnitude. CTA will provide energy coverage from ~20 GeV to more than 300 TeV. The Schwarzschild-Couder (SC) medium size (9.5m) telescopes will feature a novel aplanatic two-mirror optical design capable of accommodating a wide field-of-view with significantly improved angular resolution as compared to the traditional Davies-Cotton optical design. A full-scale prototype SC medium size telescope structure has been designed and will be constructed at the Fred Lawrence Whipple Observatory in southern Arizona during the fall of 2015. concentrate on the novel features of the design.

  7. Mechanical design of a low concentration ratio solar array for a space station application

    Science.gov (United States)

    Biss, M. S.; Hsu, L.

    1983-01-01

    This paper describes a preliminary study and conceptual design of a low concentration ratio solar array for a space station application with approximately a 100 kW power requirement. The baseline design calls for a multiple series of inverted, truncated, pyramidal optical elements with a geometric concentration ratio (GCR) of 6. It also calls for low life cycle cost, simple on-orbit maintainability, 1984 technology readiness date, and gallium arsenide (GaAs) of silicon (Si) solar cell interchangeability. Due to the large area needed to produce the amount of power required for the baseline space station, a symmetrical wing design, making maximum use of the commonality of parts approach, was taken. This paper will describe the mechanical and structural design of a mass-producible solar array that is very easy to tailor to the needs of the individual user requirement.

  8. Membrane Material-Based Rigid Solar Array Design and Thermal Simulation for Stratospheric Airships

    Directory of Open Access Journals (Sweden)

    Kangwen Sun

    2014-01-01

    Full Text Available In order to improve effective utilization of rigid solar array used in stratospheric airships here, the flexible connection design and light laminated design were introduced to rigid solar array. Based on the analysis of the design scheme, firstly, the equivalent coefficient of thermal conductivity was calculated by the theoretical formula. Subsequently, the temperature variation characteristics of the solar cell module were accurately modeled and simulated by using Computational Fluid Dynamics (CFD software. Compared to the results of test samples, the solar cell module described here guaranteed effective output as well as good heat insulating ability, effectively improving the feasibility of the stratospheric airship design. In addition, the simulation model can effectively simulate the temperature variation characteristics of the solar cell, which, therefore, provides technical support for the engineering application.

  9. Proceedings of the Flat-Plate Solar Array Project Research Forum on the design of flat-plate photovoltaic arrays for central stations

    Energy Technology Data Exchange (ETDEWEB)

    None

    1983-01-01

    The Flat-Plate Solar Array Project, managed by the Jet Propulsion Laboratory for the US Department of Energy, has focused on advancing technologies relevant to the design and construction of megawatt-level central-station systems. Photovoltaic modules and arrays for flat-plate central-station or other large-scale electric power production facilities require the establishment of a technical base that resolves design issues and results in practical and cost-effective configurations. The Central Station Research Forum addressed design, qualification and maintenance issues related to central-station arrays derived from the engineering and operating experiences of early applications and parallel laboratory research activities. Technical issues were examined from the viewpoint of the utility engineer, architect-engineer and laboratory researcher. The forum included presentations on optimum source-circuit designs, module insulation design for high system voltages, array safety, structural interface design, measurements and array operation and maintenance. The Research Forum focused on current capabilities as well as design difficulties requiring additional technological thrusts and/or continued research emphasis. Session topic summaries highlighting major points during group discussions, identifying promising technical approaches or areas of future research, are presented.

  10. Design and fabrication of polarization-insensitive hybrid solgel arrayed waveguide gratings.

    Science.gov (United States)

    Park, Soon-Ryong; Jeong, Jaewan; O, Beom-Hoan; Lee, Seung-Gol; Lee, El-Hang

    2003-03-15

    We report on the successful design and fabrication of a polarization-insensitive arrayed waveguide grating (AWG), using solgel-derived silica glass films formed on fused-silica substrates. By controlling the waveguide width and making the propagation constants of the polarizations equal, we have found it possible to fabricate polarization-insensitive solgel-based AWGs. Polarization-insensitive design improves the cross talk by approximately 10 dB in the dynamic range. PMID:12659253

  11. NASA-STD-4005 and NASA-HDBK-4006, LEO Spacecraft Solar Array Charging Design Standard

    Science.gov (United States)

    Ferguson, Dale C.

    2007-01-01

    Two new NASA Standards are now official. They are the NASA LEO Spacecraft Charging Design Standard (NASA-STD-4005) and the NASA LEO Spacecraft Charging Design Handbook (NASA-HDBK-4006). They give the background and techniques for controlling solar array-induced charging and arcing in LEO. In this paper, a brief overview of the new standards is given, along with where they can be obtained and who should be using them.

  12. Development of an Efficient Design Technique for the Optimisation of Mooring Systems for Wave Energy Arrays

    OpenAIRE

    Kirrane, P.; Fabricius, P; Morvan, R.

    2011-01-01

    Research, funded by the Marine Institute, was carried out on mooring systems for wave energy arrays. An outline of the research results and outcomes are presented in this report. The objectives of the research were to: review the wave energy industry and define design parameters; establish a comprehensive set of design curves to facilitate the selection of a preliminary mooring system; develop trends in system response from the evaluation of a broad range of Wave Energy Converter (WEC) specif...

  13. Design and fabrication of an InP arrayed waveguide grating for monolithic PICs

    Institute of Scientific and Technical Information of China (English)

    Pan Pan; An Junming; Wang Liangliang; Wu Yuanda; Wang Yue; Hu Xiongwei

    2012-01-01

    A 10-channel,200 GHz channel spacing InP arrayed waveguide grating was designed,and the deep ridge waveguide design makes it polarization independent.Under the technologies of molecular beam epitaxy,lithography,and induced coupler plasma etching,the chip was fabricated in our laboratory.The test results show that the insertion loss is about 8 dB,and the crosstalk is less than-17 dB.

  14. Monte Carlo studies of medium-size telescope designs for the Cherenkov Telescope Array

    Science.gov (United States)

    Wood, M.; Jogler, T.; Dumm, J.; Funk, S.

    2016-01-01

    We present studies for optimizing the next generation of ground-based imaging atmospheric Cherenkov telescopes (IACTs). Results focus on mid-sized telescopes (MSTs) for CTA, detecting very high energy gamma rays in the energy range from a few hundred GeV to a few tens of TeV. We describe a novel, flexible detector Monte Carlo package, FAST (FAst Simulation for imaging air cherenkov Telescopes), that we use to simulate different array and telescope designs. The simulation is somewhat simplified to allow for efficient exploration over a large telescope design parameter space. We investigate a wide range of telescope performance parameters including optical resolution, camera pixel size, and light collection area. In order to ensure a comparison of the arrays at their maximum sensitivity, we analyze the simulations with the most sensitive techniques used in the field, such as maximum likelihood template reconstruction and boosted decision trees for background rejection. Choosing telescope design parameters representative of the proposed Davies-Cotton (DC) and Schwarzchild-Couder (SC) MST designs, we compare the performance of the arrays by examining the gamma-ray angular resolution and differential point-source sensitivity. We further investigate the array performance under a wide range of conditions, determining the impact of the number of telescopes, telescope separation, night sky background, and geomagnetic field. We find a 30-40% improvement in the gamma-ray angular resolution at all energies when comparing arrays with an equal number of SC and DC telescopes, significantly enhancing point-source sensitivity in the MST energy range. We attribute the increase in point-source sensitivity to the improved optical point-spread function and smaller pixel size of the SC telescope design.

  15. Layout design studies for medium-sized telescopes within the Cherenkov Telescope Array

    CERN Document Server

    Hassan, T; Nieto, D; Wood, M

    2015-01-01

    The Cherenkov Telescope Array (CTA) is an international project for a next-generation ground-based gamma-ray observatory. CTA, conceived as an array of tens of imaging atmospheric Cherenkov telescopes, comprising small, medium and large-size telescopes, is aiming to improve on the sensitivity of current-generation experiments by an order of magnitude and provide energy coverage from 20 GeV to more than 300 TeV. In this study we explore how the medium-sized telescopes layout design and composition impacts the overall CTA performance by analyzing Monte Carlo simulations including Davies-Cotton and Schwarzschild-Couder medium-sized telescopes.

  16. Bi-Band Bow-Tie Antennas Array Design Using a Simple Equivalent Transmission Line Model

    Directory of Open Access Journals (Sweden)

    M. Abri

    2012-08-01

    Full Text Available In this paper we propose a simple equivalent and accurate transmission line model for bi-band bow-tie antennas array design over a band of frequencies for satellite communications. This model uses the resistance of a square element that appears at the edges of the antenna (radiating slots. To test this model, two antennas array were simulated and results were compared with those obtained by a rigorous method (moment’s method of the simulator ADS/Momentum. Using this transmission line approach the resonant frequency, return loss, VSWR, reflected phase, input impedance can be determined simultaneously. The results confirm the validity of the proposed model.

  17. Bi-Band Bow-Tie Antennas Array Design Using a Simple Equivalent Transmission Line Model

    OpenAIRE

    Abri, M.; H. Abri Badaoui; Dib, H; A.S.E. Gharnaout

    2012-01-01

    In this paper we propose a simple equivalent and accurate transmission line model for bi-band bow-tie antennas array design over a band of frequencies for satellite communications. This model uses the resistance of a square element that appears at the edges of the antenna (radiating slots). To test this model, two antennas array were simulated and results were compared with those obtained by a rigorous method (moment’s method) of the simulator ADS/Momentum. Using this transmission line appro...

  18. Design and synthesis of an array of selective androgen receptor modulators.

    Science.gov (United States)

    Trump, Ryan P; Blanc, Jean-Baptiste E; Stewart, Eugene L; Brown, Peter J; Caivano, Matilde; Gray, David W; Hoekstra, William J; Willson, Timothy M; Han, Bajin; Turnbull, Philip

    2007-01-01

    We describe the design, using shape comparison and fast docking computer algorithms, and rapid parallel synthesis of a 1300 member array based on GSK7721, a 4-aminobenzonitrile androgen receptor (AR) antagonist identified by focused screening of the GSK compound collection. The array yielded 352 submicromolar and 17 subnanomolar AR agonists as measured by a cell-based reporter gene functional assay. The rapid synthesis of a large number of active compounds provided valuable information in the optimization of AR modulators, which may be useful in treating androgen deficiency in aging males.

  19. Conformal Patch Antenna Arrays Design for Onboard Ship Deployment Using Genetic Algorithms

    Directory of Open Access Journals (Sweden)

    Stelios A. Mitilineos

    2013-01-01

    Full Text Available Conformal antennas and antenna arrays (arrays have become necessary for vehicular communications where a high degree of aerodynamic drag reduction is needed, like in avionics and ships. However, the necessity to conform to a predefined shape (e.g., of an aircraft’s nose directly affects antenna performance since it imposes strict constraints to the antenna array’s shape, element spacing, relative signal phase, and so forth. Thereupon, it is necessary to investigate counterintuitive and arbitrary antenna shapes in order to compensate for these constraints. Since there does not exist any available theoretical frame for designing and developing arbitrary-shape antennas in a straightforward manner, we have developed a platform combining a genetic algorithm-based design, optimization suite, and an electromagnetic simulator for designing patch antennas with a shape that is not a priori known (the genetic algorithm optimizes the shape of the patch antenna. The proposed platform is further enhanced by the ability to design and optimize antenna arrays and is intended to be used for the design of a series of antennas including conformal antennas for shipping applications. The flexibility and performance of the proposed platform are demonstrated herein via the design of a high-performance GPS patch antenna.

  20. Monte Carlo comparison of mid-size telescope designs for the Cherenkov Telescope Array

    CERN Document Server

    Jogler, T; Dumm, J

    2012-01-01

    The Cherenkov Telescope Array (CTA) is a future very high energy gamma-ray observatory. CTA will be comprised of small-, medium- and large-size telescopes covering an energy range from tens of GeV to hundreds of TeV and will surpass existing telescopes in sensitivity by an order of magnitude. The aim of our study is to find the optimal design for the medium-size telescopes (MSTs), which will determine the sensitivity in the key energy range between a few hundred GeV to about ten TeV. To study the effect of the telescope design parameters on the array performance, we simulated arrays of 61 MSTs with 120 m spacing and a variety of telescope configurations. We investigated the influence of the primary telescope characteristics including optical resolution, pixel size, and light collection area on the total array performance with a particular emphasis on telescope configurations with imaging performance similar to the proposed Davis-Cotton (DC) and Schwarzschild-Couder (SC) MST designs. We compare the performance...

  1. Monte Carlo comparison of medium-size telescope designs for the Cherenkov Telescope Array

    CERN Document Server

    Jogler, T; Dumm, J

    2013-01-01

    The Cherenkov Telescope Array (CTA) is a future very high energy gamma-ray observatory. CTA will be comprised of small-,medium- and large-size telescopes covering an energy range from tens of GeV to hundreds of TeV and will surpass existing telescopes in sensitivity by an order of magnitude. The aim of our study is to find the optimal design for the medium-size telescopes (MSTs), which will determine the sensitivity in the key energy range between a few hundred GeV to about ten TeV. To study the effect of the telescope design parameters on the array performance, we simulated arrays of 61 MSTs with 120 m spacing and a variety of telescope configurations. We investigated the influence of the primary telescope characteristics including optical resolution, pixel size, and light collection area on the total array performance with a particular emphasis on telescope configurations with imaging performance similar to the proposed Davies-Cotton (DC) and Schwarzschild-Couder (SC) MST designs. We compare the performance...

  2. Corporate-Feed Multilayer Bow-Tie Antenna Array Design Using a Simple Transmission Line Model

    Directory of Open Access Journals (Sweden)

    S. Didouh

    2012-01-01

    Full Text Available A transmission line model is used to design corporate-fed multilayered bow-tie antennas arrays; the simulated antennas arrays are designed to resonate at the frequencies 2.4 GHz, 5 GHz, and 8 GHz corresponding to RFID, WIFI, and radars applications. The contribution of this paper consists of modeling multilayer bow-tie antenna array fed through an aperture using transmission line model. The transmission line model is simple and precise and allows taking into account the whole geometrical, electrical, and technological characteristics of the antennas arrays. The proposed transmission line model showed its interest in the design of different multilayered bow-tie antennas and predicted the correct resonance frequency for different applications in telecommunications. To validate the proposed transmission line model, the simulation results obtained are compared with those obtained by the method of moments. The results of simulations are presented and discussed. Using this transmission line approach, the resonant frequency, input impedance, and return loss can be determined simultaneously. The paper reports several simulation results that confirm the validity of the developed model. The obtained results are then presented and discussed.

  3. Design of a Solar Motor Drive System Fed by a Direct-Connected Photovoltaic Array

    Directory of Open Access Journals (Sweden)

    AYDOGMUS, O.

    2012-08-01

    Full Text Available A solar motor pump drive system is modeled and simulated. The proposed drive system does not require any kind of energy storage system and dc-dc converter. The system is connected directly to a photovoltaic (PV array. Thus, a low cost solar system can be achieved. A vector controlled Permanent Magnet Synchronous Motor (PMSM is used as a solar motor to increase the efficiency of system. The motor is designed for a low rated voltage level about 24V. The hill climbing MPPT method is used for balanced the motor power and PV power to obtain a high efficiency. The results are performed by using MATLAB/SimPowerSystem blocks. In addition, the PV array is modeled to allow for the possibility of running as on-line adjustable in simulation environment without using lookup table. The performances of motor, MPPT and drive system are analyzed in different conditions as temperature and irradiation of PV array.

  4. Risk assessment models in genetics clinic for array comparative genomic hybridization: Clinical information can be used to predict the likelihood of an abnormal result in patients.

    Science.gov (United States)

    Marano, Rachel M; Mercurio, Laura; Kanter, Rebecca; Doyle, Richard; Abuelo, Dianne; Morrow, Eric M; Shur, Natasha

    2013-03-01

    Array comparative genomic hybridization (aCGH) testing can diagnose chromosomal microdeletions and duplications too small to be detected by conventional cytogenetic techniques. We need to consider which patients are more likely to receive a diagnosis from aCGH testing versus patients that have lower likelihood and may benefit from broader genome wide scanning. We retrospectively reviewed charts of a population of 200 patients, 117 boys and 83 girls, who underwent aCGH testing in Genetics Clinic at Rhode Island hospital between 1 January/2008 and 31 December 2010. Data collected included sex, age at initial clinical presentation, aCGH result, history of seizures, autism, dysmorphic features, global developmental delay/intellectual disability, hypotonia and failure to thrive. aCGH analysis revealed abnormal results in 34 (17%) and variants of unknown significance in 24 (12%). Patients with three or more clinical diagnoses had a 25.0% incidence of abnormal aCGH findings, while patients with two or fewer clinical diagnoses had a 12.5% incidence of abnormal aCGH findings. Currently, we provide families with a range of 10-30% of a diagnosis with aCGH testing. With increased clinical complexity, patients have an increased probability of having an abnormal aCGH result. With this, we can provide individualized risk estimates for each patient.

  5. Risk assessment models in genetics clinic for array comparative genomic hybridization: Clinical information can be used to predict the likelihood of an abnormal result in patients

    Science.gov (United States)

    Marano, Rachel M.; Mercurio, Laura; Kanter, Rebecca; Doyle, Richard; Abuelo, Dianne; Morrow, Eric M.; Shur, Natasha

    2013-01-01

    Array comparative genomic hybridization (aCGH) testing can diagnose chromosomal microdeletions and duplications too small to be detected by conventional cytogenetic techniques. We need to consider which patients are more likely to receive a diagnosis from aCGH testing versus patients that have lower likelihood and may benefit from broader genome wide scanning. We retrospectively reviewed charts of a population of 200 patients, 117 boys and 83 girls, who underwent aCGH testing in Genetics Clinic at Rhode Island hospital between 1 January/2008 and 31 December 2010. Data collected included sex, age at initial clinical presentation, aCGH result, history of seizures, autism, dysmorphic features, global developmental delay/intellectual disability, hypotonia and failure to thrive. aCGH analysis revealed abnormal results in 34 (17%) and variants of unknown significance in 24 (12%). Patients with three or more clinical diagnoses had a 25.0% incidence of abnormal aCGH findings, while patients with two or fewer clinical diagnoses had a 12.5% incidence of abnormal aCGH findings. Currently, we provide families with a range of 10–30% of a diagnosis with aCGH testing. With increased clinical complexity, patients have an increased probability of having an abnormal aCGH result. With this, we can provide individualized risk estimates for each patient. PMID:27625836

  6. Monte Carlo Studies of medium-size telescope designs for the Cherenkov Telescope Array

    CERN Document Server

    Wood, M; Dumm, J; Funk, S

    2015-01-01

    We present studies for optimizing the next generation of ground-based imaging atmospheric Cherenkov telescopes (IACTs). Results focus on mid-sized telescopes (MSTs) for CTA, detecting very high energy gamma rays in the energy range from a few hundred GeV to a few tens of TeV. We describe a novel, flexible detector Monte Carlo package, FAST (FAst Simulation for imaging air cherenkov Telescopes), that we use to simulate different array and telescope designs. The simulation is somewhat simplified to allow for efficient exploration over a large telescope design parameter space. We investigate a wide range of telescope performance parameters including optical resolution, camera pixel size, and light collection area. In order to ensure a comparison of the arrays at their maximum sensitivity, we analyze the simulations with the most sensitive techniques used in the field, such as maximum likelihood template reconstruction and boosted decision trees for background rejection. Choosing telescope design parameters repre...

  7. Design and Implementation of a Beam Forming Network for a Phased Array Antenna

    Directory of Open Access Journals (Sweden)

    S. Devimeena

    2015-03-01

    Full Text Available This dissertation presents a beam forming network (BFN for phased array antenna-based on coherently radiating periodic structure (CORPS. The elements of CORPS are selected in such a way to obtain broad band characteristics, good return loss and good isolation between the radiating elements. These elements were arranged in such a way that the BFN naturally produces Gaussian amplitude. This methodology reduces the complexity of the conventional phased array design making it more flexible and minimizing the loss of energy inside the structure. A phase shifter design is proposed for the CORPS. The entire BFN’s sub-blocks have been designed for the frequency band of 5.925 GHz to 6.425 GHz, which find applications in communication satellite, fixed wireless systems.Defence Science Journal, Vol. 65, No. 1, January 2015, pp.46-52, DOI:http://dx.doi.org/10.14429/dsj.65.6940

  8. Miniature Exoplanet Radial Velocity Array (MINERVA) I. Design, Commissioning, and First Science Results

    CERN Document Server

    Swift, Jonathan J; Johnson, John A; Wright, Jason T; McCrady, Nate; Wittenmyer, Robert A; Plavchan, Peter; Riddle, Reed; Muirhead, Philip S; Herzig, Erich; Myles, Justin; Blake, Cullen H; Eastman, Jason; Beatty, Thomas G; Lin, Brian; Zhao, Ming; Gardner, Paul; Falco, Emilio; Criswell, Stephen; Nava, Chantanelle; Robinson, Connor; Sliski, David H; Hedrick, Richard; Ivarsen, Kevin; Hjelstrom, Annie; de Vera, Jon; Szentgyorgyi, Andrew

    2014-01-01

    The MINiature Exoplanet Radial Velocity Array (MINERVA) is a US-based observational facility dedicated to the discovery and characterization of exoplanets around a nearby sample of bright stars. MINERVA employs a robotic array of four 0.7 m telescopes outfitted for both high-resolution spectroscopy and photometry, and is designed for completely autonomous operation. The primary science program is a dedicated radial velocity survey and the secondary science objective is to obtain high precision transit light curves. The modular design of the facility and the flexibility of our hardware allows for both science programs to be pursued simultaneously, while the robotic control software provides a robust and efficient means to carry out nightly observations. In this article, we describe the design of MINERVA including major hardware components, software, and science goals. The telescopes and photometry cameras are characterized at our test facility on the Caltech campus in Pasadena, CA, and their on-sky performance...

  9. Design and fabrication of magnetically functionalized flexible micropillar arrays for rapid and controllable microfluidic mixing

    KAUST Repository

    Zhou, BingPu

    2015-03-25

    Magnetically functionalized PDMS-based micropillar arrays have been successfully designed, fabricated and implanted for controllable microfluidic mixing. The arrangement of PDMS micropillar arrays inside the microchannel can be flexibly controlled by an external magnetic field. As a consequence, the flow fields inside the microchannel can be regulated at will via magnetic activation conveniently. When a microchannel is implanted with such micropillar arrays, two microstreams can be mixed easily and controllably upon the simple application of an on/off magnetic signal. Mixing efficiencies based on micropillar arrays with different densities were investigated and compared. It was found that micropillar arrays with higher density (i.e. smaller pillar pitch) would render better mixing performance. Our microfluidic system is capable of generating highly reproducible results within many cycles of mixing/non-mixing conversion. We believe that the simple mixing-triggering method together with rapid and controllable mixing control will be extraordinarily valuable for various biological or chemical applications in the future. This journal is © The Royal Society of Chemistry 2015.

  10. Design of 280~GHz feedhorn-coupled TES arrays for the balloon-borne polarimeter SPIDER

    CERN Document Server

    Hubmayr, Johannes; Beall, James A; Becker, Daniel T; Benton, Steven J; Bergman, A Stevie; Bond, J Richard; Bryan, Sean; Duff, Shannon M; Duivenvoorden, Adri J; Eriksen, H K; Filippini, Jeffrey P; Fraisse, Aurelien A; Galloway, Mathew; Gambrel, Anne E; Ganga, K; Grigorian, Arpi L; Gualtieri, Riccardo; Gudmundsson, Jon E; Hartley, John W; Halpern, M; Hilton, Gene C; Jones, William C; McMahon, Jeffrey J; Moncelsi, Lorenzo; Nagy, Johanna M; Netterfield, C B; Osherson, Benjamin; Padilla, Ivan; Rahlin, Alexandra S; Racine, B; Ruhl, John; Ruud, T M; Shariff, J A; Soler, J D; Song, Xue; Ullom, Joel N; Van Lanen, Jeff; Vissers, Michael R; Wehus, I K; Wen, Shyang; Wiebe, D V; Young, Edward

    2016-01-01

    We describe 280 GHz bolometric detector arrays that instrument the balloon-borne polarimeter SPIDER. A primary science goal of SPIDER is to measure the large-scale B-mode polarization of the cosmic microwave background in search of the cosmic-inflation, gravitational-wave signature. 280 GHz channels aid this science goal by constraining the level of B-mode contamination from galactic dust emission. We present the focal plane unit design, which consists of a 16$\\times$16 array of conical, corrugated feedhorns coupled to a monolithic detector array fabricated on a 150 mm diameter silicon wafer. Detector arrays are capable of polarimetric sensing via waveguide probe-coupling to a multiplexed array of transition-edge-sensor (TES) bolometers. The SPIDER receiver has three focal plane units at 280 GHz, which in total contains 765 spatial pixels and 1,530 polarization sensitive bolometers. By fabrication and measurement of single feedhorns, we demonstrate 14.7$^{\\circ}$ FHWM Gaussian-shaped beams with $<$1% ellip...

  11. Logic design for array-based circuits a structured design methodology

    CERN Document Server

    White, D E

    1992-01-01

    This book will show you how to approach the design covering everything from the circuit specification to the final design acceptance, including what support you can expect, sizing, timing analysis, power and packaging, various simulations, design verification, and design submission.

  12. CONSTRICTED PARTICLE SWARM OPTIMIZATION FOR DESIGN OF COLLINEAR ARRAY OF UNEQUAL LENGTH DIPOLE ANTENNAS

    Directory of Open Access Journals (Sweden)

    Banani Basu

    2010-06-01

    Full Text Available A method based on constricted Particle Swarm Optimization (CPSO algorithm to design a non-uniformly spaced collinear array of thin dipole antennas of unequal height is proposed. This paper presents a method for computing the appropriate excitation and geometry of individual array elements to generate a pencil beam in the vertical plane with minimum Standing Wave Ratio (SWR and fixed Side Lobe Level (SLL. Coupling effect between any two collinear center-fed thin dipole antennas having sinusoidal current distributions is analyzed using induced EMF method and minimized in terms of SWR. DRR of excitation distribution is fixed at a lower value for further mitigation of the coupling effect. Phase distribution for all the elements is kept at zero degree for broadside array. Optimization results show the effectiveness of the algorithm for the design of the array. Moreover method seems very conducive for estimating the mutual impedance between any two collinear center-fed thin dipole antennas having sinusoidal current distributions.

  13. Design of a photovoltaic central power station: flat-plate array

    Energy Technology Data Exchange (ETDEWEB)

    1984-02-01

    A design for a photovoltaic central power station using fixed flat-panel arrays has been developed. The 100 MW plant is assumed to be located adjacent to the Saguaro Power Station of Arizona Public Service. The design assumes high-efficiency photovoltaic modules using dendritic web cells. The modules are arranged in 5 MW subfields, each with its own power conditioning unit. The photovoltaic output is connected to the existing 115 kV utility switchyard. The site specific design allows detailed cost estimates for engineering, site preparation, and installation. Collector and power conditioning costs have been treated parametrically.

  14. Application of the MNA design method to a nonlinear turbofan engine. [multivariable Nyquist array method

    Science.gov (United States)

    Leininger, G. G.

    1981-01-01

    Using nonlinear digital simulation as a representative model of the dynamic operation of the QCSEE turbofan engine, a feedback control system is designed by variable frequency design techniques. Transfer functions are generated for each of five power level settings covering the range of operation from approach power to full throttle (62.5% to 100% full power). These transfer functions are then used by an interactive control system design synthesis program to provide a closed loop feedback control using the multivariable Nyquist array and extensions to multivariable Bode diagrams and Nichols charts.

  15. Design and Test Analysis of a Solar Array Root Hinge Drive Assembly

    Institute of Scientific and Technical Information of China (English)

    DING Xilun; LI Xin

    2014-01-01

    A root hinge drive assembly is preferred in place of the classical viscous damper in a large solar array system. It has advantages including better deployment control and higher reliability. But the traditional single degree of freedom model should be improved. A multiple degrees of freedom dynamics model is presented for the solar arrays deployment to guide the drive assembly design. The established model includes the functions of the torsion springs, the synchronization mechanism and the lock-up impact. A numerical computation method is proposed to solve the dynamics coupling problem. Then considering the drive torque requirement calculated by the proposed model, a root hinge drive assembly is developed based on the reliability engineering design methods, and dual actuators are used as a redundancy design. Pseudo-efficiency is introduced and the major factors influencing the (pseudo-) efficiency of the gear mechanism designed with high reduction ratio are studied for further test data analysis. A ground prototype deployment test is conducted to verify the capacity of the drive assembly. The test device consists of a large-area solar array system and a root hinge drive assembly. The RHDA development time is about 43 s. The theoretical drive torque is compared with the test values which are obtained according to the current data and the reduction efficiency analysis, and the results show that the presented model and the calibration methods are proper enough.

  16. Distinct molecular signatures in pediatric infratentorial glioblastomas defined by aCGH.

    Science.gov (United States)

    Sharma, S; Free, A; Mei, Y; Peiper, S C; Wang, Z; Cowell, J K

    2010-10-01

    Glioblastomas (GBM) are rare in children, but reportedly have more varied outcome which suggests differences in tumor etiology compared to typical GBM of adults. To investigate this we performed high resolution array comparative genomic hybridization (aCGH) analysis on three pediatric infratentorial GBM, ages 3.5, 7 and 14 years. Two of these tumors occurred in the brainstem and one in the spinal cord. While histologically typical, one brainstem tumor showed mainly pleomorphic astrocytic cells, whereas the other brainstem and spinal tumors showed a GFAP positive small cell component. Whole chromosomal gains (#1 and #2) and loss (#20) were seen only in the pleomorphic brainstem GBM, which also showed a high level of segmental genomic copy number changes. Segmental loss involving chromosome 8 was seen in all three tumors (Chr8;133039446-136869494, Chr8;pter-3581577, and Chr8;pter-30480019 respectively), whereas loss involving chromosome 16 was seen in only 2 cases with small cell components (Chr16;31827239-qter and Chr16;pter-29754532). Segmental gain of chromosome 7 was shared only between the 2 brainstem cases (Chr7;17187166-qter and Chr7;69824947-qter). Chromosome 17 showed segmental gain of 17q in the backdrop of loss of 17p only in case 1. Segmental gain of chromosome 1q was seen only in case 2. The spinal GBM showed a relatively stable karyotype with a unique loss of Chr19;32848902-qter. None of the frequent losses, gains and amplifications known to occur in adult GBM were identified, suggesting that pediatric infratentorial glioblastomas show a molecular karyotype that was more characteristic of pediatric embryonal tumors than adult GBM.

  17. DESIGN OF HYBRID COUPLER CONNECTED SQUARE ARRAY PATCH ANTENNA FOR Wi-Fi APPLICATIONS

    Directory of Open Access Journals (Sweden)

    A. Sahaya Anselin Nisha

    2012-01-01

    Full Text Available Microstrip patch antennas being popular because of light weight, low volume, thin profile configuration which can be made conformal. Wireless communication systems applications circular polarization antenna is placing vital role. In this study we introduce a new technique to produce circular polarization. Hybrid coupler is directly connected to microstrip antenna to get circular polarization. Also gain is further increased by introducing antenna array technique. Each square in array having length of 4.6mm patch is having thickness of 0.381mm and the dielectric material used FR4. The designed antenna having high gain of 6.26dB and directivity of 5.11dB at the resonant frequency of 3.7GHz. Simulation results shows that the designed antenna characteristic is suitable for Wi-Fi applications.

  18. A Design and Analysis of Dolph-Chebyshev Microstrip Planar Array Using Butler Matrix Beamforming Networks

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The design and analysis of special type beamformer, the Butler matrix, to achieve orthogonal beamforming networks is presented in this paper. A 4×4 microstrip planar array antenna is assumed to simulate a 4×4 Butler matrix to demonstrate orthogonal beamforming and beam steering. The dimensions of rectangular patches in the planar array are chosen according to the Dolph-Chebyshev current distribution in order to minimize the side-lobe level ratio for a given value of beamwidth. The simulations are carried out using an antenna design and analysis software PCAAD. It is shown that orthogonal beams can be formed to cover about 163° angle with a constant beam crossover level and high directivity.

  19. Design, Fabrication, and Packaging of an Integrated, Wirelessly-Powered Optrode Array for Optogenetics Application

    OpenAIRE

    Wen eLi; Ki Yong eKwon; Hyung-Min eLee; Maysam eGhovanloo; Arthur eWeber

    2015-01-01

    The recent development of optogenetics has created an increased demand for advancing engineering tools for optical modulation of neural circuitry. This paper details the design, fabrication, integration, and packaging procedures of a wirelessly-powered, light emitting diode (LED) coupled optrode neural interface for optogenetic studies. The LED-coupled optrode array employs microscale LED (μLED) dies and polymer-based microwaveguides to deliver light into multi-level cortical networks, coup...

  20. Design, fabrication, and packaging of an integrated, wirelessly-powered optrode array for optogenetics application

    OpenAIRE

    Kwon, Ki Yong; Lee, Hyung-Min; Ghovanloo, Maysam; Weber, Arthur; Li, Wen

    2015-01-01

    The recent development of optogenetics has created an increased demand for advancing engineering tools for optical modulation of neural circuitry. This paper details the design, fabrication, integration, and packaging procedures of a wirelessly-powered, light emitting diode (LED) coupled optrode neural interface for optogenetic studies. The LED-coupled optrode array employs microscale LED (μLED) chips and polymer-based microwaveguides to deliver light into multi-level cortical networks, coupl...

  1. Design, modeling and testing of the Askaryan Radio Array South Pole autonomous renewable power stations

    Energy Technology Data Exchange (ETDEWEB)

    Besson, D.Z., E-mail: zedlam@ku.edu [Department of Physics and Astronomy, University of Kansas, 1082 Malott Hall, 1251 Wescoe Hall Drive, Lawrence, KS 66045-7582 (United States); Moscow Engineering and Physics Institute, 31 Kashirskaya Highway, Moscow 115409 (Russian Federation); Kennedy, D.M., E-mail: dmkennedy@ku.edu [Department of Physics and Astronomy, University of Kansas, 1082 Malott Hall, 1251 Wescoe Hall Drive, Lawrence, KS 66045-7582 (United States); Ratzlaff, K., E-mail: ratzlaff@ku.edu [Instrumentation Design Laboratory, University of Kansas, 6042 Malott Hall, 1251 Wescoe Hall Drive, Lawrence, KS 66045-7582 (United States); Young, R., E-mail: rwyoung@ku.edu [Instrumentation Design Laboratory, University of Kansas, 6042 Malott Hall, 1251 Wescoe Hall Drive, Lawrence, KS 66045-7582 (United States)

    2014-11-01

    We describe the design, construction and operation of the Askaryan Radio Array (ARA) Autonomous Renewable Power Stations, initially installed at the South Pole in December, 2010 with the goal of providing an independently operating 100 W power source capable of year-round operation in extreme environments. In addition to particle astrophysics applications at the South Pole, such a station can easily be, and has since been, extended to operation elsewhere, as described herein.

  2. Microlens array-based high-gain screen design for direct projection head-up displays

    OpenAIRE

    Hedili, M. Kıvanç; Ürey, Hakan; Freeman, Mark O.

    2013-01-01

    Microlens array-based high-gain screen design for direct projection head-up displays M. Kivanc Hedili,1 Mark O. Freeman,2 and Hakan Urey1,* 1Optical Microsystems Laboratory, Koc University, Rumelifeneri Yolu, Sarıyer, İstanbul 34450, Turkey 2Lost Lake Technology LLC, 21623 W. Lost Lake Rd., Snohomish, Washington 98296, USA *Corresponding author: Received 2 October 2012; revised 28 December 2012; accepted 29 December 2012; posted 18 January 2013 (Doc. ID 17...

  3. Design and fabrication of microlens and spatial filter array by self-alignment

    Science.gov (United States)

    Yang, Ren; Chan, Kin Foong; Feng, Zhiqiang; Mei, Wenhui

    2003-01-01

    For typically small volume production of MEMS, MOEMS, fine feature PCB, high density chip packaging and display panels, especially for lab tests, low cost and the capability to change the original design easily and quickly are very important for customers and researchers. BALL Semiconductor Inc.'s Maskless Lithography Systems (MLS) feature the Digital Mirror Device (DMD) as the pattern generator to replace photo-masks. This can remove masks from UV lithography, and dramatically reduce the running cost and save time for lab tests and small volume production. At Ball Semiconductor Inc, 1.5μm line/space, 10μm line/space, and 20μm line/space Maskless Lithography Systems were developed. In our MLS, an 848×600 microlens and spatial filter array (MLSFA) was used to focus the light and to filter the noise. In order to produce smaller line-space than 16μm the MLSFA was used to get smaller UV light pad (compared with the SVGA DMD"s micro-mirror: 17μm×17μm) and to filter the noise produced from the DMD, optical lens system, and micro lens array. This MLSFA is one of the key devices for our Maskless Lithography System, and determines the resolution and quality of maskless lithography. A novel design and fabrication process of a single-package MLSFA for our Maskless Lithography System will be introduced. To avoid problems produced by misalignment between a two-piece spatial filter and microlens array, MEMS processing is used to integrate the microlens array with the spatial filter array. In this paper, the self-alignment method used to fabricate exactly matched MLSFA will be presented.

  4. Analysis and design of coupled-oscillator arrays for microwave systems

    Science.gov (United States)

    Moussounda, Renaud

    The concept of synchronized nonlinear coupled oscillators is applied to microwave and antenna engineering for the analysis and design of wireless communication and sensing systems operating at the microwave and/or millimeter (mm)-wave frequencies. The significance of such approach is justified from the potential gain in efficiency, weight, cost and functionality although technical challenges stand in the way. Unlike typical phased array systems, which are currently used to construct such systems, coupled-oscillator systems present additional challenges that mainly arise from maintaining stability and synchronization as the the coupled nonlinear system is operated. Linear systems do not present such stability issues and are consequently faster since they do not rely on any gradual synchronization mechanism in order to function. However, at significantly higher frequencies in the quasi-optical domain, coupled-oscillator systems can make up for the speed difference and present significant efficiency advantages over typical phased array architectures. In addition, coupled nonlinear systems possess inherent analog properties that can be used for a multitude of functions. This dissertation advances the topic of coupled-oscillator arrays by 1) developing an alternative set of techniques for designing the oscillating unit cells called active integrated antennas (AIAs) at microwave or mm-wave frequencies, 2) developing a more accurate description of the dynamics of the array, 3) developing and implementing a new topology for a coupling network that is able to extend stability, 4) implementing a fully non-reciprocally coupled array able to produce large scan angle without loss of stability, 5) proposing an architecture based on a single phase-locked loop (PLL) and containing a self-calibration mechanism, and finally 6) implementing a phase-boosting mechanism using simple circuits to amplify the phase difference between adjacent radiating antennas in order to increase

  5. MR thermometry characterization of a hyperthermia ultrasound array designed using the k-space computational method

    Directory of Open Access Journals (Sweden)

    Lee Hotaik

    2006-10-01

    Full Text Available Abstract Background Ultrasound induced hyperthermia is a useful adjuvant to radiation therapy in the treatment of prostate cancer. A uniform thermal dose (43°C for 30 minutes is required within the targeted cancerous volume for effective therapy. This requires specific ultrasound phased array design and appropriate thermometry method. Inhomogeneous, acoustical, three-dimensional (3D prostate models and economical computational methods provide necessary tools to predict the appropriate shape of hyperthermia phased arrays for better focusing. This research utilizes the k-space computational method and a 3D human prostate model to design an intracavitary ultrasound probe for hyperthermia treatment of prostate cancer. Evaluation of the probe includes ex vivo and in vivo controlled hyperthermia experiments using the noninvasive magnetic resonance imaging (MRI thermometry. Methods A 3D acoustical prostate model was created using photographic data from the Visible Human Project®. The k-space computational method was used on this coarse grid and inhomogeneous tissue model to simulate the steady state pressure wavefield of the designed phased array using the linear acoustic wave equation. To ensure the uniformity and spread of the pressure in the length of the array, and the focusing capability in the width of the array, the equally-sized elements of the 4 × 20 elements phased array were 1 × 14 mm. A probe was constructed according to the design in simulation using lead zerconate titanate (PZT-8 ceramic and a Delrin® plastic housing. Noninvasive MRI thermometry and a switching feedback controller were used to accomplish ex vivo and in vivo hyperthermia evaluations of the probe. Results Both exposimetry and k-space simulation results demonstrated acceptable agreement within 9%. With a desired temperature plateau of 43.0°C, ex vivo and in vivo controlled hyperthermia experiments showed that the MRI temperature at the steady state was 42.9 ± 0.38

  6. Design of Circle Array Pattern for Transparent Nanomesh-Type Electrodes.

    Science.gov (United States)

    Shin, Seungsu; Ganorkar, Shraddha; Kim, Jungyoon; Kim, Young-Hwan; Kim, Yong Tae; Kim, Seong-Il

    2015-10-01

    In this study, we report simulation results for the optical properties of nanomesh-type electrodes for transparent electrode. Usually, indium tin oxide (ITO) is commonly used for transparent conductor to solar cell, display, LED and other electronic modules. However, the cost is high due to the rarity of indium. An alternative way is use of transparent nanomesh-type metal electrode for solar cell. Finite different time domain (FDTD) method was used to simulate and analyze their optical properties. Various array patterns (square, circle and circle hexagonal array) of metal on glass (SiO2) substrates were designed and their properties of transmission and reflection were simulated by using the broad band (λ = 300~1200 nm) plane wave source. To compare basic characteristics of each pattern, various structural parameters such as hole diameter, pitch and metal thickness were applied. The effect of three typical metals, such as Ag, Au, and Al was also compared. In the results, we will discuss about large dip of transmission appeared at the particular wavelength region from the circle array pattern, which is design with pitch of 500 nm at the wavelength range 700 to 800 nm. The surface plasmonpolaritons (SPP) effect can be the cause of large dip and we could find a broad and high transmission from the properly designed in nanomesh-type electrodes, which was calculated as a function of wavelength. PMID:26726388

  7. Design of Thin-Film-Transistor (TFT) arrays using current mirror circuits for Flat Panel Detectors (FPDs)

    OpenAIRE

    Salahuddin, Nur Sultan; Paindavoine, Michel; Huda, Nurul; Parmentier, Michel

    2011-01-01

    We designed 4x4 matrix TFTs arrays using current mirror amplifiers. Advantages of current mirror amplifiers are they need less requiring switches and the conversion time is short. The TFTs arrays 4x4 matrix using current mirror circuits have been fabricated and tested with success. The TFTs array directly can process signals coming from 16 pixels in the same node. This enables us to make the summation of the light intensities of close pixels during a reading.

  8. [Confirmation of a prenatal diagnosis of trisomy 13 with comparative genomic hybridization (CGH)].

    Science.gov (United States)

    Marton, T; Thein, A; Bán, Z; Soothill, P; Oroszné, N J; Papp, Z

    2001-05-13

    Trisomy 13 was diagnosed with genetic amniocentesis in a fetus of a 50 years old patient. Fetopathologic examination has shown cyclopy, proboscis and semilobar holoprosencephaly of the fetus, which is consistent with Patau syndrome. DNA was extracted from frozen liver tissue. Result of comparative genomic hybridization (CGH) was consistent with trisomy 13. They processed the DNA according Kallioniemi's method with modifications. CGH was developed for cancer genetics in mid 90s and now it is widely used in prenatal diagnosis too. CGH allows global analysis to detect unbalanced chromosome gains and losses in the whole genome in a single experiment without the need for cell culture. Significant results can be expected in those cases where conventional cytogenetics is not able to provide an answer either because postmortem tissue is not appropriate for cytogenetics or because the chromosomal change is sub-microscopical. CGH is a fluorescent in situ hybridization on a healthy target metaphase, with equal amount of competitive hybridization of green labelled digested test DNA and red labelled digested control DNA. Red to green ratio is assessed with the help of an image analyser. Green dominance represents chromosome gain, while red shift chromosome loss. In the paper they present the fetopathologic report of a trisomy 13 fetus and illustrate the method being the first Hungarian obstetric case diagnosed by CGH. PMID:11419300

  9. Innovative enclosure dome/observing aperture system design for the MROI Array Telescopes

    Science.gov (United States)

    Busatta, A.; Marchiori, G.; Mian, S.; Payne, I.; Pozzobon, M.

    2010-07-01

    The close-pack array of the MROI necessitated an original design for the Unit Telescope Enclosure (UTE) at Magdalena Ridge Observatory. The Magdalena Ridge Observatory Interferometer (MROI) is a project which comprises an array of up to ten (10) 1.4m diameter mirror telescopes arranged in a "Y" configuration. Each of these telescopes will be housed inside a Unit Telescope Enclosure (UTE) which are relocatable onto any of 28 stations. The most compact configuration includes all ten telescopes, several of which are at a relative distance of less than 8m center to center from each other. Since the minimum angle of the field of regard is 30° with respect to the horizon, it is difficult to prevent optical blockage caused by adjacent UTEs in this compact array. This paper presents the design constraints inherent in meeting the requirement for the close-pack array. An innovative design enclosure was created which incorporates an unique dome/observing aperture system. The description of this system focuses on how the field of regard requirement led to an unique and highly innovative concept that had to be able to operate in the harsh environmental conditions encountered at an altitude of 10,460ft (3,188m). Finally, we describe the wide use of composites materials and structures (e.g. glass/carbon fibres, sandwich panels etc.) on the aperture system which represents the only way to guarantee adequate thermal and environmental protection, compactness, structural stability and limited power consumption due to reduced mass.

  10. Design, microfabrication, and analysis of micrometer-sized cylindrical ion trap arrays

    Science.gov (United States)

    Cruz, D.; Chang, J. P.; Fico, M.; Guymon, A. J.; Austin, D. E.; Blain, M. G.

    2007-01-01

    A description of the design and microfabrication of arrays of micrometer-scale cylindrical ion traps is offered. Electrical characterization and initial ion trapping experiments with a massively parallel array of 5μm internal radius (r0) sized cylindrical ion traps (CITs) are also described. The ion trap, materials, and design are presented and shown to be critical in achieving minimal trapping potential while maintaining minimal power consumption. The ion traps, fabricated with metal electrodes, have inner radii of 1, 2, 5, and 10μm and range from 5to24μm in height. The electrical characteristics of packaged ion trap arrays were measured with a vector network analyzer. The testing focused on trapping toluene (C7H8), mass 91, 92, or 93amu, in the 5μm sized CITs. Ions were formed via electron impact ionization and were ejected by turning off the rf voltage applied to the ring electrode; a current signal was collected at this time. Optimum ionization and trapping conditions, such as a sufficient pseudopotential well and high ionization to ion loss rate ratio (as determined by simulation), proved to be difficult to establish due to the high device capacitance and the presence of exposed dielectric material in the trapping region. However, evidence was obtained suggesting the trapping of ions in 1%-15% of the traps in the array. These first tests on micrometer-scale CITs indicated the necessary materials and device design modifications for realizing ultrasmall and low power ion traps.

  11. Analysis and design of box-like filters based on 3×2 microring resonator arrays

    Science.gov (United States)

    Zhang, Xiaobei; Zhang, Xinliang; Huang, Dexiu

    2009-11-01

    This paper theoretically investigates spectral characteristics of the 3×2 microring resonator array, with its analytical model developed firstly. Simulation results show that the case of the ring-bus coupling coefficient smaller than the ringring coupling coefficient is suitable for box-like filters. After design principles are given, the optimization process are carried out by evaluating the side lobe rejection ratio, the passband ripple rejection ratio and the roll-off coefficient of the passband edge. The FWHM of the designed box-like filter can be adjustable in a flexible range.

  12. Design and array signal suggestion of array type pulsed eddy current probe for health monitoring of metal tubes

    Energy Technology Data Exchange (ETDEWEB)

    Shin, Young Kil [Dept. of Electrical Engineering, Kunsan National University, Kunsan (Korea, Republic of)

    2015-10-15

    An array type probe for monitoring metal tubes is proposed in this paper which utilizes peak value and peak time of a pulsed eddy current(PEC) signal. The probe consists of an array of encircling coils along a tube and the outside of coils is shielded by ferrite to prevent source magnetic fields from directly affecting sensor signals since it is the magnetic fields produced by eddy currents that reflect the condition of metal tubes. The positions of both exciter and sensor coils are consecutively moved automatically so that manual scanning is not necessary. At one position of send-receive coils, peak value and peak time are extracted from a sensor PEC signal and these data are accumulated for all positions to form an array type peak value signal and an array type peak time signal. Numerical simulation was performed using the backward difference method in time and the finite element method for spatial analysis. Simulation results showed that peak value increases and the peak appears earlier as the defect depth or length increases. The proposed array signals are shown to be excellent in reflecting the defect location as well as variations of defect depth and length within the array probe.

  13. Direct Closed-Form Design of Finite Alphabet Constant Envelope Waveforms for Planar Array Beampatterns

    KAUST Repository

    Bouchoucha, Taha

    2015-05-01

    Multiple Input Multiple Output (MIMO) radar systems has attracted lately a lot of attention thanks to its advantage over the classical phased array radar systems. We site among these advantages the improvement of parametric identifiability, achievement of higher spatial resolution and design of complex beampatterns. In colocated multiple-input multiple-output radar systems, it is usually desirable to steer transmitted power in the region-of-interest in order to increase the Signal to Noise Ratio (SNR) and reduce any undesired signal and thus improve the detection process. This problem is also known as transmit beampattern design. To achieve this goal, conventional methods optimize the waveform covariance matrix, R, for the desired beampattern, which is then used to generate the actual transmitted waveforms. Both steps require constrained optimization. Most of the existing methods use iterative algorithms to solve these problems, therefore their computational complexity is very high which makes them hard to use in practice especially for real time radar applications. In this paper, we provide a closed-form solution to design the covariance matrix for a given beampattern in the three dimensional space using planar arrays, which is then used to derive a novel closed-form algorithm to directly design the finite-alphabet constant-envelope waveforms. The proposed algorithm exploits the two-dimensional discrete Fourier transform which is implemented using fast Fourier transform algorithm. Consequently, the computational complexity of the proposed beampattern solution is very low allowing it to be used for large arrays to change the beampattern in real time. We also show that the number of required snapshots in each waveform depends on the beampattern and that it is less than the total number of transmit antennas. In addition, we show that the proposed waveform design method can be used with non symmetric beampatterns. The performance of our proposed algorithm compares

  14. Detection of chromosome imbalances in retinoblastoma by parallel karyotype and CGH analyses.

    Science.gov (United States)

    Mairal, A; Pinglier, E; Gilbert, E; Peter, M; Validire, P; Desjardins, L; Doz, F; Aurias, A; Couturier, J

    2000-08-01

    We have studied a series of 20 primary retinoblastomas by karyotypic analysis and comparative genomic hybridization (CGH), to perform an exhaustive evaluation of chromosome imbalances in this tumor. In addition, 4 tumors were studied by CGH only. On the whole, CGH results were largely in agreement with those of karyotypic analysis and with known cytogenetic data. The most frequent imbalances were +6p (13/24 cases), +1q (12/24), -16/-16q (11/24), and +2p (9/24). Recurrent high-level amplifications were observed in 2p23-25 and 1q21. Amplification of 2p23-25, present in 4 cases among which 3 showed double-minute chromosomes, was related to MYCN amplification, as demonstrated by FISH and PCR. No evident correlation was found in this small series between any of the imbalances identified and either the differentiation or the histoprognostic risk. PMID:10862045

  15. Accurate compressed look up table method for CGH in 3D holographic display.

    Science.gov (United States)

    Gao, Chuan; Liu, Juan; Li, Xin; Xue, Gaolei; Jia, Jia; Wang, Yongtian

    2015-12-28

    Computer generated hologram (CGH) should be obtained with high accuracy and high speed in 3D holographic display, and most researches focus on the high speed. In this paper, a simple and effective computation method for CGH is proposed based on Fresnel diffraction theory and look up table. Numerical simulations and optical experiments are performed to demonstrate its feasibility. The proposed method can obtain more accurate reconstructed images with lower memory usage compared with split look up table method and compressed look up table method without sacrificing the computational speed in holograms generation, so it is called accurate compressed look up table method (AC-LUT). It is believed that AC-LUT method is an effective method to calculate the CGH of 3D objects for real-time 3D holographic display where the huge information data is required, and it could provide fast and accurate digital transmission in various dynamic optical fields in the future. PMID:26831987

  16. Visual Basic语言数组程序的设计%Design of Array Program for Visual Basic Language Programming

    Institute of Scientific and Technical Information of China (English)

    魏邦龙; 岳秋菊; 冯婕; 魏赟; 海波

    2015-01-01

    本文介绍了Visual Basic语言的两种数组,提出了正确理解和设计不同数组的方法和步骤,并通过实例对静态数组和动态数组的定义进行了讨论。%This paper introduced two kinds of arrays for Visual Basic language Programming and put forward the methods and procedures to correctly understand and design different arrays, and then discussed the definitions of static array and dynamic array by an example.

  17. 2D Active Antenna Array Design for FD-MIMO System and Antenna Virtualization Techniques

    Directory of Open Access Journals (Sweden)

    Ioannis Tzanidis

    2015-01-01

    Full Text Available Full dimension MIMO (FD-MIMO is one of the key technologies presently studied in the 3GPP for the next generation long-term evolution advanced (LTE-A systems. By incorporating FD-MIMO into LTE/LTE-A systems, it is expected that system throughput will be drastically improved beyond what is possible in conventional LTE systems. This paper presets details on the 2D active antenna array design for FD-MIMO systems supporting 32 antenna elements. The FD-MIMO system allows for dynamic and adaptive precoding to be performed jointly across all antennas thus achieving more directional transmissions in the azimuth and elevation domains simultaneously, to a larger number of users. Finally, we discuss 2D antenna array port virtualization techniques for creating beams with wide coverage, necessary for broadcasting signals to all users within a sector, such as the CRS (Common Reference Signal.

  18. Autoantibody profiling of benign and malignant thyroid tumors and design of a prototype diagnostic array

    Directory of Open Access Journals (Sweden)

    K V Lanshchakov

    2012-06-01

    Full Text Available Currently the “gold standard” in diagnostics of thyroid tumors is a fine-needle aspiration cytology (FNAC. However, FNAC cannot discriminate between benign and malignant thyroid tumors in 15 to 30% of observations. In order to develop an additional tool for differential diagnostics of thyroid tumors we evaluated the diagnostic performance of 3-antigen serum autoantibody signature in groups of benign ( n = 22 and malignant ( n = 26 thyroid tumors using a dot-blot ELISA-based analysis The sensitivity and specificity of resultant array were estimated to be 55–60% and 95–100%, respectively ( p < 0.001 according to one-sided Fisher Exact Test. Thus, we created a prototype antigen array for differential diagnostics of thyroid tumors which can be regarded as a platform for design of more complicated panel, highly sensitive in thyroid cancer detection, which can significantly improve the accuracy of preoperative diagnosis of thyroid cancer.

  19. DSTN (Distributed Sleep Transistor Network) for Low Power Programmable Logic array Design

    CERN Document Server

    Singla, Pradeep; Malik, Naveen Kr; 10.5120/7004-9563

    2012-01-01

    With the high demand of the portable electronic products, Low- power design of VLSI circuits & Power dissipation has been recognized as a challenging technology in the recent years. PLA (Programming logic array) is one of the important off shelf part in the industrial application. This paper describes the new design of PLA using power gating structure sleep transistor at circuit level implementation for the low power applications. The important part of the power gating design i.e. header and footer switch selection is also describes in the paper. The simulating results of the proposed architecture of the new PLA is shown and compared with the conventional PLA. This paper clearly shows the optimization in the reduction of power dissipation in the new design implementation of the PLA. The transient response of the power gates structure of PLA is also illustrate in the paper by using TINA-PRO software.

  20. Miniature objective lens for array digital pathology: design improvement based on clinical evaluation

    Science.gov (United States)

    McCall, Brian; Pierce, Mark; Graviss, Edward A.; Richards-Kortum, Rebecca R.; Tkaczyk, Tomasz S.

    2016-03-01

    A miniature objective designed for digital detection of Mycobacterium tuberculosis (MTB) was evaluated for diagnostic accuracy. The objective was designed for array microscopy, but fabricated and evaluated at this stage of development as a single objective. The counts and diagnoses of patient samples were directly compared for digital detection and standard microscopy. The results were found to be correlated and highly concordant. The evaluation of this lens by direct comparison to standard fluorescence sputum smear microscopy presented unique challenges and led to some new insights in the role played by the system parameters of the microscope. The design parameters and how they were developed are reviewed in light of these results. New system parameters are proposed with the goal of easing the challenges of evaluating the miniature objective and maintaining the optical performance that produced the agreeable results presented without over-optimizing. A new design is presented that meets and exceeds these criteria.

  1. Design and optimization of arrays of neodymium iron boron-based magnets for magnetic tweezers applications.

    Science.gov (United States)

    Zacchia, Nicholas A; Valentine, Megan T

    2015-05-01

    We present the design methodology for arrays of neodymium iron boron (NdFeB)-based magnets for use in magnetic tweezers devices. Using finite element analysis (FEA), we optimized the geometry of the NdFeB magnet as well as the geometry of iron yokes designed to focus the magnetic fields toward the sample plane. Together, the magnets and yokes form a magnetic array which is the basis of the magnetic tweezers device. By systematically varying 15 distinct shape parameters, we determined those features that maximize the magnitude of the magnetic field gradient as well as the length scale over which the magnetic force operates. Additionally, we demonstrated that magnetic saturation of the yoke material leads to intrinsic limitations in any geometric design. Using this approach, we generated a compact and light-weight magnetic tweezers device that produces a high field gradient at the image plane in order to apply large forces to magnetic beads. We then fabricated the optimized yoke and validated the FEA by experimentally mapping the magnetic field of the device. The optimization data and iterative FEA approach outlined here will enable the streamlined design and construction of specialized instrumentation for force-sensitive microscopy. PMID:26026529

  2. Design and optimization of arrays of neodymium iron boron-based magnets for magnetic tweezers applications

    International Nuclear Information System (INIS)

    We present the design methodology for arrays of neodymium iron boron (NdFeB)-based magnets for use in magnetic tweezers devices. Using finite element analysis (FEA), we optimized the geometry of the NdFeB magnet as well as the geometry of iron yokes designed to focus the magnetic fields toward the sample plane. Together, the magnets and yokes form a magnetic array which is the basis of the magnetic tweezers device. By systematically varying 15 distinct shape parameters, we determined those features that maximize the magnitude of the magnetic field gradient as well as the length scale over which the magnetic force operates. Additionally, we demonstrated that magnetic saturation of the yoke material leads to intrinsic limitations in any geometric design. Using this approach, we generated a compact and light-weight magnetic tweezers device that produces a high field gradient at the image plane in order to apply large forces to magnetic beads. We then fabricated the optimized yoke and validated the FEA by experimentally mapping the magnetic field of the device. The optimization data and iterative FEA approach outlined here will enable the streamlined design and construction of specialized instrumentation for force-sensitive microscopy

  3. Miniature Exoplanet Radial Velocity Array (MINERVA) I. Design, Commissioning, and First Science Results

    Science.gov (United States)

    Swift, Jonathan J.; Bottom, Michael; Johnson, John A.; Wright, Jason T.; McCrady, Nate; Wittenmyer, Robert A.; Plavchan, Peter; Riddle, Reed; Muirhead, Philip S.; Herzig, Erich; Myles, Justin; Blake, Cullen H.; Eastman, Jason; Beatty, Thomas G.; Barnes, Stuart I.; Gibson, Steven R.; Lin, Brian; Zhao, Ming; Gardner, Paul; Falco, Emilio; Criswell, Stephen; Nava, Chantanelle; Robinson, Connor; Sliski, David H.; Hedrick, Richard; Ivarsen, Kevin; Hjelstrom, Annie; de Vera, Jon; Szentgyorgyi, Andrew

    2015-04-01

    The Miniature Exoplanet Radial Velocity Array (MINERVA) is a U.S.-based observational facility dedicated to the discovery and characterization of exoplanets around a nearby sample of bright stars. MINERVA employs a robotic array of four 0.7-m telescopes outfitted for both high-resolution spectroscopy and photometry, and is designed for completely autonomous operation. The primary science program is a dedicated radial velocity survey and the secondary science objective is to obtain high-precision transit light curves. The modular design of the facility and the flexibility of our hardware allows for both science programs to be pursued simultaneously, while the robotic control software provides a robust and efficient means to carry out nightly observations. We describe the design of MINERVA, including major hardware components, software, and science goals. The telescopes and photometry cameras are characterized at our test facility on the Caltech campus in Pasadena, California, and their on-sky performance is validated. The design and simulated performance of the spectrograph is briefly discussed as we await its completion. New observations from our test facility demonstrate sub-mmag photometric precision of one of our radial velocity survey targets, and we present new transit observations and fits of WASP-52b-a known hot-Jupiter with an inflated radius and misaligned orbit. The process of relocating the MINERVA hardware to its final destination at the Fred Lawrence Whipple Observatory in southern Arizona has begun, and science operations are expected to commence in 2015.

  4. 22q13.3 Deletion Syndrome : Clinical and Molecular Analysis Using Array CGH

    NARCIS (Netherlands)

    Dhar, S. U.; del Gaudio, D.; German, J. R.; Peters, S. U.; Ou, Z.; Bader, P. I.; Berg, J. S.; Blazo, M.; Brown, C. W.; Graham, B. H.; Grebe, T. A.; Lalani, S.; Irons, M.; Sparagana, S.; Williams, M.; Phillips, J. A.; Beaudet, A. L.; Stankiewicz, P.; Patel, A.; Cheung, S. W.; Sahoo, T.

    2010-01-01

    The 22q13.3 deletion syndrome results from loss of terminal segments of varying sizes at 22qter. Few genotype phenotype correlations have been found but all patients have mental retardation and severe delay, or absence of, expressive speech. We carried out clinical and molecular characterization of

  5. Matching of array CGH and gene expression microarray features for the purpose of integrative genomic analyses

    Directory of Open Access Journals (Sweden)

    van Wieringen Wessel N

    2012-05-01

    Full Text Available Abstract Background An increasing number of genomic studies interrogating more than one molecular level is published. Bioinformatics follows biological practice, and recent years have seen a surge in methodology for the integrative analysis of genomic data. Often such analyses require knowledge of which elements of one platform link to those of another. Although important, many integrative analyses do not or insufficiently detail the matching of the platforms. Results We describe, illustrate and discuss six matching procedures. They are implemented in the R-package sigaR (available from Bioconductor. The principles underlying the presented matching procedures are generic, and can be combined to form new matching approaches or be applied to the matching of other platforms. Illustration of the matching procedures on a variety of data sets reveals how the procedures differ in the use of the available data, and may even lead to different results for individual genes. Conclusions Matching of data from multiple genomics platforms is an important preprocessing step for many integrative bioinformatic analysis, for which we present six generic procedures, both old and new. They have been implemented in the R-package sigaR, available from Bioconductor.

  6. Prenatal detection and characterization of supernumerary marker chromosomes by array-CGH

    Science.gov (United States)

    Small supernumerary marker chromosomes (sSMC) occur in about 0.043% of newborns and in 0.076% of prenatal diagnoses. The phenotypes associated with sSMC vary substantially depending on size, gene content, and chromosome origin, which cannot easily be determined by karyotype or FISH analysis. There...

  7. Assessment of genome integrity in cattle transgenic cell lines using array CGH

    Science.gov (United States)

    Transgenic cattle carrying multiple genomic modifications have been produced by serial rounds of somatic cell chromatin transfer (cloning) of sequentially genetically targeted somatic cells. However, cloning efficiency tends to decline with the increase of rounds of cloning. It is possible that mult...

  8. Design of coated standing nanowire array solar cell performing beyond the planar efficiency limits

    Science.gov (United States)

    Zeng, Yang; Ye, Qinghao; Shen, Wenzhong

    2016-05-01

    The single standing nanowire (SNW) solar cells have been proven to perform beyond the planar efficiency limits in both open-circuit voltage and internal quantum efficiency due to the built-in concentration and the shifting of the absorption front. However, the expandability of these nano-scale units to a macro-scale photovoltaic device remains unsolved. The main difficulty lies in the simultaneous preservation of an effective built-in concentration in each unit cell and a broadband high absorption capability of their array. Here, we have provided a detailed theoretical guideline for realizing a macro-scale solar cell that performs furthest beyond the planar limits. The key lies in a complementary design between the light-trapping of the single SNWs and that of the photonic crystal slab formed by the array. By tuning the hybrid HE modes of the SNWs through the thickness of a coaxial dielectric coating, the optimized coated SNW array can sustain an absorption rate over 97.5% for a period as large as 425 nm, which, together with the inherited carrier extraction advantage, leads to a cell efficiency increment of 30% over the planar limit. This work has demonstrated the viability of a large-size solar cell that performs beyond the planar limits.

  9. Closed-form Solution to Directly Design FACE Waveforms for Beampatterns Using Planar Array

    KAUST Repository

    Bouchoucha, Taha

    2015-04-19

    In multiple-input multiple-output radar systems, it is usually desirable to steer transmitted power in the region-of-interest. To do this, conventional methods optimize the waveform covariance matrix, R, for the desired beampattern, which is then used to generate actual transmitted waveforms. Both steps require constrained optimization, therefore, use iterative algorithms. The main challenges encountered in the existing approaches are the computational complexity and the design of waveforms to use in practice. In this paper, we provide a closed-form solution to design covariance matrix for the given beampattern using the planar array, which is then used to derive a novel closed-form algorithm to directly design the finite-alphabet constant-envelope (FACE) waveforms. The proposed algorithm exploits the two-dimensional fast-Fourier-transform. The performance of our proposed algorithm is compared with existing methods that are based on semi-definite quadratic programming with the advantage of a considerably reduced complexity.

  10. VLSI design of an RSA encryption/decryption chip using systolic array based architecture

    Science.gov (United States)

    Sun, Chi-Chia; Lin, Bor-Shing; Jan, Gene Eu; Lin, Jheng-Yi

    2016-09-01

    This article presents the VLSI design of a configurable RSA public key cryptosystem supporting the 512-bit, 1024-bit and 2048-bit based on Montgomery algorithm achieving comparable clock cycles of current relevant works but with smaller die size. We use binary method for the modular exponentiation and adopt Montgomery algorithm for the modular multiplication to simplify computational complexity, which, together with the systolic array concept for electric circuit designs effectively, lower the die size. The main architecture of the chip consists of four functional blocks, namely input/output modules, registers module, arithmetic module and control module. We applied the concept of systolic array to design the RSA encryption/decryption chip by using VHDL hardware language and verified using the TSMC/CIC 0.35 m 1P4 M technology. The die area of the 2048-bit RSA chip without the DFT is 3.9 × 3.9 mm2 (4.58 × 4.58 mm2 with DFT). Its average baud rate can reach 10.84 kbps under a 100 MHz clock.

  11. Design and characterization of novel all-solid-state potentiometric sensor array dedicated to physiological measurements.

    Science.gov (United States)

    Toczyłowska-Mamińska, Renata; Kloch, Monika; Zawistowska-Deniziak, Anna; Bala, Agnieszka

    2016-10-01

    A novel construction of all-solid-state potentiometric sensor array designed for physiological measurements has been presented. The planar construction and elimination of liquid phase creates broad opportunities for the modifications in the sensing part of the sensor. The designed construction is based on all-solid-state ion-selective electrodes integrated with the ionic-liquid based reference electrode. Work parameters of the sensor arrays were characterized. It has been shown that presented sensor design indicates high sensitivity (55.2±1mV/dec, 56.3±2mV/dec, 58.4±1mV/dec and 53.5±1mV/pH for sodium-, potassium-, chloride- and pH-selective electrodes, respectively in 10(-5)-10(-1.5)M range of primary ions), low response time (t95 did not exceed 10s), high potential stability (potential drift in 28-h measurement was ca. ±2mV) and potential repeatability ca. ±1mV. The system was successfully applied to the simultaneous determination of K(+), Cl(-), Na(+) and pH in the model physiological solution and for the ion flux studies in human colon epithelium Caco-2 cell line as well.

  12. Design of Frame-transferred Surface Array CCD Imaging System for Dark Objects

    Science.gov (United States)

    Zhang, Yu-heng; Yan, Yi-hua

    2016-01-01

    In order to realize the requirement of low-noise observations of dark objects in deep-space explorations, the design method for a simple and stable space camera imaging system is proposed in this paper. Based on the back-illuminated frame-transferred surface array CCD (CCD47-20AIMO) produced by the British E2V company, the circuitry design is given for the every part of the system. In which the applications of the correlated double-sampling analog-digital converter (AD) and the synchronous dynamic random access memory (SDRAM) can effectively suppress the correlated noise in the image signal. In addition, a drive control method favorable to the adjustment of exposure time is proposed, in the light-sensing stage it provides the exposure time with an independent and adjustable time delay to make the imaging system satisfy the requirement of long exposure time setting. The imaging system adopts the Cyclone III-series EP3C25Q240C8 field programable gate array produced by the Altera company as the kernel control device, and the drives are programmed in modules according to the function of the every device, in favor of transplantation. The simulative and experimental results indicate that the drive circuitry works normally, and that the system design can satisfy the preset requirement.

  13. Design of novel digital reactor protection system based on field programmable gate array

    International Nuclear Information System (INIS)

    A novel design of reactor protection system (RPS) based on field programmable gate array (FPGA) is introduced. It is aided by micro-controller to achieve a kind of device diversity. The 2-out-of-3 voting mechanism is applied. FPGA modules perform the primary protection functions to accelerate the response speed, and avoid the common-mode-failures in software programming. Micro-controllers are able to enhance the communication ability and man-machine interface (MMI), they also accomplish the secondary protection functions

  14. Simulation of g factor for size optimization and coupling in phased array coil design

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    The effect of geometric parameters of the coil element in an array on signal-to-noise ratio (SNR) and g factor of parallel imaging are analyzed, and the optimal parameters for the maximal SNR and minimal g factor are also given in this paper. In addition, the effects of coupling on SNR and g factor have been analyzed. The test results indicate that g factor for parallel imaging is greatly increased and SNR is decreased for coupling. According to the optimization, a six-channel parallel coil has been designed for torso imaging.

  15. Design and Simulation of Routing-switching Protocol Based on Optical Switch Array

    Institute of Scientific and Technical Information of China (English)

    HE Wei; MAO You-ju; LIU Jiang

    2004-01-01

    An optical routing- switching technology based on optical switch array is proposed. The characteristics of the blocking and nonblocking networks are analyzed and compared, odd- even sorting network is used to realize optical routing- switching, relative routing- switching protocol is designed.Simulation test under load shows that it can reduce a blocking effectively and enhance an efficiency of switching. Further, it can transfer the processing and switching within parallel computer from electric domain to optical domain. It can make parallel computer coordinating computing and processing at much more higher speed, storing and transmitting even more efficiently.

  16. An analog bipolar-JFET master slice array for front-end electronics design

    International Nuclear Information System (INIS)

    An analog bipolar-JFET Master Slice Array (MSA) has been designed for implementation of ICs used in nuclear physics front-end electronics. The universal conception of MSAs active and passive elements provides great functional complexity to ICs in using them. The quality of active element parameters, number and values of available resistors and capacitors made it possible to integrate a four channel amplifier-shaper-discriminator with a base line restorer into the MSA die with dimensions 2.7 mmx3.6 mm. Eight-channel ICs can be made by connection of two chips by metal wiring on a wafer

  17. Design and fabrication of adjustable red-green-blue LED light arrays for plant research

    Directory of Open Access Journals (Sweden)

    Kenitz J Dustin

    2005-08-01

    Full Text Available Abstract Background Although specific light attributes, such as color and fluence rate, influence plant growth and development, researchers generally cannot control the fine spectral conditions of artificial plant-growth environments. Plant growth chambers are typically outfitted with fluorescent and/or incandescent fixtures that provide a general spectrum that is accommodating to the human eye and not necessarily supportive to plant development. Many studies over the last several decades, primarily in Arabidopsis thaliana, have clearly shown that variation in light quantity, quality and photoperiod can be manipulated to affect growth and control developmental transitions. Light emitting diodes (LEDs has been used for decades to test plant responses to narrow-bandwidth light. LEDs are particularly well suited for plant growth chambers, as they have an extraordinary life (about 100,000 hours, require little maintenance, and use negligible energy. These factors render LED-based light strategies particularly appropriate for space-biology as well as terrestrial applications. However, there is a need for a versatile and inexpensive LED array platform where individual wavebands can be specifically tuned to produce a series of light combinations consisting of various quantities and qualities of individual wavelengths. Two plans are presented in this report. Results In this technical report we describe the practical construction of tunable red-green-blue LED arrays to support research in plant growth and development. Two light fixture designs and corresponding circuitry are presented. The first is well suited for a laboratory environment for use in a finite area with small plants, such as Arabidopsis. The second is expandable and appropriate for growth chambers. The application of these arrays to early plant developmental studies has been validated with assays of hypocotyl growth inhibition/promotion and phototropic curvature in Arabidopsis seedlings

  18. Design of oligonucleotides for microarrays and perspectives for design of multi-transcriptome arrays

    DEFF Research Database (Denmark)

    Nielsen, Henrik Bjørn; Wernersson, Rasmus; Knudsen, Steen

    2003-01-01

    with an overview of these parameters. We present here a flexible tool named OligoWiz for designing oligonucleotides for multiple purposes. OligoWiz presents a set of parameter scores in a graphical interface to facilitate an overview for the user. Additional custom parameter scores can easily be added......Optimal design of oligonucleotides for microarrays involves tedious and laborious work evaluating potential oligonucleotides relative to a series of parameters. The currently available tools for this purpose are limited in their flexibility and do not present the oligonucleotide designer...... to the program to extend the default parameters: homology, DeltaTm, low-complexity, position and GATC-only. Furthermore we present an analysis of the limitations in designing oligonucleotide sets that can detect transcripts from multiple organisms. OligoWiz is available at www.cbs.dtu.dk/services/OligoWiz/....

  19. Design of On-Chip N-Fold Orbital Angular Momentum Multicasting Using V-Shaped Antenna Array

    Science.gov (United States)

    Du, Jing; Wang, Jian

    2015-01-01

    We design a V-shaped antenna array to realize on-chip multicasting from a single Gaussian beam to four orbital angular momentum (OAM) beams. A pattern search assisted iterative (PSI) algorithm is used to design an optimized continuous phase pattern which is further discretized to generate collinearly superimposed multiple OAM beams. Replacing the designed discrete phase pattern with corresponding V-shaped antennas, on-chip N-fold OAM multicasting is achieved. The designed on-chip 4-fold OAM multicasting exploiting V-shaped antenna array shows favorable operation performance with low crosstalk less than -15 dB. PMID:25951325

  20. Design of acoustic logging signal source of imitation based on field programmable gate array

    Science.gov (United States)

    Zhang, K.; Ju, X. D.; Lu, J. Q.; Men, B. Y.

    2014-08-01

    An acoustic logging signal source of imitation is designed and realized, based on the Field Programmable Gate Array (FPGA), to improve the efficiency of examining and repairing acoustic logging tools during research and field application, and to inspect and verify acoustic receiving circuits and corresponding algorithms. The design of this signal source contains hardware design and software design,and the hardware design uses an FPGA as the control core. Four signals are made first by reading the Random Access Memory (RAM) data which are inside the FPGA, then dealing with the data by digital to analog conversion, amplification, smoothing and so on. Software design uses VHDL, a kind of hardware description language, to program the FPGA. Experiments illustrate that the ratio of signal to noise for the signal source is high, the waveforms are stable, and also its functions of amplitude adjustment, frequency adjustment and delay adjustment are in accord with the characteristics of real acoustic logging waveforms. These adjustments can be used to imitate influences on sonic logging received waveforms caused by many kinds of factors such as spacing and span of acoustic tools, sonic speeds of different layers and fluids, and acoustic attenuations of different cementation planes.

  1. Optimal activation of carboxyl-superparamagnetic iron oxide nanoparticles bioconjugated with antibody using orthogonal array design.

    Science.gov (United States)

    Liu, Lin; Zhang, Xiaoqang; Zhang, Yu; Pu, Yuepu; Yin, Lihong; Tang, Meng; Liu, Hui

    2013-12-01

    This study aims to bioconjugate anti-EMMPRIN monoclonal antibody on the surface of carboxyl-SPIO nanoparticles and to optimize the activated conditions of bioconjugation. Anti-EMMPRIN monoclonal antibody bioconjugated carboxyl-SPIO nanoparticles were performed through a coupling strategy of EDC and sulfo-NHS. The procedure was comprised of two steps by activation of carboxyl-SPIO nanoparticles and conjugation with monoclonal antibody. The optimal activated parameters of bioconjugation were evaluated by single factor design and orthogonal array design. SDS-PAGE analysis and Bradford assay was used for testing and verifying the efficiency of activated conditions obtained from orthogonal array. The results show that pH value, temperature and reaction time were important factors that influence bioconjugated efficiency. The activated parameters with pH value 6.2, temperature 25 degrees C and reaction time 30 min were obviously optimal for activation of carboxyl-SPIO nanoparticles and conjugation with monoclonal EMMPEIN antibody. This coupling strategy for anti-EMMPRIN mAb bioconjugated on SPIO nanoparticles was efficient, and may be further applied in the fields of medical or biological practices. PMID:24266206

  2. A novel stretchable micro-electrode array (SMEA) design for directional stretching of cells

    International Nuclear Information System (INIS)

    Stretchable micro-electrode arrays (SMEAs) are useful tools to study the electrophysiology of living cells seeded on the devices under mechanical stimulation. For such applications, the SMEAs are used as cell culture substrates; therefore, the surface topography and mechanical properties of the devices should be minimally affected by the embedded stretchable electrical interconnects. In this paper, a novel design and micro-fabrication technology for a pneumatically actuated SMEA are presented to achieve stretchability with minimal surface area dedicated to the electrical interconnects and a well-defined surface strain distribution combined with integrated diverse micro-patterns to enable alignment and directional stretching of cells. The special mechanical design also enables the SMEA to have a prolonged electro-mechanical fatigue life time required for long-term cyclic stretching of the cell cultures (stable resistance of electrical interconnects for more than 160 thousand cycles of 20% stretching and relaxing). The proposed fabrication method is based on the state of the art micro-fabrication techniques and materials and circumvents the processing problems associated with using unconventional methods and materials to fabricate stretchable electrode arrays. The electrochemical impedance spectroscopy characterization of the SMEA shows 4.5 MΩ impedance magnitude at 1 kHz for a TiN electrode 12 um in diameter. Cell culture experiments demonstrate the robustness of the SMEAs for long-term culturing experiments and compatibility with inverted fluorescent microscopy. (paper)

  3. Design and calibration of a six-axis MEMS sensor array for use in scoliosis correction surgery

    International Nuclear Information System (INIS)

    A six-axis sensor array has been developed to quantify the 3D force and moment loads applied in scoliosis correction surgery. Initially this device was developed to be applied during scoliosis correction surgery and augmented onto existing surgical instrumentation, however, use as a general load sensor is also feasible. The development has included the design, microfabrication, deployment and calibration of a sensor array. The sensor array consists of four membrane devices, each containing piezoresistive sensing elements, generating a total of 16 differential voltage outputs. The calibration procedure has made use of a custom built load application frame, which allows quantified forces and moments to be applied and compared to the outputs from the sensor array. Linear or non-linear calibration equations are generated to convert the voltage outputs from the sensor array back into 3D force and moment information for display or analysis. (papers)

  4. Design and calibration of a six-axis MEMS sensor array for use in scoliosis correction surgery

    Science.gov (United States)

    Benfield, David; Yue, Shichao; Lou, Edmond; Moussa, Walied A.

    2014-08-01

    A six-axis sensor array has been developed to quantify the 3D force and moment loads applied in scoliosis correction surgery. Initially this device was developed to be applied during scoliosis correction surgery and augmented onto existing surgical instrumentation, however, use as a general load sensor is also feasible. The development has included the design, microfabrication, deployment and calibration of a sensor array. The sensor array consists of four membrane devices, each containing piezoresistive sensing elements, generating a total of 16 differential voltage outputs. The calibration procedure has made use of a custom built load application frame, which allows quantified forces and moments to be applied and compared to the outputs from the sensor array. Linear or non-linear calibration equations are generated to convert the voltage outputs from the sensor array back into 3D force and moment information for display or analysis.

  5. Design of primary optics for LED chip array in road lighting application.

    Science.gov (United States)

    Wang, Shang; Wang, Kai; Chen, Fei; Liu, Sheng

    2011-07-01

    In this study, we proposed an effective optical design method to solve the problem of prescribed illuminance for LED chip array packaging (LCAP). With this method, light energy redistribution can be obtained by optimizing meshing parameters on the target. Not only can it deal with light controlling of extended source, but also improve the uniformity of both illuminance and luminance for road lighting as well. As an example, we designed a 16W LED packaging with a smooth primary freeform lens for general road lighting. The simulation results demonstrated that optical performance of this lens can meet the requirements of Commission Internationale del'Eclairage (CIE) quite well. Longitudinal and overall uniformities are 0.7 and 0.5 respectively, and relative optical efficiency of luminaire can be enhanced 19.6% in theory compared with traditional optics. PMID:21747539

  6. Design and optimization of multi-class series-parallel linear electromagnetic array artificial muscle.

    Science.gov (United States)

    Li, Jing; Ji, Zhenyu; Shi, Xuetao; You, Fusheng; Fu, Feng; Liu, Ruigang; Xia, Junying; Wang, Nan; Bai, Jing; Wang, Zhanxi; Qin, Xiansheng; Dong, Xiuzhen

    2014-01-01

    Skeletal muscle exhibiting complex and excellent precision has evolved for millions of years. Skeletal muscle has better performance and simpler structure compared with existing driving modes. Artificial muscle may be designed by analyzing and imitating properties and structure of skeletal muscle based on bionics, which has been focused on by bionic researchers, and a structure mode of linear electromagnetic array artificial muscle has been designed in this paper. Half sarcomere is the minimum unit of artificial muscle and electromagnetic model has been built. The structural parameters of artificial half sarcomere actuator were optimized to achieve better movement performance. Experimental results show that artificial half sarcomere actuator possesses great motion performance such as high response speed, great acceleration, small weight and size, robustness, etc., which presents a promising application prospect of artificial half sarcomere actuator.

  7. Design and Simulation of Array of Rectangular Micro Cantilevers Piezoelectric Energy Harvester

    Directory of Open Access Journals (Sweden)

    Komal Kumari

    2016-09-01

    Full Text Available This paper presents the design, analysis and simulation of MEMS based array of bimorph rectangular microcantilever piezoelectric energy harvester structure with and without tip mass, to analyze their sensitivity. The microcantilever beams are made up of piezoelectric material and Aluminium as a substrate material. The analytical simulation of design is done by FEM (COMSOL Multiphysics. The simulation results of bimorph cantilever structure, applied force of 0.1 N and obtained end displacement and electric potential developed are given. The analytical model of the cantilever beam will be analyzed and the process of its construction will be discussed. The changes in the sensitivity of a cantilever beam with respect to change in its shape for the same applied force of 0.1N are denoted.

  8. Chromosomal aberrations detected by comparative genomic hybridization technique (CGH in invasive ductal carcinoma of breast

    Directory of Open Access Journals (Sweden)

    Nooshiravanpour P

    2007-10-01

    Full Text Available Background: Nonlethal genetic damage is the basis for carcinogenesis. As various gene aberrations accumulate, malignant tumors are formed, regardless of whether the genetic damage is subtle or large enough to be distinguished in a karyotype. The study of chromosomal changes in tumor cells is important in the identification of oncogenes and tumor suppressor genes by molecular cloning of genes in the vicinity of chromosomal aberrations. Furthermore, some specific aberrations can be of great diagnostic and prognostic value. Comparative genomic hybridization (CGH is used to screen the entire genome for the detection and/or location chromosomal copy number changes.Methods: In this study, frozen sections of 20 primary breast tumors diagnosed as invasive ductal carcinoma from the Cancer Institute of Imam Khomeini Hospital, Tehran, Iran, were studied by CGH to detect chromosomal aberrations. We compared histopathological and immunohistochemical findings.Results: Hybridization in four of the cases was not optimal for CGH analysis and they were excluded from the study. DNA copy number changes were detected in 12 (75% of the remaining 16 cases. Twenty-one instances of chromosomal aberrations were detected in total, including: +1q, +17q, +8q, +20q, -13q, -11q, -22q, -1p, -16q, -8p. The most frequent were +1q, +17q, +8q, -13q, similar to other studies. In three cases, we detected -13q, which is associated with axillary lymph node metastasis and was reported in one previous study. The mean numbers of chromosomal aberrations per tumor in metastatic and nonmetastatic tumors was 1.5 and 1, respectively. No other association between detected chromosomal aberrations and histopathological and immunohistochemical findings were seen.Conclusion: Since intermediately to widely invasive carcinomas are more likely to have chromosomal aberrations, CGH can be a valuable prognostic tool. Furthermore, CGH can be used to detect targeting molecules within novel amplifications

  9. Design and synthesis of flexible switching 1 × 2 antenna array on Kapton substrate

    Science.gov (United States)

    Georges Rabobason, Yvon; Rigas, Grigorios; Swaisaenyakorn, Srijittar; Mirkhaydarov, Bobur; Ravelo, Blaise; Shkunov, Maxim; Young, Paul; Benjelloun, Nabil

    2016-06-01

    Flexible front- and back-end RF/analogue system antennas were recently emerged. However, little flexible antenna system design is available so far, in planar hybrid technology with surface mounted components. This paper describes the design feasibility of flexible switching 1 × 2 antenna array system. It acts as a switching antenna implemented in hexapole configuration. The system is comprised of a key element RF switch terminated by two identical patch antennas associated to half-wave elementary transmission lines (TLs). A detailed theory illustrating the global S-parameter model determination in function of the given RF-switch return and insertion losses is established. In difference to the conventional microwave circuit theory, the proposed equivalent S-parameter model is originally built with the non-standard optimized antenna load. Thus, the synthesis method of the terminal antenna input impedance and the output access line characteristic impedance is formulated in function of the specified return and optimal transmission losses. The design method and theoretical approach feasibility is verified with the demonstrator of flexible switching 1 × 2 antenna array printed on Kapton substrate. The circuit prototype is implemented in hybrid planar technology integrating patch antenna operating at about 6 GHz and a packaged GaAs RF switch associated to the RF/DC signal decoupling accessory mounted surface components. Simulations of the designed circuit transmission and isolation losses from 5.5 GHz to 7 GHz were carried out by using the commercial RF switch S-parameter touchstone model provided by the manufacturer. The simulated and measured return losses are compared and discussed. Then, the measured radiation patterns confirm the proposed switched antenna concept feasibility.

  10. Design Methodology of a Dual-Halbach Array Linear Actuator with Thermal-Electromagnetic Coupling.

    Science.gov (United States)

    Eckert, Paulo Roberto; Flores Filho, Aly Ferreira; Perondi, Eduardo; Ferri, Jeferson; Goltz, Evandro

    2016-01-01

    This paper proposes a design methodology for linear actuators, considering thermal and electromagnetic coupling with geometrical and temperature constraints, that maximizes force density and minimizes force ripple. The method allows defining an actuator for given specifications in a step-by-step way so that requirements are met and the temperature within the device is maintained under or equal to its maximum allowed for continuous operation. According to the proposed method, the electromagnetic and thermal models are built with quasi-static parametric finite element models. The methodology was successfully applied to the design of a linear cylindrical actuator with a dual quasi-Halbach array of permanent magnets and a moving-coil. The actuator can produce an axial force of 120 N and a stroke of 80 mm. The paper also presents a comparative analysis between results obtained considering only an electromagnetic model and the thermal-electromagnetic coupled model. This comparison shows that the final designs for both cases differ significantly, especially regarding its active volume and its electrical and magnetic loading. Although in this paper the methodology was employed to design a specific actuator, its structure can be used to design a wide range of linear devices if the parametric models are adjusted for each particular actuator.

  11. Design Methodology of a Dual-Halbach Array Linear Actuator with Thermal-Electromagnetic Coupling.

    Science.gov (United States)

    Eckert, Paulo Roberto; Flores Filho, Aly Ferreira; Perondi, Eduardo; Ferri, Jeferson; Goltz, Evandro

    2016-01-01

    This paper proposes a design methodology for linear actuators, considering thermal and electromagnetic coupling with geometrical and temperature constraints, that maximizes force density and minimizes force ripple. The method allows defining an actuator for given specifications in a step-by-step way so that requirements are met and the temperature within the device is maintained under or equal to its maximum allowed for continuous operation. According to the proposed method, the electromagnetic and thermal models are built with quasi-static parametric finite element models. The methodology was successfully applied to the design of a linear cylindrical actuator with a dual quasi-Halbach array of permanent magnets and a moving-coil. The actuator can produce an axial force of 120 N and a stroke of 80 mm. The paper also presents a comparative analysis between results obtained considering only an electromagnetic model and the thermal-electromagnetic coupled model. This comparison shows that the final designs for both cases differ significantly, especially regarding its active volume and its electrical and magnetic loading. Although in this paper the methodology was employed to design a specific actuator, its structure can be used to design a wide range of linear devices if the parametric models are adjusted for each particular actuator. PMID:26978370

  12. Model based on-chip 13bits ADC design dedicated to uncooled infrared focal plane arrays

    Science.gov (United States)

    Dupont, Benoit; Robert, Patrick; Dupret, Antoine; Villard, Patrick; Pochic, David

    2007-10-01

    This paper presents an on-chip 13 bits 10 M/S Analog to Digital Converter (ADC) specifically designed for infrared bolometric image sensor. Bolometric infrared sensors are MEMs based thermal sensors, which covers a large spectrum of infrared applications, ranging from night vision to predictive industrial maintenance and medical imaging. With the current move towards submicron technologies, the demand for more integrated, smarter sensors and microsystems has dramatically increased. This trend has strengthened the need of on-chip ADC as the interface between the analog core and the digital processing electronic. However designing an on-chip ADC dedicated to focal plane array raises many questions about its architecture and its performance requirements. To take into account those specific needs, a high level model has been developed prior to the actual design. In this paper, we present the trade-offs of ADC design linked to infrared key performance parameters and bolometric technology detection method. The original development scheme, based on system level modeling, is also discussed. Finally we present the actual design and the measured performances.

  13. Development of accelerated test design for service-life prediction of solar array at Mead, Nebraska. Quarterly report

    Energy Technology Data Exchange (ETDEWEB)

    Gaines, G.B.; Thomas, R.E.; Noel, G.T.; Shilliday, T.S.; Wood, V.E.; Carmichael, D.C.

    1978-11-03

    As a significant beginning in applying accelerated tests to solar arrays for life-prediction purposes, this study is directed toward (a) developing a plan for predicting the service life of a specific solar array in a specific geographic site - viz., the 25-KW flat-plate array installed near Mead, Nebraska, and (b) developing technical information from laboratory and field measurements for designing an accelerated test that can be carried out in 2 years and have predictive validity for a service life as long as 20 years. Status of the program is described.

  14. Quadrature transmit array design using single-feed circularly polarized patch antenna for parallel transmission in MR imaging

    OpenAIRE

    Pang, Yong; Yu, Baiying; Daniel B Vigneron; Zhang, Xiaoliang

    2014-01-01

    Quadrature coils are often desired in MR applications because they can improve MR sensitivity and also reduce excitation power. In this work, we propose, for the first time, a quadrature array design strategy for parallel transmission at 298 MHz using single-feed circularly polarized (CP) patch antenna technique. Each array element is a nearly square ring microstrip antenna and is fed at a point on the diagonal of the antenna to generate quadrature magnetic fields. Compared with conventional ...

  15. Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC and its application for DNA methylation analysis

    Directory of Open Access Journals (Sweden)

    Ottaviani Diego

    2008-05-01

    Full Text Available Abstract Background The major histocompatibility complex (MHC is essential for human immunity and is highly associated with common diseases, including cancer. While the genetics of the MHC has been studied intensively for many decades, very little is known about the epigenetics of this most polymorphic and disease-associated region of the genome. Methods To facilitate comprehensive epigenetic analyses of this region, we have generated a genomic tiling array of 2 Kb resolution covering the entire 4 Mb MHC region. The array has been designed to be compatible with chromatin immunoprecipitation (ChIP, methylated DNA immunoprecipitation (MeDIP, array comparative genomic hybridization (aCGH and expression profiling, including of non-coding RNAs. The array comprises 7832 features, consisting of two replicates of both forward and reverse strands of MHC amplicons and appropriate controls. Results Using MeDIP, we demonstrate the application of the MHC array for DNA methylation profiling and the identification of tissue-specific differentially methylated regions (tDMRs. Based on the analysis of two tissues and two cell types, we identified 90 tDMRs within the MHC and describe their characterisation. Conclusion A tiling array covering the MHC region was developed and validated. Its successful application for DNA methylation profiling indicates that this array represents a useful tool for molecular analyses of the MHC in the context of medical genomics.

  16. Design and analysis of a novel virtual gyroscope with multi-gyroscope and accelerometer array

    Science.gov (United States)

    Luo, Zhang; Liu, Chaojun; Yu, Shuai; Zhang, Shengzhi; Liu, Sheng

    2016-08-01

    A new virtual gyroscope with multi-gyroscope and accelerometer array (MGAA) is proposed in this article for improving the performance of angular rate measurement. Outputs of the virtual gyroscope are obtained by merging the signals from gyroscopes and accelerometers through a novel Kalman filter, which intentionally takes the consideration of the MEMS gyroscope error model and kinematics theory of rigid body. A typical configuration of the virtual gyroscope, consisting of four accelerometers and three gyroscopes mounted on designated positions, is initiated to verify the feasibility of the virtual gyroscope with MGAA. Static test and dynamic test are performed subsequently to evaluate its performance. The angular random walk (ARW) and bias instability, two static performance parameters of gyroscope, are reduced from 0.019°/√s and 14.4°/h to 0.0074°/√s and 8.7°/h, respectively. The average root mean square error (RMSE) is reduced from 0.274°/s to 0.133°/s under dynamic test. Compared with the published multi-gyroscope array method, the virtual gyroscope proposed here has a better performance both in static and dynamic tests, with improvement factors of ARW and RMSE about 44.1% and 44.5% higher, respectively.

  17. G3 Assisted Rational Design of Chemical Sensor Array Using Carbonitrile Neutral Receptors

    Directory of Open Access Journals (Sweden)

    Yatimah Alias

    2013-10-01

    Full Text Available Combined computational and experimental strategies for the systematic design of chemical sensor arrays using carbonitrile neutral receptors are presented. Binding energies of acetonitrile, n-pentylcarbonitrile and malononitrile with Ca(II, Mg(II, Be(II and H+ have been investigated with the B3LYP, G3, CBS-QB3, G4 and MQZVP methods, showing a general trend H+ > Be(II > Mg(II > Ca(II. Hydrogen bonding, donor-acceptor and cation-lone pair electron simple models were employed in evaluating the performance of computational methods. Mg(II is bound to acetonitrile in water by 12.5 kcal/mol, and in the gas phase the receptor is more strongly bound by 33.3 kcal/mol to Mg(II compared to Ca(II. Interaction of bound cations with carbonitrile reduces the energies of the MOs involved in the proposed σ-p conjugated network. The planar malononitrile-Be(II complex possibly involves a π-network with a cationic methylene carbon. Fabricated potentiometric chemical sensors show distinct signal patterns that can be exploited in sensor array applications.

  18. A novel design of infrared focal plane array with digital read out interface

    Science.gov (United States)

    Liu, Xiaoyang; Ding, Ruijun; Lu, Wei; Zhou, Chun

    2010-10-01

    Infrared focal plane array (IRFPA) with digital read out interface is a key sign of the third generation IRFPA, which plays an important role in the reliability and miniaturization of infrared systems. A readout integrated circuit (ROIC) of IRFPA with digital readout interface based on dual ramp single slope (DRSS) analog to digital converter (ADC) architecture is presented in the paper. The design is realized using shared ADCs in column-wise and these ADCs are consisted of simplified DRSS architecture and shared units. Sample, conversion and readout are proceeded simultaneously in order to adapt large scale and high readout frame rate application. This circuit also shows many advantages, including small area and low power consumption. Simulation result shows that this architecture can be expand to 320×256 pixel array with a frame rate of 100 frames per second or a larger size whit lower frame rate, the quantized resolution of this circuit is 12 bit, and the analog power consumption is only 17μw per ADC.

  19. Design of DSP-based high-power digital solar array simulator

    Science.gov (United States)

    Zhang, Yang; Liu, Zhilong; Tong, Weichao; Feng, Jian; Ji, Yibo

    2013-12-01

    To satisfy rigid performance specifications, a feedback control was presented for zoom optical lens plants. With the increasing of global energy consumption, research of the photovoltaic(PV) systems get more and more attention. Research of the digital high-power solar array simulator provides technical support for high-power grid-connected PV systems research.This paper introduces a design scheme of the high-power digital solar array simulator based on TMS320F28335. A DC-DC full-bridge topology was used in the system's main circuit. The switching frequency of IGBT is 25kHz.Maximum output voltage is 900V. Maximum output current is 20A. Simulator can be pre-stored solar panel IV curves.The curve is composed of 128 discrete points .When the system was running, the main circuit voltage and current values was feedback to the DSP by the voltage and current sensors in real-time. Through incremental PI,DSP control the simulator in the closed-loop control system. Experimental data show that Simulator output voltage and current follow a preset solar panels IV curve. In connection with the formation of high-power inverter, the system becomes gridconnected PV system. The inverter can find the simulator's maximum power point and the output power can be stabilized at the maximum power point (MPP).

  20. Design of Non-Uniform Linear Antenna Arrays Using DolphChebyshev and Binomial Methods

    Directory of Open Access Journals (Sweden)

    Jean-François D. Essiben

    2015-08-01

    Full Text Available This paper explores the analytical methods of synthesizing linear antenna arrays. The synthesis employed is based on non-uniform methods. In particular, the Dolph-Chebyshev and binomial methods are used, so as to improve the directivity of the array and to reduce the level of the secondary lobes by adjusting the geometrical and electric parameters of the array. The radiation patterns, the directivity, and the array factors of the uniform and the non-uniform methods are presented. It is shown that the Chebyshev arrays have better directivity than binomial arrays for the same number of elements and separation distance, while binomial arrays have very low side lobes compared with Chebyshev and uniform excitation arrays. Finally, numerical results of both methods are analyzed and compared.

  1. Progress in Monte Carlo design and optimization of the Cherenkov Telescope Array

    CERN Document Server

    Bernlöhr, K; Becherini, Y; Bigas, O Blanch; Bouvier, A; Carmona, E; Colin, P; Decerprit, G; Di Pierro, F; Dubois, F; Farnier, C; Funk, S; Hermann, G; Hinton, J A; Humensky, T B; Jogler, T; Khélifi, B; Kihm, T; Komin, N; Lenain, J -P; López-Coto, R; Maier, G; Mazin, D; Medina, M C; Moralejo, A; Moderski, R; Nolan, S J; Ohm, S; Wilhelmi, E de Oña; Parsons, R D; Arribas, M Paz; Pedaletti, G; Pita, S; Prokoph, H; Rulten, C B; Schwanke, U; Shayduk, M; Stamatescu, V; Vallania, P; Vorobiov, S; Wischnewski, R; Wood, M; Yoshikoshi, T; Zech, A

    2013-01-01

    The Cherenkov Telescope Array (CTA) will be an instrument covering a wide energy range in very-high-energy (VHE) gamma rays. CTA will include several types of telescopes, in order to optimize the performance over the whole energy range. Both large-scale Monte Carlo (MC) simulations of CTA super-sets (including many different possible CTA layouts as sub-sets) and smaller-scale simulations dedicated to individual aspects were carried out and are on-going. We summarize results of the prior round of large-scale simulations, show where the design has now evolved beyond the conservative assumptions of the prior round and present first results from the on-going new round of MC simulations.

  2. Design Concepts For A Long Pulse Upgrade For The DIII-D Fast Wave Antenna Array

    Energy Technology Data Exchange (ETDEWEB)

    Ryan, Philip Michael [ORNL; Baity Jr, F Wallace [ORNL; Caughman, John B [ORNL; Goulding, Richard Howell [ORNL; Hosea, J. [Princeton Plasma Physics Laboratory (PPPL); Greenough, Nevell [Princeton Plasma Physics Laboratory (PPPL); Nagy, Alex [Princeton Plasma Physics Laboratory (PPPL); Pinsker, R. [General Atomics; Rasmussen, David A [ORNL

    2009-01-01

    A goal in the 5-year plan for the fast wave program on DIII-D is to couple a total of 3.6 MW of RF power into a long pulse, H-mode plasma for central electron heating. The present short-pulse 285/300 antenna array would need to be replaced with one capable of at least 1.2 MW, 10 s operation at 60 MHz into an H-mode (low resistive loading) plasma condition. The primary design under consideration uses a poloidally-segmented strap (3 sections) for reduced strap voltage near the plasma/Faraday screen region. Internal capacitance makes the antenna structure self-resonant at 60 MHz, strongly reducing peak E-fields in the vacuum coax and feed throughs.

  3. Designing shielded radio-frequency phased-array coils for magnetic resonance imaging

    Institute of Scientific and Technical Information of China (English)

    Xu Wen-Long; Zhang Ju-Cheng; Li Xia; Xu Bing-Qiao; Tao Gui-Sheng

    2013-01-01

    In this paper,an approach to the design of shielded radio-frequency (RF) phased-array coils for magnetic resonance imaging (MRI) is proposed.The target field method is used to find current densities distributed on primary and shield coils.The stream function technique is used to discretize current densities and to obtain the winding patterns of the coils.The corresponding highly ill-conditioned integral equation is solved by the Tikhonov regularization with a penalty function related to the minimum curvature.To balance the simplicity and smoothness with the homogeneity of the magnetic field of the coil's winding pattern,the selection of a penalty factor is discussed in detail.

  4. Design of readout circuit for microcantilever-based ripple uncooled infrared focal plane arrays

    Science.gov (United States)

    Cao, Junmin; Chen, Zhongjian; Lu, Wengao; Zhang, Yacong; Lei, Ke; Zhao, Baoying

    2009-07-01

    A readout integrated circuit (ROIC) for uncooled microcantilever infrared focal plane arrays (IRFPAs) based on capacitive readout is proposed. The ROIC is optimized according to noise modeling and analysis to reduce noise. An experimental chip of 16×16 FPAs readout circuit has been designed and fabricated using 0.35um CMOS technology. The measurement results showed that the power dissipation is 16.5mW from a 5V supply voltage at 50Hz frame rate, the linearity is 99.2% at the typical mode; the uniformity is larger than 97% and the equivalent noise charge (ENC) is below 150e. It is believed that the ROIC has a great potential in the applications of large-scale micro-cantilever-based uncooled IRFPAs.

  5. Application of Correlation and Coherency Methods to Naqu Seismic Array Design

    Institute of Scientific and Technical Information of China (English)

    Hao Chunyue; Zheng Zhong

    2006-01-01

    The waveform data recorded during the site survey of the Naqu seismic array in Aug. 2004 were processed by the author. According to the relativity of site pairs in time and frequency domains, the reference values of the inner ring and outer ring were determined. The author evaluated the array deployed for site survey as the primary array, and the results show that the resolution of the primary array is high enough to locate earthquakes precisely.

  6. Design Studies for a VUV--Soft X-ray Free-Electron Laser Array

    International Nuclear Information System (INIS)

    Several recent reports have identified the scientific requirements for a future soft X-ray light source, and a high-repetition-rate free-electron laser (FEL) facility responsive to them is being studied at Lawrence Berkeley National Laboratory (LBNL). The facility is based on a continuous-wave (CW) superconducting linear accelerator with beam supplied by a high-brightness, high-repetition-rate photocathode electron gun operating in CW mode, and on an array of FELs to which the accelerated beam is distributed, each operating at high repetition rate and with even pulse spacing. Dependent on the experimental requirements, the individualFELs may be configured for either self-amplified spontaneous emission (SASE), seeded highgain harmonic generation (HGHG), echo-enabled harmonic generation (EEHG), or oscillator mode of operation, and will produce high peak and average brightness x-rays with a flexible pulse format ranging from sub-femtoseconds to hundreds of femtoseconds. This new light source would serve a broad community of scientists in many areas of research, similar to existing utilization of storage ring based light sources. To reduce technical risks and constructioncosts, accelerator research, development, and design studies at LBNL target the most critical components and systems of the facility. We are developing a high-repetition-rate low-emittance electron gun, high quantum efficiency photocathodes, and have embarked on design and optimization of the electron beam accelerator, FEL switchyard, and array of FELs. We continue our work on precision timing and synchronization systems critical for time-resolved experiments using pump-probe techniques.

  7. Improved design of superconductor/normal conductor/superconductor Josephson junction series arrays for an ac Josephson voltage standard

    International Nuclear Information System (INIS)

    Superconductor/normal conductor/superconductor (SNS) Josephson junction series arrays are the subject of research for the realization of a Josephson arbitrary waveform synthesizer (JAWS) based on pulse-driven Josephson junctions. Lumped arrays of SNS Josephson junctions are investigated for this application at the Physikalisch-Technische Bundesanstalt (PTB). The series arrays consisting of Nb/HfTi/Nb SNS junctions are embedded in the center line of a coplanar waveguide transmission line. To increase the ac output voltage, the Josephson junction series array is designed as a meander-like structure. A broadband response of the arrays is required for the complex pulse operation of the JAWS. The arrays are investigated by dc measurements and for test purposes under continuous microwave irradiation at different frequencies. Flat Shapiro steps were measured for meander-like series arrays with 21 parallel lines containing up to 10 752 junctions. A sine wave with a frequency of 2.5 kHz and a peak voltage of 214 mVPeak was synthesized under pulse mode operation

  8. Transport and collision dynamics in periodic asymmetric obstacle arrays: Rational design of microfluidic rare-cell immunocapture devices

    Science.gov (United States)

    Gleghorn, Jason P.; Smith, James P.; Kirby, Brian J.

    2013-09-01

    Microfluidic obstacle arrays have been used in numerous applications, and their ability to sort particles or capture rare cells from complex samples has broad and impactful applications in biology and medicine. We have investigated the transport and collision dynamics of particles in periodic obstacle arrays to guide the design of convective, rather than diffusive, transport-based immunocapture microdevices. Ballistic and full computational fluid dynamics simulations are used to understand the collision modes that evolve in cylindrical obstacle arrays with various geometries. We identify previously unrecognized collision mode structures and differential size-based collision frequencies that emerge from these arrays. Previous descriptions of transverse displacements that assume unidirectional flow in these obstacle arrays cannot capture mode transitions properly as these descriptions fail to capture the dependence of the mode transitions on column spacing and the attendant change in the flow field. Using these analytical and computational simulations, we elucidate design parameters that induce high collision rates for all particles larger than a threshold size or selectively increase collision frequencies for a narrow range of particle sizes within a polydisperse population. Furthermore, we investigate how the particle Péclet number affects collision dynamics and mode transitions and demonstrate that experimental observations from various obstacle array geometries are well described by our computational model.

  9. Planar ESPAR Array Design with Nonsymmetrical Pattern by Means of Finite-Element Method, Domain Decomposition, and Spherical Wave Expansion

    Directory of Open Access Journals (Sweden)

    Jesús García

    2012-01-01

    Full Text Available The application of a 3D domain decomposition finite-element and spherical mode expansion for the design of planar ESPAR (electronically steerable passive array radiator made with probe-fed circular microstrip patches is presented in this work. A global generalized scattering matrix (GSM in terms of spherical modes is obtained analytically from the GSM of the isolated patches by using rotation and translation properties of spherical waves. The whole behaviour of the array is characterized including all the mutual coupling effects between its elements. This procedure has been firstly validated by analyzing an array of monopoles on a ground plane, and then it has been applied to synthesize a prescribed radiation pattern optimizing the reactive loads connected to the feeding ports of the array of circular patches by means of a genetic algorithm.

  10. Interference testing methods of large astronomical mirrors base on lenses and CGH wavefront correctors

    Science.gov (United States)

    Abdulkadyrov, Magomed A.; Belousov, Sergey P.; Patrikeev, Vladimir E.; Semenov, Alexandr P.

    2010-07-01

    Since last years and at present days LZOS, JSC has been producing a range of primary mirrors of astronomical telescopes with diameter more than 1m under contracts with foreign companies. Simultaneous testing of an aspherical surface figure by means of a lens corrector and CGH (computer generated hologram) corrector, testing of the corrector using the CGH allow challenging the task of definite testing of the mirrors surfaces figure. The results of successful figuring of the mirrors with diameter up to 4m like VISTA Project (Southern European Observatory), TNT (Thai National telescope, Australia - Thailand), LCO telescopes (Las Cumbres Observatory, USA; Russian national projects and meeting these mirrors specifications' requirements are all considered as the sufficient evidence.

  11. Investigation of error compensation in CGH-based form testing of aspheres

    Science.gov (United States)

    Stuerwald, S.; Brill, N.; Schmitt, R.

    2014-05-01

    Interferometric form testing using computer generated holograms is one of the main full-field measurement techniques. Till now, various modified measurement setups for optical form testing interferometry have been presented. Currently, typical form deviations in the region of several tens of nanometers occur in case of the widely used computer generated hologram (CGH) based interferometric form testing. Deviations occur due to a non-perfect alignment of the computer generated hologram (CGH) relative to the transmission sphere (Fizeau objective) and also of the asphere relative to the testing wavefront. Thus, measurement results are user and setup dependent which results in an unsatisfactory reproducibility of the form errors. In case of aligning a CGH, this usually requires a minimization of the spatial frequency of the fringe pattern by an operator. Finding the ideal position however often cannot be performed with sufficient accuracy by the operator as the position of minimum spatial fringe density is usually not unique. Therefore, the scientific and technical objectives of this paper comprise the development of a simulation based approach to explain and quantify the experimental errors due to misalignment of the specimen towards a computer generated hologram in an optical form testing measurement system. A further step is the programming of an iterative method to realize a virtual optimised realignment of the system on the basis of Zernike polynomial decomposition which should allow the calculation of the measured form for an ideal alignment and thus the subtraction of the alignment based form error. Different analysis approaches are investigated with regard to the final accuracy and reproducibility. To validate the theoretical models a series of systematic experiments is performed with hexapod-positioning systems in order to allow an exact and reproducible positioning of the optical CGH-based setup.

  12. The Design of Monolithic AC-coupled 1-Dimensional Voltage-Controlled-Oscillators (VCOs) Phased-array Network

    Science.gov (United States)

    Lie, Donald Y. C.; Lopez, J.

    2011-04-01

    A fully monolithic 1-Dimensional (1-D) AC-coupled Voltage-Controlled-Oscillators (VCOs) phased-array network design will be presented in this paper. This radio-frequency (RF) VCO array integrates on-chip inductors, varactors and bias current sources and it contains an odd number of VCOs AC-coupled through on-chip switchable resistor networks using MOSFETs. The measured results and SPICE simulated performance of the monolithic unit cell VCO agree reasonably well. Realistic circuit simulations in IBM 7HP 0.18 um BiCMOS design kit indicate promising results of the 1-D coupled-VCO array by showing the design can control the phasing of this on-chip VCO-array by means of tuning the edge elements and/or by varying the coupling strength via different resistor values using the on-chip MOSFET switches. Simulation data shows that it can offer high directivity and a possible element-to-element phase tuning arrangement that allows a ˜±20-30° degree coverage from broadside without the need for phase shifters or additional circuitry complexity. This AC-coupled 1-D VCO array, therefore, shows great potential for RF active antennas applications to perform wide angle beam steering for the highly used S-band.

  13. 相控阵天线阵面测试平台软件设计%Software Design of Phased Array Antenna Array Testing Platform

    Institute of Scientific and Technical Information of China (English)

    万东成; 章国宝

    2016-01-01

    In modern large phased array radar,the number of transmitter and receiver components in the antenna is large. The system test is very cumbersome.In order to simplify the testing process and improve the efficiency of manual test,in the array front and TR components testing process,combining with computer software & hardware technologies,data processing technology,automatic test technology,by means of network communication,a set of antenna array surface general test plat-form is designed in this paper.%现代大型相控阵雷达中,天线内的发射和接收(TR)组件数目庞大,系统测试工作十分繁琐,为简化测试过程,提高测试效率,在阵面以及TR组件测试过程中,综合运用计算机软硬件技术、数据处理技术、自动测试技术,采用网络通信手段,设计了一套天线阵面通用测试平台。

  14. The Design of FPGA-based Array CCD Sensor Drive System

    Directory of Open Access Journals (Sweden)

    Chengtao Cai

    2014-01-01

    Full Text Available CCD Sensor is the crutial equipment for environment perception which is widely used in various fields such as surveilliance,vision navigation and machine vision. The commercial CCD device has been encapsulated the sensor driver inside which is not opened for secondary development. Even this mode facilitate the usage but it really can not content the customizable need. For solving this challenging but imperative issue, we designed a novel CCD sensor driver system which implement the efficient and effective image acquisition task in customizing approach. The working principle and driving timing sequence about ICX625AQA the interline CCD image sensor used in our system are discussed in detail. For handling with this data intensive task, a high performance Field Programmable GateArray (FPGA controller is used for data allocation and translation, the peripheral circuits including AD9974 and CXD3400 drive interface which process the horizontal signal and vertical signal, respectively. The designed system proposed at the end of this paper.

  15. Design, Fabrication, and Packaging of an Integrated, Wirelessly-Powered Optrode Array for Optogenetics Application

    Directory of Open Access Journals (Sweden)

    Wen eLi

    2015-05-01

    Full Text Available The recent development of optogenetics has created an increased demand for advancing engineering tools for optical modulation of neural circuitry. This paper details the design, fabrication, integration, and packaging procedures of a wirelessly-powered, light emitting diode (LED coupled optrode neural interface for optogenetic studies. The LED-coupled optrode array employs microscale LED (μLED dies and polymer-based microwaveguides to deliver light into multi-level cortical networks, coupled with microelectrodes to record spontaneous changes in neural activity. An integrated, implantable, switched-capacitor based stimulator (SCS system provides high instantaneous power to the μLEDs through an inductive link to emit sufficient light and evoke neural activities. The presented system is mechanically flexible, biocompatible, miniaturized, and lightweight, suitable for chronic implantation in small freely behaving animals. The design of this system is scalable and its manufacturing is cost effective through batch fabrication using microelectromechanical systems (MEMS technology. It can be adopted by other groups and customized for specific needs of individual experiments.

  16. Fabrication and design of various dimensions of multi-step ashperical microlens arrays for OLED package

    Science.gov (United States)

    Pan, C. T.; Chen, Y. C.; Chen, M. F.; Hsu, Y. C.

    2011-06-01

    An optical film with aspherical microlens array (A-MLA) by using multi-step lithography process for an OLED (Organic Light-Emitting Diode) package is fabricated, by which method the luminance of OLEDs can be enhanced. The method of design and fabrication of an A-MLA is explored in this study. In the design process, various parameters of an A-MLA such as curved profiles, layout template and dimensions of microlens are analyzed and characterized. Curved profiles include hyperbola, parabola, ellipse and sphere; and layout templates include square, hexagon, triangle and tangential circle. The profile of the A-MLA was determined by using a commercial optical simulation software, FRED. Based on the simulated result, a film with an A-MLA was fabricated using the LIGA-like (Lithographie Galvanoformung Abformung, LIGA) process, including lithography, sputtering, micro-molding with PDMS (Polydimethylsiloxane) and UV (Ultraviolet)-cured technology. The major challenge to this process is to use JSR-126N negative thick photoresist to manufacture an approximate A-MLA optical film with multi-step lithography method. Finally, the films with A-MLAs are attached to an OLED to measure their optical-electric properties. The effectsof A-MLA optical films on OLED luminance are analyzed. In addition, the measured results are compared with simulated ones. They show good agreement with each other.

  17. The Design, Implementation, and Performance of the Astro-H SXS Calorimeter Array and Anti-Coincidence Detector

    Science.gov (United States)

    Kilbourne, Caroline A.; Adams, Joseph S.; Brekosky, Regis P.; Chiao, Meng P.; Chervenak, James A.; Eckart, Megan E.; Figueroa-Feliciano, Enectali; Galeazzi, Masimilliano; Grein, Christoph; Jhabvala, Christine A.; Kelley, Richard L.; Leutenegger, Maurice A.; McCammon, Dan; Porter, F. Scott; Szymkowiak, Andrew E.; Watanabe, Tomomi; Zhao, Jun

    2016-01-01

    The calorimeter array of the JAXA Astro-H (renamed Hitomi) Soft X-ray Spectrometer (SXS) was designed to provide unprecedented spectral resolution of spatially extended cosmic x-ray sources and of all cosmic x-ray sources in the Fe-K band around 6 keV, enabling essential plasma diagnostics. The SXS has a square array of 36 microcalorimeters at the focal plane. These calorimeters consist of ion-implanted silicon thermistors and HgTe thermalizing x-ray absorbers. These devices have demonstrated a resolution of better than 4.5 eV at 6 keV when operated at a heat-sink temperature of 50 mK. We will discuss the basic physical parameters of this array, including the array layout, thermal conductance of the link to the heat sink, resistance function, absorber details, and means of attaching the absorber to the thermistor-bearing element. We will also present the thermal characterization of the whole array, including thermal conductance and crosstalk measurements and the results of pulsing the frame temperature via alpha particles, heat pulses, and the environmental background. A silicon ionization detector is located behind the calorimeter array and serves to reject events due to cosmic rays. We will briefly describe this anti-coincidence detector and its performance.

  18. Assessment of High-Voltage Photovoltaic Technologies for the Design of a Direct Drive Hall Effect Thruster Solar Array

    Science.gov (United States)

    Mikellides, I. G.; Jongeward, G. A.; Schneider, T.; Carruth, M. R.; Peterson, T.; Kerslake, T. W.; Snyder, D.; Ferguson, D.; Hoskins, A.

    2004-01-01

    A three-year program to develop a Direct Drive Hall-Effect Thruster system (D2HET) begun in 2001 as part of the NASA Advanced Cross-Enterprise Technology Development initiative. The system, which is expected to reduce significantly the power processing, complexity, weight, and cost over conventional low-voltage systems, will employ solar arrays that operate at voltages higher than (or equal to) 300 V. The lessons learned from the development of the technology also promise to become a stepping-stone for the production of the next generation of power systems employing high voltage solar arrays. This paper summarizes the results from experiments conducted mainly at the NASA Marshal Space Flight Center with two main solar array technologies. The experiments focused on electron collection and arcing studies, when the solar cells operated at high voltages. The tests utilized small coupons representative of each solar array technology. A hollow cathode was used to emulate parts of the induced environment on the solar arrays, mostly the low-energy charge-exchange plasma (1012-1013 m-3 and 0.5-1 eV). Results and conclusions from modeling of electron collection are also summarized. The observations from the total effort are used to propose a preliminary, new solar array design for 2 kW and 30-40 kW class, deep space missions that may employ a single or a cluster of Hall- Effect thrusters.

  19. Genomic characterization of some Iranian children with idiopathic mental retardation using array comparative genomic hybridization

    Directory of Open Access Journals (Sweden)

    Farkhondeh Behjati

    2013-01-01

    Full Text Available Background: Mental retardation (MR has a prevalence of 1-3% and genetic causes are present in more than 50% of patients. Chromosomal abnormalities are one of the most common genetic causes of MR and are responsible for 4-28% of mental retardation. However, the smallest loss or gain of material visible by standard cytogenetic is about 4 Mb and for smaller abnormalities, molecular cytogenetic techniques such as array comparative genomic hybridization (array CGH should be used. It has been shown that 15-25% of idiopathic MR (IMR has submicroscopic rearrangements detectable by array CGH. In this project, the genomic abnormalities were investigated in 32 MR patients using this technique. Materials and Methods: Patients with IMR with dysmorphism were investigated in this study. Karyotype analysis, fragile X and metabolic tests were first carried out on the patients. The copy number variation was then assessed in a total of 32 patients with normal results for the mentioned tests using whole genome oligo array CGH. Multiple ligation probe amplification was carried out as a confirmation test. Results: In total, 19% of the patients showed genomic abnormalities. This is reduced to 12.5% once the two patients with abnormal karyotypes (upon re-evaluation are removed. Conclusion: The array CGH technique increased the detection rate of genomic imbalances in our patients by 12.5%. It is an accurate and reliable method for the determination of genomic imbalances in patients with IMR and dysmorphism.

  20. Genome-wide mapping of copy number variation in humans: comparative analysis of high resolution array platforms.

    Directory of Open Access Journals (Sweden)

    Rajini R Haraksingh

    Full Text Available Accurate and efficient genome-wide detection of copy number variants (CNVs is essential for understanding human genomic variation, genome-wide CNV association type studies, cytogenetics research and diagnostics, and independent validation of CNVs identified from sequencing based technologies. Numerous, array-based platforms for CNV detection exist utilizing array Comparative Genome Hybridization (aCGH, Single Nucleotide Polymorphism (SNP genotyping or both. We have quantitatively assessed the abilities of twelve leading genome-wide CNV detection platforms to accurately detect Gold Standard sets of CNVs in the genome of HapMap CEU sample NA12878, and found significant differences in performance. The technologies analyzed were the NimbleGen 4.2 M, 2.1 M and 3×720 K Whole Genome and CNV focused arrays, the Agilent 1×1 M CGH and High Resolution and 2×400 K CNV and SNP+CGH arrays, the Illumina Human Omni1Quad array and the Affymetrix SNP 6.0 array. The Gold Standards used were a 1000 Genomes Project sequencing-based set of 3997 validated CNVs and an ultra high-resolution aCGH-based set of 756 validated CNVs. We found that sensitivity, total number, size range and breakpoint resolution of CNV calls were highest for CNV focused arrays. Our results are important for cost effective CNV detection and validation for both basic and clinical applications.

  1. Design of Multilevel Sequential Rotation Feeding Networks Used for Circularly Polarized Microstrip Antenna Arrays

    Directory of Open Access Journals (Sweden)

    Aixin Chen

    2012-01-01

    Full Text Available Sequential rotation feeding networks can significantly improve performance of the circularly polarized microstrip antenna array. In this paper, single, double, and multiple series-parallel sequential rotation feeding networks are examined. Compared with conventional parallel feeding structures, these multilevel feeding techniques present reduction of loss, increase of bandwidth, and improvement of radiation pattern and polarization purity. By using corner-truncated square patch as the array element and adopting appropriate level of sequential rotation series-parallel feeding structures as feeding networks, microstrip arrays can generate excellent circular polarization (CP over a relatively wide frequency band. They can find wide applications in phased array radar and satellite communication systems.

  2. Research on analytical model and design formulas of permanent magnetic bearings based on Halbach array with arbitrary segmented magnetized angle

    Science.gov (United States)

    Wang, Nianxian; Wang, Dongxiong; Chen, Kuisheng; Wu, Huachun

    2016-07-01

    The bearing capacity of permanent magnetic bearings can be improved efficiently by using the Halbach array magnetization. However, the research on analytical model of Halbach array PMBs with arbitrary segmented magnetized angle has not been developed. The application of Halbach array PMBs has been limited by the absence of the analytical model and design formulas. In this research, the Halbach array PMBs with arbitrary segmented magnetized angle has been studied. The magnetization model of bearings is established. The magnetic field distribution model of the permanent magnet array is established by using the scalar magnetic potential model. On the basis of this, the bearing force model and the bearing stiffness model of the PMBs are established based on the virtual displacement method. The influence of the pair of magnetic rings in one cycle and the structure parameters of PMBs on the maximal bearing capacity and support stiffness characteristics are studied. The reference factors for the design process of PMBs have been given. Finally, the theoretical model and the conclusions are verified by the finite element analysis.

  3. Microdeletion and microduplication analysis of chinese conotruncal defects patients with targeted array comparative genomic hybridization.

    Directory of Open Access Journals (Sweden)

    Xiaohui Gong

    Full Text Available OBJECTIVE: The current study aimed to develop a reliable targeted array comparative genomic hybridization (aCGH to detect microdeletions and microduplications in congenital conotruncal defects (CTDs, especially on 22q11.2 region, and for some other chromosomal aberrations, such as 5p15-5p, 7q11.23 and 4p16.3. METHODS: Twenty-seven patients with CTDs, including 12 pulmonary atresia (PA, 10 double-outlet right ventricle (DORV, 3 transposition of great arteries (TGA, 1 tetralogy of Fallot (TOF and one ventricular septal defect (VSD, were enrolled in this study and screened for pathogenic copy number variations (CNVs, using Agilent 8 x 15K targeted aCGH. Real-time quantitative polymerase chain reaction (qPCR was performed to test the molecular results of targeted aCGH. RESULTS: Four of 27 patients (14.8% had 22q11.2 CNVs, 1 microdeletion and 3 microduplications. qPCR test confirmed the microdeletion and microduplication detected by the targeted aCGH. CONCLUSION: Chromosomal abnormalities were a well-known cause of multiple congenital anomalies (MCA. This aCGH using arrays with high-density coverage in the targeted regions can detect genomic imbalances including 22q11.2 and other 10 kinds CNVs effectively and quickly. This approach has the potential to be applied to detect aneuploidy and common microdeletion/microduplication syndromes on a single microarray.

  4. Design and fabrication of an elliptical micro-lens array with grating for laser safety

    Science.gov (United States)

    Li, L. H.; Wu, B. Q.; Chan, C. Y.; Lee, W. B.; Dong, L. H.

    2015-10-01

    With the enormous expansion of laser usage in medicine, industry and research, all facilities must formulate and adhere to specific safety methods that appropriately address user protection. The protective ellipticalal microstructure with grating is a novel technology which can provide the principal means of ensuring against ocular injury, and must be worn at all times during laser operation. On the basis of Fresnel's law and the diffractive law, Solidworks and Lighttools software are applied to design the elliptical micro-lens array and correspondent grating. The height of the microstructure is 100um and its period is 3mm. The period of grating is 5um. It is shown that the amount of emergent light of a specific wavelength (1064nm) can reflect more than 40° from the incident light through simulation, while the incident light is perpendicular to the microstructure. The fabrication adopts the ultra-precision single point diamond method and injection molding method. However, it is found in the test that the surface roughness has a serious effect on the angle between the emergent and incident light. As a result, the element can reflect the vertical incidence beam into a tilted emergent beam with a certain angular degree , as well as protecting users from laser damage injures.

  5. Acoustic anechoic layers with singly periodic array of scatterers: Computational methods, absorption mechanisms, and optimal design

    Science.gov (United States)

    Yang, Hai-Bin; Li, Yue; Zhao, Hong-Gang; Wen, Ji-Hong; Wen, Xi-Sen

    2014-10-01

    The acoustic properties of anechoic layers with a singly periodic array of cylindrical scatterers are investigated. A method combined plane wave expansion and finite element analysis is extended for out-of-plane incidence. The reflection characteristics of the anechoic layers with cavities and locally resonant scatterers are discussed. The backing is a steel plate followed by an air half space. Under this approximate zero transmission backing condition, the reflection reduction is induced by the absorption enhancement. The absorption mechanism is explained by the scattering/absorption cross section of the isolated scatterer. Three types of resonant modes which can induce efficient absorption are revealed. Due to the fact that the frequencies of the resonant modes are related to the size of the scatterers, anechoic layers with scatterers of mixed size can broaden the absorption band. A genetic optimization algorithm is adopted to design the anechoic layer with scatterers of mixed size at a desired frequency band from 2 kHz to 10 kHz for normal incidence, and the influence of the incident angle is also discussed.

  6. Modular design and implementation of field-programmable-gate-array-based Gaussian noise generator

    Science.gov (United States)

    Li, Yuan-Ping; Lee, Ta-Sung; Hwang, Jeng-Kuang

    2016-05-01

    The modular design of a Gaussian noise generator (GNG) based on field-programmable gate array (FPGA) technology was studied. A new range reduction architecture was included in a series of elementary function evaluation modules and was integrated into the GNG system. The approximation and quantisation errors for the square root module with a first polynomial approximation were high; therefore, we used the central limit theorem (CLT) to improve the noise quality. This resulted in an output rate of one sample per clock cycle. We subsequently applied Newton's method for the square root module, thus eliminating the need for the use of the CLT because applying the CLT resulted in an output rate of two samples per clock cycle (>200 million samples per second). Two statistical tests confirmed that our GNG is of high quality. Furthermore, the range reduction, which is used to solve a limited interval of the function approximation algorithms of the System Generator platform using Xilinx FPGAs, appeared to have a higher numerical accuracy, was operated at >350 MHz, and can be suitably applied for any function evaluation.

  7. Application-specific coarse-grained reconfigurable array: architecture and design methodology

    Science.gov (United States)

    Zhou, Li; Liu, Dongpei; Zhang, Jianfeng; Liu, Hengzhu

    2015-06-01

    Coarse-grained reconfigurable arrays (CGRAs) have shown potential for application in embedded systems in recent years. Numerous reconfigurable processing elements (PEs) in CGRAs provide flexibility while maintaining high performance by exploring different levels of parallelism. However, a difference remains between the CGRA and the application-specific integrated circuit (ASIC). Some application domains, such as software-defined radios (SDRs), require flexibility with performance demand increases. More effective CGRA architectures are expected to be developed. Customisation of a CGRA according to its application can improve performance and efficiency. This study proposes an application-specific CGRA architecture template composed of generic PEs (GPEs) and special PEs (SPEs). The hardware of the SPE can be customised to accelerate specific computational patterns. An automatic design methodology that includes pattern identification and application-specific function unit generation is also presented. A mapping algorithm based on ant colony optimisation is provided. Experimental results on the SDR target domain show that compared with other ordinary and application-specific reconfigurable architectures, the CGRA generated by the proposed method performs more efficiently for given applications.

  8. Design and Performance Evaluation of Two-Unit YAGI-UDA Array for UHF Satellite Communication

    Directory of Open Access Journals (Sweden)

    Rupesh Lad

    2014-11-01

    Full Text Available Cube satellite missions perform innovative scientif ic experiments on a low cost developmental platform but have an inherent limitation of size and space. This restricts the total available solar power that can be harnessed and as a result, the radio links operate on stringent power budgets. For improving the avail able margins for communication in such satellites, it is desirable to improve upon the antenna system performance at the ground station used for the esta blishment of the links with the satellite. This can be achieved by improving the forward gain, the forward to backward ratio and the directivity of the anten na. This paper describes the electrical simulations and the performance evaluation of the one unit, two un it and four unit circularly polarized crossed Yagi-Uda ant enna array designed for communication with amateur radio (HAM satellites operating over the 434 MHz t o 438 MHz Amateur UHF band. The electro-magnetic model has been developed using the 4NEC2 software. The simulations have been validated with the practical field testing performed for estimating th e SWR, antenna gain, the forward to backward ratio and radiation pattern for the antenna system

  9. Design and performance of acoustic planar array antenna for Doppler sodar

    Directory of Open Access Journals (Sweden)

    A.Kamalakumari

    2012-09-01

    Full Text Available The remote probing of the thermal structure and the wind velocity in the lower atmosphere requires highly directive antennas, which should have a half-power beam width of about ±10° (3-dB points, a 90°-sidelobe suppression of at least 40 dB, little volume reverberation, low wind sensitivity, and be made of weatherproofmaterial. In this paper, various off-axis planar antennas have been built and acoustically evaluated the design aspects, measured results and field patterns of an 8x8 acoustic planar array antennas are described. Tweeter elements are arranged in a planar geometry and are capable of transmitting vertical polarization when placed nearly horizontal, are appropriate for the sodar applications. The performance of 8x8 planar antennas are tested experimentally for the frequencies from 1.8KHz to 4KHz at elevation angles ranging between 0 to 180°.The experimental results, as well as the measured beam widths and field patterns of this antennas, are presented.

  10. Studie zur Häufigkeit und Art von Chromosomenanomalien unter frühen Spontanaborten ohne in vitro Wachstum - Retrospektive Untersuchung mittels Comparativer Genomhybridisierung (CGH)

    OpenAIRE

    Pircher, Marion

    2007-01-01

    Die CGH ist eine molekularzytogenetische Methode, welche chromosomale Imbalancen durch reverse Floureszenz-in-situ-Hybridisierung zuverlässig aufdecken kann. Dadurch dass ausschließlich genomische DNA und keine Metaphasen des zu untersuchenden Materials benötigt werden, umgeht die CGH damit die Einschränkungen der konventionellen Chromosomenanalyse. In dieser Studie wurde das diagnostische Potential der CGH zur retrospekt...

  11. Using a sensitivity study to facilitate the design of a multi-electrode array to measure six cardiac conductivity values.

    Science.gov (United States)

    Johnston, Barbara M

    2013-07-01

    When using the bidomain model to model the electrical activity of the heart, there are potentially six cardiac conductivity values involved: conductivity values in directions along and normal to the cardiac fibres with a sheet, as well as a conductivity value in the normal direction between the sheets, and these occur for both the extracellular and intracellular domains in the model. To date it has been common to assume that the two normal direction conductivity values are the same. However, recent work has demonstrated that six cardiac conductivity values, rather than four, are necessary for accurate modelling, which can then facilitate understanding of cardiovascular disease. To design a method to determine these conductivities, it is also necessary to design a suitable multi-electrode array, which can be used, in conjunction with an inversion technique, to retrieve conductivity values from measurements of potential made on the array. This work uses the results of a study, into the sensitivity of the measuring potentials to variability in the input conductivities, to facilitate the design of an array that could be used to retrieve six cardiac conductivity values, as well as fibre rotation angle. It is found that if an electrode in the array has a much lower value of potential than the other electrodes, then it tends to be much more sensitive to the input conductivities than the other electrodes. It also appears that inclusion of this type of electrode in the set of measuring electrodes is essential for accurately retrieving conductivity values. This technique is used to identify electrodes to be included in the array and using the final design it is demonstrated, using synthetic values of potential, that the six cardiac conductivity values, and the fibre rotation angle, can be retrieved very accurately.

  12. Compression and radiation of high-power short rf pulses. II. A novel antenna array design with combined compressor/radiator elements

    KAUST Repository

    Sirenko, Kostyantyn

    2011-01-01

    The paper discusses the radiation of compressed high power short RF pulses using two different types of antennas: (i) A simple monopole antenna and (ii) a novel array design, where each of the elements is constructed by combining a compressor and a radiator. The studies on the monopole antenna demonstrate the possibility of a high power short RF pulse\\'s efficient radiation even using simple antennas. The studies on the novel array design demonstrate that a reduced size array with lower pulse distortion and power decay can be constructed by assembling the array from elements each of which integrates a compressor and a radiator. This design idea can be used with any type of antenna array; in this work it is applied to a phased array.

  13. Solar Powered Aircraft, Photovoltaic Array/Battery System Tabletop Demonstration: Design and Operation Manual

    Science.gov (United States)

    Colozza, Anthony J.; Scheiman, David A.; Bailey, Sheila (Technical Monitor)

    2000-01-01

    A system was constructed to demonstrate the power system operation of a solar powered aircraft. The system consists of a photovoltaic (PV) array, a charge controller, a battery, an electric motor and propeller. The system collects energy from the PV array and either utilizes this energy to operate an electric motor or stores it in a rechargeable battery for future use. The system has a control panel which displays the output of the array and battery as well as the total current going to the electric motor. The control panel also has a means for adjusting the output to the motor to control its speed. The entire system is regulated around 12 VDC.

  14. Advanced design of conductive polymeric arrays with controlled electrical resistance using direct laser interference patterning

    Energy Technology Data Exchange (ETDEWEB)

    Lasagni, A.F. [Saarland University, Department of Materials Science, Chair of Functional Materials, Building C 6.3, 7. Stock, P.O. Box 15 11 50, Saarbruecken (Germany); George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA (United States); Acevedo, D.F. [Saarland University, Department of Materials Science, Chair of Functional Materials, Building C 6.3, 7. Stock, P.O. Box 15 11 50, Saarbruecken (Germany); Universidad Nacional de Rio Cuarto, Departamento de Quimica, Cordoba (Argentina); Barbero, C.A. [Universidad Nacional de Rio Cuarto, Departamento de Quimica, Cordoba (Argentina); Muecklich, F. [Saarland University, Department of Materials Science, Chair of Functional Materials, Building C 6.3, 7. Stock, P.O. Box 15 11 50, Saarbruecken (Germany)

    2008-06-15

    In this work, we report a simple method for the fabrication of regular conducting polyaniline periodic arrays on large areas of glass or gold substrates using direct laser interference patterning. Additionally, by controlling the laser intensity it is possible to precisely tune the width of the periodic arrays and consequently the electrical resistance of the polyaniline strips. The periodic arrays were characterized using scanning electron microscopy, white light interferometry and cyclic voltametry. The great importance of the method reported lies both in its versatility and the ability to control the properties of the modified polymer electrodes with high precision. This is important for prospective applications such as electrochemical sensors. (orig.)

  15. Optimal Design of Low-Density SNP Arrays for Genomic Prediction: Algorithm and Applications.

    Science.gov (United States)

    Wu, Xiao-Lin; Xu, Jiaqi; Feng, Guofei; Wiggans, George R; Taylor, Jeremy F; He, Jun; Qian, Changsong; Qiu, Jiansheng; Simpson, Barry; Walker, Jeremy; Bauck, Stewart

    2016-01-01

    Low-density (LD) single nucleotide polymorphism (SNP) arrays provide a cost-effective solution for genomic prediction and selection, but algorithms and computational tools are needed for the optimal design of LD SNP chips. A multiple-objective, local optimization (MOLO) algorithm was developed for design of optimal LD SNP chips that can be imputed accurately to medium-density (MD) or high-density (HD) SNP genotypes for genomic prediction. The objective function facilitates maximization of non-gap map length and system information for the SNP chip, and the latter is computed either as locus-averaged (LASE) or haplotype-averaged Shannon entropy (HASE) and adjusted for uniformity of the SNP distribution. HASE performed better than LASE with ≤1,000 SNPs, but required considerably more computing time. Nevertheless, the differences diminished when >5,000 SNPs were selected. Optimization was accomplished conditionally on the presence of SNPs that were obligated to each chromosome. The frame location of SNPs on a chip can be either uniform (evenly spaced) or non-uniform. For the latter design, a tunable empirical Beta distribution was used to guide location distribution of frame SNPs such that both ends of each chromosome were enriched with SNPs. The SNP distribution on each chromosome was finalized through the objective function that was locally and empirically maximized. This MOLO algorithm was capable of selecting a set of approximately evenly-spaced and highly-informative SNPs, which in turn led to increased imputation accuracy compared with selection solely of evenly-spaced SNPs. Imputation accuracy increased with LD chip size, and imputation error rate was extremely low for chips with ≥3,000 SNPs. Assuming that genotyping or imputation error occurs at random, imputation error rate can be viewed as the upper limit for genomic prediction error. Our results show that about 25% of imputation error rate was propagated to genomic prediction in an Angus population. The

  16. Comparison of chromosomal and array-based comparative genomic hybridization for the detection of genomic imbalances in primary prostate carcinomas

    Directory of Open Access Journals (Sweden)

    Berg Marianne

    2006-09-01

    Full Text Available Abstract Background In order to gain new insights into the molecular mechanisms involved in prostate cancer, we performed array-based comparative genomic hybridization (aCGH on a series of 46 primary prostate carcinomas using a 1 Mbp whole-genome coverage platform. As chromosomal comparative genomic hybridization (cCGH data was available for these samples, we compared the sensitivity and overall concordance of the two methodologies, and used the combined information to infer the best of three different aCGH scoring approaches. Results Our data demonstrate that the reliability of aCGH in the analysis of primary prostate carcinomas depends to some extent on the scoring approach used, with the breakpoint estimation method being the most sensitive and reliable. The pattern of copy number changes detected by aCGH was concordant with that of cCGH, but the higher resolution technique detected 2.7 times more aberrations and 15.2% more carcinomas with genomic imbalances. We additionally show that several aberrations were consistently overlooked using cCGH, such as small deletions at 5q, 6q, 12p, and 17p. The latter were validated by fluorescence in situ hybridization targeting TP53, although only one carcinoma harbored a point mutation in this gene. Strikingly, homozygous deletions at 10q23.31, encompassing the PTEN locus, were seen in 58% of the cases with 10q loss. Conclusion We conclude that aCGH can significantly improve the detection of genomic aberrations in cancer cells as compared to previously established whole-genome methodologies, although contamination with normal cells may influence the sensitivity and specificity of some scoring approaches. Our work delineated recurrent copy number changes and revealed novel amplified loci and frequent homozygous deletions in primary prostate carcinomas, which may guide future work aimed at identifying the relevant target genes. In particular, biallelic loss seems to be a frequent mechanism of inactivation

  17. Quadrature transmit array design using single-feed circularly polarized patch antenna for parallel transmission in MR imaging.

    Science.gov (United States)

    Pang, Yong; Yu, Baiying; Vigneron, Daniel B; Zhang, Xiaoliang

    2014-02-01

    Quadrature coils are often desired in MR applications because they can improve MR sensitivity and also reduce excitation power. In this work, we propose, for the first time, a quadrature array design strategy for parallel transmission at 298 MHz using single-feed circularly polarized (CP) patch antenna technique. Each array element is a nearly square ring microstrip antenna and is fed at a point on the diagonal of the antenna to generate quadrature magnetic fields. Compared with conventional quadrature coils, the single-feed structure is much simple and compact, making the quadrature coil array design practical. Numerical simulations demonstrate that the decoupling between elements is better than -35 dB for all the elements and the RF fields are homogeneous with deep penetration and quadrature behavior in the area of interest. Bloch equation simulation is also performed to simulate the excitation procedure by using an 8-element quadrature planar patch array to demonstrate its feasibility in parallel transmission at the ultrahigh field of 7 Tesla. PMID:24649430

  18. Design a New Strategy Based on Nanoparticle-Enhanced Chemiluminescence Sensor Array for Biothiols Discrimination.

    Science.gov (United States)

    Shahrajabian, Maryam; Hormozi-Nezhad, M Reza

    2016-01-01

    Array-based sensor is an interesting approach that suggests an alternative to expensive analytical methods. In this work, we introduce a novel, simple, and sensitive nanoparticle-based chemiluminescence (CL) sensor array for discrimination of biothiols (e.g., cysteine, glutathione and glutathione disulfide). The proposed CL sensor array is based on the CL efficiencies of four types of enhanced nanoparticle-based CL systems. The intensity of CL was altered to varying degrees upon interaction with biothiols, producing unique CL response patterns. These distinct CL response patterns were collected as "fingerprints" and were then identified through chemometric methods, including linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA). The developed array was able to successfully differentiate between cysteine, glutathione and glutathione disulfide in a wide concentration range. Moreover, it was applied to distinguish among the above analytes in human plasma. PMID:27574247

  19. Design a New Strategy Based on Nanoparticle-Enhanced Chemiluminescence Sensor Array for Biothiols Discrimination

    Science.gov (United States)

    Shahrajabian, Maryam; Hormozi-Nezhad, M. Reza

    2016-08-01

    Array-based sensor is an interesting approach that suggests an alternative to expensive analytical methods. In this work, we introduce a novel, simple, and sensitive nanoparticle-based chemiluminescence (CL) sensor array for discrimination of biothiols (e.g., cysteine, glutathione and glutathione disulfide). The proposed CL sensor array is based on the CL efficiencies of four types of enhanced nanoparticle-based CL systems. The intensity of CL was altered to varying degrees upon interaction with biothiols, producing unique CL response patterns. These distinct CL response patterns were collected as “fingerprints” and were then identified through chemometric methods, including linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA). The developed array was able to successfully differentiate between cysteine, glutathione and glutathione disulfide in a wide concentration range. Moreover, it was applied to distinguish among the above analytes in human plasma.

  20. Design a New Strategy Based on Nanoparticle-Enhanced Chemiluminescence Sensor Array for Biothiols Discrimination

    Science.gov (United States)

    Shahrajabian, Maryam; Hormozi-Nezhad, M. Reza

    2016-01-01

    Array-based sensor is an interesting approach that suggests an alternative to expensive analytical methods. In this work, we introduce a novel, simple, and sensitive nanoparticle-based chemiluminescence (CL) sensor array for discrimination of biothiols (e.g., cysteine, glutathione and glutathione disulfide). The proposed CL sensor array is based on the CL efficiencies of four types of enhanced nanoparticle-based CL systems. The intensity of CL was altered to varying degrees upon interaction with biothiols, producing unique CL response patterns. These distinct CL response patterns were collected as “fingerprints” and were then identified through chemometric methods, including linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA). The developed array was able to successfully differentiate between cysteine, glutathione and glutathione disulfide in a wide concentration range. Moreover, it was applied to distinguish among the above analytes in human plasma. PMID:27574247

  1. Design of optical element combining Fresnel lens with microlens array for uniform light-emitting diode lighting.

    Science.gov (United States)

    Wang, Guangzhen; Wang, Lili; Li, Fuli; Kong, Depeng

    2012-09-01

    One kind of optical element combining Fresnel lens with microlens array is designed simply for LED lighting based on geometrical optics and nonimaging optics. This design method imposes no restriction on the source intensity pattern. The designed element has compact construction and can produce multiple shapes of illumination distribution. Taking square lighting as an example, tolerance analysis is carried out to determine tolerance limits for applying the element in the assembly process. This element can produce on-axis lighting and off-axis lighting. PMID:23201944

  2. Design of Multilevel Sequential Rotation Feeding Networks Used for Circularly Polarized Microstrip Antenna Arrays

    OpenAIRE

    Aixin Chen; Chuo Yang; Zhizhang (David) Chen; Yanjun Zhang; Yingyi He

    2012-01-01

    Sequential rotation feeding networks can significantly improve performance of the circularly polarized microstrip antenna array. In this paper, single, double, and multiple series-parallel sequential rotation feeding networks are examined. Compared with conventional parallel feeding structures, these multilevel feeding techniques present reduction of loss, increase of bandwidth, and improvement of radiation pattern and polarization purity. By using corner-truncated square patch as the array e...

  3. Clone-array pooled shotgun mapping and sequencing: design and analysis of experiments

    OpenAIRE

    Csürös, Miklós; Li, Bingshan; Milosavljevic, Aleksandar

    2003-01-01

    This paper studies sequencing and mapping methods that rely solely on pooling and shotgun sequencing of clones. First, we scrutinize and improve the recently proposed Clone-Array Pooled Shotgun Sequencing (CAPSS) method, which delivers a BAC-linked assembly of a whole genome sequence. Secondly, we introduce a novel physical mapping method, called Clone-Array Pooled Shotgun Mapping (CAPS-MAP), which computes the physical ordering of BACs in a random library. Both CAPSS and CAPS-MAP construct s...

  4. Design and implementation of Gm-APD array readout integrated circuit for infrared 3D imaging

    Science.gov (United States)

    Zheng, Li-xia; Yang, Jun-hao; Liu, Zhao; Dong, Huai-peng; Wu, Jin; Sun, Wei-feng

    2013-09-01

    A single-photon detecting array of readout integrated circuit (ROIC) capable of infrared 3D imaging by photon detection and time-of-flight measurement is presented in this paper. The InGaAs avalanche photon diodes (APD) dynamic biased under Geiger operation mode by gate controlled active quenching circuit (AQC) are used here. The time-of-flight is accurately measured by a high accurate time-to-digital converter (TDC) integrated in the ROIC. For 3D imaging, frame rate controlling technique is utilized to the pixel's detection, so that the APD related to each pixel should be controlled by individual AQC to sense and quench the avalanche current, providing a digital CMOS-compatible voltage pulse. After each first sense, the detector is reset to wait for next frame operation. We employ counters of a two-segmental coarse-fine architecture, where the coarse conversion is achieved by a 10-bit pseudo-random linear feedback shift register (LFSR) in each pixel and a 3-bit fine conversion is realized by a ring delay line shared by all pixels. The reference clock driving the LFSR counter can be generated within the ring delay line Oscillator or provided by an external clock source. The circuit is designed and implemented by CSMC 0.5μm standard CMOS technology and the total chip area is around 2mm×2mm for 8×8 format ROIC with 150μm pixel pitch. The simulation results indicate that the relative time resolution of the proposed ROIC can achieve less than 1ns, and the preliminary test results show that the circuit function is correct.

  5. High-Frequency Ultrasound Array Designed for Ultrasound-Guided Breast Biopsy.

    Science.gov (United States)

    Cummins, Thomas; Eliahoo, Payam; Kirk Shung, K

    2016-06-01

    This paper describes the development of a miniaturized high-frequency linear array that can be integrated within a core biopsy needle to improve tissue sampling accuracy during breast cancer biopsy procedures. The 64-element linear array has an element width of [Formula: see text], kerf width of [Formula: see text], element length of 1 mm, and element thickness of [Formula: see text]. The 2-2 array composite was fabricated using deep reactive ion etching of lead magnesium niobate-lead titanate (PMN-PT) single crystal material. The array composite fabrication process as well as a novel high-density electrical interconnect solution are presented and discussed. Array performance measurements show that the array had a center frequency and fractional bandwidth ([Formula: see text]) of 59.1 MHz and 29.4%, respectively. Insertion loss and adjacent element crosstalk at the center frequency were -41.0 and [Formula: see text], respectively. A B-mode image of a tungsten wire target phantom was captured using a synthetic aperture imaging system and the imaging test results demonstrate axial and lateral resolutions of 33.2 and [Formula: see text], respectively. PMID:27046895

  6. Design and Fabrication of a Two-Dimensional Superconducting Pop-up Bolometer Array

    Science.gov (United States)

    Benford, Dominic J.; Staguhn, Johannes G.; Chervenak, James A.; Allen, Christine A.; Moseley, S. Harvey; Irwin, Kent D.; Stacey, Gordon J.; Page, Lyman A.

    2004-01-01

    We have been developing an architecture for producing large format, two dimensional arrays of close-packed bolometers, which will enable submillimeter cameras and spectrometers to obtain images and spectra orders of magnitude faster than present instruments. The low backgrounds achieved in these instruments require very sensitive detectors with NEPs of order 5 x 10(exp -18) W/square root of Hz. Superconducting transition edge sensor bolometers can be close-packed using the Pop-up Detector (PUD) format, and SQUID multiplexers operating at the detector base temperature can be intimately coupled to them. The array unit cell is 8 x 32 pixels, using 32- element detector and multiplexer components. We have fabricated an engineering model array with this technology which features a very compact, modular approach for large format arrays. We report on the production of the 32-element components for the arrays. Planned instruments using this array architecture include the Submillimeter and Far-InfraRed Experiment (SAFIRE) on the SOFIA airborne observatory, the South Pole Imaging Fabry-Perot Interferometer (SPIFI) for the AST/RO observatory, the Millimeter Bolometer Camera for the Atacama Cosmology Telescope (MBC/ACT), and the Redshift (Z) Early Universe Spectrometer (ZEUS j.

  7. A sensitivity analysis for the F100 turbofan engine using the multivariable Nyquist array. [feedback control design

    Science.gov (United States)

    Leininger, G. G.; Borysiak, M. L.

    1978-01-01

    In the feedback control design of multivariable systems, closed loop performance evaluations must include the dynamic behavior of variables unavailable to the feedback controller. For the multivariable Nyquist array method, a set of sensitivity functions are proposed to simplify the adjustment of compensator parameters when the dynamic response of the unmeasurable output variables is unacceptable. A sensitivity study to improve thrust and turbine temperature responses for the Pratt-Whitney F100 turbofan engine demonstrates the utility of the proposed method.

  8. Recent advances in image reconstruction, coil sensitivity calibration, and coil array design for SMASH and generalized parallel MRI.

    Science.gov (United States)

    Sodickson, Daniel K; McKenzie, Charles A; Ohliger, Michael A; Yeh, Ernest N; Price, Mark D

    2002-01-01

    Parallel magnetic resonance imaging (MRI) techniques use spatial information from arrays of radiofrequency (RF) detector coils to accelerate imaging. A number of parallel MRI techniques have been described in recent years, and numerous clinical applications are currently being explored. The advent of practical parallel imaging presents various challenges for image reconstruction and RF system design. Recent advances in tailored SiMultaneous Acquisition of Spatial Harmonics (SMASH) image reconstructions are summarized. These advances enable robust SMASH imaging in arbitrary image planes with a wide range of coil array geometries. A generalized formalism is described which may be used to understand the relations between SMASH and SENSE, to derive typical implementations of each as special cases, and to form hybrid techniques combining some of the advantages of both. Accurate knowledge of coil sensitivities is crucial for parallel MRI, and errors in calibration represent one of the most common and the most pernicious sources of error in parallel image reconstructions. As one example, motion of the patient and/or the coil array between the sensitivity reference scan and the accelerated acquisition can lead to calibration errors and reconstruction artifacts. Self-calibrating parallel MRI approaches that address this problem by eliminating the need for external sensitivity references are reviewed. The ultimate achievable signal-to-noise ratio (SNR) for parallel MRI studies is closely tied to the geometry and sensitivity patterns of the coil arrays used for spatial encoding. Several parallel imaging array designs that depart from the traditional model of overlapped adjacent loop elements are described. PMID:11755091

  9. Design and development of compact readout electronics with silicon photomultiplier array for a compact imaging detector

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xiao-Hui; QI Yu-Jin; ZHAO Cui-Lan

    2012-01-01

    This work aims at developing compact readout electronics for a compact imaging detector module with silicon photomultiplier (SPM) array.The detector module consists of a LYSO crystal array coupling with a SensL's 4×4 SPM array.A compact multiplexed readout based on a discretized positioning circuit (DPC) was developed to reduce the readout channels from 16 to 4 outputs.Different LYSO crystal arrays of 4×4,8×8 and 12×12 with pixel sizes of 3.2,1.6 and 1.0 mm respectively,have been tested with the compact readout board using a 137Cs source.The initial results show that the compact imaging detector module with the compact multiplexed readout could clearly resolve 1 mm×1 mm×10 mm LYSO scintillation crystal array except those at the edges.The detector's intrinsic spatial resolution up to 1 mm can be achieved with the 3 mm×3 mm size SPMArray4 through light sharing and compact multiplexed readout.Our results indicate that this detector module is feasible for the development of high-resolution compact PET.

  10. Design Low Crosstalk Ring-Slot Array Structure for Label-Free Multiplexed Sensing

    Directory of Open Access Journals (Sweden)

    Lijun Huang

    2014-08-01

    Full Text Available We theoretically demonstrate a low crosstalk ring-slot array structure used for label-free multiplexed sensing. The proposed sensors array is based on an array of three ring-slot and input/output line defect coupling waveguides. Each ring-slot cavity has slightly different cavity spacing and different resonant frequency. Results obtained using two dimensional finite-difference time-domain (2D-FDTD simulation indicate that the resonant frequencies of each sensor unit in response to the refractive index variations are independent. The refractive index sensitivity is 134 ~ 145.5 nm/RIU (refractive index unit and the Q factors more than 104 can be achieved. The calculated detect limit lower than 1.13 × 10−4 RIU is obtained. In addition, an extremely small crosstalk lower than −25.8 dB is achieved among the array of three ring-slot cavities. The results demonstrate that this multiplexed sensor array is a promising platform for integrated optical devices and enables highly parallel label-free detection.

  11. Genomic imbalances in 5918 malignant epithelial tumors: an explorative meta-analysis of chromosomal CGH data

    International Nuclear Information System (INIS)

    Chromosomal abnormalities have been associated with most human malignancies, with gains and losses on some genomic regions associated with particular entities. Of the 15429 cases collected for the Progenetix molecular-cytogenetic database, 5918 malignant epithelial neoplasias analyzed by chromosomal Comparative Genomic Hybridization (CGH) were selected for further evaluation. For the 22 clinico-pathological entities with more than 50 cases, summary profiles for genomic imbalances were generated from case specific data and analyzed. With large variation in overall genomic instability, recurring genomic gains and losses were prominent. Most entities showed frequent gains involving 8q2, while gains on 20q, 1q, 3q, 5p, 7q and 17q were frequent in different entities. Loss 'hot spots' included 3p, 4q, 13q, 17p and 18q among others. Related average imbalance patterns were found for clinically distinct entities, e.g. hepatocellular carcinomas (ca.) and ductal breast ca., as well as for histologically related entities (squamous cell ca. of different sites). Although considerable case-by-case variation of genomic profiles can be found by CGH in epithelial malignancies, a limited set of variously combined chromosomal imbalances may be typical for carcinogenesis. Focus on the respective regions should aid in target gene detection and pathway deduction

  12. Designing Adaptive Linear Array Antenna to Achieve Pattern Steering Optimization by Phase-Amplitude Perturbations Using Genetic Algorithms

    Institute of Scientific and Technical Information of China (English)

    HSUChaohsing; CHENTsongyi; PanJengshyang

    2005-01-01

    In this paper, a phase-amplitude perturbation method of an adaptive array factor based on the genetic algorithm is proposed. The design for an optimal beam pattern of an adaptive antenna is able to not only suppress interference by placing nulls at the directions of the interfering sources but also provide a maximized main lobe in the direction of the desired signal, i.e., to maximizethe Signal interference ratio (SIR). In order to achieve this goal, a kind of new convergent skill called the two-way convergent method for genetic algorithms is proposed. The phase-amplitude perturbation method is applied to realize the optimal beam pattern of an adaptively linear array antenna. The Genetic algorithms are applied to find the optimal phase-amplitude weighting vector of adaptive array factor. An optimal beam pattern of linear array is derived by phase-amplitude perturbations using a genetic algorithm. Computer simulation result is given to demonstrate the effectiveness of the proposed method.

  13. Radio Frequency Optics Design of the 12-Meter Antenna for the Array-Based Deep Space Network

    Science.gov (United States)

    Imbriale, W. A.

    2005-02-01

    Development of very large arrays of small antennas has been proposed as a way to increase the downlink capability of the NASA Deep Space Network (DSN) by two or three orders of magnitude, thereby enabling greatly increased science data from currently configured missions or enabling new mission concepts. The current concept is for an array of 400 x 12-meter antennas at each of three longitudes. The DSN array will utilize radio astronomy sources for phase calibration and will have wide bandwidth correlation processing for this purpose. JPL currently is building a 3-element interferometer composed of 6-meter antennas to prove the performance and cost of the DSN array. This article describes the radio frequency (RF) design of the 12-meter reflector that will use the same feed and electronics as the 6-meter antenna. The 6-meter antenna utilized Gregorian optics to enable tests with a low-frequency prime focus feed without removing the subreflector. However, for the 12-meter antenna, maximum gain divided by noise temperature (G/T) is the overriding requirement, and a trade-off study demonstrated that Cassegrain optics is far superior to Gregorian optics for maximum G/T. Hence, the 12-meter antenna utilizes Cassegrain optics.

  14. Diffraction aperture non-ideal behaviour of air coupled transducers array elements designed for NDT.

    Science.gov (United States)

    Prego Borges, J L; Montero de Espinosa, F; Salazar, J; Garcia-Alvarez, J; Chávez, J A; Turó, A; Garcia-Hernandez, M J

    2006-12-22

    Air coupled piezoelectric ultrasonic array transducers are a novel tool that could lead to interesting advances in the area of non-contact laminar material testing using Lamb wave's propagation techniques. A key issue on the development of such transducers is their efficient coupling to air media (impedance mismatch between the piezoelectric material and air is 90 dB or more). Adaptation layers are used in order to attain good matching and avoid possible serious signal degradation. However, the introduction of these matching layers modify the transducer surface behaviour and, consequently, radiation characteristics are altered, making the usual idealization criteria (of uniform surface movement) adopted for field simulation purposes inaccurate. In our system, we have a concave linear-array transducer of 64 elements (electrically coupled by pairs) working at 0.8 MHz made of PZ27 rectangular piezoceramics (15 mm x 0.3 mm) with two matching layers made of polyurethane and porous cellulose bonded on them. Experimental measurements of the acoustic aperture of single excited array elements have shown an increment on the geometrical dimensions of its active surface. A sub-millimeter vibrometer laser scan has revealed an extension of the aperture beyond the supposed physical single array element dimensions. Non-uniform symmetric apodized velocity surface vibration amplitude profile with a concave delay contour indicates the presumed existence of travelling wave phenomena over the surface of the outer array matching layer. Also, asymptotic propagation velocities around 2500 m/s and attenuation coefficient between 15 and 20 dB/mm has been determined for the travelling waves showing clear tendencies. Further comparisons between the experimental measurements of single array element field radiation diagram and simulated equivalent aperture counterpart reveal good agreement versus the ideal (uniform displaced) rectangular aperture. For this purpose an Impulse Response Method

  15. Planar array stack design aided by rapid prototyping in development of air-breathing PEMFC

    Science.gov (United States)

    Chen, Chen-Yu; Lai, Wei-Hsiang; Weng, Biing-Jyh; Chuang, Huey-Jan; Hsieh, Ching-Yuan; Kung, Chien-Chih

    The polymer electrolyte membrane fuel cell (PEMFC) is one of the most important research topics in the new and clean energy area. The middle or high power PEMFCs can be applied to the transportation or the distributed power system. But for the small power application, it is needed to match the power requirement of the product generally. On the other hand, the direct methanol fuel cell (DMFC) is one of the most common type that researchers are interested in, but recently the miniature or the micro-PEMFCs attract more attention due to their advantages of high open circuit voltage and high power density. The objective of this study is to develop a new air-breathing planar array fuel cell stacked from 10 cells made by rapid prototyping technology which has potential for fast commercial design, low cost manufacturing, and even without converters/inverters for the system. In this paper, the main material of flow field plates is acrylonitrile-butadiene-styrene (ABS) which allows the fuel cell be mass-manufactured by plastic injection molding technology. The rapid prototyping technology is applied to construct the prototype and verify the practicability of the proposed stack design. A 10-cell air-breathing miniature PEMFC stack with a volume of 6 cm × 6 cm × 0.9 cm is developed and tested. Its segmented membrane electrode assembly (MEA) is designed with the active surface area of 1.3 cm × 1.3 cm in each individual MEA. The platinum loading at anode and cathode are 0.2 mg cm -2 and 0.4 mg cm -2, respectively. Results show that the peak power densities of the parallel connected and serial connected stack are 99 mW cm -2 at 0.425 V and 92 mW cm -2 at 4.25 V, respectively under the conditions of 70 °C relative saturated humidity (i.e., dew point temperature), ambient temperature and free convection air. Besides, the stack performance is increased under forced convection. If the cell surface air is blown by an electric fan, the peak power densities of parallel connected and

  16. HDL Design for 1 Zetta Bits Per Second (1 Zbps Multichannel 64:1 LVDS Data Serializer & De-Serializer ASIC Array Card Design for 6th Sense and Future Ultra High Wireless and Mobile Communication Protocol Cards

    Directory of Open Access Journals (Sweden)

    P. N. V. M SASTRY

    2015-01-01

    Full Text Available The Aim is to HDL Design & Implementation for Exa Bit Rate Multichannel 64:1 LVDS Data Serializer & De-Serializer ASIC Array Card for Ultra High Speed Wireless Communication Products like Network On Chip Routers, Data Bus Communication Interface Applications, Cloud Computing Networks , Zetta bit Ethernet at Zetta Bit Rate Of Data Transfer Speed. Basically This Serializer Array Converts 64 bit parallel Data Array in to Serial Array Form on Transmitter Side and Transmission Done through High Speed Wireless Serial Communication Link and also Converts this Same Serial Array Data into Parallel Data Array on the Receiver Side by De-Serializer Array ASIC without any noise, also measure Very High Compressed Jitter Tolerance & Eye Diagram, Bit Error Rate through Analyzer. This LVDS Data SER-De-SER mainly used in High Speed Bus Communication Protocol Transceivers, Interface FPGA Add On Cards. The Process Of Design is Implemented through Verilog HDL / VHDL, Programming & Debugging Done Latest FPGA Board.

  17. Design and performance of a low noise, 128-channel ASIC preamplifier for readout of active matrix flat-panel imaging arrays

    CERN Document Server

    Maolinbay, M; Yarema, R J; Antonuk, L E; El-Mohri, Y; Yeakey, M

    2002-01-01

    Design architecture and performance measurements of a low noise, 128-channel application-specific-integrated-circuit (ASIC) preamplifier are reported. The ASIC was designed for readout of active matrix flat-panel imager (AMFPI) arrays. Such arrays, which presently can be made as large as 41 cmx41 cm and with pixel-to-pixel pitches down to approx 70 mu m, require large numbers of low noise, high density, custom integrated readout circuits. The design of this new chip is specifically tailored for research and development of active matrix flat-panel arrays for various medical imaging applications. The design architecture includes the following features: (1) Programmable signal gain which allows acquisition of a wide range of signal sizes from various array designs so as to optimize the signal-to-noise ratio; (2) Correlated double sampling (CDS) which significantly reduces certain noise components; (3) Pipelined readout (simultaneously sampling and multiplexing signals) which reduces image acquisition time; (4) P...

  18. Conceptual design of the early implementation of the NEutron Detector Array (NEDA) with AGATA

    International Nuclear Information System (INIS)

    The NEutron Detector Array (NEDA) project aims at the construction of a new high-efficiency compact neutron detector array to be coupled with large γ -ray arrays such as AGATA. The application of NEDA ranges from its use as selective neutron multiplicity filter for fusion-evaporation reaction to a large solid angle neutron tagging device. In the present work, possible configurations for the NEDA coupled with the Neutron Wall for the early implementation with AGATA has been simulated, using Monte Carlo techniques, in order to evaluate their performance figures. The goal of this early NEDA implementation is to improve, with respect to previous instruments, efficiency and capability to select multiplicity for fusion-evaporation reaction channels in which 1, 2 or 3 neutrons are emitted. Each NEDA detector unit has the shape of a regular hexagonal prism with a volume of about 3.23l and it is filled with the EJ301 liquid scintillator, that presents good neutron- γ discrimination properties. The simulations have been performed using a fusion-evaporation event generator that has been validated with a set of experimental data obtained in the 58Ni + 56Fe reaction measured with the Neutron Wall detector array. (orig.)

  19. Thermal design issues and performance of microcalorimeter arrays at sub-Kelvin temperatures

    NARCIS (Netherlands)

    Ridder, M.L.; Bruijn, M.P.; Hoevers, H.F.C.; Germeau, A.; Baars, N.H.R.; Krouwer, E.; Baar, van J.J.J.; Wiegerink, R.J.

    2003-01-01

    We have produced 5/spl times/5 pixel arrays of microcalorimeters using bulk micromachining. Analysis of our data provides the thermal conductivity parameters of Si/sub x/N/sub y/ 1 /spl mu/m thick membranes at 100 mK. Moreover we find that the thermal transport at 100 mK in Si beams, with dimensions

  20. Design of a Novel Experimental Facility for Testing of Tidal Arrays

    Directory of Open Access Journals (Sweden)

    Matevz Pintar

    2013-08-01

    Full Text Available In order to obtain the maximum amount of energy from tidal stream extraction devices, deployment in large arrays should be studied. The area of seabed with favorable conditions is fairly limited; therefore layout spacing has to be optimized. In this paper a feasibility study for a novel experimental facility, suitable for the testing of an array of tidal devices, is presented. To avoid space and scale limitations of towing tanks, testing is proposed to be performed in large lakes or calm seas using a self-propelled vessel, which will carry an array of devices with variable spacing, creating relevant speed differences and measuring their performance and loading. Using hydrodynamic scaling laws, an appropriate size for test turbines and the range of vessel speed was determined to fulfill experimental requirements. Computational fluid dynamic simulations, using the actuator disc method, have suggested a suitable turbine array configuration to resemble real application conditions. A simplified model of the vessel was analyzed using the finite elements method to determine the main scantlings. The hull resistance calculated by empirical formulae was found to be negligible compared to the resistance of the tested turbine. It was confirmed that turbine size and speed determined by scaling laws are also reasonable from a propulsion point of view.

  1. Conceptual design of the early implementation of the NEutron Detector Array (NEDA) with AGATA

    Energy Technology Data Exchange (ETDEWEB)

    Hueyuek, Tayfun; Gadea, Andres; Domingo-Pardo, Cesar [Universidad de Valencia, Instituto de Fisica Corpuscular, CSIC, Paterna (Valencia) (Spain); Di Nitto, Antonio [Istituto Nazionale di Fisica Nucleare, Napoli (Italy); Johannes Gutenberg-Universitaet Mainz, Mainz (Germany); Jaworski, Grzegorz; Javier Valiente-Dobon, Jose; De Angelis, Giacomo; Modamio, Victor; Triossi, Andrea [Laboratori Nazionali di Legnaro, Istituto Nazionale di Fisica Nucleare, Legnaro (Italy); Nyberg, Johan [Uppsala University, Department of Physics and Astronomy, Uppsala (Sweden); Palacz, Marcin [University of Warsaw, Heavy Ion Laboratory, Warsaw (Poland); Soederstroem, Paer-Anders [RIKEN Nishina Center, Saitama (Japan); Aliaga-Varea, Ramon Jose [Universidad de Valencia, Instituto de Fisica Corpuscular, CSIC, Paterna (Valencia) (Spain); Universidad Politecnica de Valencia, I3M, Valencia (Spain); Atac, Ayse [Ankara University, Department of Physics, Faculty of Sciences, Ankara (Turkey); The Royal Institute of Technology, Stockholm (Sweden); Collado, Javier; Egea, Francisco Javier; Gonzalez, Vicente; Sanchis, Enrique [University of Valencia, Department of Electronic Engineering, Burjassot (Valencia) (Spain); Erduran, Nizamettin [Istanbul Sabahattin Zaim University, Faculty of Engineering and Natural Sciences, Istanbul (Turkey); Ertuerk, Sefa [University of Nigde, Department of Physics, Faculty of Science and Arts, Nigde (Turkey); France, Gilles de [CNRS/IN2P3, GANIL, CEA/DSAM, Caen (France); Gadea, Rafael; Herrero-Bosch, Vicente [Universidad Politecnica de Valencia, I3M, Valencia (Spain); Kaskas, Ayse [Ankara University, Department of Physics, Faculty of Sciences, Ankara (Turkey); Moszynski, Marek [National Centre for Nuclear Research, Otwock-Swierk (Poland); Wadsworth, Robert [University of York, Department of Physics, York (United Kingdom)

    2016-03-15

    The NEutron Detector Array (NEDA) project aims at the construction of a new high-efficiency compact neutron detector array to be coupled with large γ -ray arrays such as AGATA. The application of NEDA ranges from its use as selective neutron multiplicity filter for fusion-evaporation reaction to a large solid angle neutron tagging device. In the present work, possible configurations for the NEDA coupled with the Neutron Wall for the early implementation with AGATA has been simulated, using Monte Carlo techniques, in order to evaluate their performance figures. The goal of this early NEDA implementation is to improve, with respect to previous instruments, efficiency and capability to select multiplicity for fusion-evaporation reaction channels in which 1, 2 or 3 neutrons are emitted. Each NEDA detector unit has the shape of a regular hexagonal prism with a volume of about 3.23l and it is filled with the EJ301 liquid scintillator, that presents good neutron- γ discrimination properties. The simulations have been performed using a fusion-evaporation event generator that has been validated with a set of experimental data obtained in the {sup 58}Ni + {sup 56}Fe reaction measured with the Neutron Wall detector array. (orig.)

  2. Development of an accelerated test design for predicting the service life of the solar array at Mead, Nebraska. Quarterly report

    Energy Technology Data Exchange (ETDEWEB)

    Gaines, G.B.; Thomas, R.E.; Noel, G.T.; Shilliday, T.S.; Wood, V.E.; Carmichael, D.C.

    1979-02-06

    Economic viability requires that photovoltaic arrays should have a service life of 20 years or longer. Qualification and performance tests indicate that presently available photovoltaic modules provide acceptable performance at the time of installation. This study is being conducted as part of a program to develop and validate an accelerated test plan that can be used to predict the useful service life of present and future solar arrays. Previously a methodology was developed for designing an accelerated test program incorporating trade-offs between the cost of each test and its value in reducing the variance in the life prediction for that array. The objective of the present study is to apply this methodology to develop an accelerated test plan to predict the service life of the 25-kW photovoltaic array installed near Mead, Nebraska. Potential long-term degradation modes for the two types of modules in the Mead array have been determined and judgments have been made as to those environmental stresses and combinations of stresses which accelerate the degradation of the power output. Hierarchical trees representing the severity of effects of stresses (test conditions) on eleven individual degradation modes have been constructed and have been pruned of tests judged to be nonessential. Composites of those trees have been developed so that there is now one pruned tree covering eight degradation modes, another covering two degradation modes, and a third covering one degradation mode. These three composite trees form the basis for selection of test conditions in the final test plan which is now being prepared.

  3. Design and Development of Broadband Inverted E-shaped Patch Microstrip Array Antenna For 3G Wireless Network

    Directory of Open Access Journals (Sweden)

    Norbahiah Misran

    2008-01-01

    Full Text Available Microstrip patch antenna has been received tremendous attention since the last two decades and now it becomes a major component in the development of Smart Antenna System for Third-Generation Wireless Network proposed by the ITU-R under the banner of IMT-2000. Smart antenna consists of an array of antennas associated with it a base-band hardware and control unit (inclusive of the software algorithm that have the capability to change its radiation pattern according to the direction of the user. This paper describes the design and development of broadband Inverted E-shaped patch microstrip array antennas for 3G wireless network. The antenna was designed for the IMT-2000 operating frequency range of 1.885–2.200GHz and was built as an array of 4x4 inverted E-shaped patches. The beamforming feed network comprises of commercial variable attenuators (KAT1D04SA002, variable phase shifters (KPH35OSC000, and the corporate 16-ways Wilkinson power divider which was developed in-house. The antenna successfully achieves the bandwidth of 16.14% (at VSWR: 1.5 with respect to the center frequency of 2.045 GHz. The antenna is capable of scanning with the maximum scanning angle of ±30º and ±25º in azimuth and elevation respectively.

  4. Design and evaluation of a 63 element 1.75-dimensional ultrasound phased array for treating benign prostatic hyperplasia

    Science.gov (United States)

    Saleh, Khaldon Y.; Smith, Nadine B.

    2003-10-01

    Focused ultrasound surgery (FUS) is a clinical method for treating benign prostatic hyperplasia (BPH) in which tissue is noninvasively necrosed by elevating the temperature at the focal point above 60°C using short sonications. With 1.75-dimensional (1.75-D) arrays, the power and phase to the individual elements can be controlled electronically for focusing and steering. This research describes the design, construction and evaluation of a 1.75-D ultrasound phased array to be used in the treatment of benign prostatic hyperplasia. The array was designed with a steering angle of +/-13.5 deg in the transverse direction, and can move the focus in three parallel planes in the longitudinal direction with a relatively large focus size. A piezoelectric ceramic (PZT-8) was used as the material of the transducer and two matching layers were built for maximum acoustic power transmission to tissue. To verify the capability of the transducer for focusing and steering, exposimetry was performed and the results correlated well with the calculated fields. In vivo experiments were performed to verify the capability of the transducer to ablate tissue using short sonications. [Work supported by the Whitaker Foundation and the Department of Defense Congressionally Directed Medical Prostate Cancer Research Program.

  5. Hybrid Differential Evolution with Biogeography-Based Optimization for Design of a Reconfigurable Antenna Array with Discrete Phase Shifters

    Directory of Open Access Journals (Sweden)

    Xiangtao Li

    2011-01-01

    Full Text Available Multibeam antenna arrays have important applications in communications and radar. This paper presents a new method of designing a reconfigurable antenna with quantized phase excitations using a new hybrid algorithm, called DE/BBO. The reconfigurable design problem is to find the element excitation that will result in a sector pattern main beam with low sidelobes with additional requirement that the same excitation amplitudes applied to the array with zero-phase should be in a high directivity, low sidelobe pencil-shaped main beam. In order to reduce the effect of mutual coupling between the antenna-array elements, the dynamic range ratio is minimized. Additionally, compared with the continuous realization and subsequent quantization, experimental results indicate that the performance of the discrete realization of the phase excitation value can be improved. In order to test the performances of hybrid differential evolution with biogeography-based optimization, the results of some state-of-art algorithms are considered, for the purposed of comparison. Experiment results indicate the better performance of the DE/BBO.

  6. Software design for the control system for Small-Size Telescopes with single-mirror of the Cherenkov Telescope Array

    CERN Document Server

    :,; Błocki, J; Bogacz, L; Borkowski, J; Bulik, T; Cadoux, F; Christov, A; Curyło, M; della Volpe, D; Dyrda, M; Favre, Y; Frankowski, A; Grudnik, Ł; Grudzińska, M; Heller, M; Idźkowski, B; Jamrozy, M; Janiak, M; Kasperek, J; Lalik, K; Lyard, E; Mach, E; Mandat, D; Marszałek, A; Michałowski, J; Moderski, R; Rameez, M; Montaruli, T; Neronov, A; Niemiec, J; Ostrowski, M; Paśko, P; Pech, M; Porcelli, A; Prandini, E; Rajda, P; Schioppa, E jr; Schovanek, P; Seweryn, K; Skowron, K; Sliusar, V; Sowiński, M; Stawarz, Ł; Stodulska, M; Stodulski, M; Pujadas, I Troyano; Toscano, S; Walter, R; Wiȩcek, M; Zagdański, A; Ziȩtara, K; Zychowski, P

    2015-01-01

    The Small-Size Telescope with single-mirror (SST-1M) is a 4 m Davies-Cotton telescope and is among the proposed telescope designs for the Cherenkov Telescope Array (CTA). It is conceived to provide the high-energy ($>$ few TeV) coverage. The SST-1M contains proven technology for the telescope structure and innovative electronics and photosensors for the camera. Its design is meant to be simple, low-budget and easy-to-build industrially. Each device subsystem of an SST-1M telescope is made visible to CTA through a dedicated industrial standard server. The software is being developed in collaboration with the CTA Medium-Size Telescopes to ensure compatibility and uniformity of the array control. Early operations of the SST-1M prototype will be performed with a subset of the CTA central array control system based on the Alma Common Software (ACS). The triggered event data are time stamped, formatted and finally transmitted to the CTA data acquisition. The software system developed to control the devices of an SS...

  7. Concept, Design, and Prototyping of XSAS: A High Power Extendable Solar Array for CubeSat Applications

    Science.gov (United States)

    Senatore, Patrick; Klesh, Andrew; Zurbuchen, Thomas H.; McKague, Darren; Cutler, James

    2010-01-01

    CubeSats have proven themselves as a reliable and cost-effective method to perform experiments in space, but they are highly constrained by their specifications and size. One such constraint is the average continuous power, about 5 W, which is available to the typical CubeSat. To improve this constraint, we have developed the eXtendable Solar Array System (XSAS), a deployable solar array prototype in a CubeSat package, which can provide an average 23 W of continuous power. The prototype served as a technology demonstrator for the high risk mechanisms needed to release, deploy, and control the solar array. Aside from this drastic power increase, it is in the integration of each mechanism, their application within the small CubeSat form-factor, and the inherent passive control benefit of the deployed geometry that make XSAS a novel design. In this paper, we discuss the requirements and design process for the XSAS system and mechanical prototype, and provide qualitative and quantitative results from numerical simulations and prototype tests. We also discuss future work, including an upcoming NASA zero-gravity flight campaign, to further improve on XSAS and prepare it for future launch opportunities.

  8. A new approach to design an efficient micropost array for enhanced direct-current insulator-based dielectrophoretic trapping.

    Science.gov (United States)

    Mohammadi, Mahdi; Zare, Mohammad Javad; Madadi, Hojjat; Sellarès, Jordi; Casals-Terré, Jasmina

    2016-07-01

    Direct-current insulator-based dielectrophoresis (DC-iDEP) is a well-known technique that benefits from the electric field gradients generated by an array of insulating posts to separate or trap biological particles. The aim of this study is to provide a first geometrical relationship of the post array that independent of the particles and/or medium, maximizes the trapping. A novel figure of merit is proposed to maximize the particle trapping in the post array while minimizing the required voltage, with a similar footprint and channel thickness. Different post array models with the variation of transversal distance (10 to 60 μm), longitudinal distance (10 to 80 μm), and post radius (10 to 150 μm) were analyzed using COMSOL Multiphysics finite element software. The obtained results indicated that a post radius of 40 μm larger than the transversal distance between posts could enhance the trapping condition between 56 % (for a transversal distance of 10 μm) and 341 % (for a transversal distance of 60 μm). For the validation of the numerical results, several microchannels with embedded post arrays were manufactured in polydimethylsiloxane (PDMS) and the particle trapping patterns of 6-μm-diameter polystyrene particles were measured experimentally. The experiments confirm the same trends as pointed out by the numerical analysis. The results show that this new figure of merit and geometrical relationship can be used to reduce the required electric field to achieve effective particle trapping and, therefore, avoid the negative effects of Joule heating in cells or viable particles. The main advantage of these results is that they depend only on the geometry of the micropost array and are valid for trapping different particles suspended in different media. Graphical abstract Analysis to maximize the particle trapping in the post array while minimizing the required voltage. I. Microfluidic channel design and experimental setup II. Numerical and experimental

  9. 毫米波微带天线阵列设计%Design of a millimeter wave microstrip antenna array

    Institute of Scientific and Technical Information of China (English)

    于慧娟

    2016-01-01

    A circular aperture millimeter wave series and parallel fed microstrip antenna array was proposed. The antenna was designed with rectangular microstrip patch which was fed by slot-coupled to increase the bandwidth. A series-fed microstrip antenna array was selected in order to use the antenna aperture area effectively and decrease the net complexity. At the same time, according to design of the circular aperture millimeter wave micro-strip antenna array, the part array was series and parallel fed to increase the bandwidth of the microstrip antenna. Results show that the bandwidth of the antenna is about 5%. The simulation result of the antenna’s gain is better than 30.6 dBi in working band. The beam width is about 4.0°×3.5° and the side lobe level is lower than –13 dB. Multi beam and phased array functions can be realized with the antenna array and the net.%设计了一个圆口径串并联混合馈电的毫米波微带天线阵列。该天线采用矩形微带工字型缝隙贴片耦合馈电的方法展宽带宽。为了有效利用天线口径面积,减小网络复杂度,选取串联微带天线阵列形式。同时为了展宽带宽,根据设计的圆形口径阵列,将部分子阵采用串并联混合馈电的形式,得到带宽为5%的毫米波微带天线阵列。仿真表明,该天线在工作频带内增益大于30.6 dBi,波束宽度为4.0°×3.5°,副瓣电平低于–13 dB。该天线阵面与网络配合,可以实现多波束或相控阵的功能。

  10. Design, development and evaluation of a resistor-based multiplexing circuit for a 20×20 SiPM array

    Science.gov (United States)

    Wang, Zhonghai; Sun, Xishan; Lou, Kai; Meier, Joseph; Zhou, Rong; Yang, Chaowen; Zhu, Xiaorong; Shao, Yiping

    2016-04-01

    One technical challenge in developing a large-size scintillator detector with multiple Silicon Photomultiplier (SiPM) arrays is to read out a large number of detector output channels. To achieve this, different signal multiplexing circuits have been studied and applied with different performances and cost-effective tradeoffs. Resistor-based multiplexing circuits exhibit simplicity and signal integrity, but also present the disadvantage of timing shift among different channels. In this study, a resistor-based multiplexing circuit for a large-sized SiPM array readout was developed and evaluated by simulation and experimental studies. Similarly to a multiplexing circuit used for multi-anode PMT, grounding and branching resistors were connected to each SiPM output channel. The grounding resistor was used to simultaneously reduce the signal crosstalk among different channels and to improve timing performance. Both grounding and branching resistor values were optimized to maintain a balanced performance of the event energy, timing, and positioning. A multiplexing circuit was implemented on a compact PCB and applied for a flat-panel detector which consisted of a 32×32 LYSO scintillator crystals optically coupled to 5×5 SiPM arrays for a total 20×20 output channels. Test results showed excellent crystal identification for all 1024 LYSO crystals (each with 2×2×30 mm3 size) with 22Na flood-source irradiation. The measured peak-to-valley ratio from typical crystal map profile is around 3:1 to 6.6:1, an average single crystal energy resolution of about 17.3%, and an average single crystal timing resolution of about 2 ns. Timing shift among different crystals, as reported in some other resistor-based multiplexing circuit designs, was not observed. In summary, we have designed and implemented a practical resistor-based multiplexing circuit that can be readily applied for reading out a large SiPM array with good detector performance.

  11. Elimination of mutual inductance in NMR phased arrays: the paddle design revisited.

    Science.gov (United States)

    Constantinides, Christakis; Angeli, Stelios

    2012-09-01

    This study proposes a method to empirically minimize mutual inductance, using passive end-ring circular paddles, with neighboring coil loops placed in a non-overlapped configuration. The proposed concepts are validated through B(1)-field simulations for resonant coils at f(o)=300.5 MHz, having various sizes (3-10 cm), and for paddles with sizes ranging from 16 to 30 mm, and bench tests on constructed 4×4cm(2) two- (1×2) and four-coil loop (2×2) planar arrays. Simulation results yield total mean percentage B(1)-field differences of only 7.03% between the two non-overlapping coil array configurations (paddles vs. no-paddles). Pair-wise comparisons of elicited mean B(1)-field differences from the use of different circular and rectangular paddle sizes, yield values <5.3%. Theoretical calculation of the normalized mutual coupling coefficient in the non-overlapped coil configuration reduces to almost zero with optimally sized-paddles having a radius of approximately 28% the coil's largest dimension. In the absence of paddles, differences in the split of resonance peaks of 9.9 MHz were observed for the two coils in the 1×2 array, which vanished with paddle placement. Single coil responses (unloaded/loaded) without paddles, and responses from array coils with use of optimally-sized paddles yielded quality factor ratios that ranged between 1.1-1.86 and 1.0-1.5, respectively. Phantom and mouse loaded reflection coefficients S(11)/S(22) were -16.7/-16.2dB and -28.2/-16.1 dB, for the two array loops, respectively. Under unloaded conditions and in the absence of paddles, split resonances were observed for the 1×2 array, yielding transmission coefficients of -5.5 to -8.1 dB, reversing to single resonance responses upon paddle placements, with transmission coefficients of -14.4 to -15.6 dB.

  12. Rational design of two-dimensional molecular donor-acceptor nanostructure arrays

    Science.gov (United States)

    Zhang, Jia Lin; Zhong, Shu; Zhong, Jian Qiang; Niu, Tian Chao; Hu, Wen Ping; Wee, Andrew Thye Shen; Chen, Wei

    2015-02-01

    The construction of long-range ordered organic donor-acceptor nanostructure arrays over microscopic areas supported on solid substrates is one of the most challenging tasks towards the realization of molecular nanodevices. They can also be used as ideal model systems to understand light induced charge transfer, charge separation and energy conversion processes and mechanisms at the nanometer scale. The aim of this paper is to highlight recent advances and progress in this topic. Special attention is given to two different strategies for the construction of organic donor-acceptor nanostructure arrays, namely (i) molecular self-assembly on artificially patterned or pre-defined molecular surface nanotemplates and (ii) molecular nanostructure formation steered via directional and selective intermolecular interactions. The interfacial charge transfer and the energy level alignment of these donor-acceptor nanostructures are also discussed.

  13. Design, development, manufacture, testing, and delivery of devices for connection of solar cell panel circuitry to flat conductor cable solar cell array harness

    Science.gov (United States)

    Dillard, P. A.; Waddington, D.

    1971-01-01

    The technology status and problem areas which exist for the application of flat conductor cabling to solar cell arrays are summarized. Details covering the design, connector manufacture, and prototype test results are also summarized.

  14. Comparing temporally-focused GPC and CGH for two-photon excitation and optogenetics in turbid media

    DEFF Research Database (Denmark)

    Villangca, Mark Jayson; Bañas, Andrew Rafael; Aabo, Thomas;

    2013-01-01

    a 4f setup that directly converts phase information to intensity. The GPC method has been used with temporal focusing for excitation in two-photon optogenetics [1-3]. The computer generated hologram (CGH) is also used to generate arbitrary light patterns and has been used for optical manipulation...

  15. Design of crossed-mirror array to form floating 3D LED signs

    Science.gov (United States)

    Yamamoto, Hirotsugu; Bando, Hiroki; Kujime, Ryousuke; Suyama, Shiro

    2012-03-01

    3D representation of digital signage improves its significance and rapid notification of important points. Our goal is to realize floating 3D LED signs. The problem is there is no sufficient device to form floating 3D images from LEDs. LED lamp size is around 1 cm including wiring and substrates. Such large pitch increases display size and sometimes spoils image quality. The purpose of this paper is to develop optical device to meet the three requirements and to demonstrate floating 3D arrays of LEDs. We analytically investigate image formation by a crossed mirror structure with aerial aperture, called CMA (crossed-mirror array). CMA contains dihedral corner reflectors at each aperture. After double reflection, light rays emitted from an LED will converge into the corresponding image point. We have fabricated CMA for 3D array of LEDs. One CMA unit contains 20 x 20 apertures that are located diagonally. Floating image of LEDs was formed in wide range of incident angle. The image size of focused beam agreed to the apparent aperture size. When LEDs were located three-dimensionally (LEDs in three depths), the focused distances were the same as the distance between the real LED and the CMA.

  16. Design of a stacked array antenna system integrated with low temperature co-fired ceramics (LTCC)

    Science.gov (United States)

    Ji, Taeksoo; Yoon, Hargsoon; Jose, K. A.; Varadan, Vijay K.

    2005-05-01

    In this paper, we presents a 4×4 stacked phased array antenna system operating at 15GHz, which can be used for commercial as well as military applications including low earth orbiting (LEO) satellites communications and airborne defense system. The phased array antenna consists of 4 subarrays having 4 tapered slot antennas, phase shifters, power dividers, and high voltage controllers. Each component is constructed on low temperature co-fired ceramics (LTCC) that is a multilayer electronic packaging technology and has a unique ability to integrate passive components such as resistors, capacitors and inductors in to a monolithic package. The phase shifter we have developed herein using barium strontium titanate (BST) thin films shows continuous phase shifts of 0°~90° at 15GHz when DC bias voltages are applied up to 300 V between the ground and signal line. By controlling the voltages independently applied to each phase shifters, the beam shape and direction radiated from the array antenna can be changed and steered.

  17. 1-Mb resolution array-based comparative genomic hybridization using a BAC clone set optimized for cancer gene analysis

    NARCIS (Netherlands)

    Greshock, J; Naylor, TL; Margolin, A; Diskin, S; Cleaver, SH; Futreal, PA; deJong, PJ; Zhao, SY; Liebman, M; Weber, BL

    2004-01-01

    Array-based comparative genomic hybridization (aCGH) is a recently developed tool for genome-wide determination of DNA copy number alterations. This technology has tremendous potential for disease-gene discovery in cancer and developmental disorders as well as numerous other applications. However, w

  18. High-contrast pattern reconstructions using a phase-seeded point CGH method.

    Science.gov (United States)

    McWilliam, Richard; Williams, Gavin L; Cowling, Joshua J; Seed, Nicholas L; Purvis, Alan

    2016-03-01

    A major challenge encountered in digital holography applications is the need to synthesize computer-generated holograms (CGHs) that are realizable as phase-only elements while also delivering high quality reconstruction. This trade-off is particularly acute in high-precision applications such as photolithography where contrast typically must exceed 0.6. A seeded-phase point method is proposed to address this challenge, whereby patterns composed of fine lines that intersect and form closed shapes are reconstructed with high contrast while maintaining a phase-only CGH. The method achieves superior contrast to that obtained by uniform or random seeded-phase methods while maintaining computational efficiency for large area exposures. It is also shown that binary phase modulation achieves similar contrast performance with benefits for the fabrication of simpler diffractive optical elements. PMID:26974633

  19. Design of a Weighted-Rotor Energy Harvester Based on Dynamic Analysis and Optimization of Circular Halbach Array Magnetic Disk

    Directory of Open Access Journals (Sweden)

    Yu-Jen Wang

    2015-03-01

    Full Text Available This paper proposes the design of a weighted-rotor energy harvester (WREH in which the oscillation is caused by the periodic change of the tangential component of gravity, to harvest kinetic energy from a rotating wheel. When a WREH is designed with a suitable characteristic length, the rotor’s natural frequency changes according to the wheel rotation speed and the rotor oscillates at a wide angle and high angular velocity to generate a large amount of power. The magnetic disk is designed according to an optimized circular Halbach array. The optimized circular Halbach array magnetic disk provides the largest induced EMF for different sector-angle ratios for the same magnetic disk volume. This study examined the output voltage and power by considering the constant and accelerating plate-rotation speeds, respectively. This paper discusses the effects of the angular acceleration speed of a rotating wheel corresponding to the dynamic behaviors of a weighted rotor. The average output power is 399 to 535 microwatts at plate-rotation speeds from 300 to 500 rpm, enabling the WREH to be a suitable power source for a tire-pressure monitoring system.

  20. Optimizing and Improving the Growth Quality of ZnO Nanowire Arrays Guided by Statistical Design of Experiments.

    Science.gov (United States)

    Xu, Sheng; Adiga, Nagesh; Ba, Shan; Dasgupta, Tirthankar; Wu, C F Jeff; Wang, Zhong Lin

    2009-07-28

    Controlling the morphology of the as-synthesized nanostructures is usually challenging, and there lacks of a general theoretical guidance in experimental approach. In this study, a novel way of optimizing the aspect ratio of hydrothermally grown ZnO nanowire (NW) arrays is presented by utilizing a systematic statistical design and analysis method. In this work, we use pick-the-winner rule and one-pair-at-a-time main effect analysis to sequentially design the experiments and identify optimal reaction settings. By controlling the hydrothermal reaction parameters (reaction temperature, time, precursor concentration, and capping agent), we improved the aspect ratio of ZnO NWs from around 10 to nearly 23. The effect of noise on the experimental results was identified and successfully reduced, and the statistical design and analysis methods were very effective in reducing the number of experiments performed and in identifying the optimal experimental settings. In addition, the antireflection spectrum of the as-synthesized ZnO NWs clearly shows that higher aspect ratio of the ZnO NW arrays leads to about 30% stronger suppression in the UV-vis range emission. This shows great potential applications as antireflective coating layers in photovoltaic devices.

  1. Accurate two-dimensional model of an arrayed-waveguide grating demultiplexer and optimal design based on the reciprocity theory.

    Science.gov (United States)

    Dai, Daoxin; He, Sailing

    2004-12-01

    An accurate two-dimensional (2D) model is introduced for the simulation of an arrayed-waveguide grating (AWG) demultiplexer by integrating the field distribution along the vertical direction. The equivalent 2D model has almost the same accuracy as the original three-dimensional model and is more accurate for the AWG considered here than the conventional 2D model based on the effective-index method. To further improve the computational efficiency, the reciprocity theory is applied to the optimal design of a flat-top AWG demultiplexer with a special input structure.

  2. Design and fabrication of a multi-purpose soft x-ray array diagnostic system for KSTAR.

    Science.gov (United States)

    Lee, Seung Hun; Chai, Kil Byoung; Jang, Siwon; Ko, Won-Ha; Kim, Junghee; Seo, Dongcheol; Lee, Jongha; Bogatu, I N; Kim, Jin-Soo; Choe, Wonho

    2012-10-01

    A multi-purpose soft x-ray array diagnostic system was developed for measuring two-dimensional x-ray emissivity profile, electron temperature, Ar impurity transport, and total radiation power. A remotely controlled filter wheel was designed with three different choices of filters. The electron temperature profile can be determined from the ratio of two channels having different thickness of Be layer, and the Ar impurity concentration transport can be determined from a pair of Ar Ross filters (CaF(2) and NaCl thin films). Without any filters, this diagnostic system can also be used as a bolometer.

  3. Design and fabrication of the thin-film micromirror array-actuated for large projection displays

    CERN Document Server

    Hwang, K H; Kim, S G

    1998-01-01

    The Thin-film Micromirror Array-actuated (TMA) is a new reflective type light modulator that uses micromachined thin film piezoelectric actuators in conjunction with over 300,000 micromirrors. A working prototype of TMA display system with three TMA modules shows the world best light efficiency at the present time, which is more than 7 lumen per watt light efficiency. Projectors and projection TVs with TMAs will be the most promising device which will provide clear display of electronic image at normal room light condition.

  4. Design of a Dielectric Rod Waveguide Antenna Array for Millimeter Waves

    Science.gov (United States)

    Rivera-Lavado, Alejandro; García-Muñoz, Luis-Enrique; Generalov, Andrey; Lioubtchenko, Dmitri; Abdalmalak, Kerlos-Atia; Llorente-Romano, Sergio; García-Lampérez, Alejandro; Segovia-Vargas, Daniel; Räisänen, Antti V.

    2016-09-01

    In this manuscript, the use of dielectric rod waveguide (DRW) antennas in the millimeter and sub-millimeter wave range is presented as a solution for covering two issues: getting more radiated power and filling a technological gap problem in the terahertz band, namely a fully electronic beam steering. A 4x4 element array working at 100 GHz fed by a rectangular waveguide is manufactured and measured for showing its capabilities. This topology can be used as a cost-affordable alternative to dielectric lenses in photomixer-based terahertz sources.

  5. Design and optimization of micro-semiconductor bridge used for solid propellant microthrusters array

    Science.gov (United States)

    Ru, Chengbo; Dai, Ji; Xu, Jianbing; Ye, Yinghua; Zhu, Peng; Shen, Ruiqi

    2016-06-01

    Igniter is the basic component of MEMS-based solid propellant microthrusters (SPM) array, which should response fast with low dissipated energy. To satisfy the requirements, micro-semiconductor bridge (MSCB) with dual V-angles contributing to the reduction of dissipated energy is introduced into the array. The electrical explosion characteristics of MSCB investigated under constant voltage, in the considerations of the limit of power supply in the micro/nanosatellite, are similar to those under capacitive discharge. The bridge was completely vaporized and burst into the hot plasma under high-level voltage. While under low-level voltage, the bridge was partly vaporized without detected plasma. The 3#SCB (90° V-angle) with the smallest power density resulting in the lowest applied voltage (12.0 V) and the 2#SCB with the smallest narrow width (Wmin) leading to the lowest average power (11.3 W) were chosen to the further optimization. The ignition tests were implemented to examine the ignition reliability of the contractible igniters, which were optimized to satisfy the voltage limitation with sufficient ignition capacity. The results show that the effective SCB with lowest dissipated power is the 3#-3SCB, which function within 100 μ under 7.0 V, and the average power below 5.0 W.

  6. Design and operation of a portable scanner for high performance microchip capillary array electrophoresis

    Science.gov (United States)

    Scherer, James R.; Liu, Peng; Mathies, Richard A.

    2010-11-01

    We have developed a compact, laser-induced fluorescence detection scanner, the multichannel capillary array electrophoresis portable scanner (McCAEPs) as a platform for electrophoretic detection and control of high-throughput, integrated microfluidic devices for genetic and other analyses. The instrument contains a confocal optical system with a rotary objective for detecting four different fluorescence signals, a pneumatic system consisting of two pressure/vacuum pumps and 28 individual addressable solenoid valves for control of on-chip microvalves and micropumps, four Polymerase Chain Reaction (PCR) temperature control systems, and four high voltage power supplies for electrophoresis. The detection limit of the instrument is ˜20 pM for on-chip capillary electrophoresis of fluorescein dyes. To demonstrate the system performance for forensic short tandem repeat (STR) analysis, two experiments were conducted: (i) electrophoretic separation and detection of STR samples on a 96-lane microfabricated capillary array electrophoresis microchip. Fully resolved PowerPlex® 16 STR profiles amplified from 1 ng of 9947A female standard DNA were successfully obtained; (ii) nine-plex STR amplification, sample injection, separation, and fluorescence detection of 100-copy 9948 male standard DNA in a single integrated PCR- capillary electrophoresis microchip. These results demonstrate that the McCAEPs can be used as a versatile control and detection instrument that operates integrated microfluidic devices for high-performance forensic human identification.

  7. LED array designing and its bactericidal effect researching on Pseudomonas aeruginosa in vitro

    Science.gov (United States)

    Fang, Jing; Xing, Jin; Gao, Liucun; Shen, Benjian; Kang, Hongxiang; Jie, Liang; Peng, Chen

    2015-10-01

    Lights with some special waveband and output power density have a bactericidal effect to some special bacteria. In this paper, the bactericidal effect of light at wavelength of 470 nm on P. aeruginosa (ATCC 27853) is researched with different irradiation dose. The light source is a LED array which is obtained by incoherent combine of 36 LEDs with emitting wavelength of 470 nm. The P. aeruginosa suspension is exposed with the LED array at the light power density of 100 mW/cm2 with exposures time of 0, 5, 10, 20, 40, and 80 min, respectively. The numbers of CFU are then determined by serial dilutions on LB agar plates. The bactericidal effect research results of 470 nm LED on P. aeruginosa show that the killing ratio increases with increasing of the exposure time. For the 80 min irradiation, as much as 92.4% reduction of P. aeruginosa is achieved. The results indicate that, in vitro, 470-nm lights produce dose dependent bactericidal effects on P. aeruginosa.

  8. Design and Performance of a TES X-ray Microcalorimeter Array for Energy Dispersive Spectroscopy on Scanning Transmission Electron Microscope

    Science.gov (United States)

    Muramatsu, Haruka; Nagayoshi, K.; Hayashi, T.; Sakai, K.; Yamamoto, R.; Mitsuda, K.; Yamasaki, N. Y.; Maehata, K.; Hara, T.

    2016-07-01

    We discuss the design and performance of a transition edge sensor (TES) X-ray microcalorimeter array for scanning transmission electron microscope (STEM)-energy dispersive X-ray spectroscopy (EDS). The TES X-ray microcalorimeter has better energy resolution compared to conventional silicon drift detector and STEM-EDS utilizing a TES detector makes it possible to map the distribution of elements on a specimen in addition to analyze the composition. The requirement for a TES detector is a high counting rate (>20 kcps), wide energy band (0.5-15 keV) and good energy resolution (<10 eV) full width at half maximum. The major improvement of this development is to increase the maximum counting rate. In order to accommodate the high counting rate, we adopted an 8 × 8 format, 64-pixel array and common biasing scheme for the readout method. We did all design and fabrication of the device in house. With the device we have fabricated most recently, the pulse decay time is 40 \\upmu s which is expected to achieve 50 kcps. For a single pixel, the measured energy resolution was 7.8 eV at 5.9 keV. This device satisfies the requirements of counting rate and energy resolution, although several issues remain where the performance must be confirmed.

  9. Analysis of Chinese women with primary ovarian insufficiency by high resolution array-comparative genomic hybridization

    Institute of Scientific and Technical Information of China (English)

    LIAO Can; FU Fang; YANG Xin; SUN Yi-min; LI Dong-zhi

    2011-01-01

    Background Primary ovarian insufficiency (POI) is defined as a primary ovarian defect characterized by absent menarche (primary amenorrhea) or premature depletion of ovarian follicles before the age of 40 years. The etiology of primary ovarian insufficiency in human female patients is still unclear. The purpose of this study is to investigate the potential genetic causes in primary amenorrhea patients by high resolution array based comparative genomic hybridization (array-CGH) analysis.Methods Following the standard karyotyping analysis, genomic DNA from whole blood of 15 primary amenorrhea patients and 15 normal control women was hybridized with Affymetrix cytogenetic 2.7M arrays following the standard protocol. Copy number variations identified by array-CGH were confirmed by real time polymerase chain reaction.Results All the 30 samples were negative by conventional karyotyping analysis. Microdeletions on chromosome 17q21.31-q21.32 with approximately 1.3 Mb were identified in four patients by high resolution array-CGH analysis. This included the female reproductive secretory pathway related factor N-ethylmaleimide-sensitive factor (NSF) gene.Conclusions The results of the present study suggest that there may be critical regions regulating primary ovarian insufficiency in women with a 17q21.31-q21.32 microdeletion. This effect might be due to the loss of function of the NSF gene/genes within the deleted region or to effects on contiguous genes.

  10. Gimbals Drive and Control Electronics Design, Development and Testing of the LRO High Gain Antenna and Solar Array Systems

    Science.gov (United States)

    Chernyakov, Boris; Thakore, Kamal

    2010-01-01

    Launched June 18, 2009 on an Atlas V rocket, NASA's Lunar Reconnaissance Orbiter (LRO) is the first step in NASA's Vision for Space Exploration program and for a human return to the Moon. The spacecraft (SC) carries a wide variety of scientific instruments and provides an extraordinary opportunity to study the lunar landscape at resolutions and over time scales never achieved before. The spacecraft systems are designed to enable achievement of LRO's mission requirements. To that end, LRO's mechanical system employed two two-axis gimbal assemblies used to drive the deployment and articulation of the Solar Array System (SAS) and the High Gain Antenna System (HGAS). This paper describes the design, development, integration, and testing of Gimbal Control Electronics (GCE) and Actuators for both the HGAS and SAS systems, as well as flight testing during the on-orbit commissioning phase and lessons learned.

  11. Design of Logic Module Based on Magnetic-Tunnel-Junction Elements for Nonvolatile Field-Programmable Gate Array

    Science.gov (United States)

    Lee, Hyunjoo; Kim, Sojeong; Lee, Seungyeon; Lee, Seungjun; Shin, Hyungsoon

    2009-04-01

    Magnetologic using a magnetic-tunnel-junction (MTJ) element is a very hopeful candidate for universal logic technology because it can be used to build both logic circuits and nonvolatile memories. A structure of single-layer (SL) MTJ with a novel current driver previously presented by the authors improved both functional flexibility and uniformity of magnetologic. In this paper, the design of a nonvolatile logic module using SL MTJ is presented, which can be used as a basic logic cell for nonvolatile field-programmable gate arrays (FPGAs). The S-module is a basic logic cell for Act3 family of FPGAs by Actel, which can implement arbitrary five-input logic functions. We designed an S-module using SL MTJ elements such that it can work as a programmable logic module with nonvolatility. The functional verification has been carried out by HSPICE simulator on the basis of a macro-model of SL MTJ.

  12. Analysis and design of low noise column stage in CMOS ROIC for UV GaN focal plane array

    Science.gov (United States)

    Li, Xiaojuan; Yuan, Yonggang; Xie, Jing; Wang, Jiqiang; Ma, Ding; Wang, Ling; Li, Xiangyang

    2015-03-01

    A novel column-stage structure of readout integrated circuit (ROIC) for GaN ultraviolet (UV) focal plane array (FPA) working in "solar-blind" band is proposed. The column stage has better drive capability, higher dynamic range, stable bias current and low impedance. The noise voltage of the column readout stage is discussed, which has been reduced by small-current driving, column-stage sample and hold and the technology of divided-output-bus. This research on low-noise ROIC is designed for weak-current UV FPA. It is designed, simulated and laid out using the 0.35um 2P4M CMOS 5V process. The clock rate operates at 8MHz. The simulation input current sets 0.01nA. The output swing is 2.6V and power consumption is 40 mW according to the measurement results.

  13. Cross-species comparison of aCGH data from mouse and human BRCA1- and BRCA2-mutated breast cancers

    International Nuclear Information System (INIS)

    Genomic gains and losses are a result of genomic instability in many types of cancers. BRCA1- and BRCA2-mutated breast cancers are associated with increased amounts of chromosomal aberrations, presumably due their functions in genome repair. Some of these genomic aberrations may harbor genes whose absence or overexpression may give rise to cellular growth advantage. So far, it has not been easy to identify the driver genes underlying gains and losses. A powerful approach to identify these driver genes could be a cross-species comparison of array comparative genomic hybridization (aCGH) data from cognate mouse and human tumors. Orthologous regions of mouse and human tumors that are commonly gained or lost might represent essential genomic regions selected for gain or loss during tumor development. To identify genomic regions that are associated with BRCA1- and BRCA2-mutated breast cancers we compared aCGH data from 130 mouse Brca1Δ/Δ;p53Δ/Δ, Brca2Δ/Δ;p53Δ/Δ and p53Δ/Δ mammary tumor groups with 103 human BRCA1-mutated, BRCA2-mutated and non-hereditary breast cancers. Our genome-wide cross-species analysis yielded a complete collection of loci and genes that are commonly gained or lost in mouse and human breast cancer. Principal common CNAs were the well known MYC-associated gain and RB1/INTS6-associated loss that occurred in all mouse and human tumor groups, and the AURKA-associated gain occurred in BRCA2-related tumors from both species. However, there were also important differences between tumor profiles of both species, such as the prominent gain on chromosome 10 in mouse Brca2Δ/Δ;p53Δ/Δ tumors and the PIK3CA associated 3q gain in human BRCA1-mutated tumors, which occurred in tumors from one species but not in tumors from the other species. This disparity in recurrent aberrations in mouse and human tumors might be due to differences in tumor cell type or genomic organization between both species. The selection of the oncogenome during mouse and

  14. Design and Implementation of Video Shot Detection on Field Programmable Gate Arrays

    Directory of Open Access Journals (Sweden)

    Jharna Majumdar

    2012-09-01

    Full Text Available Video has become an interactive medium of communication in everyday life. The sheer volume of video makes it extremely difficult to browse through and find the required data. Hence extraction of key frames from the video which represents the abstract of the entire video becomes necessary. The aim of the video shot detection is to find the position of the shot boundaries, so that key frames can be selected from each shot for subsequent processing such as video summarization, indexing etc. For most of the surveillance applications like video summery, face recognition etc., the hardware (real time implementation of these algorithms becomes necessary. Here in this paper we present the architecture for simultaneous accessing of consecutive frames, which are then used for the implementation of various Video Shot Detection algorithms. We also present the real time implementation of three video shot detection algorithms using the above mentioned architecture on FPGA (Field Programmable Gate Arrays.

  15. The Murchison Widefield Array 21cm Epoch of Reionization Experiment: Design, Construction, and First Season Results

    Science.gov (United States)

    Beardsley, Adam

    The Cosmic Dark Ages and the Epoch of Reionization (EoR) remain largely unexplored chapters in the history and evolution of the Universe. These periods hold the potential to inform our picture of the cosmos similar to what the Cosmic Microwave Background has done over the past several decades. A promising method to probe the neutral hydrogen gas between early galaxies is known as 21cm tomography, which utilizes the ubiquitous hyper-fine transition of HI to create 3D maps of the intergalactic medium. The Murchison Widefield Array (MWA) is an instrument built with a primary science driver to detect and characterize the EoR through 21cm tomography. In this thesis we explore the challenges faced by the MWA from the layout of antennas, to a custom analysis pipeline, to bridging the gap with probes at other wavelengths. We discuss many lessons learned in the course of reducing MWA data with an extremely precise measurement in mind, and conclude with the first deep integration from array. We present a 2-σ upper limit on the EoR power spectrum of Δ^2(k)<1.25×10^4 mK^2 at cosmic scale k=0.236 h Mpc^{-1} and redshift z=6.8. Our result is a marginal improvement over previous MWA results and consistent with the best published limits from other instruments. This result is the deepest imaging power spectrum to date, and is a major step forward for this type of analysis. While our limit is dominated by systematics, we offer strategies for improvement for future analysis.

  16. Problems on design of computer-generated holograms for testing aspheric surfaces: principle and calculation

    Institute of Scientific and Technical Information of China (English)

    Zhishan Gao; Meimei Kong; Rihong Zhu; Lei Chen

    2007-01-01

    Interferometric optical testing using computer-generated hologram (CGH) has provided an approach to highly accurate measurement of aspheric surfaces. While designing the CGH null correctors, we should make them with as small aperture and low spatial frequency as possible, and with no zero slope of phase except at center, for the sake of insuring lowisk of substrate figure error and feasibility of fabrication. On the basis of classic optics, a set of equations for calculating the phase function of CGH are obtained. These equations lead us to find the dependence of the aperture and spatial frequency on the axial diszance from the tested aspheric surface for the CGH. We also simulatethe ptical path difference error of the CGH relative to the accuracy of controlling laser spot during fabrication. Meanwhile, we discuss the constraints used to avoid zero slope of phase except at center and give a design result of the CGH for the tested aspheric surface. The results ensure the feasibility of designing a useful CGH to test aspheric urface fundamentally.

  17. Targeted Resequencing of 9p in Acute Lymphoblastic Leukemia Yields Concordant Results with Array CGH and Reveals Novel Genomic Alterations

    NARCIS (Netherlands)

    Sarhadi, V.K.; Lahti, L.M.; Scheinin, I.; Tyybäkinoja, A.; Savola, S.; Usvasalo, A.; Räty, R.; Elonen, E.; Saarinen-Pihkala, U.M.; Knuutila, S.

    2013-01-01

    Genetic alterations of the short arm of chromosome 9 are frequent in acute lymphoblastic leukemia. We performed targeted sequencing of 9p region in 35 adolescent and adult acute lymphoblastic leukemia patients and sought to investigate the sensitivity of detecting copy number alterations in comparis

  18. Genotype-phenotype correlation in 21 patients with Wolf-Hirschhorn syndrome using high resolution array comparative genome hybridisation (CGH)

    NARCIS (Netherlands)

    Maas, N. M. C.; Van Buggenhout, G.; Hannes, F.; Thienpont, B.; Sanlaville, D.; Kok, K.; Midro, A.; Andrieux, J.; Anderlid, B-M; Schoumans, J.; Hordijk, R.; Devriendt, K.; Fryns, J-P; Vermeesch, J. R.

    2008-01-01

    Background: The Wolf-Hirschhorn syndrome (WHS) is usually caused by terminal deletions of the short arm of chromosome 4 and is phenotypically defined by growth and mental retardation, seizures, and specific craniofacial manifestations. Large variation is observed in phenotypic expression of these fe

  19. Methodology for designing accelerated aging tests for predicting life of photovoltaic arrays

    Science.gov (United States)

    Gaines, G. B.; Thomas, R. E.; Derringer, G. C.; Kistler, C. W.; Bigg, D. M.; Carmichael, D. C.

    1977-01-01

    A methodology for designing aging tests in which life prediction was paramount was developed. The methodology builds upon experience with regard to aging behavior in those material classes which are expected to be utilized as encapsulant elements, viz., glasses and polymers, and upon experience with the design of aging tests. The experiences were reviewed, and results are discussed in detail.

  20. Development of novel on-chip, customer-design spiral biasing adaptor on for Si drift detectors and detector arrays for X-ray and nuclear physics experiments

    Energy Technology Data Exchange (ETDEWEB)

    Li, Zheng, E-mail: lizheng@xtu.edu.cn [School of Materials, Optoelectronics and Physics, Xiangtan University, Xiangtan, Hunan 411105 (China); Chen, Wei [Brookhaven National Laboratory, Upton, NY 11973 (United States)

    2014-11-21

    A novel on-chip, customer-design spiral biasing adaptor (SBA) has been developed. A single SBA is used for biasing a Si drift detector (SDD) and SDD array. The use of an SBA reduces the biasing current. This paper shows the calculation of the geometry of an SBA and an SDD to get the best drift field in the SDD and SDD array. Prototype SBAs have been fabricated to verify the concept. Electrical measurements on these SBAs are in agreement with the expectations. The new SDD array with an SBA can be used for X-ray detection and in nuclear physics experiments.

  1. Increasing Live Birth Rate by Preimplantation Genetic Screening of Pooled Polar Bodies Using Array Comparative Genomic Hybridization

    OpenAIRE

    Michael Feichtinger; Tina Stopp; Christian Göbl; Elisabeth Feichtinger; Enrico Vaccari; Ulrike Mädel; Franco Laccone; Monika Stroh-Weigert; Markus Hengstschläger; Wilfried Feichtinger; Jürgen Neesen

    2015-01-01

    Meiotic errors during oocyte maturation are considered the major contributors to embryonic aneuploidy and failures in human IVF treatment. Various technologies have been developed to screen polar bodies, blastomeres and trophectoderm cells for chromosomal aberrations. Array-CGH analysis using bacterial artificial chromosome (BAC) arrays is widely applied for preimplantation genetic diagnosis (PGD) using single cells. Recently, an increase in the pregnancy rate has been demonstrated using arra...

  2. Design and simulation of a micromirror array for a projection TV

    Science.gov (United States)

    Choi, Bumkyoo; Lee, Junghoon; Jung, Kyuwon; Shin, Hyungjae

    1999-10-01

    The design of a micromirror for a projection TV is investigated. A static structural analysis is performed to give an optimal shape of the micromirror using the FEM commercial package, ANSYS. A solid modeling is created, and mapped meshes are applied to it in order to satisfy a symmetric condition. A stress analysis shows that maximum stress does not exceed an allowable stress, which is the yield strength. A modal analysis is also executed to find the approximate natural frequencies with different design parameters. The result can be utilized to see which design parameter is strongly dominant. The micromirror was fabricated by Samsung Electronics. Dynamic deflection experiments confirm the results of the simulation.

  3. Design, Construction, and Initial Test of High Spatial Resolution Thermometry Arrays for Detection of Surface Temperature Profiles on SRF Cavities in Super Fluid Helium

    Energy Technology Data Exchange (ETDEWEB)

    Ari Palczewski, Rongli Geng, Grigory Eremeev

    2011-07-01

    We designed and built two high resolution (0.6-0.55mm special resolution [1.1-1.2mm separation]) thermometry arrays prototypes out of the Allen Bradley 90-120 ohm 1/8 watt resistor to measure surface temperature profiles on SRF cavities. One array was designed to be physically flexible and conform to any location on a SRF cavity; the other was modeled after the common G-10/stycast 2850 thermometer and designed to fit on the equator of an ILC (Tesla 1.3GHz) SRF cavity. We will discuss the advantages and disadvantages of each array and their construction. In addition we will present a case study of the arrays performance on a real SRF cavity TB9NR001. TB9NR001 presented a unique opportunity to test the performance of each array as it contained a dual (4mm separation) cat eye defect which conventional methods such as OST (Oscillating Superleak second-sound Transducers) and full coverage thermometry mapping were unable to distinguish between. We will discuss the new arrays ability to distinguish between the two defects and their preheating performance.

  4. Study on solder joint reliability of ceramic ball grid array component based on design of experiment method

    Institute of Scientific and Technical Information of China (English)

    Wu Zhaohua; Huang Chunyue; Zhou Dejian

    2006-01-01

    Four process parameters, pad diameter, stencil thickness, ball diameter and stand-off were chosen as four control factors.By using an L25 ( 56 ) orthogonal array the ceramic ball grid array ( CBGA ) solder joints which have 25 different combinations of process parameters were designed.The numerical models of all the 25 CBGA solder joints were developed using the Surface Evolver.Utilizing the surface coordinate exported from the 25 CBGA solder joints numerical models, the finite element analysis models were set up and the nonlinear finite element analysis of the CBGA solder joints under thermal cycles were performed by ANSYS.The thermal fatigne life of CBGA solder joint was calculated using Coffin-Manson equation.Based on the calculated thermal fatigue life results, the range analysis and the variance analysis were performed.The results show that the fatigue life of CBGA solder joint is affected by the pad diameter, the stencil thickness, the ball diameter and the standoff in a descending order, the best combination of process parameters results in the longest fatigue life is 0.07 mm stand-off,0.125 mm stencil thickness of, 0.85 mm ball diameter and 0.89 mm pad diameter.With 95% confidence the pad diameter has a significant effect on the reliability of CBGA solder joints whereas the stand-off, the stencil thickness and the ball diameter have little effect on the reliability of CBGA solder joints.

  5. Method to detect differentially methylated loci with case-control designs using Illumina arrays.

    Science.gov (United States)

    Wang, Shuang

    2011-11-01

    It is now understood that many human cancer types are the result of the accumulation of both genetic and epigenetic changes. DNA methylation is a molecular modification of DNA that is crucial for normal development. Genes that are rich in CpG dinucleotides are usually not methylated in normal tissues, but are frequently hypermethylated in cancer. With the advent of high-throughput platforms, large-scale structure of genomic methylation patterns is available through genome-wide scans and tremendous amount of DNA methylation data have been recently generated. However, sophisticated statistical methods to handle complex DNA methylation data are very limited. Here, we developed a likelihood based Uniform-Normal-mixture model to select differentially methylated loci between case and control groups using Illumina arrays. The idea is to model the data as three types of methylation loci, one unmethylated, one completely methylated, and one partially methylated. A three-component mixture model with two Uniform distributions and one truncated normal distribution was used to model the three types. The mixture probabilities and the mean of the normal distribution were used to make inference about differentially methylated loci. Through extensive simulation studies, we demonstrated the feasibility and power of the proposed method. An application to a recently published study on ovarian cancer identified several methylation loci that are missed by the existing method. PMID:21818777

  6. Analysis of Reverse Phase Protein Array Data: From Experimental Design towards Targeted Biomarker Discovery

    Directory of Open Access Journals (Sweden)

    Astrid Wachter

    2015-11-01

    Full Text Available Mastering the systematic analysis of tumor tissues on a large scale has long been a technical challenge for proteomics. In 2001, reverse phase protein arrays (RPPA were added to the repertoire of existing immunoassays, which, for the first time, allowed a profiling of minute amounts of tumor lysates even after microdissection. A characteristic feature of RPPA is its outstanding sample capacity permitting the analysis of thousands of samples in parallel as a routine task. Until today, the RPPA approach has matured to a robust and highly sensitive high-throughput platform, which is ideally suited for biomarker discovery. Concomitant with technical advancements, new bioinformatic tools were developed for data normalization and data analysis as outlined in detail in this review. Furthermore, biomarker signatures obtained by different RPPA screens were compared with another or with that obtained by other proteomic formats, if possible. Options for overcoming the downside of RPPA, which is the need to steadily validate new antibody batches, will be discussed. Finally, a debate on using RPPA to advance personalized medicine will conclude this article.

  7. The Baryon Acoustic Oscillation Broadband and Broad-beam Array: Design Overview and Sensitivity Forecasts

    CERN Document Server

    Pober, Jonathan C; DeBoer, David R; McDonald, Patrick; McQuinn, Matthew; Aguirre, James E; Ali, Zaki; Bradley, Richard F; Chang, Tzu-Ching; Morales, Miguel F

    2012-01-01

    This work describes a new instrument optimized for a detection of the neutral hydrogen 21cm power spectrum between redshifts of 0.5-1.5: the Baryon Acoustic Oscillation Broadband and Broad-beam (BAOBAB) Array. BAOBAB will build on the efforts of a first generation of 21cm experiments which are targeting a detection of the signal from the Epoch of Reionization at z ~ 10. At z ~ 1, the emission from neutral hydrogen in self-shielded overdense halos also presents an accessible signal, since the dominant, synchrotron foreground emission is considerably fainter than at redshift 10. The principle science driver for these observations are Baryon Acoustic Oscillations in the matter power spectrum which have the potential to act as a standard ruler and constrain the nature of dark energy. BAOBAB will fully correlate dual-polarization antenna tiles over the 600-900MHz band with a frequency resolution of 300 kHz and a system temperature of 50K. The number of antennas will grow in staged deployments, and reconfigurations...

  8. Objective assessment and design improvement of a staring, sparse transducer array by the spatial crosstalk matrix for 3D photoacoustic tomography.

    Directory of Open Access Journals (Sweden)

    Philip Wong

    Full Text Available Accurate reconstruction of 3D photoacoustic (PA images requires detection of photoacoustic signals from many angles. Several groups have adopted staring ultrasound arrays, but assessment of array performance has been limited. We previously reported on a method to calibrate a 3D PA tomography (PAT staring array system and analyze system performance using singular value decomposition (SVD. The developed SVD metric, however, was impractical for large system matrices, which are typical of 3D PAT problems. The present study consisted of two main objectives. The first objective aimed to introduce the crosstalk matrix concept to the field of PAT for system design. Figures-of-merit utilized in this study were root mean square error, peak signal-to-noise ratio, mean absolute error, and a three dimensional structural similarity index, which were derived between the normalized spatial crosstalk matrix and the identity matrix. The applicability of this approach for 3D PAT was validated by observing the response of the figures-of-merit in relation to well-understood PAT sampling characteristics (i.e. spatial and temporal sampling rate. The second objective aimed to utilize the figures-of-merit to characterize and improve the performance of a near-spherical staring array design. Transducer arrangement, array radius, and array angular coverage were the design parameters examined. We observed that the performance of a 129-element staring transducer array for 3D PAT could be improved by selection of optimal values of the design parameters. The results suggested that this formulation could be used to objectively characterize 3D PAT system performance and would enable the development of efficient strategies for system design optimization.

  9. Explorative data analysis of MCL reveals gene expression networks implicated in survival and prognosis supported by explorative CGH analysis

    Directory of Open Access Journals (Sweden)

    Müller-Hermelink Hans K

    2008-04-01

    Full Text Available Abstract Background Mantle cell lymphoma (MCL is an incurable B cell lymphoma and accounts for 6% of all non-Hodgkin's lymphomas. On the genetic level, MCL is characterized by the hallmark translocation t(11;14 that is present in most cases with few exceptions. Both gene expression and comparative genomic hybridization (CGH data vary considerably between patients with implications for their prognosis. Methods We compare patients over and below the median of survival. Exploratory principal component analysis of gene expression data showed that the second principal component correlates well with patient survival. Explorative analysis of CGH data shows the same correlation. Results On chromosome 7 and 9 specific genes and bands are delineated which improve prognosis prediction independent of the previously described proliferation signature. We identify a compact survival predictor of seven genes for MCL patients. After extensive re-annotation using GEPAT, we established protein networks correlating with prognosis. Well known genes (CDC2, CCND1 and further proliferation markers (WEE1, CDC25, aurora kinases, BUB1, PCNA, E2F1 form a tight interaction network, but also non-proliferative genes (SOCS1, TUBA1B CEBPB are shown to be associated with prognosis. Furthermore we show that aggressive MCL implicates a gene network shift to higher expressed genes in late cell cycle states and refine the set of non-proliferative genes implicated with bad prognosis in MCL. Conclusion The results from explorative data analysis of gene expression and CGH data are complementary to each other. Including further tests such as Wilcoxon rank test we point both to proliferative and non-proliferative gene networks implicated in inferior prognosis of MCL and identify suitable markers both in gene expression and CGH data.

  10. The characteristic analysis and optimization design for HgCdTe TDI infrared detector array

    Science.gov (United States)

    Dong, Mei-feng; Chen, Xing; Qiu, Guang-yin; Xie, Xiao-hui

    2011-08-01

    Time Delay Integration (TDI) is an effective approach for high sensitive infrared detectors. According to the principle of the TDI, the central distance of pixel along the time delay integral direction is closely linked with the specific application requirements. So the optimization design, such as the area of pixels and their distance, plays an important role to improve the performance of TDI detectors. The crosstalk between pixels is a crucial factor that results in the decline of detector Modulation Transfer Function (MTF), and then affects the imaging quality. In this paper the optimization design rule for the arrangement of pixel has been investigated. The results show that the main method to appreciate it is reducing the crosstalk between pixels and enhancing detectivity. Chips of which pixel areas and edge intervals are different but with same distance were designed for the experiments. The optical and electrical measurements were carried out for these chips and the optimized structure was obtained. In addition, relationships between the crosstalk and parameters of material, pixel structure were analyzed based on the experiment data. According to the comprehensive analysis of the measurement data, we obtained the optimum design for specific HgCdTe TDI infrared detector. Meanwhile it is also a well reference for other HgCdTe TDI detector structure design.

  11. Design for strong absorption in a nanowire array tandem solar cell

    Science.gov (United States)

    Chen, Yang; Pistol, Mats-Erik; Anttu, Nicklas

    2016-01-01

    Semiconductor nanowires are a promising candidate for next-generation solar cells. However, the optical response of nanowires is, due to diffraction effects, complicated to optimize. Here, we optimize through optical modeling the absorption in a dual-junction nanowire-array solar cell in terms of the Shockley-Quessier detailed balance efficiency limit. We identify efficiency maxima that originate from resonant absorption of photons through the HE11 and the HE12 waveguide modes in the top cell. An efficiency limit above 40% is reached in the band gap optimized Al0.10Ga0.90As/In0.34Ga0.66As system when we allow for different diameter for the top and the bottom nanowire subcell. However, for experiments, equal diameter for the top and the bottom cell might be easier to realize. In this case, we find in our modeling a modest 1–2% drop in the efficiency limit. In the Ga0.51In0.49P/InP system, an efficiency limit of η = 37.3% could be reached. These efficiencies, which include reflection losses and sub-optimal absorption, are well above the 31.0% limit of a perfectly-absorbing, idealized single-junction bulk cell, and close to the 42.0% limit of the idealized dual-junction bulk cell. Our results offer guidance in the choice of materials and dimensions for nanowires with potential for high efficiency tandem solar cells. PMID:27574019

  12. Design of the HEAO main bus shunt regulator. [High Energy Astronomical Observatory solar array

    Science.gov (United States)

    Middlebrook, R. D.; Kimble, S. G.

    1976-01-01

    The High Energy Astronomy Observatory (HEAO) is being built for NASA by an American company. The general requirements concerning the HEAO main bus regulator are examined. The bus regulated voltage is 33 V, the maximum shunt current is 45 A, and the regulator output impedance is to be less than 0.5 Ohm from dc to 100 kHz. Loop gain design considerations for the main bus regulator are discussed and a description is given of the general device configuration. Attention is also given to regulator loop design and performance.

  13. Low concentration ratio solar array for low Earth orbit multi-100 kW application. Volume 1: Design, analysis and development tests

    Science.gov (United States)

    1983-01-01

    A preliminary design effort directed toward a low concentration ratio photovoltaic array system capable of delivering multihundred kilowatts (300 kW to 1000 kW range) in low earth orbit is described. The array system consists of two or more array modules each capable of delivering between 113 kW to 175 kW using silicon solar cells or gallium arsenide solar cells, respectively. The array module deployed area is 1320 square meters and consists of 4356 pyramidal concentrator elements. The module, when stowed in the Space Shuttle's payload bay, has a stowage volume of a cube with 3.24 meters on a side. The concentrator elements are sized for a geometric concentration ratio (GCR) of six with an aperture area of .25 sq. m. The structural analysis and design trades leading to the baseline design are discussed. It describes the configuration, as well as optical, thermal and electrical performance analyses that support the design and overall performance estimates for the array are described.

  14. Structural Design Considerations for a 50 kW-Class Solar Array for NASA's Asteroid Redirect Mission

    Science.gov (United States)

    Kerslake, Thomas W.; Kraft, Thomas G.; Yim, John T.; Le, Dzu K.

    2016-01-01

    NASA is planning an Asteroid Redirect Mission (ARM) to take place in the 2020s. To enable this multi-year mission, a 40 kW class solar electric propulsion (SEP) system powered by an advanced 50 kW class solar array will be required. Powered by the SEP module (SEPM), the ARM vehicle will travel to a large near-Earth asteroid, descend to its surface, capture a multi-metric ton (t) asteroid boulder, ascend from the surface and return to the Earth-moon system to ultimately place the ARM vehicle and its captured asteroid boulder into a stable distant orbit. During the years that follow, astronauts flying in the Orion multipurpose crew vehicle (MPCV) will dock with the ARM vehicle and conduct extra-vehicular activity (EVA) operations to explore and sample the asteroid boulder. This paper will review the top structural design considerations to successfully implement this 50 kW class solar array that must meet unprecedented performance levels. These considerations include beyond state-of-the-art metrics for specific mass, specific volume, deployed area, deployed solar array wing (SAW) keep in zone (KIZ), deployed strength and deployed frequency. Analytical and design results are presented that support definition of stowed KIZ and launch restraint interface definition. An offset boom is defined to meet the deployed SAW KIZ. The resulting parametric impact of the offset boom length on spacecraft moment of inertias and deployed SAW quasistatic and dynamic load cases are also presented. Load cases include ARM spacecraft thruster plume impingement, asteroid surface operations and Orion docking operations which drive the required SAW deployed strength and damping. The authors conclude that to support NASA's ARM power needs, an advanced SAW is required with mass performance better than 125 W/kg, stowed volume better than 40 kW/cu m, a deployed area of 200 sq m (100 sq m for each of two SAWs), a deployed SAW offset distance of nominally 3-4 m, a deployed SAW quasistatic strength

  15. Automated imitating-measuring complex for designing and measuring characteristics of phased antenna arrays

    OpenAIRE

    Usin, V.; Markov, V.; Pomazanov, S.; Usina, A.; Filonenko, A.

    2011-01-01

    This article considers design principles, structure and technical characteristics of automated imitating-measuring complex, contains variants of its hardware and software implementation for selecting APD, tolerance justification, estimation of manufacturing errors’ influence, discrete nature of control and mutual influence of radiating elements on PAA parameters.

  16. Housing Arrays Following Disasters: Social Vulnerability Considerations in Designing Transitional Communities

    Science.gov (United States)

    Spokane, Arnold R.; Mori, Yoko; Martinez, Frank

    2013-01-01

    Displacement and dislocation from homes disrupt fundamental social processes necessary for optimal community functioning. Neighborhood and community social capital, collective efficacy and place attachment are social processes that may be compromised following disaster, conflict, and upheaval. A collaborative approach to the preplanning, design,…

  17. Design, fabrication and testing of 17um pitch 640x480 uncooled infrared focal plane array detector

    Science.gov (United States)

    Jiang, Lijun; Liu, Haitao; Chi, Jiguang; Qian, Liangshan; Pan, Feng; Liu, Xiang

    2015-10-01

    Uncooled infrared focal plane array (UIRFPA) detectors are widely used in industrial thermography cameras, night vision goggles, thermal weapon sights, as well as automotive night vision systems. To meet the market requirement for smaller pixel pitch and higher resolution, we have developed a 17um pitch 640x480 UIRFPA detector. The detector is based on amorphous silicon (a-Si) microbolometer technology, the readout integrated circuit (ROIC) is designed and manufactured with 0.35um standard CMOS technology on 8 inch wafer, the microbolometer is fabricated monolithically on the ROIC using an unique surface micromachining process developed inside the company, the fabricated detector is vacuum packaged with hermetic metal package and tested. In this paper we present the design, fabrication and testing of the 17um 640x480 detector. The design trade-off of the detector ROIC and pixel micro-bridge structure will be discussed, by comparison the calculation and simulation to the testing results. The novel surface micromachining process using silicon sacrificial layer will be presented, which is more compatible with the CMOS process than the traditional process with polyimide sacrificial layer, and resulted in good processing stability and high fabrication yield. The performance of the detector is tested, with temperature equivalent temperature difference (NETD) less than 60mK at F/1 aperture, operability better than 99.5%. The results demonstrate that the detector can meet the requirements of most thermography and night vision applications.

  18. Development of an accelerated test design for predicting the service life of the solar array at Mead, Nebraska

    Energy Technology Data Exchange (ETDEWEB)

    Gaines, G.B.; Thomas, R.E.; Noel, G.T.; Shilliday, T.S.; Wood, V.E.; Carmichael, D.C.

    1979-06-07

    This report describes an accelerated test which is designed to predict the life of the 25-kW photovoltaic array installed near Mead, Nebraska. Emphasis is placed on the power-output degradation at the module level and on long-term degradation modes, as appropriate for life prediction of mature devices for which infant failures are few. A quantitative model for accelerating testing using multiple environmental stresses is used to develop the test design. The model accounts for the effects of thermal stress by a relation of the Arrhenius form. This relation is then corrected for the effects of nonthermal environmental stresses such as relative humidity, atmospheric pollutants, and ultraviolet radiation. The test conditions, measurements, and data analyses for the accelerated tests are presented for determining the predicted life of the modules in service at Mead. Constant-temperature, cyclic-temperature, and uv types of tests are specified, incorporating selected levels of relative humidity and chemical contamination and an imposed forward-bias current and static electric field. It is recommended that as a first step in test implementation, the model be selectively validated using identified portions of the accelerated test design.

  19. Design, Prototyping and Measurement of a Cascaded 6-GHz Frequency Selective Surface Array

    Directory of Open Access Journals (Sweden)

    Marcelo Bender Perotoni

    2016-04-01

    Full Text Available The design, prototyping, and free-space measurement of a 6-GHz Frequency Selective Surface filter is presented. The prototyping resolution of a large (A4 sheet size Frequency Selective Surface with small loops as elements is checked, as well as the correlation with measurements performed with a 3-D full-wave solver. The test also involved the effect of cascading two different Frequency Selective Surfaces with a viewpoint towards a narrower frequency range, which provided good results.

  20. Design of a ROIC for scanning type HgCdTe LWIR focal plane arrays

    OpenAIRE

    Yazıcı, Melik; Yazici, Melik; Kayahan, Hüseyin; Kayahan, Huseyin; Ceylan, Ömer; Ceylan, Omer; Gürbüz, Yaşar; Gurbuz, Yasar

    2010-01-01

    Design of a silicon readout integrated circuit (ROIC) for LWIR HgCdTe Focal Plane is presented. ROIC incorporates time delay integration (TDI) functionality over seven elements with a supersampling rate of three, increasing SNR and the spatial resolution. Novelty of this topology is inside TDI stage; integration of charges in TDI stage implemented in current domain by using switched current structures that reduces required area for chip and improves linearity performance. ROIC, in terms of...

  1. Experimental evaluation of the Battelle accelerated test design for the solar array at Mead, Nebraska

    Energy Technology Data Exchange (ETDEWEB)

    Frickland, P.O.; Repar, J.

    1982-04-06

    A previously developed test design for accelerated aging of photovoltaic modules was experimentally evaluated. The studies included a review of relevant field experience, environmental chamber cycling of full-size modules, and electrical and physical evaluation of the effects of accelerated aging during and after the tests. The test results indicated that thermally induced fatigue of the interconnects was the primary mode of module failure as measured by normalized power output. No chemical change in the silicone encapsulant was detectable after 360 test cycles.

  2. Design and scheduling for periodic concurrent error detection and recovery in processor arrays

    Science.gov (United States)

    Wang, Yi-Min; Chung, Pi-Yu; Fuchs, W. Kent

    1992-01-01

    Periodic application of time-redundant error checking provides the trade-off between error detection latency and performance degradation. The goal is to achieve high error coverage while satisfying performance requirements. We derive the optimal scheduling of checking patterns in order to uniformly distribute the available checking capability and maximize the error coverage. Synchronous buffering designs using data forwarding and dynamic reconfiguration are described. Efficient single-cycle diagnosis is implemented by error pattern analysis and direct-mapped recovery cache. A rollback recovery scheme using start-up control for local recovery is also presented.

  3. DNA Copy Number Aberrations in Breast Cancer by Array Comparative Genomic Hybridization

    Institute of Scientific and Technical Information of China (English)

    Jian Li; Kai Wang; Shengting Li; Vera Timmermans-Wielenga; Fritz Rank; Carsten Wiuf; Xiuqing Zhang; Huanming Yang; Lars Bolund

    2009-01-01

    Array comparative genomic hybridization (CGH) has been popularly used for an-alyzing DNA copy number variations in diseases like cancer. In this study, we investigated 82 sporadic samples from 49 breast cancer patients using 1-Mb reso-lution bacterial artificial chromosome CGH arrays. A number of highly frequent genomic aberrations were discovered, which may act as "drivers" of tumor pro-gression. Meanwhile, the genomic profiles of four "normal" breast tissue samples taken at least 2 cm away from the primary tumor sites were also found to have some genomic aberrations that recurred with high frequency in the primary tu-mors, which may have important implications for clinical therapy. Additionally, we performed class comparison and class prediction for various clinicopathological pa-rameters, and a list of characteristic genomic aberrations associated with different clinicopathological phenotypes was compiled. Our study provides clues for further investigations of the underlying mechanisms of breast carcinogenesis.

  4. A 34K SNP genotyping array for Populus trichocarpa: design, application to the study of natural populations and transferability to other Populus species

    Energy Technology Data Exchange (ETDEWEB)

    Geraldes, Armando [University of British Columbia, Vancouver; Hannemann, Jan [University of Victoria, Canada; Grassa, Chris [University of British Columbia, Vancouver; Farzaneh, Nima [University of British Columbia, Vancouver; Porth, Ilga [University of British Columbia, Vancouver; McKown, Athena [University of British Columbia, Vancouver; Skyba, Oleksandr [University of British Columbia, Vancouver; Li, Eryang [University of British Columbia, Vancouver; Mike, Fujita [University of British Columbia, Vancouver; Friedmann, Michael [University of British Columbia, Vancouver; Wasteneys, Geoffrey [University of British Columbia, Vancouver; Guy, Robert [University of British Columbia, Vancouver; El-Kassaby, Yousry [University of British Columbia, Vancouver; Mansfield, Shawn [University of British Columbia, Vancouver; Cronk, Quentin [University of British Columbia, Vancouver; Ehlting, Juergen [University of Victoria, Canada; Douglas, Carl [University of British Columbia, Vancouver; DiFazio, Stephen P [West Virginia University, Morgantown; Slavov, Gancho [West Virginia University, Morgantown; Ranjan, Priya [ORNL; Muchero, Wellington [ORNL; Gunter, Lee E [ORNL; Wymore, Ann [ORNL; Tuskan, Gerald A [ORNL; Martin, Joel [U.S. Department of Energy, Joint Genome Institute; Schackwitz, Wendy [U.S. Department of Energy, Joint Genome Institute; Pennacchio, Christa [U.S. Department of Energy, Joint Genome Institute; Rokhsar, Daniel [U.S. Department of Energy, Joint Genome Institute

    2013-01-01

    Genetic mapping of quantitative traits requires genotypic data for large numbers of markers in many individuals. Despite the declining costs of genotyping by sequencing, for most studies, the use of large SNP genotyping arrays still offers the most cost-effective solution for large-scale targeted genotyping. Here we report on the design and performance of a SNP genotyping array for Populus trichocarpa (black cottonwood). This genotyping array was designed with SNPs pre-ascertained in 34 wild accessions covering most of the species range. Due to the rapid decay of linkage disequilibrium in P. trichocarpa we adopted a candidate gene approach to the array design that resulted in the selection of 34,131 SNPs, the majority of which are located in, or within 2 kb, of 3,543 candidate genes. A subset of the SNPs (539) was selected based on patterns of variation among the SNP discovery accessions. We show that more than 95% of the loci produce high quality genotypes and that the genotyping error rate for these is likely below 2%, indicating that high-quality data are generated with this array. We demonstrate that even among small numbers of samples (n=10) from local populations over 84% of loci are polymorphic. We also tested the applicability of the array to other species in the genus and found that due to ascertainment bias the number of polymorphic loci decreases rapidly with genetic distance, with the largest numbers detected in other species in section Tacamahaca (P. balsamifera and P. angustifolia). Finally, we provide evidence for the utility of the array for intraspecific studies of genetic differentiation and for species assignment and the detection of natural hybrids.

  5. Somatic mosaicism detected by exon-targeted, high-resolution aCGH in 10 362 consecutive cases

    Science.gov (United States)

    Pham, Justin; Shaw, Chad; Pursley, Amber; Hixson, Patricia; Sampath, Srirangan; Roney, Erin; Gambin, Tomasz; Kang, Sung-Hae L; Bi, Weimin; Lalani, Seema; Bacino, Carlos; Lupski, James R; Stankiewicz, Pawel; Patel, Ankita; Cheung, Sau-Wai

    2014-01-01

    Somatic chromosomal mosaicism arising from post-zygotic errors is known to cause several well-defined genetic syndromes as well as contribute to phenotypic variation in diseases. However, somatic mosaicism is often under-diagnosed due to challenges in detection. We evaluated 10 362 patients with a custom-designed, exon-targeted whole-genome oligonucleotide array and detected somatic mosaicism in a total of 57 cases (0.55%). The mosaicism was characterized and confirmed by fluorescence in situ hybridization (FISH) and/or chromosome analysis. Different categories of abnormal cell lines were detected: (1) aneuploidy, including sex chromosome abnormalities and isochromosomes (22 cases), (2) ring or marker chromosomes (12 cases), (3) single deletion/duplication copy number variations (CNVs) (11 cases), (4) multiple deletion/duplication CNVs (5 cases), (5) exonic CNVs (4 cases), and (6) unbalanced translocations (3 cases). Levels of mosaicism calculated based on the array data were in good concordance with those observed by FISH (10–93%). Of the 14 cases evaluated concurrently by chromosome analysis, mosaicism was detected solely by the array in 4 cases (29%). In summary, our exon-targeted array further expands the diagnostic capability of high-resolution array comparative genomic hybridization in detecting mosaicism for cytogenetic abnormalities as well as small CNVs in disease-causing genes. PMID:24398791

  6. Somatic mosaicism detected by exon-targeted, high-resolution aCGH in 10,362 consecutive cases.

    Science.gov (United States)

    Pham, Justin; Shaw, Chad; Pursley, Amber; Hixson, Patricia; Sampath, Srirangan; Roney, Erin; Gambin, Tomasz; Kang, Sung-Hae L; Bi, Weimin; Lalani, Seema; Bacino, Carlos; Lupski, James R; Stankiewicz, Pawel; Patel, Ankita; Cheung, Sau-Wai

    2014-08-01

    Somatic chromosomal mosaicism arising from post-zygotic errors is known to cause several well-defined genetic syndromes as well as contribute to phenotypic variation in diseases. However, somatic mosaicism is often under-diagnosed due to challenges in detection. We evaluated 10,362 patients with a custom-designed, exon-targeted whole-genome oligonucleotide array and detected somatic mosaicism in a total of 57 cases (0.55%). The mosaicism was characterized and confirmed by fluorescence in situ hybridization (FISH) and/or chromosome analysis. Different categories of abnormal cell lines were detected: (1) aneuploidy, including sex chromosome abnormalities and isochromosomes (22 cases), (2) ring or marker chromosomes (12 cases), (3) single deletion/duplication copy number variations (CNVs) (11 cases), (4) multiple deletion/duplication CNVs (5 cases), (5) exonic CNVs (4 cases), and (6) unbalanced translocations (3 cases). Levels of mosaicism calculated based on the array data were in good concordance with those observed by FISH (10-93%). Of the 14 cases evaluated concurrently by chromosome analysis, mosaicism was detected solely by the array in 4 cases (29%). In summary, our exon-targeted array further expands the diagnostic capability of high-resolution array comparative genomic hybridization in detecting mosaicism for cytogenetic abnormalities as well as small CNVs in disease-causing genes.

  7. Design, fabrication and characterization of an arrayable all-polymer microfluidic valve employing highly magnetic rare-earth composite polymer

    Science.gov (United States)

    Rahbar, Mona; Shannon, Lesley; Gray, Bonnie L.

    2016-05-01

    We present a new magnetically actuated microfluidic valve that employs a highly magnetic composite polymer (M-CP) containing rare-earth hard-magnetic powder for its actuating element and for its valve seat. The M-CP offers much higher magnetization compared to the soft-magnetic, ferrite-based composite polymers typically used in microfluidic applications. Each valve consists of a permanently magnetized M-CP flap and valve seat mounted on a microfluidic channel system fabricated in poly(dimethylsiloxane) (PDMS). Each valve is actuated under a relatively small external magnetic field of 80 mT provided by a small permanent magnet mounted on a miniature linear actuator. The performance of the valve with different flap thicknesses is characterized. In addition, the effect of the magnetic valve seat on the valve’s performance is also characterized. It is experimentally shown that a valve with a 2.3 mm flap thickness, actuated under an 80 mT magnetic field, is capable of completely blocking liquid flow at a flow rate of 1 ml min‑1 for pressures up to 9.65 kPa in microfluidic channels 200 μm wide and 200 μm deep. The valve can also be fabricated into an array for flow switching between multiple microfluidic channels under continuous flow conditions. The performance of arrays of valves for flow routing is demonstrated for flow rates up to 5 ml min‑1 with larger microfluidic channels of up to 1 mm wide and 500 μm deep. The design of the valves is compatible with other commonly used polymeric microfluidic components, as well as other components that use the same novel permanently magnetic composite polymer, such as our previously reported cilia-based mixing devices.

  8. Design of thin-film filters for resolution improvements in filter-array based spectrometers using DSP

    Science.gov (United States)

    Lee, Woong-Bi; Kim, Cheolsun; Ju, Gun Wu; Lee, Yong Tak; Lee, Heung-No

    2016-05-01

    Miniature spectrometers have been widely developed in various academic and industrial applications such as bio-medical, chemical and environmental engineering. As a family of spectrometers, optical filter-array based spectrometers fabricated using CMOS or Nano technology provide miniaturization, superior portability and cost effectiveness. In filterarray based spectrometers, the resolution which represents the ability how closely resolve two neighboring spectra, depends on the number of filters and the characteristics of the transmission functions (TFs) of the filters. In practice, due to the small-size and low-cost fabrication, the number of filters is limited and the shape of the TF of each filter is nonideal. As a development of modern digital signal processing (DSP), the spectrometers are equipped with DSP algorithms not only to alleviate distortions due to unexpected noise or interferences among filters but also reconstruct the original signal spectrum. For a high-resolution spectrum reconstruction by the DSP, the TFs of the filters need to be sufficiently uncorrelated with each other. In this paper, we present a design of optical thin-film filters which have the uncorrelated TFs. Each filter consists of multiple layers of high- and low-refractive index materials deposited on a substrate. The proposed design helps the DSP algorithm to improve resolution with a small number of filters. We demonstrate that a resolution of 5 nm within a range from 500 nm to 1100 nm can be achieved with only 64 filters.

  9. DESIGN AND IMPLEMENTATION OF GLOBAL POSITIONING SYSTEM RECEIVER IN FIELD PROGRAMMABLE GATE ARRAY WITH SHORT MESSAGE SERVICE

    Directory of Open Access Journals (Sweden)

    Govindaraju Kavya

    2014-01-01

    Full Text Available In recent years, survival rate from myocardial infarction and bundle branch block has been increased due to the advancements in medical field. Continuous monitoring and location management of these patients becomes an essential task. Hence this study aims in design and development of GPS in a Field Programmable Gate Array (FPGA and interfaced with a GSM modem to transmit the location of a civilian or a patient to the mobile of the caretaker .The digital blocks like code acquisition, carrier tracking, code tracking and data processing are modeled in VHDL and validation of the blocks were done using MODELSIM simulator. The analog front end of the GPS receiver have been designed and integrated with Altera DE1 FPGA board which has software defined digital baseband GPS receiver. Quartus II software is used to integrate the analog front end and digital back end of the GPS receiver. The GPS output from the FPGA is given to the GSM that transmits the location to a mobile. The proposed implementation has the advantage that various blocks of the processor can be reconfigured to support multiple signal processing tasks and also can be retargeted to any family of FPGA device.

  10. Design of multichord Hα detector arrays for the U-3M torsatron and identification of rotating plasma perturbations

    Science.gov (United States)

    Dreval, M. B.; Shapoval, A. M.; Ozherelyev, F. I.; Makhov, M. M.

    2016-07-01

    An Hα camera has been designed and installed in the U-3M torsatron for spatially and temporally resolved measurements. This device provides fast measurements of the emission brightness profile in the noisy environment of the radio frequency (RF) heated plasma. Unusual topology of diagnostics and the data acquisition system are applied. All the system components, including digitizers, are assembled in a single unit. It allows the suppression of a low-frequency electromagnetic interference by eliminating the ground loops. And the suppression of RF noises is achieved by eliminating the signal interface cables and digital interface cables in the design. The Wi-Fi interface is used to prevent a ground loop in the data transfer stage. The achieved sensitivity of our diagnostics is high enough for measuring the Hα emission from the low-density (ne ≈ (1-2)ṡ1010 cm-3) plasma with a temporal resolution of about 20 μs in the noisy environment. Different types of Hα emission fluctuations within the frequency range of 1-5 kHz and poloidal mode numbers m = 0 and m = 5 have been observed in U-3M. A simple technique of the line-of-sight data analysis, based on the U-3M magnetic surface asymmetry, is proposed and used for the spatial localization of the rotating mode and for the determination of mode numbers and its poloidal rotation direction using a single Hα array.

  11. Evolving serodiagnostics by rationally designed peptide arrays: the Burkholderia paradigm in Cystic Fibrosis.

    Science.gov (United States)

    Peri, Claudio; Gori, Alessandro; Gagni, Paola; Sola, Laura; Girelli, Daniela; Sottotetti, Samantha; Cariani, Lisa; Chiari, Marcella; Cretich, Marina; Colombo, Giorgio

    2016-01-01

    Efficient diagnosis of emerging and novel bacterial infections is fundamental to guide decisions on therapeutic treatments. Here, we engineered a novel rational strategy to design peptide microarray platforms, which combines structural and genomic analyses to predict the binding interfaces between diverse protein antigens and antibodies against Burkholderia cepacia complex infections present in the sera of Cystic Fibrosis (CF) patients. The predicted binding interfaces on the antigens are synthesized in the form of isolated peptides and chemically optimized for controlled orientation on the surface. Our platform displays multiple Burkholderia-related epitopes and is shown to diagnose infected individuals even in presence of superinfections caused by other prevalent CF pathogens, with limited cost and time requirements. Moreover, our data point out that the specific patterns determined by combined probe responses might provide a characterization of Burkholderia infections even at the subtype level (genomovars). The method is general and immediately applicable to other bacteria.

  12. A new method for the design of slot antenna arrays: Theory and experiment

    KAUST Repository

    Clauzier, Sebastien

    2016-04-10

    The present paper proposes and validates a new general design methodology that can be used to automatically find proper positions and orientations of waveguide-based radiating slots capable of realizing any given radiation beam profile. The new technique combines basic radiation theory and waveguide propagation theory in a novel analytical model that allows the prediction of the radiation characteristics of generic slots without the need to perform full-wave numerical solution. The analytical model is then used to implement a low-cost objective function within a global optimization scheme (here genetic algorithm.) The algorithm is then deployed to find optimum positions and orientations of clusters of radiating slots cut into the waveguide surface such that any desired beam pattern can be obtained. The method is verified using both full-wave numerical solution and experiment.

  13. Evolving serodiagnostics by rationally designed peptide arrays: the Burkholderia paradigm in Cystic Fibrosis

    Science.gov (United States)

    Peri, Claudio; Gori, Alessandro; Gagni, Paola; Sola, Laura; Girelli, Daniela; Sottotetti, Samantha; Cariani, Lisa; Chiari, Marcella; Cretich, Marina; Colombo, Giorgio

    2016-09-01

    Efficient diagnosis of emerging and novel bacterial infections is fundamental to guide decisions on therapeutic treatments. Here, we engineered a novel rational strategy to design peptide microarray platforms, which combines structural and genomic analyses to predict the binding interfaces between diverse protein antigens and antibodies against Burkholderia cepacia complex infections present in the sera of Cystic Fibrosis (CF) patients. The predicted binding interfaces on the antigens are synthesized in the form of isolated peptides and chemically optimized for controlled orientation on the surface. Our platform displays multiple Burkholderia-related epitopes and is shown to diagnose infected individuals even in presence of superinfections caused by other prevalent CF pathogens, with limited cost and time requirements. Moreover, our data point out that the specific patterns determined by combined probe responses might provide a characterization of Burkholderia infections even at the subtype level (genomovars). The method is general and immediately applicable to other bacteria.

  14. Design of a muon tomography system with a plastic scintillator and wavelength-shifting fiber arrays

    Energy Technology Data Exchange (ETDEWEB)

    Jo, Woo Jin; Kim, Hyun-Il; An, Su Jung; Lee, Chae Young [Department of Radiological Science, College of Health Science, Yonsei University, Wonju 220-710 (Korea, Republic of); Baek, Cheol-Ha [Department of Radiological Science, Dongseo University, Busan 617-716 (Korea, Republic of); Chung, Yong Hyun, E-mail: ychung@yonsei.ac.kr [Department of Radiological Science, College of Health Science, Yonsei University, Wonju 220-710 (Korea, Republic of)

    2013-12-21

    Recently, monitoring nuclear materials to avoid nuclear terrorism has become an important area of national security. It can be difficult to detect gamma rays from nuclear material because they are easily shielded by shielding material. Muon tomography using multiple -Coulomb scattering derived from muons can be utilized to detect special nuclear materials (SNMs) such as uranium-235 and plutonium-239. We designed a muon tomography system composed of four detector modules. The incident and scattered muon tracks can be calculated by two top and two bottom detectors, respectively. 3D tomographic images are obtained by extracting the crossing points of muon tracks with a point-of-closest-approach algorithm. The purpose of this study was to optimize the muon tomography system using Monte Carlo simulation code. The effects of the geometric parameters of the muon tomography system on material Z-discrimination capability were simulated and evaluated.

  15. Field Programmable Gate Array Based Parallel Strapdown Algorithm Design for Strapdown Inertial Navigation Systems

    Directory of Open Access Journals (Sweden)

    Long-Hua Ma

    2011-08-01

    Full Text Available A new generalized optimum strapdown algorithm with coning and sculling compensation is presented, in which the position, velocity and attitude updating operations are carried out based on the single-speed structure in which all computations are executed at a single updating rate that is sufficiently high to accurately account for high frequency angular rate and acceleration rectification effects. Different from existing algorithms, the updating rates of the coning and sculling compensations are unrelated with the number of the gyro incremental angle samples and the number of the accelerometer incremental velocity samples. When the output sampling rate of inertial sensors remains constant, this algorithm allows increasing the updating rate of the coning and sculling compensation, yet with more numbers of gyro incremental angle and accelerometer incremental velocity in order to improve the accuracy of system. Then, in order to implement the new strapdown algorithm in a single FPGA chip, the parallelization of the algorithm is designed and its computational complexity is analyzed. The performance of the proposed parallel strapdown algorithm is tested on the Xilinx ISE 12.3 software platform and the FPGA device XC6VLX550T hardware platform on the basis of some fighter data. It is shown that this parallel strapdown algorithm on the FPGA platform can greatly decrease the execution time of algorithm to meet the real-time and high precision requirements of system on the high dynamic environment, relative to the existing implemented on the DSP platform.

  16. Design and Implementation of Co-Array Fortran Compiler Based on Software Shared Memory%基于软件共享存储的Co-Array Fortran编译器实现

    Institute of Scientific and Technical Information of China (English)

    黄春

    2012-01-01

    Co-Array Fortran (CAF) has become part of Fortran Programming Language Standard,and has been widely accepted in scientific computing community. The paper presented the design and implementation of a CAF Compiler based on software shared memory. Several techniques were presented to improve the performance of CAF programs,including Co-array data communication by direct array assignment, Data Padding for raising locality and reducing false share. The benchmark testing shows that the performance of CAF programs is similar to that of MPI programs, with our CAF compiler.%Co-Array Fortran(CAF)已经成为Fortran语言标准的一部分,在科学计算领域逐渐被接受.基于软件共享存储实现了一个CAF编译器,其通过直接的数组赋值实现Co-array数据通信,利用数据垫塞技术提高数据局部性,减少伪共享,优化CAF程序性能.典型科学计算程序测试表明,CAF能够获得和MPI相当的性能.

  17. Solar array deployment mechanism

    Science.gov (United States)

    Calassa, Mark C.; Kackley, Russell

    1995-05-01

    This paper describes a Solar Array Deployment Mechanism (SADM) used to deploy a rigid solar array panel on a commercial spacecraft. The application required a deployment mechanism design that was not only lightweight, but also could be produced and installed at the lowest possible cost. This paper covers design, test, and analysis of a mechanism that meets these requirements.

  18. Single Event Analysis and Fault Injection Techniques Targeting Complex Designs Implemented in Xilinx-Virtex Family Field Programmable Gate Array (FPGA) Devices

    Science.gov (United States)

    Berg, Melanie D.; Label, Kenneth; Kim, Kim

    2014-01-01

    An informative session regarding SRAM FPGA basics. Presenting a framework for fault injection techniques applied to Xilinx Field Programmable Gate Arrays (FPGAs). Introduce an overlooked time component that illustrates fault injection is impractical for most real designs as a stand-alone characterization tool. Demonstrate procedures that benefit from fault injection error analysis.

  19. Report: Optimization study of the preparation factors for argan oil microcapsule based on hybrid-level orthogonal array design via SPSS modeling.

    Science.gov (United States)

    Zhao, Xi; Wu, Xiaoli; Zhou, Hui; Jiang, Tao; Chen, Chun; Liu, Mingshi; Jin, Yuanbao; Yang, Dongsheng

    2014-11-01

    To optimize the preparation factors for argan oil microcapsule using complex coacervation of chitosan cross-linked with gelatin based on hybrid-level orthogonal array design via SPSS modeling. Eight relatively significant factors were firstly investigated and selected as calculative factors for the orthogonal array design from the total of ten factors effecting the preparation of argan oil microcapsule by utilizing the single factor variable method. The modeling of hybrid-level orthogonal array design was built in these eight factors with the relevant levels (9, 9, 9, 9, 7, 6, 2 and 2 respectively). The preparation factors for argan oil microcapsule were investigated and optimized according to the results of hybrid-level orthogonal array design. The priorities order and relevant optimum levels of preparation factors standard to base on the percentage of microcapsule with the diameter of 30~40 μm via SPSS. Experimental data showed that the optimum factors were controlling the chitosan/gelatin ratio, the systemic concentration and the core/shell ratio at 1:2, 1.5% and 1:7 respectively, presetting complex coacervation pH at 6.4, setting cross-linking time and complex coacervation at 75 min and 30 min, using the glucose-delta lactone as the type of cross-linking agent, and selecting chitosan with the molecular weight of 2000~3000.

  20. Design of Vivaldi antenna array with end-fire beam steering function for 5G mobile terminals

    DEFF Research Database (Denmark)

    Ojaroudiparchin, Naser; Shen, Ming; Pedersen, Gert F.

    2015-01-01

    to form a linear phased array in the edge region (top-side) on a mobile phone PCB. The simulated results show that the antenna has the reflection coefficient (S11) less than -10 dB in the frequency range of 27.4 to 28.6 GHz. The proposed phased array antenna has good gain, efficiency, and 3D beam steering...

  1. Concept and design of a genome-wide association genotyping array tailored for transplantation-specific studies

    DEFF Research Database (Denmark)

    Li, Yun R.; van Setten, Jessica; Verma, Shefali S.;

    2015-01-01

    genome-wide genotyping array, the 'TxArray', comprising approximately 782,000 markers with tailored content for deeper capture of variants across HLA, KIR, pharmacogenomic, and metabolic loci important in transplantation. To test concordance and genotyping quality, we genotyped 85 HapMap samples...

  2. Investigation of the Design Boundaries of a 3,072 X 2,048 Image Sensor Pixel Array

    Science.gov (United States)

    Eid, El-Sayed I.

    2002-09-01

    The practical boundaries surrounding the design of very high resolution image sensors have been studied. The case study used to analyze these practical boundaries is a CMOS photodiode active pixel sensor (APS) image sensor with pixel array format of 3,072 (H) X 2,048 (V). The frame rate of the image sensor is variable up to 30 frames per second (fps), leading to a maximum image data throughput of 180 M pixels per second. The pixel size is 6.0 im, resulting in a pixel fill factor of approximately 48% (implemented in a 0.25 μm CMOS fabrication process) and a 4/3 inch optical format. The resultant die fill factor is approximately 54%. The column-parallel approach, which works well for both the on-chip analog signal processing and analog-to-digital conversion, is adopted. The 10-bit successive approximation ADC was deemed suitable for on-chip integration. The projected total power consumption of the case study image sensor chip is below 200 mW at 3.3-V power supply and below 100 mW at 1.5-V power supply. These power estimates were made for operation at full resolution (6 M pixels per frame) and at maximum frame rate (30 fps), leading to a maximum digital image data throughput of 1.8 G bits per second.

  3. Design and Imaging Application of Room-Temperature Terahertz Detector with Micro-Bolometer Focal Plane Array

    Institute of Scientific and Technical Information of China (English)

    Jun Wang; Jun Gou; Zhi-Ming Wu; Ya-Dong Jiang

    2016-01-01

    Abstract'Room-temperature terahertz (THz) detectors indicate a great potential in the imaging application because of their real-time, compact bulk, and wide spectral band responding characteristics. THz detectors with different dimensions based on a micro-bridge structure have been designed and fabricated to get optimized micro-bolometer parameters from the test results of membrane deformation. A nanostructured titanium (Ti) thin film absorber is integrated in the micro-bridge structure of the VOx micro-bolometer by a combined process of magnetron sputtering and reactive ion etching (RIE), and its improvement of THz absorption is verified by an optical characteristics mesurement. Continuous-wave THz detection and imaging are demonstrated by using a 2.52 THz far infrared CO2 laser and a 320×240 vanadium oxide micro-bolometer focal plane array with an optimized cell structure. With this detecting system, THz imaging of metal concealed in a wiping cloth and an envelope is demonstrated, respectively.

  4. Design, Fabrication and Characterization of a Low-Impedance 3D Electrode Array System for Neuro-Electrophysiology

    Directory of Open Access Journals (Sweden)

    Mihaela Kusko

    2012-12-01

    Full Text Available Recent progress in patterned microelectrode manufacturing technology and microfluidics has opened the way to a large variety of cellular and molecular biosensor-based applications. In this extremely diverse and rapidly expanding landscape, silicon-based technologies occupy a special position, given their statute of mature, consolidated, and highly accessible areas of development. Within the present work we report microfabrication procedures and workflows for 3D patterned gold-plated microelectrode arrays (MEA of different shapes (pyramidal, conical and high aspect ratio, and we provide a detailed characterization of their physical features during all the fabrication steps to have in the end a reliable technology. Moreover, the electrical performances of MEA silicon chips mounted on standardized connector boards via ultrasound wire-bonding have been tested using non-destructive electrochemical methods: linear sweep and cyclic voltammetry, impedance spectroscopy. Further, an experimental recording chamber package suitable for in vitro electrophysiology experiments has been realized using custom-design electronics for electrical stimulus delivery and local field potential recording, included in a complete electrophysiology setup, and the experimental structures have been tested on newborn rat hippocampal slices, yielding similar performance compared to commercially available MEA equipments.

  5. System-on-chip field-programmable gate array design for onboard real-time hyperspectral unmixing

    Science.gov (United States)

    Nascimento, José M. P.; Véstias, Mário

    2016-01-01

    Hyperspectral instruments have been incorporated in satellite missions, providing large amounts of data of high spectral resolution of the Earth surface. This data can be used in remote sensing applications that often require a real-time or near-real-time response. To avoid delays between hyperspectral image acquisition and its interpretation, the last usually done on a ground station, onboard systems have emerged to process data, reducing the volume of information to transfer from the satellite to the ground station. For this purpose, compact reconfigurable hardware modules, such as field-programmable gate arrays (FPGAs), are widely used. This paper proposes an FPGA-based architecture for hyperspectral unmixing. This method based on the vertex component analysis (VCA) and it works without a dimensionality reduction preprocessing step. The architecture has been designed for a low-cost Xilinx Zynq board with a Zynq-7020 system-on-chip FPGA-based on the Artix-7 FPGA programmable logic and tested using real hyperspectral data. Experimental results indicate that the proposed implementation can achieve real-time processing, while maintaining the methods accuracy, which indicate the potential of the proposed platform to implement high-performance, low-cost embedded systems, opening perspectives for onboard hyperspectral image processing.

  6. A MIMO Reversed Antenna Array Design for gsm1800/td-scdma/lte/wi-max/wiLan/wifi

    Directory of Open Access Journals (Sweden)

    Fang Xu

    2015-01-01

    Full Text Available A triband MIMO antenna used for gsm1800/td-scdma/lte/wi-max/wiLan/wifi has been proposed in this paper. The design with the size of 32 m ∗ 73.6 mm is fabricated on FR4 substrate (thickness is 1.6 mm. This antenna consists of two reversed monopole meandered radiators and defected ground structure which work together to achieve impedance match and enhance isolation for all operating bands. According to the subsequent measured results, the working frequency domains are at gsm1800 (1.71 GHz–1.88 GHz, td-scdma (1.88 GHz–2.025 GHz, lte (2.1–2.35, 2.48–2.51, wi-max (3.5 GHz, 5.5 GHz, wiLan (5.725 GHz–5.8 GHz, and wifi (5.8 GHz–5.88 GHz approximately. Besides, the measured radiation patterns (gain total are also well at these working frequency domains and the omnidirectional performance has been achieved for the reversed MIMO antenna array. Furthermore, the realized isolation is more than 15 dB, 24.2 dB, and 22 dB at the first, the second, and the third working frequency band, respectively.

  7. Increased nuchal translucency with normal karyotype: a follow-up study of 100 cases supplemented with CGH and MLPA analyses

    DEFF Research Database (Denmark)

    Schou, K V; Kirchhoff, M; Nygaard, U;

    2009-01-01

    OBJECTIVE: To evaluate whether high-resolution comparative genomic hybridization (HR-CGH) and subtelomeric and syndrome-specific multiplex ligation-dependent probe amplification (MLPA) would detect minor chromosomal aberrations in fetuses with increased nuchal translucency thickness (NT) and norm...... disease, which supports the current approach of repeated ultrasound examinations in these high-risk pregnancies....... and subtelomeric regions. Pregnancy outcome was followed up. RESULTS: Among 80 liveborn children who were followed up, three (4%) had syndromes involving mental retardation, including a case of Sotos syndrome caused by a de novo mutation. 15% of fetuses were lost during pregnancy due to abnormalities...

  8. Design and fabrication of a flexible MEMS-based electro-mechanical sensor array for breast cancer diagnosis

    Science.gov (United States)

    Pandya, Hardik J.; Park, Kihan; Desai, Jaydev P.

    2015-07-01

    The use of flexible micro-electro-mechanical systems (MEMS)-based devices provides a unique opportunity in bio-medical robotics such as the characterization of normal and malignant tissues. This paper reports on the design and development of a flexible MEMS-based sensor array integrating mechanical and electrical sensors on the same platform to enable the study of the change in electro-mechanical properties of benign and cancerous breast tissues. In this work, we present the analysis of the electrical characterization of the tissue specimens and also demonstrate the feasibility of using the sensor for the mechanical characterization of tissue specimens. Eight strain gauges acting as mechanical sensors were fabricated using poly(3,4-ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS) conducting polymer on poly(dimethylsiloxane) (PDMS) as the substrate material. Eight electrical sensors were fabricated using SU-8 pillars on gold (Au) pads which were patterned on the strain gauges separated by a thin insulator (SiO2 1.0 μm). These pillars were coated with gold to make them conducting. The electro-mechanical sensors are integrated on the same substrate. The sensor array covers a 180 μm  ×  180 μm area and the size of the complete device is 20 mm in diameter. The diameter of each breast tissue core used in the present study was 1 mm and the thickness was 8 μm. The region of interest was 200 μm  ×  200 μm. A microindentation technique was used to characterize the mechanical properties of the breast tissues. The sensor is integrated with conducting SU-8 pillars to study the electrical property of the tissue. Through electro-mechanical characterization studies using this MEMS-based sensor, we were able to measure the accuracy of the fabricated device and ascertain the difference between benign and cancers breast tissue specimens.

  9. Design and fabrication of a flexible MEMS-based electro-mechanical sensor array for breast cancer diagnosis

    International Nuclear Information System (INIS)

    The use of flexible micro-electro-mechanical systems (MEMS)-based devices provides a unique opportunity in bio-medical robotics such as the characterization of normal and malignant tissues. This paper reports on the design and development of a flexible MEMS-based sensor array integrating mechanical and electrical sensors on the same platform to enable the study of the change in electro-mechanical properties of benign and cancerous breast tissues. In this work, we present the analysis of the electrical characterization of the tissue specimens and also demonstrate the feasibility of using the sensor for the mechanical characterization of tissue specimens. Eight strain gauges acting as mechanical sensors were fabricated using poly(3,4-ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS) conducting polymer on poly(dimethylsiloxane) (PDMS) as the substrate material. Eight electrical sensors were fabricated using SU-8 pillars on gold (Au) pads which were patterned on the strain gauges separated by a thin insulator (SiO2 1.0 μm). These pillars were coated with gold to make them conducting. The electro-mechanical sensors are integrated on the same substrate. The sensor array covers a 180 μm  ×  180 μm area and the size of the complete device is 20 mm in diameter. The diameter of each breast tissue core used in the present study was 1 mm and the thickness was 8 μm. The region of interest was 200 μm  ×  200 μm. A microindentation technique was used to characterize the mechanical properties of the breast tissues. The sensor is integrated with conducting SU-8 pillars to study the electrical property of the tissue. Through electro-mechanical characterization studies using this MEMS-based sensor, we were able to measure the accuracy of the fabricated device and ascertain the difference between benign and cancers breast tissue specimens. (paper)

  10. 利用Fine-tiling aCGH分析TCR基因重排鉴定T细胞白血病克隆%Analysis of TCR gene rearrangement for identification of T cell leukemia clone by using Fine-tiling aCGH

    Institute of Scientific and Technical Information of China (English)

    郑海涛; 叶铁真; 陈少华; 杨力建; 卢育洪; 李扬秋

    2011-01-01

    AIM: To establish a new method which analyzes T cell receptor (TCR) gene rearrangement for identification of T cells acute lymphoblastic leukemia (T-ALL)clone, it will provide the basis for the study of T-ALL including the chromosome translocation involving TCR loci.METHODS: Total DNA was isolated from peripheral blood mononuclear cells (PBMC) of one case with T-ALL. Using the fine-tiling array comparative genomic hybridization (finetiling aCGH) to analyze the genomic DNA differences of the case and control group, we could find the breakpoints and their position in the chromosomes. According to the preliminary results, we could design the specific primers for the positions of the breakpoints relative to sequence. Furthermore, the ligation-mediated PCR (LM-PCR) and sequence analysis were used to identify the TCR gene rearrangement.And TCR gene expression was detected by RT-PCR. RESULTS: The fine-tiling aCGH results of the T-ALL showed that the TCRα/δ locus of chromosome 14 appeared four breakpoints, corresponding to TCR Vδ1, Vδ2, Jδl and Jδ2.By LM-PCR, sequencing and sequence analysis, TCR gene of the case of T-ALL was involved in Vδ1Dδ2Dδ3Jδl,Vδ2Dδ3Jδ2 rearrangement. RT-PCR results also confirmed the expression of these TCR gene rearrangements. CONCLUSION: The results demonstrated that fine-tiling aCGH and LM-PCR techniques could be used to identify the TCR gene rearrangement as one of the best perfect methods.And it was also a way to find some fusion genes involving in TCR gene.%目的:建立基于分析T细胞受体(TCR)基因重排而确定T细胞-急性淋巴细胞白血病(T-ALL)克隆的新方法,为研究T-ALL中涉及TCR基因位点的染色体易位提供基础.方法:提取1例T-ALL患者外周血单个核细胞(PBMC)的总DNA,利用精细定位的寡核苷酸阵列比较基因组杂交(fine-tiling aCGH)分析样本与对照组基因组DNA的差异,了解不同染色体上可能的断裂点和具体的位点,根据所提供的初步结果,

  11. Experimental and numerical analysis of B1+ field and SAR with a new transmit array design for 7 T breast MRI

    Science.gov (United States)

    Kim, Junghwan; Krishnamurthy, Narayan; Santini, Tales; Zhao, Yujuan; Zhao, Tiejun; Bae, Kyongtae Ty; Ibrahim, Tamer S.

    2016-08-01

    Developing a radiofrequency (RF) coil system that produces a uniform B1+ field (circularly polarized component of the transverse magnetic field responsible for excitation) and low specific absorption rate (SAR) is critical for high performance ultrahigh field human imaging. In this study, we provide the design of a new eight channel radiofrequency (RF) transmit (Tx) array for breast MRI at 7 T. A numerical analysis utilizing an in-house finite difference time domain (FDTD) package was carried out in (1) four breast models, (2) homogeneous spherical model and (3) full body model to calculate the B1+ intensity (μT) and homogeneity represented by coefficient of variation (CoV = standard deviation/mean) in the proposed RF array design. The numerical results were compared with that measured in breast phantom (Bphantom) and homogeneous spherical phantom at 7 T MRI and showed very good agreement. Average and peak SARs were also calculated in the four breast models and the temperature rises due to the operation of the RF array were also measured in the Bphantom. The proposed RF array; which can operate in a single or multi transmit modes, demonstrates homogeneous RF field excitation with acceptable local/average SAR levels for breast MRI at 7 T.

  12. Design and Application of Wuhan Ionospheric Oblique Backscattering Sounding System with the Addition of an Antenna Array (WIOBSS-AA)

    Science.gov (United States)

    Cui, Xiao; Chen, Gang; Wang, Jin; Song, Huan; Gong, Wanlin

    2016-01-01

    The Wuhan Ionospheric Oblique Backscattering Sounding System with the addition of an antenna array (WIOBSS-AA) is the newest member of the WIOBSS family. It is a multi-channel radio system using phased-array antenna technology. The transmitting part of this radio system applies an array composed of five log-periodic antennas to form five beams that span an area to the northwest of the radar site. The hardware and the antenna array of the first multi-channel ionosonde in the WIOBSS family are introduced in detail in this paper. An ionospheric detection experiment was carried out in Chongyang, Hubei province, China on 16 March 2015 to examine the performance of WIOBSS-AA. The radio system demonstrated its ability to obtain ionospheric electron density information over a wide area. The observations indicate that during the experiment, the monitored large-area ionospheric F2-layer was calm and electron density increased with decreasing latitude. PMID:27314360

  13. Design and Application of Wuhan Ionospheric Oblique Backscattering Sounding System with the Addition of an Antenna Array (WIOBSS-AA).

    Science.gov (United States)

    Cui, Xiao; Chen, Gang; Wang, Jin; Song, Huan; Gong, Wanlin

    2016-01-01

    The Wuhan Ionospheric Oblique Backscattering Sounding System with the addition of an antenna array (WIOBSS-AA) is the newest member of the WIOBSS family. It is a multi-channel radio system using phased-array antenna technology. The transmitting part of this radio system applies an array composed of five log-periodic antennas to form five beams that span an area to the northwest of the radar site. The hardware and the antenna array of the first multi-channel ionosonde in the WIOBSS family are introduced in detail in this paper. An ionospheric detection experiment was carried out in Chongyang, Hubei province, China on 16 March 2015 to examine the performance of WIOBSS-AA. The radio system demonstrated its ability to obtain ionospheric electron density information over a wide area. The observations indicate that during the experiment, the monitored large-area ionospheric F2-layer was calm and electron density increased with decreasing latitude. PMID:27314360

  14. CGH analysis of secondary genetic changes in Ewing tumors: correlation with metastatic disease in a series of 43 cases.

    Science.gov (United States)

    Brisset, S; Schleiermacher, G; Peter, M; Mairal, A; Oberlin, O; Delattre, O; Aurias, A

    2001-10-01

    The occurrence of secondary chromosome changes is frequent in Ewing tumors, in particular trisomies for chromosomes 8 and 12, and unbalanced (1;16) translocations leading to gains of 1q and losses of 16q. The prognostic value of these secondary aberrations has not been statistically demonstrated. We report here a CGH analysis of a series of 43 primary tumors corresponding to 21 localized and 22 metastatic tumors. For five of them, a sufficient amount of DNA for the CGH analysis was available from the frozen samples. For 19 samples, a preliminary step of DOP-PCR amplification of the DNA was necessary. For the last 19 tumors, DNA was obtained after DOP-PCR amplification of small amount of DNA contaminating the RNA. As a whole, the main chromosome imbalances previously described, such as trisomies for 1q, 8, and 12, were observed. It is noteworthy that the mean number of imbalances was more frequent in localized versus metastatic tumors. Gain of 1q was more frequent in metastatic than in localized tumors. Nevertheless, these two results do not reach statistical significance. Conversely, a statistically significant excess of copy number of chromosome 2 was observed in non-metastatic tumors, suggesting that this imbalance, which has never been previously reported, could be associated with more favorable tumor behavior. PMID:11672775

  15. Design and implementation of a space domain spherical microphone array with application to source localization and separation.

    Science.gov (United States)

    Bai, Mingsian R; Yao, Yueh Hua; Lai, Chang-Sheng; Lo, Yi-Yang

    2016-03-01

    In this paper, four delay-and-sum (DAS) beamformers formulated in the modal domain and the space domain for open and solid spherical apertures are examined through numerical simulations. The resulting beampatterns reveal that the mainlobe of the solid spherical DAS array is only slightly narrower than that of the open array, whereas the sidelobes of the modal domain array are more significant than those of the space domain array due to the discrete approximation of continuous spherical Fourier transformation. To verify the theory experimentally, a three-dimensionally printed spherical array on which 32 micro-electro-mechanical system microphones are mounted is utilized for localization and separation of sound sources. To overcome the basis mismatch problem in signal separation, source localization is first carried out using minimum variance distortionless response beamformer. Next, Tikhonov regularization (TIKR) and compressive sensing (CS) are employed to extract the source signal amplitudes. Simulations and experiments are conducted to validate the proposed spherical array system. Objective perceptual evaluation of speech quality test and a subjective listening test are undertaken in performance evaluation. The experimental results demonstrate better separation quality achieved by the CS approach than by the TIKR approach at the cost of computational complexity.

  16. Design and implementation of a space domain spherical microphone array with application to source localization and separation.

    Science.gov (United States)

    Bai, Mingsian R; Yao, Yueh Hua; Lai, Chang-Sheng; Lo, Yi-Yang

    2016-03-01

    In this paper, four delay-and-sum (DAS) beamformers formulated in the modal domain and the space domain for open and solid spherical apertures are examined through numerical simulations. The resulting beampatterns reveal that the mainlobe of the solid spherical DAS array is only slightly narrower than that of the open array, whereas the sidelobes of the modal domain array are more significant than those of the space domain array due to the discrete approximation of continuous spherical Fourier transformation. To verify the theory experimentally, a three-dimensionally printed spherical array on which 32 micro-electro-mechanical system microphones are mounted is utilized for localization and separation of sound sources. To overcome the basis mismatch problem in signal separation, source localization is first carried out using minimum variance distortionless response beamformer. Next, Tikhonov regularization (TIKR) and compressive sensing (CS) are employed to extract the source signal amplitudes. Simulations and experiments are conducted to validate the proposed spherical array system. Objective perceptual evaluation of speech quality test and a subjective listening test are undertaken in performance evaluation. The experimental results demonstrate better separation quality achieved by the CS approach than by the TIKR approach at the cost of computational complexity. PMID:27036243

  17. Design and validation of a large-format transition edge sensor array magnetic shielding system for space application

    Science.gov (United States)

    Bergen, A.; van Weers, H. J.; Bruineman, C.; Dhallé, M. M. J.; Krooshoop, H. J. G.; ter Brake, H. J. M.; Ravensberg, K.; Jackson, B. D.; Wafelbakker, C. K.

    2016-10-01

    The paper describes the development and the experimental validation of a cryogenic magnetic shielding system for transition edge sensor based space detector arrays. The system consists of an outer mu-metal shield and an inner superconducting niobium shield. First, a basic comparison is made between thin-walled mu-metal and superconducting shields, giving an off-axis expression for the field inside a cup-shaped superconductor as a function of the transverse external field. Starting from these preliminary analytical considerations, the design of an adequate and realistic shielding configuration for future space flight applications (either X-IFU [D. Barret et al., e-print arXiv:1308.6784 [astro-ph.IM] (2013)] or SAFARI [B. Jackson et al., IEEE Trans. Terahertz Sci. Technol. 2, 12 (2012)]) is described in more detail. The numerical design and verification tools (static and dynamic finite element method (FEM) models) are discussed together with their required input, i.e., the magnetic-field dependent permeability data. Next, the actual manufacturing of the shields is described, including a method to create a superconducting joint between the two superconducting shield elements that avoid flux penetration through the seam. The final part of the paper presents the experimental verification of the model predictions and the validation of the shield's performance. The shields were cooled through the superconducting transition temperature of niobium in zero applied magnetic field (<10 nT) or in a DC field with magnitude ˜100 μT, applied either along the system's symmetry axis or perpendicular to it. After cool-down, DC trapped flux profiles were measured along the shield axis with a flux-gate magnetometer and the attenuation of externally applied AC fields (100 μT, 0.1 Hz, both axial and transverse) was verified along this axis with superconducting quantum interference device magnetometers. The system's measured on-axis shielding factor is greater than 106, well exceeding

  18. Concept, design and implementation of a cardiovascular gene-centric 50 k SNP array for large-scale genomic association studies.

    Directory of Open Access Journals (Sweden)

    Brendan J Keating

    Full Text Available A wealth of genetic associations for cardiovascular and metabolic phenotypes in humans has been accumulating over the last decade, in particular a large number of loci derived from recent genome wide association studies (GWAS. True complex disease-associated loci often exert modest effects, so their delineation currently requires integration of diverse phenotypic data from large studies to ensure robust meta-analyses. We have designed a gene-centric 50 K single nucleotide polymorphism (SNP array to assess potentially relevant loci across a range of cardiovascular, metabolic and inflammatory syndromes. The array utilizes a "cosmopolitan" tagging approach to capture the genetic diversity across approximately 2,000 loci in populations represented in the HapMap and SeattleSNPs projects. The array content is informed by GWAS of vascular and inflammatory disease, expression quantitative trait loci implicated in atherosclerosis, pathway based approaches and comprehensive literature searching. The custom flexibility of the array platform facilitated interrogation of loci at differing stringencies, according to a gene prioritization strategy that allows saturation of high priority loci with a greater density of markers than the existing GWAS tools, particularly in African HapMap samples. We also demonstrate that the IBC array can be used to complement GWAS, increasing coverage in high priority CVD-related loci across all major HapMap populations. DNA from over 200,000 extensively phenotyped individuals will be genotyped with this array with a significant portion of the generated data being released into the academic domain facilitating in silico replication attempts, analyses of rare variants and cross-cohort meta-analyses in diverse populations. These datasets will also facilitate more robust secondary analyses, such as explorations with alternative genetic models, epistasis and gene-environment interactions.

  19. Conceptual design and optimization of a plastic scintillator array for 2D tomography using a compact D–D fast neutron generator

    International Nuclear Information System (INIS)

    A conceptual design optimization of a fast neutron tomography system was performed. The system is based on a compact deuterium–deuterium fast neutron generator and an arc-shaped array of individual neutron detectors. The array functions as a position sensitive one-dimensional detector allowing tomographic reconstruction of a two-dimensional cross section of an object up to 10 cm across. Each individual detector is to be optically isolated and consists of a plastic scintillator and a Silicon Photomultiplier for measuring light produced by recoil protons. A deterministic geometry-based model and a series of Monte Carlo simulations were used to optimize the design geometry parameters affecting the reconstructed image resolution. From this, it is expected that with an array of 100 detectors a reconstructed image resolution of ∼1.5 mm can be obtained. Other simulations were performed in order to optimize the scintillator depth (length along the neutron path) such that the best ratio of direct to scattered neutron counts is achieved. This resulted in a depth of 6–8 cm and an expected detection efficiency of 33–37%. Based on current operational capabilities of a prototype neutron generator being developed at the Paul Scherrer Institute, planned implementation of this detector array design should allow reconstructed tomograms to be obtained with exposure times on the order of a few hours. - Highlights: • Conceptual design and optimization of a 2D fast neutron tomography system were performed. • Monte Carlo simulations were used to estimate ∼1.5 mm resolution and negligible scattering effects. • Geometry-based deterministic model was developed and used to verify the Monte Carlo results

  20. Comparison of Ambient Noise From Two Station Designs, Evaluating USArray's Transportable and Flexible Arrays in the Pacific Northwest

    Science.gov (United States)

    Pfeifer, M.; Alvarez, M.; Woodward, R.; Yang, Z.

    2009-12-01

    The USArray program within the National Science Foundation-funded Earthscope program is comprised of two portable broadband seismic projects; the Transportable Array (TA), and the Flexible Array (FA). The TA consists of 400 stations occupy locations within the United States on a nominal 70 km spacing for a period of approximately 24 months. As a network, these TA stations roll from west to east so that within 10 years the entire lower 48 states will have been occupied by the TA network. As a complementary component of USArray, the FA pool of instruments is comprised of 1200 active-source, 120 short-period and 326 broadband portable stations. These instruments are used by Principal Investigator-driven studies which focus on geologic targets within the TA footprint. Currently the TA network is transitioning from the Rocky Mountains into the Great Plains. The FA currently has four experiments installed. In this study we quantify the overall performance of these two tandem networks using a controlled set of continuous recordings in Western Washington. We compare the background noise levels between the standard deep TA and shallow FA broadband sensor vault system. We use McNamara’s probability density function (PDF) analysis as the basis of the comparison. We combine the network wide PDF’s of each network for a period of over 600 days of contemporaneous recordings. Preliminary analysis using data from 28 TA stations in western Washington and 47 nearby FA stations from the CAFE experiment (Abers, et al. Eos Trans. AGU 88(52), Fall Meet. Suppl. S43D-07), show that the TA stations are quieter at periods below 20 seconds by about 12 dB on the horizontal components. The vertical components for both the TA and FA are equivalent for periods below 5 seconds. At higher frequencies (> 2 Hz), however, the FA shallower vault is quieter by approximately 10 dB on both the vertical and horizontal components. The question addressed is, what is contributing to the difference in

  1. Copy Number Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplification.

    Science.gov (United States)

    Dimitriadou, Eftychia; Zamani Esteki, Masoud; Vermeesch, Joris Robert

    2015-01-01

    Whole genome amplification is required to ensure the availability of sufficient material for copy number variation analysis of a genome deriving from an individual cell. Here, we describe the protocols we use for copy number variation analysis of non-fixed single cells by array-based approaches following single-cell isolation and whole genome amplification. We are focusing on two alternative protocols, an isothermal and a PCR-based whole genome amplification method, followed by either comparative genome hybridization (aCGH) or SNP array analysis, respectively.

  2. Design of 8 Elements Wideband Circularly Polarized Microstrip Array Antenna%8元宽频带圆极化微带天线阵的设计

    Institute of Scientific and Technical Information of China (English)

    胡永金; 丁卫平; 柴仁文

    2011-01-01

    采用空气夹层的双层圆形微带贴片结构,通过Wilkinson功分移相器进行馈电,设计了宽带圆极化天线单元以及8元圆极化微带阵列天线,并加工制作了天线阵实物。实测结果表明:该阵列天线在2.0GHz2.5GHz频率范围内,圆极化轴比小于3dB,回波损耗小于一10dB,增益大于12dB,满足S频段卫星测控系统天线的指标要求。%Double-layer circular microstrip patch with air interlayer structure and Wilkinson power divider series phase shifter were applied to the design of wideband circularly polarized microstrip antenna element and 8 elements array antenna. The array antenna was fabricated and measured, with the axial ratio of the array antenna below 3 dB from 2.0 to 2. 5 GHz, its return loss below --10 dB, and its gain more than 12 dB in the bandwidth. Measured results show that the array antenna satisfies the requirements of S band satellite telemetry and control system application.

  3. Design of a stacked diode array pumped Nd:YAG laser with a truncated compound parabolic concentrator and a diffusive optical cavity

    CERN Document Server

    Lee, S M; Yun, M J; Cha, B H; Lee, J M

    1999-01-01

    We designed and analyzed a new Nd:YAG laser which employs a combination of a truncated compound parabolic concentrator (CPC) and a diffusive optical pump cavity for pumping with a stacked diode array. A design code based on a ray-tracing algorithm was developed to analyze the Nd:YAG laser system. The optimum truncated CPC profile was determined from analyses of the dependences of the pump-beam distribution and the total absorbed power in the laser crystal upon the acceptance angle and the slit width of the CPC. In addition, the analyses show the importance of the acceptance angle in designing an efficient laser system with a truncated CPC. Using conservative design parameters, the design code predicted a Nd:YAG laser output of 130 W with a corresponding optical slope efficiency of 44%.

  4. Design of Multi-unit Control System of High Voltage Power Supply for LASCAR Scintillator Detector Array

    Institute of Scientific and Technical Information of China (English)

    WuLijie; WangJinchuan; XiaoGuoqing; GuoZhongyan; ZhanWenlong; QiHuirong; XuZhiguo; ZhangLi; DingXianli; XuHushan; SunZhiyu; LiJiaxing; LiChen; WangMeng; ChenLixin; HuZhengguo; MaoRuishi; ZhaoTiecheng

    2003-01-01

    The power voltages of Photomultipliers (PMTs) at RIBLL LASCAR scintillator detector array are distributed between 900 V and 1 800 V irregularly. 392 CC123 modules are employed to supply high voltage for the PMT array. The CC123 module serves as PMT interface groupware package, and it can transform +12 V DC input voltage to ranges of 0~-2200 V for the PMT power supply corresponding to 0~+5 V output voltage from the control board crate. The relation of PMT power supply with the output voltage of the control crate is shown in Fig.1.

  5. A design for a linear array PIN photodiode for use in a Computed mammo-Tomography (CmT) system

    Energy Technology Data Exchange (ETDEWEB)

    Park, Shin-Woong [Department of Electronics and Information Engineering, Korea University, 1.5ka Anam-dong, Sungbuk-gu, Seoul 136-701 (Korea, Republic of); Yuk, Sunwoo [Department of Electronics and Control, Korea Orthopedics and Rehabilitation Engineering Center, 47-3, Gusan-dong, Bupyung-gu, Incheon 403-712 (Korea, Republic of); Park, Jung-Byung [DRGEM Corporation, 6th Floor Research Institute, Asan Medical Center, Pungnap-2dong, Songpa-gu, Seoul 138-736 (Korea, Republic of); Yi, Yun [Department of Electronics and Information Engineering, Korea University, 1.5ka Anam-dong, Sungbuk-gu, Seoul 136-701 (Korea, Republic of)], E-mail: yunyi@korea.ac.kr

    2009-10-21

    A p-i-n (PIN) photodiode has been used in a solid-state detector for X-ray detection as a photosensor of visible light from the scintillator. The most sensitive material used as low-energy X-ray detector in the mammography system is a Gd{sub 2}O{sub 2}S (GOS). As the light from GOS having a short wavelength in the range of 450-700 nm (peak at 510 nm) is absorbed within a very shallow layer near the surface of photodiode before arriving at depletion region and does not contribute to the signal. For designing the PIN photodiode, it is important to make p-layer as shallow as possible. In order to achieve shallow junction, the optimum conditions of ion implantation such as thickness of SiO{sub 2} oxide barrier, tilting angle of the wafer with respect to incident ion beam, and annealing conditions, have been determined using simulation results. The penetration depths are about 2 {mu}m for 510 nm, and 7 {mu}m for 700 nm. It is necessary for adequate depletion depth (about 10 {mu}m) to acquire the entire incident light. So far, wafers of {>=}1000 and {>=}150 {omega} cm resistivity were chosen, which offer about 15 and 6 {mu}m depletion depth, respectively. The pixel pitch of photodiode is 0.4 mmx3.0 mm and one module has 64 channels in linear array. Depth of the active p-layer is under 0.3 {mu}m in zero bias. Measured leakage currents under 10 pA/mm{sup 2} for both diodes and junction capacitances are 16 and 29 pF/mm{sup 2} in zero bias for the diodes of {>=}150 and {>=}1000 {omega} cm resistivity, respectively. The breast phantom, which was scanned by the Computed mammo-Tomography (CmT) system with two different detector modules and the data acquisition system, was developed. Little differences for distinct light absorption were shown in the three-dimensional images acquired in this study.

  6. The telescope control of the ASTRI SST-2M prototype for the Cherenkov telescope Array: hardware and software design architecture

    Science.gov (United States)

    Antolini, Elisa; Cascone, Enrico; Schwarz, Joseph; Stringhetti, Luca; Tanci, Claudio; Tosti, Gino; Aisa, Damiano; Aisa, Simone; Bagaglia, Marco; Busatta, Andrea; Campeggi, Carlo; Cefala, Marco; Farnesini, Lucio; Giacomel, Stefano; Marchiori, Gianpiero; Marcuzzi, Enrico; Nucciarelli, Giuliano; Piluso, Antonfranco

    2014-07-01

    ASTRI (Astrofisica con Specchi a Tecnologia Replicante Italiana) is a flagship project of the Italian Ministry of Research and led by the Italian National Institute of Astrophysics (INAF). One of its aims is to develop, within the Cherenkov Telescope Array (CTA) framework, an end-to-end small-sized telescope prototype in a dual-mirror configuration (SST-2M) in order to investigate the energy range E ~ 1-100 TeV. A long-term goal of the ASTRI program is the production of an ASTRI/CTA mini-array composed of seven SST-2M telescopes. The prototype, named ASTRI SST-2M, is seen as a standalone system that needs only network and power connections to work. The software system that is being developed to control the prototype is the base for the Mini-Array Software System (MASS), which has the task to make possible the operation of both the ASTRI SST-2M prototype and the ASTRI/CTA mini-array. The scope of this contribution is to give an overview of the hardware and software architecture adopted for the ASTRI SST- 2M prototype, showing how to apply state of the art industrial technologies to telescope control and monitoring systems.

  7. Design and microfabrication of a high-aspect-ratio PDMS microbeam array for parallel nanonewton force measurement and protein printing

    Science.gov (United States)

    Sasoglu, F. M.; Bohl, A. J.; Layton, B. E.

    2007-03-01

    Cell and protein mechanics has applications ranging from cellular development to tissue engineering. Techniques such as magnetic tweezers, optic tweezers and atomic force microscopy have been used to measure cell deformation forces of the order of piconewtons to nanonewtons. In this study, an array of polymeric polydimethylsiloxane (PDMS) microbeams with diameters of 10-40 µm and lengths of 118 µm was fabricated from Sylgard® with curing agent concentrations ranging from 5% to 20%. The resulting spring constants were 100-300 nN µm-1. The elastic modulus of PDMS was determined experimentally at different curing agent concentrations and found to be 346 kPa to 704 kPa in a millimeter-scale array and ~1 MPa in a microbeam array. Additionally, the microbeam array was used to print laminin for the purpose of cell adhesion. Linear and nonlinear finite element analyses are presented and compared to the closed-from solution. The highly compliant, transparent, biocompatible PDMS may offer a method for more rapid throughput in cell and protein mechanics force measurement experiments with sensitivities necessary for highly compliant structures such as axons.

  8. Design method of multidimensional dynamic arrays based on linked lists%基于链表结构的多维动态数组设计方法

    Institute of Scientific and Technical Information of China (English)

    闫沫; 王瑜

    2011-01-01

    To cope with problems such as the created dynamic arrays can not meet any dimension, any data type and also can not access data flexibly.A design method of multidimensional dynamic arrays is proposed.This method use linked lists to access data by pointer, and use variable-length argument lists technology to create any dimensional dynamic arrays.It is proved in practice that the method can be used in any data types and produce any dimensional arrays.One can both access an element from the created arrays with subscripts and access elements with linear order.The method can be extended easily and is more flexible than traditional methods.%针对动态数组生成中难以适应任意维数、任意数据类型、索引数据不够灵活等问题,提出一种基于链表结构的多维动态数组设计方法.该方法通过链表进行索引,采用变长参数列表技术动态生成数组.结果表明,该方法能够生成任意维动态数组;适用任意的数据类型;既能够直接利用数组下标索引数据,也能够对数据进行线性访问.与以往动态数组生成方法相比,更易于扩展.使用更加灵活.

  9. An Improved Optimization Design Method for Solar Photovoltaic Arrays and Engineering Application%光伏阵列改进优化设计方法与应用

    Institute of Scientific and Technical Information of China (English)

    丁明; 刘盛; 徐志成

    2013-01-01

    光伏阵列设计主要包括阵列间距、高度、倾角、光伏模块的选择以及安装方向的确定,设计方案的优劣直接关系到场地与光照的有效利用和投资效益。在场地尺寸给定条件下,这些因素相互牵制、共同影响光伏系统电能的输出,因此光伏阵列设计的优化十分必要。首先提出计算光伏阵列自身阴影损失的改进模型;然后在该模型的基础上,建立以最大发电量和最大收益为目标的阵列设计优化模型,运用粒子群算法求解,分析各参数对年发电量的影响;最后将该方法用于实际光伏屋顶电站系统设计,并获得满意效果,验证了该设计方法的合理性与工程实用价值。%Photovoltaic array design includes determination of array distance, height, inclined angle, PV module and installation direction. The performance of design scheme affects space and light usage and investment benefits directly. In a given field, these parameters affect the system energy output interacting with each other. Therefore, Photovoltaic array optimization is necessary. In the present paper, a loss model used to calculate PV array shading loss was improved. Then, the design problem of photovoltaic solar fields for PV system to obtain maximum annual output energy and maximum gains was formulated based on the loss model. Afterward, the Particle Swarm Optimization algorithm was applied for the solution of the PV design problem and the design variables impact analysis. Lastly, the method was applied to actual roof PV system design and obtained satisfied results. The results verify the rationality of the design method and its practical value in engineering.

  10. Design studies for a multi-TeV gamma-ray telescope array : PeX (PeV eXplorer)

    CERN Document Server

    Denman, Jarrad

    2014-01-01

    (Abridged) This thesis presents work towards the design of a new array of Image Atmospheric Cherenkov Telescopes (IACTs) to detect multi-TeV gamma-ray sources. The array consists of 5 telescopes in a square layout with one central telescope, known as the Pevatron eXplorer or PeX. PeX is a PeV (10^{15} eV) cosmic ray explorer that aims to study and discover gamma-ray sources in the 1 to 500 TeV range. The initial PeX design has been influenced by the HEGRA CT-System and H.E.S.S. configurations. One important feature of multi-TeV air showers is their ability to trigger telescopes at large core distance (>400m). PeX will utilise large core distance events to improve the performance and illustrate the viability of a sparse array for multi-TeV gamma-ray astronomy. One important aspect of the thesis (Chapter 6) was the investigation of a new time-based image cleaning method. The arrival time between photons in two adjacent pixels in the camera is used to apply an extra cut which helps mitigate night sky background....

  11. Design of a 7m Davies-Cotton Cherenkov telescope mount for the high energy section of the Cherenkov Telescope Array

    CERN Document Server

    Rovero, A C; Vallejo, G; Supanitsky, A D; Actis, M; Botani, A; Ochoa, I; Hughes, G

    2013-01-01

    The Cherenkov Telescope Array is the next generation ground-based observatory for the study of very-high-energy gamma-rays. It will provide an order of magnitude more sensitivity and greater angular resolution than present systems as well as an increased energy range (20 GeV to 300 TeV). For the high energy portion of this range, a relatively large area has to be covered by the array. For this, the construction of ~7 m diameter Cherenkov telescopes is an option under study. We have proposed an innovative design of a Davies-Cotton mount for such a telescope, within Cherenkov Telescope Array specifications, and evaluated its mechanical and optical performance. The mount is a reticulated-type structure with steel tubes and tensioned wires, designed in three main parts to be assembled on site. In this work we show the structural characteristics of the mount and the optical aberrations at the focal plane for three options of mirror facet size caused by mount deformations due to wind and gravity.

  12. “Distributed hybrid” MH–CGH2 system for hydrogen storage and its supply to LT PEMFC power modules

    Energy Technology Data Exchange (ETDEWEB)

    Lototskyy, M., E-mail: mlototskyy@uwc.ac.za [HySA Systems Competence Centre, South African Institute for Advanced Materials Chemistry, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville 7535 (South Africa); Tolj, I.; Davids, M.W.; Bujlo, P. [HySA Systems Competence Centre, South African Institute for Advanced Materials Chemistry, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville 7535 (South Africa); Smith, F. [Impala Platinum Ltd, Springs (South Africa); Pollet, B.G. [HySA Systems Competence Centre, South African Institute for Advanced Materials Chemistry, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville 7535 (South Africa)

    2015-10-05

    Highlights: • Prototype hydrogen storage and supply system for LTPEMFC applications was developed. • Combination of MH and CGH2 tanks with common gas manifold was used. • Thermal coupling of fuel cell stack and MH tank was applied. • The system uses AB2-type MH; H2 equilibrium pressure ∼10 bar at room temperature. • Shorter H2 charge time and stable H2 supply at a fluctuating load were observed. - Abstract: This paper describes the layout and presents the results of the testing of a novel prototype “distributed hybrid” hydrogen storage and supply system that has the potential to be used for Low Temperature Proton Exchange Membrane Fuel Cell (LT-PEMFC) applications. The system consists of individual Metal Hydride (MH) and Compressed Gas (CGH2) tanks with common gas manifold, and a thermal management system where heat exchanger of the liquid heated-cooled MH tank is integrated with the cooling system of the LT-PEMFC BoP. The MH tank is filled with a medium-stability AB{sub 2}-type MH material (H{sub 2} equilibrium pressure of about 10 bar at room temperature). This innovative solution allows for (i) an increase in hydrogen storage capacity of the whole gas storage system and the reduction of H{sub 2} charge pressure; (ii) shorter charging times in the refuelling mode and smoother peaks of H{sub 2} consumption during its supply to the fuel cell stack; (iii) the use of standard parts with simple layout and lower costs; and (iv) adding flexibility in the layout and placement of the components of the hydrogen storage and supply system.

  13. Timed arrays wideband and time varying antenna arrays

    CERN Document Server

    Haupt, Randy L

    2015-01-01

    Introduces timed arrays and design approaches to meet the new high performance standards The author concentrates on any aspect of an antenna array that must be viewed from a time perspective. The first chapters briefly introduce antenna arrays and explain the difference between phased and timed arrays. Since timed arrays are designed for realistic time-varying signals and scenarios, the book also reviews wideband signals, baseband and passband RF signals, polarization and signal bandwidth. Other topics covered include time domain, mutual coupling, wideband elements, and dispersion. The auth

  14. Massive MIMO Antenna Array Design for 5 G Communication System%一种用于5G的大规模MIMO天线阵设计

    Institute of Scientific and Technical Information of China (English)

    毛建军; 于大群; 焦永昌

    2016-01-01

    设计了一种用于第五代移动通信系统的大规模MIMO天线阵。该天线阵采用8×8矩形排布,天线单元采用缝隙耦合馈电的贴片形式,通过蚀刻在上层地板上的两个正交H型缝隙对辐射贴片分别耦合馈电来实现±45°双线极化特性。所设计的天线阵工作在3.4 GHz~3.6 GHz,具有剖面低、结构紧凑、便于与射频前端集成化设计的特点,能够很好地满足下一代移动通信系统对天线阵的设计要求。%A massive MIMO antenna array for the 5G communication system is designed.The antenna array is with 8×8 elements and the rectangular arrangement.The patch fed by coupled slots is chosen as the array element, which realizes ±45°dual-linear polarization by the excitation of two orthogonal H shape aperture slots etched on the upper ground.The designed antenna covering 3.4 GHz~3.6 GHz frequency band has advantages such as low profile,compact structure and easy integration with RF frontend, which can meet the demand of antenna array for the next generation communication system.

  15. Design, simulation and characterization of a MEMS inertia switch with flexible CNTs/Cu composite array layer between electrodes for prolonging contact time

    International Nuclear Information System (INIS)

    This paper reports an inertia switch with a flexible carbon nanotubes/copper (CNTs/Cu) composite array layer between movable and fixed electrodes, which achieves a longer contact time compared to the traditional design using rigid-to-rigid impact between electrodes. The CNTs/Cu layer is fabricated using the composite electroplating method, and the whole device is completed by multi-layer metal electroplating based on the micro-electro-mechanical systems (MEMS) process. The dynamic responses of the designed inertia switch and the contact impact between a single CNT and a fixed electrode/another CNT have both been simulated by the ANSYS finite-element-method (FEM). It is shown that the contact time of the designed inertia switch is about 100 µs under the applied 80 g half-sine-shaped acceleration in the sensing direction. Finally, the fabricated MEMS inertia switch with the flexible CNTs/Cu composite array layer between electrodes has been evaluated by a dropping hammer system. The test contact time is about112 µs, which has a good agreement with the simulation and is much longer than that of the traditional design. (paper)

  16. Design and physicochemical characterisation of novel dissolving polymeric microneedle arrays for transdermal delivery of high dose, low molecular weight drugs

    OpenAIRE

    McCrudden, Maelíosa T. C.; Alkilani, Ahlam Zaid; MCCRUDDEN, CIAN M.; McAlister, Emma; McCarthy, Helen O.; Woolfson, A. David; Donnelly, Ryan F.

    2014-01-01

    We describe formulation and evaluation of novel dissolving polymeric microneedle (MN) arrays for the facilitated delivery of low molecular weight, high dose drugs. Ibuprofen sodium was used as the model here and was successfully formulated at approximately 50% w/w in the dry state using the copolymer poly(methylvinylether/maleic acid). These MNs were robust and effectively penetrated skin in vitro, dissolving rapidly to deliver the incorporated drug. The delivery of 1.5mg ibuprofen sodium, th...

  17. The advantage of using SNP array in clinical testing for hematological malignancies--a comparative study of three genetic testing methods.

    Science.gov (United States)

    Xu, Xinjie; Johnson, Eric B; Leverton, Lisa; Arthur, Ashley; Watson, Quinn; Chang, Faye L; Raca, Gordana; Laffin, Jennifer J

    2013-01-01

    Cytogenetic methods, including G-banded chromosome analysis and fluorescence in situ hybridization (FISH) analysis, serve as a critical part of routine clinical testing for hematological malignancies and provide important diagnostic and prognostic information; however, the limitations of cytogenetic methods, including the requirement for actively dividing cells and lower resolution of G-banded chromosome analysis as well as the inability of both G-banded chromosome analysis and FISH to detect copy number neutral loss of heterozygosity (CN-LOH), can result in a failure to detect genomic abnormalities with diagnostic and prognostic significance. Here, we compared the abnormality detection rate of clinically requested testing (i.e., G-banded chromosome analysis and FISH) with high-resolution oligo (i.e., array comparative genomic hybridization (aCGH)) and single-nucleotide polymorphism (SNP)/oligo hybrid (i.e., SNP-CGH) arrays in a series of patients, in an effort to assess the ability of newer technologies to overcome these limitations. This series found the detection rate for SNP-CGH to be 62.5% for myelodysplastic syndrome (MDS) cases and 72.7% for chronic lymphocytic leukemia (CLL) cases, which are significantly higher than the detection rates of aCGH (31.3% for MDS and 54.5% for CLL) and G-banding and/or FISH (43.8% for MDS and 54.5% for CLL). This demonstrates the advantages of combining SNP-CGH with conventional cytogenetics to provide comprehensive clinical information by detecting clonality, large balanced rearrangements, copy number aberrations, and CN-LOH.

  18. Analysis of copy number variation in the bovine genome

    Science.gov (United States)

    We initiated a systematic study of the copy number variation (CNV) within the Bovine HapMap cattle population using array comparative genomic hybridization (array CGH). Oligonucleotide CGH arrays were designed and fabricated to provide a genome-wide coverage with an average interval of 6 kb using t...

  19. A high-resolution map of copy number variation in the cattle genome

    Science.gov (United States)

    We conducted a systematic study of the cattle copy number variation (CNV) using array comparative genomic hybridization (array CGH). Oligonucleotide CGH arrays were designed and fabricated to provide a genome-wide coverage with an average interval of 6 kb using the Bta3.1 genome assembly. Dual-lab...

  20. Wafer-scale pixelated scintillator and specially designed data acquisition system for fiber optic taper array-coupled digital x-ray detector

    International Nuclear Information System (INIS)

    A digital x-ray detector scheme based on a pixelated scintillator coupled with a fiber optic (FOT) array is suitable for many high-resolution x-ray imaging applications. However, certain challenges need to be addressed for fabrication of wafer-scale uniform pixelated x-ray scintillators. In addition, difficulties associated with implementation of the data acquisition system for acquiring output image data from the multiple image sensors used in the detector also need to be addressed. In this paper, a 2×2 FOT array-coupled digital x-ray detector scheme using a 5-in. pixelated scintillator is proposed. A novel fabrication setup along with the corresponding processes for fabricating the wafer-scale pixelated scintillator and implementation of a specially designed embedded data acquisition system based on a single embedded micro-processer (ARM) and four field-programmable gate array (FPGA) chips are discussed in detail. Preliminary experiments demonstrate that this pixelated scintillator-based digital x-ray detector scheme with an active imaging area of about 100 mm×100 mm shows considerable potential for use in high-resolution x-ray imaging

  1. Wafer-scale pixelated scintillator and specially designed data acquisition system for fiber optic taper array-coupled digital x-ray detector

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, Zhigang; Li, Ji; Lei, Yaohu; Wang, Ru; Ren, Jianping; Qiao, Jian; Niu, Hanben, E-mail: szuoptoelectronics@163.com

    2015-09-21

    A digital x-ray detector scheme based on a pixelated scintillator coupled with a fiber optic (FOT) array is suitable for many high-resolution x-ray imaging applications. However, certain challenges need to be addressed for fabrication of wafer-scale uniform pixelated x-ray scintillators. In addition, difficulties associated with implementation of the data acquisition system for acquiring output image data from the multiple image sensors used in the detector also need to be addressed. In this paper, a 2×2 FOT array-coupled digital x-ray detector scheme using a 5-in. pixelated scintillator is proposed. A novel fabrication setup along with the corresponding processes for fabricating the wafer-scale pixelated scintillator and implementation of a specially designed embedded data acquisition system based on a single embedded micro-processer (ARM) and four field-programmable gate array (FPGA) chips are discussed in detail. Preliminary experiments demonstrate that this pixelated scintillator-based digital x-ray detector scheme with an active imaging area of about 100 mm×100 mm shows considerable potential for use in high-resolution x-ray imaging.

  2. β-galactosidase Production by Aspergillus niger ATCC 9142 Using Inexpensive Substrates in Solid-State Fermentation: Optimization by Orthogonal Arrays Design

    Science.gov (United States)

    Kazemi, Samaneh; Khayati, Gholam; Faezi-Ghasemi, Mohammad

    2016-01-01

    Background: Enzymatic hydrolysis of lactose is one of the most important biotechnological processes in the food industry, which is accomplished by enzyme β-galactosidase (β-gal, β-D-galactoside galactohydrolase, EC 3.2.1.23), trivial called lactase. Orthogonal arrays design is an appropriate option for the optimization of biotechnological processes for the production of microbial enzymes. Methods: Design of experimental (DOE) methodology using Taguchi orthogonal array (OA) was employed to screen the most significant levels of parameters, including the solid substrates (wheat straw, rice straw, and peanut pod), the carbon/nitrogen (C/N) ratios, the incubation time, and the inducer. The level of β-gal production was measured by a photometric enzyme activity assay using the artificial substrate ortho-Nitrophenyl-β-D-galactopyranoside. Results: The results showed that C/N ratio (0.2% [w/v], incubation time (144 hour), and solid substrate (wheat straw) were the best conditions determined by the design of experiments using the Taguchi approach. Conclusion: Our finding showed that the use of rice straw and peanut pod, as solid-state substrates, led to 2.041-folds increase in the production of the enzyme, as compared to rice straw. In addition, the presence of an inducer did not have any significant impact on the enzyme production levels.

  3. 波导窄边斜缝行波阵列天线设计%Design of waveguide narrow-side inclined slot non-resonant array antenna

    Institute of Scientific and Technical Information of China (English)

    陈晓鹏; 陈文俊; 石磊

    2014-01-01

    Theoretical computation and software simulation are combined to design waveguide narrow-side inclined slot non-resonant array antenna quickly. The electromagnetic simulation software HFSS is used to simulate the experimentation for getting the slot conductance function. After the initial design,the inclination angles and depth of the slots are adjusted to make actinal surface amplitude of the antenna approach the design value according to the difference got by comparing the simulated field dis-tribution with the theoretical distribution. A non-resonant linear array antenna with 47 array elements was designed. Another slot array was placed symmetrically to restrain the cross polarization. The simulated max side lobe is -24.8 dB while Taylor compre-hensive side lobe is -30 dB. The HPBW is 2.2° and the gain is 24.6 dB.%为了快速设计波导窄边斜缝行波阵列天线,采用理论计算结合软件仿真的方法。利用电磁仿真软件HFSS模拟实验过程,得到裂缝电导函数。初始设计完成后,比较仿真结果口径分布和理论口径分布的差异,微调裂缝尺寸参数使天线口面幅度逼近设计值。设计了一个47阵元行波线阵,并采用两根波导对称放置抑制交叉极化。Taylor综合副瓣值为-30 dB,仿真得到的最大副瓣值为-24.8 dB,波瓣宽度为2.2°,增益为24.6 dB。

  4. A Model Analysis for the Design and Deployment of an Eulerian Sediment Trap Mooring Array in a Western Boundary Upwelling System from Southeast Brazil

    Directory of Open Access Journals (Sweden)

    URSULA MENDOZA

    2014-06-01

    Full Text Available This work addresses the design and configuration of a Eulerian sediment trap mooring array, which was deployed at the shelf edge (zm ≈ 140 m 80 km off Cabo Frio, SE- Brazil (23° S. The site was subject to interplay between the Tropical Waters (TW of the Brazil Current (BC, intrusions from the South Atlantic Central Waters (SACW, which are the source of upwelling in the region, and other oceanographic processes. Detailed computations were used to optimize the total weight, buoyancy balance, and maximum acceptable tilt to avoid hydrodynamic bias in the trapping efficiency and array adaptation to the local oceanographic conditions with the assistance of Matlab and Muringa programs and Modular Ocean Model 4.0 (MOM; i.e., to assert the vertical distribution of the meridional current component. The velocity range of the current component was determined by short term measurements to be between 0.1 and 0.5 m/s. Projections led to a resulting minimum anchor weight of 456 kg. The necessary line tension was ascertained by using the appropriate distribution of a series of buoys along the array, which finally attained a high vertical load of 350 kg because of the attached oceanographic equipment. Additional flotation devices resulted in a stable mooring array as reflected by their low calculated tilt (2.6° ± 0.6°. A low drag of 16 N was computed for the maximum surface current velocity of 0.5 m/s. The Reynolds number values ranged from 4 × 104 to 2 × 105 and a cone-trap aspect ratio of 1.75 was used to assess the trap sampling efficiency upon exposure to different current velocities.

  5. Contribution to the design of waveguide-fed compound-slot arrays by means of equivalent circuit modelling

    OpenAIRE

    Montesinos Ortego, Ignacio

    2012-01-01

    Los arrays de ranuras son sistemas de antennas conocidos desde los años 40, principalmente destinados a formar parte de sistemas rádar de navíos de combate y grandes estaciones terrenas donde el tamaño y el peso no eran altamente restrictivos. Con el paso de los años y debido sobre todo a importantes avances en materiales y métodos de fabricación, el rango de aplicaciones de este tipo de sistemas radiantes creció en gran medida. Desde nuevas tecnologías biomédicas, sistemas anticolisión en au...

  6. Next generation genome-wide association tool: Design and coverage of a high-throughput European-optimized SNP array

    Science.gov (United States)

    Hoffmann, Thomas J.; Kvale, Mark N.; Hesselson, Stephanie E.; Zhan, Yiping; Aquino, Christine; Cao, Yang; Cawley, Simon; Chung, Elaine; Connell, Sheryl; Eshragh, Jasmin; Ewing, Marcia; Gollub, Jeremy; Henderson, Mary; Hubbell, Earl; Iribarren, Carlos; Kaufman, Jay; Lao, Richard Z.; Lu, Yontao; Ludwig, Dana; Mathauda, Gurpreet K.; McGuire, William; Mei, Gangwu; Miles, Sunita; Purdy, Matthew M.; Quesenberry, Charles; Ranatunga, Dilrini; Rowell, Sarah; Sadler, Marianne; Shapero, Michael H.; Shen, Ling; Shenoy, Tanushree R.; Smethurst, David; Van den Eeden, Stephen K.; Walter, Larry; Wan, Eunice; Wearley, Reid; Webster, Teresa; Wen, Christopher C.; Weng, Li; Whitmer, Rachel A.; Williams, Alan; Wong, Simon C.; Zau, Chia; Finn, Andrea; Schaefer, Catherine; Kwok, Pui-Yan; Risch, Neil

    2011-01-01

    The success of genome-wide association studies has paralleled the development of efficient genotyping technologies. We describe the development of a next-generation microarray based on the new highly-efficient Affymetrix Axiom genotyping technology that we are using to genotype individuals of European ancestry from the Kaiser Permanente Research Program on Genes, Environment and Health (RPGEH). The array contains 674,517 SNPs, and provides excellent genome-wide as well as gene-based and candidate-SNP coverage. Coverage was calculated using an approach based on imputation and cross validation. Preliminary results for the first 80,301 saliva-derived DNA samples from the RPGEH demonstrate very high quality genotypes, with sample success rates above 94% and over 98% of successful samples having SNP call rates exceeding 98%. At steady state, we have produced 462 million genotypes per week for each Axiom system. The new array provides a valuable addition to the repertoire of tools for large scale genome-wide association studies. PMID:21565264

  7. 焦面场分析与相控阵馈源设计%Focal Field Analysis and Phased Array Feed Design

    Institute of Scientific and Technical Information of China (English)

    伍洋; 杜彪; 刘肖萌; 金乘进; 甘恒谦; 龚书喜

    2013-01-01

    A method of calculating focal field is introduced, and the connection between focal field, which depends on the reflector geometry, and the aperture field of feed is analyzed. Based on focal field distribution, a design method of phased array feed, including element spacing determination, phased array scale, and excitations for expected radiation pattern is presented. Two phased array feeds are designed to realize shaped beams for an un-shaped Cassegrain antenna and shaped scanning beams for a prime-focus parabolic antenna respectively. The numerical analyses demonstrate the effectiveness of the approach.%  该文介绍了焦面场的计算方法及其与反射面焦径比的关系,分析了焦面场与馈源口径场的联系,并建立了基于焦面场分析进行相控阵馈源设计的方法,包括确定单元间距、阵列规模以及实现目标方向图所需要的激励。按照这一方法,分别以未赋形卡塞格伦天线和前馈抛物面天线为对象,设计了各自的相控阵馈源以实现赋形波束的效果,并在后者上验证了波束扫描性能。通过仿真,天线性能实现了预定目标,证明了设计方法的有效性。

  8. 二维DOA估计中麦克风阵列优化设计%Microphone array optimization design for two-dimensional DOA estimation

    Institute of Scientific and Technical Information of China (English)

    王冬霞; 齐畅; 周城旭; 牛芳琳

    2015-01-01

    在DOA估计中,往往事先假设麦克风阵列的结构,然后通过改进DOA估计方法来提高定位精度,忽视了麦克风的摆放位置对 DOA 估计性能的影响。基于此,针对二维 DOA 估计,提出改进遗传优化算法,将空时滤波器系数和麦克风阵列结构分开,构造由二维 MUSIC空间谱函数欧式距离和优化后阵元个数共为变量的适应度函数,以 DOA 估计精度为停止条件,对均匀矩形阵、均匀圆形阵和均匀同心圆阵开展优化设计。仿真结果表明,采用所提方法优化后的阵列取得了较好的DOA估计性能。%The studies usually focus on how to give an effective method to improve position accuracy with the known-microphone array configurations for the DOA estimation.However,the position of the microphone influences the overall performance of DOA estimation. An improved genetic optimization algorithm is thus proposed for two-dimensional DOA estimation.Its fitness function consists of the Euclidean distance of the 2-D MUSIC spatial spectrum function and microphone numbers after optimization,which separates the space-time filter coefficients from microphone array configuration.And DOA estimation precision is adopted as stop condition of genetic algorithm. Uniform rectangular array,uniform circular array and uniform concentric circular array are used to carry out optimization design. The simulation results show that effective DOA estimation performances are obtained after optimization with the proposed method.

  9. Design concepts for the Cherenkov Telescope Array CTA: an advanced facility for ground-based high-energy gamma-ray astronomy

    International Nuclear Information System (INIS)

    Ground-based gamma-ray astronomy has had a major breakthrough with the impressive results obtained using systems of imaging atmospheric Cherenkov telescopes. Ground-based gamma-ray astronomy has a huge potential in astrophysics, particle physics and cosmology. CTA is an international initiative to build the next generation instrument, with a factor of 5-10 improvement in sensitivity in the 100 GeV-10 TeV range and the extension to energies well below 100 GeV and above 100 TeV. CTA will consist of two arrays (one in the north, one in the south) for full sky coverage and will be operated as open observatory. The design of CTA is based on currently available technology. This document reports on the status and presents the major design concepts of CTA. (authors)

  10. 3-D Imaging Using Row–Column-Addressed Arrays With Integrated Apodization. Part I: Apodization Design and Line Element Beamforming

    DEFF Research Database (Denmark)

    Rasmussen, Morten Fischer; Christiansen, Thomas Lehrmann; Thomsen, Erik Vilain;

    2015-01-01

    ghost echo intensity of a scatterer at (x,y, z) = (8, 3, 30) mm was decreased by 43 dB by integrating roll-off apodization into the array. The main lobe was unaffected by the apodization. Simulations of a 3-mm-diameter anechoic blood vessel at 30 mm depth showed that applying the transducer......-integrated apodization increased the apparent diameter of the vessel from 2.0 mm to 2.4 mm, corresponding to an increase from 67% to 80% of the true vessel diameter. The line element beamforming approach is shown to be essential for achieving correct time-of-flight calculations, and hence avoid geometrical distortions...

  11. Broadband impedance-matched near-zero-index metamaterials for a wide scanning phased array antenna design

    International Nuclear Information System (INIS)

    We present broadband near-zero-index metamaterials composed of dielectric resonators and metallic rods, whose permittivity ε and permeability μ are near-zero simultaneously. It is notable that the values of permittivity ε are equal to those of permeability μ over a broadband frequency range of 8.45 GHz to 10.5 GHz, indicating the impedances of the proposed near-zero-index metamaterials match vacuum in this broadband. The broadband near-zero-index metamaterials for manipulating radiation sources are analyzed. We also demonstrate numerically that such near-zero-index metamaterials can offer a unique grating condition in a phased array antenna, with the beam scanning angle range beyond the critical angle limit of the grating lobe. (paper)

  12. aCGH Analysis to Estimate Genetic Variations among Domesticated Chickens.

    Science.gov (United States)

    Komiyama, Tomoyoshi; Lin, Mengjie; Ogura, Atsushi

    2016-01-01

    Chickens have been familiar to humans since ancient times and have been used not only for culinary purposes but also for cultural purposes including ritual ceremonies and traditional entertainment. The various chicken breeds developed for these purposes often display distinct morphological and/or behavioural traits. For example, the Japanese Shamo is larger and more aggressive than other domesticated chickens, reflecting its role as a fighting cock breed, whereas Japanese Naganakidori breeds, which have long-crowing behaviour, were bred instead for their entertaining and aesthetic qualities. However, the genetic backgrounds of these distinct morphological and behavioural traits remain unclear. Therefore, the question arises as to which genomic regions in these chickens were acted upon by selective pressures through breeding. We compared the entire genomes of six chicken breeds domesticated for various cultural purposes by utilizing array comparative genomic hybridization. From these analyses, we identified 782 regions that underwent insertions, deletions, or mutations, representing man-made selection pressure in these chickens. Furthermore, we found that a number of genes diversified in domesticated chickens bred for cultural or entertainment purposes were different from those diversified in chickens bred for food, such as broilers and layers. PMID:27525263

  13. Design of a 5-MA 100-ns linear-transformer-driver accelerator for wire array Z-pinch experiments

    Science.gov (United States)

    Zhou, Lin; Li, Zhenghong; Wang, Zhen; Liang, Chuan; Li, Mingjia; Qi, Jianmin; Chu, Yanyun

    2016-03-01

    The linear-transformer-driver (LTD) is a recently developed pulsed-power technology that shows great promise for a number of applications. These include a Z -pinch-driven fission-fusion-hybrid reactor that is being developed by the Chinese Academy of Engineering Physics. In support of the reactor development effort, we are planning to build an LTD-based accelerator that is optimized for driving wire-array Z -pinch loads. The accelerator comprises six modules in parallel, each of which has eight series 0.8-MA LTD cavities in a voltage-adder configuration. Vacuum transmission lines are used from the interior of the adder to the central vacuum chamber where the load is placed. Thus the traditional stack-flashover problem is eliminated. The machine is 3.2 m tall and 12 m in outer diameter including supports. A prototype cavity was built and tested for more than 6000 shots intermittently at a repetition rate of 0.1 Hz. A novel trigger, in which only one input trigger pulse is needed by utilizing an internal trigger brick, was developed and successfully verified in these shots. A full circuit modeling was conducted for the accelerator. The simulation result shows that a current pulse rising to 5.2 MA in 91 ns (10%-90%) can be delivered to the wire-array load, which is 1.5 cm in height, 1.2 cm in initial radius, and 1 mg in mass. The maximum implosion velocity of the load is 32 cm /μ s when compressed to 0.1 of the initial radius. The maximum kinetic energy is 78 kJ, which is 11.7% of the electric energy stored in the capacitors. This accelerator is supposed to enable a radiation energy efficiency of 20%-30%, providing a high efficient facility for research on the fast Z pinch and technologies for repetition-rate-operated accelerators.

  14. 用于凹非球面的计算全息设计及其误差分析%Design and Error Analysis of the Computer-Generated Helogram Used for Concave Aspheric Surface Testing

    Institute of Scientific and Technical Information of China (English)

    冯婕; 邓超; 邢廷文

    2012-01-01

    In order to test concave aspheric surfaces precisely, a multifunctional computer-genera ted hologram (CGH) which can compensate wavefront in null test, adjust the relative position between CGH and interferometer and adjust the relative position between CGH and the tested aspheric surface is proposed and designed. It consists of three segments, called main CGH, alignment CGH and fiducial CGH. The principle of testing aspheric surfaces is reviewed and the design method is introduced. The pros and cons of the auxiliary CGH adjustment method and the common adjustment method are compared. Then a design example for testing a paraboloidal mirror (Φ187.72 mm、F number is 1.71) is presented. The effects of CGH fabrication errors on the accuracy of interferometric measurements are analyzed. The accuracy of using a CGH to measure the surface is 4. 47 nm. The designed CGH meets the requirements of high precision aspheric surfaces testing.%为实现非球面元件表面面形的高精度检测,提出并设计了一种兼具相位补偿、装调计算全息图(CGH)和待测非球面的多功能计算全息图.多功能计算全息图由主全息、对准全息和基准全息3部分组成.介绍了各部分的工作原理及其设计方法,比较了辅助全息装调方法和普通装调方法的优劣,并给出了检测Φ187.72 mm、F数为1.71的凹非球面镜的计算全息设计实例,分析了影响全息检测精度的各项误差源,计算得出所设计的计算全息的检测精度可达4.75 nm,实现了非球面元件面形的高精度检测.

  15. High-Resolution Analysis of Gene Copy Number Alterations in Human Prostate Cancer Using CGH on cDNA Microarrays: Impact of Copy Number on Gene Expression

    Directory of Open Access Journals (Sweden)

    Maija Wolf

    2004-05-01

    Full Text Available Identification of target genes for genetic rearrangements in prostate cancer and the impact of copy number changes on gene expression are currently not well understood. Here, we applied high-resolution comparative genomic hybridization (CGH on cDNA microarrays for analysis of prostate cancer cell lines. CGH microarrays identified most of the alterations detected by classical chromosomal CGH, as well as a number of previously unreported alterations. Specific recurrent regions of gain (28 and loss (18 were found, their boundaries defined with sub-megabasepair accuracy. The most common changes included copy number decreases at 13% and gains at iq and 5p. Refined mapping identified several sites, such as at 13q (33-44, 49-51, 74-76 Mbp from the p-telomere, which matched with minimal regions of loss seen in extensive loss of heterozygosity mapping studies of large numbers of tumors. Previously unreported recurrent changes were found at 2p, 2q, 3p, 17q (losses, at 3q, 5p, 6p (gains. Integration of genomic and transcriptomic data revealed the role of individual candidate target genes for genomic alterations as well as a highly significant (P < .0001 overall association between copy number levels and the percentage of differentially expressed genes. Across the genome, the overall impact of copy number on gene expression levels was, to a large extent, attributable to low-level gains and losses of copy number, corresponding to common deletions and gains of often large chromosomal regions.

  16. PAB-1, a Caenorhabditis elegans poly(A-binding protein, regulates mRNA metabolism in germline by interacting with CGH-1 and CAR-1.

    Directory of Open Access Journals (Sweden)

    Sunhee Ko

    Full Text Available Poly(A-binding proteins are highly conserved among eukaryotes and regulate stability of mRNA and translation. Among C. elegans homologues, pab-1 mutants showed defects in germline mitotic proliferation. Unlike pab-1 mutants, pab-1 RNAi at every larval stage caused arrest of germline development at the following stage, indicating that pab-1 is required for the entire postembryonic germline development. This idea is supported by the observations that the mRNA level of pab-1 increased throughout postembryonic development and its protein expression was germline-enriched. PAB-1 localized to P granules and the cytoplasm in the germline. PAB-1 colocalized with CGH-1 and CAR-1 and affected their localization, suggesting that PAB-1 is a component of processing (P-bodies that interacts with them. The mRNA and protein levels of representative germline genes, rec-8, GLP-1, rme-2, and msp-152, were decreased after pab-1 RNAi. Although the mRNA level of msp-152 was increased in cgh-1 mutant, it was also significantly reduced by pab-1 RNAi. Our results suggest that PAB-1 positively regulates the mRNA levels of germline genes, which is likely facilitated by the interaction of PAB-1 with other P-body components, CGH-1 and CAR-1.

  17. Design of Automatic Testing System for Active Phased Array Antenna%有源相控阵天线自动测试系统设计

    Institute of Scientific and Technical Information of China (English)

    熊毅; 符伟; 钱林; 何诚; 陶成忠

    2012-01-01

    Aimed at multi-channel, large amount of testing data, low test efficiency in T/R module test of active phased array antenna, we designed an automatic test system for active phased array antenna, which adapts to testing large amount of T/R modules. The system controls VNA and gathers data from it via GPIB or ethernet and fully uses strong ability of modern computer for processing and analyzing data. It not only solves the problems of trouble diagnosis and automatic calibration of active phased array, but also improves the work efficiency and saves human cost. Results of actual application show that the test system works stably, and is easy to operate, it can meet the demands for measuring T/R module.%针对现代有源相控阵雷达天线T/R组件测试通路多、测试数据处理量大、测试效率低的特点,设计了一种适用于大规模T/R组件测试的有源相控阵天线自动测试系统.该系统设计通过GPIB接口或以太网对VNA(矢量网络分析仪)进行访问控制与数据采集,充分利用了现代计算机强大的数据处理与分析能力,不仅解决了有源相控阵天线故障诊断与自动校正问题,而且还极大地提高了工作效率,节约了人力成本.实际应用表明:该测试系统工作稳定,使用方便,能够充分满足T/R组件测试需要.

  18. Design of Arrangement of Solar Array and Energy Storage in Solar Photovoltaic Generation%光伏阵列及储能的布置设计

    Institute of Scientific and Technical Information of China (English)

    韩学栋; 王海华

    2015-01-01

    Facing energy and environmental pressure, solar which is a kind natural renewable clean energy has become the focus of world development and utilization. This paper describes the optimal choice of the best dip angle of the solar array, deducing the layout spacing theoretical formula when the battery array in the horizontal plane and on the roof with a slope. Also, the paper describes the formula of the calculating components in series and parallel arrays of photovoltaic cells and storage cells in series and parallel. This paper provides some theoretical support to the design of the photovoltaic power generation system.%在能源和环境的双重压力下,太阳能这一清洁可再生的自然能源成为世界各国开发利用的重点。本文介绍了太阳能电池阵列最佳倾角的优化选择,推导了电池阵列在水平面以及带有一定坡度的屋面上进行布置时前后间距的理论计算公式,介绍了光伏电池阵列中组件的串并联以及储能蓄电池的串并联计算公式。上述工作对太阳能光伏发电系统的设计提供了一定的理论支撑。

  19. Design of a Weighted-Rotor Energy Harvester Based on Dynamic Analysis and Optimization of Circular Halbach Array Magnetic Disk

    OpenAIRE

    Yu-Jen Wang; Yu-Ti Hao; Hao-Yu Lin

    2015-01-01

    This paper proposes the design of a weighted-rotor energy harvester (WREH) in which the oscillation is caused by the periodic change of the tangential component of gravity, to harvest kinetic energy from a rotating wheel. When a WREH is designed with a suitable characteristic length, the rotor’s natural frequency changes according to the wheel rotation speed and the rotor oscillates at a wide angle and high angular velocity to generate a large amount of power. The magnetic disk is designed ac...

  20. Expandable LED array interconnect

    Science.gov (United States)

    Yuan, Thomas Cheng-Hsin; Keller, Bernd

    2011-03-01

    A light emitting device that can function as an array element in an expandable array of such devices. The light emitting device comprises a substrate that has a top surface and a plurality of edges. Input and output terminals are mounted to the top surface of the substrate. Both terminals comprise a plurality of contact pads disposed proximate to the edges of the substrate, allowing for easy access to both terminals from multiple edges of the substrate. A lighting element is mounted to the top surface of the substrate. The lighting element is connected between the input and output terminals. The contact pads provide multiple access points to the terminals which allow for greater flexibility in design when the devices are used as array elements in an expandable array.