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  1. Spatial normalization of array-CGH data

    Brennetot Caroline

    2006-05-01

    Full Text Available Abstract Background Array-based comparative genomic hybridization (array-CGH is a recently developed technique for analyzing changes in DNA copy number. As in all microarray analyses, normalization is required to correct for experimental artifacts while preserving the true biological signal. We investigated various sources of systematic variation in array-CGH data and identified two distinct types of spatial effect of no biological relevance as the predominant experimental artifacts: continuous spatial gradients and local spatial bias. Local spatial bias affects a large proportion of arrays, and has not previously been considered in array-CGH experiments. Results We show that existing normalization techniques do not correct these spatial effects properly. We therefore developed an automatic method for the spatial normalization of array-CGH data. This method makes it possible to delineate and to eliminate and/or correct areas affected by spatial bias. It is based on the combination of a spatial segmentation algorithm called NEM (Neighborhood Expectation Maximization and spatial trend estimation. We defined quality criteria for array-CGH data, demonstrating significant improvements in data quality with our method for three data sets coming from two different platforms (198, 175 and 26 BAC-arrays. Conclusion We have designed an automatic algorithm for the spatial normalization of BAC CGH-array data, preventing the misinterpretation of experimental artifacts as biologically relevant outliers in the genomic profile. This algorithm is implemented in the R package MANOR (Micro-Array NORmalization, which is described at http://bioinfo.curie.fr/projects/manor and available from the Bioconductor site http://www.bioconductor.org. It can also be tested on the CAPweb bioinformatics platform at http://bioinfo.curie.fr/CAPweb.

  2. Accurate confidence aware clustering of array CGH tumor profiles.

    van Houte, B.P.P.; Heringa, J.

    2010-01-01

    Motivation: Chromosomal aberrations tend to be characteristic for given (sub)types of cancer. Such aberrations can be detected with array comparative genomic hybridization (aCGH). Clustering aCGH tumor profiles aids in identifying chromosomal regions of interest and provides useful diagnostic

  3. Rapid prenatal diagnosis of cytogenetic abnormalities by array CGH analysis

    Array CGH analysis has been shown to be highly accurate for rapid detection of chromosomal aneuploidies and submicroscopic deletions or duplications on fetal DNA samples in a clinical prenatal diagnostic setting. The objective of this study is to present our "post-validation phase" experience with ...

  4. Two novel deletions (array CGH findings) in pigment dispersion syndrome.

    Mikelsaar, Ruth; Molder, Harras; Bartsch, Oliver; Punab, Margus

    2007-12-01

    We report the first male with pigment dispersion syndrome and a balanced translocation t(10;15)(p11.1;q11.1). Cytogenetic analyses using Giemsa banding and FISH methods, and array CGH were performed. Array CGH analyses did not show altered DNA sequences in the breakpoints of the translocation, but revealed two novel deletions in 2q22.1 and 18q22.1. We suppose that the coexistence of t(10;15) and pigment dispersion syndrome in our patient is a coincidence. The deletion in 2q22.1, where the gene LRP1B has been located, may play a major role in the dysembryogenesis of the eye and cause the disorder.

  5. CGHPRO – A comprehensive data analysis tool for array CGH

    Lenzner Steffen

    2005-04-01

    Full Text Available Abstract Background Array CGH (Comparative Genomic Hybridisation is a molecular cytogenetic technique for the genome wide detection of chromosomal imbalances. It is based on the co-hybridisation of differentially labelled test and reference DNA onto arrays of genomic BAC clones, cDNAs or oligonucleotides, and after correction for various intervening variables, loss or gain in the test DNA can be indicated from spots showing aberrant signal intensity ratios. Now that this technique is no longer confined to highly specialized laboratories and is entering the realm of clinical application, there is a need for a user-friendly software package that facilitates estimates of DNA dosage from raw signal intensities obtained by array CGH experiments, and which does not depend on a sophisticated computational environment. Results We have developed a user-friendly and versatile tool for the normalization, visualization, breakpoint detection and comparative analysis of array-CGH data. CGHPRO is a stand-alone JAVA application that guides the user through the whole process of data analysis. The import option for image analysis data covers several data formats, but users can also customize their own data formats. Several graphical representation tools assist in the selection of the appropriate normalization method. Intensity ratios of each clone can be plotted in a size-dependent manner along the chromosome ideograms. The interactive graphical interface offers the chance to explore the characteristics of each clone, such as the involvement of the clones sequence in segmental duplications. Circular Binary Segmentation and unsupervised Hidden Markov Model algorithms facilitate objective detection of chromosomal breakpoints. The storage of all essential data in a back-end database allows the simultaneously comparative analysis of different cases. The various display options facilitate also the definition of shortest regions of overlap and simplify the

  6. CGHnormaliter: an iterative strategy to enhance normalization of array CGH data with imbalanced aberrations.

    van Houte, B.P.P.; Binsl, T.W.; Hettling, H.; Pirovano, W.A.; Heringa, J.

    2009-01-01

    Background: Array comparative genomic hybridization (aCGH) is a popular technique for detection of genomic copy number imbalances. These play a critical role in the onset of various types of cancer. In the analysis of aCGH data, normalization is deemed a critical pre-processing step. In general,

  7. High-resolution SNP array analysis of patients with developmental disorder and normal array CGH results

    Siggberg Linda

    2012-09-01

    Full Text Available Abstract Background Diagnostic analysis of patients with developmental disorders has improved over recent years largely due to the use of microarray technology. Array methods that facilitate copy number analysis have enabled the diagnosis of up to 20% more patients with previously normal karyotyping results. A substantial number of patients remain undiagnosed, however. Methods and Results Using the Genome-Wide Human SNP array 6.0, we analyzed 35 patients with a developmental disorder of unknown cause and normal array comparative genomic hybridization (array CGH results, in order to characterize previously undefined genomic aberrations. We detected no seemingly pathogenic copy number aberrations. Most of the vast amount of data produced by the array was polymorphic and non-informative. Filtering of this data, based on copy number variant (CNV population frequencies as well as phenotypically relevant genes, enabled pinpointing regions of allelic homozygosity that included candidate genes correlating to the phenotypic features in four patients, but results could not be confirmed. Conclusions In this study, the use of an ultra high-resolution SNP array did not contribute to further diagnose patients with developmental disorders of unknown cause. The statistical power of these results is limited by the small size of the patient cohort, and interpretation of these negative results can only be applied to the patients studied here. We present the results of our study and the recurrence of clustered allelic homozygosity present in this material, as detected by the SNP 6.0 array.

  8. Phenotype in patients with intellectual disability and pathological results in array CGH.

    Caballero Pérez, V; López Pisón, F J; Miramar Gallart, M D; González Álvarez, A; García Jiménez, M C; García Iñiguez, J P; Orden Rueda, C; Gil Hernández, I; Fuertes Rodrigo, C; Fernando Martínez, R; Rodríguez Valle, A; Alcaine Villarroya, M J

    Global developmental delay (GDD) and intellectual disability (ID) are frequent reasons for consultation in paediatric neurology departments. Nowadays, array comparative genomic hybridisation (array-CGH) is one of the most widely used techniques for diagnosing these disorders. Our purpose was to determine the phenotypic features associated with pathological results in this genetic test. We conducted a blind study of the epidemiological, clinical, anthropometric, and morphological features of 80 patients with unexplained ID to determine which features were associated with pathological results in array-CGH. Pathological results were found in 27.5% of the patients. Factors associated with pathological results in array-CGH were a family history of GDD/ID (OR = 12.1), congenital malformations (OR = 5.33), having more than 3 facial dysmorphic features (OR = 20.9), and hypotonia (OR = 3.25). Our findings are consistent with those reported by other published series. We therefore conclude that the probability of having pathological results in array-CGH increases with the presence of any of the features mentioned above in patients with ID/GDD. Copyright © 2016 Sociedad Española de Neurología. Publicado por Elsevier España, S.L.U. All rights reserved.

  9. CGHnormaliter: a bioconductor package for normalization of array CGH data with many CNAs.

    van Houte, B.P.P.; Binsl, T.W.; Hettling, H.; Heringa, J.

    2010-01-01

    Summary: CGHnormaliter is a package for normalization of array comparative genomic hybridization (aCGH) data. It uses an iterative procedure that effectively eliminates the influence of imbalanced copy numbers. This leads to a more reliable assessment of copy number alterations (CNAs). CGHnormaliter

  10. Array CGH analysis of a cohort of Russian patients with intellectual disability.

    Kashevarova, Anna A; Nazarenko, Lyudmila P; Skryabin, Nikolay A; Salyukova, Olga A; Chechetkina, Nataliya N; Tolmacheva, Ekaterina N; Sazhenova, Elena A; Magini, Pamela; Graziano, Claudio; Romeo, Giovanni; Kučinskas, Vaidutis; Lebedev, Igor N

    2014-02-15

    The use of array comparative genomic hybridization (array CGH) as a diagnostic tool in molecular genetics has facilitated the identification of many new microdeletion/microduplication syndromes (MMSs). Furthermore, this method has allowed for the identification of copy number variations (CNVs) whose pathogenic role has yet to be uncovered. Here, we report on our application of array CGH for the identification of pathogenic CNVs in 79 Russian children with intellectual disability (ID). Twenty-six pathogenic or likely pathogenic changes in copy number were detected in 22 patients (28%): 8 CNVs corresponded to known MMSs, and 17 were not associated with previously described syndromes. In this report, we describe our findings and comment on genes potentially associated with ID that are located within the CNV regions. Copyright © 2013 Elsevier B.V. All rights reserved.

  11. Reliable single cell array CGH for clinical samples.

    Zbigniew T Czyż

    Full Text Available BACKGROUND: Disseminated cancer cells (DCCs and circulating tumor cells (CTCs are extremely rare, but comprise the precursors cells of distant metastases or therapy resistant cells. The detailed molecular analysis of these cells may help to identify key events of cancer cell dissemination, metastatic colony formation and systemic therapy escape. METHODOLOGY/PRINCIPAL FINDINGS: Using the Ampli1™ whole genome amplification (WGA technology and high-resolution oligonucleotide aCGH microarrays we optimized conditions for the analysis of structural copy number changes. The protocol presented here enables reliable detection of numerical genomic alterations as small as 0.1 Mb in a single cell. Analysis of single cells from well-characterized cell lines and single normal cells confirmed the stringent quantitative nature of the amplification and hybridization protocol. Importantly, fixation and staining procedures used to detect DCCs showed no significant impact on the outcome of the analysis, proving the clinical usability of our method. In a proof-of-principle study we tracked the chromosomal changes of single DCCs over a full course of high-dose chemotherapy treatment by isolating and analyzing DCCs of an individual breast cancer patient at four different time points. CONCLUSIONS/SIGNIFICANCE: The protocol enables detailed genome analysis of DCCs and thereby assessment of the clonal evolution during the natural course of the disease and under selection pressures. The results from an exemplary patient provide evidence that DCCs surviving selective therapeutic conditions may be recruited from a pool of genomically less advanced cells, which display a stable subset of specific genomic alterations.

  12. A new normalizing algorithm for BAC CGH arrays with quality control metrics.

    Miecznikowski, Jeffrey C; Gaile, Daniel P; Liu, Song; Shepherd, Lori; Nowak, Norma

    2011-01-01

    The main focus in pin-tip (or print-tip) microarray analysis is determining which probes, genes, or oligonucleotides are differentially expressed. Specifically in array comparative genomic hybridization (aCGH) experiments, researchers search for chromosomal imbalances in the genome. To model this data, scientists apply statistical methods to the structure of the experiment and assume that the data consist of the signal plus random noise. In this paper we propose "SmoothArray", a new method to preprocess comparative genomic hybridization (CGH) bacterial artificial chromosome (BAC) arrays and we show the effects on a cancer dataset. As part of our R software package "aCGHplus," this freely available algorithm removes the variation due to the intensity effects, pin/print-tip, the spatial location on the microarray chip, and the relative location from the well plate. removal of this variation improves the downstream analysis and subsequent inferences made on the data. Further, we present measures to evaluate the quality of the dataset according to the arrayer pins, 384-well plates, plate rows, and plate columns. We compare our method against competing methods using several metrics to measure the biological signal. With this novel normalization algorithm and quality control measures, the user can improve their inferences on datasets and pinpoint problems that may arise in their BAC aCGH technology.

  13. A New Normalizing Algorithm for BAC CGH Arrays with Quality Control Metrics

    Jeffrey C. Miecznikowski

    2011-01-01

    Full Text Available The main focus in pin-tip (or print-tip microarray analysis is determining which probes, genes, or oligonucleotides are differentially expressed. Specifically in array comparative genomic hybridization (aCGH experiments, researchers search for chromosomal imbalances in the genome. To model this data, scientists apply statistical methods to the structure of the experiment and assume that the data consist of the signal plus random noise. In this paper we propose “SmoothArray”, a new method to preprocess comparative genomic hybridization (CGH bacterial artificial chromosome (BAC arrays and we show the effects on a cancer dataset. As part of our R software package “aCGHplus,” this freely available algorithm removes the variation due to the intensity effects, pin/print-tip, the spatial location on the microarray chip, and the relative location from the well plate. removal of this variation improves the downstream analysis and subsequent inferences made on the data. Further, we present measures to evaluate the quality of the dataset according to the arrayer pins, 384-well plates, plate rows, and plate columns. We compare our method against competing methods using several metrics to measure the biological signal. With this novel normalization algorithm and quality control measures, the user can improve their inferences on datasets and pinpoint problems that may arise in their BAC aCGH technology.

  14. WebaCGH: an interactive online tool for the analysis and display of array comparative genomic hybridisation data.

    Frankenberger, Casey; Wu, Xiaolin; Harmon, Jerry; Church, Deanna; Gangi, Lisa M; Munroe, David J; Urzúa, Ulises

    2006-01-01

    Gene copy number variations occur both in normal cells and in numerous pathologies including cancer and developmental diseases. Array comparative genomic hybridisation (aCGH) is an emerging technology that allows detection of chromosomal gains and losses in a high-resolution format. When aCGH is performed on cDNA and oligonucleotide microarrays, the impact of DNA copy number on gene transcription profiles may be directly compared. We have created an online software tool, WebaCGH, that functions to (i) upload aCGH and gene transcription results from multiple experiments; (ii) identify significant aberrant regions using a local Z-score threshold in user-selected chromosomal segments subjected to smoothing with moving averages; and (iii) display results in a graphical format with full genome and individual chromosome views. In the individual chromosome display, data can be zoomed in/out in both dimensions (i.e. ratio and physical location) and plotted features can have 'mouse over' linking to outside databases to identify loci of interest. Uploaded data can be stored indefinitely for subsequent retrieval and analysis. WebaCGH was created as a Java-based web application using the open-source database MySQL. WebaCGH is freely accessible at http://129.43.22.27/WebaCGH/welcome.htm Xiaolin Wu (forestwu@mail.nih.gov) or Ulises Urzúa (uurzua@med.uchile.cl).

  15. Array-CGH Analysis in a Cohort of Phenotypically Well-Characterized Individuals with "Essential" Autism Spectrum Disorders

    Napoli, Eleonora; Russo, Serena; Casula, Laura; Alesi, Viola; Amendola, Filomena Alessandra; Angioni, Adriano; Novelli, Antonio; Valeri, Giovanni; Menghini, Deny; Vicari, Stefano

    2018-01-01

    Copy-number variants (CNVs) are associated with susceptibility to autism spectrum disorder (ASD). To detect the presence of CNVs, we conducted an array-comparative genomic hybridization (array-CGH) analysis in 133 children with "essential" ASD phenotype. Genetic analyses documented that 12 children had causative CNVs (C-CNVs), 29…

  16. A robust method to analyze copy number alterations of less than 100 kb in single cells using oligonucleotide array CGH.

    Birte Möhlendick

    Full Text Available Comprehensive genome wide analyses of single cells became increasingly important in cancer research, but remain to be a technically challenging task. Here, we provide a protocol for array comparative genomic hybridization (aCGH of single cells. The protocol is based on an established adapter-linker PCR (WGAM and allowed us to detect copy number alterations as small as 56 kb in single cells. In addition we report on factors influencing the success of single cell aCGH downstream of the amplification method, including the characteristics of the reference DNA, the labeling technique, the amount of input DNA, reamplification, the aCGH resolution, and data analysis. In comparison with two other commercially available non-linear single cell amplification methods, WGAM showed a very good performance in aCGH experiments. Finally, we demonstrate that cancer cells that were processed and identified by the CellSearch® System and that were subsequently isolated from the CellSearch® cartridge as single cells by fluorescence activated cell sorting (FACS could be successfully analyzed using our WGAM-aCGH protocol. We believe that even in the era of next-generation sequencing, our single cell aCGH protocol will be a useful and (cost- effective approach to study copy number alterations in single cells at resolution comparable to those reported currently for single cell digital karyotyping based on next generation sequencing data.

  17. De novo complex intra chromosomal rearrangement after ICSI: characterisation by BACs micro array-CGH

    Quimsiyeh Mazin

    2008-12-01

    Full Text Available Abstract Background In routine Assisted Reproductive Technology (ART men with severe oligozoospermia or azoospermia should be informed about the risk of de novo congenital or chromosomal abnormalities in ICSI program. Also the benefits of preimplantation or prenatal genetic diagnosis practice need to be explained to the couple. Methods From a routine ICSI attempt, using ejaculated sperm from male with severe oligozoospermia and having normal karyotype, a 30 years old pregnant woman was referred to prenatal diagnosis in the 17th week for bichorionic biamniotic twin gestation. Amniocentesis was performed because of the detection of an increased foetal nuchal translucency for one of the fetus by the sonographic examination during the 12th week of gestation (WG. Chromosome and DNA studies of the fetus were realized on cultured amniocytes Results Conventional, molecular cytogenetic and microarray CGH experiments allowed us to conclude that the fetus had a de novo pericentromeric inversion associated with a duplication of the 9p22.1-p24 chromosomal region, 46,XY,invdup(9(p22.1p24 [arrCGH 9p22.1p24 (RP11-130C19 → RP11-87O1x3]. As containing the critical 9p22 region, our case is in coincidence with the general phenotype features of the partial trisomy 9p syndrome with major growth retardation, microcephaly and microretrognathia. Conclusion This de novo complex chromosome rearrangement illustrates the possible risk of chromosome or gene defects in ICSI program and the contribution of array-CGH for mapping rapidly de novo chromosomal imbalance.

  18. High-resolution array CGH clarifies events occurring on 8p in carcinogenesis

    Cooke, Susanna L; Pole, Jessica CM; Chin, Suet-Feung; Ellis, Ian O; Caldas, Carlos; Edwards, Paul AW

    2008-01-01

    Rearrangement of the short arm of chromosome 8 (8p) is very common in epithelial cancers such as breast cancer. Usually there is an unbalanced translocation breakpoint in 8p12 (29.7 Mb – 38.5 Mb) with loss of distal 8p, sometimes with proximal amplification of 8p11-12. Rearrangements in 8p11-12 have been investigated using high-resolution array CGH, but the first 30 Mb of 8p are less well characterised, although this region contains several proposed tumour suppressor genes. We analysed the whole of 8p by array CGH at tiling-path BAC resolution in 32 breast and six pancreatic cancer cell lines. Regions of recurrent rearrangement distal to 8p12 were further characterised, using regional fosmid arrays. FISH, and quantitative RT-PCR on over 60 breast tumours validated the existence of similar events in primary material. We confirmed that 8p is usually lost up to at least 30 Mb, but a few lines showed focal loss or copy number steps within this region. Three regions showed rearrangements common to at least two cases: two regions of recurrent loss and one region of amplification. Loss within 8p23.3 (0 Mb – 2.2 Mb) was found in six cell lines. Of the genes always affected, ARHGEF10 showed a point mutation of the remaining normal copies in the DU4475 cell line. Deletions within 12.7 Mb – 19.1 Mb in 8p22, in two cases, affected TUSC3. A novel amplicon was found within 8p21.3 (19.1 Mb – 23.4 Mb) in two lines and one of 98 tumours. The pattern of rearrangements seen on 8p may be a consequence of the high density of potential targets on this chromosome arm, and ARHGEF10 may be a new candidate tumour suppressor gene

  19. The development of a mini-array for estimating the disease state of gastric adenocarcinoma by array CGH

    Oga Atsunori

    2008-12-01

    Full Text Available Abstract Background The treatment strategy usually depends on the disease state in the individual patient. However, it is difficult to estimate the disease state before treatment in many patients. The purpose of this study was to develop a BAC (bacterial artificial chromosome mini-array allowing for the estimation of node metastasis, liver metastasis, peritoneal dissemination and the depth of tumor invasion in gastric cancers. Methods Initially, the DNA copy number aberrations (DCNAs were analyzed by array-based comparative genomic hybridization (aCGH in 83 gastric adenocarcinomas as a training-sample set. Next, two independent analytical methods were applied to the aCGH data to identify the BAC clones with DNA copy number aberrations that were linked with the disease states. One of the methods, a decision-tree model classifier, identified 6, 4, 4, 4, and 7 clones for estimating lymph node metastasis, liver metastasis, peritoneal dissemination, depth of tumor invasion, and histological type, respectively. In the other method, a clone-by-clone comparison of the frequency of the DNA copy number aberrations selected 26 clones to estimate the disease states. Results By spotting these 50 clones together with 26 frequently or rarely involved clones and 62 reference clones, a mini-array was made to estimate the above parameters, and the diagnostic performance of the mini-array was evaluated for an independent set of 30 gastric cancers (blinded – sample set. In comparison to the clinicopathological features, the overall accuracy was 66.7% for node metastasis, 86.7% for liver metastasis, 86.7% for peritoneal dissemination, and 96.7% for depth of tumor invasion. The intratumoral heterogeneity barely affected the diagnostic performance of the mini-array. Conclusion These results suggest that the mini-array makes it possible to determine an optimal treatment for each of the patients with gastric adenocarcinoma.

  20. The development of a mini-array for estimating the disease state of gastric adenocarcinoma by array CGH

    Furuya, Tomoko; Uchiyama, Tetsuji; Adachi, Atsushi; Okada, Takae; Nakao, Motonao; Oga, Atsunori; Yang, Song-Ju; Kawauchi, Shigeto; Sasaki, Kohsuke

    2008-01-01

    The treatment strategy usually depends on the disease state in the individual patient. However, it is difficult to estimate the disease state before treatment in many patients. The purpose of this study was to develop a BAC (bacterial artificial chromosome) mini-array allowing for the estimation of node metastasis, liver metastasis, peritoneal dissemination and the depth of tumor invasion in gastric cancers. Initially, the DNA copy number aberrations (DCNAs) were analyzed by array-based comparative genomic hybridization (aCGH) in 83 gastric adenocarcinomas as a training-sample set. Next, two independent analytical methods were applied to the aCGH data to identify the BAC clones with DNA copy number aberrations that were linked with the disease states. One of the methods, a decision-tree model classifier, identified 6, 4, 4, 4, and 7 clones for estimating lymph node metastasis, liver metastasis, peritoneal dissemination, depth of tumor invasion, and histological type, respectively. In the other method, a clone-by-clone comparison of the frequency of the DNA copy number aberrations selected 26 clones to estimate the disease states. By spotting these 50 clones together with 26 frequently or rarely involved clones and 62 reference clones, a mini-array was made to estimate the above parameters, and the diagnostic performance of the mini-array was evaluated for an independent set of 30 gastric cancers (blinded – sample set). In comparison to the clinicopathological features, the overall accuracy was 66.7% for node metastasis, 86.7% for liver metastasis, 86.7% for peritoneal dissemination, and 96.7% for depth of tumor invasion. The intratumoral heterogeneity barely affected the diagnostic performance of the mini-array. These results suggest that the mini-array makes it possible to determine an optimal treatment for each of the patients with gastric adenocarcinoma

  1. CGHregions: Dimension Reduction for Array CGH Data with Minimal Information Loss

    Mark A. van de Wiel

    2007-01-01

    Full Text Available An algorithm to reduce multi-sample array CGH data from thousands of clones to tens or hundreds of clone regions is introduced. This reduction of the data is performed such that little information is lost, which is possible due to the high dependencies between neighboring clones. The algorithm is explained using a small example. The potential beneficial effects of the algorithm for downstream analysis are illustrated by re-analysis of previously published colorectal cancer data. Using multiple testing corrections suitable for these data, we provide statistical evidence for genomic differences on several clone regions between MSI+ and CIN+ tumors. The algorithm, named CGHregions, is available as an easy-to-use script in R.

  2. CGH and SNP array using DNA extracted from fixed cytogenetic preparations and long-term refrigerated bone marrow specimens

    MacKinnon Ruth N

    2012-02-01

    Full Text Available Abstract Background The analysis of nucleic acids is limited by the availability of archival specimens and the quality and amount of the extracted material. Archived cytogenetic preparations are stored in many laboratories and are a potential source of total genomic DNA for array karyotyping and other applications. Array CGH using DNA from fixed cytogenetic preparations has been described, but it is not known whether it can be used for SNP arrays. Diagnostic bone marrow specimens taken during the assessment of hematological malignancies are also a potential source of DNA, but it is generally assumed that DNA must be extracted, or the specimen frozen, within a day or two of collection, to obtain DNA suitable for further analysis. We have assessed DNA extracted from these materials for both SNP array and array CGH. Results We show that both SNP array and array CGH can be performed on genomic DNA extracted from cytogenetic specimens stored in Carnoy's fixative, and from bone marrow which has been stored unfrozen, at 4°C, for at least 36 days. We describe a procedure for extracting a usable concentration of total genomic DNA from cytogenetic suspensions of low cellularity. Conclusions The ability to use these archival specimens for DNA-based analysis increases the potential for retrospective genetic analysis of clinical specimens. Fixed cytogenetic preparations and long-term refrigerated bone marrow both provide DNA suitable for array karyotyping, and may be suitable for a wider range of analytical procedures.

  3. Array CGH Analysis and Developmental Delay: A Diagnostic Tool for Neurologists.

    Cameron, F; Xu, J; Jung, J; Prasad, C

    2013-11-01

    Developmental delay occurs in 1-3% of the population, with unknown etiology in approximately 50% of cases. Initial genetic work up for developmental delay previously included chromosome analysis and subtelomeric FISH (fluorescent in situ hybridization). Array Comparative Genomic Hybridization (aCGH) has emerged as a tool to detect genetic copy number changes and uniparental disomy and is the most sensitive test in providing etiological diagnosis in developmental delay. aCGH allows for the provision of prognosis and recurrence risks, improves access to resources, helps limit further investigations and may alter medical management in many cases. aCGH has led to the delineation of novel genetic syndromes associated with developmental delay. An illustrative case of a 31-year-old man with long standing global developmental delay and recently diagnosed 4q21 deletion syndrome with a deletion of 20.8 Mb genomic interval is provided. aCGH is now recommended as a first line test in children and adults with undiagnosed developmental delay and congenital anomalies. Puce d'hybridation génomique comparative et retard de développement : un outil diagnostic pour les neurologues. Le retard de développement survient chez 1 à 3% de la population et son étiologie est inconnue chez à peu près 50% des cas. L'évaluation génétique initiale pour un retard de développement incluait antérieurement une analyse chromosomique et une analyse par FISH (hybridation in situ en fluorescence) de régions subtélomériques. La puce d'hybridation génomique comparative (CGHa) est devenue un outil de détection des changements du nombre de copies géniques ainsi que de la disomie uniparentale et elle est le test le plus sensible pour fournir un diagnostic étiologique dans le retard de développement. Le CGHa permet d'offrir un pronostic et un risque de récurrence, améliore l'accès aux ressources, aide à limiter les évaluations et peut modifier le traitement médical dans bien des cas

  4. Comparative cytogenetic characterization of primary canine melanocytic lesions using array CGH and fluorescence in situ hybridization.

    Poorman, Kelsey; Borst, Luke; Moroff, Scott; Roy, Siddharth; Labelle, Philippe; Motsinger-Reif, Alison; Breen, Matthew

    2015-06-01

    Melanocytic lesions originating from the oral mucosa or cutaneous epithelium are common in the general dog population, with up to 100,000 diagnoses each year in the USA. Oral melanoma is the most frequent canine neoplasm of the oral cavity, exhibiting a highly aggressive course. Cutaneous melanocytomas occur frequently, but rarely develop into a malignant form. Despite the differential prognosis, it has been assumed that subtypes of melanocytic lesions represent the same disease. To address the relative paucity of information about their genomic status, molecular cytogenetic analysis was performed on the three recognized subtypes of canine melanocytic lesions. Using array comparative genomic hybridization (aCGH) analysis, highly aberrant distinct copy number status across the tumor genome for both of the malignant melanoma subtypes was revealed. The most frequent aberrations included gain of dog chromosome (CFA) 13 and 17 and loss of CFA 22. Melanocytomas possessed fewer genome wide aberrations, yet showed a recurrent gain of CFA 20q15.3-17. A distinctive copy number profile, evident only in oral melanomas, displayed a sigmoidal pattern of copy number loss followed immediately by a gain, around CFA 30q14. Moreover, when assessed by fluorescence in situ hybridization (FISH), copy number aberrations of targeted genes, such as gain of c-MYC (80 % of cases) and loss of CDKN2A (68 % of cases), were observed. This study suggests that in concordance with what is known for human melanomas, canine melanomas of the oral mucosa and cutaneous epithelium are discrete and initiated by different molecular pathways.

  5. Genome-Wide Screening of Cytogenetic Abnormalities in Multiple Myeloma Patients Using Array-CGH Technique: A Czech Multicenter Experience

    Jan Smetana

    2014-01-01

    Full Text Available Characteristic recurrent copy number aberrations (CNAs play a key role in multiple myeloma (MM pathogenesis and have important prognostic significance for MM patients. Array-based comparative genomic hybridization (aCGH provides a powerful tool for genome-wide classification of CNAs and thus should be implemented into MM routine diagnostics. We demonstrate the possibility of effective utilization of oligonucleotide-based aCGH in 91 MM patients. Chromosomal aberrations associated with effect on the prognosis of MM were initially evaluated by I-FISH and were found in 93.4% (85/91. Incidence of hyperdiploidy was 49.5% (45/91; del(13(q14 was detected in 57.1% (52/91; gain(1(q21 occurred in 58.2% (53/91; del(17(p13 was observed in 15.4% (14/91; and t(4;14(p16;q32 was found in 18.6% (16/86. Genome-wide screening using Agilent 44K aCGH microarrays revealed copy number alterations in 100% (91/91. Most common deletions were found at 13q (58.9%, 1p (39.6%, and 8p (31.1%, whereas gain of whole 1q was the most often duplicated region (50.6%. Furthermore, frequent homozygous deletions of genes playing important role in myeloma biology such as TRAF3, BIRC1/BIRC2, RB1, or CDKN2C were observed. Taken together, we demonstrated the utilization of aCGH technique in clinical diagnostics as powerful tool for identification of unbalanced genomic abnormalities with prognostic significance for MM patients.

  6. Combined use of expression and CGH arrays pinpoints novel candidate genes in Ewing sarcoma family of tumors

    Savola, Suvi; Knuutila, Sakari; Klami, Arto; Tripathi, Abhishek; Niini, Tarja; Serra, Massimo; Picci, Piero; Kaski, Samuel; Zambelli, Diana; Scotlandi, Katia

    2009-01-01

    Ewing sarcoma family of tumors (ESFT), characterized by t(11;22)(q24;q12), is one of the most common tumors of bone in children and young adults. In addition to EWS/FLI1 gene fusion, copy number changes are known to be significant for the underlying neoplastic development of ESFT and for patient outcome. Our genome-wide high-resolution analysis aspired to pinpoint genomic regions of highest interest and possible target genes in these areas. Array comparative genomic hybridization (CGH) and expression arrays were used to screen for copy number alterations and expression changes in ESFT patient samples. A total of 31 ESFT samples were analyzed by aCGH and in 16 patients DNA and RNA level data, created by expression arrays, was integrated. Time of the follow-up of these patients was 5–192 months. Clinical outcome was statistically evaluated by Kaplan-Meier/Logrank methods and RT-PCR was applied on 42 patient samples to study the gene of the highest interest. Copy number changes were detected in 87% of the cases. The most recurrent copy number changes were gains at 1q, 2, 8, and 12, and losses at 9p and 16q. Cumulative event free survival (ESFT) and overall survival (OS) were significantly better (P < 0.05) for primary tumors with three or less copy number changes than for tumors with higher number of copy number aberrations. In three samples copy number imbalances were detected in chromosomes 11 and 22 affecting the FLI1 and EWSR1 loci, suggesting that an unbalanced t(11;22) and subsequent duplication of the derivative chromosome harboring fusion gene is a common event in ESFT. Further, amplifications on chromosomes 20 and 22 seen in one patient sample suggest a novel translocation type between EWSR1 and an unidentified fusion partner at 20q. In total 20 novel ESFT associated putative oncogenes and tumor suppressor genes were found in the integration analysis of array CGH and expression data. Quantitative RT-PCR to study the expression levels of the most interesting

  7. Genotype-phenotype analysis of recombinant chromosome 4 syndrome: an array-CGH study and literature review.

    Hemmat, Morteza; Hemmat, Omid; Anguiano, Arturo; Boyar, Fatih Z; El Naggar, Mohammed; Wang, Jia-Chi; Wang, Borris T; Sahoo, Trilochan; Owen, Renius; Haddadin, Mary

    2013-05-02

    Recombinant chromosome 4, a rare constitutional rearrangement arising from pericentric inversion, comprises a duplicated segment of 4p13~p15→4pter and a deleted segment of 4q35→4qter. To date, 10 cases of recombinant chromosome 4 have been reported. We describe the second case in which array-CGH was used to characterize recombinant chromosome 4 syndrome. The patient was a one-year old boy with consistent clinical features. Conventional cytogenetics and FISH documented a recombinant chromosome 4, derived from a paternal pericentric inversion, leading to partial trisomy 4p and partial monosomy of 4q. Array-CGH, performed to further characterize the rearranged chromosome 4 and delineate the breakpoints, documented a small (4.36 Mb) 4q35.1 terminal deletion and a large (23.81 Mb) 4p15.1 terminal duplication. Genotype-phenotype analysis of 10 previously reported cases and the present case indicated relatively consistent clinical features and breakpoints. This consistency was more evident in our case and another characterized by array-CGH, where both showed the common breakpoints of p15.1 and q35.1. A genotype-phenotype correlation study between rec(4), dup(4p), and del(4q) syndromes revealed that urogenital and cardiac defects are probably due to the deletion of 4q whereas the other clinical features are likely due to 4p duplication. Our findings support that the clinical features of patients with rec(4) are relatively consistent and specific to the regions of duplication or deletion. Recombinant chromosome 4 syndrome thus appears to be a discrete entity that can be suspected on the basis of clinical features or specific deleted and duplicated chromosomal regions.

  8. Characterization of genomic alterations in radiation-associated breast cancer among childhood cancer survivors, using comparative genomic hybridization (CGH arrays.

    Xiaohong R Yang

    Full Text Available Ionizing radiation is an established risk factor for breast cancer. Epidemiologic studies of radiation-exposed cohorts have been primarily descriptive; molecular events responsible for the development of radiation-associated breast cancer have not been elucidated. In this study, we used array comparative genomic hybridization (array-CGH to characterize genome-wide copy number changes in breast tumors collected in the Childhood Cancer Survivor Study (CCSS. Array-CGH data were obtained from 32 cases who developed a second primary breast cancer following chest irradiation at early ages for the treatment of their first cancers, mostly Hodgkin lymphoma. The majority of these cases developed breast cancer before age 45 (91%, n = 29, had invasive ductal tumors (81%, n = 26, estrogen receptor (ER-positive staining (68%, n = 19 out of 28, and high proliferation as indicated by high Ki-67 staining (77%, n = 17 out of 22. Genomic regions with low-copy number gains and losses and high-level amplifications were similar to what has been reported in sporadic breast tumors, however, the frequency of amplifications of the 17q12 region containing human epidermal growth factor receptor 2 (HER2 was much higher among CCSS cases (38%, n = 12. Our findings suggest that second primary breast cancers in CCSS were enriched for an "amplifier" genomic subgroup with highly proliferative breast tumors. Future investigation in a larger irradiated cohort will be needed to confirm our findings.

  9. BAC CGH-array identified specific small-scale genomic imbalances in diploid DMBA-induced rat mammary tumors

    Samuelson, Emma; Karlsson, Sara; Partheen, Karolina; Nilsson, Staffan; Szpirer, Claude; Behboudi, Afrouz

    2012-01-01

    Development of breast cancer is a multistage process influenced by hormonal and environmental factors as well as by genetic background. The search for genes underlying this malignancy has recently been highly productive, but the etiology behind this complex disease is still not understood. In studies using animal cancer models, heterogeneity of the genetic background and environmental factors is reduced and thus analysis and identification of genetic aberrations in tumors may become easier. To identify chromosomal regions potentially involved in the initiation and progression of mammary cancer, in the present work we subjected a subset of experimental mammary tumors to cytogenetic and molecular genetic analysis. Mammary tumors were induced with DMBA (7,12-dimethylbenz[a]anthrazene) in female rats from the susceptible SPRD-Cu3 strain and from crosses and backcrosses between this strain and the resistant WKY strain. We first produced a general overview of chromosomal aberrations in the tumors using conventional kartyotyping (G-banding) and Comparative Genome Hybridization (CGH) analyses. Particular chromosomal changes were then analyzed in more details using an in-house developed BAC (bacterial artificial chromosome) CGH-array platform. Tumors appeared to be diploid by conventional karyotyping, however several sub-microscopic chromosome gains or losses in the tumor material were identified by BAC CGH-array analysis. An oncogenetic tree analysis based on the BAC CGH-array data suggested gain of rat chromosome (RNO) band 12q11, loss of RNO5q32 or RNO6q21 as the earliest events in the development of these mammary tumors. Some of the identified changes appear to be more specific for DMBA-induced mammary tumors and some are similar to those previously reported in ACI rat model for estradiol-induced mammary tumors. The later group of changes is more interesting, since they may represent anomalies that involve genes with a critical role in mammary tumor development. Genetic

  10. A 91 kb microdeletion at Xq26.2 involving the GPC3 gene in a female fetus with Simpson-Golabi-Behmel syndrome detected by prenatal arrayCGH

    Becher, Naja; Gjørup, Vibike; Christensen, Rikke

    2012-01-01

    A 91 kb microdeletion at Xq26.2 involving the GPC3 gene in a female fetus with Simpson-Golabi-Behmel syndrome detected by prenatal arrayCGH......A 91 kb microdeletion at Xq26.2 involving the GPC3 gene in a female fetus with Simpson-Golabi-Behmel syndrome detected by prenatal arrayCGH...

  11. Different Array CGH profiles within hereditary breast cancer tumors associated to BRCA1 expression and overall survival

    Alvarez, Carolina; Aravena, Andrés; Tapia, Teresa; Rozenblum, Ester; Solís, Luisa; Corvalán, Alejandro; Camus, Mauricio; Alvarez, Manuel; Munroe, David; Maass, Alejandro; Carvallo, Pilar

    2016-01-01

    Array CGH analysis of breast tumors has contributed to the identification of different genomic profiles in these tumors. Loss of DNA repair by BRCA1 functional deficiency in breast cancer has been proposed as a relevant contribution to breast cancer progression for tumors with no germline mutation. Identifying the genomic alterations taking place in BRCA1 not expressing tumors will lead us to a better understanding of the cellular functions affected in this heterogeneous disease. Moreover, specific genomic alterations may contribute to the identification of potential therapeutic targets and offer a more personalized treatment to breast cancer patients. Forty seven tumors from hereditary breast cancer cases, previously analyzed for BRCA1 expression, and screened for germline BRCA1 and 2 mutations, were analyzed by Array based Comparative Genomic Hybridization (aCGH) using Agilent 4x44K arrays. Overall survival was established for tumors in different clusters using Log-rank (Mantel-Cox) Test. Gene lists obtained from aCGH analysis were analyzed for Gene Ontology enrichment using GOrilla and DAVID tools. Genomic profiling of the tumors showed specific alterations associated to BRCA1 or 2 mutation status, and BRCA1 expression in the tumors, affecting relevant cellular processes. Similar cellular functions were found affected in BRCA1 not expressing and BRCA1 or 2 mutated tumors. Hierarchical clustering classified hereditary breast tumors in four major, groups according to the type and amount of genomic alterations, showing one group with a significantly poor overall survival (p = 0.0221). Within this cluster, deletion of PLEKHO1, GDF11, DARC, DAG1 and CD63 may be associated to the worse outcome of the patients. These results support the fact that BRCA1 lack of expression in tumors should be used as a marker for BRCAness and to select these patients for synthetic lethality approaches such as treatment with PARP inhibitors. In addition, the identification of specific

  12. Genomic analysis of CD8+ NK/T cell line, ‘SRIK-NKL’, with array-based CGH (aCGH), SKY/FISH and molecular mapping

    Rossi, Michael; LaDuca, Jeff; Cowell, John; Srivastava, Bejai I.S.; Matsui, Sei-ichi

    2010-01-01

    We performed aCGH, SKY /FISH, molecular mapping and expression analyses on a permanent CD8+ NK/T cell line, ‘SRIK-NKL’ established from a lymphoma (ALL) patient, in attempt to define the fundamental genetic profile of its unique NK phenotypes. aCGH revealed hemizygous deletion of 6p containing genes responsible for hematopoietic functions. The SKY demonstrated that a constitutive reciprocal translocation, rcpt(5;14)(p13.2;q11) is a stable marker. Using somatic hybrids containing der(5) derived from SRIK-NKL, we found that the breakpoint in one homologue of no. 5 is located upstream of IL7R and also that the breakpoint in no. 14 is located within TRA@. The FISH analysis using BAC which contains TRA@ and its flanking region further revealed a ~231 kb deletion within 14q11 in the der(5) but not in the normal homologue of no. 14. The RT-PCR analysis detected mRNA for TRA@ transcripts which were extending across, but not including, the deleted region. IL7R was detected at least at mRNA levels. These findings were consistent with the immunological findings that TRA@ and IL7R are both expressed at mRNA levels and TRA@ at cytoplasmic protein levels in SRIK-NKL cells. In addition to rept(5;14), aCGH identified novel copy number abnormalities suggesting that the unique phenotype of the SRIK-NKL cell line is not solely due to the TRA@ rearrangement. These findings provide supportive evidence for the notion that SRIK-NKL cells may be useful for studying not only the function of NK cells but also genetic deregulations associated with leukemiogenesis. PMID:17640729

  13. Detecting single DNA copy number variations in complex genomes using one nanogram of starting DNA and BAC-array CGH.

    Guillaud-Bataille, Marine; Valent, Alexander; Soularue, Pascal; Perot, Christine; Inda, Maria Mar; Receveur, Aline; Smaïli, Sadek; Roest Crollius, Hugues; Bénard, Jean; Bernheim, Alain; Gidrol, Xavier; Danglot, Gisèle

    2004-07-29

    Comparative genomic hybridization to bacterial artificial chromosome (BAC)-arrays (array-CGH) is a highly efficient technique, allowing the simultaneous measurement of genomic DNA copy number at hundreds or thousands of loci, and the reliable detection of local one-copy-level variations. We report a genome-wide amplification method allowing the same measurement sensitivity, using 1 ng of starting genomic DNA, instead of the classical 1 microg usually necessary. Using a discrete series of DNA fragments, we defined the parameters adapted to the most faithful ligation-mediated PCR amplification and the limits of the technique. The optimized protocol allows a 3000-fold DNA amplification, retaining the quantitative characteristics of the initial genome. Validation of the amplification procedure, using DNA from 10 tumour cell lines hybridized to BAC-arrays of 1500 spots, showed almost perfectly superimposed ratios for the non-amplified and amplified DNAs. Correlation coefficients of 0.96 and 0.99 were observed for regions of low-copy-level variations and all regions, respectively (including in vivo amplified oncogenes). Finally, labelling DNA using two nucleotides bearing the same fluorophore led to a significant increase in reproducibility and to the correct detection of one-copy gain or loss in >90% of the analysed data, even for pseudotriploid tumour genomes.

  14. Selection of single blastocysts for fresh transfer via standard morphology assessment alone and with array CGH for good prognosis IVF patients: results from a randomized pilot study

    Yang Zhihong

    2012-05-01

    Full Text Available Abstract Background Single embryo transfer (SET remains underutilized as a strategy to reduce multiple gestation risk in IVF, and its overall lower pregnancy rate underscores the need for improved techniques to select one embryo for fresh transfer. This study explored use of comprehensive chromosomal screening by array CGH (aCGH to provide this advantage and improve pregnancy rate from SET. Methods First-time IVF patients with a good prognosis (age Results For patients in Group A (n = 55, 425 blastocysts were biopsied and analyzed via aCGH (7.7 blastocysts/patient. Aneuploidy was detected in 191/425 (44.9% of blastocysts in this group. For patients in Group B (n = 48, 389 blastocysts were microscopically examined (8.1 blastocysts/patient. Clinical pregnancy rate was significantly higher in the morphology + aCGH group compared to the morphology-only group (70.9 and 45.8%, respectively; p = 0.017; ongoing pregnancy rate for Groups A and B were 69.1 vs. 41.7%, respectively (p = 0.009. There were no twin pregnancies. Conclusion Although aCGH followed by frozen embryo transfer has been used to screen at risk embryos (e.g., known parental chromosomal translocation or history of recurrent pregnancy loss, this is the first description of aCGH fully integrated with a clinical IVF program to select single blastocysts for fresh SET in good prognosis patients. The observed aneuploidy rate (44.9% among biopsied blastocysts highlights the inherent imprecision of SET when conventional morphology is used alone. Embryos randomized to the aCGH group implanted with greater efficiency, resulted in clinical pregnancy more often, and yielded a lower miscarriage rate than those selected without aCGH. Additional studies are needed to verify our pilot data and confirm a role for on-site, rapid aCGH for IVF patients contemplating fresh SET.

  15. Genome-wide detection of copy number variations among diverse horse breeds by array CGH.

    Wei Wang

    Full Text Available Recent studies have found that copy number variations (CNVs are widespread in human and animal genomes. CNVs are a significant source of genetic variation, and have been shown to be associated with phenotypic diversity. However, the effect of CNVs on genetic variation in horses is not well understood. In the present study, CNVs in 6 different breeds of mare horses, Mongolia horse, Abaga horse, Hequ horse and Kazakh horse (all plateau breeds and Debao pony and Thoroughbred, were determined using aCGH. In total, seven hundred CNVs were identified ranging in size from 6.1 Kb to 0.57 Mb across all autosomes, with an average size of 43.08 Kb and a median size of 15.11 Kb. By merging overlapping CNVs, we found a total of three hundred and fifty-three CNV regions (CNVRs. The length of the CNVRs ranged from 6.1 Kb to 1.45 Mb with average and median sizes of 38.49 Kb and 13.1 Kb. Collectively, 13.59 Mb of copy number variation was identified among the horses investigated and accounted for approximately 0.61% of the horse genome sequence. Five hundred and eighteen annotated genes were affected by CNVs, which corresponded to about 2.26% of all horse genes. Through the gene ontology (GO, genetic pathway analysis and comparison of CNV genes among different breeds, we found evidence that CNVs involving 7 genes may be related to the adaptation to severe environment of these plateau horses. This study is the first report of copy number variations in Chinese horses, which indicates that CNVs are ubiquitous in the horse genome and influence many biological processes of the horse. These results will be helpful not only in mapping the horse whole-genome CNVs, but also to further research for the adaption to the high altitude severe environment for plateau horses.

  16. Recurrence, submicroscopic complexity, and potential clinical relevance of copy gains detected by array CGH that are shown to be unbalanced insertions by FISH.

    Neill, Nicholas J; Ballif, Blake C; Lamb, Allen N; Parikh, Sumit; Ravnan, J Britt; Schultz, Roger A; Torchia, Beth S; Rosenfeld, Jill A; Shaffer, Lisa G

    2011-04-01

    Insertions occur when a segment of one chromosome is translocated and inserted into a new region of the same chromosome or a non-homologous chromosome. We report 71 cases with unbalanced insertions identified using array CGH and FISH in 4909 cases referred to our laboratory for array CGH and found to have copy-number abnormalities. Although the majority of insertions were non-recurrent, several recurrent unbalanced insertions were detected, including three der(Y)ins(Y;18)(q?11.2;p11.32p11.32)pat inherited from parents carrying an unbalanced insertion. The clinical significance of these recurrent rearrangements is unclear, although the small size, limited gene content, and inheritance pattern of each suggests that the phenotypic consequences may be benign. Cryptic, submicroscopic duplications were observed at or near the insertion sites in two patients, further confounding the clinical interpretation of these insertions. Using FISH, linear amplification, and array CGH, we identified a 126-kb duplicated region from 19p13.3 inserted into MECP2 at Xq28 in a patient with symptoms of Rett syndrome. Our results demonstrate that although the interpretation of most non-recurrent insertions is unclear without high-resolution insertion site characterization, the potential for an otherwise benign duplication to result in a clinically relevant outcome through the disruption of a gene necessitates the use of FISH to determine whether copy-number gains detected by array CGH represent tandem duplications or unbalanced insertions. Further follow-up testing using techniques such as linear amplification or sequencing should be used to determine gene involvement at the insertion site after FISH has identified the presence of an insertion.

  17. Integrated high-resolution array CGH and SKY analysis of homozygous deletions and other genomic alterations present in malignant mesothelioma cell lines.

    Klorin, Geula; Rozenblum, Ester; Glebov, Oleg; Walker, Robert L; Park, Yoonsoo; Meltzer, Paul S; Kirsch, Ilan R; Kaye, Frederic J; Roschke, Anna V

    2013-05-01

    High-resolution oligonucleotide array comparative genomic hybridization (aCGH) and spectral karyotyping (SKY) were applied to a panel of malignant mesothelioma (MMt) cell lines. SKY has not been applied to MMt before, and complete karyotypes are reported based on the integration of SKY and aCGH results. A whole genome search for homozygous deletions (HDs) produced the largest set of recurrent and non-recurrent HDs for MMt (52 recurrent HDs in 10 genomic regions; 36 non-recurrent HDs). For the first time, LINGO2, RBFOX1/A2BP1, RPL29, DUSP7, and CCSER1/FAM190A were found to be homozygously deleted in MMt, and some of these genes could be new tumor suppressor genes for MMt. Integration of SKY and aCGH data allowed reconstruction of chromosomal rearrangements that led to the formation of HDs. Our data imply that only with acquisition of structural and/or numerical karyotypic instability can MMt cells attain a complete loss of tumor suppressor genes located in 9p21.3, which is the most frequently homozygously deleted region. Tetraploidization is a late event in the karyotypic progression of MMt cells, after HDs in the 9p21.3 region have already been acquired. Published by Elsevier Inc.

  18. New insights in the interpretation of array-CGH: autism spectrum disorder and positive family history for intellectual disability predict the detection of pathogenic variants.

    Cappuccio, Gerarda; Vitiello, Francesco; Casertano, Alberto; Fontana, Paolo; Genesio, Rita; Bruzzese, Dario; Ginocchio, Virginia Maria; Mormile, Angela; Nitsch, Lucio; Andria, Generoso; Melis, Daniela

    2016-04-12

    Array-CGH (aCGH) is presently used into routine clinical practice for diagnosis of patients with intellectual disability (ID), multiple congenital anomalies (MCA), and autism spectrum disorder (ASD). ACGH could detect small chromosomal imbalances, copy number variations (CNVs), and closely define their size and gene content. ACGH detects pathogenic imbalances in 14-20 % of patients with ID. The aims of this study were: to establish clinical clues potentially associated with pathogenic CNVs and to identify cytogenetic indicators to predict the pathogenicity of the variants of uncertain significance (VOUS) in a large cohort of paediatric patients. We enrolled 214 patients referred for either: ID, and/or ASD and/or MCA to genetic services at the Federico II University of Naples, Department of Translational Medicine. For each patient we collected clinical and imaging data. All the patients were tested with aCGH or as first-tier test or as part of a wider diagnostic work-up. Pathologic data were detected in 65 individuals (30 %) and 46 CNVs revealed a known syndrome. The pathological CNVs were usually deletions showing the highest gene-dosage content. The positive family history for ID/ASD/MCA and ASD were good indicators for detecting pathological chromosomal rearrangements. Other clinical features as eyes anomalies, hearing loss, neurological signs, cutaneous dyscromia and endocrinological problems seem to be potential predictors of pathological CNVs. Among patients carrying VOUS we analyzed genetic features including CNVs size, presence of deletion or duplication, genic density, multiple CNVs, to clinical features. Higher gene density was found in patients affected by ID. This result suggest that higher gene content has more chances to include pathogenic gene involved and causing ID in these patients. Our study suggest the use of aCGH as first-tier test in patients with neurdevelopmental phenotypes. The inferred results have been used for building a flow-chart to be

  19. Phenotype and 244k array-CGH characterization of chromosome 13q deletions: an update of the phenotypic map of 13q21.1-qter

    Kirchhoff, Maria; Bisgaard, Anne-Marie; Stoeva, Radka

    2009-01-01

    Partial deletions of the long arm of chromosome 13 lead to variable phenotypes dependant on the size and position of the deleted region. In order to update the phenotypic map of chromosome 13q21.1-qter deletions, we applied 244k Agilent oligonucleotide-based array-CGH to determine the exact break......-genotype correlation on chromosome 13. In contrast to previous reports of carriers of 13q32 band deletions as the most seriously affected patients, we present two such individuals with long-term survival, 28 and 2.5 years....

  20. Carcinoma ex-pleomorphic adenoma derived from recurrent pleomorphic adenoma shows important difference by array CGH compared to recurrent pleomorphic adenoma without malignant transformation

    Fernanda Viviane Mariano

    Full Text Available Abstract Introduction: A key step of cancer development is the progressive accumulation of genomic changes resulting in disruption of several biological mechanisms. Carcinoma ex-pleomorphic adenoma (CXPA is an aggressive neoplasm that arises from a pleomorphic adenoma. CXPA derived from a recurrent PA (RPA has been rarely reported, and the genomic changes associated with these tumors have not yet been studied. Objective: We analyzed CXPA from RPAs and RPAs without malignant transformation using array-comparative genomic hybridization (array-CGH to identify somatic copy number alterations and affected genes. Methods: DNA samples extracted from FFPE tumors were submitted to array-CGH investigation, and data was analyzed by Nexus Copy Number Discovery Edition v.7. Results: No somatic copy number alterations were found in RPAs without malignant transformation. As for CXPA from RPA, although genomic profiles were unique for each case, we detected some chromosomal regions that appear to be preferentially affected by copy number alterations. The first case of CXPA-RPA (frankly invasive myoepithelial carcinoma showed copy number alterations affecting 1p36.33p13, 5p and chromosomes 3 and 8. The second case of CXPA-RPA (frankly invasive epithelial-myoepithelial carcinoma showed several alterations at chromosomes 3, 8, and 16, with two amplifications at 8p12p11.21 and 12q14.3q21.2. The third case of CXPA-RPA (minimally invasive epithelial-myoepithelial carcinoma exhibited amplifications at 12q13.3q14.1, 12q14.3, and 12q15. Conclusion: The occurrence of gains at chromosomes 3 and 8 and genomic amplifications at 8p and 12q, mainly those encompassing the HMGA2, MDM2, WIF1, WHSC1L1, LIRG3, CDK4 in CXAP from RPA can be a significant promotional factor in malignant transformation.

  1. Custom CGH array profiling of copy number variations (CNVs) on chromosome 6p21.32 (HLA locus) in patients with venous malformations associated with multiple sclerosis

    2010-01-01

    Background Multiple sclerosis (MS) is a complex disorder thought to result from an interaction between environmental and genetic predisposing factors which have not yet been characterised, although it is known to be associated with the HLA region on 6p21.32. Recently, a picture of chronic cerebrospinal venous insufficiency (CCSVI), consequent to stenosing venous malformation of the main extra-cranial outflow routes (VM), has been described in patients affected with MS, introducing an additional phenotype with possible pathogenic significance. Methods In order to explore the presence of copy number variations (CNVs) within the HLA locus, a custom CGH array was designed to cover 7 Mb of the HLA locus region (6,899,999 bp; chr6:29,900,001-36,800,000). Genomic DNA of the 15 patients with CCSVI/VM and MS was hybridised in duplicate. Results In total, 322 CNVs, of which 225 were extragenic and 97 intragenic, were identified in 15 patients. 234 known polymorphic CNVs were detected, the majority of these being situated in non-coding or extragenic regions. The overall number of CNVs (both extra- and intragenic) showed a robust and significant correlation with the number of stenosing VMs (Spearman: r = 0.6590, p = 0.0104; linear regression analysis r = 0.6577, p = 0.0106). The region we analysed contains 211 known genes. By using pathway analysis focused on angiogenesis and venous development, MS, and immunity, we tentatively highlight several genes as possible susceptibility factor candidates involved in this peculiar phenotype. Conclusions The CNVs contained in the HLA locus region in patients with the novel phenotype of CCSVI/VM and MS were mapped in detail, demonstrating a significant correlation between the number of known CNVs found in the HLA region and the number of CCSVI-VMs identified in patients. Pathway analysis revealed common routes of interaction of several of the genes involved in angiogenesis and immunity contained within this region. Despite the small

  2. Tiling array-CGH for the assessment of genomic similarities among synchronous unilateral and bilateral invasive breast cancer tumor pairs

    Ringnér Markus

    2008-07-01

    Full Text Available Abstract Background Today, no objective criteria exist to differentiate between individual primary tumors and intra- or intermammary dissemination respectively, in patients diagnosed with two or more synchronous breast cancers. To elucidate whether these tumors most likely arise through clonal expansion, or whether they represent individual primary tumors is of tumor biological interest and may have clinical implications. In this respect, high resolution genomic profiling may provide a more reliable approach than conventional histopathological and tumor biological factors. Methods 32 K tiling microarray-based comparative genomic hybridization (aCGH was used to explore the genomic similarities among synchronous unilateral and bilateral invasive breast cancer tumor pairs, and was compared with histopathological and tumor biological parameters. Results Based on global copy number profiles and unsupervised hierarchical clustering, five of ten (p = 1.9 × 10-5 unilateral tumor pairs displayed similar genomic profiles within the pair, while only one of eight bilateral tumor pairs (p = 0.29 displayed pair-wise genomic similarities. DNA index, histological type and presence of vessel invasion correlated with the genomic analyses. Conclusion Synchronous unilateral tumor pairs are often genomically similar, while synchronous bilateral tumors most often represent individual primary tumors. However, two independent unilateral primary tumors can develop synchronously and contralateral tumor spread can occur. The presence of an intraductal component is not informative when establishing the independence of two tumors, while vessel invasion, the presence of which was found in clustering tumor pairs but not in tumor pairs that did not cluster together, supports the clustering outcome. Our data suggest that genomically similar unilateral tumor pairs may represent a more aggressive disease that requires the addition of more severe treatment modalities, and

  3. High-resolution array CGH profiling identifies Na/K transporting ATPase interacting 2 (NKAIN2) as a predisposing candidate gene in neuroblastoma.

    Romania, Paolo; Castellano, Aurora; Surace, Cecilia; Citti, Arianna; De Ioris, Maria Antonietta; Sirleto, Pietro; De Mariano, Marilena; Longo, Luca; Boldrini, Renata; Angioni, Adriano; Locatelli, Franco; Fruci, Doriana

    2013-01-01

    Neuroblastoma (NB), the most common solid cancer in early childhood, usually occurs sporadically but also its familial occurance is known in 1-2% of NB patients. Germline mutations in the ALK and PHOX2B genes have been found in a subset of familial NBs. However, because some individuals harbouring mutations in these genes do not develop this tumor, additional genetic alterations appear to be required for NB pathogenesis. Herein, we studied an Italian family with three NB patients, two siblings and a first cousin, carrying an ALK germline-activating mutation R1192P, that was inherited from their unaffected mothers and with no mutations in the PHOX2B gene. A comparison between somatic and germline DNA copy number changes in the two affected siblings by a high resolution array-based Comparative Genomic Hybridization (CGH) analysis revealed a germline gain at NKAIN2 (Na/K transporting ATPase interacting 2) locus in one of the sibling, that was inherited from the parent who does not carry the ALK mutation. Surprisingly, NKAIN2 was expressed at high levels also in the affected sibling that lacks the genomic gain at this locus, clearly suggesting the existance of other regulatory mechanisms. High levels of NKAIN2 were detected in the MYCN-amplified NB cell lines and in the most aggressive NB lesions as well as in the peripheral blood of a large cohort of NB patients. Consistent with a role of NKAIN2 in NB development, NKAIN2 was down-regulated during all-trans retinoic acid differentiation in two NB cell lines. Taken together, these data indicate a potential role of NKAIN2 gene in NB growth and differentiation.

  4. High-resolution array CGH profiling identifies Na/K transporting ATPase interacting 2 (NKAIN2 as a predisposing candidate gene in neuroblastoma.

    Paolo Romania

    Full Text Available Neuroblastoma (NB, the most common solid cancer in early childhood, usually occurs sporadically but also its familial occurance is known in 1-2% of NB patients. Germline mutations in the ALK and PHOX2B genes have been found in a subset of familial NBs. However, because some individuals harbouring mutations in these genes do not develop this tumor, additional genetic alterations appear to be required for NB pathogenesis. Herein, we studied an Italian family with three NB patients, two siblings and a first cousin, carrying an ALK germline-activating mutation R1192P, that was inherited from their unaffected mothers and with no mutations in the PHOX2B gene. A comparison between somatic and germline DNA copy number changes in the two affected siblings by a high resolution array-based Comparative Genomic Hybridization (CGH analysis revealed a germline gain at NKAIN2 (Na/K transporting ATPase interacting 2 locus in one of the sibling, that was inherited from the parent who does not carry the ALK mutation. Surprisingly, NKAIN2 was expressed at high levels also in the affected sibling that lacks the genomic gain at this locus, clearly suggesting the existance of other regulatory mechanisms. High levels of NKAIN2 were detected in the MYCN-amplified NB cell lines and in the most aggressive NB lesions as well as in the peripheral blood of a large cohort of NB patients. Consistent with a role of NKAIN2 in NB development, NKAIN2 was down-regulated during all-trans retinoic acid differentiation in two NB cell lines. Taken together, these data indicate a potential role of NKAIN2 gene in NB growth and differentiation.

  5. Application of oligonucleotide array CGH to the simultaneous detection of a deletion in the nuclear TK2 gene and mtDNA depletion.

    Zhang, Shulin; Li, Fang-Yuan; Bass, Harold N; Pursley, Amber; Schmitt, Eric S; Brown, Blaire L; Brundage, Ellen K; Mardach, Rebecca; Wong, Lee-Jun

    2010-01-01

    Thymidine kinase 2 (TK2), encoded by the TK2 gene on chromosome 16q22, is one of the deoxyribonucleoside kinases responsible for the maintenance of mitochondrial deoxyribonucleotide pools. Defects in TK2 mainly cause a myopathic form of the mitochondrial DNA depletion syndrome (MDDS). Currently, only point mutations and small insertions and deletions have been reported in TK2 gene; gross rearrangements of TK2 gene and possible hepatic involvement in patients with TK2 mutations have not been described. We report a non-consanguineous Jordanian family with three deceased siblings due to mtDNA depletion. Sequence analysis of the father detected a heterozygous c.761T>A (p.I254N) mutation in his TK2 gene; however, point mutations in the mother were not detected. Subsequent gene dosage analysis using oligonucleotide array CGH identified an intragenic approximately 5.8-kb deletion encompassing the 5'UTR to intron 2 of her TK2 gene. Sequence analysis confirmed that the deletion spans c.1-495 to c.283-2899 of the TK2 gene (nucleotide 65,136,256-65,142,086 of chromosome 16). Analysis of liver and muscle specimens from one of the deceased infants in this family revealed compound heterozygosity for the paternal point mutation and maternal intragenic deletion. In addition, a significant reduction of the mtDNA content in liver and muscle was detected (10% and 20% of age- and tissue-matched controls, respectively). Prenatal diagnosis was performed in the third pregnancy. The fetus was found to carry both the point mutation and the deletion. This child died 6months after birth due to myopathy. A serum specimen demonstrated elevated liver transaminases in two of the infants from whom results were available. This report expands the mutation spectrum associated with TK2 deficiency. While the myopathic form of MDDS appears to be the main phenotype of TK2 mutations, liver dysfunction may also be a part of the mitochondrial depletion syndrome caused by TK2 gene defects.

  6. Programmable Array Logic Design

    Demon Handoyo; Djen Djen Djainal

    2007-01-01

    Good digital circuit design that part of a complex system, often becoming a separate problem. To produce finishing design according to wanted performance is often given on to considerations which each other confuse, hence thereby analyse optimization become important in this case. To realization is made design logic program, the first are determined global diagram block, then are decided contents of these block diagram, and then determined its interconnection in the form of logic expression, continued with election of component. These steps are done to be obtained the design with low price, easy in its interconnection, minimal volume, low power and certainty god work. (author)

  7. Development of a novel ozone- and photo-stable HyPer5 red fluorescent dye for array CGH and microarray gene expression analysis with consistent performance irrespective of environmental conditions

    Kille Peter

    2008-11-01

    Full Text Available Abstract Background Array-based comparative genomic hybridization (CGH and gene expression profiling have become vital techniques for identifying molecular defects underlying genetic diseases. Regardless of the microarray platform, cyanine dyes (Cy3 and Cy5 are one of the most widely used fluorescent dye pairs for microarray analysis owing to their brightness and ease of incorporation, enabling high level of assay sensitivity. However, combining both dyes on arrays can become problematic during summer months when ozone levels rise to near 25 parts per billion (ppb. Under such conditions, Cy5 is known to rapidly degrade leading to loss of signal from either "homebrew" or commercial arrays. Cy5 can also suffer disproportionately from dye photobleaching resulting in distortion of (Cy5/Cy3 ratios used in copy number analysis. Our laboratory has been active in fluorescent dye research to find a suitable alternative to Cy5 that is stable to ozone and resistant to photo-bleaching. Here, we report on the development of such a dye, called HyPer5, and describe its' exceptional ozone and photostable properties on microarrays. Results Our results show HyPer5 signal to be stable to high ozone levels. Repeated exposure of mouse arrays hybridized with HyPer5-labeled cDNA to 300 ppb ozone at 5, 10 and 15 minute intervals resulted in no signal loss from the dye. In comparison, Cy5 arrays showed a dramatic 80% decrease in total signal during the same interval. Photobleaching experiments show HyPer5 to be resistant to light induced damage with 3- fold improvement in dye stability over Cy5. In high resolution array CGH experiments, HyPer5 is demonstrated to detect chromosomal aberrations at loci 2p21-16.3 and 15q26.3-26.2 from three patient sample using bacterial artificial chromosome (BAC arrays. The photostability of HyPer5 is further documented by repeat array scanning without loss of detection. Additionally, HyPer5 arrays are shown to preserve sensitivity and

  8. Array-based gene expression, CGH and tissue data defines a 12q24 gain in neuroblastic tumors with prognostic implication

    Kilpinen Sami

    2010-05-01

    Full Text Available Abstract Background Neuroblastoma has successfully served as a model system for the identification of neuroectoderm-derived oncogenes. However, in spite of various efforts, only a few clinically useful prognostic markers have been found. Here, we present a framework, which integrates DNA, RNA and tissue data to identify and prioritize genetic events that represent clinically relevant new therapeutic targets and prognostic biomarkers for neuroblastoma. Methods A single-gene resolution aCGH profiling was integrated with microarray-based gene expression profiling data to distinguish genetic copy number alterations that were strongly associated with transcriptional changes in two neuroblastoma cell lines. FISH analysis using a hotspot tumor tissue microarray of 37 paraffin-embedded neuroblastoma samples and in silico data mining for gene expression information obtained from previously published studies including up to 445 healthy nervous system samples and 123 neuroblastoma samples were used to evaluate the clinical significance and transcriptional consequences of the detected alterations and to identify subsequently activated gene(s. Results In addition to the anticipated high-level amplification and subsequent overexpression of MYCN, MEIS1, CDK4 and MDM2 oncogenes, the aCGH analysis revealed numerous other genetic alterations, including microamplifications at 2p and 12q24.11. Most interestingly, we identified and investigated the clinical relevance of a previously poorly characterized amplicon at 12q24.31. FISH analysis showed low-level gain of 12q24.31 in 14 of 33 (42% neuroblastomas. Patients with the low-level gain had an intermediate prognosis in comparison to patients with MYCN amplification (poor prognosis and to those with no MYCN amplification or 12q24.31 gain (good prognosis (P = 0.001. Using the in silico data mining approach, we identified elevated expression of five genes located at the 12q24.31 amplicon in neuroblastoma (DIABLO, ZCCHC

  9. Design of circular differential microphone arrays

    Benesty, Jacob; Cohen, Israel

    2015-01-01

    Recently, we proposed a completely novel and efficient way to design differential beamforming algorithms for linear microphone arrays. Thanks to this very flexible approach, any order of differential arrays can be designed. Moreover, they can be made robust against white noise amplification, which is the main inconvenience in these types of arrays. The other well-known problem with linear arrays is that electronic steering is not really feasible.  In this book, we extend all these fundamental ideas to circular microphone arrays and show that we can design small and compact differential arrays of any order that can be electronically steered in many different directions and offer a good degree of control of the white noise amplification problem, high directional gain, and frequency-independent response. We also present a number of practical examples, demonstrating that differential beamforming with circular microphone arrays is likely one of the best candidates for applications involving speech enhancement (i....

  10. Small regions of overlapping deletions on 6q26 in human astrocytic tumours identified using chromosome 6 tile path array CGH

    Ichimura, Koichi; Mungall, Andrew J; Fiegler, Heike; Pearson, Danita M.; Dunham, Ian; Carter, Nigel P; Collins, V. Peter

    2009-01-01

    Deletions of chromosome 6 are a common abnormality in diverse human malignancies including astrocytic tumours, suggesting the presence of tumour suppressor genes (TSG). In order to help identify candidate TSGs, we have constructed a chromosome 6 tile path microarray. The array contains 1780 clones (778 PACs and 1002 BACs) that cover 98.3% of the published chromosome 6 sequences. A total of 104 adult astrocytic tumours (10 diffuse astrocytomas, 30 anaplastic astrocytomas (AA), 64 glioblastomas (GB)) were analysed using this array. Single copy number change was successfully detected and the result was in general concordant with a microsatellite analysis. The pattern of copy number change was complex with multiple interstitial deletions/gains. However, a predominance of telomeric 6q deletions was seen. Two small common and overlapping regions of deletion at 6q26 were identified. One was 1002 kb in size and contained PACRG and QKI, while the second was 199 kb and harbours a single gene, ARID1B. The data show that the chromosome 6 tile path array is useful in mapping copy number changes with high resolution and accuracy. We confirmed the high frequency of chromosome 6 deletions in AA and GB, and identified two novel commonly deleted regions that may harbour TSGs. PMID:16205629

  11. arrayCGHbase: an analysis platform for comparative genomic hybridization microarrays

    Moreau Yves

    2005-05-01

    Full Text Available Abstract Background The availability of the human genome sequence as well as the large number of physically accessible oligonucleotides, cDNA, and BAC clones across the entire genome has triggered and accelerated the use of several platforms for analysis of DNA copy number changes, amongst others microarray comparative genomic hybridization (arrayCGH. One of the challenges inherent to this new technology is the management and analysis of large numbers of data points generated in each individual experiment. Results We have developed arrayCGHbase, a comprehensive analysis platform for arrayCGH experiments consisting of a MIAME (Minimal Information About a Microarray Experiment supportive database using MySQL underlying a data mining web tool, to store, analyze, interpret, compare, and visualize arrayCGH results in a uniform and user-friendly format. Following its flexible design, arrayCGHbase is compatible with all existing and forthcoming arrayCGH platforms. Data can be exported in a multitude of formats, including BED files to map copy number information on the genome using the Ensembl or UCSC genome browser. Conclusion ArrayCGHbase is a web based and platform independent arrayCGH data analysis tool, that allows users to access the analysis suite through the internet or a local intranet after installation on a private server. ArrayCGHbase is available at http://medgen.ugent.be/arrayCGHbase/.

  12. Flat-plate photovoltaic array design optimization

    Ross, R. G., Jr.

    1980-01-01

    An analysis is presented which integrates the results of specific studies in the areas of photovoltaic structural design optimization, optimization of array series/parallel circuit design, thermal design optimization, and optimization of environmental protection features. The analysis is based on minimizing the total photovoltaic system life-cycle energy cost including repair and replacement of failed cells and modules. This approach is shown to be a useful technique for array optimization, particularly when time-dependent parameters such as array degradation and maintenance are involved.

  13. Array-CGH in patients with Kabuki-like phenotype: identification of two patients with complex rearrangements including 2q37 deletions and no other recurrent aberration.

    Cuscó, Ivon; del Campo, Miguel; Vilardell, Mireia; González, Eva; Gener, Blanca; Galán, Enrique; Toledo, Laura; Pérez-Jurado, Luis A

    2008-04-11

    Kabuki syndrome (KS) is a multiple congenital anomaly syndrome characterized by specific facial features, mild to moderate mental retardation, postnatal growth delay, skeletal abnormalities, and unusual dermatoglyphic patterns with prominent fingertip pads. A 3.5 Mb duplication at 8p23.1-p22 was once reported as a specific alteration in KS but has not been confirmed in other patients. The molecular basis of KS remains unknown. We have studied 16 Spanish patients with a clinical diagnosis of KS or KS-like to search for genomic imbalances using genome-wide array technologies. All putative rearrangements were confirmed by FISH, microsatellite markers and/or MLPA assays, which also determined whether the imbalance was de novo or inherited. No duplication at 8p23.1-p22 was observed in our patients. We detected complex rearrangements involving 2q in two patients with Kabuki-like features: 1) a de novo inverted duplication of 11 Mb with a 4.5 Mb terminal deletion, and 2) a de novo 7.2 Mb-terminal deletion in a patient with an additional de novo 0.5 Mb interstitial deletion in 16p. Additional copy number variations (CNV), either inherited or reported in normal controls, were identified and interpreted as polymorphic variants. No specific CNV was significantly increased in the KS group. Our results further confirmed that genomic duplications of 8p23 region are not a common cause of KS and failed to detect other recurrent rearrangement causing this disorder. The detection of two patients with 2q37 deletions suggests that there is a phenotypic overlap between the two conditions, and screening this region in the Kabuki-like patients should be considered.

  14. Genomic profiling of plasmablastic lymphoma using array comparative genomic hybridization (aCGH: revealing significant overlapping genomic lesions with diffuse large B-cell lymphoma

    Lu Xin-Yan

    2009-11-01

    Full Text Available Abstract Background Plasmablastic lymphoma (PL is a subtype of diffuse large B-cell lymphoma (DLBCL. Studies have suggested that tumors with PL morphology represent a group of neoplasms with clinopathologic characteristics corresponding to different entities including extramedullary plasmablastic tumors associated with plasma cell myeloma (PCM. The goal of the current study was to evaluate the genetic similarities and differences among PL, DLBCL (AIDS-related and non AIDS-related and PCM using array-based comparative genomic hybridization. Results Examination of genomic data in PL revealed that the most frequent segmental gain (> 40% include: 1p36.11-1p36.33, 1p34.1-1p36.13, 1q21.1-1q23.1, 7q11.2-7q11.23, 11q12-11q13.2 and 22q12.2-22q13.3. This correlated with segmental gains occurring in high frequency in DLBCL (AIDS-related and non AIDS-related cases. There were some segmental gains and some segmental loss that occurred in PL but not in the other types of lymphoma suggesting that these foci may contain genes responsible for the differentiation of this lymphoma. Additionally, some segmental gains and some segmental loss occurred only in PL and AIDS associated DLBCL suggesting that these foci may be associated with HIV infection. Furthermore, some segmental gains and some segmental loss occurred only in PL and PCM suggesting that these lesions may be related to plasmacytic differentiation. Conclusion To the best of our knowledge, the current study represents the first genomic exploration of PL. The genomic aberration pattern of PL appears to be more similar to that of DLBCL (AIDS-related or non AIDS-related than to PCM. Our findings suggest that PL may remain best classified as a subtype of DLBCL at least at the genome level.

  15. Designing Flat-Plate Photovoltaic Arrays

    Ross, R. G., Jr.

    1984-01-01

    Report presents overview of state of art in design techniques for flat-plate solar photovoltaic modules and arrays. Paper discusses design requirements, design analyses, and test methods identified and developed for this technology over past several years in effort to reduce cost and improve utility and reliability for broad spectrum of terrestrial applications.

  16. Detection and precise mapping of germline rearrangements in BRCA1, BRCA2, MSH2, and MLH1 using zoom-in array comparative genomic hybridization (aCGH)

    Staaf, Johan; Törngren, Therese; Rambech, Eva

    2008-01-01

    Disease-predisposing germline mutations in cancer susceptibility genes may consist of large genomic rearrangements that are challenging to detect and characterize using standard PCR-based mutation screening methods. Here, we describe a custom-made zoom-in microarray comparative genomic hybridizat......Disease-predisposing germline mutations in cancer susceptibility genes may consist of large genomic rearrangements that are challenging to detect and characterize using standard PCR-based mutation screening methods. Here, we describe a custom-made zoom-in microarray comparative genomic...... deletions or duplications occurring in BRCA1 (n=11), BRCA2 (n=2), MSH2 (n=7), or MLH1 (n=9). Additionally, we demonstrate its applicability for uncovering complex somatic rearrangements, exemplified by zoom-in analysis of the PTEN and CDKN2A loci in breast cancer cells. The sizes of rearrangements ranged...... from several 100 kb, including large flanking regions, to rearrangements, allowing convenient design...

  17. Study and Design of Differential Microphone Arrays

    Benesty, Jacob

    2013-01-01

    Microphone arrays have attracted a lot of interest over the last few decades since they have the potential to solve many important problems such as noise reduction/speech enhancement, source separation, dereverberation, spatial sound recording, and source localization/tracking, to name a few. However, the design and implementation of microphone arrays with beamforming algorithms is not a trivial task when it comes to processing broadband signals such as speech. Indeed, in most sensor arrangements, the beamformer tends to have a frequency-dependent response. One exception, perhaps, is the family of differential microphone arrays (DMAs) that have the promise to form frequency-independent responses. Moreover, they have the potential to attain high directional gains with small and compact apertures. As a result, this type of microphone arrays has drawn much research and development attention recently. This book is intended to provide a systematic study of DMAs from a signal processing perspective. The primary obj...

  18. MYC and MYCN amplification can be reliably assessed by aCGH in medulloblastoma.

    Bourdeaut, Franck; Grison, Camille; Maurage, Claude-Alain; Laquerriere, Annie; Vasiljevic, Alexandre; Delisle, Marie-Bernadette; Michalak, Sophie; Figarella-Branger, Dominique; Doz, François; Richer, Wilfrid; Pierron, Gaelle; Miquel, Catherine; Delattre, Olivier; Couturier, Jérôme

    2013-04-01

    As prognostic factors, MYC and MYCN amplifications are routinely assessed in medulloblastomas. Fluorescence in situ hybridization (FISH) is currently considered as the technique of reference. Recently, array comparative genomic hybridization (aCGH) has been developed as an alternative technique to evaluate genomic abnormalities in other tumor types; however, this technique has not been widely adopted as a replacement for FISH in medulloblastoma. In this study, 34 tumors were screened by both FISH and aCGH. In all cases showing amplification by FISH, aCGH also unambiguously revealed the abnormality. The aCGH technique was also performed on tumors showing no amplification by FISH, and the absence of amplification was confirmed in all cases. Interestingly, one tumor showed a subclonal MYC amplification by FISH. This subclonal amplification was observed in approximately 20% of tumor cells and was clearly evident on aCGH. In conclusion, our analysis confirms that aCGH is as safe as FISH for the detection of MYC/MYCN gene amplification. Given its cost efficiency in comparison to two FISH tests and the global genomic information additionally provided by an aCGH experiment, this reproducible technique can be safely retained as an alternative to FISH for routine investigation of medulloblastoma. Copyright © 2013 Elsevier Inc. All rights reserved.

  19. Oligo-based High-resolution aCGH Analysis Enhances Routine Cytogenetic Diagnostics in Haematological Malignancies.

    Kjeldsen, Eigil

    2015-01-01

    The purpose of the present study was to evaluate the detection rate of genomic aberrations in haematological malignancies using oligobased array-CGH (oaCGH) analysis in combination with karyotyping and fluorescence in situ hybridization (FISH) analyses, and its feasibility in a clinical pragmatic approach. The 4x180K Cancer Cytochip array was applied in 96 patients with various haematological malignancies in a prospective setting and in 41 acute myeloid leukemia (AML) patients retrospectively. Combined use of oaCGH analysis and karyotyping improved the overall detection rate in comparison to karyotyping-alone and vice versa. In cases with normal karyotypes oaCGH analysis detected genomic aberrations in 66% (39/60) of cases. In the group of simple karyotypes oaCGH analysis extended karyotypic findings in 39% (12/31) while oaCGH analysis extended the karyotypic findings in 89% (39/44) of cases with complex karyotypes. In 7% (5/75) of cases oaCGH analysis failed in detecting the observed abnormalities by karyotyping. oaCGH analysis is a valuable asset in routine cytogenetics of haematological malignancies. Copyright© 2015, International Institute of Anticancer Research (Dr. John G. Delinasios), All rights reserved.

  20. Simulation of sparse matrix array designs

    Boehm, Rainer; Heckel, Thomas

    2018-04-01

    Matrix phased array probes are becoming more prominently used in industrial applications. The main drawbacks, using probes incorporating a very large number of transducer elements, are needed for an appropriate cabling and an ultrasonic device offering many parallel channels. Matrix arrays designed for extended functionality feature at least 64 or more elements. Typical arrangements are square matrices, e.g., 8 by 8 or 11 by 11 or rectangular matrixes, e.g., 8 by 16 or 10 by 12 to fit a 128-channel phased array system. In some phased array systems, the number of simultaneous active elements is limited to a certain number, e.g., 32 or 64. Those setups do not allow running the probe with all elements active, which may cause a significant change in the directivity pattern of the resulting sound beam. When only a subset of elements can be used during a single acquisition, different strategies may be applied to collect enough data for rebuilding the missing information from the echo signal. Omission of certain elements may be one approach, overlay of subsequent shots with different active areas may be another one. This paper presents the influence of a decreased number of active elements on the sound field and their distribution on the array. Solutions using subsets with different element activity patterns on matrix arrays and their advantages and disadvantages concerning the sound field are evaluated using semi-analytical simulation tools. Sound field criteria are discussed, which are significant for non-destructive testing results and for the system setup.

  1. A novel method to design sparse linear arrays for ultrasonic phased array.

    Yang, Ping; Chen, Bin; Shi, Ke-Ren

    2006-12-22

    In ultrasonic phased array testing, a sparse array can increase the resolution by enlarging the aperture without adding system complexity. Designing a sparse array involves choosing the best or a better configuration from a large number of candidate arrays. We firstly designed sparse arrays by using a genetic algorithm, but found that the arrays have poor performance and poor consistency. So, a method based on the Minimum Redundancy Linear Array was then adopted. Some elements are determined by the minimum-redundancy array firstly in order to ensure spatial resolution and then a genetic algorithm is used to optimize the remaining elements. Sparse arrays designed by this method have much better performance and consistency compared to the arrays designed only by a genetic algorithm. Both simulation and experiment confirm the effectiveness.

  2. Conformal array design on arbitrary polygon surface with transformation optics

    Deng, Li, E-mail: dengl@bupt.edu.cn; Hong, Weijun, E-mail: hongwj@bupt.edu.cn; Zhu, Jianfeng; Peng, Biao; Li, Shufang [Beijing Key Laboratory of Network System Architecture and Convergence, School of Information and Communication Engineering, Beijing University of Posts and Telecommunications, 100876 Beijing (China); Wu, Yongle, E-mail: wuyongle138@gmail.com [Beijing Key Laboratory of Work Safety Intelligent Monitoring, School of Electronic Engineering, Beijing University of Posts and Telecommunications, 100876 Beijing (China)

    2016-06-15

    A transformation-optics based method to design a conformal antenna array on an arbitrary polygon surface is proposed and demonstrated in this paper. This conformal antenna array can be adjusted to behave equivalently as a uniformly spaced linear array by applying an appropriate transformation medium. An typical example of general arbitrary polygon conformal arrays, not limited to circular array, is presented, verifying the proposed approach. In summary, the novel arbitrary polygon surface conformal array can be utilized in array synthesis and beam-forming, maintaining all benefits of linear array.

  3. Conformal array design on arbitrary polygon surface with transformation optics

    Deng, Li; Hong, Weijun; Zhu, Jianfeng; Peng, Biao; Li, Shufang; Wu, Yongle

    2016-01-01

    A transformation-optics based method to design a conformal antenna array on an arbitrary polygon surface is proposed and demonstrated in this paper. This conformal antenna array can be adjusted to behave equivalently as a uniformly spaced linear array by applying an appropriate transformation medium. An typical example of general arbitrary polygon conformal arrays, not limited to circular array, is presented, verifying the proposed approach. In summary, the novel arbitrary polygon surface conformal array can be utilized in array synthesis and beam-forming, maintaining all benefits of linear array.

  4. Design & fabrication of cantilever array biosensors

    Boisen, Anja; Thundat, T

    2009-01-01

    Surface immobilization of functional receptors on microfabricated cantilever arrays offers a new paradigm for the development of biosensors based on nanomechanics. Microcantilever-based systems are capable of real-time, multiplexed detection of unlabeled disease markers in extremely small volumes......, electronic processing, and even local telemetry on a single chip have the potential of satisfying the need for highly sensitive and selective multiple-target detection in very small samples. Here we will review the design and fabrication process of cantilever-based biosensors....

  5. ADaCGH: A parallelized web-based application and R package for the analysis of aCGH data.

    Ramón Díaz-Uriarte

    Full Text Available BACKGROUND: Copy number alterations (CNAs in genomic DNA have been associated with complex human diseases, including cancer. One of the most common techniques to detect CNAs is array-based comparative genomic hybridization (aCGH. The availability of aCGH platforms and the need for identification of CNAs has resulted in a wealth of methodological studies. METHODOLOGY/PRINCIPAL FINDINGS: ADaCGH is an R package and a web-based application for the analysis of aCGH data. It implements eight methods for detection of CNAs, gains and losses of genomic DNA, including all of the best performing ones from two recent reviews (CBS, GLAD, CGHseg, HMM. For improved speed, we use parallel computing (via MPI. Additional information (GO terms, PubMed citations, KEGG and Reactome pathways is available for individual genes, and for sets of genes with altered copy numbers. CONCLUSIONS/SIGNIFICANCE: ADACGH represents a qualitative increase in the standards of these types of applications: a all of the best performing algorithms are included, not just one or two; b we do not limit ourselves to providing a thin layer of CGI on top of existing BioConductor packages, but instead carefully use parallelization, examining different schemes, and are able to achieve significant decreases in user waiting time (factors up to 45x; c we have added functionality not currently available in some methods, to adapt to recent recommendations (e.g., merging of segmentation results in wavelet-based and CGHseg algorithms; d we incorporate redundancy, fault-tolerance and checkpointing, which are unique among web-based, parallelized applications; e all of the code is available under open source licenses, allowing to build upon, copy, and adapt our code for other software projects.

  6. Phased-array design for MST and ST radars

    Ecklund, W. L.

    1986-01-01

    All of the existing radar systems fully dedicated to clear-air radar studies use some type of phased-array antennas. The effects of beam-steering techniques including feed networks and phase shifters; sidelobe control; ground-clutter suppression; low altitude coverage; arrays with integrated radiating elements and feed networks; analysis of coaxial-collinear antennas; use of arrays with multiple beams; and array testing and measure on structural design of the antenna are discussed.

  7. Design of a trichromatic cone array.

    Patrick Garrigan

    2010-02-01

    Full Text Available Cones with peak sensitivity to light at long (L, medium (M and short (S wavelengths are unequal in number on the human retina: S cones are rare (<10% while increasing in fraction from center to periphery, and the L/M cone proportions are highly variable between individuals. What optical properties of the eye, and statistical properties of natural scenes, might drive this organization? We found that the spatial-chromatic structure of natural scenes was largely symmetric between the L, M and S sensitivity bands. Given this symmetry, short wavelength attenuation by ocular media gave L/M cones a modest signal-to-noise advantage, which was amplified, especially in the denser central retina, by long-wavelength accommodation of the lens. Meanwhile, total information represented by the cone mosaic remained relatively insensitive to L/M proportions. Thus, the observed cone array design along with a long-wavelength accommodated lens provides a selective advantage: it is maximally informative.

  8. Design of two easily-testable VLSI array multipliers

    Ferguson, J.; Shen, J.P.

    1983-01-01

    Array multipliers are well-suited to VLSI implementation because of the regularity in their iterative structure. However, most VLSI circuits are very difficult to test. This paper shows that, with appropriate cell design, array multipliers can be designed to be very easily testable. An array multiplier is called c-testable if all its adder cells can be exhaustively tested while requiring only a constant number of test patterns. The testability of two well-known array multiplier structures are studied. The conventional design of the carry-save array multipler is shown to be not c-testable. However, a modified design, using a modified adder cell, is generated and shown to be c-testable and requires only 16 test patterns. Similar results are obtained for the baugh-wooley two's complement array multiplier. A modified design of the baugh-wooley array multiplier is shown to be c-testable and requires 55 test patterns. The implementation of a practical c-testable 16*16 array multiplier is also presented. 10 references.

  9. A multi-sample based method for identifying common CNVs in normal human genomic structure using high-resolution aCGH data.

    Chihyun Park

    Full Text Available BACKGROUND: It is difficult to identify copy number variations (CNV in normal human genomic data due to noise and non-linear relationships between different genomic regions and signal intensity. A high-resolution array comparative genomic hybridization (aCGH containing 42 million probes, which is very large compared to previous arrays, was recently published. Most existing CNV detection algorithms do not work well because of noise associated with the large amount of input data and because most of the current methods were not designed to analyze normal human samples. Normal human genome analysis often requires a joint approach across multiple samples. However, the majority of existing methods can only identify CNVs from a single sample. METHODOLOGY AND PRINCIPAL FINDINGS: We developed a multi-sample-based genomic variations detector (MGVD that uses segmentation to identify common breakpoints across multiple samples and a k-means-based clustering strategy. Unlike previous methods, MGVD simultaneously considers multiple samples with different genomic intensities and identifies CNVs and CNV zones (CNVZs; CNVZ is a more precise measure of the location of a genomic variant than the CNV region (CNVR. CONCLUSIONS AND SIGNIFICANCE: We designed a specialized algorithm to detect common CNVs from extremely high-resolution multi-sample aCGH data. MGVD showed high sensitivity and a low false discovery rate for a simulated data set, and outperformed most current methods when real, high-resolution HapMap datasets were analyzed. MGVD also had the fastest runtime compared to the other algorithms evaluated when actual, high-resolution aCGH data were analyzed. The CNVZs identified by MGVD can be used in association studies for revealing relationships between phenotypes and genomic aberrations. Our algorithm was developed with standard C++ and is available in Linux and MS Windows format in the STL library. It is freely available at: http://embio.yonsei.ac.kr/~Park/mgvd.php.

  10. Design of a new electrode array for cochlear implants

    Kha, H.; Chen, B.

    2010-01-01

    Full text: This study aims to design a new electrode array which can be precisely located beneath the basilar membrane within the cochlear scala tympani. This placement of the electrode array is beneficial for increasing the effectiveness of the electrical stimulation of the audi tory nerves and maximising the growth factors delivered into the cochlea for regenerating the progressively lost auditory neurons, thereby significantly improving performance of the cochlear implant systems. Methods The design process involved two steps. First, the biocom patible nitinol-based shape memory alloy, of which mechanical deformation can be controlled using electrical cUTents/fields act vated by body temperature, was selected. Second, five different designs of the electrode array with embedded nitinol actuators were studied (Table I). The finite element method was employed to predict final positions of these electrode arrays. Results The electrode array with three 6 mm actuators at 2-8, 8-J4 and 14-20 mm from the tip (Fig. I) was found to be located most closely to the basilar membrane, compared with those in the other four cases. Conclusions A new nitinol cochlear implant electrode array with three embedded nitinol actuators has been designed. This electrode array is expected to be located beneath the basilar membrane for maximising the delivery of growth factors. Future research will involve the manufacturing of a prototype of this electrode array for use in insertion experiments and neurotrophin release tests.

  11. Multispectral linear array (MLA) focal plane mechanical and thermal design

    Mitchell, A. S.; Kaminski, E. F.

    1982-01-01

    The mechanical and thermal design of an integrated focal plane subsystem of a Multispectral Linear Array (MLA) instrument is discussed in terms of focal-plane alignment, thermoelastic performance, and thermal requirements. The modular construction and thermal control of the focal plane array are discussed.

  12. Probe design for expression arrays using OligoWiz

    Wernersson, Rasmus

    2014-01-01

    Since all measurements from a DNA microarray is dependant on the probes used, a good choice of probes is of vital importa nce when designing custom micro-arrays. This chapter describes how to de sign expression arrays using the “ OligoWiz ” software suite. The general desired features of good...... probes and the issues which probe design must address are introduced and a conceptual (rather than mathematical) description of how OligoWiz scores the quality of th e potential probes is presented. This is followed by a detailed step-by-step guide to designing expression arrays with OligoWiz....

  13. Challenging aspects of contemporary cochlear implant electrode array design.

    Mistrík, Pavel; Jolly, Claude; Sieber, Daniel; Hochmair, Ingeborg

    2017-12-01

    A design comparison of current perimodiolar and lateral wall electrode arrays of the cochlear implant (CI) is provided. The focus is on functional features such as acoustic frequency coverage and tonotopic mapping, battery consumption and dynamic range. A traumacity of their insertion is also evaluated. Review of up-to-date literature. Perimodiolar electrode arrays are positioned in the basal turn of the cochlea near the modiolus. They are designed to initiate the action potential in the proximity to the neural soma located in spiral ganglion. On the other hand, lateral wall electrode arrays can be inserted deeper inside the cochlea, as they are located along the lateral wall and such insertion trajectory is less traumatic. This class of arrays targets primarily surviving neural peripheral processes. Due to their larger insertion depth, lateral wall arrays can deliver lower acoustic frequencies in manner better corresponding to cochlear tonotopicity. In fact, spiral ganglion sections containing auditory nerve fibres tuned to low acoustic frequencies are located deeper than 1 and half turn inside the cochlea. For this reason, a significant frequency mismatch might be occurring for apical electrodes in perimodiolar arrays, detrimental to speech perception. Tonal languages such as Mandarin might be therefore better treated with lateral wall arrays. On the other hand, closer proximity to target tissue results in lower psychophysical threshold levels for perimodiolar arrays. However, the maximal comfort level is also lower, paradoxically resulting in narrower dynamic range than that of lateral wall arrays. Battery consumption is comparable for both types of arrays. Lateral wall arrays are less likely to cause trauma to cochlear structures. As the current trend in cochlear implantation is the maximal protection of residual acoustic hearing, the lateral wall arrays seem more suitable for hearing preservation CI surgeries. Future development could focus on combining the

  14. APRON: A Cellular Processor Array Simulation and Hardware Design Tool

    Barr, David R. W.; Dudek, Piotr

    2009-12-01

    We present a software environment for the efficient simulation of cellular processor arrays (CPAs). This software (APRON) is used to explore algorithms that are designed for massively parallel fine-grained processor arrays, topographic multilayer neural networks, vision chips with SIMD processor arrays, and related architectures. The software uses a highly optimised core combined with a flexible compiler to provide the user with tools for the design of new processor array hardware architectures and the emulation of existing devices. We present performance benchmarks for the software processor array implemented on standard commodity microprocessors. APRON can be configured to use additional processing hardware if necessary and can be used as a complete graphical user interface and development environment for new or existing CPA systems, allowing more users to develop algorithms for CPA systems.

  15. APRON: A Cellular Processor Array Simulation and Hardware Design Tool

    David R. W. Barr

    2009-01-01

    Full Text Available We present a software environment for the efficient simulation of cellular processor arrays (CPAs. This software (APRON is used to explore algorithms that are designed for massively parallel fine-grained processor arrays, topographic multilayer neural networks, vision chips with SIMD processor arrays, and related architectures. The software uses a highly optimised core combined with a flexible compiler to provide the user with tools for the design of new processor array hardware architectures and the emulation of existing devices. We present performance benchmarks for the software processor array implemented on standard commodity microprocessors. APRON can be configured to use additional processing hardware if necessary and can be used as a complete graphical user interface and development environment for new or existing CPA systems, allowing more users to develop algorithms for CPA systems.

  16. Oligonucleotide arrays vs. metaphase-comparative genomic hybridisation and BAC arrays for single-cell analysis: first applications to preimplantation genetic diagnosis for Robertsonian translocation carriers.

    Ramos, Laia; del Rey, Javier; Daina, Gemma; García-Aragonés, Manel; Armengol, Lluís; Fernandez-Encinas, Alba; Parriego, Mònica; Boada, Montserrat; Martinez-Passarell, Olga; Martorell, Maria Rosa; Casagran, Oriol; Benet, Jordi; Navarro, Joaquima

    2014-01-01

    Comprehensive chromosome analysis techniques such as metaphase-Comparative Genomic Hybridisation (CGH) and array-CGH are available for single-cell analysis. However, while metaphase-CGH and BAC array-CGH have been widely used for Preimplantation Genetic Diagnosis, oligonucleotide array-CGH has not been used in an extensive way. A comparison between oligonucleotide array-CGH and metaphase-CGH has been performed analysing 15 single fibroblasts from aneuploid cell-lines and 18 single blastomeres from human cleavage-stage embryos. Afterwards, oligonucleotide array-CGH and BAC array-CGH were also compared analysing 16 single blastomeres from human cleavage-stage embryos. All three comprehensive analysis techniques provided broadly similar cytogenetic profiles; however, non-identical profiles appeared when extensive aneuploidies were present in a cell. Both array techniques provided an optimised analysis procedure and a higher resolution than metaphase-CGH. Moreover, oligonucleotide array-CGH was able to define extra segmental imbalances in 14.7% of the blastomeres and it better determined the specific unbalanced chromosome regions due to a higher resolution of the technique (≈ 20 kb). Applicability of oligonucleotide array-CGH for Preimplantation Genetic Diagnosis has been demonstrated in two cases of Robertsonian translocation carriers 45,XY,der(13;14)(q10;q10). Transfer of euploid embryos was performed in both cases and pregnancy was achieved by one of the couples. This is the first time that an oligonucleotide array-CGH approach has been successfully applied to Preimplantation Genetic Diagnosis for balanced chromosome rearrangement carriers.

  17. Oligonucleotide arrays vs. metaphase-comparative genomic hybridisation and BAC arrays for single-cell analysis: first applications to preimplantation genetic diagnosis for Robertsonian translocation carriers.

    Laia Ramos

    Full Text Available Comprehensive chromosome analysis techniques such as metaphase-Comparative Genomic Hybridisation (CGH and array-CGH are available for single-cell analysis. However, while metaphase-CGH and BAC array-CGH have been widely used for Preimplantation Genetic Diagnosis, oligonucleotide array-CGH has not been used in an extensive way. A comparison between oligonucleotide array-CGH and metaphase-CGH has been performed analysing 15 single fibroblasts from aneuploid cell-lines and 18 single blastomeres from human cleavage-stage embryos. Afterwards, oligonucleotide array-CGH and BAC array-CGH were also compared analysing 16 single blastomeres from human cleavage-stage embryos. All three comprehensive analysis techniques provided broadly similar cytogenetic profiles; however, non-identical profiles appeared when extensive aneuploidies were present in a cell. Both array techniques provided an optimised analysis procedure and a higher resolution than metaphase-CGH. Moreover, oligonucleotide array-CGH was able to define extra segmental imbalances in 14.7% of the blastomeres and it better determined the specific unbalanced chromosome regions due to a higher resolution of the technique (≈ 20 kb. Applicability of oligonucleotide array-CGH for Preimplantation Genetic Diagnosis has been demonstrated in two cases of Robertsonian translocation carriers 45,XY,der(13;14(q10;q10. Transfer of euploid embryos was performed in both cases and pregnancy was achieved by one of the couples. This is the first time that an oligonucleotide array-CGH approach has been successfully applied to Preimplantation Genetic Diagnosis for balanced chromosome rearrangement carriers.

  18. Oligonucleotide Arrays vs. Metaphase-Comparative Genomic Hybridisation and BAC Arrays for Single-Cell Analysis: First Applications to Preimplantation Genetic Diagnosis for Robertsonian Translocation Carriers

    Ramos, Laia; del Rey, Javier; Daina, Gemma; García-Aragonés, Manel; Armengol, Lluís; Fernandez-Encinas, Alba; Parriego, Mònica; Boada, Montserrat; Martinez-Passarell, Olga; Martorell, Maria Rosa; Casagran, Oriol; Benet, Jordi; Navarro, Joaquima

    2014-01-01

    Comprehensive chromosome analysis techniques such as metaphase-Comparative Genomic Hybridisation (CGH) and array-CGH are available for single-cell analysis. However, while metaphase-CGH and BAC array-CGH have been widely used for Preimplantation Genetic Diagnosis, oligonucleotide array-CGH has not been used in an extensive way. A comparison between oligonucleotide array-CGH and metaphase-CGH has been performed analysing 15 single fibroblasts from aneuploid cell-lines and 18 single blastomeres from human cleavage-stage embryos. Afterwards, oligonucleotide array-CGH and BAC array-CGH were also compared analysing 16 single blastomeres from human cleavage-stage embryos. All three comprehensive analysis techniques provided broadly similar cytogenetic profiles; however, non-identical profiles appeared when extensive aneuploidies were present in a cell. Both array techniques provided an optimised analysis procedure and a higher resolution than metaphase-CGH. Moreover, oligonucleotide array-CGH was able to define extra segmental imbalances in 14.7% of the blastomeres and it better determined the specific unbalanced chromosome regions due to a higher resolution of the technique (≈20 kb). Applicability of oligonucleotide array-CGH for Preimplantation Genetic Diagnosis has been demonstrated in two cases of Robertsonian translocation carriers 45,XY,der(13;14)(q10;q10). Transfer of euploid embryos was performed in both cases and pregnancy was achieved by one of the couples. This is the first time that an oligonucleotide array-CGH approach has been successfully applied to Preimplantation Genetic Diagnosis for balanced chromosome rearrangement carriers. PMID:25415307

  19. CGH Supports World Cancer Day Every Day

    We celebrate World Cancer Day every year on February 4th. This year the theme “We can. I can.” invites us to think not only about how we can work with one another to reduce the global burden of cancer, but how we as individuals can make a difference. Every day the staff at CGH work to establish and build upon programs that are aimed at improving the lives of people affected by cancer.

  20. Analysis of aCGH Integrating Different Sources of Information by Means of a CBR

    Zato Domínguez, Carolina; de Paz Santana, Juan F.; Bajo Pérez, Javier; Corchado Rodríguez, Juan M.

    2011-01-01

    Knowledge management is a key element in medical environments. Arrays CGH make possible the realization of tests on patients for the detection of mutations in chromosomal regions. The detection of the regions with mutations associated to different pathologies is an important step for the selection of relevant genes, proteins or diseases. The corresponding information of the mutations and genes is distributed in dif- ferent public sources and databases, so it is necessary the use of systems th...

  1. Introduction to embedded system design using field programmable gate arrays

    Dubey, Rahul

    2009-01-01

    Offers information on the use of field programmable gate arrays (FPGAs) in the design of embedded systems. This text considers a hypothetical robot controller as an embedded application and weaves around it related concepts of FPGA-based digital design. It is suitable for both students and designers who have worked with microprocessors.

  2. Heat-rejection design for large concentrating solar arrays

    French, E. P.

    1980-01-01

    This paper considers the effect of heat rejection devices (radiators) on the performance and cost of large concentrating solar arrays for space application. Overall array characteristics are derived from the weight, cost, and performance of four major components; namely primary structure, optics/secondary structure, radiator, and solar panel. An ideal concentrator analysis is used to establish general cost and performance trends independent of specific array design. Both passive and heat-pipe radiation are evaluated, with an incremental cost-of-power approach used in the evaluation. Passive radiators are found to be more cost effective with silicon than with gallium arsenide (GaAs) arrays. Representative concentrating arrays have been evaluated for both near-term and advanced solar cell technology. Minimum cost of power is achieved at geometric concentration ratios in the range 2 to 6.

  3. Phased-array radar design application of radar fundamentals

    Jeffrey, Thomas

    2009-01-01

    Phased-Array Radar Design is a text-reference designed for electrical engineering graduate students in colleges and universities as well as for corporate in-house training programs for radar design engineers, especially systems engineers and analysts who would like to gain hands-on, practical knowledge and skills in radar design fundamentals, advanced radar concepts, trade-offs for radar design and radar performance analysis.

  4. A study into the design of steerable microphone arrays

    Lai, Chiong Ching; Leung, Yee Hong

    2017-01-01

    The book covers the design formulations for broadband beamformer targeting nearfield and farfield sources. The book content includes background information on the acoustic environment, including propagation medium, the array geometries, signal models and basic beamformer designs. Subsequently it introduces design formulation for nearfield, farfield and mixed nearfield-farfield beamformers and extends the design formulation into electronically steerable beamformers. In addition, a robust formulation is introduced for all the designs mentioned.

  5. Design and control of phased ICRF antenna arrays

    Goulding, R.H.; Baity, F.W.; Hoffman, D.J.

    1993-01-01

    Phased antenna arrays operating in the ion cyclotron range of frequencies (ICRF) are used to produce highly directional wave spectra, primarily for use in current drive experiments. RF current drive using phased antennas has been demonstrated in both the JET and DIII-D tokamaks, and both devices are planning to operate new four-element arrays beginning early next year. Features of antenna design that are relevant to phased operation and production of directional spectra are reviewed. Recent advances in the design of the feed circuits and the related control systems for these arrays should substantially improve their performance, by reducing the coupling seen by the matching networks and rf power supplies caused by the mutual impedance of the array elements. The feed circuit designs for the DIII-D and JET phased antenna arrays are compared. The two configurations differ significantly due to the fact that one power amplifier is used for the entire array in the former case, and one per element in the latter. The JET system uses automatic feedback control of matching, phase and amplitude of antenna currents, and the transmitter power balance. The design of this system is discussed, and a time dependent model used to predict its behavior is described

  6. Coil Array Design Inspired on the Kepler's Lenten Pretzel

    Vazquez, F.; Solis, S. E.; Rodriguez, A. O.

    2008-01-01

    The RF coil arrays are an important part in Magnetic Resonance Imaging, since they are the main device for transmission and reception of the magnetic resonance signal. An RF coil array with a new configuration based on the Kepler's Lenten pretzel for the geocentric path of Mars is proposed in this work. The evenly distributed trajectories may serve as the basic configuration to form a coil array to adequately cover a region of interest for magnetic resonance experiments. The main goal is to investigate the electromagnetic properties of this coil array geometry to obtain an optimal design for its further construction. Hence, the electromagnetic properties of the coil array were numerical simulated using the finite element method and the quasi-static approach. Resulting simulations showed that there is an important concentration of magnetic field lines at the centre of the coil array. This is an advantage over other coil arrays where the magnetic field usually decreased at their geometrical centre. Both the electric and magnetic fields had also a very good uniformity. These characteristics made this coil design a good candidate for applications where the use of multi-coil technology is mandatory

  7. Array design and expression evaluation in POOMA II

    Karmesin, S.; Crotinger, J.; Cummings, J.; Haney, S.; Humphrey, W.; Reynders, J.; Smith, S.; Williams, T.J.

    1998-12-31

    POOMA is a templated C++ class library for use in the development of large-scale scientific simulations on serial and parallel computers. POOMA II is a new design and implementation of POOMA intended to add richer capabilities and greater flexibility to the framework. The new design employs a generic Array class that acts as an interface to, or view on, a wide variety of data representation objects referred to as engines. This design separates the interface and the representation of multidimensional arrays. The separation is achieved using compile-time techniques rather than virtual functions, and thus code efficiency is maintained. POOMA II uses PETE, the Portable Expression Template Engine, to efficiently represent complex mathematical expressions involving arrays and other objects. The representation of expressions is kept separate from expression evaluation, allowing the use of multiple evaluator mechanisms that can support nested where-block constructs, hardware-specific optimizations and different run-time environments.

  8. Design of nested Halbach cylinder arrays for magnetic refrigeration applications

    Trevizoli, Paulo V., E-mail: trevizoli@polo.ufsc.br; Lozano, Jaime A.; Peixer, Guilherme F.; Barbosa Jr, Jader R.

    2015-12-01

    We present an experimentally validated analytical procedure to design nested Halbach cylinder arrays for magnetic cooling applications. The procedure aims at maximizing the magnetic flux density variation in the core of the array for a given set of design parameters, namely the inner diameter of the internal magnet, the air gap between the magnet cylinders, the number of segments of each magnet and the remanent flux density of the Nd{sub 2}Fe{sub 14}B magnet grade. The design procedure was assisted and verified by 3-D numerical modeling using a commercial software package. An important aspect of the optimal design is to maintain an uniform axial distribution of the magnetic flux density in the region of the inner gap occupied by the active magnetocaloric regenerator. An optimal nested Halbach cylinder array was manufactured and experimentally evaluated for the magnetic flux density in the inner gap. The analytically calculated magnetic flux density variation agreed to within 5.6% with the experimental value for the center point of the magnet gap. - Highlights: • An analytical procedure to design nested Halbach cylinder arrays is proposed. • An optimal magnet configuration was built based on the analytical procedure. • The procedure was validated with 3D COMSOL simulations and experimental data.

  9. Design considerations for large roof-integrated photovoltaic arrays

    Ropp, M.E.; Begovic, M.; Rohatgi, A. [Georgia Inst. of Tech., Atlanta, GA (United States); Long, R. [Georgia Institute of Technology, Atlanta (United States). Office of Facilities

    1997-01-01

    This paper describes calculations and modeling used in the design of the photovoltaic (PV) array built on the roof of the Georgia Tech Aquatic Center, the aquatic sports venue for the 1996 Olympic and Paralympic Games. The software package PVFORM (version 3.3) was extensively utilized; because of its importance to this work, it is thoroughly reviewed here. Procedures required to adapt PVFORM to this particular installation are described. The expected behavior and performance of the system, including maximum power output, annual energy output and maximum expected temperature, are then presented, and the use of this information in making informed design decisions is described. Finally, since the orientation of the PV array is not optimal, the effect of the unoptimized array orientation on the system`s performance is quantified. (author)

  10. Optical alignment using a CGH and an autostigmatic microscope

    Parks, Robert E.

    2017-08-01

    We show how custom computer generated holograms (CGH) are used along with an autostigmatic microscope (ASM) to align both optical and mechanical components relative to the CGH. The patterns in the CGHs define points and lines in space when interrogated with the focus of the ASM. Once the ASM is aligned to the CGH, an optical or mechanical component such as a lens, a well-polished ball or a cylinder can be aligned to the ASM in 3 or 4 degrees of freedom and thus to the CGH. In this case we show how a CGH is used to make a fixture for cementing a doublet lens without the need for a rotary table or a precision vertical stage.

  11. Laser beam shaping design based on micromirror array

    Fang, Han; Su, Bida; Liu, Jiaguo; Fan, Xiaoli; Jing, Wang

    2017-10-01

    In the practical application of the laser, it is necessary to use the laser beam shaping technology to shape the output beam of laser device to the uniform light intensity distribution. The shaping divergent optical system of compound eye integrator way is composed of beam expanding mirror group and lens array. Its working principle is to expand the output laser to a certain size of caliber, and then divide the beam with lens array into multiple sub beam, where the lens unit of lens array can control the divergence angle of sub beam through the design of focal length, with mutual superposition of the sub beam in far field, to make up for the nonuniformity of beam, so that the radiant exitance on the radiated surface may become uniform. In this paper, we use a reflective microlens array to realize the laser beam shaping. By through of the practical optical path model established, the ray tracing is carried out and the simulation results for single-mode Gaussian beam with noise circumstance is provided. The analysis results show that the laser beam shaping under different inputs can be effectively realized by use of microlens array. All the energy is within the signal window, with a high energy efficiency of more than 90%; The measured surface has a better uniformity, and the uniformity is better than 99.5% at 150m.

  12. Electromagnetic linear machines with dual Halbach array design and analysis

    Yan, Liang; Peng, Juanjuan; Zhang, Lei; Jiao, Zongxia

    2017-01-01

    This book extends the conventional two-dimensional (2D) magnet arrangement into 3D pattern for permanent magnet linear machines for the first time, and proposes a novel dual Halbach array. It can not only effectively increase the radial component of magnetic flux density and output force of tubular linear machines, but also significantly reduce the axial flux density, radial force and thus system vibrations and noises. The book is also the first to address the fundamentals and provide a summary of conventional arrays, as well as novel concepts for PM pole design in electric linear machines. It covers theoretical study, numerical simulation, design optimization and experimental works systematically. The design concept and analytical approaches can be implemented to other linear and rotary machines with similar structures. The book will be of interest to academics, researchers, R&D engineers and graduate students in electronic engineering and mechanical engineering who wish to learn the core principles, met...

  13. Array-CGH detection of three cryptic submicroscopic imbalances in ...

    2The Second Clinical Medical College (Shenzhen People's Hospital), Jinan University, Guangdong Province. 518020, People's Republic of China ... Here, we describe a phenotypically normal prenatal case who was found to carry an apparently balanced .... Precise definitions of CCRs and their real complexity can only be ...

  14. Design of photovoltaic central power station concentrator array

    1984-02-01

    A design for a photovoltaic central power station using tracking concentrators has been developed. The 100 MW plant is assumed to be located adjacent to the Saguaro Power Station of Arizona Public Service. The design assumes an advanced Martin Marietta two-axis tracking fresnel lens concentrator. The concentrators are arrayed in 5 MW subfields, each with its own power conditioning unit. The photovoltaic plant output is connected to the existing 115 kV switchyard. The site specific design allows detailed cost estimates for engineering, site preparation, and installation. Collector and power conditioning costs have been treated parametrically.

  15. High Performance Systolic Array Core Architecture Design for DNA Sequencer

    Saiful Nurdin Dayana

    2018-01-01

    Full Text Available This paper presents a high performance systolic array (SA core architecture design for Deoxyribonucleic Acid (DNA sequencer. The core implements the affine gap penalty score Smith-Waterman (SW algorithm. This time-consuming local alignment algorithm guarantees optimal alignment between DNA sequences, but it requires quadratic computation time when performed on standard desktop computers. The use of linear SA decreases the time complexity from quadratic to linear. In addition, with the exponential growth of DNA databases, the SA architecture is used to overcome the timing issue. In this work, the SW algorithm has been captured using Verilog Hardware Description Language (HDL and simulated using Xilinx ISIM simulator. The proposed design has been implemented in Xilinx Virtex -6 Field Programmable Gate Array (FPGA and improved in the core area by 90% reduction.

  16. Design and Analysis of MEMS Linear Phased Array

    Guoxiang Fan

    2016-01-01

    Full Text Available A structure of micro-electro-mechanical system (MEMS linear phased array based on “multi-cell” element is designed to increase radiation sound pressure of transducer working in bending vibration mode at high frequency. In order to more accurately predict the resonant frequency of an element, the theoretical analysis of the dynamic equation of a fixed rectangular composite plate and finite element method simulation are adopted. The effects of the parameters both in the lateral and elevation direction on the three-dimensional beam directivity characteristics are comprehensively analyzed. The key parameters in the analysis include the “cell” number of element, “cell” size, “inter-cell” spacing and the number of elements, element width. The simulation results show that optimizing the linear array parameters both in the lateral and elevation direction can greatly improve the three-dimensional beam focusing for MEMS linear phased array, which is obviously different from the traditional linear array.

  17. A CMOS ASIC Design for SiPM Arrays.

    Dey, Samrat; Banks, Lushon; Chen, Shaw-Pin; Xu, Wenbin; Lewellen, Thomas K; Miyaoka, Robert S; Rudell, Jacques C

    2011-12-01

    Our lab has previously reported on novel board-level readout electronics for an 8×8 silicon photomultiplier (SiPM) array featuring row/column summation technique to reduce the hardware requirements for signal processing. We are taking the next step by implementing a monolithic CMOS chip which is based on the row-column architecture. In addition, this paper explores the option of using diagonal summation as well as calibration to compensate for temperature and process variations. Further description of a timing pickoff signal which aligns all of the positioning (spatial channels) pulses in the array is described. The ASIC design is targeted to be scalable with the detector size and flexible to accommodate detectors from different vendors. This paper focuses on circuit implementation issues associated with the design of the ASIC to interface our Phase II MiCES FPGA board with a SiPM array. Moreover, a discussion is provided for strategies to eventually integrate all the analog and mixed-signal electronics with the SiPM, on either a single-silicon substrate or multi-chip module (MCM).

  18. 1024x1024 resistive emitter array design and fabrication status

    Bryant, Paul T.; Oleson, Jim; McHugh, Stephen W.; Beuville, Eric; Schlesselmann, John D.; Woolaway, James T.; Barskey, Steve; Solomon, Steven L.; Joyner, Thomas W.

    2002-07-01

    Santa Barbara Infrared (SBIR) is producing a high performance 1,024 x 1,024 Large Format Resistive emitter Array (LFRA) for use in the next generation of IR Scene Projectors (IRSPs). LFRA requirements were developed through close cooperation with the Tri-Service IR Scene Projector working group, and through detailed trade studies sponsored by the OSD Central T&E Investment Program (CTEIP) and a Phase I US Navy Small Business Innovative Research (SBIR) contract. The CMOS Read-In Integrated Circuit (RIIC) is being designed by SBIR and Indigo Systems under a Small Business Innovative Research (SBIR) contract. Performance and features include 750 K MWIR maximum apparent temperature, 5 ms radiance rise time, 200 Hz full frame update, and 400 Hz window mode operation. Ten 8-inch CMOS wafers will be fabricated and characterized in mid-2002, followed by emitter fabrication in late 2002. This paper discusses array performance, requirements flow-down, array design, fabrication of 2 X 2-inch CMOS devices, and plans for subsequent RIIC wafer test and emitter pixel fabrication.

  19. Solar cell array design handbook - The principles and technology of photovoltaic energy conversion

    Rauschenbach, H. S.

    1980-01-01

    Photovoltaic solar cell array design and technology for ground-based and space applications are discussed from the user's point of view. Solar array systems are described, with attention given to array concepts, historical development, applications and performance, and the analysis of array characteristics, circuits, components, performance and reliability is examined. Aspects of solar cell array design considered include the design process, photovoltaic system and detailed array design, and the design of array thermal, radiation shielding and electromagnetic components. Attention is then given to the characteristics and design of the separate components of solar arrays, including the solar cells, optical elements and mechanical elements, and the fabrication, testing, environmental conditions and effects and material properties of arrays and their components are discussed.

  20. Nanoparticle array based optical frequency selective surfaces: theory and design.

    Saeidi, Chiya; van der Weide, Daniel

    2013-07-01

    We demonstrate a synthesis procedure for designing a bandstop optical frequency selective surface (FSS) composed of nanoparticle (NP) elements. The proposed FSS uses two-dimensional (2-D) periodic arrays of NPs with subwavelength unit-cell dimensions. We derive equivalent circuit for a nanoparticle array (NPA) using the closed-form solution for a 2-D NPA excited by a plane wave in the limit of the dipole approximation, which includes contribution from both individual and collective plasmon modes. Using the extracted equivalent circuit, we demonstrate synthesis of an optical FSS using cascaded NPA layers as coupled resonators, which we validate with both circuit model and full-wave simulation for a third-order Butterworth bandstop prototype.

  1. Reconfigurable signal processor designs for advanced digital array radar systems

    Suarez, Hernan; Zhang, Yan (Rockee); Yu, Xining

    2017-05-01

    The new challenges originated from Digital Array Radar (DAR) demands a new generation of reconfigurable backend processor in the system. The new FPGA devices can support much higher speed, more bandwidth and processing capabilities for the need of digital Line Replaceable Unit (LRU). This study focuses on using the latest Altera and Xilinx devices in an adaptive beamforming processor. The field reprogrammable RF devices from Analog Devices are used as analog front end transceivers. Different from other existing Software-Defined Radio transceivers on the market, this processor is designed for distributed adaptive beamforming in a networked environment. The following aspects of the novel radar processor will be presented: (1) A new system-on-chip architecture based on Altera's devices and adaptive processing module, especially for the adaptive beamforming and pulse compression, will be introduced, (2) Successful implementation of generation 2 serial RapidIO data links on FPGA, which supports VITA-49 radio packet format for large distributed DAR processing. (3) Demonstration of the feasibility and capabilities of the processor in a Micro-TCA based, SRIO switching backplane to support multichannel beamforming in real-time. (4) Application of this processor in ongoing radar system development projects, including OU's dual-polarized digital array radar, the planned new cylindrical array radars, and future airborne radars.

  2. Thermal design of spacecraft solar arrays using a polyimide foam

    Bianco, N; Iasiello, M; Naso, V

    2015-01-01

    The design of the Thermal Control System (TCS) of spacecraft solar arrays plays a fundamental role. Indeed, the spacecraft components must operate within a certain range of temperature. If this doesn't occur, their performance is reduced and they may even break. Solar arrays, which are employed to recharge batteries, are directly exposed to the solar heat flux, and they need to be insulated from the earth's surface irradiation. Insulation is currently provided either with a white paint coating or with a Multi Layer Insulation (MLI) system [1]. A configuration based on an open-cell polyimide foam has also been recently proposed [2]. Using polyimide foams in TCSs looks very attractive in terms of costs, weight and assembling. An innovative thermal analysis of the above cited TCS configurations is carried out in this paper, by solving the porous media energy equation, under the assumption of Local Thermal Equilibrium (LTE) between the two phases. Radiation effects through the solar array are also considered by using the Rosseland approximation. Under a stationary daylight condition, temperature profiles are obtained by means of the finite-element based code COMSOL Multiphysics ® . Finally, since the weight plays an important role in aerospace applications, weights of the three TCS configurations are compared. (paper)

  3. Thermal design of spacecraft solar arrays using a polyimide foam

    Bianco, N.; Iasiello, M.; Naso, V.

    2015-11-01

    The design of the Thermal Control System (TCS) of spacecraft solar arrays plays a fundamental role. Indeed, the spacecraft components must operate within a certain range of temperature. If this doesn't occur, their performance is reduced and they may even break. Solar arrays, which are employed to recharge batteries, are directly exposed to the solar heat flux, and they need to be insulated from the earth's surface irradiation. Insulation is currently provided either with a white paint coating or with a Multi Layer Insulation (MLI) system [1]. A configuration based on an open-cell polyimide foam has also been recently proposed [2]. Using polyimide foams in TCSs looks very attractive in terms of costs, weight and assembling. An innovative thermal analysis of the above cited TCS configurations is carried out in this paper, by solving the porous media energy equation, under the assumption of Local Thermal Equilibrium (LTE) between the two phases. Radiation effects through the solar array are also considered by using the Rosseland approximation. Under a stationary daylight condition, temperature profiles are obtained by means of the finite-element based code COMSOL Multiphysics®. Finally, since the weight plays an important role in aerospace applications, weights of the three TCS configurations are compared.

  4. Low Complexity Beampattern Design in MIMO Radars Using Planar Array

    Bouchoucha, Taha

    2015-01-07

    In multiple-input multiple-output radar systems, it is usually desirable to steer transmitted power in the region-of-interest. To do this, conventional methods optimize the waveform covariance matrix, R, for the desired beampattern, which is then used to generate actual transmitted waveforms. Both steps require constrained optimization, therefore, use iterative and expensive algorithms. In this paper, we provide a closed-form solution to design covariance matrix for the given beampattern using the planar array, which is then used to derive a novel closed-form algorithm to directly design the finite-alphabet constant-envelope (FACE) waveforms. The proposed algorithm exploits the two-dimensional fast-Fourier-transform. The performance of our proposed algorithm is compared with existing methods that are based on semi-definite quadratic programming with the advantage of a considerably reduced complexity.

  5. Improved analysis of bacterial CGH data beyond the log-ratio paradigm

    Aakra Ågot

    2009-03-01

    Full Text Available Abstract Background Existing methods for analyzing bacterial CGH data from two-color arrays are based on log-ratios only, a paradigm inherited from expression studies. We propose an alternative approach, where microarray signals are used in a different way and sequence identity is predicted using a supervised learning approach. Results A data set containing 32 hybridizations of sequenced versus sequenced genomes have been used to test and compare methods. A ROC-analysis has been performed to illustrate the ability to rank probes with respect to Present/Absent calls. Classification into Present and Absent is compared with that of a gaussian mixture model. Conclusion The results indicate our proposed method is an improvement of existing methods with respect to ranking and classification of probes, especially for multi-genome arrays.

  6. Analysis of neighborhood behavior in lead optimization and array design.

    Papadatos, George; Cooper, Anthony W J; Kadirkamanathan, Visakan; Macdonald, Simon J F; McLay, Iain M; Pickett, Stephen D; Pritchard, John M; Willett, Peter; Gillet, Valerie J

    2009-02-01

    Neighborhood behavior describes the extent to which small structural changes defined by a molecular descriptor are likely to lead to small property changes. This study evaluates two methods for the quantification of neighborhood behavior: the optimal diagonal method of Patterson et al. and the optimality criterion method of Horvath and Jeandenans. The methods are evaluated using twelve different types of fingerprint (both 2D and 3D) with screening data derived from several lead optimization projects at GlaxoSmithKline. The principal focus of the work is the design of chemical arrays during lead optimization, and the study hence considers not only biological activity but also important drug properties such as metabolic stability, permeability, and lipophilicity. Evidence is provided to suggest that the optimality criterion method may provide a better quantitative description of neighborhood behavior than the optimal diagonal method.

  7. CGH Celebrates Take Your Child To Work Day 2015

    Shady Grove celebrated Take Your Child To Work Day this year with a variety of activities and sessions aimed at inspiring school-aged children to explore career paths in science and public service. CGH hosted its inaugural Take Your Child To Work Day session: An Introduction to Global Health.

  8. Design of Circularly-Polarised, Crossed Drooping Dipole, Phased Array Antenna Using Genetic Algorithm Optimisation

    Larsen, Niels Vesterdal

    2007-01-01

    A printed drooping dipole array is designed and constructed. The design is based on a genetic algorithm optimisation procedure used in conjunction with the software programme AWAS. By optimising the array G/T for specific combinations of scan angles and frequencies an optimum design is obtained...

  9. Massively parallel sequencing, aCGH, and RNA-Seq technologies provide a comprehensive molecular diagnosis of Fanconi anemia.

    Chandrasekharappa, Settara C; Lach, Francis P; Kimble, Danielle C; Kamat, Aparna; Teer, Jamie K; Donovan, Frank X; Flynn, Elizabeth; Sen, Shurjo K; Thongthip, Supawat; Sanborn, Erica; Smogorzewska, Agata; Auerbach, Arleen D; Ostrander, Elaine A

    2013-05-30

    Current methods for detecting mutations in Fanconi anemia (FA)-suspected patients are inefficient and often miss mutations. We have applied recent advances in DNA sequencing and genomic capture to the diagnosis of FA. Specifically, we used custom molecular inversion probes or TruSeq-enrichment oligos to capture and sequence FA and related genes, including introns, from 27 samples from the International Fanconi Anemia Registry at The Rockefeller University. DNA sequencing was complemented with custom array comparative genomic hybridization (aCGH) and RNA sequencing (RNA-seq) analysis. aCGH identified deletions/duplications in 4 different FA genes. RNA-seq analysis revealed lack of allele specific expression associated with a deletion and splicing defects caused by missense, synonymous, and deep-in-intron variants. The combination of TruSeq-targeted capture, aCGH, and RNA-seq enabled us to identify the complementation group and biallelic germline mutations in all 27 families: FANCA (7), FANCB (3), FANCC (3), FANCD1 (1), FANCD2 (3), FANCF (2), FANCG (2), FANCI (1), FANCJ (2), and FANCL (3). FANCC mutations are often the cause of FA in patients of Ashkenazi Jewish (AJ) ancestry, and we identified 2 novel FANCC mutations in 2 patients of AJ ancestry. We describe here a strategy for efficient molecular diagnosis of FA.

  10. Cross-platform array comparative genomic hybridization meta-analysis separates hematopoietic and mesenchymal from epithelial tumors

    Jong, C.; Marchiori, E.; van der Vaart, A.W.; Chin, S.F.; Carvalho, B; Tijssen, M.; Eijk, P.P.; van den IJssel, P.; Grabsch, H.; Quirke, P.; Oudejans, J.J.; Meijer, G.J.; Caldas, C.; Ylstra, B.

    2007-01-01

    A series of studies have been published that evaluate the chromosomal copy number changes of different tumor classes using array comparative genomic hybridization (array CGH); however, the chromosomal aberrations that distinguish the different tumor classes have not been fully characterized.

  11. Algorithms and Array Design Criteria for Robust Imaging in Interferometry

    Kurien, Binoy George

    Optical interferometry is a technique for obtaining high-resolution imagery of a distant target by interfering light from multiple telescopes. Image restoration from interferometric measurements poses a unique set of challenges. The first challenge is that the measurement set provides only a sparse-sampling of the object's Fourier Transform and hence image formation from these measurements is an inherently ill-posed inverse problem. Secondly, atmospheric turbulence causes severe distortion of the phase of the Fourier samples. We develop array design conditions for unique Fourier phase recovery, as well as a comprehensive algorithmic framework based on the notion of redundant-spaced-calibration (RSC), which together achieve reliable image reconstruction in spite of these challenges. Within this framework, we see that classical interferometric observables such as the bispectrum and closure phase can limit sensitivity, and that generalized notions of these observables can improve both theoretical and empirical performance. Our framework leverages techniques from lattice theory to resolve integer phase ambiguities in the interferometric phase measurements, and from graph theory, to select a reliable set of generalized observables. We analyze the expected shot-noise-limited performance of our algorithm for both pairwise and Fizeau interferometric architectures and corroborate this analysis with simulation results. We apply techniques from the field of compressed sensing to perform image reconstruction from the estimates of the object's Fourier coefficients. The end result is a comprehensive strategy to achieve well-posed and easily-predictable reconstruction performance in optical interferometry.

  12. Asterias: A Parallelized Web-based Suite for the Analysis of Expression and aCGH Data

    Ramón Díaz-Uriarte

    2007-01-01

    Full Text Available The analysis of expression and CGH arrays plays a central role in the study of complex diseases, especially cancer, including finding markers for early diagnosis and prognosis, choosing an optimal therapy, or increasing our understanding of cancer development and metastasis. Asterias (http://www.asterias.info is an integrated collection of freely-accessible web tools for the analysis of gene expression and aCGH data. Most of the tools use parallel computing (via MPI and run on a server with 60 CPUs for computation; compared to a desktop or server-based but not parallelized application, parallelization provides speed ups of factors up to 50. Most of our applications allow the user to obtain additional information for user-selected genes (chromosomal location, PubMed ids, Gene Ontology terms, etc. by using clickable links in tables and/or fi gures. Our tools include: normalization of expression and aCGH data (DNMAD; converting between different types of gene/clone and protein identifi ers (IDconverter/IDClight; fi ltering and imputation (preP; finding differentially expressed genes related to patient class and survival data (Pomelo II; searching for models of class prediction (Tnasas; using random forests to search for minimal models for class prediction or for large subsets of genes with predictive capacity (GeneSrF; searching for molecular signatures and predictive genes with survival data (SignS; detecting regions of genomic DNA gain or loss (ADaCGH. The capability to send results between different applications, access to additional functional information, and parallelized computation make our suite unique and exploit features only available to web-based applications.

  13. Low-cost modular array-field designs for flat-panel and concentrator photovoltaic systems

    Post, H. N.; Carmichael, D. C.; Alexander, G.; Castle, J. A.

    1982-09-01

    Described are the design and development of low-cost, modular array fields for flat-panel and concentrator photovoltaic (PV) systems. The objective of the work was to reduce substantially the cost of the array-field Balance-of-System (BOS) subsystems and site-specific design costs as compared to previous PV installations. These subsystems include site preparation, foundations, support structures, electrical writing, grounding, lightning protection, electromagnetic interference considerations, and controls. To reduce these BOS and design costs, standardized modular (building-block) designs for flat-panel and concentrator array fields have been developed that are fully integrated and optimized for lowest life-cycle costs. Using drawings and specifications now available, these building-block designs can be used in multiples to install various size array fields. The developed designs are immediately applicable (1982) and reduce the array-field BOS costs to a fraction of previous costs.

  14. Improved Design of Microphone-Array Hearing Aids

    Greenberg, Julie

    1994-01-01

    ...). Research on microphone array hearing aids is motivated by the lack of success of single-microphone systems, as well as the documented advantages of binaural hearing and multiple-element sensing systems...

  15. Quasi-optical antenna-mixer-array design for terahertz frequencies

    Guo, Yong; Potter, Kent A.; Rutledge, David B.

    1992-01-01

    A new quasi-optical antenna-mixer-array design for terahertz frequencies is presented. In the design, antenna and mixer are combined into an entity, based on the technology in which millimeter-wave horn antenna arrays have been fabricated in silicon wafers. It consists of a set of forward- and backward-looking horns made with a set of silicon wafers. The front side is used to receive incoming signal, and the back side is used to feed local oscillator signal. Intermediate frequency is led out from the side of the array. Signal received by the horn array is picked up by antenna probes suspended on thin silicon-oxynitride membranes inside the horns. Mixer diodes will be located on the membranes inside the horns. Modeling of such an antenna-mixer-array design is done on a scaled model at microwave frequencies. The impedance matching, RF and LO isolation, and patterns of the array have been tested and analyzed.

  16. Analysis of human HPRT- deletion mutants by the microarray-CGH (comparative genomic hybridization)

    Kodaira, M.; Sasaki, K.; Tagawa, H.; Omine, H.; Kushiro, J.; Takahashi, N.; Katayama, H.

    2003-01-01

    We are trying to evaluate genetic effects of radiation on human using mutation frequency as an indicator. For the efficient detection of mutations, it is important to understand the mechanism and the characteristics of radiation-induced mutations. We have started the analysis of hypoxanthine-guanine phosphoribosyl transferase (HPRT) mutants induced by X-ray in order to clarify the deletion size and the mutation-distribution. We analyzed 39 human X-ray induced HPRT-deletion mutants by using the microarray-CGH. The array for this analysis contains 57 BAC clones covering as much as possible of the 4Mb of the 5' side and 10Mb of the 3' side of the HPRT gene based on the NCBI genome database. DNA from parent strain and each HPRT-mutant strain are labeled with Cy5 and Cy3 respectively, and were mixed and hybridized on the array. Fluorescent intensity ratio of the obtained spots was analyzed using software we developed to identify clones corresponding to the deletion region. The deletion in these strains ranged up to 3.5 Mb on the 5' side and 6 Mb on the 3' side of the HPRT gene. Deletions in 13 strains ended around BAC clones located at about 3 Mb on the 5' side. On the 3' side, deletions extended up to the specific clones located at 1.5 Mb in 11 strains. The mutations seem to be complex on the 3' end of deletion; some accompanied duplications with deletions and others could not be explained by one mutation event. We need to confirm these results, taking into account the experimental reproducibility and the accuracy of the published genetic map. The results of the research using the microarray-CGH help us to search the regions where deletions are easily induced and to identify the factors affecting the range of deletions

  17. Physical analysis for designing nested-wire arrays on Z-pinch implosion

    Yang Zhenhua; Liu Quan; Ding Ning; Ning Cheng

    2005-01-01

    Z-pinch experiments have demonstrated that the X-ray power increases 40% with a nested-wire array compared with that with a single-layered wire array. The design of the nested-wire array on Z accelerator is studied through the implosion dynamics and the growth of RT instabilities. The analysis shows that the nested-wire array does not produce more total X-ray radiation energy than the single-layered wire array, but it obviously increases the X-ray power. The radius of the outer array of the nested-wire array could be determined based on the radius of the optimized single-layered. The masses of the outer and inner arrays could be determined by the implosion time of the nested-wire array, which is roughly the same as that of the single-layered wire array. Some suggestions are put forward which may be helpful in the nested-wire array design for Z-pinch experiments. (authors)

  18. A web server for mining Comparative Genomic Hybridization (CGH) data

    Liu, Jun; Ranka, Sanjay; Kahveci, Tamer

    2007-11-01

    Advances in cytogenetics and molecular biology has established that chromosomal alterations are critical in the pathogenesis of human cancer. Recurrent chromosomal alterations provide cytological and molecular markers for the diagnosis and prognosis of disease. They also facilitate the identification of genes that are important in carcinogenesis, which in the future may help in the development of targeted therapy. A large amount of publicly available cancer genetic data is now available and it is growing. There is a need for public domain tools that allow users to analyze their data and visualize the results. This chapter describes a web based software tool that will allow researchers to analyze and visualize Comparative Genomic Hybridization (CGH) datasets. It employs novel data mining methodologies for clustering and classification of CGH datasets as well as algorithms for identifying important markers (small set of genomic intervals with aberrations) that are potentially cancer signatures. The developed software will help in understanding the relationships between genomic aberrations and cancer types.

  19. Computer-aided engineering system for design of sequence arrays and lithographic masks

    Hubbell, Earl A.; Morris, MacDonald S.; Winkler, James L.

    1996-01-01

    An improved set of computer tools for forming arrays. According to one aspect of the invention, a computer system (100) is used to select probes and design the layout of an array of DNA or other polymers with certain beneficial characteristics. According to another aspect of the invention, a computer system uses chip design files (104) to design and/or generate lithographic masks (110).

  20. Design structure for in-system redundant array repair in integrated circuits

    Bright, Arthur A.; Crumley, Paul G.; Dombrowa, Marc; Douskey, Steven M.; Haring, Rudolf A.; Oakland, Steven F.; Quellette, Michael R.; Strissel, Scott A.

    2008-11-25

    A design structure for repairing an integrated circuit during operation of the integrated circuit. The integrated circuit comprising of a multitude of memory arrays and a fuse box holding control data for controlling redundancy logic of the arrays. The design structure provides the integrated circuit with a control data selector for passing the control data from the fuse box to the memory arrays; providing a source of alternate control data, external of the integrated circuit; and connecting the source of alternate control data to the control data selector. The design structure further passes the alternate control data from the source thereof, through the control data selector and to the memory arrays to control the redundancy logic of the memory arrays.

  1. A Compact Design of Planar Array Antenna with Fractal Elements for Future Generation Applications

    Ojaroudiparchin, Naser; Shen, Ming; Pedersen, Gert F.

    2016-01-01

    In this paper, a planar phased array fractal antenna for the future fifth generation (5G) applications is presented. The proposed array antenna is designed to operate at 22 GHz. 64 patch antenna elements with coaxial-probe feeds have been used for the proposed design. The antenna elements are based...... on Vicsek fractal geometry where the third iteration patches operate over a wide bandwidth and contribute to improve the efficiency and realized gain performance. The designed planar array has more than 22 dB realized gain and -0.3 dB total efficiency when its beam is tilted to 0 degrees elevation...

  2. A design concept for an MMIC (Monolithic Microwave Integrated Circuit) microstrip phased array

    Lee, Richard Q.; Smetana, Jerry; Acosta, Roberto

    1987-01-01

    A conceptual design for a microstrip phased array with monolithic microwave integrated circuit (MMIC) amplitude and phase controls is described. The MMIC devices used are 20 GHz variable power amplifiers and variable phase shifters recently developed by NASA contractors for applications in future Ka proposed design, which concept is for a general NxN element array of rectangular lattice geometry. Subarray excitation is incorporated in the MMIC phased array design to reduce the complexity of the beam forming network and the number of MMIC components required.

  3. Design and optimization of a self-developing single axis tracking PV array

    Colozza, A.J.

    1992-01-01

    This paper reports on a study performed in order to design a tracking PV array and optimize the design for maximum specific power. The design considerations were minimal deployment time, high reliability and small stowage volume. The array design was self-deployable, from a compact stowage configuration, using a passive pressurized gas deployment mechanism. The array structural components consist of a combination of beams, columns and cables used to deploy and orient a flexible PV blanket. Each structural component of the design was analyzed to determine the size necessary to withstand the various forces it would be subjected to. An optimization was performed to determine the array dimensions and blanket geometry which produce the maximum specific power

  4. Mechanical Design and Development of TES Bolometer Detector Arrays for the Advanced ACTPol Experiment

    Ward, Jonathan T.; Austermann, Jason; Beall, James A.; Choi, Steve K.; Crowley, Kevin T.; Devlin, Mark J.; Duff, Shannon M.; Gallardo, Patricio M.; Henderson, Shawn W.; Ho, Shuay-Pwu Patty; hide

    2016-01-01

    The next generation Advanced ACTPol (AdvACT) experiment is currently underway and will consist of four Transition Edge Sensor (TES) bolometer arrays, with three operating together, totaling 5800 detectors on the sky. Building on experience gained with the ACTPol detector arrays, AdvACT will utilize various new technologies, including 150 mm detector wafers equipped with multichroic pixels, allowing for a more densely packed focal plane. Each set of detectors includes a feedhorn array of stacked silicon wafers which form a spline pro le leading to each pixel. This is then followed by a waveguide interface plate, detector wafer, back short cavity plate, and backshort cap. Each array is housed in a custom designed structure manufactured from high purity copper and then gold plated. In addition to the detector array assembly, the array package also encloses cryogenic readout electronics. We present the full mechanical design of the AdvACT high frequency (HF) detector array package along with a detailed look at the detector array stack assemblies. This experiment will also make use of extensive hardware and software previously developed for ACT, which will be modi ed to incorporate the new AdvACT instruments. Therefore, we discuss the integration of all AdvACT arrays with pre-existing ACTPol infrastructure.

  5. Design and Analysis Tools for Deployable Solar Array Systems, Phase I

    National Aeronautics and Space Administration — Large, lightweight, deployable solar array structures have been identified as a key enabling technology for NASA with analysis and design of these structures being...

  6. Design of Robust Adaptive Array Processors for Non-Stationary Ocean Environments

    Wage, Kathleen E

    2009-01-01

    The overall goal of this project is to design adaptive array processing algorithms that have good transient performance, are robust to mismatch, work with low sample support, and incorporate waveguide...

  7. Design and Analysis Tools for Deployable Solar Array Systems, Phase II

    National Aeronautics and Space Administration — Large, lightweight, deployable solar array structures have been identified as a key enabling technology for NASA with analysis and design of these structures being...

  8. Proceedings of the Flat-Plate Solar Array Project Research Forum on the Design of Flat-Plate Photovoltaic Arrays for Central Stations

    1983-01-01

    The Flat Plate Solar Array Project, focuses on advancing technologies relevant to the design and construction of megawatt level central station systems. Photovoltaic modules and arrays for flat plate central station or other large scale electric power production facilities require the establishment of a technical base that resolves design issues and results in practical and cost effective configurations. Design, qualification and maintenance issues related to central station arrays derived from the engineering and operating experiences of early applications and parallel laboratory reserch activities are investigated. Technical issues are examined from the viewpoint of the utility engineer, architect/engineer and laboratory researcher. Topics on optimum source circuit designs, module insulation design for high system voltages, array safety, structural interface design, measurements, and array operation and maintenance are discussed.

  9. Low power and reliable SRAM memory cell and array design

    Ishibashi, Koichiro

    2011-01-01

    Success in the development of recent advanced semiconductor device technologies is due to the success of SRAM memory cells. This book addresses various issues for designing SRAM memory cells for advanced CMOS technology. To study LSI design, SRAM cell design is the best materials subject because issues about variability, leakage and reliability have to be taken into account for the design.

  10. Practical Consideration Factors to Design Array Configuration of Direction Finding System for Airborne Signal Intelligence

    Jong-Hwan Lee

    2018-01-01

    Full Text Available Airborne signal intelligence (SIGINT systems must be capable of locating radio signal sources. Direction finding (DF to support this capability is an important factor. There are some practical considerations to be taken when designing the array configuration of a DF system for airborne SIGINT systems. This paper summarizes the practical factors when designing the array configuration of the DF system for airborne SIGINT. In particular, it focuses on four areas: antenna consideration factors when installing the DF system for airborne SIGINT from a practical point of view, array configuration methods for airborne communications intelligence and electronic intelligence, and a numerical analysis to select the optimum antenna position for airborne SIGINT.

  11. Design and testing of a combustion-heated nineteen-converter SAVTEC array

    Nyren, T.; Fitzpatrick, G.O.; Korringa, M.; McVey, J.; Sahines, T.

    1984-01-01

    The SAVTEC (Self-Adjusting Versatile Thermionic Energy Converter) is a new design approach for achieving very close (<12μ) interelectrode spacing in a thermionic converter. Techniques were developed for fabricating an array of nineteen SAVTEC converters. The array was incorporated in an SiC protective ''hot shell'' which also served as a radiant heat source for the emitter of each converter. The completed assembly was tested with a specially constructed combustion heat source. Electric output was generated by sixteen of the nineteen converters, despite poor thermal contact in a cooling block, which resulted in high collector temperatures. Details of the array design and test results are described

  12. 2D array design based on Fermat spiral for ultrasound imaging.

    Martínez-Graullera, Oscar; Martín, Carlos J; Godoy, Gregorio; Ullate, Luis G

    2010-02-01

    The main challenge faced by 3D ultrasonic imaging with 2D array transducers is the large number of elements required to achieve an acceptable level of quality in the images. Therefore, the optimisation of the array layout, in order to reduce the number of active elements in the aperture, has been a research topic in the last years. Nowadays, array technology has made viable the production of 2D arrays with larger flexibility on elements size, shape and position, allowing to study other configurations different to the classical matrix organisation, such as circular, archimedes spiral or polygonal layout between others. In this work, the problem of designing an imaging system array with large apertures and a very limited number of active elements (N(e)=128 and N(e)=256) using the Fermat spiral layout has been studied. As summary, a general discussion about the most interesting cases is presented.

  13. Design of a Compact Wideband Antenna Array for Microwave Imaging Applications

    J. Puskely

    2013-12-01

    Full Text Available In the paper, wideband antenna arrays aimed at microwave imaging applications and SAR applications operating at Ka band were designed. The antenna array feeding network is realized by a low-loss SIW technology. Moreover, we have replaced the large feed network comprised of various T and Y junctions by a simple broadband network of compact size to more reduce losses in the substrate integrated waveguide and also save space on the PCB. The designed power 8-way divider is complemented by a wideband substrate integrated waveguide to a grounded coplanar waveguide transition and directly connected to the antenna elements. The measured results of antenna array are consistent with our simulation. Obtained results of the developed array demonstrated improvement compared to previously developed binary feed networks with microstrip or SIW splitters.

  14. Design and analysis of high gain array antenna for wireless communication applications

    Sri Jaya LAKSHMI

    2015-05-01

    Full Text Available The array of antennas generally used for directing the radiated power towards a desired angular sector. Arrays can be used to synthesize a required pattern that cannot be achieved with a single element. The geometrical arrangement, number of elements, phases of the array elements and relative amplitudes depends on the angular pattern. This paper is focused on the issues related to the design and implementation of 4×1 array microstrip antenna with aperture coupled corporate feed for wireless local area network applications. Parametric analysis with change in element spacing is attempted in this work to understand the directional characteristics of the radiation pattern. Gain of more than 14 db and the efficiency more than 93% is achieved from the current design at desired frequency band.

  15. Low Complexity Beampattern Design in MIMO Radars Using Planar Array

    Bouchoucha, Taha; Ahmed, Sajid; Al-Naffouri, Tareq Y.; Alouini, Mohamed-Slim

    2015-01-01

    is then used to generate actual transmitted waveforms. Both steps require constrained optimization, therefore, use iterative and expensive algorithms. In this paper, we provide a closed-form solution to design covariance matrix for the given beampattern using

  16. Highly Directive Reflect Array Antenna Design for Wireless Power Transfer

    2017-04-14

    61102F 6.  AUTHOR(S) Siddhartha Prakash Duttagupta 5d.  PROJECT NUMBER 5e.  TASK NUMBER 5f.   WORK UNIT NUMBER 7.  PERFORMING ORGANIZATION NAME(S) AND...this study by Asian Office of Aerospace Research and Development (AOARD), USA 06 March, 2017 DISTRIBUTION A. Approved for public release...D parabolic design. These include suitability for manufacturing, ease of testing, and flexibility of RAA design for different frequencies

  17. Design of 3x3 Focusing Array for Heavy Ion Driver Final Report on CRADA TC-02082-04

    Martovetsky, N. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2017-09-28

    This memo presents a design of a 3x3 quadrupole array for HIF. It contains 3 D magnetic field computations of the array build with racetrack coils with and without different shields. It is shown that it is possible to have a low error magnetic field in the cells and shield the stray fields to acceptable levels. The array design seems to be a practical solution to any size array for future multi-beam heavy ion fusion drivers.

  18. Magnetic design consideration of a Magnetic Lead Screw with Halbach Array

    Holm, Rasmus Koldborg; Berg, Nick Ilsø; Rasmussen, Peter Omand

    This paper presents the novel design of a Magnetic Lead Screw (MLS) with magnetic thread of Halbach Arrays. The MLS where designed and build, tests indicated a stall force which where 12 % lower than calculated in 3D FE. This is explained by demagnetization of the magnet during stall, the behavio...

  19. Design considerations on a sparse array antenna for Ka-band spaceborne SAR applications

    Jacobs, S.; Bekers, D.; Monni, S.; Otten, M.; Van Rossum, W.; Gerini, G.; Germani, C.; Fortini, D.; Toso, G.

    2015-01-01

    This contribution addresses the problem of designing a sparse active array antenna for spaceborne SAR applications at Ka-band. The main driver for the design is limiting the recurring manufacturing costs associated to the number of active modules, while preserving main performance and insuring a

  20. Robust design of microlenses arrays employing dielectric resonators metasurfaces

    Silvestri, F.; Gerini, G.; Bäumer, S.M.B.

    2017-01-01

    In the last years, much interest has grown around the concept of optical surfaces employing high contrast dielectric resonators. However, a systematic approach for the design of this optical surfaces under particular requirements has never been proposed. In this contribution, we describe this

  1. Analysis and design of arrayed waveguide gratings with MMI couplers.

    Munoz, P; Pastor, D; Capmany, J

    2001-09-24

    We present an extension of the AWG model and design procedure described in [1] to incorporate multimode interference, MMI, couplers. For the first time to our knowledge, a closed formula for the passing bands bandwidth and crosstalk estimation plots are derived.

  2. Novel applications of array comparative genomic hybridization in molecular diagnostics.

    Cheung, Sau W; Bi, Weimin

    2018-05-31

    In 2004, the implementation of array comparative genomic hybridization (array comparative genome hybridization [CGH]) into clinical practice marked a new milestone for genetic diagnosis. Array CGH and single-nucleotide polymorphism (SNP) arrays enable genome-wide detection of copy number changes in a high resolution, and therefore microarray has been recognized as the first-tier test for patients with intellectual disability or multiple congenital anomalies, and has also been applied prenatally for detection of clinically relevant copy number variations in the fetus. Area covered: In this review, the authors summarize the evolution of array CGH technology from their diagnostic laboratory, highlighting exonic SNP arrays developed in the past decade which detect small intragenic copy number changes as well as large DNA segments for the region of heterozygosity. The applications of array CGH to human diseases with different modes of inheritance with the emphasis on autosomal recessive disorders are discussed. Expert commentary: An exonic array is a powerful and most efficient clinical tool in detecting genome wide small copy number variants in both dominant and recessive disorders. However, whole-genome sequencing may become the single integrated platform for detection of copy number changes, single-nucleotide changes as well as balanced chromosomal rearrangements in the near future.

  3. Design of Small CRPA Arrays with Circular Microstrip Loops for Electromagnetically Coupled Feed

    Jun Hur

    2018-04-01

    Full Text Available This paper proposes a design of small controlled reception pattern antenna (CRPA arrays using circular microstrip loops with frequencyinsensitive characteristics. The proposed array consists of seven identical upper and lower circular loops that are electromagnetically coupled, which results in a frequency-insensitive behavior. To demonstrate the feasibility of the proposed feeding mechanism, the proposed array is fabricated, and its antenna characteristics are measured in a full-anechoic chamber. The operating principle of the proposed feeding mechanism is then interpreted using an equivalent circuit model, and the effectiveness of the circular loop shape is demonstrated by calculating near electromagnetic fields in proximity to the radiator. The results confirm that the proposed feeding mechanism is suitable to have frequency-insensitive behavior and induces strong electric and magnetic field strengths for higher radiation gain in extremely small antenna arrays.

  4. Design of UAV-Embedded Microphone Array System for Sound Source Localization in Outdoor Environments

    Kotaro Hoshiba

    2017-11-01

    Full Text Available In search and rescue activities, unmanned aerial vehicles (UAV should exploit sound information to compensate for poor visual information. This paper describes the design and implementation of a UAV-embedded microphone array system for sound source localization in outdoor environments. Four critical development problems included water-resistance of the microphone array, efficiency in assembling, reliability of wireless communication, and sufficiency of visualization tools for operators. To solve these problems, we developed a spherical microphone array system (SMAS consisting of a microphone array, a stable wireless network communication system, and intuitive visualization tools. The performance of SMAS was evaluated with simulated data and a demonstration in the field. Results confirmed that the SMAS provides highly accurate localization, water resistance, prompt assembly, stable wireless communication, and intuitive information for observers and operators.

  5. Design of UAV-Embedded Microphone Array System for Sound Source Localization in Outdoor Environments †

    Hoshiba, Kotaro; Washizaki, Kai; Wakabayashi, Mizuho; Ishiki, Takahiro; Bando, Yoshiaki; Gabriel, Daniel; Nakadai, Kazuhiro; Okuno, Hiroshi G.

    2017-01-01

    In search and rescue activities, unmanned aerial vehicles (UAV) should exploit sound information to compensate for poor visual information. This paper describes the design and implementation of a UAV-embedded microphone array system for sound source localization in outdoor environments. Four critical development problems included water-resistance of the microphone array, efficiency in assembling, reliability of wireless communication, and sufficiency of visualization tools for operators. To solve these problems, we developed a spherical microphone array system (SMAS) consisting of a microphone array, a stable wireless network communication system, and intuitive visualization tools. The performance of SMAS was evaluated with simulated data and a demonstration in the field. Results confirmed that the SMAS provides highly accurate localization, water resistance, prompt assembly, stable wireless communication, and intuitive information for observers and operators. PMID:29099790

  6. Design of UAV-Embedded Microphone Array System for Sound Source Localization in Outdoor Environments.

    Hoshiba, Kotaro; Washizaki, Kai; Wakabayashi, Mizuho; Ishiki, Takahiro; Kumon, Makoto; Bando, Yoshiaki; Gabriel, Daniel; Nakadai, Kazuhiro; Okuno, Hiroshi G

    2017-11-03

    In search and rescue activities, unmanned aerial vehicles (UAV) should exploit sound information to compensate for poor visual information. This paper describes the design and implementation of a UAV-embedded microphone array system for sound source localization in outdoor environments. Four critical development problems included water-resistance of the microphone array, efficiency in assembling, reliability of wireless communication, and sufficiency of visualization tools for operators. To solve these problems, we developed a spherical microphone array system (SMAS) consisting of a microphone array, a stable wireless network communication system, and intuitive visualization tools. The performance of SMAS was evaluated with simulated data and a demonstration in the field. Results confirmed that the SMAS provides highly accurate localization, water resistance, prompt assembly, stable wireless communication, and intuitive information for observers and operators.

  7. Micro solar concentrators: Design and fabrication for microcells arrays

    Jutteau, Sébastien; Paire, Myriam; Proise, Florian; Lombez, Laurent; Guillemoles, Jean-François

    2015-09-01

    In this work we look at a micro-concentrating system adapted to a new type of concentrator photovoltaic material, well known for flate-plate applications, Cu(In,Ga)Se2. Cu(In,Ga)Se2 solar cells are polycrystalline thin film devices that can be deposited by a variety of techniques. We proposed to use a microcell architecture [1], [2], with lateral dimensions varying from a few μm to hundreds of μm, to adapt the film cell to concentration conditions. A 5% absolute efficiency increase on Cu(In,Ga)Se2 microcells at 475 suns has been observed for a final efficiency of 21.3%[3]. We study micro-concentrating systems adapted to the low and middle concentration range, where thin film concentrator cells will lean to substrate fabrication simplification and cost savings. Our study includes optical design, fabrication and experimental tests of prototypes.

  8. Theory and design of compact hybrid microphone arrays on two-dimensional planes for three-dimensional soundfield analysis.

    Chen, Hanchi; Abhayapala, Thushara D; Zhang, Wen

    2015-11-01

    Soundfield analysis based on spherical harmonic decomposition has been widely used in various applications; however, a drawback is the three-dimensional geometry of the microphone arrays. In this paper, a method to design two-dimensional planar microphone arrays that are capable of capturing three-dimensional (3D) spatial soundfields is proposed. Through the utilization of both omni-directional and first order microphones, the proposed microphone array is capable of measuring soundfield components that are undetectable to conventional planar omni-directional microphone arrays, thus providing the same functionality as 3D arrays designed for the same purpose. Simulations show that the accuracy of the planar microphone array is comparable to traditional spherical microphone arrays. Due to its compact shape, the proposed microphone array greatly increases the feasibility of 3D soundfield analysis techniques in real-world applications.

  9. Optimization of ultrasonic arrays design and setting using a differential evolution

    Puel, B.; Chatillon, S.; Calmon, P.; Lesselier, D.

    2011-01-01

    Optimization of both design and setting of phased arrays could be not so easy when they are performed manually via parametric studies. An optimization method based on an Evolutionary Algorithm and numerical simulation is proposed and evaluated. The Randomized Adaptive Differential Evolution has been adapted to meet the specificities of the non-destructive testing applications. In particular, the solution of multi-objective problems is aimed at with the implementation of the concept of pareto-optimal sets of solutions. The algorithm has been implemented and connected to the ultrasonic simulation modules of the CIVA software used as forward model. The efficiency of the method is illustrated on two realistic cases of application: optimization of the position and delay laws of a flexible array inspecting a nozzle, considered as a mono-objective problem; and optimization of the design of a surrounded array and its delay laws, considered as a constrained bi-objective problem. (authors)

  10. Effects of design on cost of flat-plate solar photovoltaic arrays for terrestrial central station power applications

    Tsou, P.; Stolte, W.

    1978-01-01

    The paper examines the impact of module and array designs on the balance-of-plant costs for flat-plate terrestrial central station power applications. Consideration is given to the following types of arrays: horizontal, tandem, augmented, tilt adjusted, and E-W tracking. The life-cycle cost of a 20-year plant life serves as the costing criteria for making design and cost tradeoffs. A tailored code of accounts is developed for determining consistent photovoltaic power plant costs and providing credible photovoltaic system cost baselines for flat-plate module and array designs by costing several varying array design approaches.

  11. Improved Titanium Billet Inspection Sensitivity through Optimized Phased Array Design, Part I: Design Technique, Modeling and Simulation

    Lupien, Vincent; Hassan, Waled; Dumas, Philippe

    2006-01-01

    Reductions in the beam diameter and pulse duration of focused ultrasound for titanium inspections are believed to result in a signal-to-noise ratio improvement for embedded defect detection. It has been inferred from this result that detection limits could be extended to smaller defects through a larger diameter, higher frequency transducer resulting in a reduced beamwidth and pulse duration. Using Continuum Probe Designer TM (Pat. Pending), a transducer array was developed for full coverage inspection of 8 inch titanium billets. The main challenge in realizing a large aperture phased array transducer for billet inspection is ensuring that the number of elements remains within the budget allotted by the driving electronics. The optimization technique implemented by Continuum Probe Designer TM yields an array with twice the aperture but the same number of elements as existing phased arrays for the same application. The unequal area element design was successfully manufactured and validated both numerically and experimentally. Part I of this two-part series presents the design, simulation and modeling steps, while Part II presents the experimental validation and comparative study to multizone

  12. Implementation of the first student-designed PV array in Canada

    Hadlock, C.; DeLoyde, J.; Dhir, T.

    2004-01-01

    This paper is a culmination of a 2-year project involving students, faculty, staff member, and private industry. Solar Technology Education Project (STEP) became the first student-led group to successfully install a 36-panel photovoltaic (PV) array on a Canadian University campus. The fundraising, design, and assembly of the PV array was entirely student driven. The project was completed in January 2004 with the installation of a 2 kW photovoltaic grid-tied array mounted to the roof of the University of Waterloo's Federation Hall, the largest student-run pub in North America. The photovoltaic array was a demonstration project to raise awareness about solar technology and the need for energy efficiency in buildings. It took two years to complete the project, which was implemented in three phases. The first phase was aimed at raising the required capital. The second phase included design and fabrication of the array. The third phase, still ongoing today, is the community outreach phase, which involves educating the surrounding communities about the project, solar technology, and the role of individuals in combating global warming. This paper examines the steps required for the implementation of a successful educational photovoltaic project, using the students' experience as a roadmap. A section highlighting what's next for STEP is also presented as the students attempt to build on the momentum from the project. The aim is to launch a solar thermal project on another University of Waterloo building to move one step closer to the ultimate goal of a sustainable campus

  13. Spatio-spectral color filter array design for optimal image recovery.

    Hirakawa, Keigo; Wolfe, Patrick J

    2008-10-01

    In digital imaging applications, data are typically obtained via a spatial subsampling procedure implemented as a color filter array-a physical construction whereby only a single color value is measured at each pixel location. Owing to the growing ubiquity of color imaging and display devices, much recent work has focused on the implications of such arrays for subsequent digital processing, including in particular the canonical demosaicking task of reconstructing a full color image from spatially subsampled and incomplete color data acquired under a particular choice of array pattern. In contrast to the majority of the demosaicking literature, we consider here the problem of color filter array design and its implications for spatial reconstruction quality. We pose this problem formally as one of simultaneously maximizing the spectral radii of luminance and chrominance channels subject to perfect reconstruction, and-after proving sub-optimality of a wide class of existing array patterns-provide a constructive method for its solution that yields robust, new panchromatic designs implementable as subtractive colors. Empirical evaluations on multiple color image test sets support our theoretical results, and indicate the potential of these patterns to increase spatial resolution for fixed sensor size, and to contribute to improved reconstruction fidelity as well as significantly reduced hardware complexity.

  14. An unified approach for the design of focal-plane arrays in satellite communication

    Zamanifekri, A.; Smolders, A.B.

    2012-01-01

    The aim of this paper is to present a hybrid approach for designing focal plane arrays using commercially available software, considering the fact that the main trade-off in the EM simulation is the accuracy versus computational power. The presented approach is hybrid method based on FDTD/PO. The

  15. Direct Closed-Form Design of Finite Alphabet Constant Envelope Waveforms for Planar Array Beampatterns

    Bouchoucha, Taha

    2015-01-01

    them hard to use in practice especially for real time radar applications. In this paper, we provide a closed-form solution to design the covariance matrix for a given beampattern in the three dimensional space using planar arrays, which is then used

  16. Design of a ring resonator-based optical beam forming network for phased array receive antennas

    van 't Klooster, J.W.J.R.; Roeloffzen, C.G.H.; Meijerink, Arjan; Zhuang, L.; Marpaung, D.A.I.; van Etten, Wim; Heideman, Rene; Leinse, Arne; Schippers, H.; Verpoorte, J.; Wintels, M.

    2008-01-01

    A novel squint-free ring resonator-based optical beam forming network (OBFN) for phased array antennas (PAA) is proposed. It is intended to provide broadband connectivity to airborne platforms via geostationary satellites. In this paper, we present the design of the OBFN and its control system. Our

  17. Chromosomal aberrations in bladder cancer: fresh versus formalin fixed paraffin embedded tissue and targeted FISH versus wide microarray-based CGH analysis.

    Elena Panzeri

    Full Text Available Bladder carcinogenesis is believed to follow two alternative pathways driven by the loss of chromosome 9 and the gain of chromosome 7, albeit other nonrandom copy number alterations (CNAs were identified. However, confirmation studies are needed since many aspects of this model remain unclear and considerable heterogeneity among cases has emerged. One of the purposes of this study was to evaluate the performance of a targeted test (UroVysion assay widely used for the detection of Transitional Cell Carcinoma (TCC of the bladder, in two different types of material derived from the same tumor. We compared the results of UroVysion test performed on Freshly Isolated interphasic Nuclei (FIN and on Formalin Fixed Paraffin Embedded (FFPE tissues from 22 TCCs and we didn't find substantial differences. A second goal was to assess the concordance between array-CGH profiles and the targeted chromosomal profiles of UroVysion assay on an additional set of 10 TCCs, in order to evaluate whether UroVysion is an adequately sensitive method for the identification of selected aneuploidies and nonrandom CNAs in TCCs. Our results confirmed the importance of global genomic screening methods, that is array based CGH, to comprehensively determine the genomic profiles of large series of TCCs tumors. However, this technique has yet some limitations, such as not being able to detect low level mosaicism, or not detecting any change in the number of copies for a kind of compensatory effect due to the presence of high cellular heterogeneity. Thus, it is still advisable to use complementary techniques such as array-CGH and FISH, as the former is able to detect alterations at the genome level not excluding any chromosome, but the latter is able to maintain the individual data at the level of single cells, even if it focuses on few genomic regions.

  18. Mechanical design of a low concentration ratio solar array for a space station application

    Biss, M. S.; Hsu, L.

    1983-01-01

    This paper describes a preliminary study and conceptual design of a low concentration ratio solar array for a space station application with approximately a 100 kW power requirement. The baseline design calls for a multiple series of inverted, truncated, pyramidal optical elements with a geometric concentration ratio (GCR) of 6. It also calls for low life cycle cost, simple on-orbit maintainability, 1984 technology readiness date, and gallium arsenide (GaAs) of silicon (Si) solar cell interchangeability. Due to the large area needed to produce the amount of power required for the baseline space station, a symmetrical wing design, making maximum use of the commonality of parts approach, was taken. This paper will describe the mechanical and structural design of a mass-producible solar array that is very easy to tailor to the needs of the individual user requirement.

  19. Design of a dielectric resonator receive array at 7 Tesla using detunable ceramic resonators

    Ruytenberg, Thomas; Webb, Andrew G.

    2017-11-01

    Ceramic-based dielectric resonators can be used for high frequency magnetic resonance imaging and microscopy. When used as elements in a transmit array, the intrinsically low inter-element coupling allows flexibility in designing different geometric arrangements for different regions-of-interest. However, without being able to detune such resonators, they cannot be used as elements in a receive-only array. Here, we propose and implement a method, based on mode-disruption, for detuning ceramic-based dielectric resonators to enable them to be used as receive-only elements.

  20. Impacts on the Electrical System Economics from Critical Design Factors of Wave Energy Converters and Arrays

    Sharkey, Fergus; Conlon, Michael; Gaughan, Kevin

    2013-01-01

    It is expected that ultimately, like offshore wind farms, electrical systems will make up to a quarter of the overall Capex of wave farms. This is a significant element of cost and consideration must be taken in the design of both individual wave energy converters (WECs) and arrays of WECs to ensure that these costs can be minimised. In a worst case scenario design decisions could increase the cost of the electrical system by several orders and ultimately make the technology uncompetitive. ...

  1. Design and Deployment of a Multichroic Polarimeter Array on the Atacama Cosmology Telescope

    Datta, R.; Austermann, J.; Beall, J. A.; Becker, D.; Coughlin, K. P.; Duff, S. M.; Gallardo, P.A.; Grace, E.; Hasselfield, M.; Henderson, S. W.; hide

    2016-01-01

    We present the design and the preliminary on-sky performance with respect to beams and pass bands of a multichroic polarimeter array covering the 90 and 146 GHz cosmic microwave background bands and its enabling broad-band optical system recently deployed on the Atacama Cosmology Telescope (ACT). The constituent pixels are feedhorn-coupled multichroic polarimeters fabricated at NIST. This array is coupled to the ACT telescope via a set of three silicon lenses incorporating novel broad-band metamaterial anti-reflection coatings. This receiver represents the first multichroic detector array deployed for a CMB experiment and paves the way for the extensive use of multichroic detectors and broad-band optical systems in the next generation of CMB experiments.

  2. Design and Deployment of a Multichroic Polarimeter Array on the Atacama Cosmology Telescope

    Datta, R.; Austermann, J.; Beall, J. A.; Becker, D.; Coughlin, K. P.; Duff, S. M.; Gallardo, P. A.; Grace, E.; Hasselfield, M.; Henderson, S. W.; Hilton, G. C.; Ho, S. P.; Hubmayr, J.; Koopman, B. J.; Lanen, J. V.; Li, D.; McMahon, J.; Munson, C. D.; Nati, F.; Niemack, M. D.; Page, L.; Pappas, C. G.; Salatino, M.; Schmitt, B. L.; Schillaci, A.; Simon, S. M.; Staggs, S. T.; Stevens, J. R.; Vavagiakis, E. M.; Ward, J. T.; Wollack, E. J.

    2016-08-01

    We present the design and the preliminary on-sky performance with respect to beams and passbands of a multichroic polarimeter array covering the 90 and 146 GHz cosmic microwave background bands and its enabling broad-band optical system recently deployed on the Atacama Cosmology Telescope (ACT). The constituent pixels are feedhorn-coupled multichroic polarimeters fabricated at NIST. This array is coupled to the ACT telescope via a set of three silicon lenses incorporating novel broad-band metamaterial anti-reflection coatings. This receiver represents the first multichroic detector array deployed for a CMB experiment and paves the way for the extensive use of multichroic detectors and broad-band optical systems in the next generation of CMB experiments.

  3. Design of a Solar Motor Drive System Fed by a Direct-Connected Photovoltaic Array

    AYDOGMUS, O.

    2012-08-01

    Full Text Available A solar motor pump drive system is modeled and simulated. The proposed drive system does not require any kind of energy storage system and dc-dc converter. The system is connected directly to a photovoltaic (PV array. Thus, a low cost solar system can be achieved. A vector controlled Permanent Magnet Synchronous Motor (PMSM is used as a solar motor to increase the efficiency of system. The motor is designed for a low rated voltage level about 24V. The hill climbing MPPT method is used for balanced the motor power and PV power to obtain a high efficiency. The results are performed by using MATLAB/SimPowerSystem blocks. In addition, the PV array is modeled to allow for the possibility of running as on-line adjustable in simulation environment without using lookup table. The performances of motor, MPPT and drive system are analyzed in different conditions as temperature and irradiation of PV array.

  4. Design and fabrication of magnetically functionalized flexible micropillar arrays for rapid and controllable microfluidic mixing

    Zhou, BingPu; Xu, Wei; Syed, Ahad; Chau, Yeungyeung; Chen, Longqing; Chew, Basil; Yassine, Omar; Wu, Xiaoxiao; Gao, Yibo; Zhang, Jingxian; Xiao, Xiao; Kosel, Jü rgen; Zhang, Xixiang; Yao, Zhaohui; Wen, Weijia

    2015-01-01

    Magnetically functionalized PDMS-based micropillar arrays have been successfully designed, fabricated and implanted for controllable microfluidic mixing. The arrangement of PDMS micropillar arrays inside the microchannel can be flexibly controlled by an external magnetic field. As a consequence, the flow fields inside the microchannel can be regulated at will via magnetic activation conveniently. When a microchannel is implanted with such micropillar arrays, two microstreams can be mixed easily and controllably upon the simple application of an on/off magnetic signal. Mixing efficiencies based on micropillar arrays with different densities were investigated and compared. It was found that micropillar arrays with higher density (i.e. smaller pillar pitch) would render better mixing performance. Our microfluidic system is capable of generating highly reproducible results within many cycles of mixing/non-mixing conversion. We believe that the simple mixing-triggering method together with rapid and controllable mixing control will be extraordinarily valuable for various biological or chemical applications in the future. This journal is © The Royal Society of Chemistry 2015.

  5. Design and fabrication of magnetically functionalized flexible micropillar arrays for rapid and controllable microfluidic mixing

    Zhou, BingPu

    2015-03-25

    Magnetically functionalized PDMS-based micropillar arrays have been successfully designed, fabricated and implanted for controllable microfluidic mixing. The arrangement of PDMS micropillar arrays inside the microchannel can be flexibly controlled by an external magnetic field. As a consequence, the flow fields inside the microchannel can be regulated at will via magnetic activation conveniently. When a microchannel is implanted with such micropillar arrays, two microstreams can be mixed easily and controllably upon the simple application of an on/off magnetic signal. Mixing efficiencies based on micropillar arrays with different densities were investigated and compared. It was found that micropillar arrays with higher density (i.e. smaller pillar pitch) would render better mixing performance. Our microfluidic system is capable of generating highly reproducible results within many cycles of mixing/non-mixing conversion. We believe that the simple mixing-triggering method together with rapid and controllable mixing control will be extraordinarily valuable for various biological or chemical applications in the future. This journal is © The Royal Society of Chemistry 2015.

  6. Design of micro, flexible light-emitting diode arrays and fabrication of flexible electrodes

    Gao, Dan; Wang, Weibiao; Liang, Zhongzhu; Liang, Jingqiu; Qin, Yuxin; Lv, Jinguang

    2016-01-01

    In this study, we design micro, flexible light-emitting diode (LED) array devices. Using theoretical calculations and finite element simulations, we analyze the deformation of the conventional single electrode bar. Through structure optimization, we obtain a three-dimensional (3D), chain-shaped electrode structure, which has a greater bending degree. The optimized electrodes not only have a bigger bend but can also be made to spin. When the supporting body is made of polydimethylsiloxane (PDMS), the maximum bending degree of the micro, flexible LED arrays (4  ×  1 arrays) was approximately 230 µ m; this was obtained using the finite element method. The device (4  ×  1 arrays) can stretch to 15%. This paper describes the fabrication of micro, flexible LED arrays using microelectromechancial (MEMS) technology combined with electroplating technology. Specifically, the isolated grooves are made by dry etching which can isolate and protect the light-emitting units. A combination of MEMS technology and wet etching is used to fabricate the large size spacing. (paper)

  7. Design Concept of Array ECT Sensor for Steam Generator Tubing Inspection

    Cho, Chan Hee; Lee, Tae Hun; Yoo, Hyun Ju [Korea Hydro and Nuclear Power Co. Ltd. CRI, Daejeon (Korea, Republic of)

    2015-05-15

    The eddy current testing, which is one of the nondestructive examination methods, is widely used for the inspection of heat exchangers including steam generator tubing in the nuclear power plant. It uses electromagnetic induction to detect flaws in conductive materials. Two types of eddy current probes are conventionally used for the inspection of steam generator tubing according to the main purpose. One is the bobbin probe technology and the other is the rotating probe. During the inspection, they have restrictions for the flaw detection or the inspection speed. An array probe can be alternative to the bobbin and rotating probes. The design concept of array coils with high sensitivity is described in this paper. It is expected that the eddy current testing using this type of array sensors may provide high detectability and resolution for flaws in steam generator tubing. Eddy current technology has some barriers for the inspection of steam generator tubing in the nuclear power plant. Bobbin probes offer poor circumferential crack detection and rotating probes are time and money consuming due to the mechanical rotation. Array probe inspection technique can replace bobbin and rotating probe techniques due to its sensitivity for flaw detection and inspection speed. In general, circular-shaped coils are considered in an array eddy current probe.

  8. The Implications of Encoder/Modulator/ Phased Array Designs for Future Broadband LEO Communications

    Vanderaar, Mark; Jensen, Chris A.; Terry, John D.

    1997-01-01

    In this paper we summarize the effects of modulation and channel coding on the design of wide angle scan, broadband, phased army antennas. In the paper we perform several trade studies. First, we investigate the amplifier back-off requirement as a function of variability of modulation envelope. Specifically, we contrast constant and non-constant envelope modulations, as well as single and multiple carrier schemes. Additionally, we address the issues an(f concerns of using pulse shaping filters with the above modulation types. Second, we quantify the effects of beam steering on the quality of data, recovery using selected modulation techniques. In particular, we show that the frequency response of the array introduces intersymbol interference for broadband signals and that the mode of operation for the beam steering controller may introduce additional burst or random errors. Finally, we show that the encoder/modulator design must be performed in conjunction with the phased array antenna design.

  9. The design of a second harmonic tangential array interferometer for C-Mod

    Bretz, N.; Jobes, F.; Irby, J.

    1997-01-01

    A design for a tangential array interferometer for C-Mod operating at 1.06 and 0.53 μm is presented. This is a special type of two color interferometer in which a Nd:YAG laser is frequency doubled in a nonlinear crystal. Because the doubling efficiency is imperfect, two frequencies propagate collinearly through the plasma after which the 1.06 μm ray is doubled again mixing in the optical domain with the undoubled ray. The resulting interference is insensitive to path length but is affected by plasma dispersion in the usual way. A typical central fringe shift in C-Mod is expected to be 0.1 endash 1.0, but the absolute and relative accuracy in n e l measurements can be as high as in a conventional interferometer. This design uses a repetitively pulsed laser which is converted to a fan beam crossing the horizontal midplane. The chordal array is defined by internal retroreflectors on the C-Mod midplane which return the beam to the second doubler and a detector array. This interferometer design has beam diameters of a few millimeters and element spacings of a few centimeters, uses a repetitively pulsed, TEM 00 Nd:YAG laser, fiber optic beam transport, commercial components, and a compact optical design which minimizes port space requirements. An optical system design is presented which is based on the performance of a tabletop prototype at Princeton Plasma Physics Laboratory. copyright 1997 American Institute of Physics

  10. Design and performance of single photon APD focal plane arrays for 3-D LADAR imaging

    Itzler, Mark A.; Entwistle, Mark; Owens, Mark; Patel, Ketan; Jiang, Xudong; Slomkowski, Krystyna; Rangwala, Sabbir; Zalud, Peter F.; Senko, Tom; Tower, John; Ferraro, Joseph

    2010-08-01

    ×We describe the design, fabrication, and performance of focal plane arrays (FPAs) for use in 3-D LADAR imaging applications requiring single photon sensitivity. These 32 × 32 FPAs provide high-efficiency single photon sensitivity for three-dimensional LADAR imaging applications at 1064 nm. Our GmAPD arrays are designed using a planarpassivated avalanche photodiode device platform with buried p-n junctions that has demonstrated excellent performance uniformity, operational stability, and long-term reliability. The core of the FPA is a chip stack formed by hybridizing the GmAPD photodiode array to a custom CMOS read-out integrated circuit (ROIC) and attaching a precision-aligned GaP microlens array (MLA) to the back-illuminated detector array. Each ROIC pixel includes an active quenching circuit governing Geiger-mode operation of the corresponding avalanche photodiode pixel as well as a pseudo-random counter to capture per-pixel time-of-flight timestamps in each frame. The FPA has been designed to operate at frame rates as high as 186 kHz for 2 μs range gates. Effective single photon detection efficiencies as high as 40% (including all optical transmission and MLA losses) are achieved for dark count rates below 20 kHz. For these planar-geometry diffused-junction GmAPDs, isolation trenches are used to reduce crosstalk due to hot carrier luminescence effects during avalanche events, and we present details of the crosstalk performance for different operating conditions. Direct measurement of temporal probability distribution functions due to cumulative timing uncertainties of the GmAPDs and ROIC circuitry has demonstrated a FWHM timing jitter as low as 265 ps (standard deviation is ~100 ps).

  11. Design and Implementation of PIR-Array to Reduce Power Consumption for Iraq's Households

    Nidhal Yousif Nasser

    2016-12-01

    Full Text Available The purpose of this study is to design and implement a model using PIR-sensors for motion detection to reduce the electrical power for designing smart home requirements for Iraq households. Reducing energy consumption of home appliances plays an important role in modern designs of smart homes. The PIR (passive infrared sensor has been designed to detect human motion and has many applications especially in security fields. The main concept of the proposed work in this paper has focused on controlling the electrical devices (appliances, based on the location of a person movement inside the home. The design of the circuit control has also been designed to run specific devices located closed to person position in a room by designing PIR sensor array mounted in different angles. The proposed work has been compared with normal state by finding consumption in power (kWh and tariffs belong to MoE-Iraq.

  12. Design of a photovoltaic central power station: flat-plate array

    1984-02-01

    A design for a photovoltaic central power station using fixed flat-panel arrays has been developed. The 100 MW plant is assumed to be located adjacent to the Saguaro Power Station of Arizona Public Service. The design assumes high-efficiency photovoltaic modules using dendritic web cells. The modules are arranged in 5 MW subfields, each with its own power conditioning unit. The photovoltaic output is connected to the existing 115 kV utility switchyard. The site specific design allows detailed cost estimates for engineering, site preparation, and installation. Collector and power conditioning costs have been treated parametrically.

  13. Design of data acquisition system for 2D-ARRAY ionization chamber detector

    He Chaohui; Xing Guilai; Wu Zhifang; Wang Zhentao

    2012-01-01

    The introduction is given on the design and development of data acquisition system for 2D-ARRAY ionization chamber detector, which is used for dose verification of tumor radiotherapy. The paper describes the structure and the principle of the 2D-ARRAY ionization chamber detector system in detail, and focuses on the discussion on the design process of the detector's data acquisition system and the development of data acquisition system which is constituted by preamplifier, preamplifier control board and data acquisition board. The client can setup the parameters of the detector system via TCP/IP and do data processing such as high speed data collection and acquisition, further operation and so on. (authors)

  14. Design, modeling and testing of the Askaryan Radio Array South Pole autonomous renewable power stations

    Besson, D.Z., E-mail: zedlam@ku.edu [Department of Physics and Astronomy, University of Kansas, 1082 Malott Hall, 1251 Wescoe Hall Drive, Lawrence, KS 66045-7582 (United States); Moscow Engineering and Physics Institute, 31 Kashirskaya Highway, Moscow 115409 (Russian Federation); Kennedy, D.M., E-mail: dmkennedy@ku.edu [Department of Physics and Astronomy, University of Kansas, 1082 Malott Hall, 1251 Wescoe Hall Drive, Lawrence, KS 66045-7582 (United States); Ratzlaff, K., E-mail: ratzlaff@ku.edu [Instrumentation Design Laboratory, University of Kansas, 6042 Malott Hall, 1251 Wescoe Hall Drive, Lawrence, KS 66045-7582 (United States); Young, R., E-mail: rwyoung@ku.edu [Instrumentation Design Laboratory, University of Kansas, 6042 Malott Hall, 1251 Wescoe Hall Drive, Lawrence, KS 66045-7582 (United States)

    2014-11-01

    We describe the design, construction and operation of the Askaryan Radio Array (ARA) Autonomous Renewable Power Stations, initially installed at the South Pole in December, 2010 with the goal of providing an independently operating 100 W power source capable of year-round operation in extreme environments. In addition to particle astrophysics applications at the South Pole, such a station can easily be, and has since been, extended to operation elsewhere, as described herein.

  15. Design of a K-Band Transmit Phased Array For Low Earth Orbit Satellite Communications

    Watson, Thomas; Miller, Stephen; Kershner, Dennis; Anzic, Godfrey

    2000-01-01

    The design of a light weight, low cost phased array antenna is presented. Multilayer printed wiring board (PWB) technology is utilized for Radio Frequencies (RF) and DC/Logic manifold distribution. Transmit modules are soldered on one side and patch antenna elements are on the other, allowing the use of automated assembly processes. The 19 GHz antenna has two independently steerable beams, each capable of transferring data at 622 Mbps. A passive, self-contained phase change thermal management system is also presented.

  16. Design of redundant array of independent DVD libraries based on iSCSI

    Chen, Yupeng; Pan, Longfa

    2003-04-01

    This paper presents a new approach to realize the redundant array of independent DVD libraries (RAID-LoIP) by using the iSCSI technology and traditional RAID algorithms. Our design reaches the high performance of optical storage system with following features: large storage size, highly accessing rate, random access, long distance of DVD libraries, block I/O storage, long storage life. Our RAID-LoIP system can be a good solution for broadcasting media asset storage system.

  17. Design, modeling and testing of the Askaryan Radio Array South Pole autonomous renewable power stations

    Besson, D.Z.; Kennedy, D.M.; Ratzlaff, K.; Young, R.

    2014-01-01

    We describe the design, construction and operation of the Askaryan Radio Array (ARA) Autonomous Renewable Power Stations, initially installed at the South Pole in December, 2010 with the goal of providing an independently operating 100 W power source capable of year-round operation in extreme environments. In addition to particle astrophysics applications at the South Pole, such a station can easily be, and has since been, extended to operation elsewhere, as described herein

  18. Camera trap arrays improve detection probability of wildlife: Investigating study design considerations using an empirical dataset.

    O'Connor, Kelly M; Nathan, Lucas R; Liberati, Marjorie R; Tingley, Morgan W; Vokoun, Jason C; Rittenhouse, Tracy A G

    2017-01-01

    Camera trapping is a standard tool in ecological research and wildlife conservation. Study designs, particularly for small-bodied or cryptic wildlife species often attempt to boost low detection probabilities by using non-random camera placement or baited cameras, which may bias data, or incorrectly estimate detection and occupancy. We investigated the ability of non-baited, multi-camera arrays to increase detection probabilities of wildlife. Study design components were evaluated for their influence on wildlife detectability by iteratively parsing an empirical dataset (1) by different sizes of camera arrays deployed (1-10 cameras), and (2) by total season length (1-365 days). Four species from our dataset that represented a range of body sizes and differing degrees of presumed detectability based on life history traits were investigated: white-tailed deer (Odocoileus virginianus), bobcat (Lynx rufus), raccoon (Procyon lotor), and Virginia opossum (Didelphis virginiana). For all species, increasing from a single camera to a multi-camera array significantly improved detection probability across the range of season lengths and number of study sites evaluated. The use of a two camera array increased survey detection an average of 80% (range 40-128%) from the detection probability of a single camera across the four species. Species that were detected infrequently benefited most from a multiple-camera array, where the addition of up to eight cameras produced significant increases in detectability. However, for species detected at high frequencies, single cameras produced a season-long (i.e, the length of time over which cameras are deployed and actively monitored) detectability greater than 0.75. These results highlight the need for researchers to be critical about camera trap study designs based on their intended target species, as detectability for each focal species responded differently to array size and season length. We suggest that researchers a priori identify

  19. Camera trap arrays improve detection probability of wildlife: Investigating study design considerations using an empirical dataset.

    Kelly M O'Connor

    Full Text Available Camera trapping is a standard tool in ecological research and wildlife conservation. Study designs, particularly for small-bodied or cryptic wildlife species often attempt to boost low detection probabilities by using non-random camera placement or baited cameras, which may bias data, or incorrectly estimate detection and occupancy. We investigated the ability of non-baited, multi-camera arrays to increase detection probabilities of wildlife. Study design components were evaluated for their influence on wildlife detectability by iteratively parsing an empirical dataset (1 by different sizes of camera arrays deployed (1-10 cameras, and (2 by total season length (1-365 days. Four species from our dataset that represented a range of body sizes and differing degrees of presumed detectability based on life history traits were investigated: white-tailed deer (Odocoileus virginianus, bobcat (Lynx rufus, raccoon (Procyon lotor, and Virginia opossum (Didelphis virginiana. For all species, increasing from a single camera to a multi-camera array significantly improved detection probability across the range of season lengths and number of study sites evaluated. The use of a two camera array increased survey detection an average of 80% (range 40-128% from the detection probability of a single camera across the four species. Species that were detected infrequently benefited most from a multiple-camera array, where the addition of up to eight cameras produced significant increases in detectability. However, for species detected at high frequencies, single cameras produced a season-long (i.e, the length of time over which cameras are deployed and actively monitored detectability greater than 0.75. These results highlight the need for researchers to be critical about camera trap study designs based on their intended target species, as detectability for each focal species responded differently to array size and season length. We suggest that researchers a priori

  20. Application of high resolution SNP arrays in patients with congenital ...

    TING-YING LEI

    lent oligonucleotide-based array-CGH to determine the exact breakpoints in 14 patients with partial deletions of chromo- some 13q21.1-qter. They were able to refine the smallest deletion region linked to cleft lip/palate (13q31.3–13q33.1). Except for the arrays that measure DNA copy number differ- ences only, SNP arrays, ...

  1. Design of sparse Halbach magnet arrays for portable MRI using a genetic algorithm.

    Cooley, Clarissa Zimmerman; Haskell, Melissa W; Cauley, Stephen F; Sappo, Charlotte; Lapierre, Cristen D; Ha, Christopher G; Stockmann, Jason P; Wald, Lawrence L

    2018-01-01

    Permanent magnet arrays offer several attributes attractive for the development of a low-cost portable MRI scanner for brain imaging. They offer the potential for a relatively lightweight, low to mid-field system with no cryogenics, a small fringe field, and no electrical power requirements or heat dissipation needs. The cylindrical Halbach array, however, requires external shimming or mechanical adjustments to produce B 0 fields with standard MRI homogeneity levels (e.g., 0.1 ppm over FOV), particularly when constrained or truncated geometries are needed, such as a head-only magnet where the magnet length is constrained by the shoulders. For portable scanners using rotation of the magnet for spatial encoding with generalized projections, the spatial pattern of the field is important since it acts as the encoding field. In either a static or rotating magnet, it will be important to be able to optimize the field pattern of cylindrical Halbach arrays in a way that retains construction simplicity. To achieve this, we present a method for designing an optimized cylindrical Halbach magnet using the genetic algorithm to achieve either homogeneity (for standard MRI applications) or a favorable spatial encoding field pattern (for rotational spatial encoding applications). We compare the chosen designs against a standard, fully populated sparse Halbach design, and evaluate optimized spatial encoding fields using point-spread-function and image simulations. We validate the calculations by comparing to the measured field of a constructed magnet. The experimentally implemented design produced fields in good agreement with the predicted fields, and the genetic algorithm was successful in improving the chosen metrics. For the uniform target field, an order of magnitude homogeneity improvement was achieved compared to the un-optimized, fully populated design. For the rotational encoding design the resolution uniformity is improved by 95% compared to a uniformly populated design.

  2. Design considerations for large detector arrays on submillimeter-wave telescopes

    Stark, Antony A.

    2000-07-01

    The emerging technology of large (approximately 10,000 pixel) submillimeter-wave bolometer arrays presents a novel optical design problem -- how can such arrays be fed by diffraction- limited telescope optics where the primary mirror is less than 100,000 wavelengths in diameter? Standard Cassegrain designs for radiotelescope optics exhibit focal surface curvature so large that detectors cannot be placed more than 25 beam diameters from the central ray. The problem is worse for Ritchey-Chretien designs, because these minimize coma while increasing field curvature. Classical aberrations, including coma, are usually dominated by diffraction in submillimeter- wave single dish telescopes. The telescope designer must consider (1) diffraction, (2) aberration, (3) curvature of field, (4) cross-polarization, (5) internal reflections, (6) the effect of blockages, (7) means of beam chopping on- and off-source, (8) gravitational and thermal deformations of the primary mirror, (9) the physical mounting of large detector packages, and (10) the effect of gravity and (11) vibration on those detectors. Simultaneous optimization of these considerations in the case of large detector arrays leads to telescopes that differ considerably from standard radiotelescope designs. Offset optics provide flexibility for mounting detectors, while eliminating blockage and internal reflections. Aberrations and cross-polarization can be the same as on-axis designs having the same diameter and focal length. Trade-offs include the complication of primary mirror homology and an increase in overall cost. A dramatic increase in usable field of view can be achieved using shaped optics. Solutions having one to six mirrors will be discussed, including possible six-mirror design for the proposed South Pole 10 m telescope.

  3. Design for an IO block array in a tile-based FPGA

    Ding Guangxin; Chen Lingdou; Liu Zhongli

    2009-01-01

    A design for an IO block array in a tile-based FPGA is presented. Corresponding with the characteristics of the FPGA, each IO cell is composed of a signal path, local routing pool and configurable input/output buffers. Shared programmable registers in the signal path can be configured for the function of JTAG, without specific boundary scan registers/latches, saving layout area. The local routing pool increases the flexibility of routing and the routability of the whole FPGA. An auxiliary power supply is adopted to increase the performance of the IO buffers at different configured IO standards. The organization of the IO block array is described in an architecture description file, from which the array layout can be accomplished through use of an automated layout assembly tool. This design strategy facilitates the design of FPGAs with different capacities or architectures in an FPGA family series. The bond-out schemes of the same FPGA chip in different packages are also considered. The layout is based on SMIC 0.13 μm logic 1P8M salicide 1.2/2.5 V CMOS technology. Our performance is comparable with commercial SRAM-based FPGAs which use a similar process. (semiconductor integrated circuits)

  4. Design and array signal suggestion of array type pulsed eddy current probe for health monitoring of metal tubes

    Shin, Young Kil [Dept. of Electrical Engineering, Kunsan National University, Kunsan (Korea, Republic of)

    2015-10-15

    An array type probe for monitoring metal tubes is proposed in this paper which utilizes peak value and peak time of a pulsed eddy current(PEC) signal. The probe consists of an array of encircling coils along a tube and the outside of coils is shielded by ferrite to prevent source magnetic fields from directly affecting sensor signals since it is the magnetic fields produced by eddy currents that reflect the condition of metal tubes. The positions of both exciter and sensor coils are consecutively moved automatically so that manual scanning is not necessary. At one position of send-receive coils, peak value and peak time are extracted from a sensor PEC signal and these data are accumulated for all positions to form an array type peak value signal and an array type peak time signal. Numerical simulation was performed using the backward difference method in time and the finite element method for spatial analysis. Simulation results showed that peak value increases and the peak appears earlier as the defect depth or length increases. The proposed array signals are shown to be excellent in reflecting the defect location as well as variations of defect depth and length within the array probe.

  5. Co-Prime Frequency and Aperture Design for HF Surveillance, Wideband Radar Imaging, and Nonstationary Array Processing

    2018-03-01

    to develop novel co-prime sampling and array design strategies that achieve high-resolution estimation of spectral power distributions and signal...by the array geometry and the frequency offset. We overcome this limitation by introducing a novel sparsity-based multi-target localization approach...estimation using a sparse uniform linear array with two CW signals of co-prime frequencies,” IEEE International Workshop on Computational Advances

  6. A French multicenter study of over 700 patients with 22q11 deletions diagnosed using FISH or aCGH.

    Poirsier, Céline; Besseau-Ayasse, Justine; Schluth-Bolard, Caroline; Toutain, Jérôme; Missirian, Chantal; Le Caignec, Cédric; Bazin, Anne; de Blois, Marie Christine; Kuentz, Paul; Catty, Marie; Choiset, Agnès; Plessis, Ghislaine; Basinko, Audrey; Letard, Pascaline; Flori, Elisabeth; Jimenez, Mélanie; Valduga, Mylène; Landais, Emilie; Lallaoui, Hakima; Cartault, François; Lespinasse, James; Martin-Coignard, Dominique; Callier, Patrick; Pebrel-Richard, Céline; Portnoi, Marie-France; Busa, Tiffany; Receveur, Aline; Amblard, Florence; Yardin, Catherine; Harbuz, Radu; Prieur, Fabienne; Le Meur, Nathalie; Pipiras, Eva; Kleinfinger, Pascale; Vialard, François; Doco-Fenzy, Martine

    2016-06-01

    Although 22q11.2 deletion syndrome (22q11.2DS) is the most recurrent human microdeletion syndrome associated with a highly variable phenotype, little is known about the condition's true incidence and the phenotype at diagnosis. We performed a multicenter, retrospective analysis of postnatally diagnosed patients recruited by members of the Association des Cytogénéticiens de Langue Française (the French-Speaking Cytogeneticists Association). Clinical and cytogenetic data on 749 cases diagnosed between 1995 and 2013 were collected by 31 French cytogenetics laboratories. The most frequent reasons for referral of postnatally diagnosed cases were a congenital heart defect (CHD, 48.6%), facial dysmorphism (49.7%) and developmental delay (40.7%). Since 2007 (the year in which array comparative genomic hybridization (aCGH) was introduced for the routine screening of patients with intellectual disability), almost all cases have been diagnosed using FISH (96.1%). Only 15 cases (all with an atypical phenotype) were diagnosed with aCGH; the deletion size ranged from 745 to 2904 kb. The deletion was inherited in 15.0% of cases and was of maternal origin in 85.5% of the latter. This is the largest yet documented cohort of patients with 22q11.2DS (the most commonly diagnosed microdeletion) from the same population. French cytogenetics laboratories diagnosed at least 108 affected patients (including fetuses) per year from among a national population of ∼66 million. As observed for prenatal diagnoses, CHDs were the most frequently detected malformation in postnatal diagnoses. The most common CHD in postnatal diagnoses was an isolated septal defect.

  7. Innovative enclosure dome/observing aperture system design for the MROI Array Telescopes

    Busatta, A.; Marchiori, G.; Mian, S.; Payne, I.; Pozzobon, M.

    2010-07-01

    The close-pack array of the MROI necessitated an original design for the Unit Telescope Enclosure (UTE) at Magdalena Ridge Observatory. The Magdalena Ridge Observatory Interferometer (MROI) is a project which comprises an array of up to ten (10) 1.4m diameter mirror telescopes arranged in a "Y" configuration. Each of these telescopes will be housed inside a Unit Telescope Enclosure (UTE) which are relocatable onto any of 28 stations. The most compact configuration includes all ten telescopes, several of which are at a relative distance of less than 8m center to center from each other. Since the minimum angle of the field of regard is 30° with respect to the horizon, it is difficult to prevent optical blockage caused by adjacent UTEs in this compact array. This paper presents the design constraints inherent in meeting the requirement for the close-pack array. An innovative design enclosure was created which incorporates an unique dome/observing aperture system. The description of this system focuses on how the field of regard requirement led to an unique and highly innovative concept that had to be able to operate in the harsh environmental conditions encountered at an altitude of 10,460ft (3,188m). Finally, we describe the wide use of composites materials and structures (e.g. glass/carbon fibres, sandwich panels etc.) on the aperture system which represents the only way to guarantee adequate thermal and environmental protection, compactness, structural stability and limited power consumption due to reduced mass.

  8. Sparsity-based fast CGH generation using layer-based approach for 3D point cloud model

    Kim, Hak Gu; Jeong, Hyunwook; Ro, Yong Man

    2017-03-01

    Computer generated hologram (CGH) is becoming increasingly important for a 3-D display in various applications including virtual reality. In the CGH, holographic fringe patterns are generated by numerically calculating them on computer simulation systems. However, a heavy computational cost is required to calculate the complex amplitude on CGH plane for all points of 3D objects. This paper proposes a new fast CGH generation based on the sparsity of CGH for 3D point cloud model. The aim of the proposed method is to significantly reduce computational complexity while maintaining the quality of the holographic fringe patterns. To that end, we present a new layer-based approach for calculating the complex amplitude distribution on the CGH plane by using sparse FFT (sFFT). We observe the CGH of a layer of 3D objects is sparse so that dominant CGH is rapidly generated from a small set of signals by sFFT. Experimental results have shown that the proposed method is one order of magnitude faster than recently reported fast CGH generation.

  9. Comparative Genomic Hybridization (CGH) reveals a neo-X chromosome and biased gene movement in stalk-eyed flies (genus Teleopsis).

    Baker, Richard H; Wilkinson, Gerald S

    2010-09-16

    Chromosomal location has a significant effect on the evolutionary dynamics of genes involved in sexual dimorphism, impacting both the pattern of sex-specific gene expression and the rate of duplication and protein evolution for these genes. For nearly all non-model organisms, however, knowledge of chromosomal gene content is minimal and difficult to obtain on a genomic scale. In this study, we utilized Comparative Genomic Hybridization (CGH), using probes designed from EST sequence, to identify genes located on the X chromosome of four species in the stalk-eyed fly genus Teleopsis. Analysis of log(2) ratio values of female-to-male hybridization intensities from the CGH microarrays for over 3,400 genes reveals a strongly bimodal distribution that clearly differentiates autosomal from X-linked genes for all four species. Genotyping of 33 and linkage mapping of 28 of these genes in Teleopsis dalmanni indicate the CGH results correctly identified chromosomal location in all cases. Syntenic comparison with Drosophila indicates that 90% of the X-linked genes in Teleopsis are homologous to genes located on chromosome 2L in Drosophila melanogaster, suggesting the formation of a nearly complete neo-X chromosome from Muller element B in the dipteran lineage leading to Teleopsis. Analysis of gene movement both relative to Drosophila and within Teleopsis indicates that gene movement is significantly associated with 1) rates of protein evolution, 2) the pattern of gene duplication, and 3) the evolution of eyespan sexual dimorphism. Overall, this study reveals that diopsids are a critical group for understanding the evolution of sex chromosomes within Diptera. In addition, we demonstrate that CGH is a useful technique for identifying chromosomal sex-linkage and should be applicable to other organisms with EST or partial genomic information.

  10. Comparative Genomic Hybridization (CGH reveals a neo-X chromosome and biased gene movement in stalk-eyed flies (genus Teleopsis.

    Richard H Baker

    2010-09-01

    Full Text Available Chromosomal location has a significant effect on the evolutionary dynamics of genes involved in sexual dimorphism, impacting both the pattern of sex-specific gene expression and the rate of duplication and protein evolution for these genes. For nearly all non-model organisms, however, knowledge of chromosomal gene content is minimal and difficult to obtain on a genomic scale. In this study, we utilized Comparative Genomic Hybridization (CGH, using probes designed from EST sequence, to identify genes located on the X chromosome of four species in the stalk-eyed fly genus Teleopsis. Analysis of log(2 ratio values of female-to-male hybridization intensities from the CGH microarrays for over 3,400 genes reveals a strongly bimodal distribution that clearly differentiates autosomal from X-linked genes for all four species. Genotyping of 33 and linkage mapping of 28 of these genes in Teleopsis dalmanni indicate the CGH results correctly identified chromosomal location in all cases. Syntenic comparison with Drosophila indicates that 90% of the X-linked genes in Teleopsis are homologous to genes located on chromosome 2L in Drosophila melanogaster, suggesting the formation of a nearly complete neo-X chromosome from Muller element B in the dipteran lineage leading to Teleopsis. Analysis of gene movement both relative to Drosophila and within Teleopsis indicates that gene movement is significantly associated with 1 rates of protein evolution, 2 the pattern of gene duplication, and 3 the evolution of eyespan sexual dimorphism. Overall, this study reveals that diopsids are a critical group for understanding the evolution of sex chromosomes within Diptera. In addition, we demonstrate that CGH is a useful technique for identifying chromosomal sex-linkage and should be applicable to other organisms with EST or partial genomic information.

  11. Note: The design of thin gap chamber simulation signal source based on field programmable gate array

    Hu, Kun; Wang, Xu; Li, Feng; Jin, Ge; Lu, Houbing; Liang, Futian

    2015-01-01

    The Thin Gap Chamber (TGC) is an important part of ATLAS detector and LHC accelerator. Targeting the feature of the output signal of TGC detector, we have designed a simulation signal source. The core of the design is based on field programmable gate array, randomly outputting 256-channel simulation signals. The signal is generated by true random number generator. The source of randomness originates from the timing jitter in ring oscillators. The experimental results show that the random number is uniform in histogram, and the whole system has high reliability

  12. Design and fabrication of a micro PZT cantilever array actuator for applications in fluidic systems

    Kim, H.; In, C.; Yoon, Gil Ho

    2005-01-01

    In this article, a micro cantilever array actuated by PZT films is designed and fabricated for micro fluidic systems. The design features for maximizing tip deflections and minimizing fluid leakage are described. The governing equation of the composite PZT cantilever is derived and the actuating......, dielectric constant, and dielectric loss. Tip deflections of 12 mu m at 5 V are measured, which agreed well with the predicted value. The 18 mu l/s leakage rate of air was observed at a pressure difference of 1000 Pa. Micro cooler is introduced, and its possible application to micro compressor is discussed....

  13. Optimized emission in nanorod arrays through quasi-aperiodic inverse design.

    Anderson, P Duke; Povinelli, Michelle L

    2015-06-01

    We investigate a new class of quasi-aperiodic nanorod structures for the enhancement of incoherent light emission. We identify one optimized structure using an inverse design algorithm and the finite-difference time-domain method. We carry out emission calculations on both the optimized structure as well as a simple periodic array. The optimized structure achieves nearly perfect light extraction while maintaining a high spontaneous emission rate. Overall, the optimized structure can achieve a 20%-42% increase in external quantum efficiency relative to a simple periodic design, depending on material quality.

  14. Bridging the gap from prenatal karyotyping to whole-genome array comparative genomic hybridization in Hong Kong: survey on knowledge and acceptance of health-care providers and pregnant women.

    Cheng, Hiu Yee Heidi; Kan, Anita Sik-Yau; Hui, Pui Wah; Lee, Chin Peng; Tang, Mary Hoi Yin

    2017-12-01

    The use of array comparative genomic hybridization (aCGH) has been increasingly widespread. The challenge of integration of this technology into prenatal diagnosis was the interpretation of results and communicating findings of unclear clinical significance. This study assesses the knowledge and acceptance of prenatal aCGH in Hong Kong obstetricians and pregnant women. The aim is to identify the needs and gaps before implementing the replacement of karyotyping with aCGH. Questionnaires with aCGH information in the form of pamphlets were sent by post to obstetrics and gynecology doctors. For the pregnant women group, a video presentation, pamphlets on aCGH and a self-administered questionnaire were provided at the antenatal clinic. The perception of aCGH between doctors and pregnant women was similar. Doctors not choosing aCGH were more concerned about the difficulty in counseling of variants of unknown significance and adult-onset disease in pregnant women, whereas pregnant women not choosing aCGH were more concerned about the increased waiting time leading to increased anxiety. Prenatal aCGH is perceived as a better test by both doctors and patients. Counseling support, training, and better understanding and communication of findings of unclear clinical significance are necessary to improve doctor-patient experience.

  15. Design and fabrication of a bidimentional microbolometer array for Terahertz detection characterized at different temperatures

    Orduna-Diaz, A [CIBA-Tlaxcala, IPN, Tlaxcala, Tlax.(Mexico); Castillo-DomInguez, E; Torres-Jacome, A; De la Hidalga-Wade, F J; Trevino-Palacios, C G, E-mail: abdu@inaoep.mx [Instituto Nacional de Astrofisica, Optica y Electronica, Apdo. Postal 51 y 216, Puebla, Pue. 72000 (Mexico)

    2011-01-01

    We present the design, micromachining and characterization of a bidimentional bolometer array for radiation detection in the 0.7-1.5 THz frequency range. The detector is based on a boron doped amorphous silicon film (a-Si-B:H). The film optimized for sensitivity enhancement was obtained using 500 sccm diborane flow with 95 nm thickness. The sensing layer was deposited using plasma enhanced chemical vapor deposition (PECVD) technique at low frequency on a 0.45 {mu}m thick silicon nitride membrane sustained by a micromachined frame in crystalline silicon. The design consists of four 5x5 bolometer arrays made by conventional lithography. The bolometer active area is 660 {mu}m x 420 {mu}m and the detector will operate as a focal plane array. The current-voltage characteristics present an ohmic behaviour; the temperature coefficient of resistance (TCR) was obtained by measuring the bolometer performance from room temperature down to liquid nitrogen temperature. The responsivity was measured under illumination from a black body radiating at 300, 500, 700, 900 and 1100 deg. C, obtaining a value of R =1.17 x 10{sup -2} A/W with a dark current of 4.43 x 10{sup -9} A

  16. Design and Fabrication of an Experimental Microheater Array Powder Sintering Printer

    Holt, Nicholas; Zhou, Wenchao

    2018-03-01

    Microheater array powder sintering (MAPS) is a novel additive manufacturing process that uses an array of microheaters to selectively sinter powder particles. MAPS shows great promise as a new method of printing flexible electronics by enabling digital curing of conductive inks on a variety of substrates. For MAPS to work effectively, a microscale air gap needs to be maintained between the heater array and the conductive ink. In this article, we present an experimental MAPS printer with air gap control for printing conductive circuits. First, we discuss design aspects necessary to implement MAPS. An analysis is performed to validate that the design can maintain the desired air gap between the microheaters and the sintering layer, which consists of a silver nanoparticle ink. The printer is tested by printing conductive lines on a flexible plastic substrate with silver nanoparticle ink. Results show MAPS performs on par with or better than the existing fabrication methods for printed electronics in terms of both the print quality (conductivity of the printed line) and print speed, which shows MAPS' great promise as a competitive new method for digital production of printed electronics.

  17. Design and Development of the Space Technology 5 (ST5) Solar Arrays

    Lyons, John; Fatemi, Navid; Gamica, Robert; Sharma, Surya; Senft, Donna; Maybery, Clay

    2005-01-01

    The National Aeronautics and Space Administration's (NASA's) Space Technology 5 (ST5) is designed to flight-test the concept of miniaturized 'small size" satellites and innovative technologies in Earth's magnetosphere. Three satellites will map the intensity and direction of the magnetic fields within the inner magnetosphere. Due to the small area available for the solar arrays, and to meet the mission power requirements, very high-efficiency multijunction solar cells were selected to power the spacecraft built by NASA Goddard Space Flight Center (GSFC). This was done in partnership with the Air Force Research Lab (AFRL) through the Dual-Use Science and Technology (DUS&T) program. Emcore's InGaP/lnGaAs/Ge Advanced triple-junction (ATJ) solar cells, exhibiting an average air mass zero (AMO) efficiency of 28.0% (one-sun, 28 C), were used to populate the arrays. Each spacecraft employs 8 identical solar panels (total area of about 0.3 square meters), with 15 large-area solar cells per panel. The requirement for power is to support on-orbit average load of 13.5 W at 8.4 V, with plus or minus 5% off pointing. The details of the solar array design, development and qualification considerations, as well as ground electrical performance & shadowing analysis results are presented.

  18. Direct Closed-Form Design of Finite Alphabet Constant Envelope Waveforms for Planar Array Beampatterns

    Bouchoucha, Taha

    2015-05-01

    Multiple Input Multiple Output (MIMO) radar systems has attracted lately a lot of attention thanks to its advantage over the classical phased array radar systems. We site among these advantages the improvement of parametric identifiability, achievement of higher spatial resolution and design of complex beampatterns. In colocated multiple-input multiple-output radar systems, it is usually desirable to steer transmitted power in the region-of-interest in order to increase the Signal to Noise Ratio (SNR) and reduce any undesired signal and thus improve the detection process. This problem is also known as transmit beampattern design. To achieve this goal, conventional methods optimize the waveform covariance matrix, R, for the desired beampattern, which is then used to generate the actual transmitted waveforms. Both steps require constrained optimization. Most of the existing methods use iterative algorithms to solve these problems, therefore their computational complexity is very high which makes them hard to use in practice especially for real time radar applications. In this paper, we provide a closed-form solution to design the covariance matrix for a given beampattern in the three dimensional space using planar arrays, which is then used to derive a novel closed-form algorithm to directly design the finite-alphabet constant-envelope waveforms. The proposed algorithm exploits the two-dimensional discrete Fourier transform which is implemented using fast Fourier transform algorithm. Consequently, the computational complexity of the proposed beampattern solution is very low allowing it to be used for large arrays to change the beampattern in real time. We also show that the number of required snapshots in each waveform depends on the beampattern and that it is less than the total number of transmit antennas. In addition, we show that the proposed waveform design method can be used with non symmetric beampatterns. The performance of our proposed algorithm compares

  19. Synthesis of Gold Nanoparticles to Capture Lifelike Proteins: Application on the Multichannel Sensor Array Design

    Yumin Leng

    2018-01-01

    Full Text Available The chemical elements of proteins are similar to that of DNA (e.g., C, H, O, and N, and DNA shows different knotted architectures. So we imagine that proteins may show a wealth of highly complex structures, especially when proteins interact with each other. The imagination was proved by synthesizing gold nanoparticles (GNPs to capture the lifelike protein structures. The optical responses (i.e., color of as-prepared GNPs are found to be characteristic to a given protein (or heavy metal ion. Based on the “three colors” principle of Thomas Young, we extracted the red, green, and blue (RGB alterations of as-synthesized GNPs to fabricate multichannel sensor arrays for proteins (or heavy metal ions discrimination. The designed multichannel sensor arrays demonstrate possibilities in semiquantitative analysis of multiple analytes (e.g., proteins and heavy metal ions. This work is believed to open new opportunities for GNPs-based label-free sensing.

  20. Design and Assembly of DNA Nano-Objects and 2D DNA Origami Arrays

    Liu, Wenyan

    DNA, which plays a central role in biology as the carrier of genetic information, is also an excellent candidate for structural nanotechnology. Researches have proven that a variety of complicated DNA assemblies, such as objects, 2D & 3D crystals, and nanomechanical devices, can be fabricated through the combination of robust branched DNA motifs and sticky ends. This dissertation focuses on the design and construction of DNA nano--objects and 2D DNA origami arrays. In this dissertation, we first describe the formation of a triangular species that has four strands per edge, held together by PX interactions. We demonstrate by nondenaturing gel electrophoresis and by atomic force microscopy (AFM) that we can combine a partial triangle with other strands to form a robust four--stranded molecule. By combining them with a novel three--domain molecule, we also demonstrate by AFM that these triangles can be self--assembled into a linear array. Second, we demonstrate our attempts to design and self--assemble 2D DNA origami arrays using several different strategies. Specifically, we introduce the self--assembly of 2D DNA origami lattices using a symmetric cross--like design. This design strategy resulted in a well--ordered woven latticework array with edge dimensions of 2--3 mum. This size is likely to be large enough to connect bottom-up methods of patterning with top--down approaches. Third, we illustrate the design and construction of DNA nano--objects for exploring the substrate preferences of topoisomerase (topo) II. We designed and fabricated four double rhombus--like DNA molecules, each of which contains a different conformation of crossover in the middle, as possible substrates to establish the structural preferences for topo II. We characterized the formation of each substrate molecule by gel electrophoresis. Finally, we study the effect of M13 DNA knotting on the formation of the DNA origami tiles. We demonstrate by atomic force microscopy (AFM) that knotted M13

  1. Heavy Ion Transient Characterization of a Photobit Hardened-by-Design Active Pixel Sensor Array

    Marshall, Paul W.; Byers, Wheaton B.; Conger, Christopher; Eid, El-Sayed; Gee, George; Jones, Michael R.; Marshall, Cheryl J.; Reed, Robert; Pickel, Jim; Kniffin, Scott

    2002-01-01

    This paper presents heavy ion data on the single event transient (SET) response of a Photobit active pixel sensor (APS) four quadrant test chip with different radiation tolerant designs in a standard 0.35 micron CMOS process. The physical design techniques of enclosed geometry and P-channel guard rings are used to design the four N-type active photodiode pixels as described in a previous paper. Argon transient measurements on the 256 x 256 chip array as a function of incident angle show a significant variation in the amount of charge collected as well as the charge spreading dependent on the pixel type. The results are correlated with processing and design information provided by Photobit. In addition, there is a large degree of statistical variability between individual ion strikes. No latch-up is observed up to an LET of 106 MeV/mg/sq cm.

  2. The Square Kilometre Array Science Data Processor. Preliminary compute platform design

    Broekema, P.C.; Nieuwpoort, R.V. van; Bal, H.E.

    2015-01-01

    The Square Kilometre Array is a next-generation radio-telescope, to be built in South Africa and Western Australia. It is currently in its detailed design phase, with procurement and construction scheduled to start in 2017. The SKA Science Data Processor is the high-performance computing element of the instrument, responsible for producing science-ready data. This is a major IT project, with the Science Data Processor expected to challenge the computing state-of-the art even in 2020. In this paper we introduce the preliminary Science Data Processor design and the principles that guide the design process, as well as the constraints to the design. We introduce a highly scalable and flexible system architecture capable of handling the SDP workload

  3. Design of readout drivers for ATLAS pixel detectors using field programmable gate arrays

    Sivasubramaniyan, Sriram

    Microstrip detectors are an integral patt of high energy physics research . Special protocols are used to transmit the data from these detectors . To readout the data from such detectors specialized instrumentation have to be designed . To achieve this task, creative and innovative high speed algorithms were designed simulated and implemented in Field Programmable gate arrays, using CAD/CAE tools. The simulation results indicated that these algorithms would be able to perform all the required tasks quickly and efficiently. This thesis describes the design of data acquisition system called the Readout Drivers (ROD) . It focuses on the ROD data path for ATLAS Pixel detectors. The data path will be an integrated part of Readout Drivers setup to decode the data from the silicon micro strip detectors and pixel detectors. This research also includes the design of Readout Driver controller. This Module is used to control the operation of the ROD. This module is responsible for the operation of the Pixel decoders bas...

  4. Bi-Axial Solar Array Drive Mechanism: Design, Build and Environmental Testing

    Scheidegger, Noemy; Ferris, Mark; Phillips, Nigel

    2014-01-01

    The development of the Bi-Axial Solar Array Drive Mechanism (BSADM) presented in this paper is a demonstration of SSTL's unique space manufacturing approach that enables performing rapid development cycles for cost-effective products that meet ever-challenging mission requirements: The BSADM is designed to orient a solar array wing towards the sun, using its first rotation axis to track the sun, and its second rotation axis to compensate for the satellite orbit and attitude changes needed for a successful payload operation. The tight development schedule, with manufacture of 7 Flight Models within 1.5 year after kick-off, is offset by the risk-reduction of using qualified key component-families from other proven SSTL mechanisms. This allowed focusing the BSADM design activities on the mechanism features that are unique to the BSADM, and having an Engineering Qualification Model (EQM) built 8 months after kick-off. The EQM is currently undergoing a full environmental qualification test campaign. This paper presents the BSADM design approach that enabled meeting such a challenging schedule, its design particularities, and the ongoing verification activities.

  5. Design, Fabrication, and Testing of Active Skin Antenna with 3D Printing Array Framework

    Jinzhu Zhou

    2017-01-01

    Full Text Available An active skin antenna with structural load-bearing and electromagnetic functions is usually installed in the structural surface of mobile vehicles such as aircrafts, warships, and high-speed train. This paper presents the design, fabrication, and testing of a novel active skin antenna which consists of an encapsulation shell, antenna skin, and RF and beam control circuits. The antenna skin which consists of the facesheet, honeycomb, array framework, and microstrip antenna elements was designed by using Bayesian optimization, in order to improve the design efficiency. An active skin antenna prototype with 32 microstrip antenna elements was fabricated by using a hybrid manufacturing method. In this method, 3D printing technology was applied to fabricate the array framework, and the different layers were bonded to form the final antenna skin by using traditional composite process. Some experimental testing was conducted, and the testing results validate the feasibility the proposed antenna skin structure. The proposed design and fabrication technique is suitable for the development of conformal load-bearing antenna or smart skin antenna installed in the structural surface of aircraft, warships, and armored vehicles.

  6. Design and optimization of arrays of neodymium iron boron-based magnets for magnetic tweezers applications

    Zacchia, Nicholas A.; Valentine, Megan T. [Department of Mechanical Engineering and Materials Research Laboratory, University of California, Santa Barbara, California 93106 (United States)

    2015-05-15

    We present the design methodology for arrays of neodymium iron boron (NdFeB)-based magnets for use in magnetic tweezers devices. Using finite element analysis (FEA), we optimized the geometry of the NdFeB magnet as well as the geometry of iron yokes designed to focus the magnetic fields toward the sample plane. Together, the magnets and yokes form a magnetic array which is the basis of the magnetic tweezers device. By systematically varying 15 distinct shape parameters, we determined those features that maximize the magnitude of the magnetic field gradient as well as the length scale over which the magnetic force operates. Additionally, we demonstrated that magnetic saturation of the yoke material leads to intrinsic limitations in any geometric design. Using this approach, we generated a compact and light-weight magnetic tweezers device that produces a high field gradient at the image plane in order to apply large forces to magnetic beads. We then fabricated the optimized yoke and validated the FEA by experimentally mapping the magnetic field of the device. The optimization data and iterative FEA approach outlined here will enable the streamlined design and construction of specialized instrumentation for force-sensitive microscopy.

  7. Design and optimization of arrays of neodymium iron boron-based magnets for magnetic tweezers applications.

    Zacchia, Nicholas A; Valentine, Megan T

    2015-05-01

    We present the design methodology for arrays of neodymium iron boron (NdFeB)-based magnets for use in magnetic tweezers devices. Using finite element analysis (FEA), we optimized the geometry of the NdFeB magnet as well as the geometry of iron yokes designed to focus the magnetic fields toward the sample plane. Together, the magnets and yokes form a magnetic array which is the basis of the magnetic tweezers device. By systematically varying 15 distinct shape parameters, we determined those features that maximize the magnitude of the magnetic field gradient as well as the length scale over which the magnetic force operates. Additionally, we demonstrated that magnetic saturation of the yoke material leads to intrinsic limitations in any geometric design. Using this approach, we generated a compact and light-weight magnetic tweezers device that produces a high field gradient at the image plane in order to apply large forces to magnetic beads. We then fabricated the optimized yoke and validated the FEA by experimentally mapping the magnetic field of the device. The optimization data and iterative FEA approach outlined here will enable the streamlined design and construction of specialized instrumentation for force-sensitive microscopy.

  8. Design and optical analyses of an arrayed microfluidic tunable prism panel for enhancing solar energy collection

    Narasimhan, Vinayak; Jiang, Dongyue; Park, Sung-Yong

    2016-01-01

    Highlights: • We present an arrayed tunable prism panel enabling wide tracking and high solar concentration. • A microfluidic technology allows a low-cost, lightweight and precise solar tracking system. • Our prism panel enables high solar concentration up to 2032× factor. • Various liquid prism configurations (stacked prism arrays) and optical materials are considered. • Their impacts on solar beam steering, reflection losses and beam concentration are studied. - Abstract: We present the design and optical analyses of an arrayed microfluidic tunable prism panel that enables wide solar tracking and high solar concentration while minimizing energy loss. Each of the liquid prism modules is implemented by a microfluidic (i.e. non-mechanical) technology based on electrowetting for adaptive solar beam steering. Therefore the proposed platform offers a low-cost, lightweight and precise solar tracking system while obviating the need for bulky and heavy mechanical moving parts essentially required for a conventional motor-driven solar tracker. In this paper, various liquid prism configurations in terms of design (single, double, triple and quad-stacked prism arrays) as well as optical materials are considered and their impact on optical performance aspects such as solar beam steering, reflection losses and beam concentration is studied. Our system is able to achieve a wide solar tracking covering the whole-day movement of the Sun and a reflection loss below 4.4% with a Rayleigh’s film for a quad-stacked prism configuration. Furthermore, an arrayed prism panel is proposed to increase the aperture area and thus allows for the collection of large amounts of sunlight. Our simulation study based on the optical design software, ZEMAX, indicates that the prism panel is capable of high solar concentration up to 2032× factor even without conventional solar tracking devices. We also deal with dispersion characteristics of the materials and their corresponding effect on

  9. Design of UAVs-Based 3D Antenna Arrays for a Maximum Performance in Terms of Directivity and SLL

    Jesus Garza

    2016-01-01

    Full Text Available This paper presents a design of UAVs-based 3D antenna arrays for a maximum performance in terms of directivity and side lobe level (SLL. This paper illustrates how to model the UAVs formation flight using 3D nonuniform antenna arrays. This design of 3D antenna arrays considers the optimization of the positions of the antenna elements to model the UAVs formation flight. In this case, a disk patch antenna is chosen to be used as element in each UAV. The disk patch antenna is formulated by the well-known cavity model. The synthesis process is carried out by the method of Differential Evolution for Multiobjective Optimization (DEMO. Furthermore, a comparison of the performance of 3D nonuniform antenna arrays is provided with respect to the most conventional arrays (circular, planar, linear, and the cubic for UAVs formation flight.

  10. Design, Fabrication, and Implementation of an Array-Type MEMS Piezoresistive Intelligent Pressure Sensor System

    Jiahong Zhang

    2018-02-01

    Full Text Available To meet the radiosonde requirement of high sensitivity and linearity, this study designs and implements a monolithically integrated array-type piezoresistive intelligent pressure sensor system which is made up of two groups of four pressure sensors with the pressure range of 0–50 kPa and 0–100 kPa respectively. First, theoretical models and ANSYS (version 14.5, Canonsburg, PA, USA finite element method (FEM are adopted to optimize the parameters of array sensor structure. Combing with FEM stress distribution results, the size and material characteristics of the array-type sensor are determined according to the analysis of the sensitivity and the ratio of signal to noise (SNR. Based on the optimized parameters, the manufacture and packaging of array-type sensor chips are then realized by using the standard complementary metal-oxide-semiconductor (CMOS and microelectromechanical system (MEMS process. Furthermore, an intelligent acquisition and processing system for pressure and temperature signals is achieved. The S3C2440A microprocessor (Samsung, Seoul, Korea is regarded as the core part which can be applied to collect and process data. In particular, digital signal storage, display and transmission are realized by the application of a graphical user interface (GUI written in QT/E. Besides, for the sake of compensating the temperature drift and nonlinear error, the data fusion technique is proposed based on a wavelet neural network improved by genetic algorithm (GA-WNN for average measuring signal. The GA-WNN model is implemented in hardware by using a S3C2440A microprocessor. Finally, the results of calibration and test experiments achieved with the temperature ranges from −20 to 20 °C show that: (1 the nonlinear error and the sensitivity of the array-type pressure sensor are 8330 × 10−4 and 0.052 mV/V/kPa in the range of 0–50 kPa, respectively; (2 the nonlinear error and the sensitivity are 8129 × 10−4 and 0.020 mV/V/kPa in the

  11. Design and fabrication of two-dimensional semiconducting bolometer arrays for HAWC and SHARC-II

    Voellmer, George M.; Allen, Christine A.; Amato, Michael J.; Babu, Sachidananda R.; Bartels, Arlin E.; Benford, Dominic J.; Derro, Rebecca J.; Dowell, C. D.; Harper, D. A.; Jhabvala, Murzy D.; Moseley, S. H.; Rennick, Timothy; Shirron, Peter J.; Smith, W. W.; Staguhn, Johannes G.

    2003-02-01

    The High resolution Airborne Wideband Camera (HAWC) and the Submillimeter High Angular Resolution Camera II (SHARC II) will use almost identical versions of an ion-implanted silicon bolometer array developed at the National Aeronautics and Space Administration's Goddard Space Flight Center (GSFC). The GSFC "Pop-Up" Detectors (PUD's) use a unique folding technique to enable a 12 × 32-element close-packed array of bolometers with a filling factor greater than 95 percent. A kinematic Kevlar suspension system isolates the 200 mK bolometers from the helium bath temperature, and GSFC - developed silicon bridge chips make electrical connection to the bolometers, while maintaining thermal isolation. The JFET preamps operate at 120 K. Providing good thermal heat sinking for these, and keeping their conduction and radiation from reaching the nearby bolometers, is one of the principal design challenges encountered. Another interesting challenge is the preparation of the silicon bolometers. They are manufactured in 32-element, planar rows using Micro Electro Mechanical Systems (MEMS) semiconductor etching techniques, and then cut and folded onto a ceramic bar. Optical alignment using specialized jigs ensures their uniformity and correct placement. The rows are then stacked to create the 12 × 32-element array. Engineering results from the first light run of SHARC II at the Caltech Submillimeter Observatory (CSO) are presented.

  12. Design of area array CCD image acquisition and display system based on FPGA

    Li, Lei; Zhang, Ning; Li, Tianting; Pan, Yue; Dai, Yuming

    2014-09-01

    With the development of science and technology, CCD(Charge-coupled Device) has been widely applied in various fields and plays an important role in the modern sensing system, therefore researching a real-time image acquisition and display plan based on CCD device has great significance. This paper introduces an image data acquisition and display system of area array CCD based on FPGA. Several key technical challenges and problems of the system have also been analyzed and followed solutions put forward .The FPGA works as the core processing unit in the system that controls the integral time sequence .The ICX285AL area array CCD image sensor produced by SONY Corporation has been used in the system. The FPGA works to complete the driver of the area array CCD, then analog front end (AFE) processes the signal of the CCD image, including amplification, filtering, noise elimination, CDS correlation double sampling, etc. AD9945 produced by ADI Corporation to convert analog signal to digital signal. Developed Camera Link high-speed data transmission circuit, and completed the PC-end software design of the image acquisition, and realized the real-time display of images. The result through practical testing indicates that the system in the image acquisition and control is stable and reliable, and the indicators meet the actual project requirements.

  13. Design of coated standing nanowire array solar cell performing beyond the planar efficiency limits

    Zeng, Yang; Ye, Qinghao; Shen, Wenzhong, E-mail: wzshen@sjtu.edu.cn [Institute of Solar Energy, and Key Laboratory of Artificial Structures and Quantum Control (Ministry of Education), Department of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai 200240 (China)

    2016-05-28

    The single standing nanowire (SNW) solar cells have been proven to perform beyond the planar efficiency limits in both open-circuit voltage and internal quantum efficiency due to the built-in concentration and the shifting of the absorption front. However, the expandability of these nano-scale units to a macro-scale photovoltaic device remains unsolved. The main difficulty lies in the simultaneous preservation of an effective built-in concentration in each unit cell and a broadband high absorption capability of their array. Here, we have provided a detailed theoretical guideline for realizing a macro-scale solar cell that performs furthest beyond the planar limits. The key lies in a complementary design between the light-trapping of the single SNWs and that of the photonic crystal slab formed by the array. By tuning the hybrid HE modes of the SNWs through the thickness of a coaxial dielectric coating, the optimized coated SNW array can sustain an absorption rate over 97.5% for a period as large as 425 nm, which, together with the inherited carrier extraction advantage, leads to a cell efficiency increment of 30% over the planar limit. This work has demonstrated the viability of a large-size solar cell that performs beyond the planar limits.

  14. Mechanical design of SST-GATE, a dual-mirror telescope for the Cherenkov Telescope Array

    Dournaux, Jean-Laurent; Huet, Jean-Michel; Amans, Jean-Philippe; Dumas, Delphine; Laporte, Philippe; Sol, Hélène; Blake, Simon

    2014-07-01

    The Cherenkov Telescope Array (CTA) project aims to create the next generation Very High Energy (VHE) gamma-ray telescope array. It will be devoted to the observation of gamma rays over a wide band of energy, from a few tens of GeV to more than 100 TeV. Two sites are foreseen to view the whole sky where about 100 telescopes, composed of three different classes, related to the specific energy region to be investigated, will be installed. Among these, the Small Size class of Telescopes, SSTs, are devoted to the highest energy region, to beyond 100 TeV. Due to the large number of SSTs, their unit cost is an important parameter. At the Observatoire de Paris, we have designed a prototype of a Small Size Telescope named SST-GATE, based on the dual-mirror Schwarzschild-Couder optical formula, which has never before been implemented in the design of a telescope. Over the last two years, we developed a mechanical design for SST-GATE from the optical and preliminary mechanical designs made by the University of Durham. The integration of this telescope is currently in progress. Since the early stages of mechanical design of SST-GATE, finite element method has been used employing shape and topology optimization techniques to help design several elements of the telescope. This allowed optimization of the mechanical stiffness/mass ratio, leading to a lightweight and less expensive mechanical structure. These techniques and the resulting mechanical design are detailed in this paper. We will also describe the finite element analyses carried out to calculate the mechanical deformations and the stresses in the structure under observing and survival conditions.

  15. Clinical Utility of Array Comparative Genomic Hybridization for Detection of Chromosomal Abnormalities in Pediatric Acute Lymphoblastic Leukemia

    Rabin, Karen R.; Man, Tsz-Kwong; Yu, Alexander; Folsom, Matthew R.; Zhao, Yi-Jue; Rao, Pulivarthi H.; Plon, Sharon E.; Naeem, Rizwan C.

    2014-01-01

    Background Accurate detection of recurrent chromosomal abnormalities is critical to assign patients to risk-based therapeutic regimens for pediatric acute lymphoblastic leukemia (ALL). Procedure We investigated the utility of array comparative genomic hybridization (aCGH) for detection of chromosomal abnormalities compared to standard clinical evaluation with karyotype and fluorescent in-situ hybridization (FISH). Fifty pediatric ALL diagnostic bone marrows were analyzed by bacterial artificial chromosome (BAC) array, and findings compared to standard clinical evaluation. Results Sensitivity of aCGH was 79% to detect karyotypic findings other than balanced translocations, which cannot be detected by aCGH because they involve no copy number change. aCGH also missed abnormalities occurring in subclones constituting less than 25% of cells. aCGH detected 44 additional abnormalities undetected or misidentified by karyotype, 21 subsequently validated by FISH, including abnormalities in 4 of 10 cases with uninformative cytogenetics. aCGH detected concurrent terminal deletions of both 9p and 20q in three cases, in two of which the 20q deletion was undetected by karyotype. A narrow region of loss at 7p21 was detected in two cases. Conclusions An array with increased BAC density over regions important in ALL, combined with PCR for fusion products of balanced translocations, could minimize labor- and time-intensive cytogenetic assays and provide key prognostic information in the approximately 35% of cases with uninformative cytogenetics. PMID:18253961

  16. Design and implementation of a low-cost multichannel seismic noise recorder for array measurements

    Soler-Llorens, Juan Luis; Juan Giner-Caturla, Jose; Molina-Palacios, Sergio; Galiana-Merino, Juan Jose; Rosa-Herranz, Julio; Agea-Medina, Noelia

    2017-04-01

    Soil characterization is the starting point for seismic hazard studies. Currently, the methods based on ambient noise measurements are very used because they are non-invasive methods and relatively easy to implement in urban areas. Among these methods, the analysis of array measurements provides the dispersion curve and subsequently the shear-wave velocity profile associated to the site under study. In this case, we need several sensors recording simultaneously and a data acquisition system with one channel by sensor, what can become the complete equipment unaffordable for small research groups. In this work, we have designed and implemented a low-cost multichannel ambient noise recorder for array measurements. The complete system is based on Arduino, an open source electronic development platform, which allows recording 12 differential input channels simultaneously. Besides, it is complemented with a conditioning circuit that includes an anti-aliasing filter and a selectable gain between 0 and 40dB. The data acquisition is set up through a user-friendly graphical user interface. It is important to note that the electronic scheme as well as the programming code are open hardware and software, respectively, so it allows other researchers to suite the system to their particular requirements. The developed equipment has been tested at several sites around the province of Alicante (southeast of Spain), where the soil characteristics are well-known from previous studies. Array measurements have been taken and after that, the recorded data have been analysed using the frequency-wavenumber (f-k) and the extended spatial autocorrelation (ESAC) methods. The comparison of the obtained dispersion curves with the ones obtained in previous studies shows the suitability of the implemented low-cost system for array measurements.

  17. Design and fabrication of adjustable red-green-blue LED light arrays for plant research

    Kenitz J Dustin

    2005-08-01

    Full Text Available Abstract Background Although specific light attributes, such as color and fluence rate, influence plant growth and development, researchers generally cannot control the fine spectral conditions of artificial plant-growth environments. Plant growth chambers are typically outfitted with fluorescent and/or incandescent fixtures that provide a general spectrum that is accommodating to the human eye and not necessarily supportive to plant development. Many studies over the last several decades, primarily in Arabidopsis thaliana, have clearly shown that variation in light quantity, quality and photoperiod can be manipulated to affect growth and control developmental transitions. Light emitting diodes (LEDs has been used for decades to test plant responses to narrow-bandwidth light. LEDs are particularly well suited for plant growth chambers, as they have an extraordinary life (about 100,000 hours, require little maintenance, and use negligible energy. These factors render LED-based light strategies particularly appropriate for space-biology as well as terrestrial applications. However, there is a need for a versatile and inexpensive LED array platform where individual wavebands can be specifically tuned to produce a series of light combinations consisting of various quantities and qualities of individual wavelengths. Two plans are presented in this report. Results In this technical report we describe the practical construction of tunable red-green-blue LED arrays to support research in plant growth and development. Two light fixture designs and corresponding circuitry are presented. The first is well suited for a laboratory environment for use in a finite area with small plants, such as Arabidopsis. The second is expandable and appropriate for growth chambers. The application of these arrays to early plant developmental studies has been validated with assays of hypocotyl growth inhibition/promotion and phototropic curvature in Arabidopsis seedlings

  18. Application of source biasing technique for energy efficient DECODER circuit design: memory array application

    Gupta, Neha; Parihar, Priyanka; Neema, Vaibhav

    2018-04-01

    Researchers have proposed many circuit techniques to reduce leakage power dissipation in memory cells. If we want to reduce the overall power in the memory system, we have to work on the input circuitry of memory architecture i.e. row and column decoder. In this research work, low leakage power with a high speed row and column decoder for memory array application is designed and four new techniques are proposed. In this work, the comparison of cluster DECODER, body bias DECODER, source bias DECODER, and source coupling DECODER are designed and analyzed for memory array application. Simulation is performed for the comparative analysis of different DECODER design parameters at 180 nm GPDK technology file using the CADENCE tool. Simulation results show that the proposed source bias DECODER circuit technique decreases the leakage current by 99.92% and static energy by 99.92% at a supply voltage of 1.2 V. The proposed circuit also improves dynamic power dissipation by 5.69%, dynamic PDP/EDP 65.03% and delay 57.25% at 1.2 V supply voltage.

  19. VLSI design of an RSA encryption/decryption chip using systolic array based architecture

    Sun, Chi-Chia; Lin, Bor-Shing; Jan, Gene Eu; Lin, Jheng-Yi

    2016-09-01

    This article presents the VLSI design of a configurable RSA public key cryptosystem supporting the 512-bit, 1024-bit and 2048-bit based on Montgomery algorithm achieving comparable clock cycles of current relevant works but with smaller die size. We use binary method for the modular exponentiation and adopt Montgomery algorithm for the modular multiplication to simplify computational complexity, which, together with the systolic array concept for electric circuit designs effectively, lower the die size. The main architecture of the chip consists of four functional blocks, namely input/output modules, registers module, arithmetic module and control module. We applied the concept of systolic array to design the RSA encryption/decryption chip by using VHDL hardware language and verified using the TSMC/CIC 0.35 m 1P4 M technology. The die area of the 2048-bit RSA chip without the DFT is 3.9 × 3.9 mm2 (4.58 × 4.58 mm2 with DFT). Its average baud rate can reach 10.84 kbps under a 100 MHz clock.

  20. Evaluation of a miniature microscope objective designed for fluorescence array microscopy detection of Mycobacterium tuberculosis.

    McCall, Brian; Olsen, Randall J; Nelles, Nicole J; Williams, Dawn L; Jackson, Kevin; Richards-Kortum, Rebecca; Graviss, Edward A; Tkaczyk, Tomasz S

    2014-03-01

    A prototype miniature objective that was designed for a point-of-care diagnostic array microscope for detection of Mycobacterium tuberculosis and previously fabricated and presented in a proof of concept is evaluated for its effectiveness in detecting acid-fast bacteria. To evaluate the ability of the microscope to resolve submicron features and details in the image of acid-fast microorganisms stained with a fluorescent dye, and to evaluate the accuracy of clinical diagnoses made with digital images acquired with the objective. The lens prescription data for the microscope design are presented. A test platform is built by combining parts of a standard microscope, a prototype objective, and a digital single-lens reflex camera. Counts of acid-fast bacteria made with the prototype objective are compared to counts obtained with a standard microscope over matched fields of view. Two sets of 20 smears, positive and negative, are diagnosed by 2 pathologists as sputum smear positive or sputum smear negative, using both a standard clinical microscope and the prototype objective under evaluation. The results are compared to a reference diagnosis of the same sample. More bacteria are counted in matched fields of view in digital images taken with the prototype objective than with the standard clinical microscope. All diagnostic results are found to be highly concordant. An array microscope built with this miniature lens design will be able to detect M tuberculosis with high sensitivity and specificity.

  1. Tunable Absorption System based on magnetorheological elastomers and Halbach array: design and testing

    Bocian, Mirosław; Kaleta, Jerzy; Lewandowski, Daniel, E-mail: daniel.lewandowski@pwr.edu.pl; Przybylski, Michał

    2017-08-01

    Highlights: • Construction of a Tunable Absorption System incorporating MRE has been done. • For system control by magnetic field a double circular Halbach array has been used. • Significant changes of the TSAs natural frequency and damping has been obtained. - Abstract: In this paper, the systematic design, construction and testing of a Tunable Absorption System (TAS) incorporating magnetorheological elastomer (MRE) has been investigated. The TAS has been designed for energy absorption and mitigation of vibratory motions from an impact excitation. The main advantage of the designed TAS is that it has the ability to change and adapt to working conditions. Tunability can be realised through a change in the magnetic field caused by the change of an internal arrangement of permanent magnets within a double dipolar circular Halbach array. To show the capabilities of the tested system, experiments based on an impulse excitation have been performed. Significant changes of the TASs natural frequency and damping characteristics have been obtained. By incorporating magnetic tunability within the TAS a significant qualitative and quantitative change in the devices mechanical properties and performance were obtained.

  2. Closed-form Solution to Directly Design FACE Waveforms for Beampatterns Using Planar Array

    Bouchoucha, Taha; Ahmed, Sajid; Al-Naffouri, Tareq Y.; Alouini, Mohamed-Slim

    2015-01-01

    In multiple-input multiple-output radar systems, it is usually desirable to steer transmitted power in the region-of-interest. To do this, conventional methods optimize the waveform covariance matrix, R, for the desired beampattern, which is then used to generate actual transmitted waveforms. Both steps require constrained optimization, therefore, use iterative algorithms. The main challenges encountered in the existing approaches are the computational complexity and the design of waveforms to use in practice. In this paper, we provide a closed-form solution to design covariance matrix for the given beampattern using the planar array, which is then used to derive a novel closed-form algorithm to directly design the finite-alphabet constant-envelope (FACE) waveforms. The proposed algorithm exploits the two-dimensional fast-Fourier-transform. The performance of our proposed algorithm is compared with existing methods that are based on semi-definite quadratic programming with the advantage of a considerably reduced complexity.

  3. Closed-form Solution to Directly Design FACE Waveforms for Beampatterns Using Planar Array

    Bouchoucha, Taha

    2015-04-19

    In multiple-input multiple-output radar systems, it is usually desirable to steer transmitted power in the region-of-interest. To do this, conventional methods optimize the waveform covariance matrix, R, for the desired beampattern, which is then used to generate actual transmitted waveforms. Both steps require constrained optimization, therefore, use iterative algorithms. The main challenges encountered in the existing approaches are the computational complexity and the design of waveforms to use in practice. In this paper, we provide a closed-form solution to design covariance matrix for the given beampattern using the planar array, which is then used to derive a novel closed-form algorithm to directly design the finite-alphabet constant-envelope (FACE) waveforms. The proposed algorithm exploits the two-dimensional fast-Fourier-transform. The performance of our proposed algorithm is compared with existing methods that are based on semi-definite quadratic programming with the advantage of a considerably reduced complexity.

  4. Design of hybrid two-dimensional and three-dimensional nanostructured arrays for electronic and sensing applications

    Ko, Hyunhyub

    This dissertation presents the design of organic/inorganic hybrid 2D and 3D nanostructured arrays via controlled assembly of nanoscale building blocks. Two representative nanoscale building blocks such as carbon nanotubes (one-dimension) and metal nanoparticles (zero-dimension) are the core materials for the study of solution-based assembly of nanostructured arrays. The electrical, mechanical, and optical properties of the assembled nanostructure arrays have been investigated for future device applications. We successfully demonstrated the prospective use of assembled nanostructure arrays for electronic and sensing applications by designing flexible carbon nanotube nanomembranes as mechanical sensors, highly-oriented carbon nanotubes arrays for thin-film transistors, and gold nanoparticle arrays for SERS chemical sensors. In first section, we fabricated highly ordered carbon nanotube (CNT) arrays by tilted drop-casting or dip-coating of CNT solution on silicon substrates functionalized with micropatterned self-assembled monolayers. We further exploited the electronic performance of thin-film transistors based on highly-oriented, densely packed CNT micropatterns and showed that the carrier mobility is largely improved compared to randomly oriented CNTs. The prospective use of Raman-active CNTs for potential mechanical sensors has been investigated by studying the mechano-optical properties of flexible carbon nanotube nanomembranes, which contain freely-suspended carbon nanotube array encapsulated into ultrathin (optical waveguide properties of nano-canals. We demonstrated the ability of this SERS substrate for trace level sensing of nitroaromatic explosives by detecting down to 100 zeptogram (˜330 molecules) of DNT.

  5. Salivary gland carcinosarcoma: oligonucleotide array CGH reveals similar genomic profiles in epithelial and mesenchymal components

    Vékony, H.; Leemans, C.R.; Ylstra, B.; Meijer, G.A.; van der Waal, I.; Bloemena, E.

    2009-01-01

    In this study, we present a case of parotid gland de novo carcinosarcoma. Salivary gland carcinosarcoma (or true malignant mixed tumor) is a rare biphasic neoplasm, composed of both malignant epithelial and malignant mesenchymal components. It is yet unclear whether these two phenotypes occur by

  6. Detection of Genetic Alterations in Breast Sentinel Lymph Node by Array-CGH

    Cavalli, Luciane R

    2005-01-01

    The sentinel lymph node (SLN) is the first node in the mammary gland to harbor malignant cells in breast tumors with metastasis, and SLN positivity is an indication for axillary lymph node dissection...

  7. Detection of Genetic Alterations in Breast Sentinel Lymph Node by Array-CGH

    Cavalli, Luciane R

    2006-01-01

    The sentinel lymph node (SLN) is the first node in the mammary gland to harbor malignant cells in breast tumors with metastasis, and SLN positivity is an indication for axillary lymph node dissection...

  8. Comparative cytogenetic characterization of primary canine melanocytic lesions using array CGH and fluorescence in situ hybridization

    Poorman, Kelsey; Borst, Luke; Moroff, Scott; Roy, Siddharth; Labelle, Philippe; Motsinger-Reif, Alison; Breen, Matthew

    2014-01-01

    Melanocytic lesions originating from the oral mucosa or cutaneous epithelium are common in the general dog population, with up to 100,000 diagnoses each year in the USA. Oral melanoma is the most frequent canine neoplasm of the oral cavity, exhibiting a highly aggressive course. Cutaneous melanocytomas occur frequently, but rarely develop into a malignant form. Despite the differential prognosis, it has been assumed that subtypes of melanocytic lesions represent the same disease. To address t...

  9. Fine-tiling array CGH to improve diagnostics for alpha- and beta-thalassemia rearrangements

    Phylipsen, M.; Chaibunruang, A.; Vogelaar, I.P.; Balak, J.R.; Schaap, R.A.; Ariyurek, Y.; Fucharoen, S.; den Dunnen, J.T.; Giordano, P.C.; Bakker, E.; Harteveld, C.L.

    2012-01-01

    Implementation of multiplex ligation-dependent probe amplification (MLPA) for thalassemia causing deletions has lead to the detection of new rearrangements. Knowledge of the exact breakpoint sequences should give more insight into the molecular mechanisms underlying these rearrangements, and would

  10. Design of oligonucleotides for microarrays and perspectives for design of multi-transcriptome arrays

    Nielsen, Henrik Bjørn; Wernersson, Rasmus; Knudsen, Steen

    2003-01-01

    with an overview of these parameters. We present here a flexible tool named OligoWiz for designing oligonucleotides for multiple purposes. OligoWiz presents a set of parameter scores in a graphical interface to facilitate an overview for the user. Additional custom parameter scores can easily be added......Optimal design of oligonucleotides for microarrays involves tedious and laborious work evaluating potential oligonucleotides relative to a series of parameters. The currently available tools for this purpose are limited in their flexibility and do not present the oligonucleotide designer...... to the program to extend the default parameters: homology, DeltaTm, low-complexity, position and GATC-only. Furthermore we present an analysis of the limitations in designing oligonucleotide sets that can detect transcripts from multiple organisms. OligoWiz is available at www.cbs.dtu.dk/services/OligoWiz/....

  11. Topology optimization for design of segmented permanent magnet arrays with ferromagnetic materials

    Lee, Jaewook; Yoon, Minho; Nomura, Tsuyoshi; Dede, Ercan M.

    2018-03-01

    This paper presents multi-material topology optimization for the co-design of permanent magnet segments and iron material. Specifically, a co-design methodology is proposed to find an optimal border of permanent magnet segments, a pattern of magnetization directions, and an iron shape. A material interpolation scheme is proposed for material property representation among air, permanent magnet, and iron materials. In this scheme, the permanent magnet strength and permeability are controlled by density design variables, and permanent magnet magnetization directions are controlled by angle design variables. In addition, a scheme to penalize intermediate magnetization direction is proposed to achieve segmented permanent magnet arrays with discrete magnetization directions. In this scheme, permanent magnet strength is controlled depending on magnetization direction, and consequently the final permanent magnet design converges into permanent magnet segments having target discrete directions. To validate the effectiveness of the proposed approach, three design examples are provided. The examples include the design of a dipole Halbach cylinder, magnetic system with arbitrarily-shaped cavity, and multi-objective problem resembling a magnetic refrigeration device.

  12. Design of acoustic logging signal source of imitation based on field programmable gate array

    Zhang, K; Ju, X D; Lu, J Q; Men, B Y

    2014-01-01

    An acoustic logging signal source of imitation is designed and realized, based on the Field Programmable Gate Array (FPGA), to improve the efficiency of examining and repairing acoustic logging tools during research and field application, and to inspect and verify acoustic receiving circuits and corresponding algorithms. The design of this signal source contains hardware design and software design,and the hardware design uses an FPGA as the control core. Four signals are made first by reading the Random Access Memory (RAM) data which are inside the FPGA, then dealing with the data by digital to analog conversion, amplification, smoothing and so on. Software design uses VHDL, a kind of hardware description language, to program the FPGA. Experiments illustrate that the ratio of signal to noise for the signal source is high, the waveforms are stable, and also its functions of amplitude adjustment, frequency adjustment and delay adjustment are in accord with the characteristics of real acoustic logging waveforms. These adjustments can be used to imitate influences on sonic logging received waveforms caused by many kinds of factors such as spacing and span of acoustic tools, sonic speeds of different layers and fluids, and acoustic attenuations of different cementation planes. (paper)

  13. Design of acoustic logging signal source of imitation based on field programmable gate array

    Zhang, K.; Ju, X. D.; Lu, J. Q.; Men, B. Y.

    2014-08-01

    An acoustic logging signal source of imitation is designed and realized, based on the Field Programmable Gate Array (FPGA), to improve the efficiency of examining and repairing acoustic logging tools during research and field application, and to inspect and verify acoustic receiving circuits and corresponding algorithms. The design of this signal source contains hardware design and software design,and the hardware design uses an FPGA as the control core. Four signals are made first by reading the Random Access Memory (RAM) data which are inside the FPGA, then dealing with the data by digital to analog conversion, amplification, smoothing and so on. Software design uses VHDL, a kind of hardware description language, to program the FPGA. Experiments illustrate that the ratio of signal to noise for the signal source is high, the waveforms are stable, and also its functions of amplitude adjustment, frequency adjustment and delay adjustment are in accord with the characteristics of real acoustic logging waveforms. These adjustments can be used to imitate influences on sonic logging received waveforms caused by many kinds of factors such as spacing and span of acoustic tools, sonic speeds of different layers and fluids, and acoustic attenuations of different cementation planes.

  14. Design and optimization of multi-class series-parallel linear electromagnetic array artificial muscle.

    Li, Jing; Ji, Zhenyu; Shi, Xuetao; You, Fusheng; Fu, Feng; Liu, Ruigang; Xia, Junying; Wang, Nan; Bai, Jing; Wang, Zhanxi; Qin, Xiansheng; Dong, Xiuzhen

    2014-01-01

    Skeletal muscle exhibiting complex and excellent precision has evolved for millions of years. Skeletal muscle has better performance and simpler structure compared with existing driving modes. Artificial muscle may be designed by analyzing and imitating properties and structure of skeletal muscle based on bionics, which has been focused on by bionic researchers, and a structure mode of linear electromagnetic array artificial muscle has been designed in this paper. Half sarcomere is the minimum unit of artificial muscle and electromagnetic model has been built. The structural parameters of artificial half sarcomere actuator were optimized to achieve better movement performance. Experimental results show that artificial half sarcomere actuator possesses great motion performance such as high response speed, great acceleration, small weight and size, robustness, etc., which presents a promising application prospect of artificial half sarcomere actuator.

  15. Formulation Development and Evaluation of Hybrid Nanocarrier for Cancer Therapy: Taguchi Orthogonal Array Based Design

    Rakesh K. Tekade

    2013-01-01

    Full Text Available Taguchi orthogonal array design is a statistical approach that helps to overcome limitations associated with time consuming full factorial experimental design. In this study, the Taguchi orthogonal array design was applied to establish the optimum conditions for bovine serum albumin (BSA nanocarrier (ANC preparation. Taguchi method with L9 type of robust orthogonal array design was adopted to optimize the experimental conditions. Three key dependent factors namely, BSA concentration (% w/v, volume of BSA solution to total ethanol ratio (v : v, and concentration of diluted ethanolic aqueous solution (% v/v, were studied at three levels 3%, 4%, and 5% w/v; 1 : 0.75, 1 : 0.90, and 1 : 1.05 v/v; 40%, 70%, and 100% v/v, respectively. The ethanolic aqueous solution was used to impart less harsh condition for desolvation and attain controlled nanoparticle formation. The interaction plot studies inferred the ethanolic aqueous solution concentration to be the most influential parameter that affects the particle size of nanoformulation. This method (BSA, 4% w/v; volume of BSA solution to total ethanol ratio, 1 : 0.90 v/v; concentration of diluted ethanolic solution, 70% v/v was able to successfully develop Gemcitabine (G loaded modified albumin nanocarrier (M-ANC-G of size 25.07±2.81 nm (ζ=-23.03±1.015 mV as against to 78.01±4.99 nm (ζ=-24.88±1.37 mV using conventional method albumin nanocarrier (C-ANC-G. Hybrid nanocarriers were generated by chitosan layering (solvent gelation technique of respective ANC to form C-HNC-G and M-HNC-G of sizes 125.29±5.62 nm (ζ=12.01±0.51 mV and 46.28±2.21 nm (ζ=15.05±0.39 mV, respectively. Zeta potential, entrapment, in vitro release, and pH-based stability studies were investigated and influence of formulation parameters are discussed. Cell-line-based cytotoxicity assay (A549 and H460 cells and cell internalization assay (H460 cell line were performed to assess the

  16. Driver-Array Based Flat-Panel Loudspeakers: Theoretical Background and Design Guidelines

    Anderson, David Allan

    This thesis relates to the simulation and design of flat-panel loudspeakers using moving-coil driver elements. A brief history of the industry is given, including a collection of products and patents from 1925 until the present, an overview of research papers, and a discussion of current products available. The mechanics of bending flat panels are developed with respect to localized driving forces, both in the frequency domain and the time domain as an impulse response. These simulations are compared to measurements on prototype panels. Additional resonant elements influence the behavior of the system: an optional ported rear enclosure and the resonant characteristics of the drivers. The governing equations for these systems are derived and solutions are implemented using equivalent mechanical circuits and numerical methods. The idea of using driver arrays to independently actuate modes of the panel is discussed at length with respect to modal addressability, modal spillover, and experimental validation. The numerical approach to determining the optimal driver placement for a given set of modes is derived and experimentally validated. An investigation of the acoustic behavior of flat panel loudspeakers is presented, using mechanical simulation results to predict the acoustic radiation. The simulations are compared to measurements and found to accurately predict important mechanical and acoustical behaviors. It is demonstrated that a driver array, with the proper biasing, is capable of creating a flat panel loudspeaker which acts more like a piston than a "diffuse radiator" flat panel loudspeaker. The techniques of "Modal Crossover Networks" are introduced, which use multi-band filters to bias the driver array differently for different frequency bands, optimized for audio reproduction. The question of how many drivers are necessary for a modal crossover network is addressed and found to be dependent on the estimated quality factor (Q) of the panel material and edge

  17. Computer holography: 3D digital art based on high-definition CGH

    Matsushima, K; Arima, Y; Nishi, H; Yamashita, H; Yoshizaki, Y; Ogawa, K; Nakahara, S

    2013-01-01

    Our recent works of high-definition computer-generated holograms (CGH) and the techniques used for the creation, such as the polygon-based method, silhouette method and digitized holography, are summarized and reviewed in this paper. The concept of computer holography is proposed in terms of integrating and crystalizing the techniques into novel digital art.

  18. Design Methodology of a Dual-Halbach Array Linear Actuator with Thermal-Electromagnetic Coupling.

    Eckert, Paulo Roberto; Flores Filho, Aly Ferreira; Perondi, Eduardo; Ferri, Jeferson; Goltz, Evandro

    2016-03-11

    This paper proposes a design methodology for linear actuators, considering thermal and electromagnetic coupling with geometrical and temperature constraints, that maximizes force density and minimizes force ripple. The method allows defining an actuator for given specifications in a step-by-step way so that requirements are met and the temperature within the device is maintained under or equal to its maximum allowed for continuous operation. According to the proposed method, the electromagnetic and thermal models are built with quasi-static parametric finite element models. The methodology was successfully applied to the design of a linear cylindrical actuator with a dual quasi-Halbach array of permanent magnets and a moving-coil. The actuator can produce an axial force of 120 N and a stroke of 80 mm. The paper also presents a comparative analysis between results obtained considering only an electromagnetic model and the thermal-electromagnetic coupled model. This comparison shows that the final designs for both cases differ significantly, especially regarding its active volume and its electrical and magnetic loading. Although in this paper the methodology was employed to design a specific actuator, its structure can be used to design a wide range of linear devices if the parametric models are adjusted for each particular actuator.

  19. Generation of a genomic tiling array of the human Major Histocompatibility Complex (MHC and its application for DNA methylation analysis

    Ottaviani Diego

    2008-05-01

    Full Text Available Abstract Background The major histocompatibility complex (MHC is essential for human immunity and is highly associated with common diseases, including cancer. While the genetics of the MHC has been studied intensively for many decades, very little is known about the epigenetics of this most polymorphic and disease-associated region of the genome. Methods To facilitate comprehensive epigenetic analyses of this region, we have generated a genomic tiling array of 2 Kb resolution covering the entire 4 Mb MHC region. The array has been designed to be compatible with chromatin immunoprecipitation (ChIP, methylated DNA immunoprecipitation (MeDIP, array comparative genomic hybridization (aCGH and expression profiling, including of non-coding RNAs. The array comprises 7832 features, consisting of two replicates of both forward and reverse strands of MHC amplicons and appropriate controls. Results Using MeDIP, we demonstrate the application of the MHC array for DNA methylation profiling and the identification of tissue-specific differentially methylated regions (tDMRs. Based on the analysis of two tissues and two cell types, we identified 90 tDMRs within the MHC and describe their characterisation. Conclusion A tiling array covering the MHC region was developed and validated. Its successful application for DNA methylation profiling indicates that this array represents a useful tool for molecular analyses of the MHC in the context of medical genomics.

  20. Design of DSP-based high-power digital solar array simulator

    Zhang, Yang; Liu, Zhilong; Tong, Weichao; Feng, Jian; Ji, Yibo

    2013-12-01

    To satisfy rigid performance specifications, a feedback control was presented for zoom optical lens plants. With the increasing of global energy consumption, research of the photovoltaic(PV) systems get more and more attention. Research of the digital high-power solar array simulator provides technical support for high-power grid-connected PV systems research.This paper introduces a design scheme of the high-power digital solar array simulator based on TMS320F28335. A DC-DC full-bridge topology was used in the system's main circuit. The switching frequency of IGBT is 25kHz.Maximum output voltage is 900V. Maximum output current is 20A. Simulator can be pre-stored solar panel IV curves.The curve is composed of 128 discrete points .When the system was running, the main circuit voltage and current values was feedback to the DSP by the voltage and current sensors in real-time. Through incremental PI,DSP control the simulator in the closed-loop control system. Experimental data show that Simulator output voltage and current follow a preset solar panels IV curve. In connection with the formation of high-power inverter, the system becomes gridconnected PV system. The inverter can find the simulator's maximum power point and the output power can be stabilized at the maximum power point (MPP).

  1. Design of Hybrid Nanostructural Arrays to Manipulate SERS-Active Substrates by Nanosphere Lithography.

    Zhao, Xiaoyu; Wen, Jiahong; Zhang, Mengning; Wang, Dunhui; Wang, Yaxin; Chen, Lei; Zhang, Yongjun; Yang, Jinghai; Du, Youwei

    2017-03-01

    An easy-handling and low-cost method is utilized to controllably fabricate nanopattern arrays as the surface-enhanced Raman scattering (SERS) active substrates with high density of SERS-active areas (hot spots). A hybrid silver array of nanocaps and nanotriangles are prepared by combining magnetron sputtering and plasma etching. By adjusting the etching time of polystyrene (PS) colloid spheres array in silver nanobowls, the morphology of the arrays can be easily manipulated to control the formation and distribution of hot spots. The experimental results show that the hybrid nanostructural arrays have large enhancement factor, which is estimated to be seven times larger than that in the array of nanocaps and three times larger than that in the array of nanorings and nanoparticles. According to the results of finite-difference time-domain simulation, the excellent SERS performance of this array is ascribed to the high density of hot spots and enhanced electromagnetic field.

  2. LINAC DESIGN FOR AN ARRAY OF SOFT X-RAY FREE ELECTRON LASERS

    Zholents, Alexander A.; Kur, E.; Penn, G.; Qiang, Ji; Venturini, M.; Wells, R. P.

    2008-01-01

    The design of the linac delivering electron bunches into ten independent soft x-ray free electron lasers (FELs) producing light at 1 nm and longer wavelengths is presented. The bunch repetition rate in the linac is 1 MHz and 100 kHz in each of ten FEL beam lines. Various issues regarding machine layout and lattice, bunch compression, collimation, and the beam switch yard are discussed. Particular attention is given to collective effects. A demanding goal is to preserve both a low beam slice emittance and low slice energy spread during acceleration, bunch compression and distribution of the electron bunches into the array of FEL beamlines. Detailed studies of the effect of the electron beam microbunching caused by longitudinal space-charge forces and coherent synchrotron radiation (CSR) have been carried out and their results are presented

  3. Reconfigurable phased antenna array for extending cubesat operations to Ka-band: Design and feasibility

    Buttazzoni, G.; Comisso, M.; Cuttin, A.; Fragiacomo, M.; Vescovo, R.; Vincenti Gatti, R.

    2017-08-01

    Started as educational tools, CubeSats have immediately encountered the favor of the scientific community, subsequently becoming viable platforms for research and commercial applications. To ensure competitive data rates, some pioneers have started to explore the usage of the Ka-band beside the conventional amateur radio frequencies. In this context, this study proposes a phased antenna array design for Ka-band downlink operations consisting of 8×8 circularly polarized subarrays of microstrip patches filling one face of a single CubeSat unit. The conceived structure is developed to support 1.5 GHz bandwidth and dual-task missions, whose feasibility is verified by proper link budgets. The dual-task operations are enabled by a low-complexity phase-only control algorithm that provides pattern reconfigurability in order to satisfy both orbiting and intersatellite missions, while remaining adherent to the cost-effective CubeSat paradigm.

  4. Halbach array-based design and simulation of disc coreless permanen-magnet integrated starter generator

    Li, Y. B.; Yang, Z. X.; Chen, W.; He, Q. Y.

    2017-11-01

    The functional performance, such as magnetic flux leakage, power density and efficiency, is related to the structural characteristics and design technique for the disc permanent magnet synchronous generators (PMSGs). Halbach array theory-based magnetic circuit structure is developed, and Maxwell3D simulation analysis approach of PMSG is proposed in this paper for integrated starter generator (ISG). The magnetization direction of adjacent permanent magnet is organized in difference of 45 degrees for focusing air gap side, and improving the performance of the generator. The magnetic field distribution and functional performance in load and/or unload conditions are simulated by Maxwell3D module. The proposed approach is verified by simulation analysis, the air gap flux density is 0.66T, and the phase voltage curve has the characteristics of a preferable sinusoidal wave and the voltage amplitude 335V can meet the design requirements while the disc coreless PMSG is operating at rated speed. And the developed magnetic circuit structure can be used for engineering design of the disc coreless PMSG to the integrated starter generator.

  5. Design of a Tritium-in-air-monitor using field programmable gate arrays

    McNelles, Phillip; Lu, Lixuan

    2015-01-01

    Field Programmable Gate Arrays (FPGAs) have recently garnered significant interest for certain applications within the nuclear field. Some applications of these devices include Instrumentation and Control (I and C) systems, pulse measurement systems, particle detectors and health physics purposes. In CANada Deuterium Uranium (CANDU) nuclear power plants, the use of heavy water (D2O) as the moderator leads to the increased production of Tritium, which poses a health risk and must be monitored by Tritium-In-Air Monitors (TAMs). Traditional TAMs are mostly designed using microprocessors. More recent studies show that FPGAs could be a potential alternative to implement the electronic logic used in radiation detectors, such as the TAM, more effectively. In this paper, an FPGA-based TAM is designed and constructed in a laboratory setting using an FPGA-based cRIO system. New functionalities, such as the detection of Carbon-14 and the addition of noble gas compensation are incorporated into a new FPGA-based TAM. Additionally, all of the standard functions included in the original microprocessor-based TAM, such as tritium detection, gamma compensation, pump and air flow control, and background and thermal drift corrections were also implemented. The effectiveness of the new design is demonstrated through simulations as well as laboratory testing on the prototype system. (author)

  6. Planar ESPAR Array Design with Nonsymmetrical Pattern by Means of Finite-Element Method, Domain Decomposition, and Spherical Wave Expansion

    Jesús García

    2012-01-01

    Full Text Available The application of a 3D domain decomposition finite-element and spherical mode expansion for the design of planar ESPAR (electronically steerable passive array radiator made with probe-fed circular microstrip patches is presented in this work. A global generalized scattering matrix (GSM in terms of spherical modes is obtained analytically from the GSM of the isolated patches by using rotation and translation properties of spherical waves. The whole behaviour of the array is characterized including all the mutual coupling effects between its elements. This procedure has been firstly validated by analyzing an array of monopoles on a ground plane, and then it has been applied to synthesize a prescribed radiation pattern optimizing the reactive loads connected to the feeding ports of the array of circular patches by means of a genetic algorithm.

  7. Design and operation of a 2-D thin-film semiconductor neutron detector array for use as a beamport monitor

    Unruh, Troy C.; Bellinger, Steven L.; Huddleston, David E.; McNeil, Walter J.; Patterson, Eric; Sobering, Tim J.; McGregor, Douglas S.

    2009-01-01

    Silicon-based diodes coated with a thin film of neutron reactive materials have been shown to produce excellent low-efficiency neutron detectors. This work employs the same technology, but groups 25 equally sized and spaced diodes on a single 29 mm by 29 mm substrate. A 5x5 array was fabricated and coated with a thin film of 6 LiF for use as a low-efficiency neutron beam monitor. The 5x5 neutron detector array is coupled to an array of amplifiers, allowing the response to be interpreted using a LabVIEW FPGA. The 5x5 array has been characterized in a diffracted neutron beam. This work is a part of on-going research to develop various designs of high- and low-efficiency semiconductor neutron detectors.

  8. Design and characterization of a laterally mounted phased-array transducer breast-specific MRgHIFU device with integrated 11-channel receiver array.

    Payne, A; Merrill, R; Minalga, E; Vyas, U; de Bever, J; Todd, N; Hadley, R; Dumont, E; Neumayer, L; Christensen, D; Roemer, R; Parker, D

    2012-03-01

    This work presents the design and preliminary evaluation of a new laterally mounted phased-array MRI-guided high-intensity focused ultrasound (MRgHIFU) system with an integrated 11-channel phased-array radio frequency (RF) coil intended for breast cancer treatment. The design goals for the system included the ability to treat the majority of tumor locations, to increase the MR image's signal-to-noise ratio (SNR) throughout the treatment volume and to provide adequate comfort for the patient. In order to treat the majority of the breast volume, the device was designed such that the treated breast is suspended in a 17-cm diameter treatment cylinder. A laterally shooting 1-MHz, 256-element phased-array ultrasound transducer with flexible positioning is mounted outside the treatment cylinder. This configuration achieves a reduced water volume to minimize RF coil loading effects, to position the coils closer to the breast for increased signal sensitivity, and to reduce the MR image noise associated with using water as the coupling fluid. This design uses an 11-channel phased-array RF coil that is placed on the outer surface of the cylinder surrounding the breast. Mechanical positioning of the transducer and electronic steering of the focal spot enable placement of the ultrasound focus at arbitrary locations throughout the suspended breast. The treatment platform allows the patient to lie prone in a face-down position. The system was tested for comfort with 18 normal volunteers and SNR capabilities in one normal volunteer and for heating accuracy and stability in homogeneous phantom and inhomogeneous ex vivo porcine tissue. There was a 61% increase in mean relative SNR achieved in a homogeneous phantom using the 11-channel RF coil when compared to using only a single-loop coil around the chest wall. The repeatability of the system's energy delivery in a single location was excellent, with less than 3% variability between repeated temperature measurements at the same

  9. Design techniques for a stable operation of cryogenic field-programmable gate arrays

    Homulle, Harald; Visser, Stefan; Patra, Bishnu; Charbon, Edoardo

    2018-01-01

    In this paper, we show how a deep-submicron field-programmable gate array (FPGA) can be operated more stably at extremely low temperatures through special firmware design techniques. Stability at low temperatures is limited through long power supply wires and reduced performance of various printed circuit board components commonly employed at room temperature. Extensive characterization of these components shows that the majority of decoupling capacitor types and voltage regulators are not well behaved at cryogenic temperatures, asking for an ad hoc solution to stabilize the FPGA supply voltage, especially for sensitive applications. Therefore, we have designed a firmware that enforces a constant power consumption, so as to stabilize the supply voltage in the interior of the FPGA. The FPGA is powered with a supply at several meters distance, causing significant resistive voltage drop and thus fluctuations on the local supply voltage. To achieve the stabilization, the variation in digital logic speed, which directly corresponds to changes in supply voltage, is constantly measured and corrected for through a tunable oscillator farm, implemented on the FPGA. The impact of the stabilization technique is demonstrated together with a reconfigurable analog-to-digital converter (ADC), completely implemented in the FPGA fabric and operating at 15 K. The ADC performance can be improved by at most 1.5 bits (effective number of bits) thanks to the more stable supply voltage. The method is versatile and robust, enabling seamless porting to other FPGA families and configurations.

  10. Design and Development of an Array of Dielectric Suspended Membranes for Microhotplate Applications

    Mahanth Prasad

    2014-05-01

    Full Text Available The paper presents the design, fabrication and characterization of an array of suspended dielectric suspended membranes for microhotplate applications. A single cell membrane (100 µm ´ 100 µm made of two different dielectric layers: SiO2 and Si3N4 separately, was designed and simulated using ANSYS 10.0. The simulation of stress generated in different dielectric membranes as a function of temperature is reported. The thickness of both layers was taken as 0.3 µm. The membranes of both SiO2 and Si3N4 dielectrics were fabricated on silicon substrate by bulk micromachining technique using TMAH solution. The buckling of the beam and breakage of membranes made of high-stress Si3N4 film are reported. The simulated results were verified by experiments. The membrane made of SiO2 layer was found to be more suitable in comparison to high-stress Si3N4 layer for microhotplate applications. The present approach provides high yield at low cost for fabrication of microhotplates for gas sensing applications.

  11. Study of the Carrier-Aided Thin Film Electrode Array Design for Cochlear Insertion

    Yuchen Xu

    2018-04-01

    Full Text Available The micro-fabricated thin film electrode array (TFEA has been a promising design for cochlear implants (CIs because of its cost-effectiveness and fabrication precision. The latest polymer-based cochlear TFEAs have faced difficulties for cochlear insertion due to the lack of structural stiffness. To stiffen the TFEA, dissolvable stiffening materials, TFEAs with different structures, and TFEAs with commercial CIs as carriers have been invested. In this work, the concept of enhancing a Parylene TFEA with Kapton tape as a simpler carrier for cochlear insertion has been proved to be feasible. The bending stiffness of the Kapton-aided TFEA was characterized with an analytical model, a finite element model, and a cantilever bending experiment, respectively. While the Kapton tape increased the bending stiffness of the Parylene TFEA by 103 times, the 6-μm-thick TFEA with a similar Young’s modulus, as a polyimide, in turn significantly increased the bending stiffness of the 170-μm-thick Kapton carrier by 60%. This result indicated that even the TFEA is ultra-flexible and that its bending stiffness should not be neglected in the design or selection of its carrier.

  12. Array capabilities and future arrays

    Radford, D.

    1993-01-01

    Early results from the new third-generation instruments GAMMASPHERE and EUROGAM are confirming the expectation that such arrays will have a revolutionary effect on the field of high-spin nuclear structure. When completed, GAMMASHPERE will have a resolving power am order of magnitude greater that of the best second-generation arrays. When combined with other instruments such as particle-detector arrays and fragment mass analysers, the capabilites of the arrays for the study of more exotic nuclei will be further enhanced. In order to better understand the limitations of these instruments, and to design improved future detector systems, it is important to have some intelligible and reliable calculation for the relative resolving power of different instrument designs. The derivation of such a figure of merit will be briefly presented, and the relative sensitivities of arrays currently proposed or under construction presented. The design of TRIGAM, a new third-generation array proposed for Chalk River, will also be discussed. It is instructive to consider how far arrays of Compton-suppressed Ge detectors could be taken. For example, it will be shown that an idealised open-quote perfectclose quotes third-generation array of 1000 detectors has a sensitivity an order of magnitude higher again than that of GAMMASPHERE. Less conventional options for new arrays will also be explored

  13. Millimeter-Wave Microstrip Antenna Array Design and an Adaptive Algorithm for Future 5G Wireless Communication Systems

    Cheng-Nan Hu

    2016-01-01

    Full Text Available This paper presents a high gain millimeter-wave (mmW low-temperature cofired ceramic (LTCC microstrip antenna array with a compact, simple, and low-profile structure. Incorporating minimum mean square error (MMSE adaptive algorithms with the proposed 64-element microstrip antenna array, the numerical investigation reveals substantial improvements in interference reduction. A prototype is presented with a simple design for mass production. As an experiment, HFSS was used to simulate an antenna with a width of 1 mm and a length of 1.23 mm, resonating at 38 GHz. Two identical mmW LTCC microstrip antenna arrays were built for measurement, and the center element was excited. The results demonstrated a return loss better than 15 dB and a peak gain higher than 6.5 dBi at frequencies of interest, which verified the feasibility of the design concept.

  14. The Design, Implementation, and Performance of the Astro-H SXS Calorimeter Array and Anti-Coincidence Detector

    Kilbourne, Caroline A.; Adams, Joseph S.; Brekosky, Regis P.; Chiao, Meng P.; Chervenak, James A.; Eckart, Megan E.; Figueroa-Feliciano, Enectali; Galeazzi, Masimilliano; Grein, Christoph; Jhabvala, Christine A.; hide

    2016-01-01

    The calorimeter array of the JAXA Astro-H (renamed Hitomi) Soft X-ray Spectrometer (SXS) was designed to provide unprecedented spectral resolution of spatially extended cosmic x-ray sources and of all cosmic x-ray sources in the Fe-K band around 6 keV, enabling essential plasma diagnostics. The SXS has a square array of 36 microcalorimeters at the focal plane. These calorimeters consist of ion-implanted silicon thermistors and HgTe thermalizing x-ray absorbers. These devices have demonstrated a resolution of better than 4.5 eV at 6 keV when operated at a heat-sink temperature of 50 mK. We will discuss the basic physical parameters of this array, including the array layout, thermal conductance of the link to the heat sink, resistance function, absorber details, and means of attaching the absorber to the thermistor-bearing element. We will also present the thermal characterization of the whole array, including thermal conductance and crosstalk measurements and the results of pulsing the frame temperature via alpha particles, heat pulses, and the environmental background. A silicon ionization detector is located behind the calorimeter array and serves to reject events due to cosmic rays. We will briefly describe this anti-coincidence detector and its performance.

  15. Assessment of High-Voltage Photovoltaic Technologies for the Design of a Direct Drive Hall Effect Thruster Solar Array

    Mikellides, I. G.; Jongeward, G. A.; Schneider, T.; Carruth, M. R.; Peterson, T.; Kerslake, T. W.; Snyder, D.; Ferguson, D.; Hoskins, A.

    2004-01-01

    A three-year program to develop a Direct Drive Hall-Effect Thruster system (D2HET) begun in 2001 as part of the NASA Advanced Cross-Enterprise Technology Development initiative. The system, which is expected to reduce significantly the power processing, complexity, weight, and cost over conventional low-voltage systems, will employ solar arrays that operate at voltages higher than (or equal to) 300 V. The lessons learned from the development of the technology also promise to become a stepping-stone for the production of the next generation of power systems employing high voltage solar arrays. This paper summarizes the results from experiments conducted mainly at the NASA Marshal Space Flight Center with two main solar array technologies. The experiments focused on electron collection and arcing studies, when the solar cells operated at high voltages. The tests utilized small coupons representative of each solar array technology. A hollow cathode was used to emulate parts of the induced environment on the solar arrays, mostly the low-energy charge-exchange plasma (1012-1013 m-3 and 0.5-1 eV). Results and conclusions from modeling of electron collection are also summarized. The observations from the total effort are used to propose a preliminary, new solar array design for 2 kW and 30-40 kW class, deep space missions that may employ a single or a cluster of Hall- Effect thrusters.

  16. Design of Tunnel Magnetoresistive-Based Circular MFL Sensor Array for the Detection of Flaws in Steel Wire Rope

    Liu Xiucheng

    2016-01-01

    Full Text Available Tunnel magnetoresistive (TMR devices have superior performances in weak magnetic field detection. In this study, TMR devices were first employed to form a circular magnetic flux leakage (MFL sensor for slight wire rope flaw detection. Two versions of this tailor-made circular TMR-based sensor array were presented for the inspection of wire ropes with the diameters of 14 mm and 40 mm, respectively. Helmholtz-like coils or a ferrite magnet-based magnetizer was selected to provide the proper magnetic field, in order to meet the technical requirements of the TMR devices. The coefficient of variance in the flaw detection performance of the sensor array elements was experimentally estimated at 4.05%. Both versions of the MFL sensor array were able to detect multiple single-broken wire flaws in the wire ropes. The accurate axial and circumferential positions of these broken wire flaws were estimated from the MFL scanning image results. In addition, the proposed TMR-based sensor array was applied to detect the MFL signal induced by slight surface wear defects. A mutual correlation analysis method was used to distinguish the signals caused by the lift-off fluctuation from the MFL scanning image results. The MFL sensor arrays presented in this study provide inspiration for the designing of tailor-made TMR-based circular sensor arrays for cylindrical ferromagnetic structural inspections.

  17. Microdeletion and microduplication analysis of chinese conotruncal defects patients with targeted array comparative genomic hybridization.

    Xiaohui Gong

    Full Text Available OBJECTIVE: The current study aimed to develop a reliable targeted array comparative genomic hybridization (aCGH to detect microdeletions and microduplications in congenital conotruncal defects (CTDs, especially on 22q11.2 region, and for some other chromosomal aberrations, such as 5p15-5p, 7q11.23 and 4p16.3. METHODS: Twenty-seven patients with CTDs, including 12 pulmonary atresia (PA, 10 double-outlet right ventricle (DORV, 3 transposition of great arteries (TGA, 1 tetralogy of Fallot (TOF and one ventricular septal defect (VSD, were enrolled in this study and screened for pathogenic copy number variations (CNVs, using Agilent 8 x 15K targeted aCGH. Real-time quantitative polymerase chain reaction (qPCR was performed to test the molecular results of targeted aCGH. RESULTS: Four of 27 patients (14.8% had 22q11.2 CNVs, 1 microdeletion and 3 microduplications. qPCR test confirmed the microdeletion and microduplication detected by the targeted aCGH. CONCLUSION: Chromosomal abnormalities were a well-known cause of multiple congenital anomalies (MCA. This aCGH using arrays with high-density coverage in the targeted regions can detect genomic imbalances including 22q11.2 and other 10 kinds CNVs effectively and quickly. This approach has the potential to be applied to detect aneuploidy and common microdeletion/microduplication syndromes on a single microarray.

  18. Research on analytical model and design formulas of permanent magnetic bearings based on Halbach array with arbitrary segmented magnetized angle

    Wang, Nianxian; Wang, Dongxiong; Chen, Kuisheng; Wu, Huachun

    2016-01-01

    The bearing capacity of permanent magnetic bearings can be improved efficiently by using the Halbach array magnetization. However, the research on analytical model of Halbach array PMBs with arbitrary segmented magnetized angle has not been developed. The application of Halbach array PMBs has been limited by the absence of the analytical model and design formulas. In this research, the Halbach array PMBs with arbitrary segmented magnetized angle has been studied. The magnetization model of bearings is established. The magnetic field distribution model of the permanent magnet array is established by using the scalar magnetic potential model. On the basis of this, the bearing force model and the bearing stiffness model of the PMBs are established based on the virtual displacement method. The influence of the pair of magnetic rings in one cycle and the structure parameters of PMBs on the maximal bearing capacity and support stiffness characteristics are studied. The reference factors for the design process of PMBs have been given. Finally, the theoretical model and the conclusions are verified by the finite element analysis.

  19. Design of a Dry Dilution Refrigerator for MMC Gamma Detector Arrays

    Friedrich, Stephan [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Boyd, Stephen [Univ. of New Mexico, Albuquerque, NM (United States); Cantor, Robin

    2017-04-03

    The goal of this LCP is to develop an ultra-high resolution gamma detector based on magnetic microcalorimeters (MMCs) for accurate non-destructive analysis (NDA) of nuclear materials. For highest energy resolution, we will introduce erbium-doped silver (Ag:Er) as a novel sensor material to replace current Au:Er sensors. The detector sensitivity will be increased by developing arrays of 32 Ag:Er pixels read out by 16 SQUID preamplifiers. MMC detectors require operating temperatures of ~15 mK and thus the use of a dilution refrigerator, and the desire for user-friendly operation without cryogenic liquids requires that this refrigerator use pulse-tube pre-cooling to ~4 K. For long-term reliability, we intend to re-design the heat switch that is needed to apply the magnetizing current to the Ag:Er sensor and that used to fail in earlier designs after months of operation. A cryogenic Compton veto will be installed to reduce the spectral background of the MMC, especially at low energies where ultra-high energy resolution is most important. The goals for FY16 were 1) to purchase a liquid-cryogen-free dilution refrigerator and adapt it for MMC operation, and 2) to fabricate Ag:Er-based MMC γ-detectors with improved performance and optimize their response. This report discusses the design of the instruments, and progress in MMC detector fabrication. Details of the MMC fabrication have been discussed in an April 2016 report to DOE.

  20. Progress on the design of a data push architecture for an array of optimized time tagging pixels

    Shapiro, S.; Cords, D.; Mani, S.; Holbrook, B.; Atlas, E.

    1993-06-01

    A pixel array has been proposed which features a completely data driven architecture. A pixel cell has been designed that has been optimized for this readout. It retains the features of preceding designs which allow low noise operation, time stamping, analog signal processing, XY address recording, ghost elimination and sparse data transmission. The pixel design eliminates a number of problems inherent in previous designs, by the use of sampled data techniques, destructive readout, and current mode output drivers. This architecture and pixel design is directed at applications such as a forward spectrometer at the SSC, an e + e - B factory at SLAC, and fixed target experiments at FNAL

  1. Designing an array for performing Near-field Acoustic Holography with a small number of p-u probes

    Fernandez Comesaña, Daniel; Wen, Junjie; Fernandez Grande, Efren

    2016-01-01

    , such approaches usually require that a large number of transducers is spatially distributed over the area of interest. This paper describes some practical considerations for the design and optimization of a compact sensor array for performing NAH with a small number of sound intensity p-u probes. Two sensor...

  2. Bondwire array modeling for the design of hybrid high power amplifiers above C-band

    Hernández, Carlos Cilla; Jónasson, Sævar Þór; Hanberg, Jesper

    2012-01-01

    This paper presents a bondwire array model obtained using a software based on the finite elements method and validated up to 15 GHz by measurements over a purpose-build array structure. This work addresses the limits of the inductor-based bondwire model when used at frequencies above C-band to si...

  3. An application of CART algorithm in genetics: IGFs and cGH polymorphisms in Japanese quail

    Kaplan, Selçuk

    2017-04-01

    The avian insulin-like growth factor-1 (IGFs) and avian growth hormone (cGH) genes are the most important genes that can affect bird performance traits because of its important function in growth and metabolism. Understanding the molecular genetic basis of variation in growth-related traits is of importance for continued improvement and increased rates of genetic gain. The objective of the present study was to identify polymorphisms of cGH and IGFs genes in Japanese quail using conventional least square method (LSM) and CART algorithm. Therefore, this study was aimed to demonstrate at determining the polymorphisms of two genes related growth characteristics via CART algorithm. A simulated data set was generated to analyze by adhering the results of some poultry genetic studies which it includes live weights at 5 weeks of age, 3 alleles and 6 genotypes of cGH and 2 alleles and 3 genotypes of IGFs. As a result, it has been determined that the CART algorithm has some advantages as for that LSM.

  4. Thin-film sparse boundary array design for passive acoustic mapping during ultrasound therapy.

    Coviello, Christian M; Kozick, Richard J; Hurrell, Andrew; Smith, Penny Probert; Coussios, Constantin-C

    2012-10-01

    A new 2-D hydrophone array for ultrasound therapy monitoring is presented, along with a novel algorithm for passive acoustic mapping using a sparse weighted aperture. The array is constructed using existing polyvinylidene fluoride (PVDF) ultrasound sensor technology, and is utilized for its broadband characteristics and its high receive sensitivity. For most 2-D arrays, high-resolution imagery is desired, which requires a large aperture at the cost of a large number of elements. The proposed array's geometry is sparse, with elements only on the boundary of the rectangular aperture. The missing information from the interior is filled in using linear imaging techniques. After receiving acoustic emissions during ultrasound therapy, this algorithm applies an apodization to the sparse aperture to limit side lobes and then reconstructs acoustic activity with high spatiotemporal resolution. Experiments show verification of the theoretical point spread function, and cavitation maps in agar phantoms correspond closely to predicted areas, showing the validity of the array and methodology.

  5. The high dynamic range pixel array detector (HDR-PAD): Concept and design

    Shanks, Katherine S.; Philipp, Hugh T.; Weiss, Joel T.; Becker, Julian; Tate, Mark W. [Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, NY 14853 (United States); Gruner, Sol M., E-mail: smg26@cornell.edu [Laboratory of Atomic and Solid State Physics, Cornell University, Ithaca, NY 14853 (United States); Cornell High Energy Synchrotron Source (CHESS), Cornell University, Ithaca, NY 14853 (United States)

    2016-07-27

    Experiments at storage ring light sources as well as at next-generation light sources increasingly require detectors capable of high dynamic range operation, combining low-noise detection of single photons with large pixel well depth. XFEL sources in particular provide pulse intensities sufficiently high that a purely photon-counting approach is impractical. The High Dynamic Range Pixel Array Detector (HDR-PAD) project aims to provide a dynamic range extending from single-photon sensitivity to 10{sup 6} photons/pixel in a single XFEL pulse while maintaining the ability to tolerate a sustained flux of 10{sup 11} ph/s/pixel at a storage ring source. Achieving these goals involves the development of fast pixel front-end electronics as well as, in the XFEL case, leveraging the delayed charge collection due to plasma effects in the sensor. A first prototype of essential electronic components of the HDR-PAD readout ASIC, exploring different options for the pixel front-end, has been fabricated. Here, the HDR-PAD concept and preliminary design will be described.

  6. Design and analysis of a dual mode CMOS field programmable analog array

    Cheng Xiaoyan; Yang Haigang; Yin Tao; Wu Qisong; Zhang Hongfeng; Liu Fei

    2014-01-01

    This paper presents a novel field-programmable analog array (FPAA) architecture featuring a dual mode including discrete-time (DT) and continuous-time (CT) operation modes, along with a highly routable connection boxes (CBs) based interconnection lattice. The dual mode circuit for the FPAA is capable of achieving targeted optimal performance in different applications. The architecture utilizes routing switches in a CB not only for the signal interconnection purpose but also for control of the electrical charge transfer required in switched-capacitor circuits. This way, the performance of the circuit in either mode shall not be hampered with adding of programmability. The proposed FPAA is designed and implemented in a 0.18 μm standard CMOS process with a 3.3 V supply voltage. The result from post-layout simulation shows that a maximum bandwidth of 265 MHz through the interconnection network is achieved. The measured results from demonstrated examples show that the maximum signal bandwidth of up to 2 MHz in CT mode is obtained with the spurious free dynamic range of 54 dB, while the signal processing precision in DT mode reaches 96.4%. (semiconductor integrated circuits)

  7. Design and optimization of a high-efficiency array generator in the mid-IR with binary subwavelength grooves.

    Bloom, Guillaume; Larat, Christian; Lallier, Eric; Lee-Bouhours, Mane-Si Laure; Loiseaux, Brigitte; Huignard, Jean-Pierre

    2011-02-10

    We have designed a high-efficiency array generator composed of subwavelength grooves etched in a GaAs substrate for operation at 4.5 μm. The method used combines rigorous coupled wave analysis with an optimization algorithm. The optimized beam splitter has both a high efficiency (∼96%) and a good intensity uniformity (∼0.2%). The fabrication error tolerances are numerically calculated, and it is shown that this subwavelength array generator could be fabricated with current electron beam writers and inductively coupled plasma etching. Finally, we studied the effect of a simple and realistic antireflection coating on the performance of the beam splitter.

  8. CGI: Java software for mapping and visualizing data from array-based comparative genomic hybridization and expression profiling.

    Gu, Joyce Xiuweu-Xu; Wei, Michael Yang; Rao, Pulivarthi H; Lau, Ching C; Behl, Sanjiv; Man, Tsz-Kwong

    2007-10-06

    With the increasing application of various genomic technologies in biomedical research, there is a need to integrate these data to correlate candidate genes/regions that are identified by different genomic platforms. Although there are tools that can analyze data from individual platforms, essential software for integration of genomic data is still lacking. Here, we present a novel Java-based program called CGI (Cytogenetics-Genomics Integrator) that matches the BAC clones from array-based comparative genomic hybridization (aCGH) to genes from RNA expression profiling datasets. The matching is computed via a fast, backend MySQL database containing UCSC Genome Browser annotations. This program also provides an easy-to-use graphical user interface for visualizing and summarizing the correlation of DNA copy number changes and RNA expression patterns from a set of experiments. In addition, CGI uses a Java applet to display the copy number values of a specific BAC clone in aCGH experiments side by side with the expression levels of genes that are mapped back to that BAC clone from the microarray experiments. The CGI program is built on top of extensible, reusable graphic components specifically designed for biologists. It is cross-platform compatible and the source code is freely available under the General Public License.

  9. CGI: Java Software for Mapping and Visualizing Data from Array-based Comparative Genomic Hybridization and Expression Profiling

    Joyce Xiuweu-Xu Gu

    2007-01-01

    Full Text Available With the increasing application of various genomic technologies in biomedical research, there is a need to integrate these data to correlate candidate genes/regions that are identified by different genomic platforms. Although there are tools that can analyze data from individual platforms, essential software for integration of genomic data is still lacking. Here, we present a novel Java-based program called CGI (Cytogenetics-Genomics Integrator that matches the BAC clones from array-based comparative genomic hybridization (aCGH to genes from RNA expression profiling datasets. The matching is computed via a fast, backend MySQL database containing UCSC Genome Browser annotations. This program also provides an easy-to-use graphical user interface for visualizing and summarizing the correlation of DNA copy number changes and RNA expression patterns from a set of experiments. In addition, CGI uses a Java applet to display the copy number values of a specifi c BAC clone in aCGH experiments side by side with the expression levels of genes that are mapped back to that BAC clone from the microarray experiments. The CGI program is built on top of extensible, reusable graphic components specifically designed for biologists. It is cross-platform compatible and the source code is freely available under the General Public License.

  10. Intelligent Design of Metal Oxide Gas Sensor Arrays Using Reciprocal Kernel Support Vector Regression

    Dougherty, Andrew W.

    Metal oxides are a staple of the sensor industry. The combination of their sensitivity to a number of gases, and the electrical nature of their sensing mechanism, make the particularly attractive in solid state devices. The high temperature stability of the ceramic material also make them ideal for detecting combustion byproducts where exhaust temperatures can be high. However, problems do exist with metal oxide sensors. They are not very selective as they all tend to be sensitive to a number of reduction and oxidation reactions on the oxide's surface. This makes sensors with large numbers of sensors interesting to study as a method for introducing orthogonality to the system. Also, the sensors tend to suffer from long term drift for a number of reasons. In this thesis I will develop a system for intelligently modeling metal oxide sensors and determining their suitability for use in large arrays designed to analyze exhaust gas streams. It will introduce prior knowledge of the metal oxide sensors' response mechanisms in order to produce a response function for each sensor from sparse training data. The system will use the same technique to model and remove any long term drift from the sensor response. It will also provide an efficient means for determining the orthogonality of the sensor to determine whether they are useful in gas sensing arrays. The system is based on least squares support vector regression using the reciprocal kernel. The reciprocal kernel is introduced along with a method of optimizing the free parameters of the reciprocal kernel support vector machine. The reciprocal kernel is shown to be simpler and to perform better than an earlier kernel, the modified reciprocal kernel. Least squares support vector regression is chosen as it uses all of the training points and an emphasis was placed throughout this research for extracting the maximum information from very sparse data. The reciprocal kernel is shown to be effective in modeling the sensor

  11. A Design of Wide Band and Wide Beam Cavity-Backed Slot Antenna Array with Slant Polarization

    Huiying Qi

    2016-01-01

    Full Text Available Design of antenna array under the limitation of restricted size is a challenging problem. Cavity-backed slot antenna is widely used because of its advantages of small size, wide band, and wide beam. In this paper, a design of wide band and wide beam cavity-backed slot antenna array with the slant polarization is proposed. To obtain wide band and wide beam with limited size, the inverted microstrip-fed cavity-backed slot antenna (IMF-CBSA is adopted as the element of 1 × 4 antenna array. The slant polarized antennas and their feeding networks are adopted because of their simple structures. The performance of the proposed antenna array is verified by the simulations and experiments. The measured VSWR < 2 bandwidth is 55% at the center frequency 21.8 GHz, and the gain is larger than 12.2 dB. Experimental results demonstrate that the proposed design achieves wide band and beam with the size of 68 mm × 56 mm × 14.5 mm.

  12. A new DOI detector design using discrete crystal array with depth-dependent reflector patterns and single-ended readout

    Lee, Seung-Jae; Lee, Chaeyeong; Kang, Jihoon; Chung, Yong Hyun

    2017-01-01

    We developed a depth of interaction (DOI) positron emission tomography (PET) detector using depth-dependent reflector patterns in a discrete crystal array. Due to the different reflector patterns at depth, light distribution was changed relative to depth. As a preliminary experiment, we measured DOI detector module crystal identification performance. The crystal consisted of a 9×9 array of 2 mmx2 mmx20 mm lutetium-yttrium oxyorthosilicate (LYSO) crystals. The crystal array was optically coupled to a 64-channel position-sensitive photomultiplier tube with a 2 mmx2 mm anode size and an 18.1 mmx18.1 mm effective area. We obtained the flood image with an Anger-type calculation. DOI layers and 9×9 pixels were well distinguished in the obtained images. Preclinical PET scanners based on this detector design offer the prospect of high and uniform spatial resolution.

  13. A new DOI detector design using discrete crystal array with depth-dependent reflector patterns and single-ended readout

    Lee, Seung-Jae; Lee, Chaeyeong [Department of Radiological Science, Yonsei University, Wonju 26493 (Korea, Republic of); Kang, Jihoon, E-mail: ray.jihoon.kang@gmail.com [Department of Biomedical Engineering, Chonnam National University, 50 Daehak-ro, Yeosu, Jeonnam 59626 (Korea, Republic of); Chung, Yong Hyun, E-mail: ychung@yonsei.ac.kr [Department of Radiological Science, Yonsei University, Wonju 26493 (Korea, Republic of)

    2017-01-21

    We developed a depth of interaction (DOI) positron emission tomography (PET) detector using depth-dependent reflector patterns in a discrete crystal array. Due to the different reflector patterns at depth, light distribution was changed relative to depth. As a preliminary experiment, we measured DOI detector module crystal identification performance. The crystal consisted of a 9×9 array of 2 mmx2 mmx20 mm lutetium-yttrium oxyorthosilicate (LYSO) crystals. The crystal array was optically coupled to a 64-channel position-sensitive photomultiplier tube with a 2 mmx2 mm anode size and an 18.1 mmx18.1 mm effective area. We obtained the flood image with an Anger-type calculation. DOI layers and 9×9 pixels were well distinguished in the obtained images. Preclinical PET scanners based on this detector design offer the prospect of high and uniform spatial resolution.

  14. Design, testing, and economics of a 430 W sub p photovoltaic concentrator array for non grid-connected applications

    Maish, A. B.; Rios, M., Jr.; Togami, H.

    A stand-alone 430 W/sub p/ photovoltaic (PV) concentrating system for low power, non grid-connected applications has been designed, fabricated, and tested at Sandia National Laboratories. The array consists of four passively cooled Fresnel lens concentrating modules on a newly developed polar axis tracking structure. Two axis tracking is provided using a self powered clock drive unit mounted on a single post foundation. Test results of tracking accuracy, array output power, parasitic power, performance in winds and array reliability are discussed. using a range of estimated production costs for small production volumes, the life-cycle energy costs have been calculated and compared to the equivalent energy costs of a 3 kW diesel electric generator set and of an equivalent flat panel PV system.

  15. Solar Powered Aircraft, Photovoltaic Array/Battery System Tabletop Demonstration: Design and Operation Manual

    Colozza, Anthony J.; Scheiman, David A.; Bailey, Sheila (Technical Monitor)

    2000-01-01

    A system was constructed to demonstrate the power system operation of a solar powered aircraft. The system consists of a photovoltaic (PV) array, a charge controller, a battery, an electric motor and propeller. The system collects energy from the PV array and either utilizes this energy to operate an electric motor or stores it in a rechargeable battery for future use. The system has a control panel which displays the output of the array and battery as well as the total current going to the electric motor. The control panel also has a means for adjusting the output to the motor to control its speed. The entire system is regulated around 12 VDC.

  16. Array comparative genomic hybridisation analysis of boys with X linked hypopituitarism identifies a 3.9 Mb duplicated critical region at Xq27 containing SOX3.

    Solomon, N.M.; Ross, S.; Morgan, T.; Belsky, J.L.; Hol, F.A.; Karnes, P.; Hopwood, N.J.; Myers, S.E.; Tan, A.; Warne, G.L.; Forrest, S.M.; Thomas, P.Q.

    2004-01-01

    INTRODUCTION: Array comparative genomic hybridisation (array CGH) is a powerful method that detects alteration of gene copy number with greater resolution and efficiency than traditional methods. However, its ability to detect disease causing duplications in constitutional genomic DNA has not been

  17. Chromosomal minimal critical regions in therapy-related leukemia appear different from those of de novo leukemia by high-resolution aCGH.

    Nathalie Itzhar

    Full Text Available Therapy-related acute leukemia (t-AML, is a severe complication of cytotoxic therapy used for primary cancer treatment. The outcome of these patients is poor, compared to people who develop de novo acute leukemia (p-AML. Cytogenetic abnormalities in t-AML are similar to those found in p-AML but present more frequent unfavorable karyotypes depending on the inducting agent. Losses of chromosome 5 or 7 are observed after alkylating agents while balanced translocations are found after topoisomerase II inhibitors. This study compared t-AML to p-AML using high resolution array CGH in order to find copy number abnormalities (CNA at a higher resolution than conventional cytogenetics. More CNAs were observed in 30 t-AML than in 36 p-AML: 104 CNAs were observed with 63 losses and 41 gains (mean number 3.46 per case in t-AML, while in p-AML, 69 CNAs were observed with 32 losses and 37 gains (mean number of 1.9 per case. In primary leukemia with a previously "normal" karyotype, 18% exhibited a previously undetected CNA, whereas in the (few t-AML with a normal karyotype, the rate was 50%. Several minimal critical regions (MCRs were found in t-AML and p-AML. No common MCRs were found in the two groups. In t-AML a 40 kb deleted MCR pointed to RUNX1 on 21q22, a gene coding for a transcription factor implicated in frequent rearrangements in leukemia and in familial thrombocytopenia. In de novo AML, a 1 Mb MCR harboring ERG and ETS2 was observed from patients with complex aCGH profiles. High resolution cytogenomics obtained by aCGH and similar techniques already published allowed us to characterize numerous non random chromosome abnormalities. This work supports the hypothesis that they can be classified into several categories: abnormalities common to all AML; those more frequently found in t-AML and those specifically found in p-AML.

  18. Optimal Design of Low-Density SNP Arrays for Genomic Prediction: Algorithm and Applications.

    Xiao-Lin Wu

    Full Text Available Low-density (LD single nucleotide polymorphism (SNP arrays provide a cost-effective solution for genomic prediction and selection, but algorithms and computational tools are needed for the optimal design of LD SNP chips. A multiple-objective, local optimization (MOLO algorithm was developed for design of optimal LD SNP chips that can be imputed accurately to medium-density (MD or high-density (HD SNP genotypes for genomic prediction. The objective function facilitates maximization of non-gap map length and system information for the SNP chip, and the latter is computed either as locus-averaged (LASE or haplotype-averaged Shannon entropy (HASE and adjusted for uniformity of the SNP distribution. HASE performed better than LASE with ≤1,000 SNPs, but required considerably more computing time. Nevertheless, the differences diminished when >5,000 SNPs were selected. Optimization was accomplished conditionally on the presence of SNPs that were obligated to each chromosome. The frame location of SNPs on a chip can be either uniform (evenly spaced or non-uniform. For the latter design, a tunable empirical Beta distribution was used to guide location distribution of frame SNPs such that both ends of each chromosome were enriched with SNPs. The SNP distribution on each chromosome was finalized through the objective function that was locally and empirically maximized. This MOLO algorithm was capable of selecting a set of approximately evenly-spaced and highly-informative SNPs, which in turn led to increased imputation accuracy compared with selection solely of evenly-spaced SNPs. Imputation accuracy increased with LD chip size, and imputation error rate was extremely low for chips with ≥3,000 SNPs. Assuming that genotyping or imputation error occurs at random, imputation error rate can be viewed as the upper limit for genomic prediction error. Our results show that about 25% of imputation error rate was propagated to genomic prediction in an Angus

  19. Compression and radiation of high-power short rf pulses. II. A novel antenna array design with combined compressor/radiator elements

    Sirenko, Kostyantyn

    2011-01-01

    The paper discusses the radiation of compressed high power short RF pulses using two different types of antennas: (i) A simple monopole antenna and (ii) a novel array design, where each of the elements is constructed by combining a compressor and a radiator. The studies on the monopole antenna demonstrate the possibility of a high power short RF pulse\\'s efficient radiation even using simple antennas. The studies on the novel array design demonstrate that a reduced size array with lower pulse distortion and power decay can be constructed by assembling the array from elements each of which integrates a compressor and a radiator. This design idea can be used with any type of antenna array; in this work it is applied to a phased array.

  20. Design and implementation of Gm-APD array readout integrated circuit for infrared 3D imaging

    Zheng, Li-xia; Yang, Jun-hao; Liu, Zhao; Dong, Huai-peng; Wu, Jin; Sun, Wei-feng

    2013-09-01

    A single-photon detecting array of readout integrated circuit (ROIC) capable of infrared 3D imaging by photon detection and time-of-flight measurement is presented in this paper. The InGaAs avalanche photon diodes (APD) dynamic biased under Geiger operation mode by gate controlled active quenching circuit (AQC) are used here. The time-of-flight is accurately measured by a high accurate time-to-digital converter (TDC) integrated in the ROIC. For 3D imaging, frame rate controlling technique is utilized to the pixel's detection, so that the APD related to each pixel should be controlled by individual AQC to sense and quench the avalanche current, providing a digital CMOS-compatible voltage pulse. After each first sense, the detector is reset to wait for next frame operation. We employ counters of a two-segmental coarse-fine architecture, where the coarse conversion is achieved by a 10-bit pseudo-random linear feedback shift register (LFSR) in each pixel and a 3-bit fine conversion is realized by a ring delay line shared by all pixels. The reference clock driving the LFSR counter can be generated within the ring delay line Oscillator or provided by an external clock source. The circuit is designed and implemented by CSMC 0.5μm standard CMOS technology and the total chip area is around 2mm×2mm for 8×8 format ROIC with 150μm pixel pitch. The simulation results indicate that the relative time resolution of the proposed ROIC can achieve less than 1ns, and the preliminary test results show that the circuit function is correct.

  1. Electrical crosstalk in front-illuminated photodiode array with different guard ring designs for medical CT applications

    Ji Fan; Juntunen, Mikko; Hietanen, Iiro

    2009-01-01

    This paper presents electrical crosstalk studies on front-illuminated photodiode arrays for medical computed tomography (CT) applications. Crosstalk is an important factor to the system noise and image quality. The electrical crosstalk depends on silicon substrate properties and photodiode structures. The photodiode samples employed in this paper are planar processed on high-resistivity n-type silicon substrate, resulting in a p+/n-/n+ diode structure. Two types of guard ring structures are designed and applied to the same geometry of two-dimensional photodiode arrays. One structure is an n guard ring in the gap area between pixels, and the other structure is an additional p+ guard ring around each pixel together with the n guard ring. A 10 μm light spot with wavelength of 525 nm is used to scan across the surface of the photodiode array in the electrical crosstalk measurements. The electrical currents of two neighbor pixels are measured and the results are compared between two guard ring designs. The design with the p+ guard ring structure gives better electrical crosstalk suppression. Moreover, the measurement results show much smaller influence on surrounding pixels with the p+ guard ring structure in the case of disconnected pixel. Besides the electrical crosstalk, the light sensitivity within the gap area is also discussed between two guard ring designs.

  2. Design a freeform microlens array module for any arbitrary-shape collimated beam shaping and color mixing

    Chen, Enguo; Wu, Rengmao; Guo, Tailiang

    2014-06-01

    Collimated beam shaping with freeform surface usually employs a predefined mapping to tailor one or multiple freeform surfaces. Limitation on those designs is that the source, the freeform optics and the target are in fixed one-to-one correspondence with each other. To overcome this drawback, this paper presents a kind of freeform microlens array module integrated with an ultra-thin freeform microlens array and a condenser lens to reshape any arbitrary-shape collimated beam into a prescribed uniform rectangular illumination and achieve color mixing. The design theory is explicitly given, and some key issues are addressed. Several different application examples are given, and the target is obtained with high uniformity and energy efficiency. This freeform microlens array module, which shows better flexibility and practicality than the regular designs, can be used not only to reshape any arbitrary-shape collimated beam (or a collimated beam integrated with several sub-collimated beams), but also most importantly to achieve color mixing. With excellent optical performance and ultra-compact volume, this optical module together with the design theory can be further introduced into other applications and will have a huge market potential in the near future.

  3. A review of the design and clinical evaluation of the ShefStim array-based functional electrical stimulation system.

    Kenney, Laurence P; Heller, Ben W; Barker, Anthony T; Reeves, Mark L; Healey, Jamie; Good, Timothy R; Cooper, Glen; Sha, Ning; Prenton, Sarah; Liu, Anmin; Howard, David

    2016-11-01

    Functional electrical stimulation has been shown to be a safe and effective means of correcting foot drop of central neurological origin. Current surface-based devices typically consist of a single channel stimulator, a sensor for determining gait phase and a cuff, within which is housed the anode and cathode. The cuff-mounted electrode design reduces the likelihood of large errors in electrode placement, but the user is still fully responsible for selecting the correct stimulation level each time the system is donned. Researchers have investigated different approaches to automating aspects of setup and/or use, including recent promising work based on iterative learning techniques. This paper reports on the design and clinical evaluation of an electrode array-based FES system for the correction of drop foot, ShefStim. The paper reviews the design process from proof of concept lab-based study, through modelling of the array geometry and interface layer to array search algorithm development. Finally, the paper summarises two clinical studies involving patients with drop foot. The results suggest that the ShefStim system with automated setup produces results which are comparable with clinician setup of conventional systems. Further, the final study demonstrated that patients can use the system without clinical supervision. When used unsupervised, setup time was 14min (9min for automated search plus 5min for donning the equipment), although this figure could be reduced significantly with relatively minor changes to the design. Copyright © 2016 IPEM. Published by Elsevier Ltd. All rights reserved.

  4. Design of optical element combining Fresnel lens with microlens array for uniform light-emitting diode lighting.

    Wang, Guangzhen; Wang, Lili; Li, Fuli; Kong, Depeng

    2012-09-01

    One kind of optical element combining Fresnel lens with microlens array is designed simply for LED lighting based on geometrical optics and nonimaging optics. This design method imposes no restriction on the source intensity pattern. The designed element has compact construction and can produce multiple shapes of illumination distribution. Taking square lighting as an example, tolerance analysis is carried out to determine tolerance limits for applying the element in the assembly process. This element can produce on-axis lighting and off-axis lighting.

  5. Interferometry with flexible point source array for measuring complex freeform surface and its design algorithm

    Li, Jia; Shen, Hua; Zhu, Rihong; Gao, Jinming; Sun, Yue; Wang, Jinsong; Li, Bo

    2018-06-01

    The precision of the measurements of aspheric and freeform surfaces remains the primary factor restrict their manufacture and application. One effective means of measuring such surfaces involves using reference or probe beams with angle modulation, such as tilted-wave-interferometer (TWI). It is necessary to improve the measurement efficiency by obtaining the optimum point source array for different pieces before TWI measurements. For purpose of forming a point source array based on the gradients of different surfaces under test, we established a mathematical model describing the relationship between the point source array and the test surface. However, the optimal point sources are irregularly distributed. In order to achieve a flexible point source array according to the gradient of test surface, a novel interference setup using fiber array is proposed in which every point source can be independently controlled on and off. Simulations and the actual measurement examples of two different surfaces are given in this paper to verify the mathematical model. Finally, we performed an experiment of testing an off-axis ellipsoidal surface that proved the validity of the proposed interference system.

  6. Design, fabrication, and calibration of a cryogenic search-coil array for harmonic analysis of quadrupole magnets

    Green, M.I.; Barale, P.J.; Hassenzahl, W.V.; Nelson, D.H.; O'Neill, J.W.; Schafer, R.V.; Taylor, C.E.

    1987-09-01

    A cryogenic search-coil array has been fabricated at LBL for harmonic error analysis of SSC model quadrupoles. It consists of three triplets of coils; the center-coil triplet is 10 cm long, and the end coil triplets are 70 cm long. Design objectives are a high bucking ratio for the dipole and quadrupole signals and utility at cryogenic operating currents (∼6 kA) with sufficient sensitivity for use at room-temperature currents (∼10 A). the design and fabrication are described. Individual coils are mechanically measured to +-5 μm, and their magnetic areas measured to 0.05%. A computer program has been developed to predict the quadrupole and dipole bucking ratios from the mechanical and magnetic measurements. The calibration procedure and accuracy of the array are specified. Results of measurements of SSC model quadrupoles are presented. 1 ref., 4 figs

  7. Design of a Large-scale Three-dimensional Flexible Arrayed Tactile Sensor

    Junxiang Ding

    2011-01-01

    Full Text Available This paper proposes a new type of large-scale three-dimensional flexible arrayed tactile sensor based on conductive rubber. It can be used to detect three-dimensional force information on the continuous surface of the sensor, which realizes a true skin type tactile sensor. The widely used method of liquid rubber injection molding (LIMS method is used for "the overall injection molding" sample preparation. The structure details of staggered nodes and a new decoupling algorithm of force analysis are given. Simulation results show that the sensor based on this structure can achieve flexible measurement of large-scale 3-D tactile sensor arrays.

  8. Optimal design of a double-sided linear motor with a multi-segmented trapezoidal magnet array for a high precision positioning system

    Lee, Moon G.; Gweon, Dae-Gab

    2004-01-01

    A comparative analysis is performed for linear motors adopting conventional and multi-segmented trapezoidal (MST) magnet arrays, respectively, for a high-precision positioning system. The proposed MST magnet array is a modified version of a Halbach magnet array. The MST array has trapezoidal magnets with variable shape and dimensions while the Halbach magnet array generally has a rectangular magnet with identical dimensions. We propose a new model that can describe the magnetic field resulting from the complex-shaped magnets. The model can be applied to both MST and conventional magnet arrays. Using the model, a design optimization of the two types of linear motors is performed and compared. The magnet array with trapezoidal magnets can produce more force than one with rectangular magnets when they are arrayed in a linear motor where there is a yoke with high permeability. After the optimization and comparison, we conclude that the linear motor with the MST magnet array can generate more actuating force per volume than the motor with the conventional array. In order to satisfy the requirements of next generation systems such as high resolution, high speed, and long stroke, the use of a linear motor with a MST array as an actuator in a high precision positioning system is recommended from the results obtained here

  9. Planar array stack design aided by rapid prototyping in development of air-breathing PEMFC

    Chen, Chen-Yu; Lai, Wei-Hsiang; Weng, Biing-Jyh; Chuang, Huey-Jan; Hsieh, Ching-Yuan; Kung, Chien-Chih

    The polymer electrolyte membrane fuel cell (PEMFC) is one of the most important research topics in the new and clean energy area. The middle or high power PEMFCs can be applied to the transportation or the distributed power system. But for the small power application, it is needed to match the power requirement of the product generally. On the other hand, the direct methanol fuel cell (DMFC) is one of the most common type that researchers are interested in, but recently the miniature or the micro-PEMFCs attract more attention due to their advantages of high open circuit voltage and high power density. The objective of this study is to develop a new air-breathing planar array fuel cell stacked from 10 cells made by rapid prototyping technology which has potential for fast commercial design, low cost manufacturing, and even without converters/inverters for the system. In this paper, the main material of flow field plates is acrylonitrile-butadiene-styrene (ABS) which allows the fuel cell be mass-manufactured by plastic injection molding technology. The rapid prototyping technology is applied to construct the prototype and verify the practicability of the proposed stack design. A 10-cell air-breathing miniature PEMFC stack with a volume of 6 cm × 6 cm × 0.9 cm is developed and tested. Its segmented membrane electrode assembly (MEA) is designed with the active surface area of 1.3 cm × 1.3 cm in each individual MEA. The platinum loading at anode and cathode are 0.2 mg cm -2 and 0.4 mg cm -2, respectively. Results show that the peak power densities of the parallel connected and serial connected stack are 99 mW cm -2 at 0.425 V and 92 mW cm -2 at 4.25 V, respectively under the conditions of 70 °C relative saturated humidity (i.e., dew point temperature), ambient temperature and free convection air. Besides, the stack performance is increased under forced convection. If the cell surface air is blown by an electric fan, the peak power densities of parallel connected and

  10. Conceptual design of the early implementation of the NEutron Detector Array (NEDA) with AGATA

    Hüyük, Tayfun; Di Nitto, Antonio; Jaworski, Grzegorz; Gadea, Andrés; Javier Valiente-Dobón, José; Nyberg, Johan; Palacz, Marcin; Söderström, Pär-Anders; Jose Aliaga-Varea, Ramon; de Angelis, Giacomo; Ataç, Ayşe; Collado, Javier; Domingo-Pardo, Cesar; Egea, Francisco Javier; Erduran, Nizamettin; Ertürk, Sefa; de France, Gilles; Gadea, Rafael; González, Vicente; Herrero-Bosch, Vicente; Kaşkaş, Ayşe; Modamio, Victor; Moszynski, Marek; Sanchis, Enrique; Triossi, Andrea; Wadsworth, Robert

    2016-03-01

    The NEutron Detector Array (NEDA) project aims at the construction of a new high-efficiency compact neutron detector array to be coupled with large γ-ray arrays such as AGATA. The application of NEDA ranges from its use as selective neutron multiplicity filter for fusion-evaporation reaction to a large solid angle neutron tagging device. In the present work, possible configurations for the NEDA coupled with the Neutron Wall for the early implementation with AGATA has been simulated, using Monte Carlo techniques, in order to evaluate their performance figures. The goal of this early NEDA implementation is to improve, with respect to previous instruments, efficiency and capability to select multiplicity for fusion-evaporation reaction channels in which 1, 2 or 3 neutrons are emitted. Each NEDA detector unit has the shape of a regular hexagonal prism with a volume of about 3.23l and it is filled with the EJ301 liquid scintillator, that presents good neutron- γ discrimination properties. The simulations have been performed using a fusion-evaporation event generator that has been validated with a set of experimental data obtained in the 58Ni + 56Fe reaction measured with the Neutron Wall detector array.

  11. Array Design: Literature Survey For A High-Resolution Imaging Sonal System. Part 1

    1993-12-01

    radioastronomy or microwave arrays, or it may be elastic or acoustic (same as elastic but .-estricted to fields specified by a scalar quantity). There is...these are direction cosines (or sines) (see Ziomek 1985, or a radioastronomy book such as Perley et al. 1989). This 0 is because the Fourier

  12. Conceptual design of the early implementation of the NEutron Detector Array (NEDA) with AGATA

    Hueyuek, Tayfun; Gadea, Andres; Domingo-Pardo, Cesar [Universidad de Valencia, Instituto de Fisica Corpuscular, CSIC, Paterna (Valencia) (Spain); Di Nitto, Antonio [Istituto Nazionale di Fisica Nucleare, Napoli (Italy); Johannes Gutenberg-Universitaet Mainz, Mainz (Germany); Jaworski, Grzegorz; Javier Valiente-Dobon, Jose; De Angelis, Giacomo; Modamio, Victor; Triossi, Andrea [Laboratori Nazionali di Legnaro, Istituto Nazionale di Fisica Nucleare, Legnaro (Italy); Nyberg, Johan [Uppsala University, Department of Physics and Astronomy, Uppsala (Sweden); Palacz, Marcin [University of Warsaw, Heavy Ion Laboratory, Warsaw (Poland); Soederstroem, Paer-Anders [RIKEN Nishina Center, Saitama (Japan); Aliaga-Varea, Ramon Jose [Universidad de Valencia, Instituto de Fisica Corpuscular, CSIC, Paterna (Valencia) (Spain); Universidad Politecnica de Valencia, I3M, Valencia (Spain); Atac, Ayse [Ankara University, Department of Physics, Faculty of Sciences, Ankara (Turkey); The Royal Institute of Technology, Stockholm (Sweden); Collado, Javier; Egea, Francisco Javier; Gonzalez, Vicente; Sanchis, Enrique [University of Valencia, Department of Electronic Engineering, Burjassot (Valencia) (Spain); Erduran, Nizamettin [Istanbul Sabahattin Zaim University, Faculty of Engineering and Natural Sciences, Istanbul (Turkey); Ertuerk, Sefa [University of Nigde, Department of Physics, Faculty of Science and Arts, Nigde (Turkey); France, Gilles de [CNRS/IN2P3, GANIL, CEA/DSAM, Caen (France); Gadea, Rafael; Herrero-Bosch, Vicente [Universidad Politecnica de Valencia, I3M, Valencia (Spain); Kaskas, Ayse [Ankara University, Department of Physics, Faculty of Sciences, Ankara (Turkey); Moszynski, Marek [National Centre for Nuclear Research, Otwock-Swierk (Poland); Wadsworth, Robert [University of York, Department of Physics, York (United Kingdom)

    2016-03-15

    The NEutron Detector Array (NEDA) project aims at the construction of a new high-efficiency compact neutron detector array to be coupled with large γ -ray arrays such as AGATA. The application of NEDA ranges from its use as selective neutron multiplicity filter for fusion-evaporation reaction to a large solid angle neutron tagging device. In the present work, possible configurations for the NEDA coupled with the Neutron Wall for the early implementation with AGATA has been simulated, using Monte Carlo techniques, in order to evaluate their performance figures. The goal of this early NEDA implementation is to improve, with respect to previous instruments, efficiency and capability to select multiplicity for fusion-evaporation reaction channels in which 1, 2 or 3 neutrons are emitted. Each NEDA detector unit has the shape of a regular hexagonal prism with a volume of about 3.23l and it is filled with the EJ301 liquid scintillator, that presents good neutron- γ discrimination properties. The simulations have been performed using a fusion-evaporation event generator that has been validated with a set of experimental data obtained in the {sup 58}Ni + {sup 56}Fe reaction measured with the Neutron Wall detector array. (orig.)

  13. Concept, Design, and Prototyping of XSAS: A High Power Extendable Solar Array for CubeSat Applications

    Senatore, Patrick; Klesh, Andrew; Zurbuchen, Thomas H.; McKague, Darren; Cutler, James

    2010-01-01

    CubeSats have proven themselves as a reliable and cost-effective method to perform experiments in space, but they are highly constrained by their specifications and size. One such constraint is the average continuous power, about 5 W, which is available to the typical CubeSat. To improve this constraint, we have developed the eXtendable Solar Array System (XSAS), a deployable solar array prototype in a CubeSat package, which can provide an average 23 W of continuous power. The prototype served as a technology demonstrator for the high risk mechanisms needed to release, deploy, and control the solar array. Aside from this drastic power increase, it is in the integration of each mechanism, their application within the small CubeSat form-factor, and the inherent passive control benefit of the deployed geometry that make XSAS a novel design. In this paper, we discuss the requirements and design process for the XSAS system and mechanical prototype, and provide qualitative and quantitative results from numerical simulations and prototype tests. We also discuss future work, including an upcoming NASA zero-gravity flight campaign, to further improve on XSAS and prepare it for future launch opportunities.

  14. Design of photonic phased array switches using nano electromechanical systems on silicon-on-insulator integration platform

    Hussein, Ali Abdulsattar

    This thesis presents an introduction to the design and simulation of a novel class of integrated photonic phased array switch elements. The main objective is to use nano-electromechanical (NEMS) based phase shifters of cascaded under-etched slot nanowires that are compact in size and require a small amount of power to operate them. The structure of the switch elements is organized such that it brings the phase shifting elements to the exterior sides of the photonic circuits. The transition slot couplers, used to interconnect the phase shifters, are designed to enable biasing one of the silicon beams of each phase shifter from an electrode located at the side of the phase shifter. The other silicon beam of each phase shifter is biased through the rest of the silicon structure of the switch element, which is taken as a ground. Phased array switch elements ranging from 2x2 up to 8x8 multiple-inputs/multiple-outputs (MIMO) are conveniently designed within reasonable footprints native to the current fabrication technologies. Chapter one presents the general layout of the various designs of the switch elements and demonstrates their novel features. This demonstration will show how waveguide disturbances in the interconnecting network from conventional switch elements can be avoided by adopting an innovative design. Some possible applications for the designed switch elements of different sizes and topologies are indicated throughout the chapter. Chapter two presents the design of the multimode interference (MMI) couplers used in the switch elements as splitters, combiners and waveguide crossovers. Simulation data and design methodologies for the multimode couplers of interest are detailed in this chapter. Chapter three presents the design and analysis of the NEMS-operated phase shifters. Both simulations and numerical analysis are utilized in the design of a 0°-180° capable NEMS-operated phase shifter. Additionally, the response of some of the designed photonic phased

  15. Genomic imbalances in 5918 malignant epithelial tumors: an explorative meta-analysis of chromosomal CGH data

    Baudis, Michael

    2007-01-01

    Chromosomal abnormalities have been associated with most human malignancies, with gains and losses on some genomic regions associated with particular entities. Of the 15429 cases collected for the Progenetix molecular-cytogenetic database, 5918 malignant epithelial neoplasias analyzed by chromosomal Comparative Genomic Hybridization (CGH) were selected for further evaluation. For the 22 clinico-pathological entities with more than 50 cases, summary profiles for genomic imbalances were generated from case specific data and analyzed. With large variation in overall genomic instability, recurring genomic gains and losses were prominent. Most entities showed frequent gains involving 8q2, while gains on 20q, 1q, 3q, 5p, 7q and 17q were frequent in different entities. Loss 'hot spots' included 3p, 4q, 13q, 17p and 18q among others. Related average imbalance patterns were found for clinically distinct entities, e.g. hepatocellular carcinomas (ca.) and ductal breast ca., as well as for histologically related entities (squamous cell ca. of different sites). Although considerable case-by-case variation of genomic profiles can be found by CGH in epithelial malignancies, a limited set of variously combined chromosomal imbalances may be typical for carcinogenesis. Focus on the respective regions should aid in target gene detection and pathway deduction

  16. A study of glasses-type color CGH using a color filter considering reduction of blurring

    Iwami, Saki; Sakamoto, Yuji

    2009-02-01

    We have developed a glasses-type color computer generated hologram (CGH) by using a color filter. The proposed glasses consist of two "lenses" made of overlapping holograms and color filters. The holograms, which are calculated to reconstruct images in each primary color, are divided to small areas, which we called cells, and superimposed on one hologram. In the same way, colors of the filter correspond to the hologram cells. We can configure it very simply without a complex optical system, and the configuration yields a small and light weight system suitable for glasses. When the cell is small enough, the colors are mixed and reconstructed color images are observed. In addition, color expression of reconstruction images improves, too. However, using small cells blurrs reconstructed images because of the following reasons: (1) interference between cells because of the correlation with the cells, and (2) reduction of resolution caused by the size of the cell hologram. We are investigating in order to make a hologram that has high resolution reconstructed color images without ghost images. In this paper, we discuss (1) the details of the proposed glasses-type color CGH, (2) appropriate cell size for an eye system, (3) effects of cell shape on the reconstructed images, and (4) a new method to reduce the blurring of the images.

  17. Identification of chromosome aberrations in sporadic microsatellite stable and unstable colorectal cancers using array comparative genomic hybridization

    Jensen, Thomas Dyrsø; Li, Jian; Wang, Kai

    2011-01-01

    cancers constitute approximately 85% of sporadic cases, whereas microsatellite unstable (MSI) cases constitute the remaining 15%. In this study, we used array comparative genomic hybridization (aCGH) to identify genomic hotspot regions that harbor recurrent copy number changes. The study material...

  18. High resolution array-based comparative genomic hybridisation of medulloblastomas and supra-tentorial primitive neuroectodermal tumours

    McCabe, Martin Gerard; Ichimura, Koichi; Liu, Lu; Plant, Karen; Bäcklund, L Magnus; Pearson, Danita M; Collins, Vincent Peter

    2010-01-01

    Medulloblastomas and supratentorial primitive neuroectodermal tumours are aggressive childhood tumours. We report our findings using array comparative genomic hybridisation (CGH) on a whole-genome BAC/PAC/cosmid array with a median clone separation of 0.97Mb to study 34 medulloblastomas and 7 supratentorial primitive neuroectodermal tumours. Array CGH allowed identification and mapping of numerous novel small regions of copy number change to genomic sequence, in addition to the large regions already known from previous studies. Novel amplifications were identified, some encompassing oncogenes, MYCL1, PDGFRA, KIT and MYB, not previously reported to show amplification in these tumours. In addition, one supratentorial primitive neuroectodermal tumour had lost both copies of the tumour suppressor genes CDKN2A & CDKN2B. Ten medulloblastomas had findings suggestive of isochromosome 17q. In contrast to previous reports using conventional CGH, array CGH identified three distinct breakpoints in these cases: Ch 17: 17940393-19251679 (17p11.2, n=6), Ch 17: 20111990-23308272 (17p11.2-17q11.2, n=4) and Ch 17: 38425359-39091575 (17q21.31, n=1). Significant differences were found in the patterns of copy number change between medulloblastomas and supratentorial primitive neuroectodermal tumours, providing further evidence that these tumours are genetically distinct despite their morphological and behavioural similarities. PMID:16783165

  19. Design, development and evaluation of a resistor-based multiplexing circuit for a 20×20 SiPM array

    Wang, Zhonghai; Sun, Xishan; Lou, Kai; Meier, Joseph; Zhou, Rong; Yang, Chaowen; Zhu, Xiaorong; Shao, Yiping

    2016-01-01

    One technical challenge in developing a large-size scintillator detector with multiple Silicon Photomultiplier (SiPM) arrays is to read out a large number of detector output channels. To achieve this, different signal multiplexing circuits have been studied and applied with different performances and cost-effective tradeoffs. Resistor-based multiplexing circuits exhibit simplicity and signal integrity, but also present the disadvantage of timing shift among different channels. In this study, a resistor-based multiplexing circuit for a large-sized SiPM array readout was developed and evaluated by simulation and experimental studies. Similarly to a multiplexing circuit used for multi-anode PMT, grounding and branching resistors were connected to each SiPM output channel. The grounding resistor was used to simultaneously reduce the signal crosstalk among different channels and to improve timing performance. Both grounding and branching resistor values were optimized to maintain a balanced performance of the event energy, timing, and positioning. A multiplexing circuit was implemented on a compact PCB and applied for a flat-panel detector which consisted of a 32×32 LYSO scintillator crystals optically coupled to 5×5 SiPM arrays for a total 20×20 output channels. Test results showed excellent crystal identification for all 1024 LYSO crystals (each with 2×2×30 mm"3 size) with "2"2Na flood-source irradiation. The measured peak-to-valley ratio from typical crystal map profile is around 3:1 to 6.6:1, an average single crystal energy resolution of about 17.3%, and an average single crystal timing resolution of about 2 ns. Timing shift among different crystals, as reported in some other resistor-based multiplexing circuit designs, was not observed. In summary, we have designed and implemented a practical resistor-based multiplexing circuit that can be readily applied for reading out a large SiPM array with good detector performance.

  20. Design, development and evaluation of a resistor-based multiplexing circuit for a 20×20 SiPM array

    Wang, Zhonghai [College of Physical Science and Technology, Key Laboratory of Radiation Physics and Technology, Ministry of Education, Sichuan University, Chengdu (China); Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, Tx (United States); Sun, Xishan [Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, Tx (United States); Lou, Kai [Department of Electrical and Computer Engineering, Rice University, Houston, Tx (United States); Meier, Joseph [Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, Houston, Tx (United States); Zhou, Rong; Yang, Chaowen [College of Physical Science and Technology, Key Laboratory of Radiation Physics and Technology, Ministry of Education, Sichuan University, Chengdu (China); Zhu, Xiaorong [Department of Radiation Physics, The University of Texas MD Anderson Cancer Center, Houston, Tx (United States); Shao, Yiping [Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, Tx (United States)

    2016-04-21

    One technical challenge in developing a large-size scintillator detector with multiple Silicon Photomultiplier (SiPM) arrays is to read out a large number of detector output channels. To achieve this, different signal multiplexing circuits have been studied and applied with different performances and cost-effective tradeoffs. Resistor-based multiplexing circuits exhibit simplicity and signal integrity, but also present the disadvantage of timing shift among different channels. In this study, a resistor-based multiplexing circuit for a large-sized SiPM array readout was developed and evaluated by simulation and experimental studies. Similarly to a multiplexing circuit used for multi-anode PMT, grounding and branching resistors were connected to each SiPM output channel. The grounding resistor was used to simultaneously reduce the signal crosstalk among different channels and to improve timing performance. Both grounding and branching resistor values were optimized to maintain a balanced performance of the event energy, timing, and positioning. A multiplexing circuit was implemented on a compact PCB and applied for a flat-panel detector which consisted of a 32×32 LYSO scintillator crystals optically coupled to 5×5 SiPM arrays for a total 20×20 output channels. Test results showed excellent crystal identification for all 1024 LYSO crystals (each with 2×2×30 mm{sup 3} size) with {sup 22}Na flood-source irradiation. The measured peak-to-valley ratio from typical crystal map profile is around 3:1 to 6.6:1, an average single crystal energy resolution of about 17.3%, and an average single crystal timing resolution of about 2 ns. Timing shift among different crystals, as reported in some other resistor-based multiplexing circuit designs, was not observed. In summary, we have designed and implemented a practical resistor-based multiplexing circuit that can be readily applied for reading out a large SiPM array with good detector performance.

  1. The Design and Analysis of Split Row-Column Addressing Array for 2-D Transducer

    Xu Li

    2016-09-01

    Full Text Available For 3-D ultrasound imaging, the row-column addressing (RCA with 2N connections for an N × N 2-D array makes the fabrication and interconnection simpler than the fully addressing with N2 connections. However, RCA degrades the image quality because of defocusing in signal channel direction in the transmit event. To solve this problem, a split row-column addressing scheme (SRCA is proposed in this paper. Rather than connecting all the elements in the signal channel direction together, this scheme divides the elements in the signal channel direction into several disconnected blocks, thus enables focusing beam access in both signal channel and switch channel directions. Selecting an appropriate split scheme is the key for SRCA to maintaining a reasonable tradeoff between the image quality and the number of connections. Various split schemes for a 32 × 32 array are fully investigated with point spread function (PSF analysis and imaging simulation. The result shows the split scheme with five blocks (4, 6, 12, 6, and 4 elements of each block can provide similar image quality to fully addressing. The splitting schemes for different array sizes from 16 × 16 to 96 × 96 are also discussed.

  2. Array design considerations for exploitation of stable weakly dispersive modal pulses in the deep ocean

    Udovydchenkov, Ilya A.

    2017-07-01

    Modal pulses are broadband contributions to an acoustic wave field with fixed mode number. Stable weakly dispersive modal pulses (SWDMPs) are special modal pulses that are characterized by weak dispersion and weak scattering-induced broadening and are thus suitable for communications applications. This paper investigates, using numerical simulations, receiver array requirements for recovering information carried by SWDMPs under various signal-to-noise ratio conditions without performing channel equalization. Two groups of weakly dispersive modal pulses are common in typical mid-latitude deep ocean environments: the lowest order modes (typically modes 1-3 at 75 Hz), and intermediate order modes whose waveguide invariant is near-zero (often around mode 20 at 75 Hz). Information loss is quantified by the bit error rate (BER) of a recovered binary phase-coded signal. With fixed receiver depths, low BERs (less than 1%) are achieved at ranges up to 400 km with three hydrophones for mode 1 with 90% probability and with 34 hydrophones for mode 20 with 80% probability. With optimal receiver depths, depending on propagation range, only a few, sometimes only two, hydrophones are often sufficient for low BERs, even with intermediate mode numbers. Full modal resolution is unnecessary to achieve low BERs. Thus, a flexible receiver array of autonomous vehicles can outperform a cabled array.

  3. Design of S-Band Phased Array Antenna with High Isolation Using Broadside Coupled Split Ring Resonator

    Sungyoun Hwang

    2018-04-01

    Full Text Available In this paper, a method of designing a Vivaldi type phased array antenna (PAA which operates at S-band (2.8–3.3 GHz is presented. The presented antenna uses broadside coupled split ring resonators (BC-SRRs for high isolation, wide field of view, and good active S-parameter characteristics. As an example, we design a 1 × 8 array antenna with various BC-SRR structures using theory and EM simulations. The antenna is fabricated and measured to verify the design. With the BC-SRR implemented between the two radiating elements, the isolation is shown to be enhanced by 6 dB, up to 23 dB. The scan angle is shown to be within ±53° based on a −10 dB active reflection coefficient. The operation of the scan angle is possible within ±60° with a little larger reflection coefficient (−7 dB to −8 dB. The proposed design with BC-SRRs is expected to be useful for PAA applications.

  4. Design, development, manufacture, testing, and delivery of devices for connection of solar cell panel circuitry to flat conductor cable solar cell array harness

    Dillard, P. A.; Waddington, D.

    1971-01-01

    The technology status and problem areas which exist for the application of flat conductor cabling to solar cell arrays are summarized. Details covering the design, connector manufacture, and prototype test results are also summarized.

  5. Design of a Weighted-Rotor Energy Harvester Based on Dynamic Analysis and Optimization of Circular Halbach Array Magnetic Disk

    Yu-Jen Wang

    2015-03-01

    Full Text Available This paper proposes the design of a weighted-rotor energy harvester (WREH in which the oscillation is caused by the periodic change of the tangential component of gravity, to harvest kinetic energy from a rotating wheel. When a WREH is designed with a suitable characteristic length, the rotor’s natural frequency changes according to the wheel rotation speed and the rotor oscillates at a wide angle and high angular velocity to generate a large amount of power. The magnetic disk is designed according to an optimized circular Halbach array. The optimized circular Halbach array magnetic disk provides the largest induced EMF for different sector-angle ratios for the same magnetic disk volume. This study examined the output voltage and power by considering the constant and accelerating plate-rotation speeds, respectively. This paper discusses the effects of the angular acceleration speed of a rotating wheel corresponding to the dynamic behaviors of a weighted rotor. The average output power is 399 to 535 microwatts at plate-rotation speeds from 300 to 500 rpm, enabling the WREH to be a suitable power source for a tire-pressure monitoring system.

  6. A method to design high SNR nanoscale magnetic sensors using an array of tunnelling magneto-resistance (TMR) devices

    Gomez, P; Litvinov, D; Khizroev, S

    2007-01-01

    This paper presents a systematic method to design and calculate tunnelling magneto-resistance (TMR) sensors with high signal-to-noise ratio (SNR). The sensing module consists of four TMR devices arranged in a Wheatstone-bridge configuration. Closed-form equations were obtained to calculate TMR sensor current, array output voltage, magneto-resistance ratio, overall noise (thermal and shot) and SNR for a given bandwidth. Using this technique we were able to maximize the SNR by tuning the many parameters of the TMR devices. Typical SNR values are in excess of 45 dB

  7. Design of a linear detector array unit for high energy x-ray helical computed tomography and linear scanner

    Lee, Jeong Tae; Park, Jong Hwan; Kim, Gi Yoon [Dept. of Nuclear and Quantum Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon (Korea, Republic of); Kim, Dong Geun [Medical Imaging Department, ASTEL Inc., Seongnam (Korea, Republic of); Park, Shin Woong; Yi, Yun [Dept. of Electronics and Information Eng, Korea University, Seoul (Korea, Republic of); Kim, Hyun Duk [Research Center, Luvantix ADM Co., Ltd., Daejeon (Korea, Republic of)

    2016-11-15

    A linear detector array unit (LdAu) was proposed and designed for the high energy X-ray 2-d and 3-d imaging systems for industrial non-destructive test. Specially for 3-d imaging, a helical CT with a 15 MeV linear accelerator and a curved detector is proposed. the arc-shape detector can be formed by many LdAus all of which are arranged to face the focal spot when the source-to-detector distance is fixed depending on the application. An LdAu is composed of 10 modules and each module has 48 channels of CdWO{sub 4} (CWO) blocks and Si PIn photodiodes with 0.4 mm pitch. this modular design was made for easy manufacturing and maintenance. through the Monte carlo simulation, the CWO detector thickness of 17 mm was optimally determined. the silicon PIn photodiodes were designed as 48 channel arrays and fabricated with NTD (neutron transmutation doping) wafers of high resistivity and showed excellent leakage current properties below 1 nA at 10 V reverse bias. to minimize the low-voltage breakdown, the edges of the active layer and the guard ring were designed as a curved shape. the data acquisition system was also designed and fabricated as three independent functional boards; a sensor board, a capture board and a communication board to a Pc. this paper describes the design of the detectors (CWO blocks and Si PIn photodiodes) and the 3-board data acquisition system with their simulation results.

  8. The design of a focused ultrasound transducer array for the treatment of stroke: a simulation study

    Pajek, Daniel; Hynynen, Kullervo

    2012-01-01

    High intensity focused ultrasound (HIFU) is capable of mechanically disintegrating blood clots at high pressures. Safe thrombolysis may require frequencies higher than those currently utilized by transcranial HIFU. Since the attenuation and focal distortion of ultrasound in bone increases at higher frequencies, resulting focal pressures are diminished. This study investigated the feasibility of using transcranial HIFU for the non-invasive treatment of ischemic stroke. The use of large aperture, 1.1–1.5 MHz phased arrays in targeting four clinically relevant vessel locations was simulated. Resulting focal sizes decreased with frequency, producing a maximum –3 dB depth of field and lateral width of 2.0 and 1.2 mm, respectively. Mean focal gains above an order of magnitude were observed in three of four targets and transducer intensities required to achieve thrombolysis were determined. Required transducer element counts are about an order of magnitude higher than what currently exists and so, although technically feasible, new arrays would need to be developed to realize this as a treatment modality for stroke. (paper)

  9. Design and operation of a portable scanner for high performance microchip capillary array electrophoresis.

    Scherer, James R; Liu, Peng; Mathies, Richard A

    2010-11-01

    We have developed a compact, laser-induced fluorescence detection scanner, the multichannel capillary array electrophoresis portable scanner (McCAEPs) as a platform for electrophoretic detection and control of high-throughput, integrated microfluidic devices for genetic and other analyses. The instrument contains a confocal optical system with a rotary objective for detecting four different fluorescence signals, a pneumatic system consisting of two pressure/vacuum pumps and 28 individual addressable solenoid valves for control of on-chip microvalves and micropumps, four Polymerase Chain Reaction (PCR) temperature control systems, and four high voltage power supplies for electrophoresis. The detection limit of the instrument is ~20 pM for on-chip capillary electrophoresis of fluorescein dyes. To demonstrate the system performance for forensic short tandem repeat (STR) analysis, two experiments were conducted: (i) electrophoretic separation and detection of STR samples on a 96-lane microfabricated capillary array electrophoresis microchip. Fully resolved PowerPlex(®) 16 STR profiles amplified from 1 ng of 9947A female standard DNA were successfully obtained; (ii) nine-plex STR amplification, sample injection, separation, and fluorescence detection of 100-copy 9948 male standard DNA in a single integrated PCR- capillary electrophoresis microchip. These results demonstrate that the McCAEPs can be used as a versatile control and detection instrument that operates integrated microfluidic devices for high-performance forensic human identification.

  10. Chromosomes of Iberian Leuciscinae (Cyprinidae) Revisited: Evidence of Genome Restructuring in Homoploid Hybrids Using Dual-Color FISH and CGH

    Pereira, C. S.; Ráb, Petr; Collares-Pereira, M. J.

    2013-01-01

    Roč. 141, 2/3 (2013), s. 143-152 ISSN 1424-8581 R&D Projects: GA ČR GA13-37277S Institutional support: RVO:67985904 Keywords : CGH/GISH * Chondrostoma s.I. * genome reshuffling hybridization Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.905, year: 2013

  11. Comparing temporally-focused GPC and CGH for two-photon excitation and optogenetics in turbid media

    Villangca, Mark Jayson; Bañas, Andrew Rafael; Aabo, Thomas

    2013-01-01

    a 4f setup that directly converts phase information to intensity. The GPC method has been used with temporal focusing for excitation in two-photon optogenetics [1-3]. The computer generated hologram (CGH) is also used to generate arbitrary light patterns and has been used for optical manipulation...

  12. Analysis of Chinese women with primary ovarian insufficiency by high resolution array-comparative genomic hybridization.

    Liao, Can; Fu, Fang; Yang, Xin; Sun, Yi-Min; Li, Dong-Zhi

    2011-06-01

    Primary ovarian insufficiency (POI) is defined as a primary ovarian defect characterized by absent menarche (primary amenorrhea) or premature depletion of ovarian follicles before the age of 40 years. The etiology of primary ovarian insufficiency in human female patients is still unclear. The purpose of this study is to investigate the potential genetic causes in primary amenorrhea patients by high resolution array based comparative genomic hybridization (array-CGH) analysis. Following the standard karyotyping analysis, genomic DNA from whole blood of 15 primary amenorrhea patients and 15 normal control women was hybridized with Affymetrix cytogenetic 2.7M arrays following the standard protocol. Copy number variations identified by array-CGH were confirmed by real time polymerase chain reaction. All the 30 samples were negative by conventional karyotyping analysis. Microdeletions on chromosome 17q21.31-q21.32 with approximately 1.3 Mb were identified in four patients by high resolution array-CGH analysis. This included the female reproductive secretory pathway related factor N-ethylmaleimide-sensitive factor (NSF) gene. The results of the present study suggest that there may be critical regions regulating primary ovarian insufficiency in women with a 17q21.31-q21.32 microdeletion. This effect might be due to the loss of function of the NSF gene/genes within the deleted region or to effects on contiguous genes.

  13. Genome-wide comparison of paired fresh frozen and formalin-fixed paraffin-embedded gliomas by custom BAC and oligonucleotide array comparative genomic hybridization: facilitating analysis of archival gliomas.

    Mohapatra, Gayatry; Engler, David A; Starbuck, Kristen D; Kim, James C; Bernay, Derek C; Scangas, George A; Rousseau, Audrey; Batchelor, Tracy T; Betensky, Rebecca A; Louis, David N

    2011-04-01

    Array comparative genomic hybridization (aCGH) is a powerful tool for detecting DNA copy number alterations (CNA). Because diffuse malignant gliomas are often sampled by small biopsies, formalin-fixed paraffin-embedded (FFPE) blocks are often the only tissue available for genetic analysis; FFPE tissues are also needed to study the intratumoral heterogeneity that characterizes these neoplasms. In this paper, we present a combination of evaluations and technical advances that provide strong support for the ready use of oligonucleotide aCGH on FFPE diffuse gliomas. We first compared aCGH using bacterial artificial chromosome (BAC) arrays in 45 paired frozen and FFPE gliomas, and demonstrate a high concordance rate between FFPE and frozen DNA in an individual clone-level analysis of sensitivity and specificity, assuring that under certain array conditions, frozen and FFPE DNA can perform nearly identically. However, because oligonucleotide arrays offer advantages to BAC arrays in genomic coverage and practical availability, we next developed a method of labeling DNA from FFPE tissue that allows efficient hybridization to oligonucleotide arrays. To demonstrate utility in FFPE tissues, we applied this approach to biphasic anaplastic oligoastrocytomas and demonstrate CNA differences between DNA obtained from the two components. Therefore, BAC and oligonucleotide aCGH can be sensitive and specific tools for detecting CNAs in FFPE DNA, and novel labeling techniques enable the routine use of oligonucleotide arrays for FFPE DNA. In combination, these advances should facilitate genome-wide analysis of rare, small and/or histologically heterogeneous gliomas from FFPE tissues.

  14. A 34K SNP genotyping array for Populus trichocarpa: design, application to the study of natural populations and transferability to other Populus species.

    Geraldes, A; Difazio, S P; Slavov, G T; Ranjan, P; Muchero, W; Hannemann, J; Gunter, L E; Wymore, A M; Grassa, C J; Farzaneh, N; Porth, I; McKown, A D; Skyba, O; Li, E; Fujita, M; Klápště, J; Martin, J; Schackwitz, W; Pennacchio, C; Rokhsar, D; Friedmann, M C; Wasteneys, G O; Guy, R D; El-Kassaby, Y A; Mansfield, S D; Cronk, Q C B; Ehlting, J; Douglas, C J; Tuskan, G A

    2013-03-01

    Genetic mapping of quantitative traits requires genotypic data for large numbers of markers in many individuals. For such studies, the use of large single nucleotide polymorphism (SNP) genotyping arrays still offers the most cost-effective solution. Herein we report on the design and performance of a SNP genotyping array for Populus trichocarpa (black cottonwood). This genotyping array was designed with SNPs pre-ascertained in 34 wild accessions covering most of the species latitudinal range. We adopted a candidate gene approach to the array design that resulted in the selection of 34 131 SNPs, the majority of which are located in, or within 2 kb of, 3543 candidate genes. A subset of the SNPs on the array (539) was selected based on patterns of variation among the SNP discovery accessions. We show that more than 95% of the loci produce high quality genotypes and that the genotyping error rate for these is likely below 2%. We demonstrate that even among small numbers of samples (n = 10) from local populations over 84% of loci are polymorphic. We also tested the applicability of the array to other species in the genus and found that the number of polymorphic loci decreases rapidly with genetic distance, with the largest numbers detected in other species in section Tacamahaca. Finally, we provide evidence for the utility of the array to address evolutionary questions such as intraspecific studies of genetic differentiation, species assignment and the detection of natural hybrids. © 2013 Blackwell Publishing Ltd.

  15. Air shower array designed for cosmic ray variation measurements and high energy gamma ray astronomy

    Morello, C; Navarra, G [Consiglio Nazionale delle Ricerche, Turin (Italy). Lab. di Cosmo-Geofisica

    1981-08-15

    We describe an array for performing measurements of counting rates and arrival directions of extensive air showers at primary energy E/sub 0/ approx. equal to 3 x 10/sup 9/ eV. The aim of the research is to study the time variations and the anisotropies of cosmic rays and the observable gamma ray sources in the high energy region. The installation, composed of four large area scintillation counters and completely controlled by a microcomputer system, operates at mountain altitude (3500 m a.s.l.). The preanalysis of data, stability tests and periodic calibrations are performed by on-line programs. The method for obtaining the required stability and the corrections on temperature and gain variations are also described.

  16. Design of an Acoustic Target Intrusion Detection System Based on Small-Aperture Microphone Array

    Zu, Xingshui; Guo, Feng; Huang, Jingchang; Zhao, Qin; Liu, Huawei; Li, Baoqing; Yuan, Xiaobing

    2017-01-01

    Automated surveillance of remote locations in a wireless sensor network is dominated by the detection algorithm because actual intrusions in such locations are a rare event. Therefore, a detection method with low power consumption is crucial for persistent surveillance to ensure longevity of the sensor networks. A simple and effective two-stage algorithm composed of energy detector (ED) and delay detector (DD) with all its operations in time-domain using small-aperture microphone array (SAMA) is proposed. The algorithm analyzes the quite different velocities between wind noise and sound waves to improve the detection capability of ED in the surveillance area. Experiments in four different fields with three types of vehicles show that the algorithm is robust to wind noise and the probability of detection and false alarm are 96.67% and 2.857%, respectively. PMID:28273838

  17. Getting the Big Picture: Design Considerations for a ngVLA Short Spacing Array

    Mason, Brian Scott; Cotton, William; Condon, James; Kepley, Amanda; Selina, Rob; Murphy, Eric Joseph

    2018-01-01

    The Next Generation VLA (ngVLA) aims to provide a revolutionary increase in cm-wavelength collecting area and sensitivity while at the same time providing excellent image fidelity for a broad spectrum of science cases. Likely ngVLA configurations currently envisioned provide sensitivity over a very wide range of spatial scales. The antenna diameter (notionally 18 meters) fundamentally limits the largest angular scales that can be reached. One simple and powerful way to image larger angular scales is to build a complementary interferometer comprising a smaller number of smaller-diameter dishes.We have investigated the requirements that such an array would need to meet in order to usefully scientifically complement the ngVLA; this poster presents the results of our investigation.

  18. Theory for optimal design of waveguiding light concentrators in photovoltaic microcell arrays.

    Semichaevsky, Andrey V; Johnson, Harley T; Yoon, Jongseung; Nuzzo, Ralph G; Li, Lanfang; Rogers, John

    2011-06-10

    Efficiency of ultrathin flexible solar photovoltaic silicon microcell arrays can be significantly improved using nonimaging solar concentrators. A fluorophore is introduced to match the solar spectrum and the low-reflectivity wavelength range of Si, reduce the escape losses, and allow the nontracking operation. In this paper we optimize our solar concentrators using a luminescent/nonluminescent photon transport model. Key modeling results are compared quantitatively to experiments and are in good agreement with the latter. Our solar concentrator performance is not limited by the dye self-absorption. Bending deformations of the flexible solar collectors do not result in their indirect gain degradation compared to flat solar concentrators with the same projected area.

  19. Design and Implementation of Video Shot Detection on Field Programmable Gate Arrays

    Jharna Majumdar

    2012-09-01

    Full Text Available Video has become an interactive medium of communication in everyday life. The sheer volume of video makes it extremely difficult to browse through and find the required data. Hence extraction of key frames from the video which represents the abstract of the entire video becomes necessary. The aim of the video shot detection is to find the position of the shot boundaries, so that key frames can be selected from each shot for subsequent processing such as video summarization, indexing etc. For most of the surveillance applications like video summery, face recognition etc., the hardware (real time implementation of these algorithms becomes necessary. Here in this paper we present the architecture for simultaneous accessing of consecutive frames, which are then used for the implementation of various Video Shot Detection algorithms. We also present the real time implementation of three video shot detection algorithms using the above mentioned architecture on FPGA (Field Programmable Gate Arrays.

  20. Improved Titanium Billet Inspection Sensitivity through Optimized Phased Array Design, Part II: Experimental Validation and Comparative Study with Multizone

    Hassan, W.; Vensel, F.; Knowles, B.; Lupien, V.

    2006-01-01

    The inspection of critical rotating components of aircraft engines has made important advances over the last decade. The development of Phased Array (PA) inspection capability for billet and forging materials used in the manufacturing of critical engine rotating components has been a priority for Honeywell Aerospace. The demonstration of improved PA inspection system sensitivity over what is currently used at the inspection houses is a critical step in the development of this technology and its introduction to the supply base as a production inspection. As described in Part I (in these proceedings), a new phased array transducer was designed and manufactured for optimal inspection of eight inch diameter Ti-6Al-4V billets. After confirming that the transducer was manufactured in accordance with the design specifications a validation study was conducted to assess the sensitivity improvement of the PAI over the current capability of Multi-zone (MZ) inspection. The results of this study confirm the significant (≅ 6 dB in FBH number sign sensitivity) improvement of the PAI sensitivity over that of MZI

  1. Design and Performance of the Multiplexed SQUID/TES Array at Ninety Gigahertz

    Stanchfield, Sara; Ade, Peter; Aguirre, James; Brevik, Justus A.; Cho, Hsiao-Mei; Datta, Rahul; Devlin, Mark; Dicker, Simon R.; Dober, Bradley; Duff, Shannon M.; Egan, Dennis; Ford, Pam; Hilton, Gene; Hubmayr, Johannes; Irwin, Kent; Knowles, Kenda; Marganian, Paul; Mason, Brian Scott; Mates, John A. B.; McMahon, Jeff; Mello, Melinda; Mroczkowski, Tony; Romero, Charles; Sievers, Jonathon; Tucker, Carole; Vale, Leila R.; Vissers, Michael; White, Steven; Whitehead, Mark; Ullom, Joel; Young, Alexander

    2018-01-01

    We present the array performance and astronomical images from early science results from MUSTANG-2, a 90 GHz feedhorn-coupled, microwave SQUID-multiplexed TES bolometer array operating on the Robert C. Byrd Green Bank Telescope (GBT). MUSTANG-2 was installed on the GBT on December 2, 2016 and immediately began commissioning efforts, followed by science observations, which are expected to conclude June 2017. The feedhorn and waveguide-probe-coupled detector technology is a mature technology, which has been used on instrument including the South Pole Telescope, the Atacama Cosmology Telescope, and the Atacama B-mode Search telescope. The microwave SQUID readout system developed for MUSTANG-2 currently reads out 66 detectors with a single coaxial cable and will eventually allow thousands of detectors to be multiplexed. This microwave SQUID multiplexer combines the proven abilities of millimeterwave TES detectors with the multiplexing capabilities of KIDs with no degradation in noise performance of the detectors. Each multiplexing device is read out using warm electronics consisting of a commercially available ROACH board, a DAC/ADC card, and an Intermediate Frequency mixer circuit. The hardware was originally developed by the UC Berkeley Collaboration for Astronomy Signal Processing and Electronic Research (CASPER) group, whose primary goal is to develop scalable FPGA-based hardware with the flexibility to be used in a wide range of radio signal processing applications. MUSTANG-2 is the first on-sky instrument to use microwave SQUID multiplexing and is available as a shared-risk/PI instrument on the GBT. In MUSTANG-2's first season 7 separate proposals were awarded a total of 230 hours of telescope time.

  2. Analysis of Reverse Phase Protein Array Data: From Experimental Design towards Targeted Biomarker Discovery

    Astrid Wachter

    2015-11-01

    Full Text Available Mastering the systematic analysis of tumor tissues on a large scale has long been a technical challenge for proteomics. In 2001, reverse phase protein arrays (RPPA were added to the repertoire of existing immunoassays, which, for the first time, allowed a profiling of minute amounts of tumor lysates even after microdissection. A characteristic feature of RPPA is its outstanding sample capacity permitting the analysis of thousands of samples in parallel as a routine task. Until today, the RPPA approach has matured to a robust and highly sensitive high-throughput platform, which is ideally suited for biomarker discovery. Concomitant with technical advancements, new bioinformatic tools were developed for data normalization and data analysis as outlined in detail in this review. Furthermore, biomarker signatures obtained by different RPPA screens were compared with another or with that obtained by other proteomic formats, if possible. Options for overcoming the downside of RPPA, which is the need to steadily validate new antibody batches, will be discussed. Finally, a debate on using RPPA to advance personalized medicine will conclude this article.

  3. Cross-species comparison of aCGH data from mouse and human BRCA1- and BRCA2-mutated breast cancers

    Holstege, Henne; Wessels, Lodewyk FA; Nederlof, Petra M; Jonkers, Jos; Beers, Erik van; Velds, Arno; Liu, Xiaoling; Joosse, Simon A; Klarenbeek, Sjoerd; Schut, Eva; Kerkhoven, Ron; Klijn, Christiaan N

    2010-01-01

    Genomic gains and losses are a result of genomic instability in many types of cancers. BRCA1- and BRCA2-mutated breast cancers are associated with increased amounts of chromosomal aberrations, presumably due their functions in genome repair. Some of these genomic aberrations may harbor genes whose absence or overexpression may give rise to cellular growth advantage. So far, it has not been easy to identify the driver genes underlying gains and losses. A powerful approach to identify these driver genes could be a cross-species comparison of array comparative genomic hybridization (aCGH) data from cognate mouse and human tumors. Orthologous regions of mouse and human tumors that are commonly gained or lost might represent essential genomic regions selected for gain or loss during tumor development. To identify genomic regions that are associated with BRCA1- and BRCA2-mutated breast cancers we compared aCGH data from 130 mouse Brca1 Δ/Δ ;p53 Δ/Δ , Brca2 Δ/Δ ;p53 Δ/Δ and p53 Δ/Δ mammary tumor groups with 103 human BRCA1-mutated, BRCA2-mutated and non-hereditary breast cancers. Our genome-wide cross-species analysis yielded a complete collection of loci and genes that are commonly gained or lost in mouse and human breast cancer. Principal common CNAs were the well known MYC-associated gain and RB1/INTS6-associated loss that occurred in all mouse and human tumor groups, and the AURKA-associated gain occurred in BRCA2-related tumors from both species. However, there were also important differences between tumor profiles of both species, such as the prominent gain on chromosome 10 in mouse Brca2 Δ/Δ ;p53 Δ/Δ tumors and the PIK3CA associated 3q gain in human BRCA1-mutated tumors, which occurred in tumors from one species but not in tumors from the other species. This disparity in recurrent aberrations in mouse and human tumors might be due to differences in tumor cell type or genomic organization between both species. The selection of the oncogenome during

  4. The CHARA array adaptive optics I: common-path optical and mechanical design, and preliminary on-sky results

    Che, Xiao; Sturmann, Laszlo; Monnier, John D.; ten Brummelaar, Theo A.; Sturmann, Judit; Ridgway, Stephen T.; Ireland, Michael J.; Turner, Nils H.; McAlister, Harold A.

    2014-07-01

    The CHARA array is an optical interferometer with six 1-meter diameter telescopes, providing baselines from 33 to 331 meters. With sub-milliarcsecond angular resolution, its versatile visible and near infrared combiners offer a unique angle of studying nearby stellar systems by spatially resolving their detailed structures. To improve the sensitivity and scientific throughput, the CHARA array was funded by NSF-ATI in 2011 to install adaptive optics (AO) systems on all six telescopes. The initial grant covers Phase I of the AO systems, which includes on-telescope Wavefront Sensors (WFS) and non-common-path (NCP) error correction. Meanwhile we are seeking funding for Phase II which will add large Deformable Mirrors on telescopes to close the full AO loop. The corrections of NCP error and static aberrations in the optical system beyond the WFS are described in the second paper of this series. This paper describes the design of the common-path optical system and the on-telescope WFS, and shows the on-sky commissioning results.

  5. Analyses of Genotypes and Phenotypes of Ten Chinese Patients with Wolf-Hirschhorn Syndrome by Multiplex Ligation-dependent Probe Amplification and Array Comparative Genomic Hybridization

    Wen-Xu Yang

    2016-01-01

    Conclusions: The combined use of MLPA and array CGH is an effective and specific means to diagnose WHS and allows for the precise identification of the breakpoints and sizes of deletions. The deletion of genes in the WHS candidate region is closely correlated with the core WHS phenotype.

  6. Design, Construction, and Initial Test of High Spatial Resolution Thermometry Arrays for Detection of Surface Temperature Profiles on SRF Cavities in Super Fluid Helium

    Ari Palczewski, Rongli Geng, Grigory Eremeev

    2011-07-01

    We designed and built two high resolution (0.6-0.55mm special resolution [1.1-1.2mm separation]) thermometry arrays prototypes out of the Allen Bradley 90-120 ohm 1/8 watt resistor to measure surface temperature profiles on SRF cavities. One array was designed to be physically flexible and conform to any location on a SRF cavity; the other was modeled after the common G-10/stycast 2850 thermometer and designed to fit on the equator of an ILC (Tesla 1.3GHz) SRF cavity. We will discuss the advantages and disadvantages of each array and their construction. In addition we will present a case study of the arrays performance on a real SRF cavity TB9NR001. TB9NR001 presented a unique opportunity to test the performance of each array as it contained a dual (4mm separation) cat eye defect which conventional methods such as OST (Oscillating Superleak second-sound Transducers) and full coverage thermometry mapping were unable to distinguish between. We will discuss the new arrays ability to distinguish between the two defects and their preheating performance.

  7. Polar body array CGH for prediction of the status of the corresponding oocyte. Part II: technical aspects

    Magli, M. Cristina; Montag, Markus; Köster, Maria; Muzi, Luigi; Geraedts, Joep; Collins, John; Goossens, Veerle; Handyside, Alan H.; Harper, Joyce; Repping, Sjoerd; Schmutzler, Andreas; Vesela, Katerina; Gianaroli, Luca

    2011-01-01

    The purpose of this study was to assess the technical aspects related to polar body (PB) biopsy, which might have an influence on the results of the microarray comparative genomic hybridization analysis. Furthermore, a comparison was made between two biopsy methods (mechanical and laser). Biopsy of

  8. Polar body array CGH for prediction of the status of the corresponding oocyte. Part I: clinical results

    Geraedts, Joep; Montag, Markus; Magli, M. Cristina; Repping, Sjoerd; Handyside, Alan; Staessen, Catherine; Harper, Joyce; Schmutzler, Andreas; Collins, John; Goossens, Veerle; van der Ven, Hans; Vesela, Katerina; Gianaroli, Luca

    2011-01-01

    Several randomized controlled trials have not shown a benefit from preimplantation genetic screening (PGS) biopsy of cleavage-stage embryos and assessment of up to 10 chromosomes for aneuploidy. Therefore, a proof-of-principle study was planned to determine the reliability of alternative form of

  9. Optimal design method for magnetization directions of a permanent magnet array

    Choi, Jae Seok [Center for Information Storage Device, Yonsei University, 262 Seongsanno, Seodaemun-gu, Seoul 120-749 (Korea, Republic of); Yoo, Jeonghoon, E-mail: yoojh@yonsei.ac.k [School of Mechanical Engineering, Yonsei University, 262 Seongsanno, Seodaemun-gu, Seoul 120-749 (Korea, Republic of)

    2010-08-15

    In many magnetic systems, the permanent magnet (PM) pattern has a great influence on their performance. This study proposes a systematic optimization method for designing discrete magnetization directions. While previous works have been mostly dependent on researchers' intuition, the developed method is systematic and can be applied to a two-dimensional PM-type eddy current brake model. The effectiveness of the method is confirmed, where the design's aim is to maximize the braking force on a moving conductor. The sensitivity analysis is accomplished by the adjoint variable method and the sequential linear programming is used as an optimizer. Several optimization results for various conditions through the proposed design method are compared to each other and the optimal magnet configuration for an eddy current brake is suggested.

  10. Programmable logic controller performance enhancement by field programmable gate array based design.

    Patel, Dhruv; Bhatt, Jignesh; Trivedi, Sanjay

    2015-01-01

    PLC, the core element of modern automation systems, due to serial execution, exhibits limitations like slow speed and poor scan time. Improved PLC design using FPGA has been proposed based on parallel execution mechanism for enhancement of performance and flexibility. Modelsim as simulation platform and VHDL used to translate, integrate and implement the logic circuit in FPGA. Xilinx's Spartan kit for implementation-testing and VB has been used for GUI development. Salient merits of the design include cost-effectiveness, miniaturization, user-friendliness, simplicity, along with lower power consumption, smaller scan time and higher speed. Various functionalities and applications like typical PLC and industrial alarm annunciator have been developed and successfully tested. Results of simulation, design and implementation have been reported. Copyright © 2014 ISA. Published by Elsevier Ltd. All rights reserved.

  11. Intragenic rearrangements in X-linked intellectual deficiency: results of a-CGH in a series of 54 patients and identification of TRPC5 and KLHL15 as potential XLID genes.

    Mignon-Ravix, Cécile; Cacciagli, Pierre; Choucair, Nancy; Popovici, Cornel; Missirian, Chantal; Milh, Mathieu; Mégarbané, André; Busa, Tiffany; Julia, Sophie; Girard, Nadine; Badens, Catherine; Sigaudy, Sabine; Philip, Nicole; Villard, Laurent

    2014-08-01

    High-resolution array comparative genomic hybridization (a-CGH) enables the detection of intragenic rearrangements, such as single exon deletion or duplication. This approach can lead to the identification of new disease genes. We report on the analysis of 54 male patients presenting with intellectual deficiency (ID) and a family history suggesting X-linked (XL) inheritance or maternal skewed X-chromosome inactivation (XCI), using a home-made X-chromosome-specific microarray covering the whole human X-chromosome at high resolution. The majority of patients had whole genome array-CGH prior to the selection and we did not include large rearrangements such as MECP2 and FMR1 duplications. We identified four rearrangements considered as causative or potentially pathogenic, corresponding to a detection rate of 8%. Two CNVs affected known XLID genes and were therefore considered as causative (IL1RAPL1 and OPHN1 intragenic deletions). Two new CNVs were considered as potentially pathogenic as they affected interesting candidates for ID. The first CNV is a deletion of the first exon of the TRPC5 gene, encoding a cation channel implicated in dendrite growth and patterning, in a child presenting with ID and an autism spectrum disorder (ASD). The second CNV is a partial deletion of KLHL15, in a patient with severe ID, epilepsy, and anomalies of cortical development. In both cases, in spite of strong arguments for clinical relevance, we were not able at this stage to confirm pathogenicity of the mutations, and the causality of the variants identified in XLID remains to be confirmed. © 2014 Wiley Periodicals, Inc.

  12. Objective assessment and design improvement of a staring, sparse transducer array by the spatial crosstalk matrix for 3D photoacoustic tomography.

    Philip Wong

    Full Text Available Accurate reconstruction of 3D photoacoustic (PA images requires detection of photoacoustic signals from many angles. Several groups have adopted staring ultrasound arrays, but assessment of array performance has been limited. We previously reported on a method to calibrate a 3D PA tomography (PAT staring array system and analyze system performance using singular value decomposition (SVD. The developed SVD metric, however, was impractical for large system matrices, which are typical of 3D PAT problems. The present study consisted of two main objectives. The first objective aimed to introduce the crosstalk matrix concept to the field of PAT for system design. Figures-of-merit utilized in this study were root mean square error, peak signal-to-noise ratio, mean absolute error, and a three dimensional structural similarity index, which were derived between the normalized spatial crosstalk matrix and the identity matrix. The applicability of this approach for 3D PAT was validated by observing the response of the figures-of-merit in relation to well-understood PAT sampling characteristics (i.e. spatial and temporal sampling rate. The second objective aimed to utilize the figures-of-merit to characterize and improve the performance of a near-spherical staring array design. Transducer arrangement, array radius, and array angular coverage were the design parameters examined. We observed that the performance of a 129-element staring transducer array for 3D PAT could be improved by selection of optimal values of the design parameters. The results suggested that this formulation could be used to objectively characterize 3D PAT system performance and would enable the development of efficient strategies for system design optimization.

  13. Development of an Accelerated Test Design for Predicting the Service Life of the Solar Array at Mead, Nebraska

    Gaines, G. B.; Thomas, R. E.; Noel, G. T.; Shilliday, T. S.; Wood, V. E.; Carmichael, D. C.

    1979-01-01

    An accelerated life test is described which was developed to predict the life of the 25 kW photovoltaic array installed near Mead, Nebraska. A quantitative model for accelerating testing using multiple environmental stresses was used to develop the test design. The model accounts for the effects of thermal stress by a relation of the Arrhenius form. This relation was then corrected for the effects of nonthermal environmental stresses, such as relative humidity, atmospheric pollutants, and ultraviolet radiation. The correction factors for the nonthermal stresses included temperature-dependent exponents to account for the effects of interactions between thermal and nonthermal stresses on the rate of degradation of power output. The test conditions, measurements, and data analyses for the accelerated tests are presented. Constant-temperature, cyclic-temperature, and UV types of tests are specified, incorporating selected levels of relative humidity and chemical contamination and an imposed forward-bias current and static electric field.

  14. Facile fabrication and configuration design of Co3O4 porous acicular nanorod arrays on Ni foam for supercapacitors.

    Jiang, Tongtong; Yang, Siyu; Bai, Zhiman; Dai, Peng; Yu, Xinxin; Wu, Mingzai; Hu, Haibo

    2018-05-11

    The configuration of electrode materials is of great significance to the performance of supercapacitors (SCs) because of its direct effects on specific surface area and electron transfer path. Given this, herein, a series of Co3O4 hierarchical configurations composed of porous acicular nanorods are designedly synthesized on Ni foam with in-site self-organization method depending on the addition of NH4F. In the absence of NH4F, Co3O4 nanorods self-assemble into porous urchin-like structure (PULS), while the introduction of NH4F can induce the vertical growth of Co3O4 acicular nanorods, forming porous acicular nanorod arrays (PANRAs). By simply tuning the concentration of NH4F, the Co3O4 PANRAs with different specific surface area can be obtained. As expected, Co3O4 PANRAs electrode for SCs (using 1 mmol of NH4F) exhibits high specific capacitance (1486 F g-1 at 1 A g-1) and excellent cycling stability (98.8 % retention after 5000 continuous charge-discharge cycles), which are better than those of Co3O4 PULS electrode (658.2 F g-1 at 1 A g-1, 90.4 %). Corresponding solid-state symmetric SC achieves a high energy density of 48.63 Wh kg-1 at power density of 600 W kg-1. Such superior performance is attributed to fast charge-transfer kinetics, facile electron transport and ions diffusion rate resulting from porous array structure, indicating the importance of configuration design of electrode materials for high performance SCs. © 2018 IOP Publishing Ltd.

  15. The Bepicolombo Mercury Planetary Orbiter (MPO Solar Array Design, Major Developments and Qualification

    Loehberg A.

    2017-01-01

    The MPO solar generator is composed of one wing consisting of three panels and provides an average power output up to 1800W during the nominal 1 Earth year mission around Mercury. The wing design is characterised by temperature reduction measures. The flight wing has already passed the majority of the environmental test program.

  16. Design of RS232-powered controller for switched parasitic array antenna

    Mofolo, MOR

    2013-09-01

    Full Text Available It is often convenient to power up peripheral devices directly from the host device interface, without a need for additional and/or external power supply. In this paper we present a design of the RS232-powered controller for electronically...

  17. Design and experimental validation of Unilateral Linear Halbach magnet arrays for single-sided magnetic resonance.

    Bashyam, Ashvin; Li, Matthew; Cima, Michael J

    2018-07-01

    Single-sided NMR has the potential for broad utility and has found applications in healthcare, materials analysis, food quality assurance, and the oil and gas industry. These sensors require a remote, strong, uniform magnetic field to perform high sensitivity measurements. We demonstrate a new permanent magnet geometry, the Unilateral Linear Halbach, that combines design principles from "sweet-spot" and linear Halbach magnets to achieve this goal through more efficient use of magnetic flux. We perform sensitivity analysis using numerical simulations to produce a framework for Unilateral Linear Halbach design and assess tradeoffs between design parameters. Additionally, the use of hundreds of small, discrete magnets within the assembly allows for a tunable design, improved robustness to variability in magnetization strength, and increased safety during construction. Experimental validation using a prototype magnet shows close agreement with the simulated magnetic field. The Unilateral Linear Halbach magnet increases the sensitivity, portability, and versatility of single-sided NMR. Copyright © 2018 Elsevier Inc. All rights reserved.

  18. A comparative design view for accurate control of servos using a field programmable gate array

    Tickle, A J; Harvey, P K; Smith, J S; Wu, F; Buckle, J R

    2009-01-01

    An embedded system is a special-purpose computer system designed to perform one or a few dedicated functions. Altera DSP Builder presents designers and users with an alternate approach when creating their systems by employing a blockset similar to that already used in Simulink. The application considered in this paper is the design of a Pulse Width Modulation (PWM) system for use in stereo vision. PWM can replace a digital-to-analogue converter to control audio speakers, LED intensity, motor speed, and servo position. Rather than the conventional HDL coding approach this Simulink approach provides an easy understanding platform to the PWM design. This paper includes a comparison between two approaches regarding resource usage and flexibility etc. Included is how DSP Builder manipulates an onboard clock signal, in order to create the control pulses to the 'raw' coding of a PWM generator in VHDL. Both methods were shown to a selection of people and their views on which version they would subsequently use in their relative fields is discussed.

  19. Array Comparative Genomic Hybridization as the First-line Investigation for Neonates with Congenital Heart Disease: Experience in a Single Tertiary Center.

    Choi, Bo Geum; Hwang, Su Kyung; Kwon, Jung Eun; Kim, Yeo Hyang

    2018-03-01

    The purpose of the present study was to investigate the advantages and disadvantages of verifying genetic abnormalities using array comparative genomic hybridization (a-CGH) immediately after diagnosis of congenital heart disease (CHD). Among neonates under the age of 28 days who underwent echocardiography from January 1, 2014 to April 30, 2016, neonates whose chromosomal and genomic abnormalities were tested using a-CGH in cases of an abnormal finding on echocardiography were enrolled. Of the 166 patients diagnosed with CHD, 81 underwent a-CGH and 11 patients (11/81, 13.5%) had abnormal findings on a-CGH. 22q11.2 deletion syndrome was the most common (4/11, 36.4%). On the first a-CGH, 4 patients were negative (4/81, 5%). Three of them were finally diagnosed with Williams syndrome using fluorescent in situ hybridization (FISH), 1 patient was diagnosed with Noonan syndrome through exome sequencing. All of them exhibited diffuse pulmonary artery branch hypoplasia, as well as increased velocity of blood flow, on repeated echocardiography. Five patients started rehabilitation therapy at mean 6 months old age in outpatient clinics and epilepsy was diagnosed in 2 patients. Parents of 2 patients (22q11.2 deletion syndrome and Patau syndrome) refused treatment due to the anticipated prognosis. Screening tests for genetic abnormalities using a-CGH in neonates with CHD has the advantage of early diagnosis of genetic abnormality during the neonatal period in which there is no obvious symptom of genetic abnormality. However, there are disadvantages that some genetic abnormalities cannot be identified on a-CGH. Copyright © 2018. The Korean Society of Cardiology.

  20. Low concentration ratio solar array for low Earth orbit multi-100 kW application. Volume 1: Design, analysis and development tests

    1983-01-01

    A preliminary design effort directed toward a low concentration ratio photovoltaic array system capable of delivering multihundred kilowatts (300 kW to 1000 kW range) in low earth orbit is described. The array system consists of two or more array modules each capable of delivering between 113 kW to 175 kW using silicon solar cells or gallium arsenide solar cells, respectively. The array module deployed area is 1320 square meters and consists of 4356 pyramidal concentrator elements. The module, when stowed in the Space Shuttle's payload bay, has a stowage volume of a cube with 3.24 meters on a side. The concentrator elements are sized for a geometric concentration ratio (GCR) of six with an aperture area of .25 sq. m. The structural analysis and design trades leading to the baseline design are discussed. It describes the configuration, as well as optical, thermal and electrical performance analyses that support the design and overall performance estimates for the array are described.

  1. SPAD array based TOF SoC design for unmanned vehicle

    Pan, An; Xu, Yuan; Xie, Gang; Huang, Zhiyu; Zheng, Yanghao; Shi, Weiwei

    2018-03-01

    As for the requirement of unmanned-vehicle mobile Lidar system, this paper presents a SoC design based on pulsed TOF depth image sensor. This SoC has a detection range of 300m and detecting resolution of 1.5cm. Pixels are made of SPAD. Meanwhile, SoC adopts a structure of multi-pixel sharing TDC, which significantly reduces chip area and improve the fill factor of light-sensing surface area. SoC integrates a TCSPC module to achieve the functionality of receiving each photon, measuring photon flight time and processing depth information in one chip. The SOC is designed in the SMIC 0.13μm CIS CMOS technology

  2. A Parameterized Pattern-Error Objective for Large-Scale Phase-Only Array Pattern Design

    2016-03-21

    of 1D heuristic design approaches can be found in [6–11]. Reference [12], which proposes separate beams on interlaced subarrays, represents a 2D...and is well suited for solving with efficient quasi-Newton methods. Although deliberately simple, the objective has several heuristic parameters for...with efficient quasi-Newton methods. Although deliberately simple, the objective has several heuristic parameters for flexibility and can be used to

  3. The flush-mounted rail Langmuir probe array designed for the Alcator C-Mod vertical target plate divertor

    Kuang, A. Q.; Brunner, D.; LaBombard, B.; Leccacorvi, R.; Vieira, R.

    2018-04-01

    An array of flush-mounted and toroidally elongated Langmuir probes (henceforth called rail probes) have been specifically designed for the Alcator C-Mod's vertical target plate divertor and operated over multiple campaigns. The "flush" geometry enables the tungsten electrodes to survive high heat flux conditions in which traditional "proud" tungsten electrodes suffer damage from melting. The toroidally elongated rail-like geometry reduces the influence of sheath expansion, which is an important effect to consider in the design and interpretation of flush-mounted Langmuir probes. The new rail probes successfully operated during C-Mod's FY2015 and FY2016 experimental campaigns with no evidence of damage, despite being regularly subjected to heat flux densities parallel to the magnetic field exceeding ˜1 GW m-2 for short periods of time. A comparison between rail and proud probe data indicates that sheath expansion effects were successfully mitigated by the rail design, extending the use of these Langmuir probes to incident magnetic field line angles as low as 0.5°.

  4. Image processing system design for microcantilever-based optical readout infrared arrays

    Tong, Qiang; Dong, Liquan; Zhao, Yuejin; Gong, Cheng; Liu, Xiaohua; Yu, Xiaomei; Yang, Lei; Liu, Weiyu

    2012-12-01

    Compared with the traditional infrared imaging technology, the new type of optical-readout uncooled infrared imaging technology based on MEMS has many advantages, such as low cost, small size, producing simple. In addition, the theory proves that the technology's high thermal detection sensitivity. So it has a very broad application prospects in the field of high performance infrared detection. The paper mainly focuses on an image capturing and processing system in the new type of optical-readout uncooled infrared imaging technology based on MEMS. The image capturing and processing system consists of software and hardware. We build our image processing core hardware platform based on TI's high performance DSP chip which is the TMS320DM642, and then design our image capturing board based on the MT9P031. MT9P031 is Micron's company high frame rate, low power consumption CMOS chip. Last we use Intel's company network transceiver devices-LXT971A to design the network output board. The software system is built on the real-time operating system DSP/BIOS. We design our video capture driver program based on TI's class-mini driver and network output program based on the NDK kit for image capturing and processing and transmitting. The experiment shows that the system has the advantages of high capturing resolution and fast processing speed. The speed of the network transmission is up to 100Mbps.

  5. Explorative data analysis of MCL reveals gene expression networks implicated in survival and prognosis supported by explorative CGH analysis

    Blenk, Steffen; Engelmann, Julia C; Pinkert, Stefan; Weniger, Markus; Schultz, Jörg; Rosenwald, Andreas; Müller-Hermelink, Hans K; Müller, Tobias; Dandekar, Thomas

    2008-01-01

    Mantle cell lymphoma (MCL) is an incurable B cell lymphoma and accounts for 6% of all non-Hodgkin's lymphomas. On the genetic level, MCL is characterized by the hallmark translocation t(11;14) that is present in most cases with few exceptions. Both gene expression and comparative genomic hybridization (CGH) data vary considerably between patients with implications for their prognosis. We compare patients over and below the median of survival. Exploratory principal component analysis of gene expression data showed that the second principal component correlates well with patient survival. Explorative analysis of CGH data shows the same correlation. On chromosome 7 and 9 specific genes and bands are delineated which improve prognosis prediction independent of the previously described proliferation signature. We identify a compact survival predictor of seven genes for MCL patients. After extensive re-annotation using GEPAT, we established protein networks correlating with prognosis. Well known genes (CDC2, CCND1) and further proliferation markers (WEE1, CDC25, aurora kinases, BUB1, PCNA, E2F1) form a tight interaction network, but also non-proliferative genes (SOCS1, TUBA1B CEBPB) are shown to be associated with prognosis. Furthermore we show that aggressive MCL implicates a gene network shift to higher expressed genes in late cell cycle states and refine the set of non-proliferative genes implicated with bad prognosis in MCL. The results from explorative data analysis of gene expression and CGH data are complementary to each other. Including further tests such as Wilcoxon rank test we point both to proliferative and non-proliferative gene networks implicated in inferior prognosis of MCL and identify suitable markers both in gene expression and CGH data

  6. Theory, Design, and Measurement of Novel Uniform Circular Antenna Arrays for Direction of Arrival Estimation

    2015-01-01

    Queen in Right of Canada, as represented by the Minister of National Defence, 2015 c⃝ Sa Majesté la Reine (en droit du Canada), telle que réprésentée... publication in [1]. Sections 5 and 6 describe the design and fabrication of the three novel UCAs for DOA estimation and provide simulation results. Section...DoA resolution limits in MIMO channel sounding,” in 2004 IEEE International Symposium On Antennas and Propagation and USNC/URSI National Radio

  7. Clinical trial involving sufferers and non-sufferers of cervicogenic headache (CGH): potential mechanisms of action of photobiomodulation (Conference Presentation)

    Liebert, Ann D.; Bicknell, Brian

    2017-02-01

    Photobiomodulation (PBM) is an effective tool for the management of spinal pain including inflammation of facet joints. Apart from cervical and lumbar joint pain the upper cervical spine facet joint inflammation can result in the CGH (traumatic or atraumatic in origin). This condition affects children, adults and elders and is responsible for 19% of chronic headache and up to 33% of patients in pain clinics. The condition responds well to physiotherapy, facet joint injection, radiofrequency neurotomy and surgery at a rate of 75%. The other 25% being unresponsive to treatment with no identified features of unresponsiveness. In other conditions of chronic unresponsive cervical pain have responded to photobiomodulation at a level of 80% in the short and medium term. A clinical trial was therefore conducted on a cohort of atraumatic patients from the ages of 5-93 (predominantly Neurologist referred / familial sufferers 2/3 generations vertically and laterally) who had responded to a course of PBM and physiotherapy. The CGH sufferers and their non CGH suffering relatives over these generations were then compared for features that distinguish the two groups. Fifty parameters were tested (anthropmetric, movement and neural tension tests included) and there was a noted difference in tandem stance between the groups (.04 significance with repeated measures). As this impairment is common to benign ataxia and migrainous vertigo and in these conditions there is an ion channelopathy (especially potassium channelopathy). A postulated mechanism of action of PBM would involve modulation of ion channels and this is discussed in this presentation.

  8. INFLUENCE OF IONIZING IRRADIATION ON THE PARAMETERS OF ZN NANOTUBES ARRAYS FOR DESIGN OF FLEXIBLE ELECTRONICS ELEMENTS

    D. B. Kadyrzhanov

    2018-01-01

    Full Text Available The aim of the study is establishing the possibility of using Zn nanotube arrays as a basis for design compact and lightweight elements of flexible electronics, including operating under influence of ionizing irradiation.The paper presents the results of the synthesis of Zn nanotubes obtained by electrochemical deposition in the pores of polymer matrices and the study of their structural and electrophysical properties after directional modification by ionizing radiation with different doses. Using the methods of scanning electron microscopy, X-ray diffraction and energy dispersive analysis, the structure of nanotubes having a polycrystalline structure and completely consisting of zinc was studied and it was demonstrated that irradiation with Ar8+ ions with a dose from 1 × 109 to 5 × 1011 ion/cm2 and energy 1.75 MeV/nucleon has an effect on the crystal structure of nanotubes.At high doses, localized highly defect zones arise, leading to a critical change in the structure and physical properties of the nanotubes, respectively. It is shown that the consequence of the modification of the crystal structure is a change in the electrical conductivity of nanotubes: at low doses the electrical conductivity increases, but when the threshold value is reached, it sharply decreases. The change in the crystal structure and the corresponding changes in the conductive properties of Zn nanotubes due to irradiation determine the mechanism of ionizing radiation influence on nanomaterials and determine the possibility of using Zn nanotubes arrays as a basis for creating compact and lightweight elements of flexible electronics.

  9. Design, fabrication and characterization of an arrayable all-polymer microfluidic valve employing highly magnetic rare-earth composite polymer

    Rahbar, Mona; Gray, Bonnie L; Shannon, Lesley

    2016-01-01

    We present a new magnetically actuated microfluidic valve that employs a highly magnetic composite polymer (M-CP) containing rare-earth hard-magnetic powder for its actuating element and for its valve seat. The M-CP offers much higher magnetization compared to the soft-magnetic, ferrite-based composite polymers typically used in microfluidic applications. Each valve consists of a permanently magnetized M-CP flap and valve seat mounted on a microfluidic channel system fabricated in poly(dimethylsiloxane) (PDMS). Each valve is actuated under a relatively small external magnetic field of 80 mT provided by a small permanent magnet mounted on a miniature linear actuator. The performance of the valve with different flap thicknesses is characterized. In addition, the effect of the magnetic valve seat on the valve’s performance is also characterized. It is experimentally shown that a valve with a 2.3 mm flap thickness, actuated under an 80 mT magnetic field, is capable of completely blocking liquid flow at a flow rate of 1 ml min −1 for pressures up to 9.65 kPa in microfluidic channels 200 μ m wide and 200 μ m deep. The valve can also be fabricated into an array for flow switching between multiple microfluidic channels under continuous flow conditions. The performance of arrays of valves for flow routing is demonstrated for flow rates up to 5 ml min −1 with larger microfluidic channels of up to 1 mm wide and 500 μ m deep. The design of the valves is compatible with other commonly used polymeric microfluidic components, as well as other components that use the same novel permanently magnetic composite polymer, such as our previously reported cilia-based mixing devices. (paper)

  10. Transition-Edge Sensor Pixel Parameter Design of the Microcalorimeter Array for the X-Ray Integral Field Unit on Athena

    Smith, S. J.; Adams, J. S.; Bandler, S. R.; Betancourt-Martinez, G. L.; Chervenak, J. A.; Chiao, M. P.; Eckart, M. E.; Finkbeiner, F. M.; Kelley, R. L.; Kilbourne, C. A.; hide

    2016-01-01

    The focal plane of the X-ray integral field unit (X-IFU) for ESA's Athena X-ray observatory will consist of approximately 4000 transition edge sensor (TES) x-ray microcalorimeters optimized for the energy range of 0.2 to 12 kiloelectronvolts. The instrument will provide unprecedented spectral resolution of approximately 2.5 electronvolts at energies of up to 7 kiloelectronvolts and will accommodate photon fluxes of 1 milliCrab (90 counts per second) for point source observations. The baseline configuration is a uniform large pixel array (LPA) of 4.28 arcseconds pixels that is read out using frequency domain multiplexing (FDM). However, an alternative configuration under study incorporates an 18 by × 18 small pixel array (SPA) of 2 arcseconds pixels in the central approximately 36 arcseconds region. This hybrid array configuration could be designed to accommodate higher fluxes of up to 10 milliCrabs (900 counts per second) or alternately for improved spectral performance (less than 1.5 electronvolts) at low count-rates. In this paper we report on the TES pixel designs that are being optimized to meet these proposed LPA and SPA configurations. In particular we describe details of how important TES parameters are chosen to meet the specific mission criteria such as energy resolution, count-rate and quantum efficiency, and highlight performance trade-offs between designs. The basis of the pixel parameter selection is discussed in the context of existing TES arrays that are being developed for solar and x-ray astronomy applications. We describe the latest results on DC biased diagnostic arrays as well as large format kilo-pixel arrays and discuss the technical challenges associated with integrating different array types on to a single detector die.

  11. Transition-edge sensor pixel parameter design of the microcalorimeter array for the x-ray integral field unit on Athena

    Smith, S. J.; Adams, J. S.; Bandler, S. R.; Betancourt-Martinez, G. L.; Chervenak, J. A.; Chiao, M. P.; Eckart, M. E.; Finkbeiner, F. M.; Kelley, R. L.; Kilbourne, C. A.; Miniussi, A. R.; Porter, F. S.; Sadleir, J. E.; Sakai, K.; Wakeham, N. A.; Wassell, E. J.; Yoon, W.; Bennett, D. A.; Doriese, W. B.; Fowler, J. W.; Hilton, G. C.; Morgan, K. M.; Pappas, C. G.; Reintsema, C. N.; Swetz, D. S.; Ullom, J. N.; Irwin, K. D.; Akamatsu, H.; Gottardi, L.; den Hartog, R.; Jackson, B. D.; van der Kuur, J.; Barret, D.; Peille, P.

    2016-07-01

    The focal plane of the X-ray integral field unit (X-IFU) for ESA's Athena X-ray observatory will consist of 4000 transition edge sensor (TES) x-ray microcalorimeters optimized for the energy range of 0.2 to 12 keV. The instrument will provide unprecedented spectral resolution of 2.5 eV at energies of up to 7 keV and will accommodate photon fluxes of 1 mCrab (90 cps) for point source observations. The baseline configuration is a uniform large pixel array (LPA) of 4.28" pixels that is read out using frequency domain multiplexing (FDM). However, an alternative configuration under study incorporates an 18 × 18 small pixel array (SPA) of 2" pixels in the central 36" region. This hybrid array configuration could be designed to accommodate higher fluxes of up to 10 mCrab (900 cps) or alternately for improved spectral performance (< 1.5 eV) at low count-rates. In this paper we report on the TES pixel designs that are being optimized to meet these proposed LPA and SPA configurations. In particular we describe details of how important TES parameters are chosen to meet the specific mission criteria such as energy resolution, count-rate and quantum efficiency, and highlight performance trade-offs between designs. The basis of the pixel parameter selection is discussed in the context of existing TES arrays that are being developed for solar and x-ray astronomy applications. We describe the latest results on DC biased diagnostic arrays as well as large format kilo-pixel arrays and discuss the technical challenges associated with integrating different array types on to a single detector die.

  12. Optimal Design of MPPT Controllers for Grid Connected Photovoltaic Array System

    Ebrahim, M. A.; AbdelHadi, H. A.; Mahmoud, H. M.; Saied, E. M.; Salama, M. M.

    2016-10-01

    Integrating photovoltaic (PV) plants into electric power system exhibits challenges to power system dynamic performance. These challenges stem primarily from the natural characteristics of PV plants, which differ in some respects from the conventional plants. The most significant challenge is how to extract and regulate the maximum power from the sun. This paper presents the optimal design for the most commonly used Maximum Power Point Tracking (MPPT) techniques based on Proportional Integral tuned by Particle Swarm Optimization (PI-PSO). These suggested techniques are, (1) the incremental conductance, (2) perturb and observe, (3) fractional short circuit current and (4) fractional open circuit voltage techniques. This research work provides a comprehensive comparative study with the energy availability ratio from photovoltaic panels. The simulation results proved that the proposed controllers have an impressive tracking response. The system dynamic performance improved greatly using the proposed controllers.

  13. Design of a muon tomography system with a plastic scintillator and wavelength-shifting fiber arrays

    Jo, Woo Jin; Kim, Hyun-Il; An, Su Jung; Lee, Chae Young [Department of Radiological Science, College of Health Science, Yonsei University, Wonju 220-710 (Korea, Republic of); Baek, Cheol-Ha [Department of Radiological Science, Dongseo University, Busan 617-716 (Korea, Republic of); Chung, Yong Hyun, E-mail: ychung@yonsei.ac.kr [Department of Radiological Science, College of Health Science, Yonsei University, Wonju 220-710 (Korea, Republic of)

    2013-12-21

    Recently, monitoring nuclear materials to avoid nuclear terrorism has become an important area of national security. It can be difficult to detect gamma rays from nuclear material because they are easily shielded by shielding material. Muon tomography using multiple -Coulomb scattering derived from muons can be utilized to detect special nuclear materials (SNMs) such as uranium-235 and plutonium-239. We designed a muon tomography system composed of four detector modules. The incident and scattered muon tracks can be calculated by two top and two bottom detectors, respectively. 3D tomographic images are obtained by extracting the crossing points of muon tracks with a point-of-closest-approach algorithm. The purpose of this study was to optimize the muon tomography system using Monte Carlo simulation code. The effects of the geometric parameters of the muon tomography system on material Z-discrimination capability were simulated and evaluated.

  14. A new method for the design of slot antenna arrays: Theory and experiment

    Clauzier, Sebastien

    2016-04-10

    The present paper proposes and validates a new general design methodology that can be used to automatically find proper positions and orientations of waveguide-based radiating slots capable of realizing any given radiation beam profile. The new technique combines basic radiation theory and waveguide propagation theory in a novel analytical model that allows the prediction of the radiation characteristics of generic slots without the need to perform full-wave numerical solution. The analytical model is then used to implement a low-cost objective function within a global optimization scheme (here genetic algorithm.) The algorithm is then deployed to find optimum positions and orientations of clusters of radiating slots cut into the waveguide surface such that any desired beam pattern can be obtained. The method is verified using both full-wave numerical solution and experiment.

  15. Calculation method of CGH for Binocular Eyepiece-Type Electro Holography

    Yang, Chanyoung; Yoneyama, Takuo; Sakamoto, Yuji; Okuyama, Fumio

    2013-01-01

    We had researched about eyepiece-type electro holography to display 3-D images of larger objects at wider angle. We had enlarged visual field considering depth of object with Fourier optical system using two lenses. In this paper, we extend our system for binocular. In the binocular system, we use two different holograms for each eye. The 3-D image for left eye should be observed like the real object observed using left eye and the same for right eye. So, we propose a method of calculation of computer-generated hologram (CGH) transforming the coordinate system of the model data to make two holograms for binocular eyepiece-type electro holography. The coordinate system of original model data is called the world coordinate system. The left and the right coordinate system are transformed from the world coordinate system. We also propose the method for correcting the installation error that occurs when placing the electronic and optical devices. The installation error is calculated and the model data is corrected using the distance between measured position and setup position of the reconstructed image Optical reconstruction experiments were carried out to verify the proposed method.

  16. Design and study of a coplanar grid array CdZnTe detector for improved spatial resolution

    Ma, Yuedong; Xiao, Shali; Yang, Guoqiang; Zhang, Liuqiang

    2014-01-01

    Coplanar grid (CPG) CdZnTe detectors have been used as gamma-ray spectrometers for years. Comparing with pixelated CdZnTe detectors, CPG CdZnTe detectors have either no or poor spatial resolution, which directly limits its use in imaging applications. To address the issue, a 2×2 CPG array CdZnTe detector with dimensions of 7×7×5 mm 3 was fabricated. Each of the CPG pairs in the detector was moderately shrunk in size and precisely designed to improve the spatial resolution while maintaining good energy resolution, considering the charge loss at the surface between the strips of each CPG pairs. Preliminary measurements were demonstrated at an energy resolution of 2.7–3.9% for the four CPG pairs using 662 keV gamma rays and with a spatial resolution of 3.3 mm, which is the best spatial resolution ever achieved for CPG CdZnTe detectors. The results reveal that the CPG CdZnTe detector can also be applied to imaging applications at a substantially higher spatial resolution. - Highlights: • A novel structure of coplanar grid CdZnTe detector was designed to evaluate the possibility of applying the detector to gamma-ray imaging applications. • The best spatial resolution of coplanar grid CdZnTe detectors ever reported has been achieved, along with good spectroscopic performance. • Depth correction of the energy spectra using a new algorithm is presented

  17. Design of a bovine low-density SNP array optimized for imputation.

    Didier Boichard

    Full Text Available The Illumina BovineLD BeadChip was designed to support imputation to higher density genotypes in dairy and beef breeds by including single-nucleotide polymorphisms (SNPs that had a high minor allele frequency as well as uniform spacing across the genome except at the ends of the chromosome where densities were increased. The chip also includes SNPs on the Y chromosome and mitochondrial DNA loci that are useful for determining subspecies classification and certain paternal and maternal breed lineages. The total number of SNPs was 6,909. Accuracy of imputation to Illumina BovineSNP50 genotypes using the BovineLD chip was over 97% for most dairy and beef populations. The BovineLD imputations were about 3 percentage points more accurate than those from the Illumina GoldenGate Bovine3K BeadChip across multiple populations. The improvement was greatest when neither parent was genotyped. The minor allele frequencies were similar across taurine beef and dairy breeds as was the proportion of SNPs that were polymorphic. The new BovineLD chip should facilitate low-cost genomic selection in taurine beef and dairy cattle.

  18. Design of collimating and rearrangement systems of laser diode array beam

    Gao, Runmei; Fang, Tao; Fu, Rulian; Yao, Jianquan

    2015-10-01

    To improve the laser diode output beam quality, micro-cylindrical lens and the step-type lens combination are designed. The former is used to collimate beam in fast-axis direction, while the latter plays a role in the slow-axis of splitting and the rearrangement. The micro-column semi-elliptical lens is made with the drops of spherical zoom lensin electric field and with the help of the material properties of light-cured production, which can reduce the reflection of the front surface and total reflection loss of the after. The divergence angle in the fast axis is compressed to roughly the same as that in the slow-axis direction; Stepped lens splits compressed long strip beam in the slow axis, with parallelogram style of level equidistant and rearrange in the fast axis direction. The spot in the slow axis gets smaller and the spot becomes larger in the fast axis. At last divergence angle and the beam spot achieve balanced in the fast axis and slow axis, optical parameters BPP integrates approximate the same, and beam quality can be improved.

  19. Field Programmable Gate Array Based Parallel Strapdown Algorithm Design for Strapdown Inertial Navigation Systems

    Long-Hua Ma

    2011-08-01

    Full Text Available A new generalized optimum strapdown algorithm with coning and sculling compensation is presented, in which the position, velocity and attitude updating operations are carried out based on the single-speed structure in which all computations are executed at a single updating rate that is sufficiently high to accurately account for high frequency angular rate and acceleration rectification effects. Different from existing algorithms, the updating rates of the coning and sculling compensations are unrelated with the number of the gyro incremental angle samples and the number of the accelerometer incremental velocity samples. When the output sampling rate of inertial sensors remains constant, this algorithm allows increasing the updating rate of the coning and sculling compensation, yet with more numbers of gyro incremental angle and accelerometer incremental velocity in order to improve the accuracy of system. Then, in order to implement the new strapdown algorithm in a single FPGA chip, the parallelization of the algorithm is designed and its computational complexity is analyzed. The performance of the proposed parallel strapdown algorithm is tested on the Xilinx ISE 12.3 software platform and the FPGA device XC6VLX550T hardware platform on the basis of some fighter data. It is shown that this parallel strapdown algorithm on the FPGA platform can greatly decrease the execution time of algorithm to meet the real-time and high precision requirements of system on the high dynamic environment, relative to the existing implemented on the DSP platform.

  20. Compression and radiation of high-power short rf pulses. II. A novel antenna array design with combined compressor/radiator elements

    Sirenko, Kostyantyn; Pazynin, Vadim L.; Sirenko, Yu K.; Bagci, Hakan

    2011-01-01

    The paper discusses the radiation of compressed high power short RF pulses using two different types of antennas: (i) A simple monopole antenna and (ii) a novel array design, where each of the elements is constructed by combining a compressor and a

  1. Report: Optimization study of the preparation factors for argan oil microcapsule based on hybrid-level orthogonal array design via SPSS modeling.

    Zhao, Xi; Wu, Xiaoli; Zhou, Hui; Jiang, Tao; Chen, Chun; Liu, Mingshi; Jin, Yuanbao; Yang, Dongsheng

    2014-11-01

    To optimize the preparation factors for argan oil microcapsule using complex coacervation of chitosan cross-linked with gelatin based on hybrid-level orthogonal array design via SPSS modeling. Eight relatively significant factors were firstly investigated and selected as calculative factors for the orthogonal array design from the total of ten factors effecting the preparation of argan oil microcapsule by utilizing the single factor variable method. The modeling of hybrid-level orthogonal array design was built in these eight factors with the relevant levels (9, 9, 9, 9, 7, 6, 2 and 2 respectively). The preparation factors for argan oil microcapsule were investigated and optimized according to the results of hybrid-level orthogonal array design. The priorities order and relevant optimum levels of preparation factors standard to base on the percentage of microcapsule with the diameter of 30~40 μm via SPSS. Experimental data showed that the optimum factors were controlling the chitosan/gelatin ratio, the systemic concentration and the core/shell ratio at 1:2, 1.5% and 1:7 respectively, presetting complex coacervation pH at 6.4, setting cross-linking time and complex coacervation at 75 min and 30 min, using the glucose-delta lactone as the type of cross-linking agent, and selecting chitosan with the molecular weight of 2000~3000.

  2. Concept and design of a genome-wide association genotyping array tailored for transplantation-specific studies

    Li, Yun R.; van Setten, Jessica; Verma, Shefali S.

    2015-01-01

    genome-wide genotyping array, the 'TxArray', comprising approximately 782,000 markers with tailored content for deeper capture of variants across HLA, KIR, pharmacogenomic, and metabolic loci important in transplantation. To test concordance and genotyping quality, we genotyped 85 HapMap samples...

  3. Design and implementation of a reconfigurable mixed-signal SoC based on field programmable analog arrays

    Liu, Lintao; Gao, Yuhan; Deng, Jun

    2017-11-01

    This work presents a reconfigurable mixed-signal system-on-chip (SoC), which integrates switched-capacitor-based field programmable analog arrays (FPAA), analog-to-digital converter (ADC), digital-to-analog converter, digital down converter , digital up converter, 32-bit reduced instruction-set computer central processing unit (CPU) and other digital IPs on a single chip with 0.18 μm CMOS technology. The FPAA intellectual property could be reconfigured as different function circuits, such as gain amplifier, divider, sine generator, and so on. This single-chip integrated mixed-signal system is a complete modern signal processing system, occupying a die area of 7 × 8 mm 2 and consuming 719 mW with a clock frequency of 150 MHz for CPU and 200 MHz for ADC/DAC. This SoC chip can help customers to shorten design cycles, save board area, reduce the system power consumption and depress the system integration risk, which would afford a big prospect of application for wireless communication. Project supported by the National High Technology and Development Program of China (No. 2012AA012303).

  4. Optimization of supercritical fluid extraction and HPLC identification of wedelolactone from Wedelia calendulacea by orthogonal array design.

    Patil, Ajit A; Sachin, Bhusari S; Wakte, Pravin S; Shinde, Devanand B

    2014-11-01

    The purpose of this work is to provide a complete study of the influence of operational parameters of the supercritical carbon dioxide assisted extraction (SC CO2E) on yield of wedelolactone from Wedelia calendulacea Less., and to find an optimal combination of factors that maximize the wedelolactone yield. In order to determine the optimal combination of the four factors viz. operating pressure, temperature, modifier concentration and extraction time, a Taguchi experimental design approach was used: four variables (three levels) in L9 orthogonal array. Wedelolactone content was determined using validated HPLC methodology. Optimum extraction conditions were found to be as follows: extraction pressure, 25 MPa; temperature, 40 °C; modifier concentration, 10% and extraction time, 90 min. Optimum extraction conditions demonstrated wedelolactone yield of 8.01 ± 0.34 mg/100 g W. calendulacea Less. Pressure, temperature and time showed significant (p yield. The supercritical carbon dioxide extraction showed higher selectivity than the conventional Soxhlet assisted extraction method.

  5. Optimization of supercritical fluid extraction and HPLC identification of wedelolactone from Wedelia calendulacea by orthogonal array design

    Ajit A. Patil

    2014-11-01

    Full Text Available The purpose of this work is to provide a complete study of the influence of operational parameters of the supercritical carbon dioxide assisted extraction (SC CO2E on yield of wedelolactone from Wedelia calendulacea Less., and to find an optimal combination of factors that maximize the wedelolactone yield. In order to determine the optimal combination of the four factors viz. operating pressure, temperature, modifier concentration and extraction time, a Taguchi experimental design approach was used: four variables (three levels in L9 orthogonal array. Wedelolactone content was determined using validated HPLC methodology. Optimum extraction conditions were found to be as follows: extraction pressure, 25 MPa; temperature, 40 °C; modifier concentration, 10% and extraction time, 90 min. Optimum extraction conditions demonstrated wedelolactone yield of 8.01 ± 0.34 mg/100 g W. calendulacea Less. Pressure, temperature and time showed significant (p < 0.05 effect on the wedelolactone yield. The supercritical carbon dioxide extraction showed higher selectivity than the conventional Soxhlet assisted extraction method.

  6. [Analysis of clinical outcomes of different embryo stage biopsy in array comparative genomic hybridization based preimplantation genetic diagnosis and screening].

    Shen, J D; Wu, W; Shu, L; Cai, L L; Xie, J Z; Ma, L; Sun, X P; Cui, Y G; Liu, J Y

    2017-12-25

    Objective: To evaluate the efficiency of the application of array comparative genomic hybridization (array-CGH) in preimplantation genetic diagnosis or screening (PGD/PGS), and compare the clinical outcomes of different stage embryo biopsy. Methods: The outcomes of 381 PGD/PGS cycles referred in the First Affiliated Hospital of Nanjing Medical University from July 2011 to August 2015 were retrospectively analyzed. There were 320 PGD cycles with 156 cleavage-stage-biopsy cycles and 164 trophectoderm-biopsy cycles, 61 PGS cycles with 23 cleavage-stage-biopsy cycles and 38 trophectoderm-biopsy cycles. Chromosomal analysis was performed by array-CGH technology combined with whole genome amplification. Single embryo transfer was performed in all transfer cycles. Live birth rate was calculated as the main clinical outcomes. Results: The embryo diagnosis rate of PGD/PGS by array-CGH were 96.9%-99.1%. In PGD biopsy cycles, the live birth rate per embryo transfer cycle and live birth rate per embryo biopsy cycle were 50.0%(58/116) and 37.2%(58/156) in cleavage-stage-biopsy group, 67.5%(85/126) and 51.8%(85/164) in trophectoderm-biopsy group (both P 0.05). Conclusions: High diagnosis rate and idea live birth rate are achieved in PGD/PGS cycles based on array-CGH technology. The live birth rate of trophectoderm-biopsy group is significantly higher than that of cleavage-stage-biopsy group in PGD cycles; the efficiency of trophectoderm-biopsy is better.

  7. Conceptual design and optimization of a plastic scintillator array for 2D tomography using a compact D-D fast neutron generator.

    Adams, Robert; Zboray, Robert; Cortesi, Marco; Prasser, Horst-Michael

    2014-04-01

    A conceptual design optimization of a fast neutron tomography system was performed. The system is based on a compact deuterium-deuterium fast neutron generator and an arc-shaped array of individual neutron detectors. The array functions as a position sensitive one-dimensional detector allowing tomographic reconstruction of a two-dimensional cross section of an object up to 10 cm across. Each individual detector is to be optically isolated and consists of a plastic scintillator and a Silicon Photomultiplier for measuring light produced by recoil protons. A deterministic geometry-based model and a series of Monte Carlo simulations were used to optimize the design geometry parameters affecting the reconstructed image resolution. From this, it is expected that with an array of 100 detectors a reconstructed image resolution of ~1.5mm can be obtained. Other simulations were performed in order to optimize the scintillator depth (length along the neutron path) such that the best ratio of direct to scattered neutron counts is achieved. This resulted in a depth of 6-8 cm and an expected detection efficiency of 33-37%. Based on current operational capabilities of a prototype neutron generator being developed at the Paul Scherrer Institute, planned implementation of this detector array design should allow reconstructed tomograms to be obtained with exposure times on the order of a few hours. Copyright © 2014 Elsevier Ltd. All rights reserved.

  8. Design of a rectenna system for GSM-900 band using novel broadside 2 × 1 array antenna

    Manish Singh

    2017-05-01

    Full Text Available In this study, a rectenna operating at the GSM-900 frequency band has been fabricated and tested. This rectenna composed of a 2 × 1 T-shaped monopole array antenna and an energy processing circuit. In order to reduce the gap between adjacent antenna elements in the array structure, the proposed array antenna uses a ground stub. Compared with other array antennas, the proposed array antenna with the ground stub reduces the size up to 50% without affecting the gain and bandwidth. An antenna prototype is fabricated and experimentally tested. The measured antenna's gain and bandwidth are 3.2 and 152 MHz, respectively, hence showing its suitability for radio-frequency (RF energy harvesting application. For this to be feasible, the developed array antenna is matched with the rectifier at GSM-900 using a single stub matching network. The measured result demonstrates that the proposed rectifier circuit offers the conversion efficiency of 21.2 and 63.6% for an input power of −20 and 0 dBm, respectively. Finally, the rectifier performance is attested experimentally with the developed array antenna. The rectenna's measured RF-to-dc conversion efficiency was found to be 60% at the far-field distance from the transmitting antenna.

  9. High performance architecture design for large scale fibre-optic sensor arrays using distributed EDFAs and hybrid TDM/DWDM

    Liao, Yi; Austin, Ed; Nash, Philip J.; Kingsley, Stuart A.; Richardson, David J.

    2013-09-01

    A distributed amplified dense wavelength division multiplexing (DWDM) array architecture is presented for interferometric fibre-optic sensor array systems. This architecture employs a distributed erbium-doped fibre amplifier (EDFA) scheme to decrease the array insertion loss, and employs time division multiplexing (TDM) at each wavelength to increase the number of sensors that can be supported. The first experimental demonstration of this system is reported including results which show the potential for multiplexing and interrogating up to 4096 sensors using a single telemetry fibre pair with good system performance. The number can be increased to 8192 by using dual pump sources.

  10. A Two-dimensional Sixteen Channel Transmit/Receive Coil Array for Cardiac MRI at 7.0 Tesla: Design, Evaluation and Application

    Thalhammer, Christof; Renz, Wolfgang; Winter, Lukas; Hezel, Fabian; Rieger, Jan; Pfeiffer, Harald; Graessl, Andreas; Seifert, Frank; Hoffmann, Werner; von Knobelsdorff-Brenkenhoff, Florian; Tkachenko, Valeriy; Schulz-Menger, Jeanette; Kellman, Peter; Niendorf, Thoralf

    2012-01-01

    Purpose To design, evaluate and apply a two-dimensional 16 channel transmit/receive coil array tailored for cardiac MRI at 7.0 Tesla. Material and Methods The cardiac coil array consists of 2 sections each using 8 elements arranged in a 2 × 4 array. RF safety was validated by SAR simulations. Cardiac imaging was performed using 2D CINE FLASH imaging, T2* mapping and fat-water separation imaging. The characteristics of the coil array were analyzed including parallel imaging performance, left ventricular chamber quantification and overall image quality. Results RF characteristics were found to be appropriate for all subjects included in the study. The SAR values derived from the simulations fall well in the limits of legal guidelines. The baseline SNR advantage at 7.0 T was put to use to acquire 2D CINE images of the heart with a very high spatial resolution of (1 × 1 × 4) mm3. The proposed coil array supports 1D acceleration factors of up to R=4 without impairing image quality significantly. Conclusions The 16 channel TX/RX coil has the capability to acquire high contrast and high spatial resolution images of the heart at 7.0 Tesla. PMID:22706727

  11. An investigation of signal performance enhancements achieved through innovative pixel design across several generations of indirect detection, active matrix, flat-panel arrays

    Antonuk, Larry E.; Zhao Qihua; El-Mohri, Youcef; Du Hong; Wang Yi; Street, Robert A.; Ho, Jackson; Weisfield, Richard; Yao, William

    2009-01-01

    Active matrix flat-panel imager (AMFPI) technology is being employed for an increasing variety of imaging applications. An important element in the adoption of this technology has been significant ongoing improvements in optical signal collection achieved through innovations in indirect detection array pixel design. Such improvements have a particularly beneficial effect on performance in applications involving low exposures and/or high spatial frequencies, where detective quantum efficiency is strongly reduced due to the relatively high level of additive electronic noise compared to signal levels of AMFPI devices. In this article, an examination of various signal properties, as determined through measurements and calculations related to novel array designs, is reported in the context of the evolution of AMFPI pixel design. For these studies, dark, optical, and radiation signal measurements were performed on prototype imagers incorporating a variety of increasingly sophisticated array designs, with pixel pitches ranging from 75 to 127 μm. For each design, detailed measurements of fundamental pixel-level properties conducted under radiographic and fluoroscopic operating conditions are reported and the results are compared. A series of 127 μm pitch arrays employing discrete photodiodes culminated in a novel design providing an optical fill factor of ∼80% (thereby assuring improved x-ray sensitivity), and demonstrating low dark current, very low charge trapping and charge release, and a large range of linear signal response. In two of the designs having 75 and 90 μm pitches, a novel continuous photodiode structure was found to provide fill factors that approach the theoretical maximum of 100%. Both sets of novel designs achieved large fill factors by employing architectures in which some, or all of the photodiode structure was elevated above the plane of the pixel addressing transistor. Generally, enhancement of the fill factor in either discrete or continuous

  12. Multi-frequency response from a designed array of micromechanical cantilevers fabricated using a focused ion beam

    Ghatnekar-Nilsson, S; Graham, J; Hull, R; Montelius, L

    2006-01-01

    We demonstrate arrays of cantilevers with different lengths, fabricated by focused ion beam milling. The arrays of oscillators generate a spectrum of different resonant frequencies, where each frequency correlates to the corresponding individual cantilever. The frequency response from all the cantilevers is collected from a single measurement under the same environment and conditions for the entire array. The mass response of the system generated the same Δf/f 0 for the cantilevers, within 0.1% accuracy. We denote the method MFSAC: multi-frequency signal analysis from an array of cantilevers. The simultaneous detection of several frequencies in one spectrum has great benefits in mass sensor applications, offering the possibility for true label-free detection

  13. Design of a QA method to characterize submillimeter-sized PBS beam properties using a 2D ionization chamber array

    Lin, Yuting; Bentefour, Hassan; Flanz, Jacob; Kooy, Hanne; Clasie, Benjamin

    2018-05-01

    Pencil beam scanning (PBS) periodic quality assurance (QA) programs ensure the beam delivered to patients is within technical specifications. Two critical specifications for PBS delivery are the beam width and position. The aim of this study is to investigate whether a 2D ionization chamber array, such as the MatriXX detector (IBA Dosimetry, Schwarzenbruck, Germany), can be used to characterize submillimeter-sized PBS beam properties. The motivation is to use standard equipment, which may have pixel spacing coarser than the pencil beam size, and simplify QA workflow. The MatriXX pixels are cylindrical in shape with 4.5 mm diameter and are spaced 7.62 mm from center to center. Two major effects limit the ability of using the MatriXX to measure the spot position and width accurately. The first effect is that too few pixels sample the Gaussian shaped pencil beam profile and the second effect is volume averaging of the Gaussian profile over the pixel sensitive volumes. We designed a method that overcomes both limitations and hence enables the use of the MatriXX to characterize sub-millimeter-sized PBS beam properties. This method uses a cross-like irradiation pattern that is designed to increase the number of sampling data points and a modified Gaussian fitting technique to correct for volume averaging effects. Detector signals were calculated in this study and random noise and setup errors were added to simulate measured data. With the techniques developed in this work, the MatriXX detector can be used to characterize the position and width of sub-millimeter, σ  =  0.7 mm, sized pencil beams with uncertainty better than 3% relative to σ. With the irradiation only covering 60% of the MatriXX, the position and width of σ  =  0.9 mm sized pencil beams can be determined with uncertainty better than 3% relative to σ. If one were to not use a cross-like irradiation pattern, then the position and width of σ  =  3.6 mm sized pencil beams

  14. Analysis of genomic alterations in neuroblastoma by multiplex ligation-dependent probe amplification and array comparative genomic hybridization: a comparison of results.

    Combaret, Valérie; Iacono, Isabelle; Bréjon, Stéphanie; Schleiermacher, Gudrun; Pierron, Gäelle; Couturier, Jérôme; Bergeron, Christophe; Blay, Jean-Yves

    2012-12-01

    In cases of neuroblastoma, recurring genetic alterations--losses of the 1p, 3p, 4p, and 11q and/or gains of 1q, 2p, and 17q chromosome arms--are currently used to define the therapeutic strategy in therapeutic protocols for low- and intermediate-risk patients. Different genome-wide analysis techniques, such as array comparative genomic hybridization (aCGH) or multiplex ligation-dependent probe amplification (MLPA), have been suggested for detecting chromosome segmental abnormalities. In this study, we compared the results of the two technologies in the analyses of the DNA of tumor samples from 91 neuroblastoma patients. Similar results were obtained with the two techniques for 75 samples (82%). In five cases (5.5%), the MLPA results were not interpretable. Discrepancies between the aCGH and MLPA results were observed in 11 cases (12%). Among the discrepancies, a 18q21.2-qter gain and 16p11.2 and 11q14.1-q14.3 losses were detected only by aCGH. The MLPA results showed that the 7p, 7q, and 14q chromosome arms were affected in six cases, while in two cases, 2p and 17q gains were observed; these results were confirmed by neither aCGH nor fluorescence in situ hybridization (FISH) analysis. Because of the higher sensitivity and specificity of genome-wide information, reasonable cost, and shorter time of aCGH analysis, we recommend the aCGH procedure for the analysis of genomic alterations in neuroblastoma. Copyright © 2012 Elsevier Inc. All rights reserved.

  15. Design and performance of large-pixel-size high-fill-fraction TES arrays for future X-ray astrophysics missions

    Figueroa-Feliciano, E.; Bandler, S.R.; Chervenak, J.; Finkbeiner, F.; Iyomoto, N.; Kelley, R.L.; Kilbourne, C.A.; Porter, F.S.; Saab, T.; Sadleir, J.; White, J.

    2006-01-01

    We have designed, modeled, fabricated and tested a 600μm high-fill-fraction microcalorimeter array that will be a good match to the requirements of future X-ray missions. Our devices use transition-edge sensors coupled to overhanging bismuth/copper absorbers to produce arrays with 97% or higher fill fraction. An extensive modeling effort was undertaken in order to accommodate large pixel sizes (500-1000μm) and maintain the best energy resolution possible. The finite thermalization time of the large absorber and the associated position dependence of the pulse shape on absorption position constrain the time constants of the system given a desired energy-resolution performance. We show the results of our analysis and our new pixel design, consisting of a novel TES-on-the-side architecture which creates a controllable TES-absorber conductance

  16. Reliability of mechanical and phased-array designs for serial intravascular ultrasound examinations--animal and clinical studies in stented and non-stented coronary arteries.

    Tardif, J C; Bertrand, O F; Mongrain, R; Lespérance, J; Grégoire, J; Paiement, P; Bonan, R

    2000-10-01

    Both mechanical and multi-element intravascular ultrasound designs have potential advantages and limitations that may impact on their value for clinical and research purposes. Determination of the reproducibility of measurements is critical before a given system can be used in studies such as regression of atherosclerosis trials. We performed serial intravascular ultrasound imaging with catheters using mechanical and phased-array designs in stented and non-stented coronary arteries in dogs and in patients. Both systems correlated well for areas (r > or = 0.90, p or = 0.84. p mechanical designs for measurements of area (mean difference in dogs and in patients: -0.24 and 0.96 mm2, p mechanical system (r > or = 0.96 for all measurements). The differences in absolute and relative variability between the mechanical and phased-array designs, both for reanalysis of same frames and serial pullbacks, were very small. Although multi-element and mechanical intravascular ultrasound designs are not strictly interchangeable, their similar reproducibility and the small differences in measurements demonstrate that both designs are acceptable alternatives for trials of regression of atherosclerosis. Determination of the variability for serial pullbacks of both designs was also important to assess the statistical power of such trials.

  17. NF2 tumor suppressor gene: a comprehensive and efficient detection of somatic mutations by denaturing HPLC and microarray-CGH.

    Szijan, Irene; Rochefort, Daniel; Bruder, Carl; Surace, Ezequiel; Machiavelli, Gloria; Dalamon, Viviana; Cotignola, Javier; Ferreiro, Veronica; Campero, Alvaro; Basso, Armando; Dumanski, Jan P; Rouleau, Guy A

    2003-01-01

    The NF2 tumor suppressor gene, located in chromosome 22q12, is involved in the development of multiple tumors of the nervous system, either associated with neurofibromatosis 2 or sporadic ones, mainly schwannomas and meningiomas. In order to evaluate the role of the NF2 gene in sporadic central nervous system (CNS) tumors, we analyzed NF2 mutations in 26 specimens: 14 meningiomas, 4 schwannomas, 4 metastases, and 4 other histopathological types of neoplasms. Denaturing high performance liquid chromatography (denaturing HPLC) and comparative genomic hybridization on a DNA microarray (microarray- CGH) were used as scanning methods for small mutations and gross rearrangements respectively. Small mutations were identified in six out of seventeen meningiomas and schwannomas, one mutation was novel. Large deletions were detected in six meningiomas. All mutations were predicted to result in truncated protein or in the absence of a large protein domain. No NF2 mutations were found in other histopathological types of CNS tumors. These results provide additional evidence that mutations in the NF2 gene play an important role in the development of sporadic meningiomas and schwannomas. Denaturing HPLC analysis of small mutations and microarray-CGH of large deletions are complementary, fast, and efficient methods for the detection of mutations in tumor tissues.

  18. Design of Vivaldi antenna array with end-fire beam steering function for 5G mobile terminals

    Ojaroudiparchin, Naser; Shen, Ming; Pedersen, Gert F.

    2015-01-01

    to form a linear phased array in the edge region (top-side) on a mobile phone PCB. The simulated results show that the antenna has the reflection coefficient (S11) less than -10 dB in the frequency range of 27.4 to 28.6 GHz. The proposed phased array antenna has good gain, efficiency, and 3D beam steering...... characteristics in the entire operation band, which makes it suitable for millimeter-wave 5G communications. In addition, the performance of the antenna in the vicinity of user’s hand has been investigated in this study....

  19. Peptide array-based interaction assay of solid-bound peptides and anchorage-dependant cells and its effectiveness in cell-adhesive peptide design.

    Kato, Ryuji; Kaga, Chiaki; Kunimatsu, Mitoshi; Kobayashi, Takeshi; Honda, Hiroyuki

    2006-06-01

    Peptide array, the designable peptide library covalently synthesized on cellulose support, was applied to assay peptide-cell interaction, between solid-bound peptides and anchorage-dependant cells, to study objective peptide design. As a model case, cell-adhesive peptides that could enhance cell growth as tissue engineering scaffold material, was studied. On the peptide array, the relative cell-adhesion ratio of NIH/3T3 cells was 2.5-fold higher on the RGDS (Arg-Gly-Asp-Ser) peptide spot as compared to the spot with no peptide, thus indicating integrin-mediated peptide-cell interaction. Such strong cell adhesion mediated by the RGDS peptide was easily disrupted by single residue substitution on the peptide array, thus indicating that the sequence recognition accuracy of cells was strictly conserved in our optimized scheme. The observed cellular morphological extension with active actin stress-fiber on the RGD motif-containing peptide supported our strategy that peptide array-based interaction assay of solid-bound peptide and anchorage-dependant cells (PIASPAC) could provide quantitative data on biological peptide-cell interaction. The analysis of 180 peptides obtained from fibronectin type III domain (no. 1447-1629) yielded 18 novel cell-adhesive peptides without the RGD motif. Taken together with the novel candidates, representative rules of ineffective amino acid usage were obtained from non-effective candidate sequences for the effective designing of cell-adhesive peptides. On comparing the amino acid usage of the top 20 and last 20 peptides from the 180 peptides, the following four brief design rules were indicated: (i) Arg or Lys of positively charged amino acids (except His) could enhance cell adhesion, (ii) small hydrophilic amino acids are favored in cell-adhesion peptides, (iii) negatively charged amino acids and small amino acids (except Gly) could reduce cell adhesion, and (iv) Cys and Met could be excluded from the sequence combination since they have

  20. Novel ring resonator-based integrated photonic beamformer for broadband phased array receive antennas - part 1: design and performance analysis

    Meijerink, Arjan; Roeloffzen, C.G.H.; Meijerink, Roland; Zhuang, L.; Marpaung, D.A.I.; Bentum, Marinus Jan; Burla, M.; Verpoorte, Jaco; Jorna, Pieter; Huizinga, Adriaan; van Etten, Wim

    2010-01-01

    A novel optical beamformer concept is introduced that can be used for seamless control of the reception angle in broadband wireless receivers employing a large phased array antenna (PAA). The core of this beamformer is an optical beamforming network (OBFN), using ring resonator-based broadband

  1. Rational Design of QCM-D Virtual Sensor Arrays Based on Film Thickness, Viscoelasticity, and Harmonics for Vapor Discrimination.

    Speller, Nicholas C; Siraj, Noureen; Regmi, Bishnu P; Marzoughi, Hassan; Neal, Courtney; Warner, Isiah M

    2015-01-01

    Herein, we demonstrate an alternative strategy for creating QCM-based sensor arrays by use of a single sensor to provide multiple responses per analyte. The sensor, which simulates a virtual sensor array (VSA), was developed by depositing a thin film of ionic liquid, either 1-octyl-3-methylimidazolium bromide ([OMIm][Br]) or 1-octyl-3-methylimidazolium thiocyanate ([OMIm][SCN]), onto the surface of a QCM-D transducer. The sensor was exposed to 18 different organic vapors (alcohols, hydrocarbons, chlorohydrocarbons, nitriles) belonging to the same or different homologous series. The resulting frequency shifts (Δf) were measured at multiple harmonics and evaluated using principal component analysis (PCA) and discriminant analysis (DA) which revealed that analytes can be classified with extremely high accuracy. In almost all cases, the accuracy for identification of a member of the same class, that is, intraclass discrimination, was 100% as determined by use of quadratic discriminant analysis (QDA). Impressively, some VSAs allowed classification of all 18 analytes tested with nearly 100% accuracy. Such results underscore the importance of utilizing lesser exploited properties that influence signal transduction. Overall, these results demonstrate excellent potential of the virtual sensor array strategy for detection and discrimination of vapor phase analytes utilizing the QCM. To the best of our knowledge, this is the first report on QCM VSAs, as well as an experimental sensor array, that is based primarily on viscoelasticity, film thickness, and harmonics.

  2. Timed arrays wideband and time varying antenna arrays

    Haupt, Randy L

    2015-01-01

    Introduces timed arrays and design approaches to meet the new high performance standards The author concentrates on any aspect of an antenna array that must be viewed from a time perspective. The first chapters briefly introduce antenna arrays and explain the difference between phased and timed arrays. Since timed arrays are designed for realistic time-varying signals and scenarios, the book also reviews wideband signals, baseband and passband RF signals, polarization and signal bandwidth. Other topics covered include time domain, mutual coupling, wideband elements, and dispersion. The auth

  3. Analyses of Genotypes and Phenotypes of Ten Chinese Patients with Wolf-Hirschhorn Syndrome by Multiplex Ligation-dependent Probe Amplification and Array Comparative Genomic Hybridization.

    Yang, Wen-Xu; Pan, Hong; Li, Lin; Wu, Hai-Rong; Wang, Song-Tao; Bao, Xin-Hua; Jiang, Yu-Wu; Qi, Yu

    2016-03-20

    Wolf-Hirschhorn syndrome (WHS) is a contiguous gene syndrome that is typically caused by a deletion of the distal portion of the short arm of chromosome 4. However, there are few reports about the features of Chinese WHS patients. This study aimed to characterize the clinical and molecular cytogenetic features of Chinese WHS patients using the combination of multiplex ligation-dependent probe amplification (MLPA) and array comparative genomic hybridization (array CGH). Clinical information was collected from ten patients with WHS. Genomic DNA was extracted from the peripheral blood of the patients. The deletions were analyzed by MLPA and array CGH. All patients exhibited the core clinical symptoms of WHS, including severe growth delay, a Greek warrior helmet facial appearance, differing degrees of intellectual disability, and epilepsy or electroencephalogram anomalies. The 4p deletions ranged from 2.62 Mb to 17.25 Mb in size and included LETM1, WHSC1, and FGFR3. The combined use of MLPA and array CGH is an effective and specific means to diagnose WHS and allows for the precise identification of the breakpoints and sizes of deletions. The deletion of genes in the WHS candidate region is closely correlated with the core WHS phenotype.

  4. Design of patient-specific focused ultrasound arrays for non-invasive brain therapy with increased trans-skull transmission and steering range

    Hughes, Alec; Hynynen, Kullervo

    2017-09-01

    The use of a phased array of ultrasound transducer elements to sonicate through the skull has opened the way for new treatments and the delivery of therapeutics beyond the blood-brain barrier. The limited steering range of current clinical devices, particularly at higher frequencies, limits the regions of the brain that are considered treatable by ultrasound. A new array design is introduced that allows for high levels of beam steering and increased transmission throughout the brain. These improvements are achieved using concave transducers normal to the outer-skull surface in a patient-specific configuration to target within the skull, so that the far-field of each beam is within the brain. It is shown that by using pulsed ultrasound waves timed to arrive in-phase at the desired target, sufficient levels of acoustic energy are delivered for blood-brain barrier opening throughout the brain.

  5. Software design and code generation for the engineering graphical user interface of the ASTRI SST-2M prototype for the Cherenkov Telescope Array

    Tanci, Claudio; Tosti, Gino; Antolini, Elisa; Gambini, Giorgio F.; Bruno, Pietro; Canestrari, Rodolfo; Conforti, Vito; Lombardi, Saverio; Russo, Federico; Sangiorgi, Pierluca; Scuderi, Salvatore

    2016-08-01

    ASTRI is an on-going project developed in the framework of the Cherenkov Telescope Array (CTA). An end- to-end prototype of a dual-mirror small-size telescope (SST-2M) has been installed at the INAF observing station on Mt. Etna, Italy. The next step is the development of the ASTRI mini-array composed of nine ASTRI SST-2M telescopes proposed to be installed at the CTA southern site. The ASTRI mini-array is a collaborative and international effort carried on by Italy, Brazil and South-Africa and led by the Italian National Institute of Astrophysics, INAF. To control the ASTRI telescopes, a specific ASTRI Mini-Array Software System (MASS) was designed using a scalable and distributed architecture to monitor all the hardware devices for the telescopes. Using code generation we built automatically from the ASTRI Interface Control Documents a set of communication libraries and extensive Graphical User Interfaces that provide full access to the capabilities offered by the telescope hardware subsystems for testing and maintenance. Leveraging these generated libraries and components we then implemented a human designed, integrated, Engineering GUI for MASS to perform the verification of the whole prototype and test shared services such as the alarms, configurations, control systems, and scientific on-line outcomes. In our experience the use of code generation dramatically reduced the amount of effort in development, integration and testing of the more basic software components and resulted in a fast software release life cycle. This approach could be valuable for the whole CTA project, characterized by a large diversity of hardware components.

  6. Co-Prime Frequency and Aperture Design for HF Surveillance, Wideband Radar Imaging, and Nonstationary Array Processing

    2018-03-10

    circuit boards. A computational electromagnetics software package, FEKO [24], is used to model the antenna arrays, and the RMIM [12] is used to...Symposium on Intelligent Signal Processing and Communications Systems, Chengdu, China, 2010. [24] FEKO Suite 6.3, EM Software & Systems- S.A. (Pty) Ltd...including suggestions for reducing the burden, to Department of Defense, Washington Headquarters Services , Directorate for Information Operations and

  7. Comparative genomic hybridization (CGH) analysis of stage 4 neuroblastoma reveals high frequency of 11q deletion in tumors lacking MYCN amplification

    Plantaz, D.; Vandesompele, J.; van Roy, N.; Lastowska, M.; Bown, N.; Combaret, V.; Favrot, M. C.; Delattre, O.; Michon, J.; Bénard, J.; Hartmann, O.; Nicholson, J. C.; Ross, F. M.; Brinkschmidt, C.; Laureys, G.; Caron, H.; Matthay, K. K.; Feuerstein, B. G.; Speleman, F.

    2001-01-01

    We have studied the occurrence and association of 11q deletions with other chromosomal imbalances in Stage 4 neuroblastomas. To this purpose we have performed comparative genomic hybridization (CGH) analysis on 50 Stage 4 neuroblastomas and these data were analyzed together with those from 33

  8. Design, simulation and characterization of a MEMS inertia switch with flexible CNTs/Cu composite array layer between electrodes for prolonging contact time

    Wang, Yang; Yang, Zhuoqing; Xu, Qiu; Chen, Wenguo; Ding, Guifu; Zhao, Xiaolin

    2015-01-01

    This paper reports an inertia switch with a flexible carbon nanotubes/copper (CNTs/Cu) composite array layer between movable and fixed electrodes, which achieves a longer contact time compared to the traditional design using rigid-to-rigid impact between electrodes. The CNTs/Cu layer is fabricated using the composite electroplating method, and the whole device is completed by multi-layer metal electroplating based on the micro-electro-mechanical systems (MEMS) process. The dynamic responses of the designed inertia switch and the contact impact between a single CNT and a fixed electrode/another CNT have both been simulated by the ANSYS finite-element-method (FEM). It is shown that the contact time of the designed inertia switch is about 100 µs under the applied 80 g half-sine-shaped acceleration in the sensing direction. Finally, the fabricated MEMS inertia switch with the flexible CNTs/Cu composite array layer between electrodes has been evaluated by a dropping hammer system. The test contact time is about112 µs, which has a good agreement with the simulation and is much longer than that of the traditional design. (paper)

  9. “Distributed hybrid” MH–CGH2 system for hydrogen storage and its supply to LT PEMFC power modules

    Lototskyy, M., E-mail: mlototskyy@uwc.ac.za [HySA Systems Competence Centre, South African Institute for Advanced Materials Chemistry, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville 7535 (South Africa); Tolj, I.; Davids, M.W.; Bujlo, P. [HySA Systems Competence Centre, South African Institute for Advanced Materials Chemistry, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville 7535 (South Africa); Smith, F. [Impala Platinum Ltd, Springs (South Africa); Pollet, B.G. [HySA Systems Competence Centre, South African Institute for Advanced Materials Chemistry, Faculty of Natural Sciences, University of the Western Cape, Private Bag X17, Bellville 7535 (South Africa)

    2015-10-05

    Highlights: • Prototype hydrogen storage and supply system for LTPEMFC applications was developed. • Combination of MH and CGH2 tanks with common gas manifold was used. • Thermal coupling of fuel cell stack and MH tank was applied. • The system uses AB2-type MH; H2 equilibrium pressure ∼10 bar at room temperature. • Shorter H2 charge time and stable H2 supply at a fluctuating load were observed. - Abstract: This paper describes the layout and presents the results of the testing of a novel prototype “distributed hybrid” hydrogen storage and supply system that has the potential to be used for Low Temperature Proton Exchange Membrane Fuel Cell (LT-PEMFC) applications. The system consists of individual Metal Hydride (MH) and Compressed Gas (CGH2) tanks with common gas manifold, and a thermal management system where heat exchanger of the liquid heated-cooled MH tank is integrated with the cooling system of the LT-PEMFC BoP. The MH tank is filled with a medium-stability AB{sub 2}-type MH material (H{sub 2} equilibrium pressure of about 10 bar at room temperature). This innovative solution allows for (i) an increase in hydrogen storage capacity of the whole gas storage system and the reduction of H{sub 2} charge pressure; (ii) shorter charging times in the refuelling mode and smoother peaks of H{sub 2} consumption during its supply to the fuel cell stack; (iii) the use of standard parts with simple layout and lower costs; and (iv) adding flexibility in the layout and placement of the components of the hydrogen storage and supply system.

  10. “Distributed hybrid” MH–CGH2 system for hydrogen storage and its supply to LT PEMFC power modules

    Lototskyy, M.; Tolj, I.; Davids, M.W.; Bujlo, P.; Smith, F.; Pollet, B.G.

    2015-01-01

    Highlights: • Prototype hydrogen storage and supply system for LTPEMFC applications was developed. • Combination of MH and CGH2 tanks with common gas manifold was used. • Thermal coupling of fuel cell stack and MH tank was applied. • The system uses AB2-type MH; H2 equilibrium pressure ∼10 bar at room temperature. • Shorter H2 charge time and stable H2 supply at a fluctuating load were observed. - Abstract: This paper describes the layout and presents the results of the testing of a novel prototype “distributed hybrid” hydrogen storage and supply system that has the potential to be used for Low Temperature Proton Exchange Membrane Fuel Cell (LT-PEMFC) applications. The system consists of individual Metal Hydride (MH) and Compressed Gas (CGH2) tanks with common gas manifold, and a thermal management system where heat exchanger of the liquid heated-cooled MH tank is integrated with the cooling system of the LT-PEMFC BoP. The MH tank is filled with a medium-stability AB 2 -type MH material (H 2 equilibrium pressure of about 10 bar at room temperature). This innovative solution allows for (i) an increase in hydrogen storage capacity of the whole gas storage system and the reduction of H 2 charge pressure; (ii) shorter charging times in the refuelling mode and smoother peaks of H 2 consumption during its supply to the fuel cell stack; (iii) the use of standard parts with simple layout and lower costs; and (iv) adding flexibility in the layout and placement of the components of the hydrogen storage and supply system

  11. β-galactosidase Production by Aspergillus niger ATCC 9142 Using Inexpensive Substrates in Solid-State Fermentation: Optimization by Orthogonal Arrays Design.

    Kazemi, Samaneh; Khayati, Gholam; Faezi-Ghasemi, Mohammad

    2016-01-01

    Enzymatic hydrolysis of lactose is one of the most important biotechnological processes in the food industry, which is accomplished by enzyme β-galactosidase (β-gal, β-D-galactoside galactohydrolase, EC 3.2.1.23), trivial called lactase. Orthogonal arrays design is an appropriate option for the optimization of biotechnological processes for the production of microbial enzymes. Design of experimental (DOE) methodology using Taguchi orthogonal array (OA) was employed to screen the most significant levels of parameters, including the solid substrates (wheat straw, rice straw, and peanut pod), the carbon/nitrogen (C/N) ratios, the incubation time, and the inducer. The level of β-gal production was measured by a photometric enzyme activity assay using the artificial substrate ortho-Nitrophenyl-β-D-galactopyranoside. The results showed that C/N ratio (0.2% [w/v], incubation time (144 hour), and solid substrate (wheat straw) were the best conditions determined by the design of experiments using the Taguchi approach. Our finding showed that the use of rice straw and peanut pod, as solid-state substrates, led to 2.041-folds increase in the production of the enzyme, as compared to rice straw. In addition, the presence of an inducer did not have any significant impact on the enzyme production levels.

  12. Design and Analysis of Printed Yagi-Uda Antenna and Two-Element Array for WLAN Applications

    Cai Run-Nan

    2012-01-01

    Full Text Available A printed director antenna with compact structure is proposed. The antenna is fed by a balanced microstrip-slotline and makes good use of space to reduce feeding network area and the size of antenna. According to the simulation results of CST MICROWAVE STUDIO software, broadband characteristics and directional radiation properties of the antenna are explained. The operating bandwidth is 1.8 GHz–3.5 GHz with reflection coefficient less than −10 dB. Antenna gain in band can achieve 4.5–6.8 dBi, and the overall size of antenna is smaller than 0.34λ0×0.58λ0. Then the antenna is developed to a two-element antenna array, working frequency and relative bandwidth of which are 2.15–2.87 GHz and 28.7%, respectively. Compared with antenna unit, the gain of the antenna array has increased by 2 dB. Thus the proposed antenna has characteristics of compact structure, relatively small size, and wideband, and it can be widely used in PCS/UMTS/WLAN/ WiMAX fields.

  13. Versatile, modular 3D microelectrode arrays for neuronal ensemble recordings: from design to fabrication, assembly, and functional validation in non-human primates

    Barz, F.; Livi, A.; Lanzilotto, M.; Maranesi, M.; Bonini, L.; Paul, O.; Ruther, P.

    2017-06-01

    Objective. Application-specific designs of electrode arrays offer an improved effectiveness for providing access to targeted brain regions in neuroscientific research and brain machine interfaces. The simultaneous and stable recording of neuronal ensembles is the main goal in the design of advanced neural interfaces. Here, we describe the development and assembly of highly customizable 3D microelectrode arrays and demonstrate their recording performance in chronic applications in non-human primates. Approach. System assembly relies on a microfabricated stacking component that is combined with Michigan-style silicon-based electrode arrays interfacing highly flexible polyimide cables. Based on the novel stacking component, the lead time for implementing prototypes with altered electrode pitches is minimal. Once the fabrication and assembly accuracy of the stacked probes have been characterized, their recording performance is assessed during in vivo chronic experiments in awake rhesus macaques (Macaca mulatta) trained to execute reaching-grasping motor tasks. Main results. Using a single set of fabrication tools, we implemented three variants of the stacking component for electrode distances of 250, 300 and 350 µm in the stacking direction. We assembled neural probes with up to 96 channels and an electrode density of 98 electrodes mm-2. Furthermore, we demonstrate that the shank alignment is accurate to a few µm at an angular alignment better than 1°. Three 64-channel probes were chronically implanted in two monkeys providing single-unit activity on more than 60% of all channels and excellent recording stability. Histological tissue sections, obtained 52 d after implantation from one of the monkeys, showed minimal tissue damage, in accordance with the high quality and stability of the recorded neural activity. Significance. The versatility of our fabrication and assembly approach should significantly support the development of ideal interface geometries for a broad

  14. Fluid Micro-Reservoirs Array Design with Auto-Pressure Regulation for High-Speed 3D Printers

    Moshe Einat

    2016-11-01

    Full Text Available Three dimensional (3D printing technology is rapidly evolving such that printing speed is now a crucial factor in technological developments and future applications. For printing heads based on the inkjet concept, the number of nozzles on the print head is a limiting factor of printing speed. This paper offers a method to practically increase the number of nozzles unlimitedly, and thus to dramatically ramp up printing speed. Fluid reservoirs are used in inkjet print heads to supply fluid through a manifold to the jetting chambers. The pressure in the reservoir’s outlet is important and influences device performance. Many efforts have been made to regulate pressure inside the fluid reservoirs so as to obtain a constant pressure in the chambers. When the number of nozzles is increased too much, the regulation of uniform pressure among all the nozzles becomes too complicated. In this paper, a different approach is taken. The reservoir is divided into an array of many micro-reservoirs. Each micro-reservoir supports one or a few chambers, and has a unique structure with auto-pressure regulation, where the outlet pressure is independent of the fluid level. The regulation is based on auto-compensation of the gravity force and a capillary force having the same dependence on the fluid level; this feature is obtained by adding a wedge in the reservoir with a unique shape. When the fluid level drops, the gravitational force and the capillary force decrease with it, but at similar rates. Terms for the force balance are derived and, consequently, a constant pressure in the fluid micro-reservoir segment is obtained automatically, with each segment being autonomous. This micro reservoir array is suggested for the enlargement of an inkjet print head and the achievement of high-speed 3D printing.

  15. Design of a 5-MA 100-ns linear-transformer-driver accelerator for wire array Z-pinch experiments

    Zhou, Lin; Li, Zhenghong; Wang, Zhen; Liang, Chuan; Li, Mingjia; Qi, Jianmin; Chu, Yanyun

    2016-03-01

    The linear-transformer-driver (LTD) is a recently developed pulsed-power technology that shows great promise for a number of applications. These include a Z -pinch-driven fission-fusion-hybrid reactor that is being developed by the Chinese Academy of Engineering Physics. In support of the reactor development effort, we are planning to build an LTD-based accelerator that is optimized for driving wire-array Z -pinch loads. The accelerator comprises six modules in parallel, each of which has eight series 0.8-MA LTD cavities in a voltage-adder configuration. Vacuum transmission lines are used from the interior of the adder to the central vacuum chamber where the load is placed. Thus the traditional stack-flashover problem is eliminated. The machine is 3.2 m tall and 12 m in outer diameter including supports. A prototype cavity was built and tested for more than 6000 shots intermittently at a repetition rate of 0.1 Hz. A novel trigger, in which only one input trigger pulse is needed by utilizing an internal trigger brick, was developed and successfully verified in these shots. A full circuit modeling was conducted for the accelerator. The simulation result shows that a current pulse rising to 5.2 MA in 91 ns (10%-90%) can be delivered to the wire-array load, which is 1.5 cm in height, 1.2 cm in initial radius, and 1 mg in mass. The maximum implosion velocity of the load is 32 cm /μ s when compressed to 0.1 of the initial radius. The maximum kinetic energy is 78 kJ, which is 11.7% of the electric energy stored in the capacitors. This accelerator is supposed to enable a radiation energy efficiency of 20%-30%, providing a high efficient facility for research on the fast Z pinch and technologies for repetition-rate-operated accelerators.

  16. SNP Arrays

    Jari Louhelainen

    2016-10-01

    Full Text Available The papers published in this Special Issue “SNP arrays” (Single Nucleotide Polymorphism Arrays focus on several perspectives associated with arrays of this type. The range of papers vary from a case report to reviews, thereby targeting wider audiences working in this field. The research focus of SNP arrays is often human cancers but this Issue expands that focus to include areas such as rare conditions, animal breeding and bioinformatics tools. Given the limited scope, the spectrum of papers is nothing short of remarkable and even from a technical point of view these papers will contribute to the field at a general level. Three of the papers published in this Special Issue focus on the use of various SNP array approaches in the analysis of three different cancer types. Two of the papers concentrate on two very different rare conditions, applying the SNP arrays slightly differently. Finally, two other papers evaluate the use of the SNP arrays in the context of genetic analysis of livestock. The findings reported in these papers help to close gaps in the current literature and also to give guidelines for future applications of SNP arrays.

  17. Fabrication of micro-channel arrays on thin metallic sheet using internal fluid pressure: Investigations on size effects and development of design guidelines

    Mahabunphachai, Sasawat [NSF I/UCR Center for Precision Forming, Department of Mechanical Engineering, Virginia Commonwealth University, Richmond, VA 23284 (United States); Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109 (United States); Koc, Muammer [NSF I/UCR Center for Precision Forming, Department of Mechanical Engineering, Virginia Commonwealth University, Richmond, VA 23284 (United States)

    2008-01-03

    Micro-feature (channel, protrusion, cavity, etc.) arrays on large area-thin metallic sheet alloys are increasingly needed for compact and integrated heat/mass transfer applications (such as fuel cells and fuel processors) that require high temperature resistance, corrosion resistance, good electrical/thermal conductivity, etc. The performance of these micro-feature arrays mainly affects the volume flow velocity of the reactants inside the arrays which directly controls the rate of convection mass/heat transport. The key factors that affect the flow velocity include channel size and shape, flow field pattern, flow path length, fluid pressure, etc. In this study, we investigated these micro-feature arrays from the manufacturability perspective since it is also an important factor to be considered in the design process. Internal fluid pressure (hydroforming) technique is investigated in this study with the specific goals to, first, understand if the so-called ''size effects'' (grain vs. feature size) are effective on the manufacturability of thin metallic sheet into micro-channels, and second, to establish design guidelines for the micro-channel hydroforming technique for robust mass production conditions. Thin stainless steel 304 blanks of 0.051 mm thick with three different grain sizes of 9.3, 10.6, and 17.0 {mu}m were used in hydroforming experiments to form micro-channels with the dimensions between 0.46-1.33 and 0.15-0.98 mm in width and height, respectively. Based on the experimental results, the effect of the grain size on the channel formability was found to be insignificant for the grain size range used in this study. On the other hand, the effect of the channel (feature) size was shown to dominate the overall formability. In addition, FE models of the process were developed and validated with the experimental results, then used to conduct a parametric study to establish micro-channel design guidelines. The results from the parametric

  18. Design concepts for the Cherenkov Telescope Array CTA: an advanced facility for ground-based high-energy gamma-ray astronomy

    Allekotte, I.; Arnaldi, H.; Asorey, H.; Gomez Berisso, M.; Sofo Haro, M.; Cillis, A.; Rovero, A.C.; Supanitsky, A.D.; Actis, M.; Antico, F.; Bottani, A.; Ochoa, I.; Ringegni, P.; Vallejo, G.; De La Vega, G.; Etchegoyen, A.; Videla, M.; Gonzalez, F.; Pallota, J.; Quel, E.; Ristori, P.; Romero, G.E.; Suarez, A.; Papyan, G.; Pogosyan, L.; Sahakian, V.; Bissaldi, E.; Egberts, K.; Reimer, A.; Reimer, O.; Shellard, R.C.; Santos, E.M.; De Gouveia Dal Pino, E.M.; Kowal, G.; De Souza, V.; Todero Peixoto, C.J.; Maneva, G.; Temnikov, P.; Vankov, H.; Golev, V.; Ovcharov, E.; Bonev, T.; Dimitrov, D.; Hrupec, D.; Nedbal, D.; Rob, L.; Sillanpaa, A.; Takalo, L.; Beckmann, V.; Benallou, M.; Boutonnet, C.; Corlier, M.; Courty, B.; Djannati-Atai, A.; Dufour, C.; Gabici, S.; Guglielmi, L.; Olivetto, C.; Pita, S.; Punch, M.; Selmane, S.; Terrier, R.; Yoffo, B.; Brun, P.; Carton, P.H.; Cazaux, S.; Corpace, O.; Delagnes, E.; Disset, G.; Durand, D.; Glicenstein, J.F.; Guilloux, F.; Kosack, K.; Medina, C.; Micolon, P.; Mirabel, F.; Moulin, E.; Peyaud, B.; Reymond, J.M.; Veyssiere, C.

    2011-01-01

    Ground-based gamma-ray astronomy has had a major breakthrough with the impressive results obtained using systems of imaging atmospheric Cherenkov telescopes. Ground-based gamma-ray astronomy has a huge potential in astrophysics, particle physics and cosmology. CTA is an international initiative to build the next generation instrument, with a factor of 5-10 improvement in sensitivity in the 100 GeV-10 TeV range and the extension to energies well below 100 GeV and above 100 TeV. CTA will consist of two arrays (one in the north, one in the south) for full sky coverage and will be operated as open observatory. The design of CTA is based on currently available technology. This document reports on the status and presents the major design concepts of CTA. (authors)

  19. electrode array

    PROF EKWUEME

    A geoelectric investigation employing vertical electrical soundings (VES) using the Ajayi - Makinde Two-Electrode array and the ... arrangements used in electrical D.C. resistivity survey. These include ..... Refraction Tomography to Study the.

  20. Design of a 5-MA 100-ns linear-transformer-driver accelerator for wire array Z-pinch experiments

    Zhou Lin

    2016-03-01

    Full Text Available The linear-transformer-driver (LTD is a recently developed pulsed-power technology that shows great promise for a number of applications. These include a Z-pinch-driven fission-fusion-hybrid reactor that is being developed by the Chinese Academy of Engineering Physics. In support of the reactor development effort, we are planning to build an LTD-based accelerator that is optimized for driving wire-array Z-pinch loads. The accelerator comprises six modules in parallel, each of which has eight series 0.8-MA LTD cavities in a voltage-adder configuration. Vacuum transmission lines are used from the interior of the adder to the central vacuum chamber where the load is placed. Thus the traditional stack-flashover problem is eliminated. The machine is 3.2 m tall and 12 m in outer diameter including supports. A prototype cavity was built and tested for more than 6000 shots intermittently at a repetition rate of 0.1 Hz. A novel trigger, in which only one input trigger pulse is needed by utilizing an internal trigger brick, was developed and successfully verified in these shots. A full circuit modeling was conducted for the accelerator. The simulation result shows that a current pulse rising to 5.2 MA in 91 ns (10%–90% can be delivered to the wire-array load, which is 1.5 cm in height, 1.2 cm in initial radius, and 1 mg in mass. The maximum implosion velocity of the load is 32  cm/μs when compressed to 0.1 of the initial radius. The maximum kinetic energy is 78 kJ, which is 11.7% of the electric energy stored in the capacitors. This accelerator is supposed to enable a radiation energy efficiency of 20%–30%, providing a high efficient facility for research on the fast Z pinch and technologies for repetition-rate-operated accelerators.

  1. Design of Arrangement of Solar Array and Energy Storage in Solar Photovoltaic Generation%光伏阵列及储能的布置设计

    韩学栋; 王海华

    2015-01-01

    Facing energy and environmental pressure, solar which is a kind natural renewable clean energy has become the focus of world development and utilization. This paper describes the optimal choice of the best dip angle of the solar array, deducing the layout spacing theoretical formula when the battery array in the horizontal plane and on the roof with a slope. Also, the paper describes the formula of the calculating components in series and parallel arrays of photovoltaic cells and storage cells in series and parallel. This paper provides some theoretical support to the design of the photovoltaic power generation system.%在能源和环境的双重压力下,太阳能这一清洁可再生的自然能源成为世界各国开发利用的重点。本文介绍了太阳能电池阵列最佳倾角的优化选择,推导了电池阵列在水平面以及带有一定坡度的屋面上进行布置时前后间距的理论计算公式,介绍了光伏电池阵列中组件的串并联以及储能蓄电池的串并联计算公式。上述工作对太阳能光伏发电系统的设计提供了一定的理论支撑。

  2. Design of Smart Ion-Selective Electrode Arrays Based on Source Separation through Nonlinear Independent Component Analysis

    Duarte L.T.

    2014-03-01

    Full Text Available The development of chemical sensor arrays based on Blind Source Separation (BSS provides a promising solution to overcome the interference problem associated with Ion-Selective Electrodes (ISE. The main motivation behind this new approach is to ease the time-demanding calibration stage. While the first works on this problem only considered the case in which the ions under analysis have equal valences, the present work aims at developing a BSS technique that works when the ions have different charges. In this situation, the resulting mixing model belongs to a particular class of nonlinear systems that have never been studied in the BSS literature. In order to tackle this sort of mixing process, we adopted a recurrent network as separating system. Moreover, concerning the BSS learning strategy, we develop a mutual information minimization approach based on the notion of the differential of the mutual information. The method works requires a batch operation, and, thus, can be used to perform off-line analysis. The validity of our approach is supported by experiments where the mixing model parameters were extracted from actual data.

  3. Design and reliability analysis of high-speed and continuous data recording system based on disk array

    Jiang, Changlong; Ma, Cheng; He, Ning; Zhang, Xugang; Wang, Chongyang; Jia, Huibo

    2002-12-01

    In many real-time fields the sustained high-speed data recording system is required. This paper proposes a high-speed and sustained data recording system based on the complex-RAID 3+0. The system consists of Array Controller Module (ACM), String Controller Module (SCM) and Main Controller Module (MCM). ACM implemented by an FPGA chip is used to split the high-speed incoming data stream into several lower-speed streams and generate one parity code stream synchronously. It also can inversely recover the original data stream while reading. SCMs record lower-speed streams from the ACM into the SCSI disk drivers. In the SCM, the dual-page buffer technology is adopted to implement speed-matching function and satisfy the need of sustainable recording. MCM monitors the whole system, controls ACM and SCMs to realize the data stripping, reconstruction, and recovery functions. The method of how to determine the system scale is presented. At the end, two new ways Floating Parity Group (FPG) and full 2D-Parity Group (full 2D-PG) are proposed to improve the system reliability and compared with the Traditional Parity Group (TPG). This recording system can be used conveniently in many areas of data recording, storing, playback and remote backup with its high-reliability.

  4. Identification of novel candidate target genes in amplicons of Glioblastoma multiforme tumors detected by expression and CGH microarray profiling

    Hernández-Moneo Jose-Luis

    2006-09-01

    Full Text Available Abstract Background Conventional cytogenetic and comparative genomic hybridization (CGH studies in brain malignancies have shown that glioblastoma multiforme (GBM is characterized by complex structural and numerical alterations. However, the limited resolution of these techniques has precluded the precise identification of detailed specific gene copy number alterations. Results We performed a genome-wide survey of gene copy number changes in 20 primary GBMs by CGH on cDNA microarrays. A novel amplicon at 4p15, and previously uncharacterized amplicons at 13q32-34 and 1q32 were detected and are analyzed here. These amplicons contained amplified genes not previously reported. Other amplified regions containg well-known oncogenes in GBMs were also detected at 7p12 (EGFR, 7q21 (CDK6, 4q12 (PDGFRA, and 12q13-15 (MDM2 and CDK4. In order to identify the putative target genes of the amplifications, and to determine the changes in gene expression levels associated with copy number change events, we carried out parallel gene expression profiling analyses using the same cDNA microarrays. We detected overexpression of the novel amplified genes SLA/LP and STIM2 (4p15, and TNFSF13B and COL4A2 (13q32-34. Some of the candidate target genes of amplification (EGFR, CDK6, MDM2, CDK4, and TNFSF13B were tested in an independent set of 111 primary GBMs by using FISH and immunohistological assays. The novel candidate 13q-amplification target TNFSF13B was amplified in 8% of the tumors, and showed protein expression in 20% of the GBMs. Conclusion This high-resolution analysis allowed us to propose novel candidate target genes such as STIM2 at 4p15, and TNFSF13B or COL4A2 at 13q32-34 that could potentially contribute to the pathogenesis of these tumors and which would require futher investigations. We showed that overexpression of the amplified genes could be attributable to gene dosage and speculate that deregulation of those genes could be important in the development

  5. Combined M-FISH and CGH analysis allows comprehensive description of genetic alterations in neuroblastoma cell lines.

    Van Roy, N; Van Limbergen, H; Vandesompele, J; Van Gele, M; Poppe, B; Salwen, H; Laureys, G; Manoel, N; De Paepe, A; Speleman, F

    2001-10-01

    Cancer cell lines are essential gene discovery tools and have often served as models in genetic and functional studies of particular tumor types. One of the future challenges is comparison and interpretation of gene expression data with the available knowledge on the genomic abnormalities in these cell lines. In this context, accurate description of these genomic abnormalities is required. Here, we show that a combination of M-FISH with banding analysis, standard FISH, and CGH allowed a detailed description of the genetic alterations in 16 neuroblastoma cell lines. In total, 14 cryptic chromosome rearrangements were detected, including a balanced t(2;4)(p24.3;q34.3) translocation in cell line NBL-S, with the 2p24 breakpoint located at about 40 kb from MYCN. The chromosomal origin of 22 marker chromosomes and 41 cytogenetically undefined translocated segments was determined. Chromosome arm 2 short arm translocations were observed in six cell lines (38%) with and five (31%) without MYCN amplification, leading to partial chromosome arm 2p gain in all but one cell line and loss of material in the various partner chromosomes, including 1p and 11q. These 2p gains were often masked in the GGH profiles due to MYCN amplification. The commonly overrepresented region was chromosome segment 2pter-2p22, which contains the MYCN gene, and five out of eleven 2p breakpoints clustered to the interface of chromosome bands 2p16 and 2p21. In neuroblastoma cell line SJNB-12, with double minutes (dmins) but no MYCN amplification, the dmins were shown to be derived from 16q22-q23 sequences. The ATBF1 gene, an AT-binding transcription factor involved in normal neurogenesis and located at 16q22.2, was shown to be present in the amplicon. This is the first report describing the possible implication of ATBF1 in neuroblastoma cells. We conclude that a combined approach of M-FISH, cytogenetics, and CGH allowed a more complete and accurate description of the genetic alterations occurring in the

  6. Microneedle Array Design Determines the Induction of Protective Memory CD8+ T Cell Responses Induced by a Recombinant Live Malaria Vaccine in Mice

    Carey, John B.; Pearson, Frances E.; Vrdoljak, Anto; McGrath, Marie G.; Crean, Abina M.; Walsh, Patrick T.; Doody, Timothy; O'Mahony, Conor; Hill, Adrian V. S.; Moore, Anne C.

    2011-01-01

    Background Vaccine delivery into the skin has received renewed interest due to ease of access to the immune system and microvasculature, however the stratum corneum (SC), must be breached for successful vaccination. This has been achieved by removing the SC by abrasion or scarification or by delivering the vaccine intradermally (ID) with traditional needle-and-syringes or with long microneedle devices. Microneedle patch-based transdermal vaccine studies have predominantly focused on antibody induction by inactivated or subunit vaccines. Here, our principal aim is to determine if the design of a microneedle patch affects the CD8+ T cell responses to a malaria antigen induced by a live vaccine. Methodology and Findings Recombinant modified vaccinia virus Ankara (MVA) expressing a malaria antigen was percutaneously administered to mice using a range of silicon microneedle patches, termed ImmuPatch, that differed in microneedle height, density, patch area and total pore volume. We demonstrate that microneedle arrays that have small total pore volumes induce a significantly greater proportion of central memory T cells that vigorously expand to secondary immunization. Microneedle-mediated vaccine priming induced significantly greater T cell immunity post-boost and equivalent protection against malaria challenge compared to ID vaccination. Notably, unlike ID administration, ImmuPatch-mediated vaccination did not induce inflammatory responses at the site of immunization or in draining lymph nodes. Conclusions/Significance This study demonstrates that the design of microneedle patches significantly influences the magnitude and memory of vaccine-induced CD8+ T cell responses and can be optimised for the induction of desired immune responses. Furthermore, ImmuPatch-mediated delivery may be of benefit to reducing unwanted vaccine reactogenicity. In addition to the advantages of low cost and lack of pain, the development of optimised microneedle array designs for the induction

  7. Microneedle array design determines the induction of protective memory CD8+ T cell responses induced by a recombinant live malaria vaccine in mice.

    John B Carey

    Full Text Available Vaccine delivery into the skin has received renewed interest due to ease of access to the immune system and microvasculature, however the stratum corneum (SC, must be breached for successful vaccination. This has been achieved by removing the SC by abrasion or scarification or by delivering the vaccine intradermally (ID with traditional needle-and-syringes or with long microneedle devices. Microneedle patch-based transdermal vaccine studies have predominantly focused on antibody induction by inactivated or subunit vaccines. Here, our principal aim is to determine if the design of a microneedle patch affects the CD8(+ T cell responses to a malaria antigen induced by a live vaccine.Recombinant modified vaccinia virus Ankara (MVA expressing a malaria antigen was percutaneously administered to mice using a range of silicon microneedle patches, termed ImmuPatch, that differed in microneedle height, density, patch area and total pore volume. We demonstrate that microneedle arrays that have small total pore volumes induce a significantly greater proportion of central memory T cells that vigorously expand to secondary immunization. Microneedle-mediated vaccine priming induced significantly greater T cell immunity post-boost and equivalent protection against malaria challenge compared to ID vaccination. Notably, unlike ID administration, ImmuPatch-mediated vaccination did not induce inflammatory responses at the site of immunization or in draining lymph nodes.This study demonstrates that the design of microneedle patches significantly influences the magnitude and memory of vaccine-induced CD8(+ T cell responses and can be optimised for the induction of desired immune responses. Furthermore, ImmuPatch-mediated delivery may be of benefit to reducing unwanted vaccine reactogenicity. In addition to the advantages of low cost and lack of pain, the development of optimised microneedle array designs for the induction of T cell responses by live vaccines aids

  8. A Nonlinear Model for Designing Herschel-Quincke Waveguide Arrays to Attenuate Shock Waves from Transonic Turbofan Engines, Phase I

    National Aeronautics and Space Administration — Techsburg is teaming with the Vibration and Acoustics Laboratory of Virginia Tech to propose a non-linear analytical tool for designing Herschel-Quincke (HQ)...

  9. SKALA, a log-periodic array antenna for the SKA-low instrument: design, simulations, tests and system considerations

    de Lera Acedo, E.; Razavi-Ghods, N.; Troop, N.; Drought, N.; Faulkner, A. J.

    2015-10-01

    The very demanding requirements of the SKA-low instrument call for a challenging antenna design capable of delivering excellent performance in radiation patterns, impedance matching, polarization purity, cost, longevity, etc. This paper is devoted to the development (design and test of the first prototypes) of an active ultra-wideband antenna element for the low-frequency instrument of the SKA radio telescope. The antenna element and differential low noise amplifier described here were originally designed to cover the former SKA-low band (70-450 MHz) but it is now aimed to cover the re-defined SKA-low band (50-350 MHz) and furthermore the antenna is capable of performing up to 650 MHz with the current design. The design is focused on maximum sensitivity in a wide field of view (+/- 45° from zenith) and low cross-polarization ratios. Furthermore, the size and cost of the element has to be kept to a minimum as millions of these antennas will need to be deployed for the full SKA in very compact configurations. The primary focus of this paper is therefore to discuss various design implications for the SKA-low telescope.

  10. Genetic Algorithm Design And Testing of a Random Element 3-D 2.4 Ghz Phased Array Transmit Antenna Constructed of Commercial Rf Microchips

    Esswein, Lance

    2003-01-01

    ..., development and evaluation of a test-bed array. The test-bed array was constructed of commercially available components, including a unique and innovative application of a quadrature modulator microchip used in commercial communications applications...

  11. Design of a high-gain laser diode-array pumped Nd:YAG alternating precessive slab amplifier (APS amplifier)

    Coyle, D. B.

    1991-01-01

    In the design of space-qualifiable laser systems for ranging and altimetry, such as NASA's Geodynamic Laser Ranging System (GLRS), the transmitter must be kept small, powerful yet efficient, and must consist of as few components as possible. A novel preamplifier design is examined which requires no external beam steering optics, yielding a compact component with simple alignment procedures. The gains achieved are comparable to multipass zigzag amplifiers using two or more sets of external optics for extra passes through the amplifying medium.

  12. Final report on the scientific and engineering design of a soft X-ray diode array diagnostic system for JET (KJ 1)

    Fahrbach, H.U.; Goss, H.; Harmeyer, E.; Schramm, G.

    1982-07-01

    This report describes the Soft-X-Ray Diode Array Diagnostic System for JET. It was designed by the IPP under an Article 14 contract for Phase I. The diagnostics will be capable of measuring the Soft-X-ray emission from H and D plasmas in JET with high resolution in space and time. Two slot-hole cameras with 150 detectors viewing the same toroidal cross-section of the plasma are foreseen. Thin beryllium and aluminium filters in the line of sight of the detectors allow simultaneous measurements within different limits of the X-ray spectrum. Heavy shielding against neutron and gamma radiation is provided in order to reduce radiation induced signals and to increase detector lifetimes. The signals of 100 detectors can be simultaneously sampled with a sampling rate variable up to 250 KHz and stored in 12 bit memories of about 20 Kwords size. (orig.)

  13. Filter arrays

    Page, Ralph H.; Doty, Patrick F.

    2017-08-01

    The various technologies presented herein relate to a tiled filter array that can be used in connection with performance of spatial sampling of optical signals. The filter array comprises filter tiles, wherein a first plurality of filter tiles are formed from a first material, the first material being configured such that only photons having wavelengths in a first wavelength band pass therethrough. A second plurality of filter tiles is formed from a second material, the second material being configured such that only photons having wavelengths in a second wavelength band pass therethrough. The first plurality of filter tiles and the second plurality of filter tiles can be interspersed to form the filter array comprising an alternating arrangement of first filter tiles and second filter tiles.

  14. Cascading Constrained 2-D Arrays using Periodic Merging Arrays

    Forchhammer, Søren; Laursen, Torben Vaarby

    2003-01-01

    We consider a method for designing 2-D constrained codes by cascading finite width arrays using predefined finite width periodic merging arrays. This provides a constructive lower bound on the capacity of the 2-D constrained code. Examples include symmetric RLL and density constrained codes...

  15. Design verification enhancement of field programmable gate array-based safety-critical I&C system of nuclear power plant

    Ahmed, Ibrahim [Department of Nuclear Engineering, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104 (Korea, Republic of); Jung, Jaecheon, E-mail: jcjung@kings.ac.kr [Department of Nuclear Power Plant Engineering, KEPCO International Nuclear Graduate School, 658-91 Haemaji-ro, Seosang-myeon, Ulju-gun, Ulsan 45014 (Korea, Republic of); Heo, Gyunyoung [Department of Nuclear Engineering, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104 (Korea, Republic of)

    2017-06-15

    Highlights: • An enhanced, systematic and integrated design verification approach is proposed for V&V of FPGA-based I&C system of NPP. • RPS bistable fixed setpoint trip algorithm is designed, analyzed, verified and discussed using the proposed approaches. • The application of integrated verification approach simultaneously verified the entire design modules. • The applicability of the proposed V&V facilitated the design verification processes. - Abstract: Safety-critical instrumentation and control (I&C) system in nuclear power plant (NPP) implemented on programmable logic controllers (PLCs) plays a vital role in safe operation of the plant. The challenges such as fast obsolescence, the vulnerability to cyber-attack, and other related issues of software systems have currently led to the consideration of field programmable gate arrays (FPGAs) as an alternative to PLCs because of their advantages and hardware related benefits. However, safety analysis for FPGA-based I&C systems, and verification and validation (V&V) assessments still remain important issues to be resolved, which are now become a global research point of interests. In this work, we proposed a systematic design and verification strategies from start to ready-to-use in form of model-based approaches for FPGA-based reactor protection system (RPS) that can lead to the enhancement of the design verification and validation processes. The proposed methodology stages are requirement analysis, enhanced functional flow block diagram (EFFBD) models, finite state machine with data path (FSMD) models, hardware description language (HDL) code development, and design verifications. The design verification stage includes unit test – Very high speed integrated circuit Hardware Description Language (VHDL) test and modified condition decision coverage (MC/DC) test, module test – MATLAB/Simulink Co-simulation test, and integration test – FPGA hardware test beds. To prove the adequacy of the proposed

  16. Design verification enhancement of field programmable gate array-based safety-critical I&C system of nuclear power plant

    Ahmed, Ibrahim; Jung, Jaecheon; Heo, Gyunyoung

    2017-01-01

    Highlights: • An enhanced, systematic and integrated design verification approach is proposed for V&V of FPGA-based I&C system of NPP. • RPS bistable fixed setpoint trip algorithm is designed, analyzed, verified and discussed using the proposed approaches. • The application of integrated verification approach simultaneously verified the entire design modules. • The applicability of the proposed V&V facilitated the design verification processes. - Abstract: Safety-critical instrumentation and control (I&C) system in nuclear power plant (NPP) implemented on programmable logic controllers (PLCs) plays a vital role in safe operation of the plant. The challenges such as fast obsolescence, the vulnerability to cyber-attack, and other related issues of software systems have currently led to the consideration of field programmable gate arrays (FPGAs) as an alternative to PLCs because of their advantages and hardware related benefits. However, safety analysis for FPGA-based I&C systems, and verification and validation (V&V) assessments still remain important issues to be resolved, which are now become a global research point of interests. In this work, we proposed a systematic design and verification strategies from start to ready-to-use in form of model-based approaches for FPGA-based reactor protection system (RPS) that can lead to the enhancement of the design verification and validation processes. The proposed methodology stages are requirement analysis, enhanced functional flow block diagram (EFFBD) models, finite state machine with data path (FSMD) models, hardware description language (HDL) code development, and design verifications. The design verification stage includes unit test – Very high speed integrated circuit Hardware Description Language (VHDL) test and modified condition decision coverage (MC/DC) test, module test – MATLAB/Simulink Co-simulation test, and integration test – FPGA hardware test beds. To prove the adequacy of the proposed

  17. Microbial Diagnostic Array Workstation (MDAW: a web server for diagnostic array data storage, sharing and analysis

    Chang Yung-Fu

    2008-09-01

    Full Text Available Abstract Background Microarrays are becoming a very popular tool for microbial detection and diagnostics. Although these diagnostic arrays are much simpler when compared to the traditional transcriptome arrays, due to the high throughput nature of the arrays, the data analysis requirements still form a bottle neck for the widespread use of these diagnostic arrays. Hence we developed a new online data sharing and analysis environment customised for diagnostic arrays. Methods Microbial Diagnostic Array Workstation (MDAW is a database driven application designed in MS Access and front end designed in ASP.NET. Conclusion MDAW is a new resource that is customised for the data analysis requirements for microbial diagnostic arrays.

  18. Design and construction of hierarchical TiO2 nanorod arrays by combining layer-by-layer and hydrothermal crystallization techniques for electrochromic application

    Chen, Yongbo; Li, Xiaomin; Bi, Zhijie; He, Xiaoli; Li, Guanjie; Xu, Xiaoke; Gao, Xiangdong

    2018-05-01

    The hierarchical TiO2 (H-TiO2) nanorod arrays (NRAs) composed of single-crystalline nanorods and nanocrystals were finely designed and successfully constructed for electrochromic (EC) application. By combining layer-by-layer (LBL) method and hydrothermal crystallization technique, the superfine nanocrystals (5-7 nm), which can provide abundant active sites and facilitate ion insertion/extraction during EC reactions, were uniformly and conformally assembled on the surface of single-crystalline TiO2 (SC-TiO2) NRAs. The as-formed H-TiO2 NRAs integrate the advantages of one-dimensional NRAs with fast kinetics and superfine nanocrystals with high ion capacity, showing highly enhanced EC performance. Large optical contrast (40.3%), shorter coloring/bleaching time (22/4 s), high coloration efficiency (11.2 cm2 C-1), and excellent cycling stability can be achieved in H-TiO2 NRAs, superior to the pristine SC-TiO2 NRAs and nanocrystalline TiO2 films. This work provides a feasible and well-designed strategy to explore high-performance materials for EC application.

  19. Detection and precise mapping of germline rearrangements in BRCA1, BRCA2, MSH2, and MLH1 using zoom-in array comparative genomic hybridization (aCGH)

    Staaf, Johan; Törngren, Therese; Rambech, Eva

    2008-01-01

    deletions or duplications occurring in BRCA1 (n=11), BRCA2 (n=2), MSH2 (n=7), or MLH1 (n=9). Additionally, we demonstrate its applicability for uncovering complex somatic rearrangements, exemplified by zoom-in analysis of the PTEN and CDKN2A loci in breast cancer cells. The sizes of rearrangements ranged...

  20. Tomographic array

    1976-01-01

    The configuration of a tomographic array in which the object can rotate about its axis is described. The X-ray detector is a cylindrical screen perpendicular to the axis of rotation. The X-ray source has a line-shaped focus coinciding with the axis of rotation. The beam is fan-shaped with one side of this fan lying along the axis of rotation. The detector screen is placed inside an X-ray image multiplier tube

  1. Tomographic array

    1976-01-01

    A tomographic array with the following characteristics is described. An X-ray screen serving as detector is placed before a photomultiplier tube which itself is placed in front of a television camera connected to a set of image processors. The detector is concave towards the source and is replacable. Different images of the object are obtained simultaneously. Optical fibers and lenses are used for transmission within the system

  2. Preimplantation genetic diagnosis for chromosomal rearrangements with the use of array comparative genomic hybridization at the blastocyst stage.

    Christodoulou, Christodoulos; Dheedene, Annelies; Heindryckx, Björn; van Nieuwerburgh, Filip; Deforce, Dieter; De Sutter, Petra; Menten, Björn; Van den Abbeel, Etienne

    2017-01-01

    To establish the value of array comparative genomic hybridization (CGH) for preimplantation genetic diagnosis (PGD) in embryos of translocation carriers in combination with vitrification and frozen embryo transfer in nonstimulated cycles. Retrospective data analysis study. Academic centers for reproductive medicine and genetics. Thirty-four couples undergoing PGD for chromosomal rearrangements from October 2013 to December 2015. Trophectoderm biopsy at day 5 or day 6 of embryo development and subsequently whole genome amplification and array CGH were performed. This approach revealed a high occurrence of aneuploidies and structural rearrangements unrelated to the parental rearrangement. Nevertheless, we observed a benefit in pregnancy rates of these couples. We detected chromosomal abnormalities in 133/207 embryos (64.2% of successfully amplified), and 74 showed a normal microarray profile (35.7%). In 48 of the 133 abnormal embryos (36.1%), an unbalanced rearrangement originating from the parental translocation was identified. Interestingly, 34.6% of the abnormal embryos (46/133) harbored chromosome rearrangements that were not directly linked to the parental translocation in question. We also detected a combination of unbalanced parental-derived rearrangements and aneuploidies in 27 of the 133 abnormal embryos (20.3%). The use of trophectoderm biopsy at the blastocyst stage is less detrimental to the survival of the embryo and leads to a more reliable estimate of the genomic content of the embryo than cleavage-stage biopsy. In this small cohort PGD study, we describe the successful implementation of array CGH analysis of blastocysts in patients with a chromosomal rearrangement to identify euploid embryos for transfer. Copyright © 2016 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

  3. The Big Optical Array

    Mozurkewich, D.; Johnston, K.J.; Simon, R.S.

    1990-01-01

    This paper describes the design and the capabilities of the Naval Research Laboratory Big Optical Array (BOA), an interferometric optical array for high-resolution imaging of stars, stellar systems, and other celestial objects. There are four important differences between the BOA design and the design of Mark III Optical Interferometer on Mount Wilson (California). These include a long passive delay line which will be used in BOA to do most of the delay compensation, so that the fast delay line will have a very short travel; the beam combination in BOA will be done in triplets, to allow measurement of closure phase; the same light will be used for both star and fringe tracking; and the fringe tracker will use several wavelength channels

  4. Design and operation of a high-heat flux, flush-mounted ‘rail’ Langmuir probe array on Alcator C-Mod

    A.Q. Kuang

    2017-08-01

    Full Text Available A poloidal array of toroidally-extended, flush-mounted ‘rail’ Langmuir probes was recently installed on Alcator C-Mod's vertical target plate divertor. The aim was to investigate if a Langmuir probe array could be designed to survive reactor-level heat fluxes and have the ability to make measurements that could be reliably interpreted under reactor-level plasma densities, neutral densities and magnetic fields. Langmuir probes are typically built to have incident field-line angles >10° to avoid interpretation issues associated with sheath expansion. However, at the high parallel heat fluxes experienced in reactor-relevant conditions such a probe would quickly overheat and melt. To mitigate both the issues of extreme heat flux and sheath expansion, each probe was designed to be flush with the divertor surface, toroidally-extended and field-aligned, giving it a ‘rail’ geometry. The flush mounted probes have proven to be exceptionally robust surviving the 2015–2016 campaign – a first for a C-Mod probe system. Examination of the probe current-voltage (I-V characteristics reveals that they are immune to sheath expansion at incident field angles down to ∼0.5°. Comparison of the flush probes to traditional proud probes shows that both measure the same electron pressure across the divertor plate. However, there are significant and systematic differences in the density, temperature and floating potential. This suggests that there is important physics, perhaps unique to conditions in a vertical-target plate divertor with small field-line attack angles, that affects the I-V characteristics and is not currently included in probe data analyses. Finally, the probe response is examined in the ‘death-ray’ regime, just near detachment. Previous work using proud probes has suggested that the ‘death-ray’ is an artefact of the probe bias. However, on flush mounted probes the ‘death-ray’ manifests itself under different conditions, which

  5. Maximum gain of Yagi-Uda arrays

    Bojsen, J.H.; Schjær-Jacobsen, Hans; Nilsson, E.

    1971-01-01

    Numerical optimisation techniques have been used to find the maximum gain of some specific parasitic arrays. The gain of an array of infinitely thin, equispaced dipoles loaded with arbitrary reactances has been optimised. The results show that standard travelling-wave design methods are not optimum....... Yagi–Uda arrays with equal and unequal spacing have also been optimised with experimental verification....

  6. Microneedle array electrode for human EEG recording.

    Lüttge, Regina; van Nieuwkasteele-Bystrova, Svetlana Nikolajevna; van Putten, Michel Johannes Antonius Maria; Vander Sloten, Jos; Verdonck, Pascal; Nyssen, Marc; Haueisen, Jens

    2009-01-01

    Microneedle array electrodes for EEG significantly reduce the mounting time, particularly by circumvention of the need for skin preparation by scrubbing. We designed a new replication process for numerous types of microneedle arrays. Here, polymer microneedle array electrodes with 64 microneedles,

  7. Design of a phased array for the generation of adaptive radiation force along a path surrounding a breast lesion for dynamic ultrasound elastography imaging.

    Ekeom, Didace; Hadj Henni, Anis; Cloutier, Guy

    2013-03-01

    This work demonstrates, with numerical simulations, the potential of an octagonal probe for the generation of radiation forces in a set of points following a path surrounding a breast lesion in the context of dynamic ultrasound elastography imaging. Because of the in-going wave adaptive focusing strategy, the proposed method is adapted to induce shear wave fronts to interact optimally with complex lesions. Transducer elements were based on 1-3 piezocomposite material. Three-dimensional simulations combining the finite element method and boundary element method with periodic boundary conditions in the elevation direction were used to predict acoustic wave radiation in a targeted region of interest. The coupling factor of the piezocomposite material and the radiated power of the transducer were optimized. The transducer's electrical impedance was targeted to 50 Ω. The probe was simulated by assembling the designed transducer elements to build an octagonal phased-array with 256 elements on each edge (for a total of 2048 elements). The central frequency is 4.54 MHz; simulated transducer elements are able to deliver enough power and can generate the radiation force with a relatively low level of voltage excitation. Using dynamic transmitter beamforming techniques, the radiation force along a path and resulting acoustic pattern in the breast were simulated assuming a linear isotropic medium. Magnitude and orientation of the acoustic intensity (radiation force) at any point of a generation path could be controlled for the case of an example representing a heterogeneous medium with an embedded soft mechanical inclusion.

  8. Large scale biomimetic membrane arrays

    Hansen, Jesper Søndergaard; Perry, Mark; Vogel, Jörg

    2009-01-01

    To establish planar biomimetic membranes across large scale partition aperture arrays, we created a disposable single-use horizontal chamber design that supports combined optical-electrical measurements. Functional lipid bilayers could easily and efficiently be established across CO2 laser micro......-structured 8 x 8 aperture partition arrays with average aperture diameters of 301 +/- 5 mu m. We addressed the electro-physical properties of the lipid bilayers established across the micro-structured scaffold arrays by controllable reconstitution of biotechnological and physiological relevant membrane...... peptides and proteins. Next, we tested the scalability of the biomimetic membrane design by establishing lipid bilayers in rectangular 24 x 24 and hexagonal 24 x 27 aperture arrays, respectively. The results presented show that the design is suitable for further developments of sensitive biosensor assays...

  9. Digital electrostatic acoustic transducer array

    Carreno, Armando Arpys Arevalo

    2016-12-19

    In this paper we present the fabrication and characterization of an array of electrostatic acoustic transducers. The array is micromachined on a silicon wafer using standard micro-machining techniques. Each array contains 2n electrostatic transducer membranes, where “n” is the bit number. Every element of the array has a hexagonal membrane shape structure, which is separated from the substrate by 3µm air gap. The membrane is made out 5µm thick polyimide layer that has a bottom gold electrode on the substrate and a gold top electrode on top of the membrane (250nm). The wafer layout design was diced in nine chips with different array configurations, with variation of the membrane dimensions. The device was tested with 90 V giving and sound output level as high as 35dB, while actuating all the elements at the same time.

  10. Digital electrostatic acoustic transducer array

    Carreno, Armando Arpys Arevalo; Castro, David; Conchouso Gonzalez, David; Kosel, Jü rgen; Foulds, Ian G.

    2016-01-01

    In this paper we present the fabrication and characterization of an array of electrostatic acoustic transducers. The array is micromachined on a silicon wafer using standard micro-machining techniques. Each array contains 2n electrostatic transducer membranes, where “n” is the bit number. Every element of the array has a hexagonal membrane shape structure, which is separated from the substrate by 3µm air gap. The membrane is made out 5µm thick polyimide layer that has a bottom gold electrode on the substrate and a gold top electrode on top of the membrane (250nm). The wafer layout design was diced in nine chips with different array configurations, with variation of the membrane dimensions. The device was tested with 90 V giving and sound output level as high as 35dB, while actuating all the elements at the same time.

  11. Processes for design, construction and utilisation of arrays of light-emitting diodes and light-emitting diode-coupled optical fibres for multi-site brain light delivery.

    Bernstein, Jacob G; Allen, Brian D; Guerra, Alexander A; Boyden, Edward S

    2015-05-01

    Optogenetics enables light to be used to control the activity of genetically targeted cells in the living brain. Optical fibers can be used to deliver light to deep targets, and LEDs can be spatially arranged to enable patterned light delivery. In combination, arrays of LED-coupled optical fibers can enable patterned light delivery to deep targets in the brain. Here we describe the process flow for making LED arrays and LED-coupled optical fiber arrays, explaining key optical, electrical, thermal, and mechanical design principles to enable the manufacturing, assembly, and testing of such multi-site targetable optical devices. We also explore accessory strategies such as surgical automation approaches as well as innovations to enable low-noise concurrent electrophysiology.

  12. Prognostic Impact of Array-based Genomic Profiles in Esophageal Squamous Cell Cancer

    Carneiro, Ana; Isinger, Anna; Karlsson, Anna; Johansson, Jan; Jönsson, Göran; Bendahl, Pär-Ola; Falkenback, Dan; Halvarsson, Britta; Nilbert, Mef

    2008-01-01

    Esophageal squamous cell carcinoma (ESCC) is a genetically complex tumor type and a major cause of cancer related mortality. Although distinct genetic alterations have been linked to ESCC development and prognosis, the genetic alterations have not gained clinical applicability. We applied array-based comparative genomic hybridization (aCGH) to obtain a whole genome copy number profile relevant for identifying deranged pathways and clinically applicable markers. A 32 k aCGH platform was used for high resolution mapping of copy number changes in 30 stage I-IV ESCC. Potential interdependent alterations and deranged pathways were identified and copy number changes were correlated to stage, differentiation and survival. Copy number alterations affected median 19% of the genome and included recurrent gains of chromosome regions 5p, 7p, 7q, 8q, 10q, 11q, 12p, 14q, 16p, 17p, 19p, 19q, and 20q and losses of 3p, 5q, 8p, 9p and 11q. High-level amplifications were observed in 30 regions and recurrently involved 7p11 (EGFR), 11q13 (MYEOV, CCND1, FGF4, FGF3, PPFIA, FAD, TMEM16A, CTTS and SHANK2) and 11q22 (PDFG). Gain of 7p22.3 predicted nodal metastases and gains of 1p36.32 and 19p13.3 independently predicted poor survival in multivariate analysis. aCGH profiling verified genetic complexity in ESCC and herein identified imbalances of multiple central tumorigenic pathways. Distinct gains correlate with clinicopathological variables and independently predict survival, suggesting clinical applicability of genomic profiling in ESCC

  13. Fundamentals of ultrasonic phased arrays

    Schmerr, Lester W

    2014-01-01

    This book describes in detail the physical and mathematical foundations of ultrasonic phased array measurements.?The book uses linear systems theory to develop a comprehensive model of the signals and images that can be formed with phased arrays. Engineers working in the field of ultrasonic nondestructive evaluation (NDE) will find in this approach a wealth of information on how to design, optimize and interpret ultrasonic inspections with phased arrays. The fundamentals and models described in the book will also be of significant interest to other fields, including the medical ultrasound and

  14. Photovoltaic array: Power conditioner interface characteristics

    Gonzalez, C. C.; Hill, G. M.; Ross, R. G., Jr.

    1982-01-01

    The electrical output (power, current, and voltage) of flat plate solar arrays changes constantly, due primarily to changes in cell temperature and irradiance level. As a result, array loads such as dc-to-ac power conditioners must be capable of accommodating widely varying input levels while maintaining operation at or near the maximum power point of the array. The array operating characteristics and extreme output limits necessary for the systematic design of array load interfaces under a wide variety of climatic conditions are studied. A number of interface parameters are examined, including optimum operating voltage, voltage energy, maximum power and current limits, and maximum open circuit voltage. The effect of array degradation and I-V curve fill factor or the array power conditioner interface is also discussed. Results are presented as normalized ratios of power conditioner parameters to array parameters, making the results universally applicable to a wide variety of system sizes, sites, and operating modes.

  15. Halbach arrays in precision motion control

    Trumper, D.L.; Williams, M.E. [Massachusetts Institute of Technology, Cambridge, MA (United States)

    1995-02-01

    The Halbach array was developed for use as an optical element in particle accelerators. Following up on a suggestion from Klaus Halbach, the authors have investigated the utility of such arrays as the permanent magnet structure for synchronous machines in cartesian, polar, and cylindrical geometries. Their work has focused on the design of a novel Halbach array linear motor for use in a magnetic suspension stage for photolithography. This paper presents the details of the motor design and its force and power characteristics.

  16. Enhancement of anti-tubercular activity and biomass of fermented food associated Staphylococcus hominis strain MANF2 using Taguchi orthogonal array and Box-Behnken design.

    Khusro, Ameer; Aarti, Chirom; Dusthackeer, Azger; Agastian, Paul

    2018-04-14

    The prime focus of the present investigation was to optimize statistically the anti-tubercular activity and biomass of fermented food associated Staphylococcus hominis strain MANF2 using Taguchi orthogonal array (OA) and Box-Behnken design (BBD). The anti-tubercular activity of strain MANF2 was determined against Mycobacterium tuberculosis H37Rv using luciferase reporter phase assay. Among varied media examined, the isolate exhibited impressive anti-tubercular activity with paramount relative light unit reduction of >90% in de Man Rogose Sharpe (MRS) broth. Primarily, the anti-tubercular activity and biomass of strain MANF2 were estimated in MRS broth by optimizing eight diversified parameters using one factor at a time (OFAT) method after working out a series of experiments. The most significant contributing factors selected through OFAT tool were optimized using Taguchi approach with a standard OA layout of L18 (2 2  × 3 6 ). Results demonstrated the significant (P ≤ 0.05) influence of pH, temperature, yeast extract, magnesium sulphate, and glycerol on response variables. These controlled variables were further optimized using BBD matrix at N = 46 by second-order polynomial equation. The fermentation medium of pH 6.5 constituting yeast extract (0.5% w/v), magnesium sulphate (0.1% w/v), and glycerol (1.5% v/v), being further incubated at 30 °C showed enhanced anti-tubercular activity (98.7%) and approximately 4 fold increment in the bacterial biomass yield (8.3 mg/mL) with respect to traditional OFAT method. Three-dimensional response plots of the quadratic model showed interdependent interaction between the significant variables. In conclusion, the present study revealed the first report on the optimization of anti-tubercular activity and biomass of S. hominis via Taguchi OA as well as BBD design, and thus, paved a path for its proficient applications in pharmaceutical industries as dynamic mycobactericidal agent in future. Copyright © 2018

  17. CMOS gate array characterization procedures

    Spratt, James P.

    1993-09-01

    Present procedures are inadequate for characterizing the radiation hardness of gate array product lines prior to personalization because the selection of circuits to be used, from among all those available in the manufacturer's circuit library, is usually uncontrolled. (Some circuits are fundamentally more radiation resistant than others.) In such cases, differences in hardness can result between different designs of the same logic function. Hardness also varies because many gate arrays feature large custom-designed megacells (e.g., microprocessors and random access memories-MicroP's and RAM's). As a result, different product lines cannot be compared equally. A characterization strategy is needed, along with standardized test vehicle(s), methodology, and conditions, so that users can make informed judgments on which gate arrays are best suited for their needs. The program described developed preferred procedures for the radiation characterization of gate arrays, including a gate array evaluation test vehicle, featuring a canary circuit, designed to define the speed versus hardness envelope of the gate array. A multiplier was chosen for this role, and a baseline multiplier architecture is suggested that could be incorporated into an existing standard evaluation circuit chip.

  18. Multi-kW solar arrays for Earth orbit applications

    1985-01-01

    The multi-kW solar array program is concerned with developing the technology required to enable the design of solar arrays required to power the missions of the 1990's. The present effort required the design of a modular solar array panel consisting of superstrate modules interconnected to provide the structural support for the solar cells. The effort was divided into two tasks: (1) superstrate solar array panel design, and (2) superstrate solar array panel-to-panel design. The primary objective was to systematically investigate critical areas of the transparent superstrate solar array and evaluate the flight capabilities of this low cost approach.

  19. Optically Controlled Phased Array Antenna

    Garafalo, David

    1998-01-01

    .... The antenna is a 3-foot by 9 foot phased array capable of a scan angle of 120 degrees. The antenna was designed to be conformal to the cargo door of a large aircraft and is designed to operate in the frequency range of 830 - 1400 MHz with a 30...

  20. Copy number alterations in small intestinal neuroendocrine tumors determined by array comparative genomic hybridization

    Hashemi, Jamileh; Fotouhi, Omid; Sulaiman, Luqman; Kjellman, Magnus; Höög, Anders; Zedenius, Jan; Larsson, Catharina

    2013-01-01

    Small intestinal neuroendocrine tumors (SI-NETs) are typically slow-growing tumors that have metastasized already at the time of diagnosis. The purpose of the present study was to further refine and define regions of recurrent copy number (CN) alterations (CNA) in SI-NETs. Genome-wide CNAs was determined by applying array CGH (a-CGH) on SI-NETs including 18 primary tumors and 12 metastases. Quantitative PCR analysis (qPCR) was used to confirm CNAs detected by a-CGH as well as to detect CNAs in an extended panel of SI-NETs. Unsupervised hierarchical clustering was used to detect tumor groups with similar patterns of chromosomal alterations based on recurrent regions of CN loss or gain. The log rank test was used to calculate overall survival. Mann–Whitney U test or Fisher’s exact test were used to evaluate associations between tumor groups and recurrent CNAs or clinical parameters. The most frequent abnormality was loss of chromosome 18 observed in 70% of the cases. CN losses were also frequently found of chromosomes 11 (23%), 16 (20%), and 9 (20%), with regions of recurrent CN loss identified in 11q23.1-qter, 16q12.2-qter, 9pter-p13.2 and 9p13.1-11.2. Gains were most frequently detected in chromosomes 14 (43%), 20 (37%), 4 (27%), and 5 (23%) with recurrent regions of CN gain located to 14q11.2, 14q32.2-32.31, 20pter-p11.21, 20q11.1-11.21, 20q12-qter, 4 and 5. qPCR analysis confirmed most CNAs detected by a-CGH as well as revealed CNAs in an extended panel of SI-NETs. Unsupervised hierarchical clustering of recurrent regions of CNAs revealed two separate tumor groups and 5 chromosomal clusters. Loss of chromosomes 18, 16 and 11 and again of chromosome 20 were found in both tumor groups. Tumor group II was enriched for alterations in chromosome cluster-d, including gain of chromosomes 4, 5, 7, 14 and gain of 20 in chromosome cluster-b. Gain in 20pter-p11.21 was associated with short survival. Statistically significant differences were observed between primary

  1. Development of phased-array ultrasonic testing probe

    Kawanami, Seiichi; Kurokawa, Masaaki; Taniguchi, Masaru; Tada, Yoshihisa

    2001-01-01

    Phased-array ultrasonic testing was developed for nondestructive evaluation of power plants. Phased-array UT scans and focuses an ultrasonic beam to inspect areas difficult to inspect by conventional UT. We developed a highly sensitive piezoelectric composite, and designed optimized phased-array UT probes. We are applying our phased-array UT to different areas of power plants. (author)

  2. New Tools for Embryo Selection: Comprehensive Chromosome Screening by Array Comparative Genomic Hybridization

    Lorena Rodrigo

    2014-01-01

    Full Text Available The objective of this study was to evaluate the usefulness of comprehensive chromosome screening (CCS using array comparative genomic hybridization (aCGH. The study included 1420 CCS cycles for recurrent miscarriage (n=203; repetitive implantation failure (n=188; severe male factor (n=116; previous trisomic pregnancy (n=33; and advanced maternal age (n=880. CCS was performed in cycles with fresh oocytes and embryos (n=774; mixed cycles with fresh and vitrified oocytes (n=320; mixed cycles with fresh and vitrified day-2 embryos (n=235; and mixed cycles with fresh and vitrified day-3 embryos (n=91. Day-3 embryo biopsy was performed and analyzed by aCGH followed by day-5 embryo transfer. Consistent implantation (range: 40.5–54.2% and pregnancy rates per transfer (range: 46.0–62.9% were obtained for all the indications and independently of the origin of the oocytes or embryos. However, a lower delivery rate per cycle was achieved in women aged over 40 years (18.1% due to the higher percentage of aneuploid embryos (85.3% and lower number of cycles with at least one euploid embryo available per transfer (40.3%. We concluded that aneuploidy is one of the major factors which affect embryo implantation.

  3. High-power, ultralow-mass solar arrays: FY-77 solar arrays technology readiness assessment report, volume 2

    Costogue, E. N.; Young, L. E.; Brandhorst, H. W., Jr.

    1978-01-01

    Development efforts are reported in detail for: (1) a lightweight solar array system for solar electric propulsion; (2) a high efficiency thin silicon solar cell; (3) conceptual design of 200 W/kg solar arrays; (4) fluorocarbon encapsulation for silicon solar cell array; and (5) technology assessment of concentrator solar arrays.

  4. An aCGH classifier derived from BRCA1-mutated breast cancer and benefit of high-dose platinum-based chemotherapy in HER2-negative breast cancer patients

    Vollebergh, M. A.; Lips, E. H.; Nederlof, P. M.; Wessels, L. F. A.; Schmidt, M. K.; van Beers, E. H.; Cornelissen, S.; Holtkamp, M.; Froklage, F. E.; de Vries, E. G. E.; Schrama, J. G.; Wesseling, J.; van de Vijver, M. J.; van Tinteren, H.; de Bruin, Michiel; Hauptmann, M.; Rodenhuis, S.; Linn, S. C.

    2011-01-01

    Breast cancer cells deficient for BRCA1 are hypersensitive to agents inducing DNA double-strand breaks (DSB), such as bifunctional alkylators and platinum agents. Earlier, we had developed a comparative genomic hybridisation (CGH) classifier based on BRCA1-mutated breast cancers. We hypothesised

  5. The surface detector array of the Telescope Array experiment

    Abu-Zayyad, T. [University of Utah, High Energy Astrophysics Institute, Salt Lake City, Utah (United States); Aida, R. [University of Yamanashi, Interdisciplinary Graduate School of Medicine and Engineering, Kofu, Yamanashi (Japan); Allen, M.; Anderson, R. [University of Utah, High Energy Astrophysics Institute, Salt Lake City, Utah (United States); Azuma, R. [Tokyo Institute of Technology, Meguro, Tokyo (Japan); Barcikowski, E.; Belz, J.W.; Bergman, D.R.; Blake, S.A.; Cady, R. [University of Utah, High Energy Astrophysics Institute, Salt Lake City, Utah (United States); Cheon, B.G. [Hanyang University, Seongdong-gu, Seoul (Korea, Republic of); Chiba, J. [Tokyo University of Science, Noda, Chiba (Japan); Chikawa, M. [Kinki University, Higashi Osaka, Osaka (Japan); Cho, E.J. [Hanyang University, Seongdong-gu, Seoul (Korea, Republic of); Cho, W.R. [Yonsei University, Seodaemun-gu, Seoul (Korea, Republic of); Fujii, H. [Institute of Particle and Nuclear Studies, KEK, Tsukuba, Ibaraki (Japan); Fujii, T. [Osaka City University, Osaka, Osaka (Japan); Fukuda, T. [Tokyo Institute of Technology, Meguro, Tokyo (Japan); Fukushima, M. [Institute for Cosmic Ray Research, University of Tokyo, Kashiwa, Chiba (Japan); University of Tokyo, Institute for the Physics and Mathematics of the Universe, Kashiwa, Chiba (Japan); Gorbunov, D. [Institute for Nuclear Research of the Russian Academy of Sciences, Moscow (Russian Federation); and others

    2012-10-11

    The Telescope Array (TA) experiment, located in the western desert of Utah, USA, is designed for the observation of extensive air showers from extremely high energy cosmic rays. The experiment has a surface detector array surrounded by three fluorescence detectors to enable simultaneous detection of shower particles at ground level and fluorescence photons along the shower track. The TA surface detectors and fluorescence detectors started full hybrid observation in March, 2008. In this article we describe the design and technical features of the TA surface detector.

  6. The surface detector array of the Telescope Array experiment

    Abu-Zayyad, T.; Aida, R.; Allen, M.; Anderson, R.; Azuma, R.; Barcikowski, E.; Belz, J.W.; Bergman, D.R.; Blake, S.A.; Cady, R.; Cheon, B.G.; Chiba, J.; Chikawa, M.; Cho, E.J.; Cho, W.R.; Fujii, H.; Fujii, T.; Fukuda, T.; Fukushima, M.; Gorbunov, D.

    2012-01-01

    The Telescope Array (TA) experiment, located in the western desert of Utah, USA, is designed for the observation of extensive air showers from extremely high energy cosmic rays. The experiment has a surface detector array surrounded by three fluorescence detectors to enable simultaneous detection of shower particles at ground level and fluorescence photons along the shower track. The TA surface detectors and fluorescence detectors started full hybrid observation in March, 2008. In this article we describe the design and technical features of the TA surface detector.

  7. Coupling in reflector arrays

    Appel-Hansen, Jørgen

    1968-01-01

    In order to reduce the space occupied by a reflector array, it is desirable to arrange the array antennas as close to each other as possible; however, in this case coupling between the array antennas will reduce the reflecting properties of the reflector array. The purpose of the present communic......In order to reduce the space occupied by a reflector array, it is desirable to arrange the array antennas as close to each other as possible; however, in this case coupling between the array antennas will reduce the reflecting properties of the reflector array. The purpose of the present...

  8. Ferrite LTCC based phased array antennas

    Ghaffar, Farhan A.

    2016-11-02

    Two phased array antennas realized in multilayer ferrite LTCC technology are presented in this paper. The use of embedded bias windings in these designs allows the negation of external magnets which are conventionally employed with bulk ferrite medium. This reduces the required magnetostatic field strength by 90% as compared to the traditional designs. The phase shifters are implemented using the SIW technology. One of the designs is operated in the half mode waveguide topology while the other design is based on standard full mode waveguide operation. The two phase shifter designs are integrated with two element patch antenna array and slotted SIW array respectively. The array designs demonstrate a beam steering of 30° and ±19° respectively for a current excitation of 200 mA. The designs, due to their small factor can be easily integrated in modern communication systems which is not possible in the case of bulk ferrite based designs.

  9. ESPRIT And Uniform Linear Arrays

    Roy, R. H.; Goldburg, M.; Ottersten, B. E.; Swindlehurst, A. L.; Viberg, M.; Kailath, T.

    1989-11-01

    Abstract ¬â€?ESPRIT is a recently developed and patented technique for high-resolution estimation of signal parameters. It exploits an invariance structure designed into the sensor array to achieve a reduction in computational requirements of many orders of magnitude over previous techniques such as MUSIC, Burg's MEM, and Capon's ML, and in addition achieves performance improvement as measured by parameter estimate error variance. It is also manifestly more robust with respect to sensor errors (e.g. gain, phase, and location errors) than other methods as well. Whereas ESPRIT only requires that the sensor array possess a single invariance best visualized by considering two identical but other-wise arbitrary arrays of sensors displaced (but not rotated) with respect to each other, many arrays currently in use in various applications are uniform linear arrays of identical sensor elements. Phased array radars are commonplace in high-resolution direction finding systems, and uniform tapped delay lines (i.e., constant rate A/D converters) are the rule rather than the exception in digital signal processing systems. Such arrays possess many invariances, and are amenable to other types of analysis, which is one of the main reasons such structures are so prevalent. Recent developments in high-resolution algorithms of the signal/noise subspace genre including total least squares (TLS) ESPRIT applied to uniform linear arrays are summarized. ESPRIT is also shown to be a generalization of the root-MUSIC algorithm (applicable only to the case of uniform linear arrays of omni-directional sensors and unimodular cisoids). Comparisons with various estimator bounds, including CramerRao bounds, are presented.

  10. Seismometer array station processors

    Key, F.A.; Lea, T.G.; Douglas, A.

    1977-01-01

    A description is given of the design, construction and initial testing of two types of Seismometer Array Station Processor (SASP), one to work with data stored on magnetic tape in analogue form, the other with data in digital form. The purpose of a SASP is to detect the short period P waves recorded by a UK-type array of 20 seismometers and to edit these on to a a digital library tape or disc. The edited data are then processed to obtain a rough location for the source and to produce seismograms (after optimum processing) for analysis by a seismologist. SASPs are an important component in the scheme for monitoring underground explosions advocated by the UK in the Conference of the Committee on Disarmament. With digital input a SASP can operate at 30 times real time using a linear detection process and at 20 times real time using the log detector of Weichert. Although the log detector is slower, it has the advantage over the linear detector that signals with lower signal-to-noise ratio can be detected and spurious large amplitudes are less likely to produce a detection. It is recommended, therefore, that where possible array data should be recorded in digital form for input to a SASP and that the log detector of Weichert be used. Trial runs show that a SASP is capable of detecting signals down to signal-to-noise ratios of about two with very few false detections, and at mid-continental array sites it should be capable of detecting most, if not all, the signals with magnitude above msub(b) 4.5; the UK argues that, given a suitable network, it is realistic to hope that sources of this magnitude and above can be detected and identified by seismological means alone. (author)

  11. Airborne electronically steerable phased array

    1972-01-01

    The results are presented of the second stage of a program for the design and development of a phased array capable of simultaneous and separate transmission and reception of radio frequency signals at S-band frequencies. The design goals of this stage were the development of three major areas of interest required for the final prototype model. These areas are the construction and testing of the low-weight, full-scale 128-element array of antenna elements, the development of the RF manifold feed system, and the construction and testing of a working module containing diplexer and transmit and receive circuits.

  12. Optical Epitaxial Growth of Gold Nanoparticle Arrays.

    Huang, Ningfeng; Martínez, Luis Javier; Jaquay, Eric; Nakano, Aiichiro; Povinelli, Michelle L

    2015-09-09

    We use an optical analogue of epitaxial growth to assemble gold nanoparticles into 2D arrays. Particles are attracted to a growth template via optical forces and interact through optical binding. Competition between effects determines the final particle arrangements. We use a Monte Carlo model to design a template that favors growth of hexagonal particle arrays. We experimentally demonstrate growth of a highly stable array of 50 gold particles with 200 nm diameter, spaced by 1.1 μm.

  13. ELECTROMAGNETIC SCATTERING AND ANTENNA TECHNOLOGY (EMSAT) Task Order 0003: Design of a Circularly Polarized, 20 60 GHZ Active Phased Array for Wide Angle Scanning

    2017-08-08

    previously published linear -to-circular polarizers. This is because the first sheet has a low inductance in the -direction, which acts as a wire-grid...GHZ Active Phased Array for Wide Angle Scanning Carl R. Pfeiffer Defense Engineering Corporation Boris Tomasic Multispectral Sensing and...GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 62204F/61102F 6. AUTHOR(S) Carl R. Pfeiffer (Defense Engineering Corporation) Boris Tomasic (AFRL

  14. Extended Monopole antenna Array with individual Shield (EMAS) coil: An improved monopole antenna design for brain imaging at 7 tesla MRI.

    Woo, Myung-Kyun; Hong, Suk-Min; Lee, Jongho; Kang, Chang-Ki; Park, Sung-Yeon; Son, Young-Don; Kim, Young-Bo; Cho, Zang-Hee

    2016-06-01

    To propose a new Extended Monopole antenna Array with individual Shields (EMAS) coil that improves the B1 field coverage and uniformity along the z-direction. To increase the spatial coverage of Monopole antenna Array (MA) coil, each monopole antenna was shielded and extended in length. Performance of this new coil, which is referred to as EMAS coil, was compared with the original MA coil and an Extended Monopole antenna Array coil with no shield (EMA). For comparison, flip angle, signal-to-noise ratio (SNR), and receive sensitivity maps were measured at multiple regions of interest (ROIs) in the brain. The EMAS coil demonstrated substantially larger flip angle and receive sensitivity than the MA and EMA coils in the inferior aspect of the brain. In the brainstem ROI, for example, the flip angle in the EMAS coil was increased by 45.5% (or 60.0%) and the receive sensitivity was increased by 26.9% (or 14.9%), resulting in an SNR gain of 84.8% (or 76.3%) when compared with the MA coil (or EMA). The EMAS coil provided 25.7% (or 24.4%) more uniform B1+ field distribution compared with the MA (or EMA) coil in sagittal. The EMAS coil successfully extended the imaging volume in lower part of the brain. Magn Reson Med 75:2566-2572, 2016. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.

  15. Design and preliminary testing of a MEMS microphone phased array for aeroacoustic testing of a small-scale wind turbine airfoil

    Bale, A.; Orlando, S.; Johnson, D. [Waterloo Univ., ON (Canada). Wind Energy Group

    2010-07-01

    One of the barriers preventing the widespread utilization of wind turbines is the audible sound that they produce. Developing quieter wind turbines will increase the amount of available land onto which wind farms can be built. Noise emissions from wind turbines can be attributed to the aerodynamic effects between the turbine blades and the air surrounding them. A dominant source of these aeroacoustic emissions from wind turbines is known to originate at the trailing edges of the airfoils. This study investigated the flow physics of noise generation in an effort to reduce noise from small-scale wind turbine airfoils. The trailing edge noise was studied on scale-models in wind tunnels and applied to full scale conditions. Microphone phased arrays are popular research tools in wind tunnel aeroacoustic studies because they can measure and locate noise sources. However, large arrays of microphones can be prohibitively expensive. This paper presented preliminary testing of micro-electrical mechanical system (MEMS) microphones in phased arrays for aeroacoustic testing on a small wind turbine airfoil. Preliminary results showed that MEMS microphones are an acceptable low-cost alternative to costly condenser microphones. 19 refs., 1 tab., 11 figs.

  16. Remedy and Recontamination Assessment Array

    2017-03-01

    instruments pre-installed from the R/V Ecos during the April 22, 2016 event. .................................................................... 38...Environmental Security Technology Certification Program IBI Index of Benthic Integrity ISMA In situ Microcosm Arrays HOC Hydrophobic Organic Compound...system was successfully designed and constructed based on the goal of providing an integrated technology for assessing the effectiveness of

  17. Refracting surface plasmon polaritons with nanoparticle arrays

    Radko, I.P.; Evlyukhin, A.B.; Boltasseva, Alexandra

    2008-01-01

    Refraction of surface plasmon polaritons (SPPs) by various structures formed by a 100-nm-period square lattice of gold nanoparticles on top of a gold film is studied by leakage radiation microscopy. SPP refraction by a triangular-shaped nanoparticle array indicates that the SPP effective refractive...... to design nanoparticle arrays for specific applications requiring in-plane SPP manipulation....

  18. Array processors based on Gaussian fraction-free method

    Peng, S; Sedukhin, S [Aizu Univ., Aizuwakamatsu, Fukushima (Japan); Sedukhin, I

    1998-03-01

    The design of algorithmic array processors for solving linear systems of equations using fraction-free Gaussian elimination method is presented. The design is based on a formal approach which constructs a family of planar array processors systematically. These array processors are synthesized and analyzed. It is shown that some array processors are optimal in the framework of linear allocation of computations and in terms of number of processing elements and computing time. (author)

  19. A transmit/receive radiofrequency array for imaging the carotid arteries at 7 Tesla: coil design and first in vivo results.

    Kraff, Oliver; Bitz, Andreas K; Breyer, Tobias; Kruszona, Stefan; Maderwald, Stefan; Brote, Irina; Gizewski, Elke R; Ladd, Mark E; Quick, Harald H

    2011-04-01

    To develop a transmit/receive radiofrequency (RF) array for magnetic resonance imaging (MRI) of the carotid arteries at 7 T. The prototype is characterized in numerical simulations and bench measurements, and the feasibility of plaque imaging at 7 T is demonstrated in first in vivo images. The RF phased array coil consists of 8 surface loop coils. To allow imaging of both sides of the neck, the RF array is divided into 2 coil clusters, each with 4 overlapping loop elements. For safety validation, numerical computations of the RF field distribution and the corresponding specific absorption rate were performed on the basis of a heterogeneous human body model. To validate the coil model, maps of the transmit B1(+) field were compared between simulation and measurement. In vivo images of a healthy volunteer and a patient (ulcerating plaque and a 50% stenosis of the right internal carotid artery) were acquired using a 3-dimensional FLASH sequence with a high isotropic spatial resolution of 0.54 mm as well as using pulse-triggered proton density (PD)/T2-weighted turbo spin echo sequences. Measurements of the S-parameters yielded a reflection and isolation of the coil elements of better than -18 and -13 dB, respectively. Measurements of the g-factor indicated good image quality for parallel imaging acceleration factors up to 2.4. A similar distribution and a very good match of the absolute values were found between the measured and simulated B1(+) transmit RF field for the validation of the coil model. In vivo images revealed good signal excitation of both sides of the neck and a high vessel-to-background image contrast for the noncontrast-enhanced 3-dimensional FLASH sequence. Imaging at 7 T could depict the extent of stenosis, and revealed the disruption and ulcer of the plaque. This study demonstrates that 2 four-channel transmit/receive RF arrays for each side of the neck is a suitable concept for in vivo MRI of the carotid arteries at 7 Tesla. Further studies are

  20. Characterization of photovoltaic array performance: an overview

    Ross, Jr., R. G.

    1986-09-15

    Characterization of the electrical performance of a photovoltaic array can take many forms depending on the end use of the data. Typical uses include buyer-seller negotiations, system performance prediction, and performance measurement. Buyer-seller negotiations may deal with specifying the size (power) of an array to be purchased under some standard reporting conditions, and may treat the warranty conditions governing allowable degradation of this performance with time. System design, on the other hand, requires prediction of performance under varying field conditions, not standard reporting conditions, and must include the non-ideal realities of operating systems: array shadowing, steep angles of incidence, soiling, and array-load energy utilization. Typical uses of predicted array performance include array sizing tradeoffs, tracking-pointing comparisons, load-array interface analyses and system economic evaluations. The third use, performance measurement, refers to the characterization of an as-built array as opposed to prediction of the performance of an array to be built. This may be done to assess actual array performance or to measure performance degradation over time.

  1. Improved linear pyroelectric IR detector arrays

    Twiney, R.C.; Robinson, M.K.; Porter, S.G.

    1987-01-01

    Good agreement has been found between theoretical models and measured performance for a range of array geometries. A 64-element 80 x 140-micron element array with integral MOSFET IC buffer preamplifiers shows improved source voltage uniformity, a J-FET buffered array, and low-frequency specific detectivity (SD) of 1.7 x 10 to the 8th cm sq rt Hz/W at 40 Hz. The MOSFET array shows reduced degradation of SD at high temperatures, retaining an SD of not less than 1 x 10 to the 8th cm sq rt Hz/W at +70 C across much of the band. A 64-element array has been designed using onboard multiplexers, thus greatly reducing the connections needed to run the array

  2. Optimal design of tilt carrier frequency computer-generated holograms to measure aspherics.

    Peng, Jiantao; Chen, Zhe; Zhang, Xingxiang; Fu, Tianjiao; Ren, Jianyue

    2015-08-20

    Computer-generated holograms (CGHs) provide an approach to high-precision metrology of aspherics. A CGH is designed under the trade-off among size, mapping distortion, and line spacing. This paper describes an optimal design method based on the parametric model for tilt carrier frequency CGHs placed outside the interferometer focus points. Under the condition of retaining an admissible size and a tolerable mapping distortion, the optimal design method has two advantages: (1) separating the parasitic diffraction orders to improve the contrast of the interferograms and (2) achieving the largest line spacing to minimize sensitivity to fabrication errors. This optimal design method is applicable to common concave aspherical surfaces and illustrated with CGH design examples.

  3. Design of Pd/PANI/Pd sandwich-structured nanotube array catalysts with special shape effects and synergistic effects for ethanol electrooxidation.

    Wang, An-Liang; Xu, Han; Feng, Jin-Xian; Ding, Liang-Xin; Tong, Ye-Xiang; Li, Gao-Ren

    2013-07-24

    Low cost, high activity, and long-term durability are the main requirements for commercializing fuel cell electrocatalysts. Despite tremendous efforts, developing non-Pt anode electrocatalysts with high activity and long-term durability at low cost remains a significant technical challenge. Here we report a new type of hybrid Pd/PANI/Pd sandwich-structured nanotube array (SNTA) to exploit shape effects and synergistic effects of Pd-PANI composites for the oxidation of small organic molecules for direct alcohol fuel cells. These synthesized Pd/PANI/Pd SNTAs exhibit significantly improved electrocatalytic activity and durability compared with Pd NTAs and commercial Pd/C catalysts. The unique SNTAs provide fast transport and short diffusion paths for electroactive species and high utilization rate of catalysts. Besides the merits of nanotube arrays, the improved electrocatalytic activity and durability are especially attributed to the special Pd/PANI/Pd sandwich-like nanostructures, which results in electron delocalization between Pd d orbitals and PANI π-conjugated ligands and in electron transfer from Pd to PANI.

  4. Design and implementation of an array of micro-electrochemical detectors for two-dimensional liquid chromatography--proof of principle.

    Abia, Jude A; Putnam, Joel; Mriziq, Khaled; Guiochon, Georges A

    2010-03-05

    Simultaneous two-dimensional liquid chromatography (2D-LC) is an implementation of two-dimensional liquid chromatography which has the potential to provide very fast, yet highly efficient separations. It is based on the use of time x space and space x space separation systems. The basic principle of this instrument has been validated long ago by the success of two-dimensional thin layer chromatography. The construction of a pressurized wide and flat column (100 mm x 100 mm x 1 mm) operated under an inlet pressure of up to 50 bar was described previously. However, to become a modern analytical method, simultaneous 2D-LC requires the development of detectors suitable for the monitoring of the composition of the eluent of this pressurized planar, wide column. An array of five equidistant micro-electrochemical sensors was built for this purpose and tested. Each sensor is a three-electrode system, with the working electrode being a 25 microm polished platinum micro-electrode. The auxiliary electrode is a thin platinum wire and the reference electrode an Ag/AgCl (3M sat. KCl) electrode. In this first implementation, proof of principle is demonstrated, but the final instrument will require a much larger array. 2010 Elsevier B.V. All rights reserved.

  5. Challenges, constraints, and results of lens design for 17 micron-bolometer focal plane arrays in 8-12 micron waveband

    Schuster, Norbert; Franks, John

    2011-06-01

    In the 8-12 micron waveband Focal Plane Arrays (FPA) are available with a 17 micron pixel pitch in different arrays sizes (e.g. 512 x 480 pixels and 320 x 240 pixels) and with excellent electrical properties. Many applications become possible using this new type of IR-detector which will become the future standard in uncooled technology. Lenses with an f-number faster than f/1.5 minimize the diffraction impact on the spatial resolution and guarantee a high thermal resolution for uncooled cameras. Both effects will be quantified. The distinction between Traditional f-number (TF) and Radiometric f-number (RF) is discussed. Lenses with different focal lengths are required for applications in a variety of markets. They are classified by their Horizontal field of view (HFOV). Respecting the requirements for high volume markets, several two lens solutions will be discussed. A commonly accepted parameter of spatial resolution is the Modulation Transfer Function (MTF)-value at the Nyquist frequency of the detector (here 30cy/mm). This parameter of resolution will be presented versus field of view. Wide Angle and Super Wide Angle lenses are susceptible to low relative illumination in the corner of the detector. Measures to reduce this drop to an acceptable value are presented.

  6. Computationally assisted screening and design of cell-interactive peptides by a cell-based assay using peptide arrays and a fuzzy neural network algorithm.

    Kaga, Chiaki; Okochi, Mina; Tomita, Yasuyuki; Kato, Ryuji; Honda, Hiroyuki

    2008-03-01

    We developed a method of effective peptide screening that combines experiments and computational analysis. The method is based on the concept that screening efficiency can be enhanced from even limited data by use of a model derived from computational analysis that serves as a guide to screening and combining the model with subsequent repeated experiments. Here we focus on cell-adhesion peptides as a model application of this peptide-screening strategy. Cell-adhesion peptides were screened by use of a cell-based assay of a peptide array. Starting with the screening data obtained from a limited, random 5-mer library (643 sequences), a rule regarding structural characteristics of cell-adhesion peptides was extracted by fuzzy neural network (FNN) analysis. According to this rule, peptides with unfavored residues in certain positions that led to inefficient binding were eliminated from the random sequences. In the restricted, second random library (273 sequences), the yield of cell-adhesion peptides having an adhesion rate more than 1.5-fold to that of the basal array support was significantly high (31%) compared with the unrestricted random library (20%). In the restricted third library (50 sequences), the yield of cell-adhesion peptides increased to 84%. We conclude that a repeated cycle of experiments screening limited numbers of peptides can be assisted by the rule-extracting feature of FNN.

  7. Preliminary analysis of numerical chromosome abnormalities in reciprocal and Robertsonian translocation preimplantation genetic diagnosis cases with 24-chromosomal analysis with an aCGH/SNP microarray.

    Xie, Yanxin; Xu, Yanwen; Wang, Jing; Miao, Benyu; Zeng, Yanhong; Ding, Chenhui; Gao, Jun; Zhou, Canquan

    2018-01-01

    The aim of this study was to determine whether an interchromosomal effect (ICE) occurred in embryos obtained from reciprocal translocation (rcp) and Robertsonian translocation (RT) carriers who were following a preimplantation genetic diagnosis (PGD) with whole chromosome screening with an aCGH and SNP microarray. We also analyzed the chromosomal numerical abnormalities in embryos with aneuploidy in parental chromosomes that were not involved with a translocation and balanced in involved parental translocation chromosomes. This retrospective study included 832 embryos obtained from rcp carriers and 382 embryos from RT carriers that were biopsied in 139 PGD cycles. The control group involved embryos obtained from age-matched patient karyotypes who were undergoing preimplantation genetic screening (PGS) with non-translocation, and 579 embryos were analyzed in the control group. A single blastomere at the cleavage stage or trophectoderm from a blastocyst was biopsied, and 24-chromosomal analysis with an aCGH/SNP microarray was conducted using the PGD/PGS protocols. Statistical analyses were implemented on the incidences of cumulative aneuploidy rates between the translocation carriers and the control group. Reliable results were obtained from 138 couples, among whom only one patient was a balanced rcp or RT translocation carrier, undergoing PGD testing in our center from January 2012 to June 2014. For day 3 embryos, the aneuploidy rates were 50.7% for rcp carriers and 49.1% for RT carriers, compared with the control group, with 44.8% at a maternal age < 36 years. When the maternal age was ≥ 36 years, the aneuploidy rates were increased to 61.1% for rcp carriers, 56.7% for RT carriers, and 60.3% for the control group. There were no significant differences. In day 5 embryos, the aneuploidy rates were 24.5% for rcp carriers and 34.9% for RT carriers, compared with the control group with 53.6% at a maternal age < 36 years. When the maternal age was ≥ 36

  8. Application of the micro-array comparative genomic hybridization technology in preimplantation genetic diagnosis%Array-CGH技术在胚胎植入前遗传学诊断中的应用进展

    韩丹; 陈大蔚; 曹云霞; 周平

    2015-01-01

    As a new kind high-throughput genomics technology, micro array-based comparative genomic hybridization (aCGH) has brought the huge change for molecular biology and medical research. Because of the detection range covers the whole genome, high efficiency, easy operation etc, aCGH has been widely used in many areas of human genetic disease diagnosis, tumor genomics, systems biology and prenatal diagnosis. Human preimplantation genetic diagnosis (PGD) is an important part of assisted reproductive technology, with the development of molecular genetics technology, its application range is continuously widening. Based on aCGH technology in PGD for embryonic whole genome screening for aneuploidy and structural abnormalities, human PGD/human preimplantation genetic screening (PGS) implantation rate and clinical pregnancy rate have improved significantly. In this article, we discussed the advantages, disadvantages and prospects of aCGH in prenatal diagnosis.%微阵列比较基因组杂交(aCGH)作为一种新兴的高通量检测技术,给分子生物学及医学研究带来了巨大变化,因其检测范围覆盖全基因组、高效率、操作简便等特点,在人类遗传疾病诊断,肿瘤基因组学,系统生物学研究及产前诊断中已有了广泛应用。植入前遗传学诊断(PGD)是辅助生殖技术的重要组成部分,随着分子遗传学技术的发展,其应用范围也不断拓宽。基于aCGH技术在PGD中对胚胎全染色体组非整倍体及结构异常的筛查,PGD/植入前遗传学筛查(PGS)胚胎植入率和临床妊娠率均有显著提高,本文就aCGH技术在胚胎植入前遗传学诊断中的应用进行综述。

  9. Selecting Sums in Arrays

    Brodal, Gerth Stølting; Jørgensen, Allan Grønlund

    2008-01-01

    In an array of n numbers each of the \\binomn2+nUnknown control sequence '\\binom' contiguous subarrays define a sum. In this paper we focus on algorithms for selecting and reporting maximal sums from an array of numbers. First, we consider the problem of reporting k subarrays inducing the k largest...... sums among all subarrays of length at least l and at most u. For this problem we design an optimal O(n + k) time algorithm. Secondly, we consider the problem of selecting a subarray storing the k’th largest sum. For this problem we prove a time bound of Θ(n · max {1,log(k/n)}) by describing...... an algorithm with this running time and by proving a matching lower bound. Finally, we combine the ideas and obtain an O(n· max {1,log(k/n)}) time algorithm that selects a subarray storing the k’th largest sum among all subarrays of length at least l and at most u....

  10. Topology optimization of Halbach magnet arrays using isoparametric projection

    Lee, Jaewook; Nomura, Tsuyoshi; Dede, Ercan M.

    2017-01-01

    Highlights: • Design method of Halbach magnet array is proposed using topology optimization. • Magnet strength and direction are simultaneously optimized by isoparametric projection. • For manufacturing feasibility of magnet, penalization and extrusion schemes are proposed. • Design results of circular shaped Halbach arrays are provided. • Halbach arrays in linear actuator are optimized to maximize magnetic force. - Abstract: Topology optimization using isoparametric projection for the design of permanent magnet patterns in Halbach arrays is proposed. Based on isoparametric shape functions used in the finite element analysis, the permanent magnet strength and magnetization directions in a Halbach array are simultaneously optimized for a given design goal. To achieve fabrication feasibility of a designed Halbach magnet array, two design schemes are combined with the isoparametric projection method. First, a penalization scheme is proposed for designing the permanent magnets to have discrete magnetization direction angles. Second, an extrusion scheme is proposed for the shape regularization of the permanent magnet segments. As a result, the method systematically finds the optimal permanent magnet patterns of a Halbach array considering manufacturing feasibility. In two numerical examples, a circular shaped permanent magnet Halbach array is designed to minimize the magnitude of the magnetic flux density and to maximize the upward direction magnetic flux density inside the magnet array. Logical extension of the method to the design of permanent magnet arrays in linear actuators is provided, where the design goal is to maximize the actuator magnetic force.

  11. Topology optimization of Halbach magnet arrays using isoparametric projection

    Lee, Jaewook, E-mail: jaewooklee@gist.ac.kr [School of Mechanical Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju, 61005 (Korea, Republic of); Nomura, Tsuyoshi [Toyota Central R& D Labs., Inc., 41-1 Yokomichi, Aichi 480-1192 (Japan); Toyota Research Institute of North America, 1555 Woodridge Avenue, Ann Arbor, MI 48105 (United States); Dede, Ercan M. [Toyota Research Institute of North America, 1555 Woodridge Avenue, Ann Arbor, MI 48105 (United States)

    2017-06-15

    Highlights: • Design method of Halbach magnet array is proposed using topology optimization. • Magnet strength and direction are simultaneously optimized by isoparametric projection. • For manufacturing feasibility of magnet, penalization and extrusion schemes are proposed. • Design results of circular shaped Halbach arrays are provided. • Halbach arrays in linear actuator are optimized to maximize magnetic force. - Abstract: Topology optimization using isoparametric projection for the design of permanent magnet patterns in Halbach arrays is proposed. Based on isoparametric shape functions used in the finite element analysis, the permanent magnet strength and magnetization directions in a Halbach array are simultaneously optimized for a given design goal. To achieve fabrication feasibility of a designed Halbach magnet array, two design schemes are combined with the isoparametric projection method. First, a penalization scheme is proposed for designing the permanent magnets to have discrete magnetization direction angles. Second, an extrusion scheme is proposed for the shape regularization of the permanent magnet segments. As a result, the method systematically finds the optimal permanent magnet patterns of a Halbach array considering manufacturing feasibility. In two numerical examples, a circular shaped permanent magnet Halbach array is designed to minimize the magnitude of the magnetic flux density and to maximize the upward direction magnetic flux density inside the magnet array. Logical extension of the method to the design of permanent magnet arrays in linear actuators is provided, where the design goal is to maximize the actuator magnetic force.

  12. Shielding in ungated field emitter arrays

    Harris, J. R. [U.S. Navy Reserve, Navy Operational Support Center New Orleans, New Orleans, Louisiana 70143 (United States); Jensen, K. L. [Code 6854, Naval Research Laboratory, Washington, D.C. 20375 (United States); Shiffler, D. A. [Directed Energy Directorate, Air Force Research Laboratory, Albuquerque, New Mexico 87117 (United States); Petillo, J. J. [Leidos, Billerica, Massachusetts 01821 (United States)

    2015-05-18

    Cathodes consisting of arrays of high aspect ratio field emitters are of great interest as sources of electron beams for vacuum electronic devices. The desire for high currents and current densities drives the cathode designer towards a denser array, but for ungated emitters, denser arrays also lead to increased shielding, in which the field enhancement factor β of each emitter is reduced due to the presence of the other emitters in the array. To facilitate the study of these arrays, we have developed a method for modeling high aspect ratio emitters using tapered dipole line charges. This method can be used to investigate proximity effects from similar emitters an arbitrary distance away and is much less computationally demanding than competing simulation approaches. Here, we introduce this method and use it to study shielding as a function of array geometry. Emitters with aspect ratios of 10{sup 2}–10{sup 4} are modeled, and the shielding-induced reduction in β is considered as a function of tip-to-tip spacing for emitter pairs and for large arrays with triangular and square unit cells. Shielding is found to be negligible when the emitter spacing is greater than the emitter height for the two-emitter array, or about 2.5 times the emitter height in the large arrays, in agreement with previously published results. Because the onset of shielding occurs at virtually the same emitter spacing in the square and triangular arrays, the triangular array is preferred for its higher emitter density at a given emitter spacing. The primary contribution to shielding in large arrays is found to come from emitters within a distance of three times the unit cell spacing for both square and triangular arrays.

  13. Optimal shortening of uniform covering arrays.

    Jose Torres-Jimenez

    Full Text Available Software test suites based on the concept of interaction testing are very useful for testing software components in an economical way. Test suites of this kind may be created using mathematical objects called covering arrays. A covering array, denoted by CA(N; t, k, v, is an N × k array over [Formula: see text] with the property that every N × t sub-array covers all t-tuples of [Formula: see text] at least once. Covering arrays can be used to test systems in which failures occur as a result of interactions among components or subsystems. They are often used in areas such as hardware Trojan detection, software testing, and network design. Because system testing is expensive, it is critical to reduce the amount of testing required. This paper addresses the Optimal Shortening of Covering ARrays (OSCAR problem, an optimization problem whose objective is to construct, from an existing covering array matrix of uniform level, an array with dimensions of (N - δ × (k - Δ such that the number of missing t-tuples is minimized. Two applications of the OSCAR problem are (a to produce smaller covering arrays from larger ones and (b to obtain quasi-covering arrays (covering arrays in which the number of missing t-tuples is small to be used as input to a meta-heuristic algorithm that produces covering arrays. In addition, it is proven that the OSCAR problem is NP-complete, and twelve different algorithms are proposed to solve it. An experiment was performed on 62 problem instances, and the results demonstrate the effectiveness of solving the OSCAR problem to facilitate the construction of new covering arrays.

  14. Design Methodology of an Equalizer for Unipolar Non Return to Zero Binary Signals in the Presence of Additive White Gaussian Noise Using a Time Delay Neural Network on a Field Programmable Gate Array

    Pérez Suárez, Santiago T.; Travieso González, Carlos M.; Alonso Hernández, Jesús B.

    2013-01-01

    This article presents a design methodology for designing an artificial neural network as an equalizer for a binary signal. Firstly, the system is modelled in floating point format using Matlab. Afterward, the design is described for a Field Programmable Gate Array (FPGA) using fixed point format. The FPGA design is based on the System Generator from Xilinx, which is a design tool over Simulink of Matlab. System Generator allows one to design in a fast and flexible way. It uses low level details of the circuits and the functionality of the system can be fully tested. System Generator can be used to check the architecture and to analyse the effect of the number of bits on the system performance. Finally the System Generator design is compiled for the Xilinx Integrated System Environment (ISE) and the system is described using a hardware description language. In ISE the circuits are managed with high level details and physical performances are obtained. In the Conclusions section, some modifications are proposed to improve the methodology and to ensure portability across FPGA manufacturers.

  15. Design Methodology of an Equalizer for Unipolar Non Return to Zero Binary Signals in the Presence of Additive White Gaussian Noise Using a Time Delay Neural Network on a Field Programmable Gate Array

    Santiago T. Pérez Suárez

    2013-12-01

    Full Text Available This article presents a design methodology for designing an artificial neural network as an equalizer for a binary signal. Firstly, the system is modelled in floating point format using Matlab. Afterward, the design is described for a Field Programmable Gate Array (FPGA using fixed point format. The FPGA design is based on the System Generator from Xilinx, which is a design tool over Simulink of Matlab. System Generator allows one to design in a fast and flexible way. It uses low level details of the circuits and the functionality of the system can be fully tested. System Generator can be used to check the architecture and to analyse the effect of the number of bits on the system performance. Finally the System Generator design is compiled for the Xilinx Integrated System Environment (ISE and the system is described using a hardware description language. In ISE the circuits are managed with high level details and physical performances are obtained. In the Conclusions section, some modifications are proposed to improve the methodology and to ensure portability across FPGA manufacturers.

  16. Fiber Laser Array

    Simpson, Thomas

    2002-01-01

    ...., field-dependent, loss within the coupled laser array. During this program, Jaycor focused on the construction and use of an experimental apparatus that can be used to investigate the coherent combination of an array of fiber lasers...

  17. Dynamics of Josephson junction arrays

    Hadley, P.

    1989-01-01

    The dynamics of Josephson junction arrays is a topic that lies at the intersection of the fields of nonlinear dynamics and Josephson junction technology. The series arrays considered here consist of several rapidly oscillating Josephson junctions where each junction is coupled equally to every other junction. The purpose of this study is to understand phaselocking and other cooperative dynamics of this system. Previously, little was known about high dimensional nonlinear systems of this sort. Numerical simulations are used to study the dynamics of these arrays. Three distinct types of periodic solutions to the array equations were observed as well as period doubled and chaotic solutions. One of the periodic solutions is the symmetric, in-phase solution where all of the junctions oscillate identically. The other two periodic solutions are symmetry-broken solutions where all of the junction do not oscillate identically. The symmetry-broken solutions are highly degenerate. As many as (N - 1) stable solutions can coexist for an array of N junctions. Understanding the stability of these several solutions and the transitions among them is vital to the design of useful devices

  18. Array based characterization of a terminal deletion involving chromosome subband 15q26.2: an emerging syndrome associated with growth retardation, cardiac defects and developmental delay

    Björkhem Gudrun

    2008-01-01

    Full Text Available Abstract Background Subtelomeric regions are gene rich and deletions in these chromosomal segments have been demonstrated to account for approximately 2.5% of patients displaying mental retardation with or without association of dysmorphic features. However, cases that report de novo terminal deletions on chromosome arm 15q are rare. Methods In this study we present the first example of a detailed molecular genetic mapping of a de novo deletion in involving 15q26.2-qter, caused by the formation of a dicentric chromosome 15, using metaphase FISH and tiling resolution (32 k genome-wide array-based comparative genomic hybridization (CGH. Results After an initial characterization of the dicentric chromosome by metaphase FISH, array CGH analysis mapped the terminal deletion to encompass a 6.48 megabase (Mb region, ranging from 93.86–100.34 Mb on chromosome 15. Conclusion In conclusion, we present an additional case to the growing family of reported cases with 15q26-deletion, thoroughly characterized at the molecular cytogenetic level. In the deleted regions, four candidate genes responsible for the phenotype of the patient could be delineated: IGFR1, MEF2A, CHSY1, and TM2D3. Further characterization of additional patients harboring similar 15q-aberrations might hopefully in the future lead to the description of a clear cut clinically recognizable syndrome.

  19. Design and performance of modularized NaI(Tl) detectors with rectangular crystal elements: An array of 49 and the Crystal Box

    Wilson, S.L.; Hofstadter, R.; Hughes, E.B.; Lin, Y.C.; Parks, R.; Rolfe, J.; Bolton, R.D.; Bowman, J.D.; Cooper, M.D.; Hoffman, C.M.; Hogan, G.E.; Mariam, F.G.; Mischke, R.E.; Nagle, D.E.; Piilonen, L.E.; Sandberg, V.D.; Sanders, G.H.; Werbeck, R.; Williams, R.A.; Frank, J.S.; Hallin, A.L.; Matis, H.S.; Sennhauser, U.; Wright, S.C.

    1988-01-01

    An array of 49 NaI(Tl) modules each 20 inch in depth and 2.5 inch x 2.5 inch in cross section has been constructed and its properties, especially energy resolution, explored for positrons in the range 20 MeV - 18 GeV. A subsequent much larger detector, the Crystal Box, has also been constructed from 396 modules of the same cross section, but mostly 12 inch in depth, and operated as a γ-ray and positron detector in a search for rare muon decays. The calibration procedure used for the Crystal Box and its characteristic resolutions in energy, impact point and time are described. (orig.)

  20. Non-Contact Sensor for Long-Term Continuous Vital Signs Monitoring: A Review on Intelligent Phased-Array Doppler Sensor Design.

    Hall, Travis; Lie, Donald Y C; Nguyen, Tam Q; Mayeda, Jill C; Lie, Paul E; Lopez, Jerry; Banister, Ron E

    2017-11-15

    It has been the dream of many scientists and engineers to realize a non-contact remote sensing system that can perform continuous, accurate and long-term monitoring of human vital signs as we have seen in many Sci-Fi movies. Having an intelligible sensor system that can measure and record key vital signs (such as heart rates and respiration rates) remotely and continuously without touching the patients, for example, can be an invaluable tool for physicians who need to make rapid life-and-death decisions. Such a sensor system can also effectively help physicians and patients making better informed decisions when patients' long-term vital signs data is available. Therefore, there has been a lot of research activities on developing a non-contact sensor system that can monitor a patient's vital signs and quickly transmit the information to healthcare professionals. Doppler-based radio-frequency (RF) non-contact vital signs (NCVS) monitoring system are particularly attractive for long term vital signs monitoring because there are no wires, electrodes, wearable devices, nor any contact-based sensors involved so the subjects may not be even aware of the ubiquitous monitoring. In this paper, we will provide a brief review on some latest development on NCVS sensors and compare them against a few novel and intelligent phased-array Doppler-based RF NCVS biosensors we have built in our labs. Some of our NCVS sensor tests were performed within a clutter-free anechoic chamber to mitigate the environmental clutters, while most tests were conducted within the typical Herman-Miller type office cubicle setting to mimic a more practical monitoring environment. Additionally, we will show the measurement data to demonstrate the feasibility of long-term NCVS monitoring. The measured data strongly suggests that our latest phased array NCVS system should be able to perform long-term vital signs monitoring intelligently and robustly, especially for situations where the subject is sleeping

  1. Configuration Considerations for Low Frequency Arrays

    Lonsdale, C. J.

    2005-12-01

    The advance of digital signal processing capabilities has spurred a new effort to exploit the lowest radio frequencies observable from the ground, from ˜10 MHz to a few hundred MHz. Multiple scientifically and technically complementary instruments are planned, including the Mileura Widefield Array (MWA) in the 80-300 MHz range, and the Long Wavelength Array (LWA) in the 20-80 MHz range. The latter instrument will target relatively high angular resolution, and baselines up to a few hundred km. An important practical question for the design of such an array is how to distribute the collecting area on the ground. The answer to this question profoundly affects both cost and performance. In this contribution, the factors which determine the anticipated performance of any such array are examined, paying particular attention to the viability and accuracy of array calibration. It is argued that due to the severity of ionospheric effects in particular, it will be difficult or impossible to achieve routine, high dynamic range imaging with a geographically large low frequency array, unless a large number of physically separate array stations is built. This conclusion is general, is based on the need for adequate sampling of ionospheric irregularities, and is independent of the calibration algorithms and techniques that might be employed. It is further argued that array configuration figures of merit that are traditionally used for higher frequency arrays are inappropriate, and a different set of criteria are proposed.

  2. Power Efficient Design of DisplayPort (7.0) Using Low-voltage differential signaling IO Standard Via UltraScale Field Programming Gate Arrays

    Das, Bhagwan; Abdullah, M.F.L.; Hussain, Dil muhammed Akbar

    2017-01-01

    Port (7.0) need be reduced. In this paper, a power efficient design for DisplayPort (7.0) is proposed using LVDS IO Standard. The proposed design is tested for different frequencies; 500 MHz, 700 MHz, 1.0 GHz, and 1.6 GHz. The design is implemented using vhdl in UltraScale FPGA. It is determined...... the designed vhdl based design of DisplayPort (7.0) can reduced 92% using LVDS IO Standard for all frequencies; 500 MHz, 700 MHz, 1.0 GHz, and 1.6 GHz, compared to vhdl based design of DisplayPort (7.0) without using IO Standard. The proposed design of vhdl based design of DisplayPort (7.0) using LVDS IO...... Standard offers no power consumption for DisplayPort (7.0) in standby mode. The vhdl based design of DisplayPort (7.0) using LVDS IO Standard will be helpful to process the high resolution video at low power consumption....

  3. Linearly tapered slot antenna circular array for mobile communications

    Simons, Rainee N.; Kelly, Eron; Lee, Richard Q.; Taub, Susan R.

    1993-01-01

    The design, fabrication and testing of a conformal K-band circular array is presented. The array consists of sixteen linearly tapered slot antennas (LTSA). It is fed by a 1:16 microstrip line power splitter via electromagnetic coupling. The array has an omni-directional pattern in the azimuth plane. In the elevation plane the beam is displaced above the horizon.

  4. Finiteness effects in wideband connected arrays: Analytical models to highlight the effects of the loading impedances

    Neto, A.; Cavallo, D.; Gerini, G.

    2011-01-01

    Most phased arrays are designed using infinite array theory, which does not account for edge effects. However, this approximation might not be adequate for the design of wideband arrays, for which truncation effects are more significant than in traditional narrow-band arrays. In particular, edge

  5. Photovoltaic Array Space Power flight experiment plus diagnostics (PASP+) modules

    Cooley, W.T.; Adams, S.F.; Reinhardt, K.C.; Piszczor, M.F.

    1992-01-01

    The Photovoltaic Array Space Power Plus Diagnostics flight experiment (PASP+) subsumes twelve solar array modules which represent the state of the art in the space photovoltaic array industry. Each of the twelve modules individually feature specific photovoltaic technologies such as advanced semiconductor materials, multi-bandgap structures, lightweight array designs, advanced interconnect technologies, or concentrator array designs. This paper will describe each module in detail including the configuration, components, materials, anticipated on orbit performance, and some of the aspects of each array technology. The layout of each module and the photovoltaic cell or array cross section will be presented graphically. A discussion on the environmental constraints and materials selection will be included as well as a delineation of the differences between the modules and the baseline array configuration in its intended application

  6. Non-Contact Sensor for Long-Term Continuous Vital Signs Monitoring: A Review on Intelligent Phased-Array Doppler Sensor Design

    Hall, Travis; Nguyen, Tam Q.; Mayeda, Jill C.; Lie, Paul E.; Lopez, Jerry; Banister, Ron E.

    2017-01-01

    It has been the dream of many scientists and engineers to realize a non-contact remote sensing system that can perform continuous, accurate and long-term monitoring of human vital signs as we have seen in many Sci-Fi movies. Having an intelligible sensor system that can measure and record key vital signs (such as heart rates and respiration rates) remotely and continuously without touching the patients, for example, can be an invaluable tool for physicians who need to make rapid life-and-death decisions. Such a sensor system can also effectively help physicians and patients making better informed decisions when patients’ long-term vital signs data is available. Therefore, there has been a lot of research activities on developing a non-contact sensor system that can monitor a patient’s vital signs and quickly transmit the information to healthcare professionals. Doppler-based radio-frequency (RF) non-contact vital signs (NCVS) monitoring system are particularly attractive for long term vital signs monitoring because there are no wires, electrodes, wearable devices, nor any contact-based sensors involved so the subjects may not be even aware of the ubiquitous monitoring. In this paper, we will provide a brief review on some latest development on NCVS sensors and compare them against a few novel and intelligent phased-array Doppler-based RF NCVS biosensors we have built in our labs. Some of our NCVS sensor tests were performed within a clutter-free anechoic chamber to mitigate the environmental clutters, while most tests were conducted within the typical Herman-Miller type office cubicle setting to mimic a more practical monitoring environment. Additionally, we will show the measurement data to demonstrate the feasibility of long-term NCVS monitoring. The measured data strongly suggests that our latest phased array NCVS system should be able to perform long-term vital signs monitoring intelligently and robustly, especially for situations where the subject is

  7. Non-Contact Sensor for Long-Term Continuous Vital Signs Monitoring: A Review on Intelligent Phased-Array Doppler Sensor Design

    Travis Hall

    2017-11-01

    Full Text Available It has been the dream of many scientists and engineers to realize a non-contact remote sensing system that can perform continuous, accurate and long-term monitoring of human vital signs as we have seen in many Sci-Fi movies. Having an intelligible sensor system that can measure and record key vital signs (such as heart rates and respiration rates remotely and continuously without touching the patients, for example, can be an invaluable tool for physicians who need to make rapid life-and-death decisions. Such a sensor system can also effectively help physicians and patients making better informed decisions when patients’ long-term vital signs data is available. Therefore, there has been a lot of research activities on developing a non-contact sensor system that can monitor a patient’s vital signs and quickly transmit the information to healthcare professionals. Doppler-based radio-frequency (RF non-contact vital signs (NCVS monitoring system are particularly attractive for long term vital signs monitoring because there are no wires, electrodes, wearable devices, nor any contact-based sensors involved so the subjects may not be even aware of the ubiquitous monitoring. In this paper, we will provide a brief review on some latest development on NCVS sensors and compare them against a few novel and intelligent phased-array Doppler-based RF NCVS biosensors we have built in our labs. Some of our NCVS sensor tests were performed within a clutter-free anechoic chamber to mitigate the environmental clutters, while most tests were conducted within the typical Herman-Miller type office cubicle setting to mimic a more practical monitoring environment. Additionally, we will show the measurement data to demonstrate the feasibility of long-term NCVS monitoring. The measured data strongly suggests that our latest phased array NCVS system should be able to perform long-term vital signs monitoring intelligently and robustly, especially for situations where the

  8. Application of Chemical Doping and Architectural Design Principles To Fabricate Nanowire Co2Ni3ZnO8 Arrays for Aqueous Asymmetric Supercapacitors.

    Liu, Qi; Yang, Bin; Liu, Jingyuan; Yuan, Yi; Zhang, Hongsen; Liu, Lianhe; Wang, Jun; Li, Rumin

    2016-08-10

    Electrode materials derived from transition metal oxides have a serious problem of low electron transfer rate, which restricts their practical application. However, chemically doped graphene transforms the chemical bonding configuration to enhance electron transfer rate and, therefore, facilitates the successful fabrication of Co2Ni3ZnO8 nanowire arrays. In addition, the Co2Ni3ZnO8 electrode materials, considered as Ni and Zn ions doped into Co3O4, have a high electron transfer rate and electrochemical response capability, because the doping increases the degree of crystal defect and reaction of Co/Ni ions with the electrolyte. Hence, the Co2Ni3ZnO8 electrode exhibits a high rate property and excellent electrochemical cycle stability, as determined by electrochemical analysis of the relationship between specific capacitance, IR drop, Coulomb efficiency, and different current densities. From the results of a three-electrode system of electrochemical measurement, the Co2Ni3ZnO8 electrode demonstrates a specific capacitance of 1115 F g(-1) and retains 89.9% capacitance after 2000 cycles at a current density of 4 A g(-1). The energy density of the asymmetric supercapacitor (AC//Co2Ni3ZnO8) is 54.04 W h kg(-1) at the power density of 3200 W kg(-1).

  9. Design of a high-gain laser diode-array pumped Nd:YAG Alternating Precessive Slab Amplifier (APS-Amplifier)

    Coyle, D. Barry

    1991-01-01

    In the design of space qualifiable laser systems for ranging and altimetry, such as NASA's Geodynamic Laser Ranging System (GLRS), the transmitter must be kept small, powerful yet efficient, and must consist of as few components as possible. A novel preamplifier design is examined which requires no external beam steering optics, yielding a compact component with simple alignment procedures. The gains achieved are comparable to multipass zigzag amplifiers using two or more sets of external optics for extra passes through the amplifying medium.

  10. Wind loads on flat plate photovoltaic array fields

    Miller, R. D.; Zimmerman, D. K.

    1981-01-01

    The results of an experimental analysis (boundary layer wind tunnel test) of the aerodynamic forces resulting from winds acting on flat plate photovoltaic arrays are presented. Local pressure coefficient distributions and normal force coefficients on the arrays are shown and compared to theoretical results. Parameters that were varied when determining the aerodynamic forces included tilt angle, array separation, ground clearance, protective wind barriers, and the effect of the wind velocity profile. Recommended design wind forces and pressures are presented, which envelop the test results for winds perpendicular to the array's longitudinal axis. This wind direction produces the maximum wind loads on the arrays except at the array edge where oblique winds produce larger edge pressure loads. The arrays located at the outer boundary of an array field have a protective influence on the interior arrays of the field. A significant decrease of the array wind loads were recorded in the wind tunnel test on array panels located behind a fence and/or interior to the array field compared to the arrays on the boundary and unprotected from the wind. The magnitude of this decrease was the same whether caused by a fence or upwind arrays.

  11. Hybrid Arrays for Chemical Sensing

    Kramer, Kirsten E.; Rose-Pehrsson, Susan L.; Johnson, Kevin J.; Minor, Christian P.

    intelligence and robotics, all share the same essential data fusion challenges. The design of a hybrid sensor array should draw on this extended body of knowledge. In this chapter, various techniques for data preprocessing, feature extraction, feature selection, and modeling of sensor data will be introduced and illustrated with data fusion approaches that have been implemented in applications involving data from hybrid arrays. The example systems discussed in this chapter involve the development of prototype sensor networks for damage control event detection aboard US Navy vessels and the development of analysis algorithms to combine multiple sensing techniques for enhanced remote detection of unexploded ordnance (UXO) in both ground surveys and wide area assessments.

  12. Degree-of-Freedom Strengthened Cascade Array for DOD-DOA Estimation in MIMO Array Systems.

    Yao, Bobin; Dong, Zhi; Zhang, Weile; Wang, Wei; Wu, Qisheng

    2018-05-14

    In spatial spectrum estimation, difference co-array can provide extra degrees-of-freedom (DOFs) for promoting parameter identifiability and parameter estimation accuracy. For the sake of acquiring as more DOFs as possible with a given number of physical sensors, we herein design a novel sensor array geometry named cascade array. This structure is generated by systematically connecting a uniform linear array (ULA) and a non-uniform linear array, and can provide more DOFs than some exist array structures but less than the upper-bound indicated by minimum redundant array (MRA). We further apply this cascade array into multiple input multiple output (MIMO) array systems, and propose a novel joint direction of departure (DOD) and direction of arrival (DOA) estimation algorithm, which is based on a reduced-dimensional weighted subspace fitting technique. The algorithm is angle auto-paired and computationally efficient. Theoretical analysis and numerical simulations prove the advantages and effectiveness of the proposed array structure and the related algorithm.

  13. Carbon nanotube nanoelectrode arrays

    Ren, Zhifeng; Lin, Yuehe; Yantasee, Wassana; Liu, Guodong; Lu, Fang; Tu, Yi

    2008-11-18

    The present invention relates to microelectode arrays (MEAs), and more particularly to carbon nanotube nanoelectrode arrays (CNT-NEAs) for chemical and biological sensing, and methods of use. A nanoelectrode array includes a carbon nanotube material comprising an array of substantially linear carbon nanotubes each having a proximal end and a distal end, the proximal end of the carbon nanotubes are attached to a catalyst substrate material so as to form the array with a pre-determined site density, wherein the carbon nanotubes are aligned with respect to one another within the array; an electrically insulating layer on the surface of the carbon nanotube material, whereby the distal end of the carbon nanotubes extend beyond the electrically insulating layer; a second adhesive electrically insulating layer on the surface of the electrically insulating layer, whereby the distal end of the carbon nanotubes extend beyond the second adhesive electrically insulating layer; and a metal wire attached to the catalyst substrate material.

  14. Solar array flight dynamic experiment

    Schock, Richard W.

    1987-01-01

    The purpose of the Solar Array Flight Dynamic Experiment (SAFDE) is to demonstrate the feasibility of on-orbit measurement and ground processing of large space structures' dynamic characteristics. Test definition or verification provides the dynamic characteristic accuracy required for control systems use. An illumination/measurement system was developed to fly on space shuttle flight STS-41D. The system was designed to dynamically evaluate a large solar array called the Solar Array Flight Experiment (SAFE) that had been scheduled for this flight. The SAFDE system consisted of a set of laser diode illuminators, retroreflective targets, an intelligent star tracker receiver and the associated equipment to power, condition, and record the results. In six tests on STS-41D, data was successfully acquired from 18 retroreflector targets and ground processed, post flight, to define the solar array's dynamic characteristic. The flight experiment proved the viability of on-orbit test definition of large space structures dynamic characteristics. Future large space structures controllability should be greatly enhanced by this capability.

  15. Multiwall carbon nanotube microcavity arrays

    Ahmed, Rajib; Butt, Haider, E-mail: h.butt@bham.ac.uk [Nanotechnology Laboratory, School of Mechanical Engineering, University of Birmingham, Birmingham B15 2TT (United Kingdom); Rifat, Ahmmed A. [Integrated Lightwave Research Group, Department of Electrical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603 (Malaysia); Yetisen, Ali K.; Yun, Seok Hyun [Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 65 Landsdowne Street, Cambridge, Massachusetts 02139 (United States); Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 (United States); Dai, Qing [National Center for Nanoscience and Technology, Beijing 100190 (China)

    2016-03-21

    Periodic highly dense multi-wall carbon nanotube (MWCNT) arrays can act as photonic materials exhibiting band gaps in the visible regime and beyond terahertz range. MWCNT arrays in square arrangement for nanoscale lattice constants can be configured as a microcavity with predictable resonance frequencies. Here, computational analyses of compact square microcavities (≈0.8 × 0.8 μm{sup 2}) in MWCNT arrays were demonstrated to obtain enhanced quality factors (≈170–180) and narrow-band resonance peaks. Cavity resonances were rationally designed and optimized (nanotube geometry and cavity size) with finite element method. Series (1 × 2 and 1 × 3) and parallel (2 × 1 and 3 × 1) combinations of microcavities were modeled and resonance modes were analyzed. Higher order MWCNT microcavities showed enhanced resonance modes, which were red shifted with increasing Q-factors. Parallel microcavity geometries were also optimized to obtain narrow-band tunable filtering in low-loss communication windows (810, 1336, and 1558 nm). Compact series and parallel MWCNT microcavity arrays may have applications in optical filters and miniaturized optical communication devices.

  16. Josephson junction arrays

    Bindslev Hansen, J.; Lindelof, P.E.

    1985-01-01

    In this review we intend to cover recent work involving arrays of Josephson junctions. The work on such arrays falls naturally into three main areas of interest: 1. Technical applications of Josephson junction arrays for high-frequency devices. 2. Experimental studies of 2-D model systems (Kosterlitz-Thouless phase transition, commensurate-incommensurate transition in frustrated (flux) lattices). 3. Investigations of phenomena associated with non-equilibrium superconductivity in and around Josephson junctions (with high current density). (orig./BUD)

  17. Phased-array radars

    Brookner, E.

    1985-02-01

    The operating principles, technology, and applications of phased-array radars are reviewed and illustrated with diagrams and photographs. Consideration is given to the antenna elements, circuitry for time delays, phase shifters, pulse coding and compression, and hybrid radars combining phased arrays with lenses to alter the beam characteristics. The capabilities and typical hardware of phased arrays are shown using the US military systems COBRA DANE and PAVE PAWS as examples.

  18. Characterization of canine osteosarcoma by array comparative genomic hybridization and RT-qPCR: signatures of genomic imbalance in canine osteosarcoma parallel the human counterpart.

    Angstadt, Andrea Y; Motsinger-Reif, Alison; Thomas, Rachael; Kisseberth, William C; Guillermo Couto, C; Duval, Dawn L; Nielsen, Dahlia M; Modiano, Jaime F; Breen, Matthew

    2011-11-01

    Osteosarcoma (OS) is the most commonly diagnosed malignant bone tumor in humans and dogs, characterized in both species by extremely complex karyotypes exhibiting high frequencies of genomic imbalance. Evaluation of genomic signatures in human OS using array comparative genomic hybridization (aCGH) has assisted in uncovering genetic mechanisms that result in disease phenotype. Previous low-resolution (10-20 Mb) aCGH analysis of canine OS identified a wide range of recurrent DNA copy number aberrations, indicating extensive genomic instability. In this study, we profiled 123 canine OS tumors by 1 Mb-resolution aCGH to generate a dataset for direct comparison with current data for human OS, concluding that several high frequency aberrations in canine and human OS are orthologous. To ensure complete coverage of gene annotation, we identified the human refseq genes that map to these orthologous aberrant dog regions and found several candidate genes warranting evaluation for OS involvement. Specifically, subsequenct FISH and qRT-PCR analysis of RUNX2, TUSC3, and PTEN indicated that expression levels correlated with genomic copy number status, showcasing RUNX2 as an OS associated gene and TUSC3 as a possible tumor suppressor candidate. Together these data demonstrate the ability of genomic comparative oncology to identify genetic abberations which may be important for OS progression. Large scale screening of genomic imbalance in canine OS further validates the use of the dog as a suitable model for human cancers, supporting the idea that dysregulation discovered in canine cancers will provide an avenue for complementary study in human counterparts. Copyright © 2011 Wiley-Liss, Inc.

  19. Storage array reflection considerations

    Haire, M.J.; Jordan, W.C.; Taylor, R.G.

    1997-01-01

    The assumptions used for reflection conditions of single containers are fairly well established and consistently applied throughout the industry in nuclear criticality safety evaluations. Containers are usually considered to be either fully water reflected (i.e., surrounded by 6 to 12 in. of water) for safety calculations or reflected by 1 in. of water for nominal (structural material and air) conditions. Tables and figures are usually available for performing comparative evaluations of containers under various loading conditions. Reflection considerations used for evaluating the safety of storage arrays of fissile material are not as well established. When evaluating arrays, it has become more common for analysts to use calculations to demonstrate the safety of the array configuration. In performing these calculations, the analyst has considerable freedom concerning the assumptions made for modeling the reflection of the array. Considerations are given for the physical layout of the array with little or no discussion (or demonstration) of what conditions are bounded by the assumed reflection conditions. For example, an array may be generically evaluated by placing it in a corner of a room in which the opposing walls are far away. Typically, it is believed that complete flooding of the room is incredible, so the array is evaluated for various levels of water mist interspersed among array containers. This paper discusses some assumptions that are made regarding storage array reflection

  20. The EUROBALL array

    Rossi Alvarez, C.

    1998-01-01

    The quality of the multidetector array EUROBALL is described, with emphasis on the history and formal organization of the related European collaboration. The detector layout is presented together with the electronics and Data Acquisition capabilities. The status of the instrument, its performances and the main features of some recently developed ancillary detectors will also be described. The EUROBALL array is operational in Legnaro National Laboratory (Italy) since April 1997 and is expected to run up to November 1998. The array represents a significant improvement in detector efficiency and sensitivity with respect to the previous generation of multidetector arrays