WorldWideScience

Sample records for ceu infekciju izraistju

  1. From Undergraduate UCNs: my CEU-launched neutron career

    Science.gov (United States)

    Blatnik, Marie

    2017-09-01

    The CEU changed my life, introducing me to the Ultracold Neutron (UCN) experiments that brought me to Caltech as a UCN collaborator at Los Alamos and Oak Ridge National Labs. This story includes undergraduate-level overviews of UCN production, the Los Alamos Neutron Science Center beta decay correlation experiments, and the Oak Ridge Spallation Neutron Source nEDM (neutron Electric Dipole Moment) experiment. Progress with Metglas shielding and Cavello multiplier high voltage apparatus development for the nEDM apparatus development will also be presented.

  2. Global similarity with local differences in linkage disequilibrium between the Dutch and HapMap-CEU populations.

    Science.gov (United States)

    Pardo, Luba; Bochdanovits, Zoltán; de Geus, Eco; Hottenga, Jouke J; Sullivan, Patrick; Posthuma, Danielle; Penninx, Brenda W J H; Boomsma, Dorret; Heutink, Peter

    2009-06-01

    The HapMap project has facilitated the selection of tagging single nucleotide polymorphisms (tagSNPs) for genome-wide association studies (GWAS) under the assumption that linkage disequilibrium (LD) in the HapMap populations is similar to the populations under investigation. Earlier reports support this assumption, although in most of these studies only a few loci were evaluated. We compared pair-wise LD and LD block structure across autosomes between the Dutch population and the CEU-HapMap reference panel. The impact of sampling distribution on the estimation of LD blocks was studied by bootstrapping. A high Pearson correlation (genome-wide; 0.93) between pair-wise r(2) for the Dutch and the CEU populations was found, indicating that tagSNPs from the CEU-HapMap panel capture common variation in the Dutch population. However, some genomic regions exhibited, significantly lower correlation than the genome-wide estimate. This might decrease the validity of HapMap tagSNPs in these regions and the power of GWAS. The LD block structure differed considerably between the Dutch and CEU-HapMap populations. This was not explained by demographic differences between the CEU and Dutch samples, as testing for population stratification was not significant. We also found that sampling variation had a large effect on the estimation of LD blocks, as shown by the bootstrapping analysis. Thus, in small samples, most of the observed differences in LD blocks between populations are most likely the result of sampling variation. This poor concordance in LD block structure suggests that large samples are required for robust estimations of local LD block structure in populations.

  3. Analysis of genome-wide RNA-sequencing data suggests age of the CEPH/Utah (CEU) lymphoblastoid cell lines systematically biases gene expression profiles.

    Science.gov (United States)

    Yuan, Yuan; Tian, Lei; Lu, Dongsheng; Xu, Shuhua

    2015-01-22

    In human, Lymphoblastoid cell lines (LCLs) from the CEPH/CEU (Centre d'Etude du Polymorphisme Humain - Utah) family resource have been extensively used for examining the genetics of gene expression levels. However, we noted that CEU/CEPH cell lines were collected and transformed approximately thirty years ago, much earlier than the other cell lines from the pertaining individuals, which we suspected could potentially affect gene expression, data analysis and results interpretation. In this study, by analyzing RNA sequencing data of CEU and the other three European populations as well as an African population, we systematically examined and evaluated the potential confounding effect of LCL age on gene expression levels and patterns. Our results indicated that gene expression profiles of CEU samples have been biased by the older age of CEU cell lines. Interestingly, most of CEU-specific expressions are associated with functions related to cell proliferation, which are more likely due to older age of cell lines than intrinsic characters of the population. We suggested the results be carefully explained when CEU LCLs are used for transcriptomic data analysis in future studies.

  4. Estudios sobre la Defensa Nacional en las Universidades Públicas y Privadas: La perspectiva de la Universidad CEU-San Pablo

    Directory of Open Access Journals (Sweden)

    Seijas Candelas, Leopoldo

    2008-12-01

    Full Text Available The studies regarding the National Security and Defence within the Spanish university have been recently implemented. It has been certainly difficult to achieve that this concepts become mentioned in the lessons and that they contribute to create the called “Defence culture”. The university cannot ignore the reality of our society and the actual turbulent situations we are facing since the university should be the reflection and projection of the whole society. According to this, the San Pablo CEU University, aware of the importance of this knowledge area, has included this subject within Information Sciences studies.Los estudios sobre la seguridad y la defensa, en el ámbito universitario español, son de reciente implantación. Ha sido difícil que estos dos conceptos entren en las aulas y que contribuyan a lo que se conoce como “cultura de la defensa”. La universidad no puede ir contra la realidad de la sociedad y de las convulsiones de los tiempos actuales, porque en definitiva, ésta es reflejo y proyección de la sociedad total. En este sentido. la Universidad CEU-San Pablo, consciente de la importancia de esta área de conocimiento incorporó esta materia en sus planes de estudios en la carrera de Ciencias de la Información.

  5. Competencias profesionales y empleo en el futuro periodista: el caso de los estudiantes de Periodismo de la Universidad Cardenal Herrera-CEU

    Directory of Open Access Journals (Sweden)

    Javier Sierra Sánchez

    2010-12-01

    Full Text Available Presentamos un trabajo de investigación científica enfocado en dos aspectos: la adquisición de competencias profesionales en los alumnos de la licenciatura de Periodismo de la Universidad Cardenal Herrera-CEU y la importancia que otorgan a diferentes aspectos de un empleo. Éste último aspecto, partiendo del Informe Reflex de ANECA “El profesional flexible en la Sociedad del Conocimiento” establecemos una comparativa entre los resultados allí obtenidos y el caso particular de los alumnos de Periodismo de la Cardenal Herrera-CEU. Para descubrir estos aspectos presentamos un trabajo empírico para conocer en qué medida los alumnos de último curso de la licenciatura de la Universidad Cardenal Herrera-CEU han adquirido las competencias profesionales que se reflejan en el Libro Blanco de ANECA de los títulos de grado en Comunicación . Este estudio que presentamos nace con vocación de continuidad en un doble sentido: ser un indicador de la calidad de las enseñanzas que reciben los alumnos de Periodismo de la Universidad Cardenal Herrera-CEU y conocer en un futuro próximo (cuando salga la primera promoción de graduados si existe una diferencia significativa entre los alumnos que cursaron la licenciatura y los nuevos graduados fruto de la reforma universitaria. De este modo, podremos conocer hasta qué punto y qué grado se ha acometido en la Universidad Cardenal Herrera-CEU esa transformación que conlleva el Plan Bolonia en su sentido más profundo de la reforma.

  6. Interactions of the periplasmic binding protein CeuE with Fe(III) n-LICAM4- siderophore analogues of varied linker length

    Science.gov (United States)

    Wilde, Ellis J.; Hughes, Adam; Blagova, Elena V.; Moroz, Olga V.; Thomas, Ross P.; Turkenburg, Johan P.; Raines, Daniel J.; Duhme-Klair, Anne-Kathrin; Wilson, Keith S.

    2017-04-01

    Bacteria use siderophores to mediate the transport of essential Fe(III) into the cell. In Campylobacter jejuni the periplasmic binding protein CeuE, an integral part of the Fe(III) transport system, has adapted to bind tetradentate siderophores using a His and a Tyr side chain to complete the Fe(III) coordination. A series of tetradentate siderophore mimics was synthesized in which the length of the linker between the two iron-binding catecholamide units was increased from four carbon atoms (4-LICAM4-) to five, six and eight (5-, 6-, 8-LICAM4-, respectively). Co-crystal structures with CeuE showed that the inter-planar angles between the iron-binding catecholamide units in the 5-, 6- and 8-LICAM4- structures are very similar (111°, 110° and 110°) and allow for an optimum fit into the binding pocket of CeuE, the inter-planar angle in the structure of 4-LICAM4- is significantly smaller (97°) due to restrictions imposed by the shorter linker. Accordingly, the protein-binding affinity was found to be slightly higher for 5- compared to 4-LICAM4- but decreases for 6- and 8-LICAM4-. The optimum linker length of five matches that present in natural siderophores such as enterobactin and azotochelin. Site-directed mutagenesis was used to investigate the relative importance of the Fe(III)-coordinating residues H227 and Y288.

  7. Competencias profesionales y empleo en el futuro periodista: el caso de los estudiantes de periodismo de la Universidad San Pablo CEU

    Directory of Open Access Journals (Sweden)

    Javier Sierra Sánchez

    2012-04-01

    Full Text Available La Universidad española ha sufrido una gran transformación desde su adaptación al EEES. El Plan Bolonia propone un replanteamiento de la misión que debe cumplir la Universidad acercando dicha institución a los intereses y necesidades de la empresa y de la sociedad. Uno de los pilares básicos de estos cambios es el que hace referencia a la formación del alumno por competencias. Presentamos un trabajo de investigación científica enfocado en dos aspectos: la adquisición de competencias profesionales en los alumnos de la licenciatura de Periodismo de la Universidad San Pablo CEU y  la importancia que otorgan a diferentes aspectos de un empleo. Éste último aspecto, partiendo del Informe Réflex de ANECA “El profesional flexible en la Sociedad del Conocimiento” establecemos una comparativa entre los resultados allí obtenidos y el caso particular de los alumnos de Periodismo de la San Pablo CEU. Para descubrir estos aspectos presentamos un trabajo empírico para conocer en qué medida los alumnos de último curso de la licenciatura (que no de grado de la Universidad San Pablo CEU han adquirido las competencias profesionales que se reflejan en el Libro Blanco de ANECA de los títulos de grado en Comunicación.Este estudio que presentamos nace con vocación de continuidad en un doble sentido: ser un indicador de la calidad de las enseñanzas que reciben los alumnos de Periodismo de la San Pablo CEU y conocer en un futuro próximo (cuando salga la primera promoción de graduados si existe una diferencia significativa entre los alumnos que cursaron la licenciatura y los nuevos graduados fruto de la reforma universitaria. De este modo, podremos conocer hasta qué punto y qué grado se ha acometido en la Universidad San Pablo CEU esa transformación que conlleva el Plan Bolonia en su sentido más profundo de la reforma.

  8. Global similarity with local differences in linkage disequilibrium between the Dutch and HapMap–CEU populations

    NARCIS (Netherlands)

    Pardo, L.M.; Bochdanovits, Z.; de Geus, E.J.C.; Hottenga, J.J.; Sullivan, P.F.; Posthuma, D.; Penninx, B.W.J.H.; Boomsma, D.I.; Heutink, P.

    2009-01-01

    The HapMap project has facilitated the selection of tagging single nucleotide polymorphisms (tagSNPs) for genome-wide association studies (GWAS) under the assumption that linkage disequilibrium (LD) in the HapMap populations is similar to the populations under investigation. Earlier reports support

  9. Global similarity with local differences in linkage disequilibrium between the Dutch and HapMap-CEU populations

    NARCIS (Netherlands)

    Pardo, Luba; Bochdanovits, Zoltan; de Geus, Eco; Hottenga, Jouke J.; Sullivan, Patrick; Posthuma, Danielle; Penninx, Brenda W. J. H.; Boomsma, Dorret; Heutink, Peter

    The HapMap project has facilitated the selection of tagging single nucleotide polymorphisms (tagSNPs) for genome-wide association studies (GWAS) under the assumption that linkage disequilibrium (LD) in the HapMap populations is similar to the populations under investigation. Earlier reports support

  10. CEU.gif"/> The construction of work–life balance: The experience of Black employees in a call-centre environment

    OpenAIRE

    Sonia C.B. Potgieter; Antoni Barnard

    2010-01-01

    Orientation: Work–life balance, as a crucial aspect of employee and organisational wellness, remains an interesting field of research, especially due to the changing demographic employee profile.Research purpose: The objective of the study was to explore Black employees’ construction of work–life balance in a customer care environment.Motivation for the study: The conceptual debate regarding the construct of work–life balance in general as well as limited qualitative research with regard to B...

  11. Business Process Re-Engineering (BPR) of the Navy’s Information Professional (IP) Community’s Continuing Education Unit (CEU) Tracking Process

    Science.gov (United States)

    2006-03-01

    Microsoft Access and JET, applications with incompatible features, or large applications without database abstraction. ( Hillyer , 2005) With any...sure to see results of increased performance, cross-platform capability and open source. ( Hillyer , 2005) e. Oracle Oracle is one of the most widely...M. and Champy, J. (1993). Re-engineering the Corporation: A Manifesto for Business Revolution, New York: Harper Business Press, 1993. Hillyer

  12. Las tutorías en la formación académica y humana de los alumnos en la Universidad San Pablo CEU

    OpenAIRE

    Escribano Ródenas, María del Carmen; Fernández Barberis, Gabriela Mónica

    2008-01-01

    La función tutorial universitaria, en sus diversas modalidades, se concibe como la ayuda ofrecida al alumno, tanto en el plano académico como en el personal y en el profesional.Generalmente, se ha puesto mucho énfasis en la tutoría estrictamente académica, dejando de lado la tutoría exclusivamente personal, rasgo que caracteriza a unas pocas universidades españolas que la llevan realizando desde hace bastante tiempo.El objetivo del presente trabajo es poner de manifiesto la importancia del ré...

  13. Lighting measurement station: measurements of natural lighting for characterization of sky and zenith illuminance; Estacao de medicao de iluminacao: medicao de niveis de iluminacao natural para caracterizacao de iluminancias de ceu e luminancia de zenite

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Roberta Vieira Goncalves de [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Escola de Arquitetura. Dept. de Tecnologia da Arquitetura e do Urbanismo]. E-mail: roberta@arq.ufmg.br; Pereira, Fernando Oscar Ruttkay [Santa Catarina Univ., Florianopolis, SC (Brazil). Dept. de Arquitetura. Lab. de Conforto Ambiental]. E-mail: feco@arq.ufsc.br

    2002-07-01

    This paper describes the assembly of a standard station for measuring the day lighting for evaluation of the natural light availability. The mounting and data obtention form will also be described from a standard IDMP day lighting station for measuring the data from measuring zenith, horizontal, and vertical illuminance of the sky and vertical irradiance for the cardinal points.

  14. Formación de especialistas sanitarios (FIR) de Farmacia Industrial y Galénica de la Unidad Docente de la Universidad CEU San Pablo tras estancias en la industria farmacéutica de Madrid

    OpenAIRE

    Río, Luis Alberto del; Trives, Carmen; Salazar, Nuria

    2015-01-01

    El programa español de especialistas sanitarios en Farmacia Industrial y Galénica vía FIR garantiza, de acuerdo con los requisitos oficiales, que el especialista adquiere los conocimientos prácticos y competencias necesarias, con un alto grado de talento y aplicación industrial, en los diferentes campos del desarrollo, fabricación, calidad y regulación del medicamento en su aplicación industrial. Mediante el estudio, basado en una serie de encuestas de las dos últimas promociones de titula...

  15. Formación de especialistas sanitarios (FIR de Farmacia Industrial y Galénica de la Unidad Docente de la Universidad CEU San Pablo tras estancias en la industria farmacéutica de Madrid

    Directory of Open Access Journals (Sweden)

    Luis Alberto del Río

    2015-10-01

    La incorporación profesional de los farmacéuticos industriales demuestra una alta tasa de empleo dentro de la industria madrileña, incluso antes de terminar su período de formación en la mayoría de los casos. La formación recibida en la Escuela ha sido útil para conseguir empleo de forma rápida y con una responsabilidad relacionada con la formación recibida. Asimismo, se ha producido un aumento en la duración de las estancias en los laboratorios madrileños, lo que ha permitido a los titulados una mayor experiencia y vínculo profesional.

  16. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture Series All Training Information News & Events Press ...

  17. Tai Chi and Qi Gong for Health and Well-Being

    Medline Plus

    Full Text Available ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture Series All Training Information News & Events Press ...

  18. Clinical Epidemiology Unit - overview of research areas

    Science.gov (United States)

    Clinical Epidemiology Unit (CEU) conducts etiologic research with potential clinical and public health applications, and leads studies evaluating population-based early detection and cancer prevention strategies

  19. The acute effects of exercise on the microvascular volume of Achilles tendons in healthy young subjects

    DEFF Research Database (Denmark)

    Pingel, Jessica; Harrison, Adrian; Suetta, Charlotte

    2013-01-01

    Real-time harmonic contrast-enhanced ultrasound (CEU) is used in several diseases to visualize the microvascularization in various tissues, due to its high sensitivity.......Real-time harmonic contrast-enhanced ultrasound (CEU) is used in several diseases to visualize the microvascularization in various tissues, due to its high sensitivity....

  20. "Combining equity and utilitarianism"-additional insights into a novel approach

    NARCIS (Netherlands)

    Lemmen-Gerdessen, van Joke; Kanellopoulos, Argyris; Claassen, Frits

    2017-01-01

    Recently, a novel approach (to be referred to as CEU) was introduced for the frequently arising problem of combining the conflicting criteria of equity and utilitarianism. This paper provides additional insights into CEU and assesses its added value for practice by comparing it with a commonly

  1. Addendum: Pardo, J.; Zamora-Martínez, F.; Botella-Rocamora, P. Online Learning Algorithm for Time Series Forecasting Suitable for Low Cost Wireless Sensor Networks Nodes. Sensors 2015, 15, 9277–9304

    Directory of Open Access Journals (Sweden)

    Juan Pardo

    2015-07-01

    Full Text Available This work has been supported by the Consolidación de indicadores CEU-UCH2014-15 program of the Vicerrectorado de Investigación at Universidad CEU-Cardenal Herrera and financed by Generalitat Valenciana through Conselleria de Educación, Cultura y Deporte under project GV/2015/088. [...

  2. Kondenzirajući ostitis - prikaz slučaja

    OpenAIRE

    Dukić, Walter; Filipović-Zore, Irina; Bago, Ivona

    2006-01-01

    Kondenzirajući ostitis je patološko zadebljanje kosti u čeljustima, a karakteriziraju ga blagi klinički simptomi. Zadebljanje nastaje zbog poremećene pregradnje kosti, kao reakcija na blagu infekciju iz zubne pulpe. Ekstremno zadebljanje kosti lijeve strane maksile uzrokovalo je i asimetriju lica, a kliničkim te radiološkim pregledom nije se mogla postaviti konačna dijagnoza. Diferencijalno-dijagnostički, u obzir su dolazili svi tumori koštanog tkiva i cementom te kondenzirajući ostitis zb...

  3. Antibiotici u stomatološkoj praksi

    OpenAIRE

    Linčir, Ileana; Rošin-Grget, Kata

    1988-01-01

    Važan zadatak stomatologa je da u svom radu suzbija infekciju. Infekcije se iz usne šupijine mogu vrlo brzo raširiti i izazvati oboljenja koja katkada mogu biti i fatalna. U borbi protiv bakterijskih zaraza koriste se antibiotici. Stomatolog treba posegnuti za antibioticima u slučaju: 1) lije­čenja akutne orodentalne zaraze, 2) profilakse subakutnog bakterijskog endokarditisa ili zaštite bolesnika sa smanjenim obrambenim mehanizmi­ma i 3) u svrhu redukcije mikroorganizama u zubnom plaku, koji...

  4. Hospitālas urrīnceļu infekcijas profilakse un ārstēšana pie mazinvazīvas uroloģiskas iejaukšanās

    OpenAIRE

    Ņemirovska, Irina

    2015-01-01

    Urīnceļu infekcija pacientiem pēc ureteroskopiskas operācijas (URS) pie nierakmeņu slimības (NAS) ir nozīmīga medicīniska problēma. URS ir ļoti bieža un droša ambulatorā procedūra. Tomēr komplikācijas ir un tās neaprobežojas ar infekciju. Pētījuma mērķis un metodes: Zinātniskā pētījuma primārais mērķis bija iegūt datus par urīnceļu infekciju biežumu pēc mazinvazīvas uroloģiskas iejaukšanas (pēc NAS) Latvijā PSKUS Uroloģijas nodaļā un izanalizēt urīnceļu infekcijas epidemioloģiju, riska ...

  5. Tai Chi and Qi Gong for Health and Well-Being

    Medline Plus

    Full Text Available ... Development Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ...

  6. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... Development Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ...

  7. Full Spectrum Information Operations and the Information Professional Officer Intermediate Qualification Process: Filling the Gap to Ensure the Continued Leadership of the Information Professional Community in the Area of Information Dominance

    National Research Council Canada - National Science Library

    Velasco, Diego, Jr

    2005-01-01

    ...) Community to overhaul and improve the qualification process for its officers. The overall effort has included the addition of technical refresher courses, re-examination of the Continuing Education Units (CEU...

  8. Experiment list: ERX329652 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...organism=Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12891 || population=HapMap CEU || popu

  9. Experiment list: ERX329619 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...rganism=Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12892 || population=HapMap CEU || popul

  10. Experiment list: ERX329708 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12892 || population=HapMap CEU || population na

  11. Experiment list: ERX329682 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...rganism=Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12891 || population=HapMap CEU || popul

  12. Experiment list: ERX329651 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...m=Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12891 || population=HapMap CEU || population

  13. Experiment list: ERX329690 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...m=Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12892 || population=HapMap CEU || population

  14. Experiment list: ERX329632 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...=Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12892 || population=HapMap CEU || population n

  15. Experiment list: ERX329636 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12891 || population=HapMap CEU || population na

  16. Experiment list: ERX329661 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...ganism=Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12891 || population=HapMap CEU || popula

  17. Experiment list: ERX329612 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12892 || population=HapMap CEU || population na

  18. Experiment list: ERX329663 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12891 || population=HapMap CEU || population na

  19. Experiment list: ERX329639 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...organism=Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12892 || population=HapMap CEU || popu

  20. Experiment list: ERX329634 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...=Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12892 || population=HapMap CEU || population n

  1. Experiment list: ERX329719 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...rganism=Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12892 || population=HapMap CEU || popul

  2. Experiment list: ERX329611 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...organism=Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12892 || population=HapMap CEU || popu

  3. Experiment list: ERX329621 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...=Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12891 || population=HapMap CEU || population n

  4. Experiment list: ERX329714 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...organism=Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12891 || population=HapMap CEU || popu

  5. Experiment list: ERX329716 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstei...Homo sapiens || cell type=lymphoblastoid cell || cell line=NA12891 || population=HapMap CEU || population na

  6. [A pragmatic alliance. Jewisch-Lithuanian political cooperation at the beginning of the 20th century. Hrsg. von Vladas Sirutavičius, Darius Staliūnas] / Karsten Brüggemann

    Index Scriptorium Estoniae

    Brüggemann, Karsten, 1965-

    2013-01-01

    Arvustus: A pragmatic alliance. Jewisch-Lithuanian political cooperation at the beginning of the 20th century. Hrsg. von Vladas Sirutavičius und Darius Staliūnas. Verlag CEU Press. Budapest und New York, 2011

  7. Tai Chi and Qi Gong for Health and Well-Being

    Medline Plus

    Full Text Available ... For Health Care Professionals Clinical Practice Guidelines Literature Reviews All Health Information Research Research Results Results by ... Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU ...

  8. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... For Health Care Professionals Clinical Practice Guidelines Literature Reviews All Health Information Research Research Results Results by ... Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU ...

  9. Ultrasound Contrast Agents in the Study of Kidney Function in Health and Disease

    Science.gov (United States)

    Kalantarinia, Kambiz; Okusa, Mark D.

    2008-01-01

    Ultrasound contrast agents are gas filled microbubbles that enhance the ultrasound image. They behave similarly to red blood cells and cross all capillary beds; making contrast enhanced ultrasonography (CEU) a suitable technique to study vasculature and tissue blood flow. Ultrasound contrast agents have been found to be safe after intravenous injection. CEU has been used extensively in the field of cardiology. Currently, study of renal vasculature and renal blood flow requires complicated, time consuming and expensive techniques, which are not commonly used in clinical settings. CEU potentially may serve as a relatively noninvasive and safe technique for studying renal hemodynamics in health and disease. In this article we have reviewed the literature on the use of CEU in the study of kidney disease. PMID:19112526

  10. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series ...

  11. Tai Chi and Qi Gong for Health and Well-Being

    Medline Plus

    Full Text Available ... Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series ...

  12. Conference Experience for Undergraduates in the Division of Nuclear Physics - 10 Years Running

    Science.gov (United States)

    Rogers, Warren

    2008-04-01

    The Conference Experience for Undergraduates (CEU), held annually in the APS Division of Nuclear Physics since 1998, has become a valuable addition to the fall DNP meetings. Since its inception 10 years ago, approximately 730 undergraduate students from over 60 colleges and universities from around the country (and a few from abroad) have participated. The goal of the program is to provide students who have conducted undergraduate research in nuclear science a ``capstone'' conference experience, with the goal toward strengthening retention of talented students in the field. In addition to the main conference, the CEU includes extra activities for the students, including the main research poster session, two undergraduate nuclear physics seminars, and a graduate school information session. CEU application materials are considered by an independent review committee, and travel and lodging grants are awarded based on project merit. Financial support is provided by the NSF, DOE, and DNP. At the recent 10^th anniversary CEU, a mini-symposium was organized as part of the DNP conference, at which former CEU students (now graduate students, post-docs, and professors) had opportunity to talk about their research and the influence that undergraduate research and conference participation had on their career paths. Survey and anecdotal data indicating benefits of CEU participation, as well as initial results from career path tracking will be presented.

  13. Meticilīna rezistentais Staphylococcus epidermidis un tā izplatība veseliem cilvēkiem

    OpenAIRE

    Eriksone, Ilze

    2016-01-01

    Mūsdienās Staphylococcus epidermidis ir nozīmīgs oportūnistiskais patogēns. Tas ir viens no biežākajiem nozokomiālo infekciju izraisītājiem. Staphylococcus epidermidis celmu vidū plaši izplatīta ir rezistence pret antibakteriālajiem līdzekļiem. Maģistra darba mērķis bija izpētīt meticilīna rezistentā Staphylococcus epidermidis izplatību veseliem cilvēkiem. Meticilīna rezistentais Staphylococcus epidermidis netika konstatēts nevienā acs apakšējā plaksta konjunktīvā. Meticilīna rezistentais S...

  14. Meticilīna rezistentā Staphylococcus aureus ( MRSA) izraisītās suņu patoloģijas kā iespējamas MRSA infekcijas izplatības avots

    OpenAIRE

    Šuste, Rimute

    2014-01-01

    Darba mērķis bija noskaidrot, vai suņi spēj inficēties ar meticilīna rezistento Staphylococcus aureus (MRSA) un vai spēj šo infekciju pārnest cilvēkiem. Pētījumā tika izmantots materiāls no 256 suņiem ar ausu infekcijām Jelgavas novadā no 2011.gada novembra līdz 2012.gada augustam, kas tika analizētas, izmantojot dažādas mikrobioloģijas metodes MRSA celmu noteikšanai (BBL™ Crystal™ identifikācijas sistēma, Bauer-Kirby metode, “Slidex MRSA” atklāšanas tests u.c.). Saikne starp M...

  15. CENTROS EDUCACIONAIS UNIFICADOS DE SÃO PAULO: IMPLEMENTAÇÃO E CONTINUIDADE NUMA NOVA GESTÃO POLÍTICA

    OpenAIRE

    Cangussú, Lilian Cristina Pereira

    2011-01-01

    Este estudo tem como objetivo verificar se a política educacional do CEU: Centro Educacional Unificado criado e implementado no governo Municipal de Marta Suplicy também apresentado como uma proposta contra-hegemônica de escola inclusiva e cidadã, ainda existe nesta atual administração. Apresenta e identifica os princípios do projeto CEU, bem como suas principais diferenças dentro de duas perspectivas políticas: uma progressista e outra mais conservadora. Com a transição da administração p...

  16. Die „lex CEU“: der Tropfen, der das Fass zum Überlaufen bringt? Die ungarische Normsetzung an der Grenze des Rechtsstaates / Zoltán Rónay

    Index Scriptorium Estoniae

    Rónay, Zoltán

    2017-01-01

    Ungari kõrgharidusseaduse ja teiste seaduse muudatustest, mis puudutavad ka Kesk-Euroopa Ülikooli (seaduse hüüdnimi Lex CEU). Selle seaduse vastavusest õigusriigi põhimõtetele. Seaduse tekstid saksa keeles lk. 389-395

  17. Evaluation of HapMap data in six populations of European descent.

    NARCIS (Netherlands)

    Lundmark, P.E.; Liljedahl, U.; Boomsma, D.I.; Mannila, H.; Martin, N.G.; Palotie, A.; Peltonen, L.; Perola, M.; Spector, T.D.; Syvänen, A.C.

    2008-01-01

    We studied how well the European CEU samples used in the Haplotype Mapping Project (HapMap) represent five European populations by analyzing nuclear family samples from the Swedish, Finnish, Dutch, British and Australian (European ancestry) populations. The number of samples from each population

  18. Experiment list: ERX329709 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-B...apiens || cell type=lymphoblastoid cell || cell line=NA12878 || population=HapMap CEU || population name=CEP

  19. Experiment list: ERX329642 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-B...piens || cell type=lymphoblastoid cell || cell line=NA12878 || population=HapMap CEU || population name=CEPH

  20. Experiment list: ERX329638 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-B... cell type=lymphoblastoid cell || cell line=NA12878 || population=HapMap CEU || population name=CEPH || sex=

  1. Experiment list: ERX329644 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-B...| cell type=lymphoblastoid cell || cell line=NA12878 || population=HapMap CEU || population name=CEPH || sex

  2. Experiment list: ERX329687 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-B...|| cell type=lymphoblastoid cell || cell line=NA12878 || population=HapMap CEU || population name=CEPH || se

  3. Experiment list: ERX329712 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-B...apiens || cell type=lymphoblastoid cell || cell line=NA12878 || population=HapMap CEU || population name=CEP

  4. Experiment list: ERX329718 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-B... cell type=lymphoblastoid cell || cell line=NA12878 || population=HapMap CEU || population name=CEPH || sex=

  5. Experiment list: ERX329660 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-B...piens || cell type=lymphoblastoid cell || cell line=NA12878 || population=HapMap CEU || population name=CEPH

  6. Experiment list: ERX329707 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available ption=B-lymphocyte, lymphoblastoid, International HapMap Project - CEPH/Utah - European Caucasion, Epstein-B...| cell type=lymphoblastoid cell || cell line=NA12878 || population=HapMap CEU || population name=CEPH || sex

  7. Detection of Campylobacter species and Arcobacter butzleri in stool samples by use of real-time multiplex PCR

    NARCIS (Netherlands)

    R.F. de Boer (Richard); A. Ott (Alewijn); P. Güren (Pinar); E. van Zanten; A.F. van Belkum (Alex); A.M.D. Kooistra-Smid

    2013-01-01

    textabstractThe presence of Campylobacter (or Campylobacter-like) species in stools from patients suspected of infectious gastroenteritis (n = 493) was investigated using real-time PCR for detection of Arcobacter butzleri (hsp60 gene), Campylobacter coli (ceuE gene), Campylobacter jejuni (mapA),

  8. Tea Tree Oil

    Science.gov (United States)

    ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ... care provider. We encourage you to discuss any decisions about treatment or care with your health care provider. The mention of any product, service, or therapy is not an endorsement ... Dietary Supplements Wisely Herbs ...

  9. European Mistletoe

    Science.gov (United States)

    ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ... trees such as apple, oak, pine, and elm trees. Where the term “mistletoe” is used in this ... We encourage you to discuss any decisions about treatment or care with your health care ...

  10. European Elder (Elderberry)

    Science.gov (United States)

    ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ... berries, and other plant parts of the elder tree contain a toxic substance and, if not properly ... provider. We encourage you to discuss any decisions about treatment or care with your health care ...

  11. Chasteberry

    Science.gov (United States)

    ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ... 709. Mahady GB, Michel JL, Soni KK. Chaste Tree. In: Coates PM, Betz JM, Blackman MR, et al., ... We encourage you to discuss any decisions about treatment or care with your health care ...

  12. Horse Chestnut

    Science.gov (United States)

    ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ... and swelling after surgery. Preparations made from the tree’s bark are applied to ... We encourage you to discuss any decisions about treatment or care with your health care ...

  13. Yohimbe

    Science.gov (United States)

    ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ... dietary supplements made from the bark of the tree. How Much Do We Know? ... provider. We encourage you to discuss any decisions about treatment or care with your health care ...

  14. Lavender

    Science.gov (United States)

    ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ... et al. Possible efficacy of lavender and tea tree oils in the treatment of young women affected ... provider. We encourage you to discuss any decisions about treatment or care with your health care ...

  15. Cinnamon

    Science.gov (United States)

    ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ... cinnamon comes from the bark of the cinnamon tree. Essential oils are made from the bark, leaves, ... We encourage you to discuss any decisions about treatment or care with your health care ...

  16. A Business Case Study of Open Source Software

    Science.gov (United States)

    2001-07-01

    softlinux.com.br www.softlinux.com.br Investigacion y Desarrollo Agora www.id-agora.com SOT Finnish Software Engineering www.sot.com Iplabs www.iplabs.ru...College, Air University, June 11, 1999, http://ceu.fi.udc.es/GPUL/ articulos /varios/US_DoD_and_OSS.txt. 48. Slackware, www.slackware.com. 49. Slater, Derek

  17. Comparative analysis of the genomes of Shigella dysenteriae type 2 & type 7 isolates

    Directory of Open Access Journals (Sweden)

    Partha Pal

    2013-01-01

    Methods: Pulsed-field gel electrophoresis (PFGE technique was used to determine the diversity of Shigella genomes by rapid construction of physical maps. DNA end labelling, Southern hybridization and PCR techniques were also applied for mapping purposes. Results: The intron-coded enzyme I-CeuI cuts the bacterial genome specifically at its rrn operon. PFGE of I-CeuI digested S. dysenteriae genomes were found to carry seven rrn operons. However, I-CeuI profiles showed distinct restriction fragment polymorphism (RFLP between the isolates as well as with the whole genome sequenced isolates. Further studies revealed that the genome sizes and I-CeuI linkage maps of the S. dysenteriae type 7 and type 2 isolates were similar to that of S. dysenteriae type 1 and S. flexneri type 2a genomes, respectively. Interpretation & conclusions: Our findings indicate that the type 7 and type 1 isolates of S. dysenteriae were probably evolved from a same precursor, while the type 2 and S. flexneri type 2a were probably evolved and diversified from a common progenitor.

  18. Dense genotyping identifies and localizes multiple common and rare variant association signals in celiac disease

    NARCIS (Netherlands)

    Trynka, G.; Hunt, K.A.; Bockett, N.A.; Romanos, J.; Mistry, V.; Szperl, A.; Bakker, S.F.; Bardella, M.T.; Bhaw-Rosun, L.; Castillejo, G.; Concha, E. de la; Almeida, R.C. de; Dias, K.R.; Diemen, C.C. van; Dubois, P.C.; Duerr, R.H.; Edkins, S.; Franke, L.; Fransen, K.; Gutierrez, J.; Heap, G.A.; Hrdlickova, B.; Hunt, S.; Izurieta, L.P.; Izzo, V.; Joosten, L.A.B.; Langford, C.; Mazzilli, M.C.; Mein, C.A.; Midah, V.; Mitrovic, M.; Mora, B.; Morelli, M.; Nutland, S.; Nunez, C.; Onengut-Gumuscu, S.; Pearce, K.; Platteel, M.; Polanco, I.; Potter, S.; Ribes-Koninckx, C.; Ricano-Ponce, I.; Rich, S.S.; Rybak, A.; Santiago, J.L.; Senapati, S.; Sood, A.; Szajewska, H.; Troncone, R.; Varade, J.; Wallace, C.; Wolters, V.M.; Zhernakova, A.; Thelma, B.K.; Cukrowska, B.; Urcelay, E.; Bilbao, J.R.; Mearin, M.L.; Barisani, D.; Barrett, J.C.; Plagnol, V.; Deloukas, P.; Wijmenga, C.; Heel, D.A. van

    2011-01-01

    Using variants from the 1000 Genomes Project pilot European CEU dataset and data from additional resequencing studies, we densely genotyped 183 non-HLA risk loci previously associated with immune-mediated diseases in 12,041 individuals with celiac disease (cases) and 12,228 controls. We identified

  19. Hunner's Ulcers

    Science.gov (United States)

    ... instrument with a heated tip or a laser technique. Resection of ulcers : cutting around and removing the ulcers from ... Provider Registry Order Educational Materials CME/CEU IC Reading List Living with IC ... Sources of Fiber What to Eat The IC Plate™ Foods ...

  20. Randomized, Controlled Trial of CBT Training for PTSD Providers

    Science.gov (United States)

    2015-10-01

    o Securing CEU accreditation for the courses on web servers for psychology , social work, psychiatry , counseling , and nursing.  Web programming has...accreditation was secured previously for the four national professional organizations ( psychology , psychiatry , social work, and nursing) outlined in the...standardized patient, consultation INTRODUCTION Psychologically -based treatments and cognitive behavioral therapy (CBT) interventions have been shown to be

  1. Comparative analysis of the genomes of Shigella dysenteriae type 2 & type 7 isolates.

    Science.gov (United States)

    Pal, Partha; Pal, Arunima; Niyogi, Swapan Kumar; Ramamurthy, T; Bhadra, Rupak K

    2013-01-01

    The four species of the genus Shigella, namely, S. dysenteriae , S. flexneri, S. boydii and S. sonnei cause a wide spectrum of illness from watery diarrhoea to severe dysentery. Genomes of these four species show great diversity. In this study, NotI, XbaI or I-CeuI restriction enzyme digested genomes of two Shigella dysenteriae isolates belonging to the serotypes 2 and 7 were extensively analyzed to find their relatedness, if any, with the whole genome sequenced strains of S. dysenteriae type 1 and S. flexneri type 2a. Pulsed-field gel electrophoresis (PFGE) technique was used to determine the diversity of Shigella genomes by rapid construction of physical maps. DNA end labelling, Southern hybridization and PCR techniques were also applied for mapping purposes. The intron-coded enzyme I-CeuI cuts the bacterial genome specifically at its rrn operon. PFGE of I-CeuI digested S. dysenteriae genomes were found to carry seven rrn operons. However, I-CeuI profiles showed distinct restriction fragment polymorphism (RFLP) between the isolates as well as with the whole genome sequenced isolates. Further studies revealed that the genome sizes and I-CeuI linkage maps of the S. dysenteriae type 7 and type 2 isolates were similar to that of S. dysenteriae type 1 and S. flexneri type 2a genomes, respectively. Our findings indicate that the type 7 and type 1 isolates of S. dysenteriae were probably evolved from a same precursor, while the type 2 and S. flexneri type 2a were probably evolved and diversified from a common progenitor.

  2. Comparative analysis of the genomes of Shigella dysenteriae type 2 & type 7 isolates

    Science.gov (United States)

    Pal, Partha; Pal, Arunima; Niyogi, Swapan Kumar; Ramamurthy, T.; Bhadra, Rupak K.

    2013-01-01

    Background & objectives: The four species of the genus Shigella, namely, S. dysenteriae, S. flexneri, S. boydii and S. sonnei cause a wide spectrum of illness from watery diarrhoea to severe dysentery. Genomes of these four species show great diversity. In this study, NotI, XbaI or I-CeuI restriction enzyme digested genomes of two Shigella dysenteriae isolates belonging to the serotypes 2 and 7 were extensively analyzed to find their relatedness, if any, with the whole genome sequenced strains of S. dysenteriae type 1 and S. flexneri type 2a. Methods: Pulsed-field gel electrophoresis (PFGE) technique was used to determine the diversity of Shigella genomes by rapid construction of physical maps. DNA end labelling, Southern hybridization and PCR techniques were also applied for mapping purposes. Results: The intron-coded enzyme I-CeuI cuts the bacterial genome specifically at its rrn operon. PFGE of I-CeuI digested S. dysenteriae genomes were found to carry seven rrn operons. However, I-CeuI profiles showed distinct restriction fragment polymorphism (RFLP) between the isolates as well as with the whole genome sequenced isolates. Further studies revealed that the genome sizes and I-CeuI linkage maps of the S. dysenteriae type 7 and type 2 isolates were similar to that of S. dysenteriae type 1 and S. flexneri type 2a genomes, respectively. Interpretation & conclusions: Our findings indicate that the type 7 and type 1 isolates of S. dysenteriae were probably evolved from a same precursor, while the type 2 and S. flexneri type 2a were probably evolved and diversified from a common progenitor. PMID:23481068

  3. The microvascular volume of the achilles tendon is increased in patients with tendinopathy at rest and after a 1-hour treadmill run

    DEFF Research Database (Denmark)

    Pingel, Jessica; Harrison, Adrian; Simonsen, Lene

    2013-01-01

    BACKGROUND:Achilles tendinopathy (AT) is initiated asymptomatically and is therefore often discovered at a very late stage. PURPOSE:To elucidate whether the microvascular volume (MV) of the Achilles tendon is elevated in patients with AT compared with healthy controls during pre-exercise rest......, after acute exercise, and 24 hours after exercise. Additionally, this study investigated the muscle activation pattern of the gastrocnemius muscle and the relative elasticity of the Achilles tendon during a 1-hour treadmill run in healthy patients and in patients with AT. STUDY DESIGN......:Controlled laboratory study. METHODS:Real-time harmonic contrast-enhanced ultrasound (CEU) measurements of the MV of the Achilles tendon were taken in 18 volunteers (9 patients with AT, 9 healthy controls). The CEU analyses were conducted before exercise, immediately after a 1-hour treadmill run, and 24 hours after...

  4. Next-generation sequencing of human mitochondrial reference genomes uncovers high heteroplasmy frequency.

    Directory of Open Access Journals (Sweden)

    Maria Ximena Sosa

    Full Text Available We describe methods for rapid sequencing of the entire human mitochondrial genome (mtgenome, which involve long-range PCR for specific amplification of the mtgenome, pyrosequencing, quantitative mapping of sequence reads to identify sequence variants and heteroplasmy, as well as de novo sequence assembly. These methods have been used to study 40 publicly available HapMap samples of European (CEU and African (YRI ancestry to demonstrate a sequencing error rate <5.63×10(-4, nucleotide diversity of 1.6×10(-3 for CEU and 3.7×10(-3 for YRI, patterns of sequence variation consistent with earlier studies, but a higher rate of heteroplasmy varying between 10% and 50%. These results demonstrate that next-generation sequencing technologies allow interrogation of the mitochondrial genome in greater depth than previously possible which may be of value in biology and medicine.

  5. Detection of seven virulence and toxin genes of Campylobacter jejuni isolates from Danish turkeys by PCR and cytolethal distending toxin production of the isolates

    DEFF Research Database (Denmark)

    Bang, Dang Duong; Borck, Birgitte; Nielsen, Eva Møller

    2004-01-01

    A total of 117 Campylobacter jejuni isolates from Danish turkeys were tested for the presence of seven virulence and toxin genes by PCR. One hundred seventeen (100%) isolates were positive for flaA, cadF, and ceuE gene primers. One hundred three (88%) isolates were positive for cdt gene cluster P...... turkeys and calls for further investigation for the elimination of Campylobacter infection in industrial turkey production and in industrial food chains.......A total of 117 Campylobacter jejuni isolates from Danish turkeys were tested for the presence of seven virulence and toxin genes by PCR. One hundred seventeen (100%) isolates were positive for flaA, cadF, and ceuE gene primers. One hundred three (88%) isolates were positive for cdt gene cluster PCR...

  6. Geographical affinities of the HapMap samples.

    Directory of Open Access Journals (Sweden)

    Miao He

    Full Text Available BACKGROUND: The HapMap samples were collected for medical-genetic studies, but are also widely used in population-genetic and evolutionary investigations. Yet the ascertainment of the samples differs from most population-genetic studies which collect individuals who live in the same local region as their ancestors. What effects could this non-standard ascertainment have on the interpretation of HapMap results? METHODOLOGY/PRINCIPAL FINDINGS: We compared the HapMap samples with more conventionally-ascertained samples used in population- and forensic-genetic studies, including the HGDP-CEPH panel, making use of published genome-wide autosomal SNP data and Y-STR haplotypes, as well as producing new Y-STR data. We found that the HapMap samples were representative of their broad geographical regions of ancestry according to all tests applied. The YRI and JPT were indistinguishable from independent samples of Yoruba and Japanese in all ways investigated. However, both the CHB and the CEU were distinguishable from all other HGDP-CEPH populations with autosomal markers, and both showed Y-STR similarities to unusually large numbers of populations, perhaps reflecting their admixed origins. CONCLUSIONS/SIGNIFICANCE: The CHB and JPT are readily distinguished from one another with both autosomal and Y-chromosomal markers, and results obtained after combining them into a single sample should be interpreted with caution. The CEU are better described as being of Western European ancestry than of Northern European ancestry as often reported. Both the CHB and CEU show subtle but detectable signs of admixture. Thus the YRI and JPT samples are well-suited to standard population-genetic studies, but the CHB and CEU less so.

  7. Patient experiences in a critial care unit

    OpenAIRE

    2014-01-01

    M.Cur. (Intensive General Nursing Science) "Patient experiences in a critical contextual, qualitative research phenomenological method to obtain and objectives of the study are: care unit" is a study using the analyse data. The - to establish and describe how myocardial infarction patients experience the critical care unit (CeU) environment, and - to propose guidelines for optimal nursing care. The Nursing for the Whole Person Theory forms the paradigmatic framework of the study. The centr...

  8. Tag SNP selection for Finnish individuals based on the CEPH Utah HapMap database.

    Science.gov (United States)

    Willer, Cristen J; Scott, Laura J; Bonnycastle, Lori L; Jackson, Anne U; Chines, Peter; Pruim, Randall; Bark, Craig W; Tsai, Ya-Yu; Pugh, Elizabeth W; Doheny, Kimberly F; Kinnunen, Leena; Mohlke, Karen L; Valle, Timo T; Bergman, Richard N; Tuomilehto, Jaakko; Collins, Francis S; Boehnke, Michael

    2006-02-01

    The pattern and nature of linkage disequilibrium in the human genome is being studied and catalogued as part of the International HapMap Project [:2003 Nature 426:789-796]. A key goal of the HapMap Project is to enable identification of tag single nucleotide polymorphisms (SNPs) that capture a substantial portion of common human genetic variability while requiring only a small fraction of SNPs to be genotyped [International HapMap Consortium, 2005: Nature 437:1299-1320]. In the current study, we examined the effectiveness of using the CEU HapMap database to select tag SNPs for a Finnish sample. We selected SNPs in a 17.9-Mb region of chromosome 14 based on pairwise linkage disequilibrium (r(2)) estimates from the HapMap CEU sample, and genotyped 956 of these SNPs in 1,425 Finnish individuals. An excess of SNPs showed significantly different allele frequencies between the HapMap CEU and the Finnish samples, consistent with population-specific differences. However, we observed strong correlations between the two samples for estimates of allele frequencies, r(2) values, and haplotype frequencies. Our results demonstrate that the HapMap CEU samples provide an adequate basis for tag SNP selection in Finnish individuals, without the need to create a map specifically for the Finnish population, and suggest that the four-population HapMap data will provide useful information for tag SNP selection beyond the specific populations from which they were sampled. (c) 2005 Wiley-Liss, Inc.

  9. On the utility of data from the International HapMap Project for Australian association studies.

    Science.gov (United States)

    Stankovich, Jim; Cox, Charles J; Tan, Rachel B; Montgomery, Douglas S; Huxtable, Stewart J; Rubio, Justin P; Ehm, Margaret G; Johnson, Laura; Butzkueven, Helmut; Kilpatrick, Trevor J; Speed, Terence P; Roses, Allen D; Bahlo, Melanie; Foote, Simon J

    2006-03-01

    We compare patterns of linkage disequilibrium (LD) for 633 SNPs in two regions between samples collected in two Australian states and HapMap samples collected from Utah residents of Northern and Western (NW) European ancestry (CEU). Patterns of LD in the Australian and HapMap samples are similar, and tag SNPs chosen using HapMap genotypes perform almost as well on Australian samples as tags chosen using Australian genotypes.

  10. Inferential genotyping of Y chromosomes in Latter-Day Saints founders and comparison to Utah samples in the HapMap project.

    Science.gov (United States)

    Gitschier, Jane

    2009-02-01

    One concern in human genetics research is maintaining the privacy of study participants. The growth in genealogical registries may contribute to loss of privacy, given that genotypic information is accessible online to facilitate discovery of genetic relationships. Through iterative use of two such web archives, FamilySearch and Sorenson Molecular Genealogy Foundation, I was able to discern the likely haplotypes for the Y chromosomes of two men, Joseph Smith and Brigham Young, who were instrumental in the founding of the Latter-Day Saints Church. I then determined whether any of the Utahns who contributed to the HapMap project (the "CEU" set) is related to either man, on the basis of haplotype analysis of the Y chromosome. Although none of the CEU contributors appear to be a male-line relative, I discovered that predictions could be made for the surnames of the CEU participants by a similar process. For 20 of the 30 unrelated CEU samples, at least one exact match was revealed, and for 17 of these, a potential ancestor from Utah or a neighboring state could be identified. For the remaining ten samples, a match was nearly perfect, typically deviating by only one marker repeat unit. The same query performed in two other large databases revealed fewer individual matches and helped to clarify which surname predictions are more likely to be correct. Because large data sets of genotypes from both consenting research subjects and individuals pursuing genetic genealogy will be accessible online, this type of triangulation between databases may compromise the privacy of research subjects.

  11. Cervical cancer screening in adolescents: an evidence-based internet education program for practice improvement among advanced practice nurses.

    Science.gov (United States)

    Choma, Kim; McKeever, Amy E

    2015-02-01

    The literature reports great variation in the knowledge levels and application of the recent changes of cervical cancer screening guidelines into clinical practice. Evidence-based screening guidelines for the prevention and early detection of cervical cancer offers healthcare providers the opportunity to improve practice patterns among female adolescents by decreasing psychological distress as well as reducing healthcare costs and morbidities associated with over-screening. The purpose of this pilot intervention study was to determine the effects of a Web-based continuing education unit (CEU) program on advanced practice nurses' (APNs) knowledge of current cervical cancer screening evidence-based recommendations and their application in practice. This paper presents a process improvement project as an example of a way to disseminate updated evidence-based practice guidelines among busy healthcare providers. This Web-based CEU program was developed, piloted, and evaluated specifically for APNs. The program addressed their knowledge level of cervical cancer and its relationship with high-risk human papillomavirus. It also addressed the new cervical cancer screening guidelines and the application of those guidelines into clinical practice. Results of the study indicated that knowledge gaps exist among APNs about cervical cancer screening in adolescents. However, when provided with a CEU educational intervention, APNs' knowledge levels increased and their self-reported clinical practice behaviors changed in accordance with the new cervical cancer screening guidelines. Providing convenient and readily accessible up-to-date electronic content that provides CEU enhances the adoption of clinical practice guidelines, thereby decreasing the potential of the morbidities associated with over-screening for cervical cancer in adolescents and young women. © 2014 Sigma Theta Tau International.

  12. Genetic Variations in Mitochondria and Prostate Cancer Aggressiveness and Progression in Caucasian and African American Men

    Science.gov (United States)

    2015-09-01

    ancestry . Interestingly, mtDNA copy number was found positively associated with YRI ancestry (ρ=0.103, Pɘ.001) and inversely associated with CEU... ancestry (ρ=0.104, Pɘ.001). Next, we examined relationships between mtDNA copy number levels and selected epidemiologic variables (Table 2), stratified...associated with mtDNA copy number levels in 94 healthy young participants (Pɘ.01) [69]. In 144 postmenopausal women , Kim et al found that the

  13. Representation of women as editors in the Cochrane collaboration.

    Science.gov (United States)

    Bhaumik, Soumyadeep; Mathew, Rebecca Joyce

    2014-12-01

    There is considerable gender disparity in editorial boards of medical journals. Being an editor in a Cochrane review group (CRG), like being an editor in a medical journal, is an indirect representation of one's reputation and leadership abilities in a particular specialty. The aim of the study was to analyze the representation of women editors on the editorial teams of CRG's and the Central Editorial Unit (CEU) of the Cochrane Collaboration. Information about editorial team members of CRGs and the CEU was extracted from respective websites. Gender of the individual was determined by inspection of names, individual profile description or photographs in the CRG or institutional webpage, social networking sites and internet search. Data was validated by two authors independently and differences sorted by consensus. A total of 788 editors across all CRGs and the CEU with an overall 371 females (47.1%) and 417 (52.9%) males were identified. of the CEU editors, 62.5% were females. There were 68 coordinating editors (35.3% females), and 62 managing editors (56% females), who provided leadership to the CRGs. Eighty-four percent of trial search coordinators were found to be females. Ten CRGs had 75% or more of its editors as females while 7 CRGs had less than or equal to 25% female editors. The representation of women editors in the Cochrane Collaboration was found to be better than in editorial boards of medical journals. There is still scope for improvement to ensure better gender diversity across all roles and in all CRG's. © 2014 Chinese Cochrane Center, West China Hospital of Sichuan University and Wiley Publishing Asia Pty Ltd.

  14. SOME OBSERVATIONS ON TIIE EFFECT OF GRASSSEED ...

    African Journals Online (AJOL)

    Approxirmtcly 509jof RhodG* b ovcrod by rdd typcr of which HAaoWgon @rrtatlrr cod@ r +nfficllr proportion of tb ga.i & If shcep other than thc tnrooth qtod indlanour typcf lrt introdu.td h thcr ercra fu F& of tt dtAq ftorr3lr hySrorcoph actlon, penctrltt the *in and, lf prclcct in rny nun$cr, rny rcndcr thc cucrrs urttrrc3iw rd ceu* fcfifu.

  15. Discrimination between Native and Tn6010-Associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by Using a Two-Step Strategy

    DEFF Research Database (Denmark)

    Guillard, Thomas; Lebreil, Anne-Laure; Hansen, Lars Hestbjerg

    2015-01-01

    ABSTRACT We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find that ...... that Klebsiella sp. and Raoultella sp. reference strains chromosomally carried oqxAB....

  16. Burnup simulations of different fuel grades using the MCNPX Monte Carlo code

    Directory of Open Access Journals (Sweden)

    Asah-Opoku Fiifi

    2014-01-01

    Full Text Available Global energy problems range from the increasing cost of fuel to the unequal distribution of energy resources and the potential climate change resulting from the burning of fossil fuels. A sustainable nuclear energy would augment the current world energy supply and serve as a reliable future energy source. This research focuses on Monte Carlo simulations of pressurized water reactor systems. Three different fuel grades - mixed oxide fuel (MOX, uranium oxide fuel (UOX, and commercially enriched uranium or uranium metal (CEU - are used in this simulation and their impact on the effective multiplication factor (Keff and, hence, criticality and total radioactivity of the reactor core after fuel burnup analyzed. The effect of different clad materials on Keff is also studied. Burnup calculation results indicate a buildup of plutonium isotopes in UOX and CEU, as opposed to a decline in plutonium radioisotopes for MOX fuel burnup time. For MOX fuel, a decrease of 31.9% of the fissile plutonium isotope is observed, while for UOX and CEU, fissile plutonium isotopes increased by 82.3% and 83.8%, respectively. Keff results show zircaloy as a much more effective clad material in comparison to zirconium and stainless steel.

  17. Cell-Edge-Aware Precoding for Downlink Massive MIMO Cellular Networks

    Science.gov (United States)

    Yang, Howard H.; Geraci, Giovanni; Quek, Tony Q. S.; Andrews, Jeffrey G.

    2017-07-01

    We propose a cell-edge-aware (CEA) zero forcing (ZF) precoder that exploits the excess spatial degrees of freedom provided by a large number of base station (BS) antennas to suppress inter-cell interference at the most vulnerable user equipments (UEs). We evaluate the downlink performance of CEA-ZF, as well as that of a conventional cell-edge-unaware (CEU) ZF precoder in a network with random base station topology. Our analysis and simulations show that the proposed CEA-ZF precoder outperforms CEU-ZF precoding in terms of (i) aggregate per-cell data rate, (ii) coverage probability, and (iii) 95%-likely, or edge user, rate. In particular, when both perfect channel state information and a large number of antennas N are available at the BSs, we demonstrate that the outage probability under CEA-ZF and CEU-ZF decay as 1/N^2 and 1/N, respectively. This result identifies CEA-ZF as a more effective precoding scheme for massive MIMO cellular networks. Our framework also reveals the importance of scheduling the optimal number of UEs per BS, and confirms the necessity to control the amount of pilot contamination received during the channel estimation phase.

  18. Population differences in transcript-regulator expression quantitative trait loci.

    Directory of Open Access Journals (Sweden)

    Pierre R Bushel

    Full Text Available Gene expression quantitative trait loci (eQTL are useful for identifying single nucleotide polymorphisms (SNPs associated with diseases. At times, a genetic variant may be associated with a master regulator involved in the manifestation of a disease. The downstream target genes of the master regulator are typically co-expressed and share biological function. Therefore, it is practical to screen for eQTLs by identifying SNPs associated with the targets of a transcript-regulator (TR. We used a multivariate regression with the gene expression of known targets of TRs and SNPs to identify TReQTLs in European (CEU and African (YRI HapMap populations. A nominal p-value of <1×10(-6 revealed 234 SNPs in CEU and 154 in YRI as TReQTLs. These represent 36 independent (tag SNPs in CEU and 39 in YRI affecting the downstream targets of 25 and 36 TRs respectively. At a false discovery rate (FDR = 45%, one cis-acting tag SNP (within 1 kb of a gene in each population was identified as a TReQTL. In CEU, the SNP (rs16858621 in Pcnxl2 was found to be associated with the genes regulated by CREM whereas in YRI, the SNP (rs16909324 was linked to the targets of miRNA hsa-miR-125a. To infer the pathways that regulate expression, we ranked TReQTLs by connectivity within the structure of biological process subtrees. One TReQTL SNP (rs3790904 in CEU maps to Lphn2 and is associated (nominal p-value = 8.1×10(-7 with the targets of the X-linked breast cancer suppressor Foxp3. The structure of the biological process subtree and a gene interaction network of the TReQTL revealed that tumor necrosis factor, NF-kappaB and variants in G-protein coupled receptors signaling may play a central role as communicators in Foxp3 functional regulation. The potential pleiotropic effect of the Foxp3 TReQTLs was gleaned from integrating mRNA-Seq data and SNP-set enrichment into the analysis.

  19. An evaluation of the performance of tag SNPs derived from HapMap in a Caucasian population.

    Directory of Open Access Journals (Sweden)

    Alexandre Montpetit

    2006-03-01

    Full Text Available The Haplotype Map (HapMap project recently generated genotype data for more than 1 million single-nucleotide polymorphisms (SNPs in four population samples. The main application of the data is in the selection of tag single-nucleotide polymorphisms (tSNPs to use in association studies. The usefulness of this selection process needs to be verified in populations outside those used for the HapMap project. In addition, it is not known how well the data represent the general population, as only 90-120 chromosomes were used for each population and since the genotyped SNPs were selected so as to have high frequencies. In this study, we analyzed more than 1,000 individuals from Estonia. The population of this northern European country has been influenced by many different waves of migrations from Europe and Russia. We genotyped 1,536 randomly selected SNPs from two 500-kbp ENCODE regions on Chromosome 2. We observed that the tSNPs selected from the CEPH (Centre d'Etude du Polymorphisme Humain from Utah (CEU HapMap samples (derived from US residents with northern and western European ancestry captured most of the variation in the Estonia sample. (Between 90% and 95% of the SNPs with a minor allele frequency of more than 5% have an r2 of at least 0.8 with one of the CEU tSNPs. Using the reverse approach, tags selected from the Estonia sample could almost equally well describe the CEU sample. Finally, we observed that the sample size, the allelic frequency, and the SNP density in the dataset used to select the tags each have important effects on the tagging performance. Overall, our study supports the use of HapMap data in other Caucasian populations, but the SNP density and the bias towards high-frequency SNPs have to be taken into account when designing association studies.

  20. An evaluation of the performance of tag SNPs derived from HapMap in a Caucasian population.

    Science.gov (United States)

    Montpetit, Alexandre; Nelis, Mari; Laflamme, Philippe; Magi, Reedik; Ke, Xiayi; Remm, Maido; Cardon, Lon; Hudson, Thomas J; Metspalu, Andres

    2006-03-01

    The Haplotype Map (HapMap) project recently generated genotype data for more than 1 million single-nucleotide polymorphisms (SNPs) in four population samples. The main application of the data is in the selection of tag single-nucleotide polymorphisms (tSNPs) to use in association studies. The usefulness of this selection process needs to be verified in populations outside those used for the HapMap project. In addition, it is not known how well the data represent the general population, as only 90-120 chromosomes were used for each population and since the genotyped SNPs were selected so as to have high frequencies. In this study, we analyzed more than 1,000 individuals from Estonia. The population of this northern European country has been influenced by many different waves of migrations from Europe and Russia. We genotyped 1,536 randomly selected SNPs from two 500-kbp ENCODE regions on Chromosome 2. We observed that the tSNPs selected from the CEPH (Centre d'Etude du Polymorphisme Humain) from Utah (CEU) HapMap samples (derived from US residents with northern and western European ancestry) captured most of the variation in the Estonia sample. (Between 90% and 95% of the SNPs with a minor allele frequency of more than 5% have an r2 of at least 0.8 with one of the CEU tSNPs.) Using the reverse approach, tags selected from the Estonia sample could almost equally well describe the CEU sample. Finally, we observed that the sample size, the allelic frequency, and the SNP density in the dataset used to select the tags each have important effects on the tagging performance. Overall, our study supports the use of HapMap data in other Caucasian populations, but the SNP density and the bias towards high-frequency SNPs have to be taken into account when designing association studies.

  1. Engaging undergraduate students in hadron physics research and instrumentation

    Science.gov (United States)

    Horn, Tanja

    2017-09-01

    Nuclear physics research is fundamental to our understanding of the visible universe and at the same time intertwined with our daily life. Nuclear physics studies the origin and structure of the atomic nuclei in terms of their basic constituents, the quarks and gluons. Atoms and molecules would not exist without underlying quark-gluon interactions, which build nearly all the mass of the visible universe from an assembly of massless gluons and nearly-massless quarks. The study of hadron structure with electromagnetic probes through exclusive and semi-inclusive scattering experiments carried out at the 12 GeV Jefferson Laboratory plays an important role in this effort. In particular, planned precision measurements of pion and kaon form factors and longitudinal-transverse separated deep exclusive pion and kaon electroproduction cross sections to the highest momentum transfers achievable play an important role in understanding hadron structure and masses and provide essential constraints for 3D hadron imaging. While a growing fraction of nuclear physics research is carried out at large international laboratories, individual university research groups play critical roles in the success of that research. These include data analysis projects and the development of state-of-the-art instrumentation demanded by increasingly sophisticated experiments. These efforts are empowered by the creativity of university faculty, staff, postdocs, and provide students with unique hands-on experience. As an example, an aerogel Cherenkov detector enabling strangeness physics research in Hall C at Jefferson Lab was constructed at the Catholic University of America with the help of 16 undergraduate and high school students. The ''Conference Experience for Undergraduates'' (CEU) provides a venue for these students who have conducted research in nuclear physics. This presentation will present the experiences of one of the participants in the first years of the CEU, her current research program

  2. The CFTR Met 470 allele is associated with lower birth rates in fertile men from a population isolate.

    Directory of Open Access Journals (Sweden)

    Gülüm Kosova

    2010-06-01

    Full Text Available Although little is known about the role of the cystic fibrosis transmembrane regulator (CFTR gene in reproductive physiology, numerous variants in this gene have been implicated in etiology of male infertility due to congenital bilateral absence of the vas deferens (CBAVD. Here, we studied the fertility effects of three CBAVD-associated CFTR polymorphisms, the (TGm and polyT repeat polymorphisms in intron 8 and Met470Val in exon 10, in healthy men of European descent. Homozygosity for the Met470 allele was associated with lower birth rates, defined as the number of births per year of marriage (P = 0.0029. The Met470Val locus explained 4.36% of the phenotypic variance in birth rate, and men homozygous for the Met470 allele had 0.56 fewer children on average compared to Val470 carrier men. The derived Val470 allele occurs at high frequencies in non-African populations (allele frequency = 0.51 in HapMap CEU, whereas it is very rare in African population (Fst = 0.43 between HapMap CEU and YRI. In addition, haplotypes bearing Val470 show a lack of genetic diversity and are thus longer than haplotypes bearing Met470 (measured by an integrated haplotype score [iHS] of -1.93 in HapMap CEU. The fraction of SNPs in the HapMap Phase2 data set with more extreme Fst and iHS measures is 0.003, consistent with a selective sweep outside of Africa. The fertility advantage conferred by Val470 relative to Met470 may provide a selective mechanism for these population genetic observations.

  3. Inferring the joint demographic history of multiple populations from multidimensional SNP frequency data.

    Directory of Open Access Journals (Sweden)

    Ryan N Gutenkunst

    2009-10-01

    Full Text Available Demographic models built from genetic data play important roles in illuminating prehistorical events and serving as null models in genome scans for selection. We introduce an inference method based on the joint frequency spectrum of genetic variants within and between populations. For candidate models we numerically compute the expected spectrum using a diffusion approximation to the one-locus, two-allele Wright-Fisher process, involving up to three simultaneous populations. Our approach is a composite likelihood scheme, since linkage between neutral loci alters the variance but not the expectation of the frequency spectrum. We thus use bootstraps incorporating linkage to estimate uncertainties for parameters and significance values for hypothesis tests. Our method can also incorporate selection on single sites, predicting the joint distribution of selected alleles among populations experiencing a bevy of evolutionary forces, including expansions, contractions, migrations, and admixture. We model human expansion out of Africa and the settlement of the New World, using 5 Mb of noncoding DNA resequenced in 68 individuals from 4 populations (YRI, CHB, CEU, and MXL by the Environmental Genome Project. We infer divergence between West African and Eurasian populations 140 thousand years ago (95% confidence interval: 40-270 kya. This is earlier than other genetic studies, in part because we incorporate migration. We estimate the European (CEU and East Asian (CHB divergence time to be 23 kya (95% c.i.: 17-43 kya, long after archeological evidence places modern humans in Europe. Finally, we estimate divergence between East Asians (CHB and Mexican-Americans (MXL of 22 kya (95% c.i.: 16.3-26.9 kya, and our analysis yields no evidence for subsequent migration. Furthermore, combining our demographic model with a previously estimated distribution of selective effects among newly arising amino acid mutations accurately predicts the frequency spectrum of

  4. Pornographie et sexualité des adolescents: la consommation de pornographie influence-t-elle la perception de la sexualité des adolescents?

    OpenAIRE

    Briguet, Marie-France; Spieldenner, Jörg

    2007-01-01

    Actuellement, les multiples moyens de diffusion de la pornographie la rendent facilement accessible aux adolescents. Dans cette recherche, 97% des adolescents interrogés ont vu de la pornographie, la plupart avant 16 ans, l’âge légal de vente de matériel pornographique. Certains en consomment régulièrement, en particulier ceux qui en ont vu en étant jeunes. Certains considèrent que la pornographie peut leur apprendre à faire l’amour ou est représentative de la réalité, en particulier ceu...

  5. Validation and Interrogation of Differentially Expressed and Alternatively Spliced Genes in African American Prostate Cancer

    Science.gov (United States)

    2016-10-01

    HapMap 3 Project . (A) rs116458171 and MET mRNA expression level in 326 Africans, and (B) rs2072454 and EGFR mRNA expression level in 107 Europeans...lymphoblastoid cell lines of 716 individuals from HapMap 3 Project including 107 CEU, 242 CHB, 41 MEX and 326 AFR populations. (A) rs35605 and ABCC1 (reporter...Prostate Cancer 5a. CONTRACT NUMBER 5b. GRANT NUMBER W81XWH-14-1-0569 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER Steven Patierno, PhD

  6. The regulatory effect of miRNAs is a heritable genetic trait in humans

    Science.gov (United States)

    2012-01-01

    Background microRNAs (miRNAs) have been shown to regulate the expression of a large number of genes and play key roles in many biological processes. Several previous studies have quantified the inhibitory effect of a miRNA indirectly by considering the expression levels of genes that are predicted to be targeted by the miRNA and this approach has been shown to be robust to the choice of prediction algorithm. Given a gene expression dataset, Cheng et al. defined the regulatory effect score (RE-score) of a miRNA as the difference in the gene expression rank of targets of the miRNA compared to non-targeted genes. Results Using microarray data from parent-offspring trios from the International HapMap project, we show that the RE-score of most miRNAs is correlated between parents and offspring and, thus, inter-individual variation in RE-score has a genetic component in humans. Indeed, the mean RE-score across miRNAs is correlated between parents and offspring, suggesting genetic differences in the overall efficiency of the miRNA biogenesis pathway between individuals. To explore the genetics of this quantitative trait further, we carried out a genome-wide association study of the mean RE-score separately in two HapMap populations (CEU and YRI). No genome-wide significant associations were discovered; however, a SNP rs17409624, in an intron of DROSHA, was significantly associated with mean RE-score in the CEU population following permutation-based control for multiple testing based on all SNPs mapped to the canonical miRNA biogenesis pathway; of 244 individual miRNA RE-scores assessed in the CEU, 214 were associated (p < 0.05) with rs17409624. The SNP was also nominally significantly associated (p = 0.04) with mean RE-score in the YRI population. Interestingly, the same SNP was associated with 17 (8.5% of all expressed) miRNA expression levels in the CEU. We also show here that the expression of the targets of most miRNAs is more highly correlated with global changes in

  7. Genetic variants in cytosolic 5'-nucleotidase II are associated with its expression and cytarabine sensitivity in HapMap cell lines and in patients with acute myeloid leukemia.

    Science.gov (United States)

    Mitra, Amit K; Crews, Kristine R; Pounds, Stanley; Cao, Xueyuan; Feldberg, Tanya; Ghodke, Yogita; Gandhi, Varsha; Plunkett, William; Dolan, M Eileen; Hartford, Christine; Raimondi, Susana; Campana, Dario; Downing, James; Rubnitz, Jeffrey E; Ribeiro, Raul C; Lamba, Jatinder K

    2011-10-01

    Cytosolic 5'-nucleotidase II (NT5C2) is involved in the development of 1-β-d-arabinofuranosylcytosine (ara-C) resistance and has been associated with clinical outcome in patients receiving ara-C-based chemotherapy. NT5C2 inactivates ara-C by dephosphorylating ara-C monophosphate to ara-C. In this study, we sequenced NT5C2 in genomic DNA samples from International HapMap project panels with European [Centre d'Etude du Polymorphisme Humain (CEU); n = 90] or African [Yoruba people in Ibadan, Nigeria (YRI); n = 90] ancestry. We identified 41 genetic variants [one insertion-deletion and 40 single nucleotide polymorphisms (SNPs)], including three nonsynonymous SNPs (Y3A, K47R, and Q136R). Twenty-five SNPs were novel and 16 overlapped with the HapMap data. Subjects with African ancestry had NT5C2 mRNA expression levels that was significantly higher than those with European ancestry (p = 0.005). Furthermore, there was a correlation between NT5C2 mRNA expression and ara-C sensitivity in CEU but not in YRI cell lines. None of the nonsynonymous SNPs demonstrated any effect on NT5C2 activity. The genotypes of several SNPs were significantly associated with NT5C2 mRNA expression and/or ara-C sensitivity in CEU cell lines, but very few were significant in YRI cell lines. Of most interest, SNPs (linkage disequilibrium group CEU.12) in the 5'-untranslated region were associated with NT5C2 expression and ara-C sensitivity in HapMap cell lines and with NT5C2 mRNA expression and ara-C sensitivity in diagnostic leukemic blasts from pediatric patients with acute myeloid leukemia. Functional genomics analysis demonstrated that the promoter SNP rs11191612 was associated with altered luciferase activation in reporter assays and altered DNA-protein binding in gel shift assays. These results suggest that genetic variations in NT5C2 influence its expression and, potentially, cellular responses to nucleoside analogs.

  8. Genotype imputation for African Americans using data from HapMap phase II versus 1000 genomes projects.

    Science.gov (United States)

    Sung, Yun J; Gu, C Charles; Tiwari, Hemant K; Arnett, Donna K; Broeckel, Ulrich; Rao, Dabeeru C

    2012-07-01

    Genotype imputation provides imputation of untyped single nucleotide polymorphisms (SNPs) that are present on a reference panel such as those from the HapMap Project. It is popular for increasing statistical power and comparing results across studies using different platforms. Imputation for African American populations is challenging because their linkage disequilibrium blocks are shorter and also because no ideal reference panel is available due to admixture. In this paper, we evaluated three imputation strategies for African Americans. The intersection strategy used a combined panel consisting of SNPs polymorphic in both CEU and YRI. The union strategy used a panel consisting of SNPs polymorphic in either CEU or YRI. The merge strategy merged results from two separate imputations, one using CEU and the other using YRI. Because recent investigators are increasingly using the data from the 1000 Genomes (1KG) Project for genotype imputation, we evaluated both 1KG-based imputations and HapMap-based imputations. We used 23,707 SNPs from chromosomes 21 and 22 on Affymetrix SNP Array 6.0 genotyped for 1,075 HyperGEN African Americans. We found that 1KG-based imputations provided a substantially larger number of variants than HapMap-based imputations, about three times as many common variants and eight times as many rare and low-frequency variants. This higher yield is expected because the 1KG panel includes more SNPs. Accuracy rates using 1KG data were slightly lower than those using HapMap data before filtering, but slightly higher after filtering. The union strategy provided the highest imputation yield with next highest accuracy. The intersection strategy provided the lowest imputation yield but the highest accuracy. The merge strategy provided the lowest imputation accuracy. We observed that SNPs polymorphic only in CEU had much lower accuracy, reducing the accuracy of the union strategy. Our findings suggest that 1KG-based imputations can facilitate discovery of

  9. Identification of Chicken Originated Campylobacter coli and Campylobacter jejuni by Polymerase Chain Reaction (PCR)

    OpenAIRE

    ERTAŞ, Hasan Basri; ÇETİNKAYA, Burhan; MUZ, Adile; ÖNGÖR, Hasan

    2014-01-01

    The purpose of this study was to isolate Campylobacter species from the intestines and livers of chicken and to identify Campylobacter coli and Campylobacter jejuni by both conventional methods and Polymerase Chain Reaction (PCR). Four specific primers derived from the ceuE gene present in the genomes of C. coli and C. jejuni were used for PCR identification. In the examination of 150 intestine and liver samples by culture and PCR, 25 (16.6%) and 32 (21.3%) were identified as C. coli and C...

  10. Actitud de los alumnos de quinto de medicina hacia la salud mental Medicine 5th course student's attitudes towards mental health

    OpenAIRE

    Inmaculada Gilaberte; Inmaculada Failde; Alejandro Salazar; Luis Caballero

    2012-01-01

    Introducción. El estigma social que persiste hacia la enfermedad mental puede estar presente en los estudiantes de medicina e impactar en la forma de considerar ésta y la psiquiatría como especialidad. Objetivo. Investigar la actitud hacia la enfermedad mental y la psiquiatría en una muestra de estudiantes. Sujetos y métodos. Se realizó una encuesta estructurada, voluntaria y anónima a los 27 estudiantes de quinto curso del grado de medicina de la Universidad San Pablo-CEU de Madrid. La encue...

  11. Actitud de los alumnos de 5º de medicina hacia la Salud Mental.

    OpenAIRE

    Gilaberte, Inmaculada; Failde, Inmaculada; Salazar, Alejandro; Caballero, Luis

    2012-01-01

    Introducción. El estigma social que persiste hacia la enfermedad mental puede estar presente en los estudiantes de medicina e impactar en la forma de considerar ésta y la psiquiatría como especialidad. Objetivo. Investigar la actitud hacia la enfermedad mental y la psiquiatría en una muestra de estudiantes. Sujetos y métodos. Se realizó una encuesta estructurada, voluntaria y anónima a los 27 estudiantes de quinto curso del grado de medicina de la Universidad San Pablo-CEU de Madr...

  12. Universos y categorías de análisis del envejecimiento activo en los magacines de radio en España

    OpenAIRE

    Suay Madrid, Amparo

    2016-01-01

    Esta clasificación la hemos creado en la Universidad CEU Cardenal Herrera de Alfara del Patriarca en Valencia, a partir de una investigación centrada en el discurso del envejecimiento activo, en cuanto a calidad de vida, participación social e incremento de la ciudadanía. Para ello, hemos realizado un análisis empírico en los magacines de la radio española desde febrero de 2014 a enero de 2015, contemplando para ello dos muestras de programas. En estas muestras de program...

  13. Um estudo sobre os sentidos e significados de técnicos de Educação Física de um Centro Educacional Unificado sobre a competição esportiva escolar

    OpenAIRE

    Fontes, Vinícius Costa

    2013-01-01

    Este trabalho tem como objetivo analisar as significações constituídas pelos Técnicos de Educação Física de um Centro Educacional Unificado (CEU), sobre o esporte competitivo escolar. A competição desportiva escolar é considerada, tanto pelos entrevistados quanto pela literatura, um recurso potencialmente didático, sendo necessário compreender a utilização e o direcionamento das políticas públicas e dos técnicos deste recurso, considerando os mediadores socioculturais da escola, do esporte, d...

  14. The regulatory effect of miRNAs is a heritable genetic trait in humans

    Directory of Open Access Journals (Sweden)

    Geeleher Paul

    2012-08-01

    Full Text Available Abstract Background microRNAs (miRNAs have been shown to regulate the expression of a large number of genes and play key roles in many biological processes. Several previous studies have quantified the inhibitory effect of a miRNA indirectly by considering the expression levels of genes that are predicted to be targeted by the miRNA and this approach has been shown to be robust to the choice of prediction algorithm. Given a gene expression dataset, Cheng et al. defined the regulatory effect score (RE-score of a miRNA as the difference in the gene expression rank of targets of the miRNA compared to non-targeted genes. Results Using microarray data from parent-offspring trios from the International HapMap project, we show that the RE-score of most miRNAs is correlated between parents and offspring and, thus, inter-individual variation in RE-score has a genetic component in humans. Indeed, the mean RE-score across miRNAs is correlated between parents and offspring, suggesting genetic differences in the overall efficiency of the miRNA biogenesis pathway between individuals. To explore the genetics of this quantitative trait further, we carried out a genome-wide association study of the mean RE-score separately in two HapMap populations (CEU and YRI. No genome-wide significant associations were discovered; however, a SNP rs17409624, in an intron of DROSHA, was significantly associated with mean RE-score in the CEU population following permutation-based control for multiple testing based on all SNPs mapped to the canonical miRNA biogenesis pathway; of 244 individual miRNA RE-scores assessed in the CEU, 214 were associated (p p = 0.04 with mean RE-score in the YRI population. Interestingly, the same SNP was associated with 17 (8.5% of all expressed miRNA expression levels in the CEU. We also show here that the expression of the targets of most miRNAs is more highly correlated with global changes in miRNA regulatory effect than with the expression of

  15. Chemotherapy Necessitates Increased Immune Control of HHVs: A Cause of Persistent Inflammation Enabling Protracted Fatigue in Breast Cancer Survivors

    Science.gov (United States)

    2015-06-01

    We hypothesized that chemotherapy would iuduce a shift iu control of chronic luunan he1pes viruses (HHVs) iu breast cancer patients and that this...previously banked frozen PBMC and data from frozen samples proved unreliable given a lack of positive cytokine signal from positive conu·ol u·eated... Virus -Specific T Serum Fatigue Sta tus Sta tus ceU responses Cvtokines Score Cohort 1 14 Active Active X X X NIA Cohort 2 20 Active Previously r

  16. Towards Energy Efficiency: Forecasting Indoor Temperature via Multivariate Analysis

    Directory of Open Access Journals (Sweden)

    Juan Pardo

    2013-09-01

    Full Text Available The small medium large system (SMLsystem is a house built at the Universidad CEU Cardenal Herrera (CEU-UCH for participation in the Solar Decathlon 2013 competition. Several technologies have been integrated to reduce power consumption. One of these is a forecasting system based on artificial neural networks (ANNs, which is able to predict indoor temperature in the near future using captured data by a complex monitoring system as the input. A study of the impact on forecasting performance of different covariate combinations is presented in this paper. Additionally, a comparison of ANNs with the standard statistical forecasting methods is shown. The research in this paper has been focused on forecasting the indoor temperature of a house, as it is directly related to HVAC—heating, ventilation and air conditioning—system consumption. HVAC systems at the SMLsystem house represent 53:89% of the overall power consumption. The energy used to maintain temperature was measured to be 30%–38:9% of the energy needed to lower it. Hence, these forecasting measures allow the house to adapt itself to future temperature conditions by using home automation in an energy-efficient manner. Experimental results show a high forecasting accuracy and therefore, they might be used to efficiently control an HVAC system.

  17. Evaluating HapMap SNP data transferability in a large-scale genotyping project involving 175 cancer-associated genes.

    Science.gov (United States)

    Ribas, Gloria; González-Neira, Anna; Salas, Antonio; Milne, Roger L; Vega, Ana; Carracedo, Begoña; González, Emilio; Barroso, Eva; Fernández, Lara P; Yankilevich, Patricio; Robledo, Mercedes; Carracedo, Angel; Benítez, Javier

    2006-02-01

    One of the many potential uses of the HapMap project is its application to the investigation of complex disease aetiology among a wide range of populations. This study aims to assess the transferability of HapMap SNP data to the Spanish population in the context of cancer research. We have carried out a genotyping study in Spanish subjects involving 175 candidate cancer genes using an indirect gene-based approach and compared results with those for HapMap CEU subjects. Allele frequencies were very consistent between the two samples, with a high positive correlation (R) of 0.91 (PHapMap CEU data using pairwise r (2) thresholds of 0.8 and 0.5 was assessed by applying these to the Spanish and current HapMap data for 66 genes. In general, the HapMap tagSNPs performed very well. Our results show generally high concordance with HapMap data in allele frequencies and haplotype distributions and confirm the applicability of HapMap SNP data to the study of complex diseases among the Spanish population.

  18. Physical and genetic map of the Lactobacillus sakei 23K chromosome.

    Science.gov (United States)

    Dudez, Anne-Marie; Chaillou, Stéphane; Hissler, Lionel; Stentz, Régis; Champomier-Vergès, Marie-Christine; Alpert, Carl-Alfred; Zagorec, Monique

    2002-02-01

    The Lactobacillus sakei 23K chromosome was analysed by pulsed-field gel electrophoresis after digestion with the restriction enzymes AscI, NotI and SfiI. The chromosome size was estimated to be 1845+/-80 kb. The use of I-CeuI, specific for rrn genes encoding 23S rRNAs, showed that seven rrn loci were present, on 40% of the chromosome. The seven rrn clusters were mapped and their orientation was determined, allowing the position of the replication origin to be estimated. Partial I-CeuI digestions were used to construct a backbone and the different restriction fragments obtained with AscI, NotI and SfiI were assembled to a physical map by Southern hybridization. Eleven L. sakei gene clusters previously identified were mapped, as well as 25 new loci located randomly on the chromosome and 11 regions flanking the rrn gene clusters. A total of 47 clusters were thus mapped on L. sakei chromosome. The new loci were sequenced, allowing the identification of 73 complete or incomplete coding sequences. Among these 73 new genes of L. sakei, the function of 36 could be deduced from their similarity to known genes described in databases. However, 10 genes had no homologues, 10 encoded proteins similar to proteins of unknown function and 17 were similar to hypothetical proteins.

  19. Taming Many-Parameter BSM Models with Bayesian Neural Networks

    Science.gov (United States)

    Kuchera, M. P.; Karbo, A.; Prosper, H. B.; Sanchez, A.; Taylor, J. Z.

    2017-09-01

    The search for physics Beyond the Standard Model (BSM) is a major focus of large-scale high energy physics experiments. One method is to look for specific deviations from the Standard Model that are predicted by BSM models. In cases where the model has a large number of free parameters, standard search methods become intractable due to computation time. This talk presents results using Bayesian Neural Networks, a supervised machine learning method, to enable the study of higher-dimensional models. The popular phenomenological Minimal Supersymmetric Standard Model was studied as an example of the feasibility and usefulness of this method. Graphics Processing Units (GPUs) are used to expedite the calculations. Cross-section predictions for 13 TeV proton collisions will be presented. My participation in the Conference Experience for Undergraduates (CEU) in 2004-2006 exposed me to the national and global significance of cutting-edge research. At the 2005 CEU, I presented work from the previous summer's SULI internship at Lawrence Berkeley Laboratory, where I learned to program while working on the Majorana Project. That work inspired me to follow a similar research path, which led me to my current work on computational methods applied to BSM physics.

  20. [Analysis of the HapMap data on SNPs in SUMO1 and association study of rs7599810 in trios with non-syndromic cleft lip with or without cleft palate].

    Science.gov (United States)

    Yue, Qing; Wang, Hong; Zhang, Bo; Zhao, Kai-ping

    2014-04-18

    To analyze the minor allele frequencies (MAFs), haplotype block and haplotype frequencies for single nucleotide polymorphisms (SNPs) in SUMO1 using HapMap data and perform association analysis between SNPs in SUMO1 and non-syndromic cleft lip with or without cleft palate (NSCL/P) using 183 trios recruited from Shandong Province. SNPs with MAF>0.01 and Hardy-Weinberg equilibrium (P>0.01) were identified as qualified SNPs by Haploview. The MAFs of qualified SNPs were analyzed and the relative range ratios of the MAFs were developed to measure the differences in MAFs for common qualified SNPs with mutual minor allele among the samples. Haplotype blocks and haplotype frequencies for common qualified SNPs in four samples were analyzed and compared among the samples. Transmission disequilibrium test (TDT) was carried out to identify the association between rs7599810 and NSCL/P using 183 NSCL/P case-parent trios. Among the 24 SNPs released by HapMap project, the number of monotonic SNPs was 9 in Han Chinese in Beijing, China (CHB) and Japanese in Tokyo, Japan (JPT) samples, 8 in Utah residents with Northern and Western European ancestry from the CEPH collection (CEU) sample, and 6 in Yoruban in Ibadan, Nigeria (YRI) sample. The 12 common qualified SNPs in the four samples formed one haplotype in each sample. There were 3, 3, 5, and 6 forms of haplotypes in the CHB, JPT, CEU, and YRI samples respectively, and the cumulative frequencies for the 2 most common haplotypes ranged from 0.634 to 0.922. Significant transmission disequilibrium of G allele at rs7599810 from parents to offspring was not shown (χ(2)=0.485, P=0.486, OR=0.898, 95% CI: 0.663-1.021) for rs7599810 in Shandong Han sample. SNPs in SUMO1 mainly showed common features in minor alleles, MAFs and haplotype blocks among CHB, JPT and CEU samples, but unique features in YRI compared with those for the other three samples. Although no association was established between the markers in SUMO1 and NSCL/P using data

  1. Genetic variance in the spinocerebellar ataxia type 2 (ATXN2 gene in children with severe early onset obesity.

    Directory of Open Access Journals (Sweden)

    Karla P Figueroa

    Full Text Available BACKGROUND: Expansion of a CAG repeat in the coding region of exon 1 in the ATXN2 gene located in human chromosome 12q24.1 causes the neurodegenerative disease spinocerebellar ataxia type 2 (SCA2. In contrast to other polyglutamine (polyQ disorders, the SCA2 repeat is not highly polymorphic in central European (CEU controls with Q22 representing 90% of alleles, and Q23 contributing between 5-7% of alleles. Recently, the ATXN2 CAG repeat has been identified as a target of adaptive selection in the CEU population. Mouse lines deficient for atxn2 develop marked hyperphagia and obesity raising the possibility that loss-of-function mutations in the ATXN2 gene may be related to energy balance in humans. Some linkage studies of obesity related phenotypes such as antipsychotic induced weight gain have reported significant lod scores on chromosome 12q24. We tested the hypothesis that rare loss-of-function ATXN2 variants cause obesity analogous to rare mutations in the leptin, leptin receptor and MC4R genes. METHODOLOGY/PRINCIPAL FINDINGS: We sequenced the coding region of ATXN2 including intron-exon boundaries in 92 severely obese children with a body mass index (BMI >3.2 standard deviations above age- and gender-adjusted means. We confirmed five previously identified single nucleotide polymorphisms (SNPs and three new SNPs resulting in two synonymous substitutions and one intronic polymorphism. Alleles encoding >Q22 were overrepresented in our sample of obese children and contributed 15% of alleles in children identified by their parents as white. SNP rs695872 closely flanking the CAG repeat showed a greatly increased frequency of C/C homozygotes and G/C heterozygotes compared with reported frequencies in the CEU population. CONCLUSIONS/SIGNIFICANCE: Although we did not identify variants leading to novel amino acid substitutions, nonsense or frameshift mutations, this study warrants further examination of variation in the ATXN2 gene in obesity and

  2. Assessment of genotype imputation performance using 1000 Genomes in African American studies.

    Directory of Open Access Journals (Sweden)

    Dana B Hancock

    Full Text Available Genotype imputation, used in genome-wide association studies to expand coverage of single nucleotide polymorphisms (SNPs, has performed poorly in African Americans compared to less admixed populations. Overall, imputation has typically relied on HapMap reference haplotype panels from Africans (YRI, European Americans (CEU, and Asians (CHB/JPT. The 1000 Genomes project offers a wider range of reference populations, such as African Americans (ASW, but their imputation performance has had limited evaluation. Using 595 African Americans genotyped on Illumina's HumanHap550v3 BeadChip, we compared imputation results from four software programs (IMPUTE2, BEAGLE, MaCH, and MaCH-Admix and three reference panels consisting of different combinations of 1000 Genomes populations (February 2012 release: (1 3 specifically selected populations (YRI, CEU, and ASW; (2 8 populations of diverse African (AFR or European (AFR descent; and (3 all 14 available populations (ALL. Based on chromosome 22, we calculated three performance metrics: (1 concordance (percentage of masked genotyped SNPs with imputed and true genotype agreement; (2 imputation quality score (IQS; concordance adjusted for chance agreement, which is particularly informative for low minor allele frequency [MAF] SNPs; and (3 average r2hat (estimated correlation between the imputed and true genotypes, for all imputed SNPs. Across the reference panels, IMPUTE2 and MaCH had the highest concordance (91%-93%, but IMPUTE2 had the highest IQS (81%-83% and average r2hat (0.68 using YRI+ASW+CEU, 0.62 using AFR+EUR, and 0.55 using ALL. Imputation quality for most programs was reduced by the addition of more distantly related reference populations, due entirely to the introduction of low frequency SNPs (MAF≤2% that are monomorphic in the more closely related panels. While imputation was optimized by using IMPUTE2 with reference to the ALL panel (average r2hat = 0.86 for SNPs with MAF>2%, use of the ALL

  3. PCR detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from Danish pigs and cattle and cytolethal distending toxin production of the isolates

    DEFF Research Database (Denmark)

    Bang, Dang Duong; Nielsen, E.M.; Scheutz, F.

    2003-01-01

    among 40 C. jejuni and C. coli isolates was detected by polymerase chain reaction. The CDT production of the isolates was determined on Vero, colon 205 and chicken embryo cells. The cadF, flaA, ceuE and cdtB genes were detected from 100% of the isolates. The cdtA and cdtC genes were found in 95.0 and 90.......0% of the isolates, respectively. The cdt gene cluster was detected in 82.5% isolates. Only 7.5% of the isolates were positive for virB11. Ninety-five per cent of the isolates produced CDT in Vero and colon 205 cell assays, and 90% of the isolates produced CDT in chicken embryo cell assays. Conclusions: High...

  4. Real-time contrast-enhanced ultrasound determination of microvascular blood volume in abdominal subcutaneous adipose tissue in man. Evidence for adipose tissue capillary recruitment

    DEFF Research Database (Denmark)

    Tobin, L; Simonsen, L; Bülow, J

    2010-01-01

    The adipose tissue metabolism is dependent on its blood perfusion. During lipid mobilization e.g. during exercise and during lipid deposition e.g. postprandial, adipose tissue blood flow is increased. This increase in blood flow may involve capillary recruitment in the tissue. We investigated...... of ultrasound contrast agent to establish the reproducibility of the technique. In nine subjects, the effect of an oral glucose load on blood flow and microvascular volume was measured in abdominal subcutaneous adipose tissue and forearm skeletal muscle. ¹³³Xe washout and venous occlusion strain...... constant. It is concluded that the microvascular volume and changes in volume in abdominal subcutaneous adipose tissue can be assessed using CEU with good reproducibility. Postprandial capillary recruitment takes place in abdominal subcutaneous adipose tissue....

  5. Extending the reservoir of bla IMP-5: the emerging pathogen Acinetobacter bereziniae.

    Science.gov (United States)

    Grosso, Filipa; Silva, Liliana; Sousa, Clara; Ramos, Helena; Quinteira, Sandra; Peixe, Luísa

    2015-01-01

    Acinetobacter bereziniae clinical relevance is starting to be recognized; however, very few descriptions of its carbapenem resistance currently exist. Here we characterize two carbapenem-resistant A. bereziniae isolates. Isolates were obtained from environmental and clinical samples. Carbapenemases were searched by phenotypic, biochemical and PCR assays. Clonality was studied by ApaI-PFGE and genetic location for carbapenemase genes were assessed by I-CeuI and S1 hybridizations. Isolates were not clonally related but both produced the 'exclusively Portuguese' IMP-5, with the clinical isolate also producing an OXA-58. The carbapenemase genes were plasmid located. Our results emphasize the role of non-baumannii Acinetobacter species as important reservoirs of clinically relevant resistance genes that could also contribute to their emergence as nosocomial pathogens.

  6. Total mercury in muscles and liver of Mugil spp. from three coastal lagoons of NW Mexico: concentrations and risk assessment.

    Science.gov (United States)

    Delgado-Alvarez, C G; Frías-Espericueta, M G; Ruelas-Inzunza, J; Becerra-Álvarez, M J; Osuna-Martínez, C C; Aguilar-Juárez, M; Osuna-López, J I; Escobar-Sánchez, O; Voltolina, D

    2017-07-01

    Total mercury (Hg) concentrations were determined by atomic absorption spectrophotometry in muscles and liver of composite samples of Mugil cephalus and M. curema collected during November 2013 and in January, April, and July 2014 from the coastal lagoons Altata-Ensenada del Pabellón (AEP), Ceuta (CEU), and Teacapán-Agua Brava (TAG) of Sinaloa State. The mean Hg contents and information on local consumption were used to assess the possible risk caused by fish ingestion. Mean total mercury levels in the muscles ranged from 0.11 to 0.39 μg/g, while the range for liver was 0.12-3.91 μg/g. The mean Hg content of the liver was significantly (p mercury calculated for the younger age classes of one fishing community were >1, indicating a possible risk for some fishing communities of the Mexican Pacific coast.

  7. Life-Cycle Assessment of Prototype Unit of Emergency Housing. The search for the zero impact

    Directory of Open Access Journals (Sweden)

    J. M. Ros García

    2017-09-01

    Full Text Available Prototype Unit of Emergency Housing (PUEH is the result of the Applied Research Project VEM (Military Emergency Housing developed in collaboration with Escuela Politécnica Superior (Universidad CEU and the company Air-bus Defense & Space. It is designed as a modular and industrialized unit of basic habitability, with programmed and expandable growth, designed to provide shelter and protection in environments of humanitarian crises or contingencies of social vulnerability in order to ensure sustainable habitat for emergencies.The influence of the construction processes and materials involved in the manufacture of this PUEH have on the environment, analyzed using the methodology of life-cycle assessment (LCA, considered especially critical recycling the mate-rials used. Thus, in order to reduce the environmental impact environmental, each of the component parts of the developed prototype unit are quantified, evaluating the benefits resulting from the methodology DfMA (Design for Manufacturing and Assembly.

  8. Analysis of high-resolution HapMap of DTNBP1 (Dysbindin) suggests no consistency between reported common variant associations and schizophrenia.

    Science.gov (United States)

    Mutsuddi, Mousumi; Morris, Derek W; Waggoner, Skye G; Daly, Mark J; Scolnick, Edward M; Sklar, Pamela

    2006-11-01

    DTNBP1 was first identified as a putative schizophrenia-susceptibility gene in Irish pedigrees, with a report of association to common genetic variation. Several replication studies have reported confirmation of an association to DTNBP1 in independent European samples; however, reported risk alleles and haplotypes appear to differ between studies, and comparison among studies has been confounded because different marker sets were employed by each group. To facilitate evaluation of existing evidence of association and further work, we supplemented the extensive genotype data, available through the International HapMap Project (HapMap), about DTNBP1 by specifically typing all associated single-nucleotide polymorphisms reported in each of the studies of the Centre d'Etude du Polymorphisme Humain (CEPH)-derived HapMap sample (CEU). Using this high-density reference map, we compared the putative disease-associated haplotype from each study and found that the association studies are inconsistent with regard to the identity of the disease-associated haplotype at DTNBP1. Specifically, all five "replication" studies define a positively associated haplotype that is different from the association originally reported. We further demonstrate that, in all six studies, the European-derived populations studied have haplotype patterns and frequencies that are consistent with HapMap CEU samples (and each other). Thus, it is unlikely that population differences are creating the inconsistency of the association studies. Evidence of association is, at present, equivocal and unsatisfactory. The new dense map of the region may be valuable in more-comprehensive follow-up studies.

  9. Evaluation of HapMap data in six populations of European descent.

    Science.gov (United States)

    Lundmark, Per E; Liljedahl, Ulrika; Boomsma, Dorret I; Mannila, Heikki; Martin, Nicholas G; Palotie, Aarno; Peltonen, Leena; Perola, Markus; Spector, Tim D; Syvänen, Ann-Christine

    2008-09-01

    We studied how well the European CEU samples used in the Haplotype Mapping Project (HapMap) represent five European populations by analyzing nuclear family samples from the Swedish, Finnish, Dutch, British and Australian (European ancestry) populations. The number of samples from each population (about 30 parent-offspring trios) was similar to that in the HapMap sample sets. A panel of 186 single nucleotide polymorphisms (SNPs) distributed over the 1.5 Mb region of the GRID2 gene on chromosome 4 was genotyped. The genotype data were compared pair-wise between the HapMap sample and the other population samples. Principal component analysis (PCA) was used to cluster the data from different populations with respect to allele frequencies and to define the markers responsible for observed variance. The only sample with detectable differences in allele frequencies was that from Kuusamo, Finland. This sample also separated from the others, including the other Finnish sample, in the PCA analysis. A set of tagSNPs was defined based on the HapMap data and applied to the samples. The tagSNPs were found to capture the genetic variation in the analyzed region at r(2)>0.8 at levels ranging from 95% in the Kuusamo sample to 87% in the Australian sample. To capture the maximal genetic variation in the region, the Kuusamo, HapMap and Australian samples required 58, 63 and 73 native tagSNPs, respectively. The HapMap CEU sample represents the European samples well for tagSNP selection, with some caution regarding estimation of allele frequencies in the Finnish Kuusamo sample, and a slight reduction in tagging efficiency in the Australian sample.

  10. Antimicrobial Susceptibility and Genotypic Characteristic of Campylobacter spp. Isolates from Free-Living Birds in Poland.

    Science.gov (United States)

    Krawiec, Marta; Woźniak-Biel, Anna; Bednarski, Michał; Wieliczko, Alina

    2017-11-01

    Campylobacter spp. is the most commonly reported, bacterial cause of human foodborne infection worldwide. Commercial poultry and free-living birds are natural reservoirs of three particular species: Campylobacter jejuni, Campylobacter coli, and Campylobacter lari. The aim of this study was to determine the genotypic characteristics and antibiotic susceptibility of 43 Campylobacter strains, obtained from free-living birds, in Poland. In total, 700 birds were examined. The strains were isolated from 43 birds (6.14%) from the feces of 7 wild bird species: Mallard ducks Anas platyrhynchos (29 positive/121 tested), great cormorants Phalacrocorax carbo (5/77), velvet scoters Melanitta fusca (4/30), tawny owls Strix aluco (2/5), common buzzard Buteo buteo (1/3), rook Corvus frugilegus (1/6), and Eurasian tree sparrow Passer montanus (1/30). Thirty-eight (88.37%) of obtained strains belonged to C. jejuni and five (11.63%) to C. coli. Other 428 examined birds from different bird species were Campylobacter negative. The antimicrobial susceptibility to nine antimicrobials was also studied in investigated isolates of Campylobacter spp. Sixteen of the examined strains (37.21% of all positive samples) showed susceptibility to all of the nine antimicrobials. Moreover, the prevalence of selected virulence genes, such as flaA, cadF, ceuE, virB11, cdtA, cdtB, and cdtC were all analyzed. The virulence gene that was found most frequently in total number of Campylobacter strains was ceuE (72.10%) and other genes, such as flaA, cadF, cdtA, cdtB, and cdtC, were found in over 60% of all examined strains. Variable antimicrobial susceptibility and the presence of different virulence genes of examined strains, isolated from free-living birds, suggest that special attention should be given to wild birds and any potential approaches to the control of antibiotic-resistant Campylobacter should be discussed.

  11. Drug-resistant epilepsy: current recommendations for diagnosis and treatment in Spain.

    Science.gov (United States)

    Sánchez-Álvarez, J C; Gil-Nagel, A; Casas-Fernández, C; Mauri-Llerda, J A; Salas-Puig, J; Sancho-Rieger, J

    2012-01-01

    Drug-resistant epilepsy (DRE) is a top-priority social health problem which requires early individual treatment due to its dramatic repercussions for the patient and society. The International League Against Epilepsy (ILAE) has recently defined DRE as that in which the seizures are not controlled after having correctly taken two appropriate and well tolerated anti-epileptic drugs, with lack of control being understood as the appearance of seizures within one year or in a period less than three times the inter-seizure interval before starting treatment. This International Society recommends a rapid and detailed assessment of all patients in an Epilepsy Unit. A Clinical Epilepsy Unit (CEU) is understood as a group of professionals who, acting in collaboration, have the diagnosis and treatment of the patient with epilepsy as their primary objective. CEUs in Spain may be stratified into different levels depending on the activity carried out in each of them. The specific epilepsy clinic is considered the fundamental type of CEU and includes the necessary figure of an expert in epilepsy. Prolonged video-monitoring is performed in medical CEUs. In medical-surgical CEUs epilepsy surgery with varying degrees of difficulty is also performed. All CEUs must cooperate with consensus protocols, and there must be a two-way flow between them. Stratification of CEUs increases efficacy and efficiency, due to there being a sufficient number of them to ensure easy access by all patients with epilepsy. © 2011 Sociedad Española de Neurología. Published by Elsevier Espana. All rights reserved.

  12. Importance of pharmaceutical laboratory compliance with international standard requirements in respect of raising their competitiveness

    Directory of Open Access Journals (Sweden)

    Božanić Vojislav N.

    2009-01-01

    Full Text Available Current Good Manufacturing Practice (cGMP being a legal regulation in developed countries will become a legal regulation in Republic of Serbia starting with March 2010. In this paper comparative analysis between requirements of standard ISO/IEC 17025 and requirements of cEU GMP is shown. Considering the fact that in Republic of Serbia no pharmaceutical industry laboratory has been accredited according to requirements of ISO/IEC 17025, while keeping in mind that more than 90% of these laboratories have not fulfilled cEU GMP requirements, this paper aimed at pointing to the possibility of fulfilling both of mentioned requirements at the same time, which would open the way to different types of interlaboratory cooperation for pharmaceutical quality control laboratories and contribute to improving competitiveness of pharmaceutical companies. Accreditation, especially in the case of pharmaceutical quality control laboratories, is important because it guaranties the level of organizational and technical competency. It could easily be said that accreditation is becoming a must in quality control of products in order for the organization to be able to gain a leading role in the global market. Both accreditation and cGMP show the organization's commitment to having products of highest quality level. Considering the above mentioned facts, it is of greatest advantage for pharmaceutical quality control laboratories to fulfill both requirements of ISO/IEC 17025 and cGMP and reach total compliance. The aim of doing this lies in an easier acceptance of pharmaceutical products in different markets, overcoming technical barriers and affirmation of quality as key factor in reaching competitiveness, while keeping in mind the importance of strategic and competitive positioning in the global market.

  13. Characteristics and comparative performance of direct culture, direct PCR and enumeration methods for detection and quantification of Campylobacter spp. in broiler caeca.

    Science.gov (United States)

    Rodgers, J D; Lawes, J R; Vidal, A B; Ellis-Iversen, J; Ridley, A; Pleydell, E J; Powell, L F; Toszeghy, M; Stapleton, K; Clifton-Hadley, F A

    2012-10-12

    Detection and enumeration of Campylobacter spp. in broiler chicken flocks are key components of research and surveillance studies aimed at reducing Campylobacter infections in people. Direct culture of caecal contents onto selective agar is the typical method used to confirm flock colonisation. Modified charcoal cefoperazone deoxycholate agar (mCCDA) is commonly used for this method, although alternative selective media have been used. Additionally, PCR methods to detect Campylobacter DNA from caecal contents may provide a rapid alternative. However comparative performance data for these methods is limited and therefore required to ensure optimal detection methods for this sample type. In this study, 306 broiler caeca were tested for Campylobacter using direct culture on mCCDA, Skirrows and Preston agars and two real-time PCR methods, one specific for mapA/ceuE regions and another for the flaA gene region. Additionally, the suitability of spread plating and spiral plating methods for enumeration of Campylobacter and the impact of sample storage were assessed. This study confirmed modified CCDA as an optimal media for detection of Campylobacter in broiler caeca. It was significantly more sensitive than Skirrows or Preston agars. This study also demonstrated that the mapA/ceuE PCR had excellent agreement with culture on mCCDA and is a genuine alternative method. Spread plating and spiral plating methods were suitable for enumeration although spiral plating appeared more sensitive for stored samples (72 h). A 1 log reduction in viable Campylobacters was observed in stored samples, therefore storage effects should be considered for quantitative studies with broiler caeca. Crown Copyright © 2012. Published by Elsevier B.V. All rights reserved.

  14. Comparison of real-time PCR assays for detection, quantification, and differentiation of campylobacter jejuni and campylobacter coli in broiler neck skin samples.

    Science.gov (United States)

    Schnider, A; Overesch, G; Korczak, B M; Kuhnert, P

    2010-06-01

    We tested the use of multiplex real-time PCR for detection and quantification of Campylobacter jejuni and Campylobacter coli on broiler carcass neck skin samples collected during 2008 from slaughterhouses in Switzerland. Results from an established TaqMan assay based on two different targets (hipO and ceuE for C. jejuni and C. coli, respectively) were corroborated with data from a newly developed assay based on a single-nucleotide polymorphism in the fusA gene, which allows differentiation between C. jejuni and C. coli. Both multiplex real-time PCRs were applied simultaneously for direct detection, differentiation, and quantification of Campylobacter from 351 neck skin samples and compared with culture methods. There was good correlation in detection and enumeration between real-time PCR results and quantitative culture, with real-time PCR being more sensitive. Overall, 251 (71.5%) of the samples were PCR positive for Campylobacter, with 211 (60.1%) in the hipO-ceuE assays, 244 (69.5%) in the fusA assay, and 204 (58.1%) of them being positive in both PCR assays. Thus, the fusA assay was similarly sensitive to the enrichment culture (72.4% positive); however, it is faster and allows for quantification. In addition, real-time PCR allowed for species differentiation; roughly 60% of positive samples contained C. jejuni, less than 10% C. coli, and more than 30% contained both species. Real-time PCR proved to be a suitable method for direct detection, quantification, and differentiation of Campylobacter from carcasses, and could permit time-efficient surveillance of these zoonotic agents.

  15. Virulence genes and cytokine profile in systemic murine Campylobacter coli infection.

    Science.gov (United States)

    Klančnik, Anja; Pogačar, Maja Šikić; Raspor, Peter; Abram, Maja; Možina, Sonja Smole; Vučković, Darinka

    2015-01-01

    Campylobacter coli are one of the most common bacteria in bacterial gastroenteritis and acute enterocolitis in humans. However, relatively little is known regarding the mechanisms of pathogenesis and host response to C. coli infections. To investigate the influence of genetic changes, we first used PCR to demonstrate the presence of the known virulence genes cadF, virB11, cdtB, cdtC and ceuE in the clinical isolate C. coli 26536, which was isolated from the liver of infected BALB/c mice. Sequence analyses of the cadF, virB11, cdtB and ceuE genes in C. coli 26536 confirmed the stability in these virulence genes during their transmission through the host. We further investigated C. coli infection for the bacterial clearance from the liver and spleen of infected mice, and for their immune response. C. coli persisted well in both organs, with better survival in the liver. We also determined the levels of several pro-inflammatory cytokines (i.e., interleukin [IL]-6, IL-12, interferon-γ, tumor necrosis factor-α) and the anti-inflammatory cytokine IL-10 in plasma and in liver homogenates from the infected mice, using enzyme-linked immunosorbent assays. The lowest levels among these cytokines were for tumor necrosis factor-α in the plasma and IL-6 in the liver on days 1, 3 and 8 post-infection. The most pronounced production was for IL-10, in both plasma (days 1 and 8 post-infection) and liver (day 8 post-infection), which suggests that it has a role in healing of the organ inflammation. Our findings showed dynamic relationships between pro- and anti-inflammatory cytokines and thus contribute toward clarification of the healing processes involved in the resolution of C. coli infections.

  16. Genetic admixture and population substructure in Guanacaste Costa Rica.

    Directory of Open Access Journals (Sweden)

    Zhaoming Wang

    2010-10-01

    Full Text Available The population of Costa Rica (CR represents an admixture of major continental populations. An investigation of the CR population structure would provide an important foundation for mapping genetic variants underlying common diseases and traits. We conducted an analysis of 1,301 women from the Guanacaste region of CR using 27,904 single nucleotide polymorphisms (SNPs genotyped on a custom Illumina InfiniumII iSelect chip. The program STRUCTURE was used to compare the CR Guanacaste sample with four continental reference samples, including HapMap Europeans (CEU, East Asians (JPT+CHB, West African Yoruba (YRI, as well as Native Americans (NA from the Illumina iControl database. Our results show that the CR Guanacaste sample comprises a three-way admixture estimated to be 43% European, 38% Native American and 15% West African. An estimated 4% residual Asian ancestry may be within the error range. Results from principal components analysis reveal a correlation between genetic and geographic distance. The magnitude of linkage disequilibrium (LD measured by the number of tagging SNPs required to cover the same region in the genome in the CR Guanacaste sample appeared to be weaker than that observed in CEU, JPT+CHB and NA reference samples but stronger than that of the HapMap YRI sample. Based on the clustering pattern observed in both STRUCTURE and principal components analysis, two subpopulations were identified that differ by approximately 20% in LD block size averaged over all LD blocks identified by Haploview. We also show in a simulated association study conducted within the two subpopulations, that the failure to account for population stratification (PS could lead to a noticeable inflation in the false positive rate. However, we further demonstrate that existing PS adjustment approaches can reduce the inflation to an acceptable level for gene discovery.

  17. Classical rather than genetic risk factors account for high cardiovascular disease prevalence in Lithuania: A cross-sectional population study.

    Science.gov (United States)

    Burokienė, Neringa; Domarkienė, Ingrida; Ambrozaitytė, Laima; Uktverytė, Ingrida; Meškienė, Raimonda; Karčiauskaitė, Dovilė; Kasiulevičius, Vytautas; Šapoka, Virginijus; Kučinskas, Vaidutis; Kučinskienė, Zita Aušrelė

    2017-03-01

    Cardiovascular disease (CVD) mortality accounts for 54% of all deaths in Lithuania, making it the highest among all of the European Union countries. We evaluated the prevalence of several CVD risk factors, including lifestyle, blood biochemistry and genetic predisposition to determine the reasons behind significantly increased CVD prevalence in Lithuania. In total 435 volunteers of Lithuanian ethnicity and stable geographic settlement for 3 generations, had their anthropometric, biochemical and behavioural risk factors measured. A randomly selected sample of 166 volunteers had their 60 CVD risk alleles genotyped. The prevalence of risk alleles and cumulative CVD genetic risk score were compared with population of North-West European origin (CEU) using data from the phase 3 HapMap project. CVD was present in 33.8% of study volunteers, 84% of participants consumed alcohol, 21% were current smokers and only 30% of participants engaged in higher levels of physical activity. Also, the average BMI (males 28.3±4.3kg/m(2), females 27.3±5.0kg/m(2)), total cholesterol (males 6.1±1.2mmol/L, females 6.2±1.0mmol/L) and LDL-cholesterol (males 4.1±1.1mmol/L, females 4.1±1.0mmol/L) were above the normal values. The cumulative genetic susceptibility to develop CVD in Lithuanians was only 1.4% higher than in CEU population. High BMI and poor population plasma lipid profile are the major contributing factors to high CVD mortality and morbidity in Lithuania. Smoking, alcohol consumption and preliminary genetic predisposition results do not explain the difference in CVD mortality between the Lithuanian and wider European populations. CVD prevention programmes in Lithuania should primarily focus on weight loss and improving blood lipid control. Copyright © 2017 Medical University of Bialystok. Published by Elsevier B.V. All rights reserved.

  18. The Exiguobacterium genus: biodiversity and biogeography

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    Vishnivetskaya, Tatiana A [ORNL; Kathariou, Sophia [North Carolina State University; Tiedje, James M. [Michigan State University, East Lansing

    2009-01-01

    Abstract. Bacteria of the genus Exiguobacterium are low G + C, Gram-positive facultative anaerobes that have been repeatedly isolated from ancient Siberian permafrost. In addition, Exiguobacterium spp. have been isolated from markedly diverse sources, including Greenland Glacial ice, hot springs at Yellowstone National Park, the rhizosphere of plants, and the environment of food processing plants. Strains of this hereto little known bacterium that have been retrieved from such different (and often extreme) environments are worthy of attention as they are likely to be specifically adapted to such environments and to carry variations in the genome which may correspond to psychrophilic and thermophilic adaptations. However, comparative genomic investigations of Exiguobacterium spp. from different sources have been limited. In this study, we employed different molecular approaches for the comparative analysis of 24 isolates from markedly diverse environments including ancient Siberian permafrost and hot springs at Yellowstone National Park. Pulsed-field gel electrophoresis (PFGE) with I-CeuI (an intron-encoded endonuclease), AscI and NotI were optimized for the determination of genomic fingerprints of nuclease-producing isolates. The application of a DNA macroarray for 82 putative stress-response genes yielded strain-specific hybridization profiles. Cluster analyses of 16S rRNA gene sequence data, PFGE I-CeuI restriction patterns and hybridization profiles suggested that Exiguobacterium strains formed two distinct divisions that generally agreed with temperature ranges for growth. With few exceptions (e.g., Greenland ice isolate GIC31), psychrotrophic and thermophilic isolates belonged to different divisions.

  19. Calendar

    Science.gov (United States)

    2004-06-01

    JULY 2004 2nd World Congress of the Game Theory Society, Faculty of Luminy, Marseille, France 5-9 July 2004 Europa Organisation (europa@europa-organisation.com), +33 5 34 45 26 45, www.gts2004.org Budapest Workshop on Behavioral Economics, Central European University, Budapest, Hungary 5-10 July 2004 Eva Dotzi (behavecon@ceu.hu), www.iza.org/en/calls_conferences/CallCEU_04.pdf FDA'04. 1st IFAC Workshop on Fractional Differentiation and its Applications, Bordeaux, France 19-20 July 2004 IFAC secretariat (fda04@lap.u-bordeaux1.fr), www.lap.u-bordeaux.fr/fda04/ Bachelier Finance Society Third World Congress, InterContinental Hotel, Chicago, IL, USA 21-24 July 2004 bfs2004@uic.edu, www.uic.edu/orgs/bachelier/ BS/IMS 2004. 6th World Congress of the Bernoulli Society for Mathematical Statistics and Probability, Barcelona, Spain 26-31 July 2004 wc2004@pacifico-meetings.com, +34 93 402 13 85, www.imub.ub.es/events/wc2004 AUGUST 2004 Summer School in Econometrics. The Cointegrated VAR Model: Econometric Methodology and Macroeconomic Applications, Institute of Economics, University of Copenhagen, Denmark 2-22 August 2004 Summerschool@econ.ku.dk, www.econ.ku.dk/summerschool SEPTEMBER 2004 First Bonzenfreies Colloquium on Market Dynamics and Quantitative Economics, Alessandria, Palazzo Borsalino, Italy 9-10 September 2004 colloquium@unipmn.it, www.mfn.unipmn.it/~colloqui/ Risk Analysis 2004. 4th International Conference on Computer Simulation in Risk Analysis and Hazard Mitigation, Aldemar Paradise Royal Mare Hotel, Rhodes, Greece 27-29 September 2004 enquiries@wessex.ac.uk, +44 (0)238 029 3223, www.wessex.ac.uk/conferences/2004/risk04/ OCTOBER 2004 IRC Hedge 2004, InterContinental Hotel, London, UK 10, 11 October 2004 enquiries@irc-conferences.com, www.irc-conferences.com/show_conference.php?id=10 NOVEMBER 2004 IRC DICE 2004, InterContinental Hotel, London, UK 22, 23 November 2004 enquiries@irc-conferences.com, www.irc-conferences.com/show_conference.php?id=13 DECEMBER 2004

  20. Epidemiological association of Campylobacter jejuni groups with pathogenicity-associated genetic markers

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    Zautner Andreas E

    2012-08-01

    Full Text Available Abstract Background Campylobacter jejuni, the most leading cause for bacterial gastroenteritis worldwide, shows a high genetic diversity among its isolates. Recently, we demonstrated the existence of six C. jejuni-groups by combining MLST with six genetic markers. These groups were further characterized by the detection of cj1321-cj1326, fucP, cj0178, cj0755/cfrA, ceuE, pldA, cstII, and cstIII in order (I. to show further associations between these different genetic markers and MLST CCs. Moreover, different studies were able to associate several of these markers: a sialylated lipoologosaccharide (cstII/III+, the gamma-glytamyl-transpeptidase (ggt+, and the absence of a certain allele of the enterochelin-uptake-binding-protein (ceuE11168- with severe campylobacteriosis, bloody diarrhea and unpleasant outcome. Additionally more than half of human Campylobacter-isolates were assigned to a non-livestock clade associated with the absence of cj1321-cj1326. These isolates were considered as mere colonizers. From the combination of marker genes, the ratio of human isolates in a specific group, and clinical data (II. it should be demonstrated to which of the previous defined groups these Campylobacter-subpopulations, associated with higher virulence, correspond. Results Besides the marker gene pldA, all new estimated genetic markers show significant differences in their distribution among the various MLST-based groups. Especially the genes for cj1321-cj1326, fucP, cj0178, cj0755/cfrA are widely associated with each other and split the study population into two major and seven intermediate groups substantiating the previous group-definition, whereas cstII and cstIII indicate at least three groups following an independent distribution pattern. Conclusions Based on these data a group of C. jejuni-isolates characterized by the presence of ansB, dmsA, ggt, and the absence of cj1365c, cj1585c, cj1321-cj1326, fucP, cj0178, cj0755/cfrA, and cstII/III was

  1. Epidemiological association of Campylobacter jejuni groups with pathogenicity-associated genetic markers.

    Science.gov (United States)

    Zautner, Andreas E; Ohk, Carolin; Tareen, Abdul Malik; Lugert, Raimond; Gross, Uwe

    2012-08-08

    Campylobacter jejuni, the most leading cause for bacterial gastroenteritis worldwide, shows a high genetic diversity among its isolates. Recently, we demonstrated the existence of six C. jejuni-groups by combining MLST with six genetic markers. These groups were further characterized by the detection of cj1321-cj1326, fucP, cj0178, cj0755/cfrA, ceuE, pldA, cstII, and cstIII in order (I.) to show further associations between these different genetic markers and MLST CCs. Moreover, different studies were able to associate several of these markers: a sialylated lipoologosaccharide (cstII/III(+)), the gamma-glytamyl-transpeptidase (ggt(+)), and the absence of a certain allele of the enterochelin-uptake-binding-protein (ceuE(11168)(-)) with severe campylobacteriosis, bloody diarrhea and unpleasant outcome. Additionally more than half of human Campylobacter-isolates were assigned to a non-livestock clade associated with the absence of cj1321-cj1326. These isolates were considered as mere colonizers.From the combination of marker genes, the ratio of human isolates in a specific group, and clinical data (II.) it should be demonstrated to which of the previous defined groups these Campylobacter-subpopulations, associated with higher virulence, correspond. Besides the marker gene pldA, all new estimated genetic markers show significant differences in their distribution among the various MLST-based groups. Especially the genes for cj1321-cj1326, fucP, cj0178, cj0755/cfrA are widely associated with each other and split the study population into two major and seven intermediate groups substantiating the previous group-definition, whereas cstII and cstIII indicate at least three groups following an independent distribution pattern. Based on these data a group of C. jejuni-isolates characterized by the presence of ansB, dmsA, ggt, and the absence of cj1365c, cj1585c, cj1321-cj1326, fucP, cj0178, cj0755/cfrA, and cstII/III was associated with a higher prevalence in human

  2. Project ECHO (Extension for Community Healthcare Outcomes): A new model for educating primary care providers about treatment of substance use disorders

    Science.gov (United States)

    Komaromy, Miriam; Duhigg, Dan; Metcalf, Adam; Carlson, Cristina; Kalishman, Summers; Hayes, Leslie; Burke, Tom; Thornton, Karla; Arora, Sanjeev

    2016-01-01

    ABSTRACT Background: Project ECHO (Extension for Community Healthcare Outcomes) trains and mentors primary care providers (PCPs) in the care of patients with complex conditions. ECHO is a distance education model that connects specialists with numerous PCPs via simultaneous video link for the purpose of facilitating case-based learning. This article describes a teleECHO clinic based at the University of New Mexico Health Sciences Center that is focused on treatment of substance use disorders (SUDs) and behavioral health disorders. Methods: Since 2005, specialists in treatment of SUDs and behavioral health disorders at Project ECHO have offered a weekly 2-hour Integrated Addictions and Psychiatry (IAP) TeleECHO Clinic focused on supporting PCP evaluation and treatment of SUDs and behavioral health disorders. We tabulate the number of teleECHO clinic sessions, participants, and CME/CEU (continuing medical education/continuing education unit) credits provided annually. This teleECHO clinic has also been used to recruit physicians to participate in DATA-2000 buprenorphine waiver trainings. Using a database of the practice location of physicians who received the buprenorphine waiver since 2002, the number of waivered physicians per capita in US states was calculated. The increase in waivered physicians practicing in underserved areas in New Mexico was evaluated and compared with the rest of the United States. Results: Since 2008, approximately 950 patient cases have been presented during the teleECHO clinic, and more than 9000 hours of CME/CEU have been awarded. Opioids are the substances discussed most commonly (31%), followed by alcohol (21%) and cannabis (12%). New Mexico is near the top among US states in DATA-2000 buprenorphine-waivered physicians per capita, and it has had much more rapid growth in waivered physicians practicing in traditionally underserved areas compared with the rest of the United States since the initiation of the teleECHO clinic focused on

  3. Variabilidad del patrón respiratorio durante la carga elástica inspiratoria Variability of breathing pattern during inspiratory elastic load

    Directory of Open Access Journals (Sweden)

    Carlos E. D´Negri

    2009-06-01

    Full Text Available La ventilación pulmonar en humanos tiene una variabilidad respiración a respiración no lineal, compleja y caótica. El objetivo del trabajo fue: caracterizar la variabilidad del patrón respiratorio en perros (n: 8 anestesiados respirando bajo carga elástica umbral (CEU inspiratoria (7 a 50 cm H2O. Con el flujo, presión traqueal y esofágica, se analizaron: tiempo inspiratorio (Ti, ritmo [tiempo espiratorio (Te; tiempo total (Ttot, y Ti/Ttot] e impulso central (Vt/Ti, variables relacionadas [volumen corriente (Vt y ventilación pulmonar (Ve]. Se determinaron: variabilidad grosera (varianzas, oscilaciones de baja frecuencia (análisis espectral y memoria a corto plazo (análisis de autocorrelación. La CEU produjo disminución de la varianza de medias en Te, Ttot, Vt y Vt/Ti (p In humans, lung ventilation exhibits breath-to-breath variability and dynamics that are nonlinear, complex and chaotic. Our objective was to characterize the breathing pattern variational activity in anesthetized dogs (n: 8 breathing through threshold inspiratory elastic load (7 to 50 cm H2O. Starting from flow signal and tracheal and esophageal pressures, we analyzed inspiratory time (Ti, timing (expiratory time, Te; total time, Ttot; and Ti/Ttot and central drive (Vt/Ti and variables related to it (tidal volume, Vt and pulmonary ventilation, Ve. We measured gross variability (variances, low frequency oscillations (spectral analysis, and short term memory (autocorrelation analysis. Loading decreased variance of the mean values of Te, Ttot, Vt and Vt/Ti (p < 0.05; the mean of variances for Ti/Ttot increased (p < 0.005 while it decreased for Vt and Vt/Ti (p < 0.05. In general, percent of data recordings with low frequency oscillations (OB% decreased (p < 0.02. During heavy load, timing parameters percent of data recordings with autocorrelations (AU% did not change, but Vt and its related parameters decreased their AU% (p < 0.005. There was a positive correlation (r: 0

  4. Genetic comparison of a Croatian isolate and CEPH European founders.

    Science.gov (United States)

    Navarro, Pau; Vitart, Véronique; Hayward, Caroline; Tenesa, Albert; Zgaga, Lina; Juricic, Danica; Polasek, Ozren; Hastie, Nicholas D; Rudan, Igor; Campbell, Harry; Wright, Alan F; Haley, Chris S; Knott, Sara A

    2010-02-01

    Human isolates have been postulated as a good resource for the identification of QTL due to reduced genetic diversity and a more homogeneous environment. Isolates may also have increased linkage disequilibrium (LD) due to small effective population size and, either loss or increase in frequency of alleles that are rare in the general population from which they originate. Here we investigate the difference in allele and genotype frequencies, LD and homozygous tracts between an isolate-several villages from the island of Vis in Croatia-and an outbred population of European origin: the Hapmap CEPH founders. Using the HumanHap300 v1 Genotyping BeadChip, we show that our population does not differ greatly from the reference CEU outbred population despite having a slightly higher proportion of monomorphic loci, a slightly higher long-range LD, and a greater proportion of individuals with long homozygous tracts. We conclude that genotyping arrays should perform equally well in our isolate as in outbred European populations for disease mapping studies and that SNP-trait associations discovered in our well-characterized Croatian isolate should be valid in the general European population from which they descend. 2009 Wiley-Liss, Inc.

  5. Social Scientists on Ethnic Relations in Croatia after 1989: Content Analysis of an Annotated Bibliography

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    Árpád Baráth

    2000-06-01

    Full Text Available The article presents the main results of qualitative analysis of an annotated bibliography on ethnic relations in Croatia after 1989, prepared by the CEU, South-Eastern European Studies Department (Budapest. The bibliography was a selected sample of academic literature published, with few exceptions, by Croatian social and political scientists between 1989 and 1998. Five major classification criteria were found that “best” describe the mainstream of academic interest in-, and for Croatia during the past decade. These are issues dealing with: (1 ethnic identity, (2 nationalism & ethnic conflicts, (3 war & related human catastrophes, (4 human, ethnic & minority rights, (5 multiculturalism & democracy. The content analysis of abstracts and listed key words uncovered a significant shift of interest of Croatian scholars during the past 10 years from issues on ethnic identity & conflicts toward issues of multiculturalism & human rights. With the exception of few titles, the reviewer found the annotated bibliography complete with customary library search data, representative for a wide range of disciplines and balanced in selection.

  6. Performance of genotype imputations using data from the 1000 Genomes Project.

    Science.gov (United States)

    Sung, Yun Ju; Wang, Lihua; Rankinen, Tuomo; Bouchard, Claude; Rao, D C

    2012-01-01

    Genotype imputations based on 1000 Genomes (1KG) Project data have the advantage of imputing many more SNPs than imputations based on HapMap data. It also provides an opportunity to discover associations with relatively rare variants. Recent investigations are increasingly using 1KG data for genotype imputations, but only limited evaluations of the performance of this approach are available. In this paper, we empirically evaluated imputation performance using 1KG data by comparing imputation results to those using the HapMap Phase II data that have been widely used. We used three reference panels: the CEU panel consisting of 120 haplotypes from HapMap II and 1KG data (June 2010 release) and the EUR panel consisting of 566 haplotypes also from 1KG data (August 2010 release). We used Illumina 324,607 autosomal SNPs genotyped in 501 individuals of European ancestry. Our most important finding was that both 1KG reference panels provided much higher imputation yield than the HapMap II panel. There were more than twice as many successfully imputed SNPs as there were using the HapMap II panel (6.7 million vs. 2.5 million). Our second most important finding was that accuracy using both 1KG panels was high and almost identical to accuracy using the HapMap II panel. Furthermore, after removing SNPs with MACH Rsq Project is still underway, we expect that later versions will provide even better imputation performance. Copyright © 2011 S. Karger AG, Basel.

  7. HapMap SNP Scanner: an online program to mine SNPs responsible for cell phenotype.

    Science.gov (United States)

    Yamamura, T; Hikita, J; Bleakley, M; Hirosawa, T; Sato-Otsubo, A; Torikai, H; Hamajima, T; Nannya, Y; Demachi-Okamura, A; Maruya, E; Saji, H; Yamamoto, Y; Takahashi, T; Emi, N; Morishima, Y; Kodera, Y; Kuzushima, K; Riddell, S R; Ogawa, S; Akatsuka, Y

    2012-08-01

    Minor histocompatibility (H) antigens are targets of graft-vs-host disease and graft-vs-tumor responses after human leukocyte antigen matched allogeneic hematopoietic stem cell transplantation. Recently, we reported a strategy for genetic mapping of linkage disequilibrium blocks that encoded novel minor H antigens using the large dataset from the International HapMap Project combined with conventional immunologic assays to assess recognition of HapMap B-lymphoid cell line by minor H antigen-specific T cells. In this study, we have constructed and provide an online interactive program and demonstrate its utility for searching for single-nucleotide polymorphisms (SNPs) responsible for minor H antigen generation. The website is available as 'HapMap SNP Scanner', and can incorporate T-cell recognition and other data with genotyping datasets from CEU, JPT, CHB, and YRI to provide a list of candidate SNPs that correlate with observed phenotypes. This method should substantially facilitate discovery of novel SNPs responsible for minor H antigens and be applicable for assaying of other specific cell phenotypes (e.g. drug sensitivity) to identify individuals who may benefit from SNP-based customized therapies. © 2012 John Wiley & Sons A/S.

  8. Semantic Modeling for SNPs Associated with Ethnic Disparities in HapMap Samples.

    Science.gov (United States)

    Kim, Hyoyoung; Yoo, Won Gi; Park, Junhyung; Kim, Heebal; Kang, Byeong-Chul

    2014-03-01

    Single-nucleotide polymorphisms (SNPs) have been emerging out of the efforts to research human diseases and ethnic disparities. A semantic network is needed for in-depth understanding of the impacts of SNPs, because phenotypes are modulated by complex networks, including biochemical and physiological pathways. We identified ethnicity-specific SNPs by eliminating overlapped SNPs from HapMap samples, and the ethnicity-specific SNPs were mapped to the UCSC RefGene lists. Ethnicity-specific genes were identified as follows: 22 genes in the USA (CEU) individuals, 25 genes in the Japanese (JPT) individuals, and 332 genes in the African (YRI) individuals. To analyze the biologically functional implications for ethnicity-specific SNPs, we focused on constructing a semantic network model. Entities for the network represented by "Gene," "Pathway," "Disease," "Chemical," "Drug," "ClinicalTrials," "SNP," and relationships between entity-entity were obtained through curation. Our semantic modeling for ethnicity-specific SNPs showed interesting results in the three categories, including three diseases ("AIDS-associated nephropathy," "Hypertension," and "Pelvic infection"), one drug ("Methylphenidate"), and five pathways ("Hemostasis," "Systemic lupus erythematosus," "Prostate cancer," "Hepatitis C virus," and "Rheumatoid arthritis"). We found ethnicity-specific genes using the semantic modeling, and the majority of our findings was consistent with the previous studies - that an understanding of genetic variability explained ethnicity-specific disparities.

  9. Evaluating the performance of commercial whole-genome marker sets for capturing common genetic variation

    Science.gov (United States)

    Mägi, Reedik; Pfeufer, Arne; Nelis, Mari; Montpetit, Alexandre; Metspalu, Andres; Remm, Maido

    2007-01-01

    Background New technologies have enabled genome-wide association studies to be conducted with hundreds of thousands of genotyped SNPs. Several different first-generation genome-wide panels of SNPs have been commercialized. The total amount of common genetic variation is still unknown; however, the coverage of commercial panels can be evaluated against reference population samples genotyped by the International HapMap project. Less information is available about coverage in samples from other populations. Results In this study we compare four commercial panels: the HumanHap 300 and HumanHap 550 Array Sets from the Illumina Infinium series and the Mapping 100 K and Mapping 500 K Array Sets from the Affymetrix GeneChip series. Tagging performance is compared among HapMap CEPH (CEU), Asian (JPT, CHB) and Yoruba (YRI) population samples. It is also evaluated in an Estonian population sample with more than 1000 individuals genotyped in two 500-kbp ENCODE regions of chromosome 2: ENr112 on 2p16.3 and ENr131 on 2p37.1. Conclusion We found that in a non-reference Caucasian population, commercial SNP panels provide levels of coverage similar to those in the HapMap CEPH population sample. We present the proportions of universal and population-specific SNPs in all the commercial platforms studied. PMID:17562002

  10. Métodos de Decisión : Asignatura integradora de Matemáticas y Estadística

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    López Zafra, J.M.

    1998-01-01

    Full Text Available La asignatura de Métodos de Decisión, obligatoria en los planes de estudio de la Licenciatura en ADE de determinadas facultades de CCEE como la de la Universidad Pontificia Comillas, la Universidad Complutense o la Universidad San Pablo - CEU (en esta última, bajo el nombre de Teoría y Métodos de Decisión, requiere por parte del alumno un manejo adecuado de las asignaturas anteriores relacionadas con las matemáticas y la estadística. Los tres ambientes en los que se desarrollan los procesos de decisión (certeza, riesgo e incertidumbre tienen peculiaridades propias que provocan el empleo constante de las técnicas habituales de la investigación operativa. En el presente trabajo se pretende precisamente señalar cómo la asignatura de referencia enfoca los aspectos relevantes de los métodos matemático-estadísticos y cómo permite un desarrollo integrador de las disciplinas anteriormente descritas.

  11. Chromosome map of Xanthomonas campestris pv. campestris 17 with locations of genes involved in xanthan gum synthesis and yellow pigmentation.

    Science.gov (United States)

    Tseng, Y H; Choy, K T; Hung, C H; Lin, N T; Liu, J Y; Lou, C H; Yang, B Y; Wen, F S; Weng, S F; Wu, J R

    1999-01-01

    No plasmid was detected in Xanthomonas campestris pv. campestris 17, a strain of the causative agent of black rot in cruciferous plants isolated in Taiwan. Its chromosome was cut by PacI, PmeI, and SwaI into five, two, and six fragments, respectively, and a size of 4.8 Mb was estimated by summing the fragment lengths in these digests. Based on the data obtained from partial digestion and Southern hybridization using probes common to pairs of the overlapping fragments or prepared from linking fragments, a circular physical map bearing the PacI, PmeI, and SwaI sites was constructed for the X. campestris pv. campestris 17 chromosome. Locations of eight eps loci involved in exopolysaccharide (xanthan gum) synthesis, two rrn operons each possessing an unique I-CeuI site, one pig cluster required for yellow pigmentation, and nine auxotrophic markers were determined, using mutants isolated by mutagenesis with Tn5(pfm)CmKm. This transposon contains a polylinker with sites for several rare-cutting restriction endonucleases located between the chloramphenicol resistance and kanamycin resistance (Kmr) genes, which upon insertion introduced additional sites into the chromosome. The recA and tdh genes, with known sequences, were mapped by tagging with the polylinker-Kmr segment from Tn5(pfm)CmKm. This is the first map for X. campestris and would be useful for genetic studies of this and related Xanthomonas species.

  12. The winding road to being a code monkey

    Science.gov (United States)

    Sarahan, Michael

    2017-09-01

    I am now a software engineer at a company that provides data analytics services, and helps support the open source data science community. I have been a computer nerd for a very long time, but it was my CEU experience at Texas A&M with Sherry Yennello (2003-2005) that helped me put my nerd skills to productive use. My project then was simulation of pulse shape discrimination electronics, and it was an excellent introduction to core computational concerns, such as digitization: when you see a line on the screen, that's not really how the computer sees it. I wandered in graduate school through a chemistry program into using electron microscopes. My programming interest got me into image and signal processing, which led naturally to jobs in analyzing data, and also in acquiring data. Throughout, it was always difficult just to make software work. I got pretty good at making it work. That's what I do for a living now - package software so that it is easy for other people to do great science with.

  13. Actividades emprendedoras y espacios alternativos de aprendizaje. Desarrollo de competencias durante un hackathon day

    Directory of Open Access Journals (Sweden)

    Carmen Ruiz Viñals

    2017-05-01

    Full Text Available El proyecto de los espacios sociales de aprendizaje en la Universidad Española se inició como parte del cambio de modelo educativo propuesto por el proceso de Bolonia. Dichos espacios los conforman todos los lugares que no son los aularios y que ayudan a completar el aprendizaje formal. En la presente investigación se reflexiona sobre estos espacios y las actividades que se desarrollan en ellos, donde los estudiantes adquieren las competencias y habilidades más demandadas por el mercado laboral, especialmente las llamadas competencias emprendedoras. Anualmente se organiza un maratón de emprendimiento llamado Barcelona Thinking Challenge en la UAO CEU. Dicha actividad es un espacio de aprendizaje donde los estudiantes adquieren competencias transversales y específicas, trabajando en equipos formados por estudiantes de diferentes cursos, universidades y profesionales. Se analizan los resultados de un estudio de encuesta que valora cuál ha sido la visión general de este maratón de emprendimiento social y qué competencias emprendedoras ha permitido desarrollar en los participantes. Los resultados permiten destacar que los partícipes valoran positivamente esta actividad emprendedora. Se muestra un alto desarrollo en las competencias relacionadas con trabajar en equipo, liderazgo y aplicación de conocimientos adquiridos en la universidad a la resolución de problemas.

  14. Analysis of DNA variations in GSTA and GSTM gene clusters based on the results of genome-wide data from three Russian populations taken as an example

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    Filippova Irina N

    2012-10-01

    Full Text Available Abstract Background Extensive genome-wide analyses of many human populations, using microarrays containing hundreds of thousands of single-nucleotide polymorphisms, have provided us with abundant information about global genomic diversity. However, these data can also be used to analyze local variability in individual genomic regions. In this study, we analyzed the variability in two genomic regions carrying the genes of the GSTA and GSTM subfamilies, located on different chromosomes. Results Analysis of the polymorphisms in GSTA and GSTM gene clusters showed similarities in their allelic and haplotype diversities. These patterns were similar in three Russian populations and the CEU population of European origin. There were statistically significant differences in all the haploblocks of both the GSTM and GSTA regions when the Russian populations were compared with populations from China and Japan. Most haploblocks also differed between the Russians and Nigerians from Yoruba, but, some of them had similar allelic frequencies. Special attention was paid to SNP rs4986947 from the intron of the GSTA4 gene, which is represented in apes by an A nucleotide. In the Asian and African samples, it was represented only by a G allele, and both allelic variants (G/A occurred in the Russian and European populations. Conclusions The results obtained suggest the presence of common features in the evolutionary histories of the GSTA and GSTM gene regions, and that African subpopulations were involved differently in the formation of the European and Asian human lineages.

  15. Active rollback in the Gibraltar Arc: Evidences from CGPS data in the western Betic Cordillera

    Science.gov (United States)

    Gonzalez-Castillo, L.; Galindo-Zaldivar, J.; de Lacy, M. C.; Borque, M. J.; Martinez-Moreno, F. J.; García-Armenteros, J. A.; Gil, A. J.

    2015-11-01

    The Gibraltar Arc, located in the western Mediterranean Sea, is an arcuate Alpine orogen formed by the Betic and Rif Cordilleras, separated by the Alboran Sea. New continuous GPS data (2008-2013) obtained in the Topo-Iberia stations of the western Betic Cordillera allow us to improve the present-day deformation pattern related to active tectonics in this collision area between the Eurasian and African plates. These data indicate a very consistent westward motion of the Betic Cordillera with respect to the relatively stable Iberian Massif foreland. The displacement in the Betics increases toward the south and west, reaching maximum values in the Gibraltar Strait area (4.27 mm/yr in Ceuta, CEU1, and 4.06 mm/yr in San Fernando, SFER), then progressively decreasing toward the northwestern mountain front. The recent geological structures and seismicity evidence moderate deformation in a roughly NW-SE to WNW-ESE compressional stress setting in the mountain frontal areas, and moderate extension toward the internal part of the cordillera. The mountain front undergoes progressive development of folds affecting at least up to Pliocene deposits, with similar recent geological and geodetical rates. This folded strip helps to accommodate the active deformation with scarce associated seismicity. The displacement pattern is in agreement with the present-day clockwise rotation of the tectonic units in the northern branch of the Gibraltar Arc. Our data support that the westward emplacement of the Betic Cordillera continues to be active in a rollback tectonic scenario.

  16. Occurrence of Rare Tree and Shrub Species in Hungary

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    BARTHA, Dénes

    2005-01-01

    Full Text Available The Department of Botany has been focusing on investigating rare taxa of theHungarian dendroflora since 1989. The research dealt with nearly 50 species regarding conditions of occurrence, habitat preference, reproduction and possible conservational management up to 2003. In the investigation of species, chorology was of primary importance. Since 1993 occurrence data have been systematically collected. As a first result, CEU grid-maps of 142 species, including all the rare dendrotaxa in Hungary, were published (Bartha – Mátyás 1995 using grid squares of 10' long. × 6' lat. equalling approx. 12 × 11 km. With organizational and methodological experience, focuses have moved to processing, correction and mapping of reference, herbaria and new field records. A detailed evaluation of the distribution of 34 rare species in Hungary was made and published (Bartha et al 1999. The Department of Botany at the University of West Hungary has been project coordinator of ‘Floristic Mapping of Hungary’ since 2001. The present study describes actual distribution maps of 20 rare tree- and shrub species with short analyses of their conditions in Hungary.

  17. In-silico analysis of inflammatory bowel disease (IBD GWAS loci to novel connections.

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    Md Mesbah-Uddin

    Full Text Available Genome-wide association studies (GWASs for many complex diseases, including inflammatory bowel disease (IBD, produced hundreds of disease-associated loci-the majority of which are noncoding. The number of GWAS loci is increasing very rapidly, but the process of translating single nucleotide polymorphisms (SNPs from these loci to genomic medicine is lagging. In this study, we investigated 4,734 variants from 152 IBD associated GWAS loci (IBD associated 152 lead noncoding SNPs identified from pooled GWAS results + 4,582 variants in strong linkage-disequilibrium (LD (r2 ≥0.8 for EUR population of 1K Genomes Project using four publicly available bioinformatics tools, e.g. dbPSHP, CADD, GWAVA, and RegulomeDB, to annotate and prioritize putative regulatory variants. Of the 152 lead noncoding SNPs, around 11% are under strong negative selection (GERP++ RS ≥2; and ~30% are under balancing selection (Tajima's D score >2 in CEU population (1K Genomes Project--though these regions are positively selected (GERP++ RS <0 in mammalian evolution. The analysis of 4,734 variants using three integrative annotation tools produced 929 putative functional SNPs, of which 18 SNPs (from 15 GWAS loci are in concordance with all three classifiers. These prioritized noncoding SNPs may contribute to IBD pathogenesis by dysregulating the expression of nearby genes. This study showed the usefulness of integrative annotation for prioritizing fewer functional variants from a large number of GWAS markers.

  18. La investigación operativa en las licenciaturas de economia y de administración de empresas

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    Raquel Ibar Alonso

    2002-01-01

    Full Text Available En las licenciaturas de Economía y de Administración y Dirección de Empresas se adquieren los conocimientos teóricos suficientes para ser un profesional competente. Sin embargo, en muchas ocasiones no se encuentra la forma óptima de modelizar la realidad estudiada, lo que complica su estudio. La asignatura “Investigación Operativa” proporciona una forma lógica de abordar los problemas de carácter económico, facilitando la formulación matemática más adecuada para representar, en cada caso, el problema estudiado. En el desarrollo de esta asignatura, según se explica en la USP-CEU, se plantean y resuelven problemas tipo utilizando el ordenador como herramienta de ayuda. El paquete informático que se emplea es el WINQSB, ya que es un programa que resulta muy sencillo de manejar a la vez que dispone de diversos módulos a través de los que se resuelven la mayoría de los problemas que nos encontramos cuando nos introducimos en el mundo económico y empresarial.

  19. Semantic Modeling for SNPs Associated with Ethnic Disparities in HapMap Samples

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    HyoYoung Kim

    2014-03-01

    Full Text Available Single-nucleotide polymorphisms (SNPs have been emerging out of the efforts to research human diseases and ethnic disparities. A semantic network is needed for in-depth understanding of the impacts of SNPs, because phenotypes are modulated by complex networks, including biochemical and physiological pathways. We identified ethnicity-specific SNPs by eliminating overlapped SNPs from HapMap samples, and the ethnicity-specific SNPs were mapped to the UCSC RefGene lists. Ethnicity-specific genes were identified as follows: 22 genes in the USA (CEU individuals, 25 genes in the Japanese (JPT individuals, and 332 genes in the African (YRI individuals. To analyze the biologically functional implications for ethnicity-specific SNPs, we focused on constructing a semantic network model. Entities for the network represented by "Gene," "Pathway," "Disease," "Chemical," "Drug," "ClinicalTrials," "SNP," and relationships between entity-entity were obtained through curation. Our semantic modeling for ethnicity-specific SNPs showed interesting results in the three categories, including three diseases ("AIDS-associated nephropathy," "Hypertension," and "Pelvic infection", one drug ("Methylphenidate", and five pathways ("Hemostasis," "Systemic lupus erythematosus," "Prostate cancer," "Hepatitis C virus," and "Rheumatoid arthritis". We found ethnicity-specific genes using the semantic modeling, and the majority of our findings was consistent with the previous studies - that an understanding of genetic variability explained ethnicity-specific disparities.

  20. Genetic variation in key genes associated with statin therapy in the Azores Islands (Portugal) healthy population.

    Science.gov (United States)

    Melo, Mafalda S; Balanco, Leticia; Branco, Claudia C; Mota-Vieira, Luisa

    2015-01-01

    Inter-individual variation in response to statins (efficacy and toxicity) has been described and may be due to polymorphisms implicated in drug pharmacokinetics or pharmacodynamics. This study investigates clinically relevant pharmacogenes underlying statin response in 170 healthy Azoreans. Eight SNPs in candidate genes-HMGCR (rs3846662, rs17238540, rs17244841), CETP (rs708272), APOE (rs7412, rs429358) and SLCO1B1 (rs2306283, rs4149056)-were genotyped. The allele frequencies were similar to those reported for European derived populations, excepting SLCO1B1 c.388A>G (rs2306283), which has a significant difference when compared with the HapMap CEU population (p = 1 × 10(-8)). The results of statin efficacy showed that 9.1% of Azoreans are APOE4 carriers. This allele has been associated with lower LDLc reduction from statin therapy and also higher LDLc levels at baseline. Regarding SLCO1B1, associated with statin toxicity, 1.8% of individuals have two reduced-function alleles (c.521CC). The results contribute to overcome the lack of knowledge regarding the frequency of pharmacogenetic SNPs and their corresponding haplotypes in targeted populations, such as Azores islands. Moreover, the present work constitutes an initial step to implementing pharmacogenomics in clinical practice where physicians could use a patient's genetic make-up to optimize statin therapy, regarding efficiency and myopathy risk.

  1. Matemáticas de las operaciones financieras: Una propuesta de programa.

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    Mª Carmen Escribano Ródenas

    2004-01-01

    Full Text Available Las asignaturas de Matemáticas de las Operaciones Financieras se imparten en prácticamente todas las licenciaturas de Administración y Dirección de Empresas, y Ciencias Económicas de las universidades españolas, sin embargo, su carácter obligatorio u optativo, sus contenidos, metodología, bibliografía, y número de horas lectivas son completamente diferentes y diversos. En la Universidad San Pablo-CEU de Madrid, en el nuevo plan de estudios, esta asignatura se imparte tanto en la licenciatura en CC. Económicas, como en la de Administración y Dirección de Empresas, con un carácter obligatorio para todos los alumnos. El objeto de este trabajo es presentar un programa adaptado para esta asignatura, con unos contenidos tradicionales y a la vez innovadores, que ya lleva funcionando algunos años y por lo tanto se pueden extraer conclusiones de su aplicación.

  2. Genome-wide mapping of copy number variation in humans: comparative analysis of high resolution array platforms.

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    Rajini R Haraksingh

    Full Text Available Accurate and efficient genome-wide detection of copy number variants (CNVs is essential for understanding human genomic variation, genome-wide CNV association type studies, cytogenetics research and diagnostics, and independent validation of CNVs identified from sequencing based technologies. Numerous, array-based platforms for CNV detection exist utilizing array Comparative Genome Hybridization (aCGH, Single Nucleotide Polymorphism (SNP genotyping or both. We have quantitatively assessed the abilities of twelve leading genome-wide CNV detection platforms to accurately detect Gold Standard sets of CNVs in the genome of HapMap CEU sample NA12878, and found significant differences in performance. The technologies analyzed were the NimbleGen 4.2 M, 2.1 M and 3×720 K Whole Genome and CNV focused arrays, the Agilent 1×1 M CGH and High Resolution and 2×400 K CNV and SNP+CGH arrays, the Illumina Human Omni1Quad array and the Affymetrix SNP 6.0 array. The Gold Standards used were a 1000 Genomes Project sequencing-based set of 3997 validated CNVs and an ultra high-resolution aCGH-based set of 756 validated CNVs. We found that sensitivity, total number, size range and breakpoint resolution of CNV calls were highest for CNV focused arrays. Our results are important for cost effective CNV detection and validation for both basic and clinical applications.

  3. Editorial 2: La Nueva Teoría Estratégica y sus desarrollos en los ámbitos comunicacionales / The New Strategic Theory and its developments in the communication area

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    María Isabel de Salas Nestares

    2014-12-01

    Full Text Available Cuando en el año 2008, con ocasión del Congreso Internacional Brand Trends, organizado por la Universidad CEU Cardenal Herrera en Valencia, me comentó el profesor Rafael Alberto Pérez cómo había visto la aplicación de la Nueva Teoría Estratégica en las diferentes conferencias de los profesionales de comunicación comercial y corporativa allí reunidos, tomé conciencia de que efectivamente la NTE se estaba ya aplicando en el campo de la Publicidad y el Marketing, validando así el modelo o paradigma. Y por otro lado me reafirmé en mi consideración de que el proceso tecnológico de digitalización de la comunicación ha sido el magma sobre el cual la NTE ha podido avanzar en su postulado relacional. A partir de entonces seguí su consejo sobre la necesidad de realizar un cambio en “la mirada” de cómo contemplar el mundo y hacerlo rompiendo con la mirada heredada de un mundo fragmentado, con causalidad lineal y estático y pasar a una visión compleja, fluida y caótica (Pérez y Massoini, 2009: 137-142.

  4. Spanish medical students' attitudes and views towards mental health and psychiatry: a multicentric cross-sectional study.

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    Failde, Inmaculada; Salazar, Alejandro; Elorza, Julian; Casais, Leonardo; Pérez, Víctor; Martínez, Luis Caballero; Gilaberte, Inmaculada

    2014-06-01

    The aim of this study is to investigate the attitudes towards mental illness and psychiatry among fifth year Spanish medical students. The study included 171 students from three medical schools located in different areas of Spain: Cádiz; UCA (n = 113), Madrid; San Pablo-CEU (n = 22), and Barcelona; UAB (n = 36). They responded, prior to their undergraduate medical course in psychiatry, to the AMI questionnaire to measure the attitudes towards mental illness and to Balon's adapted questionnaire to investigate their view towards psychiatry. The students (93.4 %) had a positive attitude towards mental illness (AMI). Attitudes towards psychiatry were fairly positive with a few negative views, specifically regarding the role of psychiatrists (items 11 and 13) and the prestige of the specialty (item 16). There were some statistically significant differences between the three medical schools in the perception of psychiatry as a medical discipline. A better attitude towards mental illness was associated with a better view of the overall merits of psychiatry. Findings suggest that Spanish medical students do not have a negative attitude towards mental illness and they have a good perception of psychiatry, although there are still some misconceptions about this specialty. These student's attitudes could favor an appropriate management of patients suffering from mental illness.

  5. A genome-wide association study of neuroticism in a population-based sample.

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    Federico C F Calboli

    Full Text Available Neuroticism is a moderately heritable personality trait considered to be a risk factor for developing major depression, anxiety disorders and dementia. We performed a genome-wide association study in 2,235 participants drawn from a population-based study of neuroticism, making this the largest association study for neuroticism to date. Neuroticism was measured by the Eysenck Personality Questionnaire. After Quality Control, we analysed 430,000 autosomal SNPs together with an additional 1.2 million SNPs imputed with high quality from the Hap Map CEU samples. We found a very small effect of population stratification, corrected using one principal component, and some cryptic kinship that required no correction. NKAIN2 showed suggestive evidence of association with neuroticism as a main effect (p < 10(-6 and GPC6 showed suggestive evidence for interaction with age (p approximately = 10(-7. We found support for one previously-reported association (PDE4D, but failed to replicate other recent reports. These results suggest common SNP variation does not strongly influence neuroticism. Our study was powered to detect almost all SNPs explaining at least 2% of heritability, and so our results effectively exclude the existence of loci having a major effect on neuroticism.

  6. Chromosome Map of Xanthomonas campestris pv. campestris 17 with Locations of Genes Involved in Xanthan Gum Synthesis and Yellow Pigmentation

    Science.gov (United States)

    Tseng, Yi-Hsiung; Choy, Ka-Tim; Hung, Chih-Hsin; Lin, Nien-Tsung; Liu, Jane-Yu; Lou, Chih-Hong; Yang, Bih-Ying; Wen, Fu-Shyan; Weng, Shu-Fen; Wu, Jung-Rung

    1999-01-01

    No plasmid was detected in Xanthomonas campestris pv. campestris 17, a strain of the causative agent of black rot in cruciferous plants isolated in Taiwan. Its chromosome was cut by PacI, PmeI, and SwaI into five, two, and six fragments, respectively, and a size of 4.8 Mb was estimated by summing the fragment lengths in these digests. Based on the data obtained from partial digestion and Southern hybridization using probes common to pairs of the overlapping fragments or prepared from linking fragments, a circular physical map bearing the PacI, PmeI, and SwaI sites was constructed for the X. campestris pv. campestris 17 chromosome. Locations of eight eps loci involved in exopolysaccharide (xanthan gum) synthesis, two rrn operons each possessing an unique I-CeuI site, one pig cluster required for yellow pigmentation, and nine auxotrophic markers were determined, using mutants isolated by mutagenesis with Tn5(pfm)CmKm. This transposon contains a polylinker with sites for several rare-cutting restriction endonucleases located between the chloramphenicol resistance and kanamycin resistance (Kmr) genes, which upon insertion introduced additional sites into the chromosome. The recA and tdh genes, with known sequences, were mapped by tagging with the polylinker-Kmr segment from Tn5(pfm)CmKm. This is the first map for X. campestris and would be useful for genetic studies of this and related Xanthomonas species. PMID:9864320

  7. Vascular endothelial growth factor (VEGFA gene variation in polycystic ovary syndrome in a Tunisian women population

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    Assila Ben Salem

    2016-10-01

    .03; OR 95 % CI = 2.05 [1.07–3.90] and a trend for correlation of the pair of haplotypes H2/H2 with prolactin levels in plasma (P = 0.077; 193.5 ± 94.3 vs 45.7 ± 7.2. These data are consistent with literature and highlight one more time the role of vascularization in the pathogeny of PCOS. Conclusions LD pattern in VEGF locus showed a similar LD pattern between the Tunisian population and the CEU. More haplotypes in the Tunisian population than in CEU was observed (22 haplotypes vs 16 haplotypes suggesting higher recombination rate in Tunisians. The study showed that there was any advantage of using haplotypes compared with SNPs taken alone.

  8. Identificação de Campylobacter jejuni e Campylobacter coli isoladas de carcaças resfriadas de Frango pela Multiplex PCR | Identification of Campylobacter jejuni and Campylobacter coli from refrigerated cicken carcasses by Multiplex PCR

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    Valeria de Mello Medeiros

    2015-08-01

    Full Text Available Campylobacter jejuni e C. coli constituem as espécies termofílicas mais frequentemente isoladas em casos de enterites humanas, devido à ingestão de alimentos à base de frango mal cozido ou através da contaminação cruzada durante a manipulação de alimentos crus. A diferenciação bioquímica de C. jejuni e C. coli apresenta inconsistências, como a hidrólise do hipurato e a sensibilidade ao ácido nalidíxico e à cefalotina. Desta forma, a aplicação de métodos moleculares se faz necessário na identificação dessas espécies. O objetivo deste trabalho foi realizar a identificação de C. jejuni e C. coli isolados de carcaças resfriadas de frango pela Multiplex PCR. Para isso, o gene que codifica uma subunidade de oxirredutase (160 pb e o gene de virulência ceuE (894 pb específicos para C. jejuni e C. coli, respectivamente, foram submetidos a PCR, simultaneamente. Dos 21 isolados analisados pela bioquímica, 19 (90,48% foram identificados como C. jejuni um (4,76% como C. coli e um (4,76% não identificado. A Multiplex PCR confirmou a presença de 90,48% de C. jejuni e 9,52% de C. coli. A abordagem proposta apresentou rapidez, sensibilidade e especificidade e, assim, poderia ser considerada uma boa alternativa para ensaios clínicos de rotina e estudos epidemiológicos. --------------------------------------------------------------------------------------------- Campylobacter jejuni and C. coli are the most frequently identified thermophilic species in cases of human enteritis caused by the consumption of food products made with undercooked chicken or cross-contamination during handling of raw food products. The biochemical differentiation of C. jejuni and C. coli shows inconsistencies, such as hippurate hydrolysis and sensitivity to nalidixic acid and cephalothin. Consequently, the use of molecular methods is necessary to identify these species. The objective of this study was to identify C. jejuni and C. coli isolates from

  9. Actitud de los alumnos de quinto de medicina hacia la salud mental Medicine 5th course student's attitudes towards mental health

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    Inmaculada Gilaberte

    2012-12-01

    Full Text Available Introducción. El estigma social que persiste hacia la enfermedad mental puede estar presente en los estudiantes de medicina e impactar en la forma de considerar ésta y la psiquiatría como especialidad. Objetivo. Investigar la actitud hacia la enfermedad mental y la psiquiatría en una muestra de estudiantes. Sujetos y métodos. Se realizó una encuesta estructurada, voluntaria y anónima a los 27 estudiantes de quinto curso del grado de medicina de la Universidad San Pablo-CEU de Madrid. La encuesta incluía un cuestionario sobre el nivel de familiaridad hacia la enfermedad mental, el cuestionario de actitud hacia la enfermedad mental (AMI y el cuestionario adaptado de Balon sobre la actitud hacia la psiquiatría. Resultados. Un total de 22 alumnos respondió la encuesta (72,7% mujeres. Estos alumnos presentaron una alta familiaridad con la enfermedad mental (puntuación media: 7,27; máximo: 11. El 81,8% refirieron una actitud positiva hacia la enfermedad mental (escala AMI. Las puntuaciones de la escala de Balon también mostraron un nivel alto de acuerdo en los méritos globales de la psiquiatría y en la eficacia de ésta. Tampoco las respuestas respecto al rol y función del psiquiatra y a la carrera profesional revelaron una postura negativa. No hubo relación entre la familiaridad y la actitud hacia la enfermedad mental o hacia la psiquiatría. Conclusiones. En esta muestra no se evidencia la existencia de estigma hacia la enfermedad mental o hacia la psiquiatría. Posteriores estudios en muestras más amplias y evaluaciones de la enseñanza de la psiquiatría son necesarios para profundizar en este tema.Introduction. Medicine students could share that stigmatizing attitudes towards mental illness and Psychiatry as specialty. Aim. To investigate the attitudes of medical students towards the mental disease and the psychiatry. Subjects and methods. 27 medicine 5th course students from San Pablo-CEU University were interviewed with a

  10. Characterization of genes encoding for acquired bacitracin resistance in Clostridium perfringens.

    Science.gov (United States)

    Charlebois, Audrey; Jalbert, Louis-Alexandre; Harel, Josée; Masson, Luke; Archambault, Marie

    2012-01-01

    Phenotypic bacitracin resistance has been reported in Clostridium perfringens. However, the genes responsible for the resistance have not yet been characterized. Ninety-nine C. perfringens isolates recovered from broilers and turkeys were tested for phenotypic bacitracin resistance. Bacitracin MIC(90) (>256 µg/ml) was identical for both turkey and chicken isolates; whereas MIC(50) was higher in turkey isolates (6 µg/ml) than in chicken isolates (3 µg/ml). Twenty-four of the 99 isolates showed high-level bacitracin resistance (MIC breakpoint >256 µg/ml) and the genes encoding for this resistance were characterized in C. perfringens c1261_A strain using primer walking. Sequence analysis and percentages of amino acid identity revealed putative genes encoding for both an ABC transporter and an overproduced undecaprenol kinase in C. perfringens c1261_A strain. These two mechanisms were shown to be both encoded by the putative bcrABD operon under the control of a regulatory gene, bcrR. Efflux pump inhibitor thioridazine was shown to increase significantly the susceptibility of strain c1261_A to bacitracin. Upstream and downstream from the bcr cluster was an IS1216-like element, which may play a role in the dissemination of this resistance determinant. Pulsed-field gel electrophoresis with prior double digestion with I-CeuI/MluI enzymes followed by hybridization analyses revealed that the bacitracin resistance genes bcrABDR were located on the chromosome. Semi-quantitative RT-PCR demonstrated that this gene cluster is expressed under bacitracin stress. Microarray analysis revealed the presence of these genes in all bacitracin resistant strains. This study reports the discovery of genes encoding for a putative ABC transporter and an overproduced undecaprenol kinase associated with high-level bacitracin resistance in C. perfringens isolates from turkeys and broiler chickens.

  11. Characterization of genes encoding for acquired bacitracin resistance in Clostridium perfringens.

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    Audrey Charlebois

    Full Text Available Phenotypic bacitracin resistance has been reported in Clostridium perfringens. However, the genes responsible for the resistance have not yet been characterized. Ninety-nine C. perfringens isolates recovered from broilers and turkeys were tested for phenotypic bacitracin resistance. Bacitracin MIC(90 (>256 µg/ml was identical for both turkey and chicken isolates; whereas MIC(50 was higher in turkey isolates (6 µg/ml than in chicken isolates (3 µg/ml. Twenty-four of the 99 isolates showed high-level bacitracin resistance (MIC breakpoint >256 µg/ml and the genes encoding for this resistance were characterized in C. perfringens c1261_A strain using primer walking. Sequence analysis and percentages of amino acid identity revealed putative genes encoding for both an ABC transporter and an overproduced undecaprenol kinase in C. perfringens c1261_A strain. These two mechanisms were shown to be both encoded by the putative bcrABD operon under the control of a regulatory gene, bcrR. Efflux pump inhibitor thioridazine was shown to increase significantly the susceptibility of strain c1261_A to bacitracin. Upstream and downstream from the bcr cluster was an IS1216-like element, which may play a role in the dissemination of this resistance determinant. Pulsed-field gel electrophoresis with prior double digestion with I-CeuI/MluI enzymes followed by hybridization analyses revealed that the bacitracin resistance genes bcrABDR were located on the chromosome. Semi-quantitative RT-PCR demonstrated that this gene cluster is expressed under bacitracin stress. Microarray analysis revealed the presence of these genes in all bacitracin resistant strains. This study reports the discovery of genes encoding for a putative ABC transporter and an overproduced undecaprenol kinase associated with high-level bacitracin resistance in C. perfringens isolates from turkeys and broiler chickens.

  12. Phenotypic Characters and Molecular Epidemiology of Campylobacter Jejuni in East China.

    Science.gov (United States)

    Zeng, Dexin; Zhang, Xiaoping; Xue, Feng; Wang, Yanhong; Jiang, Luyan; Jiang, Yuan

    2016-01-01

    In this study, we investigated the distribution, phenotypic and molecular typing characters of Campylobacter jejuni in domestic fowl, and livestock populations in East China, to provide some reference for researches on its molecular epidemiology. A total of 1250 samples were collected from different animal sources, and C. jejuni strains were then isolated and tested for antibiotic sensitivity. Antibiotics-resistance gene and pathogenic genes were detected by polymerase chain reaction. Phylogenic analysis on the C. jejuni strains was performed by multilocus sequence typing (MLST) method. The results showed that 108 out of the 1250 samples (mean 8.64%) were C. jejuni positive. These 108 C. jejuni strains were highly sensitive to antibiotics such as chloramphenicol, amoxicillin, amikacin, cefotaxime, and azithromycin, whereas they were highly resistant to antibiotics such as cefoperazone, cotrimoxazole, cefamandole, sulfamethoxazole, and cefradine. Pathogenicity related gene identification indicated that the mean carrying rate of adhesion related gene cadF and racR, flagellin gene flaA, toxin regulating gene cdtA, cdtB, cdtC, wlaN and virB11, heat shock proteins and transferring proteins related genes dnaJ and ceuE, CiaB and pldA were 92.45%, 38.69%, 73.58%, 71.70%, 52.83%, 96.23%, 12.26%, 1.89%, 0.94%, 65.09%, 39.62% and 9.43%, respectively. A total of 58.82% of these strains contained more than 6 pathogenicity-related genes. MLST typed 58 ST types from the 108 isolated C. jejuni strains, including 24 new types, and ST-21 was the major type, accounting for 39.3% of the total strains. © 2015 Institute of Food Technologists®

  13. Radioelement distributions in the Proterozoic granites and associated pegmatites of Gabal El Fereyid area, Southeastern Desert, Egypt.

    Science.gov (United States)

    Abd El-Naby, H H; Saleh, G M

    2003-10-01

    Lithologically, the rock types in the Gabal El Fereyid area are dominantly granites with minor amounts of pegmatites. The granites range in composition from tonalite to granite-adamallite with minor acidic dikes, quartz and pegmatite veins. The granite-adamallite is peraluminous and formed as a result of partial melting of amphibole-bearing rocks at depths of approximately 24-30 km and at temperatures of 800-950 degrees C. Among the different rock types, the muscovite-rich pegmatites had the highest U and Th contents (66 and 38 ppm on average, respectively). The high level of radioactivity in pegmatites is attributed to the presence of the radioactive minerals thorianite, uranophane and allanite as confirmed by XRD analysis. Binary relations of Zr/U, Zr/Th, Ce/U and Ce/Th against either U or Th in the granite-adamellite exhibit significant negative correlations indicating that both elements are not preferentially hosted in the accessory minerals phases such as zircon and monazite, but could be associated with major forming minerals such as biotite, muscovite, plagioclase and quartz, or U is situated within labile sites within granite. The uranium and thorium enrichment in the pegmatites is a two-stage process. The primary stage is magmatic whereas the secondary enrichment is from hydrothermal concentration. The magmatic U and Th are indicated by the presence of thorianite and allanite, whereas evidence of hydrothermal mineralization is the alteration of rock-forming minerals such as feldspar and the formation of secondary minerals such as uranophane and pyrite.

  14. Genotype Imputation for Latinos Using the HapMap and 1000 Genomes Project Reference Panels

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    Xiaoyi eGao

    2012-06-01

    Full Text Available Genotype imputation is a vital tool in genome-wide association studies (GWAS and meta-analyses of multiple GWAS results. Imputation enables researchers to increase genomic coverage and to pool data generated using different genotyping platforms. HapMap samples are often employed as the reference panel. More recently, the 1000 Genomes Project resource is becoming the primary source for reference panels. Multiple GWAS and meta-analyses are targeting Latinos, the most populous and fastest growing minority group in the US. However, genotype imputation resources for Latinos are rather limited compared to individuals of European ancestry at present, largely because of the lack of good reference data. One choice of reference panel for Latinos is one derived from the population of Mexican individuals in Los Angeles contained in the HapMap Phase 3 project and the 1000 Genomes Project. However, a detailed evaluation of the quality of the imputed genotypes derived from the public reference panels has not yet been reported. Using simulation studies, the Illumina OmniExpress GWAS data from the Los Angles Latino Eye Study and the MACH software package, we evaluated the accuracy of genotype imputation in Latinos. Our results show that the 1000 Genomes Project AMR+CEU+YRI reference panel provides the highest imputation accuracy for Latinos, and that also including Asian samples in the panel can reduce imputation accuracy. We also provide the imputation accuracy for each autosomal chromosome using the 1000 Genomes Project panel for Latinos. Our results serve as a guide to future imputation-based analysis in Latinos.

  15. New Software for the Fast Estimation of Population Recombination Rates (FastEPRR in the Genomic Era

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    Feng Gao

    2016-06-01

    Full Text Available Genetic recombination is a very important evolutionary mechanism that mixes parental haplotypes and produces new raw material for organismal evolution. As a result, information on recombination rates is critical for biological research. In this paper, we introduce a new extremely fast open-source software package (FastEPRR that uses machine learning to estimate recombination rate ρ (=4Ner from intraspecific DNA polymorphism data. When ρ>10 and the number of sampled diploid individuals is large enough (≥50, the variance of ρFastEPRR remains slightly smaller than that of ρLDhat. The new estimate ρcomb (calculated by averaging ρFastEPRR and ρLDhat has the smallest variance of all cases. When estimating ρFastEPRR, the finite-site model was employed to analyze cases with a high rate of recurrent mutations, and an additional method is proposed to consider the effect of variable recombination rates within windows. Simulations encompassing a wide range of parameters demonstrate that different evolutionary factors, such as demography and selection, may not increase the false positive rate of recombination hotspots. Overall, accuracy of FastEPRR is similar to the well-known method, LDhat, but requires far less computation time. Genetic maps for each human population (YRI, CEU, and CHB extracted from the 1000 Genomes OMNI data set were obtained in less than 3 d using just a single CPU core. The Pearson Pairwise correlation coefficient between the ρFastEPRR and ρLDhat maps is very high, ranging between 0.929 and 0.987 at a 5-Mb scale. Considering that sample sizes for these kinds of data are increasing dramatically with advances in next-generation sequencing technologies, FastEPRR (freely available at http://www.picb.ac.cn/evolgen/ is expected to become a widely used tool for establishing genetic maps and studying recombination hotspots in the population genomic era.

  16. New Software for the Fast Estimation of Population Recombination Rates (FastEPRR) in the Genomic Era.

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    Gao, Feng; Ming, Chen; Hu, Wangjie; Li, Haipeng

    2016-06-01

    Genetic recombination is a very important evolutionary mechanism that mixes parental haplotypes and produces new raw material for organismal evolution. As a result, information on recombination rates is critical for biological research. In this paper, we introduce a new extremely fast open-source software package (FastEPRR) that uses machine learning to estimate recombination rate [Formula: see text] (=[Formula: see text]) from intraspecific DNA polymorphism data. When [Formula: see text] and the number of sampled diploid individuals is large enough ([Formula: see text]), the variance of [Formula: see text] remains slightly smaller than that of [Formula: see text] The new estimate [Formula: see text] (calculated by averaging [Formula: see text] and [Formula: see text]) has the smallest variance of all cases. When estimating [Formula: see text], the finite-site model was employed to analyze cases with a high rate of recurrent mutations, and an additional method is proposed to consider the effect of variable recombination rates within windows. Simulations encompassing a wide range of parameters demonstrate that different evolutionary factors, such as demography and selection, may not increase the false positive rate of recombination hotspots. Overall, accuracy of FastEPRR is similar to the well-known method, LDhat, but requires far less computation time. Genetic maps for each human population (YRI, CEU, and CHB) extracted from the 1000 Genomes OMNI data set were obtained in less than 3 d using just a single CPU core. The Pearson Pairwise correlation coefficient between the [Formula: see text] and [Formula: see text] maps is very high, ranging between 0.929 and 0.987 at a 5-Mb scale. Considering that sample sizes for these kinds of data are increasing dramatically with advances in next-generation sequencing technologies, FastEPRR (freely available at http://www.picb.ac.cn/evolgen/) is expected to become a widely used tool for establishing genetic maps and studying

  17. Influence of leukotriene gene polymorphisms on chronic rhinosinusitis

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    Duval Melanie

    2008-03-01

    Full Text Available Abstract Background Chronic rhinosinusitis (CRS is increasingly viewed as an inflammatory condition of the sinonasal mucosa interacting with bacteria and/or fungi. However, factors conferring susceptibility to disease remain unknown. Advances in genomics offer powerful tools to explore this disorder. The goal of this study was to evaluate the effect of single nucleotide polymorphisms (SNP on CRS in a panel of genes related to cysteinyl leukotriene metabolism. Methods Severe cases of CRS and postal code match controls were recruited prospectively. A total of 206 cases and 200 controls were available for the present study. Using a candidate gene approach, five genes related to cysteinyl leukotriene metabolism were assessed. For each gene, we selected the maximally informative set of common SNPs (tagSNPs using the European-derived (CEU HapMap dataset. These SNPs are in arachidonate 5-lipoxygenase (ALOX5, arachidonate 5-lipoxygenase-activating protein (ALOX5AP, leukotriene C4 synthase (LTC4S, cysteinyl leukotriene receptor 1 (CYSLTR1 and cysteinyl leukotriene receptor 2 (CYSLTR2 genes. Results A total of 59 SNPs were genotyped to capture the common genetic variations within these genes. Three SNPs located within the ALOX5, CYSLTR1 and ALOX5AP genes reached the nominal p-value threshold (p Conclusion While these initial results do not support that polymorphsims in genes assessed involved in the leukotriene pathways are contributing to the pathogenesis of CRS, this initial study was not powered to detect polymorphisms with relative risk of 2.0 or less, where we could expect many gene effects for complex diseases to occur. Thus, despite this lack of significant association noted in this study, we believe that validation with external populations and the use of better-powered studies in the future may allow more conclusive findings.

  18. Worldwide distribution of the MYH9 kidney disease susceptibility alleles and haplotypes: evidence of historical selection in Africa.

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    Taras K Oleksyk

    2010-07-01

    Full Text Available MYH9 was recently identified as renal susceptibility gene (OR 3-8, p or = 60% than in European Americans (< 4%, revealing a genetic basis for a major health disparity. The population distributions of MYH9 risk alleles and the E-1 risk haplotype and the demographic and selective forces acting on the MYH9 region are not well explored. We reconstructed MYH9 haplotypes from 4 tagging single nucleotide polymorphisms (SNPs spanning introns 12-23 using available data from HapMap Phase II, and by genotyping 938 DNAs from the Human Genome Diversity Panel (HGDP. The E-1 risk haplotype followed a cline, being most frequent within sub-Saharan African populations (range 50-80%, less frequent in populations from the Middle East (9-27% and Europe (0-9%, and rare or absent in Asia, the Americas, and Oceania. The fixation indexes (F(ST for pairwise comparisons between the risk haplotypes for continental populations were calculated for MYH9 haplotypes; F(ST ranged from 0.27-0.40 for Africa compared to other continental populations, possibly due to selection. Uniquely in Africa, the Yoruba population showed high frequency extended haplotype length around the core risk allele (C compared to the alternative allele (T at the same locus (rs4821481, iHs = 2.67, as well as high population differentiation (F(ST(CEU vs. YRI = 0.51 in HapMap Phase II data, also observable only in the Yoruba population from HGDP (F(ST = 0.49, pointing to an instance of recent selection in the genomic region. The population-specific divergence in MYH9 risk allele frequencies among the world's populations may prove important in risk assessment and public health policies to mitigate the burden of kidney disease in vulnerable populations.

  19. Signatures of natural selection at the FTO (fat mass and obesity associated locus in human populations.

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    Xuanshi Liu

    Full Text Available Polymorphisms in the first intron of FTO have been robustly replicated for associations with obesity. In the Sorbs, a Slavic population resident in Germany, the strongest effect on body mass index (BMI was found for a variant in the third intron of FTO (rs17818902. Since this may indicate population specific effects of FTO variants, we initiated studies testing FTO for signatures of selection in vertebrate species and human populations.First, we analyzed the coding region of 35 vertebrate FTO orthologs with Phylogenetic Analysis by Maximum Likelihood (PAML, ω = dN/dS to screen for signatures of selection among species. Second, we investigated human population (Europeans/CEU, Yoruba/YRI, Chinese/CHB, Japanese/JPT, Sorbs SNP data for footprints of selection using DnaSP version 4.5 and the Haplotter/PhaseII. Finally, using ConSite we compared transcription factor (TF binding sites at sequences harbouring FTO SNPs in intron three.PAML analyses revealed strong conservation in coding region of FTO (ω<1. Sliding-window results from population genetic analyses provided highly significant (p<0.001 signatures for balancing selection specifically in the third intron (e.g. Tajima's D in Sorbs = 2.77. We observed several alterations in TF binding sites, e.g. TCF3 binding site introduced by the rs17818902 minor allele.Population genetic analysis revealed signatures of balancing selection at the FTO locus with a prominent signal in intron three, a genomic region with strong association with BMI in the Sorbs. Our data support the hypothesis that genes associated with obesity may have been under evolutionary selective pressure.

  20. Comparison of Genetic Variants in Cancer-Related Genes between Chinese Hui and Han Populations.

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    Chaoyong Tian

    Full Text Available The Chinese Hui population, as the second largest minority ethnic group in China, may have a different genetic background from Han people because of its unique demographic history. In this study, we aimed to identify genetic differences between Han and Hui Chinese from the Ningxia region of China by comparing eighteen single nucleotide polymorphisms in cancer-related genes.DNA samples were collected from 99 Hui and 145 Han people from the Ningxia Hui Autonomous Region in China, and SNPs were detected using an improved multiplex ligase detection reaction method. Genotyping data from six 1000 Genomes Project population samples (99 Utah residents with northern and western European ancestry (CEU, 107 Toscani in Italy (TSI, 108 Yoruba in Ibadan (YRI, 61 of African ancestry in the southwestern US (ASW, 103 Han Chinese in Beijing (CHB, and 104 Japanese in Tokyo (JPT were also included in this study. Differences in the distribution of alleles among the populations were assessed using χ2 tests, and FST was used to measure the degree of population differentiation.We found that the genetic diversity of many SNPs in cancer-related genes in the Hui Chinese in Ningxia was different from that in the Han Chinese in Ningxia. For example, the allele frequencies of four SNPs (rs13361707, rs2274223, rs465498, and rs753955 showed different genetic distributions (p0.000 between the Hui and Han populations.These results suggest that some SNPs associated with cancer-related genes vary among different Chinese ethnic groups. We suggest that population differences should be carefully considered in evaluating cancer risk and prognosis as well as the efficacy of cancer therapy.

  1. Genotypic and allelic variability in CYP19A1 among populations of African and European ancestry.

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    Athena Starlard-Davenport

    Full Text Available CYP19A1 facilitates the bioconversion of estrogens from androgens. CYP19A1 intron single nucleotide polymorphisms (SNPs may alter mRNA splicing, resulting in altered CYP19A1 activity, and potentially influencing disease susceptibility. Genetic studies of CYP19A1 SNPs have been well documented in populations of European ancestry; however, studies in populations of African ancestry are limited. In the present study, ten 'candidate' intronic SNPs in CYP19A1 from 125 African Americans (AA and 277 European Americans (EA were genotyped and their frequencies compared. Allele frequencies were also compared with HapMap and ASW 1000 Genomes populations. We observed significant differences in the minor allele frequencies between AA and EA in six of the ten SNPs including rs10459592 (p<0.0001, rs12908960 (p<0.0001, rs1902584 (p = 0.016, rs2470144 (p<0.0001, rs1961177 (p<0.0001, and rs6493497 (p = 0.003. While there were no significant differences in allele frequencies between EA and CEU in the HapMap population, a 1.2- to 19-fold difference in allele frequency for rs10459592 (p = 0.004, rs12908960 (p = 0.0006, rs1902584 (p<0.0001, rs2470144 (p = 0.0006, rs1961177 (p<0.0001, and rs6493497 (p = 0.0092 was observed between AA and the Yoruba (YRI population. Linkage disequilibrium (LD blocks and haplotype clusters that is unique to the EA population but not AA was also observed. In summary, we demonstrate that differences in the allele frequencies of CYP19A1 intron SNPs are not consistent between populations of African and European ancestry. Thus, investigations into whether CYP19A1 intron SNPs contribute to variations in cancer incidence, outcomes and pharmacological response seen in populations of different ancestry may prove beneficial.

  2. Effective selection of informative SNPs and classification on the HapMap genotype data

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    Wang Lipo

    2007-12-01

    Full Text Available Abstract Background Since the single nucleotide polymorphisms (SNPs are genetic variations which determine the difference between any two unrelated individuals, the SNPs can be used to identify the correct source population of an individual. For efficient population identification with the HapMap genotype data, as few informative SNPs as possible are required from the original 4 million SNPs. Recently, Park et al. (2006 adopted the nearest shrunken centroid method to classify the three populations, i.e., Utah residents with ancestry from Northern and Western Europe (CEU, Yoruba in Ibadan, Nigeria in West Africa (YRI, and Han Chinese in Beijing together with Japanese in Tokyo (CHB+JPT, from which 100,736 SNPs were obtained and the top 82 SNPs could completely classify the three populations. Results In this paper, we propose to first rank each feature (SNP using a ranking measure, i.e., a modified t-test or F-statistics. Then from the ranking list, we form different feature subsets by sequentially choosing different numbers of features (e.g., 1, 2, 3, ..., 100. with top ranking values, train and test them by a classifier, e.g., the support vector machine (SVM, thereby finding one subset which has the highest classification accuracy. Compared to the classification method of Park et al., we obtain a better result, i.e., good classification of the 3 populations using on average 64 SNPs. Conclusion Experimental results show that the both of the modified t-test and F-statistics method are very effective in ranking SNPs about their classification capabilities. Combined with the SVM classifier, a desirable feature subset (with the minimum size and most informativeness can be quickly found in the greedy manner after ranking all SNPs. Our method is able to identify a very small number of important SNPs that can determine the populations of individuals.

  3. Large scale association analysis identifies three susceptibility loci for coronary artery disease.

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    Stephanie Saade

    Full Text Available Genome wide association studies (GWAS and their replications that have associated DNA variants with myocardial infarction (MI and/or coronary artery disease (CAD are predominantly based on populations of European or Eastern Asian descent. Replication of the most significantly associated polymorphisms in multiple populations with distinctive genetic backgrounds and lifestyles is crucial to the understanding of the pathophysiology of a multifactorial disease like CAD. We have used our Lebanese cohort to perform a replication study of nine previously identified CAD/MI susceptibility loci (LTA, CDKN2A-CDKN2B, CELSR2-PSRC1-SORT1, CXCL12, MTHFD1L, WDR12, PCSK9, SH2B3, and SLC22A3, and 88 genes in related phenotypes. The study was conducted on 2,002 patients with detailed demographic, clinical characteristics, and cardiac catheterization results. One marker, rs6922269, in MTHFD1L was significantly protective against MI (OR=0.68, p=0.0035, while the variant rs4977574 in CDKN2A-CDKN2B was significantly associated with MI (OR=1.33, p=0.0086. Associations were detected after adjustment for family history of CAD, gender, hypertension, hyperlipidemia, diabetes, and smoking. The parallel study of 88 previously published genes in related phenotypes encompassed 20,225 markers, three quarters of which with imputed genotypes The study was based on our genome-wide genotype data set, with imputation across the whole genome to HapMap II release 22 using HapMap CEU population as a reference. Analysis was conducted on both the genotyped and imputed variants in the 88 regions covering selected genes. This approach replicated HNRNPA3P1-CXCL12 association with CAD and identified new significant associations of CDKAL1, ST6GAL1, and PTPRD with CAD. Our study provides evidence for the importance of the multifactorial aspect of CAD/MI and describes genes predisposing to their etiology.

  4. Immediate effect of ultrasound and ischemic compression techniques for the treatment of trapezius latent myofascial trigger points in healthy subjects: a randomized controlled study.

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    Aguilera, F Javier Montañez; Martín, Daniel Pecos; Masanet, Rosana Arnau; Botella, Ana Camps; Soler, Lorena Borja; Morell, Francisco Bosch

    2009-09-01

    The purpose of this study was to determine immediate effects of ischemic compression (IC) and ultrasound (US) for the treatment of myofascial trigger points (MTrPs) in the trapezius muscle. Sixty-six volunteers, all CEU-Cardenal Herrera University, Valencia, Spain, personnel, participated in this study. Subjects were healthy individuals, diagnosed with latent MTrPs in the trapezius muscle. Subjects were randomly placed into 3 groups: G1, which received IC treatment for MTrPs; G2, which received US; and G3 (control), which received sham US. The following data were recorded before and after each treatment: active range of motion (AROM) of cervical rachis measured with a cervical range of motion instrument, basal electrical activity (BEA) of muscle trapezius measured with surface electromyography, and pressure tolerance of MTrP measured with visual analogue scale assessing local pain evoked by the application of 2.5 kg/cm(2) of pressure using a pressure analog algometer. The results showed an immediate decrease in BEA of the trapezius muscle and a reduction of MTrP sensitivity after treatment with both therapeutic modalities. In the case of IC, an improvement of AROM of cervical rachis was also been obtained. In this group of participants, both treatments were shown to have an immediate effect on latent MTrPs. The results show a relation among AROM of cervical rachis, BEA of the trapezius muscle, and MTrP sensitivity of the trapezius muscle gaining short-term positive effects with use of IC.

  5. Actitud de los alumnos de quinto de medicina hacia la salud mental

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    Inmaculada Gilaberte

    Full Text Available Introducción. El estigma social que persiste hacia la enfermedad mental puede estar presente en los estudiantes de medicina e impactar en la forma de considerar ésta y la psiquiatría como especialidad. Objetivo. Investigar la actitud hacia la enfermedad mental y la psiquiatría en una muestra de estudiantes. Sujetos y métodos. Se realizó una encuesta estructurada, voluntaria y anónima a los 27 estudiantes de quinto curso del grado de medicina de la Universidad San Pablo-CEU de Madrid. La encuesta incluía un cuestionario sobre el nivel de familiaridad hacia la enfermedad mental, el cuestionario de actitud hacia la enfermedad mental (AMI y el cuestionario adaptado de Balon sobre la actitud hacia la psiquiatría. Resultados. Un total de 22 alumnos respondió la encuesta (72,7% mujeres. Estos alumnos presentaron una alta familiaridad con la enfermedad mental (puntuación media: 7,27; máximo: 11. El 81,8% refirieron una actitud positiva hacia la enfermedad mental (escala AMI. Las puntuaciones de la escala de Balon también mostraron un nivel alto de acuerdo en los méritos globales de la psiquiatría y en la eficacia de ésta. Tampoco las respuestas respecto al rol y función del psiquiatra y a la carrera profesional revelaron una postura negativa. No hubo relación entre la familiaridad y la actitud hacia la enfermedad mental o hacia la psiquiatría. Conclusiones. En esta muestra no se evidencia la existencia de estigma hacia la enfermedad mental o hacia la psiquiatría. Posteriores estudios en muestras más amplias y evaluaciones de la enseñanza de la psiquiatría son necesarios para profundizar en este tema.

  6. ANÁLISE DO PERFIL CLÍNICO DOS PACIENTES ATENDIDOS NA ESPECIALIDADE DE FISIOTERAPIA DERMATOFUNCIONAL NA CLÍNICA ESCOLA DE UMA INSTITUIÇÃO DE ENSINO SUPERIOR

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    Jhenifer Santiago de Oliveira

    2016-06-01

    Full Text Available Objective: establish the clinical profile of patients seen at a clinic physical therapy school in dermatofuncional physical therapy specialty. Methods: descriptive study, carried out by patient charts analysis met from February 2013 to November 2014, at the Clinic UniFisio School (CEU in the city of Anapolis, Goias. Results: 48 records were analyzed, these 72 92 %% female and 25% male. 16.67% have fibroedemagelóide, leprosy 20.83%, 8.33% had burns, 10.42% related to wrinkles, 10.42% had stretch marks, cancer 8.33%, 14.58% and 10 lymphedema, 42% other isolated cases. The main complaints were described pain with 24%, then 22% the loss of mobility after scar with 15%, cellulite and stretch marks with 11%, 9% decrease in amplitude of the upper limbs and 8% of the marks of expression. In analysis of physical activity frequency 58.33% of the charts analyzed patients were sedentary, 16.67% regularly practice some form of physical activity and 25% did not declare. Regarding the diagnosis has fibroedemagelóide 16.67%, 20.83% leprosy, 8.33% had burns, 10.42% related to wrinkles, 10.42% had stretch marks, cancer 8.33%, 14.58% lymphedema and even had cases that were isolated characterized as others for a total of 10.42%. conclusions: The profile goers in most patients was between 30-40 years, with most of diagnosis of leprosy and complaining of pain.

  7. Inferring separate parental admixture components in unknown DNA samples using autosomal SNPs.

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    Crouch, Daniel J M; Weale, Michael E

    2012-12-01

    The identification of ancestral admixture proportions for human DNA samples has recently had success in forensic cases. Current methods infer admixture proportions for the target sample, but not for their parents, which provides an additional layer of information that may aid certain forensic investigations. We describe new maximum likelihood methods (LEAPFrOG and LEAPFrOG Expectation Maximisation), for inferring both an individual's admixture proportions and the admixture proportions possessed by the unobserved parents, with respect to two or more source populations, using single-nucleotide polymorphism data typed only in the target individual. This is achieved by examining the increase in heterozygosity in the offspring of parents who are from different populations or who represent different mixtures from a number of source populations. We validated the methods via simulation; combining chromosomes from different Hapmap Phase III population samples to emulate first-generation admixture. Performance was strong for individuals with mixed African/European (YRI/CEU) ancestry, but poor for mixed Japanese/Chinese (JPT/CHB) ancestry, reflecting the difficulty in distinguishing closely related source populations. A total of 11 African-American trios were used to compare the parental admixture inferred from their own genotypes against that inferred purely from their offspring genotypes. We examined the performance of 34 ancestry informative markers from a multiplex kit for ancestry inference. Simulations showed that estimates were unreliable when parents had similar admixture, suggesting more markers are needed. Our results demonstrate that ancestral backgrounds of case samples and their parents are obtainable to aid in forensic investigations, provided that high-throughput methods are adopted by the forensic community.

  8. Variation in regulator of G-protein signaling 17 gene (RGS17 is associated with multiple substance dependence diagnoses

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    Zhang Huiping

    2012-05-01

    Full Text Available Abstract Background RGS17 and RGS20 encode two members of the regulator of G-protein signaling RGS-Rz subfamily. Variation in these genes may alter their transcription and thereby influence the function of G protein-coupled receptors, including opioid receptors, and modify risk for substance dependence. Methods The association of 13 RGS17 and eight RGS20 tag single nucleotide polymorphisms (SNPs was examined with four substance dependence diagnoses (alcohol (AD, cocaine (CD, opioid (OD or marijuana (MjD] in 1,905 African Americans (AAs: 1,562 cases and 343 controls and 1,332 European Americans (EAs: 981 cases and 351 controls. Analyses were performed using both χ2 tests and logistic regression analyses that covaried sex, age, and ancestry proportion. Correlation of genotypes and mRNA expression levels was assessed by linear regression analyses. Results Seven RGS17 SNPs showed a significant association with at least one of the four dependence traits after a permutation-based correction for multiple testing (0.003≤Pempirical≤0.037. The G allele of SNP rs596359, in the RGS17 promoter region, was associated with AD, CD, OD, or MjD in both populations (0.005≤Pempirical≤0.019. This allele was also associated with significantly lower mRNA expression levels of RGS17 in YRI subjects (P = 0.002 and non-significantly lower mRNA expression levels of RGS17 in CEU subjects (P = 0.185. No RGS20 SNPs were associated with any of the four dependence traits in either population. Conclusions This study demonstrated that variation in RGS17 was associated with risk for substance dependence diagnoses in both AA and EA populations.

  9. Imported poultry meat as a source of extended-spectrum cephalosporin-resistant CMY-2-producing Salmonella Heidelberg and Salmonella Minnesota in the European Union, 2014-2015.

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    Campos, Joana; Mourão, Joana; Silveira, Leonor; Saraiva, Margarida; Correia, Cristina Belo; Maçãs, Ana Paula; Peixe, Luísa; Antunes, Patrícia

    2018-01-01

    Extended-spectrum cephalosporin (ESC)-resistant Salmonella have been described at a low level in the EU, nevertheless the increasing importation of poultry meat could be an important source of epidemic strains carrying ESC resistance genes. This study evaluated ESC resistance and its genetic platform among Salmonella isolates from poultry meat products imported into Portugal as well as clonal relatedness of the isolates. All Salmonella isolates recovered from samples of fresh meat destined for import into the EU in the scope of Portuguese official border control (2014-2015) were studied. Antibiotic susceptibility and β-lactamase production was determined by disk diffusion/microdilution. Molecular studies included detection of genes encoding acquired AmpC and extended-spectrum β-lactamases, plasmid-mediated quinolone resistance and other antibiotic resistance genes by PCR/sequencing, and clonality by MLST and XbaI-PFGE. Plasmid characterisation was assessed by conjugation assays, replicon typing (PCR-PBRT/pMLST) and hybridisation experiments (I-CeuI/S1-PFGE nuclease). Isolates belonged to Salmonella Heidelberg (n = 6; ST15/eBG26) and Salmonella Minnesota (n = 1; ST548/eBG77) and presented multidrug-resistant profiles, including to ESCs and/or fluoroquinolones. All but one carried blaCMY-2, located on two epidemic plasmids, IncA/C (ST2, n = 5) or transferable IncI1 (ST12, n = 1). Salmonella Heidelberg was associated with five PFGE types, including one similar to an American epidemic clone. This study reveals imported poultry products as a source of uncommon and/or invasive ESC-resistant Salmonella strains in the EU. The increase of clinically relevant poultry-related serotypes in Europe must be taken into account in the current monitoring of antibiotic resistance trends and in re-evaluation of food regulations. Copyright © 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

  10. ERAP1 polymorphisms and haplotypes are associated with ankylosing spondylitis susceptibility and functional severity in a Spanish population.

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    Szczypiorska, Magdalena; Sánchez, Alejandra; Bartolomé, Nerea; Arteta, David; Sanz, Jesús; Brito, Elia; Fernández, Pilar; Collantes, Eduardo; Martínez, Antonio; Tejedor, Diego; Artieda, Marta; Mulero, Juan

    2011-11-01

    The aim of this study was to assess the involvement of the endoplasmic reticulum aminopeptidase 1 (ERAP1) gene in AS susceptibility and functional severity in a Spanish population. Eight single nucleotide polymorphisms (SNPs) spanning the ERAP1 gene were genotyped by allele-specific fluorescent PCR in 300 AS Spanish patients and 300 spondylarthritis-free controls. The influence of the ERAP1 SNPs on the functional severity of AS was analysed with the BASFI corrected for disease duration. Association analyses with AS susceptibility and functional severity were performed. Significant ERAP1 single marker association with AS susceptibility was found for five SNPs, namely rs30187 (allele T: P = 0.035), rs17482078 (allele C: P = 0.030), rs2287987 (allele T: P = 0.028), rs26653 (allele C: P = 0.041) and rs10050860 (allele C: P = 0.018). Three of the associated SNPs (rs17482078, rs2287987 and rs10050860) were in strong linkage disequilibrium. After imputing genotypes with the HapMap CEU data as reference, the strongest association was with rs41135 (P = 0.0046) in the 5'-upstream region of ERAP1. In addition, the SNP rs17481856 was found to be a risk factor for functional severity in AS and a borderline trend was observed for rs27044. These results suggest that the ERAP1 gene is associated with genetic predisposition to AS and influences the functional severity of the disease in a Spanish population.

  11. GLIDERS - A web-based search engine for genome-wide linkage disequilibrium between HapMap SNPs

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    Broxholme John

    2009-10-01

    Full Text Available Abstract Background A number of tools for the examination of linkage disequilibrium (LD patterns between nearby alleles exist, but none are available for quickly and easily investigating LD at longer ranges (>500 kb. We have developed a web-based query tool (GLIDERS: Genome-wide LInkage DisEquilibrium Repository and Search engine that enables the retrieval of pairwise associations with r2 ≥ 0.3 across the human genome for any SNP genotyped within HapMap phase 2 and 3, regardless of distance between the markers. Description GLIDERS is an easy to use web tool that only requires the user to enter rs numbers of SNPs they want to retrieve genome-wide LD for (both nearby and long-range. The intuitive web interface handles both manual entry of SNP IDs as well as allowing users to upload files of SNP IDs. The user can limit the resulting inter SNP associations with easy to use menu options. These include MAF limit (5-45%, distance limits between SNPs (minimum and maximum, r2 (0.3 to 1, HapMap population sample (CEU, YRI and JPT+CHB combined and HapMap build/release. All resulting genome-wide inter-SNP associations are displayed on a single output page, which has a link to a downloadable tab delimited text file. Conclusion GLIDERS is a quick and easy way to retrieve genome-wide inter-SNP associations and to explore LD patterns for any number of SNPs of interest. GLIDERS can be useful in identifying SNPs with long-range LD. This can highlight mis-mapping or other potential association signal localisation problems.

  12. Asthma and genes encoding components of the vitamin D pathway

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    Raby Benjamin A

    2009-10-01

    Full Text Available Abstract Background Genetic variants at the vitamin D receptor (VDR locus are associated with asthma and atopy. We hypothesized that polymorphisms in other genes of the vitamin D pathway are associated with asthma or atopy. Methods Eleven candidate genes were chosen for this study, five of which code for proteins in the vitamin D metabolism pathway (CYP27A1, CYP27B1, CYP2R1, CYP24A1, GC and six that are known to be transcriptionally regulated by vitamin D (IL10, IL1RL1, CD28, CD86, IL8, SKIIP. For each gene, we selected a maximally informative set of common SNPs (tagSNPs using the European-derived (CEU HapMap dataset. A total of 87 SNPs were genotyped in a French-Canadian family sample ascertained through asthmatic probands (388 nuclear families, 1064 individuals and evaluated using the Family Based Association Test (FBAT program. We then sought to replicate the positive findings in four independent samples: two from Western Canada, one from Australia and one from the USA (CAMP. Results A number of SNPs in the IL10, CYP24A1, CYP2R1, IL1RL1 and CD86 genes were modestly associated with asthma and atopy (p IL10 and VDR genes as well as in the IL10 and IL1RL1 genes were associated with asthma (p IL10 and CYP24A1 genes were again modestly associated with asthma and atopy (p IL10 and VDR was replicated in CAMP, but not in the other populations. Conclusion A number of genes involved in the vitamin D pathway demonstrate modest levels of association with asthma and atopy. Multilocus models testing genes in the same pathway are potentially more effective to evaluate the risk of asthma, but the effects are not uniform across populations.

  13. Common genetic variation near the connexin-43 gene is associated with resting heart rate in African Americans: A genome-wide association study of 13,372 participants

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    Deo, R.; Nalls, M.A.; Avery, C.L.; Smith, J.G.; Evans, D.S.; Keller, M.F.; Butler, A.M.; Buxbaum, S.G.; Li, G.; Quibrera, P. Miguel; Smith, E.N.; Tanaka, T.; Akylbekova, E.L.; Alonso, A.; Arking, D.E.; Benjamin, E.J.; Berenson, G.S.; Bis, J.C.; Chen, L.Y.; Chen, W.; Cummings, S.R.; Ellinor, P.T.; Evans, M.K.; Ferrucci, L.; Fox, E.R.; Heckbert, S.R.; Heiss, G.; Hsueh, W.C.; Kerr, K.F.; Limacher, M.C.; Liu, Y.; Lubitz, S.A.; Magnani, J.W.; Mehra, R.; Marcus, G.M.; Murray, S.S.; Newman, A.B.; Njajou, O.; North, K.E.; Paltoo, D.N.; Psaty, B.M.; Redline, S.S.; Reiner, A.P.; Robinson, J.G.; Rotter, J.I.; Samdarshi, T.E.; Schnabel, R.B.; Schork, N.J.; Singleton, A.B.; Siscovick, D.; Soliman, E.Z.; Sotoodehnia, N.; Srinivasan, S.R.; Taylor, H.A.; Trevisan, M.; Zhang, Z.; Zonderman, A.B.; Newton-Cheh, C.; Whitsel, E.A.

    2013-01-01

    BACKGROUND Genome-wide association studies have identified several genetic loci associated with variation in resting heart rate in European and Asian populations. No study has evaluated genetic variants associated with heart rate in African Americans. OBJECTIVE To identify novel genetic variants associated with resting heart rate in African Americans. METHODS Ten cohort studies participating in the Candidate-gene Association Resource and Continental Origins and Genetic Epidemiology Network consortia performed genome-wide genotyping of single nucleotide polymorphisms (SNPs) and imputed 2,954,965 SNPs using HapMap YRI and CEU panels in 13,372 participants of African ancestry. Each study measured the RR interval (ms) from 10-second resting 12-lead electrocardiograms and estimated RR-SNP associations using covariate-adjusted linear regression. Random-effects meta-analysis was used to combine cohort-specific measures of association and identify genome-wide significant loci (P ≤ 2.5 × 10−8). RESULTS Fourteen SNPs on chromosome 6q22 exceeded the genome-wide significance threshold. The most significant association was for rs9320841 (+13 ms per minor allele; P = 4.98 × 10−15). This SNP was approximately 350 kb downstream of GJA1, a locus previously identified as harboring SNPs associated with heart rate in Europeans. Adjustment for rs9320841 also attenuated the association between the remaining 13 SNPs in this region and heart rate. In addition, SNPs in MYH6, which have been identified in European genome-wide association study, were associated with similar changes in the resting heart rate as this population of African Americans. CONCLUSIONS An intergenic region downstream of GJA1 (the gene encoding connexin 43, the major protein of the human myocardial gap junction) and an intragenic region within MYH6 are associated with variation in resting heart rate in African Americans as well as in populations of European and Asian origin. PMID:23183192

  14. Gene flow between the Korean peninsula and its neighboring countries.

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    Jongsun Jung

    Full Text Available SNP markers provide the primary data for population structure analysis. In this study, we employed whole-genome autosomal SNPs as a marker set (54,836 SNP markers and tested their possible effects on genetic ancestry using 320 subjects covering 24 regional groups including Northern (=16 and Southern (=3 Asians, Amerindians (=1, and four HapMap populations (YRI, CEU, JPT, and CHB. Additionally, we evaluated the effectiveness and robustness of 50K autosomal SNPs with various clustering methods, along with their dependencies on recombination hotspots (RH, linkage disequilibrium (LD, missing calls and regional specific markers. The RH- and LD-free multi-dimensional scaling (MDS method showed a broad picture of human migration from Africa to North-East Asia on our genome map, supporting results from previous haploid DNA studies. Of the Asian groups, the East Asian group showed greater differentiation than the Northern and Southern Asian groups with respect to Fst statistics. By extension, the analysis of monomorphic markers implied that nine out of ten historical regions in South Korea, and Tokyo in Japan, showed signs of genetic drift caused by the later settlement of East Asia (South Korea, Japan and China, while Gyeongju in South East Korea showed signs of the earliest settlement in East Asia. In the genome map, the gene flow to the Korean Peninsula from its neighboring countries indicated that some genetic signals from Northern populations such as the Siberians and Mongolians still remain in the South East and West regions, while few signals remain from the early Southern lineages.

  15. Whole genome PCR scanning reveals the syntenic genome structure of toxigenic Vibrio cholerae strains in the O1/O139 population.

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    Bo Pang

    Full Text Available Vibrio cholerae is commonly found in estuarine water systems. Toxigenic O1 and O139 V. cholerae strains have caused cholera epidemics and pandemics, whereas the nontoxigenic strains within these serogroups only occasionally lead to disease. To understand the differences in the genome and clonality between the toxigenic and nontoxigenic strains of V. cholerae serogroups O1 and O139, we employed a whole genome PCR scanning (WGPScanning method, an rrn operon-mediated fragment rearrangement analysis and comparative genomic hybridization (CGH to analyze the genome structure of different strains. WGPScanning in conjunction with CGH revealed that the genomic contents of the toxigenic strains were conservative, except for a few indels located mainly in mobile elements. Minor nucleotide variation in orthologous genes appeared to be the major difference between the toxigenic strains. rrn operon-mediated rearrangements were infrequent in El Tor toxigenic strains tested using I-CeuI digested pulsed-field gel electrophoresis (PFGE analysis and PCR analysis based on flanking sequence of rrn operons. Using these methods, we found that the genomic structures of toxigenic El Tor and O139 strains were syntenic. The nontoxigenic strains exhibited more extensive sequence variations, but toxin coregulated pilus positive (TCP+ strains had a similar structure. TCP+ nontoxigenic strains could be subdivided into multiple lineages according to the TCP type, suggesting the existence of complex intermediates in the evolution of toxigenic strains. The data indicate that toxigenic O1 El Tor and O139 strains were derived from a single lineage of intermediates from complex clones in the environment. The nontoxigenic strains with non-El Tor type TCP may yet evolve into new epidemic clones after attaining toxigenic attributes.

  16. An unusual haplotype structure on human chromosome 8p23 derived from the inversion polymorphism.

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    Deng, Libin; Zhang, Yuezheng; Kang, Jian; Liu, Tao; Zhao, Hongbin; Gao, Yang; Li, Chaohua; Pan, Hao; Tang, Xiaoli; Wang, Dunmei; Niu, Tianhua; Yang, Huanming; Zeng, Changqing

    2008-10-01

    Chromosomal inversion is an important type of genomic variations involved in both evolution and disease pathogenesis. Here, we describe the refined genetic structure of a 3.8-Mb inversion polymorphism at chromosome 8p23. Using HapMap data of 1,073 SNPs generated from 209 unrelated samples from CEPH-Utah residents with ancestry from northern and western Europe (CEU); Yoruba in Ibadan, Nigeria (YRI); and Asian (ASN) samples, which were comprised of Han Chinese from Beijing, China (CHB) and Japanese from Tokyo, Japan (JPT)-we successfully deduced the inversion orientations of all their 418 haplotypes. In particular, distinct haplotype subgroups were identified based on principal component analysis (PCA). Such genetic substructures were consistent with clustering patterns based on neighbor-joining tree reconstruction, which revealed a total of four haplotype clades across all samples. Metaphase fluorescence in situ hybridization (FISH) in a subset of 10 HapMap samples verified their inversion orientations predicted by PCA or phylogenetic tree reconstruction. Positioning of the outgroup haplotype within one of YRI clades suggested that Human NCBI Build 36-inverted order is most likely the ancestral orientation. Furthermore, the population differentiation test and the relative extended haplotype homozygosity (REHH) analysis in this region discovered multiple selection signals, also in a population-specific manner. A positive selection signal was detected at XKR6 in the ASN population. These results revealed the correlation of inversion polymorphisms to population-specific genetic structures, and various selection patterns as possible mechanisms for the maintenance of a large chromosomal rearrangement at 8p23 region during evolution. In addition, our study also showed that haplotype-based clustering methods, such as PCA, can be applied in scanning for cryptic inversion polymorphisms at a genome-wide scale.

  17. No Clear Differences between Organic or Conventional Pig Farms in the Genetic Diversity or Virulence of Campylobacter coli Isolates

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    Martine Denis

    2017-06-01

    Full Text Available To evaluate the impact of pig farm management on the genetic diversity and on the virulence of Campylobacter coli, we characterized isolates from 19 organic pig farms (62 isolates and from 24 conventional pig farms (58 isolates. The 120 C. coli isolates were typed using pulsed field gel electrophoresis (PFGE and multilocus sequence typing (MLST and the presence of nine virulence genes was screened using real-time PCR. The capacity of adhesion and invasion of 61 isolates (32 from organic and 29 from conventional farms were then tested on human intestinal Caco-2 cells. A total of 59 PFGE types and of 50 sequence types (STs were identified. Twelve PFGE types and nine STs, accounting for 34 and 41.6% of the isolates, respectively, were common between the two production systems with ST854 dominating (18.3% of the isolates. Twenty-nine PFGE types and 25 STs were only found in isolates from organic farms, and 18 PFGE types and 16 STs from conventional farms. No significant differences were found in diversity despite the differences in rearing systems, except at the locus level for the glnA, gltA, and uncA genes. All isolates, regardless of their origin, carried the ceuE, iam, ciaB, and flaA genes and more than 95% of the isolates carried the cadF and cdtABC genes. No significant differences were found in pathogenicity between the two farming systems. The pathogenicity of the C. coli isolates was low compared to C. jejuni control strains tested. The plasmid gene virb11 was detected in only 13 isolates from organic farms; these isolates showed greater invasion capacity than those without this gene. Our study indicates that pig farm management does not significantly affect the diversity and the virulence of Campylobacter coli isolated from pigs. The common genotypes between conventional and organic farms may indicate that some genotypes are adapted to pigs.

  18. No Clear Differences between Organic or Conventional Pig Farms in the Genetic Diversity or Virulence ofCampylobacter coliIsolates.

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    Denis, Martine; Nagard, Bérengère; Rose, Valérie; Bourgoin, Kévin; Cutimbo, Mélina; Kerouanton, Annaëlle

    2017-01-01

    To evaluate the impact of pig farm management on the genetic diversity and on the virulence of Campylobacter coli , we characterized isolates from 19 organic pig farms (62 isolates) and from 24 conventional pig farms (58 isolates). The 120 C. coli isolates were typed using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) and the presence of nine virulence genes was screened using real-time PCR. The capacity of adhesion and invasion of 61 isolates (32 from organic and 29 from conventional farms) were then tested on human intestinal Caco-2 cells. A total of 59 PFGE types and of 50 sequence types (STs) were identified. Twelve PFGE types and nine STs, accounting for 34 and 41.6% of the isolates, respectively, were common between the two production systems with ST854 dominating (18.3% of the isolates). Twenty-nine PFGE types and 25 STs were only found in isolates from organic farms, and 18 PFGE types and 16 STs from conventional farms. No significant differences were found in diversity despite the differences in rearing systems, except at the locus level for the glnA, gltA , and uncA genes. All isolates, regardless of their origin, carried the ceuE, iam, ciaB , and flaA genes and more than 95% of the isolates carried the cadF and cdtABC genes. No significant differences were found in pathogenicity between the two farming systems. The pathogenicity of the C. coli isolates was low compared to C. jejuni control strains tested. The plasmid gene virb11 was detected in only 13 isolates from organic farms; these isolates showed greater invasion capacity than those without this gene. Our study indicates that pig farm management does not significantly affect the diversity and the virulence of Campylobacter coli isolated from pigs. The common genotypes between conventional and organic farms may indicate that some genotypes are adapted to pigs.

  19. Alcohol and tobacco consumption affect the oral carriage of Candida albicans and mutans streptococci.

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    Sheth, C C; Makda, K; Dilmahomed, Z; González, R; Luzi, A; Jovani-Sancho, M Del M; Veses, V

    2016-10-01

    This study sought to determine if there is a relationship between the consumption of alcohol and tobacco and oral colonization by mutans streptococci and Candida species. Subjects were recruited from the University Dental Clinic of CEU Cardenal Herrera University (Moncada, Valencia). Information on alcohol and tobacco consumption was obtained by questionnaire. Individual stimulated saliva samples from 105 patients were obtained and selective media was used to isolate and quantify mutans streptococci and Candida spp. colony forming units per millilitre of saliva (CFU ml(-1) ). Samples were stratified by duration and quantity of alcohol and tobacco consumption. Alcohol consumption statistically significantly decreased oral carriage of mutans streptococci, whereas there was no effect on Candida albicans colonization levels. Tobacco users were found to harbour elevated levels of C. albicans; however, there was no observed effect on bacterial colonization by mutans streptococci. The carriage of other species investigated, such as Candida krusei, Candida tropicalis and lactobacilli, do not show a response to the consumption of the stimulants analysed. Microbial colonization of the oral cavity changes in a species-specific manner in response to dietary and social habits such as drinking alcohol and smoking. In this paper, we evaluate the effect of alcohol and tobacco consumption on key species of the oral microflora. Our results show species-specific changes in two major opportunistic pathogens, such as Candida albicans and mutans streptococci, whereas other members of oral microflora are not affected by the consumption of the stimulants studied. We believe this original paper will contribute to raise awareness among the dental community towards a more personalized oral health assessment, taking in consideration alcohol and tobacco consumption in the prevention of specific oral and systemic pathologies. © 2016 The Society for Applied Microbiology.

  20. Profile of guanfacine extended release and its potential in the treatment of attention-deficit hyperactivity disorder

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    Martinez-Raga J

    2015-05-01

    Full Text Available Jose Martinez-Raga,1,2 Carlos Knecht,3 Raquel de Alvaro4 1Teaching Unit of Psychiatry and Psychological Medicine, University Hospital Doctor Peset, University of Valencia, 2CEU Cardenal Herrera University, 3Área de Salud Mental, Hospital Padre Jofré, Valencia, 4Hospital General, Consorcio Hospitalario Provincial, Castellon, Spain Abstract: The α2-adrenergic receptor agonist guanfacine, in its extended-release formulation (GXR, is the most recent nonstimulant medication approved in several countries for the treatment of attention-deficit hyperactivity disorder (ADHD as monotherapy and as adjunctive pharmacotherapy to stimulants in children and adolescents. The present paper aims to review comprehensively and critically the pharmacodynamic and pharmacokinetic characteristics and the published evidence on the efficacy and safety profile of GXR in the treatment of ADHD. A comprehensive search of relevant databases (PubMed, Embase, and PsycInfo was conducted to identify studies published in peer-reviewed journals until January 15, 2015. Though the precise mechanism of action of guanfacine in the treatment of ADHD is not fully understood, it is thought to act directly by enhancing noradrenaline functioning via α2A-adrenoceptors in the prefrontal cortex. Weight-adjusted doses should be used, with a dosing regime on a milligram per kilogram basis, starting at doses in the range 0.05–0.08 mg/kg/day, up to 0.12 mg/kg/day. As evidenced in short-term randomized controlled trials and in long-term open-label extension studies, GXR has been shown to be effective as monotherapy in the treatment of ADHD. Furthermore, GXR has also been found to be effective as adjunctive therapy to stimulant medications in patients with suboptimal responses to stimulants. Many of the adverse reactions associated with GXR, particularly sedation-related effects, were dose-related, transient, mild to moderate in severity, and did not interfere with attention or overall

  1. The effect of inversion at 8p23 on BLK association with lupus in Caucasian population.

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    Bahram Namjou

    Full Text Available To explore the potential influence of the polymorphic 8p23.1 inversion on known autoimmune susceptibility risk at or near BLK locus, we validated a new bioinformatics method that utilizes SNP data to enable accurate, high-throughput genotyping of the 8p23.1 inversion in a Caucasian population.Principal components analysis (PCA was performed using markers inside the inversion territory followed by k-means cluster analyses on 7416 European derived and 267 HapMaP CEU and TSI samples. A logistic regression conditional analysis was performed.Three subgroups have been identified; inversion homozygous, heterozygous and non-inversion homozygous. The status of inversion was further validated using HapMap samples that had previously undergone Fluorescence in situ hybridization (FISH assays with a concordance rate of above 98%. Conditional analyses based on the status of inversion were performed. We found that overall association signals in the BLK region remain significant after controlling for inversion status. The proportion of lupus cases and controls (cases/controls in each subgroup was determined to be 0.97 for the inverted homozygous group (1067 cases and 1095 controls, 1.12 for the inverted heterozygous group (1935 cases 1717 controls and 1.36 for non-inverted subgroups (924 cases and 678 controls. After calculating the linkage disequilibrium between inversion status and lupus risk haplotype we found that the lupus risk haplotype tends to reside on non-inversion background. As a result, a new association effect between non-inversion status and lupus phenotype has been identified ((p = 8.18×10(-7, OR = 1.18, 95%CI = 1.10-1.26.Our results demonstrate that both known lupus risk haplotype and inversion status act additively in the pathogenesis of lupus. Since inversion regulates expression of many genes in its territory, altered expression of other genes might also be involved in the development of lupus.

  2. Host-associated bacterial taxa from Chlorobi, Chloroflexi, GN02, Synergistetes, SR1, TM7, and WPS-2 Phyla/candidate divisions

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    Anuj Camanocha

    2014-10-01

    Full Text Available Background and objective: In addition to the well-known phyla Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Spirochaetes, Fusobacteria, Tenericutes, and Chylamydiae, the oral microbiomes of mammals contain species from the lesser-known phyla or candidate divisions, including Synergistetes, TM7, Chlorobi, Chloroflexi, GN02, SR1, and WPS-2. The objectives of this study were to create phyla-selective 16S rDNA PCR primer pairs, create selective 16S rDNA clone libraries, identify novel oral taxa, and update canine and human oral microbiome databases. Design: 16S rRNA gene sequences for members of the lesser-known phyla were downloaded from GenBank and Greengenes databases and aligned with sequences in our RNA databases. Primers with potential phylum level selectivity were designed heuristically with the goal of producing nearly full-length 16S rDNA amplicons. The specificity of primer pairs was examined by making clone libraries from PCR amplicons and determining phyla identity by BLASTN analysis. Results: Phylum-selective primer pairs were identified that allowed construction of clone libraries with 96–100% specificity for each of the lesser-known phyla. From these clone libraries, seven human and two canine novel oral taxa were identified and added to their respective taxonomic databases. For each phylum, genome sequences closest to human oral taxa were identified and added to the Human Oral Microbiome Database to facilitate metagenomic, transcriptomic, and proteomic studies that involve tiling sequences to the most closely related taxon. While examining ribosomal operons in lesser-known phyla from single-cell genomes and metagenomes, we identified a novel rRNA operon order (23S-5S-16S in three SR1 genomes and the splitting of the 23S rRNA gene by an I-CeuI-like homing endonuclease in a WPS-2 genome. Conclusions: This study developed useful primer pairs for making phylum-selective 16S rRNA clone libraries. Phylum-specific libraries

  3. Comprehensive evaluation of imputation performance in African Americans.

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    Chanda, Pritam; Yuhki, Naoya; Li, Man; Bader, Joel S; Hartz, Alex; Boerwinkle, Eric; Kao, W H Linda; Arking, Dan E

    2012-07-01

    Imputation of genome-wide single-nucleotide polymorphism (SNP) arrays to a larger known reference panel of SNPs has become a standard and an essential part of genome-wide association studies. However, little is known about the behavior of imputation in African Americans with respect to the different imputation algorithms, the reference population(s) and the reference SNP panels used. Genome-wide SNP data (Affymetrix 6.0) from 3207 African American samples in the Atherosclerosis Risk in Communities Study (ARIC) was used to systematically evaluate imputation quality and yield. Imputation was performed with the imputation algorithms MACH, IMPUTE and BEAGLE using several combinations of three reference panels of HapMap III (ASW, YRI and CEU) and 1000 Genomes Project (pilot 1 YRI June 2010 release, EUR and AFR August 2010 and June 2011 releases) panels with SNP data on chromosomes 18, 20 and 22. About 10% of the directly genotyped SNPs from each chromosome were masked, and SNPs common between the reference panels were used for evaluating the imputation quality using two statistical metrics-concordance accuracy and Cohen's kappa (κ) coefficient. The dependencies of these metrics on the minor allele frequencies (MAF) and specific genotype categories (minor allele homozygotes, heterozygotes and major allele homozygotes) were thoroughly investigated to determine the best panel and method for imputation in African Americans. In addition, the power to detect imputed SNPs associated with simulated phenotypes was studied using the mean genotype of each masked SNP in the imputed data. Our results indicate that the genotype concordances after stratification into each genotype category and Cohen's κ coefficient are considerably better equipped to differentiate imputation performance compared with the traditionally used total concordance statistic, and both statistics improved with increasing MAF irrespective of the imputation method. We also find that both MACH and IMPUTE

  4. Congruence as a measurement of extended haplotype structure across the genome

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    Baschal Erin E

    2012-02-01

    Full Text Available Abstract Background Historically, extended haplotypes have been defined using only a few data points, such as alleles for several HLA genes in the MHC. High-density SNP data, and the increasing affordability of whole genome SNP typing, creates the opportunity to define higher resolution extended haplotypes. This drives the need for new tools that support quantification and visualization of extended haplotypes as defined by as many as 2000 SNPs. Confronted with high-density SNP data across the major histocompatibility complex (MHC for 2,300 complete families, compiled by the Type 1 Diabetes Genetics Consortium (T1DGC, we developed software for studying extended haplotypes. Methods The software, called ExHap (Extended Haplotype, uses a similarity measurement we term congruence to identify and quantify long-range allele identity. Using ExHap, we analyzed congruence in both the T1DGC data and family-phased data from the International HapMap Project. Results Congruent chromosomes from the T1DGC data have between 96.5% and 99.9% allele identity over 1,818 SNPs spanning 2.64 megabases of the MHC (HLA-DRB1 to HLA-A. Thirty-three of 132 DQ-DR-B-A defined haplotype groups have > 50% congruent chromosomes in this region. For example, 92% of chromosomes within the DR3-B8-A1 haplotype are congruent from HLA-DRB1 to HLA-A (99.8% allele identity. We also applied ExHap to all 22 autosomes for both CEU and YRI cohorts from the International HapMap Project, identifying multiple candidate extended haplotypes. Conclusions Long-range congruence is not unique to the MHC region. Patterns of allele identity on phased chromosomes provide a simple, straightforward approach to visually and quantitatively inspect complex long-range structural patterns in the genome. Such patterns aid the biologist in appreciating genetic similarities and differences across cohorts, and can lead to hypothesis generation for subsequent studies.

  5. Accuracy of CNV Detection from GWAS Data.

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    Dandan Zhang

    2011-01-01

    Full Text Available Several computer programs are available for detecting copy number variants (CNVs using genome-wide SNP arrays. We evaluated the performance of four CNV detection software suites--Birdsuite, Partek, HelixTree, and PennCNV-Affy--in the identification of both rare and common CNVs. Each program's performance was assessed in two ways. The first was its recovery rate, i.e., its ability to call 893 CNVs previously identified in eight HapMap samples by paired-end sequencing of whole-genome fosmid clones, and 51,440 CNVs identified by array Comparative Genome Hybridization (aCGH followed by validation procedures, in 90 HapMap CEU samples. The second evaluation was program performance calling rare and common CNVs in the Bipolar Genome Study (BiGS data set (1001 bipolar cases and 1033 controls, all of European ancestry as measured by the Affymetrix SNP 6.0 array. Accuracy in calling rare CNVs was assessed by positive predictive value, based on the proportion of rare CNVs validated by quantitative real-time PCR (qPCR, while accuracy in calling common CNVs was assessed by false positive/false negative rates based on qPCR validation results from a subset of common CNVs. Birdsuite recovered the highest percentages of known HapMap CNVs containing >20 markers in two reference CNV datasets. The recovery rate increased with decreased CNV frequency. In the tested rare CNV data, Birdsuite and Partek had higher positive predictive values than the other software suites. In a test of three common CNVs in the BiGS dataset, Birdsuite's call was 98.8% consistent with qPCR quantification in one CNV region, but the other two regions showed an unacceptable degree of accuracy. We found relatively poor consistency between the two "gold standards," the sequence data of Kidd et al., and aCGH data of Conrad et al. Algorithms for calling CNVs especially common ones need substantial improvement, and a "gold standard" for detection of CNVs remains to be established.

  6. Detection of Campylobacter species and Arcobacter butzleri in stool samples by use of real-time multiplex PCR.

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    de Boer, Richard F; Ott, Alewijn; Güren, Pinar; van Zanten, Evert; van Belkum, Alex; Kooistra-Smid, Anna M D

    2013-01-01

    The presence of Campylobacter (or Campylobacter-like) species in stools from patients suspected of infectious gastroenteritis (n = 493) was investigated using real-time PCR for detection of Arcobacter butzleri (hsp60 gene), Campylobacter coli (ceuE gene), Campylobacter jejuni (mapA), five acknowledged pathogenic Campylobacter spp. (C16S_Lund assay), and the Campylobacter genus (C16S_LvI assay). In total, 71.4% of the samples were positive for Campylobacter DNA (n = 352) by a Campylobacter genus-specific (C16S_LvI) assay. A total of 23 samples (4.7%) were positive in the C16S_Lund assay, used for detection of C. jejuni, C. coli, C. lari, C. upsaliensis, and C. hyointestinalis. Subsequent identification of these samples yielded detection frequencies (DF) of 4.1% (C. jejuni), 0.4% (C. coli), and 0.4% (C. upsaliensis). The DF of A. butzleri was 0.4%. Interestingly, sequencing of a subgroup (n = 46) of C16S_LvI PCR-positive samples resulted in a considerable number of Campylobacter concisus-positive samples (n = 20). PCR-positive findings with the C16S_Lund and C. jejuni/C. coli-specific assays were associated with more serious clinical symptoms (diarrhea and blood). Threshold cycle (C(T)) values of C. jejuni/C. coli PCR-positive samples were comparable to those of the C16S_Lund PCR (P = 0.21). C(T) values for both assays were significantly lower than those of the C16S_LvI assay (P < 0.001 and P < 0.00001, respectively). In conclusion, this study demonstrated that in combination, the C. jejuni/C coli-specific assays and the C16S_Lund assay are both useful for routine screening purposes. Furthermore, the DF of the emerging pathogen C. concisus was at least similar to the DF of C. jejuni.

  7. Identification of polymorphic inversions from genotypes

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    Cáceres Alejandro

    2012-02-01

    Full Text Available Abstract Background Polymorphic inversions are a source of genetic variability with a direct impact on recombination frequencies. Given the difficulty of their experimental study, computational methods have been developed to infer their existence in a large number of individuals using genome-wide data of nucleotide variation. Methods based on haplotype tagging of known inversions attempt to classify individuals as having a normal or inverted allele. Other methods that measure differences between linkage disequilibrium attempt to identify regions with inversions but unable to classify subjects accurately, an essential requirement for association studies. Results We present a novel method to both identify polymorphic inversions from genome-wide genotype data and classify individuals as containing a normal or inverted allele. Our method, a generalization of a published method for haplotype data 1, utilizes linkage between groups of SNPs to partition a set of individuals into normal and inverted subpopulations. We employ a sliding window scan to identify regions likely to have an inversion, and accumulation of evidence from neighboring SNPs is used to accurately determine the inversion status of each subject. Further, our approach detects inversions directly from genotype data, thus increasing its usability to current genome-wide association studies (GWAS. Conclusions We demonstrate the accuracy of our method to detect inversions and classify individuals on principled-simulated genotypes, produced by the evolution of an inversion event within a coalescent model 2. We applied our method to real genotype data from HapMap Phase III to characterize the inversion status of two known inversions within the regions 17q21 and 8p23 across 1184 individuals. Finally, we scan the full genomes of the European Origin (CEU and Yoruba (YRI HapMap samples. We find population-based evidence for 9 out of 15 well-established autosomic inversions, and for 52 regions

  8. Spread of bla(CTX-M-14) is driven mainly by IncK plasmids disseminated among Escherichia coli phylogroups A, B1, and D in Spain.

    Science.gov (United States)

    Valverde, Aránzazu; Cantón, Rafael; Garcillán-Barcia, M Pilar; Novais, Angela; Galán, Juan Carlos; Alvarado, Andrés; de la Cruz, Fernando; Baquero, Fernando; Coque, Teresa M

    2009-12-01

    Since its first description in 2000, CTX-M-14 has become one of the most widespread extended-spectrum beta-lactamases in Spain. In the present Escherichia coli multilevel population genetic study involving the characterization of phylogroups, clones, plasmids, and genetic platforms, 61 isolates from 16 hospitalized patients and 40 outpatients and healthy volunteers recovered from 2000 to 2005 were analyzed. Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] type, phylogenetic group, multilocus sequence type [MLST]) was established by standard methods. Analysis of transferred plasmids (I-CeuI; S1 nuclease; restriction fragment length polymorphism analysis; and analysis of RNA interference, replicase, and relaxase) was performed by PCR, sequencing, and hybridization. The genetic environment of bla(CTX-M-14) was characterized by PCR on the basis of known associated structures (ISEcp1, IS903, ISCR1). The isolates were mainly recovered from patients in the community (73.8%; 45/61) with urinary tract infections (62.2%; 28/45). They were clonally unrelated by PFGE and corresponded to phylogenetic groups A (36.1%), D (34.4%), and B1 (29.5%). MLST revealed a high degree of sequence type (ST) diversity among phylogroup D isolates and the overrepresentation of the ST10 complex among phylogroup A isolates and ST359/ST155 among phylogroup B1 isolates. Two variants of bla(CTX-M-14) previously designated bla(CTX-M-14a) (n = 59/61) and bla(CTX-M-14b) (n = 2/61) were detected. bla(CTX-M-14a) was associated with either ISEcp1 within IncK plasmids (n = 27), ISCR1 linked to an IncHI2 plasmid (n = 1), or ISCR1 linked to IncI-like plasmids (n = 3). The bla(CTX-M-14b) identified was associated with an ISCR1 element located in an IncHI2 plasmid (n = 1) or with ISEcp1 located in IncK (n = 1). The CTX-M-14-producing E. coli isolates in our geographic area are frequent causes of community-acquired urinary tract infections. The increase in the incidence of such isolates is

  9. Prevalence and antimicrobial resistance profile of Campylobacter spp. isolated from conventional and antimicrobial-free swine production systems from different U.S. regions.

    Science.gov (United States)

    Tadesse, Daniel A; Bahnson, Peter B; Funk, Julie A; Thakur, Siddhartha; Morrow, William E Morgan; Wittum, Thomas; DeGraves, Fred; Rajala-Schultz, Paivi; Gebreyes, Wondwossen A

    2011-03-01

    We conducted a study to compare the prevalence and antimicrobial resistance profile of Campylobacter isolated from 34 farm-slaughter pair cohorts of pigs raised in conventional and antimicrobial-free (ABF) production systems. Isolates originated from four different states of two geographic regions (region 1--Ohio and Michigan; region 2--Wisconsin and Iowa). A total of 838 fecal and 1173 carcass samples were examined. Campylobacter isolates were speciated using multiplex polymerase chain reaction targeting ceuE and hipO genes. The minimum inhibitory concentration was determined using agar dilution to a panel of six antimicrobials: chloramphenicol, erythromycin, gentamicin, ciprofloxacin, nalidixic acid, and tetracycline. Campylobacter spp. was isolated from 472 of 838 pigs (56.3%). Campylobacter prevalence did not vary significantly based on production system (conventional [58.9%] and ABF [53.7%], odds ratio [OR] 1.4, 95% confidence interval [CI] 0.8-2.6, p = 0.24) or geographic region (region 1 [54.1%] and region 2 [58.2%], OR 1.02, 95% CI 0.6-1.9, p = 0.92). At slaughter plant, Campylobacter prevalence varied based on processing stages (19.4% at pre-evisceration, 25.3% at postevisceration, and 3.2% at postchill). Resistance was common to tetracycline (64.5%), erythromycin (47.9%), and nalidixic acid (23.5%). Campylobacter isolates from conventional production systems were more likely to be erythromycin resistant than from ABF (OR 3.2, 95% CI 1.4-7.2, p = 0.01). The proportion of ciprofloxacin-resistant Campylobacter coli isolates were 3.7% and 1.2% from ABF and conventional production systems, respectively. Thirty-seven out of 1257 C. coli (2.9%) were resistant to both erythromycin and ciprofloxacin, drugs of choice for treatment of invasive human campylobacteriosis. The finding of ciprofloxacin resistance, particularly from ABF herds, has significant implications on the potential role of risk factors other than mere antimicrobial use for production

  10. Anemia e renda per capita familiar de crianças freqüentadoras da creche do Centro Educacional Unificado Cidade Dutra, no Município de São Paulo Anemia and per capita income in children enrolled in a childhood education center in São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Teresinha Stumpf Souto

    2007-06-01

    Full Text Available OBJETIVO: Avaliar a freqüência de anemia de acordo com a renda familiar per capita de crianças matriculadas no Centro de Educação Infantil (CEI do Centro Educacional Unificado (CEU Cidade Dutra, no Município de São Paulo, São Paulo, em 2004. MÉTODOS: Estudo transversal, com 190 crianças de 11 a 57 meses de idade distribuídas em duas faixas de renda familiar per capita ( 1 salário mínimo. Foram consideradas anêmicas as crianças com taxa de hemoglobina OBJECTIVE: To evaluate the frequency of anemia according to family per capita income in children enrolled in a day care center in São Paulo, Brazil, in 2004. METHODS: This cross-sectional study enrolled 190 children aged 11 to 57 months divided into two groups of per capita income ( 1 minimum wage. Hemoglobin level was evaluated in capillary blood (finger-stick test using a Hemocue® portable photometer. Anemia was considered when hemoglobin level was below 11g/dL. The results were analyzed using chi-square and Kruskal-Wallis tests. RESULTS: Anemia was found in 31.6% of the investigated children, with a higher proportion in younger children. The frequency of anemia was, respectively, 72.7%, 41.2%, 19.7% and 15.4% in children aged 11-23 months age, 24-35 months, 36-47 months, and 48-57 months. Among children of families with low per capita income, the frequency of anemia was 36.7%. In families earning more than one minimum wage per capita per month, there were 22.9% of children with anemia. CONCLUSIONS: At the day care center, the frequency of anemia was higher among children aged 11-23 months, especially when the family per capita income was less than one minimum wage per month.

  11. Prevalence and characterization of Campylobacter jejuni isolated from pasture flock poultry.

    Science.gov (United States)

    Hanning, Irene; Biswas, Debabrata; Herrera, Paul; Roesler, Mary; Ricke, Steven C

    2010-09-01

    The growing interest in organic and natural foods warrants a greater need for information on the food safety of these products. In this study, samples were taken from 2 pasture flock farms (N = 178; feed, water, drag swabs, and insect traps), pasture flock retail carcasses (N = 48) and 1 pasture flock processing facility (N = 16) over a period of 8 mo. A total of 105 Campylobacter isolates were obtained from 53 (30%), 36 (75%), and 16 (100%) samples from the farms, retail carcasses, and processing facility, respectively. Of the 105 isolates collected, 65 were C. jejuni, 31 were C. coli, and 9 were other Campylobacter spp. Using PCR, the C. jejuni isolates were further analyzed for virulence genes involved in colonization and survival (flaA, flaC, cadF, dnaJ, racR, cbrR), invasion (virB11, ciaB, pldA), protection against harsh conditions (sodB, htrA, clpA), toxin production (cdtA, cdtB, cdtC), siderophore transport (ceuE), and ganglioside mimicry (wlaN). In addition, the short variable region of the flaA locus (flaA SVR) was sequenced to determine the genetic diversity of the C. jejuni isolates. The flaA SVR diversity indices increased along the farm to carcass continuum. PCR-based analysis indicated a low prevalence of 5 genes involved in colonization (dnaJ, ciaB, pldA, racR, virB11). The results of this survey indicate that the prevalence of Campylobacter on organic retail carcasses is similar to prevalence reports of Campylobacter on conventional retail carcasses. However, the genetic diversity of the flaA SVR genotypes increased along the farm to carcass continuum that contrasted with conventional poultry studies. Campylobacter jejuni is a leading cause of foodborne illness with poultry and poultry products being leading sources of infection. Free-range and pasture flock chickens are becoming more popular; however, there is an inherent biosecurity risk that can increase the prevalence of foodborne pathogens in these flocks. This study aimed to determine sources

  12. El primer eslabón de las matemáticas en las facultades de CC. Económicas y Empresariales: Los análisis económicos lineales

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    María del Carmen Escribano Ródenas

    2005-01-01

    Full Text Available Los programas de la asignatura Matemáticas de primer curso de las Facultades de CC. Económicas y Empresariales, en muy pocos casos, suelen incluir un primer capítulo introductorio destinado a familiarizar a los alumnos con los principales conceptos de la ciencia económica y con la formulación matemática de los problemas económicos, incluso, si este tema aparece en la relación de contenidos, en la realidad no se imparte. En la Universidad San Pablo – CEU, la experiencia nos ha conducido a incorporar un capítulo que denominamos “Análisis Económicos Lineales”. De esta manera se introduce el concepto de relación lineal en el ámbito del análisis económico, distinguiendo así, distintos tipos de relaciones y diferentes formulaciones matemáticas apropiadas para cada una de aquellas. Teniendo en cuenta que un análisis económico cuantitativo se inicia mediante un proceso de modelación que finaliza con la formulación de un modelo matemáticoeconómico, se distinguen distintos tipos de modelos económicos lineales, poniendo especial énfasis no sólo en la formulación matemática de los modelos sino también en la utilización de las herramientas matemáticas apropiadas para su resolución. Si bien son numerosos los campos de la Economía en los que se realizan análisis cuantitativos que dan lugar a modelos económicos lineales, es de la programación de la producción de la empresa en el corto plazo con tecnología lineal el que permite formular algunos modelos lineales de compatibilidad y de programación lineal que pueden servir de guía para la formulación de cualquier otro modelo económico lineal. De esta forma, los alumnos adquieren la motivación por la asignatura desde el principio, y conocen algunas de las necesidades matemáticas de la ciencia económica que les permitirá afrontar más satisfactoriamente el resto de la asignatura.

  13. Calendar

    Science.gov (United States)

    2004-04-01

    2004 dkermani@fit.edu, +1 321 674 7412, http://kermani.math.fit.edu/ JULY 2004 2nd World Congress of the Game Theory Society, Faculty of Luminy, Marseille, France 5-9 July 2004 Europa Organisation (europa@europa-organisation.com), +33 5 34 45 26 45, www.gts2004.org Budapest Workshop on Behavioral Economics, Central European University, Budapest, Hungary 5-10 July 2004 Eva Dotzi (behavecon@ceu.hu), www.iza.org/en/calls_conferences/CallCEU_04.pdf FDA'04 1st IFAC Workshop on Fractional Differentiation and its Applications, Bordeaux, France 19-20 July 2004 IFAC secretariat (fda04@lap.u-bordeaux1.fr), www.lap.u-bordeaux.fr/fda04/ Bachelier Finance Society Third World Congress, InterContinental Hotel, Chicago, IL, USA 21-24 July 2004 bfs2004@uic.edu, www.uic.edu/orgs/bachelier/ BS/IMS 2004 6th World Congress of the Bernoulli Society for Mathematical Statistics and Probability, Barcelona, Spain 26-31 July 2004 wc2004@pacifico-meetings.com, +34 93 402 13 85, www.imub.ub.es/events/wc2004 AUGUST 2004 Summer School in Econometrics. The Cointegrated VAR Model: Econometric Methodology and Macroeconomic Applications, Institute of Economics, University of Copenhagen, Denmark 2-22 August 2004 Summerschool@econ.ku.dk, www.econ.ku.dk/summerschool SEPTEMBER 2004 First Bonzenfreies Colloquium on Market Dynamics and Quantitative Economics, Alessandria, Palazzo Borsalino, Italy 9-10 September 2004 colloquium@unipmn.it, www.mfn.unipmn.it/~colloqui/ Risk Analysis 2004. 4th International Conference on Computer Simulation in Risk Analysis and Hazard Mitigation, Aldemar Paradise Royal Mare Hotel, Rhodes, Greece 27-29 September 2004 enquiries@wessex.ac.uk, +44 (0)238 029 3223, www.wessex.ac.uk/conferences/2004/risk04/ OCTOBER 2004 IRC Hedge 2004, InterContinental Hotel, London, UK 10, 11 October 2004 enquiries@irc-conferences.com, www.irc-conferences.com/show_conference.php?id=10 NOVEMBER 2004 IRC DICE 2004, InterContinental Hotel, London, UK 22, 23 November 2004 enquiries

  14. Emergence of a multiresistant KPC-3 and VIM-1 carbapenemase-producing Escherichia coli strain in Spain.

    Science.gov (United States)

    Porres-Osante, Nerea; Azcona-Gutiérrez, Jose Manuel; Rojo-Bezares, Beatriz; Undabeitia, Esther; Torres, Carmen; Sáenz, Yolanda

    2014-07-01

    To characterize the mechanisms involved in carbapenem resistance, as well as the genetic elements supporting their mobilization, in a multidrug-resistant Escherichia coli isolate. The E. coli isolate was obtained from a patient with fatal urinary sepsis. Antimicrobial susceptibility testing was performed by the disc diffusion and agar dilution methods. The E. coli molecular type and phylogroup were determined using multilocus sequence typing and the triple PCR technique, respectively. PCR and sequencing were used for virulence and resistance genotype characterization. Plasmid content and gene location were analysed by S1-PFGE, I-Ceu1-PFGE and hybridization experiments. Transformation assays were performed. The E. coli strain, typed as ST448 and phylogroup B1, was resistant to all tested antibiotics except fosfomycin, tigecycline and tetracycline. The following resistance and virulence genetic structures were obtained: ISKpn7 + bla(KPC-3) + ISKpn6 linked to Tn4401; tnpR + aac(6')-Ib'-9 + aadA1 + bla(OXA-9) + tnpR + bla(TEM-1a) + tnpB + strB + strA + sul2; intI1 + bla(VIM-1) + aac(6')-Ib' + aphA15 + aadA1 + catB2 + qacEΔ1-sul1 + orf5; ISEcp1 + bla(CMY-2); IS26 + bla(SHV-12); aph(3')-I; aac(3)-IV; floR; catA; and fimA. Mutations in the ampC promoter (-18, -1 and +58) and substitutions in the GyrA (Ser-83→Leu and Asp-87→Asn) and ParC (Ser-80→Ile) proteins were observed. IncFII (ST2), IncA/C and ColE(TP) plasmids of 145.5, 87 and 300 kb, and the bla(KPC-3) gene in the 145.5 kb IncFII plasmid. Transformant strains carried the IncFII and ColE(TP) plasmids, and the bla(KPC-3), bla(TEM-1a), bla(OXA-9), aadA1, aac(6')-Ib'-9, aac(3)-IV and floR genes. This is the first report of the co-production of KPC-3, VIM-1, SHV-12, OXA-9 and CMY-2 in a unique clinical multiresistant E. coli isolate. The dissemination of these genes on mobile genetic elements is alarming and complicates antimicrobial

  15. Report on the International Workshop “Networks, Regions and Institutions in Mongol Eurasia: A Meso-Historical Analysis”, Jerusalem, 17–18 May, 2017

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    Roman Hautala

    2017-09-01

    Everyday Life in the Golden Horde (First Half of the 14th Century”; Stephen Pow, (CEU University “What a Source Says, When It Says Nothing at All: Extracting Information from Jochi Khan’s Biography in the 117th Chapter of the Yuan Shi”; Jonathan Brack (The Hebrew University of Jerusalem, “How the Dead Mattered in Ilkhanid Iran? The Local and the Global in Rashid al-Din’s Three Refutations of Reincarnation”. In the framework of the final fourth panel “Law as a Meso-Institution” the following talks were presented: Khohchahar E. Chuluu (The University of Tokyo, “Law, Institutions, and Justice in the Mongol Empire”; Florence Hodous (Renmin University, “Joint Trials as a Key to Local History and Empire-Wide Dynamics”; Edith Chen (Princeton University, “Justice of the Khan: Writing the Lives of the Jarqučis in the Yuan Shi”.

  16. Accuracy of genome-wide imputation of untyped markers and impacts on statistical power for association studies

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    McElwee Joshua

    2009-06-01

    Full Text Available Abstract Background Although high-throughput genotyping arrays have made whole-genome association studies (WGAS feasible, only a small proportion of SNPs in the human genome are actually surveyed in such studies. In addition, various SNP arrays assay different sets of SNPs, which leads to challenges in comparing results and merging data for meta-analyses. Genome-wide imputation of untyped markers allows us to address these issues in a direct fashion. Methods 384 Caucasian American liver donors were genotyped using Illumina 650Y (Ilmn650Y arrays, from which we also derived genotypes from the Ilmn317K array. On these data, we compared two imputation methods: MACH and BEAGLE. We imputed 2.5 million HapMap Release22 SNPs, and conducted GWAS on ~40,000 liver mRNA expression traits (eQTL analysis. In addition, 200 Caucasian American and 200 African American subjects were genotyped using the Affymetrix 500 K array plus a custom 164 K fill-in chip. We then imputed the HapMap SNPs and quantified the accuracy by randomly masking observed SNPs. Results MACH and BEAGLE perform similarly with respect to imputation accuracy. The Ilmn650Y results in excellent imputation performance, and it outperforms Affx500K or Ilmn317K sets. For Caucasian Americans, 90% of the HapMap SNPs were imputed at 98% accuracy. As expected, imputation of poorly tagged SNPs (untyped SNPs in weak LD with typed markers was not as successful. It was more challenging to impute genotypes in the African American population, given (1 shorter LD blocks and (2 admixture with Caucasian populations in this population. To address issue (2, we pooled HapMap CEU and YRI data as an imputation reference set, which greatly improved overall performance. The approximate 40,000 phenotypes scored in these populations provide a path to determine empirically how the power to detect associations is affected by the imputation procedures. That is, at a fixed false discovery rate, the number of cis

  17. Relationships between craniocervical posture and pain-related disability in patients with cervico-craniofacial pain

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    López-de-Uralde-Villanueva I

    2015-07-01

    Full Text Available Ibai López-de-Uralde-Villanueva,1–4 Hector Beltran-Alacreu,1–3 Alba Paris-Alemany,1–4 Santiago Angulo-Díaz-Parreño,2,3,5 Roy La Touche1–4 1Department of Physiotherapy, Faculty of Health Science, 2Research Group on Movement and Behavioral Science and Study of Pain, The Center for Advanced Studies University La Salle, Universidad Autónoma de Madrid, Aravaca, Madrid, Spain; 3Institute of Neuroscience and Craniofacial Pain (INDCRAN, Madrid, Spain; 4Hospital La Paz Institute for Health Research, IdiPAZ, Madrid, Spain; 5Faculty of Medicine, Universidad San Pablo CEU, Madrid, Spain Objectives: This cross-sectional correlation study explored the relationships between craniocervical posture and pain-related disability in patients with chronic cervico-craniofacial pain (CCFP. Moreover, we investigated the test–retest intrarater reliability of two craniocervical posture measurements: head posture (HP and the sternomental distance (SMD. Methods: Fifty-three asymptomatic subjects and 60 CCFP patients were recruited. One rater measured HP and the SMD using a cervical range of motion device and a digital caliper, respectively. The Spanish versions of the neck disability index and the craniofacial pain and disability inventory were used to assess pain-related disability (neck disability and craniofacial disability, respectively. Results: We found no statistically significant correlations between craniocervical posture and pain-related disability variables (HP and neck disability [r=0.105; P>0.05]; HP and craniofacial disability [r=0.132; P>0.05]; SMD and neck disability [r=0.126; P>0.05]; SMD and craniofacial disability [r=0.195; P>0.05]. A moderate positive correlation was observed between HP and SMD for both groups (asymptomatic subjects, r=0.447; CCFP patients, r=0.52. Neck disability was strongly positively correlated with craniofacial disability (r=0.79; P>0.001. The test–retest intrarater reliability of the HP measurement was high for

  18. Design and Testing of a Controller for the Martian Atmosphere Pressure and Humidity Instrument DREAMS-P/H

    Science.gov (United States)

    Tapani Nikkanen, Timo; Schmidt, Walter; Genzer, Maria; Harri, Ari-Matti; Haukka, Harri

    2013-04-01

    The European Space Agency (ESA), driven by the goal of performing a soft landing on Mars, is planning to launch the Entry, descent and landing Demonstrator Module (EDM)[1] simultaneously with the Trace Gas Orbiter (TGO) as a part of the ExoMars program towards Mars in 2016. As a secondary objective, the EDM will gather meteorological data and observe the electrical environment of the landing site with its Dust characterisation, Risk assessment, and Environmental Analyser on the Martian Surface (DREAMS). The Finnish Meteorological Institute (FMI) is participating in the project by designing, building and testing a pressure and a humidity instrument for Mars, named DREAMS-P and DREAMS-H, respectively. The instruments are based on previous FMI designs, including ones flown on board the Huygens, Phoenix and Mars Science Laboratory.[2] Traditionally, the FMI pressure and humidity instruments have been controlled by an FPGA. However, the need to incorporate more autonomy and modifiability into instruments, cut the development time and component costs, stimulated interest to study a Commercial Off-The-Shelf (COTS) Microcontroller Unit (MCU) based instrument design. Thus, in the DREAMS-P/H design, an automotive MCU is used as the instrument controller. The MCU has been qualified for space by tests in and outside FMI. The DREAMS-P/H controller command and data interface utilizes a RS-422 connection to receive telecommands from and to transmit data to the Central Electronics Unit (CEU) of the DREAMS science package. The two pressure transducers of DREAMS-P and one humidity transducer of DREAMS-H are controlled by a single MCU. The MCU controls the power flow for each transducer and performs pulse counting measurements on sensor and reference channels to retrieve scientific data. Pressure and humidity measurements are scheduled and set up according to a configuration table assigned to each transducer. The configuration tables can be modified during the flight. The whole

  19. A review of eating disorders research in Mexico.

    Science.gov (United States)

    Unikel, Claudia; Bojorquez, Ietza

    2007-02-01

    indizadas y tesis de grado, mediante los descriptores "trastorno alimentario," anorexia nervosa," bulimia nervosa," "imagen corporal," "atracones," "restricción alimentaria," "preocupación por el peso y la figura," y "conducta de dieta." Éstos se combinaron con el operador Booleano "AND" con "México" y "América Latina." Se presentan los hallazgos para la epidemiología, la validez de los instrumentos de medición, comorbilidad, y factores de riesgo. Una encuesta nacional representativa encontró una prevalencia de 1.8% para la bulimia nervosa, y ningún caso de anorexia nervosa. No obstante, la carencia de estudios con una entrevista clínica confirmatoria y de otras muestras nacionales o regionales dificulta el arribo a conclusiones acerca de la prevalencia real. Varios instrumentos para la detección de los trastornos alimentarios y de la alimentación desordenada se han validado en población mexicana. La comorbilidad de los trastornos alimentarios en México incluye el uso y abuso de alcohol, la obesidad y el trastorno de personalidad limítrofe. Los factores de riesgo referidos incluyen el peso corporal y la presión cultural para estar delgado. Las líneas de investigación futuras deberán incluir estudios epidemiológicos con muestras representativas y la confirmación del diagnóstico, estudios longitudinales, y la exploración de factores de riesgo y protectores específicos a esta población. L'objectif de cet article est de résumer les résultats de recherche et l'état actuel des connaissances sur les troubles alimentaires au Mexique. Les articles publiés dans des revues indexées et les thèses de doctorat ont été recensés en utilisant les mots-clés « eating disorders », « anorexia nervosa », « bulimia nervosa », « body image », « binge eating », « restrained eating », « weight and shape concern » et « dieting ». Ceu

  20. Effective strategy making: Co-designing scenarios as a tool for effective strategic planning

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    Jan Vogelij

    2015-08-01

    approaches the process positively and supportive, although without unconditional commitment to any outcome of the process. The empiric part contains case studies of five strategy making processes in different European countries.The selection of cases was based on a pre-selection proposed by planners, representing their countries in the European Council of Spatial Planners (ECTP-CEU They responded to the request for good examples of cases of successful strategy making in their countries. The final selection of five was chosen applying practical considerations about expectations of local help in assembling information. The selected cases were: Piano Strutturale Comunale of Bologna (IT, Scenariostudie Drechtsteden 2030 (NL, the Glasgow and the Clyde Valley Strategic Development Plan (UK, Vision Gherdëina (IT and Meetjesland 2020 (BE. Next to plan documents and existing evaluations, forty-one interviews formed important sources of information for connecting casuistic and theory. Applying our definition of effectiveness, first the effectiveness of our cases was established. Not all of those five appeared to be effective according to our criteria as formulated in the theoretical frame. Next the cases were analysed, using pre-formulated hypotheses for process and place-related conditions influencing the performance of spatial development strategies. The accounts of cases in different planning cultures related to the process and place-aspects of the theoretic frame provided insight in the different process aspects and the circumstances in which those processes were conducted. That, in combination with the earlier established (non- effectiveness enabled characterizing the processes within their various administrative and societal environments. Subsequently the process aspects and the place-related aspects of the five cases were compared and analysed applying the research questions, the hypotheses and the chains of evidence. Such comparison allowed for conclusions about factors for

  1. Effective strategy making: Co-designing scenarios as a tool for effective strategic planning

    Directory of Open Access Journals (Sweden)

    Jan Vogelij

    2015-08-01

    level approaches the process positively and supportive, although without unconditional commitment to any outcome of the process. The empiric part contains case studies of five strategy making processes in different European countries. The selection of cases was based on a pre-selection proposed by planners, representing their countries in the European Council of Spatial Planners (ECTP-CEU They responded to the request for good examples of cases of successful strategy making in their countries. The final selection of five was chosen applying practical considerations about expectations of local help in assembling information. The selected cases were: Piano Strutturale Comunale of Bologna (IT, Scenariostudie Drechtsteden 2030 (NL, the Glasgow and the Clyde Valley Strategic Development Plan (UK, Vision Gherdëina (IT and Meetjesland 2020 (BE. Next to plan documents and existing evaluations, forty-one interviews formed important sources of information for connecting casuistic and theory. Applying our definition of effectiveness, first the effectiveness of our cases was established. Not all of those five appeared to be effective according to our criteria as formulated in the theoretical frame. Next the cases were analysed, using pre-formulated hypotheses for process and place-related conditions influencing the performance of spatial development strategies. The accounts of cases in different planning cultures related to the process and place-aspects of the theoretic frame provided insight in the different process aspects and the circumstances in which those processes were conducted. That, in combination with the earlier established (non- effectiveness enabled characterizing the processes within their various administrative and societal environments. Subsequently the process aspects and the place-related aspects of the five cases were compared and analysed applying the research questions, the hypotheses and the chains of evidence. Such comparison allowed for conclusions about