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Sample records for ceu infekciju izraistju

  1. From a CEU '98er: 9 years and 5 research projects later

    Science.gov (United States)

    Aidala, Christine

    2007-10-01

    Since my first research experience in 1996 working in low-energy nuclear structure, the results of which were presented at the original DNP CEU poster session in Santa Fe in 1998, subsequent projects led me to weave my way through various energies and collision systems in nuclear and particle physics. Through the course of the broad exposure to research that I have been fortunate enough to experience, I have found a niche for myself in the study of nucleon spin structure. I originally got involved in the field in 1998-99 through my undergraduate senior project on studies for polarizing the proton beam at HERA in Hamburg, Germany. After a foray into particle physics followed by an unanticipated diversion from research, teaching music and English abroad, fate--and some kind individuals--would give me the opportunity to return both to physics and specifically to nucleon structure in 2001 as part of the PHENIX experiment at the Relativistic Heavy Ion Collider. Six years into my research on proton spin structure as a member of the PHENIX Collaboration, I will discuss where I am today and the non-linear path that brought me here.

  2. Competencias profesionales y empleo en el futuro periodista: el caso de los estudiantes de Periodismo de la Universidad Cardenal Herrera-CEU

    Directory of Open Access Journals (Sweden)

    Javier Sierra Sánchez

    2010-12-01

    Full Text Available Presentamos un trabajo de investigación científica enfocado en dos aspectos: la adquisición de competencias profesionales en los alumnos de la licenciatura de Periodismo de la Universidad Cardenal Herrera-CEU y la importancia que otorgan a diferentes aspectos de un empleo. Éste último aspecto, partiendo del Informe Reflex de ANECA “El profesional flexible en la Sociedad del Conocimiento” establecemos una comparativa entre los resultados allí obtenidos y el caso particular de los alumnos de Periodismo de la Cardenal Herrera-CEU. Para descubrir estos aspectos presentamos un trabajo empírico para conocer en qué medida los alumnos de último curso de la licenciatura de la Universidad Cardenal Herrera-CEU han adquirido las competencias profesionales que se reflejan en el Libro Blanco de ANECA de los títulos de grado en Comunicación . Este estudio que presentamos nace con vocación de continuidad en un doble sentido: ser un indicador de la calidad de las enseñanzas que reciben los alumnos de Periodismo de la Universidad Cardenal Herrera-CEU y conocer en un futuro próximo (cuando salga la primera promoción de graduados si existe una diferencia significativa entre los alumnos que cursaron la licenciatura y los nuevos graduados fruto de la reforma universitaria. De este modo, podremos conocer hasta qué punto y qué grado se ha acometido en la Universidad Cardenal Herrera-CEU esa transformación que conlleva el Plan Bolonia en su sentido más profundo de la reforma.

  3. Competencias profesionales y empleo en el futuro periodista: el caso de los estudiantes de periodismo de la Universidad San Pablo CEU

    Directory of Open Access Journals (Sweden)

    Javier Sierra Sánchez

    2012-04-01

    Full Text Available La Universidad española ha sufrido una gran transformación desde su adaptación al EEES. El Plan Bolonia propone un replanteamiento de la misión que debe cumplir la Universidad acercando dicha institución a los intereses y necesidades de la empresa y de la sociedad. Uno de los pilares básicos de estos cambios es el que hace referencia a la formación del alumno por competencias. Presentamos un trabajo de investigación científica enfocado en dos aspectos: la adquisición de competencias profesionales en los alumnos de la licenciatura de Periodismo de la Universidad San Pablo CEU y  la importancia que otorgan a diferentes aspectos de un empleo. Éste último aspecto, partiendo del Informe Réflex de ANECA “El profesional flexible en la Sociedad del Conocimiento” establecemos una comparativa entre los resultados allí obtenidos y el caso particular de los alumnos de Periodismo de la San Pablo CEU. Para descubrir estos aspectos presentamos un trabajo empírico para conocer en qué medida los alumnos de último curso de la licenciatura (que no de grado de la Universidad San Pablo CEU han adquirido las competencias profesionales que se reflejan en el Libro Blanco de ANECA de los títulos de grado en Comunicación.Este estudio que presentamos nace con vocación de continuidad en un doble sentido: ser un indicador de la calidad de las enseñanzas que reciben los alumnos de Periodismo de la San Pablo CEU y conocer en un futuro próximo (cuando salga la primera promoción de graduados si existe una diferencia significativa entre los alumnos que cursaron la licenciatura y los nuevos graduados fruto de la reforma universitaria. De este modo, podremos conocer hasta qué punto y qué grado se ha acometido en la Universidad San Pablo CEU esa transformación que conlleva el Plan Bolonia en su sentido más profundo de la reforma.

  4. El Proceso de Renovación Docente: qué es, qué persigue y su aplicación en la Universidad CEU San Pablo

    Directory of Open Access Journals (Sweden)

    Escribano Ródenas, Mª del Carmen

    2007-01-01

    Full Text Available La adaptación al Espacio Europeo de Educación Superior (EEES implica cambios sustanciales en la concepción de la metodología docente. Así pues, nos encontramos ante un verdadero Proceso de Renovación Docente.Debe destacarse que no se trata de un mero cambio organizativo sino que, el EEES supone una nueva definición de objetivos en términos de competencias y además, se pretende que el centro del proceso formativo sea el aprendizaje activo del alumno.Es indudable que una Universidad privada como la CEU San Pablo, siempre preocupada en lograr los mayores niveles posibles de calidad y excelencia académica, no puede quedar al margen de este proceso de renovación que, sin lugar a dudas, constituye un verdadero desafío que tenemos que afrontar no sólo los profesores sino también los alumnos, ambos pilares fundamentales para mantener activo el cambio que se avecina y lograr los mejores resultados de su correcta aplicación y puesta en funcionamiento.El presente trabajo pretende explicar cuál es la esencia del cambio docente, qué significa fijar objetivos en términos de competencias, qué implican los nuevos objetivos y los nuevos métodos docentes, cuál es el sistema apropiado de evaluación del aprendizaje, cómo abordar el cambio en las asignaturas, y en nuestro caso concreto en la asignatura Matemáticas, y cuál es el protagonismo que poseen los estudiantes.

  5. Metanephric Adenofibroma associated with Papillary Renal CeU Carcinoma

    International Nuclear Information System (INIS)

    Roa, Carmen Lucia B; Navarrete, Maria Constanza

    2008-01-01

    Metanephric adenofibroma is an infrequent biphasic epithelial-stromal renal tumor, occasionally associated with papillary renal cell carcinoma. We describe a case of a girl with a four year clinical history of intermittent hematuria; she was diagnosed, using a left-side tru-cut renal biopsy, with a Wilms' tumor with stromal and epithelial component, with no sign of anaplasia. Later, through the product of the left-side nephrectomy that was performed at the National Cancer institute of Colombia, she was diagnosed with metanephric adenofibroma associated with papillary renal cell carcinoma

  6. CEU.gif"/> The construction of work–life balance: The experience of Black employees in a call-centre environment

    OpenAIRE

    Sonia C.B. Potgieter; Antoni Barnard

    2010-01-01

    Orientation: Work–life balance, as a crucial aspect of employee and organisational wellness, remains an interesting field of research, especially due to the changing demographic employee profile.Research purpose: The objective of the study was to explore Black employees’ construction of work–life balance in a customer care environment.Motivation for the study: The conceptual debate regarding the construct of work–life balance in general as well as limited qualitative research with regard to B...

  7. Lighting measurement station: measurements of natural lighting for characterization of sky and zenith illuminance; Estacao de medicao de iluminacao: medicao de niveis de iluminacao natural para caracterizacao de iluminancias de ceu e luminancia de zenite

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Roberta Vieira Goncalves de [Minas Gerais Univ., Belo Horizonte, MG (Brazil). Escola de Arquitetura. Dept. de Tecnologia da Arquitetura e do Urbanismo]. E-mail: roberta@arq.ufmg.br; Pereira, Fernando Oscar Ruttkay [Santa Catarina Univ., Florianopolis, SC (Brazil). Dept. de Arquitetura. Lab. de Conforto Ambiental]. E-mail: feco@arq.ufsc.br

    2002-07-01

    This paper describes the assembly of a standard station for measuring the day lighting for evaluation of the natural light availability. The mounting and data obtention form will also be described from a standard IDMP day lighting station for measuring the data from measuring zenith, horizontal, and vertical illuminance of the sky and vertical irradiance for the cardinal points.

  8. Formación de especialistas sanitarios (FIR de Farmacia Industrial y Galénica de la Unidad Docente de la Universidad CEU San Pablo tras estancias en la industria farmacéutica de Madrid

    Directory of Open Access Journals (Sweden)

    Luis Alberto del Río

    2015-10-01

    La incorporación profesional de los farmacéuticos industriales demuestra una alta tasa de empleo dentro de la industria madrileña, incluso antes de terminar su período de formación en la mayoría de los casos. La formación recibida en la Escuela ha sido útil para conseguir empleo de forma rápida y con una responsabilidad relacionada con la formación recibida. Asimismo, se ha producido un aumento en la duración de las estancias en los laboratorios madrileños, lo que ha permitido a los titulados una mayor experiencia y vínculo profesional.

  9. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture Series All Training Information News & Events Press ...

  10. Tai Chi and Qi Gong for Health and Well-Being

    Medline Plus

    Full Text Available ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture Series All Training Information News & Events Press ...

  11. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... Resources CME/CEU and Online Lectures Online Continuing Education ... newsletter with evidence-based information on complementary and integrative practices and a health condition ...

  12. Tai Chi and Qi Gong for Health and Well-Being

    Medline Plus

    Full Text Available ... Resources CME/CEU and Online Lectures Online Continuing Education ... newsletter with evidence-based information on complementary and integrative practices and a health condition ...

  13. Clinical Epidemiology Unit - overview of research areas

    Science.gov (United States)

    Clinical Epidemiology Unit (CEU) conducts etiologic research with potential clinical and public health applications, and leads studies evaluating population-based early detection and cancer prevention strategies

  14. Addendum: Pardo, J.; Zamora-Martínez, F.; Botella-Rocamora, P. Online Learning Algorithm for Time Series Forecasting Suitable for Low Cost Wireless Sensor Networks Nodes. Sensors 2015, 15, 9277–9304

    Directory of Open Access Journals (Sweden)

    Juan Pardo

    2015-07-01

    Full Text Available This work has been supported by the Consolidación de indicadores CEU-UCH2014-15 program of the Vicerrectorado de Investigación at Universidad CEU-Cardenal Herrera and financed by Generalitat Valenciana through Conselleria de Educación, Cultura y Deporte under project GV/2015/088. [...

  15. "Combining equity and utilitarianism"-additional insights into a novel approach

    NARCIS (Netherlands)

    Lemmen-Gerdessen, van Joke; Kanellopoulos, Argyris; Claassen, Frits

    2018-01-01

    Recently, a novel approach (to be referred to as CEU) was introduced for the frequently arising problem of combining the conflicting criteria of equity and utilitarianism. This paper provides additional insights into CEU and assesses its added value for practice by comparing it with a commonly used

  16. RINITIS U ODRASLIH

    OpenAIRE

    KALOGJERA, LIVIJE

    2011-01-01

    Rinitis je upala nosne sluznice različite etiologije, uključujući infekciju, alergiju ili hiperreaktivni odgovor na raznovrsne podražaje iz okoline. Praćen je simptomima opstrukcije, hipersekrecije, kihanja ili svrbeža nosa. Klasifikacije rinitisa se oslanjaju na etiologiju i težinu simptoma. Iako je s epidemiološkog gledišta najčešći virusni rinitis u sklopu obične prehlade, javnozdravstveno se značenje prvenstveno fokusira na kronični rinitis, alergijski i nealergijski. Alergijski rinitis s...

  17. Prognostički značaj određivanja koncentracija citokina članova superfamilije tumor nekrozis faktora alfa kod obolelih od sepse

    OpenAIRE

    Lendak, Dajana

    2015-01-01

    Uvod: Nespecifičnost kliničke slike sepse, velike individualne razlike u odgovoru organizma na infekciju kao i neophodnost adekvatne inicijalne procene težine kliničke slike, toka i ishoda bolesti, čine istraživanja biomarkera koji bi doprineli pravovremenom postavljanju dijagnoze i adekvatnoj prognozi bolesti izuzetno značajnim. Do sada je ispitivano preko 200 biomarkera od kojih ni jedan nije pokazao zadovoljavajuću senzitivnost i specifičnost. Uloga B limfocita u patogenezi sepse pri tome ...

  18. Tai Chi and Qi Gong for Health and Well-Being

    Medline Plus

    Full Text Available ... All Grant Information Training About Research Training and Career Development Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU ...

  19. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... All Grant Information Training About Research Training and Career Development Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU ...

  20. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine ... 37sec The video from NCCIH includes: A look at innovative technology that examines how older people use their muscles ...

  1. Tai Chi and Qi Gong for Health and Well-Being

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    Full Text Available ... For Health Care Professionals Clinical Practice Guidelines Literature Reviews All Health Information Research Research Results Results by ... Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU ...

  2. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... For Health Care Professionals Clinical Practice Guidelines Literature Reviews All Health Information Research Research Results Results by ... Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU ...

  3. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture Series All Training Information News & Events Press Releases Alerts & Advisories Events Multimedia ( ...

  4. Tai Chi and Qi Gong for Health and Well-Being

    Medline Plus

    Full Text Available ... Mind and Body Research Pain Research All Grant Information Training About Research Training and Career Development Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU and Online Lectures ...

  5. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... Mind and Body Research Pain Research All Grant Information Training About Research Training and Career Development Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU and Online Lectures ...

  6. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture Series All Training Information News & Events Press ...

  7. Tai Chi and Qi Gong for Health and Well-Being

    Medline Plus

    Full Text Available ... Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture Series All Training Information News & Events Press ...

  8. Sleep Disorders and Complementary Health Approaches

    Science.gov (United States)

    ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ... R, Sarris J, Schweitzer I. Kava hepatotoxicity in traditional and modern use: the presumed Pacific kava paradox hypothesis revisited. ...

  9. NCCAM's 5 Most Searched-For Herbs of 2012: What the Science Says about Evening Primrose Oil, St. John's Wort, Fenugreek,...

    Science.gov (United States)

    ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ... has been a source of many folk or traditional remedies and more modern medicinal and cosmetic products. At various times aloe ...

  10. Traditional Chinese Medicine: An Introduction

    Science.gov (United States)

    ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ... TCM, it is important to separate questions about traditional theories and ... of modern science-based medicine and health promotion practices. The ...

  11. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... Science For Health Care Professionals Clinical Practice Guidelines Literature Reviews All Health Information Research Research Results Results ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ...

  12. Tai Chi and Qi Gong for Health and Well-Being

    Medline Plus

    Full Text Available ... Science For Health Care Professionals Clinical Practice Guidelines Literature Reviews All Health Information Research Research Results Results ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ...

  13. Tai Chi and Qi Gong for Health and Well-Being

    Medline Plus

    Full Text Available ... of Grants and Contracts General Award Mechanisms Small Business Research Grant Program (SBIR) ... CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series ...

  14. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... of Grants and Contracts General Award Mechanisms Small Business Research Grant Program (SBIR) ... CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series ...

  15. [A pragmatic alliance. Jewisch-Lithuanian political cooperation at the beginning of the 20th century. Hrsg. von Vladas Sirutavičius, Darius Staliūnas] / Karsten Brüggemann

    Index Scriptorium Estoniae

    Brüggemann, Karsten, 1965-

    2013-01-01

    Arvustus: A pragmatic alliance. Jewisch-Lithuanian political cooperation at the beginning of the 20th century. Hrsg. von Vladas Sirutavičius und Darius Staliūnas. Verlag CEU Press. Budapest und New York, 2011

  16. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series ...

  17. Tai Chi and Qi Gong for Health and Well-Being

    Medline Plus

    Full Text Available ... Awards & Opportunities Institutional Training Sites Training Grant Application, Review, and Award Process More Training Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series ...

  18. Tai Chi and Qi Gong for Health and Well-Being

    Medline Plus

    Full Text Available ... Safety Information Know the Science For Health Care Professionals Clinical Practice Guidelines Literature Reviews All Health Information ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ...

  19. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... Safety Information Know the Science For Health Care Professionals Clinical Practice Guidelines Literature Reviews All Health Information ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ...

  20. Antibiotiku aprite Benu aptiekā 7

    OpenAIRE

    Mičule, Baiba

    2013-01-01

    Antibiotikas plaši pielieto infekciju slimību ārstēšanā. Bieži vien ārsti antibiotikas izraksta arī vīrusa infekcijas gadījumā, tādēļ pieaug antibiotiku patēriņš un aizvien vairāk tiek runāts par antibiotiku rezistences veidošanos, kā rezultātā baktērijas kļūst nejūtīgas pret antibakteriālajiem līdzekļiem. Bakalaura darba mērķis bija izpētīt perorāli lietojamo antibiotiku apriti BENU aptiekā 7, laika posmā no 2011. gada 1. janvāra līdz 2012. gada 31. decembrim. Veicot pētījumu, tika no...

  1. Simulating the characteristics of tropical cyclones over the South West Indian Ocean using a Stretched-Grid Global Climate Model

    Science.gov (United States)

    Maoyi, Molulaqhooa L.; Abiodun, Babatunde J.; Prusa, Joseph M.; Veitch, Jennifer J.

    2018-03-01

    Tropical cyclones (TCs) are one of the most devastating natural phenomena. This study examines the capability of a global climate model with grid stretching (CAM-EULAG, hereafter CEU) in simulating the characteristics of TCs over the South West Indian Ocean (SWIO). In the study, CEU is applied with a variable increment global grid that has a fine horizontal grid resolution (0.5° × 0.5°) over the SWIO and coarser resolution (1° × 1°—2° × 2.25°) over the rest of the globe. The simulation is performed for the 11 years (1999-2010) and validated against the Joint Typhoon Warning Center (JTWC) best track data, global precipitation climatology project (GPCP) satellite data, and ERA-Interim (ERAINT) reanalysis. CEU gives a realistic simulation of the SWIO climate and shows some skill in simulating the spatial distribution of TC genesis locations and tracks over the basin. However, there are some discrepancies between the observed and simulated climatic features over the Mozambique channel (MC). Over MC, CEU simulates a substantial cyclonic feature that produces a higher number of TC than observed. The dynamical structure and intensities of the CEU TCs compare well with observation, though the model struggles to produce TCs with a deep pressure centre as low as the observed. The reanalysis has the same problem. The model captures the monthly variation of TC occurrence well but struggles to reproduce the interannual variation. The results of this study have application in improving and adopting CEU for seasonal forecasting over the SWIO.

  2. Real-time contrast ultrasound muscle perfusion imaging with intermediate-power imaging coupled with acoustically durable microbubbles.

    Science.gov (United States)

    Seol, Sang-Hoon; Davidson, Brian P; Belcik, J Todd; Mott, Brian H; Goodman, Reid M; Ammi, Azzdine; Lindner, Jonathan R

    2015-06-01

    There is growing interest in limb contrast-enhanced ultrasound (CEU) perfusion imaging for the evaluation of peripheral artery disease. Because of low resting microvascular blood flow in skeletal muscle, signal enhancement during limb CEU is prohibitively low for real-time imaging. The aim of this study was to test the hypothesis that this obstacle can be overcome by intermediate- rather than low-power CEU when performed with an acoustically resilient microbubble agent. Viscoelastic properties of Definity and Sonazoid were assessed by measuring bulk modulus during incremental increases in ambient pressure to 200 mm Hg. Comparison of in vivo microbubble destruction and signal enhancement at a mechanical index (MI) of 0.1 to 0.4 was performed by sequential reduction in pulsing interval from 10 to 0.05 sec during limb CEU at 7 MHz in mice and 1.8 MHz in dogs. Destruction was also assessed by broadband signal generation during passive cavitation detection. Real-time CEU perfusion imaging with destruction-replenishment was then performed at 1.8 MHz in dogs using an MI of 0.1, 0.2, or 0.3. Sonazoid had a higher bulk modulus than Definity (66 ± 12 vs 29 ± 2 kPa, P = .02) and exhibited less inertial cavitation (destruction) at MIs ≥ 0.2. On in vivo CEU, maximal signal intensity increased incrementally with MI for both agents and was equivalent between agents except at an MI of 0.1 (60% and 85% lower for Sonazoid at 7 and 1.8 MHz, respectively, P power imaging coupled with a durable microbubble contrast agent. Copyright © 2015 American Society of Echocardiography. All rights reserved.

  3. Die „lex CEU“: der Tropfen, der das Fass zum Überlaufen bringt? Die ungarische Normsetzung an der Grenze des Rechtsstaates / Zoltán Rónay

    Index Scriptorium Estoniae

    Rónay, Zoltán

    2017-01-01

    Ungari kõrgharidusseaduse ja teiste seaduse muudatustest, mis puudutavad ka Kesk-Euroopa Ülikooli (seaduse hüüdnimi Lex CEU). Selle seaduse vastavusest õigusriigi põhimõtetele. Seaduse tekstid saksa keeles lk. 389-395

  4. Experiment list: ERX329712 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available H || sex=female || 1000 Genomes dataset=Pilot2 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bw/E...apiens || cell type=lymphoblastoid cell || cell line=NA12878 || population=HapMap CEU || population name=CEP

  5. Experiment list: ERX329709 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available H || sex=female || 1000 Genomes dataset=Pilot2 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bw/E...apiens || cell type=lymphoblastoid cell || cell line=NA12878 || population=HapMap CEU || population name=CEP

  6. Experiment list: ERX329660 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available || sex=female || 1000 Genomes dataset=Pilot2 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/eachData/bw/ER...piens || cell type=lymphoblastoid cell || cell line=NA12878 || population=HapMap CEU || population name=CEPH

  7. Discrimination between Native and Tn6010-Associated oqxAB in Klebsiella spp., Raoultella spp., and other Enterobacteriaceae by Using a Two-Step Strategy

    DEFF Research Database (Denmark)

    Guillard, Thomas; Lebreil, Anne-Laure; Hansen, Lars Hestbjerg

    2015-01-01

    ABSTRACT We developed a two-step PCR-based strategy to detect genes encoding OqxAB, allowing a specific assignment of Tn6010-associated oqxAB in Enterobacteriaceae. Chromosomal location in this setup was confirmed by hybridization with I-CeuI-restricted genomes. This approach led us to find...

  8. Scientific Results of Yoga for Health and Well-Being

    Medline Plus

    Full Text Available ... Resources CME/CEU and Online Lectures Online Continuing Education Series Distinguished Lecture Series Integrated Medicine Research Lecture ... Clinical Digest A monthly newsletter with evidence-based information on complementary and integrative practices and a health condition All News & ... 37sec The video from NCCIH includes: A look at innovative technology that examines how older people use their muscles ...

  9. FREEDOM OF EXPRESSION IN ETHIOPIA: THE ...

    African Journals Online (AJOL)

    eliasn

    freedom of expression as provided under the FDRE Constitution. Then, some ... M (CEU); Addis Ababa University School of Law, Currently on .... The other line of reasoning that is adopted to justify the protection of speech is one that makes ...

  10. Rest-Stress Limb Perfusion Imaging in Humans with Contrast Ultrasound Using Intermediate-Power Imaging and Microbubbles Resistant to Inertial Cavitation.

    Science.gov (United States)

    Davidson, Brian P; Hodovan, James; Belcik, J Todd; Moccetti, Federico; Xie, Aris; Ammi, Azzdine Y; Lindner, Jonathan R

    2017-05-01

    Contrast-enhanced ultrasound (CEU) limb perfusion imaging is a promising approach for evaluating peripheral artery disease (PAD). However, low signal enhancement in skeletal muscle has necessitated high-power intermittent imaging algorithms, which are not clinically feasible. We hypothesized that CEU using a combination of intermediate power and a contrast agent resistant to inertial cavitation would allow real-time limb stress perfusion imaging. In normal volunteers, CEU of the calf skeletal muscle was performed on separate days with Sonazoid, Optison, or Definity. Progressive reduction in the ultrasound pulsing interval was used to assess the balance between signal enhancement and agent destruction at escalating mechanical indices (MI, 0.1-0.4). Real-time perfusion imaging at MI 0.1-0.4 using postdestructive replenishment kinetics was performed at rest and during 25 W plantar flexion contractile exercise. For Optison, limb perfusion imaging was unreliable at rest due to very low signal enhancement generated at all MIs and was possible during exercise-induced hyperemia only at MI 0.1 due to agent destruction at higher MIs. For Definity, signal intensity progressively increased with MI but was offset by microbubble destruction, which resulted in modest signal enhancement during CEU perfusion imaging and distortion of replenishment curves at MI ≥ 0.2. For Sonazoid, there strong signal enhancement at MI ≥ 0.2, with little destruction detected only at MI 0.4. Accordingly, high signal intensity and nondistorted perfusion imaging was possible at MI 0.2-0.3 and detected an 8.0- ± 5.7-fold flow reserve. Rest-stress limb perfusion imaging in humans with real-time CEU, which requires only seconds to perform, is possible using microbubbles with viscoelastic properties that produce strong nonlinear signal generation without destruction at intermediate acoustic pressures. Copyright © 2016 American Society of Echocardiography. All rights reserved.

  11. UNICOAT. Development Laboratory Characterization and Field Evaluation

    Science.gov (United States)

    1990-03-30

    PROBLEMS 4bD 4E2UIRED AIhTE4HANCE THE IMPORTANCE OF THIS MATERIAL IS T4aT IT WILL PROVIDE AN ALTERNATIVE SOURCE FOR A FLEVIBLE PRiMER. THIS PRiMED IS...ICCA’- on tiis 9-!4 i𔃿 was e:Lfflient. NADEP Norfolk personnel tD . Cddmani iriiortei NAD (C..-eu oi ttog snort out 1,+e. tor. CcidMAn ,totaed that

  12. Apo and Iron Bound Fur Repression and the Role of Fur in vivo

    Science.gov (United States)

    2010-03-24

    transport (frpB, ceuE) (257). IG-443 is encoded in the intergenic region between fur and HP1033, and is predicted to regulate the flagellar motor ...relationship with the development of gastroesophageal reflux disease (33), Barrett’s esophagus (64), esophageal adenocarcinoma (73), diarrheal diseases (55...active tuberculosis (51), asthma, and other allergic disorders (7, 13, 53). Because of this, a vaccination or therapeutic approach to abrogate H

  13. Extent and distribution of linkage disequilibrium in the Old Order Amish.

    Science.gov (United States)

    Van Hout, Cristopher V; Levin, Albert M; Rampersaud, Evadnie; Shen, Haiqing; O'Connell, Jeffrey R; Mitchell, Braxton D; Shuldiner, Alan R; Douglas, Julie A

    2010-02-01

    Knowledge of the extent and distribution of linkage disequilibrium (LD) is critical to the design and interpretation of gene mapping studies. Because the demographic history of each population varies and is often not accurately known, it is necessary to empirically evaluate LD on a population-specific basis. Here we present the first genome-wide survey of LD in the Old Order Amish (OOA) of Lancaster County Pennsylvania, a closed population derived from a modest number of founders. Specifically, we present a comparison of LD between OOA individuals and US Utah participants in the International HapMap project (abbreviated CEU) using a high-density single nucleotide polymorphism (SNP) map. Overall, the allele (and haplotype) frequency distributions and LD profiles were remarkably similar between these two populations. For example, the median absolute allele frequency difference for autosomal SNPs was 0.05, with an inter-quartile range of 0.02-0.09, and for autosomal SNPs 10-20 kb apart with common alleles (minor allele frequency > or =0.05), the LD measure r(2) was at least 0.8 for 15 and 14% of SNP pairs in the OOA and CEU, respectively. Moreover, tag SNPs selected from the HapMap CEU sample captured a substantial portion of the common variation in the OOA ( approximately 88%) at r(2) > or =0.8. These results suggest that the OOA and CEU may share similar LD profiles for other common but untyped SNPs. Thus, in the context of the common variant-common disease hypothesis, genetic variants discovered in gene mapping studies in the OOA may generalize to other populations. 2009 Wiley-Liss, Inc.

  14. Geographical affinities of the HapMap samples.

    Directory of Open Access Journals (Sweden)

    Miao He

    Full Text Available The HapMap samples were collected for medical-genetic studies, but are also widely used in population-genetic and evolutionary investigations. Yet the ascertainment of the samples differs from most population-genetic studies which collect individuals who live in the same local region as their ancestors. What effects could this non-standard ascertainment have on the interpretation of HapMap results?We compared the HapMap samples with more conventionally-ascertained samples used in population- and forensic-genetic studies, including the HGDP-CEPH panel, making use of published genome-wide autosomal SNP data and Y-STR haplotypes, as well as producing new Y-STR data. We found that the HapMap samples were representative of their broad geographical regions of ancestry according to all tests applied. The YRI and JPT were indistinguishable from independent samples of Yoruba and Japanese in all ways investigated. However, both the CHB and the CEU were distinguishable from all other HGDP-CEPH populations with autosomal markers, and both showed Y-STR similarities to unusually large numbers of populations, perhaps reflecting their admixed origins.The CHB and JPT are readily distinguished from one another with both autosomal and Y-chromosomal markers, and results obtained after combining them into a single sample should be interpreted with caution. The CEU are better described as being of Western European ancestry than of Northern European ancestry as often reported. Both the CHB and CEU show subtle but detectable signs of admixture. Thus the YRI and JPT samples are well-suited to standard population-genetic studies, but the CHB and CEU less so.

  15. Cervical cancer screening in adolescents: an evidence-based internet education program for practice improvement among advanced practice nurses.

    Science.gov (United States)

    Choma, Kim; McKeever, Amy E

    2015-02-01

    The literature reports great variation in the knowledge levels and application of the recent changes of cervical cancer screening guidelines into clinical practice. Evidence-based screening guidelines for the prevention and early detection of cervical cancer offers healthcare providers the opportunity to improve practice patterns among female adolescents by decreasing psychological distress as well as reducing healthcare costs and morbidities associated with over-screening. The purpose of this pilot intervention study was to determine the effects of a Web-based continuing education unit (CEU) program on advanced practice nurses' (APNs) knowledge of current cervical cancer screening evidence-based recommendations and their application in practice. This paper presents a process improvement project as an example of a way to disseminate updated evidence-based practice guidelines among busy healthcare providers. This Web-based CEU program was developed, piloted, and evaluated specifically for APNs. The program addressed their knowledge level of cervical cancer and its relationship with high-risk human papillomavirus. It also addressed the new cervical cancer screening guidelines and the application of those guidelines into clinical practice. Results of the study indicated that knowledge gaps exist among APNs about cervical cancer screening in adolescents. However, when provided with a CEU educational intervention, APNs' knowledge levels increased and their self-reported clinical practice behaviors changed in accordance with the new cervical cancer screening guidelines. Providing convenient and readily accessible up-to-date electronic content that provides CEU enhances the adoption of clinical practice guidelines, thereby decreasing the potential of the morbidities associated with over-screening for cervical cancer in adolescents and young women. © 2014 Sigma Theta Tau International.

  16. Noninvasive ultrasound molecular imaging of the effect of statins on endothelial inflammatory phenotype in early atherosclerosis.

    Directory of Open Access Journals (Sweden)

    Elham Khanicheh

    Full Text Available BACKGROUND/OBJECTIVES: Inflammatory changes on the endothelium are responsible for leukocyte recruitment to plaques in atherosclerosis. Noninvasive assessment of treatment-effects on endothelial inflammation may be of use for managing medical therapy and developing novel therapies. We hypothesized that molecular imaging of vascular cell adhesion molecule-1 (VCAM-1 with contrast enhanced ultrasound (CEU could assess treatment effects on endothelial phenotype in early atherosclerosis. METHODS: Mice with atherosclerosis produced by gene deletion of the LDL-receptor and Apobec-1-editing protein were studied. At 12 weeks of age, mice received 8 weeks of regular chow or atorvastatin-enriched chow (10 mg/kg/day. At 20 weeks, CEU molecular imaging for aortic endothelial VCAM-1 expression was performed with VCAM-1-targeted (MB(VCAM and control microbubbles (MB(Ctr. Aortic wall thickness was assessed with high frequency ultrasound. Histology, immunohistology and Western blot were used to assess plaque burden and VCAM-1 expression. RESULTS: Plaque burden was reduced on histology, and VCAM-1 was reduced on Western blot by atorvastatin, which corresponded to less endothelial expression of VCAM-1 on immunohistology. High frequency ultrasound did not detect differences in aortic wall thickness between groups. In contrast, CEU molecular imaging demonstrated selective signal enhancement for MB(VCAM in non-treated animals (MB(VCAM 2±0.3 vs MB(Ctr 0.7±0.2, p<0.01, but not in statin-treated animals (MB(VCAM 0.8±0.2 vs MB(Ctr 1.0±0.2, p = ns; p<0.01 for the effect of statin on MB(VCAM signal. CONCLUSIONS: Non-invasive CEU molecular imaging detects the effects of anti-inflammatory treatment on endothelial inflammation in early atherosclerosis. This easily accessible, low-cost technique may be useful in assessing treatment effects in preclinical research and in patients.

  17. Scientists for non-proliferation of nuclear weapons. Transactions of international seminar

    International Nuclear Information System (INIS)

    1994-01-01

    This publication presents the results of the Second International Seminar 'Scientists for Non-Proliferation of Nuclear Weapons'. The Seminar took place from 11 to 14 October 1994 in Nakhabino Country Club near Moscow. More than 60 specialists from Russia, USA, France, Belgium as well as IAEA and CEU took part in the seminar. Problems of cooperation in the field of nuclear materials accounting, control and safeguards, physical protection of nuclear materials, nuclear export regulations and disarmament control are discussed at the seminar

  18. Pontes e rupturas no fabular de Wilson Bueno

    OpenAIRE

    Furquim, Fulvia Maria Giaretta de Almeida

    2008-01-01

    O trabalho tem por objetivo analisar tres obras contemporaneas do escritor paranaense Wilson Bueno (1949- ), que fazem parte de um projeto literario, em que as personagens sao apresentadas de uma visao inicialmente plural, como se apenas uma delas pudesse ser a representacao do todo, para uma optica individualista, caracteristica de um sistema capitalista. Sao elas: Manual de Zoofilia (1991), Jardim Zoologico (1999) e Cachorrros do Ceu (2005). O escritor contemporaneo segue a tradicao dos bes...

  19. Burnup simulations of different fuel grades using the MCNPX Monte Carlo code

    Directory of Open Access Journals (Sweden)

    Asah-Opoku Fiifi

    2014-01-01

    Full Text Available Global energy problems range from the increasing cost of fuel to the unequal distribution of energy resources and the potential climate change resulting from the burning of fossil fuels. A sustainable nuclear energy would augment the current world energy supply and serve as a reliable future energy source. This research focuses on Monte Carlo simulations of pressurized water reactor systems. Three different fuel grades - mixed oxide fuel (MOX, uranium oxide fuel (UOX, and commercially enriched uranium or uranium metal (CEU - are used in this simulation and their impact on the effective multiplication factor (Keff and, hence, criticality and total radioactivity of the reactor core after fuel burnup analyzed. The effect of different clad materials on Keff is also studied. Burnup calculation results indicate a buildup of plutonium isotopes in UOX and CEU, as opposed to a decline in plutonium radioisotopes for MOX fuel burnup time. For MOX fuel, a decrease of 31.9% of the fissile plutonium isotope is observed, while for UOX and CEU, fissile plutonium isotopes increased by 82.3% and 83.8%, respectively. Keff results show zircaloy as a much more effective clad material in comparison to zirconium and stainless steel.

  20. Spain in South Ukraine NPP

    International Nuclear Information System (INIS)

    Ibanez, M.

    1994-01-01

    A Technical Assistance Protocol was signed between the Governments of the GIS and the Commission of the European Union (CEU) on August 2, 1991 and this was the starting point of the TACIS program. In this article, the activities described are those related to the TACIS-92/93/94 on site technical assistance to South Ukraine NPP (SUK NPP). Within the scope of the TACIS 92 Program the CEU and the Ukrainian Authorities agreed a list of projects to be implemented at South Ukraine NPP with the aim to improve the operational safety of the plant. This part of the program is called TACIS 92 on-site activities. The total budget allocated to these projects is a MECU. The European Union ''utility'' selected to lead this program at South Ukraine NPP was UNESA and the first contract to cover our activities was signed in July 1993 between the CEU (Mr. Pablo Benavides) and UNESA (Mr. Pedro Rivero). The projects will be implemented at SUK NPP but according to the contract UNESA is ''The Consultant'' and GOSKOMATON (The Ukrainian Sate Committee on Nuclear Power Utilization) is the ''Recipient Institution''. (Author)

  1. Microtubule disruption induced in vivo by alkylation of beta-tubulin by 1-aryl-3-(2-chloroethyl)ureas, a novel class of soft alkylating agents.

    Science.gov (United States)

    Legault, J; Gaulin, J F; Mounetou, E; Bolduc, S; Lacroix, J; Poyet, P; Gaudreault, R C

    2000-02-15

    We have previously reported that 4-tert-butyl-[3-(2-chloroethyl)ureido] benzene (4-tBCEU), a potent cytotoxic agent, modulates the synthesis of tubulins, suggesting that its cytotoxicity may be mediated through an antimicrotubule mechanism. Indeed, 4-tBCEU and its 4-iso-propyl (4-isopropyl [3-(2-chloroethyl)ureido] benzene) and 4-sec-butyl (4-sec-butyl [3-(2-chloroethyl)ureido] benzene) homologues induced disruption of the cytoskeleton and arrest of the cell cycle in G2 transition and mitosis. To better understand the mechanisms responsible for microtubule disruption by 1-aryl-3-(2-chloroethyl)ureas (CEU), we first examined their cytotoxicity on Chinese hamster ovary cells resistant to vinblastine and colchicine due to the expression of mutated tubulins (CHO-VV 3-2). These cells showed resistance to CEU, e.g., 4-tBCEU having an IC50 of 21.3+/-1.1 microM as compared with an IC50 of 11.6+/-0.7 microM for wild-type cells, suggesting a direct effect of the drugs on tubulins. Western blot analysis confirmed the disruption of microtubules and evidenced the formation of an additional immunoreactive beta-tubulin with an apparent lower molecular weight on SDS polyacrylamide gel. Incubation of MDA-MB-231 cells with [urea-14C]-4-tBCEU revealed the presence of a radioactive protein that coincided with the additional beta-tubulin band, indicating that CEU could covalently bind to the beta-tubulin. The 4-tBCEU-binding site on beta-tubulin was identified by competition of the CEU with colchicine, vinblastine, and iodoacetamide, a specific alkylating agent of sulfhydryl groups of cysteine residues. Colchicine, but not vinblastine, prevented the formation of the additional beta-tubulin band, suggesting that 4-tBCEU alkylates either Cys239 or Cys354 residues near the colchicine-binding site. To determine the cysteine residue alkylated by 4-tBCEU, we incubated the radiolabeled drug with human neuroblastoma cells (SK-N-SH) that overexpress the betaIII-tubulin, an isoform where Cys239

  2. The CFTR Met 470 allele is associated with lower birth rates in fertile men from a population isolate.

    Directory of Open Access Journals (Sweden)

    Gülüm Kosova

    2010-06-01

    Full Text Available Although little is known about the role of the cystic fibrosis transmembrane regulator (CFTR gene in reproductive physiology, numerous variants in this gene have been implicated in etiology of male infertility due to congenital bilateral absence of the vas deferens (CBAVD. Here, we studied the fertility effects of three CBAVD-associated CFTR polymorphisms, the (TGm and polyT repeat polymorphisms in intron 8 and Met470Val in exon 10, in healthy men of European descent. Homozygosity for the Met470 allele was associated with lower birth rates, defined as the number of births per year of marriage (P = 0.0029. The Met470Val locus explained 4.36% of the phenotypic variance in birth rate, and men homozygous for the Met470 allele had 0.56 fewer children on average compared to Val470 carrier men. The derived Val470 allele occurs at high frequencies in non-African populations (allele frequency = 0.51 in HapMap CEU, whereas it is very rare in African population (Fst = 0.43 between HapMap CEU and YRI. In addition, haplotypes bearing Val470 show a lack of genetic diversity and are thus longer than haplotypes bearing Met470 (measured by an integrated haplotype score [iHS] of -1.93 in HapMap CEU. The fraction of SNPs in the HapMap Phase2 data set with more extreme Fst and iHS measures is 0.003, consistent with a selective sweep outside of Africa. The fertility advantage conferred by Val470 relative to Met470 may provide a selective mechanism for these population genetic observations.

  3. Engaging undergraduate students in hadron physics research and instrumentation

    Science.gov (United States)

    Horn, Tanja

    2017-09-01

    Nuclear physics research is fundamental to our understanding of the visible universe and at the same time intertwined with our daily life. Nuclear physics studies the origin and structure of the atomic nuclei in terms of their basic constituents, the quarks and gluons. Atoms and molecules would not exist without underlying quark-gluon interactions, which build nearly all the mass of the visible universe from an assembly of massless gluons and nearly-massless quarks. The study of hadron structure with electromagnetic probes through exclusive and semi-inclusive scattering experiments carried out at the 12 GeV Jefferson Laboratory plays an important role in this effort. In particular, planned precision measurements of pion and kaon form factors and longitudinal-transverse separated deep exclusive pion and kaon electroproduction cross sections to the highest momentum transfers achievable play an important role in understanding hadron structure and masses and provide essential constraints for 3D hadron imaging. While a growing fraction of nuclear physics research is carried out at large international laboratories, individual university research groups play critical roles in the success of that research. These include data analysis projects and the development of state-of-the-art instrumentation demanded by increasingly sophisticated experiments. These efforts are empowered by the creativity of university faculty, staff, postdocs, and provide students with unique hands-on experience. As an example, an aerogel Cherenkov detector enabling strangeness physics research in Hall C at Jefferson Lab was constructed at the Catholic University of America with the help of 16 undergraduate and high school students. The ''Conference Experience for Undergraduates'' (CEU) provides a venue for these students who have conducted research in nuclear physics. This presentation will present the experiences of one of the participants in the first years of the CEU, her current research program

  4. An evaluation of the performance of tag SNPs derived from HapMap in a Caucasian population.

    Directory of Open Access Journals (Sweden)

    Alexandre Montpetit

    2006-03-01

    Full Text Available The Haplotype Map (HapMap project recently generated genotype data for more than 1 million single-nucleotide polymorphisms (SNPs in four population samples. The main application of the data is in the selection of tag single-nucleotide polymorphisms (tSNPs to use in association studies. The usefulness of this selection process needs to be verified in populations outside those used for the HapMap project. In addition, it is not known how well the data represent the general population, as only 90-120 chromosomes were used for each population and since the genotyped SNPs were selected so as to have high frequencies. In this study, we analyzed more than 1,000 individuals from Estonia. The population of this northern European country has been influenced by many different waves of migrations from Europe and Russia. We genotyped 1,536 randomly selected SNPs from two 500-kbp ENCODE regions on Chromosome 2. We observed that the tSNPs selected from the CEPH (Centre d'Etude du Polymorphisme Humain from Utah (CEU HapMap samples (derived from US residents with northern and western European ancestry captured most of the variation in the Estonia sample. (Between 90% and 95% of the SNPs with a minor allele frequency of more than 5% have an r2 of at least 0.8 with one of the CEU tSNPs. Using the reverse approach, tags selected from the Estonia sample could almost equally well describe the CEU sample. Finally, we observed that the sample size, the allelic frequency, and the SNP density in the dataset used to select the tags each have important effects on the tagging performance. Overall, our study supports the use of HapMap data in other Caucasian populations, but the SNP density and the bias towards high-frequency SNPs have to be taken into account when designing association studies.

  5. The regulatory effect of miRNAs is a heritable genetic trait in humans

    Directory of Open Access Journals (Sweden)

    Geeleher Paul

    2012-08-01

    Full Text Available Abstract Background microRNAs (miRNAs have been shown to regulate the expression of a large number of genes and play key roles in many biological processes. Several previous studies have quantified the inhibitory effect of a miRNA indirectly by considering the expression levels of genes that are predicted to be targeted by the miRNA and this approach has been shown to be robust to the choice of prediction algorithm. Given a gene expression dataset, Cheng et al. defined the regulatory effect score (RE-score of a miRNA as the difference in the gene expression rank of targets of the miRNA compared to non-targeted genes. Results Using microarray data from parent-offspring trios from the International HapMap project, we show that the RE-score of most miRNAs is correlated between parents and offspring and, thus, inter-individual variation in RE-score has a genetic component in humans. Indeed, the mean RE-score across miRNAs is correlated between parents and offspring, suggesting genetic differences in the overall efficiency of the miRNA biogenesis pathway between individuals. To explore the genetics of this quantitative trait further, we carried out a genome-wide association study of the mean RE-score separately in two HapMap populations (CEU and YRI. No genome-wide significant associations were discovered; however, a SNP rs17409624, in an intron of DROSHA, was significantly associated with mean RE-score in the CEU population following permutation-based control for multiple testing based on all SNPs mapped to the canonical miRNA biogenesis pathway; of 244 individual miRNA RE-scores assessed in the CEU, 214 were associated (p p = 0.04 with mean RE-score in the YRI population. Interestingly, the same SNP was associated with 17 (8.5% of all expressed miRNA expression levels in the CEU. We also show here that the expression of the targets of most miRNAs is more highly correlated with global changes in miRNA regulatory effect than with the expression of

  6. La juventud universitaria española ante la Defensa Nacional: Encuesta sobre percepción y actitudes

    OpenAIRE

    Olianas, Stefania; Ramirez, J. Martin

    2006-01-01

    El Grupo Complutense de Investigación sobre Neuropsicopedagogía de la Agresión y la Asociación de Diplomados en Altos Estudios de la Defensa Nacional (ADALEDE), con la colaboración de otras universidades madrileñas (Alcalá, Rey Juan Carlos y San Pablo-CEU), han realizado una encuesta entre jóvenes universitarios, con el objetivo de evaluar su percepción, conciencia, opinión y grado de conocimiento que tienen sobre diversos temas relacionados con la Defensa Nacional.

  7. Universos y categorías de análisis del envejecimiento activo en los magacines de radio en España

    OpenAIRE

    Suay Madrid, Amparo

    2016-01-01

    Esta clasificación la hemos creado en la Universidad CEU Cardenal Herrera de Alfara del Patriarca en Valencia, a partir de una investigación centrada en el discurso del envejecimiento activo, en cuanto a calidad de vida, participación social e incremento de la ciudadanía. Para ello, hemos realizado un análisis empírico en los magacines de la radio española desde febrero de 2014 a enero de 2015, contemplando para ello dos muestras de programas. En estas muestras de program...

  8. Actitud de los alumnos de quinto de medicina hacia la salud mental Medicine 5th course student's attitudes towards mental health

    OpenAIRE

    Inmaculada Gilaberte; Inmaculada Failde; Alejandro Salazar; Luis Caballero

    2012-01-01

    Introducción. El estigma social que persiste hacia la enfermedad mental puede estar presente en los estudiantes de medicina e impactar en la forma de considerar ésta y la psiquiatría como especialidad. Objetivo. Investigar la actitud hacia la enfermedad mental y la psiquiatría en una muestra de estudiantes. Sujetos y métodos. Se realizó una encuesta estructurada, voluntaria y anónima a los 27 estudiantes de quinto curso del grado de medicina de la Universidad San Pablo-CEU de Madrid. La encue...

  9. Actitud de los alumnos de 5º de medicina hacia la Salud Mental.

    OpenAIRE

    Gilaberte, Inmaculada; Failde, Inmaculada; Salazar, Alejandro; Caballero, Luis

    2012-01-01

    Introducción. El estigma social que persiste hacia la enfermedad mental puede estar presente en los estudiantes de medicina e impactar en la forma de considerar ésta y la psiquiatría como especialidad. Objetivo. Investigar la actitud hacia la enfermedad mental y la psiquiatría en una muestra de estudiantes. Sujetos y métodos. Se realizó una encuesta estructurada, voluntaria y anónima a los 27 estudiantes de quinto curso del grado de medicina de la Universidad San Pablo-CEU de Madr...

  10. Spanish Pacification Campaigns in Morocco (1909-1927): Developing Indigenous Forces in Counterinsurgency

    Science.gov (United States)

    2017-05-25

    español en Marruecos (1898-1928): análisis de factores que confluyen en un desastre militar, Annual” (PhD thesis, Universidad Complutense de Madrid...Echevarría, “Intervencionismo español en Marruecos (1898-1928): análisis de factores que confluyen en un desastre militar, Annual” (PhD thesis, Universidad ...Oficial José Enrique Varela Iglesias” (PhD thesis, Universidad Cardenal Herrera-CEU de Valencia, 2012), 395. 123 Serrano Sáenz de Tejada, De la

  11. Modernism: Representations of National Culture

    OpenAIRE

    Ersoy, Ahmet; Górny, Maciej; Kechriotis, Vangelis; Ady, Endre; Bedirxan, Celadet Alî; Beöthy, Zsolt; Bobrzyński, Michał; Boilǎ, Romul; Caragiale, Ion Luca; Fikret, Tevfik; Fishta, Gjergj; Gombrowicz, Witold; Górski, Artur; Gusti, Dimitrie; Hašek, Jaroslav

    2012-01-01

    This is the second part of the third volume of the four-volume series, a daring project of CEU Press, presenting the most important texts that triggered and shaped the processes of nation-building in the many countries of Central and Southeast Europe. The aim is to confront ‘mainstream’ and seemingly successful national discourses with each other, thus creating a space for analyzing those narratives of identity which became institutionalized as “national canons.” After the volumes focousing ...

  12. Towards Energy Efficiency: Forecasting Indoor Temperature via Multivariate Analysis

    Directory of Open Access Journals (Sweden)

    Juan Pardo

    2013-09-01

    Full Text Available The small medium large system (SMLsystem is a house built at the Universidad CEU Cardenal Herrera (CEU-UCH for participation in the Solar Decathlon 2013 competition. Several technologies have been integrated to reduce power consumption. One of these is a forecasting system based on artificial neural networks (ANNs, which is able to predict indoor temperature in the near future using captured data by a complex monitoring system as the input. A study of the impact on forecasting performance of different covariate combinations is presented in this paper. Additionally, a comparison of ANNs with the standard statistical forecasting methods is shown. The research in this paper has been focused on forecasting the indoor temperature of a house, as it is directly related to HVAC—heating, ventilation and air conditioning—system consumption. HVAC systems at the SMLsystem house represent 53:89% of the overall power consumption. The energy used to maintain temperature was measured to be 30%–38:9% of the energy needed to lower it. Hence, these forecasting measures allow the house to adapt itself to future temperature conditions by using home automation in an energy-efficient manner. Experimental results show a high forecasting accuracy and therefore, they might be used to efficiently control an HVAC system.

  13. Genetic polymorphisms of pharmacogenomic VIP variants in the Kyrgyz population from northwest China.

    Science.gov (United States)

    Yunus, Zulfiya; Liu, Lijun; Wang, Hong; Zhang, Le; Li, Xiaolan; Geng, Tingting; Kang, Longli; Jin, Tianbo; Chen, Chao

    2013-10-15

    Pharmacogenomic variant information is well known for major human populations; however, this information is less commonly studied in minorities. In the present study, we genotyped 85 very important pharmacogenetic (VIP) variants (selected from the PharmGKB database) in the Kyrgyz population and compared our data with other four major human populations including Han Chinese in Beijing, China (CHB), the Japanese in Tokyo, Japan (JPT), a northern and western Europe population (CEU), and the Yoruba in Ibadan, Nigeria (YRI). There were 13, 12 and 16 of the selected VIP variant genotype frequencies in the Kyrgyz which differed from those of the CHB, JPT and CEU, respectively (p<0.005). In the YRI, there were 32 different variants, compared to the Kyrgyz (p<0.005). Genotype frequencies of ADH1B, AHR, CYP3A5, PTGS2, VDR, and VKORC1 in the Kyrgyz differed widely from those in the four populations. Haplotype analyses also showed differences among the Kyrgyz and the other four populations. Our results complement the information provided by the database of pharmacogenomics on Kyrgyz. We provide a theoretical basis for safer drug administration and individualized treatment plans for the Kyrgyz. We also provide a template for the study of pharmacogenomics in various ethnic minority groups in China. © 2013 Elsevier B.V. All rights reserved.

  14. Detection of seven virulence and toxin genes of Campylobacter jejuni isolates from Danish turkeys by PCR and cytolethal distending toxin production of the isolates

    DEFF Research Database (Denmark)

    Bang, Dang Duong; Borck, Birgitte; Nielsen, Eva Møller

    2004-01-01

    A total of 117 Campylobacter jejuni isolates from Danish turkeys were tested for the presence of seven virulence and toxin genes by PCR. One hundred seventeen (100%) isolates were positive for flaA, cadF, and ceuE gene primers. One hundred three (88%) isolates were positive for cdt gene cluster PCR.......7%) in Colon 205 assays, and 109 (93.2%) in chicken embryo cell assays. The CDT titers were determined in Vero cell assays. Of 117 isolates, 50 (42.7%) produced a CDT titer of 1:100, 29 (24.8%) of 1:50, and 27 (23%) of 1:5 to 1:10; 8 (6.8%) produced a CDT titer at undiluted supernatants and 3 (2.6%) produced...

  15. Editorial 1: Presente y futuro de la Comunicación Estratégica. Estudios de caso / The Present and Future of Strategic Communication. Case Studies

    Directory of Open Access Journals (Sweden)

    Victoria Tur-Viñes

    2014-12-01

    Full Text Available Con este número, concluimos el quinto año de edición continuada de Revista Mediterránea de Comunicación. La comunicación estratégica es el tema monográfico del dossier, coordinado por la profesora Catedrática Dra. María Isabel de Salas Nestares de la Universidad CEU Cardenal Herrera de Valencia (España. Ha contado con siete colaboraciones, una de ellas internacional. La complementariedad de los textos permite disfrutar de una panorámica actual del estado de la comunicación estratégica en el seno de las organizaciones sin dejar de lado los obstáculos superados y los retos futuros. Encuentran detalles y comentarios sobre su contenido en el editorial de la coordinadora.

  16. Correlation between genetic and geographic structure in Europe

    DEFF Research Database (Denmark)

    Lao, Oscar; Lu, Timothy T; Nothnagel, Michael

    2008-01-01

    geographic but narrow genomic coverage [1, 2], or vice versa [3-6]. We therefore investigated Affymetrix GeneChip 500K genotype data from 2,514 individuals belonging to 23 different subpopulations, widely spread over Europe. Although we found only a low level of genetic differentiation between subpopulations......, the existing differences were characterized by a strong continent-wide correlation between geographic and genetic distance. Furthermore, mean heterozygosity was larger, and mean linkage disequilibrium smaller, in southern as compared to northern Europe. Both parameters clearly showed a clinal distribution...... Europe. By including the widely used CEPH from Utah (CEU) samples into our analysis, we could show that these individuals represent northern and western Europeans reasonably well, thereby confirming their assumed regional ancestry....

  17. Avant-Propos

    OpenAIRE

    Ruppert, Helmut

    2013-01-01

    C’est à l’instigation de mon professeur, M. Eugen Wirth, que je me suis lancé dans ce travail. Lors d’un premier voyage au printemps 1966, il me fit ouvrir les yeux sur quelques-uns des importants problèmes de la géographie sociale et économique du Proche-Orient. Lors de voyages ultérieurs passés à parcourir et à arpenter avec moi d’autres villes orientales, le professeur Wirth m’a permis de comparer pour la ville de Beyrouth, grâce à une vue générale, les éléments proprement orientaux et ceu...

  18. Total mercury in muscles and liver of Mugil spp. from three coastal lagoons of NW Mexico: concentrations and risk assessment.

    Science.gov (United States)

    Delgado-Alvarez, C G; Frías-Espericueta, M G; Ruelas-Inzunza, J; Becerra-Álvarez, M J; Osuna-Martínez, C C; Aguilar-Juárez, M; Osuna-López, J I; Escobar-Sánchez, O; Voltolina, D

    2017-07-01

    Total mercury (Hg) concentrations were determined by atomic absorption spectrophotometry in muscles and liver of composite samples of Mugil cephalus and M. curema collected during November 2013 and in January, April, and July 2014 from the coastal lagoons Altata-Ensenada del Pabellón (AEP), Ceuta (CEU), and Teacapán-Agua Brava (TAG) of Sinaloa State. The mean Hg contents and information on local consumption were used to assess the possible risk caused by fish ingestion. Mean total mercury levels in the muscles ranged from 0.11 to 0.39 μg/g, while the range for liver was 0.12-3.91 μg/g. The mean Hg content of the liver was significantly (p mercury calculated for the younger age classes of one fishing community were >1, indicating a possible risk for some fishing communities of the Mexican Pacific coast.

  19. Résister : les poèmes de guerre de Charles Camproux

    OpenAIRE

    Lassaque, Aurélia

    2014-01-01

    Charles Camproux, né à Marseille en 1908, fut publiquement reconnu comme poète suite à la parution des Poemas sens poesia. Ce premier recueil portant sur la captivité, composé « sot lo ceu d’Alemanha », fut publié peu après le retour de Camproux en France, par les soins d’Ismaël Girard à la SEO en 1942. Camproux composa au cours des deux années suivantes un ensemble de poèmes réunis tardivement sous le titre de Poëmas de Resistència. Contrairement à L’an quaranta e tres et au Bestiari, écrits...

  20. Life-Cycle Assessment of Prototype Unit of Emergency Housing. The search for the zero impact

    Directory of Open Access Journals (Sweden)

    J. M. Ros García

    2017-09-01

    Full Text Available Prototype Unit of Emergency Housing (PUEH is the result of the Applied Research Project VEM (Military Emergency Housing developed in collaboration with Escuela Politécnica Superior (Universidad CEU and the company Air-bus Defense & Space. It is designed as a modular and industrialized unit of basic habitability, with programmed and expandable growth, designed to provide shelter and protection in environments of humanitarian crises or contingencies of social vulnerability in order to ensure sustainable habitat for emergencies.The influence of the construction processes and materials involved in the manufacture of this PUEH have on the environment, analyzed using the methodology of life-cycle assessment (LCA, considered especially critical recycling the mate-rials used. Thus, in order to reduce the environmental impact environmental, each of the component parts of the developed prototype unit are quantified, evaluating the benefits resulting from the methodology DfMA (Design for Manufacturing and Assembly.

  1. Pharmacogenetic landscape of Metabolic Syndrome components drug response in Tunisia and comparison with worldwide populations.

    Science.gov (United States)

    Jmel, Haifa; Romdhane, Lilia; Ben Halima, Yosra; Hechmi, Meriem; Naouali, Chokri; Dallali, Hamza; Hamdi, Yosr; Shan, Jingxuan; Abid, Abdelmajid; Jamoussi, Henda; Trabelsi, Sameh; Chouchane, Lotfi; Luiselli, Donata; Abdelhak, Sonia; Kefi, Rym

    2018-01-01

    Genetic variation is an important determinant affecting either drug response or susceptibility to adverse drug reactions. Several studies have highlighted the importance of ethnicity in influencing drug response variability that should be considered during drug development. Our objective is to characterize the genetic variability of some pharmacogenes involved in the response to drugs used for the treatment of Metabolic Syndrome (MetS) in Tunisia and to compare our results to the worldwide populations. A set of 135 Tunisians was genotyped using the Affymetrix Chip 6.0 genotyping array. Variants located in 24 Very Important Pharmacogenes (VIP) involved in MetS drug response were extracted from the genotyping data. Analysis of variant distribution in Tunisian population compared to 20 worldwide populations publicly available was performed using R software packages. Common variants between Tunisians and the 20 investigated populations were extracted from genotyping data. Multidimensional screening showed that Tunisian population is clustered with North African and European populations. The greatest divergence was observed with the African and Asian population. In addition, we performed Inter-ethnic comparison based on the genotype frequencies of five VIP biomarkers. The genotype frequencies of the biomarkers rs3846662, rs1045642, rs7294 and rs12255372 located respectively in HMGCR, ABCB1, VKORC1 and TCF7L2 are similar between Tunisian, Tuscan (TSI) and European (CEU). The genotype frequency of the variant rs776746 located in CYP3A5 gene is similar between Tunisian and African populations and different from CEU and TSI. The present study shows that the genetic make up of the Tunisian population is relatively complex in regard to pharmacogenes and reflects previous historical events. It is important to consider this ethnic difference in drug prescription in order to optimize drug response to avoid serious adverse drug reactions. Taking into account similarities with

  2. Pharmacogenetic landscape of Metabolic Syndrome components drug response in Tunisia and comparison with worldwide populations

    Science.gov (United States)

    Jmel, Haifa; Romdhane, Lilia; Ben Halima, Yosra; Hechmi, Meriem; Naouali, Chokri; Dallali, Hamza; Hamdi, Yosr; Shan, Jingxuan; Abid, Abdelmajid; Jamoussi, Henda; Trabelsi, Sameh; Chouchane, Lotfi; Luiselli, Donata; Abdelhak, Sonia

    2018-01-01

    Genetic variation is an important determinant affecting either drug response or susceptibility to adverse drug reactions. Several studies have highlighted the importance of ethnicity in influencing drug response variability that should be considered during drug development. Our objective is to characterize the genetic variability of some pharmacogenes involved in the response to drugs used for the treatment of Metabolic Syndrome (MetS) in Tunisia and to compare our results to the worldwide populations. A set of 135 Tunisians was genotyped using the Affymetrix Chip 6.0 genotyping array. Variants located in 24 Very Important Pharmacogenes (VIP) involved in MetS drug response were extracted from the genotyping data. Analysis of variant distribution in Tunisian population compared to 20 worldwide populations publicly available was performed using R software packages. Common variants between Tunisians and the 20 investigated populations were extracted from genotyping data. Multidimensional screening showed that Tunisian population is clustered with North African and European populations. The greatest divergence was observed with the African and Asian population. In addition, we performed Inter-ethnic comparison based on the genotype frequencies of five VIP biomarkers. The genotype frequencies of the biomarkers rs3846662, rs1045642, rs7294 and rs12255372 located respectively in HMGCR, ABCB1, VKORC1 and TCF7L2 are similar between Tunisian, Tuscan (TSI) and European (CEU). The genotype frequency of the variant rs776746 located in CYP3A5 gene is similar between Tunisian and African populations and different from CEU and TSI. The present study shows that the genetic make up of the Tunisian population is relatively complex in regard to pharmacogenes and reflects previous historical events. It is important to consider this ethnic difference in drug prescription in order to optimize drug response to avoid serious adverse drug reactions. Taking into account similarities with

  3. Importance of pharmaceutical laboratory compliance with international standard requirements in respect of raising their competitiveness

    Directory of Open Access Journals (Sweden)

    Božanić Vojislav N.

    2009-01-01

    Full Text Available Current Good Manufacturing Practice (cGMP being a legal regulation in developed countries will become a legal regulation in Republic of Serbia starting with March 2010. In this paper comparative analysis between requirements of standard ISO/IEC 17025 and requirements of cEU GMP is shown. Considering the fact that in Republic of Serbia no pharmaceutical industry laboratory has been accredited according to requirements of ISO/IEC 17025, while keeping in mind that more than 90% of these laboratories have not fulfilled cEU GMP requirements, this paper aimed at pointing to the possibility of fulfilling both of mentioned requirements at the same time, which would open the way to different types of interlaboratory cooperation for pharmaceutical quality control laboratories and contribute to improving competitiveness of pharmaceutical companies. Accreditation, especially in the case of pharmaceutical quality control laboratories, is important because it guaranties the level of organizational and technical competency. It could easily be said that accreditation is becoming a must in quality control of products in order for the organization to be able to gain a leading role in the global market. Both accreditation and cGMP show the organization's commitment to having products of highest quality level. Considering the above mentioned facts, it is of greatest advantage for pharmaceutical quality control laboratories to fulfill both requirements of ISO/IEC 17025 and cGMP and reach total compliance. The aim of doing this lies in an easier acceptance of pharmaceutical products in different markets, overcoming technical barriers and affirmation of quality as key factor in reaching competitiveness, while keeping in mind the importance of strategic and competitive positioning in the global market.

  4. Antimicrobial Susceptibility and Genotypic Characteristic of Campylobacter spp. Isolates from Free-Living Birds in Poland.

    Science.gov (United States)

    Krawiec, Marta; Woźniak-Biel, Anna; Bednarski, Michał; Wieliczko, Alina

    2017-11-01

    Campylobacter spp. is the most commonly reported, bacterial cause of human foodborne infection worldwide. Commercial poultry and free-living birds are natural reservoirs of three particular species: Campylobacter jejuni, Campylobacter coli, and Campylobacter lari. The aim of this study was to determine the genotypic characteristics and antibiotic susceptibility of 43 Campylobacter strains, obtained from free-living birds, in Poland. In total, 700 birds were examined. The strains were isolated from 43 birds (6.14%) from the feces of 7 wild bird species: Mallard ducks Anas platyrhynchos (29 positive/121 tested), great cormorants Phalacrocorax carbo (5/77), velvet scoters Melanitta fusca (4/30), tawny owls Strix aluco (2/5), common buzzard Buteo buteo (1/3), rook Corvus frugilegus (1/6), and Eurasian tree sparrow Passer montanus (1/30). Thirty-eight (88.37%) of obtained strains belonged to C. jejuni and five (11.63%) to C. coli. Other 428 examined birds from different bird species were Campylobacter negative. The antimicrobial susceptibility to nine antimicrobials was also studied in investigated isolates of Campylobacter spp. Sixteen of the examined strains (37.21% of all positive samples) showed susceptibility to all of the nine antimicrobials. Moreover, the prevalence of selected virulence genes, such as flaA, cadF, ceuE, virB11, cdtA, cdtB, and cdtC were all analyzed. The virulence gene that was found most frequently in total number of Campylobacter strains was ceuE (72.10%) and other genes, such as flaA, cadF, cdtA, cdtB, and cdtC, were found in over 60% of all examined strains. Variable antimicrobial susceptibility and the presence of different virulence genes of examined strains, isolated from free-living birds, suggest that special attention should be given to wild birds and any potential approaches to the control of antibiotic-resistant Campylobacter should be discussed.

  5. Grandes retos y aplicación de herramientas participativas en la planificación de la ciudad informal. El caso de Makeni, Sierra Leona

    Directory of Open Access Journals (Sweden)

    Luis Perea Moreno

    2018-05-01

    Full Text Available El siguiente artículo indaga sobre la definición de modelos de planificación y herramientas de gestión para ciudades con crecimientos expansivos y amplios sectores informales, utilizando como caso de estudio la experiencia desarrollada en la ciudad de Makeni, Sierra Leona. En un proceso de varios años que continua vivo, la Escuela de Arquitectura de la Universidad San Pablo CEU (USP-CEU ha impulsado acciones de planeamiento urbano participativo, aportando experiencia técnica desde la colaboración con la Universidad de Makeni y con el Ayuntamiento de la ciudad. Avanzando en el conocimiento paulatino de una realidad específica llena de retos, se han abordado propuestas urbanas en dos líneas fundamentales. Por un lado, mediante el desarrollo de un Plan Espacial Estratégico para Makeni, destinado a la generación de propuestas para la ciudad y su territorio; y, por otro, con la implementación del Programa de Mejoramiento de Barrios, centrado en la ciudad existente. En relación a los resultados, en el territorio de Makeni, destacan el desarrollo de las capacidades formativas de los alumnos de la universidad local, la generación de metodologías e instrumentos de planeamiento básicos, el reforzamiento de los cuadros administrativos municipales, junto a la implicación y concienciación de la población local. A nivel global, hay que destacar la definición de un marco coherente y sistemático, factible de ser replicado en otras realidades urbanas con similares características. Un aporte que resulta especialmente válido en aquellos contextos urbanos desfavorecidos que, en sus procesos de crecimiento, afrontan el mayor reto futuro vinculado a la habitabilidad a nivel global.

  6. Genetic admixture and population substructure in Guanacaste Costa Rica.

    Directory of Open Access Journals (Sweden)

    Zhaoming Wang

    2010-10-01

    Full Text Available The population of Costa Rica (CR represents an admixture of major continental populations. An investigation of the CR population structure would provide an important foundation for mapping genetic variants underlying common diseases and traits. We conducted an analysis of 1,301 women from the Guanacaste region of CR using 27,904 single nucleotide polymorphisms (SNPs genotyped on a custom Illumina InfiniumII iSelect chip. The program STRUCTURE was used to compare the CR Guanacaste sample with four continental reference samples, including HapMap Europeans (CEU, East Asians (JPT+CHB, West African Yoruba (YRI, as well as Native Americans (NA from the Illumina iControl database. Our results show that the CR Guanacaste sample comprises a three-way admixture estimated to be 43% European, 38% Native American and 15% West African. An estimated 4% residual Asian ancestry may be within the error range. Results from principal components analysis reveal a correlation between genetic and geographic distance. The magnitude of linkage disequilibrium (LD measured by the number of tagging SNPs required to cover the same region in the genome in the CR Guanacaste sample appeared to be weaker than that observed in CEU, JPT+CHB and NA reference samples but stronger than that of the HapMap YRI sample. Based on the clustering pattern observed in both STRUCTURE and principal components analysis, two subpopulations were identified that differ by approximately 20% in LD block size averaged over all LD blocks identified by Haploview. We also show in a simulated association study conducted within the two subpopulations, that the failure to account for population stratification (PS could lead to a noticeable inflation in the false positive rate. However, we further demonstrate that existing PS adjustment approaches can reduce the inflation to an acceptable level for gene discovery.

  7. Characterization of CTX-M-140, a Variant of CTX-M-14 Extended-Spectrum β-Lactamase with Decreased Cephalosporin Hydrolytic Activity, from Cephalosporin-Resistant Proteus mirabilis.

    Science.gov (United States)

    Tian, Guo-Bao; Jiang, Yi-Qi; Huang, Ying-Min; Qin, Yun; Feng, Lian-Qiang; Zhang, Xue-Fei; Li, Hong-Yu; Zhong, Lan-Lan; Zeng, Kun-Jiao; Patil, Sandip; Xing, Yong; Huang, Xi

    2016-10-01

    CTX-M-140, a novel CTX-M-type extended-spectrum β-lactamase (ESBL), was identified in cephalosporin-resistant clinical isolates of Proteus mirabilis CTX-M-140 contained an alanine-to-threonine substitution at position 109 compared to its putative progenitor, CTX-M-14. When it was expressed in an Escherichia coli isogenic background, CTX-M-140 conferred 4- to 32-fold lower MICs of cephalosporins than those with CTX-M-14, indicating that the phenotype was attributable to this single substitution. For four mutants of CTX-M-14 that were constructed by site-directed mutagenesis (A109E, A109D, A109K, and A109R mutants), MICs of cephalosporins were similar to those for the E. coli host strain, which suggested that the alanine at position 109 was essential for cephalosporin hydrolysis. The kinetic properties of native CTX-M-14 and CTX-M-140 were consistent with the MICs for the E. coli clones. Compared with that of CTX-M-14, a lower hydrolytic activity against cephalosporins was observed for CTX-M-140. blaCTX-M-140 is located on the chromosome as determined by I-CeuI pulsed-field gel electrophoresis (I-CeuI-PFGE) and Southern hybridization. The genetic environment surrounding blaCTX-M-140 is identical to the sequence found in different plasmids with blaCTX-M-9-group genes among the Enterobacteriaceae Genome sequencing and analysis showed that P. mirabilis strains with blaCTX-M-140 have a genome size of ∼4 Mbp, with a GC content of 38.7% and 23 putative antibiotic resistance genes. Our results indicate that alanine at position 109 is critical for the hydrolytic activity of CTX-M-14 against oxyimino-cephalosporins. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  8. A genome-wide association study of serum uric acid in African Americans

    Directory of Open Access Journals (Sweden)

    Gerry Norman P

    2011-02-01

    Full Text Available Abstract Background Uric acid is the primary byproduct of purine metabolism. Hyperuricemia is associated with body mass index (BMI, sex, and multiple complex diseases including gout, hypertension (HTN, renal disease, and type 2 diabetes (T2D. Multiple genome-wide association studies (GWAS in individuals of European ancestry (EA have reported associations between serum uric acid levels (SUAL and specific genomic loci. The purposes of this study were: 1 to replicate major signals reported in EA populations; and 2 to use the weak LD pattern in African ancestry population to better localize (fine-map reported loci and 3 to explore the identification of novel findings cognizant of the moderate sample size. Methods African American (AA participants (n = 1,017 from the Howard University Family Study were included in this study. Genotyping was performed using the Affymetrix® Genome-wide Human SNP Array 6.0. Imputation was performed using MACH and the HapMap reference panels for CEU and YRI. A total of 2,400,542 single nucleotide polymorphisms (SNPs were assessed for association with serum uric acid under the additive genetic model with adjustment for age, sex, BMI, glomerular filtration rate, HTN, T2D, and the top two principal components identified in the assessment of admixture and population stratification. Results Four variants in the gene SLC2A9 achieved genome-wide significance for association with SUAL (p-values ranging from 8.88 × 10-9 to 1.38 × 10-9. Fine-mapping of the SLC2A9 signals identified a 263 kb interval of linkage disequilibrium in the HapMap CEU sample. This interval was reduced to 37 kb in our AA and the HapMap YRI samples. Conclusions The most strongly associated locus for SUAL in EA populations was also the most strongly associated locus in this AA sample. This finding provides evidence for the role of SLC2A9 in uric acid metabolism across human populations. Additionally, our findings demonstrate the utility of following-up EA

  9. Calendar

    Science.gov (United States)

    2004-06-01

    JULY 2004 2nd World Congress of the Game Theory Society, Faculty of Luminy, Marseille, France 5-9 July 2004 Europa Organisation (europa@europa-organisation.com), +33 5 34 45 26 45, www.gts2004.org Budapest Workshop on Behavioral Economics, Central European University, Budapest, Hungary 5-10 July 2004 Eva Dotzi (behavecon@ceu.hu), www.iza.org/en/calls_conferences/CallCEU_04.pdf FDA'04. 1st IFAC Workshop on Fractional Differentiation and its Applications, Bordeaux, France 19-20 July 2004 IFAC secretariat (fda04@lap.u-bordeaux1.fr), www.lap.u-bordeaux.fr/fda04/ Bachelier Finance Society Third World Congress, InterContinental Hotel, Chicago, IL, USA 21-24 July 2004 bfs2004@uic.edu, www.uic.edu/orgs/bachelier/ BS/IMS 2004. 6th World Congress of the Bernoulli Society for Mathematical Statistics and Probability, Barcelona, Spain 26-31 July 2004 wc2004@pacifico-meetings.com, +34 93 402 13 85, www.imub.ub.es/events/wc2004 AUGUST 2004 Summer School in Econometrics. The Cointegrated VAR Model: Econometric Methodology and Macroeconomic Applications, Institute of Economics, University of Copenhagen, Denmark 2-22 August 2004 Summerschool@econ.ku.dk, www.econ.ku.dk/summerschool SEPTEMBER 2004 First Bonzenfreies Colloquium on Market Dynamics and Quantitative Economics, Alessandria, Palazzo Borsalino, Italy 9-10 September 2004 colloquium@unipmn.it, www.mfn.unipmn.it/~colloqui/ Risk Analysis 2004. 4th International Conference on Computer Simulation in Risk Analysis and Hazard Mitigation, Aldemar Paradise Royal Mare Hotel, Rhodes, Greece 27-29 September 2004 enquiries@wessex.ac.uk, +44 (0)238 029 3223, www.wessex.ac.uk/conferences/2004/risk04/ OCTOBER 2004 IRC Hedge 2004, InterContinental Hotel, London, UK 10, 11 October 2004 enquiries@irc-conferences.com, www.irc-conferences.com/show_conference.php?id=10 NOVEMBER 2004 IRC DICE 2004, InterContinental Hotel, London, UK 22, 23 November 2004 enquiries@irc-conferences.com, www.irc-conferences.com/show_conference.php?id=13 DECEMBER 2004

  10. Variabilidad del patrón respiratorio durante la carga elástica inspiratoria Variability of breathing pattern during inspiratory elastic load

    Directory of Open Access Journals (Sweden)

    Carlos E. D´Negri

    2009-06-01

    Full Text Available La ventilación pulmonar en humanos tiene una variabilidad respiración a respiración no lineal, compleja y caótica. El objetivo del trabajo fue: caracterizar la variabilidad del patrón respiratorio en perros (n: 8 anestesiados respirando bajo carga elástica umbral (CEU inspiratoria (7 a 50 cm H2O. Con el flujo, presión traqueal y esofágica, se analizaron: tiempo inspiratorio (Ti, ritmo [tiempo espiratorio (Te; tiempo total (Ttot, y Ti/Ttot] e impulso central (Vt/Ti, variables relacionadas [volumen corriente (Vt y ventilación pulmonar (Ve]. Se determinaron: variabilidad grosera (varianzas, oscilaciones de baja frecuencia (análisis espectral y memoria a corto plazo (análisis de autocorrelación. La CEU produjo disminución de la varianza de medias en Te, Ttot, Vt y Vt/Ti (p In humans, lung ventilation exhibits breath-to-breath variability and dynamics that are nonlinear, complex and chaotic. Our objective was to characterize the breathing pattern variational activity in anesthetized dogs (n: 8 breathing through threshold inspiratory elastic load (7 to 50 cm H2O. Starting from flow signal and tracheal and esophageal pressures, we analyzed inspiratory time (Ti, timing (expiratory time, Te; total time, Ttot; and Ti/Ttot and central drive (Vt/Ti and variables related to it (tidal volume, Vt and pulmonary ventilation, Ve. We measured gross variability (variances, low frequency oscillations (spectral analysis, and short term memory (autocorrelation analysis. Loading decreased variance of the mean values of Te, Ttot, Vt and Vt/Ti (p < 0.05; the mean of variances for Ti/Ttot increased (p < 0.005 while it decreased for Vt and Vt/Ti (p < 0.05. In general, percent of data recordings with low frequency oscillations (OB% decreased (p < 0.02. During heavy load, timing parameters percent of data recordings with autocorrelations (AU% did not change, but Vt and its related parameters decreased their AU% (p < 0.005. There was a positive correlation (r: 0

  11. A panel of 74 AISNPs: Improved ancestry inference within Eastern Asia.

    Science.gov (United States)

    Li, Cai-Xia; Pakstis, Andrew J; Jiang, Li; Wei, Yi-Liang; Sun, Qi-Fan; Wu, Hong; Bulbul, Ozlem; Wang, Ping; Kang, Long-Li; Kidd, Judith R; Kidd, Kenneth K

    2016-07-01

    Many ancestry informative SNP (AISNP) panels have been published. Ancestry resolution in them varies from three to eight continental clusters of populations depending on the panel used. However, none of these panels differentiates well among East Asian populations. To meet this need, we have developed a 74 AISNP panel after analyzing a much larger number of SNPs for Fst and allele frequency differences between two geographically close population groups within East Asia. The 74 AISNP panel can now distinguish at least 10 biogeographic groups of populations globally: Sub-Saharan Africa, North Africa, Europe, Southwest Asia, South Asia, North Asia, East Asia, Southeast Asia, Pacific and Americas. Compared with our previous 55-AISNP panel, Southeast Asia and North Asia are two newly assignable clusters. For individual ancestry assignment, the likelihood ratio and ancestry components were analyzed on a different set of 500 test individuals from 11 populations. All individuals from five of the test populations - Yoruba (YRI), European (CEU), Han Chinese in Henan (CHNH), Rondonian Surui (SUR) and Ticuna (TIC) - were assigned to their appropriate geographical regions unambiguously. For the other test populations, most of the individuals were assigned to their self-identified geographical regions with a certain degree of overlap with adjacent populations. These alternative ancestry components for each individual thus help give a clearer picture of the possible group origins of the individual. We have demonstrated that the new AISNP panel can achieve a deeper resolution of global ancestry. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  12. A Rapid and Simple Real-Time PCR Assay for Detecting Foodborne Pathogenic Bacteria in Human Feces.

    Science.gov (United States)

    Hanabara, Yutaro; Ueda, Yutaka

    2016-11-22

    A rapid, simple method for detecting foodborne pathogenic bacteria in human feces is greatly needed. Here, we examined the efficacy of a method that employs a combination of a commercial PCR master mix, which is insensitive to PCR inhibitors, and a DNA extraction method which used sodium dodecyl benzene sulfonate (SDBS), and Tween 20 to counteract the inhibitory effects of SDBS on the PCR assay. This method could detect the target genes (stx1 and stx2 of enterohemorrhagic Escherichia coli, invA of Salmonella Enteritidis, tdh of Vibrio parahaemolyticus, gyrA of Campylobacter jejuni, ceuE of Campylobacter coli, SEA of Staphylococcus aureus, ces of Bacillus cereus, and cpe of Clostridium perfringens) in a fecal suspension containing 1.0 × 10 1 to 1.0 × 10 3 CFU/ml. Furthermore, the assay was neither inhibited nor influenced by individual differences among the fecal samples of 10 subjects or fecal concentration (40-160 mg/ml in the fecal suspension). When we attempted to detect the genes of pathogenic bacteria in 4 actual clinical cases, we found that this method was more sensitive than standard culture method. These results showed that this assay is a rapid, simple detection method for foodborne pathogenic bacteria in human feces.

  13. Intravitreal pegaptanib for refractory macular edema secondary to retinal vein occlusion

    Directory of Open Access Journals (Sweden)

    Udaondo P

    2011-07-01

    Full Text Available Patricia Udaondo1,2, Salvador Garcia-Delpech1,3, David Salom1,3, Maria Garcia-Pous1,3, Manuel Diaz-Llopis1,31Nuevo Hospital Universitario y Politecnico La Fe, Valencia, Spain; 2University Cardenal Herrera CEU, Valencia, Spain; 3Faculty of Medicine, University of Valencia, Valencia, SpainPurpose: To assess the efficacy of intravitreal Pegaptanib sodium (Macugen® injection in the management of refractory macular edema secondary to branch retinal vein occlusion.Methods: This is a prospective, nonrandomized, interventional case series. Five eyes of five patients with macular edema refractory to either bevacizumab or triamcinolone were treated with intravitreal injection of Pegaptanib sodium.Results: After three months follow-up, both visual acuity and macular edema, measured by optical coherence tomography and fluorescence angiography, dramatically improved.Conclusion: Pegaptanib sodium is a safe and efficacy treatment for macular edema secondary to branch retinal vein occlusion.Keywords: Macugen®, BRVO, BCVA, pegaptanib sodium

  14. Q fever: a new ocular manifestation

    Directory of Open Access Journals (Sweden)

    Udaondo P

    2011-09-01

    Full Text Available P Udaondo1,3, S Garcia-Delpech1,2, D Salom1,2, M Garcia-Pous1, M Diaz-Llopis1,21Department of Ophthalmology, Nuevo Hospital Universitario y Politecnico La Fe, Valencia, Spain; 2Faculty of Medicine, Universitat de València, Valencia, Spain; 3Universidad Cardenal Herrera CEU, Valencia, SpainAbstract: Q Fever is a zoonosis caused by Coxiella burnetii. Ocular manifestations are rare in this infection. We describe the case of a man complaining of an intense retro-orbital headache, fever, arthralgia, and bilateral loss of vision, who showed an anterior uveitis accompanied by exudative bilateral inferior retinal detachment and optic disk edema. At the beginning, a Vogt–Koyanagi–Harada (VKH syndrome was suspected, but the patient was diagnosed with Q fever and treatment with doxycycline was initiated, with complete resolution after 2 weeks. We wondered if Q fever could unleash VKH syndrome or simulate a VKH syndrome by a similar immunological process.Keywords: Q fever, Vogt–Koyanagi–Harada syndrome, panuveitis, exudative retinal detachment

  15. SO4--SO3- radical pair formation in Ce doped and Ce, U co-doped K3Na(SO4)2: EPR evidence and its role in TSL

    International Nuclear Information System (INIS)

    Natarajan, V.; Seshagiri, T.K.; Kadam, R.M.; Sastry, M.D.

    2002-01-01

    Thermally stimulated luminescence (TSL) and electron paramagnetic resonance (EPR) studies were carried out on cerium doped and cerium-uranium co-doped K 3 Na(SO 4 ) 2 samples after γ-irradiation. Three glow peaks around 352, 415 and 475 K were observed and their spectral characteristics have shown that Ce 3+ and UO 2 2+ act as the emission centres in K 3 Na(SO 4 ) 2 :Ce and K 3 Na(SO 4 ) 2 :Ce, U, respectively. In Ce-U co-doped sample, energy transfer from cerium to uranium takes place. The commonly occurring radiation-induced centres in sulphates, viz SO 3 - and SO 4 - were observed by EPR and SO 4 - radical ion was found to take part in the TSL emission at 415 K. The hitherto unknown information, however, is the formation of SO 4 - -SO 3 - radical pair creating deep traps in these lattices, apparently assisted by the dopants. This is the first observation of such radical pair formation leading to the identification of deep traps in this lattice. The radical pair, (SO 3 - -SO 4 - ) which is stable up to 970 K, decreases the intensity of the peak at 415 K due to the depletion of SO 4 - centres

  16. Semantic Modeling for SNPs Associated with Ethnic Disparities in HapMap Samples

    Directory of Open Access Journals (Sweden)

    HyoYoung Kim

    2014-03-01

    Full Text Available Single-nucleotide polymorphisms (SNPs have been emerging out of the efforts to research human diseases and ethnic disparities. A semantic network is needed for in-depth understanding of the impacts of SNPs, because phenotypes are modulated by complex networks, including biochemical and physiological pathways. We identified ethnicity-specific SNPs by eliminating overlapped SNPs from HapMap samples, and the ethnicity-specific SNPs were mapped to the UCSC RefGene lists. Ethnicity-specific genes were identified as follows: 22 genes in the USA (CEU individuals, 25 genes in the Japanese (JPT individuals, and 332 genes in the African (YRI individuals. To analyze the biologically functional implications for ethnicity-specific SNPs, we focused on constructing a semantic network model. Entities for the network represented by "Gene," "Pathway," "Disease," "Chemical," "Drug," "ClinicalTrials," "SNP," and relationships between entity-entity were obtained through curation. Our semantic modeling for ethnicity-specific SNPs showed interesting results in the three categories, including three diseases ("AIDS-associated nephropathy," "Hypertension," and "Pelvic infection", one drug ("Methylphenidate", and five pathways ("Hemostasis," "Systemic lupus erythematosus," "Prostate cancer," "Hepatitis C virus," and "Rheumatoid arthritis". We found ethnicity-specific genes using the semantic modeling, and the majority of our findings was consistent with the previous studies - that an understanding of genetic variability explained ethnicity-specific disparities.

  17. Actividades emprendedoras y espacios alternativos de aprendizaje. Desarrollo de competencias durante un hackathon day

    Directory of Open Access Journals (Sweden)

    Carmen Ruiz Viñals

    2017-05-01

    Full Text Available El proyecto de los espacios sociales de aprendizaje en la Universidad Española se inició como parte del cambio de modelo educativo propuesto por el proceso de Bolonia. Dichos espacios los conforman todos los lugares que no son los aularios y que ayudan a completar el aprendizaje formal. En la presente investigación se reflexiona sobre estos espacios y las actividades que se desarrollan en ellos, donde los estudiantes adquieren las competencias y habilidades más demandadas por el mercado laboral, especialmente las llamadas competencias emprendedoras. Anualmente se organiza un maratón de emprendimiento llamado Barcelona Thinking Challenge en la UAO CEU. Dicha actividad es un espacio de aprendizaje donde los estudiantes adquieren competencias transversales y específicas, trabajando en equipos formados por estudiantes de diferentes cursos, universidades y profesionales. Se analizan los resultados de un estudio de encuesta que valora cuál ha sido la visión general de este maratón de emprendimiento social y qué competencias emprendedoras ha permitido desarrollar en los participantes. Los resultados permiten destacar que los partícipes valoran positivamente esta actividad emprendedora. Se muestra un alto desarrollo en las competencias relacionadas con trabajar en equipo, liderazgo y aplicación de conocimientos adquiridos en la universidad a la resolución de problemas.

  18. Effect of microbubble ligation to cells on ultrasound signal enhancement: implications for targeted imaging.

    Science.gov (United States)

    Lankford, Miles; Behm, Carolyn Z; Yeh, James; Klibanov, Alexander L; Robinson, Peter; Lindner, Jonathan R

    2006-10-01

    Molecular imaging with contrast-enhanced ultrasound (CEU) relies on the detection of microbubbles retained in regions of disease. The aim of this study was to determine whether microbubble attachment to cells influences their acoustic signal generation and stability. Biotinylated microbubbles were attached to streptavidin-coated plates to derive density versus intensity relations during low- and high-power imaging. To assess damping from microbubble attachment to solid or cell surfaces, in vitro imaging was performed for microbubbles charge-coupled to methacrylate spheres and for vascular cell adhesion molecule-1-targeted microbubbles attached to endothelial cells. Signal enhancement on plates increased according to acoustic power and microbubble site density up to 300 mm. Microbubble signal was reduced by attachment to solid spheres during high- and low-power imaging but was minimally reduced by attachment to endothelial cells and only at low power. Attachment of targeted microbubbles to rigid surfaces results in damping and a reduction of their acoustic signal, which is not seen when microbubbles are attached to cells. A reliable concentration versus intensity relationship can be expected from microbubble attachment to 2-dimensional surfaces until a very high site density is reached.

  19. Probing Fundamental Symmetries: Questioning the Very Basics of Conservation Laws

    Science.gov (United States)

    Mohanmurthy, Prajwal

    2017-09-01

    Is the Lorentz-CPT symmetry, a core component of the standard model, valid? To what extent are the CP and T symmetries broken in the strong sector? What are we doing about the existing strong-CP problem? Do neutrons oscillate (like neutral kaons) or break the (Baryon - Lepton) number conservation? In this presentation, we will go over some of the experiments probing fundamental symmetries trying to answer the above questions. I will, very briefly, introduce the CompEx & nEx experiments probing the Lorentz symmetry in the electromagnetic (EM) sector, the nEDM experiment probing CP and T symmetries in the strong sector, NStar experiment searching for neutron oscillations, MASS & BDX experiments searching for axion like particles & dark matter. We will then briefly touch upon the highlights of these experiments and focus on the path we are taking towards answering those questions while also connecting the dots [experiments] with CEU. PM would like to acknowledge support from SERI SNSF Grant 2015.0594.

  20. NDE training activities at the EPRI NDE center

    International Nuclear Information System (INIS)

    Anon.

    1987-01-01

    The need for an industry-wide qualification for NDE personnel is becoming more evident in both in-service inspection and technical skills training. ASME Section XI requirements for the qualification and certification of visual, ultrasonic, and eddy current examines is one of the major areas being supported by training at the Center. The other major thrust is in response to the Boiling Water Reactor Owners Group and its recognition of the importance of the UT operator's accurately detecting, discriminating, and sizing intergranular stress corrosion cracks (IGSCC) in piping, and inspecting weld overlay repairs of these cracked pipes. In addition, the pressurized water reactor (PWR) utilities have recognized the importance of improved eddy current data analysis of steam generator tubing. The overall intent of the Center's training is to meet the most critical utility needs with quality training that can be used by the trainee's employer as a part of its certification of that individual. To do this, the Center has organized and activated a carefully maintained documentation and records system built around the Continuing Education Unit (CEU). To address the problem of the small supply of entry-level NDE personnel available to the utilities, the Center has developed, through its Human Resource Development, academic and utility co-op programs to generate guidelines and NDE teaching materials for high schools, technical schools, and universities

  1. Editorial 2: La Nueva Teoría Estratégica y sus desarrollos en los ámbitos comunicacionales / The New Strategic Theory and its developments in the communication area

    Directory of Open Access Journals (Sweden)

    María Isabel de Salas Nestares

    2014-12-01

    Full Text Available Cuando en el año 2008, con ocasión del Congreso Internacional Brand Trends, organizado por la Universidad CEU Cardenal Herrera en Valencia, me comentó el profesor Rafael Alberto Pérez cómo había visto la aplicación de la Nueva Teoría Estratégica en las diferentes conferencias de los profesionales de comunicación comercial y corporativa allí reunidos, tomé conciencia de que efectivamente la NTE se estaba ya aplicando en el campo de la Publicidad y el Marketing, validando así el modelo o paradigma. Y por otro lado me reafirmé en mi consideración de que el proceso tecnológico de digitalización de la comunicación ha sido el magma sobre el cual la NTE ha podido avanzar en su postulado relacional. A partir de entonces seguí su consejo sobre la necesidad de realizar un cambio en “la mirada” de cómo contemplar el mundo y hacerlo rompiendo con la mirada heredada de un mundo fragmentado, con causalidad lineal y estático y pasar a una visión compleja, fluida y caótica (Pérez y Massoini, 2009: 137-142.

  2. In-silico analysis of inflammatory bowel disease (IBD GWAS loci to novel connections.

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    Md Mesbah-Uddin

    Full Text Available Genome-wide association studies (GWASs for many complex diseases, including inflammatory bowel disease (IBD, produced hundreds of disease-associated loci-the majority of which are noncoding. The number of GWAS loci is increasing very rapidly, but the process of translating single nucleotide polymorphisms (SNPs from these loci to genomic medicine is lagging. In this study, we investigated 4,734 variants from 152 IBD associated GWAS loci (IBD associated 152 lead noncoding SNPs identified from pooled GWAS results + 4,582 variants in strong linkage-disequilibrium (LD (r2 ≥0.8 for EUR population of 1K Genomes Project using four publicly available bioinformatics tools, e.g. dbPSHP, CADD, GWAVA, and RegulomeDB, to annotate and prioritize putative regulatory variants. Of the 152 lead noncoding SNPs, around 11% are under strong negative selection (GERP++ RS ≥2; and ~30% are under balancing selection (Tajima's D score >2 in CEU population (1K Genomes Project--though these regions are positively selected (GERP++ RS <0 in mammalian evolution. The analysis of 4,734 variants using three integrative annotation tools produced 929 putative functional SNPs, of which 18 SNPs (from 15 GWAS loci are in concordance with all three classifiers. These prioritized noncoding SNPs may contribute to IBD pathogenesis by dysregulating the expression of nearby genes. This study showed the usefulness of integrative annotation for prioritizing fewer functional variants from a large number of GWAS markers.

  3. The winding road to being a code monkey

    Science.gov (United States)

    Sarahan, Michael

    2017-09-01

    I am now a software engineer at a company that provides data analytics services, and helps support the open source data science community. I have been a computer nerd for a very long time, but it was my CEU experience at Texas A&M with Sherry Yennello (2003-2005) that helped me put my nerd skills to productive use. My project then was simulation of pulse shape discrimination electronics, and it was an excellent introduction to core computational concerns, such as digitization: when you see a line on the screen, that's not really how the computer sees it. I wandered in graduate school through a chemistry program into using electron microscopes. My programming interest got me into image and signal processing, which led naturally to jobs in analyzing data, and also in acquiring data. Throughout, it was always difficult just to make software work. I got pretty good at making it work. That's what I do for a living now - package software so that it is easy for other people to do great science with.

  4. PENGGUNAAN JARGON OLEH KOMUNITAS WARIA DI JEJARING SOSIAL ‘FACEBOOK’

    Directory of Open Access Journals (Sweden)

    Jusmianti Garing

    2018-03-01

    Full Text Available Jargon that used by transgender community in social networking ‘facebook’ is really interesting to understand because the jargon has a form and meaning itself in its disclosure. The research discusses of using of transgender community in social networking ‘facebook’ by using semantic parameter. The research aims to describe the jargon forms that used by transgender community in social networking ‘facebook’ and discusses the type of semantics and changes of meaning generated from the jargons. The method used of this research is qualitative descriptive by using noting and scrutinizing technique. The result shows that there are fifty-three of jargons used by transgender community in social networking ‘facebook’. Those jargons are ate, eke/eike, yey, ses, astajim, mengondek, menyabong, res/ress, le/lek/leee, mawar, ono, seljes/seljong, bo, kulo, lekes, mehong, cuccok, ce, nekk, sindang, ceu, libra, chinese, centes, peges-peges, mekong, tinta, bae, kentilas, heywanat, cacamarica, ojo, polo/polonia, mursid/mursida, bosnia, megang, kereles, kempinsky, endes, sahaja, bue, perez, dese, merongin, malides, mojang, priwi, kinyis-kinyis, cuss, eim/em, say, andbye. The jargons are formed based on the type of semantic, namely lexical meaning, grammatical meaning, denotative meaning, connotative meaning, contextual meaning, situational meaning, and thematic meaning. Furthermore, the jargons have also a relation of meaning and aspect of meaning. The relation of meaning is synonym and antonym, while, the aspect of meaning is feeling, tone, and intention. Then, there are some of jargons that undergo change the meaning, that is total meaning, broad meaning, and using diachronic parameter, such, bosnia, mursid, polo astajim, mawar, and bye. ABSTRAK Jargon yang digunakan oleh komunitas waria di jejaring sosial “‘facebook’” sangat menarik untuk dipahami karena jargon tersebut memiliki bentuk dan makna tersendiri dalam pengungkapannya. Bagi

  5. Actitud de los alumnos de quinto de medicina hacia la salud mental Medicine 5th course student's attitudes towards mental health

    Directory of Open Access Journals (Sweden)

    Inmaculada Gilaberte

    2012-12-01

    Full Text Available Introducción. El estigma social que persiste hacia la enfermedad mental puede estar presente en los estudiantes de medicina e impactar en la forma de considerar ésta y la psiquiatría como especialidad. Objetivo. Investigar la actitud hacia la enfermedad mental y la psiquiatría en una muestra de estudiantes. Sujetos y métodos. Se realizó una encuesta estructurada, voluntaria y anónima a los 27 estudiantes de quinto curso del grado de medicina de la Universidad San Pablo-CEU de Madrid. La encuesta incluía un cuestionario sobre el nivel de familiaridad hacia la enfermedad mental, el cuestionario de actitud hacia la enfermedad mental (AMI y el cuestionario adaptado de Balon sobre la actitud hacia la psiquiatría. Resultados. Un total de 22 alumnos respondió la encuesta (72,7% mujeres. Estos alumnos presentaron una alta familiaridad con la enfermedad mental (puntuación media: 7,27; máximo: 11. El 81,8% refirieron una actitud positiva hacia la enfermedad mental (escala AMI. Las puntuaciones de la escala de Balon también mostraron un nivel alto de acuerdo en los méritos globales de la psiquiatría y en la eficacia de ésta. Tampoco las respuestas respecto al rol y función del psiquiatra y a la carrera profesional revelaron una postura negativa. No hubo relación entre la familiaridad y la actitud hacia la enfermedad mental o hacia la psiquiatría. Conclusiones. En esta muestra no se evidencia la existencia de estigma hacia la enfermedad mental o hacia la psiquiatría. Posteriores estudios en muestras más amplias y evaluaciones de la enseñanza de la psiquiatría son necesarios para profundizar en este tema.Introduction. Medicine students could share that stigmatizing attitudes towards mental illness and Psychiatry as specialty. Aim. To investigate the attitudes of medical students towards the mental disease and the psychiatry. Subjects and methods. 27 medicine 5th course students from San Pablo-CEU University were interviewed with a

  6. A map of copy number variations in Chinese populations.

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    Haiyi Lou

    Full Text Available It has been shown that the human genome contains extensive copy number variations (CNVs. Investigating the medical and evolutionary impacts of CNVs requires the knowledge of locations, sizes and frequency distribution of them within and between populations. However, CNV study of Chinese minorities, which harbor the majority of genetic diversity of Chinese populations, has been underrepresented considering the same efforts in other populations. Here we constructed, to our knowledge, a first CNV map in seven Chinese populations representing the major linguistic groups in China with 1,440 CNV regions identified using Affymetrix SNP 6.0 Array. Considerable differences in distributions of CNV regions between populations and substantial population structures were observed. We showed that ∼35% of CNV regions identified in minority ethnic groups are not shared by Han Chinese population, indicating that the contribution of the minorities to genetic architecture of Chinese population could not be ignored. We further identified highly differentiated CNV regions between populations. For example, a common deletion in Dong and Zhuang (44.4% and 50%, which overlaps two keratin-associated protein genes contributing to the structure of hair fibers, was not observed in Han Chinese. Interestingly, the most differentiated CNV deletion between HapMap CEU and YRI containing CCL3L1 gene reported in previous studies was also the highest differentiated regions between Tibetan and other populations. Besides, by jointly analyzing CNVs and SNPs, we found a CNV region containing gene CTDSPL were in almost perfect linkage disequilibrium between flanking SNPs in Tibetan while not in other populations except HapMap CHD. Furthermore, we found the SNP taggability of CNVs in Chinese populations was much lower than that in European populations. Our results suggest the necessity of a full characterization of CNVs in Chinese populations, and the CNV map we constructed serves as a

  7. A Map of Copy Number Variations in Chinese Populations

    Science.gov (United States)

    Yang, Yajun; Kang, Longli; Zhang, Xin; Jin, Wenfei; Wu, Bailin; Jin, Li; Xu, Shuhua

    2011-01-01

    It has been shown that the human genome contains extensive copy number variations (CNVs). Investigating the medical and evolutionary impacts of CNVs requires the knowledge of locations, sizes and frequency distribution of them within and between populations. However, CNV study of Chinese minorities, which harbor the majority of genetic diversity of Chinese populations, has been underrepresented considering the same efforts in other populations. Here we constructed, to our knowledge, a first CNV map in seven Chinese populations representing the major linguistic groups in China with 1,440 CNV regions identified using Affymetrix SNP 6.0 Array. Considerable differences in distributions of CNV regions between populations and substantial population structures were observed. We showed that ∼35% of CNV regions identified in minority ethnic groups are not shared by Han Chinese population, indicating that the contribution of the minorities to genetic architecture of Chinese population could not be ignored. We further identified highly differentiated CNV regions between populations. For example, a common deletion in Dong and Zhuang (44.4% and 50%), which overlaps two keratin-associated protein genes contributing to the structure of hair fibers, was not observed in Han Chinese. Interestingly, the most differentiated CNV deletion between HapMap CEU and YRI containing CCL3L1 gene reported in previous studies was also the highest differentiated regions between Tibetan and other populations. Besides, by jointly analyzing CNVs and SNPs, we found a CNV region containing gene CTDSPL were in almost perfect linkage disequilibrium between flanking SNPs in Tibetan while not in other populations except HapMap CHD. Furthermore, we found the SNP taggability of CNVs in Chinese populations was much lower than that in European populations. Our results suggest the necessity of a full characterization of CNVs in Chinese populations, and the CNV map we constructed serves as a useful resource in

  8. A single-tube 27-plex SNP assay for estimating individual ancestry and admixture from three continents.

    Science.gov (United States)

    Wei, Yi-Liang; Wei, Li; Zhao, Lei; Sun, Qi-Fan; Jiang, Li; Zhang, Tao; Liu, Hai-Bo; Chen, Jian-Gang; Ye, Jian; Hu, Lan; Li, Cai-Xia

    2016-01-01

    A single-tube multiplex assay of a small set of ancestry-informative markers (AIMs) for effectively estimating individual ancestry and admixture is an ideal forensic tool to trace the population origin of an unknown DNA sample. We present a newly developed 27-plex single nucleotide polymorphism (SNP) panel with highly robust and balanced differential power to perfectly assign individuals to African, European, and East Asian ancestries. Evaluating 968 previously described intercontinental AIMs from three HapMap population genotyping datasets (Yoruban in Ibadan, Nigeria (YRI); Utah residents with Northern and Western European ancestry from the Centre de'Etude du Polymorphism Humain (CEPH) collection (CEU); and Han Chinese in Beijing, China (CHB)), the best set of markers was selected on the basis of Hardy-Weinberg equilibrium (p > 0.00001), population-specific allele frequency (two of three δ values >0.5), according to linkage disequilibrium (r (2) ancestry of the 11 populations in the HapMap project. Then, we tested the 27-plex SNP assay with 1164 individuals from 17 additional populations. The results demonstrated that the SNP panel was successful for ancestry inference of individuals with African, European, and East Asian ancestry. Furthermore, the system performed well when inferring the admixture of Eurasians (EUR/EAS) after analyzing admixed populations from Xinjiang (Central Asian) as follows: Tajik (68:27), Uyghur (49:46), Kirgiz (40:57), and Kazak (36:60). For individual analyses, we interpreted each sample with a three-ancestry component percentage and a population match probability sequence. This multiplex assay is a convenient and cost-effective tool to assist in criminal investigations, as well as to correct for the effects of population stratification for case-control studies.

  9. Sorption of Eu(III) to Kaolinite in the Presence of Humic Acids

    International Nuclear Information System (INIS)

    Shin, Hyun Sang; Harn, Yoon I; Oh, Se Jin

    2010-05-01

    The sorption of europium(Eu(III)) onto kaolinite and the influence of humic acids over a range of pH 3 ∼ 11 has been studied by batch adsorption experiment (V/m = 250:1 mL/g, CEu(III) = 1x10 -5 mol/L, C HA = 5 ∼ 50 mg/L, PCO 2 = 10 -3.5 atm). The concentrations of HA and Eu(III) in aqueous phase were measured by UV absorbance at 254 nm(e.g., UV 254 ) and ICP-MS after microwave digestion for HA removals, respectively. Results showed that the HA sorption onto kaolinite was decreased with increasing pH and their sorption isotherms fit well with the Langmuir adsorption model (except pH 3). Maximum amount(q max ) for the HA sorption at pH 4 to 11 was ranged from 4.73 to 0.47 mg/g. Europium adsorption onto the kaolinite in the absence of HA was typical, showing an increases with pH and a distinct adsorption edge at pH 3 to 5. However in the presence of HA, Eu adsorption to kaolinite was significantly affected. HA was shown to enhance Eu adsorption in the acidic pH range (pH 3 ∼ 4) due to the formation of additional binding sites for Eu coming from HA adsorbed onto kaolinite surface, but reduce Eu adsorption in the intermediate and high pH above 6 due to the formation of aqueous Eu-HA complexes. The results on the ternary interaction of kaolinite-Eu-HA are compared with those on the binary system of kaolinite-HA and kaolinite-Eu and adsorption mechanism with pH was discussed

  10. ANÁLISE DO PERFIL CLÍNICO DOS PACIENTES ATENDIDOS NA ESPECIALIDADE DE FISIOTERAPIA DERMATOFUNCIONAL NA CLÍNICA ESCOLA DE UMA INSTITUIÇÃO DE ENSINO SUPERIOR

    Directory of Open Access Journals (Sweden)

    Jhenifer Santiago de Oliveira

    2016-06-01

    Full Text Available Objective: establish the clinical profile of patients seen at a clinic physical therapy school in dermatofuncional physical therapy specialty. Methods: descriptive study, carried out by patient charts analysis met from February 2013 to November 2014, at the Clinic UniFisio School (CEU in the city of Anapolis, Goias. Results: 48 records were analyzed, these 72 92 %% female and 25% male. 16.67% have fibroedemagelóide, leprosy 20.83%, 8.33% had burns, 10.42% related to wrinkles, 10.42% had stretch marks, cancer 8.33%, 14.58% and 10 lymphedema, 42% other isolated cases. The main complaints were described pain with 24%, then 22% the loss of mobility after scar with 15%, cellulite and stretch marks with 11%, 9% decrease in amplitude of the upper limbs and 8% of the marks of expression. In analysis of physical activity frequency 58.33% of the charts analyzed patients were sedentary, 16.67% regularly practice some form of physical activity and 25% did not declare. Regarding the diagnosis has fibroedemagelóide 16.67%, 20.83% leprosy, 8.33% had burns, 10.42% related to wrinkles, 10.42% had stretch marks, cancer 8.33%, 14.58% lymphedema and even had cases that were isolated characterized as others for a total of 10.42%. conclusions: The profile goers in most patients was between 30-40 years, with most of diagnosis of leprosy and complaining of pain.

  11. Cuidado que el medicamento no se te salga por la nariz

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    Feijoo Calles D

    2017-12-01

    Full Text Available La enfermedad pulmonar obstructiva crónica (EPOC es una patología caracterizada por una reducción persistente del flujo de aire. Esta enfermedad pulmonar es progresiva y potencialmente mortal. Suele causar disnea y las exacerbaciones y comorbilidades asociadas hacen que el pronóstico sea peor. Se presenta un caso de seguimiento farmacoterapéutico realizado siguiendo la metodología del Foro de Atención Farmacéutica en Farmacia Comunitaria en el Aula de Práctica Farmacéutica “Paco Martínez” de la Universidad CEU Cardenal Herrera a un paciente real con EPOC y polimedicado, utilizando como método el “aprendizaje basado en problemas”. Como apoyo para la evaluación de la situación se realiza un análisis de las diferentes teorías publicadas sobre la ineficacia del tratamiento. Además, se expone el nuevo enfoque que se le quiere dar al tratamiento de los pacientes con EPOC, valorando la heterogeneidad de la enfermedad y la variabilidad clínica de cada paciente mediante la revisión bibliográfica en bases de datos científicas Pubmed/Medline y Google Académico, junto con la búsqueda de información en las diferentes páginas web de organismos oficiales, Organización Mundial de la Salud y Agencia Española de Medicamentos y Productos Sanitarios.

  12. Chromosome-encoded narrow-spectrum Ambler class A beta-lactamase GIL-1 from Citrobacter gillenii.

    Science.gov (United States)

    Naas, Thierry; Aubert, Daniel; Ozcan, Ayla; Nordmann, Patrice

    2007-04-01

    A novel beta-lactamase gene was cloned from the whole-cell DNA of an enterobacterial Citrobacter gillenii reference strain that displayed a weak narrow-spectrum beta-lactam-resistant phenotype and was expressed in Escherichia coli. It encoded a clavulanic acid-inhibited Ambler class A beta-lactamase, GIL-1, with a pI value of 7.5 and a molecular mass of ca. 29 kDa. GIL-1 had the highest percent amino acid sequence identity with TEM-1 and SHV-1, 77%, and 67%, respectively, and only 46%, 31%, and 32% amino acid sequence identity with CKO-1 (C. koseri), CdiA1 (C. diversus), and SED-1 (C. sedlaki), respectively. The substrate profile of the purified GIL-1 was similar to that of beta-lactamases TEM-1 and SHV-1. The blaGIL-1 gene was chromosomally located, as revealed by I-CeuI experiments, and was constitutively expressed at a low level in C. gillenii. No gene homologous to the regulatory ampR genes of chromosomal class C beta-lactamases was found upstream of the blaGIL-1 gene, which fits the noninducibility of beta-lactamase expression in C. gillenii. Rapid amplification of DNA 5' ends analysis of the promoter region revealed putative promoter sequences that diverge from what has been identified as the consensus sequence in E. coli. The blaGIL-1 gene was part of a 5.5-kb DNA fragment bracketed by a 9-bp duplication and inserted between the d-lactate dehydrogenase gene and the ydbH genes; this DNA fragment was absent in other Citrobacter species. This work further illustrates the heterogeneity of beta-lactamases in Citrobacter spp., which may indicate that the variability of Citrobacter species is greater than expected.

  13. GLIDERS - A web-based search engine for genome-wide linkage disequilibrium between HapMap SNPs

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    Broxholme John

    2009-10-01

    Full Text Available Abstract Background A number of tools for the examination of linkage disequilibrium (LD patterns between nearby alleles exist, but none are available for quickly and easily investigating LD at longer ranges (>500 kb. We have developed a web-based query tool (GLIDERS: Genome-wide LInkage DisEquilibrium Repository and Search engine that enables the retrieval of pairwise associations with r2 ≥ 0.3 across the human genome for any SNP genotyped within HapMap phase 2 and 3, regardless of distance between the markers. Description GLIDERS is an easy to use web tool that only requires the user to enter rs numbers of SNPs they want to retrieve genome-wide LD for (both nearby and long-range. The intuitive web interface handles both manual entry of SNP IDs as well as allowing users to upload files of SNP IDs. The user can limit the resulting inter SNP associations with easy to use menu options. These include MAF limit (5-45%, distance limits between SNPs (minimum and maximum, r2 (0.3 to 1, HapMap population sample (CEU, YRI and JPT+CHB combined and HapMap build/release. All resulting genome-wide inter-SNP associations are displayed on a single output page, which has a link to a downloadable tab delimited text file. Conclusion GLIDERS is a quick and easy way to retrieve genome-wide inter-SNP associations and to explore LD patterns for any number of SNPs of interest. GLIDERS can be useful in identifying SNPs with long-range LD. This can highlight mis-mapping or other potential association signal localisation problems.

  14. Genotype Imputation for Latinos Using the HapMap and 1000 Genomes Project Reference Panels

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    Xiaoyi eGao

    2012-06-01

    Full Text Available Genotype imputation is a vital tool in genome-wide association studies (GWAS and meta-analyses of multiple GWAS results. Imputation enables researchers to increase genomic coverage and to pool data generated using different genotyping platforms. HapMap samples are often employed as the reference panel. More recently, the 1000 Genomes Project resource is becoming the primary source for reference panels. Multiple GWAS and meta-analyses are targeting Latinos, the most populous and fastest growing minority group in the US. However, genotype imputation resources for Latinos are rather limited compared to individuals of European ancestry at present, largely because of the lack of good reference data. One choice of reference panel for Latinos is one derived from the population of Mexican individuals in Los Angeles contained in the HapMap Phase 3 project and the 1000 Genomes Project. However, a detailed evaluation of the quality of the imputed genotypes derived from the public reference panels has not yet been reported. Using simulation studies, the Illumina OmniExpress GWAS data from the Los Angles Latino Eye Study and the MACH software package, we evaluated the accuracy of genotype imputation in Latinos. Our results show that the 1000 Genomes Project AMR+CEU+YRI reference panel provides the highest imputation accuracy for Latinos, and that also including Asian samples in the panel can reduce imputation accuracy. We also provide the imputation accuracy for each autosomal chromosome using the 1000 Genomes Project panel for Latinos. Our results serve as a guide to future imputation-based analysis in Latinos.

  15. An unusual haplotype structure on human chromosome 8p23 derived from the inversion polymorphism.

    Science.gov (United States)

    Deng, Libin; Zhang, Yuezheng; Kang, Jian; Liu, Tao; Zhao, Hongbin; Gao, Yang; Li, Chaohua; Pan, Hao; Tang, Xiaoli; Wang, Dunmei; Niu, Tianhua; Yang, Huanming; Zeng, Changqing

    2008-10-01

    Chromosomal inversion is an important type of genomic variations involved in both evolution and disease pathogenesis. Here, we describe the refined genetic structure of a 3.8-Mb inversion polymorphism at chromosome 8p23. Using HapMap data of 1,073 SNPs generated from 209 unrelated samples from CEPH-Utah residents with ancestry from northern and western Europe (CEU); Yoruba in Ibadan, Nigeria (YRI); and Asian (ASN) samples, which were comprised of Han Chinese from Beijing, China (CHB) and Japanese from Tokyo, Japan (JPT)-we successfully deduced the inversion orientations of all their 418 haplotypes. In particular, distinct haplotype subgroups were identified based on principal component analysis (PCA). Such genetic substructures were consistent with clustering patterns based on neighbor-joining tree reconstruction, which revealed a total of four haplotype clades across all samples. Metaphase fluorescence in situ hybridization (FISH) in a subset of 10 HapMap samples verified their inversion orientations predicted by PCA or phylogenetic tree reconstruction. Positioning of the outgroup haplotype within one of YRI clades suggested that Human NCBI Build 36-inverted order is most likely the ancestral orientation. Furthermore, the population differentiation test and the relative extended haplotype homozygosity (REHH) analysis in this region discovered multiple selection signals, also in a population-specific manner. A positive selection signal was detected at XKR6 in the ASN population. These results revealed the correlation of inversion polymorphisms to population-specific genetic structures, and various selection patterns as possible mechanisms for the maintenance of a large chromosomal rearrangement at 8p23 region during evolution. In addition, our study also showed that haplotype-based clustering methods, such as PCA, can be applied in scanning for cryptic inversion polymorphisms at a genome-wide scale.

  16. New Software for the Fast Estimation of Population Recombination Rates (FastEPRR in the Genomic Era

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    Feng Gao

    2016-06-01

    Full Text Available Genetic recombination is a very important evolutionary mechanism that mixes parental haplotypes and produces new raw material for organismal evolution. As a result, information on recombination rates is critical for biological research. In this paper, we introduce a new extremely fast open-source software package (FastEPRR that uses machine learning to estimate recombination rate ρ (=4Ner from intraspecific DNA polymorphism data. When ρ>10 and the number of sampled diploid individuals is large enough (≥50, the variance of ρFastEPRR remains slightly smaller than that of ρLDhat. The new estimate ρcomb (calculated by averaging ρFastEPRR and ρLDhat has the smallest variance of all cases. When estimating ρFastEPRR, the finite-site model was employed to analyze cases with a high rate of recurrent mutations, and an additional method is proposed to consider the effect of variable recombination rates within windows. Simulations encompassing a wide range of parameters demonstrate that different evolutionary factors, such as demography and selection, may not increase the false positive rate of recombination hotspots. Overall, accuracy of FastEPRR is similar to the well-known method, LDhat, but requires far less computation time. Genetic maps for each human population (YRI, CEU, and CHB extracted from the 1000 Genomes OMNI data set were obtained in less than 3 d using just a single CPU core. The Pearson Pairwise correlation coefficient between the ρFastEPRR and ρLDhat maps is very high, ranging between 0.929 and 0.987 at a 5-Mb scale. Considering that sample sizes for these kinds of data are increasing dramatically with advances in next-generation sequencing technologies, FastEPRR (freely available at http://www.picb.ac.cn/evolgen/ is expected to become a widely used tool for establishing genetic maps and studying recombination hotspots in the population genomic era.

  17. Influence of leukotriene gene polymorphisms on chronic rhinosinusitis

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    Duval Melanie

    2008-03-01

    Full Text Available Abstract Background Chronic rhinosinusitis (CRS is increasingly viewed as an inflammatory condition of the sinonasal mucosa interacting with bacteria and/or fungi. However, factors conferring susceptibility to disease remain unknown. Advances in genomics offer powerful tools to explore this disorder. The goal of this study was to evaluate the effect of single nucleotide polymorphisms (SNP on CRS in a panel of genes related to cysteinyl leukotriene metabolism. Methods Severe cases of CRS and postal code match controls were recruited prospectively. A total of 206 cases and 200 controls were available for the present study. Using a candidate gene approach, five genes related to cysteinyl leukotriene metabolism were assessed. For each gene, we selected the maximally informative set of common SNPs (tagSNPs using the European-derived (CEU HapMap dataset. These SNPs are in arachidonate 5-lipoxygenase (ALOX5, arachidonate 5-lipoxygenase-activating protein (ALOX5AP, leukotriene C4 synthase (LTC4S, cysteinyl leukotriene receptor 1 (CYSLTR1 and cysteinyl leukotriene receptor 2 (CYSLTR2 genes. Results A total of 59 SNPs were genotyped to capture the common genetic variations within these genes. Three SNPs located within the ALOX5, CYSLTR1 and ALOX5AP genes reached the nominal p-value threshold (p Conclusion While these initial results do not support that polymorphsims in genes assessed involved in the leukotriene pathways are contributing to the pathogenesis of CRS, this initial study was not powered to detect polymorphisms with relative risk of 2.0 or less, where we could expect many gene effects for complex diseases to occur. Thus, despite this lack of significant association noted in this study, we believe that validation with external populations and the use of better-powered studies in the future may allow more conclusive findings.

  18. Whole genome PCR scanning reveals the syntenic genome structure of toxigenic Vibrio cholerae strains in the O1/O139 population.

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    Bo Pang

    Full Text Available Vibrio cholerae is commonly found in estuarine water systems. Toxigenic O1 and O139 V. cholerae strains have caused cholera epidemics and pandemics, whereas the nontoxigenic strains within these serogroups only occasionally lead to disease. To understand the differences in the genome and clonality between the toxigenic and nontoxigenic strains of V. cholerae serogroups O1 and O139, we employed a whole genome PCR scanning (WGPScanning method, an rrn operon-mediated fragment rearrangement analysis and comparative genomic hybridization (CGH to analyze the genome structure of different strains. WGPScanning in conjunction with CGH revealed that the genomic contents of the toxigenic strains were conservative, except for a few indels located mainly in mobile elements. Minor nucleotide variation in orthologous genes appeared to be the major difference between the toxigenic strains. rrn operon-mediated rearrangements were infrequent in El Tor toxigenic strains tested using I-CeuI digested pulsed-field gel electrophoresis (PFGE analysis and PCR analysis based on flanking sequence of rrn operons. Using these methods, we found that the genomic structures of toxigenic El Tor and O139 strains were syntenic. The nontoxigenic strains exhibited more extensive sequence variations, but toxin coregulated pilus positive (TCP+ strains had a similar structure. TCP+ nontoxigenic strains could be subdivided into multiple lineages according to the TCP type, suggesting the existence of complex intermediates in the evolution of toxigenic strains. The data indicate that toxigenic O1 El Tor and O139 strains were derived from a single lineage of intermediates from complex clones in the environment. The nontoxigenic strains with non-El Tor type TCP may yet evolve into new epidemic clones after attaining toxigenic attributes.

  19. Actitud de los alumnos de quinto de medicina hacia la salud mental

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    Inmaculada Gilaberte

    Full Text Available Introducción. El estigma social que persiste hacia la enfermedad mental puede estar presente en los estudiantes de medicina e impactar en la forma de considerar ésta y la psiquiatría como especialidad. Objetivo. Investigar la actitud hacia la enfermedad mental y la psiquiatría en una muestra de estudiantes. Sujetos y métodos. Se realizó una encuesta estructurada, voluntaria y anónima a los 27 estudiantes de quinto curso del grado de medicina de la Universidad San Pablo-CEU de Madrid. La encuesta incluía un cuestionario sobre el nivel de familiaridad hacia la enfermedad mental, el cuestionario de actitud hacia la enfermedad mental (AMI y el cuestionario adaptado de Balon sobre la actitud hacia la psiquiatría. Resultados. Un total de 22 alumnos respondió la encuesta (72,7% mujeres. Estos alumnos presentaron una alta familiaridad con la enfermedad mental (puntuación media: 7,27; máximo: 11. El 81,8% refirieron una actitud positiva hacia la enfermedad mental (escala AMI. Las puntuaciones de la escala de Balon también mostraron un nivel alto de acuerdo en los méritos globales de la psiquiatría y en la eficacia de ésta. Tampoco las respuestas respecto al rol y función del psiquiatra y a la carrera profesional revelaron una postura negativa. No hubo relación entre la familiaridad y la actitud hacia la enfermedad mental o hacia la psiquiatría. Conclusiones. En esta muestra no se evidencia la existencia de estigma hacia la enfermedad mental o hacia la psiquiatría. Posteriores estudios en muestras más amplias y evaluaciones de la enseñanza de la psiquiatría son necesarios para profundizar en este tema.

  20. Patterns of population differentiation of candidate genes for cardiovascular disease

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    Ding Keyue

    2007-07-01

    Full Text Available Abstract Background The basis for ethnic differences in cardiovascular disease (CVD susceptibility is not fully understood. We investigated patterns of population differentiation (FST of a set of genes in etiologic pathways of CVD among 3 ethnic groups: Yoruba in Nigeria (YRI, Utah residents with European ancestry (CEU, and Han Chinese (CHB + Japanese (JPT. We identified 37 pathways implicated in CVD based on the PANTHER classification and 416 genes in these pathways were further studied; these genes belonged to 6 biological processes (apoptosis, blood circulation and gas exchange, blood clotting, homeostasis, immune response, and lipoprotein metabolism. Genotype data were obtained from the HapMap database. Results We calculated FST for 15,559 common SNPs (minor allele frequency ≥ 0.10 in at least one population in genes that co-segregated among the populations, as well as an average-weighted FST for each gene. SNPs were classified as putatively functional (non-synonymous and untranslated regions or non-functional (intronic and synonymous sites. Mean FST values for common putatively functional variants were significantly higher than FST values for nonfunctional variants. A significant variation in FST was also seen based on biological processes; the processes of 'apoptosis' and 'lipoprotein metabolism' showed an excess of genes with high FST. Thus, putative functional SNPs in genes in etiologic pathways for CVD show greater population differentiation than non-functional SNPs and a significant variance of FST values was noted among pairwise population comparisons for different biological processes. Conclusion These results suggest a possible basis for varying susceptibility to CVD among ethnic groups.

  1. Genotypic and allelic variability in CYP19A1 among populations of African and European ancestry.

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    Athena Starlard-Davenport

    Full Text Available CYP19A1 facilitates the bioconversion of estrogens from androgens. CYP19A1 intron single nucleotide polymorphisms (SNPs may alter mRNA splicing, resulting in altered CYP19A1 activity, and potentially influencing disease susceptibility. Genetic studies of CYP19A1 SNPs have been well documented in populations of European ancestry; however, studies in populations of African ancestry are limited. In the present study, ten 'candidate' intronic SNPs in CYP19A1 from 125 African Americans (AA and 277 European Americans (EA were genotyped and their frequencies compared. Allele frequencies were also compared with HapMap and ASW 1000 Genomes populations. We observed significant differences in the minor allele frequencies between AA and EA in six of the ten SNPs including rs10459592 (p<0.0001, rs12908960 (p<0.0001, rs1902584 (p = 0.016, rs2470144 (p<0.0001, rs1961177 (p<0.0001, and rs6493497 (p = 0.003. While there were no significant differences in allele frequencies between EA and CEU in the HapMap population, a 1.2- to 19-fold difference in allele frequency for rs10459592 (p = 0.004, rs12908960 (p = 0.0006, rs1902584 (p<0.0001, rs2470144 (p = 0.0006, rs1961177 (p<0.0001, and rs6493497 (p = 0.0092 was observed between AA and the Yoruba (YRI population. Linkage disequilibrium (LD blocks and haplotype clusters that is unique to the EA population but not AA was also observed. In summary, we demonstrate that differences in the allele frequencies of CYP19A1 intron SNPs are not consistent between populations of African and European ancestry. Thus, investigations into whether CYP19A1 intron SNPs contribute to variations in cancer incidence, outcomes and pharmacological response seen in populations of different ancestry may prove beneficial.

  2. Worldwide distribution of the MYH9 kidney disease susceptibility alleles and haplotypes: evidence of historical selection in Africa.

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    Taras K Oleksyk

    2010-07-01

    Full Text Available MYH9 was recently identified as renal susceptibility gene (OR 3-8, p or = 60% than in European Americans (< 4%, revealing a genetic basis for a major health disparity. The population distributions of MYH9 risk alleles and the E-1 risk haplotype and the demographic and selective forces acting on the MYH9 region are not well explored. We reconstructed MYH9 haplotypes from 4 tagging single nucleotide polymorphisms (SNPs spanning introns 12-23 using available data from HapMap Phase II, and by genotyping 938 DNAs from the Human Genome Diversity Panel (HGDP. The E-1 risk haplotype followed a cline, being most frequent within sub-Saharan African populations (range 50-80%, less frequent in populations from the Middle East (9-27% and Europe (0-9%, and rare or absent in Asia, the Americas, and Oceania. The fixation indexes (F(ST for pairwise comparisons between the risk haplotypes for continental populations were calculated for MYH9 haplotypes; F(ST ranged from 0.27-0.40 for Africa compared to other continental populations, possibly due to selection. Uniquely in Africa, the Yoruba population showed high frequency extended haplotype length around the core risk allele (C compared to the alternative allele (T at the same locus (rs4821481, iHs = 2.67, as well as high population differentiation (F(ST(CEU vs. YRI = 0.51 in HapMap Phase II data, also observable only in the Yoruba population from HGDP (F(ST = 0.49, pointing to an instance of recent selection in the genomic region. The population-specific divergence in MYH9 risk allele frequencies among the world's populations may prove important in risk assessment and public health policies to mitigate the burden of kidney disease in vulnerable populations.

  3. Comparison of Genetic Variants in Cancer-Related Genes between Chinese Hui and Han Populations.

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    Chaoyong Tian

    Full Text Available The Chinese Hui population, as the second largest minority ethnic group in China, may have a different genetic background from Han people because of its unique demographic history. In this study, we aimed to identify genetic differences between Han and Hui Chinese from the Ningxia region of China by comparing eighteen single nucleotide polymorphisms in cancer-related genes.DNA samples were collected from 99 Hui and 145 Han people from the Ningxia Hui Autonomous Region in China, and SNPs were detected using an improved multiplex ligase detection reaction method. Genotyping data from six 1000 Genomes Project population samples (99 Utah residents with northern and western European ancestry (CEU, 107 Toscani in Italy (TSI, 108 Yoruba in Ibadan (YRI, 61 of African ancestry in the southwestern US (ASW, 103 Han Chinese in Beijing (CHB, and 104 Japanese in Tokyo (JPT were also included in this study. Differences in the distribution of alleles among the populations were assessed using χ2 tests, and FST was used to measure the degree of population differentiation.We found that the genetic diversity of many SNPs in cancer-related genes in the Hui Chinese in Ningxia was different from that in the Han Chinese in Ningxia. For example, the allele frequencies of four SNPs (rs13361707, rs2274223, rs465498, and rs753955 showed different genetic distributions (p0.000 between the Hui and Han populations.These results suggest that some SNPs associated with cancer-related genes vary among different Chinese ethnic groups. We suggest that population differences should be carefully considered in evaluating cancer risk and prognosis as well as the efficacy of cancer therapy.

  4. Effective selection of informative SNPs and classification on the HapMap genotype data

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    Wang Lipo

    2007-12-01

    Full Text Available Abstract Background Since the single nucleotide polymorphisms (SNPs are genetic variations which determine the difference between any two unrelated individuals, the SNPs can be used to identify the correct source population of an individual. For efficient population identification with the HapMap genotype data, as few informative SNPs as possible are required from the original 4 million SNPs. Recently, Park et al. (2006 adopted the nearest shrunken centroid method to classify the three populations, i.e., Utah residents with ancestry from Northern and Western Europe (CEU, Yoruba in Ibadan, Nigeria in West Africa (YRI, and Han Chinese in Beijing together with Japanese in Tokyo (CHB+JPT, from which 100,736 SNPs were obtained and the top 82 SNPs could completely classify the three populations. Results In this paper, we propose to first rank each feature (SNP using a ranking measure, i.e., a modified t-test or F-statistics. Then from the ranking list, we form different feature subsets by sequentially choosing different numbers of features (e.g., 1, 2, 3, ..., 100. with top ranking values, train and test them by a classifier, e.g., the support vector machine (SVM, thereby finding one subset which has the highest classification accuracy. Compared to the classification method of Park et al., we obtain a better result, i.e., good classification of the 3 populations using on average 64 SNPs. Conclusion Experimental results show that the both of the modified t-test and F-statistics method are very effective in ranking SNPs about their classification capabilities. Combined with the SVM classifier, a desirable feature subset (with the minimum size and most informativeness can be quickly found in the greedy manner after ranking all SNPs. Our method is able to identify a very small number of important SNPs that can determine the populations of individuals.

  5. Signatures of natural selection at the FTO (fat mass and obesity associated locus in human populations.

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    Xuanshi Liu

    Full Text Available Polymorphisms in the first intron of FTO have been robustly replicated for associations with obesity. In the Sorbs, a Slavic population resident in Germany, the strongest effect on body mass index (BMI was found for a variant in the third intron of FTO (rs17818902. Since this may indicate population specific effects of FTO variants, we initiated studies testing FTO for signatures of selection in vertebrate species and human populations.First, we analyzed the coding region of 35 vertebrate FTO orthologs with Phylogenetic Analysis by Maximum Likelihood (PAML, ω = dN/dS to screen for signatures of selection among species. Second, we investigated human population (Europeans/CEU, Yoruba/YRI, Chinese/CHB, Japanese/JPT, Sorbs SNP data for footprints of selection using DnaSP version 4.5 and the Haplotter/PhaseII. Finally, using ConSite we compared transcription factor (TF binding sites at sequences harbouring FTO SNPs in intron three.PAML analyses revealed strong conservation in coding region of FTO (ω<1. Sliding-window results from population genetic analyses provided highly significant (p<0.001 signatures for balancing selection specifically in the third intron (e.g. Tajima's D in Sorbs = 2.77. We observed several alterations in TF binding sites, e.g. TCF3 binding site introduced by the rs17818902 minor allele.Population genetic analysis revealed signatures of balancing selection at the FTO locus with a prominent signal in intron three, a genomic region with strong association with BMI in the Sorbs. Our data support the hypothesis that genes associated with obesity may have been under evolutionary selective pressure.

  6. Variation in regulator of G-protein signaling 17 gene (RGS17 is associated with multiple substance dependence diagnoses

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    Zhang Huiping

    2012-05-01

    Full Text Available Abstract Background RGS17 and RGS20 encode two members of the regulator of G-protein signaling RGS-Rz subfamily. Variation in these genes may alter their transcription and thereby influence the function of G protein-coupled receptors, including opioid receptors, and modify risk for substance dependence. Methods The association of 13 RGS17 and eight RGS20 tag single nucleotide polymorphisms (SNPs was examined with four substance dependence diagnoses (alcohol (AD, cocaine (CD, opioid (OD or marijuana (MjD] in 1,905 African Americans (AAs: 1,562 cases and 343 controls and 1,332 European Americans (EAs: 981 cases and 351 controls. Analyses were performed using both χ2 tests and logistic regression analyses that covaried sex, age, and ancestry proportion. Correlation of genotypes and mRNA expression levels was assessed by linear regression analyses. Results Seven RGS17 SNPs showed a significant association with at least one of the four dependence traits after a permutation-based correction for multiple testing (0.003≤Pempirical≤0.037. The G allele of SNP rs596359, in the RGS17 promoter region, was associated with AD, CD, OD, or MjD in both populations (0.005≤Pempirical≤0.019. This allele was also associated with significantly lower mRNA expression levels of RGS17 in YRI subjects (P = 0.002 and non-significantly lower mRNA expression levels of RGS17 in CEU subjects (P = 0.185. No RGS20 SNPs were associated with any of the four dependence traits in either population. Conclusions This study demonstrated that variation in RGS17 was associated with risk for substance dependence diagnoses in both AA and EA populations.

  7. Distribution and behavior of major and trace elements in Tokyo Bay, Mutsu Bay and Funka Bay marine sediments

    International Nuclear Information System (INIS)

    Honda, Teruyuki; Kimura, Ken-ichiro

    2003-01-01

    Fourteen major and trace elements in marine sediment core samples collected from the coasts along eastern Japan, i.e. Tokyo Bay (II) (the recess), Tokyo Bay (IV) (the mouth), Mutsu Bay and Funka Bay and the Northwest Pacific basin as a comparative subject were determined by the instrumental neutron activation analysis (INAA). The sedimentation rates and sedimentary ages were calculated for the coastal sediment cores by the 210 Pb method. The results obtained in this study are summarized as follows: (1) Lanthanoid abundance patterns suggested that the major origin of the sediments was terrigenous material. La*/Lu* and Ce*/La* ratios revealed that the sediments from Tokyo Bay (II) and Mutsu Bay more directly reflected the contribution from river than those of other regions. In addition, the Th/Sc ratio indicated that the coastal sediments mainly originated in the materials from the volcanic island-arcs, Japanese islands, whereas those from the Northwest Pacific mainly from the continent. (2) The correlation between the Ce/U and Th/U ratios with high correlation coefficients of 0.920 to 0.991 indicated that all the sediments from Tokyo Bay (II) and Funka Bay were in reducing conditions while at least the upper sediments from Tokyo Bay (IV) and Mutsu Bay were in oxidizing conditions. (3) It became quite obvious that the sedimentation mechanism and the sedimentation environment at Tokyo Bay (II) was different from those at Tokyo Bay (IV), since the sedimentation rate at Tokyo Bay (II) was approximately twice as large as that at Tokyo Bay (IV). The sedimentary age of the 5th layer (8∼10 cm in depth) from Funka Bay was calculated at approximately 1940∼50, which agreed with the time, 1943∼45 when Showa-shinzan was formed by the eruption of the Usu volcano. (author)

  8. Occupational exposure to EM radiation in the range from 800 to 2500 MHz : What are we really talking about?

    International Nuclear Information System (INIS)

    Prlic, I.; Suric Mihic, M.

    2008-01-01

    Full text: Precautionary principle is explicitly laid down in the EU treaty and is one of the starting points of its environmental policy. European Commission made a statement in February 2000 regarding the use of precautionary principle indicating that principle should be applied where there is a reasonable suspicion of the existence of a health or environmental risk. Furthermore, the Commission indicates that measures based on the precautionary principle should not be aimed at completely precluding any risk; it assumes that such an effort is unrealistic. In the Council Recommendation of 12 July 1999 on the limitation of exposure of the general public to electromagnetic fields (0 Hz to 300 GHz) (CEU) the following comment is made in Appendix I: 'However, since there is a safety factor of about 50 between the threshold values for acute effects and the basis restrictions, this recommendation implicitly covers possible long-term effects in the whole frequency range.' Both, the comment and the Council Recommendation are addressing the human health. This concern over human health was raised up because modern society is highly sensitive to extended growth of mobile and wireless telecommunication technologies regardless of their obvious economic and societal benefits. This growth was followed with the changes in working environment and with gestation of new types of working places. Occupational medicine is challenged with the new working stressor which has to be scientifically acceptable introduced in daily life. Natural nonionizing background radiation is covering the globe with much more complex space matrix than natural ionizing background radiation. There is a decision to be made weather the occupational exposure to RF is to be regarded as potentially hazardous or is simply to be added to a person total exposure to such fields. We are proposing the reasonable protocol for the how to decide and judge the occupational exposure to RF fields originating from

  9. Large scale association analysis identifies three susceptibility loci for coronary artery disease.

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    Stephanie Saade

    Full Text Available Genome wide association studies (GWAS and their replications that have associated DNA variants with myocardial infarction (MI and/or coronary artery disease (CAD are predominantly based on populations of European or Eastern Asian descent. Replication of the most significantly associated polymorphisms in multiple populations with distinctive genetic backgrounds and lifestyles is crucial to the understanding of the pathophysiology of a multifactorial disease like CAD. We have used our Lebanese cohort to perform a replication study of nine previously identified CAD/MI susceptibility loci (LTA, CDKN2A-CDKN2B, CELSR2-PSRC1-SORT1, CXCL12, MTHFD1L, WDR12, PCSK9, SH2B3, and SLC22A3, and 88 genes in related phenotypes. The study was conducted on 2,002 patients with detailed demographic, clinical characteristics, and cardiac catheterization results. One marker, rs6922269, in MTHFD1L was significantly protective against MI (OR=0.68, p=0.0035, while the variant rs4977574 in CDKN2A-CDKN2B was significantly associated with MI (OR=1.33, p=0.0086. Associations were detected after adjustment for family history of CAD, gender, hypertension, hyperlipidemia, diabetes, and smoking. The parallel study of 88 previously published genes in related phenotypes encompassed 20,225 markers, three quarters of which with imputed genotypes The study was based on our genome-wide genotype data set, with imputation across the whole genome to HapMap II release 22 using HapMap CEU population as a reference. Analysis was conducted on both the genotyped and imputed variants in the 88 regions covering selected genes. This approach replicated HNRNPA3P1-CXCL12 association with CAD and identified new significant associations of CDKAL1, ST6GAL1, and PTPRD with CAD. Our study provides evidence for the importance of the multifactorial aspect of CAD/MI and describes genes predisposing to their etiology.

  10. The use of genome-wide eQTL associations in lymphoblastoid cell lines to identify novel genetic pathways involved in complex traits.

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    Josine L Min

    Full Text Available The integrated analysis of genotypic and expression data for association with complex traits could identify novel genetic pathways involved in complex traits. We profiled 19,573 expression probes in Epstein-Barr virus-transformed lymphoblastoid cell lines (LCLs from 299 twins and correlated these with 44 quantitative traits (QTs. For 939 expressed probes correlating with more than one QT, we investigated the presence of eQTL associations in three datasets of 57 CEU HapMap founders and 86 unrelated twins. Genome-wide association analysis of these probes with 2.2 m SNPs revealed 131 potential eQTLs (1,989 eQTL SNPs overlapping between the HapMap datasets, five of which were in cis (58 eQTL SNPs. We then tested 535 SNPs tagging the eQTL SNPs, for association with the relevant QT in 2,905 twins. We identified nine potential SNP-QT associations (P<0.01 but none significantly replicated in five large consortia of 1,097-16,129 subjects. We also failed to replicate previous reported eQTL associations with body mass index, plasma low-density lipoprotein cholesterol, high-density lipoprotein cholesterol and triglycerides levels derived from lymphocytes, adipose and liver tissue. Our results and additional power calculations suggest that proponents may have been overoptimistic in the power of LCLs in eQTL approaches to elucidate regulatory genetic effects on complex traits using the small datasets generated to date. Nevertheless, larger tissue-specific expression data sets relevant to specific traits are becoming available, and should enable the adoption of similar integrated analyses in the near future.

  11. Profile of guanfacine extended release and its potential in the treatment of attention-deficit hyperactivity disorder

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    Martinez-Raga J

    2015-05-01

    Full Text Available Jose Martinez-Raga,1,2 Carlos Knecht,3 Raquel de Alvaro4 1Teaching Unit of Psychiatry and Psychological Medicine, University Hospital Doctor Peset, University of Valencia, 2CEU Cardenal Herrera University, 3Área de Salud Mental, Hospital Padre Jofré, Valencia, 4Hospital General, Consorcio Hospitalario Provincial, Castellon, Spain Abstract: The α2-adrenergic receptor agonist guanfacine, in its extended-release formulation (GXR, is the most recent nonstimulant medication approved in several countries for the treatment of attention-deficit hyperactivity disorder (ADHD as monotherapy and as adjunctive pharmacotherapy to stimulants in children and adolescents. The present paper aims to review comprehensively and critically the pharmacodynamic and pharmacokinetic characteristics and the published evidence on the efficacy and safety profile of GXR in the treatment of ADHD. A comprehensive search of relevant databases (PubMed, Embase, and PsycInfo was conducted to identify studies published in peer-reviewed journals until January 15, 2015. Though the precise mechanism of action of guanfacine in the treatment of ADHD is not fully understood, it is thought to act directly by enhancing noradrenaline functioning via α2A-adrenoceptors in the prefrontal cortex. Weight-adjusted doses should be used, with a dosing regime on a milligram per kilogram basis, starting at doses in the range 0.05–0.08 mg/kg/day, up to 0.12 mg/kg/day. As evidenced in short-term randomized controlled trials and in long-term open-label extension studies, GXR has been shown to be effective as monotherapy in the treatment of ADHD. Furthermore, GXR has also been found to be effective as adjunctive therapy to stimulant medications in patients with suboptimal responses to stimulants. Many of the adverse reactions associated with GXR, particularly sedation-related effects, were dose-related, transient, mild to moderate in severity, and did not interfere with attention or overall

  12. A genome-wide association study meta-analysis of clinical fracture in 10,012 African American women

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    Kira C. Taylor

    2016-12-01

    Full Text Available Background: Osteoporosis is a major public health problem associated with excess disability and mortality. It is estimated that 50–70% of the variation in osteoporotic fracture risk is attributable to genetic factors. The purpose of this hypothesis-generating study was to identify possible genetic determinants of fracture among African American (AA women in a GWAS meta-analysis. Methods: Data on clinical fractures (all fractures except fingers, toes, face, skull or sternum were analyzed among AA female participants in the Women's Health Initiative (WHI (N = 8155, Cardiovascular Health Study (CHS (N = 504, BioVU (N = 704, Health ABC (N = 651, and the Johnston County Osteoarthritis Project (JoCoOA (N = 291. Affymetrix (WHI and Illumina (Health ABC, JoCoOA, BioVU, CHS GWAS panels were used for genotyping, and a 1:1 ratio of YRI:CEU HapMap haplotypes was used as an imputation reference panel. We used Cox proportional hazard models or logistic regression to evaluate the association of ~2.5 million SNPs with fracture risk, adjusting for ancestry, age, and geographic region where applicable. We conducted a fixed-effects, inverse variance-weighted meta-analysis. Genome-wide significance was set at P < 5 × 10−8. Results: One SNP, rs12775980 in an intron of SVIL on chromosome 10p11.2, reached genome-wide significance (P = 4.0 × 10−8. Although this SNP has a low minor allele frequency (0.03, there was no evidence for heterogeneity of effects across the studies (I2 = 0. This locus was not reported in any previous osteoporosis-related GWA studies. We also interrogated previously reported GWA-significant loci associated with fracture or bone mineral density in our data. One locus (SMOC1 generalized, but overall there was not substantial evidence of generalization. Possible reasons for the lack of generalization are discussed. Conclusion: This GWAS meta-analysis of fractures in African American women identified a potentially novel

  13. Mutational analysis of the major soybean UreF paralogue involved in urease activation

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    Polacco, Joe C.; Hyten, David L.; Medeiros-Silva, Mônica; Sleper, David A.; Bilyeu, Kristin D.

    2011-01-01

    The soybean genome duplicated ∼14 and 45 million years ago and has many paralogous genes, including those in urease activation (emplacement of Ni and CO2 in the active site). Activation requires the UreD and UreF proteins, each encoded by two paralogues. UreG, a third essential activation protein, is encoded by the single-copy Eu3, and eu3 mutants lack activity of both urease isozymes. eu2 has the same urease-negative phenotype, consistent with Eu2 being a single-copy gene, possibly encoding a Ni carrier. Unexpectedly, two eu2 alleles co-segregated with missense mutations in the chromosome 2 UreF paralogue (Ch02UreF), suggesting lack of expression/function of Ch14UreF. However, Ch02UreF and Ch14UreF transcripts accumulate at the same level. Further, it had been shown that expression of the Ch14UreF ORF complemented a fungal ureF mutant. A third, nonsense (Q2*) allelic mutant, eu2-c, exhibited 5- to 10-fold more residual urease activity than missense eu2-a or eu2-b, though eu2-c should lack all Ch02UreF protein. It is hypothesized that low-level activation by Ch14UreF is ‘spoiled’ by the altered missense Ch02UreF proteins (‘epistatic dominant-negative’). In agreement with active ‘spoiling’ by eu2-b-encoded Ch02UreF (G31D), eu2-b/eu2-c heterozygotes had less than half the urease activity of eu2-c/eu2-c siblings. Ch02UreF (G31D) could spoil activation by Chr14UreF because of higher affinity for the activation complex, or because Ch02UreF (G31D) is more abundant than Ch14UreF. Here, the latter is favoured, consistent with a reported in-frame AUG in the 5' leader of Chr14UreF transcript. Translational inhibition could represent a form of ‘functional divergence’ of duplicated genes. PMID:21430294

  14. Accuracy of CNV Detection from GWAS Data.

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    Dandan Zhang

    2011-01-01

    Full Text Available Several computer programs are available for detecting copy number variants (CNVs using genome-wide SNP arrays. We evaluated the performance of four CNV detection software suites--Birdsuite, Partek, HelixTree, and PennCNV-Affy--in the identification of both rare and common CNVs. Each program's performance was assessed in two ways. The first was its recovery rate, i.e., its ability to call 893 CNVs previously identified in eight HapMap samples by paired-end sequencing of whole-genome fosmid clones, and 51,440 CNVs identified by array Comparative Genome Hybridization (aCGH followed by validation procedures, in 90 HapMap CEU samples. The second evaluation was program performance calling rare and common CNVs in the Bipolar Genome Study (BiGS data set (1001 bipolar cases and 1033 controls, all of European ancestry as measured by the Affymetrix SNP 6.0 array. Accuracy in calling rare CNVs was assessed by positive predictive value, based on the proportion of rare CNVs validated by quantitative real-time PCR (qPCR, while accuracy in calling common CNVs was assessed by false positive/false negative rates based on qPCR validation results from a subset of common CNVs. Birdsuite recovered the highest percentages of known HapMap CNVs containing >20 markers in two reference CNV datasets. The recovery rate increased with decreased CNV frequency. In the tested rare CNV data, Birdsuite and Partek had higher positive predictive values than the other software suites. In a test of three common CNVs in the BiGS dataset, Birdsuite's call was 98.8% consistent with qPCR quantification in one CNV region, but the other two regions showed an unacceptable degree of accuracy. We found relatively poor consistency between the two "gold standards," the sequence data of Kidd et al., and aCGH data of Conrad et al. Algorithms for calling CNVs especially common ones need substantial improvement, and a "gold standard" for detection of CNVs remains to be established.

  15. The effect of inversion at 8p23 on BLK association with lupus in Caucasian population.

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    Bahram Namjou

    Full Text Available To explore the potential influence of the polymorphic 8p23.1 inversion on known autoimmune susceptibility risk at or near BLK locus, we validated a new bioinformatics method that utilizes SNP data to enable accurate, high-throughput genotyping of the 8p23.1 inversion in a Caucasian population.Principal components analysis (PCA was performed using markers inside the inversion territory followed by k-means cluster analyses on 7416 European derived and 267 HapMaP CEU and TSI samples. A logistic regression conditional analysis was performed.Three subgroups have been identified; inversion homozygous, heterozygous and non-inversion homozygous. The status of inversion was further validated using HapMap samples that had previously undergone Fluorescence in situ hybridization (FISH assays with a concordance rate of above 98%. Conditional analyses based on the status of inversion were performed. We found that overall association signals in the BLK region remain significant after controlling for inversion status. The proportion of lupus cases and controls (cases/controls in each subgroup was determined to be 0.97 for the inverted homozygous group (1067 cases and 1095 controls, 1.12 for the inverted heterozygous group (1935 cases 1717 controls and 1.36 for non-inverted subgroups (924 cases and 678 controls. After calculating the linkage disequilibrium between inversion status and lupus risk haplotype we found that the lupus risk haplotype tends to reside on non-inversion background. As a result, a new association effect between non-inversion status and lupus phenotype has been identified ((p = 8.18×10(-7, OR = 1.18, 95%CI = 1.10-1.26.Our results demonstrate that both known lupus risk haplotype and inversion status act additively in the pathogenesis of lupus. Since inversion regulates expression of many genes in its territory, altered expression of other genes might also be involved in the development of lupus.

  16. Paenibacillus larvae 16S-23S rDNA intergenic transcribed spacer (ITS) regions: DNA fingerprinting and characterization.

    Science.gov (United States)

    Dingman, Douglas W

    2012-07-01

    Paenibacillus larvae is the causative agent of American foulbrood in honey bee (Apis mellifera) larvae. PCR amplification of the 16S-23S ribosomal DNA (rDNA) intergenic transcribed spacer (ITS) regions, and agarose gel electrophoresis of the amplified DNA, was performed using genomic DNA collected from 134 P. larvae strains isolated in Connecticut, six Northern Regional Research Laboratory stock strains, four strains isolated in Argentina, and one strain isolated in Chile. Following electrophoresis of amplified DNA, all isolates exhibited a common migratory profile (i.e., ITS-PCR fingerprint pattern) of six DNA bands. This profile represented a unique ITS-PCR DNA fingerprint that was useful as a fast, simple, and accurate procedure for identification of P. larvae. Digestion of ITS-PCR amplified DNA, using mung bean nuclease prior to electrophoresis, characterized only three of the six electrophoresis bands as homoduplex DNA and indicating three true ITS regions. These three ITS regions, DNA migratory band sizes of 915, 1010, and 1474 bp, signify a minimum of three types of rrn operons within P. larvae. DNA sequence analysis of ITS region DNA, using P. larvae NRRL B-3553, identified the 3' terminal nucleotides of the 16S rRNA gene, 5' terminal nucleotides of the 23S rRNA gene, and the complete DNA sequences of the 5S rRNA, tRNA(ala), and tRNA(ile) genes. Gene organization within the three rrn operon types was 16S-23S, 16S-tRNA(ala)-23S, and l6S-5S-tRNA(ile)-tRNA(ala)-23S and these operons were named rrnA, rrnF, and rrnG, respectively. The 23S rRNA gene was shown by I-CeuI digestion and pulsed-field gel electrophoresis of genomic DNA to be present as seven copies. This was suggestive of seven rrn operon copies within the P. larvae genome. Investigation of the 16S-23S rDNA regions of this bacterium has aided the development of a diagnostic procedure and has helped genomic mapping investigations via characterization of the ITS regions. Copyright © 2012 Elsevier Inc

  17. Selecting SNPs informative for African, American Indian and European Ancestry: application to the Family Investigation of Nephropathy and Diabetes (FIND).

    Science.gov (United States)

    Williams, Robert C; Elston, Robert C; Kumar, Pankaj; Knowler, William C; Abboud, Hanna E; Adler, Sharon; Bowden, Donald W; Divers, Jasmin; Freedman, Barry I; Igo, Robert P; Ipp, Eli; Iyengar, Sudha K; Kimmel, Paul L; Klag, Michael J; Kohn, Orly; Langefeld, Carl D; Leehey, David J; Nelson, Robert G; Nicholas, Susanne B; Pahl, Madeleine V; Parekh, Rulan S; Rotter, Jerome I; Schelling, Jeffrey R; Sedor, John R; Shah, Vallabh O; Smith, Michael W; Taylor, Kent D; Thameem, Farook; Thornley-Brown, Denyse; Winkler, Cheryl A; Guo, Xiuqing; Zager, Phillip; Hanson, Robert L

    2016-05-04

    The presence of population structure in a sample may confound the search for important genetic loci associated with disease. Our four samples in the Family Investigation of Nephropathy and Diabetes (FIND), European Americans, Mexican Americans, African Americans, and American Indians are part of a genome- wide association study in which population structure might be particularly important. We therefore decided to study in detail one component of this, individual genetic ancestry (IGA). From SNPs present on the Affymetrix 6.0 Human SNP array, we identified 3 sets of ancestry informative markers (AIMs), each maximized for the information in one the three contrasts among ancestral populations: Europeans (HAPMAP, CEU), Africans (HAPMAP, YRI and LWK), and Native Americans (full heritage Pima Indians). We estimate IGA and present an algorithm for their standard errors, compare IGA to principal components, emphasize the importance of balancing information in the ancestry informative markers (AIMs), and test the association of IGA with diabetic nephropathy in the combined sample. A fixed parental allele maximum likelihood algorithm was applied to the FIND to estimate IGA in four samples: 869 American Indians; 1385 African Americans; 1451 Mexican Americans; and 826 European Americans. When the information in the AIMs is unbalanced, the estimates are incorrect with large error. Individual genetic admixture is highly correlated with principle components for capturing population structure. It takes ~700 SNPs to reduce the average standard error of individual admixture below 0.01. When the samples are combined, the resulting population structure creates associations between IGA and diabetic nephropathy. The identified set of AIMs, which include American Indian parental allele frequencies, may be particularly useful for estimating genetic admixture in populations from the Americas. Failure to balance information in maximum likelihood, poly-ancestry models creates biased

  18. Identification of polymorphic inversions from genotypes

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    Cáceres Alejandro

    2012-02-01

    Full Text Available Abstract Background Polymorphic inversions are a source of genetic variability with a direct impact on recombination frequencies. Given the difficulty of their experimental study, computational methods have been developed to infer their existence in a large number of individuals using genome-wide data of nucleotide variation. Methods based on haplotype tagging of known inversions attempt to classify individuals as having a normal or inverted allele. Other methods that measure differences between linkage disequilibrium attempt to identify regions with inversions but unable to classify subjects accurately, an essential requirement for association studies. Results We present a novel method to both identify polymorphic inversions from genome-wide genotype data and classify individuals as containing a normal or inverted allele. Our method, a generalization of a published method for haplotype data 1, utilizes linkage between groups of SNPs to partition a set of individuals into normal and inverted subpopulations. We employ a sliding window scan to identify regions likely to have an inversion, and accumulation of evidence from neighboring SNPs is used to accurately determine the inversion status of each subject. Further, our approach detects inversions directly from genotype data, thus increasing its usability to current genome-wide association studies (GWAS. Conclusions We demonstrate the accuracy of our method to detect inversions and classify individuals on principled-simulated genotypes, produced by the evolution of an inversion event within a coalescent model 2. We applied our method to real genotype data from HapMap Phase III to characterize the inversion status of two known inversions within the regions 17q21 and 8p23 across 1184 individuals. Finally, we scan the full genomes of the European Origin (CEU and Yoruba (YRI HapMap samples. We find population-based evidence for 9 out of 15 well-established autosomic inversions, and for 52 regions

  19. Congruence as a measurement of extended haplotype structure across the genome

    Science.gov (United States)

    2012-01-01

    Background Historically, extended haplotypes have been defined using only a few data points, such as alleles for several HLA genes in the MHC. High-density SNP data, and the increasing affordability of whole genome SNP typing, creates the opportunity to define higher resolution extended haplotypes. This drives the need for new tools that support quantification and visualization of extended haplotypes as defined by as many as 2000 SNPs. Confronted with high-density SNP data across the major histocompatibility complex (MHC) for 2,300 complete families, compiled by the Type 1 Diabetes Genetics Consortium (T1DGC), we developed software for studying extended haplotypes. Methods The software, called ExHap (Extended Haplotype), uses a similarity measurement we term congruence to identify and quantify long-range allele identity. Using ExHap, we analyzed congruence in both the T1DGC data and family-phased data from the International HapMap Project. Results Congruent chromosomes from the T1DGC data have between 96.5% and 99.9% allele identity over 1,818 SNPs spanning 2.64 megabases of the MHC (HLA-DRB1 to HLA-A). Thirty-three of 132 DQ-DR-B-A defined haplotype groups have > 50% congruent chromosomes in this region. For example, 92% of chromosomes within the DR3-B8-A1 haplotype are congruent from HLA-DRB1 to HLA-A (99.8% allele identity). We also applied ExHap to all 22 autosomes for both CEU and YRI cohorts from the International HapMap Project, identifying multiple candidate extended haplotypes. Conclusions Long-range congruence is not unique to the MHC region. Patterns of allele identity on phased chromosomes provide a simple, straightforward approach to visually and quantitatively inspect complex long-range structural patterns in the genome. Such patterns aid the biologist in appreciating genetic similarities and differences across cohorts, and can lead to hypothesis generation for subsequent studies. PMID:22369243

  20. Determination of sites of U50,488H-promoted phosphorylation of the mouse κ opioid receptor (KOPR): disconnect between KOPR phosphorylation and internalization.

    Science.gov (United States)

    Chen, Chongguang; Chiu, Yi-Ting; Wu, Wenman; Huang, Peng; Mann, Anika; Schulz, Stefan; Liu-Chen, Lee-Yuan

    2016-02-15

    Phosphorylation sites of KOPR (κ opioid receptor) following treatment with the selective agonist U50,488H {(-)(trans)-3,4-dichloro-N-methyl-N-[2-(1-pyrrolidiny)cyclo-hexyl]benzeneacetamide} were identified after affinity purification, SDS/PAGE, in-gel digestion with Glu-C and HPLC-MS/MS. Single- and double-phosphorylated peptides were identified containing phosphorylated Ser(356), Thr(357), Thr(363) and Ser(369) in the C-terminal domain. Antibodies were generated against three phosphopeptides containing pSer(356)/pThr(357), pThr(363) and pSer(369) respectively, and affinity-purified antibodies were found to be highly specific for phospho-KOPR. U50,488H markedly enhanced staining of the KOPR by pThr(363)-, pSer(369)- and pSer(356)/pThr(357)-specific antibodies in immunoblotting, which was blocked by the selective KOPR antagonist norbinaltorphimine. Ser(369) phosphorylation affected Thr(363) phosphorylation and vice versa, and Thr(363) or Ser(369) phosphorylation was important for Ser(356)/Thr(357) phosphorylation, revealing a phosphorylation hierarchy. U50,488H, but not etorphine, promoted robust KOPR internalization, although both were full agonists. U50,488H induced higher degrees of phosphorylation than etorphine at Ser(356)/Thr(357), Thr(363) and Ser(369) as determined by immunoblotting. Using SILAC (stable isotope labelling by amino acids in cell culture) and HPLC-MS/MS, we found that, compared with control (C), U50,488H (U) and etorphine (E) KOPR promoted single phosphorylation primarily at Thr(363) and Ser(369) with U/E ratios of 2.5 and 2 respectively. Both induced double phosphorylation at Thr(363)+Ser(369) and Thr(357)+Ser(369) with U/E ratios of 3.3 and 3.4 respectively. Only U50,488H induced triple phosphorylation at Ser(356)+Thr(357)+Ser(369). An unphosphorylated KOPR-(354-372) fragment containing all of the phosphorylation sites was detected with a C/E/U ratio of 1/0.7/0.4, indicating that ∼60% and ∼30% of the mouse KOPR are phosphorylated

  1. Atypical epidemiology of CTX-M-15 among Enterobacteriaceae from a high diversity of non-clinical niches in Angola.

    Science.gov (United States)

    Ribeiro, T G; Novais, Â; Peixe, L; Machado, E

    2016-05-01

    The objective of this study was to investigate the distribution and molecular epidemiology of ESBLs, acquired AmpCs and carbapenemases in Enterobacteriaceae from non-clinical niches in Angola, an under-researched sub-Saharan country. Eighty-one samples were recovered from healthy persons (n = 18), healthy animals (n = 33) and their environments (n = 10) or aquatic settings (n = 20) in south Angola (2013). Samples were plated onto CHROMagar™ Orientation with/without antibiotics. Standard methods were used for bacterial identification, characterization of bla genes, antibiotic susceptibility testing and conjugation assays. Clonal analysis (XbaI-PFGE, MLST and Escherichia coli phylogroups), location of bla and plasmid characterization (S1-PFGE, I-CeuI-PFGE, replicon typing and hybridization) were also performed. ESBLs (almost exclusively CTX-M-15, 98%) were detected in 21% (45/216) of the isolates, recovered from diverse non-clinical niches and belonging to different Enterobacteriaceae species (mainly E. coli). Acquired AmpCs or carbapenemases were not found. The pandemic B2-ST131 E. coli clone was not identified, but some widespread clonal complexes (CCs) from A (CC10 and CC168), B1 (CC156) or D (CC38) phylogroups were detected. blaCTX-M-15 was variably identified on typeable (29%; 100-335 kb; IncFII, IncFIIK6, IncHI2 and IncY) or non-typeable (16%; 70-330 kb) plasmids or on the chromosome (14%), while for 41% of the isolates its specific location was not determined. This study reports, for the first time in Angola, an unexpected high occurrence of CTX-M-15 in diverse non-clinical niches and Enterobacteriaceae species, and uncovers novel plasmid replicons in under-researched geographical regions. The diffusion of blaCTX-M-15 through such a high diversity of genetic backgrounds (clones, typeable/non-typeable plasmids and genetic environments) unveils an extraordinary ability for blaCTX-M-15 acquisition and mobilization favoured by unrecognized

  2. Anemia e renda per capita familiar de crianças freqüentadoras da creche do Centro Educacional Unificado Cidade Dutra, no Município de São Paulo Anemia and per capita income in children enrolled in a childhood education center in São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Teresinha Stumpf Souto

    2007-06-01

    Full Text Available OBJETIVO: Avaliar a freqüência de anemia de acordo com a renda familiar per capita de crianças matriculadas no Centro de Educação Infantil (CEI do Centro Educacional Unificado (CEU Cidade Dutra, no Município de São Paulo, São Paulo, em 2004. MÉTODOS: Estudo transversal, com 190 crianças de 11 a 57 meses de idade distribuídas em duas faixas de renda familiar per capita ( 1 salário mínimo. Foram consideradas anêmicas as crianças com taxa de hemoglobina OBJECTIVE: To evaluate the frequency of anemia according to family per capita income in children enrolled in a day care center in São Paulo, Brazil, in 2004. METHODS: This cross-sectional study enrolled 190 children aged 11 to 57 months divided into two groups of per capita income ( 1 minimum wage. Hemoglobin level was evaluated in capillary blood (finger-stick test using a Hemocue® portable photometer. Anemia was considered when hemoglobin level was below 11g/dL. The results were analyzed using chi-square and Kruskal-Wallis tests. RESULTS: Anemia was found in 31.6% of the investigated children, with a higher proportion in younger children. The frequency of anemia was, respectively, 72.7%, 41.2%, 19.7% and 15.4% in children aged 11-23 months age, 24-35 months, 36-47 months, and 48-57 months. Among children of families with low per capita income, the frequency of anemia was 36.7%. In families earning more than one minimum wage per capita per month, there were 22.9% of children with anemia. CONCLUSIONS: At the day care center, the frequency of anemia was higher among children aged 11-23 months, especially when the family per capita income was less than one minimum wage per month.

  3. Prevalence and characterization of Campylobacter jejuni isolated from pasture flock poultry.

    Science.gov (United States)

    Hanning, Irene; Biswas, Debabrata; Herrera, Paul; Roesler, Mary; Ricke, Steven C

    2010-09-01

    The growing interest in organic and natural foods warrants a greater need for information on the food safety of these products. In this study, samples were taken from 2 pasture flock farms (N = 178; feed, water, drag swabs, and insect traps), pasture flock retail carcasses (N = 48) and 1 pasture flock processing facility (N = 16) over a period of 8 mo. A total of 105 Campylobacter isolates were obtained from 53 (30%), 36 (75%), and 16 (100%) samples from the farms, retail carcasses, and processing facility, respectively. Of the 105 isolates collected, 65 were C. jejuni, 31 were C. coli, and 9 were other Campylobacter spp. Using PCR, the C. jejuni isolates were further analyzed for virulence genes involved in colonization and survival (flaA, flaC, cadF, dnaJ, racR, cbrR), invasion (virB11, ciaB, pldA), protection against harsh conditions (sodB, htrA, clpA), toxin production (cdtA, cdtB, cdtC), siderophore transport (ceuE), and ganglioside mimicry (wlaN). In addition, the short variable region of the flaA locus (flaA SVR) was sequenced to determine the genetic diversity of the C. jejuni isolates. The flaA SVR diversity indices increased along the farm to carcass continuum. PCR-based analysis indicated a low prevalence of 5 genes involved in colonization (dnaJ, ciaB, pldA, racR, virB11). The results of this survey indicate that the prevalence of Campylobacter on organic retail carcasses is similar to prevalence reports of Campylobacter on conventional retail carcasses. However, the genetic diversity of the flaA SVR genotypes increased along the farm to carcass continuum that contrasted with conventional poultry studies. Campylobacter jejuni is a leading cause of foodborne illness with poultry and poultry products being leading sources of infection. Free-range and pasture flock chickens are becoming more popular; however, there is an inherent biosecurity risk that can increase the prevalence of foodborne pathogens in these flocks. This study aimed to determine sources

  4. Calendar

    Science.gov (United States)

    2004-04-01

    2004 dkermani@fit.edu, +1 321 674 7412, http://kermani.math.fit.edu/ JULY 2004 2nd World Congress of the Game Theory Society, Faculty of Luminy, Marseille, France 5-9 July 2004 Europa Organisation (europa@europa-organisation.com), +33 5 34 45 26 45, www.gts2004.org Budapest Workshop on Behavioral Economics, Central European University, Budapest, Hungary 5-10 July 2004 Eva Dotzi (behavecon@ceu.hu), www.iza.org/en/calls_conferences/CallCEU_04.pdf FDA'04 1st IFAC Workshop on Fractional Differentiation and its Applications, Bordeaux, France 19-20 July 2004 IFAC secretariat (fda04@lap.u-bordeaux1.fr), www.lap.u-bordeaux.fr/fda04/ Bachelier Finance Society Third World Congress, InterContinental Hotel, Chicago, IL, USA 21-24 July 2004 bfs2004@uic.edu, www.uic.edu/orgs/bachelier/ BS/IMS 2004 6th World Congress of the Bernoulli Society for Mathematical Statistics and Probability, Barcelona, Spain 26-31 July 2004 wc2004@pacifico-meetings.com, +34 93 402 13 85, www.imub.ub.es/events/wc2004 AUGUST 2004 Summer School in Econometrics. The Cointegrated VAR Model: Econometric Methodology and Macroeconomic Applications, Institute of Economics, University of Copenhagen, Denmark 2-22 August 2004 Summerschool@econ.ku.dk, www.econ.ku.dk/summerschool SEPTEMBER 2004 First Bonzenfreies Colloquium on Market Dynamics and Quantitative Economics, Alessandria, Palazzo Borsalino, Italy 9-10 September 2004 colloquium@unipmn.it, www.mfn.unipmn.it/~colloqui/ Risk Analysis 2004. 4th International Conference on Computer Simulation in Risk Analysis and Hazard Mitigation, Aldemar Paradise Royal Mare Hotel, Rhodes, Greece 27-29 September 2004 enquiries@wessex.ac.uk, +44 (0)238 029 3223, www.wessex.ac.uk/conferences/2004/risk04/ OCTOBER 2004 IRC Hedge 2004, InterContinental Hotel, London, UK 10, 11 October 2004 enquiries@irc-conferences.com, www.irc-conferences.com/show_conference.php?id=10 NOVEMBER 2004 IRC DICE 2004, InterContinental Hotel, London, UK 22, 23 November 2004 enquiries

  5. Design and Testing of a Controller for the Martian Atmosphere Pressure and Humidity Instrument DREAMS-P/H

    Science.gov (United States)

    Tapani Nikkanen, Timo; Schmidt, Walter; Genzer, Maria; Harri, Ari-Matti; Haukka, Harri

    2013-04-01

    The European Space Agency (ESA), driven by the goal of performing a soft landing on Mars, is planning to launch the Entry, descent and landing Demonstrator Module (EDM)[1] simultaneously with the Trace Gas Orbiter (TGO) as a part of the ExoMars program towards Mars in 2016. As a secondary objective, the EDM will gather meteorological data and observe the electrical environment of the landing site with its Dust characterisation, Risk assessment, and Environmental Analyser on the Martian Surface (DREAMS). The Finnish Meteorological Institute (FMI) is participating in the project by designing, building and testing a pressure and a humidity instrument for Mars, named DREAMS-P and DREAMS-H, respectively. The instruments are based on previous FMI designs, including ones flown on board the Huygens, Phoenix and Mars Science Laboratory.[2] Traditionally, the FMI pressure and humidity instruments have been controlled by an FPGA. However, the need to incorporate more autonomy and modifiability into instruments, cut the development time and component costs, stimulated interest to study a Commercial Off-The-Shelf (COTS) Microcontroller Unit (MCU) based instrument design. Thus, in the DREAMS-P/H design, an automotive MCU is used as the instrument controller. The MCU has been qualified for space by tests in and outside FMI. The DREAMS-P/H controller command and data interface utilizes a RS-422 connection to receive telecommands from and to transmit data to the Central Electronics Unit (CEU) of the DREAMS science package. The two pressure transducers of DREAMS-P and one humidity transducer of DREAMS-H are controlled by a single MCU. The MCU controls the power flow for each transducer and performs pulse counting measurements on sensor and reference channels to retrieve scientific data. Pressure and humidity measurements are scheduled and set up according to a configuration table assigned to each transducer. The configuration tables can be modified during the flight. The whole

  6. Accuracy of genome-wide imputation of untyped markers and impacts on statistical power for association studies

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    McElwee Joshua

    2009-06-01

    Full Text Available Abstract Background Although high-throughput genotyping arrays have made whole-genome association studies (WGAS feasible, only a small proportion of SNPs in the human genome are actually surveyed in such studies. In addition, various SNP arrays assay different sets of SNPs, which leads to challenges in comparing results and merging data for meta-analyses. Genome-wide imputation of untyped markers allows us to address these issues in a direct fashion. Methods 384 Caucasian American liver donors were genotyped using Illumina 650Y (Ilmn650Y arrays, from which we also derived genotypes from the Ilmn317K array. On these data, we compared two imputation methods: MACH and BEAGLE. We imputed 2.5 million HapMap Release22 SNPs, and conducted GWAS on ~40,000 liver mRNA expression traits (eQTL analysis. In addition, 200 Caucasian American and 200 African American subjects were genotyped using the Affymetrix 500 K array plus a custom 164 K fill-in chip. We then imputed the HapMap SNPs and quantified the accuracy by randomly masking observed SNPs. Results MACH and BEAGLE perform similarly with respect to imputation accuracy. The Ilmn650Y results in excellent imputation performance, and it outperforms Affx500K or Ilmn317K sets. For Caucasian Americans, 90% of the HapMap SNPs were imputed at 98% accuracy. As expected, imputation of poorly tagged SNPs (untyped SNPs in weak LD with typed markers was not as successful. It was more challenging to impute genotypes in the African American population, given (1 shorter LD blocks and (2 admixture with Caucasian populations in this population. To address issue (2, we pooled HapMap CEU and YRI data as an imputation reference set, which greatly improved overall performance. The approximate 40,000 phenotypes scored in these populations provide a path to determine empirically how the power to detect associations is affected by the imputation procedures. That is, at a fixed false discovery rate, the number of cis

  7. SU-CD-PinS Room/Hall E-01

    International Nuclear Information System (INIS)

    Parker, R.

    2016-01-01

    excepted packages and Type A packages will be emphasized. The program is designed to meet the function specific DOT training requirements for shippers of medical radioactive materials. General awareness training and security awareness training can be obtained from two free DOT training CDs. Safety training and security awareness training is generally satisfied by the training required under the institution’s radioactive material license. For shippers of radioactive Yellow III labeled packages an in-depth written security plan and training are no longer required as of April 8, 2010. In general almost all shippers of medical radioactive material are now not required to have an in-depth security plan. Contents of general awareness training, security awareness training and in-depth security plans will be briefly outlined. It is the hazmat employer’s responsibility to ensure that each hazmat employee is properly trained. No third party can fulfill that requirement. It is the hazmat employer’s responsibility to determine the degree to which this course meets the employer’s requirements, including contents of the course and the examination. Participants will gain sufficient knowledge to prepare hazmat training programs for others in their institutions. A handout will be posted which should be printed out and brought to the course for reference during the presentation. The handout will also satisfy part of the training documentation required by DOT. A feature handout section is a composite table which provides A1, A2, RQ, Exempt Concentration, and Exempt Consignment values in a single table in both Becquerel and Curie units. Course attendance will be certified through the AAPM CEU documentation system. Learning Objectives: Understand the regulatory requirements for shipping radioactive materials. Understand the regulatory requirements for training of hazmat employees. Comprehend how to classify, package, mark, label, document, placard, and transport radioactive materials.

  8. Relationships between craniocervical posture and pain-related disability in patients with cervico-craniofacial pain

    Directory of Open Access Journals (Sweden)

    López-de-Uralde-Villanueva I

    2015-07-01

    Full Text Available Ibai López-de-Uralde-Villanueva,1–4 Hector Beltran-Alacreu,1–3 Alba Paris-Alemany,1–4 Santiago Angulo-Díaz-Parreño,2,3,5 Roy La Touche1–4 1Department of Physiotherapy, Faculty of Health Science, 2Research Group on Movement and Behavioral Science and Study of Pain, The Center for Advanced Studies University La Salle, Universidad Autónoma de Madrid, Aravaca, Madrid, Spain; 3Institute of Neuroscience and Craniofacial Pain (INDCRAN, Madrid, Spain; 4Hospital La Paz Institute for Health Research, IdiPAZ, Madrid, Spain; 5Faculty of Medicine, Universidad San Pablo CEU, Madrid, Spain Objectives: This cross-sectional correlation study explored the relationships between craniocervical posture and pain-related disability in patients with chronic cervico-craniofacial pain (CCFP. Moreover, we investigated the test–retest intrarater reliability of two craniocervical posture measurements: head posture (HP and the sternomental distance (SMD. Methods: Fifty-three asymptomatic subjects and 60 CCFP patients were recruited. One rater measured HP and the SMD using a cervical range of motion device and a digital caliper, respectively. The Spanish versions of the neck disability index and the craniofacial pain and disability inventory were used to assess pain-related disability (neck disability and craniofacial disability, respectively. Results: We found no statistically significant correlations between craniocervical posture and pain-related disability variables (HP and neck disability [r=0.105; P>0.05]; HP and craniofacial disability [r=0.132; P>0.05]; SMD and neck disability [r=0.126; P>0.05]; SMD and craniofacial disability [r=0.195; P>0.05]. A moderate positive correlation was observed between HP and SMD for both groups (asymptomatic subjects, r=0.447; CCFP patients, r=0.52. Neck disability was strongly positively correlated with craniofacial disability (r=0.79; P>0.001. The test–retest intrarater reliability of the HP measurement was high for

  9. SU-G-PinS Room/Hall E-01

    International Nuclear Information System (INIS)

    Parker, R.

    2016-01-01

    excepted packages and Type A packages will be emphasized. The program is designed to meet the function specific DOT training requirements for shippers of medical radioactive materials. General awareness training and security awareness training can be obtained from two free DOT training CDs. Safety training and security awareness training is generally satisfied by the training required under the institution’s radioactive material license. For shippers of radioactive Yellow III labeled packages an in-depth written security plan and training are no longer required as of April 8, 2010. In general almost all shippers of medical radioactive material are now not required to have an in-depth security plan. Contents of general awareness training, security awareness training and in-depth security plans will be briefly outlined. It is the hazmat employer’s responsibility to ensure that each hazmat employee is properly trained. No third party can fulfill that requirement. It is the hazmat employer’s responsibility to determine the degree to which this course meets the employer’s requirements, including contents of the course and the examination. Participants will gain sufficient knowledge to prepare hazmat training programs for others in their institutions. A handout will be posted which should be printed out and brought to the course for reference during the presentation. The handout will also satisfy part of the training documentation required by DOT. A feature handout section is a composite table which provides A1, A2, RQ, Exempt Concentration, and Exempt Consignment values in a single table in both Becquerel and Curie units. Course attendance will be certified through the AAPM CEU documentation system. Learning Objectives: Understand the regulatory requirements for shipping radioactive materials. Understand the regulatory requirements for training of hazmat employees. Comprehend how to classify, package, mark, label, document, placard, and transport radioactive materials.

  10. SU-CD-PinS Room/Hall E-00: HAZMAT Training for the Medical Physicist - Part I

    International Nuclear Information System (INIS)

    2016-01-01

    excepted packages and Type A packages will be emphasized. The program is designed to meet the function specific DOT training requirements for shippers of medical radioactive materials. General awareness training and security awareness training can be obtained from two free DOT training CDs. Safety training and security awareness training is generally satisfied by the training required under the institution’s radioactive material license. For shippers of radioactive Yellow III labeled packages an in-depth written security plan and training are no longer required as of April 8, 2010. In general almost all shippers of medical radioactive material are now not required to have an in-depth security plan. Contents of general awareness training, security awareness training and in-depth security plans will be briefly outlined. It is the hazmat employer’s responsibility to ensure that each hazmat employee is properly trained. No third party can fulfill that requirement. It is the hazmat employer’s responsibility to determine the degree to which this course meets the employer’s requirements, including contents of the course and the examination. Participants will gain sufficient knowledge to prepare hazmat training programs for others in their institutions. A handout will be posted which should be printed out and brought to the course for reference during the presentation. The handout will also satisfy part of the training documentation required by DOT. A feature handout section is a composite table which provides A1, A2, RQ, Exempt Concentration, and Exempt Consignment values in a single table in both Becquerel and Curie units. Course attendance will be certified through the AAPM CEU documentation system. Learning Objectives: Understand the regulatory requirements for shipping radioactive materials. Understand the regulatory requirements for training of hazmat employees. Comprehend how to classify, package, mark, label, document, placard, and transport radioactive materials.

  11. SU-G-PinS Room/Hall E-00: HAZMAT Training for the Medical Physicist - Part II

    International Nuclear Information System (INIS)

    2016-01-01

    excepted packages and Type A packages will be emphasized. The program is designed to meet the function specific DOT training requirements for shippers of medical radioactive materials. General awareness training and security awareness training can be obtained from two free DOT training CDs. Safety training and security awareness training is generally satisfied by the training required under the institution’s radioactive material license. For shippers of radioactive Yellow III labeled packages an in-depth written security plan and training are no longer required as of April 8, 2010. In general almost all shippers of medical radioactive material are now not required to have an in-depth security plan. Contents of general awareness training, security awareness training and in-depth security plans will be briefly outlined. It is the hazmat employer’s responsibility to ensure that each hazmat employee is properly trained. No third party can fulfill that requirement. It is the hazmat employer’s responsibility to determine the degree to which this course meets the employer’s requirements, including contents of the course and the examination. Participants will gain sufficient knowledge to prepare hazmat training programs for others in their institutions. A handout will be posted which should be printed out and brought to the course for reference during the presentation. The handout will also satisfy part of the training documentation required by DOT. A feature handout section is a composite table which provides A1, A2, RQ, Exempt Concentration, and Exempt Consignment values in a single table in both Becquerel and Curie units. Course attendance will be certified through the AAPM CEU documentation system. Learning Objectives: Understand the regulatory requirements for shipping radioactive materials. Understand the regulatory requirements for training of hazmat employees. Comprehend how to classify, package, mark, label, document, placard, and transport radioactive materials.

  12. Report on the International Workshop “Networks, Regions and Institutions in Mongol Eurasia: A Meso-Historical Analysis”, Jerusalem, 17–18 May, 2017

    Directory of Open Access Journals (Sweden)

    Roman Hautala

    2017-09-01

    Everyday Life in the Golden Horde (First Half of the 14th Century”; Stephen Pow, (CEU University “What a Source Says, When It Says Nothing at All: Extracting Information from Jochi Khan’s Biography in the 117th Chapter of the Yuan Shi”; Jonathan Brack (The Hebrew University of Jerusalem, “How the Dead Mattered in Ilkhanid Iran? The Local and the Global in Rashid al-Din’s Three Refutations of Reincarnation”. In the framework of the final fourth panel “Law as a Meso-Institution” the following talks were presented: Khohchahar E. Chuluu (The University of Tokyo, “Law, Institutions, and Justice in the Mongol Empire”; Florence Hodous (Renmin University, “Joint Trials as a Key to Local History and Empire-Wide Dynamics”; Edith Chen (Princeton University, “Justice of the Khan: Writing the Lives of the Jarqučis in the Yuan Shi”.

  13. SU-G-PinS Room/Hall E-01

    Energy Technology Data Exchange (ETDEWEB)

    Parker, R. [Department of Transportation Hazmat Employee Training for Shippers of Radioactive Materials (United States)

    2016-06-15

    excepted packages and Type A packages will be emphasized. The program is designed to meet the function specific DOT training requirements for shippers of medical radioactive materials. General awareness training and security awareness training can be obtained from two free DOT training CDs. Safety training and security awareness training is generally satisfied by the training required under the institution’s radioactive material license. For shippers of radioactive Yellow III labeled packages an in-depth written security plan and training are no longer required as of April 8, 2010. In general almost all shippers of medical radioactive material are now not required to have an in-depth security plan. Contents of general awareness training, security awareness training and in-depth security plans will be briefly outlined. It is the hazmat employer’s responsibility to ensure that each hazmat employee is properly trained. No third party can fulfill that requirement. It is the hazmat employer’s responsibility to determine the degree to which this course meets the employer’s requirements, including contents of the course and the examination. Participants will gain sufficient knowledge to prepare hazmat training programs for others in their institutions. A handout will be posted which should be printed out and brought to the course for reference during the presentation. The handout will also satisfy part of the training documentation required by DOT. A feature handout section is a composite table which provides A1, A2, RQ, Exempt Concentration, and Exempt Consignment values in a single table in both Becquerel and Curie units. Course attendance will be certified through the AAPM CEU documentation system. Learning Objectives: Understand the regulatory requirements for shipping radioactive materials. Understand the regulatory requirements for training of hazmat employees. Comprehend how to classify, package, mark, label, document, placard, and transport radioactive materials.

  14. SU-CD-PinS Room/Hall E-00: HAZMAT Training for the Medical Physicist - Part I

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2016-06-15

    excepted packages and Type A packages will be emphasized. The program is designed to meet the function specific DOT training requirements for shippers of medical radioactive materials. General awareness training and security awareness training can be obtained from two free DOT training CDs. Safety training and security awareness training is generally satisfied by the training required under the institution’s radioactive material license. For shippers of radioactive Yellow III labeled packages an in-depth written security plan and training are no longer required as of April 8, 2010. In general almost all shippers of medical radioactive material are now not required to have an in-depth security plan. Contents of general awareness training, security awareness training and in-depth security plans will be briefly outlined. It is the hazmat employer’s responsibility to ensure that each hazmat employee is properly trained. No third party can fulfill that requirement. It is the hazmat employer’s responsibility to determine the degree to which this course meets the employer’s requirements, including contents of the course and the examination. Participants will gain sufficient knowledge to prepare hazmat training programs for others in their institutions. A handout will be posted which should be printed out and brought to the course for reference during the presentation. The handout will also satisfy part of the training documentation required by DOT. A feature handout section is a composite table which provides A1, A2, RQ, Exempt Concentration, and Exempt Consignment values in a single table in both Becquerel and Curie units. Course attendance will be certified through the AAPM CEU documentation system. Learning Objectives: Understand the regulatory requirements for shipping radioactive materials. Understand the regulatory requirements for training of hazmat employees. Comprehend how to classify, package, mark, label, document, placard, and transport radioactive materials.

  15. SU-CD-PinS Room/Hall E-01

    Energy Technology Data Exchange (ETDEWEB)

    Parker, R.

    2016-06-15

    excepted packages and Type A packages will be emphasized. The program is designed to meet the function specific DOT training requirements for shippers of medical radioactive materials. General awareness training and security awareness training can be obtained from two free DOT training CDs. Safety training and security awareness training is generally satisfied by the training required under the institution’s radioactive material license. For shippers of radioactive Yellow III labeled packages an in-depth written security plan and training are no longer required as of April 8, 2010. In general almost all shippers of medical radioactive material are now not required to have an in-depth security plan. Contents of general awareness training, security awareness training and in-depth security plans will be briefly outlined. It is the hazmat employer’s responsibility to ensure that each hazmat employee is properly trained. No third party can fulfill that requirement. It is the hazmat employer’s responsibility to determine the degree to which this course meets the employer’s requirements, including contents of the course and the examination. Participants will gain sufficient knowledge to prepare hazmat training programs for others in their institutions. A handout will be posted which should be printed out and brought to the course for reference during the presentation. The handout will also satisfy part of the training documentation required by DOT. A feature handout section is a composite table which provides A1, A2, RQ, Exempt Concentration, and Exempt Consignment values in a single table in both Becquerel and Curie units. Course attendance will be certified through the AAPM CEU documentation system. Learning Objectives: Understand the regulatory requirements for shipping radioactive materials. Understand the regulatory requirements for training of hazmat employees. Comprehend how to classify, package, mark, label, document, placard, and transport radioactive materials.

  16. SU-G-PinS Room/Hall E-00: HAZMAT Training for the Medical Physicist - Part II

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2016-06-15

    excepted packages and Type A packages will be emphasized. The program is designed to meet the function specific DOT training requirements for shippers of medical radioactive materials. General awareness training and security awareness training can be obtained from two free DOT training CDs. Safety training and security awareness training is generally satisfied by the training required under the institution’s radioactive material license. For shippers of radioactive Yellow III labeled packages an in-depth written security plan and training are no longer required as of April 8, 2010. In general almost all shippers of medical radioactive material are now not required to have an in-depth security plan. Contents of general awareness training, security awareness training and in-depth security plans will be briefly outlined. It is the hazmat employer’s responsibility to ensure that each hazmat employee is properly trained. No third party can fulfill that requirement. It is the hazmat employer’s responsibility to determine the degree to which this course meets the employer’s requirements, including contents of the course and the examination. Participants will gain sufficient knowledge to prepare hazmat training programs for others in their institutions. A handout will be posted which should be printed out and brought to the course for reference during the presentation. The handout will also satisfy part of the training documentation required by DOT. A feature handout section is a composite table which provides A1, A2, RQ, Exempt Concentration, and Exempt Consignment values in a single table in both Becquerel and Curie units. Course attendance will be certified through the AAPM CEU documentation system. Learning Objectives: Understand the regulatory requirements for shipping radioactive materials. Understand the regulatory requirements for training of hazmat employees. Comprehend how to classify, package, mark, label, document, placard, and transport radioactive materials.

  17. CARACTERIZAÇÃO DE GRÃOS DE GIRASSOL (Hellianthus annuus L. AO LONGO DO PERÍODO DE COLHEITA EM DOIS MUNICÍPIOS DO ESTADO DE GOIÁS CHARACTERISTICS OF SUNFLOWER GRAINS DURING THE HARVEST PERIOD IN TWO COUNTIES OF THE STATE OF GOIÁS

    Directory of Open Access Journals (Sweden)

    Heloina Teresinha Faleiro

    2007-09-01

    ás is the major roducer, with 70.5% of the 1999 national production. The ultivation of the sunflower seed is becoming more important nd there are no studies about the quality of the grain. Therefore, here is a need to gather information about the characteristics of he grain harvested. The goal of this study is to collect data from wo storage units (Montividiu and Chapadão do Céu counties, hich receive most the state of Goiás’ production. The average oisture content, percentage of impurity and percentage of burned grains from Montividiu were 9%, 2.5% and 1%, and from hapadão do Céu were 10.3%, 4.1% and 3.3%, respectively. he temperature was between 17.5° and 22°C in the Montividiu nit and between 7° and 23.5°C in the Chapadão do Ceu unit. he relative humidity was between 37% and 71.5% in Montividiu nd between 38.5% and 80.5% in Chapadão do Céu. The grain ample collected showed 21.9% protein and 35.6% oil. In the ample, 790 CFU of fungi were encountered per gram of product, ith 86% Cladosporium fungus, 0.9% of Fusarium and 0.4% of Penicillium. One sample of sunflower residue had 8.6% humidity, 6.3% protein and 7.5% oil. In the counting and identification of fungi, 2.5 x 10³ CFU of fungi were encountered per gram of esidue, with 57% Fusarium fungus and 43% Penicillium fungus. he observed characteristics of moisture content, percentage of mpurity and percentage of burned grains are in accordance with uality standards.

    KEY-WORDS: Quality; classification; weather.

  18. Effective strategy making: Co-designing scenarios as a tool for effective strategic planning

    Directory of Open Access Journals (Sweden)

    Jan Vogelij

    2015-08-01

    level approaches the process positively and supportive, although without unconditional commitment to any outcome of the process. The empiric part contains case studies of five strategy making processes in different European countries. The selection of cases was based on a pre-selection proposed by planners, representing their countries in the European Council of Spatial Planners (ECTP-CEU They responded to the request for good examples of cases of successful strategy making in their countries. The final selection of five was chosen applying practical considerations about expectations of local help in assembling information. The selected cases were: Piano Strutturale Comunale of Bologna (IT, Scenariostudie Drechtsteden 2030 (NL, the Glasgow and the Clyde Valley Strategic Development Plan (UK, Vision Gherdëina (IT and Meetjesland 2020 (BE. Next to plan documents and existing evaluations, forty-one interviews formed important sources of information for connecting casuistic and theory. Applying our definition of effectiveness, first the effectiveness of our cases was established. Not all of those five appeared to be effective according to our criteria as formulated in the theoretical frame. Next the cases were analysed, using pre-formulated hypotheses for process and place-related conditions influencing the performance of spatial development strategies. The accounts of cases in different planning cultures related to the process and place-aspects of the theoretic frame provided insight in the different process aspects and the circumstances in which those processes were conducted. That, in combination with the earlier established (non- effectiveness enabled characterizing the processes within their various administrative and societal environments. Subsequently the process aspects and the place-related aspects of the five cases were compared and analysed applying the research questions, the hypotheses and the chains of evidence. Such comparison allowed for conclusions about