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Sample records for cervix uteri vagina

  1. The bovine cervix explored : the cow as a model for studies on functional changes in the cervix uteri

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    Breeveld-Dwarkasing, V.N.A.

    2002-01-01

    The cervix uteri is a rigid tube which connects the uterus with the vagina and during most of the time it is firmly closed. During oestrus, late pregnancy and parturition, the morphological appearance in the cervix changes markedly, resulting in a softer cervix, which during parturition can even be

  2. Sexual dysfunction after radiotherapy for cancer of the cervix uteri

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    Ohkawa, R.; Takamizawa, H. (Chiba Univ. (Japan). School of Medicine); Arai, T.; Morita, S.

    1981-03-01

    Investigations of sexual consciousness and sexual dysfunction after radiotherapy for cancer of the cervix uteri were performed on patients of middle and old ages by questionnaires and questioning by doctors, and the following results were obtained. 1. Before radiotherapy, sexual activity was most prominent in their twenties and thirties. However, patients who were in fifties when this study was performed had most active sexual lives during the ages from 35 to 50 years. 2. Frequencies of sexual intercourse decreased markedly just before radiotherapy, and many patients received radiotherapy when sexual activity fell. 3. 32% of the patients have not experienced sexual intercourse after radiotherapy. 4. Decreases in the sex urge, sexuality, vaginal discharge, and frequency of sexual intercourse after radiotherapy were recognized in 77%, 77%, 70%, and 93% respectively. 5. Patients who became unwilling to maintain sexual lives after radiotherapy because of fear about recurrence or aggravation of cancer were 38% by questionaires and 49% by questioning by doctors. 6. Pains on sexual intercourse were found in 69% by questionaires and 49% by questionning by doctors. Most pains occurred at penis insertion and was thought to be due to atrophy and inflammation of vagina and external genitalia in most cases. 7. Both vaginal damage and sexual dysfunction in patients with radiotherapy following surgery for cancer of the cervix uteri, in patients with radiotherapy alone for cancer of the cervix uteri, and in patients with radiotherapy following surgery for cancer of the ovary and corpus uteri were marked, modest and mild, respectively. 8. Vaginal damage score was higher in patients treated more than 5 years before than those less than 2 years ago, but there were no differences in sexual dysfunction score between both groups.

  3. Trends in cancer incidence in female breast, cervix uteri, corpus uteri, and ovary in India.

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    Yeole, Balkrishna B

    2008-01-01

    Trends in breast, cervix uteri, corpus uteri and ovarian cancers in six population based cancer registries (Mumbai, Bangalore, Chennai, Delhi, Bhopal, and Barshi) were evaluated over a period of the last two decades. For studying trends we used a model that fits this data is the logarithm of Y=ABx which represents a Linear Regression model. This approach showed a decreasing trend for cancer of the cervix and increasing trends for cancers of breast, ovary and corpus uteri throughout the entire period of observation in most of the registries. The four cancers, breast, cervix, corpus uteri and ovary, constitute more than 50% of total cancers in women. As all these cancers are increasing, to understand their etiology in depth, analytic epidemiology studies should be planned in a near future on a priority basis.

  4. [Chlamydia trachomatis infection in the cervix uteri].

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    Rosas Arceo, J; Toca Porraz, L; Díaz Esponda, C; Nava Flores, J

    1993-11-01

    We studied 93 gynecological samples of uterine cervix of patients at Hospital de Gineco Obstetricia No. 3, Centro Médico La Raza, IMSS, Health Promotion and Family Medicine Unit No. 5 with monoclonal antibodies. We found Chlamydia trachomatis in 28.4% in women where the infection was suspected. We should think in infection by Chlamydia trachomatis in women with acute cervicitis, acute salpingitis, cervical abnormalities, and to run the specific tests for its detection.

  5. Primary malignant melanoma of cervix and vagina

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    Lee, Jae Hoon; Yun, Jisun; Seo, Jung-Won; Bae, Go-Eun; Lee, Jeong-Won

    2016-01-01

    Primary malignant melanoma (MM) accounts for 1% of all cancers, and only 3% to 7% of these tumors occur in the female genital tract. Data are limited with respect to the basis for treatment recommendations because of the rarity of MM. The overall prognosis of melanomas of the female genital tract is very poor. Two cases of MM of the female genital tract are presented. The first case is of a 70-year-old female patient who complained of left thigh pain and underwent magnetic resonance imaging that showed cervical cancer with involvement of the vagina, bladder, and parametrium, in addition to multiple bony metastases of the proximal femur, acetabulum, and both iliac bones. The second case is of a 35-year-old female patient who suffered from vaginal bleeding for 5 months, and she was diagnosed as having primary vaginal melanoma. The patient underwent radical surgery and two additional surgeries because of recurrence of cancer in both inguinal areas. After surgery, the patient received adjuvant immunotherapy, radiation therapy, and chemotherapy. In both the aforementioned cases, the pathologic diagnosis was made after immunohistochemical analysis, i.e., the tumor cells were stained with HMB-45 and S100, and were found to be positive for both immunostains. PMID:27668208

  6. [Cervix uteri cancer in Poland--epidemiological opening balance and perspectives].

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    Didkowska, Joanna; Wojciechowska, Urszula; Zatoński, Witold

    2006-09-01

    Cancer is one of the main causes of death among young and middle-aged females. In case of some cancer sites there is a possibility of undertaking an intervention, which would diminish the risk of death--to this group belongs first of all malignant neoplasm of cervix uteri. The date of beginning first cervix uteri cancer screening in Poland is approaching, therefore presenting epidemiological opening balance and possible scenarios of changes it worthwhile. This work uses data on morbidity and mortality due to malignant neoplasm of cervix uteri cancer in Polish population. Time trends analysis was based on so-called "breakpoint" (joinpoint analysis). Cervix uteri cancer mortality trend is characterized by two breakpoints (1971 and 1993). In the period of 1963-1970 there was an increase of mortality and then after the trend reversed: percentage decline was estimated at the level of 0.8% yearly in 1971-1992 and 2.4% yearly in 1993-2002. Hypothetical scenarios of changes in cervix uteri cancer mortality show, that lack of intervention will cause mortality among Polish females at the level recorded in Finland 25 years ago. Optimistic variant would allow on diminishing mortality in Poland down to the level observed currently in Finland, in around 30 years. Implementing preventive cervix uteri cancer screening is currently the most urgent challenge of public health. If the preventive screening program will still be in the phase of plans and projects, then in 30 years time Poland will be in the point which Finland reached in the end of 1970s, and our civilizing underdevelopment will reach half a century.

  7. Early response genes in the pathogenesis of cancer of the cervix uteri: a review

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    O. V. Kurmyshkina

    2011-01-01

    Full Text Available Early response genes are a group of proto-oncogenes that are the first to be activated in cell stimulation with different growth factors and to be involved in the regulation of cell proliferation and differentiation. Large amount of information supporting that altered expression of these genes is one of the central and earliest events of carcinogenesis has been accumulated. In this connection, it is promising to use early response genes as diagnostic and prognostic markers for the detection and combination therapy of cancer of the cervix uteri, one of the most common gynecological malignancies characterized by high mortality rates and difficulties in early diagnosis. The theoretical basis for these promises is the found mechanisms for the interaction of early response genes with human papillomavirus genome, the main cause of cervix uteri cancer.

  8. High-dose rate brachytherapy in the treatment of cancer of the cervix uteri

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    D. A. Aliyev

    2011-01-01

    Full Text Available Analysis of the results of examining and treating 246 patients with Stages IIA-IIIB cancer of the cervix uteri (CCU, receiving specific chemoradiotherapy (CRT at the Department of Radiotherapy, National Oncology Center (Baku, has ascertained that CRT using two high-dose (9 Gy rate brachytherapy fractions and competitive cisplatin chemotherapy is an effective, reasonably safe, and economically sound treatment method for locally advanced CCU. The method shows acceptable toxicity and may be used in routine clinical practice.

  9. [DNA in koilocytotic dysplasia of the cervix uteri, cytophotometric studies].

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    Christov, K; Karageosov, I; Makaveeva, V; Kristeva, K

    1987-01-01

    In koilocytotic dysplasia of the uterine cervix the DNA content in squamous cells was quantitated by cytophotometry in histological preparations stained according to Feulgen. Three patterns of DNA distribution in the squamous cells were found. In type one (21.4%) the cells had DNA content in the diploid and paradiploid zone of the histogram. In type two (35.7%) cells with triploid and tetraploid DNA values were found, but with a conspicuous modal class of cells. In type three no modal class cells were found (42.9%). The quantitative DNA changes in squamous cells show that some of the HPV induced alterations may be regarded as precancerous.

  10. RESULTS OF 192IR CONTACT RADIATION THERAPY FOR CERVIX UTERI CANCER

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    O. A. Kravets

    2009-01-01

    Full Text Available The paper presents the results of treatment for locally advanced cervix uteri cancer, by applying a 192Ir radioactive source for contact radiation. Three- and five-year overall and relapse-free survival rates have been obtained for stages: 82.5 and 82.5%; 78.4 and 78.4% for Stage IIb; 57 and 52.3%; 41.6 and 41.6 for IIIb; 53.3 and 47.3%; 42.4 and 37.7% for IVb, respectively.

  11. Adenocarcinoma of the Cervix Uteri and Endometrium Combined With the Kartagener Syndrome on FDG PET/CT.

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    Zhang, Yin; Chen, Yue; Huang, Zhanwen; Zhou, Fan

    2015-11-01

    A 45-year-old woman with pathologically confirmed adenocarcinoma of the cervix uteri and endometrium underwent FDG PET/CT for staging. No metastasis was found. However, the images revealed bronchiectasis, sinusitis, and situs inversus totalis, which are the triad of Kartagener syndrome.

  12. The effect of hyaluronic acid (Cicatridine) on healing and regeneration of the uterine cervix and vagina and vulvar dystrophy therapy.

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    Markowska, J; Madry, R; Markowska, A

    2011-01-01

    Procedures aimed at the treatment of precancerous lesions and ectopia on the uterine cervix are frequently linked to lesions of anatomical structures. The application of hyaluronic acid (Cicatridine vaginal ovules) promotes accelerated healing of the uterine cervix and acquisition of a normal shape in the uterine cervix canal. Local application of hyaluronic acid in the vagina following radiotherapy due to cancer in the uterine cervix or endometrium favourably affects the healing of post-irradiation lesions in the vagina and improves quality of life. Over 90% of patients responded positively to the application of hyaluronic acid in the form of a cream on dystrophic lesions in the vulva. Hyaluronic acid aids the healing process of post-procedural wounds in the uterine cervix, following radiotherapy applied due to cancer of the uterine cervix, endometrium and in vulvar dystrophy.

  13. [Life threatening postpartal haemorrhage after rupture of the vagina, uterine cervix, caesarean section or hysterectomy].

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    Kozovski, I; Radoinova, D

    2010-01-01

    The authors discuss 10 cases--seven after vaginal and cervical rupture, 2 after Caesarean section and 1 after hysterectomy. Six of them died--5 after rupture of the vagina and cervix and one after Caesarean section. The lethal issue was avoidable in all cases because it was a result of untimely done or not done at all hysterectomy and other interventions, e.g., ligation of the hypogastric arteries, as well as of faulty surgical performance. Basic principles of surgical behavior in such cases are postulated.

  14. Establishment and characterization of a differentiated epithelial cell culture model derived from the porcine cervix uteri

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    Miessen Katrin

    2012-03-01

    Full Text Available Abstract Background Cervical uterine epithelial cells maintain a physiological and pathogen-free milieu in the female mammalian reproductive tract and are involved in sperm-epithelium interaction. Easily accessible, differentiated model systems of the cervical epithelium are not yet available to elucidate the underlying molecular mechanisms within these highly specialized cells. Therefore, the aim of the study was to establish a cell culture of the porcine cervical epithelium representing in vivo-like properties of the tissue. Results We tested different isolation methods and culture conditions and validated purity of the cultured cells by immunohistochemistry against keratins. We could reproducibly culture pure epithelial cells from cervical tissue explants. Based on a morphology score and the WST-1 Proliferation Assay, we optimized the growth medium composition. Primary porcine cervical cells performed best in conditioned Ham's F-12, containing 10% FCS, EGF and insulin. After cultivation in an air-liquid interface for three weeks, the cells showed a discontinuously multilayered phenotype. Finally, differentiation was validated via immunohistochemistry against beta catenin. Mucopolysaccharide production could be shown via alcian blue staining. Conclusions We provide the first suitable protocol to establish a differentiated porcine epithelial model of the cervix uteri, based on easily accessible cells using slaughterhouse material.

  15. Reliability of recording uterine cancer in death certification in France and age-specific proportions of deaths from cervix and corpus uteri.

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    Rogel, Agnès; Belot, Aurélien; Suzan, Florence; Bossard, Nadine; Boussac, Marjorie; Arveux, Patrick; Buémi, Antoine; Colonna, Marc; Danzon, Arlette; Ganry, Olivier; Guizard, Anne-Valérie; Grosclaude, Pascale; Velten, Michel; Jougla, Eric; Iwaz, Jean; Estève, Jacques; Chérié-Challine, Laurence; Remontet, Laurent

    2011-06-01

    French uterine cancer recordings in death certificates include 60% of "uterine cancer, Not Otherwise Specified (NOS)"; this hampers the estimation of mortalities from cervix and corpus uteri cancers. The aims of this work were to study the reliability of uterine cancer recordings in death certificates using a case matching with cancer registries and estimate age-specific proportions of deaths from cervix and corpus uteri cancers among all uterine cancer deaths by a statistical approach that uses incidence and survival data. Deaths from uterine cancer between 1989 and 2001 were extracted from the French National database of causes of death and case-to-case matched to women diagnosed with uterine cancer between 1989 and 1997 in 8 cancer registries. Registry data were considered as "gold-standard". Among the 1825 matched deaths, cancer registries recorded 830 cervix and 995 corpus uteri cancers. In death certificates, 5% and 40% of "true" cervix cancers were respectively coded "corpus" and "uterus, NOS" and 5% and 59% of "true" corpus cancers respectively coded "cervix" and "uterus, NOS". Miscoding cervix cancers was more frequent at advanced ages at death and in deaths at home or in small urban areas. Miscoding corpus cancers was more frequent in deaths at home or in small urban areas. From the statistical method, the estimated proportion of deaths from cervix cancer among all uterine cancer deaths was higher than 95% in women aged 30-40 years old but declined to 35% in women older than 70 years. The study clarifies the reason for poor encoding of uterus cancer mortality and refines the estimation of mortalities from cervix and corpus uteri cancers allowing future studies on the efficacy of cervical cancer screening.

  16. In vivo Raman spectroscopy of human uterine cervix: exploring the utility of vagina as an internal control

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    Shaikh, Rubina; Dora, Tapas Kumar; Chopra, Supriya; Maheshwari, Amita; Kedar K., Deodhar; Bharat, Rekhi; Krishna, C. Murali

    2014-08-01

    In vivo Raman spectroscopy is being projected as a new, noninvasive method for cervical cancer diagnosis. In most of the reported studies, normal areas in the cancerous cervix were used as control. However, in the Indian subcontinent, the majority of cervical cancers are detected at advanced stages, leaving no normal sites for acquiring control spectra. Moreover, vagina and ectocervix are reported to have similar biochemical composition. Thus, in the present study, we have evaluated the feasibility of classifying normal and cancerous conditions in the Indian population and we have also explored the utility of the vagina as an internal control. A total of 228 normal and 181 tumor in vivo Raman spectra were acquired from 93 subjects under clinical supervision. The spectral features in normal conditions suggest the presence of collagen, while DNA and noncollagenous proteins were abundant in tumors. Principal-component linear discriminant analysis (PC-LDA) yielded 97% classification efficiency between normal and tumor groups. An analysis of a normal cervix and vaginal controls of cancerous and noncancerous subjects suggests similar spectral features between these groups. PC-LDA of tumor, normal cervix, and vaginal controls further support the utility of the vagina as an internal control. Overall, findings of the study corroborate with earlier studies and facilitate objective, noninvasive, and rapid Raman spectroscopic-based screening/diagnosis of cervical cancers.

  17. [Ultrastructural organization of the epithelial layers and surface morphological characteristics of cells in adenocarcinomas of the cervix uteri].

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    Chernyĭ, A P

    1984-08-01

    The cell interrelations, and cellular attachment to the stroma in normal columnar epithelium and adenocarcinoma of the cervix uteri were examined by transmission and scanning electron microscopy. The application of rapid enzymatic digestion technique allows to visualize the topography of cell membranes, otherwise disguised in ordinary conditions. Four types of disordered epithelial sheets characterized by different apical, lateral and basal cell surface changes are described. Various alterations in morphology of the basement membrane and adjacent conjunctive tissue are associated with the tumor appearance. Marked deviations in cell-stroma contact may lead to the inversion of cell polarity revealed in cervical adenocarcinoma: cellular parts adjoining to stroma acquire characteristic features of the apical pole.

  18. Radionuclide diagnosis of ureteric function in patients with stages I-IV cancer of the cervix uteri

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    L. A. Ashrafyan

    2010-01-01

    Full Text Available The experience with serial renal scintigraphy demonstrated its high informative value and safety in evaluating the severity of intrarenal urine outflow disorders; however, failure to make an objective assessment of ureteral patency considerably limits its study. The set of studies, which is given in this paper, is devoted to precisely this, highly urgent, problem. The authors describe an original procedure for diagnosing impaired urine outflow along the ureters, which has been used during serial renal scintigraphy, define the visual and digital characteristics of normal and impaired urine outflow in the supravesical segment, and denote the criteria characterizing severe impair- ments of renal urine derivation along the ureters. Risk factors for urine outflow disorders have been identified in patients with cancer of the cervix uteri, who receive various treatment modalities.

  19. [Comparative estimation of results of remote and combined radiotherapy in patients with cancer of the cervix uteri of the III-IV stages of disease].

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    Pereslegin, I A; Makarov, O V; Semko, V F; Frolova, E L

    2000-01-01

    The paper presents a procedure of teleradiotherapy in patients with stages III-IV cancer of the cervix uteri with significant concurrent pathology. Control patients with the similar disease stages underwent combined radiation therapy. If there are contraindications to combined radiation therapy, teleradiotherapy is possible and required as an independent treatment that prolongs and improves the patients' like quality irrespective of the extent of a tumorous process.

  20. [Carcinoma of the cervix uteri--the morphological changes after preoperative radiotherapy].

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    Veselinova, T; Ivanova, R; Gorchev, G; Tsvetkov, Ch

    1998-01-01

    A retrospective morphological research is made on 11 patients with squamous cell carcinoma of the cervix for a three-years period of time (1995-1997). Preoperative radiotherapy (intracavitary radiotherapy with Cs and/or external radiation) is carried out. A control group of 10 patients with cancer of uterine cervix without preoperative radiotherapy is also studied. All of the women undergo a wider hysterectomy by the method of Piver class IV. The radiation alterations in the tumor and the surrounding normal uterine tissues are also researched. A full regression of the tumor Ts size. There are demonstrative changes in the tumor and surrounding normal tissues, which are the prove the answer to the ionizing irradiation.

  1. [Concomitant radiochemotherapy in cancer of the cervix uteri: modifications of the standards].

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    Haie-Meder, C; Lhommé, C; de Crevoisier, R; Morice, P; Resbeut, M

    2000-11-01

    For a long time, combined external irradiation and brachytherapy has been considered as the standard treatment in patients with advanced cervical cancers. Recently, five clinical randomized trials assessing the role of cis-platin-based chemotherapy delivered concomitantly to radiotherapy have been conducted in the United States. Another clinical randomized trial assessing the role of epirubicin was conducted by the Queen Mary Hospital in Hong Kong. With more than 2,000 enrolled patients, these studies showed converging results with an overall and disease-free survival improvement in the arms combining cis-platin and epirubicin-based chemotherapy and irradiation. These combinations led to a significant decrease in loco-regional evolution or recurrence rates, or even in pulmonary metastases rates. The relative risk of recurrences was decreased by 50%. The relative risk of death was decreased by 40%. The differences, however, were less significant in patients with advanced stages III or IVA. Hematological and digestive acute toxicity was significantly higher in the radiochemotherapy groups, but long-term complications were comparable. The results of these randomized trials have led to a modification in the standard of treatment in these poor prognosis cervix cancers. Five of the randomized trials evidenced the superiority of cis-platin-based chemotherapy, but the optimal chemotherapeutic regimens remain to be defined.

  2. Influence of pregnancy and labor on the occurrence of nerve fibers expressing the capsaicin receptor TRPV1 in human corpus and cervix uteri

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    Irestedt Lars

    2008-02-01

    Full Text Available Abstract Background Cervical ripening is a prerequisite for a normal obstetrical outcome. This process, including labor, is a painful event that shares features with inflammatory reactions where peripheral nociceptive pathways are involved. The capsaicin and heat receptor TRPV1 is a key molecule in sensory nerves involved in peripheral nociception, but little is known regarding its role in the pregnant uterus. Therefore, the aim of this study was to investigate human corpus and cervix uteri during pregnancy and labor and non-pregnant controls for the presence of TRPV1. Methods We have investigated human uterine corpus and cervix biopsies at term pregnancy and parturition. Biopsies were taken from the upper edge of the hysterotomy during caesarean section at term (n = 8, in labor (n = 8 and from the corresponding area in the non-pregnant uterus after hysterectomy (n = 8. Cervical biopsies were obtained transvaginally from the anterior cervical lip. Serial frozen sections were examined immunohistochemically using specific antibodies to TRPV1 and nerve markers (neurofilaments/peripherin. Results In cervix uteri, TRPV1-immunoreactive fibers were scattered throughout the stroma and around blood vessels, and appeared more frequent in the sub-epithelium. Counts of TRPV1-immunoreactive nerve fibers were not significantly different between the three groups. In contrast, few TRPV1-immunoreactive fibers were found in nerve fascicles in the non-pregnant corpus, and none in the pregnant corpus. Conclusion In this study, TRPV1 innervation in human uterus during pregnancy and labor is shown for the first time. During pregnancy and labor there was an almost complete disappearance of TRPV1 positive nerve fibers in the corpus. However, cervical innervation remained throughout pregnancy and labor. The difference in TRPV1 innervation between the corpus and the cervix is thus very marked. Our data suggest that TRPV1 may be involved in pain mechanisms associated with

  3. The Radiotherapy Effect of 512 Cases of Carcinoma of Cervix Uteri%512例子宫颈癌放射治疗疗效报告

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    LI Ailing; 李爱苓; SUN Jianheng; 孙建衡; ZHANG Wenhua; 张蓉

    2004-01-01

    Objective: To report treatment results of 512 cases of carcinoma of uteri and investigate the way to improve treatment effects. Methods: The 5-year survival rate and the factors influencing the prognosis of 512 cases of uteri were analyzed retrospectively. Results: The 5-year survival rate after radiotherapy was as follows: stage Ⅰ: 100%; stage Ⅱ: 74.5%; stage Ⅲ: 56.5%; stage Ⅳ: 28.6%. Overall 5-year survival rate was 65.4%, which was almost the same as traditional intracavitary injection (65.7%). The 5-year survival rate in cervix lesions more than 4 cm and those less than or equal to 4 cm in stage Ⅱ was 63.9% and 79.3%,respectively, with statistically significant difference (P<0.05). The 5-year survival rate of the patients with squamous carcinoma in stage Ⅱ was 77.0% and that with low differentiated squamous carcinoma was 64.0%respectively. Twenty-three (4.5%) out of 512 suffered from post-radiotherapy rectitis, 18 (3.5%) cystitis.Conclusion: the survival rate of the patients with cervix carcinoma subject to radiotherapy is stabilized to 65%. The diameter and pathological classes affect survival rate, respectively. Low radiotherapy dose decrease complications.

  4. Dosimetric evaluation of a combination of brachytherapy applicators for uterine cervix cancer with involvement of the distal vagina; Avaliacao dosimetrica de uma combinacao de aplicadores para braquiterapia de tumores do colo uterino com acometimento da porcao distal da vagina

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    Guimaraes, Roger Guilherme Rodrigues [Real e Benemerita Sociedade Portuguesa de Beneficencia, Sao Paulo, SP (Brazil). Servico de Radioterapia Estereotactica; Carvalho, Heloisa de Andrade; Stuart, Silvia Radwanski; Rubo, Rodrigo Augusto [Universidade de Sao Paulo (USP), SP (Brazil). Hospital das Clinicas. Servico de Radioterapia], e-mail: handrade@hcnet.usp.br; Seraide, Rodrigo Migotto [Centro de Oncologia Campinas, SP (Brazil)

    2009-07-15

    Objective: To evaluate an alternative brachytherapy technique for uterine cervix cancer involving the distal vagina, without increasing the risk of toxicity. Materials And Methods: Theoretical study comparing three different high-dose rate intracavitary brachytherapy applicators: intrauterine tandem and vaginal cylinder (TC); tandem/ring applicator combined with vaginal cylinder (TR+C); and a virtual applicator combining both the tandem/ring and vaginal cylinder in a single device (TRC). Prescribed doses were 7 Gy at point A, and 5 Gy on the surface or at a 5 mm depth of the vaginal mucosa. Doses delivered to the rectum, bladder and sigmoid colon were kept below the tolerance limits. Volumes covered by the isodoses, respectively, 50% (V50), 100% (V100), 150% (V150) and 200% (V200) were compared. Results: Both the combined TR+C and TRC presented a better dose distribution as compared with the TC applicator. The TR+C dose distribution was similar to the TRC dose, with V150 and V200 being about 50% higher for TR+C (within the cylinder). Conclusion: Combined TR+C in a two-time single application may represent an alternative therapy technique for patients affected by uterine cervix cancer involving the distal vagina. (author)

  5. [Maturation of the cervix uteri using prostaglandin F2 alpha before induction of labor in pathologic pregnancies].

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    Maria, B; Fayette, E; Stampf, F; Gandon, C; Gantrel, J; Barrat, J

    1983-01-01

    It is possible to induce labour in pathological pregnancies after artificial ripening of the cervix. The present study concerns 70 patients (45 primipara, 25 multipara). The main pathologies are hypertension of pregnancy and pregnancies past dates. Prostaglandin F2 alpha has been used with a Tylose gel containing 5 mg of PGF2 alpha introduced by the extra-amniotic route. The cervical change was noted using Bishop's score. The mean increase of the cervical score was 0.8 with the first PGF2 alpha gel. The total mean increase was 1.2. Two cases of hyperstimulation of the uterus were observed and they led to Caesarean section. Prostaglandin gel induced labour in 56% of the patients. The mean time between the introduction of the gel and the delivery was 14 h for primipara and 10 h for multipara. Other patients were induced with oxytocin on the following day. Epidural analgesia was widely used in this study (in 64% of cases). The mean duration of labour was 6 h 10 for primipara and 4 h 30 for multipara. 30% of the patients needed Caesarean section but there was a marked difference between primipara (36%) and multipara (4%). After a review of the literature the authors conclude that it is useful to ripen the cervix prostaglandin but, as foreign authors do, they think that PGE2 should be more efficient.

  6. Comparison of a 3-D multi-group SN particle transport code with Monte Carlo for intracavitary brachytherapy of the cervix uteri.

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    Gifford, Kent A; Wareing, Todd A; Failla, Gregory; Horton, John L; Eifel, Patricia J; Mourtada, Firas

    2009-12-03

    A patient dose distribution was calculated by a 3D multi-group S N particle transport code for intracavitary brachytherapy of the cervix uteri and compared to previously published Monte Carlo results. A Cs-137 LDR intracavitary brachytherapy CT data set was chosen from our clinical database. MCNPX version 2.5.c, was used to calculate the dose distribution. A 3D multi-group S N particle transport code, Attila version 6.1.1 was used to simulate the same patient. Each patient applicator was built in SolidWorks, a mechanical design package, and then assembled with a coordinate transformation and rotation for the patient. The SolidWorks exported applicator geometry was imported into Attila for calculation. Dose matrices were overlaid on the patient CT data set. Dose volume histograms and point doses were compared. The MCNPX calculation required 14.8 hours, whereas the Attila calculation required 22.2 minutes on a 1.8 GHz AMD Opteron CPU. Agreement between Attila and MCNPX dose calculations at the ICRU 38 points was within +/- 3%. Calculated doses to the 2 cc and 5 cc volumes of highest dose differed by not more than +/- 1.1% between the two codes. Dose and DVH overlays agreed well qualitatively. Attila can calculate dose accurately and efficiently for this Cs-137 CT-based patient geometry. Our data showed that a three-group cross-section set is adequate for Cs-137 computations. Future work is aimed at implementing an optimized version of Attila for radiotherapy calculations.

  7. HT在宫颈癌术后盆腔放疗的优势探讨%Treatment and dosimetry advantage between FF-IMRT, VMAT, and HT in cervix uteri radiotherapy

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    杨波; 庞廷田; 刘峡; 刘楠; 胡克; 邱杰; 张福泉

    2014-01-01

    Objective To investigate dosimetric advantage of fixed field intensity-modulated radiotherapy (FF-IMRT),volumetric modulated arc therapy (VMAT) and helical tomotherapy (HT) for cervix uteri cancer.Methods CT datasets of ten patients with cervix uteri cancer were enrolled in the study.FF-IMRT,VMAT and HT plans were designed on Eclipse and HT treatment planning system.Plans were optimized with the aim to assess OAR while enforcing highly conformal target coverage.Institutional dose-volume constraints used in cervix uteri cancer were kept the same for three techniques.The different of three plan was play by single factor analysis of variance and compared to two groups by LSD method.Results All FF-IMRT,VMAT and HT resulted in equivalent target coverage but HT had an improved homogeneity index (P =0.000) and conformity index (P =0.000),or PTV of 105% prescription dose (47.12%,45.83% and 0.05%,P=0.000) and lowest Dmax dose (54.53 Gy,53.65 Gy,52.69 Gy,P=0.000).Compared with FF-IMRT and VMAT,the bladder V40 and Dmax of HT were lowest (50.01%,46.84%,42.98%,P=0.001 and 54.49 Gy,52.96 Gy,52.78 Gy,P=0.000),with the rectum V40 lowest (54.61%,48.34%,46.78%,P =0.006),the intestine Dmax lowest (54.53 Gy,53.65 Gy,52.66 Gy,P =0.000) and marrow Dmax lowest (54.51 Gy,54.44 Gy,52.13 Gy,P=0.000).But the delivery MU per fraction were highest (1429.20 MU,617.80 MU,7002.04 MU,P =0.000).Conclusions HT technology is feasible for clinical applications in cervical uteri cancer and can be used as a new method to promote.%目的 比较宫颈癌术后患者应用HT、固定野IMRT、VMAT的剂量学差异.方法 选择10例宫颈癌术后放疗患者的CT图像进行靶区及OAR勾画,并对同一CT图像设计固定野IMRT、VMAT、HT计划.评估靶区及OAR的剂量分布.采用单因素方差分析3个计划参数差异,用LSD法进行两两比较.结果 IMRT、VMAT、HT计划的靶区覆盖度均满足临床处方剂量要求,但HT计划靶区覆盖度最优(P=0.000),

  8. 外阴、阴道和宫颈尖锐湿疣组织中HPV感染的对比研究%A comparative study of HPV infection in condyloma acuminate tissues of vulva,vagina and cervix

    Institute of Scientific and Technical Information of China (English)

    单慧敏; 施启丰; 耿建祥; 仲玉英; 梅静; 龙秀荣; 夏林; 王宏景; 赵雪

    2016-01-01

    目的:研讨外阴、阴道和宫颈尖锐湿疣(CA)组织中人乳头瘤病毒(HPV)感染型别的分布状况以及临床意义。方法应用基因芯片结合PCR技术对63例外阴尖锐湿疣、61例阴道尖锐湿疣和65例宫颈尖锐湿疣组织行23种HPV基因型检测,并分析患者的临床病理资料。结果63例外阴尖锐湿疣组织中检出HPV阳性者56例,HPV感染率为88.89%(56/63);61例阴道尖锐湿疣组织中检出HPV阳性者55例,HPV感染率为90.16%(55/61);65例宫颈尖锐湿疣组织中检出HPV阳性者62例,HPV感染率为95.39%(62/65)。结论HPV感染与外阴、阴道和宫颈尖锐湿疣的发病密切相关,HPV6和11是主流型别,以外阴尖锐湿疣最为常见。基因芯片结合PCR技术是适合应用于临床行HPV分型诊断的一种方法,具有敏感性好、特异性高的特点。对女性外阴、阴道和宫颈尖锐湿疣的临床诊断、治疗及其疫苗的研究具有重要的意义。%Objective To study the distribution status and clinical significance of human papillomavirus(HPV) infection geno‐types in condyloma acuminate(CA) tissues of vulva ,vagina and cervix .Methods The gene‐chips combined with polymerase chain reaction (PCR) technology were utilized for detecting 23 kinds of HPV genotypes in tissue specimens from 63 cases of vulval CA , 61 cases of vaginal CA and 65 cases of cervical CA .Their clinical pathological data were analyzed .Results In 63 cases of vulval CA ,56 cases were HPV positive with the HPV infection rate of 88 .89% (56/63) ,in 61 cases of vaginal CA ,55 cases were HPV positive with the HPV infection rate of 90 .16% (55/61) ,and in 65 cases of cervical CA ,62 cases were HPV positive with the HPV infection rate of 95 .39% (62/65) .Conclusion HPV infection is closely related to the CA pathgenesis in vulva ,vagina and cervix . HPV6 and HPV 11 are main stream genotypes ,in which vulval CA is most common .The gene

  9. Avaliação dosimétrica de uma combinação de aplicadores para braquiterapia de tumores do colo uterino com acometimento da porção distal da vagina Dosimetric evaluation of a combination of brachytherapy applicators for uterine cervix cancer with involvement of the distal vagina

    Directory of Open Access Journals (Sweden)

    Roger Guilherme Rodrigues Guimarães

    2009-08-01

    Full Text Available OBJETIVO: Avaliar uma alternativa de braquiterapia para tumores do colo uterino acometendo a porção distal da vagina, sem aumentar os riscos de toxicidade. MATERIAIS E MÉTODOS: Estudo teórico comparando três diferentes aplicadores de braquiterapia intracavitária de alta taxa de dose: sonda intrauterina e cilindro vaginal (SC; sonda e anel associado ao cilindro vaginal (SA+C e um aplicador virtual com sonda, anel e cilindro vaginal em um único conjunto (SAC. Foram prescritas doses de 7 Gy no ponto A e 5 Gy na superfície ou a 5 mm de profundidade na mucosa vaginal, mantendo as doses nos pontos de reto, bexiga e sigmoide abaixo dos limites de tolerância. Foram comparados os volumes englobados pelas isodoses de 50% (V50, 100% (V100, 150% (V150 e 200% (V200, respectivamente. RESULTADOS: Tanto SA+C quanto SAC apresentaram melhor distribuição de dose quando comparados ao aplicador SC. A distribuição de dose obtida com SA+C foi semelhante à do aplicador SAC, sendo V150 e V200 cerca de 50% maiores para SA+C, todavia, dentro do cilindro. CONCLUSÃO: A utilização de SA+C em uma única aplicação em dois tempos pode ser uma alternativa de tratamento para pacientes que apresentam tumores de colo uterino com acometimento distal da vagina.OBJECTIVE: To evaluate an alternative brachytherapy technique for uterine cervix cancer involving the distal vagina, without increasing the risk of toxicity. MATERIALS AND METHODS: Theoretical study comparing three different high-dose rate intracavitary brachytherapy applicators: intrauterine tandem and vaginal cylinder (TC; tandem/ring applicator combined with vaginal cylinder (TR+C; and a virtual applicator combining both the tandem/ring and vaginal cylinder in a single device (TRC. Prescribed doses were 7 Gy at point A, and 5 Gy on the surface or at a 5 mm depth of the vaginal mucosa. Doses delivered to the rectum, bladder and sigmoid colon were kept below the tolerance limits. Volumes covered by the

  10. 高危型人乳头瘤病毒感染与宫颈癌前病变及宫颈癌的相关性分析%Correlation analysis between high-risk human papillomavirus infection and precancerous lesions of cervix uteri and cervical cancer

    Institute of Scientific and Technical Information of China (English)

    李曼红; 曾庆纯

    2015-01-01

    Objective To investigate the correlation between high-risk human papillomavirus (HPV) infection, precancerous lesions of cervix uteri and cervical cancer. Methods Among 120 patients with cervical cancer or precancerous lesions, there were 60 cases with precancerous lesions of cervix uteri (precancerous lesions group) and 60 cases with cervical cancer (cervical cancer group). They were chosen as the observation group, and another 60 patients without cytological changes of cervix uteri in colpitis were taken as the control group. Cervix uteri thinprep cytology test, cervical scraping smear, and immunohistochemcial method were applied for screening and analysis, and Ann PCR-reverse dot blot method was used for detection. Examination results were compared between the groups. Results The total positive rate of high-risk HPV was 56.67%, and the positive rates of high-risk HPV in precancerous lesions group, cervical cancer group and control group were respectively 65%, 95%, and 10%. The difference of positive rate between the three groups had statistical significance (P<0.05). Conclusion High-risk HPV has close relationship with precancerous lesions of cervix uteri and cervical cancer, and the infection of high-risk HPV was mainly in HPV16.%目的:探讨高危型人乳头瘤病毒(HPV)感染与宫颈癌前病变以及宫颈癌的相关性。方法120例宫颈癌或是癌前病变患者,其中60例为宫颈癌前病变患者(癌前病变组),60例为宫颈癌患者(宫颈癌组),将其归为观察组,同时期60例阴道炎宫颈无细胞学改变的患者设为对照组。采用宫颈液基细胞学检查法与宫颈刮片、免疫组化予以筛查分析,同时还采用达安PCR-反向点杂交法检测,对比分析各组检测结果。结果高危型HPV总阳性率为56.67%,癌前病变组、宫颈癌组以及对照组患者的高危型HPV阳性率分别为65%、95%、10%,三组患者的阳性率对比差异具有统计学意义(P<0.05)。结论高

  11. Human Papillomavirus Infection of Cervix Uteri in HIV-positive Women from Hengyang★%衡阳地区HIV阳性妇女宫颈HPV感染情况研究★

    Institute of Scientific and Technical Information of China (English)

    谢小平; 唐双阳; 刘安元; 李乐

    2013-01-01

      Objective To study the infection of Human papilloma virus (HPV) among HIV-positive women from Hengyang, and to provide a scientific basis for the HPV epidemiological research on HIV-positive groups. Methods A total of 4576 women were tested for sero-antibody of HIV by ELISA and immuno-colloidal golden method. The positive speci-mens detected by both methods were further sent out to confirm. Meanwhile 4576 cervical tissue specimens were examined for HPV by PCR and membrane hybridization method. Results The rate of HIV infection was 0.83% in 4 576 women, and that of HPV was 21.02%. The HPV-positive rate of 60.53% in HIV-positive group had statistical difference from that of 20.69% in HIV-negative group (χ2=36.02, P<0.005). Among 962 HPV-positive women, the rate of mixed HPV infection in HIV-positive group was 56.52%, but that in HIV-negative group was 20.98%; there were statistical difference between the two groups (χ2=16.62, P<0.005). Conclusion The rate of HPV infection of cervix Uteri in HIV-positive women was high, and high risk HPV and multiple HPV infection happened commonly, both of which indicated that HPV infection had close relation with HIV-positive patients.%  目的了解本地区人类免疫缺陷病毒(HIV)阳性女性宫颈人乳头瘤病毒(HPV)感染状况,为HIV阳性人群中HPV感染的防治提供依据。方法采用ELISA法和胶体金法检测4576例女性患者血清或血浆中的HIV抗体,两种方法均为阳性标本再送检确认。PCR+膜杂交法检测病人宫颈脱落细胞及宫颈粘液标本进行HPV病毒基因分型。结果4576例病例中,HIV感染率为0.83%;HPV感染率为21.02%。HIV阳性组中HPV感染率为60.53%,HIV阴性组中HPV感染率20.69%,差异有统计学意义(χ2=36.02,P<0.005)。在962例HPV阳性病例中,HIV阳性组的混合HPV感染率为56.52%;HIV阴性组的混合HPV感染率为20.98%;差异有统计学意义(χ2=16.62,P<0.005)。结论HIV阳性妇

  12. A case of small cell carcinoma of the vagina

    Directory of Open Access Journals (Sweden)

    Ryosuke Tamura

    2013-12-01

    Full Text Available Primary small cell carcinoma of the vagina is quite rare, and a standard treatment has not been established yet. Herein, we report a case of an 81-year-old woman who was diagnosed with a vaginal tumor without continuity with the uterine cervix. Histopathological diagnosis indicated alveolar solid growth of nuclear chromatin-rich atypical cells with a high N/C ratio and a partially recognized rosette-like structure, suggesting a differentiated neuroendocrine system. Chromogranin A and synapto- physin were positive. Stage I vaginal small cell carcinoma localized to the vagina was diagnosed. The tumor disappeared by radiation monotherapy with external beam irradiation and endocavitary irradiation. The patient remains alive without any disease 1 year and 8 months after the treatment, suggesting the efficacy of radiotherapy in small cell carcinoma of the vagina.

  13. Surgical treatment of a huge submucous myoma prolapsed into the vagina

    OpenAIRE

    AKDAŞ REİS, Yıldız; AKDAĞ CIRIK, Derya; TAŞCI, Tolga; SALİHOĞLU, Kerime; Boran, Nurettin; TULUNAY, Gokhan; Turan, Taner

    2015-01-01

    Nearly 5% of all myomas are originated from the smooth muscle of uterus, beneath the endometrium and called as ‘submucous myoma’. Submucous myomas distort the cavity when they enlarge and may even pass the cervix and reach to the vagina by uterine contractions. The most common treatment of the myomas prolapsing the vagina is the vaginal myomectomy. However, abdominal treatment may be necessary when the submucous myom grow within the cavity and enlarge the uterus. This paper presents a case of...

  14. Insufficient cervix

    Science.gov (United States)

    ... by the 4th month Having past late-term abortions Having a cervix that did not develop normally ... of Gynecology and Obstetrics at Johns Hopkins University School of Medicine, Baltimore, MD. Review provided by VeriMed ...

  15. A comunicação na abordagem preventiva do câncer do colo do útero: importância das influências histórico-culturais e da sexualidade feminina na adesão às campanhas Communication in the prevention of cervix uteri cancer: the importance of historical cultural influences and of feminine sexuality in the adhesion to campaigns

    Directory of Open Access Journals (Sweden)

    Luciana Maria Britto da Cruz

    2008-06-01

    Full Text Available Considerando que o câncer cérvico-uterino ainda se apresenta como problema de saúde pública no Brasil e que os programas governamentais de prevenção existentes para esse câncer não alcançaram a meta estimada, visto que a cobertura e o número de atendimentos nas campanhas não atingiram um quarto do estimado na primeira campanha; e, na segunda, o alcance foi inferior à primeira, este artigo se propôs a levantar questões referentes a não adesão de muitas mulheres às campanhas. Para isso, foram ressaltadas a relevância epidemiológica para a abordagem do tema; a importância do corpo para a mulher; e as influências histórico-culturais no comportamento preventivo das mulheres. Dessa forma, foi realizado um estudo teórico descritivo, usando como fonte principal de informações dados do Ministério da Saúde e do Instituto Nacional do Câncer (INCA e como fonte de apoio livros, artigos científicos, periódicos e dissertações que abordam a temática proposta. A fim de discutir possibilidades estratégicas que possam ser eficazes para uma maior adesão feminina às campanhas preventivas, foi considerada a importância da forma de abordagem dos profissionais de saúde, salientando para que atuem correspondendo aos valores culturais apresentados pelas mulheres. Além disso, ressaltou-se a linguagem utilizada nas campanhas de prevenção do câncer do colo do útero, atentando para o fato de que conceitos transmitidos podem endossar valores culturais de abordagem do feminino que dificultam a identificação e a adesão de muitas mulheres.Cervix uteri cancer is still a public health problem in Brazil, but the governmental prevention programs that exist for this type of cancer have not reached the estimated index of covering. The number of people assisted in the campaigns has not reached one quarter of what was estimated in the first campaign and in the second one the number was even inferior. In light of this situation, this article

  16. Male form of persistent Mullerian duct syndrome type I (hernia uteri inguinalis presenting as an obstructed inguinal hernia: a case report

    Directory of Open Access Journals (Sweden)

    Gujar Nishikant N

    2011-12-01

    Full Text Available Abstract Introduction Persistent Mullerian duct syndrome is a rare form of male pseudo-hermaphroditism characterized by the presence of Mullerian duct structures in an otherwise phenotypically, as well as genotypically, normal man; only a few cases have been reported in the worldwide literature. We report the case of a 30-year-old man with unilateral cryptorchidism on the right side and a left-sided obstructed inguinal hernia containing a uterus and fallopian tube (that is, hernia uteri inguinalis; type I male form of persistent Mullerian duct syndrome coincidentally detected during an operation for an obstructed left inguinal hernia. Case presentation A 30-year-old South Indian man was admitted to our facility with a left-sided obstructed inguinal hernia of one day's duration. He had a 12-year history of inguinal swelling and an absence of the right testis since birth. Our patient had well developed masculine features. Local physical examination revealed a left-sided obstructed inguinal hernia with an absence of the right testis in the scrotum. Exploration of the inguinal canal revealed an indirect inguinal hernia containing omentum, the left corner of the uterus and a left fallopian tube. Extension of the incision revealed a well formed uterus, cervix and upper part of the vagina attached to the prostate by a thick fibrosed band. Total excision of the uterus, bilateral fallopian tubes and right testis was performed. A biopsy was taken from the left testis. The operation was completed by left inguinal herniorraphy. Histopathological examination of the hernial contents was consistent with that of a uterus and fallopian tubes without ovaries. Both testes were atrophied, with complete arrest of spermatogenesis. Post-operative karyotype analyses were negative for 46,XY and Barr bodies on buccal smear. A semen examination revealed azoospermia with a low serum testosterone level. Conclusions In cases of unilateral or bilateral cryptorchidism

  17. [Cervix cancer].

    Science.gov (United States)

    Pointreau, Y; Ruffier Loubière, A; Denis, F; Barillot, I

    2010-11-01

    Cervix cancers declined in most developed countries in recent years, but remain, the third worldwide leading cause of cancer death in women. A precise staging, based on clinical exam, an abdominal and pelvic MRI, a possible PET-CT and a possible lymph node sampling is necessary to adapt the best therapeutic strategy. In France, the treatments of tumors of less than 4 cm without nodal involvement are often based on radiotherapy followed by surgery and, whereas tumors larger than 4 cm and involved nodes are treated with concurrent chemoradiotherapy. Based on an illustrated clinical case, indications, delineation, dosimetry and complications expected with radiotherapy are demonstrated.

  18. Pain management in cancer cervix

    Directory of Open Access Journals (Sweden)

    Palat Gayatri

    2005-01-01

    Full Text Available Cancer of the cervix uteri is a common cause of pain among women. On the physical realm, the cancer may cause somatic [soft tissue and bone], visceral and neuropathic pain [lumbosacral plexopathy]. Radiotherapy and chemotherapy may cause neuropathy too. Psychological, social and cultural factors modify the pain. Evaluation of the individual type of pain and a patient-centred approach are fundamental requirements for rational management. Disease modifying treatment like radiotherapy and chemotherapy must be considered when applicable. Pain control is usually achieved by the use of WHO three-step ladder, remembering that possible association of renal dysfunction would necessitate caution in the use of NSAIDs and opioids. Side effects must be anticipated, prevented when possible, and aggressively treated; nausea and vomiting may already be present, and constipation can worsen pain when there is a pelvic mass. Pain emergencies can be treated by quick titration with intravenous morphine bolus doses. Neuropathic pain may warrant the use of usual adjuvants, with particular reference to cortico-steroids and the NMDA antagonist, ketamine. In intractable pain, many neurolytic procedures are tried, but a solid evidence base to justify their use is lacking. Continuous epidural analgesia with local anaesthetic and opioid may be needed when drug therapy fails, and desperate situations may warrant interventions such as neurolysis. Such physical measures for pain relief must be combined with psychosocial support and adequate explanations to the patient and the family.

  19. Acuidade da citologia oncótica para o diagnóstico da infecção pelo HPV no colo uterino de mulheres portadoras do HIV Accuracy of oncotic cytology for HPV infection diagnosis on the cervix uteri of HIV-infected women

    Directory of Open Access Journals (Sweden)

    Iwens Moreira de Faria

    2008-09-01

    alta, pode-se confiar no resultado positivo, ou seja, quando a citologia for positiva para o HPV, ele certamente estará presente. A baixa sensibilidade da citologia não a qualifica como exame de rastreamento para a detecção do HPV, nesse grupo de mulheres.PURPOSE: to verify the accuracy of uterine cervix cytology for HPV diagnosis, as compared to polymerase chain reaction (PCR in samples of women with HIV. METHODS: 158 patients who had undergone a first collection of material from the uterine cervix with Ayre's spatula for PCR were included in the study. Then, another collection with Ayre's spatula and brush for oncotic cytology was performed. Only 109 slides were reviewed, as 49 of them had already been destructed for have being filed for over two years. RESULTS: the prevalence of HPV was 11% in the cytological exam and 69.7% in the PCR. Age varied from 20 to 61 years old, median 35 years. The HIV contagious route was heterosexual in 91.8% of the cases, and 79.1% of the patients had had from one to five sexual partners along their lives. The most frequent complaint was pelvic mass (5.1%, and 75.3% of the women had looked for the service for a routine medical appointment. The categorical variable comparison was done through contingency tables, using the χ2 test with Yates's correction to compare the ratios. The Fisher's test was used when one of the expected rates was lower than five. In the comparison of diagnostic tests, sensitivity, specificity and similarity ratios have been calculated. Among the 76 patients with HPV, detected by PCR, only 12 had the diagnosis confirmed by cytology (sensitivity=15.8%, which on the other hand did not present any false-positive results (specificity=100%. Concerning the HPV presence, the cytological prediction for positive results was 100% and 33.3% for negative, when both results were compared. Among the 12 patients with HPV positive cytology, four (33.3% presented cervical intraepithelial neoplasia (OR=56; positive similarity

  20. "Primary Squamous Cell Carcinoma of the Vagina: HPV Detection, p16INK4A Overexpression and Clinicopathological Correlations"

    OpenAIRE

    Fuste, Victoria; Del Pino, Marta; Perez, Assumpta; Garcia, Angel; Torne, Aureli; Pahisa, Jaume; Ordi, Jaume

    2010-01-01

    Abstract Aim: To determine the role of HPV in the pathogenesis of primary squamous cell carcinoma of the vagina (SCCVa), and to evaluate its clinico-pathological significance. Methods: All cases of SCCVa diagnosed over a 15 year period from two hospitals in Barcelona, Spain (n=32) were retrieved. Patients with a history of carcinoma of the cervix diagnosed less than 5 years before were excluded. HPV was detected and typed by PCR using the SPF10 primers. Immunohistochemistry for...

  1. Experiment list: SRX129065 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available EMBRYO IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX UTER...I (the neck) opening into VAGINA. Beyond the isthmi at the upper abdominal end of

  2. Experiment list: SRX314628 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX UTERI (the ...neck) opening into VAGINA. Beyond the isthmi at the upper abdominal end of fundus

  3. Experiment list: SRX508008 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available te of EMBRYO IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX... UTERI (the neck) opening into VAGINA. Beyond the isthmi at the upper abdominal e

  4. Experiment list: SRX129063 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available te of EMBRYO IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX... UTERI (the neck) opening into VAGINA. Beyond the isthmi at the upper abdominal e

  5. Experiment list: SRX508009 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available te of EMBRYO IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX... UTERI (the neck) opening into VAGINA. Beyond the isthmi at the upper abdominal e

  6. Experiment list: SRX314629 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available te of EMBRYO IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX... UTERI (the neck) opening into VAGINA. Beyond the isthmi at the upper abdominal e

  7. Experiment list: SRX314630 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available te of EMBRYO IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX... UTERI (the neck) opening into VAGINA. Beyond the isthmi at the upper abdominal e

  8. Experiment list: SRX336292 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available EMBRYO IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX UTER...I (the neck) opening into VAGINA. Beyond the isthmi at the upper abdominal end of

  9. Experiment list: SRX129064 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available te of EMBRYO IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX... UTERI (the neck) opening into VAGINA. Beyond the isthmi at the upper abdominal e

  10. Experiment list: SRX314627 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX UTERI (the ...neck) opening into VAGINA. Beyond the isthmi at the upper abdominal end of fundus

  11. Experiment list: SRX336290 [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX UTERI (the n...eck) opening into VAGINA. Beyond the isthmi at the upper abdominal end of fundus,

  12. A systematic overview of radiation therapy effects in cervical cancer (cervix uteri).

    Science.gov (United States)

    Einhorn, Nina; Tropé, Claes; Ridderheim, Mona; Boman, Karin; Sorbe, Bengt; Cavallin-Ståhl, Eva

    2003-01-01

    A systematic review of radiation therapy trials in several tumour types was performed by The Swedish Council of Technology Assessment in Health Care (SBU). The procedures for evaluation of the scientific literature are described separately (Acta Oncol 2003; 42: 357-365). This synthesis of the literature on radiation therapy for cervical cancer is based on data from 1 meta-analysis and 34 randomized trials. In total, 35 scientific articles are included, involving 7 952 patients. The results were compared with those of a similar overview from 1996 including 34 024 patients. The conclusions reached can be summarized in these points: There are limited scientific data supporting that postoperative pelvic radiotherapy improves disease-free survival in early cervical cancer. No firm conclusion can be drawn. There is moderate scientific evidence that external beam radiotherapy combined with brachytherapy gives a similar disease-free and overall survival rate as radical hysterectomy in early cervical cancer. There is strong scientific evidence that concomitant radiochemotherapy improves disease-free and overall survival compared to radiotherapy alone in early cervical cancer. The NCI has recently published an announcement stating that cisplatin-based chemotherapy should be used concomitantly with radiotherapy in cervical cancer. No solid documentation for this statement can be found concerning locally advanced stages ( >IIB). There is a strong scientific evidence that cisplatin-based chemotherapy given concomitantly with radiotherapy is superior to concomitant chemotherapy with hydroxyurea. There is no scientific evidence to show that neoadjuvant chemotherapy followed by radiotherapy improves disease-free or overall survival compared to radiotherapy alone in patients with localized cervical cancer. There is moderate scientific evidence that high-dose-rate brachytherapy gives the same local control rate as low-dose-rate brachytherapy but with fewer rectal complications.

  13. PREVENTION OF CANCER OF THE CERVIX UTERI AT AN ANTENATAL CLINIC

    Directory of Open Access Journals (Sweden)

    I. E. Bakhlaev

    2009-01-01

    Full Text Available Cervical cancer (CC morbidity is analyzed on the basis of the data of the Karelian cancer register over the period 1998-2007. During this period, 816 cases of CC were registered and 126 were found at an antenatal clinic (AC. Its early detection rate was ascertained to be 96% during screening at the AC. A comprehensive examination was made in 1742 women with various cervical diseases, of them 37.5% were infected with human papillomavirus (HPV. High-grade dysplasia and carcinoma in situ were diagnosed in 6.6% of the HPV-infected patients. Large-scale screening for HPV infection and pretumor disorders with their further treatment will aid in reduc- ing CC morbidity and mortality rates.

  14. [Incrustation of marker threads of intrauterine devices in the uterine cavity and cervix uteri].

    Science.gov (United States)

    Patai, K; Berényi, M; Gimes, G

    1986-01-01

    If the indicating thread of intrauterine contraceptive devices is situated inside the uterine cavity, it is incrustated in the same way with calcium carbonate like the device itself. In 20 per cent the intracervical part of the thread is incrustated, too. The danger of an ascending inflammatory disease is increasing with the precipitation of substances, probably, denaturated fibrine or mucine according to the infrared spectral analysis.

  15. Conization of the cervix uteri. Complications in connection with plain catgut or silk suturing

    DEFF Research Database (Denmark)

    Holmskov, A; Qvist, N; Møller, A

    1984-01-01

    During a retrospective study on postoperative complications in 213 patients who had undergone conization, a (non-significant) reduction in the bleeding rate from 27.9% to 18.6% was found when using silk sutures (102 patients) instead of plain catgut (111 patients) for adaption of the edges of the...

  16. CYTO-HISTOPATHOLOGICAL CORRESPONDENCES IN INTRAEPITHELIAL LESIONS OF THE CERVIX UTERI

    Directory of Open Access Journals (Sweden)

    Nicoleta Simion

    2015-11-01

    Full Text Available Our research focused on the evaluation of the correlation degree between the cytological and histopathologicalexam, with the aim to identify the advantages and limitations in the application of the two methods. The study groupconsisted of 28 patients diagnosed by conventional Papanicolaou cytology and routine histopathology exam. The resultsshowed a correspondence between diagnoses with the association of benign cervical, uterine, and ovarian pathologies,sometimes with an upgrade of the intraepithelial lesions in histopathological exam. Conventional and liquid basedcytology represent two accessible and well tolerated methods of diagnosis as well as of post-therapy monitorization. Thehistopathological examination is absolutely mandatory for confirmation of the diagnosis, for upgrade of intraepithelialhigh grade lesions to carcinoma, and for initiation of any therapeutic scheme.

  17. Reconstruction of congenital defects of the vagina.

    Science.gov (United States)

    Eldor, Liron; Friedman, Jeffrey D

    2011-05-01

    Congenital absence of the vagina is a relatively rare condition most commonly associated with Mayer-Rokitansky-Kuster-Hauser (MRKH) syndrome. Historically, several reconstructive techniques have been described to provide for functional vaginal reconstruction on these patients, both operative and nonoperative. Although there are many advantages and disadvantages of the various procedures, one experience with the use of split thickness skin grafts to reconstruct the vagina has produced acceptable functional results with limited donor site morbidity. Careful planning and timing of this form of reconstruction can produce predictable results in patients who are nearing sexual maturity.

  18. Aggressive approach in a case of cancer cervix with uremia

    Directory of Open Access Journals (Sweden)

    M G Janaki

    2010-01-01

    Full Text Available Carcinoma of cervix is the most common cancer in developing countries. Majority of them present in locally advanced stages. A 36-year-old lady presented with bleeding and white discharge per vagina since four months, vomiting and reduced urine output since two weeks. Patient had an exophytic cervical growth. Investigation revealed elevated serum creatinine. Patient received single fraction radiation and underwent percutaneous nephrostomy. At one month follow-up, serum creatinine returned to almost normal level. Patient underwent bilateral ante grade stenting and completed concurrent chemoradiotherapy. In selected subsets of patients, aggressive management offered longer palliation and good quality of life.

  19. Primary primitive neuroectodermal tumor of the cervix

    Directory of Open Access Journals (Sweden)

    Li B

    2013-06-01

    Full Text Available Bo Li,1 Ling Ouyang,1 Xue Han,1 Yang Zhou,1 Xin Tong,1 Shulang Zhang,1 Qingfu Zhang21Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, 2Department of Pathology, the First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, People’s Republic of ChinaAbstract: Primary primitive neuroectodermal tumors (PNETs are rare and high-grade malignant tumors that mostly occur in children and young adults. The most common sites are the trunk, limbs, and retroperitoneum. Herein, we present a case of a PNET involving the cervix uteri in a 27-year-old woman. The lesion showed characteristic histologic features of a PNET and was positive for the immunohistochemical markers cluster of differentiation (CD 99, vimentin, neuron-specific enolase, neural cell adhesion molecule 1 (CD56, and CD117 (c-kit, further defining the tumor while helping to confirm PNET. The clinical Stage IIIB tumor was treated with chemotherapy and radiotherapy.Keywords: primitive neuroectodermal tumor, PNET, cervical neoplasm, immunohistochemistry

  20. Lymphoma of the Cervix

    Directory of Open Access Journals (Sweden)

    Juanita Parnis

    2012-01-01

    Full Text Available Primary non-Hodgkins lymphoma of the uterine cervix is a very rare diagnosis. A 54-year-old woman presented with a 3-month history of postmenopausal bleeding per vaginum. On examination, a friable, fungating lesion was seen on the cervix. Histology revealed a CD 20 positive high-grade non-Hodgkin’s diffuse large B cell lymphoma from cervical biopsies and endometrial curettage. She was diagnosed as stage IE after workup and subsequently treated with six cycles of R-CHOP chemotherapy followed by radiotherapy of the involved field.

  1. Linfoma no Hodgkin primario de vagina

    OpenAIRE

    Guldrís N,Eva María; Vázquez C,María Pía; Carballo N,Elba; Porto Q,Magdalena; Heliodoro Alba O,Luis; Iglesias R,Begoña

    2013-01-01

    La afectación primaria del tracto genital femenino de los linfomas no Hodgkin es poco frecuente (2% de los linfomas primarios extraganglionares). Los órganos más afectados son los ovarios seguidos del cérvix, siendo la localización endometrial y vaginal extremadamente rara. Presentamos el caso de una paciente de 44 años diagnosticada de linfoma primario de vagina, estadio IE A, con remisión completa tras tratamiento combinado quimioterápico y quirúrgico. Es importante tener presente estas rar...

  2. 8-Chloro-cAMP-Related Changes on Mice Uteri

    Directory of Open Access Journals (Sweden)

    Andrea Actis

    2002-01-01

    Full Text Available Histopathological effects of cAMP analog (8-Chloro-cAMP, tamoxifen, and medroxyprogesterone, alone or combined, upon BALB/c mice uteri are reported. 8-Chloro-cAMP diminished uterine weight, but did not modify its histopathology or estral cycle significantly. Tamoxifen diminished uterine weight showing cystic hyperplasia and an estral cycle arrested at diestrus. Medroxyprogesterone increased uterine weight, caused a swelling of the endometrium and a pseudopregnancy estrus. When combined with 8-Chloro-cAMP, tamoxifen or medroxyprogesterone always had a predominant effect. We concluded that the effects of 8-Chloro-cAMP on mice uteri did not cause significant changes on its histopathology, but diminished its weight.

  3. Lichen planus of uterine cervix - the first report of a novel site of occurrence: a case report

    OpenAIRE

    Gupta, Ruchika; Bansal, Bhavna; Singh, Sompal; Yadav, Indra; Gupta, Kusum; Kudesia, Madhur

    2009-01-01

    Introduction Lichen planus is an immune mediated inflammatory lesion involving skin and mucosal sites including oral mucosa, vulva and rarely vagina. Lichen planus occurring at mucosal sites has been shown to be associated with squamous cell carcinoma in a proportion of cases. To the best of our knowledge, no case of lichen planus of uterine cervix has been reported in the available literature. Case Presentation A 45-year-old female underwent vaginal hysterectomy for uterine prolapse. The res...

  4. Agenésia da Vagina

    OpenAIRE

    1998-01-01

    Nos últimos dez anos foram tratados dez doentes com o diagnóstico de agenésia da vagina. A reconstrução foi realizada com recurso à técnica de McIndoe. Faz-se a análise retrospectiva dos doentes tratados, com referência ao diagnóstico (S. Mayer-Rokitansky-7, S. Androgenital-1, S. Testículo feminizante-2), sua classificação, tratamento, complicações e resultados. Na opinião dos autores a técnica de McIndoe é a mais indicada para o tratamento destas malformações, pela sua simplicidade, baixa mo...

  5. Familial myomatosis cutis et uteri, segmental type 2

    Directory of Open Access Journals (Sweden)

    Palak Deshmukh

    2013-01-01

    Full Text Available Reed′s syndrome or familial myomatosis cutis et uteri, an autosomal dominant inherited condition with incomplete penetrance, is characterized by multiple cutaneous and uterine leiomyomas. [1] Uterine leiomyomas usually commence earlier compared to that in the general population and cutaneous leiomyomas may precede, follow or occur concurrently. Few patients may have associated renal cell carcinoma. Herein we report a case of a 50-year-old female with multiple, painful cutaneous leiomyomas and who had undergone hysterectomy owing to large uterine fibroids. Her 18-year-old daughter also has uterine fibroids.

  6. Ureterlaesion ved radikal hysterektomi for cancer colli uteri

    DEFF Research Database (Denmark)

    Rolff, M; Bang, T; Bostofte, E

    1996-01-01

    Eight ureter lesions of the ureter occurred among 100 consecutive patients undergoing radical hysterectomy for cancer of the uterine cervix. The management of this problem is discussed, and treatment guidelines for the most commonly seen lesions are proposed. It is concluded that a more liberal u...

  7. Primary uterine cervix melanoma resembling malignant peripheral nerve sheath tumor: a case report.

    Science.gov (United States)

    Pusceddu, Sara; Bajetta, Emilio; Buzzoni, Roberto; Carcangiu, Maria Luisa; Platania, Marco; Del Vecchio, Michele; Ditto, Antonino

    2008-10-01

    A rare variant of malignant melanoma (MM) of the uterine cervix that mimics a malignant peripheral nerve sheath tumor (MPNST) is described. A 43-year-old white woman was admitted to the hospital complaining of genital discharge and vaginal bleeding. Neoadjuvant chemotherapy and total abdominal hysterectomy and bilateral salpingo-ovariectomy plus pelvic lymphadenectomy were performed, and the diagnosis was MPNST, FIGO IIB. Pathological examination showed a diffuse proliferation of amelanotic spindle cells and large, highly atypical, frequently multinucleated, bizarre, and S100-, HMB-45-, vimentin-positive cells. The patient remained disease-free for 43 months, when an abdominal computed tomographic scan showed local polypoid vaginal lesions, with histological features of typical MM. A pathological review was obtained in our institution by a gynecological pathologist, who defined the primary neoplasm in the cervix as an MM, with a pattern of growth histologically simulating an MPNST, metastatic to the vagina. To our knowledge, this is the first report in literature of MM of the uterine cervix resembling MPNST. Despite its rarity, this variant of MM should be considered when a diagnosis of cervix MPNST is made. The histological and immunohistochemical features of these different entities should be considered in the differential diagnosis.

  8. Embryonal rhabdomyosarcoma of the cervix

    Directory of Open Access Journals (Sweden)

    Ocheke A

    2011-01-01

    Full Text Available Embryonal rhabdomyosarcoma (sarcoma botyroides of the cervix, which is rare, is described in a 16-year-old. The combined use of chemotherapy, radiotherapy and surgery has markedly improved survival in those with this condition. However, our patient did not benefit from this treatment modality due to late presentation and loss to follow-up.

  9. ULTRASONOGRAPHIC EVALUATION OF PREGNANCY WITH BLEEDING PER VAGINA

    Directory of Open Access Journals (Sweden)

    Amit C

    2014-12-01

    Full Text Available This study of 150 patients presenting with bleeding per vagina in first, second & third trimester was conducted to evaluate the cause of bleeding, access the severity of condition, to predict the later coarse of pregnancy & to evaluate the role of ultrasonography in management. This study was conducted in department of Radiology at Basaweshwar Teaching & General hospital, Government general hospital & Sangameshwer teaching & General hospital. This study concludes that, Ultrasonography should be the first line of investigation in all the cases of bleeding per vagina in pregnancy

  10. Audit on preinvasive and invasive neoplasm of the cervix and associated pathologies among the women with uterine prolapse in rural women of North India

    Directory of Open Access Journals (Sweden)

    Seema Dayal

    2016-01-01

    Full Text Available Background: Uterine prolapse is a common complaint in gynecology. It is defined as an abnormal downward displacement or protrusion of the pelvic structures in the vaginal canal. Aim: The aim of this study was to know the gross and microscopic cervical changes in uterine prolapse and their association with clinical findings. Materials and Methods: The present study was conducted in the histopathology section of the Department of Pathology in Rural Institute of Medical Science and Research, Saifai, Etawah, Uttar Pradesh, on hysterectomy specimens with prolapse uterus from January 2012 to May 2015. Results: The minimum age of patients with prolapse was 25 years and maximum age was 70 years, maximum number of patients belonged to 41–50 years age group (38.72%. Common clinical complaint was something coming out per vagina (53.61%. On gross examination, hypertrophy of cervix was a common pathology (46.38%. Histopathology examination of prolapse uterus showed chronic cervicitis (87.65%, cervical intraepithelial neoplasia I (21.70%, and others, but carcinoma cervix was not present. Conclusion: Prolapse uterus was a common gynecological complaint among rural women, usually clinically presenting with something coming out per vagina. Grossly, the cervix appeared hypertrophied in the majority. Histopathological examination showed chronic cervicitis in bulk. Chronic cervicitis paves the way for premalignant and malignant lesions of cervix, though cervical carcinoma is rare in prolapse uterus. Prolapse uterus must be diagnosed early so as to provide early treatment before the complications arise.

  11. Unusual migration of ventriculo peritoneal distal catheter into vagina

    Directory of Open Access Journals (Sweden)

    Sghavamedin Tavallaee

    2015-04-01

    Full Text Available VP shunt is one of the most popular methods for ICP reduction and treatment of hydrocephalus. Various complications of this method are not uncommon such as shunt malfunction, infection and unusual migration of distal catheter. I present a case of migration of the peritoneal catheter out of the vagina.

  12. SPINDLE CELL SARCOMA OF VAGINA- A CASE REPORT

    Directory of Open Access Journals (Sweden)

    Sankareswari

    2013-04-01

    Full Text Available ABSTRACT : Malignant tumors of vagina are rare accounting for 1 to 4 % of all genital malignancies. Rarest of rare is spindle cell sarcoma of vagina having a very poor prognosis. 47yrs old, regularly menstruating woman, completed f amily, presented with hard painful ulcerated swelling near the vaginal introitus and l ower vaginal wall of 4 months duration. On examination, 2x4cms sized ulcerated growth with exc avated base, covered with necrotic material, present in the left antero-lateral lower e nd of vagina, inner to hymenal ring which was tender, hard, indurated, infiltrating, fixed to bas e and did not bleed to touch. Another nodule of size 1x1cm tender, hard, fixed and necrotic was pres ent below the external urethral meatus. HPE revealed Amelanotic malignant melanoma of vagin a. Immunohistochemistry revealed Spindle cell sarcoma of vagina. Tumor board recommen ded neo-adjuvant chemotherapy and radiotherapy (CT + EBRT. Of the recommended 50Gy, s he completed 46Gy in 23days and 1 course of chemotherapy (VAC. On review after 9 mont hs, the lesion disappeared clinically and she was advised to complete the treatment. Inspite o f radio and chemotherapy secondaries to lungs and brain could not be prevented and the patien t expired 20 months after the final diagnosis

  13. Adenocarcinoma of the uterine cervix.

    Science.gov (United States)

    Rutledge, F N; Galakatos, A E; Wharton, J T; Smith, J P

    1975-05-01

    From January 1, 1947, through December 31, 1971, 219 patients with primary adenocarcinoma of the intact uterine cervix were treated at the M.D. Anderson Hospital and Tumor Institute. Two modes of therapy were primarily used, namely, irradiationtherapy alone and irradiation therapy plus operation. The 5 year survival resultsare 83.7 per cent for patients with Stage i disease, 48.0 per cent for patients with Stage ii disease, 29.2 per cent for patients with Stage iii disease, and 0.0 per cent for patients with Stage iv disease. The group with irradiation plus operation had a better over-all survival rate. In addition, the incidence of central and pelvic recurrent disease was remarkably lower (fourfold difference). The urologic and bowel complications are discussed. This review lends support for our practice of preoperative irradiation followed by simple (constructive) hysterectomy for selected patients eith adenocarcinoma of the uterine cervix.

  14. [Update on current care guidelines. Diagnosis, treatment and follow-up of cytological changes in the cervix, vagina and vulva].

    Science.gov (United States)

    Pekka, Nieminen; Anttila, Ahti; Bützow, Ralf; Heikkilä, Elina; Hiltunen-Back, Eija; Mäenpää, Johanna; Puistola, Ulla; Rantanen, Virpi; Rintala, Marjut; Räisänen, Ilkka; Santalahti, Anne; Talvensaari-Mattila, Anne; Vartiainen, Juhani; Vuento, Maarit; Yliskoski, Merja

    2010-01-01

    Approximately 150 cervical cancer cases are diagnosed in Finland annually. Both incidence and mortality have decreased by 80% since organised screening began. Recently, screening based on primary HPV-testing with Pap-smear triage has been shown to be more sensitive and more specific among women over 35 years old in randomised studies and thus may be implemented in routine. Abnormal findings in Pap smears indicate management. Confirmed CIN1 lesions are followed up and CIN2 and worse lesions treated. Follow-up after treatment should be reliably arranged, because elevated risk of cancer remains over 20 years after treatment. Quality control is of utmost importance.

  15. Management of radionecrosis of the vulva and distal vagina

    Energy Technology Data Exchange (ETDEWEB)

    Roberts, W.S.; Hoffman, M.S.; LaPolla, J.P.; Ruas, E.; Fiorica, J.V.; Cavanagh, D. (Univ. of South Florida, Tampa (USA))

    1991-05-01

    Twelve patients were seen between January 1983 and June 1989 with the clinical diagnosis of radionecrosis of the vulva or distal vagina. Seven patients received radiation for vulvar cancer, three for distal vaginal cancer, and two for recurrent endometrial cancer. No patient healed spontaneously and the mean delay in surgical therapy was 8.5 months. The radionecrotic site was treated with local therapy, radical local excision (with or without colostomy), or exenteration. The operative defect was closed primarily in three patients and covered with local flaps or myocutaneous flaps in seven patients. The two patients with local care still have radionecrotic ulcers. One of three patients who were closed primarily continues to have an ulcer. All other patients have healed satisfactorily except one who died after two attempts to correct the problem. Radionecrosis of the vulva and distal vagina should generally be treated surgically.

  16. Hernia uteri inguinalis in a case of ovotesticular disorder of sexual differentiation.

    Science.gov (United States)

    Venkataram, Aniketh; Shivaswamy, Sadashivaiah; Babu, Rajashekara; Santhosh, Shivashankar

    2013-02-01

    An 18-year-old phenotypic male presented with an irreducible left inguinal mass, gynecomastia, and hypospadias. This mass on exploration was found to be a nonfunctional uterus with ipsilateral ovary and was excised. Further investigation confirmed the presence of a contralateral testis and a genotype of 46, XX. This confirmed the diagnosis of ovotesticular disorder of sexual differentiation (formerly true hermaphroditism) with obstructed hernia uteri inguinalis. The patient was raised as a male. Subcutaneous mastectomy for gynecomastia and neourethra construction with full thickness skin graft for hypospadias were performed. Hernia uteri inguinalis is rarely seen in this condition with only 2 cases being reported worldwide thus far, including our case.

  17. Intensity modulated whole pelvic radiotherapy in patients with cervix cancer: analysis of acute toxicity

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Young Min; Lee, Hyung Sik; Hur, Won Joo; Cha, Moon Seok; Kim, Hyun Ho [School of Medicine, Dong-A University, Busan (Korea, Republic of)

    2006-12-15

    To evaluate acute toxicities in cervix cancer patients receiving intensity modulated whole pelvic radiation therapy (IM-WPRT). Between August 2004 and April 2006, 17 patients who underwent IM-WPRT were analysed. An intravenous contrast agent was used for radiotherapy planning computed tomography (CT). The central clinical target volume (CTV) included the primary tumor, uterus, vagina, and parametrium. The nodal CTV was defined as the lymph nodes larger than 1 cm seen on CT and the contrased-enhanced pelvic vessels. The planning target volume (PTV) was the 1-cm expanded volume around the central CTV, except for a 5-mm expansion from the posterior vagina, and the nodal PTV was defined as the nodal CTV plus a 1.5 cm margin. IM-WPRT was prescribed to deliver a dose of 50 Gy to more than 95% of the PTV. Acute toxicity was assessed with common toxicity criteria up to 60 days after radiotherapy. Grade 1 nausea developed in 10 (58.9%) patients, and grade 1 and 2 diarrhea developed in 11 (64.7%) and 1 (5.9%) patients, respectively. No grade 3 or higher gastrointestinal toxicity was seen. Leukopenia, anemia, and thrombocytopenia occurred in 15 (88.2%). 7 (41.2%), and 2 (11.8%) patients, respectively, as hematologic toxicities. Grade 3 leukopenia developed in 2 patients who were treated with concurrent chemoradiotherapy. IM-WPRT can be a useful treatment for cervix cancer patients with decreased severe acute toxicities and a resultant improved compliance to whole pelvic irradiation.

  18. Pelvic Myxoid Leiomyoma Mass between Vagina and Rectum

    OpenAIRE

    Omar AlShalabi; Fadi Obaied Alahmar; Hazem Aljasem; Bayan Alsaid; Abdulghani AlShalabi

    2016-01-01

    Leiomyomas are the most common pelvic tumors in women. About 20–30% of women older than 35 are affected. Rare conditions of leiomyomas have extrauterine locations. Myxoid degeneration is a rare type of leiomyoma degeneration. We report a case of solid-cystic myxoid leiomyoma in a 53-year-old woman complained of constipation, urinary hesitation, and malodorous vaginal discharge with palpable 17 × 12 cm mass between vagina and rectum. Regarding the inferior location of the mass, a perineal appr...

  19. Pelvic Myxoid Leiomyoma Mass between Vagina and Rectum

    Science.gov (United States)

    Alahmar, Fadi Obaied; Aljasem, Hazem; Alsaid, Bayan; AlShalabi, Abdulghani

    2016-01-01

    Leiomyomas are the most common pelvic tumors in women. About 20–30% of women older than 35 are affected. Rare conditions of leiomyomas have extrauterine locations. Myxoid degeneration is a rare type of leiomyoma degeneration. We report a case of solid-cystic myxoid leiomyoma in a 53-year-old woman complained of constipation, urinary hesitation, and malodorous vaginal discharge with palpable 17 × 12 cm mass between vagina and rectum. Regarding the inferior location of the mass, a perineal approach was used to enucleate it. This rare location has not been mentioned before. The woman was finally diagnosed by pathologists which was myxoid leiomyoma. PMID:27429825

  20. Pelvic Myxoid Leiomyoma Mass between Vagina and Rectum

    Directory of Open Access Journals (Sweden)

    Omar AlShalabi

    2016-01-01

    Full Text Available Leiomyomas are the most common pelvic tumors in women. About 20–30% of women older than 35 are affected. Rare conditions of leiomyomas have extrauterine locations. Myxoid degeneration is a rare type of leiomyoma degeneration. We report a case of solid-cystic myxoid leiomyoma in a 53-year-old woman complained of constipation, urinary hesitation, and malodorous vaginal discharge with palpable 17×12 cm mass between vagina and rectum. Regarding the inferior location of the mass, a perineal approach was used to enucleate it. This rare location has not been mentioned before. The woman was finally diagnosed by pathologists which was myxoid leiomyoma.

  1. Congenital absence of uterine cervix

    Directory of Open Access Journals (Sweden)

    Selvaraj Ravi Lakshmy

    2016-10-01

    Full Text Available Cervical agenesis or dysgenesis is an extremely rare congenital anomaly. Patients with congenital absence of the cervix present with primary amenorrhea and infertility. Though it poses a diagnostic challenge to the clinician, correct diagnosis prior to surgery is possible with the help of ultrasound. Early diagnosis offers significant advantages in patient care and effective presurgical planning. This case report reviews two cases of cervical agenesis diagnosed with the help of ultrasound and later confirmed with the help of MRI. Ultrasonography is the modality of choice to define the internal genital anatomy and helps us to classify the level of obstruction or aplasia in obstructive uterine anomalies. [Int J Reprod Contracept Obstet Gynecol 2016; 5(10.000: 3634-3636

  2. KANDIDIASIS VAGINA YANG MENDAPAT TERAPI SISTEMIK DAN TOPIKAL: SEBUAH LAPORAN KASUS

    Directory of Open Access Journals (Sweden)

    Dewa Made Rendy Sanjaya

    2014-08-01

    Full Text Available Kandidiasis vulvovaginitis ialah penyakit jamur candida yang mengenai mukosa vagina danvulva.Penyebabnya yang tersering biasanya adalah candida albicans.Gejala klinis KandidiasisVulvovaginitis ialah gatal didaerah vulva, dan pada yang berat terdapat rasa panas,dispaneuria.lesi eritema, hiperemis dilabia mayora, dan vagina 1/3 bawah. Dilaporkan satu  kasusseorang wanita. berumur 26 tahun dengan keluhan keputihan pada daerah vagina sertamengalami kegatalan dan rasa nyeri terjadi sejak 2 minggu yang lalu. Pada gambaran klinis tampak dinding vagina eritema, erosi dengan secret putih kental. Pada pengecatan gram darisecret mukosa vagina didapatkan leukosit blastopsora dan pseudohifa. Pemerikaan KOH 10%dari sekret vagina didapatkan blastopore dan pesudohifa.Penanganan yang diberikan adalah obatsistemik dengan flukonazol 1x150 mg dosis tunggal dan topical diberikan ketokenazol cream.Prognosis kasus tersebut adalah baik..

  3. Adjuvant postoperative radiation therapy for carcinoma of the uterine cervix

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Kyung Ja; Moon, Hye Seong; Kim, Seung Cheol; Kim, Chong Il; Ahn, Jung Ja [College of Medicine, Ewha Womans Univ., Seoul (Korea, Republic of)

    2003-09-01

    This study was undertaken to evaluate the efficacy of postoperative radiotherapy, and to investigate the prognostic factors for FIGO stages IB-IIB cervical cancer patients who were treated with simple hysterectomy, or who had high-risk factors following radical hysterectomy and pelvic lymph node dissection. Between March 1986 and December 1998, 58 patients, with FIGO stages IB-IIB cervical cancer were included in this study, The indications for postoperative radiation therapy were based on the pathological findings, including lymph node metastasis, positive surgical margin, parametrial extension, Iymphovascular invasion, invasion of more than half the cervical stroma, uterine extension and the incidental finding of cervix cancer following simple hysterectomy. All patients received external pelvic radiotherapy, and 5 patients, received an additional intracavitary radiation therapy. The radiation dose from the external beam to the whole pelvis was 45 - 50 Gy. Vagina cuff irradiation was performed, after completion of the external beam irradiation, al a low-dose rate of CS-137, with the total dose of 4488-4932 chy (median: 4500 chy) at 5 mm depth from the vagina surface. The median follow-up period was 44 months (15-108 months), The 5-yr actuarial local control rate, distant free survival and disease-free survival rate were 98%, 95% and 94%, respectively. A univariate analysis of the clinical and pathological parameters revealed that the clinical stage (p=0.0145), status of vaginal resection margin (p=0.0002) and parametrial extension (p=0.0001) affected the disease-free survival. From a multivariate analysis, only a parametrial extension independently influenced the disease-free survival. Five patients (9%) experienced Grade 2 late treatment-related complications, such as radiation proctitis (1 patient), cystitis (3 patients) and lymphedema of the leg (1 patient). No patient had grade 3 or 4 complications. Our results indicate that postoperative radiation therapy can

  4. LASER EN EL TRATAMIENTO DE LA NEOPLASIA INTRAEPITELIAL DE VAGINA

    OpenAIRE

    Yazigi I.,Roberto; Rodríguez A.,Tulio; Contreras M,Luis

    2006-01-01

    Objetivo: Presentar la experiencia en el tratamiento de la neoplasia intraepitelial de vagina (VAIN) utilizando el láser de CO2. Método: 19 pacientes con el diagnóstico histológico de VAIN fueron tratadas con esta técnica. Se utilizó un diámetro del punto de helio-neón de 1,5 a 2 mm y un poder de densidad de 400 a 500 W/cm². Toda la lesión detectable más un margen de al menos 5 mm de tejido circundante aparentemente normal fueron vaporizados a una profundidad de 2 mm. Resultados: 12 de las 14...

  5. Real-time Elastografie: Erste Messungen von gewebeelastischen Eigenschaften der Zervix uteri

    OpenAIRE

    Burger, Katharina Adriana

    2014-01-01

    Introduction: in this study were investigated aged and duration of pregnancy dependend tissue elastic properties and changes of the normal cervix and in pregnant women. The normal findings were compared with the results in a group of patients with focal pathology of the cervix and with cervical insufficiency. Materials and Methods: a total of 165 patients, among them 52 pregnant women, underwent transvaginal ultrasound using a 9 MHz high-end US device with a vaginal probe. The examiner pla...

  6. Particles translocate from the vagina to the oviducts and beyond

    Energy Technology Data Exchange (ETDEWEB)

    Wehner, A.P.; Hall, A.S.; Weller, R.E.; Lepel, E.A.; Schirmer, R.E.

    1985-03-01

    To investigate whether particles deposited in the vagina translocate to the oviducts, 0.3 ml of a 4% bone black suspension was deposited in the posterior vaginal fornix of each of five cynomolgus monkeys (Macaca fascicularis) during their mid-menstrual cycle. Simultaneously, each animal received 10 units of oxytocin by intramuscular injection. The oviducts of three animals were removed 1 hr after administration of the bone black, while those of the remaining two animals were removed 72 hr after dosing. The removed oviducts were flushed with Hank's solution and then with collagenase solution. The solutions were collected in clean vials and filtered. The filters were examined for bone black particles by light microscopy, as were filters through which solution blanks (negative controls) had been passed. Particles resembling bone black were found on all filters. There were no appreciable differences in the number or shape of these particles between the solution-blank filters and the oviduct-flush filters. The particles on both the solution-blank filters and on the oviduct-flush filters probably originated from environmental contamination by ubiquitous carbon particles. While these results suggested that no translocation took place, translocation could not be ruled out with certainty in the absence of quantitative analyses. A more definitive pilot study was then conducted with two dosed monkeys and one control, using talc labelled by neutron activation to circumvent the problem of environmental contamination. Gamma-Ray analysis of tissue and peritoneal lavage samples for the radionuclides /sup 46/Sc, /sup 59/Fe and /sup 60/Co indicated that no measurable quantities (i.e. greater than 0.5 micrograms) of talc translocated from the deposition site in the vagina to the uterine cavity and beyond.

  7. Evidence that endogenous relaxin promotes growth of the vagina and uterus during pregnancy in gilts.

    Science.gov (United States)

    Min, G; Hartzog, M G; Jennings, R L; Winn, R J; Sherwood, O D

    1997-02-01

    Recently, it was demonstrated that endogenous relaxin promotes growth of the vagina during the second half of pregnancy in rats and that administration of porcine relaxin promotes growth of the uterus in nonpregnant or early pregnant gilts. This study examined the effects of circulating relaxin on growth of both the vagina and uterus during the last two thirds of the 114-day gestation period in gilts. Furthermore, this study employed an in vitro immunohistochemical localization technique to determine whether the vagina and uterus in pigs have specific relaxin-binding sites. Three groups of pregnant gilts were used: sham-ovariectomized controls (group C; n = 8), ovariectomized progesterone-treated (group OP; n = 6), and ovariectomized progesterone- plus relaxin-treated (group OPR; n = 7). Gilts were either sham ovariectomized or ovariectomized on day 40 of gestation. Hormone replacement therapy with progesterone (group OP), progesterone plus relaxin (group OPR), or hormone vehicles (group C) began on day 38 (progesterone) or day 40 (relaxin) and continued until day 110. On day 110, the vagina and uterus were collected, and wet weight, dry weight, and percent hydration were determined. Small pieces (2-3 cm3) of the vagina and uterus from groups C and OP were frozen and cryosectioned for the immunohistochemical localization of relaxin-binding sites. Relaxin promoted growth of both the vagina and uterus. The wet weights of both the vagina and uterus in relaxin-deficient gilts (group OP) were lower (P pig. Furthermore, this study provides evidence that both the vagina and uterus contain specific and saturable relaxin-binding sites in epithelial cells, smooth muscle cells, and cells associated with blood vessels. We conclude that these cells probably initiate relaxin's effects on the vagina and uterus of the pregnant pig.

  8. In vitro effects of L-arginine on spontaneous and homocysteine-induced contractility of pregnant canine uteri.

    Science.gov (United States)

    Rizzo, Annalisa; Trisolini, Carmelinda; Spedicato, Massimo; Mutinati, Maddalena; Minoia, Giuseppe; Sciorsci, Raffaele Luigi

    2011-09-01

    The L-Arginine-Nitric Oxide Synthase-Nitric Oxide (L-Arg-NOS-NO) system exerts a pivotal role in the maintenance of uterine quiescence during pregnancy, whereas Homocysteine (Hcy) promotes uterine contractility. The aim of this study was to test the in vitro effects of L-Arg on spontaneous and Hcy-induced contractions of uteri excised from pregnant bitches. 104 strips cut from pregnant uteri were mounted in an organ bath. 40 out of 104 strips (16 from mid-gestation uteri and 24 from close to term uteri, respectively) were exposed to cumulative doses of L-Arg; 40 strips (16 from mid-gestation-uteri and 24 from close to term-uteri, respectively) were exposed to N-nitro-L-arginine methyl ester (L-NAME), a NOS antagonist; the remaining 24 strips (from close-to-term uteri) were first exposed to a single dose of Hcy and then to increasing doses of L-Arg. L-Arg showed no effects on spontaneous contractility both in mid-gestation- and close to term-uterine strips, whereas it promoted a relaxant effect on Hcy-induced contractility. On the contrary, L-NAME increased amplitude of contraction both in mid-gestation and close to term strips. These findings suggest that the L-Arg-NO system is present in the uterus of pregnant bitches and that Hcy is able to modulate its actions. Further investigation of this system may provide the basis of future obstetrical therapies in bitches.

  9. Estrogen enhances cystatin C expression in the macaque vagina.

    Science.gov (United States)

    Slayden, Ov D; Hettrich, Kevin; Carroll, Rebecca S; Otto, Lesley N; Clark, Amanda L; Brenner, Robert M

    2004-02-01

    Cystatin C is a secreted inhibitor of cysteine proteinases that participates in extracellular matrix remodeling. Whether hormones affect its expression in the vagina was unknown. Consequently, we examined the effects of estradiol (E(2)), progesterone (P), and raloxifene on vaginal cystatin C in rhesus macaques. In experiment 1, ovariectomized animals were treated sequentially with E(2) (14 d) and E(2) + P (14 d) to induce 28-d menstrual cycles. Vaginal samples were collected on d 6, 8, 14, and 28 of the induced cycle. Some cycled animals were deprived of both E(2) + P for 28 d. In experiment 2, ovariectomized animals were treated for 5 months with E(2) alone, E(2) + P, raloxifene, or left untreated. Total RNA from the vaginal wall was analyzed for the cystatin C transcript with a commercially prepared cDNA array and semiquantitative RT-PCR. Vaginal cryosections were analyzed by in situ hybridization for cystatin C transcript and by immunocytochemistry for the protein. E(2) treatment significantly (5-fold; P pelvic floor prolapse.

  10. Anatomic and histological characteristics of vagina reconstructed by McIndoe method

    Directory of Open Access Journals (Sweden)

    Kozarski Jefta

    2009-01-01

    Full Text Available Background/Aim. Congenital absence of vagina is known from ancient times of Greek. According to the literature data, incidence is 1/4 000 to 1/20 000. Treatment of this anomaly includes non-operative and operative procedures. McIndoe procedure uses split skin graft by Thiersch. The aim of this study was to establish anatomic and histological characteristics of vagina reconstructed by McIndoe method in Mayer Küster-Rockitansky Hauser (MKRH syndrome and compare them with normal vagina. Methods. The study included 21 patients of 18 and more years with congenital anomaly known as aplasio vaginae within the Mayer Küster-Rockitansky Hauser syndrome. The patients were operated on by the plastic surgeon using the McIndoe method. The study was a retrospective review of the data from the history of the disease, objective and gynecological examination and cytological analysis of native preparations of vaginal stain (Papanicolau. Comparatively, 21 females of 18 and more years with normal vaginas were also studied. All the subjects were divided into the groups R (reconstructed and C (control and the subgroups according to age up to 30 years (1 R, 1C, from 30 to 50 (2R, 2C, and over 50 (3R, 3C. Statistical data processing was performed by using the Student's t-test and Mann-Writney U-test. A value of p < 0.05 was considered statistically significant. Results. The results show that there are differences in the depth and the wideness of reconstructed vagina, but the obtained values are still in the range of normal ones. Cytological differences between a reconstructed and the normal vagina were found. Conclusion. A reconstructed vagina is smaller than the normal one regarding depth and width, but within the range of normal values. A split skin graft used in the reconstruction, keeps its own cytological, i.e. histological and, so, biological characteristics.

  11. IMPROVING THE DIAGNOSIS AND TREATMENT OF RETENTION DISORDERS OF THE UPPER URINARY TRACT IN PATIENTS WITH STAGES IIB–III CANCER OF THE CERVIX UTERI

    Directory of Open Access Journals (Sweden)

    A. D. Kaprin

    2014-08-01

    Full Text Available Study investigates the prevalence, diagnosis and treatment in patients with retention disorders upper urinary tract cervical cancer stage IIB III after more than 3 months after combined radiotherapy. In the apartment complex to the diagnosis of renal ultrasound and radioisotope study of renal excretory function added to the study ureteral emissions by color Doppler sonography. Information on ureteral emissions revealed a violation of the early passage of urine in 23.1 % of patients with renal ultrasound revealed no pathology. On the basis of violations ureteral emissions increase in the number of patients, respectively, are assigned to nonoperative treatment (anti-inflammatory, spasmolytic therapy. As a result, decreased by 14.2 % (p = 0.034, female patients, which showed drainage of the upper urinary tract.

  12. IMPROVING THE DIAGNOSIS AND TREATMENT OF RETENTION DISORDERS OF THE UPPER URINARY TRACT IN PATIENTS WITH STAGES IIB–III CANCER OF THE CERVIX UTERI

    Directory of Open Access Journals (Sweden)

    A. D. Kaprin

    2012-01-01

    Full Text Available Study investigates the prevalence, diagnosis and treatment in patients with retention disorders upper urinary tract cervical cancer stage IIB III after more than 3 months after combined radiotherapy. In the apartment complex to the diagnosis of renal ultrasound and radioisotope study of renal excretory function added to the study ureteral emissions by color Doppler sonography. Information on ureteral emissions revealed a violation of the early passage of urine in 23.1 % of patients with renal ultrasound revealed no pathology. On the basis of violations ureteral emissions increase in the number of patients, respectively, are assigned to nonoperative treatment (anti-inflammatory, spasmolytic therapy. As a result, decreased by 14.2 % (p = 0.034, female patients, which showed drainage of the upper urinary tract.

  13. One Case with Squamous Carcinoma of Cervix and Adenocarcinoma of Corpus Uteri%宫颈鳞癌复合宫体腺癌1例

    Institute of Scientific and Technical Information of China (English)

    金志红

    2001-01-01

    @@ 1 病例报告 患者 50岁,孕4产4,不规则阴道流血3月.当地医院做分段诊刮术,病理检查:宫颈鳞状细胞癌Ⅱ~Ⅲ级,乳头状腺癌,宫内膜鳞状细胞癌Ⅱ~Ⅲ级.妇科检查:外阴、阴道无异常.宫颈菜花状肿块,Φ5cm,质脆,触之易出血.宫体增大如孕2月大小,质硬.双侧宫旁呈片状增厚达盆壁,弹性尚可.双附件区未扪及肿块.会诊病理检查:(宫颈、宫腔)中度分化腺鳞癌.CT检查:子宫明显增大,考虑宫颈癌并宫体受侵;宫腔少量积液.入院诊断:宫颈腺鳞癌Ⅰ~Ⅱ期并宫体受侵.行60Co腔内放疗4次,A点累积量24Gy,并予增敏化疗2次,顺铂总计60mg.术中见:宫体前位,增大如50d妊娠大小,质硬,双侧主韧带呈薄片状达盆壁,腹膜后淋巴结无肿大.行子宫广泛切除+盆腔淋巴结清扫术.剖视标本:肿瘤来源于颈管11°处,宫体受侵,病灶约3~3.5cm,实质性,浸及浅肌层.病理检查:(宫颈)中分化鳞状细胞癌;(宫腔)中分化腺癌,侵犯肌壁约0.5cm(肌壁厚约2cm);盆腔各组淋巴结、阴道残端、双附件、宫旁组织未见癌转移. 2 讨论 本病例为宫颈鳞癌、其肿瘤细胞形态迥异,缺乏同源性,故均为原发癌,象这种不同器官在同一时期均发生原发癌者称为同期原发癌.据文献报道宫颈癌发生重复癌的机率为0.8%[1]及3.94%[2].而临床上宫颈鳞癌与宫体腺癌同期发生更为罕见,Eisner等[3]的一组1 556例宫颈癌中有4例为原发宫颈癌与原发宫内膜腺癌并存,其发生率占宫颈癌的0.25%. 治疗方面,因宫颈鳞癌与宫体腺癌治疗原则基本一致,以手术为主,以放射治疗、化疗为辅,宫体腺癌另辅助孕激素治疗,故两者均可兼顾.该病例术前已行半定量腔内治疗,并行子宫广泛切除+盆腔淋巴结清扫术.术后病理诊断为:宫颈鳞癌Ⅰb期;宫体腺癌ⅠbG2期.可酌情行全盆照射,剂量40~50Gy/4~6W,并辅以孕激素治疗.

  14. Frequent Promoter Methylation of CDH1, DAPK, RARB, and HIC1 Genes in Carcinoma of Cervix Uteri: Its Relationship to Clinical Outcome

    Directory of Open Access Journals (Sweden)

    Schneider Achim

    2003-05-01

    Full Text Available Abstract Background Cervical cancer (CC, a leading cause of cancer-related deaths in women worldwide, has been causally linked to genital human papillomavirus (HPV infection. Although a host of genetic alterations have been identified, molecular basis of CC development is still poorly understood. Results We examined the role of promoter hypermethylation, an epigenetic alteration that is associated with the silencing tumor suppressor genes in human cancer, by studying 16 gene promoters in 90 CC cases. We found a high frequency of promoter methylation in CDH1, DAPK, RARB, and HIC1 genes. Correlation of promoter methylation with clinical characteristics and other genetic changes revealed the following: a overall promoter methylation was higher in more advanced stage of the disease, b promoter methylation of RARB and BRCA1 predicted worse prognosis, and c the HIC1 promoter methylation was frequently seen in association with microsatellite instability. Promoter methylation was associated with gene silencing in CC cell lines. Treatment with methylation or histone deacetylation-inhibiting agents resulted in profound reactivation of gene expression. Conclusions These results may have implications in understanding the underlying epigenetic mechanisms in CC development, provide prognostic indicators, and identify important gene targets for treatment.

  15. Prognostic relevance of human papillomavirus L1 capsid protein detection within mild and moderate dysplastic lesions of the cervix uteri in combination with p16 biomarker

    DEFF Research Database (Denmark)

    Hilfrich, Ralf; Hariri, Jalil

    2008-01-01

    OBJECTIVE: To proof the prognostic relevance of HPV L1 capsid protein detection on colposcopically-guided punch biopsies in combination with p16. STUDY DESIGN: Sections of colposcopically-guided punch biopsies from 191 consecutive cases with at least 5 years of follow-up were stained with HPV L1...... capsid protein antibodies (Cytoactiv screening antibody) and a monoclonal anti-p16 antibody. Fifty sections were derived from a benign group, 91 from low-grade (cervical intraepithelial neoplasia [CIN 1]) lesions and 50 from high-grade (CIN 2 and 3) lesions. RESULTS: Overall only 16.1% of the 87 L1......-negative, p16-positive CIN lesions showed remission of the lesion compared to 72.4% of the double positive cases. None of the L1/p16 double negative CIN lesions progressed. CONCLUSION: HPV L1 capsid protein detection with Cytoactiv screening antibody seems to be a promising new tool to predict the behavior...

  16. Carcinoma cervix with fat attenuating skull metastases

    Institute of Scientific and Technical Information of China (English)

    Anuradha Kapali; Atmakuri Sateesh Kumar; Mukunda Malathi; S D Shamsundar

    2016-01-01

    Skeletal metastasis in carcinoma cervix occurs in about 0.8-23% of cases. These lesions are usually radiographically lytic. Very few cases of metastases to the skull have been identiifed, about 5 cases to the best of our knowledge. We present a case of adenosquamous cell carcinoma of cervix with fat attenuating skull metastases in a 38-year-old lady that is not reported till date. The lesion was lytic, expansile and with negative attenuation of -15 to -30 Hounsifeld units corresponding to fat.Metastases must be included in the differentials of scalp lesions. A history of recent onset of swelling and associated lytic areas in calvarium on contrast enhanced computed tomography with multiplicity can give a clue to metastatic nature of disease.

  17. Microarray analysis of inflammatory response-related gene expression in the uteri of dogs with pyometra.

    Science.gov (United States)

    Bukowska, D; Kempisty, B; Zawierucha, P; Jopek, K; Piotrowska, H; Antosik, P; Ciesiółka, S; Woźna, M; Brüssow, K P; Jaśkowski, J M

    2014-01-01

    Pyometra, which is accompanied by bacterial contamination of the uterus, is defined as a complex disease associated with the activation of several systems, including the immune system. The objective of the study was to evaluate the gene expression profile in dogs with pyometra compared with those that were clinically normal. The study included uteri from 43 mongrel bitches (23 with pyometra, 20 clinically healthy). RNA used for the microarray study was pooled to four separated vials for control and pyometra. A total of 17,138 different transcripts were analyzed on the uteri of female dogs with pyometra and of healthy controls. From 264 inflammatory response-related transcripts, we found 23 transcripts that revealed a 10- to 77-fold increased expression. Thereby, the expression of interleukin 8 (IL8), interleukin-1-beta (IL1B), interleukin 18 receptor (IL18RAP), interleukin 1-alpha (IL1A), interleukin receptor antagonist (IL1RN) and interleukin 6 (IL6) increased 77-, 20-, 17-, 13-, 13- and 11-fold, respectively. Furthermore, the expression of the calcium binding proteins S100A8 was 44-fold higher, and that of S100A12 and S100A9 37-fold, respectively, in the uteri of canines with pyometra compared with that of the controls. Moreover, the expression of the transcripts of toll-like receptors (TLR8 and TLR2), integrin beta 2 (ITGB2), chemokine ligand 3 (CCL3), semaphorin 7A (SEMA7A), CD14 and prostaglandin-endoperoxide synthase 2 (PTGS2) was increased between 10- and 18-fold. Furthermore, after using RT-qPCR we found an increased expression of AOAH, IL1A, IL8, CCL3, IL1RN and SERPINE 1 mRNAs which can be served also as markers of the occurrence of pyometra in domestic bitches. In summary, it is concluded that up-regulation of interleukins may be used as a marker of the inflammatory response in dogs with pyometra. Moreover, all of the 23 up-regulated transcripts may be novel molecular markers of the pathogenesis of canine pyometra. Several proteins--–products of these

  18. The influence of bone density on the radiotherapy of cervix cancer

    Energy Technology Data Exchange (ETDEWEB)

    Soares, M.R. [Departamento de Fisica, Universidade Federal de Sergipe, Av. Marechal Rondon s/n, 49.100-000, Rosa Elze, Sao Cristovao, SE (Brazil); Souza, D.N., E-mail: divanizi@ufs.br [Departamento de Fisica, Universidade Federal de Sergipe, Av. Marechal Rondon s/n, 49.100-000, Rosa Elze, Sao Cristovao, SE (Brazil)

    2011-10-01

    Until the 1970s the irradiated region of a patient undergoing external beam radiotherapy was considered a homogeneous volume and a regular surface, with physical characteristics similar to water. With the improvement of medical imaging equipment, it has become possible to conduct planning in radiotherapy treatment that considers the heterogeneities and irregularities of a patient's anatomy. Consequently, such technological resources have brought greater accuracy to radiotherapy. In this study, we determined the variation in the average amount of absorbed dose on the target volume and at the point of prescription treatment by comparing the doses which were calculated in a planning system considering the patient both as a homogeneous, and as a heterogeneous medium. The results showed that when we take into account the volume of the upper vagina and cervix, and consider the pelvis as a heterogeneous medium, the calculated dose was under-estimated at some points in the studied volume with respect to the dose when this region was considered homogeneous.

  19. The influence of bone density on the radiotherapy of cervix cancer

    Science.gov (United States)

    Soares, M. R.; Souza, D. N.

    2011-10-01

    Until the 1970s the irradiated region of a patient undergoing external beam radiotherapy was considered a homogeneous volume and a regular surface, with physical characteristics similar to water. With the improvement of medical imaging equipment, it has become possible to conduct planning in radiotherapy treatment that considers the heterogeneities and irregularities of a patient's anatomy. Consequently, such technological resources have brought greater accuracy to radiotherapy. In this study, we determined the variation in the average amount of absorbed dose on the target volume and at the point of prescription treatment by comparing the doses which were calculated in a planning system considering the patient both as a homogeneous, and as a heterogeneous medium. The results showed that when we take into account the volume of the upper vagina and cervix, and consider the pelvis as a heterogeneous medium, the calculated dose was under-estimated at some points in the studied volume with respect to the dose when this region was considered homogeneous.

  20. Shape priors for segmentation of the cervix region within uterine cervix images.

    Science.gov (United States)

    Lotenberg, Shelly; Gordon, Shiri; Greenspan, Hayit

    2009-06-01

    The work focuses on a unique medical repository of digital uterine cervix images ("cervigrams") collected by the National Cancer Institute (NCI), National Institute of Health, in longitudinal multiyear studies. NCI together with the National Library of Medicine is developing a unique web-based database of the digitized cervix images to study the evolution of lesions related to cervical cancer. Tools are needed for the automated analysis of the cervigram content to support the cancer research. In recent works, a multistage automated system for segmenting and labeling regions of medical and anatomical interest within the cervigrams was developed. The current paper concentrates on incorporating prior-shape information in the cervix region segmentation task. In accordance with the fact that human experts mark the cervix region as circular or elliptical, two shape models (and corresponding methods) are suggested. The shape models are embedded within an active contour framework that relies on image features. Experiments indicate that incorporation of the prior shape information augments previous results.

  1. Functional and molecular characterization of voltage-gated sodium channels in uteri from nonpregnant rats.

    Science.gov (United States)

    Seda, Marian; Pinto, Francisco M; Wray, Susan; Cintado, Cristina G; Noheda, Pedro; Buschmann, Helmut; Candenas, Luz

    2007-11-01

    We investigated the function and expression of voltage-gated Na(+) channels (VGSC) in the uteri of nonpregnant rats using organ bath techniques, intracellular [Ca(2+)] fluorescence measurements, and RT-PCR. In longitudinally arranged whole-tissue uterine strips, veratridine, a VGSC activator, caused the rapid appearance of phasic contractions of irregular frequency and amplitude. After 50-60 min in the continuous presence of veratridine, rhythmic contractions of very regular frequency and slightly increasing amplitude occurred and were sustained for up to 12 h. Both the early and late components of the contractile response to veratridine were inhibited in a concentration-dependent manner by tetrodotoxin (TTX). In small strips dissected from the uterine longitudinal smooth muscle layer and loaded with Fura-2, veratridine also caused rhythmic contractions, accompanied by transient increases in [Ca(2+)](i), which were abolished by treatment with 0.1 microM TTX. Using end-point and real-time quantitative RT-PCR, we detected the presence of the VGSC alpha subunits Scn2a1, Scn3a, Scn5a, and Scn8a in the cDNA from longitudinal muscle. The mRNAs of the auxiliary beta subunits Scbn1b, Scbn2b, Scbn4b, and traces of Scn3b were also present. These data show for the first time that Scn2a1, Scn3a, Scn5a, and Scn8a, as well as all VGSC beta subunits are expressed in the longitudinal smooth muscle layer of the rat myometrium. In addition, our data show that TTX-sensitive VGSC are able to mediate phasic contractions maintained over long periods of time in the uteri of nonpregnant rats.

  2. Cervix cancer; Cancer du col uterin

    Energy Technology Data Exchange (ETDEWEB)

    Pointreau, Y.; Ruffier Loubiere, A.; Barillot, I. [Service de radiotherapie, centre regional universitaire de cancerologie Henry-S.-Kaplan CHU de Tours, Hpital Bretonneau, 37 - Tours (France); Pointreau, Y. [Universite Francois-Rabelais de Tours, GICC, 37 - Tours (France); CNRS, UMR 6239 -Genetique, Immunotherapie, Chimie et Cancer-, 37 - Tours (France); CHRU de Tours, laboratoire de pharmacologie-toxicologie, 37 - Tours (France); Denis, F. [Centre Jean-Bernard, 72 - Le Mans (France); Barillot, I. [Universite Francois-Rabelais, 37 - Tours (France)

    2010-07-01

    Cervix cancers declined in most developed countries in recent years, but remain, the third worldwide leading cause of cancer death in women. A precise staging, based on clinical exam, an abdominal and pelvic MRI, a possible PET-CT and a possible lymph node sampling is necessary to adapt the best therapeutic strategy. In France, the treatments of tumors of less than 4 cm without nodal involvement are often based on radiotherapy followed by surgery and, whereas tumors larger than 4 cm and involved nodes are treated with concurrent chemoradiotherapy. Based on an illustrated clinical case, indications, delineation, dosimetry and complications expected with radiotherapy are demonstrated. (authors)

  3. ALMACENAMIENTO DE ESPERMATOZOIDES EN LA VAGINA DE LA LAGARTIJA VIVÍPARA SCELOPORUS TORQUATUS (SAURIA: PRHYNOSOMATIDAE)

    OpenAIRE

    Martínez-Torres, Martín

    2009-01-01

    Las hembras de muchas especies de lagartijas tienen la capacidad de almacenar espermatozoides en un sistema de túbulos especiales presentes en la vagina. Sin embargo, en otras especies los espermatozoides se almacenan en el infundíbulo. Se utilizó una técnica sencilla y rápida para determinar sí la vagina de Sceloporus torquatus tiene la capacidad de almacenar espermatozoides. En las hembras de esta especie, los espermatozoides son retenidos al menos 167 días en un sistema de túbulos especial...

  4. Co-overexpression of bcl-2 and c-myc in uterine cervix carcinomas and premalignant lesions

    Directory of Open Access Journals (Sweden)

    Z. Protrka

    2011-03-01

    Full Text Available To establish the role of co-overexpression of bcl-2 and c-myc protooncogenes in uterine cervix carcinogenesis, we examined 138 tissue samples of low grade cervical squamous intraepithelial lesions (SIL, high grade SIL, portio vaginalis uteri (PVU carcinoma in situ and PVU carcinoma invasive, stage IA-IIA (study group and 36 samples without SIL or malignancy (control group. The expression of bcl-2 and c-myc was detected immunohistochemically using a monoclonal antibody. Fisher’s exact test (P<0.05 was used to assess statistical significance. Overexpression of bcl-2 was found to increase in direct relation to the grade of the cervical lesions. High sensitivity was of great diagnostic significance for the detection of these types of changes in the uterine cervix. On the basis of high predictive values it can be said that in patients with bcl-2 overexpression there is a great possibility that they have premalignant or malignant changes in the uterine cervix. Co-overexpression of bcl-2 and c-myc oncogenes was found only in patients with PVU invasive carcinoma (6/26-23.0%. Statistically significant difference was not found in the frequency of co-overexpression in patients with PVU invasive carcinoma in relation to the control group (Fisher’s test; P=0.064. The method's sensitivity of determining these oncogenes with the aim of detecting PVU invasive carcinoma was 23%, while specificity was 72.2%. On the basis of high predictive values (100%, speaking in statistical terms, it can be concluded that all patients with co-overexpression of bcl-2 and c-myc oncogenes will have PVU invasive carcinoma. We confirmed in our research that co-overexpression of bcl-2 and c-myc oncogenes was increased only in PVU invasive carcinoma. However, a more extensive series of samples and additional tests are required to establish the prognostic significance of bcl-2 and c-myc co-overexpression in cervical carcinogenesis.

  5. Adenosquamous cell carcinoma of the cervix — clinical and prognostic characteristics of the disease

    Directory of Open Access Journals (Sweden)

    E. K. Tanriverdieva

    2012-01-01

    Full Text Available Adenosquamous cell carcinoma of the cervix is a rare form of cancer of the cervix. Because of the small number of observations adenosquamous cell carcinoma of the cervix remains poorly understood disease, although the first mention of it dates back to 1956, when A. Glucksmann, and C.D. Cherry first described of mixed carcinoma (adenoacanthoma of the uterine cervix.

  6. Congenital abnormality of the vagina complicated by haemato-pyocolpos in a 1-year labrador retriever.

    Science.gov (United States)

    Alonge, S; Romussi, S; Grieco, V; Luvoni, G C

    2015-06-01

    A 1-year-old female Labrador retriever was referred with a few days history of haematic-like vulvar discharge. Physical examination, vaginal inspection and palpation did not reveal any remarkable finding. Transabdominal ultrasound showed echogenic fluid accumulation in the vagina suggesting haemato-pyocolpos. An exploratory laparotomy was performed: a well-delimited ectasic vagina was identified. Ovariohysterectomy and partial vaginectomy and vaginoplasty were performed to spay the bitch and to remove the ectasic vagina. Post-operative recovery and 12-month follow-up were uneventful. Clinical, morphological and histological findings were consistent with a congenital abnormality of the muscular layer of the vagina complicated by haemato-pyocolpos. The disorganization of the vaginal tunica muscularis may have acted as locus minoris resistentiae in the vaginal wall. The organ was dilated and atonic due to the gradual accumulation of physiological fluids complicated by an overgrowth of genital bacteria. This congenital disorder has to be taken into account as differential diagnosis of haemato-pyocolpos with vaginal discharge in young bitches.

  7. Complete Genome Sequence of Mycoplasma bovigenitalium Strain HAZ 596 from a Bovine Vagina in Japan

    Science.gov (United States)

    Nagai, Kazuya; Murakami, Kenji

    2017-01-01

    ABSTRACT Mycoplasma bovigenitalium, a mycoplasmal species involved in various bovine diseases, including genital disease and mastitis, is also a commensal microorganism that inhabits the bovine genital organs. We present here the complete 853,553-bp genome sequence of M. bovigenitalium strain HAZ 596, which was isolated from a bovine vagina in Japan. PMID:28183755

  8. Safety, Efficiency, and Outcomes of Perineoplasty: Treatment of the Sensation of a Wide Vagina

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    Mustafa Ulubay

    2016-01-01

    Full Text Available Background. The sensation of a wide vagina is a common problem for women after childbirth. As its etiology is unknown, there is no uniform management strategy. We hypothesized that, rather than vaginal laxity, the cause was level 3 pelvic support deficiency. Methods. This retrospective study compared preoperative and postoperative genital hiatus length, perineal length, and total vaginal length in patients treated with perineoplasty for the sensation of a wide vagina. A telephone survey was used to determine postoperative patient and male partner satisfaction rates. Results. Mean age of patients was 48 (26–68 years; mean body mass index (BMI was 25.3 (17.6–33.2; and mean parity was 2.5 (2–5. Preoperative and postoperative genital hiatus, perineal length, and total vaginal length were 4.62 and 3.18 (p<0.01, 3.06 and 4.04 (p<0.01, and 9.43 and 9.43 (p=0.882, respectively. At the 6-month follow-up, the success rate of the perineoplasty procedure was 87.9%; according to a visual analog scale, partner satisfaction rate was 92.6%. Ten percent (n=4 of patients said they experienced dyspareunia during sexual intercourse at the introitus of the vagina. Conclusion. With low dyspareunia rates, low complication rates, high patient satisfaction, and satisfactory anatomical success, perineoplasty can be considered successful for treatment of the sensation of a wide vagina.

  9. Role of Visual Inspection of Cervix with Acetic Acid (VIA in Detecting Precancerous Lesions of Cervix

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    Kamrun Nessa

    2014-01-01

    Full Text Available Background: Carcinoma of cervix is the most common malignancy in female and a major public health problem worldwide. It is the leading cause of death from cancer among women in low resource settings. In Bangladesh, mortality rate is high as most of the cases with cervical cancer are diagnosed in advanced stage. World Health Organization considers cervical cancer as a preventable disease as it can be identified in preinvasive stage. Considerable efforts have been given in detection and treatment of the condition all over the world. A number of cervical cancer screening tests are available. Among them, visual inspection of cervix with acetic acid is rational and can be competently performed by physicians with proper training. Objective: To find out the feasibility of the visual inspection of cervix with acetic acid for the detection of the precancerous lesions of the cervix in our country. Materials and Methods: This cross sectional, analytical study was carried out among the patients attending the outpatient department of Bangabandhu Sheikh Mujib Medical University (BSMMU who were VIA positive and sent for colposcopy in the colposcopy clinic in the department of Obstetrics and Gynecology in BSMMU from June to December 2004. Two hundred samples were considered for this study. Results: Out of 200 cases, colposcopically 85% had CIN and invasive lesions, 4% had inflammatory lesions while 11% had normal findings. Colposcopy directed punch biopsy revealed positive lesions in 81%, 4% had inflammatory lesions while 15% had normal findings. Conclusion: The study concluded that VIA and colposcopy are the important methods in the evaluation of cervical premalignancy. VIA may be an important tool for screening of cervical cancer in low resource settings as it is simple, easy to perform and cost-effective. After screening, VIA positive cases must be referred for colposcopic evaluation. We can screen cervical cancer by VIA all over the country and thus reduce

  10. Carcinoma of the cervix with massive eosinophilia.

    Science.gov (United States)

    Lowe, D G

    1988-04-01

    Massive local eosinophilia of 100 or more eosinophils per high power field was found in 3.2% cases of invasive carcinoma of the cervix. The prevalence, length of history before presentation to surgery and histological features were similar in patients from Great Britain and Malawi, but in both populations the mean age at diagnosis was lower than in patients with cervical carcinomas without tissue eosinophilia. In some of the tumours, the malignant cells were very difficult to find because of the eosinophil infiltrate, and misinterpretation as an inflammatory lesion was possible. In the absence of circulating eosinophilia, cervical carcinomas with massive eosinophilia were found to have a better prognosis than tumours without. Five patients had circulating eosinophilia as well as local tumour eosinophil infiltration, and each of them had extensive tumour spread.

  11. Small cell carcinoma of the cervix: a case report.

    Science.gov (United States)

    Korcum, Aylin Fidan; Aksu, Gamze; Bozcuk, Hakan; Pestereli, Elif; Simsek, Tayup

    2008-04-01

    Small cell carcinoma of the uterine cervix accounts for 1-3% of all cervix cancers. It is an aggressive disease with a poor prognosis. To date, no effective treatment protocol has been determined. Surgery, radiotherapy, and chemotherapy have been used either alone or in combination. Recent data suggests that survival in patients with early staged small cell carcinoma of the cervix is better with surgery combined with chemo-radiotherapy. Here, we presented two patients with stage IB1 small cell carcinoma of the uterine cervix. For both patients, definitive surgery was performed with pelvic and para-aortic lymphadenectomy. Subsequently, they were treated with pelvic external radiotherapy and high-dose-rate intracavitary brachytherapy with concurrent cisplatin based chemotherapy. They were alive with no evidence of disease at 91 and 65 months, respectively.

  12. Epidermoid Carcinoma of the Cervix and Pregnancy. A case presentation.

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    Práxedes Rojas Quintana

    2007-12-01

    Full Text Available A 25 year-old female patient with a 12 week pregnancy was presented. She was diagnosed with an ultered Pap test. She was referred to the cervix uterine pathology specialist where an exophytic lesion compatible to cervix uterine cancer in advanced stages. The treatment as well as the conduct followed by the specialist was really hard due to the patient´s pregnant condition.

  13. Reappearance of cancer of the cervix 19 years after radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Ampil, F. [Lousiana State Univ. Medical Center, Dept. of Radiology, Louisiana (United States); Bell, M.; Martin, D. [Lousiana State Univ. Medical Center, Dept. of Obstetrics- Gynecology, Louisiana (United States)

    1997-07-01

    Most of the recurrences after Wertheim hysterectomy or definitive radiotherapy for cancer of the cervix occur within two or three years following treatment. Late recurrence is an uncommon event accounting for less than 1% of all patients with cancer of the cervix treated by radiotherapy. We present a case of reappearance of cervical cancer 19 years after irradiation and review the literature. (au) 7 refs.

  14. The localization of Toll-like receptor 2 (TLR2) in the endometrium and the cervix of dogs at different stages of the oestrous cycle and with pyometra.

    Science.gov (United States)

    Chotimanukul, S; Sirivaidyapong, S

    2012-12-01

    The aim of this study was to localize and evaluate the role of Toll-like receptor 2 (TLR2) in the endometrium and cervix of bitches at different stages of the oestrous cycle and in bitches with pyometra. Sixty-seven nulliparous dogs, ranging in age from 1 to 13 years, were allocated amongst five groups (pro-oestrus; n = 7, oestrus; n = 10, dioestrus; n = 16, anoestrus; n = 11, pyometra; n = 23). Blood samples were collected for the measurement of progesterone concentration. The mean progesterone concentration was analysed as a parameter for validating the stage of the oestrous cycle in bitches. Tissues collected from uterine horn and cervix were fixed in 4% paraformaldehyde for immunohistochemical examination of TLR2. The expression of TLR2 was assessed semi-quantitatively. No pathological changes were found in the uterine samples of healthy dogs. In bitches with pyometra, the glandular epithelium expressed TLR2 more intensely than the surface epithelium. The expression of TLR2 in the glandular epithelium was also significantly higher in healthy dogs at oestrus, dioestrus and dogs with pyometra compared with anoestrous dogs (p < 0.01). The expression of TLR2 in the stroma was not observed in the group of healthy dogs at all stages. The surface epithelium of cervix in dogs with pyometra expressed TLR2 significantly more intensely than did the stoma, whereas the expression of TLR2 during oestrus and dioestrus was absent in the stroma of cervix. This study provides the first report of immunohistochemical localization of TLR2 in the canine reproductive tract. In the present study, TLR2 was expressed in endometrial epithelium but was absent in the endometrial stroma of healthy dogs at all oestrous cycle stages. These findings suggest differential expression of TLR in endometrial cells. On the other hand, the lack of TLR2 in the stroma of healthy uteri of dogs may predispose to infection from the invading pathogens once the epithelial cells have been destroyed by the

  15. Pengaruh Senam Nifas terhadap Penurunan Tinggi Fundus Uteri pada Ibu Post Partum di RSUP DR. M. Djamil Padang

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    Nurniati Tianastia Rullynil

    2014-09-01

    Full Text Available AbstrakPerdarahan merupakan penyebab utama kesakitan dan kematian ibu pada masa nifas, dimana 50%-60% karena kegagalan miometrium berkontraksi secara sempurna. Salah satu asuhan untuk memaksimalkan kontraksi uterus pada masa nifas adalah dengan melaksanakan senam nifas, guna mempercepat proses involusi uteri. Tujuan penelitian ini adalah untuk mengetahui pengaruh senam nifas terhadap penurunan tinggi fundus uteri (TFU pada ibu post partum. Penelitian ini merupakan studi eksperimental dengan Post Test Only Control Group Design. Alat yang digunakan dalam penelitian berupa kaliper pelvimetri. Diberikan perlakuan senam nifas pada kelompok intervensi dan tidak senam nifas pada kelompok kontrol, kemudian dilakukan pengukuran tinggi fundus uteri hari ke-1, hari ke-3 dan hari ke-6. Data dianalisa menggunakan Uji General Linier Model (GLM. Rerata TFU hari ke-1 pada kelompok intervensi 12,37±0,72 dan 12,42±0,54 pada kelompok kontrol. Rerata TFU hari ke-3 pada kelompok intervensi 9,00±0,94 dan 9,87±0,75 pada kelompok kontrol. Sedangkan rerata TFU hari ke-6 pada kelompok intervensi 5,72±0,88 dan 7,37±0,68 pada kelompok kontrol. Terdapat perbedaan yang signifikan penurunan tinggi fundus uteri antara kedua kelompok pada hari ke-3 (p=0,00 dan hari ke 6 (p=0,00. Dari hasil penelitian dapat disimpulkan bahwa senam nifas berpengaruh terhadap penurunan tinggi fundus uteri. Penurunan tinggi fundus uteri pada kelompok intervensi lebih turun dibanding kelompok kontrol.Kata kunci: senam nifas, tinggi fundus uteri, post partumAbstractHemorrhage is a major cause of maternal morbidity and mortality in the puerperium, about 50%-60% of hemorrhage occurs due to failure of myometrium to contract completely. One care to maximaze uterine contraction during the puerperium is by implementing parturition gymnastics in order to accelarate the process of uterine involution. The purpose of this study was to determine the effect of parturition gymnastics on a decreasing of

  16. Foreign body in vagina: a cause of persistent vaginal discharge in children

    Directory of Open Access Journals (Sweden)

    P. Pallavee

    2013-04-01

    Full Text Available Vulvovaginitis and vaginal discharge in pediatric patients, while not uncommon, is commonly believed to be due to such causes as absence of the protective effect on the vaginal mucosa. However, other causes need also to be kept in mind. We report a case of chronic vaginal discharge in a 5 yr old, who had retained a foreign body in her vagina for 6-7 months. [Int J Reprod Contracept Obstet Gynecol 2013; 2(2.000: 224-225

  17. Bacterial flora of semen collected from Danish warmblood stallions by artificial vagina.

    Science.gov (United States)

    Madsen, M; Christensen, P

    1995-01-01

    Semen samples were collected from 21 Danish Warmblood stallions by the Colorado artificial vagina (Colorado AV, 14 samples) or by the Missouri artificial vagina (Missouri AV, 7 samples). The semen was examined bacteriologically by direct plating (DP) on blood agar plates, and by plating of semen swabs stored in Stuart's transport media (TM) at 4 degrees C for 1-4 days. No significant differences were observed between results obtained by DP and cultures of identical TM samples. Of the 21 samples examined, only 1 TM (4.8%) and 2 DP samples (9.5%) were sterile, while the rest yielded a predominantly mixed flora comprising 1 to 4 bacterial genera. The natural flora was dominated by coagulase-negative staphylococci (Staphylococcus lentus, S. capitis, S. haemolyticus, S. xylosus) (16/21 = 76%), coryneforms (11/21 = 52%) and alpha-hemolytic streptococci and lactobacilli (7/21 = 33%). Potential venereal pathogens were isolated from 7 stallions (33%). Beta-hemolytic streptococci were found in 4 stallions used for natural service, whereas Pseudomonas aeruginosa serotype 6 (2 samples) and Klebsiella pneumoniae subsp. pneumoniae capsule type K5 (1 sample) were isolated from 3 stallions used exclusively for artificial insemination. The role of the stallion as a carrier of potential venereal pathogens, and the artificial vagina as a source of contamination, is discussed in the context of mare endometritis.

  18. Compensatory structural adaptive modifications of vagina in response to functional demand in goat.

    Science.gov (United States)

    Hussin, Amer M; Zaid, Nazih W; Hussain, S O

    2014-01-01

    Vaginal biopsies and smears were collected from ten adult local healthy goats. Routine histological methods were carried out on vaginal biopsies and then stained with PAS stain. The smears were stained with Methylene blue. All samples were inspected under light microscope. The present study found that many constituents of the wall of the vagina, which have an important functional role, were absent; among these were the vaginal glands, goblet cells, muscularis mucosa, and lymphatic nodules. On the other hand, vagina showed special compensatory histological mechanisms, namely, the deep epithelial folds, the well-developed germinated stratum basale, the apparent basement membrane, and the profuse defensive cells, such as neutrophils, macrophages, lymphocytes, plasma cells, and mast cells. The general stains of this study could not recognize dendritic cells although they play an important functional role. Moreover, the herein study declared also that the vaginal smears showing many adaptive cellular mechanisms among these were, the keratinization, the process of sheet formation that lines the vaginal lumen, the process of metachromasia which is related to the cellular activity in protein synthesis, keratin, and finally the presence of endogenous microorganisms. It was concluded that all the above cellular compensatory adaptive mechanisms may compensate the lacking vaginal constituents and act to raise the immune response of the vagina.

  19. Cytokine activation during embryonic development and in hen ovary and vagina during reproductive age and Salmonella infection.

    Science.gov (United States)

    Anastasiadou, M; Michailidis, G

    2016-12-01

    Salmonellosis is one of the most important zoonotic diseases and is usually associated with consumption of Salmonella Enteritidis (SE) contaminated poultry meat or eggs. Contamination with SE is usually the result of infection of the digestive tract, or reproductive organs, especially the ovary and vagina. Thus, knowledge of endogenous innate immune mechanisms operating in the ovary and vagina of hen is an emerging aspect of reproductive physiology. Cytokines are key factors for triggering the immune response and inflammation in chicken to Salmonella infection. The aim of this study was to investigate the expression profile of 11 proinflammatory cytokines in the chicken embryos during embryonic development, as well as in the hen ovary and vagina in vivo, to investigate whether sexual maturation affects their ovarian and vaginal mRNA abundance and to determine whether cytokine expression was constitutive or induced in the ovary and vagina as a response to SE infection. RT-PCR analysis revealed that several cytokines were expressed in the chicken embryos, and in the ovary and vagina of healthy birds. Expression of various cytokines during sexual maturation appeared to be developmentally regulated. In addition, a significant up-regulation of several cytokines in the ovary and vagina of sexually mature SE infected birds compared to healthy birds of the same age was observed. These results suggest a cytokine-mediated immune response mechanism against Salmonella infection in the hen reproductive organs.

  20. Revised terminology for cervical histopathology and its implications for management of high-grade squamous intraepithelial lesions of the cervix.

    Science.gov (United States)

    Waxman, Alan G; Chelmow, David; Darragh, Teresa M; Lawson, Herschel; Moscicki, Anna-Barbara

    2012-12-01

    In March 2012, the College of American Pathologists and American Society for Colposcopy and Cervical Pathology, in collaboration with 35 stakeholder organizations, convened a consensus conference called the Lower Anogenital Squamous Terminology (LAST) Project. The recommendations of this project include using a uniform, two-tiered terminology to describe the histology of human papillomavirus-associated squamous disease across all anogenital tract tissues: vulva, vagina, cervix, penis, perianus, and anus. The recommended terminology is "low-grade" or "high-grade squamous intraepithelial lesion (SIL)." This terminology is familiar to clinicians, because it parallels the terminology of the Bethesda System cytologic reports. Biopsy results using SIL terminology may be further qualified using "intraepithelial neoplasia" (IN) terminology in parentheses. Laboratory p16 tissue immunostaining is recommended to better classify histopathology lesions that morphologically would earlier have been diagnosed as IN 2. p16 is also recommended for differentiating between high-grade squamous intraepithelial lesions and benign mimics. The LAST Project recommendations potentially affect the application of current guidelines for managing cervical squamous intraepithelial lesions. The authors offer interim guidance for managing cervical lesions diagnosed using this new terminology with special attention paid to managing young women with cervical high-grade squamous intraepithelial lesions on biopsy. Clinicians should be aware of the LAST Project recommendations, which include important changes from prior terminology.

  1. Comparative performance analysis of cervix ROI extraction and specular reflection removal algorithms for uterine cervix image analysis

    Science.gov (United States)

    Xue, Zhiyun; Antani, Sameer; Long, L. Rodney; Jeronimo, Jose; Thoma, George R.

    2007-03-01

    Cervicography is a technique for visual screening of uterine cervix images for cervical cancer. One of our research goals is the automated detection in these images of acetowhite (AW) lesions, which are sometimes correlated with cervical cancer. These lesions are characterized by the whitening of regions along the squamocolumnar junction on the cervix when treated with 5% acetic acid. Image preprocessing is required prior to invoking AW detection algorithms on cervicographic images for two reasons: (1) to remove Specular Reflections (SR) caused by camera flash, and (2) to isolate the cervix region-of-interest (ROI) from image regions that are irrelevant to the analysis. These image regions may contain medical instruments, film markup, or other non-cervix anatomy or regions, such as vaginal walls. We have qualitatively and quantitatively evaluated the performance of alternative preprocessing algorithms on a test set of 120 images. For cervix ROI detection, all approaches use a common feature set, but with varying combinations of feature weights, normalization, and clustering methods. For SR detection, while one approach uses a Gaussian Mixture Model on an intensity/saturation feature set, a second approach uses Otsu thresholding on a top-hat transformed input image. Empirical results are analyzed to derive conclusions on the performance of each approach.

  2. Mesonephric adenocarcinoma of the cervix: Case report and literature review

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    A. Dierickx

    2016-08-01

    Full Text Available A mesonephric adenocarcinoma of the cervix is a very rare tumor deriving from remnants of the mesonephric duct. Differential diagnosis from other cervical carcinomas is difficult and little is known regarding its biological behavior, prognosis, and the optimal management strategy. We present a case of a mesonephric adenocarcinoma of the cervix with a comprehensive review of the existing literature. In this case a 66-year-old woman presented with postmenopausal vaginal bleeding. She was diagnosed with a FIGO stage IIB mesonephric adenocarcinoma of the cervix and treated with neoadjuvant chemoradiotherapy and a Wertheim hysterectomy. The recovery from surgery was uneventful and the patient remains with no evidence of disease with 2 years of follow-up.

  3. Automatic detection of anatomical landmarks in uterine cervix images.

    Science.gov (United States)

    Greenspan, Hayit; Gordon, Shiri; Zimmerman, Gali; Lotenberg, Shelly; Jeronimo, Jose; Antani, Sameer; Long, Rodney

    2009-03-01

    The work focuses on a unique medical repository of digital cervicographic images ("Cervigrams") collected by the National Cancer Institute (NCI) in longitudinal multiyear studies. NCI, together with the National Library of Medicine (NLM), is developing a unique web-accessible database of the digitized cervix images to study the evolution of lesions related to cervical cancer. Tools are needed for automated analysis of the cervigram content to support cancer research. We present a multistage scheme for segmenting and labeling regions of anatomical interest within the cervigrams. In particular, we focus on the extraction of the cervix region and fine detection of the cervix boundary; specular reflection is eliminated as an important preprocessing step; in addition, the entrance to the endocervical canal (the "os"), is detected. Segmentation results are evaluated on three image sets of cervigrams that were manually labeled by NCI experts.

  4. Prevention of cancer cervix: developing a task force

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    A. Parimala

    2016-08-01

    Conclusions: A women with a preinvasive lesion of cancer cervix can be asymptomatic. This requires a task force for mass screening of all women in the community. Community health workers begin screening of all women from 21 years onwards with Pap smear and colposcopy. The trained heath workers can be used for downstaging of cancer cervix leading to a dramatic reduction in the incidence of invasive carcinoma. Even when a colposcopist detects the invasive carcinoma, it is so early that an 85-100% five-year survival rate can be achieved. [Int J Reprod Contracept Obstet Gynecol 2016; 5(8.000: 2515-2518

  5. Congenital malformation of the vaginal orifice, imperforate vagina, in the common marmoset (Callithrix jacchus).

    Science.gov (United States)

    Niimi, Kimie; Oguchi, Ayaka; Nishio, Kenji; Okano, Yasushi; Takahashi, Eiki

    2015-03-01

    The following is a report on a congenital vaginal malformation, imperforate vagina, in the common marmoset (Callithrix jacchus). This anomaly was observed for the first time in an adult female in our research colony. There was no uterine and vaginal aplasia or atresia in her grossly normal genital tract. The plasma progesterone concentration suggested that the ovarian cycle had ceased. However, this may not be related to a functional anomaly, but rather to suppressed ovulation resulting from subordination to cagemates considering the various stages of follicular development observed.

  6. Expression of LIF, VEGF,CD57 and CD68 after the transfer of rat embryos to mouse uteri

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The high failure rate of interspecific pregnancy is a major obstacle to the successful interspecific cloning of mammals. To investigate the reasons for the failure of interspecfic pregnancy between rats and mice, we transferred rat blastocysts into mouse uteri on the third day of pseudopregnancy (D3). Our previous study showed that intact rat embryos could still be observed in mouse uteri on D9. In the present study, we found that expression of CD57 and CD68 increased significantly at the maternal-fetal interface following the transfer of rat embryos. Similarly, Leukaemia inhibitory factor (LIF) expression increased, but vascular endothelial growth factor (VEGF) expession decreased. In a co-culture system, the percentage of rat ectoplacental cones (EPCs) with adhesion and outgrowth and outgrowth area on mouse uterine decidual cells were less than that of mouse EPCs. These results indicate that an increase in the immunological rejection response and a decrease in the invasiveness of rat embryos may be important reasons for the failure of interspecific pregnancy between rat and mouse.

  7. Pregnancy after radiation therapy for carcinoma of the cervix.

    Science.gov (United States)

    Browde, S; Friedman, M; Nissenbaum, M

    1986-01-01

    A successful pregnancy after intracavitary radiation therapy for carcinoma of the cervix is described. An additional 13 similar cases from the literature are reviewed. The possible reasons for the occurrence of these pregnancies despite irradiation to the ovaries, cervical canal and endometrium are discussed. The fact is emphasized that no genetic damage to the child was expected.

  8. CLINICAL ANALYSIS OF PRIMARY MALIGNANT MELANOMA OF THE CERVIX

    Institute of Scientific and Technical Information of China (English)

    Shui-qing Ma; Chun-mei Bai; Sen Zhong; Xiao-hong Yu; Jing-he Lang

    2005-01-01

    Objective To investigate the clinical and pathological characteristics of primary cervical malignant melanoma,and its prognosis.Methods The clinical and pathological data of four patients with primary malignant melanoma of the cervix were analyzed retrospectively. Nerve tissue protein S-100 and monoclonal antibody to melanoma (HMB-45) were measured in all cases by immunohistochemical method. All four patients received radical hysterectomy. Three of them received chemotherapy preoperation or postoperation, and one of them received biotherapy with interferon-γ and interleukin-2 at the same time. All the cases were followed up.Results The average age of four patients was 45 years. Clinical symptoms presented with irregular vaginal bleeding,postcoital bleeding, or increase of vaginal discharge. Gynecologic examination showed polypus papilla cauliflower-shaped or nodulated black-brown or black-blue mass on the cervix. All the four cases were pathologically diagnosed with cervical malignant melanoma. S-100 and HMB-45 were positive in all patients. Two patients died at 6 and 41 months postoperation, respectively. The other two patients survived for 3.5 and 7 years postoperation, respectively.Conclusions S-100 protein and HMB-45 play very important roles in the diagnosis of primary malignant melanoma of cervix. Radical hysterectomy, chemotherapy combined with dimethyl triazemo imidazole carboxamide and biological therapies may improve the prognosis of the primary malignant melanoma of cervix ifthe disease could be diagnosed in an early stage.

  9. Expression of c-fos and c-jun protooncogenes in the uteri of immature mice neonatally exposed to diethylstilbestrol.

    Science.gov (United States)

    Yamashita, S; Takayanagi, A; Shimizu, N

    2003-01-01

    We studied the cell-type-specific and temporal expression of c-fos and c-jun protooncogenes after 17beta-estradiol (E2) stimulation in the uteri of immature 3-week-old mice neonatally exposed to diethylstilbestrol (DES), DES-mice, and the ontogenic expression of these genes in the uteri of DES-mice using immunohistochemistry and in situ hybridization. A single E2 injection induced the transient and rapid expression of c-fos mRNA and c-Fos protein in the endometrial epithelium and endothelial cells of the blood vessels in both 3-week-old vehicle-treated controls and DES-mice; a peak of mRNA expression was 2 hours after E2 injection and that of protein expression was 2 to 3 hours after the injection. The expression of c-fos mRNA and protein after E2 stimulation was lower in the DES-mice than in the control animals. There were no significant differences in the c-jun expression patterns in both experimental groups before and after the E2 injection. The E2 injection transiently down-regulated the c-jun expression in the epithelium and up-regulated it in the stroma and myometrium. The uterine epithelium of DES-mice showed much stronger c-Jun immunostaining on days 4 and 10, compared with those of controls. Neonatal DES treatment reduced c-Jun immunoreactivity in the uterine epithelium on days 4 and 10, and increased the reaction in the stroma on day 4. These results suggested that the neonatal DES treatment induces permanent changes in the c-fos expression pattern independent of the postpuberal secretion of ovarian steroids. The changes in the expression of c-fos and c-jun protooncogenes, particularly during postnatal development, are likely to play important roles in the production of uterine abnormalities in the DES-mice.

  10. Radiation therapy for primary squamous cell carcinoma of the vagina: Stanford University experience

    Energy Technology Data Exchange (ETDEWEB)

    Spirtos, N.M.; Doshi, B.P.; Kapp, D.S.; Teng, N. (Stanford Univ. Medical Center, CA (USA))

    1989-10-01

    A retrospective analysis of 38 patients with primary squamous cell carcinoma of the vagina seen at Stanford University Medical Center from 1958 to 1984 was undertaken. Patients were analyzed with regard to symptoms, stage, treatment techniques, survival, patterns of failure, and complications. Eighteen patients were classified as FIGO Stage I, 5 as Stage II, 10 as Stage III, and 5 as Stage IV. The 5-year disease-free survival was 94% in Stage I, 80% in Stage II, 50% in Stage III, and 0% in Stage IV. Five patients (13%) had eight major complications secondary to treatment. Only 2 of 23 patients with Stage I or Stage II disease developed a recurrence. There was a significant correlation between dose and response in patients treated with radiotherapy.

  11. Struvite calculus in the vagina of a bottlenose dolphin (Tursiops truncatus).

    Science.gov (United States)

    McFee, Wayne E; Carl A, Osborne

    2004-01-01

    On 27 January 2000, a struvite calculus was observed in the vagina during necropsy of a 138-cm-long female bottlenose dolphin (Tursiops truncatus) collected from the Stono River, South Carolina (USA). Vaginal calculi have been reported in other species of cetaceans but not in bottlenose dolphins. Urinary tract infection might have been an underlying cause of the calculus. While urinary tract inflammation was not detected by light microscopic evaluation of sections of the urinary tract, it is conceivable that sufficient time had lapsed following voiding of the calculus through the urethra for urinary tract infection to have resolved. To further define the prevalence and significance of urolithiasis, prosectors of dead stranded marine mammals are encouraged to closely observe their urinary and genital tracts for calculi and to submit them for quantitative analysis.

  12. Clinical Value of Cervix Mark by Using B-mode Ultrasonography%B超宫颈评分的临床价值

    Institute of Scientific and Technical Information of China (English)

    湛秀兰; 张秀英; 常德厚; 李美光; 程东红; 陈育华; 殷惠

    1993-01-01

    1990年3月~1991年6月采用B超仪对孕期宫颈评分120例,其中50例与阴道宫颈评分对照:高分组,自然临产和100%引产成功,中分组,17.2%引产成功,82.8%用药后方可引产成功,低分组引产均失败.B超宫颈评分对引产时机、方法、剖宫产时机的选择,对早产的预防、治疗、预后有很重要的意义,本组B超宫颈评分虽与阴道宫颈评分结果相近,但B超评分有客观指标,无痛苦,无损伤,减少感染,不额外增加孕妇的经济负担,应视为妊娠期宫颈评分的首选方法.%0ne hundred and twenty cases of cervices which were in gestation were marked by US in our hospital from march,1990 to June,1991.Fifty if then were compared with vagina cervix mark.The high-grade group:Natural parturition or 100%were induced successfully.The middle-grade group:17.2%were induced successfully.82.8%were induced unsuccessfully.Using US to mark cervices is very useful to the choice of opportunity of induction opportunity and its method,the deciston casearean birth,as well as for the prevention,treatment and prognosis of prevature delivery.Although the reSUIts of this group of US cervix mark is similar to that of vagina cerovix mark,US mark has objective indexes,no pain and no harm.It can reduce infection and does not increase the economic bruden of pregnant woman.It should be choiced first as the method to mark the gastationing cervices.

  13. Genome Sequences of 15 Gardnerella vaginalis Strains Isolated from the Vaginas of Women with and without Bacterial Vaginosis

    Science.gov (United States)

    Robinson, Lloyd S.; Perry, Justin; Lek, Sai; Wollam, Aye; Sodergren, Erica; Weinstock, George

    2016-01-01

    Gardnerella vaginalis is a predominant species in bacterial vaginosis, a dysbiosis of the vagina that is associated with adverse health outcomes, including preterm birth. Here, we present the draft genome sequences of 15 Gardnerella vaginalis strains (now available through BEI Resources) isolated from women with and without bacterial vaginosis. PMID:27688326

  14. Cloprostenol treatment of feline open-cervix pyometra.

    Science.gov (United States)

    García Mitacek, María C; Stornelli, María C; Tittarelli, Claudia M; Nuñez Favre, Romina; de la Sota, Rodolfo L; Stornelli, María A

    2014-02-01

    Treatment with cloprostenol, a prostaglandin synthetic analogue, was evaluated in five queens with open-cervix pyometra. Cloprostenol was administered (5 μg/kg body weight SC) on 3 consecutive days and amoxicillin (20 mg/kg body weight IM) on 7 consecutive days. Transient post-injection reactions caused by cloprostenol administration included diarrhea, vomiting and vocalizations. Reactions began as quickly as 10 mins after cloprostenol administration and lasted as long as 30 mins. All queens improved clinically after cloprostenol treatment and remained healthy until the end of the study, 1 year after treatment. All queens resumed normal estrous cycles without further treatment and two (40%) delivered a normal litter. In conclusion, use of cloprostenol is an acceptable treatment for open-cervix pyometra in queens.

  15. Sacral plexus injury after radiotherapy for carcinoma of cervix

    Energy Technology Data Exchange (ETDEWEB)

    Stryker, J.A.; Sommerville, K.; Perez, R.; Velkley, D.E. (Pennsylvania State Univ., Hershey (USA))

    1990-10-01

    A 42-year-old woman developed lower extremity weakness and sensory loss 1 year after external and intracavitary radiotherapy for Stage IB carcinoma of cervix. She has been followed for 5 years posttreatment, and the neurologic abnormalities have persisted, but no evidence of recurrent carcinoma has been found. We believe this to be a rare case of sacral plexus radiculopathy developing as a late complication after radiotherapy. Suggestions are made for improving the radiotherapy technique to prevent this complication in future cases.

  16. Lymphoma of uterine cervix: magnetic resonance imaging findings

    Energy Technology Data Exchange (ETDEWEB)

    Kanaan, Daniel; Constantino, Carolina Pesce Lamas; Souza, Rodrigo Canellas de, E-mail: daniel.kanaan@hotmail.com [Department of Radiology, Universidade Federal do Rio de Janeiro (UFRJ), RJ (Brazil); Parente, Daniella Braz [Instituto D' Or de Pesquisa e Ensino, Rio de Janeiro, RJ (Brazil)

    2012-05-15

    Lymphoma of the cervix is a rare disease. About 1.0% to 1.5% of extranodal lymphomas originates in the female genital tract. The clinical presentation of this condition is nonspecific and magnetic resonance imaging is important for diagnostic elucidation. The present report describes the case of a 80-year-old patient with lumbar pain, whose magnetic resonance imaging showed a large uterine mass. The final diagnosis was lymphoma. (author)

  17. Epidemiological and clinical correlates of chlamydial infection of the cervix.

    OpenAIRE

    Arya, O P; Mallinson, H; Goddard, A D

    1981-01-01

    Of 474 women studied to identify epidemiological and clinical correlates of chlamydial infection of the cervix, Chlamydia trachomatis was isolated from 158 (33.3%) of all women, from 48.3% of those infected with Neisseria gonorrhoeae, from 43% of the sexual consorts of men with nongonococcal urethritis, and from 74% of those whose consorts were also infected with C trachomatis. C trachomatis was the sole pathogen found in 58 women. Age, marital state, occupation, past history of gonorrhoea, m...

  18. Do All Cases of Diagnosed Carcinoma Cervix Need HIV Screening?

    Directory of Open Access Journals (Sweden)

    Anil Khurana

    2014-05-01

    Full Text Available Background: This observational study was aimed to determine the frequency of existence of sero-positivity for human immunodeficiency virus (HIV infection among women of age between 25 to 75 years with invasive cervical carcinoma and to decide whether HIV testing should be included as part of the initial routine work-up of cervical cancer patients. Methods: Histologically proven 120 cases of invasive carcinoma cervix, who came for treatment between 2009-2013, in the department of radiotherapy, after counselling gave consent were investigated for HIV by immunochromatography based rapid test. Results: Out of 120 patients investigated, reports revealed that only two patients (1.67% were HIV seropositive. Both of these patients were already HIV seropositive and on gynaecological screening were found to have malignancy of cervix. No patient of diagnosed carcinoma cervix was found seropositive for HIV. Most patients (106/120 were above 40 years of age, from rural background (92/120 and housewives (80/120. Only 15% (18/120 were smokers. 95.8% (115/120 were of stage II and III. None presented with metastasis. Most common pathology was moderately differentiated carcinoma, in 76 patients (63.3%. Conclusion: Screening for HIV, as part of the initial work up for cervical cancer is not necessary in countries with limited resources and low HIV prevalence.

  19. Pulmonary lymphangitic carcinomatosis from squamous cell carcinoma of the cervix

    Directory of Open Access Journals (Sweden)

    Senger Jenna-Lynn B

    2010-12-01

    Full Text Available Abstract Introduction Pulmonary metastasis presenting as lymphangitic carcinomatosis arising from squamous cell carcinoma (SCC of the cervix is a rare event. Poorly represented in the literature, this event is associated with a difficulty in accurate diagnosis, b grave prognosis, and the c lack of recognized predisposing risk factors. Case Report A 50 year-old female presented at our practice with a three-month history of a productive cough associated with dyspnoea and shortness of breath. A chest x-ray and computed tomography (CT scan revealed multiple bilateral patchy areas with subsegmental atelectasis in both lungs which was investigated with a bronchoscopy, left thoracoscopy, and a left lung biopsy. Pathological examination of the wedge biopsy of the left upper lobe revealed neoplastic sheets of cell disturbed along the septal vessels, perivascular/peribronchial lymphatics, and the subpleural lymphatics. This lymphangitic carcinomatosis was confirmed to be metastatic from SCC of the cervix that had been diagnosed and treated two years ago. She was treated with systemic Carbo/Taxol chemotherapy and corticosteroids as a palliative measure. Despite temporary improvement, she died 13 months later. Conclusion Pulmonary lymphangitic carcinomatosis is a rare manifestation of metastatic SCC of the cervix. As clinical presentations including radiographic imaging mimics other pulmonary entities, accurate diagnosis remains a challenge. Increased clinical awareness of such patterns of metastases in cervical cancer supported by accurate pathological diagnosis is imperative to guide appropriate therapy in these patients.

  20. Management of Adenocarcinoma In Situ of Cervix in Pregnancy

    Directory of Open Access Journals (Sweden)

    Alireza Abidi

    2008-03-01

    Full Text Available Adenocarcinoma in situ is one of the premalignant lesions of the cervix and its incidence is believed to be increasing while the pathogenesis of the disease is not clearly understood. Management of Adenocarcinoma in situ (AIS unlike carcinoma in situ (CIS has not been clearly described in the current literature. Here we describe conservative management and serial colposcopy of two pregnant women with adenocarcinoma in situ of the cervix. Both of the cases were diagnosed initially with abnormal Pap smears and were confirmed by colposcopic directed biopsy. None of the patients agreed with any invasive procedure during pregnancy and both of them were followed with serial colposcopy. None of the lesions showed any evidence of progression. All cases underwent cold knife cone biopsies in their postpartum period. Hysterectomy as the final treatment has been done in both cases with no evidence of progression of the disease during pregnancy. We concluded that adenocarcinoma in situ of the cervix during pregnancy could be managed conservatively with definite treatment postponed till after delivery.

  1. Verhalten klinisch-chemischer und hormonanalytischer Parameter bei Kühen mit und nach einem Prolapsus uteri : Versuch einer Kausalitätsklärung

    OpenAIRE

    Seitz, Klaus

    2008-01-01

    Das Ziel der vorgelegten Arbeit bestand zum einen darin, anhand der Bestimmung klinisch–chemischer und hormonanalytischer Parameter von Rindern, welche an einem Prolapsus uteri erkrankt waren sowie von gesunden Kontrolltieren im gleichen postpartalen Status, Hinweise über die Kausalität dieser Erkrankung zu erlangen. Zum anderen war zu eruieren, ob im Verlauf des Frühpuerperiums bei den betroffenen Tieren anhaltende Imbalanzen bestimmter Stoffwechselparameter bestehen. Weiterhin sollte eine M...

  2. Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains

    Directory of Open Access Journals (Sweden)

    Juliana M. A. Agostinho

    2014-01-01

    Full Text Available Pyometra is recognized as one of the main causes of disease and death in the bitch, and Escherichia coli is the major pathogen associated with this disease. In this study, 70 E. coli isolates from the uteri horn, mouth, and rectum of bitches suffering from the disease and 43 E. coli isolates from the rectum of clinically healthy bitches were examined for the presence of uropathogenic virulence genes and susceptibility to antimicrobial drugs. DNA profiles of isolates from uteri horn and mouth in bitches with pyometra were compared by REP, ERIC, and BOX-PCR. Virulence gene frequencies detected in isolates from canine pyometra were as follows: 95.7% fim, 27.1% iss, 25.7% hly, 18.5% iuc, and 17.1% usp. Predominant resistance was determined for cephalothin, ampicillin, and nalidixic acid among the isolates from all sites examined. Multidrug resistance was found on ∼50% pyometra isolates. Using the genotypic methods some isolates from uteri, pus, and saliva of the same bitch proved to have identical DNA profiles which is a reason for concern due to the close relationship between household pets and humans.

  3. Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains.

    Science.gov (United States)

    Agostinho, Juliana M A; de Souza, Andressa; Schocken-Iturrino, Ruben P; Beraldo, Lívia G; Borges, Clarissa A; Avila, Fernando A; Marin, José M

    2014-01-01

    Pyometra is recognized as one of the main causes of disease and death in the bitch, and Escherichia coli is the major pathogen associated with this disease. In this study, 70 E. coli isolates from the uteri horn, mouth, and rectum of bitches suffering from the disease and 43 E. coli isolates from the rectum of clinically healthy bitches were examined for the presence of uropathogenic virulence genes and susceptibility to antimicrobial drugs. DNA profiles of isolates from uteri horn and mouth in bitches with pyometra were compared by REP, ERIC, and BOX-PCR. Virulence gene frequencies detected in isolates from canine pyometra were as follows: 95.7% fim, 27.1% iss, 25.7% hly, 18.5% iuc, and 17.1% usp. Predominant resistance was determined for cephalothin, ampicillin, and nalidixic acid among the isolates from all sites examined. Multidrug resistance was found on ∼ 50% pyometra isolates. Using the genotypic methods some isolates from uteri, pus, and saliva of the same bitch proved to have identical DNA profiles which is a reason for concern due to the close relationship between household pets and humans.

  4. Development of a new transcervical artificial insemination method for sheep: effects of a new transcervical artificial insemination catheter and traversing the cervix on semen quality and fertility.

    Science.gov (United States)

    Wulster-Radcliffe, Meghan C; Lewis, Gregory S

    2002-10-15

    The difficulty of traversing the cervix severely limits transcervical artificial insemination (TC AI) in sheep. Cervical trauma and poorly designed instruments can reduce fertility after AI. To overcome problems associated with TC AI, we developed a new TC AI catheter. Three bench experiments were conducted to determine the effects of the new TC AI catheter on semen quality independent of the effects of moving the catheter through the cervix. In each of the three bench experiments, the standard laparoscopic instrument for intrauterine AI in sheep was used as the control for the TC AI catheter. In Experiment 1, the total volume of semen extender expelled and void volumes for both types of AI instrument (TC versus laparoscopic) were determined. In Experiment 2, the effects of each type of AI instrument (TC versus laparoscopic) on semen quality, estimated as percentage motility and percentage forward progressive motility, of frozen-thawed semen was determined. In Experiment 3, the effects of both types of AI instrument (TC versus laparoscopic) on number of spermatozoa expelled was determined. The type of AI instrument affected neither semen quality nor the number of spermatozoa expelled. However, void volume differed (P synchronize estrus, progestogenated pessaries were inserted and left in place for 12 days. On Day 5 after pessary insertion, PGF2alpha (15 mg) was given i.m. At pessary removal, 400 IU of eCG were administered i.m. Ewes were inseminated 48-52 h after pessary removal using fresh diluted semen (200 x 10(6) to 350 x 10(6) spermatozoa per 0.2 ml) pooled from the same four rams each day during the experiment. At 72 h after AI, uteri were collected postmortem and flushed. Oocytes and embryos were recovered and evaluated. Treatments did not affect (P > 0.01) ovum and embryo recovery rate (mean = 87.3%), fertilization rate (59.3%), or Day 3 pregnancy rate (mean = 66.6%). We conclude from these data that the use of our new TC AI catheter for TC AI or

  5. Recurrent paediatric pinworm infection of the vagina as a potential reservoir for Enterobius vermicularis.

    Science.gov (United States)

    Kashyap, B; Samantray, J C; Kumar, S; Jhamb, R; Singh, A K; Kaur, I R

    2014-09-01

    Enterobius vermicularis infection remains one of the most common parasitic infections, particularly prevalent in children. Enterobiasis, although not usually dangerous, may cause significant morbidity. Elimination of the parasite from a family or an institution often poses problems, either because of an incomplete cure or re-infection. While there have been limited reports of ectopic enterobiasis throughout the world, ours is probably one of the rarest reports of recurrent vaginal E. vermicularis infection in the absence of any gastrointestinal symptoms despite complete treatment. A 4-year-old girl presented with recurrent episodes of vulval itching on 3-4 occasions over 2 years. There was no pruritis ani nor urinary/gastrointestinal complaints. The vulva was inflamed with 4-5 living worms, 6-7 mm in length, emerging from the anterior vaginal fornix, but with no vaginal discharge. Direct microscopic examination of vaginal swabs revealed adult worms of Enterobius but no eggs. Repeated stool samples from the patient, parents and a sibling were negative. The patient was treated orally with 100 mg of mebendazole for 3 days followed by two more courses at 3-week intervals over a period of 3 months. Recurrent vaginal enterobiasis despite complete treatment and in the absence of any gastrointestinal involvement suggests that the vagina is a potential reservoir for E. vermicularis, which supports the theory of rare ectopic enterobiasis through the ascending pathway of the female genital tract.

  6. Paraganglioma of the vagina: a case report and review of the literature

    Directory of Open Access Journals (Sweden)

    Cai T

    2014-06-01

    Full Text Available Tao Cai,1 Yucong Li,2 Qingming Jiang,3 Dong Wang,2 Yu Huang2 1Department of Dermatology, The First Affiliated Hospital of Chongqing Medical University, 2Department of Gynecologic Oncology, 3Department of Pathology, Chongqing Cancer Research Institute, Chongqing, People’s Republic of China Abstract: Vaginal paraganglioma is a rare and unusual tumor occurring in the vaginal wall. It is a solitary primary paraganglioma, especially in atypical sites. Herein, we report an unusual case of a 17-year-old woman who had not experienced vomiting, or hypertension. She was found to have an immobile solid mass in the right side of her vaginal wall. Positron emission tomography/computed tomography scans revealed a well-defined solid ovoid mass adjacent to the bladder and pelvic floor. Tumor markers were within the normal range. A transient blood pressure increase occurred during the biopsy. After oral administration of antihypertensive drugs, surgery was performed to completely remove the mass. Histopathological examination indicated that it was a paraganglioma of the vagina. Repeat computed tomography examination did not reveal any local recurrence or distant metastasis during the 12-month follow-up period. Keywords: vaginal paraganglioma, neuroendocrine tumor, clinical features, treatment

  7. Sugar expression in the mucosae of the canine uterus and vagina during the oestrous cycle and with pyometra.

    Science.gov (United States)

    Yasunaga, Y; Takeuchi, T; Shimokawa, T; Asano, A; Nabeta, M; Ohta, Y

    2013-04-01

    The pathogenesis of canine pyometra is still unclear, but bacterial infection of the endometrium, mediated by bacterial lectins, is suspected to induce pyometra. The aim of this study was to investigate sugar expression in the mucosae of the uterus and vagina of healthy dogs with normal oestrous cycles and in dogs with pyometra, using a panel of lectins to investigate the pathogenesis of pyometra. In dogs with pyometra, the uterine and vaginal mucosae were positive for lectins that selectively bind to glucose or mannose, especially during days 7-10 and 30-40 of dioestrus. These results suggest that temporal changes in sugar expression in the uterus and vagina present an opportunity for pathogens to infect the endometrium, causing pyometra.

  8. MRI findings of small cell neuroendocrime carcinoma of the uterine cervix: A case report

    Energy Technology Data Exchange (ETDEWEB)

    Won, You Sun; Yi, Boem Ha; Lee, Hae Kyung; Lee, Min Hee; Choi, Seo Youn; Kwak, Jeong Ja [Soonchunhyang University College of Medicine, Bucheon Hospital, Bucheon (Korea, Republic of)

    2015-10-15

    Small cell neuroendocrine carcinoma of the uterine cervix is a rare primary neoplasm, accounting for less than 5% of all uterine cervical cancers. The tumor is known to have an aggressive behavior and poor prognosis. In this article, we present the MRI findings of 5 cases of pathologically-proven small cell neuroendocrine carcinoma of the uterine cervix, including diffusion-weighted images.

  9. [Case report of live threatening complications due to self insertion of foreign body into the vagina for masturbation purpose].

    Science.gov (United States)

    Kuzaka, Bolesław; Kobryń, Andrzej; Niemierko, Maciej; Czaplicki, Maciej

    2009-01-01

    Authors have reported a case report of life threatening complications due to insertion of foreign bodies into the vagina, because of masturbation purpose. In this case subsequently came to perforation of the urinary bladder by the huge calculus that developed over the foreign body, and next to the peritoneum with development peritonitis and acute renal insufficiency with the need of dialysotherapy. After a number of surgical operations, the patient with a definitive percutaneous nephrostomy was discharged.

  10. A RARE C.T. SCAN FINDING OF FOREIGN BODY VAGINA, A HARD FRUIT, KEPT AS PESSARY FOR UTERINE PROLAPSE

    Directory of Open Access Journals (Sweden)

    Radha Rani

    2014-06-01

    Full Text Available Vaginal pessaries still have a role in the management of uterine prolapse, particularly in elderly patients. The presence of a foreign body or device in the female pelvis can be a potential source of confusion to the radiologists, particularly to the inexperienced reader. We present a rare C.T. scan finding of a hard fruit kept in vagina 5yrs back for uterine prolapse.

  11. Topometric preparation and interstitial gamma-therapy of patients with recurrencies and metastases of uterine cancer to the vagina

    Energy Technology Data Exchange (ETDEWEB)

    Bal' ter, S.A.; Androsov, N.S.; Strel' nitskaya, T.A.; Shpikalov, V.L. (Akademiya Meditsinskikh Nauk SSSR, Moscow. Onkologicheskij Nauchnyj Tsentr)

    1984-08-01

    The developed technique of preradiation topometry in patients with recurrencies and metastases of uterine cancer to the vagina allows to visualize boundaries of soft-tissue tumor formation and to specify its localization, sizes and relation with urethra, rectum, urinary bladder. Results of topometric investigation are used for individual planning of interstitial radiotherapy: fabrication of fixing instruments, choice of a number of sources, their activity, position, and exposure duration.

  12. 中青年宫颈癌患者拒绝放射治疗原因的调查分析%Investigation and Analysis of Reasons that Patients with Carcinoma of Cervix Uteri Refused Radiotherapy

    Institute of Scientific and Technical Information of China (English)

    王玉龙; 刘智; 刘立新; 贺培娃; 刘彩芳; 冯炎

    2003-01-01

    目的:通过对宫颈癌患者拒绝放射治疗原因的调查分析,采取一系列应对策略,提高治疗效果.方法:对 26 例宫颈癌患者拒绝放射治疗的原因调查,进行多因素相关统计分析,针对原因采取应对策略缓解心理应激.应对策略包括:转变放疗医生的观念、制定科普宣教方案、加强疏导、介绍有关放疗的知识、努力为患者营造一个充满亲情的社会环境,进行因人而异的心理干预.结果:26 例拒绝放疗的患者中,23 例患者接受了放射治疗,有效率达 88.45%.结论:大部分最初拒绝放射治疗的中青年宫颈癌患者,在放疗医生的耐心讲解和科普宣教下能够消除心理障碍,积极配合放射治疗.

  13. Villoglandular papillary adenocarcinoma of the uterine cervix with immunohistochemical characteristics.

    Science.gov (United States)

    Polat, A; Düsmez, D; Pata, O; Aydin, O; Egilmez, R

    2002-09-01

    Villoglandular papillary adenocarcinoma of uterine cervix has been recently described and to date fewer than a hundred cases have been reported in the world literature. Here we present a 38-year-old woman who underwent radical hysterectomy combined bilateral pelvic lymphoadenectomy and after 28 months postoperatively no lymph node metastasis and no evidence of recurrent disease ocurred. Immunohistochemically Ki-67 overexpression was detected in the tumour, with no immunoreactivity with p53, estrogen and progesteron receptors and broadly-reactive human papilloma virus including types 6, 11, 16, 18, 31, 33, 42, 51, 52, 56, and 58. In this paper, clinical, macroscopical, microscopical and immunohistochemical characteristics of this tumour are reviewed.

  14. Extra-amniotic prostaglandin E2 and the unfavourable cervix.

    Science.gov (United States)

    Shepherd, J; Sims, C; Craft, I

    1976-10-02

    A small dose of prostaglandin E2 suspended in a viscous medium was instilled as a single application into the extra-amniotic space of patients with unfavourable induction features the day before planned induction in an attempt to improve the condition of the cervix. Two groups of 15 patients were studied, one receiving prostaglandin E2 250 mug suspended in methyl ethyl cellulose ('Tylose') 6% solution, and the other tylose alone. Cervical status did not change in those receiving tylose alone, whereas a significant improvement occurred in 14 out of 15 patients receiving the prostaglandin. Labour began before formal induction in 1 patient receiving tylose and in 8 receiving prostaglandin.

  15. Antagonistic Potential against Pathogenic Microorganisms and Hydrogen Peroxide Production of Indigenous Lactobacilli Isolated from Vagina of Chinese Pregnant Women

    Institute of Scientific and Technical Information of China (English)

    HENG-YI XU; WAN-HONG TIAN; CUI-XIANG WAN; LI-JUN JIA; LAN-YIN WANG; JING YUAN; CHUN-MEI LIU; MING ZENG; HUA WEI

    2008-01-01

    Objective To investigate the indigenous lactobacilli from the vagina of pregnant women and to screen the isolates with antagonistic potential against pathogenic microorganisms. Methods The strains were isolated from pregnant women's vagina and identified using the APISOCH system. The ability of the isolates to produce hydrogen peroxide was analyzed semi-quantitatively using the TMB-HRP-MRS agar. The antagonistic effects of the isolates on pathogenic microorganisms were determined with a double layer agar plate. Results One hundred and three lactobacilli strains were isolated from 60 samples of vaginal secretion from healthy pregnant women. Among them, 78 strains could produce hydrogen peroxide, in which 68%, 80%, 80%, and 88% had antagonistic effects against Candida albicans CMCC98001, Staphylococcus aureus CMCC26003, Escherichia coli CMCC44113, and Pseudomonas aeruginosa CMCC10110, respectively. Conclusion The recovery of hydrogen peroxide-producing lactobacilli decreases with the increasing pregnant age and time. The most commonly isolated species from vagina of Chinese pregnant women are Lactobacillus acidophilus and Lactobacillus crispatus. Most of L. Acidophilus and L. Crispatus produce a high H2O2 level.

  16. In Utero Exposure to Low Doses of Bisphenol A Lead to Long-term Deleterious Effects in the Vagina

    Directory of Open Access Journals (Sweden)

    G. Schönfelder

    2002-01-01

    Full Text Available The origins of the “endocrine disrupter hypothesis” may be traced to reports on adolescent daughters born to women who had taken the highly potent synthetic estrogen, diethylstilbestrol, while pregnant, and who developed a rare form of vaginal cancer and adenocarcinoma. Bisphenol A (BPA is an estrogenic chemical that is highly employed in the manufacture of a wide range of consumer products. Some observational studies have suggested that the amounts of BPA to which we are exposed could alter the reproductive organs of developing rodents. We examined the influence of BPA at low doses to address the questions of (a whether in utero exposure affects the vagina of the offspring and (b which mechanisms cause the toxic effects. Gravid Sprague-Dawley dams were administered either 0.1 (low dose or 50 mg/kg per day BPA, the no observed effect level, or 0.2 mg/kg per day 17αethinyl estradiol by gavage. Striking morphological changes were observed in the vagina of postpubertal offspring leading us to examine vaginal estrogen receptor (ER expression because BPA binds to the ERα, which is important for growth of the vaginal epithelium. We show that the full-length ERα is not expressed during estrus in the vagina of female offspring exposed to either dose of BPA when compared to the control group, whereas ERα expression does not differ from the control group during the diestrus stage. ERa downregulation seems to be responsible for the observed altered vaginal morphology.

  17. Smooth muscle pharmacology in the isolated virgin and pregnant rat uterus and cervix.

    Science.gov (United States)

    Darios, Emma S; Seitz, Bridget; Watts, Stephanie W

    2012-06-01

    Uterine smooth muscle function is established, but comparatively little is known about cervical smooth muscle pharmacology. We performed a proof-of-principle experiment that smooth muscle was expressed in the cervix in both virgin and pregnant rats, using the uterus as a comparator. We tested whether all tissues were pharmacologically responsive to contractile and relaxant agonists. Immunohistochemistry revealed the expression of smooth muscle α-actin in all tissues. The isolated tissue bath was used to measure isometric contractility of uterine strips and whole cervices from virgin and pregnant (day 11 ± 2) female Sprague-Dawley rats. We tested classic activators of uterine smooth muscle contraction and relaxation in both uterus and cervix. All tissues contracted to the depolarizing agent potassium chloride, prostaglandin F2α, muscarinic cholinergic agonist carbachol [2-[(aminocarbonxyl)oxy]-N,N,N-trimethylethanaminium chloride], and 5-hydroxytryptamine. Unlike other tissues, the pregnant cervix did not contract to oxytocin, but the oxytocin receptor was present. Both cervix and uterus (virgin and pregnant) had concentration-dependent, near-complete relaxation to the adrenergic agonist norepinephrine and adenylate cyclase activator forskolin [(3R,4aR,5S,6S,6aS,10S,10aR,10bS)-6,10-10b-trihydroxy-3,4a,7,10a-pentamethyl-1-oxo-3-vinyldodecahydro-1H-benzo[f] chroment-5-yl acetate]. The β-adrenergic receptor agonist isoproterenol was less potent in pregnant cervix versus virgin by ∼10-fold. All tissues, particularly the cervix, responded poorly to the nitric-oxide donor sodium nitroprusside, relaxing ∼20% maximally. These findings support the importance of smooth muscle in the cervix, the use of the isolated cervix in pharmacological studies, and a similarity between smooth muscle pharmacology of the nonpregnant uterus and cervix. This work highlights the unappreciated smooth muscle function of the cervix versus uterus and cervical changes in pharmacology during

  18. Mesonephric adenocarcinoma of the vagina. Diagnosis and multimodal treatment of a rare tumor and analysis of worldwide experience

    Energy Technology Data Exchange (ETDEWEB)

    Mueller, Iris; Jacobs, Volker R.; Bogner, Gerhard; Staudach, Alfons; Koch, Horst; Wolfrum-Ristau, Pia; Schausberger, Christiane; Fischer, Thorsten [Paracelsus Medical University (PMU), Department of Obstetrics and Gynecology (OB/GYN), Salzburg (Austria); Kametriser, Gerhard; Sedlmayer, Felix [Paracelsus Medical University (PMU), Department of Radiology and Radiation Oncology, 5020 Salzburg (Austria)

    2016-09-15

    Mesonephric adenocarcinoma of the vagina is an extremely rare tumor of the female genital tract, with only a few cases reported so far worldwide. Consequently, there is no established standard treatment and limited knowledge about the prognosis and biologic behavior of vaginal mesonephric adenocarcinoma. This report documents a new case of vaginal mesonephric adenocarcinoma diagnosed in a 54-year-old woman, and analyzes this in the context of all previously published cases. MRI demonstrated that the 2.5 x 1.8 cm tumor of the vaginal wall was invading urethra and bladder. Following surgical excision, histologic analysis determined mesonephric adenocarcinoma of the vagina, stage pT2 R1. In order to avoid the mutilating extended surgery which would be required to reach R0 and considerable impairment of quality of life, adjuvant radiochemotherapy was administered with external radiation and brachytherapy, including 5 cycles of cisplatin (40 mg/m{sup 2}) for radiosensitization. After 4 years of continuous oncologic follow-up, the patient is alive and clinically free of disease. In this case it was shown that adjuvant radiochemotherapy with radiation and brachytherapy was effective to manage the surgical R1 situation and maintain the patient's life quality. More published cases reports are needed to gradually substantiate optimal treatment strategies. (orig.) [German] Das mesonephrische Adenokarzinom der Vagina ist ein aeusserst seltener Tumor des weiblichen Genitaltrakts. In der internationalen Literatur finden sich nur wenig gut dokumentierte Faelle. Das biologische Verhaltensmuster dieses Tumors und dessen Prognose sind weitgehend unbekannt. In diesem Bericht wird ein neuer Fall einer 54-jaehrigen Frau mit einem mesonephrischen Adenokarzinom der Vagina vorgestellt und unter Beruecksichtigung aller bisher publizierten Faelle analysiert. Bei dem 2,5 x 1,8 cm grossen Tumor der Vaginalwand zeigte sich in der Magnetresonanztomographie eine Infiltration der Urethra und

  19. The Lactobacillus flora in vagina and rectum of fertile and postmenopausal healthy Swedish women

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    Stjernquist Martin

    2011-05-01

    Full Text Available Abstract Background Lactobacillus species are the most often found inhabitants of vaginal ecosystem of fertile women. In postmenopausal women with low oestrogen levels, Lactobacillus flora is diminishing or absent. However, no studies have been performed to investigate the correlation between oestrogen levels and the lactobacilli in the gut. The aim of the present study was to investigate the relation in healthy women between vaginal and rectal microbial flora as well as possible variations with hormone levels. Methods Vaginal and rectal smears were taken from 20 healthy fertile women, average 40 years (range 28-49 years, in two different phases of the menstrual cycle, and from 20 postmenopausal women, average 60 years (range 52-85 years. Serum sex hormone levels were analyzed. Bacteria from the smears isolated on Rogosa Agar were grouped by Randomly Amplified Polymorphic DNA and identified by multiplex PCR and partial 16S rRNA gene sequencing. Results Lactobacillus crispatus was more often found in the vaginal flora of fertile women than in that of postmenopausal (p = 0.036. Fifteen of 20 fertile women had lactobacilli in their rectal smears compared to 10 postmenopausal women (p = 0.071. There was no correlation between the number of bacteria in vagina and rectum, or between the number of bacteria and hormonal levels. Neither could any association between the presence of rectal lactobacilli and hormonal levels be found. Conclusion Lactobacillus crispatus was more prevalent in the vaginal flora of fertile women, whereas the Lactobacillus flora of rectum did not correlate to the vaginal flora nor to hormonal levels.

  20. Clinical holistic medicine: holistic sexology and acupressure through the vagina (Hippocratic pelvic massage).

    Science.gov (United States)

    Ventegodt, Søren; Clausen, Birgitte; Omar, Hatim A; Merrick, Joav

    2006-03-07

    Many gynecological and sexological problems (like urine incontinence, chronic pelvic pains, vulvodynia, and lack of lust, excitement, and orgasm) are resistant to standard medical treatment. In our work at the Research Clinic for Holistic Medicine in Copenhagen, we have found that vaginal acupressure, or Hippocratic pelvic massage, can help some of these problems. Technically, it is a very simple procedure as it corresponds to the explorative phase of the standard pelvic examination, supplemented with the patient's report on the feelings it provokes and the processing and integration of these feelings. Sometimes it can be very difficult to control the emotions released by the technique, i.e., regression to earlier traumas from childhood sexual abuse. This review discusses the theory behind vaginal acupressure, ethical aspects, and presentation of a case story. This procedure helped the patient to become present in her pelvis and to integrate old traumas with painful emotions. Holistic gynecology and sexology can help the patient to identify and let go of negative feelings, beliefs, and attitudes related to sex, gender, sexual organs, body, and soul at large. Shame, guilt, helplessness, fear, disgust, anxiety, anger, hatred, and other strong feelings are almost always an important part of a sexual or functional problem as these feelings are "held" by the tissue of the pelvis and sexual organs. Acupressure through the vagina/pelvic massage must be done with great care by an experienced physician, with a third person present, after obtaining consent and the necessary trust of the patient. It must be followed by conversational therapy and further holistic existential processing.

  1. Clinical Holistic Medicine: Holistic Sexology and Acupressure Through the Vagina (Hippocratic Pelvic Massage

    Directory of Open Access Journals (Sweden)

    Søren Ventegodt

    2006-01-01

    Full Text Available Many gynecological and sexological problems (like urine incontinence, chronic pelvic pains, vulvodynia, and lack of lust, excitement, and orgasm are resistant to standard medical treatment. In our work at the Research Clinic for Holistic Medicine in Copenhagen, we have found that vaginal acupressure, or Hippocratic pelvic massage, can help some of these problems. Technically, it is a very simple procedure as it corresponds to the explorative phase of the standard pelvic examination, supplemented with the patient's report on the feelings it provokes and the processing and integration of these feelings. Sometimes it can be very difficult to control the emotions released by the technique, i.e., regression to earlier traumas from childhood sexual abuse. This review discusses the theory behind vaginal acupressure, ethical aspects, and presentation of a case story. This procedure helped the patient to become present in her pelvis and to integrate old traumas with painful emotions. Holistic gynecology and sexology can help the patient to identify and let go of negative feelings, beliefs, and attitudes related to sex, gender, sexual organs, body, and soul at large. Shame, guilt, helplessness, fear, disgust, anxiety, anger, hatred, and other strong feelings are almost always an important part of a sexual or functional problem as these feelings are “held” by the tissue of the pelvis and sexual organs. Acupressure through the vagina/pelvic massage must be done with great care by an experienced physician, with a third person present, after obtaining consent and the necessary trust of the patient. It must be followed by conversational therapy and further holistic existential processing.

  2. Seminal plasma protein profiles of ejaculates obtained by internal artificial vagina and electroejaculation in Brahman bulls.

    Science.gov (United States)

    Rego, J P A; Moura, A A; Nouwens, A S; McGowan, M R; Boe-Hansen, G B

    2015-09-01

    The present study was conducted to investigate if differences exist in the seminal plasma protein profile from mature Brahman bulls using two methods of semen collection: internal artificial vagina (IAV) and electroejaculation (EEJ). Semen was collected four times from three bulls on the same day and parameters were assessed immediately post-collection. Seminal plasma proteins were evaluated by 2-D fluorescence difference gel electrophoresis and identified by mass spectrometry. Semen volume was greater (P < 0.05) for EEJ (4.6 ± 0.35 mL) than for IAV (1.86 ± 0.24 mL) but sperm concentration was greater in IAV (1505 ± 189 × 10(6) sperm/mL) than in EEJ samples (344 ± 87 × 10(6) sperm/mL). Sperm motility and the percentage of normal sperm were not different between treatments. Total concentration of seminal plasma proteins was greater for samples collected by IAV as compared to EEJ (19.3 ± 0.9 compared with 13.0 ± 1.8 mg/mL, P < 0.05; respectively). Based on 2-D gels, 22 spots had a greater volume (P < 0.05) in gels derived from IAV samples, corresponding to 21 proteins identified as transferrin, albumin, epididymal secretory glutathione peroxidase, among others. Thirty-three spots, corresponding to 26 proteins, had a greater volume (P < 0.05) in gels derived from EEJ samples. These proteins were identified as spermadhesin-1, Bovine Sperm Protin 1, 3 and 5 isoforms, angiogenin-1, alpha-1B-glycoprotein, clusterin, nucleobindin-1, cathepsins, spermadhesin Z13, annexins, among others. Thus, proteins in greater amounts in samples obtained by IAV and EEJ were mainly of epididymal origin and accessory sex glands, respectively.

  3. Magnetic resonance imaging of primary lymphoma of the cervix

    Energy Technology Data Exchange (ETDEWEB)

    Marin, Carlos; Sanchez, Marisa; Ruiz, Yolanda; Garcia, Jose A. [Servicio de Radiodiagnostico, Hospital San Rafael, Madrid (Spain); Seoane, Jose M. [Servicio de Ginecologia, Seccion de Oncologia, Madrid (Spain)

    2002-06-01

    Although uterus and cervix infiltration is a frequent finding in the later stages of lymphomatous disease, primary lymphoma of the cervix is very uncommon; however, this entity can occasionally be distinguished from cervical carcinoma by means of MRI. This is an important fact as treatment and prognosis differ between these neoplasms. We present a case of primary cervical lymphoma studied with high-field (1.5 T) MRI and we make an extensive review of the literature. The cervical mass was found in a routine pelvic examination in a patient with no previous history of gynecologic disorders. This is an uncommon way of presentation of this disease. T2-weighted turbo spin-echo (TSE) sequences in the axial, sagittal, and coronal planes, and T1-weighted SE pre- and post-contrast images, were obtained. The lack of involvement of the mucosa, as well as sparing of cervical stroma and uterine junctional zone, are the most important findings to differentiate cervical lymphoma from carcinoma, and are best evaluated with T2 TSE sequences. Post-contrast images help to delineate the extent of the disease. (orig.)

  4. Unravelling the Bacterial Vaginosis-Associated Biofilm: A Multiplex Gardnerella vaginalis and Atopobium vaginae Fluorescence In Situ Hybridization Assay Using Peptide Nucleic Acid Probes.

    Science.gov (United States)

    Hardy, Liselotte; Jespers, Vicky; Dahchour, Nassira; Mwambarangwe, Lambert; Musengamana, Viateur; Vaneechoutte, Mario; Crucitti, Tania

    2015-01-01

    Bacterial vaginosis (BV), a condition defined by increased vaginal discharge without significant inflammation, is characterized by a change in the bacterial composition of the vagina. Lactobacillus spp., associated with a healthy vaginal microbiome, are outnumbered by BV-associated organisms. These bacteria could form a polymicrobial biofilm which allows them to persist in spite of antibiotic treatment. In this study, we examined the presence of Gardnerella vaginalis and Atopobium vaginae in vaginal biofilms using Peptide Nucleic Acid (PNA) probes targeting these bacteria. For this purpose, we developed three new PNA probes for A. vaginae. The most specific A. vaginae probe, AtoITM1, was selected and then used in an assay with two existing probes, Gard162 and BacUni-1, to evaluate multiplex FISH on clinical samples. Using quantitative polymerase chain reaction (qPCR) as the gold standard, we demonstrated a sensitivity of 66.7% (95% confidence interval: 54.5% - 77.1%) and a specificity of 89.4% (95% confidence interval: 76.1% - 96%) of the new AtoITM1 probe. FISH enabled us to show the presence of a polymicrobial biofilm in bacterial vaginosis, in which Atopobium vaginae is part of a Gardnerella vaginalis-dominated biofilm. We showed that the presence of this biofilm is associated with high bacterial loads of A. vaginae and G. vaginalis.

  5. ROLE OF PALLIATION IN STAGE IV CARCINOMA CERVIX

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    Smriti

    2013-03-01

    Full Text Available ABSTRACT: BACKGROUND: Palliation reduces the severity of disease symptoms, rather than reversing its progression or providing a cure. Metast atic cancer cervix (Ca Cx is incurable by surgery, radiation or chemotherapy, but these modali ties are useful for palliation. Globally about five to six lakh new cases of carcinoma cervi x are diagnosed every year. Of these, one lakh cases are diagnosed in India of which 25.0% are fro m West Bengal only. OBJECTIVES: Our objective was to study the role of palliation in Sta ge IV Carcinoma Cervix. SETTINGS AND DESIGN: During the study period of five years from January 2 007 to December 2011, consecutive seventy five new cases of stage IV carc inoma cervix diagnosed at Netaji Subhas Chandra Bose Cancer Research Institute, Kolkata, we re included in our study. MATERIALS AND METHODS: Clinical examination with relevant investigations l ike kidney function tests (KFT, biopsy, cystoscopy, CT scan etc were done for diagno sis & staging. Treatment was decided based on woman's age, general health and the locati on & type of the tumour. Treatment options were surgery, radiotherapy (RT, chemotherapy (CT an d simple palliation. In our study, combined CT+RT was done in 18.67% patients most of w ho presented with Stage IV disease. Radiation was given as brachytherapy following telet herapy. Chemotherapy was used as adjunct to RT or for palliation or as neo-adjuvant c hemotherapy (NACT, most commonly using paclitaxel (135mg/square metre, cisplatin (50mg/ squ are metre and 5- fluorouracil (600mg/ square metre. At times, chemotherapy could provide pa in relief only. Vault smear and metastatic workup was done during follow-up visits ev ery 8-12 weeks after treatment completion. RESULTS: Majority of patients belonged to the age group 42-69 years with a median age of 53 years. Bladder involvement was see n in 15(20.0% cases, bowel involvement in 14(19.0% and distant metastasis in 46(61.0% ca ses. Most cases were of Squamous

  6. A STUDY ON THE ROLE OF MICRO NUCLEI IN ASSESSING THE PROGRESSION OF PRECANCEROUS LESIONS OF CERVIX AND THE DIAGNOSIS OF CARCINOMA OF CERVIX

    Directory of Open Access Journals (Sweden)

    Anitha

    2015-06-01

    Full Text Available BACKGROUND: Invasive squamous cell carcinoma of cervix remains the most common malignant neoplasm of the female genital tract in many countries. The Papanicolaou stained cervical smear is an excellent and effective method in the diagnosis of invasive carcinoma and precancerous lesions of the cervix. This study was conducted to assess the value of Feulgen stained cervical smears in elucidating nuclear features help ful in the diagnosis of malignancy over conventional Pap stained smears and also to look for micronucleated cells in suspected cases of carcinoma of cervix. AIM: To analyse the distribution of cases of invasive squamous cell carcinoma and intraepithelial n eoplasia (squamous intraepithelial lesion of cervix over a period of 3 years, to elucidate additional nuclear features helpful in diagnosis of carcinoma using Feulgen stained cervical smears and to study the distribution of micronuclei in Feulgen stained smears from suspected cases of Carcinoma cervix. MATERIALS AND METHODS: A three year analysis of all cases of dysplasia and invasive carcinoma of cervix was done by reviewing Papanicolaou stained cervical smears from all the diagnosed cases of invasive car cinoma and precancerous lesions of the cervix. Cervical smears from sixty clinically suspected cases of carcinoma of cervix and smears from 10 normal women collected during a period of 12 months were studied in detail using Papanicolaou stained and Feulgen Stained Smears and micronuclei analysis (MN analysis was done. RESULTS : A total of 24343 cervical smears were screened during the 3 year period of study. Out of these 24343 cases there were 267(1.09% cases of cervical intraepithelial neoplasia and 144(0 .592% cases of invasive squamous cell carcinoma. Micronuclei analysis done using Feulgen Stained Smears demonstrated a consistent increase in micronucleated cells proportional to the increase in severity of th e lesion from normal to invasive carcinoma. CO NCLUSION : This study

  7. El simbolismo de la vagina terrestre: metaforas de la fertilidad, el nacimiento y la muerte en mesoamerica

    OpenAIRE

    Báez-Jorge, Félix

    2013-01-01

    Note portant sur l'auteur REFERENTES MÍTICOS Y CONTEXTOS SIMBÓLICOS En este ensayo realizo una nueva lectura de registros etnográficos, arqueológicos, epigráficos y documentales en torno al simbolismo de la vagina terrestre en el ámbito de la cosmovisión mesoamericana. Lo conceptualizo en tanto condensación dialéctica de diversos significados expresados en imágenes análogas. Incursiono en relatos míticos convergentes, puentes simbólicos entre la mujer y la tierra, pretendiendo trazar un arco ...

  8. Laparoscopy in the treatment of cancer of the corpus uteri in patients with metabolic syndrome. A case report

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    A. I. Berishvili

    2015-01-01

    Full Text Available Today, the common term metabolic syndrome encompasses visceral (abdominal obesity, glucose intolerance, type 2 diabetes mellitus (DM, hypertension, and dyslipidemia. In Europe, the rate of obesity mong the women ranges from 6 to 36 %. In the USA, 65 % of the adult population is overweight and 30 % is obese. High estrogen production in adipose tissue in patients with obesity has been established to increase the risk of cancer of the corpus uteri (CCU by 4 times as compared to that in normal weight female patients. Furthermore, obese patients are at increased risk for DM that is also a risk factor for CCU. A rise in the number of obese patients leads to the population redistribution of gynecological cancer. The increasing number of patients with gynecological cancer is overweight. This patient group has an increased risk from surgery and anesthesia, a higher incidence of postoperative complications, and delayed recovery. Laparotomy increases the duration of hospital stay and the rate of wound complications. The frequency of urologic injuries in obese patients varies between 2 and 4 %. In this patient category, the rate of damage to the large vessels or bowels has also statistically significant differences. The literature highlights a number of complications specific to patients with obesity: trocar damage to anterior abdominal wall vessels, particularly to the inferior epigastric artery, urinary bladder, as well as trocar site hernia. The authors describe their experience in treating CCU patients with metabolic syndrome at the Department of Surgery for Female Reproductive System Tumors, N. N. Blokhin Russian Cancer Research Center. They demonstrate that laparoscopic extirpation of the uterus and its appendages can be performed in patients with third-degree obesity (weighing 174 kg in Stage I CCU. It should be noted that this weight was previously regarded as an absolute contraindication to surgical treatment. Today, the active

  9. Melanosis of the uterine cervix: a case report and literature review

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    Daniel Chang

    2013-06-01

    Full Text Available Melanosis of the uterine cervix is an extremely rare melanocytic lesion and should be differentiated from melanoma. It is a melanocytic hyperpigmentation of basal layer cells from the squamous mucosa of the uterine cervix without an increase in the number of melanocytes. We present a typical case of this entity. Furthermore, we discuss the possible origins of melanocytes in this region and their association with Laugier-Hunziker syndrome and Carney complex.

  10. Collagen and glycosaminoglycan profiles in the canine cervix during different stages of the estrous cycle and in open- and closed-cervix pyometra.

    Science.gov (United States)

    Linharattanaruksa, Pichanun; Srisuwatanasagul, Sayamon; Ponglowhapan, Suppawiwat; Khalid, Muhammad; Chatdarong, Kaywalee

    2014-03-01

    The extracellular matrix of the cervix that comprises collagen, elastin, proteoglycans and glycosaminoglycans (GAGs) is thought to have an essential role in cervical relaxation. This study investigated the proportion of collagen and smooth muscle as well as the GAGs in cervices obtained from healthy bitches at different stages of the estrous cycle and bitches with open- and closed-cervix pyometra. Cervices were collected after ovariohysterectomy. The proportion of collagen to smooth muscle was determined using Masson's trichrome staining. Alcian blue staining was used to evaluate the relative distribution of cervical GAGs. The proportion of cervical collagen relative to smooth muscle was higher at estrus compared to anestrus (P≤0.05). It was also higher (P≤0.05) in bitches with open- compared to those with closed-cervix pyometra. Overall, hyaluronan (HA) was the predominant GAG in the canine cervix. In the luminal epithelium, the staining intensity for HA was stronger in estrus than in anestrus (P≤0.05), but not in diestrus (P>0.05). On the contrary, the intensity for the combined keratan sulfate (KS) and heparan sulfate (HS) was stronger in anestrus than in estrus and diestrus (P≤0.05). In bitches with pyometra, the staining intensity of the stroma for KS and HS was weaker in open- compared to closed-cervix pyometra (P≤0.05). Collectively, the different profiles of collagen and GAG suggest that the metabolism of both collagen and GAGs in the canine cervix is associated with hormonal statuses during the estrous cycle and cervical patency of bitches with pathological uterine conditions, such as pyometra.

  11. Sobrevida en pacientes con cáncer de cervix

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    Adolfo Ortiz-Barboza

    2005-10-01

    Full Text Available Objetivo: Estimar la probabilidad acumulada de sobrevida a cinco años, de una cohorte de pacientes diagnosticadas con cáncer de cervix durante 1999. Metodología: Para el análisis de la información se utilizaron distribuciones de frecuencia con valores absolutos y porcentuales, medidas de tendencia central y de dispersión. La relación entre variables cualitativas se efectuó mediante la prueba estadística de Chi cuadrado (X2. El nivel de significancia se fijó en p < 0.05. La probabilidad acumulada de sobrevida fue realizada mediante el método de Kaplan-Meier. La comparación entre los tiempos de sobrevida se realizó mediante el test de rangos logaritrnicoso El nivel de significancia fue fijado en p < 0,05. Resultados: Se analizaron 778 casos al final del periodo de estudio fallecieron 91 pacientes. La probabilidad acumulada de sobrevida a los cinco años para toda la población fue del 88,3%. Las pacientes a las cuales se les detectó carcinoma in situ al momento del diagnóstico, presentaron una probabilidad acumulada de sobrevida de 98,8%, mientras que a aquellas a quienes se les detectó un carcinoma invasor, fue del 68,3%. Esta diferencia fue estadísticamente significativa. La probabilidad acumulada de sobrevida para las pacientes diagnosticadas con carcinoma de células escamosas fue de un 89%, mientras que para aquellas diagnosticadas con adenocarcinoma fue de un 80%. Esta diferencia no fue estadísticamente significativa. Conclusiones: Los resultados obtenidos muestran valores superiores a los determinados en otros países con un nivel de desarrollo inferior al nuestro, y similares a los encontrados en naciones desarrolladas. Con los resultados obtenidos, se podría suponer que las probabilidades acumuladas de sobrevida son mayores en aquellas pacientes a quienes se les realiza el diagnóstico de cáncer de cervix en edades tempranas, y que esto puede relacionarse con la alta proporción de carcinomas in situ diagnosticados en

  12. Optimization of combined radiation therapy of the cervix cancer

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    Vladimir Philippenko

    2010-04-01

    Full Text Available Use of a new combination of known medical products - inhibitorsenzyme of cyclooxigenase-2 (diclofenac, ketoprofen with smalldoses cytostatics (methotrexate, 5-fluorouracil as“nonconventional” radiosensibilizators for optimization of combinedradial treatment of cervical cancer is offered. One hundred andtwenty patients with cervix cancer were involved into research(average age - 52.5±3.3, mainly II stage of process (50.8±4.6%,morphologically - nonkeratinizing squamous cell carcinoma(65.0±4.4%. Frequency of full regress of a tumor in the basicgroups has reached in 77.5±6.6% (1-basic group and 82.5±6.0% (2-basic group in comparison with a control group 70.0±7.2%(р<0.05. By results of the cytological research in cells thepathomorphosis of IV degree was recorded in 1-basic group - 60.0%(superficial smears and 57.5% (a puncture biopsy, in 2-basic group- 85.0% (superficial smears and 82.5% (a puncture biopsy incomparison with the control - 55.0% (superficial smears and apuncture biopsy, р<0.05.

  13. Age as a prognostic factor in carcinoma of the cervix.

    Science.gov (United States)

    Lybeert, M L; Meerwaldt, J H; van Putten, W L

    1987-06-01

    To investigate whether age is a prognostic factor in patients with carcinoma of the cervix, a retrospective study was undertaken of 261 patients, aged 45 years or less, who were referred to the Rotterdamsch Radio-Therapeutisch Instituut (RRTI) between 1973 and 1982. Patients were referred for either primary treatment--surgery or radiotherapy--or for adjuvant radiotherapy. Overall 5-year survival figures were rather low, which may be explained by negative patient selection as the RRTI is a referral hospital: stage IB, 72%; stage IIA; 61%; stage IIB; 52%; stage III; 29%. A particular poor survival was noted for patients (n = 22) aged 28 or less. Overall 5-year survival of these patients was only 39% in contrast to 67% 5-year survival of older patients. This difference was highly significant (p less than 0.002). Even if corrected for stage, very young patients had a poorer prognosis (stage IB: 45% versus 75% 5-year survival of older patients). Within the older age group, no trend towards a better prognosis with increasing age could be identified. As a treatment was similar for all patients, no explanation is available for this observation.

  14. [Morphologic and biochemical aspects of prostaglandin-induced cervix ripening].

    Science.gov (United States)

    Rath, W; Adelmann-Grill, B C; Schauer, A; Kuhn, W

    1987-01-01

    For examination by electron microscopy tissue samples were taken from the posterior lip of the cervix of 8 patients having a termination of pregnancy at 9-12th week gestation and 16 patients of comparable gestational age who had an intracervical application of either 2 ml 5% tylose or 50 micrograms sulprostone-tylose gel 8 hours before biopsy. The efficacy of cervical priming was demonstrated objectively by tonometric studies. In addition, collagenase and protease activities were determined in the cervical tissue extracts of the different treatment groups. For identification of typical collagen fragments SDS-polyacrylamide-gel-electrophoresis were carried out on the acetic acid soluble extracts. The local application of sulprostone gel induced a marked multifocal loosening of the collagenous framework; there was no evidence for leucocyte infiltration or necrosis caused by the prostaglandin (PG) pretreatment. In the areas of disorganized collagen fibres cervical fibroblasts seemed to be activated characterized by fine granular loosening of the cytoplasma, dilated cisternae of rough endoplasmatic reticulum, vacuolized enlarged mitochondria and an increased number of cytoplasmatic vesicles close to the cell surface. Collagenase and protease activities were found in all extracts of the different treatment groups, however, PG-application led to no significant increase in enzymatic activities. There was no evidence for the presence of typical collagen cleavage products in the SDS-electrophoresis. Contradictory to the hitherto published literature enzymatic collagen degradation does not play an essential role in PG-induced cervical ripening.

  15. Perbedaan Ekspresi P16INK4a dan HPVL1 pada Cervical Intraepithelial Neoplasia 1, Cervical Intraepithelial Neoplasia 2, Cervical Intraepithelial Neoplasia 3 dan Squamous Cell Carcinoma Serviks Uteri

    Directory of Open Access Journals (Sweden)

    Arlene Elizabeth Padang

    2014-09-01

    Full Text Available Human papillomavirus (HPV memegang peranan penting dalam proses karsinogenesis kanker serviksuteri; namun hanya sebagian kecil wanita yang terinfeksi tersebut akan berkembang menjadi kankerserviks yang invasif. Cervical intraepithelial neoplasia (CIN merupakan spektrum dari lesi servikalyang mewakili lesi prekursor dari squamous cell carcinoma (SCC serviks uteri yang dikategorikanmenjadi CIN1, CIN2, CIN3. Interaksi protein HPV (E6 dan E7 dengan protein pengatur selular (pRbdan p53 akan menyebabkan up regulation protein P16INK4a. P16INK4a merupakan tumor supresorprotein cyclin dependen kinase inhibitor yang menghambat cyclin dependent kinase 4 dan 6 yangmerupakan produk dari gen INK4a yang terlibat dalam fosforilasi protein retinoblastoma (pRb.Human papillomavirus-late 1 (HPVL1 merupakan protein kapsid yang terekspresi pada saat awalfase produktif karsinogenesis serviks uteri. Tujuan dari penelitian ini adalah untuk mengetahuiperbedaan ekspresi protein P16INK4a dan HPVL1 pada CIN1, CIN2, CIN3, dan SCC serviks uteri,dimana ekspresi P16INK4a dapat membantu untuk membedakan berbagai derajat displasia serviksuteri dan ekspresi HPVL1 dapat membantu untuk memprediksi progresivitas dari berbagai derajatdisplasia serviks uteri, sehingga penanganan pasien menjadi lebih tepat. [MEDICINA 2013;44:77-81].

  16. The possible role of female sex hormones in milk from pregnant cows in the development of breast, ovarian and corpus uteri cancers.

    Science.gov (United States)

    Ganmaa, Davaasambuu; Sato, Akio

    2005-01-01

    The continued increase in incidence of some hormone-related cancers worldwide is of great concern. Although estrogen-like substances in the environment were blamed for this increase, the possible role of endogenous estrogens from food has not been widely discussed. We are particularly concerned about cows' milk, which contains a considerable quantity of estrogens. When we name cows' milk as one of the important routes of human exposure to estrogens, the general response of Western people is that "man has been drinking cows' milk for around 2000 years without apparent harm." However, the milk that we are now consuming is quite different from that consumed 100 years ago. Unlike their pasture-fed counterparts of 100 years ago, modern dairy cows are usually pregnant and continue to lactate during the latter half of pregnancy, when the concentration of estrogens in blood, and hence in milk, increases. The correlation of incidence and mortality rates with environmental variables in worldwide countries provides useful clues to the etiology of cancer. In this study, we correlated incidence rates for breast, ovarian, and corpus uteri cancers (1993-97 from Cancer Incidence in Five Continents) with food intake (1961-97 from FAOSTAT) in 40 countries. Meat was most closely correlated with the breast cancer incidence (r=0.827), followed by milk (0.817) and cheese (0.751). Stepwise multiple-regression analysis (SMRA) identified meat as the factor contributing most greatly to the incidence of breast cancer ([R]=0.862). Milk was most closely correlated with the incidence of ovarian cancer (r=0.779), followed by animal fats (0.717) and cheese (0.697). SMRA revealed that milk plus cheese make the greatest contribution to the incidence of ovarian cancer ([R]=0.767). Milk was most closely correlated with corpus uteri cancer (r=0.814), followed by cheese (0.787). SMRA revealed that milk plus cheese make the most significant contribution to the incidence of corpus uteri cancer ([R]=0

  17. Rescue and nursing of vaginal acute massive hemorrhage of uterine cervix cancer patients%宫颈癌阴道急性大出血患者的抢救及护理

    Institute of Scientific and Technical Information of China (English)

    陈伟月; 梁平; 余勇妙; 梁志群

    2009-01-01

    Objective To explore the rescue and nursing of vaginal acute massive hemorrhage of uterine cervix cancer patients. Methods The clinical data of 12 uterine cervix cancer patients with vaginal acute massive hemorrhage were analyzed retrospectively. The measures were included: ①Rescne shock: establishing two intravenoun pathways quickly, checking blood type, crees matching and rapid expanding the blood volume; ②Hemostasia as possible: rapid hemostasia by vagina packing with sterile or iodoform gauze, reducing the psychentonia of patients and receiving antibiotic treatment; ③Oxygen inhalation; ④Cloee observation: observing the change of vaginal hemorrhage and vital signs closely; ⑤ Strengthening the basic nursing and preventing the complication. Results All patients were cured in time. After anti - inflammation, hemostasia, operation and radiotherapy, all patients were discharged from hospital or received radiotherapy in ontology department. Conclusions The right nursing measures and hemostasia as possible are the key to rescuer the uterine cervix cancer patients with vaginal acute massive hemorrhage.%目的 探讨宫颈癌阴道急性大出血患者的抢救及护理有关问题.方法 回顾分析12例宫颈癌阴道急性大出血患者的临床资料、抢救及护理过程,措施包括:①抢救休克:快速建立两条静脉通道、查血型及交叉配血,快速扩充血容量;②迅速止血:迅速阴道内填塞无菌纱布或碘仿纱条压迫止血,减少精神紧张等因素,给予抗生素;③氧气吸入;④密切观察:密切观察阴道流血及生命体征变化,对症施护;⑤加强基础护理、预防并发症.结果患者得到及时的救治,经抗炎、止血、手术、放射治疗,全部患者康复出院或转肿瘤科继续放疗.结论 宫颈癌引起阴道急性大流血,争取时机尽快止血、护理措施正确及时是抢救成功的关键.

  18. Ultrastructural study of the neovagina following the utilization of human amniotic membrane for treatment of congenital absence of the vagina

    Directory of Open Access Journals (Sweden)

    L.F. Bleggi-Torres

    1997-07-01

    Full Text Available We present an ultrastructural study of the utilization of human amniotic membrane in the treatment of congenital absence of the vagina in 10 patients. All patients were surgically treated with application of an amniotic membrane graft using the modified McIndoe and Bannister technique. Sixty days after surgery, samples of the vaginal neo-epithelium were collected for transmission electron microscopy analysis. The ultrastructural findings consisted of a lining of mature squamous epithelium indicating the occurrence of metaplasia of the amniotic epithelium into the vaginal epithelium. The cells were arranged in layers as in the normal vaginal epithelium, i.e., superficial, intermediate and deep layers. There were desmosomes and cytoplasmic intermediate cytokeratin filaments, as well as some remnant features of the previous amniotic epithelium. These findings suggest that human amniotic membrane is able to complete metaplasia into squamous cells but the mechanism of this cellular transformation is unknown

  19. Analysis of study rezults of biopsies of the vagina mucous membrane in women with recurrent genital prolapse

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    Banakhevich R.M.

    2014-06-01

    Full Text Available The study involved 53 women with recurrent genital prolapse. The average age of the women studied was 62,8±9,8 years, age of women of the control group was 57,2±6,3 years (p>0,05. Features of expression of estrogen and progesterone receptors levels, markers of cell proliferation and collagen in the biopsy of the vagina mucous membrane were estimated. In the vaginal mucosa of patients with recurrent prolapse expression of Ki-67 markers of proliferative activity was not established. According to the study general lowering of estrogen receptors by 76,2% in patients with recurrent genital prolapse was established, whereas in patients of the control group level of estrogen receptors in the unchanged epithelium and stroma was positive in 100% of cases. In patients with recurrent genital prolapse the decrease of expression of progesterone receptors by 83,4% was identified.

  20. Genital panics: constructing the vagina in women's qualitative narratives about pubic hair, menstrual sex, and vaginal self-image.

    Science.gov (United States)

    Fahs, Breanne

    2014-06-01

    An emerging body of research targets women's relationship to their genitals, particularly as pubic hair removal and the promotion of female genital surgeries increase in popularity and visibility. This study asked women to discuss their subjective feelings about three related but distinct genital attitudes: pubic hair grooming, sex during menstruation, and genital/vaginal self-image. Specifically, this study applied thematic analysis to qualitative interviews with a community sample of 20 women (mean age=34, SD=13.35) from diverse ages, races, and sexual identity backgrounds to illuminate seven themes in women's narratives about their vaginas: (1) "dirty" or "gross"; (2) needing maintenance; (3) unknown or frustrating; (4) unnatural; (5) comparative; (6) ambivalent; (7) affirmative. Overwhelmingly, women used strong emotional language when discussing their genitals, often evoking descriptions of anxiety, excess, and need for control. Fusions between sexuality and body image, and connections between "genital panics" and internalized racism, sexism, and homophobia, also appeared.

  1. Two-dimensional Electrophoresis Analysis of Differential Protein Expression in Squamous Carcinoma of the Cervix

    Institute of Scientific and Technical Information of China (English)

    ZHU Xue-qiong; WU Jie-li; YU Li-rong; LIN Yi; L(U) Jie-qiang; ZOU Shuang-wei; HU Yue

    2008-01-01

    Objective:To establish and optimize the two-dimensional gel electrophoresis(2-DE)maps of squamous carcinoma of the cervix and to study the protein difference between squamous carcinoma of the cervix(SCC)and normal cervical tissue.Methods:Using Two-dimensional gel electrophoresis followed by computer-assisted image analysis,the differential proteins between squamous carcinoma of the cervical tissue and normal cervical tissue were compared.Then using matrix-assisted laser desorption/ionization-time of flight mass spectrometry,the differential proteins were identified.Results:The well-resolved and reproducible two-dimensional gel electrophoresis patterns of squamous carcinoma of the cervix tissue and normal cervical tissue were obtained.After silver staining.the average matching ratio of squamous carcinoma of the cervix was 86.1%.There was a good reproducibility of spot position in 2-DE map,with average deviation in IEF direction of 0.95±0.13 mm,while in SDS-PAGE direction it was 1.20±0.18 mm.Ten protein spots were identified by mass spectrometry,some of which were involved in cell proliferation,cell apoptosis,intracellular enzymes,structural proteins,cycle regulation,and tumor occurrence.Conclusion:The differentially expressed proteins provide a fundamental basis for further study of human squamous carcinoma of the cervix and screening of its specific markers.

  2. MR imaging of the uterus and cervix in healthy women: Determination of normal values

    Energy Technology Data Exchange (ETDEWEB)

    Hauth, Elke A.M.; Libera, Hanna; Lange, Silke; Forsting, Michael [University Hospital Essen, Department of Diagnostic and Interventional Radiology and Neuroradiology, Essen (Germany); Jaeger, Horst J. [Neuroradiology and Nuclear Medicine, Marien-Hospital Wesel, Department of Radiology, Wesel (Germany)

    2007-03-15

    The purpose of this study was to establish normal values for the volume of the uterus and cervix in MRI based on age and the menstrual cycle phase. We performed MRI of the pelvis in 100 healthy women. For the uterus, they were further divided into two groups: one with myomas and/or adenomyosis and one without either. The volume of the uterus and cervix and thickness of the uterine wall layers were analysed by age and the menstrual cycle phase. The mean volume of the uterus in both groups and the cervix significantly increased with age to reach its peak at 41-50 years, and then dropped. Likewise, the thickness of the endometrium and the junctional zone, but not the myometrium, significantly increased until 41-50 years, and then decreased. When we compared the volume of the uterus and cervix and the thickness of the uterine wall layers between the two phases of the menstrual cycle, we found no significant differences. The volume of the uterus and cervix and the thickness of the endometrium and junctional zone differ significantly with age, but not between the two phases of the menstrual cycle. Knowledge of MRI-related normal values can be expected to aid the early identification of uterine pathologies. (orig.)

  3. A COMPARATIVE STUDY OF CAREGIVER BURDEN IN CANCER CERVIX AND CANCER BREAST ILLNESSES

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    Srinivasagopalan, Nappinnai, Solayappan

    2015-07-01

    Full Text Available Background: Caregivers of individuals suffering from cancer illnesses are at risk of having subjected to mental health consequences. There is a paucity of data comparing the caregiver burden of cancer breast and cancer cervix patients. Aim: The aim of the present study is to compare the caregiver burden of cancer breast and cancer cervix patients. To study the association of caregiver burden with demographic factors like age, gender, duration of caregiving etc. Materials & Methods: This Cross sectional study is performed on the key relatives of patients of 31 cancer cervix and 31 cancer breast patients. Burden assessment schedule was used. Results: Our findings suggest burden is more in male caregivers of breast cancer patients. It is not so in caregivers of cancer cervix patients. Whenever the caregiver is closely related to the patients the burden is high in both groups. Whenever the burden scores were high the depression scores were also high. Treatment modalities as a whole correlates with burden scores in caregivers of breast cancer patients but not in cancer cervix patients. Conclusion: Caregivers with breast and cervical cancer patients are vulnerable if the caregiver is male, from low socioeconomical background, more closely related and when the patients received poor treatment modalities.

  4. Correlation of human papilloma virus presence with precancerous and cancerous lesions of uterine cervix by immunohistochemistry

    Directory of Open Access Journals (Sweden)

    Meenu Pujani

    2012-01-01

    Full Text Available Background : Cancer of cervix is the most common form of cancer in females of developing countries. Cervical cancer is the best example of common human malignancy with a proven infectious etiology. The data linking human papilloma virus (HPV infection with the epidemiology and pathogenesis of cervical neoplasia is convincing. There are various methods for detection of HPV like immunohistochemistry, polymerase chain reaction, liquid phase hybridization (hybrid capture test, in situ hybridization etc. Materials and Methods: We studied the profile of precancerous and cancerous lesions of uterine cervix and correlated human papilloma virus (HPV presence with precancerous and cancerous lesions of uterine cervix by immunohistochemistry. Total 50 cases were first studied, analyzed, and classified histologically and then immunohistochemistry was done. Results : Majority of the cases 36/50 (72% under study are squamous cell carcinomas, which is the most common carcinoma found in the uterine cervix. HPV positivity for all cases of cervical carcinoma was found to be 34.7% (16/46 cases, for carcinoma in situ, it was 50% (2/4 cases. It can be thus hypothesized that with decreasing differentiation, there is loss of expression of HPV in the cervical epithelial cells. Conclusion: To conclude, though this study confirms the correlation of HPV presence with precancerous and cancerous lesions of uterine cervix, it also suggests that there must be other co-factors involved in cervical carcinogenesis as well.

  5. Characterization of microbial communities found in the human vagina by analysis of terminal restriction fragment length polymorphisms of 16S rRNA genes

    NARCIS (Netherlands)

    Coolen, MJL; Post, E; Davis, CC; Forney, LJ

    2005-01-01

    To define and monitor the structure of microbial communities found in the human vagina, a cultivation-independent approach based on analyses of terminal restriction fragment length polymorphisms (T-RFLP) of 16S rRNA genes was developed and validated. Sixteen bacterial strains commonly found in the h

  6. Diversity of the Lactobacillus group in breast milk and vagina of healthy women and potential role in the colonization of the infant gut

    NARCIS (Netherlands)

    Martin, R.; Heilig, G.H.J.; Zoetendal, E.G.; Smidt, H.; Rodriguez, J.M.

    2007-01-01

    Aims: To evaluate the diversity of the Lactobacillus group in breast milk and the vagina of healthy women and understand their potential role in the infant gut colonization using the 16S rRNA gene approaches. Methods and Results: Samples of breast milk, vaginal swabs and infant faeces were aseptical

  7. Vibrational spectroscopy studies of formalin-fixed cervix tissues.

    Science.gov (United States)

    Krishna, C M; Sockalingum, G D; Vadhiraja, B M; Maheedhar, K; Rao, A C K; Rao, L; Venteo, L; Pluot, M; Fernandes, D J; Vidyasagar, M S; Kartha, V B; Manfait, M

    2007-02-15

    Optical histopathology is fast emerging as a potential tool in cancer diagnosis. Fresh tissues in saline are ideal samples for optical histopathology. However, evaluation of suitability of ex vivo handled tissues is necessitated because of severe constraints in sample procurement, handling, and other associated problems with fresh tissues. Among these methods, formalin-fixed samples are shown to be suitable for optical histopathology. However, it is necessary to further evaluate this method from the point of view discriminating tissues with minute biochemical variations. A pilot Raman and Fourier transform infrared (FTIR) microspectroscopic studies of formalin-fixed tissues normal, malignant, and after-2-fractions of radiotherapy from the same malignant cervix subjects were carried out, with an aim to explore the feasibility of discriminating these tissues, especially the tissues after-2-fractions of radiotherapy from other two groups. Raman and FTIR spectra exhibit large differences for normal and malignant tissues and subtle differences are seen between malignant and after-2-fractions of radiotherapy tissues. Spectral data were analyzed by principal component analysis (PCA) and it provided good discrimination of normal and malignant tissues. PCA of data of three tissues, normal, malignant, and 2-fractions after radiotherapy, gave two clusters corresponding to normal and malignant + after-2-fractions of radiotherapy tissues. A second step of PCA was required to achieve discrimination between malignant and after-2-fractions of radiotherapy tissues. Hence, this study not only further supports the use of formalin-fixed tissues in optical histopathology, especially from Raman spectroscopy point of view, it also indicates feasibility of discriminating tissues with minute biochemical differences such as malignant and after-2-fractions of radiotherapy.

  8. Pseudoneoplastic glandular lesions of the uterine cervix: a selective review.

    Science.gov (United States)

    Nucci, Marisa R

    2014-07-01

    Pseudoneoplastic glandular lesions of the cervix continue to be diagnostically challenging for the surgical pathologist. This review covers a select number of these lesions that may be misinterpreted as premalignant or malignant, with an emphasis on those about which Dr Scully has advanced our knowledge. The topics covered include microglandular hyperplasia, mesonephric hyperplasia, diffuse laminar endocervical glandular hyperplasia, lobular endocervical glandular hyperplasia, and endocervical adenomyoma. The first listed entity has a greater diversity of morphology than the name might imply including, but not limited to solid growth and prominent hyaline stroma. The second entity may be remarkably diffuse within the cervical wall and reasonably result in consideration of diagnoses such as minimal deviation adenocarcinoma (adenoma malignum), but has nonmucinous epithelium and bland cytology. The third entity, one of the least common of those considered, represents a peculiar form of reactive hyperplasia of the endocervical epithelium. The fourth entity is the one about which knowledge is still fast advancing. In pure form with no atypia it is almost certainly a clinically benign process, but a subset of cases show cytologic atypia and an occasional association with adenocarcinoma is seen. Finally, adenomyomas of the uterus in general have received much attention in recent years, mostly in the corpus, but the less common endocervical variant may be particularly problematic because mucinous epithelium in abundant myogenic stroma may be potentially confused with an infiltrating differentiated mucinous adenocarcinoma. Although immunohistochemistry may play a role on occasion in evaluating benign endocervical glandular proliferations the mainstay of their interpretation remains conventional morphologic analysis of routinely stained slides.

  9. Coexisting Malignant Melanoma and Blue Nevus of the Uterine Cervix: An Unusual Combination

    Directory of Open Access Journals (Sweden)

    David Parada

    2012-01-01

    Full Text Available Malignant melanoma (MM and blue nevi of the uterine cervix are an extremely rare neoplasm, probably derived from embryologic migration of melanocytes from the neural crest. MM displays aggressive behavior with a poor prognosis. We report the case of a 76-year-old postmenopausal woman abnormal vaginal bleeding. She underwent a hysterectomy and bilateral salpingo-oophorectomy with paraaortic-iliac lymphadenectomy. Histopathological and immunohistochemical studies were consistent with the diagnosis of MM and blue nevi in the uterine cervix. Although it is extremely rare, this case suggests that MM of the uterine cervix should be considered in the differential diagnosis of undifferentiated neoplasm. Early diagnosis is essential in order to warrant a better prognosis, although there are no cases of cure described.

  10. Primary plasmacytoma of the cervix in a 21-year-old female patient.

    Science.gov (United States)

    Schor, Ana Paula T; Moraes, Marcelo P T; Bisson, Frank W; Bisson, Marcelo A M; Luiz, Orlando M R; Bacchi, Carlos E

    2010-05-01

    Extramedullary (extraosseous) plasmacytomas are localized, plasma cell neoplasms that arise in tissues other than bone and bone marrow, and constitute about 4% of all plasma cell neoplasms. Extramedullary (extraosseous) plasmacytomas rarely affects the female lower genital tract; only 6 cases of primary cervix plasmacytomas have been reported to date. Here we describe the case of an otherwise healthy 21-year-old woman who presented for a routine examination with no symptoms. A Pap smear showed an intense inflammatory process with some atypical cells. This was confirmed by microscopic examination of a biopsy, which revealed a metaplastic process of the cervix with a massive infiltration of plasma cells with mild atypia. The atypical plasma cells showed cytoplasmic lambda immunoglobulin light chain restriction with the absence of kappa light chains, indicative of monoclonality. The patient was extensively screened for systemic disease, including bone marrow biopsy, but the disease was restricted to the cervix.

  11. Intraarterial Scintigraphy in recurrent Cervix Cancer - The Evaluation of Radionuclide therapeutic Trials -

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Eun Young; Suh, Jin Suck; Park, Chang Yun; Lee, Jong Tae; Yoo, Hyung Sik [Yonsei University College of Medicine, Seoul (Korea, Republic of)

    1990-07-15

    We performed 17 intraarterial scintigraphies in six patients with recurrent cervix cancer. With Seldinger method, the agent (four different radiopharmaceuticals) was perfused at the same speed of infusion of anticancer drugs (25 cc/hour) through internal iliac artery. There were four different radiopharmaceuticals; {sup 131}I-Lipiodol, {sup 99m}Tc(Technetium)-HSA (Human Serum Albumin), {sup 99m}Tc-Sucralfate and {sup 99m}Tc-MAA (Macroaggregated Albumin). We evaluate the distribution pattern of radioactivity by the use of ratio of Tumor/Extratumor uptake (T/ET ratio). Our results reveals that {sup 99m}Tc-MAA scan showed the highest T/ET ratio and the other were not ideal agents for intraarterial therapy of recurrent cervix cancer. In conclusion, an ideal radioisotope and tracer which can block capillary, for example MAA, should be re-evaluated or produced in order to treat the patient with recurrent cervix cancer.

  12. Bony Calvarium as the Sole Site ofMetastases in Squamous Cell Carcinomaof the Uterine Cervix

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    Mohammad Mohammadianpanah

    2010-10-01

    Full Text Available Isolated skeletal metastasis to the bony calvarium is extremely rare in patients with squamous cell carcinoma of the uterine cervix. We describe the clinical and imaging findings in a case of squamous cell carcinoma of the uterine cervix with metastases tothe bony calvarium as the sole site of metastasis. The patient was a 65-year-old woman with squamous cell carcinoma of the uterine cervix, FIGO stage IIIb, whose initial treatement was chemoradiation therapy. After 22 sessions of external-beam radiation,she developed headaches. On physical examination she had skull bone tenderness. On plain skull X-ray, there were osteolytic bony lesions. Brain MRI showed multiple enhancing skull bone metatstses. Eventually, a whole body bone scintigraphy revealed isolated diffuse increased activity in the bony calvarium. In the literature review, wefound only three similar cases of cervical cancer with scalp metastases and involvement of the bony calvarium.

  13. A SONOGRAPHIC SHORT CERVIX AS THE ONLY CLINICAL MANIFESTATION OF INTRA-AMNIOTIC INFECTION

    Science.gov (United States)

    HASSAN, SONIA; ROMERO, ROBERTO; HENDLER, ISRAEL; GOMEZ, RICARDO; KHALEK, NAHLA; ESPINOZA, JIMMY; NIEN, JYH KAE; BERRY, STANLEY M.; BUJOLD, EMMANUEL; CAMACHO, NATALIA; SOROKIN, YORAM

    2006-01-01

    OBJECTIVE A sonographically short cervix is a powerful predictor of spontaneous preterm delivery. However, the etiology and optimal management of a patient with a short cervix in the mid-trimester of pregnancy remain uncertain. Microbial invasion of the amniotic cavity (MIAC) and intra-amniotic inflammation are frequently present in patients with spontaneous preterm labor or acute cervical insufficiency. This study was conducted to determine the rate of MIAC and intra-amniotic inflammation in patients with a cervical length <25 mm in the mid-trimester. STUDY DESIGN A retrospective cohort study was conducted of patients referred to our high risk clinic because of a sonographic short cervix or a history of a previous preterm birth. Amniocenteses were performed for the evaluation of MIAC and for karyotype analysis in patients with a short cervix. Fluid was cultured for aerobic and anaerobic bacteria, as well as genital mycoplasmas. Patients with MIAC were treated with antibiotics selected by their physician. RESULTS Of 152 patients with a short cervix at 14–24 weeks, 57 had amniotic fluid analysis. The prevalence of MIAC was 9% (5/57). Among these patients, the rate of preterm delivery (<32 weeks) was 40% (2/5). Microorganisms isolated from amniotic fluid included Ureaplasma urealyticum (n=4) and Fusobacterium nucleatum (n=1). Patients with a positive culture for Ureaplasma urealyticum received intravenous Azithromycin. Three patients with Ureaplasma urealyticum had a sterile amniotic fluid culture after treatment, and subsequently delivered at term. The patient with Fusobacterium nucleatum developed clinical chorioamnionitis and was induced. CONCLUSION 1) Sub-clinical MIAC was detected in 9% of patients with a sonographically short cervix (<25 mm); and 2) maternal parenteral treatment with antibiotics can eradicate MIAC caused by Ureaplasma urealyticum. This was associated with delivery at term in the three patients whose successful treatment was documented by

  14. Collagen and matrix metalloproteinase-2 and -9 in the ewe cervix during the estrous cycle.

    Science.gov (United States)

    Rodríguez-Piñón, M; Tasende, C; Casuriaga, D; Bielli, A; Genovese, P; Garófalo, E G

    2015-09-15

    The cervical collagen remodeling during the estrous cycle of the ewe was examined. The collagen concentration determined by a hydroxyproline assay and the area occupied by collagen fibers (%C), determined by van Gieson staining, were assessed in the cranial and caudal cervix of Corriedale ewes on Days 1 (n = 6), 6 (n = 5), or 13 (n = 6) after estrous detection (defined as Day 0). In addition, the gelatinase activity by in situ and SDS-PAGE gelatin zymographies and matrix metalloproteinase-2 and -9 (MMP-2 and MMP-9, respectively) expression by immunohistochemistry were determined. The collagen concentration and %C were lowest on Day 1 of the estrous cycle (P MMP-2 activity was highest (P MMP-2 trend to be highest (P = 0.0819). The MMP-2 activity was detected in 73% of the homogenized cervical samples, and its expression was mainly detected in active fibroblasts. By contrast, the MMP-9 activity was detected in 9% of the samples, and its scarce expression was associated with plasmocytes, macrophages, and lymphocytes. Matrix metalloproteinase-2 expression was maximal on Day 1 in the cranial cervix and on Day 13 in the caudal cervix and was lower in the cranial than in the caudal cervix (P MMP-2 expression that differed between the cranial and caudal cervix may reflect their different physiological roles. The decrease in the collagen content and increase in fibroblast MMP-2 activity in sheep cervix on Day 1 of the estrous cycle suggests that cervical dilation at estrus is due to the occurrence of collagen fiber degradation modulated by changes in periovulatory hormone levels.

  15. High signals in the uterine cervix on T2-weighted MRI sequences

    Energy Technology Data Exchange (ETDEWEB)

    Graef, De M.; Karam, R.; Daclin, P.Y.; Rouanet, J.P. [Department of Radiology, C.M.C. Beausoleil, 119 avenue de Lodeve, 34000 Montpellier (France); Juhan, V. [Department of Radiology, C.H.U. Timone, 13000 Marseille (France); Maubon, A.J. [Department of Radiology, C.H.U. Dupuytren, 87000 Limoges (France)

    2003-01-01

    The aim of this pictorial review was to illustrate the normal cervix appearance on T2-weighted images, and give a review of common or less common disorders of the uterine cervix that appear as high signal intensity lesions on T2-weighted sequences. Numerous aetiologies dominated by cervical cancer are reviewed and discussed. This gamut is obviously incomplete; however, radiologists who perform MR women's imaging should perform T2-weighted sequences in the sagittal plane regardless of the indication for pelvic MR. Those sequences will diagnose some previously unknown cervical cancers as well as many other unknown cervical or uterine lesions. (orig.)

  16. Immunolocalization of Prostaglandin E2 Receptor Subtype 4 (EP4 in the Cervix of Cyclic Bitches and Those with Pyometra

    Directory of Open Access Journals (Sweden)

    P Linharattanaruksa1, K Chatdarong1, S Ponglowhapan1, M Khalid3 and S Srisuwatanasagul2*

    2013-07-01

    Full Text Available Cervix is an important part of the reproductive tract; in non-pregnant animals it remains closed during anestrus and diestrus and is open only during estrus. In pathological conditions like pyometra, the cervix may be open or closed but the control mechanism is not clearly known. Prostaglandin E2 (PGE2 is considered to be involved in changes of extracellular matrix via coupling to prostaglandin E receptor subtype 4 (EP4. This study investigated the expression of EP4 in the cervices of bitches during different stages of estrous cycle and those with pyometra. After ovariohysterectomy, cervices were collected from anestrus (n=6, estrus (n=12 and diestrus (n=6, open- (n=10 and closed-cervix pyometra (n=10 bitches. Cervical EP4 expression was observed at all the layers and the stages but the differences in EP4 expression either among bitches in different stages of the estrous cycle and between open- and closed-cervix pyometra were limited to only surface epithelium (SE. In cyclic bitches during estrus and in open-cervix pyometra bitches, significantly higher (P<0.05 EP4 expression was found in SE of uterine part than vaginal part. In SE of the uterine part, the expression was higher in the bitches during estrus than in anestrus and diestrus, and in the bitches affected by open-cervix than those with closed-cervix pyometra. The results suggest that regulation of cervical dilation appeared in the uterine part of the cervix. Moreover, EP4 may be involved in stimulating dilation of the cervix in both estrus and open-cervix pyometra bitches.

  17. Abnormalities of uterine cervix in women with inflammatory bowel disease

    Institute of Scientific and Technical Information of China (English)

    Jyoti Bhatia; Panayota Kotsali; Oana Vele; Jason Bratcher; Burton Korelitz; Katherine Vakher; Shlomo Mannor; Maria Shevchuk; Gworgia Panagopoulos; Adam Ofer; Ecaterina Tamas

    2006-01-01

    AIM: To evaluate the prevalence of abnormalities of the uterine cervix in women with inflammatory bowel disease (IBD) when compared to healthy controls.METHODS: One hundred and sixteen patients with IBD [64 with Crohn's disease (CD) and 52 with ulcerative colitis (UC)] were matched to 116 healthy controls by age (+/- 2 years) at the time of most recent papanicolaou (Pap) smear. Data collected consisted of age, race, marital status, number of pregnancies,abortions/miscarriages, duration and severity of IBD,Pap smear results within five years of enrollment, and treatment with immunosuppressive drugs. Pap smear results were categorized as normal or abnormal including atypical squamous cells of undetermined significance (ASCUS), low-grade squamous intraepithelial lesion (LGSIL), and high-grade squamous intraepithelial lesion (HGSIL). RESULTS: The median age at the time of Pap smear was 46 (range: 17-74) years for the IBD group and matched controls (range: 19-72 years). There were more Caucasian subjects than other ethnicities in the IBD patient group (P = 0.025), as well as fewer abortions (P = 0.008), but there was no significant difference regarding marital status. Eighteen percent of IBD patients had abnormal Pap smears compared to 5% of controls (P = 0.004). Subgroup analysis of the IBD patients revealed no significant differences between CD and UC patients in age, ethnicity, marital status, number of abortions, disease severity, family history of IBD, or disease duration. No significant difference was observed in the number of abnormal Pap smears or the use of immunosuppressive medications between CD and UC patients (P = 0.793). No definitive observation could be made regarding HPV status, as this was not routinely investigated during the timeframe of our study.CONCLUSION: Diagnosis of IBD in women is related to an increased risk of abnormal Pap smear, while type of IBD and exposure to immunosuppressive medications are not. This has significant implications for

  18. Sobrevida en pacientes con cáncer de cervix

    Directory of Open Access Journals (Sweden)

    Adolfo Ortiz-Barboza

    2005-10-01

    Full Text Available Objetivo: Estimar la probabilidad acumulada de sobrevida a cinco años, de una cohorte de pacientes diagnosticadas con cáncer de cervix durante 1999. Metodología: Para el análisis de la información se utilizaron distribuciones de frecuencia con valores absolutos y porcentuales, medidas de tendencia central y de dispersión. La relación entre variables cualitativas se efectuó mediante la prueba estadística de Chi cuadrado (X2. El nivel de significancia se fijó en p Objective: To estimate 5 year calculate the five-year cumulative survival probability of a patient cohort diagnosed with interine cervical cancer during 1999. Methods: To analyse of the information, we used frequency distributions with absolute values as well as percentages, measures of central tendency as well as of variance. The relation ship between cualitative variables was done by means of a chi square statistical test. A p value less or equal to 0.05, was significant. The cumulative survival probability was done using the Kaplan-Meier method. The comparison between the survival times was done using a logarithmic range test. The level of significance was again fixed at less or equal to 0.05. Results: A total of 778 cases were studied at the end of the study period, 91 patients had died. The 5 year, cumulative survival probability for the whole population was 88.3%. The patients in which carcinoma in situ was detected at the time of diagnosis, showed a cumulative survival probability of 98.8% while those with invasive carcinoma had a value of 68.3%. This difference was statistically significant. The cumulative survival probability for the patients diagnosed with squamous cell carcinoma was 89% while those with adenocarcinoma had a value of 80%. This difference did not fall within the range of statistical significance, though. Conclusions: If we compare our results with similar investigations done elsewhere, we can see that our values are superior to those seen in countries

  19. 宫颈炎症患者阴道局部免疫状态与疾病发生的相关性%Relevance between part of vagina's immune status and occurrence of disease of patients with cervicitis

    Institute of Scientific and Technical Information of China (English)

    黄益于; 郭芙蓉

    2015-01-01

    目的:探讨分析宫颈炎症患者阴道局部免疫状态与疾病发生的相关性。方法选取我院2013年4月~2014年4月收治的250例宫颈炎症患者,分为淋病奈瑟菌宫颈炎(NG,50例)、人型支原体宫颈炎(MH,50例)、宫颈糜烂患者(EC,50例)、沙眼衣原体宫颈炎(CT,50例)、解脲支原体宫颈炎(UU,50例)和250例正常妇女作为对照组,对所有患者进行阴道灌洗,并应用酶联免疫吸附试验检测洗液中肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)的水平,其中给予观察组宫颈炎康栓配合射频治疗该病,对照组采用常规射频治疗,对比其疗效和局部免疫状态与疾病发生的相关性。结果阴道灌洗液TNF-α水平,除宫颈糜烂患者(EC)组之外,其余各组均高于对照组,组间比较差异有统计学意义(P<0.01)。阴道灌洗液IL-8水平,NG组为(336±35)ng/L,MH组为(369±33)ng/L,CT组为(363±31)ng/L,三组和对照组相比,为(314±33)ng/L,(343±35)ng/L及(337±36)ng/L,组间比较差异有统计学意义(P<0.05)。UU组和EC组与对照组比较差异无统计学意义(P>0.05)。宫颈炎组治疗总有效率优于对照组(P<0.05)。结论阴道局部免疫状态在各种宫颈炎症的发病机制中发挥了重要作用,尤其在与疾病发生的相关性中IL-8在人型支原体宫颈炎、沙眼衣原体宫颈炎及淋病奈瑟菌宫颈炎中有更重要的作用。%ObjectiveTo explore and analyze the relevance between part of vagina's immune status and occurrence of disease of patients with cervicitis.Methods250 patients with cervicitis who were admitted to our hospital from April 2013 to April 2014 were allocated into Neisseria gonorrhoeae cervicitis group (NG, 50 patients), Mycoplasma hominis cervicitis group (MH, 50 cases), erosion of cervix group (EC, 50 cases), cervicitis of chlamydia trachomatis group (CT

  20. The clinical value of squamous cell carcinoma antigen in cancer of the uterine cervix

    NARCIS (Netherlands)

    de Bruijn, HWA; Duk, JM; van der Zee, AGJ; Pras, E; Willemse, PHB; Hollema, H; Mourits, MJE; de Vries, EGE; Aalders, JG; Boonstra, J.

    1998-01-01

    A review is given of the clinical use and interpretation of serum tumor marker levels during the treatment of patients with cancer of the uterine cervix, Pretreatment serum squamous cell carcinoma (SCC) antigen provides a new prognostic factor in early stage squamous cell carcinoma of the uterine ce

  1. Concurrent chemo- and radiotherapy in patients with locally advanced carcinoma of the cervix

    NARCIS (Netherlands)

    Pras, E; Willemse, PHB; Hollema, H; Heesters, MAAM; Szabo, BG; deBruijn, HWA; Aalders, JG; deVries, EGE; Boonstra, J.

    1996-01-01

    Background: The feasibility of concurrent chemotherapy and radiotherapy for advanced primary carcinoma of the cervix was evaluated and the results were compared to historical controls. Patients and methods: In a single institution study, patients (n = 74) with primary cervical carcinoma received 3 c

  2. Concurrent chemo- and radiotherapy in patients with locally advanced carcinoma of the cervix

    NARCIS (Netherlands)

    Pras, E; Willemse, P H; Boonstra, H; Hollema, H; Heesters, M A; Szabó, B G; de Bruijn, H W; Aalders, J G; de Vries, E G

    1996-01-01

    BACKGROUND: The feasibility of concurrent chemotherapy and radiotherapy for advanced primary carcinoma of the cervix was evaluated and the results were compared to historical controls. PATIENTS AND METHODS: In a single institution study, patients (n = 74) with primary cervical carcinoma received 3 c

  3. The Reserve Cell in the Uterine Cervix: aspects of development, differentiation and diagnosis

    NARCIS (Netherlands)

    J.E. van Muyden-Martens (Jolise)

    2008-01-01

    textabstractCarcinoma of the uterine cervix is worldwide the second most common cancer in women1. It has been approximately 150 years since the first description of uterine cervical carcinoma, a century since the description of its precursor lesions2, and half a century since the introduction of the

  4. PSYCHOSEXUAL FUNCTIONING AFTER TREATMENT FOR CANCER OF THE CERVIX - A COMPARATIVE AND LONGITUDINAL-STUDY

    NARCIS (Netherlands)

    SCHULTZ, WCMW; VANDEWIEL, HBM; BOUMA, J

    1991-01-01

    Twenty-six couples participated in a 2-year longitudinal study of sexual functioning before and after treatment for carcinoma of the cervix. Sexual functioning was measured on admission prior to their treatment and at 6, 12 and 24 months follow-up and made operational in terms of current sexual beha

  5. A rare case of minimal deviation adenocarcinoma of the uterine cervix in a renal transplant recipient.

    LENUS (Irish Health Repository)

    Fanning, D M

    2009-02-03

    INTRODUCTION: We report the first described case of minimal deviation adenocarcinoma of the uterine cervix in the setting of a female renal cadaveric transplant recipient. MATERIALS AND METHODS: A retrospective review of this clinical case was performed. CONCLUSION: This rare cancer represents only about 1% of all cervical adenocarcinoma.

  6. A rare case of minimal deviation adenocarcinoma of the uterine cervix in a renal transplant recipient.

    LENUS (Irish Health Repository)

    Fanning, D M

    2012-02-01

    INTRODUCTION: We report the first described case of minimal deviation adenocarcinoma of the uterine cervix in the setting of a female renal cadaveric transplant recipient. MATERIALS AND METHODS: A retrospective review of this clinical case was performed. CONCLUSION: This rare cancer represents only about 1% of all cervical adenocarcinoma.

  7. Surgical excision of lung metastases from squamous carcinoma of the cervix. A report of 2 cases

    Energy Technology Data Exchange (ETDEWEB)

    De Moor, N.G.; Berry, A.V.; Nissenbaum, M.M. (University of the Witwatersrand, Johannesburg (South Africa))

    1983-01-01

    These 2 case reports serve to emphasize two important points concerning carcinoma of the cervix: (i) blood-borne metastases are now frequently encountered in this disease; and (ii) in selected cases surgical excision of a secondary deposit in the lung is the treatment of choice and may even result in cure.

  8. Surgical excision of lung metastases from squamous carcinoma of the cervix. A report of 2 cases.

    Science.gov (United States)

    de Moor, N G; Berry, A V; Nissenbaum, M M

    1983-01-01

    These 2 case reports serve to emphasize two important points concerning carcinoma of the cervix: (i) blood-borne metastases are now frequently encountered in this disease; and (ii) in selected cases surgical excision of a secondary deposit in the lung is the treatment of choice and may even result in cure.

  9. Distensibility and pain of the uterine cervix evaluated by novel techniques

    DEFF Research Database (Denmark)

    Gregersen, Hans; Hee, Lene; Liao, Donghua;

    2016-01-01

    The article serves to review the literature on the human uterine cervix based on a new distension technology named Functional Luminal Imaging Probe (FLIP). This technology was originally developed to study the biomechanical competence of the gastro-esophageal junction where it provides a geometri...

  10. A Rare Presentation of Lymphoma of the Cervix with Cross-Sectional Imaging Correlation

    Directory of Open Access Journals (Sweden)

    Brinda Rao Korivi

    2014-01-01

    Full Text Available Non-Hodgkin’s lymphoma of the cervix is an extremely uncommon entity, with no standard established treatment protocol. A 43-year-old asymptomatic female with a history of dual hit blastic B-cell lymphoma/leukemia in complete remission presented with an incidental cervical mass, which was initially felt to represent a cervical fibroid on computed tomography (CT. It was further evaluated with ultrasound, biopsy, and positron emission tomography-computed tomography (PET-CT, which demonstrated a growing biopsy-proven lymphomatous mass and new humeral head lesion. The patient was started on chemotherapy to control the newly diagnosed humeral head lesion, which then regressed. She then underwent radiation to the cervix with significant improvement in the cervical lymphoma. A review of cross-sectional imaging findings of lymphoma of the cervix is provided, including how to differentiate it from other more common diseases of the cervix. Clinical awareness of rare cervical masses such as lymphoma is very important in order to achieve timely diagnosis and appropriate treatment.

  11. Papillary squamotransitional cell carcinoma of the uterine cervix: A histomorphological and immunohistochemical study of nine cases

    Directory of Open Access Journals (Sweden)

    Mani Anand

    2013-01-01

    Full Text Available Background: Papillary squamotransitional cell carcinoma (PSCC is a distinctive subcategory of squamous cell carcinoma of the uterine cervix. It has a propensity for local recurrence and late metastasis. Histologically, it can be misinterpreted as transitional cell carcinoma, or other papillary lesions of the cervix including squamous papilloma, verrucous carcinoma or cervical intraepithelial neoplasia grade 3 with papillary configuration. Materials and Methods: Nine cases of PSCC of the uterine cervix were diagnosed on a cervical biopsy specimen on routine hematoxylin and eosin (H and E stained sections. Their clinic-morphological features were analyzed. The cases were further evaluated immunohistochemically by cytokeratin 7 (CK7, cytokeratin 20 (CK20, p53 and Ki-67. Results: The patients ranged in age from 35 years to 75 years; with abnormal uterine bleeding being the most common clinical presentation. All the cases showed papillary architecture with fibrovascular cores lined by multilayered atypical epithelium. Three cell types were observed: Clear, intermediate and basaloid. Stromal invasion was seen in five cases, whereas in the remaining four cases, the biopsy specimen was too superficial to definitely assess invasion. Immunohistochemically, eight cases were CK7 + /CK20 - and one case was CK7 - /CK20 - . All nine cases showed nuclear accumulation of mutant p53. Moderate and high proliferative activity was observed in two and seven cases, respectively. Five of patients for whom follow-up information was available underwent radical hysterectomy and two of them were disease free 18 months following treatment. Conclusion: PSCC of the uterine cervix are a clinicomorphologically distinct group of cervical lesions that display a morphologic spectrum. They are potentially aggressive malignant tumors that should be distinguished from transitional cell carcinoma and other papillary lesions of the uterine cervix.

  12. Progesterone Downregulates Oestrogen-Induced Expression of CFTR and SLC26A6 Proteins and mRNA in Rats’ Uteri

    Directory of Open Access Journals (Sweden)

    K. Gholami

    2012-01-01

    Full Text Available Under progesterone (P dominance, fluid loss assists uterine closure which is associated with pH reduction. We hypothesize that P inhibits uterine fluid secretion and HCO3- transport. Aim. to investigate the expression of Cystic Fibrosis Transmembrane Regulator (CFTR and Cl−/HCO3- exchanger (SLC26A6 under P effect. Method. Uteri from ovariectomized steroid replaced and intact rats at different stages of oestrous cycle were analyzed for changes in protein and mRNA expressions. Results. P inhibits CFTR and SLC26A6 proteins and mRNA expression while oestrogen (E causes vice versa. E treatment followed by P causes a reduction in these transporters’ mRNA and protein. Similar changes occur throughout the oestrous cycle; that is, CFTR mRNA expression was high at proestrus while SLC26A6 mRNA and protein expressions were increased at proestrus and estrus. At diestrus, however, the expression of these transporters’ protein and mRNA was reduced. Conclusion. Inhibition of CFTR and SLC26A6 expressions may explain the reduced fluid volume and pH under P-mediated effect.

  13. Case Report of Diffuse Large B Cell Lymphoma of Uterine Cervix Treated at a Semiurban Cancer Centre in North India

    Science.gov (United States)

    Sridhar, Epari

    2016-01-01

    Lymphoma of the uterine cervix is very rare. We report a case of diffuse large B cell lymphoma (DLBCL) involving the uterine cervix treated at a newly commissioned semiurban cancer centre in north India in 2015. Data for this study was obtained from the hospital electronic medical records and the patient's case file. We also reviewed published case reports of uterine and cervical lymphoma involving forty-one patients. We treated a case of stage IV DLBCL cervix with six cycles of R-CHOP (rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisolone) and intrathecal methotrexate followed by consolidation with radiotherapy. The patient showed complete response to chemotherapy. We conclude that, in advanced stage lymphoma involving uterus and cervix, combination of chemotherapy and radiotherapy is effective in short term. PMID:27597906

  14. Case Report of Diffuse Large B Cell Lymphoma of Uterine Cervix Treated at a Semiurban Cancer Centre in North India

    Directory of Open Access Journals (Sweden)

    Vibhor Sharma

    2016-01-01

    Full Text Available Lymphoma of the uterine cervix is very rare. We report a case of diffuse large B cell lymphoma (DLBCL involving the uterine cervix treated at a newly commissioned semiurban cancer centre in north India in 2015. Data for this study was obtained from the hospital electronic medical records and the patient’s case file. We also reviewed published case reports of uterine and cervical lymphoma involving forty-one patients. We treated a case of stage IV DLBCL cervix with six cycles of R-CHOP (rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisolone and intrathecal methotrexate followed by consolidation with radiotherapy. The patient showed complete response to chemotherapy. We conclude that, in advanced stage lymphoma involving uterus and cervix, combination of chemotherapy and radiotherapy is effective in short term.

  15. PECULIARITIES OF CHANGES ОF IMMUNOREGULATORY SYSTEM OF WOMEN WITH BACKGROUNG PATHOLOGY OF UTERINE CERVIX (NA

    Directory of Open Access Journals (Sweden)

    N.B. Zakharova

    2009-03-01

    Full Text Available A comparative research of cytokines IL-1b, IL-4, IL-6, IL-8, INF-/, TNF-a and its concentration in cellular material suspension on surface of pseudo erosion of uterine cervix was carried out. It was clarified that inefficient activation of local protective factors of immune system plays the main role in clinical behavior of pseudo erosion of uterine cervix. Imbalance of cytokines output to pro-inflammatory factors was observed.

  16. Loss of progesterone receptor-mediated actions induce preterm cellular and structural remodeling of the cervix and premature birth.

    Directory of Open Access Journals (Sweden)

    Steven M Yellon

    Full Text Available A decline in serum progesterone or antagonism of progesterone receptor function results in preterm labor and birth. Whether characteristics of premature remodeling of the cervix after antiprogestins or ovariectomy are similar to that at term was the focus of the present study. Groups of pregnant rats were treated with vehicle, a progesterone receptor antagonist (onapristone or mifepristone, or ovariectomized on day 17 postbreeding. As expected, controls given vehicle delivered at term while rats delivered preterm after progesterone receptor antagonist treatment or ovariectomy. Similar to the cervix before term, the preterm cervix of progesterone receptor antagonist-treated rats was characterized by reduced cell nuclei density, decreased collagen content and structure, as well as a greater presence of macrophages per unit area. Thus, loss of nuclear progesterone receptor-mediated actions promoted structural remodeling of the cervix, increased census of resident macrophages, and preterm birth much like that found in the cervix at term. In contrast to the progesterone receptor antagonist-induced advance in characteristics associated with remodeling, ovariectomy-induced loss of systemic progesterone did not affect hypertrophy, extracellular collagen, or macrophage numbers in the cervix. Thus, the structure and macrophage census in the cervix appear sufficient for premature ripening and birth to occur well before term. With progesterone receptors predominantly localized on cells other than macrophages, the findings suggest that interactions between cells may facilitate the loss of progesterone receptor-mediated actions as part of a final common mechanism that remodels the cervix in certain etiologies of preterm and with parturition at term.

  17. Vaginal progesterone in women with an asymptomatic sonographic short cervix in the midtrimester decreases preterm delivery and neonatal morbidity

    DEFF Research Database (Denmark)

    Romero, Roberto; Nicolaides, Kypros; Conde-Agudelo, Agustin;

    2012-01-01

    To determine whether the use of vaginal progesterone in asymptomatic women with a sonographic short cervix (≤ 25 mm) in the midtrimester reduces the risk of preterm birth and improves neonatal morbidity and mortality.......To determine whether the use of vaginal progesterone in asymptomatic women with a sonographic short cervix (≤ 25 mm) in the midtrimester reduces the risk of preterm birth and improves neonatal morbidity and mortality....

  18. Arabin cervical pessary to prevent preterm birth in twin pregnancies with short cervix.

    Science.gov (United States)

    Di Tommaso, Mariarosaria; Seravalli, Viola; Arduino, Silvana; Bossotti, Carlotta; Sisti, Giovanni; Todros, Tullia

    2016-08-01

    A retrospective study was conducted to evaluate the effect of Arabin cervical pessary in twin pregnancies with cervical length (CL)  <25 mm between 21 and 31 weeks. Forty patients receiving pessary were matched with 40 controls without pessary. They were matched for gestational age (GA) at admission and CL. GA at delivery, delivery before 36, 34 and 32 weeks, latency between detection of short cervix and delivery, and duration of hospital admission were compared between groups. Women with the pessary delivered at higher GA compared to controls (35 vs. 33 weeks, p = 0.02). Cervical pessary significantly reduced the incidence of delivery  <36 and  < 34 weeks (p < 0.05), but not before 32 weeks. Interval between detection of short cervix and delivery was longer in the pessary group and duration of hospital admission was shorter (p = 0.03) compared to women without pessary.

  19. Mullerian adenosarcoma (heterologous) of the cervix with sarcomatous overgrowth: a case report with review of literature

    Science.gov (United States)

    Nijhawan, Raje; Aggarwal, Neelam; Sikka, Pooja

    2010-01-01

    Mullerian adenosarcoma is a rare biphasic malignant neoplasm of the cervix characterized by an admixture of benign epithelial elements and a malignant sarcomatous stromal component, which may be either homologous or heterologous. An aggressive variant of adenosarcoma, mullerian adenosarcoma with sarcomatous overgrowth (MASO) is extremely rare, with only two such cases being reported in the English literature to date. In this report we present a case of MASO of uterine cervix with heterologous elements in a 15-year-old unmarried girl presenting with foul smelling menstrual bleeding and passage of fleshy masses. Because MASO with heterologous elements seems to appear at the earliest stages of reproductive lifespan in women, and have an uncertain malignant potential, gynecologists and pathologists should be aware and think about the possibility of this tumor. PMID:20613904

  20. A Case of Secondary Infertility Due to Retention of Fetal Bones in Cervix

    Directory of Open Access Journals (Sweden)

    Alev Ozer

    2014-12-01

    Full Text Available A case of secondary infertility related to prolonged retention of fetal bones in the cervix is presented. A 34-year-old nulliparous woman was admitted to the hospital with chronic pelvic pain, dyspareunia, and 12%u2013year-long secondary infertility following an induced abortion due to fetal demise. Transvaginal ultrasonography (USG revealed a linear echogenic area around the posterior cervical wall. An hysteroscopic examination confirmed the existence of an irregular calcified mass embedded in the cervix. The mass turned out to be conglomerated fetal bone fragments which probably acted as an intrauterine device. After removal of the cervical mass, the patient conceived spontaneously within four months. The present case report emphasizes the significance of a detailed history and a thorough evaluation by transvaginal USG in the case of secondary infertility following an induced or spontaneous abortion.

  1. Effect of spasmoanalgetic Dolantin on the electromyographic activity of the cervix during labour.

    Science.gov (United States)

    Pajntar, M; Rudel, D

    1992-01-01

    The influence of spasmoanalgetic Dolantin on electrical activity of the smooth musculature of the cervix during labour was studied in 50 primiparous women after induction of labour. The highest electrical activity was measured at the time of uterine contraction (AC) and between contractions (BC). The basic pattern of Dolantin-produced changes in muscular contractions in the cervix observed via EMG activity is that of the EMG activity diminishing with contractions of the uterine corpus and even more so in the period between contractions. Dolantin administered during the latent phase of induced labour did not accelerate cervical dilatation either in the latent or in the active phase of amniotomy- and Oxytocin-induced labour.

  2. In vivo high-resolution magnetic resonance elastography of the uterine corpus and cervix

    Energy Technology Data Exchange (ETDEWEB)

    Jiang, Xuyuan [The First Affiliated Hospital of China Medical University, Department of Radiology, Shenyang (China); Asbach, Patrick; Streitberger, Kaspar-Josche; Hamm, Bernd; Sack, Ingolf; Guo, Jing [Charite - Universitaetsmedizin Berlin, Department of Radiology, Berlin (Germany); Thomas, Anke [Charite - Universitaetsmedizin Berlin, Departments of Gynecology and Obstetrics, Berlin (Germany); Braun, Juergen [Charite - Universitaetsmedizin Berlin, Department of Medical Informatics, Berlin (Germany)

    2014-12-15

    To apply 3D multifrequency MR elastography (3DMMRE) to the uterus and analyse the viscoelasticity of the uterine tissue in healthy volunteers considering individual variations and variations over the menstrual cycle. Sixteen healthy volunteers participated in the study, one of whom was examined 12 times over two menstrual cycles. Pelvic 3DMMRE was performed on a 1.5-T scanner with seven vibration frequencies (30-60 Hz) using a piezoelectric driver. Two mechanical parameter maps were obtained corresponding to the magnitude (vertical stroke G* vertical stroke) and the phase angle (φ) of the complex shear modulus. On average, the uterine corpus had higher elasticity, but similar viscosity compared with the cervix, reflected by vertical stroke G* vertical stroke {sub uterine} {sub corpus} = 2.58 ± 0.52 kPa vs. vertical stroke G* vertical stroke {sub cervix} = 2.00 ± 0.34 kPa (p < 0.0001) and φ {sub uterine} {sub corpus} = 0.54 ± 0.08, φ {sub cervix} = 0.57 ± 0.12 (p = 0.428). With 2.23 ± 0.26 kPa, vertical stroke G* vertical stroke of the myometrium was lower in the secretory phase (SP) compared with that of the proliferative phase (PP, vertical stroke G* vertical stroke = 3.01 ± 0.26 kPa). For the endometrium, the value of vertical stroke G* vertical stroke in SP was 68 % lower than during PP (PP, vertical stroke G* vertical stroke = 3.34 ± 0.42 kPa; SP, vertical stroke G* vertical stroke = 1.97 ± 0.34 kPa; p = 0.0061). 3DMMRE produces high-resolution mechanical parameter maps of the uterus and cervix and shows sensitivity to structural and functional changes of the endometrium and myometrium during the menstrual cycle. (orig.)

  3. Radiotherapy for carcinoma of the uterine cervix with short uterine cavity

    Energy Technology Data Exchange (ETDEWEB)

    Tsuchida, Emiko; Sugita, Tadashi; Matsumoto, Yasuo; Sasamoto, Ryuta; Sakai, Kunio [Niigata Univ. (Japan). School of Medicine; Sueyama, Hiroo; Yamanoi, Tadayoshi; Ito, Takeshi; Umetsu, Hisao

    2001-11-01

    We have reviewed 11 patients treated with radiotherapy for carcinoma of the uterine cervix with short uterine cavity less than 3 cm. They consisted of 5 patients with stump cancer and 6 with atrophic uterus. Two patients with stump cancer had local failure and died of tumor progression. The 5-year cause-specific survival was 82%. Rectal and bladder injuries occurred in 5 and 2 patients, respectively. These complications were observed frequently in the patients with atrophic uterus. (author)

  4. Concomitant mucin-producing tumors of ovary and adenocarcinoma of cervix: a case report

    Directory of Open Access Journals (Sweden)

    Mousavi A

    2013-05-01

    Full Text Available Background: Ovarian mucinous borderline tumors are divided into two morphologic groups: endocervical-like and intestinal type. Most endocervical adenocarcinomas exhibit mucinous and/or endometrioid differentiation, they infrequently metastasize to the ovaries but may simulate primary ovarian tumors (both atypical proliferative or borderline and carcinoma. In patients with mucinous adenocarcinoma in the abdominal cavity, caution should be exercised in interpreting the possible primary site of the tumor on the basis of the immunohistochemical profiles. The presence of human papillomavirus (HPV DNA is assessed to determine whether the ovarian neoplasms were metastases or primary independent neoplasm. Approximately 90% of endocervical adenocarcinomas are related to high-risk human papillomavirus (hr-HPV with the remainder being unrelated to HPV. Both types metastasize to the ovaries very infrequently. Ovarian endocervical-type (mullerian mucinous tumors and tumors composed of a mixture of endocervical-type mucinous, serous endometrioid, squamous, and indifferent cells with abundant eosinophilic cytoplasm reported to date have been primarily limited to borderline and micro invasive types. We report a-36-yr old woman with adenocarcinomas of uterine cervix who also had ovarian mucinous borderline tumor.Case presentation: The patient presented with abnormal uterine bleeding and lower abdominal pain. She had a history of uterine cervix polyps. Pelvic ultrasound showed a right adnexal mass and a large cervical size. Histological diagnosis in uterine cervix biopsy revealed adenocarcinoma of cervix. Radical hysterectomy type III with bilateral salpingo-oophorectomy was performed. Histological finding in adnexal mass revealed borderline mucinous tissue of ovarian tumor. Testing for HPV DNA in the tumoral tissue was negative. This confirms that the ovarian tumor is not metastatic from endocervical adenocarcinoma. Conclusion: We conclude that in a patient with

  5. A Case of Secondary Infertility Due to Retention of Fetal Bones in Cervix

    OpenAIRE

    Alev Ozer

    2014-01-01

    A case of secondary infertility related to prolonged retention of fetal bones in the cervix is presented. A 34-year-old nulliparous woman was admitted to the hospital with chronic pelvic pain, dyspareunia, and 12%u2013year-long secondary infertility following an induced abortion due to fetal demise. Transvaginal ultrasonography (USG) revealed a linear echogenic area around the posterior cervical wall. An hysteroscopic examination confirmed the existence of an irregular calcified mass embedded...

  6. Chlamydial infection of the cervix in contacts of men with nongonococcal urethritis.

    OpenAIRE

    Tait, I A; Rees, E; Hobson, D.; Byng, R E; Tweedie, M C

    1980-01-01

    An investigation of chlamydial infection in sexual contacts of patients with nongonococcal urethritis (NGU) was carried out to determine the clinical signs of infection in the cervix, and their response to chemotherapy, and the incidence of cervical infection in the presence of ectopy and oral contraception. In 202 consecutive female contacts of NGU the isolation rate of Chlamydia trachomatis was 35%. Hypertrophic ectopy and endocervical mucopus were present in 19% and 37% of chlamydia-positi...

  7. Titrated oral misoprostol solution compared with oxytocin for induction of labor in women with unfavorable cervix

    Directory of Open Access Journals (Sweden)

    Akrami M

    2011-10-01

    Full Text Available "nBackground: Uterine contractions and an appropriate cervix are two important factors in labor contributing to good pregnancy outcomes. Oxytocin and prostaglandins, such as misoprostol, are used for the induction of labor. Misoprostol is used for cervical ripening and labor induction. The aim of this trial was to compare the efficacy and safety of titrated oral misoprostol solution with oxytocin for labor induction in pregnant women with an unfavorable cervix."n "nMethods: In this randomized double-blind clinical trial, 140 women with a gestational age of 34-42 weeks and an unfavorable cervix were recruited. The participants had an indication for labor induction and had been referred to the Women's Hospital in Tehran, Iran between January 2010 and January 2011. The participants were randomly assigned to receive 20 µg/hour titrated oral misoprostol plus intravenous placebo or 6 mU/min oxytocin plus oral placebo. In case contractions were inadequate, the drug doses were gradually increased. Pharmacological complications, the mean interval from the start of induction till vaginal delivery and delivery type were monitored and analyzed in both groups."n "nResults: The mean interval from the start of induction till vaginal delivery in misoprostol group was shorter than the oxytocin group (11.07±3.42 vs. 14.87±3.21 hours, P=0.001. The frequency of pharmacological complications and vaginal or cesarean deliveries were similar between the two groups (P>0.05."n "nConclusion: Use of titrated oral misoprostol is a safe and effective method for labor induction in pregnant women with unfavorable cervix. Misoprostol is associated with a shorter interval from induction to vaginal delivery than oxytocin.

  8. Relaxant effect of the essential oil of Croton nepetifolius on ovine cervix

    Directory of Open Access Journals (Sweden)

    Alexsandra F. Pereira

    2012-06-01

    Full Text Available We investigated the in vitro effect of the essential oil of Croton nepetifolius Baill., Euphorbiaceae (EOCN, on spontaneous or induced contractions of circular and longitudinal muscles from the ovine cervix during the luteal phase of the estrous cycle. The relaxant effect of EOCN was expressed as a percentage of the contraction recorded before the addition of the oil and calculated relative to the preparations exposed only to the vehicle. The IC50 (concentration of oil required to produce a 50% maximal reduction in muscle contraction for relaxation of spontaneous contractions in circular and longitudinal muscles was significantly lower than the IC50 for blockade of K+-induced contraction (27.19 µg mL-1 versus 262.72 µg mL-1 and 40.92 µg mL-1 versus 222.47 µg mL-1, respectively. Interestingly, there was a high degree of selectivity in the action of EOCN on cervix layers concerning the inhibition of acetylcholine-induced contraction in circular (IC50 277.10 µg mL-1 and longitudinal (IC50 52.56 µg mL-1 muscles. In conclusion, EOCN is able to relax ovine cervix during the luteal phase. This work opens the perspective of applying EOCN in ovine embryo transfer.

  9. Radiation dose delivery verification in the treatment of carcinoma-cervix

    Energy Technology Data Exchange (ETDEWEB)

    Shrotriya, D., E-mail: shrotriya2007@gmail.com; Srivastava, R. N. L. [Department of Radiotherapy, J.K. Cancer Institute Kanpur-208019 (India); Kumar, S. [Department of Physics, Christ Church College, Kanpur-208001 (India)

    2015-06-24

    The accurate dose delivery to the clinical target volume in radiotherapy can be affected by various pelvic tissues heterogeneities. An in-house heterogeneous woman pelvic phantom was designed and used to verify the consistency and computational capability of treatment planning system of radiation dose delivery in the treatment of cancer cervix. Oncentra 3D-TPS with collapsed cone convolution (CCC) dose calculation algorithm was used to generate AP/PA and box field technique plan. the radiation dose was delivered by Primus Linac (Siemens make) employing high energy 15 MV photon beam by isocenter technique. A PTW make, 0.125cc ionization chamber was used for direct measurements at various reference points in cervix, bladder and rectum. The study revealed that maximum variation between computed and measured dose at cervix reference point was 1% in both the techniques and 3% and 4% variation in AP/PA field and 5% and 4.5% in box technique at bladder and rectum points respectively.

  10. Radiation dose delivery verification in the treatment of carcinoma-cervix

    Science.gov (United States)

    Shrotriya, D.; Kumar, S.; Srivastava, R. N. L.

    2015-06-01

    The accurate dose delivery to the clinical target volume in radiotherapy can be affected by various pelvic tissues heterogeneities. An in-house heterogeneous woman pelvic phantom was designed and used to verify the consistency and computational capability of treatment planning system of radiation dose delivery in the treatment of cancer cervix. Oncentra 3D-TPS with collapsed cone convolution (CCC) dose calculation algorithm was used to generate AP/PA and box field technique plan. the radiation dose was delivered by Primus Linac (Siemens make) employing high energy 15 MV photon beam by isocenter technique. A PTW make, 0.125cc ionization chamber was used for direct measurements at various reference points in cervix, bladder and rectum. The study revealed that maximum variation between computed and measured dose at cervix reference point was 1% in both the techniques and 3% and 4% variation in AP/PA field and 5% and 4.5% in box technique at bladder and rectum points respectively.

  11. Assessment of improved organ at risk sparing for advanced cervix carcinoma utilizing precision radiotherapy techniques

    Energy Technology Data Exchange (ETDEWEB)

    Georg, D.; Georg, P.; Hillbrand, M.; Poetter, R.; Mock, U. [Dept. of Radiotherapy, Medical Univ. AKH, Vienna (Austria)

    2008-11-15

    Purpose: to evaluate the potential benefit of proton therapy and photon based intensity-modulated radiotherapy in comparison to 3-D conformal photon radiotherapy (3D-CRT) in locally advanced cervix cancer. Patients and methods: in five patients with advanced cervix cancer 3D-CRT (four-field box) was compared with intensity modulated photon (IMXT) and proton therapy (IMPT) as well as proton beam therapy (PT) based on passive scattering. Planning target volumes (PTVs) included primary tumor and pelvic and para-aortic lymph nodes. Dose-volume histograms (DVHs) were analyzed for the PTV and various organs at risk (OARs) (rectal wall, bladder, small bowel, colon, femoral heads, and kidneys). In addition dose conformity, dose inhomogeneity and overall volumes of 50% isodoses were assessed. Results: all plans were comparable concerning PTV parameters. Large differences between photon and proton techniques were seen in volumes of the 50% isodoses and conformity indices. DVH for colon and small bowel were significantly improved with PT and IMPT compared to IMXT, with D{sub mean} reductions of 50-80%. Doses to kidneys and femoral heads could also be substantially reduced with PT and IMPT. Sparing of rectum and bladder was superior with protons as well but less pronounced. Conclusion: proton beam RT has significant potential to improve treatment related side effects in the bowel compared to photon beam RT in patients with advanced cervix carcinoma. (orig.)

  12. Remodeling of the Cervix and Parturition in Mice Lacking the Progesterone Receptor B Isoform1

    Science.gov (United States)

    Yellon, Steven M.; Oshiro, Bryan T.; Chhaya, Tejas Y.; Lechuga, Thomas J.; Dias, Rejane M.; Burns, Alexandra E.; Force, Lindsey; Apostolakis, Ede M.

    2011-01-01

    Withdrawal of progestational support for pregnancy is part of the final common pathways for parturition, but the role of nuclear progesterone receptor (PGR) isoforms in this process is not known. To determine if the PGR-B isoform participates in cervical remodeling at term, cervices were obtained from mice lacking PGR-B (PGR-BKO) and from wild-type (WT) controls before or after birth. PGR-BKO mice gave birth to viable pups at the same time as WT controls during the early morning of Day 19 postbreeding. Morphological analyses indicated that by the day before birth, cervices from PGR-BKO and WT mice had increased in size, with fewer cell nuclei/area as well as diminished collagen content and structure, as evidenced by optical density of picrosirius red-stained sections, compared to cervices from nonpregnant mice. Moreover, increased numbers of resident macrophages, but not neutrophils, were found in the prepartum cervix of PGR-BKO compared to nonpregnant mice, parallel to findings in WT mice. These results suggest that PGR-B does not contribute to the growth or degradation of the extracellular matrix or proinflammatory processes associated with recruitment of macrophages in the cervix leading up to birth. Rather, other receptors may contribute to the progesterone-dependent mechanism that promotes remodeling of the cervix during pregnancy and in the proinflammatory process associated with ripening before parturition. PMID:21613631

  13. COMPARISION AND CORRELATION OF PAP SMEAR WITH COLPOSCOPY AND HISTOPATHIOLOGY IN EVALUATION OF CERVIX

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    Zainab S

    2015-07-01

    Full Text Available AIMS AND OBJECTIVES : Correlate pap smear findings wi th colposcopic findings, To localize the lesion by colposcopy and obtain biopsy and to provide appropriate treatment wherever possible. MATERIAL AND METHODS : This was a prospective comparative study of 104 patients who attended the Gynecology OPD of KIMS f rom may2012 to may 2014. INCLUSION CRITERIA : 1. Women of age between 20 - 65 years. 2. Women with symptoms like vaginal discharge, post coital bleeding, postmenopausal bleeding, intermenstrual bleeding and persistent leucorrhoea . 3. Women with normal looking cervix but symptomatic. EXCLUSION CRITERIA : 1. Women with bleeding at the time of examination . 2. Women with frank lesions . 3. Women with clinical evidence of acute pelvic infection . 4. Women who was previously treated for carcinoma cervix . 5. Pregnant wome n. RESULTS : Sensitivity of pap smear was found to be very low which was 31.25% compared to its specificity which was 94.44%. Which means pap smear shows higher no. of false negative smears Colposcopy showed a high sensitivity 96.57% and a good specificity 88.55% compared to pap smear . CONCLUSIOS: It is evident that colposcopy is definitely more sensitive and accurate than pap smear. By combining pap smear with colposcopy, we can maximize the sensitivity and specificity of cancer cervix screening.

  14. Quercetin Induces Dose-Dependent Differential Morphological and Proliferative Changes in Rat Uteri in the Presence and in the Absence of Estrogen.

    Science.gov (United States)

    Shahzad, Huma; Giribabu, Nelli; Sekaran, Muniandy; Salleh, Naguib

    2015-12-01

    Quercetin could have profound effects on uterine morphology and proliferation, which are known to be influenced by estrogen. This study investigated the effect of quercetin on these uterine parameters in the presence and in the absence of estrogen. Ovariectomized adult female rats received peanut oil, quercetin (10, 50, and 100 mg/kg/day), estrogen, or estrogen+quercetin (10, 50, or 100 mg/kg/day) treatment for 7 consecutive days. At the end of the treatment, uteri were harvested for histological and molecular biological analyses. Distribution of proliferative cell nuclear antigen (PCNA) protein in the uterus was observed by immunohistochemistry. Levels of expression of PCNA protein and mRNA in uterine tissue homogenates were determined by Western blotting and real-time polymerase chain reaction, respectively. Our findings indicated that administration of 10 mg/kg/day of quercetin either alone or with estrogen resulted in decreased uterine expression of PCNA protein and mRNA with the percentage of PCNA-positive cells in uterine luminal and glandular epithelia markedly reduced compared with estrogen-only treatment. Changes in uterine morphology were the opposite of changes observed following estrogen treatment. Treatment with 100 mg/kg/day of quercetin either alone or with estrogen resulted in elevated PCNA protein and mRNA expression. In addition, the percentages of PCNA-positive cells in the epithelia, which line the lumen and glands, were increased with morphological features mimicking changes that occur following estrogen treatment. Following 50 mg/kg/day quercetin treatment, the changes observed were in between those changes that occur following 10 and 100 mg/kg/day quercetin treatment. In conclusion, changes in uterine morphology and proliferation following 10 mg/kg/day quercetin treatment could be attributed to quercetin's antiestrogenic properties, while changes that occur following 100 mg/kg/day quercetin treatment could be attributed to

  15. La vagina dentada: una interpretación de la Estela 25 de Izapa y las guacamayas del juego de pelota de Copán The dented vagina: an interpretation for Stela 25 of Izapa and ballcourt macaws of Copan

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    Oswaldo Chinchilla Mazariegos

    2010-01-01

    Full Text Available Las aves representadas en la Estela 25 de Izapa y el juego de pelota de Copán forman el punto de partida para un examen del tema mitológico de la vagina dentada en Mesoamérica, y sus expresiones en el arte y la narrativa. Un amplio conjunto de narraciones mitológicas recopiladas en México y Guatemala sirve como base para un examen de la historia de Siete Guacamayo en el Popol Vuh, la cual se interpreta como una variante de un mito ampliamente difundido, relacionado con el origen del Sol y la Luna. La identificación de asuntos comunes, entre ellos el de la vagina dentada, refuerza la relación entre el mito del Popol Vuh y las representaciones de Izapa y Copán. A la vez, revela detalles poco conocidos sobre el simbolismo sexual presente en los mitos cosmogónicos mesoamericanos.The birds represented at Stela 25 of Izapa and Copan ball court are the starting point for the analysis of the mythological topic focused on the vagina dentata in Ancient Mesoamerica, as well as their expressions on art and narrative. A large body of mythological narratives is used as a basis for the examination of the "Siete Guacamayo" story included in the Popol Vuh, which is interpreted as part of a widely extended myth related with the creation of the Sun and the Moon. The identification of common issues, as the vagina dentata topic, strengthens the links between the Popol Vuh myth and the Izapa and Copan representations. It also reveals less known details on the sexual symbolism included in the Mesoamerican cosmogonic myths.

  16. The Dosimetric Consequences of Intensity Modulated Radiotherapy for Cervix Cancer: The Impact of Organ Motion, Deformation and Tumour Regression

    Science.gov (United States)

    Lim, Karen Siah Huey

    Hypothesis: In intensity modulated radiotherapy (IMRT) for cervix cancer, the dose received by the tumour target and surrounding normal tissues is significantly different to that indicated by a single static plan. Rationale: The optimal use of IMRT in cervix cancer requires a greater attention to clinical target volume (CTV) definition and tumour & normal organ motion to assure maximum tumour control with the fewest side effects. Research Aims: 1) Generate consensus CTV contouring guidelines for cervix cancer; 2) Evaluate intra-pelvic tumour and organ dynamics during radiotherapy; 3) Analyze the dose consequences of intra-pelvic organ dynamics on different radiotherapy strategies. Results: Consensus CTV definitions were generated using experts-in-the-field. Substantial changes in tumour volume and organ motion, resulted in significant reductions in accumulated dose to tumour targets and variability in accumulated dose to surrounding normal tissues. Significance: Formalized CTV definitions for cervix cancer is important in ensuring consistent standards of practice. Complex and unpredictable tumour and organ dynamics mandates daily soft-tissue image guidance if IMRT is used. To maximize the benefits of IMRT for cervix cancer, a strategy of adaptation is necessary.

  17. A vagina-escola: seminário interdisciplinar sobre violência contra a mulher no ensino das profissões de saúde

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    Carmen Simone Grilo Diniz

    2016-03-01

    Full Text Available O ensino de obstetrícia no Brasil tradicionalmente requer que o aluno realize um certo número de procedimentos para que seja avaliado, o que levou a uma cultura de ensino do uso não informado, não consentido, das vaginas das parturientes mais pobres, usuárias do SUS, pelos alunos de medicina e outras profissões, para o treinamento de habilidades cirúrgicas. Tais abusos permanecem normalizados por uma cultura institucional que não os reconhece como violações de direitos, promovendo um ensino de habilidades descolado do ensino de valores e dos direitos das usuárias. Para promover um diálogo entre os setores envolvidos, realizamos em março de 2015 o evento “A Vagina-escola: seminário sobre violência contra a mulher no ensino das profissões de saúde”. O seminário foi especialmente oportuno por acompanhar a recente declaração da Oorganização Mundial de Saúde (OMS “Prevenção e eliminação de abusos, desrespeito e maus-tratos durante o parto em instituições de saúde”, reforçando a urgência do tema na Saúde Coletiva.

  18. Reverse breech extraction versus the standard approach of pushing the impacted fetal head up through the vagina in caesarean section for obstructed labour: A randomised controlled trial.

    Science.gov (United States)

    Nooh, Ahmed Mohamed; Abdeldayem, Hussein Mohammed; Ben-Affan, Othman

    2017-01-31

    The objective of this study was to assess effectiveness and safety of the reverse breech extraction approach in Caesarean section for obstructed labour, and compare it with the standard approach of pushing the fetal head up through the vagina. This randomised controlled trial included 192 women. In 96, the baby was delivered by the 'reverse breech extraction approach', and in the remaining 96, by the 'standard approach'. Extension of uterine incision occurred in 18 participants (18.8%) in the reverse breech extraction approach group, and 46 (47.9%) in the standard approach group (p = .0003). Two women (2.1%) in the reverse breech extraction approach group needed blood transfusion and 11 (11.5%) in the standard approach group (p = .012). Pyrexia developed in 3 participants (3.1%) in the reverse breech extraction approach group, and 19 (19.8%) in the standard approach group (p = .0006). Wound infection occurred in 2 women (2.1%) in the reverse breech extraction approach group, and 12 (12.5%) in the standard approach group (p = .007). Apgar score extraction approach group, and 21 (21.9%) in the standard approach group (p = .015). In conclusion, reverse breech extraction in Caesarean section for obstructed labour is an effective and safe alternative to the standard approach of pushing the fetal head up through the vagina.

  19. Immunohistochemical expression of MIB-1 and PCNA in precancerous and cancerous lesions of uterine cervix

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    Madhu Mati Goel

    2013-01-01

    Full Text Available Background and Objective: The present study was done to analyze the immunoexpression of diagnostic markers (MIB-1: molecular immunology borstel and PCNA: proliferating cell nuclear antigen in grading cervical intraepithelial lesion (CIN and squamous cell carcinoma (SCC in cervix. Setting and Design: Total 150 cervical biopsies were divided into four groups respectively; Group I-Normal (n = 32, Group II- CIN (n = 60, Group III- SCC (n = 44, Group IV- CA cervix (n = 14 respectively. Materials and Methods: These biopsies were stained with monoclonal antibodies by streptavidin--biotin method. Mean labeling index was calculated and grading was performed using the I--III scoring system. Statistical Analysis: Findings were correlated with age and menopausal status. Statistical analysis was done by using student sample′t′ test and analysis of variance (ANOVA by SPSS 10 package. Results: MIB-1 immunostaining was positive in 112/150 (74.6% cases and PCNA in 118 /150 (78.6% cases. Labeling indices showed linear progression from normal to CIN to SCC to cancer lesion. Few cases of low-grade CIN lesion had high proliferative index. A significant positive correlation was found between age and PCNA and MIB-1 values (P < 0.05 when comparison was made for all the cases. Conclusion: These markers may be useful in identifying low-grade CIN lesion with high proliferative index. These cases should be kept for follow up studies so that proper intervention can be taken at an early stage. This method is simple and cost effective and can easily be done in formaline-fixed paraffin embedded tissues in a clinical laboratory for grading CIN and SCC lesions in cervix.

  20. Primitive neuroectodermal tumor of the uterine cervix diagnosed during pregnancy: a rare case with review of literature.

    Science.gov (United States)

    Khosla, Divya; Rai, Bhavana; Patel, Firuza D; Sreedharanunni, Sreejesh; Dey, Pranab; Sharma, Suresh C

    2014-03-01

    Primitive neuroectodermal tumors of the cervix are very rare. A 28-year-old pregnant woman presented with a cervical mass. The tumor was staged as IB2. The biopsy from tumor was suggestive of malignant small round cell tumor. She then underwent termination of pregnancy followed by radical hysterectomy. Based on morphologic and immunohistochemical profile, a diagnosis of peripheral primitive neuroectodermal tumor of the cervix was made. The patient received adjuvant chemotherapy and radiotherapy. The patient is alive and disease-free 33 months post-surgery. The present case highlights the importance of keeping primitive neuroectodermal tumors in the differential diagnosis of small cell neoplasms of the uterine cervix. Pregnancy should not be a barrier to early detection and treatment of this potentially aggressive tumor. The optimal treatment methods have not yet been established because of the rarity of the tumor.

  1. Cervix-to-rectum measuring device in a radiation applicator for use in the treatment of cervical cancer

    Science.gov (United States)

    Fischell, D. R.; Mazique, J. C. (Inventor)

    1981-01-01

    A cervix-to-rectum measuring device to be used in the treatment of cervical cancer is described. It includes a handle and a probe pivotably connected to the handle for insertion in the rectum. The measuring device further includes means for coupling the handle to an intrauterine radiation applicator when the latter is positioned in the uterine cervix and the probe is inserted in the rectum to pivot the handle about the probe. A gear is provided which is adapted to pivot with the probe. A pinion pivotably connected to the handle meshes with the gear. A pointer fixed to the pinion is displaced in response to the pivoting of the handle about the probe, and this displacement can be read from a scale on the handle, providing an indication of the cervix-to-rectum distance.

  2. Progress in Diagnosis and Treatment of Small Cell Carcinoma of the Cervix

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Small cell carcinoma of the cervix (SCCC) belongs to the neuroendocrine carcinomas, and it is a rare gynecological tumor of high-potential malignancy. It has a poorer prognosis compared to cervical squamous cancer or adenocarcinoma, and the therapeutic regimen of the disease differs. Diagnosis is based on pathomorphological characteristics, i.e., the small and round cancer cells (oat cell) which are uniform in shape and size, with the immunohistochemical marker helpful for diagnosis. Combined therapy is first recommended. Postoperative chemotherapy with platinum/etoposide (PE), vincristine/adriamycin/cyclophosphamide (VAC) and taxel/carboplatin (TP) can markedly improve the prognosis of early SCCC patients.

  3. Radiobiological compensation: A case study of uterine cervix cancer with concurrent chemotherapy

    Science.gov (United States)

    Herrera, Higmar; Yañez, Elvia; López, Jesús

    2012-10-01

    The case of a patient diagnosed with uterine cervix cancer is presented as an example of the clinical application of the radiobiological compensation method implemented at Centro Estatal de Cancerología de Durango. Radiotherapy treatment was initially modified to compensate for the chemotherapy component and, as medical complications arose during treatment delivery resulting in an 18 days gap, new compensation followed. All physical and radiobiological assumptions to calculate the Biologically Effective Dose in the external beam and brachytherapy parts of the treatment are presented. Good local control of the tumor was achieved, the theoretical tolerance limits for the organs at risk were not surpassed and the patient manifested no extensive morbidity.

  4. Uncommon sarcomas of the uterine cervix: a review of selected entities

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    Fadare Oluwole

    2006-09-01

    Full Text Available Abstract Sarcomas constitute less than 1% of all cervical malignancies. With over 150 reported cases, rhabdomyosarcomas represent the most commonly reported sarcoma at this location. In this report, a select group of the more uncommon sarcomas of the uterine cervix are reviewed, including all previously reported examples of leiomyosarcoma, liposarcoma, alveolar soft part sarcoma, Ewing sarcoma/primitive neuroectodermal tumor, undifferentiated endocervical sarcoma, and malignant peripheral nerve sheath tumor (MPNST. Emphasis is placed on any distinctive clinicopathologic features of these entities at this unusual location.

  5. Labor-associated gene expression in the human uterine fundus, lower segment, and cervix.

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    Radek Bukowski

    2006-06-01

    Full Text Available BACKGROUND: Preterm labor, failure to progress, and postpartum hemorrhage are the common causes of maternal and neonatal mortality or morbidity. All result from defects in the complex mechanisms controlling labor, which coordinate changes in the uterine fundus, lower segment, and cervix. We aimed to assess labor-associated gene expression profiles in these functionally distinct areas of the human uterus by using microarrays. METHODS AND FINDINGS: Samples of uterine fundus, lower segment, and cervix were obtained from patients at term (mean +/- SD = 39.1 +/- 0.5 wk prior to the onset of labor (n = 6, or in active phase of labor with spontaneous onset (n = 7. Expression of 12,626 genes was evaluated using microarrays (Human Genome U95A; Affymetrix and compared between labor and non-labor samples. Genes with the largest labor-associated change and the lowest variability in expression are likely to be fundamental for parturition, so gene expression was ranked accordingly. From 500 genes with the highest rank we identified genes with similar expression profiles using two independent clustering techniques. Sets of genes with a probability of chance grouping by both techniques less than 0.01 represented 71.2%, 81.8%, and 79.8% of the 500 genes in the fundus, lower segment, and cervix, respectively. We identified 14, 14, and 12 those sets of genes in the fundus, lower segment, and cervix, respectively. This enabled networks of co-regulated and co-expressed genes to be discovered. Many genes within the same cluster shared similar functions or had functions pertinent to the process of labor. CONCLUSIONS: Our results provide support for many of the established processes of parturition and also describe novel-to-labor genes not previously associated with this process. The elucidation of these mechanisms likely to be fundamental for controlling labor is an important prerequisite to the development of effective treatments for major obstetric problems

  6. CLINICAL ASSESSMENT AND CORRILATION OF PAP SMEAR AND LIQUID BASED CYTOLOGY IN BAD CERVIX

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    Khushboo

    2014-10-01

    Full Text Available AIM: Aim of our study to analyze the prevalence of premalignant lesion based on Pap smear and liquid based cytology in patients of bad cervix. OBJECTIVE: To compare sensitivity and specificity of two methods for screening of bad cervix and to know IDR (increase detection rate of cervical cancer by conventional Pap smear and liquid based cytology. MATERIAL AND METHOD: 200 women attending Gynaecology OPD were random selection on the basis of inclusion criteria. All 200 selected women were subjected for down staging through per speculum examination for identification of bad cervix. Pap smears of all 200 selected women were taken and ensured that no local douche, antiseptic cream and no local internal examination was done on the day of test. The prepared smears were then stained according to Papanicolaou's technique. Liquid based cytology smears preparing by using cervical brush 1-1.5cm were inserted into the cervical os until the large outer bristles of the brush touch ectocervix. Data collected for sociodemographic, parity, down staging clinical examination Pap smear and LBC was organised, interpreted and analysis on appropriate statistical software. P value < 0.05 is considered significant RESULT: Analysis revealed that the maximum number of women in our study are from middle age group(31-40yrs, low socioeconomic status, married before 18 yrs, multipara, do not use any contraceptive, uneducated, urban, Muslim population.in this study more abnormal smear is seen in LBC as compared to pap smear. Sensitivity and specificity for LBC is more as compare to pap smear. CONCLUSION: In low resource setting like ours were facilities for radiology, chemo- radiotherapy and supportive care are limited or unavailable. It is important to identify which resources fill healthcare need most effectively and to consider alternative approaches, LBC is strongly advocated in the best interest of public health, by improving the quality of the sample and reducing the

  7. Tuberculosis of the cervix and infertility: report of a rare case.

    Science.gov (United States)

    Guié, P; Iovenitti, P; N'guessan, K; Tegnan, J; Koffi, K; Carta, G; Anongba, S

    2008-01-01

    Tubercolosis is a frequent bacterial infection in less developed countries. Lung and lymph node localisations are common, while the genital apparatus is less involved. In this work a rare case of cervical tuberculosis followed by some lesions causing infertility in a 20-year-old woman is reported. The diagnosis was confirmed by a histological examination from a biopsy of the cervix. The patient was offered six-month antituberculosis therapy which eliminated the cervical lesions. A few years later she came under our care for infertility due to uterine adhesions diagnosed by hysterosalpingography. Now the patient is being treated for infertility complicated by amenorrhoea.

  8. Clinical analysis of alveolar soft-tissue sarcoma of the uterine cervix: a case report

    Institute of Scientific and Technical Information of China (English)

    MU Yu-lan; LIU Ming; SHI Min; ZHAO Xing-bo; YIN Fu-bo; TANG Chun-sheng; Frank D. Yelian

    2010-01-01

    @@ Alveolar soft part sarcoma (ASPS) is a rare tumor that was originally named by Christopherson in 1952.1Until now, fewer than 200 cases have been described in the literature. The ASPS on the uterine cervix is a very rare malignant tumor of the female reproductive organs.Including the first case described by Flint et al2 in 1985,there have been only a few cases reported to date, of which two were in China, and there is a lack of insightful analysis of treatment options. Therefore, to raise awareness of this disease, we performed a detailed literature review, which includes a case encountered by ourselves.

  9. Radiobiological compensation: A case study of uterine cervix cancer with concurrent chemotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Herrera, Higmar; Yanez, Elvia; Lopez, Jesus [Centro Estatal de Cancerologia de Durango, Victoria de Durango, Durango (Mexico); ISSSTE General Hospital Dr. Santiago Ramon y Cajal, Victoria de Durango, Durango (Mexico)

    2012-10-23

    The case of a patient diagnosed with uterine cervix cancer is presented as an example of the clinical application of the radiobiological compensation method implemented at Centro Estatal de Cancerologia de Durango. Radiotherapy treatment was initially modified to compensate for the chemotherapy component and, as medical complications arose during treatment delivery resulting in an 18 days gap, new compensation followed. All physical and radiobiological assumptions to calculate the Biologically Effective Dose in the external beam and brachytherapy parts of the treatment are presented. Good local control of the tumor was achieved, the theoretical tolerance limits for the organs at risk were not surpassed and the patient manifested no extensive morbidity.

  10. MISSING CASES OF CANCER CERVIX IN NEWLY STARTED OUT PATIENT DEPARTMENT OF RADIOTHERAPY, CIMS, BILASPUR (C. G.

    Directory of Open Access Journals (Sweden)

    Hemlata

    2015-10-01

    Full Text Available BACKGROUND: C ancer of the cervix is the second most common cancer among women’s worldwide. In India also, cancer of cervix is second most common cancer among women’s and number one killer. Estimated incidence rate of 22/lac population . [1] C ancer cervix is difficult to cure once symptoms developed. Prognosis is strongly dependent upon the stage of cancer at the time of detection and treatment. In our institute CIMS, Bilaspur (C. G. newly form cancer unit start providing services from August 2013. In our Hospital registry a cross - sectional base line data were collected, it was eye opener that proportion of cancer of cervix registry are less as compared nationwide HBCR & PBCR programme. OBJECTIVE : 1. To find out the reason for low registration of cancer cervix cases in OPD of cancer Unit. 2. To developed / suggest appropriated screening program. MATERIAL AND METHOD S : Retrospective study was carried out to collect all information from - Year 2012 to June 2 015, from medical records & Registers of department of pathology, department of Obstratric & Gynaecology and Medical Record Department, information collected regarding cancer cervix patient in semi structured format and analysed by SPSS software to inquire about cancer of cervix patient. STATISTICAL ANALYSIS : Using SPSS software 11.5 version. Statistic were reported in form of frequency and percentages. RESULT : In this present study out of total diagnosed cervical cancer cases in our institute only 61.11% c ases reach up to radiotherapy department for registration and 38.88% cases are lost during referral and follow - up . Yield of cancer cervix screening camp was only 5.75% and OPD based screening 22.89% with cervical smear cytology, both figure are quit low as cytology is estimated to have a mean sensitivity of 58% and specificity of 69 % [2] both sampling and detection error probably contribute to low to moderate sensitivity of cytology. CONCLUSION: C oordination between

  11. Mid-dose rate intracavitary therapy for uterine cervix cancer with a Selectron; An early experience of Osaka University

    Energy Technology Data Exchange (ETDEWEB)

    Teshima, Teruki; Inoue, Takehiro; Sasaki, Shigeru; Ohtani, Masatoshi; Kozuka, Takahiro; Inoue, Toshihiko; Ikeda, Hiroshi; Yamazaki, Hideya (Osaka Univ. (Japan). Faculty of Medicine); Murayama, Shigeyuki

    1993-05-01

    From May 1991 through September 1992, a total of 17 previously untreated patients with invasive uterine cervix cancer and with intact uterus were treated with mid-dose rate intracavitary therapy administered with a Selectron. Early primary tumor responses for all patients were complete. No acute or subacute radiation injury was observed except one patient with aplastic anemia who developed rectal ulcer. Two patients of Stage IIIb died from tumor because of local, paraaortic lymph node and distant metastases. Our early experience concluded that Selectron MDR can be used for cervix cancer patients as safely and effectively as our previously used high-dose rate machine. (author).

  12. CDC27 protein is involved in radiation response in squamous cell cervix carcinoma.

    Science.gov (United States)

    Rajkumar, T; Gopal, G; Selvaluxmi, G; Rajalekshmy, K R

    2005-10-01

    In the present study, an attempt was made to identify genes involved in radiation response in cervix carcinoma. Differential display technique was used to study the expression profiles of tumour biopsy samples obtained from patients, responding and not responding to treatment. The samples were obtained prior to radiotherapy and subsequent to treatment with Tele-radiation at 10 Gray (Gy). One of the differentially expressed cDNAs, when sequenced was identified to be CDC27. Immuno-histochemical analysis of pre- and post-treated tumour samples from fifteen patients showed the downregulation of expression of CDC27 protein in seven patients. Down-regulation was associated with poorer response to radiotherapy. Cervical cancer cell lines SiHa and C33A were irradiated and their nuclei were stained for expression of CDC27 and analyzed using fluorescent-activated cell sorting (FACS). Down-regulation of CDC27 protein in the irradiated SiHa cell line was associated with greater survival fraction, compared to the irradiated C33A cell line, which had only slight fall in the level of CDC27 protein. This is the first study to suggest a role for CDC27 in radiation response. However, a larger cohort is needed to further confirm the value of CDC27 protein as a predictive marker, for radiation response in cervix cancer.

  13. [Preoperative concurrent chemotherapy and radiation therapy in cervix cancer: preliminary results].

    Science.gov (United States)

    Kochbati, Lotfi; Ben Ammar, Chiraz Nasr; Benna, Farouk; Hechiche, Monia; Boussen, Hamouda; Besbes, Mounir; Ben Abdallah, Mansour; Rahal, Khaled; Ben Ayed, Farhat; Ben Romdhane, Khaked; Maalej, Mongi

    2005-03-01

    This is a retrospective study of patients treated for cervix cancer staged IB2, IIA or IIB with bulky tumor (> 4cm). Treatment was concurrent radiotherapy (45Gy with 1,8Gy daily fraction) and chemotherapy (5 cycles of Platinum 40mg/m2/week). All patients underwent Brachytherapy (15Gy on the reference isodose according to Paris system) followed by surgery (radical abdominal hysterectomy and bilateral pelvic lymphadenectomy: Piver 3) Between October 1999 and December 2002, forty five patients were treated in this protocol. Median age was 46 years (21- 68). Histology was squamous cell carcinoma in 93% and glandular carcinoma in 7%. Average external radiation dose was 44Gy (20-50). Ninety three percent of patients had at least 3 cycles of chemotherapy and 46,5% received the planned 5 cycles. On the operative specimens, there was 62,5% complete response and only 7 pelvic node involvement (17,5%). Four postoperative complications were noted (one vascular injury, one urinary fistula, one phlebitis and one lymph collection). Preoperative combined radiotherapy and chemotherapy in the early bulky stages of uterine cervix cancer is well tolerated and "gives" a high rate of sterilisation. There was no increase in surgical morbidity.

  14. Role of surgery in breast metastasis from carcinoma of the cervix

    Directory of Open Access Journals (Sweden)

    Parveen Yadav

    2011-01-01

    Full Text Available Carcinoma of the cervix is the most common malignancy among women in India. Although metastatic disease is common, metastasis to breast is rare. A limited number of case reports are published in the world literature. Most of the previous reports of metastatic cervical carcinoma to breast are either autopsy series or widely disseminated disease where no treatment options were available. A rare case of cervical carcinoma presenting as metastasis in breast is reported here where palliative mastectomy improved the general condition of the patient. A female patient aged 58 years was diagnosed and treated for cervical carcinoma, FIGO stage 2B. Four months after the treatment which included both external beam and intracavitory radiotherapy, the patient presented with breast and lung metastasis. Palliative mastectomy was done which improved the general condition of the patient. Metastatic carcinoma of the cervix can present as a case of breast carcinoma. In an appropriate setting, this possibility should be kept in mind. Palliative mastectomy should be offered for patients of cervical carcinoma with metastasis to breast when needed.

  15. Uterine papillary serous carcinoma following radiation therapy for carcinoma of cervix: a case report.

    Science.gov (United States)

    Park, M. H.; Cho, S. H.; Kang, H. J.; Kim, S. R.; Hwang, Y. Y.

    2000-05-01

    Uterine papillary serous carcinoma (UPSC) is a clinically aggressive and morphologically distinctive variant of endometrial carcinoma that has been recognized recently as a distinct entity. The association between radiation therapy (RT) and UPSC is rarely described in the literature. We describe the clinicopathologic features of a 71-year-old patient with UPSC that developed 15 years after radiation therapy for squamous cell carcinoma of cervix, stage IIB. In the subtotal hysterectomy specimen the endometrium was irregular with multifocally raised masses. Microscopically, the tumor was composed of high-grade papillary serous carcinoma focally admixed with solid transitional cell carcinomatous areas and multifocal intraepithelial carcinoma in adjacent atrophic endometrium. The tumor exhibited diffuse infiltrative growth with frequent lymphatic tumor emboli in the myometrium. Immunohistochemical staining for p53 and c-erbB-2 were positive in about 70% of the tumor cells. Carcinoembryonic antigen (CEA) was focally positive. Ki-67 positive cells were present in about 60% of the tumor cells. The tumor directly extended to the cervix and perirectal soft tissue and metastasized to the omentum. Intraoperative pelvic washing cytology was positive for papillary adenocarinoma cells. The possible etiologic role of radiation is discussed, and the literature on endometrial carcinomas developing after RT is reviewed.

  16. Diagnosis of uterine cervix cancer using Müller polarimetry: a comparison with histopathology

    Science.gov (United States)

    Rehbinder, Jean; Deby, Stanislas; Haddad, Huda; Teig, Benjamin; Nazac, André; Pierangelo, Angelo; Moreau, François

    2015-07-01

    Today around 275000 women a year in the world keep dying from the cancer of uterine cervix due to the difficulty to meet the logistic requirements of an organized screening in the developing world. Polarimetric imaging is a new promising technique with a tremendous potential for applications in biomedical diagnostics: it is sensitive to slight morphological changes in tissues, can provide wide field images for the screening and requires light sources such as a LED for example. This work intends to characterize the polarimetric response of the uterine cervix in its healthy and pathological states. An extensive series of ex-vivo measurements is in progress the Kremlin Bicêtre hospital near Paris using an imaging multispectral Mueller polarimeter in backscattering configuration. The goal of this study is to evaluate the performances of polarimetric imaging technique in terms of sensitivity and specificity for the detection of healthy epithelia (Healthy Squamous epithelium and Malpighian Metaplasia) with respect to the diagnosis provided by pathologists from histology slides as the "gold standard". We show that, at λ=550nm, performances as high as 62% sensitivity and 64% specificity are achieved by optimizing a simple threshold on the scalar retardance values.

  17. Surgical treatments for post-irradiation intestinal injury in uterine cervix cancer patients

    Energy Technology Data Exchange (ETDEWEB)

    Nozaki, Isao; Yokoyama, Nobuji; Takashima, Shigemitsu [National Shikoku Cancer Center Hospital, Matsuyama, Ehime (Japan)

    1997-06-01

    We examined 19 patients with post-irradiation intestinal injury in the uterine cervix cancer for 12 years between 1985 and 1996. We discuss the usefulness and complications of surgery, mainly colostomy. The patients aged from 36 to 80 (average age 61) were treated, and their disease states were 12 cases of rectovaginal fistula, 2 of small intestinal fisfula, 1 of rectum posterior membranous fistula, 3 of proctostenosis, and 14 of proctitis with hemorrhage (including duplication). Surgical methods used were 18 cases of colostomy (2 cases were treated under peritoneum mirror) and 2 of enterocolostomy (including duplication). Eleven out of 19 patients who underwent surgery are alive now. Generally the post-irradiation intestinal injury was intractable, and the method of treatments were limited due to the coexistence of various diseases. The colostomy is safe and less invasive. Therefore patients with uterine cervix cancer having various complications can obtain high quality of life (QOL) such as the improvement of anemia and/or the increase of digestion by the colostomy. (K.H.)

  18. MR imaging features and staging of neuroendocrine carcinomas of the uterine cervix with pathological correlations

    Energy Technology Data Exchange (ETDEWEB)

    Duan, Xiaohui; Zhang, Xiang; Hu, Huijun; Li, Guozhao; Wang, Dongye; Zhang, Fang; Shen, Jun [Sun Yat-Sen University, Department of Radiology, Sun Yat-Sen Memorial Hospital, Guangzhou (China); Ban, Xiaohua [Sun Yat-Sen University, Medical Imaging and Minimally Invasive Interventional Center and State Key Laboratory of Oncology in Southern China, Cancer Center, Guangzhou, Guangdong (China); Wang, Charles Qian [Sun Yat-Sen University, Department of Radiology, Sun Yat-Sen Memorial Hospital, Guangzhou (China); University of New South Wales, JMO, Westmead Hospital, Sydney (Australia)

    2016-12-15

    To determine MR imaging features and staging accuracy of neuroendocrine carcinomas (NECs) of the uterine cervix with pathological correlations. Twenty-six patients with histologically proven NECs, 60 patients with squamous cell carcinomas (SCCs), and 30 patients with adenocarcinomas of the uterine cervix were included. The clinical data, pathological findings, and MRI findings were reviewed retrospectively. MRI features of cervical NECs, SCCs, and adenocarcinomas were compared, and MRI staging of cervical NECs was compared with the pathological staging. Cervical NECs showed a higher tendency toward a homogeneous signal intensity on T2-weighted imaging and a homogeneous enhancement pattern, as well as a lower ADC value of tumour and a higher incidence of lymphadenopathy, compared with SCCs and adenocarcinomas (P < 0.05). An ADC value cutoff of 0.90 x 10{sup -3} mm{sup 2}/s was robust for differentiation between cervical NECs and other cervical cancers, with a sensitivity of 63.3 % and a specificity of 95 %. In 21 patients who underwent radical hysterectomy and lymphadenectomy, the overall accuracy of tumour staging by MR imaging was 85.7 % with reference to pathology staging. Homogeneous lesion texture and low ADC value are likely suggestive features of cervical NECs and MR imaging is reliable for the staging of cervical NECs. (orig.)

  19. In vitro multifrequency electrical impedance measurements and modelling of the cervix in late pregnancy.

    Science.gov (United States)

    Avis, N J; Lindow, S W; Kleinermann, F

    1996-11-01

    Idiopathic preterm labour is the greatest single perinatal problem occurring in an unpredictable 6-8% of all pregnancies and accounting for 75% of all perinatal deaths. Preterm cervical softening is used clinically as an important indicator of cervical dysfunction but the subjective nature of present clinical assessment methods prevents reliable prediction of preterm labour. This paper reports the finding of a pilot investigation concerned with obtaining quantitative measurements of the in vitro electrical impedance of the cervix using a four-electrode multifrequency impedance measurement system. Impedance measurement obtained from six samples of cervical tissue taken from different subjects of caesarean section were fitted to the Cole equation and parameters derived to describe the ratio of extra- versus intracellular impedance and the characteristic frequency. Subjects at term display a lower extra- versus intracellular impedance ratio than the preterm subjects. This appears consistent with the expected increase in the hydration of the cervix approaching term and the resulting decrease in the extracellular impedance. Further studies are required to determine if multifrequency electrical impedance tomography could be used as a non-invasive screening test for preterm labour.

  20. Preparation for induction of labour of the unfavourable cervix with Foley catheter compared with vaginal prostaglandin.

    Science.gov (United States)

    Thomas, I L; Chenoweth, J N; Tronc, G N; Johnson, I R

    1986-02-01

    Ripening of the unfavourable cervix prior to induction of labour using traction on a Foley catheter (32 patients) was compared with 40 mg of prostaglandin F2 alpha in Tylose gel applied to the external cervical os and held in place for 12 hours with a vaginal diaphragm (25 patients). Each patient in the above groups had a modified Bishop score of 0-3 and was randomly allocated to one or other group. Comparison was made with a further 25 patients in whom the cervical score was 4-6. Timing of amniotomy and commencement of Syntocinon infusion were equivalent for all patients. Prostaglandins conferred no advantage over Foley catheter in terms of amniotomy-delivery interval, operative delivery rate, and condition of the baby one minute after birth. The disadvantages of prostaglandins for cervical ripening are a longer preparation-delivery interval, and cost ($77 versus $4.75 for the Foley catheter). Currently, prostaglandins are not officially approved for use in Australia for induction of labour. It is suggested, therefore, that the Foley catheter is preferable for ripening the unfavourable cervix as a prelude to amniotomy.

  1. Abnormal excessive per vagina (PV) bleeding on Esmya-selective progesterone receptor modulator (SPRM) in a symptomatic patient with uterine fibroid.

    Science.gov (United States)

    Matytsina-Quinlan, Lyubov; Matytsina, Laura

    2015-05-14

    A woman in her late 40s presented with excessive per vagina (PV) bleeding and uterine fibroid. She reported excessive PV bleeding after starting Esmya; she was brought in by ambulance to the emergency department with profuse bleeding. Abnormal uterine bleeding (AUB) developed after selective progesterone receptor modulator (SPRM) administration in this symptomatic patient with uterine fibroid. The drug was withheld and surgical treatment considered. Progressive deterioration of PV bleeding after receiving SPRM led to an urgent laparoscopic total hysterectomy, which had to be postponed due to severe anaemia. Surgery took place regardless because the excessive bleeding continued. Histology revealed a 6 cm submucosal uterine fibroid (SMUF) and adenomyosis. Physicians prescribing SPRMs to stop PV bleeding should be aware of potential AUB, which could lead to urgent hysterectomy. The mechanism of action of SPRMs is not clearly understood. Awareness of the side effects of Esmya, such as AUB, must be kept in mind when administering SPRMs.

  2. Candida colonization of the vagina of HIV-seropositive pregnant women and their seronegative counterparts at selected health-care centers in Akure, Ondo State, Nigeria

    Directory of Open Access Journals (Sweden)

    Blessing Itohan Ebhodaghe

    2016-01-01

    Full Text Available Background: Candida colonization of the vagina is a risk factor in pregnancy. Candida isolates have been implicated in adverse pregnancy outcomes. The study determined the incidence of Candida species recovered from the vagina of HIV-seropositive and HIV-seronegative pregnant women that attended antenatal clinics in Akure, Ondo State between November 2014 and December 2015. Materials and Methods: Two hundred and forty pregnant women aged 19–43 participated in the study, which included 114 HIV-seropositive subjects with mean age 31.81 years and 126 HIV-seronegative subjects with mean age 29.05 years as controls. High vaginal swab was collected from each subject using sterile cotton-tipped applicator, streaked onto Mycological Agar - supplemented with streptomycin. Each sample was incubated 24 h for yeast and 72–120 h for the growth of molds. Yeast colonies that grew on Mycological Agar were picked and studied. Thereafter, colonies resembling Candida were identified using sugar assimilation and fermentation. Candida isolates were further speciated using Candida Ident Agar, modified. Antifungal resistance profile was identified with azoles, polyenes, echinocandins, flucytosine, and griseofulvin drugs. Antifungal resistant assay was determined by disc and agar well diffusion. Results: Altogether, 157 Candida isolates were recovered from HIV-seropositive and HIV-seronegative subjects. Candida albicans constituted 46.5%, Candida dubliniensis and Candida glabrata 15.3% each, Candida krusei 12.1%, Candida spp. 5.7%, and Candida tropicalis and Candida pseudotropicalis 2.5% each. Antifungal resistance was widespread with azoles, polyenes, echinocandins, flucytosine, and griseofulvin. Conclusion: C. albicans was the predominant isolate recovered (17.2% HIV-seropositive and 29.3% HIV-seronegative subjects. Widespread antifungal resistance seems high and suggests possible abuse of these drugs.

  3. PATIENTS WITH SQUAMOUS-CELL VERSUS ADENO(SQUAMOUS) CARCINOMA OF THE CERVIX, WHAT FACTORS DETERMINE THE PROGNOSIS

    NARCIS (Netherlands)

    TINGA, DJ; BOUMA, J; AALDERS, JG

    1992-01-01

    Patients with squamous cell carcinoma of the cervix FIGO stages IB to IV (n = 306) were compared to patients with adeno(squamous) carcinoma (n = 70). There was no difference between the mean ages of the groups. In the patients who underwent radical surgical treatment, whether or not in combination w

  4. MRI-assisted cervix cancer brachytherapy pre-planning, based on application in paracervical anaesthesia: final report

    Directory of Open Access Journals (Sweden)

    Petric Primoz

    2014-09-01

    Full Text Available Background. Optimal applicator insertion is a precondition for the success of cervix cancer brachytherapy (BT. We aimed to assess feasibility and efficacy of MRI-assisted pre-planning, based on applicator insertion in para-cervical anaesthesia (PCA.

  5. Role of vaginal progesterone in reducing the rate of preterm labour in women with a sonographic short cervix

    Directory of Open Access Journals (Sweden)

    Kirtirekha Mohapatra

    2015-12-01

    Conclusions: Vaginal progesterone started from midtrimester in pregnant ladies with short cervix with previous history of midtrimester abortion or preterm labour is effective in reducing the rate of preterm birth. [Int J Reprod Contracept Obstet Gynecol 2015; 4(6.000: 1793-1797

  6. Characterization of human papillomavirus infection, P53 and Ki-67 expression in cervix cancer of Mozambican women.

    Science.gov (United States)

    Carrilho, Carla; Gouveia, Patricia; Cantel, Martha; Alberto, Matos; Buane, Landim; David, Leonor

    2003-01-01

    In this study, we aimed at evaluating the distribution of HPV types and the expression of P53 and Ki-67 in cervix carcinomas of Mozambican women. Fourty-seven invasive carcinomas, 10 CIN III, and 10 normal cervix were studied. P53 and Ki-67 expression was examined immunohistochemically. HPV infection and HPV types were detected by PCR (GP5+/bio-GP6+) and enzyme-immunoassay, respectively. Expression of P53 and Ki-67 and detection of HPV were as follows: normal cervix--0%, 10%, and 0%, respectively; CIN III--10%, 0%, and 100%, respectively; invasive carcinomas--50%, 55.5%, and 70%, respectively. HPV 16 was identified in 54% of invasive carcinomas, HPV 31, 33, 35, and 45 in 23%, "unidentified" HPV in 19%, and HPV 18 in 4% of invasive carcinomas. No significant associations were observed between P53 expression, Ki-67 expression, and HPV infection. In conclusion, we observed a high frequency of HPV infection in CIN III lesions and invasive carcinomas from Mozambican women, with HPV 16 representing the most frequent viral type. HPV status was not related to P53 and Ki-67 expression. Both P53 and Ki-67 are associated with invasive cervix carcinomas, mainly of the squamous keratinizing histotype.

  7. Risk factors for cancer cervix among rural women of a hilly state: A case-control study

    Directory of Open Access Journals (Sweden)

    Anita Thakur

    2015-01-01

    Full Text Available In Himachal Pradesh, cancer cervix is a major public health problem since it ranks as the number one female cancer. A case-control study of 226 newly diagnosed, histopathologically confirmed cases of cancer cervix and equal number of matched controls was conducted at Regional Cancer Center, Himachal Pradesh during the period from July 2008 to October 2009 with the objective to study the common factors associated with cancer cervix. Univariate analysis identified 10 risk factors associated significantly with the disease. On multiple logistic regression, however, only seven risk factors were found to be associated significantly with the disease. These were: Age at birth of first child, spacing between two children, age at marriage, literacy, socioeconomic status, multiparity, and poor genital hygiene. Risk factors such as poor genital hygiene, age at birth of first child <19 years, early marriage, illiteracy, multiparity, and low socioeconomic status were highly prevalent in the study subjects and were found to be significantly associated with cancer cervix.

  8. Immunohistochemical distribution of oestrogen and progesterone receptors and tissue concentrations of oestrogens in the cervix of non-pregnant cows

    NARCIS (Netherlands)

    Breeveld-Dwarkasing, V.N.A.; Boer-Brouwer, de M.; Mostl, E.; Soede, N.M.; Weijden, van der G.C.; Taverne, M.A.M.; Dissel-Emiliani, van F.M.F.

    2002-01-01

    An immunohistochemical study of the expression of oestrogen (ER) and progesterone receptors (PR) in different regions along the longitudinal and vertical axes of the cervix of non-pregnant cows was performed. Animals were separated into two groups depending on the presence or absence of a functional

  9. The value of loop electrosurgical conization in the treatment of stage IA1 microinvasive carcinoma of the uterine cervix.

    NARCIS (Netherlands)

    Bekkers, R.L.M.; Keijser, K.G.G.; Bulten, J.; Hanselaar, A.G.J.M.; Schijf, C.P.T.; Boonstra, H.; Massuger, L.F.A.G.

    2002-01-01

    The objective of this study is to assess the value of Loop Electrosurgical Conization (LEC) in the treatment of stage IA1 microinvasive squamous cell carcinoma (MIC) of the uterine cervix. Retrospectively, 82 patients with FIGO stage IA1 MIC, primarily treated with LEC on see and treat basis, were a

  10. Robotically assisted total laparoscopic radical trachelectomy for fertility sparing in stage IB1 adenosarcoma of the cervix.

    Science.gov (United States)

    Geisler, John P; Orr, Curtis J; Manahan, Kelly J

    2008-10-01

    Adenosarcomas are rare cervical tumors with unknown optimal treatment, which often affects young women. A 23-year-old woman was found to have a stage IB1 adenosarcoma of the cervix. She underwent a robotically assisted total laparoscopic radical trachelectomy with the placement of abdominal cerclage for the sparing of fertility.

  11. Investigating the mechanical function of the cervix during pregnancy using finite element models derived from high-resolution 3D MRI.

    Science.gov (United States)

    Fernandez, M; House, M; Jambawalikar, S; Zork, N; Vink, J; Wapner, R; Myers, K

    2016-01-01

    Preterm birth is a strong contributor to perinatal mortality, and preterm infants that survive are at risk for long-term morbidities. During most of pregnancy, appropriate mechanical function of the cervix is required to maintain the developing fetus in utero. Premature cervical softening and subsequent cervical shortening are hypothesized to cause preterm birth. Presently, there is a lack of understanding of the structural and material factors that influence the mechanical function of the cervix during pregnancy. In this study we build finite element models of the pregnant uterus, cervix, and fetal membrane based on magnetic resonance imagining data in order to examine the mechanical function of the cervix under the physiologic loading conditions of pregnancy. We calculate the mechanical loading state of the cervix for two pregnant patients: 22 weeks gestational age with a normal cervical length and 28 weeks with a short cervix. We investigate the influence of (1) anatomical geometry, (2) cervical material properties, and (3) fetal membrane material properties, including its adhesion properties, on the mechanical loading state of the cervix under physiologically relevant intrauterine pressures. Our study demonstrates that membrane-uterus interaction, cervical material modeling, and membrane mechanical properties are factors that must be deliberately and carefully handled in order to construct a high quality mechanical simulation of pregnancy.

  12. CD4 T cell depletion at the cervix during HIV infection is associated with accumulation of terminally differentiated T cells.

    Science.gov (United States)

    Gumbi, P P; Jaumdally, S Z; Salkinder, A L; Burgers, W A; Mkhize, N N; Hanekom, W; Coetzee, D; Williamson, A; Passmore, J S

    2011-12-01

    In blood, the accumulation of terminally differentiated (TD) T cells during HIV infection is associated with CD4 T cell loss and HIV disease progression. Here, we investigated the maintenance and functional characteristics of memory T cells at the cervix. We found that CD4 T cell depletion at the cervix mirrors CD4 depletion in blood. In all women, depletion of CD4 T cells at the cervix was associated with significant reductions in CD45RA- CCR7+ (central memory [CM]) T cells and the accumulation of CD45RA+ CCR7- (TD T cells). We determined whether inflammation in the genital tract was associated with the local differentiation of T cells at the cervix. In uninfected women, genital tract inflammation was associated with the accumulation of CD45RA- CCR7+ CM CD4 T cells and reduced frequencies of CD45RA+ CCR7- TD cells at the cervix. This finding may reflect the fact that, in the absence of HIV infection, TD T cells may be slowly lost in the presence of genital inflammation, while CD45RA- CCR7+ CM T cells are recruited to replenish the diminishing CD4 T cell pool. Following global stimulation with phorbol myristate acetate (PMA)-ionomycin, we noted a significant interleukin 2 (IL-2) deficit in both cervical and blood CD4 T cells from HIV-infected women compared to uninfected women, while gamma interferon (IFN-γ) production was similar, irrespective of HIV status. Few HIV-infected women had detectable IFN-γ and IL-2 HIV-specific T cell responses at the cervix, and these responses were significantly lower in magnitude than the corresponding responses in blood. These data suggest that CD4 depletion was associated with the accumulation of terminally differentiated T cell phenotypes at the cervical mucosa defective in their ability to produce IL-2. CD4 depletion and compromised immunity at the cervix may be accompanied by progressive decline of central memory-like T cells and development of T cells toward terminally differentiated phenotypes.

  13. Enzymatic collagen degradation in the pregnant guinea pig cervix during physiological maturation of the cervix and after local application of prostaglandins.

    Science.gov (United States)

    Rath, W; Adelmann-Grill, B C; Osmers, R; Kuhn, W

    1989-09-01

    The role of enzymatic collagen degradation in prostaglandin-induced and physiological cervical ripening was studied in guinea pigs. The cervices were removed from (a) 8 non-pregnant guinea pigs, (b) 8 animals at day 45 of pregnancy, (c) 14 pregnant animals of comparable gestational age which had either an intracervical application of 0.2 ml 5% tylose or 10 micrograms sulprostone gel, and (d) 8 guinea pigs at day 63 to 65 of pregnancy. Collagenase activity was assayed in a highly specific and sensitive system using native collagen type I as substrate. Protease activity was measured by the method of Green and Shaw. Collagen fragments were identified by SDS-polyacrylamide electrophoresis (SDS-PAGE) of acetic-soluble fractions. Collagenase and protease activities were found in all extracts from the different groups. However, there were no differences in enzymatic activities between the non-pregnant, early-pregnant and late-pregnant cervical specimens. Prostaglandin pre-treatment of the cervix led to no significant increase in either collagenase or protease activity as compared to the control groups. The absence of typical collagen degradation products in the SDS-PAGE suggested that no significant collagen breakdown had taken place. In contrast to previously published literature, we conclude that enzymatic collagen degradation is unlikely to be a key factor in prostaglandin-induced and physiological cervical ripening.

  14. The role of neoadjuvant chemotherapy in the management of locally advanced cervix cancer: a systematic review

    Directory of Open Access Journals (Sweden)

    Mohammed Osman

    2014-09-01

    Full Text Available Cervical cancer is the second most common cancer in women. Neoadjuvant chemotherapy for patients with locally advanced cervix cancer has comparable benefits to concurrent chemoradiotherapy (CCRT, but with fewer side effects. This systematic review aims to provide a comprehensive summary of the benefits of neoadjuvant chemotherapy for the management of locally advanced cervix cancer from stage IB2 (tumor >4.0 cm to IIIB (tumor extending to the pelvic wall and/or hydronephrosis. Our primary objective was to assess benefits in terms of survival. The data source included the USA national library of medicine, Medline search, and the National Cancer Institute PDQ Clinical Protocols. Inclusion criteria for consideration in the current systematic review included studies published between January 1997 and December 2012. In terms of histology, they had to be focused on squamous cell carcinoma, adenosquamous carcinoma, and/or adenocarcinoma. Patients should be either chemotherapy naïve or cervix cancer chemotherapy naïve, and have a performance status ≤2. The search in the above-mentioned scientific websites led to identify 49 publications, 19 of which were excluded, as they did not meet the inclusion criteria of this systematic review. Therefore only 30 studies were deemed eligible. Data was collected from 1760 patients enrolled in the current systematic review study. The mean age was 45.2 years. The mean tumor size was 4.7 cm. The most commonly used chemotherapies were cisplatin doublets. Paclitaxel was the most commonly used chemotherapeutic agent in the doublets. The mean chemotherapy cycles were 2.7. After chemotherapy, patients underwent surgery after a mean time of 2.5 weeks. The standard operation was radical hysterectomy with pelvic lymphadenectomy. Chemotherapy achieved an objective response rate of 84%. The 5-year progression-free survival and overall survival were 61.9% and 72.8% respectively. The treatment protocol was associated

  15. Comparison of the Quality of Pap smear Slides Taken from the Cervix Using Two Fixation Methods

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    Keshavarz Najmeh

    2016-12-01

    Full Text Available Introduction: Pap smear or Pap test is a cytological method based on sampling from the cervix (or neck of the womb in women and preparation of smear and direct observation under microscope. Pap smear is now used as a common test for screening in women. Since the Pap smear method has been used as a screening test for early detection of cervical cancer, the incidence of aggressive cervical cancer and mortality caused by it has reduced by 70%. So, conducting of experiments to improve the quality of prepared slides and reduction of red blood cells by proper fixators for better diagnosis of the disease are the purposes of this research. This study is aimed to comprise the adequacy of Pap smear taken from the cervix using conventional fixative spray and Carnoy’s fixative methods. Methods: this study was performed on 202 blood Pap smear slides taken from 101 cervixes of women with abnormal uterine bleeding. These patients referred to Zeinabieh and Shahid Faghihi Hospitals related to Shiraz University of Medical Sciences in 2012 and 2013. After observation of two bleedings, two Pap smear slides were prepared from each sample; both of prepared slides from cervical cells of each sample were fixed at first by conventional fixative spray and one of them was then placed in Carnoy’s solution for 22 minutes and air-dried. The slides were then stained with Papanicolaou stain and examined by two different cytologists in a double-blind fashion. The obtained data were analyzed by SPSS. Results: the results showed that the presence of squamous cells (p=0.004, glandular cells (p=0.001, transformation zone cells (p=0.001, cellular inadequacy (p=0.002, distribution of inflammatory cells (p=0.001 and metaplastic cells (p=0.001 were more on the spray-fixed slides. The mean percentage of blood on the slides (p=0.091 and distribution of microbial agents (p=1 on the spray-fixed and Carnoy’s-fixed slides did not show statistically a significant difference. Discussion

  16. CD4 T Cell Depletion at the Cervix during HIV Infection Is Associated with Accumulation of Terminally Differentiated T Cells ▿

    OpenAIRE

    Gumbi, P. P.; Jaumdally, S. Z.; Salkinder, A. L.; Burgers, W. A.; Mkhize, N. N.; Hanekom, W; Coetzee, D; Williamson, A; Passmore, J. S.

    2011-01-01

    In blood, the accumulation of terminally differentiated (TD) T cells during HIV infection is associated with CD4 T cell loss and HIV disease progression. Here, we investigated the maintenance and functional characteristics of memory T cells at the cervix. We found that CD4 T cell depletion at the cervix mirrors CD4 depletion in blood. In all women, depletion of CD4 T cells at the cervix was associated with significant reductions in CD45RA− CCR7+ (central memory [CM]) T cells and the accumulat...

  17. Anatomy and histology of apical support: a literature review concerning cardinal and uterosacral ligaments.

    Science.gov (United States)

    Ramanah, Rajeev; Berger, Mitchell B; Parratte, Bernard M; DeLancey, John O L

    2012-11-01

    The objective of this work was to collect and summarize relevant literature on the anatomy, histology, and imaging of apical support of the upper vagina and the uterus provided by the cardinal (CL) and uterosacral (USL) ligaments. A literature search in English, French, and German languages was carried out with the keywords apical support, cardinal ligament, transverse cervical ligament, Mackenrodt ligament, parametrium, paracervix, retinaculum uteri, web, uterosacral ligament, and sacrouterine ligament in the PubMed database. Other relevant journal and textbook articles were sought by retrieving references cited in previous PubMed articles. Fifty references were examined in peer-reviewed journals and textbooks. The USL extends from the S2 to the S4 vertebra region to the dorsal margin of the uterine cervix and/or to the upper third of the posterior vaginal wall. It has a superficial and deep component. Autonomous nerve fibers are a major constituent of the deep USL. CL is defined as a perivascular sheath with a proximal insertion around the origin of the internal iliac artery and a distal insertion on the cervix and/or vagina. It is divided into a cranial (vascular) and a caudal (neural) portions. Histologically, it contains mainly vessels, with no distinct band of connective tissue. Both the deep USL and the caudal CL are closely related to the inferior hypogastric plexus. USL and CL are visceral ligaments, with mesentery-like structures containing vessels, nerves, connective tissue, and adipose tissue.

  18. Human papillomavirus and gastrointestinal cancer: A review

    Science.gov (United States)

    Bucchi, Dania; Stracci, Fabrizio; Buonora, Nicola; Masanotti, Giuseppe

    2016-01-01

    Human papillomavirus (HPV) is one of the most common sexually transmitted infections worldwide. Exposure to HPV is very common, and an estimated 65%-100% of sexually active adults are exposed to HPV in their lifetime. The majority of HPV infections are asymptomatic, but there is a 10% chance that individuals will develop a persistent infection and have an increased risk of developing a carcinoma. The International Agency for Research on Cancer has found that the following cancer sites have a strong causal relationship with HPV: cervix uteri, penis, vulva, vagina, anus and oropharynx, including the base of the tongue and the tonsils. However, studies of the aetiological role of HPV in colorectal and esophageal malignancies have conflicting results. The aim of this review was to organize recent evidence and issues about the association between HPV infection and gastrointestinal tumours with a focus on esophageal, colorectal and anal cancers. The ultimate goal was to highlight possible implications for prognosis and prevention. PMID:27672265

  19. The role of human papilloma virus in urological malignancies.

    Science.gov (United States)

    Heidegger, Isabel; Borena, Wegene; Pichler, Renate

    2015-05-01

    Human papillomavirus (HPV) is associated with cancer of the cervix uteri, penis, vulva, vagina, anus and oropharynx. However, the role of HPV infection in urological tumors is not yet clarified. HPV appears not to play a major causative role in renal and testicular carcinogenesis. However, HPV infection should be kept in mind regarding cases of prostate cancer, as well as in a sub-group of patients with bladder cancer with squamous differentiation. Concerning the role of HPV in penile cancer incidence, it is a recognized risk factor proven in a large number of studies. This short review provides an update regarding recent literature on HPV in urological malignancies, thereby, also discussing possible limitations on HPV detection in urological cancer.

  20. Thyroid Gland Metastasis from Cancer of the Uterine Cervix: An Extremely Rare Case Report.

    Science.gov (United States)

    Celik, Suleyman Utku; Besli, Dilara; Sak, Serpil Dizbay; Genc, Volkan

    2016-01-01

    The thyroid gland is a relatively uncommon site for a metastatic disease, although it is richly supplied with blood. The metastases may originate from various primary sites, mainly kidney, lung, head and neck, and breast. Thyroid metastasis from cervical carcinomas is extremely rare; and only a few cases have been previously reported in the literature. In patient with thyroid nodules and an oncological history, the possibility of thyroid metastasis should be seriously considered. Despite the rarity of the metastasis of cervical carcinoma to the thyroid, it is difficult to say appropriate treatment approach for these lesions. When managing such patients, decision-making should balance the possibility of gaining long-term survival against estimation of the aggressiveness of the disease and its possible complications. Here, a case of thyroid metastasis from a squamous cell carcinoma of the uterine cervix presenting with cervical mass and difficulty in swallowing and its treatment is reported.

  1. Seizure following chemotherapy (paclitaxel and cisplatin in a patient of carcinoma cervix

    Directory of Open Access Journals (Sweden)

    Rohitashwa Dana

    2016-01-01

    Full Text Available Cisplatin and paclitaxel both can cause peripheral neurotoxicity as an adverse effect; however, central nervous system neurotoxicity in the form of seizures is rare. We report a case of a 36-year-old female patient of metastatic carcinoma cervix, who developed seizure shortly after cisplatin infusion. Her laboratory investigations were within normal limits. Computed tomography scan and magnetic resonance imaging of the brain did not reveal brain primary metastasis or meningeal carcinomatosis. She had no complaints of fever, no signs and symptoms of infection, and no history of seizure nor was she on any medication predisposing to such an event. Excluding several causes, seizure was thought to be most likely related to the chemotherapy and cisplatin was the more likely agent in view of observed temporal relationship with the adverse event.

  2. Can reduction of uncertainties in cervix cancer brachytherapy potentially improve clinical outcome?

    DEFF Research Database (Denmark)

    Nesvacil, Nicole; Tanderup, Kari; Lindegaard, Jacob C

    2016-01-01

    AIM: The aim of this study was to quantify the impact of different types and magnitudes of dosimetric uncertainties in cervix cancer brachytherapy (BT) on tumour control probability (TCP) and normal tissue complication probability (NTCP) curves. MATERIALS AND METHODS: A dose-response simulation....../NTCP model. Systematic uncertainties of 3-20% and random uncertainties with a 5-30% standard deviation per BT fraction were analysed. RESULTS: Systematic dose uncertainties of 5% lead to a 1% decrease/increase of TCP/NTCP, while random uncertainties of 10% had negligible impact on the dose-response curve...... at clinically relevant dose levels for target and OAR. Random OAR dose uncertainties of 30% resulted in an NTCP increase of 3-4% for planned doses of 70-80Gy EQD2. CONCLUSION: TCP is robust to dosimetric uncertainties when dose prescription is in the more flat region of the dose-response curve at doses >75Gy...

  3. PATHOLOGICAL SUB TROCHANTERIC FRACTURE OF FEMUR FOLLOWING PELVIC IRRADIATION FOR CA CERVIX: A CASE REPORT

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    Shiva Naik

    2014-12-01

    Full Text Available : INTRODUCTION: Subtrochanteric fractures account for approximately 10-30% of all hip fractures, and they affect persons of all ages. Most frequently, these fractures are seen in 2 patient populations, namely older osteopenic patients after a low-energy fall and younger patients involved in high-energy trauma. A newer population of patients experience subtrochanteric fractures after bisphosphonate use. CASE PRESENTATION: A 45year old Indian woman presented at our orthopaedic outpatients department with 4 months history of pain in left hip and a 1 month history of inability to walk. She had pelvic irradiation for carcinoma of cervix 1 year earlier. Pelvic radiograph confirmed sub trochanteric fracture of left femur. CONCLUSION: Patients with hip pain who have been treated with pelvic irradiation should be thoroughly investigated for hip fractures.

  4. Rectal complication after remote afterloading intracavitary therapy for carcinoma of the uterine cervix

    Energy Technology Data Exchange (ETDEWEB)

    Teshima, T.; Chatani, M.; Hata, K.; Inoue, Ta.; Inoue, To.; Suzuki, T.

    1985-06-01

    From August 1978 through December 1980, 119 patients of previously untreated carcinoma of the uterine cervix were treated using RALS, remote afterloading high dose rate intracavitary therapy at our department. The data from 92 out of 119 patients were available for analysis of rectal complication. The incidence of major rectal complications was only 2% (2/92). Uni- and multivariate analyses were used based on the external criterion variable of rectal complication which included even minor injuries. By using these methods, it was clearly indicated that these factors such as TDF of rectum, Z-coordinate of weighted geometric center (WGC-Z), the dose of whole pelvic irradiation, history of chemotherapy and Treponema pallidum hemoagglutination test (TPHA) were important for occurrence of rectal complication. According to discriminant score, 71 out of 92 cases (77%) could be correctly discriminated.

  5. In vivo light scattering for the detection of cancerous and precancerous lesions of the cervix

    Energy Technology Data Exchange (ETDEWEB)

    Mourant, Judith R [Los Alamos National Laboratory

    2008-01-01

    A noninvasive optical diagnostic system for detection of cancerous and precancerous lesions of the cervix was evaluated in vivo. The optical system included a fiber-optic probe designed to measure polarized and unpolarized light transport properties of a small volume of tissue. An algorithm for diagnosing tissue based on the optical measurements was developed that used four optical properties, three of which were related to light scattering properties and the fourth of which was related to hemoglobin concentration. A sensitivity of {approx}77% and specificities in the mid 60% range were obtained for separating high grade squamous intraepithelial lesions and cancer from other pathologies and normal tissue. The use of different cross-validation methods in algorithm development is analyzed, and the relative difficulties of diagnosing certain pathologies are assessed. Furthermore, the robustness of the optical system for use by different doctors and to changes in fiber-optic probe are also assessed, and potential improvements in the optical system are discussed.

  6. Automated and interactive lesion detection and segmentation in uterine cervix images.

    Science.gov (United States)

    Alush, Amir; Greenspan, Hayit; Goldberger, Jacob

    2010-02-01

    This paper presents a procedure for automatic extraction and segmentation of a class-specific object (or region) by learning class-specific boundaries. We describe and evaluate the method with a specific focus on the detection of lesion regions in uterine cervix images. The watershed segmentation map of the input image is modeled using a Markov random field (MRF) in which watershed regions correspond to binary random variables indicating whether the region is part of the lesion tissue or not. The local pairwise factors on the arcs of the watershed map indicate whether the arc is part of the object boundary. The factors are based on supervised learning of a visual word distribution. The final lesion region segmentation is obtained using a loopy belief propagation applied to the watershed arc-level MRF. Experimental results on real data show state-of-the-art segmentation results on this very challenging task that, if necessary, can be interactively enhanced.

  7. Brachytherapy for stage IIIB squamous cell carcinoma of the uterine cervix: survival and toxicity

    Energy Technology Data Exchange (ETDEWEB)

    Zuliani, Antonio Carlos; Cunha, Maercio de Oliveira, E-mail: aczo.rt@gmail.co [Universidade Estadual de Campinas (UNICAMP), SP (Brazil); Esteves, Sergio C.B. [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Faculdade de Ciencias Medicas. Secao de Radioterapia; Teixeira, Julio Cesar [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Faculdade de Ciencias Medicas. Dept. de Tocoginecologia

    2010-07-01

    Objective: to compare survival and toxicity of three different treatments for stage IIIB cervix cancer: low-dose-rate (LDR), high-dose-rate (HDR) brachytherapy and association of HDR and chemotherapy. Methods: between 1985 and 2005, 230 patients with FIGO stage IIIB squamous cell carcinoma of the uterine cervix received 4-field pelvic teletherapy at doses between 40 and 50.4 Gy, with a different complementation in each group. The LDRB group, with 42 patients, received one or two insertions of LDR, with Cesium-137, in a total dose of 80 to 100Gy at point A. The HDR group, 155 patients received HDR in 4 weekly 7 Gy fractions and 9 Gy to 14.4 Gy applied to the involved parametria. The CHT group, 33 patients, were given the same treatment as the HDR group and received 5 or 6 weekly cycles of cisplatin, 40 mg per m2. Results: the five-year progression-free survival (PFS) was 60% for the HDR group and 45% for the LDR group, and the two-year PFS for the CHT group was 65% (p = 0.02). The five-year Overall Survival (OS) was 65% for the HDR group and 49% for the LDR group. The two-year OS was 86% for the CHT group (p 0.02). Rectum toxicity grade II was 7% for the LDR group, 4% for the HDR group and 7% for the CHT group that had one case of rectum toxicity grade IV. Conclusion: patients that received HDR had better OS and PFS. The Chemotherapy-HDR association showed no benefit when compared to HDR only. Toxicity rates showed no difference between the three groups. (author)

  8. Tumor regression dynamics with external radiotherapy in cancer cervix and its implications

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    Datta N

    2004-01-01

    Full Text Available BACKGROUND : To study the external radiotherapy (EXTRT regression patterns in cancer of the cervix. AIMS : Evaluate EXTRT tumor regression doses (TRD for 50% (TRD50, 80% response (TRD80, normalized dose response gradient (γ50 and slope (slope50 with clinical outcome. SETTINGS AND DESIGN : Patients, treated solely with radiotherapy and enrolled for other prospective studies having weekly tumor regressions recorded were considered. MATERIAL AND METHODS : Seventy-seven patients received 50Gy of EXTRT at 2 Gy/fraction followed by 18Gy of high-dose rate intracavitary brachytherapy at 6 Gy/fraction. Loco-regional regressions were assessed clinically at weekly intervals during EXTRT to generate EXTRT dose-response curves. STATISTICAL ANALYSIS USED : Student′s t test, logistic regression, Kaplan Meier and Cox′s proportional hazard model. Scatter plots were fitted using cubic fit. RESULTS : Age (P=0.052 and absence or presence of gross residual tumor (AGRT and PGRT respectively following EXTRT (P< 0.001 were the only determinants for complete response (CR at 1 month following completion of radiotherapy. EXTRT tumor regression sigmoid curves obtained for various patient characteristics differed only for those with AGRT and PGRT with differences in TRD50, (P< 0.001; TRD80 (P< 0.001 and slope50 (P=0.001. Response status to EXTRT was a prognosticator for loco-regional disease free survival (LDFS (AGRT vs. PGRT; P=0.046. On multivariate analysis, both TRD50 and TRD80 emerged as significant predictors for tumor status at end of EXTRT while TRD80 was the sole determinant of LDFS. CONCLUSION : Extent of tumor regression to EXTRT is an important predictor for treatment outcome in cancer cervix as evident from TRD50 and TRD80 values of EXTRT tumor regression curves.

  9. Priming effect of misoprostol on estrogen pretreated cervix in postmenopausal women.

    Science.gov (United States)

    Atmaca, Rusen; Kafkasli, Ayse; Burak, Feza; Germen, Aysegul Tezcan

    2005-07-01

    Misoprostol, which is a prostaglandin E1 analogue, is effectively used in cervical priming in women both for labor induction and for gynecological procedures. Although its efficacy is well documented in reproductive age women, during postmenopausal period this efficacy is limited probably due to estrogen deficit. Our objective is to evaluate if estrogen deficit in postmenopausal women is important for the effect of misoprostol on cervical ripening before diagnostic procedures. In this study, 45 patients were randomly allocated to estrogen or placebo group. The study group received local estrogen cream and other group received chlindamycine phosphate cream as placebo. The patients were given oral misoprostol 24 and 12 hours before the procedure for uterine cavity evaluation. Cervix was dilated by using Heagar dilator up to 6 mm. Data were analyzed by Student t-test, Mann-Whitney's U-test, chi-square test and paired samples t-test where appropriate. Basal cervical widths for the estrogen and placebo groups were 4.4 +/- 0.7 and 3.7 +/- 0.7 mm, respectively (p < 0.01). Mean time required for dilatation of cervix was 44.4 +/- 16.2 seconds for the estrogen group and 61.4 +/- 18.3 seconds for the placebo group (p < 0.01). As a conclusion, misoprostol treatment alone is not effective to get cervical priming in postmenopausal women, and as shown in our study, pretreatment with local estrogen overcome the failure. To get a beneficial effect of misoprostol on cervical ripening, estrogenic activity is necessary and when pretreated with local estrogen, misoprostol ameliorates cervical priming in postmenopausal women.

  10. Impact of MRI in the management and staging of cancer of the uterine cervix

    Energy Technology Data Exchange (ETDEWEB)

    Stenstedt, Kristina (Centre of Surgical Gastroenterology, Karolinska Univ. Hospital and Karolinska Inst., Stockholm (Sweden)); Hellstroem, Ann-Cathrin (Dept. of Gynecological Oncology, Radiumhemmet, Karolinska Univ. Hospital and Karolinska Inst., Stockholm (Sweden)); Fridsten, Susanne; Blomqvist, Lennart (Dept. of Diagnostic Radiology Karolinska Univ. Hospital and Karolinska Inst., Stockholm (Sweden))

    2011-04-15

    Background. Cervical carcinoma is the only gynecological tumor still being staged mainly by clinical examination and only a limited use of diagnostic radiology. Cross sectional imaging is increasingly used as an aid in the staging procedure. We wanted to assess the impact of magnetic resonance imaging (MRI) in addition to the clinical staging of patients with carcinoma of the uterine cervix. Material and methods. A retrospective single-centre analysis of 183 women referred to a tertiary referral centre for gynecological tumors (<= 65 years old) with cervical cancer diagnosed between January 1, 2003 and December 31, 2006 who have undergone an MRI investigation before start of treatment. Patient records were retrospectively reviewed and any change of the planned treatment after the MRI examination was noted. Results. In patients with cervical carcinoma FIGO stage Ia2-IIa treated surgically, the treatment plan was altered due to MRI results in 10/125 patients. In the smaller group of patients with clinically more advanced disease receiving radio-chemotherapy, the treatment plan was altered in 12/58 patients. Reasons for changing the treatment plan after MRI were findings indicating a higher (n = 8) or lower (n = 5) local tumor stage, findings of para aortic nodal disease (n = 4) or difficulty to clinically examine the patient due to obesity (n = 2). MRI was also an aid in deciding whether or not to offer fertility preserving treatment in three cases. Conclusion. The use of MRI affects treatment planning in patients with cancer of the uterine cervix. The impact is more obvious in more advanced stages of disease and in patients who are difficult to examine clinically due to, for example body constitution. The result of MRI is also an aid in deciding whether or not a fertility preserving operation is feasible

  11. [Cervix priming in induced abortion in the 1st trimester using intracervical administration of sulprostone gel].

    Science.gov (United States)

    Rath, W; Meyer, D; Harder, D; Hilgers, R; Kuhn, W

    1985-01-01

    In a prospective, randomised study 40 primigravidae were treated intracervically with 0.05 mg or 0.1 mg Sulprostone-Tylose gel in order to soften the cervix prior to first trimester termination of pregnancy. Curettage was performed on the average 7.5 hours after prostaglandin administration. For objective demonstration of the priming effect, the force required for dilatation of the cervical canal was measured in Newtons by a special tonometer before prostaglandin application and before operation. The maximal dilatability with a force of 10 N, the increase in dilatability after local PG application, and the patency of the cervix were measured. The occurrence of PG-induced lower abdominal pain associated with contractions was analysed with regard to the number of episodes at pain, their timing and the required amount of analgesics. A modified visual analogue scale was used to evaluate the subjective pain experience. The abortive effect of 0.1 mg Sulprostone was found to be more efficient than the 0.05 mg dose. There was no statistical significant difference between the two doses, however, for the priming effect detectable with the tonometer. The subjective experience of pain, use of analgesics and the frequency of gastrointestinal side effects were significantly higher with 0.1 mg than with 0.05 mg Sulprostone. The visual analogue scale allows the patient to quantify, at least to some extent, her experience of pain, and enables a differentiated analgetic therapy. Because of its effectiveness and low rate of side effects, the intracervical application of 0.05 mg Sulprostone gel promises to be an advantageous alternative to other methods of cervical priming.

  12. Analysis of Radiotherapy Associated Factors in Stage IIb Carcinoma of Uterine Cervix

    Energy Technology Data Exchange (ETDEWEB)

    Moon, Chang Woo; Jeung, Tae Sig; Yum, Ha Yong [Kosin Medical College of Medicine, Pusan (Korea, Republic of)

    1990-12-15

    331 patients of stage IIb uterine cervix cancer treated by radiation alone at Kosin Medical Center between June 1980 and Dec. 1985 were analysed to determine parameters of radiotherapy associated to disease states. Survival rate was highest among the reported (82.8% for crude and 82.4% for disease free survival). Pelvic control rate in 6 weeks after the end of radiotherapy was 93.6% in the patients treated with ICR following total pelvic radiation and 71.6% with small field additional external irradiation. 5 year survival rate in those who achieved pelvic control was 98. 9% and 12.9% in those who had pelvic failure and/or metastasis after radiation. The survival rate figured maximal 88.5% with dosage of 7500-8500 cGy to point A with acceptable incidence of complications (4.9%) but without increasing survival above it and minimal 74.1% with dosage of less than 6500 cGy. The treatment failure was counted 18.7% (62 of 331 patients): Local failure 72. 6% ( 45 of 62 patients), locoregional failure 3.2% (2 of 62 patients) and distant failure 24% (15 of 62 patients). Late complications were found In 50 patients (15.1%) and 42% of them was rectal bleeding and stenosis. The dose of 8500 cGy to point A was found to be critical for complication and 70% of complications occurred above it and was more serious one such as fistula. Rectal complications were developed above rectal dose 6500 cGy and bladder complication above bladder dose 7500 cGy. Major cause of death was cachexia due to locoregional failure (73.7% of death), next was due to metastasis to lung, liver and bone, and only 3 patients died of complication of intestinal perforations and obstruction. In conclusion higher external radiation dose for a bulky uterine cervix and barrel shaped uterus was essential for local control.

  13. 传统涂片与液基薄层细胞制片在宫颈脱落细胞学中的应用比较%The Comparison of Traditional pap Smear and Liqud-based Cytology in Cervix Uteri Cytology

    Institute of Scientific and Technical Information of China (English)

    廖绍君

    2013-01-01

      目的对传统涂片和液基薄层细胞制片在宫颈脱落细胞学中的应用情况进行比较,以寻求筛查宫颈癌和癌前病变的最好方法。方法对2007年3月至2011年10月在我院行传统涂片的4513例和3777例行液基薄层细胞制片的体检人群的结果进行比较。细胞学诊断采用 TBS(the Bethesda system)分类诊断系统。结果液基层细胞学在良性改变和正常、诊断意义不明的不典型的鳞状上皮细胞(ASCUS,atypical squamous cels of undetermined significance)、低度鳞状上皮内病变(LSIL,low-grade squamous intraepithelial lesion)及以上病变的诊断方面优于传统涂片,对微生物的总体检出率不如传统涂片,但对滴虫、人类乳头瘤病毒(HPV, human papilo ma virus)和疱疹病毒(HSV, Herpesvirus hominis)的检测方面优于传统涂片。结论液基薄层细胞学在筛查宫颈癌和癌前病变上优于传统涂片。%Objective To find the better method to detect cervical cancer and precancerous lesions through the comparison of traditional pap smear and liqud-based cytology. Methods 4513 cases using traditional pap smear and 3777 cases using liqud-based cytology were analysed in normal people. The Bethesda system was used to diagnose. Results The diagnosis of ASCUS,LSIL and higher grade lesions in liqud-based cytology is better than traditional pap smear. The total detection rate of microorganism is lower, but the detection rate of trichomoniasis, HPV and HSV is higher. Conclusions In detecting cervical cancer and precancerous lesions, liqud-based cytology is better than traditional pap smear.

  14. Can pure accelerated radiotherapy given as six fractions weekly be an option in locally advanced carcinoma cervix: Results of a prospective randomized phase III trial

    Directory of Open Access Journals (Sweden)

    Mukesh Sharma

    2016-01-01

    Conclusions: Accelerated radiotherapy given as six fractions per week is an effective alternative to concomitant chemoradiation in locally advanced carcinoma cervix and has shown lesser toxicities in our study.

  15. Effects of depomedroxyprogesterone acetate on the development and maintenance of Candida albicans in the vagina of oophorectomized Wistar rats (Rattus norvegicus

    Directory of Open Access Journals (Sweden)

    Agenor Storti-Filho

    2011-03-01

    Full Text Available The objective of this study was to determine the effect of medroxyprogesterone acetate (DMPA on the development and maintenance of Candida albicans in the vagina of oophorectomized Wistar rats. The animals were divided into negative control groups (NCG, which received injections of sterile saline; positive control groups (PCG, which were given injections of estradiol valerate; and progesterone groups (PG, which were given injections of Depo-Provera®. After one week of hormonal induction, vaginal infection by C. albicans was induced in all the groups and detected by vaginal yeast culture and Papanicolaou smear. In addition, scanning and transmission electron microscopy images were obtained to confirm the vaginal infection by yeast in PG. A difference in progesterone levels in PG was observed between the basal level and after hormonal induction (PO objetivo do presente estudo foi determinar os efeitos do acetato de depomedroxyprogesterona (ADMP no desenvolvimento e manutenção de Candida albicans na vagina de ratas Wistar ooferectomizadas. Os animais foram divididos em grupos controle negativos (GCN, que receberam injeções de salina estéril; grupos controle positivos (GCP, que receberam injeções de valerato de estradiol; e grupos progesterona (GP, nos quais foram feitas injeções de Depo-Provera®. Após uma semana da aplicação hormonal, foi induzida a infecção vaginal por C. albicans em todos os grupos, detectada por cultura para leveduras vaginais e esfregaço de Papanicolaou. Foram feitas ainda imagens por microscopia eletrônica de varredura e transmissão para confirmar a infecção pela levedura no GP. Foram observados diferentes níveis de progesterona em GP, entre os valores basais e após a indução hormonal (P<0,0001. Neste grupo, 100,0% das ratas contraíram a infecção vaginal na primeira semana, mas não a mantiveram até a terceira semana. A forma farmacêutica de ADMP foi efetiva em induzir as fases de metaestro e

  16. Literature analysis of radiotherapy in uterine cervix cancer for the processing of the patterns of care study in Korea

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Doo Ho; Kim, Eun Seog; Kim, Yong Ho [College of Medicine, Soonchunhyang University, Seoul (Korea, Republic of); Kim, Jin Hee [College of Medicine, Keimyung University, Daegu (Korea, Republic of); Yang, Dae Sik [College of Medicine, Korea University, Seoul (Korea, Republic of); Kang, Seung Hee [College of Medicine, Ajou University, Suwon (Korea, Republic of); Wu, Hong Gyun; Kim, Il Han [College of Medicine, Seoul National University, Seoul (Korea, Republic of)

    2005-06-15

    Uterine cervix cancer is one of the most prevalent women cancer in Korea. We analysed published papers in Korea with comparing Patterns of Care Study (PCS) articles of United States and Japan for the purpose of developing and processing Korean PCS. We searched PCS related foreign-produced papers in the PCS homepage (212 articles and abstracts) and from the Pub Med to find Structure and Process of the PCS. To compare their study with Korean papers, we used the internet site 'Korean Pub Med' to search 99 articles regarding uterine cervix cancer and radiation therapy. We analysed Korean paper by comparing them with selected PCS papers regarding Structure, Process and Outcome and compared their items between the period of before 1980's and 1990's. Evaluable papers were 28 from United States, 10 from the Japan and 73 from the Korea which treated cervix PCS items. PCS papers for United States and Japan commonly stratified into 3 {approx} 4 categories on the bases of the scales characteristics of the facilities, numbers of the patients, doctors. Researchers restricted eligible patients strictly. For the process of the study, they analysed factors regarding pretreatment staging in chronological order, treatment related factors, factors in addition to FIGO staging and treatment machine. Papers in United States dealt with racial characteristics, socioeconomic characteristics of the patients, tumor size (6), and bilaterality of parametrial or pelvic side wall invasion (5), whereas papers from Japan treated of the tumor markers. The common trend in the process of staging work-up was decreased use of lymphangiogram, barium enema and increased use of CT and MRI over the times. The recent subject from the Korean papers dealt with concurrent chemoradiotherapy (9 papers), treatment duration (4), tumor markers (8) and unconventional fractionation. By comparing papers among 3 nations, we collected items for Korean uterine cervix cancer PCS. By consensus meeting

  17. Analysis of microbial flora in dairy cow's vagina, and the isolation and identification of lactobacillus%奶牛阴道菌群分析与乳酸菌的分离及鉴定

    Institute of Scientific and Technical Information of China (English)

    侯荣伦; 程超; 付艳茹; 刘彦民; 李利平; 郝永清; 黄少磊

    2011-01-01

    目的 研究奶牛阴道菌群,并从健康奶牛阴道分离出产酸能力很强的乳酸菌.方法 采用常规的方法对奶牛阴道进行细菌的分离及鉴定,并进行菌群分析.结果 健康奶牛阴道优势菌群主要为乳酸菌(P<0.01),屡配不孕奶牛阴道优势菌群主要为金黄色葡萄球菌(P<0.01);从健康奶牛阴道分离出的乳酸菌为55株,其中产酸能力很强的6株乳酸菌鉴定结果分别为Lactobacillus plantarum,Lactobacilluz brevis,Enterococcus faecalis,Lactococcus garvieae、Lactobacillus kitasatonis和Lactobacillus amylovorus.结论 奶牛阴道菌群中分离的6株乳酸菌可作为潜在的奶牛阴道微生态制剂进行深入研究.%Objective To Analyse the microbial flora in dairy cow's vagina, isolate and identify Lactobacillus with strong acid-producing capacity from vagina of healthy dairy cows. Method The standard microbiological methods were used to isolate and identify the bacteria in dairy cow's vagina. Result The most dominant bacteria in the vagina of healthy dairy cows belonged to Lactobacillus( P <0.01 ); The most dominant bacteria in the vagina of infertile dairy cows belonged to Staphylococcus aureus(P < 0.01 ); 55 lactic acid bacteria were isolated from the vagina of healthy dairy cows. 6 of which with strong acid-producing capacity were further identified as Lactobacillus plantarum, Lactobacillus brevis, Enterococcus faecalis, Lactococcus garvieae, Lactobacillus kitasatonis and Lactobacillus amylovorus, respectively. Conclusion The 6 lactic acid bacteria strains can be used as the candidates for the preparation of microecological additives of dairy cows and need further study.

  18. Inhibition of HIV-1 infection in ex vivo cervical tissue model of human vagina by palmitic acid; implications for a microbicide development.

    Directory of Open Access Journals (Sweden)

    Xudong Lin

    Full Text Available BACKGROUND: Approximately 80% of all new HIV-1 infections are acquired through sexual contact. Currently, there is no clinically approved microbicide, indicating a clear and urgent therapeutic need. We recently reported that palmitic acid (PA is a novel and specific inhibitor of HIV-1 fusion and entry. Mechanistically, PA inhibits HIV-1 infection by binding to a novel pocket on the CD4 receptor and blocks efficient gp120-to-CD4 attachment. Here, we wanted to assess the ability of PA to inhibit HIV-1 infection in cervical tissue ex vivo model of human vagina, and determine its effect on Lactobacillus (L species of probiotic vaginal flora. PRINCIPAL FINDINGS: Our results show that treatment with 100-200 µM PA inhibited HIV-1 infection in cervical tissue by up to 50%, and this treatment was not toxic to the tissue or to L. crispatus and jensenii species of vaginal flora. In vitro, in a cell free system that is independent of in vivo cell associated CD4 receptor; we determined inhibition constant (Ki to be ∼2.53 µM. SIGNIFICANCE: These results demonstrate utility of PA as a model molecule for further preclinical development of a safe and potent HIV-1 entry microbicide inhibitor.

  19. A vagina pós-orgânica: intervenções e saberes sobre o corpo feminino acerca do “embelezamento íntimo”

    Directory of Open Access Journals (Sweden)

    Marcelle Jacinto da Silva

    Full Text Available Resumo Este trabalho tem como objetivo problematizar textos disponíveis na internet favoráveis aos procedimentos de embelezamento íntimo, associados mais aos argumentos sobre autoestima e saúde da mulher e menos à vaidade. Há uma relação direta entre saúde da mulher e embelezamento da região íntima/aparência da genitália, mas também se fala que as cirurgias não ocorrem para corrigir apenas a estética/aparência da região íntima, que inicialmente eram cirurgias para a correção de problemas funcionais. Nesse contexto, a “mulher moderna” seria aquela que aproveita as novidades tecnológicas para proveito próprio – no entanto, há muitas controvérsias, nomenclaturas e significados em jogo. Se a vagina tem sido alvo da “ditadura da beleza” tanto quanto outras partes do corpo feminino, e se há possibilidade de fabricá-la, é possível pensá-la enquanto pós-orgânica. Eis o argumento que guia este artigo, acionando concepções referentes a corpo, gênero, sexualidade, beleza e saúde.

  20. Pemphigus vulgaris of the vagina--its cytomorphologic features on liquid-based cytology and pitfalls: case report and cytological differential diagnosis.

    Science.gov (United States)

    Onuma, Kazuya; Kanbour-Shakir, Amal; Modery, Judith; Kanbour, Anisa

    2009-11-01

    Pemphigus vulgaris is an autoimmune bullous disease that involves skin and mucous membrane. Although the female genital tract is rarely affected, its cytomorphologic features on a conventional cervicovaginal smear have been notoriously described with a possibility of being misdiagnosed as neoplastic lesions. Its cytological mimicry for squamous cell carcinoma has been described as having serious misdiagnostic potential, particularly if appropriate clinical information is not provided. We report a case of pemphigus vulgaris involving the vagina diagnosed on a liquid-based cervicovaginal smear Thin Prep(R) (TP). The smear was initially reported as squamous cell carcinoma. However, re-evaluation of the TP smear raised the suspicion of pemphigus, which was supported subsequently by clinical information and tissue biopsy confirmation. We described the cytologic features of pemphigus vulgaris on a TP smear and compared them with those of neoplastic conditions. Pemphigus cells on a TP smear exhibit similar cytomorphologic features to those on a conventional smear. Recognizing euchromatin pattern and smooth nuclear contours which may be better appreciated on a TP can prevent overdiagnosis.

  1. Successful Treatment of a Granulocytic Sarcoma of the Uterine Cervix in Complete Remission at Six-Year Follow-Up

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    Stefano C. H. Kim

    2010-01-01

    Full Text Available Background. Localized granulocytic sarcoma of the uterine cervix in the absence of acute myelogenous leukemia (AML at presentation is very rare, its diagnosis is often delayed, and its prognosis almost always ominous evolving into refractory AML. Case. We present the case of a 30-year-old woman with vaginal bleeding and a large cervical mass. Further evaluation confirmed the presence of a granulocytic sarcoma but failed to reveal systemic involvement. Results. AML type chemotherapy followed by radiotherapy of the uterus led to a durable complete remission. She remains in complete remission six years after diagnosis. Conclusion. Granulocytic sarcoma of the cervix is a rare entity for which early intensive AML type therapy is effective.

  2. Glassy cell carcinoma of the uterine cervix a rare histology. Report of three cases with a review of the literature

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    Deshpande Archana

    2004-01-01

    Full Text Available Glassy cell carcinoma is a poorly differentiated variant of adenosquamous carcinoma of the cervix associated with an aggressive course and a poor prognosis. We present three cases of glassy cell carcinoma of the cervix. Patients presented with a cervical growth which was biopsied. Histology the tumours showed nests of cells with a granular or clear cytoplasm, displaying marked pleomorphism and mitoses. Stroma showed an eosinophilic infiltrate. Two tumours showed a pure glassy cell pattern and one showed glandular differentiation with intracellular and extracellular mucin. Patients were treated with a combination of surgery, radiotherapy and chemotherapy but showed a poor response. Two patients died of the disease of pelvic or distant metastases within two years of diagnosis and one was lost to follow up. Although glassy cell carcinoma runs an aggressive clinical course, an early diagnosis may help in a more effective management and offer a better prognosis.

  3. F-18 FDG PET Images of the Cervix at Various Time Points after the Loop Electrosurgical Excision Procedure

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    Jeong, Shin Young [Kyungpook National University Medical School, Daegu (Korea, Republic of); Min, Jung Joon [Chonnam National University Medical School, Gwangju (Korea, Republic of)

    2010-04-15

    F-18 FDG PET is useful for monitoring residual or recurrent tumors after surgical resection. We describe five F-18 FDG PET images of three patients who had cervical carcinoma and then underwent a loop electro surgical excision procedure (LEEP). Two of the images were taken within 15 days and three at least 2 months after LEEP. The earlier F-18 FDG PET images revealed linear hypermetabolic lesions in the cervix that were produced by inflammation. This was confirmed by pathological analysis. The later F-18 FDG PET images did not reveal any remarkable hypermetabolism in the cervix without any treatment. These observations suggest that, to determine the response to LEEP therapy, F-18 FDG PET should not be performed within 15 days of the procedure.

  4. Small Cell Carcinoma of the Uterine Cervix in a Pregnant Patient Diagnosed with Liquid Based Cytology and Cell Block Immunocytochemistry

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    Mawuli F. Attipoe

    2014-01-01

    Full Text Available Definitive cytomorphologic diagnosis of small cell carcinoma of the uterine cervix is possible but can be challenging in routine cervicovaginal cancer screening specimens. Several small series of reported cases of cervical small cell carcinoma have shown this uncommon malignancy to represent fewer than 2% of all invasive cervical cancers. This tumor type is associated with poor prognosis and rapid disease progression and can develop to an advanced stage in the interval between screening visits. Only rare case reports of small cell carcinoma arising in gravid cervices are known. In the current case a 29-year-old, gravida 6, para 2, pregnant (10-week gestation female presented with postcoital bleeding. A definitive diagnosis of small cell carcinoma of the cervix was made possible by liquid based Pap testing with ancillary cell block preparation allowing for immunocytochemical characterization of the lesional cell population.

  5. Analysis on the Incidence, Staging and Treatment of Carcinoma Cervix at Delta Medical College and Hospital of Bangladesh

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    Parvin A Banu

    2013-07-01

    Full Text Available Background: Cervical cancer remains the most common cancer among women in this part of the world. Bangladesh has the highest level of incidence and mortality rates due to cervical cancer among women. Cervical cancer is a preventable disease by screening and treatment of pre-invasive condition. Unfortunately, there is no effective screening program in Bangladesh.Objective: The objective was to analyze the clinico-pathologic characteristics and, subsequently the therapy delivered to the patients.Materials and method: This cross sectional study was done from January to December 2011 in oncology division of Delta Medical College and Hospital, Dhaka, Bangladesh. During this period a total of 2264 female carcinoma patients were registered. Out of them 523 patients were with the diagnosis of carcinoma of cervix. Evaluation and characterization of patients with carcinoma cervix were done according to the age group, clinical stages and surgical status. External beam radiotherapy (EBRT and doses, brachytherapy doses and fractions, time interval between EBRT and brachytherapy, neo-adjuvant and concurrent chemotherapy status were the factors taken into consideration for the analysis of treatment of these patients.Results: The study revealed that the incidence of carcinoma cervix was 23% and highest incidence was between 40-50 years of age group, most of the patients were in Stage IIB (34% and IIIB (28% and 31% with unknown stage. About 44% patients were referred postoperatively and most of them with unknown stage. Almost 90% patients received EBRT, 24% patients received concurrent chemo-radiation, 46% of them completed 5 cycles of concurrent chemotherapy and 8.6% patients received neo-adjuvant chemotherapy. About 69% patients received brachytherapy with HDR Co-60 sources and 23% of patients received their brachytherapy treatment after 12 weeks of completion of EBRT.Conclusion: Lack of proper clinical evaluation and documentation, delay in referral and lack

  6. Treatment results of radical radiotherapy of carcinoma uterine cervix using external beam radiotherapy and high dose rate intracavitary radiotherapy

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    Azad S

    2010-01-01

    Full Text Available Aim: To report the outcome of carcinoma cervix patients treated radically by external beam radiotherapy and high dose rate intracavitary radiotherapy. Material and Methods: From January 2005 to December 2006, a total of 709 newly diagnosed cases of carcinoma cervix were reported in our department. All cases were staged according to the International Federation of Gynecologist and Oncologist staging system. Out of 709 cases, 342 completed radical radiotherapy and were retrospectively analyzed for the presence of local residual disease, local recurrence, distant metastases, radiation reaction, and disease free survival. Results: There were 11(3.22%, 82(23.98%, 232(67.83%, and 17(4.97% patients in stages I, II, III, and IV, respectively. The median follow up time for all patients was 36 months (range 3 -54 months. The overall treatment time (OTT ranged from 52 to 69 days (median 58 days. The 3 year disease free survival rate was 81.8%, 70.7%, 40.08%, and 11.76% for stages I, II, III, and IV, respectively. There were 91 (26.6% cases with local residual diseases, 27(7.9% developed distant metastasis, and 18(5.26% pts had local recurrence. Discussion: The results of this study suggest that radical radiotherapy with HDR brachytherapy was appropriate for the treatment of early staged cancer of uterine cervix. For locally advanced cancer of cervix addition of concurrent chemotherapy, higher radiation doses, reduction of overall treatment time to less than 8 weeks, and use of latest radiotherapy techniques such as IMRT is recommended to improve the results.

  7. Dose verification in carcinoma of uterine cervix patients undergoing 3D conformal radiotherapy with Farmer type ion chamber

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    Challapalli Srinivas

    2014-01-01

    Full Text Available External beam radiotherapy (EBRT for carcinoma of uterine cervix is a basic line of treatment with three dimensional conformal radiotherapy (3DCRT in large number of patients. There is need for an established method for verification dosimetry. We tried to document absorbed doses in a group of carcinoma cervix patients by inserting a 0.6 cc Farmer type ion chamber in the vaginal cavity. A special long perspex sleeve cap is designed to cover the chamber for using in the patient′s body. Response of ionization chamber is checked earlier in water phantom with and without cap. Treatment planning was carried out with X-ray computed tomography (CT scan and with the chamber along with cap in inserted position, and with the images Xio treatment planning system. Three measurements on 3 days at 5-6 fraction intervals were recorded in 12 patients. Electrometer measured charges are converted to absorbed dose at the chamber center, in vivo. Our results show good agreement with planned dose within 3% against prescribed dose. This study, is a refinement over our previous studies with transmission dosimetry and chemicals in ampules. This preliminary work shows promise that this can be followed as a routine dose check with special relevance to new protocols in the treatment of carcinoma cervix with EBRT.

  8. Influence of breed and age on morphometry and depth of inseminating catheter penetration in the ewe cervix: a postmortem study.

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    Kaabi, M; Alvarez, M; Anel, E; Chamorro, C A; Boixo, J C; de Paz, P; Anel, L

    2006-11-01

    A detailed examination of the cervical canal in the ewe was carried out. This analysis could be used to design new catheters for artificial insemination (AI) to achieve deeper cervical penetration and therefore better fertility results. Three hundred and sixty-five cervices from four sheep breeds (Churra, Assaf, Merino, Castellana) obtained postmortem were used. Cervix morphometry and depth of cervical penetration using two types of catheters were determined. A conventional straight catheter for ovine artificial insemination (IMV), and a bent catheter, ending in a stainless steel needle, 9 cm in length and with an 8 mm tip bent 45 degrees , were used. The results showed that the morphometry of the cervix depends on breed and age of the ewe. The cervices of Churra breed were shorter and narrower, and had a higher number of folds than those of other breeds. Postmortem cervical penetration was deeper when the cervices were longer and wider, and with fewer folds (Merino and Castellana breeds). In ageing ewes, the cervix tended to become longer and wider, with loose folds. This decreased structural complexity and significantly improved cervical penetration. The bent catheter allowed significantly greater cervical penetration than the straight IMV one.

  9. [Facilitating cervix dilatation of the non-pregnant uterus by intracervical administration of gels containing prostaglandin and calcium chloride].

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    Rath, W; Hüther, G; Hilgers, R; Meyer, A; Kuhn, W; Neuhoff, V

    1987-01-01

    In a prospective, randomised study, 50 non-pregnant patients were treated intracervically with 3 ml 5% tylose, 50 micrograms sulprostone, 100 micrograms sulprostone gel, 3 ml 2.5 mM or 9.0 mM calcium chloride gel in order to soften the cervix 12-14 hours before diagnostic curettage. The gel was not used in a further 20 patients. To objectively demonstrate the priming effect, the force required for dilatation of the cervical canal was measured in Newtons, using a mechanical tonometer both before gel application and before the operation. In comparison with the administration of tylose only, the intracervical application of either sulprostone gel or calcium chloride gel led to a significant improvement in cervical dilatability. Tylose alone had a slight but measurable effect on the cervix. An increase in sulprostone from 50 micrograms to 100 micrograms or calcium molarity from 2.5 mM to 9.0 mM brought no further improvement in the dilatory effect. Dilatation-induced cervical lesions could be avoided by preoperative cervical ripening. After application of sulprostone, 3 out of 20 patients experienced doses-dependent uterine cramps, while all patients treated with calcium chloride gel were free of side effects. The intracervical administration of sulprostone and calcium chloride gel allowed gentle dilatation of the non-pregnant cervix, thus lowering the risk of uterine lesions. Under clinical aspects, cervical priming facilitates diagnostic and therapeutic procedures, which, in exceptional cases, can be performed without anaesthesia.

  10. Status of carcinoma cervix and high risk HPV 16 DNA in women with postmenopausal uterine bleeding (PMB

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    Veena Kashyap

    2014-09-01

    Full Text Available Postmenopausal bleeding (PMB is a discharge that occurs following the firm diagnosis of menopause, which is at least six months from the end of women’s menstrual cycle but not to be confused with infrequent or irregular periods occurring around the time of menopause. It is a common problem representing 5% of all gynecology outpatient attendances which are to eliminate endometrial cancer as the cause of bleed and PMB should be reported urgently to the gynecologist. Uterine bleeding in postmenopausal women is highly indicative clinically of malignancy originating from cervix or endometrium and Human papilloma virus (HPV is one of the causative agent for carcinoma cervix. Incidence of carcinoma cervix increases with the age in mature women, however, incidence of human papillomavirus (HPV infection reduces as menopause sets in. The presence of the virus could be used as an early indication of disease potential. Because the Pap test can only detect clinical evidence of cervical disease, molecular-based diagnostic tools are being used more frequently to detect the virus before abnormal cell growth can be observed. This study was aimed to determine the status of cervical cancer and HPV 16 DNA positivity in relation to postmenopausal bleeding.

  11. Individual and combined effects of relaxin, estrogen, and progesterone in ovariectomized gilts. I. Effects on the growth, softening, and histological properties of the cervix.

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    Winn, R J; Baker, M D; Sherwood, O D

    1994-09-01

    Marked growth and softening of the uterine portion of the cervix occur during the last third of the 115-day gestation period in the gilt. These changes in the cervix are temporally correlated with elevated blood levels of relaxin, estrogen, and progesterone. We recently demonstrated that relaxin plays a major role in promoting both the growth and softening of the cervix that occur in pregnant gilts. The roles of estrogen and progesterone in these cervical changes remain poorly understood. Accordingly, this study determined the influence of relaxin, estrogen, and progesterone, individually and in combination, on cervical growth and softening in gilts. Fifteen days after ovariectomy, six to nine nonpregnant, sexually mature gilts were assigned to one of the following eight treatment groups: ovariectomized controls, relaxin treated, estrogen treated, progesterone treated, estrogen plus relaxin treated, progesterone plus relaxin treated, estrogen plus progesterone treated, and progesterone plus estrogen plus relaxin treated. Treatment was given for 10 days, with doses of relaxin (0.5 mg, four times daily), estradiol benzoate (1 mg, twice daily), and progesterone (50 mg, twice daily) selected to provide blood levels resembling those between days 100-110 of gestation. The growth, softening, and histological characteristics of the cervices were determined. Treatment with relaxin significantly increased the growth and softening and altered the histological characteristics of the uterine portion of the cervix in the absence of steroid treatment. Estrogen treatment alone increased cervical growth, but when given in combination with relaxin, estrogen did not augment relaxin's ability to increase either cervical growth or softening. Progesterone alone had little or no effect on the growth or softening of the uterine portion of the cervix. Unexpectedly, when given in combination with relaxin, progesterone augmented markedly relaxin's effects on softening and alteration of the

  12. Clear cell carcinoma of the uterine cervix: clinical characteristics and feasibility of fertility-preserving treatment

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    Jiang X

    2014-01-01

    Full Text Available Xiang Jiang, Ying Jin, Yan Li, Hui-Fang Huang, Ming Wu, Keng Shen, Ling-Ya Pan Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People's Republic of China Abstract: The objective of this retrospective study was to analyze the clinical characteristics and prognosis of clear cell adenocarcinoma (CCA in the post-diethylstilbestrol (DES era and to evaluate the feasibility of fertility-preserving treatment. The records of 32 patients with CCAs who were treated at Peking Union Medical College Hospital from August 1986 to June 2012 were retrospectively reviewed. Three of the patients had undergone fertility-preserving treatment. The incidence of CCA among cervical adenocarcinomas was 15.2%. The median age was 38 years: 11 patients (34.4% were diagnosed before 30 years of age and two (6.3% after 70 years of age. Ten patients (31.2% were nulliparous. No patient had been exposed to DES. Twenty-nine patients (90.6% presented with obvious symptoms, and the cervix appeared abnormal in 26 patients (81.3%. Cervical Papanicolaou (Pap tests were abnormal in all four patients in whom they were performed (three had high-grade squamous intraepithelial lesions and one had atypical squamous cells of undetermined significance. The distribution by stage was 56.3% stage I, 34.4% stage II, 6.3% stage III, and 3.1% stage IV. Treatments mainly included surgery for patients with stage I to IIA CCA and radiochemotherapy for patients with advanced CCA. The overall 5-year progression-free survival was 72.2%. Patients with stage I to IIA CCA had better 5-year progression-free survival than did patients with stage IIB to IV CCA (81.5% versus 40.0%, P=0.003. The three patients who had undergone fertility-preserving treatment had no recurrences. CCA may also affect adolescents and children without prior DES exposure, who are often misdiagnosed as having functional uterine

  13. Detection of the Human 70-kD and 60-kD Heat Shock Proteins in the Vagina: Relation to Microbial Flora, Vaginal pH, and Method of Contraception

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    P. Giraldo

    1999-01-01

    Full Text Available The expression of the 60-kD and 70-kD heat shock proteins (hsp60 and hsp70 in the vaginas of 43 asymptomatic women of reproductive age with or without a history of recurrent vulvovaginitis (RVV were compared. Vaginal wash samples were obtained and assayed by enzyme-linked immunosorbent assay (ELISA for human hsp60 and hsp70. Heat shock protein 70 was not detected in any of the 19 women with no history of RVV, and hsp60 was present in only one woman in this group. In contrast, in the RVV group, 11 (45.8% were hsp60-positive and eight (33.3% were hsp70-positive. The presence of either heat shock protein in the vagina was associated with an elevated vaginal pH (>4.5. Bacterial vaginosis or Candida was identified in some of the asymptomatic subjects; their occurrence was significantly higher in women with vaginal hsp70 than in women with no heat shock proteins. Oral contraceptives were used by 35.7% of subjects who were negative for vaginal heat shock proteins, as opposed to only 12.5% of women who were positive for hsp70 and 8.3% who were positive for hsp60. Expression of heat shock proteins in the vagina may indicate an altered vaginal environment and a susceptibility to vulvovaginal symptoms. Infect. Dis. Obstet. Gynecol. 7:23–25, 1999.

  14. Incidence and mortality rates in breast, corpus uteri, and ovarian cancers in Poland (1980–2013: an analysis of population-based data in relation to socio-economic changes

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    Banas T

    2016-09-01

    Full Text Available Tomasz Banas,1 Grzegorz Juszczyk,2 Kazimierz Pitynski,1 Dorota Nieweglowska,1 Artur Ludwin,1 Aleksandra Czerw2 1Department of Gynecology and Oncology, Jagiellonian University Medical College, Krakow, 2Faculty of Health Science, Medical University of Warsaw, Warsaw, Poland Objectives: This study aimed to analyze incidence and mortality trends in breast cancer (BC, corpus uteri cancer (CUC, and ovarian cancer (OC in Poland in the context of sociodemographic changes.Materials and methods: Incidence and mortality data (1980–2013 were retrieved from the Polish National Cancer Registry, while socioeconomic data (1960–2013 were obtained from the World Bank. Age-standardized incidence and mortality rates were calculated by direct standardization, and join-point regression was performed to describe trends using the average annual percentage change (AAPC.Results: A significant decrease in birth and fertility rates and a large increase in gross domestic product were observed together with a decrease in the total mortality rate among women, as well as an increase in life expectancy for women. A large, significant increase in BC incidence was observed (AAPC1980–1990 2.14, AAPC1990–1996 4.71, AAPC1996–2013 2.21, with a small but significant decrease in mortality after a slight increase (AAPC1980–1994 0.52, AAPC1994–2013 −0.66. During the period 1980–2013, a significant increase in CUC incidence (AAPC1980–1994 3.7, AAPC1994–2013 1.93 was observed, with an initial mortality-rate reduction followed by a significant increase (AAPC1980–2006 −1.12, AAPC2006–2013 3.74. After the initial increase of both OC incidence and mortality from 1994, the incidence rate decreased significantly (AAPC1980–1994 2.98, AAPC1994–2013 −0.49, as did the mortality rate (AAPC1980–1994 0.52, AAPC1994–2013 −0.66.Conclusion: After 1994, a decrease in OC incidence was found, while the incidence of BC and CUC continued to increase. A reduction in

  15. Chromosomal Radiosensitivity in Lymphocytes of Cervix Cancer Patients—Correlation with Side Effect after Radiotherapy

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    Wegierek-Ciuk, Aneta; Lankoff, Anna; Lisowska, Halina; Banasik-Nowak, Anna; Arabski, Michał; Kedzierawski, Piotr; Florek, Agnieszka; Wojcik, Andrzej

    2010-01-01

    It is well known that cancer patients receiving similar radiotherapy treatments differ widely in normal tissue reactions ranging from undetectable to unacceptably severe levels. Therefore, an important goal of radiobiological research is to establish a test which would allow identifying individual radiosensitivity of patients prior to radiotherapy. The aim of the presented study is to assess the relationship between lymphocyte intrinsic radiosensitivity in vitro and early reaction of normal tissue in cervix cancer patients treated by radiotherapy. The following endpoints are analyzed in vitro: frequency of micronuclei, the kinetics of DNA repair and apoptosis. Acute normal tissue reaction to radiotherapy in the skin, bladder and rectum are scored according to the EORTC/RTOG scale. Our results show a wide inter-individual variability in chromosomal radiosensitivity in vitro. The majority of patients show a Grade 0, 1 or 2 reaction for all organs studied. No statistically significant correlation has been observed between the in vitro results in lymphocytes and the degree of early normal tissue and organ reaction.

  16. Dosimetric comparison between conventional and conformal radiotherapy for carcinoma cervix: Are we treating the right volumes?

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    Jyotirup Goswami

    2013-01-01

    Full Text Available Background and Purpose: Conventional portals, based on bony anatomy, for external beam radiotherapy for cervical cancer have been repeatedly demonstrated as inadequate. Conversely, with image-based conformal radiotherapy, better target coverage may be offset by the greater toxicities and poorer compliance associated with treating larger volumes. This study was meant to dosimetrically compare conformal and conventional radiotherapy. Materials and Methods: Five patients of carcinoma cervix underwent planning CT scan with IV contrast and targets, and organs at risk (OAR were contoured. Two sets of plans-conventional and conformal were generated for each patient. Field sizes were recorded, and dose volume histograms of both sets of plans were generated and compared on the basis of target coverage and OAR sparing. Results: Target coverage was significantly improved with conformal plans though field sizes required were significantly larger. On the other hand, dose homogeneity was not significantly improved. Doses to the OARs (rectum, urinary bladder, and small bowel were not significantly different across the 2 arms. Conclusion: Three-dimensional conformal radiotherapy gives significantly better target coverage, which may translate into better local control and survival. On the other hand, it also requires significantly larger field sizes though doses to the OARs are not significantly increased.

  17. [Cancer of cervix in Chile. Too much vaccine amid a neglected Papanicolau].

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    Fica, Alberto

    2014-04-01

    The Chilean Ministry of Health announced the incorporation of a human papillomavirus (HPV) vaccine to prevent cervix uterine cancer (CUC) into the national immunization program during year 2014 This decision was adopted despite of two opposing documents and a significant decrease in cervical cancer associated mortality due to cytological cervical screening. The burden of disease attributed to CUC has declined in Chile and current cost-effectiveness studies should be reviewed considering this decreasing trend, the progressive decrease in coverage rates observed during the past years, the potential need for aditional doses and lower vaccine costs if vaccine is acquired through the PAHO revolving fund. Moreover, serious adverse events associated with these vaccines, which in some countries are more frequent than CUC associated mortality, have not been thoroughly evaluated and are probably underreported. The decision to incorporate the vaccine occurs in a context of progressive weakening of the national cervical screening program leading to a reduced population coverage. This situation jepeordizes the achievements already obtained and poses a challenge to vaccine introduction considering that not all the high-risk viral subtypes are included and thus the risk for CUC does not disappear making cervical screening a vital component of the program that needs to be maintained. This governmental resolution requires a more solid scientific foundation and should not be implemented without resolving current cervical screening shortcomings.

  18. Regulation of Inflammatory Pathways in Cancer and Infectious Disease of the Cervix

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    Anthonio Adefuye

    2012-01-01

    Full Text Available Cervical cancer is one of the leading gynaecological malignancies worldwide. It is an infectious disease of the cervix, associated with human papillomavirus infection (HPV, infection with bacterial agents such as Chlamydia trachomatis and Neisseria gonorrhoea as well as human immunodeficiency virus (HIV. Furthermore, it is an AIDS-defining disease with an accelerated mortality in HIV-infected women with cervical cancer. With the introduction of robust vaccination strategies against HPV in the developed world, it is anticipated that the incidence of cervical cancer will decrease in the coming years. However, vaccination has limited benefit for women already infected with high-risk HPV, and alternative therapeutic intervention strategies are needed for these women. Many pathological disorders, including cervical cancer, are characterised by the exacerbated activation and maintenance of inflammatory pathways which are considered to be regulated by infectious agents. In cervical cancer, hyperactivation of these inflammatory pathways and regulation of immune infiltrate into tissues can potentially play a role not only in tumorigenesis but also in HIV infection. In this paper we will discuss the contribution of inflammatory pathways to cervical cancer progression and HIV infection and the role of HIV in cervical cancer progression.

  19. Identification of differentially expressed radiation-induced genes in cervix carcinoma cells using suppression subtractive hybridization

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    Kim, Jun Sang; Lee, Young Sook; Lee, Jeung Hoon; Lee, Woong Hee; Seo, Eun Young; Cho, Moon June [Chungnam National University, Daejeon (Korea, Republic of)

    2005-03-15

    A number of genes and their products are induced early or late following exposure of cells to ionizing radiation. These radiation-induced genes have various effects of irradiated cells and tissues. Suppression subtractive hybridization (SSH) based on PCR was used to identify the differentially expressed genes by radiation in cervix carcinoma cells. Total RNA and poly (A){sup +} mRNA were isolated from irradiated and non-irradiated HeLa cells. Forward-and reverse-subtracted cDNA libraries were constructed using SSH. Eighty-eight clones of each were used to randomly select differentially expressed genes using reverse Northern blotting (dot blot analysis). Northern blotting was used to verify the screened genes. Of the 176 clones, 10 genes in the forward-subtracted library and 9 genes in the reverse-subtracted library were identified as differentially expressed radiation-induced genes by PCR-select differential screening. Three clones from the forward-subtracted library were confirmed by Northern blotting, and showed increased expression in a dose-dependent manner, including a telomerase catalytic subunit and sodium channel-like protein gene, and an ESTs (expressed sequence tags) gene. We identified differentially expressed radiation-induced genes with low-abundance genes with SSH, but further characterization of theses genes are necessary to clarify the biological functions of them.

  20. Radiotherapy and surgery in the management of stage IB and IIA carcinoma of the cervix

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    Rabin, S.; Browde, S.; Nissenbaum, M.; Koller, A.B.; De Moor, N.G.

    1984-03-10

    Ninety-two consecutive patients with stages IB and IIA carcinoma of the cervix were treated by one of two regimens: pre-operative intracavitary caesium-137 followed by a Wertheim hysterectomy (group 1) or staging laparotomy with biopsy of suspicious lymph nodes (group 2) (if there was no evidence of tumour on examination of a frozen section of the lymph nodes a Wertheim hysterectomy was performed, but if a tumour was present the abdomen was closed and a course of radical irradiation given). When there was evidence of extracervical spread on examination of the specimen after the Wertheim hysterectomy, postoperative irradiation was given. It was found that clinical staging had been incorrect in 38.5% of patients thought to have stage IB disease and in 71.4% of those thought to have stage IIA disease. The actuarial survival curves showed no significant statistical difference between group 1 and group 2. When examination of the operative specimen showed tumour invasion of the parametrium only, the disease was well controlled by postoperative irradiation (all 7 such patients survived). On the other hand, when there was evidence of tumour invasion of pelvic lymph nodes the 4-year survival rate fell to 31% (4 out of 13 patients), despite the use of postoperative irradiation.

  1. High-dose weekly fractionation radiotherapy in advanced cancer of the uterine cervix

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    Browde, S.; Nissenbaum, M.; De Moor, N.G. (University of the Witwatersrand, Johannesburg (South Africa))

    1984-07-07

    A trial comparing two different radiotherapy techniques and schedules is the treatment of 83 patients with advanced cancer of the uterine cervix (stage IIIB) employing external irradiation alone is described. The one technique, used routinely in this department, employed a conventional daily fractionation schedule while the other used a high-dose weekly fractionation regimen. The techniques are described. The aim of the trial was to compare the efficacy and morbidity of these two methods of treatment. Dose distribution curves in cross-section and midsagittal planes are shown and calculations or equivalent doses at various selected points using Ellis's nominal single-dose formula are tabulated. The 2-year survival figures were 33% for the daily fractionation technique and 22% for the weekly regimen. Serious late complication rates were 6% for the daily regimen and 22% for the weekly schedule. These differences are not statistically significant. Late complication rates in the weekly fractionation regimen appeared to be lower than figures quoted by other authors. Local control within the irradiated volume was better in the group treated by the daily fractionation method.

  2. Treatment and outcome in cancer cervix patients treated between 1979 and 1994: A single institutional experience

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    Shyamkishore Shrivastava

    2013-01-01

    Materials and Methods: This is a retrospective study of 6234 patients with carcinoma of the cervix treated with radical intent between 1979 and 1994. All the work-up, staging investigations, treatment details, radiation protocols, outcomes, and toxicities were noted, compiled, and analyzed. Results: With a mean age of 46 years (range: 18-90 years; median: 45 years, 669 (11% patients were in stage Ib, 284 (5% were in stage IIa, 1891 (30% were in stage IIb, 69 (1% were in stage IIIa, and 3321 (53% were in stage IIIb. With a median follow-up of 68 months (57-79 months for the entire group, there was no significant difference in the outcome of 953 patients with international federation of gynecology and obstetrics (FIGO Ib-IIa treated after radical surgery, pre-operative radiation therapy (pre-op RT + Sx or after radical radiation; their disease-free survival (DFS was 60-62% at 8 years. In our series of 1891 patients with stage IIb and 3321 with stage IIIb, a respective DFS of 56% and 40% was achieved at 8 years. Conclusion: Over the last two decades, with the acquisition of newer facilities and inception of Joint Clinics, there has been a significant refinement in the treatment protocols and outcome. Improving radiation strategies to improve therapeutic ratio is the key to success.

  3. Ex vivo Mueller polarimetric imaging of the uterine cervix: a first statistical evaluation

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    Rehbinder, Jean; Haddad, Huda; Deby, Stanislas; Teig, Benjamin; Nazac, André; Novikova, Tatiana; Pierangelo, Angelo; Moreau, François

    2016-07-01

    Early detection through screening plays a major role in reducing the impact of cervical cancer on patients. When detected before the invasive stage, precancerous lesions can be eliminated with very limited surgery. Polarimetric imaging is a potential alternative to the standard screening methods currently used. In a previous proof-of-concept study, significant contrasts have been found in polarimetric images acquired for healthy and precancerous regions of excised cervical tissue. To quantify the ability of the technique to differentiate between healthy and precancerous tissue, polarimetric images of seventeen cervical conization specimens (cone-shaped or cylindrical wedges from the uterine cervix) are compared with results from histopathological diagnoses, which is considered to be the "gold standard." The sensitivity and specificity of the technique are calculated for images acquired at wavelengths of 450, 550, and 600 nm, aiming to differentiate between high-grade cervical intraepithelial neoplasia (CIN 2-3) and healthy squamous epithelium. To do so, a sliding threshold for the scalar retardance parameter was used for the sample zones, as labeled after histological diagnosis. An optimized value of ˜83% is achieved for both sensitivity and specificity for images acquired at 450 nm and for a threshold scalar retardance value of 10.6 deg. This study paves the way for an application of polarimetry in the clinic.

  4. Pemphigus vulgaris of the cervix: diagnostic difficulties associated with the Pap test.

    Science.gov (United States)

    Munhoz de Paula Alves Coelho, Karina; Stall, Jaqueline; Henrique Condeixa de França, Paulo; Cristina de Carvalho Tavares, Lara; Stefanello Bublitz, Giuliano; Loos, Beliza; Carvalho Costa, Luciana; Fronza Júnior, Hercílio

    2015-08-01

    Pemphigus vulgaris (PV) is a rare mucocutaneous disease caused by the abnormal production of antibodies against epithelial cell surface glycoproteins, resulting in loss of cell adhesion and intraepithelial blister formation. Cervical involvement in PV has been poorly reported, and there is little information regarding the criteria about consequential cytological changes identified in a Papanicolaou-stained cervicovaginal smear (Pap smear). Here, we report a case of PV manifesting in the cervix as well as the difficulty associated with the cytomorphological identification and interpretation of acantholytic cells. This case involved a 40-year-old patient with no history of Pap test abnormalities and no prior diagnosis of PV. In the cytological assessment, cells were identified both in isolation and in clusters that exhibited round nuclei of increased volume, inconspicuous nucleoli, and perinuclear halos. The patient underwent a cervical biopsy that revealed vesiculobullous lesions and morphological pattern consistent with PV. A skin biopsy confirmed this diagnosis. We concluded that knowledge of PV cytomorphology is important because difficulties associated with the identification and interpretation of acantholytic cells might be responsible for false positive diagnoses of cervical neoplasia. However, a suspected diagnosis of PV is possible if the cytological findings are carefully correlated with the clinical data.

  5. 放置IUD妇女阴道微生态状况调查分析%Investigation on the microecological status of the vagina IUD insertion

    Institute of Scientific and Technical Information of China (English)

    严韶; 苏洁; 江雯; 黄丽光; 候春雷; 张玲华

    2011-01-01

    目的 了解深圳市放置IUD妇女阴道微生态状况与年龄、居住类型、孕产史和个人生活习惯的关系.方法 对前来中心就诊要求放置IUD 609名已婚育龄妇女采用问卷调查、病史询问、妇科检查及阴道微生态检查,将被检者进行分类比较.结果 ①阴道微生态失调较高占26.1%(159/609),经常规检查进行上环术的受术者,尚有22.2%(124/558)阴道微生态失调;②随着年龄的增长,阴道微生态失调率随之增高,P<0.05;③与受教育程度相比,小学及以下文化程度的妇女诊断出微生态失调的可能性更高(42.8%);④微生态失调与阴道分娩次数也成呈正相关关系;⑤与非经期使用卫生护垫和阴道冲洗(间隔大于一周)等个人生活习惯,P=0.111,差异无统计学意义.结论 已婚育龄妇女阴道微生态变化受年龄、教育程度及分娩方式的影响.%Objective To investigate microecological status of the vagina of women who volunteer to receive IUD insertion, and to study the influence of age, residence type, maternal history and individual life habits on vaginal microecology. Methods 609 married women of reproductive age who volunteer to receive IUD insertion in our center were comparatively analyzed through questionnaire, medical history, gynecological and vaginal microecology examination. Results The rate of the vaginal microecology disorders is quite high, accounting for 26.1%. For women received IUD insertion, the rate was 22.2%. The rate of vaginal microecology disorders increased as the growth of age, with P<0.05. Women with no more primary school degree had higher risk of microecology disorders (42.8%). The ecological disorders and numbers of vaginal delivery are positively correlated. No correlation was found between vaginal microecology and individual life habits such as using health pads without menstrual period and vagina washing (more than a week) (P=0.111). Conclusion The conditions of vaginal

  6. Detection of hydrogen peroxide-producing Lactobacillus species in the vagina: a comparison of culture and quantitative PCR among HIV-1 seropositive women

    Directory of Open Access Journals (Sweden)

    Balkus Jennifer E

    2012-08-01

    Full Text Available Abstract Background The presence of hydrogen peroxide (H2O2 producing Lactobacillus in the vagina may play a role in controlling genital HIV-1 shedding. Sensitive molecular methods improve our ability to characterize the vaginal microbiota; however, they cannot characterize phenotype. We assessed the concordance of H2O2-producing Lactobacillus detected by culture with quantitative PCR (qPCR detection of Lactobacillus species commonly assumed to be H2O2-producers. Methods Samples were collected as part of a prospective cohort study of HIV-1 seropositive US women. Cervicovaginal lavage specimens were tested for L. crispatus and L. jensenii using 16S rRNA gene qPCR assays. Vaginal swabs were cultured for Lactobacillus and tested for H2O2-production. We calculated a kappa statistic to assess concordance between culture and qPCR. Results Culture and qPCR results were available for 376 visits from 57 women. Lactobacilli were detected by culture at 308 (82% visits, of which 233 of 308 (76% produced H2O2. L. crispatus and/or L. jensenii were detected at 215 (57% visits. Concordance between detection of L. crispatus and/or L. jensenii by qPCR and H2O2-producing Lactobacillus by culture was 75% (kappa = 0.45. Conclusions Among HIV-1 seropositive women, there was a moderate level of concordance between H2O2-producing Lactobacillus detected by culture and the presence of L. crispatus and/or L. jensenii by qPCR. However, one-quarter of samples with growth of H2O2-producing lactobacilli did not have L. crispatus or L. jensenii detected by qPCR. This discordance may be due to the presence of other H2O2-producing Lactobacillus species.

  7. The clinical significance of screening tests for precancerous lesions of the uterine cervix by means of liquid based cytology in female migrant workers%液基细胞学用于外来工妇女宫颈癌前病变筛查的临床意义

    Institute of Scientific and Technical Information of China (English)

    卢晓艳; 谢家滨; 杨步琴

    2011-01-01

    目的:探讨液基细胞学检查用于外来工妇女宫颈癌前病变筛查中的诊断价值及宫颈癌筛查现状.方法:应用液基细胞学(TCT)对到我院门诊就诊的外来工妇女(外来组)2 500名和进行常规宫颈癌前病变筛查的常住妇女1 600名(本地组)进行对比,并对其中细胞学筛查异常者进行阴道镜及镜下宫颈活检病理检查.结果:外来工妇女中,检测前有阴道排液或性生活出血等症状者878例,占35.12%,本地组有症状者87例,占5.43%.外来组共检测出细胞阳性368例,占14.72%,本地组异常例数为110例,占6.87%,两组比较,差异有统计学意义(P<0.05).对细胞学检查异常者进行阴道镜及镜下宫颈活检病理检查,其中CINⅠ级以上360例,符合率达75.3%.结论:门诊就诊的外来妇女与本地妇女相比较,宫颈癌前病变发病率较高,有症状者来院就诊比率明显升高,外来组妇女对宫颈癌前病变筛查认识普及率低下,因此常规对外来工就诊妇女进行液基细胞学检查,并结合阴道镜宫颈活检,是筛查和诊断宫颈癌前病变的可靠手段.%Objective: To discuss the diagnostic value of screening tests for precancerous lesions of the uterine cervix by means of liquid based cytology in female migrant workers and to survey the status of screening tests for precancerous lesions of the uterine cervix. Methods: 2 500 female migrant workers who went to our hospital for treatment in the method of liquid based cytology (TCT) compared with 1 600 towns women who went to our hospital for routine examination in screening tests for precancerous lesions of the uterine cervix, then examined the women whose index were abnormal by means of colposcopy and cervical biopsy. Results: 878 female migrant workers had the symptoms of draining the liquid from vagina before examination and bleeding during intercourse, accounting for 35.12%; and 87 towns women had the symptoms, accounting for 5.43%. There were 368 cases

  8. Primary primitive neuroectodermal tumor of the cervix confirmed with molecular analysis in a 23-year-old woman: A case report.

    Science.gov (United States)

    Masoura, Sophia; Kourtis, Anargyros; Kalogiannidis, Ioannis; Kotoula, Vassiliki; Anagnostou, Eleftherios; Angelidou, Stamatia; Agorastos, Theodoros

    2012-04-15

    Primitive Neuroectodermal Tumor (PNET) of the genital tract is very rare, especially in the cervix. A case report of a young woman with a diagnosis of PNET originating from the uterine cervix is presented here. A 23-year-old woman presented with abnormal uterine bleeding and sharp lower abdominal pain of two months duration. CT and MRI of the abdomen and thorax revealed the presence of a large mass in the uterine cervix, enlarged pelvic lymph nodes and broncho-pulmonary infiltrations with regional nodes. Pathological examination of the tumor revealed a malignant neoplasm composed predominantly of small cells, immunohistochemically positive for CD99, vimentin and c-Kit. Molecular testing demonstrated the expression of EWS/FLI1 fusion transcripts corresponding to the t(11;22)(q24;q12) translocation, which confirmed the diagnosis of PNET of the uterine cervix. Despite surgical excision and administration of the first cycle of adjuvant chemotherapy, the patient died from multiple-organ failure and cardiac arrest. PNET arising from the genital tract, especially in the uterine cervix, is very rare and presents a diagnostic challenge. A timely confirmation with molecular analysis is essential for the diagnosis of such a tumor at an unusual site as in the present case.

  9. [Primary diffuse large B-cell lymphoma of the uterine cervix successfully treated with rituximabplus cyclophosphamide, doxorubicin, vincristine, and prednisone chemotherapy-a case report].

    Science.gov (United States)

    Hashimoto, Akari; Fujimi, Akihito; Kanisawa, Yuji; Matsuno, Teppei; Okuda, Toshinori; Minami, Shinya; Doi, Tadashi; Ishikawa, Kazuma; Uemura, Naoki; Jyomen, Yuko; Tomaru, Utano

    2013-12-01

    Primary malignant lymphoma of the uterine cervix is a rare disease, and the therapeutic strategy has not been clearly established. A 45-year old woman presented with vaginal bleeding and hypermenorrhea in January 2012. Physical examination revealed a mass in the pelvic cavity approximately the size of a neonate's head. Pelvic magnetic resonance imaging(MRI) showed a solid mass 11 cm in size in the uterine cervix with homogeneous low intensity on T1-weighted images, iso-high intensity on T2-weighted images, and heterogeneous iso-high intensity on gadolinium-diethylenetriaminepentaacetate(Gd- DTPA)-enhanced images. Multiple lymphadenopathy were also detected in the pelvis. The Papanicolaou smear indicated class 5 cervical cytology, and a subsequent histological examination by a punch biopsy of the cervix showed diffuse infiltration of medium- to large-sized mononuclear cells that stained positive for CD20 and CD79a and negative for CD3, CD5, and EBER. Bone marrow biopsy revealed no abnormality. Positron emission tomography-computed tomography(PET-CT)showed strong fluorodeoxyglucose(FDG)accumulation in the uterine cervix mass, and in the pelvic and right inguinal lymphadenopathy. The patient was diagnosed with diffuse large B-cell lymphoma of the uterine cervix, Ann Arbor stage II AE. She was successfully treated with 8 courses of rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone(R-CHOP) chemotherapy, and maintains a complete remission.

  10. Copy number variation in glutathione-S-transferase T1 and M1 predicts incidence and 5-year survival from prostate and bladder cancer, and incidence of corpus uteri cancer in the general population

    DEFF Research Database (Denmark)

    Nørskov, M S; Frikke-Schmidt, R; Bojesen, S E;

    2011-01-01

    and GSTM1 were measured by real-time PCR in 10¿247 individuals, of whom 2090 had cancer. In men, the cumulative incidence of prostate cancer increased and the cumulative 5-year survival decreased with decreasing GSTT1 copy numbers (trends=0.02). The hazard ratios (HRs) (95% CIs) for prostate cancer...... and for death after prostate cancer diagnosis were, respectively, 1.2 (0.8-1.8) and 1.2 (0.6-2.1) for GSTT1*1/0, and 1.8 (1.1-3.0) and 2.2 (1.1-4.4) for GSTT1*0/0 versus GSTT1*1/1. In women, the cumulative incidence of corpus uteri cancer increased with decreasing GSTT1 copy numbers (trend=0.04). The HRs...... were, respectively, 1.5 (0.7-3.2) and 2.0 (0.9-4.3) for GSTM1*1/0 and GSTM1*0/0 versus GSTM1*1/1. The HR for death after bladder cancer diagnosis was 1.9 (1.0-3.7) for GSTM1*0/0 versus GSTM1*1/0. In conclusion, exact CNV in GSTT1 and GSTM1 predict incidence and 5-year survival from prostate and bladder...

  11. Vagina: What's Normal, What's Not

    Science.gov (United States)

    ... 2015. Robinson JN. Approach to episiotomy. http://www.uptodate.com/home. Accessed Feb. 4, 2015. Fashokun TB, ... organ prolapse in women: Diagnostic evaluation. http://www.uptodate.com/home. Accessed Feb. 5, 2015. Karam A, ...

  12. Comparative study vagina mould/ fletcher applicator in MRI guided pulsed rate brachytherapy in patients reached by an uterine cervix carcinoma; Etude comparative moule vaginal/applicateur de fletcher en curietherapie de debit pulse guide par IRM chez les patientes atteintes d'un cancer du col uterin

    Energy Technology Data Exchange (ETDEWEB)

    Francois, G.; Dumas, I.; Mazeron, R.; Verezesan, O.; Monnier, L.; Vieillot, S.; Gensse, M.C.; Haie-Meder, C. [Institut Gustave-Roussy, 94 - Villejuif (France)

    2009-10-15

    This study began in february 2009 and included until today ten patients. twenty patients are planned. The analysis of preliminary results show a much higher tolerance for the vaginal cast device. The dosimetry data were the object of a preliminary study that seems demonstrate an equivalence of the both modalities, with differences concerning the doses to critical organs; The definitive result swill be presented with the data including the twenty planned patients. (N.C.)

  13. Stating of cervical carcinoma using magnetic resonance imaging; Estadificacion del carcinoma de cervix por resonancia magnetica

    Energy Technology Data Exchange (ETDEWEB)

    Oleaga, L.; Vela, M. C.; Grande, J.; Cura del, J. L.; Grande, D. [Hospital de Basurto. Bilbao (Spain)

    1999-07-01

    The infiltration of the parametrium represents one of the most important factors that determine the prediction and treatment of cervical carcinoma. Our objetive is to evaluate the utility of magnetic resonance imaging (MRI) in the staging of cervical carcinomas, to establish the reliability of this technique and to carry out a comparative study of the sequences used to demonstrate the parametrial invasion. We have carried out a retrospective study on 44 patients diagnosed with cervix neoplasia, using clinical exploration and performing a biopsy. the MRI studies have been carried out using a 1 Tesla magnet and the sequences used have been SE T1, Se proton density (PD) and T2 and dynamic GRE after administering gadolinium intravenously in the axial and sagital projections. The stages determined by MRI have been compared to the anatomopathological stages of the surgical specimens in cases where surgery was carried out and with the clinical stage in cases where no radical surgery was carried out. A diagnosis value of MRI has been determined to demonstrate the parametrial invasion, comparing the SE T2 sequence with the dynamic GE sequence with gadolinium. We calculate the volume of the tumour in the MRI studies to evaluate the difference of the volume between patients with tumoral stages that are clinically surgical and not surgical. MRI determines the invasion of the parametrium with a sensitivity of 88.8%, a specificity of 80% a positive value of 76.1%, a negative predictive value of 90.9% and a reliability of 83.7%. For the SE T2 sequences the sensitivity was 86.6%, the specifity 80%, the posistive predictive value 81.25%, the negative predictive value 85.7% and the reliability 83.3%. For the dynamic GE sequence with intravenous gadolinium the sensitivity was 86.6%, the specifity 86.6%, the posistive predictive value 86.6%, the negative predictive value 86.6% and the reliability 86.6%. The use of the dynamic GE sequence after the intravenous administration of

  14. Coriocarcinoma primário do colo uterino Primary choriocarcinoma of the uterine cervix

    Directory of Open Access Journals (Sweden)

    Maria Fernanda Moreira Ferraz

    2003-06-01

    Full Text Available Coriocarcinomas geralmente ocorrem no corpo uterino durante o período reprodutivo. Raramente podem acontecer alterações de localização e de idade de acometimento. Coriocarcinomas primários do colo uterino são extremamente raros, geralmente ocorrem no pós-parto de seis meses a dois anos e se apresentam com sangramento por via vaginal. Existem três teorias para o desenvolvimento dos coriocarcinomas no colo uterino: 1 a paciente ter tido uma gestação cervical que sofreu transformação maligna; 2 que o coriocarcinoma da cérvice seja uma metástase de um tumor primário do corpo que desapareceu; 3 que seu desenvolvimento seja devido ao transporte de células coriônicas da gestação precedente como êmbolos, os quais ficaram latentes e posteriormente sofreram transformação maligna. A terapêutica preconizada é a realização de histerectomia com manutenção dos anexos e posterior quimioterapia. Relatamos o caso de uma mulher de 34 anos que, seis meses após parto normal, iniciou com sangramento vaginal. O exame especular mostrou massa vegetante e hemorrágica do colo uterino e a dosagem de gonadotrofina coriônica humana fração b (b-HCG revelou altos níveis sangüíneos. O exame histopatológico mostrou uma neoplasia maligna composta por sincício e citotrofoblasto malignos comprometendo apenas o colo uterino. Nosso caso é um coriocarcinoma ectópico e acreditamos que seu desenvolvimento no colo uterino seja devido ao transporte de células coriônicas da gravidez precedente que sofreram posterior transformação maligna.Choriocarcinomas usually occur within the body of the uterus during reproductive years. On rare occasions they may occur abnormally in relation to place and time. Primary choriocarcinomas of the uterine cervix are extremely rare. They usually occur in a latent period of six months to two years after the preceding pregnancy, and present with disfunctional vaginal bleeding. Theoretically, there are three

  15. Topotecan for the treatment of recurrent and stage IVB carcinoma of the cervix.

    Science.gov (United States)

    Paton, F; Paulden, M; Saramago, P; Manca, A; Misso, K; Palmer, S; Eastwood, A

    2010-05-01

    This paper presents a summary of the evidence review group (ERG) report into the clinical effectiveness and cost-effectiveness of topotecan in combination with cisplatin for the treatment of recurrent and stage IVB carcinoma of the cervix, in accordance with the licensed indication, based upon the evidence submission from the manufacturer to the National Institute for Health and Clinical Excellence (NICE) as part of the single technology appraisal (STA) process. The outcomes measured were overall survival, progression-free survival, response rates, adverse effects of treatment, health-related quality of life (HRQoL) and quality-adjusted life-years (QALYs) gained. The manufacturer stated that topotecan plus cisplatin is the only combination regimen to date to have demonstrated a statistically significant survival advantage compared to cisplatin monotherapy in the licensed population. The clinical evidence came from three clinical trials comparing topotecan plus cisplatin with cisplatin monotherapy (GOG-0179), topotecan plus cisplatin with paclitaxel plus cisplatin (GOG-0169), and four cisplatin-based combination therapies: topotecan plus cisplatin, paclitaxel plus cisplatin, gemcitabine plus cisplatin, and vinorelbine plus cisplatin (GOG-0204). Results from GOG-0179 showed greater median overall survival with topotecan plus cisplatin than with cisplatin monotherapy: 9.4 months versus 6.5 months. Similar results were also reported for median progression-free survival. Response rates also showed an advantage with topotecan plus cisplatin compared with cisplatin monotherapy. The response rates in patients receiving cisplatin monotherapy were very low, but the potential reasons for this were not discussed in the manufacturer's submission. Patients receiving topotecan plus cisplatin experienced a greater number of adverse events and the ERG was concerned with some of the assumptions related to HRQoL. In the base-case direct comparison, the incremental cost

  16. Effect of Intravenous Dexamethasone on Preparing the Cervix and Labor Induction

    Directory of Open Access Journals (Sweden)

    Fatemeh Laloha

    2015-10-01

    Full Text Available The use of corticosteroids is one of the methods put forward for the strengthening and speeding up the process of labor. After identification of glucocorticoid receptors in human amnion, the role of corticosteroids in starting the process of labor has been studied in numerous studies. The purpose of this study was to determine the effect of intravenous Dexamethasone on preparing the cervix and on labor induction. A randomized, clinical, and double – blind trial was conducted on 172 women divided into a control and an experimental group. The inclusion criteria were that they had to be primparous, in or before the 40th week of pregnancy, and with Bishop scores (B.S.s of 4 or lower. The exclusion criteria were diabetes, preeclampsia, macrosomia, twin pregnancy, rupture of the membrane (ROM, breech, and women suffering from background diseases. The B.S.s of the women was measured in charge of the study, and each woman was intravenously injected with eight milligrams of Dexamethasone or eight milligrams of distilled water. Four hours after the injections, the B.S.s of the participants was measured, and they were put under the conditions of labor induction using oxytocin. Information was collected in checklists A and B. The patients were compared with respect to B.S., the time the induction started, the average interval between the start of induction and the beginning of the active phase of childbirth, and the average length of time between the start of the active phase and the second stage of childbirth. The first and five minutes Apgar scores of the two groups of women were compared. The frequencies, the means,  and the standard deviations were calculated using the SPSS – 16 software, and analysis of the results was performed with the Student’s t- test and the chi-square test with PPPP

  17. [Premature birth in patient with cervix incompetence and history of myasthenia gravis].

    Science.gov (United States)

    Fuentealba, Maximiliano; Troncoso, Miguel; Vallejos, Joaquin; Ponce, Sebastian; Villablanca, Nelson; Melita, Pablo

    2013-09-01

    Cervical incompetence it's a dilatation of the cervix during the third trimester of pregnancy that ends with the interruption of it. The incidence in Chile is about 0.1-2% of the total pregnancies and it's one of the causes of preterm birth. A 34 years old pregnant patient. Timectomized at age 18 to treat her miastenia gravis, previously trated with medication, had 4 previous preterm labours all of them under 25 weeks and vaginal births. All fetuses died postpartum. A cerclage was made during the third, fourth and fifth pregnancies. She didn't present hypertension during the gestation and no cervical diameter under 15mm. Since the fourth gestation the following tests are taken: Antifosfolipidic antibodies, APTT,PT. All the results are either normal or negative. Microbial cultures were negative. No amniocentesis was made. A McDonald cervical cerclage was made during pregnancies number 3, 4 and 5 on the 16th week to delay the labor. Also oral micronized progesterone, on a 400mg/24 hours dosis, was administered to avoid preterm birth. On the 24th week the pharmacological treatment started including Intramuscular Betamethasone, 12 mg/24 hours (2 doses), to induce lung maturity on the fetus. It is thought that the administration of progesterone could have improved the situation of the patient, because it acts as a labour repressants. The use of cerclage could have helped, but the factors that may influence the effectiveness of this method are unknown. Perhaps there is some immunologic factor associated with the miastenia gravis that alters the normal course of pregnancy.

  18. Cell-mediated infection of cervix derived epithelial cells with primary isolates of human immunodeficiency virus.

    Science.gov (United States)

    Tan, X; Phillips, D M

    1996-01-01

    We have previously demonstrated that HIV-infected transformed T-cells or monocytes adhere to monolayers of CD4-negative epithelial cells. Adhesion is soon followed by budding of HIV from infected mononuclear cells onto the surface of epithelial cells. Epithelial cells subsequently take up virus and become productively infected. Based on these findings, we proposed that sexual transmission of HIV may involve cell-mediated infection of intact mucosal epithelia of the urogenital tract. However, it has become increasingly clear that primary cells and HIV strains isolated from patients are more appropriate models for HIV infection than established cell lines and lab strains of virus. In the studies described here, we infected cervix-derived epithelial monolayers with primary monocytes infected with patient isolates of non-syncytial inducing (NSI) macrophage-tropic strains of HIV. Under the culture conditions employed, HIV-infected primary monocytes do not remain adherent to the apical surface of the epithelium, as did HIV-infected transformed cells. Instead, following adherence, the primary cells migrate between epithelial cells. Virus is secreted from a pseudopod as HIV-infected primary monocytes pass between cells of the epithelium. Productive infection of the epithelium was detected by p24 ELISA and PCR Southern blot analysis. Infection can be blocked by sera from HIV-seropositive individuals or by certain sulfated polysaccharides. These findings support the supposition that transmission of HIV may occur via cell-mediated infection of intact epithelia. The observations also hint at the possibility that-HIV-infected monocyte/macrophages in semen or cervical-vaginal secretions could cross intact epithelia by passing between epithelial cells. Blocking studies suggest that it may be possible to inhibit sexual transmission of HIV either by antibodies in genital tract secretions or by a topical formulation containing certain sulfated polysaccharides.

  19. Dual regulation of energy metabolism by p53 in human cervix and breast cancer cells.

    Science.gov (United States)

    Hernández-Reséndiz, Ileana; Román-Rosales, Alejandra; García-Villa, Enríque; López-Macay, Ambar; Pineda, Erika; Saavedra, Emma; Gallardo-Pérez, Juan Carlos; Alvarez-Ríos, Elizabeth; Gariglio, Patricio; Moreno-Sánchez, Rafael; Rodríguez-Enríquez, Sara

    2015-12-01

    The role of p53 as modulator of OxPhos and glycolysis was analyzed in HeLa-L (cells containing negligible p53 protein levels) and HeLa-H (p53-overexpressing) human cervix cancer cells under normoxia and hypoxia. In normoxia, functional p53, mitochondrial enzyme contents, mitochondrial electrical potential (ΔΨm) and OxPhos flux increased in HeLa-H vs. HeLa-L cells; whereas their glycolytic enzyme contents and glycolysis flux were unchanged. OxPhos provided more than 70% of the cellular ATP and proliferation was abolished by anti-mitochondrial drugs in HeLa-H cells. In hypoxia, both cell proliferations were suppressed, but HeLa-H cells exhibited a significant decrease in OxPhos protein contents, ΔΨm and OxPhos flux. Although glycolytic function was also diminished vs. HeLa-L cells in hypoxia, glycolysis provided more than 60% of cellular ATP in HeLa-H cells. The energy metabolism phenotype of HeLa-H cells was reverted to that of HeLa-L cells by incubating with pifithrin-α, a p53-inhibitor. In normoxia, the energy metabolism phenotype of breast cancer MCF-7 cells was similar to that of HeLa-H cells, whereas p53shRNAMCF-7 cells resembled the HeLa-L cell phenotype. In hypoxia, autophagy proteins and lysosomes contents increased 2-5 times in HeLa-H cells suggesting mitophagy activation. These results indicated that under normoxia p53 up-regulated OxPhos without affecting glycolysis, whereas under hypoxia, p53 down-regulated both OxPhos (severely) and glycolysis (weakly). These p53 effects appeared mediated by the formation of p53-HIF-1α complexes. Therefore, p53 exerts a dual and contrasting regulatory role on cancer energy metabolism, depending on the O₂level.

  20. Maturation of the uterine cervix by repeated intracervical instillation of prostaglandin E2.

    Science.gov (United States)

    Milliez, J M; Jannet, D; Touboul, C; el Medjadji, M; Paniel, B J

    1991-09-01

    An attempt was made to ripen the uterine cervix in 100 high-risk pregnancy patients (pregnancy between 34 to 41 weeks' gestation), with the use of intracervical instillations of 0.25 mg of prostaglandin E2 mixed with a tylose gel. The maturation process was repeated every 48 hours. Forty-nine patients were delivered of infants after the first maturation and 51 patients required between two and nine instillations. In patients requiring multiple instillations, the mean delay between the first procedure and delivery was 9 +/- 4 days (range, 2.4 to 16 days). Among the 59 nulliparous women, only 23 were delivered of infants after a single maturation and 36 required multiple maturations (p less than 0.02). When the group of patients who were delivered of infants after a single maturation process was compared with the group requiring multiple maturations, no difference could be seen with regard to age, term of pregnancy, or Bishop cervical score at the time of inclusion in the study. The myometrial activity and the onset labor induced by prostaglandin E2, were similar in both groups. Fetal heart rate decelerations occurred in 16.3% (8/49) of the patients with single maturations and in 17.6% (9/51) of the patients who required multiple maturations. The outcome of the pregnancy and the rate of cesarean sections (24% and 27%) were similar in both groups. No patients required cesarean sections because of failed induction of labor. Cervical ripening after repeated applications of 0.25 mg of prostaglandin E2 seems to be safe for the fetus, providing that the patient is closely supervised.

  1. Vagal afferents from the uterus and cervix provide direct connections to the brainstem.

    Science.gov (United States)

    Collins, J J; Lin, C E; Berthoud, H R; Papka, R E

    1999-01-01

    Previous anatomical studies demonstrated vagal innervation to the ovary and distal colon and suggested the vagus nerve has uterine inputs. Recent behavioral and physiological evidence indicated that the vagus nerves conduct sensory information from the uterus to the brainstem. The present study was undertaken to identify vagal sensory connections to the uterus. Retrograde tracers, Fluorogold and pseudorabies virus were injected into the uterus and cervix. DiI, an anterograde tracer, was injected into the nodose ganglia. Neurectomies involving the pelvic, hypogastric, ovarian and abdominal vagus nerves were performed, and then uterine whole-mounts examined for sensory nerves containing calcitonin gene-related peptide. Nodose ganglia and caudal brainstem sections were examined for the presence of estrogen receptor-containing neurons in "vagal locales." Labeling of uterine-related neurons in the nodose ganglia (Fluorogold and pseudorabies virus) and in the brainstem nuclei (pseudorabies virus) was obtained. DiI-labeled nerve fibers occurred near uterine horn and uterine cervical blood vessels, in the myometrium, and in paracervical ganglia. Rats with vagal, pelvic, hypogastric and ovarian neurectomies exhibited a marked decrease in calcitonin gene-related peptide-immunoreactive nerves in the uterus relative to rats with pelvic, hypogastric, and ovarian neurectomies with intact vagus nerves. Neurons in the nodose ganglia and nucleus tractus solitarius were immunoreactive for estrogen receptors. These results demonstrated: (1) the vagus nerves serve as connections between the uterus and CNS, (2) the nodose ganglia contain uterine-related vagal afferent neuron cell bodies, and (3) neurons in vagal locales contain estrogen receptors.

  2. Knowledge and Practices of Nurses Working in an Education Hospital on Early Diagnosis of Breast and Cervix Cancers.

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    Ozlem Ozdemir

    2010-12-01

    Full Text Available AIM: This research has aimed to determine knowledge and practice status of nurses about breast self-examination (BSE, clinical breast examination (CBE, mammography and Pap smear and about influencing status of some variables related to these examinations. METHOD: This descriptive study was conducted in an education hospital in Ankara between March 1st and May 30th, 2008. Three hundred-fifty nurses (82.7% have accepted to participate in the study. Data were collected by a questionnaire form including questions about demographics, their knowledge and practice status about BSE, CBE, mammography and Pap smear. Chi-square test, numbers and percentages were used for evaluating the data. RESULTS: Overall, 46.9% of nurses had enough knowledge about early diagnosis of breast and cervix cancer. 60.2% of them can carry BSE, 18.8% can carry out CBE and 7.3% can carry out mammography. Pap smear is carried out by 23.7% of the nurses. Negligence, fear of cancer and thought of finding them unnecessary were determined as reasons for avoidance. Their knowledge and practice were significantly different (p<0.05 according to their age and service where they work. CONCLUSION: It has been concluded that although knowledge and practices of nurses on breast and cervix cancer are at a good level, this isn’t enough when importance of early diagnosis in breast and cervix cancer are taken into consideration, which are among common cancers in women. [TAF Prev Med Bull 2010; 9(6.000: 605-612

  3. Carcinoma of uterine cervix with isolated metastasis to fibula and its unusual behavior: Report of a case and review of literature

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    Pasricha Rajesh

    2006-01-01

    Full Text Available Bone metastasis from carcinoma cervix is uncommon, especially in the distal appendicular skeleton. A 36 year old lady presented with carcinoma of uterine cervix, FIGO, stage IIb. She was treated with radical radiotherapy. Nine months later, she developed an isolated lytic lesion in right fibula, which turned out to be a metastatic lesion. The patient is doing well, 3 years after the surgical excision of metastasis. This is one of the few documented cases of metastasis to fibula, arising from carcinoma of uterine cervix and probably the first with isolated metastasis of this site. Unlike the dismal outcome commonly seen in patients with bone secondaries, she continues to be disease free and alive at 39 months of follow up, after the development of skeletal metastasis.

  4. Spontaneous rupture of bladder diverticulum after postoperative radiotherapy for carcinoma of the uterine cervix. A case report

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    Nishimura, Tetsuo; Suzuki, Kazunori; Iijima, Mitsuharu; Nozue, Masashi; Imai, Michiko; Suzuki, Sachiko; Sakahara, Harumi; Ohta, Nobutaka; Kasami, Masako [Hamamatsu Univ. School of Medicine, Shizuoka (Japan)

    2000-08-01

    We present a case of spontaneous rupture of bladder diverticulum three years after postoperative whole pelvic irradiation (50.4 Gy) for carcinoma of the uterine cervix. The patient had suffered from a neurogenic bladder after hysterectomy, but excretory urography revealed no abnormalities. Bladder diverticulum was found two years later. Spontaneous rupture of the urinary bladder is one of the late complications associated with radiotherapy, although it is very rare. Postoperative neurogenic bladder may also be associated with rupture. We should be aware of this rare complication in patients who receive pelvic irradiation. (author)

  5. A study of the prognostic role of serum fucose and fucosyl transferase in cancer of the uterine cervix.

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    Sen,Urmi

    1985-04-01

    Full Text Available Serum fucose levels and fucosyl transferase activities have been designated as nonspecific markers of malignancy, and play an important role in the diagnosis of different types of malignancies. In the present study, attempts were made to determine the prognostic significance of these markers in patients with cancer of the uterine cervix after therapy. It was found that both serum fucose and fucosyl transferase, which were elevated in untreated patients declined significantly in patients responsive to therapy at different follow-up intervals, but not in patients unresponsive to therapy.

  6. Citologia Hormonal do Trato Urinário Baixo e da Vagina de Mulheres na Pós-menopausa, antes e durante Estrogenioterapia Oral e Transdérmica

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    Lustosa Áurea Belas

    2002-01-01

    Full Text Available Objetivo: avaliar a ação dos estrogênios por via oral ou transdérmica nas células do trato urinário baixo e da vagina, em mulheres menopausadas. Métodos: foram incluídas 25 mulheres na pós-menopausa, nas quais se estudaram os efeitos citológicos da terapia de reposição hormonal estrogênica, por via oral e por via transdérmica, sobre as células da vagina e do sedimento urinário. As pacientes foram distribuídas aleatoriamente em 2 grupos: Grupo I, constituído por 14 mulheres que receberam 0,625 mg de estrogênio conjugado eqüino associado a 5 mg de acetato de medroxiprogesterona, por via oral, diariamente sem intervalo por três meses, e Grupo II, formado por 11 mulheres que fizeram uso de 17-beta-estradiol matricial na dose de 50 mig, por via transdérmica, semanalmente, associado a 5 mg de acetato de medroxiprogesterona diariamente por três meses. As amostras urinárias foram obtidas do jato inicial da primeira urina da manhã, após asseio dos genitais, em frascos estéreis fornecidos pelo laboratório. A urina foi centrifugada e o esfregaço realizado a partir do sedimento urinário. Os esfregaços da vagina e do sedimento urinário foram imediatamente fixados em álcool absoluto e corados pelo método de Shorr. Resultados: observou-se que nas pacientes que utilizaram a via oral, houve maturação das células da vagina (o índice se elevou de 45,4 a 65,5 com dois meses de tratamento, permanecendo praticamente constante (62,0 a seguir. Quanto às células urinárias, não houve variação do valor de maturação (56,4 antes da medicação e 60,4 no final do período. Ao examinarmos tanto as células da urina quanto da vagina de pacientes que utilizaram a via transdérmica, notou-se que houve resposta satisfatória. Conclusão: com estes resultados podemos sugerir que os estrogênios, quando administrados por via transdérmica, estão associados à resposta satisfatória quanto ao trofismo tanto na vagina quanto na uretra

  7. [An ultrastructural study of the cervix epitelium infected with the human papillomavirus types 16 and 18 before and after treatment with contrasting thermo-laser therapy].

    Science.gov (United States)

    Manykin, A A; Ezhov, V V; Belov, S V; Danileĭko, Iu K; Saliuk, V A; Dymkovets, V P; Gushchina, E A; Lisitsyn, F V

    2014-01-01

    The results of the ultrastructural study of the epithelium of the patient cervix infected by the human papillomavirus (HPV) types 16 and 18 before and after treatment by contrasting thermo-laser therapy (CTLT) are presented. It was shown in this work that 1.5 and 6 months after treatment HPV DNA was not detected in the biopsy and the smear of the cervix using the polymerase chain reaction (PCR). In the ultrathin sections, the structure of the epithelial cells from the biopsy after treatment corresponded to norm. There was effective elimination of HPV types 16 and 18 as Induces by CTLT method.

  8. Reference data representative of normal findings at two-dimensional and three-dimensional gray-scale ultrasound examination of the cervix from 17 to 41 weeks' gestation.

    OpenAIRE

    Rovas, Linas; Sladkevicius, Povilas; Strobel, E; Valentin, Lil

    2006-01-01

    Objectives To create reference values representative of normal findings on two-dimensional (2D) and three-dimensional (3D) transvaginal ultrasound (TVS) examination of the cervix from 17 to 41 weeks' gestation and to determine the agreement between cervical measurements taken by 2D and 3D TVS. Methods Cross-sectional study covering 17 to 41 weeks in 419 nulliparous and 360 parous women who delivered at term and who underwent 2D and 3D TVS examination of the uterine cervix. We examined app...

  9. 复发性外阴阴道假丝酵母菌病发病机制的研究进展%An analysis of the factors contributing to recurrent vulva and vagina candidiasis

    Institute of Scientific and Technical Information of China (English)

    徐倩倩; 宁玉梅

    2013-01-01

    复发性外阴阴道假丝酵母菌病(Recurrent candidiasis of vulva and vagina,RVVC)病因复杂,主要原因有机体免疫防御机制的变化,宿主局部因素,假丝酵母菌的菌群多样性变化等.认识、了解及掌握上述因素,对临床工作者有效防治RVVC起着非常重要作用.

  10. Conventional four field radiotherapy versus computed tomography-based treatment planning in cancer cervix: A dosimetric study

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    Abhishek Gulia

    2013-01-01

    Full Text Available Background: With advancements in imaging, wide variations in pelvic anatomy have been observed, thus raising doubts about adequate target volume coverage by conventional external radiotherapy fields based on bony landmarks. The present study evaluates the need for integrating computed tomography (CT-based planning in the treatment of carcinoma cervix. Aims: To estimate inadequacies in target volume coverage when using conventional planning based on bony landmarks. Materials and Methods: The study consisted of 50 patients. Target volume delineation was done on planning CT scans, according to the guidelines given in literature. The volume of target receiving 95% of prescribed dose (V95 was calculated after superimposing a conventional four field box on digitally reconstructed radiograph. The geographic miss with conventional four field box technique was compared with the CT-based target volume delineation. Results: In 48 out of 50 patients, the conventional four field box failed to encompass the target volume. The areas of miss were at the superior and lateral borders of the anterior-posterior fields, and the anterior border of the lateral fields. The median V95 for conventional fields marked with bony landmarks was only 89.4% as compared to 93% for target delineation based on CT contouring. Conclusions: Our study shows inadequate target volume coverage with conventional four field box technique. We recommend routine use of CT-based planning for treatment with radiotherapy in carcinoma cervix.

  11. Dosimetric study comparing intensity modulated and conformal pelvic radiotherapy boost plans in locally advanced cancer cervix in NCI-Cairo

    Institute of Scientific and Technical Information of China (English)

    Mohamed Mahmoud; Hesham A. EL-Hossiny; Nashaat A. Diab; Mahmoud Shosha

    2013-01-01

    Objective: This study was to compare 5 field conformal technique to the intensity modulated radiotherapy (IMRT) 8 fields technique in boosting locally advanced cancer cervix cases after external beam radiotherapy with respect to target volume coverage and dose to normal tissues. Methods: We conducted a single institutional comparative dosimetric analysis of 10 patients with cancer cervix who was presented to radiotherapy department in National Cancer Institute, Cairo in period between June 2012 to September 2012 and received a CRT boost in the place of planned brachytherapy after large field pelvic radiotherapy (PRT) with concurrent chemotherapy were retrospectively identified. All tumors were situated in the low central pelvis. Two plans were done for every patient; one using the 8 fields IMRT and the second one using 5 fields' 3DCRT the two techniques were then compared using dose volume histogram (DVH) analysis for the PTV, bladder, rectum and both femoral heads. Results: Comparing different DVHs, it was found that the planning target volume (PTV) was adequately covered in both plans while it was demonstrates that the 8 fields IMRT technique carried less doses reaching OARs (rectum, bladder, both femoral heads). Conclusion: From the present study, it is concluded that IMRT technique spared more efficiently OARs than CRT technique but both techniques covered the PTV adequately so whenever possible IMRT technique should be used.

  12. Adenocarcinoma involving the uterine cervix: magnetic resonance imaging findings in tumours of endometrial, compared with cervical, origin

    Energy Technology Data Exchange (ETDEWEB)

    Haider, M.A. [Univ. of Toronto, Dept. of Medical Imaging, Univ. Health Network, Mount Sinai Hospital, Toronto, Ontario (Canada)]. E-mail: mhaider@utoronto.ca; Patlas, M. [McMaster Univ., Dept. of Radiology, Hamilton General Hospital, Hamilton, Ontario (Canada); Jhaveri, K. [Univ. of Toronto, Dept. of Medical Imaging, Univ. Health Network, Mount Sinai Hospital, Toronto, Ontario (Canada); Chapman, W. [Univ. of Toronto, Dept. of Pathology, Univ. of Health Network, Toronto, Ontario (Canada); Fyles, A. [Univ. of Toronto, Dept. of Radiation Oncology, Princess Margaret Hospital, Univ. Health Network, Toronto, Ontario (Canada); Rosen, B. [Univ. of Toronto, Dept. of Obstetrics and Gynecology, Univ. Health Network, Toronto, Ontario (Canada)

    2006-02-15

    To determine the distinctive magnetic resonance imaging (MRI) features of cervical and endometrial adenocarcinoma that present clinically as cervical mass. From 1999 to 2002, 56 patients with adenocarcinoma on the initial biopsy of a cervical mass underwent MRI at our institution. Of these, 42 had a visible mass on MRI. Pathology review of all available tissue was the reference standard. A site of origin was determined by the pathologist in 38 of the 42 patients, and these were the cases evaluated; of these patients, 32 cases had adenocarcinoma and 6 had adenosquamous cancers. Findings were significantly more prevalent in patients with adenocarcinomas of endometrial, compared with cervical, origin for endometrial thickening (11 [73%] and 3 [13%], respectively; P = 0.0003), endometrial mass (11 [73%] and 1 [4%], respectively; P < 0.0001), endometrial cavity expansion by a mass (9 [60%] and 2 [9%], respectively; P = 0.001), and invasion of myometrium from endometrium (9 [60%] and 0, respectively; P < 0.0001). Adenocarcinomas of the endometrium that involve the cervix have MRI features that help distinguish them from primary adenocarcinomas of the cervix. (author)

  13. Prostaglandin treatment is associated with a withdrawal of progesterone and androgen at the receptor level in the uterine cervix

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    Ekman-Ordeberg Gunvor

    2009-10-01

    Full Text Available Abstract Treatment with prostaglandin(PG-E2 is clinically efficient for cervical priming. The aim of this study was to evaluate the impact of PG-E2 on the expression of the progesterone (PR, androgen (AR and glucocorticoid (GR receptors in human uterine cervix in prolonged pregnancy. The study groups were postterm nulliparous women with unripe cervices undergoing cervical priming with PG-E2 before labor induction. Responders (n = 12 who delivered vaginally were compared with non-responders (n = 10, who underwent cesarean section due to failure to progress to the active phase of labor. Controls (n = 18 with vaginal partus at a normal gestational age served as a reference group. Cervical levels of PR-A and PR- B isoforms, AR and GR, serum levels of their ligands and sex hormone-binding globulin (SHBG were quantified. The responder group displayed lower total PR-AB and AR protein levels as compared to non-responders, and lower PR-B and AR protein levels as compared to controls. In addition, the PR mRNA level was lower in responders as compared to non-responders. The GR protein level did not differ between the groups. We conclude that successful PG-E2 priming was followed by a progesterone and androgen withdrawal at the receptor level in the uterine cervix.

  14. Effect of NBS1 gene polymorphism on the risk of cervix carcinoma in a northern Indian population.

    Science.gov (United States)

    Sobti, R C; Shekari, M; Kordi Tamandani, D M; Kaur, P; Suri, V; Huria, A

    2008-01-01

    Cervical cancer is one of the most common neoplastic diseases affecting women, with a worldwide incidence of almost half a million cases. A history of smoking and use of oral contraceptives have been confirmed to be risk factors for cervical cancer. Genetic susceptibility and immune response, especially impaired cellular immune response, may well be related to the development of cervical cancer. NBS1 is one of the key proteins participating in the recognition and repair of double-strand breaks that may lead to genomic instability and cancer if unrepaired. The objective of the present study was therefore to investigate NBS1 Glu185Gln gene polymorphisms and the risk of cervix cancer in a northern Indian population. We found that passive smokers having particular NBS1 genotypes (Glu/Gln, Gln/Gln or Glu/Gln + Gln/Gln)have an increased risk of developing cervix cancer (OR 5.21, p=0.000001; OR 4.60, p=0.001; OR 5.10, p=0.0000009, respectively).The risk was increased 2.4-fold in oral contraceptive users with a Glu/Gln genotype. We conclude that the risk of cervical cancer is increased in passive smokers and in users of oral contraceptives with certain NBS1 genotypes.

  15. Pulmonary lymphangitic carcinomatosis from squamous cell carcinoma of the cervix:A retrospective study with review of literature

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    Mamta Singla

    2016-01-01

    Full Text Available Background: Pulmonary lymphangitic carcinomatosis (LC secondary to cervical squamous cell carcinoma (SCC is an uncommon cause of diffuse infiltrative lung disease. Its reported incidence is quiet low. Materials and Methods: Fifty cases of cervical carcinoma were studied from 2005 to 2014. There was only one case of squamous cell carcinoma cervix that developed pulmonary lymphangitic carcinomatosis subsequently. Clinical manifestations of LC such as dyspnea and non-productive cough can mimic with clinical picture of pneumonia, pneumonitis, pulmonary embolism, congestive heart failure, asthma, and sarcoidosis and thus can be a diagnostic dilemma for treating physician. A review of world literature was also done to examine all the reported cases of cervical carcinoma which presented as pulmonary lymphangitic carcinomatosis. A few cases have been reported so far. Concussion: Pulmonary lymphangitic carcinomatosis is a rare manifestation of metastatic Squamous Cell Carcinoma (SCC of the cervix and is associated with a poor prognosis. Increased clinical alertness of such patterns of metastases in cervical cancer along with accurate pathological diagnosis is compulsory to guide proper therapy in these patients.

  16. Results from phase III clinical trials with radachlorine for photodynamic therapy of pre-cancer and early cancer of cervix

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    E. V. Filonenko

    2015-01-01

    Full Text Available The results of clinical study for efficacy of photodynamic therapy (PDT with radachlorine in patients with pre-cancer and cancer of cervix are represented. The study enrolled 30 patients including 4 patients with cervical erosion, 5 patients with cervical intraepithelial neoplasia II, 13 patients with cervical intraepithelial neoplasia III, 4 patients with carcinoma in situ and 4 patients with cervical cancer stage Ia. Radachlorine was administrated as single 30 minute intravenous injection at dose of 1,0 mg/kg of body weight 3 h before irradiation (wavelength of 662 nm, light dose of 300–350 J/cm2. The results of treatment in 26 (86,7% patients was assessed as complete tumor regression and in 4 (13,3% patients — as partial regression. In cervical erosion, intraepithelial neoplasia II and carcinoma in situ groups total regression was in all cases. In the cervical intraepithelial neoplasia III group total regression after first course of PDT was achieved in 77% of patients, in cervical cancer stage Ia group – in 75% of patients. From 3 to 6 months after first course of treatment all patients with partial tumor regression underwent the second course of PDT with complete regression. There were no side-effects due to radachorine or PDT in the course of treatment and during follow-up. Thus, PDT with Russian photosensitizer radachlorine showed high efficiency for treatment of pre-cancer and cancer of cervix

  17. Study of epidemiology of HPV infection in the Uterine Cervix of Women's in Delhi /NCR regions, India

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    Sharma Veena

    2012-03-01

    Full Text Available One of the most common cancers among Indian women is the cancer of cervix. Hence, the Present study is undertaken to determine the prevalence of HR-HPV DNA in women in Delhi / NCR regions. The significance of detection of HPV provides the base to be used as a tool to identify women, at the risk of subsequent development of cervical cancer. It is the utmost necessity to identify the prevalence of high risk- Human Papilloma Virus (HR-HPV in the women with cervical cytology for early treatment. Total of 1931 samples (cervical samples from different hospitals in Delhi / NCR regions of India were collected in between January 2006 to December 2009. The cervical cytobrush was used for collection of samples from cervix and then the samples were transported in virus transport media (Digene Diag, Md. Hybrid capture assay II (HCA II for HPV DNA detection from Digene Diagnostics (Silver Spring, Md. was used for the detection of High risk Human Papilloma Virus. High risk Human Papilloma Virus was detected in 232 cases (12.01%. As analyzed it was observed that positivity rate has increased for last few years, 9.49 %, 11.66 %, 11.94 %, 14.7 %, in the year 2006, 2007, 2008, and 2009 respectively.

  18. Implementation of image-guided brachytherapy (IGBT) for patients with uterine cervix cancer: a tumor volume kinetics approach

    Science.gov (United States)

    Mendez, Lucas Castro; Stuart, Silvia Radwanski; Guimarães, Roger Guilherme Rodrigues; Ramos, Clarissa Cerchi Angotti; de Paula, Lucas Assad; de Sales, Camila Pessoa; Chen, André Tsin Chih; Blasbalg, Roberto; Baroni, Ronaldo Hueb

    2016-01-01

    Purpose To evaluate tumor shrinking kinetics in order to implement image-guided brachytherapy (IGBT) for the treatment of patients with cervix cancer. Material and methods This study has prospectively evaluated tumor shrinking kinetics of thirteen patients with uterine cervix cancer treated with combined chemoradiation. Four high dose rate brachytherapy fractions were delivered during the course of pelvic external beam radiation therapy (EBRT). Magnetic resonance imaging (MRI) exams were acquired at diagnosis (D), first (B1), and third (B3) brachytherapy fractions. Target volumes (GTV and HR-CTV) were calculated by both the ellipsoid formula (VE) and MRI contouring (VC), which were defined by a consensus between at least two radiation oncologists and a pelvic expert radiologist. Results Most enrolled patients had squamous cell carcinoma and FIGO stage IIB disease, and initiated brachytherapy after the third week of pelvic external beam radiation. Gross tumor volume volume reduction from diagnostic MRI to B1 represented 61.9% and 75.2% of the initial volume, when measured by VE and VC, respectively. Only a modest volume reduction (15-20%) was observed from B1 to B3. Conclusions The most expressive tumor shrinking occurred in the first three weeks of oncological treatment and was in accordance with gynecological examination. These findings may help in IGBT implementation. PMID:27648083

  19. Increased number of IL-2, IL-2 receptor and IL-10 positive cells in premalignant lesions of the cervix.

    Science.gov (United States)

    Mindiola, Raimy; Caulejas, Diana; Núñez-Troconis, José; Araujo, Mary; Delgado, Mariela; Mosquera, Jesús

    2008-12-01

    Previous studies have shown the involvement of the immune response in the progression of human uterine cervix cancer. The aim of this study was to determine the expression of Interleukin-2 (IL-2), IL-2 receptor (IL-2R) and Interleukin 10 (IL-10) in different grades of cervical intraepithelial neoplasias of the exocervix (CIN 1, 2 and 3), and its relationship with the serum cytokine profiles and human papilomavirus (HPV) infection status. Indirect immunofluorescence was used to study the expression of IL-2, IL-2R and IL-10 in human cervical samples from 50 patients and 9 normal controls. Serum IL-2, IL-2R and IL-10 were measured by ELISA and HPV DNA and HPV types were identified by PCR. Increased number of IL-2, IL-2R and IL-10 positive cells were observed in the cervix from patients with CIN, associated with the grades of dysplasia. A significant correlation was observed between IL-2 and IL-2R (p>0.0001), IL-2 and IL-10 (p>0.0001), as well as IL-10 and IL-2R (p>0.0001). Twenty percent of patients were HPV positive and 84% of those patients were tissue cytokine positive. These results suggest that IL-2, IL-2R and IL-10 tissue expression may play a role in the development of cervical intraepithelial dysplasias.

  20. Radiation therapy in recurrence of carcinoma of the uterine cervix after primary surgery

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Jin Hee; Kim, Ok Bae [College of Medicine, Keimyung Univ., Daegu (Korea, Republic of)

    2003-06-01

    recurrent carcinoma of the uterine cervix following primary surgery, especially the external beam radiation and vaginal intracavitary irradiation achieved the best results in the patients with a vaginal cuff recurrence following primary surgery.

  1. Bladder–Rectum Spacer Balloon in High-Dose-Rate Brachytherapy in Cervix Carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Rai, Bhavana [Department of Radiotherapy and Oncology, Regional Cancer Centre, Postgraduate Institute of Medical Education and Research, Chandigarh (India); Patel, Firuza D., E-mail: firuzapatel@gmail.com [Department of Radiotherapy and Oncology, Regional Cancer Centre, Postgraduate Institute of Medical Education and Research, Chandigarh (India); Chakraborty, Santam; Sharma, Suresh C.; Kapoor, Rakesh [Department of Radiotherapy and Oncology, Regional Cancer Centre, Postgraduate Institute of Medical Education and Research, Chandigarh (India); Aprem, Abi Santhosh [Corporate R and D Division, HLL Lifecare Limited, Karamana, Trivandrum (India)

    2013-04-01

    Purpose: To compare bladder and rectum doses with the use of a bladder–rectum spacer balloon (BRSB) versus standard gauze packing in the same patient receiving 2 high-dose-rate intracavitary brachytherapy fractions. Methods and Materials: This was a randomized study to compare the reduction in bladder and rectum doses with the use of a BRSB compared with standard gauze packing in patients with carcinoma of the cervix being treated with high-dose-rate intracavitary brachytherapy. The patients were randomized between 2 arms. In arm A, vaginal packing was done with standard gauze packing in the first application, and BRSB was used in the second application. Arm B was the reverse of arm A. The International Commission for Radiation Units and Measurement (ICRU) point doses and doses to 0.1-cm{sup 3}, 1-cm{sup 3}, 2-cm{sup 3}, 5-cm{sup 3}, and 10-cm{sup 3} volumes of bladder and rectum were compared. The patients were also subjectively assessed for the ease of application and the time taken for application. Statistical analysis was done using the paired t test. Results: A total of 43 patients were enrolled; however, 3 patients had to be excluded because the BRSB could not be inserted owing to unfavorable local anatomy. Thus 40 patients (80 plans) were evaluated. The application was difficult in 3 patients with BRSB, and in 2 patients with BRSB the application time was prolonged. There was no significant difference in bladder doses to 0.1 cm{sup 3}, 1 cm{sup 3}, 2 cm{sup 3}, 5 cm{sup 3}, and 10 cm{sup 3} and ICRU bladder point. Statistically significant dose reductions to 0.1-cm{sup 3}, 1-cm{sup 3}, and 2-cm{sup 3} volumes for rectum were observed with the BRSB. No significant differences in 5-cm{sup 3} and 10-cm{sup 3} volumes and ICRU rectum point were observed. Conclusion: A statistically significant dose reduction was observed for small high-dose volumes in rectum with the BRSB. The doses to bladder were comparable for BRSB and gauze packing. Transparent balloons of

  2. A novel method to quantify and compare anatomical shape: application in cervix cancer radiotherapy

    Science.gov (United States)

    Oh, Seungjong; Jaffray, David; Cho, Young-Bin

    2014-06-01

    Adaptive radiation therapy (ART) had been proposed to restore dosimetric deficiencies during treatment delivery. In this paper, we developed a technique of Geometric reLocation for analyzing anatomical OBjects' Evolution (GLOBE) for a numerical model of tumor evolution under radiation therapy and characterized geometric changes of the target using GLOBE. A total of 174 clinical target volumes (CTVs) obtained from 32 cervical cancer patients were analyzed. GLOBE consists of three main steps; step (1) deforming a 3D surface object to a sphere by parametric active contour (PAC), step (2) sampling a deformed PAC on 642 nodes of icosahedron geodesic dome for reference frame, and step (3) unfolding 3D data to 2D plane for convenient visualization and analysis. The performance was evaluated with respect to (1) convergence of deformation (iteration number and computation time) and (2) accuracy of deformation (residual deformation). Based on deformation vectors from planning CTV to weekly CTVs, target specific (TS) margins were calculated on each sampled node of GLOBE and the systematic (Σ) and random (σ) variations of the vectors were calculated. Population based anisotropic (PBA) margins were generated using van Herk's margin recipe. GLOBE successfully modeled 152 CTVs from 28 patients. Fast convergence was observed for most cases (137/152) with the iteration number of 65 ± 74 (average ± STD) and the computation time of 13.7 ± 18.6 min. Residual deformation of PAC was 0.9 ± 0.7 mm and more than 97% was less than 3 mm. Margin analysis showed random nature of TS-margin. As a consequence, PBA-margins perform similarly to ISO-margins. For example, PBA-margins for 90% patients' coverage with 95% dose level is close to 13 mm ISO-margins in the aspect of target coverage and OAR sparing. GLOBE demonstrates a systematic analysis of tumor motion and deformation of patients with cervix cancer during radiation therapy and numerical modeling of PBA-margin on 642 locations of CTV

  3. Intra-patient semi-automated segmentation of the cervix-uterus in CT-images for adaptive radiotherapy of cervical cancer

    NARCIS (Netherlands)

    L. Bondar (Luiza); M.S. Hoogeman (Mischa); W. Schillemans; B.J.M. Heijmen (Ben)

    2013-01-01

    textabstractFor online adaptive radiotherapy of cervical cancer, fast and accurate image segmentation is required to facilitate daily treatment adaptation. Our aim was twofold: (1) to test and compare three intra-patient automated segmentation methods for the cervix-uterus structure in CT-images and

  4. Intracervical PGE2 gel for induction of labour in patients with prelabour rupture of membranes with unfavorable cervix after 34 weeks period of gestation

    Directory of Open Access Journals (Sweden)

    Sheela Jayaprakash

    2016-05-01

    Conclusions: Intra-cervical PGE2 gel is safe and effective for inducing labour in patients with PROM with unfavorable cervix. In our study we had high rate of vaginal delivery with no infectious morbidity. [Int J Reprod Contracept Obstet Gynecol 2016; 5(5.000: 1418-1422

  5. Pseudo tumor tuberculosis of the uterine cervix: about a case at the colposcopy and cervico vaginal pathologies unit of the University hospital Le Dantec, Dakar, Senegal

    Directory of Open Access Journals (Sweden)

    Omar Gassama

    2016-08-01

    Full Text Available Tuberculosis is still common in developing countries and particularly in sub-Saharan Africa since the advent of the HIV/AIDS. Genital tuberculosis usually affects young women in genital activity period. The most frequent locations are tubal, endometrial and ovarian. The cervical location is rare. We report on the case of a 36 years old patient with eight pregnancies and eight deliveries who lives in a rural area and has got in her history 8 vaginal deliveries with four living children and 4 dead children and who was referred by a colleague for a "tumor of the uterine cervix". In her medical history, there was a BCG vaccination during childhood and she had never received Pap smear. The colposcopy revealed an ulcerating budding tumor of the cervix with necrotic areas. The colposcopy biopsy revealed fibrocaseous tuberculosis of the uterine cervix. Tuberculosis is still a common disease in developing countries. The cervical localization is rare but should be considered in case of an ulcerating tumor budding of the cervix. [Int J Reprod Contracept Obstet Gynecol 2016; 5(8.000: 2840-2843

  6. Müllerian adenosarcoma of the uterine cervix with sarcomatous overgrowth: A case report of aggressive disease in a young patient

    Directory of Open Access Journals (Sweden)

    David A. Morales F.

    2016-01-01

    Conclusion: A young woman with Müllerian adenosarcoma of the cervix with sarcomatous overgrowth presenting the risk factors for its recurrence experienced a rapid relapse after receiving radical surgery but not adjuvant therapy. Control of this aggressive disease via sequential radiotherapy and chemotherapy are recommended.

  7. Progress in research of vagina micro ecological imbalance in Chinese and Western medicine%阴道微生态失调的中西医研究进展

    Institute of Scientific and Technical Information of China (English)

    王丹丹; 董燕

    2014-01-01

    Vaginal micro ecological imbalance refers to the normal flora imbalance of the vagina, including the result of the impact of external factors , the mutual restraint and interdependent among the original flora to broke the balance , causing abnormal vaginal discharge, genital itching or waist, a gynecological abdominal pain symptoms of vaginitis, vaginal as female genital connection with the outside world the only passage between the environment, the human body is an important micro­ecological zones. Is also an important part of the reproductive tract micro flora. In the vaginal wall mucosal folds and then there is the vault vaginal flora, usually vaginal flora with lactobacilli was the dominant bacteria. Into a variety of lactic acid bacteria, Lactobacillus probiotics for the maintenance of the vagina is important, PH value of the vagina can be maintained in the normal acidic condition, can also inhibit bacteria play a bactericidal effect, in addition to lactic acid bacteria in the vagina outside of further there are fungi, viruses, etc., under normal circumstances, a variety of flora in the vagina interdependent mutual restraint, joint action within the vaginal environment will be maintained at a balanced state, the dominant strain of Lactobacillus, once lost the advantage will lead to other flora overgrowth, breaking the vaginal micro flora balance, leading to female vaginal dysbiosis.%阴道微生态失调是指阴道中的正常的菌群在内因或在外因的影响下出现了菌群之间的失衡,使原来菌群之间的互相制约、互相依赖的平衡关系被打破,从而引起白带异常、外阴瘙痒或是腰部、下腹部疼痛[1]的阴道炎症状的一种妇科疾病。阴道作为连接女性内生殖器与外界环境之间唯一的通道,是人体内重要的微生态区,也是生殖道微生态的重要部分。在阴道的侧壁黏膜、皱襞,而后是穹窿都存在有阴道菌群,通常阴道菌群

  8. Anatomicohistological characteristics of the tubular genital organs of the female collared peccary (Tayassu tajacu) from North-eastern Amazon.

    Science.gov (United States)

    Mayor, P; Jori, F; López-Béjar, M

    2004-04-01

    The present study examines anatomical and histological characteristics of tubular genital organs and its relationships with the reproductive state of 24 wild adult collared peccary (Tayassu tajacu) females. The tunica mucosa of the uterine tube presents a pseudostratified, intermittently ciliated columnar epithelium. The epithelial secretory cells of pregnant females and females in the luteal phase of the oestrous cycle became taller than the ciliated cells and showed abundant apical secretory blebs, whereas secretory cells of females in the follicular phase showed abundant mucous secretory activity (periodic acid-Schiff positive cells). The uterus is composed of two narrow and convoluted uterine horns, separated by the velum uteri, a small uterine body and a long and muscular cervix. The endometrial lining of both uterine horns and body is a monostratified, columnar ciliated epithelium. Pregnant females and females in luteal phase showed a more developed hyperplasia of the endometrial simple tubular glands than females in the follicular phase. The cervix presents interdigitated rows of mucosal prominences that project into the lumen, structures similar to pulvini cervicali, occluding the cervical canal. In pregnant females, the endocervical canal was filled by a viscous cervical secretion. Females in follicular phase presented a thicker vaginal epithelium than pregnant females and females in luteal phase. The present study suggests that the collared peccary female showed different histological features of the uterine tubes, uterus and vagina in accordance with the reproductive state of the females.

  9. Utility of Ki-67 and p53 in distinguishing cervical intraepithelial neoplasia 3 from squamous cell carcinoma of the cervix.

    Science.gov (United States)

    Tan, Geok Chin; Sharifah, Noor Akmal; Shiran, Mohd Sidik; Salwati, Shuib; Hatta, Ahmad Zailani; Paul-Ng, Hock Oon

    2008-01-01

    The differentiation between cervical intraepithelial neoplasia 3 (CIN 3) and early squamous cell carcinoma (SCC) of the cervix may be difficult in certain situations. Identification of invasion beyond the basement membrane is the gold standard for the diagnosis of the latter. The objective of this study was to determine whether the use of Ki-67 and p53 could help in solving the above dilemma. This was a retrospective study on 61 cases of cervical neoplasms comprising of 25 cases of CIN 3 and 36 SCC. All cases were evaluated by immunohistochemistry using Ki-67 and p53 monoclonal antibodies. Results showed that the differences of Ki-67 and p53 expression between CIN 3 and SCC were statistically significant. In conclusion, Ki-67 and p53 may serve as helpful adjuncts to routinely-stained histological sections in differentiating between CIN 3 and SCC.

  10. Diagnosing Cervical Dysplasia Using Visual Inspection of the Cervix with Acetic Acid in a Woman in Rural Haiti

    Directory of Open Access Journals (Sweden)

    Elizabeth Roger

    2014-11-01

    Full Text Available Cervical cancer remains a significant cause of morbidity and mortality for women in developing countries, despite the fact that inexpensive, simple and effective screening methods are available. Visual inspection of the cervix with acetic acid (VIA can be used as part of a “screen and treat” program to identify precancerous lesions for cryotherapy treatment. This case report details how the VIA screening test was incorporated into the care of a patient presenting to a maternal health clinic in Thomonde, Haiti which was staffed by doctors and medical students from Emory University School of Medicine in collaboration with Haiti Medishare. As demonstrated here, the VIA test requires minimal materials, can be efficiently incorporated into a physical exams, provides immediate results, and is easily demonstrated to and performed by local healthcare providers. The straightforward and sensitive VIA technique is an ideal cervical cancer screening method for resource poor areas.

  11. A study of two protocols combining aglepristone and cloprostenol to treat open cervix pyometra in the bitch.

    Science.gov (United States)

    Gobello, Cristina; Castex, Gervasio; Klima, Liliana; Rodríguez, Raúl; Corrada, Yanina

    2003-09-15

    To compare the efficacy and safety of two protocols using a combination of aglepristone and cloprostenol for the treatment of open cervix pyometra in the bitch and to describe the progesterone (P4) serum profiles before and during treatments, 15 bitches were randomly allocated into two treatment groups: I (n = 8): aglepristone was administered at 10mg/kg, s.c., on Days 1, 3, 8, and 15 (if not cured), combined with cloprostenol at the dose of 1 microg/kg, s.c., on Days 3 and 8, and II (n = 7): received the same treatment with aglepristone as Treatment I but cloprostenol on Days 3, 5, 8 10, 12, and 15 (if not cured). Before the beginning of the treatments and then on Days 8, 15, and 29 all bitches were evaluated for clinical signs, side effects, hemogram, serum P4 concentrations, and uterus diameters. Bitches in both treatment groups, with (n = 6) or without (n = 9; > or =1.2 ng/ml) initial basal P4 serum concentrations, achieved treatment success without side effects and no significant differences, either on Day 15 (6/8 for Treatment I and 4/7 for Treatment II) or on Day 29 (2/8 for Treatment I and 3/7 for Treatment II). In both treatments groups, clinical signs, blood parameters, and uterine diameters improved to normal values throughout the experiments. A significant interaction between day and treatment was found for percentage change in P4 when all bitches were considered together. Redevelopment of pyometra in the next estrous cycle occurred in 20% of the bitches. One nonrecurrent bitch was mated and whelped a normal litter. It is concluded that these two combined protocols proved to be efficient and safe in reversing clinical signs of open cervix pyometra independently of initial P4 concentrations and that the number of cloprostenol administrations seemed to have an effect on P4 serum changes throughout treatments.

  12. Small bowel sparing effect of small bowel displacement system in 3D-CRT and IMRT for cervix cancer

    Energy Technology Data Exchange (ETDEWEB)

    Kang, Min Kyu; Huh, Seung Jae; Han, Young Yih [Sungkyunkwan University School of Medicine, Seoul (Korea, Republic of)] (and others)

    2004-06-15

    In radiotherapy for cervix cancer, both 3-dimensional radiotherapy (3D-CRT) and intensity-modulated radiation therapy (IMRT) could reduce the dose to the small bowel (SB), while the small bowel displacement system (SBDS) could reduce the SB volume in the pelvic cavity. To evaluate the effect of the SBDS on the dose to the SB in 3D-CRT and IMRT plans, 3D-CRT and IMRT plans, with or without SBDS, were compared. Ten consecutive uterine cervix cancer, receiving curative radiotherapy, were accrued. Ten pairs of computerized tomography (CT) scans were obtained in the prone position, with or without SBDS, which consisted of a Styrofoam compression device and an individualized custom-made abdominal immobilization device. Both 3D-CRT, using the 4-field box technique, and IMRT plans, with 7 portals of 15 MV X-ray, were generated for each CT image, and prescribed 50 Gy (25 fractions) to the isocenter. For the SB, the volume change due to the SBDS and the DVHs of the four different plans were analyzed using paired t-tests. The SBDS significantly reduced the mean SB volume from 522 to 262 cm{sup 3} (49.8% reduction). The SB volumes that received a dose of 10 {approx} 50 Gy were significantly reduced in 3D-CRT (65 {approx} 80% reduction) and IMRT plans (54 {approx} 67% reduction) using the SBDS. When the SB volumes that received 20 {approx} 50 Gy were compared between the 3D-CRT and IMRT plans, those of the IMRT without the SBDS were significantly less, by 6{approx} 7%, than those for the 3D-CRT without the SBDS, but the volume difference was less than 1% when using the SBDS. The SBDS reduced the radiation dose to the SB in both the 3D-CRT and IMRT plans, so could reduce the radiation injury of the SB.

  13. HPV Genotypes Predict Survival Benefits From Concurrent Chemotherapy and Radiation Therapy in Advanced Squamous Cell Carcinoma of the Cervix

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Chun-Chieh [Department of Radiation Oncology, Chang Gung Memorial Hospital, Taoyuan, Taiwan (China); Department of Medical Imaging and Radiological Science, Chang Gung University, School of Medicine, Taoyuan, Taiwan (China); Lai, Chyong-Huey [Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Taoyuan, Taiwan (China); Huang, Yi-Ting [Department of Radiation Oncology, Chang Gung Memorial Hospital, Taoyuan, Taiwan (China); Chao, Angel; Chou, Hung-Hsueh [Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Taoyuan, Taiwan (China); Hong, Ji-Hong, E-mail: jihong@adm.cgmh.org.tw [Department of Radiation Oncology, Chang Gung Memorial Hospital, Taoyuan, Taiwan (China); Department of Medical Imaging and Radiological Science, Chang Gung University, School of Medicine, Taoyuan, Taiwan (China)

    2012-11-15

    Purpose: To study the prognostic value of human papillomavirus (HPV) genotypes in patients with advanced cervical cancer treated with radiation therapy (RT) alone or concurrent chemoradiation therapy (CCRT). Methods and Materials: Between August 1993 and May 2000, 327 patients with advanced squamous cell carcinoma of the cervix (International Federation of Gynecology and Obstetrics stage III/IVA or stage IIB with positive lymph nodes) were eligible for this study. HPV genotypes were determined using the Easychip Registered-Sign HPV genechip. Outcomes were analyzed using Kaplan-Meier survival analysis and the Cox proportional hazards model. Results: We detected 22 HPV genotypes in 323 (98.8%) patients. The leading 4 types were HPV16, 58, 18, and 33. The 5-year overall and disease-specific survival estimates for the entire cohort were 41.9% and 51.4%, respectively. CCRT improved the 5-year disease-specific survival by an absolute 9.8%, but this was not statistically significant (P=.089). There was a significant improvement in disease-specific survival in the CCRT group for HPV18-positive (60.9% vs 30.4%, P=.019) and HPV58-positive (69.3% vs 48.9%, P=.026) patients compared with the RT alone group. In contrast, the differences in survival with CCRT compared with RT alone in the HPV16-positive and HPV-33 positive subgroups were not statistically significant (P=.86 and P=.53, respectively). An improved disease-specific survival was observed for CCRT treated patients infected with both HPV16 and HPV18, but these differenced also were not statistically significant. Conclusions: The HPV genotype may be a useful predictive factor for the effect of CCRT in patients with advanced squamous cell carcinoma of the cervix. Verifying these results in prospective trials could have an impact on tailoring future treatment based on HPV genotype.

  14. An intrauterine ultrasound applicator for targeted delivery of thermal therapy in conjunction with HDR brachytherapy to the cervix

    Science.gov (United States)

    Wootton, Jeffery H.; Juang, Titania; Pouliot, Jean; Hsu, I.-Chow Joe; Diederich, Chris J.

    2009-02-01

    An intracavitary hyperthermia applicator for targeted heat delivery to the cervix was developed based on a linear array of sectored tubular ultrasound transducers that provides truly 3-D heating control (angular and along the length). A central conduit can incorporate an HDR source for sequential or simultaneous delivery of heat and radiation. Hyperthermia treatment volumes were determined from brachytherapy treatment planning data and used as a basis for biothermal simulations analyzing the effects of device parameters, tissue properties, and catheter materials on heating patterns. Devices were then developed with 1-3 elements at 6.5-8 MHz with 90-180° sectors and a 15-35 mm heating length, housed within a 6-mm diameter water-cooled PET catheter. Directional heating from sectored transducers could extend lateral penetration of therapeutic heating (41°C) >2 cm while maintaining rectum and bladder temperatures within 12 mm below thermal damage thresholds. Imaging artifacts were evaluated with standard CT, cone beam CT, and MR images. MR thermal imaging was used to demonstrate shaping of heating profiles in axial and coronal slices with artifact <2 mm from the device. The impact of the high-Z applicator materials on the HDR dose distribution was assessed using a well-type ionization chamber and was found to be less than 6% attenuation, which can readily be accounted for with treatment planning software. The intrauterine ultrasound device has demonstrated potential for 3-D conformal heating of clinical tumors in the delivery of targeted hyperthermia in conjunction with brachytherapy to the cervix.

  15. Cervix carcinomas: place of intensity-modulated radiotherapy; Les cancers du col uterin: place de la radiotherapie avec modulation d'intensite

    Energy Technology Data Exchange (ETDEWEB)

    Barillot, I. [Centre Regional Universitaire de Cancerologie H.S.-Kaplan, Hopital Bretonneau, CHU de Tours, 37 - Tours (France); Universite Francois-Rabelais, 37 - Tours (France)

    2009-10-15

    While indications of modulated intensity radiation therapy (I.M.R.T.) are perfectly defined in head and neck and prostate cancer patients, this technique remains under evaluation for gynecologic tumours. The implementation of conformal three dimensional radiotherapy in the late 1990 has been the first important step for optimisation of treatment of cervix carcinomas, as it permitted a better target coverage with a significant reduction of the bladder dose. However, this technique often leads to an irradiation of a larger volume of rectum in locally advanced stages and could only spare a limited amount of intestine. I.R.M.T. is one of the optimisation methods potentially efficient for a better sparing of digestive tract during irradiation of cervix carcinomas. The aim of this literature review is to provide the arguments supporting this hypothesis, and to define the place of this technique for dose escalation. (authors)

  16. Positive correlation between patency and mRNA levels for cyclooxygenase-2 and prostaglandin E synthase in the uterine cervix of bitches with pyometra.

    Science.gov (United States)

    Tamada, Hiromichi; Adachi, Nahoko; Kawate, Noritoshi; Inaba, Toshio; Hatoya, Shingo; Sawada, Tsutomu

    2016-03-01

    Factors involved in patency of uterine cervices in the bitch with pyometra remain to be clarified. This study examined relationship between patency and mRNA levels for inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-1, COX-2 and prostaglandin E synthase (PGES) in the uterine cervix of bitches with pyometra. Cervical patency was measured by inserting the stainless steel rods with different diameter into cervical canals. Levels of mRNA expression were determined by semi-quantitative reverse transcription-polymerase chain reaction. The cervical patency was positively correlated with mRNA levels for COX-2 and PGES, but not those for iNOS and COX-1. The results suggest that gene expression of COX-2 and PGES may be involved in the regulation of patency in the uterine cervix of bitches with pyometra.

  17. Early diffusion weighted magnetic resonance imaging can predict survival in women with locally advanced cancer of the cervix treated with combined chemo-radiation

    Energy Technology Data Exchange (ETDEWEB)

    Somoye, Gbolahan; Parkin, David [Ward 42, Aberdeen Royal Infirmary, Aberdeen (United Kingdom); Harry, Vanessa [Royal Marsden NHS Foundation Trust, London (United Kingdom); Semple, Scott [Queen' s Medical Research Institute, Centre for Cardiovascular Science, Clinical Research Imaging Centre, Edinburgh (United Kingdom); Plataniotis, George [Musgrove Park Hospital, Taunton and Somerset NHS Foundation, Taunton (United Kingdom); Scott, Neil [University of Aberdeen, Section of Population Health, Aberdeen (United Kingdom); Gilbert, Fiona J. [University of Cambridge, Radiology Department, Box 218, Cambridge (United Kingdom)

    2012-11-15

    To assess the predictive value of diffusion weighted imaging (DWI) for survival in women treated for advanced cancer of the cervix with concurrent chemo-radiotherapy. Twenty women treated for advanced cancer of the cervix were recruited and followed up for a median of 26 (range <1 to 43) months. They each had DWI performed before treatment, 2 weeks after beginning therapy (midtreatment) and at the end of treatment. Apparent diffusion coefficient (ADC) values were calculated from regions of interest (ROI). All participants were reviewed for follow-up data. ADC values were compared with mortality status (Mann-Whitney test). Time to progression and overall survival were assessed (Kaplan-Meier survival graphs). There were 14 survivors. The median midtreatment ADC was statistically significantly higher in those alive compared to the non-survivors, 1.55 and 1.36 (x 10{sup -3}/mm{sup 2}/s), respectively, P = 0.02. The median change in ADC 14 days after treatment commencement was significantly higher in the alive group compared to non-survivors, 0.28 and 0.14 (x 10{sup -3}/mm{sup 2}/s), respectively, P = 0.02. There was no evidence of a difference between survivors and non-survivors for pretreatment baseline or post-therapy ADC values. Functional DWI early in the treatment of advanced cancer of the cervix may provide useful information in predicting survival. (orig.)

  18. Human papillomavirus (HPV infection and intraepithelial neoplasia and invasive cancer of the uterine cervix: a case-control study in Zaragoza, Spain

    Directory of Open Access Journals (Sweden)

    Moros Manuel

    2008-05-01

    Full Text Available Abstract Introduction The raw incidence of cancer of the uterine cervix is Spain is 7,8 per 100.000 inhabitants (adjusted incidence is 5.6. The incidence of this tumor is still low, but a steady increase has been seen, probably related to increasing risk factors. Aim To determine the frequency of infection by different types of human papillomavirus (HPV in Papanicolau smears from women with and without cancer of the uterine cervix in Spain. Patients and methods A case-control study was performed in women with and without cervical cancer from Zaragoza, Spain. Pap smears from 600 cases (540 women with cervical intraepithelial neoplasms (CIN and 60 with invasive cancer and 1200 controls (women without those lesions were tested by polymerase chain reaction (PCR and typed by oligonucleotide microarray-based detection. Results HPV was detected in 93.3% of all samples with invasive cancer versus 17.5% of controls. OR for invasive cancer was 55 (95% CI 21.5–140,5. Statistically significant associations were also found for different grades of cervical dysplasia. Conclusion The strong association found between HPV infection, specifically types 16 and 18 and cancer of the uterine cervix in Zaragoza, Spain, stresses the importance of ongoing efforts to institute a vaccine program with recently approved HPV vaccines in order to prevent cervical cancer in this population.

  19. Challenging the in-vivo assessment of biomechanical properties of the uterine cervix: A critical analysis of ultrasound based quasi-static procedures.

    Science.gov (United States)

    Maurer, M M; Badir, S; Pensalfini, M; Bajka, M; Abitabile, P; Zimmermann, R; Mazza, E

    2015-06-25

    Measuring the stiffness of the uterine cervix might be useful in the prediction of preterm delivery, a still unsolved health issue of global dimensions. Recently, a number of clinical studies have addressed this topic, proposing quantitative methods for the assessment of the mechanical properties of the cervix. Quasi-static elastography, maximum compressibility using ultrasound and aspiration tests have been applied for this purpose. The results obtained with the different methods seem to provide contradictory information about the physiologic development of cervical stiffness during pregnancy. Simulations and experiments were performed in order to rationalize the findings obtained with ultrasound based, quasi-static procedures. The experimental and computational results clearly illustrate that standardization of quasi-static elastography leads to repeatable strain values, but for different loading forces. Since force cannot be controlled, this current approach does not allow the distinction between a globally soft and stiff cervix. It is further shown that introducing a reference elastomer into the elastography measurement might overcome the problem of force standardization, but a careful mechanical analysis is required to obtain reliable stiffness values for cervical tissue. In contrast, the maximum compressibility procedure leads to a repeatable, semi-quantitative assessment of cervical consistency, due to the nonlinear nature of the mechanical behavior of cervical tissue. The evolution of cervical stiffness in pregnancy obtained with this procedure is in line with data from aspiration tests.

  20. [Stage III cancer of the cervix. The diagnosis, treatment and prognosis in a series of 92 patients (author's transl)].

    Science.gov (United States)

    Heintz, J

    1980-01-01

    This study is of 92 patients who were treated for Stage III carcinoma of the cervix in the Henri Becquerel Centre. We have ruled out the 11 patients who refused to complete their treatment or who died before treatment started. The average age was 63 years. The Stage III cases were divided into 40 Stage III A and 41 Stage III B, with 10 who had urinary tract involvement. These were epidermoid carcinomata. The treatment was by external radiotherapy followed by superimposed radium therapy in 66 cases. 15 patients were treated by external radiotherapy alone. The lumboartic nodes were treated in the area that was irradiated in 5 patients. The actuarial figures for survival work out at 51 per cent at 5 years for Stage III A cases and 34 per cent for Stage III B cases. Pelvic recurrences were responsible for 87 per cent of the failures. In fact, in almost 87 per cent of the cases the area was not completely sterilised. The percentage of failures was higher in the group that had altered lymphograms. The prognosis was worst when the upper urinary tract was affected. All patients who had positive lymphograms or those that were suspicious of having lumboaortic nodes involved died. In the 15 patients who did not have added radium therapy there were 13 cases who were not sterilised and 1 that recurred at 13 months. In 32 per cent of cases the failures in the pelvis were associated with pathology in distant lymph nodes and/or with visceral metastases. We found 13 per cent of solitary metastases. Between 5 and 10 years the failure rate is 9 per cent with a level of 6.3 per cent of pelvic recurrences associated or not associated with metastases or even with complications. Our patients had 8 rectosigmoid complications of which 2 needed a diversion colostomy. There were 7 cases of cystitis after X-ray, in the majority of a benign nature, and 2 bony complications that recovered spontaneously. After a study of the diagnosis and treatment of Stage III cancer of the cervix, the

  1. Conservative treatment of coexisting microinvasive squamous and adenocarcinoma of the cervix: report of two cases and literature review

    Directory of Open Access Journals (Sweden)

    Sopracordevole F

    2016-01-01

    Full Text Available Francesco Sopracordevole,1,* Jacopo Di Giuseppe,2,* Silvia Cervo,3,4 Monica Buttignol,1 Giorgio Giorda,1 Andrea Ciavattini,2 Vincenzo Canzonieri3,5 1Gynecologic Oncology Unit, Department of Surgical Oncology, CRO Aviano National Cancer Institute, Aviano, Pordenone, 2Woman’s Health Sciences Department, Gynaecologic Section, Polytechnic University of Marche, Ancona, 3CRO-Biobank, 4Clinical Cancer Pathology, 5Pathology Unit, CRO Aviano National Cancer Institute, Aviano, Pordenone, Italy *These authors contributed equally to this work Abstract: Coexistence of microinvasive squamous cell carcinoma (MISCC and microinvasive adenocarcinoma (MIAC of the cervix is a rare phenomenon with very few clinically significant cases described in the literature. While a conservative approach has been studied, and may be effective in MISCC, a lower number of studies that recommend conservative treatment are available for MIAC. We report two cases of synchronous cervix lesions in two separate foci, MISCC and MIAC, who underwent fertility-sparing treatment with long-term follow-up. We describe clinical, histological, and immunohistochemical features of the two cases. The first case is a 41-year-old female with a diagnosis of MIAC of endocervical type, grade 1 differentiation, with a stromal invasion, associated with a separate area of squamous cell carcinoma (International Federation of Gynecology and Obstetrics/TNM stage: pT1a1G1. The second case is a 45-year-old female with a diagnosis of plurifocal MISCC, associated with an MIAC of endocervical type with a stromal invasion (International Federation of Gynecology and Obstetrics/TNM stage: pT1a1G1. After multidisciplinary counseling, both patients accepted conization as definitive treatment. Eleven years after the conization, all tests (Papanicolaou smear, colposcopy, cervical curettage, and hybrid capture 2-human papillomavirus test planned quarterly in the first year and every 6 months in the subsequent years

  2. Sympathetic innervation of the upper and lower regions of the uterus and cervix in the rat have different origins and routes.

    Science.gov (United States)

    Houdeau, E; Rousseau, A; Meusnier, C; Prud'Homme, M J; Rousseau, J P

    1998-09-28

    The origins and routes of the postganglionic sympathetic nerve supply to the upper and lower uterus and to the cervix were investigated in the rat by using denervation procedures combined with immunohistochemistry and retrograde tracing. The sympathetic nerve fibers of the upper part of the uterus arise from the ovarian plexus nerve. They mainly originate (90%) from neurons of the suprarenal ganglia (SRG) and of the T10 to L3 ganglia of the paravertebral sympathetic chain. Fluoro-Gold injections into different regions of the upper uterus showed that the SRG neurons mainly provide innervation to the tubal extremity (52%) rather than to the uterine portion below this area (26%). Very few neurons of the celiac ganglion or the aorticorenal ganglia participated in this innervation. Most of the sympathetic innervation of the lower uterus and the cervix (90%) originates from neurons of the paravertebral ganglia T13 to S2, principally at the L2-L4 levels. By using immunocytochemistry, we show that very few tyrosine hydroxylase-positive neurons of the pelvic plexus project to these areas, where they represent only 3% of the sympathetic nerve supply. Again, very few neurons of the inferior mesenteric ganglion (IMG) supply the lower uterus and the cervix. The comparison between retrograde tracing experiments in intact animals and after the removal of the IMG shows that very few sympathetic postganglionic axons from the paravertebral chain pass through the IMG to reach the lower uterus and the cervix. In contrast, these axons mainly project to splanchnic nerves bypassing the IMG to connect with the hypogastric nerves. In addition, some axons supplying the lower uterus follow the superior vesical arteries and then reach the organ. Taken together, these results show that the upper region of the uterus receives a sympathetic innervation that is different in origin and route from that of the lower uterus and the cervix. Such a marked region-specific innervation suggests that nerve

  3. A retrospective study of the effects of pelvic irradiation for carcinoma of the cervix on gastrointestinal function

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    Yeoh, E.; Ahmad, A.; Horowitz, M.; Russo, A.; Muecke, T.; Chatterton, B. (Royal Adelaide Hospital (Australia)); Robb, T. (Adelaide Children' s Hospital (Australia))

    1993-03-20

    The purpose of the study was to evaluate the prevalence of disordered gastrointestinal function following therapeutic irradiation. Gastrointestinal function was evaluated in 30 randomly selected patients who had received pelvic irradiation for treatment of carcinoma of the cervix between 1 and 6 years previously. Each patient underwent evaluations of (a) gastrointestinal symptoms (b) absorption of bile acid, vitamin B12, lactose and fat (c) gastrointestinal transit: gastric emptying, small intestinal transit and whole gut transit and (d) intestinal permeability. Results were compared with those obtained in 18 normal volunteers. Stool frequency was above the control range in five patients and had increased (p < 0.001) since radiotherapy treatment. Bile acid (p<0.001) vitamin B12(p<0.01) and lactose (p<0.01) absorption were less in the patients when compared with the control subjects. Bile acid adsorption was below the control range in 14 of the 30 patients. Dietary calcium intake was lower (p<0.05) in those patients with lactose malabsorption. Gastric emptying (p<0.01) and small intestinal transit (p<0.01) were more rapid in the patients. Both small intestinal (r=-0.39, p<0.05) and whole gut (r=-0.45) transit were inversely related to stool frequency. Either bowel frequency, bile acid adsorption, vitamin B12 absorption was outside the control range in 19 of the 30 patients. Abnormal gastrointestinal function is essentially an inevitable long-term sequel of pelvic irradiation. 41 refs., 4 figs., 3 tabs.

  4. Preoperative external beam radiotherapy and reduced dose brachytherapy for carcinoma of the cervix: survival and pathological response

    Directory of Open Access Journals (Sweden)

    Pellizzon Antonio

    2007-02-01

    Full Text Available Abstract Purpose To evaluate the pathologic response of cervical carcinoma to external beam radiotherapy (EBRT and high dose rate brachytherapy (HDRB and outcome. Materials and methods Between 1992 and 2001, 67 patients with cervical carcinoma were submitted to preoperative radiotherapy. Sixty-five patients were stage IIb. Preoperative treatment included 45 Gy EBRT and 12 Gy HDRB. Patients were submitted to surgery after a mean time of 82 days. Lymphadenectomy was performed in 81% of patients. Eleven patients with residual cervix residual disease on pathological specimen were submitted to 2 additional insertions of HDRB. Results median follow up was 72 months. Five-year cause specific survival was 75%, overall survival 65%, local control 95%. Complete pelvic pathological response was seen in 40%. Surgery performed later than 80 days was associated with pathological response. Pelvic nodal involvement was found in 12%. Complete pelvic pathological response and negative lymphnodes were associated with better outcome (p = .03 and p = .005. Late grade 3 and 4 urinary and intestinal adverse effects were seen in 12 and 2% of patients. Conclusion Time allowed between RT and surgery correlated with pathological response. Pelvic pathological response was associated with improved outcome. Postoperative additional HDRB did not improve therapeutic results. Treatment was well tolerated.

  5. The role of surgery in locally advanced carcinoma of cervix after sub-optimal chemoradiation: Indian scenario

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    Rajshekar S Kundargi

    2013-01-01

    Full Text Available Background: Standard treatment of advanced cervical cancer is concurrent chemoradiation. Radical radiotherapy for carcinoma cervix includes pelvic external beam radiotherapy (EBRT with the concomitant platinum based chemotherapy followed by intracavitary brachytherapy (ICBT to boost central disease. Management of patients who are suboptimally treated, especially, after unsuccessful ICBT insertion is not well-defined. This study explores the role of hysterectomy in these patients. Materials and Methods: From January 2006 to December 2011, 38 patients with locally advanced cervical cancer, in whom ICBT insertion was unsuccessful, were analyzed retrospectively. Operable patients with no parametrial involvement underwent hysterectomy and outcomes (recurrence free and overall survival were noted. Results: The major complications in post operative period were wound infection, paralytic ileus and bladder atony all of which were conservatively managed with no mortality. At median follow-up of 36 months (range 12-60 months there was no recurrence in patients with stage 1B2 and stage IIA, 25 out of 38 (65.8% were event free and the overall survival was 71%. Conclusion: Many patients in Indian scenario receive suboptimal therapy in locally advanced cervical cancer. EBRT with chemotherapy followed by type 1 extra-fascial hysterectomy can be a good alternative for these patients.

  6. Interobserver variation in rectal and bladder doses in orthogonal film-based treatment planning of cancer of the uterine cervix

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    Raghukumar P

    2008-01-01

    Full Text Available Orthogonal film-based treatment planning is the most commonly adopted standard practice of treatment planning for cancer of the uterine cervix using high dose rate brachytherapy (HDR. This study aims at examining the variation in rectal and bladder doses when the same set of orthogonal films was given to different observers. Five physicists were given 35 pairs of orthogonal films obtained from patients who had undergone HDR brachytherapy. They were given the same instructions and asked to plan the case assuming the tumor was centrally placed, using the treatment-planning system, PLATO BPS V13.2. A statistically significant difference was observed in the average rectal (F = 3.407, P = 0.01 and bladder (F = 3.284, P = 0.013 doses and the volumes enclosed by the 100% isodose curve ( P < 0.01 obtained by each observer. These variations may be attributed to the differences in the reconstruction of applicators, the selection of source positions in ovoids and the intrauterine (IU tube, and the differences in the selection of points especially for the rectum, from lateral radiographs. These variations in planning seen within a department can be avoided if a particular source pattern is followed in the intrauterine tube, unless a specific situation demands a change. Variations in the selection of rectal points can be ruled out if the posterior vaginal surface is clearly seen.

  7. Risk-factors and strategies for control of carcinoma cervix in India: Hospital based cytological screening experience of 35 years

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    J S Misra

    2009-01-01

    Full Text Available Purpose: Role of risk factors in cervical carcinogenesis and strategies for control of the disease have been assessed from the accumulated cytological data, derived from 35 years of hospital-based screening in Lucknow, North India. Materials and Methods: A total of 36,484 women have been cytologically screened during a span of 35 years (April 1971 - June 2005 in the Gynaecology out patient department (OPD of Queen Mary′s Hospital. Results: The frequency of Squamous Intraepithelial Lesion (SIL and carcinoma was found to be 7.2% and 0.6%, respectively, in the present study. The study revealed high age and parity as a predominant factor in cervical carcinogenesis, while viral sexually transmitted disease (STDs -human papilloma virus (HPV and Herpes simplex virus (HSV were also largely associated with SIL cases. The study emphasized great value of clinically downstaging the cervical cancer by detecting cervical cancer in the early stage. The study also revealed a significant difference in the frequency of SIL in symptomatic and asymptomatic women. Conclusion: Based on the analyzed data, it was felt that single lifetime screening, which appears to be the most feasible and affordable mode for control of carcinoma cervix in developing countries like India, should be carried out in all women of high parity irrespective of age (with three or more children and in older women above the age of 40 years irrespective of parity.

  8. Tannic acid binding of cell surfaces in normal, premalignant, and malignant squamous epithelium of the human uterine cervix.

    Science.gov (United States)

    Davina, J H; Lamers, G E; van Haelst, U J; Kenemans, P; Stadhouders, A M

    1984-01-01

    Alterations in tannic acid (TA) binding capacity of cell surface carbohydrates in normal, premalignant, and malignant squamous epithelium of the human uterine cervix have been studied using electron microscopic visualization in combination with microdensitometric evaluation. While in normal epithelium there is distinct binding in four to five cell layers of the deep intermediate zone, cells of carcinoma in situ and invasive cancer lesions lack TA binding. In moderate dysplasia an intermediate reacting pattern is found. Deep intermediate cells in areas bordering the carcinoma in situ lesions do not show any binding, although their ultrastructure cannot be distinguished from similar cells in normal tissue. The TA deposition within the deep intermediate zone is probably related to the presence here of glycoprotein-containing membrane-coating granules. The finding that TA binding discriminates between cells in normal squamous epithelium and morphologically normal cells in juxtaposition with lesional areas in premalignant and malignant epithelium opens the possibility for a more reliable cytologic diagnosis of cervical epithelial neoplasia.

  9. AgarCyto: a novel cell-processing method for multiple molecular diagnostic analyses of the uterine cervix.

    Science.gov (United States)

    Kerstens, H M; Robben, J C; Poddighe, P J; Melchers, W J; Boonstra, H; de Wilde, P C; Macville, M V; Hanselaar, A G

    2000-05-01

    In diagnostic cytology, it has been advocated that molecular techniques will improve cytopathological diagnosis and may predict clinical course. Ancillary molecular techniques, however, can be applied only if a sufficient number of preparations are made from a single cell sample. We have developed the AgarCyto cell block procedure for multiple molecular diagnostic analyses on a single scraping from the uterine cervix. The optimized protocol includes primary fixation and transport in ethanol/carbowax, secondary fixation in Unifix, and embedding in 2% agarose and then in paraffin according to a standard protocol for biopsies. More than 20 microscopic specimens were produced from a single AgarCyto cell block, and standard laboratory protocols have been successfully applied for H&E staining, immunohistochemistry for Ki-67 and p53, and in situ hybridization for the centromere of human chromosome 1 and human papilloma virus Type 16. In addition, single AgarCyto sections yielded sufficient input DNA for specific HPV detection and typing by LiPA-PCR, and the protocol includes an option for DNA image cytometry. The AgarCyto cell block protocol is an excellent tool for inventory studies of diagnostic and potentially prognostic molecular markers of cervical cancer.

  10. Differential role of gene hypermethylation in adenocarcinomas, squamous cell carcinomas and cervical intraepithelial lesions of the uterine cervix.

    Science.gov (United States)

    Blanco-Luquin, Idoia; Guarch, Rosa; Ojer, Amaya; Pérez-Janices, Noemí; Martín-Sánchez, Esperanza; Maria-Ruiz, Sergio; Monreal-Santesteban, Iñaki; Blanco-Fernandez, Laura; Pernaut-Leza, Eduardo; Escors, David; Guerrero-Setas, David

    2015-09-01

    Cervical cancer is the third most common cancer in women worldwide. The hypermethylation of P16, TSLC-1 and TSP-1 genes was analyzed in squamous cell carcinomas (SCC), cervical intraepithelial lesions (CIN) and adenocarcinomas (ADC) of the uterine cervix (total 181 lesions). Additionally human papillomavirus (HPV) type, EPB41L3, RASSF1 and RASSF2 hypermethylation were tested in ADC and the results were compared with those obtained previously by our group in SCC. P16, TSLC-1 and TSP-1 hypermethylation was more frequent in SCCs than in CINs. These percentages and the corresponding ones for EPB41L3, RASSF1 and RASSF2 genes were also higher in SCCs than in ADCs, except for P16. The presence of HPV in ADCs was lower than reported previously in SCC and CIN. Patients with RASSF1A hypermethylation showed significantly longer disease-free survival (P = 0.015) and overall survival periods (P = 0.009) in ADC patients. To our knowledge, this is the first description of the EPB41L3 and RASSF2 hypermethylation in ADCs. These results suggest that the involvement of DNA hypermethylation in cervical cancer varies depending on the histological type, which might contribute to explaining the different prognosis of patients with these types of tumors.

  11. Analysis of the severe complications of irradiation of carcinoma of the uterine cervix: treatment with intracavitary radium and parametrial irradiation

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    Unal, A.; Hamberger, A.D.; Seski, J.C.

    1981-08-01

    Between January 1967 and December 1974, 254 patients with carcinoma of the uterine cervix were treated with either intracavitary radium and parametrial irradiation or 2000 rad whole pelvis irradiation followed by intracavitary radium and parametrial irradiation. In general, these patients had tumors of relatively limited volume and vaginal and uterine anatomy that was favorable for intracavitary radium. Not all patients had a lymphangiogram performed prior to irradiation. Of those who did, only patients with negative lymphangiograms are included in this analysis. All patient had a minimum follow-up of four years. The absolute four year survival rate for this group of patients was 86%. Only 6.3% of patients died of disease. The incidence of severe complications was 7.5%. Complications were associated with a high number of milligram hours of radium plus a high dose of external irradiation, in combination with either asymmetry of the radium system and/or history of previous pelvic inflammatory disease, pelvic surgery, or diverticulosis.

  12. Presentation of therapy scheme and irradiation technique of short-term contact radiotherpy of the carcinoma of the cervix

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    Annweiler, H.; Roth, S.L.; Thesen, N.; Sack, H.

    1985-05-01

    Since April 1983 patients with gynecologic tumors have been irradiated with the HDR afterloading method at the University Hospital of Cologne. The therapy scheme for the carcinoma of the cervix consists of a combination of intracavitary contact irradiation and external radiotherapy. Brachytherapy is preponderant in an early stage of tumor extension, whereas teletherapy contributes more to the total dose in advanced stages. At first, the pelvis is totally exposed to a homogenous irradiation, so the shrunken tumor can more easily be arrived by curietherapy. The therapy scheme is described for the different tumor stages with its dosages, fractionations, and treatment pauses. Besides the use of special multiple-way applicators, the risk organs are protected by collimating with a block the middle part of the external irradiation field as soon as the maximum permissible dose is reached. A special block shape minimizes the dose irregularities at the field borders. The total physical dose at point A is about 60 Gy. The high dose rate of HDR afterloading has to be considered when calculating the biologic efficient dose. Here the dose rate factor furnishes a rough relation to the established radium dosage.

  13. ADP-ribose polymer - a novel and general biomarker of human cancers of head & neck, breast, and cervix

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    Sharan Rajeshwar N

    2010-10-01

    Full Text Available Abstract Background Poly-ADP-ribosylation, a reversible post-translational modification of primarily chromosomal proteins, is involved in various cellular and molecular processes including carcinogenesis. ADP-ribose polymer or poly-ADP-ribose adducts are enzymatically added onto or stripped off the target chromosomal proteins during this metabolic process. Due to this, the chromatin superstructure is reversibly altered, which significantly influences the pattern of gene expression. We hypothesize that a decrease in the concentration of total poly-ADP-ribose adducts of peripheral blood lymphocyte (PBL proteins strongly correlates with the incidence of human cancer. Results Using a novel immunoprobe assay, we show a statistically significant (P ≤ 0.001 reduction (~ 42 to 49% in the level of poly-ADP-ribose adducts of PBL proteins of patients with advanced cancers of head & neck (H & N region (comprising fourteen distinct cancers at different sites, breast and cervix in comparison to healthy controls. Conclusions These findings imply potential utility of the poly-ADP-ribose adducts of PBL proteins as a novel and general biomarker of human cancers with potentials of significant clinical and epidemiological applications.

  14. [Therapeutic interruption of pregnancy in the second trimester after preparation of the cervix with prostaglandins F2 alpha].

    Science.gov (United States)

    Maria, B; Stampf, F; Barrat, J

    1983-01-01

    The use of prostaglandins is one of the best techniques for second trimester abortion. The authors present 40 patients aborted by a new two stage technique: First: ripening of the cervix a tylose gel containing 5 or 10 mg PGF2 alpha every 12 hours during 3 days. Then, if abortion is not obtained, previously published techniques are used: intra-amniotic injection (40 mg PGF2 alpha) or intra-cervical infusion (2 mg/hour). The mean duration of these abortions is 52 hours using 29 mg of PGF2 alpha. 22 patients aborted during ripening (mean = 27 hours), 12 patients needed an intracervical infusion (mean = 88 hours) and 6, an intra-amniotic injection (mean = 72 hours). It seems that abortion is easier when pregnancy is earlier, under 20 weeks of gestation. Few side effects were observed: only three cases of fever with a single case of endometritis. Failure of this technique occurred in five patients (4 aborted outside the time allowed for, and one needed instrumental extraction). With this technique, the mean duration is longer than those previously described in the literature but the efficiency is over 90%.

  15. Identification of pelvic lymph nodes with chlorophyllin after injection into the uterine cervix: an experimental and clinical study.

    Science.gov (United States)

    Wang, H; Tan, Y; Wang, X; Xie, J

    2001-06-01

    We investigated the value of staining retroperitoneal lymph nodes with chlorophyllin in normal dogs and in women with malignant uterine tumors undergoing lymphadenectomy. In dogs, after 0.3% chlorophyllin (sodium copper chlorophyllin) was injected into the canine uterus, the concentration of dye in the bloodstream was measured with a spectrophotometer and sections of stained retroperitoneal lymph nodes were examined using light and electron microscopy. The highest blood levels were detected at 4 hrs and nearly all of the chlorophyllin was gone from the bloodstream by 18 hrs but was retained in nodal macrophages for at least 4 days. No morphological changes were found in the excised lymph nodes. Twenty-four patients with cervical carcinoma and 20 patients with endometrial carcinoma undergoing radical hysterectomy and lymphadenectomy were divided into a lymphatic coloration group (23 patients) and a non-coloration (control) group (21 patients). In the lymphatic coloration group (0.3% chlorophyllin) was injected into the cervix 5 days before elective lymphadenectomy. There were no complications attributed to injection of the chlorophyllin. The number of dissected lymph nodes in the coloration group were greater than the control group (pchlorophyllin is safe and facilitates identification of retroperitoneal lymph nodes, allows more complete nodal excision and shortens the time of operation in patients undergoing radical hysterectomy with lymphadenectomy.

  16. Evaluation of the treatment of autopsied cases of carcinoma of the cervix with reference to surgical operation and radiotherapy

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    Ogawa, Masatoshi; Suzuki, Mitsuaki; Takeda, Hideo; Jobo, Toshiko; Arai, Masao (Kitasato Univ., Sagamihara, Kanagawa (Japan). School of Medicine)

    1982-08-01

    Results of thirty two autopsies in the cases of carcinoma of the cervix experienced in ten years were analyzed. Fourteen cases received surgery and postoperative irradiation. Fourteen cases had external irradiation combined with intracavitary irradiation. Cancer was the cause of death in 81.3% of the whole cases, and it caused cachexia in many cases of the two groups. Uremia and uremic complication were found in 8 cases of the operative group, and in 2 of the radiotherapy group. High incidences of intrapelvic local recurrence and metastasis to other organs were found in the operative group. Cancerous infiltrative involvement of the bladder was often observed, such as in 11 cases of the operated group and in 9 cases of the irradiation group. Cancerous hydronephrosis was found in 10 and 5 cases of the operation and irradiation group, respectively; non-cancerous hydronephrosis was found in 2 of the former and in 4 of the latter. The radiotherapy group had a longer survival period than the other, without severe side effects in the urinary system. This suggested that radiotherapy is of value in treatment of cervical cancer.

  17. Coexpression of neuropeptide Y and vasoactive intestinal polypeptide in pelvic plexus neurones innervating the uterus and cervix in the rat.

    Science.gov (United States)

    Houdeau, E; Boyer, P A; Rousseau, A; Rousseau, J P

    1997-05-01

    The present study investigates the distribution and coexpression of neuropeptide Y (NPY) and vasoactive intestinal peptide (VIP) in neurones of the accessory ganglion (AG), hypogastric plexus (HP) and paracervical ganglion (PCG), which compose the pelvic plexus in the female rat. Nerve cell bodies immunoreactive for NPY and VIP represent 84% and 46% of the neurone population in the PCG, respectively, while immunoreactivity for each peptide is observed in about 90% of the AG and HP neurones. Adjacent sections immunostained for NPY and VIP, as well as the use of immunocytochemistry combined with in situ hybridization show that 92% of the VIP-containing neurones in the pelvic plexus also contain NPY. In addition, a retrograde tracing study performed in combination with immunocytochemistry demonstrates that pelvic plexus neurones project preferentially to the lower part of the uterus and to the cervix, and that about 95% of these projecting neurones contain VIP. Taken together, our findings indicate that in the female rat, neurones of the pelvic plexus projecting to the lower genital tract mainly coexpress VIP and NPY, and supply nerve fibres to the vascular and nonvascular smooth muscle in the uterocervical region. Since NPY and VIP exert distinct effects according to the target tissue, our results suggest that neurones coexpressing these peptides play important roles in the local regulation of the vascular bed and motor activity of the lower genital tract.

  18. Tungsten Oxide Nanoplates; the Novelty in Targeting Metalloproteinase-7 Gene in Both Cervix and Colon Cancer Cells.

    Science.gov (United States)

    Yassin, Abdelrahman M; Elnouby, Mohamed; El-Deeb, Nehal M; Hafez, Elsayed E

    2016-10-01

    In this study, we synthesized tungsten oxide (WO3) nanoplates, both crystallographic phases and the morphology of the samples were determined by powder x-ray diffraction and the scanning electron microscopy, respectively. The obtained data clarified that, the all prepared WO3·H2O samples were composed of large quantity of nanoplates. The cytotoxicity patterns of nanoplates were checked on both normal and cancer mammalian cell lines. Both nanoplates cytotoxicity did not exceed the 50 % inhibitory concentration (IC50) on the all normal tested cells even by using concentrations up to 1 mg/ml. In addition, orthorhombic tungsten oxide nanoplate was more potent against both Caco2 and Hela cells by showing inhibition percentages in cellular viability 64.749 and 72.27, respectively, and with cancer selectivity index reached 3.2 and 2.6 on both colon and cervix cancer, respectively. The anticancer effects of nanoplates were translated to alteration in both pro-apoptotic and anti-apoptotic genes expressions. Tungsten oxide nanoplates down regulated the expression of B cell lymphoma 2 (Bcl-2) and metalloproteinase-7 (MMP7) genes. In addition, orthorhombic tungsten oxide nanoplates showed more potentiation in IL2 and IL8 induction (40.43 pg/ml) and upregulation of TNF-α gene expression but with lower folds than Escherichia coli lipopolysaccharide (LPS) induction.

  19. Properties of Surfactin C-15 Nanopeptide and Its Cytotoxic Effect on Human Cervix Cancer (HeLa Cell Line

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    Zahra Nozhat

    2012-01-01

    Full Text Available Surfactin is one of the most powerful biosurfactants that has been known so far. It is an acidic cyclic nonribosomal lipoheptapeptide that is produced by Bacillus subtilis. In this presentation we investigated different properties of surfactin C-15. The nanomicelle forming ability of surfactin C-15 in different aqueous environments with various ionic strengths was studied by scanning electron microscope. Surfactin second structure was investigated by Far-UV CD spectrum. Its hemolytic activity and cytotoxicity were measured by hemolysis and MTT assays, respectively. Surfactin formed spherical nanomicelles in distilled water and amorphous nanomicelles in PBS buffer . The hemolysis assay results indicated that HC50 of surfactin was 47 μM. Surfactin C-15 arrested growth of human cervix cancer HeLa cell line in a time- and dosage-dependent method, so that its IC50 at 16, 24, and 48h were 86.9, 73.1, and 50.2 μM, respectively.

  20. Osteoclastic Giant Cell Rich Squamous Cell Carcinoma of the Uterine Cervix: A Case Report and Review of the Literature

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    Lucía Alemán-Meza

    2014-01-01

    Full Text Available Cervical carcinoma is the most common malignancy of the female genital tract and represents the second most common malignancy in women worldwide. Histologically 85 to 90% of cervical cancers are squamous cell carcinoma. Osteoclastic giant cell rich squamous cell carcinoma is an unusual histological variant of which only 4 cases have been reported. We present the case of a 49-year-old woman with a 6-month history of irregular vaginal bleeding. Examination revealed a 2.7 cm polypoid mass in the anterior lip of the uterine cervix. The patient underwent hysterectomy with bilateral salpingo-oophorectomy. Microscopically the tumor was composed of infiltrative nests of poorly differentiated nonkeratinizing squamous cell carcinoma. Interspersed in between these tumor cells were numerous osteoclastic giant cells with abundant eosinophilic cytoplasm devoid of nuclear atypia, hyperchromatism, or mitotic activity. Immunohistochemistry was performed; CK and P63 were strongly positive in the squamous component and negative in the osteoclastic giant cells, while CD68 and Vimentin were strongly positive in the giant cell population and negative in the squamous component. The patient received chemo- and radiotherapy for recurrent disease identified 3 months later on a follow-up CT scan; 7 months after the surgical procedure the patient is clinically and radiologically disease-free.

  1. 剖宫产后阴道分娩35例相关因素分析%Splits the Palace Post-natal Vagina Childbirth 35 Example Correlation Factors to Analyze

    Institute of Scientific and Technical Information of China (English)

    邹清如; 蔡芬兰; 黄利兰; 宋薇

    2012-01-01

      目的 探讨剖宫产术后阴道分娩(VBAV)的相关因素.方法 回顾性分析135例瘢痕子宫孕妇的临床资料,根据分娩方式分为阴道分娩组(实验组)35例和剖宫产组(对照组)100例,对孕妇的一般情况进行调查分析,并在两组之间进行对比.结果 两组产妇在阴道分娩史、前次剖宫产指征、胎龄、新生儿体质量、宫颈条件等方面有统计学差异(P0.05).结论 VBAV与多种因素有关,针对上述相关因素可适时选择阴道分娩,减少剖宫产率,提高产科质量.%  Objective After the goal discussion splits the palace to produce the technique the vagina childbirth (VBAV) correlation factor. Method Review analysis 135example scar womb pregnant woman's clinical material, divides into the vagina childbirth group according to the childbirth way (experimental group) 35 examples and splits the palace to produce the group (control group) 100 examples, carries on the diagnosis to pregnant woman's ordinary circumstances, and carries on the contrast between two groups. Result The two group of parturient women in the vagina childbirth history, recently split the palace to produce refer draft, aspects and so on embryo age, newborn body weight, cervix of the uterus condition have statistics difference (P0.05).Conclusion VBAV and many kinds of factors related, may at the right moment choose the vagina childbirth in view of the above correlation factor, reduces splits the palace production rate, improves obstetrics quality.

  2. Carcinoma of the uterine cervix: a review of its pathology and commentary on the problem in Malaysians.

    Science.gov (United States)

    Cheah, P L; Looi, L M

    1999-06-01

    Since its recognition about 150 years ago, there has been much progress in the understanding of the pathogenesis, prevention, early detection and management of carcinoma of the uterine cervix. Important historical landmarks include the (1) recognition of pre-invasive and pre-clinical lesions, and the devise of various systems for reporting these lesions, (2) improvements in diagnostic techniques particularly colposcopy, (3) advent of therapeutic procedures (electrocoagulation, cryotherapy, laser therapy and loop electrosurgical excision), and (4) recognition of the aetiological relationship between the human papillomavirus and cervical neoplasia. The susceptibility of the cervical transformation zone to malignant change is now well recognised. The WHO classification system remains the one most commonly utilised for histological reporting of cervical cancers. In the recent 1994 update, cervical carcinoma is divided into 3 main categories: squamous cell carcinoma, adenocarcinoma and other epithelial tumours. Squamous cell carcinoma (60-80%) predominates among invasive cervical carcinoma. Recognised variants include verrucous, warty (condylomatous), papillary squamous (transitional) and lymphoepithelioma-like carcinoma. Adenocarcinoma (5-15% of invasive carcinomas) shows an increasing trend in young females. Like its squamous counterpart, preinvasive and microinvasive versions are known. Variants such as mucinous, endometrioid, clear cell, mesonephric, serous, villoglandular and minimal deviation carcinoma are now defined. Adenosquamous carcinoma (5-25%), adenoid-cystic, adenoid-basal, neuroendocrine and undifferentiated carcinomas constitute other epithelial tumours of the cervix. The management of invasive cervical carcinoma remains heavily dependent on its stage. The FIGO staging system remains the most widely used. The 1995 update provides more definite criteria in subdividing stage IA tumours by delimiting stromal invasion of stage IA1 lesions to a maximum depth

  3. Dual Tracer PET Imaging with FDG and FLT Differentiates Tuberculous Lymphadenopathy from Metastases in a Case of Carcinoma Cervix

    Energy Technology Data Exchange (ETDEWEB)

    Joshi, Prathamesh; Lele, Vikram; Aland, Parag; Gemawat, Shilpa [Jaslok Hospital and Research Centre, Woril (India)

    2013-09-15

    A forty-year-old woman with a known case of carcinoma cervix underwent 18-fluorodeoxyglucose positron emission tomography.computed tomography (18-FDG PET/CT) for evaluation of abdominal lymphadenopathy. Her treatment history included radical hysterectomy and radiotherapy 6 months ago. She complained of weight loss of 7 kg over last 5 months. The maximum intensity projection (MIP) image (Fig. 1a, arrows) revealed multiple areas of intense FDG uptake, which on CT and fused PET/CT images were localized to multiple lymph nodes in bilateral cervical region, right axilla, mediastinum and abdomen. The SUVmax of right axillary lymph nodes (most FDG avid of all lymph node groups) was 15.3. There was no evidence of metabolically active disease or CT demonstrable abnormality in rest of the body. Presence of metabolically active disease in extensive supradiaphramatic lymphadenopathy was unusual for a case of carcinoma cervix. This finding, along with history of significant weight loss and absence of extranodal disease, was suspicious for unrelated pathology like lymphoproliferative disorder or granulomatous disease. Mixed malignant and benign lymphadenopathy was also considered a possibility. To solve the conundrum, 18-fluoro-L-thymidine (FLT) PET/CT was performed on the next day. This scan was performed to assess the proliferation rate in various above-mentioned lymph nodes, and to plan the optimum site of biopsy. The FLT PET scan (Fig. 1b) showed physiological distribution of the tracer in bone marrow, liver, gall bladder and urinary bladder. There was minimal FLT uptake in the enlarged, FDG avid lymph nodes (Fig. 1c and d). SUVmax of FLT uptake in right axillary lymph nodes was 1.4 (SUVmax of FDG uptake = 15.8). The SUVmax of FLT uptake in cervical, mediastinal and abdominal lymph nodes were 1.3, 1.4 and 1.0 respectively. (SUVmax of FDG uptake 12.1, 12.7 and 11.9, respectively). Considering avidity for FDG and non-avidity of proliferation marker tracer (FLT), possibility of

  4. Clinical Behaviors and Outcomes for Adenocarcinoma or Adenosquamous Carcinoma of Cervix Treated by Radical Hysterectomy and Adjuvant Radiotherapy or Chemoradiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Yi-Ting; Wang, Chun-Chieh; Tsai, Chien-Sheng [Department of Radiation Oncology, Chang Gung Memorial Hospital, Lin-Kou, Chang Gung University, Taoyuan, Taiwan (China); Department of Medical Imaging and Radiological Science, Chang Gung University, Taoyuan, Taiwan (China); Lai, Chyong-Huey; Chang, Ting-Chang; Chou, Hung-Hsueh [Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Lin-Kou, Chang Gung University, Taoyuan, Taiwan (China); Lee, Steve P. [Department of Radiation Oncology, University of California, Los Angeles School of Medicine, Los Angeles, CA (United States); Hong, Ji-Hong, E-mail: jihong@adm.cgmh.org.tw [Department of Radiation Oncology, Chang Gung Memorial Hospital, Lin-Kou, Chang Gung University, Taoyuan, Taiwan (China); Department of Medical Imaging and Radiological Science, Chang Gung University, Taoyuan, Taiwan (China)

    2012-10-01

    Purpose: To compare clinical behaviors and treatment outcomes between patients with squamous cell carcinoma (SCC) and adenocarcinoma/adenosquamous carcinoma (AC/ASC) of the cervix treated with radical hysterectomy (RH) and adjuvant radiotherapy (RT) or concurrent chemoradiotherapy (CCRT). Methods and Materials: A total of 318 Stage IB-IIB cervical cancer patients, 202 (63.5%) with SCC and 116 (36.5%) with AC/ASC, treated by RH and adjuvant RT/CCRT, were included. The indications for RT/CCRT were deep stromal invasion, positive resection margin, parametrial invasion, or lymph node (LN) metastasis. Postoperative CCRT was administered in 65 SCC patients (32%) and 80 AC/ASC patients (69%). Patients with presence of parametrial invasion or LN metastasis were stratified into a high-risk group, and the rest into an intermediate-risk group. The patterns of failure and factors influencing survival were evaluated. Results: The treatment failed in 39 SCC patients (19.3%) and 39 AC/ASC patients (33.6%). The 5-year relapse-free survival rates for SCC and AC/ASC patients were 83.4% and 66.5%, respectively (p = 0.000). Distant metastasis was the major failure pattern in both groups. After multivariate analysis, prognostic factors for local recurrence included younger age, parametrial invasion, AC/ASC histology, and positive resection margin; for distant recurrence they included parametrial invasion, LN metastasis, and AC/ASC histology. Compared with SCC patients, those with AC/ASC had higher local relapse rates for the intermediate-risk group but a higher distant metastasis rate for the high-risk group. Postoperative CCRT tended to improve survival for intermediate-risk but not for high-risk AC/ASC patients. Conclusions: Adenocarcinoma/adenosquamous carcinoma is an independent prognostic factor for cervical cancer patients treated by RH and postoperative RT. Concurrent chemoradiotherapy could improve survival for intermediate-risk, but not necessarily high-risk, AC/ASC patients.

  5. Clinical significance of cumulative biological effective dose and overall treatment time in the treatment of carcinoma cervix

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    Mandal Abhijit

    2007-01-01

    Full Text Available The purpose of this retrospective study is to report the radiotherapy treatment response of, and complications in, patients with cervical cancer on the basis of cumulative biologic effective dose (BED and overall treatment time (OTT. Sixty-four (stage II - 35/64; stage III - 29/64 patients of cervical cancer were treated with combination of external beam radiotherapy (EBRT and low dose rate intracavitary brachytherapy (ICBT. The cumulative BED was calculated at Point A (BED 10 ; and bladder, rectal reference points (BED 2.5 using the linear-quadratic BED equations. The local control (LC rate and 5-year disease-free survival (DFS rate in patients of stage II were comparable for BED 10 < 84.5 and BED 10 > 84.5 but were much higher for BED 10 > 84.5 than BED 10 < 84.5 ( P < 0.01 in stage III patients. In the stage II patients, The LC rate and 5-year DFS rate were comparable for OTT < 50 days and for OTT> 50 days but were much higher in stage III patients with OTT < 50 than OTT> 50 days ( P < 0.001. It was also observed that patients who received BED 2.5 < 105 had lesser rectal ( P < 0.001 and bladder complications than BED 2.5 > 105. Higher rectal complication-free survival (CFS R rate, bladder complication-free survival (CFS B rate and all-type late complication-free survival rate were observed in patients who received BED 2.5 < 105 than BED 2.5 > 105. A balanced, optimal and justified radiotherapy treatment schedule to deliver higher BED 10 (>84.5 and lower BED 2.5 (< 105 in lesser OTT (< 50 days is essential in carcinoma cervix to expect a better treatment outcome in all respects.

  6. Clinical significance of geographic miss when using conventional four field radiotherapy technique in treatment of locally advanced carcinoma cervix

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    A Gulia

    2016-01-01

    Full Text Available Background: Although conventional four- field radiotherapy based on bony landmarks has been traditionally used, areas of geographical miss due to individual variation in pelvic anatomy have been identified with advanced imaging techniques. AIMS: The primary aim of this study is to evaluate the geographical miss in patientswhen using the conventional four-field planningplanning and to find out the impact of 3-D conformal CT based in patients with locally advanced carcinoma cervix.Materials and Methods: In 50 patients, target volume delineation was done on planning computed tomography (CT scans, according to guidelines by Taylor et al. Patients were treated with modified four field plan, except for the superior, where field border was kept at L4-L5 interspace A dosimetric comparison was done between the conventional four-field based on bony landmarks and the target volume delineated on computed tomography. The disease free survival, pelvic and para aortic nodal free survival, distant failures free survival were calculated using Kaplan Meir Product Limit Method. Results: Patients were followed-up for a median period of 11 months. The median V95 for conventional and modified extended four field plans were 89.4% and 91.3% respectively. Patients with V95 for modified extended pelvic fields less than 91.3% had a trend toward inferior disease free survival (mean DFS 9.8 vs. 13.9 months though the difference was not statistically significant log rank test.Conclusions: Our preliminary data shows trend toward lower DFS in patients with inadequate target volume coverage. We recommend routine use of CT based planning for four field technique.

  7. Genomic profiling identifies common HPV-associated chromosomal alterations in squamous cell carcinomas of cervix and head and neck

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    Leemans C René

    2009-06-01

    Full Text Available Abstract Background It is well known that a persistent infection with high-risk human papillomavirus (hrHPV is causally involved in the development of squamous cell carcinomas of the uterine cervix (CxSCCs and a subset of SCCs of the head and neck (HNSCCs. The latter differ from hrHPV-negative HNSCCs at the clinical and molecular level. Methods To determine whether hrHPV-associated SCCs arising from different organs have specific chromosomal alterations in common, we compared genome-wide chromosomal profiles of 10 CxSCCs (all hrHPV-positive with 12 hrHPV-positive HNSCCs and 30 hrHPV-negative HNSCCs. Potential organ-specific alterations and alterations shared by SCCs in general were investigated as well. Results Unsupervised hierarchical clustering resulted in one mainly hrHPV-positive and one mainly hrHPV-negative cluster. Interestingly, loss at 13q and gain at 20q were frequent in HPV-positive carcinomas of both origins, but uncommon in hrHPV-negative HNSCCs, indicating that these alterations are associated with hrHPV-mediated carcinogenesis. Within the group of hrHPV-positive carcinomas, HNSCCs more frequently showed gains of multiple regions at 8q whereas CxSCCs more often showed loss at 17p. Finally, gains at 3q24-29 and losses at 11q22.3-25 were frequent (>50% in all sample groups. Conclusion In this study hrHPV-specific, organ-specific, and pan-SCC chromosomal alterations were identified. The existence of hrHPV-specific alterations in SCCs of different anatomical origin, suggests that these alterations are crucial for hrHPV-mediated carcinogenesis.

  8. Protein p 16INK4A expression in cervical intraepithelial neoplasia and invasive squamous cell carcinoma of uterine cervix

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    Gupta Ruchi

    2010-01-01

    Full Text Available The association of human papilloma virus (HPV infection and cervical intraepithelial neoplasia (CIN is well recognized. Interaction of HPV oncogenic proteins with cellular regulatory proteins leads to up regulation of p16 INK4A , a CDK inhibitor, which is a biomarker for HPV infection. We investigated p16 expression in CIN and invasive squamous cell carcinoma (SCC which has not been reported in the Indian population previously. Materials and Methods: Retrospective analysis of 100 cases with 20 cases each of histologically normal cervical epithelium, CIN1, 2, 3 and invasive SCC for p16 expression was performed by immunohistochemistry using commercially available mouse monoclonal antibody to p16 (clone 6H12. Statistical Analysis: For differences in expression among groups, statistical analysis was carried out using ANOVA and post hoc test of Scheffe. Results: p16 immunoreactivity was found to be both nuclear and/or cytoplasmic. The normal cervical epithelium was predominantly negative for p16 (18/20. There was a progressive increase of p16 expression with the grade of CIN. In CIN 1, two cases (20% showed nuclear and nucleocytoplasmic positivity respectively. In contrast, diffuse strong nuclear or nucleocytoplasmic expression was observed in 45 and 55% cases of CIN 2 and CIN 3 respectively. All except one squamous cell carcinoma stained strongly positive for p16. The difference in expression between CIN 2/3 and SCC versus normal cervix was found highly significant (p is equal to 0.008 and p less than 0.001. Conclusions: p16 expression correlates excellently with the grade of CIN and is a sensitive marker of cervical intraepithelial neoplasia.

  9. The Cervix Cancer Research Network (CCRN: Increasing access to cancer clinical trials in low- and middle-income countries

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    Gita eSuneja

    2015-02-01

    Full Text Available Introduction: The burden of cervical cancer is large and growing in developing countries, due in large part to limited access to screening services and lack of human papillomavirus (HPV vaccination. In spite of modern advances in diagnostic and therapeutic modalities, outcomes from cervical cancer have not markedly improved in recent years. Novel clinical trials are urgently needed to improve outcomes from cervical cancer worldwide. Methods: The Cervix Cancer Research Network (CCRN, a subsidiary of the Gynecologic Cancer InterGroup (GCIG, is a multi-national, multi-institutional consortium of physicians and scientists focused on improving cervical cancer outcomes worldwide by making cancer clinical trials available in low-, middle-, and high-income countries. Standard operating procedures for participation in CCRN include a pre-qualifying questionnaire to evaluate clinical activities and research infrastructure, followed by a site visit. Once a site is approved, they may choose to participate in one of four currently accruing clinical trials.Results: To date, 13 different CCRN site visits have been performed. Of these 13 sites visited, 10 have been approved as CCRN sites including Tata Memorial Hospital, India; Bangalore, India; Trivandrum, India; Ramathibodi, Thailand; Siriaj, Thailand; Pramongkutklao, Thailand; Ho Chi Minh, Vietnam; Blokhin Russian Cancer Research Center; the Hertzen Moscow Cancer Research Institute; and the Russian Scientific Center of Roentgenoradiology. The four currently accruing clinical trials are TACO, OUTBACK, INTERLACE, and SHAPE.Discussion: The CCRN has successfully enrolled 10 sites in developing countries to participate in four randomized clinical trials. The primary objectives are to provide novel therapeutics to regions with the greatest need and to improve the validity and generalizability of clinical trial results by enrolling a diverse sample of patients.

  10. A prospective randomized controlled trial to study the role of sulfasalazine in prevention of acute gastrointestinal toxicity associated with concurrent chemoradiation in carcinoma cervix

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    Santanu Pal

    2013-01-01

    Full Text Available Background: The primary aim of the study was to evaluate the effectiveness of sulfasalazine in reducing the incidence of acute radiation-induced enteritis in carcinoma cervix patients receiving pelvic external beam radiotherapy along with concurrent cisplatin-based chemotherapy. Materials and Methods: Between November 2011 and July 2012 a total of 98 patients of locoregionally advanced carcinoma of cervix (49 each in study and control arms were enrolled in this study. Patients in both the arms were treated with whole pelvis external beam radiotherapy with total dose of 50 Gy in conventional fractionation. Along with this inj. cisplatin was given concurrently at the dose of 40 mg/m 2 of body surface area every week during radiation for 5 weeks. Concurrent chemoradiation was followed by brachytherapy after a gap of 2 weeks. Patients in the study arm also received tablet sulfasalazine 1,000 mg orally twice daily from the day of starting of radiotherapy to 1 week after completion of treatment. Weekly follow-up of all patients to assess acute toxicities was done using common toxicity criteria version 4.0 (CTC v4.0 toxicity scores. Data analysis was carried out by SPSS version 20.0 software. Results: Incidence of grade II or higher grade, lower gastrointestinal toxicity was 19.14% (09/47 in study arm and 41.66% (20/48 in control arm which was statistically significant (P = 0.017. Conclusion: The study shows that sulfasalazine can significantly reduce the acute radiation-induced diarrhea (ARID in patients undergoing whole pelvis external beam radiotherapy for carcinoma cervix. The drug is safe, cheap, and readily available.

  11. Comparison of molecular signatures in large scale protein interaction networks in normal and cancer conditions of brain, cervix, lung, ovary and prostate

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    Rajat Suvra Banik

    2016-04-01

    Full Text Available Background Cancer, the disease of intricateness, has remained beyond our complete perception so far. Network systems biology (termed NSB is one of the most recent approaches to understand the unsolved problems of cancer development. From this perspective, differential protein networks (PINs have been developed based on the expression and interaction data of brain, cervix, lung, ovary and prostate for normal and cancer conditions. Methods Differential expression database GeneHub-GEPIS and interaction database STRING were applied for primary data retrieval. Cytoscape platform and related plugins named network analyzer; MCODE and ModuLand were used for visualization of complex networks and subsequent analysis. Results Significant differences were observedamong different common network parameters between normal and cancer states. Moreover, molecular complex numbers and overlapping modularization found to be varying significantly between normal and cancerous tissues. The number of the ranked molecular complex and the nodes involved in the overlapping modules were meaningfully higher in cancer condition.We identified79 commonly up regulated and 6 down regulated proteins in all five tissues. Number of nodes, edges; multi edge node pair, and average number of neighbor are found with significant fluctuations in case of cervix and ovarian tissues.Cluster analysis showed that the association of Myc and Cdk4 proteins is very close with other proteins within the network.Cervix and ovarian tissue showed higher increment of the molecular complex number and overlapping module network during cancer in comparison to normal state. Conclusions The differential molecular signatures identified from the work can be studied further to understand the cancer signaling process, and potential therapeutic and detection approach. [Biomed Res Ther 2016; 3(4.000: 605-615

  12. A Community Based Study On The Prevalence Of Risk Factors OF Cancer Cervix In Married Women Of A Rural Area Of West Bengal

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    Dasgupta Aparajita

    2002-01-01

    Full Text Available Research question: What is the prevalence of the established risk factors associated with cancer cervix? Objective: To assess the presence of some determinants of cancer cervix among married, rural women of reproductive age group. Study design: Community based, cross â€" sectional, observational study. Setting: Rural: Village East Gobindopur is Singur block in Hooghly district of West Bengal. Study population : All the married women of reproductive age group (103 of the village were included in the study. Results: More than two â€" third (72.8% of the study population belonged to the vulnerable age group (25-45 years for this disease while 59.3% were married before they attained 18 years of age. Again 50% (approx of the married women gave birth to their first child before they were 18 years of age. One â€" third of the study population had parity higher than three. Two â€" third of the women studied were using one or more methods of family planning and one â€" third of the same population were permanently sterilized, 20% were using oral contraceptive pills and only 7.7% were using barrier methods of contraception. Only 31% of the women had satisfactory genital hygiene practice and 36.9% had symptoms of reproductive tract infection. Conclusion: There was a high prevalence of some important risk factors associated with cancer cervix like age, age of marriage, age of first child birth, parity ,family planning practices, genital hygiene and reproductive tract infections in the study population. Therefore screening and early detection efforts can be directed specifically to the group at risk.

  13. Involvement of the pelvic plexus and the suprarenal ganglia in the neuropeptide Y (NPY) innervation of the cervix and the uterus of the rat.

    Science.gov (United States)

    Serghini, R; Prud'homme, M J; Vaudry, H; Rousseau, J P

    1997-12-03

    The involvement of the pelvic plexus and suprarenal ganglia in the neuropeptide Y (NPY) innervation of the genital tract was studied in the female rat by means of denervation experiments and retrograde tracing studies. Removal of the paracervical ganglia caused a significant decrease of the NPY-immunoreactive nerve density and NPY concentration in the lower part of the genital tract: cervix, uterine body and lower part of the uterine horn. The decrease in NPY concentration in these three regions was more pronounced after lesion of the pelvic plexus. Lesion of the ovarian nerve plexus caused a depletion in the NPY-immunoreactive nerve fibres and a decrease in NPY concentration in the upper part of the uterine horn. Pelvic nerve section, inferior mesenteric ganglia excision and superior ovarian nerve section had no effect on the NPY innervation in the genital tract. Injection of fluorogold into the cervix and lower part of the uterus combined with immunohistochemistry revealed that 87.5% of labelled neurons in the pelvic plexus were NPY-immunoreactive. Following injection of fluorogold into the upper part of the uterus, 92% of labelled neurons in the suprarenal ganglia were NPY-immunoreactive. Treatment with 6-hydroxydopamine revealed that the NPY-immunoreactive nerve fibres were non-noradrenergic in the cervix, but were noradrenergic in the upper part of the uterus. In the uterine body and lower part of the uterine horn, both noradrenergic and non-noradrenergic NPY-immunoreactive nerve fibres were observed. These data demonstrate the major contribution of pelvic plexus neurons in the non-noradrenergic NPY innervation of the lower part of the genital tract, and the involvement of the suprarenal ganglia in the noradrenergic NPY innervation of the upper part of the uterus via the ovarian nerve plexus.

  14. Myeloid Sarcoma of the Uterine Cervix as Presentation of Acute Myeloid Leukaemia after Treatment with Low-Dose Radioiodine for Thyroid Cancer: A Case Report and Review of the Literature

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    Anne Sophie Weingertner

    2009-01-01

    Full Text Available The development of acute myeloid leukaemia after low-dose radioiodine therapy and its presentation as a myeloid sarcoma of the uterine cervix are both rare events. We report a case of acute myeloid leukaemia revealed by a myeloid sarcoma of the uterine cervix in a 48-year-old woman, 17 months after receiving a total dose of 100 mCi 131I for papillary thyroid cancer. A strict hematological follow-up of patients treated with any dose of 131I is recommended to accurately detect any hematological complications which might have been underestimated. Unusual presentations, such as chloroma of the uterine cervix, may reveal myeloid malignancy and should be kept in mind.

  15. Metástase em couro cabeludo de câncer do colo uterino: relato de caso Scalp metastasis from carcinoma of the cervix: case report

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    Sabas Carlos Vieira

    2003-09-01

    Full Text Available Carcinoma de colo uterino é neoplasia comum, porém a ocorrência de metástase cutânea em câncer do colo uterino é rara, variando de 0,1 a 2,0%. Os sítios primários comuns em pacientes com metástase cutânea são mama, pulmão, intestino grosso e ovário. O intervalo entre o diagnóstico do câncer cervical e as lesões metastáticas varia indo desde a apresentação simultânea com a lesão inicial até 5 anos após o tratamento apresentando-se como nódulos em 86,7% das vezes. Representa manifestação de doença avançada e de mau prognostico. Apresentamos um caso de metástase cutânea de câncer de colo uterino em couro cabeludo. A paciente, 43 anos, tinha diagnóstico de carcinoma epidermóide indiferenciado do colo uterino. Evoluiu, seis meses após a cirurgia radical, com recidiva vaginal, sendo tratada com radioterapia pélvica. Quatro meses depois apresentou três nódulos metastáticos indolores em couro cabeludo. A paciente submeteu-se à quimioterapia com regressão completa das lesões do couro cabeludo.Carcinoma of the uterine cervix is a common neoplasm; however skin metastasis from carcinoma of the uterine cervix is a very rare occurrence, varying from 0.1% to 2%. The common primary sites in patients with skin metastasis are the breast, lung, large intestine and ovary. The interval between the diagnosis of cervical cancer and skin lesions ranges from 0 to 69 months, and they present as nodules in 86,7%. Skin metastasis represents a late manifestation of advanced disease with poor prognosis. We present a case of scalp metastasis from carcinoma of the uterine cervix . The patient was 43 years old, had a diagnosis of undifferentiated epidermoid carcinoma of the uterine cervix. Six months after radical surgery she presented with vaginal recurrence, being treated with pelvic radiotherapy. Four months later three painless metastatic nodules appeared at the scalp. The patient underwent chemotherapy with total regression of the

  16. The role of a novel protein ligand hMSH 2 in Vγ9δ2 T cell-mediated anti-cervix cancer immunity

    Institute of Scientific and Technical Information of China (English)

    代玉梅

    2014-01-01

    Objective To explore the role of hMSH2,a novel endogenous tumor-associated protein ligand recognized by Vγ9δ2 T cells,in innate anti-cervix cancer immunity.Methods hMSH2 that expressed on the surface of cervical cancer cell line HeLa cells was blocked by specific antibody.Then the differences in their effects on Vγ9δ2T cells before and after antibody blockage were evaluated by cytotoxicity of Vγ9δ2 T cells and cytokines secretion.

  17. The prognostic value of pimonidazole and tumour pO2 in human cervix carcinomas after radiation therapy: a prospective international multi-center study

    DEFF Research Database (Denmark)

    Nordsmark, Marianne; Loncaster, Julie; Aquino-Parsons, Christina

    2006-01-01

    BACKGROUND AND PURPOSE: Hypoxia adversely affects treatment outcome in human uterine cervical cancer. Here, we present the results of a prospective international multi-centre study evaluating the prognostic value of pre-treatment tumour oxygen partial pressure (pO(2)) and the hypoxia marker...... pimonidazole (pimo). MATERIALS AND METHODS: One hundred and twenty-seven patients with primary cervix cancer were entered. Pre-treatment tumour pO(2) measurements were obtained, and reported by the median tumour pO(2), the fraction of pO(2) values...

  18. Evaluation of radiation doses on critical organs in the treatment of cancer of the cervix using HDR-brachytherapy; Avaliacao das doses em orgaos criticos no tratamento do cancer de colo uterino com braquiterapia de alta taxa de dose

    Energy Technology Data Exchange (ETDEWEB)

    Soares, Taciana; Jansem, Teresa [Pernambuco Univ., Recife, PE (Brazil). Dept. de Biofisica e Radiobiologia; Amaral, Ademir [Pernambuco Univ., Recife, PE (Brazil). Dept. de Energia Nuclear; Cavalcanti, Homero; Vicente, Marcos [Centro de Radioterapia de Pernambuco (CERAPE), Recife, PE (Brazil)

    2000-07-01

    High dose-rate (HDR) brachytherapy is one type of treatment of the cervix carcinoma. During the planning for this therapy, especial attention is given to proximal normal organs such as bladder and rectum. In fact, due to their radiosensibility and localization, bladder and rectum are considered as critical organs. In this work we have studied the influence of the positioning of patient legs in the dose delivered to these critical organs in the treatment of cancer of the cervix using HDR-brachytherapy. (author)

  19. Chemotherapy, brachytherapy and surgery of locally evolved uterine cervix carcinomas: prognosis factors of local control and global survival; Chimioradiotherapie, curietherapie et chirurgie des cancers du col uterin localement evolues: facteurs pronostiques de controle local et de survie globale

    Energy Technology Data Exchange (ETDEWEB)

    Laude, C.; Montella, A.; Montbarbon, X.; Malet, C.; Racadot, S.; Pommier, P. [Centre Leon-Berard, 69 - Lyon (France); Mathevet, P. [Hopital Femme-Mere-Enfant, Hospices Civils de Lyon, 69 - Lyon (France); Buenerd, A. [Centre de Pathologie Est, Hospices Civils de Lyon, 69 - Lyon (France)

    2009-10-15

    The protocol used allows an excellent local control of the uterine cervix carcinoma with an acceptable morbidity. To anticipate the presence of a tumor residue can be an evolution in the therapy management after external radiotherapy, particularly in optimized image-guided brachytherapy (MRI and PET)New utero vaginal applicators with parameters implantation allow to realise the dose complement at the distal parameters. These advances make consider an improvement of results in the management of locally evolved uterine cervix carcinomas. (N.C.)

  20. Vulvovaginal candidiasis during pregnancy and the change of local immune condition of vagina%妊娠期外阴阴道假丝酵母菌病与阴道局部免疫环境变化

    Institute of Scientific and Technical Information of China (English)

    崔颖; 王少为

    2009-01-01

    Objective To examine the local immune condition of vagina, and to investigate the immunological pathogenesis of vulvovnginal candidiasis during pregnancy. Methods Women who visited Beijing Hospital for prenatal care or for health examination during September 2005 to August 2006 were cho-sen and divided into three groups: pregnant women with vtdvovaginal candidiasis (pregnant VVC group, 50 cases), non-pregnant women with VVC (non-pregnant VVC group, 55 cases), healthy women (control group, 35 cases). After primary microscopical screening, vaginal lavnge was centrifuged to separate super-natant, lymphocyte and epithelium. Concentration of IgE was messured by ELISA, and surface immunologi-cal markers of CD4 and CD8 were detected by flow cytometer. Results IgE concentration of the pregnant WC group was lower than that of the non-pregnant WC group, but higher than that of the control group (all Ps <0.05). CD4+ cells and CD4/CD8 ratio for the pregnant WC group were lower than those of the con-trol group (P<0.05). However, CD8 + cells for the pregnant VVC group was higher than that of the con-trol group (P<0.05). Conclusion The changes in immune environment of vagina may play a role in the pathogenesis of pregnant vulvovaginal candidiasis.%目的 了解妊娠期妇女阴道局部免疫特点,探讨妊娠期外阴阴道假丝酵母菌病的免疫学发病机理. 方法 选取2005年9月至2006年8月在卫生部北京医院妇产科门诊就诊及进行健康体检的育龄妇女,分为妊娠外阴阴道假丝酵母菌病组(妊娠VVC,50例)、单纯外阴阴道假丝酵母菌病组(单纯VVC,55例)、正常对照组(35例),经显微镜镜检初步筛选,留取阴道灌洗液,离心后分离上清液、淋巴细胞和上皮细胞成分.用ELISA法测定IgE浓度、流式细胞仪检测淋巴细胞表面免疫标志CD4、CD 8. 结果 IgE水平妊娠VVC组为(1.903±0.274)g/L,单纯VVC组为(2.032±0.289)g/L,正常对照组为(1.347±0.287)g/L,妊娠VVC组与单纯WC组和

  1. ROLE OF COLPOSCOPY USING MODIFIED REID’S INDEX IN SCREENING OF CERVICAL CANCER IN WOMEN WITH ABNORMAL CERVIX ON NAKED EYE EXAMINATION

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    Deshpande

    2014-01-01

    Full Text Available OBJECTIVE: To assess the validity of Colposcopy using Modified Reid's Index as a screening tool in cervical cancer in women with abnormal cervix on naked eye examination . METHODS: This observational study was carried out in Government medical college, Aurangabad from June 2011 to May 2013 . Total 392 women with abnormal cervix on naked eye examination of underwent colposcopy and diagnosis was made on the basis of Modified R eid’s Index. Colposcope directed biopsies were obtained from the abnormal areas. In women with normal colposcopic findings four quadrant biopsies from squamo - columnar junction were taken .Eight women were excluded from analysis owing to unsatisfactory colp oscopy. Results of colposcopy were validated by calculating sensitivity, specificity, positive predictive value, negative predictive value in diagnosing histopathologically confirmed lesions which served as gold standard. RESULTS: Overall sensitivity of colposcopy in all grades of lesions is around 90% or more, touching 100% in high order of histological lesions.Overall specificity is also high.It does have an excellent negative predictive value but comparatively lower positive predictive value especially in high order lesions with fair degree of accuracy in all grades of lesions in the hands of an experienced operator. CONCLUSION: Colposcopy using Modified Reid’s Index with high sensitivity and specificity is a good screening tool for cervical cancer

  2. Losses of expression of the antigens A, Lea and Lex and over-expression of Ley in carcinomas and HG-SIL of the uterine cervix

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    Álvarez-Fernández Emilio

    2008-09-01

    Full Text Available Abstract Background The glycosylation of a great number of molecules, glyco-protein or glycolipids, has been of interest for decades. Objective To compare the expressive patterns of the isoantigenic determinants of histo-blood groups ABH and Lewis in squamous and simple epithelium and in precursors and cancers of the cervix. Methods A total of 36 lesions and neoplasms (10 LG-SIL, 16 HG-SIL and 10 invasive carcinomas have been studied with immunohistochemical techniques, using monoclonal antibodies (MoAb BG1 to BG8 for precursor chains, blood-group ABH and Lewis group Lea, Leb, Lex, and Ley, and four types of lectins. In addition, we have studied the expression of p53 protein and PCNA, establishing the rate of proliferation of each lesion. Using PCR techniques, we have also detected part of the intron of the E6 gene of HPV-16. Results In the invasive cervical carcinomas, we observed a loss of expression of the Lex antigen (p y antigens. With PCNA, we established a proliferative rate which tended to be greater in relation to the progression of the cervix neoplasms. Conclusion These results indicate that there is a relation between the losses of histo-blood groups and the progression of the squamous intraepithelial lesions.

  3. The Effect of Education on the Early Diagnosis of Breast and Cervix Cancer on the Women's Attitudes and Behaviors Regarding Participating in Screening Programs.

    Science.gov (United States)

    Kocaöz, Semra; Özçelik, Hanife; Talas, Melek Serpil; Akkaya, Fulya; Özkul, Fatma; Kurtuluş, Ayla; Ünlü, Fahriye

    2017-03-11

    The objective of this paper was to define the effect of education on the early diagnosis of breast and cervix cancer on the women's attitudes and behaviors regarding participating in Cancer Early Diagnosis, Screening and Training Centers-CEDSTC screening programs. This semi-experimental study was completed with 342 women. The data were collected with forms "Champion's Health Belief Model Scale Breast Cancer-HBMSBC" and "Health Belief Model Scale for Cervical Cancer and the Pap Smear Test-HBMSCCPST." When the women's health beliefs before and after 6 months of the education about the early diagnosis of breast and cervical cancers are considered, it is seen that the HBMSBC subscales health motivation, breast self-examination (BSE), and evasion to mammography (MMG) decreased and BSE self-efficacy and MMG benefit attitudes increased and HBMSCCPST subscales pap smear benefit attitudes increased and evasion to pap smear attitude decreased (p < 0.05). Six months after the education, 28.4% of the women had undergone MMG, 69.9% had performed BSE, and 33.6% had undergone a pap smear test. Education regarding early diagnosis of breast and cervix cancer was found to have positive effects on the health behaviors of the women related to BSE, MMG, and pap smear tests. The women require professional education program for increasing their attitudes and behaviors for CEDSTC screening programs. We suggest regularly providing education to increase participation in early screening programs.

  4. Intra-patient semi-automated segmentation of the cervix-uterus in CT-images for adaptive radiotherapy of cervical cancer

    Science.gov (United States)

    Luiza Bondar, M.; Hoogeman, Mischa; Schillemans, Wilco; Heijmen, Ben

    2013-08-01

    For online adaptive radiotherapy of cervical cancer, fast and accurate image segmentation is required to facilitate daily treatment adaptation. Our aim was twofold: (1) to test and compare three intra-patient automated segmentation methods for the cervix-uterus structure in CT-images and (2) to improve the segmentation accuracy by including prior knowledge on the daily bladder volume or on the daily coordinates of implanted fiducial markers. The tested methods were: shape deformation (SD) and atlas-based segmentation (ABAS) using two non-rigid registration methods: demons and a hierarchical algorithm. Tests on 102 CT-scans of 13 patients demonstrated that the segmentation accuracy significantly increased by including the bladder volume predicted with a simple 1D model based on a manually defined bladder top. Moreover, manually identified implanted fiducial markers significantly improved the accuracy of the SD method. For patients with large cervix-uterus volume regression, the use of CT-data acquired toward the end of the treatment was required to improve segmentation accuracy. Including prior knowledge, the segmentation results of SD (Dice similarity coefficient 85 ± 6%, error margin 2.2 ± 2.3 mm, average time around 1 min) and of ABAS using hierarchical non-rigid registration (Dice 82 ± 10%, error margin 3.1 ± 2.3 mm, average time around 30 s) support their use for image guided online adaptive radiotherapy of cervical cancer.

  5. Comparative analysis of cisplatin-induced nephrotoxicity in head and neck cancer and carcinoma cervix during concurrent chemoradiotherapy

    Directory of Open Access Journals (Sweden)

    Puneet Kumar Bagri

    2014-01-01

    Full Text Available Background: Cisplatin is widely used as radio sensitizer in head and neck cancer (HNC and carcinoma cervix (CaCx. This study aims to see comparative nephrotoxicity of cisplatin in HNC and in CaCx without obstructive uropathy treated by concurrent chemoradiotherapy (CCRT. Materials and Methods: Fifty patients of HNC and 50 patients of CaCx stage II/III without obstructive uropathy were included in this study. Cisplatin 50 mg intravenous weekly was given before EBRT with adequate hydration and premedication in both groups. Before chemotherapy; blood urea, serum creatinine, and glomerular filtration rate (GFR were measured. GFR was measured using 99m Tc diethylene triamine pentacaetic acid (DTPA renogram study. Results: At the end of 4 th week, blood urea level 41-45 mg% was in 40 and 4% in HNC and CaCx, respectively (P = 0.018. At the end of 3 rd and 4 th week, blood urea level >45 mg% was 10 and 6% in HNC cases, respectively. At the end of 4 th week, serum creatinine level 1.1-1.5 mg% was 50 and 8% in HNC and CaCx, respectively (P = 0.047. Serum creatinine level >1.5 mg% was 6, 8, and 22% in HNC at the end of 2 nd , 3 rd , and 4 th week, respectively. GFR <80 ml/min at the end of 4 th week was 14% in HNC and only 2% in CaCx. GFR <100ml/min was significant at the end of 4 th week (P = 0.04. Univariate analysis showed significant relation between reduced oral fluid intake and reduced GFR (P < 0.001. Conclusion: In HNC, during concurrent chemoradiation, as the 3 rd -4 th week is reached, oral mucosal reactions increase and affect oral intake which further add to the cisplatin-induced nephrotoxicity. In CaCx without obstructive uropathy, renal function impairment is less severe as oral intake of water and liquid is not much impaired.

  6. Histologia e morfometria das glândulas das junções infundíbulo-magno e útero-vagina de codorna doméstica Histology and morphometry of the infundibulum-magnum and uterus-vaginal junctions of spotted quails

    Directory of Open Access Journals (Sweden)

    Carime Moraes

    2009-04-01

    Full Text Available A análise comparativa das glândulas armazenadoras de espermatozóides das junções infundíbulo-magno e útero-vagina do oviduto da codorna Nothura maculosa foi realizada durante a fase reprodutiva. As aves foram eutanasiadas por inalação com éter etílico, após a pesagem. Após a laparotomia e o deslocamento cranial do esterno, foram coletadas amostras de dois centímetros da junção infundíbulo-magno e útero-vagina. Os fragmentos foram fixados em solução de Bouin por 24 horas e, posteriormente, banhos sucessivos de álcool 70% foram aplicados sobre as amostras. Em seguida, as amostras foram desidratadas em uma série de concentração crescente de alcoóis (80, 90, 95 e 3x100%, diafanizadas em xilol e incluídas em parafina. Cortes histológicos de sete micrômetros de espessura foram obtidos e corados pela técnica da Hematoxilina-eosina (HE, que foram analisados e fotomicrografados em um fotomicroscópio Olympus BX-50. Para a análise morfométrica, foram capturadas imagens das glândulas armazenadoras de espermatozóides da junção infundíbulo-magno e útero-vagina mediante o programa computacional "Image Pro Plus 4.1" da Cibernetics do Brasil, tornando-se possível a quantificação das mesmas. A mucosa da junção infundíbulo-magno apresentou pregas estreitas no infundíbulo e largas no magno, enquanto na junção útero-vagina mostrou-se amplamente pregueada na vagina e mais lisa na porção uterina. O número médio de glândulas armazenadoras de espermatozóides na junção infundíbulo-magno foi maior (11,7 glândulas (PThe comparative analysis of sperm-host glands of infundibulum-magnum and uterus-vagina junctions of Nothura maculosa quail oviduct was done during the reproductive phase. The birds were euthanized with ethyl ether after weight data were taken. After abdominal laparotomy and cranial displacement of the breastbone, two centimeters samples of infundibulum-magnum and uterus-vagina junctions were collected

  7. Combinatorial effects of genistein and sex-steroids on the level of cystic fibrosis transmembrane regulator (CFTR), adenylate cyclase (AC) and cAMP in the cervix of ovariectomised rats.

    Science.gov (United States)

    Salleh, Naguib; Ismail, Nurain; Muniandy, Sekaran; Korla, Praveen Kumar; Giribabu, Nelli

    2015-12-01

    The combinatorial effects of genistein and estrogen (E) or estrogen plus progesterone (E+P) on CFTR, AC and cAMP levels in cervix were investigated. Ovariectomised adult female rats received 50 or 100mg/kg/day genistein with E or E followed by E+P [E+(E+P)] for seven consecutive days. Cervixes were harvested and analyzed for CFTR mRNA levels by Real-time PCR. Distribution of AC and CFTR proteins in endocervix were observed by immunohistochemistry. Levels of cAMP were measured by enzyme-immunoassay. Molecular docking predicted interaction between genistein and AC. Our results indicate that levels of CFTR, AC and cAMP in cervix of rats receiving genistein plus E were higher than E-only treatment (pCFTR, AC and cAMP in cervix of E and E+(E+P)-treated rats by genistein could affect the cervical secretory function which could influence the female reproductive processes.

  8. Dissection of the sentry ganglion by laparoscopic boarding in patients with cervix uterine cancer clinical stages IA2 at IIB; Diseccion de ganglio centinela por abordaje laparoscopico en pacientes con cancer cervicouterino etapas clinicas IA2 a IIB

    Energy Technology Data Exchange (ETDEWEB)

    Valdez U, J.J.; Pichardo M, P.A.; Cortes M, G.; Escudero de los Rios, P. [Hospital de Oncologia. Centro Medico Nacional Siglo XXI. IMSS, Mexico D.F. (Mexico)

    2005-07-01

    The obtained results in presently study demonstrate that the feasibility of the detection of the sentry ganglion in cervix uterine cancer using a boarding by laparoscopic via, being necessary the use of twice labelled as much with patent blue and radioisotope (colloid of labelled rhenium with {sup 99m}Tc, total dose of 3 MCi) to achieve the identification of the ganglion. (Author)

  9. Vaginal sponge and spermicides

    Science.gov (United States)

    ... in the vagina, cervix, or uterus Had an abortion, miscarriage, or a baby How well does the ... of Gynecology and Obstetrics at Johns Hopkins University School of Medicine, Baltimore, MD. Review provided by VeriMed ...

  10. Certain Bacteria May Affect Preterm Birth Risk

    Science.gov (United States)

    ... https://medlineplus.gov/news/fullstory_163401.html Certain Bacteria May Affect Preterm Birth Risk Bad 'bugs' tied ... Feb. 3, 2017 (HealthDay News) -- Certain types of bacteria in a pregnant woman's cervix and vagina can ...

  11. Biopsy - polyps

    Science.gov (United States)

    Polyp biopsy ... are treated is the colon. How a polyp biopsy is done depends on the location: Colonoscopy or flexible sigmoidoscopy explores the large bowel Colposcopy-directed biopsy examines the vagina and cervix Esophagogastroduodenoscopy (EGD) or ...

  12. Photodynamic Therapy

    Science.gov (United States)

    ... types of cancer and pre-cancerous conditions, including cancers of the: Skin Cervix Bladder Prostate Bile duct Pancreas Stomach Brain Mouth Larynx (voice box) Vagina Vulva (the outside part of ...

  13. Vestcovaginal fistula after radiotherapy of uterine cervix cancer%宫颈癌放疗后膀胱阴道瘘10例临床分析

    Institute of Scientific and Technical Information of China (English)

    李清秀

    2009-01-01

    Objective To explore the treatment on vesieovaginal fistula (VVF) after radiotherapy of uterine cervix cancer. Methods Ten cases of VVF after radiotherapy of uterine cervix cancer were analyzed retrospectively. All patients were diagnosed by eystoscope and methyhhioninium chloride test. Two eases were diagnosed after curetive radiotherapy and other 8 cases after adjuvant radiotherapy. The VVFs were diagnosed at 3~58 months after radiotherapy. Results The VVFs were repaired at 4~ 16 months after diagnosis. 6 cases were successfully repaired, 3 were given urinary diversion (1 case for tumor locally relapse and 2 cases for severe radioactive damage) and 1 ease failed for tumor relapse and locally infiltrate. Conclusions The repair of VVF after radiotherapy of uterine cervix cancer is difficult. The timing of repair and controlling of tumor are important for the operation outcome. Urinary diversion should be considered in patients with severe radioactive damage or local tumor relapse.%目的 探讨宫颈癌放疗后并发膀胱阴道瘘的处理.方法 回顾性分析1995年1月至2009年1月收治的10例官颈癌放疗后并发膀胱阴道瘘患者的临床资料.尿瘘出现于宫颈癌放疗后3~58个月,2例为根治性放疗出现膀胱阴道瘘,另外8例于术后辅助放疗时出现,均经膀胱镜检查和亚甲蓝试验确诊.于尿瘘后4~16个月行手术修补.结果 6例手术修补成功;2例由于放射性损伤严重而行尿流改道,1例先行直接修补后因肿瘤局部复发再次出现膀胱阴道瘘而行尿流改道术;1例由于肿瘤复发而修补失败.结论 官颈癌放疗后出现的膀胱阴道瘘修补难度大,修补时间及肿瘤控制情况影响修补成功率;部分放射性损伤严重或有局部肿瘤复发的患者可以考虑行尿流改道手术.

  14. Effect of Saffron (Fan Hong Hua) On the Readiness of The Uterine Cervix In Term Pregnancy: A Placebo-Controlled Randomized Trial

    Science.gov (United States)

    Sadi, Roghaieh; Mohammad-Alizadeh-Charandabi, Sakineh; Mirghafourvand, Mojgan; Javadzadeh, Yousef; Ahmadi-Bonabi, Afkham

    2016-01-01

    Background Readiness of the cervix is required for successful induction of labor, and there are a number of ways in which this can be achieved. Objectives The aim of the present study was to assess whether taking saffron orally could have an effect on the cervical readiness (primary outcome) and some delivery and neonatal outcomes (secondary outcomes) in women in the stage of term pregnancy. Patients and Methods In this double-blind trial, 50 women with a gestational age of 39 to 41 weeks, no indication of cesarean section, a Bishop’s score of less than 4, who had plan to have vaginal delivery at Shohada hospital of Bonab, Iran were randomized into two groups receiving three 250mg saffron or placebo pills in 24 hours. The readiness of the cervix was assessed using the Bishop’s score. The Mann-Whitney U test was used to compare the scores between the groups. Results There were no significant differences between the groups in terms of the Bishop’s score at the baseline (P = 0.792) and 10-12 hours after starting the intervention (P = 0.159). The Bishop’s score was significantly higher in the saffron group 20 - 24 hours after the intervention was started (P = 0.029) and just after onset of active uterine contractions (P = 0.003). In the saffron group, there was no cesarean section and 1 meconium staining of the fetus, but 3 and 4, respectively, in the placebo group. There was no statistically significant difference between the groups in terms of the timing of the onset of spontaneous active uterine contractions, the duration of the first and second stages of labor, the need for delivery augmentation, and the first and fifth minutes of neonatal Apgar (P > 0.05). No adverse event was reported in any of the groups. Conclusions Saffron can increase the readiness of the cervix in term pregnancies. However, the study limitations do not allow for any definite conclusions for its use in clinical practice, and more research is needed to assess its effect on delivery and

  15. Body mass index versus bladder and rectal doses using 2D planning for patients with carcinoma of the cervix undergoing HDR brachytherapy

    Directory of Open Access Journals (Sweden)

    Anil Kumar Talluri

    2016-06-01

    Full Text Available Purpose: To assess bladder and rectum doses in relation to body mass index of patients undergoing high dose rate brachytherapy for the treatment of carcinoma of the cervix.Methods: The cohort consists of fifty subjects with carcinoma of the uterine cervix presented with grade II and III. Patient’s height and weight was measured before the insertion of applicator in situ. Body mass index (BMI of the patient was calculated in accordance to World Health Organization definition (weight in Kg/ height in m2. Adequacy of position and orientation of the applicator was confirmed with the help of orthogonal X-ray images and the same were transferred to the treatment planning system (TPS to generate treatment plan. Prescription doses were optimized to Point A and to reference lines placed at 0.5 cm apart from the surface of ovoids. The following dose reference points were identified on orthogonal x-ray images for analysis using the rectal marker and Foleys bulb inflated with radio opaque dye Rectal points at the level of femoral heads (RL and pubis symphysis (RLP, Anorectum Junction (AR Jn point and Rectosigmoid (RS point and Bladder point (BL. Pearson regression analysis was used to analyze data from TPS.Results: The mean BMI was 22.7 kg/m2 and average age was 49.9 years. Analysis showed that RL point dose and BMI were inversely correlated with a coefficient -0.45 (p = 0.001. The trend continued along the rectal tube in cranio-caudal direction, as RLP and AR Jn points showed inversion co-efficiency with increase in BMI,-0.48 (p < 0.01 and -0.51 (p < 0.01 respectively. Bladder point showed weak positive correlation to BMI, 0.12 (p = 0.38.Conclusion: Significant rectal dose reduction is observed with increase in BMI. Bladder dose did not show statistically significant correlation with BMI. Based on the findings, BMI constitutes a confounding factor in the treatment of carcinoma of cervix.

  16. Influence of different prostaglandin applications on cervical rheology.

    Science.gov (United States)

    Spätling, L; Neuman, M R; Huch, R; Huch, A

    1985-10-01

    The softening effect of prostaglandin (PG) on cervical tissue prior to elective pregnancy termination is quantified by a new technique for the measurement of the elastance and relaxation of the cervix. The method is based on the pressure-volume relation of a compliant balloon placed in the cervical canal. These properties have been measured before and after different applications of prostaglandins in 58 patients electively terminating pregnancy. Application techniques used included high pressure jet application of PGE2 into the tissue of the portio uteri and the internal cervical os (120 micrograms), PGE2 and PGF2 alpha in Tylose gel (100 micrograms/0.5 ml); PGE2 as an intracervical tablet (150 micrograms) and PGE2 oral tablets placed into the posterior fornix of the vagina. Significant changes in cervical elastance were seen with the intracervically applied PGE2 in Tylose gel and the vaginally applied PGE2 tablets. The intracervically applied PGE2 gel also gave significant changes in cervical relaxation. No side effects other than mild cramping (2 patients) were seen with any of the applications in this study.

  17. Relationship of microecological environmental changes of vagina and two kinds of vaginitis%女性阴道微生态变化与两种阴道炎的关系

    Institute of Scientific and Technical Information of China (English)

    杨岚; 邵兰芬; 陈道桢

    2011-01-01

    Objective To investigate the relationship of microecological environment of vagina and vulvovaginal candidiasis (VVC) and trichomonas vaginitis (TV) of women. Methods The examinations of hydrogen peroxide, sialidase and leukocyte esterase in vaginal secretions were performed with routine smear and BV triple test in 2380 cases with WC (group WC), 1399 cases with TV( group TV) and 648 healthy controls (group C). Results The positive rates of leukocyte esterase in groups of WC and TV were 34. 7% and 61. 5% .respectively,which were both higer than 6. 6% in group C(P<0. 05). The positive rate was higher in group TV than that in group VVC (P<0. 05). Conclusion The occurance of WC and TV is closely related to the unbanlance of vaginal microecological environment Examining vaginal secretions with routine smear and Bvset, especially with leukocyte esterase test,should be taken for guiding the microecological evaluation and controlling the recurrence of vaginitis.%目的 了解外阴阴道假丝酵母菌病(VVC)及滴虫性阴道炎(Tv)与阴道微生态情况的关系.方法 用常规检测和细菌性阴道病(BV)三联法(BVset)测定2380例WC(WC组)、1399例TV患者(TV组)和648例正常体检者(对照组)阴道分泌物中过氧化氢(H2O2)浓度、唾液酸苷酶和白细胞酯酶活性.结果 VVC和TV患者三联法检测白细胞酯酶阳性率分别为34.7%和61.5%,明显高于对照组的6.6%(P<0.05);且TV组的白细胞酯酶阳性率显著高于WC组(P<0.05).结论 VVC和TV的发生与阴道微生态环境的失衡密切相关;对患者进行白带常规检测的同时进行BVset尤其是白细胞酯酶活性测定,能更好地反映出患者阴道微生态状况,对控制阴道炎的复发有一定的指导意义.

  18. Chemopreventive effects of Cuminum cyminum in chemically induced forestomach and uterine cervix tumors in murine model systems.

    Science.gov (United States)

    Gagandeep; Dhanalakshmi, Sivanandhan; Méndiz, Ester; Rao, Agra Ramesha; Kale, Raosaheb Kathalupant

    2003-01-01

    Lately, a strong correlation has been established between diet and cancer. For ages, cumin has been a part of the diet. It is a popular spice regularly used as a flavoring agent in a number of ethnic cousins. In the present study, cancer chemopreventive potentials of different doses of a cumin seed-mixed diet were evaluated against benzo(a)pyrene [B(a)P]-induced forestomach tumorigenesis and 3-methylcholanthrene (MCA)-induced uterine cervix tumorigenesis. Results showed a significant inhibition of stomach tumor burden (tumors per mouse) by cumin. Tumor burden was 7.33 +/- 2.10 in the B(a)P-treated control group, whereas it reduced to 3.10 +/- 0.57 (P < 0.001) by a 2.5% dose and 3.11 +/- 0.60 (P <0.001) by a 5% dose of cumin seeds. Cervical carcinoma incidence, compared with the MCA-treated control group (66.67%), reduced to 27.27% (P < 0.05) by a diet of 5% cumin seeds and to 12.50% (P < 0.05) by a diet of 7.5% cumin seeds. The effect of 2.5 and 5% cumin seed-mixed diets was also examined on carcinogen/xenobiotic metabolizing phase I and phase II enzymes, antioxidant enzymes, glutathione content, lactate dehydrogenase (LDH), and lipid peroxidation in the liver of Swiss albino mice. Levels of cytochrome P-450 (cyt P-450) and cytochrome b5 (cyt b(5)) were significantly augmented (P < 0.05) by the 2.5% dose of cumin seed diet. The levels of cyt P-450 reductase and cyt b(5) reductase were increased (significance level being from P < 0.05 to P < 0.01) by both doses of cumin. Among the phase II enzymes, glutathione S-transferase specific activity increased (P < 0.005) by the 5% dose, whereas that of DT-diaphorase increased significantly (P < 0.05) by both doses used (2.5 and 5%). In the antioxidant system, significant elevation of the specific activities of superoxide dismutase (P < 0.01) and catalase (P < 0.05) was observed with the 5% dose of cumin. The activities of glutathione peroxidase and glutathione reductase remained unaltered by both doses of cumin. The level

  19. Implementation of a High-Dose-Rate Brachytherapy Program for Carcinoma of the Cervix in Senegal: A Pragmatic Model for the Developing World

    Energy Technology Data Exchange (ETDEWEB)

    Einck, John P., E-mail: jeinck@ucsd.edu [Department of Radiation Medicine and Applied Sciences, University of California San Diego, San Diego, California (United States); Hudson, Alana [Department of Oncology, Tom Baker Cancer Centre, University of Calgary, Calgary, Alberta (Canada); Shulman, Adam C. [Overlook Medical Center, Summit, New Jersey (United States); Yashar, Catheryn M. [Department of Radiation Medicine and Applied Sciences, University of California San Diego, San Diego, California (United States); Dieng, Mamadou M.; Diagne, Magatte; Gueye, Latifatou; Gningue, Fama; Gaye, Pape M. [Départemént de Radiothérapie, Institut Joliot-Curie, Hôpital Aristide Le Dantec, Dakar (Senegal); Fisher, Brandon J. [GammaWest Cancer Services, Salt Lake City, Utah (United States); Mundt, Arno J. [Department of Radiation Medicine and Applied Sciences, University of California San Diego, San Diego, California (United States); Brown, Derek W. [Department of Oncology, Tom Baker Cancer Centre, University of Calgary, Calgary, Alberta (Canada)

    2014-07-01

    West Africa has one of the highest incidence rates of carcinoma of the cervix in the world. The vast majority of women do not have access to screening or disease treatment, leading to presentation at advanced stages and to high mortality rates. Compounding this problem is the lack of radiation treatment facilities in Senegal and many other parts of the African continent. Senegal, a country of 13 million people, had a single {sup 60}Co teletherapy unit before our involvement and no brachytherapy capabilities. Radiating Hope, a nonprofit organization whose mission is to provide radiation therapy equipment to countries in the developing world, provided a high-dose-rate afterloading unit to the cancer center for curative cervical cancer treatment. Here we describe the implementation of high-dose-rate brachytherapy in Senegal requiring a nonstandard fractionation schedule and a novel treatment planning approach as a possible blueprint to providing this technology to other developing countries.

  20. Evaluation of socio-demographic factors for non-compliance to treatment in locally advanced cases of cancer cervix in a rural medical college hospital in India

    Directory of Open Access Journals (Sweden)

    Samrat Dutta

    2013-01-01

    Full Text Available Introduction: Carcinoma cervix is a leading cause of cancer in India. However, majority of the patients face a problem of not being able to complete the treatment. Aim: This study was an attempt to find out the important causes of this non-compliance to treatment in a rural Medical College Hospital where majority of the cancer cases are of cervical cancer. Results: Out of 144 patients studied over 2 years 88 cases could not complete the treatment. The study revealed that due old age 58.33% cases were defaulters, having many children at home meant a burden to 76.92% cases and 63.89% cases had a problem of not been able to travel a far distance of more than 100 km from home to hospital for treatment. Conclusion: These were the important factors of non-compliance and suggested more important than the issues of literacy and poor socio-economic status.

  1. Implementation of a high-dose-rate brachytherapy program for carcinoma of the cervix in Senegal: a pragmatic model for the developing world.

    Science.gov (United States)

    Einck, John P; Hudson, Alana; Shulman, Adam C; Yashar, Catheryn M; Dieng, Mamadou M; Diagne, Magatte; Gueye, Latifatou; Gningue, Fama; Gaye, Pape M; Fisher, Brandon J; Mundt, Arno J; Brown, Derek W

    2014-07-01

    West Africa has one of the highest incidence rates of carcinoma of the cervix in the world. The vast majority of women do not have access to screening or disease treatment, leading to presentation at advanced stages and to high mortality rates. Compounding this problem is the lack of radiation treatment facilities in Senegal and many other parts of the African continent. Senegal, a country of 13 million people, had a single (60)Co teletherapy unit before our involvement and no brachytherapy capabilities. Radiating Hope, a nonprofit organization whose mission is to provide radiation therapy equipment to countries in the developing world, provided a high-dose-rate afterloading unit to the cancer center for curative cervical cancer treatment. Here we describe the implementation of high-dose-rate brachytherapy in Senegal requiring a nonstandard fractionation schedule and a novel treatment planning approach as a possible blueprint to providing this technology to other developing countries.

  2. Inappropriate cervical injection of radiotracer for sentinel node mapping in a uterine cervix cancer patient: importance of lymphoscintigraphy and blue dye injection

    Directory of Open Access Journals (Sweden)

    Sima Kadkhodayan

    2014-10-01

    Full Text Available Herein, we report a case of sentinel lymph node mapping in a uterine cervix cancer patient, referring to the nuclear medicine department of our institute. Lymphoscintigraphy images showed inappropriate intra‐cervical injection of radiotracer. Blue dye technique was applied for sentinel lymph node mapping, using intra‐cervical injection of methylene blue. Two blue/cold sentinel lymph nodes, with no pathological involvement, were intra‐operatively identified, and the patient was spared pelvic lymph node dissection. The present case underscores the importance of lymphoscintigraphy imaging in sentinel lymph node mapping and demonstrates the added value of blue dye injection in selected patients. It is suggested that preoperative lymphoscintigraphy imaging be considered as an integral part of sentinel lymph node mapping in surgical oncology. Detailed results of lymphoscintigraphy images should be provided for surgeons prior to surgery, and in case the sentinel lymph nodes are not visualized, use of blue dye for sentinel node mapping should be encouraged.

  3. Effectiveness of extra-ovular injection of prostaglandin E2 in tylose gel to ripen the cervix prior to elective induction of labor at term.

    Science.gov (United States)

    Thiery, M; Defoort, P; Benijts, G; Van Eyck, J; Hennay, T; Van Kets, H; Martens, C

    1977-08-01

    Ripening of the unfavorable cervix (Bishop score less than or equal to 4) was obtained in 92 clinically normal gravidae at term (68 nulliparae and 24 parous women), not in labor and with intact membranes, by injecting one or two doses (250 to 500 mug each) of prostaglandin (PG)E2 suspended in a viscous gel (5% Tylose) into the extra-ovular space. On average 7 to 8 hours after the injection the mean increase of the cervical score was 3.7 and 4.1 in the nulliparous and parous women, respectively. Complications associated with placement of the catheter were few. The method is simple, well tolerated and no untoward maternal or perinatal effects could be directly attributed to it. However, suitable criteria for predicting both the effect of the procedure and the optimal PG dose to be administered are still needed.

  4. Do sensory calcitonin gene-related peptide nerve fibres in the rat pelvic plexus supply autonomic neurons projecting to the uterus and cervix?

    Science.gov (United States)

    Houdeau, E; Barranger, E; Rossano, B

    2002-10-25

    Sensory nerve fibres containing calcitonin gene-related peptide (CGRP) innervate neurons of the paracervical ganglion (PCG) in the female rat pelvic plexus. We have combined retrograde tracing with immunocytochemistry to investigate whether CGRP-immunoreactive (-IR) fibres supply neurons targeting the genital tract. Of the total neurons projecting to either the uterine horns or the cervix, 38 and 41% received CGRP-IR innervation, respectively. All these neurons displayed choline acetyltransferase-IR, thus are cholinergic. They were found throughout the PCG and other pelvic plexus ganglia, namely accessory ganglia (AG) and hypogastric plexus (HP). Pelvic nerve section showed that afferent fibres in these nerves provided most of the CGRP-IR fibres supplying uterine- or cervical-related neurons in the PCG/AG, none in HP. It is suggested that such sensory-motor network may provide a local pathway for reflex control of genital tract activity, acting through cholinergic nerve projections.

  5. The major predisposing factors to human papilloma virus (HPV infection among patients with cancer of the cervix and their knowledge of the disease

    Directory of Open Access Journals (Sweden)

    Lynn Lipke

    1997-02-01

    Full Text Available Objective: The purpose of this study was to identify the main predisposing factors to human papilloma virus (HPV infection among patients with cancer of the cervix as well as establish their knowledge and awareness of the disease, so as to design educational programmes to minimise the future occurrence of the disease.

    Opsomming
    Doel: Die doel van hierdie studie was om die vernaamste predisposionele faktore tot Menslike Papilloma Virus (HPV infeksie te identifiseer by pasiente met kanker van die serviks, insluitende hulle kennis en bewustheid van die siekte, ten einde opvoedkundige programme tot stand te bring en te ontwerp wat die hoe voorkoms van die siekte kan minimiseer. *Please note: This is a reduced version of the abstract. Please refer to PDF for full text.

  6. Comparison of Southern blot analysis with isotopic and nonisotopic in situ hybridization for the detection of human papillomavirus sequences in invasive carcinoma of the uterine cervix.

    Science.gov (United States)

    D'Amato, L; Pilotti, S; Rotola, A; Di Luca, D; Cassai, E; Rilke, F

    1992-03-01

    To compare the efficiency of hybridization methods for the detection of HPV genome, 22 cases of invasive squamous cell carcinoma of the uterine cervix were analyzed by Southern blot analysis and in situ hybridization carried out with 35S- and biotin-labeled probes. These cases contained from less than one to as many as 50 copies per cell of HPV 16 and 18 types. To increase the sensitivity of biotinylated probes, a silver enhancement procedure of the peroxidase reaction product was applied. Results showed that in situ hybridization performed with isotopic probes is as sensitive as Southern blot analysis and is more sensitive than that performed with biotin-labeled probe. However, the application of the silver enhancement procedure increases the percentage of HPV-positive cases from 27 to 50%.

  7. 心理干预对先天性无阴道患者术后婚姻和生活质量的影响%The influence on the quality of marital and life in the congenital absence of vagina patients with operation by psychological intervention

    Institute of Scientific and Technical Information of China (English)

    张晓玲; 朱清仙

    2008-01-01

    目的 探讨心理干预对先天性无阴道患者术后婚姻和生活质量的影响.方法 将先天性无阴道患者按自愿原则分为干预组18例和非干预组11例,对干预组的患者进行术前的认知行为治疗,用Olson婚姻质量调查问卷及诺丁汉健康量表对两组手术前后生活质量以及术后婚姻质量进行评估.结果:干预组术后婚姻和生活质量评分均显著优于非干预组(P<0.05或P<0.01).结论 手术前心理干预能有效提高先天性无阴道患者术后婚姻和生活质量.%Objective To explore the influence on the quality of marital and life of the congenital absence of vagina patients with operation by psychological intervention.Methods The congenital absente of vagina patients were divided into psycholOgical intervened group(n=18,all cases were treated with the cognitive and behaviour therapy)and without intervened group(n=11).Before and after treatment the scores of the nottingham health poofity(NHP)were used in the two groups.After treatment the Olson's enrich questionary were tested also.Results The scores of NHP and Olson of psychological intervened group were significantly superior to those without intervened group(P<0.05 or P<0 01).Conclusion The cogntive and behaviour theraopy before operation is good for the quality of marital and life in congenital absence of vagina patients.

  8. Radical radiotherapy treatment (EBRT + HDR-ICRT of carcinoma of the uterine cervix: Outcome in patients treated at a rural center in India

    Directory of Open Access Journals (Sweden)

    Jain Vandana

    2007-01-01

    Full Text Available Aim: To report the outcome of carcinoma of the uterine cervix patients treated radically by external beam radiotherapy (EBRT and high-dose-rate (HDR intracavitary radiotherapy (ICRT. Materials and Methods: Between January 1997 to December 2001, a total of 550 newly diagnosed cases of carcinoma of the uterine cervix were reported in the department. All cases were staged according to the International Federation of Gynecologists and Oncologists (FIGO staging system, but for analytical convenience, the staging was limited to stages I, II, III, and IV. Out of the 550 cases, 214 completed radical radiotherapy (EBRT + HDR-ICRT and were retrospectively analyzed for presence of local residual disease, local recurrence, distant metastases, radiation reactions, and disease-free survival. Results: There were 7 (3.27%, 88 (41.1%, 101 (47.1%, and 18 (8.4% patients in stage I, II, III, and IV, respectively. The median follow-up time for all patients was 43 months (range: 3-93 months and for patients who were disease free till the last follow-up it was 59 months (range: 24-93 months. The overall treatment time (OTT ranged from 52 to 73 days (median 61 days. The 5-year disease-free mean survival rate was 58%, 44%, 33%, and 15%, with 95% confidence interval of 48 to 68, 37 to 51, 24 to 35, and 6 to 24 for stages I, II, III, and IV, respectively. There were 62 (28.97% cases with local residual disease, 35 (16.3% developed local recurrence/distant metastases, 17 (7.9% developed distant metastases, and 9 (4.2% had local recurrence as well. Discussion and Conclusion: The overall outcome was poor in advanced stage disease, but might be improved by increasing the total dose, decreasing overall duration of treatment, and by adding chemotherapy in patients with disease limited to the pelvis.

  9. A PROSPECTIVE STUDY FOR EVALUATING VISUAL INSPECTIO N AFTER ACETIC ACID (VIA AND LUGOLS IODINE (VILI APPLICAT ION IN SCREENING OF PREMALIGNANT LESIONS OF CERVIX

    Directory of Open Access Journals (Sweden)

    Kalpana

    2013-01-01

    Full Text Available ABSTRACT: AIMS AND OBJECTIVES: To evaluate the potential of VIA & VILI in the scre ening of preinvasive and invasive lesions in unhealthy cervix and comparing them with colposcopy guided biopsy thereby determining its usefulness in screening. Also to evaluate the correlation of demographic data like age, socioeconomic status, education, residential area, parity, age at marriage, use of various contraceptive methods in pre malignant lesions of cervix. METHODS STUDY was conducted on 200 symptomatic and asymptomatic w omen with abnormal cervices who attended outdoor department of SSMC associated h ospital SGMH. All cases were subjected to Pap smear, VIA, VILI and colposcopy, women with prem alignant lesion were subjected to directed biopsy from suspicious areas and sent for h istopathological examination (HPE. Results of Pap smear, VIA, VILI and colposcopy are compared to HPE report. OBSERVATIONS: On cytology 27.5% of cases had LSIL & HSIL. All were po sitive by VIA & VILI, of which 80% had dysplasia on biopsy. On colposcopy, incidence of CIN wa s 38.5%. all were positive on VIA & VILI, out of which, 76.6% were proved dysplastic on colposco py guided biopsy. VIA when compared with HPR had sensitivity & specificity of 80% & 67% respectively, while that of VILI was found to be 80% & 87% respectively. CONCLUSION: VIA & VILI are simple, inexpensive, low technology test. Both when combined has high sensit ivity as well as specificity. This can be practiced by clinicians and paramedics on wide scale. Another advantage is immediate availability of results, so that treatment can be s tarted during same visit.

  10. Cervical occlusion in women with cervical insufficiency: protocol for a randomised, controlled trial with cerclage, with and without cervical occlusion

    DEFF Research Database (Denmark)

    Secher, Niels Jørgen; MaCormack, CD; Weber, Tom;

    2007-01-01

    , without the membranes being exposed to the vagina. 6. Tertiary cerclage: Short cervix, membranes exposed to the vagina. Observational study: Eligible women who refuse to be randomised will participate in an observational study. 7. Repeat/requested cervical occlusion. METHODS: The women will be randomised...

  11. Prevalência de cândida na flora vaginal de mulheres atendidas num serviço de planejamento familiar Prevalence of candida in the vagina of women attended at a family planning service

    Directory of Open Access Journals (Sweden)

    Antônio Aleixo Neto

    1999-01-01

    Full Text Available RESUMO Objetivo: avaliar a prevalência de Candida sp. e a distribuição de suas espécies na flora vaginal de mulheres numa clínica de planejamento familiar. Método: estudo transversal no qual se avaliaram prospectivamente 72 mulheres não-grávidas, com ou sem queixas específicas, sendo coletadas amostras de secreção vaginal para cultura de leveduras, efetuada a medição do pH vaginal e anotados dados de achados do exame ginecológico. Resultados: leveduras pertencentes ao gênero Candida foram encontradas em 18 casos (25%. A C. albicans foi a espécie mais prevalente (77,8% e conseqüentemente 22,2% foram não-albicans. Entre as não-albicans a espécie mais prevalente foi a C. glabrata (16,7% seguida pela C. parapsilosis (5,6%. Prurido e ardor foram os únicos sintomas relacionados significativamente com a presença de cândida. Foi observado que a C. glabrata, ao contrário da C. albicans, não causa corrimento clinicamente verificável ao exame ginecológico. Não foram verificadas associações de alguns fatores predisponentes (idade, escolaridade e uso de contraceptivos com a presença ou não de cândida. Conclusões: nossos resultados sugerem: a uma alta prevalência de Candida sp. entre as mulheres (25%; b que as espécies não-albicans desempenham um papel importante no meio vaginal; c que prurido e ardor são os sintomas mais comuns na presença de cândida e d que a C. glabrata não costuma causar corrimento vaginal ao exame ginecológico. Finalmente, é importante observar que nossos resultados são consistentes com o que a literatura internacional tem mostrado nos últimos anos.SUMMARY Purpose: to estimate the prevalence of Candida sp. and the distribution of its species in the vagina of women attended at a family planning Service. Methods: a cross-sectional study evaluating prospectively 72 nonpregnant women, with or without specific complaints. Samples were checked for the presence of yeast and vaginal pH. Data obtained

  12. Molecular ecological structure of fungal flora in the vaginas of patients with diabetes mellitus%糖尿病患者阴道真菌菌群结构与生态分布研究

    Institute of Scientific and Technical Information of China (English)

    郑能能; 吕雯; 周航; 冯国芳; 吕卫国

    2013-01-01

    Objective To characterize the ecological structure of vaginal fungal flora in patients with diabetes mellitus (DM). Methods 18S rDNA sequencing technique was employed for the construction of 18S rDNA clone libraries and phylogenetic tree of vaginal fungal flora in patients with DM and healthy women. Diversity and structural characteristics of fungal flora in each group was then analyzed. Results 3 fungal taxa with 14 genera including 21 phylotypes of fungal flora were found in vaginal specimens. The operational tax-onomic unit (OTUs) which belonged to healthy women (group H), HbA1c 7.5% patients (group DMc) was 7, 9, 13 and 16 respectively. Candida spp. was the most frequently detected clones in each group. An increased proportion of Candida spp. accompanied with decreased proportions of Saccharomyces cerevisi-ae and uncultured fungi was observed in patients with DM. C. albicans, C. glabrata and C. krusei were identified as the predominant species amongst Candida spp. in each group. Conclusion The data revealed a varied ecological structure of vaginal fungal flora in patients with DM and indicated that the degree of disturbed fungal flora in the vaginas of patients with DM might be correlated with the status of glycaemic control.%目的 探讨糖尿病(DM)患者阴道真菌菌群结构和生态分布特点.方法 采用真菌18S rDNA克隆文库法,对不同血糖控制情况下DM患者的阴道真菌18S rDNA序列进行克隆和测序,构建阴道真菌18S rDNA克隆文库,建立系统发育树,获取DM患者阴道真菌菌群的结构和组成特征.结果 DM患者和健康妇女阴道标本中存在3个真菌类群14个属共20个种系型的真菌菌群.健康妇女(H组)、HbA1c<6.5%患者(DMa组)、HbA1c6.5%~7.5%患者(DMb组)和HbA1c>7.5%患者(DMc组)的阴道真菌菌落分别存在7、9、13和16个OTUs.念珠菌属为各组研究对象阴道真菌菌群的优势菌属,其阳性克隆子在H组、DMa组、DMb组和DMc组的比例呈上升

  13. On the Palm in the Vagina Midwifery Clinical Care Application%浅谈助产掌在阴道助产临床护理中的应用

    Institute of Scientific and Technical Information of China (English)

    李安源

    2014-01-01

    目的:为了进一步提高我院妇产科的整体医疗水平,保障产妇和胎儿的生命安全,本文就助产掌在阴道助产临床护理中的应用进行了的简单的研究和探讨。方法将2011年11月至2012年11月期间在我院接受阴道助产掌分娩(观察组)的60例患者的临床资料和同期在我院接受出口产钳助产分娩(对照组)的60例患者的临床资料进行回顾性的分析和对比。结果观察组患者产后12h和24h的出血量明显低于对照组,两组之间的差异显著(P<0.05)。观察组产妇术后感染的发生率也明显低于对照组,两组之间的差异显著(P<0.05)。结论助产掌在阴道助产临床护理过程中的临床价值十分显著,有效的提高了手术的成功概率,降低了手术的风险性,保障了产妇和新生儿的生命安全,值得推广。%Objective To further improve the overall hospital maternity medical standards to protect maternal and fetal safety of this paper palm midwifery midwifery clinical care in the vaginal application of a simple study and discussion. Methods From November 2011 to November 2012 in our hospital during vaginal delivery charge of delivery(study group), 60 patients with clinical data and export the same period in our hospital forceps delivery(control group)60 patients clinical data were retrospectively analyzed and compared. Results The patients 12 h and 24 h post-partum bleeding was significantly lower than the control group, a significant difference between the groups(P<0.05). Also, the observation group maternal incidence of postoperative infection was significantly lower than the control group, a significant difference between the groups(P<0.05). Conclusion Palm midwifery midwifery clinical care in the vagina during the clinical value is very significant, effectively raising the probability of success of surgery, reducing the risk of surgery to protect the lives of mothers and newborns, worthy

  14. 宫颈癌术后盆腔容积调强弧形治疗与固定野调强放疗计划的剂量学研究%Dosimetric study of volumetric intensity-modulated arc therapy and fixed field intensity-modulated radiotherapy for cervix cancer

    Institute of Scientific and Technical Information of China (English)

    杨波; 庞廷田; 孙显松; 胡克; 邱杰; 张福泉

    2012-01-01

    Objective To compare the dosimetry characteristics of volumetric intensity-modulated arc therapy (VIMAT) and fixed field intensity-modulated radiation therapy (FF-IMRT) for cervix cancer.Methods CT images of 13 patients with cervix uteri cancer were transferred into Eclipse planning system.FF-IMRT and VIMAT plans were optimized on an Eclipse treatment planning system using beam data generated for Varian trilogy linear accelerator.Planning target volume (PTV) and organs at risk were evaluated with dose-volume histogram.To appraise the difference between the techniques,the paired t-test was applied.Results Compared with the FF-IMRT plans,PTV95% coverage of VIMAT plan group increased (t =9.84,P =0.000),PTV110% became lower (t =-3.72,P =0.003),Dmax decreased (t =-3.51,P=0.005),and CI became worser (t=5.39,P=0.000).PTV105%,Dmean,and HI had no difference (t =-0.02,-0.60,1.13,P =0.842,0.560,0.283).V30 of the bladder was reduced by about 10% (t =-4.99,P =0.000),and Dmean and Dmax were 1.4 Gy and 1.5 Gy lower respectively (t =-3.65,-18.03,P =0.004,0.000) ;V40 of the rectum was reduced by about 10% (t =-2.99,P =0.012),and Dmean and Dmax were reduced by 0.6 Gy,0.8 Gy respectively (t =-2.98,-4.05,P =0.013,0.002) ;V30,V40 and V50 of the small intestine were reduced by 16%,10% and 11% (t =-10.85,-4.74,-8.66,P =0.000,0.001,0.000),and Dmax was reduced by 0.8 Gy (t =-9.45,P =0.000) ; V30,V40 and V50 of the bone marrow were reduced by 26%,19% and 16% (t =-22.10,-10.19,-4.04,P =0.000,0.000,0.002),and Dmean reduced by 1.9 Gy (t =-16.21,P =0.000) ; D5 of the left and right femoral heads were reduced by 1.6 Gy and 2.7 Gy (t =-2.89,-6.22,P =0.015,0.000).Dmax of the caudate equine was reduced by 1.5 Gy (t=-4.80,P=0.001).V20,V30,V40 and V50 of the body were reduced by 18%,18%,4% and3%(t=-7.52,-11.75,-6.26,-6.94,P=0.000,0.000,0.000,0.000).Dmean and Dmax of the body decreased by 1.0 Gy and 0.4 Gy (t=-3.72,-3.51,P=0.000,0.005).Average machine unit (MU) decreased

  15. Effects of bovine colostrum on the structure of uteri and the expression of estrogen receptor in rat's adult female offsprings%牛初乳对子代雌鼠子宫结构及雌激素受体表达的影响

    Institute of Scientific and Technical Information of China (English)

    徐丽; 张兰威; 张玉梅; 刘钊燕; 吕艳丽

    2013-01-01

    Objective To study the effects of gestational and lactational exposure to bovine colostrum on the structure of uterus and the expression of estrogen receptor (ERα, ERβ) of the female offspring of Spraque-Dawley rats. Methods SD rats were randomly divided into bovine colostrum and control group and fed before mating and during gestation and lactation with bovine colostrum diet and normal diet. Female offsprings were sacrificed after maturity and uteri and ovaries . were removed for weighing. The hormone levels in serum were determined and the uteri were used for pathological examination. Expressions of estrogen receptor α ( ERα) and estrogen β ( ERβ) in uterus were examined using immunohistochemical method. Results There was no significant difference in the female offsprings' body weight and ratio of uterus and ovary weight to body weight between bovine colostrum diet and control diet (P > 0. 05). Bovine colostrum diet significantly increased the level of prolactin (PRL) in serum (P 0. 05 ) . Conclusion The gestational and lactational exposure to bovine colostrum did not cause significant influence on the development of uterus and expressions of ERa and ERβ.%目的 研究妊娠期和哺乳期通过SD母鼠暴露于牛初乳及断乳后继续食用牛初乳对成年子代雌鼠子宫发育及雌激素受体(ERα和ERβ3)表达的影响.方法 将母鼠随机分成2组,牛初乳组和空白对照组.牛初乳组母鼠在交配前期、妊娠期和哺乳期食用含有牛初乳的饲料,仔鼠3周断乳后食用对应于母鼠的饲料至成年.空白组母鼠和仔鼠食用普通饲料.仔鼠成年后处死,取其血液,剥离子宫和卵巢称重,检测血清激素,子宫病理学检查;利用免疫组化法检测子鼠子宫内雌激素受体(ERα和ERβ)的表达.结果 牛初乳组子代雌鼠体重、卵巢和子宫脏体比与空白组相比差异无统计学意义(P>0.05),血清中催乳素(PRL)含量显著高于对照

  16. Associations of Filaggrin Gene Loss-of-Function Variants and Human Papillomavirus-Related Cancer and Pre-Cancer in Danish Adults

    DEFF Research Database (Denmark)

    Skaaby, Tea; Husemoen, Lise Lotte N; Jørgensen, Torben;

    2014-01-01

    to human papillomavirus (HPV) infection and thus a higher risk of HPV-related cancer and pre-cancer. We investigated the association of the FLG genotype with incidence of HPV-related cancer of cervix, vagina, vulva, penis, anus and head and neck, and pre-cancer of the cervix. METHODS: We included 13...

  17. Genital warts

    Science.gov (United States)

    ... warts URL of this page: //medlineplus.gov/ency/article/000886.htm Genital warts To use the sharing ... They may be found on the penis , vulva , urethra, vagina , cervix , and around ... in the cervix , or to cervical cancer . These are called high-risk types of HPV. ...

  18. Analysis of cervix and shoulder pain after anterior cervical operation%颈前路术后颈肩痛分析

    Institute of Scientific and Technical Information of China (English)

    杜文君; 孙娟; 田融; 夏英鹏; 田成瑞; 江毅

    2001-01-01

    目的:观察了颈前路植骨融合术后颈肩痛患者的临床表现,对其原因进行了分析。方法:对28例因颈间盘[摘要]目的:观察了颈前路植骨融合术后颈肩痛患者的临床表现,对其原因进行了分析。方法:对28例因颈间盘突出症及颈椎病患者行“颈前路间盘和/或椎体次全切除取髂骨植骨融合术"后颈肩痛患者临床表现进行了分析,并行X-ray、CT及MRI等影像学检查,对其进行总结。结果:本组病例经证实有植骨块移位、植骨块假关节形成、伴发肩周炎及单纯颈托外固定过紧所致。骨块移位及假关节形成者二次手术复位或切除假关节,追加植骨,钢板内固定,肩周炎及卡压所致者对症治疗,效果均十分满意。结论:颈前路间盘和/或椎体次全切除取髂骨植骨融合术时应注意骨块大小、外固定松紧程度及合并症的早期诊断,为预防颈前路术后颈肩痛的关键。%Objective:The clinic of cervix and shoulder pain after anterior cervical operation has been observed, whose causes have been analysised. Methods: 28 patients with cervix and shoulder pain were analyzed who had been operated on anterior operation of cervical intervertibral disc resection and/ or vertebral body subtotal ectomy and implantation with iliac bone and analyzed their x-ray,CT,and MRI .Results:These patients' pain was caused by implanted bone shift, pseudarthrosis formation, supervention of scapulohumeral periarthritis and tenseness of extra-plastic-collar .The implanted bone shift and pseudarthrosis were operated again with reposition and excision, and implanted more bone, interior fixation with steel-board, expectant treatment with scapulthumeral periarthritis and tenseness of extra-plastic-collar were all satisfied .Conclusion: The anterior operation of cervical intervertibral disc resection and/or vertebral body subtotal ectomy and implantation with iliac bone are often applied to

  19. Magnetic resonance imaging in the evaluation of standard radiotherapy field borders in patients with uterine cervix cancer;Ressonancia magnetica para avaliacao dos limites dos campos classicos de radioterapia em pacientes portadoras de neoplasia maligna de colo uterino

    Energy Technology Data Exchange (ETDEWEB)

    Freire, Geison Moreira; Dias, Rodrigo Souza; Giordani, Adelmo Jose; Segreto, Helena Regina Comodo; Segreto, Roberto Araujo, E-mail: segreto.dmed@epm.b [Universidade Federal de Sao Paulo (UNIFESP-EPM), SP (Brazil). Unit of Radioterapy; Ribalta, Julisa Chamorro Lascasas [Universidade Federal de Sao Paulo (UNIFESP-EPM), SP (Brazil). Dept. de Gynecology

    2010-05-15

    Objective: to evaluate, by means of magnetic resonance imaging, the standardized field borders in radiotherapy for malignant neoplasm of uterine cervix, and to determine the role of this method in the reduction of possible planning errors related to the conventional technique. Materials and methods: magnetic resonance imaging studies for planning of treatment of 51 patients with uterine cervix cancer were retrospectively analyzed. The parameters assessed were the anterior and posterior field borders on sagittal section. Results: The anterior field border was inappropriate in 20 (39.2%) patients and geographic miss was observed in 37.3% of cases in the posterior border. The inappropriateness of both field borders did not correlate with clinical parameters such as patients' age, tumor staging, histological type and degree. Conclusion: the evaluation of standardized field borders with the use of magnetic resonance imaging has demonstrated high indices of inappropriateness of the lateral field borders, as well as the relevant role of magnetic resonance imaging in the radiotherapy planning for patients with uterine cervix cancer with a view to reduce the occurrence of geographic miss of the target volume. (author)

  20. Relationship between Co-expression of Vascular Endothelial Growth Factor and its Receptor, Kinase Insert Domain Containing Receptor and Tumor Angiogenesis in Invasive Carcinoma of the Cervix

    Institute of Scientific and Technical Information of China (English)

    ZhongqingJiang; FengChuanZhu; JunyingQu; XiuZheng; BinZhang; GuizhuWu

    2004-01-01

    OBJECTIVE The aim of the present study was to determine the expression of vascular endothelial growth factor (VEGF) and its receptor, kinase insert domain containing receptor (KDR), and their significance in regulating tumor angiogenesis in the early stages of cervical cancer.METHODS Using the immunohistochemical SP method, the expression of VEGF and KDR was determined in the cancer cells. In addition, the microvessel density (MVD), labeled by CD34 in the tumor stroma, was examined in 18 cases of cervical intraepithelial neoplasms (ClN), 75 cases of early invasive cervix carcinomas (ICC) and 15 specimens of normal cervical epithelium (NCE).RESULTS In ICC cases, VEGF and KDR were mainly expressed in the cellular membrane and/or cytoplasm of tumor cells, while expression of CD34 was found mainly in the vascular epithelial cells of the tumor stroma. The positive expression rate of VEGF and KDR, and the MVD increased remarkably from NCE through CIN to ICC (P<0.01). For the ICC group, in the patients with positive expression of VEGF and KDR, the MVD was significantly higher than those with negative expression of VEGF and KDR (P<0.05). Expression of VEGF in ICC was positively related to KDR expression (r=0.56, P<0.01 ). The MVD was also positively related to both the expression of VEGF (r=0.60, P<0.01), and KDR (r=0.33, P<0.01). In the cases with both positive expression of VEGF and KDR, the MVD was significantly higher than those in which there was negative expression of both (P<0.01 ).CONCLUSION Expression of VEGF and its receptor KDR plays a key role in up-regulating tumor angiogenesis in cervical carcinoma. Co-overexpression of VEGF and KDR results in rapid tumor vasculogenesis. Detection of co-expression of VEGF and KDR may be of value in further understanding tumor angiogenesis and in searching for new targets for anti-angiogenesis therapy in invasive carcinoma of the cervix.

  1. Association of P16-RBSP3 inactivation with phosphorylated RB1 overexpression in basal-parabasal layers of normal cervix unchanged during CACX development.

    Science.gov (United States)

    Chakraborty, Chandraditya; Roychowdhury, Anirban; Samadder, Sudip; Roy, Anup; Mandal, Ranajit Kumar; Basu, Partha; Roychoudhury, Susanta; Panda, Chinmay Kumar

    2016-10-01

    To understand the molecular mechanism of RB1 phosphorylation in basal-parabasal layers of normal cervix and during cervical cancer (CACX) development, we analyzed the alterations (expression/methylation/deletion/mutation) of RB1/phosphorylated RB1 (p-RB1) (ser807/811 and ser567) and two RB1 phosphorylation inhibitors, P16 and RBSP3, in disease-free normal cervical epithelium (n = 9), adjacent normal cervical epithelium of tumors (n = 70), cervical intraepithelial neoplasia (CIN; n = 28), CACX (n = 102) samples and two CACX cell lines. Immunohistochemical analysis revealed high/medium expression of RB1/p-RB1 (ser807/811 and ser567) and low expression of P16 and RBSP3 in proliferating basal-parabasal layers of majority of normal cervical epitheliums, irrespective of HPV16 infection. Interestingly, 35-52% samples showed high/medium expression of P16 in basal-parabasal layers of normal and had significant association with deleterious non-synonimous SNPs of P16. Methylation of P16 and RBSP3 in basal-parabasal layers of normal cervix (32 and 62%, respectively) showed concordance with their respective expressions in basal-parabasal layers. The methylation frequency of P16 and RBSP3 in basal-parabasal layers of normal did not change significantly in CIN and CACX. The deletion frequency of P16 and RB1 increased significantly with CACX progression. While, deletion of RBSP3 was high in CIN and comparable during CACX progression. P16 showed scattered and infrequent mutation in CACX. The alteration of P16 and RBSP3 was synergistic and showed association with overexpression of p-RB1 in tumors and associated with poor prognosis of patients. Thus, our data suggest that overexpression of p-RB1 in basal-parabasal layers of normal cervical epithelium was due to methylation/low functional-linked non-synonimous SNPs of P16 and RBSP3. This pattern was maintained during cervical carcinogenesis by additional deletion/mutation.

  2. Prognostic value of lymph node ratio in patients with small-cell carcinoma of the cervix based on data from a large national registry

    Directory of Open Access Journals (Sweden)

    Zhou J

    2015-12-01

    Full Text Available Juan Zhou,1,* Jia-Yuan Sun,2,* Shan-Yu Chen,3 Feng-Yan Li,2 Huan-Xin Lin,2 San-Gang Wu,3 Zhen-Yu He2 1Department of Obstetrics and Gynecology, Xiamen Cancer Center, The First Affiliated Hospital of Xiamen University, Xiamen, 2Department of Radiation Oncology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangzhou, 3Department of Radiation Oncology, Xiamen Cancer Center, The First Affiliated Hospital of Xiamen University, Xiamen, People’s Republic of China *These authors contributed equally to this work Objective: To investigate the prognostic value of the lymph node ratio (LNR in patients with small-cell carcinoma of the cervix (SCCC after cancer-directed surgery using a population-based national registry (Surveillance Epidemiology and End Results [SEER].Methods: We retrospectively reviewed the data of SCCC patients in the SEER database from 1980 to 2012. The prognostic impact of LNR with respect to cause-specific survival (CSS and overall survival (OS was analyzed.Results: A total of 118 patients with SCCC were identified. The median follow-up was 30.5 months. All these patients were treated with cancer-directed surgery and lymphadenectomy. Sixty (50.8% patients had nodal metastases. The median LNR was 0.16 in patients with positive lymph nodes. Univariate analysis showed that prognostic factors such as International Federation of Gynecology and Obstetrics (FIGO stage, nodal status, LNR, and local treatment modalities affected CSS and OS (P<0.05. Multivariate analysis showed that LNR was an independent prognostic factor for CSS and OS. Patients with a higher LNR had worse CSS (hazard ratio [HR]: 8.832; 95% confidence interval [CI]: 3.762–20.738; P<0.001 and OS (HR: 8.462; 95% CI: 3.613–19.821; P<0.001. LNR was associated with CSS and OS by stage, especially in FIGO stage I–II patients.Conclusion: LNR is an independent prognostic factor in

  3. 基质金属蛋白酶-9与宫颈成熟关系的研究%Relationship of matrix metalloproteinase-9 and ripening of pregnant cervix

    Institute of Scientific and Technical Information of China (English)

    郭建新; 陈竹钦; 李力

    2001-01-01

    Objective To investigate whether matrix metalloproteinase-9 (MMP-9) was synthesized in pregnant cervix during parturition and its source and distribution. Methods Cervical species (n=10, each weighing about 0.3 g) were taken from pregnant women immediately after delivery. Other cervical species (n=7) were served as negative control from those non-pregnant women but undergoing uterotomy due to other benign diseases. Immunohistochemical method (ABC) was carried out to detect the expression of MMP-9, with a monoclonal antibody against MMP-9. Results Positive staining of MMP-9 was found in the cytoplasm of polymorphonuclear leukocytes (PMN) that had infiltrated into cervix or located in blood vessels of cervix. Scattered light positive staining were found in some interstitial cells of the cervix. No other cells including fibrocytes and lymphocytes were positive to MMP-9. No positive staining was found in control tissues. Conclusion There are strong expressions of MMP-9 in pregnant cervix in term labor, derived mainly from infiltrated PMN. MMP-9 may be an important regulator in the process of cervical ripening.%目的 研究分娩时宫颈组织中是否有基质金属蛋白酶-9(MMP-9)的合成,及其来源与分布。方法 以MMP-9单克隆抗体为一抗,用ABC法对分娩期孕妇宫颈组织(n=10)进行免疫组化分析,以非孕期宫颈组织作对照(n=7)。结果 分娩期孕妇宫颈中浸润的多形核白细胞(PMN)胞浆中有MMP-9的阳性染色,宫颈血管中的PMN胞浆中有阳性染色,宫颈组织间质中有散在的弱阳性染色;其它细胞均无阳性染色,对照组中未发现MMP-9的阳性染色。结论 分娩时宫颈组织有MMP-9的合成和分泌,主要来源于宫颈中浸润的PMN;MMP-9可能是宫颈成熟过程中重要的调控因子。

  4. Clinical Effect of Autonomous Uterine Cavity Water Sac Compression for Hemorrhea and Hemabate in Treatment of Postpartum Hemorrhage Deliv-ering by Vagina%自制宫腔水囊压迫止血联合欣母沛治疗阴道分娩产后出血的临床疗效

    Institute of Scientific and Technical Information of China (English)

    卢丹

    2016-01-01

    Objective To observe the clinical curative effect of autonomous uterine cavity water sac compression for hemor-rhea and hemabate in treatment of postpartum hemorrhage caused by uterine inertia delivering by vagina. Methods 132 cases of patients with postpartum hemorrhage caused by uterine inertia delivering by vagina treated from January 2011 to December 2014 were given clinical experimental observation and randomly divided into two groups, the experimental group were treated with autonomous uterine cavity water sac compression for hemorrhea and hemabate, the control group were treated with simple hemabate, and the delivery outcomes were compared between the two groups. Results The incidence rates of complications in the experimental group and the control group were respectively 6.1% and 12.1%, and the inci-dence rates of laparotomy hemostasis in the experimental group and the control group were respectively 1.5% and 7.6%, P<0.05. Conclusion The clinical effect of autonomous uterine cavity water sac compression for hemorrhea and hemabate in treatment of postpartum hemorrhage caused by uterine inertia delivering by vagina is better, and it can effectively stop bleeding and is worth promotion.%目的:探讨自制宫腔水囊压迫止血联合欣母沛治疗阴道分娩宫缩乏力性产后出血的临床疗效。方法整群选取2011年1月—2014年12月该院收治的132例阴道分娩宫缩乏力性产后出血患者进行临床实验观察,随机分为实验组与对照组,实验组使用自制宫腔水囊压迫止血联合欣母沛治疗,对照组使用单药欣母沛治疗,比较两组患者分娩结局。结果两组患者的并发症发生率为6.1%和12.1%,开腹手术止血为1.5%和7.6%(P<0.05)。结论使用自制宫腔水囊压迫止血联合欣母沛治疗阴道分娩宫缩乏力性产后出血具有较好的临床效果,能够有效止血,值得推广。

  5. Metabolism and distribution of elementary silver on nanometer silver antibacteria gel applied the rat vagina%纳米银抗菌水凝胶大鼠阴道给药后体内银元素的代谢与分布

    Institute of Scientific and Technical Information of China (English)

    常淼; 彭秀丽; 张庆峰

    2011-01-01

    目的 研究纳米银抗菌水凝胶阴道给药后银元素在大鼠组织器官中的代谢与分布情况.方法 雌性Wistar大鼠随机分为试验组与对照组,试验组每天阴道给纳米银抗菌水凝胶1次,剂量为1750μg,连续给药5d;对照组不给药.采用原子吸收光谱法测定血清与器官中的银元素.结果 试验组给药后血清银元素浓度逐渐上升,至第5天达到高峰,后呈下降趋势;银元素在大鼠体内的分布情况是:脾>肾>肾上腺>肝>肺>心>子宫和卵巢>脑.结论 纳米银抗菌水凝胶阴道给药后在短时间内会产生一定浓度的蓄积.%Objective To study metabolism and distribution of elementary silver in several tissues of rats after nanometer silver antibacteria gel applied the rat vagina. Methods Pantheress Wistar rats were randomly divided into the test group and the control group. The 1750 μg of gel was administered to vagina once per day, continuously for 5 ds in test group. Don't give a medicine in control group. Adopt the atomic absorption spectrum method to measure the elementary silver content in serum and tissues. Results After the application of nanometer silver antibacteria gel, the serum elementary silver density in the test group increased gradually and attained peak 5th days, then showed a decreased trend. The distribution of elementary silver in the rat body was spleen > kidney > adrenal glands > liver > lung > heart > the womb and ovary > brain. Conclusion The nanometer silver antibacteria gel has a good bio-safety applied in the vagina.

  6. SU-E-P-58: Dosimetric Study of Conventional Intensity-Modulated Radiotherapy and Knowledge-Based Radiation Therapy for Postoperation of Cervix Cancer

    Energy Technology Data Exchange (ETDEWEB)

    Ma, C; Yin, Y [Shandong Tumor Hospital, Jinan, Shandong Provice (China)

    2015-06-15

    Purpose: To compare the dosimetric difference of the target volume and organs at risk(OARs) between conventional intensity-modulated radiotherapy(C-IMRT) and knowledge-based radiation therapy (KBRT) plans for cervix cancer. Methods: 39 patients with cervical cancer after surgery were randomly selected, 20 patient plans were used to create the model, the other 19 cases used for comparative evaluation. All plans were designed in Eclipse system. The prescription dose was 30.6Gy, 17 fractions, OARs dose satisfied to the clinical requirement. A paired t test was used to evaluate the differences of dose-volume histograms (DVH). Results: Comparaed to C-IMRT plan, the KBRT plan target can achieve the similar target dose coverage, D98,D95,D2,HI and CI had no difference (P≥0.05). The dose of rectum, bladder and femoral heads had no significant differences(P≥0.05). The time was used to design treatment plan was significant reduced. Conclusion: This study shows that postoperative radiotherapy of cervical KBRT plans can achieve the similar target and OARs dose, but the shorter designing time.

  7. Development and clinical applications of digitized fluorescence endoscopic imaging system for the detection of early neoplasms in the oral cavity and uterine-cervix

    Science.gov (United States)

    Zheng, Wei; Tay, Sun K.; Soo, K. C.; Olivo, Malini

    2002-05-01

    A digitized fluorescence endoscopic imaging (DFEI) system combined with 5-Aminolevulinic Acid (5-ALA) induced protoporphyrin IX (PPIX) was developed for the detection of neoplasms in the oral cavity and uterine-cervix. The system has the capability of producing both the digital and video fluorescence images in real time, and also quantifying the fluorescence images. The results show that using the DFEI system associated with the fluorescence image quantification method, both high sensitivity and specificity can be achieved during the head and neck, and obstetric and gynaecology clinical trials. The red fluorescence intensity distribution in the lesion area can also be obtained after digital image processing to better understand the situation of PPIX accumulation in the tissues. Furthermore, applying the intensity ratio IR/IB at red and blue wavelength regions of the tissue targeted, where IR includes the intensity of PPIX fluorescence and red tissue autofluorescence, and IB is the intensity of diffusely back-scattered excitation blue light, different histopathological grades of lesions can be classified by the DEFI system, suggesting a significant potential of the noninvasive optical biopsy for the early cancer detection.

  8. Early stage carcinoma of the uterine cervix. Effects of intracavitary radium treatment on lymphoid cells in blood and pelvic lymph nodes

    Energy Technology Data Exchange (ETDEWEB)

    Onsrud, M.; Grahm, I.; Gaudernack, G.

    Sixteen patients with early stage carcinoma of the uterine cervix treated with primary radical hysterectomy and pelvic lymphadenectomy were compared with 17 patients who four to six weeks before the operation received intracavitary treatment with radium. The calculated radiation dose to the pelvic wall was approximately 10 Gy. The distribution of lymphoid cells in blood and pelvic lymph nodes was studied by an indirect immunoflourescence technique using monoclonal antibodies. The radium treated group showed a significant reduction of circulating OKT4+ (T helper) and OKT8+ (T suppressor/cytotoxic) lymphocytes. The number of Leu7+ (natural killer) cells and 1D5+ cells (monocytes) was not changed, but the ratio between monocytes and T cells was increased after radium therapy. In cell suspensions obtained from the pelvic lymph nodes, the radium treatment induced a significant reduction of the OKT4+ cell fraction. It is concluded that this low dose rate regimen of intracavitary treatment induces changes in the immune system which are of the same type as those seen after external field irradiation.

  9. Expression of 3β-hydroxysteroid dehydrogenase in ovarian and uterine tissue during diestrus and open cervix cystic endometrial hyperplasia-pyometra in the bitch.

    Science.gov (United States)

    Gultiken, Nilgun; Yarim, Murat; Yarim, Gul Fatma; Gacar, Ayhan; Mason, James Ian

    2016-07-15

    The purpose of this study was to compare the expression of 3β-hydroxystreroid dehydrogenase (3β-HSD) in the uterus and ovary of healthy dogs and those with cystic endometrial hyperplasia and/or pyometra complex (CEH-pyometra). Eighteen female dogs were included in the study. Eleven bitches with open cervix CEH-pyometra were included in the CEH-pyometra group and seven diestrus bitches in the control group. For immunostaining a rabbit polyclonal, one raised against recombinant human type 2 (adrenal/gonadal) 3β-HSD was used. Progesterone (P4) concentrations were not statistically different between the groups. Strongly stained large interstitial cell groups in the ovarian medulla were observed particularly in CEH-pyometra group although these cells in the control group were weakly or moderately stained and existed singly or paired. The expressions of 3β-HSD in luminal epithelium (42.40 ± 22.40% vs. 18.42 ± 13.15%, P  0.05). Similarly, the significant increase in the expression of 3β-HSD in ovarian interstitial cells (33.86 ± 29.44 vs. 1.13 ± 2.97, P < 0.05) was found in CEH-pyometra group compared to the control group. The study revealed that 3β-HSD expression in the endometrium of canine CEH-pyometra was significantly high.

  10. Sanazole as a sensitizer of hypoxic cells with radical radiation in the treatment of advanced cancer of cervix an Indian experience.

    Science.gov (United States)

    Huilgol, Nagraj G; Dobrowsky, Werner; Tatsuzaki, Hideo; Chatterjee, Neela A; Kagiya, V T; Das, Kaushik

    2002-06-01

    AK-2123, is a nitrotriazole with a potential to sensitize hypoxic tissue to radiation. Cancer of cervix in advanced stages are predominantly treated with radiation. These are the tumours which harbour a large hypoxic core. This is an Indian experience of the multicentric trial. Patients were randomized to control and AK-2123 arm. 49 patients were randomized to each group. Patients received external radiation with telecobalt to a dose of 50 Gy in five weeks. Those in the study arm received 600 mg/m2, on alternate days. The patients were further treated with intracavitory radiation a dose of 20 Gy. The total dose of 70 Gy was achieved. Patients in the study arm had a complete response of 71.43% (35 of 49) while only 21 of 49 (42.86%) responded in the control group. The overall survival at two years was 72.2% for the study group and 32.43% for control. Neuropathy, a drug related toxicity was transient except, in one patient, which has persisted. AK-2123, has shown significant radiation sensitizing potential.

  11. The negative predictive value of p16INK4a to assess the outcome of cervical intraepithelial neoplasia 1 in the uterine cervix

    DEFF Research Database (Denmark)

    Hariri, Jalil; Øster, Anne

    2007-01-01

    The immunohistochemical expression of p16 in formalin-fixed and paraffin-embedded histological sections was evaluated in a retrospective study comprising a low-grade group of 100 cases of cervical intraepithelial neoplasia (CIN) 1, a high-grade group of 50 cases of CIN 2 to 3, and a benign group...... of 50 cases of normal tissue or benign lesions in the uterine cervix. The cases were consecutive within each group and had a minimum follow-up period of 5 years. Positive reaction for p16 was detected in all cases in the high-grade group and in only 3 cases in the benign group. In the low-grade group......, a total of 9 cases had to be excluded. The remaining 91 cases in the low-grade group showed positive reaction for p16 in 65 cases (71%), including 23 cases that progressed to a high-grade lesion, 36 cases that revealed normal cytological and/or histological picture during the follow-up period, and 6 cases...

  12. A rat model for functional characterization of pregnancy-induced denervation and postpartum reinnervation in the myometrium and cervix: a superfusion study.

    Science.gov (United States)

    Zupkó, I; Csonka, D; Falkay, G

    2005-11-01

    The pregnancy-induced rapid degeneration of the adrenergic nerves innervating the uterus is a well-known but poorly understood phenomenon. Since most of the published investigations were carried out by histological assay, our aim was to describe the loss of the adrenergic function during pregnancy and the re-innervational procedure in the postpartum period. Myometrial and cervical samples from rats were loaded with [3H]noradrenaline and then transferred into a chamber for superfusion. After a wash-out period, fractions were collected. The fifth and fifteenth fraction tissues were stimulated with an electric field. The [3H]noradrenaline contents of the fractions were determined, together with the amount remaining in the tissue. The myometrial [3H]noradrenaline release was substantially decreased in early pregnancy, and absent in the late stage. Differences in release profile were detected between the implantation sites and the interimplantation areas. As a refinement of the results of previous histochemical studies, the noradrenergic functions of the cervix were found to be deeply affected in the early postpartum period. The pregnancy-induced denervational procedure can be followed by means of a superfusional technique after [3H]noradrenaline loading. As our technique is considered to be similar in sensitivity to histological methods, superfusion can be regarded as a model for functional investigations of pregnancy-induced denervation.

  13. [Cervix cancer and pregnancy. Experience of 5 years at the Gynecology-Obstetrics Hospital No. 3, C. M. La Raza from the Mexican Institute of Social Security].

    Science.gov (United States)

    Novoa Vargas, A; Padilla Cortez, M E; Bacelis Castaño, C

    1999-12-01

    Of the 300 clinical files of pregnant women, 22 were associated with cancer plus pregnancy in 5 years. Of them 7 were excluded in the work. 15 patients with CaCu and pregnancy were studied in the Gineco-Obstetric Hospital No. 3 C.M. La Raza of the IMSS México City in a period since 1st of January 1988 to 31st December of 1992. Early menarchia and sexual life, take an important roll in the mexican women as predisponent factors, multideliver and tobacco too. The colposcopy with manage cervix biopsy is the election method of diagnosis, with 99.5% of confiability. In the 15 patients, the estirpe was epidermoid cancer in all of them. The diagnosis of pregnancy age made in the first and third trimester of gestation. Only in 3 patients (20%) were founded visible lesion, this rename the necessity of make detection of CaCu during the pregnancy, because we will can found the cases in preinvader stages or early invader, that cure highly, like the cases seen in this work. Stages preinvader and early invaders during the pregnancy are highly curatives. We concluded that they have worst prognosis of survival, aggressivity and early relapse in patients with pregnancy stage by stage. The last because in the Historical Literature review about this topic, there was statistical relevance about pregnancy in the women with CaCu, using our work for reference. CaCu does change his prognosis associated with the pregnancy.

  14. Treatment of Stages I and II cancer of the cervix: analysis of 5 year survival and recurrence rates. [Effects of surgery an incidence of complications following radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Tavares, M.A.; da Conceicao Belo, M.; Santos, M.

    1979-03-01

    Eight hundred and thirty five patients with a diagnosis of Stages I or II carcinoma of the cervix were treated from 2 January 1965 to 30 June 1971. The purpose of this study was to calculate the 5 year survival rates and to analyze the treatment failures according to the modality of treatment applied. Two series of Stages Ib and IIa patients were available; one group was treated with radiotherapy, and the other with radical hysterectomy and pelvic lymphadenectomy after previous intracavitary radiumtherapy. No statistically significant difference was found in the 5 year survival of both groups. Most Stage IIb patients were treated with radiotherapy. When residual tumor was found in the uterus of a patients who underwent radical surgery after intracavitary radiumtherapy it did not influence survival. On the other hand, the presence of metastatic pelvic lymph nodes after intracavitary radium treatment was related to a lowered survival rate. The number of severe injuries was higher in patients who were treated surgically. Recurrences developed within 5 years after completion of treatment in 10.8% of Stage Ib patients, 21.5% of Stage IIa patients, and 34.5% of Stage IIb patients. Ninety per cent of these recurrences appeared within 3 years after therapy.

  15. From a Script to a Movement——Development Course of the Feminism Drama "The Vagina Monologue"%从剧本走向运动——论女性主义先锋剧《阴道独白》的发展历程

    Institute of Scientific and Technical Information of China (English)

    石艺

    2012-01-01

    《阴道独白》不仅是一部女性主义先锋剧,更掀起了一场女性主义运动:作为一部剧本,其"独白"的特色鲜明;作为一种戏剧实践,它兼具先锋性与本土化的特征;作为一场戏剧运动,该戏取得的反响强烈、持久。%"The Vagina Monologues" is not only a feminist vanguard drama,but also set off a feminist movement.It has a distinctive characteristic of monologue as a drama itself and both vanguard and local features as a drama practice.It is also a peculiar drama movement gaining wide resonance and is of great enlightenment value.

  16. Diagnosis and treatment of small cell carcinoma of the cervix%宫颈小细胞癌诊治临床案例分析

    Institute of Scientific and Technical Information of China (English)

    雷磊; 张靖; 王国庆; 梁静; 胡丽娟; 周敏

    2016-01-01

    Objective:To study the diagnosis and treatment of small cell cervical cancer. Methods:The clinical data of 19 cases of small cell carcinoma of the cervix were retrospectively analyzed,including clinical results,imaging findings,pathologic diagnosis,treatment and follow-up results. Results:All 19 cases of small cell cervical carcinoma patients had pathologic specimens of Syn markers positive results,and 73. 68% patients had medical imaging results in vivo tumor morphology cauliflower type,the results were statistically significant. 13 cases were treated with opera-tion,all patients received neoadjuvant chemotherapy( NAC),including 6 patients received radiotherapy,chemothera-py,6 patients had lymph node metastasis,recurrence happened in lungs,and 2 patients had 1 LM,4 patients had 2~6 LM. Conclusion:To conduct a comprehensive inspection on patients with small cell carcinoma of the cervix and judge the condition accurately,choose the appropriate treatment according to the actual situation of patients,can effec-tively prolong the survival period,improve the quality of life of patients.%目的:探讨宫颈小细胞癌临床诊治体会.方法:对19例宫颈小细胞癌患者临床资料进行回顾性分析,内容包括临床影像检查结果、病理诊断结果、治疗方案、治疗效果以及随访结果等.结果:19例宫颈小细胞癌患者中,全部患者病理检查标本均对Syn标记结果呈阳性,73.68%的患者医学影像检查结果可知体内肿瘤形态呈菜花型,对比结果具有统计学意义.13例患者实施手术治疗,所有患者均实施新辅助化疗(NAC),其中6例患者接受了同步放、化疗治疗方案.6例患者治疗后发生淋巴结转移,复发部位大多为肺部,其中2例患者出现1枚淋巴结转移、4例患者出现2~6枚淋巴结转移.结论:对宫颈小细胞癌患者进行全面检查并准确判断病情,根据患者实际情况选择合适的治疗方案,可有效延长患者生存期.

  17. Effects of uterine cervix constriction on Wistar rats Efeitos da constrição do cérvix uterino em ratos Wistar

    Directory of Open Access Journals (Sweden)

    Cassio Eduardo Raposo-Amaral

    2010-12-01

    Full Text Available PURPOSE: To verify if uterine cerclage can induce craniosynostosis or any cranial deformity in new born Wistar rats. METHODS: One pregnant female Wistar rat underwent laparotomy on day 18 of gestation and the uterus cervix was closed with a 3-0 nylon suture to avoid delivery, that occurs normally on the 21 day. The suture was released after 48 hours beyond the normal gestation period. The female rat delivered 11 pups. Six surviving rats from the delivery (group A - constrained group. Two rats were born from another mother and in the same age were used as control group (group B - 2 nonconstrained controls were allowed to grow. They were sacrificed 1.2 years after their birth all the eight animals. Linear measurement, routine histology and computed tomography of the skull were performed at the time of their death to evaluate the cranial asymmetries by mesurements of the anatomical landmarks of the craniofacial skeleton of the rats on the two groups and compared then. RESULTS: We did not observe statistically significant differences in any of the compared measurements (p>0.05 obtained through the morphologic and radiologic methods. Histologic examinations did not reveal any sign of premature fusion or suture imbrications. Critical decrease in longitudinal body size was noticed as the limbs too in all the animals of group A. CONCLUSION: Constriction of uterine cervix leads to fetus suffering, even death for a few animals, associated to small body size, but not to craniosynostosis.OBJETIVO: Verificar se a cerclagem intra-uterina pode induzir, ao nascimento de ratos Wistar, craniossinostose ou qualquer outra deformidade craniana. MÉTODOS: Uma rata Wistar prenhe foi submetida à laparotomia no 18º dia de gestação e o cérvix uterino foi suturado com 3-0 nylon, impedindo o parto normal que normalmente ocorre no 21º dia de gestação. A sutura foi liberada 48 horas após o período gestacional normal. A rata gestante deu à luz 11 animais. Seis

  18. A pilot study of first-line chemotherapy with 5-FU and Platinum in advanced and recurrent cancer of the cervix

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    Ghaemmaghami F

    2003-06-01

    Full Text Available This study was designed to assess the role of first-line chemotherapy with 5-FU and platinum in the treatment of advanced or recurrent cervical cancer, ten patients with advanced or recurrent cancer of the cervix, with no prior chemotherapy were entered in phase II trial, from Oct. 2000 to Nov. 2001. Eight patients were treated with cis-platinum (50 mg/m2 over 60 minutes in first day followed by 5-FU (1 g/m2 over 24 hours for 4 days and two patients with impaired renal function were treated with carboplatin (300 mg/m2 over 15 minutes in first day followed by 5-FU (1 g/m2 over 24 hours for 4 days every three weeks, until progression of disease or prohibitive toxicity had been observed. Median age was 52 years (range: 28-70 years. Ten patients received a total of 42 cycles of chemotherapy. The mean number of chemotherapy cycles was 4.2 (median 4, range: 3-7. Three patients had partial response (30%, CI, 1.7%-58.5%. Mean response duration was 198 days (range: 122-273 days. Four patients required red blood cell transfusions; three of them had grade II and one of them grade III nausea and vomiting Two had fever and neutropenia (one developed acute renal insufficiency, and there wee no treatment related mortalities. First-line chemotherapy with platinum and 5-FU for advanced recurrent cervical cancer is promising and deserves consideration for large phase III trials.

  19. Treatment Outcome of Medium-Dose-Rate Intracavitary Brachytherapy for Carcinoma of the Uterine Cervix: Comparison With Low-Dose-Rate Intracavitary Brachytherapy

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    Kaneyasu, Yuko, E-mail: kaneyasu@hiroshima-u.ac.jp [Department of Radiation Oncology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima (Japan); Department of Radiation Oncology, Tokyo Women' s Medical University, Tokyo (Japan); Kita, Midori [Department of Radiation Oncology, Tokyo Women' s Medical University, Tokyo (Japan); Department of Clinical Radiology, Tokyo Metropolitan Tama Medical Center, Tokyo (Japan); Okawa, Tomohiko [Evaluation and Promotion Center, Utsunomiya Memorial Hospital, Tochigi (Japan); Maebayashi, Katsuya [Department of Radiation Oncology, Tokyo Women' s Medical University, Tokyo (Japan); Kohno, Mari [Department of Diagnostic Imaging and Nuclear Medicine, Tokyo Women' s Medical University Hospital, Tokyo (Japan); Sonoda, Tatsuo; Hirabayashi, Hisae [Department of Radiology, Tokyo Women' s Medical University Hospital, Tokyo (Japan); Nagata, Yasushi [Department of Radiation Oncology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima (Japan); Mitsuhashi, Norio [Department of Radiation Oncology, Tokyo Women' s Medical University, Tokyo (Japan)

    2012-09-01

    Purpose: To evaluate and compare the efficacy of medium-dose-rate (MDR) and low-dose-rate (LDR) intracavitary brachytherapy (ICBT) for uterine cervical cancer. Methods and Materials: We evaluated 419 patients with squamous cell carcinoma of the cervix who were treated by radical radiotherapy with curative intent at Tokyo Women's Medical University from 1969 to 1999. LDR was used from 1969 to 1986, and MDR has been used since July 1987. When compared with LDR, fraction dose was decreased and fraction size was increased (1 or 2 fractions) for MDR to make the total dose of MDR equal to that of LDR. In general, the patients received a total dose of 60 to 70 Gy at Point A with external beam radiotherapy combined with brachytherapy according to the International Federation of Gynecology and Obstetrics stage. In the LDR group, 32 patients had Stage I disease, 81 had Stage II, 182 had Stage III, and 29 had Stage IVA; in the MDR group, 9 patients had Stage I disease, 19 had Stage II, 55 had Stage III, and 12 had Stage IVA. Results: The 5-year overall survival rates for Stages I, II, III, and IVA in the LDR group were 78%, 72%, 55%, and 34%, respectively. In the MDR group, the 5-year overall survival rates were 100%, 68%, 52%, and 42%, respectively. No significant statistical differences were seen between the two groups. The actuarial rates of late complications Grade 2 or greater at 5 years for the rectum, bladder, and small intestine in the LDR group were 11.1%, 5.8%, and 2.0%, respectively. The rates for the MDR group were 11.7%, 4.2%, and 2.6%, respectively, all of which were without statistical differences. Conclusion: These data suggest that MDR ICBT is effective, useful, and equally as good as LDR ICBT in daytime (about 5 hours) treatments of patients with cervical cancer.

  20. A prospective dosimetric and clinical comparison of acute hematological toxicities in three-dimensional conformal radiation therapy and intensity modulated radiation therapy with concurrent chemotherapy in carcinoma cervix

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    H U Avinash

    2015-01-01

    Full Text Available Background and Objectives: Acute hematological toxicities are an important cause of morbidity in patients receiving concurrent chemoradiation to pelvis in carcinoma cervix. The objective of this study was to evaluate the role of intensity-modulated radiotherapy (IMRT in reducing the dose to the bone marrow as compared with three-dimensional conformal radiotherapy (3DCRT and hence its impact on reducing the acute hematological toxicities. Materials and Methods: Eleven consecutive patients treated with IMRT and 12 patients treated with 3DCRT to the whole pelvis along with concurrent chemotherapy were selected. Bone marrow was delineated. V10 Gy, V20 Gy, V95%, and Dmean of bone marrow were recorded. Weekly blood counts were recorded and graded as per Common Terminology Criteria version 4.0 for all the patients. Results: The dose to the bone marrow V20 Gy was 206.78 ± 57.10 cc (75% and 251.70 ± 40.45 cc (91% for IMRT and 3DCRT, respectively (P = 0.04 and V95% was 23.30 ± 8.34% and 46.76 ± 6.71% for IMRT and 3DCRT, respectively (P = 0.001. The grade of toxicities during each week did not show the difference in either arm. However, the total count and Neutrophil counts during the 2nd week showed statistical significance between IMRT and 3DCRT. Conclusion: IMRT significantly reduces the dose to the bone marrow as compared to 3DCRT. The reduction of the dose did not translate into a decrease in acute hematological toxicities. Concurrent platinum-based chemotherapy is the probable cause for the hematological toxicities.

  1. Disturbance of Bcl-2, Bax, Caspase-3, Ki-67 and C-myc expression in acute and subchronic exposure to benzo(a)pyrene in cervix.

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    Gao, Meili; Li, Yongfei; Ji, Xiaoying; Xue, Xiaochang; Chen, Lan; Feng, Guodong; Zhang, Huqin; Wang, Huichun; Shah, Walayat; Hou, Zhanwu; Kong, Yu

    2016-03-01

    Epidemiological studies have demonstrated that cigarette smoking is an important cofactor or an independent risk factor for the development of cervical cancer. Benzo(a)pyrene (BaP) is one of the most potent tobacco smoke carcinogens in tobacco smoke. BaP induced DNA damage and over expression in p53 cervical tissue of mice as demonstrated in our previous study. Here we present the findings of exposure to BaP on the expression of Bcl-2, C-myc, Ki-67, Caspase-3 and Bax genes in mouse cervix. Acute intraperitoneal administration of BaP (12.5, 25, 50, 100mg/kg body weight) to ICR female mice induced a significant increase in Bcl-2, C-myc, Ki-67 mRNA and protein level till 72h except in Bcl-2 at 24h with 12.5, 25, 50mg/kg as well as at 48h with 12.5mg/kg body weight post treatment. A significant increase was also seen in Caspase-3 and Bax mRNA and protein level with peak level at 24h and gradual decrease till 72h, however, the expression of caspase-3 increased while that of Bax decreased with increasing dose of Bap after 24h. In sub chronic intraperitoneal and oral gavage administration of BaP (2.5, 5, 10mg/kg body weight), similar significant increase was observed for all the examined genes as compared to the control and vehicle groups, however the expression of Bax decreased in a dose dependent manner. The findings of this study will help in further understanding the molecular mechanism of BaP induced carcinogenesis of cervical cancer.

  2. Single-nucleotide polymorphisms and haplotypes of membrane type 1-matrix metalloproteinase in susceptibility and clinical significance of squamous cell neoplasia of uterine cervix in Taiwan women.

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    Tee, Yi-Torng; Liu, Yu-Fan; Chang, Jinghua Tsai; Yang, Shun-Fa; Chen, Shiuan-Chih; Han, Chih-Ping; Wang, Po-Hui; Liao, Chiung-Ling

    2012-09-01

    Membrane type 1-matrix metalloproteinase (MT1-MMP) participates in the activity of MMP-2, which correlates with cancer of uterine cervix. Single-nucleotide polymorphisms (SNPs) in promoter and exon of MT1-MMP may influence their binding with transcription factors and gene transcription. To date, no study reports the association of the MT1-MMP polymorphisms with cervical neoplasia. Therefore, we investigated the influence of the MT1-MMP gene polymorphisms on the susceptibility and clinicopathological variables of cervical neoplasia for women in Taiwan. We recruited 72 patients with cervical squamous cell carcinoma and 63 with high-grade dysplasia as 1 subgroup. Meanwhile, 280 control women were included as another subgroup. The SNPs rs1003349 (site -165), rs2236307 (+7096), and rs3751489 (+8153) as well as rs2236302 (site +6727) of MT1-MMP gene were determined by polymerase chain reaction (PCR)-restriction fragment length polymorphism and real-time PCR genotyping, respectively. Then, we correlated these SNPs and haplotypes with the development of cervical neoplasia and cancer clinicopathological variables. We found that women with CC genotype in rs2236307 SNP exhibited a more risk to develop cervical neoplasia as compared with those with wild genotype TT. Haplotypes -165 T, +6727 C, +7096 C, +8153 G or -165 G, +6727 G, +7096 T, and +8153 G and diplotypes including at least 1 type of these haplotypes of MT1-MMP gene showed a higher risk of cervical neoplasia. However, both haplotypes were not significantly correlated with the clinicopathological characteristics of cervical cancer. In conclusion, Taiwan women with variant homozygote CC (+7096) and haplotypes, TCCG and GGTG, of MT1-MMP exhibit more risk in developing cervical neoplasia.

  3. Phase I/II trial of external irradiation plus medium-dose brachytherapy given concurrently to liposomal doxorubicin and cisplatin for advanced uterine cervix carcinoma

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    Varveris, H.; Kachris, S.; Lyraraki, E.; Petineli, E.; Varveris, A.; Fasoulaki, A. [Dept. of Radiotherapy and Oncology, Medical School of Crete Univ., Iraclion Univ. Hospital (Greece); Mazonakis, M.; Tzedakis, A. [Dept. of Medical Physics, Medical School of Crete Univ., Iraclion Univ. Hospital (Greece); Kouloulias, V. [Dept. of Radiotherapy and Oncology, Medical School of Athens Univ. (Greece); Zolindaki, A. [Dept. of Obstetrics and Gynecology, Medical School of Crete Univ., Iraclion Univ. Hospital (Greece); Vlachaki, M. [New York Univ. Medical Center, NY (United States)

    2006-03-15

    Background and Purpose: although the standard of care for patients with locally advanced uterine cervix carcinoma is cisplatin-(CDDP-)based chemotherapy and irradiation (RT), the optimal regimen remains to be elucidated. A phase I/II study was conducted to evaluate the dose limiting toxicity (DLT) and the maximum tolerated dose (MTD) of liposomal doxorubicin (Caelyx) combined with CDDP and RT for cervical cancer. Patients and Methods: 24 patients with stage IIB-IVA were enrolled (Table 1). They all received external RT (up to 50.4 Gy) and two medium-dose rate (MDR) brachytherapy implants (20 Gy each at point A). The Caelyx starting dose of 7 mg/m{sup 2}/week was increased in 5-mg/m{sup 2} increments to two levels. The standard dose of CDDP was 20-25 mg/m{sup 2}/week. Results: concurrent chemoradiation (CCRT) sequelae and the DLTs (grade 3 myelotoxicity and grade 3 proctitis in five patients treated at the 17 mg/m{sup 2}/week Caelyx dose level) are shown in Tables 2, 3, 4, and 5. After a median follow-up time of 17.2 months (range 4-36 months), four patients had died, 15 showed no evidence of progressive disease, and five (20.8%, 95% confidence interval [CI]: 12.5-29.1%) were alive with relapse (Figure 1). There were seven complete (29.1%, 95% CI: 19.8-38.4%) and 17 partial clinical responses (95% CI: 61.1-80.1%). The median progression-free survival was 10.4 months. Causes of death were local regional failure with or without paraaortic node relapse combined with distant metastases (Table 6). Conclusion: The MTD of Caelyx given concurrently with CDDP and RT was determined at the 12 mg/m{sup 2}/week dose level. The above CCRT schema is a well-tolerated regimen, easy to administer in ambulatory patients, and results appear promising. (orig.)

  4. Low-grade squamous intraepithelial lesions of the cervix with marked cytological atypia-clinical follow-up and human papillomavirus genotyping.

    Science.gov (United States)

    Park, Kay; Ellenson, Lora H; Pirog, Edyta C

    2007-10-01

    The majority of low-grade squamous intraepithelial lesions (LSILs) of the cervix regress spontaneously; however, a small proportion of LSILs progress to high-grade squamous intraepithelial lesion (HSIL) if the lesion is not excised. The guidelines for which patients with LSIL should be treated and which may be followed safely are not well established. The goal of this study was to identify a subgroup of patients with LSIL who may require surgical treatment. We hypothesized that patients with LSILs with marked cytological atypia (LSIL-MA) may be at higher risk for subsequent HSIL. In addition, we were interested in whether LSIL-MA was associated with specific human papillomavirus genotypes. Consecutive patients with biopsy diagnosis of LSIL (n = 30) and LSIL-MA (n = 30) were identified. Marked atypia was defined as 5 or more cells with nuclear enlargement of at least 5 times the size of an intermediate cell nucleus or multinucleation with 5 or more nuclei. Patient follow-up was recorded for up to 24 months. Human papillomavirus genotyping was performed using SPF10 PCR and line probe assay. High-grade squamous intraepithelial lesion on follow-up was significantly more common in patients with LSIL-MA (36%) than in patients with LSIL (7%), and negative follow-up was significantly more common in patients with LSIL (50%) than LSIL-MA (23%). Cases of LSIL and LSIL-MA showed similar spectrum of human papillomavirus genotypes. Marked cytological atypia in LSILs identifies a subset of patients with a high rate of HSIL on follow-up. In such patients, an excisional cone biopsy should be strongly considered.

  5. [Dose-dependent effect of locally administered sulprostone gel on serum luteal and placental hormones in cervix priming in the 1st trimester].

    Science.gov (United States)

    Rath, W; Pitzel, L; Hilgers, R; Kuhn, W

    1989-01-01

    In a prospective, randomised study 30 primigravidae were treated with 25 micrograms, 50 mu, or 100 micrograms sulprostone gel in order to soften the cervix prior to first trimester termination of pregnancy. 10 multigravidae received only the gel vehicle tylose. For objective demonstration of the priming effect, the force required for dilatation of the cervical canal was measured in Newton by a special tonometer before prostaglandin (PG) application and before operation. Serum progesterone, 17-beta-estradiol and hP1 levels were determined radioimmunologically prior to PG application and at two-hours intervals until curettage. A sonographic examination for determing the vitality of the pregnancy was performed before PG administration and immediately before the surgical intervention. There were no significant differences in the primig effect between the 50 micrograms and 100 micrograms sulprostone-treated group; the application of 25 micrograms sulprostone was significantly less effective. After 100 micrograms sulprostone gel abortion occurred in 2 patients, 6 women showed a marked decrease in hPl concentrations, progesterone levels were found to be reduced to 31.6-78.7% and 17-beta-estradiol to 10-40% of the initial values before PG application. We found a close time correlation between the occurrence of contraction-induced lower abdominal pain and the fall in hormone concentrations. No abortions occurred in any of the patients treated with 50 micrograms sulprostone gel; in 9 women without clinical symptoms no significant changes of the hormone concentrations were observed. In contrast to the previously published literature our results indicate that effective cervical ripening can be achieved by this method without disturbance of the feto-placental unit and the trophoblast respectively.

  6. Analysis of the Curative Effect of Preoperative Intra-Arterial Infusion Chemoembolization on Stage,IB2-IIB Uterine Cervix Cancer

    Institute of Scientific and Technical Information of China (English)

    Huashu Li; Fuxiang Liu; Guohe Zhou; Zhaoxia Mo

    2008-01-01

    OBJECTIVE To investigate the short-term and long-term therapeutic efficacy of preoperative intra-arterial infusion chemo-embolization on stage IB2-IIB Uterine cervix cancer (UCC). METHODS A total of 143 patients with Stage IB2-lIB UCC were divided into a clinical trial group and a control group. The patients in the clinical trial group (n/=86) were treated with a combined therapy, i.e. preoperative intra-arterial infusion chemo-embolization, surgical therapy and postoperative radiotherapy, and those in the control group(n=57)were given surgical therapy and post-operative radiotherapy. The adverse effects, changes in local lesion and pathological examinations of the cancer, and the state during the surgery were observed after the intra-arterial infusion chemo-embolization. The survival rate and recurrence rate between the two groups were compared. RESUITS The total effective rate of the intra- arterial infusion chemo-embolization on Stage IB2-IIB UCC was 93.02%. The treatment could reduce tumor size, bring about retro-conversions of the clinical stage of the tumors and pathological grade of the cancer cells, and decrease the quantity, of intra-operative blood loss as well as the operating time. It could significantly improve the 5-year survival rate (P<0.05), and reduce the 2 and 5-yeartumor recurrence rates(P<0.05). Moreover, its side effects were little. CONCLUSIOAN Preoperative intra-arterial infusion chemo-embolization can create conditions for radical operation, lower the postoperative recurrence rate, and improve the prognosis in the patients with UCC. It is an effctive therapy in treating UCC.

  7. Dose variation due to change in planned position for patients with carcinoma of the cervix undergoing high-dose-rate brachytherapy- 2D dose analysis

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    Anil Talluri

    2015-03-01

    Full Text Available Purpose: To assess the dosimetry to organs at risk (OARs in lithotomy position with a planned time-dose pattern obtained from supine position. Methods: The sample consists of thirty patients with carcinoma of the uterine cervix, Stage II and III. Patients often feel discomfort in supine position (S position when compared to lithotomy position (M position due to relaxation of pelvic floor muscles after the insertion of applicator (tandem and ovoids or before delivery of the treatment. Each patient was imaged with orthogonal X- ray radiographs simultaneously in two positions, i.e. S position and M position. Dwell time and dwell position pattern obtained from the optimized plan in S position was used to generate plan in M position. Following dose reference points (point A, pelvic wall points, bladder points, rectal, anorectum (AR point and rectosigmoid (RS point points were identified for analysis in S and M positions. The dosimetric data for reference points generated by the Brachyvision TPS was analyzed.Results: Pelvic wall points registered lower doses in M position when compared to S position. Mean doses for right pelvic wall point (RPW and left pelvic wall point (LPW were reduced by -10.02 % and -11.5% in M position, respectively. International Commission on Radiation Units and Measurements (ICRU bladder point also registered lower doses in M position with a mean dose of -6.8%. Rectal point showed dose reduction by mean of -6.4%. AR and RS points showed an increased dose in M position by a mean of 16.5% and 10%, respectively. Conclusion: Current dosimetry procedure serves as a model with time-dose pattern planned for S position, but delivered in M position, without dose optimization. Prioritization of comfort and position can be considered in conjunction with optimization of dose. 

  8. The Clinical Significance of Digital Examination-Indicated Cerclage in Women with a Dilated Cervix at 14 0/7 - 29 6/7 Weeks

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    Hyun Sun Ko, Yun Seong Jo, Ki Cheol Kil, Ha Kyun Chang, Yong-Gyu Park, In Yang Park, Guisera Lee, Sajin Kim, Jong Chul Shin

    2011-01-01

    Full Text Available Objective. This study was to compare pregnancy outcomes between cerclage and expectant management in wemen with a dilated cervix. Design. Retrospective multicenter cohort study. Setting. Five hospitals of Catholic University Medical Center Network in Korea. Population. A total of 173 women between 14 0/7 and 29 6/7 weeks' gestation with cervical dilation of 1 cm or greater by digital examination. Methods. Pregnancy outcomes were compared according to cerclage or expectant management, with the use of propensity-score matching. Main Outcome Measures. Primary outcome was time from presentation until delivery (weeks. Secondary outcomes were gestational age at delivery, neonatal survival, morbidity, preterm birth, and so on.Results. Of 173 women, 116 received a cerclage (cerclage group, and 57 were managed expectantly without cerclage (expectant group. Cervical dilation at presentation, and the use of amniocentesis performed to exclude subclinical chorioamnionitis differed between two groups. In the overall matched cohort, there was significant difference in the time from presentation until delivery (cerclage vs. expectant group, 10.6±6.2 vs. 2.9±3.2 weeks, p <0.0001. While there was no significant difference in the neonatal survival between two groups, there werelower neonatal morbidity as well as higher pregnancy maintenance rate at 28, 32, 34 and 37 weeks' gestation in the cerclage group, compared with the expectant group.Conclusion. This study suggests that digital examination-indicated cerclage appears to prolong gestation and decrease neonatal morbidity, compared with expectant management in women with cervical dilation between 14 0/7 and 29 6/7 weeks.

  9. Dose Effect Relationship for Late Side Effects of the Rectum and Urinary Bladder in Magnetic Resonance Image-Guided Adaptive Cervix Cancer Brachytherapy

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    Georg, Petra, E-mail: petra.georg@akhwien.at [Department of Radiotherapy, Medical University of Vienna, Vienna (Austria); Poetter, Richard; Georg, Dietmar; Lang, Stefan; Dimopoulos, Johannes C.A.; Sturdza, Alina E.; Berger, Daniel; Kirisits, Christian [Department of Radiotherapy, Medical University of Vienna, Vienna (Austria); Doerr, Wolfgang [Department of Radiotherapy, Medical University of Vienna, Vienna (Austria); Department of Radiotherapy and Radiation Oncology, Medical Faculty Carl Gustav Carus, University of Technology Dresden, Dresden (Germany)

    2012-02-01

    Purpose: To establish dose-response relationships for late side effects of the rectum and bladder in cervix cancer patients after magnetic resonance image-guided adaptive brachytherapy (IGABT). Methods and Materials: A cohort of 141 patients was treated with 45 to 50.4 Gy with or without cisplatin plus 4 fractions of 7 Gy IGABT. Doses for the most exposed 2, 1, and 0.1-cm{sup 3} (D{sub 2cc}, D{sub 1cc}, D{sub 0.1cc}) volumes of the rectum and bladder were converted into the equivalent dose in 2 Gy fractions (EQD2), using a linear quadratic model ({alpha}/{beta} = 3 Gy). Late side effects were prospectively assessed (using late effects in normal tissues subjective, objective, management and analytic [LENT SOMA]) scales. Dose-response relationships were determined by logit analyses. Results: Eleven patients developed rectal side effects, and 23 patients had urinary side effects. A significant dose effect was found for all rectal dose-volume histogram (DVH) parameters for patients with side effect grades of 1 to 4 but was only significant for D{sub 2cc} and D{sub 1cc} for grades {>=}2. The ED10 values for D{sub 2cc} were 73 Gy for grades 1 to 4 and 78 Gy for grades 2 to 4 rectal morbidity. For bladder side effects, a significant dose effect was shown for all DVH parameters for complication grades {>=}2; the respective ED10 was 101 Gy. Conclusions: Well-defined dose-response curves could be established for D{sub 2cc} in the rectum and the urinary bladder.

  10. Comparison of CT based-CTV plan and CT based-ICRU38 plan in brachytherapy planning of uterine cervix cancer

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    Cho, Jung Keun; Han, Tae Jong [Jeonju Univ., Jeonju (Korea, Republic of)

    2007-09-15

    Purpose : In spite of recent remarkable improvement of diagnostic imaging modalities such as CT, MRI, and PET and radiation therapy planing systems, ICR plan of uterine cervix cancer, based on recommendation of ICRU38(2D film-based) such as point A, is still used widely. A 3-dimensional ICR plan based on CT image provides Dose-Volume Histogram(DVH) information of the tumor and normal tissue. In this study, we compared tumor-dose, rectal-dose and bladder-dose through an analysis of DVH between CTV plan and ICRU38 plan based on CT image. Method and Material : We analyzed 11 patients with a cervix cancer who received the ICR of Ir-192 HDR. After 40Gy of external beam radiation therapy, ICR plan was established using PLATO(Nucletron) v.14.2 planning system. CT scan was done to all the patients using CT-simulator(Ultra Z, Philips). We contoured CTV, rectum and bladder on the CT image and established CTV plan which delivers the 100% dose to CTV and ICRU plan which delivers the 100% dose to the point A. Result : The volume(average{+-}SD) of CTV, rectum and bladder in all of 11 patients is 21.8{+-}6.6cm{sup 3}, 60.9{+-}25.0cm{sup 3}, 111.6{+-}40.1cm{sup 3} respectively. The volume covered by 100% isodose curve is 126.7{+-}18.9cm{sup 3} in ICRU plan and 98.2{+-}74.5cm{sup 3} in CTV plan(p=0.0001), respectively. In (On) ICRU planning 22.0cm{sup 3} of CTV volume was not covered by 100% isodose curve in one patient whose residual tumor size is greater than 4cm, while more than 100% dose was irradiated unnecessarily to the normal organ of 62.2{+-}4.8cm{sup 3} other than the tumor in the remaining 10 patients with a residual tumor less than 4cm in size. Bladder dose recommended by ICRU 38 was 90.1{+-}21.3% and 68.7{+-}26.6% in ICRU plan and in CTV plan respectively(p=0.001) while rectal dose recommended by ICRU 38 was 86.4{+-}18.3% and 76.9{+-}15.6% in ICRU plan and in CTV plan, respectively(p=0.08). Bladder and rectum maximum dose was 137.2{+-}50.1%, 101.1{+-}41.8% in ICRU plan

  11. Estadiamento cirúrgico do câncer de colo de útero localmente avançado Surgical staging of locally advanced uterine cervix cancer

    Directory of Open Access Journals (Sweden)

    Heitor Ricardo Cosiski Marana

    2005-12-01

    controle locorregional da doença na pelve.PURPOSE: to assess to what extent the surgical staging differs from the clinical staging among cases of advanced uterine cervix carcinoma, and also to assess the percentage of cases with positive para-aortic ganglia in this group of patients. METHODS: this is a descriptive prospective study in which 36 patients with histological diagnosis of uterine cervix carcinoma considered locally advanced were included (stages IB2, IIB, IIIA and B, and IVA. The cases were submitted to clinical staging, according to FIGO criteria. All patients were to be treated with neoadjuvant chemotherapy. Age ranged from 40 to 73 years, with a mean of 56.2±7.9. The procedure started with pelvic lymphadenectomy followed by para-aortic lymphadenectomy, in case the pelvic lymph nodes were positive on surgical examination. Examination of the abdominal cavity and lymphadenectomy were done either through laparotomy or laparoscopy, chosen at random. In each case, the clinical staging was compared to the surgical staging, considered the gold standard. RESULTS: in the clinical staging (CS, 7 cases were classified as IB2 (tumors larger than 4 cm, 22 cases as CSII and 7 cases as CSIII. The surgical assessment changed the clinical staging as follows: the stage was decreased in six cases, and increased in 13. There was agreement only in 18 cases (50%. The para-aortic lymph nodes were affected in six cases. CONCLUSIONS: clinical staging of locally advanced uterine cervix carcinoma is incorrect in most of the cases. Such inconsistency may lead to excessive treatment in some cases, but about one fourth of the patients with positive para-aortic ganglia would not be adequately treated with the current standard treatment radiotherapy with chemosensitization, which aims at the local regional control of the pelvic disease.

  12. Association of genetic polymorphism of the DNA base excision repair gene (APE-1 Asp/148 Glu) and HPV type (16/18) with the risk of cervix cancer in north Indian population.

    Science.gov (United States)

    Shekari, Mohammad; Sobti, Ranbir Chander; Tamandani, Dor Mohammad Kordi; Malekzadeh, Keyanoosh; Kaur, Pushpinder; Suri, Vanita

    2008-01-01

    Cervical cancer is one of the most common neoplastic diseases affecting women, with a combined world wide incidence of almost half a million new cases. Reduced DNA repair capacity (DRC) can render a high risk of developing many types of cancer; including cervical cancer. Polymorphisms in DNA repair genes may contribute the genetic instability and carcinogenesis. Smoking experience and use of oral contraceptives have been confirmed to be risk factors for cervical cancer. The purpose of the present study was, therefore to investigate APE-1 genotypes (Asp/Asp, Asp/Glu, Glu/Glu) with different histological subtypes in cases compared with controls. It has been observed that Asp/Glu with Glu/Glu genotypes that combined we observed statistically significant with protective effect for developing of cervix cancer (OR-0.51, 95% CI 0.31-0.83, p-0.006). The combined Asp/Glu with Glu/Glu genotypes who were using oral contraceptives were shown to be statistically significant with reduced risk of cervical cancer (OR-0.22 95% CI- 0.11-0.47, p-0.0002). It has been suggested that significantly correlation between HPV 16 and users of oral contraceptives in certain APE-1 genotypes with reduced risk in developing cervix cancer. In conclusion we observed statistical significant association with reduced risk of cervix cancer in APE-1 with different genotypes, though, on the other hand, in association between HPV type 18 and those having SCC, highly increased risk of cervical cancer was observed.

  13. Dietary carotenoids in normal and pathological tissues of corpus uteri.

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    Sławomir Wołczyński

    2008-12-01

    Full Text Available Carotenoids and retinyl esters are the source of vitamin A in the human body and its natural derivatives takes part in the regulation of cell replication and differentiation in the human endometrium, may induce the leiomyoma growth and has a role in differentiation of endometrial adenocarcinoma. The aim of the study was to demonstrate the presence of carotenoids in tissues from the normal uterus and from various tumors of the uterine corpus, as well as to compare the total content, major carotenoids and % of carotenoids belonging to the provitamin A group between the tissues examined. Using three independent methods of chromatography (CC, TLC, HPLC we analysed 140 human samples. We identified 13 carotenoids belonging to the eg. provitamin A group and epoxy carotenoids. In all the samples beta-carotene, beta-cryptoxanthin, lutein, neoxanthin, violaxanthin and mutatoxanthin were isolated. In normal tissues, the mean carotenoid content was the highest in the follicular phase endometrium (9.9 microg/g, while the highest percentage of carotenoids belonging to provitamin A group was found in the luteal phase (18.2%. In the pathological group, the highest mean values were demonstrated for epithelial lesions (8.0 microg/g, and within this group - in endometrioid adenocarcinoma (10.8 microg/g. In both groups, violaxanthin, beta-cryptoxanthin, lutein epoxide and mutatoxanthin were the predominant carotenoids. We have demonstrated that all uterine tissues show a concentration of beta-carotene and beta-cryptoxanthin, being the source of vitamin A. The highest total values of carotenoids obtained in the group of endometrioid adenocarcinoma seem to confirm certain enzymatic defects in carotenoid metabolism in the course of the neoplastic process or some metabolic modifications. The finding of astaxanthin - the major antioxidant among carotenoids - in 63% of tissues examined is also significant.

  14. 分析阴道镜与LEEP治疗宫颈癌前病变的临床有效性%linical Efficacy Analysis of Gynecatoptron and LEEP in the Treatment of Precancerous Lesions of Uterine Cervix

    Institute of Scientific and Technical Information of China (English)

    李媛媛

    2015-01-01

    Objective To analyze the effect of gynecatoptron and LEEP knife in the treatment of precancerous lesions of u-terine cervix in clinic. Methods 48 cases of patients diagnosed as cervical precancerous lesions by colposcopy biopsy pathologic examination were selected from 108 cases of vaginitis patients treated in our hospital from March 2015 to August 2015 and they were given LEEP operation by colposcopy, the clinical treatment effect was analyzed. Results by the cervix biopsy, the sensitivity ,specificity, positive predictive values, negative predictive values , misdiagnosis rate and missed diag-nosis rate of HSIL were respectively 80.83%, 91.43%, 76.38%, 92.29%, 6.9% and 10.4%. and 82.45%、90.06%、77.44%、92.54%、5.3% and 10.5% of LSIL successively, the total coincidence probability of cervix biopsy results and pathological tissue diagnosis of LEEP operation was 95.8%, the rate of conversion was 93.75% after 3 months of follow-up and 100%after 6 months of follow-up by LEEP knife treatment. Conclusion The choice of gynecatoptron and LEEP operation in the treatment of patients with precancerous lesions of uterine cervix can greatly reduce trauma and avoid severe bleeding prob-lems, the application effect of which is obvious.%目的:分析分析阴道镜与LEEP治疗宫颈癌前病变的临床有效性。方法从2015年3—8月该院108例阴道镜患者中,整群选择48例经阴道镜活检病理检查诊断为宫颈癌前病变的入院者,给予阴道镜下LEEP手术治疗,分析其临床治疗效果。结果阴道镜的活检结果和LEEP手术的病理组织诊断的总符合概率95.8%;采用LEEP刀治疗,3个月的随访的转阴率为93.75%,6个月转阴率100%。结论阴道镜与LEEP治疗宫颈癌前病变的临床效果显著。

  15. 子宫颈微偏腺癌1例报道及文献回顾%Minimal deviation adenocarcinoma of the cervix:case report and literature review

    Institute of Scientific and Technical Information of China (English)

    刘小艳; 巩丽; 李艳红; 姚丽; 封兰兰; 兰淼; 张伟

    2013-01-01

    目的:报道1例子宫颈微偏腺癌病例,探讨其临床病理学特征.方法:对1例子宫颈微偏腺癌进行临床病理学及免疫组化观察.结果:显微镜下,腺体由分泌黏液的柱状上皮构成,腺体扭曲,外形不规则,大多数腺体与正常腺体无法区别,少部分腺体的细胞核有中度不典型增生,核分裂象可见.结论:子宫颈微偏腺癌是一种少见的高分化黏液腺癌,临床诊断应排除良性腺体增生及内膜异位.因大多数病例子宫颈活检不能诊断,所以在日常临床工作中如遇到考虑恶性肿瘤,而镜检为良性时,应考虑子宫颈微偏腺癌,腺体浸润深度是诊断的关键.%Objective: To report a case of minimal deviation adenocarcinoma of the cervix, and explore its clinical and pathological features. Methods: The histopathological and immunohistochemical findings of a case of minimal deviation adenocarcinoma of the cervix were observed. Results: Microscopically, glandular organ was set up by mucilaginous of the columnar epithelium, glandular organ squirmed, irregular shape, most of the glandular organs can not distinguish between the normal glandular organ, occasionally, some glandular organs' nucleus had midrange atypical hy-perplasia, infiltration depth of the glandular organ is the key for diagnosis. Conclusion; Minimal deviation adenocarcinoma of the cervix is a rare well - differentiated mucinous adenocarcinoma. Clinical diagnosis should rule out ectopic benign glandular hyperplasia and adenomyosis. Because the majority of cases of cervical biopsy can not be diagnosed, in routine clinical work, clinical considerations in case of malignancy, while the microscopic examination as benign should be considered minimal deviation adenocarcinoma of the cervix.

  16. Local expression of surfactant protein A in the vagina of the patients with RVVC before and after treatment%表面活性蛋白A在复发性外阴阴道假丝酵母菌病患者治疗前后阴道局部的表达

    Institute of Scientific and Technical Information of China (English)

    杨兰英; 贾俊茹; 王素芬; 李凤文

    2013-01-01

    Objective To observe the change of surfactant protein A(SP-A) level from the local vagina of the patients with recurrent vulvovaginal candidiasis (RVVC), discuss the relationship of the development and lapsing between SP-A and RVVC and analyze the effect of He-Ne laser (He -Ne) irradiation as the maintenance therapy on vaginal SP-A. Methods After initial antifungal treatment in the acute phase and adopting the maintenance therapy with He-Ne laser treatment for two courses for RWC patients (observation group), the treatment effect were followed up and the SP-A level of vaginal secretion were determined with ELISA method. At the same time, SP-A of the vaginal secretion specimen from healthy women of child-bearing age were detected and taken as control group. Results SP-A was (7. 24 ± 0. 76) nmol/L in control group with (1. 95 ± 0. 72) nmol/L in the acute phase of RVVC. After initial treatment, SP-A reached (2.92 ± 0. 53) nmol/ L, which was elevated comparing with that of acute episode (P<0.05). SP-A was (3. 64 ± 0. 62) nmol/L after the first treatment course with He-Ne laser, which got significant elevation comparing with that before laser treatment (P<0.05). After the second treatment course with laser, SP-A was (4.27 ±0.56) nmol/L, which was elevated comparing with that of the first treatment course (P< 0.05). On re-examination sixth months after stopping treatment, there was no significant difference for (3. 91 ± 0. 35) nmol/L of SP-A from local vagina in the observation group comparing with that of the laser treatment group after the second treatment course, but it was lower than that of the control group (P<0.001). There was significant decrease in SP-A from each group at various stages of RWC process comparing with that of the control group (P<0.001). Conclusion SP-A exists in local vagina, and the reduction of SP-A may be related to the development of RWC. The local irradiation with He-Ne laser may elevate SP-A level in vagina, thus, it provides evidence

  17. A quality indicator to evaluate high-dose-rate intracavitary brachytherapy for cancer of the cervix; Determinacao de um indicador de qualidade para avaliar a braquiterapia intracavitaria com alta taxa de dose no cancer do colo uterino

    Energy Technology Data Exchange (ETDEWEB)

    Morales, Francisco Contreras; Soboll, Daniel Scheidegger [Hospital A.C. Camargo, Sao Paulo, SP (Brazil). Dept. de Radioterapia. Servico de Fisica Medica

    2000-12-01

    The aim of this report is to prevent a simple quality indicator (QI) that can be promptly used to evaluate the high-dose-rate (HDR) intracavitary brachytherapy for the treatment of cancer of the cervix, and if necessary, to correct applicators' geometry before starting the treatment. We selected 51 HDR intracavitary applications of brachytherapy of patients with carcinoma of the cervix treated with 60 mm uterine tandem and small Fletcher colpostat, according to the Manchester method (dose prescription on point A). A QI was defined as the ratio between the volume of 100% isodose curve of the study insertion and the volume of the 100% isodose curve of an insertion considered to be ideal. The data obtained were distributed in three groups: the group with tandem placement slippage (67,5%), a group with colpostat placement slippage (21,9%), and a third group, considered normal (10,6%). Each group showed particular characteristics (p < 0.0001). QI can be the best auxiliary method to establish the error tolerance (%) allowed for HDR intracavitary brachytherapy. (author)

  18. Positron emission tomography in the management of cervix cancer patients; Tomographie par emission de positons dans la prise en charge des cancers du col de l'uterus

    Energy Technology Data Exchange (ETDEWEB)

    Bonardel, G.; Gontier, E.; Soret, M.; Dechaud, C.; Fayolle, M.; Foehrenbach, H. [Hopital d' Instruction des Armees du Val-de-Grace, Service de Medecine Nucleaire, 75 - Paris (France); Chargari, C.; Bauduceau, O. [Hopital d' Instruction des Armees du Val-de-Grace, Service de Radiotherapie, 75 - Paris (France)

    2009-10-15

    Since its introduction in clinical practice in the 1990 s, positron emission tomography (PET), usually with {sup 18}F-fluoro-2-deoxy-D-glucose ({sup 18}F-F.D.G.), has become an important imaging modality in patients with cancer. For cervix carcinoma, F.D.G.-PET is significantly more accurate than computed tomography (CT) and is recommended for loco-regional lymph node and extra pelvic staging. The metabolic dimension of the technique provides additional prognostic information. Ongoing studies now concentrate on more advanced clinical applications, such as the planning of radiotherapy, the response evaluation after the induction of therapy, the early detection of recurrence. Technical innovations, such as PET cameras with better spatial resolution and hybrid positron emission tomography/computed tomography (PET-CT), available now on the whole territory, provide both anatomic and metabolic information in the same procedure. From the point of view of biological metabolism, new radiopharmaceutical probes are being developed. Those hold promise for future refinements in this field. This article reviews the current applications of F.D.G.-PET in patients with cervix cancer. (authors)