WorldWideScience

Sample records for cervical abrasion lesions

  1. Cervical syphilitic lesions mimicking cervical cancer: a rare case report

    Directory of Open Access Journals (Sweden)

    Xiaoqing Zhu

    2015-02-01

    Full Text Available A woman presented to the hospital due to postcoital vaginal bleeding. The patient was initially diagnosed with cervical carcinoma by clinicians at a local hospital. However, a biopsy of the cervical lesions revealed chronic inflammation and erosion of the cervical mucosa, and the rapid plasma reagin ratio titer was 1:256. The patient was eventually diagnosed with syphilitic cervicitis and treated with minocycline 0.1 g twice a day. The patient was cured with this treatment.

  2. 两种排龈方法在楔状缺损修复中的应用研究%Study of two gingival retraction techniques used in restoring cervical abrasion lesions

    Institute of Scientific and Technical Information of China (English)

    王聪

    2012-01-01

    Objective To investigate the influence of two gingival retraction techniques on the periodontium and clinical performance before the restoration of cervical lesion. Methods 150 teeth with cervical lesion of 50 patients were selected. The teeth were divided into 3 groups randomly. In the experiment group, a conventional retraction cord ( Ultrapaks) and a paste-like material (Expasyls) were applied on the buccal aspects of each subject. In the control group, no gingival retraction techniques was applied before restoration and filled directly. Bleeding on probing and the pain were assessed after application. The retention, marginal adaptation, the presence of secondary caries and the GI after one year were evaluated . Data were analysed using Chi-Square and Kruskal - Wallis tests (a = 0. 05). Results There were significant difference between the rates of subjective feeling of the Ultrapaks and Expasyls. Bleeding was only induced by Ultrapaks in five subjects. After one year, the differences between the experimental and control groups in GI and marginal adaptation were of statistical significance ( P < 0.05 ). While there were no significant difference between the two control groups. The retention of the restoration and the presence of secondary caries were not statistically significant among the 3 groups. Conclusion Utilization of gingival retraction techniques before the restoration of cervical lesions can efficiently improve the quality of the restoration and the two gingival retraction methods were not statistically significant. The cordless gingival retraction technique is more easy, feasible, rapid than the retraction cord.%目的 研究在楔状缺损修复前使用两种排龈方法对其牙周组织及充填效果的影响.方法 选择50例全口至少有3个楔状缺损位于龈缘或龈下的患者(150颗患牙),每例患者的患牙随机分为3组,对照组直接树脂修复,实验组分别用ULTRA排龈线,Expusyl排龈膏排龈后再充填.分别

  3. Os Odontoideum: Rare Cervical Lesion

    Directory of Open Access Journals (Sweden)

    Kristie A Robson

    2011-05-01

    Full Text Available We report the case of a 22-year-old Marine who presented to the emergency department, after a martial arts exercise, with transient weakness and numbness in all extremities. Computed tomography cervical spine radiographs revealed os odontoideum. Lateral flexion–extension radiographs identified atlanto-axillary instability. This abnormality is rare and can be career ending for military members who do not undergo surgical fusion. [West J Emerg Med. 2011;12(4:520–522.

  4. Photodynamic therapy for cervical lesions

    Directory of Open Access Journals (Sweden)

    E. V. Grebenkina

    2014-01-01

    Full Text Available The experience of treatment for precancer and early cervical cancer by photodynamic therapy in 12 patients with primary diagnosis H-SIL (CIN II–III and cancer in situ is described. Chlo-rine photosensitizer Photolon was given intravenously at a dose of 0.75–1.15 mg/kg body weight. 2.5 h later the treatment with polyposition laser exposure (light dose – 150 J/cm2, light power density – 400–500 mW/cm2 was made. Thirty days later conization of the cervix with endocervical curettage assessing therapeutic response of cervical tumor tissue was per-formed. According to histological data complete response was in 4 patients, minute foci of CIN I were determined in 7 patients, 1 patient had foci of CIN II. 8 of 10 HPV-positive patients had complete eradication of HPV after treatment. There were no serious adverse events after light exposure. Marked therapeutic response, high anti-viral activity and good feasibility allow to consider photodynamic therapy as alternative organ-sparing treatment of early cancer and pre-cancer of cervix. 

  5. Diaphragm paralysis from cervical disc lesions.

    Science.gov (United States)

    Cloward, R B

    1988-01-01

    An opera singer, who "made her living with her diaphragm", developed a post-traumatic unilateral radiculopathy due to cervical disc lesions, C3 to C6. During one year of severe neck and left arm pain she gradually lost the ability to sing difficult operatic passages which brought an end to her music career. Following a three level anterior cervical decompression and fusion, the neck and arm pain was immediately relieved. One week later her voice and singing ability returned to its full strength and power permitting her to resume her activities as a vocalist. The diagnosis of paresis of the left hemi-diaphragm as part of the cervical disc syndrome was implied by postoperative retrospective inference.

  6. Analysis of human serum from women affected by cervical lesions.

    Science.gov (United States)

    Barba de la Rosa, Ana P; Lugo-Melchor, Ofelia Y; Briones-Cerecero, Erika P; Chagolla-López, Alicia; De León-Rodríguez, Antonio; Santos, Leticia; Vázquez-Ortiz, Guelaguetza; Salcedo, Mauricio

    2008-01-01

    Cervical cancer is one of the first causes of death in Mexican women population. The plasma proteome has a wide dynamic range concentrations of different protein and their alterations reflect the physiological state of the individual's health. The aim of this study was to characterize the 2D-PAGE serum patterns from healthy women and with different levels of cervical lesions. Changes in haptoglobin, apolipoproteins, and transthyretin, when comparing the serum from healthy women and serum from patients with different levels of cervical lesion were found. The Western blot analysis showed increasing concentrations of metalloproteinases (MMP's), proteins with important biological roles in tumor development and metastasis. Protein profiles in conjunction with MS, bioinformatics, and Western blot analysis, allow us to compile information for the acquisition of results to proposed candidates biomarkers of cervical cancer among Mexican women population.

  7. TTV and HPV co-infection in cervical smears of patients with cervical lesions

    Directory of Open Access Journals (Sweden)

    Tachezy Ruth

    2009-07-01

    Full Text Available Abstract Background The female lower genital tract is a gateway for pathogens entering the host through the mucous membrane. One of the prevalent human viruses is Torque teno virus (TTV. The major reported routes of TTV transmission are fecal-oral and parenteral. Furthermore, other modes of transmission, e.g. sexual contact, are suggested. To investigate the sexual route of TTV transmission, cervical smears of healthy women and those with cervical lesions were screened for the presence of TTV DNA. Methods TTV DNA was studied in cervical smears of 95 patients with cervical lesions and 55 healthy women. Paired serum samples were available from 55 and 42 women, respectively. All healthy women had normal cytology while 44 patients had histologically confirmed low-grade lesion (LGL and 51 high-grade lesion (HGL. TTV DNA was detected with primers specific for the non-coding region. In 40 paired cervical smears and serum samples, the phylogenetic group of TTV isolates was determined. The presence of HPV DNA in cervical smears was detected by means of PCR with MY09/11 primers. Results The prevalence of TTV DNA in cervical smears of healthy women was 52.7% and was comparable with that in paired serum samples (50%. Symptomatic women had significantly higher prevalence of TTV DNA in cervical smears (74.7% than healthy controls. The TTV DNA prevalence in patient serum samples was 51%. The phylogenetic groups of TTV serum isolates were concordant with those of TTV from cervical smears of the same subjects. In cervical smears, a wider variety of TTV isolates was found. The viral loads in cervical smears were 10 to 1000 times as high as in sera. The HPV-positive study subjects had significantly higher TTV DNA prevalence than HPV negatives. The prevalence of TTV was not associated with disease severity. Conclusion High prevalence of TTV in cervical smears suggests that sexual transmission is another mode of expansion of TTV infection among the population. The

  8. Sialyl Lewis x expression in cervical scrapes of premalignant lesions

    Indian Academy of Sciences (India)

    Noé Velázquez-Márquez; Gerardo Santos López; Lucio Jiménez Aranda; Julio Reyes Leyva; Verónica Vallejo Ruiz

    2012-12-01

    Sialylated oligosaccharides of glycoproteins and glycolipids have been implicated in tumour progression and metastases. Altered expression of glycosidic antigens has been reported in cervical cancer. In cervix premalignant lesions, an increased expression of sialic acid has been reported. In the present study we determined the expression profiles of the glycosidic antigens Tn, sialyl Tn (sTn), Lewis a (Lea), sialyl Lewis a (sLea), Lewis x (Lex) and sialyl Lewis x (sLex) in cervical scrapes with cytological diagnoses of normal, low-grade squamous intraepithelial lesions (LGSIL) and high-grade squamous intraepithelial lesions (HGSIL). Cervical scrapings were collected to detect tumour antigens expressions by flow cytometry using monoclonal antibodies. Cytometry analysis of Tn, sTn, Lea and Lex did not reveal differences at the expression level among groups. The number of positive cells to sLea antigen increased in the HGSIL group with respect to the normal group (=0.0495). The number of positive cells to sLex antigen in the samples increased with respect to the grade of squamous intraepithelial lesion (SIL) ( < 0.001, Mann–Whitney U test). The intensity of expression of this antigen increased in the HGSIL samples with respect to normal samples ( < 0.0068). sLex antigen could be a candidate to be used as biomarker for the early diagnosis of cervical cancer.

  9. Etiology and pathogenesis of precancerous lesions and invasive cervical carcinoma

    Directory of Open Access Journals (Sweden)

    Panjković Milana

    2008-01-01

    Full Text Available Cervical cancer is the second most common gynecological malignancy in the world. Human papilloma virus (HPV infection is the leading ethiologic agent in the development of premalignant and malignant cervical diseases. HPV is a member of the Papovaviridae family and until now over 100 types have been recognized. There are two types of viral infection: latent and productive. Virus induced oncogenesis is the result of interaction between virus oncoproteins E6 and E7 and tumor supresor host genes p53 and Rb. Many cofactors such as immunosuppression, early sexual relationship, multiple sexual partners, other sexualy transsmited infections and smooking are contributing factors of the precancerous and invasive cervical lesions. According to the oncogenic potential HPV are divided into three groups: low, intermediate and high oncogenic risk viruses. Molecular technics which are used for the virus detection are: In situ hibridisation,, Hybrid capture test and polymerasa chain reaction. Human papilloma virus testing has an important role in the follow up and treatment of women with 'atypical squamous cells of unknown significant' changes in cervical smears and low-grade squamous intraepithelial lesions, changes in punch biopsy.

  10. ASSESSING PROGRESSION OF CERVICAL PRE-CANCER LESIONS

    OpenAIRE

    Dragos Crauciuc; Ovidiu Toma; Eduard Crauciuc

    2016-01-01

    The purpose of this study was to accomplish a comparative assessment between the immune histochemical and the immunocytochemical expression of p16 protein and L1 major capsid protein of HPV respectively, in cervical squamous intraepithelial lesions with low and high grade, in order to determine, through morphological and clinical correlations, their applicability into practice when diagnosing and further monitoring the patients. There were 119 patients included in the study, havin...

  11. Co-expression of metalloproteinases 11 and 12 in cervical scrapes cells from cervical precursor lesions.

    Science.gov (United States)

    Valdivia, Alejandra; Peralta, Raúl; Matute-González, Manuel; García Cebada, Juan Manuel; Casasola, Ivonne; Jiménez-Medrano, Cristina; Aguado-Pérez, Rogelio; Villegas, Vanessa; González-Bonilla, Cesar; Manuel-Apolinar, Leticia; Ibáñez, Miguel; Salcedo, Mauricio

    2011-01-01

    The metalloproteinases (MMP) 11 and 12 have been shown to be expressed in cervical cancer (CC). In order to extend our previous results, these MMPs were evaluated in cervical precursor lesions. One hundred seventeen cervical scrapes: thirty-six normal, thirty-six low grade squamous lesions (LSIL), thirty-six high grade (HSIL), nine CC; and, also ninety-nine paraffin-embedded cervical lesions: fifteen normal cervices, thirty eight LSIL, sixteen HSIL, and five CC were collected. The samples were analyzed for relative expression by real time RT-PCR or immunohistochemistry assay. We were able to identify a relative increased expression of MMP11 in 75% and 78% from LSIL and HSIL samples, respectively. While MMP12 expression was 64% and 75% in LSIL and HSIL, respectively. Positive samples for MMP11 expression were also positive for MMP12 expression and also increased according to illness progression. In the tissues, MMP11 or MMP12 expression was observed in the cytoplasm of the neoplastic cells, while in the normal epithelium was absent. The reaction was always stronger for MMP12 than MMP11. MMP11 expression was present in 77% and 66% of LSIL and HSIL, while MMP12 expression was 73% and 68%. There was a relationship between MMP11 or MMP12 expression and HPV infection. Our data are showing a relationship between diagnostic of precursor lesions and the MMP11 and 12 expressions, suggesting that their expression could be an early event in the neoplastic lesions of the cervix and could have clinical significance.

  12. REPRODUCTIVE ASPECTS ASSOCIATED WITH PRECURSOR LESIONS FOR CERVICAL CANCER

    Directory of Open Access Journals (Sweden)

    Rogério Ferrari

    2013-05-01

    Full Text Available To investigate the relationship between reproductive variables and theprecursor lesions for cervical cancer in women attended the clinic of the lower genitaltract pathology and colposcopy (PTGIC, packed in the complex regional healthCaceres city, southwest of Mato Grosso, in the year 2009.Methods:We conducted across sectional study with data collection from medical records of 142 women withabnormal cytology, colposcopy and positive underwent directed biopsy, taking intoaccount the reproductive variables.Results:indicate that the minimum age at menarchewas 9 years and maximum age was 17 years, mean 12.6 years; on the average paritywas 3.4 children, the use of hormonal contraceptive method corresponds to 34 5% and38.1% female sterilization research, 46.5% are or have use of contraception for a periodgreater than five years, with a mean of 4.7 years and 67.2% of women surveyed do notuse condoms. The variables were not significantly associated to cervical cancer.Conclusion:the characteristics of the women studied may serve as a basis for workdirected to this population in order to seek to minimize this problem.Although the dataobtained were satisfactory, it was possible to trace the profile of the reproductiveaspects of women in the Clinic ofPTGIC, there is a lack of association betweenreproductive aspects and findings of the cervical biopsy, it may be associated with somelimiting factors of the study that need to be balanced

  13. Cervical Lesions in Women Younger than 25 Years

    Directory of Open Access Journals (Sweden)

    Yoleisi Alonso Fleites

    2011-12-01

    Full Text Available Fundamento: el cáncer cervical es esencialmente una enfermedad de transmisión sexual relacionada con la infección por el virus del papiloma humano. Este fenómeno ha aumentado globalmente, en especial en mujeres cada vez más jóvenes. Objetivo: determinar las lesiones de cuello uterino en mujeres menores de 25 años. Métodos: estudio observacional, descriptivo y correlacional que incluyó 248 pacientes registradas en el Servicio de Anatomía Patológica del Hospital General Universitario Dr. Gustavo Aldereguía Lima de Cienfuegos, en el periodo de enero a diciembre de 2007 y primer trimestre del 2008. Se analizaron: edad, edad de primera relación sexual, número de parejas sexuales, tipo de lesión, grado de la lesión y motivo de consulta. Resultados: predominaron las mujeres de más de 21 años y las que tuvieron 2 y 3 parejas sexuales; el principal motivo de consulta fue la cervicitis (84, 7 %; en el 22,6 % de las mujeres se detectó neoplasia intraepitelial grado I, en el 41, 9 % de grado II y en el 33, 1 % de grado III. En el 90 % de las pacientes más jóvenes la lesión fue  maligna. Conclusiones: la presencia de lesiones de cuello uterino en mujeres menores de 25 años no es baja, como tampoco la frecuencia de lesiones malignas.

  14. Stress Analysis of Occlusal Forces in Canine Teeth and Their Role in the Development of Non-Carious Cervical Lesions: Abfraction

    Directory of Open Access Journals (Sweden)

    Shihab A. Romeed

    2012-01-01

    Full Text Available Non-carious cervical tooth lesions for many decades were attributed to the effects of abrasion and erosion mainly through toothbrush trauma, abrasive toothpaste, and erosive acids. However, though the above may be involved, more recently a biomechanical theory for the formation of these lesions has arisen, and the term abfraction was coined. The aim of this study was to investigate the biomechanics of abfraction lesions in upper canine teeth under axial and lateral loading conditions using a three-dimensional finite element analysis. An extracted human upper canine tooth was scanned by μCT machine (Skyscan, Belgium. These μCT scans were segmented, reconstructed, and meshed using ScanIP (Simpleware, Exeter, UK to create a three-dimensional finite element model. A 100 N load was applied axially at the incisal edge and laterally at 45° midpalatally to the long axis of the canine tooth. Separately, 200 N axial and non-axial loads were applied simultaneously to the tooth. It was found that stresses were concentrated at the CEJ in all scenarios. Lateral loading produced maximum stresses greater than axial loading, and pulp tissues, however, experienced minimum levels of stresses. This study has contributed towards the understanding of the aetiology of non-carious cervical lesions which is a key in their clinical management.

  15. Prevalence and risk factors for cervical cancer and pre-cancerous lesions in Rwanda

    OpenAIRE

    Makuza, Jean Damascène; Nsanzimana, Sabin; Muhimpundu, Marie Aimee; Pace, Lydia Eleanor; Ntaganira, Joseph; Riedel, David James

    2015-01-01

    Introduction Cervical cancer prevalence in Rwanda has not been well-described. Visual inspection with acetic acid or Lugol solution has been shown to be effective for cervical cancer screening in low resource settings. The aim of the study is to understand the prevalence and risk factors for cervical cancer and pre- cancerous lesions among Rwandan women between 30 and 50 old undergoing screening. Methods This cross-sectional analytical study was done in 3 districts of Rwanda from October 2010...

  16. A Correlative Analysis of Cervical Lesions in Patients with Vulva Condyloma Acuminatum

    Institute of Scientific and Technical Information of China (English)

    Qing Li; Weihong Li; Huanzi Li; Zhihua Liu

    2006-01-01

    OBJECTIVE To analyze the relation ship among vulva condyloma acuminatum, high-risk human papillomavirus (HPV) infections and cervical epithelium lesions.METHODS From May 2002 to April 2004 patients with vulva condyloma acuminatum were examined employing vulva biospy, colposcopy, highrisk HPV-DNA test and cervical bioscopy.RESUTS In 418 cases of vulva condyloma acuminatum, verified by pathologic analysis, high-risk HPV (+) infections were detected in 68.7% (287/418) of the cases. Among those patients, 20.6% (59/287) had concurrent subclinical cervical intraepithelial neoplastic (CIN) lesions. Among the high-risk HPV(-) 31.3 % (131), patients 7.6% (10/131) had concurrent subclinical CIN lesions. Pathological examination results: cervicitis,167 (40.0%); cervical HPV infection, 182 (43.5%); CIN-Ⅰ, 51(12.2%); CIN-Ⅱ, 16 (3.83%); CIN-Ⅲ, 2 (0.5%); cervical cancer, none. Another patient had vulva condyloma acuminatum with valva intraepithelial neoplasia (VIN) Ⅱ~Ⅲ.CONCLUSION It was concluded that simultaneous cervical HPV infection of many types was rather common in patients with vulva condyloma acuminatum. Vulva condyloma acuminatum is the chief clinical symptom which hints at a high possibility of infection with high-risk HPV. The patients are at high-risk for CIN and cervical cancer. We must pay more attention to the cervix in cases with vulva condyloma acuminatum.

  17. Glass ionomer cements and their role in the restoration of non-carious cervical lesions

    Directory of Open Access Journals (Sweden)

    Luciana Fávaro Francisconi

    2009-10-01

    Full Text Available Glass ionomer based materials are clinically popular in several areas of restorative dentistry, but restoration of cervical lesions has proven particularly successful. Various etiologies, conformations, locations and structural characteristics make non-carious cervical lesions more challenging to adhesive restorative procedures and marginal seal in the long run. Due to their characteristics, glass ionomer cements (GICs have precise indication for these cases. Moreover, the use of a GIC base underneath composite resin, the so-called "sandwich" or mixed technique, allows associating the good characteristics of composite resins and GICs, and has been considered quite useful in the restoration of non-carious cervical defects. The aim of this paper is to critically review the literature and discuss peculiar features of GICs regarding their role in the restoration of non-carious cervical lesions.

  18. Distribution of HPV genotypes in cervical intraepithelial lesions and cervical cancer in Tanzanian women

    Directory of Open Access Journals (Sweden)

    Vidal Adriana C

    2011-11-01

    Full Text Available Abstract Background Infection with human papillomavirus (HPV is associated with uterine cervical intraepithelial neoplasia (CIN and invasive cancers (ICC. Approximately 80% of ICC cases are diagnosed in under-developed countries. Vaccine development relies on knowledge of HPV genotypes characteristic of LSIL, HSIL and cancer; however, these genotypes remain poorly characterized in many African countries. To contribute to the characterization of HPV genotypes in Northeastern Tanzania, we recruited 215 women from the Reproductive Health Clinic at Kilimanjaro Christian Medical Centre. Cervical scrapes and biopsies were obtained for cytology and HPV DNA detection. Results 79 out of 215 (36.7% enrolled participants tested positive for HPV DNA, with a large proportion being multiple infections (74%. The prevalence of HPV infection increased with lesion grade (14% in controls, 67% in CIN1 cases and 88% in CIN2-3. Among ICC cases, 89% had detectable HPV. Overall, 31 HPV genotypes were detected; the three most common HPV genotypes among ICC were HPV16, 35 and 45. In addition to these genotypes, co-infection with HPV18, 31, 33, 52, 58, 68 and 82 was found in 91% of ICC. Among women with CIN2-3, HPV53, 58 and 84/83 were the most common. HPV35, 45, 53/58/59 were the most common among CIN1 cases. Conclusions In women with no evidence of cytological abnormalities, the most prevalent genotypes were HPV58 with HPV16, 35, 52, 66 and 73 occurring equally. Although numerical constraints limit inference, findings that 91% of ICC harbor only a small number of HPV genotypes suggests that prevention efforts including vaccine development or adjuvant screening should focus on these genotypes.

  19. APPLICATION OF THE 2014 BETHESDA SYSTEM FOR REPORTING OF CERVICAL/VAGINAL CYTOLOGICAL LESIONS

    Directory of Open Access Journals (Sweden)

    Pratima

    2015-12-01

    Full Text Available Pap smear (Conventional smear is the most widely used cervical cancer screening test in the world. Negative intraepithelial lesion for malignancy, squamous intraepithelial lesion and cervical cancer remain important health problems for women worldwide. In developing countries like India, there is a great need for mass screening program for cervical lesion. AIMS AND OBJECTIVES To assess the clinician utility of The 2014 Bethesda System Reporting of cervical and vaginal epithelial abnormalities. MATERIAL AND METHODS All the cervical/vaginal cytology smears reported were retrieved and reviewed in the duration from 2007, 2008, 2009 and 2014. RESULTS A total number of 1700 cases of Pap smears were retrieved during period of study, out of which 90.0% were satisfactory for evaluation; 35.5% were within normal limit and 55.6% were NILM. A few (8.14% of the cases were reported as squamous intraepithelial lesion; 0.5% as atypical squamous cells and 0.19% were reported as atypical glandular cells. CONCLUSION Pap smear is a proven tested tool for making an early diagnosis and treating cervical cancer in early stage. Thus, Pap smear is simple, less expensive diagnostics tool suitable for implementation in India.

  20. Computed tomography in the foramen magnum and high cervical cord lesion

    Energy Technology Data Exchange (ETDEWEB)

    Isu, T.; Ito, T.; Iwasaki, Y.; Tsuru, M. (Hokkaido Univ., Sapporo (Japan). School of Medicine); Kitaoka, K.

    1981-10-01

    The value of computed tomography in the spine and spinal cord disease recently has been well documented. However, little attention is made to the role of computed tomography in foramen magnum and high cervical cord lesion. We report 16 cases of the foramen magnum and high cervical cord lesion. Method: Either EMI Head Scanner, CT 1010 (slice thickness 10 mm) or EMI Whole Body Scanner, CT 5005 (slice thickness 13 mm) was used. In 9 cases enhanced CT with intravenous injection of contrast medium was performed. Eleven cases received intrathecal injection of metrizamide. Materials: 1) control group without cervical pathology 70 cases, 2) spinal cord tumor 6 cases, 3) atlanto-axial dislocation 6 cases, 4) Chiari malformation (type 1) 3 cases, 5) spinal foreign body (acupuncture needle) 1 case. Results: 1. plain CT. 1) In control group without cervical pathology the cervical cord at C/sub 1/ level was seen as area surrounded by ring of subarachnoid space. 2) In 14 cases except for 1 case of atlanto-axial dislocation and 1 case of spinal foreign body identification of the cervical cord at C/sub 1/ level was impossible. 3) CT was of diagnostic value in detecting altanto-axial dislocation and spinal foreign body. 2. enhanded CT. Meningioma and neurinoma showed positive contrast enhancement. However, astrocytoma and herniated cerebellar tonsils were not detectable. 3. CT metrizamide myelography visualized the subarachnoid space and made it possible to localize the lesion in the spinal canal in relation to the spinal cord. Conclusion: Attention should be given to plain CT finding at C/sub 1/ level in diagnosis of foramen magnum and high cervical cord lesion. If identification of cervical cord at C/sub 1/ level is impossible, it is suspected that subarachnoid space is blocked by the lesion, and enhanced CT and CT metrizamide myelography must be performed.

  1. Correlation analysis of high-risk human papillomavirus viral load and cervical lesions

    Directory of Open Access Journals (Sweden)

    Xiao-xing MA

    2012-05-01

    Full Text Available Objective  To explore the association between high-risk human papillomavirus (HR-HPV viral load and pathological grades of cervical intraepithelial neoplasia (CIN and cervical cancer. Methods  A total of 1248 patients from General Hospital of PLA, who underwent colposcopy and surgery due to cervical lesions between Jan. 2006 and Aug. 2011 were enrolled in this study, and they were divided five groups: cervicitis, CIN Ⅰ, CIN Ⅱ-Ⅲ, stage Ⅰ cervical cancer and stage Ⅱ cervical cancer. HR-HPV viral load (RLU/CO was determined by the Hybrid Capture Ⅱ (HCⅡ system, and they were categorized into five groups: 0-0.99, 1.00-9.99, 10.00-99.99, 100.00-999.99, ≥1000.00. The mean value and standard deviation of different HR-HPV viral load in the patients with cervicitis or with CIN Ⅰ, CINⅡ-Ⅲ, stage Ⅰ cervical cancer or stage Ⅱ cervical cancer were compared, and the correlation of HR-HPV viral load and pathogenesis of cervical lesions was analyzed. Results  HPV viral loads were significantly higher in CINⅠ(842.1±983.9, CINⅡ-Ⅲ (690.1±795.0, stage Ⅰ cervical cancer (893.1±974.2 and stage Ⅱ cervical cancer (699.5±908.3 patients than in cervicitis patients (274.2±613.6, P < 0.05, and the HPV viral loads in CINⅠ(842.1±983.9 and stage Ⅰ cervical cancer patients were higher than those in CINⅡ-Ⅲ patients (P < 0.05. When HR-HPV viral load was ≥100RLU/CO, the risk of CIN and cervical cancer increased with the increase in viral load, but there was no correlation between the viral load and pathological grades of cervical lesions. In the patients with stage ⅠB-Ⅱ cervical squamous cell carcinoma, when the HR-HPV viral load was ≥100RLU/CO, the risk of lymph node metastasis increased (P < 0.05, and the number of patients with maximum diameter of the cervical tumor ≥4cm also increased (P < 0.05. However, the HR-HPV viral load was not correlated with patient age, pathological type of the lesion, depth of cancer

  2. Cervical intraepithelial lesions in females attending Women's Health Clinics in Alexandria, Egypt

    OpenAIRE

    Mona Abdel-Hadi; Adel Khalaf; Hanaa Aboulkassem; Noha Naeem; Mohamed Abdel Baqy; Hassan Sallam

    2015-01-01

    Background: Data from Egyptian studies provide widely varying estimates on the prevalence of preinvasive cervical lesions. The aim of this study was to estimate the rate of cervical intraepithelial neoplasia (CIN)   in Egyptian women living in Alexandria to clarify the need for implementing a national organized screening program and a vaccination program in our community. Materials and Methods: The study was conducted over a 6 years period and covered the different socioeconomic levels to hav...

  3. Location and Density of Immune Cells in Precursor Lesions and Cervical Cancer.

    Science.gov (United States)

    Bedoya, Astrid M; Jaramillo, Roberto; Baena, Armando; Castaño, Jorge; Olaya, Natalia; Zea, Arnold H; Herrero, Rolando; Sanchez, Gloria I

    2013-04-01

    Only a small proportion of women infected with Human Papillomavirus (HPV) develop cervical cancer. Host immune response seems to play a role eliminating the viral infection and preventing progression to cancer. Characterization of tumor infiltrating lymphocytes (TILs) in cervical pre-neoplastic lesions and cervical cancer may be helpful to understand the mechanisms that mediate this protection. The aim of this study was to determine if there are differences in the localization and density (cells/mm(2)) of CD8+ T-cells, CD4+ T-cells and Tregs (CD25 + Foxp3+) in cervical pre-neoplastic lesions and cervical cancer. Immunohistochemical analysis of sections of 96 (26 CIN1, 21 CIN2, 25 CIN3, and 24 SCC) samples revealed that regardless of CIN grades, CD8+ T-cells are more abundant than CD4+, CD25+ and Foxp3+ cells in both the stroma and epithelium. There was a higher density of CD8+ cells in the stroma of cervical cancer compared to CIN3 (OR = 4.20, 95% CI 1.2-15), CIN2 (OR = 7.86, 95% CI 1.7-36.4) and CIN1 (OR = 4.25, 95% CI 1.1-17). Studies evaluating whether these cells are recruited before or after cancer progression will be helpful to understand the role of these cells in the natural history of HPV-induced lesions.

  4. Human papillomavirus infection in couples with female low-grade intraepithelial cervical lesion.

    Science.gov (United States)

    Simon, Philippe; Roumeguere, Thierry; Christophe Noël, Jean

    2010-11-01

    Low-grade squamous intraepithelial lesions (LSIL) are frequently found during cervical cancer screening. Usually they are associated with a human papillomavirus (HPV) infection. Does the high-transmission rate of HPV infection to the male partner represent a clinical risk for him? Are preventive measures to be taken to prevent the occurrence of male diseases? More than 80% of all LSIL are associated with HPV infections. The prevalence of HPV infection in males can range up to 40%, with 60% of the male partners of LSIL female patients presenting with penile flat lesions. The spontaneous cure rate for male infections is very high (90% at 5 years) but negative consequences in females (cervical high-grade lesion and cervical cancer) are frequent. Their male counterparts are far rarer but in some patients can require deleterious treatment. Transmission prevention by the use of condoms and circumcision is discussed. The effectiveness of HPV vaccination in this situation has not been validated. PMID:20646823

  5. Dinamics of HPV Infection among Women with Cervical Lesions

    OpenAIRE

    E. Nikitina; Urazova, L.; Churuksaeva, O.

    2011-01-01

    A total of 293 women treated at Tomsk Cancer Research Institute were examined. HPV type 16 had the highest incidence rate (45.0%) followed by HPV 31-17,0%, HPV 56/33-15,0%, HPV 51/18/52-13,0%, HPV 58/35/39/45-7,0%, HPV 59-5,0%. Persistent infection was detected in 35.7% of primarily HPV-positive cases (10 out of 28 patients), mainly in cervical cancer patients. Total number of primarily HPV-positive and HPV-negative patients with cervical cancer was 95.0% and 5.0%, respectively. The correspon...

  6. Immunohistochemical expression of p16ink4a in inflammatory, preneoplastic and neoplastic cervical lesions

    Directory of Open Access Journals (Sweden)

    Gajanin Radoslav

    2015-01-01

    Full Text Available Introduction. High-risk human papilloma viruses play a main role in the development of cervical dysplasias and carcinomas. p16INK4a can be considered as a surrogate marker of active highrisk human papillomaviruses infection in dysplastic and neoplastic cells of the cervix. This study was aimed at determining the presence and level of p16INK4a expression in inflammatory, preneoplastic and neoplastic lesions of the cervix. Material and Methods. The study was performed on 109 samples of cervical biopsy. Cervical cancer was diagnosed in 36 patients, 34 patients had a preneoplastic change (dysplasia in stratified squamous cervix epithelium and a nonspecific inflammatory process was found in 39 patients. In all samples, immunohistochemical analysis using antibodies to p16INK4a was performed. Results. The expression of p16INK4a was verified in all cases of cervical cancer (100%, in 67.65% of dysplastic cervical lesions and in 38.5% of inflammatory lesions. A statistically highly significant difference was found in the presence and level of expression among neoplasic, dysplastic and inflammatory lesions of the cervix (χ² = 76.02, p < 0.001. The expression was more frequent and had a higher level in neoplastic and high grade dysplastic lesions compared to expression in inflammatory lesions and low grade dysplasias. Conclusion. The analysis of the presence of p16INK4a can differentiate non-neoplastic, high grade preneoplastic and neoplastic changes of the cervix. The use of p16INK4a in interpreting borderline lesions of the cervix can enable a rational therapeutic treatment of patients.

  7. Quantitative Detection of Screening for Cervical Lesions with ThinPrep Cytology Test

    Institute of Scientific and Technical Information of China (English)

    Hong-xin ZHANG; Yi-min SONG; Su-hong LI; Yu-hui YIN; Dong-ling GAO; Kui-sheng CHEN

    2010-01-01

    OBJECTIVE To investigate the available parameters in gynecological screening for cervical lesions by liquid-based cytology technology (ThinPrep Cytology Test, TCT) and The Bethesda System (TBS), also with computer image analysis. METHODS With application of the image analysis system, all grades of cervical lesion cells were detected quantitatively and sorted in atypical squamous cells of undetermined significance (ASCUS), atypical squamous cells-cannot exclude HSIL (ASC-H), low-grade squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL) and cervical squamous cell carcinoma (SCC) with the mean optical density (MOD), average grey (AG), positive units (PU), and nucleus to cytoplasmic ratio (N:C). Differences between each group of cells were compared and analyzed statistically.RESULTS Apart from four stereologic parameters in LSIL and HSIL groups there were no differences among them, in the other groups, there was statistically significant in differences between MOD, AG and PU values. Differences between them in the ratio of nucleus to cytoplasm were highly statistically signifi cant. CONCLUSION Stereological indexes may serve as a screening tool for cervical lesions. The image analysis system is expected to become a new means of cytological assisted diagnosis.

  8. 5-year clinical performance of resin composite versus resin modified glass ionomer restorative system in non-carious cervical lesions

    DEFF Research Database (Denmark)

    Franco, Eduardo Batista; Benetti, Ana Raquel; Ishikiriama, Sérgio Kiyoshi;

    2006-01-01

    To comparatively assess the 5-year clinical performance of a 1-bottle adhesive and resin composite system with a resin-modified glass ionomer restorative in non-carious cervical lesions.......To comparatively assess the 5-year clinical performance of a 1-bottle adhesive and resin composite system with a resin-modified glass ionomer restorative in non-carious cervical lesions....

  9. Air Abrasion

    Science.gov (United States)

    ... delivered directly to your desktop! more... What Is Air Abrasion? Article Chapters What Is Air Abrasion? What Happens? The Pros and Cons Will I Feel Anything? Is Air Abrasion for Everyone? print full article print this ...

  10. Performance Evolution of Various Wavelets in Cervical Lesion Detection

    Directory of Open Access Journals (Sweden)

    P S RAMAPRABA

    2014-01-01

    Full Text Available Cervical cancer is one of most common cancers among women in the world caused by human papilloma virus infection. It develops in the tissue of cervix which connects upper body of the uterus to the vagina. The types of cancer are squamous cell carcinoma, adeno carcinoma and adeno squamous carcinoma based on location of cervix where cancer develops. In this paper, an automatic detection of squamous cell carcinoma in cervical images based on Discrete Wavelet Transform (DWT and K-Nearest Neighbor (KNN classifier is described. The energy features are extracted from DWT decomposed image of small area of cervical images. Then the features are fed into KNN classifier to classify whether the given area is normal or cancer affected region. The performance of the proposed system is evaluated by using three wavelets namely bi-orthogonal (bior3.7, Daubechies-8(db8 and Symlet (sym8. Experimental results show the performance of db8 with other wavelets that produces 97.22% average accuracy.

  11. A study of metastatic lesions of cervical lymphadenopathy by fine needle aspiration cytology

    Directory of Open Access Journals (Sweden)

    Deepa Jethwani

    2015-07-01

    Conclusion: FNAC is a cost effective, reliable, rapid and inexpensive method for diagnosis of lymphadenopathy. Cytology of Metastatic lesions in cervical lymph nodes gives clue to nature and origin of tumors. [Int J Res Med Sci 2015; 3(7.000: 1697-1700

  12. Prevalence of human papillomavirus DNA in female cervical lesions from Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    S. M. B. Cavalcanti

    1994-12-01

    Full Text Available A hundred-sixty paraffin-embedded specimens from female cervical lesions were examined for human papillomavirus (HPV types 6, 11, 16 and 18 infections by non-isotopic in situ hybridization. The data were compared with histologic diagnosis. Eighty-eight (55 biopsies contained HPV DNA sequences. In low grade cervical intraepithelial neoplasias (CIN I, HPV infection was detected in 78.7 of the cases, the benign HPV 6 was the most prevalent type. HPV DNA was detected in 58 of CIN II and CIN III cases and in 41.8 of squamous cell carcinomas (SCC. Histologically normal women presented 20 of HPV infection. Oncogenic HPV was found in 10 of these cases, what may indicate a higher risk of developing CINs and cancer. Twenty-five percent of the infected tissues contained mixed infections. HPV 16 was the most common type infecting the cervix and its prevalence raised significantly with the severity of the lesions, pointing its role in cancer pathogenesis. White women presented twice the cervical lesions of mulatto and African origin women, although HPV infection rates were nearly the same for the three groups (approximately 50. Our results showed that HPV typing by in situ hybridization is a useful tool for distinguishing between low and high risk cervical lesions. Further studies are required to elucidate risk factors associated with HPV infection and progression to malignancy in Brazilian population.

  13. Relationship between hTERC gene and precancerous lesion as well as occurrence and development of cervical cancer

    Institute of Scientific and Technical Information of China (English)

    Li-Hong Yi

    2016-01-01

    Objective:To study the relationship betweenhTERC gene and precancerous lesion as well as occurrence and development of cervical cancer.Methods:A total of 120 cases receiving cervical biopsy in our hospital from May 2012 to December 2014 were selected for study, 45 cases of normal cervical tissue and inflammatory cervical tissue, 37 cases of cervical intraepithelial neoplasia tissue and 38 cases of cervical cancer tissue were included.hTERC gene expression, oncogene expression and invasive molecule contents in cervical tissue were detected.Results:Green signal copy number and red signal copy number ofhTERC in cervical cancer tissue were significantly higher than those in cervical intraepithelial neoplasia tissue and normal cervical tissue, and the contents of hTERTand hTP were not different from those in cervical intraepithelial neoplasia tissue and normal cervical tissue; the contents of proteins encoded by p63, TNFAIP8, SRX, P459arom and Piwil2 as well as the contents of RbAp48, Furin, MT1-MMP, TGF-β1, FoxM1, uPA and tPA in cervical cancer tissue were significantly higher than those in cervical intraepithelial neoplasia tissue and normal cervical tissue; the contents of proteins encoded by p63, TNFAIP8, SRX, P459arom and Piwil2 as well as the contents of RbAp48, Furin, MT1-MMP, TGF-β1, FoxM1, uPA and tPA in cervical cancer tissue with high expression of hTERC were significantly higher than those in cervical cancer tissue with low expression of hTERC.Conclusions:Activation of telomerase and increase of hTERC expression are involved in the precancerous lesion as well as the occurrence and development of cervical cancer, and hTERC can activate the expression of oncogenes and.

  14. One-year clinical evaluation of tooth-coloured materials in non-carious cervical lesions

    Directory of Open Access Journals (Sweden)

    Stojanac Igor

    2011-01-01

    Full Text Available Introduction. The restoration of non-carious cervical lesions is specific, mostly because of the location of their margins, especially the cervical margin, which is determined in cement and/or dentine. This feature makes the cervical margin more susceptible to micro-leak­age, causing marginal discoloration, postoperative sensitivity, development of the secondary caries and loss of the restoration. Material and methods. One of the criteria for inclusion of a patient in this study was the presence of at least two non-carious cervical lesions to be restored with the minimum depth of 1mm, independently of their location in the dental arch. A total of 60 restorations were placed in 30 patients, and every patient received both tested materials (composite resins and compomer on their non-carious cervical lesions. The clinical evaluation of the therapeutic success was performed six months and then one year after the day of the placement of restorations using the modified-United States Public Health Service criteria. The following was evaluated: retention, marginal integrity, marginal discoloration, wear, postoperative sensitivity and secondary caries. Results. A statistically significant high percent of restorations/teeth with postoperative sensitivity was found in the group of resin composite restorations after six months. At the end of the evaluation period, that is after one year, there were no statistically significant differences between materials for all evaluated criteria. Conclusion. The results of this study show the identical quality of both examined materials one year after the readjustment of non-carious cervical lesions.

  15. Percutaneous anterolateral balloon kyphoplasty for metastatic lytic lesions of the cervical spine.

    Science.gov (United States)

    Lykomitros, Vasilis; Anagnostidis, Kleovoulos S; Alzeer, Ziad; Kapetanos, George A

    2010-11-01

    The purpose of our report is to describe a new application of kyphoplasty, the percutaneous anterolateral balloon kyphoplasty that we performed in two cases of metastatic osteolytic lesions in cervical spine. The first patient, aged 48 years, with primary malignancy in lungs had two metastatic lesions in C2 and C6 vertebrae. Patient's complaints were about pain and restriction of movements (due to the pain) in the cervical spine. The second patient, aged 70 years, with primary malignancy in stomach, had multiple metastatic lesions in thoracolumbar spine and C3, C4 and C5 vertebrae without neurological symptoms. The main symptoms were from cervical spine with severe pain even in bed rest and systematic use of opiate-base analgesic. The preoperative status was evaluated with X-rays, CT scan, MRI scan and with Karnofsky score and visual analogue pain (VAS) scale. Both patients underwent percutaneous anterolateral balloon kyphoplasty via the anterolateral approach in cervical spine under general anaesthesia. No clinical complications occurred during or after the procedure. Both patients experienced pain relief immediately after balloon kyphoplasty and during the following days. The stiffness also resolved rapidly and cervical collars were removed. VAS score significantly improved from 85 and 95 preoperatively to 30 in both patients. Karnofsky score showed also improvement from 40 and 30 preoperatively to 80 and 70, respectively, at the final follow-up (7 months after the procedure). Fluoroscopy-guided percutaneous anterolateral balloon kyphoplasty proved to be safe and effective minimally invasive procedure for metastatic osteolytic lesions of the cervical spine, reducing pain and avoiding vertebral collapse. Experience and attention are necessary in order to avoid complications.

  16. Comparison of MRI pulse sequences for investigation of lesions of the cervical spinal cord

    Energy Technology Data Exchange (ETDEWEB)

    Campi, A.; Pontesilli, S.; Gerevini, S.; Scotti, G. [San Raffaele Hospital, Milan (Italy). Dept. of Neuroradiology

    2000-09-01

    Small spinal cord lesions, even if clinically significant, can be due to the low sensitivity of some pulse sequences. We compared T2-weighted fast (FSE), and conventional (CSE) spin-echo and short-tau inversion-recovery (STIR)-FSE overlooked on MRI sequences to evaluate their sensitivity to and specificity for lesions of different types. We compared the three sequences in MRI of 57 patients with cervical spinal symptoms. The image sets were assessed by two of us individually for final diagnosis, lesion detectability and image quality. Both readers arrived at the same final diagnoses with all sequences, differentiating four groups of patients. Group 1 (30 patients, 53 %), with a final diagnosis of multiple sclerosis (MS). Demyelinating lesions were better seen on STIR-FSE images, on which the number of lesions was significantly higher than on FSE, while the FSE and CSE images showed approximately equal numbers of lesions; additional lesions were found in 9 patients. The contrast-to-noise ratio (CNR) of 17 demyelinating lesions was significantly higher on STIR-FSE images than with the other sequences. Group 2, 19 patients (33 %) with cervical pain, 15 of whom had disc protrusion or herniation: herniated discs were equally well delineated with all sequences, with better myelographic effect on FSE. In five patients with intrinsic spinal cord abnormalities, the conspicuity and demarcation of the lesions were similar with STIR-FSE and FSE. Group 3, 4 patients (7 %) with acute myelopathy of unknown aetiology. In two patients, STIR-FSE gave better demarcation of lesions and in one a questionable additional lesions. Group 4, 4 patients (7 %) with miscellaneous final diagnoses. STIR-FSE had high sensitivity to demyelinating lesions, can be considered quite specific and should be included in spinal MRI for assessment of suspected demyelinating disease. (orig.)

  17. Extracranial Vertebral Artery Aneurysm Presenting as a Chronic Cervical Mass Lesion

    Directory of Open Access Journals (Sweden)

    Lampis C. Stavrinou

    2010-01-01

    Full Text Available Background. Aneurysms of the extracranial vertebral artery are rare and can provide a diagnostic and therapeutic challenge. Methods. We reviewed the clinical history of a patient presenting with cervical radiculopathy, who harboured an extracranial vertebral artery aneurysm eroding the cervical spine. Results. CT Angiography and MR Angiography set the diagnosis, by revealing a left C5-C6 vertebral artery aneurysm with cervical root impingement. Bony reconstruction depicted enlargement of the C6 transverse foramen and a marked enlargement of the C6-C7 intravertebral foramen. The lesion was treated by intravascular proximal vertebral artery occlusion. Conclusions. Extracranial vertebral artery aneurysms require a high index of clinical suspicion. This is the first report of a vertebral artery pseudoaneurysm presenting with bony erosion, which supports a less minacious portrayal of vertebral artery aneurysms.

  18. The DNA load of six high-risk human papillomavirus types and its association with cervical lesions

    OpenAIRE

    Río Ospina, Luisa del; Soto de León, Sara; Camargo, Milena; Moreno Pérez, Darwin Andrés; Sánchez, Ricardo; Pérez Prados, Antonio; Patarroyo, Manuel Elkin; Patarroyo, Manuel Alfonso

    2015-01-01

    Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency. Methods: 114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them...

  19. Increased expression of sialic acid in cervical biopsies with squamous intraepithelial lesions

    Directory of Open Access Journals (Sweden)

    Vallejo-Ruiz Verónica

    2010-11-01

    Full Text Available Abstract Background Altered sialylation has been observed during oncogenic transformation. Sialylated oligosaccharides of glycoproteins and glycolipids have been implicated in tumor progression and metastases. In the cervical cancer high levels of sialic acid have been reported in the patients serum, and an increased of total sialic acid concentration has been reported for the cervical neoplasia and cervical cancer. This study investigates the changes in expression and distribution of α2,3-linked sialic acid and α2,6- linked sialic acid in low and high squamous intraepithelial lesions and in normal tissue. Methods Lectin histochemistry was used to examine the expression and distribution of sialic acid in different grades of cervical neoplasia. We applied Maackia amurensis lectin, which interacts with α2,3-linked sialic acid and Sambucus nigra lectin specific for α2,6-linked sialic acid. Results The histochemical analysis showed that α2,3-linked sialic acid and α2,6- linked sialic acid increased in intensity and distribution in concordance with the grade of squamous intraepithelial lesion (SIL. These results are in concordance with a previous study that reports increased RNAm levels of three sialyltransferases. Conclusions These results show that the change in sialylation occurs before cancer development and may play an important role in cellular transformation. These findings provide the basis for more detailed studies of the possible role of cell surface glycoconjugates bearing sialic acid in the cellular cervix transformation.

  20. Human Papillomavirus Types 52 and 58 Are Prevalent in Uterine Cervical Squamous Lesions from Japanese Women

    Directory of Open Access Journals (Sweden)

    Kazuhiro Takehara

    2011-01-01

    Full Text Available Objective. To estimate the prevalence and genotypes of high-risk human papillomavirus (HPV focusing HPV 16, 18, 52, and 58 in Japan. Methods. Liquid-base cytology specimens were collected from Japanese women (n=11022, aged 14–98. After classifying cytodiagnosis, specimens were analyzed for HPV DNA by the multiplex polymerase chain reaction method, where 1195 specimens were positive for cervical smear, except adenomatous lesions. Result. HPV genotypes were detected in 9.5% of NILM and 72.2% of ASC-US or more cervical lesions. In positive cervical smears, HPV genotypes were HPV 52 at 26.6%, HPV 16 at 25.2%, HPV 58 at 21.8%, and HPV 18 at 7.1%. Most patients infected with HPV 16 were between 20–29 years old, decreasing with age thereafter. As for HPV 52 and 58, although the detection rate was high in 30- to 39-year-olds, it also was significant in the 50s and 60s age groups. Conclusion. In Japan, as a cause of abnormal cervical cytology, HPV52 and 58 are detected frequently in addition to HPV 16. In older age groups, HPV 52 and 58 detection rates were higher than that observed for HPV 16. After widespread current HPV vaccination, we still must be aware of HPV 52 and 58 infections.

  1. Telomerase activity in high-grade cervical lesions is associated with allelic imbalance at 6Q14-22.

    NARCIS (Netherlands)

    Duin, van M.; Steenbergen, R.D.M.; Wilde, de J.; Helmerhorst, TJ; Verheijen, R.H.M.; Risse, E.K.J.; Meijer, C.J.L.M.; Snijders, P.J.F.

    2003-01-01

    Our study attempts to establish the relationship between telomerase activity and allelic imbalance (AI) on chromosomes 3p and 6 in high-risk HPV-containing cervical lesions. These chromosomes were implicated previously in telomerase regulation in HPV containing immortalized cells and cervical cancer

  2. Osteosíntesis cervical posterior con placa en lesiones cervicales

    OpenAIRE

    Sánchez Martín, Miguel María

    2002-01-01

    El propósito del presente estudio es analizar retrospectivamente los resultados a largo plazo de las lesiones de la columna cervical baja tratadas mediante estabilización posterior con placas atornilladas de Roy-Cmille a las masas laterales. Pacientes y métodos: Se estudian 15 pacientes, de los cuales sólo 14 completos con lesiones traumáticas (13) y 1 no traumática (1 paciente con inestabilidad sobre artrosis por doble Cloward) durante un periodo postoperatorio medio de 8 años (6-12). Salvo ...

  3. High grade squamous intraepithelial lesion in inmates from Ohio: cervical screening and biopsy follow-up

    Directory of Open Access Journals (Sweden)

    Rofagha Soraya

    2006-01-01

    Full Text Available Abstract Background Cervical carcinoma remains the second leading cause of cancer death in women worldwide and sexual behavior is regarded as the main contributing factor. We studied cervical cytology screening with surgical biopsy follow-up in women prisoners and compared the findings to those in the general population. Methods We reviewed 1024 conventional cervical smears, 73 cervical biopsies and 2 loop electrosurgical excision procedure (LEEP specimens referred to us from the Correctional Center in Columbus, Ohio during a 12-month period. The results were compared to 40,993 Pap smears from the general population for the same 12-month period. Results High grade squamous intraepithelial lesion (HGSIL was diagnosed in 1.3% of the cervical smears from the inmate population versus 0.6% in the general population (p < 0.01. The unsatisfactory rate was 1.6% compared to 0.3% in the general population (p < 0.01. Among the study population, follow-up tissue diagnosis was obtained in 24.3% of the abnormal cytology results (ASCUS, LGSIL, and HGSIL. Of the HGSIL Pap smears, 61.5% had a subsequent tissue diagnosis. Thirty-nine biopsies (52% of the all inmate biopsies and LEEP showed CIN II/III (cervical intraepithelial neoplasia II/III. Eight of these thirty-nine follow-up biopsies diagnosed as CIN II/III had a previous cervical cytology diagnosis of ASCUS. The average age for HGSIL was 30.5 years (S.D. = 5.7 and for low grade squamous intraepithelial lesion (LGSIL was 27.2 years (S.D. = 6.1. Conclusion A significantly higher prevalence of HGSIL cervical cytology and unsatisfactory smears was encountered in female inmates, with tissue follow-up performed in less than two thirds of the patients with HGSIL. These results are in keeping with data available in the literature suggesting that the inmate population is high-risk and may be subject to less screening and tissue follow-up than the general population. Clinicians should proceed with urgency to improve

  4. Genotype distribution of cervical human papillomavirus DNA in women with cervical lesions in Bioko, Equatorial Guinea

    Directory of Open Access Journals (Sweden)

    Carro-Campos Patricia

    2009-09-01

    Full Text Available Abstract Background The HVP vaccine is a useful tool for preventing cervical cancer. The purpose of this study is to determine the most frequent HPV genotypes in Equatorial Guinea in order to develop future vaccination strategies to apply in this country. Methods A campaign against cervical cancer was carried out in the area on a total of 1,680 women. 26 of the women, following cytological screening, were treated surgically with a loop electrosurgical excision procedure (LEEP. Cases were studied histologically and were genotyped from paraffin blocks by applying a commercial kit that recognized 35 HPV types. Results Cytological diagnoses included 17 HSIL, 1 LSIL, 5 ASC-H and 3 AGUS. Histological diagnosis resulted in 3 cases of microinvasive squamous cell carcinoma stage IA of FIGO, 9 CIN-3, 8 CIN-2, 2 CIN-1, 3 flat condylomas and mild dysplasia of the endocervical epithelium. Fifteen of twenty-five cases genotyped were positive for HPV (60%. HPV 16 and 33 were identified in four cases each, HPV 58 in two other cases, and HPV 18, 31, 52, and 82 in one case, with one HPV 16 and 58 coinfection. Conclusion The frequency of HPV types in the African area varies in comparison to other regions, particularly in Europe and USA. Vaccination against the five most common HPV types (16, 33, 58, 18, and 31 should be considered in the geographic region of West Africa and specifically in Equatorial Guinea.

  5. Methylated Host Cell Gene Promoters and Human Papillomavirus Type 16 and 18 Predicting Cervical Lesions and Cancer.

    Directory of Open Access Journals (Sweden)

    Nina Milutin Gašperov

    Full Text Available Change in the host and/or human papillomavirus (HPV DNA methylation profile is probably one of the main factors responsible for the malignant progression of cervical lesions to cancer. To investigate those changes we studied 173 cervical samples with different grades of cervical lesion, from normal to cervical cancer. The methylation status of nine cellular gene promoters, CCNA1, CDH1, C13ORF18, DAPK1, HIC1, RARβ2, hTERT1, hTERT2 and TWIST1, was investigated by Methylation Specific Polymerase Chain Reaction (MSP. The methylation of HPV18 L1-gene was also investigated by MSP, while the methylated cytosines within four regions, L1, 5'LCR, enhancer, and promoter of the HPV16 genome covering 19 CpG sites were evaluated by bisulfite sequencing. Statistically significant methylation biomarkers distinguishing between cervical precursor lesions from normal cervix were primarily C13ORF18 and secondly CCNA1, and those distinguishing cervical cancer from normal or cervical precursor lesions were CCNA1, C13ORF18, hTERT1, hTERT2 and TWIST1. In addition, the methylation analysis of individual CpG sites of the HPV16 genome in different sample groups, notably the 7455 and 7694 sites, proved to be more important than the overall methylation frequency. The majority of HPV18 positive samples contained both methylated and unmethylated L1 gene, and samples with L1-gene methylated forms alone had better prognosis when correlated with the host cell gene promoters' methylation profiles. In conclusion, both cellular and viral methylation biomarkers should be used for monitoring cervical lesion progression to prevent invasive cervical cancer.

  6. One-year clinical evaluation of compomer restorations in cervical lesions of different aetiology

    Directory of Open Access Journals (Sweden)

    Premović Milica

    2012-01-01

    Full Text Available Introduction. The aim of this one-year prospective clinical study was to evaluate the treatment results of compomer restorations (Dyract® eXtra/ Dentsply/De Trey, Konstanz, Germany with a single step self-etching dental adhesive (Xeno® III Dentsply/De Trey, Konstanz, Germany used for restoring class V lesions (non-carious and primary carious cervical lesions. Material and Methods. A total number of 62 class V restorations (n = 62 were placed by one dentist in 30 patients on incisors, canines and premolars. The fillings were placed due to different indications: non-carious cervical defects (n = 32 and primary carious lesions (n = 30. The restorations were evaluated by a single-blind design, according to the Modified United States Public Health Service system 6 and 12 months following the placement. The following were evaluated: retention, marginal integrity, marginal discoloration, wear, postoperative sensitivity and secondary caries. The statistical analysis compared the ratings of each criteria between materials using the Pearson chi-square or Fisher’s exact test at a level of significance of 5% (p<0.05. Results. Two restorations of the non-carious lesion group were lost after 6 months, and after 12 months one restoration was lost in the group of primary carious lesions. There were no statistically significant differences between restorations for all evaluated criteria in both groups. Conclusion. The compomer restorations in combination with a single step self-etching dental adhesive showed acceptable clinical performance in Class V lesions after one year of clinical service.

  7. Prevalence of human papillomavirus types in cervical cancerous and precancerous lesions of Ecuadorian women.

    Science.gov (United States)

    Mejía, Lorena; Muñoz, Diana; Trueba, Gabriel; Tinoco, Leopoldo; Zapata, Sonia

    2016-01-01

    Human Papillomavirus (HPV) is the most common sexually transmitted infection worldwide and it is responsible for most cases of uterine cancer. In Ecuador there is limited information about HPV types (and variants) in cancerous lesions; however, identifying the type-specific HPV prevalence in cervical lesions of women living in Ecuador is important to better predict the impact of HPV prophylactic vaccination in this country. We studied the prevalence of HPV types in cervical cancerous or precancerous lesions from 164 Ecuadorian women and found that 86.0% were HPV positive. The most common types were HPV16 (41.8%) and HPV58 (30.5%). Interestingly, HPV18 was detected only in 2.8% of the HPV-positive samples. Fifteen DNA sequences (genes E6 and L1) from 16 samples positive for HPV16 belonged to the European lineage, considered one of the least carcinogenic lineages, and 1 (6.25%) to the Asian-American lineage. Similar analysis in 12 HPV58 positive samples showed that 10 (83.3%) sequences grouped in sublineage A2, which belongs to the oldest HPV58 lineage, 1 belonged to A3 and 1 to lineage C. This study suggests that the currently used HPV vaccines (bivalent and tetravalent) may have lower effectiveness in Ecuador than in other geographic locations where HPV18 is more prevalent. PMID:26113443

  8. Cervical intraepithelial lesions in females attending Women′s Health Clinics in Alexandria, Egypt

    Directory of Open Access Journals (Sweden)

    Mona Abdel-Hadi

    2015-01-01

    Full Text Available Background: Data from Egyptian studies provide widely varying estimates on the prevalence of preinvasive cervical lesions. The aim of this study was to estimate the rate of cervical intraepithelial neoplasia (CIN   in Egyptian women living in Alexandria to clarify the need for implementing a national organized screening program and a vaccination program in our community. Materials and Methods: The study was conducted over a 6 years period and covered the different socioeconomic levels to have a representative sample for women living in Alexandria. All women included did not have any cervical disorder related complaints. Conventional Pap smears were obtained and diagnosed using the Bethesda system. Women with abnormal Pap smears were managed according to the 2006 consensus guidelines within the available facilities. Persistent abnormal cytological results were referred for colposcopic biopsy. Histological results were grouped into: Reactive changes, CIN 1, CIN 2/CIN 3 and adenocarcinoma in-situ (AIS. Results: Out of the 6173 smears included in the study 6072 (98.36% were normal and only 101 (1.63% were abnormal. After colposcopic biopsies, 0.08% had CIN 1, 0.03% had CIN 2, 3 and 0.01% had AIS. Conclusion: We concluded that cervical cancer screening programs, although life-saving for a number of women, are not a sufficiently high priority in our community. Money for national health screening programs should preferably be directed more towards recruiting women for breast cancer screening, since breast cancer accounts for about 33% of all female cancers in Egypt ranking number one, while cervical cancer ranks number 13.

  9. The Prevalence of Human Papilloma Virus(HPV in Malignant Cervical Lesion, Using Multiplex PCR

    Directory of Open Access Journals (Sweden)

    M. R. Keyhkhaee

    2006-07-01

    Full Text Available Background: Cervical cancer is the second leading cause of cancer death among women. In this cancer, the effects of prevention, early diagnosis and treatment more than other cancers decrease the mortality rate. In 1970 human papilloma virus (HPV was introduction as major etiologic factor of cervical cancer. Different studies throughout the world revealed strong correlation between HPV and cancerous & precancerous changes in epithelial cells. Since cell culture and serological methods can not recognize the virus and its subtypes, the importance of the molecular methods including polymerase chain reaction (PCR in early and definite diagnosis of virus is obvious. Methods: In this study, after patient selection using the related protocol and completion of the questionnaires, 100 samples from cancer lesions of cervix selected. Then DNA extraction from paraffin blocks performed using standard method. Multiplex PCR with two pairs of primer (one as internal control performed and the PCR product run on 8% polyacrylamid gel. Results: The results showed that 73% of the tissues were infected by HPV. Conclusion: This finding confirm the previous results based of correlation between HPV,and cervical cancer.

  10. EPIDERMAL GROWTH FACTOR RECEPTOR (EGFR AND HUMAN PAPILLOMAVIRUS (HPV L1 CAPSID PROTEIN IN CERVICAL SQUAMOUS INTRAEPITHELIAL LESIONS

    Directory of Open Access Journals (Sweden)

    Balan Raluca

    2010-09-01

    Full Text Available We analyzed the immunohistochemical pattern of epidermal growth factor receptor (EGFR in cervical squamous intraepithelial lesions (SILs in correlation with L1 HPV capsid protein, in order to determine the relationship between EGFR expression and the infection status of human papillomavirus (HPV. The study included 40 cases, 24 LSIL (low grade SIL (CIN1, cervical intraepithelial neoplasia and 16 HSIL (high grade SIL (6 cases of CIN2 and 10 cases of CIN3. The immunoexpression of L1 HPV protein was assessed on conventional cervico-vaginal smears and EGFR was immunohistochemically evaluated on the corresponding cervical biopsies. The HPV L1 capsid protein was expressed in 45.83% of LSIL and 25% of HSIL. EGFR was overexpressed in 62,4% of HSIL (58,4% CIN2 and 41,6% CIN3 and 37,6% LSIL. The immunoexpression of L1 HPV has clinical application in the progression assessment of the cervical precancerous lesions without a correlation to the grade of the cervical SIL. EGFR is expressed by all proliferating squamous epithelial cells, thus corresponding with the grade of SIL. The evaluation of EGFR status, correlated with L1 HPV protein expression, can provide useful data of progression risk of cervical squamous intraepithelial lesions

  11. Diagnostic tests for the detection of human papillomavirus-associated cervical lesions.

    Science.gov (United States)

    Reuschenbach, Miriam; von Knebel Doeberitz, Magnus

    2013-01-01

    Current diagnostic approaches for primary cervical cancer screening, work-up of equivocal or positive screening results or follow- up after treatment of precancerous lesions primarily rely on the morphologic interpretation of squamous epithelial cells (Pap cytology), in some setting accompanied by the detection of human papillomavirus DNA and have largely contributed to remarkable reduction of disease incidence in countries with implemented screening programs. However, these approaches are limited by a poor sensitivity and reproducibility of Pap cytology and low specificity for high grade cervical intraepithelial neoplasia of HPV DNA detection assays. Early detection might be improved by complementing or even replacing these tests by markers which are more directly related to molecular events triggering HPV-induced carcinogenesis and thereby might deliver more accurate diagnostic performance. The delineation of molecular changes which occur during different stages of HPV infections and the identification of changes which induce neoplastic alterations allow for the detection of markers that specifically highlight the transforming stage of the infection where viral oncogenes are overexpressed and therefore allow for a more specific diagnosis of lesions that require treatment. The evaluation of such markers in clinical studies revealed that some indeed show an improved diagnostic performance compared to Pap cytology or HPV DNA tests only.

  12. Reliability of the CINtecTM p16INK4a immunocytochemical test in screening cervical precancerous lesions

    Directory of Open Access Journals (Sweden)

    Jović Milena

    2008-01-01

    Full Text Available Background/Aim. Overexpression of p16INK4a has been found to be linked with genomic integration of high-risk human papillomavirus (HPV and the developement of precancerous cervical intraepithelial lesions. The aim of this study was to examine is there a higher positive level of correlation between grade of histological dysplasia and p16INK4a level of expression in cervical smear, compared to results of Papanicolaou test. We also examined the correlation between HPV type, p16INK4a expression and Papanicolau test results. Methods. A total of 48 women with precanceorous cervical lesions and HPV cervicitis and 10 healthy women were enrolled in the study. Papanicolaou test, CINtecTM p16INK4a citological immunohistochemical test, polymerase chain reaction (PCR HPV 16, 18, 31, 33 analysis and histopathology of the lesion were performed in all the patients. Results. Comparing the results of Papanicoulaou test and the grade of histological dysplasia, low-grade squamous intraepithelial lesion (LSIL was confirmed in 38%, and high-grade squamous intraepithelial lesion (HSIL in 69.2% of the patients (p > 0.05. Significant positive correlation was found between p16 overexpression and grade of histological dysplasia (p = 0.000. Overexpression p16 was found in 70% of LSIL and 94.4% of HSIL. Positive correlation was found between p16 overexpression and grade of dysplasia in Papanicolaou test (p = 0.011. In 38% of LSIL and 15% of HSIL cases p16 was not expressed. The most frequently found HPV type in PCR analysis was HPV16. Analysing the results of p16 test according to HPV status and Papanicolaou test rather heterogenous results were obtained. Conclusion. In the patients with precancerous cervical lesions a higher level of correlation was found between the grade of histological dysplasia and p16INK4a level of expression in the cervical smear, compared to the results of Papanicolaou test.

  13. Human Papillomavirus Genotyping and p16INK4a Expression in Cervical Lesions: A Combined Test to Avoid Cervical Cancer Progression

    Science.gov (United States)

    Zouheir, Yassine; Fechtali, Taoufiq; Elgnaoui, Nadia

    2016-01-01

    Cervical cancer is a major public health problem in Morocco. The cervical cancer has a long precancerous period that provides an opportunity for the screening and treatment. Improving screening tests is a priority goal for the early diagnosis of cervical cancer. This study was conducted to evaluate the combination of p16INK4a protein expression, human papillomavirus (HPV) typing, and histopathology for the identification of cervical lesions with high risk to progress to cervical cancer among Moroccan women. A total of 96 cervical biopsies were included in this study. Signal amplification in situ hybridization with biotinylated probes was used to detect HPV. Immunohistochemistry was used to evaluate the expression of p16INK4a protein. HPV DNA was detected in 74.0% of the biopsies (71/96). Of the seventy-one positive HPV cases, we detected 67.6% (48/71) of high risk (HR)-HPV (HPV 16 and 18), 24% of low risk-HPV (HPV 6 and 11), 1.4% intermediate risk-HPV (HPV 31, 33, and 35), and 7% coinfections (HPV 6/11 and 16/18). Overexpression of p16INK4a protein was observed in 72.9% (70/96) of the biopsies. In addition, p16INK4a protein detection was closely correlated with recovery of HR HPV. Our result showed that p16INK4a expression level is correlated with HR-HPV status. PMID:27390742

  14. Utility of Papanicolaou test in diagnosis of cervical lesions: a study in a tertiary care centre of western Uttar Pradesh

    Directory of Open Access Journals (Sweden)

    Mithila Bisht

    2015-05-01

    Full Text Available Background: Cervical cancer is the most common cancer among females in India. It is also one of the major causes of deaths among them. Papanicolaou (Pap cytological test helps in detecting the early epithelial abnormalities in cervical cells. The morbidity and mortality due to cervical cancer has come down in countries with well-established screening programmes at national level. This study was conducted to emphasize the value of cervical screening as a tool for early detection of non-neoplastic and neoplastic lesions of cervix. Slide positivity rate was computed and clinico-pathological correlation was done. Methods: This cross-sectional study was conducted from January 2012 to December 2014 (3 years, on 2202 women aged between 20-70 years coming for a Pap smear examination in a tertiary care hospital in Bareilly, India. After staining with conventional Papanicolaou technique, all cases were classified as per Bethesda nomenclature (2001. Results: Out of 2202 cases, 85.16% slides were found to be negative for intraepithelial abnormalities and 14.84% slides were labelled as positive for epithelial lesions. 97.67% of high grade lesions were found in women more than 40 years. Smears with epithelial cell abnormality were found more common in older age groups whereas smears negative for epithelial cell abnormality were found more common in young age groups. Conclusion: Pap test is a cost effective cancer screening and is a simple method to detect various lesions of cervix, non-neoplastic as well as neoplastic. High risk screening programmes should be directed to all women >40 years. Pap test is especially useful to diagnose precancerous lesions of cervix, thereby early detection of these lesions and subsequent proper treatment can be helpful in prevention of cervical cancer. [Int J Res Med Sci 2015; 3(5.000: 1070-1076

  15. Human papilloma virus genotypes in women from Nayarit, Mexico, with squamous intraepithelial lesions and cervical cancer

    Science.gov (United States)

    Ortega-Cervantes, Laura; Aguilar-Lemarroy, Adriana; Rojas-García, Aurora Elizabeth; Barrón-Vivanco, Briscia Socorro; Vallejo-Ruiz, Verónica; León, David Cantú-De; Hernández, Yael Yvette Bernal; Jáuregui-Martínez, Armando; Medina-Díaz, Irma Martha

    2016-01-01

    Objective In Mexico cervical cancer (CC) is the most common cause of death from neoplasia in women. Study aimed to analyze the current distribution of Human papillomavirus (HPV) types in women from Nayarit, Mexico, with Squamous intraepithelial lesions (SIL) and Cervical cancer (CC). Methodology Between January 2011 and July 2013, cervical samples were collected from female residents of the Mexican state of Nayarit and were analyzed by means of a LINEAR ARRAY® HPV genotyping test. Data analyses were performed using Stata ver. 8.0 statistical software. Results Of the samples analyzed, 91.2%, HPV DNA was detected. Of these positive samples, 82% were High-risk (HR) viral types. The most prevalent HPV genotypes identified were 16, 58, 31, 18, and 70. Forty two percent of participants had a single infection, while 23 and 26% of participants were infected with two or more HPV genotypes, respectively. HPV 16 was the most prevalent genotype identified and was frequently present as a co-infection with HPV types 18, 51, 52, 59, 66, or 70. Conclusion Women <20 years of age were most often infected with HPV, and the HPV Quadrivalent vaccine (types 16, 18, 6, and 11), currently available in Mexico, no confers protection against a subset of the HPV genotypes identified in the present study (58, 31, 70, and 35). Thus, it is important evaluate the geographical distribution of specific HPV genotypes in all health of center across Mexico in order to implement a successful vaccination program and to diagnose CC in its early stages. PMID:27610056

  16. Motor Deficit After Cervical Surgery, Parsonage-Turner Syndrome or Root Lesion

    Directory of Open Access Journals (Sweden)

    Ayse Sarifakioglu

    2013-06-01

    Full Text Available A 60 years old male patient was admitted to our outpatient clinic with the complaint of severe pain in right arm after cervical stenosis surgery which had been decreased with the formation of progressive motor weakness. Although clinical and physical evaluation was compatable with Parsonage-Turner syndrome (PTS, C5 root involvement has been detected. The patient was given exercise therapy with TENS for analgesia. The patient%u2019s motor weakness progressed and he had difficulties with daily living activities. With the review of literature, we are presenting a case, whose diagnosis was difficult in terms of differentiating both PTS and root lesion which could only be diagnosed by electroneuromyography.

  17. Two-year clinical evaluation of three adhesive systems in non-carious cervical lesions

    Directory of Open Access Journals (Sweden)

    Evrim Eliguzeloglu Dalkilic

    2012-04-01

    Full Text Available OBJECTIVES: Adhesive systems are continuously being introduced to Dentistry, unfortunately often without sufficient clinical validation. The aim of this study was to evaluate the clinical performance of cervical restorations done with three different adhesive systems. MATERIAL AND METHODS: 158 non-carious cervical lesions of 23 patients were restored with a nanofilled composite resin (Filtek Supreme, 3M/ESPE combined with Single Bond (3M/ESPE, group SI, Clearfil SE (Kuraray Medical Inc., group CL and Xeno III (De Trey Dentsply, group XE. In groups SI-B, CL-B and XE-B, the outer surface of the sclerotic dentin was removed by roughening with a diamond bur before application of the respective adhesive systems. In groups CL-BP and XE-BP, after removal of the outer surface of the sclerotic dentin with the bur, the remaining dentin was etched with 37% phosphoric acid and the self-etch adhesive systems Clearfil SE and Xeno III were applied, respectively. Lesions were evaluated at baseline, and restorations after 3 months, 1 year and 2 years using modified USPHS criteria. RESULTS: After 2 years, no significant difference was found between the retention rates of the groups (p >0.05. Although groups CL and SI showed significantly better marginal adaptation than group XE (p0.05. After 2 years no significant difference was observed among the marginal staining results of all groups (p>0.05. CONCLUSION: Although all adhesive systems showed similar retention rates, Clearfil SE and Single Bond showed better marginal adaptation than Xeno III after 2 years of follow-up.

  18. Human papillomavirus types distribution in eastern Sicilian females with cervical lesions. A correlation with colposcopic and histological findings.

    Science.gov (United States)

    Le Donne, Maria; Giuffrè, Giuseppe; Caruso, Carmela; Nicotina, Piero Antonio; Alibrandi, Angela; Scalisi, Rosalba; Simone, Angela; Chiofalo, Benito; Triolo, Onofrio

    2013-07-01

    To determine human papillomavirus (HPV) types distribution in cervical lesions in a Southern Italian female population in Messina and their relationship between HPV type and grade of colposcopic and histopathological abnormality, a total of 253 women aged 17-68 years, with previous cytological abnormalities, were included in this study. HPV-DNA testing, colposcopy and biopsy were performed. For each sample, cervical cells were collected by centrifugation and DNA was extracted, followed by a PCR-based HPV-DNA assay and reverse dot blot genotyping. HPV-16 was found the most common type (46.6 %) followed by HPV-31 (26.9 %), -6 (18.6 %), -58 (8.8 %), -18 (6.7 %), -66 (5.7 %), -52 and -53 (4.7 %). Out of 62 women with abnormal transformation zone (ATZ) area compatible with squamous intraepithelial lesion (SIL) or cervical cancer (CC), 64.5 % was found high risk (HR) HPV-positive. Moreover the severity of the colposcopic diagnosis was positively correlated with the higher HPV oncogenicity risk (HPV-16 P = 0.023; and HPV-53 P = 0.047). The HPV-16 was found the most prevalent type within each histological category: 66.7 %, 31.2 %, 44 % and 37.2 % of CC, high grade (H)SIL, low grade (L)SIL and chronic cervicitis respectively; followed by HPV-31 present in 25 %, 8 %, and 13.3 % of HSIL, LSIL and chronic cervicitis respectively. A higher HPV incidence than the rest of Italy was found, in agreement with that detected by other authors for the South of the country. These data provide further information about the types prevalence in women with cervical lesions living in Eastern Sicily, suggesting the introduction of new targeted vaccines against a wider spectrum of HPV.

  19. Associated factors with cervical pre-malignant lesions among the married fisher women community at Sadras, Tamil Nadu

    Directory of Open Access Journals (Sweden)

    Sornam Ganesan

    2015-01-01

    Full Text Available Objective: To identify the associated factors of cervical pre-malignant lesions among the married fisher women residing in the coastal areas of Sadras, Tamil Nadu. Methods: The study was conducted in five fishermen communities under Sadras, a coastal area in Tamil Nadu, India. Two hundred and fifty married fisher women residing in the area. Quantitative descriptive approach with a cross-sectional study design was used. Data were collected using a structured interview schedule for identifying the associated factors and Pap smear test was performed for identifying the pre-malignant cervical lesions among the married fisher women. Data were analyzed using descriptive and inferential statistics. Results: Among 250 women, about six (2.4% of them presented with pre-cancerous lesions such as atypical squamous cell of undifferentiated significance (ASCUS - five (2% and mild dysplasia one (0.4%. Majority of the women, about 178 (71.2% women, had abnormal cervical findings. Statistical analysis showed a significant association of risk factors such as advanced age, lack of education, low socioeconomic status, using tobacco, multiparity, premarital sex, extramarital relationship, using cloth as sanitary napkin, etc. Conclusion: The study findings clearly show the increased vulnerable state of the fisher women for acquiring cervical cancer as they had many risk factors contributing to the same.

  20. Incidence of cervical lesions in Danish women before and after implementation of a national HPV vaccination program

    DEFF Research Database (Denmark)

    Baldur-Felskov, Birgitte; Dehlendorff, Christian; Junge, Jette;

    2014-01-01

    PURPOSE: Approximately 7% of cervical cancers and about 50% of high-grade cervical precursor lesions are caused by human papillomavirus (HPV) types 16 and 18. Denmark introduced the quadrivalent HPV vaccine into the vaccination program for 12-year-old girls in 2009 supplemented by a first catch......+) and cervical intraepithelial neoplasia grade 2 or worse (CIN2+) increased in all age groups in 2000-2010. After introduction of the quadrivalent HPV vaccine into the vaccination program, the incidence of atypia+ decreased significantly in women younger than 18 years (EAPC -33.4%; 95% CI -49.6; -12.0) and in 18...... in age groups with high HPV vaccine coverage, indicating an early effect of HPV vaccination....

  1. Differential methylation of E2 binding sites in episomal and integrated HPV 16 genomes in preinvasive and invasive cervical lesions.

    Science.gov (United States)

    Chaiwongkot, Arkom; Vinokurova, Svetlana; Pientong, Chamsai; Ekalaksananan, Tipaya; Kongyingyoes, Bunkerd; Kleebkaow, Pilaiwan; Chumworathayi, Bandit; Patarapadungkit, Natcha; Reuschenbach, Miriam; von Knebel Doeberitz, Magnus

    2013-05-01

    Enhanced expression of the HPV 16 E6-E7 oncogenes may trigger neoplastic transformation of the squamous epithelial cells at the uterine cervix. The HPV E2 protein is a key transcriptional regulator of the E6-E7 genes. It binds to four E2 binding sites (E2BSs 1-4) in the viral upstream regulatory region (URR). Modification of E2 functions, for example, by methylation of E2BSs is hypothesized to trigger enhanced expression of the viral E6-E7 oncogenes. In the majority of HPV-transformed premalignant lesions and about half of cervical carcinomas HPV genomes persist in an extra-chromosomal, episomal state, whereas they are integrated into host cells chromosomes in the remaining lesions. Here we compared the methylation profile of E2BSs 1-4 of the HPV 16 URR in a series of 18 HPV16-positive premalignant lesions and 33 invasive cervical cancers. CpGs within the E2BSs 1, 3, and 4 were higher methylated in all lesions with only episomal HPV16 genomes compared with lesions displaying single integrated copies. Samples with multiple HPV16 integrated copies displayed high methylation levels for all CpGs suggesting that the majority of multiple copies were silenced by extensive methylation. These data support the hypothesis that differential methylation of the E2BSs 1, 3 and 4 is related to the activation of viral oncogene expression in cervical lesions as long as the viral genome remains in the episomal state. Once the virus becomes integrated into host cell chromosomes these methylation patterns may be substantially altered due to complex epigenetic changes of integrated HPV genomes.

  2. Restoration of non-carious cervical lesions with ceramic inlays: A possible model for clinical testing of adhesive cements

    OpenAIRE

    Michal Staninec; Tsuji, Grant H.

    2012-01-01

    Introduction: There are many luting cements coming to market which claim to be adhesive, but there is no clinical protocol currently for testing these claims. There is a standardized protocol for testing direct restorations bonded to dentin and it is used extensively. Case Report: We describe a clinical procedure for restoring a non-carious cervical lesion (NCCL) with a ceramic inlay using Computer-Aided Design and Computer-Aided Manufacturing (CAD-CAM) technology and an adhesive resin cement...

  3. Cervical pre-malignant lesions in HIV infected women attending Care and Treatment Centre in a tertiary hospital, Dar es Salaam, Tanzania.

    Science.gov (United States)

    Balandya, Belinda S; Pembe, Andrea B; Mwakyoma, Henry A

    2011-09-01

    The aims of this study was to determine proportion of HIV infected women with cervical pre-malignant lesions; and compare the use of Visual Inspection of the cervix after application of Acetic acid (VIA) and Papanicolau (Pap) smear in screening for cervical premalignant lesions in HIV positive women attending Care and Treatment Centre (CTC) at Muhimbili National Hospital (MNH), Dar es Salaam, Tanzania. A total of 316 women aged 18-70 years had a Pap smear taken for cytology, followed by spraying onto the cervix with 4% acetic acid and then inspecting it. Cytology was considered negative when there was no Cervical Intraepithelial Neoplasia (CIN) lesion reported from the Pap smear taken, and positive if CIN lesion 1, 2 or 3 was reported. Detection of a well-defined, opaque acetowhite lesion close to the squamocolumnar junction or close to the external cervical os constituted a positive VIA. Out of 316 women, 132 women had acetowhite lesions on VIA, making the proportion of abnormal cervical lesions to be 42.4%. One hundred and one out of 312 women (32.4%) had CIN lesions detected on Pap smear. The proportion of agreement between these two tests was 0.3. The proportion of agreement was moderate in women with advanced WHO HIV clinical stage of the disease and in women not on ART (Anti Retroviral Therapy). Women with CD-4 count less than 200 cells/mm3 had more abnormal cervical lesions. There is considerable proportion of HIV positive women with premalignant lesions of the cervix. Considering the proportion of HIV women with abnormal lesions and the difficulty in logistics of doing Pap smear in low resource settings, these results supports the recommendation to introduce screening of premalignant lesions of the cervix using VIA to all HIV infected women.

  4. Immunohistochemical Expression of VEGF and Podoplanin in Uterine Cervical Squamous Intraepithelial Lesions

    Science.gov (United States)

    Belfort-Mattos, Patrícia Napoli; Focchi, Gustavo Rubino de Azevedo; Ribalta, Julisa Chamorro Lascasas; Megale De Lima, Tatiana; Nogueira Carvalho, Carmen Regina; Kesselring Tso, Fernanda; De Góis Speck, Neila Maria

    2016-01-01

    VEGF and podoplanin (PDPN) have been identified as angiogenesis and/or lymphangiogenesis regulators and might be essential to restrict tumor growth, progression, and metastasis. In the present study, we evaluate the association between the expression of these markers and CIN grade. Immunohistochemistry was performed in 234 uterine cervical samples using conventional histologic sections or TMA with the monoclonal antibodies to VEGF (C-1 clone) and podoplanin (D2-40 clone). Positive-staining rates of VEGF in 191 CIN specimens were significantly associated with histological grade (P < 0.001). Negative and/or focal immunostaining for PDPN were more frequent in CIN 3 (P = 0.016). We found that patients with CIN 3 more frequently had strong and more diffuse staining for VEGF and diminished staining for PDPN (P = 0.018). Strong and more diffuse VEGF immunoexpressions in CIN 2 and CIN 3 were detected when compared to CIN 1. Negative and/or focal PDPN immunoexpression appear to be more frequent in CIN 3. Moderate to strong VEGF expression may be a tendency among patients with high-grade lesions and diminished PDPN expression. PMID:27313335

  5. CCR2 and CCR5 genes polymorphisms in women with cervical lesions from Pernambuco, Northeast Region of Brazil: a case-control study.

    Science.gov (United States)

    Santos, Erinaldo Ubirajara Damasceno dos; Lima, Géssica Dayane Cordeiro de; Oliveira, Micheline de Lucena; Heráclio, Sandra de Andrade; Silva, Hildson Dornelas Angelo da; Crovella, Sergio; Maia, Maria de Mascena Diniz; Souza, Paulo Roberto Eleutério de

    2016-03-01

    Polymorphisms in chemokine receptors play an important role in the progression of cervical intraepithelial neoplasia (CIN) to cervical cancer (CC). Our study examined the association of CCR2-64I (rs1799864) andCCR5-Δ32 (rs333) polymorphisms with susceptibility to develop cervical lesion (CIN and CC) in a Brazilian population. The genotyping of 139 women with cervical lesions and 151 women without cervical lesions for the CCR2-64I and CCR5-Δ32 polymorphisms were performed using polymerase chain reaction-restriction fragment length polymorphism. The individuals carrying heterozygous or homozygous genotypes (GA+AA) for CCR2-64I polymorphisms seem to be at lower risk for cervical lesion [odds ratio (OR) = 0.37, p = 0.0008)]. The same was observed for the A allele (OR = 0.39, p = 0.0002), while no association was detected (p > 0.05) with CCR5-Δ32 polymorphism. Regarding the human papillomavirus (HPV) type, patients carrying the CCR2-64Ipolymorphism were protected against infection by HPV type 16 (OR = 0.35, p = 0.0184). In summary, our study showed a protective effect ofCCR2-64I rs1799864 polymorphism against the development of cervical lesions (CIN and CC) and in the susceptibility of HPV 16 infection.

  6. Cervical Lesions in Women Younger than 25 Years Lesiones de cuello uterino en mujeres menores de 25 años

    Directory of Open Access Journals (Sweden)

    Yoleisi Alonso Fleites

    2011-12-01

    Full Text Available

    Background: Cervical cancer is essentially a sexually transmitted disease related to human papillomavirus infection. This disease’s incidence has been globally enhanced, particularly in increasingly younger women. Objective: To determine cervical lesions in women younger than 25 years. Methods: An observational, descriptive and correlational study including 248 patients registered at the Pathological Anatomy Service of the General University Hospital Dr. Gustavo Aldereguía Lima of Cienfuegos was conducted from January to December 2007. It also included the first quarter of 2008. The following variables were analyzed: patient’s age, age at first intercourse, number of sexual partners, type and degree of injury, and reason for attending consultation. Results: Women over 21 years old and those who had had 2 and 3 sexual partners predominated. The main reason for attending consultation was cervicitis (84, 7%. In 22.6% of women grade I intraepithelial neoplasia was detected, while grade II and III were detected in 41, 9% and 33, 1%, respectively. In 90% of younger patients the lesion was malignant. Conclusions: The presence of cervical lesions in women under 25 years is not low and neither is the frequency of malignant lesions.

    Fundamento: el cáncer cervical es esencialmente una enfermedad de transmisión sexual relacionada con la infección por el virus del papiloma humano. Este fenómeno ha aumentado globalmente, en especial en mujeres cada vez más jóvenes. Objetivo: determinar las lesiones de cuello uterino en mujeres menores de 25 años. Métodos: estudio observacional, descriptivo y correlacional que incluyó 248 pacientes registradas en el Servicio de Anatomía Patológica del Hospital General Universitario Dr. Gustavo Aldereguía Lima de Cienfuegos, en el periodo de enero a

  7. 围绝经期宫颈病变所致阴道流血%Perimenopausal abnormal vaginal bleeding caused by cervical lesions

    Institute of Scientific and Technical Information of China (English)

    王登凤; 张国楠

    2012-01-01

    围绝经期妇女中,不规则阴道流血是一个最常见、也是最易被忽视的症状,宫颈病变是其主要原因之一,主要包括了宫颈炎、宫颈炎症相关疾病、宫颈上皮内瘤变、宫颈癌、宫颈肌瘤、宫颈结核、宫颈妊娠等,可以通过妇科查体、宫颈脱落细胞学检查、宫颈活检等简单手段在短时间内得到确诊.%For perimenopausal women, abnormal vaginal bleeding is the most common symptom, which is also the most neglected one. In various causes, cervical lesion is the one which can be diagnosed by some simple methods, e. g. gynecological examination , cervical cytology, and cervical biopsy. Cervical lesions in this review include: cervicitis, cervicitis related disease, cervical intraepithelial neoplasia, cervical cancer, cervical myoma, tuberculosis of cervix, cervical pregnancy, etc.

  8. Cervical myelopathy due to single level disc herniation presenting as intramedullary mass lesion: What to do first?

    Science.gov (United States)

    Ekşi, Murat Şakir; Özcan Ekşi, Emel Ece; Yılmaz, Baran; Toktaş, Zafer Orkun; Konya, Deniz

    2015-01-01

    Cervical myelopathy (CM) is mostly a degenerative process ending in myelopathic and/or radiculopathic syndromes. On T2-weighted magnetic resonance imaging (MRI), CM appears as a hyperintense area near the spondylotic spine. This high intensity signal depends on the impact of outer forces and their duration. It also determines the prognosis of the surgical candidate. A 40-year-old male patient admitted to our clinic with right upper extremity weakness and hypoesthesia that had started 2 months earlier. On neurological examination there was 2/5 motor weakness of right biceps brachii, and hypoesthesia over right C6 dermatome. Right upper extremity deep tendon reflexes were hypoactive, but lower ones were hyperactive. After clinical and radiological work-up, preliminary diagnosis was directed to a spinal intramedullary tumor. Total resection of the herniated cervical disc fragment and the mass lesion was managed. Pathology of the mass lesion was compatible with subacute infarct tissue and inflammatory response. Final diagnosis was CM under effect of cervical disc herniation. Contrast-enhanced spinal cord myelopathic lesions are very rare and resemble much more tumors and inflammatory processes. However, the principal treatment approach totally differs depending on pathology. When there are both a disc herniation and a high clinical suspicion; biopsy should be delayed. The most probable solution will be surgery for the disc disease with thorough preoperative scanning of vascular malformations; clinical and radiological close follow-up after surgery. Biopsy or surgical resection can be performed if patient deteriorates despite the primary surgery. PMID:25972718

  9. HPV genotypes in high grade cervical lesions and invasive cervical carcinoma as detected by two commercial DNA assays, North Carolina, 2001-2006.

    Directory of Open Access Journals (Sweden)

    Susan Hariri

    Full Text Available BACKGROUND: HPV typing using formalin fixed paraffin embedded (FFPE cervical tissue is used to evaluate HPV vaccine impact, but DNA yield and quality in FFPE specimens can negatively affect test results. This study aimed to evaluate 2 commercial assays for HPV detection and typing using FFPE cervical specimens. METHODS: Four large North Carolina pathology laboratories provided FFPE specimens from 299 women ages18 and older diagnosed with cervical disease from 2001 to 2006. For each woman, one diagnostic block was selected and unstained serial sections were prepared for DNA typing. Extracts from samples with residual lesion were used to detect and type HPV using parallel and serial testing algorithms with the Linear Array and LiPA HPV genotyping assays. FINDINGS: LA and LiPA concordance was 0.61 for detecting any high-risk (HR and 0.20 for detecting any low-risk (LR types, with significant differences in marginal proportions for HPV16, 51, 52, and any HR types. Discordant results were most often LiPA-positive, LA-negative. The parallel algorithm yielded the highest prevalence of any HPV type (95.7%. HR type prevalence was similar using parallel (93.1% and serial (92.1% approaches. HPV16, 33, and 52 prevalence was slightly lower using the serial algorithm, but the median number of HR types per woman (1 did not differ by algorithm. Using the serial algorithm, HPV DNA was detected in >85% of invasive and >95% of pre-invasive lesions. The most common type was HPV16, followed by 52, 18, 31, 33, and 35; HPV16/18 was detected in 56.5% of specimens. Multiple HPV types were more common in lower grade lesions. CONCLUSIONS: We developed an efficient algorithm for testing and reporting results of two commercial assays for HPV detection and typing in FFPE specimens, and describe HPV type distribution in pre-invasive and invasive cervical lesions in a state-based sample prior to HPV vaccine introduction.

  10. TCT联合HPV检测在宫颈病变筛查中的意义%Cervical Liquid-Based Cytology Combined Human Papillomavirus Testing in Cervical Lesion

    Institute of Scientific and Technical Information of China (English)

    戴一菲; 田荣华

    2012-01-01

    Objective To study the significance of combination of cervical liquid-based cytology and human papillomavirus detection in cervical lesion screening. Methods From March 2010 to December 2011,3,028 patients were detected with nipple shaped tumor virus ( HPV) and cervical liquid base cytology (TCT) ,TCT was used by 2001 International Cancer Association TBS diagnosis system report way,to the patients with positive results of TCT check for ASCIIS and the above lesions and (or) HPV detection, cervical organization biopsy under electronic vaginal mirror were performed. Results The sensitivity of TCT check was 56.72% ,with the specificity of 28. 19% ;the sensitivity of HPV check was 86.03% ,with specific for 46.38% ;among 408 cases of CIN and cervical cancer pathology ,28 cases were single TCT positive (6. 86% ) ;with TCT and HPV positive 203 ca-ses(49. 75% ) ,there was significant difference in diagnosis of cervical squamous intraepithelial neoplasm ( CIN) and cervical cancer(x2 = 184.926,P=0.000) between single TCT check and combination of TCT and HPV examination. Conclusion TCT combined with HPV check can improve the detection rate of cervical cancer and precancerous lesions, improve the sensitivity and specificity of screening,effectively reduce the TCT in detection of false-negative rates,and avoid excessive colposcopy and biopsy in low-risk populations and over-treatment.%目的 探讨宫颈液基细胞学检查与人乳头状瘤病毒检测联合应用在宫颈病变筛查中的意义.方法 选取2010年3月-2011年12月孝感市中心医院病理科同时进行了宫颈液基细胞学(TCT)检查及人乳头状瘤病毒(HPV)检测的患者3028人,TCT采用2001年国际癌症协会TBS诊断系统报告方式,对TCT检查结果为ASCUS及以上病变和(或)HPV检测结果为阳性的患者在电子阴道镜下取宫颈组织活检.结果 TCT检查敏感度为56.72%,特异度为28.19%;HPV检查敏感度为86.03%,特异度为46.38%;病

  11. DAPK1, MGMT and RARB promoter methylation as biomarkers for high-grade cervical lesions

    OpenAIRE

    Sun, Yin; Li, Shu; Shen, Keng; Ye, Shuang; Cao, Dongyan; Yang, Jiaxin

    2015-01-01

    Gene promoter methylation may be used a potential biomarker for detecting solid tumor including cervical cancer. Here, we used methylation sensitive-high resolution melting (MS-HRM) analysis to detecting promoter methylation ratios of DAPK1, MGMT and RARB gene in patients with different cervical disease grade. The detection of gene promoter methylation was conducted in two hundred fifty patients’ samples including normal cytology (n=48), cervical intraepithelial neoplasia grade 1 (CIN1, n=54)...

  12. DAPK1, MGMT and RARB promoter methylation as biomarkers for high-grade cervical lesions

    Science.gov (United States)

    Sun, Yin; Li, Shu; Shen, Keng; Ye, Shuang; Cao, Dongyan; Yang, Jiaxin

    2015-01-01

    Gene promoter methylation may be used a potential biomarker for detecting solid tumor including cervical cancer. Here, we used methylation sensitive-high resolution melting (MS-HRM) analysis to detecting promoter methylation ratios of DAPK1, MGMT and RARB gene in patients with different cervical disease grade. The detection of gene promoter methylation was conducted in two hundred fifty patients’ samples including normal cytology (n=48), cervical intraepithelial neoplasia grade 1 (CIN1, n=54), cervical intraepithelial neoplasia grade 2 (CIN2, n=47), cervical intraepithelial neoplasia grade 3 (CIN3, n=56) and cervical squamous cell carcinomas (SCS, n=45). We found there were a significant positive correlation between the promoter methylation status of DAPK1 and cervical disease grade (P=0.022). In addition, the methylated promoters of DAPK1 combined with MGMT, MGMT combined with RARB, DAPK1 combined with RARB were positive correlated with cervical disease grade (P < 0.05). All three genes promoters methylated were positive correlated with cervical disease grade (P < 0.001). Receiver operating characteristic (ROC) curves was conducted to evaluate whether the three genes methylation could be used to be a potential marker for diagnosing high grade cervical disease (HSIL and SCC). The cutoff values for the methylation rates of all these genes were 0-5%. Regrettably, only the methylation of MGMT combined with DAPK1 gave 43.4% sensitivity and 68.6% specificity. The current results indicated that MS-HRM-based testing for DNA methylations of MGMT plus DAPK1 genes holds some promise for high grade cervical disease screening. PMID:26823825

  13. Randomized clinical trial of four adhesion strategies in cervical lesions: 12-month results.

    Science.gov (United States)

    de Paula, Eloisa Andrade; Tay, Lidia Yileng; Kose, Carlos; Mena-Serrano, Alexandra; Reis, Alessandra; Perdigão, Jorge; Loguercio, Alessandro D

    2015-01-01

    The aim of this study was to evaluate the 6- and 12-month clinical performance of four adhesion strategies from the same manufacturer (Kerr) in non-carious cervical lesions (NCCLs) using two evaluation criteria. Thirty-five patients, with at least four NCCLs each, participated in this study. After samplesize calculation, 180 restorations were assigned to one of the following groups: OFL (Optibond FL), OSP (Optibond Solo Plus), XTR (Optibond XTR), and AIO (Optibond All-In-One). The composite resin Filtek Supreme Ultra (3M ESPE) was placed incrementally. The restorations were evaluated at baseline, after 6 months, and after 12 months, using both the FDI and the USPHS-modified criteria. Statistical analyses were performed with Friedman repeated measures, ANOVA by rank, and the McNemar test for significance in each pair (α=0.05). Six restorations (2 for OFL, 1 for OSP, 2 for XTR, and 1 for AIO) were lost at 12 months (P>0.05 for both evaluation criteria). Marginal staining was observed in seven restorations using the FDI criteria (P>0.05) and three restorations using the USPHSmodified criteria (P>0.05). Eight restorations (2 for OSP, 3 for XTR, and 3 for AIO) were classified as Bravo for marginal adaptation using the USPHSmodified criteria (P>0.05). However, 62 restorations (14 for OFL, 12 for OSP, 15 for XTR, and 21 for AIO) were classified as Bravo using the FDI criteria (P>0.05). The four adhesion strategies showed similar clinical retention at 6 and 12 months. The FDI evaluation criteria tend to be more sensitive than the USPHS-modified criteria. PMID:25625130

  14. The Class V lesion--aetiology and restoration.

    Science.gov (United States)

    Tyas, M J

    1995-06-01

    The aetiology of non-carious cervical lesions is discussed. These have been variously described as 'abrasion', 'erosion', 'abrasion/erosion', and 'idiopathic cervical'. However, many lesions do not fit the classical appearance or location of an erosive and/or abrasive origin, and there is increasing interest in the possible role of occlusal stress in their aetiology. Non-carious cervical lesions often require restoration, and there are essentially three options using tooth-coloured materials: a restorative glass ionomer cement, a liner/base glass ionomer cement overlayed with a resin composite, or a resin composite bonded by an enamel/dentine-adhesive. The materials and techniques used in these options are discussed, indicating their advantages and disadvantages. Results of available clinical trials of these systems are given, and the link with the stress theory of cervical tooth loss is described. The preferred restorative approaches in order are: resin-modified restorative glass ionomer; resin-modified liner/base glass ionomer with a microfine resin composite overlay; enamel/dentine bonding agent with a microfine resin composite.

  15. Clinical and epidemiological correlations between the infection with HPV 16 and HPV 18 and female cervical lesions.

    Science.gov (United States)

    Stoian, M; Repanovici, R; Corniţescu, F

    1995-01-01

    A number of 66 specimens from female cervical lesions were examined for infection with human papillomavirus (HPV) types 6, 11, 16, and 18 by nucleic acid hybridization in dot-blot techniques and 35 sera were tested by the immunodot-blot technique, in order to detect the presence of anti E4 and E7 HPV protein antibodies. The findings were compared with the histologic diagnosis. Fifty-six per cent of specimens contained HPV DNA sequences. In 47% of specimens from cervical carcinoma, HPV 11 was detected in 4 cases, HPV 16 in 21 cases, and HPV 18 in 7 cases. Serum antibodies against HPV 16 E4 and HPV 16 E7 occurred in all the cases of uterine carcinoma, in 4 of 10 cases of CIN I-II, and in 3 of 5 sera obtained from apparently healthy women. The analysis of risk factors disclosed the early onset of sexual activity, a relatively high number of births and abortions before the age of 22 years, the use of oral oestroprogestative contraceptive agents, the presence in anamnesis of genital infections with bacterial flora--Candida albicans, Trichomonas vaginalis, Chlamydia trachomatis, Mycoplasma, etc. Our results showed that HPV typing by nucleic acid hybridization was useful for differentiating low- from high-risk cervical lesions and also tried to elucidate the risk factors associated with HPV infections and progression to malignancy. PMID:9179967

  16. HPV prevalence and type distribution in women with or without cervical lesions in the Northeast region of Romania

    Directory of Open Access Journals (Sweden)

    Ursu Ramona

    2011-12-01

    Full Text Available Abstract Background Cervical cancer is a major public health problem worldwide. While Romania has the highest incidence of cervical cancer in Europe, the prevalence of HPV has not been evaluated. We report the first data on HPV prevalence and type distribution in Northeast Romania. Methods HPV prevalence and genotype distribution was investigated in 514 consecutively women with or without cervical lesions in Northeast Romania. Genotyping was performed with Linear Array Genotyping/Roche kit. Results In our study group, 192/514 (37.4% patients were positive for HPV (infected with single and with multiple HPV types. Most frequent types were: 16 (10.5%, 53 (5.44%, 51 (5.05%, 52 (4.08% 18 (2.91% and 31 (2.73%. Conclusions Infection with high risk types of HPV is common in Northeast Romania. Enhanced and systematic screening for cervical cancer is needed. Our results call for the implementation of a National HPV vaccine program in Romania.

  17. Methylation and expression of miRNAs in precancerous lesions and cervical cancer with HPV16 infection.

    Science.gov (United States)

    Jiménez-Wences, Hilda; Martínez-Carrillo, Dinorah Nashely; Peralta-Zaragoza, Oscar; Campos-Viguri, Gabriela Elizabeth; Hernández-Sotelo, Daniel; Jiménez-López, Marco Antonio; Muñoz-Camacho, José Guadalupe; Garzón-Barrientos, Víctor Hugo; Illades-Aguiar, Berenice; Fernández-Tilapa, Gloria

    2016-04-01

    Abnormal expression and promoter methylation of microRNAs (miRNAs) are common events during cervical carcinogenesis. Worldwide, infection by types 18 and 16 of human papillomaviruses (HPVs) is considered the major risk factor for cervical cancer development. It has been reported that expression of the miRNAs can be deregulated by specific HPV genotypes. In this study we analyzed the promoter methylation of 22 miRNAs and the expression of three miRNAs in 10 non-squamous intraepithelial lesions (Non-SIL) without HPV16 infection, and 7 Non-SIL, 16 low-grade SIL (LSIL) and 16 cervical cancer samples, all with HPV16 infection. The methylation status was determined using Human Cancer miRNA EpiTect Methyl II Signature PCR Array® and the expression of miR-124, miR-218 and miR-193b was determined by qRT-PCR using individual TaqMan assays. Comparisons of groups defined were performed using the Fisher exact test for categorical variables and Mann-Whitney test for continuous variables. A p-value of <0.05 was considered statistically significant. The methylation levels of miR-124-2, miR-218-1, miR-218-2 and miR-34b/c promoters were significantly higher in cervical cancer than in LSIL samples. The methylation levels of miR-193b promoter were significantly lower in cervical cancer than in LSIL samples. The expression of miR-124 and miR-218 was significantly lower in cervical cancer than in LSIL samples. The expression of miR-193b was significantly higher in cervical cancer than in LSIL and Non-SIL samples. Our results suggest that the abnormal promoter methylation and expression of miR-124, miR-218 and miR-193b are common events during cervical carcinogenesis. PMID:26797462

  18. Quadrivalent HPV vaccine effectiveness against high-grade cervical lesions by age at vaccination: A population-based study.

    Science.gov (United States)

    Herweijer, Eva; Sundström, Karin; Ploner, Alexander; Uhnoo, Ingrid; Sparén, Pär; Arnheim-Dahlström, Lisen

    2016-06-15

    Human papillomavirus (HPV) types 16/18, included in HPV vaccines, contribute to the majority of cervical cancer, and a substantial proportion of cervical intraepithelial neoplasia (CIN) grades 2/3 or worse (CIN2+/CIN3+) including adenocarcinoma in situ or worse. The aim of this study was to quantify the effect of quadrivalent HPV (qHPV) vaccination on incidence of CIN2+ and CIN3+. A nationwide cohort of girls and young women resident in Sweden 2006-2013 and aged 13-29 (n = 1,333,691) was followed for vaccination and histologically confirmed high-grade cervical lesions. Data were collected using the Swedish nationwide healthcare registers. Poisson regression was used to calculate incidence rate ratios (IRRs) and vaccine effectiveness [(1-IRR)x100%] comparing fully vaccinated with unvaccinated individuals. IRRs were adjusted for attained age and parental education, and stratified on vaccination initiation age. Effectiveness against CIN2+ was 75% (IRR = 0.25, 95%CI = 0.18-0.35) for those initiating vaccination before age 17, and 46% (IRR = 0.54, 95%CI = 0.46-0.64) and 22% (IRR = 0.78, 95%CI = 0.65-0.93) for those initiating vaccination at ages 17-19, and at ages 20-29, respectively. Vaccine effectiveness against CIN3+ was similar to vaccine effectiveness against CIN2+. Results were robust for both women participating to the organized screening program and for women at prescreening ages. We show high effectiveness of qHPV vaccination on CIN2+ and CIN3+ lesions, with greater effectiveness observed in girls younger at vaccination initiation. Continued monitoring of impact of HPV vaccination in the population is needed in order to evaluate both long-term vaccine effectiveness and to evaluate whether the vaccination program achieves anticipated effects in prevention of invasive cervical cancer.

  19. Cervical myelopathy due to single level disc herniation presenting as intramedullary mass lesion: What to do first?

    Directory of Open Access Journals (Sweden)

    Murat Sakir Eksi

    2015-01-01

    Full Text Available Cervical myelopathy (CM is mostly a degenerative process ending in myelopathic and/or radiculopathic syndromes. On T2-weighted magnetic resonance imaging (MRI, CM appears as a hyperintense area near the spondylotic spine. This high intensity signal depends on the impact of outer forces and their duration. It also determines the prognosis of the surgical candidate. A 40-year-old male patient admitted to our clinic with right upper extremity weakness and hypoesthesia that had started 2 months earlier. On neurological examination there was 2/5 motor weakness of right biceps brachii, and hypoesthesia over right C6 dermatome. Right upper extremity deep tendon reflexes were hypoactive, but lower ones were hyperactive. After clinical and radiological work-up, preliminary diagnosis was directed to a spinal intramedullary tumor. Total resection of the herniated cervical disc fragment and the mass lesion was managed. Pathology of the mass lesion was compatible with subacute infarct tissue and inflammatory response. Final diagnosis was CM under effect of cervical disc herniation. Contrast-enhanced spinal cord myelopathic lesions are very rare and resemble much more tumors and inflammatory processes. However, the principal treatment approach totally differs depending on pathology. When there are both a disc herniation and a high clinical suspicion; biopsy should be delayed. The most probable solution will be surgery for the disc disease with thorough preoperative scanning of vascular malformations; clinical and radiological close follow-up after surgery. Biopsy or surgical resection can be performed if patient deteriorates despite the primary surgery.

  20. Metabonomic signature analysis of cervical carcinoma and precancerous lesions in women by 1H NMR spectroscopy

    OpenAIRE

    Hasim, Ayshamgul; ALI, MAYINUER; MAMTIMIN, BATUR; Ma, Jun-Qi; Li, Qiao-Zhi; ABUDULA, ABULIZI

    2012-01-01

    1H nuclear magnetic resonance (NMR)-based metabonomics has been used to characterize the metabolic profiles of cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CSCC). Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used to model the systematic variation related to patients with CIN or CSCC with healthy controls. Potential metabolic biomarkers were identified using database comparisons, and the one-way a...

  1. Prevalence and risk factors for non-carious cervical lesions in children attending special needs schools in India.

    Science.gov (United States)

    Kumar, Sandeep; Kumar, Arunoday; Debnath, Nitai; Kumar, Amit; K Badiyani, Bhumika; Basak, Debashish; S A Ali, Mohamed; B Ismail, Mohammed

    2015-03-01

    We assessed the prevalence and risk factors for development of non-carious cervical lesions (NCCLs) in children attending special needs schools in India. The participants were 395 children aged 12-15 years with disabilities in learning, communication, physical function, and/or development. A questionnaire was designed in order to collect information on sociodemographic characteristics, oral hygiene practices, dietary habits, and risk factors for NCCLs. The chi-square test, bivariate analysis, and logistic regression analysis were used to analyze the data. Presence of NCCLs was associated with use of toothpowder or other materials for teeth cleaning, use of harder toothbrushes, use of a horizontal scrub technique for toothbrushing, consumption of a vegetarian diet, and greater consumption of lemon. The overall prevalence of NCCLs was 22.7%. Most lesions involved minimal loss of contour or defects health care among children attending special needs schools. PMID:25807907

  2. Quality of life in women with cervical precursor lesions and cancer:a prospective, 6-month, hospital-based study in China

    Institute of Scientific and Technical Information of China (English)

    Zhi-Mei Zhao; Xiong-Fei Pan; Si-Han Lv; Yao Xie; Shao-Kai Zhang; You-Lin Qiao; Xiao-Rong Qi; Chun-Xia Yang; Fang-Hui Zhao

    2014-01-01

    The overal survival of patients with cervical cancer has improved due to detection at an early stage and availability of comprehensive treatments in China. As patients’ lives prolonged, it is important to understand their health-related quality of life (QoL) during and after treatment. We used the EQ-5D questionnaire to assess QoL of 194 patients with cervical lesions at Sichuan University West China Second Hospital between May 2010 and January 2011. Patients were surveyed before primary treatment and at 1, 3, and 6 months after primary treatment. Results showed a consistent decline in EQ-5D scores in the spectrum of cervical lesions at each time point after treatment (alP < 0.05). For patients with precursor lesions, there was an increasing trend along the timeline of treatment (P < 0.01). For patients with early-stage cervical cancer, EQ-5D scores declined in the first month (P = 0.01) and gradualy increased to higher levels at 6 months post-treatment than those before treatment (P < 0.01). EQ-5D scores followed a similar trend in patients with advanced cervical cancer (P = 0.04), though they did not statisticaly rebound after 6 months (0.84± 0.19 vs. 0.86± 0.11,P = 0.62). Regarding advanced cervical cancer, EQ-5D scores for women above 40 years of age appeared to recover more rapidly and reached higher levels than those for women below 40 years (P = 0.03). Caution and extra care are recommended in the early period of cervical cancer treatment given the slight deterioration in the QoL, and in particular, for younger cervical cancer patients. Our study implies that health care providers may need to improve the health-related QoL of cervical cancer patients.

  3. Prevalence of high-risk human papillomavirus infection in different cervical lesion among organized health-examination women in Shanghai, China

    Institute of Scientific and Technical Information of China (English)

    ZHANG Wen-ying; XUE Yue-zhen; CHEN Min; HAN Ling; LUO Man

    2008-01-01

    Background Cervical cancer is the second most common cause of death from cancer among women worldwide.Human papillomavirus(HPV)plays a central role in the etiology of cervical cancer.It is important to describe the prevalence of HPV infection in different types of cervical lesions and to explore the relation between HPV viral load and the severity of cervical lesions.Methods To describe the HPV infection prevalence and viral load in different age groups,we retrospectively investigated 6405 cases of women who were organized by their units to take health-examination.They were given Hybrid Capture ll tests between Janury 2005 and December 2006.The correlation between HPV viral load and pathology was assessed.Results Overall HPV infection prevalence was 29.1%(1864/6405),while in women 18-20 years old it was 54.4% (31/57),the highest among all age groups.After declining rapidly,HPV prevalence stabilized at about 30.0%in women aged 30 and older.Of the 6405 women,1 483 women had a colposcopic biopsy and 33.2%(492/1 483)were positive for HPV DNA.Twenty-one percent of women with a normal diagnosis(238/1 095)had HPV infection,a statistically significantly lower prevalence than in women with cervical lesions,including those with cervical intraepithelial neoplasia (68.8%in CINl,66.7% in CIN2,and 76.5%in CIN3)or with cervical cancer(94.1%).The correlation coefficient between viral load and cervical lesion severity was 0.134,which was not tatistically significant(P=0.075).Viral load values in women with CINs and cervical cancer were calculated,and no significant differences were dentified.Conclusions The revalence of high-risk HPV infection among women attending hospitals for health-examination in Shanghai is similar to the worldwide rate.HPV viral load can distinguish ervical lesions from normal individuals but cannot adequately predict the severity of cervical lesions.

  4. Cervical Cancer

    Centers for Disease Control (CDC) Podcasts

    2007-03-06

    Did you know that cervical cancer rates differ by race/ethnicity and region? Or that cervical cancer can usually be prevented if precancerous cervical lesions are found by a Pap test and treated? Find out how getting regular Pap tests can save a woman's life.  Created: 3/6/2007 by National Breast and Cervical Cancer Early Detection Program.   Date Released: 4/25/2007.

  5. A pooled analysis of continued prophylactic efficacy of quadrivalent human papillomavirus (Types 6/11/16/18) vaccine against high-grade cervical and external genital lesions

    DEFF Research Database (Denmark)

    Kjaer, Susanne K; Sigurdsson, Kristján; Iversen, Ole-Erik;

    2009-01-01

    Quadrivalent human papillomavirus (HPV) vaccine has been shown to provide protection from HPV 6/11/16/18-related cervical, vaginal, and vulvar disease through 3 years. We provide an update on the efficacy of the quadrivalent HPV vaccine against high-grade cervical, vaginal, and vulvar lesions based...... trials (protocols 007, 013, and 015). Vaccine or placebo was given at baseline, month 2, and month 6. Pap testing was conducted at regular intervals. Cervical and anogenital swabs were collected for HPV DNA testing. Examination for the presence of vulvar and vaginal lesions was also done. Endpoints...... included high-grade cervical, vulvar, or vaginal lesions (CIN 2/3, VIN 2/3, or VaIN 2/3). Mean follow-up time was 42 months post dose 1. Vaccine efficacy against HPV 6/11/16/18-related high-grade cervical lesions in the per-protocol and intention-to-treat populations was 98.2% [95% confidence interval (95...

  6. Mapping of HPV transcripts in four human cervical lesions using RNAseq suggests quantitative rearrangements during carcinogenic progression.

    Science.gov (United States)

    Chen, Jinmiao; Xue, Yuezhen; Poidinger, Michael; Lim, Thimothy; Chew, Sung Hock; Pang, Chai Ling; Abastado, Jean-Pierre; Thierry, Françoise

    2014-08-01

    Two classes of Human papillomaviruses (HPV) infect the anogenital track: high risk viruses that are associated with risk of cervical cancer and low risk types that drive development of benign lesions, such as condylomas. In the present study, we established quantitative transcriptional maps of the viral genome in clinical lesions associated with high risk HPV16 or low risk HPV6b. Marked qualitative and quantitative changes in the HPV16 transcriptome were associated with progression from low to high grade lesions. Specific transcripts encoding essential regulatory proteins such as E7, E2, E1^E4 and E5 were identified. We also identified intrinsic differences between the HPV6b-associated condyloma transcript map and that of the HPV16-associated low grade CIN specifically regarding promoter usage. Characterization and quantification of HPV transcripts in patient samples thus establish the impact of viral transcriptional regulation on the status of HPV-associated lesions and may therefore help in defining new biologically-relevant prognosis markers. PMID:25092457

  7. Low NKp30, NKp46 and NKG2D expression and reduced cytotoxic activity on NK cells in cervical cancer and precursor lesions

    Directory of Open Access Journals (Sweden)

    Bravo-Cuellar Alejandro

    2009-06-01

    Full Text Available Abstract Background Persistent high risk HPV infection can lead to cervical cancer, the second most common malignant tumor in women worldwide. NK cells play a crucial role against tumors and virus-infected cells through a fine balance between activating and inhibitory receptors. Expression of triggering receptors NKp30, NKp44, NKp46 and NKG2D on NK cells correlates with cytolytic activity against tumor cells, but these receptors have not been studied in cervical cancer and precursor lesions. The aim of the present work was to study NKp30, NKp46, NKG2D, NKp80 and 2B4 expression in NK cells from patients with cervical cancer and precursor lesions, in the context of HPV infection. Methods NKp30, NKp46, NKG2D, NKp80 and 2B4 expression was analyzed by flow cytometry on NK cells from 59 patients with cervical cancer and squamous intraepithelial lesions. NK cell cytotoxicity was evaluated in a 4 hour CFSE/7-AAD flow cytometry assay. HPV types were identified by PCR assays. Results We report here for the first time that NK cell-activating receptors NKp30 and NKp46 are significantly down-regulated in cervical cancer and high grade squamous intraepithelial lesion (HGSIL patients. NCRs down-regulation correlated with low cytolytic activity, HPV-16 infection and clinical stage. NKG2D was also down-regulated in cervical cancer patients. Conclusion Our results suggest that NKp30, NKp46 and NKG2D down-regulation represent an evasion mechanism associated to low NK cell activity, HPV-16 infection and cervical cancer progression.

  8. MRI Findings of Cervical Spine Lesions among Symptomatic Patients and Their Risk Factors

    Directory of Open Access Journals (Sweden)

    J. Amir orang

    2003-06-01

    Full Text Available Background: Cervical spine and intervertebral discs are potentially prone to functional disorders. Objectives:This study sought type and distribution of different pathologies in the cervical spine and a possible relationship between the MRI findings and the probable risk factors of the degenerative disorders. Material and methods: This descriptive cross-sectional research was carried out from October 2000 to January 2002 in three referral centers in Tehran. All the patients had referred for cervical MRI for neck pain and/or radicular pain. Results: Totally 342 patients entered the study. Sixty percent of patients were male. The mean age was 55.1 12.1 years. Seventy-nine percent of patients had abnormal MRI findings (238 patients (70% had signs of degenerative processes and 31 patients (9% had the other findings with a total 308 pathologies. The most common findings were disc bulging/ protrusion (%21.1, disc dehydration (%20.1, disc herniation (%18.1, and canal stenosis(%17.5. Older age, male gender and history of neck trauma were associated with increasing probability of degenerative changes (P-Values<0.05. Conclusion: Types of cervical spine pathologies are comparable to other reports. The anatomical distribution of disc bulging and protrusion in our study are similar to other reports. Likewise age, gender and a history of trauma to the neck were closely associated with the degenerative signs on the MR images.

  9. Quadrivalent HPV vaccine effectiveness against high‐grade cervical lesions by age at vaccination: A population‐based study

    Science.gov (United States)

    Sundström, Karin; Ploner, Alexander; Uhnoo, Ingrid; Sparén, Pär; Arnheim‐Dahlström, Lisen

    2016-01-01

    Human papillomavirus (HPV) types 16/18, included in HPV vaccines, contribute to the majority of cervical cancer, and a substantial proportion of cervical intraepithelial neoplasia (CIN) grades 2/3 or worse (CIN2+/CIN3+) including adenocarcinoma in situ or worse. The aim of this study was to quantify the effect of quadrivalent HPV (qHPV) vaccination on incidence of CIN2+ and CIN3+. A nationwide cohort of girls and young women resident in Sweden 2006–2013 and aged 13–29 (n = 1,333,691) was followed for vaccination and histologically confirmed high‐grade cervical lesions. Data were collected using the Swedish nationwide healthcare registers. Poisson regression was used to calculate incidence rate ratios (IRRs) and vaccine effectiveness [(1‐IRR)x100%] comparing fully vaccinated with unvaccinated individuals. IRRs were adjusted for attained age and parental education, and stratified on vaccination initiation age. Effectiveness against CIN2+ was 75% (IRR = 0.25, 95%CI = 0.18–0.35) for those initiating vaccination before age 17, and 46% (IRR = 0.54, 95%CI = 0.46–0.64) and 22% (IRR = 0.78, 95%CI = 0.65–0.93) for those initiating vaccination at ages 17–19, and at ages 20–29, respectively. Vaccine effectiveness against CIN3+ was similar to vaccine effectiveness against CIN2+. Results were robust for both women participating to the organized screening program and for women at prescreening ages. We show high effectiveness of qHPV vaccination on CIN2+ and CIN3+ lesions, with greater effectiveness observed in girls younger at vaccination initiation. Continued monitoring of impact of HPV vaccination in the population is needed in order to evaluate both long‐term vaccine effectiveness and to evaluate whether the vaccination program achieves anticipated effects in prevention of invasive cervical cancer. PMID:26856527

  10. Cervical Cytology Smear Combined Colposcopy in the Diagnosis of Cervical Lesions in Clinical Observa-tion%宫颈细胞学涂片联合阴道镜诊断宫颈病变的临床观察

    Institute of Scientific and Technical Information of China (English)

    许盛芳

    2015-01-01

    Objective To explore the cervical cytological smear the clinical effect of combined colposcopy in the di-agnosis of cervical lesions.Methods Between January 2013 and December 2013 in our hospital to accept the cervical cytological smear combined colposcope examination of 880 cases of married women as the research object, on the cervical lesions of inspection, analysis of the clinical condition.Results Of cervical smear cytologic examination and vaginoscope diagnosis coincidence rate in RCI score photogenic compared with significant difference ( P<0.05) , after statistics treat-ment, under colposcopy in the diagnosis of CIN sensitivity was 94.84%, specificity is 88.81%, both the positive pre-dictive value was 72.31% and negative predictive value was 95.63%.Conclusions Cervical cytological smear com-bined colposcope examination before screening of cervical lesions can ensure cerical cancer lesions and early diagnosis of cervical cancer, can also become local screening test for cervical cancer lesion before effective policies.%目的:探讨宫颈细胞学涂片联合阴道镜诊断宫颈病变的临床效果。方法选取2013年1月~2013年12月在我院接受宫颈细胞学涂片联合阴道镜检查的880例已婚妇女作为研究对象,对其进行宫颈病变的检查,分析其临床情况。结果宫颈涂片细胞学检查与阴道镜下RCI评分诊断在符合率上相比较差异具有显著性( P<0.05),经统计学处理后,阴道镜下诊断CIN的敏感性是94.84%,特异性是88.81%,两者联合的阳性预测值是72.31%,阴性预测值是95.63%。结论宫颈细胞学涂片联合阴道镜检查筛选子宫颈病变可以保证宫颈癌前病变和前期宫颈癌的确诊,还可以成为当地筛选检查宫颈癌前病变的有效政策。

  11. Restoration of non-carious cervical lesions with ceramic inlays: A possible model for clinical testing of adhesive cements

    Directory of Open Access Journals (Sweden)

    Michal Staninec

    2012-01-01

    Full Text Available Introduction: There are many luting cements coming to market which claim to be adhesive, but there is no clinical protocol currently for testing these claims. There is a standardized protocol for testing direct restorations bonded to dentin and it is used extensively. Case Report: We describe a clinical procedure for restoring a non-carious cervical lesion (NCCL with a ceramic inlay using Computer-Aided Design and Computer-Aided Manufacturing (CAD-CAM technology and an adhesive resin cement.The procedure was straightforward and the result was good at one month. Discussion: NCCL′s can be restored with CAD-CAM technology in one appointment. This technique can be used to clinically test adhesion of luting cements to dentin, similarly to the current standard for direct restorations.

  12. ANÁLISIS COMPUTACIONAL DE LESIONES CERVICALES NO CARIOSAS EN UN PREMOLAR SUPERIOR COMPUTER ANALYSIS OF NON-CAVITY CERVICAL LESIONS IN AN UPPER PREMOLAR

    Directory of Open Access Journals (Sweden)

    Patricio Cendoya

    2007-08-01

    Full Text Available Se presenta un modelo tridimensional de elementos finitos para investigar el efecto producido por las fuerzas de oclusión en la distribución y magnitud del campo tensional sobre un premolar superior. De esta forma, se busca definir cuál es la situación de carga de oclusión más crítica desde el punto de vista tensional sobre el esmalte dental en la zona cervical que pudiese dar origen a una lesión del tipo no cariosa. Utilizando elementos finitos hexaédricos lineales y procesando el modelo con el programa SAMCEF se realiza un análisis numérico estático lineal considerando que el premolar es isótropo y homogéneo. Se definen siete estados de carga asociados a una fuerza de oclusión de 170 N para las cuales se investiga la distribución y magnitud de los campos tensionales sobre el premolar. Los resultados numéricos permiten concluir que las tensiones máximas sobre el premolar tienden a concentrarse en la zona cervical alcanzando sus valores máximos cuando la carga de 170 N actúa de forma inclinada a 45º con respecto a la vertical. La relevancia de la presente investigación radica en el hecho que numéricamente se demuestra que la distribución y zona en donde los campos tensionales tienden a concentrase y alcanzar sus valores máximos son coincidentes con las zonas del diente en donde clínicamente se observan lesiones no cariosas.A three-dimensional model of finite elements in order to investigate the effect produced by occlusion forces on both distribution and magnitude of the tensional field on an upper premolar is hereby presented. Thus, defining from a tensional point of view, which is the situation of the most critical occlusion loading on dental enamel capable to produce a non-cavity lesion is intended. By using finite linear hexahedral elements and processing the models through SAMCEF software, a static linear numerical analysis is performed, considering that premolar is isotropic and homogeneous. Seven load states

  13. The significance of HPV genotyping in cervical lesions%宫颈组织病变与检测HPV基因分型的意义

    Institute of Scientific and Technical Information of China (English)

    张达衡; 陈红玲; 杨春媚; 陈瑞林

    2013-01-01

    目的 了解宫颈病变患者人乳头瘤病毒HPV感染率及基因型分布情况.探讨人乳头瘤病毒基因分型与宫颈组织病变的相关性,为临床宫颈癌的筛查、防治提供参考.方法 收集在本院诊治2 000例疑似宫颈病变女性,应用基因芯片技术检测23种HPV基因型,统计HPV各基因型的感染率,分析HPV及基因型分布与子宫病变的关系.结果 2000例宫颈疑似宫颈病变女性中,基因芯片检测出的HPV阳性标本662例,HPV感染率为33.1%(662/2 000).354例宫颈不同程度病变组织中HPV阳性295例,其中LSIL,HSIL及宫颈癌样本中HPV的检出率分别为76.6%(157/205)、91.9%(125/136)、100%(13/13).共检出12种HPV基因型,检出率最高的5种基因型分别为HPV16,18,58,31,52.宫颈病变级别越高,HPV多重感染率越高.结论 HPV亚型感染与宫颈组织不同程度病变密切相关,HPV基因分型检测及诊断在宫颈病变及宫颈癌筛查及防治过程中具有重要意义.%Objective To investigate the infection rate of human papillomavirus (HPV) and distribution of HPV genotypes in cervical lesions,and discuss the correlationbetween HPV genotypes and cervical lesions to provide a reference for cervical cancer screening,prevention and treatment.Methods 2 000 cases of women with suspected cervical lesions were detected by 23 cases of HPV genotyping based on gene chip technology,and infection rates of HPV genotypes and the correlation between HPV genotypes and cervical lesions were analyzed.Results 662 cases were HPV positive in 2 000 cases of women with suspected cervical lesions,with the HPV infection rate of 33.1 % (662/2 000),and 295 cases were HPV positive in 354 cases of women with cervical lesions.For LSIL,HSIL and cervical cancer,HPV detection rates were 76.6% (157/205),91.9% (125/136),100% (13 / 13),respectively.12 kinds of HPV genotypes were detected,and the top five genotypes of the highest detection rate were HPV16,18,58,31,52.The higher

  14. Screening Methods for Cervical Lesion and its New Development%宫颈病变筛查方法及其新进展综述

    Institute of Scientific and Technical Information of China (English)

    邓晓红

    2016-01-01

    综述宫颈病变的筛查技术及新进展,早期发现宫颈癌和提高治愈率。宫颈癌是最常见的妇科恶性肿瘤之一,严重威胁着妇女的健康,且近几年趋向年轻化。人乳头瘤病毒(HPV DNA)感染是引起宫颈病变尤其是宫颈癌的主要原因,TCT是宫颈细胞学筛查的一种新的全自动细胞学检测技术,阴道镜是宫颈早期浸润癌筛查的重要应用。三者联合筛查宫颈病变,早期发现、早期诊断和早期干预宫颈的癌前病变,能够有效预防宫颈癌发生,降低宫颈癌的发病率和死亡率。%This paper reviews screening technologies and new developments for cervical lesions, early detection of cervical cancer, so as to improve in cure rate. Cervical cancer is one of the most common gynecologic malignant tumor, it is a serious threat to women's health, and in recent years it trends to target the younger ages. Human papilloma virus (HPV DNA) infection is the primary cause of cervical lesions, especially for cervical cancer. TCT is a new automatic cytology detection technology for cervical cytology screening. Colposcopy is an important application in early invasive cervical cancer screening. Combining the above mentioned three screening technologies could achieve the purposes of early detection, early diagnosis and early intervention of cervical precancerous lesions, so as to effectively prevent the occurrence of cervical cancer, reduce the morbidity and mortality of cervical cancer.

  15. Metabonomic signature analysis of cervical carcinoma and precancerous lesions in women by (1)H NMR spectroscopy.

    Science.gov (United States)

    Hasim, Ayshamgul; Ali, Mayinuer; Mamtimin, Batur; Ma, Jun-Qi; Li, Qiao-Zhi; Abudula, Abulizi

    2012-06-01

    (1)H nuclear magnetic resonance (NMR)-based metabonomics has been used to characterize the metabolic profiles of cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CSCC). Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used to model the systematic variation related to patients with CIN or CSCC with healthy controls. Potential metabolic biomarkers were identified using database comparisons, and the one-way analysis of variance (ANOVA) test was used to examine the significance of the metabolites. Compared with plasma obtained from the healthy controls, plasma from patients with CIN had higher levels of very-low density lipoprotein (VLDL), acetone, unsaturated lipid and carnitine, together with lower levels of creatine, lactate, isoleucine, leucine, valine, alanine, glutamine, histidine, glycine, acetylcysteine, myo-inositol, choline and glycoprotein. Plasma from patients with CSCC had higher levels of acetate and formate, together with lower levels of creatine, lactate, isoleucine, leucine, valine, alanine, glutamine, histidine and tyrosine compared with the plasma of the healthy controls. In addition, compared with the plasma of patients with CIN, the plasma of CSCC patients had higher levels of acetate, formate, lactate, isoleucine, leucine, valine, alanine, glutamine, histidine, tyrosine, acetylcysteine, myo-inositol, glycoprotein, α-glucose and β-glucose, together with lower levels of acetone, unsaturated lipid and carnitine. Moreover, the profiles showed high feasibility and specificity by statistical analysis with OPLS-DA compared to the Thinprep cytology test (TCT) by setting the histopathological outcome as standard. The metabolic profile obtained for cervical cancer is significant, even for the precancerous disease. This suggests a systemic metabolic response to cancer, which may be used to identify potential early diagnostic biomarkers of the cancer and to establish

  16. Effect Observation on HPV, TCT and Vaginal Examination in Cervical Le-sions and Cervical Screening%宫颈病变和宫颈筛查应用HPV、TCT和阴道镜检查的效果探讨

    Institute of Scientific and Technical Information of China (English)

    金伟蓉; 孔彩霞

    2015-01-01

    目的:探讨宫颈病变和宫颈筛查应用HPV、TCT和阴道镜检查的效果。方法整群选取2013年5月—2015年5月于该院接受TCT及HPV检查的妇女1280例。对这些患者再进行阴道镜检查以及宫颈组织活检,然后将检验的结果与病理结果进行统计学的分析比对。结果 TCT检查的阳性检出率为9.61%(123/1280);HPV检查的阳性检出率为12.03%(154/1280)。结论临床上对宫颈病变结合筛查可联合CTC、HPV以及阴道镜检查,这样可提高宫颈病变的检出率,减少漏诊,对临床的及时治疗提供极大的帮助。%Objective To observe the effect of HPV, TCT and vaginal examination in cervical lesions and cervical screen-ing. Methods 1280 cases of women who received TCT and HPV examination in our hospital from May 2013 to May 2015 were selected and were given vaginal examination and cervical biopsy, the test results and the pathological results were compared and analyzed statistically. Results The positive detection rate of TCT was 9.61% (123/1280)and 12.03%(154/1280)of HPV. Conclusion HPV, TCT and vaginal examination in treatment of cervical lesions and cervical screening can improve the detection rate of cervical lesions, reduce missed diagnosis and offer great help to timely treatment clinically.

  17. Trends in the incidence of cervical cancer and severe precancerous lesions in Denmark, 1997-2012

    DEFF Research Database (Denmark)

    Baldur-Felskov, Birgitte; Munk, Christian; Nielsen, Thor Schütt Svane;

    2015-01-01

    papillomavirus (HPV) vaccination. METHODS: Using nationwide registries, we estimated age-specific and age-standardized incidence rates and estimated annual percentage change (EAPC). RESULTS: The incidence of SCC decreased significantly, especially in women aged ≥45 years [EAPC: -3.1 % (95 % CI -4.3 to -2...... or the incidence of adenocarcinoma, which is increasing. Decreases in the incidences of CIN3 and AIS in age groups with high HPV vaccine coverage may herald a future decrease in cervical cancer incidence in young Danish women....

  18. Sustainability of abrasive processes

    DEFF Research Database (Denmark)

    Aurich, J.C.; Linke, B.; Hauschild, Michael Zwicky;

    2013-01-01

    This paper presents an overview of research on sustainability of abrasive processes. It incorporates results from a round robin study on ‘‘energy-efficiency of abrasive processes’’ which has been carried out within the scientific technical committee ‘‘abrasive processes’’ (STC G) of CIRP...

  19. Risk assessment on liquid based cytology test and human papilloma virus L1 Nuclecapsid protein detection in the diagnosis of cervical lesions and its progress

    Institute of Scientific and Technical Information of China (English)

    Ling-Zhi Chen

    2016-01-01

    Objective: To discuss the clinical value of liquid based cytology test in the diagnosis of cervical lesions and to evaluate the expression difference and significance of human papilloma virus L1(HPV L1) protein in exfoliative cells of different cervical lesions. Methods: A total of 1 400 cases of patients in gynaecological clinics of our hospital from January 2009 to August 2013 were selected and were given liquid based cytology test(LCT) examination and HPV L1 protein expression detection in exfoliative cells. Meanwhile, cervical biopsy pathology results were taken as baseline for make a comparison. Results: (1) In such1400 cases of women: LCT(+)-30 cases, accounting for 2.1%, HPV L1 protein detection(+)-18 cases, accounting for 1.29%; LCT and HPV L1 protein detection(+)-8 cases, LCT and HPV L1 protein detection(-)- 1360 cases. (2)Pathological tissue biopsy was performed on 30 cases of patients with LCT(+), results showed that(+)were 23 cases; pathological tissue biopsy was performed on 18 cases of patients with HPV L1 protein(+),results showed that (+)were 14 cases. Among 1 360 cases with LCT and HPV L1 protein detection(-), 497 cases were willing to accept the pathological biopsy, and the results showed that 1 case presented(+), accounting for 0.2%. Total 545 cases were willing to accept the pathological biopsy, among whom, 38 cases presented(+), accounting for 6.97%;(3)Sensitivity and specificity of LCT combined with HPV L1 protein detection were higher than single-method detection, and difference had statistical significance. Conclusion: liquid based cytology test combined with HPV L1 protein detection could improve the diagnostic rate of cervical lesions, and were expected as the effective method in the early clinical screening of cervical neoplasia lesions.

  20. Prevalence of human papillomavirus in archival samples obtained from patients with cervical pre-malignant and malignant lesions from Northeast Brazil

    Directory of Open Access Journals (Sweden)

    Prado José CM

    2010-04-01

    Full Text Available Abstract Background Human Papillomavirus (HPV is considered as a necessary, but not sufficient, cause of cervical cancer. In this study, we aimed to assess the prevalence of HPV in a series of pre-malignant and malignant cervical lesion cases, to identify the virus genotypes, and to assess their distribution pattern according to lesion type, age range, and other considered variables. The samples were submitted to histopathological revision examination and analysed by polymerase chain reaction (PCR for the presence of HPV DNA, followed by HPV typing by dot blot hybridisation. Findings Of the analysed samples, 53.7% showed pre-malignant cervical lesions, and 46.3% presented with cervical cancer. Most cancer samples (84.1% were classified as invasive carcinoma. The mean age of these cancer patients was 47.3 years. The overall HPV prevalence was 82.4% in patients with pre-malignant lesions and 92.0% in the cancer patients. HPV 16 was the most prevalent type, followed by HPV 18 and 58, including both single and double infections. Double infection was detected in 11.6% of the samples, and the most common combination was HPV 16+18. Conclusions Cervical cancer appears to occur in women in a lower age range in the studied area, compared to the situation in other Brazilian regions. Furthermore, among the patients with CIN 3 and those with cancer, we observed a higher proportion of married women, women with more than one sexual partner, smokers, and individuals with less than an elementary education, relative to their counterparts. Findings The overall HPV prevalence was 82.4% in patients with pre-malignant lesions and 92.0% in the cervical cancer patients from Northeast Brazil. HPV 16 was the most prevalent type, followed by HPV 18 and 58. The most common double infection was HPV 16+18. Cervical cancer appears to occur in women in a lower age range in the Northeast Brazil. Among the patients with CIN 3 and those with cancer, we observed a higher

  1. [Expression of TREM-1 in patients with invasive cervical cancer and precursor lesions].

    Science.gov (United States)

    Anaya-Prado, Roberto; Norzgaray-Ibarra, Fabiola Geovanna; Bravo-Cuéllar, Alejandro; Pérez-Avila, Carlos Eduardo; Schadegg-Peña, Daniel; Anaya-Fernández, Michelle M

    2015-01-01

    Introducción: se ha demostrado que la glicoproteína TREM-1 pertenece a la superfamilia de las inmunoglobulinas que induce la secreción de varias citocinas proinflamatorias. El objetivo de este trabajo fue medir la expresión de TREM-1 en pacientes con cáncer cervical. Métodos: en este estudio transversal analítico incluimos 4 grupos de pacientes: GI: mujeres con lesión intraepitelial (LI) escamosa de bajo grado (n = 15 p/g); GII: pacientes con LI escamosa de alto grado (n = 9 p/g); GIII: pacientes con cáncer cervical invasor (n = 9 p/g), y GIV: pacientes sanas (n = 15 p/g). En todas las pacientes se midió la expresión de TREM-1 y el Índice Medio de Fluorescencia (IMF) en neutrófilos y monocitos, así como los niveles de leucocitos, neutrófilos y monocitos. Usamos t de Student para muestras independientes. Para estas mismas variables, aplicamos prueba de suma de rangos de Mann-Whitney, ANOVA y Turkey. Para las variables cualitativas se utilizó la prueba de Chi cuadrada. Resultados: los porcentaje de expresión de TREM-1 en neutrófilos y monocitos, además del IMF en neutrófilos en los 4 grupos, no fue significativamente diferente. El IMF de TREM-1 en monocitos fue significativamente diferente al comparar el grupo II y grupo III frente al grupo IV (p estudio documenta una mayor expresión de TREM-1 en monocitos de pacientes con cáncer avanzado.

  2. Detection, management, and follow-up of pre-malignant cervical lesions and the role for human papillomavirus.

    NARCIS (Netherlands)

    Hamont, D. van; Bekkers, R.L.M.; Massuger, L.F.A.G.; Melchers, W.J.G.

    2008-01-01

    Cervical cytological pathology is common. Prevention of cervical cancer by detecting the disease process at an early and pre-malignant stage is practised globally either through population-based screening programmes (PSP) or through non-organised ones. High-grade cervical intraepithelial neoplasia (

  3. Cytohistological correlation and accuracy of the pap smear test in diagnosis of cervical lesions: a hospital based cross-sectional study from Odisha, India

    Directory of Open Access Journals (Sweden)

    Reena Naik

    2015-09-01

    Full Text Available Background Cervical carcinoma is one of the most common causes of mortality among women.Early detection can be done by Papanicolaou (Papsmear test - a simplest, safe, cost effective and non invasive procedure. The main objective of this study was to categorize Pap smears for cytohistologicalexamination as well as clinically correlate to analyze sensitivity, specificity and positive predictive value of Pap smear. Methods In this prospective study 417 Pap smears performed, cytohistological correlation was done in 104 cases because patients had undergone both Pap smear and cervical biopsy.Detailed history was taken and clinical examination was done. Pap smear sample was collected from cervix and reporting was made according to Bethesda 2001 classification.Cytological findings were correlated with histopathology. Results Overall concordance rate was 60.7%. Concordance rate for malignancy was 100%, for inflammatory lesions 70.8% and for cervical intraepithelial neoplasia (CIN 33.3%. Common age group presented for screening was40-50 years.Commonest clinical presentationwas bleeding per vaginum. Conclusion The study provides good cyto-histopathology correlation in detecting high grade lesions and malignancy. Although Pap smear sensitivity was low but can be increased by adequate sampling and avoiding technical errors.Bethesda system is strongly recommended for adequacy of sampling to minimize inconsistency.Regular screening should be advised to the patients for the early detection of cervical carcinoma.

  4. Resolución endovascular de lesiones por arma de fuego en la región cervical

    Directory of Open Access Journals (Sweden)

    Sebastián Rizzone

    2016-01-01

    Full Text Available Introducción: Las heridas producidas por armas de fuego que comprometen la región cervical son materia de controversia en cuanto a cuál es el mejor método para tratarlas. La resolución endovascular de este tipo de patología ha cobrado gran relevancia en los últimos años debido al desarrollo de nuevos dispositivos y, consecuentemente, a la menor incidencia de complicaciones inherentes al procedimiento. Objetivo: Determinar el rol del tratamiento endovascular en la resolución de las lesiones cervicales por arma de fuego. Material y métodos: Se incluyeron 76 pacientes que sufrieron lesiones cervicales por arma de fuego, a los cuales se les realizó angiografía de los vasos supraaórticos para decidir la conducta a seguir. Resultados: Del total de 76 pacientes, 47 (61,8% presentaron una angiografía patológica; de estos, en 26 se decidió la resolución endovascular de la lesión, implantándose 1,1 stents por paciente. La estadía intrahospitalaria fue en promedio de 10,4 días. A los 30 días de seguimiento, 2 pacientes presentaron secuela neurológica y uno evolucionó con sepsis. En el seguimiento alejado con un promedio de 4 años se registró una muerte a los 117 días de realizado el procedimiento. Conclusiones: La intervención endovascular ha ganado mayor protagonismo con el desarrollo de nuevos dispositivos que permiten el tratamiento de patologías cada vez más complejas y reducir el tiempo de internación intrahospitalaria, como también las complicaciones relacionadas con el procedimiento.

  5. Bulky Early-Stage Cervical Cancer (2-4 cm Lesions): Upfront Radical Trachelectomy or Neoadjuvant Chemotherapy Followed by Fertility-Preserving Surgery: Which Is the Best Option?

    Science.gov (United States)

    Plante, Marie

    2015-05-01

    Radical trachelectomy is now recognized as a valid treatment option for young women with early-stage cervical cancer with lesions measuring less than 2 cm. However, for women with bulky lesions measuring greater than 2 cm, few data are available in the literature to guide management. There are currently 2 options available: either upfront radical trachelectomy or neoadjuvant chemotherapy followed by fertility-preserving surgery. Overall, both options offer very good oncologic outcome; however, the rate of fertility preservation and obstetrical outcome seem superior after neoadjuvant chemotherapy. Advantages and disadvantages of both options are discussed and a thorough literature review is provided. Issues to be further studied are also outlined.

  6. Prevalence and risk factors of non-carious cervical lesions related to occupational exposure to acid mists.

    Science.gov (United States)

    Bomfim, Rafael Aiello; Crosato, Edgard; Mazzilli, Luiz Eugênio Nigro; Frias, Antonio Carlos

    2015-01-01

    This study evaluates the prevalence and risk factors of non-carious cervical lesions (NCCLs) in a Brazilian population of workers exposed and non-exposed to acid mists and chemical products. One hundred workers (46 exposed and 54 non-exposed) were evaluated in a Centro de Referência em Saúde do Trabalhador - CEREST (Worker's Health Reference Center). The workers responded to questionnaires regarding their personal information and about alcohol consumption and tobacco use. A clinical examination was conducted to evaluate the presence of NCCLs, according to WHO parameters. Statistical analyses were performed by unconditional logistic regression and multiple linear regression, with the critical level of p smoking (p = 0.01) and age group (p = 0.04). The prevalence of NCCLs in the study population was particularly high (76.84%), and the risk factors for NCCLs were age, exposure to acid mists and smoking habit. Controlling risk factors through preventive and educative measures, allied to the use of personal protective equipment to prevent the occupational exposure to acid mists, may contribute to minimizing the prevalence of NCCLs.

  7. Co-infections associated with human immunodeficiency virus type 1 in pregnant women from southern Brazil: high rate of intraepithelial cervical lesions

    Directory of Open Access Journals (Sweden)

    Michele Tornatore

    2012-03-01

    Full Text Available Human immunodeficiency virus type 1 (HIV-positive pregnant women require specific prophylactic and therapeutic approaches. The efficacy of established approaches is further challenged by co-infection with other sexually transmitted diseases (STDs. The objective of this study was to determine the prevalence of co-infections in pregnant women infected with different HIV-1 subtypes and to relate these findings, together with additional demographic and clinical parameters, to maternal and infant outcomes. Blood samples from pregnant women were collected and tested for syphilis, hepatitis B virus (HBV and hepatitis C virus (HCV. Human papillomavirus (HPV diagnosis was evaluated by the presence of alterations in the cervical epithelium detected through a cytopathological exam. Medical charts provided patient data for the mothers and children. Statistical analyses were conducted with STATA 9.0. We found a prevalence of 10.8% for HCV, 2.3% for chronic HBV, 3.1% for syphilis and 40.8% for HPV. Of those co-infected with HPV, 52.9% presented high-grade intraepithelial lesions or in situ carcinoma. Prematurity, birth weight, Apgar 1' and 5' and Capurro scores were similar between co-infected and non-co-infected women. The presence of other STDs did not impact maternal and concept outcomes. More than half of the patients presenting cervical cytology abnormalities suggestive of HPV had high-grade squamous intraepithelial lesions or cervical cancer, evidencing an alarming rate of these lesions.

  8. Risk factors for cervical carotid and intracranial cerebrovascular lesions in patients undergoing coronary artery bypass grafting. Preoperative evaluation using magnetic resonance imaging and angiography

    Energy Technology Data Exchange (ETDEWEB)

    Makino, Masahiro [Kyoto Prefectural Univ. of Medicine (Japan). Research Inst. for Neurological Diseases and Geriatrics

    2001-12-01

    Recently neurologic complications after coronary artery bypass grafting (CABG) have received increasing attention. There is no detailed report about the risk factors for these complications, although stenosis in the cervical and intracranial arteries, especially in Japanese patients, latent ischemic brain lesions and preoperative neurological conditions are related to these events. In this prospective study, we evaluated occlusive lesions in the cervical carotid and intracranial arteries, silent brain infarction and cerebral deep white matter lesion with MRA and MRI in patients scheduled to undergo CABG to determine the prevalence of occlusive diseases in cervical carotid and intracranial arteries, latent ischemic change in the brain in this population and to identify preoperative risk factors for these patients. The subjects were 144 consecutive patients (103 men and 41 women, mean age 65.9{+-}9.2 years old) who were scheduled for CABG under elective conditions and who were examined by the same MRI apparatus using the same protocol between November 1998 and March 2001. After routine neurological examination and Mini-Mental State Examination (MMSE) were completed, MRI and MRA were obtained, then the prevalence of abnormalities on MRI and MRA studies and risk factors were evaluated. Cervical carotid artery stenosis with {>=}50% luminal narrowing was detected in 29.2% of the subjects, and that with {>=}75% luminal narrowing was detected in 16.0% of the subjects. Intracranial arterial stenosis showing {>=}50% luminal narrowing was detected in 38.2% of subjects, and that showing {>=}75% luminal narrowing was detected in 19.4% of subjects. Brain infarction was observed in 74.3% of subjects, cerebral deep white matter lesion showing grade 2 or higher on Fazekas classification was observed in 17.4% of the subjects. The characteristics, including possible risk factors of subjects with and without these abnormal findings, were compared. Patients with cervical carotid

  9. Risk factors for cervical carotid and intracranial cerebrovascular lesions in patients undergoing coronary artery bypass grafting. Preoperative evaluation using magnetic resonance imaging and angiography

    International Nuclear Information System (INIS)

    Recently neurologic complications after coronary artery bypass grafting (CABG) have received increasing attention. There is no detailed report about the risk factors for these complications, although stenosis in the cervical and intracranial arteries, especially in Japanese patients, latent ischemic brain lesions and preoperative neurological conditions are related to these events. In this prospective study, we evaluated occlusive lesions in the cervical carotid and intracranial arteries, silent brain infarction and cerebral deep white matter lesion with MRA and MRI in patients scheduled to undergo CABG to determine the prevalence of occlusive diseases in cervical carotid and intracranial arteries, latent ischemic change in the brain in this population and to identify preoperative risk factors for these patients. The subjects were 144 consecutive patients (103 men and 41 women, mean age 65.9±9.2 years old) who were scheduled for CABG under elective conditions and who were examined by the same MRI apparatus using the same protocol between November 1998 and March 2001. After routine neurological examination and Mini-Mental State Examination (MMSE) were completed, MRI and MRA were obtained, then the prevalence of abnormalities on MRI and MRA studies and risk factors were evaluated. Cervical carotid artery stenosis with ≥50% luminal narrowing was detected in 29.2% of the subjects, and that with ≥75% luminal narrowing was detected in 16.0% of the subjects. Intracranial arterial stenosis showing ≥50% luminal narrowing was detected in 38.2% of subjects, and that showing ≥75% luminal narrowing was detected in 19.4% of subjects. Brain infarction was observed in 74.3% of subjects, cerebral deep white matter lesion showing grade 2 or higher on Fazekas classification was observed in 17.4% of the subjects. The characteristics, including possible risk factors of subjects with and without these abnormal findings, were compared. Patients with cervical carotid lesions were

  10. Anti-Nogo-A antibody treatment does not prevent cell body shrinkage in the motor cortex in adult monkeys subjected to unilateral cervical cord lesion

    Directory of Open Access Journals (Sweden)

    Mir Anis

    2008-01-01

    Full Text Available Abstract Background After unilateral cervical cord lesion at the C7/C8 border interrupting the dorsolateral funiculus in adult monkeys, neutralization of Nogo-A using a specific monoclonal antibody promoted sprouting of corticospinal (CS axons rostral and caudal to the lesion and, in parallel, improved functional recovery. In monkeys lesioned but not treated with the anti-Nogo-A antibody, the CS neurons in the contralesional primary motor cortex (M1 survived to the axotomy, but their soma shrank. Because the anti-Nogo-A treatment induces regeneration and/or sprouting of CS axons, it may improve access to neurotrophic factors. The question therefore arises as to whether anti-Nogo-A treatment prevents the soma shrinkage observed in the contralesional M1? Results Using the marker SMI-32, a quantitative and qualitative anatomical assessment of the pyramidal neurons in the layer V (thus including the CS cells in M1 was performed and compared across three groups of animals: intact monkeys (n = 5; monkeys subjected to the cervical cord lesion and treated with a control antibody (n = 4; monkeys with the cervical lesion and treated with anti-Nogo-A antibody (n = 5. SMI-32 positive neurons on the side contralateral to the lesion were generally less well stained than those on the ipsilesional hemisphere, suggesting that they expressed less neurofilaments. Nevertheless, in all three groups of monkeys, the amount of SMI-32 positive neurons in both hemispheres was generally comparable, confirming the notion that most axotomized CS neurons survived. However, shrinkage of CS cell body area was observed in the contralesional hemisphere in the two groups of lesioned monkeys. The cell surface shrinkage was found to be of the same magnitude in the monkeys treated with the anti-Nogo-A antibody as in the control antibody treated monkeys. Conclusion The anti-Nogo-A antibody treatment did not preserve the axotomized CS cells from soma shrinkage, indicating that the

  11. P16INK4a: a potential diagnostic adjunct for prediction of high-grade cervical lesions in liquid-based cytology: with HPV testing and histological correlation.

    Science.gov (United States)

    Wong, Y P; Abdul Raub, S H; Mohd Dali, A Z H; Kassim, F; Visvalingam, V; Zakaria, Z; Kamaluddin, M A; Noor Akmal, S

    2016-08-01

    Human papillomavirus (HPV) is a necessary cause of cervical cancer and its precursors. Increased expression of high-risk hrHPV viral oncogenes in abnormal cells might increase the expression of p16INK4a. We aimed to determine the role of p16INK4a in detecting hrHPV-transformed epithelial cells in liquid-based cervical cytology, and compared the results with hrHPV DNA testing by realtime polymerase chain reaction (RT-PCR). Fifty-seven cytological samples were tested for p16INK4a immunomarker and hrHPV DNA. Test performance of both tests was determined by comparing sensitivity, specificity and predictive values using available histological follow-up data as gold standard. Of 57 samples, 36 (63.2%) showed immunoreactivity for p16INK4a and 43 (75.4%) were hrHPV-infected. A fairly low concordance rate (k = 0.504) between p16INK4a immunolabelling and hrHPV DNA status was noted. For prediction of cervical intraepithelial neoplasia (CIN) II and worse lesions, p16INK4a had a sensitivity and specificity of 93.5% and 60%; whereas hrHPV DNA testing had a sensitivity and specificity of 100% and 20%. Dual testing by combining p16INK4a and hrHPV showed sensitivity and specificity of 100% and 33.3%. In conclusion, p16INK4a is useful in predicting severity of the cytological abnormalities. Although p16INK4a is more specific but less sensitive than hrHPV in detecting high-grade cervical lesions, a combination of both tests failed to demonstrate significant improvement in diagnostic sensitivity, specificity and predictive value. Larger-scale prospective studies are required to assess further whether this biomarker should be routinely used as primary screening tool independently or in combination with hrHPV testing to improve diagnostic accuracy in cervical cytology. PMID:27568665

  12. RARβ在宫颈癌及癌前病变中的表达调控和意义%The association of altered RARβ gene expression with the cervical lesion pathogenesis

    Institute of Scientific and Technical Information of China (English)

    Nafeisha Kadeer; Hui He; Abida Abudoukadeer; Anaguli Ababaikeli; Abulizi Abudula

    2011-01-01

    Objective:Previous reports have shown that the gene promoter region of retinoic acid receptor β (RARβ) was hypermethylated in cervical carcinoma,implying the inhibition of gene transcription.The aim of this study was to investigate the association of cervical cancer development with the RARβ gene expression at the mRNA and protein level to assess the impact of RARβ as a marker for early detection of the cancer.Methods:We collected 126 cases of formalin fixed and paraffin embedded cervical tissue specimens as well as 37 cases of fresh tissue samples from women with cervicitis,cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma (CSCC).The RARβ mRNA and protein expression was detected by quantitative RT-PCR and immunohistochemistry,respectively.Results:(1) The mRNA expression of RARβ in CIN and cervical cancer was markedly decreased compared to cervicitis with a statistically very significant difference,but no difference was found between CIN and cervical cancer.(2) RARβ protein was normally expressed in the epithelial cells of cervicitis and partially lost in a few cases,but with the development of cervical lesion pathogenesis and cancer,a significant loss of protein expression was detected in CIN (38%) and CSCC (57%) compared to cervicitis (P < 0.01).Conclusion:The downregulation of RARβ transcription or loss of protein expression is an important indicator of cervical cancer and its precursur lesions.The detection of RARβ expression coupled with aberrant methylation of the gene may become a biomarker for the early prognosis or diagnosis of the cancer.

  13. AN UPWARD TREND IN DNA P16INK4A METHYLATION PATTERN AND HIGH RISK HPV INFECTION ACCORDING TO THE SEVERITY OF THE CERVICAL LESION

    Directory of Open Access Journals (Sweden)

    Fernanda Nahoum Carestiato

    2013-09-01

    Full Text Available SUMMARY High-risk human papillomavirus (hr-HPV infection is necessary but not sufficient for cervical cancer development. Recently, P16INK4A gene silencing through hypermethylation has been proposed as an important cofactor in cervical carcinogenesis due to its tumor suppressor function. We aimed to investigate P16INK4A methylation status in normal and neoplastic epithelia and evaluate an association with HPV infection and genotype. This cross-sectional study was performed with 141 cervical samples from patients attending Hospital Moncorvo Filho, Rio de Janeiro. HPV detection and genotyping were performed through PCR and P16INK4A methylation by nested-methylation specific PCR (MSP. HPV frequency was 62.4% (88/141. The most common HPV were HPV16 (37%, HPV18 (16.3% and HPV33/45(15.2%. An upward trend was observed concerning P16INK4A methylation and lesion degree: normal epithelia (10.7%, low grade lesions (22.9%, high grade (57.1% and carcinoma (93.1% (p < 0.0001. A multivariate analysis was performed to evaluate an association between methylation, age, tobacco exposure, HPV infection and genotyping. A correlation was found concerning methylation with HPV infection (p < 0.0001, hr-HPV (p = 0.01, HSIL (p < 0.0007 and malignant lesions (p < 0.0001. Since viral infection and epigenetic alterations are related to cervical carcinoma, we suggest that P16INK4A methylation profile maybe thoroughly investigated as a biomarker to identify patients at risk of cancer.

  14. SPECTRUM OF CERVICAL LESIONS IN CIMS, BILASPUR: A 5 YEAR RETROSPECTIVE STUDY OF 215 CASES IN A TERTIARY HOSPITAL OF CENTRAL INDIA

    Directory of Open Access Journals (Sweden)

    Sadhna

    2015-10-01

    Full Text Available BACKGROUND AND OBJEC TIVE: Cancer of the cervix is a leading cause of morbidity and mortality among women W orld W ide. Therefore to curb the disease there is need of awareness of this disease. This study is aimed to categorize the premalignant and malignant lesions at the earliest, to reduce the mortality and morbidity. It also aimed to know the incidence of malignancies at our set up and to calculate distribution of patient in relation to parity and symptoms. DESIGN AND SETTING: Study included 215 patients with complain of white dis charge per vagina, per vaginal bleeding and backache attending the gynecology OPD over a period of 05 years. PATIENT AND METHODS: Patients were subjected to cervical biopsy and biopsies were sent to pathology department. Detailed clinical history, age, age at marriage, parity and socio - economic status was obtained. RESULT: Result were classified histopathological l y as inflammatory lesions 70 cases, polypoidal lesion were 30, LSIL accounted for 32 cases and HSIL were 20 cases and 30 were malignant lesions. U nder malignant category moderately differentiated squamous cell carcinoma was the commonest. Maximum numbers of patients were subjected to cervical biopsies in 3 rd decade of life. Malignancies were common in 3 rd & 4 th decade of life. Most of the cases were of parity 4. CONCLUSION : Tissue biopsy is a valuable diagnostic procedure on which surgeons and radiologists still rely.

  15. Clinical study on colposcopy combined with cervical smear in detection of cervical lesions%阴道镜联合宫颈涂片检测宫颈病变的临床研究

    Institute of Scientific and Technical Information of China (English)

    周鸿; 朱瑾

    2012-01-01

    目的:探讨阴道镜联合宫颈涂片检测宫颈病变的临床应用价值.方法:对已婚妇女行宫颈细胞学涂片联合阴道镜检查对宫颈病变进行筛查,以组织病理学诊断为金标准,对结果进行分析.结果:接受宫颈涂片检查的1 030例已婚妇女中有156例行阴道镜下活检病理诊断,其中慢性宫颈炎91例,HPV感染15例,CIN Ⅰ 22例,CINⅡ8例,CINⅢ2例,宫颈癌6例.经阴道镜下RCI评分诊断CIN36例,其中CIN Ⅰ 21例、CINⅡ9例、CINⅢ6例;与阴道镜下活检病理诊断的符合率为94.74%(36/38).宫颈涂片细胞学检查巴氏Ⅲ级以上可疑CIN24例,其中阴道镜下活检病理证实14例,宫颈涂片细胞学检查诊断符合率为58.33% (14/24).阴道镜下RCI评分诊断CIN符合率与宫颈涂片细胞学检查诊断符合率相比差异有统计学意义(P<0.05).统计学处理得出阴道镜诊断CIN的敏感性为95.50%,特异性为89.26%,两者联合的阳性预测值为72.21%,阴性预测值为95.85%.结论:宫颈细胞学涂片联合阴道镜检查筛查子宫颈病变能提高宫颈癌前病变及早期宫颈癌的诊断率,可作为该地区筛查宫颈癌前病变的可行方法.%Objective: To explore the clinical application value of colposcopy combined with cervical smear in detection of cervical lesions. Methods- The married women underwent cervical cytological smear combined with colposcopy to screen cervical lesions, histopatho-logical diagnosis was designed as gold standard, the results were analyzed. Results: Among 1 030 married women undergoing cervical smear, 156 women underwent biopsy under colposcope and pathological examination; 91 cases with chronic cervinitis, 15 cases with human papillo-mavirus (HPV) infection, 22 cases with cervical intraepithelial neoplasia (CIN) 1,8 cases with CIN Q , 2 cases with CIN 1, and 6 cases with cervical cancer were found. Thirty - six cases were diagnosed as CIN by colposcopic RCI score, including 21 cases with

  16. Prevalence and risk factors of non-carious cervical lesions related to occupational exposure to acid mists

    Directory of Open Access Journals (Sweden)

    Rafael Aiello BOMFIM

    2015-01-01

    Full Text Available This study evaluates the prevalence and risk factors of non-carious cervical lesions (NCCLs in a Brazilian population of workers exposed and non-exposed to acid mists and chemical products. One hundred workers (46 exposed and 54 non-exposed were evaluated in a Centro de Referência em Saúde do Trabalhador – CEREST (Worker’s Health Reference Center. The workers responded to questionnaires regarding their personal information and about alcohol consumption and tobacco use. A clinical examination was conducted to evaluate the presence of NCCLs, according to WHO parameters. Statistical analyses were performed by unconditional logistic regression and multiple linear regression, with the critical level of p < 0.05. NCCLs were significantly associated with age groups (18-34, 35-44, 45-68 years. The unconditional logistic regression showed that the presence of NCCLs was better explained by age group (OR = 4.04; CI 95% 1.77-9.22 and occupational exposure to acid mists and chemical products (OR = 3.84; CI 95% 1.10-13.49, whereas the linear multiple regression revealed that NCCLs were better explained by years of smoking (p = 0.01 and age group (p = 0.04. The prevalence of NCCLs in the study population was particularly high (76.84%, and the risk factors for NCCLs were age, exposure to acid mists and smoking habit. Controlling risk factors through preventive and educative measures, allied to the use of personal protective equipment to prevent the occupational exposure to acid mists, may contribute to minimizing the prevalence of NCCLs.

  17. HPV16-E2 induces prophase arrest and activates the cellular DNA damage response in vitro and in precursor lesions of cervical carcinoma.

    Science.gov (United States)

    Xue, Yuezhen; Toh, Shen Yon; He, Pingping; Lim, Thimothy; Lim, Diana; Pang, Chai Ling; Abastado, Jean-Pierre; Thierry, Françoise

    2015-10-27

    Cervical intraepithelial neoplasia (CIN) is caused by human papillomavirus (HPV) infection and is the precursor to cervical carcinoma. The completion of the HPV productive life cycle depends on the expression of viral proteins which further determines the severity of the cervical neoplasia. Initiation of the viral productive replication requires expression of the E2 viral protein that cooperates with the E1 viral DNA helicase. A decrease in the viral DNA replication ability and increase in the severity of cervical neoplasia is accompanied by simultaneous elevated expression of E6 and E7 oncoproteins. Here we reveal a novel and important role for the HPV16-E2 protein in controlling host cell cycle during malignant transformation. We showed that cells expressing HPV16-E2 in vitro are arrested in prophase alongside activation of a sustained DDR signal. We uncovered evidence that HPV16-E2 protein is present in vivo in cells that express both mitotic and DDR signals specifically in CIN3 lesions, immediate precursors of cancer, suggesting that E2 may be one of the drivers of genomic instability and carcinogenesis in vivo. PMID:26474276

  18. Ultrasonic magnetic abrasive finishing

    Institute of Scientific and Technical Information of China (English)

    LU Ya-ping; MA Ji; ZHANG Jun-qiang; WANG Long-shan

    2006-01-01

    Put forward a new kind of polishing method, ultrasonic magnetic abrasive finishing (UMAF), and studied its mechanism of improving polishing efficiency. By analyzing all kind of forces acting on single abrasive particle in the polishing process and calculating the size of the composition of forces, get the conclusion that UMAF will enhance the efficiency of the normal magnetic abrasive finishing(MAF) due to the ultrasonic vibration increases the cutting force and depth. At last the idea of designing the UMAF system based on numerical control milling machine is put forward which is convenient to setup and will accelerate the practical application of MAF.

  19. 宫颈刷片联合阴道镜活检对宫颈癌前病变的筛查分析%Analysis of cervical brush tablets combined with biopsy under colposcopy for screening cervical precancerous lesions

    Institute of Scientific and Technical Information of China (English)

    李迎芳

    2016-01-01

    目的:探讨宫颈刷片联合阴道镜活检对宫颈癌前病变中的筛查作用。方法:选取自愿参加筛查的妇女410例,对其进行宫颈刷片联合阴道镜活检,对有数据进行统计分析。结果:阴道镜下活检组织相符率85.36%。阴道检查结果:CIN76例,CIN Ⅰ级43例,CIN Ⅱ级18例,CIN Ⅲ级9例,癌6例。与病理检查符合率55.26%。结论:宫颈刷片检查联合阴道镜活检能够更有效筛查宫颈癌前变患者,两项联合能够提高宫颈癌早期的诊断率。%Objective:To investigate the effect of cervical brush tablets combined with biopsy under colposcopy for screening cervical precancerous lesions.Methods:410 cases of voluntary screening were selected.All the patients underwentervical brush tablets combined with biopsy under colposcopy.We performed statistical analysis of all data.Results:The colposcopy biopsy conformity rate was 85.36%.Vaginal examination results:76 cases of CIN,CIN Ⅰ in 43 cases,CIN Ⅱ in 18 cases,9 cases of CINⅢ,6 cases of carcinoma.The coincidence rate with pathological diagnosis was 55.26% .Conclusion:The cervical brush tablets combined with biopsy under colposcopy can be more effective in screening of cervical precancerous patients,which can improve the early diagnosis rate of cervical cancer.

  20. Clinical Effect of Percutaneous Nucleoplasty with Radiofrequency Abrasion for Treatment of Cervical Spondylotic Radiculopathy%经皮射频消融术治疗神经根型颈椎病的疗效分析

    Institute of Scientific and Technical Information of China (English)

    张顺聪; 杨志东; 梁德; 江晓兵; 晋大祥; 庄洪; 姚珍松

    2012-01-01

    [目的]探讨经皮射频消融髓核成形术治疗神经根型颈椎病的疗效及安全性.[方法]2008年1月至2010年8月应用经皮射频消融髓核成形术治疗神经根型颈椎病患者27例,比较术前、术后2周及末次随访时的疼痛视觉模拟评分(VAS)和颈部功能障碍指数(NDI),评估临床结果,以及影像学检查颈椎间盘突出、椎体间不稳定等情况,并记录手术并发症.[结果]27例患者总共手术椎间隙37个,其中单节段19例,双节段6例,三个节段2例.随访时间1年~28个月,平均19.8个月.未发现手术节段出现不稳定,未发现手术节段椎间隙出现塌陷.27例患者平均VAS评分为术前(7.84±1.47)分,术后2周减少到(2.44±1.61)分,末次随访为(3.16±1.17)分.平均NDI术前为(61.15±15.71)%,术后2周减少到(16.48±13.29)%,末次随访为(14.42±11.55)%.术后2周及末次随访VAS评分及NDI评分均较术前有显著改善,差异均有统计学意义(P<0.05).无1例患者出现脊髓、神经、大血管损伤及术后感染等并发症;无患者出现脊膜破裂脑脊液漏;无患者出现术中刀头断裂.[结论]经皮射频消融髓核成形术是一种安全的微创手术,如果适应症选择正确,能够有效治疗神经根型颈椎病.%Objective To analyze the clinical outcome and safety of percutaneous nucleoplasty with radiofrenquency abrasion for treatment of cervical spondylotic radiculopathy. Methods From January of 2008 to December of 2010, 27 patients with cervical spondylotic radiculopathy received percutaneous nucleoplasty. The scores of visual ( Continued on page 253)analog scale (VAS) and neck disability questionnaires (NDI) were evaluated before the operation, 2 weeks after the operation, and at the final follow-up. Furthermore, the herniation of intervertebral disk and intervertebral instability were monitored by imageological examination, and all of the intraoperative and postoperative complications were recorded. Results

  1. 宫颈病变筛查策略的效果分析%The Evaluation of the Effect of Cervical Lesions Screening Strategies

    Institute of Scientific and Technical Information of China (English)

    龙腾飞

    2011-01-01

    Cervical cancer is one of the most common female malignant cancers , with an increasing incidence and earlier onset ages. In developed countries, the systematic screening programmes have reduced the morbidity and mortality resulting from cervical cancer. The screening program is the main means to prevent and control cervical cancer. The main screening tools are visual inspection, cytology, human papillomavirus testing and colposcopy. The scholars all over the world made great efforts to seek appropriate cervical cancer screening solutions in either single or comhines use of various methods. Here is to review on the effert of cervical lesions screening strategies in recent years inside and outside China.%宫颈癌是妇科常见的恶性肿瘤之一,其发病率逐年升高,且发病年龄有年轻化的趋势.对宫颈癌的筛查是预防和控制宫颈癌的主要手段,也是极大限度防治宫颈癌的最好途径.宫颈癌筛查方法包括肉眼观察、宫颈细胞学、人类乳头瘤病毒检测及阴道镜检查等.世界各地学者做了许多努力来寻求适宜的宫颈癌筛查方案,包括各种方法的单独及联合使用.现就近年来国内外宫颈病变筛查策略的效果予以综述.

  2. [Unstable traumatic lesions of the lower cervical spine without neurologic deficiency. Our experience at the Fondation Jeanne Ebori in Libreville (Gabon)].

    Science.gov (United States)

    Loembe, P M; Chouteau, Y; Bouger, D

    1991-04-01

    23 cases of unstable traumatic lesions of the lower cervical spine, without neurologic complication, were treated over a period of eight years. These cases consisted of eight dislocations, five subluxations, nine fracture-dislocations and one "tear drop" or fracture of the vertebral body. Six cases were treated conservatively and fifteen operatively. Two patients died in skeletal traction while awaiting surgical intervention. The surgical approaches were anterior (6 cases), posterior (8 cases) and combined (1 case). There was one case of transitory neurologic deficit immediately after surgery. One case required re-intervention. Overall they obtained satisfactory osteoligamentary consolidation. PMID:2055985

  3. Papanicolau smear chances to be diagnostic for cervical squamous intraepithelial lesions (SIL) with or without detectable HPV DNA at in situ hybridization analysis.

    Science.gov (United States)

    Sopracordevole, F; Cadorin, L; Muffato, G; De Benetti, L; Parin, A

    1993-01-01

    The Authors have correlated 39 cervical diagnostic biopsies for squamous intraepithelial lesions (SILs) with correspective Papanicolau smears (PS), with relation to the presence or the absence of HPV of oncogenic type (HPV-one) detected by in situ hybridization (ISH). Agreement between cytological and histological diagnosis was present in 14 of 16 cases with detectable HPV-one and only in 12 of 23 cases without detectable HPV-one at ISH. The importance of the HPV type in the SILs with relation to the diagnostic accuracy of Papanicolaou smears has been discussed.

  4. Human papillomavirus (HPV detected in restored plasma DNA from women diagnosed with pre-invasive lesions and invasive cervical cancer

    Directory of Open Access Journals (Sweden)

    Edward Fabián Carrillo

    2010-06-01

    Full Text Available Objective: To improve the sensitivity of Human Papillomavirus (HPV detection in plasma from high-grade cervical neoplasia patients (CIN III and cervical cancer (CC evaluating any likely correlation with disease stage.Method: We subjected plasma DNA isolates from 112 patients (CIN and ICC to a pre-PCR restoration treatment to improve detection sensitivity. HPV-specific sequences were detected by conventional PCR both in cervical scrapes and plasma DNA obtained from each patient. For every single DNA sample, both non-restored and restored isolates were PCR analyzed.Results: We detected HPV in plasma DNA isolates with significantly higher efficiency on restored plasma-DNA as compared to each non-restored equivalent, still maintaining close correlation with the clinical stage of the cases. By analyzing plasma-DNA isolates we could classify as HPV positive >50.0% of the cases that were previously known to be positive from the cervical scrape based assay. Interestingly, 100% of the cases in which subtype HPV18 was detected in cervical scrapes were also positive in plasma DNA.Conclusions: Restoration of plasma DNA from cervical cancer patients allows a more sensitive PCR-based HPV detection, maintaining the correlation to disease stage traditionally observed.

  5. Research of cervical cytology screening in diagnosis of 370,000 cases with cervical lesion in Guangdong%广东地区37万例妇女子宫颈细胞学筛查子宫颈病变的研究

    Institute of Scientific and Technical Information of China (English)

    Baowen Zheng; Congde Chen; Anxiang Wei; Han Ran; Dong Liu; Wenling Yue; Huirong Zhou; Qingyi Zhu; Juming Su

    2009-01-01

    Objective:Analysis of the results of 37 million cases by using cervical liquid-based cytology screening.discussion the situation of cervical cancer and precancerous lesions in Guangdong province.Methods:From 2002 to 2006.using liquid-based thin-layer cytology (TCT)method screening cervical lesions within 371.929 women in Guangdong.Cytological diagnosis adopt TBS(the Bethesda system)Standard(TBS improved 2001 version of the diagnostic criteria),staastic positive rate by age and region respectively.Results:371,929 cases of gynecology cervical samples in Guangdong.331,251 cases were negative,accounted for 91.23%:cytology positive cases:32,548 cases were squamous intreepithelial lesions(8.96%),486 cases were glandular epithelial lesions(0.13%):grouped according to age:30 to 40-year-old positive rate was 9.13%,40 to 50-year-old positive rate was 9.60%.the latter had higher cytology positive rate;grouped according to region:the total samples in PRD areas were 304.951 cases,accounted for 81.99%,in which 24301 cases were positive(8.14%),in 66978 cases(18.01%)of NON-PRD regions,7645 cases were positive(11.87%),there were 1858 cases had biopsy/follow-up results from 2004t02005.and the rate was 2.88%.Conclusion:The cytological positive rate of cervical cancer and its pre-cancerous lesions was 9.09%in Guangdong(of which the rate of cytological diagnosis as precancerous lesions was 3.40%),30 to 50 age group had the high incidence of cervical cancer and its precursor lesions.and also the main stage of prevention for cervical cancer.Cytology positive rate was 11.87% in economic underdevelopment Non-Pead River Delta regions.much higher than the Pearl River Delta region.

  6. Age trends in the prevalence of cervical squamous intraepithelial lesions among HIV-positive women in Cameroon: a cross-sectional study

    LENUS (Irish Health Repository)

    Atashili, Julius

    2012-10-29

    AbstractBackgroundCervical squamous intra-epithelial lesions (SIL) are more frequent in HIV-positive women overall. However the appropriate age at which to begin and end cervical cancer screening for early detection of lesions in HIV-positive women is not clear. We assessed the age-specific prevalence of any SIL and SIL requiring colposcopy in HIV-positive women in Cameroon.MethodsWe enrolled, interviewed and conducted conventional cervical cytology in 282 women, aged 19--68 years, initiating antiretroviral therapy in three clinics in Cameroon. In bivariable analyses, the crude relationship between age and the presence of lesions was assessed using locally weighted regression (LOWESS) methods. In multivariate analyses, generalized linear models with prevalence as the outcome, an identity link and a binomial distribution, were used to estimate prevalence differences. Bias analyses were conducted to assess the potential effect of inaccuracies in cytology.ResultsSIL were detected in 43.5% of the 276 women with satisfactory samples, 17.8% of whom had ASC-H\\/HSIL. On average, women aged 26 to 59 tended to have a slightly higher prevalence of any SIL than other women (Prevalence difference PD: 6.5%; 95%CI: -11.4, 24.4%). This PD was a function of CD4 count (heterogeneity test p-value =0.09): amongst patients with CD4 counts less than 200cells\\/uL, the prevalence was higher in patients aged 26--59, while there was essentially no difference amongst women with CD4 counts greater than 200 cells\\/uL. ASC-H\\/HSIL were present in women as young as 19 and as old as 62. Overall the prevalence of ASC-H\\/HSIL increased by 0.7% (95%CI: -3.8%, 5.1%) per decade increase in age.ConclusionBoth severe and less severe lesions were prevalent at all ages suggesting little utility of age-targeted screening among HIV-positive women. Nevertheless, the long-term evolution of these lesions needs to be assessed in prospective studies.

  7. Clinical interest of postural and vestibulo-ocular reflex changes induced by cervical muscles and skull vibration in compensated unilateral vestibular lesion patients.

    Science.gov (United States)

    Dumas, Georges; Lion, Alexis; Gauchard, Gérome C; Herpin, Guillaume; Magnusson, Måns; Perrin, Philippe P

    2013-01-01

    Skull vibration induces nystagmus in unilateral vestibular lesion (UVL) patients. Vibration of skull, posterior cervical muscles or inferior limb muscles alters posture in recent UVL patients. This study aimed to investigate the postural effect of vibration in chronic compensated UVL patients. Vibration was applied successively to vertex, each mastoid, each side of posterior cervical muscles and of triceps surae in 12 UVL patients and 9 healthy subjects. Eye movements were recorded with videonystagmography. Postural control was evaluated in eyes open (EO) and eyes closed (EC) conditions. Sway area, sway path, anteroposterior and medio-lateral sways were recorded.A vibration induced nystagmus (VIN) beating toward the healthy side was obtained for each UVL patient during mastoid vibration. In EO, only sway path was higher in UVL group during vibration of mastoids and posterior cervical muscles.The EO postural impairments of UVL patients could be related to the eye movements or VIN, leading to visual perturbations, or to a proprioceptive error signal, providing an erroneous representation of head position. The vibration-induced sway was too small to be clinically useful. Vestibulo-ocular reflex observed with videonystagmography during mastoid vibration seems more relevant to reveal chronic UVL than vestibulo-spinal reflex observed with posturography.

  8. 浙江沿海地区人群TTV和HPV混合感染研究%TTV and HPV co-infection in cervical smears of patients with cervical lesions in littoral of Zhejiang province

    Institute of Scientific and Technical Information of China (English)

    郑美云; 林颖; 李丹军; 阮海波; 陈怡; 吴婷婷

    2010-01-01

    目的 了解浙江沿海地区人群TTV与HPV混合感染状况,探讨TTV传播途径.方法 建立巢式聚合酶链反应方法(nPCR),对健康体检和患宫颈疾病妇女150例宫颈病变细胞学标本及其平行97例血清标本进行TTV DNA及TTV病毒滴度的nPCR检测;应用导流杂交方法检测95例宫颈病变细胞学标本的HPV基因型.结果 TTV DNA在55例健康妇女宫颈细胞标本中检出率52.7%(29/55),与其平行42例血清样本中检出率50.0%(21/42).在患有疾病妇女宫颈细胞中TTV DNA检出率(74.7%)高于健康体检对照组(P=0.005).TTV DNA在患者血清样本中检出率51%(28/55).在宫颈细胞及其平行血清中均检测出TTV基因亚型G1b°TTV病毒滴度在宫颈细胞中高于在其平行血清10~1000倍.HPV在患者组中检出率98.9%(94/95),在健康组中检出率27.3%(15/55).HPV基因型是高危型HPV16、18、33和低危型HPV6.HPV阳性标本TTV DNA检测率明显高于HPV阴性标本(P=0.02).结论 TTV在宫颈细胞中具有高检出率,在宫颈细胞中TTV病毒滴度高于其平行血清.TTV与HPV随性传播感染人群,并在女性生殖道内繁殖.TTV与HPV协同作用有待研究.%Objective To investigate the prevalence of transfusion-transmitted virus (TTV) and human papillomavirus (HPV) co-infection in cervical smears of patients with cervical lesions in littoral of Zhejiang province and analysis of transmitted route.Methods Nested polymerase chain reaction (nPCR) was established.TTV DNA were tested by nPCR in cervical smears of 95 patients with cervical lesions and 55 healthy women,paired serum samples were available from 55 and 42 women,and their viral titer.The genotypes of 95 specimens of cervical cytology were detected with HybriMax.The phylogenetic group of TTV was determined by means of nPCR with N22 primers.Results The prevalence of TTV DNA in cervical smears of patients with cervical lesions and healthy women was 52.7% (29/55) and was comparable with that in paired serum

  9. Clinical Value of LCT and HR-HPV in the Diagnosis of Cervical Lesions%LCT及HR-HPV诊断宫颈病变的临床价值

    Institute of Scientific and Technical Information of China (English)

    李玉环; 戴红英; 闫丽萍; 丁朝霞

    2015-01-01

    Objective To study liquid-based cervical cytology and high risk human papilloma virus testing and bi⁃opsy in the diagnosis, discusses the cervical liquid based cytology and high-risk human papilloma virus detection value to the diagnosis of cervical lesions. Methods Retrospective analysis of the Affiliated Hospital of Qingdao University outpa⁃tient 20479 cases of LCT screening from January 2013 to January 2014. LCT positives were further investigated by HR-HPV testing and colposcopy cervical biopsy pathology, compare the accuracy of the LCT positive results and HR-HPV test results with cervical biopsy and colposcopy results with pathology detection results as the gold standard of diagnosis. Results 1)The consistence rates of ASCUS, LSIL, ASCH, HSIL by LCT and colposcopic multiple biopsies were 54.55%, 62.65%, 88.24%, 92.99%. HR- HPV infection rates of ASCUS, LSIL, ASCH and HSIL respectively were 70.00%, 88.71%, 84.91%, 89.66%. There was seriously statistical significance(P<0.001).2)HR-HPV positive and nega⁃tive groups compared with the high-level cervical lesion detection rates were 48.14%, 17.39%(P<0.001). There was se⁃riously statistical significance. Conclusion Liquid-based cytology and high-risk human papillomavirus detection is an effective screening method for detecting cervical lesions, higher diagnostic accuracy of both joint detection. High-risk hu⁃man papillomavirus detection for cytologic diagnosis of atypical squamous cells of patients has significant shunt value.%目的:探讨LCT和HR-HPV对宫颈病变的诊断价值。方法对20479例患者行LCT检查,LCT阳性者行HR-HPV检测及电子阴道镜下宫颈活检组织病理检查,以组织病理学结果作为诊断的金标准,比较LCT阳性结果、HR-HPV检测结果与阴道镜下宫颈活检病理结果的符合率。结果1)LCT结果为ASCUS、LSIL、ASCH、HSIL与阴道镜下宫颈活检病理结果的符合率分别为54.55%、62.65%)、88.24%、92.99%

  10. The Application Value of Colposcopy Combined with Cervical Cytology in Cervical Lesions%阴道镜检查结合宫颈细胞学在宫颈病变中的应用价值

    Institute of Scientific and Technical Information of China (English)

    王少华

    2014-01-01

    目的:探讨阴道镜检查结合宫颈细胞学在宫颈病变诊治中的临床应用价值。方法随机选在该院接收的656例行阴道镜检查结合宫颈细胞学的妇女作为研究对象,对所有对象的资料进行回顾性分析,总结患者的治疗效果。结果单纯行阴道镜检查筛查3例,无漏诊,癌前期可疑1例。单纯行新柏氏液基细胞学检测检查筛选宫颈癌4例,漏诊1例。行阴道镜检查结合新柏氏液基细胞学检测符合宫颈癌病例5例,无漏诊。结论单纯性阴道镜检查或者新柏氏液基细胞学检测宫颈癌存在漏诊情况,而结合两种方法进行检查后无漏诊现象,即结合两种方法诊断早期宫颈癌有助于提高疾病检出率,值得在临床上推广和使用。%Objective To investigate the clinical application value of colposcopy combined with cervical cytology in the diagnosis and treatment of cervical lesions. Methods 656 cases of women underwent colposcopy combined with cervical cytology in our hos-pital from March, 2012 to March, 2013 were randomly selected as the subjects, and the data of them were analyzed retrospectively, the treatment effect was summarized. Results A simple colposcopy screening 3 cases, no missed diagnosis, pre-cancerous suspi-cious 1 case. Simple ThinPrep liquid-based cervical cytology screening examination in 4 cases, missed 1 case. Colposcopy com-bined with ThinPrep liquid-based cervical cytology in line 5 cases, no missed case. Conclusion Missed diagnosis exists in simple colposcopy or ThinPrep liquid-based cervical cytology for detecting cervical cancer, but that does not exist if combining the two methods for detecting cervical cancer, and the diagnosis of early cervical cancer by combining the two methods is conducive to im-proving the detection rate of the disease, which is worthy of clinical popularization and application.

  11. Risk of Preterm Delivery Associated with Prior Treatment of Cervical Precancerous Lesion according to the Depth of the Cone

    Science.gov (United States)

    Berretta, Roberto; Gizzo, Salvatore; Dall'Asta, Andrea; Mazzone, Eleonora; Monica, Michela; Franchi, Laura; Peri, Francesca; Patrelli, Tito Silvio; Bacchi Modena, Alberto

    2013-01-01

    The aim of this study was to evaluate the impact of the surgical excisional procedures for cervical intraepithelial neoplasia (CIN) treatment both on subsequent fertility (cervical factor) and pregnancy complication (risk of spontaneous preterm delivery). We retrospectively analyzed 236 fertile women who underwent conization for CIN. We included in the study 47 patients who carried on pregnancy and delivered a viable fetus. Patients were asked about postconization pregnancies, obstetrical outcomes, and a possible diagnosis of secondary infertility caused by cervical stenosis. We evaluated the depth of surgical excision, the timing between cervical conization and subsequent pregnancies, surgical technique, and maternal age at delivery. We recorded 47 deliveries, 10 cases of preterm delivery; 8 of them were spontaneous. The depth of surgical excision showed a statistically significant inverse correlation with gestational age at birth. The risk of spontaneous preterm delivery increased when conization depth exceeded a cut-off value of 1.5 cm. Our data do not demonstrated a relation between conization and infertility due to cervical stenosis. PMID:24324288

  12. 人乳头瘤病毒衣壳蛋白与宫颈病变%Human Papillomavirus′ Capsid Proteins and Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    黄成琳; 张淑兰

    2014-01-01

    宫颈癌严重危害妇女健康,人乳头瘤病毒(HPV)感染是其首要病因。临床医师一直致力于寻找一种能有效判断宫颈病变级别及预测预后的诊断方法。 HPV衣壳蛋白包括主要衣壳蛋白(L1壳蛋白)和次要衣壳蛋白(L2壳蛋白),这两种蛋白在组装成病毒颗粒、协助病毒入胞及引起机体免疫反应等多个方面发挥重要作用。近年研究表明, L1壳蛋白可用于预测宫颈病变的进展与消退。以L1及L2壳蛋白为基础研发的HPV预防性疫苗在临床试验中得到了很好的预防效果。综述HPV生物学特点及近年来有关HPV衣壳蛋白在宫颈病变的研究进展。%Cervical cancer seriously endangers women′s health,and human papillomavirus (HPV) is considered to be the primary cause. Doctors have been striving to find an effective diagnostic method for judging cervical lesions level and predicting its prognosis. HPV capsid proteins comprise the major capsid protein (L1 capsid protein) and the minor capsid protein (L2 capsid protein),and these two proteins play an important role in assembling into virus particles,trafficking HPV to the cell,and causing the host′s immune reactions. In recent years,studies have shown that the L1 capsid protein can be used to predict the progress and subsidence of cervical lesions. HPV prophylactic vaccines ,which are exploited on the basis of the L1 and L2 capsid protein,are proved to get a good preventive effect in clinical trials. This paper reviews the biological characteristics of HPV and researches progress on HPV capsid protein in cervical lesions in recent years.

  13. 人乳头瘤病毒衣壳蛋白与宫颈病变%Human Papillomavirus′ Capsid Proteins and Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    黄成琳; 张淑兰

    2014-01-01

    Cervical cancer seriously endangers women′s health,and human papillomavirus (HPV) is considered to be the primary cause. Doctors have been striving to find an effective diagnostic method for judging cervical lesions level and predicting its prognosis. HPV capsid proteins comprise the major capsid protein (L1 capsid protein) and the minor capsid protein (L2 capsid protein),and these two proteins play an important role in assembling into virus particles,trafficking HPV to the cell,and causing the host′s immune reactions. In recent years,studies have shown that the L1 capsid protein can be used to predict the progress and subsidence of cervical lesions. HPV prophylactic vaccines ,which are exploited on the basis of the L1 and L2 capsid protein,are proved to get a good preventive effect in clinical trials. This paper reviews the biological characteristics of HPV and researches progress on HPV capsid protein in cervical lesions in recent years.%宫颈癌严重危害妇女健康,人乳头瘤病毒(HPV)感染是其首要病因。临床医师一直致力于寻找一种能有效判断宫颈病变级别及预测预后的诊断方法。 HPV衣壳蛋白包括主要衣壳蛋白(L1壳蛋白)和次要衣壳蛋白(L2壳蛋白),这两种蛋白在组装成病毒颗粒、协助病毒入胞及引起机体免疫反应等多个方面发挥重要作用。近年研究表明, L1壳蛋白可用于预测宫颈病变的进展与消退。以L1及L2壳蛋白为基础研发的HPV预防性疫苗在临床试验中得到了很好的预防效果。综述HPV生物学特点及近年来有关HPV衣壳蛋白在宫颈病变的研究进展。

  14. Detection of Human Papilloma Virus (HPV in oral mucosa of women with cervical lesions and their relation to oral sex practices

    Directory of Open Access Journals (Sweden)

    Martinez-Martinez Alejandro

    2010-12-01

    Full Text Available Abstract Background Previous studies have either investigated the relationship of HPV with oral cancer or the prevalence of HPV on the oral cavity. The purpose of this investigation was to study the prevalence of HPV in oral cavity of women with oral sex practices and cervical lesions. Methods Forty six (46 non-smokers and non-alcoholic patients attended the "Clínica de Displasias" of "Ciudad Juarez" were sampled. This population had a CIN diagnosis sometime between the previous six months. On previous consent they filled out a questionnaire related to their oral sex practices. Afterwards one swab from cheeks and another from palate/gum were taken; PCR was used to determine generic HPV, HPV16 and HPV18. Results Seventy two percent (72% of the patients stated to have oral sex practices regularly which all of them were positive to HPV either in oral mucus, palate/gum or both. The total of the given results showed that 35% had HPV16; among those distributed in 26% with regular oral sex practices and 9% stated as never practiced oral sex. An association was found between oral HPV16 positivity and progression to cervical CIN advanced lesions. On the other hand HPV18 was not detected. The frequency of HPV16 was higher in buccal mucosa (23% versus palate/gum (16%. Conclusions This study suggests that buccal HPV16 infection is associated with CIN progression.

  15. Association of human papillomavirus, Neisseria gonorrhoeae and Chlamydia trachomatis co-infections on the risk of high-grade squamous intraepithelial cervical lesion

    Science.gov (United States)

    de Abreu, André LP; Malaguti, Natália; Souza, Raquel P; Uchimura, Nelson S; Ferreira, Érika C; Pereira, Monalisa W; Carvalho, Maria DB; Pelloso, Sandra M; Bonini, Marcelo G; Gimenes, Fabrícia; Consolaro, Marcia EL

    2016-01-01

    The link between high-risk human Papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) in the risk of developing cervical cancer still unclear. Thus, in this report we investigated the rates of co-infections between HPV and other important non-HPV STDs in different cervical findings using a multiplex polymerase chain reaction (M-PCR) to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, HSV-1 and -2, and Treponema pallidum. A total of 838 women aged 18 to 68 years were screened using Papanicolaou smears for cervical abnormalities, HPV and non-HPV STDs using PCR and M-PCR methods. A total of 614 (73.3%) of the women had normal cytology (NILM) and 224 (26.7%) women exhibited abnormal cytology (≥ ASC-US). HPV-DNA prevalence was 33.9%, and HPV-16 was the most prevalent genotype in women with NILM and ≥ ASC-US cytology. Non-HPV STDs were detected in 30.4% women and T. vaginalis was the most prevalent one (11.6%). A higher increased risk of ≥ ASC-US and HSIL occurred in co-infections of HR-HPV with C. trachomatis and N. gonorrhoeae. Co-infections of HPV-DNA and HR-HPV with HSV-2 exhibited a similar increased risk but only with ≥ ASC-US. Co-infections of HPV-DNA and HR-HPV with T. vaginalis demonstrated a similar increased risk of ≥ ASC-US and HSIL. We found that C. trachomatis and N. gonorrhoeae were the primary pathogens associated with HR-HPV for the increased risk for all grades of cervical abnormalities but mainly for HSIL, suggesting a possible synergistic action in cervical lesions progression. Our results reinforce the hypothesis that some non-HPV STDs might play a role as co-factors in HPV-mediated cervical carcinogenesis. These data improve our understanding of the etiology of SCC and may also be useful for disease prevention. PMID:27429850

  16. The 836 Cases of Cervical Cancer Screening Lesions Result Analysis%宫颈癌前期病变筛查836例结果分析

    Institute of Scientific and Technical Information of China (English)

    杨惠新

    2012-01-01

    Objective:To summarize and analyze the results of precancerous lesion screening for cervical caner and provide evidence for cervical caner prevention.Methods:The characteristics such as age,education level,birth history and cervical laceration,episiotomy of the 836 participants were reviewed,the results of LCT and histopathologic diagnosis were compared,and the LCT results of foreign female labors and local females were also compared and analyzed.Results:The positive rate of LCT was 10.77%,the accuracy was 89.89%.The proportions of history of birth,episiotomy and cervical laceration in LCT positive participants were statistically significantly higher than in the LCT negative participants (P<0.05).Positive rate of LCT in foreign female labors was statistically higher than in local females (P<0.05). Conclusion:LCT is a ideal screening tool for cervical cancer in this area,gynecologic health care education should be enhanced in foreign female labors to improve the activity and compliance for participating the cervical cancer screening.%目的:总结分析宫颈癌前期病变LCT筛查结果,为本地区宫颈癌预防提供依据.方法:回顾调查836例参加宫颈癌前期病变LCT筛查妇女的年龄、文化程度、分娩史和宫颈撕裂、会阴切除术等病史,对比分析LCT检查及其与组织病理学诊断结果,对比分析外来务工妇女与本地妇女参加检查情况和检查结果.结果:LCT阳性率为10.77%,准确率为89.89%.LCT阳性者,有分娩、会阴切开术、宫颈撕裂史比例高于LCT阴性者,差异具有统计学意义(P<0.05).外来务工妇女LCT检出率高于本地居民,差异具有统计学意义(P<0.05).结论:LCT是本地区较为理想的宫颈癌筛查方法;应大力加强外来务工妇女妇科保健宣传力度,提高其参加宫颈癌筛查的主动性和依从性.

  17. Association of human papillomavirus, Neisseria gonorrhoeae and Chlamydia trachomatis co-infections on the risk of high-grade squamous intraepithelial cervical lesion.

    Science.gov (United States)

    de Abreu, André Lp; Malaguti, Natália; Souza, Raquel P; Uchimura, Nelson S; Ferreira, Érika C; Pereira, Monalisa W; Carvalho, Maria Db; Pelloso, Sandra M; Bonini, Marcelo G; Gimenes, Fabrícia; Consolaro, Marcia El

    2016-01-01

    The link between high-risk human Papillomavirus (HR-HPV) and other sexually transmitted diseases (STDs) in the risk of developing cervical cancer still unclear. Thus, in this report we investigated the rates of co-infections between HPV and other important non-HPV STDs in different cervical findings using a multiplex polymerase chain reaction (M-PCR) to simultaneously detect Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, HSV-1 and -2, and Treponema pallidum. A total of 838 women aged 18 to 68 years were screened using Papanicolaou smears for cervical abnormalities, HPV and non-HPV STDs using PCR and M-PCR methods. A total of 614 (73.3%) of the women had normal cytology (NILM) and 224 (26.7%) women exhibited abnormal cytology (≥ ASC-US). HPV-DNA prevalence was 33.9%, and HPV-16 was the most prevalent genotype in women with NILM and ≥ ASC-US cytology. Non-HPV STDs were detected in 30.4% women and T. vaginalis was the most prevalent one (11.6%). A higher increased risk of ≥ ASC-US and HSIL occurred in co-infections of HR-HPV with C. trachomatis and N. gonorrhoeae. Co-infections of HPV-DNA and HR-HPV with HSV-2 exhibited a similar increased risk but only with ≥ ASC-US. Co-infections of HPV-DNA and HR-HPV with T. vaginalis demonstrated a similar increased risk of ≥ ASC-US and HSIL. We found that C. trachomatis and N. gonorrhoeae were the primary pathogens associated with HR-HPV for the increased risk for all grades of cervical abnormalities but mainly for HSIL, suggesting a possible synergistic action in cervical lesions progression. Our results reinforce the hypothesis that some non-HPV STDs might play a role as co-factors in HPV-mediated cervical carcinogenesis. These data improve our understanding of the etiology of SCC and may also be useful for disease prevention. PMID:27429850

  18. The SNP at −592 of human IL-10 gene is associated with serum IL-10 levels and increased risk for human papillomavirus cervical lesion development

    Directory of Open Access Journals (Sweden)

    Torres-Poveda Kirvis

    2012-11-01

    Full Text Available Abstract Background Women with Human Papilloma Virus (HPV persistence are characterized by high levels of IL-10 at cervix. We have determined whether polymorphisms of IL-10 gene promoter might be associated with increased risk of squamous intraepithelial cervical lesions (SICL and whether exist significative differences of IL-10 mRNA expression at cervix and systemic and serum IL-10 protein between SICL cases and non-Cervical Lesions (NCL. Methods Peripheral blood samples from SICL (n = 204 and NCL (n = 166 were used to detect IL-10 promoter polymorphisms at loci -592A/C (rs1800872, -819C/T (rs1800871, -1082A/G (rs1800896, -1352A/G (rs1800893, by allelic discrimination and to evaluate serum IL-10 protein. Cervical epithelial scrapings from NCL and biopsies from SICLs were used for HPV-typing and to evaluate IL-10 mRNA expression level. The systemic and local IL-10 mRNA expression levels were measured by real time-PCR. Genotypic and allelic frequencies of the selected polymorphisms were analyzed by logistic regression, adjusting by age and HPV-genotype, to determine the association with SICL. Results No significant differences were found between genotype frequencies at loci −819, -1082, and −1352. Individuals carrying at least one copy of risk allele A of polymorphism −592 had a two-fold increased risk of developing SICL [adjusted odds ratio (OR, 2.02 (95% CI, 1.26-3.25, p = 0.003], compared to NCL. The IL-10 mRNA expression and serum IL-10 protein, were significantly higher in SICL cases (p  Conclusions The −592 polymorphism is associated with increased risk of SICL and can serve as a marker of genetic susceptibility to SICL among Mexican women. According to IL-10 levels found in SICL, IL-10 can be relevant factor for viral persistence and progression disease.

  19. Dermoscopy and Onychomycosis: guided nail abrasion for mycological samples*

    Science.gov (United States)

    Bet, Diego Leonardo; dos Reis, Ana Lucia; Chiacchio, Nilton Di; Belda Junior, Walter

    2015-01-01

    Mycological examination is still the cornerstone for the diagnosis of onychomycosis for many dermatologists, but sampling technique interferes on its sensitivity and specificity. Nail abrasion may be used to reach the most proximal part of the lesion and can be easily accomplished with an electric abrasor. We suggest nail plate dermoscopy to identify the best location for localized abrasion to obtain adequate samples for mycological examination. PMID:26734877

  20. Characterization of High Grade Intraepithelial Cervical Lesion among Adolescents and Young Women Caracterización de las lesiones intra-epiteliales cervicales de alto grado en adolescentes y mujeres jóvenes

    Directory of Open Access Journals (Sweden)

    Carlos Eric Daudinot Cos

    2011-11-01

    Full Text Available Introduction: considering the relationship between human papilomavirus and cervical cancer, the current increase in sexually transmitted diseases constitutes the biggest threaten to adolescents’ health. Objective: to characterize high-degree intraepithelial cervical lesion in adolescents and young women. Method: a descriptive and observational study was conducted. It included 52 patients, all of them younger than 24 years old, who had been diagnosed with high-degree cervical pathology and were treated in the cervix pathology consultation of the "Ramón González Coro" Teaching Gynecologic and Obstetric Hospital from January 2007 to December 2008. Risk factors and diagnosis variables were studied and processed using the SPSS 11.5 system for Windows, through the determination of absolute and relative frequencies. Results: 78,8 % of patients had from 20 to 24 years old, 65,3 % of them began having sexual relationships between 15 and 17 years old, 62,2 % had had 3 or more sexual partners and 67,3 % used no contraceptive method. Cyto- histological correlation was of 100 % for intraepithelial cervical lesion II and III. Conclusion: the results of the present study compromise us to highlight the need of extended sexual education among young people.Introducción: el actual incremento de las Infecciones de Transmisión Sexual constituye la mayor amenaza para la salud de los adolescentes, si se conoce la asociación del Virus del Papiloma Humano con el cáncer cervical. Objetivo: caracterizar las lesiones intra-epiteliales cervicales de alto grado en adolescentes y mujeres jóvenes. Método: estudio observacional, descriptivo, en 52 pacientes menores de 24 años con diagnostico de lesión cervical de alto grado, atendidas en la consulta de patología de cuello del Hospital Ginecobstétrico ¨Ramón González Coro¨, de

  1. Targeting immune response with therapeutic vaccines in premalignant lesions and cervical cancer: hope or reality from clinical studies.

    Science.gov (United States)

    Vici, P; Pizzuti, L; Mariani, L; Zampa, G; Santini, D; Di Lauro, L; Gamucci, T; Natoli, C; Marchetti, P; Barba, M; Maugeri-Saccà, M; Sergi, D; Tomao, F; Vizza, E; Di Filippo, S; Paolini, F; Curzio, G; Corrado, G; Michelotti, A; Sanguineti, G; Giordano, A; De Maria, R; Venuti, A

    2016-10-01

    Human papillomavirus (HPV) is widely known as a cause of cervical cancer (CC) and cervical intraepithelial neoplasia (CIN). HPVs related to cancer express two main oncogenes, i.e. E6 and E7, considered as tumorigenic genes; their integration into the host genome results in the abnormal regulation of cell cycle control. Due to their peculiarities, these oncogenes represent an excellent target for cancer immunotherapy. In this work the authors highlight the potential use of therapeutic vaccines as safe and effective pharmacological tools in cervical disease, focusing on vaccines that have reached the clinical trial phase. Many therapeutic HPV vaccines have been tested in clinical trials with promising results. Adoptive T-cell therapy showed clinical activity in a phase II trial involving advanced CC patients. A phase II randomized trial showed clinical activity of a nucleic acid-based vaccine in HPV16 or HPV18 positive CIN. Several trials involving peptide-protein-based vaccines and live-vector based vaccines demonstrated that these approaches are effective in CIN as well as in advanced CC patients. HPV therapeutic vaccines must be regarded as a therapeutic option in cervical disease. The synergic combination of HPV therapeutic vaccines with radiotherapy, chemotherapy, immunomodulators or immune checkpoint inhibitors opens a new and interesting scenario in this disease.

  2. 薄层液基细胞学在宫颈癌及其癌前病变筛查中的价值%The value of Thin prep cytology test in cervical precancerous lesions and cervical cancer screening

    Institute of Scientific and Technical Information of China (English)

    雷静; 万霖; 李惠新; 蒋瑛; 李瑞萍

    2011-01-01

    Objective: To evaluate the detecting technology of thin layers of liquid base cytology (TCT) on the diagnosis of precancerous lesions of uterine cervix and the clinical value, accuracy of the cervical cancer screening. Methods: To collect and analysis 7340 patients who were did examine of TCT in gynecological clinic line of our hospital from May, 2009 ~ November, 2010. Take cytology diagnosed ASC - US and higher as positive results, and the positive results do pathohistological. Take histological diagnosis as gold standard. Results: Thin prep cytology test specimens of SCC, and satisfaction LSIL accuracy HSIL, respectively, 76.8% 97.3%, 100%. Conclusion: TCT combination diagnostic systems of TBS are ideal method of current diagnosis precancerous lesions of uterine cervix (CIN) and cervical cancer screening. Also it can be used as an indicator for cervical cancer detecting. There is some risk of precancerous lesions of young In ASC-US patients.%目的:评价薄层液基细胞学(Thin prep cytology test,TCT)检测技术对宫颈癌前病变的诊断和宫颈癌筛查的准确性及临床价值.方法:收集分析2009年5月~2010年11月在我院妇科门诊行TCT检查的受检者7340例,以细胞学诊断为未明确意义的不典型鳞状上皮细胞(ASC-US)及以上者为阳性结果,并对阳性结果行病理组织学诊断,以组织学诊断作为金标准.结果:液基细胞学标本满意度高,对SCC、HSIL、LSIL的准确率分别为76.8%、97.3%、100%.结论:TCT结合TBS诊断系统是目前诊断宫颈癌前病变和筛查宫颈癌的理想方法 [1],同时也可以作为一项宫颈癌术后随访的检测指标 [2].ASC-US患者中存在部分年轻的高危癌前病变者.

  3. 不同检测方法在宫颈病变和早期宫颈癌筛查中的价值%The Screening Value of Different Detection Methods for Cervical Lesions and Early Cervical Cancer

    Institute of Scientific and Technical Information of China (English)

    张雯; 安红梅; 马丽莎; 邓继红

    2011-01-01

    目的:评价目前临床使用的几种筛查方法在宫颈病变和早期宫颈癌筛查中的价值,针对不同经济、文化及地理背景下的人群,探讨合理、经济、高灵敏度的筛查方案.方法:巴氏涂片、5%醋酸肉眼观察(VIA)、薄层液基细胞涂片法(TCT)结合TBS分类报告系统、HPV-DNA检测(HC-II)、阴道镜检查结合评分系统、镜下定点活检病理组织学检查合理联合应用.结果:1 594例病例进行活检,共检出CINⅡ及CINⅡ以上的宫颈病变65例,其中CINⅡ33例,CINⅢ 31例,浸润癌1例.HC-Ⅱ 诊断高度以上宫颈病变敏感度及约登指数最高分别为92.31%和0.81,TCT、VIA、阴道镜检查和巴氏涂片法诊断宫颈高度以上病变敏感度为中等敏感.HPV-DNA+TCT诊断宫颈高度以上病变敏感度、特异度和约登指数最高.结论:多技术联合互补检查可有效筛查宫颈癌前病变及宫颈癌,医务人员可根据患者的实际情况,为病员选择合适的筛查方案.推荐宫颈病变和早期宫颈癌筛查的最佳方案是同时进行TCT和HPV检测;次佳方案是传统巴氏涂片+HPV检测;一般方案:HPV检测+阴道镜检查;基础方案:巴氏涂片+VIA.%O b jectnve: To evaluate the clinical use of several screening methods for cervical lesions and early cervical cancer and to explore a reasonable choice of economical, high-sensitive screening piogran for the population with different economic, cultural and geographical background M e1hods: Pap smear, visual inspection of 5% acetic acid ( VIA) , thin layer liquid-based cytology smear( TCI) with TBS reporting system, HPV-DNA test( HC-Ⅱ), colposcopy combined scoring system, endoscopic sentinel bbpsy-histological diagnosis, and a reasonable combinatbn of the methods mentioned above were used h this study Results; Totally 1 594 cases undeiwent the biopsy with the results showing65 cases of C IN Ⅱ ormore severe than C IN Ⅱ ( hcluding33 cases of C IN Ⅱ ,31 cases ofCⅢ andl case

  4. Investigation of non-carious cervical lesions of populations in Guangzhou area%广州市中高收入人群楔状缺损调查分析

    Institute of Scientific and Technical Information of China (English)

    闫文娟

    2012-01-01

    Objective To survey the prevalence, characteristics of non-carious cervical lesion and analyze its relationship with life style. Methods Eight hundred and eighty-five subjects including 490 males and 395 females who underwent oral health assessment in Nanfang hospital were surveyed. The shape, size, location of non-carious cervical lesion , tooth brushing habits, hand preference and bruxism were recorded for analysis. Results Non-carious cervical lesions were found in 76. 61% of subjects without gender difference. 70.17% affected teeth demonstrated v-shape lesion. Non-carious cervical lesions were mostly on posterior maxillary teeth and first premolars (34.29% ) were mostly affected. Non-carious cervical lesions were more common in right-hand tooth brusher. Senior patients were more likely to have non carious cervical lesions. Conclusion The prevalence of non-carious cervical lesions in Guangzhou area was high and the knowledge on this disease need to be improved.%目的 调查广州市部分中高收入人群的牙颈部楔状缺损患病率、病变特征及病因,为制定楔状缺损防治措施提供依据.方法 对885名中高收入人员楔状缺损状况进行调查,以楔状缺损牙数、缺损形状、好发牙位、刷牙方式等情况作为统计指标,对所得数据进行统计学分析.结果 该人群楔状缺损患病率为76.61%,男女差异无统计学意义(P>0.05);70.17%的楔状缺损为V形,主要发生在上颌后牙,以上颌第一前磨牙最常见,患病率为34.29%.年龄越大、习惯横向刷牙及爱吃硬食的人,楔状缺损患病率更高.调查人群中楔状缺损的就诊率为20.64%.结论 广州市部分中高收入人群的楔状缺损患病率较高,有喜欢吃硬食、横向刷牙等不良习惯的人群患病率更高.

  5. Risk factors of neurological lesions in low cervical spine fractures and dislocations Fatores de risco de lesão neurológica nas fraturas e luxações da coluna cervical baixa

    Directory of Open Access Journals (Sweden)

    DANILO GONÇALVES COELHO

    2000-12-01

    Full Text Available Eighty-nine patients with lower cervical spine fractures or dislocations were evaluated for risk factors of neurological lesion. The age, sex, level and pattern of fracture and sagittal diameter of the spinal canal were analysed. There were no significant differences on the age, gender, level and Torg's ratio between intact patients and those with nerve root injury, incomplete or complete spinal cord injuries. Bilateral facet dislocations and burst fractures are a significant risk factor of spinal cord injury.O risco de lesão neurológica foi avaliado em 89 pacientes com fraturas ou luxações da coluna cervical baixa. Foram analisados o sexo, a idade, o diâmetro sagital do canal vertebral, o nível e o tipo de fratura ou luxação. Não foram encontradas diferenças significativas para a idade, sexo, nível da fratura ou luxação e razão de Torg entre os pacientes intactos e aqueles com lesão radicular, lesão medular incompleta e lesão medular completa. Luxações bilaterais de facetas articulares e fraturas em explosão são um fator de risco significativo de lesão neurológica.

  6. Prevalence of human papillomavirus types in women with pre-neoplastic and neoplastic cervical lesions in the Federal District of Brazil

    Directory of Open Access Journals (Sweden)

    Geni NL Camara

    2003-10-01

    Full Text Available As a contribution to the public health authorities in planning prophylactic and therapeutic vaccine strategies, we describe the prevalence of human papillomavirus (HPV types in women presenting abnormal cytological results in Pap smear screening tests in the Federal District, Central Brazil. We studied 129 cervical scraping samples from women whose cytological tests showed either pre-neoplastic or neoplastic lesions. Amplification of HPV DNA was performed by polymerase chain reaction using consensus primers MY09 and MY11 followed by identification of isolates by restriction fragment length polymorphism. We detected HPV DNA in 62% of the samples, including HPV-16 in 43.8%, HPV-58 in 12.5%, HPV-31 in 10%, HPV-53 in 6.3%, each of HPV-18 and HPV-33 in 3.8% of the isolates. Other types (HPV-35, -52, -66, -CP8304, -6, -11, and -CP8061 were less frequent (= or < 2.5% each. The prevalence of HPV-58 was relatively higher in this population than in data in South America, but similar to results obtained in other studies in Latin America, Europe, and Eastern Asia. Case-control studies need to be carried out to establish the association between the prevalence of HPV types specially the less frequent high-risk types and cervical cancer.

  7. Cervical Liquid-based Cytology in Cervical Lesions the Pathological Diagnosis of Significance%宫颈液基薄层细胞学在宫颈病变病理诊断中的意义

    Institute of Scientific and Technical Information of China (English)

    郑国文

    2013-01-01

      Objective To investigate the liquid-based cytology (TCT) value in pathological diagnosis of cervical lesions. Methods Retrospective analysis of 2009 January to 2012 June, 5795 patients with TCT cervical cytology, colposcopy biopsy positive cases, compared the two results. Results In 5499 cases of TCT were negative, 296 cases were positive, the positive rate was 5.11%, false negative in 15 cases, no false positive cases. 17 cases of biopsy tissue inflammation, 224 cases of CIN Ⅰ, 20 cases of CINⅡ, 30 cases of CINⅢ, CA 5 cases, LSIL and CIN Ⅰcoincidence rate was 80% (36/45), HSIL and CINⅡ/Ⅲcoincidence rate was 88.9% (24/27). Conclusion TCT examination has a little injury, high positive rate, strong repeatability, for married women can be used as the main method of screening for cervical cancer and precancerous lesions.%  目的探讨液基薄层细胞学(TCT)检查在宫颈病变病理诊断中的意义。方法回顾分析我院2009年1月至2012年6月5795例患者运用 TCT 进行宫颈脱落细胞学检查,阳性病例在阴道镜下活检,二者结果相比较分析。结果 TCT 检查阴性5499例,阳性296例,阳性率5.11%,假阴性15例,无假阳性病例。组织活检炎症17例,CIN Ⅰ224例,CIN Ⅱ20例,CIN Ⅲ30例,CA 5例,LSIL 与 CIN Ⅰ符合率80.0%(36/45),HSIL 与 CIN Ⅱ/Ⅲ符合率88.9%(24/27)。结论 TCT 检查具有损伤小,阳性检出率高,重复性强,对已婚妇女可作为宫颈癌及癌前病变筛查的主要方法。

  8. Study to assess the acceptability and feasibility of cervical cancer screening using visual inspection with acetic acid and treatment of precancerous lesions using cryotherapy in low resource settings

    Directory of Open Access Journals (Sweden)

    Tosha M. Sheth

    2014-06-01

    Full Text Available Background: Objective of current study was to assess the acceptability and feasibility of cervical cancer screening using VIA, and treatment of precancerous lesions using cryotherapy in low resource settings Methods: 526 women from three primary health centers of Shinor taluka (population based approach and 250 women of Medical College, Vadodara (facility based approach were sensitized and screened for cervical cancer. Visual Inspection with Acetic acid (VIA was performed as the screening test. In the population based approach, VIA positive women were referred to a Community Health Centre (CHC for colposcopy and biopsy. Ablative treatment in the form of cryotherapy was offered. Patients requiring higher forms of treatment were referred to medical college, Vadodara. In the facility based approach, VIA positive women underwent colposcopy. Guided biopsy was performed in those with positive lesions on colposcopy. Cryotherapy was offered in the same sitting. Those not suitable for cryotherapy were offered loop electrosurgical excision procedure. Women found to have invasive cancer were offered definitive management. Results: VIA positivity rate was 18.8% in the population based approach and 27.2% in the facility based approach.58.8% women in the population based approach and 77.77% women in the facility based approach were treated with cryotherapy on the same day as screening and none reported any severe side effects. Dropout rate in the community approach was 32.32% whereas in the facility it was 0.4%. Conclusions: VIA and cryotherapy procedures were well tolerated by all screened women. This project has shown that the and ldquo;screen and treat and rdquo; approach can be successfully implemented in the existing health setup. [Int J Reprod Contracept Obstet Gynecol 2014; 3(3.000: 615-621

  9. 人乳头状瘤病毒基因亚型与宫颈病变的关系%The Relationship between Human Papillomavirus Subgenotype and Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    张淑群

    2014-01-01

    Objective To study the relationship between human papilloma virus ( HPV-DNA) subgenotype infection and severity of cervical lesions .Methods 140 cases of HPV infection were selected as the study object .Cervical secretions were taken for viral genotyping ,21 kinds of HPV-DNA subtype analysis was performed by rapid hybridization using nucleic acid mole-cule genotyping technology ,all subjects received lower cervical TCT detection (LCT)and colposcopical multiple biopsy pathology . Under pathological diagnosis ,patients were divided into with normal or inflammation group ,low-grade squamous intraepithelial le-sion (LSIL)group,high-grade squamous intraepithelial lesion (HSIL)group.The relationship between HPV infection and cervical lesions was analyzed according to distribution of HPV-DNA subgenotype .Results 16 kinds of high-risk HPV mRNA isoforms were detected.The common genetic subtypes were HPV-16,58,52,18,33,31,CP8304,and HPV-16 had the highest detection rate.HPV-16 detection rates of the normal or inflammation group ,LSIL group and HSIL group were 19.23%,23.53%,65.22%, detection rate of HPV-16 in HSIL group was significantly higher than those of the other 2 groups .5 kinds of low-risk HPV genetic subtypes were detected,and HPV-11 was the most common.Detection rate of multiple HPV infection in HSIL group was 54.35%, which was significantly higher than that of the other 2 groups,P<0.05,there had statistical significance .Conclusion HPV-16, 58,52,18,33,31,CP8304 infection are the most common in cervical lesions .HPV genotyping assay is of great value for the diag-nosis and prognosis of cervical lesions ,and it is worthy of clinical application .%目的:研究人乳头状瘤病毒基因亚型( HPV-DNA)感染情况及其与宫颈病变程度的关系。方法选取140例HPV感染患者作为临床研究对象。取患者宫颈分泌物作病毒分型,采用核酸分子快速杂交基因分型技术进行21种HPV-DNA亚型分析,所有研究对象均行宫

  10. 子宫颈CIN病变切缘阳性的临床病理因素分析%Clinical pathological factors of margin-positive in cervical CIN lesions

    Institute of Scientific and Technical Information of China (English)

    喇建英; 张凡

    2014-01-01

    Objective:to study the cervical CIN lesions related factors of the cone cutting after cutting edge posi-tive.Methods:A retrospective analysis was performed for vaginal mirror cervical biopsy diagnosis of cervical intraepi-thelial neoplasia(CIN)and accept the cervical cone cut method.All 4527 cases of patients,the patients'age,degree of pathological changes,lesions scope,operation method and whether menopause and the cervical cone cutting and cut-ting edge positive rate for single factor and multiple factors analysis.Results:Multiariable logistic regression analysis found that the operation method is the scope,degree of disease,menopausal status,pathological changes and the cut edge positive highly relevant factors,the relative risk of 7.8735,5.0352,4.1055,4.5571,respectively.Conclusion:Cervical lesion degree of serious,large range of lesions,postmenopausal is cervical LEEP cone cutting operation cut-ting edge positive risk factors ,in the cervical cone cutting operation method choice should attach importance to it.%目的:探讨子宫颈CIN病变锥切术后切缘阳性的相关因素。方法:回顾性分析经阴道镜子宫颈活检诊断为宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)并接受宫颈锥切术的患者4527例,对患者年龄、病变程度、病变范围、手术方式及是否绝经与子宫颈锥切术切缘阳性率作单因素和多因素分析。结果:多因素logistic回归分析发现,手术方式、病变程度、是否绝经、病变范围是与切缘阳性高度相关的因素,相对危险度分别为7.8735、5.0352、4.1055、4.5571。结论:宫颈病变程度严重、病变范围大、LEEP、绝经后是宫颈锥切术切缘阳性的高危因素,在宫颈锥切手术方式选择时应予以重视。

  11. 经阴道彩色多普勒超声在诊断宫颈占位中的价值%Value of Transvaginal Color Doppler Ultrasound in the Diagnosis of Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    李萍; 王丹; 李利琼

    2014-01-01

    Objective To investigate the value of transvaginal color Doppler ultrasound in the diagnosis of patients with cervical lesions. Methods 179 patients with suspected cervical lesions in our hospital from December 2010 to December 2013 were selected in this study. All cases received transvaginal color Doppler ultrasound. All cases were confirmed by operation and histopathological examination, including 114 cases of cervical lesion, and 65 cases of non cervical lesion. The value of transvaginal color Doppler ultrasound in diagnosis of cervical lesions was analyzed. Results The sensitivity, specificity, and accuracy of transvaginal color Doppler ultrasound were 98.2%, 98.5%and 98.3%respectively. 112 patients with cervical lesions diagnozed by transvaginal color Doppler ultrasound included 37 cases of cervical cancer, 23 cases of cervical myoma, 41 cases of cervical polyps, 10 cases of ubmucosal myoma of uterus and one case of cervical pregnancy. Conclusions Transvaginal color Doppler ultrasound is an effective method in diagnosis of cervical lesions, which has high sensitivity, specificity and accuracy. Transvaginal color Doppler ultrasound can provide scientific basis for diagnosis and treatment of patients, which is worthy of clinical application.%目的:探讨经阴道彩色多普勒超声在诊断宫颈占位中的价值。方法选取本院2010年12月至2013年12月疑似宫颈占位病变患者179例,均行经阴道彩色多普勒超声检查,后经手术及病理组织学检查确诊,宫颈占位病变患者114例,非宫颈占位病变患者65例,分析经阴道彩色多普勒超声在诊断宫颈占位中的价值。结果经阴道彩色多普勒超声检查的灵敏度、特异度、准确度分别为98.2%、98.5%、98.3%。经阴道彩色多普勒超声诊断的112例宫颈占位病变患者中,宫颈癌37例、宫颈肌瘤23例、宫颈息肉41例、黏膜下子宫肌瘤10例、宫颈妊娠1例。结论经阴道彩色多普勒超声

  12. Application research of HPV test in screening of cervical lesions%HPV检测在宫颈病变筛查中的应用价值研究

    Institute of Scientific and Technical Information of China (English)

    揭艳频

    2014-01-01

    目的:探讨HPV检测在宫颈病变筛查中的应用价值。方法选择2011年2月-2013年6月本院门诊及体检的500例女性HPV检测的患者,分别给予HPV的TCT和DNA检测,并于病理组织检查结果进行比较。结果随着病变等级的升高, HPV DNA感染率逐渐升高。 TCT检测的阳性率为15.4%,HPV DNA检测的阳性率为35.2%,病理检测的阳性率为19.6%;TCT检测的灵敏度为44.90%,HPV DNA检测的灵敏度为83.67%,两组间差异有统计学意义(P<0.05)。结论 HPV DNA检测的灵敏度高于TCT检测,但两者各有优势,在宫颈病变筛查中的应用价值较高。%Objective To explore the application of HPV test in screening of cervical lesions. Methods 500 cases of female pa-tients with HPV tested in our hospital outpatient service and the physical examination from February 2011 to June 2013were se-lected, they were given the HPV TCT and DNA tested respectively, the examination results was compared with histopathologic. Results With the increase of level of lesion, the HPV DNA infection rate increased. TCT detection positive rate was 15.4%, HPV DNA detection positive rate was 35.2%, the pathological d detection positive rate was 19.6%;TCT detection sensitivity of 44.90%, the HPV DNA sensitivity of 83.67%, the differences between the two groups was statistically significant (P<0.05). Conclusion HPV DNA detection sensitivity is higher than the TCT detection, but they have their own advantages and high application value in screening of cervical lesions.

  13. Research progress in relationship between HPV testing and diagnosis and treatment of cervical lesions%HPV检测在宫颈疾病诊治中的研究进展

    Institute of Scientific and Technical Information of China (English)

    景笑笑; 杨雪峰

    2012-01-01

    Cervical cancer is the most common malignant tumor in female reproductive system, and its etiology is not entirely clear. Currently it is accepted that cervical cancer is related with human papilloma virus ( HPV ) infection. The persistent high - risk HPV infection is the main cause of cervical lesions. The detecting and therapy of high-risk HPV has become an important means in screening precancerous cervical diseases and preventing cervical cancer. This article summarized the relationship between HPV infection and cervical lesions, relationship between HPV viral load and classification and severity of cervical disease, the clinical value of HPV in evaluating therapeutic efficacy, judging prognosis and guiding cytology positive patients in shunt aspects and HPV vaccine.%宫颈癌是女性生殖系统最常见的恶性肿瘤,其发病原因尚未完全清楚,目前公认宫颈癌的发病与人乳头瘤病毒(HPV)感染有关,持续的高危型HPV感染是引起宫颈病变的主要病因,对高危型HPV的检测和治疗已成为筛查宫颈癌前病变、预防宫颈癌的重要手段.该文围绕HPV感染与宫颈病变的关系,HPV病毒载量、分型与宫颈病变严重程度的关系以及其在评估治疗效果、判断预后、指导细胞学阳性患者分流等方面的临床价值,HPV疫苗等的研究进展作以综述.

  14. Clinical significance of Thinprep cytology test in screening of cervical lesions%液基薄层细胞学在筛查宫颈病变中的临床意义

    Institute of Scientific and Technical Information of China (English)

    张江菊; 黄寅虎; 郭建新; 李力; 郑秀惠

    2013-01-01

    Objective:To explore the clinical significance of Thinprep cytology test (TCT) in screening of cervical lesions.Methods:TCT screening was conducted among 18 904 patients from outpatient department of Daping Hospital Affiliated to the Third Military Medical University from January to December in 2012,and 688 patients were found with cytological abnormalities (diagnosis of TBS system ≥ ASC-US),then 416 patients underwent colposcopy and biopsy ; the clinical significance of TCT in screening of cervical lesions was evaluated with pathological examination as gold standard.Results:A total of 416 patients were found with cytological abnormalities,including 195 patients with ASC,65 patients with LSIL,138 patients with HSIL,and 52 patients with cervical cancer; 128 patients with cervicitis,106 patients with cervical intraepithelial neoplasia (CIN) Ⅰ,78 patients with CIN Ⅱ,52 patients with CIN Ⅲ,and 52 patients with cervical invasive carcinoma (including one patient with cervical adenocarcinoma) were found by pathological examination; the coincidence rates of diagnosis between pathological examination and TCT were 48.21% (ASC),81.54% (LSIL),89.13% (HSIL),and 100.00% (cervical cancer),respectively; the sensitivity and specificity of TCT for cervical lesions (≥ CIN Ⅱ) were 90.66% and 76.07%,respectively.Conclusion:TCT can improve the detection rate of cervical lesions and it has a high concordance with the histopathological results with high sensitivity,TCT is an effective method for screening cervical lesions and missed diagnosis can be reduced.%目的:探讨液基薄层细胞学在筛查子宫颈病变中的临床意义.方法:对2012年1月~12月第三军医大学大坪医院18 904例门诊患者进行TCT筛查,发现细胞学异常(TBS系统诊断≥ASC-US)的患者688例,其中416例进行了阴道镜检查并取活检,以病理学为标准,评价TCT筛查子宫颈病变的临床意义.结果:细胞学异常的416例患者中,ASC 195例,LSIL65

  15. ANALYSIS OF COLPOSCOPY EXAMINATION FOR THE CERVICAL CANER AND ITS PRECANCEROUS LESIONS%阴道镜检查在宫颈癌及癌前病变筛查中的分析

    Institute of Scientific and Technical Information of China (English)

    梅天琼; 李静

    2011-01-01

    [Objective] To analyze the effect of colposcopy examination for the cervical caner and its precancerous lesions. [Methods] From June 2009 to December 2011, 835 cases of cervical intraepithelial pathological change, were detected by colposcope and results were compared with biopsy pathology. [Results] The accordance rate of the colposcopy examination and pathology diagnosis diagnosed cervicitis, C1N I , CIN II , CIN HI and SCC were 59.7%, 62.7%, 74.0%, 63.9% and 81.8%. [Conclusion] Colposcopy examination in the diagnosis of cervical disease is more reliable, and with higher sensitivity for cervical cancer, which is valuable for examining cervical cancer and precancerous lesions.%[目的]分析阴道镜检查在宫颈癌及癌前病变筛查中的效果.[方法]2009年6月~2010年12月,将835例阴道镜检查异常者阴道镜检查结果与病理学检查诊断结果间进行对比分析.[结果]阴道镜检查为炎症、CINⅠ、CINⅡ、CINⅢ和SCC者与病理诊断结果比较,诊断符合率分别为59.7%、62.7%、74.0%、63.9%和81.8%.[结论]阴道镜下检查对宫颈疾病的诊断比较可靠,对宫颈癌的敏感性较高,对宫颈癌及癌前病变检查具有较高的临床价值.

  16. Abrasion of ultrafine WC-Co by fine abrasive particles

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Abrasive wear of a series of WC-(5%-14%, mass fiaction)Co hardmetals was investigated employing coarse and fine SiC abrasive under two-body dry abrasion conditions with pin-on-disc and edge-on-disc test arrangements. Unexpectedly, it is found that submicron grades demonstrate substantially higher wear rates comparing with the coarse grades if fine abrasive is utilized in pin-on-disc tests. Such a behavior is attributed to changes in a ratio of abrasive size to size of hard phase as finer abrasive is used.The edge-on-disc test demonstrates that edge wear may be described in two stages with the highest wear rates at the beginning stage.This behavior is associated with a transition of wear mechanisms as edge is wider due to wear. Compared with the ultrafine grades of the same Co content, the coarse grades demonstrate higher wear rates at the beginning, but lower wear rates at the final stage. Wear rates and mechanisms observed at final stage correlate well to the results observed for pin-on-disc tests employing fine abrasive.

  17. Three-year clinical performance of a HEMA-free one-step self-etch adhesive in non-carious cervical lesions.

    Science.gov (United States)

    Van Landuyt, Kirsten L; Peumans, Marleen; De Munck, Jan; Cardoso, Marcio V; Ermis, Banu; Van Meerbeek, Bart

    2011-12-01

    Despite the fact that one-step adhesives are currently used routinely in clinical practice, long-term studies on their clinical performance are scarce. The objective of this randomized controlled clinical trial was to test the hypothesis that a 2-hydroxyethyl methacrylate (HEMA)-free one-step self-etch adhesive performs worse than a conventional multistep etch-and-rinse adhesive. Two-hundred and seventy-six non-carious cervical lesions in 52 patients were restored with a micro-hybrid composite (Gradia Direct; GC). These restorations were bonded in random order either with the HEMA-free one-step adhesive G-Bond (GC) or with the 'gold-standard' (control) three-step adhesive Optibond FL (Kerr). The restorations were evaluated after 6, 12, 24, and 36 months of clinical service regarding retention, marginal adaptation, microleakage, caries occurrence, and sensitivity. After a medium-long period of 3 yr, similar success in clinical performance was observed for the simplified all-in-one adhesive and the conventional three-step adhesive. However, the one-step adhesive exhibited significantly more incisal marginal defects and discolorations. Whereas marginal degradation appeared to arrest for the multistep etch-and-rinse adhesive after 12 months, the enamel margins of the restorations bonded with the one-step self-etch adhesive continued to deteriorate. These incisal marginal defects were, however, small and could easily be removed by polishing. For both adhesives, large and sclerosed lesions appeared to be at higher risk of retention loss. PMID:22112039

  18. Human Papillomavirus and Cervical Cancer

    OpenAIRE

    D. Jenkins(University of York, UK)

    2003-01-01

    Of the many types of human papillomavirus (HPV), more than 30 infect the genital tract. The association between certain oncogenic (high-risk) strains of HPV and cervical cancer is well established. Although HPV is essential to the transformation of cervical epithelial cells, it is not sufficient, and a variety of cofactors and molecular events influence whether cervical cancer will develop. Early detection and treatment of precancerous lesions can prevent progression to cervical cancer. Ident...

  19. Application of liquid -based cytology with colposcopy in diagnosis of cervical lesions%液基细胞学结合阴道镜检查在诊断宫颈病变中的应用

    Institute of Scientific and Technical Information of China (English)

    周晓花

    2011-01-01

    Objective To explore and evaluate the liquid -based cytology(TCT) and colposcopy for cervical lesions.Methods The diagnostic criteria for pathological results in our hospital colposcopy, biopsy and the cervical lesions TCT test results were analyzed.Results TCT compared with the biopsy tissue, benign coincidence rate was 98.4% , LSIL, HSIL, cancer diagnosis rate was 85.4% ,97% and 100%; Colposcopy compared with histologica findings, CIN or more colposcopy cervical lesions was 97.8% compliance.Conclusions TCT consistent with colposcopy was no significant difference( P > 0.05 ), application of TCT assisted colposcopy can improve cervical lesions and cancer detection rate.%目的 探讨和评价液基细胞学(TCT)结合阴道镜对宫颈病变的诊断价值.方法 以病理学结果为诊断标准,对在永城市人民医院进行阴道镜、活检及TCT的宫颈病变患者的检查结果进行分析.结果 TCT与活检病理组织学相比,良性符合率为98.4%,LSIL、HSIL、癌的诊断符合率分别为85.4%、97%和100%;阴道镜检查结果与组织学相比,CIN以上的宫颈病变阴道镜符合率为97.8%.结论 TCT与阴道镜检查符合率无统计学意义(P>0.05),应用TCT辅助阴道镜检查可提高宫颈癌前病变和癌变的检出率.

  20. One year comparative clinical evaluation of EQUIA with resin-modified glass ionomer and a nanohybrid composite in noncarious cervical lesions

    Directory of Open Access Journals (Sweden)

    Deepa Sunil Vaid

    2015-01-01

    Full Text Available Aims: Comparative evaluation of EQUIA with a resin-modified glass ionomer cement (RMGIC; GC Gold Label glass ionomer light cured universal restorative cement and a nanohybrid composite (Tetric N-Ceram in noncarious cervical lesions (NCCLs. Background: To establish the most suitable material for the restoration of NCCLs. Settings and Design: In vivo study. Materials and Methods: Eighty-seven NCCLs were randomly restored with EQUIA, a RMGIC, and a nanohybrid composite. Clinical evaluation of the restorations was done following the Unites States Public Health criteria by a single-blinded investigator. Data were formulated, and statistical analysis was done by Chi-square test. Statistical Analysis Used: Chi-square test. Results: No significant difference was found between EQUIA, RMGIC, and nanohybrid composite at 1-month, at 6 months, and at 1-year (P > 0.05. Conclusions: EQUIA, resin-modified glass ionomer, and nanohybrid composite performed equally at 1-month, 6 months, and 1-year follow-up periods.

  1. 多种HPV亚型检测在宫颈病变诊治中的意义%Significances of detections of multiple human papillomavirus subtypes in diagnosis and treatment of cervical lesions

    Institute of Scientific and Technical Information of China (English)

    李娟; 尹格平; 陈铭; 朱彤宇; 崔晓宁; 提松梅

    2012-01-01

    目的:通过研究不同HPV亚型与宫颈病变之间的关系,为宫颈病变的筛查和防治提供理论基础.方法:采用导流杂交基因芯片技术对1 430例宫颈病变患者进行21种HPV亚型检测,包括16种高危亚型(HR - HPV)和5种低危亚型(LR- HPV),对阳性患者行宫颈多点活体组织检查(活检),以组织病理学诊断作为宫颈病变确诊的金标准.结果:①1 430例宫颈病变患者中检测到HPV阳性患者210例,阳性率为14.68%,其中,感染HR - HPV 174例(82.86%),感染LR - HPV 36例(17.14%);居前6位的感染亚型是:HPV 16 (43.81%)、52 (15.71%)、11 (10.00%)、58 (8.57%)、31 (7.62%)、33(7.14%).其中单一感染176例(83.81%),多重感染34例(16.19%).②HR - HPV感染致CIN和宫颈癌的发病率明显高于LR - HPV; HR - HPV的感染率随宫颈病变程度的加重而逐渐增高,其中,宫颈癌组HR - HPV感染率与对照组比较差异有统计学意义(P<0.05).③宫颈癌组HPV多重感染率与对照组比较差异有统计学意义(P<0.05).结论:不同HPV亚型感染可导致不同的宫颈病变,并且与宫颈病变的不同程度密切相关.HPV多重感染与宫颈癌的发病密切相关.多种HPV亚型检测在宫颈病变筛查和防治过程中具有重要意义.%Objective: To provide theoretical basis for screening, prevention, and treatment of cervical lesions by investigating the relationship between different human papillomavirus ( HPV) subtypes and cervical lesions. Methods; Flow - through hybridization gene chip technique was used to detect 21 kinds of HPV subtypes among 1 430 patients with cervical lesions, 16 kinds of high risk HPV subtypes and 5 kinds of low risk HPV subtypes were included, the positive patients received multiple punch cervical biopsy, histopathological diagnosis was used as gold standard of cervical lesions. Results; Among 1 430 patients with cervical lesions, 210 patients were found with HPV, the positive rate was 14. 68

  2. Clinical Value of Liquid-based Cytology in the Diagnosis of Cervical Lesions%液基细胞学检查在诊断宫颈病变中的临床价值

    Institute of Scientific and Technical Information of China (English)

    姜丽红; 杨滨

    2011-01-01

    [目的]探讨宫颈液基细胞学检查(TCT)对宫颈病变的诊断价值及意义.[方法]回顾性分析中国医科大学附属盛京医院门诊57 784例TCT筛查结果.细胞学诊断采用TBS(2001)分级报告系统,阳性诊断包括意义不明确的不典型鳞状细胞(ASC-US)及以上病变,其中阳性者554例行阴道镜下宫颈活组织检查,其中317例同时又进行了宫颈高危型人乳头瘤病毒DNA(HPV-DNA)检测,对三者进行了对比分析.另外对经宫颈活检证实为宫颈癌并同时有TCT检测结果的103例患者结果也进行了对比分析.[结果]①TCT检查阳性结果与宫颈活检诊断符合率:鳞状细胞癌(SCC)3例及腺癌4例均符合组织病理学诊断;高级别鳞状上皮内病变(HSIL)符合率为86.67%(91/105),低级别鳞状上皮内瘤变(LSIL)符合率为33.93% (38/112);②经宫颈活检证实为原位癌和SCC患者TCT与宫颈活检诊断的符合率为28.57%(26/91),假阴性率38.46%(35/91),经宫颈活检证实为腺癌患者TCT与宫颈活检诊断的符合率为50.00%(6/12),假阴性率41.67%(5/12);③TCT提示HPV感染与宫颈HPV-DNA检测的符合率为79.00%(173/219);④宫颈高危型HPV-DNA 阳性检出率与组织病理学分级正相关.[结论]TCT用于宫颈病变筛查,能够及时发现大量的宫颈病变,TCT结果为HSIL及宫颈癌的患者与宫颈活检诊断的符合率较高.TCT提示HPV感染有较高的准确性,但TCT对宫颈癌筛查有较高的假阴性率.对于TCT检查阴性、而临床高度可疑恶性病变、年龄较大的患者应予以重视,进一步做阴道镜检查及高危型HPV-DNA检测和宫颈活组织检查,尤其是宫颈管诊刮,以降低宫颈癌的漏诊率.高危型HPV-DNA检测可以作为TCT诊断的客观控制指标,还可作为ASC-US及LSIL患者分流无病与患病人群的检查.%[Objective] To explore the value of liquid-based cervical cytology(TCT) for the diagnosis of cervical lesions and its significance. [Methods] The TCT

  3. Clinical effectiveness of a one-step self-etch adhesive in non-carious cervical lesions at 2 years.

    Science.gov (United States)

    Ermis, R Banu; Van Landuyt, Kirsten L; Cardoso, Marcio Vivan; De Munck, Jan; Van Meerbeek, Bart; Peumans, Marleen

    2012-06-01

    A 2-year randomized, controlled prospective study evaluated the clinical effectiveness of a one-step self-etch adhesive and a "gold-standard" three-step etch-and-rinse adhesive in non-carious Class-V lesions. The null hypothesis tested was that the one-step self-etch adhesive does perform clinically equally well as the three-step etch-and-rinse adhesive. A total of 161 lesions in 26 patients were restored with Clearfil AP-X (Kuraray). The restorations were bonded either with the "all-in-one" adhesive Clearfil S3 Bond (Kuraray) or with the three-step etch-and-rinse adhesive Optibond FL (Kerr). The restorations were evaluated at baseline and after 6 months, 1 and 2 years, regarding their retention, marginal adapation, marginal discoloration, caries occurrence, preservation of tooth vitality and post-operative sensivity. Retention loss, severe marginal defects and/or discoloration that needed intervention (repair or replacement) and the occurrence of caries were considered as clinical failures. The recall rate at 2 years was 93.8%. Only one Clearfil S3 Bond restoration was lost at the 2-year recall. All other restorations were clinically acceptable. The number of restorations with defect-free margins decreased severely during the 2-year study period (to 6.7% and 25.3% for Clearfil S3 Bond and Optibond FL, respectively). The Clearfil S3 Bond restorations presented significantly more small marginal defects at the enamel side than the Optibond FL restorations (Clearfil S3 Bond: 93.3%; Optibond FL: 73.3%; p = 0.000). Superficial marginal discoloration increased in both groups (to 53.3% and 36% for Clearfil S3 Bond and Optibond FL, respectively) and was also more pronounced in the Clearfil S3 Bond group (p = 0.007). After 2 years, the simplified one-step self-etch adhesive Clearfil S3 Bond and the three-step etch-and-rinse adhesive Optibond FL were clinically equally successful, even though both adhesives were characterized by progressive degradation in marginal

  4. Serial type-specific human papillomavirus (HPV) load measurement allows differentiation between regressing cervical lesions and serial virion productive transient infections

    International Nuclear Information System (INIS)

    Persistent high-risk human papillomavirus (HPV) infection is strongly associated with the development of high-grade cervical intraepithelial neoplasia (CIN) or cancer. Not all persistent infections lead to cancer. Viral load measured at a single time-point is a poor predictor of the natural history of HPV infections. However the profile of viral load evolution over time could distinguish nonprogressive from progressive (carcinogenic) infections. A retrospective natural history study was set up using a Belgian laboratory database including more than 800,000 liquid cytology specimens. All samples were submitted to qPCR identifying E6/E7 genes of 18 HPV types. Viral load changes over time were assessed by the linear regression slope. Database search identified 261 untreated women with persistent type-specific HPV DNA detected (270 infections) in at least three of the last smears for a average period of 3.2 years. Using the coefficient of determination (R²) infections could be subdivided in a latency group (n = 143; R² < 0.85) and a regressing group (n = 127; R² ≥ 0.85). In (≥3) serial viral load measurements, serial transient infections with latency is characterized by a nonlinear limited difference in decrease or increase of type-specific viral load (R² < 0.85 and slopes between 2 measurements 0.0010 and −0.0010 HPV copies/cell per day) over a longer period of time (1553 days), whereas regression of a clonal cell population is characterized by a linear (R² ≥ 0.85) decrease (−0.0033 HPV copies/cell per day) over a shorter period of time (708 days; P < 0.001). Using serial HPV type-specific viral load measurements we could for the first time identify regressing CIN2 and CIN3 lesions. Evolution of the viral load is an objective measurable indicator of the natural history of HPV infections and could be used for future triage in HPV-based cervical screening programs

  5. 孕期雌激素水平在宫颈癌早期病变中的影响%The Influence of Estrogen Level during Pregnancy in Cervical Cancer Early Lesion

    Institute of Scientific and Technical Information of China (English)

    陆建军

    2015-01-01

    Objective:To analyze the effect of estrogen level during pregnancy in cervical cancer early lesion.Method:220 cases of postpartum outpatients with cervical liquid-based cytology technology (LCT) and 250 cases of cervical cancer screening in our hospital from March 2012 to December 2014 were randomly selected,and then analyzed the pathlogy reports.Result:The positive rate of postpartum outpatients group was 10.0%,the cervical cancer screening group was 5.2%.The difference was statistically significant(P<0.05).Conclusion:Elevated level of estrogen has promoting effect in the diagnosis of cervical cancer early lesion.%目的:分析孕期妇女雌激素变化在宫颈癌早期病变中的影响。方法:随机选择2012年3月-2014年12月笔者所在医院220例产后门诊与同期250例两癌筛查项目中宫颈癌筛查的液基细胞学检查,并分析病理报告。结果:产后门诊组阳性率为10.0%,同期宫颈癌筛查组阳性率为5.2%,差异有统计学意义(P<0.05)。结论:孕期雌激素的剧烈变化对宫颈癌早期病变诊断有促进作用。

  6. Emprego da submucosa de intestino delgado porcina no reparo de lesões do esôfago cervical. Estudo experimental em cães Small intestine submucosa for repair of cervical esophageal lesions in a dog model

    Directory of Open Access Journals (Sweden)

    Zacarias Alves de Souza Filho

    2003-06-01

    Full Text Available OBJETIVO: Pesquisar a eficácia da submucosa de intestino delgado (SID porcina como enxerto para reparo de lesões em esôfago cervical de cães. MÉTODOS: Foram operados 10 cães. Ressecou-se parcialmente uma porção da parede anterior do esôfago que foi substituída por um enxerto de SID. Avaliaram-se a resistência tensiométrica, o aspecto macroscópico, a regeneração tecidual e a concentração de colágeno da porção esofágica onde se implantou o enxerto. RESULTADOS: Não houve infecção, fístula ou estenose. Verificou-se ganho semelhante de resistência do esôfago operado em relação ao controle: 31.84N contra 28.60N em média (p=0,593. A macroscopia revelou cicatrização completa com pouca retração tecidual. O estudo anátomo-patológico por HE mostrou re-epitelização completa da mucosa, proliferação vascular discreta a moderada e proliferação fibroblástica intensa. Na análise do colágeno pelo Sirus-red obteve-se em média 54.04% de colágeno tipo I, 16,04% de colágeno tipo III e 71.58% de colágeno total. CONCLUSÃO: A SID mostrou ser, no cão, um enxerto eficaz no reparo de lesões maiores do esôfago, apresentando-se resistente à infecção e à rejeição. A SID deve ser, portanto, considerada opção importante no tratamento destas lesões.PURPOSE: Study of porcine small intestinal submucosa (SIS efficiency as a graft in the handling of lesions of the cervical esophagus in dogs. METHODS: Ten dogs were operated. A resection of a portion of the anterior wall of the esophagus was performed, followed by its repair with a SIS graft. The tensiometric resistance, macroscopic appearance regeneration process and collagen contents of the esophagus tissue were evaluated. RESULTS: No cases of fistula, stenosis or infection occured. Tensiometric evaluation showed a similar resistance in the operated esophagus when compared to the control (an average of 31.84N against 28.60N. Macroscopic analysis revealed complete healing

  7. 阴道镜联合宫颈锥切对宫颈癌及其癌前病变患者的诊断价值分析%Diagnostic Value of Colposcopy and Cervical Conization for Cervical Cancer and Precancerous Lesion

    Institute of Scientific and Technical Information of China (English)

    易黎; 归倩

    2014-01-01

    Objective To explore the diagnostic value of colposcopy and cervical conization for cervical cancer and pre -cancerous lesion .Methods 618 cases with cervical cancer and precancerous lesion were selected as research subjects ,including 32 cases of CINⅠ,58 cases of CINⅡ,40 cases of CIN Ⅲ and 8 cases of cervical cancer .Results The sensitivity,specificity, positive predictive value and negative predictive value of colposcopy for cervical cancer and precancerous lesion were 74.6%, 93.8%,77.4%and 92.8%,respectively;The rates of correct diagnosis of colposcopy for CIN Ⅰ,CIN Ⅱ and CIN Ⅲ were 59.4%(19 cases),79.3%(46 cases) and 82.5%(33 cases);22 cases of CINⅠ,50 cases of CINⅡand 36 cases of CIN Ⅲwere detected by cervical conization;5 cases (62.5%) and 2 cases(25.0%) of cervical cancer were detected by colposcopy or cervical conization respectively .Combined diagnostic rate of colposcopy and cervical conization was 87.5%.Conclusion Colpos-copy and cervical conization improve the diagnostic rate of cervical cancer and precancerous lesion .%目的:探讨阴道镜联合宫颈锥切( LEEP)对宫颈癌及其癌前病变患者的诊断价值。方法选择618例宫颈癌及癌前病变患者为研究对象,其中病理诊断确诊宫颈上皮内瘤变( CIN)Ⅰ级32例,Ⅱ级58例,Ⅲ级40例,宫颈浸润癌8例。采用宫颈锥切治疗,分析手术前后病理诊断情况。结果在618例患者中,阴道镜诊断宫颈癌及癌前病变的灵敏度、特异度、阳性预测值及阴性预测值分别为74.6%、93.8%、77.4%和92.8%;阴道镜正确诊断CINⅠ级、CINⅡ级及CINⅢ级分别为19例(59.4%)、46例(79.3%)及33例(82.5%);子宫锥切术检出CINⅠ级22例,Ⅱ级50例,Ⅲ级36例;阴道镜诊断宫颈浸润癌5例(62.5%),经宫颈锥切另发现2例(25.0%),两者联合诊断宫颈浸润癌准确率为87.5%。结论阴道镜联合宫颈锥切可

  8. The effect of human papillomavirus DNA testing and cervical cytology for screening of cervical precancerous lesions%人乳头瘤病毒DNA检测和宫颈细胞学检查对宫颈癌前病变筛查的效果

    Institute of Scientific and Technical Information of China (English)

    梁瑜; 彭华丽

    2015-01-01

    Objective To investigate the effect of human papillomavirus (HPV) DNA testing and cervical cytology screening of cervical precancerous lesions. Methods 1100 patients admitted to our hospital who were carried out screening cervical precancerous lesions from January 2013 to June 2014 were selected,all were carried out HPV DNA testing,cervical cytology,colposcopy and cervical biopsy,pathological diagnosis was as the standard,the test results were analyzed. Results Positive rate of HPV DNA testing was 23.00% in 1100 patients,in which,positive rate of cervical cancer was 83.33%,positive rate of cervical intraepithelial neoplasia (CIN) Ⅰ was 44.44%,CIN II testing was 86.67%, CINⅢ positive rate was 94.12%.Cervical cytology results showed that there were 851 cases (77.36%) with no CIN,139 cases (12.64%) were atypical squamous cells,78 cases (7.09%) were low-grade squamous intraepithelial lesion,29 cases (2.64%) of high-grade squamous intraepithelial cells lesions,3 cases (0.27%) of squamous cell carcinoma.The compli-ance rate of colposcopy examination and pathological diagnosis was 62.79% (54/86). Conclusion The suitable cervical lesions screening method for patients can help to improve the detection rate,reduce the incidence rate of cervical cancer.%目的:探讨人乳头瘤病毒(HPV)DNA检测和宫颈细胞学检查对宫颈癌前病变筛查的效果。方法选取2013年1月~2014年6月来本院就诊并进行宫颈癌前病变筛查的1100例患者,均进行HPV DNA检测、宫颈细胞学检查、阴道镜检查与宫颈活检,以病理诊断为标准,对检测结果进行分析。结果1100例患者中,HPV DNA阳性率为23.00豫,其中宫颈癌阳性率为83.33豫,宫颈上皮内瘤变(CIN)Ⅰ阳性率为44.44豫,CINⅡ阳性率为86.67豫,CINⅢ阳性率为94.12豫。宫颈细胞学检查无CIN 851例(77.36豫),非典型鳞状上皮细胞139例(12.64豫),低度鳞状上皮细胞内病变78例(7.09豫),高度鳞状上皮细胞内病变29

  9. 深入分析LEEP活检术在宫颈病变中的诊断价值%In-depth analysis of the diagnostic value of LEEP biopsy in cervical lesions

    Institute of Scientific and Technical Information of China (English)

    吴悦; 孙跃峰

    2014-01-01

    Objective to study the application value of leeP biopsy in patients with cervical lesions. Methods the data of 31 cases of patients with cervical lesions in our hospital were retrospectively analyzed. they were detected by liquid-based cytology test of multiple biopsies with colposcopy showing low diagnostic rate and also they were positive HPV test patients. then they were tested by leeP biopsy.Results The ifndings in patients with cervical lesions for LEEP biopsy showed four cases with inlfammation, 5 cases with low-grade squamous intraepithelial lesions of CNⅠ, 10 cases of cnⅡ, 8 cases of cnⅢ, 1 case with squamous cell carcinoma, three cases with adenocarcinoma. also, of 31 cases with positive HPV test, 12 cases of low-risk patients, 19 cases of high-risk patients. The high-risk rates were:inlfammation 0, 40%of low-grade squamous intraepithelial lesions of cnⅠ, 75%of cnⅡ, 87.5%of cnⅢ, 100%of squamous cell carcinoma, adenocarcinoma 0. Conclusion leeP biopsy accurately diagnoses patients with cervical lesions, which can be widely distributed in clinical applications.%目的:研究应用leeP活检术在宫颈病变患者检查中的应用价值。方法我院的31例宫颈病变患者的案例进行回顾性分析,31例宫颈病变患者均为阴道镜下多点活检比液基薄层细胞检测结果低诊且HPV检测阳性的患者,进行leeP活检。结果经leeP活检的宫颈病变患者的检查结果为:炎症4例,鳞状上皮内低度病变cnⅠ5例,cnⅡ10例, cnⅢ8例,鳞状细胞癌1例,腺癌3例;31例HPV检测阳性患者中,低危型12例患者,高危型19例患者,各项高危率分别为:炎症0,鳞状上皮内低度病变cnⅠ40%,cnⅡ75%,cnⅢ87.5%,鳞状细胞癌100%,腺癌0。

  10. Associação entre o polimorfismo no códon 72 da p53 e as lesões pré-malignas e malignas cervicais Association of codon 72 polymorphism with premalignant and malignant cervical lesions

    Directory of Open Access Journals (Sweden)

    Fernando Anschau

    2005-10-01

    Full Text Available OBJETIVOS: testar a hipótese de que o polimorfismo no códon 72 do gene TP53 é fator de risco para as lesões pré-malignas e malignas cervicais associadas ou não ao papilomavírus humano (HPV. MÉTODOS: foram incluídas amostras de cérvice uterina, para pesquisa de DNA de HPV e do polimorfismo no códon 72 da p53 com o uso da reação em cadeia da polimerase (PCR, de 155 pacientes que se submeteram à biópsia cervical. Foram formados três grupos de acordo com o diagnóstico histológico: lesão escamosa intra-epitelial de baixo grau (LSIL, lesão escamosa intra-epitelial de alto grau (HSIL e carcinoma cervical. Aquelas pacientes sem alterações displásicas, citológicas e histológicas, foram consideradas controles. Para testar a associação entre o polimorfismo no códon 72 do gene TP53 e os grupos, foi utilizado o teste de chi2. Considerou-se como significativo o intervalo de confiança no nível de 95% (alfa=0,05. RESULTADOS: quarenta pacientes tiveram o diagnóstico histológico de carcinoma cervical, 18 tinham HSIL, 24 tinham LSIL e 73 foram consideradas controles. O genótipo Arg/Arg p53 foi encontrado em 60,0% das pacientes com câncer, 50,0% dos casos com HSIL, 45,8% dos casos com LSIL e em 45,2% dos controles. Não houve diferença significativa entre as proporções de cada genótipo da p53 nos diferentes grupos independente da presença do HPV (chi2: 3,7; p=0,716. CONCLUSÕES: nossos dados não suportam a hipótese de que o polimorfismo no códon 72 do gene TP53 é importante no desenvolvimento de lesões cervicais pré-malignas e malignas associadas ou não ao HPV.PURPOSE: to test the hypothesis that gene TP53 codon 72 polymorphism is a risk factor for premalignant and malignant cervical lesions associated or not with human papillomavirus (HPV. METHODS: uterine cervical samples were collected for HPV DNA and TP53 codon 72 polymorphism tests from 155 patients who underwent cervical biopsy. Three groups were formed according

  11. 电子阴道镜下定位活检诊断宫颈病变的临床意义%Clinical significance of diagnosis of cervical lesions under colposcopy biopsy

    Institute of Scientific and Technical Information of China (English)

    张敖

    2011-01-01

    目的 探讨电子阴道镜下定位活检诊断宫颈病变的临床意义.方法 扬州市妇幼保健院2006年12月至2008年11月经液基细胞学诊断为非典型鳞状细胞(atypicalsquamous cells,ASC)及其以上患者229例,应用电子阴道镜定位活检,评价电子阴道镜下诊断宫颈病变的价值.结果 本组经TCT筛查的细胞学阳性患者229例,其中ASCUS69例,LSIL124例,HSIL31例,SCC5例;经阴道镜下定位活检结果为宫颈炎70例;CIN 153例.其中CIN I 101例,CINII39例,CINIII 13例;浸润癌6例.结论 阴道镜下对子宫颈进行定位活检可提高CINII和CINⅢ及早期子宫颈癌的准确性和检出率,而对于子宫颈癌前期病变,尤其是早期子宫颈癌的诊断有重要的临床价值.%Objective To investigate the electronic colposcopic biopsy diagnosis of cervical lesions of clinical significance. Methods The hospital in December 2006 - November 2008 for the liquid - based cytology atypical squamous cells (atypicalsquamous cells, ASC) and above were 229 cases , the application of colposcopy biopsy, evaluation of electronic colposcopy and the value of the diagnosis of cervical lesions. Results The cytological screening by the TCT - positive patients 229 cases, of which ASCIIS 69 case, LSIL 124 cases, HSIL31 cases, SCC5 cases; by colposcopic biopsy results cervicitis 70 cases; CIN 153 cases, including CIN I 101 cases, CINII39 cases, CINIII 13 cases; invasive carcinoma in 6 cases. Conclusions Colposcopic biopsy of the cervix can be improved CINII and CIN M and early cervical cancer detection rate and accuracy, and for cervical cancer precursor lesions , especially in the early diagnosis of cervical cancer has important clinical value.

  12. AgNOR polymorphism association with squamous intraepithelial lesions and invasive carcinoma with HPV infection Asociación de los polimorfismos AgNORs con lesiones intraepiteliales escamosas, carcinoma cervical e infección por VPH

    Directory of Open Access Journals (Sweden)

    Luz del Carmen Alarcón-Romero

    2009-04-01

    Full Text Available OBJECTIVE: Evaluate the relationships between AgNORs polymorphisms and squamous intraepithelial lesions (SIL and squamous cell carcinoma (SCC with HPV infection. MATERIALS AND METHODS: A study was carried out on sixty women from the state of Guerrero, Mexico. HPV detection was performed by PCR. AgNORs were identified by argentic impregnation. One hundred cells per slide were counted and classified according to the polymorphism of AgNORs dots; typical (spherical and atypical (large, kidney-shaped and clustered. RESULTS: A total of 100% of the cases were positive for HPV infection. Nine different high-risk HPV genotypes were found, type16 was the most common (48.6%. The AgNORs showed a significant decrease in spherical shape according to neoplastic development. The three atypical shapes showed a significant increase in SIL and SCC (p-trendOBJETIVO: Evaluar la relación entre los polimorfismos de AgNORs con las lesiones intraepiteliales escamosas (LIE y carcinoma de células escamosas (CCE. MATERIAL Y MÉTODOS: Se estudiaron sesenta mujeres del estado de Guerrero, México. La detección del VPH fue por PCR y los AgNORs por impregnación argéntica; se contaron 100 células y se clasificaron por tipo de polimorfismo de AgNORs: típico (esférico y atípicos (largo, forma de riñón o de racimo. RESULTADOS: El 100% de los casos presentaron infección por VPH, se encontraron nueve genotipos diferentes de VPH de alto riesgo, el 16 fue el más común (48.6%. La forma esférica de los polimorfismos de AgNORs mostró una disminución con el desarrollo neoplásico y las atípicas incrementaron progresivamente con SIL y SCC (p-tendencia<0.001. CONCLUSIONES: Los polimorfismos AgNORs se incrementan progresivamente con el grado de lesión histológica, y pueden ser útiles en el pronóstico de progresión del carcinoma cervical.

  13. Study of the relevance of HPV 16 physical states and the degree of Cervical Lesions%人乳头瘤病毒HPV16整合与宫颈病变程度的研究

    Institute of Scientific and Technical Information of China (English)

    朱义保; 谢彤; 杨亮亮; 万磊

    2015-01-01

    目的:研究HPV 16整合与宫颈病变程度的关系,探讨反应宫颈病变的指标。方法收集感染HPV16的标本,慢性宫颈炎/CINⅠ25例(慢性宫颈炎21例,CINⅠ4例),CINⅡ22例,CIN Ⅲ23例,宫颈癌28例,对不同HPV16质粒浓度的E2和E7基因进行定量PCR分析,制作标准曲线,得出游离型和混合型HPV16 E2/E7的临界值,并对样本进行扩增,根据临界值,分析HPV16整合在不同宫颈病变的分布情况。结果标准曲线制作良好,相关系数均在0.97以上;不同HPV16质粒浓度的E2/E7比值显示,0.77为游离型和混合型HPV16的临界值;根据该临界值,发现慢性宫颈炎/CINⅠ、CINⅡ、CINⅢ、宫颈癌的HPV16的整合率分别为4.00%、9.09%、13.04%、10.71%,统计显示HPV16整合率在不同宫颈病变中没有统计学差异(P>0.05),因混合型HPV16也存在病毒整合,把整合型和混合型HPV16合并,得出HPV16总整合率在慢性宫颈炎/CINⅠ、CINⅡ、CINⅢ、宫颈癌分别为48.00%、59.09%、65.22%、75.00%,分析显示HPV16总整合率在各宫颈病变程度间亦不显著(P>0.05),HPV16总整合率在宫颈病变的分布亦无差异。结论 HPV16整合与宫颈病变程度的关系不明显,病毒整合是宫颈病变的早期事件,不能反应宫颈病变程度的变化。%Objective To study the connection of HPV 16 integration and the degree of Cervical Lesions, and investigate the in-dex of Cervical Lesions. Methods HPV16 infected samples were collected, including 21 chronic cervicitis, 4 CINⅠ, 22 CINⅡ, 23 CINⅢand 28 cervical carcinoma. Standard curves were made by amplifing E2 and E7 gene of HPV16 plasmid with different con-certration by Quantitative PCR, and the cut-off value was obtained. Then samples were detected and the distribution of HPV 16 in-tegration was analyzed in different Cervical Lesions according the cut-off value. Results The standard curves were well made, corre-lation coefficients

  14. Clinical report in treating cervical disc herniation by nucleoplasty of percutaneous radiofrequency abrasion%多靶点射频消融术治疗颈椎间盘突出症临床疗效分析

    Institute of Scientific and Technical Information of China (English)

    卫秀洋; 陈勇忠; 周清碧; 王金星; 陈庆泉

    2013-01-01

    目的 探讨射频消融髓核成形术治疗颈椎间盘突出症的临床疗效.方法 回顾2009 年10月~2010年10月福州总医院476医院应用经皮穿刺多靶点射频消融髓核成形术治疗颈椎间盘突出症168 例.按Macnab 疗效评定标准和视觉模拟评分法(VAS),对比患者手术前后及随访时进行疗效评定.结果 按Macnab 疗效评分标准,术后即刻优76例,良64例,可22例,差6例,优良率为83.33%.本组术前VAS评分为(6.76±0.52)分,术后即刻为(2.15±0.58)分,术后3个月为(2.68±0.56)分,术后1年为(2.95±0.63)分,术后即刻、术后3个月、术后1年评分与术前比较差异有高度统计学意义(P < 0.01).结论 射频消融髓核成形术治疗颈椎病具有操作简单、安全微创、并发症少、起效快及近、中期疗效满意等特点,是治疗轻度颈椎间盘突出症较好的方法.%Objective To observe therapeutic effect of radiofrequency ablation nucleoplasty on cervical disc herniation. Methods From October 2009 to October 2010, 168 patient with cervical disc herniation were analyzed NO.476 Hospital of PLA, who were treated by percutaneous multi-target radiofrequency ablation nucleoplasty treatment. Therapeutic effects were evaluated before and after surgery, 3 month and 12 month by using the average scores of VAS and Mac-nab evaluation standard. Results All cases were followed-up from immediate postoperative to 12 months after operation. According to Macnab efficacy score, the immediate postoperative period were excellent in 76 cases, good in 64 cases, maybe 22 cases and poor in 6 cases, the total good rate of 83.33%. The preoperative VAS score was (6.76±0.52) scores, immediately after was (2.15 ±0.58) scores, 3 months after surgery was (2.68 ±0.56) scores, after an annual was (2.95±0.63) scores. There were significant difference between preoperative surgery and intraoperative immediately after surgery, 3 months after surgery, 1 year after surgery (P < 0

  15. The character and significance of the subtypes of human papillomaviruses in the cervical lesions%宫颈病变中HPV亚型感染特点及意义

    Institute of Scientific and Technical Information of China (English)

    陶琨; 杨静; 杨华; 郭振华; 陈向宇; 孟晓彦; 王春蕾; 唐龙英; 左绪磊

    2011-01-01

    Objective: To investigate the character and significance of the subtypes of human papillomaviruses in the cervical lesions. Methods: The genotypes of the HPVs in the samples of 277 cases were collected and measured by colposcopic biopsy, the HPVs in the cervical cells were analyzed and quantitatively measured by using the Hybrid Capture Ⅱ. Results: The single infection of the HPV in creased as the cervical lesions became more seriously, but the multi - infections decreased. The difference between CIN Ⅰ and CIN Ⅲ and squamous cell carcinoma were statistically significant ( P <0.05 ) . The most common subtypes of the HPVs were type 16, type 58, type 33,type 52, type 31 and type 18. Both the classification of the HPVs and the detective of the Hybrid Capture Ⅱ demonstrated that the infection rate of the HPVs increased as the cervical lesions became seriously, but the difference between the two detective methods was not statistically significant (P > 0.05) . Conclusion: The detectives of the HPV subtypes can be used in scanning the cervical lesions of the high risk population for the early diagnosis and management. The understanding of the distribution of the subtypes of the HPVs will aid the research and development of the vaccines of the human papilloma viruses.%目的:探讨宫颈病变中HPV亚型感染特点及意义.方法:收集277例阴道镜活检病例行HPV基因型测定;相应的宫颈细胞行HPV杂交捕获定量(HCⅡ)检测.结果:随着宫颈病变程度加重,HPV一重感染逐步上升,多重感染逐步下降,CIN Ⅰ组与CINⅢ组、鳞癌组比较差异有统计学意义(P<0.05);HPV亚型感染频度前六位依次为16、58、33、52、31、18;HPV分型与HCⅡ均显示随着宫颈病变程度的加重,HPV感染率上升,但两种方法检测结果差异无统计学意义(P>0.05).结论:HPV亚型检测可以用于高危人群的宫颈病变筛查,可达到早发现、早治疗的目的,并且明确HPV亚型的分布特点,有助于HPV疫苗的开发应用.

  16. 阴道镜检查及活检诊断宫颈病变240例分析%Analysis of colposcopy and biopsy in the diagnosis of cervical lesions in 240 cases

    Institute of Scientific and Technical Information of China (English)

    古丽尼沙·特海

    2015-01-01

    Objective:To explore the diagnosis value of colposcopy and biopsy in the diagnosis of cervical lesions.Methods:240 patients with cervical lesions were selected.They were given colposcopy and biopsy.Results:The colposcopy and vaginal biopsy were compared with pathological diagnosis,and the diagnose accordance rate of colposcopy was higher than that of vaginal biopsy. Conclusion:Colposcopy is better than vaginal biopsy,so colposcopy can be used as an important method of cervical disease screening.%目的:探讨阴道镜检查及活检诊断宫颈病变的诊断价值。方法:收治宫颈病变患者240例,均采用阴道镜检查及活检。结果:阴道镜检查、阴道活检与病理诊断情况比较,阴道镜检查的诊断符合率高于阴道活检。结论:阴道镜检查优于阴道活检,阴道镜检查可以作为筛查宫颈疾病的重要方法。

  17. Human papilloma virus infection in patients with cervical lesions and distribution of its subtypes%宫颈病变患者人乳头状瘤病毒感染状况及其亚型分布的研究

    Institute of Scientific and Technical Information of China (English)

    方美玉; 屠巧凤; 沈宝珠

    2012-01-01

    OBJECTIVE To investigate human papilloma virus (HPV) infections and the distribution of its subtypes in patients with cervical lesions and put forward counter measures for prevention and treatment of the disease. METHODS Cervical exfoliated cells collected from a total of 600 patients with cervical lesions were detected with Flow-through hybridization and gene chip technology, and followed by a complete evaluation of the HPV infection in related to different subtypes of cervical lesions. RESULTS The total HPV infection rate reached 78. 0%. The high-risk and low-risk HPV infection rates of patients with cervicitis/ condyloma acuminatum were 73. 1% and 18. 1%, respectively. The difference was statistically significant (P<0. 05). The high-risk and low-risk HPV infection rates of patients with cervical intraepithelial neoplasia (CIN) were 82. 6% and 7. 9%, respectively, the difference was statistically significant (P<0. 01). The high-risk and low-risk HPV infection rates of patients with cervical cancer were 96. 0% and 0, respectively, the difference was statistically significant (P<0. 01). The high-risk HPV infection rate increased with the degree of exacerbating of cervical lesions. The rate of single-type HPV infection was significantly higher than that of multi-type HPV infection in all kinds of cervical lesions (P<0. 01), while the rate of multi-type HPV infection increased with the increasing severity of cervical lesions. Different subtypes of HPV distributed differently in cervix lesions. CONCLUSION Detection of HPV subtypes is helpful for screening of the cervical lesions and diagnosis of the condition. For those patients infected especially with high-risk HPV, it is critical to conduct the early diagnosis, timely treatment and close follow-up to block the persistent HPV infection and prevent cervical cancer.%目的 探讨宫颈病变患者人乳头状瘤病毒(HPV)的感染状态及其亚型分布,提出预防治疗疾病的干预措施.方法 采用导流

  18. Conduit Coating Abrasion Testing

    Science.gov (United States)

    Sullivan, Mary K.

    2013-01-01

    During my summer internship at NASA I have been working alongside the team members of the RESTORE project. Engineers working on the RESTORE project are creating ·a device that can go into space and service satellites that no longer work due to gas shortage or other technical difficulties. In order to complete the task of refueling the satellite a hose needs to be used and covered with a material that can withstand effects of space. The conduit coating abrasion test will help the researchers figure out what type of thermal coating to use on the hose that will be refueling the satellites. The objective of the project is to determine whether or not the conduit coating will withstand the effects of space. For the RESTORE project I will help with various aspects of the testing that needed to be done in order to determine which type of conduit should be used for refueling the satellite. During my time on the project I will be assisting with wiring a relay board that connected to the test set up by soldering, configuring wires and testing for continuity. Prior to the testing I will work on creating the testing site and help write the procedure for the test. The testing will take place over a span of two weeks and lead to an informative conclusion. Working alongside various RESTORE team members I will assist with the project's documentation and records. All in all, throughout my internship at NASA I hope to learn a number of valuable skills and be a part of a hard working team of engineers.

  19. RELATIONSHIP BETWEEN ETIOLOGY OF CONTACT VAGINAL BLEEDING AND CERVICAL LESIONS%接触性阴道出血的病因分析及与宫颈病变的关系

    Institute of Scientific and Technical Information of China (English)

    李巧云; 刘秀荣; 李桂荣; 冯玉珍

    2015-01-01

    目的:通过对接触性阴道出血的病因进行分析,研究其与宫颈病变的关系。方法选择2012年10月—2014年10月在该院接受治疗的150例妇女作为研究对象,给予常规的妇科检查与细胞学检查。结果妇科检查结果为宫颈糜烂(62.67%)所占的比例明显高于检查的其他结果如宫颈息肉(19.33%)、阴道炎(7.33%)、尖锐湿疣(3.33%)、宫颈癌(2.67%)等,宫颈涂片细胞学检查结果为巴氏Ⅱ级所占的比例(61.33%)明显高于巴氏Ⅰ级(29.33%)、Ⅲ级(5.33%)、Ⅳ级(2.67%)与Ⅴ级(1.33%),Ⅲ~Ⅴ级患者阴道镜活检结果宫颈上皮内瘤样变Ⅰ级(35.71%)明显高于宫颈原癌(14.29%)、宫颈鳞癌(7.14%)、腺癌1例(7.14%)及宫颈上皮内瘤样变Ⅱ级(21.43%)与Ⅲ级(14.29%),差异具有统计学意义(P <0.05)。结论接触性出血病因错综复杂,多数检查结果为良性,但部分为宫颈癌的早期病兆,针对接触性出血患者建议早期检查,避免宫颈病变,减少宫颈癌的发生。%Objective To study the correlation between etiology of vaginal contact bleeding and cervical le-sions by an etiological analysis.Methods A total of 150 cases of women who achieved treatment from Oc-tober 2012 to October 2014 in author's hospital were selected as the research subjects,they were given the gynecological examination and routine cytologic examination.Results In these subjects,the gynecological examination results showed that cervical erosion (62.67%)proportion was obvious higher than that of oth-er examination results such as cervical polyp (19.33%),vaginitis (7.33%),condyloma acuminatum (3.33%),cervical cancer (2.67%).Papanicolaou grade Ⅱ accounted for the proportion of (61.33%)was obviously higher than the PAP Ⅰ grade (29.33%),Ⅲ (5.33%),Ⅳ(2.67%)and Ⅴ (1.33%).Ⅲ ~ Ⅴlevel colposcopy biopsy results in patients with cervical intraepithelial neoplasia grade I (35.71%)was sig-nificantly higher than that of primary cervical cancer (14

  20. Cervical dysplasia

    Science.gov (United States)

    ... by your provider. Make sure to get the HPV vaccine when it is offered to you. This vaccine prevents many cervical cancers. ... Early diagnosis and prompt treatment cures most cases of cervical ... severe cervical dysplasia may change into cervical cancer.

  1. 宫颈薄层液基细胞学检查联合高危型HPV检测在宫颈病变筛查中的临床应用%CLINICAL APPLICATION OF CERVICAL THIN PREP CYTOLOGIC-TEST AND HIGH-RISK HPV TYPE TESTING IN SCREENING OF CERVICAL LESIONS

    Institute of Scientific and Technical Information of China (English)

    朱华洁

    2012-01-01

    目的 研究宫颈薄层液基细胞学检查(thin prep cytologic test,TCT)联合高危型人乳头瘤病毒(human papilloma virus,HPV)检测在子宫颈病变筛查中的诊断价值.方法对2010年1-12月行宫颈病变筛查的患者680例,进行TCT和高危型HPV检测,同时行阴道镜下组织活检.结果 680例患者中宫颈TCT检查结果异常180例,对宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)诊断的敏感性为85.57%,特异性为97.12%,漏诊率为4.12%.TCT与HPV联合检查,诊断敏感性为89.69%,特异性为85.80%,漏诊率1.62%.HPV感染率为26.91%,随着细胞学和组织学诊断级别的升高,HPV感染率不断上升.结论 TCT与高危型HPV联合检测是筛查宫颈癌及癌前病变的有效方法,可防止漏诊,有利于提高宫颈上皮内瘤变的诊断率.%Objective To explore the diagnostic value of cervical thin prep cytologic test ( TCT ) combined with high - risk human papilloma virus ( HPV ) detection in the screening of cervical lesions. Methods A total of 680 patients for cervical lesions screeing underwent TCT and high - risk HPV type detection in gynecology outpatient from January 2010 to December 2010, meanwhile, biopsy was performed under colposcopy. Results Among 680 cases,the result of cervical TCT was abnormal in 180 cases. The sensitivity, specificity and the rate of missed diagnosis of TCT for the diagnosis of cervical intraepithelial neoplasia ( CIN ) was 85.57% , 97. 12% and 4. 12% , respectively, while thoses of combination of TCT and HPV was 89. 69% ,85. 80% and 1. 62%. The infection rate of HPV was 26. 91%. With the increase of cytological and histological grading, the infection rate of HPV increased. Conclusion TCT combined with high risk HPV detection is an effective method in screening cervical cancer and precancerous lesions. It helps to prevent missed diagnosis and improve the diagnosis rate of CIN.

  2. Complete Genome Sequences of Eight Human Papillomavirus Type 16 Asian American and European Variant Isolates from Cervical Biopsies and Lesions in Indian Women

    Science.gov (United States)

    Mandal, Paramita; Sen, Shrinka; Bhattacharya, Amrapali; Roy Chowdhury, Rahul; Mondal, Nidhu Ranjan

    2016-01-01

    Human papillomavirus type 16 (HPV16), a member of the Papillomaviridae family, is the primary etiological agent of cervical cancer. Here, we report the complete genome sequences of four HPV16 Asian American variants and four European variants, isolated from cervical biopsies and scrapings in India. PMID:27198009

  3. Complete Genome Sequences of Eight Human Papillomavirus Type 16 Asian American and European Variant Isolates from Cervical Biopsies and Lesions in Indian Women

    OpenAIRE

    Mandal, Paramita; Bhattacharjee, Bornali; Sen, Shrinka; Bhattacharya, Amrapali; Roy Chowdhury, Rahul; Mondal, Nidhu Ranjan; Sengupta, Sharmila

    2016-01-01

    Human papillomavirus type 16 (HPV16), a member of the Papillomaviridae family, is the primary etiological agent of cervical cancer. Here, we report the complete genome sequences of four HPV16 Asian American variants and four European variants, isolated from cervical biopsies and scrapings in India.

  4. Complete Genome Sequences of Eight Human Papillomavirus Type 16 Asian American and European Variant Isolates from Cervical Biopsies and Lesions in Indian Women.

    Science.gov (United States)

    Mandal, Paramita; Bhattacharjee, Bornali; Sen, Shrinka; Bhattacharya, Amrapali; Roy Chowdhury, Rahul; Mondal, Nidhu Ranjan; Sengupta, Sharmila

    2016-01-01

    Human papillomavirus type 16 (HPV16), a member of the Papillomaviridae family, is the primary etiological agent of cervical cancer. Here, we report the complete genome sequences of four HPV16 Asian American variants and four European variants, isolated from cervical biopsies and scrapings in India. PMID:27198009

  5. Study on detection and treatment of cervical lesions in 10 212 women in Yingcheng%应城市10212例妇女宫颈病变检测及治疗的研究

    Institute of Scientific and Technical Information of China (English)

    刘永桥; 李艳; 杨丽

    2012-01-01

    Objective; To detect, diagnose, and evaluate cervical lesions, especially the degree and state of cervical precancerous lesion by liquid - based thin layer cytological test ( TCT) combined with biopsy, provide an accurate reference for treating cervical lesions reasonably in clinic, observe the curative effect. Methods; A total of 10 212 women were detected by TCT for successive three years, including 457 female workers from the hospital. Cytological diagnosis was made according to TBS criteria. Partial women with positive TCT results received cervical biopsy. Results; Among 10 212 women, 391 women were found with positive cytological results (above ASC - US) , the positive rate was 3. 83% , including 296 women with ASC - US (2. 90% ) , 85 women with ASC - H ( 0. 84% ) , 5 women with LSIU (0. 05% ) , 3 women with HSIL (0. 03% ) , one woman with SCC (0. 01% ) , and one woman with AGC (0. 01% ) . The positive rate of cytologieal screening in 457 female workers in the hospital for successive three years was 3.42% , 0. 88% , and 0.00% , respectively. Conclusion: TCT has distinct advantages for cervical lesions screening and curative effect observation after gynecological treatment , TCT combined with cervical biopsy and treatment can reduce the occurrence and development of cervical lesions, which has important clinical application value.%目的:采用液基薄层细胞制片技术(TCT)结合组织活检术检测、诊断和评价宫颈病变,特别是癌前病变的程度及所处的状态,为临床合理治疗宫颈病变提供准确依据,观察治疗后的效果.方法:运用TCT连续3年检测了10 212例妇女,包括457例应城市人民医院女职工.细胞学诊断按TBS标准.对TCT部分阳性者进行宫颈活检.结果:10 212例妇女中,细胞学阳性(ASC-UC以上)391例,阳性率为3.83%,其中ASC-UC 296例(2.90%)、ASC-H 85例(0.84%)、LSIL 5例(0.05%)、HSIL 3例(0.03%)、SCC 1例(0.01%)、AGC 1例(0.01%).应城市人民医院457

  6. 液基细胞学及HPV分型检测在宫颈病变筛查中的应用%Thinprep Cytology Test and HPV Genotyping in Screening of Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    焦凤娟; 韩洁

    2013-01-01

    Objective: To investigate the clinical significance of thinprep cytology test (TCT) and HPV genotyping in cervical le-sions. Methods: TCT and HPV genotyping were done for 390 cases of cervical lesions, and did the biopsy for who was ≥ASCUS. Results: In the 390 cases, the positive rates of TCT and HPV were 26.41% and 28.72%; the rate of high-risk type of HPV in 103 cas-es of TCT-positive patients was 63.11%, and the biopsy showed that there were inflammation 46 cases, CIN I 24 cases, CIN II 12 cases, CIN III 18 cases and cervical cancer 3 cases. Conclusion: TCT and HPV genotyping can improve the detection rate of cervical cancer and precancerous lesions, it has important significance in early diagnosis and prevention for cervical cancer.%  目的:探讨液基细胞学(TCT)联合HPV 分型检测在宫颈病变筛查中的临床意义.方法:对390例宫颈病变患者行TCT 检查和 HPV 检测,对≥ASCUS 患者行组织活检.结果:390例患者中 TCT 和 HPV 阳性率分别为26.41%、28.72%;103例TCT阳性患者中高危型HPV阳性率为63.11%,组织活检结果为炎症46例、CIN I 24例、CIN II 12例、CIN III 18例和宫颈癌3例.结论:TCT联合HPV检测可提高对宫颈癌及癌前病变的检出率,对宫颈癌的早期诊断和防治有重要临床意义.

  7. The expression of P16 INK4A and MIB-1 in cervical cancer and cervical cancer lesion before%P16INK4A与MIB-1在宫颈癌及宫颈癌前病变中的表达

    Institute of Scientific and Technical Information of China (English)

    王志新; 肖明明

    2011-01-01

    目的:探讨P16 INK4A与MIB-1在宫颈癌及宫颈癌前病变中的表达情况.方法:采用免疫组织化学方法检测P16 INK4A与MIB-1在63例宫颈组织及38例宫颈涂片中的表达.结果:P16在正常宫颈上皮中均无表达.随着宫颈病变级别的增高,其阳性表达率逐渐增大,其中,宫颈癌中P16的表达增强明显.MIB-1随着宫颈病变级别的增高,表达增强,其中,宫颈癌中MIB-1的表达增强明显.在宫颈病变组织中,随着P16阳性表达率的增大,MIB-1表达也相应增高,具有相关性.结论:宫颈上皮内病变中P16及MIB-1过表达,提示两者可以作为宫颈病变的诊断标志物.在宫颈病变及宫颈癌的确切诊断中,P16及MIB-1可以作为诊断依据之一.%Objective: To evaluate the expression of P16 INK4A and MIB-1 in cervical cancer and cervical cancer lesion before.Methods: Immunohistochemistry methods were used to detect the expression of P16 INK4A and MIB-1 in 63 cases of cervical tissue and 38 cases of cerical smear.Results: No positive staining of P16 was observed in the normal cervical epithelium.With increasing severity of cervical intraepithelial neoplasias (CIN), the P16 expression increased progressively, significant up-regulation of P16 was observed in carcinoma cervix.MIB-1 was observed with increasing severity of CIN, and significant overexpression of MIB-1 was observed in carcinoma cervix.The expression of MIB-1 was increased with consistently increasing of P16, there was a correlation between P16 and MIB-1 in cervical lesions organization.Conclusion: The overexpression of P16 and MIB-1 demonstrates that they can be used as a diagnostic marker for cervical lesions.Therefore, in the exact diagnosis of cervical lesions and cervical cancer, P16 and MIB-1 markers can be used as one of the diagnostic bases.

  8. Prevalence and Distribution of High-Risk Genotypes of HPV in Women with Severe Cervical Lesions in Madrid, Spain: Importance of Detecting Genotype 16 and Other High-Risk Genotypes.

    Science.gov (United States)

    Mateos Lindemann, Maria Luisa; Sánchez Calvo, Juan Manuel; Chacón de Antonio, Jesús; Sanz, Itziar; Diaz, Esperanza; Rubio, Maria Dolores; de la Morena, Maria Luisa

    2011-01-01

    Background. Persistent infection with high-risk human papillomavirus (HR-HPV) has been demonstrated to be the necessary causal factor for developing cervical cancer. To know the most prevalent HR-HPV in different geographical areas is important to design diagnostic tests and implementation of vaccines. Objectives. The goal of this study is to evaluate the prevalence of HR-HPV in a total of 1001 patients, 198 with normal cytology results, 498 with low-grade squamous intraepithelial lesion (LSIL), and 205 with high-grade squamous intraepithelial lesion (HSIL) who attended our gynaecology department for opportunistic screening of HPV infection. Study design. Cervical samples were taken in a PreservCyt vial (Cytyc Corporation, Boxborough, MA). Hybrid capture assay was carried out following the manufacturer's instructions (Digene Corp., Gaithersburg, MD). All samples were further studied with polymerase chain reaction (PCR) (Linear Array HPV Genotyping Test, Roche Diagnostics, Mannheim, Germany). Results. Genotype 16 was the most prevalent HR-HPV in the three groups, 17.8% in the patients with normal cytology results, 22.3% in the LSIL group, and 60% in the HSIL group. Genotype 18 had a very low prevalence in all groups. Other HR-HPV genotypes such as genotype 31, genotype 58 and genotype 52 were found in significant numbers in HSIL patients. Discussion. Our data show that genotypes 16, 31, 58, and 52 are the most prevalent HR-HPV in cervical samples with severe intraepithelial lesion in Spain. There may be some geographical variation in prevalence of carcinogenic types, and it must be considered for designing diagnostic tests and vaccine.

  9. Value of Cervical Fluid Based Cytology and Vaginal Examination in Early Screening for Cervical Lesions%宫颈病变早期筛查中宫颈液基细胞学和阴道镜检查的应用价值探析

    Institute of Scientific and Technical Information of China (English)

    张书霞

    2015-01-01

    目的 研究和探析宫颈病变早期筛查中宫颈液基细胞学和阴道镜检查的应用价值. 方法 收集2013年1月-2014年12月到该院妇科门诊行宫颈检查的患者共1 037例,所有患者均接受宫颈液基细胞学检测,对检查结果呈阳性者进行阴道镜检查. 结果 37例(3.6%)患者宫颈液基细胞学检测呈阳性,经过阴道镜检查后,23例显示阳性,其中炎症12例(32.4%),宫颈上皮内瘤样病变Ⅰ期14例(37.8%), 宫颈上皮内瘤样病变Ⅱ~Ⅲ6例(16.2%). 阴道镜下的活检组织病理学检查结果与宫颈液基细胞学的检查结果的差异有统计学意义(P<0.05).结论 在宫颈病变早期筛查中,宫颈液基细胞学和阴道镜活组织病理检查联合使用,有助于尽早发现宫颈病变,值得推广应用.%Objective To study the application value of cervical fluid based cytology and vaginal examination in early screening for cervical lesions. Methods 1037 gynecological outpatients who underwent inspection of the cervix in the hospital between January 2013 and December 2014 were included. All of them received cervical fluid based cytology, and for those with positive results vaginoscopy was carried out. Results Out of 37 patients (3.6%) who showed positive result from cervical fluid based cytology, 23 showed positive result after vaginoscopy, including 12 (32.4%) cases of f inflammation and 14(37.8%) cases of stage I cervical in-traepithelial neoplasia, and 6 (16.2%) cases of stage II-III cervical intraepithelial neoplasia. Therefore, there was statistically sig-nificant difference in the outcomes between vaginoscopy and cervical fluid based cytology, P<0.05. Conclusion In the early screening of cervical lesions, the cervical fluid based cytology combined with pathological examination of biopsy under vaginoscope is beneficial to the early detection of cervical lesions, therefore it is worthy of popularization and application.

  10. Avaliação da conduta conservadora na lesão intraepitelial cervical de alto grau Evaluación de la conducta conservadora en la lesión intraepitelial cervical de alto grado Evaluation of conservative management of high-grade cervical squamous intraepithelial lesion

    Directory of Open Access Journals (Sweden)

    Nelson Shozo Uchimura

    2012-06-01

    transversal y retrospectivo realizado con 509 mujeres (15-76 años atendidas en el período de 1996 a 2006, con colpocitología oncótica alterada, en un servicio público de referencia en Maringá, Sur de Brasil. Los datos fueron colectados de los prontuarios médicos y se estudiaron las variables diagnóstico definitivo, tipos de tratamiento, ocurrencia de la lesión y reincidencias y se analizaron por medio de pruebas de asociación de chi-cuadrado de Pearson y exacto de Fisher. RESULTADOS: La lesión intraepitelial cervical de alto grado ocurrió en 168 casos; de estos, 31 mujeres se sometieron a la amputación cónica, 104 a cirugías de alta frecuencia, nueve a histerectomías y 24 recibieron conducta conservadora. Entre las mujeres con lesión de alto grado y tratadas de forma conservadora, ocho (33,3% reincidieron, mientras que de las sometidas a la conducta no conservadora, diez (6,9% reincidieron, siendo esta diferencia estadísticamente significativa (p=0,0009, RP=4,8 (IC95% 2,11;10,93. En aquellas que hicieron seguimiento clínico-citológico, tres (30,0% y, entre las cauterizadas, cinco (35,7% reincidieron en el plazo de tres años, sin diferencia significativa (p=0,5611. La reincidencia por debajo y por encima de los 30 años ocurrió, respectivamente, en siete (13,8% y 11 (12,2% mujeres (p=0,9955. CONCLUSIONES: La edad de la mujer no influencia el pronóstico de la reincidencia. El tratamiento conservador debe ser indicado como conducta de excepción, dada la alta tasa de reincidencia, y el seguimiento debe ser riguroso, con acompañamiento citológico y colposcópico por inclusive tres años, período en el que ocurre la mayoría de las reincidencias.OBJECTIVE: To assess the association between conservative management of high-grade cervical squamous intraepithelial lesions and recurrence rates and age groups. METHODS: Cross-sectional, retrospective, analytical observational study of 509 women (aged 15 to 76 with abnormal Pap smears attending a public reference

  11. CD28-, CD45RAnull/dim and natural killer-like CD8+ T cells are increased in peripheral blood of women with low-grade cervical lesions

    OpenAIRE

    Pita-Lopez, Maria Luisa; Ortiz-Lazareno, Pablo Cesar; Navarro-Meza, Monica; Santoyo-Telles, Felipe; Peralta-Zaragoza, Oscar

    2014-01-01

    Background In response to antigen naive CD8+, T cells differentiate into effector cells, which express Natural killer (NK) receptors, lose CD28 expression, and die by apoptosis. However, in smaller quantities, the cells are retained for subsequent exposure to the same antigen. Knowledge is limited regarding whether the percentages of CD28-, Effector memory (EMRAnull/dim), and the CD16+/CD56 + CD8+ T cells of women with low-grade cervical lesions are altered at a systemic level. Methods We enr...

  12. Augmented serum level of major histocompatibility complex class I-related chain A (MICA) protein and reduced NKG2D expression on NK and T cells in patients with cervical cancer and precursor lesions

    International Nuclear Information System (INIS)

    Cervical cancer is the second most common cancer in women worldwide. NK and cytotoxic T cells play an important role in the elimination of virus-infected and tumor cells through NKG2D activating receptors, which can promote the lysis of target cells by binding to the major histocompatibility complex class I-related chain A (MICA) proteins. Increased serum levels of MICA have been found in patients with epithelial tumors. The aim of this study was to compare the levels of soluble MICA (sMICA) and NKG2D-expressing NK and T cells in blood samples from patients with cervical cancer or precursor lesions with those from healthy donors. Peripheral blood with or without heparin was collected to obtain mononuclear cells or sera, respectively. Serum sMICA levels were measured by ELISA and NKG2D-expressing immune cells were analyzed by flow cytometry. Also, a correlation analysis was performed to associate sMICA levels with either NKG2D expression or with the stage of the lesion. Significant amounts of sMICA were detected in sera from nearly all patients. We found a decrease in the number of NKG2D-expressing NK and T cells in both cervical cancer and lesion groups when compared to healthy donors. Pearson analysis showed a negative correlation between sMICA and NKG2D-expressing T cells; however, we did not find a significant correlation when the analysis was applied to sMICA and NKG2D expression on NK cells. Our results show for the first time that high sMICA levels are found in sera from patients with both cervical cancer and precursor lesions when compared with healthy donors. We also observed a diminution in the number of NKG2D-expressing NK and T cells in the patient samples; however, a significant negative correlation between sMICA and NKG2D expression was only seen in T cells

  13. CCR2-V64I polymorphism is associated with increased risk of cervical cancer but not with HPV infection or pre-cancerous lesions in African women

    International Nuclear Information System (INIS)

    Cervical cancer, caused by specific oncogenic types of human papillomavirus (HPV), is the second most common cancer in women worldwide. A large number of young sexually active women get infected by HPV but only a small fraction of them have persistent infection and develop cervical cancer pointing to co- factors including host genetics that might play a role in outcome of the HPV infection. This study investigated the role of CCR2-V64I polymorphism in cervical cancer, pre-cancers and HPV infection in South African women resident in Western Cape. CCR2-V64I polymorphism has been previously reported to influence the progression to cervical cancer in some populations and has also been associated with decreased progression from HIV infection to AIDS. Genotyping for CCR2-V64I was done by PCR-SSP in a case-control study of 446 women (106 black African and 340 mixed-ancestry) with histologically confirmed invasive cervical cancer and 1432 controls (322 black African and 1110 mixed-ancestry) group-matched (1:3) by age, ethnicity and domicile status. In the control women HPV was detected using the Digene Hybrid Capture II test and cervical disease was detected by cervical cytology. The CCR2-64I variant was significantly associated with cervical cancer when cases were compared to the control group (P = 0.001). Further analysis comparing selected groups within the controls showed that individuals with abnormal cytology and high grade squamous intraepitleial neoplasia (HSIL) did not have this association when compared to women with normal cytology. HPV infection also showed no association with CCR2-64I variant. Comparing SIL positive controls with the cases showed a significant association of CCR2-64I variant (P = 0.001) with cervical cancer. This is the first study of the role of CCR2-V64I polymorphism in cervical cancer in an African population. Our results show that CCR2-64I variant is associated with the risk of cervical cancer but does not affect the susceptibility to HPV

  14. Triage of women with minor cervical lesions: data suggesting a "test and treat" approach for HPV E6/E7 mRNA testing.

    Directory of Open Access Journals (Sweden)

    Sveinung Wergeland Sørbye

    Full Text Available BACKGROUND: Human papillomavirus (HPV testing is included in the cervical cancer screening program in the triage of women with equivocal (ASC-US or low-grade (LSIL cytological lesions. These women have an increased risk for developing high grade dysplasia and cancer (CIN2+ compared to women with normal cytology. However, in order to avoid unnecessary follow-up, as well as overtreatment, a high positive predictive value (PPV of the triage test is important. METHODOLOGY/PRINCIPAL FINDINGS: The HPV test PreTect HPV-Proofer, detecting E6/E7 mRNA from the HPV types 16, 18, 31, 33 and 45, is used as triage test together with repeat cytology. PPV data for HPV E6/E7 mRNA testing during the period from January 2006 up to June 2009 are reported. In total, 406 of 2099 women (19.3% had a positive HPV test result. Of the women with a positive test result and with a histological diagnosis (n = 347, 243 women had histological high-grade dysplasia or cancer (CIN2+, giving a PPV of 70.0% (95% confidence interval [CI], 65.2%-74.8%. For HPV 16 or HPV 33 positive women above 40 years of age, the PPV was 83.7% (95% CI, 73.3%-94.0% and 84.6% (95% CI, 65.0%-100.0% respectively. The PPV of test positive women with HSIL cytology was 94.2% (95% CI, 88.7%-99.7%. CONCLUSIONS: When the result in triage is HPV mRNA positive, our data suggest direct treatment for women above 40 years of age or for women with a concurrent cytological HSIL diagnosis, contributing to better clinical safety for these women. In addition, by decreasing the time to treatment, thereby reducing the number of recalls, the patient management algorithm will be considerably improved, in turn reducing follow-up costs as well as unnecessary psychological stress among patients.

  15. HC2-HPV-DNA 检测在宫颈癌病变中的临床分析%Clinical Analysis of HC2-HPV-DNA Testing in Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    季冰; 何浪驰; 郭芝亮; 施全; 黄子健; 黄峥; 黄柳

    2015-01-01

    Objective To observe hybrid capture 2 high-risk human papilloma virus DNA(HC2-HPV-DNA) in cervical lesion screening and accuracy .Methods 110 cases of patients with suspected symptoms of cervical lesion were selected as the research object,and respectively received liquid-based thinlayer cytology test(LCT)inspection,HC2-HPV-DNA testing and cervi-cal tissues pathology biopsy .Positive rate,sensitivity,specificity,and predictive value of LCT,HC2-HPV-DNA testing and the joint inspection were analyzed.Results HC2-HPV-DNA positive rate increased with the increase of cervical lesion severity ,there had statistical difference(P <0.05),sensitivity,specificity,and positive predictive value of HC2-HPV-DNA with LCT joint detection were 78.1%,40% and 95.3%,respectively,the sensitivity significantly increased than single detection (P <0.05).Conclusion HC2-HPV-DNA is a good reference indicator in cervical lesion screening ,the accuracy increases with the increase of the degree of pathological changes,HC2-HPV-DNA and LCT joint detection can improve the accuracy of the diagnosis of cervical cancer and precancerous lesions.%目的:观察分析采用2代杂交捕获法检测高危人乳头状瘤病毒 DNA(HC2-HPV-DNA)对宫颈癌病变筛查中的应用及准确性。方法选取110例疑似宫颈癌患者为研究对象,分别进行宫颈液基薄层细胞学(LCT)检查、HC2-HPV-DNA 检测及宫颈组织病理活检,观察分析 LCT 检查、HC2-HPV-DNA 检测和二者联合检查阳性率、敏感性、特异性及预测值情况。结果随着宫颈病变严重程度的增加,HC2-HPV-DNA 的阳性率升高,差异有统计学义意(P <0.05), HC2-HPV-DNA 与 LCT 联合检测敏感性、特异性、阳性预测值分别为78.1%、40.0%、95.3%,敏感性较单独检测显著提高(P <0.05)。结论HC2-HPV-DNA 是宫颈癌病变筛选的良好参照指标,随病变程度的增加,

  16. PROGRESS IN THERMO-ABRASIVE BLASTING SYSTEMS

    OpenAIRE

    I.A. Gorlach

    2012-01-01

    ENGLISH ABSTRACT: Quality of surface preparation of components and structures for further painting and/or coating is important in many fields of engineering. One of the most widely used methods of surface preparation is abrasive blasting. In the last few years, a new method for surface preparation has evolved, namely thermo-abrasive blasting. This technique utilises a high enthalpy thermal jet, generated by the thermo-abrasive blasting gun, to propel abrasive particles. Thermo-abrasi...

  17. 30 CFR 72.610 - Abrasive blasting.

    Science.gov (United States)

    2010-07-01

    ... respirators approved for abrasive blasting by NIOSH under 42 CFR part 84, or the operation shall be performed... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Abrasive blasting. 72.610 Section 72.610... HEALTH STANDARDS FOR COAL MINES Miscellaneous § 72.610 Abrasive blasting. (a) Surface and...

  18. Lung scintigraphy evaluation in workers exposed to abrasive dusts

    International Nuclear Information System (INIS)

    The production process of abrasives use aluminium, or silicon carbide a synthetic material with a hardness only slightly less than that of a diamond. It is popularly known as carborundum since it was first manufactured as an abrasive in 1891, produced by the fusion of high grade silica and petroleum coke with sawdust. For many years silicon carbide was thought not to give rise to pulmonary lesions. Recently several researchers suggested the existence of a carborundum pneumoconiosis. The aim of this study was to evaluate the role of the pulmonary clearance of 99m Technetium chelated to diethylene-triamine penta-acetate (99m Tc DTPA), and 67 Gallium lung scanning in workers exposed to abrasive dusts. Thirty seven subjects, 13 smokers and 24 nonsmokers and ex smokers were studied. In 32 (86,48%) 67 Gallium lung scanning was positive including 13 (40,62%) retired workers. We conclude that non smoking workers of abrasives plants have a pulmonary alveolar epithelial permeability disturbance similar as observed in smoking workers and smoking controls. Most workers, ex-workers of these industries and in patients with carborundum pneumoconiosis there is an evidence of pulmonary inflammation measured with abnormal 67 Gallium lung scan. (author)

  19. The value of TCT and HPV-DNA detection in screening of cervical lesions%TCT和HR-HPV DNA检测对宫颈病变筛查的价值

    Institute of Scientific and Technical Information of China (English)

    王琼; 刘勇; 徐子顺; 吴易红

    2015-01-01

    Objective:To investigate the value of thinprep cytology test (TCT) and high-risk human papilloma virus DNA (HR-HPV DNA) detection in the screening of cervical lesions so as to provide a theoretical basis for the government to formulate the vaccine prevention policy. Methods:Two hundred and forty-three women diagnosed or examined in our hospital were selected, to whom TCT and the detection of HR-HPV DNA were performed and their results were analyzed. Results:The speciifcity, positive predictive value and diagnosis rate were obviously higher by the combined detection of TCT and HR-HPV DNA than by single detection of HR-HPV DNA and their differences had statistical significance (P<0.05). Conclusion:The combined detection of TCT and HR-HPV DNA can improve the diagnosis level of cervical lesions and early cervical cancer, reduce the rate of misdiagnosis and has important signiifcance for the early screening of cervical lesions.%目的:探讨液基薄层细胞学检测(TCT)和高危型人乳头瘤状病毒DNA(HR-HPV DNA)检测对宫颈病变筛查的价值,为政府制定疫苗的预防政策提供理论依据。方法:选取在我院就诊或体检的妇女243例,均行TCT、HR-HPV DNA检测,分析其检测结果。结果:TCT+HR-HPV DNA联合检测的特异性、阳性预测值和诊断符合率均明显高于HR-HPV DNA单项检测,差异具有统计学意义(P<0.05)。结论:TCT和HR-HPV DNA联合检测可提高宫颈病变及早期宫颈癌的诊断水平,降低漏诊率,对宫颈病变的早期筛查具有重要意义。

  20. Clinical study on influence of human papillomavirus infection on cervical lesions%人乳头状瘤病毒感染对宫颈病变影响的临床研究

    Institute of Scientific and Technical Information of China (English)

    赵颖辉; 梁贤; 李慧; 高凤萍

    2014-01-01

    目的:研究人乳头状瘤病毒单一感染与多重感染对宫颈病变的影响,以期为临床诊治宫颈病变提供参考依据。方法选取2012年1月-2013年3月入院诊治并应用导流杂交人乳头状瘤病毒分型检测技术确诊的人乳头状瘤病毒感染患者120例,回顾性分析其临床资料,分为人乳头状瘤病毒单一感染组73例与多重感染组47例,观察并比较两组感染对宫颈病变的影响,采用SPSS16.0进行统计分析。结果 HPV单一感染与多重感染高危型亚型分布均以16型为主,分别占35.62%、25.53%,各型分布差异有统计学意义( P<0.05),细胞学结果,诊断高度鳞状上皮内病变单一感染组与多重感染组分别占23.29%与8.51%,差异有统计学意义( P<0.05);病理学结果,单一感染组与多重感染组在诊断患者正常、CIN病变病理学结果差异有统计学意义( P<0.05)。结论单一高危人乳头状瘤病毒亚型对宫颈病变的严重程度有一定的诊断意义;多重人乳头状瘤病毒亚型感染多变现象为低级别宫颈病变。%OBJECTIVE To study the influence of single and multiple infections with human papillomavirus on cervi‐cal lesions ,in order to provide a reference for the clinical diagnosis of cervical lesions .METHODS Totally 120 patients who were admitted to hospital during Jan .2012 to Mar .2013 and confirmed with human papillomavirus infection by flow‐through hybridization detection of human papillomavirus genotyping were enrolled and their clini‐cal data were retrospectively analyzed .The patients were divided into the single infection group (n=73) and the multi‐infection group (n=47) .The two groups were observed and compared for influence of infections on cervical lesions .The software SPSS16 .0 was used for statistical analysis .RESULTS The HPV high‐risk subtype of type 16 was predominant ,accounting for 31 .67% and 25 .53% in

  1. Application of thinprep cytology test and cervical smear combined with colposcope in screening of cervical lesions%液基细胞学、宫颈刮片联合阴道镜在宫颈病变筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    江明珠

    2015-01-01

    目的 探讨液基细胞学(TCT)、宫颈刮片联合阴道镜在宫颈病变筛查中的应用价值. 方法 选取2014年1月~2015年4月来我所妇科门诊行TCT筛查的1726例妇女(门诊组)和在乡镇行宫颈刮片细胞学筛查的26 021例妇女(乡镇组)作为研究对象,两组均行TBS分级诊断,将细胞学阳性者进一步行阴道镜及镜下活组织病理学检查,分析两组不同取材方法的细胞学筛查结果,并观察细胞学阳性者的阴道镜和病理组织学检查结果. 结果 两组细胞学检出阳性率差异有统计学意义(X2=86.56,P0.05). 以病理组织学诊断为金标准,宫颈癌前病变和宫颈癌(CIN+SCC)两者合计,阴道镜诊断符合率为90.14%(128/142), 细胞学诊断符合率为70.73%(87/123). 结论 TCT筛查法优于传统刮片细胞学筛查法,联合应用阴道镜及镜下活检组织病理学检查,可明显提高宫颈癌前病变及宫颈癌的检出.%Objective To discuss the application value of thinprep cytology test(TCT) and cervical smear combined with colposcope in screening of cervical lesions. Methods 1726 cases of female patients (outpatient group) visited outpatient clinic in our hospital from January 2014 to April 2015 and 26 021 cases of rural women (rural group) receiving cervical cancer screening were selected as research object.Two groups were diagnosed by TBS classification,and those with pos-itive cytologic results were further examined by colposcope and histopathological examination.The cytologic screening results in two groups were analyzed by different methods, the colposcope and histopathologic results of patients with positive cytologic results were observed. Results There was significant difference in positive rate of cytologic results be-tween two groups (X2=86.56,P0.05).The gold standard was diagnosed by histopathology.The total of cervical precancerous lesions and cervical cancer (CIN+SCC),colposcope diagnosis coincidence rate was 90.14%(128/142),and cytology

  2. Diagnostic value of cervical cytological examination, HPV-DNA test combined with colposcopy examination in cervical lesions diagnosis%宫颈细胞学检查、HPV-DNA检测配合阴道镜检查对子宫颈病变的诊断价值

    Institute of Scientific and Technical Information of China (English)

    李玉欢

    2013-01-01

    目的 分析与探讨宫颈细胞学检查、HPV-DNA(人乳头瘤病毒基因检测)检测配合阴道镜检查对于子宫颈病变的诊断价值.方法 选取本院2010年8月至2012年8月期间收治的接受子宫颈病变筛查的妇女共1020例,对其实施宫颈细胞学检查和HPV-DNA配合阴道镜检查,检查结果与组织学金标准进行比对,以对比两种方法的准确性与诊断价值.结果 采取HPV-DNA配合阴道镜检查为异常结果的检出率较宫颈细胞学检查更高,差异具有统计学意义(P<0.05),前者检出异常共64例,后者检出异常共34例.结论 宫颈细胞学检查、HPV-DNA检测以及阴道镜检查对于子宫颈病变均有一定的诊断价值,而采用HPV-DNA检测配合阴道镜检查能够帮助医生较早地发现患者的宫颈癌前病变,为患者的治疗方案的选择提供科学依据.%Objective To analyze and discuss the diagnostic value of cervical cytological examination,HPV-DNA test combined with colposcopy examination in diagnosis of cervical lesions.Methods From August 2010 to August 2012 in our hospital a total of 1020 cases of women who underwent cervical diseases screening,were implemented with cervical cytological examination and HPV-DNA combined with colposcopy examination,the correlated pathological results were compared with the gold standard in accuracy and diagnostic value of two methods.Results The HPV-DNA abnormal results detection rate was higher than that of cervical cytological examination combined with colposcopy examination,with statistically significant difference,former found 64 abnormal cases,the latter examination found 34 cases of abnormal.Conclusion Cervical cytological examination,HPV-DNA testing combined with colposcopy examination for cervical lesions has certain diagnostic value,and the combined use of HPV-DNA testing and colposcopy examination could help doctors find early precancerous lesion of cervical cancer,and provide scientific basis for the

  3. 深圳社区女性宫颈病变防治认知情况调查分析%Questionnaires survey on the cognition of prevention and treatment for cervical lesions among females from Shenzhen communities

    Institute of Scientific and Technical Information of China (English)

    周艳秋; 杨将; 吴瑞芳

    2016-01-01

    higher in the females who were in the age of 31-40 years old, with more than 30 thousand income, with college education or above degree, civil servants or office clerks (P<0.05).Conclusions The females from communities have some knowledge about the relationship between cervical cancer and HPV, but lack comprehensive understanding of prevention and treatment of cervical cancer. Especially we need to enhance the diverse health knowledge spread in females who are young, with low-education background, with low income and labors or farmers, improving the awareness rate of prevention and treatment for cervical lesions.

  4. The research progress of vagina lactobacillus is associated with cervical lesions%阴道乳杆菌与宫颈病变相关的研究进展

    Institute of Scientific and Technical Information of China (English)

    辛秀团

    2015-01-01

    The cervical lesion is one of the major diseases threatening the health of women, in the context of its incidence, second only to breast cancer in female malignant tumor in the whole world, in some countries even than the breast cancer incidence of a disease. Mainly concentrated in cervical lesions in patients with age between 30 to 45 years old, and as the patient age incidence is obviously rising trend. At home and abroad at present, many scholars believe that the cervical lesions have direct links to the fall in the number of lactobacillus in the vagina, aimed at this situation, this study from the antitumor mechanism and original microbial antagonism disease-resistant two aspects of lactobacillus in the vagina and cervix lesion of correlation analysis, the peers to play the role of reference and reference.%宫颈病变是威胁妇女健康的主要疾病之一,就其发病率来看,在全球女性恶性肿瘤中仅次于乳腺癌,在部分国家中发病率甚至超过了乳腺癌。宫颈病变患者年龄主要集中在30-45岁之间,而且随着患者年龄的增长发病率呈明显上升趋势。目前,国内外诸多学者认为宫颈病变与阴道乳杆菌数量下降有着直接的联系,针对这一现状,本研究从抗肿瘤机制以及拮抗病原微生物两个方面对阴道乳杆菌与宫颈病变的相关性进行分析,希望能够对广大同行起到借鉴与参考的作用。

  5. 宫颈病变患者人乳头瘤病毒16亚型E2基因多态性检测%Detection of E2 gene polymorphism of human papillomavirus type 16 in patients with cervical lesions and cervical cancer

    Institute of Scientific and Technical Information of China (English)

    邱峰; 袁丽娜; 黄宪章

    2012-01-01

    Objective To investigate the incidence of human papillomavirus type 16 (HPV16) infection in patients with cervical diseases and analyze E2 gene mutations and its correlation with cervical lesions. Methods Specific primers were designed according to the full-length E2 gene sequence of HPV16 from the GenBank. PCR was used to analyze the incidence of HFV16 infection in the exfoliated cell samples from 379 patients with different cervical diseases positive for high-risk HPV. The polymorphisms of E2 gene of HPV16 were analyzed by high-resolution melting. Results Of the 379 exfoliated cell samples from the cervix, 78 were positive for HPV16 infection. The positivity rates of HPV16 infection was 44,8% in patients with cervical cancer, 31.5% in patients with CIN Ⅱ-Ⅲ, 241% in patients with CIM I and 9.6% in patients with cervical inflammation. The frequencies of E2 gene 68C and 133G variations were significantly higher in patients with CIN Ⅱ-Ⅲ and those with cervical cancer than in those with CIN I and those with cervical inflammation (P<0.05). Conclusion HPV16 is an important cause of cervical cancer, and HPV16 infection rate increases with the pathological progression of the cervical lesions. The naturally occurring sequence variations of HPV16 E2 gene may cause alterations uf the carcinogenic potential of the virus,%目的 分析宫颈病变中人乳头瘤病毒(HPV) 16亚型E2基因的多态与宫颈病变的关系,了解E2基因变异情况及其与宫颈病变的相关性.方法 应用PCR和高分辨率熔解曲线方法针对379例HPV高危亚型阳性宫颈脱落细胞样本进行HPV16感染情况及HPV16亚型E2基因68位点和133位点多态分布情况进行检测.结果 379例HPV高危亚型阳性宫颈标本共检出78例HPV 16亚型附性,其在宫颈癌、CINⅡ~Ⅲ、CIN Ⅰ、炎症患者中的检岀率分别为44.8%、31.5%、24.1%和9.6%;HPV16 E2基因68C和133G的频率在中重度宫颈内瘤样病变和宫颈癌中明显高于轻度

  6. Study on the relationship between high risk human papilloma virus infection and cervical cancer and precancerous lesions%高危人乳头瘤病毒感染与宫颈癌及癌前病变的关系探讨

    Institute of Scientific and Technical Information of China (English)

    余锦芬

    2012-01-01

    目的 探讨人乳头瘤病毒(HPV)感染与宫颈癌、癌前病变的关系及不同亚型HPV病毒重叠感染对宫颈癌、癌前病变的影响.方法 以148例宫颈癌及癌前病变患者为观察组并以96例宫颈炎患者为对照组,观察两组研究对象高危HPV病毒感染率、多重感染对宫颈癌、癌前病变的影响及不同病理类型宫颈癌高危HPV感染率.结果 HPV病毒感染率以鳞癌、腺癌居多;高危型HPV病毒感染率、双重感染率、多重感染率在宫颈癌中较高,CINⅢ期、CINⅡ期、CIN Ⅰ期依次降低,均高于宫颈炎中感染率.结论 高危型HPV病毒感染与宫颈癌的发生、发展密切相关,阻断高危型HPV病毒的持续感染可阻断癌前病变的进程,是防治宫颈癌的关键所在.%OBJECTIVE To explore the relationship between human papilloma virus (HPV) infection and cervical carcinoma, precancerous lesion and study the effects of different subtypes of HPV virus superinfection on cervical cancer, precancerous lesion. METHODS 148 cases of cervical cancer and precancerous lesions of patients in observation group, and 96 cases of cer-vicitis patients as control group, observed high risk HPV infection rate, the effect of multiple infection on cervical cancer and precancerous lesions, and high risk HPV infection rate of different pathological types of cervical cancer in subjects of two groups. RESULTS HPV virus infection rates were more in squamous cell carcinoma, adenocarcinoma; high-risk HPV infection rates, double infection rates, multiple infection rates was higher in cervical cancer, and in stage Ⅲ , CIN, CIN Ⅱ , CIN Ⅰ lowered in turn, they were higher than in the infection rate of cervicitis. CONCLUSION The high risk HPV virus infection is closely associated with occurrence and development of cervical cancer. Blocking high risk HPV virus persistent infection can block precancerous lesions of the process, it's the key to prevent and treat cervical cancer.

  7. Analysis of the Relationship Between HPV Infection and Cervical Lesions and the Related Risk Factors%HPV感染与宫颈病变的关系及相关危险因素分析

    Institute of Scientific and Technical Information of China (English)

    张桢华; 孙翠梅; 刘春君

    2016-01-01

    目的:探讨HPV感染与宫颈病变关系,分析HPV感染的相关危险因素。方法选取2013年5月~2015年5月就诊于我院妇科并行阴道镜检查、TCT筛查、宫颈活组织检查的216例患者为研究对象。依据宫颈活检病理学诊断,分为研究组(患有宫颈病变,141例)和对照组(宫颈正常,75例),并对同时期就诊于我院妇科的196例签署知情同意书的患者进行临床资料查阅及问卷调查,回顾分析所有患者的临床、生化及问卷调查资料。结果正常宫颈、CINⅠ级、CINⅡ~CINⅢ级、宫颈癌患者HPV感染率分别为16.0%、67.1%、83.7%、95.5%,经统计分析显示正常宫颈组与宫颈病变组各病理级别HPV感染率差异有统计学意义(P<0.05),宫颈病变不同病理级别间HPV感染率比较差异也有统计学意义(P<0.05);对患者一般资料与HPV感染的关系进行分析,结果显示:年龄、受教育程度、首次性生活年龄、避孕方式差异均具有统计学意义(P<0.05)。结论HPV感染与宫颈病变病理级别呈正相关;年龄较大(>50岁)、受教育程度低(高中以下)、首次性生活年龄小(≤20岁)是HPV感染的危险因素,非避孕套避孕可增加HPV感染可能性;抗HPV生物蛋白敷料可用于治疗HPV感染,对于宫颈高危HPV感染及其引起的低级别宫颈病变均有明显治疗效果。%Objective To explore the relationship between HPV infection and cervical lesions, and analyze the related risk factors for HPV infection. Methods216 cases of routine vaginal examination, TCT screening, cervical biopsy were selected from May 2013 to May 2015 in our hospital, TCT screening. Based on cervical biopsy pathology, is divided into research group (suffering from cervical lesions, 141 cases) with cervical lesions, and the control group (normal cervix, 75 cases), and at the same time to see a doctor in the hospital of department of

  8. Performance of visual inspection with acetic acid and human papillomavirus testing for detection of high-grade cervical lesions in HIV positive and HIV negative Tanzanian women

    DEFF Research Database (Denmark)

    Dartell, Myassa Arkam; Rasch, Vibeke; Iftner, Thomas;

    2014-01-01

    The aim of this cross sectional study was to assess type distribution of human papillomavirus (HPV) among HIV positive and HIV negative women who underwent cervical cancer screening, and to examine the ability of visual inspection with acetic acid (VIA), the standard detection method in Tanzania...

  9. A shift to a peripheral Th2-type cytokine pattern during the carcinogenesis of cervical cancer becomes manifest in CIN III lesions

    NARCIS (Netherlands)

    A.G. Bais (Aagje); I. Beckmann (Ilse); J. Lindemans (Jan); P.C. Ewing (Patricia); C.J.L.M. Meijer (Chris); T.J.M. Helmerhorst (Theo); P.J.L.M. Snijders (Pieter)

    2005-01-01

    textabstractBACKGROUND: A shifted balance between T helper 1 (Th1)-type and Th2-type cytokines has been hypothesised in cervical dysplasia. AIMS: To evaluate possible deregulation of the cytokine network by estimating the expression of peripheral cytokines in different stages of ce

  10. 高危型人乳头瘤病毒阳性与宫颈病变关系的研究%The Research of High-risk Human Papillomavirus and Cervical Lesions Positive Relationship

    Institute of Scientific and Technical Information of China (English)

    王西兵

    2014-01-01

    Objective In recent years female cervical lesions continued to rise on, a serious threat to the women's physical and mental health, clinical began inducing factors of cervical lesions and treatment. Analysis of the relationship between positive for high-risk human papillomavirus and cervical lesions, and provide reliable suggestions for clinical treatment, the accumulation of clinical experience in the treatment of more. Methods The study selection for the 2010 June-2013 year in June, our hospital included female patient data, through the clinical comprehensive diagnosis of cervical lesions, the implementation of the necessary checks on high-risk human papillomavirus positive, to determine the specific situation of the cervical lesions. In addition to pathological examination, but also the implementation of a comprehensive examination of cervical cancer patients, mainly state the clinical disease, to detect the high-risk human papillomavirus types as the basis of practical treatment options. Results ①the case for relates to the number of 6096 cases, high-risk human papillomavirus positive were 1351 cases, the positive rate of the statistical results of high-risk human papillomavirus 22.3%;look from the age, the positive rate from high to low according to the arrangement of 25-29 years, 55-59 years, 40-45 years old, the positive rate were 29.9%, 26%, 15.3%;the positive rate of comprehensive comparison of several age groups, the results have significant difference(P<0.01).②according to the statistical results, the high-risk human papillomavirus positive cases, choose the colposcopic biopsy of 1187 cases, the final results showed 54 cases of normal cervical organizers, 833 cases of chronic cervicitis, 297 cases of cervical intraepithelial neoplasia. Take the same colposcopy examination, according to the diagnostic criteria of Chinese Medical Association Rules, the passing rate of 94.7%;in accordance with the pathology compared with other detection results theory

  11. Molecular epidemiology of human papillomavirus infections among cervical lesions women in Quzhou area%衢州地区宫颈病变女性人乳头状瘤病毒感染分子流行病学研究

    Institute of Scientific and Technical Information of China (English)

    余晓; 夏利花; 徐小敏; 周燕

    2013-01-01

    OBJECTIVE To study the molecular epidemiological characteristics of 21 genotypes of human papillomavirus (HPV) among 1280 women with cervical lesions in Quzhou area. METHODS The 21 HPV types were determined among 1280 women who suffered from cervical lesions in Quzhou area. The molecular epidemiological characteristics of 21 HPV genotypes were compared. RESULTS Of 1280 cases with cervical lesions, HPV infections occurred in 708 cases with the infection rate of 55. 31% , the patients with high-risk HPV infections accounted for 38. 83% , and the patients with low-risk HPV infections accounted for 16. 48%. HPV16, 58 and 52 were the most common high-risk genotypes, and HPV6 and 11 were the most common low-risk genotypes. The total HPV infection rate and the high-risk infection rate of the patients aged between 31 and 40 years were the highest, which were 67. 39% and 50. 58% , followed by the patients aged between 21 and 30 years, which were 61. 75% and 45. 78% respectively, the differences in the proportion of HPV infections and high-risk HPV infections between the various age groups of patients were statistically significant. CONCLUSION This study has offered the molecular epidemiological characteristics of 21 genotypes of HPV among 1280 cervical lesions women, providing reliable and scientific evidence for the clinical prevention of cervical cancer, it is of great value in development and application of HPV vaccine.%目的 分析衢州地区1280例宫颈病变女性中人乳头状瘤病毒(HPV) 21种基因型的分子流行病学特点.方法 对衢州地区1280例宫颈病变的女性进行21种HPV基因型分型检测,比较不同基因型的流行病学特点.结果 1280例宫颈病变病例中HPV感染708例,感染率为55.31%,其中高危型HPV感染率为38.83%,低危型HPV感染率为16.48%,高危型最常见类型为HPV16、58、52,低危型最常见类型为HPV6、11;31~40岁组HPV总感染率及高危型感染率最高,分别占67.39%和50.58

  12. Asociación entre la presencia de anticuerpos anti-Ras y anti-VPH16 E4/E7 y lesiones intraepiteliales del cérvix Association between anti-Ras and anti-HPV16 E4/E7 antibodies with cervical intraepithelial lesions

    Directory of Open Access Journals (Sweden)

    Sara Vázquez-Corzo

    2003-10-01

    Full Text Available OBJETIVO: Determinar si anticuerpos séricos contra E4, E7 y Ras pueden ser utilizados como marcadores de lesiones tempranas del cérvix uterino asociadas al virus del papiloma humano. MATERIAL Y MÉTODOS: Entre marzo de 1999 y abril de 2000 se realizó un estudio sero-epidemiológico de casos y controles en la clínica de displasias del Hospital General Doctor Gea González, en la Ciudad de México, en 116 muestras de suero para evaluar la presencia de anticuerpos anti-E4, E7 y Ras utilizando un ELISA de captura. Se estimaron razones de momios e intervalos de confianza de 95% RESULTADOS: Anticuerpos anti-E7 se asociaron a mujeres con lesiones NIC III, mientras que anticuerpos anti-E4 y anti-Ras fueron más frecuentes en lesiones NIC I-II. Al evaluar el perfil de anticuerpos que presentaron las mujeres, encontramos que a anticuerpos contra dos proteínas predicen la existencia de una lesión NIC I-II, y b la presencia de tres anticuerpos predicen una lesión NIC III. CONCLUSIONES: La detección de anticuerpos séricos contra E4, E7 y Ras en combinación con otras técnicas de diagnóstico, podrían ser de utilidad para detectar oportunamente a mujeres con lesiones tempranas asociadas al Virus del Papiloma Humano y en riesgo de desarrollar cáncer.OBJECTIVE: To evaluate whether serum antibodies anti-E4, E7 and Ras could be used as markers for early cervical lesions associated with HPV (human papillomavirus. MATERIAL AND METHODS: A seroepidemiological case-control study was conducted between March 1999 and April 2000 at the dysplasia clinic of Hospital General Doctor Gea Gonzalez, in Mexico City, to evaluate the presence of antibodies anti-E4, E7, and Ras through a sandwich ELISA. Analysis was done using odds ratios and 95% confidence intervals. RESULTS: Anti-E7 antibodies were associated to women with CIN III lesions, while anti-E4 and Ras antibodies were strongly associated with CIN I-II lesions. The antibody profile of women with different

  13. 人乳头瘤病毒基因型与宫颈病变的相关性分析%Correlation analysis of human papilloma virus genotypes and cervical lesions

    Institute of Scientific and Technical Information of China (English)

    黄秀珍; 姚心韵; 陈文学; 吕赛平

    2015-01-01

    目的:探讨不同基因型人乳头瘤病毒(human papilloma virus, HPV)感染与宫颈病变的相关性。方法采用导流杂交基因芯片技术(Hybrimax)对924例妇女进行21种HPV基因型检测,同期进行宫颈活检病理检查。按病理学诊断结果分成6组:宫颈鳞癌组287例;宫颈上皮内瘤变(CIN)Ⅲ组78例;CINⅡ组61例;CINⅠ组153例;宫颈湿疣组40例;正常或炎症组305例。结果⑴503例检测到HPV感染,阳性率为54.4%。所有标本共检测到19种HPV基因型,未检测到HPV43、44型。不同病变组HPV阳性率差异有统计学意义(P<0.05),宫颈鳞癌组与宫颈湿疣组HPV阳性率显著高于CINⅢ组(P<0.05),CINⅢ组HPV阳性率显著高于CINⅡ组与CINⅠ组(P<0.05),CINⅡ组与CINⅠ组HPV阳性率显著高于正常或炎症组(P<0.05)。⑵不同病变组HPV多重感染率差异有统计学意义(P<0.05),宫颈鳞癌组、CINⅢ组、CINⅡ组、CINⅠ组HPV多重感染率显著高于湿疣组与正常或炎症组(P<0.05)。结论不同类型宫颈病变与HPV感染率、HPV感染型别有着一定的关系, HPV多重感染可能是宫颈鳞癌以及宫颈上皮内瘤变的危险因素。%Objective To investigate the correlation between the infection of different genotypes of human papilloma virus (HPV) and cervical lesions. Methods Twenty-one kinds of HPV genotypes were detected in 924 cases of women by flow-through hybridization and gene chip technology, and the cervical biopsy was performed at the same time. According to the results of patho-logical diagnosis, these women were divided into 6 groups, including cervical squamous cell carcinoma group (287 cases), cervical intraepithelial neoplasia (CIN)Ⅲ group(78 cases), CINⅡ group(61 cases), CINⅠ group (153 cases), cervical condyloma group (40 cases), normal or inflammation group (305 cases). Results A total of 503 cases were HPV-positive, and the positive rate was 54.4%. 19 kinds of HPV genotypes

  14. 液基薄层细胞学检测在宫颈上皮内瘤变和宫颈癌诊断中的应用价值分析%The application of TCT in the diagnosis of cervical epithelial lesions and cervical cancer

    Institute of Scientific and Technical Information of China (English)

    韩一栩; 曾新

    2015-01-01

    Objectives:To investigate the application value of TCT in the diagnosis of cervical epithelial le-sions and cervical cancer.Methods:681 patients that received cervical lesions screening in our department from June 201 2 to December 201 3 were selected and were tested by TCT and colposcopy cervical biopsy.The relationship between age and incidence rate was explored,and results of TCT cytological and histological diagnosis were com-pared.Results:The positive rate of TCT was higher in patients of 36 ~45 years old.There were ASC -H (60 ca-ses),ASC -US (222 cases),LSIL(89 cases),HSIC (1 4 cases),SCC (5 cases)and AGC (2 cases)diagnosed by TCT cytology,and 1 78 cases of CINI,1 1 4 cases of CIN II, 33 cases of CIN III and 1 3 cases of SCC diagnosed by TCT.Choosing histological diagnosis as the gold standard,the coincidence rate of ASC -H,ASC -US,LSIL, HSIC and SCC were 91 .67% (55 /60),34.23% (76 /222),87.64% (78 /89),92.86% (1 3 /1 4)and 1 00%(2 /2)respectively.Conclusion:With a high positive rate,TCT is an important screening means for cervical intra-epithelial lesions,which is conducive to early diagnosis and treatment of cervical intraepithelial neoplasia and cervi-cal carcinoma.%目的:探讨液基薄层细胞学技术(TCT)在诊断宫颈上皮内瘤变及宫颈癌中的应用价值。方法:选取2012年6月至2013年12月期间在我院接受宫颈病变筛查的681例患者作为研究对象,行 TCT检查及阴道镜下宫颈活检,探讨年龄与发病率的关系,同时对比研究 TCT 细胞学与组织学诊断结果。结果:TCT 阳性率多见于36~45岁年龄段;TCT 细胞学诊断 ASC -H 60例,ASC -US 222例,LSIL 89例,HSIC 14例,SCC 5例,AGC 2例;组织学诊断 CINI 178例,CINⅡ114例,CINⅢ33例,SCC 13例,以组织学诊断为金标准,ASC -H 符合率为91.67%(55/60),ASC -US 为34.23%(76/222),LSIL 为87.64%(78/89),HSIC 为92.86%(13/14),SCC 为100%(2/2)。

  15. p16INK4a在宫颈病变中的研究进展%Progress of P16INK4a in Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    刘莹; 周瑾

    2011-01-01

    宫颈癌严重威胁女性健康和安全,是导致女性死亡的主要恶性肿瘤之一.近年来我国宫颈癌的发病率正以每年2%~3%的速度增长,因此早期诊断对于预防和治疗宫颈癌具有决定性的意义.p16INK4a是近年来发现的肿瘤抑制基因,是一种细胞周期蛋白D依赖性激酶的抑制剂,研究表明p16INK4a的表达与宫颈癌的发生发展密切相关.目前宫颈癌筛查中的细胞学检查及hr-HPV检测方法 都具有一定的局限性,寻求新的筛查方法 已成为研究热点.p16INK4a的检测技术易于普及、操作简便,不仅能提高宫颈癌的早期诊断率、预测宫颈癌的发生发展,且较HC-Ⅱ检测更能区分是否存在病变,从而降低传统宫颈癌筛查的假阳性率及假阴性率,提高筛查的灵敏度和特异度,为宫颈癌的筛查开辟了新途径,值得进一步深入研究.%Cervical cancer was a serious threat to women's health and safety , which was the leading cause of cancer death for women. In recent years,the incidence of cervical cancer increased 2% -3% per year in China,so an early diagnosis to prevent cervical cancer and the treatment was of decisive importance. As a tumor suppressor gene,pl6INK4a was a recently discovered inhibitor of cyclin D-dependent kinase; studies had shown that the expression of pl6INK4a was closely linked to the development of cervical cancer. Current screening of cervical cytology and detection methods hr-HPV had certain limitations,to seek new screening method had become a research hotspot.pl6INK4a detection technology was easy to spread,easy to use,which can not only improve the early diagnosis of cervical cancer to predict the impact of the development of cervical cancer, and was better than the HC- II test to distinguish the existence of the disease,thus reducing false positives traditional cancer screening cervical rate and false negative rates,and improving the sensitivity and specificity of screening for cervical

  16. Clinical value of liquid-based thinPrep cytology test and cervical biopsy in the diagnosis of cervical Squamous epithelial lesions%液基薄层细胞学检测联合宫颈活检对诊断宫颈鳞状上皮病变的临床价值

    Institute of Scientific and Technical Information of China (English)

    赵瑞皎; 石曼丽; 张家兴; 孔令非

    2012-01-01

    目的:探讨液基薄层细胞学检测(liquid-based thinPrep cytology test,TCT)联合宫颈活检对诊断宫颈鳞状上皮病变的临床价值.方法:应用TCT对30350例受检者进行宫颁细胞学检查,细胞学检查结果为鳞状上皮异常者,进行阴道镜活检及病理检查.结果:TCT检出鳞状上皮异常者1 824例(6.01%),其中无明确意义的不典型鳞状细胞(atypical squamous cells of undetermined significance,ASC-US)1423例,不除外高度鳞状上皮病变的不典型鳞状细胞(atypical squamous cells cannot exclude high grade intraepithelial lesion,ASC-H)214例,低度鳞状上皮内病变(low grade squamous intraepithelial lesion,LSIL) 92例,高度鳞状上皮内病变(high grade squamous intraepithelial lesion,HSIL) 80例,鳞状细胞癌(squamous cell carcinoma,SCC) 15例.与活检病理检测结果相比,1423例ASC-US中,宫颁上皮内瘤样病变-Ⅰ级(grade Ⅰ cervical intraepithelial neoplasia,CIN-Ⅰ)202例、CIN- Ⅱ和CIN- Ⅲ 22例、SCC 1例;214例ASC-H中,CIN-Ⅰ 12例、CIN-Ⅱ和CIN-Ⅲ 101例、SCC5例:LSIL、HSIL及SCC组中与组织病理检测结果的符合率分别为63.04%( 58/92)、81.25% (65/80)及100%( 15/15),SCC组和HSIL组的组织学符合率高于LSIL组(P<0.01).结论:TCT与阴道镜活检病理检测结果有较高的符合率,二者联合能提高宫颈癌前病变及癌变的检出率.%Objective : To investigate the clinical value of liquid-based thinPrep cytology test (TCT) and cervical biopsy in the diagnosis of cervical squamous epithelial lesions. Methods: TCT was performed in 30 350 subjects, and the colposcopy-directed biopsy and histopathologicai examination were performed when the TCT revealed abnormal cervical squamous epithelial lesions. Results: TCT results revealed that 1 824 (6.01%) cases had abnormal cervical squamous epithelial lesions, including 1 423 cases of atypical squamous cells of undetermined significance (ASC-US), 214 cases of atypical squamous cells cannot

  17. 液基薄层细胞检测系统与阴道镜下活检在诊断子宫颈病变中的价值%Value of Liquid-Based Thin-Layer Cell Detection System and Cervical Biopsy Vaginoscopy in Diagnosis of Cervical Lesion

    Institute of Scientific and Technical Information of China (English)

    王美玲; 王薇; 周新娥; 吴逢霞; 蒋亚男

    2009-01-01

    Objective To study the diagnostic value of liquid-based thin-layer cell detction system(LCT)and cervical biopsy vagionscopy for cervical intraepithelial neoplasia(CIN).Method The clinical information of 7118 patients that received LCT and 778 cases of positive cytological result that received cervical biopsy were retrospectively analyzed.167 patients with negative result in cytological test received cervical biopsies vaginoscopy because of their high-grade clinical suspicion.Results In 7118 patients,429 cases was diagnosed as CIN by cervical biopsy,the diagnosis rate was 6.03%(429/7118).In 167 patients,78 cases of 778 cases that were suggested as untypical squamous cell lesions by LCT were diagnosed as CIN by cervical biopsy.The total diagnosis rate is 7.12%[(429+78)/7118].There was a significant difference between them(P<0.01).Conclusions LCT could detect cervical lesion in its early stage.The patients whose cytological test results are positive or clinical high-grade suspicious should receive cervical biopsy vaginoscopy,which may benefit to the increase of detection rate of CIN.%目的 探讨液基薄层细胞检测系统(LCT)联合阴道镜及阴道镜下宫颈活检对宫颈上皮内瘤变(CIN)的诊断价值.方法 回顾性分析7118例LCT筛查结果及其中778例细胞学阳性行阴道镜下活检的病理结果,167例虽细胞学阴性,但临床高度可疑,行阴道镜检出并取活检.结果 7118例患者中,778例LCT检查提示不典型鳞状细胞以上病变者,再行阴道镜下活检发现CIN以上病变429例,检出率为6.03%(429/7118).167例LCT检查阴性但临床高度可疑病例,行镜下活检CIN以上病变78例,总检出率为7.12%[(429+78)/7118].两者比较差异显著(P<0.01).结论 采用LCT筛查可早期发现宫颈病变,细胞学阳性或临床可疑者应配合阴道镜检查及镜下活检可提高CIN的检出率,但如果同时进行阴道镜检查可提高CIN的检出率.

  18. Study of the interaction of the risk factors in cervical cancer risk degree and the relationship between HPV genotype and cervical cancer and precancerous lesions%危险因素与宫颈癌发生危险度的交互作用及HPV基因型与宫颈癌及其癌前病变关系的研究

    Institute of Scientific and Technical Information of China (English)

    杨丽霞; 董剑梅

    2015-01-01

    目的:研究危险因素与宫颈癌发生危险度的交互作用及HPV基因型与宫颈癌及其癌前病变关系.方法:宫颈癌患者以及对照组共645例,对其相关资料给予回顾性分析.结果:性传播疾病史、性伴侣数、孕次是引发宫颈癌的危险因素,可是交互作用差异不具有统计学意义(P>0.05);感染以后 CIN Ⅰ危险性较高的 HPV 基因型为HPV-16,HPV-18,HPV-31,HPV-33,HPV-45,HPV-53,HPV-58,HPV-59.结论:宫颈癌行为危险因素与HPV 感染有联合作用.性传播疾病史以及性伴侣数的联合作用增加宫颈癌的发生风险.另外,HPV 感染当中, HPV-52是最为流行的亚型,其次为HPV-16,HPV-18,HPV-33,HPV-53.%Objective:To study the interaction of the risk factors in cervical cancer risk degree and the relationship between HPV genotype and cervical cancer and precancerous lesions.Methods:The patients with cervical cancer and the control group were 645 cases.The related data were given retrospective analysis.Results:The sexually transmitted diseases history,sexual partners number and gravidity were the risk factors for cervical cancer,but the difference of interaction was not statistically significant(P>0.05). The HPV genotypes with higher CIN Ⅰrisk degree after infection were HPV-16,HPV-18,HPV-31,HPV-33,HPV-45,HPV-53, HPV-58,HPV-59.Conclusion:The behavior risk factors of cervical cancer and HPV infection have a combined effect.The combined effect of sexually transmitted diseases history and sexual partners number can increase the occurrence risk of cervical cancer.In addition,in the HPV infection,HPV-52 is the most popular subtype,followed by HPV-16,HPV-18,HPV-33,HPV-53.

  19. Towards a new understanding of cervical glandular epithelial lesions:pathological perspectives%宫颈腺上皮病变病理学相关问题及其研究进展

    Institute of Scientific and Technical Information of China (English)

    刘从容

    2016-01-01

    In May 2014, World Health Organization (WHO ) released WHO (2014 ) Classification of Tumor of the Female Reproductive Organs.According to the classification of tumors precursor lesion of cervical adenocarcinoma of female reproductive system in WHO (2014 ) Classification of Tumor of the Female Reproductive Organs,and the latest fundamental research progress of human papillomavirus (HPV)new developments,historical perspectives and controversial issues about the cervical glandular epithelial precursors will be presented.%结合2014年5月,世界卫生组织(WHO)发布的《WHO(2014)女性生殖系统肿瘤分类》中,宫颈腺癌前驱病变分类与人乳头状瘤病毒(HPV)感染最新基础研究进展,对宫颈腺上皮病变的相关病理学问题及其研究进展进行简要介绍。

  20. Infecção por Chlamydia em pacientes com e sem lesões intra-epiteliais cervicais Chlamydia infection in patients with and without cervical intra-epithelial lesions

    Directory of Open Access Journals (Sweden)

    Micheline de Lucena Oliveira

    2008-12-01

    and without intraepithelial cervical lesions attended at specialized ambulatory in Recife (2007 and its association with biologic and demographic variables, habits, reproductive, clinical and gynecologic characteristics. METHODS: A cross-sectional study was conducted including 70 women (35 with cytological cervical lesions and 35 with normal smears. Colposcopy and biopsy when necessary were performed and Chlamydia infection was determined by direct immunofluorescence. Analysis variables were age, race, precedence, schooling, marital status, menarche, age at first sexual intercourse, parity, number of sexual partners, vaginal discharge, previous Pap smear, STD episodes, cervical cauterization, contraceptive methods, familial antecedents of cervical cancer, alcohol intake, use of illicit and immunosuppressive drugs, Papanicolaou result and cervical Chlamydia l infection. To determine the strength of association prevalence ratio (PR and its 95% confidence interval were calculated. Multivariate analysis was performed for controlling potentially confounding variables. RESULTS: the frequency of Chlamydia l infection was significantly greater in patients with intraepithelial cervical lesions (80.0% vs. 14.3%; PR = 5.60; 95% CI = 2,44 - 12,82. When the factors associated with Chlamydia l infection were analyzed, the only variable that remained significantly associated after multivariate analysis was previous episode of STD (OR=63,47; 95% CI = 13,93 - 289,09. CONCLUSION: Chlamydia trachomatis infection is associated with intraepithelial cervical lesions and history of STD should be considered for treatment and follow-up of these patients.

  1. 女性不同人群宫颈病变发病状况调查及危险因素分析%Incidence and Risk Factors of Cervical Lesions among Different Famale People

    Institute of Scientific and Technical Information of China (English)

    徐又先; 袁林; 陈广莉

    2012-01-01

    Objective To investigate the incidence of cervical lesions in urban,non-resident and rural population of Liuzhou area and to analyze its relationship with living and sanitation habits. Methods Living and sanitation habit were examined using questionnaire and investigation of the high risk factor of the general condition, the result of gynecology physical examination,analysis of the risk factors and the pathological types of cervical lesions. Results Education level,using tubbing,number of sex partner,smoking, drinking,vaginal douching, washing vulva during menstruation, frequency of vitamin food consumption, were statistically different among three populations. The incidence of CIN was 6. 11%, 11.60% and 10. 37% .respectively. Nonresident and rural females were more popular in urban women, and HPV infection was highest in rural female (29. 0%). The difference of the incidences of HPV and CIN were found among three populations (P<0. 05). After multiple factor non-conditional Logistic regression analysis, early age of first intercourse,multiple sexual partner were the risk factors of cervical lesions. Smoking and drinking showed no correlation with cervical lesions. Conclusion Different living and sanitation habit of urban,rural and non-resident populations may contribute to the difference in incidence of CIN. Individual behavior intervene, especially douching forbidden should be done to reduce the prevalence of CIN.%目的 了解城市人群、流动人群、农村人群宫颈病变发病状况及不同生活、卫生行为对宫颈病变发病的影响.方法 对三组人群行宫颈病变调查和妇检,并行高危因素分析.结果 三组教育程度、盆浴、性伴侣数、吸烟、饮酒、阴道冲洗、经期清洗外阴和食维生素类食物情况差异有统计学意义;宫颈病变检出率分别为6.11%、11.60%、10.37%,流动和农村组高于城市组;HPV感染农村人群(29.0%)和流动人群(21.7%)明显高于城市人群(11.0

  2. TCT联合DNA定量细胞学检查对宫颈病变临床诊断价值研究%The study of TCT and DNA quantitive cytology in the diagnosis of cervical lesions.

    Institute of Scientific and Technical Information of China (English)

    刘桂依; 黄艳美

    2011-01-01

    目的 评价宫颈细胞学配合DNA定量细胞学检查对子宫颈病变的诊断价值.方法 2006年2月至2007年12月间在我们中心对2 800例患者行液基薄层细胞学检查(TCT)及DNA定量细胞学检查.TCT或DNA定量细胞学检查异常者,3个月后复查.TCT未明确诊断意义的不典型鳞状上皮细胞(ASCUS)异常者行阴道镜下多点活组织病理检查.结果 TCT异常者302例,占10.79%(302/2 800).ASCUS 194例,占6.93%;低度鳞状上皮内病变(LSIL)34例,占1.21%;高度鳞状上皮(HSIL)内病变18例,占0.64%(18/2 800);鳞癌(SCC)3例,占0.10%.经阴道镜下多点活组织病理检查,诊断符合率较高.结论 采用TCT配合细胞DNA定量分析,因取材方便无创伤,是进行阴道镜下活检前筛查宫颈癌和癌前病变的好方法,可以帮助早发现宫颈癌和宫颈病变.%Objective To evaluate the diagnostic value of cervical cytology combined with DNA quantitive cytology for cervical lesions.Methods From February 2006 to December 2007, 2,800 patients were examined with liquid - based ThinPrep cytologic test ( TCT ) and DNA quantitative cytology. The patients with abnormal TCT or DNA quantitative cytology results were re - examined after three months. The patients with abnormal TCT results and atypical squamous cells of uncertain significance ( ASCUS ) were further examined by colposcopy and multi - site biopsy examinations. Results Abnormal TCT results were found in 302 patients ( 10.79% ), ASCUS, low - grade squamous intraepithelial lesions ( LSIL ), high - grade squamous intraepithelial lesion ( HSIL ) and squamous cell carcinoma ( SCC ) were found in 194 ( 6.93% ), 34 ( 1.21% ),18 ( 0.64% ) and 3 patients ( 0. 10% ), respectively. Pathological examination confirmed inflammation in 198 cases, accounting for 65.56%( 198/302 ) of abnormal TCT. The difference in TCT and human papillomavirus ( HPV ) infection diagnosis was significant ( P < 0.01 ). Conclusion The combined examinations of

  3. 液基薄层细胞学检查配合阴道镜检查对子宫颈病变的诊断价值%The diagnostic value of thinprep cytology combined with colposcopy for cervical lesions

    Institute of Scientific and Technical Information of China (English)

    白杰

    2014-01-01

    Objective To investigate the diagnostic value of ThinPrep cytology(TCT) combined with colposcopy for cervical lesions.Methods 980 patients with suspected cervical lesions were selected and received TCT combined with colposcopy examination.Among the 980 patients,102 patients with positive smear were referred for colposcopy and were diagnosed by colposcopy biopsy.Results Of 102 patients with positive smear,the detection rates of the atypical squamous cells (ASC),low-grade squamous intraepithelial lesions (LSIL),cervical intraepithelial neoplasia (HSIL) and atypical glandular cells (AGC) were 6.1%,0.6%,2.1% and 1.5%,respectively.Compared with the colposcopy biopsy for cytology samples,the test results were as follows:LSIL was 72.4% (21/29),HSIL was 93.8% (15/16).Conclusion The method of TCT could increase the screening rate of positive diagnosis of cervical lesions and for the highly suspected patients,the combination of vaginal colposcopy and biopsy could further improve the accuracy of diagnosis,in order to provide scientific solutions for clinical treatment.%目的 探讨液基薄层细胞学检查(TCT)联合阴道镜检查对子宫颈病变的临床诊断应用价值.方法 选取疑似为宫颈病变的患者980例,采用TCT联合阴道镜检查,其中,送检样本阳性涂片患者102例,均行阴道镜检查,于阴道镜下取组织活检.结果 980例患者的102例阳性患者中,不典型鳞状上皮细胞(ASC)、低度鳞状上皮内病变(LSIL)、宫颈癌前病变(HSIL)及不典型腺细胞(AGC)的检出率分别为6.1%、0.6%、2.1%和1.5%;对细胞学阳性样本进行阴道镜组织活检,两种检测结果比较:LSIL为72.4% (21/29),HSIL为93.8% (15/16).结论 TCT筛查可增加宫颈病变阳性诊断率,对于高度疑似患者配合阴道镜检查及阴道组织活检可以进一步提高诊断的准确度,以便为临床治疗提供科学的方案.

  4. [Analysis of the reasons for abandoning the follow-up and treatment process in women with pre-cancerous cervical lesions in the province of Jujuy: implications for health management].

    Science.gov (United States)

    Paolino, Melisa; Arrossi, Silvina

    2012-12-01

    Adequate follow-up and treatment of women with high-grade squamous intraepithelial lesion (HSIL) and cancer is key in reducing cervical cancer mortality. This research study aims to analyze the magnitude of abandonment in the follow-up and treatment process, as well as the reasons for this abandonment, in women with HSIL who received care in public health services in the province of Jujuy in 2010. Secondary sources were analyzed and women were interviewed in their homes. The abandonment rate within the study population was 23.5%. Among these women, 40.5% mentioned organizational problems in the health care system as a reason for abandoning and 30.9% mentioned subjective reasons. Our results have been used in the reorganization of the follow-up and treatment process being carried out in the province. PMID:23681458

  5. A 7-year randomized prospective study of a one-step self-etching adhesive in non-carious cervical lesions. The effect of curing modes and restorative material

    DEFF Research Database (Denmark)

    van Dijken, Jan W V; Pallesen, Ulla

    2012-01-01

    Objective: The aim of this study was to evaluate the clinical retention of a one-step self-etching adhesive system (Xeno III) in Class V non-carious cervical lesions and the effect of restorative material and curing techniques on longevity of the restorations. Material and methods: A total of 139......-start or a pulse-delay curing mode. The restorations were evaluated at baseline, 6, 12, 18 and 24 months and then yearly during a 7 years follow-up with modified USPHS criteria. Dentin bonding efficiency was determined by the percentage of lost restorations. Results: During the 7 years, 135 restorations could...... be evaluated. No post-operative sensitivity was reported by the participants. Overall relative cumulative loss rate frequencies for the adhesive system at 6, 18 and 7 years, independent of curing technique and restorative material, were 0.8%, 6.9% and 23.0%, respectively. The self-etching adhesive fulfilled...

  6. Progress in abrasive and grinding technology

    CERN Document Server

    Xu, Xipeng

    2009-01-01

    The grinding and abrasive processing of materials are machining techniques which use bonded or loose abrasives to remove material from workpieces. Due to the well-known advantages of grinding and abrasive processes, advances in abrasive and grinding technology are always of great import in enhancing both productivity and component quality. In order to highlight the recent progress made in this field, the editor invited 21 world-wide contributions with the aim of gathering together all of the achievements of leading researchers into a single publication. The authors of the 21 invited papers, of

  7. Abrasion of flat rotating shapes

    OpenAIRE

    Roth, A.E.; Marques, C. M.; Durian, D. J.

    2010-01-01

    We report on the erosion of flat linoleum "pebbles" under steady rotation in a slurry of abrasive grit. To quantify shape as a function of time, we develop a general method in which the pebble is photographed from multiple angles with respect to the grid of pixels in a digital camera. This reduces digitization noise, and allows the local curvature of the contour to be computed with a controllable degree of uncertainty. Several shape descriptors are then employed to follow the evolution of dif...

  8. Cervical Pap smear study and detection of abnormal epithelial lesions and determination of its accuracy by cytohistological correlation in patients of tertiary care teaching hospital in central India

    Directory of Open Access Journals (Sweden)

    Garima Malpani

    2016-07-01

    Conclusions: Pap smear is a cost effective and sensitive screening method for detection of cancerous, pre-cancerous and non-cancerous lesions of cervix. [Int J Reprod Contracept Obstet Gynecol 2016; 5(7.000: 2312-2316

  9. Clinical Application of Colposcopy Cervical Biopsy and Liquid-based Cytology Test in the Screening of Cervical Lesions%阴道镜宫颈活检与 TCT 联合检测在宫颈病变中的应用

    Institute of Scientific and Technical Information of China (English)

    冷海娜; 孙名芳

    2013-01-01

    Objective To explore the clinical application value of colposcopy cervical biopsy and liquid -based cytology test in the screening of cervical lesions .Methods Four hundred and eighty-three patients who udergone the TCT examination from January to December in gynecological clinic were chosen for pathological colposcopy cervical biopsy To select 483 gynecological clinic patients who had the TCT examination and were inspected to be abnormal in Care Hospital from January to December ,2012 ,have pathological colposcopy cervical biopsy for them and retrospective a-nalysis fwas carried out .or their entire clinical datum .Among which the TBS diagnostic system wasis used in the TCT in-spection report ,which contributed to the diagnosis ,as well as combined with the pathological examination report to make the diagnosis.Results Among the patients with the TCT result being ASC-US351,CINⅠ~CINⅢ223 patients(63. 53%) were detected with the pathological colposcopy cervical biopsy;CINⅠ~CINⅢ75 patients(91.46%) were de-tected with the pathological colposcopy cervical biopsy;CINⅠ~CIMⅢ41 patients were detected with the pathological colposcopy cervical biopsy and among the 6 SCC patients ,SCC 6 patients were detected with the colposcopy cervical bi-opsy .Conclusion The diagnose accordance rate of TCT examination and cervical biopsy diagnosis is high ;the diagnose accordance rate of cervical cancer with CIN of higher level is high .Precancerous lesions of uterine cervix can be found at an earlier age with the adoption of colposcopic cervical biopsy combined with TCT examination and stopped or slowed down ,which is the safe ,reliable and accurate examination method for the screening of cervical cancer .%目的:探讨阴道镜宫颈活检与宫颈薄层液基细胞学( TCT )在宫颈病变筛查中的临床应用价值。方法选取2012年1月~12月在妇科门诊行宫颈薄层液基细胞学( TCT)检查异常的483例患者,并同时行阴道镜宫颈活检

  10. Expressions of Toll-like receptors 3, 4, 7, and 9 in cervical lesions and their correlation with HPV16 infection in Uighur women

    Institute of Scientific and Technical Information of China (English)

    Ayshamgu Hasimu; Lin Ge; Qiao-Zhi Li; Rui-Ping Zhang; Xia Guo

    2011-01-01

    Recent findings show that Toil-like receptors (TLRs) expressed in immune cells play a crucial role in the innate immune response and the subsequent induction of adaptive immune responses against microbial infection on tissue injury. Furthermore, expression of TLRs in cancer cells is associated with tumor proliferation and invasion. To explore the role of TLRs expression in cervical carcinogenesis in Uighur women, we detected the expressions of TLR3, TLR4, TLR7, and TLR9 in 25 normal cervical tissues, 64 cervical intraepithelial neoplasia (CIN) tissues, and 63 cervical squamous cell carcinoma (CSCC) tissues using immunohistochemical staining, as well as human papillomavirus type 16 (HPV16) infection using PCR. All samples used in this study were from Xinjiang Uighur women. We found the expression levels of TLR4, TLR7, and TLR9 were significantly higher in CIN and CSCC than in normal controls (P < 0.05). Up-regulation of TLR4 and TLR7 were correlated with tumor differentiation but not FIGO stage or lymph node metastasis (P > 0.05). Up-regulation of TLR9 was correlated with lymph node metastasis (P < 0.05) but not tumor differentiation or FIGO stage (P > 0.05). We also analyzed the correlation between the expressions of TLRs and HPV16 infection and found that the expressions of TLR4 and TLR9 significantly correlated with HPV16 infection in CIN (r = 7.434, P = 0.006; r = 7.123, P = 0.008) and CSCC (r = 6.423, P = 0.001; r = 8.478, P = 0.004), whereas the expression of TLR3 was not significantly different in any of the three groups and had no significant correlation with HPV16 infection. Our results suggest that high expression of TLR4, TLR7, and TLR9 may play important roles in the development and progression of ClN and CSCC in Uighur women, and the expressions of TLR4 and TLR9 can be up-regulated by HPV16 infection.

  11. Screening results on cervical lesions with DNA quantitative cytology and liquid-based cytology%DNA定量细胞学配合液基细胞学对宫颈病变筛查的价值

    Institute of Scientific and Technical Information of China (English)

    曹红英; 武卫华; 许振; 许艳梅

    2011-01-01

    OBJECTIVE: To evaluate the value of the DNA quantitative cytology with liquid-based cytology in cervical cancer prevention and treatment. METHODS: 4 352 cases of patients in our hospital outpatient gynecologic from 01-01-2009 to 10-31-2010 were involved in this study. All the 504 cases which were recommended biopsy were conducted colposcopy and biopsy. The pathological changes were observed after the routine HE staining. Then the positive coincidence rate of the liquid-based cytology was calculated with routine HE staining and DNA Feulgen staining with routine HE staining respectively. RESULTS: The positive rate was 52. 18% (263/504) with the diagnosis of the TBS biopsy criteria. The positive rate was 66.67%(336/504) with the diagnosis of the DNA Feulgen staining biopsy criteria, While the positive rate was 81.75 % (412/504) with the diagnosis of the two combined cytological method biopsy criteria. There was significant difference among the three methods (P<0. 05). CONCLUSIONS: DNA Feulgen staining compared with the single liquid-based cytology, the rate of early detection of cervical lesions is improved significantly. The false negative rate of thc liquid-based cytology can be reduced by the combination of the two, but also the detection rate of cervical lesions of early can be improved. It plays a posive role in order to prevent further development of lesions of early cervical cancer.%目的:评价DNA定量细胞学配合液基细胞学检查在宫颈癌防治中的价值.方法:收集2009-01-01-2010-10-31在我院妇科门诊行液基细胞学及DNA定量检查的患者4 352例,对检查结果建议为活检的病例504例行阴道镜检查及活体组织检查,常规HE染色后观察病变程度,分别计算其与液基细胞学和DNA Feulgen染色后的阳性率.结果:以TBS活检标准行活检病例的阳性检出率为52.18%(263/504),以DNA定量分析结果活检标准行活检的病例阳性检出率为66.67%(336/504),经2种细胞学方法联

  12. 30 CFR 58.610 - Abrasive blasting.

    Science.gov (United States)

    2010-07-01

    ... miners shall use in accordance with 30 CFR 56.5005 or 57.5005 respirators approved for abrasive blasting by NIOSH under 42 CFR part 84, or the operation shall be performed in a totally enclosed device with... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Abrasive blasting. 58.610 Section...

  13. Ultrasonic Abrasive Removal Of EDM Recast

    Science.gov (United States)

    Mandel, Johnny L.; Jacobson, Marlowe S.

    1990-01-01

    Ultrasonic abrasive process removes layer of recast material generated during electrical-discharge machining (EDM) of damper pocket on turbine blade. Form-fitted tool vibrated ultrasonically in damper pocket from which material removed. Vibrations activate abrasive in pocket. Amount of material removed controlled precisely.

  14. Corneal abrasions associated with pepper spray exposure.

    Science.gov (United States)

    Brown, L; Takeuchi, D; Challoner, K

    2000-05-01

    Pepper spray containing oleoresin capsicum is used by law enforcement and the public as a form of nonlethal deterrent. Stimulated by the identification of a case of a corneal abrasion associated with pepper spray exposure, a descriptive retrospective review of a physician-maintained log of patients presenting to a jail ward emergency area over a 3-year period was performed. The objective was to give some quantification to the frequency with which an emergency physician could expect to see corneal abrasions associated with pepper spray exposure. Of 100 cases of pepper spray exposure identified, seven patients had sustained corneal abrasions. We conclude that corneal abrasions are not rare events when patients are exposed to pepper spray and that fluorescein staining and slit lamp or Wood's lamp examination should be performed on all exposed patients in whom corneal abrasions cannot be excluded on clinical grounds. PMID:10830682

  15. Lesões precursoras do câncer cervicouterino: evolução histórica e subsídios para consulta de enfermagem ginecológica Lesiones precursoras del cáncer cervical-útero: evolución histórica consolidando la consulta de enfermería ginecológica Cervical-uterine cancer precursor lesions: historical evolution supporting the gynecological nursing consultation

    Directory of Open Access Journals (Sweden)

    Maria Cristina de Melo Pessanha Carvalho

    2010-09-01

    Full Text Available Estudo emergido de recorte de dissertação de mestrado, ilustrando a evolução histórica das lesões precursoras do câncer cervicouterino (LPCCU. Trata-se da história das LPCCU, delineando a relevância do conhecimento para prática do enfermeiro na área da saúde da mulher. O conceito de LPCCU inicia-se a partir do século XIX, dando início aos estudos das células alteradas. Objetivo: descrever as diversas fases da evolução histórica das alterações cervicais. Estudo qualitativo, descritivo-analítico, recorte temporal no período de 1940 a 2008. Dados levantados mediante bibliografia de fonte primária e recurso BIREME. Pontuaram-se as classificações que já existiram, destacando a Nomenclatura Brasileira, importante para corresponder às necessidades e o perfil da saúde das mulheres do Brasil. Este estudo é o ponto de partida para respaldar as práticas de consulta de enfermagem ginecológica com abordagens educativas, contemplando a população feminina em ações preventivas e incentivo ao tratamento.Estudio emergido de recorte de disertación de máster, ilustrando la evolución histórica de las lesiones precursoras del cáncer cervical-uterino (LPCCU. Se trata de la historia de las LPCCU, delineando la relevancia del conocimiento para la práctica del enfermero en cuidados en la salud de la mujer. El concepto de LPCCU se inicia a partir del siglo XIX, empezando los estudios de las células alteradas. Como objetivo: describir las diversas fases históricas de las alteraciones cervicales. Estudio cualitativo, descriptivo-analítico, recorte temporal en el período de 1940 hasta 2008. Datos acrecentados mediante bibliografía de fuente primaria y recurso BIREME. Se puntuó las diferentes clasificaciones que ya existieron, conde destaque para la nomenclatura brasileña, importante para corresponder a las necesidades y el perfil de la salud de las mujeres del Brasil. Esto estudio es el ponto de partida para respaldar las pr

  16. Utilidad en la combinación de oligonucleótidos universales para la detección del virus del papiloma humano en cáncer cervicouterino y lesiones premalignas Usefulness of combining universal oligonucleotides in detecting human papillomavirus in cervical cancer and premalignant lesions

    Directory of Open Access Journals (Sweden)

    Adela Carrillo

    2004-02-01

    Full Text Available OBJETIVO: Determinar la frecuencia y distribución del virus del papiloma humano en los diferentes estadios que conforman la historia natural del cáncer cérvico uterino, y optimizar la detección mediante el uso de diferentes oligonucleótidos universales. MATERIAL Y MÉTODOS: Se trata de un estudio transversal, descriptivo, en el que las muestras fueron colectadas durante enero a diciembre de 1999. El procesamiento de las muestras y el análisis de los datos se realizaron en el Instituto Nacional de Cancerología en la Ciudad de México. Se hizo análisis comparativo con t de Student para valores continuos y con ji cuadrada para proporciones, y análisis de concordancia entre biopsia y exudado cervical con la prueba estadística de Kappa. Para la detección del virus se utilizó la técnica de la reacción en cadena de la polimerasa (PCR con oligonucleótidos universales los cuales reconocen diferentes regiones del gen L1 (MY09/11; GP5/6; L1C1/2, y oligonucleótidos específicos para el VPH 16 y el VPH 18, así como secuenciación directa de los productos de la PCR. RESULTADOS: Se analizaron 154 muestras: 65 (42.2% citologías normales, 45 (29.2% lesiones de alto y bajo grado, y 44 (28.6% de cáncer invasor. El VPH fue detectado en 95.5% de los casos de cáncer invasor, en 91.6% de lesiones de alto grado, en 66.7% de lesiones de bajo grado y en 23.1% de citologías normales, por la PCR con al menos uno de los juegos de oligonucleótidos utilizados. La detección fue más eficiente en las muestras obtenidas por biopsia que en los exudados cervicovaginales. El porcentaje total de detección del VPH con un juego de oligonucleótidos universales (37.6% aumentó sustancialmente (60.4% al combinarlo con otros dos juegos de oligonucleótidos universales. CONCLUSIONES: La presencia del VPH de alto riesgo es elevada inclusive en mujeres con epitelios cervicales con diagnóstico citológico normal. La detección del VPH mejora al utilizar distintos

  17. HPV prevalence and type-distribution in cervical cancer and premalignant lesions of the cervix: A population-based study from Northern Ireland.

    Science.gov (United States)

    Anderson, Lesley A; O'Rorke, Michael A; Wilson, Robbie; Jamison, Jackie; Gavin, Anna T

    2016-07-01

    Assessment of Human papillomavirus (HPV) prevalence and genotype distribution is important for monitoring the impact of prophylactic HPV vaccination. This study aimed to demonstrate the HPV genotypes predominating in pre-malignant and cervical cancers in Northern Ireland (NI) before the vaccination campaign has effect. Formalin fixed paraffin embedded tissue blocks from 2,303 women aged 16-93 years throughout NI were collated between April 2011 and February 2013. HPV DNA was amplified by PCR and HPV genotyping undertaken using the Roche(®) linear array detection kit. In total, 1,241 out of 1,830 eligible samples (68.0%) tested positive for HPV, with the majority of these [1,181/1,830 (64.5%)] having high-risk (HR) HPV infection; 37.4% were positive for HPV-16 (n = 684) and 5.1% for HPV-18 (n = 93). HPV type-specific prevalence was 48.1%, 65.9%, 81.3%, 92.2%, and 64.3% among cervical intraepithelial neoplasias (CIN) Grades I-III, squamous cell carcinomas (SCC) and adenocarcinoma (AC) cases, respectively. Most SCC cases (81.3%) had only one HPV genotype detected and almost a third (32.0%) of all cervical pathologies were HPV negative including 51.9% of CIN I (n = 283), 34.1% CIN II (n = 145), 18.7% of CIN III (n = 146), 7.8% of SCC (n = 5), and 35.7% of AC (n = 5) cases. This study provides important baseline data for monitoring the effect of HPV vaccination in NI and for comparison with other UK regions. The coverage of other HR-HPV genotypes apart from 16 and 18, including HPV-45, 31, 39, and 52, and the potential for cross protection, should be considered when considering future polyvalent vaccines. J. Med. Virol. 88:1262-1270, 2016. © 2015 Wiley Periodicals, Inc. PMID:26680281

  18. The Distribution and Infection of HPV in Cervical Biopsy Lesions among 450 Women%450例宫颈活检组织中人乳头瘤病毒的分布特征

    Institute of Scientific and Technical Information of China (English)

    王翔; 钱福初; 顾栋桦; 秦基取; 陈瑜; 李雄峰

    2011-01-01

    [Objective] To determine the distribution and infection of HPV in cervical cancer and precancerous lesions. [Methods]Four hundred patients with abnormal cytology were examined by colposcopy. HPV diagnosis was done by polymerase chain reaction using MY09/MY11 primers, for genotyping blot hybridization was used. The pathological results confirmed standards. [Result]A total of 294 women were HPV-DNA positive. HPV-16 was found in 36.7% of all women with positive HPV-DNA,HPV58 in 22. 1%, HPV-18 in 18.7% and HPV-52 in 16. 0%. The infection of HPV in lesions with cervicitis, CIN Ⅰ , CIN Ⅱ and CIN Ⅲ were 55.6 %, 69 %, 82.7 % and 88.9%, respectively. HPV-16,-58,-18 and -52 were found in different cervical lesions. [Conclusions] The finding of HPV-58 and -52 expects HPV-16 and -18 among Huzhou women with CIN Ⅱ and CIN Ⅲ , and has important implications for the development of type-common HPV vaccines.%[目的]了解阴道镜检查患者的宫颈活检组织中HPV的感染率和亚型分布.[方法]对宫颈脱落细胞学异常的患者450例进行阴道镜下定位活检.活检组织同时行HPV基因分型和病理诊断,HPV基因分型采用斑点杂交方法进行.[结果]在450例宫颈活检患者中,其HPV感染率为65.3%.在HPV-DNA阳性患者中,检出率最高的HPV-16占36.7%,依次HPV-58为22.1%,HPV-18为18.7%,HPV-52为16.0%.在宫颈炎、CIN Ⅰ、CIN 、Ⅱ、D,CIN Ⅲ中HPV-DNA阳性率分别为55.6%、69%、82.7%和88.9%.在宫颈炎、CIN Ⅰ、CIN Ⅱ、CIN Ⅲ组织中均检测到HPV-16、-58、-18和-52.[结论]本研究中的宫颈活检组织中不仅检测到HPV-16和-18,还检测到HPV-58和-52感染,后者的发现为开发宫颈癌多价复合型疫苗提供了事实依据.

  19. Life cycle and sustainability of abrasive tools

    CERN Document Server

    Linke, Barbara

    2016-01-01

    This monograph focuses on abrasive tools for grinding, polishing, honing, and lapping operations. The book describes the life cycle of abrasive tools from raw material processing of abrasive grits and bonding, manufacturing of monolithic or multi-layered tools, tool use to tool end-of-life. Moreover, this work highlights sustainability challenges including economic, environmental, social and technological aspects. The target audience primarily comprises research and industry experts in the field of manufacturing, but the book may also be beneficial for graduate students.

  20. A evaluation on cervical intraepithelial lesion and cervical cancer screening by DNA quantitative analysis and liquid-based monolayer cytology%液基薄层细胞学联合DNA定量方法对宫颈病变诊断试验的评价

    Institute of Scientific and Technical Information of China (English)

    侯安丽; 张玉娟; 李秀芬; 邵雪斋; 王杏茶

    2012-01-01

    mistake diagnostic rate,omission diagnostic rate and negative likelihood ratio arc lower than liquid-based monolaycr cytology. The combining sensitivity and the combining specificity of parallel tests between DNA quantitative analysis and liquid-based monolaycr cytology were 99. 56%、44. 52% respectively. The combining sensitivity and the combining specificity of serial tests between the two methods were 83. 78% \\89. 61 %, respectively. The sensitivity of parallel tests is the highest, The specificity of serial tests is the highest. Conclusion Liquid-based monolaycr cytology combined with quantitative DNA analysis may increase the sensitivity and specificity of cervical prccanccrous lesions and early cervical carcinoma.

  1. Cervical cancer: screening, diagnosis and staging.

    Science.gov (United States)

    Tsikouras, Panagiotis; Zervoudis, Stefanos; Manav, Bachar; Tomara, Eirini; Iatrakis, George; Romanidis, Constantinos; Bothou, Anastasia; Galazios, George

    2016-01-01

    Purpose: Despite the widespread screening programs, cervical cancer remains the third most common cancer in developing countries. Based on the implementation of cervical screening programs with the referred adoption of improved screening methods in cervical cytology with the knowledge of the important role of the human papilloma virus (HPV) it's incidence is decreased in the developed world. Even if cervical HPV infection is incredibly common, cervical cancer is relatively rare. Depending on the rarity of invasive disease and the improvement of detection of pre-cancerous lesions due to the participation in screening programs, the goal of screening is to detect the cervical lesions early in order to be treated before cancer is developed. In populations with many preventive screening programs, a decrease in cervical cancer mortality of 50-75% is mentioned over the past 50 years. The preventive examination of vagina and cervix smear, Pap test, and the HPV DNA test are remarkable diagnostic tools according to the American Cancer Association guidelines, in the investigation of asymptomatic women and in the follow up of women after the treatment of pre-invasive cervical cancer. The treatment of cervical cancer is based on the FIGO 2009 cervical cancer staging.

  2. Abrasive Wear of Digger Tooth Steel

    Directory of Open Access Journals (Sweden)

    Hussein Sarhan sarhan, Nofal Al-Araji, Rateb Issa , Mohammad Alia

    2011-08-01

    Full Text Available The influence of silicon carbide SiC abrasive particles of 20, 30, 40, 50 and60μm size on carburized digger tooth steel was studied. Four types of steel, withdifferent hardness, were tested at two constant linear sliding speeds and undervarious loads of 10, 20, 30, 40 and 50N. Tests were carried out for sliding time of0.5, 1.0, 1.5, 2.0 and 2.5min. Experimental results showed that there wasconsistent reduction in abrasive wear as the hardness of the materials wasincreased. It was found that wear increased with the increase of applied load,linear sliding speed and sliding time. Also, it was noticed that the wear increasedwith increase in abrasive particle size, and the most effective size was 40 μm.SEM observations of the worm surface showed that the cutting and ploughingwere the dominant abrasive wear mechanisms.

  3. Cervical myositis ossificans traumatica: a rare location

    Energy Technology Data Exchange (ETDEWEB)

    Baysal, T.; Sarac, K.; Kutlu, R. [Dept. of Radiology, Inonu University, Malatya (Turkey); Baysal, O.; Ersoy, Y. [Dept. of Physical Therapy and Rehabilitation, Inonu Univ., Malatya (Turkey); Elmali, N. [Dept. of Orthopedics and Traumatology, Inonu Univ., Malatya (Turkey)

    1999-05-01

    An unusual case of myositis ossificans traumatica lesion located in the paraspinal region is reported. Despite the contiguity of the lesion with the cervical vertebrae and ominous appearance of the biopsy material, the history of antecedent trauma and computed tomography findings allowed preoperative accurate diagnosis. To our knowledge, myositis ossificans traumatica located in the cervical paraspinal region is very rare. (orig.) With 4 figs., 16 refs.

  4. 5-type HPV mRNA versus 14-type HPV DNA test: test performance, over-diagnosis and overtreatment in triage of women with minor cervical lesions

    OpenAIRE

    Westre, Bjørn; Giske, Anita; Guttormsen, Hilde; Sørbye, Sveinung Wergeland; Skjeldestad, Finn Egil

    2016-01-01

    Background Repeat cytology and HPV testing is used in triage of women with minor cytological lesions. The objective of this study was to evaluate 14-type HPV DNA and 5-type HPV mRNA testing in delayed triage of women with ASC-US/LSIL. Methods We compared a DNA test (Roche Cobas 4800) and an 5-type mRNA test (PreTect HPV-Proofer). In total 564 women were included in the study. Results The sensitivity among solved cases for CIN3+ were 100 % (15/15) for both tests. The sensitivity for CIN2+ of t...

  5. Expresi??n de ant??genos HLA clase I e infecci??n por papilomavirus humano tipo 16 en la neoplasia cervical uterina y lesiones precursoras

    OpenAIRE

    Torres Garc??a, Luis Manuel

    1992-01-01

    Sobre una casu??stica de 109 diagn??sticos de diferentes entidades nosologicas de cervix uterino, se determina la presencia de ant??genos hla clase i mediante la t??cnica de la inmunoperoxidasa, encontrando que tanto el epitelio normal como los condilomas y las lesiones intraepiteliales conservan la expresi??n de estos ant??genos, mientras que los tumores invasores pierden esta capacidad en el 15% de los casos, correlacion??ndose con la invasi??n del estroma y un aumento de su malignidad. Se ...

  6. The measurement of abrasive particles velocities in the process of abrasive water jet generation

    Science.gov (United States)

    Zeleňák, Michal; Foldyna, Josef; Říha, Zdeněk

    2014-08-01

    An optimization of the design of the abrasive cutting head using the numerical simulation requires gathering as much information about processes occurring in the cutting head as possible. Detailed knowledge of velocities of abrasive particles in the process of abrasive water jet generation is vital for the verification of the numerical model. A method of measurement of abrasive particles at the exit of focusing tube using the FPIV technique was proposed and preliminary tests are described in the paper. Results of analysis of measured velocity fields are presented in the paper.

  7. Impact-abrasion and abrasion of WC-Co: wear mechanisms in severe environments

    Energy Technology Data Exchange (ETDEWEB)

    Hawk, Jeffrey A.; Wilson, Rick D.; Osara, K. (Outokumpu Research Oy)

    2003-10-01

    Hard and super-hard materials have very good abrasive wear resistance. However, in many severe wear environments that make use of these materials, impact is a significant component of that environment. Consequently, the behavior of many of these hard materials in impact-wear conditions need to be understood with respect to the mechanisms of material removal such small scale fracture and subsequent crack growth in the carbide. This study details the behavior of several ?hard? materials in abrasion and impact-abrasion focusing on the mechanisms of material removal due to impact and abrasion.

  8. Performance of recycling abrasives in rock cutting by abrasive water jet

    Institute of Scientific and Technical Information of China (English)

    Gokhan Aydin

    2015-01-01

    Rock cutting performance of recycling abrasives was investigated in terms of cutting depth, kerf width, kerf taper angle and surface roughness. Gravity separation technique was employed to separate the abrasives and the rock particles. The recycling abrasive particles were then dried and sieved for determination of their disintegration behaviors. Before each cutting with recycling abrasives, the abrasive particles less than 106mm were screened out. It is revealed that a considerable amount of used abrasives can be effectively reused in the rock cutting. The reusabilities of abrasives are determined as 81.77%, 57.50%, 34.37%and 17.72%after the first, second, third and fourth cuttings, respectively. Additionally, it is determined that recycling must be restricted three times due to the excessive disintegration of abrasives with further recycling. Moreover, it is concluded that cutting depth, kerf width and surface roughness decreases with recycling. No clear trend is found between the kerf taper angle and recycling. Particle size distribution is determined as an important parameter for improving the cutting performance of recycling abrasives.

  9. Abrasive water jet: a complementary tool

    OpenAIRE

    Duarte, J. P.; Peças, P.; E NUNES; H. Gouveia

    1998-01-01

    The abrasive water jet is a powerful cutting tool, whose main advantages lie in the absence of thermal effects and the capability of cutting highly thick materials. Compared with Laser, the abrasive water jet allows the cutting of a larger range of thicknesses and a wider variety of materials such as: ornamental stones, metals, polymers, composites, wood, glass and ceramics. The application of this technology has suffered an extensive growth, with successful applications in varied industrial ...

  10. Third abrasive wear mode: is it possible?

    Directory of Open Access Journals (Sweden)

    Ronaldo Câmara Cozza

    2014-04-01

    Full Text Available The objective of this paper is to propose an initial discussion on the characterization of a third abrasive wear mode. The results obtained in a previous work [1] under different test conditions revealed the occurrence of the superposition of the “rolling” and “grooving” abrasive wear modes. This phenomenon was denoted “micro-rolling abrasion” due to the observation that “rolling abrasion” was found to act on “grooving abrasion”.

  11. The role of HPV genotyping test and liquid-based cytology in diagnosis of cervical pre-cancerous lesions%人乳头瘤病毒分型基因检测和液基细胞学检查在宫颈癌前病变中的诊断作用

    Institute of Scientific and Technical Information of China (English)

    谢芳; 黎云; 彭秀红; 李洁明

    2011-01-01

    Objective To evaluate the role and significance of HPV genotyping detection and Thinprep cytology test (TCT ) in the diagnosis of cervical precancerous lesions ( mild to moderate epithelial neoplasia ). Methods The findings of HPV genotyping and TCT were analyzed in 401 patients with cervical precancerous lesions ( CIN1-CIN2 ) who had been hospitalized between November 2008 to November 2010. Results The sensitivity of HPV genotyping was significantly higher than that of TCT ( P<0.01 ).TCT combined with HPV genotyping enhanced the detection rate of cervical lesions. Subtypes 16, 52, 58, and 18 of HPV were common in high-risk HPV infection with cervical precancerous lesions. Subtype 16 had a markedly higher rate of occurrence of cervical lesions than subtypes 52 and 58 ( P < 0.01 ). Conclusions High-risk HPV infection is the major pathogenic factor for cervical cancer and precancerous lesions. HPV 16 infection easily leads to cervical lesion. HPV genotyping test combined with Thinprep cytology test can increase the detection rate of cervical precancerous lesions.%目的 评估人乳头瘤病毒(HPV)基因分型检测和液基细胞学检查(TCT)在宫颈轻、中度上皮细胞内瘤变(CIN1-CIN2)中的诊断作用及意义.方法 对我院2008年11月-2010年11月期间401例因宫颈癌前病变(CIN1-CIN2)要求官颈环切的患者的HPV分型基因检测情况与液基细胞学检查结果进行总结分析.结果 HPV的灵敏性较TCT明显增高(P<0.01),TCT和HPV基因分型检测结合能提高宫颈病变的检出率;宫颈癌前病变中HPV感染以高危基因组的16、52、58、18亚型感染多见,HPV16犁感染引起高度病变的几率较HPV52型和HPV58型明显增高(P<0.01).结论 高危HPV感染是宫颈癌及癌前病变发病的主要致病因子,HPV16型感染易导致宫颈高度病变;HPV基因分型检测和液基细胞学检查结合,可以提高宫颈癌前病变的检出率.

  12. Liquid-based Cytology with Colposcopy in the Clinical Diagnostic Application of Cervical Lesions%液基细胞学联合阴道镜检诊断宫颈病变

    Institute of Scientific and Technical Information of China (English)

    陈宁静; 涂干卿

    2010-01-01

    目的 研究液基薄层细胞学(TCT)检查联合阴道镜检对宫颈病变诊断的价值.方法 选择1 407例患者进行TCT、阴道镜检查和活组织病理检查.结果 281例单独行阴道镜检和699例单独行TCT检查提示活检的,分别有39.50%和42.63%属CIN Ⅰ级以上病变;427例TCT检查结合阴道镜检提示活检的,有86.42%属CIN Ⅰ级以上病变.结论 TCT检查结合阴道镜检定位活检可提高宫颈病变的准确诊断率.%Objective Studying the clinical diagnostic value of liquid-based thin-layer cytology(TCT)combined colpoacopic examination for cervical lesions.Methods In May 2006~August 2007 choose the 1407 patients coming the hospital who was done cytology examination(TCT)and coiposcopy examination and biopsy.Results Among 281 cases of eolposcopy prompt biopsy 39.50% of them suffered from CIN Ⅰ.So did 42.63 % of 699 cases of TCT examinations.427 cases of TCT combined Colposcopy were prompted they need to do biopsy.86.42% of them suffered from CIN Ⅰ or above lesions.Conclusion TCT combination of colposcopy and endoscopic biopsy can increase the rate of accurate diagnosis of cervicallesions.

  13. Cervical radiculopathy.

    Science.gov (United States)

    Iyer, Sravisht; Kim, Han Jo

    2016-09-01

    Cervical radiculopathy is a common clinical scenario. Patients with radiculopathy typically present with neck pain, arm pain, or both. We review the epidemiology of cervical radiculopathy and discuss the diagnosis of this condition. This includes an overview of the pertinent findings on the patient history and physical examination. We also discuss relevant clinical syndromes that must be considered in the differential diagnosis including peripheral nerve entrapment syndromes and shoulder pathology. The natural history of cervical radiculopathy is reviewed and options for management are discussed. These options include conservative management, non-operative modalities such as physical therapy, steroid injections, and operative intervention. While the exact indications for surgical intervention have not yet been elucidated, we provide an overview of the available literature regarding indications and discuss the timing of intervention. The surgical outcomes of anterior cervical decompression and fusion (ACDF), cervical disc arthroplasty (CDA), and posterior cervical foraminotomy (PCF) are discussed. PMID:27250042

  14. The effect of aging of formalin-fixed paraffin-embedded tissues on the in situ hybridization and immunohistochemistry signals in cervical lesions.

    Science.gov (United States)

    Nuovo, Allison J; Garofalo, Michela; Mikhail, Alexandria; Nicol, Alcina F; Vianna-Andrade, Cecilia; Nuovo, Gerard J

    2013-09-01

    Formalin-fixed, paraffin-embedded tissues are widely used in biomedical research but little is known about the effect of the age of the block or unstained slides on the in situ hybridization or immunohistochemistry signal. We compared the in situ-based and immunohistochemistry-based signals for cervical intraepithelial neoplasia samples that ranged from 0 to 15 years of age. There was a progressive and statistically significant decrease in the strength of the p16 signal when comparing tissues prepared from recent unstained slides (0 to 1 y old, mean score of 92%) to those of intermediate age (5 to 7 y old, mean score of 49%) to old unstained slides (cut 13 to 15 y ago, mean score of 10%). Equivalent, progressive, and significant decreases in the intensity of the signals for microRNAs, CD45, and human papillomavirus DNA were seen in tissues stored on slides from 5 to 7 years and 13 to 15 years, respectively. However, the diminution of signal was much less, although still statistically significant, if the sections from the 13- to 15-year-old paraffin blocks were prepared in 2012. The data likely does not represent degradation of the targets as extraction of several microRNA from the old blocks showed no detectable degradation, despite the markedly weakened in situ hybridization signal. It is concluded that in situ-based signal for DNA, microRNAs, and proteins in paraffin-embedded tissues are significantly reduced over time, especially when stored long term on glass slides which, in turn, can lead to a significant underestimation of the amount and presence of the nucleic acid or protein target.

  15. 液基细胞结合阴道镜下活钳在宫颈病变中的应用%Application of Liquid Based Cytology Combined with Colposcopy Biopsy in Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    税锦华; 李晓冬

    2014-01-01

    目的:了解液基细胞结合阴道镜下活钳对诊断宫颈病变的临床价值。方法对我院2010年1月1日至2013年12月31日妇科门诊自愿作TCT的6718例已婚妇女作TCT检测,结果异常的再作阴道镜活钳。TCT检查:用特制的塑料刷在宫颈内口磷柱状上皮交界处旋转6~8周,放入液基细胞保存液瓶中,经自动制片机制成簿片,经巴氏染色封片,由专业病检医生读片。阴道镜检查由专业从事阴道镜的医生,在子宫颈转化区3、6、9、12点处活钳或采用醋酸涂宫颈在醋酸白上皮区取材行宫颈活钳。结果⑴TCT筛查6718例异常的573例,占8.57%。⑵阴道镜下活钳573例病理结果:炎症201例,CINⅠ287例,CINIⅡ53例,CINⅢ24例,SCC8例。结论液基细胞学结合阴道镜活检对宫颈病变可提高阳性诊断率,做到早期诊断、早期治疗。%Objective To understand the clinical value of the liquid based cytology combined with colposcopy biopsy in the diagnosis of cervical lesions.Methods 6718 married women who were voluntary to test by TCT were selected by the out-patient gynecological department of our hospital from January 1, 2010 to December 31, 2013, while the abnormal one was tested by further colposcopy biopsy. TCT examination: the special plastic brush was used to rotate 6 - 8 weeks in the junction of endocervix phosphorus columnar epithelial cell, then the cells were put into basal cell preservation liquid bottles. The slices were made by automatic production machine and stained by Pap stain and mounting. The pathological specimens were checked by professional doctor in under the microscope. Colposcopy operation was performed by specializing colposcopy doctors in 3, 6, 9, 12 at the cervical transformation zone given or smearing acetate on cervix then given cervicalbiopsy on acetowhite epithelium area.Results (1) The screening of TCT in 6718 cases of abnormal 573, accounted for 8.57%.(2) The results of 573

  16. HPV-DNA和TCT检测在宫颈癌前病变筛查中的诊断价值%Diagnostic Value of HPV-DNA and TCT Test in Screening of Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    张汉园; 沈丽萍; 曹风华; 施瑜; 盈丽霞

    2012-01-01

    目的:探讨乳头瘤病毒定性检测( HPV- DNA)与液基薄层细胞学检查技术(TCT)在宫颈癌前病变中的诊断价值.方法:分别采用HPV- DNA及TCT法检测1536例患者,对TCT阳性、HPV- DNA阳性病例者进行阴道镜下活检.结果:随着病理诊断级别的升高,高危型HPV感染率上升,而低危型HPV感染多见于轻度不典型增生(CINⅠ)及其以下病变,HPV混合感染在各组间未见明显趋势.HPV-DNA检测与宫颈活检的符合率为66.78%.随着病理级别的升高,不典型鳞状细胞(ASCUS)组的感染率呈降低趋势,鳞状上皮内低度病变(LSIL)组感染率亦呈下降趋势,而鳞状上皮内高度病变(HSIL)组和鳞癌(SCC)组感染率均呈上升趋势,TCT检测与组织学诊断的符合率为66.67%,与HPV-DNA检查比较,差异无统计学意义(x=0.001,P>0.05).结论:HPV-DNA、TCT及宫颈活检三者检测相结合,能明显提高诊断的正确性.%Objective: To study the clinical application value of the qualitative detection of human papillomavirus (HPV-DNA) and ThinPrep cytology technique (TCT) in prevention of cervical lesions and cervical cancer. Methods: Totally 1536 cases were screened by HPV-DNA test or TCT test. Directed biopsies under colposcopy were taken either in both positive test. Results: The pathological diagnosis level rose when high-risk HPV infection increased, while low-risk HPV infection was appeared more commonly in CIN I lesions, but HPV co-infection had no significant trend in each group. The compliance rate of HPV-DNA testing with the histological diagnosis was 66.78%. With the increased pathological grade, ASCUS group infection rate was decreasing, and LSIL group infection rate was also found a downward trend, while the prevalence of HSIL group and SCC group were rising. The compliance rate of TCT test with the histological diagnosis was 66.67 %, and there was no significantly difference between HPV-DNA test and TCT test (X2 = 0.001, P> 0.05). Conclusion: The

  17. Study of human papillomavirus infection and cervical intraepithelial lesion in HIV/AIDS%HIV/AIDS与人乳头瘤病毒感染及宫颈病变的相关性研究

    Institute of Scientific and Technical Information of China (English)

    郭晓峰; 李晶; 戴卫东

    2013-01-01

    Objective:To determine the characteristics of high-risk type of human papillomavirus (HR-HPV)infection and cervical lesion in HIV/AIDS patients.To provide clues and evidence for the prevention and treatment of cervical cancer in HIV/AIDS patients.Methods:166 HIV/AIDS patients and 476 non-HIV patients from Apr.2009 to Jul.2012 were analyzed to compare the results of HR-HPV and TCT test.Results:The infection ratio of HIV (+) group was significant higher than HIV (-) group.20 cases of HIV (+) group received cervical biopsy,in which 13 cases (65.00%) were diagnosed above CIN Ⅰ level.21 cases of HIV(-) received cervical biopsy,in which 19 cases (90.48%) were proven above CIN Ⅰ level.Among HIV patients,there were significant differences in the number of patients with CD4+ less than 200cell/ul between HR-HVP (+) and HR-HPV (-) patients (P=0.030).Conclusions:The risk of HR-HPV infection in HIV/AIDS patients is much higher than that of non-HIV infected patients.Continuous observation should be conducted on HIV-HPV co-infected patients.The existence of HR-HPV infection may depends on functions of immune svstem.%目的:探讨艾滋病病毒感染者/艾滋病患者(HIV/AIDS)生殖道高危型人乳头瘤病毒(HR-HPV)感染及宫颈病变的特点,为HIV/AIDS患者宫颈癌的防治研究提供线索和依据.方法:选取2009年4月至2012年7月就诊于我院的166例HIV/AIDS患者[HIV(+)组]与476例非HIV感染患者[HIV(-)组],比较两组患者的HR-HPV感染率及液基细胞学检测(TCT)检测结果.结果:HIV(+)组的HR-HPV感染率(38.55%,64/166)显著高于HIV(-)组(10.50%,50/476)(P<0.001).HIV(+)组中20例行宫颈活检,其中13例(65.00%)发生CIN Ⅰ以上病变;HIV(-)组中21例行宫颈活检,19例(90.48%)发生CIN Ⅰ以上病变.HIV(+)组中HR-HPV(+)与HR-HPV(-)者的CD4<200cell/μl的比率分别为29.29%和17.48%,差异显著(P=0.03).结论:HIV/AIDS患者感染HR-HPV的风险显著高于非HIV感染者,对HIV-HPV联合感染

  18. Evaluation of genomic amplification of the human telomerase RNA component gene in the screening of cervical lesions%人端粒酶RNA基因检测在子宫颈病变筛查中的意义

    Institute of Scientific and Technical Information of China (English)

    江静; 魏丽惠; 屠铮; 张果; 李静然; 赵丽君; 赵超; 崔淑慧; 李小平; CHEN Zhong

    2008-01-01

    目的 探讨人端粒酶RNA(hTERC)基因检测在宫颈病变筛查中的意义.方法 选择经宫颈液基细胞学检查为正常一高度鳞状上皮内瘤变(HSIL)的301例患者为研究对象,采用人乳头状瘤病毒(HPV)杂交捕获2代(HC2)方法检测其高危型HPV感染状况,病理学检查明确其病变性质,荧光原位杂交(visa)技术检测其hTERC基因异常扩增情况.以病理学结果为金标准,将FISH技术检测结果与液基细胞学和HC2方法检测结果进行比较.结果 301例患者中,宫颈液基细胞学检查为正常、不典型鳞状细胞(ASC)、低度鳞状上皮内瘤变(LSIL)与HSIL细胞中,hTERC基因异常扩增率分别为3.0%(6/203)、21.2%(14/66)、44.4%(8/18)和92.9%(13/14),两两比较,差异均有统计学意义(P0.05);而特异度前者明显高于后者(分别为67.8%~73.5%和25.6%~27.7%,P0.05),4:4型比例明显升高(P<0.01).结论 应用FISH技术检测hTERC基因异常扩增情况可辅助液基细胞学检查和HPV HC2方法诊断高级别CIN;且hTERC基因异常扩增信号为2:4和4:4型以上可能是进展为高级别CIN的预测指标.%Objective To investigate the genomic amplification of the human telomerase RNA component (hTERC) gene in cervical cytology and evaluate its role in screening of cervical lesions. Methods A total of 301 cases were recruited, with liquid-based cytology diaghoses as normal (n=203), atypical squamous cells (ASC, n=66), low-grade squamous intraepithelial lesions ( LSIL,n=18), and high-grade squamous intraepithelial lesions ( HSIL, n=14). Following cytological examination, the slides were analyzed using a two-color fluorescence in aitu hybridization ( FISH ) probe targeted to chromosome 3q26 containing hTERC. The hTERC findings were compared to the cytologic and histologie results, as well as high-risk human papilloma viruses (HPV) results. Results Genomie amplification of hTERC was found in 3.0% (6/203)of normal specimens, 21.2% (14/66) of ASC, 44.4% (8/18) of

  19. Abrasive Blasting Unit (ABU) - 16270

    International Nuclear Information System (INIS)

    NUKEM Technologies was contracted to supply a dry, automated drum belt (tumbling) Abrasive Blasting Unit (ABU) to the Joint Research Centre of the European Commission in Ispra, Italy. The ABU was installed in the centralised radioactive waste management area of the JRC-Ispra site in Italy. The unit is to be employed for the decontamination to clearance levels of slightly contaminated metal components and, where practical, concrete or heavy concrete (density ∼3200 kg/m3) blocks arising from the dismantling of nuclear facilities. The presentation is based on the successful construction and installation of the ABU at the JRC Ispra site. Among the several possibilities of adapting conventional abrasive units to nuclear applications, an automatic tumbling machine was preferred, due to the larger output and (mainly) for the ease of operation, with minimum direct handling of contaminated material by operators, thus satisfying the ALARA principle. Consideration was also given to Belgoprocess' successful experience with a predecessor, similar unit. After adequate size reduction batches of up to about 800 kg of material to be decontaminated are automatically introduced into the blasting chamber. Pieces between 100 mm and 800 mm long, between 100 mm and 500 mm wide and between 5 mm and 300 mm high can be effectively treated in the unit, the maximum weight of a single piece being limited to 100 kg. Short lengths of pipe may be included; the final dimensions of pipe to be decontaminated will be established during the nuclear commissioning tests. Other components with hard-to-reach surfaces may also be included. The content of the chamber is tumbled by two bladed drums, while sharp steel grit is sprayed onto the contaminated components, thus removing the surface layer including any contamination. From experience, 30 minutes of treatment is sufficient to remove contamination to levels below expected clearance levels for most materials. The decontaminated components are removed

  20. Clinical value of high-risk human papillomavirus DNA test,liquid-based cytology test in diagnosis of cervical cancer and precancerous lesion%高危型人乳头状瘤病毒DNA、液基薄层细胞检测在宫颈癌及前期病变诊断中的临床价值

    Institute of Scientific and Technical Information of China (English)

    杨秀珍

    2014-01-01

    Objective To investigate the clinical significance of high-risk human papillomavirus (HPV) DNA test com-bined with cytology inspection in the screening of cervical precancerous lesions. Methods 5080 women were selected from February 2013 to January 2014 in our hospital,they were given screen of cervical precancerous lesions.Cervical cytology,high-risk HPV DNA testing,colposcopy and cervical biopsy were done in them,and the results were analyzed with statistical methods. Results ASC-US (atypical squamous meaning is not clear epithelial cells) group of 318 cases, LSIL (low-grade squamous intraepithelial lesions) 26 cases of group,HSIL (high-grade squamous intraepithelial lesion) group of 52 cases,6 cases of invasive group confirmed by histopathological examination. Sensitivity and negative predic-tive value of HPV +TCT (liquid-based cytology test) to detect cervical lesions were higher than those high-risk HPV DNA and TCT,the specificity of TCT detection of cervical lesions was higher than high-risk HPV DNA and HPV DNA+ TCT,the difference was significant (P<0.05). Conclusion The sensitivity and negative predictive value of high-risk HPV DNA testing combined with cytology testing in the cervical cancer screening are higher,and has positive im-plications for screening for cervical lesions.%目的:探讨高危型人乳头状瘤病毒(HPV)DNA检测联合宫颈细胞学检查对宫颈癌前病变筛查的临床意义。方法5080名女性均在2013年2月~2014年1月选择在本院就诊并进行宫颈癌前病变筛查,对其进行宫颈细胞学检查、高危型HPV DNA检测、阴道镜下行宫颈活检,使用统计学方法分析检测结果。结果经组织病理检查确诊ASC-US(不能明确意义的非典型鳞状上皮细胞)组318例,LSIL(低度鳞状上皮内病变)组26例,HSIL(高度鳞状上皮内病变)组52例,浸润癌组6例,HPV DNA+液基薄层细胞检测(TCT)检测宫颈病变的灵敏度及阴性预测值高于高危型HPV DNA检测及TCT,TCT

  1. Influência da adequabilidade da amostra sobre a detecção das lesões precursoras do câncer cervical Influence of adequacy of the sample on detection of the precursor lesions of the cervical cancer

    Directory of Open Access Journals (Sweden)

    Rita Goreti Amaral

    2008-11-01

    Full Text Available OBJETIVO: avaliar se a adequabilidade da amostra influencia na detecção das lesões precursoras do câncer do colo do útero. MÉTODOS: este foi um estudo de corte transversal, realizado no período de Janeiro de 2004 a Dezembro de 2005. Foram incluídos 10.951 resultados de exames citopatológicos cervicais, tendo como base usuárias do Sistema Único de Saúde de Goiânia, Goiás. Essas mulheres procuraram, espontaneamente, os serviços do Programa Saúde da Família ou Unidades Básicas de Saúde. A coleta foi feita por médicos e enfermeiros por meio da técnica convencional para rastreamento de câncer do colo do útero. Os esfregaços analisados foram classificados de acordo com o Sistema Bethesda, sendo a adequabilidade da amostra definida durante o escrutínio de rotina e categorizada como: satisfatória; satisfatória, porém apresentando fatores que prejudicam parcialmente a análise; e insatisfatória. Os resultados obtidos foram armazenados no programa Epi-Info 3.3.2. Para a comparação entre os resultados alterados e a adequabilidade da amostra dos esfregaços citopatológicos utilizou-se o teste do χ2. Foram consideradas significantes as diferenças em que a probabilidade de rejeição da hipótese de nulidade foi menor que 5% (pPURPOSE: to evaluate whether the sample adequacy influences the detection of precursor cervical cancer lesions. METHODS: a transversal study from January 2004 to December 2005. A number of 10,951 results of cervical cytotopathological exams from users of the National Health System (Sistema Único de Saúde, SUS in Goiânia, Goiás , Brazil, was studied. These women had spontaneously looked for the services from the Family Health Program or from the Basic Units of Health. Samples were collected by medical doctors and nurses, through the conventional technique to detect cervical cancer. The analyzed smears were classified by the Bethesda System, the sample adequacy being defined along the routine

  2. 宫颈 LCT结合阴道镜及宫颈活检对宫颈上皮内瘤变和宫颈癌患者的早期诊断价值研究%Application Value of the LCT Combined with Tissue Biopsy in Early Screening for Cervical Epithelial Lesions and Cervical Cancer

    Institute of Scientific and Technical Information of China (English)

    洪恩

    2014-01-01

    目的:探讨宫颈液基细胞学检查( liquid-based cytological test ,LCT)结合阴道镜及宫颈活检对宫颈上皮内瘤变和宫颈癌患者的早期诊断价值。方法回顾性分析250例宫颈LCT阳性的病例,对其进行宫颈活检。比较细胞学与组织学结果,并研究发病率与年龄的关系。结果在所有LCT阳性病例中,ASC-US 142例(在阳性病例中占56.8%), AGC 为3例(1.2%),ASC-H 24例(9.6%),LSIL 82例(54.4%),HSIL为26例(10.4%),SCC 1例(0.4%);宫颈活检结果中,炎性反应66例(26.4%),挖空细胞25例(10.0%),CINⅠ级54例(21.6%),CINⅡ级59例(23.6%),CIN Ⅲ级28例(11.2%),鳞癌16例(6.4%),腺癌2例(0.8%),以组织学结果验证细胞学结果,符合率分别为 ASC-US (100.0%),ASC-H(92.3%),LSIL(63.2%),HSIL(72.9%),SCC(100.0%);并且宫颈病变多见于30~50岁年龄段。结论宫颈LCT 具有较高的准确性,是筛查宫颈上皮性病变的重要手段,结合阴道镜及宫颈活检可使宫颈上皮内瘤变和宫颈癌患者得到早期诊断和早期治疗。%Objective To explore the application value of the LCT combined with tissue biopsy in early screening for cervical epithelial lesions and cervical cancer .Methods 50 cases of abnormal cytology received cervical LCT inspection were retrospectively analyzed and were treated with biopsy inspection .The cytology and histology results were compared ,and the rela-tionship of lesion and age was analyzed .Results Among those abnormal cytology,the ASC-US was 142 cases (56.8%),AGC was 3 cases(1.2%),ASC-H was 24 cases (9.6%),LSIL was 82 cases (54.4%),HSIL was 26 cases (10.4%),and SCC was 1 cases (0.4%).Among the 250 cases of biopsy,inflammation reaction were 66 cases (26.4%),koilocyte 25 cases (10.0%), CINⅠ54 cases (21.6%),CINⅡ59 cases (23.6%),CINⅢ28 cases

  3. Application of human papillomavirus genotyping detection and thinprep cytology test in screening of cervical lesions%HPV分型检测和TCT检查在宫颈病变筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    卜祥茂; 徐莉莉; 陈艳萍; 宋卫青

    2014-01-01

    Objective:To evaluate the role of human papillomavirus(HPV) genotyping detection and thinprep cytology test(TCT )for screening cervical lesions.Methods:A retrospective analysis 1128 cases of cervical cytology specimens from September 2012 to September 2013 using HPV、TCT and cervical biopsy.Results: In 1128 cases of specimens, the positive rate for HPV infection was 64.4%(726/1128) and there were 21 kinds of genotypes, high-risk subtype 16 kinds, low-risk types 5 kinds. High-risk subtype infection were 16 (25.0%), 58 (12.1%), 52(10.2%) and low-risk subtype main was HPV 61(3.4%), 11(1.9%). Single subtype was detected in 555 cases,double or multiple 171 cases, and quadruple infection was also can be detected. TCT ≥ASC-US was 87.8%(990/1128). In ASCUS, LSIL, HSIL and SCC, the detection rates of HPV infection were 60.3 %, 71.8%, 80.3%,100 % respectively. The positive predict value (PPV) of HPV genotype detection was 74.1% and negative predict value(NPV) was 68.7%. The PPV and NPV of TCT were 63.2% and 72.5%, and HPV joint TCT were 75.5% and 100%. The PPV of group H and group H+T was higher than that of group T. The NPV of group H+T were higher than that of group H and group T.Conclusions:The genotyping technology for detection HPV has very high accuracy, joint TCT screen diseased cells effectively in cervical lesions, and can provide more reliable scientific basis for the prevention and treatment of cervical cancer and for the use of HPV vaccine in Qingdao.%目的:探讨人乳头瘤病毒(HPV)分型检测和液基细胞学(TCT)检查在筛查宫颈病变中的应用。方法:回顾性分析2012.9~2013.9在我院妇科门诊就诊,同时采用HPV分型和TCT检查的门诊妇女宫颈脱落细胞标本1128例,并最终进行组织病理学检查。结果:1128例标本中HPV阳性率为64.4%(726/1128),共检出21种亚型,高危亚型16种,低危亚型5种,高危型以16,58,52多见,构成比分别为25.0%,12.1%和10.2%。低危型以61,11型

  4. The clinical significance of TCT joint the high-risk HPV-DNA detection in cervical lesions screening%液基细胞学与高危型HPV-DNA联合检测在宫颈病变筛查中的意义

    Institute of Scientific and Technical Information of China (English)

    喻垚; 郭变琴; 罗光丽; 赖年钰

    2015-01-01

    Objective To study the clinical significance of TCT and the high-risk HPV-DNA detection in cer-vical lesions screening .Methods There were 386 cases of cervical cancer ,555 cases of clinical cervicitis ,269 cases of CINⅠ ,133 cases of CINⅡ /Ⅲ ,all have been confirmed by histopathological diagnosis .We detected the cytology le-sions through Thinprep cytology test (TCT ) ,and detected the expression of high-risk HPV-DNA through second-generation hybrid capture Ⅱ(hybrid captme ,HC Ⅱ) .Results The positive expression rates of high-risk HPV-DNA in cervicitis ,CINⅠ ,CINⅡ /Ⅲ ,cervical cancer w ere 26 .5% ,48 .7% ,54 .9% ,72 .0% ,and there w ere statistically sig-nificance in the four groups (P<0 .01) .In screening CINⅠ or above ,sensitivity ,specificity ,PPV and NPV of HR-HPV detection combined with cytology were 80 .2% ,88 .5% ,86 .7% ,81 .3% ,and higher than the separate detec-tion .Conclusion Infection rate of HR-HPV is ascending with serious degree of cervical lesion .HR-HPV detection by HC-Ⅱ is an important method in screening cervical lesion .Combined TCT and HR-HPV test will improve the detec-tion rate of cervical lesions;it is an ideal method to screening cervical cancer and prevention .%目的 探讨薄层液基细胞学(TCT)与高危型人乳头状瘤病毒(HR-HPV) DNA检测在宫颈病变筛查中的临床意义.方法 2012年1月至2014年1月该院就诊的宫颈病变患者1 343例 ,其中临床已病理确诊的宫颈癌386例 ,宫颈炎555例 ,CINⅠ269例 ,CINⅡ /Ⅲ 133例 ,对所有患者均采用 TCT 法检测细胞病变 ,同时使用第2代杂交捕获法(HC2)检测高危型 HPV-DNA的阳性表达率 ,并比较2种方法的敏感性、特异性、阳性预测值和阴性预测值.结果 高危型 HPV-DNA 在宫颈炎、CIN Ⅰ 、CIN Ⅱ /Ⅲ 和宫颈癌中的阳性率分别为 26 .5% 、48 .7% 、54 .9% 、72 .0% ,4组患者之间比较 ,差异有统计学意义(P<0 .01);TCT与高危型HPV-DNA联合检测其敏感性为80 .2

  5. 高危HPV病毒第2代杂交捕获法检测应用于宫颈癌以及癌前病变检查%Application of Hybrid Capture 2 for High-Risk Human Papillomavirus Testing in Cervical Cancer and Premalignant Lesion

    Institute of Scientific and Technical Information of China (English)

    张玉梅; 曹维克

    2011-01-01

    Objective: To explore the clinical value of hybrid capture 2 (hc2) in detection of high risk HPV in cervical cancer and pre-cancerous cervical lesions. Methods: Cervix exfoliated cells were detected with the second generation of hybrid capture hc2 method, and the diagnostic results such as specificity, sensitivity, accuracy, and pedicted value were compared with that of pathological diagnosis respectively. Results: The infection rate of HPV assayed by hc2 was 43. 8% in atypical squamous cell of undetermined signification (ASCUS), 7. 8% in ASCUS-H, 9. 7% low-grade squamous intraepithelial lesion(LSIL), and 28. 7% in high-grade squamous intraepithelial lesion (HSIL) respectively according to TBS diagnosis and classification system. For high grade-squ-mous intraepithelial lesion≥(CIN II ), the specificity, sensitivity, positive-predictive value and negative-predictive value by hc2 were 66. 7% , 93. 4% , 60. 0% , and 95. 0% respectively. In high virus load assay, there was significant difference between cervical cancer and CIN I (P<0. 01), and between cervical cancer/CIN and NILM group (P<0. 01), but there was no difference be-tween CIN and cervical cancer. Conclusion; The high-risk HPV test with hc2 is still a good method in diagnosing cervical cancer and premalignant lesion. Examining the high-risk HPV viral load with hc2 is related to cervical cancer and premalignant lesions, but not related to the severity of cervical lesions.%目的:探讨高危人类乳头瘤病毒(HPV)第二代杂交捕获实验(hc2)检测应用于宫颈癌以及癌前病变检查中的临床价值.方法:用hc2检测其子宫颈脱落细胞,以病理诊断作为金标准对检测结果进行分析和评价,对其特异度、敏感度、准确性、预测值等检测并分析.结果:hc2法检测高危型HPV的感染状况为无明确意义的非典型细胞的改变(ASCUS)44.7%,不典型鳞状细胞不除外高度病变(ASCUS-H)7.8%,低度鳞状上皮内病变(LSIL)19.7%,高

  6. Prolactin and prolactin receptor expression in cervical intraepithelial neoplasia and cancer.

    Science.gov (United States)

    Ascencio-Cedillo, Rafael; López-Pulido, Edgar Ivan; Muñoz-Valle, José Francisco; Villegas-Sepúlveda, Nicolás; Del Toro-Arreola, Susana; Estrada-Chávez, Ciro; Daneri-Navarro, Adrian; Franco-Topete, Ramón; Pérez-Montiel, Delia; García-Carrancá, Alejandro; Pereira-Suárez, Ana Laura

    2015-04-01

    Prolactin receptor (PRLR) overexpression could play a role in tumorigenesis. The aim of this study was to determine prolactin (PRL) and PRLR expression in biopsies from patients with precursor lesions and uterine cervical cancer. PRLR expression was analyzed in 63 paraffin-embedded biopsies of uterine cervical tissue. In total, eleven low-grade squamous intraepithelial lesions (LSIL), 23 high-grade squamous intraepithelial lesions (HSIL), 21 uterine cervical cancers (UCC) and 8 normal epithelium (NE) were examined using immunoperoxidase staining and Western blot analysis. Additionally, PRL expression was identified in human cervical cancer serum and tissues. The PRLR expression was found to be significantly increased in cervical cancer in comparison with normal tissue and precursor lesions (P prolactin expression was similar in precursor lesions and cervical cancer by Western blot analysis. Our data suggest a possible role for PRLR in the progression of cervical cancer.

  7. The function of HR-HPV and LCT test and histology biopsy with colposcopy of cervical in diagnosis of cervical lesion%HR-HPV检测联合宫颈液基细胞学及经阴道镜活检诊断宫颈病变

    Institute of Scientific and Technical Information of China (English)

    陈勇霞; 刘颖; 梁爽爽

    2012-01-01

    Objective To evaluate the function and application of high risk human papillomavirus (HR-HPV) test, liquid-based cytology test (LCT) and histological biopsy with colposcopy. Methods 601 patients with abnormal LCT results in the outpatient department of gynecology from January 2009 to October 2011 were reexamined using HR-HPV test. The results of HR-HPV and LCT tests were compared with histological biopsy under colposcope. Results 67.2% (404/601) of the patients were found to be HR-HPV positive. The infection rate of patients with ASC-US, LSIL, ASC-H, HSIL and CA was 45.5%, 66.2%, 74.4%, 89.1% and 100.0%, respectively, and the difference was significant (P<0.05). The sensitivity of diagnosing CIN and cervical lesions in the combined LCT and HR-HPV test was 86.1%. The result was higher than the LCT test (77.1%) and HR-HPV test (72.0%) (P<0.05). The false negative rate (13.9%) was lower than the LCT and HR-HPV test (P<0.05). The compliance rate was 50.8% (102/201) between cervical inflammation, 22 cases (11.0%) had CIN I , 40 cases (19.9%) had CIN II , 34 cases (16.9%) had CIN I , and 2 cases (1.0%) had cervical cancer. In the HR-HPV test, the detection rate was 59.2% (119/201); with a sensitivity of 71.7% (71/99), specficity of 53.0% (54/102) and an accuracy of 64.7%. Conclusion The pathogenic development of cervical lesion is correlated closely with HR-HPV infection. LCT and HR-HPV tests, together with histological examination can be used to improve the diagnosis cervical lesion.%目的 评估高危型人乳头状瘤病毒(HR-HPV)检测、液基细胞学(LCT)及阴道镜下组织活检联合应用在宫颈病变诊断中的作用及意义.方法 对2009年1月至2011年10月在广州医学院第二附

  8. Are all left supraclavicular lymphadenopathies Virchow's? A retrospective study of 320 left cervical lymphadenopathy

    OpenAIRE

    Veena Raja; Chinnaiya Subramaniam; Babu Rajendra Prasad; Mari Lingiah Harendra Kumar; Balluru Vasudeva Swaroop Raj

    2016-01-01

    Background: Cervical Lymphadenopathy is one of the commonest and sometimes only clinical finding for a benign and malignant lesion. Fine needle aspiration cytology (FNAC) is a simple, safe, rapid and inexpensive method for establishing the diagnosis of lesions. Metastasis is more common in cervical lymph node and enlarged cervical nodes in an elderly patient must be considered as metastatic until proved otherwise. Methods: Retrospectively, 320 cases of left cervical lymph node FNAC were colle...

  9. An investigation into magnetic electrolytic abrasive turning

    International Nuclear Information System (INIS)

    The magnetic electrolytic abrasive turning (MEAT) process as a non-traditional machining is used to obtain surface finishing like mirror. MEAT provides one of the best alternatives for producing complex shapes with good finish in advanced materials used in aircraft and aerospace industries. The improvement of machining accuracy of MEAT continues to be a major challenge for modern industry. MEAT is a hybrid machining which combines two or more processes to remove material. The present research focuses on the development of precision electrochemical turning (ECT) under the effects of magnetic field and abrasives. The effect of magnetic flux density, electrochemical conditions and abrasive parameters on finishing efficiency and surface roughness are investigated. An empirical relationship is deduced

  10. An investigation into magnetic electrolytic abrasive turning

    Science.gov (United States)

    Mahdy, M. A. M.; Ismaeial, A. L.; Aly, F. F.

    2013-07-01

    The magnetic electrolytic abrasive turning (MEAT) process as a non-traditional machining is used to obtain surface finishing like mirror. MEAT provides one of the best alternatives for producing complex shapes with good finish in advanced materials used in aircraft and aerospace industries. The improvement of machining accuracy of MEAT continues to be a major challenge for modern industry. MEAT is a hybrid machining which combines two or more processes to remove material. The present research focuses on the development of precision electrochemical turning (ECT) under the effects of magnetic field and abrasives. The effect of magnetic flux density, electrochemical conditions and abrasive parameters on finishing efficiency and surface roughness are investigated. An empirical relationship is deduced.

  11. Basics of cutting and abrasive processes

    CERN Document Server

    Toenshoff, Hans Kurt

    2013-01-01

    Manufacturing is the basic industrial activity generating real value. Cutting and abrasive technologies are the backbone of precision production in machine, automotive and aircraft building as well as of production of consumer goods. We present the knowledge of modern manufacturing in these technologies on the basis of scientific research. The theory of cutting and abrasive processes and the knowledge about their application in industrial practice are a prerequisite for the studies of manufacturing science and an important part of the curriculum of the master study in German mechanical engineering. The basis of this book is our lecture “Basics of cutting and abrasive processes” (4 semester hours/3 credit hours) at the Leibniz University Hannover, which we offer to the diploma and master students specializing in manufacturing science.

  12. HPV infection subtype distribution and its relation with cervical lesions%HPV 感染亚型分布及其与宫颈病变关系的研究

    Institute of Scientific and Technical Information of China (English)

    段山红; 安瑞芳

    2015-01-01

    among gynecological outpatients in Xianyang district and its relation with cervical lesions.Methods Between January 2009 and February 2013, totally 3 573 outpatients undergoing HPV genotyping and liquid-based cytology check were selected in three tertiary hospitals in Xianyang district.There were 966 cases with HPV genotype positive ( including 19 kinds of high-and low-risk subtype) and 572 cases with cytological positive ( ASCUS) .Both positive results were found in 354 cases.For 1 169 patients with any positive results, colposcopy and cervical biopsy were performed.The correlation between subtypes of HPV infection and cervical lesions was analyzed according to pathological examination results.Results HPV testing positive rate was 27.04%.HR-HPV positive rate was 24.55%, and HPV16 positive rate was 12.26% accounting for 45.34% of HR-HPV.The highest positive rate of HPV was found among women aged below 30.With age increasing, HPV positive rate declined gradually.There were significant differences in HPV positive rate among different age groups except for group aged≤20 years and group aged 21-30 years (χ2 value was 5.08, 8.93, 37.48 and 4.28, respectively, all P<0.05).The detection rates of HPV16 in CINⅡ, CINⅢand squamous cell cancer (SCC) were highest, and they were 50.59%, 78.13%and 81.82%, respectively.Compared with HPV58, HPV18, HPV52 and HPV33, HPV16 caused CINⅡor above more easily, and the difference was statistically significant (χ2 value was 53.93,62.84,93.01 and 93.01, respectively, all P<0.05).Conclusion The most common high-risk HPV genotypes infected in outpatients in Xianyang district are HPV16, HPV52, HPV58, HPV18 and HPV33.HPV16 plays a key role in the genesis and development of cervical cancer and precancerous lesions.HPV58, HPV18, HPV52, and HPV33 are also pathogenic in this area.High-risk HPV genotyping is therefore very important in cervical cancer screening and the process of prevention and diagnosis.

  13. Evaluation of computer-assisted ThinPrep imaging system in the screening of cervical cancer and precancerous lesion%TIS在宫颈癌和癌前病变筛查中的价值评价

    Institute of Scientific and Technical Information of China (English)

    米贤军; 王莹; 沈铿; 吴秋良; 肖琳; 陈昂; 徐秀梅; 孪峰; 钟守军

    2013-01-01

    目的 评价新柏氏电脑辅助阅片系统(TIS)对宫颈癌及癌前病变的诊断价值.方法 收集2011年6月至2012年6月参加中山市大规模免费宫颈癌筛查中妇女的资料,选择做过TIS检测并追踪到有确切组织病理学诊断结果的600例病例为研究对象,将ASCUS/AGC+病变列为细胞学阳性病例,将CIN1+病变列为病理学阳性病例,以病理学检查为金标准,评价TIS检测的诊断价值.结果 600例妇女中,TIS检测结果为癌(CA)9例,高度鳞状上皮内病变(HSIL) 66例,低度鳞状上皮内病变(LSIL) 104例,不除外高度鳞状上皮内病变的不典型鳞状细胞(ASC-H) 21例,非典型鳞状细胞/非典型腺细胞(ASCUS/AGC) 30例,正常(WNL) 370例.以病理学诊断结果为金标准,TIS检测的灵敏度为93.95%,特异度为92.73%,约登指数为0.867,阳性似然比12.92,阴性似然比0.065,Kappa值0.854,总符合率为93.17%,阳性预测值87.83%,阴性预测值96.49%.TIS与病理学结果的符合率在CA、HSIL、LSIL中分别为100%、90.91%和72.12%,TIS对CA和HSIL的符合率高于LSIL (P<0.01).结论 TIS对宫颈癌及癌前病变有较高的诊断价值,特别是对CA和HSIL.它是一种高效、高质量和高准确率的宫颈癌及癌前病变筛查技术.%Objective To study the diagnostic value of computer-assisted ThinPrep imaging system(TIS) in the screening of cervical cancer and precancerous lesion.Methods The clinical data of 600 cases who participated voluntarily in the screening of large-scale cervical cancer for free in Zhongshan were collected from June 2011 to June 2012.ASCUS/AGC and above were postive cytology and the postive results of pathological examination were CIN and above.Histopathological results were used as gold standard to calculate the diagnostic value of TIS technique.Results A total of 600 cases were detected by TIS,and the testing results showed 9 cases of CA,66 cases of HSIL,104 cases of LSIL,21 cases of ASC-H,30 cases of ASCUS

  14. Universal scaling relations for pebble abrasion

    Science.gov (United States)

    Litwin, K. L.; Jerolmack, D. J.

    2012-12-01

    The process of abrasion of gravel in bed load transport results from particle-to-particle collisions, where the energy involved is sufficient to cause chipping and spallation but not fragmentation of parent grains. The removed rock material is not infinitesimal; daughter products as large as coarse sand can be produced. Although previous work has shown that lithology, grain shape, and energy of collision are contributing factors that control abrasion rates of river-bed material, little is known regarding the relationship between these factors and diminution rates. Here we explicitly isolate and investigate how these three factors influence rates of abrasion and the size distribution of daughter products, with laboratory experiments. The apparatus is a double pendulum (Newton's cradle) that produces well-controlled binary collisions. A high-speed camera precisely measures collision energy, while mass of parent rocks. and the size and shape distributions of daughter products, are measured periodically. We examined abrasion of initially square-cut 'rocks' as they underwent successive collisions in the binary collision apparatus. We have examined mass loss rate for varied lithologies, and observe a similar power-law relationship between impact energy and mass abraded. When normalized by sensible material properties, mass loss curves for all materials collapse onto a single curve, suggesting that the underlying mechanics of abrasion for different materials are the same. The relationship does not display the linear trend expected from pure energetics, and we suggest that this is a shape effect as protruding - and hence easily eroded - corners are worn away. Analysis of daughter-product particle size distributions for different lithology fragments - including natural rocks and also bricks - show the same functional form. Surprisingly, it is the power-law relation expected for brittle materials undergoing fragmentation. This suggests that brittle fracture theory also

  15. Abrasion test of flexible protective materials on hydraulic structures

    Institute of Scientific and Technical Information of China (English)

    Xin WANG; Shao-ze LUO; Guang-sheng LIU; Lu-chen ZHANG; Yong WANG

    2014-01-01

    In this study, several kinds of flexible protective materials sprayed with polyurea elastomers (hereinafter referred to as polyurea elastomer protective material) were adopted to meet the abrasion resistance requirement of hydraulic structures, and their abrasion resistances against the water flow with suspended load or bed load were studied systematically through tests. Natural basalt stones were adopted as the abrasive for simulation of the abrasion effect of the water flow with bed load, and test results indicate that the basalt stone is suitable for use in the abrasion resistance test of the flexible protective material. The wear process of the polyurea elastomer protective material is stable, and the wear loss is linear with the time of abrasion. If the wear thickness is regarded as the abrasion resistance evaluation factor, the abrasion resistance of the 351 pure polyurea is about twice those of pure polyurea with a high level of hardness and aliphatic polyurea, and over five times that of high-performance abrasion-resistant concrete under the abrasion of the water flow with suspended load. It is also about 50 times that of high-performance abrasion-resistant concrete under the abrasion of the water flow with bed load. Overall, the abrasion resistance of pure polyurea presented a decreasing trend with increasing hardness. Pure polyurea with a Shore hardness of D30 has the best abrasion resistance, which is 60 to 70 times that of high-performance abrasion-resistant concrete under the abrasion of the water flow with bed load, and has been recommended, among the five kinds of pure polyurea materials with different hardness, in anti-abrasion protection of hydraulic structures.

  16. Abrasion test of flexible protective materials on hydraulic structures

    Directory of Open Access Journals (Sweden)

    Xin WANG

    2014-01-01

    Full Text Available In this study, several kinds of flexible protective materials sprayed with polyurea elastomers (hereinafter referred to as polyurea elastomer protective material were adopted to meet the abrasion resistance requirement of hydraulic structures, and their abrasion resistances against the water flow with suspended load or bed load were studied systematically through tests. Natural basalt stones were adopted as the abrasive for simulation of the abrasion effect of the water flow with bed load, and test results indicate that the basalt stone is suitable for use in the abrasion resistance test of the flexible protective material. The wear process of the polyurea elastomer protective material is stable, and the wear loss is linear with the time of abrasion. If the wear thickness is regarded as the abrasion resistance evaluation factor, the abrasion resistance of the 351 pure polyurea is about twice those of pure polyurea with a high level of hardness and aliphatic polyurea, and over five times that of high-performance abrasion-resistant concrete under the abrasion of the water flow with suspended load. It is also about 50 times that of high-performance abrasion-resistant concrete under the abrasion of the water flow with bed load. Overall, the abrasion resistance of pure polyurea presented a decreasing trend with increasing hardness. Pure polyurea with a Shore hardness of D30 has the best abrasion resistance, which is 60 to 70 times that of high-performance abrasion-resistant concrete under the abrasion of the water flow with bed load, and has been recommended, among the five kinds of pure polyurea materials with different hardness, in anti-abrasion protection of hydraulic structures.

  17. Cervical spondylolysis in child with four levels of simultaneous involvement: a case report

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Gang Deuk; Kim, Hye Won; Jang, Sung Jo; Oh, Jung Taek [Wonkwang University School of Medicine, Gunsan (Korea, Republic of)

    2006-12-15

    Cervical spondylolysis is a rare condition, and less than 100 cases have been reported in the world literature. Cervical spondylolysis is defined as a well corticated defect in the posterior element of a cervical vertebra. Although the etiology of cervical spondylolysis is unknown, its association with dysplastic changes and spina bifida occulta suggest that the lesion is congenital. Here, we describe the radiographs and CT images of cervical spondylolysis involving four levels in a 9 year old boy.

  18. Cervical spondylolysis in child with four levels of simultaneous involvement: a case report

    International Nuclear Information System (INIS)

    Cervical spondylolysis is a rare condition, and less than 100 cases have been reported in the world literature. Cervical spondylolysis is defined as a well corticated defect in the posterior element of a cervical vertebra. Although the etiology of cervical spondylolysis is unknown, its association with dysplastic changes and spina bifida occulta suggest that the lesion is congenital. Here, we describe the radiographs and CT images of cervical spondylolysis involving four levels in a 9 year old boy

  19. 液基细胞学联合人乳头瘤病毒检查在妊娠期宫颈病变筛查中的作用%Effect of liquid-based cytology combined with human papillomavirus test in screening of cervical lesions during pregnancy

    Institute of Scientific and Technical Information of China (English)

    关嵩青; 叶菲

    2012-01-01

    目的:探讨液基细胞学(TCT)联合人乳头瘤病毒(HPV)检查在妊娠期宫颈病变筛查中的价值.方法:采用TCT联合HPV检查的方法,对在深圳市第二人民医院行产前检查的500例妊娠期妇女进行宫颈病变筛查,以宫颈组织病理学检查结果作为确诊的金标准.结果:500例中,高危HPV阳性检出率为28.00%,TCT阳性检出率为8.40%,HPV与TCT同时阳性与阴道镜活检病理结果的阳性符合率为89.29%.结论:产前检查是进行宫颈病变筛查的良好时机,TCT联合HPV检查与阴道镜活检病理结果的阳性符合率高,用于妊娠期宫颈病变筛查是安全和有效的.%Objective: To explore the value of liquid — based cytology combined with human papillomavirus (HPV) test in screening of cervical lesions during pregnancy. Methods: Liquid-based cytology combined with HPV test was used to carry out screening of cervical lesions in 500 pregnant women in the hospital, the pathological result of cervical tissue was designed as gold standard of definite diagnosis. Results: Among 500 pregnant women, the detection rate of high risk HPV was 28. 00% , and the positive detection rate of liquid-based cytology was 8. 40% , the positive coincidence rate between positive HPV test, liquid-based cytology and pathological result after colposcopic biopsy was 89. 29%. Conclusion: Prenatal examination is a good opportunity for screening of cervical lesions; the positive coincidence rate between liquid-based cytology combined with HPV test and pathological result after colposcopic biopsy was high; the method is safe and effective for screening of cervical lesions during pregnancy.

  20. Research Progress in Correlation of Vaginal Lactobacillus with HPV Infection, Cervical Cancer and Precancerous Lesions%阴道乳酸杆菌与HPV感染、宫颈癌及癌前病变的相关性研究进展

    Institute of Scientific and Technical Information of China (English)

    王颖; 刘植华

    2013-01-01

    乳酸杆菌及其代谢产物具有抑制肿瘤的作用,通过产生各种代谢产物干扰肿瘤代谢,以及激活机体免疫功能而发挥作用.乳酸杆菌在防治各种肿瘤中的作用也已引起重视.阴道乳酸杆菌是构成阴道微环境的重要因素,其数量的减少与HPV感染、宫颈癌前病变及宫颈癌有关.文章就阴道乳酸杆菌与HPV感染、宫颈癌及癌前病变的关系进行综述.%Previous studies had demonstrated that lactobacillus had antitumor effect.It interferences tumor metabolism through the production of metabolites and activates the immune function.Effect of lactobacillus in the prevention and treatment for various tumors has aroused attention.Lactobacillus is a dominated factor in normal women's vaginal environment.Decreasing of vaginal lactobacillus related to human papilloma virus (HPV) infection,cervical cancer and precancerous lesions.The relationship of lactobacillus with HPV infection,cervical cancer and cervical precancerous lesions is reviewed.

  1. Recent progress of abrasion-resistant materials: learning from nature.

    Science.gov (United States)

    Meng, Jingxin; Zhang, Pengchao; Wang, Shutao

    2016-01-21

    Abrasion-resistant materials have attracted great attention for their broad applications in industry, biomedicine and military. However, the development of abrasion-resistant materials that have with unique features such as being lightweight and flexible remains a great challenge in order to satisfy unmet demands. The outstanding performance of natural abrasion-resistant materials motivates the development of new bio-inspired abrasion-resistant materials. This review summarizes the recent progress in the investigation of natural abrasion-resistant materials to explore their general design principles (i.e., the correlation between chemical components and structural features). Following natural design principles, several artificial abrasion-resistant materials have shown unique abrasion-resistant properties. The potential challenges in the future and possible solutions for designing bio-inspired abrasion-resistant materials are also briefly discussed. PMID:26335377

  2. Cervical Stenosis

    Science.gov (United States)

    ... Accumulation of pus in the uterus is called pyometra. Symptoms Before menopause, cervical stenosis may cause menstrual ... present but not cause symptoms. A hematometra or pyometra can cause pain or cause the uterus to ...

  3. Cervical spondylosis

    Science.gov (United States)

    ... Past neck injury (often several years before) Past spine surgery Ruptured or slipped disk Severe arthritis Small fractures ... Kshettry VR. Cervical spondylosis. In: Benzel EC, ed. Spine Surgery . 3rd ed. Philadelphia, PA: Elsevier Saunders; 2012:chap ...

  4. Cervical Cancer

    Science.gov (United States)

    ... Cervical cancer is caused by a virus called HPV. The virus spreads through sexual contact. Most women's bodies are able to fight HPV infection. But sometimes the virus leads to cancer. You're at higher risk ...

  5. Cervical Cancer

    Science.gov (United States)

    ... 162 KB) This information in Spanish (en español) Female reproductive system Select image to view larger Related ... D., FACS, Captain, U.S. Public Health Service Medical Director, National Breast and Cervical Cancer Early Detection Program, ...

  6. Abrasion of 6 dentifrices measured by vertical scanning interference microscopy

    OpenAIRE

    Florence Pascaretti-Grizon; Guillaume Mabilleau; Daniel Chappard

    2013-01-01

    OBJECTIVES: The abrasion of dentifrices is well recognized to eliminate the dental plaque. The aims of this study were to characterize the abrasive powders of 6 dentifrices (3 toothpastes and 3 toothpowders) and to measure the abrasion on a test surface by Vertical Scanning Interference microscopy (VSI). MATERIAL AND METHODS: Bright field and polarization microscopy were used to identify the abrasive particles on the crude dentifrices and after prolonged washes. Scanning electron microscop...

  7. Concrete ice abrasion rig and wear measurements

    NARCIS (Netherlands)

    Shamsutdinova, G.; Rike, P.B.; Hendriks, M.A.N.; Jacobsen, S.

    2015-01-01

    The wear of concrete material due to ice movement is a challenge for offshore and coastal structures. Concrete surfaces exposed to moving ice are subjected to wear at various rates depending on concrete and ice properties. At NTNU, Department of Structural Engineering, concrete ice abrasion phenomen

  8. Performance Enhancement of Abrasive Waterjet Cutting

    NARCIS (Netherlands)

    Vu Ngoc Pi

    2008-01-01

    Abrasive Waterjet (AWJ) Machining is a recent non-traditional machining process. This technology is widely used in industry for cutting difficult-to-machine-materials, milling slots, polishing hard materials etc. AWJ machining has many advantages, e.g. it can cut net-shape parts, no heat is generate

  9. Clinical Significance of HC2-HPV Assay Combined with Liquid Based Cytology in the Screen of Cervical Cancer and Precancerous Lesions%HC2-HPV 检测法联合液基细胞学在宫颈癌及癌前病变筛查中的临床意义

    Institute of Scientific and Technical Information of China (English)

    徐艺; 杨静秀; 王玉平

    2016-01-01

    目的:探讨HC2-HPV检测法联合液基细胞学在筛查宫颈癌及癌前病变中的意义。方法应用HC2-HPV检测法联合液基细胞学技术对体检的患者进行免费宫颈癌及癌前病变的筛查。对不典型增生患者或(和) HC2-HPV检测阳性的患者行阴道镜下宫颈活检。结果液基细胞学检查对宫颈高度病变的检出率为11.0%(11/100);HC2-HPV检测对宫颈高度病变的检出率为27.2%(22/81),对宫颈癌的检出率高达100.0%。 HC2-HPV检测法联合液基细胞学检测阳性与病理检查阳性符合率为64.2%(52/81),显著优于TCT(52.0%)单独检查时的阳性符合率(P<0.05,χ2=11.39)。结论 HC2-HPV检测法联合液基细胞学检测对宫颈癌及癌前病变的筛查具有重要的临床推广价值。%Objective To investigate the significance of HC 2-HPV assay combined with liquid based cytology in the screen of cervical cancer and precancerous lesions .Methods HC2-HPV assay combined with liquid based cytology was used in the screen of cervical cancer and precancerous lesions .Cervical biopsy was performed in patients with atypical hyperplasia or (and) HC2-HPV positive patients.Results Detection rate of liquid based cytology for cervical lesion was 11.0%(11/100),and detection rates of HC2-HPV for cervical lesion and cervical cancer were 27.2%(22/81) and 100.0%.The coincidence rate of HC2-HPV combined with liquid based cytology and biopsy results was 64.2%(52/81),which was significantly higher than that of TCT(52.0%)(P<0.05,χ2 =11.39).Conclusion HC2-HPV assay combined with liquid based cytology test has important clinical value in the screen of cervical cancer and precancerous lesions .

  10. 叶酸与DNA甲基转移酶1在宫颈癌及癌前病变中的作用%Effect of folic acid and DNA methyltransferase 1 on cervical cancer and its precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    王金桃; 霍晓旭; 丁玲; 郝敏; 樊金荣; 张丽; 赵维敏

    2011-01-01

    Objective To explore the effect of folic acid and DNA methyltransferase 1 (DNMT1) on cervical cancer and cervix precancerous lesion. Methods 100 patients with cervix squamouscell carcinoma (SCC), 101 patients with cervical intraepithelial neoplasm (CIN) and 109 patients with cervix inflammation (CI) diagnosed by histology were included in this study. Radioimmunoassay (RIA), polymerase chain reaction (PCR) and Western blot were used to detect the levels of serum folate, HPV16 infection and the expression of DNMT1 protein,respectively. Results The average levels of serum folate were (2.60 ± 1.61) ng/ml, (3.14 + 2.08) ng/ml and (3.32+1.74) ng/ml,and the expression of DNMT1 protein were 2.40 + 0.99,1.88 + 0.33 and 0.89 ± 0.29 in the group of SCC, CIN and CI, respectively.The relationship of folate levels and DNMT1 protein expression showed inverse correlation (r=-0.186, P=0.00l). The results in our study indicated that there was an additive interaction between low-level of serum folate and high-expressionof DNMT1 protein related to the risk of CIN and SCC, with OR value as 2.50(95%C/: 1.21-9.22) and 6.03 (95%C/: 2.79-21.72) respectively. The relative excessrisk of interaction (RERI) , attributableproportion of interaction (API) and synergy index (S) were 0.92, 0.36 and 2.59 in the CIN group while 2.47, 0.41 and 1.96 in the SCC group. Conclusion The low level of serum folate and high expression of DNMT1 protein seemed to be associated with high risk of cervical cancer and its precancerous lesion. It suggested that there might be a synergistic action between serum folate and DNMT1 in the progression of cervix carcinogenesis.%目的 探讨叶酸和DNA甲基转移酶1(DNMT1)与宫颈癌发生发展的关系及其在宫颈癌变中的相互作用.方法 选择经病理学确诊的宫颈鳞状细胞癌(SCC)患者100例、宫颈上皮内瘤样变(CrN)患者101例和宫颈炎(CI)患者109例为研究对象.在收集全部对象人口学特征、生殖因素等相关资料

  11. Telomerase activity as a biomarker for (pre)neoplastic cervical disease in scrapings and frozen sections from patients with abnormal cervical smear

    NARCIS (Netherlands)

    Wisman, GBA; Hollema, H; de Jong, S; ter Schegget, J; Tjong-A-Hung, SP; Ruiters, MHJ; Krans, M; de Vries, EGE; van der Zee, AGJ

    1998-01-01

    Purpose: To evaluate the diagnostic value of semiquantitative telomerase activity assessment in cervical scrapings together with human papillomavirus (HPV) typing for detection of (pre)neoplastic cervical lesions and to compare telomerase activity in cervical scrapings and frozen specimens from the

  12. 液基细胞学联合初善仪检测在宫颈癌筛查中的应用%Value of TruScreen combined with Thinprep cytology test in screening of cervical lesions

    Institute of Scientific and Technical Information of China (English)

    杜欣欣; 赛晓勇; 刘爱军; 张云; 付晓宇; 李江华; 孟元光

    2015-01-01

    目的:探讨液基细胞学(TCT)与初善仪(TS)在宫颈病变筛查中的联合应用价值。方法选择2013年12月~2014年11月就诊于解放军总医院妇科门诊阴道镜室的患者218例,所有患者均给予TCT及TS检查,并根据TCT及TS检查结果进行分组,结合病理学结果分析两种检测方法细胞学诊断价值的差异。结果TCT结果显示,218例患者中,不能明确意义的非典型鳞状细胞(ASC-US)76例,非典型鳞状细胞不除上皮内高度病变(ASC-H)43例,低度鳞状上皮内病变(LSIL)58例,高度鳞状上皮内病变(HSIL)41例;TS检测异常者94例。病理结果正常者106例,LSIL/CIN 141例,HSIL/CIN 2~371例。病理结果正常患者中,细胞学ASC-H与LSIL的检出率分别为53.5%和56.9%;低级别病变患者中,ASC-US和LSIL的检出率显著高于ASC-H及HSIL组(P=0.005);高级别病变患者中,ASC-H及HSIL的检出率显著高于ASC-US和LSIL(P=0.004)。经TS分层后,TS阴性而病理结果正常的患者中,TCT各组的检出率均提高;TS阳性而病理低级别病变患者中,ASC-US与LSIL的检出率升高,ASC-H与HSIL呈下降趋势,高级别病变患者中,TBS分级四组的检出率均升高。结论联合应用TS可以减少阴道镜门诊的过度转诊并能够更灵敏地发现高级别病变患者。%Objective To investigate the value of TruScreen (TS) combined with Thinprep cytology test in screening of cervical lesions. Methods 218 patients from December 2013 to November 2014 in Outpatient Vaginoscopy Room of PLA General Hospital, all patients were given the detection of TCT and TS, they were divided into different group ac-cording the detection results of TCT and TS, and diagnostic value of TCT and TS were evaluated by pathology result as a golden standard. Results The results of TCT showed that, there were 76 cases of ASC-US, 43 cases of ASC-H, 58 cases of LSIL and 41 cases of HSIL in total of 218 patients; TS showed that 94 cases were abnormal. Pathological re

  13. 不同人乳头状瘤病毒检测方法对宫颈高度病变的诊断价值%Assays for detection of human papillomavirus infection in diagnosis of high grade cervical lesions

    Institute of Scientific and Technical Information of China (English)

    徐华林; 卢莺燕; 林红梅; 陶萍萍

    2011-01-01

    目的 评价杂交捕获Ⅱ代(HC-Ⅱ)与聚合酶链反应(PCR)技术对人乳头状瘤病毒(human papillomavirus,HPV)DNA检测及宫颈高度病变的诊断价值.方法 选取宫颈薄层液基细胞学检查(TCT)异常的200例妇女为研究对象,同时用HC-Ⅱ、导流杂交基因芯片技术(HybriMax)、实时荧光定量PCR法(FQ-PCR)和流式荧光杂交法检测宫颈细胞HPV DNA.以病理组织学检测结果为金标准,比较4种检测方法对宫颈上皮内瘤变(CIN)≥Ⅱ的诊断效果.采用SPSS 13.0软件进行统计学处理.结果 HybriMax、FQ-PCR、流式荧光法和HC-Ⅱ对200例HPV DNA阳性检出率分别为72.5%(145/200)、71.5%(143/200)、70.0%(140/200)和69.0%(138/200).4种HPV DNA检测方法对不同程度宫颈病变的HPV检测阳性率差异无统计学意义(x2=0.252、0134、0.012和0.027,P值均>0.05),但在诊断CINⅡ及以上病变的敏感度、约登指数和阴性预测值等方面比较差异有统计学意义(x2=7.923、7.819和8.108,P<0.05).结论 对组织病理学诊断结果为CINⅡ及以上的宫颈病变,HC-Ⅱ的诊断价值优于PCR方法.%Objective To evaluate the application of different assays for detection of human papillomavirus(HPV)in diagnosis of high grade cervical lesions.Methods Two hundred subjects with abnormal thinprep liquid-based cytology test(TCT)Results were selected for HPV DNA detection by hybrid capture 2(HC-Ⅱ) and Methods based on PCR including flow-through hybridization and gene chip (HybriMax),real-time fluorescent quantitative PCR(FQ-PCR)and flow fluorescent hybridization assay.Cytopathological Results were used as gold standards to evaluate the test performance of the above assays for diagnosing cervical intraepithelial neoplasia(CIN)≥Ⅱ. SPSS 13.0 software was used for statistical analysis.Results HPV DNA positive rates of 200 samples by HybriMax,FQ-PCR,flow fluorescent hybridization assay and HC-Ⅱ were 72.5%(145/200),71.5%(143/200),70.0%(140/200)and 69

  14. Thermal aspects of ice abrasive water jet technology

    Directory of Open Access Journals (Sweden)

    Marko Jerman

    2015-08-01

    Full Text Available During the last few years, different research groups have been developing systems for the transition of abrasive water jet into ice abrasive water jet. The aim of this new technology is to make the technology cleaner from both practical and ecological points of view. Mineral abrasive is replaced with ice grains that melt away after the machining process, leaving the workpiece uncontaminated. Several different approaches to this technology were studied. Thermal aspects of integrating the ice abrasive water jet technology into commercially available machines were considered. The results and analyses of water temperature measurements on the ice abrasive water jet machine are presented in this article.

  15. Usage of abrasion-resistant materials in agriculture

    Directory of Open Access Journals (Sweden)

    J Votava

    2014-06-01

    Full Text Available Agricultural soil-processing machines are subject to an extensive abrasive wear. This paper analyses technical materials and their fitness to exchangeable parts of plough bottoms, such as edge-tools and whole plough cutting edges. There were tested abrasion-resistant steels with different microstructures: austenite, martensite-bainite, and carbide. Steel with the pearlite-ferrite structure was used as an etalon. Abrasion resistance tests were processed in compliance with the norm CSN 01 5084, which is a test of abrasion wear on abrasive cloth.

  16. Gingival abrasion and plaque removal with manual versus electric toothbrushing.

    Science.gov (United States)

    Niemi, M L; Ainamo, J; Etemadzadeh, H

    1986-08-01

    A clinical trial was designed to test the relative numbers of gingival lesions caused during standardized brushing of the teeth of 22 volunteer dental nurse students with a manual soft multitufted, a manual soft V-shaped, and an electric toothbrush. First, the left or the right side of the jaws of each subject was brushed by a dental hygienist using the manual V-shaped or the electric brush, and the other side using the manual multitufted brush. At the 2nd brushing 1 week later, the same hygienist used the multitufted brush for brushing the side contralateral to the one in which it was used the 1st week and the V-shaped manual brush instead of the electric and vice versa. After each brushing, the number of new gingival lesions was recorded and the cleansing effect evaluated by assessment of the amount of remaining plaque. This examiner was unaware of the type of brush used. The V-shaped manual toothbrush was found to have caused more gingival abrasion than the electric toothbrush (P less than 0.005) and a similar difference was found between the multitufted manual and the electric toothbrush (P less than 0.05). There was no clinically significant difference between the plaque removing effects of the 3 brushes tested. PMID:3463575

  17. Properties, structure and machnining capabilities sintered corundum abrasives

    Directory of Open Access Journals (Sweden)

    Cz.J. Niżankowski

    2010-07-01

    Full Text Available The diversity of sintered corundum abrasives used in both bonded and in the embankment of abrasive tools currently poses substantialproblems for their choice of technology to specific tasks. Therefore performed a comparative study of ownership structures and capacitiesof elected representatives machnining sintered corundum abrasives of different generations, and this is normal sintered alumina,submicrocrystalline alumina sintered and nanocrystalline alumina sintered. Were studied some properties of a set of abrasive particles,physicochemical properties and structural and mechanical and technological properties. The studies used the method of microscopicmeasurement to determine the shape of abrasive particles, the pycnometer to determine the density of abrasive, a spectrometer todetermine the chemical composition of the magnetic analyzer for determining the magnetic fraction, scanning electron microscope toanalysis of abrasive grains and a special position to designate the machining capacity abrasive grains. The results showed a significantincrease in machining capacity sintered corundum abrasives with increasing degree of fragmentation of the crystallites sintered corundum abrasives and distinctive bands in the emerging microchip. The originality of the development provides a comparative summary ofproperties of sintered corundum abrasives of different generations and functions obtained by the author making the change in value indexof machininhcapacity grit from cutting speeds for different generations of sintered corundum.

  18. PHAEOHYPHOMYCOSIS: CUTANEOUS, SUBCUTANEOUS, NASOPHARYNGEAL LESIONS

    Directory of Open Access Journals (Sweden)

    M. Rasoolinejad

    1999-06-01

    Full Text Available Phaeohyphomycosis is an amalgam of clinical diseases caused by a wide variety of dematiaceous fungi. We are reporting on a 16 year-old patient from Amol with subcutaneous cervical nodes and nasopharyngeal lesions of phaeohypho"nmycosis that were confirmed by pathological examination, direct smear, and culture. After treatment with an oral triazole (Itraconazole for 4 months, all nodes and lesions disappeared and treatment was stopped A new lesion appeared on his chest wall 8 months, therapy with itraconazole was restarted and commuted for a long time.

  19. INTERNAL FINISHING OF CYLINDRICAL PIPES USING SINTERED MAGNETIC ABRASIVES

    Directory of Open Access Journals (Sweden)

    PALWINDER SINGH

    2011-07-01

    Full Text Available An internal magnetic abrasive finishing (MAF process was proposed to produce highly finished inner surfaces of workpieces used in critical applications. The process principle and the finishing characteristics of magnetic abrasive finishing of cylindrical pipes using sintered magnetic abrasives are described in this research work. Thesintered magnetic abrasive is a mixture of Al2O3 abrasive and ferromagnetic particles. The Al2O3 based sintered magnetic abrasives have been developed in sintering machine. The surface roughness measurements resulting from finishing experiments demonstrate the effects of the abrasive behavior on the surface modifications. Thesurface finish was analysed in terms of percent improvement in surface finish (PISF. Also surface finish was analysed using Response Surface Methodology (RSM. The obtained maximum PISF was 95% and minimum surface roughness was 0.05 μm Ra. To further study the improvement in surface finish, the surface was microscopically examined using X-Ray Diffraction (XRD.

  20. The effect of bleaching on toothbrush abrasion of resin composites

    Directory of Open Access Journals (Sweden)

    Hila Hajizadeh

    2013-01-01

    Full Text Available Aim: This experimental study was designed to focus on the effects of bleaching on toothbrush abrasion in three types of composites with different filler size. Materials and Methods: Forty eight disks were prepared from three types of composite and divided into 6 groups. In the first three groups the abrasion test was done. The remaining groups were bleached and the abrasion test was performed. The weight of the samples before and after abrasion was measured. Statistical analysis was done with one-way ANOVA and Duncan test. Results: There was a significant difference in abrasion of composites with different filler size (P < 0.05. The most amount of abrasion was observed in Z100 after being bleached. An increase in abrasion was noticed in all three types of tested composite after bleaching. Conclusion: According to the findings, it is suggested to use a nano filled resin composite for restoration if the bleaching treatment is required.

  1. The investigation on the cervical lesions in the combined use of cytology and the significance of hu-man papilloma virus detection%探讨子宫颈病变中联合应用细胞学及人类乳头状瘤病毒检测的意义

    Institute of Scientific and Technical Information of China (English)

    刘禄; 杨娜; 杨槐; 蒲泽宴; 刘方久; 张薇珊; 崔丽娟; 罗启翅

    2016-01-01

    目的:研究子宫颈病变诊断中联合细胞学和人类乳头状瘤病毒检测对病理诊断的作用。方法:收集遂宁市中心医院病理科子宫颈活组织检查标本876例,分别进行细胞学检查及人类乳头状瘤病毒检测,采用统计学软件进行数据分析。结果:31~50岁为高危型 HPV 感染高发年龄,分别占26.7%和45.7%。子宫颈鳞状细胞癌在41~50岁年龄组占43.7%。结论:子宫颈脱落细胞学及高危型 HPV 检测在子宫颈癌筛查及诊断中具有重要作用,随着我国经济社会的发展,国家应加大筛查的宣传力度,子宫颈脱落细胞学及高危型 HPV 检测可以明显降低子宫颈癌的发病率。%Objective:To research joint cytological diagnosis of cervical lesions and human papilloma virus detection of pathological diagnosis. Methods:Collection of suining city central hospital,876 cases of cervical biopsy specimens,respectively for cytologic examination and detection of human papilloma virus using statistical software for data analysis. Results:31 to 50 years old in high - risk type HPV infection high - risk age,respectively,26. 7% ,45. 7% . Cervical squamous cell carcinoma (43. 7% )as the highest in group C. Conclusion:Type cervical exfoliated cytology and high - risk HPV detection in cervical cancer screening and diagnosis plays an important role,along with the development of China's economic and social,countries should step up the propaganda screening,cervical exfoliated cytology and high - risk type HPV testing can significantly reduce the incidence of cervical cancer.

  2. Liquid abrasive pressure pot scoping tests report

    International Nuclear Information System (INIS)

    The primary initiatives of the LITCO Decontamination Development group at the Idaho Chemical Process Plant (ICPP) are the development of methods to eliminate the use of sodium bearing decontamination chemicals and minimization of the amount of secondary waste generated during decontamination activities. In July of 1994, a Commerce Business Daily (CBD) announcement was issued by the INEL to determine commercial interest in the development of an in-situ liquid abrasive grit blasting system. As a result of the CBD announcement, Klieber ampersand Schulz issued an Expression of Interest letter which stated they would be interested in testing a prototype Liquid Abrasive Pressure Pot (LAPP). LITCO's Decontamination group and Kleiber ampersand Schulz entered into a Cooperative Research and Development Agreement (CRADA) in which the Decontamination Development group tested the prototype LAPP in a non-radioactive hot cell mockup. Test results are provided

  3. Histomorphological Pattern of Cervical Lymphadenopathy

    Directory of Open Access Journals (Sweden)

    Abdul Mannan Sikder

    2013-01-01

    Full Text Available Background: Enlarged palpable cervical lymph nodes as a primary presenting sign are very common and may be due to inflammatory lesions and tumors. Correlation between clinical findings and laboratory data is essential in arriving at a diagnosis. In patients presenting with cervical lymphadenopathy, excision biopsy provides material to establish an early diagnosis. We designed this study in our population for histological evaluation of cervical lymph node biopsies that might be important in the management of these patients. Objective: Histopathological evaluation of different diseases involving the cervical lymph nodes in relation to age and sex of the study population. Materials and Methods: It was a cross sectional study conducted in the department of Pathology, Enam Medical College & Hospital, Savar, Dhaka during the period from January 2006 to December 2010. A total of 107 patients were evaluated for specific cause of cervical lymphadenopathy in relation to age and sex. Lymph node biopsies of all patients of both sexes and all age groups were included in the study. Results: Among the 107 subjects 58 (54.2% were males and 49 (45.8% were females with a male to female ratio of 1.2:1. The age of the patients ranged from 2 to 85 years with a mean age of 32.68 ± 18.01 years. Of the 107 lymph node biopsies, 34 cases (31.8% were reactive lymphadenitis, 41 cases (38.3% were tuberculosis, 2 cases (1.9% were non-caseous granuloma, 6 cases (5.6% were Hodgkin lymphoma, 8 cases (7.5% were non-Hodgkin lymphoma, 12 cases (11.2% were metastatic neoplasm and 4 cases (3.7% were other specific lesions. Conclusion: The commonest cause of cervical lymphadenopathy was tuberculosis, followed by reactive lymphadenitis, lymphoma and metastatic neoplasm.

  4. Patient preference: conventional rotary handpieces or air abrasion for cavity preparation.

    Science.gov (United States)

    Malmström, Hans S; Chaves, Yvette; Moss, Mark E

    2003-01-01

    It has been suggested that patients should accept the use of the air abrasion technique over the conventional handpiece due to the reduced need for anesthesia. Technologies for both air abrasion and the conventional rotary handpiece have, in recent decades, seen major improvements, but there are no recent scientific publications that evaluate the patient's preference for these two technologies when performing a cavity preparation. This study determined the patient's preference for air abrasion or the rotary handpiece for removing occlusal fissure carious lesions in mandibular premolars. Ten healthy subjects 18 years of age or older were recruited from the General Dentistry Clinic of the University of Rochester Eastman Dental Center, with fissure caries at a DEJ depth of similar size (determined by radiographs and clinical examination) in any two mandibular premolars in opposite quadrants. Within each subject, the two methods of caries removal were randomly assigned. In one premolar, air abrasion was used for cavity preparation, and in the other premolar, a conventional rotary handpiece was used. At each visit prior to treatment, the patients were instructed to complete the Emotional Status (ES) questionnaire (SUNY University at Buffalo Craniofacial Pain Clinic) to assess differences in their emotional status between appointments. At each appointment, when the restorative treatment was completed, patients were instructed to rate their pain on the Visual Analogue Scale (VAS). A technique preference questionnaire was given after the second appointment. The subjects rated the perception of pain as significantly lower (p cavity preparation when removing fissural caries in mandibular premolars and most subjects did not experience any pain when air abrasion was used.

  5. Performance Enhancement of Abrasive Waterjet Cutting

    OpenAIRE

    Vu Ngoc Pi

    2008-01-01

    Abrasive Waterjet (AWJ) Machining is a recent non-traditional machining process. This technology is widely used in industry for cutting difficult-to-machine-materials, milling slots, polishing hard materials etc. AWJ machining has many advantages, e.g. it can cut net-shape parts, no heat is generated during the cutting process, it is particularly environmentally friendly as it is clean and it does not create dust. Although AWJ machining has many advantages, a big disadvantage of this technolo...

  6. Pediatric Upper Cervical Spine Giant Cell Tumor: Case Report

    OpenAIRE

    Alfawareh, Mohammad D.; Shah, Irfanullah D.; Orief, Tamer I.; Halawani, Mohammad M.; Attia, Walid I.; Almusrea, Khaled N.

    2014-01-01

    Study Design Case report. Objective The purpose of this work is to report the case of a giant cell tumor involving the second cervical vertebra in a pediatric patient. Surgical management included a combined posterior and anterior cervical approach. There has been no recurrence in 2 years of follow-up. Case Report A 13-year-old girl presented with scoliosis with incidentally lytic lesion involving the second cervical vertebra. The radiologic investigations and biopsy result indicated a giant ...

  7. Evalution of gene expression of the human telomerase gene in the screating of cervical lesions%hTERC基因表达在宫颈病变筛查中的意义

    Institute of Scientific and Technical Information of China (English)

    周波; 李婷; 周晨; 陈忠东; 谢宛玉

    2011-01-01

    Objective: To investigate the human telomerase RNA (hTERC) gene in the screating of cervical lesions. Methods; Hie expression of hTERC gene of cervix cytologic samples were detectded by using fluorescence in situ hybridization ( FISH) . According to histology biopsy 115 Pap smears were divided into cervinl intraepithelial neoplasin ( CIN, n =69), squamous carcinomal of the cervix (SCC, n=26) . Normnl (n =20) . Results: The positive rate of hTERC gene in CIN I , CIN Ⅱ, CIN Ⅲand SCC were respectively 22. 00% , 75. 00% , 100.00%and 100. 00% (P <0.05) . hTERC gene copy numbers in CINIⅡ/CINⅢ/SCC were significantly higher than that in CIN I. The number of abnormal cells were increased in gene amplification for hTERC , CIN I was 6.50% , CINⅡ was 25. 50% , CIN Ⅲ was 30. 60% , SCC was 50. 80% (P <0.05) . The abnormal hTERC signal types of 2:3 founded in CIN I was 60.00% , in CIN Ⅱ, CIN Ⅲ and SCC were 47.10% , 45.18% and 36.44% respectively. In SCC, the abnormal hTERC signal types were 4:4 and 5 : 5 mainly. Conclusion: The increase of hTERC expression in the invasive CIN and SCC, the copy numbers are associated with the severity of cytologic and histologic findings. Therefore can as a screening detection index of biometrical genetics for progress of cervical anterior lesions.%目的:探讨人端粒酶RNA (hTERC)基因在宫颈病变筛查中的临床意义.方法:收集2008年1月~11月南华大学第一附属医院115例妇女宫颈脱落细胞标本,病理学检查确诊CIN患者69例,宫颈鳞癌(SCC)患者26例,正常细胞学妇女20例.用荧光原位杂交(FISH)方法检测脱落细胞hTERC基因的表达.结果:①hTERC基因在CIN Ⅰ、CINⅡ、CINⅢ及SCC患者宫颈脱落细胞中的阳性表达率分别是22.00%、75.00%、100.00%和100.00%.CIN Ⅰ、CINⅡ、CINⅢ和SCC组与正常组比较,hTERC基因阳性率差异有统计学意义(P<0.05).其中CIN Ⅰ与CINⅡ、CINⅢ比较,CIN Ⅰ与SCC比较差异有统计学意义(P<0

  8. 浙江沿海地区宫颈病变患者中高危型HPV检测及其临床价值%Value of high risk human papilloma virus detection in screening and diagnosing cervical lesion in littoral of Zhejiang province

    Institute of Scientific and Technical Information of China (English)

    朱军; 林颖; 阮海波; 张霞晖; 杨倩

    2013-01-01

    Objective To analyse the infection of high-risk human papiliomavirus(HR-HPV) in cervical lesion wome,and evaluate the significance of high-risk human pappilomavirus detection by hybrid capture Ⅱ (HV-Ⅱ) in screening and diagnosing cervical lesion,especially high grade cervical intraepithelial neoplasia(CIN).Methods A series of 1130 patients of cervical lesion were preliminarily diagnosed by cervical cytological examination,HR-HPV detection by HC-Ⅱ,colposcopy and biopsy under the colposcopy between June 2009 and December 2008,including 212 CIN Ⅰ and(or)condyloma(CIN Ⅰ/HPV Ⅰ),442 CIN Ⅱ/Ⅲ,28 invasive cervical cancer.cervical cytological examination is by thin prep liquid-based cytology test(TCT),and HR-HPV detection is by HC-Ⅱ.Results In 1130 cases the positive of HR-HPV was 65.84% (744/1130).Unusual cytology result were 862 cases,with 356 ASCUS,84 ASCH,216 LSIL,184HSIL and 22 cancer.The number of biopsy ≥ CINI/HPVI was 682,positive rate of HR-HPV was 78.59% (536/682).In screening CIN Ⅱ or above,sensitivity,specificity,PPV and NPV of TCT were 88.94%,32.73%,48.49%,80.60%,ofHR-HPV DNA detectiort by HC-Ⅱ were 90.21%,51.82%,57.14%,88.14%,and of HR-HPV detection combined with cytology were 97.45%,22.42%,47.22%,92.50%.Conclusion The infection rate of HR-HPV in cervical lesions is higher in each age group.Infection rate of HR-HPV is ascending with serious degree of cervical lesion.HR-HPV detection by HC-Ⅱ is an important method in screening cervical lesion.HR-HPV detection is a viable option in the management of women with ASCUS and LSIL of TCT,with higher sensitivity and NPV.%目的 分析宫颈病变患者中高危型HPV的感染情况及特点,探讨HR-HPV DNA检测用于高级别宫颈上皮内瘤变中的价值.方法 回顾性分析采用液基薄层细胞学(TCT)检查、HR-HPV检测和阴道镜检查并行活检的官颈病变患者1130例临床资料.经病理组织学检查证实宫颈炎症448例,官颈上皮

  9. Langerhans cell histiocytosis of the cervical spine: case report of an unusual location

    Energy Technology Data Exchange (ETDEWEB)

    Geusens, E.; Brys, P.; Ghekiere, J.; Baert, A.L. [Department of Radiology, University Hospital Gasthuisberg KU Leuven (Belgium); Samson, I. [Department of Orthopedic Surgery, University Hospitals, Leuven (Belgium); Sciot, R. [Department of Pathology II, University Hospitals, Leuven (Belgium); Brock, P. [Department of Pediatrics, University Hospitals, Leuven (Belgium)

    1998-09-01

    An unusual location for Langerhans cell histiocytosis of the cervical spine is presented. The osteolytic lesion, instead of being located in the vertebral body, was visualised in the left lateral mass of the fifth cervical vertebra, extending into the vertebral body and through the interapophyseal joint into the lateral mass of the fourth cervical vertebra. (orig.) With 3 figs., 7 refs.

  10. Langerhans cell histiocytosis of the cervical spine: case report of an unusual location

    International Nuclear Information System (INIS)

    An unusual location for Langerhans cell histiocytosis of the cervical spine is presented. The osteolytic lesion, instead of being located in the vertebral body, was visualised in the left lateral mass of the fifth cervical vertebra, extending into the vertebral body and through the interapophyseal joint into the lateral mass of the fourth cervical vertebra. (orig.)

  11. Langerhans cell histiocytosis of the cervical spine: case report of an unusual location.

    Science.gov (United States)

    Geusens, E; Brys, P; Ghekiere, J; Samson, I; Sciot, R; Brock, P; Baert, A L

    1998-01-01

    An unusual location for Langerhans cell histiocytosis of the cervical spine is presented. The osteolytic lesion, instead of being located in the vertebral body, was visualised in the left lateral mass of the fifth cervical vertebra, extending into the vertebral body and through the interapophyseal joint into the lateral mass of the fourth cervical vertebra. PMID:9724427

  12. 高频电刀与复方甲硝唑栓联合治疗宫颈变的疗效观察%The efficacy of high-frequency electrotome combined with compound metronidazole suppositories in the treatment of cervical lesions

    Institute of Scientific and Technical Information of China (English)

    李婉玲

    2013-01-01

    Objectives To explore the efficacy of high-frequency electrotome circumcision or electrocoagulation combined with compound metronidazole suppositories in the treatment of cervical lesions.Methods 200 patients with cervical lesions were randomly divided into a study group and a control group.The study group received high-frequency electrotome circumcision or electrocoagulation combined with compound metronidazole suppositories; the control group received high-frequency electrotome circumcision or electrocoagulation alone.Results The duration of vaginal discharge was shorter in the study group than in the control group (P<0.01).The rates of postoperative wound infection and cervical contact bleeding was obviously lower in the study group than in the control group (P<0.05).The recovery of cervical canal shape did not differ significantly between the two groups (P>0.05).No cervical stenosis occurred in both groups.Conclusions High-frequency electrotome circumcision or electrocoagulation combined with compound metronidazole suppositories can obviously shorten duration of vaginal discharge and reduce incidence of complications.%目的 观察高频电刀环切术或电凝术联合复方甲硝唑栓治疗宫颈病变的临床疗效.方法 选择200例宫颈病变的患者,随机分为研究组和对照组,研究组应用高频电刀环切术或电凝术联合复方甲硝唑栓治疗宫颈病变;对照组单纯使用高频电刀环切术或电凝术治疗宫颈病变.结果 与对照组比较,研究组患者的术后阴道排液持续天数明显缩短(P<0.01),术后创面感染、宫颈接触性出血等并发症发生率明显降低(P<0.05),宫颈管形态恢复上两组无明显差异(P>0.05),均无发生宫颈狭窄.结论 高频电刀环切术或电凝术联合复方甲硝唑栓治疗宫颈病变可以明显减少术后阴道排液天数以及并发症的发生.

  13. Generalized cervical root resorption associated with periodontal disease

    NARCIS (Netherlands)

    Beertsen, W; Piscaer, M; Van Winkelhoff, AJ; Everts, P. A. M.

    2001-01-01

    Background and description of case: The etiology and pathogenesis of generalized cervical root resorptions is not well understood. In the present report, a case of severe cervical root resorption involving 24 anterior and posterior teeth is presented. The lesions developed within a period of 2 years

  14. Gene promoter methylation patterns throughout the process of cervical carcinogenesis

    NARCIS (Netherlands)

    Yang, Nan; Nijhuis, Esther R.; Volders, Haukeline H.; Eijsink, Jasper J. H.; Lendvai, Agnes; Zhang, Bo; Hollema, Harry; Schuuring, Ed; Wisman, G. Bea A.; van der Zee, Ate G. J.

    2010-01-01

    Objectives: To determine methylation status of nine genes, previously described to be frequently methylated in cervical cancer, in squamous intraepithelial lesions (SIL). Methods: QMSP was performed in normal cervix, low-grade ( L) SIL, high-grade (H) SIL, adenocarcinomas and squamous cell cervical

  15. Liquid Based Cytology and Colposcopy in the Screening of Cervical Cancer and Precancerous Lesions%宫颈液基细胞学及阴道镜在宫颈癌及癌前病变中的筛查作用

    Institute of Scientific and Technical Information of China (English)

    王清; 胡霞

    2015-01-01

    目的:分析液基细胞学和阴道镜在宫颈癌及癌前病变中的筛查作用,评估液基细胞学和阴道镜在宫颈癌及癌前病变筛查中的临床应用价值。方法回顾性分析2013年5月-2014年8月接受宫颈癌筛查的500例女性的临床资料,所有体检者都接受阴道镜检查和液基细胞学检查。结果阴道镜检测假阳性患者有11例,阴道镜检测的特异性为97.39%(412/423),漏诊率为1.00%(5/500);液基细胞学诊断的假阳性患者有15例,由检测结果可知液基细胞学检测宫颈癌的检测特异性为96.45%(408/423),漏诊率为6.40%(32/500)。结论液基细胞学和阴道镜在宫颈癌及癌前病变筛查中具有临床应用价值,阴道镜对宫颈癌及癌前病变的检出率较液基细胞学高。%Objective To study the application value of liquid based cytology and colposcopy in the screening of cervical cancer and precancerous lesions .Methods Retrospective analysis of clinical data of 500 female patients who underwent cervical cancer screening were conducted .All subjects received colposcopy and liquid based cytology .Results Colposcopy results showed that false positive were 11 cases,the specificity of colposcopy was 97.39%(412 /423),the rate of missed diagnosis was 1.00%(5 /500);liquid based cytology diagnosis showed that false positive were 15 cases,specificity was 96.45%(408/423),the rate of missed diagnosis was 6.40%(32 /500).Conclusion Liquid based cytology and colposcopy has clinical application value in the screening of cervical cancer and precancerous lesions ,Colposcopy for cervical cancer and precancerous lesions has higher detec -tion rate compared with liquid based cytology .

  16. Implications of tyrosine phosphoproteomics in cervical carcinogenesis

    Directory of Open Access Journals (Sweden)

    DeFord James

    2008-01-01

    Full Text Available Abstract Background Worldwide cervical cancer remains a leading cause of mortality from gynecologic malignancies. The link between cervical cancer and persistent infection with HPV has been established. At a molecular level little is known about the transition from the precancerous state to invasive cancer. To elucidate this process, cervical biopsies from human specimens were obtained from precancerous state to stage III disease. Methods Cervical biopsies were obtained from patients with a diagnosis of cervical cancer undergoing definitive surgery or staging operation. Biopsies were obtained from patients with precancerous lesions at the time of their excisional procedure. Control samples were obtained from patients undergoing hysterectomy for benign conditions such as fibroids. Samples were subjected to proteomic profiling using two dimensional gel electrophoresis with subsequent trypsin digestion followed by MALDI-TOF protein identification. Candidate proteins were then further studied using western blotting, immunoprecipitation and immunohistochemistry. Results Annexin A1 and DNA-PKcs were found to be differentially expressed. Phosphorylated annexin A1 was up regulated in diseased states in comparison to control and its level was strongly detected in the serum of cervical cancer patients compared to controls. DNA-PKcs was noted to be hyperphosphorylated and fragmented in cancer when compared to controls. By immunohistochemistry annexin A1 was noted in the vascular environment in cancer and certain precancerous samples. Conclusion This study suggests a probable role for protein tyrosine phosphorylation in cervical carcinogenesis. Annexin A1 and DNA-PK cs may have synergistic effects with HPV infection. Precancerous lesions that may progress to cervical cancer may be differentiated from lesions that will not base on similar immunohistochemical profile to invasive squamous cell carcinoma.

  17. Aberrant cervical thymus mimicking thyroid on ultrasonography: A case report

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Jeong Sub; Park, Ju Hyun; Kim, Bong Soo; Park, Ji Kang; Choi, Jae Hyuck [Jeju National Univ. Hospital/Jeju National Univ. School of Medicine, Jeju (Korea, Republic of)

    2012-10-15

    Aberrant cervical thymus is rarely reported in adults. We report a case of solid aberrant cervical thymus in a 27 year old female, which was found incidentally on ultrasonography for the evaluation of the thyroid cancer. On ultrasonography, the lesion was found between the left thyroid and common carotid artery without any remarkable interface echo, and had similar echogenicity to the thyroid. The lesion extended to the upper pole of the left thyroid.

  18. Diagnosis value of liquid-based cytology and conventional cytology smears on detecting cervical squamous cell lesions%液基细胞学技术与传统细胞学涂片技术对宫颈病变细胞学的诊断价值

    Institute of Scientific and Technical Information of China (English)

    董彦军; 郑加荣

    2013-01-01

    Objective To compare the value of liquid-based cytology and conventional cytology smears on detection of cervical squamous cell lesions.Methods One thousand and two hundred women were detected by cervical liquid-based cytology and conventional cytology smear screening from Oct.2009 to Oct.2012,of which positive cases were detected by colposcopy and biopsy as the gold standard as a positive control.The diagnostic value of the liquid-based cytology on cervical lesions was assessed.Results The positive detection rate of ASCUS/A-GUS and above lesions was 16.00% (96 cases) by liquid-based cytology detection,and positive detection rate of pap ⅡB level and above lesions was 4.83 % (29 cases).The positive detection rate by liquid-based cytology was significantly higher than that of conventional cytology smears,the difference was statistically significant (P < 0.05).Colposcopic and histopathological findings as the gold standard,cervical liquid-based cytology screening coincidence rate was 76.80%,conventional cytology smear screening coincidence rate was 38.16%,and the difference was statistically significant (P < 0.05).Conclusions Liquid-based cytology screening for cervical squamous cell lesions is significantly superior to conventional cytology smears.%目的 比较液基细胞学技术与传统细胞学涂片在宫颈鳞状细胞病变检测中的价值.方法 对2009年10月至2012年10月1200例妇女采用宫颈液基细胞学或传统细胞学涂片进行筛查,并对筛查阳性病例以其阴道镜检查及病理活检结果为金标准作为阳性对照,评估液基细胞学技术对宫颈不同病变的诊断价值.结果 宫颈液基细胞学筛查出ASCUS/A-GUS及以上分级病例96例,阳性检出率为16.00%;传统细胞学涂片筛查出巴氏ⅡB级及以上分级病例29例,阳性检出率为4.83%;宫颈液基细胞学阳性检出率明显高于传统细胞学涂片,差异有统计学意义(P<0.05).以阴道镜及病理组织学

  19. Endometrial cancer with cervical extension mimicking dual concordant endometrial and cervical malignancy by F18 FDG PET and MRI

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Seok Nam [Kwandong Univ. College of Medicine, Seoul (Korea, Republic of)

    2012-09-15

    A 35 year old woman with endometrial cancer and cervical extension underwent F18 FDG PET CT and MRI studies after resection of a cervical mass presumed to be cervical myoma. The patient underwent cervical myomectomy and the histopathologic report revealed poorly differentiated invasive carcinoma. Cervical cancer was ruled out because the patient had no history of sexual intercourse and was negative for human papilloma virus infection. The patient underwent radical hysterectomy, bilateral salpingo oophorectomy, pelvic and para aortic lymph node dissection, and multiple biopsies. F18 FDG PET CT showed intense FDG uptake along the cervix wall. T2 weighted MRI also revealed a mass lesion with high SI involving the anterior and posterior lips of the uterine cervix. Another area of focal increased uptake above the endometrial lesion in the left pelvic cavity was observed on PET CT and MRI, possibly due to a functioning ovary. PET CT and MRI were interpreted as showing a dual concordant malignant lesion due to separated FDG uptakes and high SI without any connection between the cervical and endometrial lesions. F18 FDG PET CT showed intense FDG uptake along the endometrium. Given the patient's history and the fact that she was not menstruating at the time of imaging, this intense uptake was interpreted as another pathologic lesion, suggesting dual primary lesions. A suspected heterogeneous mass lesion along the endometrium suggesting concordant endometrial cancer was found on MRI. Endometrial cancer with cervical extension is sometimes difficult to differentiate from primary cervical cancer. The final histopathologic report showed poorly differentiated endometrial adenocarcinoma with cervical extension, although the FDG PET CT and MRI findings were suggestive of concordant cervical and endometrial cancer. Although histopathologic confirmation is necessary for final diagnosis, MRI and FDG PET CT studies may aid in the differential diagnosis. A metastatic cervical mass

  20. Distracted cervical spinal fusion for management of caudal cervical spondylomyelopathy in large-breed dogs

    International Nuclear Information System (INIS)

    Using an autogenous bone graft (obtained from the iliac crest), 4-mm cancellous bone screws, and polymethylmethacrylate, a distracted cervical spinal fusion technique was performed on 10 dogs with myelographic evidence of caudal cervical spondylomyelopathy. All dogs had evidence of dynamic soft tissue spinal cord compression, as indicated by flexion, extension, and traction myelographic views. Of the 10 dogs, 4 previously had undergone surgery by use of ventral slot or cervical disk fenestration techniques, and their neurologic status had deteriorated after the original surgery. Preoperative neurologic status of the 10 dogs included nonambulatory tetraparesis (n = 5), severe ataxia with conscious proprioceptive deficits (n = 2), and mild ambulatory ataxia with conscious proprioceptive deficits (n = 3). Five dogs had signs of various degrees of cervical pain. Clinical improvement was observed in 8 of 10 dogs--either improved neurologic status or elimination of cervical pain. Implant loosening developed in 3 dogs; 2 of them were euthanatized because of lack of neurologic improvement. Radiographic evidence of bony cervical fusion was observed during a 9- to 24-week period in 6 of the 8 surviving dogs. The distracted cervical fusion technique appears to be a valid surgical procedure to manage cervical spondylomyelopathy in those dogs in which the lesions are limited to one cervical intervertebral disk space

  1. p16及pRb在宫颈癌中的表达及意义%Immunochemical analysis of p16 and pRb in uterine cervical lesions

    Institute of Scientific and Technical Information of China (English)

    武海英; 魏利; 史惠蓉

    2011-01-01

    Objective To detect the expression of pi6 and pRb in cervical intraepithelial neoplasia and their association with cervical cancers. Methods Human papilloma virus was detected with surface plasmon resonance technique, and the expressions of P16 and pRb were examined with immunohistochemistry technique in 108 cases of CIN I , 50 cases of CIN H ? 35 cases of CINIH and 52 cases of cervical carcinomas. Results The positive rate of human papilloma virus and PI6 showed increasing tendency, and the positive rate of pRb showed decreasing tendency in CIN I , CINFJ ? CIN UI , cervical squamous carcinoma and cervical adenocarcinoma. The expression of PI6 and human papilloma virus were negatively correlated with the expression of pRb(r= -0. 537,Pcervical cancer. The expression of pi6 would be an early indicator for cervical cancer.%目的 探讨人乳头瘤病毒、p16及pRb在宫颈癌的表达及其相关性.方法 宫颈上皮内瘤样变(cervical intraepithelial neoplasia,CIN)I型患者108例,CINⅡ型患者50例,CINⅢ型患者35例及宫颈癌患者52例,应用表面等离子体谐振技术、免疫组织化学方法检测人乳头瘤病毒P16及pRb的表达情况.结果 从CIN到宫颈癌患者人乳头瘤病毒、P16阳性率呈逐渐增高趋势,pRb阳性率呈逐渐下降趋势;P16表达与pRb表达呈负相关(r=-0.537,P<0.01),人乳头瘤病毒与pRb表达呈负相关(r=-0.513,P<0.05),人乳头瘤病毒与P16表达呈正相关(r=0.815,P<0.05).结论 人乳头瘤病毒感染是宫颈癌发生的主要原因,p16可作为宫颈癌的早期预测指标.

  2. Tangental Turning of Incoloy Alloy 925 Using Abrasive Water Jet

    OpenAIRE

    Cárach, J.; S. Hloch; Hlaváček, P.; K. Vasilko; Lehocká, D.

    2015-01-01

    The paper deals with tangential turning of Incoloy alloy 925 with the diameter 50mm using the abrasive water jet. Experiment was done using the abrasive water jet of pressure p=400MPa and traverse speed at levels of v=1,5;3;4,5;6;7,5;9mm min-1. the abrasive particles were feeded to the water jet in the amount of 400 g min-1. Revolution of Incoloy workpiece during turning was n=34rpm.

  3. Abrasion Resistance Comparison between Rotor and Ring Spun Yarn

    Institute of Scientific and Technical Information of China (English)

    YANG Jian-ping; YU Chong-wen

    2002-01-01

    On the base of literature review and the analysis of yarn properties, yarn structure and some other facts, the abrasion resistance of both rotor spun yarn and ring spun yarns are discussed. The results show that with the same raw material and twist, the rotor spun yarn has lower abrasion resistance than that of ring spun yarn, because of the higher twist employed, the abrasion resistance of rotor spun yarn is higher than that of ring spun yarn.

  4. A new dimension to conservative dentistry: Air abrasion

    Directory of Open Access Journals (Sweden)

    Hegde Vivek

    2010-01-01

    Full Text Available Air abrasion dentistry has evolved over a period of time from a new concept of an alternative means of cavity preparation to an essential means of providing a truly conservative preparation for preservation of a maximal sound tooth structure. The development of bonded restorations in combination with air abrasion dentistry provides a truly minimal intervention dentistry. This article reviews the development of air abrasion, its clinical uses, and the essential accessories required for its use.

  5. An interdisciplinary approach for the management of noncarious lesions.

    Science.gov (United States)

    Purushotham, Sahana; Manjunath, Nandini; D'Souza, Melba Lisa; Shetty, Roshan

    2016-01-01

    Miller's class III gingival recession is a common entity observed in individuals, posing a difficult situation for the clinicians to treat. Large fenestration defects with cervical abrasion compromise the esthetics resulting in poor prognosis. Obtaining predictable and esthetic root coverage has become an integral part of periodontal therapy. The present case report describes a situation where class III gingival recession with cervical abrasion was seen in the lower central incisors. The patient was successfully treated with a combination of restorations (Filtek Z350(®) composite resin) to fill the defect, followed by fenestration procedure to increase the vestibular depth and subsequently with grafting procedure (soft tissue autograft with bovine-derived xenograft collagen [Bio-oss]). Though the results were not tangible esthetically, it was functionally successful as evidenced during the follow-up period. PMID:27143838

  6. An interdisciplinary approach for the management of noncarious lesions

    Directory of Open Access Journals (Sweden)

    Sahana Purushotham

    2016-01-01

    Full Text Available Miller's class III gingival recession is a common entity observed in individuals, posing a difficult situation for the clinicians to treat. Large fenestration defects with cervical abrasion compromise the esthetics resulting in poor prognosis. Obtaining predictable and esthetic root coverage has become an integral part of periodontal therapy. The present case report describes a situation where class III gingival recession with cervical abrasion was seen in the lower central incisors. The patient was successfully treated with a combination of restorations (Filtek Z350® composite resin to fill the defect, followed by fenestration procedure to increase the vestibular depth and subsequently with grafting procedure (soft tissue autograft with bovine-derived xenograft collagen [Bio-oss]. Though the results were not tangible esthetically, it was functionally successful as evidenced during the follow-up period.

  7. Positive diagnostic values and histological detection ratios from the Rotterdam cervical cancer screening programme

    NARCIS (Netherlands)

    F.A. Kreuger; H. Beerman (Henk); H.G. Nijs (Huub); M. van Ballegooijen (Marjolein)

    1998-01-01

    textabstractBACKGROUND: In organized screening programmes for cervical cancer, pre-cancerous lesions are detected by cervical smears. However, during follow-up after a positive smear these pre-cancerous lesions are not always found. The purpose of the study is to analys

  8. Abrasive water jet: a complementary tool

    Directory of Open Access Journals (Sweden)

    Duarte, J. P.

    1998-04-01

    Full Text Available The abrasive water jet is a powerful cutting tool, whose main advantages lie in the absence of thermal effects and the capability of cutting highly thick materials. Compared with Laser, the abrasive water jet allows the cutting of a larger range of thicknesses and a wider variety of materials such as: ornamental stones, metals, polymers, composites, wood, glass and ceramics. The application of this technology has suffered an extensive growth, with successful applications in varied industrial sectors like the automotive, aerospace, textile, metalworking, ornamental stones, etc. The present communication aims at introducing the abrasive water jet as a complementary tool to laser cutting, presenting its advantages by showing some documented examples of pieces cut for different industries.

    O jacto de água abrasivo é uma poderosa ferramenta de corte, tendo como principais vantagens a ausência de processo térmico e permitir o corte de elevadas espessuras. Comparativamente com o laser o jacto de água abrasivo permite cortar uma maior gama de espessuras, e uma maior diversidade de materiais: rochas ornamentais, metais, polimeros, compósitos, madeiras, vidro e cerâmicos. A aplicação desta tecnologia tem sofrido um crescimento acentuado, existindo aplicações de sucesso nos mais variados sectores industriáis como a indústria automóvel, aeroespacial, têxtil, metalomecânica e rochas ornamentáis. Esta comunição pretende apresentar o corte por jacto de agua abrasivo como uma ferramenta de corte complementar ao corte por laser, apresentando as suas vantagens documentadas através de alguns exemplos de peças executadas para as diferentes indústrias.

  9. Machining human dentin by abrasive water jet drilling.

    Science.gov (United States)

    Kohorst, Philipp; Tegtmeyer, Sven; Biskup, Christian; Bach, Friedrich-Wilhelm; Stiesch, Meike

    2014-01-01

    The aim of this experimental in-vitro study was to investigate the machining of human dentin using an abrasive water jet and to evaluate the influence of different abrasives and water pressures on the removal rate. Seventy-two human teeth had been collected after extraction and randomly divided into six homogeneous groups (n=12). The teeth were processed in the area of root dentin with an industrial water jet device. Different abrasives (saccharose, sorbitol, xylitol) and water pressures (15 or 25 MPa) were used in each group. Dimensions of dentin removal were analysed using a stripe projection microscope and both drilling depth as well as volume of abrasion were recorded. Morphological analyses of the dentin cavities were performed using scanning electron microscopy (SEM). Both drilling depth and volume of abrasion were significantly influenced by the abrasive and the water pressure. Depending on these parameters, the drilling depth averaged between 142 and 378 μm; the volume of abrasion averaged between 0.07 and 0.15 mm3. Microscopic images revealed that all cavities are spherical and with clearly defined margins. Slight differences between the abrasives were found with respect to the microroughness of the surface of the cavities. The results indicate that abrasive water jet machining is a promising technique for processing human dentin. PMID:24642975

  10. Machining human dentin by abrasive water jet drilling.

    Science.gov (United States)

    Kohorst, Philipp; Tegtmeyer, Sven; Biskup, Christian; Bach, Friedrich-Wilhelm; Stiesch, Meike

    2014-01-01

    The aim of this experimental in-vitro study was to investigate the machining of human dentin using an abrasive water jet and to evaluate the influence of different abrasives and water pressures on the removal rate. Seventy-two human teeth had been collected after extraction and randomly divided into six homogeneous groups (n=12). The teeth were processed in the area of root dentin with an industrial water jet device. Different abrasives (saccharose, sorbitol, xylitol) and water pressures (15 or 25 MPa) were used in each group. Dimensions of dentin removal were analysed using a stripe projection microscope and both drilling depth as well as volume of abrasion were recorded. Morphological analyses of the dentin cavities were performed using scanning electron microscopy (SEM). Both drilling depth and volume of abrasion were significantly influenced by the abrasive and the water pressure. Depending on these parameters, the drilling depth averaged between 142 and 378 μm; the volume of abrasion averaged between 0.07 and 0.15 mm3. Microscopic images revealed that all cavities are spherical and with clearly defined margins. Slight differences between the abrasives were found with respect to the microroughness of the surface of the cavities. The results indicate that abrasive water jet machining is a promising technique for processing human dentin.

  11. The Abrasion-resistance Investigation of Rubberized Concrete

    Institute of Scientific and Technical Information of China (English)

    KANG Jingfu; ZHANG Bo; LI Guangyu

    2012-01-01

    The abrasion resistance properties of rubberized concrete were comparatively studied by taking silica fume and crumb tire rubber as the additives.The abrasion tests were conducted in accordance with the Chinese standard test method DL/T 5150-2001,two recommended test methods:under water method and ring method,were used.The crumb tire rubbers with the sieve size of 8-mesh and 16-mesh were incorporated into the concrete by replacing same volume of sand and as an additive.The abrasion resistance of concrete was evaluated according to the abrasion resistance strength and the mass loss.Test results show that the addition of silica fume enhanced both compressive strength and abrasion resistance of concrete,and the addition of crumb rubber reduced the compressive strength but increased notably the abrasion resistance of the concrete.Silica fume concrete performed a better abrasion resistance than control concrete,and the rubberized concrete performed a much better abrasion resistance than silica fume concrete.The abrasion resistance of rubberized concrete increased with the increase of rubber content.

  12. Laminoplasty for Cervical Myelopathy

    OpenAIRE

    Ito, Manabu; Nagahama, Ken

    2012-01-01

    This article reviews cervical laminoplasty. The origin of cervical laminoplasty dates back to cervical laminectomy performed in Japan ~50 years ago. To overcome poor surgical outcomes of cervical laminectomy, many Japanese orthopedic spine surgeons devoted their lives to developing better posterior decompression procedures for the cervical spine. Thanks to the development of a high-speed surgical burr, posterior decompression procedures for the cervical spine showed vast improvement from the ...

  13. Research on Premixed Abrasive Jet Derusting Machine

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    The structure, working principles, and main technological parameters of the premajet derusting machineare introduced. Experiments were made to test the relationship among such jet parameters as working pressure,rate of water flow, abrasive weight consistency and derusting efficiency. Reasonable parameters were decided. Re-su lts prove that the derusting machine is characterized by its high derusting efficiency (as high as 20 m2/h), goodderusting quality (as good as ISO8501-1 Sa 2.5), and low specific power consumption (about 0.3 kW · h/m2).Therefore it is a new type of high efficiency derusting machine.

  14. Cervical chordoma: a case report; Cordoma cervical: a proposito de un caso

    Energy Technology Data Exchange (ETDEWEB)

    Romera, C.; Wiehoff, A.; Candela, V. P.; Perera, J. [Hospital Universitario Materno-Insular de Canarias. Las Palmas (Spain)

    2002-07-01

    Chordomas, lesions that develop from notochordal remnants, can arise at any site ranging from the clivus to the sacrum: they represent 3% to 4% of all primary bone tumors. We present the cases of a 45-year-old man with cervical chordoma at the C2 level, the site least frequently reported in the literature. We provide the radiological findings resulting from cervical computed tomography and magnetic resonance imaging. (Author) 11 refs.

  15. [Cervical radiculopathy].

    Science.gov (United States)

    Kuijper, B

    2014-10-01

    Cervical radiculopathy is a common cause of pain in the arm. It is caused by nerve root compression in the neck, as a consequence of a herniated disc, or spondyliotic foraminal stenosis. It causes severe pain, especially during the first few weeks, and paraesthesias in the forearm and hand. Patients also suffer from neck pain and loss of strength in the relevant arm. The arm pain can be exacerbated by certain movements of the head; these should be avoided as much as possible. Diagnosis can be made on the basis of history and physical examination. The pain generally disappears without active patient treatment. A semi-rigid cervical collar is recommended to accelerate pain relief. In cases of persistent pain, surgery will be considered. In such cases an MRI should be performed to show the cause and level of nerve root compression. PMID:26185991

  16. Diagnosis of cervical cancer with transvaginal color Doppler sonography

    Directory of Open Access Journals (Sweden)

    Li-bo DENG

    2011-09-01

    Full Text Available Objective To investigate the imaging features of cervical cancer by transvaginal color Doppler sonography(TVCS,and evaluate the diagnostic value of TVCS.Methods A hundred and thirty cases of cervical intraepithelial neoplasia(CIN grade Ⅰ-Ⅱ and cervical cancer,diagnosed by Thinprep cytologic test(TCT,cervical biopsy and pathological examination,received TVCS examination.The image characters and color Doppler flow imaging(CDFI were collected and analyzed.Another 41 cases with normal cervices as determined by inspection and cytological examination were involved as control.Results In order of normal cervix,CIN,cancer in situ and cervical cancer,the cervical diameter showed a tendency of increase,also with an increase incidence of low-level echo focus in cervix.As a specific image of cervical cancer,the low level echo focus occurred only in cervical cancer with a specificity of 100%.The absence of mucosal line in cervical canal was a specific character of stage Ⅱ cervical cancer with a specificity of 100%.CDFI and resistance index(RI revealed that the local blood flow was more abundant in invasive cancer than in CIN and cancer in situ,and significant difference was found between stage Ⅰ and stage Ⅱ cervical cancer(P < 0.05.The sensitivity and specificity of enlarged cervical diameters in diagnosis of cervical cancer were 89.1% and 82.8%.The specificity of cervical low level echo focus in diagnosis of cervical cancer and invasive cervical cancer were 100% and 94.8%,respectively.The specificity of abundant blood flow in dendritic form in diagnosis of invasive cervical cancer was 100%.Conclusions Invasive cervical cancer may present several specific features in TVCS images.TVCS examination is of high reliability in diagnosis of invasive cervical cancer,but is not so reliable in diagnosing precancerous lesion and preinvasive cancer.Combined with other auxiliary examinations,TVCS could be considered as one of the methods to diagnose cervical

  17. Incidence of lameness and abrasions in piglets in identical farrowing pens with four different types of floor

    Directory of Open Access Journals (Sweden)

    Nilsson Ebba

    2009-05-01

    Full Text Available Abstract Background Lameness in piglets is a major animal welfare issue. Floor abrasiveness is a common cause of superficial injury in piglets in farrowing pens. The abrasion achieved may act as a gate for infections, which in turn may induce development of infectious arthritis. In this study, the influence of improvements of the floor quality and of increased ratios of straw in identical farrowing pens was measured. Methods The study was carried out at a herd with four identical farrowing units with solid concrete floor bedded with 1 kg chopped straw per sow and 1 hg per piglet and day. Nothing was changed in the management of the four identical farrowing units, but four experimental groups were created: Group I – control, Group II – the amount of bedding was doubled. The surface of the floor was repaired in two units, Group III – Piglet Floor®, Flowcrete Sweden AB, Perstorp, Sweden and Group IV – Thorocrete SL®, Växa Halland, Sweden. Three farrowing batches were studies in each unit. In total, 93 litters (1,073 piglets were examined for foot and skin lesions until the age of 3 weeks. The occurrence of lameness was registered until weaning at an average age of 4.5 weeks. Twenty seven lame piglets were culled instead of medicinally treated and subjected to necropsy including histopathological and microbiological examinations. Isolates of streptococci, staphylococci and E. coli were tested with respect to antimicrobial resistance. Results Piglet born on the repaired floors had the lowest prevalences of abrasions at carpus. Also the doubled straw ration decreased the abrasions. Skin lesions at carpus decreased significantly in magnitude in all four systems from day 10. At day 3, the sole bruising scores of the control unit were greater than the other three units (p Streptococcus dysgalactiae subsp. equisimilis (60%, Staphylococcus hyicus subsp. hyicus (35% and Escherichia coli (5%. These isolates were sensitive to all antibiotics

  18. Impact of toothpaste slurry abrasivity and toothbrush filament stiffness on abrasion of eroded enamel - an in vitro study

    OpenAIRE

    Wiegand, Annette; Schwerzmann, Martina; Sener, Beatrice; Magalhães, Ana C.; Roos, Malgorzata; Ziebolz, Dirk; Imfeld, Thomas; Attin, Thomas

    2008-01-01

    OBJECTIVE: Toothbrush abrasion is significant in the development of tooth wear, particularly when combined with erosion. This in vitro study aimed to evaluate the impact of toothpaste slurry abrasivity and toothbrush filament stiffness on abrasion of eroded enamel. MATERIAL AND METHODS: Eroded enamel samples (hydrochloric acid, pH: 2.6, 15 s) were brushed with 40 strokes in an automatic brushing machine using manual toothbrushes with different filament stiffness (filament diameter: 0.15, 0.20...

  19. Ultrasonic Abrasion: An Alternative for Cavity Preparation

    Directory of Open Access Journals (Sweden)

    Áurea Simone Barrôso VIEIRA

    2007-05-01

    Full Text Available Introduction: Restorative dentistry aims to repair damages caused by caries disease. Along the years, researchers have developed effective and less invasive methods with the goal of preserving the teeth from caries destruction. Therefore, the improvement of scientific knowledge, auxiliary diagnostic systems, dental materials, and new instruments has changed the approaches and treatments in this field. In addition to conservative removal of carious tissue, patient’s comfort has also become a concern in modern dentistry.Purpose: Considering that ultrasonic abrasion has attracted great interest of dental professionals, this article discusses an alternative technique for cavity preparation by literature review, addressing its indications, contra-indications, advantages and limitations compared to the conventional high-speed method.Conclusion: There are not many studies on this subject. The available studies have demonstrated several qualities of the ultrasonic abrasion system, but some aspects remain unclear. Therefore, it is important to highlight that laboratorial and clinical studies in primary/permanent teeth should be conducted to elucidate questionable issues, such as time of cavity preparation, topography, presence of smear layer and microleakage, in order to offer safety to the extensive use of this new technology for both the professional and the patient. In this context, the dentist should always be attentive to innovations referring to minimally invasive techniques.

  20. [Cervical Spondylotic Amyotrophy].

    Science.gov (United States)

    Sonoo, Masahiro

    2016-05-01

    Keegan (1965) reported a patient who presented with "dissociated motor loss," an acute paralysis of the upper extremity with minimal sensory signs and no long tract signs, and documented an anterior root lesion following autopsy. Sobue et al. (1975) reported similar cases using the term "cervical spondylotic amyotrophy (CSA)," but postulated pathology of the anterior horn. Although Keegan's "dissociated motor loss" surely referred to isolated motor paresis with no or minimal sensory signs, contrary to existing criticism, a more general term, CSA, should be preferred. CSA is divided into proximal and distal types. Distal CSA often presents with a drop finger, and thus may be misdiagnosed as posterior interosseous nerve palsy. Documentation of the involvement of ulnar muscles by clinical signs and EMG would lead to the diagnosis of distal CSA. Proximal CSA may be confused with neuralgic amyotrophy (NA), although the sparing of the serratus anterior and the stereotypic involvement of deltoid, infraspinatus, biceps brachii, and brachioradialis suggest CSA. Cervical MRI is not diagnostic in around half of CSA cases, and denervation in paraspinal EMG is a more sensitive test that can exclude NA. Amyotrophic lateral sclerosis is another important differential diagnosis for CSA. PMID:27156504

  1. 高危型HPV检查和TCT在宫颈癌及癌前病变的早期诊断临床研究%Clinical studies of high-risk HPV examination and TCT in the early diagnosis of cervical cancer and precancerous lesions.

    Institute of Scientific and Technical Information of China (English)

    董静; 倪爱军

    2013-01-01

    Objective To investigate the clinical value of the high - risk types of human papillomavims ( HPV ) examination and thinprep cytologic test ( TCT ) in the early diagnosis of cervical cancer and precancerous lesions. Methods High - risk HPV and TCT examination results in 1248 women screened for cervical cancer were retrospectively analyzed. The clinical diagnostic value of high - risk HPV and TCT examination and the combination of the two methods was compared. Results High - risk HPV infection rate and TCT - positive rate in cervical intraepithelial neo-plasia ( CIN )Ⅰ group, CIN Ⅱ group, CIN Ⅲ group, and cervical cancer group were significantly higher than those in normal and inflammatory group (all P <0. 05 ). The sensitivity, correct diagnosis index, negative predictive value and positive predictive value of the combined detection improved to varying degrees and they were 92. 2% , 0.71, 99. 6% and 40. 2% , respectively. Conclusion The combined detection of high - risk HPV and TCT has important clinical significance for the early diagnosis of cervical cancer.%目的 研究高危型人乳头瘤病毒(HPV)检查及薄层液基细胞学技术(TCT)在宫颈癌与癌前病变的早期诊断的临床应用价值.方法 回顾性分析高危型HPV检查及TCT对1 248例宫颈癌筛查对象的检查结果.比较两种方法及联合应用的临床诊断价值.结果 病理组织学CINⅠ期组、CINⅡ期组、CINⅢ期组、宫颈癌组高危型HPV感染率及TCT阳性率与正常及炎症组比较均有统计学差异(P<0.05);CINⅠ-Ⅲ期及宫颈癌组高危型HPV感染率、TCT阳性率均高于正常及炎症组;高危型HPV检测与TCT两种方法联合检测灵敏度(92.2%),正确诊断指数(0.71)、阴性预测值(99.6%)及阳性预测值(40.2%)均有不同程度的提高.结论 高危型HPV检测与TCT联合对宫颈癌早期发现、早期诊断有重要的临床意义.

  2. Therapeutic Vaccination for HPV Induced Cervical Cancers

    Directory of Open Access Journals (Sweden)

    Joeli A. Brinkman

    2007-01-01

    Full Text Available Cervical Cancer is the second leading cause of cancer–related deaths in women worldwide and is associated with Human Papillomavirus (HPV infection, creating a unique opportunity to treat cervical cancer through anti-viral vaccination. Although a prophylactic vaccine may be available within a year, millions of women, already infected, will continue to suffer from HPV-related disease, emphasizing the need to develop therapeutic vaccination strategies. A majority of clinical trials examining therapeutic vaccination have shown limited efficacy due to examining patients with more advanced-stage cancer who tend to have decreased immune function. Current trends in clinical trials with therapeutic agents examine patients with pre-invasive lesions in order to prevent invasive cervical cancer. However, longer follow-up is necessary to correlate immune responses to lesion regression. Meanwhile, preclinical studies in this field include further exploration of peptide or protein vaccination, and the delivery of HPV antigens in DNA-based vaccines or in viral vectors. As long as pre-clinical studies continue to advance, the prospect of therapeutic vaccination to treat existing lesions seem good in the near future. Positive consequences of therapeutic vaccination would include less disfiguring treatment options and fewer instances of recurrent or progressive lesions leading to a reduction in cervical cancer incidence.

  3. Epidemiology and biology of cervical cancer.

    Science.gov (United States)

    Schoell, W M; Janicek, M F; Mirhashemi, R

    1999-01-01

    Worldwide, cancer of the cervix is the second leading cause of cancer death in women: each year, an estimated 500,000 cases are newly diagnosed. Among populations, there are large differences in incidence rates of invasive cervical cancer: these reflect the influence of environmental factors, screening Papanicolaou (Pap) tests, and treatment of pre-invasive lesions. The high-risk human papillomavirus (HPV) subtypes 16, 18, 31, 33, and 51 have been recovered from more than 95% of cervical cancers. We have made great strides in understanding the molecular mechanism of oncogenesis of this virus, focusing on the action of the E6 and E7 viral oncoproteins. These oncoproteins function by inactivating cell cycle regulators p53 and retinoblastoma (Rb), thus providing the initial event in progression to malignancy. Cervical cancers develop from precursor lesions, which are termed squamous intraepithelial lesions (SIL) and are graded as high or low, depending on the degree of disruption of epithelial differentiation. Viral production occurs in low-grade lesions and is restricted to basal cells. In carcinomas, viral DNA is found integrated into the host genome, but no viral production is seen. The well-defined pre-invasive stages, as well as the viral factors involved at the molecular level, make cervical carcinoma a good model for investigating immune therapeutic alternatives or adjuvants to standard treatments. PMID:10225296

  4. Programmed management of acute cervical cord trauma.

    Science.gov (United States)

    White, R J; Bryk, J P; Yashon, D; Albin, M S; Demian, Y K

    Results in ten patients admitted with the diagnosis of complete traumatic quadriplegia and with fracture-dislocation of the cervical spine are reviewed. Emphasis is placed on aggressive emergency surgical treatment of these lesions such as tracheostomy, laminectomy and cord cooling, incorporated into a detailed protocol of overall management.

  5. Telomerase in (pre)neoplastic cervical disease

    NARCIS (Netherlands)

    Wisman, GBA; De Jong, S; Meersma, GJ; Helder, MN; Hollema, H; de Vries, EGE; Keith, WN; van der Zee, AGJ

    2000-01-01

    This study was performed to determine upregulation of the human telomerase RNA component (hTR) and mRNA of the catalytic subunit of telomerase (hTERT) in (pre)malignant cervical lesions, to analyze possible intralesional heterogeneity of hTR expression, and to relate hTR and hTERT mRNA levels to tel

  6. Aberrant DNA methylation in cervical carcinogenesis

    Institute of Scientific and Technical Information of China (English)

    Hui-Juan Yang

    2013-01-01

    Persistent infection with high-risk types of human papillomavirus(HPV) is known to cause cervical cancer; however,additional genetic and epigenetic alterations are required for progression from precancerous disease to invasive cancer.DNA methylation is an early and frequent molecular alteration in cervical carcinogenesis.In this review,we summarize DNA methylation within the HPV genome and human genome and identify its clinical implications.Methylation of the HPV long control region (LCR) and L1 gene is common during cervical carcinogenesis and increases with the severity of the cervical neoplasm.The L1 gene of HPV16 and HPV18 is consistently hypermethylated in invasive cervical cancers and can potentially be used as a clinical marker of cancer progression.Moreover,promoters of tumor suppressor genes (TSGs) involved in many cellular pathways are methylated in cervical precursors and invasive cancers.Some are associated with squamous cell carcinomas,and others are associated with adenocarcinomas.Identification of methylated TSGs in Pap smear could be an adjuvant test in cervical cancer screening for triage of women with high-risk HPV,atypical squamous cells of undetermined significance,or low grade squamous intraepithelial lesion (LSIL).However,consistent panels must be validated for this approach to be translated to the clinic.Furthermore,reversion of methylated TSGs using demethylating drugs may be an alternative anticancer treatment,but demethylating drugs without toxic carcinogenic and mutagenic properties must be identified and validated.

  7. Abrasion of 6 dentifrices measured by vertical scanning interference microscopy

    Directory of Open Access Journals (Sweden)

    Florence Pascaretti-Grizon

    2013-09-01

    Full Text Available OBJECTIVES: The abrasion of dentifrices is well recognized to eliminate the dental plaque. The aims of this study were to characterize the abrasive powders of 6 dentifrices (3 toothpastes and 3 toothpowders and to measure the abrasion on a test surface by Vertical Scanning Interference microscopy (VSI. MATERIAL AND METHODS: Bright field and polarization microscopy were used to identify the abrasive particles on the crude dentifrices and after prolonged washes. Scanning electron microscopy and microanalysis characterized the shape and nature of the particles. Standardized and polished blocks of poly(methylmethacrylate were brushed with a commercial electric toothbrush with the dentifrices. VSI quantified the mean roughness (Ra and illustrated in 3D the abraded areas. RESULTS: Toothpastes induced a limited abrasion. Toothpowders induced a significantly higher roughness linked to the size of the abrasive particles. One powder (Gencix® produced a high abrasion when used with a standard testing weight. However, the powder is based on pumice particles covered by a plant homogenate that readily dissolves in water. When used in the same volume, or after dispersion in water, Ra was markedly reduced. CONCLUSION: Light and electron microscopy characterize the abrasive particles and VSI is a new tool allowing the analysis of large surface of abraded materials.

  8. Abrasion Resistant Refractory Materials GB/T 23294-2009

    Institute of Scientific and Technical Information of China (English)

    Yu Lingyuan

    2009-01-01

    @@ 1 Scope This standard specifies the definition, classification, technical requirements, test methods, quality appraisal procedure, packing, marking, transportation, storage, and quality certificate of abrasion resistant refractory materials. This standard is applicable to abrasion resistant refractory materials for circulating fluidized bed boilers, daily waste incinerator, industrial waste incinerator, medical waste incinerator, ordinary solid waste incinerator, hazardous waste incinerator, etc.

  9. Testing of Commercial Cutting Heads for Abrasive Water Jet Technology

    OpenAIRE

    Klich, J. (Jiří); Hlaváček, P.; M. Zeleňák; Sitek, L. (Libor); Foldyna, J.

    2013-01-01

    Five different cutting heads designed for cutting by high-speed abrasive water jet technology were tested from cutting ability point of view. Straight kerfs were cut in several metal materials by abrasive water jet. Material removal volume was determined as a measure of performance of specific cutting head. Quality of cutting surface was observed, too. Results are compared and discussed.

  10. Soybean seedlings tolerate abrasion from air-propelled grit

    Science.gov (United States)

    New tools for controlling weeds would be useful for soybean production in organic systems. Air-propelled abrasive grit is one such tool that performs well for in-row weed control in corn, but crop safety in soybean is unknown. We examined responses to abrasion by corn-cob grit of soybean seedlings a...

  11. 29 CFR 1910.215 - Abrasive wheel machinery.

    Science.gov (United States)

    2010-07-01

    ... 29 Labor 5 2010-07-01 2010-07-01 false Abrasive wheel machinery. 1910.215 Section 1910.215 Labor... OCCUPATIONAL SAFETY AND HEALTH STANDARDS Machinery and Machine Guarding § 1910.215 Abrasive wheel machinery. (a... wheel in motion. (5) Excluded machinery. Natural sandstone wheels and metal, wooden, cloth, or...

  12. Analysis of the Functional Independence Measure Value of Cervical Spine Injury Patients with Conservative Management

    Directory of Open Access Journals (Sweden)

    M. Zafrullah Arifin

    2012-06-01

    Full Text Available Analysis of the Functional Independence Measure Value of Cervical Spine Injury Patients with Conservative Management. Cervical spine injury is one of the most common spinal cord injuries in trauma patients. From 100,000 spinal cord injury cases reported in the United States of America (2008, sixty seven percent involve cervical spine injury. American Spinal Cord Injury Association (ASIA impairment score is used as an initial assessment but not enough attention prognostic outcome of these patients was paid to. The objective of this study is to analyze the value of functional independence measure (FIM cervical spine injury patients with conservative management and its correlation with age, sex, type of trauma, onset of trauma, cervical abnormalities, type of cervical spine lesion and ASIA impairment score. A prospective cohort study was performed to all patients with cervical spine injury treated inNeurosurgery Department of Dr. Hasan Sadikin Hospital Bandung that fullfiled the inclusion criteria. The subjects were classified based on age, sex, single/multiple trauma, acute /chronic, cervical abnormalities, complete/incomplete lesion and ASIA impairment score. The FIM examination was performed in Outpatient clinic of Neurosurgery. T-test and chi-square test was done to analyze the data. There were 17 cervical spine injury patients treated in Neurosurgery Department of Dr. Hasan Sadikin Hospital during April 2009–April 2010. The average FIM value of cervical spine injury in those patients is 4+ 1.63 by cohort prospective study. There were no correlation between FIM value with age, sex, type of trauma, onset of trauma and cervical abnormalities. Significant correlations were found between FIM value with type of cervical spine lesion and ASIA impairment score in cervical spine patients. Type of cervical spine lesion and ASIA impairment score have significant correlation with FIM value of patients in 6 months after cervical injury.

  13. Research and development of blasting abrasive made of steelmaking slag

    Institute of Scientific and Technical Information of China (English)

    TANG Oujing

    2015-01-01

    This study focuses on the development of a new type of nonmetallic steelmaking slag abrasive.The performance,processing,and application of steelmaking slag as a nonmetallic abrasive are introduced.The chemical composition,hardness,crushing value,and particle gradation of steelmaking slag are analyzed.A processing method for steelmaking slag as a blasting abrasive is suggested and evaluated.Compared with conventional abrasives such as copper ore sand and cast iron shot,processed steelmaking slag exhibits similar performance and can satisfy abrasive technical requirements.The derusting effect provided by steelmaking slag for a ship deck can reach the Sa2.0 level,and its recyclability is higher than that of copper ore sand.The derusting performance of steelmaking slag is similar to that of copper ore,and it can thus be used in repairing ship decks.

  14. 液基细胞学技术与传统细胞学涂片检测宫颈鳞状细胞病变的比较%Comparison of liquid - based cytological test and routine cytological smears in detection of cervical squamous cell lesions

    Institute of Scientific and Technical Information of China (English)

    印永祥; 赵华; 黄望珍

    2012-01-01

    目的:评价液基细胞学技术与传统细胞学涂片检测宫颈鳞状细胞病变的差异.方法:比较同地区、同期宫颈液基细胞学筛查病例及传统细胞学涂片筛查病例,同时收集液基细胞组中289例阴道镜检查及病理活检结果及传统细胞组中62例阴道镜检查及病理活检结果进行分析,评估不同病变的假阳性率.结果:液基细胞组标本不满意率(2.71%)明显低于传统细胞组标本(3.96%)(P<0.01).液基细胞组对不明意义的非典型鳞状细胞(ASC - US)及更高级别的病变检出率(2.25%)明显高于传统细胞组(0.74%)(P<0.01).液基细胞组ASC - US占低度鳞状上皮内病变(LSIL)、非典型鳞状细胞不除外高度病变( ASC - H)、高度鳞状上皮内病变(HSIL)及鳞癌(SCC)总数的比例(0.82%)明显低于传统细胞组(2.11%)(P<0.01).传统细胞组与液基细胞组假阳性率比较,差异有统计学意义(P<0.01),传统细胞组ASC -US、ASC -H和SCC中假阳性率与液基细胞组比较差异无统计学意义.组织学活检与细胞学结果比较,年龄≥35岁液基细胞组ASC - US阳性预测值与传统细胞组比较差异无统计学意义(P>0.01);年龄<35岁液基细胞组ASC - US阳性预测值明显高于传统细胞组(P<0.01).结论:液基细胞学技术检测宫颈鳞状细胞病变明显优于传统细胞学涂片,高质量的宫颈细胞标本对提高诊断的准确性非常重要;选择不同方法筛查宫颈病变时,目标人群的年龄是一个重要的决定因素.%Objective; To evaluate the difference of liquid - based cytological technique and routine cytological smears in detection of cervical squamous cell lesions. Methods; The cases receiving cervical liquid - based cytological screening and the cases receiving routine cytological smears screening in the same region and during the same period were compared. The results of colposcopy and pathological biopsy of 289 cases from liquid - based

  15. Hydro-abrasive erosion: Problems and solutions

    Science.gov (United States)

    Winkler, K.

    2014-03-01

    The number of hydro power plants with hydro-abrasive erosion is increasing worldwide. An overall approach is needed to minimize the impact of this phenomenon. Already at the start of the planning phase an evaluation should be done to quantify the erosion and the impact on the operation. For this, the influencing parameters and their impact on the erosion have to be known. The necessary information for the evaluation comprises among others the future design, the particle parameters of the water, which will pass the turbine, and the power plant owner's framework for the future operation like availability or maximum allowable efficiency loss, before an overhaul needs to be done. Based on this evaluation of the erosion, an optimised solution can then be found, by analysing all measures in relation to investments, energy production and maintenance costs as decision parameters. Often a more erosion-resistant design, instead of choosing the turbine design with the highest efficiency, will lead to higher revenue. The paper will discuss the influencing parameters on hydro-abrasive erosion and the problems to acquire this information. There are different optimisation possibilities, which will be shown in different case studies. One key aspect to reduce the erosion and prolong the operation time of the components is to coat all relevant parts. But it is very important that this decision is taken early in the design stage, as the design has to be adapted to the requirements of the coating process. The quality of coatings and their impact on the operation will be discussed in detail in the paper as due to the non-availability of standards many questions arise in projects.

  16. Lung scintigraphy evaluation in workers exposed to abrasive dusts; Avaliacao cintilografica pulmonar em trabalhadores de industria de abrasivos

    Energy Technology Data Exchange (ETDEWEB)

    Terra Filho, Mario

    1995-12-31

    The production process of abrasives use aluminium, or silicon carbide a synthetic material with a hardness only slightly less than that of a diamond. It is popularly known as carborundum since it was first manufactured as an abrasive in 1891, produced by the fusion of high grade silica and petroleum coke with sawdust. For many years silicon carbide was thought not to give rise to pulmonary lesions. Recently several researchers suggested the existence of a carborundum pneumoconiosis. The aim of this study was to evaluate the role of the pulmonary clearance of {sup 99m} Technetium chelated to diethylene-triamine penta-acetate ({sup 99m} Tc DTPA), and {sup 67} Gallium lung scanning in workers exposed to abrasive dusts. Thirty seven subjects, 13 smokers and 24 nonsmokers and ex smokers were studied. In 32 (86,48%) {sup 67} Gallium lung scanning was positive including 13 (40,62%) retired workers. We conclude that non smoking workers of abrasives plants have a pulmonary alveolar epithelial permeability disturbance similar as observed in smoking workers and smoking controls. Most workers, ex-workers of these industries and in patients with carborundum pneumoconiosis there is an evidence of pulmonary inflammation measured with abnormal {sup 67} Gallium lung scan. (author) 101 refs., 2 figs., 11 tabs.

  17. Using Intervention Mapping as a Participatory Strategy: Development of a Cervical Cancer Screening Intervention for Hispanic Women

    Science.gov (United States)

    Byrd, Theresa L.; Wilson, Katherine M.; Smith, Judith Lee; Heckert, Andrea; Orians, Carlyn E.; Vernon, Sally W.; Fernandez-Esquer, Maria E.; Fernandez, Maria E.

    2012-01-01

    Cervical cancer is preventable with treatment of precancerous lesions and treatable at early stages. Hispanics have higher rates of cervical cancer and lower rates of screening. "Ayndando a las Mujeres con Informaccion, Guia, y Amor para su Salud" (AMIGAS) is an intervention to increase cervical cancer screening in U.S. women of Mexican origin.…

  18. Diagnostic validity of cervical precancerous lesions by liquid-based cytology technique combined with high-risk HPV-DNA detection%液基细胞学技术联合高危HPV-DNA检测对宫颈癌前病变的诊断效度

    Institute of Scientific and Technical Information of China (English)

    王泽曼; 陈玉; 郑建鹏

    2011-01-01

    目的:探讨液基细胞学检查技术(LCT)联合高危人乳头状瘤病毒DNA(HPV-DNA)检测诊断宫颈癌前病变的效度.方法:对2007年12月~2010年12月来我院行体检的19~65岁的6 521名女性采用LCT进行宫颈癌的筛查,以及HPV分型基因芯片检测系统进行18种高危HPV基因亚型检测.对上述检测阳性者行阴道镜下宫颈活组织检查,对检测均阴性者依其意愿进行阴道镜下宫颈活组织检查.结果:LCT阳性(≥ASCUS)152例,HPV阳性86例,其中二者均为阳性的有42例,LCT和HPV均为阴性的有6 325例;LCT阳性的152例和HPV阳性的86例中病理活组织检查结果为阳性(≥CIN I)的分别有112例和68例,其中LCT和HPV均阳性的42例中病理活组织检查阳性的有34例.LCT和HPV均为阴性的6 325例中有2 000人自愿行病理检查,其中1人病理检查结果为阳性.LCT诊断宫颈癌前病变的灵敏度为76.19%,特异度为98.05%;HPV检测诊断宫颈癌前病变的灵敏度为46.26%,特异度为99.12%;两方法联合诊断(其中1项阳性即判定为患者)宫颈癌前病变的灵敏度为99.32%,特异度为99.61%.结论:液基细胞检测技术和高危HPV-DNA检测的联合应用优于单项技术检测,对于宫颈癌前病变的筛检具有重要意义.%AIM: To investigate liquid - based cytology technique ( LCT ) combined with high - risk human papilloma virus DNA ( HPV - DNA ) detection for diagnosis of cervical precancerous lesions. METHODS: Screening of cervical cancer was performed by LCT combined with HPV - DNA detection in 6 521 women at the age of 19 ~65 in our hospital for physical examination from December 2007 to December 2010. Eighteen high - risk HPV isoform genes were detected by HPV typing gene chip detection system. The women with positive detection underwent colposcopic cervical biopsies. The women with negative detection also underwent colposcopic cervical biopsies if the operation was of their own accord. RESULTS: The LCT positive results

  19. Research on the Clinical Value of Vaginal Ultrasound in the Diagnosis of Early Cervical Cancer and Precancerous Lesions%阴道超声在早期宫颈癌及癌前病变诊断中的临床价值研究

    Institute of Scientific and Technical Information of China (English)

    张利; 赵华巍; 郭丽娟

    2016-01-01

    目的:探讨阴道超声对早期宫颈癌及癌前病变的诊断价值。方法以43例宫颈癌患者作为研究对象,其中早期宫颈癌27例,癌前病变16例。所有患者均于术前确诊并行手术治疗。另选取体检无妇科病史的30例妇女作为对照组,对三组的阴道超声声像特征进行对比分析。结果阴道超声诊断为宫颈癌前病变者16例,准确率为81.3%;超声诊断为早期宫颈癌者25例,诊断准确率为92.6%。三组的宫颈厚径与宫颈内膜厚度比较存在显著性差异( P<0.05),三组间关系为:正常对照组<癌前病变组<早期宫颈癌组。正常对照组中均未见宫颈线中断与宫颈内出现强回声或低回声团,15例测到丰富血流;癌前病变组宫颈线中断2例,3例可见宫颈内出现强回声或低回声团,血流丰富8例;早期宫颈癌组中宫颈线中断10例,宫颈内出现强回声或低回声团8例,血流丰富22例;三组间存在显著性差异( P<0.05)。3组PSV与RI比较存在显著性差异(P<0.05);PSV:正常对照组<病前病变组<早期宫颈癌组;RI:正常对照组>病前病变组>早期宫颈癌组。结论阴道超声对早期宫颈癌及癌前病变具有较高的敏感度,可为临床提供有价值的信息,可作为细胞学检查与妇科检查的有效补充,减少漏诊或误诊病例的发生。%Objective To study the clinical value of vagianl ultrasound in the diagnosis of early cervical cancer and pre -cancerous lesions .Methods 43 cases of patients with cervical cancer were selected ,in which,27 cases were early cervical cancer and 16 cases were precancerous lesions .All patients were diagnosed before operation .30 cases of women without history of gynae-cology and symptoms were selected as the control group .The characteristics of vaginal ultrasound acoustic images were compared and analyzed.Results Among these cases,16 cases were

  20. Mechanics, kinematics and geometry of pebble abrasion from binary collisions

    Science.gov (United States)

    Miller, K. L.; Jerolmack, D. J.

    2014-12-01

    As sediment is transported downstream as bedload, it collides with the bed causing sharp edges to chip and wear away, rounding the rock through the process of abrasion. Previous work has linked abrasion to downstream fining and rounding of grains, however, there has been little attempt to understand the underlying kinematics of abrasion. Furthermore, most studies neglect the fine particle produced during the abrasion process, as the initial grain gets smaller and rounder. In this research, we preform well-controlled laboratory experiments to determine the functional dependence between impact energy and mass lost from abrasion. We use a double-pendulum "Newton's Cradle" set-up to examine the abrasion between two grains and with a high-speed camera, we can quantify the impact energies during collision. Results from experiments verify that mass loss is proportional to kinetic energy. We define a material parameter that incorporates material density, Young's modulus, and tensile stress and show that this parameter is directly related to the proportionality between mass loss and energy. We identify an initial region of the mass loss curves in which abrasion is independent of energy and material properties; results suggest this region is determined by shape. We show that grain size distributions of daughter products are universal and independent of material; they follow a Weibull distribution, which is expected distribution from brittle fracture theory. Finally, scanning electron microscope (SEM) images show a thin damage zone near the surface, suggesting that collision energy is attenuated over some small skin depth. Overall, we find that pebble abrasion by collision can be characterized by two universal scaling relations - the mass loss versus energy curves and the size distribution of daughter products. Results will be useful for estimating expected abrasion rates in the field, and additionally demonstrate that low-energy collisions produce large quantities of sand

  1. Some results of tangential turning with an abrasive water jet

    OpenAIRE

    Cárach, J.; Hlaváček, P.; K. Vasilko; Klich, J. (Jiří); S. Hloch

    2013-01-01

    This article deals with the visual comparison of the finished surface of sandstone using the tangential abrasive water jet. The continual abrasive water jet with turning was used with the constant pressure of 400 MPa for all sections of turning and with a depth of cut of 2mm. The abrasive particles were added to the water jet in the amount of 400 g.min-1. The traverse speed (vf = 60, 30, 10 mm.min-1) was gradually changing at a constant speed of rotation of workpiece n = 30min-1. Th...

  2. An Investigation of Different Material on Abrasive Water jet Machine

    OpenAIRE

    Vaibhav.j.limbachiya; Prof Dhaval.M.Patel

    2011-01-01

    Abrasive water jet machine (AWJM) is a nontraditional machining process. Abrasive water jet machining is a process of removal of material by impact erosion of high pressure (1500-4000 bar), high velocity of water and entrained high velocity of grit abrasives on a work piece. It’s a non-conventional machining process. At herefirst works on theoretical work after it make some experimental work and then analyses both results. Theoretical MRR found equal to the experimental MRR. In this paper inv...

  3. 高危型 HPV 结合液基细胞学技术在宫颈癌前病变诊断中的应用%Application of High-risk HPV Combined with Liquid-based Cytology Technology in the Diagnosis of Cervical Lesions

    Institute of Scientific and Technical Information of China (English)

    颜萍; 龚旭华

    2014-01-01

    Objective To investigate the application of high-risk HPV genotyping assay combined with liquid-based cy-tology for cervical lesions .Methods Liquid-based cytology samples of 204 cases of cervical cancer were detected by high-risk HPV genotyping assay ,and were compared with cervical biopsy results .Results 138 patients were TCT-positive combined with HPV infection,LSIL showed that HPV-positive was 40 cases (29.0%),HSIL showed that HPV-positive was 10 cases (7.2%), ASCUS showed that HPV-positive was 80 cases(58.0%),SCC showed that HPV-positive was 8 cases (5.8%).High-risk HPV was 42 cases,and positive rate was 30.4%.Samples of different types of high-risk HPV infection had significant difference ( P<0.05).When TCT and HPV were both ( -),there had no high grade lesion .When HPV-positive and TCT abnormal both oc-curred,detection rate of ≥CINⅠwas 34.8%.When TCT was abnormal HPV (-),the detection rate was 47.8%.Conclusion High-risk HPV genotyping assay combined with liquid-based cervical cytology is a valuable method for early screening of cervical cancer,the result is more reliable than single inspection .%目的:探讨高危型HPV基因分型检测结合液基细胞学检查在宫颈癌前病变诊断中的应用。方法纳入204例宫颈癌患者,采集液基细胞标本后,进行高危型HPV基因分型检测,并与宫颈活检结果进行比较分析。结果138例患者显示TCT阳性合并HPV感染,其中LSIL显示HPV阳性40例(29.0%),HSIL显示HPV阳性10例(7.2%),AS-CUS显示HPV阳性80例(58.0%),SCC显示HPV阳性8例(5.8%)。42例HPV高危型,阳性率为30.4%。不同类型标本HPV高危型感染率具有显著性差异( P<0.05)。 TCT和HPV均为(-)时,则未发生高度病变。 HPV阳性和TCT异常同时存在时,≥CINⅠ的检出率为34.8%。仅TCT异常而HPV(-)时,检出率为47.8%。结论高危型HPV基因分型检测结合液基细胞学检查是极具价

  4. Effect of abrasive grit size on wear of manganese-zinc ferrite under three-body abrasion

    Science.gov (United States)

    Miyoshi, Kazuhisa

    1987-01-01

    Wear experiments were conducted using replication electron microscopy and reflection electron diffraction to study abrasion and deformed layers produced in single-crystal Mn-Zn ferrites under three-body abrasion. The abrasion mechanism of Mn-Zn ferrite changes drastically with the size of abrasive grits. With 15-micron (1000-mesh) SiC grits, abrasion of Mn-Zn ferrite is due principally to brittle fracture; while with 4- and 2-micron (4000- and 6000-mesh) SiC grits, abrasion is due to plastic deformation and fracture. Both microcracking and plastic flow produce polycrystalline states on the wear surfaces of single-crystal Mn-Zn ferrites. Coefficient of wear, total thickness of the deformed layers, and surface roughness of the wear surfaces increase markedly with an increase in abrasive grit size. The total thicknesses of the deformed layers are 3 microns for the ferrite abraded by 15-micron SiC, 0.9 microns for the ferrite abraded by 4-micron SiC, and 0.8 microns for the ferrite abraded by 1-micron SiC.

  5. New Rock Abrasivity Test Method for Tool Life Assessments on Hard Rock Tunnel Boring: The Rolling Indentation Abrasion Test (RIAT)

    Science.gov (United States)

    Macias, F. J.; Dahl, F.; Bruland, A.

    2016-05-01

    The tunnel boring machine (TBM) method has become widely used and is currently an important presence within the tunnelling industry. Large investments and high geological risk are involved using TBMs, and disc cutter consumption has a great influence on performance and cost, especially in hard rock conditions. Furthermore, reliable cutter life assessments facilitate the control of risk as well as avoiding delays and budget overruns. Since abrasive wear is the most common process affecting cutter consumption, good laboratory tests for rock abrasivity assessments are needed. A new abrasivity test method by rolling disc named Rolling Indentation Abrasion Test (RIAT) has been developed. The goal of the new test design and procedure is to reproduce wear behaviour on hard rock tunnel boring in a more realistic way than the traditionally used methods. Wear by rolling contact on intact rock samples is introduced and several rock types, covering a wide rock abrasiveness range, have been tested by RIAT. The RIAT procedure indicates a great ability of the testing method to assess abrasive wear on rolling discs. In addition and to evaluate the newly developed RIAT test method, a comprehensive laboratory testing programme including the most commonly used abrasivity test methods and the mineral composition were carried out. Relationships between the achieved results from conventional testing and RIAT results have been analysed.

  6. 人乳头瘤病毒、端粒酶基因及3号染色体数目与宫颈病变关系的探讨%Study on relationship between human papilloma virus,telomerase gene and chromosome 3 number with cervical lesions

    Institute of Scientific and Technical Information of China (English)

    李亚波; 杨隽; 丁世霖; 杨红英; 王卓

    2016-01-01

    Objective To explore the relationship between the expression of human telomerase RNA component (TERC) gene , human papilloma virus (HPV) infection and mutation of chromosome 3 number with cervical lesions .Methods 81 women received the treatment in the Gynecology Department of the Second Affiliated Hospital of Kunming Medical University from June 2008 to February 2009 ,including the healthy group(normal pathological examination ,20 cases) ,CIN1 group(28 cases) ,CIN2 group(12 ca‐ses) ,CIN3 group(9 cases) and cervical cancer group(12 cases) .The TERC gene expression in uterine epithelial exfoliated cells was detected by using the fluorescence in situ hybridization (FISH) method ,meanwhile the HPV infection was detected by using the real time fluorescence quantitative polymerase chain reaction (FQPCR) technology .The correlation between cervical cancer with TERC gene and HPV was analyzed .At the same time the number of chromosome 3 mutations in 81 cases was recorded .Results In the cervical lesion detection ,the detection positive rate had no statistical difference between the TERC gene detection and HPV detec ‐tion (P> 0 .05) ,their positive rates in the CIN 1 ,CIN2 ,CIN3 and cervical cancer groups were significantly higher than that in the healthy group (P 0 .05) , while between the CIN3 group and the cervical cancer group had statistical significance (P< 0 .05) ,the higher the malignant degree , the higher the positive rate .The abnormal mutation rate of chromosome 3 number was 0% in the healthy group and the CIN1 group ,16 .7% in the CIN2 group ,66 .7% in the CIN3 group and 100 .0% in the cervical cancer group ,the positive rate in the CIN3 group and the cervical cancer group was significantly higher than that in the healthy group ,CIN1 group and CIN2 group ,the differ‐ences were statistically significant (P< 0 .05) .Conclusion The TERC abnormal gene expression ,high risk HPV infection and mutation of chromosome 3 number could play an important synergistic

  7. Abrasion Properties of Steel Fiber Reinforced Silica Fume Concrete According to Los Angeles and Water Abrasion Tests

    Directory of Open Access Journals (Sweden)

    Tsan-Ching CHENG

    2014-12-01

    Full Text Available The current study mainly investigated the influence of different tests on the abrasion resistance of concrete mixed with steel fibers and silica fume. The abrasion resistance was assessed at 28, 56 and 91 days on concretes with water-binder ratios of 0.35 and 0.55 where in some mixes silica fume was substituted by 5 % of cement by weight. Steel fibers of 0.5 % and 1.0 % of concrete volume were also added into the test concrete by replacement of coarse and fine aggregates. The results showed that concrete with higher compressive strength in Los Angeles abrasion tests also had better abrasion resistance. The inclusion of steel fibers into test concrete with a water-binder ratio of 0.35 resulted in a significant increase in compressive strength. This concrete also displayed better abrasion resistance and splitting tensile strength than reference concrete; in the test sample with a water-binder ratio of 0.55, the added steel fibers was unable to effectively produce cementation with the concrete. The inclusion of silica fume improved the abrasion resistance of concretes. In water abrasion testing, the abrasion resistance of concrete containing steel fiber was worse than that of concrete without steel fibers. In the water abrasion testing, the surface of steel fiber reinforced concrete was eroded by water and steel balls, and the impact caused the steel fibers to separate from the concrete and led to higher wear loss. DOI: http://dx.doi.org/10.5755/j01.ms.20.4.6460

  8. Relationship between Severity of Cervical Lesions and Vaginal Environment in Patients with High-Risk HPV Infection%高危型人乳头瘤病毒感染者宫颈病变程度与阴道内环境的关系

    Institute of Scientific and Technical Information of China (English)

    李岩

    2015-01-01

    目的:探讨高危型人乳头瘤病毒(HPV)感染者宫颈病变严重程度与阴道内环境的关系。方法选取425例高危型 HPV 感染患者,均行宫颈液基薄层细胞学检查(TCT)以了解宫颈病变程度,并行阴道内环境监测,对比不同程度宫颈病变者阴道内环境指标的差异。结果TCT 检查:正常13例,炎症132例,CINⅠ级111例,CINⅡ级90例,CIN Ⅲ级54例,宫颈癌25例。阴道内环境检测:阴道清洁度≤Ⅱ级者194例,pH 值≤4.5者202例,菌群分布≤Ⅰ级者244例,滴虫感染者56例,假丝酵母菌感染者48例,阴道炎患者240例。不同程度宫颈病变阴道内环境指标对比:阴道清洁度≥Ⅲ级、pH 值≤4.5、菌群分布≥Ⅱ级、阴道炎的发生率在不同 TCT 检查结果的患者中差异有统计学意义(均 P <0.05),而滴虫感染及假丝酵母菌感染发生率在不同 TCT 检查结果的患者中较差异无统计学意义(P >0.05)。结论阴道内环境的改变与 HPV 感染存在密切关系,阴道内环境动态平衡的破坏可能对 HPV 感染者宫颈病变的进一步发展起到了一定促进作用,但两者的确切关系有待进一步研究。%ABSTRACT:Objective To investigate the relationship between the severity of cervical lesions and vaginal environment in patients with high-risk human papillomavirus(HPV)infection.Meth-ods The severity of cervical lesions was detected by thin-layer cytological test(TCT)and vaginal environment was monitored in 425 patients with HPV infection.The indicators of vaginal environ-ment were compared among patients with different degrees of cervical lesions.Results Among the 425 patients,TCT showed normal in 13,inflammation in 132,CIN Ⅰ in 111,CIN Ⅱ in 90, CIN Ⅲ in 54,and cervical cancer in 25.Vaginal environment monitoring showed vaginal cleanli-ness≤Ⅱ in 194,pH≤4.5 in 202,bacterial flora distribution≤Ⅰ in 244,Trichomonas infection in

  9. Marijuana Use is Not Associated with Cervical Human Papillomavirus Natural History or Cervical Neoplasia in HIV-seropositive or HIV-seronegative Women

    OpenAIRE

    D’Souza, G; Palefsky, J.M.; Zhong, Y; Minkoff, H; Massad, L.S.; Anastos, K; Levine, AM; Moxley, M; Xue, X.; Burk, R; Strickler, HD

    2010-01-01

    Marijuana use was recently reported to have a positive cross-sectional association with human papillomavirus (HPV)-related head and neck cancer. Laboratory data suggest that marijuana could have an immunomodulatory effect. Little is known, however, regarding the effects of marijuana use on cervical HPV or neoplasia. Therefore, we studied the natural history (ie, prevalence, incident detection, clearance/persistence) of cervical HPV and cervical neoplasia (ie, squamous intraepithelial lesions ...

  10. HPV阴性的宫颈癌和癌前病变中p14ARF蛋白表达及其临床意义%Expression and clinical significance of p14ARF protein in HPV - negative patients with cervical cancer and cervical precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    尹香花; 苏悦; 隋玉梅; 成艳

    2011-01-01

    Objective:To explore the relationship between pl4ARF protein, matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs) and early diagnosis, invasion and metastasis of cervical cancer by detecting their expression levels in cervical canc-er and cervical intraepithelial neoplasia (CIN) of human papillomavirus ( HPV ) - negative patients. Methods: 30 HPV - negative patients with chronic cervicitis, 35 HPV - negative patients with CIN and 35 HPV - negative patients with cervical invasive cancer were selected as study ob-jects by PCR. Immunohistochemical SP staining was used to detect the expression level of pl4ARF protein, clinicopathological characteristics and the correlation with the depth of invasion and lymph node metastasis. Results :P14ARF protein specifically expressed in the nuclei and cyto-plasm of CIN and cervical cancer cells, and it never expressed in normal squamous epithelium and glandular epithelium, there was no correla-tion between P14ARF protein expression and clinical stages, while it was related to the degree of differentiation and lymph node metastasis. Conclusion: pl4ARF protein detection can be used as an index for early diagnosis of cervical cancer among HPV - negative patients, which also can be used as an index to predict the potential of invasion and metastasis of cervical cancer and clinical prognosis.%目的:通过检测人乳头状瘤病毒(HPV)感染阴性的官颈癌和宫颈上皮内瘤变中p14ARF和基质金属蛋白酶MMPs及其抑制物TIMPs的表达,探讨其与官颈癌早期诊断、侵袭转移的关系.方法:以PCR方法筛查HPV阴性的慢性官颈炎30例、官颈上皮内瘤变(CIN) 35例及官颈浸润癌35例作为研究对象,采用SP染色法检测p14ARF蛋白在其中的表达,分析p14ARF蛋白在官颈癌组织中的表达、临床病理特征及其与浸润深度、淋巴结转移的相关性.结果:p14ARF蛋白特异性表达在HPV阴性的CIN病变、宫颈癌细胞核及胞质中,在

  11. An Investigation of Different Material on Abrasive Water jet Machine

    Directory of Open Access Journals (Sweden)

    Vaibhav.j.limbachiya

    2011-07-01

    Full Text Available Abrasive water jet machine (AWJM is a nontraditional machining process. Abrasive water jet machining is a process of removal of material by impact erosion of high pressure (1500-4000 bar, high velocity of water and entrained high velocity of grit abrasives on a work piece. It’s a non-conventional machining process. At herefirst works on theoretical work after it make some experimental work and then analyses both results. Theoretical MRR found equal to the experimental MRR. In this paper investigation for three different materials like en8,acrylic and aluminum is carried out using Taguchi design of experiment method. Experiments are carried out using L25 Orthogonal array by varying Material traverse speed and abrasive mass flow rate for each material respectively. Anova carried out for identifies significant parameters.

  12. Refractory, Abrasive, and Other Industrial Minerals Operations - Direct Download

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — This map layer includes refractory, abrasive, and other industrial minerals operations in the United States. The data represent commodities covered by the Minerals...

  13. Anti-abrasive nanocoatings current and future applications

    CERN Document Server

    2015-01-01

    This book provides an overview of the fabrication methods for anti-abrasive nanocoatings. The connections among fabrication parameters, the characteristics of nanocoatings and the resulting properties (i.e. nanohardness, toughness, wear rate, load-bearing ability, friction coefficient, and scratch resistance) are discussed. Size-affected mechanical properties of nanocoatings are examined, including their uses. Anti-abrasive nanocoatings, including metallic-, ceramic-, and polymeric-based layers, as well as different kinds of nanostructures, such as multi-layered nanocomposites and thin films, are reviewed. * Provides a comprehensive overview of the fabrication methods for anti-abrasive nanocoatings* Discusses the connections among fabrication parameters, the characteristics of nanocoatings and the resulting properties* Reviews advantages and drawbacks of fabrication methods for anti-abrasive nanocoatings and clarifies the place of these nanocoatings in the world of nanotechnology

  14. Computed tomography to quantify tooth abrasion

    Science.gov (United States)

    Kofmehl, Lukas; Schulz, Georg; Deyhle, Hans; Filippi, Andreas; Hotz, Gerhard; Berndt-Dagassan, Dorothea; Kramis, Simon; Beckmann, Felix; Müller, Bert

    2010-09-01

    Cone-beam computed tomography, also termed digital volume tomography, has become a standard technique in dentistry, allowing for fast 3D jaw imaging including denture at moderate spatial resolution. More detailed X-ray images of restricted volumes for post-mortem studies in dental anthropology are obtained by means of micro computed tomography. The present study evaluates the impact of the pipe smoking wear on teeth morphology comparing the abraded tooth with its contra-lateral counterpart. A set of 60 teeth, loose or anchored in the jaw, from 12 dentitions have been analyzed. After the two contra-lateral teeth were scanned, one dataset has been mirrored before the two datasets were registered using affine and rigid registration algorithms. Rigid registration provides three translational and three rotational parameters to maximize the overlap of two rigid bodies. For the affine registration, three scaling factors are incorporated. Within the present investigation, affine and rigid registrations yield comparable values. The restriction to the six parameters of the rigid registration is not a limitation. The differences in size and shape between the tooth and its contra-lateral counterpart generally exhibit only a few percent in the non-abraded volume, validating that the contralateral tooth is a reasonable approximation to quantify, for example, the volume loss as the result of long-term clay pipe smoking. Therefore, this approach allows quantifying the impact of the pipe abrasion on the internal tooth morphology including root canal, dentin, and enamel volumes.

  15. [Dependence of the dentinal abrasion of human teeth on their microhardness].

    Science.gov (United States)

    Remizov, S M; Pruzhanskiĭ, L Iu

    1989-01-01

    The dentin resistance against abrasion was studied as related to its microhardness. Sections of 15 intact teeth were investigated (central upper incisors). Water suspensions (40% weight-to weight) of dicalcium phosphate (DCP, FRG; and DKF-1 and DKF-2, USSR) were used as abrasives. Dentin microhardness was measured with a PMT-3 device, and abrasion assessed with profilographic technique. Dentin abrasion was related to its microhardness and to the kind of abrasive used. Dentin abrasion increased as its microhardness decreased. DCF showed minimal abrasive effect, DKF-2 had maximal effect with DKF-1 keeping the intermediate position.

  16. Hardness and elasticity of abrasive particles measured by instrumented indentation

    OpenAIRE

    Hvizdoš, P.

    2015-01-01

    Basic mechanical properties of seven types (from seven different sites) of abrasive garnet particles used for water jet cutting were measured using the technique of instrumented indentation (also called depth sensing indentation or nanoindentation). Hardness and modulus of elasticity were evaluated and compared. All the abrasives had similar measured mechanical properties (hardness 20 – 24.16 GPa), the highest values were found for the Czech garnet.

  17. A review of micro-scale abrasion testing

    International Nuclear Information System (INIS)

    Micro-scale abrasion (commonly referred to as 'ball cratering') is a small-scale tribological test method which can be operated on a desktop. It offers the possibility of providing a quick, cheap, localized abrasion test that can be used with small samples. In principle its operation is simple, but in practice there are issues with wear scar measurement, wear mode and its applicability to a wide variety of monolithic materials and coatings. (topical review)

  18. High-risk HPV presence in cervical specimens after a large loop excision of the cervical transformation zone: significance of newly detected hr-HPV genotypes.

    NARCIS (Netherlands)

    Ham, M.A. van; Hamont, D. van; Bekkers, R.L.M.; Bulten, J.; Melchers, W.J.G.; Massuger, L.F.A.G.

    2007-01-01

    Large loop excision of the cervical transformation zone (LLETZ) is a well-established treatment for high-grade cervical intraepithelial neoplasia. It has even been postulated that LLETZ is responsible for the elimination of the infectious agent, human papillomavirus (HPV), causing the lesion. Most s

  19. Cervical Cancer Screening

    Science.gov (United States)

    ... Cancer found early may be easier to treat. Cervical cancer screening is usually part of a woman's health ... may do more tests, such as a biopsy. Cervical cancer screening has risks. The results can sometimes be ...

  20. CLINICOPATHOLOGICAL STUDY OF CERVICAL LYMPHADENOPATHY

    Directory of Open Access Journals (Sweden)

    Pradeep Kulal

    2015-07-01

    Full Text Available BACKGROUND AND OBJECTIVE : The analysis of lymph node enlargement in the neck is not a n easy task. It is challenge for surgeon to assess its clinical behaviour and come to a final diagnosis. These diseases which can be neoplastic also demands correct diagnosis for further management. The study intends to find out systematically the various p athological conditions presenting with enlarged lymph nodes in the neck, also various modes of clinical presentation and behaviour of these conditions. Relevant investigations have also been studied. METHODS AND MATERIALS : The study population consisted of patients above 12 years presenting with cervical lymph node enlargement. The material consists of patients during the period of January 2011 to J uly 2012. This study consists of 100 consecutive cases. Diagnosis is made on the basis of histopathological fi ndings. Patient was examined systemically giving utmost importance to local examination. After making a clinical diagnosis, further relevant investigations were done to confirm the diagnosis. Treatment was instituted appropriately and followed up the patie nts. RESULTS : Majority of the cases in this study had non - neoplastic causes for cervical lymphadenopathy in which tuberculosis is most common. Male and female ratio of 1.38:1 is noted with most cases between 12 and 30 years. Posterior triangle group of lym ph nodes was most commonly affected in tuberculosis. In lymphomas level 2 group of among the groups of lesions, with regard to local characteristics like number, laterality , mobility and involvement of other group of lymph nodes etc .FNAC by virtue of it being inexpensive, quick in getting results and easy to perform , is one of the important and essential diagnostic procedures. INTERPRETATION AND CONCLUSION : Clinical symptoms in cervical lymphadenopathy is of limited significance because clinical behaviour can be highly variable As cervical lymphadenopathy is an important disease, it always

  1. Cervical Cancer Stage IA

    Science.gov (United States)

    ... historical Searches are case-insensitive Cervical Cancer Stage IA Add to My Pictures View /Download : Small: 720x576 ... Large: 3000x2400 View Download Title: Cervical Cancer Stage IA Description: Stage IA1 and IA2 cervical cancer; drawing ...

  2. 宫颈病变组织中人类染色体端粒酶基因表达与人乳头瘤病毒亚型感染相关分析%Correlation between expression of human telomerase RNA component and infection of human papillomavirus in cervical lesion

    Institute of Scientific and Technical Information of China (English)

    韩英; 李敏; 段玲

    2012-01-01

    positive expression rate of hTERC was 7. 31%, 34. 48% , 62. 86% and 75. 00% in CIN I , CIN Ⅱ, CIN Ⅲ and SCC group, which showed significant differences compared with control group (P<0.05). The positive rate of hTERC was significantly higher in CIN HI and SCC group than that in CIN I group (P<0.05). The positive expression rate of hTERC was positively correlated with the severity of cervical disease (r=0.931, P=0.031). The positive rate of HPV DNA was 15. 00%, 45. 71% and 87. 50% respectively in control group, CIN I + Ⅱ + Ⅲ group and SCC group, which showed significant differences (P<0.05). The expression of hTERC was positively correlated with high-risk HPV infection (r=0.381, P = 0.002). Conclusion The hTERC amplification occurs in more patients with severe cervical lesions and cervical cancer, which is correlated with high-risk HPV infection and may be a predictable index of the progression of cervical cancer.

  3. 阴道镜检查及活检联合高频电波刀治疗宫颈病变的临床疗效%THE CLINICAL CURATIVE EFFECT OF VAGINAL ENDOSCOPY AND BIOPSY JOINTED WITH HIGH-FREQUENCY WAVES KNIFE TREATMENT ON CERVICAL LESIONS

    Institute of Scientific and Technical Information of China (English)

    张玲娟

    2011-01-01

    [Objective] Combined with clinical practice experience, to explore the diagnosis and treatment of cervical intraepithelial neoplasia. [Methods] From January 2007-December 2009, 173 CIN patients' clinical data in our hospital were retrospectively analyzed. [Results] 173 patients with CIN, the detection rates with colposcopy and cytology diagnosis were higher than cervical pap smear classification method, the difference was significant (P< 0.05). Colposcopy was significantly better than liquid-based cytology method, the difference was significant (P < 0.05). The operative time, blood loss, repair time and cost in LEEP conization group were less than the traditional cone biopsy group, the difference was statistically significant (P < 0.05). The sustained lesions cases, the number of cervical stenosis and relapse cases in two groups were 0. [Conclusion] Colposcopy is an effective way to diagnose CIN. Colposcopy and biopsy combined frequency radiosurgical knife treatment for cervical lesions has a shorter operation time, less bleeding, high cure rate, retention of reproductive function, and does not affect pregnancy after the operation, acceptation by paticr be is worthy of promotion.%[目的]结合临床实践经验,探讨宫颈上皮内瘤样病变的诊断及治疗方法.[方法]2007年1月~2009年12月对某院收治的173例CIN的临床资料进行回顾性分析.[结果]173例CIN患者中,阴道镜检查与液基细胞学检测法诊断符合率均明显高于宫颈涂片巴氏分级法,差异有统计学意义(P<0.05).并且阴道镜检查诊断符合率明显优干液基细胞学检测法,差异有统计学意义(P<0.05).LEEP锥切组的手术时间、术中出血量、宫颈修复时间以及手术费用均少于传统锥切组,差异有统计学意义(P<0.05),且两组患者的病变持续例数、术后颈管狭窄例数和复发例均为0.[结论]阴道镜检查是诊断CIN的有效方法,阴道镜检查及活检联合高频电波刀治疗宫

  4. A CAD System for Lesion Detection in Cervigram Based on Laws Textural Feature

    Directory of Open Access Journals (Sweden)

    RamaPraba P.S

    2014-01-01

    Full Text Available Cervical cancer is the second most common cancer among the women worldwide. A computer aided diagnosis system can help colposcopist to analyze cervical images more accurately. This work aims to detect lesion in cervical images based on Laws textural feature and nearest neighbor classifier and it can be used as a diagnostic tool. The images used for the detection of cervical cancer are taken by using colposcope which magnifies the cells of cervix. The Laws textural features are extracted from the cervical images and input to nearest neighbor classifier. A totally 240 images are used for the evaluation and an overall accuracy of 96% is obtained.

  5. Differences in human papillomavirus type distribution in high-grade cervical intraepithelial neoplasia and invasive cervical cancer in Europe

    DEFF Research Database (Denmark)

    Tjalma, Wiebren A; Fiander, Alison; Reich, Olaf;

    2013-01-01

    Knowledge of differences in human papillomavirus (HPV)-type prevalence between high-grade cervical intraepithelial neoplasia (HG-CIN) and invasive cervical cancer (ICC) is crucial for understanding the natural history of HPV-infected cervical lesions and the potential impact of HPV vaccination...... on cervical cancer prevention. More than 6,000 women diagnosed with HG-CIN or ICC from 17 European countries were enrolled in two parallel cross-sectional studies (108288/108290). Centralised histopathology review and standardised HPV-DNA typing were applied to formalin-fixed paraffin-embedded cervical...... specimens dated 2001-2008. The pooled prevalence of individual HPV types was estimated using meta-analytic methods. A total of 3,103 women were diagnosed with HG-CIN and a total of 3,162 with ICC (median ages: 34 and 49 years, respectively), of which 98.5 and 91.8% were HPV-positive, respectively. The most...

  6. New Molecular Tools for Efficient Screening of Cervical Cancer

    Directory of Open Access Journals (Sweden)

    Magnus von Knebel Doeberitz

    2001-01-01

    Full Text Available Cytological screening using the Pap-smear led to a remarkable reduction of the mortality of cervical cancer. However, due to subjective test criteria it is hampered by poor inter- and intra-observer agreement. More reproducible assays are expected to improve the current screening and avoid unnecessary medical intervention and psychological distress for the affected women. Cervical cancer arises as consequence of persistent high risk papillomavirus (HR-HPV infections. Expression of two viral oncogenes, E6 and E7, in epithelial stem cells is required to initiate and maintain cervical carcinogenesis and results in significant overexpression of the cellular p16INK4a protein. Since this protein is not expressed in normal cervical squamous epithelia, screening for p16INK4a over-expressing cells allows to specifically identify dysplastic lesions, and significantly reduces the inter-observer disagreement of the conventional cytological or histological tests. Progression of preneoplastic lesions to invasive cancers is associated with extensive recombination of viral and cellular genomes which can be monitored by detection of papillomavirus oncogene transcripts (APOT assay derived from integrated viral genome copies. Detection of integrated type oncogene transcripts points to far advanced dysplasia or invasive cancers and thus represents a progression marker for cervical lesions. These new assays discussed here will help to improve current limitations in cervical cancer screening, diagnosis, and therapy control.

  7. 健康妇女及宫颈上皮癌瘤患者HPV感染基因型分布特征研究%A comparative study of HPV infective genotypes distribution in cells of normal cervix and tissues of cervical lesions

    Institute of Scientific and Technical Information of China (English)

    龙秀荣; 王志蕙; 耿建祥; 朴正爱; 肖蔚; 万美珍; 王旭波; 兰建云; 张劲松

    2012-01-01

    目的 探讨南京地区一般女性人群宫颈细胞、宫颈上皮内瘤变及宫颈癌组织中23种人乳头瘤病毒感染基因型别的分布情况及其临床意义.方法 采用基因扩增结合基因芯片技术对1 000例一般女性人群宫颈细胞、108例宫颈上皮内瘤变及62例宫颈癌组织标本进行23种HPV基因型别的检测,并对受检者进行相关资料分析.结果 1 000例一般女性人群检出HPV感染者106例,总的HPV感染率为10.60%(106/1 000);108例宫颈上皮内瘤变患者检出HPV感染者99例,总的HPV感染率为91.67%(99/108);62例宫颈癌患者检出HPV感染者52例,总的HPV感染率为83.87%(52/62).结论 基因扩增结合基因芯片检测技术可应用于宫颈细胞和组织标本,一次可检测23种HPV基因型别,特异性强,敏感性高,对中国女性宫颈HPV感染分子流行病学的调查、宫颈癌和癌前病变的防治及其疫苗的研究具有重要的意义.%Objective To compare the clinical distribution of 23 kinds of human papillomavirus genotypes in cells of normal cervix and tissues of cervical lesions and its clinical significance. Methods Polymerase chain reaction and gene-chips technology were utilized for the detection of 23 kinds of HPV genotypes in cells from 1000 cases of normal cervix 、 tissue specimens from 108 cases of cervical intraepithelial neoplasia(CIN) and from 62 cases of cervical cancers. And related materials of all subjects were analyzed. Results In 1 000 cases of normal cervix,total infection rate of HPV was 10. 60% (106/1000) ;In 108 cases of CIN,total infection rate of HPV was 91. 67%(99/108) ;In 62 cases of cervical cancers, total infection rate of HPV was 83. 87% (52/62). Conclusion Gene chip technology can detect single and multiple HPV genotypes in cervical cell and tissue samples with high sensitivity and specificity. Detection of HPV genotypes could be used to understand the prevalence situation of HPV infection and further to supply reference

  8. Detection of high-risk human papilloma virus and its clinical value-a study in women with cervical lesion in Wenzhou, Zhejiang%浙江省温州市宫颈病变妇女中高危型人乳头状瘤病毒检测及其临床价值

    Institute of Scientific and Technical Information of China (English)

    张玉宇; 蔡平生

    2013-01-01

    目的 分析宫颈病变患者中高危型人乳头状瘤病毒(high-risk human papillomavirus,HR-HPV)感染情况及特点,探讨HR-HPV DNA用于检测高级别宫颈上皮内瘤变(CIN)价值.方法 回顾分析采用液基薄层细胞学(TCT)检查、HR-HPV检测第二代杂交捕获技术(HC-Ⅱ法)和阴道镜检查并行活检的1130例宫颈病变患者临床资料.结果 1130例患者HR-HPV阳性率65.84% (744/1130).细胞学检测异常862例,不典型鳞状细胞(ASCUS)356例、不能除外高度鳞状上皮内病变的不典型鳞状细胞(ASCH) 84例、低度鳞状上皮内病变(LSIL) 216例、高度鳞状上皮内损伤(HSIL) 184例、癌22例.病理学诊断≥CIN Ⅰ/HPV Ⅰ 682例,HR-HPV阳性率78.59%(536/682).≥CINⅡ病变TCT方法灵敏度88.94%,特异度32.73%;HC-Ⅱ方法灵敏度90.21%,特异度51.82%;两种方法联合灵敏度97.45%,特异度22.42%,阳性预测值47.22%,阴性预测值92.50%.30 ~ 39岁患者除与 40 ~ 49岁有相近HR-HPV发生率外(x2=0.41,P>0.05),比20 ~ 29岁和50 ~ 59岁发病率高(x2=3.99和8.15,P <0.05,>60岁除外).HC-Ⅱ方法检测ASCUS病理≥CINⅡ灵敏度89.83%,特异性53.78%;HC-Ⅱ检测LSIL≥CINⅡ病变的灵敏度97.62%,特异性22.72%.结论 HR-HPV在各年龄段均有较高感染率,随着宫颈病变程度加深,HR-HPV感染率逐步升高.HC-Ⅱ检测HR-HPV DNA是筛查宫颈上皮内瘤变可选用方法,特别是对高级别CIN有较高的灵敏度、特异度、阳性预测值和阴性预测值.HR-HPV DNA检测是一种有效的ASCUS和LSIL的管理手段,有较高的灵敏度和阴性预测值.%Objective To understand the current status of high-risk human papilioma virus (HR-HPV) infection in women with cervical lesion,and evaluate the significance of HR-HPV detection by hybrid capture Ⅱ (HC-Ⅱ) in screening and diagnosing cervical lesion,especially high grade cervical intraepithelial neoplasia (CIN).Methods The retrospective analysis was

  9. Fractal Analysis of Cervical Intraepithelial Neoplasia

    Science.gov (United States)

    Fabrizii, Markus; Moinfar, Farid; Jelinek, Herbert F.; Karperien, Audrey; Ahammer, Helmut

    2014-01-01

    Introduction Cervical intraepithelial neoplasias (CIN) represent precursor lesions of cervical cancer. These neoplastic lesions are traditionally subdivided into three categories CIN 1, CIN 2, and CIN 3, using microscopical criteria. The relation between grades of cervical intraepithelial neoplasia (CIN) and its fractal dimension was investigated to establish a basis for an objective diagnosis using the method proposed. Methods Classical evaluation of the tissue samples was performed by an experienced gynecologic pathologist. Tissue samples were scanned and saved as digital images using Aperio scanner and software. After image segmentation the box counting method as well as multifractal methods were applied to determine the relation between fractal dimension and grades of CIN. A total of 46 images were used to compare the pathologist's neoplasia grades with the predicted groups obtained by fractal methods. Results Significant or highly significant differences between all grades of CIN could be found. The confusion matrix, comparing between pathologist's grading and predicted group by fractal methods showed a match of 87.1%. Multifractal spectra were able to differentiate between normal epithelium and low grade as well as high grade neoplasia. Conclusion Fractal dimension can be considered to be an objective parameter to grade cervical intraepithelial neoplasia. PMID:25302712

  10. Cytological diagnosis of tuberculous cervicitis: A case report with review of literature

    OpenAIRE

    Kalyani, R; S R Sheela; M Rajini

    2012-01-01

    Tuberculosis of cervix is a rare disease. Tuberculosis usually affects women of childbearing age, indicating hormone dependence of infection. The patient presents with menstrual irregularities, infertility or vaginal discharge. Cervical lesions presents as papillary/vegetative growth or ulceration mimicking cervical cancer. Cervical Papanicolaou (Pap) smear plays an important role in diagnosing the disease by non-invasive technique in which the presence of epithelioid cells and Langhan′s type...

  11. The cytological diagnosis and prognosis of malignization of cervical epithelium of uterus in pregnant women.

    OpenAIRE

    Sumenko V.V.

    2007-01-01

    The investigation is dedicated to the study of the pathological alterations of cervical epithelium of uterus, proliferative activity in the lesion focuses and the improvement of cytological diagnosis of the state of cervical epithelium of uterus. The correlation between the proliferative processes in abnormal epithelium of uterine cervix and some pathological factors of the pregnant women organism was studied. The criteria for prognosis of the cervical epithelial dysplasia in pregnant women w...

  12. Percutaneous vertebroplasty for eosinophilic granuloma of the cervical spine in a child

    Energy Technology Data Exchange (ETDEWEB)

    Tan, Hua-Qiao; Li, Ming-Hua; Wu, Chun-Gen; Gu, Yi-Feng; Zhang, He; Fang, Chun [Shanghai Sixth People' s Hospital affiliated to Shanghai Jiao Tong University, Department of Radiology, Shanghai (China)

    2007-10-15

    We report a case of eosinophilic granuloma at the fourth cervical vertebra in a 10-year-old girl presenting with a 1-month history of cervical pain and stiffness. This lesion was histologically diagnosed by needle biopsy and then treated by percutaneous vertebroplasty. After the procedure, the cervical pain and stiffness resolved rapidly. The height of the vertebral body remained stable without further collapse over a 6-month follow-up period. (orig.)

  13. Cervical sympathetic chain schwannoma: a case report

    OpenAIRE

    Inès Nacef; Skander Kedous; Zied Attia; Slim Touati; Said Gritli

    2012-01-01

    Nerve tumors arising from the sympathetic chain are uncommon slow-growing tumors and represent a diagnosis challenge. Their malignant degeneration is rare. Definitive pre-operative diagnosis may be difficult as investigations are not usually helpful. We report the case of a 23-year old woman who presented with an asymptomatic solitary left cervical swelling. She was evaluated with sonography and computed tomography. Complete surgical excision of the lesion was carried out and histologic exami...

  14. Abrasive supply for ancient Egypt revealed

    International Nuclear Information System (INIS)

    In the framework of the major research scheme 'Synchronization of Civilizations in the Eastern Mediterranean Region in the 2nd Millennium B.C' instrumental neutron activation analysis (INAA) was used to determine 30 elements in pumice from archaeological excavations to reveal their specific volcanic origin. In ancient time, the widespread pumiceous products of several eruptions in the Aegean region have been used as abrasive tools and were therefore popular trade objects. The correlation of such archaeological findings to a specific eruption of known age would therefore allow to certify a maximum age of the respective stratum ('dating by first appearance'). Pumices from the Aegean region can easily be distinguished by their trace element distribution patterns. This has been shown by previous studies of the group. The elements Al, Ba, Ca, Ce, Co, Cr, Cs, Dy, Eu, Fe, Hf, K, La, Lu, Mn, Na, Nd, Rb, Sb, Sc, Sm, Ta, Tb, Th, Ti, U, V, Yb, Zr and Zn were determined in 16 samples of pumice lumps from excavations in Tell-el-Dab'a and Tell-el-Herr (Egypt). Two irradiation cycles and five measurement runs were applied. To show the accuracy of the results obtained, typical samples of the most important pumice sources in the Aegean region, particularly from Milos, Nisyros, Kos and Thera were analyzed together with the Egyptian samples of unknown origin. A reliable identification of the samples is achieved by comparing these results to the database compiled in previous studies. The geographical positions of these islands are shown. Within the error range, most of the elements determined in typical representatives of Milos, Nisyros, Kos and Santorini were in perfect agreement with values from the literature. On the basis of the Cluster graphics presented, it is possible to relate unknown pumice to its primary source, just by comparing the relation of a few elements, like Ta-Eu and Th-Hf. One concludes that all samples except one can be related to the Minoan eruption of Thera

  15. Comparative pulmonary toxicity of 6 abrasive blasting agents

    Energy Technology Data Exchange (ETDEWEB)

    Hubbs, A.F.; Minhas, N.S.; Jones, W.; Greskevitch, M.; Battelli, L.A.; Porter, D.W.; Goldsmith, W.T.; Frazer, D.; Landsittel, D.P.; Ma, J.Y.C.; Barger, M.; Hill, K.; Schwegler-Berry, D.; Robinson, V.A.; Castranova, V. [Center for Disease Control and Prevention, Morgantown, WV (USA). Health Effects Laboratory Division

    2001-05-01

    Inhalation of silica dust is associated with pulmonary fibrosis, Therefore, substitute abrasive materials have been suggested for use in abrasive blasting operations. To date, toxicological evaluation of most substitute abrasives has been incomplete. Therefore, the objective of this study was to compare the pulmonary toxicity of a set of substitute abrasives (garnet, staurolite, coal slag, specular hematite, and treated sand) to that of blasting sand. Rats were exposed to blasting sand or an abrasive substitute by intratracheal instillation and pulmonary responses to exposure were monitored 4 weeks postexposure. Pulmonary damage was monitored as lactate dehydrogenase (LDH) in the acellular lavage fluid. Pulmonary inflammation was evaluated from the yield of polymorphonuclear leukocytes (PMN) obtained by bronchoalveolar lavage. The activity of alveolar macrophages was determined by measuring: zymosan-stimulated chemiluminescence. Blasting sand caused lung damage and showed histologic evidence for inflammation and fibrosis, Garnet, staurolite, and treated sand exhibited toxicity and inflammation that were similar to blasting sand. while coal slag caused greater pulmonary damage and inflammation than blasting sand, In contrast, specular hematite did not significantly elevate LDH or PMN levels and did not stimulate macrophage activity 4 weeks postexposure.

  16. Rock Abrasion Tool Exhibits the Deep Red Pigment of Mars

    Science.gov (United States)

    2006-01-01

    During recent soil-brushing experiments, the rock abrasion tool on NASA's Mars Exploration Rover Spirit became covered with dust, as shown here. An abundance of iron oxide minerals in the dust gave the device a reddish-brown veneer. Investigators were using the rock abrasion tool to uncover successive layers of soil in an attempt to reveal near-surface stratigraphy. Afterward, remnant dirt clods were visible on both the bit and the brush of the tool. Designers of the rock abrasion tool at Honeybee Robotics and engineers at the Jet Propulsion Laboratory developed a plan to run the brush on the rock abrasion tool in reverse to dislodge the dirt and return the tool to normal operation. Subsequent communications with the rover revealed that the procedure is working and the rock abrasion tool remains healthy. Spirit acquired this approximately true-color image with the panoramic camera on the rover's 893rd sol, or Martian day (July 8, 2006). The image combines exposures taken through three of the camera's filters, centered on wavelengths of 750 nanometers, 530 nanometers, and 430 nanometers.

  17. Interleukin-10 and Fas polymorphisms and susceptibility for (pre)neoplastic cervical disease

    NARCIS (Netherlands)

    Zoodsma, M; Nolte, IM; Schipper, M; Oosterom, E; Van der Steege, G; De Vries, EGE; Te Meerman, GJ; Van der Zee, AGJ

    2005-01-01

    Infection with oncogenic types of human papillomavirus (HPV) is the main causal factor of cervical cancer and its precursor lesion (cervical intraepithelial neoplasia [CIN]). Cellular immunity may be critical in the elimination of HPV-harboring cells. Interleukin-10, a T-helper type 2 cytokine, has

  18. Frequencies and role of regulatory T cells in patients with (pre)malignant cervical neoplasia

    NARCIS (Netherlands)

    Visser, J.; Nijman, H. W.; Hoogenboom, B.-N.; Jager, P.; van Baarle, D.; Schuuring, E.; Abdulahad, W.; Miedema, F.; van der Zee, A. G.; Daemen, T.

    2007-01-01

    Oncogenic human papillomavirus (HPV)-infection is crucial for developing cervical cancer and its precursor lesions [cervical intraepithelial neoplasia (CIN)]. Regulatory T cells (T-regs) might be involved in the failure of the immune system to control the development of HPV-induced cancer. We invest

  19. Automated identification of diploid reference cells in cervical smears using image analysis.

    NARCIS (Netherlands)

    Laak, J.A.W.M. van der; Siebers, A.G.; Cuijpers, V.M.J.I.; Pahlplatz, M.M.M.; Wilde, P.C.M. de; Hanselaar, A.G.J.M.

    2002-01-01

    BACKGROUND: Acquisition of DNA ploidy histograms by image analysis may yield important information regarding the behavior of premalignant cervical lesions. Accurate selection of nuclei for DNA measurement is an important prerequisite for obtaining reliable data. Traditionally, manual selection of nu

  20. Microleakage of compomer restorations in primary teeth after preparation with bur or air abrasion.

    Science.gov (United States)

    Aysegül, Olmez; Nurhan, Oztas; Haluk, Bodur; Dilek, Tüfekçioglu

    2005-01-01

    This study compared the degree of marginal leakage of a compomer in Class V cavities of human primary molars prepared by a conventional dental bur and air abrasion with or without acid etching. Fifty-six non-carious extracted primary molars were randomly divided into four groups (n=14) to be prepared by four techniques: Group-1: Bur followed by acid etching: Class V cavity preparations were placed on the buccal surfaces of each tooth using a high-speed handpiece. The preparations were 1.5-mm deep, 3-mm long and 2-mm wide, with the occlusal margin in enamel and the cervical margin extending 0.5 mm below the cementoenamel junction. The preparations were acid etched with 37% phosphoric acid starting at the enamel margins for 30 seconds and rinsed with water for 20 seconds. The preparations were then restored with Compoglass F. 2-Group 2: Bur: The preparations and the treatment procedures were the same as in Group 1, with the exception of 37% phosphoric acid application. Group 3: Air abrasion followed by acid etching: Class V cavity preparations were placed on the buccal surfaces of each tooth using a handpiece of an air-abrasive system (PrepStart, Danville Engineering). The system was supplied with dry compressed air at 80 psi. In all tests, the air-abrasion system was operated with an 80 degrees-angle handpiece tip and 50-mm aluminum oxide particles. A tip with a 0.38-mm inner diameter was used at a 2-mm distance. The treatment procedures were the same as in Groups 1 and 2. Group 4: Air abrasion: The preparations and treatment procedures were the same as in Group 3, with the exception of 37% phosphoric acid. After finishing the restorations, the teeth were stored in distilled water at 37 degrees C for 24 hours. The samples were thermocycled for 500 cycles between 5 degrees C and 55 degrees C with a dwell time of 30 seconds. The samples were then immersed in 0.5 percent basic fuchsin dye for 24 hours at 37 degrees C. The surface-adhered dye was then rinsed in tap

  1. Primary Investigation on the Relations between Human Papilloma Virus Infection and Imbalance of Vaginal Microecosystem as well as Cervical Lesions%人乳头状瘤病毒(HPV)感染和阴道微生态失调与宫颈病变关系的初探

    Institute of Scientific and Technical Information of China (English)

    胡灵; 杨云成

    2016-01-01

    Objective To understand whether human papilloma virus (HPV)infection is related to imbalance of vaginal microecosystem,as well as whether the possibility of microecosystem imbalance increases with the aggravation in cervical lesion grade.Methods 206 cases of inpatients or outpatients in our hospital during January,2013 to December,2015 were selected.All patients received cervical pathological examination (either biopsy or uterine specimen),HPV and leucorrhoea examination,and were divided into four groups in accordance with the results of pathological examination,which were group A:normal cervix;group B:low-grade lesion;group C:high-grade lesion;group D:cervical cancer.HPV infection cases,uninfected cases,as well as vaginal microecosystem imbalance cases of each group were counted.Results There were 40 cases in group A,among which 2 were HPV positive (5%),with no cases of vaginal microecosystem imbalance;and 38 were HPV negative (95%),with 6 of which of vaginal microecosystem imbalance (16%).Group B was comprised of 22 cases, among which 5 were HPV positive (23%),with 3 of vaginal microecosystem imbalance (60%);and 17 were HPV negative (77%), with 5 of vaginal microecosystem imbalance (29%).Group C was composed of 95 cases,among which 76 were HPV positive (80%),with 65 of vaginal microecosystem imbalance (80%);and 19 were HPV negative (2%),with 5 of vaginal microecosystem imbalance (26%).Group D consisted of 49 cases,among which 44 were HPV positive (90%),with 39 of vaginal microecosystem imbalance (89%);and 5 were HPV negative (10%),with 2 of vaginal microecosystem imbalance (4%).The detection rate of HPV infection gradually increased with the aggravation in lesion grade,and the number of HPV infection and uninfected cases had significant differences through chi-square test(X2=98.341,P 0.05).Conclusion The degree of vaginal microecosystem imbalance gradually increased with the increase in the aggravation of cervical lesion grade which was induced by HPV infection

  2. Study of abrasive resistance of composites for dental restoration by ball-cratering

    OpenAIRE

    Antunes, P. Vale; Ramalho, A

    2003-01-01

    Two-body abrasion occurs in the mouth whenever there is tooth-to-tooth contact. This is what most dentists call attrition. Abrasive wear may also occur when there is an abrasive slurry interposed between two surfaces, such that the two solid surfaces are not actually in contact, this is called three-body abrasion, with food acting as the abrasive agent, and occurs in the mouth during mastication. Abrasion is the key physiological wear mechanism that is present in dental materials during norma...

  3. Comminution of Mica by Cavitation Abrasive Water Jet

    Institute of Scientific and Technical Information of China (English)

    GUO Chu-wen; LIU Lin-sheng; HAN Dong-tai; LI Ai-min; ZHANG Dong-hai

    2003-01-01

    The comminution of mica with an abrasive water jet is mainly based on three knids of effects, that is, high-speed collision, cavitating effect and shearing effect. Cavitation abrasive water jet was applied for the comminution of mica because cavitation abrasive water jet can make full use of the three effects mentioned above. Besides high speed impacting among particles,cavitation and shearing were also enhanced due to the divergent angle at the outlet of the cavitation nozzle.A JME-200CX transmission electron microscope was used for observing the size distribution of particles.Variance analysis on the experimental results indicates that the effect of cavitation is much more significant than that of collision.The effect of pressure on comminution results becomes less with the decrease of the particle size.

  4. Abrasive wear and surface roughness of contemporary dental composite resin.

    Science.gov (United States)

    Han, Jian-min; Zhang, Hongyu; Choe, Hyo-Sun; Lin, Hong; Zheng, Gang; Hong, Guang

    2014-01-01

    The purpose of this study was to evaluate the abrasive wear and surface roughness of 20 currently available commercial dental composite resins, including nanofilled, supra-nanofilled, nanohybrid and microhybrid composite resins. The volume loss, maximum vertical loss, surface roughness (R(a)) and surface morphology [Scanning electron microscopy (SEM)] were determined after wear. The inorganic filler content was determined by thermogravimetric analysis. The result showed that the volume loss and vertical loss varied among the materials. The coefficients of determination (R(2)) of wear volume loss and filler content (wt%) was 0.283. SEM micrographs revealed nanofilled composites displayed a relatively uniform wear surfaces with nanoclusters protrusion, while the performance of nanohybrid composites varied. The abrasive wear resistance of contemporary dental composite resins is material-dependent and cannot be deduced from its category, filler loading and composite matrix; The abrasive wear resistance of some flowable composites is comparable to the universal/posterior composite resins.

  5. Cervical Microbiome and Cytokine Profile at Various Stages of Cervical Cancer: A Pilot Study

    Science.gov (United States)

    Bahena-Román, Margarita; Téllez-Sosa, Juan; Martínez-Barnetche, Jesús; Cortina-Ceballos, Bernardo; López-Estrada, Guillermina; Delgado-Romero, Karina; Burguete-García, Ana I.; Cantú, David; García-Carrancá, Alejandro; Madrid-Marina, Vicente

    2016-01-01

    Cervical cancer (CC) is caused by high-risk human papillomavirus persistence due to the immunosuppressive tumor microenvironment mediated by cytokines. Vaginal microbiota determines the presence of certain cytokines locally. We assessed the association between cervical microbiota diversity and the histopathological diagnosis of each stage of CC, and we evaluated mRNA cervical expression levels of IL-4, IL-6, IL-10, TGF-β1, TNF-α and IFN-γ across the histopathological diagnosis and specific bacterial clusters. We determined the cervical microbiota by high throughput sequencing of 16S rDNA amplicons and classified it in community state types (CST). Mean difference analyses between alpha-diversity and histopathological diagnosis were carried out, as well as a β-diversity analysis within the histological diagnosis. Cervical cytokine mRNA expression was analyzed across the CSTs and the histopathological diagnoses. We found a significant difference in microbiota's diversity in NCL-HPV negative women vs those with squamous intraepithelial lesions (SIL) and CC(p = 0.006, p = 0.036).When β-diversity was evaluated, the CC samples showed the highest variation within groups (p<0.0006) and the largest distance compared to NCL-HPV negative ones (p<0.00001). The predominant bacteria in women with normal cytology were L. crispatus and L. iners, whereas for SIL, it was Sneathia spp. and for CC, Fusobacterium spp. We found higher median cervical levels of IL-4 and TGF-β1 mRNA in the CST dominated by Fusobacterium spp. These results suggest that the cervical microbiota may be implicated in cervical cancer pathology. Further cohort studies are needed to validate these findings. PMID:27115350

  6. 液基细胞学制片技术与人乳头瘤病毒L1壳蛋白检测对子宫颈病变诊断及进展风险评估%Liquid based cytology test and human papilloma virus L1 Nuclecapsid protein detection in di-agnosis of cervical lesions and its progress

    Institute of Scientific and Technical Information of China (English)

    陈灵芝

    2016-01-01

    Objective:To discuss the clinical value of liquid based cytology test in the diagnosis of cervical lesions and to evaluate the expression and significance of human papilloma virus L1(HPV L1)protein in exfoliative cells of different cervical lesions.Methods:A total of 1400 cases visiting gynaecological clinics of our hospital from January 2009 to August 2013 were se-lected and were given liquid based cytology test (LCT)examination and HPV L1 protein expression detection in exfoliative cells.Meanwhile,cervical biopsy pathology results were taken as baseline for make a comparison.Results:(1)Out of 1400 ca-ses,there were 30 cases with LCT(+),accounting for 2.1%,18 cases with HPV L1 protein detection(+),accounting for 1. 29%;8 cases with LCT and HPV L1 protein detection(+),1360 cases with LCT and HPV L1 protein detection(-).(2) Pathological tissue biopsy was performed on 30 cases of patients with LCT(+),results showed that there were 23 cases with positive result.Pathological tissue biopsy was performed on 18 cases of patients with HPV L1 protein(+),results showed that 14 cases with positive result.Among 1360 cases with LCT and HPV L1 protein detection(-),497 cases accepted the patho-logical biopsy,and the results showed that 1 case presented(+),accounting for 0.2%.Totally 545 cases accepted the patholog-ical biopsy,among whom,38 cases presented(+),accounting for 6.97%;(3)Sensitivity and specificity of LCT combined with HPV L1 protein detection were higher than single-method detection,and difference had statistical significance(P <0.05).Con-clusion:Liquid based cytology test combined with HPV L1 protein detection could improve the diagnostic rate of cervical le-sions,and is expected effective method in the early clinical screening of cervical neoplasia lesions.%目的::探讨液基细胞学制片技术对诊断子宫颈病变的价值,评价人乳头瘤病毒 L1(HPV L1)在子宫颈不同病变脱落细胞中的表达差异及意义.方法:将河

  7. DNA甲基转移酶1与甲基-CpG-结合蛋白2异常表达在宫颈病变中的作用及相互关系%Effect of DNA methyltransferase 1 and methyl-CpG-binding protein 2 abnormal expression on cervical lesions and related interaction

    Institute of Scientific and Technical Information of China (English)

    康慧杰; 王金桃; 高晨菲; 白丽霞; 丁玲; 许娟; 吴婷婷; 白兰

    2013-01-01

    目的 探讨DNA甲基转移酶l(DNMT)和甲基-CpG-结合蛋白2(MeCP2)与宫颈癌发生发展的关系及其在宫颈癌变中的相互作用.方法 选择经病理学确诊的宫颈鳞状细胞癌(SCC)患者74例、低度宫颈上皮内瘤样变(CINⅠ)患者52例、高度宫颈上皮内瘤样变(CINⅡ-Ⅲ)患者60例和宫颈炎(CI)患者58例为研究对象.在收集全部对象人口学特征、人乳头瘤病毒感染、生殖因素等相关资料同时,检测DNMT1和MeCP2蛋白(Western blot法)和mRNA (real-time PCR法)的相对表达量.采用SPSS 17.0分析软件,计算相关资料的t检验、x2检验、因素与疾病之间关联强度指标(OR值及其95%CI)及其交互作用.结果 随着宫颈病变的加重,DNMT1和MeCP2蛋白表达水平逐渐增高(H=94.33,P<0.001;F=21.580,P<0.001),DNMTl和MeCP2 mRNA的表达水平亦逐渐增高(F=4.758,P=0.003; F=7.804,P<0.001).相关分析表明,DNMT1与MeCP2(r=0.287,P<0.001)和rnRNA(r=0.179,P=0.005)表达均存在正相关关系.交互作用分析显示,DNMT1与MeCP2蛋白和mRNA高表达在SCC组和CINⅡ-Ⅲ组存在正相加交互作用.结论 DNMT1及MeCP2高表达可增加宫颈癌和癌前病变发生的风险,两者在CIN Ⅱ-Ⅲ组和SCC组存在正相加交互作用.%Objective To explore the effect of DNA methyltransferase 1 (DNMT1) and methyl-CpG-binding protein 2 (MeCP2) on cervical cancer and cervix precancerous lesion.Methods 74 patients with cervix squamous cell carcinoma (SCC),52 patients with cervical intraepithelial neoplasm Ⅰ (CIN Ⅰ),60 patients with cervical intraepithelial neoplasm Ⅱ-Ⅲ(CIN Ⅱ-Ⅲ) and 58 patients with histologically diagnosed cervix inflammation (CI),were included in this study.Information as demography,reproductive history,life style,HPV infection were collected.Western Blot were used to detect the expression of DNMT1 protein and MeCP2 protein.Real-time PCR was used to detect the expression of DNMT 1 and MeCP2 mRNA.Results Levels of DNMT1 and MeCP2

  8. Genotype detection analysis of cervical lesions in patients with human papillomavirus in Luliang City%吕梁地区宫颈病变患者人乳头状瘤病毒基因型的检测分析

    Institute of Scientific and Technical Information of China (English)

    常新剑

    2012-01-01

    目的 调查吕梁地区21种人乳头状瘤病毒基因型的检测分析.方法 收集568例女性宫颈病变患者宫颈分泌物中的脱落细胞,应用人乳头状瘤病毒导流杂交快速基因分型技术检测21种人乳头状瘤病毒亚型,包括13种高危亚型(16,18,31,33,35,39,45,51,52,56,58,59和68型)、5种低危亚型(6,11,42,43和44型)和3种中国人群常见亚型(53,66和CP8304型);分析21种基因型的流行病学特征.结果 人乳头状瘤病毒感染率41.9%,单一感染率58.4%,混合性感染率39.9%.21种基因型中,高危型以16,53型为主,其次是52,58型,低危型以6,11型为主;人乳头状瘤病毒16型的感染率居首位.结论 本地区21种人乳头状瘤病毒基因型的检测分析资料对人乳头状瘤病毒疫苗研究、应用及其感染的防治有重要意义.%Objective: To investigate the molecular epidemiologic characteristics of 21 genotypes of human papillomavirus in women with cervical disease in LuLJang. Methods; A total of 568 samples were collected from female patients with cervical disease in Henan. Twenty - one genotypes of human papillomavirus were tested by hybrid Max, and studied the molecular epidemiologic characteristics of human papillomavirus subtypes, including 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 6, 11, 42, 43, 44, 53, 66, and CP8304. Results; The total positive rate of 21 genotypes of human papillomaviruswas 41. 9% . The positiverate was 58. 4% of single human papillomavirus genotypes, and 39. 9% of mixed infection. Among 21 genotypes of human papillomavirus, the main high - risk genotypes were human papillomavirus 16 and 53, and low - risk genotypes were 6 and 11. The infectious rate of human papillomavirus 16 ranked first. Conclusion; The information on the molecular epidemiology of human papillomavirus would play an important role in the study and application of vaccine, and in the prevention and therapy of human papillomavirus infection.

  9. An instrument for measuring abrasive water jet diameter

    OpenAIRE

    Junkar, Mihael; Lebar, Andrej; Orbanić, Henri

    2015-01-01

    In order to improve the accuracy of abrasive water jet (AW) machining the precise value of the jet diameter has to be known. Because of an aggressive environment caused by high velocity abrasive grains, the diameter is not easily measured. That is why a measuring device consisting of a load cell and a wear resistant probe was developed. The device measures the force of the jet while it passes over the edge of the probe. If the feed rate of the jet is constant and the time needed for jet to pa...

  10. Evaluation of dentifrice abrasion on human dentin and teeth enamel

    International Nuclear Information System (INIS)

    Radiometric method is applied to the dentifrice abrasion indicators evaluation: radioactive dentin (RDA) and radioactive enamel abrasion (REA). The dentifrices analysed presented RDA values varying from 95 to 13 and REA values from 7.1 to 0.7, showing a good precision, generally with relative standard deviations lower than 15.4%. Also, the dentifrices were classified as having low and medium cleaning potential. A correlation between RDA and REA values was obtained, i.e. dentifrices with high RDA values presented high REA ones. (author). 6 refs., 3 tabs

  11. Design and manufacturing of abrasive jet machine for drilling operation

    Directory of Open Access Journals (Sweden)

    Mittal Divyansh

    2016-01-01

    Full Text Available Wide application of Abrasive Jet Machine (AJM is found in machining hard and brittle materials. Machining of brittle materials by AJM is due to brittle fracture and removal of micro chips from the work piece. Embedment of the abrasive particles in the brittle materials results in decrease of machining efficiency. In this paper design and manufacturing of AJM has been presented. Various parts of AJM have been designed using ANSYS 16.2 software. The parts are then manufactured indigenously as per designed parameters. The machine fabricated in this work will be used further for process optimization of AJM parameters for machining of glass and ceramics.

  12. 人端粒酶RNA基因荧光原位杂交检测在宫颈细胞学检查中的应用%Detection of human telomerase RNA component gene by fluorescent in situ hybridization for screening of cervical lesions

    Institute of Scientific and Technical Information of China (English)

    高莉; 朱炎; 刘素萍; 高妍; 朱明华

    2011-01-01

    目的 探讨人类染色体端粒酶RNA基因(hTERC)扩增的荧光原位杂交(FISH)检测在宫颈脱落细胞防癌筛查中的意义.方法 收集2008年2-10月上海长海医院门诊就诊146例患者宫颈液基细胞学样本,应用间期双色FISH技术检测hTERC基因的扩增情况,并与细胞学和组织学结果进行对照.结果 杂交成功120例(细胞学阴性20例、细胞学阳性100例).hTERC基因扩增的阳性率与细胞学等级成正相关(r=0.465,P<0.01),与组织学等级成正相关(r=0.610,P<0.01),各级的阳性率分别为:炎性病变0(0/13)、宫颈上皮内瘤样病变(CIN)Ⅰ级2 8.6%(6/21)、CINⅡ级11/18、CINⅢ级75.0%(18/24)、鳞状细胞癌91.7%(22/24);FISH方法检出高级别(CINⅡ/Ⅲ级)以上病变的敏感性为77.3%(51/66),特异性为82.4%(28/34).阳性预测值89.5%,阴性预测值65.1%,低级别(CIN Ⅰ级)以下病变的hTERC阳性率与高级别以上病变相比差异有统计学意义(x2=32.550,P<0.01).结合hTERC高倍扩增(信号数>4)的出现,检出高级别以上病变的敏感性提高到81.2%.结论 hTERC基因扩增的FISH检测有助于辅助细胞学诊断,提高高危病变的检出率.实验结果判断除参照阈值之外,还应结合hTERC基因的扩增类型,出现高倍扩增亦提示高级别以上病变.%Objective To investigate the value of fluorescence in situ hybridization (FISH)detection of human telomerase RNA component ( hTERC ) gene amplification in screening of cervical lesions.Methods A total of 146 post-thinPrep cytology test (TCT) samples were analyzed using FISH by two-color interphase probe targeting hTERC gene at chromosome 3q26 and the data were compared with the cytological and histological results. Results FISH analysis was successful in 120 cases (20 cases of normal and 100 abnormal cases by TCT). Gene amplification of hTERC by FISH had a positive correlation with the cytological (r = 0. 465, P < 0. 01 ) and histological grade results ( r = 0. 610, P < 0

  13. The study of thinprep cytology test combined truscreen in early diagnosis cervical precancerous lesions%膜式薄层液基细胞学检查联合初善仪在子宫颈癌前病变诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    张小鹏; 罗新; 王晓玉

    2012-01-01

    目的 探讨膜式薄层液基细胞学检查(thinprep cytologic test,TCT)联合初善仪(truscreen,TS)在子宫颈癌前病变诊断中的应用价值.方法 对30例阴道分泌物异常的患者同时行TCT和TS检查,再行阴道镜检查及组织活检,以组织病理学检查结果作为诊断的金标准.比较2种检查方法单独及联合使用与组织病理学检查结果的吻合程度.结果 30例患者中有25例病理学诊断为子宫颈上皮内瘤变1级及以上病变,为真阳性.TCT测试细胞学意义不明的不典型鳞状上皮细胞以上的阳性19例(63.3%),假阴性6例,漏诊率20.0%,真阳性16例,敏感度84.2%.TS测试阳性18例(60.0%),假阴性7例,漏诊率23.3%,真阳性14例,敏感度77.8%.TCT联合TS测试阳性27例(90.0%),假阴性1例,漏诊率3.3%,真阳性24例,敏感度88.9%.TCT联合TS检测子宫颈癌前病变的阳性率与病理学检查的阳性率分别为90.0%和83.3%,差异无统计学意义(P>0.05).结论 TCT联合TS检查诊断子宫颈癌前病变的准确性明显优于任一单项检查,其漏诊率显著下降.%Objective: To investigate the application value of thinprep cytology test(TCT) combined truscreen(TS) in early diagnosis cervical precancerous lesions. Methods:Thirty patients with abnormal vaginal secretions were detected under TCT and TS,and then vaginal tissue biopsies were tested under colposcopy. The results of pathological examination acted as the gold standard of diagnosis. The match degrees of single and combined using methods and histopathological examination results were compared. Results: The results of histopathological examination of 25 cases were cervical intraepithelial neoplasia grade 1 or above. Nineteen cases(63. 3% ) were ASCUS cytology positive,6 cases were false negative,16 cases were true postive,the rate of fail to diagnosis was 20.0% ,and the sensitiveness degree was 84. 2% under TCT. Compared with TCT results,the data were 18 cases(60. 0% ) ,7 cases, 14 cases,23. 3% and

  14. Short-term risk of cervical intraepithelial neoplasia grades 2 and 3 for women with normal cytology and human papillomavirus infection Riesgo a corto plazo de lesiones intraepiteliales cervicales grados 2 y 3 en mujeres con citología vaginal normal e infección por el virus del papiloma humano

    Directory of Open Access Journals (Sweden)

    Gustavo Hernández-Suárez

    2010-12-01

    Full Text Available OBJECTIVE. To assess the risk of cervical intraepithelial neoplasia grades 2, 3 or higher (CIN 2/3+ for women with normal cytology and concurrent high-risk human papillomavirus infection (HR-HPV. MATERIAL AND METHODS. We examined 2 200 women every 6 months for an average of 9 years. Cervical smears and samples for HPV DNA were obtained at each visit. Absolute risk of subsequent CIN2/CIN3+ was estimated using the Kaplan-Meier method. RESULTS. The absolute risk of CIN2/CIN3+ among HR-HPV-positive women with normal Pap smear results was 1.06% (95%CI, 0.57-2.20, 5 times higher the risk among all women with normal Pap smears (0.20%; 95%CI, 0.12-0.32 but 7 times lower than that for women with HR-HPV infection and LSIL (7.24%; 95%CI, 3.78-15.2. CONCLUSION. Short-term absolute risk of CIN2/3+ after a normal Pap smear with concurrent HR-HPV infection is low (~1%, suggesting that the HR-HPV test has limited utility in short-term clinical decision-making for women with normal cytology.OBJETIVO. Evaluar el riesgo a corto plazo de neoplasia intraepitelial cervical de alto grado (CIN2/CIN3+ en mujeres con citologí-a cervicouterina normal e infección por virus del papiloma humano de alto riesgo (HR-HPV. MATERIAL Y MÉTODOS. Cohorte prospectiva de 2200 mujeres evaluadas cada seis meses durante 9 años en promedio. En cada visita se tomó muestra cervical para extendido y detección de HPV DNA. El riesgo absoluto de CIN2/CIN3+ a la siguiente visita fue calculado utilizando el método de Kaplan-Meier. RESULTADOS. En mujeres con citologí-a normal e infección concomitante por HR-HPV el riesgo absoluto de presentar CIN2/CIN3+ fue de 1.06% (95%CI, 0.57-2.20. Este riesgo fue cinco veces mayor al observado en todas las mujeres con citologí-a normal (0.20%; 95%CI, 0.12-0.32 pero siete veces menor que el observado en mujeres con lesiones intraepiteliales escamosas de bajo grado con infección concomitante (7.24%; 95%CI, 3.78-15.2. CONCLUSIÓN. El riesgo absoluto de

  15. Get Tested for Cervical Cancer

    Science.gov (United States)

    ... Cervical Cancer Print This Topic En español Get Tested for Cervical Cancer Browse Sections The Basics Overview ... be cured. How often should I get screened (tested)? How often you should get screened for cervical ...

  16. Cervical cancer - screening and prevention

    Science.gov (United States)

    Cancer cervix - screening; HPV - cervical cancer screening; Dysplasia - cervical cancer screening ... Almost all cervical cancers are caused by HPV (human papilloma virus). HPV is a common virus that spreads through sexual contact. Certain ...

  17. Treatment Option Overview (Cervical Cancer)

    Science.gov (United States)

    ... Cancer Prevention Cervical Cancer Screening Research Cervical Cancer Treatment (PDQ®)–Patient Version General Information About Cervical Cancer ... Certain factors affect prognosis (chance of recovery) and treatment options. The prognosis (chance of recovery) depends on ...

  18. Cervical Total Disc Arthroplasty

    OpenAIRE

    Basho, Rahul; Hood, Kenneth A.

    2012-01-01

    Symptomatic adjacent segment degeneration of the cervical spine remains problematic for patients and surgeons alike. Despite advances in surgical techniques and instrumentation, the solution remains elusive. Spurred by the success of total joint arthroplasty in hips and knees, surgeons and industry have turned to motion preservation devices in the cervical spine. By preserving motion at the diseased level, the hope is that adjacent segment degeneration can be prevented. Multiple cervical disc...

  19. Methods of diagnosis in cervical neoplasia

    Directory of Open Access Journals (Sweden)

    Mariana Bratu

    2010-06-01

    Full Text Available The early screening of a precancerous lesion that can develop spontaneously into a cancerous lesion thatis first non-invasive and then invasive. Materials and methods. The study group was made of 1485 patients who were hospitalized between 2001 and 2009 in the Section of Obstetrics and Gynecology of ?Sf. Apostol Andrei? Emergency Hospital in Gala?i. The patients filled in a questionnaire on a sheet of paper in a freely consented way and they were diagnosed with cervical cancer by a cytologic and colposcopic examination, biopsy and other complementary examinations. Results and discussions. It is obvious that, even if cervical carcinoma benefits now from a remarkable methodology of detecting the early forms and even the precursor lesions, in our country this lesion has an importantfrequency and even represents a public health issue with important social-economic implications; in most cases it affects people in full physical and procreative activity; it implies a laborious therapy, long time hospitalization and high costs. The risk of getting infected with HPV(human papilloma virus is at least 50% for the sexually active people for the rest of their lives. Although most infections eliminate with the help of our own immunity, the people who are infected are not aware of HPV presence and they can spread the virus. When our own immune system cannot eliminate the infection, thepersistence of the viral oncogenic strains from the cervical mucosa can lead to the appearance of precancerous lesions.The cytological examination is a simple and fast method that is also cheap and reliable being considered the mosteffective technique for preventing and detecting the precancerous conditions of the cervix, which, treated correctly, canoffer a primary prevention of cervical cancer. The diagnostic certainty was accomplished exclusively by histopathologyof the material obtained during biopsy. Conclusions. We consider that the patients with cervical cancer, no matter

  20. Association of traffic-related hazardous air pollutants and cervical dysplasia in an urban multiethnic population: a cross-sectional study

    OpenAIRE

    Scheurer, Michael E.; Danysh, Heather E.; Follen, Michele; Philip J Lupo

    2014-01-01

    Background Human papillomavirus (HPV) infection is a necessary cause in the development of cervical cancer; however, not all women infected with HPV develop cervical cancer indicating that other risk factors are involved. Our objective was to determine the association between exposure to ambient levels of common traffic-related air toxics and cervical dysplasia, a precursor lesion for cervical cancer. Methods The study sample consisted of women enrolled in a Phase II clinical trial to evaluat...

  1. Preventing cervical cancer globally.

    Science.gov (United States)

    Schmeler, Kathleen M

    2012-11-01

    Cervical cancer is one of the leading causes of cancer and cancer-related deaths among women worldwide. More than 85% of cases and deaths occur in the developing world where the availability of effective screening is limited. In this issue of the journal, Pierce and colleagues (beginning on page 1273) describe a novel technique using a high-resolution microendoscope (HRME) to diagnose cervical dysplasia. This perspective reviews the limitations of existing cervical cancer screening methods currently in use in low-resource settings and the potential for HRME imaging to contribute to cervical cancer prevention in the developing world.

  2. Abrasion Properties of Steel Fiber Reinforced Silica Fume Concrete According to Los Angeles and Water Abrasion Tests

    OpenAIRE

    Tsan-Ching CHENG; Cheng, An; Huang, Ran; Lin, Wei-Ting

    2014-01-01

    The current study mainly investigated the influence of different tests on the abrasion resistance of concrete mixed with steel fibers and silica fume. The abrasion resistance was assessed at 28, 56 and 91 days on concretes with water-binder ratios of 0.35 and 0.55 where in some mixes silica fume was substituted by 5 % of cement by weight. Steel fibers of 0.5 % and 1.0 % of concrete volume were also added into the test concrete by replacement of coarse and fine aggregates. The results showed t...

  3. Healing effect of Sodium-Sucrose-Octasulfate and EGF on epithelial corneal abrasions in rabbits

    DEFF Research Database (Denmark)

    Johansen, Sven; Heegaard, Steffen; Bjerrum, Kirsten Birgitte;

    1998-01-01

    ophthalmology, Sjögrens syndrome, cornea, abrasion, epidermal growth factor, sodium-sucrose-octasulfate......ophthalmology, Sjögrens syndrome, cornea, abrasion, epidermal growth factor, sodium-sucrose-octasulfate...

  4. 血浆sHLA-G在宫颈癌和癌前病变检测中的应用%The application of plasma soluble human leukocyte antigen in diagnosis of patients with cervical cancer and its precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    张欣; 郑妮; 王传新; 朱成宝; 王丽丽; 杜鲁涛; 王顺

    2011-01-01

    康对照组(U值分别为7.564、4.522、7.429,P均<0.05);CIN Ⅲ级组血浆CA125水平明显高于CIN Ⅰ级组和健康对照组(U值分别为5.871、5.435,P均<0.05).ROC曲线分析sHLA-G的AUC为0.828(95% CI:0.768~0.879),其诊断价值高于SCC-Ag[0.727(95% CI:0.658~0.788);Z=2.294,P<0.05]和CA125[0.705(95%CI:0.636~0.769);Z=2.842,P<0.05];SCC-Ag与CA125的诊断效能比较,差异无统计学意义(Z=0.672,P>0.05).经ROC曲线分析确定sHLA-G诊断临界值为109.6 kU/L时,其敏感度为86.3%,特异度为76.1%,阳性预测值为80.0%,阴性预测值为83.3%,准确性为78.4%.宫颈癌患者血浆sHLA-G水平与FIGO分期及淋巴结转移密切相关(U值分别为6.085、4.451,P均<0.05),而与患者年龄、肿瘤大小、组织形态及细胞分化无关(U值分别为1.274、1.956、1.268、2.719,P均>0.05).结论 血浆sHLA-G水平检测有助于宫颈癌及癌前病变的早期发现,并且可以作为宫颈癌病情进展及淋巴结转移的判断指标.%Objective To explore the application value of plasma sHLA-G in diagnosis of CIN and cervical cancer. Methods The plasma sHLA-G levels were detected by ELISA in 102 cases with cervical cancer( FIGO Ⅰ stage 32 cases, Ⅱ stage 28 cases, Ⅲ stage 25 cases and Ⅳstage 17 cases; tumor size:<4 cm 63 cases and ≥4 cm 39 cases; squamous cell carcinoma 78 cases and adenocarcinoma 24 cases;cell differentiation:well 57 cases, moderate 29 cases and poor 16 cases; lymph nodes metastasis negative64 cases and positive 38 cases ), 72 cases with CIN( Ⅰ grade 21 cases, Ⅱ grade 25 cases and Ⅲ grade26 cases ) and 20 cases of healthy controls. The diagnostic value of sHLA-G and its correlations with clinical parameters were analyzed. Results The plasma levels of sHLA-G were 193.6( 151.3-287.4 ) kU/L in cervical cancer group, 48.3( 34.6-57.2 ) kU/L in CIN Ⅰ group, 91.3( 68.2-118.6 ) kU/L in CIN Ⅱ group, 106.4( 73.8-165.7 ) kU/L in CIN Ⅲ group and 45.2( 38.0-55.5 ) kU/L in health control group.The level of s

  5. Histopathological Correlation of Squamous Cell Abnormalities Detected on Cervical Cytology

    Directory of Open Access Journals (Sweden)

    Remzi ABALI

    2011-05-01

    Full Text Available Objective: To investigate the correlation between cytology and cervical biopsy in patients with squamous cell abnormality on cervical cytology.Material and Method: The cervical smears diagnosed in our clinic between 2005-2008 were reviewed retrospectively. Cases which exhibited squamous cell abnormality (n: 374 were evaluated.Results: The mean age was 45.15±10.78. In the cytopathological results, 256 (68.4% ASC-US, 21 (5.6% ASC-H, 31 (8.2% LSIL, 48 (12.8% HSIL, and 8 (4.8% invasive carcinomas were diagnosed. Histopathological results were 213 (57% nonneoplastic, 85 (22.7% CIN I, 14 (3.7% CIN II, 34(9.0% CIN III and 28 (7.5% invasive squamous cell carcinomas. Including all squamous cell abnormalities, the sensitivity of the smear test in CIN I and higher grade lesions was 56.95% and the false positivity was 43.04%. Excluding ASC-US and ASC-H lesions, the sensitivity of the smear test was 77.31% and the false positivity was 22.68%. After evaluating cervical cytohistopathological correlation, the positive predictive value was found to be 100% in invasive carcinoma, 62% in HSIL and 38% in LSIL.Conclusion: As the grade of cytopathological result increases, the correlation between biopsy and the smear test also increases. The high sensitivity of the cervical smear test for high-grade lesions shows that it is an effective screening test.

  6. Detention of HPV L1 Capsid Protein and hTERC Gene in Screening of Cervical Cancer

    Directory of Open Access Journals (Sweden)

    Huang Bin

    2013-06-01

    Full Text Available   Objective(s: To investigate the expression of human papilloma virus (HPV L1 capsid protein, and human telomerase RNA component (hTERC in cervical cancer and the role of detection of both genes in screening of cervical cancer.   Materials and Methods: A total of 309 patients were recruited and cervical exfoliated cells were collected. Immunocytochemistry was employed to detect HPV L1 capsid protein, and fluorescent in situ hybridization (FISH was performed to detect the hTERC. Results: The expression of HPV L1 capsid protein reduced with the increase of the histological grade of cervical cells and was negatively related to the grade of cervical lesions. However, the expression of hTERC increased with the increase of the histological grade and positively associated with the grade of cervical lesions. The proportion of patients with L1(-/hTERC(+ was higher in patients with histological grade of CIN2 or higher than that in those with histological grade of CIN1. The L1(+/hTERC(- and L1(-/hTERC(- were negatively related to the grade of cervical lesions. L1(-/hTERC(+ was positively associated with the grade of cervical lesions. The L1/hTERC ratio increased. The negative predictive value of both HPV L1 and hTERC was higher than that of HPV L1 or hTERC, but there was no marked difference in the screening efficacy of cervical cancer among HPV L1, hTERC and HPV L1+hTERC. Conclusion: HPV L1 capsid protein and hTERC gene may serve as markers for the early diagnosis and prediction of cervical lesions. The increase in L1/hTERC ratio reflects the progression of cervical lesions to a certain extent.

  7. Comparação entre a colpocitologia oncológica de encaminhamento e a da gravidade das lesões cervicais intra-epiteliais Oncologic colpocytology collected in the public health and reference services in the diagnostic of the severity of intraepithelial cervical lesion

    Directory of Open Access Journals (Sweden)

    Guilherme A Lapin

    2000-04-01

    Full Text Available OBJETIVO: Comparar o resultado da colpocitologia oncológica (CO de encaminhamento com o resultado da CO coletada no serviço de referência, e avaliar as alterações da colposcopia e o resultado da biópsia. MÉTODOS: Foram selecionadas 213 mulheres atendidas de janeiro de 1989 a abril de 1991 de um centro especializado de atenção à saúde da mulher, com seguimento até julho de 1998. Noventa foram encaminhadas por CO sugestiva de lesão induzida pelo vírus do papiloma humano (HPV ou neoplasia intra-epitelial cervical (NIC grau 1 e 123 por CO sugestiva de NIC 2 ou 3. RESULTADOS: Das mulheres encaminhadas por CO HPV/NIC 1, 49% apresentavam NIC 2 ou 3 na CO do serviço. Na colposcopia, 16 não apresentavam lesões suspeitas e em 10 não se via a junção escamo-colunar. Ao diagnóstico histológico, 42 (46% apresentavam NIC 2 ou 3. Das 123 mulheres encaminhadas com CO NIC 2 ou 3, 54% apresentaram NIC 2 ou 3 na CO do serviço. Na colposcopia, 24 mulheres não apresentavam lesões suspeitas e em 12 não se via a junção escamo-colunar. Na histologia, 61 (49% apresentavam NIC 2 ou 3. CONCLUSÕES: A conduta expectante nos casos de CO HPV/NIC 1 deve ser criteriosa, envolvendo profissionais capacitados na coleta do exame, e conscientizar a população para retornos periódicos de controle.OBJECTIVE: The study was designed to compare the Pap smear results, performed on the public health service, with: the results of Pap smear collected on the reference service; the colposcopy and punch biopsy results. METHODS: The total of 213 women assisted at the women's hospital ¾ from January 1989 to April 1991, and followed until July 1998 were selected. Ninety were referred because a Pap smears suggestive of Human Papillomavirus (HPV induced lesion or cervical intraepithelial neoplasia (CIN grade 1, and 123, CIN 2 or 3. RESULTS: Among the 90 women referred because of HPV/CIN 1.49% presented CIN 2 or 3 in the Pap smears performed at this service. At the

  8. Contribuição ao estudo da microinfiltração in vitro de lesões cervicais não cariosas restauradas com diferentes materiais adesivos e técnicas In vitro evaluation of microleakage of noncarious cervical lesions restored with different adhesive materials and techniques

    Directory of Open Access Journals (Sweden)

    Adriana Bona MATOS

    1997-01-01

    Full Text Available A restauração de lesões cervicais não cariosas é um procedimento comum na clínica diária, havendo uma alta incidência de casos de perda da restauração, descoloração marginal e recidiva de cárie. A etiologia desse tipo de lesão é considerada multifatorial. Neste trabalho, avaliou-se a microinfiltração diante das seguintes variáveis: tipo de material utilizado (dois adesivos de quarta geração utilizados em conjunto com duas resinas compostas de tipos diferentes, ©microfillª e híbrida, dois métodos de polimerização dos sistemas adesivos (foto e dual e duas técnicas restauradoras (incremental e do incremento único. Na metodologia deste trabalho, utilizaram-se ciclagem térmica e mecânica, técnica de infiltração com nitrato de prata, cortes seriados e leitura dos resultados através de escala de escores. A microinfiltração foi menor quando se utilizou uma resina composta híbrida comparada à resina ©microfillª. Ao se utilizar o método de polimerização dual do sistema adesivo, a microinfiltração foi menor do que com o método somente fotopolimerizável. A técnica restauradora não exerceu influência sobre o padrão de microinfiltração encontrado nas restaurações de lesões cervicais não cariosasRestoration of noncarious cervical lesions is a usual clinical procedure, and there is a high incidence of restoration loss, marginal discoloration, and recurrent caries. All the factors that play an important role in this process have to be considered when studying this kind of lesion of complex multifactorial etiology. Microleakage was evaluated related to the type of material used (two fourth generation bonding systems used with two types of composite resins: hybrid and microfill, two polymerization methods for bonding systems (photo and dual cure and two restorative techniques (incremental and bulk. This study used thermal and mechanical cycling procedures, infiltration tests with silver nitrate solution

  9. Radiologic Findings of Cervical Varix Developed in Late Pregnancy: Case Report

    Energy Technology Data Exchange (ETDEWEB)

    Park, Young Chan; Kim, Ho Kyun [Catholic University of Daegu College of Medicine, Daegu (Korea, Republic of)

    2006-12-15

    Cervical varix is a rare cause of obstetric hemorrhage which needs accurate diagnosis and prompt management because the lesion may be associated with preterm delivery and maternal complications. Our patient was diagnosed with cervical varix and vaginal bleeding at 32 weeks' gestation, using trans vaginal ultrasound and MRI. Persistent vaginal bleeding and preterm labor led to an emergency cesarean section at 33 weeks' gestation. After delivery, cervical varix was spontaneously resolved in the follow-up trans vaginal ultrasound and CT. We report various radiologic features of cervical varix combined with vaginal bleeding in late pregnancy

  10. Electrical resistivity measurements to predict abrasion resistance of rock aggregates

    Indian Academy of Sciences (India)

    Sair Kahraman; Mustafa Fener

    2008-04-01

    The prediction of Los Angeles (LA) abrasion loss from some indirect tests is useful for practical applications. For this purpose, LA abrasion, electrical resistivity, density and porosity tests were carried out on 27 different rock types. LA abrasion loss values were correlated with electrical resistivity and a good correlation between the two parameters was found. To see the effect of rock class on the correlation, regression analysis was repeated for igneous rocks, metamorphic rocks and sedimentary rocks, respectively. It was seen that correlation coefficients were increased for the rock classes. In addition, the data were divided into two groups according to porosity and density, respectively. After repeating regression analysis for these porosity and density groups, stronger correlations were obtained compared to the equation derived for all rocks. The validity of the derived equations was statistically tested and it was shown that all derived equations were significant. Finally, it can be said that all derived equations can alternatively be used for the estimation of LA abrasion loss from electrical resistivity.

  11. Exaggerated abrasion/erosion of human dental enamel surfaces

    DEFF Research Database (Denmark)

    Westergaard, J; Moe, D; Pallesen, Ulla;

    1993-01-01

    An atypical, rapidly proceeding abrasion/erosion of the labial enamel surfaces of the maxillary and mandibular incisors and canines in a 27-yr-old man is reported. Ultrastructural examination of a replica of the teeth showed a practically structureless enamel surface both at the initial examinati...

  12. Numerical study of agglomerate abrasion in a tumbling mixer

    NARCIS (Netherlands)

    Thanh Nguyen, [No Value; Willemsz, Tofan; Frijlink, Henderik; Maarschalk, Kees van der Voort

    2014-01-01

    A numerical simulation using the Discrete Element Method (DEM) was performed to investigate the phenomena concerning the abrasion and breakage of agglomerates in a diffusion powder mixer. Agglomerates were created by defining a single structure of particles with bonds of different strengths using th

  13. Potential of Air-Propelled Abrasives for Selective Weed Control

    Science.gov (United States)

    Novel forms of selective weed control are needed by many types of growers, but especially organic growers who are restricted from using synthetic herbicides. Abrasive grit made from corn cobs was expelled from a sand blaster at 517 kPa pressure and aimed at seedlings of common lambsquarters and corn...

  14. 29 CFR 1926.303 - Abrasive wheels and tools.

    Science.gov (United States)

    2010-07-01

    ... Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR CONSTRUCTION Tools-Hand and Power § 1926.303 Abrasive wheels and tools. (a) Power. All grinding machines shall be supplied with sufficient power to maintain...

  15. Innovative decontamination technology by abrasion in vibratory vessels

    International Nuclear Information System (INIS)

    Available in abstract form only. Full text of publication follows: The possibility of using conventional vibratory vessel technology as a decontamination technique is the motivation for the development of this project. The objective is to explore the feasibility of applying the vibratory vessel technology for decontamination of radioactively-contaminated materials such as pipes and metal structures. The research and development of this technology was granted by the U.S. Department of Energy (DOE). Abrasion processes in vibratory vessels are widely used in the manufacture of metals, ceramics, and plastics. Samples to be treated, solid abrasive media and liquid media are set up into a vessel. Erosion results from the repeated impact of the abrasive particles on the surface of the body being treated. A liquid media, generally detergents or surfactants aid the abrasive action. The amount of material removed increases with the time of treatment. The design and construction of the machine were provided by Vibro, Argentina private company. Tests with radioactively-contaminated aluminum tubes and a stainless steel bar, were performed at laboratory level. Tests showed that it is possible to clean both the external and the internal surface of contaminated tubes. Results show a decontamination factor around 10 after the first 30 minutes of the cleaning time. (authors)

  16. Review of Artificial Abrasion Test Methods for PV Module Technology

    Energy Technology Data Exchange (ETDEWEB)

    Miller, David C. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Muller, Matt T. [National Renewable Energy Lab. (NREL), Golden, CO (United States); Simpson, Lin J. [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-08-01

    This review is intended to identify the method or methods--and the basic details of those methods--that might be used to develop an artificial abrasion test. Methods used in the PV literature were compared with their closest implementation in existing standards. Also, meetings of the International PV Quality Assurance Task Force Task Group 12-3 (TG12-3, which is concerned with coated glass) were used to identify established test methods. Feedback from the group, which included many of the authors from the PV literature, included insights not explored within the literature itself. The combined experience and examples from the literature are intended to provide an assessment of the present industry practices and an informed path forward. Recommendations toward artificial abrasion test methods are then identified based on the experiences in the literature and feedback from the PV community. The review here is strictly focused on abrasion. Assessment methods, including optical performance (e.g., transmittance or reflectance), surface energy, and verification of chemical composition were not examined. Methods of artificially soiling PV modules or other specimens were not examined. The weathering of artificial or naturally soiled specimens (which may ultimately include combined temperature and humidity, thermal cycling and ultraviolet light) were also not examined. A sense of the purpose or application of an abrasion test method within the PV industry should, however, be evident from the literature.

  17. Molecular Mechanism and Potential Targets for Blocking HPV-Induced Lesion Development

    OpenAIRE

    Madrid-Marina, V.; K. Torres-Poveda; Peralta-Zaragoza, O.; V. H. Bermúdez-Morales; E. Guzmán-Olea

    2011-01-01

    Persistent infection with high-risk HPV is the etiologic agent associated with the development of cervical cancer (CC) development. However, environmental, social, epidemiological, genetic, and host factors may have a joint influence on the risk of disease progression. Cervical lesions caused by HPV infection can be removed naturally by the host immune response and only a small percentage may progress to cancer; thus, the immune response is essential for the control of precursor lesions and C...

  18. Disseminated cutaneous sporotrichosis associated with ocular lesion in an immunocompetent patient*

    Science.gov (United States)

    Medeiros, Karina Bittencourt; Landeiro, Luana Gomes; Diniz, Lucia Martins; Falqueto, Aloísio

    2016-01-01

    A 59-year-old female patient, previously healthy, immunocompetent, presented left bulbar conjunctiva lesions and nodular-ulcerated lesions on the arms and cervical region, besides left cervical and retroauricular lymphadenopathy. She had previous contact with domestic cats that excoriated her face. The diagnosis was conclusive of disseminated sporotrichosis through clinical and epidemiological history and cultures of skin and ocular secretions. It evolved with good response to oral antifungal therapy. PMID:27579758

  19. Liquid-based cytologic screening on cervical lesion:a study of 38 088 cases%宫颈液基细胞学检查对宫颈病变的筛查价值-附38088例分析

    Institute of Scientific and Technical Information of China (English)

    贾英; 吴瑾; 唐良萏; 廖光东; 张新华

    2011-01-01

    目的:探讨液基细胞学检查(Thinprep cytology test,TCT)对宫颈病变的筛查价值.方法:回顾性分析2003年7月至2007年7月在重庆医科大学附属第一医院妇科38 088例TCT结果,阳性者定期复查或行阴道镜检查,必要时在阴道镜引导下定位活检.对有完整资料的1 556例病理结果按年龄进行分组分析,并对TCT与活检组织病理结果的相关性进行统计学分析.结果:38 088例TCT检测中,异常者2 462例,有明确阴道镜活检病理结果者1 556例,病理证实CINⅠ、CINⅡ、CINⅢ、宫颈癌及HPV感染病例分别为279、314、365、75例和94例.TCT与病理结果符合率为60.35%,TCT筛查宫颈病变的灵敏度为84%,特异度为53%,阳性预测值为83%,阴性预测值为56%.30~39岁为宫颈上皮内瘤变和人乳头瘤病毒的高发年龄段、宫颈癌的高发年龄段为30~49岁.TCT阳性者发生CINⅠ、CINⅡ和CINⅢ的相对危险度分别为:非典型鳞状细胞为11.11、13.21、8.19,鳞状上皮内低度病变为22.60、20.72、13.66,鳞状上皮内高度病变为10.33、25.45、54.65.结论:TCT筛查宫颈病变的阳性率、阳性预测值与活检病理结果符合率均较高,对病理结果有一定的预测价值,TCT阳性者宫颈病变的危险较一般人群明显升高.%To investigate the accuracy of Thin-Prep-liquid-based cytology test for cervical lesion with histopathological diagnosis as the criteria. Methods:TCT results of 38088 cases admitted to the First Affiliated Hospital of Chongqing Medical U- niversity from July 2003 to July 2007 were retrospectively studied. The patients with positive result underwent regular check or col- poscopy,and,if necessary,cervical biopsy under guidance of colposcope. A total of 1 556 samples with complete data were divided into groups and analyzed. The relativity of TCT results to biopsy pathological diagnosis was statistically analyzed. Results: The results of TCT examination for 38 088 cases indicated that 2 462

  20. Brushing abrasion of luting cements under neutral and acidic conditions.

    Science.gov (United States)

    Buchalla, W; Attin, T; Hellwig, E

    2000-01-01

    Four resin based materials (Compolute Aplicap, ESPE; Variolink Ultra, Vivadent; C&B Metabond, Parkell and Panavia 21, Kuraray), two carboxylate cements (Poly-F Plus, Dentsply DeTrey and Durelon Maxicap, ESPE), two glass-ionomer cements (Fuji I, GC and Ketac-Cem Aplicap, ESPE), one resin-modified glass ionomer cement (Vitremer, 3M) one polyacid-modified resin composite (Dyract Cem, Dentsply DeTrey) and one zinc phosphate cement (Harvard, Richter & Hoffmann) were investigated according to their brushing resistance after storage in neutral and acidic buffer solutions. For this purpose 24 cylindrical acrylic molds were each filled with the materials. After hardening, the samples were stored for seven days in 100% relative humidity and at 37 degrees C. Subsequently, they were ground flat and polished. Then each specimen was covered with an adhesive tape leaving a 4 mm wide window on the cement surface. Twelve samples of each material were stored for 24 hours in a buffer solution with a pH of 6.8. The remaining 12 samples were placed in a buffer with a pH of 3.0. All specimens were then subjected to a three media brushing abrasion (2,000 strokes) in an automatic brushing machine. Storage and brushing were performed three times. After 6,000 brushing strokes per specimen, the tape was removed. Brushing abrasion was measured with a computerized laser profilometer and statistically analyzed with ANOVA and Tukey's Standardized Range Test (p < or = 0.05). The highest brushing abrasion was found for the two carboxylate cements. The lowest brushing abrasion was found for one resin based material, Compolute Aplicap. With the exception of three resin-based materials, a lower pH led to a higher brushing abrasion.

  1. Wear characterization of abrasive waterjet nozzles and nozzle materials

    Science.gov (United States)

    Nanduri, Madhusarathi

    Parameters that influence nozzle wear in the abrasive water jet (AWJ) environment were identified and classified into nozzle geometric, AWJ system, and nozzle material categories. Regular and accelerated wear test procedures were developed to study nozzle wear under actual and simulated conditions, respectively. Long term tests, using garnet abrasive, were conducted to validate the accelerated test procedure. In addition to exit diameter growth, two new measures of wear, nozzle weight loss and nozzle bore profiles were shown to be invaluable in characterizing and explaining the phenomena of nozzle wear. By conducting nozzle wear tests, the effects of nozzle geometric, and AWJ system parameters on nozzle wear were systematically investigated. An empirical model was developed for nozzle weight loss rate. To understand the response of nozzle materials under varying AWJ system conditions, erosion tests were conducted on samples of typical nozzle materials. The effect of factors such as jet impingement angle, abrasive type, abrasive size, abrasive flow rate, water pressure, traverse speed, and target material was evaluated. Scanning electron microscopy was performed on eroded samples as well as worn nozzles to understand the wear mechanisms. The dominant wear mechanism observed was grain pullout. Erosion models were reviewed and along the lines of classical erosion theories a semi-empirical model, suitable for erosion of nozzle materials under AWJ impact, was developed. The erosion data correlated very well with the developed model. Finally, the cutting efficiency of AWJ nozzles was investigated in conjunction with nozzle wear. The cutting efficiency of a nozzle deteriorates as it wears. There is a direct correlation between nozzle wear and cutting efficiency. The operating conditions that produce the most efficient jets also cause the most wear in the nozzle.

  2. Cytological patterns of cervical pap smears with histopathological correlation

    Directory of Open Access Journals (Sweden)

    Bhagya Lakshmi Atla

    2015-08-01

    Conclusion: Pap smear is simple, inexpensive and can be performed in the outpatient department. Hence, it should be recommended routinely as a method of improving reproductive health, early detection of premalignant and malignant cervical lesions. [Int J Res Med Sci 2015; 3(8.000: 1911-1916

  3. European guidelines for quality assurance in cervical histopathology

    NARCIS (Netherlands)

    Bulten, J.; Horvat, R.; Jordan, J.; Herbert, A.; Wiener, H.; Arbyn, M.

    2011-01-01

    The current paper presents Chapter 5 of the second edition of the European Guidelines for Quality Assurance in Cervical Cancer Screening, which deals with the histopathological diagnosis of lesions of the uterine cervix. It completes a series of publications in journals containing the contents of ot

  4. Avaliação da sensibilidade e especificidade dos exames citopatológico e colposcópico em relação ao exame histológico na identificação de lesões intra-epiteliais cervicais Sensibility and specificity of cytology and colposcopy exams with the histological evaluation of cervical intraepithelial lesions

    Directory of Open Access Journals (Sweden)

    FELIPE FRANCISCO BONDAN TUON

    2002-06-01

    Maternidade Santa Brígida de Curitiba, PR, were selected. Those patients were sent to colposcopy under the following criteria: 1 previous abnormal cytopathological exams, 2 Indicative clinical data or, 3 Suspected lesions on gynecological exam. The statistical significance analysis of the results was done using the chi-square test. Sensibility, specificity, positive predictive value (PPV and negative predictive value (NPV were also determined.RESULTS: The patients' mean age was 30.2 (±10.9. Cytopathological capability of identifying lesions was 50% when compared to histology. Its specificity was 77%, the sensibility 41%, the PPV 74%, and the NPV 45%. Colposcopy capability of identifying lesions was 50%. Its sensibility, specificity, PPV and NPV were 96%, 19%, 65% and 75% respectively. The two methods associated were capable of identifying 63% of the lesions.CONCLUSIONS: Cytopathology was a high specificity exam, while colposcopy from those selected patients had a high sensibility. Colposcopy association with cytopathological screening, on those selected patients, significantly raises the diagnostic accuracy of cervical cancer precursor lesions.

  5. Screening for cervical cancer: when theory meets reality

    Directory of Open Access Journals (Sweden)

    Nygård Mari

    2011-06-01

    Full Text Available Abstract Cervical cancer screening reduces morbidity and mortality due to cervical cancer. However, there are many factors that determine the success of any cervical cancer prevention effort: the prevalence of human papillomavirus infection in general population, the existence of an organized screening program and the corresponding coverage, the existence and quality of the field and laboratory facilities for screening and diagnostic follow-up, and the facilities available for treating diagnosed lesions. Monitoring the patient path or "chain of action" for each patient with an abnormal screening result is of crucial importance. Cost-effectiveness models are widely used by decision-makers to determine which cervical cancer screening program would maximize health benefits within a given, usually limited, set of resources. Regardless of their level of sophistication, however, these models cannot replace empirical evaluations of the effectiveness of screening programs. Cervical cancer prevention activities need to be monitored and evaluated in each country where they are introduced to see that they meet performance standards. Policy-makers responsible for allocating resources for cervical cancer prevention have a duty to allocate resources not only for cervical cancer screening, but also for screening program surveillance.

  6. Radiological case: cervical teratoma

    OpenAIRE

    Macedo, F.

    2011-01-01

    We present a case of a third trimester fetus with a cervical mass. Fetal MRI was performed to better evaluate the extension of the mass and the risk of obstruction of the airway in the neonatal period. MRI is very useful in the evaluation of fetal cervical masses.

  7. CDC's Cervical Cancer Study

    Science.gov (United States)

    ... in Cancer Moonshot Stay Informed CDC’s Cervical Cancer Study Language: English Español (Spanish) Recommend on Facebook Tweet ... year. As part of CDC’s Cervical Cancer (Cx3) Study, we surveyed a sample of both health care ...

  8. Potential pitfall in the microfracturing technique during the arthroscopic treatment of an osteochondral lesion

    NARCIS (Netherlands)

    C.J.A. van Bergen; P.A.J. de Leeuw; C.N. van Dijk

    2009-01-01

    Debridement and bone marrow stimulation of the subchondral bone is currently considered to be the primary surgical treatment of most osteochondral lesions of the talus. Different methods of bone marrow stimulation are used, including drilling, abrasion, and microfracturing. The latter has gained rec

  9. Human papillomavirus types and recurrent cervical warts

    Energy Technology Data Exchange (ETDEWEB)

    Nuovo, G.J. (Columbia Presbyterian Medical Center, New York, NY (USA)); Pedemonte, B.M. (Harlem Hospital Medical Center, New York, NY (USA))

    1990-03-02

    The authors analyzed cervical intraepithelial neoplasias (CINs) detected after cryotherapy to determine if recurrence is associated with the same human papillomavirus (HPV) type found in the original lesion. Eight women had detectable HPV DNA in CINs that occurred after ablation of another CIN, and for each patient the HPV type in the pretreatment lesion was different from that in the CIN that appeared after cryotherapy. This compares with 12 women who had HPV detected in two or more CINs present at the same time, 11 of whom had the same HPv type noted. they concluded that although multiple, simultaneous CINs in a woman often contain the same HPV type, recurrent CINs that occur after cryotherapy contain an HPV type different from that present in the pretreatment lesion.

  10. Human papillomavirus types and recurrent cervical warts

    International Nuclear Information System (INIS)

    The authors analyzed cervical intraepithelial neoplasias (CINs) detected after cryotherapy to determine if recurrence is associated with the same human papillomavirus (HPV) type found in the original lesion. Eight women had detectable HPV DNA in CINs that occurred after ablation of another CIN, and for each patient the HPV type in the pretreatment lesion was different from that in the CIN that appeared after cryotherapy. This compares with 12 women who had HPV detected in two or more CINs present at the same time, 11 of whom had the same HPv type noted. they concluded that although multiple, simultaneous CINs in a woman often contain the same HPV type, recurrent CINs that occur after cryotherapy contain an HPV type different from that present in the pretreatment lesion

  11. HPVL1壳蛋白联合HPV分型检测在诊断子宫颈病变中的作用%HPV L1 protein unites HPV typing examination to diagnose cervical lesions

    Institute of Scientific and Technical Information of China (English)

    张露丹

    2011-01-01

    Objective To understind the HPV LI protein unites HPV typinge examination to diagnose the cervix pathological change. Methods The review from July, 2010 to January, 2011 carries on HPV in the second affiliated hospital of Zhengzhou University gynecoiogy department outpatient service the LI protein to unite HPV examination 403 patients were summarised the analyzed. Results When HPV LI protein and HPV typing examination ( - ) does not the found CIN 5 cases account for 0. 052% ; HPV LI protein and HPV typing examination( + ) in group CIN I 3 cases, CIN II 2 cases altogether account for 0. 086% ; In HPV LI protein( - ) , but HPVminute examination( + ) in group CIN I 59 cases, CIN II 37 cases, CIN Ⅲ 26 cases and the SCC 1 cases, the distinction accounts for 42.75% respectively, 26.81%, 18.84% and 0.007%. The fourth group has statistics significance with the first three group. Conclusions HPV LI protein and HPV typing examination in cervioal lesions has an important guiding funition, HPVLI protein deotction can be used as biological markers to forecast cevvical lesions progress.%目的 了解HPV L1壳蛋白联合HPV分型检测在诊断子宫颈病变中的作用.方法 回顾性的对在郑州大学第二附属医院妇科门诊2010年7月-2011年1月进行HPV L1壳蛋白联合HPV分型检测的403例患者进行总结和分析.结果 在HPV L1壳蛋白及HPV分型检测均(-)时没有CIN的出现;在HPV L1壳蛋白(+)而HPV分型检测(-)组中CIN Ⅰ有5例占0.052%;HPV L1壳蛋白及HPV分型检测均(+)组中CIN Ⅰ 3例、CINⅡ2例共占0.086;在HPV L1壳蛋白(-)而HPV分型检测(+)组CIN Ⅰ 59例Ⅱ37例、CINⅢ26例及SCC 1例,分别各占42.75%、26.81%、18.84%及0.007%.HpV L1壳蛋白(-)而HPV分型检测(+)组与前3组比较差异有统计学意义(P<0.05).结论 宫颈癌筛查十分必要,HPV L1壳蛋白联合HPV分型检测在诊断宫颈癌前病变中具有重要的指导作用,HPV L1壳蛋白检测可以作为预测官颈癌

  12. Preoperative Arterial Interventional Chemotherapy on Cervical Cancer

    Institute of Scientific and Technical Information of China (English)

    WANG Hui; LING HU-Hua; TANG Liang-dan; ZHANG Xing-hua

    2008-01-01

    Objective:To discuss the therapeutic effect of preoperative interventional chemotherapy on cervical cancer.Methods:Preoperative interventional chemotherapy by femoral intubation was performed in 25 patients with bulky cervical cancer.The patients received bleomycin 45 mg and cisplatin or oxaliplatin 80 mg/m2.Results:25 cases(including 8 cases with stage Ⅰ and 17 cases with stage Ⅱ)received one or two courses of preoperative interventional chemotherapy.The size of the focal lesions was decreased greatly and radical hysterectomy and lymphadenectomy were performed successfully in all the patients.All of the specimens were sent for pathological examination.Lymphocyte infiltration was found more obvious in the cancer tissues as compared with their counterpart before treatment.As a result,relevant vaginal bleeding was stopped completely shortly after the treatment.Conclusion:Arterial interventional chemotherapy was proved to reduce the local size of cervical cancer and thus control the hemorrhage efficiently.The patients with cervical cancer can receive radical hysterectomy therapy after the interventional chemotherapy.

  13. Study on abrasive mixing chamber of pre-mixed water jet

    OpenAIRE

    Junqing, Meng; Baisheng, Nie; Yechao, Ma; Bi, Zhao

    2015-01-01

    In order to reveal the flow law of isothermal, incompressible, steady, liquid-solid turbulent flow in mixing chamber of premixed abrasive water jet, the FLUENT software is used. The flow law of liquid-solid two phase flow is obtained. Abrasive mixing chamber models with four different sizes are used in this simulation, key design parameters of mine abrasive mixing chamber are determined. The flow laws of liquid-solid two phase flow in abrasive mixing chamber model with 7 different abrasive vo...

  14. MECHANISM AND PREDICTION OF MATERIAL ABRASION IN HIGH-VELOCITY SEDIMENT-LADEN FLOW

    Institute of Scientific and Technical Information of China (English)

    HUANG Xi-bin; YUAN Yin-zhong

    2006-01-01

    The wall surface of material is prone to silt abrasion by high-velocity sediment-laden flow. The silt abrasion is different form cavitation erosion. In this article, the characteristics of silt abrasion were discussed. The mechanism of silt abrasion was analyzed and the formation and development of ripple shape on wall surface of material were explained thereafter. Based on turbulence theory and test data, some formulas were derived for predicting the abrasion rate of concrete wall surface in high-velocity sediment-laden flow. The calculated results show good agreement with the experimental data.

  15. INDIVIDUALIZED TREATMENT OF PREINVASIVE LESIONS OF THE CERVIX

    Directory of Open Access Journals (Sweden)

    Neacsu Dorin

    2010-09-01

    Full Text Available The management of cervical preinvasive lesions in primary medical practice is characterized by a wide variety of attitudes, objectives and therapeutic decisions. The purpose of the study. To supply new landmarks referring to the preinvasive lesions of cervical cancer from the perspective of the clinician. Materials and methods. The study was made on a number of 16732 patients who were monitored comparatively in the Family Planning offices in the counties of Ia�i and Buz�u, between 2007 and 2011. Results and discussions. The treatment was applied according to the type of the lesion. Conclusions. The individualized treatment of the preinvasive lesions of the cervix targeted the destruction of the pathogenic agent and the infected tissues and caused a fibroblast proliferation and re-epithelisation

  16. Role of IL-10 and TGF-β1 in local immunosuppression in HPV-associated cervical neoplasia

    OpenAIRE

    Torres-Poveda, Kirvis; Bahena-Román, Margarita; Madrid-González, Claudia; Ana I Burguete-García; Bermúdez-Morales, Víctor Hugo; Peralta-Zaragoza, Oscar; Madrid-Marina, Vicente

    2014-01-01

    Cervical cancer is a worldwide disease that constitutes a significant public health problem, especially in developing countries, not only due to its high incidence but also because the most affected population comprises women who belong to marginalized socio-economic classes. Clinical and molecular research has identified immunological impairment in squamous intraepithelial cervical lesions and cervical cancer patients. Human Papillomavirus (HPV) has several mechanisms for avoiding the immune...

  17. Application of combined detection of HR-HPV and TCT in screening for cervical cancer and precancerous lesions%高危型人乳头瘤病毒检测联合薄层液基细胞学检查在宫颈疾病筛查中的应用

    Institute of Scientific and Technical Information of China (English)

    叶波; 宋晓玉

    2015-01-01

    目的:进行高危型人乳头瘤病毒(HR-HPV)检测,分析液基薄层细胞学(TCT)检测与组织病理学诊断结果的符合情况,探讨二者联合检测对宫颈癌及癌前病变的筛查价值和意义。方法对1374例妇瘤科就诊患者,进行 HR-HPV、TCT 联合检测,对结果进行对比分析。结果684例意义不明的不典型鳞状细胞(ASCUS)患者病理学诊断结果中,炎症占51.3%,不典型增生轻度(CINⅠ)占47.4%;375例低度鳞状上皮内病变(LSIL)患者病理学诊断结果中,CINⅠ占48.5%,CINⅡ~Ⅲ占21.3%;294例高度鳞状上皮内病变(HSIL)患者病理学诊断结果中,CINⅠ占24.5%,CINⅡ~Ⅲ占62.9%;20例 SCC 患者病理学诊断结果中,CINⅡ~Ⅲ占20%,宫颈癌占80%。统计学分析显示,TCT 诊断与组织病理学诊断构成比差异有统计学意义(P <0.05)。HR-HPV 阳性率为74.3%,阴性率为25.7%。HR-HPV 阳性率随病理级别的升高而显著升高。HR-HPV 阳性组与阴性组病理诊断级别构成比差异有统计学意义(P <0.05)。TCT 与高危型 HPV 联合检测时,其灵敏度为76.5%,特异度为80.3%,联合检测的灵敏度和 TCT 差异有统计学意义(P <0.05),联合检测的特异度和 HR-HPV 差异有统计学意义(P <0.05)。结论 HR-HPV 检测与 TCT 联合能够提高宫颈病变的检出率,是一种较为理想的宫颈癌筛查方法。%Objective To study the status of coincidence of high-risk human papillomavirus(HR-HPV)test and thinprep cytolo-gy test with biopsy histopathologic diagnosis.And to investigate the value of combined detection in the diagnosis of the cervical cancer and precancerous lesions.Methods High-risk HPV test and thinprep cytology test were taken simultaneously in 1374 pa-tients visiting department of gynecological oncology.The diagnostic value of combination assay of these indices was evaluated.Re-sults In 684 patients with ASCUS pathology diagnosis,inflammation accounted for 5 1.3% and CINⅠ accounted for 47.4%.A-mong the

  18. 人乳头瘤病毒E6/E7mRNA检测在宫颈病变筛查中的应用价值%The application value of human papillomavirus E6/E7 mRNA detection in cervical lesions screening

    Institute of Scientific and Technical Information of China (English)

    张生枝; 邵华江; 马建婷

    2014-01-01

    目的:探讨人乳头瘤病毒(HPV)E6/E7 mRNA 检测在宫颈病变筛查中的应用价值。方法2012年6月至2013年4月在余姚市人民医院因宫颈炎症、阴道异常出血或排液的720例门诊患者行宫颈 TCT 和 HPV DNA 检测,对 TCT 结果≥ASC-US 或(和)高危 HPV DNA 阳性者行宫颈 HPV E6/E7mRNA 检测和组织病理学检查。以病理学结果为标准,与 HPV DNA 检测相比较,评价 HPV E6/E7 mRNA 检测对 CIN2+诊断价值,并用受试者工作特征(ROC)曲线评价其准确性。结果在 CIN 和宫颈浸润癌中,HPV E6/E7 mRNA 总体阳性率为78.7%,低于 HPV DNA 总体阳性率(92.6%),差异有统计学意义(P <0.001)。HPV E6/E7 mRNA 检测诊断 CIN2+的灵敏度为83.0%,低于 HPV DNA(94.5%),差异有统计学意义(P <0.01);特异度为51.1%,高于 HPV DNA(22.8%),差异有统计学意义(P <0.001)。 HPV E6/E7 mRNA、HPV DNA 对诊断 CIN2+的 ROC 曲线下面积分别为0.670、0.587, HPV E6/E7 mRNA 检测对 CIN2+的诊断的准确性高于 HPV DNA,差异有统计学意义(P <0.01)。结论 HPV E6/E7 mRNA 检测诊断 CIN2+特异度和准确性高于 HPV DNA,对宫颈病变的筛查有一定应用价值。%Objective To investigate the application value of detecting human papillomavirus(HPV)E6/E7 mRNA as a diagnose test for cervical lesions screening.Methods From June 2012 to April 2013,a total of 720 gynecological outpatients in Yangming Hospital affiliated to Medical School of Ningbo University,who had cervical inflammation,ab-normal vaginal bleeding or discharge ,received TCT and HPV DNA tests,and for those whose TCT≥ASC-US or/and high-risk HPV DNA-positive had HPV E6/E7 mRNA detection and histopathological examination.With the pathology results as the standard,the value of HPV E6/E7 mRNA detection for the diagnosis of CIN2+ was evaluated by com-parison with the HPV DNA test,and its

  19. The utility of diffusion-weighted MR imaging in cervical cancer

    Energy Technology Data Exchange (ETDEWEB)

    Chen Jianyu, E-mail: Chenjianyu5562@163.co [Department of Radiology, Second Affiliated Hospital, Sun Yat-sen University, 107 Yanjiangxi Road, Guangzhou 510120 (China); Zhang Yun, E-mail: Zhangyun.7@163.co [State Key Laboratory of Oncology in Southern China, Guangzhou, 510060 (China) and Imaging Diagnosis and Interventional Center, Cancer Center, Sun Yat-sen University, Guangzhou, 510060 (China); Liang Biling, E-mail: liangbil@126.co [Department of Radiology, Second Affiliated Hospital, Sun Yat-sen University, 107 Yanjiangxi Road, Guangzhou 510120 (China); Yang Zehong, E-mail: Yangzehong2000@163.co [Department of Radiology, Second Affiliated Hospital, Sun Yat-sen University, 107 Yanjiangxi Road, Guangzhou 510120 (China)

    2010-06-15

    Purpose: To investigate the value of diffusion-weighted MR imaging (DWI) in detection of cervical cancer, and to determine the diagnostic accuracy of apparent diffusion coefficient (ADC) values for evaluating cervical cancer before and after chemoradiotherapy. Materials and methods: Thirty-three patients with cervical squamous carcinoma and 20 patients with other pelvic abnormalities underwent diffusion-weighted imaging (DWI) in addition to routine MR imaging. The ADC values of normal cervical tissue, cervical area before and after chemoradiotherapy were measured and compared. Receiver operating characteristic (ROC) analysis was employed to investigate whether ADC values could help in discrimination among normal cervical tissue, cervical cancer before and after therapy, and to obtain the optimal ADC threshold value. Results: Cervical cancer lesion demonstrated obviously hyperintensity on DWI images. The mean ADC value of cervical carcinoma (1.110 {+-} 0.175 x 10{sup -3} mm{sup 2}/s) was significantly lower than that of normal cervical tissue (1.593 {+-} 0.151 x 10{sup -3} mm{sup 2}/s) (P < 0.001). The mean ADC value of the cervical area in 22 patients treated by chemoradiotherapy (1.436 {+-} 0.129 x 10{sup -3} mm{sup 2}/s) was significantly higher than that before therapy (1.013 {+-} 0.094 x 10{sup -3} mm{sup 2}/s) (P < 0.001). The difference of ADC values between normal cervical tissue and cervical area after therapy was statistically significant (P < 0.01). The optimal ADC threshold values for distinguishing between normal cervical tissue and cervical carcinoma was 1.359 x 10{sup -3} mm{sup 2}/s, between cervical area before and after therapy was 1.255 x 10{sup -3} mm{sup 2}/s, between normal cervical tissue and cervical area after therapy was 1.525 x 10{sup -3} mm{sup 2}/s. The sensitivity and specificity were 100% and 84.8%, 95.5% and 100%, 70% and 81.8%, respectively. Conclusion: DWI can be applied for the detection of cervical cancer because of its superior

  20. MRI and PET Imaging in Predicting Treatment Response in Patients With Stage IB-IVA Cervical Cancer

    Science.gov (United States)

    2016-06-24

    Cervical Adenocarcinoma; Cervical Adenosquamous Carcinoma; Cervical Squamous Cell Carcinoma; Cervical Undifferentiated Carcinoma; Recurrent Cervical Carcinoma; Stage IB2 Cervical Cancer; Stage IIA Cervical Cancer; Stage IIB Cervical Cancer; Stage IIIA Cervical Cancer; Stage IIIB Cervical Cancer; Stage IVA Cervical Cancer

  1. Clinical and pathological heterogeneity of cervical intraepithelial neoplasia grade 3.

    Directory of Open Access Journals (Sweden)

    Hannah P Yang

    Full Text Available OBJECTIVE: Cervical intraepithelial neoplasia grade 3 (CIN3, the immediate cervical cancer precursor, is a target of cervical cancer prevention. However, less than half of CIN3s will progress to cancer. Routine treatment of all CIN3s and the majority of CIN2s may lead to overtreatment of many lesions that would not progress. To improve our understanding of CIN3 natural history, we performed a detailed characterization of CIN3 heterogeneity in a large referral population in the US. METHODS: We examined 309 CIN3 cases in the SUCCEED, a large population-based study of women with abnormal cervical cancer screening results. Histology information for 12 individual loop electrosurgical excision procedure (LEEP segments was evaluated for each woman. We performed case-case comparisons of CIN3s to analyze determinants of heterogeneity and screening test performance. RESULTS: CIN3 cases varied substantially by size (1-10 LEEP segments and by presentation with concomitant CIN2 and CIN1. All grades of CINs were equally distributed over the cervical surface. In half of the women, CIN3 lesions were found as multiple distinct lesions on the cervix. Women with large and solitary CIN3 lesions were more likely to be older, have longer sexual activity span, and have fewer multiple high risk HPV infections. Screening frequency, but not HPV16 positivity, was an important predictor of CIN3 size. Large CIN3 lesions were also characterized by high-grade clinical test results. CONCLUSIONS: We demonstrate substantial heterogeneity in clinical and pathological presentation of CIN3 in a US population. Time since sexual debut and participation in screening were predictors of CIN3 size. We did not observe a preferential site of CIN3 on the cervical surface that could serve as a target for cervical biopsy. Cervical cancer screening procedures were more likely to detect larger CIN3s, suggesting that CIN3s detected by multiple independent diagnostic tests may represent cases

  2. Comparison of two commercial assays for detection of human papillomavirus (HPV) in cervical scrape specimens: validation of the Roche AMPLICOR HPV test as a means to screen for HPV genotypes associated with a higher risk of cervical disorders.

    NARCIS (Netherlands)

    Ham, M.A. van; Bakkers, J.M.J.E.; Harbers, G.; Quint, W.G.V.; Massuger, L.F.A.G.; Melchers, W.J.G.

    2005-01-01

    Certain high-risk (HR) human papillomavirus (HPV) types are a necessary cause for the development of cervical disorders. Women with persistent HR HPV infections have an increased risk of developing high-grade cervical lesions, compared with those who have no or low-risk HPV infections. Therefore, im

  3. 不能明确意义的不典型鳞状细胞伴DNA倍体异常在宫预早期病变筛查中的意义%Diagnostic implications of atypical squamous cells of unknown significance with abnormal DNA ploidy for early cervical lesions

    Institute of Scientific and Technical Information of China (English)

    梅金红; 徐姗; 韩永良; 涂轶; 熊一峰; 余燕青

    2013-01-01

    Objective To investigate the clinical significance of atypical squamous cells of unknown significance (ASCUS) with abnormal DNA ploidy in the early diagnosis of cervical lesions.Methods Eight thousand four hundred and forty-eight patients were included in this study and all had DNA quantitative analysis and cervical liquid-based cytology.Among 1041 cases with DNA aneuploidy and/or abnormal cervical liquid-based cytology and additional cervical biopsy,histological review was performed in 247 ASCUS cases with abnormal DNA ploidy.Results (1) Among 8448 cases,7877 were normal or benign,426 were ASCUS,45 were ASC-H,55 were LSIL and 22 were HSIL by TBS diagnosis.The presence of 1-2 abnormal DNA ploidy cells was detected in 15.3% (65/426) of ASCUS,11.1% (5/45) of ASC-H,9.1% (5/55) of LSIL,and 0 (0/22) of HSIL.The presence of ≥ 3 abnormal DNA ploidy cells was detected in 39.0% (166/426) of ASCUS,75.6% (34/45) of ASC-H,76.4% (42/55) of LSIL,and 95.5% (21/22) of HSIL.(2) A total of 67 cases of CIN 2,CIN 3 or cancers were found in 247 patients with ASCUS by colposcopy biopsies,of which 13.9% (5/36) had 1-2 abnormal DNA ploidy cells,45.5% (56/123) had ≥3 abnormal DNA ploidy cells and 6.8% (6/88) had normal DNA polidy.ASCUS with 1-2 abnormal DNA ploidy cells and with ≥ 3 abnormal DNA ploidy cells were compared.The difference was statistically significant (x2 =11.79,P <0.01).But the difference between ASCUS with 1-2 abnormal DNA ploidy cells and normal DNA ploidy had no statistical significance (P > 0.05).Conclusions ASCUS with ≥3 abnormal DNA ploidy cells has higher risk for developing CIN 2,CIN 3 or invasive carcinoma.The application of DNA quantitative analysis and cervical liquid-based cytology test can help in guiding clinical follow-up and treatment options in patients with ASCUS.%目的 通过DNA定量分析与薄层液基细胞学检查,探讨不能明确意义的不典型鳞状细胞(ASCUS)伴DNA倍体异常在宫颈早期

  4. Cervical involvement in SAPHO syndrome: imaging findings with a 10-year follow-up

    Energy Technology Data Exchange (ETDEWEB)

    Tohme-Noun, C.; Krainik, A.; Menu, Y. [Department of Radiology, Hopital Beaujon, AP HP, Universite Paris 7, Faculte de medecine Bichat-Beaujon, Paris (France); Feydy, A. [Department of Radiology, Hopital Beaujon, AP HP, Universite Paris 7, Faculte de medecine Bichat-Beaujon, Paris (France); Department of Radiology, Hopital Beaujon, 100 avenue du General Leclerc, 92118, Clichy (France); Belmatoug, N.; Fantin, B. [Department of Internal Medicine, Hopital Beaujon, AP HP, Universite Paris 7, Faculte de medecine Bichat-Beaujon, Paris (France)

    2003-02-01

    Osteoarticular manifestations of SAPHO syndrome include vertebral lesions, typically in the thoracic segment. Chronic inflammatory changes are well depicted by MRI. We report the imaging findings with a 10-year follow-up in a case of SAPHO syndrome with marked cervical lesions. (orig.)

  5. 血清可溶性白细胞G抗原在宫颈癌及癌前病变的临床诊断意义%Clinical diagnostic value of plasma sHLA-G antigen in cervical cancer and precancerous lesion

    Institute of Scientific and Technical Information of China (English)

    田玫玲; 卢庆乐

    2015-01-01

    断指标。但其与发生宫颈癌及癌前病变的特异性分析仍有待进一步的相关研究。%Objective To explore the clinical diagnostic valve of serum soluble human leukocyte antigen‐G (sHLA‐G) in cervical cancer and precancerous lesion cervical intraepithelial neoplasia(CIN) .Methods The serum sHLA‐G level was detected by using ELISA and serum TA‐4 and SCC‐Ag levels were detected by using the light‐emitting electrochemical immunoassay method detec‐ting in 230 cases of cervical carcinoma ,120 cases of CIN and 30 healthy volunteers .The differences among various groups and their relationship with the clinicopathological features of cervical cancer were analyzed .Results (1) The comparison of serum sHLA‐G , TA‐4 and SCC‐Ag levels :there were statistically significant differences in serum sHLA‐G level among various groups (P=0 .000);the serum sHLA‐G level in the cervical cancer group was significantly higher than that in the healthy control group ,CIN Ⅰgroup , CIN Ⅱ group and CIN Ⅲ group (P=0 .000 ,P=0 .000 ,P=0 .002 ,P=0 .006);which in the CIN Ⅲ group was significantly higher than that in the CIN Ⅰ group and the healthy control group (P=0 .001 ,P=0 .021) .There were statistically significant differences in serum TA‐4 level among various groups (P=0 .006);the serum TA‐4 level in the cervical cancer group was significantly higher than that in the healthy control group ,CIN Ⅰ group and CIN Ⅱ group (P=0 .003 ,P=0 .008 ,P=0 .018);which in the CIN Ⅲgroup was significantly higher than that in the healthy control group and the CIN Ⅰ group (P=0 .023 ,P=0 .031) .The differences of serum SCC‐Ag level among various groups had statistically significant differences (P=0 .000);which in the cervical cancer group was significantly higher than that in the healthy control group ,CIN Ⅰ group and CIN Ⅱ group (P=0 .000 ,P=0 .001 ,P=0 .007) , and which in the CIN Ⅲ group was significantly higher than that in the healthy

  6. Priority Setting for Improvement of Cervical Cancer Prevention in Iran

    Directory of Open Access Journals (Sweden)

    Azam Majidi

    2016-04-01

    Full Text Available Background Cervical cancer is the fourth most common cancer among women worldwide. Organized cervical screening and vaccination against human papilloma virus (HPV have been successful interventions for prevention of invasive cervical cancer (ICC. Because of cultural and religious considerations, ICC has low incidence in Iran and many other Muslim countries. There is no organized cervical screening in these countries. Therefore, ICC is usually diagnosed in advanced stages with poor prognosis in these countries. We performed a priority setting exercise and suggested priorities for prevention of ICC in this setting. Methods We invited experts and researchers to a workshop and asked them to list important suggestions for ICC prevention in Iran. After merging similar items and removing the duplicates, we asked the experts to rank the list of suggested items. We used a strategy grid and Go-zone analysis to determine final list of priorities for ICC prevention in Iran. Results From 26 final items suggested as priorities for prevention of ICC, the most important priorities were developing national guidelines for cervical screening and quality control protocol for patient follow-up and management of precancerous lesions. In addition, we emphasized considering insurance coverage for cervical screening, public awareness, and research priorities, and establishment of a cervical screening registry. Conclusion A comprehensive approach and implementation of organized cervical screening program is necessary for prevention of ICC in Iran and other low incidence Muslim countries. Because of high cost for vaccination and low incidence of cervical cancer, we do not recommend HPV vaccination for the time being in Iran.

  7. Inspeção visual do colo uterino após aplicação de ácido acético no rastreamento das neoplasias intra-epiteliais e lesões induzidas por HPV Cervical visual inspection after application of acetic acid in screening intraepithelial neoplasia and HPV-induced lesions

    Directory of Open Access Journals (Sweden)

    Maria Rachel Aguiar Cordeiro

    2005-02-01

    Full Text Available OBJETIVO: estimar a validade da inspeção visual após aplicação de ácido acético (IVA no rastreamento das neoplasias intra-epiteliais cervicais (NIC e lesões induzidas por HPV, comparando seu desempenho com o da colpocitologia e da colposcopia. MÉTODOS: estudo de validação de teste diagnóstico realizado em 893 mulheres de 18 a 65 anos, rastreadas simultaneamente com colpocitologia, IVA e colposcopia, em unidade de saúde pública de Recife, PE. A IVA foi realizada por embrocação do colo com ácido acético a 5% e observação a olho nu, com auxílio de foco clínico comum. Considerou-se como positividade o achado de qualquer lesão aceto-branca no colo. O padrão-ouro foi o histopatológico de biópsia cervical, realizado sempre que qualquer um dos três testes resultasse anormal. Foram estimados e comparados os indicadores de validade de cada teste, com os respectivos intervalos de confiança a 95%. A concordância entre os resultados dos testes foi avaliada pelo coeficiente kappa (k. RESULTADOS: das 303 mulheres biopsiadas, o estudo histopatológico foi anormal em 24. Deste total, a IVA foi positiva em 22, conferindo-lhe sensibilidade estimada de 91,7%, especificidade de 68,9%, valor preditivo positivo de 7,5% e valor preditivo negativo de 99,7%. Comparando-se os intervalos de confiança a 95%, a IVA mostrou maior sensibilidade que a colpocitologia, mas com menores especificidade e valor preditivo positivo. Houve fraca concordância entre os resultados da IVA e da colpocitologia (k=0,02 e excelente concordância com os da colposcopia (k=0,93. CONCLUSÃO: a IVA foi muito mais sensível que a colpocitologia no rastreamento das NIC e lesões HPV-induzidas e teve o mesmo desempenho da colposcopia. Sua baixa especificidade foi responsável por um elevado número de resultados falso-positivos.PURPOSE: to estimate the validity of visual inspection of cervical intraepithelial neoplasia (CIN and HPV-induced lesion screening, after acetic

  8. HPV-Based Screening, Triage, Treatment, and Followup Strategies in the Management of Cervical Intraepithelial Neoplasia

    Directory of Open Access Journals (Sweden)

    Oscar Peralta-Zaragoza

    2013-01-01

    Full Text Available Cervical cancer is the second most common cause of death from cancer in women worldwide, and the development of new diagnostic, prognostic, and treatment strategies merits special attention. Many efforts have been made to design new drugs and develop immunotherapy and gene therapy strategies to treat cervical cancer. HPV genotyping has potentially valuable applications in triage of low-grade abnormal cervical cytology, assessment of prognosis and followup of cervical intraepithelial neoplasia, and in treatment strategies for invasive cervical cancer. It is known that during the development of cervical cancer associated with HPV infection, a cascade of abnormal events is induced, including disruption of cellular cycle control, alteration of gene expression, and deregulation of microRNA expression. Thus, the identification and subsequent functional evaluation of host proteins associated with HPV E6 and E7 oncoproteins may provide useful information in understanding cervical carcinogenesis, identifying cervical cancer molecular markers, and developing specific targeting strategies against tumor cells. Therefore, in this paper, we discuss the main diagnostic methods, management strategies, and followup of HPV-associated cervical lesions and review clinical trials applying gene therapy strategies against the development of cervical cancer.

  9. HPV-Based Screening, Triage, Treatment, and Followup Strategies in the Management of Cervical Intraepithelial Neoplasia.

    Science.gov (United States)

    Peralta-Zaragoza, Oscar; Deas, Jessica; Gómez-Cerón, Claudia; García-Suastegui, Wendy Argelia; Fierros-Zárate, Geny Del Socorro; Jacobo-Herrera, Nadia Judith

    2013-01-01

    Cervical cancer is the second most common cause of death from cancer in women worldwide, and the development of new diagnostic, prognostic, and treatment strategies merits special attention. Many efforts have been made to design new drugs and develop immunotherapy and gene therapy strategies to treat cervical cancer. HPV genotyping has potentially valuable applications in triage of low-grade abnormal cervical cytology, assessment of prognosis and followup of cervical intraepithelial neoplasia, and in treatment strategies for invasive cervical cancer. It is known that during the development of cervical cancer associated with HPV infection, a cascade of abnormal events is induced, including disruption of cellular cycle control, alteration of gene expression, and deregulation of microRNA expression. Thus, the identification and subsequent functional evaluation of host proteins associated with HPV E6 and E7 oncoproteins may provide useful information in understanding cervical carcinogenesis, identifying cervical cancer molecular markers, and developing specific targeting strategies against tumor cells. Therefore, in this paper, we discuss the main diagnostic methods, management strategies, and followup of HPV-associated cervical lesions and review clinical trials applying gene therapy strategies against the development of cervical cancer. PMID:23690785

  10. Optimization of Abrasive Water Jet Cutting of Ductile Materials

    Institute of Scientific and Technical Information of China (English)

    Asif IQBAL; Naeem U DAR; Ghulam HUSSAIN

    2011-01-01

    Full factorial design of experiments was developed in order to investigate the effects of jet pressure, abrasive mixing rate, cutting feed, and plate thickness upon three response variables, surface finish of cutting wear zone, percentage proportion of striation free area, and maximum width of cut. The set of sixteen experiments was performed on each of the following two ductile materials: AISI 4340 (high strength low alloy steel, hardened to 49HRc) and Aluminum 2219. Analysis of Variance (ANOVA) was performed on experimental data in order to determine the significance of effects of different parameters on the performance measures. It was found that cutting feed and thickness were highly influential parameters, while abrasive mixing rate is influential upon surface roughness only. Strong interaction was found between jet pressure and workpiece material.Multi-criteria numerical optimization was performed in order to simultaneously maximize/minimize different combinations of performance measures.

  11. Abrasion Testing of Critical Components of Hydrokinetic Devices

    Energy Technology Data Exchange (ETDEWEB)

    Worthington, Monty [ORPC Alaska; Ali, Muhammad [Ohio University; Ravens, Tom [University of Alaska Anchorage

    2013-12-06

    The objective of the Abrasion Testing of Critical Components of Hydrokinetic Devices (Project) was to test critical components of hydrokinetic devices in waters with high levels of suspended sediment – information that is widely applicable to the hydrokinetic industry. Tidal and river sites in Alaska typically have high suspended sediment concentrations. High suspended sediment also occurs in major rivers and estuaries throughout the world and throughout high latitude locations where glacial inputs introduce silt into water bodies. In assessing the vulnerability of technology components to sediment induced abrasion, one of the greatest concerns is the impact that the sediment may have on device components such as bearings and seals, failures of which could lead to both efficiency loss and catastrophic system failures.

  12. Nanometric Finishing on Biomedical Implants by Abrasive Flow Finishing

    Science.gov (United States)

    Subramanian, Kavithaa Thirumalai; Balashanmugam, Natchimuthu; Shashi Kumar, Panaghra Veeraiah

    2016-01-01

    Abrasive flow finishing (AFF) is a non-conventional finishing technique that offers better accuracy, efficiency, consistency, economy in finishing of complex/difficult to machine materials/components and provides the possibility of effective automation as aspired by the manufacturing sector. The present study describes the finishing of a hip joint made of ASTM grade Co-Cr alloy by Abrasive Flow Machining (AFM) process. The major input parameters of the AFF process w